{ "genome": { "genus": "Ureaplasma", "species": "parvum", "strain": "ATCC 700970", "taxon": "Ureaplasma parvum ATCC 700970", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 751719, "gc": 0.2549968804832657, "n_ratio": 0.0, "n50": 751719, "n90": 751719, "coding_ratio": 0.7075223587537365 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 1, "stop": 990, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00308.24", "PFAM:PF01695.23" ], "nt": "ATGGCTAATAATTATCAAACTTTATATGATTCAGCAATAAAAAGGATTCCATACGATCTTATTTCTGATCAAGCTTATGCAATTCTACAAAATGCTAAAACTCATAAAGTTTGCGATGGTGTTTTATATATAATTGTAGCCAATGCCTTTGAAAAAAGTATTATTAACGGTAATTTTATTAACATTATTTCTAAATATCTAAGCGAAGAATTCAAAAAGGAAAATATTGTTAATTTTGAATTTATTATAGACAATGAAAAATTATTAATTAATAGCAATTTTTTAATTAAAGAAACTAATATTAAAAATCGTTTTAATTTTAGTGATGAACTTTTACGTTACAATTTTAACAATTTAGTAATTAGTAATTTTAATCAAAAAGCGATTAAGGCGATTGAAAATTTATTTTCAAATAACTATGATAATAGTTCAATGTGTAACCCTTTATTTTTATTTGGTAAAGTTGGTGTTGGTAAAACGCATATCGTGGCTGCTGCTGGTAATCGTTTTGCTAATAGTAATCCTAATTTAAAAATTTATTATTATGAAGGGCAAGATTTTTTTCGAAAGTTTTGTTCTGCTTCGTTAAAAGGGACTAGTTATGTTGAAGAGTTTAAAAAAGAAATTGCTTCAGCAGATTTATTAATTTTTGAAGATATTCAAAATATCCAATCACGTGATTCAACGGCTGAATTGTTTTTTAATATCTTTAATGATATAAAATTAAATGGTGGAAAAATTATCTTAACATCTGACCGTACACCAAACGAACTTAATGGTTTTCATAATCGAATTATTTCGAGATTAGCGTCAGGTTTGCAGTGTAAAATTTCTCAACCCGACAAAAATGAAGCTATTAAAATTATTAATAATTGGTTTGAATTCAAAAAAAAATATCAAATTACTGACGAAGCTAAAGAATATATTGCTGAAGGTTTTCACACTGATATTAGACAGATGATTGGTAATCTAAAACAAATTTGTTTTTGA", "aa": "MANNYQTLYDSAIKRIPYDLISDQAYAILQNAKTHKVCDGVLYIIVANAFEKSIINGNFINIISKYLSEEFKKENIVNFEFIIDNEKLLINSNFLIKETNIKNRFNFSDELLRYNFNNLVISNFNQKAIKAIENLFSNNYDNSSMCNPLFLFGKVGVGKTHIVAAAGNRFANSNPNLKIYYYEGQDFFRKFCSASLKGTSYVEEFKKEIASADLLIFEDIQNIQSRDSTAELFFNIFNDIKLNGGKIILTSDRTPNELNGFHNRIISRLASGLQCKISQPDKNEAIKIINNWFEFKKKYQITDEAKEYIAEGFHTDIRQMIGNLKQICF", "aa_hexdigest": "37fdf62d77110ca8a53eab2cced72a7e", "start_type": "Edge", "rbs_motif": null, "truncated": "5-prime", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00308.24", "name": "Bac_DnaA", "length": 163, "aa_cov": 0.49544072948328266, "hmm_cov": 0.9693251533742331, "evalue": 1.1409663599938922e-39, "score": 131.76156616210938, "start": 113, "stop": 275 }, { "id": "PF01695.23", "name": "IstB_IS21", "length": 124, "aa_cov": 0.3768996960486322, "hmm_cov": 0.9435483870967742, "evalue": 1.7693277564596032e-09, "score": 33.04195785522461, "start": 131, "stop": 254 } ], "seq_stats": { "molecular_weight": 37917.7531, "isoelectric_point": 8.105690574645994 }, "id": "GPKLONFDEH_1301", "locus": "GCA000006625_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1515, "stop": 1802, "strand": "+", "frame": 3, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_006688758.1", "SO:0001217", "UniParc:UPI0000133F35", "UniRef:UniRef100_Q9PRE1", "UniRef:UniRef50_Q9PRE1", "UniRef:UniRef90_Q9PRE1" ], "nt": "ATGAGAGATATTCATCCAGTAAGCAAACCATGCGTTTATAATTGTGTAACATGTAAAAAAGAATTTATAATTGATTCTGCAGCAAAAAATACAGAAGTTGCAATTGAAGTTTGTTCAAATTGCCATACATTTTTTATTGGTAAACAAAACGCAACTACAACATTACGTGGTCGTGCTGAAAAACTAAATAATCGTTTTGAAGCTGGTTTAAATAACATTAATAAAAAACCAGAAAAGAAAAAAATACAAGGTAAATCTGAACCAAGAAAAAGTTTAAACGAATTATAA", "aa": "MRDIHPVSKPCVYNCVTCKKEFIIDSAAKNTEVAIEVCSNCHTFFIGKQNATTTLRGRAEKLNNRFEAGLNNINKKPEKKKIQGKSEPRKSLNEL", "aa_hexdigest": "17dbc090192fce23910a07d4469078fb", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000133F35", "ncbi_nrp_id": "WP_006688758.1", "uniref100_id": "UniRef100_Q9PRE1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133F35", "RefSeq:WP_006688758.1", "UniRef:UniRef100_Q9PRE1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRE1", "uniref90_id": "UniRef90_Q9PRE1" }, "psc": { "uniref90_id": "UniRef90_Q9PRE1", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_Q9PRE1", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRE1" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "GPKLONFDEH_42", "locus": "GCA000006625_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1935, "stop": 2375, "strand": "+", "frame": 3, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_Q9PRE0", "UniRef:UniRef90_Q9PRE0" ], "nt": "ATGGAATACAACAAAAAACTTTATGAAGCAATTGAACGAGTCGCTATTAAAAATGACGCTTTAAAAAAAGAATTAGAAACGGTTGTAACAGACTTTAAAAAAATTAAAGAGATTAATATACAGTTAAAAAAAACTACTAAAATTGCTGAAGCCTTTGCAAAATATAAACAAAAATTGGATACTGGTATAGCTGCTGAAAAAATATTAAATACAGAAAAAGATTTAGAATTAATCGAATTAGCACAGATGGATTTAGATGAAGCTAAAATTAATATTCCAATAATTGAAAATGATTTAAAAATTATGCTGTTGCCAACTGATCCTAATGATGATAAAAATGTTATTGTTGAAATGCGCCCTGCTGCTGGAGGGGATGAATCATCAATTTTTGTCGGCAATTTGTTTGATACATATCGTGCTTATGCTGAAAATAATAATTGA", "aa": "MEYNKKLYEAIERVAIKNDALKKELETVVTDFKKIKEINIQLKKTTKIAEAFAKYKQKLDTGIAAEKILNTEKDLELIELAQMDLDEAKINIPIIENDLKIMLLPTDPNDDKNVIVEMRPAAGGDESSIFVGNLFDTYRAYAENNN", "aa_hexdigest": "0fb5020b617e12e510ea6bb94ec89af5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "prfA" ], "truncated": "3-prime", "pseudogene": { "start": 1935, "stop": 2678, "observations": { "mutation": [ 2373 ], "stop": [ 2373 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRE0", "query_cov": 1.0, "subject_cov": 0.40668523676880225, "identity": 1.0, "score": 272.0, "evalue": 2.81e-89, "gene_start": 1, "gene_end": 146, "reference_start": 1, "reference_end": 146, "reference_sequence": "MEYNKKLYEAIERVAIKNDALKKELETVVTDFKKIKEINIQLKKTTKIAEAFAKYKQKLDTGIAAEKILNTEKDLELIELAQMDLDEAKINIPIIENDLKIMLLPTDPNDDKNVIVEMRPAAGGDESSIFVGNLFDTYRAYAENNNWKMKIIEMTPNAVGFSFISFMISGEEVYSRMKFESGVHRVQRVPATESKGRVHTSTITVAVLPEQDEVDVVINPTELRIDTYRASGAGGQHVNRTESAVRITHIPTGVVAACQEGKSQIENRETAMKMLRAKLWEAAQEQQNAEFANLRKNQVGTGDRSEKIRTYNYPQNRVTDHRINLTLNKLDQIMMGELDEIIDALIADEQTGLMANLDI" }, "paralog": false, "identity": 0.9959514170040485, "score": 476.0, "evalue": 8.79e-172, "description": "Internal stop codon at 2373. Nonsense mutation around 2373.", "psc": { "uniref90_id": "UniRef90_Q9PRE0", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_Q9PRE0", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRE0", "UniRef:UniRef50_Q9PRE0", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRE0" ], "product": "Peptide chain release factor 1" } }, "id": "GPKLONFDEH_43", "locus": "GCA000006625_00003" }, { "type": "cds", "sequence": "contig_1", "start": 2379, "stop": 2774, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03462.23", "PFAM:PF00472.26" ], "nt": "ATGAAAATTATAGAAATGACTCCGAATGCTGTTGGTTTTAGTTTTATTTCTTTTATGATTTCTGGAGAAGAAGTTTATTCACGAATGAAATTTGAGTCAGGAGTTCATCGAGTACAACGTGTACCTGCTACTGAATCTAAAGGAAGAGTACATACGTCAACAATTACAGTTGCTGTTCTTCCAGAACAAGATGAAGTTGATGTTGTAATTAATCCAACAGAATTAAGAATTGATACTTACCGTGCAAGTGGGGCTGGAGGACAGCATGTTAATAGAACAGAATCAGCTGTAAGAATTACGCATATTCCAACAGGGGTTGTTGCTGCATGTCAAGAGGGAAAATCACAAATTGAAAACCGAGAAACAGCTATGAAAATGTTAAGAGCTAAATTATGA", "aa": "MKIIEMTPNAVGFSFISFMISGEEVYSRMKFESGVHRVQRVPATESKGRVHTSTITVAVLPEQDEVDVVINPTELRIDTYRASGAGGQHVNRTESAVRITHIPTGVVAACQEGKSQIENRETAMKMLRAKL", "aa_hexdigest": "1a9e1ba3fd7fc1c6b33f27c0ba5afd1b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000E5B535F", "query_cov": 0.9083969465648855, "subject_cov": 0.8263888888888888, "identity": 0.58, "score": 150.0, "evalue": 2.46e-44, "valid": false, "product": "PCRF domain-containing protein", "uniref50_id": "UniRef50_A0A7S1SZ85" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03462.23", "name": "PCRF", "length": 57, "aa_cov": 0.42748091603053434, "hmm_cov": 1.0, "evalue": 1.6635204203774114e-23, "score": 79.05348205566406, "start": 2, "stop": 57 }, { "id": "PF00472.26", "name": "RF-1", "length": 69, "aa_cov": 0.5190839694656488, "hmm_cov": 1.0, "evalue": 4.188213559602578e-32, "score": 106.15489196777344, "start": 64, "stop": 131 } ], "seq_stats": { "molecular_weight": 14458.414399999989, "isoelectric_point": 7.940973854064941 }, "id": "GPKLONFDEH_44", "locus": "GCA000006625_00004" }, { "type": "cds", "sequence": "contig_1", "start": 3014, "stop": 3886, "strand": "+", "frame": 2, "gene": "hemK", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "db_xrefs": [ "COG:COG2890", "COG:J", "RefSeq:WP_006688499.1", "SO:0001217", "UniParc:UPI0000165FB4", "UniRef:UniRef100_A0A2C9DXZ5", "UniRef:UniRef50_B5ZAH7", "UniRef:UniRef90_A0A2C9DXZ5" ], "nt": "ATGACCTACCATCAATTAATTTTTCAAGCATACTCACTACTTGAAAAAAAATTACGTAATCCACAAGTAGCATTTCAGTTGCTGTACGGATTAGATAATAAAATTAACGATTCTTATAGTTTTTCAAATAACCGCTTAACAATTGTAAATTCTAATTTAGAATATACATATTTTAAACTATTAGATGAATTTATTAATGGAAAACCATTAGTGAGAATTTTAGGATATGGCTATTTTTGTGCAAAGCGCTTTTATGTTGATAAAAATGTTTTTGCTTTTCGTGTTGAAACTGAATTATTAATAGATGTTGTTAATAAAGTAATTCAACAATCCACTTATAAAATTAAAAATGTTATTGATGTTTGTTGTGGTAGTGGTGTTTTAGGGTTAAGTACAAAAATGAATTTTAATCAATTGAATGTTTCATTATTAGATATTTCTATTGATGCAATTAATAATTGCAAAAAAAATGCTAAATATCATAACTTAACAGATGTAAATTTTATTCATAAAAGTATGCAAGAATACTTTTTAAACACAAAAAAACGGTTTGATTTAATTATCTGTAATCCCCCATATATCAAAAGTAATTATGAATTAAATCGTGAAGTCTTAGATTATGACCCCATTAATGCACTTATTGATTTCGATCACAAAGATGGAATTAGTTTTTATTTATTTATAATAAATAACATTAAATCAATTGCTAATGAGAAATTCACTATTATTTTTGAAATTGGTTTTGATCAAAAAGAAATTTTAGAAAAAGTTATTAAAAAAAATGAATTTCCTCTTTTTTACTACTTTATAAATGATTATAATAATCTATGTAGAATTTTAATAATAAGTAATATTAAATATGAAAATTTATAG", "aa": "MTYHQLIFQAYSLLEKKLRNPQVAFQLLYGLDNKINDSYSFSNNRLTIVNSNLEYTYFKLLDEFINGKPLVRILGYGYFCAKRFYVDKNVFAFRVETELLIDVVNKVIQQSTYKIKNVIDVCCGSGVLGLSTKMNFNQLNVSLLDISIDAINNCKKNAKYHNLTDVNFIHKSMQEYFLNTKKRFDLIICNPPYIKSNYELNREVLDYDPINALIDFDHKDGISFYLFIINNIKSIANEKFTIIFEIGFDQKEILEKVIKKNEFPLFYYFINDYNNLCRILIISNIKYENL", "aa_hexdigest": "958d1714e732d299dbdb8603679da43a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165FB4", "ncbi_nrp_id": "WP_006688499.1", "uniref100_id": "UniRef100_A0A2C9DXZ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165FB4", "RefSeq:WP_006688499.1", "UniRef:UniRef100_A0A2C9DXZ5" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DXZ5", "uniref90_id": "UniRef90_A0A2C9DXZ5" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DXZ5", "gene": "HemK", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "uniref50_id": "UniRef50_B5ZAH7", "cog_id": "COG2890", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_B5ZAH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAH7" ], "product": "peptide chain release factor N(5)-glutamine methyltransferase" }, "genes": [ "hemK" ], "id": "GPKLONFDEH_45", "locus": "GCA000006625_00005" }, { "type": "cds", "sequence": "contig_1", "start": 3873, "stop": 4103, "strand": "+", "frame": 3, "gene": "tsaC", "product": "L-threonylcarbamoyladenylate synthase", "db_xrefs": [ "COG:COG0009", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A084F1C9", "UniRef:UniRef90_A0AAC9X6K8" ], "nt": "ATGAAAATTTATAGAATAACTAATTTAAATGTGATTTATGATGCTTTAATGGCCAATAAATGTGTTCTAATCCCTACTGATACTATCATTGGTTTATTAGCAAAAAATCAAGATGTAATTTATGAAATTAAGCAACGTGATCGAAATAAGAAAATCATCCGCTTTGTAGCCGATTACAAATTACTTGGTGATTTAACTGTAGAACAAAAGCAATTTTTAGATCTTTTTTGA", "aa": "MKIYRITNLNVIYDALMANKCVLIPTDTIIGLLAKNQDVIYEIKQRDRNKKIIRFVADYKLLGDLTVEQKQFLDLF", "aa_hexdigest": "c60cada1a38a2fb179544b07032b1b4e", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaC" ], "truncated": "3-prime", "pseudogene": { "start": 3873, "stop": 4406, "observations": { "mutation": [ 4101, 4344 ], "stop": [ 4101, 4344 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6K8", "query_cov": 1.0, "subject_cov": 0.40860215053763443, "identity": 0.934, "score": 142.0, "evalue": 1.48e-41, "gene_start": 1, "gene_end": 76, "reference_start": 1, "reference_end": 76, "reference_sequence": "MKIYRITNLNAIYDALVANKCVLIPTDTIIGLLAKNQDVIYEIKRRDRNKKIVRFVADYKLLGDLTVEQEQFLDLFWPGSVTVIKNGVSYRMPNSPHILKLIQKLGPLYCSSANISGEEPVKNHNEAIFKFGANSKLIYVEAQQQIGVPSTIVDIDKWEYVRRGANIEMVDMFIKELKYNNTKEKE" }, "paralog": false, "identity": 0.9265536723163842, "score": 332.0, "evalue": 5.07e-119, "description": "Internal stop codon at 4101, 4344. Nonsense mutation around 4101, 4344.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6K8", "gene": "TsaC", "product": "L-threonylcarbamoyladenylate synthase", "uniref50_id": "UniRef50_A0A084F1C9", "cog_id": "COG0009", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6K8", "UniRef:UniRef50_A0A084F1C9", "COG:COG0009", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1C9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1C9" ], "product": "L-threonylcarbamoyladenylate synthase" } }, "id": "GPKLONFDEH_46", "locus": "GCA000006625_00006" }, { "type": "cds", "sequence": "contig_1", "start": 4146, "stop": 4346, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAATAGCCCTTATATACTAAAATTAATTCAAAAGTTAGGTCCGTTATATTGTAGTAGTGCTAATATTTCAGGTGAAGAACCAATTAAAAATCATAATGAAGCAATTTTTAAATTTGGAGCAAATAGTAAACTAATTTATGTTGAAGCACAACAACAAATTGGTGTTCCATCAACTATTGTTGACATCGATAAATGA", "aa": "MPNSPYILKLIQKLGPLYCSSANISGEEPIKNHNEAIFKFGANSKLIYVEAQQQIGVPSTIVDIDK", "aa_hexdigest": "47826b765cb1b8f8bea52daade5f2eb6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7275.315000000001, "isoelectric_point": 6.531050300598144 }, "id": "GPKLONFDEH_47", "locus": "GCA000006625_00007" }, { "type": "cds", "sequence": "contig_1", "start": 4431, "stop": 4895, "strand": "+", "frame": 3, "gene": "rpiB", "product": "Galactoside O-acetyltransferase", "db_xrefs": [ "COG:COG0698", "COG:G", "EC:5.3.1.6", "KEGG:K01808", "RefSeq:WP_006688688.1", "SO:0001217", "UniParc:UPI00000C1B63", "UniRef:UniRef100_Q9PRD9", "UniRef:UniRef50_Q9PRD9", "UniRef:UniRef90_Q9PRD9" ], "nt": "ATGATAAAAAAAATTTATTTTGGTAATGATCATGCTGCTTATGAAATTAAAGATCAAATTATCACTCATTTAAAACAAAAAGGTTATCAAATTATTGATGAAGGTGCACAAGCCGCATTAGGTTCTGTTGATTATAGTACATATGCATTAAAAGTTGCTAATGACGTTGTTAATGATACTAAAAATGATAGCATAGGTATTTTATTATGTGGCACGGGAATAGGTATGAATATAGCAGCAAGCAAAGTGCATGGAACGCGGGTTGCGTTAGTGTATAACGAAAGCAGTGCAAAACTAGCCAAGGAGCATAATAATGCTAACATAATTACTATAGGAGCACGTGAAAATAGTTTAAACCAAATTCTTAAAATGATTGATGATTTTTTAACAAGTAAATTTTTAGGCGAGCGTCACCAAAAACGTTTAGATATAATTGCAAAATATGAAAAAGAGCAAAATAGTTAA", "aa": "MIKKIYFGNDHAAYEIKDQIITHLKQKGYQIIDEGAQAALGSVDYSTYALKVANDVVNDTKNDSIGILLCGTGIGMNIAASKVHGTRVALVYNESSAKLAKEHNNANIITIGARENSLNQILKMIDDFLTSKFLGERHQKRLDIIAKYEKEQNS", "aa_hexdigest": "c58132e15fdd61a474b7a3f3a2d8a6e4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1B63", "ncbi_nrp_id": "WP_006688688.1", "uniref100_id": "UniRef100_Q9PRD9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B63", "RefSeq:WP_006688688.1", "UniRef:UniRef100_Q9PRD9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRD9", "uniref90_id": "UniRef90_Q9PRD9" }, "psc": { "uniref90_id": "UniRef90_Q9PRD9", "gene": "RpiB", "product": "Galactoside O-acetyltransferase", "ec_ids": [ "5.3.1.6" ], "uniref50_id": "UniRef50_Q9PRD9", "cog_id": "COG0698", "cog_category": "G", "kegg_orthology_id": "K01808" }, "pscc": { "uniref50_id": "UniRef50_Q9PRD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD9" ], "product": "Galactoside O-acetyltransferase" }, "genes": [ "rpiB" ], "id": "GPKLONFDEH_48", "locus": "GCA000006625_00008" }, { "type": "cds", "sequence": "contig_1", "start": 4991, "stop": 5365, "strand": "+", "frame": 2, "gene": null, "product": "putative protein UU007", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q9PRD8", "UniRef:UniRef90_Q9PRD8" ], "nt": "ATGTTAAATATTTTAAATAATGTATCTAATTCTTCACTATATTCAGCCGCTTCAAATGCTACTGCGCAAACTGGTTCAAATTTGATCAATGATCTAGTTCCAGAAACATTAACCGCTTCAGGAATTAGTATCGCTATTTCTGTTTTTAGTGTTATAGGTACGATCGTTATTGCTTTGAGTGTATTGCCACAAACAATTAAAACTTTACGTGAAAAAGATACAGCTTCATTAAGTTTATTACTATTCTTGCTTAATGGTATTGCTACAGCCTTTTTAACTCTATACGGTATTGGACTTGTTACAGTTCATCCAAATTCATTTTCATTCTTAGTGGATATTAAAAACGGAATGTTCATTTATAATAGAGAAGAGTGA", "aa": "MLNILNNVSNSSLYSAASNATAQTGSNLINDLVPETLTASGISIAISVFSVIGTIVIALSVLPQTIKTLREKDTASLSLLLFLLNGIATAFLTLYGIGLVTVHPNSFSFLVDIKNGMFIYNREE", "aa_hexdigest": "67735307c07979c082ca8673a87818bf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 4991, "stop": 5551, "observations": { "mutation": [ 5363 ], "stop": [ 5363 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRD8", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 219.0, "evalue": 4.15e-71, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MLNILNNVSNSSLYSAASNATAQTGSNLINDLVPETLTASGISIAISVFSVIGTIVIALSVLPQTIKTLREKDTASLSLLLFLLNGIATAFLTLYGIGLVTVHPNSFSFLVDIKNGMFIYNREEWVAGYLICGIFLIMGEALCSVTSFIVLFCKVNNMIKAKKMGMSEEEYYEKQIKPFLKVKGAN" }, "paralog": false, "identity": 0.9946236559139785, "score": 353.0, "evalue": 1.86e-126, "description": "Internal stop codon at 5363. Nonsense mutation around 5363.", "psc": { "uniref90_id": "UniRef90_Q9PRD8", "product": "Uncharacterized protein UU007", "uniref50_id": "UniRef50_Q9PRD8", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRD8", "UniRef:UniRef50_Q9PRD8", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD8" ], "product": "Uncharacterized protein UU007" } }, "id": "GPKLONFDEH_49", "locus": "GCA000006625_00009" }, { "type": "cds", "sequence": "contig_1", "start": 5402, "stop": 5551, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGAAGCATTATGTTCAGTAACTTCATTTATTGTTCTATTCTGCAAAGTTAATAACATGATTAAAGCTAAAAAAATGGGAATGAGCGAAGAAGAATATTATGAAAAACAAATTAAACCATTCCTAAAAGTGAAAGGAGCTAACTAA", "aa": "MGEALCSVTSFIVLFCKVNNMIKAKKMGMSEEEYYEKQIKPFLKVKGAN", "aa_hexdigest": "8a1b0daa51d203c93f6cf16ae31a8441", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5577.646500000001, "isoelectric_point": 9.032230186462403 }, "id": "GPKLONFDEH_50", "locus": "GCA000006625_00010" }, { "type": "cds", "sequence": "contig_1", "start": 5554, "stop": 5685, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTCACTTCATTATTGCAAGATGGTATATATGAAGTTGGTAATGGTGCAATCGTTACTGACCAATCACCATATTTAGGAATTACACCTGATTATCAGGGTGCATATGGTTTCCCAACACACCCTTGA", "aa": "MNFTSLLQDGIYEVGNGAIVTDQSPYLGITPDYQGAYGFPTHP", "aa_hexdigest": "1b8e452754c6341c71c69dbfde599fe4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4649.062200000002, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_51", "locus": "GCA000006625_00011" }, { "type": "cds", "sequence": "contig_1", "start": 5730, "stop": 5990, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAGTATTCTAATTCAGTCATACCAGCGGCTTTAGCTTTAGCTTTATTTGCAATTTTTAAAGCAAACACAATTATACTTGCAATGCAAGATAGTGTTTCACTTAGAGCTACAAGAATAAATCCTCCAGGGTTTACAGAAACACCTAATCAAGCAAATATCGCTAATAATCCTAAACCAGCAATTGAAATAATTCACATACCAATAGCCAAATTTTCAGTTCTTTTTGAAATTAGTACTTTAATAACAGCAGGTAAATAA", "aa": "MQYSNSVIPAALALALFAIFKANTIILAMQDSVSLRATRINPPGFTETPNQANIANNPKPAIEIIHIPIAKFSVLFEISTLITAGK", "aa_hexdigest": "523f5912c69bf3b70bb2504281847995", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9234.738499999994, "isoelectric_point": 9.397636985778806 }, "id": "GPKLONFDEH_52", "locus": "GCA000006625_00012" }, { "type": "cds", "sequence": "contig_1", "start": 6175, "stop": 7080, "strand": "+", "frame": 1, "gene": "mgtE", "product": "magnesium transporter", "db_xrefs": [ "COG:COG2239", "COG:P", "GO:0015095", "GO:0015693", "GO:0016020", "SO:0001217", "UniRef:UniRef50_A0A084F1J3", "UniRef:UniRef90_Q9PRD6" ], "nt": "GTGGAAAATAAACAAAGTACATTAAATTTAAATAACAACACAAATATTAATCAAAATTTTCTAACACAAAATATTTTAAAAACTTACGAACATAAAGATATTACAGGACTTAAAAAATTACTAAAATCAAAACGGATTGATAAAATTGTTGAAAGTCTTGAAACTATTAATGATACACAAATTATTTTATTTGTATTAGTTGCCACAAAAGATGGAATTTGTGGTGATATTTTTAAATATTTAAATACTGAATTAAAATCAAAAATCATAAATGACGCAAGTTTACAGCAATTAAGAATCATTTTATTTGAACTTTATAATGATGATGTGATTATTTTAAAAAATGATTTTCCTATGTATACTAAAAAAATCTTACTTTCATTAGACAGCCAACAAAGAGCAGCTATTAAGCAATTATCTGAATTTGATGAAGATGAAGCTGGTTCAATCATCAATTCTGACTTTTTTACAATCAATCAAAATCTTTCTATCAAAGAAGCACTAATTGAAATAAAAAGAATGTATAATGACTTTGAACAAAGTAATATTATATATGTAGTAGATAATTATAATCGTTTAAAAGGATATGTAACAATTCATTCATTACTTTTTGCAGATTCTTTTGAATTGAAAATTTCTAATGTTATTCAAGAAGATGTTTTCTATGTTCGTAGTGATGAAGATATTGATGCTGTTTTAGATATTTTCAGAAAGTATCAAATTGAACAATTAGCAGTTGTTGATAAAGATAACCAATTAATTGGTTATATTAGTGATAATGATATTTTACCCGTAATTAATGCTGAAACAACTACTGATATTTATAAAATGTATGGGATTAGCGAATTAGATTTTCCTTACATTAAATCAAGTGTTTTTGTTTTATTTAAATCACGTCTATTATGA", "aa": "MENKQSTLNLNNNTNINQNFLTQNILKTYEHKDITGLKKLLKSKRIDKIVESLETINDTQIILFVLVATKDGICGDIFKYLNTELKSKIINDASLQQLRIILFELYNDDVIILKNDFPMYTKKILLSLDSQQRAAIKQLSEFDEDEAGSIINSDFFTINQNLSIKEALIEIKRMYNDFEQSNIIYVVDNYNRLKGYVTIHSLLFADSFELKISNVIQEDVFYVRSDEDIDAVLDIFRKYQIEQLAVVDKDNQLIGYISDNDILPVINAETTTDIYKMYGISELDFPYIKSSVFVLFKSRLL", "aa_hexdigest": "88aa72bf53caf8f0b46be4b30de05c60", "start_type": "GTG", "rbs_motif": "GGAGG", "genes": [ "mgtE" ], "truncated": "3-prime", "pseudogene": { "start": 6175, "stop": 7383, "observations": { "mutation": [ 7078 ], "stop": [ 7078 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRD6", "query_cov": 1.0, "subject_cov": 0.5574074074074075, "identity": 0.924, "score": 534.0, "evalue": 3.61e-187, "gene_start": 1, "gene_end": 301, "reference_start": 1, "reference_end": 301, "reference_sequence": "MKNKQNTSNLNNNTNINQNFLSQNILKAYEHKDIAGLKKLLKSKRIDKIVESLEAIDDTQIILFVLVATKDGICGEIFKYLNTELKSKIIDDASLQQLRIILFELYNDDVTILKNDFPMHTKKILLSLDSQQRAAIKQLSEFDEDEAGSIVNSDFFTINQSISVKEALIEIKRMYNDFEQSNIIYVVDDYNRLKGYVTIHSLLFADSFDLKISSVVQEDVFYVRSDEDIDAVLDIFRKYQIEQLAVVDKNDQLIGYISDNDILPVINTETTTDIYKMYGISELDFPYIKSSVFVLFKSRLLWLAVLMISATCTGFLIDKFQNVGQLVTAGLSTLVIVPIIPAMTGTSGNAGSQAAASVIRALSIGEITTKEYNKVLAKEFLVGALIGLVLAVINFARLIIYFAIIKPDLQQYNVLYNNITNNPHSQMIVGAIVSAGSSVALFFAIVISKLLGGILPLLATKLKIDPTIMSTPILSTLLDMVTTIILFGFGILFLLIIVDKVKVEELNNMRESIHAVKNINSLLPKQNFLLNNSNYLNHLA" }, "paralog": false, "identity": 0.927860696517413, "score": 723.0, "evalue": 5.69e-264, "description": "Internal stop codon at 7078. Nonsense mutation around 7078.", "psc": { "uniref90_id": "UniRef90_Q9PRD6", "gene": "mgtE", "product": "magnesium transporter", "uniref50_id": "UniRef50_A0A084F1J3", "cog_id": "COG2239", "cog_category": "P", "go_ids": [ "GO:0015095", "GO:0015693", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRD6", "UniRef:UniRef50_A0A084F1J3", "COG:COG2239", "COG:P", "GO:0015095", "GO:0015693", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1J3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1J3" ], "product": "Magnesium transporter MgtE" } }, "id": "GPKLONFDEH_53", "locus": "GCA000006625_00013" }, { "type": "cds", "sequence": "contig_1", "start": 7093, "stop": 7740, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCAGCTACATGTACTGGTTTTTTAATTGATAAATTTCAAGATGTAGGACAATTAGTCACAGCAGGTTTATCAACATTAGTTATCGTACCAATCATTCCAGCAATGACGGGAACAAGTGGAAATGCTGGATCACAAGCTGCCGCCTCTGTGATTCGTGCTTTATCAGTTGGTGAAATTACTTTAAAAGAATATAATAAAGTCCTTGCCAAAGAATTTTTGGTTGGTGCTTTAATTGGTTTAGTTTTAGCAATTATTAATTTTGCTCGTTTGATTATATATTTTGCTATTATTAAACCTGATTTACAACAATACAATGTTTTATACGAAAATATTACTAATAATCCACATACTAAAATGATTGTAGGAGCAATTGTTTCAGCTGGATCATCAGTCGCTTTGTTTTTTGCTATTGTAATTTCTAAATTGCTTGGTGGAACACTGCCACTTTTAGCAACAAAATTAAAAATTGATCCTACAATTATGTCAACACCAATTTTATCGACATTACTTGATATGGTTACAACTATTATCCTATTTGGTTTTGGTATTATATTTTTATTAATTATTGTTGATAAAGTTAAAGTCGAACAACTAAATAATATCCGCGAGCATATTCATGCTATTAAAAATGTTAATTCATGA", "aa": "MISATCTGFLIDKFQDVGQLVTAGLSTLVIVPIIPAMTGTSGNAGSQAAASVIRALSVGEITLKEYNKVLAKEFLVGALIGLVLAIINFARLIIYFAIIKPDLQQYNVLYENITNNPHTKMIVGAIVSAGSSVALFFAIVISKLLGGTLPLLATKLKIDPTIMSTPILSTLLDMVTTIILFGFGIIFLLIIVDKVKVEQLNNIREHIHAIKNVNS", "aa_hexdigest": "79e62f7196c9b2c48e1e908f66da3515", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23004.234500000028, "isoelectric_point": 9.015017127990724 }, "id": "GPKLONFDEH_54", "locus": "GCA000006625_00014" }, { "type": "cds", "sequence": "contig_1", "start": 7954, "stop": 8454, "strand": "+", "frame": 1, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180", "RefSeq:WP_006688636.1", "SO:0001217", "UniParc:UPI0000133B67", "UniRef:UniRef100_Q9PRD5", "UniRef:UniRef50_Q9PRD5", "UniRef:UniRef90_Q9PRD5" ], "nt": "ATGGCAAATGTTAGACCAAGTGTTGTTTTTAAACAACAAGAAGTCAACCATATGGCAGATATACTAAAAAATTCAAAATCTTTTATTGTTTTTGAATATCATGGATTAACTGCTGCTAACATTTTAGCGTTAAGAAATGTTTTACATTCATCAAATTCTAAATTATATGTTTTAAAAAATAATATTACAGCACGTGCTTTTGAAAAAGCTGGTGTTAGTGGATTTGAAAATCAATTAACAGGACCTAACGCAGTTGCTGTTGCAATGGACGATGAAATTGCTGCGATTAAAGCAGTAAATGATGTAGCTAAAGAATTTGATTTTGTCAAAATTAAAGGTGCATATCTTGAAAACAAATTTGCAGATACTCACAAAATCGATCAACTTGCTGCAATTCCTGGCCGTGAAGGTCTATACTCAATGCTACTTTCTTGCTTCACAGCACCATTACGTAATGTGATGTATGGTTTAAAAGCTGTAGCTGAGCAAAAAGGTGAATAA", "aa": "MANVRPSVVFKQQEVNHMADILKNSKSFIVFEYHGLTAANILALRNVLHSSNSKLYVLKNNITARAFEKAGVSGFENQLTGPNAVAVAMDDEIAAIKAVNDVAKEFDFVKIKGAYLENKFADTHKIDQLAAIPGREGLYSMLLSCFTAPLRNVMYGLKAVAEQKGE", "aa_hexdigest": "1637497bdbaf949a27840b3c816cf6b6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000133B67", "ncbi_nrp_id": "WP_006688636.1", "uniref100_id": "UniRef100_Q9PRD5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133B67", "RefSeq:WP_006688636.1", "UniRef:UniRef100_Q9PRD5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRD5", "uniref90_id": "UniRef90_Q9PRD5" }, "psc": { "uniref90_id": "UniRef90_Q9PRD5", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_Q9PRD5", "cog_id": "COG0244", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRD5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD5" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "GPKLONFDEH_55", "locus": "GCA000006625_00015" }, { "type": "cds", "sequence": "contig_1", "start": 8479, "stop": 8844, "strand": "+", "frame": 1, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_006688489.1", "SO:0001217", "UniParc:UPI00001341D0", "UniRef:UniRef100_Q9PRD4", "UniRef:UniRef50_Q9PRD4", "UniRef:UniRef90_Q9PRD4" ], "nt": "ATGTCAAAATTAACAATTGAACAATTTATTGCTGCGATTAAAGAAATGTCAATGCTAGAATTAAACGATTTGGTTAAAGCTATCGAAACTGAATTTGGTGTGTCTGCTGCTGCTCCTGTTGCCGCTGCTGCTGCTCCTGTTGCCGCTGAAGCTCCAACTGAAGTAACTATTAAATTAGTTGAAGCTGGTACTAATAAAGTTGGTGTAATTAAATTAATTCGTGAGATTACAGGATTAGGATTAATGGAAGCTAAAACTGCTGCTGAAACTGCAGGCAGTGTAATTAAAGAAGATGTTAAAACAGAAGAAGCTAACGAAATCAAAAAGAAATTTGATGAACTTGGTGCAAAAGTACAACTTGTTTAA", "aa": "MSKLTIEQFIAAIKEMSMLELNDLVKAIETEFGVSAAAPVAAAAAPVAAEAPTEVTIKLVEAGTNKVGVIKLIREITGLGLMEAKTAAETAGSVIKEDVKTEEANEIKKKFDELGAKVQLV", "aa_hexdigest": "098fdee1bb231bf865286496741d2ff6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00001341D0", "ncbi_nrp_id": "WP_006688489.1", "uniref100_id": "UniRef100_Q9PRD4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341D0", "RefSeq:WP_006688489.1", "UniRef:UniRef100_Q9PRD4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRD4", "uniref90_id": "UniRef90_Q9PRD4" }, "psc": { "uniref90_id": "UniRef90_Q9PRD4", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_Q9PRD4", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD4" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "GPKLONFDEH_56", "locus": "GCA000006625_00016" }, { "type": "cds", "sequence": "contig_1", "start": 10161, "stop": 10619, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGTTAAATAAAAAAGTTCTATTTTTAGCAATTGGTAGCGTTTTTGCTTTAGGTACAATTATTACTGTAGCAACATCATGTACACAAAAATCTACATTAAACTATAGTCAATTTTATTGGACTTCACCAACAAGTGATGATGATGAAGGTTTTCAAACAAAATATAAAAGTATGGCTAGTGATGGTAAACGAGCTTTATTAATGCCTGGTTATCAACATCCTGAAAGATTACAAAATGCTTTAGTAAATGATAAATTTGATCCTAATTTAATCGCTTTAATTTTAGATGGAGTTTATAATAATGACAATAAAGCTGAGTTTTATAAGGGAGCAGATCGTGTTGCTGATGTTTATTTTAAAGTTGATGAAGCCGCTTTTTTAGGAGGTATTGCTGCAGCTTATATGTTAAACTCAAATCAAGCAGTTTTTGGTGCAGATAATAAATTAACTTGA", "aa": "MKKLNKKVLFLAIGSVFALGTIITVATSCTQKSTLNYSQFYWTSPTSDDDEGFQTKYKSMASDGKRALLMPGYQHPERLQNALVNDKFDPNLIALILDGVYNNDNKAEFYKGADRVADVYFKVDEAAFLGGIAAAYMLNSNQAVFGADNKLT", "aa_hexdigest": "270c2e645a2451bb70b32c12aa027ea8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16784.86429999998, "isoelectric_point": 6.559412956237793 }, "id": "GPKLONFDEH_57", "locus": "GCA000006625_00017" }, { "type": "cds", "sequence": "contig_1", "start": 11219, "stop": 11350, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGCTAAGAACCCGATCCCACCAACCTCGCCTGCAAATGGAGAACGTGATGCATACATATATAATCCACCACCAATTCCAGCAATAAAACCAGAAATCGAAACAGCAATTCATTGGTATTTATAA", "aa": "MAKAKNPIPPTSPANGERDAYIYNPPPIPAIKPEIETAIHWYL", "aa_hexdigest": "94730e08232a40bf283c56f485bd36d9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4786.463699999999, "isoelectric_point": 6.523149681091309 }, "id": "GPKLONFDEH_58", "locus": "GCA000006625_00018" }, { "type": "cds", "sequence": "contig_1", "start": 11335, "stop": 11934, "strand": "-", "frame": 1, "gene": "nupQ", "product": "Ribose/galactose ABC transporter", "db_xrefs": [ "COG:COG1079", "COG:F", "KEGG:K23536", "SO:0001217", "UniRef:UniRef50_Q9PRD2", "UniRef:UniRef90_Q9PRD2" ], "nt": "ATGCCTTCATTATCATTAATTAACGATGCCTCTATTTACGCTGCTATTTTAATTCTAGGTGCGTTAAGTGGTTTCTTTTGTGAACGTGTTGGGATTGCTAATATTTCAATTAATGGTCAAATGATTATTGGTGCCTTAGTCTTTACATTATTTTCAACAATTATTTATAAATATATGGGAACAAAAACGGAAACAGAATATACATTTATTGTATGTTTAATTATTGCTGGAATATTGACTATTCCAACTTCATTATTATTTGGTTTTTTAACTATAAAATTAAAAACCAATCAAATTATTGCTGGAACAGCTATTAATTTATTAGCTTCAGGAGTAGCAACCTTTGTAACTTATCCTTTGGGCGATAAAATTGCTGACAAAACAAGTCTATCTTCTGATTATTTAGGATTAATGAAAATTGGTGATGGAATCAACACTATTTATATTGGAAGTATTTTAATTTTAATTGCTGTTGTTTTAATTACCATTGGTTTAATGATAATGATGAAAAAAACTCCATTTGGTTTAAGATTATATGCAATTGGTGAAAACCCAAATGCAGCAGATGCACAAGGCATTAATGTTTATAAATACCAATGA", "aa": "MPSLSLINDASIYAAILILGALSGFFCERVGIANISINGQMIIGALVFTLFSTIIYKYMGTKTETEYTFIVCLIIAGILTIPTSLLFGFLTIKLKTNQIIAGTAINLLASGVATFVTYPLGDKIADKTSLSSDYLGLMKIGDGINTIYIGSILILIAVVLITIGLMIMMKKTPFGLRLYAIGENPNAADAQGINVYKYQ", "aa_hexdigest": "b9764402833ba191976f1276b8c97d6c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "nupQ" ], "truncated": "5-prime", "pseudogene": { "start": 11032, "stop": 11934, "observations": { "mutation": [ 11202, 11337 ], "stop": [ 11202, 11337 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRD2", "query_cov": 1.0, "subject_cov": 0.6440129449838188, "identity": 0.985, "score": 365.0, "evalue": 1.16e-125, "gene_start": 1, "gene_end": 199, "reference_start": 1, "reference_end": 199, "reference_sequence": "MPSLSLINDASIYAAILILGALSGFFCERVGIANISINGQMIIGALVFTLFSTIIYKYMGTKTETEYTFIVCLIIAGILTIPTSLLFGFLTIKLKTNQVIAGTAINLLASGVATFVTYPLGDKIANKTSLSSDYLGLMKIGDGINTIYIGSILILIAVVLITIGLIIMMKKTPFGLRLYAIGENPNAADAQGINVYKYQWIAVSISGFIAGIGGGLYMYASRSPFGGEVGGIGFLALAILIAGAWRIPLIVVVSIAFAVITKTFSQIDKIPQEIGKLIPYVITLIALISFSKYSVAPKNVGIPFDKSKR" }, "paralog": false, "identity": 0.9766666666666667, "score": 555.0, "evalue": 3.53e-203, "description": "Internal stop codon at 11202, 11337. Nonsense mutation around 11202, 11337.", "psc": { "uniref90_id": "UniRef90_Q9PRD2", "gene": "NupQ", "product": "Ribose/galactose ABC transporter", "uniref50_id": "UniRef50_Q9PRD2", "cog_id": "COG1079", "cog_category": "F", "kegg_orthology_id": "K23536", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRD2", "UniRef:UniRef50_Q9PRD2", "COG:COG1079", "COG:F", "KEGG:K23536" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRD2" ], "product": "Ribose/galactose ABC transporter" } }, "id": "GPKLONFDEH_59", "locus": "GCA000006625_00019" }, { "type": "cds", "sequence": "contig_1", "start": 11997, "stop": 12743, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTTAAAAAACATTTAATTATTGAAAGAGCAACACTTGTTTATTATCCAGAAATTAACACAACAAGAAAAATTAAAAATAAACTAAAACATTTTGAAAACATAAGCGCTAAAAAACTAAGTCGTATTAAAGACAAAATTCATAATGAAGATTTTTTAATTAAAAATTTAGTGGCTAAAGAAATTAGTAAATATGCTCAATCAAAATCGTTTGATTATAATAAGCTAAAATATTTATTACAAGAATATGAAAATAACAATTCAGTGTTAACAACCGAGTTAGTTAATGAGTTATCTTTAGTTCTAAAAGATGAACAAGCACAATTAATGTTTAGTGAATTTAAACAGGCAAGTTTTGAGAATAATTTAGATCACATGATTGATTTAAAAAATCAAATTATGACATACATTAATTCTTTATTAGATTCTCGATTAGAACAATTGTCAATAACTTTAAAATCAAAGAATTTAGTTCATGATGAACAAATGGATAAGATTATTTTATTACTAAATCCAAATGATAAAATTCTTAATAAAATTATTATTTCGCGTAATAAACATTTAGAAAATTACACGAAAGAAATTACAAAATTAGAATTAAGAAATATTTCGCAAGATGATGAAATTAAAAAGAATACAAAACAACGCTCATCATCATACTTGACAAAACATTATGAAAAAACTAAACAACATATTAATAAAATTAATCTAGATCAAGAACAAAAATTATTATTAGAAGATTGA", "aa": "MQLKKHLIIERATLVYYPEINTTRKIKNKLKHFENISAKKLSRIKDKIHNEDFLIKNLVAKEISKYAQSKSFDYNKLKYLLQEYENNNSVLTTELVNELSLVLKDEQAQLMFSEFKQASFENNLDHMIDLKNQIMTYINSLLDSRLEQLSITLKSKNLVHDEQMDKIILLLNPNDKILNKIIISRNKHLENYTKEITKLELRNISQDDEIKKNTKQRSSSYLTKHYEKTKQHINKINLDQEQKLLLED", "aa_hexdigest": "2837a0d4742dbcae5988e39c05c6a6d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29505.681700000074, "isoelectric_point": 9.187212181091308 }, "id": "GPKLONFDEH_60", "locus": "GCA000006625_00020" }, { "type": "cds", "sequence": "contig_1", "start": 13041, "stop": 13319, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATTATACTTCAACAACAACAAATGCGATTGTTATTAGTCAAGCTGCTGATGCCTTACCAACCCAAGGATATGATGGAATTGCGATGGGATTAATTTCTCTAGCACATCCTTTAGCAACAATTCCTGTCTCATTCTTAATGGGATTATTACAACAATCTGCTGATAATTTAGGAGGAAGTTTTCCACAAGATTTATCTGGAATTATTATTTCATTTGTAATGTTAGGGGCAGCAATGTTTATTTTATTTGAACGAATAAGTCCCGTTTATTGA", "aa": "MVNYTSTTTNAIVISQAADALPTQGYDGIAMGLISLAHPLATIPVSFLMGLLQQSADNLGGSFPQDLSGIIISFVMLGAAMFILFERISPVY", "aa_hexdigest": "46117efb0bc601b61f565408b36e4289", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9680.175399999996, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_61", "locus": "GCA000006625_00021" }, { "type": "cds", "sequence": "contig_1", "start": 13464, "stop": 13646, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTATATTACGCGTTATGTTGTTTTTACTGCACAAAGTAAAATGTATAATCCAACACCAGATCAAGTTGGATCACGATCAATTCCTTTTGCTGATAATTATTCTTTATTTCATTTAGCATCTGGATATGGTTATTTATTTGCTTTATTGATCTTTATTTCTTTATCTATTATTATTTGA", "aa": "MFYITRYVVFTAQSKMYNPTPDQVGSRSIPFADNYSLFHLASGYGYLFALLIFISLSIII", "aa_hexdigest": "a6b0bddab2971659c7475cb9e5cd007a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6862.916399999996, "isoelectric_point": 8.084029197692871 }, "id": "GPKLONFDEH_62", "locus": "GCA000006625_00022" }, { "type": "cds", "sequence": "contig_1", "start": 13650, "stop": 13847, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTTCAGGTTTGATTATGTTATTAGTCGTAAAAAACATGCAAATTGCCAATATTAATATGCCTAATGTTTTAGGTCAATTTTTCTTATTAATCATTGCAATGTTAGCAGGAGCAATGTTTGCAGTTTTTATAGGAATTTTAAAAGTATTTTTAAAGATTAATGAAGTTGTTAGTTCAATTTTATTGAATTGA", "aa": "MLGSGLIMLLVVKNMQIANINMPNVLGQFFLLIIAMLAGAMFAVFIGILKVFLKINEVVSSILLN", "aa_hexdigest": "1b88a877773901de8b4c0f7c75d2489b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7064.784899999997, "isoelectric_point": 9.700251579284668 }, "id": "GPKLONFDEH_63", "locus": "GCA000006625_00023" }, { "type": "cds", "sequence": "contig_1", "start": 13959, "stop": 14144, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAAATTTAAAACTCTTTTAAGTTACGATAGCTTTCTTAAAGGTCAAGAGCGAAAAACAATCACTAAAAAATTCTTATCGACAGTTTTTGCCATTTTTGTAGCGATAGTAGTTTCAGCTATTTTGGTTGGAATATTAGGCTATAACATACGTGATTTCTTTATAAGATTATTTACTAAATGA", "aa": "MLKFKTLLSYDSFLKGQERKTITKKFLSTVFAIFVAIVVSAILVGILGYNIRDFFIRLFTK", "aa_hexdigest": "2a313d487e1ad3476f72e27e2206998f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7051.467199999999, "isoelectric_point": 10.288783454895018 }, "id": "GPKLONFDEH_64", "locus": "GCA000006625_00024" }, { "type": "cds", "sequence": "contig_1", "start": 14147, "stop": 15754, "strand": "-", "frame": 3, "gene": "nupO", "product": "Methylgalactoside permease ATP-binding protein", "db_xrefs": [ "COG:COG3845", "COG:F", "RefSeq:WP_006688670.1", "SO:0001217", "UniParc:UPI00000C1B68", "UniRef:UniRef100_Q9PRD0", "UniRef:UniRef50_P47365", "UniRef:UniRef90_Q9PRD0" ], "nt": "ATGGAAATTAAGACAAATCAATGTCCTTACGCCATAGAAATGCACGAGATTACAAAAACTTTCCTGAACGGGACTGTTATTGCTAATAAAGATGTTAATCTTTTTGTTAAAAAAAATGAAATTCATGCAATTATTGGTGAAAATGGAGCAGGTAAATCAACCTTAATGTCAATTTTATTTGGGATCTATAAACAAGATTCTGGATCAATTAAAATTAATGGTCGAATTGTCAATTTTAGTTCAGCTAAAGATGCTTCAAAAACCGGAATTGGAATGGTACATCAACATTTTAAATTAATTGACACCTTAAGTGTTTTAGATAATGTTATTTTAGGTTCTGAAGGTGCAACGCAACTAGGTATCATTAACCGTAAAAAAATTACTAAAGAACTTAAAAAAATCATTCAAGAATATGGATTAAATATCAATTTAAAAAGTAAAATTTCAAAAATTACAGTTGGTCAACAACAAAAAACGGAAATTTTAAAATTATTATATCGTGATATTGATATTTTAATTTTTGATGAACCAACTGCTGTTTTGAGTGAAGATGAAATTCAAGCATTTTTACAAATGCTAAAAGATTTTAAAGCAGCAGGCAAGACAATCATTATTATTACGCATAAACTAAATGAAATTAAAGAAGTTGCTGATTCTGCAACAGTTATTCGTCGCGGACATTATATTGATAGTTTTGATGTTAAACAAAAAACAATAGCTGAAATGGCTGAATTAATGGTTGGTCGAAAATTGGTTGAAGTTAAAAATATCGATCCAATCACAAGTAATGAAAAAGTTTTCGAAGTTCAAAATTTAGATATTCAATCAATTGTTAAAAAACAAAAAGATATTGTGAAAGTTAAATCATCACCAAAAGGTGATGATGTTTCATTGTTAAGTACTAAAAATAATAATTTTATTAATTTTAGTATCCGCAAGGGTGAAATTTTTGCTATTGCCGGTGTTGAAGGTAATGGTCAAAGTGAATTAGCGCAAATAATCTCTGGCTTATTAAAAGGAAATAAGGAAGCAAAAATTATGCTTGATAATCAAGAAATTGAACATGCAAGCATTAGAAAACGTTATCAGTTAGGATTATCACATGTACCAGAAGATCGTCATAAACATGGATTAGTTTTAGATGACACGATTGCTATGAATGCGATCTTACAACAAGTTGATGATAAACCATATAGTAATTTAGGATTTTTTAATAATAATGAAATTTCAAAACATGCAATTAATATTATTAAAAAATATGATGTTCGTGGTACAACACGTGGTACATCAGATGCACGAGGATTATCTGGTGGAAACCAACAAAAACTAATTATTGGTCGTGAATTTGAACGATCTCATAAATTAATTTTATTGGTTCAACCAACAAGAGGTTTAGATTTAGGTGCGATTGAATTTATTCATGAACAAACATTAGAAGAGAAACGAAAAGGTAATGCTATTTTATTAATTTCTTACGAATTAGACGAAATTTTATCACTTGCTGATACAATTGCTGTAATTCATAATGGATATTTCATTAGTGTTGGTGATCGTTTTTCAATGACAAGACAAAAAATTGGTGAATTAATGGCAGGTGAAAAAATATAA", "aa": "MEIKTNQCPYAIEMHEITKTFLNGTVIANKDVNLFVKKNEIHAIIGENGAGKSTLMSILFGIYKQDSGSIKINGRIVNFSSAKDASKTGIGMVHQHFKLIDTLSVLDNVILGSEGATQLGIINRKKITKELKKIIQEYGLNINLKSKISKITVGQQQKTEILKLLYRDIDILIFDEPTAVLSEDEIQAFLQMLKDFKAAGKTIIIITHKLNEIKEVADSATVIRRGHYIDSFDVKQKTIAEMAELMVGRKLVEVKNIDPITSNEKVFEVQNLDIQSIVKKQKDIVKVKSSPKGDDVSLLSTKNNNFINFSIRKGEIFAIAGVEGNGQSELAQIISGLLKGNKEAKIMLDNQEIEHASIRKRYQLGLSHVPEDRHKHGLVLDDTIAMNAILQQVDDKPYSNLGFFNNNEISKHAINIIKKYDVRGTTRGTSDARGLSGGNQQKLIIGREFERSHKLILLVQPTRGLDLGAIEFIHEQTLEEKRKGNAILLISYELDEILSLADTIAVIHNGYFISVGDRFSMTRQKIGELMAGEKI", "aa_hexdigest": "64fc131700809a1f1d080d1dd9eeaddc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1B68", "ncbi_nrp_id": "WP_006688670.1", "uniref100_id": "UniRef100_Q9PRD0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B68", "RefSeq:WP_006688670.1", "UniRef:UniRef100_Q9PRD0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRD0", "uniref90_id": "UniRef90_Q9PRD0" }, "psc": { "uniref90_id": "UniRef90_Q9PRD0", "gene": "NupO", "product": "Methylgalactoside permease ATP-binding protein", "uniref50_id": "UniRef50_P47365", "cog_id": "COG3845", "cog_category": "F" }, "pscc": { "uniref50_id": "UniRef50_P47365", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47365" ], "product": "Putative carbohydrate transport ATP-binding protein MG119" }, "genes": [ "nupO" ], "id": "GPKLONFDEH_65", "locus": "GCA000006625_00025" }, { "type": "cds", "sequence": "contig_1", "start": 16143, "stop": 16760, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTTGAACAAGTATTTGCTAGCAAATACGTTGCTGGTTCATTTAAACCAGATGAAGAAGGTGCAACAAACATTATCAATGATTTAATTACAAAAAAAGCTGATGTTATTTTACCTGTAGCTGGTCCACAAACAAACCTTGCAACTTCAATTGTTTCAAATGCAACAGATCCAAGTGTAATTATTGGTGTTGATACTGCACAAGAATTAGATGATGTAACAAACCGTAAAAGAATTACAAACAAAACTGTTAATGATGGAAAAACAATTTTATTCTCAATTGTTAAACGTGTTGATTTAGCAATGAAGGGAGCAATTGAAAATGCTTCAAAAGGTGCACAACTAACTAATGATATTAATAAAGATGCTTATAAATTAGGAACACATACTGAAGCAAGTTTAGATAAGAGCACATATGTAGATGATACTCCGCTTGTTGAATTATCAAATGCAGGTCGTGTTTATTTAGAACAAGCGGCTAAACTAGCTGGTTTAAAAGCAATTACATATGCTCAAATTGTTAATGTTATTCAAAATGAAGAATTATTTAAACTTCTTTCAACAAAAGGAACTACAAAATTAGAAGATCTTGCTACAAAAACAAGCGATGGATGA", "aa": "MNVEQVFASKYVAGSFKPDEEGATNIINDLITKKADVILPVAGPQTNLATSIVSNATDPSVIIGVDTAQELDDVTNRKRITNKTVNDGKTILFSIVKRVDLAMKGAIENASKGAQLTNDINKDAYKLGTHTEASLDKSTYVDDTPLVELSNAGRVYLEQAAKLAGLKAITYAQIVNVIQNEELFKLLSTKGTTKLEDLATKTSDG", "aa_hexdigest": "6e7b4fd16eff4a41be83f7d80204441e", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22001.639900000002, "isoelectric_point": 5.132754325866698 }, "id": "GPKLONFDEH_66", "locus": "GCA000006625_00026" }, { "type": "cds", "sequence": "contig_1", "start": 16899, "stop": 17384, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGCAAAATCAATAAAAAAATCTTGTTTGCATCACTTGCTGGAGTGGTTACATTAACTTCAGTTGCAGCTATTGCTGCATCATGTAATGATAGTAATAAAGATGATGGGAAAACAAATAAAGATGGAAATTATATTTCTAAATTGTCTTTAGAAGATTTTTATGCAAAACCAGCTGGAGATGATAGTTTAGGATATCACAGAACATATAATTCATTATACGATGATGGTGCTCGTATGTTAGGCTTGATTTCATTTAGTCACTCAATTCCAATTAAAGAATATTTTGGTTCAAGCAGCGATAAAAAAGATTTAAGTGCAGTTTTAATTGATGATAAATTTAGTGGAACAGTGGGTAAAGACCGTATTGCGTCTGTCTCATATCGTGTAGATCAAGCAGCATTTTTAACAGGTATTGCTGCGGCGTACTATTTAAATGCTAACCAAAAAACTTTTGCAGCAGATGGAAAACTAACTTGA", "aa": "MKKSKINKKILFASLAGVVTLTSVAAIAASCNDSNKDDGKTNKDGNYISKLSLEDFYAKPAGDDSLGYHRTYNSLYDDGARMLGLISFSHSIPIKEYFGSSSDKKDLSAVLIDDKFSGTVGKDRIASVSYRVDQAAFLTGIAAAYYLNANQKTFAADGKLT", "aa_hexdigest": "5238c7ff71855df8bc27f2d6d6540aa3", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17331.307099999984, "isoelectric_point": 8.821547508239746 }, "id": "GPKLONFDEH_67", "locus": "GCA000006625_00027" }, { "type": "cds", "sequence": "contig_1", "start": 17555, "stop": 18352, "strand": "-", "frame": 3, "gene": "tatD", "product": "putative metal-dependent hydrolase UU017", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.-.-", "GO:0004536", "GO:0005829", "GO:0046872", "KEGG:K03424", "RefSeq:WP_006688502.1", "SO:0001217", "UniParc:UPI0000139104", "UniRef:UniRef100_Q9PRC8", "UniRef:UniRef50_Q9PRC8", "UniRef:UniRef90_Q9PRC8" ], "nt": "ATGTTAGAAAAAATAAAAATTATTGATACTCATACTCATCCTAATATTGAACCATTAATTGAAGAATTTGATGACATTATCTTTAAATGTTATGAACAAGGTATTGGATTAAATATTGTTGGAGTAGATTTAAAAACTTCACAGACAGCCATTCAACAAGCTAAAAAATTTAAATTTTTGACTTGTAGTGTTGCAATCCATCCTAATGATTTACATAATATAGAAAATGATTTTGTTGCATTAGAAAAATTGATAGCAGAAAATATTGATTACATAAGTTGTATTGGTGAGTGTGGTTTAGACTATTATCATGAACAAAATTATGATCATGATTTTCAAAAACATTGGTTTAAAAAACATATTGAATTGGCTAAAAAGTATCATAAACCATTAATGTTACACATCCGTAATGCACACGATGACGCAATTGCCATCTTAAAAGAATTAGCTGTTCAAGATGTAATAATCCATTGTTTTACAGATAAAAAAGAGTATGCACAACAATATATTGAAATGGGTTATTATATTTCATATCCTGGTGTAATTACTTTTAAACCAACAAAAAATAATCAATTACATGAACTGTATGAGGCGATTAAAATAACCCCACTTGATAAGATTCTAGTAGAAACAGACGCCCCATATTTAACACCAGTTCCTAAACGAGGGCAAACTAACTATCCTTATTATGTATTATATACATCAGCATTTATTGCCAACTTATTAGGCATTACACATGAAAAAATGCAAAAAATCTTATTAGAAAATTCATTAAGAATTCTAAATTTTAAAAAATAA", "aa": "MLEKIKIIDTHTHPNIEPLIEEFDDIIFKCYEQGIGLNIVGVDLKTSQTAIQQAKKFKFLTCSVAIHPNDLHNIENDFVALEKLIAENIDYISCIGECGLDYYHEQNYDHDFQKHWFKKHIELAKKYHKPLMLHIRNAHDDAIAILKELAVQDVIIHCFTDKKEYAQQYIEMGYYISYPGVITFKPTKNNQLHELYEAIKITPLDKILVETDAPYLTPVPKRGQTNYPYYVLYTSAFIANLLGITHEKMQKILLENSLRILNFKK", "aa_hexdigest": "0f0eca39b24e68b9a515d191ebbb879e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0000139104", "ncbi_nrp_id": "WP_006688502.1", "uniref100_id": "UniRef100_Q9PRC8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139104", "RefSeq:WP_006688502.1", "UniRef:UniRef100_Q9PRC8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRC8", "uniref90_id": "UniRef90_Q9PRC8" }, "psc": { "uniref90_id": "UniRef90_Q9PRC8", "gene": "TatD", "product": "Uncharacterized metal-dependent hydrolase UU017", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_Q9PRC8", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424", "go_ids": [ "GO:0005829", "GO:0004536", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC8" ], "product": "Uncharacterized metal-dependent hydrolase UU017" }, "genes": [ "tatD" ], "id": "GPKLONFDEH_68", "locus": "GCA000006625_00028" }, { "type": "cds", "sequence": "contig_1", "start": 18354, "stop": 19670, "strand": "-", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "EC:3.6.-.-", "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872", "RefSeq:WP_006688774.1", "SO:0001217", "UniParc:UPI0000137467", "UniRef:UniRef100_Q9PRC7", "UniRef:UniRef50_Q9PRC7", "UniRef:UniRef90_Q9PRC7" ], "nt": "ATGTCAACAATTGTTGCTTTAGCAACCGCGCCTATGAATTGTGCCATTCACATTATTCGGGTTAGTGGTCCAAAAGCTTTTGAAATAATTAATAAAATTTCAACAATAAAAATAAAAAAAGAAACTTTTAAAATTTGGTATACAATTCTAAAAGATGATAATCAAATTTTAGATGAAGTTTTAATAAATACATTTGTAGCTCCAAAAACATTTACTGGAGAAGATTTAGTAGAAATTAATTGCCACGGAGGAATAGTGGTTGCTAATTTAATTATTAAAACATTAATTAAATATGGTTGTCAACCAGCACAACGTGGAGAATTTTCTCGCCGTGCTTTATTAAATAAAAAAATGGATTTATCAAAAATTGAAGCAATTAACAATTTGGTTAATGCTAAAAATGAATTAAGCGTTAAAGGTGTAATTGGCGCTCTATTAGGACGTGTTTCGCAATCAATTGCTGAATTTAGGCATGAGTTATTTATGATAATTGGGCAAATTGAAGTTAATATTGATTATCCTGAATACGATGATGTTGAACAAGTTGATGCTATTATTTTAAAACAACGTTTATTATCTTTAGATAAAAAAATTACAAAAATTATTGATCAATCAAAAAAATTTTTACCAATCAATAAAGGCATTAGGGTGTTAATAATTGGAAAACCAAATGTAGGTAAATCAACTCTATTAAACGCTTTATGCAATGAACAAAAAGCGATTGTTACAGATATTCCTGGGACAACACGTGATGTTATTGAATCCTCAATTAATATTGATAATATTACTTTAAATATCTTAGATACTGCAGGAATTCATTTAACAAATGATTTTGTTGAAAATTTAGGAATTAATAAAGCCAAAGCATTAATTGATAAAGTTGATTTAATTTTATATTTAATACCTGCAAACGAACAACAAGATTTGGAGTTATATGATTTAATTAAAAAACAAAAACATTTATTAGTTTATACAAAAAAAGATTTAGTTGATCAATACGATGATAAACAAATATATATTAATGCAAAAAATAATGATATTCAGTCATTGATTGATGAAATTAAAAAACTTTTTTATGTTCAAGAGTTTGACAATGCTAACATTGATGTTTTACAATCCCAACGTCAAATTGGTATTTTAGAAAATGTTCATTATTTAATTAATAACGCAATTCTTAATTTAGAAAAAGGGGATACACTTGATTTAATTGTCGCTGATTTAGAATTTTGCAATTTACGTTTAAATGAACTCTTAGGCATAAGTAGCGAATATGATTTTTTAGATGATTTATTTAAAAATTTTTGTATAGGTAAATAA", "aa": "MSTIVALATAPMNCAIHIIRVSGPKAFEIINKISTIKIKKETFKIWYTILKDDNQILDEVLINTFVAPKTFTGEDLVEINCHGGIVVANLIIKTLIKYGCQPAQRGEFSRRALLNKKMDLSKIEAINNLVNAKNELSVKGVIGALLGRVSQSIAEFRHELFMIIGQIEVNIDYPEYDDVEQVDAIILKQRLLSLDKKITKIIDQSKKFLPINKGIRVLIIGKPNVGKSTLLNALCNEQKAIVTDIPGTTRDVIESSINIDNITLNILDTAGIHLTNDFVENLGINKAKALIDKVDLILYLIPANEQQDLELYDLIKKQKHLLVYTKKDLVDQYDDKQIYINAKNNDIQSLIDEIKKLFYVQEFDNANIDVLQSQRQIGILENVHYLINNAILNLEKGDTLDLIVADLEFCNLRLNELLGISSEYDFLDDLFKNFCIGK", "aa_hexdigest": "9eb6fc52831024999b4e6c3ed851a25c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0000137467", "ncbi_nrp_id": "WP_006688774.1", "uniref100_id": "UniRef100_Q9PRC7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137467", "RefSeq:WP_006688774.1", "UniRef:UniRef100_Q9PRC7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRC7", "uniref90_id": "UniRef90_Q9PRC7" }, "psc": { "uniref90_id": "UniRef90_Q9PRC7", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "ec_ids": [ "3.6.-.-" ], "uniref50_id": "UniRef50_Q9PRC7", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC7" ], "product": "tRNA modification GTPase MnmE" }, "genes": [ "mnmE" ], "id": "GPKLONFDEH_69", "locus": "GCA000006625_00029" }, { "type": "cds", "sequence": "contig_1", "start": 19673, "stop": 20467, "strand": "-", "frame": 3, "gene": "dnaX", "product": "DNA polymerase III delta subunit", "db_xrefs": [ "COG:COG2812", "COG:L", "RefSeq:WP_006688710.1", "SO:0001217", "UniParc:UPI00000C1B6A", "UniRef:UniRef100_Q9PRC6", "UniRef:UniRef50_Q9PRC6", "UniRef:UniRef90_Q9PRC6" ], "nt": "ATGAAATACAGTTTTGCTAATTTATTAATTCAATCACCTAAAACCTCACTAGAATCAGGTATTGAAGAAATTATGTTAGCTTTTATTTATGAGAAAAATCATAAAGAACAATCATTTTATGTTACTAAAGTTAAAAATAAACAATACTTTGATTTAAAAATTTATGATGGACTATCAATGAAAAAAAGCGATGTAATTGATTTACAAAACACTTTTTTATGTGATGGAGTAGAAGATATTAATTTAAAATTTTATCTTATTAAAAATATTGACTTAGCATCAAAATATGTTTTAAATGCATTATTAAAATTTATTGAAGAACCGCCAAAAAATACAATTGCAATTTTTAGCACTAAAAATTTAAATCAAGTTTTAAAAACTATTAAAAGTCGTTGTCAGCTTTTTTATTTACCTACTAATCATGATTTATATAAAAAATTAATTAACCAAATTAATCAACCAATTGATCTCGTGGAATGCGACTTAATGTTTGATGATTTGGATGAGCTAAAAATTTTATTAGAAAACAAGGAAATCAATGATGTTTTAACTTATTATGGAAAATTAAGTGATTTAAGAAGTTTTGAAATATTAAATGATTTAAAAGAAATATTTAAAAATTTATCTACCTTACAAATTCATTATCTATTAAAATTAATCTTCATTAGAATTAATAATGTAAATAGCAAAGAAATTATTTTGGATTTAATGCGTGCGAATTTAAAAATTAATATTAATAAAAATAATTTGTTTACTATTATTTATACAATTATTAATAAAAATAAAGGTGATTAA", "aa": "MKYSFANLLIQSPKTSLESGIEEIMLAFIYEKNHKEQSFYVTKVKNKQYFDLKIYDGLSMKKSDVIDLQNTFLCDGVEDINLKFYLIKNIDLASKYVLNALLKFIEEPPKNTIAIFSTKNLNQVLKTIKSRCQLFYLPTNHDLYKKLINQINQPIDLVECDLMFDDLDELKILLENKEINDVLTYYGKLSDLRSFEILNDLKEIFKNLSTLQIHYLLKLIFIRINNVNSKEIILDLMRANLKININKNNLFTIIYTIINKNKGD", "aa_hexdigest": "93a0e601525ade37897dc06432aafa6f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1B6A", "ncbi_nrp_id": "WP_006688710.1", "uniref100_id": "UniRef100_Q9PRC6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B6A", "RefSeq:WP_006688710.1", "UniRef:UniRef100_Q9PRC6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRC6", "uniref90_id": "UniRef90_Q9PRC6" }, "psc": { "uniref90_id": "UniRef90_Q9PRC6", "gene": "DnaX", "product": "DNA polymerase III delta subunit", "uniref50_id": "UniRef50_Q9PRC6", "cog_id": "COG2812", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_Q9PRC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC6" ], "product": "DNA polymerase III delta subunit" }, "genes": [ "dnaX" ], "id": "GPKLONFDEH_70", "locus": "GCA000006625_00030" }, { "type": "cds", "sequence": "contig_1", "start": 20468, "stop": 21160, "strand": "-", "frame": 3, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "RefSeq:WP_006688582.1", "SO:0001217", "UniParc:UPI000012E114", "UniRef:UniRef100_Q9PRC5", "UniRef:UniRef50_Q9PRC5", "UniRef:UniRef90_Q9PRC5" ], "nt": "ATGATATTAACGAAAAATAGTAATGAAAAAAAACCGCTTAAAAAAGGATTATTTATTGTTTTTGAAGGAATTGATGGTGCAGGGAAAACTTCAATCTTAAAACAACTTTTAGAGGTTTTAAAAGAACCTAAACTAGTTAATAAAATTTTTTTAACGCGTGAACCTGGTGGAAAAAACAATAATGCAGCAGAGATGATACGTGAGTTTTTTTTAAAAAATTTAGAAGTTTTTGATCCGTTAACACTAGCTTATTTATATGCAAGCAGTCGTGCTGAACACGTAAAAAAAACAATCAATCCTCATTTAGAAAAAGATCATATTGTAATTTCAGATCGTTTTGTTCATTCTTCATATATTTATCAAGGTATTGTTCAAAATCAAAGTTTAGATGTTATTTACCAAATTAATCAACAAGCAATTGGTGAATTAGAAATAGATTATGTTTTTTATTTTGATGTTAATGTTAATAATGCATTAAATCGAATGAAAAATCGTTTTGACAATACAAATGCATTCGATTCACAAAATAAACAATTTTATGAAAAGTTATTAAAACAATACCCTAGTGTTTTTAAAGTTTATAATCAACCTAAAAAAATTATTTTTATTGATGCCAATAAAAATGAAAATGAAGTATTATGTGAGGTTAAAGAACAATTATTAAAAATTTTTAAAGAACATAAATATATATAA", "aa": "MILTKNSNEKKPLKKGLFIVFEGIDGAGKTSILKQLLEVLKEPKLVNKIFLTREPGGKNNNAAEMIREFFLKNLEVFDPLTLAYLYASSRAEHVKKTINPHLEKDHIVISDRFVHSSYIYQGIVQNQSLDVIYQINQQAIGELEIDYVFYFDVNVNNALNRMKNRFDNTNAFDSQNKQFYEKLLKQYPSVFKVYNQPKKIIFIDANKNENEVLCEVKEQLLKIFKEHKYI", "aa_hexdigest": "c0003d9cf5fd74eb39b89aaf2a4b4f23", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI000012E114", "ncbi_nrp_id": "WP_006688582.1", "uniref100_id": "UniRef100_Q9PRC5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E114", "RefSeq:WP_006688582.1", "UniRef:UniRef100_Q9PRC5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRC5", "uniref90_id": "UniRef90_Q9PRC5" }, "psc": { "uniref90_id": "UniRef90_Q9PRC5", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_Q9PRC5", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC5" ], "product": "Thymidylate kinase" }, "genes": [ "tmk" ], "id": "GPKLONFDEH_71", "locus": "GCA000006625_00031" }, { "type": "cds", "sequence": "contig_1", "start": 21141, "stop": 21731, "strand": "-", "frame": 2, "gene": "menH", "product": "Triacylglycerol lipase", "db_xrefs": [ "COG:COG0596", "COG:HR", "SO:0001217", "UniRef:UniRef50_Q9PRC4", "UniRef:UniRef90_Q9PRC4" ], "nt": "TTGTTAGGTCATTCATTAGGAGCGGGGATTGCTAGTTTGGTTAGTTTTATTATCCCCCAACGCATTGAAAAATTAATTCTTGTTTGTCCATATCATTATCAATATTTAAATCCGTTTTTAAATAAAAAATTATTTAATGCTTGGGTTTTATTTCCCAATCCATTTTTAAAATTTAAAACAGATGTCGTTTTAAAAAAATTATATATTGATTATCGTAATAATTATAAAACCTTAATAGAAACTCGTTGGGAGTCAATTTCAAGAGAATATCCACGAATTGCACGCGATGTCTTATTTTTATGTTTATCGTTATTAAATATTAAAATCAATCATGAATTAAAAATGGCACAGCGTAATTTAATAATGCCAACATTAATTATGGTTTCAAGACATGATCAATTAATTGATTTTAATTTAGCTATTAAAGTGTTTAAAAACAACAATAAAGTAAATCAATATATTTTTAATAATTCTGGTCATATTCCTTTTATTGAAGAACCAAAATTATTTACTAATATATTACTAAGTTTTTTAGAGGATAGATTTGTAGAACAAGAGGAAAATGATAACAATGATATTAACGAAAAATAG", "aa": "MLGHSLGAGIASLVSFIIPQRIEKLILVCPYHYQYLNPFLNKKLFNAWVLFPNPFLKFKTDVVLKKLYIDYRNNYKTLIETRWESISREYPRIARDVLFLCLSLLNIKINHELKMAQRNLIMPTLIMVSRHDQLIDFNLAIKVFKNNNKVNQYIFNNSGHIPFIEEPKLFTNILLSFLEDRFVEQEENDNNDINEK", "aa_hexdigest": "a39a94b4203f097355b929297f938f0d", "start_type": "TTG", "rbs_motif": null, "genes": [ "menH" ], "truncated": "3-prime", "pseudogene": { "start": 21141, "stop": 22010, "observations": { "mutation": [ 21764 ], "stop": [ 21764 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRC4", "query_cov": 1.0, "subject_cov": 0.6782006920415224, "identity": 0.995, "score": 384.0, "evalue": 1.78e-133, "gene_start": 1, "gene_end": 196, "reference_start": 94, "reference_end": 289, "reference_sequence": "MIMELIKTNTLNFYFEPSKSELKKGSIVFIHGLDASPHYFFLINKDLSDYDCYFVGLPAHGLTPINNKKDLNIKVFAELFINWINEIDLKEFHLLGHSLGAGIASLVSFIIPQRIEKLILVCPYHYQYLNPFLNKKLFNAWVLFPNPFLKFKTDVVLKKLYIDYRNNYKTLIETRWESISREYPRIARDVLFLCLSLLNIKINHELKMAQRNLIMPTLIMVSRHDQLIDFNLAIKVFKNNNKVNQYIFNNSGHIPFIEEPKLFTNILLSFLEDRFVEQEENDNNDINEK" }, "paralog": false, "identity": 0.9965397923875432, "score": 584.0, "evalue": 5.69e-215, "description": "Internal stop codon at 21764. Nonsense mutation around 21764.", "psc": { "uniref90_id": "UniRef90_Q9PRC4", "gene": "MenH", "product": "Triacylglycerol lipase", "uniref50_id": "UniRef50_Q9PRC4", "cog_id": "COG0596", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRC4", "UniRef:UniRef50_Q9PRC4", "COG:COG0596", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC4" ], "product": "Triacylglycerol lipase" } }, "id": "GPKLONFDEH_72", "locus": "GCA000006625_00032" }, { "type": "cds", "sequence": "contig_1", "start": 21762, "stop": 22010, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATGGAATTAATTAAGACTAACACACTTAATTTTTATTTTGAGCCATCTAAAAGCGAACTAAAAAAAGGTTCGATTGTTTTTATACACGGCCTTGATGCATCTCCACATTATTTTTTTTTAATTAATAAAGATTTATCTGATTATGATTGTTATTTTGTTGGATTACCTGCACATGGTTTAACACCTATTAATAACAAAAAAGATTTAAATATTAAAGTTTTTGCTGAGTTATTTATTAATTGA", "aa": "MIMELIKTNTLNFYFEPSKSELKKGSIVFIHGLDASPHYFFLINKDLSDYDCYFVGLPAHGLTPINNKKDLNIKVFAELFIN", "aa_hexdigest": "932181009e9fb87062aa1af959f1ebed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9443.868599999993, "isoelectric_point": 6.244752311706543 }, "id": "GPKLONFDEH_73", "locus": "GCA000006625_00033" }, { "type": "cds", "sequence": "contig_1", "start": 22110, "stop": 22928, "strand": "-", "frame": 2, "gene": "fepD", "product": "Ferrichrome ABC transporter", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_Q9PRC3", "UniRef:UniRef90_Q9PRC3" ], "nt": "TTGTTTTCTGGAATTGCATTAGGAATTAGTGGTTATATTCTTCAAAGATTAACAAAAAATCGTTTAGCAGATAGTTCTTTATTAGGAATGGGCAATATTAACTTAGTGATTTTAACAATACTTTTTTTAATATTTGATTTTGGGCAATTGCAAATCCAAAAACGAATGGAATATGTTCTACCATTTATTTATTTAATTGGGTCAACAATGATTTGTTTTTTTATTCATTTTTTATGCAATTCTTCTACTGGATACATTTTTAAAAGAATTATTGTTTCAGGTATTATTATTAATTTGATTACAATTGTTATTGCTCAGTCATTAAGAATTTTAATGAGTAAAGAATCAAGTATGTATTTAAAAACAATTTTATTAGGTAATATTGAATCACGCAGCGATTTTTGTTTTTATTTTTGTTTTGGAATGCTTACTATTAGTGTTATTTGGTTAATGTTAAATGCAACTAAATTTAAGATTATGGTAACAAATCAACAAATATCTGGACAGTTAGGCATTAATAATAAGTCCTTGACAATTCAAACTTTTATTTGCATTAGCTTATTAGTATCGGTAAGTTATAGTTTAAGTGGTAATGTTATTTTTGTTGGGATTGTTGCAGCTAATTTTTCTTTCAATTATGTAAAAAACAATATTTCTAATGGCATTATTAATGCTGGATTAATAGGCGGCATTGTATTATTGATTAGTTATATTTTTGTTGTTTTAATTTTAAATTTATCAACTGACCAGACTATTTTATTAGTGCCAATTATTTCAGGACCATATTTTTTATATCAAGTTATTTTATTGAAAAATTAA", "aa": "MFSGIALGISGYILQRLTKNRLADSSLLGMGNINLVILTILFLIFDFGQLQIQKRMEYVLPFIYLIGSTMICFFIHFLCNSSTGYIFKRIIVSGIIINLITIVIAQSLRILMSKESSMYLKTILLGNIESRSDFCFYFCFGMLTISVIWLMLNATKFKIMVTNQQISGQLGINNKSLTIQTFICISLLVSVSYSLSGNVIFVGIVAANFSFNYVKNNISNGIINAGLIGGIVLLISYIFVVLILNLSTDQTILLVPIISGPYFLYQVILLKN", "aa_hexdigest": "fd620f9f840d60df035b5eb3d3ac9b28", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PRC3", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.996, "score": 494.0, "evalue": 6.62e-175, "valid": true, "gene": "FepD", "product": "Ferrichrome ABC transporter", "uniref50_id": "UniRef50_Q9PRC3", "cog_id": "COG0609", "cog_category": "P" }, "pscc": { "uniref50_id": "UniRef50_Q9PRC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC3" ], "product": "Ferrichrome ABC transporter" }, "genes": [ "fepD" ], "id": "GPKLONFDEH_74", "locus": "GCA000006625_00034" }, { "type": "cds", "sequence": "contig_1", "start": 22947, "stop": 23132, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTATAAAAATTAAGCATGATCAAAATTTAAAACACCAATTTGAACAAAAATTATTTTTTCGTTTTAAAAAGAACAAATTTTTTCTTTCATTAAGTTTATTAATAATAACAATTATTTTAACGCTGATTGCTGTAGCTGGAGCCTCGCGTTTTATAAATGTTTTTAGTTATTTGAGCGATTGA", "aa": "MSIKIKHDQNLKHQFEQKLFFRFKKNKFFLSLSLLIITIILTLIAVAGASRFINVFSYLSD", "aa_hexdigest": "7794f2eca4d83913b3a1ffd7babad4fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7176.512999999997, "isoelectric_point": 10.214580345153806 }, "id": "GPKLONFDEH_75", "locus": "GCA000006625_00035" }, { "type": "cds", "sequence": "contig_1", "start": 23163, "stop": 23348, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCCATTTAGTTCGTTTACTTTTTAAAAGAACTAATTATTTATTTCAAATGATTATCGCCATTTTTCTAACTACTTTTTTATTAGAATTACTTGGAATAATTGTAGAATTTTTTTCAAAATTTAATATTGTTTTTTTAGTAGCAACAGTTTTTGCGATTCCAATGATTGTTTTGCTAAAATGA", "aa": "MPHLVRLLFKRTNYLFQMIIAIFLTTFLLELLGIIVEFFSKFNIVFLVATVFAIPMIVLLK", "aa_hexdigest": "47f6a8c1a55f84ed434469ee70f5ee6f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7161.876999999997, "isoelectric_point": 9.992035484313966 }, "id": "GPKLONFDEH_76", "locus": "GCA000006625_00036" }, { "type": "cds", "sequence": "contig_1", "start": 23427, "stop": 23969, "strand": "-", "frame": 2, "gene": null, "product": "Ferrichrome ABC transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC2", "UniRef:UniRef90_Q9PRC2" ], "nt": "TTGTTTTTTTATTCTCCAATTGTTTTATTAGTTTGTGGTGCAGGATTGGTTTGCAGTGGATTTAGTTTGCAATCTACAACGCGTAATGGTTTGGCAGGTCCATCAACATTAGGAATTTTTCCATTAGCAGCCTTAGGTAATATTTTGAGTCAGTTAATTACCAAAAGTTTTTCTAATATTTTTATTGGCTATGTTTTTGGAATTATTTTTAGCTTATTTGCTTTAGGAATCAATTTTTTATTTATTAGAATTTCACCACATAAGAAAACTTTTAAACCTATTATTTTTGGTTTTTCATTAGGTGCCATTATCACAGCTGTTAATATTTTAATTGCTAATTTTAATTCATCAATTGTCGCTTCCCCCATTCAATTATTAGGTAGTATTGGTGTTTACAATTCTATTGAGCGCTTTTATATTGGCACTCCAATAGTGTTTATAAGTACGATTATTTTGTTATGTTATGCTAAAAAAATCCAAATTTTAGATACGGATCATTATTTAGCTAAATCACTAGGAATCGATGTTAATAAAGTGTTTTGA", "aa": "MFFYSPIVLLVCGAGLVCSGFSLQSTTRNGLAGPSTLGIFPLAALGNILSQLITKSFSNIFIGYVFGIIFSLFALGINFLFIRISPHKKTFKPIIFGFSLGAIITAVNILIANFNSSIVASPIQLLGSIGVYNSIERFYIGTPIVFISTIILLCYAKKIQILDTDHYLAKSLGIDVNKVF", "aa_hexdigest": "351dd1df52d014bd5ac4049adff32a11", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 23133, "stop": 24158, "observations": { "mutation": [ 23165, 23429, 24017, 24098 ], "stop": [ 23165, 23429, 24017, 24098 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRC2", "query_cov": 1.0, "subject_cov": 0.5278592375366569, "identity": 0.9940000000000001, "score": 341.0, "evalue": 4.27e-116, "gene_start": 1, "gene_end": 180, "reference_start": 64, "reference_end": 243, "reference_sequence": "MKIKLKFENKLNFKKYLIYKWSVFVVLVLLTSFIFIFNIIYTNKTFIWESFYFQNASFEEVFLLFFYSPIVLLVCGAGLVCSGFSLQSTTRNGLAGPSTLGIFPLAALGNILSQLITKSFSNIFIGYVFGIIFSLFALGINFLFIRISPHKKTFKPIIFGFSLGAIITAVNILIANFNSSIVASPIQLLGSIGVYNSIERFYIGTPIVFISTIILLCYAKKIQILDTDHYLAKSLGIDVNKVFWITSTCSIFIAISTTFLIGGLSLIAIVMPHLVRLLFKRTNYLFQMIIAIFLTTFLLELLGIIVEFFSKFNIVFLVATVFAIPMIVLLKWELGWKVNKN" }, "paralog": false, "identity": 0.9882697947214076, "score": 642.0, "evalue": 2.09e-237, "description": "Internal stop codon at 23165, 23429, 24017, 24098. Nonsense mutation around 23165, 23429, 24017, 24098.", "psc": { "uniref90_id": "UniRef90_Q9PRC2", "product": "Ferrichrome ABC transporter", "uniref50_id": "UniRef50_Q9PRC2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRC2", "UniRef:UniRef50_Q9PRC2" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRC2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRC2" ], "product": "Ferrichrome ABC transporter" } }, "id": "GPKLONFDEH_77", "locus": "GCA000006625_00037" }, { "type": "cds", "sequence": "contig_1", "start": 24256, "stop": 24411, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATTATGATAAATTAGAAAAAATTAATAAGGCTCCTAATGATAGTGAAAAAGCGGAATTTGTTGATAAAAATTTTGCAATTTATATGGGCCCATCCCATTTATTAAACTCAATGAATAAATTAATTATGATTAAAAATACAGGATCTTGA", "aa": "MINYDKLEKINKAPNDSEKAEFVDKNFAIYMGPSHLLNSMNKLIMIKNTGS", "aa_hexdigest": "d78386c7d6063e86cb10e86368b4df75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5817.690299999999, "isoelectric_point": 8.029811286926268 }, "id": "GPKLONFDEH_78", "locus": "GCA000006625_00038" }, { "type": "cds", "sequence": "contig_1", "start": 24763, "stop": 25224, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCAATCATAAAGCAACTGTTTTAGTTATAGAAAATCGTAATGAATTAGTTAATAATAAAGAAAATTATAATAAATTTTCTATTCTAACACCTTCAGATTTTCCACTAATCTATTCTAATCCTTATACAGAAGTTCCAGGATTGGGATTGAATTTTCCAAAACCAATTAATAATGATATTAGTGATATTATTGATCGTTATCAATCAATTGGTTATAATGCATTAAGAACAAACCCGCTCGAATCAGTCTTAAAACAAACATCTTCATTTTATAAAACAGCTGATTATGTTTTTTATATGTATGATTCTAATTTATTTACTAATAATAAATACAAAACAGATGGTCAAAAACGTGAAGATTTATTTAAAGAAATTTTATCAAACAAAAATTTTTATGCTCATAAATTCTTACGCAATTCTCGTTCAAAAATTATTTTTATTGATCGTTCATTGTTTTGA", "aa": "MRNHKATVLVIENRNELVNNKENYNKFSILTPSDFPLIYSNPYTEVPGLGLNFPKPINNDISDIIDRYQSIGYNALRTNPLESVLKQTSSFYKTADYVFYMYDSNLFTNNKYKTDGQKREDLFKEILSNKNFYAHKFLRNSRSKIIFIDRSLF", "aa_hexdigest": "2ae4db60647d84a2da83956365a0a467", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18046.192900000002, "isoelectric_point": 9.299773979187012 }, "id": "GPKLONFDEH_79", "locus": "GCA000006625_00039" }, { "type": "cds", "sequence": "contig_1", "start": 25294, "stop": 25428, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATTTTTTTCGTGATCCTTATAAAATTCATTTAGAATTTGCTCGCCAATTAGATAATGTTTTAAATTTTAATGAATTAAAAAAACGTCTACCAAAACCAATGCAAAATGTACATTCATTTACTGAATGA", "aa": "MNNFFRDPYKIHLEFARQLDNVLNFNELKKRLPKPMQNVHSFTE", "aa_hexdigest": "321dfe0f6068a8cbd6ba7632850a5bf9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5421.177500000001, "isoelectric_point": 9.396541023254393 }, "id": "GPKLONFDEH_80", "locus": "GCA000006625_00040" }, { "type": "cds", "sequence": "contig_1", "start": 25453, "stop": 25587, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTAATAATGAAAATTAGTTTTAAATTTAGATTAATTAATGTAATTTCAATGCTTGGATTAAGTATTATTTTTACAACATTAAGTTCATGTAAAAATCTTCAACAACAAAAACCGTATGATACTAATTGA", "aa": "MVLIMKISFKFRLINVISMLGLSIIFTTLSSCKNLQQQKPYDTN", "aa_hexdigest": "f4b9af083612899ba6ff3732aee7b71b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5079.116400000003, "isoelectric_point": 9.864903831481932 }, "id": "GPKLONFDEH_81", "locus": "GCA000006625_00041" }, { "type": "cds", "sequence": "contig_1", "start": 25674, "stop": 25802, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTAAAATTTTTTCAATTAGAGATTATCGTTATGTTATGCCCTTATCAGGATTATTAGGTGCAATGATAACATCTTTAGGTTTAATACTATATCGTGAGACAATTTATATTAATTCATCGATTTGA", "aa": "MRKIFSIRDYRYVMPLSGLLGAMITSLGLILYRETIYINSSI", "aa_hexdigest": "04c56e621443a3607de131d51d150698", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4869.810099999999, "isoelectric_point": 9.815069770812986 }, "id": "GPKLONFDEH_82", "locus": "GCA000006625_00042" }, { "type": "cds", "sequence": "contig_1", "start": 26019, "stop": 26405, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAATTGCTGGATGCGTGACACAAGCACTAACTAAAAATCCATTAGCTGATTCAAGTACATTAGGTATTGTACAATCAAGTGTATTTATGATTGTAATTGCATTCTCATATAACATGATTTCTTTTGGAGAACTTTTTGGTTTTGCCATTATTGGTGGAATAATTGCTAGCATTATTTTATTGATTCTAGTTTTTGCAACAAGAAGCAGATTATCATATATTAAAATTACTTTAGCAGGATTAGCTATAGGAGTTTTTTTTAATACAATTTCTTATTTTATTCGTATTCAAAGTGCTAAAGGTTCAAGTATTAATTTTAAATATGTTTTAGGGGGTAGTGAAAATATTTATACAACTCATATTGATCCTTTTTTAACATTATGA", "aa": "MAIAGCVTQALTKNPLADSSTLGIVQSSVFMIVIAFSYNMISFGELFGFAIIGGIIASIILLILVFATRSRLSYIKITLAGLAIGVFFNTISYFIRIQSAKGSSINFKYVLGGSENIYTTHIDPFLTL", "aa_hexdigest": "fff48b5a385315d11331a35eacd17bcc", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13785.157299999973, "isoelectric_point": 9.250584602355957 }, "id": "GPKLONFDEH_83", "locus": "GCA000006625_00043" }, { "type": "cds", "sequence": "contig_1", "start": 26589, "stop": 26714, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATTATACCTTTCGTTTTAAACTATTTAAACAAATTAAAAATCAACAAGTTAAAAAATTAAAGCCACTCATTTTAATAGGAGCAACAGTTTTTTTAATTATTGCTATTTTTAGTATTTGA", "aa": "MQNYTFRFKLFKQIKNQQVKKLKPLILIGATVFLIIAIFSI", "aa_hexdigest": "8673c47d07bd900127bc08554dda3993", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4855.955599999998, "isoelectric_point": 10.751730918884277 }, "id": "GPKLONFDEH_84", "locus": "GCA000006625_00044" }, { "type": "cds", "sequence": "contig_1", "start": 27029, "stop": 27313, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAACGTCCAGATGTTTTAATTCTAGTAGATCCTGTTAATGATGTTAATGCTATTAAAGAAGCACGTAAATTAAACATTCCAGTAATTGCTTTAGCAAATACGAATGCTGATCCACAATTGATTGATTACATTATTCCAGTAAATAATTATTCAGTTAAATCAATTACTTTGATTTTAGGTGTGCTAGCAGATTCAATTGCTGAATTGCACGGTGAACCAACAAAAATTGTTGGACGACCAGATAGCGAAATTGTTTTACCTGAAACAAAATCAAATTGA", "aa": "MKQRPDVLILVDPVNDVNAIKEARKLNIPVIALANTNADPQLIDYIIPVNNYSVKSITLILGVLADSIAELHGEPTKIVGRPDSEIVLPETKSN", "aa_hexdigest": "9b4b263f85f3487695270e9222c0826d", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10236.748999999993, "isoelectric_point": 4.932169532775878 }, "id": "GPKLONFDEH_85", "locus": "GCA000006625_00045" }, { "type": "cds", "sequence": "contig_1", "start": 27485, "stop": 27697, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGTTACATCCATGCTAAACGTTCAAATAACCATGTAATTGATATTTTAAAAACAATTCTATTTTTAGATCGTGCATACAAATTCTTACAAGAGGTTTCTCAAAACGGGGGCACTGTGATGTTTGTGGGTACAAGAGGTCGTGTTGTTAAAGAATTAATTAAAGCAGAAGCAGAACGTACTAATTCATTTTATGTAACTCAAAGATGA", "aa": "MASYIHAKRSNNHVIDILKTILFLDRAYKFLQEVSQNGGTVMFVGTRGRVVKELIKAEAERTNSFYVTQR", "aa_hexdigest": "39770ab6624dc6e5c2dee050631add45", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8061.2216, "isoelectric_point": 9.9877161026001 }, "id": "GPKLONFDEH_86", "locus": "GCA000006625_00046" }, { "type": "cds", "sequence": "contig_1", "start": 27707, "stop": 27928, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCAAATGGAAAATTCGACGAAAAAAGTTGAATCGGTAGCTAACGTTGAAGTAGTTACTACTGAAAATGCAAAAGTAGAACAAAAACCTACATCACCGGCTGATGCAATTCAGCTTAAAGTCAATGCAAATTCAACTATAAAAAATCTTAAAACTTTAGTTTCAATTGTAAAATTAACTGAATCAGGTGCTCATATTGGGTTAAATCCTAAGAAATGA", "aa": "MSQMENSTKKVESVANVEVVTTENAKVEQKPTSPADAIQLKVNANSTIKNLKTLVSIVKLTESGAHIGLNPKK", "aa_hexdigest": "3e196d22ba640387d34dd4dc42cc75fc", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7831.9281999999985, "isoelectric_point": 9.39911975860596 }, "id": "GPKLONFDEH_87", "locus": "GCA000006625_00047" }, { "type": "cds", "sequence": "contig_1", "start": 28508, "stop": 28999, "strand": "-", "frame": 3, "gene": null, "product": "Iron ABC transporter substrate-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAK0", "UniRef:UniRef90_A0AAC9T040" ], "nt": "GTGAATAAAAAAATTAAAACAGTTTTCATTAGTAGTTTAATTGTTGGACTATTAGGTTTGATAACAACAAGTGGTGTTGCGTGCAGCTCATCAATAAAATCAAAACAAGAGAATGAAATTTATCCAACCATTAAAGGTTATGATCAACATGATTTTTCAAAGATTTTAGCTTCAGAATTTATTAAGATTATTAAAACTAATATTCAATTTTTGGCACCAAATAAAGATATTAAATATGAATTATATGATGCACGATTGAATGAGTTAGATGAAGATGTAATTGATCTAATAATTCGTTATCATTTTAAAGGTCAAACGCACACTTACAAAAAAATCTTAAAAGGTTTTAAGAATGCTAAACAATATCGTTTATTTTTAGAAAATAAAAAATATTTAGAAGATGAAAAAAACCATAATACTCTTTATTTAATGCCCATAGTTAATTCAAGTATTAACATTAGTAAAACAACAGTAGATGAAGCATTAAAATGA", "aa": "MNKKIKTVFISSLIVGLLGLITTSGVACSSSIKSKQENEIYPTIKGYDQHDFSKILASEFIKIIKTNIQFLAPNKDIKYELYDARLNELDEDVIDLIIRYHFKGQTHTYKKILKGFKNAKQYRLFLENKKYLEDEKNHNTLYLMPIVNSSINISKTTVDEALK", "aa_hexdigest": "bd94d71da206b4e2e5872e2317c2995f", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 28205, "stop": 28999, "observations": { "mutation": [ 28510 ], "stop": [ 28510 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T040", "query_cov": 1.0, "subject_cov": 0.5046439628482973, "identity": 1.0, "score": 317.0, "evalue": 3.1e-107, "gene_start": 1, "gene_end": 163, "reference_start": 1, "reference_end": 163, "reference_sequence": "MNKKIKTVFISSLIVGLLGLITTSGVACSSSIKSKQENEIYPTIKGYDQHDFSKILASEFIKIIKTNIQFLAPNKDIKYELYDARLNELDEDVIDLIIRYHFKGQTHTYKKILKGFKNAKQYRLFLENKKYLEDEKNHNTLYLMPIVNSSINISKTTVDEALKWQNSYLNINNSNNDLKYEILKITKSKTANSIDIEIKISKGNGENLASITYNKTLNGFISEADKKIYLANLEAIKQDKNQLEPQLIDEKTKQKTIKEVIENYKTLFKLPFQTGFIYEIQSVEPDVNNKTAVVFKILIKKGNGSQEAVLEYLKTIKGFNQNK" }, "paralog": false, "identity": 0.9924242424242424, "score": 512.0, "evalue": 2.32e-186, "description": "Internal stop codon at 28510. Nonsense mutation around 28510.", "psc": { "uniref90_id": "UniRef90_A0AAC9T040", "product": "Iron ABC transporter substrate-binding protein", "uniref50_id": "UniRef50_B5ZAK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T040", "UniRef:UniRef50_B5ZAK0" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZAK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAK0" ], "product": "Putative ABC substrate-binding protein-iron" } }, "id": "GPKLONFDEH_88", "locus": "GCA000006625_00048" }, { "type": "cds", "sequence": "contig_1", "start": 29014, "stop": 29760, "strand": "-", "frame": 1, "gene": null, "product": "Iron ABC transporter substrate-binding protein", "db_xrefs": [ "RefSeq:WP_006688534.1", "SO:0001217", "UniParc:UPI00000C1B71", "UniRef:UniRef100_A0AAC9T360", "UniRef:UniRef50_A0AAC9T360", "UniRef:UniRef90_A0AAC9T360" ], "nt": "ATGAAAATAAAATTAAAAAAAATAATCATGTCTTTTTTGGGATTGCCAATTTTGGCAATTCCCCTTATCGTTAGCGCTTGTTCGCAATTTAAAATTAACCAGGATGTTATTTTAACTTATCGTCCAACAGTTTCATTAGCAAAAGAATTTGCTAATGATAACAATACTATTGATGATGTTTTAAAAAAAGTAAAAATCAATAAAGACGGATCAAATAACCTACTAGTTTATGATTTATCAAGAAATCCAAAGAATGTCCAGTATAAAATTGTTGATATTAAAAAGGATTCAGAACAAATTCTTAAAGTTACGATTAATGCTAAAATTCAAAAATATAAAGAATCAATTAATTATGATTTTTATTTTCAACCATTTTTAACTCCTAAACAAAAAGAAGATAAAACAATCCTTCAACAAAATTTAAATATTATTAAAAGTGCTTGGGATTATGATCAAAAACAAAAAATAGGTTTTTTTAATCTTAGAATAAAACCAGAATATCAAAAAAGAAGTTTGGTTGAAATTAAAACATTAGGTGAAAAAGCTTTTAATTTTGGTGAGGATTTAATTCCTCAATTAAAAGTAGACTCAAAATTTAAAGATATTAATATTAAAATAATAGACATTAACTATTTTGAACCATTAGATGAAAGACAACGTGAATTAGTTGTGGAAATAATGATTAGTAAAGGTAAAAATGAACAACAGGCTCAATTATTTAAAAAAATTAAAATTAATTTAGACTAA", "aa": "MKIKLKKIIMSFLGLPILAIPLIVSACSQFKINQDVILTYRPTVSLAKEFANDNNTIDDVLKKVKINKDGSNNLLVYDLSRNPKNVQYKIVDIKKDSEQILKVTINAKIQKYKESINYDFYFQPFLTPKQKEDKTILQQNLNIIKSAWDYDQKQKIGFFNLRIKPEYQKRSLVEIKTLGEKAFNFGEDLIPQLKVDSKFKDINIKIIDINYFEPLDERQRELVVEIMISKGKNEQQAQLFKKIKINLD", "aa_hexdigest": "684f7ff4d7e539a9270917291964e359", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1B71", "ncbi_nrp_id": "WP_006688534.1", "uniref100_id": "UniRef100_A0AAC9T360", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B71", "RefSeq:WP_006688534.1", "UniRef:UniRef100_A0AAC9T360" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T360", "uniref90_id": "UniRef90_A0AAC9T360" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T360", "product": "Iron ABC transporter substrate-binding protein", "uniref50_id": "UniRef50_A0AAC9T360" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T360" ], "product": "Iron ABC transporter substrate-binding protein" }, "genes": [], "id": "GPKLONFDEH_89", "locus": "GCA000006625_00049" }, { "type": "cds", "sequence": "contig_1", "start": 29766, "stop": 31886, "strand": "-", "frame": 2, "gene": "tex", "product": "S1 motif domain-containing protein", "db_xrefs": [ "COG:COG2183", "COG:K", "KEGG:K06959", "RefSeq:WP_010891652.1", "SO:0001217", "UniParc:UPI00000C1B72", "UniRef:UniRef100_Q9PRB6", "UniRef:UniRef50_Q9PRB6", "UniRef:UniRef90_Q9PRB6" ], "nt": "ATGATTGATTTAATTATTAAAACACTAGTTCAAAAATTAAAAATTGAGGCAAAATATATAACAAATGTTTTGAATTTATTAGCTGATAATAATACTATTGCATTCATTGCTAGATATCGTAAACATTTGACTAACAATATGGATGAATTTCAAATTCAAGCAATTGCACATGAATATGAATATCTTACAAAATTAAATAAACGTAAAGAAGTAATTATTAAAAATTTAGAACAAAAAGGTTTATTAACTAATGACTTATTAGAATTAATTAATAATTGTCAACGGCTTGTTGATCTTGAAAATCTTTATGAACCTTATGCCTCAAATAAAAAAACAAAAGCATCAATCGCCATTGAAAAAGGGTTAGAACCATTAGCATTATTAATATTAAAAAATAATCCTAATTCTAATATTAAAGAAGTTGCAAAACAATATTTAAATGAACAAGTTTTAAGTGTTGATGAAGCTATTGCTGGAGCTTGTGATATTATTGCCCAAAAAGTTGCTAACGATACAACTTTAAGAGAAATGCTTTATGAAACAATTAACAAACATGCTAAATTAATCACAAGAATTAATAAAATAACAAATGACCCAACTAAAAACTTTGCTTTATATTATGAGTTTAGTTGCCCAATTAAATATTTAAAAGCATATCAATTAATGGCTATTGATCGAGCTAATGAATTAAAAATCATTGCATTTAAACTTGATTTTAAAAAAGAATTTCTTATTGATTTTGCTGTCAATAAATATACTAGAAAAATCAAAAGTAACAGCTATGACTACATTAAATCAGCTGTTAATGATGGTTTTGATCGTTTATTAATTCCTTCAGTTTCAAATGCTGTTTACAAAGAAAAATTAGAGGAAGCTCACCAACAATCAGCACAAATTTTTAGTAATAATTTACAACAATTATTATTACAAAAACCTTTAAAAAGCCATGTTGTTTTAGGATTTGATCCAGGATATGTGCATGGTTGCAAATTAGCAATTGTTAATAAAAACAATCAATTATTACATACTGATATTATTTACCCTCACAAACCACAGATATTAATTACACAAGCTAAAAATACGTTAATATCCTTAATTAATAAATACGAAGTTAATACAATAGCAATTGGCAATGGAACAGCATCAAATGAAAGTGTAATTTTTATTAGTGATTTAATTAAGGAATTCAAATTAAATCTTAATTATTGTGTAATTAGTGAAGATGGTGCTTCAATTTATTCAGCATCATCAATTGCAAGTGAAGAATTTCCTAACTTAAGTGTTGAAAAACGTTCGGCAATTAGTATTGCACGAAGAATCATTGATCCTTTAAGTGAATTAATAAAAATCGATCCAAAAAGTATTGGTGTAGGTCAATATCAACACGATATTAATAAAAACATTCTTCAACAAAAAGTTGATTTTTGTATTGATTATTGTGTTAATCAAGTTGGTGTTGATGTTAATACTGCTTCAATTCCTTTGTTGTCTAAAGTATCAGGTTTAAATAAGCGAAGTGCAAAAAAAATTTTTGAATATGTTAAAAAACATCAATTTATTGAAAATCGTGAACAATTAAAAACCATTCCTTACATTACCGATAAAGTTTTTGAGCAAGCCGCTGGTTTTTTACGAATTAATAATGCTAATAATTTTTTAGATAGAACAAGTATTCATCCAGAAGCATATCTAGTTGTTAATGAATTATGTAAATATTTACAATTACCAATTAATAAATTAATAAATAATTATCAAGTTTTAAATCAATTAAATCCTAATGAATTAACTACTATTCTAAAAACAGATATTTATACTATTGAAAACATTATTAATAACTTAAAAAATCCTTTACAAGATGTTCGTGATGATTATGATATTCCTATTTTACGTTCACAAATGGTTGATATTAATAACTTGAAAATAGGGATGTTAGTACAAGGAACAATTCGTAACCAAACAGAGTTTGGTAGTTTTATTGATATTGGTTTAAAAAATGATGCATTGTTACATATTTCAAACTATTGTGAAGAAGATAATTTGCATGTTAATAAAAATATTAATGTTTATATTGATAAAATAGATACAATTACTCAAAAAATTTCATTAAAGTTAAAATTATAG", "aa": "MIDLIIKTLVQKLKIEAKYITNVLNLLADNNTIAFIARYRKHLTNNMDEFQIQAIAHEYEYLTKLNKRKEVIIKNLEQKGLLTNDLLELINNCQRLVDLENLYEPYASNKKTKASIAIEKGLEPLALLILKNNPNSNIKEVAKQYLNEQVLSVDEAIAGACDIIAQKVANDTTLREMLYETINKHAKLITRINKITNDPTKNFALYYEFSCPIKYLKAYQLMAIDRANELKIIAFKLDFKKEFLIDFAVNKYTRKIKSNSYDYIKSAVNDGFDRLLIPSVSNAVYKEKLEEAHQQSAQIFSNNLQQLLLQKPLKSHVVLGFDPGYVHGCKLAIVNKNNQLLHTDIIYPHKPQILITQAKNTLISLINKYEVNTIAIGNGTASNESVIFISDLIKEFKLNLNYCVISEDGASIYSASSIASEEFPNLSVEKRSAISIARRIIDPLSELIKIDPKSIGVGQYQHDINKNILQQKVDFCIDYCVNQVGVDVNTASIPLLSKVSGLNKRSAKKIFEYVKKHQFIENREQLKTIPYITDKVFEQAAGFLRINNANNFLDRTSIHPEAYLVVNELCKYLQLPINKLINNYQVLNQLNPNELTTILKTDIYTIENIINNLKNPLQDVRDDYDIPILRSQMVDINNLKIGMLVQGTIRNQTEFGSFIDIGLKNDALLHISNYCEEDNLHVNKNINVYIDKIDTITQKISLKLKL", "aa_hexdigest": "342e7d64d2bf3c646922b510b16d72d5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1B72", "ncbi_nrp_id": "WP_010891652.1", "uniref100_id": "UniRef100_Q9PRB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B72", "RefSeq:WP_010891652.1", "UniRef:UniRef100_Q9PRB6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRB6", "uniref90_id": "UniRef90_Q9PRB6" }, "psc": { "uniref90_id": "UniRef90_Q9PRB6", "gene": "Tex", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_Q9PRB6", "cog_id": "COG2183", "cog_category": "K", "kegg_orthology_id": "K06959" }, "pscc": { "uniref50_id": "UniRef50_Q9PRB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRB6" ], "product": "S1 motif domain-containing protein" }, "genes": [ "tex" ], "id": "GPKLONFDEH_90", "locus": "GCA000006625_00050" }, { "type": "cds", "sequence": "contig_1", "start": 31996, "stop": 32301, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCTAAAAACAAAATTTTTTTTAAAAGTTATAAGTGTTATTGCTCCAATTGTTATAATTCCAACTATTTTAGCTAATTGTGCACATATAAATTCTAATGAACTTGATAATGCTAAAACAAACTTGAAAGGAAGTGTTGAAGTTGTTAATATTAATCCTTATAAAACAAAACAAATGTTAGCATCAAATATCAACAAAGAACAAATTAATTCTTATTTTATTTTTATTTTTAATTTAATTAAAACAAATCAAAAAATTGAAGAAAAAATTTTAGATAAAAATGATTATGAAATTCTTAATTGA", "aa": "MNLKTKFFLKVISVIAPIVIIPTILANCAHINSNELDNAKTNLKGSVEVVNINPYKTKQMLASNINKEQINSYFIFIFNLIKTNQKIEEKILDKNDYEILN", "aa_hexdigest": "6957b119cb88a9b3b88784fc2ec65a1d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11568.43689999999, "isoelectric_point": 9.041320228576659 }, "id": "GPKLONFDEH_91", "locus": "GCA000006625_00051" }, { "type": "cds", "sequence": "contig_1", "start": 32530, "stop": 33153, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTGCAAAAATTTATTTTAATCAAAATGAAGCTAATGTTGATGATTCTAAACTTTATATGAACAAAATCGGTTATTCTGAATTTGGTAGTGATGTCCAAAAAAGATTAGAAAACGCCTTTAAAATTCGTTATGATGAGCAAAATATTTATCAAATTAATTCTCCGCAAATTTTAGCTAAAGAAACAAAAACGCTTACATCTTATATTGATAAAAAAACAAACAACAATTATAGTATTAATATAGAATTAATCAAAAAATTAATCCAAATTAATCCTTTTGGTAAACTCCCCAAAAATTTCGCTCAACTAATTAATTTAATTAAAAAAGAAGAATATCCTAAATTCTTGACAATTACTAAAAACGAATCTGTTGACAATATTGTTGTTAAAGATATTTATTATCGGATTATTGATCGTTATGCAAAATTAGAATTTATTTTAGAAATTTATAACAAAAAAACAAAACAAACCGTATATTTAAGTGCTAATTTTAATCAAAAGAATTCAGGATTATTAAAAAATGAAGATTATTTTCAATATATTTTTGATCGTACCATCTCACTTGATTTACTAACAACAAAAGATGGAAAAAATGTTGAATTAAATTCTGGAACTGGTTGA", "aa": "MRAKIYFNQNEANVDDSKLYMNKIGYSEFGSDVQKRLENAFKIRYDEQNIYQINSPQILAKETKTLTSYIDKKTNNNYSINIELIKKLIQINPFGKLPKNFAQLINLIKKEEYPKFLTITKNESVDNIVVKDIYYRIIDRYAKLEFILEIYNKKTKQTVYLSANFNQKNSGLLKNEDYFQYIFDRTISLDLLTTKDGKNVELNSGTG", "aa_hexdigest": "10e8328bdc3d5f0a3b5d00cb60bc4a1c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24337.518400000015, "isoelectric_point": 9.233758354187014 }, "id": "GPKLONFDEH_92", "locus": "GCA000006625_00052" }, { "type": "cds", "sequence": "contig_1", "start": 33268, "stop": 33501, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCACGCTGGTTTAATAAACAAGAATATATAAATTATTTAGAAAATAACGCTGGTTTCATATCCTCAAACATTTATGAAGATAAAGACCGTTATCAATATTTACTATGGGGGACTGCGCCTCTAAAATCTCCAGTAAGTAATAAATATAATTCATTAAGTGGTATTAGTTTTTCAAATTTAGCAAAGGTTTATAATATTACAAATCAAAATTTCATTAATCGAGCATGA", "aa": "MKSRWFNKQEYINYLENNAGFISSNIYEDKDRYQYLLWGTAPLKSPVSNKYNSLSGISFSNLAKVYNITNQNFINRA", "aa_hexdigest": "3b7a31f131f8f381b6cb0ae2b1466fae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9053.017799999994, "isoelectric_point": 9.476095008850098 }, "id": "GPKLONFDEH_93", "locus": "GCA000006625_00053" }, { "type": "cds", "sequence": "contig_1", "start": 33736, "stop": 34239, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGCCACAATTACAACTATTTAGTGGAGGATATCCTGGTGATGTTAATCCTAATAGTTCAGCGATTGTTTCATGGCGCGGTTCTAAATCTTATGGTTCACTAATTCAAGCATTTGATCGTGAAATTAAAAATGAATCAATTTTAGATTATTATGGTCCAAAGCAAATTAATAATATTGATGGTTATCAAAAGGTTGGCGAAGGTTATTTAAATAAATTATTTAATGTTGGAACACGAGTTATTACCTCTGATGAAATAGGTGATTTGGGTTCTGGTTCATCTGGTTCAATGATTATTGATTCTAACTTTAATTTAGTAGGTATTCATTTTGCTTCTTTAAATTCGCGTGCTTATGGTGCGCCAAATGATTCAATGATTGGTAATTTATTTGTTGCTCAATCCCAAGACTTAAGTGGTGATATTGATGTAAGAGCTGCGGTGATAAAAAAACTAAAAGCTGAAAATATTTACACTTATAAACTAAACCCTAAAGTTAGTTCTTAA", "aa": "MPQLQLFSGGYPGDVNPNSSAIVSWRGSKSYGSLIQAFDREIKNESILDYYGPKQINNIDGYQKVGEGYLNKLFNVGTRVITSDEIGDLGSGSSGSMIIDSNFNLVGIHFASLNSRAYGAPNDSMIGNLFVAQSQDLSGDIDVRAAVIKKLKAENIYTYKLNPKVSS", "aa_hexdigest": "c2727e491a7f0df65d6e63702d04caf6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 128, "aa_cov": 0.6287425149700598, "hmm_cov": 0.9609375, "evalue": 3.105274772708889e-08, "score": 29.20621681213379, "start": 7, "stop": 111 } ], "seq_stats": { "molecular_weight": 18096.051299999996, "isoelectric_point": 5.771169853210449 }, "id": "GPKLONFDEH_94", "locus": "GCA000006625_00054" }, { "type": "cds", "sequence": "contig_1", "start": 34901, "stop": 35191, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTTATTAATCAAAATTACTATTACATTAAACGTATTCATAATTTACTTATACCAATAAGAATTGATGAAATTAAAATTTTAGAATATAGTTACATTTATTTATTACGTTGTGGATTTTATATTTTATTAACAGTATTTATAGCAAAATTTATTCATGATAAAAGAAGTATGATAAAGGTTTTATCATTATATTTATTCGTTAATTTTATATTAATGATTATTGGAACTTCATTTACTTTTTTAGAACATACAAACTTTTATAATGGTATGTTAACTTATTGA", "aa": "MIVFINQNYYYIKRIHNLLIPIRIDEIKILEYSYIYLLRCGFYILLTVFIAKFIHDKRSMIKVLSLYLFVNFILMIIGTSFTFLEHTNFYNGMLTY", "aa_hexdigest": "9c09560b4704ffaf891fa0341b9952bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11693.949899999989, "isoelectric_point": 9.26889362335205 }, "id": "GPKLONFDEH_95", "locus": "GCA000006625_00055" }, { "type": "cds", "sequence": "contig_1", "start": 35490, "stop": 35699, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000169E242", "UniRef:UniRef90_UPI000169E242" ], "nt": "TTGAAAAATGATAATGAAGAATTAGAACTTTATCAACTAGGCCTTGGAAGATCAGTTAGAGTAATATGCTCAAAAATCGATAATATTTTATTTCCTTTACTTATTGATCATCATCATTTAGGATATGAAAGTCAATCTTATAATGATAAAGATACTAAAAAATATGATTATTGTCCTTTAAGAAAATATAATAAAAATCAAGATAACTAA", "aa": "MKNDNEELELYQLGLGRSVRVICSKIDNILFPLLIDHHHLGYESQSYNDKDTKKYDYCPLRKYNKNQDN", "aa_hexdigest": "22212f4e7b30e1513a8ae4164f8e7b9f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000169E242", "query_cov": 1.0, "subject_cov": 0.8846153846153846, "identity": 0.9420000000000001, "score": 141.0, "evalue": 1.22e-42, "valid": true, "uniref50_id": "UniRef50_UPI000169E242" }, "pscc": { "uniref50_id": "UniRef50_UPI000169E242", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000169E242" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8248.172999999997, "isoelectric_point": 6.249810981750489 }, "id": "GPKLONFDEH_96", "locus": "GCA000006625_00056" }, { "type": "cds", "sequence": "contig_1", "start": 35910, "stop": 36017, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAAGAAAATAAATAACAATAAAACTCCTCGCAATAAAGTTAAAAATAACAATGTTTCAAAAGATAATGATGACATTGTTATTTCTTTGCGTTATGAAAATTGA", "aa": "MSKKINNNKTPRNKVKNNNVSKDNDDIVISLRYEN", "aa_hexdigest": "142e6c0733274ab8e03bbab82b3baf06", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4104.563700000001, "isoelectric_point": 9.871673011779784 }, "id": "GPKLONFDEH_97", "locus": "GCA000006625_00057" }, { "type": "cds", "sequence": "contig_1", "start": 36190, "stop": 36303, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATAATGAATTCAAAGAACATAAATATAATAGTATTCCTAAAATAAATGATTTTAATGAACTAGAATATTTTAATTTAGATACTATTGATATATTGAATCAAGTTTGA", "aa": "MLYNEFKEHKYNSIPKINDFNELEYFNLDTIDILNQV", "aa_hexdigest": "91c27eee2901ca0c9b8db3996783b6f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4566.059700000002, "isoelectric_point": 4.433748435974121 }, "id": "GPKLONFDEH_98", "locus": "GCA000006625_00058" }, { "type": "cds", "sequence": "contig_1", "start": 36548, "stop": 36718, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGAATCGAAAAAATATCATTTAGAAATTACAACATCAAAAACCAATAAAACACATAAGTTTTCCAAGTTTGCACTTTTATTTTTAAAAGAAAAAATAATCGAATTAGATGAAAATAAAAATGAACTAAACAATATAATATCAAAATTTAATAATAGAAAAAAGTAA", "aa": "MKESKKYHLEITTSKTNKTHKFSKFALLFLKEKIIELDENKNELNNIISKFNNRKK", "aa_hexdigest": "49fe8d3f37cb08c3a29b58b47d26e2a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6754.809799999999, "isoelectric_point": 9.874316215515137 }, "id": "GPKLONFDEH_99", "locus": "GCA000006625_00059" }, { "type": "cds", "sequence": "contig_1", "start": 36728, "stop": 37201, "strand": "-", "frame": 3, "gene": null, "product": "putative ATP-binding protein UU034", "db_xrefs": [ "GO:0005524", "SO:0001217", "UniRef:UniRef50_Q9PRB1", "UniRef:UniRef90_Q9PRB1" ], "nt": "GTGATAGCACAAAAGAATAATAGTTCAGAAAAAATTGTTAATGGTGATATATTTTATCGTTATAGAGCGAGAACTGAAAAAATAAAATATGCAGAAATGGAACAAATTATTAATTGTAGAATGAAGCAAGAACGAGATAGCTTATTTAAGGTTTTTGAATTTATTAGAAATAATGGTGCTACTAATTTAGGAATAGTTGATTATAATAACGGAAAATTGTCCACTCCTTATGGAGTTGATGTTGTTTTAGAAAAAAATCTTATAGCAAAATTACTAAAAAAAGCAAAATTTATAAAAGAAGGTAGTTTTAGTGAAAATGAAGGAATACCAGTAATCAAAGTTACTGGAAATATAAATTTGGCAGAAGAAGTTCCAGTTCCATTAGAAAATTTAGATGAAACTTATCCCTATATTCAAAAACAATTAGCCGAAAAACTTAATATAACACCTCAAAAATTATATGCACTTATTTGA", "aa": "MIAQKNNSSEKIVNGDIFYRYRARTEKIKYAEMEQIINCRMKQERDSLFKVFEFIRNNGATNLGIVDYNNGKLSTPYGVDVVLEKNLIAKLLKKAKFIKEGSFSENEGIPVIKVTGNINLAEEVPVPLENLDETYPYIQKQLAEKLNITPQKLYALI", "aa_hexdigest": "73ecb8ccae3198a5bfc10491dd00956e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 36548, "stop": 37501, "observations": { "mutation": [ 36730, 37234, 37315 ], "stop": [ 36730, 37234, 37315 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRB1", "query_cov": 1.0, "subject_cov": 0.4422535211267606, "identity": 0.9940000000000001, "score": 305.0, "evalue": 7.09e-102, "gene_start": 1, "gene_end": 157, "reference_start": 139, "reference_end": 295, "reference_sequence": "MLISKHEERVKSILSARMENGNLRSRENTQVEFKQSFNKGNIAIYAKTMAAFSNNSGGYIIFGIKDSPRSIIGLQNSNFENIQQEDLTDSINNLFSPSINWELGSFILKEKEINSNGDVIIVQKIIGWIYTEESNIKPVIAQKNNSSEKIVNGDIFYRYRARTEKIKYAEMEQIINCRMKQERDSLFKVFEFIRNNGATNLGIVDYNNGKLSTPYGVDVVLEKNLIAKLLKKAKFIKEGSFSENEGIPVIKVTGNINLAEEVPVPLENLDETYPYIQKQLAEKLNITPQKLYALIWYFKMKESKKYHLEITTSKTNKTHKFSKFALLFLKEKIIELDENKNELNNIISKFNNRKK" }, "paralog": false, "identity": 0.9905362776025236, "score": 611.0, "evalue": 3.08e-225, "description": "Internal stop codon at 36730, 37234, 37315. Nonsense mutation around 36730, 37234, 37315.", "psc": { "uniref90_id": "UniRef90_Q9PRB1", "product": "Uncharacterized ATP-binding protein UU034", "uniref50_id": "UniRef50_Q9PRB1", "go_ids": [ "GO:0005524" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRB1", "UniRef:UniRef50_Q9PRB1", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRB1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRB1" ], "product": "Uncharacterized ATP-binding protein UU034" } }, "id": "GPKLONFDEH_100", "locus": "GCA000006625_00060" }, { "type": "cds", "sequence": "contig_1", "start": 37313, "stop": 37615, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04326.19" ], "nt": "ATGCTTATATCAAAACATGAAGAAAGAGTAAAGAGTATATTATCTGCTCGTATGGAAAATGGAAATTTGCGTAGTAGGGAAAATACTCAAGTTGAATTTAAACAATCATTTAATAAAGGCAACATTGCAATATATGCTAAAACTATGGCAGCATTTTCTAATAACAGTGGTGGATATATTATTTTTGGTATTAAAGATTCACCTCGTTCCATAATTGGGTTACAAAATAGTAATTTTGAAAATATTCAACAAGAAGATTTGACAGATTCTATTAATAATCTATTTTCCCCTTCTATAAATTGA", "aa": "MLISKHEERVKSILSARMENGNLRSRENTQVEFKQSFNKGNIAIYAKTMAAFSNNSGGYIIFGIKDSPRSIIGLQNSNFENIQQEDLTDSINNLFSPSIN", "aa_hexdigest": "144708f0e3b3abb5098f652cef13abf5", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04326.19", "name": "SLFN_AlbA_2", "length": 75, "aa_cov": 0.73, "hmm_cov": 0.9466666666666667, "evalue": 3.458935401275379e-17, "score": 58.665435791015625, "start": 27, "stop": 99 } ], "seq_stats": { "molecular_weight": 11243.477199999988, "isoelectric_point": 8.057597160339355 }, "id": "GPKLONFDEH_101", "locus": "GCA000006625_00061" }, { "type": "cds", "sequence": "contig_1", "start": 37719, "stop": 38459, "strand": "-", "frame": 2, "gene": null, "product": "putative protein UU035", "db_xrefs": [ "RefSeq:WP_012316989.1", "SO:0001217", "UniParc:UPI000165F8B9", "UniRef:UniRef100_Q9PRB0", "UniRef:UniRef50_Q9PRB0", "UniRef:UniRef90_Q9PRB0" ], "nt": "ATGTTGAGTAACAAAAAATTTTTAGATTTAGATATTATAAAGGAATCTTTTGATAATTATTTGAGTGTTGGAACGTCTAGAAGTACTAAAAAATTAAAAACATTACATGGGAGTATAGCAAAAGATTTAGAAGAATTGTTAGGCAAAGAATACAATATTAAATCCCAAGGTTATAGAGATGATAAAGAAGAATGTATTGATGGTAGATATTACAACAAAAAAGTTGATATAACAATTAAAAATAATGGAAAATCAATTGCTGGACTAGCAATTAAATTTGTTATGAGTAATTATTCTCAAAATAGTAATAATTATTTTGAGAATATGTTAGGCGAAACAGCTAATTTACGATCTAATTCAGTTCCTTATTTTCAAATTTTTATAATTTTTGATAAAGTTCCATACTATAAAAGTGATGGTGTATTACAAAAATATGATAATATTTCAAACCACAATTTGGAAAAATATTTAAAATTATCAGATGATGACCCTAAAGTTTATTTTCATACACCAGATAAAACGCTCTTCATTTTACTTAGTTTGAAAGAAATTAGTGAGGAAATTAAAAATAAAAAAGATTACATAAGATGTTATAAAGAAATTATGAATGATGAAGACTTACTAAGTTATTCAAACAAAATCGATGATTTATTTGGTAATTCTATAATATTAAATAATTATGAAGATTTTATCAAAAGAATGTGCGCTCTTATTATAGGCTATCAAAAAATAAAAACATAA", "aa": "MLSNKKFLDLDIIKESFDNYLSVGTSRSTKKLKTLHGSIAKDLEELLGKEYNIKSQGYRDDKEECIDGRYYNKKVDITIKNNGKSIAGLAIKFVMSNYSQNSNNYFENMLGETANLRSNSVPYFQIFIIFDKVPYYKSDGVLQKYDNISNHNLEKYLKLSDDDPKVYFHTPDKTLFILLSLKEISEEIKNKKDYIRCYKEIMNDEDLLSYSNKIDDLFGNSIILNNYEDFIKRMCALIIGYQKIKT", "aa_hexdigest": "3aaf1c4dc84f86d5fc5a09ff12fcb825", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI000165F8B9", "ncbi_nrp_id": "WP_012316989.1", "uniref100_id": "UniRef100_Q9PRB0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8B9", "RefSeq:WP_012316989.1", "UniRef:UniRef100_Q9PRB0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRB0", "uniref90_id": "UniRef90_Q9PRB0" }, "psc": { "uniref90_id": "UniRef90_Q9PRB0", "product": "Uncharacterized protein UU035", "uniref50_id": "UniRef50_Q9PRB0" }, "pscc": { "uniref50_id": "UniRef50_Q9PRB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRB0" ], "product": "Uncharacterized protein UU035" }, "genes": [], "id": "GPKLONFDEH_102", "locus": "GCA000006625_00062" }, { "type": "cds", "sequence": "contig_1", "start": 38712, "stop": 39545, "strand": "-", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B75", "UniRef:UniRef100_Q9PRA9", "UniRef:UniRef50_Q9PRA9", "UniRef:UniRef90_Q9PRA9" ], "nt": "ATGAAAATAAATATATATGATTCTAAAATGGATTTTGTTATTGGAAATCCTCCATATGTTAGAATTCATAATTTGATTAAAGAACCCAATTTAATTAAAAATTATTTATTTAGTAATAAAGGAATGACTGATTTGTATATTATATTTTATGAAATTGGTATAAAAATGTTAAATAAAAATGGAATTTTATGCTACATCACACCATCTTCTTTTTTTACAAGTTTAGCAGGATTAAATATGCGCTTGTTTTTGAGTGAATATAAATTAATCGAAAGTGTTTGTGATTTAAAACATTTTCAAGCTTTTAATGCTACTGTTTATACAACTATTATTTGTTTAAATAAGAGTAAAAAAAATGAATGTGTTGAATATTATGAATTTGATGAGAAAAAATTAAAACCTTGTTTTATTGAAAAAATATCCCACAACGATTATTTTATAAAAAATAATTATTATTTTGGACCTAAACAAAAACTTATTTTTTTAAAAAAAGTTTTACTTTCAAATAAAACCACAAACATATTAGTAAAGAATGGTTATGCAACATTGTCAGATAAAATATTTATTAATGATTTTGACTTTGAATCAAAATTCATTATTCCTGTCATTAAAGCTTCGAAAGGAGAACTAAAAAAAATATTTTATCCATACGATAAAGAAAATTTTTTAATTGATGAATTAGTGTTAAAAAAAGATTTAAAAATGTACAATTATTTAAAATTTAATAAAAATAATTTACTAAAAAAAATCATGATATTAGTTCAAAAAATTGTTGATATGCATTTGGTAGAAGTCAAGGAATTAAAGATACTTATAAGGATAAAATTGCAATAA", "aa": "MKINIYDSKMDFVIGNPPYVRIHNLIKEPNLIKNYLFSNKGMTDLYIIFYEIGIKMLNKNGILCYITPSSFFTSLAGLNMRLFLSEYKLIESVCDLKHFQAFNATVYTTIICLNKSKKNECVEYYEFDEKKLKPCFIEKISHNDYFIKNNYYFGPKQKLIFLKKVLLSNKTTNILVKNGYATLSDKIFINDFDFESKFIIPVIKASKGELKKIFYPYDKENFLIDELVLKKDLKMYNYLKFNKNNLLKKIMILVQKIVDMHLVEVKELKILIRIKLQ", "aa_hexdigest": "e7c70d896c7a75f50c7d5e3280feeb24", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1B75", "uniref100_id": "UniRef100_Q9PRA9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B75", "UniRef:UniRef100_Q9PRA9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRA9", "uniref90_id": "UniRef90_Q9PRA9" }, "psc": { "uniref90_id": "UniRef90_Q9PRA9", "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_Q9PRA9" }, "pscc": { "uniref50_id": "UniRef50_Q9PRA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRA9" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "GPKLONFDEH_103", "locus": "GCA000006625_00063" }, { "type": "cds", "sequence": "contig_1", "start": 39643, "stop": 39906, "strand": "-", "frame": 1, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRA8", "UniRef:UniRef90_Q9PRA8" ], "nt": "ATGAAAGAATTATTGAGATTGACTAAGGTTAAACGCTATGGAAAAGTTTATACACCTGATTATTTAGTAAATATAATACTTGATAAAGGGCATTATATTAATGGAAATATAAATAAAAAACATGTAATTGATAATAGTTGTGGAGATGGGCAATTTTTAACTTATATAGTTGATAGATATTGCAAAGATTATCTTATAAATAATAATGATTTAATTGAGTTAAAAAAAACAATTAGAAACATTTATACATGGTATAGAAATTGA", "aa": "MKELLRLTKVKRYGKVYTPDYLVNIILDKGHYINGNINKKHVIDNSCGDGQFLTYIVDRYCKDYLINNNDLIELKKTIRNIYTWYRN", "aa_hexdigest": "f62570cd68ea7c3f0eba7681b27a80db", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_Q9PRA8", "query_cov": 1.0, "subject_cov": 0.9886363636363636, "identity": 1.0, "score": 185.0, "evalue": 1.4e-59, "valid": true, "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PRA8" }, "pscc": { "uniref50_id": "UniRef50_Q9PRA8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRA8" ], "product": "Unique hypothetical" }, "genes": [], "id": "GPKLONFDEH_104", "locus": "GCA000006625_00064" }, { "type": "oriC", "sequence": "contig_1", "start": 40240, "stop": 40329, "strand": "?", "product": "origin of replication", "nt": "TTTATTAATCACAATAATTTAGTATTTATCCAAAATTTTAATAACAATATTAGTAGTATAATGACTTGTAAATAATACAAGGAGATTTTG", "id": "GPKLONFDEH_1302" }, { "type": "cds", "sequence": "contig_1", "start": 41140, "stop": 41298, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00030D0A09" ], "nt": "GTGCAAGAAGTAATAGTTAATGCTTTGATTCATCGAGATTATTCAATTATTGGTAGTGAAGTACATATTGACATGTATGATGATAGATTAGAAGTATATTCTCCTGGTGGTATGTTTGATGGGAATTTTGTCCAAAATATCGACCTTTATAATGTATGA", "aa": "MQEVIVNALIHRDYSIIGSEVHIDMYDDRLEVYSPGGMFDGNFVQNIDLYNV", "aa_hexdigest": "a61c61bc305661c148d053acefd74138", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00030D0A09", "query_cov": 1.0, "subject_cov": 0.8125, "identity": 0.519, "score": 64.7, "evalue": 9.44e-13, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_UPI00030D0A09" }, "pscc": { "uniref50_id": "UniRef50_UPI00030D0A09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00030D0A09" ], "product": "ATP-binding protein" }, "genes": [], "id": "GPKLONFDEH_105", "locus": "GCA000006625_00065" }, { "type": "cds", "sequence": "contig_1", "start": 41341, "stop": 41700, "strand": "+", "frame": 1, "gene": null, "product": "HTH domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A133KCP2" ], "nt": "ATGAATTTAATGGAAAGAAGAGGTTCTGGTTTAAAAAATATTTTAGAAATTTATGCATCTAAAGAAAATTATCGAGAAGATTTAAAACCGGAGTTTCGTTCAACTGAATCTGCTTTCTTTGTTGTTTTAAAAAATTTAAATTATCAAGAGCAAAATATATCAAAAAATGAAAATCAAAAAATATCCCCTAGCCAAAGACGTAAAAAAATCATAGAATTTATCGGACAAAATCCAAAAACAACAGCTTATGAATTAACAAAAATATTTTTTGTTTCAATTAATACCATTGAACGCGATCTAGCTAAATTAGTACATGATGGATATGTTGAATTTGTGGGATCATCAAAAAATGGTGAATGA", "aa": "MNLMERRGSGLKNILEIYASKENYREDLKPEFRSTESAFFVVLKNLNYQEQNISKNENQKISPSQRRKKIIEFIGQNPKTTAYELTKIFFVSINTIERDLAKLVHDGYVEFVGSSKNGE", "aa_hexdigest": "490c031b42e26cdcb1f88b5b28877d08", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003398ADF9", "query_cov": 1.0252100840336134, "subject_cov": 0.8299319727891157, "identity": 0.5329999999999999, "score": 124.0, "evalue": 3.6e-34, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A133KCP2" }, "pscc": { "uniref50_id": "UniRef50_A0A133KCP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A133KCP2" ], "product": "HTH domain protein" }, "genes": [], "id": "GPKLONFDEH_106", "locus": "GCA000006625_00066" }, { "type": "cds", "sequence": "contig_1", "start": 41797, "stop": 42066, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07992.20" ], "nt": "GTGAAAAAATATGATGTAATTGTCATTGGTGCTGGACACGCAGGATTAGAAGCAGCTTTTGCAACTTCTAATCTTAATTTACAAACAGCATTAATTACATTGGATGAAAAAGGGATTGGGATGATGCCATGCAATCCTTCAATAGGAGGGCCGGCTAAAGGAATTGTTACACGTGAAATTGATGCCCTTGGTGGTATTCAAGGTAAGGCAGCTGATGCTACAACAATGCAAATGAAAATCTTAAATTCATCAAAAGGACCAGGTGTTTGA", "aa": "MKKYDVIVIGAGHAGLEAAFATSNLNLQTALITLDEKGIGMMPCNPSIGGPAKGIVTREIDALGGIQGKAADATTMQMKILNSSKGPGV", "aa_hexdigest": "d6186cc091bff2597a6971512abb283a", "start_type": "GTG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI002852D7CD", "query_cov": 0.9662921348314607, "subject_cov": 0.8269230769230769, "identity": 0.593, "score": 108.0, "evalue": 3.82e-29, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A967GLJ3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 50, "aa_cov": 0.5617977528089888, "hmm_cov": 0.98, "evalue": 1.0214110005484274e-07, "score": 27.2376708984375, "start": 4, "stop": 53 } ], "seq_stats": { "molecular_weight": 9040.468699999994, "isoelectric_point": 7.879728889465332 }, "id": "GPKLONFDEH_107", "locus": "GCA000006625_00067" }, { "type": "cds", "sequence": "contig_1", "start": 42349, "stop": 43227, "strand": "+", "frame": 1, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "COG:COG0445", "COG:J", "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660", "SO:0001217", "UniRef:UniRef50_Q6MRU5", "UniRef:UniRef90_Q9PRA6" ], "nt": "GTGAAATTAGGATTTGAGTTAATTCGTTTAAAAACAGGGACTCCTGCAAGAATTAAAAAAGATTCGATTGATTTTACAAATATGATTCTTGAACCTGGTACAAATCAAAAAATTGCTTTTAGTCACTATCACCCTGTTTATAAACCTTATGACGAACAACTTCCTTGTCATATTATTTATACAAACGAACAAACTCACCAAATTATTCGAGAAAATTTAAATAAATCAGCAATGTATGGGGGTATGATTTCTGGAATTGGGCCACGTTATTGCCCTTCAATTGAAGATAAAATTGTTAAATTTAGTGAAAAACCGCGTCACCAAATTTTTGTTGAACCAGAATCTTATGAATTAGATTCAATGTACTTAGGTGGTTTTTCTACTTCAATGCCAATCGATGTTCAAGAAAAAATGATTCGTAGTTTACCAGGATTAGAAAACTGTGAAATACTAAAATATGCTTACGCTATTGAATATGATGCTATTGATCCAACCCAACTATATCCTAGTTTAGAATCAAAATTAGTTAATAATTTATTTTTTGCTGGTCAAATCAATGGAACTAGTGGCTATGAAGAAGCAGCGGCCCAAGGATTAATGGCTGCAATTAATGTTAATCAAAAATATCAAAATAAAGAACCTGTCATTTTAGGTCGTGATCAAGCATATATTGGCGTTATGATTGATGATATTGTTACCAAAGGCGTTGTTGAACCATATCGTTTACTAACAAGCCGTGCGGAACACCGATTAGCATTAAGAAATGATAATGCTGATGATCGATTAATGAAAATTGGTTTTGAAATTGGTTTATTAAAACCAGAAGTATATGACCAATACTTAAATAATTTAAAACAAATTAAAGAAATCTTAAACTGA", "aa": "MKLGFELIRLKTGTPARIKKDSIDFTNMILEPGTNQKIAFSHYHPVYKPYDEQLPCHIIYTNEQTHQIIRENLNKSAMYGGMISGIGPRYCPSIEDKIVKFSEKPRHQIFVEPESYELDSMYLGGFSTSMPIDVQEKMIRSLPGLENCEILKYAYAIEYDAIDPTQLYPSLESKLVNNLFFAGQINGTSGYEEAAAQGLMAAINVNQKYQNKEPVILGRDQAYIGVMIDDIVTKGVVEPYRLLTSRAEHRLALRNDNADDRLMKIGFEIGLLKPEVYDQYLNNLKQIKEILN", "aa_hexdigest": "12d061904c223385d76d4e042702b159", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003008428B", "query_cov": 0.863013698630137, "subject_cov": 0.972972972972973, "identity": 0.603, "score": 311.0, "evalue": 1.48e-103, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A535SKJ6", "kegg_orthology_id": "K03495" }, "genes": [ "mnmG" ], "truncated": "both", "pseudogene": { "start": 42049, "stop": 43530, "observations": { "mutation": [ 42064, 42106, 43225 ], "stop": [ 42064, 42106, 43225 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRA6", "query_cov": 1.0, "subject_cov": 0.4755700325732899, "identity": 0.997, "score": 587.0, "evalue": 6.92e-207, "gene_start": 1, "gene_end": 292, "reference_start": 185, "reference_end": 476, "reference_sequence": "MKKYDVIVIGAGHAGLEAAFATSNLNLQTALITLDEKGIGMMPCNPSIGGPAKGIVTREIDALGGIQGKAADATTMQMKILNSSKGPGVWAIRAQIDKIAYQRWFKQQIKQQKNLDLIIAEVSDLLVENNIVKGVVLSDQKIIQADYVIITTGTYLKSITHRGSVCVDEGADGTKNAKFLSDALVKLGFELIRLKTGTPARIKKDSIDFTNMILEPGTNQKIAFSHYHPVYKPYDEQLPCHIIYTNEQTHQIIRENLNKSAMYGGMISGIGPRYCPSIEDKIVKFSEKPRHQIFVEPESYELDSMYLGGFSTSMPIDVQEKMIRSLPGLENCEILKYAYAIEYDAIDPTQLYPSLESKLVNNLFFAGQINGTSGYEEAAAQGLMAAINVNQKYQNKEPVILGRDQAYIGVMIDDIVTKGVVEPYRLLTSRAEHRLALRNDNADDRLMKIGFEIGLLKPEVYDQYLNNLKQIKEILNWLKTTTVGQIDDLKFTTLKTNSYLIDYLKRPEIKLNDLLIYCPIKIEDEQIINKVQIQVKFEGYIKNQEENLKQLKRLNNIKLHAIVDYKEVPNISLETIDKLNKIKPLDLEQASRISGVNLTDIAMIKYYLERIKND" }, "paralog": false, "identity": 0.9939148073022313, "score": 966.0, "evalue": 0.0, "description": "Internal stop codon at 42064, 42106, 43225. Nonsense mutation around 42064, 42106, 43225.", "psc": { "uniref90_id": "UniRef90_Q9PRA6", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_Q6MRU5", "cog_id": "COG0445", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRA6", "UniRef:UniRef50_Q6MRU5", "COG:COG0445", "COG:J", "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MRU5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MRU5" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" } }, "id": "GPKLONFDEH_108", "locus": "GCA000006625_00068" }, { "type": "cds", "sequence": "contig_1", "start": 43498, "stop": 43641, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13932.14" ], "nt": "GTGCCAAATATTTCTTTAGAAACAATTGATAAATTAAATAAGATTAAACCTCTTGATCTTGAACAAGCAAGTCGGATTAGTGGGGTTAATTTAACAGACATTGCAATGATTAAATATTATTTAGAAAGAATTAAAAATGACTAG", "aa": "MPNISLETIDKLNKIKPLDLEQASRISGVNLTDIAMIKYYLERIKND", "aa_hexdigest": "cee43cc5cd39e4c473ff2c733c1d7396", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13932.14", "name": "SAM_GIDA_C", "length": 37, "aa_cov": 0.7872340425531915, "hmm_cov": 1.0, "evalue": 1.4697437127005196e-13, "score": 46.02214431762695, "start": 1, "stop": 37 } ], "seq_stats": { "molecular_weight": 5419.2748, "isoelectric_point": 6.130392265319824 }, "id": "GPKLONFDEH_109", "locus": "GCA000006625_00069" }, { "type": "cds", "sequence": "contig_1", "start": 43700, "stop": 44014, "strand": "+", "frame": 2, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "COG:COG0357", "COG:J", "EC:2.1.1.-", "EC:2.1.1.170", "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043", "KEGG:K03501", "SO:0001217", "UniRef:UniRef50_Q9PRA5", "UniRef:UniRef90_Q9PRA5" ], "nt": "ATGAGTTTTGAAAAGTATAAAACAATTATTCAAAAATATAATCAAATTTTTAATTTGACAAGACTTGATAGTGATGATAAGATTTACCAAAATTTCTTTTTAGATTCACTTGCACCTTATAAAGAACTAGATTTTTTTACACAAAATACTAATCTTAAATTAATTGATATTGGTTCTGGTTCTGGAATTCCAGGGGTTGTTTTAAAGATTATTTTTAAAAATTTAAATTTAACATTATTAGAAGCTAATCAAAAACGATGTGAATTTCTTAAAATATTAACTCAAGAATTAGGTTTAAATGATGTCTTAATTTGA", "aa": "MSFEKYKTIIQKYNQIFNLTRLDSDDKIYQNFFLDSLAPYKELDFFTQNTNLKLIDIGSGSGIPGVVLKIIFKNLNLTLLEANQKRCEFLKILTQELGLNDVLI", "aa_hexdigest": "839d73caf27fd133412aca0503e52931", "start_type": "ATG", "rbs_motif": null, "genes": [ "rsmG" ], "truncated": "both", "pseudogene": { "start": 43634, "stop": 44317, "observations": { "mutation": [ 43679, 44012, 44240 ], "stop": [ 43679, 44012, 44240 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PRA5", "query_cov": 1.0, "subject_cov": 0.4406779661016949, "identity": 1.0, "score": 204.0, "evalue": 5.46e-65, "gene_start": 1, "gene_end": 104, "reference_start": 23, "reference_end": 126, "reference_sequence": "MTRKDFFNVLTRYFPWVDEQTFMSFEKYKTIIQKYNQIFNLTRLDSDDKIYQNFFLDSLAPYKELDFFTQNTNLKLIDIGSGSGIPGVVLKIIFKNLNLTLLEANQKRCEFLKILTQELGLNDVLIWNMRAEDLTQSMRESFDIATSRAVASLDKILEISAAFVKVNGYLIQPKSIKFYEEELKAKNIIKTLYLERIALKDFWENDYHHLVGVYLKKQITPLQFPRPWNLILKKPL" }, "paralog": false, "identity": 0.986784140969163, "score": 437.0, "evalue": 3.43e-159, "description": "Internal stop codon at 43679, 44012, 44240. Nonsense mutation around 43679, 44012, 44240.", "psc": { "uniref90_id": "UniRef90_Q9PRA5", "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.-", "2.1.1.170" ], "uniref50_id": "UniRef50_Q9PRA5", "cog_id": "COG0357", "cog_category": "J", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PRA5", "EC:2.1.1.-", "EC:2.1.1.170", "UniRef:UniRef50_Q9PRA5", "COG:COG0357", "COG:J", "KEGG:K03501", "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRA5" ], "product": "Ribosomal RNA small subunit methyltransferase G" } }, "id": "GPKLONFDEH_110", "locus": "GCA000006625_00070" }, { "type": "cds", "sequence": "contig_1", "start": 44018, "stop": 44242, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGCTGAAGATTTAACACAATCGATGCGTGAATCTTTTGATATTGCAACTTCAAGAGCTGTTGCATCATTAGATAAAATTTTAGAAATTTCAGCCGCATTTGTTAAAGTTAATGGTTATTTAATTCAACCAAAATCAATAAAATTTTATGAAGAAGAACTAAAAGCAAAAAATATTATAAAAACTTTGTATTTAGAAAGAATCGCTTTAAAAGATTTTTGA", "aa": "MRAEDLTQSMRESFDIATSRAVASLDKILEISAAFVKVNGYLIQPKSIKFYEEELKAKNIIKTLYLERIALKDF", "aa_hexdigest": "926926e3cf635271e63ff03845b418ec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8513.834299999995, "isoelectric_point": 7.963924598693847 }, "id": "GPKLONFDEH_111", "locus": "GCA000006625_00071" }, { "type": "cds", "sequence": "contig_1", "start": 44367, "stop": 44729, "strand": "+", "frame": 3, "gene": "sWEET", "product": "putative protein UU041", "db_xrefs": [ "COG:COG4095", "COG:G", "GO:0005886", "RefSeq:WP_004025823.1", "SO:0001217", "UniParc:UPI0000139172", "UniRef:UniRef100_Q9PRA4", "UniRef:UniRef50_Q9PRA4", "UniRef:UniRef90_Q9PRA4" ], "nt": "ATGAGTTCATTACCAGTTATTATTTTAGTAATTGGCATTTTTGGGTCTATTTTTCTAGTAATAGGATATATTCCCCAAGTAATTAAAGTTATCAAAACAAAACGAACAGATGGAATTTCATTAACATTTTTAATTTCTTTAAACATCGCTTGTTTTCTATTTGTAATATATAGTATATTAGTAATGATTTTTAACAAACATAATGGTATTCCTACTGCTTTACCATTATGTCTTGCAAACACCATAGTAGGAATTTTAGGTCTAGTTATTTTAATTTATAAAGTTAAAAACATTAAAAAAGCTAAATTATATTTAATGGATGAAAAGACATATTATGAAAAATATGTCTTAAATAATTTATAA", "aa": "MSSLPVIILVIGIFGSIFLVIGYIPQVIKVIKTKRTDGISLTFLISLNIACFLFVIYSILVMIFNKHNGIPTALPLCLANTIVGILGLVILIYKVKNIKKAKLYLMDEKTYYEKYVLNNL", "aa_hexdigest": "e1096985c2414dd79f62d657345aad65", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000139172", "ncbi_nrp_id": "WP_004025823.1", "uniref100_id": "UniRef100_Q9PRA4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139172", "RefSeq:WP_004025823.1", "UniRef:UniRef100_Q9PRA4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRA4", "uniref90_id": "UniRef90_Q9PRA4" }, "psc": { "uniref90_id": "UniRef90_Q9PRA4", "gene": "SWEET", "product": "Uncharacterized protein UU041", "uniref50_id": "UniRef50_Q9PRA4", "cog_id": "COG4095", "cog_category": "G", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRA4" ], "product": "Uncharacterized protein UU041" }, "genes": [ "sWEET" ], "id": "GPKLONFDEH_112", "locus": "GCA000006625_00072" }, { "type": "cds", "sequence": "contig_1", "start": 44729, "stop": 45145, "strand": "+", "frame": 2, "gene": "sWEET", "product": "putative protein UU042", "db_xrefs": [ "COG:COG4095", "COG:G", "GO:0005886", "RefSeq:WP_010891664.1", "SO:0001217", "UniParc:UPI0000139177", "UniRef:UniRef100_Q9PRA3", "UniRef:UniRef50_Q9PRA3", "UniRef:UniRef90_Q9PRA3" ], "nt": "ATGTCTCCAATTAGTATTGAATTAATAATTCAAATAAGTATTGGATTAAGTGCTAGCCTAATTTTATTGTTTGCTTTCTTACCACAAACACTTTTAACTATTAAAACTAAAAATACTGCTGCATTAACAATTAGTATGTTTATTATTTGTTTTATTGCACGTTTATGTTTTAGTTTAAGTGCTATTCTAACAATTATCGTCTATATTCATAATCAGAATTATGGTTTATCTTTATATGCGCTTACACTTCCTGTTTTAATATGTCATGGTATTAATATGTTATTAAATTTAATTATTGCTTTTATAAAAATCAATAATGTTTATAAAGCCAAAATCCATAAAATGAATGAGAATGAATATATTATTTTTGCTTATGCTCAAAAATTAAAGGAAAAAGTATCGATAAAAAATAAATAA", "aa": "MSPISIELIIQISIGLSASLILLFAFLPQTLLTIKTKNTAALTISMFIICFIARLCFSLSAILTIIVYIHNQNYGLSLYALTLPVLICHGINMLLNLIIAFIKINNVYKAKIHKMNENEYIIFAYAQKLKEKVSIKNK", "aa_hexdigest": "b51235f50006ffb26fdec0018edb68a6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139177", "ncbi_nrp_id": "WP_010891664.1", "uniref100_id": "UniRef100_Q9PRA3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139177", "RefSeq:WP_010891664.1", "UniRef:UniRef100_Q9PRA3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PRA3", "uniref90_id": "UniRef90_Q9PRA3" }, "psc": { "uniref90_id": "UniRef90_Q9PRA3", "gene": "SWEET", "product": "Uncharacterized protein UU042", "uniref50_id": "UniRef50_Q9PRA3", "cog_id": "COG4095", "cog_category": "G", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PRA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PRA3" ], "product": "Uncharacterized protein UU042" }, "genes": [ "sWEET" ], "id": "GPKLONFDEH_113", "locus": "GCA000006625_00073" }, { "type": "cds", "sequence": "contig_1", "start": 45312, "stop": 46196, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04200.17" ], "nt": "ATGAAAAAGTTAAAATTAATCACTTTAAGTTTATCGCCTATTCCTATCGTCGCGATCATGGCTACAGCATGTAGTACAACAAAAAAAGAAAAAGAAGTAGATCATAAACAAGATGAATTAACTACTATAAATAATAATTTACAATTAGAATATCCACAAAACAAAGATATAGAAACATCAGCAGCAATAAAGGAAGAAATAAAGCCTTTAAATTTGCCGTCTGGTGTTGTTTTTAGCATTAAAGAAATTAGTCCTAAAGCAAATGATGAAAGTACATTAATAGTTAAATACACCCTTAAAAAGGGAAACATAACTAAAGAATTTAAAAAAGAAATTAATGGATTTAAAAAAATTCGTAAAGATCAAAATATCGCTAATCTAAAAGACATGCATGATGATTTTAAAACAATTTTTAATAACATTAAATTAAAAGATACGTTCGATTTTAAACTAAAAGGTCTTTTTAACTCAAATTTTGGTGAATACGATAAATTATTACCATCACAAATTATTAACAATTTTGCACAAGGTGTAGAAACTAGAATTAATACTAACCAAGATCGTTTAGGTATTGAAGTTATAGATGTTAGTTATCCAAATAAAAATTTTGGTTCTGCTAATCGTGAAGGTAATTTAAAAATGTCACTATTAGTAACTGATAAAAAAACAAAACAAGTTTTCATTAAATCAATAATTGCGTATGGTTTTAAAACTAATGCAATGGGTTTAGATGAGAATGGCTCAATTCCAGGTGGTGGTGCTGATCTTGTTAAACCTAAAGTTGATGAAAATAATTATTTTAATAAAACTCAATTAGAAAGATATGAAATTGATAATCAAGCTTATTTAAATGGTTTAAAAGGCCAACATATGGGTAAAACTTGA", "aa": "MKKLKLITLSLSPIPIVAIMATACSTTKKEKEVDHKQDELTTINNNLQLEYPQNKDIETSAAIKEEIKPLNLPSGVVFSIKEISPKANDESTLIVKYTLKKGNITKEFKKEINGFKKIRKDQNIANLKDMHDDFKTIFNNIKLKDTFDFKLKGLFNSNFGEYDKLLPSQIINNFAQGVETRINTNQDRLGIEVIDVSYPNKNFGSANREGNLKMSLLVTDKKTKQVFIKSIIAYGFKTNAMGLDENGSIPGGGADLVKPKVDENNYFNKTQLERYEIDNQAYLNGLKGQHMGKT", "aa_hexdigest": "065fbe84e940ba8c3b66727fe083a07c", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 84, "aa_cov": 0.2653061224489796, "hmm_cov": 0.9880952380952381, "evalue": 1.361290193512888e-09, "score": 33.82155227661133, "start": 40, "stop": 117 } ], "seq_stats": { "molecular_weight": 33172.67369999999, "isoelectric_point": 9.186696434020995 }, "id": "GPKLONFDEH_114", "locus": "GCA000006625_00074" }, { "type": "cds", "sequence": "contig_1", "start": 46203, "stop": 46388, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTCCTGAACTTGGTGATAATGAACAAAAAATTAATGAATTTGATCAAAAAGCTAAAGGTGTAAGTCAAGATGCTTATGCGTCTGCTGCTTATAAAGGTTTTACACTCCCAGTGTATGATAAAAATGGTGATTTTAAAGGTTTATCAATTAATGAAAGTAGTTACGGCCAAGCTCGTTCATGA", "aa": "MRPELGDNEQKINEFDQKAKGVSQDAYASAAYKGFTLPVYDKNGDFKGLSINESSYGQARS", "aa_hexdigest": "ecf1609f4477adc1014328bb6632acc9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6736.274200000001, "isoelectric_point": 5.170722770690916 }, "id": "GPKLONFDEH_115", "locus": "GCA000006625_00075" }, { "type": "cds", "sequence": "contig_1", "start": 46662, "stop": 46934, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGAAATTAATGGATAGTGTTAAAACTAAAACAACTTTACGTTTAAGTAATTTAGATGAAAATATTTATCGTTTTGGTTTTGCAAGTAAAAATAACAAATTTTTATTAAATCATGGTCTTAAAAAAATATATGATGGTCGAAATTTCTTAAAAACTAAACCTACAGAATACTTAACAGAACAACAAAAAGAAAAATACAAAGATGCAGGTAGTTTTGTTGATTTTGCAGTTTTTGAATTAGATTTCGAACAATTAAAATTACTTAATGTTTGA", "aa": "MKLMDSVKTKTTLRLSNLDENIYRFGFASKNNKFLLNHGLKKIYDGRNFLKTKPTEYLTEQQKEKYKDAGSFVDFAVFELDFEQLKLLNV", "aa_hexdigest": "3b9267d984ca6b0677ab6989f4a9f13c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 87, "aa_cov": 0.9444444444444444, "hmm_cov": 0.9310344827586207, "evalue": 2.5439386948130678e-17, "score": 59.08718490600586, "start": 2, "stop": 86 } ], "seq_stats": { "molecular_weight": 10618.115699999995, "isoelectric_point": 9.330783271789553 }, "id": "GPKLONFDEH_116", "locus": "GCA000006625_00076" }, { "type": "cds", "sequence": "contig_1", "start": 47337, "stop": 47828, "strand": "+", "frame": 3, "gene": null, "product": "MIP family Ig-specific serine endopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "nt": "TTGTCTTATGCGATTGGTTACCGTAGTTTTATCGATAAACCTGGTGTTCTAGATGCCTTTATTTCTGCTCCTCATACTGGCAATGATTTATATAAATCTCTTGATGGTAAAAAATATATTAATATGGGTTTGGAATATATGCCTAGACATTATGCTCCAGCAGGAGGTGCATCTGGTTCAAGTGTGCGAAATCAAAACAATGAAATTGTAGGAATTTATCATGTAAGTAATGAATTTGCTAGCACCGGCTTAGCAACTGCTTTTCGTTCTGAAGGATATGATTATAAAGGCTTATATGGTGATAAATACAAAGAATTACCACAATATGACTTAATTTATGGCGGTGGAAAAGATCAAGCTACAGGTCAATCATATCGTGAAAAAATGAAAGAAATATATAAAAATAATAACAATATTAAAACAGCATTATTTCCAAATGGTTTTGACCAATATGATGAAAAGTTTAAATTTAATAACAATCAAAGTAAATAA", "aa": "MSYAIGYRSFIDKPGVLDAFISAPHTGNDLYKSLDGKKYINMGLEYMPRHYAPAGGASGSSVRNQNNEIVGIYHVSNEFASTGLATAFRSEGYDYKGLYGDKYKELPQYDLIYGGGKDQATGQSYREKMKEIYKNNNNIKTALFPNGFDQYDEKFKFNNNQSK", "aa_hexdigest": "9207ca2bff0c569870d3fd2b3af00d0a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AFE92B4", "query_cov": 0.9570552147239264, "subject_cov": 0.9629629629629629, "identity": 0.603, "score": 179.0, "evalue": 5.89e-55, "valid": false, "product": "MIP family Ig-specific serine endopeptidase", "uniref50_id": "UniRef50_UPI0021DFAD80" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFAD80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "product": "MIP family Ig-specific serine endopeptidase" }, "genes": [], "id": "GPKLONFDEH_117", "locus": "GCA000006625_00077" }, { "type": "cds", "sequence": "contig_1", "start": 47851, "stop": 48576, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTATTAAACGTAAAACAAAATTATTAACGATTACAATTGGTGCAGTTGCTGTTAGTTCAATCTTATTAGGAGGAATTTTTTATGGTACAAGTCAAAAAAGTCCCTCAAGTTTTGGTATTGCTTCTATTGATCAAAAAGAAAATTTTATTAATAAAGACAATTTAGATTACCAAAAAGCAAGACCATCAATTAAAGATAGTAACTTAAAAGAAATTCCCAAACCTAAACCTCAACCAAAACCTAAGCCTCAACCAACTCCATTTCCAGATCCCATCCCAACACCTCCAAAAAAAGAAGAGCTAAAAAAACCAGATATTAAACCAGAAGAACCTAAAAAACCTGAAATTAAACCAGAACCAAAACCTGAGCCTATTCCTCAACCAGCCCCTCCTATTGAAACAAAACCAAAAGAAGAATTATTACCACCCAACCCTCCGCCTCCAAAAGAAGAACCAAAACCAGAACCAAATCCGCAACCACAGCCACAACAGGTTCCAAACAATAGTAATTCACGAATTATAGAAATTAATGGTGTAAGAGTGGAAGCTGAAGTTGAAGTCCCTCCCCCTCGTGATATTGCTGAATATGATAAACAAAATAATCTGGTTAACCCTAATCCATACATTAATGATAGTGTTGGTAAAATTAAAAACGTAAAGGTTACCGATGAATTGCGAAAAGCAACAGGTAAATTGGTCCAAGGAAATTTAGGACGTTGA", "aa": "MKFIKRKTKLLTITIGAVAVSSILLGGIFYGTSQKSPSSFGIASIDQKENFINKDNLDYQKARPSIKDSNLKEIPKPKPQPKPKPQPTPFPDPIPTPPKKEELKKPDIKPEEPKKPEIKPEPKPEPIPQPAPPIETKPKEELLPPNPPPPKEEPKPEPNPQPQPQQVPNNSNSRIIEINGVRVEAEVEVPPPRDIAEYDKQNNLVNPNPYINDSVGKIKNVKVTDELRKATGKLVQGNLGR", "aa_hexdigest": "daaa1aa9b0b4f871daeb346dca705889", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26798.55, "isoelectric_point": 9.439605903625488 }, "id": "GPKLONFDEH_118", "locus": "GCA000006625_00078" }, { "type": "cds", "sequence": "contig_1", "start": 48754, "stop": 48984, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTCTAAATTTGTTTCAAAAGACCATAAATATGCATGGCTTTATCAACATTTAGACTTAACAAAGTTTACACAATTATCAAATGAATCAGAATCTTATTTAAAAGAAGGCTATACACCTGATCCTGATAATGCATATGTTGATGAAAATGGAAAAATTAGTTCTCACGCATATTCACCTGCCAAAGGTTATAATTCGGTAACAAGTCGAATGGAAAATGATAATTGA", "aa": "MKSKFVSKDHKYAWLYQHLDLTKFTQLSNESESYLKEGYTPDPDNAYVDENGKISSHAYSPAKGYNSVTSRMENDN", "aa_hexdigest": "296006dccc7dc03af630901c9f4ff0d8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8771.4659, "isoelectric_point": 5.828179359436036 }, "id": "GPKLONFDEH_119", "locus": "GCA000006625_00079" }, { "type": "cds", "sequence": "contig_1", "start": 49288, "stop": 49473, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGAAATCAGATTCAAGTCAAAAATTTGTTGATATTCTAAAAACATTACCAAATCAATTGCCTTTATTAGAATTGTTTTTCTCCGCTGGTTCGCATAATACCTCTTCATTAATCGCACTGAAGGATAAAAAAATTAAAGAATTAGGTTTGTTCACACTAGGAAATTCATTATTAGATGAATGA", "aa": "MGKSDSSQKFVDILKTLPNQLPLLELFFSAGSHNTSSLIALKDKKIKELGLFTLGNSLLDE", "aa_hexdigest": "8d715ff41db20117057110802f6c0d87", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6692.683799999998, "isoelectric_point": 6.549522972106933 }, "id": "GPKLONFDEH_120", "locus": "GCA000006625_00080" }, { "type": "cds", "sequence": "contig_1", "start": 49903, "stop": 50199, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTATTGAGGACTTAAATGAAGCCGGATTTAACGAACATATTGTAAGTGGTGAACCTGGTGAAAAAAGTAAAATTACTTTTAGCAATGGTTCTGGTACAACTAAAATCCAAATTGATGGTGATCAAGAATTGTCAGCAAATGGAATTAGTAACTTATCAGCAATGTTTAATTTTGCTGAAAGTTTACAACGGACAATTGTTGTAAATAATACCAACTCACCACTTGCAAATCAACTAAGAAATGCGGGATATAGTGTTGAATCAACAACTAACGCTGGACTAATAGATATTTAA", "aa": "MSIEDLNEAGFNEHIVSGEPGEKSKITFSNGSGTTKIQIDGDQELSANGISNLSAMFNFAESLQRTIVVNNTNSPLANQLRNAGYSVESTTNAGLIDI", "aa_hexdigest": "db79684369f4fd386d4bd43f63c8de28", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10406.250799999989, "isoelectric_point": 4.336383247375489 }, "id": "GPKLONFDEH_121", "locus": "GCA000006625_00081" }, { "type": "cds", "sequence": "contig_1", "start": 50230, "stop": 51105, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04200.17" ], "nt": "ATGAAAAAAAATAAGTTAACTACTCTCGCTTTAATATTACCAATTACAATTTTAACTCCTATTGTTATTGCCTCATGTACAAATAAAACTAAAGTTAAAAAATCATCATCTTTAGATAAGATTGCTTCAAATTTAAAATTAGAGTATTTTAATAACAAAGCTAATACTAAAGCATCAAGTGTGCAAAAAGATGAAATTAAAAAACCACTAAATTTACCAAATGATGTTGTTTTTAGTGTTAAAGACGTTTTTGTTAGTCACAAAGATCAAAGTGTATTAATTGTCAAATACACTTTAAAAAAAGGGAATGAAATTCAAGAGTATACTTATGAAATTAAAGGTTTTAAATCTGTTTATGAAAAAGATAAAATCGTTAATGATTTAAGTCAAGCAAACGAAGATTTTAAAAAAATTGTTAATAATATCAGGTTAAAAGATACATTTGATTTTAAACTAGCAGCTTTTCCTAATCAAAATTATGACCAATTATTACCATCACAAATTTATAAAAATTATTATCAAGGTATTGAAATTCAACAACACAAATATCAAAATGAATTAGATATTAAAATTATTAATTTTTTATATCCTGATGGTGATTTTGGTTCGGCAAATAAAAATGGTACTTTAAAATTATCATTAATGTTAACTGATAAAAAAAATAATCAAGTTTACTATAAACTTTTAGAGGTTTCTGGCTTTAAATCTAATCCCTATGGTGTTGATGAAAATGGAACAATTCCTGGAATTGGTACAGAACGTTTAAAACCTAAAAATCAAGACGATTATTTTTCAAAAACTCAATTGCAACGTTATGAAATTGATAATGAAGGATATCTTCAAATTTTAAAACGCCAAAATAATGATAAAAATTGA", "aa": "MKKNKLTTLALILPITILTPIVIASCTNKTKVKKSSSLDKIASNLKLEYFNNKANTKASSVQKDEIKKPLNLPNDVVFSVKDVFVSHKDQSVLIVKYTLKKGNEIQEYTYEIKGFKSVYEKDKIVNDLSQANEDFKKIVNNIRLKDTFDFKLAAFPNQNYDQLLPSQIYKNYYQGIEIQQHKYQNELDIKIINFLYPDGDFGSANKNGTLKLSLMLTDKKNNQVYYKLLEVSGFKSNPYGVDENGTIPGIGTERLKPKNQDDYFSKTQLQRYEIDNEGYLQILKRQNNDKN", "aa_hexdigest": "e275eae927ca74b987174e8556dfe92e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 85, "aa_cov": 0.27491408934707906, "hmm_cov": 0.9764705882352941, "evalue": 8.968317888661248e-20, "score": 66.4494857788086, "start": 38, "stop": 117 } ], "seq_stats": { "molecular_weight": 33523.891500000005, "isoelectric_point": 9.283270072937015 }, "id": "GPKLONFDEH_122", "locus": "GCA000006625_00082" }, { "type": "cds", "sequence": "contig_1", "start": 51112, "stop": 51297, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCCCTGATTTAAATGCAACTGTTAGTGATATTAAACATTTTGATGAAAAAGCAAAAAACGTGGGTCAAGATTCTTATGAATCTGCTGCTTATAAAGGTTTTACATTACCTGTTTATGAAAGTGATGGCAAAATCAGTGGTTTAGCATTAGCAGGTAAAGATACACCAAAAGGGCCGTCATGA", "aa": "MRPDLNATVSDIKHFDEKAKNVGQDSYESAAYKGFTLPVYESDGKISGLALAGKDTPKGPS", "aa_hexdigest": "f345a6cfbd6793273f40c46a7e0f885b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6517.160500000002, "isoelectric_point": 5.628390312194824 }, "id": "GPKLONFDEH_123", "locus": "GCA000006625_00083" }, { "type": "cds", "sequence": "contig_1", "start": 51349, "stop": 51459, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAATGAAAAATATCGACAAGAAGCAATGCAAACATTTAGTTTAGGAATTTTAAATAACGATTCGCACAAGAACAATACTTATAATAAAACTGCAGGTACAACATGA", "aa": "MPNEKYRQEAMQTFSLGILNNDSHKNNTYNKTAGTT", "aa_hexdigest": "7f8453bea47e8aada05645d637e6f22e", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4118.479300000002, "isoelectric_point": 8.161197853088382 }, "id": "GPKLONFDEH_124", "locus": "GCA000006625_00084" }, { "type": "cds", "sequence": "contig_1", "start": 51571, "stop": 51846, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGCGATTAATGGATTCTGCACAAATTAAAACTACTTTTAGATTAAGTAATTTAGATGAGAATATTTATAATTTTGGTTTTCGAAGTAAAGAACATGGCAAAAATCTTTTAAACCATGGACTTAAAAAAATTTTTGATGGCCGTGATTTTTTAAAAACAAAACCGGCTGAATATTTGATTAATTCACAAAAAGAAAAATATAAAGATGTTGGTAATTTTACAGATTTTGCTGTTTTTGAATTAGATTTTGAAAAATTAGAATTAGTTAATGTTTGA", "aa": "MRLMDSAQIKTTFRLSNLDENIYNFGFRSKEHGKNLLNHGLKKIFDGRDFLKTKPAEYLINSQKEKYKDVGNFTDFAVFELDFEKLELVNV", "aa_hexdigest": "db191f73479d1d5de5a570ec21e27b8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 87, "aa_cov": 0.9340659340659341, "hmm_cov": 0.9195402298850575, "evalue": 1.0096671076308396e-17, "score": 60.40694046020508, "start": 2, "stop": 86 } ], "seq_stats": { "molecular_weight": 10724.114999999993, "isoelectric_point": 7.952191352844237 }, "id": "GPKLONFDEH_125", "locus": "GCA000006625_00085" }, { "type": "cds", "sequence": "contig_1", "start": 51910, "stop": 52044, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAAAGTCATTACAAGTAATTATGCTAATAATAAAAATAACCAGATTAAATTCTTATCTAAATCATATTTAAGTGATTATTCTAAAATTGATGTTCCTTTAAAATATAGACAAGAAGACGCTAAAACATGA", "aa": "MAKVITSNYANNKNNQIKFLSKSYLSDYSKIDVPLKYRQEDAKT", "aa_hexdigest": "ddf295cd4fdfb96e6189deef5b52324a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5128.768100000002, "isoelectric_point": 9.573829078674315 }, "id": "GPKLONFDEH_126", "locus": "GCA000006625_00086" }, { "type": "cds", "sequence": "contig_1", "start": 52384, "stop": 52734, "strand": "+", "frame": 1, "gene": null, "product": "MIP family Ig-specific serine endopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E090D9" ], "nt": "ATGGGACTAGAGTATTTACCCAAACATTTTGCACCAGCAGGTGGTGCTTCTGGAACAAGTGTACGCAATCAAAAAAATGAATTGGTTGCTATCTATCATGCTAAATATGATTCATCAAAAACAGGTCTAGCTGCTGCTTTTCGATCTGAAGGTTATGATTATCAAGGATTATATGGAAATTATAATTTACCACAATATGATTTAATTTATGGCGGTGGAAAAGATCAAACCGAAAAGAAATCATACCGAGAAGCGATGAAGGATATATATCAAAATAACAATATTAAAACAGCATTATTTCCTGATGGATTCGATAAGATTCCTGATGAATTCAAATTTAATAATAATTAA", "aa": "MGLEYLPKHFAPAGGASGTSVRNQKNELVAIYHAKYDSSKTGLAAAFRSEGYDYQGLYGNYNLPQYDLIYGGGKDQTEKKSYREAMKDIYQNNNIKTALFPDGFDKIPDEFKFNNN", "aa_hexdigest": "665688d29ae7118b7c75f104c8c1c451", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021E090D9", "query_cov": 0.9137931034482759, "subject_cov": 0.8548387096774194, "identity": 0.66, "score": 149.0, "evalue": 3e-44, "valid": false, "product": "MIP family Ig-specific serine endopeptidase", "uniref50_id": "UniRef50_UPI0021E090D9" }, "pscc": { "uniref50_id": "UniRef50_UPI0021E090D9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E090D9" ], "product": "MIP family Ig-specific serine endopeptidase" }, "genes": [], "id": "GPKLONFDEH_127", "locus": "GCA000006625_00087" }, { "type": "cds", "sequence": "contig_1", "start": 52771, "stop": 53616, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTATTAAACGTAAAACAAAATTATTAACGATTACAATTGGTGCAGTTGCTGTGAGTTCAATTTTATTAGGAGGAATTTTTTATGGTACAAGTCAAAAAAGTCCTTCAAGTTTTGGTATTGCTTCTATTGATCAAAAAGAAAATTTTATTAATAAAGACAATTTAGATTACCAAAAAGCAAGACCATCAATTAAAGATAATAACTTAAAAGAAATTCCCAAACCAAAACCTCAGCCAAAACCAGAACCACAACCAACACCATTTCCAGATCCCATCCCAACACCTCCAAAAAAAGAAGAGTTAAAAAAACCAGAGATTAAACCAGAAGAGCCTAAAAAGCCTGAAATTAAACCAGAACCAATTCCTAAACCAAAACCTCAGCCTATTCCTCAACCAACACCTCCTGTTGAAACTAAACCAAAAGAAGAATTATTACCACCAAGCCCACCACCTCCAAAAGAAGAACCAAAACCAGAACCAGATCCACAACCACAACCACAACAAATTCCTAACCAAAGTACTGTTAGAAAAATTGAATTGAATGGTGTTTTAGTGGACGCTGAAGTTGAAGTTCCACCCCCTCGTCAAACTTTTAAATATGATCAGGATAATGGATTATCAAATCTTAATCCCTATACAAATATAAGTGTTGGGAAAATTAAAAAAGTTTTTGTAACAGATGAGTTGCGGCACAAATCAGCAGATTTAGTGCGCGGGAATTTAAAGCGTGGCGATTATCAAAGTTTAGTTAAAGATTTATTAGATCCAAATATAAAACCAGAGGAAATTGATAGTTATATCGCAATGGTTGATAAAAGTGGATATCACGCTAAATTATGA", "aa": "MKFIKRKTKLLTITIGAVAVSSILLGGIFYGTSQKSPSSFGIASIDQKENFINKDNLDYQKARPSIKDNNLKEIPKPKPQPKPEPQPTPFPDPIPTPPKKEELKKPEIKPEEPKKPEIKPEPIPKPKPQPIPQPTPPVETKPKEELLPPSPPPPKEEPKPEPDPQPQPQQIPNQSTVRKIELNGVLVDAEVEVPPPRQTFKYDQDNGLSNLNPYTNISVGKIKKVFVTDELRHKSADLVRGNLKRGDYQSLVKDLLDPNIKPEEIDSYIAMVDKSGYHAKL", "aa_hexdigest": "e21cb4f4292f7f9f0b6f8bf0bd4b057c", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31479.909499999987, "isoelectric_point": 9.186889839172363 }, "id": "GPKLONFDEH_128", "locus": "GCA000006625_00088" }, { "type": "cds", "sequence": "contig_1", "start": 54229, "stop": 54414, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGAAATCAGATTCAGGACAAAAGTTCTCTGATATTCTAAAAGCCTTACCAAATGAATTGCCATTGCTTGAATTGTTCTTTTCTGCTGGTTCACATAATACGTCAGCACTTTCTGCTCTTGAAACTAAACATATTAAAGAACTTGGGTTGTATACATTAGGTAATTCATTATTAGATGAATGA", "aa": "MGKSDSGQKFSDILKALPNELPLLELFFSAGSHNTSALSALETKHIKELGLYTLGNSLLDE", "aa_hexdigest": "60e10f4dcb7edc0a8b275996915f0ac2", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6591.407799999998, "isoelectric_point": 5.171461677551267 }, "id": "GPKLONFDEH_129", "locus": "GCA000006625_00089" }, { "type": "cds", "sequence": "contig_1", "start": 54688, "stop": 55146, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAAATAGTTATCCCCAAGGTTTAGACTTTAGTCGTGTCCCTCAAATTCGCTCATTACGGGGTTTAATATTTAAAGACGAACAAAAAGCTTCAAACAATCGTGAACGTAAATTAAGAAGAGTTAATTTCTTTAATGATAAAGAAAATTATGAAATGTCAATTAATGATTTAAATGAAGCTGGTTTTAGTGAACATATTGTGACTAATGAACCAATGCCTCCTAAAAGTAAAATTACTTTTAGTAATGGTAATGCTACAAAACGTATTTACATTAAAGGCAATGGTAGTCTAACTGCAAGTGGTATTCAAAATTTAGCTACATTATTTAATCTAGCAGAAAGTTTAGATTCTAAATCTGTTGTTGTTGATTCTAATAATTCAGAATTAAAGTCACAATTAGAAGGTTTAGGATATAAAGTAAGTGATGCTTCTGACGCTAACTATATTGATATTTAA", "aa": "MGNSYPQGLDFSRVPQIRSLRGLIFKDEQKASNNRERKLRRVNFFNDKENYEMSINDLNEAGFSEHIVTNEPMPPKSKITFSNGNATKRIYIKGNGSLTASGIQNLATLFNLAESLDSKSVVVDSNNSELKSQLEGLGYKVSDASDANYIDI", "aa_hexdigest": "ddbc3f4adc5ee1667260f88898d309fe", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16955.687799999985, "isoelectric_point": 6.598859214782716 }, "id": "GPKLONFDEH_130", "locus": "GCA000006625_00090" }, { "type": "cds", "sequence": "contig_1", "start": 55161, "stop": 56147, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAAAAACTTATTGCAAGCGTTAGTATAATCACACCAATTGTGTTTGTTTCAGCTTTAGCAGCGTCTTGTGTTAATAATAAACATGAAGATAAAAATATTGTGCATAATAGTAGTGACCATGGTAATAATAAAAAAAATAATTTTACTAATGATTTAAATCAAAATTTAACAACCCACGAATTTAAATCAAATTTAATAAGTACTTCCCAATTAGATAATATAGCCAAATTAATTAATTTTACTTATGAAAATAAAGCTAATACTTATTTAAAAAATATACTTATTAATCAATTAAAACATTCACCTATTCAAAATCAAGATTTTAAACTAGAAATTATAGGTTTATATCCTAAACAAAATTCCTTGCAGGATTTAATTATTTATTATAAGTTAACTAACCAAAAAACAAAGGAGATTAAAGGTTATTATTTTGAATTAAATGGTTTTAAAAAACCAGATAACACCATTTTTTCAAATCAATTAACGCCTGAGCTAAAAAAAATTATTGATACTATTCAATTCAAAAAAACTTTTGATCTCGTAATTAATAATCAACAAGTATTAAATTATGAAAATGTTTTACCTTCACAAATTAAAAATCAATTATTAATGGGTTTAAAAGTTATTCAAAAAGAGTATACAGACAAAATTAAACTAAGTGTACTTGATGTTTTATTCATTAACGAGGGTGGGCAAATTAACGCTAATAAGTTGGGGGCTTTTTCTTTATTATTAGAGGTTTTAGATCTTAAAAGTAAAAAAACTTACCAAATTCTTATTCCTGTTGATAAATTTAAAACTAATCCTTATGGTGCTGATGAATATGATTTATTGCCAACGCAAGTTAATAACGGATTTGCACCAACTAGCTTAGCTCAAATTAATCAATACAATAATGCTGATCAACAAACACGTTATTTATATGATAATGAAAATTATTTAAAATCATTAAAAGCATATCAAAGAAATGTAAATTGA", "aa": "MKIKKLIASVSIITPIVFVSALAASCVNNKHEDKNIVHNSSDHGNNKKNNFTNDLNQNLTTHEFKSNLISTSQLDNIAKLINFTYENKANTYLKNILINQLKHSPIQNQDFKLEIIGLYPKQNSLQDLIIYYKLTNQKTKEIKGYYFELNGFKKPDNTIFSNQLTPELKKIIDTIQFKKTFDLVINNQQVLNYENVLPSQIKNQLLMGLKVIQKEYTDKIKLSVLDVLFINEGGQINANKLGAFSLLLEVLDLKSKKTYQILIPVDKFKTNPYGADEYDLLPTQVNNGFAPTSLAQINQYNNADQQTRYLYDNENYLKSLKAYQRNVN", "aa_hexdigest": "7829d2447d660140864a35ccca6d94ba", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37694.6596, "isoelectric_point": 9.200363731384279 }, "id": "GPKLONFDEH_131", "locus": "GCA000006625_00091" }, { "type": "cds", "sequence": "contig_1", "start": 56379, "stop": 56498, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAGAACTTTGCCTAATGATATGTATAAGCAAATTGCTTTACAAACATTTGGCATTCAAATTCAAACGCCAAATGGTAAACCACGCGAATCTGATGTAGTAGCTGGAACAATGTGA", "aa": "MARTLPNDMYKQIALQTFGIQIQTPNGKPRESDVVAGTM", "aa_hexdigest": "1340aa78c7d69c1a301ccb966323a53c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4321.953200000003, "isoelectric_point": 8.25325870513916 }, "id": "GPKLONFDEH_132", "locus": "GCA000006625_00092" }, { "type": "cds", "sequence": "contig_1", "start": 56502, "stop": 56921, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGGATTATCAAAAAAGAAATGATAATAAATATCCTACTAAATGGTATTTTGGCACAAATTTACATGTTGCAGAAGCATTAAAGTCAACAACAACAGTTTTTGGTATTAATAAGATAATGCCAACAGTTAAGACTAAAACTACTTTGGGTTTAGCGAACGCTGATGATAATATTTATCGTTTTAGTTTAGTTAGTAAAGATAATCAACCAAATATGGATAAACCTATTAGTAATGGTATTAAAACAATCTATGATGGTCGTGACTTCTTAAAATTAAATCCTAGTGACTTATTAACATCAAAATTAAAAAATAAATACCATAACCTACAAGAATTTGTTGATTTTGCTGTTTTTGAAATTGATTTTGAAAAAATAAAACTCGGCTCTATAACGAAAAATTTTTATAGTGGATCAGATTGA", "aa": "MDYQKRNDNKYPTKWYFGTNLHVAEALKSTTTVFGINKIMPTVKTKTTLGLANADDNIYRFSLVSKDNQPNMDKPISNGIKTIYDGRDFLKLNPSDLLTSKLKNKYHNLQEFVDFAVFEIDFEKIKLGSITKNFYSGSD", "aa_hexdigest": "2ce66634d9ac1421a719d33b07bbccf7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 145, "aa_cov": 0.9064748201438849, "hmm_cov": 0.9310344827586207, "evalue": 8.529204096927635e-31, "score": 103.39796447753906, "start": 1, "stop": 126 } ], "seq_stats": { "molecular_weight": 15959.943499999985, "isoelectric_point": 9.155622673034667 }, "id": "GPKLONFDEH_133", "locus": "GCA000006625_00093" }, { "type": "cds", "sequence": "contig_1", "start": 57060, "stop": 57209, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTCAAAATACAATCAGGCTTTTGACACAAATAAATATGATGAATTATATGCTGTAGGTTGGCCAAGCTCTGCTTCTGATTATTTCTTAGACCCAATTAAAGATCAGAAACAATTTGAATCAAGACGTGAAAGTTATAGTTTATGA", "aa": "MDSKYNQAFDTNKYDELYAVGWPSSASDYFLDPIKDQKQFESRRESYSL", "aa_hexdigest": "12c003be40d8d66d56290ef5647cdbc6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5835.2501999999995, "isoelectric_point": 4.518097496032715 }, "id": "GPKLONFDEH_134", "locus": "GCA000006625_00094" }, { "type": "cds", "sequence": "contig_1", "start": 57258, "stop": 57764, "strand": "+", "frame": 3, "gene": null, "product": "MIP family Ig-specific serine endopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "nt": "TTGTTTCCTCTTACACAAATAAATCGTGGTGATTTCTTGTCATATAATATTGGTTATCGTAGTTTTACTAATAAACCTGGATTGCTTGATGCTTTTATTGCTAGTCCTATTACTGGTAAAACTGTACATAAATCAACTGATAATCAAAATTATATTGGGTTTGGTTTAAATTATTTACCGATGCATTATTCTCCAATTGGGGGTTCATCAGGGACAAGTCTACGTAATCAAAAAAATGAATTAGTGGGAATTTGGCATGTTGGTAATGGTTTTGCCCAAACAGGTTTGGCTGCTGCTTTTCGATCTGAAGGCTATGATTATCATGGATTATATGGTAGTTATAACTTACCACAATATGATTTGATTTATGGTGGTGGAAAAGATCAAAAAACTTCCTATCGCCAAGCAATGATGGCGTTATATAAAAATAAAAATATTGCAACCGCTTTATTCCCTAATGGTTTTGGTGAAGATCAAATCCCCAGTGCTTTTAAATTTAAAAATTAA", "aa": "MFPLTQINRGDFLSYNIGYRSFTNKPGLLDAFIASPITGKTVHKSTDNQNYIGFGLNYLPMHYSPIGGSSGTSLRNQKNELVGIWHVGNGFAQTGLAAAFRSEGYDYHGLYGSYNLPQYDLIYGGGKDQKTSYRQAMMALYKNKNIATALFPNGFGEDQIPSAFKFKN", "aa_hexdigest": "efbe96d508f148e4ee720b29783b1ae2", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AFE92B4", "query_cov": 0.9107142857142857, "subject_cov": 0.9444444444444444, "identity": 0.601, "score": 200.0, "evalue": 4.41e-63, "valid": false, "product": "MIP family Ig-specific serine endopeptidase", "uniref50_id": "UniRef50_UPI0021DFAD80" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFAD80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "product": "MIP family Ig-specific serine endopeptidase" }, "genes": [], "id": "GPKLONFDEH_135", "locus": "GCA000006625_00095" }, { "type": "cds", "sequence": "contig_1", "start": 57794, "stop": 58177, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAATACAAAAACATTTACTTCCGTTAATAGAGTAATTTATGATGATAACTATTCGCTAAAGCAACAACAAAAAAGTAGCTTTATCAATCAATTTTTAAAAGGATTGTTATCTGCTATTACTTTATTATTCTTTATTTTACTTTTAATCTTTGCTGAAAATACGTTGTTTGGTTTAGGTTTTGGTGATGAGAATAAATCAATGATGATTTCAAAATCGCTAAATGCTTTTTTTGATTTACACAGCCCTAAATATTTACAACTTAACTTTTTAATTGTATTTCGCTTTTTTATTTTAAGTTTTACACTGTTTTATACCTTAATCAAAAATTTCACAAATCTTTATTGGCATCGAGCAACCATTAAAAAATACTTACCATGA", "aa": "MMNTKTFTSVNRVIYDDNYSLKQQQKSSFINQFLKGLLSAITLLFFILLLIFAENTLFGLGFGDENKSMMISKSLNAFFDLHSPKYLQLNFLIVFRFFILSFTLFYTLIKNFTNLYWHRATIKKYLP", "aa_hexdigest": "1159ee97a2bb1e6c288711f7de9e1ea9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15027.54519999998, "isoelectric_point": 9.776324272155762 }, "id": "GPKLONFDEH_136", "locus": "GCA000006625_00096" }, { "type": "cds", "sequence": "contig_1", "start": 58805, "stop": 59284, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTCACTTTAAAAAAACGCTTAAGCGTAAAGAGTAGTTTAAATACTTTATTAAATTTAGTAGTCACTCAAACAATTCTAAGTTTAAGCTTGTTTTTAGTAACATTATTTAATTCTAAGAGTGTGGTTTCTTTAATTAATGTTTTTATTACAATTACTGTACAAATGAGTGTGTTTGGAATCTACATATTTCAAAATAAAAACATTTCAACTAAATTATTGGTGCTATTAAAGGTTATTATGATTTTAATTATTTTAACCGCAGCAATTGTAGGTTTTGATTATTTATTAACATCTGATCATCATAATAACTATTTATTCTCAAACATTCAACCGAAAATGAATTTAGTACAAATCATGTTGTTATTAAATTTTAGTTTGAATTTTACTTTAATTAGTTATTTAACAATTAAATTTGCAATGGTAATTTTTAAAATCAATAAGCTAAATAAGGAGTTAAACAATGAAAAAAAATAA", "aa": "MVFTLKKRLSVKSSLNTLLNLVVTQTILSLSLFLVTLFNSKSVVSLINVFITITVQMSVFGIYIFQNKNISTKLLVLLKVIMILIILTAAIVGFDYLLTSDHHNNYLFSNIQPKMNLVQIMLLLNFSLNFTLISYLTIKFAMVIFKINKLNKELNNEKK", "aa_hexdigest": "a87587faf9da5ff63736bb050b54d008", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18178.846900000008, "isoelectric_point": 10.095829582214353 }, "id": "GPKLONFDEH_137", "locus": "GCA000006625_00097" }, { "type": "cds", "sequence": "contig_1", "start": 59271, "stop": 59483, "strand": "+", "frame": 3, "gene": null, "product": "putative protein UU049", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR96", "UniRef:UniRef90_Q9PR96" ], "nt": "ATGAAAAAAAATAAATCTAATCTGACACCTACAACAAATTATTTTGATGTATTTAATACTTATAAAGAGAAAAAAGCAAGTGTTGATTTAATTACATATGAAGAATTAATGGCATCAGTTTTATTTGATAATAAATTAGGTTTTGAATCAGAGGTTTATTTAGATTTTGTTAAAAAATTTACATTAGCTTTTGAAAAAAAACTAGATATTTGA", "aa": "MKKNKSNLTPTTNYFDVFNTYKEKKASVDLITYEELMASVLFDNKLGFESEVYLDFVKKFTLAFEKKLDI", "aa_hexdigest": "09f2186cc324674883ee130d6d4f00a0", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 59271, "stop": 59786, "observations": { "mutation": [ 59481 ], "stop": [ 59481 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR96", "query_cov": 1.0, "subject_cov": 0.4093567251461988, "identity": 1.0, "score": 138.0, "evalue": 3.46e-40, "gene_start": 1, "gene_end": 70, "reference_start": 1, "reference_end": 70, "reference_sequence": "MKKNKSNLTPTTNYFDVFNTYKEKKASVDLITYEELMASVLFDNKLGFESEVYLDFVKKFTLAFEKKLDIWFENFIINFNLNLKFSTTIMIPILVTKANSTTDAINFRNDQNPVYNNFLISYNQKIKKLLLQNHPVQILPHLILFKSNLNGSLVLVFSEKIIASIEQKSGN" }, "paralog": false, "identity": 0.9941520467836257, "score": 333.0, "evalue": 8.33e-120, "description": "Internal stop codon at 59481. Nonsense mutation around 59481.", "psc": { "uniref90_id": "UniRef90_Q9PR96", "product": "Uncharacterized protein UU049", "uniref50_id": "UniRef50_Q9PR96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR96", "UniRef:UniRef50_Q9PR96" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR96" ], "product": "Uncharacterized protein UU049" } }, "id": "GPKLONFDEH_138", "locus": "GCA000006625_00098" }, { "type": "cds", "sequence": "contig_1", "start": 59538, "stop": 59786, "strand": "+", "frame": 3, "gene": null, "product": "putative protein UU049", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR96", "UniRef:UniRef90_Q9PR96" ], "nt": "ATGATTCCAATATTAGTAACTAAAGCTAATTCTACAACTGATGCAATTAATTTTAGAAATGATCAAAATCCTGTTTATAATAATTTTTTAATTTCATATAACCAAAAAATTAAAAAATTATTACTTCAAAATCATCCTGTTCAAATTCTTCCTCATTTGATTTTATTTAAATCAAATCTTAATGGTAGTTTAGTGCTCGTATTTAGTGAAAAAATTATCGCTTCAATTGAACAAAAATCAGGAAATTAA", "aa": "MIPILVTKANSTTDAINFRNDQNPVYNNFLISYNQKIKKLLLQNHPVQILPHLILFKSNLNGSLVLVFSEKIIASIEQKSGN", "aa_hexdigest": "29ced18b53ff2d06fb560b1a4884a385", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 59271, "stop": 59786, "observations": { "mutation": [ 59481 ], "stop": [ 59481 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR96", "query_cov": 1.0, "subject_cov": 0.47953216374269003, "identity": 1.0, "score": 157.0, "evalue": 1.02e-47, "gene_start": 1, "gene_end": 82, "reference_start": 90, "reference_end": 171, "reference_sequence": "MKKNKSNLTPTTNYFDVFNTYKEKKASVDLITYEELMASVLFDNKLGFESEVYLDFVKKFTLAFEKKLDIWFENFIINFNLNLKFSTTIMIPILVTKANSTTDAINFRNDQNPVYNNFLISYNQKIKKLLLQNHPVQILPHLILFKSNLNGSLVLVFSEKIIASIEQKSGN" }, "paralog": false, "identity": 0.9941520467836257, "score": 333.0, "evalue": 1.31e-119, "description": "Internal stop codon at 59481. Nonsense mutation around 59481.", "psc": { "uniref90_id": "UniRef90_Q9PR96", "product": "Uncharacterized protein UU049", "uniref50_id": "UniRef50_Q9PR96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR96", "UniRef:UniRef50_Q9PR96" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR96" ], "product": "Uncharacterized protein UU049" } }, "id": "GPKLONFDEH_139", "locus": "GCA000006625_00099" }, { "type": "cds", "sequence": "contig_1", "start": 59789, "stop": 60703, "strand": "+", "frame": 2, "gene": null, "product": "MSC_0622 family F1-like ATPase gamma subunit", "db_xrefs": [ "RefSeq:WP_006688472.1", "SO:0001217", "UniParc:UPI0000139197", "UniRef:UniRef100_Q9PR95", "UniRef:UniRef50_Q9PR95", "UniRef:UniRef90_Q9PR95" ], "nt": "ATGCAATTAAAAGATTTAATTAATAAAAAAAAGAATTTAAATAATATTAATTTAAAAGTGAGTAACGAACGTAATATTTTTTTAATTAATATTATGAAACTAAATCAGCGATTAACTTTTTTTAGTAAAAATGCTTTTGAAATAAAAGAATCAATTCTAAGTTTAAAGCGTATTTATAATATTAAACATGATATGTTAAGACATGAGGAACGAAAGATTTTTAAGTTTTTAAATAAAATTAATGATCGTGTTTTATGGATTTATTTAACTGAAGAGCAAAAATATAGTACTGATTCATATTCGCGTTATGAACAAAAAATCTTAAAAACGATTAAATCAAATCGTGATGATTTTATTTTGATCGGTCAAGGAGCAATTGAATTTGGTAAGAATCATAATTTAAATGTTCTGCAAACATTTAATGACTCAAATATTAAAAATTTAACTACACAATTAACTAAAATGATTATGATTTTATATACTTTTGATAATTATAAAAAAGTTAATTTTGTTATTAATTCTAATAAAAACTATGATGGACACTTTACTATATTACCAATGAATGAATTTAGTTTTGATAAATTTATTAATTTACAAAAATGCGATTCTAATATTATTGATTTTCAAAAAGTAAAAATTTATCCAAATTTAAATGAATTTATTAATGTCCAAATTAATGTTTTCTTAGTTAATATTATTAATACTTTAATTACTGAATCATCTTTTTATAAAACAAAAAATGGACTAGTAGCTACTAATAATATCCTAAAAGAACTAGATGATAACCTTTCAAAAATTCAACGTAAAATTACACGTGTAAAGACAGAGTTGCAAATTGAAGAAATAAATTTATTAGCAAGACAAAATATGAATGAAGATGATAATGATAACGATGGTGGTGTTTATGAATCATAA", "aa": "MQLKDLINKKKNLNNINLKVSNERNIFLINIMKLNQRLTFFSKNAFEIKESILSLKRIYNIKHDMLRHEERKIFKFLNKINDRVLWIYLTEEQKYSTDSYSRYEQKILKTIKSNRDDFILIGQGAIEFGKNHNLNVLQTFNDSNIKNLTTQLTKMIMILYTFDNYKKVNFVINSNKNYDGHFTILPMNEFSFDKFINLQKCDSNIIDFQKVKIYPNLNEFINVQINVFLVNIINTLITESSFYKTKNGLVATNNILKELDDNLSKIQRKITRVKTELQIEEINLLARQNMNEDDNDNDGGVYES", "aa_hexdigest": "c8d5dda3918a4bfc15fd5022402939ad", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000139197", "ncbi_nrp_id": "WP_006688472.1", "uniref100_id": "UniRef100_Q9PR95", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139197", "RefSeq:WP_006688472.1", "UniRef:UniRef100_Q9PR95" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR95", "uniref90_id": "UniRef90_Q9PR95" }, "psc": { "uniref90_id": "UniRef90_Q9PR95", "product": "MSC_0622 family F1-like ATPase gamma subunit", "uniref50_id": "UniRef50_Q9PR95" }, "pscc": { "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95" ], "product": "Uncharacterized protein UU050" }, "genes": [], "id": "GPKLONFDEH_140", "locus": "GCA000006625_00100" }, { "type": "cds", "sequence": "contig_1", "start": 60672, "stop": 60794, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACGATGGTGGTGTTTATGAATCATAATTATAATATTCAAATTACATTACTTGAAAACAAAAAGTTTAATATTGATAATGGTTTATTATATGTAAATGTTAATGAAGAAAATACATGA", "aa": "MITMVVFMNHNYNIQITLLENKKFNIDNGLLYVNVNEENT", "aa_hexdigest": "2ab2167f3ccc4ac95e20c6c6783d1c30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4745.411000000002, "isoelectric_point": 4.830996131896971 }, "id": "GPKLONFDEH_141", "locus": "GCA000006625_00101" }, { "type": "cds", "sequence": "contig_1", "start": 60999, "stop": 61172, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGATAAATATAAAAATATTACTAATAATATTTTAGAACTTGAAGCTAAACAACAATTAGGATTAACTCTAAGTGAATTTTTAGATCTTGATAATCTAAAACAAGAACAATATATAACTAATATGCAAATTAGATTAAATTTAGTGGAGTATAAAAAAGATGAGAAATAA", "aa": "MVDKYKNITNNILELEAKQQLGLTLSEFLDLDNLKQEQYITNMQIRLNLVEYKKDEK", "aa_hexdigest": "67df1e428446086b8e2527d75d5ee038", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6831.774300000001, "isoelectric_point": 4.927224540710449 }, "id": "GPKLONFDEH_142", "locus": "GCA000006625_00102" }, { "type": "cds", "sequence": "contig_1", "start": 61162, "stop": 61923, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATAAATTGAAAATAAAATTAAAATTTTTAGCTTCACTAGCAACAATACCAATTGTAGCTTCACCATTAATTTTTTCTGTTGCAGCTAATGAGGATGGTAAACAAGAAAACAATAATGGTAATAATCATGATCCTAATCAACAAAAAAAACCAAAAATTCCTAAAAACGATCCTAATTTTAATACTTTTAAAACTGATGCTGATAAAACTGTTAAAGATAGTTTAGAAAAAGGAATTAATGCTGCTATTGTTTATGTAAAATCACGTCAAGAAGAAATTTTAGAAAATAAAGAAATTGAGTTTAAGAAAAAAATTCAACAACTAATTTATTTAAAAAATCTGCAGTCATATCTTGAGAAAAATAAAGAAAACATCTTAAAAAATCCTAATGATTATGGTTTTTATTTAAATACTCCTCAAATTTTAGGAACTCTTAAAAATTATGATATTAAAGATATTGAGTTTAATGGAGAAACATATAAGCAAATCAAAGTTGGCAAAACTGATCCTTTAAATTATCAAAAAGCCGTTGCTCCAAAAGGAAAAATTAGTGATGTACAAAGTGATCAAATTAATGATGTTGAAGAAACAAAATACAAAGATACGTTAAAAAAATATGAATCTGAATTTTTAAAAGAAATTAATAAATTAATTTATGATGAAAATGATGTTCCACAAATTAACAAAGATGTTGAGTTAACACGTGATGAAAAAGGACAATTTAATACAACTTTACCTAAAGGTTATAATGATTGA", "aa": "MRNKLKIKLKFLASLATIPIVASPLIFSVAANEDGKQENNNGNNHDPNQQKKPKIPKNDPNFNTFKTDADKTVKDSLEKGINAAIVYVKSRQEEILENKEIEFKKKIQQLIYLKNLQSYLEKNKENILKNPNDYGFYLNTPQILGTLKNYDIKDIEFNGETYKQIKVGKTDPLNYQKAVAPKGKISDVQSDQINDVEETKYKDTLKKYESEFLKEINKLIYDENDVPQINKDVELTRDEKGQFNTTLPKGYND", "aa_hexdigest": "663c5652e33dc006c431a0e161a4c637", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29181.673200000023, "isoelectric_point": 8.616795921325686 }, "id": "GPKLONFDEH_143", "locus": "GCA000006625_00103" }, { "type": "cds", "sequence": "contig_1", "start": 62308, "stop": 62775, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATATTAAAGTTAAAATTATTGATCAAGTTAATCCTAAACTACAACGTACATATATTATTAATAAATATGATCCAATTTTAGATATTAATTTTAATAGTCTAAAATTAAATGAAGTTAATGCTATTAAACAAATTTTTGTTAACTTGTATAAACATTTAGGACTAGATGAGAAAATTGATTATAAGAAATTACGTAACTTTTATATTAGAAATGCTTTATTTACAATGATTGAAGCTGCTCAAAAACTTATTTTGCGATTCAATCAAATTGATAAAGATAATAAAATTATTGGTCAAAAAACTTTTGCAACATTACAAAATGAATATTTAGAAAAATACAAACAAAAACTTGTAAACAACACCGATGATAAGGAAAGATTATTAAATGAGTTTAATAATTTAACAAAAGAAAATTTCTTTCGTTATTTAAATAACACTTTAATCAATAATGATTATTATTGA", "aa": "MINIKVKIIDQVNPKLQRTYIINKYDPILDINFNSLKLNEVNAIKQIFVNLYKHLGLDEKIDYKKLRNFYIRNALFTMIEAAQKLILRFNQIDKDNKIIGQKTFATLQNEYLEKYKQKLVNNTDDKERLLNEFNNLTKENFFRYLNNTLINNDYY", "aa_hexdigest": "d60bf1994ef0ad7afbc0c1cb7f8704c1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18720.423400000007, "isoelectric_point": 9.415365791320802 }, "id": "GPKLONFDEH_144", "locus": "GCA000006625_00104" }, { "type": "cds", "sequence": "contig_1", "start": 63028, "stop": 63327, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTATATTAACAAATCAAACCGATATTAAAACAAATAAGGACAAAGCTAAGGATTTTAAAAATGCATATGATAGTGCTTTAAAATCTCTTGAAAGACAAAAACAAGTAAATAAACAAATTCGTCGTAAGATTGGAATTGCGTTTATAGTAATTAGCTTACTGGTTTTAATAATTAATTTAATTATTTATGGATTGATTAAAAAACTAAAAAATAAAAAAGTTATTCTAATTATTAATAGTGTGATAATGGTGTTGACAATCATTGTTTTAATAATGGGAATTATTCTAATAATTTAG", "aa": "MSILTNQTDIKTNKDKAKDFKNAYDSALKSLERQKQVNKQIRRKIGIAFIVISLLVLIINLIIYGLIKKLKNKKVILIINSVIMVLTIIVLIMGIILII", "aa_hexdigest": "178e6260af7948fd57d2ce8d66668bd6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11258.823399999987, "isoelectric_point": 10.334491539001462 }, "id": "GPKLONFDEH_145", "locus": "GCA000006625_00105" }, { "type": "cds", "sequence": "contig_1", "start": 63343, "stop": 63633, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACACTAAGGCAAACCCTCGTATTAAATCGATTTTTAACTATATTGTAACTGTTAGTGGAATCTATGATTATCAACATCATCAAATATTTGTTTTAAAAAATGATCCAACAGTAAAATTATTTGTTATTTCAGCAAAAGAAGATCTTGCTTATTTATTAATTAGCAATGAACATGCTAATATTAATATCGGCGATGAAATCATTGAAACAAATCGTGAAGAAAATGTTGTTACTGATTTAAATTATTTCGGGAAGATTATAGATATTAATAATAATTTTATTTGA", "aa": "MQNTKANPRIKSIFNYIVTVSGIYDYQHHQIFVLKNDPTVKLFVISAKEDLAYLLISNEHANINIGDEIIETNREENVVTDLNYFGKIIDINNNFI", "aa_hexdigest": "f363e1cf2717b0b7fc56c0eb68d3a22b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11100.450399999987, "isoelectric_point": 5.1671987533569315 }, "id": "GPKLONFDEH_146", "locus": "GCA000006625_00106" }, { "type": "cds", "sequence": "contig_1", "start": 63709, "stop": 64608, "strand": "+", "frame": 1, "gene": "atpA", "product": "MSC_0619 family F1-like ATPase alpha subunit", "db_xrefs": [ "COG:C", "COG:COG0056", "SO:0001217", "UniRef:UniRef50_A0A449A4K5", "UniRef:UniRef90_Q9PR92" ], "nt": "ATGACAGTGAAAACTTTAAATGAGCAATTAAATACTGGAATTGTAGCAATTGATTTATTAATTCCGATTGGCAAAGGACAAAGGGAATTAATTATTGGTGATCGTCAAACTGGAAAAACGCATATTGCTTTAAATACTATTATTAATCAAAGCCGTGCTGGCACAAAATGTATTTATGTAGCAATTGGTCAAAAACGCGAATCATTAACTACTGTTTATGAAACTTTAAAGGCTCATGACGCATTAAAAAACACAATTATTATCGATGCTCCTTTAACAGCATATGAACAATATTTAGCACCATATATTGGAATGGCTCATGCTGAAAACTTATCGTATCAAGATGATGTATTAATTGTATTTGATGATCTAACAAAACACGCTAATATTATTCGTGAAATGGCTTTATTAACAAATAAACCAGTTGGTAAAGAAGCAATGCCTTCAGATGTATTCTTCTCACATTCATCATTACTTGAACGTGCTGGTTCATTCAAAAATCGTAAAACAATTACTGCTTTGCCGATTTTACAAACAGTTGATGGGGATATTACTTCATTAATCTCTTCAAATATTATTTCTATTACTGATGGTCAAATTGTAACTTCAACTGATTTATTTGCTGCTGGTAAAGTTCCTGCTATTAATATTGATTTATCAGTTTCAAGAACAGGTTCAAGTGTACAATCAAGAATGATTACTAAAGTAGCAGGTGAAATTAATAAAATTTTTAGACAATACAAACGTCATTTAAAATTAGCAATGTTAGATTATGAATTTAATAAAGAAACTAGCCTTTTATTGCATAAAGGTAAATTAATAGATAAAATGTTTTTACAAAAAGGTTTCTCTTTATTTTCATACCGTTTTATTGTTTTAAGTACTAAGCTAATTGCCTGA", "aa": "MTVKTLNEQLNTGIVAIDLLIPIGKGQRELIIGDRQTGKTHIALNTIINQSRAGTKCIYVAIGQKRESLTTVYETLKAHDALKNTIIIDAPLTAYEQYLAPYIGMAHAENLSYQDDVLIVFDDLTKHANIIREMALLTNKPVGKEAMPSDVFFSHSSLLERAGSFKNRKTITALPILQTVDGDITSLISSNIISITDGQIVTSTDLFAAGKVPAINIDLSVSRTGSSVQSRMITKVAGEINKIFRQYKRHLKLAMLDYEFNKETSLLLHKGKLIDKMFLQKGFSLFSYRFIVLSTKLIA", "aa_hexdigest": "0d018a92c2fdd0be822aa5dc72f499e2", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpA" ], "truncated": "both", "pseudogene": { "start": 63409, "stop": 64878, "observations": { "mutation": [ 63631, 64606, 64795 ], "stop": [ 63631, 64606, 64795 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR92", "query_cov": 1.0, "subject_cov": 0.5851272015655578, "identity": 0.993, "score": 565.0, "evalue": 8.54e-200, "gene_start": 1, "gene_end": 299, "reference_start": 123, "reference_end": 421, "reference_sequence": "MQNTKANPRIKSIFNYIVTVSGIYDYQHHQIFVLKNDPTVKLFVISAKEDVAYLLISNEHANINIGDEIIETNREENVVTDLNYFGKIIDINNNFIWPKPLNNLPSAKIAQTNPAFGLAHNLMTVKTLNEQLNTGIVAIDLLIPIGKGQRELIIGDRQTGKTHIALNTIINQSRAGTKCIYVAIGQKRESLTTVYETLKAHDALKNTIIIDAPLTAYEQYLAPYIGMAHAENLSYQHDVLIVFDDLTKHANIIREMALLTNKPVGKEAMPSDVFFSHSSLLERAGSFKNRKTITALPILQTVDGDITSLISSNIISITDGQIVTSTDLFAAGKVPAINIDLSVSRTGSSVQSRMITKIAGEINKIFRQYKRHLKLAMLDYEFNKETSLLLHKGKLIDKMFLQKGFSLFSYRFIVLSTKLIAWGILKGVKDEQKAFMLLDYAINNYEDAQKAFNTISTTQNYDDKIMKNYFAFILKQYSDYLNLNWEVELEHSFIPLQNEFLMNVAKFLGDK" }, "paralog": false, "identity": 0.9877300613496932, "score": 940.0, "evalue": 0.0, "description": "Internal stop codon at 63631, 64606, 64795. Nonsense mutation around 63631, 64606, 64795.", "psc": { "uniref90_id": "UniRef90_Q9PR92", "gene": "AtpA", "product": "MSC_0619 family F1-like ATPase alpha subunit", "uniref50_id": "UniRef50_A0A449A4K5", "cog_id": "COG0056", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR92", "UniRef:UniRef50_A0A449A4K5", "COG:COG0056", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A4K5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A4K5" ], "product": "ATP synthase F0F1 subunit alpha" } }, "id": "GPKLONFDEH_147", "locus": "GCA000006625_00107" }, { "type": "cds", "sequence": "contig_1", "start": 64648, "stop": 64797, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTAGATTATGCGATTAATAATTATGAAGATGCTCAAAAAGCATTCAATACTATTTCAACAACTCAAAATTATGATGATAAAATTATGAAAAACTACTTTGCTTTCATCCTAAAACAATACTCTGATTACTTAAATTTAAATTGA", "aa": "MLLDYAINNYEDAQKAFNTISTTQNYDDKIMKNYFAFILKQYSDYLNLN", "aa_hexdigest": "ea148a5c6c834445f126bb93b84b1267", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5881.510200000001, "isoelectric_point": 4.460405921936036 }, "id": "GPKLONFDEH_148", "locus": "GCA000006625_00108" }, { "type": "cds", "sequence": "contig_1", "start": 65097, "stop": 66242, "strand": "+", "frame": 3, "gene": "atpD", "product": "MSC_0618 family F1-like ATPase beta subunit", "db_xrefs": [ "COG:C", "COG:COG0055", "SO:0001217", "UniRef:UniRef50_A0A449A4P1", "UniRef:UniRef90_Q9PR91" ], "nt": "ATGGTCCCAGTTGGTAATGATTCAAAAAATAATATTTATAATTTTTGGGGACATCCTTTATTAGAAACTAATAAAACACCTAAATATGTTGAAATGAATTCAACAATTTTAAACGAGCGATATGTTGATAAAAGTATAGAAATTGTAGAGACAGGAATTAAAGCAATTGATTTTTTTATGCCAATTCTAAAAGGATATAAATTAGGAATCTTTGGTGGTGCTGGAGTTGGTAAAACAGTTTTAATGAAAGAAATCATTTTTAACTTAAACAGACATAAACAAGCAAATTCAAATATTTTTATTGGTTCAGGAGAGCGTTCACGTGAAGCAATTGAATTATATAATGAATTGACTGCTTCTAATTTAATGCCTAATTCAGTAATGTTTATTTCTAAGATGAATGAAGCTCCTGGTGCACGTACTTCAATTGTACCAATTGGAATTACTGCTGCTGAATATTTACGTGATCAAAACAAAGAAAATGTTTTATTGTTTATTGATAATATTTATCGTTTTATTCAAGCTGAAAATGAAGTTTCTACTGCATTAGGTAAAAAACCGTCTGTTGGAGGTTATCAATCAACACTAGAAAGTGATGTTACACATATTCAAAATCGATTATTTAGAAATAAAAATGGATCAATTACCTCATTTCAAACAATTTTTTTGCCAATGGATGATTTAAGTGATCCATCAGCTGTCGCTGTATTTAATCATTTAGATGGAAAATTAGTATTATCGCGAGCACAAGCTGCTAAAAATATTTTTCCAGCATTTGATCCATTAGCAAGCTCAACAAACGCTATTGATCCTAAAATTATTGGTGAACGTCATTATAATGCAATTATCGAAACTAAAAAAGTTTTAAAAGCTTATAAAGATCTAGAAGATGTTATTTTAATTTTAGGTTTTGATGAACTAGATGCTGAAAGTAAAATTATTGTTAAAAAAGCTTTACAGCTAGAAATGTTCTTTACACAAAACTTTTTTATGACTGAACATTTCACAAAAGCTCCTGGTCAGTTTGTTCCATTAAAAGAGACTGTTGAAAGTGTAATTCGTATTTTAGAAGGTAAATATTTAAAACAAAGTCCAGATGTTTTTGCCTATATTGGTTCAAATAAGGATATTTCTCAAGATCATTAA", "aa": "MVPVGNDSKNNIYNFWGHPLLETNKTPKYVEMNSTILNERYVDKSIEIVETGIKAIDFFMPILKGYKLGIFGGAGVGKTVLMKEIIFNLNRHKQANSNIFIGSGERSREAIELYNELTASNLMPNSVMFISKMNEAPGARTSIVPIGITAAEYLRDQNKENVLLFIDNIYRFIQAENEVSTALGKKPSVGGYQSTLESDVTHIQNRLFRNKNGSITSFQTIFLPMDDLSDPSAVAVFNHLDGKLVLSRAQAAKNIFPAFDPLASSTNAIDPKIIGERHYNAIIETKKVLKAYKDLEDVILILGFDELDAESKIIVKKALQLEMFFTQNFFMTEHFTKAPGQFVPLKETVESVIRILEGKYLKQSPDVFAYIGSNKDISQDH", "aa_hexdigest": "4e2dcef1db19227d852b51c705f7db04", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR91", "query_cov": 1.0, "subject_cov": 0.8392070484581498, "identity": 0.95, "score": 717.0, "evalue": 2.25e-259, "valid": true, "gene": "AtpD", "product": "MSC_0618 family F1-like ATPase beta subunit", "uniref50_id": "UniRef50_A0A449A4P1", "cog_id": "COG0055", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_A0A449A4P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A4P1" ], "product": "F0F1 ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "GPKLONFDEH_149", "locus": "GCA000006625_00109" }, { "type": "cds", "sequence": "contig_1", "start": 66507, "stop": 66806, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTGTAAAAAAAATAAATTGAAAATGTTTGGTTTTATTGCTATTGGAACTGTTTTAACTAGTGCAATAATTCCTTTTTTAGTTACATGCGCAAAAGAGAAAAAAATAAAGCAAGAAGTTATAAAACCAAAACAAATAAGTAATTTAAATCTTGAAGCTAAAAATATTGATATTGTTTTAAAAAATAATGTCTTCAATAAAGAAACAGCAAAATTTACATTGGATTTTGACATAAATAAAGAGTTAAATTTACAAATGGTTGCTGTTTTTGATGTTTTTGATAAAAAAAATAACTGA", "aa": "MVCKKNKLKMFGFIAIGTVLTSAIIPFLVTCAKEKKIKQEVIKPKQISNLNLEAKNIDIVLKNNVFNKETAKFTLDFDINKELNLQMVAVFDVFDKKNN", "aa_hexdigest": "afa5ef12910c9643e0b230d4714468cf", "start_type": "GTG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11291.379699999987, "isoelectric_point": 9.548879814147949 }, "id": "GPKLONFDEH_150", "locus": "GCA000006625_00110" }, { "type": "cds", "sequence": "contig_1", "start": 66840, "stop": 67526, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAAAAAATTTGTTTTTGACAGTTTAGATGAGAATGTTAAATATCTACTAAAACAAATTGATTTTTACGATCCAAAAGCGCCTAATCAAATTATTTATACAAAAAACAATCAGACATTTAGTTTTGATACTAAAATGGATGATAGCATCGCTAGAACTGTTGAACTAATTATTATTAGTGGGTTTAATGGTTTTGTTGATGAACAAATTGTTCCAGATTATAATAAACAAACTACATATTTAAAAGCACCCGGGTTAATTCGATTATTAAATGAAGTTAATAAAATTAAAAAAGAACGAGGTGCGAATAATGTTGCTGTAGTTATGGGTGGAAATAATTATTATGAAAATAATATATCTTCATTAACTAGAGCTCAATATACAACAGCCGCATTAAAGTTTTTAAATCCAGTAGCTACTAGCATCGCTAATACTGATTTAAATTGGGGTTATGATTTAATTGACGAAAAAACAAAGCATTTTAAAGAATACGTCAAAGCTTTATATGGTAATGATGGTATTTTATCTGTTAACTCTTATCTAAAAGATGGTGATAAAAACATACAAATGACTAATAATTCAAAAATTATTAACTTTGGTGGTTTTAAATTAGGATTAATTGGAGCAATGAGTAATTTAATTAATTTTGAATCTAATCTTAATTTAACAAAAAATATTCAATGA", "aa": "MDKKFVFDSLDENVKYLLKQIDFYDPKAPNQIIYTKNNQTFSFDTKMDDSIARTVELIIISGFNGFVDEQIVPDYNKQTTYLKAPGLIRLLNEVNKIKKERGANNVAVVMGGNNYYENNISSLTRAQYTTAALKFLNPVATSIANTDLNWGYDLIDEKTKHFKEYVKALYGNDGILSVNSYLKDGDKNIQMTNNSKIINFGGFKLGLIGAMSNLINFESNLNLTKNIQ", "aa_hexdigest": "0f99eef11cc9d57733c02a904e50479d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25816.017500000024, "isoelectric_point": 7.730978202819824 }, "id": "GPKLONFDEH_151", "locus": "GCA000006625_00111" }, { "type": "cds", "sequence": "contig_1", "start": 67551, "stop": 68870, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02872.23" ], "nt": "TTGATTAATGATGAGGCCGATAAGCTTAAGGATCAAGGTGTTAAAAATATTGTTTTATTATCAAGTTTTTTAGCAGATAAAGCAAAGGAAAATGACACTTTAACTAATGATGAAAAATCTTTTTATAAAGAAGCATCACGAATGAGTAAATTAATAGATAATAATGTCAATTTAATGAGTGTTATTAATCCTTATAAAGCACTTAATGTAATTGCTAATAATAAAAATAATCAACCTTTGAATGTTGTTCAATCAAAACCTAATAGTGGTTTTGTACGATATATGATCACACTAAATCCTTATAACCGTATTTTAAAAACAGAAATTAAGCTTGTTGATAAACTGTCAACAAGCGGTGATAATGCAGGATATCATTATATAACTCAACAAGATTTATTAAATCCACAAAAAAATCCTGATTTCAATCAAAATTTGATTACATTAAATAATTTTCTTAATAATTATAAAGATCATGAATACGAGCAATTAAGAGAAAAAATACTGATTAAAAATGATAAAAAAGTCAATTTATATTCTTCAACATATTCACTTAGTTCCTTAGGAGCGTTTATAAGTGATATATTAGCAAAATACTATCAAGCTCCTTTTTATAATAAACAAACACATACTTTTGAATTTAAACAAACACAAGCTATCCTATTAGCTCCTCATGCTATTCGTTCTTTGGTTTCTGCTAAACCTATTAAACTAGAAAATTTAAATCAAATTTTACCTTTTAATGATACGTTAGTATCAGGTGAAATTAGTGTTAAAAATCTTAAAGAATTTTTAATTAACAAGAATAAATCTCAAAATTACAACGAACCATATCAATTTAGTAATACGCTAAAAATTGAATATGAAAACTTACAAGAAACAAAAACAAAGAAAAAATCAAGAAAAAAACAATCAGAACAAAGTTATAGTGATAATGAAAAAAAAGATGGTCTAATTTTAAGTCATCAAAAAAGAGTTATTAAAGTCTTTTTGAAAAATTCAGATGGTACGTATACAGAATTAAATGATAATCAAAAAATTATAGTAAGTGTTATGAATTCGATCTTAAAATTTAATAAAAAATTTAGTGAGGCTCTTTTAAATAAAACATACGCTGACCAAACTAATATTCGTGATTTGCTAAGAACATACTTACCAAAAGAAACACAAATCGATCAAAAATATGATCAAAGTGTGTTTAGTAAAAATGATGCTCGTTTTTATAAAAAAAAAGAAACAGACCAAAATAAATCACTTATTTACAATCATATTAATGAAAGTATTAATAATAAACAAGCCGATCATTTAAGAATTGGGCACTGA", "aa": "MINDEADKLKDQGVKNIVLLSSFLADKAKENDTLTNDEKSFYKEASRMSKLIDNNVNLMSVINPYKALNVIANNKNNQPLNVVQSKPNSGFVRYMITLNPYNRILKTEIKLVDKLSTSGDNAGYHYITQQDLLNPQKNPDFNQNLITLNNFLNNYKDHEYEQLREKILIKNDKKVNLYSSTYSLSSLGAFISDILAKYYQAPFYNKQTHTFEFKQTQAILLAPHAIRSLVSAKPIKLENLNQILPFNDTLVSGEISVKNLKEFLINKNKSQNYNEPYQFSNTLKIEYENLQETKTKKKSRKKQSEQSYSDNEKKDGLILSHQKRVIKVFLKNSDGTYTELNDNQKIIVSVMNSILKFNKKFSEALLNKTYADQTNIRDLLRTYLPKETQIDQKYDQSVFSKNDARFYKKKETDQNKSLIYNHINESINNKQADHLRIGH", "aa_hexdigest": "e45334296dfc53263e94e17b0d2c68a2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02872.23", "name": "5_nucleotid_C", "length": 151, "aa_cov": 0.33940774487471526, "hmm_cov": 0.7086092715231788, "evalue": 1.605461912157274e-08, "score": 30.402069091796875, "start": 180, "stop": 328 } ], "seq_stats": { "molecular_weight": 50960.132199999985, "isoelectric_point": 9.38300266265869 }, "id": "GPKLONFDEH_152", "locus": "GCA000006625_00112" }, { "type": "cds", "sequence": "contig_1", "start": 69012, "stop": 69593, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGGAATCATTAAGTATTTAAATCAGTTAAGTGGCTCAGATGATTATGATTTTTTAATTAGTGATAATCTTAAAGGTAGTGAAAATGATAAATTAGCAACAAACGAACATACAAGTACTGAACGAATTGGGATAATTTATAATAAGAAAAAAGTTAAACCTATTCCGTTTGCAAATAATAAAATCGGTTATATTTATTCAAATCCATTACAACCTGGCAAATATGAAAAAGCAGAAACTGATTATTCACGTCCACCATTTGCTATTAAATTTGCTTCAATTGGTCAAATTAAAAATGATTTTACATTGGTTTTCGGTCATCTTGATAGTCCTGGAACTTATCCATCAAATACAGAATATCAAAAGAAAGAGCATCTACTAAAACGTACAGAATTAGTTAATAAAAAAGTAATTTCAACGAACCAAATGATTAATAAATATGAACAAGGAAGTCGTGAAGTGGACGATGCTGAACGATTAGTCAAAGTTATGGAAGAAATTAAAAAAATTGATAACAATAAGGATGATGATTATTTCTTTTTAGGTGATACTAATATTCGTTTACATAATCAACAATGA", "aa": "MIGIIKYLNQLSGSDDYDFLISDNLKGSENDKLATNEHTSTERIGIIYNKKKVKPIPFANNKIGYIYSNPLQPGKYEKAETDYSRPPFAIKFASIGQIKNDFTLVFGHLDSPGTYPSNTEYQKKEHLLKRTELVNKKVISTNQMINKYEQGSREVDDAERLVKVMEEIKKIDNNKDDDYFFLGDTNIRLHNQQ", "aa_hexdigest": "1b2e1432c4797d888874e3c48cbc0b6d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22260.81180000001, "isoelectric_point": 6.916930198669434 }, "id": "GPKLONFDEH_153", "locus": "GCA000006625_00113" }, { "type": "cds", "sequence": "contig_1", "start": 69807, "stop": 69923, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTAGTTTATACCCTAATGAATATCATGATAAATTATTTTATATTCGTAACGATATTTCTGATCATGCACCTACATTCTTTGATTTAGGTTTAAATAAAAACGACACTAAATAA", "aa": "MRSLYPNEYHDKLFYIRNDISDHAPTFFDLGLNKNDTK", "aa_hexdigest": "73625463e38a040807382eb00e526749", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4590.048200000001, "isoelectric_point": 5.981758308410644 }, "id": "GPKLONFDEH_154", "locus": "GCA000006625_00114" }, { "type": "cds", "sequence": "contig_1", "start": 70018, "stop": 70164, "strand": "+", "frame": 1, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P58061", "UniRef:UniRef90_Q9PR89" ], "nt": "ATGGCATTAACAATTGTTTTAATCTTGTTTAGTGTTCTAGCTTTAATAATAGGTTTATTATTATCAAGAACTTCACCATCTGGTGGATTGTCAAGTTTAAATGGACAAGATCTAGAAATTTTTAAAAAAACAAAAGACCGTGGTTGA", "aa": "MALTIVLILFSVLALIIGLLLSRTSPSGGLSSLNGQDLEIFKKTKDRG", "aa_hexdigest": "71e74320b93577c3a7cc384bef79344a", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "secG" ], "truncated": "3-prime", "pseudogene": { "start": 70018, "stop": 70242, "observations": { "mutation": [ 70162 ], "stop": [ 70162 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR89", "query_cov": 1.0, "subject_cov": 0.6486486486486487, "identity": 1.0, "score": 85.5, "evalue": 5.97e-21, "gene_start": 1, "gene_end": 48, "reference_start": 1, "reference_end": 48, "reference_sequence": "MALTIVLILFSVLALIIGLLLSRTSPSGGLSSLNGQDLEIFKKTKDRGWIKGLQVFMFLLTIVMILIIIFYRVS" }, "paralog": false, "identity": 0.9864864864864865, "score": 135.0, "evalue": 3.36e-43, "description": "Internal stop codon at 70162. Nonsense mutation around 70162.", "psc": { "uniref90_id": "UniRef90_Q9PR89", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_P58061", "go_ids": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR89", "UniRef:UniRef50_P58061", "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P58061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P58061" ], "product": "Probable protein-export membrane protein SecG" } }, "id": "GPKLONFDEH_155", "locus": "GCA000006625_00115" }, { "type": "cds", "sequence": "contig_1", "start": 70243, "stop": 71310, "strand": "+", "frame": 1, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_Q9PR88", "UniRef:UniRef90_Q9PR88" ], "nt": "ATGAGTGATTTTACGAAACAAACTATTACCGAAGTAATTTCTAAGGAAGAGCGCCCAATACCCGCTGCTATTCTTGCTAAAAAAGTGTTAGAAAAAATACCCACTTTAAATAAAACTGATGTTTATAAACTAATTGATTTATTAATTCAAGAAAATACTATTAAAAAATTAGAAAATAATCGATTGGTTATTGGTTATCTAGATTATGAATTTGATCACGAGATCAAACAAGGAATAATTACTATCAATTCAAAAGGTGATGGCTTCATTAAAGAAGATAATACTGAAATTGAATATTACGTCAACAAAAAATATTTAAATGGGGCTTTAAAAAAAGATTCAGTTAAATTTGTAAAGTTAAAAAAAGAACCTAAAAACAACCTGCAAGATGCTGCTGTAATTGAAATTGTTGGGCATGCTAAAGACCATTATGTTGGTCAATTTATAACTCTTCCTAATGGTGGTTATTACATTTTTGTTGACGATCCTTTATTTTACTTAAATATAAATTTAAAAGATACTACCGGTTTAGTTAATGGTCACAAAATTCTTTTTAAAATTATTTCGCAAACAACAAAAGATGCTATTGCAGAATTAGTTCATATCATAGGTCATAAAAACGATGTTGGTTCTGATGTTTTATCAATCGTTTATGATAATGGTATTGATCCAACTTTTGATCCACAAGTTGTTGATTTGGCTTCAAAATTAGAATTTTATGTTGATGAACATCAAAACAAAATTCGACGTTCAATAATTGATCGTGAAATTATTTCTATTGATCCTGTTGGTTCTAAAGATATTGATGATGCTGTTTATGTTAAAAAATTAAATGATCAACGTTACTTTTTAGGTATTTCTATTGCTGATGTTAGTTTTTATGTACAACCTAACACCATTCTTGATGCTGATGCTTTTAAAAGGGGAACATCAACATATTTAGTTGATCGTGTTATTCCAATGCTTCCGCATAATATTTCTAATAATATTTGTTCATTAAACGAAGGTGAGTTTCGCATGTGTATAACATGTGATATGGTAATTGACAAAGATGGAAAAATATGTTGA", "aa": "MSDFTKQTITEVISKEERPIPAAILAKKVLEKIPTLNKTDVYKLIDLLIQENTIKKLENNRLVIGYLDYEFDHEIKQGIITINSKGDGFIKEDNTEIEYYVNKKYLNGALKKDSVKFVKLKKEPKNNLQDAAVIEIVGHAKDHYVGQFITLPNGGYYIFVDDPLFYLNINLKDTTGLVNGHKILFKIISQTTKDAIAELVHIIGHKNDVGSDVLSIVYDNGIDPTFDPQVVDLASKLEFYVDEHQNKIRRSIIDREIISIDPVGSKDIDDAVYVKKLNDQRYFLGISIADVSFYVQPNTILDADAFKRGTSTYLVDRVIPMLPHNISNNICSLNEGEFRMCITCDMVIDKDGKIC", "aa_hexdigest": "bae008414f5969d98d1a9b341a1fa5ec", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rnr" ], "truncated": "3-prime", "pseudogene": { "start": 70243, "stop": 71613, "observations": { "mutation": [ 71308 ], "stop": [ 71308 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR88", "query_cov": 1.0, "subject_cov": 0.492371705963939, "identity": 1.0, "score": 701.0, "evalue": 1.91e-249, "gene_start": 1, "gene_end": 355, "reference_start": 1, "reference_end": 355, "reference_sequence": "MSDFTKQTITEVISKEERPIPAAILAKKVLEKIPTLNKTDVYKLIDLLIQENTIKKLENNRLVIGYLDYEFDHEIKQGIITINSKGDGFIKEDNTEIEYYVNKKYLNGALKKDSVKFVKLKKEPKNNLQDAAVIEIVGHAKDHYVGQFITLPNGGYYIFVDDPLFYLNINLKDTTGLVNGHKILFKIISQTTKDAIAELVHIIGHKNDVGSDVLSIVYDNGIDPTFDPQVVDLASKLEFYVDEHQNKIRRSIIDREIISIDPVGSKDIDDAVYVKKLNDQRYFLGISIADVSFYVQPNTILDADAFKRGTSTYLVDRVIPMLPHNISNNICSLNEGEFRMCITCDMVIDKDGKICWKDVYPAIMKNYRQMSYDEVNDFYEGKSRFESATLTMKEMLLEAKELHHILRNKKIKDGYVDFDIKEPKIILDETGVPIDIKIYERKTAQMMVEDFMIAANEAVTMFAEEHMDKTLKEFNLEMPFIYRVHDKPSIINLQKFEIEAKKLSFNISHDFENIQPNTISNWLKMNDNHVNLPLISKLLLRSMAKASYEIINTGHFGLASDNYTHFTSPIRRYPDLIVHRLLWMFIFDSQSYTDKQRVELVNKLKLITEESNKNEIIAVKTERDVNAAKFAEYMNLHIGKEFIGVVTTVSSFGVFVELENTIEGLIRIKNLKDDFYDFIPENMTLVGQKRKKIITVGNKVRVRVIEANKLTRKIDFELVAQ" }, "paralog": false, "identity": 0.9978070175438597, "score": 906.0, "evalue": 0.0, "description": "Internal stop codon at 71308. Nonsense mutation around 71308.", "psc": { "uniref90_id": "UniRef90_Q9PR88", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_Q9PR88", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR88", "EC:3.1.13.1", "UniRef:UniRef50_Q9PR88", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR88" ], "product": "Ribonuclease R" } }, "id": "GPKLONFDEH_156", "locus": "GCA000006625_00116" }, { "type": "cds", "sequence": "contig_1", "start": 71332, "stop": 71808, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24" ], "nt": "ATGAAAAATTATCGTCAAATGTCATATGATGAAGTTAATGATTTTTATGAAGGAAAATCACGTTTTGAATCAGCAACATTAACTATGAAAGAAATGTTATTAGAAGCTAAAGAATTGCACCATATTTTAAGAAATAAAAAAATTAAAGATGGTTATGTTGATTTTGATATTAAAGAACCAAAAATTATTTTAGATGAAACTGGTGTCCCTATTGATATTAAAATTTACGAACGTAAAACTGCTCAAATGATGGTTGAAGATTTTATGATTGCAGCTAATGAGGCTGTAACTATGTTTGCTGAAGAACACATGGATAAAACACTAAAAGAATTTAATTTAGAAATGCCTTTTATCTATCGTGTTCATGATAAACCATCAATTATTAACTTACAAAAATTTGAAATTGAGGCAAAAAAATTGAGTTTCAATATCTCACATGATTTTGAGAACATTCAACCTAACACTATTTCAAATTGA", "aa": "MKNYRQMSYDEVNDFYEGKSRFESATLTMKEMLLEAKELHHILRNKKIKDGYVDFDIKEPKIILDETGVPIDIKIYERKTAQMMVEDFMIAANEAVTMFAEEHMDKTLKEFNLEMPFIYRVHDKPSIINLQKFEIEAKKLSFNISHDFENIQPNTISN", "aa_hexdigest": "77ac5994b74e8bdc545ce37bb204e193", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 161, "aa_cov": 0.9683544303797469, "hmm_cov": 0.9316770186335404, "evalue": 3.840682392275539e-30, "score": 100.7998275756836, "start": 1, "stop": 153 } ], "seq_stats": { "molecular_weight": 18749.36790000001, "isoelectric_point": 5.292926597595215 }, "id": "GPKLONFDEH_157", "locus": "GCA000006625_00117" }, { "type": "cds", "sequence": "contig_1", "start": 71815, "stop": 71991, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24" ], "nt": "ATGAATGATAATCATGTTAACTTACCATTAATTAGTAAATTATTATTAAGATCAATGGCTAAAGCTTCTTATGAAATTATTAATACGGGACATTTTGGTTTAGCATCTGATAACTATACACACTTTACATCACCAATACGGCGTTATCCAGATTTAATTGTACACCGTTTATTATGA", "aa": "MNDNHVNLPLISKLLLRSMAKASYEIINTGHFGLASDNYTHFTSPIRRYPDLIVHRLL", "aa_hexdigest": "6971f96e19a9ce6d74715c3be36ab6b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 55, "aa_cov": 0.9482758620689655, "hmm_cov": 1.0, "evalue": 1.6562468312512445e-24, "score": 82.2816162109375, "start": 4, "stop": 58 } ], "seq_stats": { "molecular_weight": 6678.656799999999, "isoelectric_point": 9.395573997497557 }, "id": "GPKLONFDEH_158", "locus": "GCA000006625_00118" }, { "type": "cds", "sequence": "contig_1", "start": 71992, "stop": 72408, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00575.29" ], "nt": "ATGTTTATTTTTGATTCTCAAAGTTATACAGATAAACAACGAGTAGAATTAGTAAATAAACTGAAGTTAATTACTGAAGAATCAAATAAAAATGAAATTATAGCAGTTAAGACTGAACGTGATGTTAATGCTGCTAAATTTGCTGAATATATGAATTTACATATTGGTAAAGAATTTATTGGTGTGGTAACAACAGTTTCTAGTTTTGGTGTTTTTGTTGAACTCGAAAATACGATTGAAGGTTTAATTAGAATTAAAAATTTAAAAGATGATTTTTATGATTTTATTCCTGAAAACATGACTTTAGTTGGTCAAAAAAGAAAGAAAATTATTACTGTTGGCAATAAAGTTCGTGTACGTGTTATTGAAGCTAACAAACTTACAAGAAAAATTGATTTTGAACTAGTTGCACAATAA", "aa": "MFIFDSQSYTDKQRVELVNKLKLITEESNKNEIIAVKTERDVNAAKFAEYMNLHIGKEFIGVVTTVSSFGVFVELENTIEGLIRIKNLKDDFYDFIPENMTLVGQKRKKIITVGNKVRVRVIEANKLTRKIDFELVAQ", "aa_hexdigest": "ce78b5a220f0755ae06e9358bab16193", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00575.29", "name": "S1", "length": 80, "aa_cov": 0.5797101449275363, "hmm_cov": 0.8625, "evalue": 3.906999104185493e-14, "score": 48.44105911254883, "start": 55, "stop": 134 } ], "seq_stats": { "molecular_weight": 15930.30679999999, "isoelectric_point": 8.776226234436034 }, "id": "GPKLONFDEH_159", "locus": "GCA000006625_00119" }, { "type": "cds", "sequence": "contig_1", "start": 72608, "stop": 73033, "strand": "+", "frame": 2, "gene": "smpB", "product": "SsrA-binding protein SmpB", "db_xrefs": [ "COG:COG0691", "COG:O", "GO:0003723", "GO:0005829", "GO:0070929", "KEGG:K03664", "RefSeq:WP_006688578.1", "SO:0001217", "UniParc:UPI0000136037", "UniRef:UniRef100_Q9PR87", "UniRef:UniRef50_Q9PR87", "UniRef:UniRef90_Q9PR87" ], "nt": "ATGATTGTATCAAATAAACACGCACGTCGAAATTATGAATTACTAGATTTTTTTGAATGTGGAATCGTTTTAAAAGGAACTGAAGTTAAATCTATTTCACGTGCTAATTGCTCAATTAATGAAGCTTATGTCCAAATAATTAAAAATCAAGCTTTAATTTTAAATATGCATGTTGCAAATTTTTTTGAGGGTAATAACTTTAATCAAGATCCATACCGGAATCGAAAACTATTATTACACAAAAAAGAAATTATTAGACTTCAACATTTAGTGAAAACACAACATATGACAATTGTGCCAACCAAAATTTATTGGAAAAATAATAAACTAAAGATAGAGATTGCTTTAGGAAAAGGTAAACAGCTTCATGATAAACGTGAGGATATAAAAAAAAGAGATTTAGCGCGTGAAACGCGTTTATTTTAA", "aa": "MIVSNKHARRNYELLDFFECGIVLKGTEVKSISRANCSINEAYVQIIKNQALILNMHVANFFEGNNFNQDPYRNRKLLLHKKEIIRLQHLVKTQHMTIVPTKIYWKNNKLKIEIALGKGKQLHDKREDIKKRDLARETRLF", "aa_hexdigest": "86048fe7fb3a657f4dcce40194de455f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000136037", "ncbi_nrp_id": "WP_006688578.1", "uniref100_id": "UniRef100_Q9PR87", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000136037", "RefSeq:WP_006688578.1", "UniRef:UniRef100_Q9PR87" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR87", "uniref90_id": "UniRef90_Q9PR87" }, "psc": { "uniref90_id": "UniRef90_Q9PR87", "gene": "smpB", "product": "SsrA-binding protein SmpB", "uniref50_id": "UniRef50_Q9PR87", "cog_id": "COG0691", "cog_category": "O", "kegg_orthology_id": "K03664", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0070929" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR87" ], "product": "SsrA-binding protein" }, "genes": [ "smpB" ], "id": "GPKLONFDEH_160", "locus": "GCA000006625_00120" }, { "type": "cds", "sequence": "contig_1", "start": 73074, "stop": 73250, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAATAAAACATAAATTTTTATTTTTCTTTATTTTATTTTTAAATTTAGTACAAGTTTTATTAGTAATTGGTTCTCCTCTTTTATTATCAATTCTTAATAATGGTAATTATGCATCAACAACCGCTGACGCTTTTATTTATGGCGGTGTGTTAATCGCCATGGCTATAGGATGA", "aa": "MLIKHKFLFFFILFLNLVQVLLVIGSPLLLSILNNGNYASTTADAFIYGGVLIAMAIG", "aa_hexdigest": "786b25340e8d71559d049fce064e74cd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6330.626700000001, "isoelectric_point": 8.210645103454592 }, "id": "GPKLONFDEH_161", "locus": "GCA000006625_00121" }, { "type": "cds", "sequence": "contig_1", "start": 73341, "stop": 73520, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTAACATATGCAGATAAACAAAAATTTAATCAATCAATTTTATTAACAACTTCAATAAAAGACGTCGAAGAACTTTCAAAAACAATTATCTTTTTAACACGACCTGTAATTATGGGAATTTTTTATTTCATTGGAGCAATTATATTATTATTCATTATTAGTGGTAATTTATGA", "aa": "MFLTYADKQKFNQSILLTTSIKDVEELSKTIIFLTRPVIMGIFYFIGAIILLFIISGNL", "aa_hexdigest": "74790b5724ad9c75d59afe43aaa35c1d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6744.047799999995, "isoelectric_point": 8.09853458404541 }, "id": "GPKLONFDEH_162", "locus": "GCA000006625_00122" }, { "type": "cds", "sequence": "contig_1", "start": 73469, "stop": 73801, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAAAGTAACAATAGCTATGCTTACTTTACTAATTCGATTGGCAATAATAGATTTAGAAACATTATCTTTTCATTTAAAATCTAATTTTGTTAAAAAAAATGCATCTAATAAGAAAGAACGAATAACTTTTACTCCACTAACATTTTCTAATGACACTTTTGCTAATTCATCATTAGCAACACGCATTTTATTTCAATTTTTAGCATTAAATTTTGAAATAATCATAAAAATAATAATTGTTGATAAAGCCATCAAAACTACAGTTAAAGGGATTTGTCATAAATTACCACTAATAATGAATAATAATATAATTGCTCCAATGAAATAA", "aa": "MIKVTIAMLTLLIRLAIIDLETLSFHLKSNFVKKNASNKKERITFTPLTFSNDTFANSSLATRILFQFLALNFEIIIKIIIVDKAIKTTVKGICHKLPLIMNNNIIAPMK", "aa_hexdigest": "42f3aa718d3140b15216177aca694483", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12459.982799999982, "isoelectric_point": 10.149854087829588 }, "id": "GPKLONFDEH_163", "locus": "GCA000006625_00123" }, { "type": "cds", "sequence": "contig_1", "start": 73851, "stop": 74084, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAACAAACATTTTATCAATCATGCAATCAACTAATTTATTAGTTTTAGGAATTGCGTTAATTGGTTTTGGAAGCGAACAGTATTTTAGATCTATAGTAAGTATTAAAAGAATTAATGATTTAATTTTTTATAAAAATCAAATTGTTTATAATGGTACAAAAGAAATTAATAACAATAATATTGAATTCAAAAATGTAAGTTTTAGGTATCATAATTCAAAAAAATGA", "aa": "MKATNILSIMQSTNLLVLGIALIGFGSEQYFRSIVSIKRINDLIFYKNQIVYNGTKEINNNNIEFKNVSFRYHNSKK", "aa_hexdigest": "4a736d690495bbe4eaf80b35ca1ee336", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8917.234199999995, "isoelectric_point": 9.917123222351073 }, "id": "GPKLONFDEH_164", "locus": "GCA000006625_00124" }, { "type": "cds", "sequence": "contig_1", "start": 74181, "stop": 74792, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCATCAATTAGAACCAAATAGCGGAGAAATATTTATTGATAATATTCCTTTAAAAGATATTAAAAAAACAAGTTTGCAAGCTGCTTTTAGCCTTAATGAACAAGTCCCTTTGCTTTTTTATGGAACTATCCGTGATAACGTATTATTTGGTTTAGAGGATATTGATGAAAACAAAATCAACGAAGTTATTGAAATTGCACAAGCTAAGGATTTTATTGAATCTAAGAATGAAAAATTAGAATCTCTAATAGCACAAAGGGCTCAAAATCTATCTGGAGGACAAAAACAACGTCTAGCAATGGCACGTGCATTGTTGCGCGACGCAAGAACTTTAGTGCTAGATGATTCAACTAGCGCATTAGACATGATCACTGAAAAAAAGTTTTTAACTGCACTAAAACAACAAATTAAATTTAAGAATTCGATTATTATTGCTCAAAGAATTAATATTATTTTAGACTGTGACAAAATTATTGTAATGGATAAAGGTAAGATTGTTGGAATTGGTAATAATGAGACATTACTTAAAACTTGTGCAGTTTATAAAAATATCTATCAACATCAAATAAATAAAGGAGCAGAATATGAGCCAACAACTACAAACCAATAA", "aa": "MHQLEPNSGEIFIDNIPLKDIKKTSLQAAFSLNEQVPLLFYGTIRDNVLFGLEDIDENKINEVIEIAQAKDFIESKNEKLESLIAQRAQNLSGGQKQRLAMARALLRDARTLVLDDSTSALDMITEKKFLTALKQQIKFKNSIIIAQRINIILDCDKIIVMDKGKIVGIGNNETLLKTCAVYKNIYQHQINKGAEYEPTTTNQ", "aa_hexdigest": "e3ad02a4d7515da098abbb57190c2fb2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 119, "aa_cov": 0.5812807881773399, "hmm_cov": 0.8823529411764706, "evalue": 1.574204865272327e-15, "score": 53.541831970214844, "start": 2, "stop": 119 } ], "seq_stats": { "molecular_weight": 22920.180500000017, "isoelectric_point": 6.175238227844238 }, "id": "GPKLONFDEH_165", "locus": "GCA000006625_00125" }, { "type": "cds", "sequence": "contig_1", "start": 74767, "stop": 74880, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCAACAACTACAAACCAATAAACCTGTAAGTAAACTTTATTTAATTTATTATTATTTTAAGGATTTTAAAAAAGTATTTATTGGTTTGTCAATTGCCATTATTTTTTGA", "aa": "MSQQLQTNKPVSKLYLIYYYFKDFKKVFIGLSIAIIF", "aa_hexdigest": "2d3e448ff3697656ca7f55c7408d5c80", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4449.298999999999, "isoelectric_point": 9.69941349029541 }, "id": "GPKLONFDEH_166", "locus": "GCA000006625_00126" }, { "type": "cds", "sequence": "contig_1", "start": 74971, "stop": 75495, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTGCAGGATTGATAGGAATTTATTTTTTCTCATTTATATTTTTACTAATTATCAACGAATTTGCCATTCGAATATCTTTTAAGATTGAATCCCATTTATCAATGATGGTGATTAATCGTATTCGTTATTTACCAATGAAGTATTTTGATATTAATAAAAGCGGAGAAATTTTTACTAAAACACTAAGTGATCCAACAAGTGTACAAGATGGAATTGTTAATTTTTATATTGAGTTAAATAAAGCTATATTTGGCGCAATTGGTTTTGGAACTGCTTTATTAATAACAAGTCCTTATATTGCTTTAATTGGAATTGTCATTTATGTTCTAGTAATGCTATTTAATATTTTGATATTTAAAAAATCCCATCAATTAATGCGCATAAAACGGCAAAATTTTGGTGAAATGAACGGATATATTGAAGAAATGATTTATGGGCAAAACGTTGTAGCTAATTTTAATGAACAAGTTTTTTTCATAAAAAAATTAGATAATATGATTAAAAAATTATATAAAAATTGA", "aa": "MCAGLIGIYFFSFIFLLIINEFAIRISFKIESHLSMMVINRIRYLPMKYFDINKSGEIFTKTLSDPTSVQDGIVNFYIELNKAIFGAIGFGTALLITSPYIALIGIVIYVLVMLFNILIFKKSHQLMRIKRQNFGEMNGYIEEMIYGQNVVANFNEQVFFIKKLDNMIKKLYKN", "aa_hexdigest": "64a0ad80698c2573da5aeaf4ab42fef0", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20187.907799999994, "isoelectric_point": 9.38364734649658 }, "id": "GPKLONFDEH_167", "locus": "GCA000006625_00127" }, { "type": "cds", "sequence": "contig_1", "start": 75547, "stop": 75807, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGATTAATGAATGCCGTTTTAATTGTGTCTTATTTATTAATTTCTATTAAAGGAATTCATTTGCCAGGGCTTATAAATAATATTGATCCAACAACGAATATTATTTCTTTTGGTGGTTTAGTTAGTATTTCATTATTTGGTAATTTCTTTTGTGATAATTTTAGTCAATTATCAAATACTATTCCTATTTTTATCGTTGCTAGAACATCACTTGCTAAAATTGATGAAATTGTTAAAACTCCTAATGAGATAAATTGA", "aa": "MRLMNAVLIVSYLLISIKGIHLPGLINNIDPTTNIISFGGLVSISLFGNFFCDNFSQLSNTIPIFIVARTSLAKIDEIVKTPNEIN", "aa_hexdigest": "2c1b263ad88acae696c294dacf58a17d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9436.02049999999, "isoelectric_point": 6.518375205993652 }, "id": "GPKLONFDEH_168", "locus": "GCA000006625_00128" }, { "type": "cds", "sequence": "contig_1", "start": 75847, "stop": 76563, "strand": "+", "frame": 1, "gene": null, "product": "Mitochondrial potassium channel ATP-binding subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6J7TFN9" ], "nt": "GTGCGTTTTGAAAATGTTAATTTTAATTATTTAAAAAATACACCTACCTTAAAAAATATTAATTTTGTTGCCAAAAGGAACCAAAGAATTGCTATTATTGGACCCACAGGTGCTGGAAAAACAACAATTACTAACTTAATTAATAAATTTTATGATATTAATAATGGCTTTATTTATTTTAACGATGTTGACATTACAAATAAATCCCGTGAAAGTGTACGTGAACATATTTCTATTGTTTTACAAGATCCTTTTTTATTTAGTGAAAGTATTTATGAGAATATCAAAAAAGGTAAAATGAATGCAACAAAAGAAGAAATTATTGCAGCAGCTAAAAAAGCTCAAGTTCATGATTTGATCCTTTCATTTCCAAAAGATTATGCTACAGTTATTGATGAAAAACAAAACTTATCACAAGGTCAAAAACAATTAATAACTATCGCTCGTGCTATTATTTCTGATGCGAAAATTATTATTTTAGACGAAGCAACAAGCTCTGTGGATACGCAAACCGAACATAAATTACAATTAGCAATTAATAATTTACTAAAAGGACGAACATCATTTATTATTGCCCATCGATTATCAACAATTATTAACTCTGATTTAATTCTTGTAATTAAAGATGGTGAAATTATTGCACAAGGGAATCATGATTATTTGATTAAGAATTCGCCTTTTTATCAAGAACTTTACTATGCAAATTTTAATGAATAA", "aa": "MRFENVNFNYLKNTPTLKNINFVAKRNQRIAIIGPTGAGKTTITNLINKFYDINNGFIYFNDVDITNKSRESVREHISIVLQDPFLFSESIYENIKKGKMNATKEEIIAAAKKAQVHDLILSFPKDYATVIDEKQNLSQGQKQLITIARAIISDAKIIILDEATSSVDTQTEHKLQLAINNLLKGRTSFIIAHRLSTIINSDLILVIKDGEIIAQGNHDYLIKNSPFYQELYYANFNE", "aa_hexdigest": "54d3983cd01744b8ca1495e721b2d120", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003217A8C9", "query_cov": 0.9873949579831933, "subject_cov": 0.8545454545454545, "identity": 0.528, "score": 240.0, "evalue": 3.86e-76, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A6J7TFN9" }, "pscc": { "uniref50_id": "UniRef50_A0A6J7TFN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6J7TFN9" ], "product": "Mitochondrial potassium channel ATP-binding subunit" }, "genes": [], "id": "GPKLONFDEH_169", "locus": "GCA000006625_00129" }, { "type": "cds", "sequence": "contig_1", "start": 76623, "stop": 76955, "strand": "+", "frame": 3, "gene": null, "product": "putative protein UU061", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_Q9PR84", "UniRef:UniRef90_Q9PR84" ], "nt": "ATGACATCGTTAGACATAACCACGTTAGTAATTAGTATACTTTCGTTAATTGCAACACTATCAATTTCTTTTAATATTTATTTTATTGAATTAAGAAAACAACGCGAACGTAAAATTGAACGTCTACAACAAGAAGCAAAACAATTTATTATTAATAACTTAGATGAAAAAGATTTTATTGTTTTGTGTCAATTTATTTATAAATTATATAAACATGATAAACATACAAGAAAGATTCATTATGAATTTGTTTTATTAAATGAACCTGTTCAAAAAGAGGTTTTTAAACAACTAGAAATTCTAAATATAGTTGATTTTAAAGATAATGAATGA", "aa": "MTSLDITTLVISILSLIATLSISFNIYFIELRKQRERKIERLQQEAKQFIINNLDEKDFIVLCQFIYKLYKHDKHTRKIHYEFVLLNEPVQKEVFKQLEILNIVDFKDNE", "aa_hexdigest": "76432c381de7f579740f264bf760abe9", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 76623, "stop": 77258, "observations": { "mutation": [ 76953, 77013 ], "stop": [ 76953, 77013 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR84", "query_cov": 1.0, "subject_cov": 0.4166666666666667, "identity": 1.0, "score": 204.0, "evalue": 2.35e-64, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MTSLDITTLVISILSLIATLSISFNIYFIELRKQRERKIERLQQEAKQFIINNLDEKDFIVLCQFIYKLYKHDKHTRKIHYEFVLLNEPVQKEVFKQLEILNIVDFKDNEWIANKFSILKEIVNDYQLGNWDDYKFYENFNFAYTLFREEKCDAVFEEIKQNKFGGKNLSFHEYLNEYAHRSKESDMLAPIDYFIDQNNLNNVFDRQKHAIYKLYLLDLILNELCRSMNNDHRINNEFKYFDCEVIYVEDLYLKILYKLYFQLN" }, "paralog": false, "identity": 0.990521327014218, "score": 417.0, "evalue": 6.89e-151, "description": "Internal stop codon at 76953, 77013. Nonsense mutation around 76953, 77013.", "psc": { "uniref90_id": "UniRef90_Q9PR84", "product": "Uncharacterized protein UU061", "uniref50_id": "UniRef50_Q9PR84", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR84", "UniRef:UniRef50_Q9PR84", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR84" ], "product": "Uncharacterized protein UU061" } }, "id": "GPKLONFDEH_170", "locus": "GCA000006625_00130" }, { "type": "cds", "sequence": "contig_1", "start": 77181, "stop": 77417, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCTCCAATCGATTATTTTATTGATCAAAATAATTTAAATAATGTTTTTGATCGTCAAAAACACGCTATTTATAAACTTTATTTGCTTGATTTGATTTTAAATGAATTGTGCCGTTCAATGAATAATGACCATCGTATTAATAATGAATTTAAGTATTTTGATTGCGAAGTTATTTATGTTGAAGATTTATATTTAAAAATTTTATATAAACTTTATTTTCAATTAAATTAA", "aa": "MLAPIDYFIDQNNLNNVFDRQKHAIYKLYLLDLILNELCRSMNNDHRINNEFKYFDCEVIYVEDLYLKILYKLYFQLN", "aa_hexdigest": "56007707b98f68c94b9cf89675339412", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9646.019599999992, "isoelectric_point": 5.127752494812011 }, "id": "GPKLONFDEH_171", "locus": "GCA000006625_00131" }, { "type": "cds", "sequence": "contig_1", "start": 77527, "stop": 78354, "strand": "+", "frame": 1, "gene": null, "product": "Lysyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A401FP47" ], "nt": "ATGGAAAGAAAATTTAGTGATCAAGAATTAATCCGTCGTACACACTTACAAGAATTAAAAAACCAAAATAAAAATCCTTTTTTAATAACAAAAGTTGATAGAAGTATGTTTTTAAAAGACTTTGCTGAAAAATATAAAAATTTTAGCAAAGAAGAATTACATAATATGGATTTAGAGAAATTGACACTTGCTGGTCGTTTAATCGGAATACGTCAAACCTTTGGGATTATTCAAGATTTTTCAGCAAAGTTACAAATTTATATTAATAAAAAAAATGTTGCTCCAGAGGTTTTTTCAACTTTTAAATCATTAGATATTGGTGATATTATTGAATTAGAAGGTGTAGCAATGAAAACTAATAGTGATGAAATTACTTTAAATGTTACAAACATTCGATTAGTTGCTAAAAGTCTAAAAGTTCTCCCTGAAAAGTATCATGGATTGGTTGATGAAGAAATTAAAGCACGTCAACGTTATTTAGATTTAATTGTTAACGATGAGACAAAAAATACTTTTATAAAACGATCTTTAATTATACGTGAAATGCGTAATTGGTTAGATAATAAAGGTTTTTTTGAAGTTGAAACGCCTGTTTTGCAAGATATTTTAAGTGGTGCTGCTGCTCGTCCTTTTATTACACATCATAACACTTTAAATAAAGAATATTATTTAAGAATTGCTACTGAAATTGCTTTAAAAAAGTGTATTATTGGGGGTTTTGAAAAAGTATATGAAATAGGTAGAATTTTTCGAAATGAGGGAATGGATAGTACTCATAATCCTGAATTTACTTCAGTTGAATTATATATTGCATATGTTGATTTATGA", "aa": "MERKFSDQELIRRTHLQELKNQNKNPFLITKVDRSMFLKDFAEKYKNFSKEELHNMDLEKLTLAGRLIGIRQTFGIIQDFSAKLQIYINKKNVAPEVFSTFKSLDIGDIIELEGVAMKTNSDEITLNVTNIRLVAKSLKVLPEKYHGLVDEEIKARQRYLDLIVNDETKNTFIKRSLIIREMRNWLDNKGFFEVETPVLQDILSGAAARPFITHHNTLNKEYYLRIATEIALKKCIIGGFEKVYEIGRIFRNEGMDSTHNPEFTSVELYIAYVDL", "aa_hexdigest": "2ddb4906a6cf4d4057c804f8402b3f39", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0029082C91", "query_cov": 0.9781818181818182, "subject_cov": 0.9962962962962963, "identity": 0.513, "score": 245.0, "evalue": 1.35e-77, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A401FP47", "kegg_orthology_id": "K04567" }, "pscc": { "uniref50_id": "UniRef50_A0A401FP47", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A401FP47" ], "product": "Lysyl-tRNA synthetase" }, "genes": [], "id": "GPKLONFDEH_172", "locus": "GCA000006625_00132" }, { "type": "cds", "sequence": "contig_1", "start": 78361, "stop": 79008, "strand": "+", "frame": 1, "gene": null, "product": "lysine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3M1L5W2" ], "nt": "ATGCAACTAACTGAAGATTTAATTCGTCATATTGCTACTAAATTAAACTTATTAAATCCTACATTTAGAGGTTTTAGTATTGATTTAAATAAACCTTTTAAAAAAGCTCATATGGTTGATTTAATTAATGAATATGTTGGTGTTAATTTTTTTGAAGTTAAAAGCGATGAACAAGCAATTGAATTAGCAAAAAAACACCATGTTAAATTATTAGATCATCAAAAAAACTTTGGTCATATCGTTAATGCTTTTTTTGAAACTTTTGTTGAAGAAAAATTAGTTGAACCAACATTTGTATATGGTCATCCGTTACAAGTTTCTCCACTTACTAAAAAGAATCAGAGTGATCCTCGTTTTGTAGATCGTTTTGAATTATTTATTTGTCAAAAAGAATTTGCTAATGCTTATAGTGAAATTAATGATCCAATTGATCAATATGAACGTTTTATCGCACAGTTAGAAGAAGCTAAATTAGGAAATGACGAAGCAAGTGAATTAGATATGGAATTTATTGAGGCCTTAGAATATGGTATGCCTCCAACAGGTGGTTTGGGAATCGGTGTTGATCGTTTAGTAATGCTGTTAACAAGTAATGATAGTATTCGTAATGTTTTATTATTTCCTCACATGAAAGATAAAACAAAATAA", "aa": "MQLTEDLIRHIATKLNLLNPTFRGFSIDLNKPFKKAHMVDLINEYVGVNFFEVKSDEQAIELAKKHHVKLLDHQKNFGHIVNAFFETFVEEKLVEPTFVYGHPLQVSPLTKKNQSDPRFVDRFELFICQKEFANAYSEINDPIDQYERFIAQLEEAKLGNDEASELDMEFIEALEYGMPPTGGLGIGVDRLVMLLTSNDSIRNVLLFPHMKDKTK", "aa_hexdigest": "d9d91c773349180c4b753b797e2196a9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002E2B0F01", "query_cov": 0.9953488372093023, "subject_cov": 0.8014981273408239, "identity": 0.575, "score": 270.0, "evalue": 1.87e-88, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A3M1L5W2", "kegg_orthology_id": "K04567" }, "pscc": { "uniref50_id": "UniRef50_A0A3M1L5W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3M1L5W2" ], "product": "lysine--tRNA ligase" }, "genes": [], "id": "GPKLONFDEH_173", "locus": "GCA000006625_00133" }, { "type": "cds", "sequence": "contig_1", "start": 79422, "stop": 79772, "strand": "-", "frame": 2, "gene": "rCL", "product": "putative protein UU063", "db_xrefs": [ "COG:COG3613", "COG:F", "SO:0001217", "UniRef:UniRef50_Q9PR82", "UniRef:UniRef90_Q9PR82" ], "nt": "ATGAGTAAAAAAATCAAAATTTATTTAGCTGGTCCTTTATTCACCTTAGCTGAAATTAATGATCGAAAACAACAAGCTAGTTTAATTAGAAAAACATTTAAAGATGAATTACCAAATTATGAACTTGATCTTTTTAATCCGATCGAAGTTAACGACGAATTAGGTGCTAATGCACATAAACCAAATATCTTTTTTTATGAATCAGATATTAAATTTATTGATCAAACTGATATCGCAATTATAGATATTGATAACACTGATGATGGCACCATGGCTGAAATGGGTTATTTTGTAGCACTTCAAAAACACGTTAAACCAACGTTAAAAATTTATATTTTAAATACTGATTGA", "aa": "MSKKIKIYLAGPLFTLAEINDRKQQASLIRKTFKDELPNYELDLFNPIEVNDELGANAHKPNIFFYESDIKFIDQTDIAIIDIDNTDDGTMAEMGYFVALQKHVKPTLKIYILNTD", "aa_hexdigest": "58832fd25ca5817917e7926d9c4ea843", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rCL" ], "truncated": "5-prime", "pseudogene": { "start": 79296, "stop": 79772, "observations": { "mutation": [ 79424 ], "stop": [ 79424 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR82", "query_cov": 1.0, "subject_cov": 0.7341772151898734, "identity": 1.0, "score": 233.0, "evalue": 5.5e-77, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MSKKIKIYLAGPLFTLAEINDRKQQASLIRKTFKDELPNYELDLFNPIEVNDELGANAHKPNIFFYESDIKFIDQTDIAIIDIDNTDDGTMAEMGYFVALQKHVKPTLKIYILNTDWRVHKHRNEVLNKFLDGMILSHCQYFTNFTDLLNHLLIELKK" }, "paralog": false, "identity": 0.9936708860759493, "score": 318.0, "evalue": 5.08e-113, "description": "Internal stop codon at 79424. Nonsense mutation around 79424.", "psc": { "uniref90_id": "UniRef90_Q9PR82", "gene": "RCL", "product": "Uncharacterized protein UU063", "uniref50_id": "UniRef50_Q9PR82", "cog_id": "COG3613", "cog_category": "F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR82", "UniRef:UniRef50_Q9PR82", "COG:COG3613", "COG:F" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR82" ], "product": "Uncharacterized protein UU063" } }, "id": "GPKLONFDEH_174", "locus": "GCA000006625_00134" }, { "type": "cds", "sequence": "contig_1", "start": 79940, "stop": 80206, "strand": "-", "frame": 3, "gene": null, "product": "putative protein UU064", "db_xrefs": [ "GO:0016020", "RefSeq:WP_006688619.1", "SO:0001217", "UniParc:UPI00001391D6", "UniRef:UniRef100_Q9PR81", "UniRef:UniRef50_Q9PR81", "UniRef:UniRef90_Q9PR81" ], "nt": "ATGAGTGACGAACAAAAAACTAAATTAATAGAATTGAAGCAAAAAGTTAATGATCTTGAGTTTGAATTAGATAAAATTAAAAATGAATTTATGATGAAAATAATCATAGCCGTTATTTTAATCTTTTTTTTAACCATAGTAGGGCTATTTTATTTAATAATAAATTTATCAAGAATTATTAGTTTAAATCAAAAAAGAAAATCATTGGAATTAAAAACCAAAGAATTAAACCACGAAATTAATAAAATAGAAATTTCATTATTGTAA", "aa": "MSDEQKTKLIELKQKVNDLEFELDKIKNEFMMKIIIAVILIFFLTIVGLFYLIINLSRIISLNQKRKSLELKTKELNHEINKIEISLL", "aa_hexdigest": "0036e3d3e82694042a30d12ee7546cec", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00001391D6", "ncbi_nrp_id": "WP_006688619.1", "uniref100_id": "UniRef100_Q9PR81", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391D6", "RefSeq:WP_006688619.1", "UniRef:UniRef100_Q9PR81" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR81", "uniref90_id": "UniRef90_Q9PR81" }, "psc": { "uniref90_id": "UniRef90_Q9PR81", "product": "Uncharacterized protein UU064", "uniref50_id": "UniRef50_Q9PR81", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR81" ], "product": "Uncharacterized protein UU064" }, "genes": [], "id": "GPKLONFDEH_175", "locus": "GCA000006625_00135" }, { "type": "cds", "sequence": "contig_1", "start": 80318, "stop": 80620, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATATCAAAAACTAGAACTTGAATATACTAACAAACCAATTGTAGAGGATCTAAATTCAATTTATTCATTATCAAGCATTACTCCACTTCGTATTCCTCATTTTTATGTAGGTAATTTTTATGTATATAAATATGCAGTAGGTCAAGTTGCAGCAATTATTTCAGGCCATCGTGTTTTCACTAAAGTAACAGGTGCAAAACAAAAAGTGTTTGATTTCTTATCAAGTGGTGGTTCAAAAGATCCATTAGATACAATTAAATTACTAGGTGTTGATTTAACAAAACCACAAGCATGA", "aa": "MKEYQKLELEYTNKPIVEDLNSIYSLSSITPLRIPHFYVGNFYVYKYAVGQVAAIISGHRVFTKVTGAKQKVFDFLSSGGSKDPLDTIKLLGVDLTKPQA", "aa_hexdigest": "75145978bccff77ab21c311f7f44c494", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11201.807899999989, "isoelectric_point": 9.126482963562015 }, "id": "GPKLONFDEH_176", "locus": "GCA000006625_00136" }, { "type": "cds", "sequence": "contig_1", "start": 80672, "stop": 80950, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTATACTAACTCATTACGTGATGTACAAACTATTGTTCATGAATTAGGCCACTCAATGCATTCGTTATATTCTAATCGCACACAAAAAATTTATAGTGATTACAAGATTTTCTATGCGGAAATTGCATCAATTTCAAATGAAGTTTATTTAAACTACTATTTATTAGAAAAATACAAGAACGATCTTGAAATGAAATTAATGATTTTAGATGAAATGATTAGTGGGTTCTTCGCAACAACAACAAGACAAGTAATTTTTTCAAATTTTGAATGA", "aa": "MNYTNSLRDVQTIVHELGHSMHSLYSNRTQKIYSDYKIFYAEIASISNEVYLNYYLLEKYKNDLEMKLMILDEMISGFFATTTRQVIFSNFE", "aa_hexdigest": "651f52f2e3b94c70759e559b1aa14ad4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10984.371599999991, "isoelectric_point": 5.526534843444824 }, "id": "GPKLONFDEH_177", "locus": "GCA000006625_00137" }, { "type": "cds", "sequence": "contig_1", "start": 81170, "stop": 81709, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGATTCTCAATTAAATGTATATACACGCAAGTATAATGAAATTTTTCGTTATCAACCACACACATTAAATAATGAACAAAGTAAGTTATTTTCAACATTAATACGTGCTGATGAAGGTTTTTCAACAATTTTTTCAACATATACAAATAATGATATGAAATTTAGTGATGCTATTGATGCAAAAGGTAAAAAACACCCAATTAAGAATGAAGCAGAAGCTTTTGTTCATTTAAAATCAAAAGATCGCGTTTTGCGTAAAAGCGCTTATTTATCAATGTATAGAGCATATTATGATTCACGTGAATCAATTACAAAAATGTTGTACTACAACTATTTATCATTAAATCAACAAGCAAAAGCAAAAAACTTTGATGATTATATTGCTAAAGCAGCATTTGATGATGTTGTTGACAAATCATTAATTACTTTAATTTATGATCAAGTAAAACTTTATAAAGATACAAATGAAGATTATAAAAAAGTACGAAATACATATTTAAAAAAATTAATTAAAGTTTCAAAGATCGAACCTTGA", "aa": "MTDSQLNVYTRKYNEIFRYQPHTLNNEQSKLFSTLIRADEGFSTIFSTYTNNDMKFSDAIDAKGKKHPIKNEAEAFVHLKSKDRVLRKSAYLSMYRAYYDSRESITKMLYYNYLSLNQQAKAKNFDDYIAKAAFDDVVDKSLITLIYDQVKLYKDTNEDYKKVRNTYLKKLIKVSKIEP", "aa_hexdigest": "30e3c10bc508fa441a800f2599969a01", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21161.81040000001, "isoelectric_point": 9.33909969329834 }, "id": "GPKLONFDEH_178", "locus": "GCA000006625_00138" }, { "type": "cds", "sequence": "contig_1", "start": 82145, "stop": 82339, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAGTTCTATAAATAAGTTAAAAAATAAATTAAAAAATCTAGCAAAAAAACCAATAAAAGTTTTATTTATTGAACAAAGTTTAGAAATTGATAAAGCAAAAAAAATGTTAAGTCAAGATACTTTAATTAATATCGTAGATTTGTCATCTTTTAAAACACCAGAAATTATTAATGAATGCGAAGAGATTTGA", "aa": "MLSSINKLKNKLKNLAKKPIKVLFIEQSLEIDKAKKMLSQDTLINIVDLSSFKTPEIINECEEI", "aa_hexdigest": "ea7be21d7e0f121c2c5a4909c239ebf5", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7330.667299999998, "isoelectric_point": 8.787959480285647 }, "id": "GPKLONFDEH_179", "locus": "GCA000006625_00139" }, { "type": "cds", "sequence": "contig_1", "start": 82454, "stop": 83026, "strand": "+", "frame": 2, "gene": "pta", "product": "Phosphotransacetylase", "db_xrefs": [ "COG:C", "COG:COG0280", "SO:0001217", "UniRef:UniRef50_Q9PR79", "UniRef:UniRef90_Q9PR79" ], "nt": "GTGGGTGCTAACTTGTCATCAAAAACTTGTTTTAGTAGTATCTTAAAGATTTTGAAAAATCCTAACGGTTCGATCGCATATAGTACAATGGTTATGATTAATAAACGAGAATTAATTTTTATGAGTGATTGTGCTTTAAATTTAAACTTGAATAAACAACAAATGATTGATGCAGCAAAATATTCATGCACAATTAGTAGAAATTATTTTGATGTTTTAAACAATCATGTGGGATATATTAGTTATTTAAGTCAAAATGATGTTATTTATCAAAATAAAAATTTTGATTTTATTGGCCCAATTCAATTCGATGCTGTAGTTGATTTAAAAGTTCGTAATAAAAAAGTACCTAATATCCATCAATACCATATTAATCATTATGTTTTTGATAATATAAGTATTGCAAATACTGTTTTTAAAATTTATCAAACAAAACTTAATTATTTAACAGTCGGTACATATATTAGTAATATCTTAACAAAAGTTAGTGTTATTAGTCGTTCAGCAAGTAGTGATGAAATTATTGCAACAACTTATTTATTATGCGTGGCTTTTTTAACAGATTTAACTTAA", "aa": "MGANLSSKTCFSSILKILKNPNGSIAYSTMVMINKRELIFMSDCALNLNLNKQQMIDAAKYSCTISRNYFDVLNNHVGYISYLSQNDVIYQNKNFDFIGPIQFDAVVDLKVRNKKVPNIHQYHINHYVFDNISIANTVFKIYQTKLNYLTVGTYISNILTKVSVISRSASSDEIIATTYLLCVAFLTDLT", "aa_hexdigest": "a46e3e42bc7607622aaf83adb1a7b28c", "start_type": "GTG", "rbs_motif": null, "genes": [ "pta" ], "truncated": "5-prime", "pseudogene": { "start": 82154, "stop": 83026, "observations": { "mutation": [ 82337 ], "stop": [ 82337 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR79", "query_cov": 1.0, "subject_cov": 0.6484641638225256, "identity": 0.9890000000000001, "score": 365.0, "evalue": 3.26e-126, "gene_start": 1, "gene_end": 190, "reference_start": 104, "reference_end": 293, "reference_sequence": "MLSSINKLKNKLKNLAKKPIKVLFIEQSLEIDKAKKMLSQDTLINIVDLSSFKTPEIINECEEIWYELRKLKGESRNDAQIAVNDNLNFAVCLSYLKKIDVLIVGANLSSKTCFSSILKILKNPNGSIAYSTMVMINKRELIFMSDCALNLNLNKQQMIDVAKYSCTISRNYFDVLNNHVGYISYLSQNDVIYQNKNFDFIGPIQFDAVVDLKVRNKKVPNIHQYHINHYVFDNISIANTVFKIYQTKLNYLTVGTYISNILTKVSVISRSASSDEIIATTYLLCVAFLTDLT" }, "paralog": false, "identity": 0.993103448275862, "score": 563.0, "evalue": 1.77e-206, "description": "Internal stop codon at 82337. Nonsense mutation around 82337.", "psc": { "uniref90_id": "UniRef90_Q9PR79", "gene": "Pta", "product": "Phosphotransacetylase", "uniref50_id": "UniRef50_Q9PR79", "cog_id": "COG0280", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR79", "UniRef:UniRef50_Q9PR79", "COG:COG0280", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR79" ], "product": "Phosphotransacetylase" } }, "id": "GPKLONFDEH_180", "locus": "GCA000006625_00140" }, { "type": "cds", "sequence": "contig_1", "start": 83073, "stop": 83687, "strand": "-", "frame": 2, "gene": null, "product": "HTH gntR-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B83", "UniRef:UniRef100_A0A2C9DZ67", "UniRef:UniRef50_A0A2C9DZ67", "UniRef:UniRef90_A0A2C9DZ67" ], "nt": "ATGGATCAATATAAAATAAATGAATTGATTCCTAGTGAGTATAAACTAGCAAGTTATTTTAATTGTGGACGAATCACTATTCATAATGCTTATGAAGTATTAAAAGTTTTAGGCATTGTAACAACAATCAAAGGAAGTGGTTATTATGTTTATTCAAGTATTGATCACTTTACTAACAAGGCTTTTGCTAATTTATACAAAACCATTGATAATATTAAATACGAACAATTTCTAATTGATTTTGAATATCATGGTAATAAGTTTAATTCAATCACTAAATTTAGTTTAATTAAAAATAAAAAAATAATTGCAGTATCATACTATATAACACCAAATAATATGGATTTAGAACAATTCGATACTAATAACATTAATATTAGTAAACAATTAATTCTTTCTGGTTTTGATGATTTTTTTAATGTAAAAACACAAATTAATTATGAGAATATTAATTTATTAAAATCAATTTTAGAAGATGAAAATATTAAATTTAACACACCAATTATTTTAAATAAATTAACTTCATCATCATTAAAAGTAGAATTATTAATTTTAACACACATTAGCAAAGAATATTTTGATTATGAAGAGACTAATAAATTAATTATTAATTAA", "aa": "MDQYKINELIPSEYKLASYFNCGRITIHNAYEVLKVLGIVTTIKGSGYYVYSSIDHFTNKAFANLYKTIDNIKYEQFLIDFEYHGNKFNSITKFSLIKNKKIIAVSYYITPNNMDLEQFDTNNINISKQLILSGFDDFFNVKTQINYENINLLKSILEDENIKFNTPIILNKLTSSSLKVELLILTHISKEYFDYEETNKLIIN", "aa_hexdigest": "cab5996f5fe095044e15745b86918ca6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1B83", "uniref100_id": "UniRef100_A0A2C9DZ67", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B83", "UniRef:UniRef100_A0A2C9DZ67" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DZ67", "uniref90_id": "UniRef90_A0A2C9DZ67" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DZ67", "product": "HTH gntR-type domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DZ67" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DZ67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DZ67" ], "product": "HTH gntR-type domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_181", "locus": "GCA000006625_00141" }, { "type": "cds", "sequence": "contig_1", "start": 83908, "stop": 84381, "strand": "-", "frame": 1, "gene": null, "product": "MPN555 family protein chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2NXK8", "UniRef:UniRef90_A0AAC9T382" ], "nt": "ATGAATTTTAAAAGTACCATCGTTAATAAAAAACCGATTGATTGGAAACACACAATTGTTTTAGAAGAATTAAATGTTGATCCTAAAGCATTAGAAATGCATAAAGAAAGAATTAACACAGTTTTTGCAAAACAAACTGAAGAACAACGTTCTCAACAATTACATAACATTATTGTTCGTGAAAATTTGTTTAATAAAGCAATGACATATCTTGCTGATTTTTATGAAATTGATGTTAATGAAGAAGATATTAAAGATTTAGCACCACGTATTAAACAAGCATTTGGTGTTGAAGATGAAAAGTTAGCTTATGAAATTGCACAAAAAATCATCGCTAAAGCACTAATTTTTCAAGATTTACAAAAAGAATTTACAATTGAAATTAAAGATGAAGAATTAACAAAAATACTTGAATCATACTATGAAGAGACAAACTTATCAATTCGTGACTTTAAAGAAAACAAAGCACAATGA", "aa": "MNFKSTIVNKKPIDWKHTIVLEELNVDPKALEMHKERINTVFAKQTEEQRSQQLHNIIVRENLFNKAMTYLADFYEIDVNEEDIKDLAPRIKQAFGVEDEKLAYEIAQKIIAKALIFQDLQKEFTIEIKDEELTKILESYYEETNLSIRDFKENKAQ", "aa_hexdigest": "c07c5f175cf9262b65d5ee609ce2b8c7", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 83725, "stop": 84381, "observations": { "mutation": [ 83910 ], "stop": [ 83910 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T382", "query_cov": 1.0, "subject_cov": 0.7201834862385321, "identity": 0.955, "score": 291.0, "evalue": 1.1e-98, "gene_start": 1, "gene_end": 157, "reference_start": 1, "reference_end": 157, "reference_sequence": "MNFKSTIINKKPIDWKHTIVLEELSVDPKALEMHKERINTVFAKQTEEQRAQQLHNIIVRENLFNKAMTYLADFYEIDVNEEDVKDLAPRIKQAFGVEDEKLAYEISQKIIAKALIFQDLQKEFNIEIKDDELTKILESYYEETNLSIRDFKENKAQWEAAKSTLLEEKTTAFIVDKFDRDLSILEANIRKKIAEQMELDKKIKEVQDNSKAKQNADK" }, "paralog": false, "identity": 0.9495412844036697, "score": 409.0, "evalue": 2.49e-147, "description": "Internal stop codon at 83910. Nonsense mutation around 83910.", "psc": { "uniref90_id": "UniRef90_A0AAC9T382", "product": "MPN555 family protein chaperone", "uniref50_id": "UniRef50_A0A9E2NXK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T382", "UniRef:UniRef50_A0A9E2NXK8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9E2NXK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2NXK8" ], "product": "Trigger factor C-terminal domain-containing protein" } }, "id": "GPKLONFDEH_182", "locus": "GCA000006625_00142" }, { "type": "cds", "sequence": "contig_1", "start": 84472, "stop": 85347, "strand": "-", "frame": 1, "gene": "fepC", "product": "Iron(III) dicitrate transport ATP-binding protein", "db_xrefs": [ "COG:COG1120", "COG:PH", "RefSeq:WP_006688512.1", "SO:0001217", "UniParc:UPI00000C1B85", "UniRef:UniRef100_Q9PR76", "UniRef:UniRef50_Q9PR76", "UniRef:UniRef90_Q9PR76" ], "nt": "ATGTTTAAAATTTTTGCTAAATACTTTTGCAATCATCAATTAGATCGGAATCAAATTATTAATGCAAGTAAAAACGACGCTTTAATTATTAATAATTTATCTTATAGATATCACAAAAAACTTCCTGAAGTTTTAAAAAATTTATCATTTAGTATTAAAAAAAATTCATTTACAACAATTTTAGGACCAAATGGATGTGGTAAGTCTACAACAGCGAAAGCGATTGTTAAATTATTAAAACTTAAAAAAAATATTATTAAAGTGTTTGATAGAAACATTAATGAATTAAGTTTTAAACAATTAGCACAATTAATTTCTTACATTCCTCAAAGTATTGAAATTCCCCAAGGCACTCGTGTTATTGATTTTATTACGTTTGGTCGTAATCCATATTTAGGATTTAGTGGTATTTTAGGAAAAGAAGATAAATTAATTATTGATTTAGTAATTAATGAAATGCAACTGCACGATTTAAAAGAAAAATTTATGCAAGAATTATCTGGTGGTCAGCGCCAAAAAGTTATTTTAGCATTGTGTTTAGTTCAAGATACTCCAATTATTTTATTAGATGAACCAACTACATATTTAGATATTAAAAATCAATATGAATTACTTGAATCACTAAAAAAATTACAAATTTTACGTCAAAAAACAATTATTGCAATTTTACATGATATTAATCAAGCTATTCAATATAGTGATGAAGTTTTTGTCTTAAAAAATGGGCAAATCTATGCAAATGGTAATCCAAATGAAGTTATTACTAAAGAGTTATTAAAAAACGTTTATAACATTGATGCTCAAATTGATATTGTTGATAATCAAAAAATTGTTCATAAGATTAAAGTTAAAGATTATTTATCAAATAGTGAATAA", "aa": "MFKIFAKYFCNHQLDRNQIINASKNDALIINNLSYRYHKKLPEVLKNLSFSIKKNSFTTILGPNGCGKSTTAKAIVKLLKLKKNIIKVFDRNINELSFKQLAQLISYIPQSIEIPQGTRVIDFITFGRNPYLGFSGILGKEDKLIIDLVINEMQLHDLKEKFMQELSGGQRQKVILALCLVQDTPIILLDEPTTYLDIKNQYELLESLKKLQILRQKTIIAILHDINQAIQYSDEVFVLKNGQIYANGNPNEVITKELLKNVYNIDAQIDIVDNQKIVHKIKVKDYLSNSE", "aa_hexdigest": "3501a4b70de601a35589842b2912b478", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00000C1B85", "ncbi_nrp_id": "WP_006688512.1", "uniref100_id": "UniRef100_Q9PR76", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B85", "RefSeq:WP_006688512.1", "UniRef:UniRef100_Q9PR76" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR76", "uniref90_id": "UniRef90_Q9PR76" }, "psc": { "uniref90_id": "UniRef90_Q9PR76", "gene": "FepC", "product": "Iron(III) dicitrate transport ATP-binding protein", "uniref50_id": "UniRef50_Q9PR76", "cog_id": "COG1120", "cog_category": "PH" }, "pscc": { "uniref50_id": "UniRef50_Q9PR76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR76" ], "product": "Iron(III) dicitrate transport ATP-binding protein" }, "genes": [ "fepC" ], "id": "GPKLONFDEH_183", "locus": "GCA000006625_00143" }, { "type": "cds", "sequence": "contig_1", "start": 85352, "stop": 85609, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTTATTAGCAGGATTAGAAATTGTTTTAGTTGGTTATATAGCTTTATTAGGAGTTATTGGCGCTAGCGTTGCACGTCAATTATTTGGTAATAGAACAGGTATTACAATGTTTGCAGCATTTTTAATCGGCGCAACTTTAGTGTGTTTTGCGTTATTAATTTCGGTTAATTTTAATACACAAGTACCAGTTGGTTTTTTATCAACAACAATTATTACACCTTATTTTATGTATCTAATTGTGAGAGGAAAATAA", "aa": "MALLAGLEIVLVGYIALLGVIGASVARQLFGNRTGITMFAAFLIGATLVCFALLISVNFNTQVPVGFLSTTIITPYFMYLIVRGK", "aa_hexdigest": "92d297ec9efb3e15d61c63c2d8442133", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9056.890599999993, "isoelectric_point": 9.622825050354006 }, "id": "GPKLONFDEH_184", "locus": "GCA000006625_00144" }, { "type": "cds", "sequence": "contig_1", "start": 85709, "stop": 86422, "strand": "-", "frame": 3, "gene": "fepD", "product": "Hemin transport system permease", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_Q9PR75", "UniRef:UniRef90_Q9PR75" ], "nt": "ATGAAAATTAATCAAAAAACGAATTTAAAGCAAAAGCATTTTTATTTAATTAAAAATCTTAATTATAAACAATCTGTAATCATTAAAATGGCAGGACTAATTCTCATTGCCATTTGTTTTGTTTTTTTAACATTAGCAATTTATTTTGATACCACTAATGGTTTTGAAGCATCGATCAATCAATTTAATTTTAATATTCGTGGTCCTAAAACTTTAATGGCCTTTTTTTCAGGAGGAGCAATTGCTGTAGCTGGATTATTATTACAAAAAGTTACACGTAATCATTTAGCAGATACGTCAGTTTTAGGTATTGGTTCATTAAACATCATTTTAATGACAGTATATTTAATTCTTATTAAAGATAAAACAAGCAGTTATTTAAATCCTGAAATGCGAATTTTACCCTTGGTGATGTTTTTATCATCAATTTTTGGAACAAGTATTGTTTATACATTATCAAAATTAGGAAGAGCTAATAATGAAAAATTTATTATTATCGGAATTGCTTTGAATTTTTTATTTGAAGCGATTTCCGTGGTTTTAATTAATCCTGCTAATAATTCATTGTTCTCCAATCAAGATAAAAATTTAATGATTGCTTTTGAAAATATCAAAAATTATACTTTAGGAATTATTCCAAATTCGCAATATATTGATTTATCATTAGTAATCTCTTGCTCAGTATTAATTGCATTAACATTGATTCCAATTTGA", "aa": "MKINQKTNLKQKHFYLIKNLNYKQSVIIKMAGLILIAICFVFLTLAIYFDTTNGFEASINQFNFNIRGPKTLMAFFSGGAIAVAGLLLQKVTRNHLADTSVLGIGSLNIILMTVYLILIKDKTSSYLNPEMRILPLVMFLSSIFGTSIVYTLSKLGRANNEKFIIIGIALNFLFEAISVVLINPANNSLFSNQDKNLMIAFENIKNYTLGIIPNSQYIDLSLVISCSVLIALTLIPI", "aa_hexdigest": "5d7eae396c0675dcd627e8586de80ca2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "fepD" ], "truncated": "5-prime", "pseudogene": { "start": 85406, "stop": 86422, "observations": { "mutation": [ 85711 ], "stop": [ 85711 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR75", "query_cov": 1.0, "subject_cov": 0.6657303370786517, "identity": 0.92, "score": 409.0, "evalue": 1.75e-141, "gene_start": 1, "gene_end": 237, "reference_start": 1, "reference_end": 237, "reference_sequence": "MKINYKTKLKQHHFYLIKNLNYKQFAIIKMAGLIAIALCFIFLTLAIYYDTTNGFDVSINQFNFNIRGPKTLMAFFSGGAIAVAGLLLQKVTRNHLADTSVLGIGSLNIILMTFYFILIKDKTSSYLNPEMRILPLIMFLSSILGTSIVYTLSKLGKANNEKFIIIGIALNFLFEAVSVVLINPANNSLFSNQDKNLMIAFENIKNYTLGIIPNSQYIDLSLVISCSILITLTLIPIWFLRKKIDLYETSAALASTTGINVERLKISIYFLVALLAGLEIVLVGYVALLGVIGASVARQLFGNKTGITMFAAFLIGAILVCFALLISVNFNTQVPVGFLSTTIITPYFMYLIVRGK" }, "paralog": false, "identity": 0.9260355029585798, "score": 604.0, "evalue": 4.03e-221, "description": "Internal stop codon at 85711. Nonsense mutation around 85711.", "psc": { "uniref90_id": "UniRef90_Q9PR75", "gene": "FepD", "product": "Hemin transport system permease", "uniref50_id": "UniRef50_Q9PR75", "cog_id": "COG0609", "cog_category": "P", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR75", "UniRef:UniRef50_Q9PR75", "COG:COG0609", "COG:P" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR75" ], "product": "Hemin transport system permease" } }, "id": "GPKLONFDEH_185", "locus": "GCA000006625_00145" }, { "type": "cds", "sequence": "contig_1", "start": 86465, "stop": 86572, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAGATTCTGTGATTAAAATTATTAATAAAACAATTGATCCGCAATTAATCCAACAAAATAAACAATTGTTAAAAGAAGCTAATGATTGGGGTTCATATTGA", "aa": "MILDSVIKIINKTIDPQLIQQNKQLLKEANDWGSY", "aa_hexdigest": "72fd0c41a4cb5f6a038fd320d310d612", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4071.694300000001, "isoelectric_point": 5.91923542022705 }, "id": "GPKLONFDEH_186", "locus": "GCA000006625_00146" }, { "type": "cds", "sequence": "contig_1", "start": 86819, "stop": 87358, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCGATTCATTGCTTTATTTATATGATATTGCTTATGCAACAACAAAATATGCATACTCAGAAGAAGCAAAAATTGATTTTAAAGATAATTTAGCAAAATTAGAACCATTAAAAAAAGCGTTATTTAATGCTAATCAAATTGCTAATAATTTAATTAATCGCTTAAACAAAATGCGTTTGTATTTTCAAGCAATTGGCGTTGTTGATAAAAATTATAATCCTAATATAAAACAATTTGATAATACTAATTCCAAAACTTTAGGCTTATTAACTACATCACAAAATAGTGGTTCTTCAACTTTACAAACTCAATCTAAATACGGTTTTTTATATTATGATTTAGGGTTTAAAGCACCTAAACCAATCTTAAAAAAGGACGAATGGACTGAATTATTAGAAGATGTTAACGGCAAACAATTATTATGTCATAGACACGGAAATGGACAAATTCATTGTTTTGGTGGTGATAATCAAGATGGGACACAAATTGATAAAAACATTGTTGGTTCTCTATTTAATATGGATGATAATGGTTGA", "aa": "MRDSLLYLYDIAYATTKYAYSEEAKIDFKDNLAKLEPLKKALFNANQIANNLINRLNKMRLYFQAIGVVDKNYNPNIKQFDNTNSKTLGLLTTSQNSGSSTLQTQSKYGFLYYDLGFKAPKPILKKDEWTELLEDVNGKQLLCHRHGNGQIHCFGGDNQDGTQIDKNIVGSLFNMDDNG", "aa_hexdigest": "c0f47a5e6d51d3d4298013c8e15328a7", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20316.668799999992, "isoelectric_point": 7.698693656921387 }, "id": "GPKLONFDEH_187", "locus": "GCA000006625_00147" }, { "type": "cds", "sequence": "contig_1", "start": 87389, "stop": 87523, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACAAAAAAAGTTAGGGAATCAGGATTAAATAAATTAAATTATGATCCTTTTTTATCAACTACAAAACAAGATAGTTATGTTACATCAATTCTACAACACCATCCTATTTATGAACAAAATATGCGATGA", "aa": "MLTKKVRESGLNKLNYDPFLSTTKQDSYVTSILQHHPIYEQNMR", "aa_hexdigest": "88988f544e609fa9f22c254ee38d8b9b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5225.909799999999, "isoelectric_point": 9.223765754699706 }, "id": "GPKLONFDEH_188", "locus": "GCA000006625_00148" }, { "type": "cds", "sequence": "contig_1", "start": 87575, "stop": 87958, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATAATAGTCATTTTGAACGTGCAAAAAAAACATTAGTTGCACATGGTGGCCCGATATCAAATCAAAAAACTGCTATTCCTGTTGATTTTAAAATGAGTCGTGATTTTTATTTAACCTTAAATAAAAATGACATTTTAAATTTTAAAAACGATCCTAACTCATTATTACGTAAAAGTCTATTGTTAGGTTTAGAGTATAAGGATAAAGAAAAAGGTGAATTAAACGAACTTTATAATTATTCCTATTTTGCTGATAAATTAAACAAAAACTATTTTAATGCTGAAACATTTGATGGTATTAATTTTAATAATTCAGCTTTTAAAAAAAGAATTTTAGAAGGTAATGATATACCTATTTTTTATACTGAAACAAAATGA", "aa": "MIDNSHFERAKKTLVAHGGPISNQKTAIPVDFKMSRDFYLTLNKNDILNFKNDPNSLLRKSLLLGLEYKDKEKGELNELYNYSYFADKLNKNYFNAETFDGINFNNSAFKKRILEGNDIPIFYTETK", "aa_hexdigest": "c89df5d4994d11d6186424f341a14409", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14799.562699999982, "isoelectric_point": 8.6880334854126 }, "id": "GPKLONFDEH_189", "locus": "GCA000006625_00149" }, { "type": "cds", "sequence": "contig_1", "start": 88193, "stop": 88408, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAAGAAAACAAAAAAATATTTAATTAGTATGTTGGCAACAATTTCTATTGTAATTGGTACAACAACAATTATTAGTTCATGTACAAAAAAAACATCTGCAAACGTTCAAGAGCAAATCAAATACGATATAATGCCAACTTATACTTCTCAGGCTGATAATTTATTAGCGTTAGGAATTACACCTGATTATTATCCTCAACAAATGTATTGA", "aa": "MHKKTKKYLISMLATISIVIGTTTIISSCTKKTSANVQEQIKYDIMPTYTSQADNLLALGITPDYYPQQMY", "aa_hexdigest": "66288898504da054bab17e70581ec89f", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8008.285200000004, "isoelectric_point": 9.099406242370605 }, "id": "GPKLONFDEH_190", "locus": "GCA000006625_00150" }, { "type": "cds", "sequence": "contig_1", "start": 88851, "stop": 89147, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGATTAATAATTATACAATTACCTTGGCAACCATTTTAATTGGTAACACATTAGTAAATACAGCATCATCAACAATCGCAGGTTTATTTTTTGCAGGTATAATGAAATCTTTTAGTACAACAAGTAACGCTGAAGCTATTGGTACAGGAATTGCAACAGGAATTATAACTTTAATTGTTTTAATATTTGGCGAATTTATTCCTAAAAATTTTGCTAAGAAAAATCCGATTAAAGTTCTTAAATTAGTAGCTTATCCCTTAAATTGTTTCTATATACTTTTTTGGCCATTAACTTGA", "aa": "MINNYTITLATILIGNTLVNTASSTIAGLFFAGIMKSFSTTSNAEAIGTGIATGIITLIVLIFGEFIPKNFAKKNPIKVLKLVAYPLNCFYILFWPLT", "aa_hexdigest": "269420fe648ad2d44afe47ee4122e33e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 97, "aa_cov": 0.9897959183673469, "hmm_cov": 0.9278350515463918, "evalue": 4.342978870177254e-27, "score": 90.50971221923828, "start": 1, "stop": 97 } ], "seq_stats": { "molecular_weight": 10591.528599999983, "isoelectric_point": 9.519095420837402 }, "id": "GPKLONFDEH_191", "locus": "GCA000006625_00151" }, { "type": "cds", "sequence": "contig_1", "start": 89406, "stop": 89738, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00571.33" ], "nt": "TTGGTTGTAGTCAATAAAAACGATGAGGTTGTTGGTATTATTTATTTAAAAAAAATTATTAAATATCTTCTTAACGAACCTAATAAAATCATTAGAACTATCATGCAAACACCTTTATTAATAAACCAAAAAGATACATTATATGATGGTTTAAGAACAATGCAATTTCAAAGAATTCATTTAGGAATTGTTGTTGATAATATTACTGAAAAAAATCCTATAGGAATCATTACATTAGAAAACATTACTGAAGAATTAACTGGTAATGTTTATGATGAAACTGATAATGAGAACGAAATAAAAGAAATTCAAATCGTTAATGAAACAACTTGA", "aa": "MVVVNKNDEVVGIIYLKKIIKYLLNEPNKIIRTIMQTPLLINQKDTLYDGLRTMQFQRIHLGIVVDNITEKNPIGIITLENITEELTGNVYDETDNENEIKEIQIVNETT", "aa_hexdigest": "818c81211b1efa01a76b2b574e8b9ba8", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00571.33", "name": "CBS", "length": 59, "aa_cov": 0.509090909090909, "hmm_cov": 0.9322033898305084, "evalue": 1.942827409789684e-11, "score": 39.74420166015625, "start": 31, "stop": 86 } ], "seq_stats": { "molecular_weight": 12712.537099999989, "isoelectric_point": 4.708337593078612 }, "id": "GPKLONFDEH_192", "locus": "GCA000006625_00152" }, { "type": "cds", "sequence": "contig_1", "start": 89820, "stop": 89969, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAAATATATTTCTAAAATTAATAAAAATCGCGCATTTAAAAACCGCCAATTAATTAACACTCCTAATATGTATATTGAAACTTTTAAAGATGTAAGAACAAATCAATTTGCCTTTTTAATTGAAAAAAAGATTAATCAATCCTAA", "aa": "MGKYISKINKNRAFKNRQLINTPNMYIETFKDVRTNQFAFLIEKKINQS", "aa_hexdigest": "5800907ead17b0865abd405b8f6514e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5895.813700000001, "isoelectric_point": 10.288783454895018 }, "id": "GPKLONFDEH_193", "locus": "GCA000006625_00153" }, { "type": "cds", "sequence": "contig_1", "start": 89987, "stop": 90649, "strand": "-", "frame": 3, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "SO:0001217", "UniRef:UniRef50_Q9PR72", "UniRef:UniRef90_Q9PR72" ], "nt": "TTGGATAAATTAGATGAAATTTTAAAACTATTTGATGATGCGAATAATGAAAATTTTCATAAAGACCGCTATTGTTCAAATTTTTTAATTGGTGCAATGTTGTCTGGTGGTTCAATTGCACACCCACTTGAAAACTATCATTTAGAAATTCGCTGTGATTCAAATAATTACATCCCATTATTAACAAAGGCTTTAGCCCGTTATGGTTTAGAATATAAAATTATTTATCGTAATAAAAAAACCATTGTTTATTTTAAAAAATCAGAGATAATCAGTGATTTTTTAAAAGCGATCCGTACGCAAAATTCGTTATTTGAATTTGAGAATATTCGTATCCAACGTGATTTTAATAATCAACAACAACGTTTAAATAATTTAGATATTTCTAACCTTTCTAAAAGTTCTAAAGCAGGGGTTTTGACTAAAGAAATGATTTTAGAAATAAAAAAAAACCACGATGAATTTTGCAAACAAAGTACTAAATTTTTAAAGTATTGTGAACTACGAGTACAAAATCCAGACTGTTCATTAAATGAACTTTCTTGTTTATTAAAACAGATGTTTAATATTGAAATTTCAAAAAGTGGTTTAAATCATTTTAATTCTCGTATTAAAGAAATGTATGAAGAATTAAAATTAAAAAAACTAGTTTTTAAAAACTAG", "aa": "MDKLDEILKLFDDANNENFHKDRYCSNFLIGAMLSGGSIAHPLENYHLEIRCDSNNYIPLLTKALARYGLEYKIIYRNKKTIVYFKKSEIISDFLKAIRTQNSLFEFENIRIQRDFNNQQQRLNNLDISNLSKSSKAGVLTKEMILEIKKNHDEFCKQSTKFLKYCELRVQNPDCSLNELSCLLKQMFNIEISKSGLNHFNSRIKEMYEELKLKKLVFKN", "aa_hexdigest": "535f555a383b4044bdcfe95e331949dc", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "whiA" ], "truncated": "3-prime", "pseudogene": { "start": 89987, "stop": 90949, "observations": { "mutation": [ 90658, 90802 ], "stop": [ 90658, 90802 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR72", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.995, "score": 435.0, "evalue": 1.59e-152, "gene_start": 1, "gene_end": 220, "reference_start": 111, "reference_end": 330, "reference_sequence": "MPKSTLQEIIDNDSNKFTFSEFVKEDIYTNTDYSSTDYKVILYSFFRNNLTIKIGQKLTWIIKSQNLQIIEFILNGLDYFNNLNIEYEIMFEPDHLNKTRTNYSLLLWGDLDKLDEILKLFDDANNENFHKDRYCSNFLIGAMLSGGSIAHPLENYHLEIRCDSNNYIPLLTKALARYGLEYKIIYRNKKTIVYFKKSEIISDFLKAIRTQNSLFEFENIRIQRDFNNQQQRLNNLDISNLSKSSKAGVLTKEMILEIKKNHDEFCKQSTKFLKYCELRVQNPDCSLNELSCLLKQMFNIEISKSGLNHFNSRIKEMYEELKLKKLVFKN" }, "paralog": false, "identity": 0.99375, "score": 636.0, "evalue": 2.6e-234, "description": "Internal stop codon at 90658, 90802. Nonsense mutation around 90658, 90802.", "psc": { "uniref90_id": "UniRef90_Q9PR72", "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_Q9PR72", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR72", "UniRef:UniRef50_Q9PR72", "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR72" ], "product": "Probable cell division protein WhiA" } }, "id": "GPKLONFDEH_194", "locus": "GCA000006625_00154" }, { "type": "cds", "sequence": "contig_1", "start": 90800, "stop": 90979, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAGAGTACATTACAAGAAATAATTGATAATGATTCTAACAAATTCACGTTTAGTGAATTTGTTAAAGAAGATATTTATACTAACACTGATTATTCATCCACAGACTATAAAGTTATTTTATATTCGTTTTTTCGTAATAATTTAACCATTAAAATTGGTCAAAAACTAACTTGA", "aa": "MPKSTLQEIIDNDSNKFTFSEFVKEDIYTNTDYSSTDYKVILYSFFRNNLTIKIGQKLT", "aa_hexdigest": "a0fa39056c81b580a0ac0da3fba9fc49", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6966.738600000001, "isoelectric_point": 5.086430549621581 }, "id": "GPKLONFDEH_195", "locus": "GCA000006625_00155" }, { "type": "cds", "sequence": "contig_1", "start": 90948, "stop": 91874, "strand": "-", "frame": 2, "gene": "trxB", "product": "Thioredoxin reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "EC:1.8.1.9", "GO:0004791", "GO:0005737", "SO:0001217", "UniRef:UniRef50_Q9PR71", "UniRef:UniRef90_Q9PR71" ], "nt": "ATGAATCAAGAAGTTTACGATTTAGTAATCATTGGCGCAGGTCCAGCCGGTTTAGCTGCTGCTGTTTATGCTAAACGTTCTGGATTAAATGTGATCATTGTTGAAAAACAATTCCCAGGAGGAAAAATAGCATTAACATCAAATGTAGAAAATTATTTAGGTATCAATAGTATTCCAGGACCTGAATTGGCTTATAAAATGTACGAACAAGTCTTAAATTTAAATGTTTCTATTATTTATGAAGCTGCTGATGAAATATCTTTAAAAGAAAAATATAAAAAAATTAAATTAACTACACAAACATTAATTACTAAAACTGTAATTATTGCAACAGGAACAGAAAATAGACGGTTAAACATTTTAGGTGAATTAGAATTTGAAAACAAGGGAATTAGTTATTGCGCTATTTGTGATGGACCACTTTATAAAAATAAAGCTGTCTCTGTGATTGGATCTGGTAACTCTGCTGTGGAAGAGGCCATATATTTAGCTACAATTGCTAAAGAAGTTCATTTAATTGCCAATAAACCACAATTTAAAGCAGAACAACAATTAGTACAAATTGCAAATAACACACCTAATATAAAAATTTATTACAATAAACAAACATTCGAATTTTTTGGTCATCAGTTTTTAGAAGGTTTAAAATTTAGAGATTTAATCACAAACGAAGTAACTACACTAAATATTGAAGCTAATTTCACTTTTATTGGCCTATTGCCTTCAAGAATTAATACAAATAATTTATGTATTTTTAATGAGGTAAACGGATTTATTACAACTGATAAAAATATGCAAACTAGTGTTTGTGGTATTTTTGCAGCCGGTGATATTGTTGATAAAAATGTTCGACAAATTGCGACTGCTACAAATGATGGAGTCATTGCAGCACTTTATGCCAAAGAGTACATTACAAGAAATAATTGA", "aa": "MNQEVYDLVIIGAGPAGLAAAVYAKRSGLNVIIVEKQFPGGKIALTSNVENYLGINSIPGPELAYKMYEQVLNLNVSIIYEAADEISLKEKYKKIKLTTQTLITKTVIIATGTENRRLNILGELEFENKGISYCAICDGPLYKNKAVSVIGSGNSAVEEAIYLATIAKEVHLIANKPQFKAEQQLVQIANNTPNIKIYYNKQTFEFFGHQFLEGLKFRDLITNEVTTLNIEANFTFIGLLPSRINTNNLCIFNEVNGFITTDKNMQTSVCGIFAAGDIVDKNVRQIATATNDGVIAALYAKEYITRNN", "aa_hexdigest": "9f67ecbfb6ac62f0b83559f0e2646b49", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_Q9PR71", "query_cov": 1.0, "subject_cov": 0.9967637540453075, "identity": 1.0, "score": 602.0, "evalue": 1.39e-217, "valid": true, "gene": "trxB", "product": "Thioredoxin reductase", "ec_ids": [ "1.8.1.9" ], "uniref50_id": "UniRef50_Q9PR71", "cog_id": "COG0492", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0004791" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR71" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "GPKLONFDEH_196", "locus": "GCA000006625_00156" }, { "type": "cds", "sequence": "contig_1", "start": 91893, "stop": 92336, "strand": "-", "frame": 2, "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "db_xrefs": [ "COG:COG0682", "COG:M", "EC:2.5.1.145", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158", "SO:0001217", "UniRef:UniRef50_Q9PR70", "UniRef:UniRef90_Q9PR70" ], "nt": "ATGCCAGCTGTTTTCGAAGGAATGAAACATTATTTCATTGAGGGCAATAAAACTCTATTTACAATTTATCAACCTTTATTTCTATATGAATCATTCTTTAATGTAATTGTTTTTGTTTTTATTTATTTTGGCTTGTCTTACATTAAACAATTAAAAATAGGCTTTATTTCAATGTCATATTTTTTCTTTTATGGTGTTACTCGTTTTAGTACGGAGTCAGCTCGTGCTCCACAGTTTAGTTTTGAAGGAACTTATATAATTAATTCATTGTTATTAATCTTTGGTGTATTAGGTGCATTATATGTACAATTTATAGCGCCACTTTTAAGAAAAAAATTCTTATTAGATGCAATTATTGAAATGTTTTATAAAAAGAAAGACCAAATTCATAAATTTGGAGAATTAAGAAAGCCAGAAGAATTTTTATTTTATTGTCATAAATAA", "aa": "MPAVFEGMKHYFIEGNKTLFTIYQPLFLYESFFNVIVFVFIYFGLSYIKQLKIGFISMSYFFFYGVTRFSTESARAPQFSFEGTYIINSLLLIFGVLGALYVQFIAPLLRKKFLLDAIIEMFYKKKDQIHKFGELRKPEEFLFYCHK", "aa_hexdigest": "e0337b677e5a5b79cbb2afb73a6b656a", "start_type": "ATG", "rbs_motif": null, "genes": [ "lgt" ], "truncated": "3-prime", "pseudogene": { "start": 91893, "stop": 92636, "observations": { "mutation": [ 92351, 92411, 92468 ], "stop": [ 92351, 92411, 92468 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR70", "query_cov": 1.0, "subject_cov": 0.4388059701492537, "identity": 1.0, "score": 289.0, "evalue": 4.14e-96, "gene_start": 1, "gene_end": 147, "reference_start": 189, "reference_end": 335, "reference_sequence": "MQLEIINPESTLVNDVVAHRVAFSIGSNFNIYWYGIIFVCGFLIAILTYSLRLKFHYKVPYDPGFYYIFLAIPMTIIGARLWSLAIGDAKDFFDFRSGGLAIQGGVIAGVLSAAVYFPLILRMPKYHIRDIDADGNVVIRQPSMWIYADAIIPTILIGQALGRWGNFINGEIFGAESTVNDLQWLKKAMPAVFEGMKHYFIEGNKTLFTIYQPLFLYESFFNVIVFVFIYFGLSYIKQLKIGFISMSYFFFYGVTRFSTESARAPQFSFEGTYIINSLLLIFGVLGALYVQFIAPLLRKKFLLDAIIEMFYKKKDQIHKFGELRKPEEFLFYCHK" }, "paralog": false, "identity": 0.9878542510121457, "score": 483.0, "evalue": 7.08e-175, "description": "Internal stop codon at 92351, 92411, 92468. Nonsense mutation around 92351, 92411, 92468.", "psc": { "uniref90_id": "UniRef90_Q9PR70", "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "ec_ids": [ "2.5.1.145" ], "uniref50_id": "UniRef50_Q9PR70", "cog_id": "COG0682", "cog_category": "M", "go_ids": [ "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR70", "EC:2.5.1.145", "UniRef:UniRef50_Q9PR70", "COG:COG0682", "COG:M", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR70" ], "product": "Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase" } }, "id": "GPKLONFDEH_197", "locus": "GCA000006625_00157" }, { "type": "cds", "sequence": "contig_1", "start": 92466, "stop": 92585, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGCTGGGGTATTAAGTGCTGCAGTTTATTTTCCTCTTATTTTGCGCATGCCTAAATATCACATTCGAGATATTGATGCTGATGGCAATGTTGTTATTCGCCAACCTAGTATGTGA", "aa": "MIAGVLSAAVYFPLILRMPKYHIRDIDADGNVVIRQPSM", "aa_hexdigest": "56a1b836fc876ec43ec65d0842caca8d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4372.185400000001, "isoelectric_point": 8.273501777648928 }, "id": "GPKLONFDEH_198", "locus": "GCA000006625_00158" }, { "type": "cds", "sequence": "contig_1", "start": 92802, "stop": 92900, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAGAAATTATTAATCCAGAAAGCACATTAGTTAATGATGTTGTTGCACATCGAGTAGCTTTTAGTATTGGTAGCAATTTTAATATATATTGA", "aa": "MQLEIINPESTLVNDVVAHRVAFSIGSNFNIY", "aa_hexdigest": "beb5310543600254321f06f3ac402bf0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3592.0397999999996, "isoelectric_point": 4.649395561218263 }, "id": "GPKLONFDEH_199", "locus": "GCA000006625_00159" }, { "type": "cds", "sequence": "contig_1", "start": 92901, "stop": 93527, "strand": "-", "frame": 2, "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "db_xrefs": [ "COG:COG1493", "COG:T", "EC:2.7.11.-", "EC:2.7.4.-", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109", "KEGG:K06023", "SO:0001217", "UniRef:UniRef50_Q9PR69", "UniRef:UniRef90_Q9PR69" ], "nt": "TTGTTAAAATTAGCAGAGCGCTATAAAATTCCAATTGTAGAGGTTAAATATAGTTTTTATGAATTAAATTTTACAATTAATAGTTACATTAGTCAAAAACTTTCACACCAATCATTAGTTCACGGAACATTATTATCTGTTTTTGGAATCGGCGTTATTTTAATGGGGGAATCAGGTGTTGGTAAATCTGAGTTAGCAATTGAATTAGTTAAAAAAGGTCATATTTTTGTAGGTGATGATGCTATTTTAGTTAATAGAATTGGAGGTAATCTATATGGAAGAGCTGAAGATTCGACTAAAGATTTTATTGAAATTAGAGGTTTAGGTATTATGAATTTTTCACGTTCTTTCGGTATTGAACGAATGATTGAATCAACAAAAATTGAAATAGTTATTGAATTAATAAAACCAGCTAAACATGAAAAAATCAAGTTTGAACGTTTTGGTCGTGAAATTCAACACAAAGAATTTTTAGAAACAAAAATAGCCCATTATTATATTCCGGTTATCGAAGGAAGAAGTATTTCAGATATCATTGAGACAGCCATTACTGATTATAAATTAAAAACATCAGGTTATAATTCAGCTGAAGAATTTATTTTACAAATTAATAAAAAGGGGATTTAA", "aa": "MLKLAERYKIPIVEVKYSFYELNFTINSYISQKLSHQSLVHGTLLSVFGIGVILMGESGVGKSELAIELVKKGHIFVGDDAILVNRIGGNLYGRAEDSTKDFIEIRGLGIMNFSRSFGIERMIESTKIEIVIELIKPAKHEKIKFERFGREIQHKEFLETKIAHYYIPVIEGRSISDIIETAITDYKLKTSGYNSAEEFILQINKKGI", "aa_hexdigest": "4e3cb5ccaf8df3140c15aee194c4af40", "start_type": "TTG", "rbs_motif": null, "genes": [ "hprK" ], "truncated": "3-prime", "pseudogene": { "start": 92901, "stop": 93827, "observations": { "mutation": [ 93671 ], "stop": [ 93671 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR69", "query_cov": 1.0, "subject_cov": 0.6753246753246753, "identity": 0.995, "score": 398.0, "evalue": 1.31e-138, "gene_start": 1, "gene_end": 208, "reference_start": 101, "reference_end": 308, "reference_sequence": "MEIRGKLFVSQVVRKFNLNVVANSDYIDREISTTGITRVGFELAGEILFKEIWNIVYFGSKESNYFNKFSETIISKKLGKILDLNPPLIIFGKNFKHAGILLKLAERYKIPIVEVKYSFYELNFTINSYISQKLSHQSLVHGTLLSVFGIGVILMGESGVGKSELAIELVKKGHIFVGDDAILVNRIGGNLYGRAEDSTKDFIEIRGLGIMNFSRSFGIERMIESTKIEIVIELIKPAKHEKIKFERFGREIQHKEFLETKIAHYYIPVIEGRSISDIIETAITDYKLKTSGYNSAEEFILQINKKGI" }, "paralog": false, "identity": 0.9967532467532467, "score": 595.0, "evalue": 9.99e-219, "description": "Internal stop codon at 93671. Nonsense mutation around 93671.", "psc": { "uniref90_id": "UniRef90_Q9PR69", "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "ec_ids": [ "2.7.11.-", "2.7.4.-" ], "uniref50_id": "UniRef50_Q9PR69", "cog_id": "COG1493", "cog_category": "T", "kegg_orthology_id": "K06023", "go_ids": [ "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR69", "EC:2.7.11.-", "EC:2.7.4.-", "UniRef:UniRef50_Q9PR69", "COG:COG1493", "COG:T", "KEGG:K06023", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR69" ], "product": "HPr kinase/phosphorylase" } }, "id": "GPKLONFDEH_200", "locus": "GCA000006625_00160" }, { "type": "cds", "sequence": "contig_1", "start": 93669, "stop": 93827, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATTCGTGGTAAATTATTTGTTAGTCAAGTTGTTAGAAAGTTTAATTTAAACGTTGTTGCAAATTCTGACTATATTGATCGTGAGATTTCTACAACAGGAATTACACGTGTTGGATTTGAATTAGCTGGCGAAATTTTGTTTAAAGAGATTTGA", "aa": "MEIRGKLFVSQVVRKFNLNVVANSDYIDREISTTGITRVGFELAGEILFKEI", "aa_hexdigest": "8a049a862463c58cf04c72de3b4eb0b2", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5917.787799999996, "isoelectric_point": 6.133973121643066 }, "id": "GPKLONFDEH_201", "locus": "GCA000006625_00161" }, { "type": "cds", "sequence": "contig_1", "start": 93842, "stop": 94096, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCAATATTATTTAATTTACTACTTTTTAATTGATCATAAAATTATTAATCCAAGTGAAAATAAAATCATTTCATTAATCAAATTCTTTGGTAAACAAATTAATAAAGAAAAAGCTTATCGTGTTCAAGAAAATATTTATATGCATGTTAATGATGATGAATTAATTAGTTTAATATCATACAATAAAAGCGATAATATTAGCAACTCTAATGTTGATATTATCGATGATCATAATGTTATTGAGAAATAG", "aa": "MVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDELISLISYNKSDNISNSNVDIIDDHNVIEK", "aa_hexdigest": "08560dc6deea70338c689e83c5dac7cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10027.310999999994, "isoelectric_point": 5.458271217346191 }, "id": "GPKLONFDEH_202", "locus": "GCA000006625_00162" }, { "type": "cds", "sequence": "contig_1", "start": 94241, "stop": 94753, "strand": "-", "frame": 3, "gene": "tilS", "product": "tRNA(Ile)-lysidine synthase", "db_xrefs": [ "COG:COG0037", "COG:J", "EC:6.3.4.19", "GO:0005524", "GO:0005737", "GO:0006400", "GO:0032267", "KEGG:K04075", "SO:0001217", "UniRef:UniRef50_Q9PR68", "UniRef:UniRef90_Q9PR68" ], "nt": "ATGGCAATGTTAAATATGTACAAAAGAAATATTCGTGTTGTTTGTCATGTGAATTATCATAAACGCGAATCATCCGATCGTGATCAGCAAATAGTTGTCGATTTTTGTAGAAAAAATAATTTAATAATTGAAATTTTAGATGTTGATGAAAAAGTTTATGAACGTTATGGTCATATTGATAATTTTCAAACAAAAGCACGTTTAATTCGTTATGATTTTTTCAAAGAAATTGGTAAAAAATATAATATTGAAAATTTGTATATAGCGCATAATTTTGATGATTTTTTAGAAACGGCATATATGCAACGAGCTCGCCAATCAAAAGCCTTATTTTATGGCATTAAGGAAAGTAATGTTATTAATGGCATAATTGTTAAACGTCCGGTTTTATTTGTGCGTAAACAAACATTACAACGCTATTGTGATGAAAATAAAATTAAGTATGGTATTGACGAAACAAATGAATTAGATATATACGAACGCAATCGTGTTCGTAAAATAATTAGTTCTTGA", "aa": "MAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCRKNNLIIEILDVDEKVYERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVLFVRKQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISS", "aa_hexdigest": "811b6d1026d1acb9a28b1ea6b32a6b0d", "start_type": "ATG", "rbs_motif": "GGxGG", "genes": [ "tilS" ], "truncated": "both", "pseudogene": { "start": 93938, "stop": 94834, "observations": { "mutation": [ 94243, 94822 ], "stop": [ 94243, 94822 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR68", "query_cov": 1.0, "subject_cov": 0.5074626865671642, "identity": 0.971, "score": 335.0, "evalue": 8.91e-114, "gene_start": 1, "gene_end": 170, "reference_start": 28, "reference_end": 197, "reference_sequence": "MIKLWTNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCKKNNLIIEILNVDEKVYERYGHIDNFQAKARLIRYDFFKEIGKKYNIENLYTAHNFDDFLETAYMQRARQSKALFYGIRESNVINGIIVKRPVLFVRKQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISSWSSNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYKYDYFIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDDLISLISYNKNNNISNSNVDIIDDFNVIEKQVDNN" }, "paralog": false, "identity": 0.9697986577181208, "score": 584.0, "evalue": 1.6e-214, "description": "Internal stop codon at 94243, 94822. Nonsense mutation around 94243, 94822.", "psc": { "uniref90_id": "UniRef90_Q9PR68", "gene": "tilS", "product": "tRNA(Ile)-lysidine synthase", "ec_ids": [ "6.3.4.19" ], "uniref50_id": "UniRef50_Q9PR68", "cog_id": "COG0037", "cog_category": "J", "kegg_orthology_id": "K04075", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0032267", "GO:0006400" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR68", "EC:6.3.4.19", "UniRef:UniRef50_Q9PR68", "COG:COG0037", "COG:J", "KEGG:K04075", "GO:0005737", "GO:0005524", "GO:0032267", "GO:0006400" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR68" ], "product": "tRNA(Ile)-lysidine synthase" } }, "id": "GPKLONFDEH_203", "locus": "GCA000006625_00163" }, { "type": "cds", "sequence": "contig_1", "start": 94806, "stop": 95366, "strand": "-", "frame": 2, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "GO:0005737", "GO:0006412", "RefSeq:WP_006688439.1", "SO:0001217", "UniParc:UPI000013293A", "UniRef:UniRef100_Q9PR67", "UniRef:UniRef50_Q9PR67", "UniRef:UniRef90_Q9PR67" ], "nt": "GTGGAAAAATATTTAATTGTTGGATTAGGTAATCCAGGATCAAACTATGCAAAAACAAGACATAATGTTGGCTTTATGGTTATTAATGAGATTTGCAACAAACTTAATTTGTCTCTAGATAGTTCAAAATTTAATGGAATTTTTACTAAAACAATTTATAACAATTCCATAGTGTTTTTTTGTCAACCAACAACTTACATGAATTTAAGTGGTGAATTTGTTATCAAAATGCTTAATTTTTATAATATTCCAATTAAAAATTTAATAGTTATTTATGATGATGTTGATACGAAATTAGGAACGATAAAATTGAGAAAAAAGGGGTCCTCAGGAGGGCAAAATGGAATTAAAAATATTATTAACATTTTAAAAACAGATGAAATTAAACGCATTCGTATAGGAATTGATAAAGATCCACATATAAAATTAGATCAATATGTCTTATCGAATTTTAAGATTGACGAATTAGTTATAATTAAACCAGCTATTATAAAGGGCGCTTTAGCTACATTAGCAGCGATTGGTGAAGATTTTGATAAAGTTATGAACAAATTTAATTAA", "aa": "MEKYLIVGLGNPGSNYAKTRHNVGFMVINEICNKLNLSLDSSKFNGIFTKTIYNNSIVFFCQPTTYMNLSGEFVIKMLNFYNIPIKNLIVIYDDVDTKLGTIKLRKKGSSGGQNGIKNIINILKTDEIKRIRIGIDKDPHIKLDQYVLSNFKIDELVIIKPAIIKGALATLAAIGEDFDKVMNKFN", "aa_hexdigest": "cd970cf7be8030f43f0b2d0726761c61", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013293A", "ncbi_nrp_id": "WP_006688439.1", "uniref100_id": "UniRef100_Q9PR67", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013293A", "RefSeq:WP_006688439.1", "UniRef:UniRef100_Q9PR67" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR67", "uniref90_id": "UniRef90_Q9PR67" }, "psc": { "uniref90_id": "UniRef90_Q9PR67", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_Q9PR67", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045", "GO:0005737", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR67" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "GPKLONFDEH_204", "locus": "GCA000006625_00164" }, { "type": "cds", "sequence": "contig_1", "start": 95588, "stop": 96727, "strand": "+", "frame": 2, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "KEGG:K02338", "RefSeq:WP_006688589.1", "SO:0001217", "UniParc:UPI00000C1B8C", "UniRef:UniRef100_Q9PR66", "UniRef:UniRef50_B5ZAQ8", "UniRef:UniRef90_Q9PR66" ], "nt": "ATGGAAGTTTTTATTTCTATAAAAAAATTAATTGAAGCAATAAAATTTAGTACAACAATTGCTAATACAAACAATGCAAATGCTTTATTATTAGGTGTTTTGATTGAAGTTAGTCAAAATAAAATTAGTTTTAAGACGACAAATAATCAAGTGAGTGGTTATAAAGAAATTACTGAAGGATTTGAATATTTTAATAGTGGAAAAGTTTTAGTTACAGCTAAGATTATTTTAGGATTAATTTCTAAGCTTAAAGATAAATCTGTTTTATTAAAACAAATTGACACAAATATTTTATTAATTAAAACTGAAAATTTTGAAACTCAAATTAATACAATGAATATTGAAGGCTTTCCTAATTTAAACTTTAGTTTAGAAGATTACGTTAAAATCAGTTTGCCGCATCAAATTATGCAAGAGATTAATACAAAGGTTTTACCCAACGTTTTGAACAGTCAAGGAATTGAAAAAATTCAACCGATTTCTGGAATTTTGATTGATACTCAAACTTTAGAAAATAAGTTGATTGCTATTGGTACAGATAAAATTAAAGCTTCATGTTTAATAAGAGAATATATGGGTGAAAAATTTAAATTTATTGTCTCTTATTCAACAATGAAATTGATTATCGAAATCCTTAAAAATGTTGAATATAGTAATAATCAAACTGTTGATTTTTATGTTCGAAGTAGAAGTTTAATTTTTAAAATTAATGATGCAATTTTACAAACAAGAATGATTGATGGTGTTTATCCAAATATTTACTCAATTTTTGATGAAACAAATGAAGAAAATACATATGTTTTTGATCGTCGTTTATTGATAGAAATTATTGAACGGGGTATGAATATTGTTATGCAAGAACAAAACCCTAAAATTAGTATTAGAATAGACAAAAATGAAGCAGAAATCAGTTTAACAACTTTTGAAATTGGTAATATGAAAGAAAAAATGTCAATAGTTAATTTAAACAATAAAAGTGCTGAGTTTATTTTAAACCCTAGTTTGCTAGCTCATGTTTTAAAAAATTTTGATAACAACGATGTAACTTTTAAAGTTAAAGATGAAATTTTAAGACCTATTATTTTTATCGATACCAAAGATGTTGGATTTAAACAGATTTTATCAAGAATAAAAAATTAA", "aa": "MEVFISIKKLIEAIKFSTTIANTNNANALLLGVLIEVSQNKISFKTTNNQVSGYKEITEGFEYFNSGKVLVTAKIILGLISKLKDKSVLLKQIDTNILLIKTENFETQINTMNIEGFPNLNFSLEDYVKISLPHQIMQEINTKVLPNVLNSQGIEKIQPISGILIDTQTLENKLIAIGTDKIKASCLIREYMGEKFKFIVSYSTMKLIIEILKNVEYSNNQTVDFYVRSRSLIFKINDAILQTRMIDGVYPNIYSIFDETNEENTYVFDRRLLIEIIERGMNIVMQEQNPKISIRIDKNEAEISLTTFEIGNMKEKMSIVNLNNKSAEFILNPSLLAHVLKNFDNNDVTFKVKDEILRPIIFIDTKDVGFKQILSRIKN", "aa_hexdigest": "6ee0b32204ea492f46ce31320dd48e07", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1B8C", "ncbi_nrp_id": "WP_006688589.1", "uniref100_id": "UniRef100_Q9PR66", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B8C", "RefSeq:WP_006688589.1", "UniRef:UniRef100_Q9PR66" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR66", "uniref90_id": "UniRef90_Q9PR66" }, "psc": { "uniref90_id": "UniRef90_Q9PR66", "gene": "DnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_B5ZAQ8", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338" }, "pscc": { "uniref50_id": "UniRef50_B5ZAQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAQ8" ], "product": "DNA polymerase III, beta subunit" }, "genes": [ "dnaN" ], "id": "GPKLONFDEH_205", "locus": "GCA000006625_00165" }, { "type": "cds", "sequence": "contig_1", "start": 96979, "stop": 97083, "strand": "+", "frame": 1, "gene": "ybcJ", "product": "RNA-binding protein", "db_xrefs": [ "COG:COG2501", "COG:J", "KEGG:K14761", "SO:0001217", "UniRef:UniRef50_A0AAP9AC47", "UniRef:UniRef90_A0AAC9T049" ], "nt": "ATGGTTGATGAAAAAATAGAAAATAGACGTGGTCGTAAATTATATGATCAAATGATTATTAAAATAAAAAATCAATTGTATCAAATAGTTAAAACTAATGAGTAG", "aa": "MVDEKIENRRGRKLYDQMIIKIKNQLYQIVKTNE", "aa_hexdigest": "53bf703dacef26a493c3c118970f67a1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ybcJ" ], "truncated": "5-prime", "pseudogene": { "start": 96865, "stop": 97083, "observations": { "mutation": [ 96958 ], "stop": [ 96958 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T049", "query_cov": 1.0, "subject_cov": 0.4722222222222222, "identity": 1.0, "score": 67.4, "evalue": 4.51e-14, "gene_start": 1, "gene_end": 34, "reference_start": 39, "reference_end": 72, "reference_sequence": "MKKIFISTEYVTLNQFLKIAGLINNGGQAKFWLLENKVMVDEKIENRRGRKLYDQMIIKIKNQLYQIVKTNE" }, "paralog": false, "identity": 0.9861111111111112, "score": 139.0, "evalue": 4.55e-45, "description": "Internal stop codon at 96958. Nonsense mutation around 96958.", "psc": { "uniref90_id": "UniRef90_A0AAC9T049", "gene": "YbcJ", "product": "RNA-binding protein", "uniref50_id": "UniRef50_A0AAP9AC47", "cog_id": "COG2501", "cog_category": "J", "kegg_orthology_id": "K14761", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T049", "UniRef:UniRef50_A0AAP9AC47", "COG:COG2501", "COG:J", "KEGG:K14761" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AC47", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AC47" ], "product": "S4 domain-containing protein YaaA" } }, "id": "GPKLONFDEH_206", "locus": "GCA000006625_00166" }, { "type": "cds", "sequence": "contig_1", "start": 97095, "stop": 97229, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "nt": "ATGAACGATTCTAATAAAGAAAAAAAATACACCGCCGAAAGTATTAAAGTACTAGAAGGTTTAGAAGCAGTACGAAAACGTCCTGGTATGTACATTGGTTCAACCCAATCAGAGGGTCTACACCATATGATTTGA", "aa": "MNDSNKEKKYTAESIKVLEGLEAVRKRPGMYIGSTQSEGLHHMI", "aa_hexdigest": "a93637b41d64556bdddaeb83f0405c74", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A920FT50", "query_cov": 0.9090909090909091, "subject_cov": 0.8, "identity": 0.6, "score": 49.7, "evalue": 4.33e-07, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A920FT50" }, "pscc": { "uniref50_id": "UniRef50_A0A920FT50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "GPKLONFDEH_207", "locus": "GCA000006625_00167" }, { "type": "cds", "sequence": "contig_1", "start": 97260, "stop": 97505, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "nt": "ATGGGCGGATTTGCAACAATTGTTAGAGTTATTCTAAAAAAAGACGGATCAGTACGTGTAGAAGATGATGGACGCGGAATTCCAATTGAAATTCACGAAAAAACAGGCTTATCAGGTGTTGAAACAGTATTAACAGTTTTACATGCTGGTGGTAAGTTTGATAATGATAGTTATAAAGTGTCTGGTGGATTACATGGTGTTGGTGCCTCTGTTGTTAATGCTTTAAGTAAAAATTTTAAGGTTTGA", "aa": "MGGFATIVRVILKKDGSVRVEDDGRGIPIEIHEKTGLSGVETVLTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSKNFKV", "aa_hexdigest": "cd0671b3ab80aeb79da0d37027158844", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021B43CD8", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.568, "score": 99.4, "evalue": 9.24e-26, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A380EBM1" }, "pscc": { "uniref50_id": "UniRef50_A0A380EBM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "GPKLONFDEH_208", "locus": "GCA000006625_00168" }, { "type": "cds", "sequence": "contig_1", "start": 98271, "stop": 98885, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTGCTGAAGCACGACGACGTTCACATGAAGCACGTGAAGCTACACGGCGTAAATCACCTTTTGAATCAAATTCATTACCTGGTAAATTAGCTGACTGCTCAAATCGTGATTCAAGTGTTACTGAAATTTATATTGTCGAAGGAGATTCAGCGGGTGGTTCAGCAAAAACAGGTCGTGAGCGTGAATTTCAAGCAATTTTACCACTGCGTGGAAAGATTATTAATGTTGAAAAAGCTAAAATTGATAAGATTTTTGCTAATGAAGAAATTCAAAATATGATTACTGCTTTTGGAGCAGGAATAGGTCCTGAATTTAATATTGAAAAATTAAGATATTCAAAAATTATTATTATGACTGATGCGGATGTTGATGGCAGTCATATTCGAATTTTATTGCTAACATTTTTTTATCGATACATGTTGCCATTGATTCAAAATGGAAATGTTTATATAGCGCAACCACCATTGTATAAAGTAAGTTATGCTAAAACAATTAAATACGCATATTCTGATCAAGAGTTAGAAAGAATTAAATCAACAATATCAAATATTAAATATAACATTCAACGCTATAAAGGGTTAGGTGAAATGAATCCTGATCAATTGTGA", "aa": "MQAAEARRRSHEAREATRRKSPFESNSLPGKLADCSNRDSSVTEIYIVEGDSAGGSAKTGREREFQAILPLRGKIINVEKAKIDKIFANEEIQNMITAFGAGIGPEFNIEKLRYSKIIIMTDADVDGSHIRILLLTFFYRYMLPLIQNGNVYIAQPPLYKVSYAKTIKYAYSDQELERIKSTISNIKYNIQRYKGLGEMNPDQL", "aa_hexdigest": "414da027a88c3c33768eb728a9326c3c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028ACB8D2", "query_cov": 0.9509803921568627, "subject_cov": 0.9326923076923077, "identity": 0.593, "score": 224.0, "evalue": 2.94e-71, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_A0A7L7RZ60" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23147.202100000013, "isoelectric_point": 9.175349998474118 }, "id": "GPKLONFDEH_209", "locus": "GCA000006625_00169" }, { "type": "cds", "sequence": "contig_1", "start": 98895, "stop": 99047, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AWV8" ], "nt": "ATGGATCCTAAAAATAGACTTTTATTAAAAGTTAATATTGAAGACGCTGCAATTGCTGATAAAACATTTTCTCTATTAATGGGTGATGATGTAGCTCCAAGAAAAGAATTTATTGAAAAAAATGCAAAATATGTAAAAAATATTGATGCTTAA", "aa": "MDPKNRLLLKVNIEDAAIADKTFSLLMGDDVAPRKEFIEKNAKYVKNIDA", "aa_hexdigest": "4d2f0a52842685f0712bbbc265b4eff8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A538DNN0", "query_cov": 0.98, "subject_cov": 0.8166666666666667, "identity": 0.633, "score": 67.8, "evalue": 4.74e-14, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A2H6AWV8" }, "pscc": { "uniref50_id": "UniRef50_A0A2H6AWV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AWV8" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "GPKLONFDEH_210", "locus": "GCA000006625_00170" }, { "type": "cds", "sequence": "contig_1", "start": 99069, "stop": 99326, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase type IIA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A960F5Y9" ], "nt": "ATGGCATTAAAAAAACCAAAAAAAAGTCGTTTAACCACCGAAGAAATTAAGCAACAATTAGAAGGTTCAACGATTAAAGAACAATCAATTACAAAAGAAGTCGAAACATCATTTTTAGATTATTCAATGTCTGTAATCGTTGCACGTGCTTTGCCTGATGTGCGTGATGGATTTAAGCCCGTGCATCGCCGAGCTTTATTTGCTGCTTTCGAAAACGGAATGACACATGATAAGCCTTATAAAAAATCTGCACGTTGA", "aa": "MALKKPKKSRLTTEEIKQQLEGSTIKEQSITKEVETSFLDYSMSVIVARALPDVRDGFKPVHRRALFAAFENGMTHDKPYKKSAR", "aa_hexdigest": "7d697c8fafb414dda9228e5a0310968d", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A920J4M1", "query_cov": 0.8941176470588236, "subject_cov": 0.8539325842696629, "identity": 0.5, "score": 69.3, "evalue": 9.82e-14, "valid": false, "product": "DNA topoisomerase type IIA domain-containing protein", "uniref50_id": "UniRef50_A0A960F5Y9" }, "pscc": { "uniref50_id": "UniRef50_A0A960F5Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A960F5Y9" ], "product": "DNA topoisomerase type IIA domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_211", "locus": "GCA000006625_00171" }, { "type": "cds", "sequence": "contig_1", "start": 99393, "stop": 101591, "strand": "+", "frame": 3, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335", "KEGG:K02469", "SO:0001217", "UniRef:UniRef50_P47719", "UniRef:UniRef90_Q9PR63" ], "nt": "ATGGCACAAGACTTTTCAATGCGTTATTTATTAGTTGATGGTCATGGTAATTTTGGTTCGATTGATGGTGATAGTGCAGCTGCGATGCGTTATACAGAAGCACGATTATCAAAAATCTCTTATGAATTATTAAAATACATTGATAAAGAAACAGTAGATTTTGTACCTAATTATGATGCATCAGAACAAGAACCAAGTGTTTTACCATCAGGTTTTCCAAATTTATTAACAAATGGAACTACAGGAATAGCTGTTGGGATGGCAACAAATATTCCACCACATAATTTAACTGAAGTTTGTCAAGCAATAAAAGCTTATGCTAAAAACCATAATGTTACTATTTCTGAAATTATGGAATATCTAAAAGGTCCTGATTTTCCAACAGGCGCTGAAATTTATGGTGATAGCGGTATTATTAAATATTTTAATACAGGTCGTGGTTCAGTAACAATTCGTTCAAAATATGAAATTGAAGATATTGGGCAAGGACGTGTAGCAATTGTTGTGACAGAAATTCCTTATATGGTTAATAAAGCTAATTTAATTGAAAAAATTGTTGAATTAGTAACAAACAAACAAATCGAAGGAATTTCTGATTTACGTGACGAGTCAAGTCGTGATGGAATTCGAATTGTTATTGAAGTTAAACGTGATGTTATACCTGAAGTTTTATTAAACAAATTATTTAAAACAACACCTTTACAAACAAATTTTAGTGTTAATAATTTAGCTTTAGTTAATGGTGTACCAATGGTATTAAACATTAAGGAAATGATTAAATACTATTTTGAACACCAAATTGAGATTTTAGTAAGAAGAACAAATTTTGATTTAAAAAAAGCAAAAGAACGAATTCATATTGTTGAAGGTTTAGTAATTGCTGTTAATAATATCGATGAAGTAATTAAAATTATTAAAGCCTCAGGTGATGATGATATTGCTTCAAAATCACTAATTCAACGTTTCGATTTAACAGAATTGCAAACGAAAGCAATTTTAGAAATGCGTTTACGCGCTTTAACAGGACTAAATATTGATAAATTAAAAAAAGAATATGAAGATTTAATATTAGTTATTAAAGATTTAGAAGATGTTTTAAACAACTACAATCGTCAAGTTAATATTATTTGTGAAAATTTAGATTATTTAATTGAAAAATTTGGTGATGAGCGTCGTACAGAAATTATGTATGGAGTTAGTTCGCATATTGATGATGAAGATTTAATACCAGTCGAAGATATTGTTGTAACAATGTCAAAACGTGGTTATTTTAAACGTTTGCCAATTGATACATATAAAAATCAACGACGTGGTGGTGTAGGTGTACAAGGATTAAAAACGTATGAGGATGATGATGTTGAAAAAATCTTAGTTGCTAATACCCATACAGATTTATTATTCTTTTCTGATTTAGGTCGTGTTTATCGATTACGAGGACATGAAGTACCATTGGGATCACGACAATCAAAAGGAATTCCAGCAATTAATTTTTTACCAATTGAAAAAAGTGAAAGCATTTTAACTATTTTACCAATAGATAATTATGATCAAGGTTCATTATTCTTTACAACAAGTAAAGGAATTATAAAACGAGCTAATCTAAGTGATTTCGAGTCAATTCGTGCTAATGGTAAAATTGCTATTACTTTAAAAGATGGTGATAAATTATTTTCAGTAATGCAAACACTAGGTAATGATGAAGTATTTATCGGCGCATCAAATGGTAATGTAATTCGTTTCAATGAAAATGATGCACGTGAAATGGGAAGAATTGCGACAGGTGTTAAAGGAATTAATTTAGAAAATGATGAATATGTTGTAGGAACAGGGTTATCTAGTCATGGAGAATATGTTTTAGCTGTTGGTTCTAAAGGATTAGGTAAATTAACTGACATCAATGATTATCGTTTAACTAAGCGTGGAGCAAAAGGTGTTAATACACTAAAAGTAAATGATAAAACAGGTAACTTAGTAAGTATTAAAGTTGTTAATCGTGAAGAAGAAGCTTTAATTATTACAACTAGTGGAAAAGTTATTCGTTTATCGATTAAAGACATTAGTGTAATTGGAAGAAATACATCAGGAGTTAAATTAATTTCATTAGAAAATAAAGAAGAAGTAAAATCAATTGCTATCTTTAAAAAAGAAGAAATTAATGATGAACAATTATTACAAGAAAATGATTACAATCTAAAATAA", "aa": "MAQDFSMRYLLVDGHGNFGSIDGDSAAAMRYTEARLSKISYELLKYIDKETVDFVPNYDASEQEPSVLPSGFPNLLTNGTTGIAVGMATNIPPHNLTEVCQAIKAYAKNHNVTISEIMEYLKGPDFPTGAEIYGDSGIIKYFNTGRGSVTIRSKYEIEDIGQGRVAIVVTEIPYMVNKANLIEKIVELVTNKQIEGISDLRDESSRDGIRIVIEVKRDVIPEVLLNKLFKTTPLQTNFSVNNLALVNGVPMVLNIKEMIKYYFEHQIEILVRRTNFDLKKAKERIHIVEGLVIAVNNIDEVIKIIKASGDDDIASKSLIQRFDLTELQTKAILEMRLRALTGLNIDKLKKEYEDLILVIKDLEDVLNNYNRQVNIICENLDYLIEKFGDERRTEIMYGVSSHIDDEDLIPVEDIVVTMSKRGYFKRLPIDTYKNQRRGGVGVQGLKTYEDDDVEKILVANTHTDLLFFSDLGRVYRLRGHEVPLGSRQSKGIPAINFLPIEKSESILTILPIDNYDQGSLFFTTSKGIIKRANLSDFESIRANGKIAITLKDGDKLFSVMQTLGNDEVFIGASNGNVIRFNENDAREMGRIATGVKGINLENDEYVVGTGLSSHGEYVLAVGSKGLGKLTDINDYRLTKRGAKGVNTLKVNDKTGNLVSIKVVNREEEALIITTSGKVIRLSIKDISVIGRNTSGVKLISLENKEEVKSIAIFKKEEINDEQLLQENDYNLK", "aa_hexdigest": "ddb475e8438ace3b9658b83d13a196b0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR63", "query_cov": 1.0, "subject_cov": 0.8611764705882353, "identity": 1.0, "score": 1401.0, "evalue": 0.0, "valid": true, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47719", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47719", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47719" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "GPKLONFDEH_212", "locus": "GCA000006625_00172" }, { "type": "cds", "sequence": "contig_1", "start": 101699, "stop": 102130, "strand": "+", "frame": 2, "gene": "pncC", "product": "CinA family protein", "db_xrefs": [ "COG:COG1546", "COG:H", "EC:3.5.1.42", "KEGG:K03743", "SO:0001217", "UniRef:UniRef50_A0A084EWD4", "UniRef:UniRef90_A0AAC9T390" ], "nt": "ATGAATGTGGCTTTAGAACTAATTAATTTACTAAAAGAAAGACGTTTAAGATTGAGTGTTTGCGAATCAGCAAGTTGTGGAGCTTTATCATCATCAATTGGTGAAGTTGCAGGTGCTTCAAGTGTTTTTGCAGGTGGTTTTATTAGTTATAGTAATGAAGTAAAAATTAAATTAGTAGGAGTGAGTGAAAAAACAATTTTTAAATATGGCGCTGTTTCTGAACAAACTGCTAAAGAAATGTGTTTACAAACAAATCAAAAGTTTAATACTGATATTGCAATTTCAATTACAGGAAATGCTGGACCACAGGGAAGCGAAAATAAAGAAGTAGGTTTATTTTACATTGGAATCGCAATTAAAGATTTTGCAATTGTAAAAAAAGTCACATTAAATTCAAATGAACGTACTTTTAATCGTTTTTCTATAGCATGA", "aa": "MNVALELINLLKERRLRLSVCESASCGALSSSIGEVAGASSVFAGGFISYSNEVKIKLVGVSEKTIFKYGAVSEQTAKEMCLQTNQKFNTDIAISITGNAGPQGSENKEVGLFYIGIAIKDFAIVKKVTLNSNERTFNRFSIA", "aa_hexdigest": "b2c46351efc1f6738d0c87beeb5b142d", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0AAC9T390", "query_cov": 1.0, "subject_cov": 0.9166666666666666, "identity": 0.937, "score": 253.0, "evalue": 1.3e-84, "valid": true, "gene": "PncC", "product": "CinA family protein", "ec_ids": [ "3.5.1.42" ], "uniref50_id": "UniRef50_A0A084EWD4", "cog_id": "COG1546", "cog_category": "H", "kegg_orthology_id": "K03743" }, "pscc": { "uniref50_id": "UniRef50_A0A084EWD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EWD4" ], "product": "Competence-damage inducible protein" }, "genes": [ "cinA", "pncC" ], "id": "GPKLONFDEH_213", "locus": "GCA000006625_00173" }, { "type": "cds", "sequence": "contig_1", "start": 102208, "stop": 103074, "strand": "+", "frame": 1, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "COG:COG0468", "COG:L", "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664", "KEGG:K03553", "SO:0001217", "UniRef:UniRef50_Q9PR61", "UniRef:UniRef90_Q9PR61" ], "nt": "ATGAAAGAAAATAATAATCTTGAGAAATTAGATCCAATTGCAACACTTGAAACAAAATTTGCTAAATCATCTTATTTTATTGCTGATGAAATAAAAGATGAAAAAATTAATGCAATATCTACAGGATCAATTCATATAGATCAAATAACAGGAATTAATGGAATTCCAGTAGGAAAAATTACAGAAATTTATGGTAATGAATCATCAGGTAAAACTACAATTGCTTTACAAACAATTGCTGAATGTCAAAAAACTGGTGGGACAGTAGTTTTGTTAGATCTTGAGGGCTCATTTGATATTAATTATGCCAAAAGTCTAAAAGTCGATTTAACAAAATTAATCATTACGCAACCTCAAACAGGTGAACAGGCTTTTGATATGATTGAAACATTGATTAAAACAAATTCAATAGATTTAATAGTTATTGATTCAGTTGCTGCAATGCTCCCAGAAAGCGAATATCAAGCAAATATGAATGAAGCATTGATGGGAGCACACGCTCGCTTAATGTCAAAAGGTTTACGTAAAATTCAACCATTAATTAATAAAAGTAAAACAGCCATTATTTTTATTAATCAATTACGTGAAAAAATTAATACTTTTTTTGGCAATCCTGAAATGACCACAGGCGGTAAAGCTTTAAAATTTTATGCTAGTTTGAGAATAGAAACCAAAAAAGCAGATTTAATTAAAGAGGGTATTAATAAAATTGGTATAAAAACAAAAGTAACAACAGTTAAAAACAAATTAGCACCACCGTTACAAACATGTTTTATTGATATTTTTTTTGGTTCTGGTTTCGATTATAATAATGAAATTATAGATTTTGCAATCCAATATGGCGTCTTGAAAAAAAACGGTTCTTGA", "aa": "MKENNNLEKLDPIATLETKFAKSSYFIADEIKDEKINAISTGSIHIDQITGINGIPVGKITEIYGNESSGKTTIALQTIAECQKTGGTVVLLDLEGSFDINYAKSLKVDLTKLIITQPQTGEQAFDMIETLIKTNSIDLIVIDSVAAMLPESEYQANMNEALMGAHARLMSKGLRKIQPLINKSKTAIIFINQLREKINTFFGNPEMTTGGKALKFYASLRIETKKADLIKEGINKIGIKTKVTTVKNKLAPPLQTCFIDIFFGSGFDYNNEIIDFAIQYGVLKKNGS", "aa_hexdigest": "5ed91fc98f26812405d289bf97114e77", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PR61", "query_cov": 1.0, "subject_cov": 0.8622754491017964, "identity": 1.0, "score": 556.0, "evalue": 2.13e-199, "valid": true, "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_Q9PR61", "cog_id": "COG0468", "cog_category": "L", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR61" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "GPKLONFDEH_214", "locus": "GCA000006625_00174" }, { "type": "cds", "sequence": "contig_1", "start": 103244, "stop": 104665, "strand": "+", "frame": 2, "gene": "rny", "product": "Ribonuclease Y", "db_xrefs": [ "COG:COG1418", "COG:JR", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0005886", "GO:0006402", "RefSeq:WP_006688749.1", "SO:0001217", "UniParc:UPI0000139226", "UniRef:UniRef100_Q9PR60", "UniRef:UniRef50_Q9PR60", "UniRef:UniRef90_Q9PR60" ], "nt": "ATGGGTTATTTAATAGCATTTATTATATTACTAATATTATTTGTATTGCTGATTACCATTGTTCCAGTTGTAATGGTAGTTTATCTTAAGAAAAAACAACTGAAATTAACTTTTGTTCCAAAAAGCCAAACTTCTTTTAAAAAAATAGTTCAAAAAACTAAAGATTTAGAAGAAGAATGTGAAGATTTAAATAATAAAAATAATGAATTAAAAAAAGCAATTAGTGATCAAAATTTACAAATTGATTTACTCAAAAAAAACAATGAAAATTTTTTACTAAATGCCACAAGTTTAACAGCTGAACAAGCGAAAAAAGAACTTTTTAATTTATTAAAAATAAAATTTAAAAAAGAGCTTGCTCAAGAATATGCAAAAATTAAACACGAATTTAATGAAGCCCAAGAAATTTATGCTCAAAACATTCTAGTAGAAACAATGGAGCAAATTGCTGAGCCTTTAATTGTTGAAAGATCTTTATTTAATATTGACATTATTGATGAAAATCTAAAAGGAAAAATTATTGGACGTGATGGACGTAATAAAGCAGTTTTTGAAAATGAAGGCGGAGTTGATCTAATTGTTGATCGACAACAGCCAATTGTAGGAATATCTACTCCAAATCCAATTCGTCGTGAAATAGCACGAATTGTTATGCAAAAATTAATTGATTCAAAAAATATTGATATTAATCGTATTGAATTACTTTTTAAAGAAGAACGTGAAAAGTTTGAAAAAAAAGTTTTTGAGATTGGTAAAAACGTCGTAGAACAAACATTAGGTTTTTTTGATTTACCTGAAGGAATTTATTCTTATATAGGGAGAATGAAATTTCGTAATTCTTATGGTCAAAATATTTTATCCCATTCATTAGAAGTTGCTGAATATGCTGAACGAATTGCTAAATTAATTAATATTGATCCTATAAAGGCAAAAAAAGCTGCATTTTTTCATGATATAGGAAAAACGATTGATTTTGAAAGTGACCTAGATCATGTAGAAGCGGGATTATTGATTGCTAAGAAATTTAATTTAGATGATTATATTTATAATGCAATTGAATCACATCATAACAAAGTAATTCCAACAACAATTTATGGTGCATTAGTTAAAATAGTTGATACGCTATCAGCAGCCAGACCAGGAGCACGTGTTAATTCTTATGATGAATATTACAGTCGTGTAAAAGAACTTGAAACAATATGTATGCGATTCGAAGGTGTTAAAAGCGCCTATGTCATTAAATCTGGTCGTCAATTACGTGTAATTGTTGATTCTAACTTGGTAAGTGATGAGCAATTAGAACTCTTAGGTCACGAGATCAAAGTTGCAATTGAAGAAAATGATTTATTAACAAATTATAAAATTAAGATCGTTTTAATTAAAGAAAAAAGGATTTCAATTGACACAAATATTATTGGATAG", "aa": "MGYLIAFIILLILFVLLITIVPVVMVVYLKKKQLKLTFVPKSQTSFKKIVQKTKDLEEECEDLNNKNNELKKAISDQNLQIDLLKKNNENFLLNATSLTAEQAKKELFNLLKIKFKKELAQEYAKIKHEFNEAQEIYAQNILVETMEQIAEPLIVERSLFNIDIIDENLKGKIIGRDGRNKAVFENEGGVDLIVDRQQPIVGISTPNPIRREIARIVMQKLIDSKNIDINRIELLFKEEREKFEKKVFEIGKNVVEQTLGFFDLPEGIYSYIGRMKFRNSYGQNILSHSLEVAEYAERIAKLINIDPIKAKKAAFFHDIGKTIDFESDLDHVEAGLLIAKKFNLDDYIYNAIESHHNKVIPTTIYGALVKIVDTLSAARPGARVNSYDEYYSRVKELETICMRFEGVKSAYVIKSGRQLRVIVDSNLVSDEQLELLGHEIKVAIEENDLLTNYKIKIVLIKEKRISIDTNIIG", "aa_hexdigest": "a5b48f3042b4600528c12e293211b979", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139226", "ncbi_nrp_id": "WP_006688749.1", "uniref100_id": "UniRef100_Q9PR60", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139226", "RefSeq:WP_006688749.1", "UniRef:UniRef100_Q9PR60" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR60", "uniref90_id": "UniRef90_Q9PR60" }, "psc": { "uniref90_id": "UniRef90_Q9PR60", "gene": "rny", "product": "Ribonuclease Y", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_Q9PR60", "cog_id": "COG1418", "cog_category": "JR", "go_ids": [ "GO:0005886", "GO:0003723", "GO:0004521", "GO:0006402" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR60" ], "product": "Ribonuclease Y" }, "genes": [ "rny" ], "id": "GPKLONFDEH_215", "locus": "GCA000006625_00175" }, { "type": "cds", "sequence": "contig_1", "start": 104864, "stop": 105004, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACAAAAATTATATGGTTAATGTTTGTAAAGATTTTGGTATTAACTATGTTGTTTTAGATGCAAACAATTCTGTTGAACAAAACGTTGATGAAGTTACAAAAATATTACAAAAGTTAGAAAATGAGCAAGACAAATAA", "aa": "MYKNYMVNVCKDFGINYVVLDANNSVEQNVDEVTKILQKLENEQDK", "aa_hexdigest": "b4fb615d9d1f8c70e7e037b31f12aefc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5397.0319, "isoelectric_point": 4.507070732116699 }, "id": "GPKLONFDEH_216", "locus": "GCA000006625_00176" }, { "type": "cds", "sequence": "contig_1", "start": 105230, "stop": 105562, "strand": "-", "frame": 3, "gene": "dck", "product": "Deoxyguanosine kinase", "db_xrefs": [ "COG:COG1428", "COG:F", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_Q9PR59" ], "nt": "ATGTCAGATAAAAAATTAGATTTTTTAACACCATCTTTTAAAAAAAATAGGGTTTCTAATTCTATTGCAATAGGTGGTATGATTGCTTTTGGTAAATCTACTTTAGCACAAGCTTTACACGAAAAATATCAACCATCAAATGTGGTTTATGAAATGGTTGAGGGTGATAAATTAATGGATTTATTACTAGCAAAAATGTATGAACGTGAGAATAACGTTCTTTTTGGGTCTTTATTTCAACTATATTTTGTTTTAAATCGTTTTGCAAACTACAAAAATAATTGTAACCGTAAAAGTTTAACAATTTTTGATCGTAGTATTTTTGAAGATTGA", "aa": "MSDKKLDFLTPSFKKNRVSNSIAIGGMIAFGKSTLAQALHEKYQPSNVVYEMVEGDKLMDLLLAKMYERENNVLFGSLFQLYFVLNRFANYKNNCNRKSLTIFDRSIFED", "aa_hexdigest": "b0a50e25ef785d87d35cdeeaf072912e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "dck" ], "truncated": "5-prime", "pseudogene": { "start": 104927, "stop": 105562, "observations": { "mutation": [ 105100, 105172, 105232 ], "stop": [ 105100, 105172, 105232 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR59", "query_cov": 1.0, "subject_cov": 0.47413793103448276, "identity": 0.973, "score": 217.0, "evalue": 5.97e-70, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MSDKKLDFLTPSFKKNRISNSIAIGGMIAFGKSTLAQALHEKYQPSNVVYEMVEGDKLMDLLLAKMYERENNVLFGSLFQLYFVLNRFANYKNNCNRENLTIFDRSIFEDWLFAHANIDKPSVFSYYDGLWQGVCKELIYEHGVPKLYVILDGDWELFKERIFKRNRKVEIDNFSINETYFKRLLEMYKNYMVNVCKDFGIDYIVLDARNSVEHNVNEVTKRLQELKNEQNK" }, "paralog": false, "identity": 0.957345971563981, "score": 409.0, "evalue": 6.93e-148, "description": "Internal stop codon at 105100, 105172, 105232. Nonsense mutation around 105100, 105172, 105232.", "psc": { "uniref90_id": "UniRef90_Q9PR59", "gene": "Dck", "product": "Deoxyguanosine kinase", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR59", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "GPKLONFDEH_217", "locus": "GCA000006625_00177" }, { "type": "cds", "sequence": "contig_1", "start": 105760, "stop": 106167, "strand": "+", "frame": 1, "gene": null, "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A353KRR9" ], "nt": "ATGAGCGAATCAACATTGTACCGTAAATATCGGCCACATAAATTTAAAAATGTAATAGGTCAAGATTTAATTAAACAAGCATTAATGAATGCTTGTTTAAACAACAAAATTACACATGCTTATATTTTTAGTGGACCACGAGGAATTGGAAAAACATCAATAGCTAGAATTTTTGCGTCTAGTATTAATTGTTTAAATCCTAATAAAGGTGATTGTTGCGGCAAATGTAGTGTTTGTGAAAGTATTCTCAAAGAACAAAGTGTTGATTTGATTGAATTAGATGCTGCTAGTAATAATGGTGTTGATCAAATACGTATTATTACTGATAATATAAATTATTTACCAACATTTTTTAAGTATAAAGTCTATATTATTGATGAAGCACATATGCTATCTACATCAGCATGA", "aa": "MSESTLYRKYRPHKFKNVIGQDLIKQALMNACLNNKITHAYIFSGPRGIGKTSIARIFASSINCLNPNKGDCCGKCSVCESILKEQSVDLIELDAASNNGVDQIRIITDNINYLPTFFKYKVYIIDEAHMLSTSA", "aa_hexdigest": "e56702bb6b382239b5095571ed3a3469", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003266BD28", "query_cov": 1.0, "subject_cov": 0.8653846153846154, "identity": 0.5479999999999999, "score": 153.0, "evalue": 2.53e-45, "valid": false, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A353KRR9", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A353KRR9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A353KRR9" ], "product": "DNA polymerase III subunit gamma/tau" }, "genes": [], "id": "GPKLONFDEH_218", "locus": "GCA000006625_00178" }, { "type": "cds", "sequence": "contig_1", "start": 106546, "stop": 107553, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTGAATTTATTTATGAATTATACAATCAAGGTGTTAATTTAGAAGCTTTTTGTGTGCAAATAATAAATGTTTTAATTGATTATTTAATTTATTTAAAAACAAACGATATAAATAATCTTAAAAAAGTATCAGTTGATGAATTAAAAAAAATGTTATTAATTAATTTTAATGGTAATTATTTGTTAAATAATTTTATTTCTTTATTTAATAATTTAAAAAAATCATTAAATCAAGTTTTTGAATTTGAAATTTATTTATATAAAATAATCAATGCTAATAATGATAAATTAGAAAATAAAAAAATAGAATTGTCAATTAACCCTGAAGAAAATAAAATTAATAATTCCCAAATAATAACGACAAAAAAAATCGATTGTTTTGAAGAAATAAATAAAAATACTGTTGATTTTGATAAGTTATACAAAACAAAAATTTTTTATCACAAAAAAATTAACAACCAGAATGATAAAGAATCAGGTATTAATCAAAAAGAAGAATTAAGTACAGAATTAGTTAATGATAATAAATTGATTTGCAATTCTAATGATATATTAGATAATTATGAACTAGCCAAACAAGCTTTTTTTAATAAAGACATTAAACTTTCAAAAGAAATGAGTCAAAAATTTAAAGATTTTAAAAATGAAGAAACCATTGAAAATAGTTATATTGATATTATTAAACAAGCTGATTTAGTACTTTGGTGCTCACCTAATGCACTAGTTTTAGGAGTAGAGTTTTTAGGATTAATTAATCGTATCAATAAAGTTACGCATTCATTTGAATTTATTAAAGAATTTGTTAAGAAATTTGATTCTATCAAGTTAGTTATTGCTATTAGCAAAAAACAAGCATTAAATATTATTAATATTAAAAAACAAGGTTTAAAAACTAATATAATTAATGATGTTTTAATTGATGATATTAAAATGTTATTAAAACAAGAAGAACAACGAAAACAAGAACAAACAGCGTTATTAAATGAAATTGAAGATGAGGAGTAA", "aa": "MREFIYELYNQGVNLEAFCVQIINVLIDYLIYLKTNDINNLKKVSVDELKKMLLINFNGNYLLNNFISLFNNLKKSLNQVFEFEIYLYKIINANNDKLENKKIELSINPEENKINNSQIITTKKIDCFEEINKNTVDFDKLYKTKIFYHKKINNQNDKESGINQKEELSTELVNDNKLICNSNDILDNYELAKQAFFNKDIKLSKEMSQKFKDFKNEETIENSYIDIIKQADLVLWCSPNALVLGVEFLGLINRINKVTHSFEFIKEFVKKFDSIKLVIAISKKQALNIINIKKQGLKTNIINDVLIDDIKMLLKQEEQRKQEQTALLNEIEDEE", "aa_hexdigest": "2d4aee1af9a8564f23f47c8734d675ea", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 39394.95030000001, "isoelectric_point": 5.613271141052246 }, "id": "GPKLONFDEH_219", "locus": "GCA000006625_00179" }, { "type": "cds", "sequence": "contig_1", "start": 107561, "stop": 107860, "strand": "+", "frame": 2, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "GO:0005737", "GO:0043590", "RefSeq:WP_006689002.1", "SO:0001217", "UniParc:UPI0000139231", "UniRef:UniRef100_Q9PR57", "UniRef:UniRef50_Q9PR57", "UniRef:UniRef90_Q9PR57" ], "nt": "ATGGACTTTCAAAAACTTGCACAAGAATTAAAAAAAATGCAAAACACTTTAAGTAAAAAACAAAAAGAATTTGAAGAAAAAGTGTTTGATTTTGATTATAAAGGATATGTACTTGTTAAAATCAAAGGTGATTTAAATATTGAAGCAATTGAAATAAAAACAGAAATAGTGGATCCTGAAGACAAAGAAACATTACAAGATATTTTACGTGCAGCGATCAATGAAGCAATCTCTATAACATGTAAAGAACGAGATGCGATTATGAATGCTACAATCCCTAAAGGAACAGGTCTTTTTTAG", "aa": "MDFQKLAQELKKMQNTLSKKQKEFEEKVFDFDYKGYVLVKIKGDLNIEAIEIKTEIVDPEDKETLQDILRAAINEAISITCKERDAIMNATIPKGTGLF", "aa_hexdigest": "6e82fb463ef7243e080604f93ad8379c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000139231", "ncbi_nrp_id": "WP_006689002.1", "uniref100_id": "UniRef100_Q9PR57", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139231", "RefSeq:WP_006689002.1", "UniRef:UniRef100_Q9PR57" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR57", "uniref90_id": "UniRef90_Q9PR57" }, "psc": { "uniref90_id": "UniRef90_Q9PR57", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_Q9PR57", "cog_id": "COG0718", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0043590" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR57" ], "product": "Nucleoid-associated protein UU088" }, "genes": [ "ybaB" ], "id": "GPKLONFDEH_220", "locus": "GCA000006625_00180" }, { "type": "cds", "sequence": "contig_1", "start": 107962, "stop": 108474, "strand": "+", "frame": 1, "gene": "recR", "product": "recombination mediator RecR", "db_xrefs": [ "COG:COG0353", "COG:L", "GO:0003677", "GO:0006281", "GO:0006310", "GO:0046872", "KEGG:K06187", "SO:0001217", "UniRef:UniRef50_Q9PR56", "UniRef:UniRef90_Q9PR56" ], "nt": "TTGTTACAACAAGATCAAAAATACATTGATGATCTTATTAAAAGAATTAAAGAAGCAAAAACAAATATTTTAAAATGTAAGTATTGTGCTAATTTTACAAATAAAGACGAATGCGATATTTGTTTAAATGAATATCGTGATTTTACTAAATTAATGATTGTTACCACAAACGAGGATTTAGAAAGAATCGAATCAGCTAACATTTATAATGGCCTATATCATATTACAAATGGTGAAATCTCTTTAAGGAAAAACGTTGTTATTGAACATACAAACATTAAGACTATTAAAGAGCGTGTTTTAAATGGTAGTTTTAAAGAAATAATTATTGCTACAAGTTATACCCATGATGGGGAAGTTACGGCTGATTATATTATAAGAATGCTTGAAGACATTAAAGATTTACAAATTTATCGTATTGGCTTTGGTATTCCTTTAAATTCAAGCATTGATTATGCAGATGATGAAACTTTAAAGCAGTCTCTTATTAATAAACGGAAAATAAGAAATTAA", "aa": "MLQQDQKYIDDLIKRIKEAKTNILKCKYCANFTNKDECDICLNEYRDFTKLMIVTTNEDLERIESANIYNGLYHITNGEISLRKNVVIEHTNIKTIKERVLNGSFKEIIIATSYTHDGEVTADYIIRMLEDIKDLQIYRIGFGIPLNSSIDYADDETLKQSLINKRKIRN", "aa_hexdigest": "be7f0a4e8a2f9291ed1aa12cd520bb74", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PR56", "query_cov": 1.0, "subject_cov": 0.845771144278607, "identity": 0.9940000000000001, "score": 337.0, "evalue": 7.61e-117, "valid": true, "gene": "recR", "product": "recombination mediator RecR", "uniref50_id": "UniRef50_Q9PR56", "cog_id": "COG0353", "cog_category": "L", "kegg_orthology_id": "K06187", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR56" ], "product": "Recombination protein RecR" }, "genes": [ "recR" ], "id": "GPKLONFDEH_221", "locus": "GCA000006625_00181" }, { "type": "cds", "sequence": "contig_1", "start": 108556, "stop": 108798, "strand": "+", "frame": 1, "gene": null, "product": "putative protein UU089-1", "db_xrefs": [ "RefSeq:WP_006689098.1", "SO:0001217", "UniParc:UPI000165F8D3", "UniRef:UniRef100_Q9PR55", "UniRef:UniRef50_Q9PR55", "UniRef:UniRef90_Q9PR55" ], "nt": "ATGGGTTACGGTAAAGGATACTTAGCAATGTTTAAAAACAAGAAAGTAAGATTTAAGGTTGTAAATAGCTTTCCTGATCTAAAGGTTCAATTTGTTACATCATTTCCTGATTACAAAGTAAAGATTTCAAATTCATCTTCTTTTTGTGAGGAAACTATTAAAATTCAAGTTGTTACTAGCTTTCCTGATGTAAAATTACAAAAAGTTACATCATTCGGTGATTTTGAAGCTTATATTGACTAA", "aa": "MGYGKGYLAMFKNKKVRFKVVNSFPDLKVQFVTSFPDYKVKISNSSSFCEETIKIQVVTSFPDVKLQKVTSFGDFEAYID", "aa_hexdigest": "42915c0bfe137614476cf48b2b7c9021", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000165F8D3", "ncbi_nrp_id": "WP_006689098.1", "uniref100_id": "UniRef100_Q9PR55", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8D3", "RefSeq:WP_006689098.1", "UniRef:UniRef100_Q9PR55" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR55", "uniref90_id": "UniRef90_Q9PR55" }, "psc": { "uniref90_id": "UniRef90_Q9PR55", "product": "Uncharacterized protein UU089.1", "uniref50_id": "UniRef50_Q9PR55" }, "pscc": { "uniref50_id": "UniRef50_Q9PR55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR55" ], "product": "Uncharacterized protein UU089.1" }, "genes": [], "id": "GPKLONFDEH_222", "locus": "GCA000006625_00182" }, { "type": "cds", "sequence": "contig_1", "start": 108895, "stop": 110598, "strand": "+", "frame": 1, "gene": "gmrSD", "product": "GmrSD restriction endonucleases N-terminal domain-containing protein", "db_xrefs": [ "COG:COG1479", "COG:V", "RefSeq:WP_006689055.1", "SO:0001217", "UniParc:UPI000165F8D4", "UniRef:UniRef100_A0A2C9DXZ3", "UniRef:UniRef50_A0A2C9DXZ3", "UniRef:UniRef90_A0A2C9DXZ3" ], "nt": "ATGGCTAATGAGTATTATAATATGTGTGCCTTAGATGCACTCAAATTTGTGAAAAATAGAAATATGTTATTACCAGATATTCAAAGAGAATATGTTTGGGATACAACTGATATAGAAAAATTATTTGAATCTATAGTTGATGATTATCCAGTGGGTTCTTGTATATTTTGGAAAACAAATAAAAAAGTACTTAATGATGAAAAACCAAATCTTTATTATTTTATTTCTAAATATAAGCAAGGCGTAACTAAAAATGAAAAAGCACCAGAATTTGTATCTGATGAAACAGATTATTATATAGTATTAGATGGACAACAAAGAATTACATCTCTTAATATTGCATTGTTTGGATCGTTTACATCTAAGAAAAAAGGTAAAGGATATAGTGTAAAAAATTCTAAATCATGGGTAGAACGAGAACTATATTATAATTTATCTTTTTACGACAGCAATGAATTGGATGATGAAAATCCCATTAAAAGATTTGTATTTTTGTCTGAAGAAGAAGCAAAGAATGGTAATTATTATAAAGTTAAGCAAATAGTTAAAAGTGATAACTTAACTGATTATGTTGATACAATATCGAATTTTAATAAAAATGTAAAAACGGATTTAATGTGTTTGTATAGAAGAATTATGGAAGCGTCTGCCACTAGTGTAATTCATTATTATTGCATTGCAGAGAATACATATGATGAAGCACTAGATATTTTTGTTAGAGTAAATTCTACTGGAAGAAAACTATCAAAGTCCGATTTATTATTTTCTACATTAATTGATGGTTGGAAAGAAGGGAAGGAAAATATAGACACCTTATTGTCTGACTTAAATTCAGCTGGAGATGGATTTTCTTTTACAAGAGATTATTTAATGCGATTGGCTTTGGTATTAGTTGATGCAAATACTAATTTAAAAATTGATTCGTTAACTTCTAAAACAATAAAGGAAATCAGGGATAACTGGCAAAAAATAAAGAAAGCGCTAGAAAAATTATCTGAATTATTAGTTTCAATTGGCATGTGTGACGAAAATATGACCTCATATAATGCAACCATTCCACTGGCATATTATTTTTACAAAGGTGGCAAAATTGATACAGCAGAATCAAAAAAAGAAGCAAGAAAGTTCCTAGCCGTTTCAATGTCAAAAGGACTGTTTGGTGTAGCAAGTAATGATTCTTTGAATCAAAGCAGAACTGCTCTTAAGTCAGTAAATGAAAAAACTCCATTTTCATTAACTTTATTTAGTAGTATTACTTTAACGGGTAATAGAACATTTACTGCATCGAAAGATGATATTGAATATTGGTTGACGAAGTTTGAAAAAGGAAAAAACACATTCATAATTCTTTCTTTGCTTTATCCTAATTTAAAGTTAAGCCAAGTTGAGTTCCATCAGGATCACTGTCACCCTTATACTTCATTTGATGATAAAAACATTAAAAAATTAGGATTGACAGAAGATAAAATAAAAGAATGGCAGTTTAAAAGAAATTTAATTCCTAATTTGCAGTTTTTGGCAGGTAAGGAAAATGAGTCAAAAAATAAAAGGCCTTTATCAAATTGGGTTAAAGATGGAAATACCTTTGAATATAGACCAGAAAATGTTTCTCTTGAATTATCCAATTTTGATAAATTTTTTAATGAAAGAAAATCCCTTATAAAAAATAAGCTTTTGGATATTTTTGGTTTAAAAGCAAACTAA", "aa": "MANEYYNMCALDALKFVKNRNMLLPDIQREYVWDTTDIEKLFESIVDDYPVGSCIFWKTNKKVLNDEKPNLYYFISKYKQGVTKNEKAPEFVSDETDYYIVLDGQQRITSLNIALFGSFTSKKKGKGYSVKNSKSWVERELYYNLSFYDSNELDDENPIKRFVFLSEEEAKNGNYYKVKQIVKSDNLTDYVDTISNFNKNVKTDLMCLYRRIMEASATSVIHYYCIAENTYDEALDIFVRVNSTGRKLSKSDLLFSTLIDGWKEGKENIDTLLSDLNSAGDGFSFTRDYLMRLALVLVDANTNLKIDSLTSKTIKEIRDNWQKIKKALEKLSELLVSIGMCDENMTSYNATIPLAYYFYKGGKIDTAESKKEARKFLAVSMSKGLFGVASNDSLNQSRTALKSVNEKTPFSLTLFSSITLTGNRTFTASKDDIEYWLTKFEKGKNTFIILSLLYPNLKLSQVEFHQDHCHPYTSFDDKNIKKLGLTEDKIKEWQFKRNLIPNLQFLAGKENESKNKRPLSNWVKDGNTFEYRPENVSLELSNFDKFFNERKSLIKNKLLDIFGLKAN", "aa_hexdigest": "ebfcf6bf2850e03a0ca78e3264fd60cd", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI000165F8D4", "ncbi_nrp_id": "WP_006689055.1", "uniref100_id": "UniRef100_A0A2C9DXZ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8D4", "RefSeq:WP_006689055.1", "UniRef:UniRef100_A0A2C9DXZ3" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DXZ3", "uniref90_id": "UniRef90_A0A2C9DXZ3" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DXZ3", "gene": "GmrSD", "product": "GmrSD restriction endonucleases N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DXZ3", "cog_id": "COG1479", "cog_category": "V" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DXZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DXZ3" ], "product": "GmrSD restriction endonucleases N-terminal domain-containing protein" }, "genes": [ "gmrSD" ], "id": "GPKLONFDEH_223", "locus": "GCA000006625_00183" }, { "type": "cds", "sequence": "contig_1", "start": 111214, "stop": 111693, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28", "PFAM:PF02881.24" ], "nt": "ATGTTCAAAGCAATGATAGGTAGTATTGTAAGTAAACAAATGTCAAAAAAATTAAAAAATGCGACAATTGTTGAAGAAGATATTAAAGAATTATTAAGCGAGATAAGAATAGCATTATTAGATGCTGACGTTAATTTATTAGTTGTTAAAAAATTTATTAAAAACATCAAAGAAAAAACAATTGGATTGTATGTTGAAGCAAACCAAAAGCCAGCAGATCTTGTTTTAAAAATAATTAAAGATGAATTAGTTGAAATTTTAGGAAAAGAAAATAAGCCAGTTAACACAGCTAAATCACAATTAAAAATTATGATGGTTGGTTTACAAGGTTCAGGTAAAACAACAACTACTGGTAAATTAGCTAATTATTTTAAAAATAAATACAATAAAAAACCATTATTAGTGGCGGCTGATATCTATCGTCCAGCGGCGATTGATCAATTAAGAACATTAGCAAAACAAGTTCGTGTAGATTTTTGA", "aa": "MFKAMIGSIVSKQMSKKLKNATIVEEDIKELLSEIRIALLDADVNLLVVKKFIKNIKEKTIGLYVEANQKPADLVLKIIKDELVEILGKENKPVNTAKSQLKIMMVGLQGSGKTTTTGKLANYFKNKYNKKPLLVAADIYRPAAIDQLRTLAKQVRVDF", "aa_hexdigest": "36bf195b4a9ec7a68a9eefb09c558ecb", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI00267073CB", "query_cov": 0.9119496855345912, "subject_cov": 0.9931506849315068, "identity": 0.517, "score": 142.0, "evalue": 1.15e-40, "valid": false, "product": "signal recognition particle receptor subunit alpha", "uniref50_id": "UniRef50_A0A533RPK5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 57, "aa_cov": 0.3584905660377358, "hmm_cov": 0.9824561403508771, "evalue": 3.728217915176334e-24, "score": 81.0204086303711, "start": 102, "stop": 158 }, { "id": "PF02881.24", "name": "SRP54_N", "length": 74, "aa_cov": 0.44025157232704404, "hmm_cov": 0.9594594594594594, "evalue": 2.060064309617424e-14, "score": 49.1660270690918, "start": 14, "stop": 83 } ], "seq_stats": { "molecular_weight": 17802.046399999992, "isoelectric_point": 9.784705162048336 }, "id": "GPKLONFDEH_224", "locus": "GCA000006625_00184" }, { "type": "cds", "sequence": "contig_1", "start": 111766, "stop": 111927, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28" ], "nt": "TTGATAATTGTTGATACAGCTGGACGTTTACAAACAAACGAAGAGTTAATGCAAGAATTAGTAAATGTTAAAAAAACACTAAATCCTGATGAGGTTTTTTTAGTTGTTGATGCTATGGCAGGCCAAGACATTATTAACGTTGCTACAGAATTCAATAATTGA", "aa": "MIIVDTAGRLQTNEELMQELVNVKKTLNPDEVFLVVDAMAGQDIINVATEFNN", "aa_hexdigest": "aa6d8e26e94eef48d252fe29ddcbe451", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 53, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.928705817701044e-18, "score": 62.37493896484375, "start": 1, "stop": 53 } ], "seq_stats": { "molecular_weight": 5905.684400000002, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_225", "locus": "GCA000006625_00185" }, { "type": "cds", "sequence": "contig_1", "start": 112078, "stop": 112332, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGGCTGATCGAATTTTAGGTCTTGGTGATATCATGACTTTAGCAGAGAAAGCAGCTGATGTTATTGATGAAAAACAAGTTCGTGGTTCGATGCAACGAATGATGGCAGGAAAAATGGATTTAGAAGATTTAATGCGTCAAATGAGTCAAATTAGTAAATTAGGTAGTTTTTCAGGAATAGCTAAGATGATTCCTGGATTGAACAATATTTCTGAAAACCAAATTGGTGATGCTGAGAGCAAAATGAAAATATGA", "aa": "MADRILGLGDIMTLAEKAADVIDEKQVRGSMQRMMAGKMDLEDLMRQMSQISKLGSFSGIAKMIPGLNNISENQIGDAESKMKI", "aa_hexdigest": "c4c9e2694e695a49e21b0ed97258ab73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 44, "aa_cov": 0.5238095238095238, "hmm_cov": 0.8181818181818182, "evalue": 4.477644116821719e-09, "score": 32.236419677734375, "start": 38, "stop": 81 } ], "seq_stats": { "molecular_weight": 9203.733099999994, "isoelectric_point": 5.295029640197753 }, "id": "GPKLONFDEH_226", "locus": "GCA000006625_00186" }, { "type": "cds", "sequence": "contig_1", "start": 112351, "stop": 112560, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGACTTTAAAAGAACGTCGTGATCCGCGCGTTTTTAAAAAAGAACCATCAAGACGCATACGGGTTTTAAAAGGATCAGGACGTTCGCCTGATGAATTAAATAAGTTATTAAAACAGTGGGAAGTGTCACGTGATAAAATGGCAGAACTTGGTAAAATGTTACAAAAAGGAAAAAATCCTTTTAGTAAATCTGGTGGGTTATTTAGTTAA", "aa": "MTLKERRDPRVFKKEPSRRIRVLKGSGRSPDELNKLLKQWEVSRDKMAELGKMLQKGKNPFSKSGGLFS", "aa_hexdigest": "b5bba1a0fe10a3b040f4d97952383c8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 47, "aa_cov": 0.6811594202898551, "hmm_cov": 0.9574468085106383, "evalue": 9.539280122912643e-12, "score": 40.79910659790039, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 8002.314199999999, "isoelectric_point": 10.807560539245607 }, "id": "GPKLONFDEH_227", "locus": "GCA000006625_00187" }, { "type": "cds", "sequence": "contig_1", "start": 112772, "stop": 113089, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAATAAATTTGAACAGCAGTTTAGCTTATATTGGTTTTTTAACACGCCTTATTATTGTAAAAAAAACAACATATCTTTTACCGATCATAACTTTATTAATTACATTAATTATAGGAATTGTTGTCGGCTGTGTTGTTAAGCAAAAAACACAATTTTTGATAATTTCATATGTAATGATCATGTTTAATTTATTAATGACAACAATTTTTGCTTCACTAAAAGCATTAAATATTTTTAAGGATTTTAGTGAAGATGGTATTGATATTTTAGTAATTTCTAAACCAATTAGCCGTAAAAACATTGTTTGA", "aa": "MNKKINLNSSLAYIGFLTRLIIVKKTTYLLPIITLLITLIIGIVVGCVVKQKTQFLIISYVMIMFNLLMTTIFASLKALNIFKDFSEDGIDILVISKPISRKNIV", "aa_hexdigest": "399f6e14d227f586fc6402c241144580", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11835.454799999983, "isoelectric_point": 9.944586753845215 }, "id": "GPKLONFDEH_228", "locus": "GCA000006625_00188" }, { "type": "cds", "sequence": "contig_1", "start": 113927, "stop": 114070, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTAAAGAGGTATTAAACGTAAGATCGTTTAATACATATGCTAATAATTTATTTAAAACACTTGATCAAAAAGCAAATCAAAAACAAATCTTAGATTTAATTTCAAATAGTGTACAAAAATTTGATTTCAATAAATGA", "aa": "MIIKEVLNVRSFNTYANNLFKTLDQKANQKQILDLISNSVQKFDFNK", "aa_hexdigest": "1d383e58e8c52a2facdb2461f6e69aea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5533.317300000001, "isoelectric_point": 9.601614952087402 }, "id": "GPKLONFDEH_229", "locus": "GCA000006625_00189" }, { "type": "cds", "sequence": "contig_1", "start": 114323, "stop": 114493, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTCAAGAAAATGACGATGGCATAGATGAATTAAAAAAAGATAAAAAGATTCGTTACAGATATAGTTTAGAACAAGGCGGAAATTATTTATTCGATTATGTCCAACAAAATTATAGTTTTAAAAGAAAGCAACAAATTGTTTATAAAGAAGCTTACTGCGTAATTTGA", "aa": "MPQENDDGIDELKKDKKIRYRYSLEQGGNYLFDYVQQNYSFKRKQQIVYKEAYCVI", "aa_hexdigest": "090e91dad0a8b2322d8f047d492a4950", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6860.651200000002, "isoelectric_point": 7.787152290344237 }, "id": "GPKLONFDEH_230", "locus": "GCA000006625_00190" }, { "type": "cds", "sequence": "contig_1", "start": 114559, "stop": 115269, "strand": "+", "frame": 1, "gene": "rbbA", "product": "ABC transporter", "db_xrefs": [ "COG:COG1131", "COG:JV", "RefSeq:WP_006688981.1", "SO:0001217", "UniParc:UPI00000C1B95", "UniRef:UniRef100_Q9PR51", "UniRef:UniRef50_A0A449B6T2", "UniRef:UniRef90_Q9PR51" ], "nt": "ATGAATAATGAATTTATTTTAGAAGTTAAAAATTTAACAAAAATTTATAAAAAATCAAACCAAGGGGTTTTTGATTTAAGTTTTAATGTAAAACGTGGCGAAATTCATGCATTTATTGGTGAAAATGGATCAGGTAAAACAACAACTATTAAATCAATTATTAATGCATACACAAATTACAAAGGATCAATTTTAATTGATGGTTTTGATAATAAAACCCCAAAAAGTCATGAAAAAATTGGTTATGTGCCAGAAATTGCTTTATTTCCTAGTGAATTAACAACTTTTCAATATTTATATTCATTTGCTCGTTTATCAAATTTAAATAAAAAACAAGCTATTGAACGCATTCATAATTTTTTAGAAAAATTTAGAATTAGTGATTTAAAAAATAAAAAACCAATCAATTTTTCATCTGGACAAAAAAAGAAAGTGATTTTAATTCAAGCTTTATTACACGATCCAGAGTTAATTATTTTAGATGAACCAACAGCGAACTTAGACCCATCAGCACGTCATGAATTCAATAATATCTTAAAAGAATTGCACGAACAAAACAAAACCATTTTTATTTGTTCGCATATTTTAAAAGAAATGGATACTTACGTTGATAGTTTGACTTTAATTCACAAAGGAAAGCTAGTATATAGTGGTCATAAATATGATGCATTAGAGAAAATCTACTATGACAGTGTTTTAAAAAATAAATAA", "aa": "MNNEFILEVKNLTKIYKKSNQGVFDLSFNVKRGEIHAFIGENGSGKTTTIKSIINAYTNYKGSILIDGFDNKTPKSHEKIGYVPEIALFPSELTTFQYLYSFARLSNLNKKQAIERIHNFLEKFRISDLKNKKPINFSSGQKKKVILIQALLHDPELIILDEPTANLDPSARHEFNNILKELHEQNKTIFICSHILKEMDTYVDSLTLIHKGKLVYSGHKYDALEKIYYDSVLKNK", "aa_hexdigest": "891831325da65e8af51cec3ba8b67ace", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00000C1B95", "ncbi_nrp_id": "WP_006688981.1", "uniref100_id": "UniRef100_Q9PR51", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1B95", "RefSeq:WP_006688981.1", "UniRef:UniRef100_Q9PR51" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR51", "uniref90_id": "UniRef90_Q9PR51" }, "psc": { "uniref90_id": "UniRef90_Q9PR51", "gene": "RbbA", "product": "ABC transporter", "uniref50_id": "UniRef50_A0A449B6T2", "cog_id": "COG1131", "cog_category": "JV" }, "pscc": { "uniref50_id": "UniRef50_A0A449B6T2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B6T2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "rbbA" ], "id": "GPKLONFDEH_231", "locus": "GCA000006625_00191" }, { "type": "cds", "sequence": "contig_1", "start": 116276, "stop": 116842, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTATTATTTTAATAATATTCGTGTTGAAAAACAAGTTACAAAGGTTAAAAACAATGCTAAACGTGGAGACCAAAACGCAGAAGAAAATTTTCCAAAAGAATTTTATTTCGGTGCTAATAGTAATGAAAAATTTAGCTTTGGTACACACTCATACACATTAAATAATGAAATCACACAAGCTTCATATGAACAAGATATTTTTAGTTTAGATCATAAAATTAAAAACGAATTAAAAAAATTAAATGAACAACAAATTTTAAAAGATATTGAATATGCATTTACTAGTGGTTATGATGTAGCAATTGATTCCATTGGTAGTATTTTTAACATTTTAAAAGGTGTTGCTAATGATTTAAATTTAAAAGAATTGTTTTTACAATCATCACAAGATTTTAGAAATTTAACTTATAACATTACACAACAGAATAATTTGACTAATTTAATCACTTTAATTACATCAAATCAATCATTAGGGATGGTTATTGATGGTTTTAAACCAATTTTAAAAGAAATTATTTATAATAATCCAAGTATTGACCAATCAGTTAAAAATACAATTTGA", "aa": "MRYYFNNIRVEKQVTKVKNNAKRGDQNAEENFPKEFYFGANSNEKFSFGTHSYTLNNEITQASYEQDIFSLDHKIKNELKKLNEQQILKDIEYAFTSGYDVAIDSIGSIFNILKGVANDLNLKELFLQSSQDFRNLTYNITQQNNLTNLITLITSNQSLGMVIDGFKPILKEIIYNNPSIDQSVKNTI", "aa_hexdigest": "a38f052e60d0680413b63bf82b6a4563", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21672.044100000006, "isoelectric_point": 5.742693519592286 }, "id": "GPKLONFDEH_232", "locus": "GCA000006625_00192" }, { "type": "cds", "sequence": "contig_1", "start": 116858, "stop": 117397, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTAAAAATAATTTAATTGCTGAAATTGGACAAATTAGAACCTTACTTGATAGTATCTCATTAACTGAAATTAAAACCTATAAACCAATTATTAATAAATTATTAGAATTAATTTCTTTAATTGAAGCACGTTCTAAAAAAGACCCTAAAAATTATGGATTTATTGATGGTTTGGACGAACTATTTTCCTTCTTTTTAAATTTAAAACAAAATGATCTACCAAGTAGTATTAATTTTAAAATTGGTGATCAAAATTATACTTTATATGATTTAATTACTGAAGTTAAAAAAATTGCAAATAACTTAATTCCGCATGAAAACCAATATGACCCTAAAGACCAACAAATTATCAGTACCAATTATTTTTTAACAAAAATTAAAATTTTAGATTTGATTTCTTTAAACAAAAATGACCAACTTGATTATCATAAGGGTGTTGAAATGATTTTTAGTTTATTAAAAAAATTTCAAATAGCAATTCCCGAATTTATTTATAAAATTATTGATGAATTACTAATTCAAAATACTAATTGA", "aa": "MDFKNNLIAEIGQIRTLLDSISLTEIKTYKPIINKLLELISLIEARSKKDPKNYGFIDGLDELFSFFLNLKQNDLPSSINFKIGDQNYTLYDLITEVKKIANNLIPHENQYDPKDQQIISTNYFLTKIKILDLISLNKNDQLDYHKGVEMIFSLLKKFQIAIPEFIYKIIDELLIQNTN", "aa_hexdigest": "96dc02a91b31174daf4290bc0a40b743", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20874.94330000001, "isoelectric_point": 5.443436241149903 }, "id": "GPKLONFDEH_233", "locus": "GCA000006625_00193" }, { "type": "cds", "sequence": "contig_1", "start": 117860, "stop": 117970, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAATTCTATGAAAGCAATCATTGAAAATATATCATTAAGAAACGATCGTTTATTACCTAATAAATATGTTGCTCAATTATTAAATTGGTTATTTTATAAAAAATGA", "aa": "MPNSMKAIIENISLRNDRLLPNKYVAQLLNWLFYKK", "aa_hexdigest": "69a95f493ae9f351e3c18006d6a1318b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4336.131800000001, "isoelectric_point": 9.994614219665529 }, "id": "GPKLONFDEH_234", "locus": "GCA000006625_00194" }, { "type": "cds", "sequence": "contig_1", "start": 118310, "stop": 118555, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGGATTTGGGAAAAAAGTTGATTTAAAAAAAGATGTTTATTTGAGTGCAGTTGCATTTAATTTTGGTTTAAACACTAACGATAGTGATCAAACAAACGTCTTAAATAATAATGATGAAATTAAATACAATCTAAATATTTTAAAAAAAGTAGTTATCACATTACACTTTACTAAGCCAATGTTAGATTTAAGTGATCCTAATCGCACTAAATTAGTTAATTCTTATACTTTTGTTGTTTAG", "aa": "MLGFGKKVDLKKDVYLSAVAFNFGLNTNDSDQTNVLNNNDEIKYNLNILKKVVITLHFTKPMLDLSDPNRTKLVNSYTFVV", "aa_hexdigest": "527f2c73e05b71538cb4f10a29f25569", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9209.513299999993, "isoelectric_point": 8.96376476287842 }, "id": "GPKLONFDEH_235", "locus": "GCA000006625_00195" }, { "type": "cds", "sequence": "contig_1", "start": 118702, "stop": 118830, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688744.1", "SO:0001217", "UniParc:UPI000165F8D5", "UniRef:UniRef100_A0A2C9DZ08", "UniRef:UniRef50_A0A2C9DZ08", "UniRef:UniRef90_A0A2C9DZ08" ], "nt": "ATGATCATTAATTTCCAAATGCTAATAGATTTATATAAAAATTATATTGGAAAAGAAAATATTCAATTACCAAGTAATTTTTCAAAAGAAGATATTGTGATGAAAAAAATTCAAAAAGTAGTTAAGTAA", "aa": "MIINFQMLIDLYKNYIGKENIQLPSNFSKEDIVMKKIQKVVK", "aa_hexdigest": "80f705ee5e85c02c2bf22264adca1632", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI000165F8D5", "ncbi_nrp_id": "WP_006688744.1", "uniref100_id": "UniRef100_A0A2C9DZ08", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8D5", "RefSeq:WP_006688744.1", "UniRef:UniRef100_A0A2C9DZ08" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DZ08", "uniref90_id": "UniRef90_A0A2C9DZ08" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DZ08", "uniref50_id": "UniRef50_A0A2C9DZ08" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DZ08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DZ08" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5015.994300000002, "isoelectric_point": 9.45688343048096 }, "id": "GPKLONFDEH_236", "locus": "GCA000006625_00196" }, { "type": "cds", "sequence": "contig_1", "start": 118942, "stop": 119091, "strand": "-", "frame": 1, "gene": null, "product": "Conserved domain protein", "db_xrefs": [ "RefSeq:WP_012316997.1", "SO:0001217", "UniParc:UPI000165F8D6", "UniRef:UniRef100_A0A2C9DYI3", "UniRef:UniRef50_A0A2C9DYI3", "UniRef:UniRef90_A0A2C9DYI3" ], "nt": "GTGATTAATGATATTGCTGATTCTTTTGATTATAAAAATATATATGCAATAAAAATTTCAAATTTAATAGTTTTAACTCATAATTTTGATTTTTATAGAACAATATCATTGGGATTTGATGTTTGCAAAGAATCGCAATTTGTTGTATAA", "aa": "MINDIADSFDYKNIYAIKISNLIVLTHNFDFYRTISLGFDVCKESQFVV", "aa_hexdigest": "9aa218bb9e77aed41c5e89c6aa0e9626", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8D6", "ncbi_nrp_id": "WP_012316997.1", "uniref100_id": "UniRef100_A0A2C9DYI3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8D6", "RefSeq:WP_012316997.1", "UniRef:UniRef100_A0A2C9DYI3" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYI3", "uniref90_id": "UniRef90_A0A2C9DYI3" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DYI3", "product": "Conserved domain protein", "uniref50_id": "UniRef50_A0A2C9DYI3" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYI3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYI3" ], "product": "Conserved domain protein" }, "genes": [], "id": "GPKLONFDEH_237", "locus": "GCA000006625_00197" }, { "type": "cds", "sequence": "contig_1", "start": 120158, "stop": 121141, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12008.13", "PFAM:PF22679.2" ], "nt": "TTGGACAAAAAACTAAAATATCATAGTTTAATTCAAGCAATGTCGCGAACCAATCGAATTCTTAATGGTGTTAAATCAAATGGTAATATTGTCTTTTTCATAAATTTAGAAGATGAAGTGAATGAAGCTTTAAGATTATTTTCATCAGAGAGCAATATTAATTCTTCGATCGCTATTTTAAGATCATTTAAAGAATATTATGAAGGATATTATAATGAAGATAATCAATATATACAAGGTTATCGAAACTTAGTAGAAGAATTAAAAAGTAACTTTAAAATTCCTTTTAATGGAACAAAAGAAGAGGAAAGAATTTTTATTAAACTTTTTGGTGATATTTTAAAATATAAAAATTTGCTTTCACAATTTAGTGAATTTGAACAAAACGATGATCTTATTGGTACAGAAATGCAAGATTATCAAAGTATATATATCGGGTTGCGTGATAAATATCAAGATAAAAAACGTATGGAAACAGATAGCATTGTTAGTGATCTTGATTTTAAAATTGAATTAATTAGTCAACAAGATATTGGTGTTGATTATATTCTTAAGTTAATGAATGAAAAGCTCAAAAAAGATCTAAAAGAAGTTAAATATCACGATCTAGAAAGAGATATTTTATCTAGTCTAAAAGCGCGAAAAAAGAAAGATTTAATTATTGAATTTGTTAATGAAATTAATAAACTACCTAATAAGGATGAAATTGATATTTTTAATGAATGGAGTAAGTTTATTAAAAATAAAAAAGAAAGTGAGCTTATAAAAATAATTAATGAAAATAAATTAGATAACAAAAAAACATTTGAATTTATGAAAAAAGCATTTGAAAATAATGAATTTAATGAAAATGGAGTAGATATTGTTAATTTAATGCCTCCTACCTCTTTTTTTAAAAAAAGTAATCAGAATAGTAATAGGTCTAAAATAAAAGAAAATGCTACTGAAAAATTACTCGAATATTTTGAAAAATTCCGTGGTTAA", "aa": "MDKKLKYHSLIQAMSRTNRILNGVKSNGNIVFFINLEDEVNEALRLFSSESNINSSIAILRSFKEYYEGYYNEDNQYIQGYRNLVEELKSNFKIPFNGTKEEERIFIKLFGDILKYKNLLSQFSEFEQNDDLIGTEMQDYQSIYIGLRDKYQDKKRMETDSIVSDLDFKIELISQQDIGVDYILKLMNEKLKKDLKEVKYHDLERDILSSLKARKKKDLIIEFVNEINKLPNKDEIDIFNEWSKFIKNKKESELIKIINENKLDNKKTFEFMKKAFENNEFNENGVDIVNLMPPTSFFKKSNQNSNRSKIKENATEKLLEYFEKFRG", "aa_hexdigest": "f491262bea6f1eda73abd6cafc59e46a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12008.13", "name": "EcoR124_C", "length": 275, "aa_cov": 0.8134556574923547, "hmm_cov": 0.9381818181818182, "evalue": 2.8307744934855087e-51, "score": 170.16136169433594, "start": 60, "stop": 325 }, { "id": "PF22679.2", "name": "T1R_D3-like", "length": 33, "aa_cov": 0.10091743119266056, "hmm_cov": 1.0, "evalue": 2.870694751730882e-12, "score": 42.88441848754883, "start": 1, "stop": 33 } ], "seq_stats": { "molecular_weight": 38890.7951, "isoelectric_point": 6.0218297958374025 }, "id": "GPKLONFDEH_238", "locus": "GCA000006625_00198" }, { "type": "cds", "sequence": "contig_1", "start": 121142, "stop": 122266, "strand": "-", "frame": 3, "gene": null, "product": "DEAD/DEAH box helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3E2CM26" ], "nt": "GTGGATAAGGTTCTATTTGTTGTTGATCGAAAAGATTTAGACTATCAAACAACAAAAGAATATGAAAAGTATTCTAAAGGATGTGTTAGTGCTAATACAAATGTTAATGAACTAGCTAAGCAAATTGGAAATCAAGAGAATAAAATTATTATTACAACCATTCAAAAACTTGGCATATTCATTAGTAAAAATCCAAAACATCCTATTTATTCTAAAAATGTTGTTCTTGTTTTTGATGAATGTCATCGTTCTCAATTTGGTGATTTACATAAAAAAATTACTAAAAAATCTTTTAAAAAATATATGATTTTTGGGTTTACAGGCACACCTATTTTAGAAGAAAATAGTAATGTCAAAAATAAAAATATTTTCTTTACAACAGAGCATCTTTTTGGAGCTTGTTTACATAAATATACAATTGATAAAGCTATTCGTGATGGTAATGTTTTAGGTTTTAGAACGGAGTATTATAAAACACTTAGCGAAAAAGATGACATTGACGATGAAACATCATCAACTGGAATAGATAAAAAAGAAGCTTTGGAAAATCCGATTCGTATTTCTGAAATTGTTGATTTTATTTTAAAAAACTATAATCGTTTAACCAAAAGAAATGAAGAATTTTTTGAACATAATAAGATTGCTAATATAAATGAAGTTATTACTAGCAGAAATAAATTAAGCGAAAATAAAGTATTAACAACACTAAATGGATTTAATTCTATTCTAGCTTGCTCTTCTATTAATGCAGCTAAAATATATTATTCAGAATTTAAAAAACAACAACAAAAATTTCCTCAAGAAGAAAAATTAAAAATTGGATTAATTTATAGTTATTTACCAAGCGAACAAGAAAGTAATGATAATGAACTTTCTAATTTTGAACTATTAGATGAGAATAACGAAAACACTAATCAACTTGATTTAGATTCACGTAATTTTCTTGATGAAGCGATTAAAGATTATAATGAAATGTTTGGGACATCATACTCAACAGATAATGCTAATTTTAATAATTACTATAAAGACTTTAGCTTGAGATTAAAGGATCGAAGCTTAGATATAGCAATCGTTGTTAATATGTTTTTAACAGGTTTTGATGCAACAACTTTAAATACATTATGA", "aa": "MDKVLFVVDRKDLDYQTTKEYEKYSKGCVSANTNVNELAKQIGNQENKIIITTIQKLGIFISKNPKHPIYSKNVVLVFDECHRSQFGDLHKKITKKSFKKYMIFGFTGTPILEENSNVKNKNIFFTTEHLFGACLHKYTIDKAIRDGNVLGFRTEYYKTLSEKDDIDDETSSTGIDKKEALENPIRISEIVDFILKNYNRLTKRNEEFFEHNKIANINEVITSRNKLSENKVLTTLNGFNSILACSSINAAKIYYSEFKKQQQKFPQEEKLKIGLIYSYLPSEQESNDNELSNFELLDENNENTNQLDLDSRNFLDEAIKDYNEMFGTSYSTDNANFNNYYKDFSLRLKDRSLDIAIVVNMFLTGFDATTLNTL", "aa_hexdigest": "d7573903a16ea6c5373601cdd9c22180", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030771B9D", "query_cov": 1.0, "subject_cov": 0.9280397022332506, "identity": 0.511, "score": 345.0, "evalue": 1.15e-113, "valid": false, "product": "HsdR family type I site-specific deoxyribonuclease", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A3E2CM26", "kegg_orthology_id": "K01153" }, "pscc": { "uniref50_id": "UniRef50_A0A3E2CM26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3E2CM26" ], "product": "DEAD/DEAH box helicase" }, "genes": [], "id": "GPKLONFDEH_239", "locus": "GCA000006625_00199" }, { "type": "cds", "sequence": "contig_1", "start": 122336, "stop": 122449, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTAAGACCATACCAAATTGTTGCAACTGAAAGAATTATTAATCGAATTAAGATTTCTTTAAATGATAAAAAATTATTAGGAACTACAGGCGCTGGTGGTTATATTTGA", "aa": "MVLRPYQIVATERIINRIKISLNDKKLLGTTGAGGYI", "aa_hexdigest": "c881ed184ac004cb6bbf9f1f3280a982", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4116.870700000001, "isoelectric_point": 10.160878181457516 }, "id": "GPKLONFDEH_240", "locus": "GCA000006625_00200" }, { "type": "cds", "sequence": "contig_1", "start": 122570, "stop": 122833, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTATTTTGATTAATGGACTTCCATTAGTACATATTGAACTAAAAAGACGAGGAACTAATCTTAAAGAAGCTTTTAATCAAATTAAAAGATATGCAAATCATTCGTTTACAGATTTATATCTTTACACGCAAATTTATGTTATTTCTAATGGTACACAAACTAAATATTATTCAAATACAACTAGAGAAACTTCTATAAAAGAAAGAAATAATACAAACTCTAAAAAAACAAGTAATTCTTTTGAATTTACATCATACTGA", "aa": "MSILINGLPLVHIELKRRGTNLKEAFNQIKRYANHSFTDLYLYTQIYVISNGTQTKYYSNTTRETSIKERNNTNSKKTSNSFEFTSY", "aa_hexdigest": "2b2b8d68e2ab7e349de56445efc26a7c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10207.334999999992, "isoelectric_point": 9.7413179397583 }, "id": "GPKLONFDEH_241", "locus": "GCA000006625_00201" }, { "type": "cds", "sequence": "contig_1", "start": 123056, "stop": 123295, "strand": "-", "frame": 3, "gene": null, "product": "type I restriction endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307E52CF", "UniRef:UniRef90_UPI00307E52CF" ], "nt": "ATGGAAAATAAGGTACTATTAGCAGAACATGAAAATTCAACGGTTGTTGCTGAATTTAAATCAATTAAAGTTCATTCGAATAAATTTGAAAGTGAAGCTGAATTAGAAGAAACATTTATTAAATTATTAATTGCACAAGGTTATGAATACTTACCTATTAAAAATAATAATGATTTAAAAAATAATTTAAGAAAACAAATCGAAAAACTTAATAATTATCAATTTACTAATAATGAATGA", "aa": "MENKVLLAEHENSTVVAEFKSIKVHSNKFESEAELEETFIKLLIAQGYEYLPIKNNNDLKNNLRKQIEKLNNYQFTNNE", "aa_hexdigest": "50aa796711ea9af7342e30f457eb02ef", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 122990, "stop": 123295, "observations": { "mutation": [ 123058 ], "stop": [ 123058 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00307E52CF", "query_cov": 1.0, "subject_cov": 0.7452830188679245, "identity": 0.975, "score": 146.0, "evalue": 4.47e-44, "gene_start": 1, "gene_end": 79, "reference_start": 1, "reference_end": 79, "reference_sequence": "MENKILLAEHENSTVVAEFKSIKVHSNKFESEAELEEAFIKLLIAQGYEYLPIKNNNDLKNNLRKQIEKLNNYQFTNNEWESFLSTYLLNPNDGIEARSKKNSWRL" }, "paralog": false, "identity": 0.9702970297029703, "score": 196.0, "evalue": 3.18e-66, "description": "Internal stop codon at 123058. Nonsense mutation around 123058.", "psc": { "uniref90_id": "UniRef90_UPI00307E52CF", "product": "type I restriction endonuclease", "uniref50_id": "UniRef50_UPI00307E52CF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00307E52CF", "UniRef:UniRef50_UPI00307E52CF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00307E52CF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307E52CF" ], "product": "type I restriction endonuclease" } }, "id": "GPKLONFDEH_242", "locus": "GCA000006625_00202" }, { "type": "cds", "sequence": "contig_1", "start": 123316, "stop": 123837, "strand": "-", "frame": 1, "gene": null, "product": "Type I restriction enzyme S protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "RefSeq:WP_010891674.1", "SO:0001217", "UniParc:UPI00000D4015", "UniRef:UniRef100_Q9PR48", "UniRef:UniRef50_Q9PR48", "UniRef:UniRef90_Q9PR48" ], "nt": "TTGTCATTTAAGCATATATCTGCAAACGAATTAAAACGATATGAAAAGTGTAATTTTGGTAATGTTAAACTTCTTTCAACAGGATTGTATGATGGATATATAAAATATAATGAAAATGATAATAATATTAATTATGGCGAAATAATTGCTTTGCCATCTGGTGGTTCTCCAATAATAAAATACTATAATGGTTATTTCATAGATAGTTTAAATATAATTTTTTCACAAAAAAACAAAAAAGAATGTAATTTAAAATTTATTTACTATTTTTTGATAGCAAATAAAATGCTTATTGAAGAAAATTATCGTGGTGCATCTGTAAAACATCCAAACATGATAGAGATTATTGAATTATTAATTCCTATCCCTCACATTTCAATCCAAAATAAAATTGTTGAAATCCTAGATAAATTAGAAGCATATACAAAAGATATTGGCGTAGGATTACCTTTTGAAATCAAACAACGCAAAAAACAATATGAATACTATCGAAATAAATTATTAGATTTTAAAAAATATTAA", "aa": "MSFKHISANELKRYEKCNFGNVKLLSTGLYDGYIKYNENDNNINYGEIIALPSGGSPIIKYYNGYFIDSLNIIFSQKNKKECNLKFIYYFLIANKMLIEENYRGASVKHPNMIEIIELLIPIPHISIQNKIVEILDKLEAYTKDIGVGLPFEIKQRKKQYEYYRNKLLDFKKY", "aa_hexdigest": "64034768b295b250ee5c8c9982a30661", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000D4015", "ncbi_nrp_id": "WP_010891674.1", "uniref100_id": "UniRef100_Q9PR48", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000D4015", "RefSeq:WP_010891674.1", "UniRef:UniRef100_Q9PR48" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR48", "uniref90_id": "UniRef90_Q9PR48" }, "psc": { "uniref90_id": "UniRef90_Q9PR48", "product": "Type I restriction enzyme S protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q9PR48", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_Q9PR48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR48" ], "product": "Type I restriction enzyme S protein" }, "genes": [], "id": "GPKLONFDEH_243", "locus": "GCA000006625_00203" }, { "type": "cds", "sequence": "contig_1", "start": 123975, "stop": 124238, "strand": "-", "frame": 2, "gene": null, "product": "Type I restriction enzyme S protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "RefSeq:WP_231140601.1", "SO:0001217", "UniParc:UPI000169D7B3", "UniRef:UniRef100_Q9PR47", "UniRef:UniRef50_A0AAC9T2H6", "UniRef:UniRef90_Q9PR47" ], "nt": "TTGAGAAAAATTACTAAAAAACATGTAAATCAAGCATCAGGAAATCCAAAATTAATGAGTAATGTTATGCAAGAAATCATAATCCCTATTCCGCCCATTTCAATCCAAAATAAAATTGTTGAGATTTTAGATAAATTGGAAACATATACGAAAGATATTAATACTGGACTTCCTCTTGAAATCGAACAACGTAAAAAACAATACGAATATTATCGTAATAAATTATTAGACTTTGATAATATAGCACGAGAGAGAGAGAGCTAA", "aa": "MRKITKKHVNQASGNPKLMSNVMQEIIIPIPPISIQNKIVEILDKLETYTKDINTGLPLEIEQRKKQYEYYRNKLLDFDNIARERES", "aa_hexdigest": "cfbcf4e418ad8ed2b6facc62edd5f6ca", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000169D7B3", "ncbi_nrp_id": "WP_231140601.1", "uniref100_id": "UniRef100_Q9PR47", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169D7B3", "RefSeq:WP_231140601.1", "UniRef:UniRef100_Q9PR47" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR47", "uniref90_id": "UniRef90_Q9PR47" }, "psc": { "uniref90_id": "UniRef90_Q9PR47", "product": "Type I restriction enzyme S protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AAC9T2H6", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2H6" ], "product": "Type I restriction modification DNA specificity domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_244", "locus": "GCA000006625_00204" }, { "type": "cds", "sequence": "contig_1", "start": 124317, "stop": 125363, "strand": "+", "frame": 3, "gene": "hsdM", "product": "type I restriction-modification system subunit M", "db_xrefs": [ "COG:COG0286", "COG:V", "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0009307", "KEGG:K03427", "RefSeq:WP_006689008.1", "SO:0001217", "UniParc:UPI00000D4017", "UniRef:UniRef100_Q9PR46", "UniRef:UniRef50_Q9PR46", "UniRef:UniRef90_Q9PR46" ], "nt": "GTGTTCGGTGATGCTTATGAATACTTAATGAGTATGTATGCTGCAAATGCAGGTAAATCAGGGGGAGAATTTTTCACCCCTCAAGAAGTTAGTGAACTATTAGTAGAATTAACATTAATTGATTTTAATAATGAAAATAAAGATGTTAGAAGAAAAATTGGAAAAGTTTATGATCCATGTTGTGGTTCTGGATCATTACTTTTAAAGTATGCAAAATTAAATGAAGGAGTTAAATTTTATGGTCAAGAAATTAATCTTACTACGTATAATTTAGCAAGAATTAATATGTTTTTGCACAATATAGGTTATGATAAATTTGATATCAAATTAGGTGATACATTACTAGATCCTAAACATAATGATGATAAACCTTTTGATGCAATTGTTTCAAATCCTCCATATTCAACAAAATGGGAGGGAAAATCAAATCCTTTATTAGCTAACGATGAGCGATTTCATGTTACTCAATTAGCACCTAAAGGAAAAGCTGATTTTGCTTTTGTATTGCATATACTTCATAATTTATCTTCTAGTGGAACAGCAGCTATAGTTATGTTTCCAGGAACTTTATATCGAGATCATGCAGAACAAGATATTAGAAAATATTTAGTTGATAATGTTAATGTTGTAGATAGTGTGATTCAATTACCAGATAATCTGTTTTTTGGAACAAGTATTTCAACATGTATTATAGTTTTAAGAAAAAACAAAAACAACAATGATAATGCTAATGGTATCTTGTTTGTTGATGCATCAAAAGAATTTGTTAAATCTGGTATCAAAAACAAATTAACCAATGCTAATATTAAAAAAATAGTTGATACGATTCGTTTCAAAAAAGAAGTAACTTATTTTTCAAAACTTGTATCACGAGAAGAAGTTAAAAATAAAAACTATAATTTATCAGTTAACACATATGTGGAAAAAGAAGACACGTCAGAAAAAATTGATATTAAATTATTAAATATGCAAATTAAAGAAATTGTGGCTAAAATCGAAAAGTTAAGAAAAGAAATTGATGAAATTGTTTTAGAATTAGAAGAATAA", "aa": "MFGDAYEYLMSMYAANAGKSGGEFFTPQEVSELLVELTLIDFNNENKDVRRKIGKVYDPCCGSGSLLLKYAKLNEGVKFYGQEINLTTYNLARINMFLHNIGYDKFDIKLGDTLLDPKHNDDKPFDAIVSNPPYSTKWEGKSNPLLANDERFHVTQLAPKGKADFAFVLHILHNLSSSGTAAIVMFPGTLYRDHAEQDIRKYLVDNVNVVDSVIQLPDNLFFGTSISTCIIVLRKNKNNNDNANGILFVDASKEFVKSGIKNKLTNANIKKIVDTIRFKKEVTYFSKLVSREEVKNKNYNLSVNTYVEKEDTSEKIDIKLLNMQIKEIVAKIEKLRKEIDEIVLELEE", "aa_hexdigest": "b0c8788a6e26e2726c056ff7a5012a36", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000D4017", "ncbi_nrp_id": "WP_006689008.1", "uniref100_id": "UniRef100_Q9PR46", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000D4017", "RefSeq:WP_006689008.1", "UniRef:UniRef100_Q9PR46" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR46", "uniref90_id": "UniRef90_Q9PR46" }, "psc": { "uniref90_id": "UniRef90_Q9PR46", "gene": "HsdM", "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_Q9PR46", "cog_id": "COG0286", "cog_category": "V", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR46" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [ "hsdM" ], "id": "GPKLONFDEH_245", "locus": "GCA000006625_00205" }, { "type": "cds", "sequence": "contig_1", "start": 125367, "stop": 126170, "strand": "+", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00017250EC", "UniRef:UniRef90_UPI00017250EC" ], "nt": "ATGAATAAAATTGAAAAATTAATTAATGAGTTCTGCCCTAATGGAGTGGAGTTTAAAAAATTAAAAAATATAATTACAGTGGCTCCTAAATCACCTTTTGGAGTTACAAAACTTTTAAAAATGGAAAAGGGAAATTATTTAACAATAACATCAGGGAAGAAATCATTTTATGTTGACAATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGCGGTCAAGCAGATATAAAATATAATTTTGGAAAAACAATGTATTCTGATCATATATTCGCATTCAAAGTAAATGAATATAACACAAAATATCTTTACTTCTATCTATTAAATATTTCTAATTTTATTAATAAAAAATTGTTTATAGGTAGTACATTAAAAAATTTAAATAAAAAAGAATTTTTAAATCTTGCAATCCCTATTCCGCCCATTTCAATCCAAAATAAAATTGTTGAAATATTAGACAAGTATACAGAATTAGAAACAGAATTAGAAACAGAATTAGAAACAGAATTAGAACTTAGAAATAAGCAGTATATATATTATCGCAATGAATTACTAAACTTTAATAAAAATCAAACATTATTAAAGAAAATAATGGACAGTGATGATATAAAATCTATTGATCATAAAATTGAGTTTAAAAAGATTGAATATGTGTGTGAAATAAAAAGAGGTCAAGTATACTCTAAGGAATTTATTAATTCTAATAAAGGAAATTATCCAGTATATTCATCACAAAGTTTAAATGATGGTGTTTTAGGAAATATTAATAAATATGATTTTGATGGTGAATATGTTACTTGA", "aa": "MNKIEKLINEFCPNGVEFKKLKNIITVAPKSPFGVTKLLKMEKGNYLTITSGKKSFYVDNFLVDGEYIFVNDGGQADIKYNFGKTMYSDHIFAFKVNEYNTKYLYFYLLNISNFINKKLFIGSTLKNLNKKEFLNLAIPIPPISIQNKIVEILDKYTELETELETELETELELRNKQYIYYRNELLNFNKNQTLLKKIMDSDDIKSIDHKIEFKKIEYVCEIKRGQVYSKEFINSNKGNYPVYSSQSLNDGVLGNINKYDFDGEYVT", "aa_hexdigest": "ab997238ff58fb07b585aaf3405211de", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI00017250EC", "query_cov": 1.0, "subject_cov": 0.898989898989899, "identity": 0.978, "score": 495.0, "evalue": 3.98e-176, "valid": true, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00017250EC", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_UPI00017250EC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00017250EC" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "GPKLONFDEH_246", "locus": "GCA000006625_00206" }, { "type": "cds", "sequence": "contig_1", "start": 126545, "stop": 126874, "strand": "+", "frame": 2, "gene": "hsdM", "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "COG:COG0286", "COG:V", "SO:0001217", "UniRef:UniRef50_Q9PR44", "UniRef:UniRef90_Q9PR44" ], "nt": "ATGAAAATAACCTTAATTAAAGAAAAAGGTTTTTATATTAAGCCGTCATTTTTGTTTGCTAATATTGTTAAAAATGCTGAAAAAAATGAAAATTTAAATGAAACTTTGGAAACTGTTTTTAACAATATTGAAGGATCATCCTCTGGTTATGATTCTGAAAAAAATTTAAAAGGGTTATTTAATGATATTGATGTTAATAATAGTCGTTTAGGAAGTACTACACAAGAAAGAAATAAAAGACTAGCAAGAATTTTAATTAGAATTATATATAAAAATATGGATTTATTAATTTTAAAACCATATATAAATAAAAAAGAGATTCCTAATTAA", "aa": "MKITLIKEKGFYIKPSFLFANIVKNAEKNENLNETLETVFNNIEGSSSGYDSEKNLKGLFNDIDVNNSRLGSTTQERNKRLARILIRIIYKNMDLLILKPYINKKEIPN", "aa_hexdigest": "c06521abe5d973c6eca21c4dbdd0f354", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdM" ], "truncated": "5-prime", "pseudogene": { "start": 126311, "stop": 126874, "observations": { "mutation": [ 126359, 126398 ], "stop": [ 126359, 126398 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR44", "query_cov": 1.0, "subject_cov": 0.5828877005347594, "identity": 1.0, "score": 211.0, "evalue": 5.18e-68, "gene_start": 1, "gene_end": 109, "reference_start": 79, "reference_end": 187, "reference_sequence": "MDNKKEIERNNLHATIWKIADELRGAIDGWDFKQYVLGILFYRYISENLTKYINDNEHKIGDHDFNYETCNDDDVDEEMKITLIKEKGFYIKPSFLFANIVKNAEKNENLNETLETVFNNIEGSSSGYDSEKNLKGLFNDIDVNNSRLGSTTQERNKRLARILIRIIYKNMDLLILKPYINKKEIPN" }, "paralog": false, "identity": 0.9893048128342246, "score": 371.0, "evalue": 1.1e-133, "description": "Internal stop codon at 126359, 126398. Nonsense mutation around 126359, 126398.", "psc": { "uniref90_id": "UniRef90_Q9PR44", "gene": "HsdM", "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_Q9PR44", "cog_id": "COG0286", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR44", "UniRef:UniRef50_Q9PR44", "COG:COG0286", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR44" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" } }, "id": "GPKLONFDEH_247", "locus": "GCA000006625_00207" }, { "type": "cds", "sequence": "contig_1", "start": 126912, "stop": 127478, "strand": "-", "frame": 2, "gene": null, "product": "putative protein UU101", "db_xrefs": [ "RefSeq:WP_006689154.1", "SO:0001217", "UniParc:UPI0000139277", "UniRef:UniRef100_Q9PR43", "UniRef:UniRef50_Q9PR43", "UniRef:UniRef90_Q9PR43" ], "nt": "ATGGCTAAATACATTAAAACAGGTGTTTCTTACATTAATTTAGACAACGCACGTACAATTAATATTCTTCCAGAAGATATTGATTCATATTTAGAACTTGGTGGAGATGAAGCTTATAAAACAAGCGATCTAGGATCAGAGTTGTATATTAATTATGCTGATTTTGAATCAACAAACATTTTATTTGATCTTAAAAAAGAAGAGTTACAAGCAAAAATTGATGCTTTTTTAATTTCTAATGATACAGTATTAGATTTATCAGAAGTTTTCTTAGATATTCATTTTAGTGAAGATGATGATTATGAAGAAGATTGCTGTTGTGAAGATGAATGCTGTAGCGATGAAGAAAATGAAGCTTGTTGTAATTCAGAAGTAAAAGTTGAAGAAGAATGCTGTGGCGGAGCTAAAGATGATTGTTGTGGTGGTCACGAACATGAACATGAAGTTTGCTGTGACTCGGAAACAAAAACATCAGAAACCCAAGAAGAATGTTGTGGTGGAACTAAAGATGATTGCTGTGGTGGTCACGAACATGAACACCATCATCATCACAGTCACCAACATTAA", "aa": "MAKYIKTGVSYINLDNARTINILPEDIDSYLELGGDEAYKTSDLGSELYINYADFESTNILFDLKKEELQAKIDAFLISNDTVLDLSEVFLDIHFSEDDDYEEDCCCEDECCSDEENEACCNSEVKVEEECCGGAKDDCCGGHEHEHEVCCDSETKTSETQEECCGGTKDDCCGGHEHEHHHHHSHQH", "aa_hexdigest": "641193f7831e345340b6b0f6542e0a17", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000139277", "ncbi_nrp_id": "WP_006689154.1", "uniref100_id": "UniRef100_Q9PR43", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139277", "RefSeq:WP_006689154.1", "UniRef:UniRef100_Q9PR43" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR43", "uniref90_id": "UniRef90_Q9PR43" }, "psc": { "uniref90_id": "UniRef90_Q9PR43", "product": "Uncharacterized protein UU101", "uniref50_id": "UniRef50_Q9PR43" }, "pscc": { "uniref50_id": "UniRef50_Q9PR43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR43" ], "product": "Uncharacterized protein UU101" }, "genes": [], "id": "GPKLONFDEH_248", "locus": "GCA000006625_00208" }, { "type": "cds", "sequence": "contig_1", "start": 127704, "stop": 128663, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17760.6" ], "nt": "ATGGACAAAATTATCATTAAAGGCGCTAGAGAAAATAATTTAAAAAATATTGATCTAGAAATTCCTAAAAATAAACTAGTTGTTATTACAGGTGTTTCAGGATCAGGTAAATCATCGCTTGCTTTTGATACGATTTTTGCTGAAGGAAAACGTCGTTATTTTGAATCACTTTCAAGTTATGCCCGCCAGTTTTTAGGTGGAAATGATAAAGCTGATGTTGAAAGTATTGAAGGTTTATCACCAACCATTGCTGTTGATCAAAAATCAACAAATCAAAATCCAAGATCAATTGTTGGGACAATTACAGAAATCTATGATTATTTACGAGTTTTATTTGCTCGTGTTGGAACACCTTTTTGTCCAAATGGGCATGGTCAAATTAAATCACAAACTCCAAAACAAATTGCTGATTTCTTATTTAGCTTATCACCTAAAAGTAAAATACAAATTCTTGCACCAATTGAAATTAAAAAAGGTTATAAAATTAATGATGTTTTAAATAATTTAAGAAATCAAGGTTATTTAAGAGTTTTAGTTAATAATGAAGTTTATCAATTAGATGAAAATTTGCCTCAATTTTTAGATAACAAAAAAACAGATGTTGCAATCATTGTTGATCGTTTAATTTTAAATATTGATCACCAAACAAAAACACGAGCACTAGATGCTATTGAATTTGCTTTAAATTATAGTGGGGGAGAAATTGCATTTAAGGTTAATGATGATATTCATTACTTTACACAAAACGATGTTTGTCAAGTTTGTGGTTTTAAAATTAAAGAAATTGAACCAACATTATTTTCATTTAATTCACCAATTGGTGCTTGTGAACAGTGTAAAGGGCTAGGATATAATTATGTTCCTGATGAGCGTAAAATGATCCCTAATCCAAATTTATCAATTAACGAAGGCGGTTTAGATTATTTTAAAAACACTGTTAATACAACTAATCTTGATTGA", "aa": "MDKIIIKGARENNLKNIDLEIPKNKLVVITGVSGSGKSSLAFDTIFAEGKRRYFESLSSYARQFLGGNDKADVESIEGLSPTIAVDQKSTNQNPRSIVGTITEIYDYLRVLFARVGTPFCPNGHGQIKSQTPKQIADFLFSLSPKSKIQILAPIEIKKGYKINDVLNNLRNQGYLRVLVNNEVYQLDENLPQFLDNKKTDVAIIVDRLILNIDHQTKTRALDAIEFALNYSGGEIAFKVNDDIHYFTQNDVCQVCGFKIKEIEPTLFSFNSPIGACEQCKGLGYNYVPDERKMIPNPNLSINEGGLDYFKNTVNTTNLD", "aa_hexdigest": "f7ba15b8bd433617c73ec53a1901f4d3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00261747A6", "query_cov": 0.9529780564263323, "subject_cov": 0.95, "identity": 0.513, "score": 300.0, "evalue": 7.58e-98, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A9D2IF50", "kegg_orthology_id": "K03701" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17760.6", "name": "UvrA_inter", "length": 112, "aa_cov": 0.3510971786833856, "hmm_cov": 0.9732142857142857, "evalue": 1.519954892920057e-28, "score": 94.45779418945312, "start": 130, "stop": 241 } ], "seq_stats": { "molecular_weight": 35712.21819999999, "isoelectric_point": 6.360192298889159 }, "id": "GPKLONFDEH_249", "locus": "GCA000006625_00209" }, { "type": "cds", "sequence": "contig_1", "start": 128907, "stop": 130535, "strand": "+", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "nt": "ATGAGTGAAAAAGTTTGTAAATCATGTAAAGGCAAAAAACTATCTTCACAAGCTTTATCGGTTTTAATTAATAAAATTAACATTATTGATTTTATTGAAAAAAACATTGATGAGGCGATTGATTTTTTATTACACTTAGATTTGAATGAAGCACAAACAAAAATTGCCAATCCAATTTTAAAAGAAGTTTTAGATCGTTTAGGATTTTTAAAAAATGTTGGTTTAGAATATTTAACTTTAGCTCGTCCTGCATCAAGTTTATCAGGGGGCGAAGCACAACGGATTCGTTTAGCCACTCAAATCGGCTCTAAACTAACAGGTATTTTATATGTACTTGATGAACCATCAATTGGTTTGCATCAACGCGATAACGATAAATTAATTAATACATTAAAAGAAATGCGTGATTTAGGTAATACAGTTATAGTTGTTGAACATGATGAAGAAACAATGCTAGCAGCTGATTATTTAATAGATATTGGTCCTCAAGCTGGAGTTAATGGTGGTTATGTAATAGCAGCTGGAACACCACAAGAAGTAATGCAAAATCCTAATTCACTAACAGGACAATATTTATCAAAACAAAAAGATATTTTAGTTCCTAAAACACGAAGAAGTGGTAATGGTCATAAAGTTATTTTAAAAGGTGCAAAACATAATAATTTAAAAAATGTTGATTTAACAATTCCTTTAGGTAAATTCATTTGTGTTACTGGAGTTTCAGGATCTGGTAAATCATCACTAATTTTAGAAACATTAGTTAAAGCTATTGAATACACTAATTTTAATCCTTTTGTAATTCCAGGCGAGTATAAAGATTTAATTGGCGCATCGAATGTTGATAAAATCGTTGTCGTTAATCAAGATGCAATTGGTAGAACTACGAGATCAAATCCAGCTACTTATGTTGGGGTATTTGATGATATTAGAACAGTATTTGAAAATACAATTGAAGCTAAAGCACGTGGCTATAGTAAAAGTCGTTTTTCTTTTAATATTAAAGGAGGCCGTTGTGAACGTTGTTGGGGGGATGGTACGATTCGCATTGAGATGCACTTTTTACCAGATGTTTATATAAGTTGTGAAGAATGTCATGGAAAACGTTATAACGATGAAACACTTCAAGTTAAATTTAAAGGTAAATCAATTTATGATGTACTAAAAATGCCAATCGATGAAGCTTTAGTATTTTTTGAAAATTATCCTGGTATTCATCGTAAACTACAATTATTAGTTGATGTTGGTTTAGGATATTTAGAGTTAGGAGCCTCTTCAACTTCATTATCAGGGGGCGAAGCACAACGTATTAAGTTAGCTAAGTTTTTACAACGTAAGCCAACAGGAAAAACATTATTTGTTTTAGATGAACCAACAACAGGACTGCATATTGATGATGTAGCTAAATTAATTAAAATTTTAGATAAAATTGTTGATGGAGGAGACACTGTTTTAGTTATAGAACACAATCTTGATTTAATTAAGGTTGCAGATTATATTATTGATATTGGCCCTGAAGGTGGTAATAAAGGTGGTAAAATTATAGCTACTGGTACTCCAGAGCAATTACTAAGTAAAAAGGACATATCTTATACTGCTCAATATTTAGAAAAGTATTTAAAAAAGAACTAA", "aa": "MSEKVCKSCKGKKLSSQALSVLINKINIIDFIEKNIDEAIDFLLHLDLNEAQTKIANPILKEVLDRLGFLKNVGLEYLTLARPASSLSGGEAQRIRLATQIGSKLTGILYVLDEPSIGLHQRDNDKLINTLKEMRDLGNTVIVVEHDEETMLAADYLIDIGPQAGVNGGYVIAAGTPQEVMQNPNSLTGQYLSKQKDILVPKTRRSGNGHKVILKGAKHNNLKNVDLTIPLGKFICVTGVSGSGKSSLILETLVKAIEYTNFNPFVIPGEYKDLIGASNVDKIVVVNQDAIGRTTRSNPATYVGVFDDIRTVFENTIEAKARGYSKSRFSFNIKGGRCERCWGDGTIRIEMHFLPDVYISCEECHGKRYNDETLQVKFKGKSIYDVLKMPIDEALVFFENYPGIHRKLQLLVDVGLGYLELGASSTSLSGGEAQRIKLAKFLQRKPTGKTLFVLDEPTTGLHIDDVAKLIKILDKIVDGGDTVLVIEHNLDLIKVADYIIDIGPEGGNKGGKIIATGTPEQLLSKKDISYTAQYLEKYLKKN", "aa_hexdigest": "ec6bc1385cd1cfef4a00ebea0d33877a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGE6", "query_cov": 0.9317343173431735, "subject_cov": 0.9960552268244576, "identity": 0.6629999999999999, "score": 683.0, "evalue": 2.95e-242, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0PGE6", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PGE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "product": "UvrABC system protein A" }, "genes": [], "id": "GPKLONFDEH_250", "locus": "GCA000006625_00210" }, { "type": "cds", "sequence": "contig_1", "start": 130540, "stop": 130872, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACATAAGACAAAAAAAATAACGCTTTTAATAGCAGGAATTACTTTAACTTCATTATGTACAATTTTTGCTATTACAGCTTGTAGTAAGCAAAAATCACAACCAAATCCAAAAATAAAACCCAATAAAATTATTGAGAAAATTGATCAAGCATTTTTTAATGAATTTAAAACAAAAATTGAACAAAAACGCACACTTCCAAATATTAAAATCACTTCAACCTTTAAAATTCCTTTTGAGAAAAACGAAAACACTTTAAAATCCCCACTTTTTAAAATTGGTGAGAAATCGGTTTTTGATTTAAAACAAAATGAAATTCCAATAACATGA", "aa": "MKHKTKKITLLIAGITLTSLCTIFAITACSKQKSQPNPKIKPNKIIEKIDQAFFNEFKTKIEQKRTLPNIKITSTFKIPFEKNENTLKSPLFKIGEKSVFDLKQNEIPIT", "aa_hexdigest": "faff9454f65313395f0710d596cb0c53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12591.84009999998, "isoelectric_point": 9.930597114562985 }, "id": "GPKLONFDEH_251", "locus": "GCA000006625_00211" }, { "type": "cds", "sequence": "contig_1", "start": 131083, "stop": 131223, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATGGTATTTTACGTTCAGTATATCGTATCGCTAATCCTAAACTTGCAATCAAGGATTTTTACCTTGATGGTTCGCTTTATGGATTTAAAAAAGAAAAAAGTAAATACATGAATTTTACAAGTTTGTTTCAATGA", "aa": "MQNGILRSVYRIANPKLAIKDFYLDGSLYGFKKEKSKYMNFTSLFQ", "aa_hexdigest": "5e1907f12d5596b45973ed11c12d3e72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5425.2853000000005, "isoelectric_point": 9.82177448272705 }, "id": "GPKLONFDEH_252", "locus": "GCA000006625_00212" }, { "type": "cds", "sequence": "contig_1", "start": 131410, "stop": 132204, "strand": "+", "frame": 1, "gene": null, "product": "Iron ABC transporter substrate-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D789", "UniRef:UniRef90_A0AAC9T332" ], "nt": "ATGAATGCCAAGAATTTGATTAATCCCAACAACCCTAATATTGCAAAAATTTTTAAAATTAAAGTTTTTAAAGAAAACGAAAAATTTAAACCAATAACCTTAACGTCAATTAATCAACAAGAAGCGGATGAATATGATCGACAAAGAAATCAAATTATTCATAAAAATAATTCAGAAATTGGGATATTAGATGATTCAAATAAAATTAAAGAGTTAGGAACTTTTTTATATTTTAAAAAACCTTTATCTGAAATTACAATCAACGATTTTGATCGTTATTTTAGAACTTATTTTATTTGTCCAGAGCAAGATTTTAATGATTTTAGTCGTCAATATAAAATTACTAAAATTGATAAACAAAAACAAATAATAGAACTAAGTATATTACATATTAATAATAAAACAAATGAAATTTATAGTTCACCAATTAATTTTAAGTTTTATTACAAAAAAATATATGATATTAACAAAAACACTGATGCACGATTAGATTTAAGTTTTGATGAATCTAAAAAAGAACAAAAACAAAAACTACAAGAGTTAGTTAATAAATTAAATTTTGATAAACTTACTTATAATGATTTAAGTGAATTAGCTGTTGTGATGTTTGAGGATGTGGATTTAGAAAACACTAAAATTTATGTTGAAATGATTAAAAATAAAGATCGTTCAATTACTTTAAAAGCTTTTGTTGATAAAGCTTTTAAAAATGGAAAATTAAAGGTAAGTAATAAACCAGATATTTTAATAAAAGAGTTTAAAATAAACTATTTTATGCCTAATTACTTTAAATAA", "aa": "MNAKNLINPNNPNIAKIFKIKVFKENEKFKPITLTSINQQEADEYDRQRNQIIHKNNSEIGILDDSNKIKELGTFLYFKKPLSEITINDFDRYFRTYFICPEQDFNDFSRQYKITKIDKQKQIIELSILHINNKTNEIYSSPINFKFYYKKIYDINKNTDARLDLSFDESKKEQKQKLQELVNKLNFDKLTYNDLSELAVVMFEDVDLENTKIYVEMIKNKDRSITLKAFVDKAFKNGKLKVSNKPDILIKEFKINYFMPNYFK", "aa_hexdigest": "8b81aa161daed45b79fd61fb5ef4c757", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 131110, "stop": 132204, "observations": { "mutation": [ 131221 ], "stop": [ 131221 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T332", "query_cov": 1.0, "subject_cov": 0.47653429602888087, "identity": 0.992, "score": 508.0, "evalue": 1.95e-177, "gene_start": 1, "gene_end": 264, "reference_start": 291, "reference_end": 554, "reference_sequence": "MKHKTKKITLLIAGITLTSLCTIFAITACSKQKSQPNPKIKPNNVVEKVDQAFFNEFKTKIEQKRALPNIKITSTFKIPFERNENTLKSPLFKIGEKSVFDLKQNEIPITWVQDLKDYFLSQKTILTYYSLANLKLTGNDAKDAYFLEFIQYIQNMQQKGIILEDFYQQLWYLISEISDYDMQNGILRSVYRIANPKLAIKDFYLDGSLYGFKKEKSKYMNFTSLFQWPNAEEKATFDVVFKNKNSAQSYTKKIELIYSKEKKFILDSLINYSEFTPGEYELVSIKKHDDMNAKNLINPNNPNIAKIFKIKVFKENEKFKPITLTSINQQEADEYDRQRNQIIHKNNSEIGILDDSNKIKELGTFLYFKKPLSEITINDFDRYFRTYFICPEQDFNDFSRQYKITKIDKQKQIIELSILHINNKTNEIYSSPINFKFYYKKIYDINKNTDARLDLSFDESKKDQKQKLQELVNKLNFDKLTYNDLSELAVVMFEDVDLENTKIYVEMIKNKDRSITLKAFVDKAFKNGKLKISNKPDILIKEFKINYFMPNYFK" }, "paralog": false, "identity": 0.9917582417582418, "score": 724.0, "evalue": 7.83e-265, "description": "Internal stop codon at 131221. Nonsense mutation around 131221.", "psc": { "uniref90_id": "UniRef90_A0AAC9T332", "product": "Iron ABC transporter substrate-binding protein", "uniref50_id": "UniRef50_A0AAP9D789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T332", "UniRef:UniRef50_A0AAP9D789" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D789" ], "product": "Iron ABC transporter substrate-binding protein" } }, "id": "GPKLONFDEH_253", "locus": "GCA000006625_00213" }, { "type": "cds", "sequence": "contig_1", "start": 132231, "stop": 132353, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAAATCAAAAAACAAGAAATGATGGTATAAAAATGAAAGATATTGATCCAAGAATAAAAGAGGTTTTGATCACTGAAGAACAAATTGATCAAAAAATCACTGAAGCAGCTAATTGA", "aa": "MIKNQKTRNDGIKMKDIDPRIKEVLITEEQIDQKITEAAN", "aa_hexdigest": "0b8f0c7b2728e348f4646757b14d4812", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4669.339300000001, "isoelectric_point": 6.125674629211424 }, "id": "GPKLONFDEH_254", "locus": "GCA000006625_00214" }, { "type": "cds", "sequence": "contig_1", "start": 132453, "stop": 132851, "strand": "+", "frame": 3, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "EC:2.4.2.8", "GO:0006166", "KEGG:K00760", "SO:0001217", "UniRef:UniRef50_Q9PR40", "UniRef:UniRef90_Q9PR40" ], "nt": "ATGAAACTAGATTTTTTAGCAATTAGTAGTTTTAAGGGAGCAACTAGTGCTCAAACAGAACCAGAAATTATTACTGATCTTAAATTTGAAGTTAAAGATCGTGATCTAATTTTAGTAGAAGATATTGTTGATACAGGACGCACAATTAAAAAGGTTTATGATTTACTTAAAATTCGAGGCGCTCGTTCAATTAAATTAGTTACCTTGGTTGATAAAAAAGACGGCCGATTAGTAGATTTACAAGCCGATTTTGTATGTTGTGATATTCCATTAGTCTTTATTGTTGGTTTTGGTTTAGATTATAAAGAAATTATGCGTAATTTACCATATATTGGTGTTTTAAAAGAAGAAGTTTATCAAAATGATTTAAATAATAAAAACGAAGGGGATGGAGAGTAA", "aa": "MKLDFLAISSFKGATSAQTEPEIITDLKFEVKDRDLILVEDIVDTGRTIKKVYDLLKIRGARSIKLVTLVDKKDGRLVDLQADFVCCDIPLVFIVGFGLDYKEIMRNLPYIGVLKEEVYQNDLNNKNEGDGE", "aa_hexdigest": "f7ba9926841866765777b60726c5ce08", "start_type": "ATG", "rbs_motif": null, "genes": [ "hpt" ], "truncated": "5-prime", "pseudogene": { "start": 132231, "stop": 132851, "observations": { "mutation": [ 132351 ], "stop": [ 132351 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR40", "query_cov": 1.0, "subject_cov": 0.6407766990291263, "identity": 1.0, "score": 261.0, "evalue": 4.02e-87, "gene_start": 1, "gene_end": 132, "reference_start": 75, "reference_end": 206, "reference_sequence": "MIKNQKTRNDGIKMKDIDPRIKEVLITEEQIDQKITEAANWINKEYEGKEPIIIGILKGCIPFIGKLLPKIKIDMKLDFLAISSFKGATSAQTEPEIITDLKFEVKDRDLILVEDIVDTGRTIKKVYDLLKIRGARSIKLVTLVDKKDGRLVDLQADFVCCDIPLVFIVGFGLDYKEIMRNLPYIGVLKEEVYQNDLNNKNEGDGE" }, "paralog": false, "identity": 0.9951456310679612, "score": 403.0, "evalue": 1.21e-145, "description": "Internal stop codon at 132351. Nonsense mutation around 132351.", "psc": { "uniref90_id": "UniRef90_Q9PR40", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_Q9PR40", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR40", "EC:2.4.2.8", "UniRef:UniRef50_Q9PR40", "KEGG:K00760", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR40" ], "product": "Hypoxanthine phosphoribosyltransferase" } }, "id": "GPKLONFDEH_255", "locus": "GCA000006625_00215" }, { "type": "cds", "sequence": "contig_1", "start": 132856, "stop": 132999, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTTTTTAAAAAAAATAGTAAATTTATTTAGTTCAAAAATTGAATCAGAAGATAATAATGTTAAAAAAGACGACTTAACTCAACCACGTAAACAATCGCCAGAAGCGCGCAAACGAAGAAATCGAAGAATTATATTTTGA", "aa": "MYFLKKIVNLFSSKIESEDNNVKKDDLTQPRKQSPEARKRRNRRIIF", "aa_hexdigest": "55ecc66f877f2f0f85abb6f9112cd2e3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5738.5858, "isoelectric_point": 10.432547950744627 }, "id": "GPKLONFDEH_256", "locus": "GCA000006625_00216" }, { "type": "cds", "sequence": "contig_1", "start": 133681, "stop": 135021, "strand": "+", "frame": 1, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "nt": "ATGTTATATGGACCTCCAGGAACAGGAAAAACATTAATTGCTAAAGCTGTAGCTGGTGAAGCTAATGTTCCATTCTTTCAAACAACGGGTTCATCTTTTGAAGATACTTTTGTTGGAGTTGGTGCACGTCGTGTGAGAGAATTATTTGAAAAAGCACGTAAGAGTGCTCCCGCCATTATTTTTATTGATGAAATTGACTCAGTAGCTAAAAAACGTGGTAATTCATTAACTGCTGTACAAGACCAAACAATTAACCAATTATTATCAGAATTAGATGGTTTTGATACTTCAAGTGGTGTAATTGTTATGGCTGCGACAAATAGATTAGACACATTAGATGACGCAATTTTACGTCCTGGACGTTTTGACCGACAAATTAGTGTTAACTTACCAGATATTTTAGAACGTGAACAAATTTTAAGAATTCACTCACGTAATAAAAACTTATCTGCGAAAGTTAGTTTAGAAGATATTGCACGTCGTACTGCTGGATTTAGTGGTGCACAATTAGAAAATGTTTTAAATGAAGCCGCATTACTATCAGTTAGAGATAAAGCAACATCAATTCATATGAATCATTTAGATGAAGCGATTGATCGTGTTATTGCAGGACCTTCACGTCCTAATAAAGTTATTTCTGAACGTGAACGTGAACAAGTTTCATACCATGAAGCTGGACATGCATTAATTGGTTTATATTCACCAGGTGCTGATGTTGTTCAGAAAATTACTATTGTTGCTCGTGGTCGAGCTGCTGGTTATACACTACAAACTCCTGAAAGAAATGAAAATATTTTACAAAATAAAACTGAATTAATTAGTCGAGTGCGTACTGCATTAGGTGGTCGAGCTGCAGAAGAGTTAATTTATGGACCTAATGAAATTACAACAGGTGCAGCAAATGACTTTTATAAAATTACAAACATCGTAAGAGCAATGGTAGCTTCTTTTGGAATGACAGATGTGGGTTTAACGCAATATATTGCTACTGAAGGTGTTGATAACCCTTATCGAAATAGTTATTCAGAACAAACAGCATTAGCAATTGATATTGAAATTGAAAAAATTATTCAACGTGAATATAAAATTGTTAAAGAAATGATTAATGAACATCGTGAAGAATTGGAATTAATCGTTCAAACATTATTAGAATTAGAAACAATTTTAAAGCCACAAATTGATTATATTCACCAATACAAACAATTACCACCAGAGGTAATTGCTAATAAAAACAAACGTGAAGCTTCTCAAAAACAAGCAAATAGTAGTGTAGAAGAAGCAAAAGTTGTTGACGATGAAGAATCAATAAAAGATAAAGAAAAAGATCAAAAATCTAACTAA", "aa": "MLYGPPGTGKTLIAKAVAGEANVPFFQTTGSSFEDTFVGVGARRVRELFEKARKSAPAIIFIDEIDSVAKKRGNSLTAVQDQTINQLLSELDGFDTSSGVIVMAATNRLDTLDDAILRPGRFDRQISVNLPDILEREQILRIHSRNKNLSAKVSLEDIARRTAGFSGAQLENVLNEAALLSVRDKATSIHMNHLDEAIDRVIAGPSRPNKVISEREREQVSYHEAGHALIGLYSPGADVVQKITIVARGRAAGYTLQTPERNENILQNKTELISRVRTALGGRAAEELIYGPNEITTGAANDFYKITNIVRAMVASFGMTDVGLTQYIATEGVDNPYRNSYSEQTALAIDIEIEKIIQREYKIVKEMINEHREELELIVQTLLELETILKPQIDYIHQYKQLPPEVIANKNKREASQKQANSSVEEAKVVDDEESIKDKEKDQKSN", "aa_hexdigest": "a338375b60ffd4881e5d2916cbf1760c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AS56", "query_cov": 0.9461883408071748, "subject_cov": 0.8559837728194726, "identity": 0.507, "score": 398.0, "evalue": 7.7e-132, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A449AS56", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AS56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "GPKLONFDEH_257", "locus": "GCA000006625_00217" }, { "type": "cds", "sequence": "contig_1", "start": 135132, "stop": 135557, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02403.27" ], "nt": "ATGTTTGATATTAATTTAATACGAAAAGATATTGCAACTACTAAAGAAAAGATGTTAAATAAAAAGGTGCCAAGCAATTTGTTTGATCAAATTTTTGATTTAGATGTTTTAGTACGAAGTTTAATGCAACAAGAACAAAATCTTAATGCTAAGAAAAATCAATTATCAAAAGAAATTGGTATTTTAGCTAAAAACAAAGATCCTAAATTACAACAAACATTAGATTTAGTTAATAATATTAAAAACGAATTGCAAGATATTTCATTAACATTATCAAACAAACAAGATGAATTAAATAAATTATTATTAGTAATTCCTAATATGCCAGATGATTCAGTGCCCATTGGCAATGATGAAAATGACAACGTCGAAATTAAAAAAGTTTTTAAACCAAAAAAATTTGATTTTTTACCCCTTGCGCATTGA", "aa": "MFDINLIRKDIATTKEKMLNKKVPSNLFDQIFDLDVLVRSLMQQEQNLNAKKNQLSKEIGILAKNKDPKLQQTLDLVNNIKNELQDISLTLSNKQDELNKLLLVIPNMPDDSVPIGNDENDNVEIKKVFKPKKFDFLPLAH", "aa_hexdigest": "e44c934cab2ee6724594f692e4d2cc58", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02403.27", "name": "Seryl_tRNA_N", "length": 109, "aa_cov": 0.7730496453900709, "hmm_cov": 0.9908256880733946, "evalue": 1.4467243604174653e-25, "score": 85.21244812011719, "start": 1, "stop": 109 } ], "seq_stats": { "molecular_weight": 16223.643699999986, "isoelectric_point": 6.591072273254395 }, "id": "GPKLONFDEH_258", "locus": "GCA000006625_00218" }, { "type": "cds", "sequence": "contig_1", "start": 135831, "stop": 136151, "strand": "+", "frame": 3, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003418C7B1" ], "nt": "GTGCAATTAACAAATTTACATCGTAATGAAATTTTAAAAGCTAGTGATTTACCTTTATACTATACAGCTCTAACACCTTGTTTTCGTAGTGAAGCAGGATCTGCAGGACGCGATGTTAGAGGTGTAATTCGTCAACACCAATTCCATAAAGTAGAATTAGTAAAATTATGTAAACCAGAAGATAGTTTTAAAGAATTAGAATCAATGACAAGACAGGCTGAAAGTATTTTAGAAGCTTTAGAATTACCTTATAGAAGAATCGTTTTATGCACAGGCGATTTAGGTTTTAGTTCAGCTAAAACATATGATTTAGAAGTTTGA", "aa": "MQLTNLHRNEILKASDLPLYYTALTPCFRSEAGSAGRDVRGVIRQHQFHKVELVKLCKPEDSFKELESMTRQAESILEALELPYRRIVLCTGDLGFSSAKTYDLEV", "aa_hexdigest": "4770f0a50c1e985171ad26901e7db99e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003418C7B1", "query_cov": 0.9811320754716981, "subject_cov": 0.8125, "identity": 0.654, "score": 132.0, "evalue": 1.13e-37, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_UPI003418C7B1", "kegg_orthology_id": "K01875" }, "pscc": { "uniref50_id": "UniRef50_UPI003418C7B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003418C7B1" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "GPKLONFDEH_259", "locus": "GCA000006625_00219" }, { "type": "cds", "sequence": "contig_1", "start": 136791, "stop": 137396, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGAAAAAACACTCCTACATCTTAGAGATATTACAAAAATCTATGATGATGGATTTGCTGCTGTAAACAAATTTGATTTAAAAATAAAAAAAGGTGAATTTGTCACTTTATTAGGACCAAGTGGTTGTGGTAAGACAACAATGTTAAAAATTATTGCTGGATTTGAACAACCTACTAATGGAAAAATTTTATATAATGGAATTGATATTAAGGATATGCCAATTCGACTAAGACCAACATCAACGGTGTTTCAAGACTATGCGCTATTCCCTAATATGACTGTTAAACAAAACATAAAATATGGTTTAAAACTAATGCGTAAACCAAAAGATAATGTTGATCAAAGTATTTATTTGCAAGCTGATAAAGTTTATAATTCAGCTTCTAAAAAAGCAAATGAAAAAATTAAAGAATTAAAAAAACAAAGACGCGGGTTATTGGCTGAAATCAAAAAAATGGATCTTAAATACCAAAAGAATAAGAACATTTTTGAAATAAAAGAAATGCGAAAAAACCAATATCTTGGCACACTTGATGAACTTTATCAAAAACAAGGTATTAATAAAAATGGAAAACCAGGTTTTTTAAATTATTTTAAAAGTTGA", "aa": "MEKTLLHLRDITKIYDDGFAAVNKFDLKIKKGEFVTLLGPSGCGKTTMLKIIAGFEQPTNGKILYNGIDIKDMPIRLRPTSTVFQDYALFPNMTVKQNIKYGLKLMRKPKDNVDQSIYLQADKVYNSASKKANEKIKELKKQRRGLLAEIKKMDLKYQKNKNIFEIKEMRKNQYLGTLDELYQKQGINKNGKPGFLNYFKS", "aa_hexdigest": "94af7db8b18ae48d5785397851ec9604", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 118, "aa_cov": 0.5771144278606966, "hmm_cov": 0.6694915254237288, "evalue": 4.5492863682573437e-23, "score": 77.9587631225586, "start": 23, "stop": 138 } ], "seq_stats": { "molecular_weight": 23240.02250000001, "isoelectric_point": 9.819195747375485 }, "id": "GPKLONFDEH_260", "locus": "GCA000006625_00220" }, { "type": "cds", "sequence": "contig_1", "start": 137412, "stop": 137534, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTAAACGATCCAATTGATAGAGAAATTTTTAATTTAAAAAAAGCATATAAAGAAAAAAGTGGATTAGATAAACGATATGATAAGATTACTTACAAATATAATGATCTTGATTATTGA", "aa": "MNLNDPIDREIFNLKKAYKEKSGLDKRYDKITYKYNDLDY", "aa_hexdigest": "075096705fb3af9cf191799c4873c087", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4932.520200000002, "isoelectric_point": 7.941489601135254 }, "id": "GPKLONFDEH_261", "locus": "GCA000006625_00221" }, { "type": "cds", "sequence": "contig_1", "start": 137703, "stop": 137978, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D25CD8" ], "nt": "ATGCAACAACGTGTTGCACTAGCACGTTCACTAGTAATACAGCCTGAAATTATTTTATTAGATGAACCATTAAGTGCACTAGATGCAAAAGTACGTAAACAATTACAAGATGAATTAAAAAAACTACATAAAAATTTAGGAATCACCTTTATTTTAGTAACTCATGATCAAGAAGAGGCTTTAAGTTTATCAGATAAAGTTGTGGTTATGTCAAATGGTCAAATCGAACAAGTCGGTAAACCAAGTGATATTTATGATTCACCAAATTCATTGTGA", "aa": "MQQRVALARSLVIQPEIILLDEPLSALDAKVRKQLQDELKKLHKNLGITFILVTHDQEEALSLSDKVVVMSNGQIEQVGKPSDIYDSPNSL", "aa_hexdigest": "2cc3bab9b23f163ffa50926492c45273", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D25CD8", "query_cov": 0.9230769230769231, "subject_cov": 0.9767441860465116, "identity": 0.631, "score": 111.0, "evalue": 2.11e-30, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI0034D25CD8" }, "pscc": { "uniref50_id": "UniRef50_UPI0034D25CD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D25CD8" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_262", "locus": "GCA000006625_00222" }, { "type": "cds", "sequence": "contig_1", "start": 138072, "stop": 138203, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAATGGTTTTAGCGAAAATGAAGCGTGTTATATTATGATTCGCCCAGAAGACTTTGATGTTGTTAAAAAAGACGAAGGATCAATTAATGCTAGAGTTGAATCAGTATTATATAAAGGGTTAATGTGA", "aa": "MINGFSENEACYIMIRPEDFDVVKKDEGSINARVESVLYKGLM", "aa_hexdigest": "9d9ed94bf0b52c142b4b809875e54a3d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4912.5949, "isoelectric_point": 4.554986000061036 }, "id": "GPKLONFDEH_263", "locus": "GCA000006625_00223" }, { "type": "cds", "sequence": "contig_1", "start": 138334, "stop": 138675, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAGCAATTTAATTTCAATTTTGCACCATCTAAATTATCAGATATTAACAAAAAAAACGAAACAGTATTTTTTTTAAAAGAAGTTAAAGTAATTAAAAAAAACAATCGTAAGCATTATAAACAAACTCTTTTTGATAAATTAAAAATGAATAAGGCACTAATATTAACAATTCCTTATTTTTTAGTAACTATTTTTTTATTGATAATACCATTGGTTAGTATTATTGTTAAAACTTTTATTAACAATACTGATTCAACTGGTGTAACAACAAACATCATTGATAATTGGTCAGTAATTACGCCAACTATTGGAATGAAAATTTTTAATTCGTTATGA", "aa": "MNKQFNFNFAPSKLSDINKKNETVFFLKEVKVIKKNNRKHYKQTLFDKLKMNKALILTIPYFLVTIFLLIIPLVSIIVKTFINNTDSTGVTTNIIDNWSVITPTIGMKIFNSL", "aa_hexdigest": "45bb987cf420c488ab3301b519e21941", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13084.448399999983, "isoelectric_point": 10.05012149810791 }, "id": "GPKLONFDEH_264", "locus": "GCA000006625_00224" }, { "type": "cds", "sequence": "contig_1", "start": 138805, "stop": 139341, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR36", "UniRef:UniRef90_UPI0026F2788B" ], "nt": "ATGTTAGTAAAACTAATTGGTATTAAAACCTTTTTTGATGTAGTAAATGGAGAAATTAATAGTACACATGGGCACATTTTTACTATTTTAGGTTTAGTTTATATTAATTTGCCATTAATGATTTTGCCTATTTATACATCTTTGTCAACAATGCCTAAAAACCTAGTTAATGCTAGTTATGATTTAGGAAGAGGTTTTTTTTACACCTTCTTTCATATTGTAATTCCTTACACTAAAAATGCTTTAATTTCAGGAGTTTGGATTGTTTATTTAGCAGCATTTACATCAGTTGTGGTTTCAGGTTTTTTAAATAATGATAACGCTGGAGGACTAATTGGTGGAGAAATCTTTGCTCAAGGACAAGACGGTATTTCAAGTGGAGTTCAACTATCACGGTCATCAACAATTACTTTAGTAATTAGTTTAATTTTATTAGGGATTTATCTTATTATTGTTATTATTCCAAAAATAGTCCAATTAATTTATTACAAACGAAAAAATCTAAAAAAAGGAGTAAAGAATAATGAAGCAGATTAA", "aa": "MLVKLIGIKTFFDVVNGEINSTHGHIFTILGLVYINLPLMILPIYTSLSTMPKNLVNASYDLGRGFFYTFFHIVIPYTKNALISGVWIVYLAAFTSVVVSGFLNNDNAGGLIGGEIFAQGQDGISSGVQLSRSSTITLVISLILLGIYLIIVIIPKIVQLIYYKRKNLKKGVKNNEAD", "aa_hexdigest": "67eececb84801828bc3668369699cb98", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 138505, "stop": 139341, "observations": { "mutation": [ 138673 ], "stop": [ 138673 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0026F2788B", "query_cov": 1.0, "subject_cov": 0.5313432835820896, "identity": 0.978, "score": 330.0, "evalue": 8.23e-112, "gene_start": 1, "gene_end": 178, "reference_start": 158, "reference_end": 335, "reference_sequence": "MNKQFNFNFAPSKSSDINKKNETVFFLKEVKVIKKNNRKHYKQTLFDKLKMNKALILTIPYFLVTIFLLIIPLVSIIVKTFINNTDSTGVTTNIIDNWSVITPTIGMKIFNSLWIAAVATIFCLIIGYPFAYFLSIGKSKFLKAIAIALITSPIWMSMLVKLIGIKTFFDVVNGEINSTHGHIFTILGLVYINLPLMILPIYTSLSTMPKNLVNASYDLGRGFFYTFFHIVIPYTKNALISGVWIVYLAAFTSVVVSGFLNNDNAGGLIGGEIFAQGQDGISSGVQLSRSSTITLVISLILLGIYLIIVIIPRVVQLIYYKRKNLKKGAKNNETD" }, "paralog": false, "identity": 0.9820143884892086, "score": 533.0, "evalue": 2.74e-194, "description": "Internal stop codon at 138673. Nonsense mutation around 138673.", "psc": { "uniref90_id": "UniRef90_UPI0026F2788B", "product": "ABC transporter permease", "uniref50_id": "UniRef50_Q9PR36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0026F2788B", "UniRef:UniRef50_Q9PR36" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR36" ], "product": "Spermidine/putrescine transport system permease" } }, "id": "GPKLONFDEH_265", "locus": "GCA000006625_00225" }, { "type": "cds", "sequence": "contig_1", "start": 139634, "stop": 140041, "strand": "+", "frame": 2, "gene": "potC", "product": "Spermidine/putrescine transport system permease", "db_xrefs": [ "COG:COG1177", "COG:E", "SO:0001217", "UniRef:UniRef50_Q9PR35", "UniRef:UniRef90_Q9PR35" ], "nt": "ATGACAGCATCATCACAAACAAACATAATGATTCCAGACGTAATTAGTGGTATTTCATTATCTTTATTATTTGCTGCTACTTTTATTCCAATTGGTTTTAGTTTTGGATTTGGTACGATTATATTAGCACACATTTCTTCTTGCACTCCTTATGCAATTATGATTATTTATCCTAGAATGTTAAAAATGAAAAAAAATTTAATTCTTGCTAGTTATGATTTAGGTTATACAAAAATTGCAACCTTTTTTAGAATTATTTTACCTTATTTATTACCTTCAATTATTTCAGCAACAATCATTGTTTTTGCTATGTCTTTTGATGATTTTATTATTACTAAATTGGTTGGAGGTAAAGTTAATACCATTGGAACAGAAATGTATTCGATGGCTAAAGGAATTAAAGCATGA", "aa": "MTASSQTNIMIPDVISGISLSLLFAATFIPIGFSFGFGTIILAHISSCTPYAIMIIYPRMLKMKKNLILASYDLGYTKIATFFRIILPYLLPSIISATIIVFAMSFDDFIITKLVGGKVNTIGTEMYSMAKGIKA", "aa_hexdigest": "47dc4da9900c1b63ec45d79783f866d0", "start_type": "ATG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "both", "pseudogene": { "start": 139337, "stop": 140182, "observations": { "mutation": [ 139343, 139352, 139373, 140039, 140165 ], "stop": [ 139343, 139352, 139373, 140039, 140165 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR35", "query_cov": 1.0, "subject_cov": 0.4753521126760563, "identity": 0.956, "score": 239.0, "evalue": 1.34e-77, "gene_start": 1, "gene_end": 135, "reference_start": 103, "reference_end": 237, "reference_sequence": "MNNINWLSWLRNSYIWIVLAIIYIPLIIIVLLSFTTPSIKGNITSAFDWNDGSNYLTLTTNQFTDALVNTIIVSIIAVPISTIIATMTCFGVWHAKAFYKKLMTASAQTNMMIPDVISGISLALLFAATFIPIGFSFGFSTIILAHISYCTPYAIMIIYPRMLKMKKNLILASYDLGYTKIATFFKIILPYLLPSIISATIIVFAMSFDDFIITKLVGGKVNTIGTEMYSMAKGIKAWAVCFGALMVLLTILIAALISANKIIKLKLINKQTNTRKLKIWNKQV" }, "paralog": false, "identity": 0.9252669039145908, "score": 499.0, "evalue": 2.83e-182, "description": "Internal stop codon at 139343, 139352, 139373, 140039, 140165. Nonsense mutation around 139343, 139352, 139373, 140039, 140165.", "psc": { "uniref90_id": "UniRef90_Q9PR35", "gene": "PotC", "product": "Spermidine/putrescine transport system permease", "uniref50_id": "UniRef50_Q9PR35", "cog_id": "COG1177", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR35", "UniRef:UniRef50_Q9PR35", "COG:COG1177", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR35" ], "product": "Spermidine/putrescine transport system permease" } }, "id": "GPKLONFDEH_266", "locus": "GCA000006625_00226" }, { "type": "cds", "sequence": "contig_1", "start": 140063, "stop": 140167, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATTAACAATTTTAATTGCTGCATTAATTAGTGCTAATAAAATTATTAAGTTAAAATTAATTAATAAACAAACTAATAAACGCAAATTAAAAATTTGA", "aa": "MVLLTILIAALISANKIIKLKLINKQTNKRKLKI", "aa_hexdigest": "fcdcc9f3f82b7a1140ced7bbdcd30057", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3858.8963000000012, "isoelectric_point": 11.471713829040528 }, "id": "GPKLONFDEH_267", "locus": "GCA000006625_00227" }, { "type": "cds", "sequence": "contig_1", "start": 140186, "stop": 140494, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAATAAAAAATTATTAAAAATTTTTGCTGGAATTACTAGTTTGTTAGTTTTTATACCTATAATTACAGCTTGTAGTCAAAAATCAAAACTTGTAGTTGGTAATTTTGATTCTTATATGGATCCAGATGTTGCTAATGAGCAAGCAAAACGATTTCGTAAAAATAATTTATCATATAGTTTTTATGATAATAATGAAATTTTAAGTTCATTAATTAAAGCAAAAATTTCAGATGTACAAGTTGCTTCAGCATATGAAGTAGCTAAATTAGCAAAAAATGGATTAATTAAGAAAATTAATTGA", "aa": "MKINKKLLKIFAGITSLLVFIPIITACSQKSKLVVGNFDSYMDPDVANEQAKRFRKNNLSYSFYDNNEILSSLIKAKISDVQVASAYEVAKLAKNGLIKKIN", "aa_hexdigest": "e738aaf252cbdacf2338c5536f05e237", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11401.27839999999, "isoelectric_point": 9.715466117858885 }, "id": "GPKLONFDEH_268", "locus": "GCA000006625_00228" }, { "type": "cds", "sequence": "contig_1", "start": 140654, "stop": 140743, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCCTTATTTTTACCAAGATTTAATTTTTGCTTATCGTGGTAAAAAAATTCCTAATTTGGATGATAATAAAAAAGATGTTTATTGA", "aa": "MIPYFYQDLIFAYRGKKIPNLDDNKKDVY", "aa_hexdigest": "62c019cc97f47f991068c25f1ccff54f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3569.0892000000013, "isoelectric_point": 8.03239002227783 }, "id": "GPKLONFDEH_269", "locus": "GCA000006625_00229" }, { "type": "cds", "sequence": "contig_1", "start": 140921, "stop": 142222, "strand": "+", "frame": 2, "gene": "potD", "product": "Lipoprotein", "db_xrefs": [ "COG:COG0687", "COG:E", "SO:0001217", "UniRef:UniRef50_A0AAC9T0J9", "UniRef:UniRef90_A0AAC9T0J9" ], "nt": "ATGCAATTAGAAGGGGATAATAAAAATAACATTATTAAAATTATTAATCATGAAATTCTAGGAATTGATAATAGTATTAGTCAAATTTTACATATTTTAAAAAATCCGAATATAAAAGAAGATATTAAAGAAGAAAATATTAAAAAATTAAAGCGTTTGCAAAAACAATTAGAAGATAGACAAAAAGAATTAAAAACGAATGTGTTCTTACCAGATAGTAGTAGTGTATCTTATATTGAAGATAAATTAAACAATATTAGTAATATGTTTAAAAATCTACATCCTAACTCTATGTATTTAAAAGCAAATTCAAACGATGTATTAAATGATTTAGCAATGGCTAATGTTGCTGGAGCAATTGCTTATAGTGGCGATATTGCTTTTGCTGCTAATGGGGGCGAGTATACAACTGAAGCTGATTCAAATTATGCAAACATGAAGCCAACATCAGATAATTTTCATGTTGTACGACCTAAAGATACGATGAGTGTTTTAGATGGTTTTACAATTAGTAACTCAATTAATAAACAAAACGAACAAGCAGCCTATGAATATTTAAATGAATTATGTTTTGCTGGTCTTAATGAAAAAAACACAGATGGAGAATCTAAAATTTTAGACTATGGTTTGAAAATTCATCGTAATAAAACACCAAAAGAAATTGCTGATTACGAAAATAGTTTAGATGATCAAAATGAAGTTGGTGACGTTTTAGAAGATAGTGGATATTTGTATACGCCAATGCGTAATTTTGATTATGTTCAATATTCGCCAACACTAAATACAATTGCTGATTATGTTTTAGATGAAAACAATGGATTTTATGGAAAAACTGGTTTTGAAAGTGATTTATTAAGACAAAAAATGGTTGAAATCTTTGAAATTGATACAAAATATAAAACAAAAAAAGAGCGCCAACGTAAACATAAATATATTTCATTATTAAATGAAATTATTAATCACATTTATAATACTTATCAAAAAGATAAAAAATTTATTTTTAATCCTACGTTAATTAATGATATATACGAACAAAAACCGATTAAAAATTTAATATATGAGTGTTTAAATAATTTAAATATTAAAATTGAATCACATGTTTTTAATAATTATCTTTTATTAAAATTAGAAGCTATTTTTAATAACATCAATGAAGAGGTTGAAGAACACGCTGCTTTAAACGCGACATTAAGAAAAATTTTTAATGTTCGTTTAAATATTAATACTTTAGAATTTCCCACAAATGATTTATCAATTTCTAATTTAAAATTAGCATACCTTGCCTTCCGTGAAAAGATTTAA", "aa": "MQLEGDNKNNIIKIINHEILGIDNSISQILHILKNPNIKEDIKEENIKKLKRLQKQLEDRQKELKTNVFLPDSSSVSYIEDKLNNISNMFKNLHPNSMYLKANSNDVLNDLAMANVAGAIAYSGDIAFAANGGEYTTEADSNYANMKPTSDNFHVVRPKDTMSVLDGFTISNSINKQNEQAAYEYLNELCFAGLNEKNTDGESKILDYGLKIHRNKTPKEIADYENSLDDQNEVGDVLEDSGYLYTPMRNFDYVQYSPTLNTIADYVLDENNGFYGKTGFESDLLRQKMVEIFEIDTKYKTKKERQRKHKYISLLNEIINHIYNTYQKDKKFIFNPTLINDIYEQKPIKNLIYECLNNLNIKIESHVFNNYLLLKLEAIFNNINEEVEEHAALNATLRKIFNVRLNINTLEFPTNDLSISNLKLAYLAFREKI", "aa_hexdigest": "3a870d1ff3f7ab3d864999fa31ac5680", "start_type": "ATG", "rbs_motif": null, "genes": [ "potD" ], "truncated": "5-prime", "pseudogene": { "start": 140621, "stop": 142222, "observations": { "mutation": [ 140741 ], "stop": [ 140741 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0J9", "query_cov": 1.0, "subject_cov": 0.6386430678466076, "identity": 0.993, "score": 790.0, "evalue": 1.09e-283, "gene_start": 1, "gene_end": 433, "reference_start": 246, "reference_end": 678, "reference_sequence": "MKINKKLLKIFAGITSLLVFIPIITACSQKSKLVVGNFDSYMDPDVANEQAKRFRKNNLSYSFYDNNEILSSLIKAKISDVQVASAYEVAKLAKNGLIKKINWAKFNLKDENNQTITSIEGLKKIFTKQVWAISNAYSSYLGDLDKDGKNDQLLEYMIPYFYQDLIFAYRGKKIPNLDDNKKDVYWSDVFKELTRNDGTPNRFLYEQKANLGFGVDKRTPITIAKNKTKIIAIDDARTIYDIAKIMQLEGDNKNNIIKIINHEILGINNSISQILHILKNPNIKEDIKEENIKKLKRLQKQLEDKQKELKTNVFLPDSSSVSYIEDKLNNISNMFKNLHPNSMYLKANSNDVLNDLAMANVAGAIAYSGDIAFAANGGEYTTEPDSNYANMKPTSDNFHVVRPKDTMSVLDGFTISNSINKQNEQAAYEYLNELCFAGLNEKNTDGESKILDYGLKIHRNKTPKEIADYENSLDDQNEVGDVLEDSGYLYTPMRNFDYVQYSPTLNTIADYVLDENNGFYGKTGFESDLLRQKMVEIFEIDTKYKTKKERQRKHKYISLLNEIINHIYNTYQKDKKFIFNPTLINDIYEQKPIKNLIYECLNNLNIKIESHVFNNYLLLKLEAIFNNINEEVEEHAALNATLRKIFNVRLNINTLEFPTNDLSISNLKLAYLAFREKI" }, "paralog": false, "identity": 0.9924953095684803, "score": 1013.0, "evalue": 0.0, "description": "Internal stop codon at 140741. Nonsense mutation around 140741.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0J9", "gene": "PotD", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T0J9", "cog_id": "COG0687", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0J9", "UniRef:UniRef50_A0AAC9T0J9", "COG:COG0687", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0J9" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_270", "locus": "GCA000006625_00230" }, { "type": "cds", "sequence": "contig_1", "start": 142320, "stop": 142574, "strand": "+", "frame": 3, "gene": null, "product": "putative protein UU111", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR33", "UniRef:UniRef90_Q9PR33" ], "nt": "ATGAATTATAAATATAGTGCTGAAGAAAAACAAATTATTTATAACTATATTTTAAGAGAATATGGGCAAGTGGACCACATTATATTTTTATCAAATGAACATATACGTGTTCCGATTGAATATGATATTTTAGTTATTAAAAAACAAAACTTACAAATATTAATGACTTTTGGACTAGGTGCTTTTAAATCACATAATCACGAAGAAAATACGCAAGAGCGTGCTGAGATTTTTTTAGAATTACCTGTTGATTGA", "aa": "MNYKYSAEEKQIIYNYILREYGQVDHIIFLSNEHIRVPIEYDILVIKKQNLQILMTFGLGAFKSHNHEENTQERAEIFLELPVD", "aa_hexdigest": "eb03bafc78c4c5679b6d1b81808f308c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 142320, "stop": 142877, "observations": { "mutation": [ 142572, 142599, 142662, 142734 ], "stop": [ 142572, 142599, 142662, 142734 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR33", "query_cov": 1.0, "subject_cov": 0.42, "identity": 1.0, "score": 171.0, "evalue": 1.82e-52, "gene_start": 1, "gene_end": 84, "reference_start": 1, "reference_end": 84, "reference_sequence": "MNYKYSAEEKQIIYNYILREYGQVDHIIFLSNEHIRVPIEYDILVIKKQNLQILMTFGLGAFKSHNHEENTQERAEIFLELPVDWDFNKHENMWPVHFLINIVKYSYSNHLTLKWLQTFINPSYFNKSNKIAGFLDLSWYGENSLECKINNDFFVSFYQILIIDDEELFYAKTNGIRALSKFFDDGKSRIVDLNRKSFVK" }, "paralog": false, "identity": 0.9783783783783784, "score": 363.0, "evalue": 7.5e-131, "description": "Internal stop codon at 142572, 142599, 142662, 142734. Nonsense mutation around 142572, 142599, 142662, 142734.", "psc": { "uniref90_id": "UniRef90_Q9PR33", "product": "Uncharacterized protein UU111", "uniref50_id": "UniRef50_Q9PR33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR33", "UniRef:UniRef50_Q9PR33" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR33" ], "product": "Uncharacterized protein UU111" } }, "id": "GPKLONFDEH_271", "locus": "GCA000006625_00231" }, { "type": "cds", "sequence": "contig_1", "start": 142985, "stop": 144112, "strand": "+", "frame": 2, "gene": "dnaQ", "product": "putative protein UU112", "db_xrefs": [ "COG:COG0847", "COG:L", "GO:0003676", "GO:0004527", "GO:0006259", "RefSeq:WP_006689147.1", "SO:0001217", "UniParc:UPI00001392AB", "UniRef:UniRef100_Q9PR32", "UniRef:UniRef50_Q9PR32", "UniRef:UniRef90_Q9PR32" ], "nt": "ATGAAAATATGTGATAATATTAGTGAAATAAATGATGATCAGGATTTAGTCTTTATTGATATTGAAGCAACAGATAGTAAGGGCGAACAACGAATTATTCAGTTTAGCGGATACCGTTTAAATATTAAAAAAAATAAAATTTTTAATTTTAATAAAAAATTCAATCCTGAACAAGAAATTAATTCACGTATTAAAACCTTGCTGAACTTTAATAAAAATTTTAATAATATTGAACAAATGCCAAAATTAGATAAGCAAGCAGCAAGAGAAATTTATTGTTTTGTTAAAAATGCGATTGTCATTACTTTCACAGATTTTGATATTAAAAAAATGCACGAGCTTTTTACATATTATAATTTCGATTTTGAAAAAATCGTTTATTTTGATGTTTATAAGTTTTTTGAAAAAAAATTACAAACTAAATCAGTTCCTAGTTTATTTTCATTAGGAATTCTAAGTGGAATTAAAATTAATTTTTTCAAATTACATAATGCGCTATATGATGCTTTTATTTTAAAAGAAATTTTTATGTGTATTCGTCATAAAACGAACGAAGAATTATATGAAATGTATCGTTATTATGAATTTTTGCCAAAAATTATTTCTTCTTCATATTTTGTTACTAATGAACAAGAAAAAAACAGGGGAATTATTAAAAAAGAAATTAAGTATGTAATGTATATTAAAGAATTTGATTTCTCAAATAAATTTAATTTAGACTTTGTTGTTTATAAAAAAGATCATCATTTTTATTCAAAACCAATTTATGATAGTAGTTTAGAATTGCAAACAATTTCTTCACTAACTTCCAAATCTAATTTTATGAAAGTTAAATTAGCAAACATCTTTTTTAATTATTTAAGTAAAAGTGCTGTTTTTAGTATAAAAAAACTATCAATAAAACAAAGTGAAAAATTCTTAAAATTTTATAAAACACAGACTAATAAAAGAAAGGTTATTAAAGTTTTAAATTTAAATCTTAAAAAAGAAATTAAACCTGAGAATTTTGTCATAAAAGCTCAAATAATTTGTGAAATACTTTTTAAAAACCCAATAATTCATCATTTTGTACATGAATATTTAAAAATTTTTCAAAATATATTTTCTCAAGAAGAAAAAGATAATTAA", "aa": "MKICDNISEINDDQDLVFIDIEATDSKGEQRIIQFSGYRLNIKKNKIFNFNKKFNPEQEINSRIKTLLNFNKNFNNIEQMPKLDKQAAREIYCFVKNAIVITFTDFDIKKMHELFTYYNFDFEKIVYFDVYKFFEKKLQTKSVPSLFSLGILSGIKINFFKLHNALYDAFILKEIFMCIRHKTNEELYEMYRYYEFLPKIISSSYFVTNEQEKNRGIIKKEIKYVMYIKEFDFSNKFNLDFVVYKKDHHFYSKPIYDSSLELQTISSLTSKSNFMKVKLANIFFNYLSKSAVFSIKKLSIKQSEKFLKFYKTQTNKRKVIKVLNLNLKKEIKPENFVIKAQIICEILFKNPIIHHFVHEYLKIFQNIFSQEEKDN", "aa_hexdigest": "c6fe05234d3446a20aa043ecf2091666", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00001392AB", "ncbi_nrp_id": "WP_006689147.1", "uniref100_id": "UniRef100_Q9PR32", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001392AB", "RefSeq:WP_006689147.1", "UniRef:UniRef100_Q9PR32" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR32", "uniref90_id": "UniRef90_Q9PR32" }, "psc": { "uniref90_id": "UniRef90_Q9PR32", "gene": "DnaQ", "product": "Uncharacterized protein UU112", "uniref50_id": "UniRef50_Q9PR32", "cog_id": "COG0847", "cog_category": "L", "go_ids": [ "GO:0004527", "GO:0003676", "GO:0006259" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR32" ], "product": "Uncharacterized protein UU112" }, "genes": [ "dnaQ" ], "id": "GPKLONFDEH_272", "locus": "GCA000006625_00232" }, { "type": "cds", "sequence": "contig_1", "start": 144136, "stop": 145071, "strand": "+", "frame": 1, "gene": "rluA", "product": "Pseudouridine synthase (Uracil hydrolase)", "db_xrefs": [ "COG:COG0564", "COG:J", "SO:0001217", "UniRef:UniRef50_Q9PR31", "UniRef:UniRef90_Q9PR31" ], "nt": "ATGAATTTAAAAGAAATTAAAATTAAAATTAACGATGCCAATCAACGTTTAGATCGTTTTTTATTAAAAAATTTTCCTAATTTATCAAGAATTGCTGTTTATAAAATAATTAGAACAAAAGATGTTAAAGTTAATCATAAGAAAGTTGATCATAATTATTTATTAAAAGAAAATGATGTTGTTAGTATTTATGCTAAGGAGAATTTTTTAGAAAAAAAAGTTGACTTATCTTTTGCTAATGCTAAAGATGAATTAGATATTATATATGAAGATAAAAATATTTTAGTTGTTCACAAACCGATTGGTTTAATTGTTCATGAAGATAATAGATATAAAAATGACACTTTACAAAATAGAATTAAGAAATATTTAGTTAAAAAGGATGAATATCATCCTTTTGATGAAAGTGCTTTTGTTCCTAGCTTATGTCATCGTTTAGATTTAAATACAAGTGGATTAATTGTTGCAGCTAAAACTGCAAATGCTTTAAGAGTAATTACTGAAATGTTTAAAAATAATGATGTGATTCGAATTTATAAATGTTTAACATACAATCAATTACCTAAAAATAATGATGTTATTTATAATTTCATGTATAAAGCTGATGATAACACAATGATTGTACAAAATTTTAAAACAAAATTTAATAAAACAGCAATTACCAAATATACATATATAGATAAGTATAAAAAATATTTTATTTATGACATTGAACTATTAACTGGAAGAACCCACCAAATACGAGCAGTTTTAAATTTTTTGCATGCACCAATTGTTGGTGAACAAAAATACATCACTAAAGATATTGATAAGAATCAAAATTATATGTATCAATGTTTAGTATCATATAAGATCAAATTTATTAATTTAAAGAAATATCAATGTGAAGAATTAAATTATTTAGAGAATAAAGAATTTAGAATAAAAAATATTTGA", "aa": "MNLKEIKIKINDANQRLDRFLLKNFPNLSRIAVYKIIRTKDVKVNHKKVDHNYLLKENDVVSIYAKENFLEKKVDLSFANAKDELDIIYEDKNILVVHKPIGLIVHEDNRYKNDTLQNRIKKYLVKKDEYHPFDESAFVPSLCHRLDLNTSGLIVAAKTANALRVITEMFKNNDVIRIYKCLTYNQLPKNNDVIYNFMYKADDNTMIVQNFKTKFNKTAITKYTYIDKYKKYFIYDIELLTGRTHQIRAVLNFLHAPIVGEQKYITKDIDKNQNYMYQCLVSYKIKFINLKKYQCEELNYLENKEFRIKNI", "aa_hexdigest": "d8a0318b1adbe586f34fce95c6092cb9", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_Q9PR31", "query_cov": 1.0, "subject_cov": 0.9873015873015873, "identity": 1.0, "score": 619.0, "evalue": 7.74e-224, "valid": true, "gene": "RluA", "product": "Pseudouridine synthase (Uracil hydrolase)", "uniref50_id": "UniRef50_Q9PR31", "cog_id": "COG0564", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_Q9PR31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR31" ], "product": "Pseudouridine synthase (Uracil hydrolase)" }, "genes": [ "rluA" ], "id": "GPKLONFDEH_273", "locus": "GCA000006625_00233" }, { "type": "rRNA", "sequence": "contig_1", "start": 145339, "stop": 146855, "strand": "+", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9895629484670581, "score": 1449.2, "evalue": 1.6e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "ATTTTAAAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAATCGAACGAAGCCTTTTAGGCTTAGTGGTGAACGGGTGAGTAACACGTATCCAATCTACCCTTAAGTTGGGGATAACTAGTCGAAAGATTAGCTAATACCGAATAATAACATCAATATCGCATGAGAAGATGTAGAAAGTCGCTCTTTGTGGCGACGCTTTTGGATGAGGGTGCGACGTATCAGATAGTTGGTGAGGTAATGGCTCACCAAGTCAATGACGCGTAGCTGTACTGAGAGGTAGAACAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGGGCGCAAGCCTTATGAAGCAATGCCGCGTGAACGATGAAGGTCTTATAGATTGTAAAGTTCTTTTATATGGGAAGAAACGCTAAGATAGGAAATGATTTTAGTTTGACTGTACCATTTGAATAAGTATCGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAACGAGCGCAGGCGGGTTTGTAAGTTTGGTATTAAATCTAGATGCTTAACGTCTAGCTGTATCAAAAACTGTAAACCTAGAGTGTAGTAGGGAGTTGGGGAACTCCATGTGGAGCGGTAAAATGCGTAGATATATGGAAGAACACCGGTGGCGAAGGCGCCAACTTGGACTATCACTGACGCTTAGGCTCGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATCATCATTAAATGTCGGCCCGAATGGGTCGGTGTTGTAGCTAACGCATTAAATGATGTGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTTGACAATACACGTAGAACCTTACCTAGGTTTGACATCTATTGCGATGCTATAGAAATATAGTTGAGGTTAACAATATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTTTCGTTAGTTACTTTTCTAGCGATACTGCTACCGCAAGGTAGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATATCTAGGGCTGCAAACGTGCTACAATGGCTAATACAAACTGCTGCAAAATCGTAAGATGAAGCGAAACAGAAAAAGTTAGTCTCAGTTCGGATAGAGGGCTGCAATTCGTCCTCTTGAAGTTGGAATCACTAGTAATCGCGAATCAGACATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGGGAGCTGGTAATATCTAAAACCGCAAAGCTAACCTTTTGGAGGCATGCGTCTAGGGTAGGATCGGTGACTGGAGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "GPKLONFDEH_33", "locus": "GCA000006625_00234" }, { "type": "rRNA", "sequence": "contig_1", "start": 147148, "stop": 150058, "strand": "+", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9952136752136752, "score": 2802.8, "evalue": 3.9e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TAATAAGTTACTAAGAGCTTATGGTGAATGCCTTGGGACAAACAGGCGATGAAGGACGTGCTAATCTGCGATAAGCAACGGGTAGCTGATAAGAGGCTTTAATCCGTTGATCTCCGAATGAGGAAACTCAATCTAATGAGAATTAGATTACTGTATAAATGAATTCATAGTTTAACAGAGCGATACCTGGTGAAGTGAAACATCTCAGTAACCAGAGGAAAAGAAAACGAAGTGATTCCCTGTGTAGCGGCGAGCGAAAGGGGAGTAGGCCAAACCGAATTTCGATTCGGGGTTGTAGGACTACAATATGGAATAGAGAATGATAGTAGAATTGGTTGGGAAGCCAAATCATAGAGGGTGATAATCCCGTATACGAAATCATTCTTTTACCTAGTAGGATCCTGAGTAGGGCGGGACACGTGAAATCCTGTCTGAATCCACCCAGACCATTGGGTAAGCCTAAATACTAGTTTGTCACCGATAGAGCATAGTACCGTGAGGGAACGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGAACCTGAAACCATAAGCTTACAAGGTGTTAGAGCACATTAATGTGTGATAGCGTGCCTTTTGAAGTATGAGCCAGCGAGTTATTATAGCATGCGAGGTTAAATCGTAGAGATGGAGCCGTAGGGAAACCGAGTCTTAATAGGGCGTTTTAGTATGTTATAATAGACGCGAAACGGGGTGATCTATCCATGGGCAGGTTGAAGGTGAAGTAACATTCACTGGAGGACCGAACCCACTTTCGTTGAAACGACAGGGGATGACCTGTGGATAGTGGTGAAATTCCAATCGAACTCCGTGATAGCTCGTTCTCGTCGAAATATTTTTAGGAATAGCGTTAGATTATTGGGAATATGGGGGTAAAGCACTGAATCTATGATGGCGCCACCTCGGTGTACTGAATAGAATCAAACTCTGAATACCATATTACCTATTCTAGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCGCGAGGGAAACAGCCCAGATCATTAACTAAGGTCCCTAATATATGCTAAGTGGAAAACGATGTTGAGTTTCATAAACAGCAAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGAGACTCTGCGCGGAAGATGTAACGGGGCTAAGCATATAACCGAAGTTATGGGTTACATATTATATATGTAGCGGTAGACGAGTGTTGTATATGGGGCGAAGGTAGACTGTGAAGACTACTGGACTTTATACAAGTAAGAATGCTGGCGTGAGTAACGAATGAGAGTGAGAATCTCTCAAACCGATTGACTAAGGGTTCCTGGGCAAGGGTCGTCCTCCCAGGGTAAGTCGGATCCTAAGGCGAGGCTGAAAAGCGTAGTCGATGGAAAACAGGTTAATATTCCTGTACCTACAAATTGTGTGATGGAGTGACGGAGAAGGTTATTATGTGCCGGTTATTGGATTCCGGTTTAAATAACAAGGTTAGTAAGTTGGCAAATCCGCTTACTATTAAGACCAAGTTATGAATACGAGCGACCCCTTCGGGCAGTAGCGAAGACATATACATCATGCTTCCAAGAAAAGCTTCTAGCGTTAAACAATTAGTAGTCCGTACCGAGAACGAACACACGTGGTCAAGGAGAATATCCTAAGGTTAGCGAGTTAACTACAGTTAAGGAACTCTGCAAATTAACCCCGTACGTTAGCAATAAGGGGTGCTCGCTGTAAAAGGTGAGCCGCAGTGAATAGCGAGGGGGGACTGTTTAACAAAAACACAGCTCTATGCTAAGTCGTAAGACGATGTATATGGGGTGACACCTGCCCAATGCTGTAAGGTTAAAGAAGAATGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAATGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCAACTGTAGACTCGGTGAAATCCTGGTGAGGGTGAAGACGCCCTCTTGGCGTGATTGGACGGAAAGACCCCATGAAGCTTTACTGTAGCTTAATATTGGGAAATTTTATTACTTGTAGAGCATAGGTAGGAGACTGTGAAGTATACTCGCTAGGGTATATGGAGTCAACGTTGGAATACTACCCTTGTGATAAGATTTCTCTAACCTGCAGCCATGAATCTGGCTGGGGGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCCAAAAGGTAACGGAGGCGCGCAACGGTACCCTCAGCACGGTTGGAAATCGTGTATAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAAGTCGAACAGGTAGGAAACTAGGTCATAGTGATCCGGTGGCTCAGAATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCTGGGGATAACAGGCTGATAGTGCCCAAGAGTTCATATCGACGGCACTGTTTGGCACCTCGATGTCGACTCATCTCATCCTGGAGCTGAAGCAGGTTCCAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTGTCATGCCCGTAGGAAGATTGAGAAGAGCTGTTCCTAGTACGAGAGGACCGGAATGGACACACCTCTTGTGATCCTGTTGTCGCGCCAGCGGCACTGCAGGGTAGCAACGTGTGGAATAGAGAAACGCTGAAAGCATCTAAGTGTGAAACTAACTTCAAGATTAATCTTCCCTTTCTTAAATGAAGTAAGAATCGTTAAAGACTATAACGTTGATAGGTCGGATGTGTACGCACGGTGACGTGTTTAGCTAACCGATACTAATAATTCGAGGACTTATATAA", "id": "GPKLONFDEH_36", "locus": "GCA000006625_00235" }, { "type": "rRNA", "sequence": "contig_1", "start": 150123, "stop": 150226, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8739495798319328, "score": 82.2, "evalue": 1.1e-13, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TGTAGTGATTATATCAGAGTGGAAATACCTGTTCCCATCCCGAACACAGAAGTCAAGCACTCTAGAGCCGAAAATAGCGCAAGTAAAATAGGTCATCGCTACGC", "id": "GPKLONFDEH_34", "locus": "GCA000006625_00236" }, { "type": "cds", "sequence": "contig_1", "start": 150492, "stop": 150839, "strand": "+", "frame": 3, "gene": "folA", "product": "Dihydrofolate reductase", "db_xrefs": [ "COG:COG0262", "COG:H", "EC:1.5.1.3", "GO:0004146", "GO:0005829", "GO:0006730", "GO:0046452", "GO:0046654", "GO:0046655", "GO:0050661", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_Q9PR30", "UniRef:UniRef90_Q9PR30" ], "nt": "ATGGGTAAAAACACTTTTGATTCATTAGATCAAAAACCATTAAATAATCGAACAAATATCATTTTTTCTTCAATTATGCAAACACCAGAAGACGAATCGTATTTTGTGACAAATGATTTTCAACAAGTATTAAACGATGCTAAAAAAGAGGATATTTTTATTATTGGCGGCAAGGAATTATTTGATATTTTTTTAGCTTATGCAGATGTTTTAATTGTTTCTGTATTAAAAGATTATTATGATTGTGATCTATACATGAAAGTTGATTATAATAATTTTAATTTAGATAAACAAGATGTTTATGATAATTTTATTGTTAATTATTATTCAAATAAGAAGGAAAAATAA", "aa": "MGKNTFDSLDQKPLNNRTNIIFSSIMQTPEDESYFVTNDFQQVLNDAKKEDIFIIGGKELFDIFLAYADVLIVSVLKDYYDCDLYMKVDYNNFNLDKQDVYDNFIVNYYSNKKEK", "aa_hexdigest": "5d0f9ee231a0a6c4e30426b0cf5e5d05", "start_type": "ATG", "rbs_motif": null, "genes": [ "folA" ], "truncated": "5-prime", "pseudogene": { "start": 150369, "stop": 150839, "observations": { "mutation": [ 150384, 150432 ], "stop": [ 150384, 150432 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR30", "query_cov": 1.0, "subject_cov": 0.7371794871794872, "identity": 1.0, "score": 229.0, "evalue": 1.65e-75, "gene_start": 1, "gene_end": 115, "reference_start": 42, "reference_end": 156, "reference_sequence": "MLKLIWCQTLNGGISKNNKLPWYIKEELDHFYKTTKNHKIIMGKNTFDSLDQKPLNNRTNIIFSSIMQTPEDESYFVTNDFQQVLNDAKKEDIFIIGGKELFDIFLAYADVLIVSVLKDYYDCDLYMKVDYNNFNLDKQDVYDNFIVNYYSNKKEK" }, "paralog": false, "identity": 0.9871794871794872, "score": 314.0, "evalue": 1.07e-111, "description": "Internal stop codon at 150384, 150432. Nonsense mutation around 150384, 150432.", "psc": { "uniref90_id": "UniRef90_Q9PR30", "gene": "folA", "product": "Dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_Q9PR30", "cog_id": "COG0262", "cog_category": "H", "kegg_orthology_id": "K00287", "go_ids": [ "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR30", "EC:1.5.1.3", "UniRef:UniRef50_Q9PR30", "COG:COG0262", "COG:H", "KEGG:K00287", "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR30" ], "product": "Dihydrofolate reductase" } }, "id": "GPKLONFDEH_274", "locus": "GCA000006625_00237" }, { "type": "cds", "sequence": "contig_1", "start": 150847, "stop": 151422, "strand": "+", "frame": 1, "gene": null, "product": "Phosphoglycolate phosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR29" ], "nt": "ATGACCAAAAAACTGATTGTTTTTGATTTTGATGGTACAATTATGCACACGCATACAACTATGAGCCTATCTATTATTGGTGTTTTAGAATTTTATAATCATCATATACCAAGTTTAAAAGAAATGAACGATCTGTTAGGTAATCTTTCTATGGTAAATATTTTTAAAAAGTATGCTAAACACGATCTTTTAAATATCGAAATTGAAATGATGATTTCTAAATATTATGAAATATATGAATCTTCGCTTTTTATGATCCATAGTTATTTTTTTGATGGTATTTTAGACCTAATCAAAAAATTAAAATCACTTAATAATAATGTTAAATTAGCAATTTTATCAAATAAACGATCTTCTTTATTAGCAACAATGGTTGATTATTATAATTTACGACCTTATTTTGATTATATTTTTGGTGCTGAAGATGTCGAACAAATGAAGCCAGATCCTAGTGGTTTACTAAAAATTATGAACAACTGTAATGTTGATCATAAAAATACTTTATTGATTGGTGATAGTATAGCTGATTTAAAAGCAGCTATAAATGCTAATTGTCATTTTATTTTAGTTAATTGA", "aa": "MTKKLIVFDFDGTIMHTHTTMSLSIIGVLEFYNHHIPSLKEMNDLLGNLSMVNIFKKYAKHDLLNIEIEMMISKYYEIYESSLFMIHSYFFDGILDLIKKLKSLNNNVKLAILSNKRSSLLATMVDYYNLRPYFDYIFGAEDVEQMKPDPSGLLKIMNNCNVDHKNTLLIGDSIADLKAAINANCHFILVN", "aa_hexdigest": "c3293af79bf3974e8ddfde5fe6522b21", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0AA86YSP4", "query_cov": 1.0, "subject_cov": 0.8488888888888889, "identity": 0.853, "score": 332.0, "evalue": 3.96e-114, "valid": false, "product": "Phosphoglycolate phosphatase", "uniref50_id": "UniRef50_Q9PR29" }, "pscc": { "uniref50_id": "UniRef50_Q9PR29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR29" ], "product": "Phosphoglycolate phosphatase" }, "genes": [], "id": "GPKLONFDEH_275", "locus": "GCA000006625_00238" }, { "type": "tRNA", "sequence": "contig_1", "start": 151584, "stop": 151657, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 72.5, "nt": "GCAGATGTAGTTCAATGGTAGAACGCAACCTTGCCAAGGTCGAGACGCGAGTTCGATTCTCGTCATCTGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 151616, 151618 ], "id": "GPKLONFDEH_1", "locus": "GCA000006625_00239" }, { "type": "cds", "sequence": "contig_1", "start": 151722, "stop": 152345, "strand": "-", "frame": 2, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206", "RefSeq:WP_006688866.1", "SO:0001217", "UniParc:UPI0000137D44", "UniRef:UniRef100_Q9PR28", "UniRef:UniRef50_P70881", "UniRef:UniRef90_Q9PR28" ], "nt": "ATGCATAAAATAATTAATCACCCATTAATTAAAGATAAACTAACACGTATGCGAAAAGTTTCAACAGTTTCTACTGTTTTTCGTACAAATTTAGAAGAACTTACACAATTGATGGTTTATGAAGCAACAAAAGATTTAGAGTTAAATGAAATAGAAATTGAAACTCCTGTTGTTAAGGTTGCAAAAGGTTATAAATTAAAAAATAAAATTTGTTTAATACCTATTTTGCGCGCTGGAATTGGAATGGTTGATGGTGTTAAAAGTTTAATTCCAACAGCCACAATCGGTCATATCGGTTTATATCGTAATGAACAAACACTAAAACCAGTAGAATATTTTAAAAAATTTCCTAAAAATATTTCTGAATCAGATGTTATTATTTTAGATCCAATGTTAGCAACAGGAGGTAGTGTTGTTGAAGCTGTTAATATTATTAAAAAATACAATCCAAAATCCATTAAATTTGTATGTATTGTTGCAGCTCCAGAAGGTCTTAAATATGTTCAAGAAGTTCATCCTGAAGTTGATGTTTATATCGCAGCATTAGATGATAAATTAAATGAAAATGGATATATCACTCCTGGTTTAGGTGATGCTGGTGATCGAATTTTCGGTACTAAATAA", "aa": "MHKIINHPLIKDKLTRMRKVSTVSTVFRTNLEELTQLMVYEATKDLELNEIEIETPVVKVAKGYKLKNKICLIPILRAGIGMVDGVKSLIPTATIGHIGLYRNEQTLKPVEYFKKFPKNISESDVIILDPMLATGGSVVEAVNIIKKYNPKSIKFVCIVAAPEGLKYVQEVHPEVDVYIAALDDKLNENGYITPGLGDAGDRIFGTK", "aa_hexdigest": "d21432270e9d782a14c0a824285ae240", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000137D44", "ncbi_nrp_id": "WP_006688866.1", "uniref100_id": "UniRef100_Q9PR28", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137D44", "RefSeq:WP_006688866.1", "UniRef:UniRef100_Q9PR28" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR28", "uniref90_id": "UniRef90_Q9PR28" }, "psc": { "uniref90_id": "UniRef90_Q9PR28", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_P70881", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_P70881", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P70881" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "GPKLONFDEH_276", "locus": "GCA000006625_00240" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 152467, "stop": 152564, "strand": "-", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 58.4, "evalue": 3.1e-07, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "TATATATGTCATTACAATATTAAGTAGCGAACCTTGTCAGGCCAGAGATGGAGCAGCAATAGCAATATCTTTTTATGTGTGATGACATATATTAATGT", "id": "GPKLONFDEH_40", "locus": "GCA000006625_00241" }, { "type": "cds", "sequence": "contig_1", "start": 152602, "stop": 153837, "strand": "-", "frame": 1, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "db_xrefs": [ "COG:COG0162", "COG:J", "EC:6.1.1.1", "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437", "KEGG:K01866", "SO:0001217", "UniRef:UniRef50_Q9PR27", "UniRef:UniRef90_Q9PR27" ], "nt": "ATGCATAGTTTAATTAAGGATTTAAAAGCACGAAACTTAATCAATAATATAACTAATGAAGAAAAATTAATAAAAGCTTTAGAGAAAAATAAAGGAATTTATGTAGGTTTTGATCCTTCAGCTGATAGTTTACACTTAGGTAATTATATTATGATAATGTTATTAAAACGCTTTCGTTTATATAACATTAAAACATTAGCGCTTGTTGGTGGTGCTACAGGAATGATTGGTGATCCAAGTGGTAAATCCGCTGAACGTAATTTATTAGATAAAACAATTTTAGAAAAAAATATTGCAAAAATAAAATTTCAACTTGAAAAATTTACTAATTCTCAAGTTATTAATAATTATGTTTTTTATGAAAATATGACTTTTTTAGATTTTTTGCGTGATGTTGGAAAGTTAATCAACATTAATTATTTATTAGAAAAAGAAATTATTAATTCTCGTTTATCTGTTGGTATTTCATATACAGAATTTAGTTATAATTTGCTACAAGGTTATGATTTTTTACAATTATATAAAAATGATAACATAGCCATTCAAGCTGGTGGTAGTGATCAATGGGGTAACATTACAACTGGAATTGAAATGATTCGAAAAAATTTAGGTGATGACAATATCGCTTGTGGATTGACAATTAATTTATTAACAAATTCAGAAGGTAAAAAATTTGGTAAAAGTGAAAAAGGTGCTATTTATTTAGACGAAAATAAATCAACTGTTTATGAAATGTATCAGTTTTTAATTAATCAAAGCGATGCTGATGTAGAAAAATTACTTAATTTTTTAACATTAATTGATGTTGAAGAAATAAAAAAAATAATGCAAGCTCATAAAAATAATCCCGCTTTACGAGTTGCACAAAAAGCATTGGCTAAAGCTGTTGTTGTTGATATACATGGACAACAAAAATATGAACAAGCATTACATATTAGCGAAGTACTATTTAATGGTAGTATTAGTACATTAAATCAAGAAGAATTAGAAATCGCAATTAAGAGTTTACCTGCAACAAAATTAGACAAAGATGAAATTAAAATAATTGATTTATTAAATTTAGCTAATATTTCATCAAGTAACCGTATAGCACGAGATTTTTTAAATACAGGATCAATTTTAATTAATGATATTAAAATAAATGATGAAAATTTTTTAGTCAAGAAACAAGATGCTATTAATCAAAAATTTAGTATTATTAGAAAAGGTAAACGAAATTATTTTTTAATTTTATGA", "aa": "MHSLIKDLKARNLINNITNEEKLIKALEKNKGIYVGFDPSADSLHLGNYIMIMLLKRFRLYNIKTLALVGGATGMIGDPSGKSAERNLLDKTILEKNIAKIKFQLEKFTNSQVINNYVFYENMTFLDFLRDVGKLININYLLEKEIINSRLSVGISYTEFSYNLLQGYDFLQLYKNDNIAIQAGGSDQWGNITTGIEMIRKNLGDDNIACGLTINLLTNSEGKKFGKSEKGAIYLDENKSTVYEMYQFLINQSDADVEKLLNFLTLIDVEEIKKIMQAHKNNPALRVAQKALAKAVVVDIHGQQKYEQALHISEVLFNGSISTLNQEELEIAIKSLPATKLDKDEIKIIDLLNLANISSSNRIARDFLNTGSILINDIKINDENFLVKKQDAINQKFSIIRKGKRNYFLIL", "aa_hexdigest": "6d8ada0e833d7e60a0090ef3dea517f5", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q9PR27", "query_cov": 1.0, "subject_cov": 0.9903614457831326, "identity": 1.0, "score": 785.0, "evalue": 1.88e-286, "valid": true, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_Q9PR27", "cog_id": "COG0162", "cog_category": "J", "kegg_orthology_id": "K01866", "go_ids": [ "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR27" ], "product": "Tyrosine--tRNA ligase" }, "genes": [ "tyrS" ], "id": "GPKLONFDEH_277", "locus": "GCA000006625_00242" }, { "type": "cds", "sequence": "contig_1", "start": 153976, "stop": 154866, "strand": "+", "frame": 1, "gene": "lolD", "product": "ABC Transporter", "db_xrefs": [ "COG:COG1136", "COG:M", "SO:0001217", "UniRef:UniRef50_Q9PR26", "UniRef:UniRef90_Q9PR26" ], "nt": "ATGAGAAATAAAAAAGAAAATGATAATGTTATTAATCCATTATCTAATCCAGATGAGAACAAAAAAATTAATATAAAAGACTTATCAAAAGATGAGCGTAAAGAACTTAAAAAACGAATTAAAGAAGTTGAAAAATTAAACAGAAATCACAAAGGTGACATTGATATGCCATCACGTGATCCAAATAATATTATTGAACTTCGTGATGTTAAAAAAATTTTCACAAATGGCTATTTAATTAATGAAACACTCAAAGGTGTTAATTTAGATATTCGTAAAGGTGAATTTGTAATTATTTTAGGACCATCAGGTTCTGGTAAAACAACATTAATGAACATTATGTCGGGACTTGATCGTGCAACTGATGGTGATGTTCGTGTTTGTGATAAACAACTAATTAATATGAATCAAAATAAATTAACTGATTTTCGTAAAGAATATATTGGATTTGTTTTTCAACAATATGGATTATTACCAACACTATCTGTAGAGGAAAATGTAGAGATTGGTGCTGATTTACAAGTTGATAAAAAACGCCGTATTAAACCACAAGATGCATTAAAAGCAGTAGGGATGCTTGATTACGCAAAAAAATTTCCACATGAATTATCTGGTGGTCAGCAACAACGAGTCTCAATAGCCCGTGCTTTAGCTAAAAATCCTATTATTTTATTTGGCGATGAACCAACTGGTGCTGTTGATGAAACAATGTCAAAAATTATTTTAAAAGAATTTGTAAAAGTTAACCAGGAATTAAAGACAACGGTAATTATTGTTACCCACAACCCAATTTTTGCTGAACTTGGAACTTTAGTTATTAAGGTTAAAGATGGTAATATTAATGAATTAATTCGTAATGATCATCCAAAATCAGTTGACGAATTAAAATGA", "aa": "MRNKKENDNVINPLSNPDENKKINIKDLSKDERKELKKRIKEVEKLNRNHKGDIDMPSRDPNNIIELRDVKKIFTNGYLINETLKGVNLDIRKGEFVIILGPSGSGKTTLMNIMSGLDRATDGDVRVCDKQLINMNQNKLTDFRKEYIGFVFQQYGLLPTLSVEENVEIGADLQVDKKRRIKPQDALKAVGMLDYAKKFPHELSGGQQQRVSIARALAKNPIILFGDEPTGAVDETMSKIILKEFVKVNQELKTTVIIVTHNPIFAELGTLVIKVKDGNINELIRNDHPKSVDELK", "aa_hexdigest": "41a624662403dd7d52180f918d8a91ea", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_Q9PR26", "query_cov": 1.0, "subject_cov": 0.9866666666666667, "identity": 0.9590000000000001, "score": 540.0, "evalue": 2.12e-193, "valid": true, "gene": "LolD", "product": "ABC Transporter", "uniref50_id": "UniRef50_Q9PR26", "cog_id": "COG1136", "cog_category": "M" }, "pscc": { "uniref50_id": "UniRef50_Q9PR26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR26" ], "product": "ABC Transporter" }, "genes": [ "lolD" ], "id": "GPKLONFDEH_278", "locus": "GCA000006625_00243" }, { "type": "cds", "sequence": "contig_1", "start": 154895, "stop": 155494, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07517.20" ], "nt": "ATGAATTTAATATCAAAAATATCCCCCCAAAACCGAATCTTAAATCGCGCTCGATTGATTGCTGAAGAAGTTTTAAAAAAAGAAGAAGAATACGAACATTTTAGCGATCAAGAATTAATTAATAAATCTGATGATATTATTGAATATTTAGCAAATAATAACCCATTAGATGATAAATTAGTAGAAGCATTATGCATTATTCGTGAAGTGATTTATCGAGTTCATAATAAAAGAGCTTTTAAAGTTCAAATTATTGGTGCAATTATTGTTTATTTTGGTGATTTTGCTGAAATGATGACTGGTGAGGGTAAAACATTAACTTTAGTTTTAGTTGCTTATTTAAATGCACTTTATAAAAAAGGTGTTCATATGGTAACTGTTAACGAATATCTAGTTAAAGTTGGAGCAGAATTTGCAACACCTGTTTTAAATTTTTTAAATATGTCTGTTGGCCAAATTACAGCTAATATGAATGAGTATGAAAAACGAAATAATTATAATTGTGATATCACATATACAACTAATTCAGAATTAGGCTTTGATTATTTACGTGATAATATGGTAACTAATTATGCTAATAAAGTTCAACGGGGCTTATGA", "aa": "MNLISKISPQNRILNRARLIAEEVLKKEEEYEHFSDQELINKSDDIIEYLANNNPLDDKLVEALCIIREVIYRVHNKRAFKVQIIGAIIVYFGDFAEMMTGEGKTLTLVLVAYLNALYKKGVHMVTVNEYLVKVGAEFATPVLNFLNMSVGQITANMNEYEKRNNYNCDITYTTNSELGFDYLRDNMVTNYANKVQRGL", "aa_hexdigest": "bab72ca190832d102e01c33dc36407f1", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07517.20", "name": "SecA_DEAD", "length": 192, "aa_cov": 0.964824120603015, "hmm_cov": 1.0, "evalue": 1.3610309788655823e-69, "score": 230.1531219482422, "start": 8, "stop": 199 } ], "seq_stats": { "molecular_weight": 22903.04580000001, "isoelectric_point": 5.486577033996581 }, "id": "GPKLONFDEH_279", "locus": "GCA000006625_00244" }, { "type": "cds", "sequence": "contig_1", "start": 155870, "stop": 157060, "strand": "+", "frame": 2, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0006886", "GO:0008564", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002", "SO:0001217", "UniRef:UniRef50_Q9PR25", "UniRef:UniRef90_Q9PR25" ], "nt": "ATGGAAGGAAGAAGTTATAGCGCTGGATTACAACAAGCAATTCAAGCTAAAGAACAAATTAAAATTGAACCCGAAAATTTAACAGTTGCCACAATCACTTATCAATCATTATTTCGTTTATATAAAAAATTAGCAGCAGTTTCAGGAACAGCTATTACTGAAGCAGAAGAATTTTTAAATATTTATAATATGGTTGTTGTTACAATCCCAACAAATAAACCAATTAAAAGAATTGACCATCCTGATTATGTTTTTGATAATAAAAGAACAAAATGGAAATATGTAATTGCTGATGTTATTCGTCGTCATGAAAATGGCCAACCAATTTTAATTGGAACTGCTAGTGTTGAAGATTCAGAAATTTTACATCAATTACTAGAACGAGTCAATATTCCTCACGAAGTTTTAAATGCTAAAAATCACGCACGCGAAGCTGAAATTATTGCTTGTGCAGGAGAGTATAAAGCAGTTACAATAGCAACAAATATGGCAGGCCGTGGGACTGATATTAAATTATCTCCAGAATCATTAGAAGCTGGAGGATTATGTGTTATTGGAACCGAACGAAGTGATTCACGTAGAATTGATAATCAGTTAAGAGGACGTGCTGGACGACAAGGTGATATTGGCGAATCACGTTTTTTTATTTCAATGGAAGATACTTTATTTAGTCGTTTTGCAACCGATAATTTAGCAAAAGCTGATGATAAATTAAGTGAAGATGTTATTTCAACAAAATTCTTTACACGATTGCTAAATAATACACAAAAAAAAGTTGAGTCGTTAAACTATGACACAAGAAAAAATCTAATTGATTATGATCATGTTTTAAGTAATCAACGCGAATTAATTTACAAACAACGGGATAAAATTCTTATTTCATCAGATAATAAAGATATTTTATATCGAATGTTAGATAGTGTAATTGATGATTTAATTTATCAATCACACAATAAACCAAATGAAGACATTATTGACATAAAAAAATTAATTGATTTAGCAACTCAAAACATTTTTTATGACAATTATTTAAATCAGGACGAATATTATGGGCTTAAGTTTGAACAAATTAAAACTAAATTAAAAAAAGACTGTATTAATTTTTTTGAGCAAAAAGAACAACTAATGACACCGACAATTTTTAATCAAATCTTATCAGAAATTATGATTAGTAATATTGATGAAGAATGA", "aa": "MEGRSYSAGLQQAIQAKEQIKIEPENLTVATITYQSLFRLYKKLAAVSGTAITEAEEFLNIYNMVVVTIPTNKPIKRIDHPDYVFDNKRTKWKYVIADVIRRHENGQPILIGTASVEDSEILHQLLERVNIPHEVLNAKNHAREAEIIACAGEYKAVTIATNMAGRGTDIKLSPESLEAGGLCVIGTERSDSRRIDNQLRGRAGRQGDIGESRFFISMEDTLFSRFATDNLAKADDKLSEDVISTKFFTRLLNNTQKKVESLNYDTRKNLIDYDHVLSNQRELIYKQRDKILISSDNKDILYRMLDSVIDDLIYQSHNKPNEDIIDIKKLIDLATQNIFYDNYLNQDEYYGLKFEQIKTKLKKDCINFFEQKEQLMTPTIFNQILSEIMISNIDEE", "aa_hexdigest": "8313da75dcca15d557a63992a47a03da", "start_type": "ATG", "rbs_motif": null, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 155570, "stop": 157363, "observations": { "mutation": [ 157058, 157193 ], "stop": [ 157058, 157193 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR25", "query_cov": 1.0025252525252526, "subject_cov": 0.47205707491082044, "identity": 0.9570000000000001, "score": 740.0, "evalue": 3.87e-262, "gene_start": 1, "gene_end": 396, "reference_start": 326, "reference_end": 722, "reference_sequence": "MNLISKISPQNRILNHARLIAEEVLKKEDEYTHFSDQELINKSDDIIEYLANNNPLDDRLVESLCIIREVIYRVHNKRAFKVQLIGAIIVYFGDFAEMMTGEGKTLTLVLVAYLNALYKKGVHMVTVNEYLVKVGAEFATPALNFLNMSVGQITANMNEYEKRNNYNCDITYTTNSELGFDYLRDNMVTNYNSKVQRGLWFAIVDEGDSVLIDEARTPLIISGEPQEEIGNYVKADRFVKTLYPQDFTLDPESQSVALTESGVEKAQKFFNTKNYYNFENSDIIHKVTNALRANFTFFNGREYIVKKDDEGEDVIALVDQSTGRIMEGRSYSAGLQQAIQAKEQIKIEPENLTVATITYQSLFRLYKKLAAVSGTAITEVEEFLNIYNMVVVTIPTNKPIRRIDHPDYVFDNKRTKWKYVIADVIRRHENGQPILIGTASVEDSEILHQLLERVNIPHEVLNAKNHAREAEIVARAGEYKAVTIATNMAGRGTDIKLSPESLEAGGLCVIGTERSDSRRIDNQLRGRAGRQGDIGESRFFISMEDTLFSRFATDNLAKADDKLSEDVISTKFFTRLLNNTQKKVESLNYDTRKNLIDYDHVLSNQRELIYKQRDKILVSSDNKDILYRMLDSVIDDIIYQSHNEPNEDIIDVKKLIDLATQNIFYDNYLNHDEYYGLDLIDEIKTKLKNDCISFFEQKEQLMTPGIFNQILSEIMISNIDEEWTKHLDVTSKIREGVNLRAYEQKAPLNIYVEDSDKLFEKLKHDVAWKTVCSIGKINYVHQEYDEVNNEFIINDNEIIDNDNVIDFENTDHSLISEQEIEDSLVNIDELNDQNTKNENND" }, "paralog": false, "identity": 0.919732441471572, "score": 1090.0, "evalue": 0.0, "description": "Internal stop codon at 157058, 157193. Nonsense mutation around 157058, 157193.", "psc": { "uniref90_id": "UniRef90_Q9PR25", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_Q9PR25", "cog_id": "COG0653", "cog_category": "U", "go_ids": [ "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0006886", "GO:0008564", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR25", "EC:7.4.2.8", "UniRef:UniRef50_Q9PR25", "COG:COG0653", "COG:U", "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0006886", "GO:0008564", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR25" ], "product": "Protein translocase subunit SecA" } }, "id": "GPKLONFDEH_280", "locus": "GCA000006625_00245" }, { "type": "cds", "sequence": "contig_1", "start": 157411, "stop": 158424, "strand": "+", "frame": 1, "gene": null, "product": "Excinuclease ABC subunit B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PB45" ], "nt": "ATGGATGATTTTAAAAAATTTGAATTAATAAGCGATTATAAACCAGCTGGTGACCAACAAAAGGCAATTGATGAAATGGTTAATAATATTAATCAAGGTATTCAACGACAAGTATTATTAGGGGCAACAGGAACAGGAAAAACATTTAGTGTGGCTAATGTAATTGCAAAAACCCAATTAAAGACTTTAGTTCTTGCTCATAATAAAACATTAGCTGCACAATTATTTGCTGAACTTAAAGAAATGTTTCCTCATAATAAAGTTGAATATTTTGTTTCATATTTTGATTATTATCAACCAGAAGCTTATAAACCTATAACTGATACTTATATTGAAAAAGATTCAGTAACAAATGCAGAAATTGAGATGATGCGTTTATCAACTATTAATTCATTGGCTACAAGAAATGATGTCATTGTTGTTGCTTCTGTTGCATGTATTTATGCATCTGTTTCTCCTATAGAATTTACTAAAAAGAATTTATATTTACATGTTGGTGAATTAATCGAATTTGAACAAATTAAATATAAATTAGTTCAATTAGGTTATGAACGAAAAGATTATGATTTAGTGCCAGGAACATTTAGGATTCGTGGTGATTTAATTGAAATTATGTCTGGATATAGTGATAAATTTAAAATAAGAATATCAATGTTTGGAAATGAAGTTGAATCAATTGATCTTTGCGATCCAATTACTAATAATATTATTTCTAAAGAACAAAGGTTTATTTTACGAAGTGCAAGTGAATATATTTTTGATGATTCGCGTTTAGCAATAGCGATTGAAAATATTAAAAATGAACTAGATGAACGAGTTAAATTTTTTCTAAAAAACAATCAATTAATTGAGGCGCAACGGATTGAGCAACGAACTAAACAAGATCTTGAAAGTATTGTAGAGTTTGGTTTTTGTAGTGGAGTTGAAAATTATTATCGTCATCTTGAACATCGTGAACCTTTTCAAACACCATGGACAATTTTTGATTTTTTTTCATATAACAATCAAGATTGA", "aa": "MDDFKKFELISDYKPAGDQQKAIDEMVNNINQGIQRQVLLGATGTGKTFSVANVIAKTQLKTLVLAHNKTLAAQLFAELKEMFPHNKVEYFVSYFDYYQPEAYKPITDTYIEKDSVTNAEIEMMRLSTINSLATRNDVIVVASVACIYASVSPIEFTKKNLYLHVGELIEFEQIKYKLVQLGYERKDYDLVPGTFRIRGDLIEIMSGYSDKFKIRISMFGNEVESIDLCDPITNNIISKEQRFILRSASEYIFDDSRLAIAIENIKNELDERVKFFLKNNQLIEAQRIEQRTKQDLESIVEFGFCSGVENYYRHLEHREPFQTPWTIFDFFSYNNQD", "aa_hexdigest": "154fad3cf5a061451ea69eb5fa820738", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00056E9490", "query_cov": 0.9792284866468842, "subject_cov": 0.9348441926345609, "identity": 0.742, "score": 488.0, "evalue": 3.77e-171, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_A0A3B0PB45", "kegg_orthology_id": "K03702" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PB45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PB45" ], "product": "Excinuclease ABC subunit B" }, "genes": [], "id": "GPKLONFDEH_281", "locus": "GCA000006625_00246" }, { "type": "cds", "sequence": "contig_1", "start": 158473, "stop": 159411, "strand": "+", "frame": 1, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "EC:3.1.25.-", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016787", "GO:0016887", "KEGG:K03702", "SO:0001217", "UniRef:UniRef50_P47319", "UniRef:UniRef90_Q9PR24" ], "nt": "ATGCATAATACAGACCGTAGTCGTAAACAAACATTAGTTGAATACGGATTTCGTTTACCTAGCGCTTTAGATAATCGGCCTTTAAATTATGATGAATTTAATAAAAAACTTTCCAAAACGATTTATGTTTCTGCCACACCAAACGATGAAGAAATTGCGTTATCAGATAATCATATAGTTAGTCAAATTGTTCGTCCTACAGGATTATTAGATCCAATTATTGAAATTAGAAAAACAGAACACCAAATTGATGATTTAATTAATGAATTGATGCTTTTAAAAAACAAGAATCAACGAGCATTTATTACTGTTATGACAATTAGAATGGCTGAGGATTTAACAAATTATTTAAATAATACAAAAATTAAAGCTGCTTATTTACATAATGAATTAAAAACACTAGAGCGTAGTGTTATTATCAATAAATTAAGAAAAGGAATTTATGATTGTGTTGTTGGAATTAATCTTTTAAGAGAAGGATTAGATGTTCCAGAAGTTGCAGGTGTTTTTATTTTTGATGCTGATAAACCTGGTTTTTTTAGAAGTGATAAATCTTTAATTCAAATTATTGGACGTGCAGCACGTAATGCTGATGGTAAAGTAATTATGTATGCTGATGTAATAACACAAGCCATGCAAACCGCAATCAATGAAACAAAACGACGACGTGAAATTCAATTAGCTTTTAATTTAAAACATAATATTATCCCTAAAACAATTATTAAACCAATTCACGAAGATTTAAGTGGCCATGATTATAAACAAAATGCGGAATTATATGCTGCAAAAGCTTCAAAAAACGAGTACAATCAAAAAATCAAAGAATTAAAGAAAAAAATGGAAGAAGCTGCAAAAAAACGTGAATATGAAGTTGCTGCTCAATATCGCGATATGATTGTTGAATTAGAAGCCATTAAGCAAAGTGTAAAAAGCAAATAA", "aa": "MHNTDRSRKQTLVEYGFRLPSALDNRPLNYDEFNKKLSKTIYVSATPNDEEIALSDNHIVSQIVRPTGLLDPIIEIRKTEHQIDDLINELMLLKNKNQRAFITVMTIRMAEDLTNYLNNTKIKAAYLHNELKTLERSVIINKLRKGIYDCVVGINLLREGLDVPEVAGVFIFDADKPGFFRSDKSLIQIIGRAARNADGKVIMYADVITQAMQTAINETKRRREIQLAFNLKHNIIPKTIIKPIHEDLSGHDYKQNAELYAAKASKNEYNQKIKELKKKMEEAAKKREYEVAAQYRDMIVELEAIKQSVKSK", "aa_hexdigest": "292305a417d2c364a71f362365bee818", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002024C376", "query_cov": 0.9871794871794872, "subject_cov": 0.9871794871794872, "identity": 0.5710000000000001, "score": 332.0, "evalue": 1.2e-110, "valid": false, "product": "helicase-related protein", "uniref50_id": "UniRef50_A0A519SUY2", "kegg_orthology_id": "K03702" }, "genes": [ "uvrB" ], "truncated": "5-prime", "pseudogene": { "start": 158173, "stop": 159411, "observations": { "mutation": [ 158422 ], "stop": [ 158422 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR24", "query_cov": 1.0, "subject_cov": 0.46846846846846846, "identity": 1.0, "score": 600.0, "evalue": 6.2e-211, "gene_start": 1, "gene_end": 312, "reference_start": 355, "reference_end": 666, "reference_sequence": "MDDFKKFELISDYKPAGDQQKAIDEMVNNINQGIQRQVLLGATGTGKTFSVANVIAKTQLKTLVLAHNKTLAAQLFAELKEMFPHNKVEYFVSYFDYYQPEAYKPITDTYIEKDSVTNAEIEMMRLSTINSLATRNDVIVVASVACIYASVSPIEFTKKNLYLHVGELIEFEQIKYKLVQLGYERKDYDLVPGTFRIRGDLIEIMSGYSDKFKIRISMFGNEVESIDLCDPITNNIISKEQRFILRSASEYIFDDSRLAIAIENIKNELDERVKFFLKNNQLIEAQRIEQRTKQDLESIVEFGFCSGVENYYRHLEHREPFQTPWTIFDFFSYNNQDWLLIVDESHISLPQVKGMHNTDRSRKQTLVEYGFRLPSALDNRPLNYDEFNKKLSKTIYVSATPNDEEIALSDNHIVSQIVRPTGLLDPIIEIRKTEHQIDDLINELMLLKNKNQRAFITVMTIRMAEDLTNYLNNTKIKAAYLHNELKTLERSVIINKLRKGIYDCVVGINLLREGLDVPEVAGVFIFDADKPGFFRSDKSLIQIIGRAARNADGKVIMYADVITQAMQTAINETKRRREIQLAFNLKHNIIPKTIIKPIHEDLSGHDYKQNAELYAAKASKNEYNQKIKELKKKMEEAAKKREYEVAAQYRDMIVELEAIKQSVKSK" }, "paralog": false, "identity": 0.9975728155339806, "score": 808.0, "evalue": 2.96e-295, "description": "Internal stop codon at 158422. Nonsense mutation around 158422.", "psc": { "uniref90_id": "UniRef90_Q9PR24", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "ec_ids": [ "3.1.25.-" ], "uniref50_id": "UniRef50_P47319", "cog_id": "COG0556", "cog_category": "L", "kegg_orthology_id": "K03702", "go_ids": [ "GO:0003677", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016787", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR24", "EC:3.1.25.-", "UniRef:UniRef50_P47319", "COG:COG0556", "COG:L", "KEGG:K03702", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016787", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47319", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47319" ], "product": "UvrABC system protein B" } }, "id": "GPKLONFDEH_282", "locus": "GCA000006625_00247" }, { "type": "cds", "sequence": "contig_1", "start": 159582, "stop": 160409, "strand": "+", "frame": 3, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872", "SO:0001217", "UniRef:UniRef50_Q9PR23", "UniRef:UniRef90_Q9PR23" ], "nt": "GTGCCAGACCATGTTTATGATCAAACAATGCGGGAGTTAATCGAACTTGAAAATAATTATCCTCAATTTAAAACTAACAATTCACCAAGTGTAAAGGTTGGCGGCTTTGTTTCAGAAAAATTTAATAAAGTAAAACATAAAAGACCAATGTTATCACTTTCAAATGCTTTTAATGATGATGATTTAAAAAAATTTGATCAAGATAATCAAAATGCTAGTGTCGATTTAAAAGGGTACGTAGTTGAACCTAAAATTGATGGTTTAAGCATTTCTATTATTTATAAAAATGCCAAATTACACCAGGCAATTACAAGAGGAGATGGGATTAATGGCGAAGATGTTACTTCCAATATTTTAACGATTAAAGACATTCCTCACTATATTGATCAAAAATATAAAGACTACGAAATTGAAGTTCGTGGTGAAGTCTATATGGCTTTTCATGATTTTTATGAAATGAATGATAATTTAGAAGAAAGTGATAAAAAATTTGCTAATCCAAGAAATGCTGCTGCTGGAACATTAAGAAGTCTAGATAATTCAATTGTTGCAGAACGTAAATTAAGTGCTTTTATGTATTATTTGGTTAATGCACAAGAACTAGGAATTAAAACGCATTATGAAAGTATTCAATTTTTAAGAGATAATAAATTTAAAGTTAGTGATTTAATTGTTAAAGTCGATACAATTAATGAAGTTATTAATCAAATTGATTGTTATACTAAAGTGCGTGATAAACTTTCATATATGATTGATGGTATTGTTATTAAAATTAATAATTTAGAAGTTTATGATGAGATTGGTTATACTTCTAAATTTCCTAAATGA", "aa": "MPDHVYDQTMRELIELENNYPQFKTNNSPSVKVGGFVSEKFNKVKHKRPMLSLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISIIYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQKYKDYEIEVRGEVYMAFHDFYEMNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQFLRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSKFPK", "aa_hexdigest": "7a451a08b128203c34586d0e8f1bc665", "start_type": "GTG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 159495, "stop": 160712, "observations": { "mutation": [ 159546, 160407 ], "stop": [ 159546, 160407 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR23", "query_cov": 1.0, "subject_cov": 0.4086181277860327, "identity": 0.996, "score": 544.0, "evalue": 1.7e-189, "gene_start": 1, "gene_end": 275, "reference_start": 30, "reference_end": 304, "reference_sequence": "MDKIKAKIDELKQKLDQWNYEYYVLDDPSVPDHVYDQTMRELIELENNYPQFKTNNSPSVKVGGFVSEKFNKVKHKRPMLSLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISIIYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQKYKDYEIEVRGEVYMAFHDFYEMNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQFLRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSKFPKWAIAYKFPANVVSSQLLEIINDVGRTGKISYVAKIKPILLDGSMVEYATLHNFDFIKEKDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEPIINCPSCQSVLVRENDEVDQRCLNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFILTSDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANFDELTNVDACGEKAALSLINWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKNKTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDYVLVGTEAGTKLEKAKLLGVKIIENEFWKKDNNF" }, "paralog": false, "identity": 0.9654320987654321, "score": 775.0, "evalue": 5.21e-283, "description": "Internal stop codon at 159546, 160407. Nonsense mutation around 159546, 160407.", "psc": { "uniref90_id": "UniRef90_Q9PR23", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_Q9PR23", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR23", "EC:6.5.1.2", "UniRef:UniRef50_Q9PR23", "COG:COG0272", "COG:L", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR23" ], "product": "DNA ligase" } }, "id": "GPKLONFDEH_283", "locus": "GCA000006625_00248" }, { "type": "cds", "sequence": "contig_1", "start": 160536, "stop": 161495, "strand": "+", "frame": 3, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872", "SO:0001217", "UniRef:UniRef50_Q9PR23", "UniRef:UniRef90_Q9PR23" ], "nt": "ATGGTTGAATACGCTACTTTACATAACTTTGATTTTATTAAAGAAAAAGACATTCGAATTAATGATGAAATTAAAATTTATAAAGCAGGGGATGTAATTCCTTATGTTGATGGTGTTGATTTATCAAAACGTTTAGCAAATAGTGTACCTTATGAACCAATTATAAATTGCCCTTCATGCCAATCAGTTTTAGTACGTGAAAATGATGAAGTTGACCAACGCTGCTTAAATATTTATGGATGTAAAAAAATCAATATTGAAAAAATCGTTTATTTTGTTTCACGTAATTGCATGAATATTGAGGGAATGAGTGATGCCATTATTAATAAATTTTATGATGCTAATTTAATTAAAAACATTGCAGATTTATACTATTTGCAAAAACACAAAGAATTTATTTTAACTAGTGATTTTAAAATTAAAGAAAAAAGTTTTAGTAATTTAATTAATAGTATTAATAATTCAAAAAAATGTTCTTTAGAATTTTTATTAACAGCTTTTGGAATTCGTCATGTTGGACCAAATTTAGCTAAAAAAATAGCTAAACAATTTAAAACAATGACTGCTTTAATGCACGCTAATTTTGATGAATTAACTAATGTTGATGCATGTGGGGAAAAAGCAGCCTTATCATTAATTAATTGGTTTAATGATGAGCATAATGTTTCTTTAGTTAATCAATTACAACAAGTTGGTGTAAATATGGAATATATTGATGATTTTATTTATGATGATAACATCAATATAATTGATGAATATAAAAATAAAACTTTTGTTATTACTGGTTCATTTAGTATTAGCCGTGATGAAATTAAAACTATTTTAGAGAAATATTATCATGCAAAAGTTAAAAACAGTGTTTCTAAAAAAACAGATTATGTTTTAGTTGGCACTGAAGCGGGTACGAAATTAGAAAAAGCTAAATTATTAGGTGTGAAAATTATTGAAAATGAATTTTGA", "aa": "MVEYATLHNFDFIKEKDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEPIINCPSCQSVLVRENDEVDQRCLNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFILTSDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANFDELTNVDACGEKAALSLINWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKNKTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDYVLVGTEAGTKLEKAKLLGVKIIENEF", "aa_hexdigest": "795b5edd976fd614ae58000e05993f80", "start_type": "ATG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 160236, "stop": 161516, "observations": { "mutation": [ 160407, 161493 ], "stop": [ 160407, 161493 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR23", "query_cov": 1.0, "subject_cov": 0.4739970282317979, "identity": 1.0, "score": 635.0, "evalue": 1.13e-224, "gene_start": 1, "gene_end": 319, "reference_start": 348, "reference_end": 666, "reference_sequence": "MDKIKAKIDELKQKLDQWNYEYYVLDDPSVPDHVYDQTMRELIELENNYPQFKTNNSPSVKVGGFVSEKFNKVKHKRPMLSLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISIIYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQKYKDYEIEVRGEVYMAFHDFYEMNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQFLRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSKFPKWAIAYKFPANVVSSQLLEIINDVGRTGKISYVAKIKPILLDGSMVEYATLHNFDFIKEKDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEPIINCPSCQSVLVRENDEVDQRCLNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFILTSDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANFDELTNVDACGEKAALSLINWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKNKTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDYVLVGTEAGTKLEKAKLLGVKIIENEFWKKDNNF" }, "paralog": false, "identity": 0.9953051643192489, "score": 839.0, "evalue": 1.71e-307, "description": "Internal stop codon at 160407, 161493. Nonsense mutation around 160407, 161493.", "psc": { "uniref90_id": "UniRef90_Q9PR23", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_Q9PR23", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR23", "EC:6.5.1.2", "UniRef:UniRef50_Q9PR23", "COG:COG0272", "COG:L", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR23" ], "product": "DNA ligase" } }, "id": "GPKLONFDEH_284", "locus": "GCA000006625_00249" }, { "type": "cds", "sequence": "contig_1", "start": 161753, "stop": 162154, "strand": "+", "frame": 2, "gene": null, "product": "MPN527 family putative ECF transporter permease subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6S5", "UniRef:UniRef90_A0AAC9X6S5" ], "nt": "TTGAAGATAAATATAACAAAAGAAAAAACAATTTTTAAAAAAAATTATAAAAAAAAACATAGTATATATTATTCAACATCTAAAATTATTAAAGACTACACACTAAGTGCAGTAATGTTAGGTTTAGGAATTTTGGTTGCATATTTAAGTCACTTTATTCGTTTTAATTTCTTAGCTTTTGATTTTTCTTTGTTTACACTTGTTTATTTAATTTATAAAGTTAAATATCGCTTTGTATATTTAGTTACAATTTTATTAAGTTTAGCTAATTTATTGCACGGAAGTCCTTCATGGATCGGAACAATGGTTTTAAGTTTAAATAACTTATTTTTTGTTACTATGGTTATTCTTTTTAAAAAGTTATTTATTCCAAAAAGACCAAAATTTTATCTTAATTTATGA", "aa": "MKINITKEKTIFKKNYKKKHSIYYSTSKIIKDYTLSAVMLGLGILVAYLSHFIRFNFLAFDFSLFTLVYLIYKVKYRFVYLVTILLSLANLLHGSPSWIGTMVLSLNNLFFVTMVILFKKLFIPKRPKFYLNL", "aa_hexdigest": "f531acf65c3e813ae8585876f3e459f6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 161753, "stop": 162448, "observations": { "mutation": [ 162152, 162230, 162329 ], "stop": [ 162152, 162230, 162329 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6S5", "query_cov": 1.0, "subject_cov": 0.5757575757575758, "identity": 0.97, "score": 239.0, "evalue": 2.26e-78, "gene_start": 1, "gene_end": 133, "reference_start": 1, "reference_end": 133, "reference_sequence": "MKINITKGKTIFKRNYKKKHSIYYSTSKIIKDYTLSAVMLGLGILIAYLSHFIRFNFLAFDFSLFTLVYLIYKVKYRFVYLVTILLSLANLLHGSPSWIGTMVLSLNNLFFVSMVILFKKLFIPKRPKFYLNLWVLVLSSFLTTVFNVIMNGILYTPLYWYTFKITPTLNFLEVQKIYEKHPTLFLLNIKSYWLGIIALYTSFNLIKNIIISFISLTILKIFADKKTIITY" }, "paralog": false, "identity": 0.9653679653679653, "score": 434.0, "evalue": 2.06e-157, "description": "Internal stop codon at 162152, 162230, 162329. Nonsense mutation around 162152, 162230, 162329.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6S5", "product": "MPN527 family putative ECF transporter permease subunit", "uniref50_id": "UniRef50_A0AAC9X6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6S5", "UniRef:UniRef50_A0AAC9X6S5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6S5" ], "product": "ECF transporter S component" } }, "id": "GPKLONFDEH_285", "locus": "GCA000006625_00250" }, { "type": "cds", "sequence": "contig_1", "start": 162462, "stop": 162995, "strand": "+", "frame": 3, "gene": "cysS", "product": "cysteine--tRNA ligase", "db_xrefs": [ "COG:COG0215", "COG:J", "EC:6.1.1.16", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270", "KEGG:K01883", "SO:0001217", "UniRef:UniRef50_Q9PR21", "UniRef:UniRef90_Q9PR21" ], "nt": "ATGAAATTATATGATTCTTATTCTAATCAATTAGTAGAAATTAATGATGAATTAATTTCAATTTATAATTGTGGACCAACCGTTTATAATCATATCCATATTGGCAATGCTCGGCCTTTAATTACTATGGACGTGTTATATCGTTTTTTAAAAAAACATAATATCAAAACTAAATATGTTTTAAATATTACTGATATTGATGACAAAATCATTAATTATGCTCTAGCTAATAACCTAAAAGAATTAGAGGTTAGTGAATATTATTTTAATGAATATTTAAAAATTAAAAAAGCTTTAAATACACTAGAAATGATTAATCCTAAAGTATCAACACATATGGATAAAATTATTGATTATATCCAAAAATTGATTGATAAACAAGCAGGTTATTTTATTGGTGATGATGTTTATTTTGATACAAAAAAAGCTTTAAATTACGGACAATTATCGAAACGTGATTTAGAAAATGATATTGTTGGTATGCGTATTGAAAGCGCAGCTAATAAACACAATCCTAATGATTTTATTTTATGA", "aa": "MKLYDSYSNQLVEINDELISIYNCGPTVYNHIHIGNARPLITMDVLYRFLKKHNIKTKYVLNITDIDDKIINYALANNLKELEVSEYYFNEYLKIKKALNTLEMINPKVSTHMDKIIDYIQKLIDKQAGYFIGDDVYFDTKKALNYGQLSKRDLENDIVGMRIESAANKHNPNDFIL", "aa_hexdigest": "e792f8caccff44a245a3d12612c470ad", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "cysS" ], "truncated": "3-prime", "pseudogene": { "start": 162462, "stop": 163298, "observations": { "mutation": [ 162993, 163020, 163032, 163200 ], "stop": [ 162993, 163020, 163032, 163200 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR21", "query_cov": 1.0, "subject_cov": 0.4059633027522936, "identity": 1.0, "score": 359.0, "evalue": 6.19e-122, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKLYDSYSNQLVEINDELISIYNCGPTVYNHIHIGNARPLITMDVLYRFLKKHNIKTKYVLNITDIDDKIINYALANNLKELEVSEYYFNEYLKIKKALNTLEMINPKVSTHMDKIIDYIQKLIDKQAGYFIGDDVYFDTKKALNYGQLSKRDLENDIVGMRIESAANKHNPNDFILWKKTNKGIMWNTPWGIGRPGWHSECSCLINTYIGEQVSIHGGGIDLKFPHHENENAQNQVLYNKNLAKVWMHFGLVNINNEKMSKSLNNFILVKDLLAEYDYQVVRWFFYQADYKQPIKFSHEIMKQNEKEILKIKNAIYNAKNYLYFNNQLKSLTQIDHFELFDERINDDLDFVGIVDLIHISVKKINILIKENKDMNELKLNLTQLLYMLDILGINFVDLHNDENLALLNTWKNYVDKKDYVKADELRKQLINIGIL" }, "paralog": false, "identity": 0.9856115107913669, "score": 555.0, "evalue": 2.16e-201, "description": "Internal stop codon at 162993, 163020, 163032, 163200. Nonsense mutation around 162993, 163020, 163032, 163200.", "psc": { "uniref90_id": "UniRef90_Q9PR21", "gene": "cysS", "product": "cysteine--tRNA ligase", "ec_ids": [ "6.1.1.16" ], "uniref50_id": "UniRef50_Q9PR21", "cog_id": "COG0215", "cog_category": "J", "kegg_orthology_id": "K01883", "go_ids": [ "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR21", "EC:6.1.1.16", "UniRef:UniRef50_Q9PR21", "COG:COG0215", "COG:J", "KEGG:K01883", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR21" ], "product": "Cysteine--tRNA ligase" } }, "id": "GPKLONFDEH_286", "locus": "GCA000006625_00251" }, { "type": "cds", "sequence": "contig_1", "start": 163074, "stop": 163202, "strand": "+", "frame": 3, "gene": null, "product": "tRNA synthetases class I catalytic domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBA8" ], "nt": "TTGATCAACACTTATATTGGTGAACAAGTTAGTATTCATGGTGGTGGAATTGATTTAAAATTTCCCCACCATGAAAATGAAAATGCTCAAAATCAAGTTTTATATAATAAAAATTTAGCAAAAGTATGA", "aa": "MINTYIGEQVSIHGGGIDLKFPHHENENAQNQVLYNKNLAKV", "aa_hexdigest": "12f789fc56cb30128467d6e356cbfb35", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBA8", "query_cov": 0.8333333333333334, "subject_cov": 0.8333333333333334, "identity": 0.5429999999999999, "score": 44.3, "evalue": 4.62e-05, "valid": false, "product": "tRNA synthetases class I catalytic domain-containing protein", "uniref50_id": "UniRef50_A0A432FBA8" }, "pscc": { "uniref50_id": "UniRef50_A0A432FBA8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBA8" ], "product": "tRNA synthetases class I catalytic domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_287", "locus": "GCA000006625_00252" }, { "type": "cds", "sequence": "contig_1", "start": 163203, "stop": 163694, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01406.26" ], "nt": "ATGCATTTTGGATTAGTTAATATTAATAATGAGAAAATGTCAAAATCTTTAAATAATTTTATTTTAGTAAAAGATTTATTAGCTGAATACGATTATCAAGTTGTGCGTTGGTTTTTTTATCAAGCAGATTATAAGCAACCAATTAAATTTTCGCATGAAATCATGAAGCAGAACGAAAAAGAAATTCTTAAAATTAAAAATGCTATTTATAACGCAAAAAACTATTTATACTTCAATAATCAACTAAAATCATTAACACAAATTGATCATTTTGAGCTTTTTGATGAACGCATTAATGATGATTTAGATTTTGTAGGTATTGTTGATCTAATTCATATTAGTGTTAAAAAAATAAATATTTTAATTAAAGAAAATAAGGATATGAATGAATTAAAACTGAATTTAACTCAATTATTATATATGTTAGATATATTGGGTATTAATTTTGTTGATTTGCATAATGATGAGAATTTAGCATTATTAAATACTTGA", "aa": "MHFGLVNINNEKMSKSLNNFILVKDLLAEYDYQVVRWFFYQADYKQPIKFSHEIMKQNEKEILKIKNAIYNAKNYLYFNNQLKSLTQIDHFELFDERINDDLDFVGIVDLIHISVKKINILIKENKDMNELKLNLTQLLYMLDILGINFVDLHNDENLALLNT", "aa_hexdigest": "b7afa85f1fa6bb8fde76edd219527084", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 58, "aa_cov": 0.3558282208588957, "hmm_cov": 1.0, "evalue": 4.095589541729107e-18, "score": 61.326812744140625, "start": 1, "stop": 58 } ], "seq_stats": { "molecular_weight": 19447.252500000013, "isoelectric_point": 5.499934196472167 }, "id": "GPKLONFDEH_288", "locus": "GCA000006625_00253" }, { "type": "cds", "sequence": "contig_1", "start": 163774, "stop": 163923, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08032.17" ], "nt": "ATGAATTATAATTTTGGCAAAAAAGCATTATTAGATGCAATTGGTAACAAACAATCAATTTCAAAAGTGTATTTATTATCAAAAAATTATGAGTTAATTAATTTAATTAATAAAAATAAAATTACTTATGAAATTGTTAATGAAGCATGA", "aa": "MNYNFGKKALLDAIGNKQSISKVYLLSKNYELINLINKNKITYEIVNEA", "aa_hexdigest": "257d76286064b4c4b47bc63c5661ba08", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08032.17", "name": "SpoU_sub_bind", "length": 51, "aa_cov": 0.9387755102040817, "hmm_cov": 1.0, "evalue": 1.3746415344160197e-07, "score": 27.41458511352539, "start": 4, "stop": 49 } ], "seq_stats": { "molecular_weight": 5635.488699999999, "isoelectric_point": 9.347996330261232 }, "id": "GPKLONFDEH_289", "locus": "GCA000006625_00254" }, { "type": "cds", "sequence": "contig_1", "start": 164119, "stop": 164268, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATGCTATTATTTATAAAAAGCATCACCAAGCAAGCATTAATGATTTAGTAATTAAAACAAGTATGGGTGCAATTAATTATATTTATATGATCGAAGTCAATAATTTATCAAATACAATAAAAAACCTTCAAGATGAAGGTTTTTGA", "aa": "MDAIIYKKHHQASINDLVIKTSMGAINYIYMIEVNNLSNTIKNLQDEGF", "aa_hexdigest": "cb5080d8780f592098625e3bf642a6c1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5613.4003, "isoelectric_point": 5.985168647766113 }, "id": "GPKLONFDEH_290", "locus": "GCA000006625_00255" }, { "type": "cds", "sequence": "contig_1", "start": 164362, "stop": 164493, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTCCATTAATTTTAAAAAATGCAGATCAAAGCATACGAATTGATATGTTTGGTGATGTACAATCTTTAAATGTTGGTGTAGCAACAGGAATTATCTTAGCGCATTATCGCACTAAAATAAAACCCTAG", "aa": "MSPLILKNADQSIRIDMFGDVQSLNVGVATGIILAHYRTKIKP", "aa_hexdigest": "322cdd67d3bb400dd1f7324630959348", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4725.5344000000005, "isoelectric_point": 9.520965003967284 }, "id": "GPKLONFDEH_291", "locus": "GCA000006625_00256" }, { "type": "cds", "sequence": "contig_1", "start": 164793, "stop": 165686, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AC84", "UniRef:UniRef90_A0AAC9X6P0" ], "nt": "ATGAAAAAAAATAAATATAAACTTCTTATTAAATCACTAGTTATTACATCTTTTTTAGTTACTACTTCAAGTGTTATTGTAGCTTGTACTAATAAAGAAAAAGAGCAACATTTAACTGAATTAAAAAAATTATCTCAACATGCAAAACAAATATATAATGAAATTAAAACTAATGAATTATTTAAAAAGGAGGCTTCTGTTTTAAATGATCAAATTAATAAAGTTTTAAAAATTAATGAACAAAATAATTTAGATGTCATTACTAAAAATTATAATGATTTAAAAAAATTATTAGATGAAATTCAAGAGAAAATTTCTAAAGAAAAAGAAAAAGAAAAGCAACAAAACGCTTATCTTAATAAAGAAATTAACATTAAATTAGAAAATTTAAATCAAATAGTTAATCATAATTTATTAAAAGGAAATAAAGAATCATTTTTTAATCAAATTATTAATTTCACTCCAATTACAACAGAAATTCAAACTATTATCAATCAAAATCAAAATCTTTTAGATTTCAAAAATGAAGAACTTGAATTAAAATTAAAAAATTTAGACCTTTTCATTAAAGAATTAGATCATAAATTTAAAAATATAATAGATCAACTATTTAATATTGAAGATTCATACATTAATAAGTTTCTAAAATTTATTAATAACCAGGACTTTATTAAGGATCAAAAATATCAAGAAAGAGAAAAGTTTAATCCGCAAGAATATTTAGAATTAACAAAATCTATTCAAAGTAGTTTTAATAAATTAAAAAACGTTACTATCAATTCAAACCTAGACCAATACCTAAATTCACTTAATTTAGTGTTATTATCTTTAACTAATGAAACTAAAGCCTTTTTTACTAAATTTGAAGATCAAATTAAAAAATATTTTAAGTGA", "aa": "MKKNKYKLLIKSLVITSFLVTTSSVIVACTNKEKEQHLTELKKLSQHAKQIYNEIKTNELFKKEASVLNDQINKVLKINEQNNLDVITKNYNDLKKLLDEIQEKISKEKEKEKQQNAYLNKEINIKLENLNQIVNHNLLKGNKESFFNQIINFTPITTEIQTIINQNQNLLDFKNEELELKLKNLDLFIKELDHKFKNIIDQLFNIEDSYINKFLKFINNQDFIKDQKYQEREKFNPQEYLELTKSIQSSFNKLKNVTINSNLDQYLNSLNLVLLSLTNETKAFFTKFEDQIKKYFK", "aa_hexdigest": "5008dff98922e96c5757ef7e92a5a537", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 164502, "stop": 165686, "observations": { "mutation": [ 164564, 164597, 164795 ], "stop": [ 164564, 164597, 164795 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6P0", "query_cov": 1.0067340067340067, "subject_cov": 0.7550505050505051, "identity": 0.9570000000000001, "score": 452.0, "evalue": 5e-157, "gene_start": 1, "gene_end": 297, "reference_start": 1, "reference_end": 299, "reference_sequence": "MKKNKYKLLIKSLVITSFLVTTSSVIVACTNKEKEQHLTKLKKLSQHAKQIYNEIKTNELFKNEASVLNDQINKVLKINEQNNLDVITKNYNDLKKLLDEIQEKISKEKEKEKQQNAYLNKEINIKLENLNQIVNHNLLKENKESFFNQIINFTPITTEIQTIINQNQNQNLLDFKNEELELKLKNLDLFIKELDHKFKNIIDQLFNIEDSYINKFLKFINNQDFIKDQKYKEREKFNPQEYLELTKSIESSFNKLKNVTINSNLDQYLNSLNLVLLSLTNETKTFFTKFEDQIKKYFKWIAYGKDDTLTKTFKRIVQEQKRNEFIEQTEKALLTFPTEINQKFKMFVDFYTLKNDKDETEYQKIWKSKRNEVDKVWFGIAECFGSGKCTDKSKPS" }, "paralog": false, "identity": 0.9267676767676768, "score": 643.0, "evalue": 7.54e-235, "description": "Internal stop codon at 164564, 164597, 164795. Nonsense mutation around 164564, 164597, 164795.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6P0", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAP9AC84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6P0", "UniRef:UniRef50_A0AAP9AC84" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AC84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AC84" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_292", "locus": "GCA000006625_00257" }, { "type": "cds", "sequence": "contig_1", "start": 165698, "stop": 166108, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAACTTTCCCTAGAACAGAGACAATTAATGAATTTAATGCTGGCAAACTTCCACGCTATCGTGAATATGAAATTAATAAATATGATAAAATCCAAGAAAATATTATTACTACTAATAATTACTTATCAACAGTAGGGACAACTAAAATTAGTAATCAATATTTTGATGTTGGTTCTGGTTCATCAGGATCAGTAGTTTATGATGAAAATGGTGATTTAGTTGGAATGATTTCTTTTGGCGCCCATGAAAATGCAAAATTTACAGCCGGAGGTATGATTGTTTTTGATTCTCTAATTTCTGGATTTTTTGGAGATAAAAACAATTTCGCTAATCGTACATCTTTAATTCAACAAATCAAACAATTAGCCTATTTATATCCAGAAAAATATGAAGATATTTATAAATAG", "aa": "MSTFPRTETINEFNAGKLPRYREYEINKYDKIQENIITTNNYLSTVGTTKISNQYFDVGSGSSGSVVYDENGDLVGMISFGAHENAKFTAGGMIVFDSLISGFFGDKNNFANRTSLIQQIKQLAYLYPEKYEDIYK", "aa_hexdigest": "94f990b6094b86d57dde40f0f5d4328f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15376.945599999992, "isoelectric_point": 5.125763130187988 }, "id": "GPKLONFDEH_293", "locus": "GCA000006625_00258" }, { "type": "cds", "sequence": "contig_1", "start": 166703, "stop": 166831, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATGATAAATCTAATAATCAATATACAACAAGAATTTATAAAAACAATGAACAGAATTTTTTACCAATTAAAAATGCTCGTGATCGTGTAATTTCATTAAGTTTAATTCAAGATGGTACATGA", "aa": "MEDDKSNNQYTTRIYKNNEQNFLPIKNARDRVISLSLIQDGT", "aa_hexdigest": "dbc250d66352f5e2a666a5e574fb86e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4944.4085000000005, "isoelectric_point": 6.026888465881346 }, "id": "GPKLONFDEH_294", "locus": "GCA000006625_00259" }, { "type": "cds", "sequence": "contig_1", "start": 166919, "stop": 167149, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGGTCTCAAATTTCATTAGCAGTCCCTTATAAAACTTTAAAAAATAAAAAAGTTATTGAAGATGCTGAATTTGTTTTAAATTATGGTATTGGAAATCAAGAGTACGAATATGAACTCTACAAAAAAATTAAGCAAGATTTTCGTTTTCAAATATCTTATATAAATGATGAATTAAAAATTAAAATTATTTCTAAAAATAACATTAGTGTTTTTGATAATATTTGA", "aa": "MLRSQISLAVPYKTLKNKKVIEDAEFVLNYGIGNQEYEYELYKKIKQDFRFQISYINDELKIKIISKNNISVFDNI", "aa_hexdigest": "b8a7ee8f46a3e8287ab1668afe48f925", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9067.375899999992, "isoelectric_point": 8.830121803283692 }, "id": "GPKLONFDEH_295", "locus": "GCA000006625_00260" }, { "type": "cds", "sequence": "contig_1", "start": 167159, "stop": 167476, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGAAATTTTATTTCATTAGTTCAATATGAAAATACCTACTTATTTTATCAAAATAAAACAAACGAAGGTTTTTATGATTTCATAACTAAAAAAGCTGATTTTATACTTGTAAATAATAATGAAATCAAAAATTTACATAAAACTACATTAAAAGATAATCCAGATTTGATCTTTAAAAAAGATAAAGATAATTATTTTCAATTAACAAAAAAAATAAATAAGATTAAAAATTTAAATCTTGTAAATGAAAAAAATTTAGTTTTTAATATTACTTTTGGTTTAGATCCAAATCTCATCAAAGAAACTAACCTGTGA", "aa": "MRNFISLVQYENTYLFYQNKTNEGFYDFITKKADFILVNNNEIKNLHKTTLKDNPDLIFKKDKDNYFQLTKKINKIKNLNLVNEKNLVFNITFGLDPNLIKETNL", "aa_hexdigest": "7ec4f7f6e31ae5363fc84e751af4164b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12556.27779999998, "isoelectric_point": 9.24742565155029 }, "id": "GPKLONFDEH_296", "locus": "GCA000006625_00261" }, { "type": "cds", "sequence": "contig_1", "start": 167771, "stop": 167965, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAAAATGAAGTATATGGATCAGCATTTTTTGATTTTAATGAACAAACTTTAAAATTATATAAGAACAAGATTTTTTTATTTGAGTTTACTCTAGACGAAAATTATAGCGAAGCTAAAAAAAATCAAAGTTTAATTGCAAAATATACTCCACCAATTCAGTATGTTCGTGTTCCTTTTAAATCGCTATGA", "aa": "MQENEVYGSAFFDFNEQTLKLYKNKIFLFEFTLDENYSEAKKNQSLIAKYTPPIQYVRVPFKSL", "aa_hexdigest": "3a26315c376143a7079ebb0d91febe03", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7665.6372, "isoelectric_point": 6.168247032165527 }, "id": "GPKLONFDEH_297", "locus": "GCA000006625_00262" }, { "type": "cds", "sequence": "contig_1", "start": 168179, "stop": 168952, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAAAATATTAAAAAACTTATCAATAGGTGTTGTATCAACAATAATTCTTGGTTCAATAACTATATCATTATCTAATTGTTCTTATAACAAAAAAACATTTTCGTCTTCTACAATTACGAATCAAGATACTAAAAATTTAGACATAATAGCAAAAAACATTAAATTTAAATATGATATTTTTGAAAATGAAGATGAGCAAATAATTAAAATTAATAAAATTAAAGTTGTGGGTGACTTGGGGCAAGCTAAAATAATAATTAAAAAAGTTATTATTGAACAAAAGTCAAATAATTTTTTTGTTCAATACCAACTCAGTTTGCATAACCAAACTAAAGAATATACACAAAACTTAGAAATCAATAAGAAGCAAAGTGTTATTGAAACAAAATATATTAATGATGAATTAATAAATAATGATTATCGTCCTTTTGCATTAAAACAATTATTATCTTCACCGCAAAACATAAAAAAAACTAGTGATCTTTCTTTAATAACAATGCAGAAAAATAACCAAGAATTTAATAATTATTTTAATTTTATTCAAAATGAAGATAATTTTAAACAACTACCCTTTAATCAAGCAACGCTCTTAACAAAACAAAACCAACTAACAGATCATCACACTAACTTTAAATTTGAAAAAATTGAAATAGATAAGCAAAACCAAATTGCAACAGTTAGTTTTAGTCATGATTCTGATTCACAAACAATACAAAAAATATTAAATGCGACAAATAAACGTCTTTTGATTAAAAATTTTGATTGA", "aa": "MQKKILKNLSIGVVSTIILGSITISLSNCSYNKKTFSSSTITNQDTKNLDIIAKNIKFKYDIFENEDEQIIKINKIKVVGDLGQAKIIIKKVIIEQKSNNFFVQYQLSLHNQTKEYTQNLEINKKQSVIETKYINDELINNDYRPFALKQLLSSPQNIKKTSDLSLITMQKNNQEFNNYFNFIQNEDNFKQLPFNQATLLTKQNQLTDHHTNFKFEKIEIDKQNQIATVSFSHDSDSQTIQKILNATNKRLLIKNFD", "aa_hexdigest": "81247da86bcc501a700306d7a7658e4c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29864.706000000027, "isoelectric_point": 9.288363075256349 }, "id": "GPKLONFDEH_298", "locus": "GCA000006625_00263" }, { "type": "cds", "sequence": "contig_1", "start": 169079, "stop": 169384, "strand": "-", "frame": 3, "gene": null, "product": "putative protein UU127", "db_xrefs": [ "GO:0005886", "RefSeq:WP_006688845.1", "SO:0001217", "UniParc:UPI0000139305", "UniRef:UniRef100_Q9PR17", "UniRef:UniRef50_Q9PR17", "UniRef:UniRef90_Q9PR17" ], "nt": "ATGAAGATTATTGGGTCAGCTTTTTTAGGAATAGTTTTTTGCATTCTTTTGGCTTTTGCAATTATTTTTGGAATCGAAATTGATTATTATCATCAAGGTGATTATTTGAAATATCTTAATTTTTTAGATAAATTACACCAATATAATAAAATTGATAATTCATTTGAATACTCAAATCATTATGAATCAGCATTAATTGGTGTAATTGTTTTAACAATTATTTGCTTTTTAATATTCATAACACCAATTATAATAATAGTAATAACAAAGATTAAAGAAAAGAAAGTAATTAACAAAAAGATTTAA", "aa": "MKIIGSAFLGIVFCILLAFAIIFGIEIDYYHQGDYLKYLNFLDKLHQYNKIDNSFEYSNHYESALIGVIVLTIICFLIFITPIIIIVITKIKEKKVINKKI", "aa_hexdigest": "5235241beb0539541d1f19c11bfe5153", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000139305", "ncbi_nrp_id": "WP_006688845.1", "uniref100_id": "UniRef100_Q9PR17", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139305", "RefSeq:WP_006688845.1", "UniRef:UniRef100_Q9PR17" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR17", "uniref90_id": "UniRef90_Q9PR17" }, "psc": { "uniref90_id": "UniRef90_Q9PR17", "product": "Uncharacterized protein UU127", "uniref50_id": "UniRef50_Q9PR17", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR17" ], "product": "Uncharacterized protein UU127" }, "genes": [], "id": "GPKLONFDEH_299", "locus": "GCA000006625_00264" }, { "type": "cds", "sequence": "contig_1", "start": 169527, "stop": 169937, "strand": "-", "frame": 2, "gene": "atpC", "product": "ATP synthase F1 subunit epsilon", "db_xrefs": [ "COG:C", "COG:COG0355", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_006688897.1", "SO:0001217", "UniParc:UPI0000126524", "UniRef:UniRef100_Q9PR16", "UniRef:UniRef50_Q9PR16", "UniRef:UniRef90_Q9PR16" ], "nt": "ATGGCTAATTTAACTAAATTAAAAATTGTTACACCTTATGCACAAATTTTAGAAAAAGATGTGTATAGTGTTGAATTAAAAACATCAGAAGGACGAATTACCGTTTTACCTGACCATAACCCTTTGATGTCAACAATTGAAAACCATGTTGCTTATATTCGTGAATTACCAAATACTCCACGTAAACCACTATTATTATTAGATGGTATAGTTTATATTGAACAACATCAAGTTCGTGTTTTTAGTGACTATTTTAAATTTTTAGATGAAATTCAAATTGATGAAATTAATTCTTCGTTAAATCAATTAAAACATGATTTAAATAATGAAGAAGATGATAAGAAAAAACTTCAATTAAAATCTAAAATTAAATTAAACGAATCAATTTTAATCGCTTATAAAGATCGATAA", "aa": "MANLTKLKIVTPYAQILEKDVYSVELKTSEGRITVLPDHNPLMSTIENHVAYIRELPNTPRKPLLLLDGIVYIEQHQVRVFSDYFKFLDEIQIDEINSSLNQLKHDLNNEEDDKKKLQLKSKIKLNESILIAYKDR", "aa_hexdigest": "5dc40439f496b8b7880540b377fbe853", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI0000126524", "ncbi_nrp_id": "WP_006688897.1", "uniref100_id": "UniRef100_Q9PR16", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000126524", "RefSeq:WP_006688897.1", "UniRef:UniRef100_Q9PR16" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR16", "uniref90_id": "UniRef90_Q9PR16" }, "psc": { "uniref90_id": "UniRef90_Q9PR16", "gene": "atpC", "product": "ATP synthase F1 subunit epsilon", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR16", "cog_id": "COG0355", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR16" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "GPKLONFDEH_300", "locus": "GCA000006625_00265" }, { "type": "cds", "sequence": "contig_1", "start": 169937, "stop": 171256, "strand": "-", "frame": 3, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961", "SO:0001217", "UniRef:UniRef50_P0A300", "UniRef:UniRef90_Q9PR15" ], "nt": "TTGCCTGAAATTAACACTGCTTTAGAATTAAACAATCATGGTTCTAAATTAGTACTTGAGGTTTCTCAATTAGTTGGTGATAATATTGCAAGATGTATTGCAATGGATACAACTGATGGACTAGTACGTGGACAAGAGGTAATTAATACTCAAAAACCAATCACAATGCCTGTTGGTAAACAAGTACTTGGACGTATGTTCAATGTAACTGGTGATCCAATTGATGAACAACCTGCACCAACAGGTAAACGTATGCCAATTCATCGTCCTGCACCTTCATTCGCCGAACAAGCAGAATCAATTGAAATCCTAGAAACGGGAATTAAAGTTGTCGACTTACTTGTACCTTTTGCAAAGGGTGGTAAGATCGGTTTATTTGGTGGTGCTGGAGTTGGTAAAACAGTTTTAATGCAAGAATTAATTCATAACATCGCTAAAAATCATGGTGGTTTATCAGTTTTTGCTGGAGTTGGAGAAAGAACACGTGAAGGAAATGATTTATACTATGAAATGGCTGAGTCAGATGTTTTAGATAAAACAGCTCTTGTCTTTGGACAAATGAATGAACCACCAGGAGCACGTATGCGCGTCGCATTAAGCGGTTTAACAATGGCTGAAGAATTCCGTGATGCTTTTGGTCAAGATGTTTTATTATTTATCGATAACATTTTTAGATTCACACAAGCTGGTTCAGAAGTTTCTGCTTTACTTGGACGAATGCCATCAGCAGTTGGTTATCAACCAACACTTGCTTTTGAAATGGGTCAATTACAAGAACGAATTACATCAACTAAAAAAGGATCAATTACATCGGTTCAAGCTGTTTATGTTCCAGCTGATGACTTAACTGACCCCGCACCAGCAACAACTTTTTCACACTTAGATGCTAAAGTTGTTTTAGATAGAGCTATTGCCTCATTGGGATTATATCCAGCAATTTCACCACTACAATCAACAAGTCGTTTATTAGATCCATTAGTTGTTGGTGTAAAACACTACAGTGTTGCGCGTCGTGTAATTGAAATTTTACAAAGATTTATGGAATTACAAGATATTATTGCAATTCTTGGAATGGATGAATTAAGCGAAGAAGATCGTCAATTAGTAATGCGCGCACGTAAAGTACGTAACTACTTATCACAGCCAAGCCACGTTGCTGAAAAATTCTCTGGTCAACCTGGATTATCAGTTAAACTTGAAGATACTATTGAAGGATTTAGAAAAATTCTTGATGGTGAATGTGATGATATTCATGAACAACATTTCTTATATGTTGGTAAAATTGATGACGTTTTTGAAAAAGCAGCAAAAAATAAATAA", "aa": "MPEINTALELNNHGSKLVLEVSQLVGDNIARCIAMDTTDGLVRGQEVINTQKPITMPVGKQVLGRMFNVTGDPIDEQPAPTGKRMPIHRPAPSFAEQAESIEILETGIKVVDLLVPFAKGGKIGLFGGAGVGKTVLMQELIHNIAKNHGGLSVFAGVGERTREGNDLYYEMAESDVLDKTALVFGQMNEPPGARMRVALSGLTMAEEFRDAFGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAFEMGQLQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKVVLDRAIASLGLYPAISPLQSTSRLLDPLVVGVKHYSVARRVIEILQRFMELQDIIAILGMDELSEEDRQLVMRARKVRNYLSQPSHVAEKFSGQPGLSVKLEDTIEGFRKILDGECDDIHEQHFLYVGKIDDVFEKAAKNK", "aa_hexdigest": "390d37d1c6d7e8f32c2b105c9ac28b73", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR15", "query_cov": 1.0, "subject_cov": 0.9461206896551724, "identity": 0.998, "score": 852.0, "evalue": 1.13e-311, "valid": true, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P0A300", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P0A300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A300" ], "product": "ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "GPKLONFDEH_301", "locus": "GCA000006625_00266" }, { "type": "cds", "sequence": "contig_1", "start": 171358, "stop": 172218, "strand": "-", "frame": 1, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "RefSeq:WP_006688922.1", "SO:0001217", "UniParc:UPI00000C1BAA", "UniRef:UniRef100_Q9PR14", "UniRef:UniRef50_Q9PR14", "UniRef:UniRef90_Q9PR14" ], "nt": "ATGTCTTTAGATGCAATGAAACGAAAAATTAACTCAGTTCAAACAACTGCTAAGATTACGAATGCAATGAAACTAGTGGCAACTGCAAAATTAAAACGTCAGCGTGATCGTTTAGCTGCAATTAAAGAATATTGTCATGATTACTATGATGTTATTGGGTTATTATTGTCTGTTGTTGATGATGTTGAATTTTTAAAAATTCCAAATGCGAAAGATCGAACTTTATATATCACTATTAATTCAACAATGGGTTTAGCGGGTTCTTATAACTATAATGTTAATAAATTAATTTCTAAAATTGTAAATGAAACTGATATAACATTTACAATTGGAAAAAAAGGACATGACTTTATGCGTTTATCAGGTCGTGTTGATCAAGTTAATACTTATTTGAATTTAAATGATAATGATTTAACATTTGACATGAGTTTACAAATTGCGCGCGAGGCTCTTGAATTATATTCACAAGGTGAAGTTAATAAAATTTGTATTATATATACTAAATTTATTAACGCAATTACTTTTGAAGTAAGTGTTATAGATGTTCTTCCATTCGATAAGACAGCTTTAATTAAAGATCATCTTGCTGAAACTATTGAATTAGCAAAAGATAATATTATTTTTAAACCTAACAAGTTTGAGCTTGTTAAAAAAATTCTACCTACTTATATTGCAACAGTTCTATATGGTTCATTAATTGAATCAAAAATCTCTGAAAATGCATCACGACGCAATGCAATGGATGCAGCTACAAAAAATGCCAAAGCATTGGCTGAAAATTATAAATTAATTTACAATACATTACGCCAAGGTAAAATTACTCGTGAAATTACTGAAATTGTTGCTGGTAGTGATGATTAG", "aa": "MSLDAMKRKINSVQTTAKITNAMKLVATAKLKRQRDRLAAIKEYCHDYYDVIGLLLSVVDDVEFLKIPNAKDRTLYITINSTMGLAGSYNYNVNKLISKIVNETDITFTIGKKGHDFMRLSGRVDQVNTYLNLNDNDLTFDMSLQIAREALELYSQGEVNKICIIYTKFINAITFEVSVIDVLPFDKTALIKDHLAETIELAKDNIIFKPNKFELVKKILPTYIATVLYGSLIESKISENASRRNAMDAATKNAKALAENYKLIYNTLRQGKITREITEIVAGSDD", "aa_hexdigest": "8c50bb71c02373d5a07ca338e4bd0993", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BAA", "ncbi_nrp_id": "WP_006688922.1", "uniref100_id": "UniRef100_Q9PR14", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BAA", "RefSeq:WP_006688922.1", "UniRef:UniRef100_Q9PR14" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR14", "uniref90_id": "UniRef90_Q9PR14" }, "psc": { "uniref90_id": "UniRef90_Q9PR14", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR14", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR14" ], "product": "ATP synthase gamma chain" }, "genes": [ "atpG" ], "id": "GPKLONFDEH_302", "locus": "GCA000006625_00267" }, { "type": "cds", "sequence": "contig_1", "start": 172220, "stop": 172405, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGCCACAAATGATTACTTTTATAATTATTGTTGTTAAACTAGCGACTGTATTTTCACCTAAAAAAAGTGCTGAAGAAAAACAAGCTTATAAACTTGCAAAACTTGAAGTTAAAAATCAAAAAAAGGCTAAAAAATTAAATAATTCTTTACAACAAATAAATTTAAATTTAGAGGCAAACTAG", "aa": "MMPQMITFIIIVVKLATVFSPKKSAEEKQAYKLAKLEVKNQKKAKKLNNSLQQINLNLEAN", "aa_hexdigest": "98b828acec040d16033afa690ad23f43", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6959.289700000001, "isoelectric_point": 10.000158500671386 }, "id": "GPKLONFDEH_303", "locus": "GCA000006625_00268" }, { "type": "cds", "sequence": "contig_1", "start": 172601, "stop": 172747, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAACAATTCACGATCAATTGATTAATGGAAAAACAATGGAGTCATTAATTAATAATACTAATAAAAATAACGATCCAAAATTATATGAAGCTCTTCATTATCATGCGCATAGTAAATTTATTAATCATAATGGAACATGA", "aa": "MYKTIHDQLINGKTMESLINNTNKNNDPKLYEALHYHAHSKFINHNGT", "aa_hexdigest": "efea3345a492ee04653196a8da2a097a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5610.216100000001, "isoelectric_point": 8.126384925842284 }, "id": "GPKLONFDEH_304", "locus": "GCA000006625_00269" }, { "type": "cds", "sequence": "contig_1", "start": 172781, "stop": 172897, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGGTTTATTAAATGAAGGTTTTGAAAATGCAAGAGCATTAGTTAATGATCAAGGTCAAATTATTGGTAAAGTAGCTGATGTTAGTGGTGATAATCATTCTTTAATGTCATGA", "aa": "MVGLLNEGFENARALVNDQGQIIGKVADVSGDNHSLMS", "aa_hexdigest": "103228f423ad7fb9f74dec28f66bb0c3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4000.4290999999994, "isoelectric_point": 4.427268791198731 }, "id": "GPKLONFDEH_305", "locus": "GCA000006625_00270" }, { "type": "cds", "sequence": "contig_1", "start": 173203, "stop": 173388, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCAATTTCATCAAATGAAGCAGAACAATTGTTTGATAATGCTAAAAGTGTTGTTTTCTTTAAAGTAACACCTAAATATCCAGTTGAAAAAGTATTAGTTTATGTCACAAGTCCAGTACAAAAAGTTGTTGGTGAATTTGATTTATTAAAGATTGATGTTAATTCAGTAAACACTTCTTGA", "aa": "MISISSNEAEQLFDNAKSVVFFKVTPKYPVEKVLVYVTSPVQKVVGEFDLLKIDVNSVNTS", "aa_hexdigest": "413a38f8911b41dfdc59b80217a5023c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6792.754400000003, "isoelectric_point": 5.096036338806152 }, "id": "GPKLONFDEH_306", "locus": "GCA000006625_00271" }, { "type": "cds", "sequence": "contig_1", "start": 173434, "stop": 174675, "strand": "-", "frame": 1, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "db_xrefs": [ "COG:COG4933", "COG:K", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961", "SO:0001217", "UniRef:UniRef50_Q9PR12", "UniRef:UniRef90_Q9PR12" ], "nt": "ATGCATCAAGGAAAAGATGTTTTAATTGTTTATGATGATCTAAGCAAACATGCTATTGCTTATCGTACTTTATCCTTATTATTACGTCGTCCGCCTGGTCGTGAAGCATATCCTGGAGATGTTTTTTACTTACACAGTCGTTTATTAGAACGTGCATGCAAACTAAAAGATGAATTAGGGGCAGGATCTATTACTGCTTTACCAATTATTGAAACACAAGCTGGTGATATTTCAGCTTATATTCCAACAAATGTAATTTCAATTACTGATGGGCAAATTTTTATGATGACATCATTATTTAATGCTGGTCAACGTCCTGCAATTGATGCAGGGCAATCAGTTAGTCGGGTAGGTTCTGCTGCACAAATTAAATCAGTTAAACAAACTGGAGCATCATTAAAATTAGAATTAGCTAACTATCGCGAACTTGAAGCATTTAGTCAATTCGGTAGTGATTTGGATGATGAAACTAAACGTATTCTAAAACTTGGTAAAGCAGTTATGGCTGTTATTAAGCAAGCTCCTAATAAACCTTATAATCAAACTGATGAAGCGATCATTTTGTTTACCGTTAAAGAAAAACTTATTCCTCAAGTTCCAGTAGAACGTATTCAAGATTTTAAAGAGTACTTATTAAATTACTTTAAAGGAACAAAACTACGTGCTGATCTTGAAGATAAGAAAGCTTTTGATAAAGAAAATACCCCAGCATTTAGATGTGCTATACAAAAAGCAATTAACAGTTTCTTAAATAATTCACAAGATTTTAAACCTTGTGAAGAAGCTGAACAAACAGCCTTTGATAAATTCTTTAATGAGAATGAATCAATTGTTGTTGATGGTGAAAATGATTTTAATTTTATTAATGAAGAAGTTAGTTTAAAACCTACAACAGAAACATCTGAAGCTGTTCAAATAGAAGAAAAAGTTCAAGATTTCGTTGAACCACAAGAAATTCTTGAAACTAATAAAATGGAAGAAAATCATATTTTTGAAGAAGTTGAGCCAGAAAAAATTATCTGTGAACATCATGAATTTGAAATAGCTGAAAACCAAGAAAAAATCGAAGGTCAACAAGTTTTAGAAGATACAAACCATGAATATTCAATTTATGAAACCGTTGAACAAAGCGGTGAGGTAGATAATGATGAATCTAAAGATGATGATTTAGAAGTCTTAGTACCAGTTGCTGAAATTGAGCATGATGAAGCAATTCTAGATGAACGCGAGAATCGTAATTGA", "aa": "MHQGKDVLIVYDDLSKHAIAYRTLSLLLRRPPGREAYPGDVFYLHSRLLERACKLKDELGAGSITALPIIETQAGDISAYIPTNVISITDGQIFMMTSLFNAGQRPAIDAGQSVSRVGSAAQIKSVKQTGASLKLELANYRELEAFSQFGSDLDDETKRILKLGKAVMAVIKQAPNKPYNQTDEAIILFTVKEKLIPQVPVERIQDFKEYLLNYFKGTKLRADLEDKKAFDKENTPAFRCAIQKAINSFLNNSQDFKPCEEAEQTAFDKFFNENESIVVDGENDFNFINEEVSLKPTTETSEAVQIEEKVQDFVEPQEILETNKMEENHIFEEVEPEKIICEHHEFEIAENQEKIEGQQVLEDTNHEYSIYETVEQSGEVDNDESKDDDLEVLVPVAEIEHDEAILDERENRN", "aa_hexdigest": "803431589ce2d4b4de81bc5275ff8173", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpA" ], "truncated": "both", "pseudogene": { "start": 173131, "stop": 174975, "observations": { "mutation": [ 173205, 173436, 174678 ], "stop": [ 173205, 173436, 174678 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR12", "query_cov": 1.0, "subject_cov": 0.5168961201501877, "identity": 1.0, "score": 783.0, "evalue": 1.85e-279, "gene_start": 1, "gene_end": 413, "reference_start": 251, "reference_end": 663, "reference_sequence": "MTDNKNHSLISDIKSQIKKFSEKALTLEVGNVISLGDGIVLVDGLDNVMLNEIVRFENGVEGMALNLEEDAVGVVLLGDYSNIKEGDRVYRTKRIVEVPVGDVMLGRVVDALGKAVDNKGNIVANKFSVIEKIAPGVMDRKSVHQPLETGILSIDAMFPIGKGQRELIIGDRQTGKTTIAIDAIINQKGRNVNCVYVAIGQKNSTIANVVRELEAHGAMEYTTVVTANASELPALQYIAPFTGVTIAEEWMHQGKDVLIVYDDLSKHAIAYRTLSLLLRRPPGREAYPGDVFYLHSRLLERACKLKDELGAGSITALPIIETQAGDISAYIPTNVISITDGQIFMMTSLFNAGQRPAIDAGQSVSRVGSAAQIKSVKQTGASLKLELANYRELEAFSQFGSDLDDETKRILKLGKAVMAVIKQAPNKPYNQTDEAIILFTVKEKLIPQVPVERIQDFKEYLLNYFKGTKLRADLEDKKAFDKENTPAFRCAIQKAINSFLNNSQDFKPCEEAEQTAFDKFFNENESIVVDGENDFNFINEEVSLKPTTETSEAVQIEEKVQDFVEPQEILETNKMEENHIFEEVEPEKIICEHHEFEIAENQEKIEGQQVLEDTNHEYSIYETVEQSGEVDNDESKDDDLEVLVPVAEIEHDEAILDERENRNWVFSDSAVSEVEKQTIMISISSNEAEQLFDNAKSVVFFKVTPKYPVEKVLVYVTSPVQKVVGEFDLLKIDVNSVNTSWNKYRSSSVISSRKEYLEYFNSHKEAHALLASKVYKYRKPKDLASFNMNKGPSGFTYLK" }, "paralog": false, "identity": 0.995114006514658, "score": 1187.0, "evalue": 0.0, "description": "Internal stop codon at 173205, 173436, 174678. Nonsense mutation around 173205, 173436, 174678.", "psc": { "uniref90_id": "UniRef90_Q9PR12", "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR12", "cog_id": "COG4933", "cog_category": "K", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR12", "EC:7.1.2.2", "UniRef:UniRef50_Q9PR12", "COG:COG4933", "COG:K", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR12" ], "product": "ATP synthase subunit alpha" } }, "id": "GPKLONFDEH_307", "locus": "GCA000006625_00272" }, { "type": "cds", "sequence": "contig_1", "start": 174676, "stop": 175425, "strand": "-", "frame": 1, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGACAGATAATAAAAATCATTCATTAATTAGTGATATAAAATCACAAATTAAGAAATTTTCTGAAAAAGCTTTAACCTTAGAGGTCGGCAATGTAATTTCGTTAGGTGATGGAATTGTATTGGTTGATGGCCTTGATAATGTCATGTTAAATGAAATTGTACGTTTTGAAAATGGTGTTGAAGGAATGGCTTTAAACCTAGAAGAAGATGCTGTTGGTGTTGTTCTTTTAGGTGATTATTCAAATATTAAAGAAGGCGATCGTGTTTATCGTACAAAAAGAATTGTTGAAGTTCCTGTTGGGGATGTAATGTTAGGTCGTGTTGTTGACGCTTTAGGTAAGGCAGTTGATAATAAAGGTAATATTGTTGCAAATAAATTTAGTGTAATTGAAAAAATTGCACCTGGTGTTATGGACCGTAAATCTGTTCATCAACCACTTGAAACCGGAATTTTATCAATTGATGCAATGTTCCCAATTGGTAAGGGCCAAAGAGAATTAATTATTGGTGACCGACAGACTGGAAAAACAACAATTGCAATTGATGCTATTATTAATCAAAAAGGCCGTAATGTAAATTGTGTTTATGTAGCAATTGGTCAAAAAAACTCAACAATCGCAAATGTTGTTCGTGAACTTGAAGCTCATGGAGCAATGGAATATACAACTGTTGTTACAGCTAATGCTTCAGAATTACCTGCTTTGCAATATATTGCTCCCTTTACAGGAGTAACAATTGCTGAAGAGTGA", "aa": "MTDNKNHSLISDIKSQIKKFSEKALTLEVGNVISLGDGIVLVDGLDNVMLNEIVRFENGVEGMALNLEEDAVGVVLLGDYSNIKEGDRVYRTKRIVEVPVGDVMLGRVVDALGKAVDNKGNIVANKFSVIEKIAPGVMDRKSVHQPLETGILSIDAMFPIGKGQRELIIGDRQTGKTTIAIDAIINQKGRNVNCVYVAIGQKNSTIANVVRELEAHGAMEYTTVVTANASELPALQYIAPFTGVTIAEE", "aa_hexdigest": "caa9324e04d5abd7c491d7a0aa7de4f9", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.9839357429718876, "subject_cov": 0.9607843137254902, "identity": 0.5429999999999999, "score": 258.0, "evalue": 2.3e-83, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "GPKLONFDEH_308", "locus": "GCA000006625_00273" }, { "type": "cds", "sequence": "contig_1", "start": 175428, "stop": 175727, "strand": "-", "frame": 2, "gene": "atpH", "product": "F-type ATPase subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "RefSeq:WP_006688956.1", "SO:0001217", "UniParc:UPI00000C1BAC", "UniRef:UniRef100_Q9PR11", "UniRef:UniRef50_A0AAP9ABM6", "UniRef:UniRef90_A0AAP9ABM6" ], "nt": "ATGCAAAGTAATACTAAACTAGCAAAATATTCAAAAATTATCGCCAATCGTTTAAAAGCATATTCTAATCAAACTCGTTTTATTGAAATTAAAACTGCTTTTGAATTGAATGATGAACAAAAACAAAGAATTAAAAAAACTGTTATTAGTCGTTTTGGCGATGAAAGACCCATTAAATTTATCGTTGATCCATCATTAATTGGTGGTATATCATTAAAAATCAACTTGGAAATAATTGATTCTTCTCTAAAAACAAAACTAAATCAAATCATCAATACGAAAGAGAAAGAAGGTGCGTAA", "aa": "MQSNTKLAKYSKIIANRLKAYSNQTRFIEIKTAFELNDEQKQRIKKTVISRFGDERPIKFIVDPSLIGGISLKINLEIIDSSLKTKLNQIINTKEKEGA", "aa_hexdigest": "dd81625cb918389fae639d403c5e4b1f", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00000C1BAC", "ncbi_nrp_id": "WP_006688956.1", "uniref100_id": "UniRef100_Q9PR11", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BAC", "RefSeq:WP_006688956.1", "UniRef:UniRef100_Q9PR11" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR11", "uniref90_id": "UniRef90_A0AAP9ABM6" }, "psc": { "uniref90_id": "UniRef90_A0AAP9ABM6", "gene": "AtpH", "product": "F-type ATPase subunit delta", "uniref50_id": "UniRef50_A0AAP9ABM6", "cog_id": "COG0712", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ABM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ABM6" ], "product": "F-type ATPase subunit delta" }, "genes": [ "atpH" ], "id": "GPKLONFDEH_309", "locus": "GCA000006625_00274" }, { "type": "cds", "sequence": "contig_1", "start": 175737, "stop": 176276, "strand": "-", "frame": 2, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_006688889.1", "SO:0001217", "UniParc:UPI00000C1BAD", "UniRef:UniRef100_Q9PR10", "UniRef:UniRef50_Q9PR10", "UniRef:UniRef90_Q9PR10" ], "nt": "ATGAAATCTTCATTAAAACCAGTTGAAAAATACGCTTATTCAATTTTTGAAATTGCTAAAGAAGAAAAAAAATTAGATTTATACAAACATAATTTGGAAACAATTAATTCTATCATTGAAGAGGTTCCAGCTTTTTTTGAAGCTGTTGGTGATCCTGCTCGTGATCGTAATGAAAGAAAACAAATTGTGATTAAAAATCTAGAAGGTGAGATTGATATTTATTTAATTAGTTTAATTGATCTACTAATTGATGTTAAATCGGTTAAACTACTTAAAAAAATAGTTTTAAAAGCTTTGGATTTTGTTAATGAAGCTTTAAGTGTTAAAAAAGTTTTAATAACAACTGCTTATGAATTAACAAAAAATCAAATTGATCGCCTTCTGCAATCTTTAAAAAAGAAATATGTGTGTGAAAAAATAGAACCAATTGTTGTTGTTGATAAATCAATTATTGGAGGACTTTCAATTAATTTTGAATCGCAAGTTTTAGATAATAGTTTAAAAACTAAATTATTTAATATTGTAAAAAAGGTAAATTAG", "aa": "MKSSLKPVEKYAYSIFEIAKEEKKLDLYKHNLETINSIIEEVPAFFEAVGDPARDRNERKQIVIKNLEGEIDIYLISLIDLLIDVKSVKLLKKIVLKALDFVNEALSVKKVLITTAYELTKNQIDRLLQSLKKKYVCEKIEPIVVVDKSIIGGLSINFESQVLDNSLKTKLFNIVKKVN", "aa_hexdigest": "bd4b157a59989a77dc7267f934dc0c59", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1BAD", "ncbi_nrp_id": "WP_006688889.1", "uniref100_id": "UniRef100_Q9PR10", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BAD", "RefSeq:WP_006688889.1", "UniRef:UniRef100_Q9PR10" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR10", "uniref90_id": "UniRef90_Q9PR10" }, "psc": { "uniref90_id": "UniRef90_Q9PR10", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR10", "cog_id": "COG0712", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR10" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "GPKLONFDEH_310", "locus": "GCA000006625_00275" }, { "type": "cds", "sequence": "contig_1", "start": 176280, "stop": 176672, "strand": "-", "frame": 2, "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "db_xrefs": [ "COG:C", "COG:COG0711", "EC:7.1.2.2", "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961", "SO:0001217", "UniParc:UPI000169D88C", "UniRef:UniRef100_Q9PR09", "UniRef:UniRef50_Q9PR09", "UniRef:UniRef90_Q9PR09" ], "nt": "ATGCTAGATAAAAGACGTGAATATATTGCTAAAGAAATTACAGATGCAGAAAATGCTAAACAAGAAGCTTTACAATATTTAGAAAACGCTAAATCTGAACATTTAGCAGCACAAGCACAAACGTTAGAAATTATTGCAAAAGCTAAAAGTGAATCATTAACTTTACGTGAATCATTAGAAAAAGAAGCACGTGAAGCTGCTGATAAAATTATTAGTTCTGCTAAAATTAGCATTGCTAATGAACGTAGAGAAAATCTTGAACGTTTACAATCTGAAGCTCGTGAAGCTGCTTATATTGCAGCTGAAGCATTGATGAAAAAAGAATTGTCTAGAGAAGATAATGATAAACTAGTTGATCAATTCATTAAAGAATTAGAAAATAATGAAAAATAA", "aa": "MLDKRREYIAKEITDAENAKQEALQYLENAKSEHLAAQAQTLEIIAKAKSESLTLRESLEKEAREAADKIISSAKISIANERRENLERLQSEAREAAYIAAEALMKKELSREDNDKLVDQFIKELENNEK", "aa_hexdigest": "40f8c0c9a2f0f568e69c1a277066cff8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000169D88C", "uniref100_id": "UniRef100_Q9PR09", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169D88C", "UniRef:UniRef100_Q9PR09" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR09", "uniref90_id": "UniRef90_Q9PR09" }, "psc": { "uniref90_id": "UniRef90_Q9PR09", "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR09", "cog_id": "COG0711", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR09" ], "product": "ATP synthase subunit b" }, "genes": [ "atpF" ], "id": "GPKLONFDEH_311", "locus": "GCA000006625_00276" }, { "type": "cds", "sequence": "contig_1", "start": 176751, "stop": 176897, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAATAAAAAGCATTTAGTAGCTATATTATCCGTTCTTTCTTTATCAATTATAGTTGTACCACTGTTGACTTCATGTACTGGTGATATTCCAGAATTAAATCCAGCAGAGATTATTAATACACTATTCCCTAATGTATGA", "aa": "MKLNKKHLVAILSVLSLSIIVVPLLTSCTGDIPELNPAEIINTLFPNV", "aa_hexdigest": "85632efc15d7b7ddbf9b4c9e57b0ba7e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5183.217799999999, "isoelectric_point": 6.511156654357909 }, "id": "GPKLONFDEH_312", "locus": "GCA000006625_00277" }, { "type": "cds", "sequence": "contig_1", "start": 176966, "stop": 177295, "strand": "-", "frame": 3, "gene": "atpE", "product": "ATP synthase subunit c", "db_xrefs": [ "COG:C", "COG:COG0636", "GO:0005886", "GO:0008289", "GO:0045263", "GO:0046933", "RefSeq:WP_006688882.1", "SO:0001217", "UniParc:UPI00001265E0", "UniRef:UniRef100_Q9PR08", "UniRef:UniRef50_Q9PR08", "UniRef:UniRef90_Q9PR08" ], "nt": "ATGTCATCATTTATTGATATTACAAATGTTATTTCATCACATATTGAAGCTAATTTACCAGCAATTGCTTCTGAAAATGTAGGTTCACTTGCAAACGGTGCAGGAATTGCTTACTTAGGTAAATATATTGGTACAGGAATTACAATGTTAGCAGCTGGTGCAGTTGGTTTAATGCAAGGTTTTTCTACAGCTAATGCTGTACAAGCTGTAGCTCGTAACCCAGAAGCACAACCAAAGATTTTAAGTACAATGATTGTTGGTTTAGCATTGGCTGAAGCTGTTGCTATTTATGCACTTATTGTTTCTATCTTAATTATTTTCGTTGCTTAA", "aa": "MSSFIDITNVISSHIEANLPAIASENVGSLANGAGIAYLGKYIGTGITMLAAGAVGLMQGFSTANAVQAVARNPEAQPKILSTMIVGLALAEAVAIYALIVSILIIFVA", "aa_hexdigest": "abba82d7058f4834116cb1584b5ec605", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00001265E0", "ncbi_nrp_id": "WP_006688882.1", "uniref100_id": "UniRef100_Q9PR08", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001265E0", "RefSeq:WP_006688882.1", "UniRef:UniRef100_Q9PR08" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR08", "uniref90_id": "UniRef90_Q9PR08" }, "psc": { "uniref90_id": "UniRef90_Q9PR08", "gene": "atpE", "product": "ATP synthase subunit c", "uniref50_id": "UniRef50_Q9PR08", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045263", "GO:0008289", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR08" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "GPKLONFDEH_313", "locus": "GCA000006625_00278" }, { "type": "cds", "sequence": "contig_1", "start": 177677, "stop": 178171, "strand": "-", "frame": 3, "gene": "atpB", "product": "F0F1 ATP synthase subunit A", "db_xrefs": [ "COG:C", "COG:COG0356", "EC:7.1.2.2", "KEGG:K02108", "SO:0001217", "UniRef:UniRef50_Q9PR07", "UniRef:UniRef90_Q9PR07" ], "nt": "GTGGAAAATTATAGCCCGCTTGATATAATGATTGCACTTCCACATATTGCTGCAATCATTATGGTAACTTTAATTATTGGAACAGTTTCGTTGATTTATTTTAGTATGGTTAAAAAGTTAACTGTACATGATGTGCCAAACCGTTTTGTAATTATTATTGGAATGATTGTTGATTATTTTAGAGGACTAGTTGTTGATACAATGGGCGCTAAACATGTTAAATTAGCACCTTATGTACTATTTACATTTTGTTATATTTTTACAGCTAACTTAGTTTCTTTATTTGGTTTCAAAGAAGCAACAACTGCATCTGCCGTTCCATTAGCAATGGCTCTTGGAACCGTGATCGGTGGTCAAATTGTTGCCTTAAAATACCAAAAAGCTTCATTCTTTTTAAAATTTGCTTTTAAAATTAAAGGTTTTCCAATTATGATAAACCCTTTGGAAATCGTTTCTAAATTAACCCCTATTATTTCATTAACATTCCGTTTATGA", "aa": "MENYSPLDIMIALPHIAAIIMVTLIIGTVSLIYFSMVKKLTVHDVPNRFVIIIGMIVDYFRGLVVDTMGAKHVKLAPYVLFTFCYIFTANLVSLFGFKEATTASAVPLAMALGTVIGGQIVALKYQKASFFLKFAFKIKGFPIMINPLEIVSKLTPIISLTFRL", "aa_hexdigest": "b398aabdad273e57f5e9b2e2c6590aa3", "start_type": "GTG", "rbs_motif": "GGAGG", "genes": [ "atpB" ], "truncated": "5-prime", "pseudogene": { "start": 177374, "stop": 178171, "observations": { "mutation": [ 177484, 177601, 177634, 177679 ], "stop": [ 177484, 177601, 177634, 177679 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR07", "query_cov": 1.0, "subject_cov": 0.5963636363636363, "identity": 0.9329999999999999, "score": 295.0, "evalue": 6.1e-99, "gene_start": 1, "gene_end": 164, "reference_start": 1, "reference_end": 164, "reference_sequence": "MENYNPLDIMIALPHIAAIIIVTLIIATISLIYFSMIRKLTVHDVPNRFVIIIGMIVDYFRGLVVDTMGAKHVKLAPYVLFTFCYIFTANLVSLFGFKEATTASSVPLAMALATVVGGQIVALKYQKASFFLKFTFKIKGFPIMVNPLEIVSKLTPIISLTFRLWGNISAAAILLNITYWAFAGFTNVVPWVGVSLIAAVIILPILIGYFTCFAGTIQAFVFTLLTSINWGLEIKEGEEHYAHLAHKKAEKLAAKKLAELDAQNQAQNNEVQVVL" }, "paralog": false, "identity": 0.9283018867924528, "score": 478.0, "evalue": 7.34e-174, "description": "Internal stop codon at 177484, 177601, 177634, 177679. Nonsense mutation around 177484, 177601, 177634, 177679.", "psc": { "uniref90_id": "UniRef90_Q9PR07", "gene": "AtpB", "product": "F0F1 ATP synthase subunit A", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q9PR07", "cog_id": "COG0356", "cog_category": "C", "kegg_orthology_id": "K02108", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR07", "EC:7.1.2.2", "UniRef:UniRef50_Q9PR07", "COG:COG0356", "COG:C", "KEGG:K02108" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR07" ], "product": "ATP synthase A chain" } }, "id": "GPKLONFDEH_314", "locus": "GCA000006625_00279" }, { "type": "cds", "sequence": "contig_1", "start": 178197, "stop": 178526, "strand": "-", "frame": 2, "gene": null, "product": "Conserved hypothetical-cytochrome B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY63", "UniRef:UniRef90_A0A2C9DY63" ], "nt": "TTGTTAGTTATTCCTTTTGGATTTGGAATTACACTTTTACGAATTTTCATTATTTCTAAATTATTAAATAAGCGTTTAAAAATGAATAATTTAGGTGTCAATCCCAAAAAATATCGTCGATTACCAGCAAATTTTTTTGCTTTTGGCACTATTTACATTTTATTTAATTTTCTAATATTAGTGCCTTTGATCATTGGTAATCTTGTTAACCTTTATCAATCTATTTTTGAGACCATCTCATTATTTGTTTTAACATTTTTTATGTTTTTTATCCAAATGGGGATGCAAATTTATTTAGGAATTAAAAAAGATAAAGGAGAAAAAAAATAA", "aa": "MLVIPFGFGITLLRIFIISKLLNKRLKMNNLGVNPKKYRRLPANFFAFGTIYILFNFLILVPLIIGNLVNLYQSIFETISLFVLTFFMFFIQMGMQIYLGIKKDKGEKK", "aa_hexdigest": "4024215d580af1aa2aa8e8141e9e2a9c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 178197, "stop": 178652, "observations": { "mutation": [ 178547 ], "stop": [ 178547 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DY63", "query_cov": 1.0, "subject_cov": 0.7218543046357616, "identity": 0.991, "score": 204.0, "evalue": 6.21e-66, "gene_start": 1, "gene_end": 109, "reference_start": 43, "reference_end": 151, "reference_sequence": "MNLKRMINSYLIIFLPFLSVSFVVFVFLGILLEASWSFFYSYLLVIPFGFGITLLRIFIISKLLNKRLKMNNLGVNPKKYRRLPANFFAFGTIYILFNFLILVPLIIGNLVNLYQSIFETISLFVLTFFMFFIQMGMQIYLGIKKDKGEKK" }, "paralog": false, "identity": 0.9867549668874173, "score": 290.0, "evalue": 2.71e-102, "description": "Internal stop codon at 178547. Nonsense mutation around 178547.", "psc": { "uniref90_id": "UniRef90_A0A2C9DY63", "product": "Conserved hypothetical-cytochrome B", "uniref50_id": "UniRef50_A0A2C9DY63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DY63", "UniRef:UniRef50_A0A2C9DY63" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DY63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY63" ], "product": "Conserved hypothetical-cytochrome B" } }, "id": "GPKLONFDEH_315", "locus": "GCA000006625_00280" }, { "type": "cds", "sequence": "contig_1", "start": 178869, "stop": 179228, "strand": "+", "frame": 3, "gene": null, "product": "putative protein UU139", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR05", "UniRef:UniRef90_Q9PR05" ], "nt": "ATGGATGAACAAATTTTTATTCAATTATTAGAAAACAATCTAACAAATTCAAAATCATATGTGACTCAACCAATAATATTAGAATTTAAAACGGATGTTTTTTTGATTATAAATCAAATTTTAAAACAATATAAAAAAGGTAAAATTATCAAAAATAATACAAATGAAATTATTTTAGAAATTGATAATAAATTAATTTCTATAACAAATTTAGATATCAATAAATTTCATTCGTATCAAATTGTTAATATTAATGAAAATAAAAATATTTATAACGAAAGGAATAGTTTAATTTTATTTGATTTAGTAAATTATATCATTGAAAAAAATCAAAATTCTATCAGACACATTAATTTTTGA", "aa": "MDEQIFIQLLENNLTNSKSYVTQPIILEFKTDVFLIINQILKQYKKGKIIKNNTNEIILEIDNKLISITNLDINKFHSYQIVNINENKNIYNERNSLILFDLVNYIIEKNQNSIRHINF", "aa_hexdigest": "812abe4bbe31cbb33079986e8e5836dc", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 178869, "stop": 179531, "observations": { "mutation": [ 179226, 179253, 179307 ], "stop": [ 179226, 179253, 179307 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PR05", "query_cov": 1.0, "subject_cov": 0.5042372881355932, "identity": 1.0, "score": 165.0, "evalue": 2.96e-49, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MDEQIFIQLLENNLTNSKSYVTQPIILEFKTDVFLIINQILKQYKKGKIIKNNTNEIILEIDNKLISITNLDINKFHSYQIVNINENKNIYNERNSLILFDLVNYIIEKNQNSIRHINFWTIQMSVVNWIYFITYNFNNSYLIDPNWKKYVFKIKKDESKGVISTNLLHNYGFVDFVVQSKSQNFLKAYRVQDEILKSVLNLGDNLNNEFLEEIMRKYDTYKIIDRDHKYLVINIE" }, "paralog": false, "identity": 0.9863636363636363, "score": 357.0, "evalue": 3.87e-127, "description": "Internal stop codon at 179226, 179253, 179307. Nonsense mutation around 179226, 179253, 179307.", "psc": { "uniref90_id": "UniRef90_Q9PR05", "product": "Uncharacterized protein UU139", "uniref50_id": "UniRef50_Q9PR05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PR05", "UniRef:UniRef50_Q9PR05" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR05" ], "product": "Uncharacterized protein UU139" } }, "id": "GPKLONFDEH_316", "locus": "GCA000006625_00281" }, { "type": "cds", "sequence": "contig_1", "start": 179388, "stop": 179579, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTTGTTGTACAGTCTAAATCACAGAATTTTTTAAAAGCTTATCGTGTTCAAGATGAAATTCTAAAATCTGTTTTAAATTTAGGCGATAACTTAAATAATGAATTTTTAGAAGAAATCATGAGAAAATATGATACATACAAAATAATTGATAGAGATCATAAATATTTAGTGATCAATATTGAATAA", "aa": "MDFVVQSKSQNFLKAYRVQDEILKSVLNLGDNLNNEFLEEIMRKYDTYKIIDRDHKYLVINIE", "aa_hexdigest": "0074bf060c1f30ff2968a4a93c3605ca", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7611.6181, "isoelectric_point": 5.287413215637206 }, "id": "GPKLONFDEH_317", "locus": "GCA000006625_00282" }, { "type": "cds", "sequence": "contig_1", "start": 179641, "stop": 179781, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13175.12", "PFAM:PF13476.12", "PFAM:PF13555.12", "PFAM:PF02463.25" ], "nt": "ATGATTTTTTTAAAGAAAATAGAAGCACATGGGTTTAAATCATTTGGGGAGCCAGTTGTTATTGAATTTAAACACCCAATGACTGGAATTATTGGTGCAAATGGAACAGGAAAATCAAACATTATTGATGCTCTTAAATGA", "aa": "MIFLKKIEAHGFKSFGEPVVIEFKHPMTGIIGANGTGKSNIIDALK", "aa_hexdigest": "7bd4f6f5717002d627d8608e824c1503", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13175.12", "name": "AAA_15", "length": 42, "aa_cov": 0.9130434782608695, "hmm_cov": 0.9761904761904762, "evalue": 2.30971169062653e-09, "score": 32.88373565673828, "start": 4, "stop": 45 }, { "id": "PF13476.12", "name": "AAA_23", "length": 41, "aa_cov": 0.8913043478260869, "hmm_cov": 0.975609756097561, "evalue": 3.9264251139121085e-13, "score": 46.05535888671875, "start": 6, "stop": 46 }, { "id": "PF13555.12", "name": "AAA_29", "length": 42, "aa_cov": 0.8913043478260869, "hmm_cov": 0.9761904761904762, "evalue": 9.308084313841938e-08, "score": 27.375383377075195, "start": 5, "stop": 45 }, { "id": "PF02463.25", "name": "SMC_N", "length": 44, "aa_cov": 0.9347826086956522, "hmm_cov": 1.0, "evalue": 7.0610487491829926e-15, "score": 50.600250244140625, "start": 3, "stop": 45 } ], "seq_stats": { "molecular_weight": 4985.864200000001, "isoelectric_point": 9.304093360900879 }, "id": "GPKLONFDEH_318", "locus": "GCA000006625_00283" }, { "type": "cds", "sequence": "contig_1", "start": 179809, "stop": 180087, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGCTCATAAAAATGATTTATTGTTTAGTGGTGGAAGATATGCACCGAAAGCCCATATTGCACGTGTTAATTTATATTTTAATAATGTTAATAATGTTTTATATACACAACATAAAGAAATTAAAATTTCACGAGTGTTGAATATTAAAACAAACGAAAATACTTATTATATTAATGATGAAGTTGCTCTTTTAAAAGATATAACAGATATGTTTTTAGATAGTGGATTATCAAAAGGTTCTTTAGGAATTATTAGTCAAGGTGCAGTGTCATGA", "aa": "MRAHKNDLLFSGGRYAPKAHIARVNLYFNNVNNVLYTQHKEIKISRVLNIKTNENTYYINDEVALLKDITDMFLDSGLSKGSLGIISQGAVS", "aa_hexdigest": "978daa1f22b254ead2b6a1f86e3838e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10342.694799999996, "isoelectric_point": 9.300612068176267 }, "id": "GPKLONFDEH_319", "locus": "GCA000006625_00284" }, { "type": "cds", "sequence": "contig_1", "start": 180121, "stop": 180354, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAGAGGCCTCTGGAATCGGGCGCTATTCAAAGCGAAAGCAAGAAGCGTTAAATTCACTAAGTCGTGCAAACGAAAATTTAGCTCGTTTAAATGACATAGTTGCTAATTTAAAAAAAGAATTGGCGAAATTACAAAAGCAAGCAATGCGTTTTAATGAATATAAACAAATTAAGGACGAATTAACAAAATTAGATTTAGTGATTATAGTACGTGATATCATTCACTGA", "aa": "MFEEASGIGRYSKRKQEALNSLSRANENLARLNDIVANLKKELAKLQKQAMRFNEYKQIKDELTKLDLVIIVRDIIH", "aa_hexdigest": "32bd3a094b1b77a2c467e4aa0ecd0c8e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8972.318699999996, "isoelectric_point": 9.57021884918213 }, "id": "GPKLONFDEH_320", "locus": "GCA000006625_00285" }, { "type": "cds", "sequence": "contig_1", "start": 180364, "stop": 180780, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATATTCAAGAAAATTTAAAAGAATATAAAAAAGAATATAATAAACAAAAACCATTAATTCAAAGTAAAAAAAATGAAATTGAAAGTTTACGAGTAATTTCAGATAGATATGCAAAAGAATACACTAATTTAGAATTTGAATATAAAAAATTAGAAGATACAATAAACGATTTGAATCTTAAATTATCTCTAACTAAAAATACACTTGAAACAACCGCTTTTAATGCAACAAATACACAAGAAAGGATTGATGCTTTTGCTACTTTAATTAAAACAACGGAAATAGAATTAAATAATAATAAAAAAAATATTAATGAAAAAAAAGATCAACTTAAAGATATTCAAAAACAAAAATTAGAACTTGAATCTCTTAAAACAAAACAAAAAAATAATTTGGATACCATCATTTGA", "aa": "MNDIQENLKEYKKEYNKQKPLIQSKKNEIESLRVISDRYAKEYTNLEFEYKKLEDTINDLNLKLSLTKNTLETTAFNATNTQERIDAFATLIKTTEIELNNNKKNINEKKDQLKDIQKQKLELESLKTKQKNNLDTII", "aa_hexdigest": "744cd3707d63ed262409c92eecb2d759", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16357.362999999985, "isoelectric_point": 8.580177879333494 }, "id": "GPKLONFDEH_321", "locus": "GCA000006625_00286" }, { "type": "cds", "sequence": "contig_1", "start": 180811, "stop": 181455, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF06470.18" ], "nt": "ATGTTAGAAGAGCAATTTAAACGCCAAACTGAGAATGAACGTGGTGTAAAAACAATTATTGATTCACAAAAAGCATTAGTTGGTGTTTATGGAACAGTTGCGCAGAATATTTCTACAAAAACAGAACACGAATTAGCAATAAGTACAGCTTTAGCTAAAGGAATCAATAATATTATTGTTAAAAACAATCAAGCAGCTGAAAATTGTATTAATTTTTTAAAGAAAAATAATGCAGGTGTTGCTACTTTTTTACCGATTGAAAATTTGCAGGCTAAAAGCATTAAACCAGAATTTTATGATGTATTAAAAGAATTAGAGGGGTTTGTAGGAGTTGCTAATGAACTAGTAAAAATTAATGTAAATTATAAAATTTCTATTGATTATCTATTAGGAAATACAATTATTGCTAATAATTTAAAAAACGCTATCATTCTAAGTGAATACACATATAAAAATTATAAAGTAGTCTCATTAGAAGGTGACATTGTTTTTGGTGGTGGTGCCATTTCTGGTGGAAGTAATAGTAAAAAAAATATTATTTTAAATCTAGAAGAAAAAATTAAAGAATTAGATTTAAAAATAAAAAAATTAAATGAAGAACAAATTGAATTAAATAATTTACATAACCAAACAAAAAAGCAATGA", "aa": "MLEEQFKRQTENERGVKTIIDSQKALVGVYGTVAQNISTKTEHELAISTALAKGINNIIVKNNQAAENCINFLKKNNAGVATFLPIENLQAKSIKPEFYDVLKELEGFVGVANELVKINVNYKISIDYLLGNTIIANNLKNAIILSEYTYKNYKVVSLEGDIVFGGGAISGGSNSKKNIILNLEEKIKELDLKIKKLNEEQIELNNLHNQTKKQ", "aa_hexdigest": "bfb473d5e144a857555fca80ea19d390", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF06470.18", "name": "SMC_hinge", "length": 118, "aa_cov": 0.5514018691588785, "hmm_cov": 0.9576271186440678, "evalue": 7.622669034411414e-28, "score": 92.87293243408203, "start": 28, "stop": 145 } ], "seq_stats": { "molecular_weight": 23809.04910000003, "isoelectric_point": 8.42738780975342 }, "id": "GPKLONFDEH_322", "locus": "GCA000006625_00287" }, { "type": "cds", "sequence": "contig_1", "start": 181906, "stop": 182586, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5127C5", "UniRef:UniRef90_UPI001E5127C5", "PFAM:PF02463.25" ], "nt": "ATGAGTGAATCAGCAGCACGTTTAAGAATCGAAAGTTTAAAAATTGACCTTGAAAAATATAATGATATTAATTTAAATGCGTTAGAGGAATACGAAGAAAAACAAGCAGAATATGATTCTAATTTTTTAGAGTATGAAAATTGCAAGCAAGCGGTTGATCAAATTAGTAGTTCAATTCAAGAATTAGATAAAAAAGCTAAAGCTGATTTTATGCGTGTTATTCGTGAAACAAATAAATTAATGCCAGAGATTTTTGAATATTTATTTGGTGGAGGATCTTGTGCAATTAGTCTTAATGATCCTAATAATATTTTAGAATCAGGTGTTGAGATAATTGCTAATCCACCAGGTAAACGTGATGTTCCATTATCTGCACTTTCAGGTGGGGAAAAAACAATGGTAGTATTGGCAGTTTTATTTGCTTTATTAAAAATTGCAAAATTTCCATTAGTTATTTTAGATGAAGCTGAAGCAGCTTTAGATGCAAGTAATGTTGCAAAATTCGGTGCCATTATCGCTAAGTATAGTGATGAAACACAATTTTTAGTTATTACTCACCGCGAAGGAACAATGAAAAGTTGTGGTTATCTTTTAGGTACAACAATGATGAATAACGGAGTAACAACATTACTAGAAGTTGATTTAAATGATACAAAAATAAAATATGAAAGTGAATTATAA", "aa": "MSESAARLRIESLKIDLEKYNDINLNALEEYEEKQAEYDSNFLEYENCKQAVDQISSSIQELDKKAKADFMRVIRETNKLMPEIFEYLFGGGSCAISLNDPNNILESGVEIIANPPGKRDVPLSALSGGEKTMVVLAVLFALLKIAKFPLVILDEAEAALDASNVAKFGAIIAKYSDETQFLVITHREGTMKSCGYLLGTTMMNNGVTTLLEVDLNDTKIKYESEL", "aa_hexdigest": "0c49e3399df805220bcb72dd00c1d1dc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 181606, "stop": 182586, "observations": { "mutation": [ 181621 ], "stop": [ 181621 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E5127C5", "query_cov": 1.0, "subject_cov": 0.5368171021377672, "identity": 1.0, "score": 424.0, "evalue": 1.23e-146, "gene_start": 1, "gene_end": 226, "reference_start": 196, "reference_end": 421, "reference_sequence": "MEVIVKKNIILNLEEKIKELDLKIKKLNEEQIELNNLHNQTKKQWQEYYNSEQIINGILNNLITTNNSLSDNLNNYKSQYEALSATKLSKEEIESKISDNWNEYNKKKNELINLKQKIQTLKANLNTANLNKNELEITYNKELEDFYNLDKNITSCESNIDNLNYSIQNVQTRLVDNYKLSVENAIETYQNQEIDMSESAARLRIESLKIDLEKYNDINLNALEEYEEKQAEYDSNFLEYENCKQAVDQISSSIQELDKKAKADFMRVIRETNKLMPEIFEYLFGGGSCAISLNDPNNILESGVEIIANPPGKRDVPLSALSGGEKTMVVLAVLFALLKIAKFPLVILDEAEAALDASNVAKFGAIIAKYSDETQFLVITHREGTMKSCGYLLGTTMMNNGVTTLLEVDLNDTKIKYESEL" }, "paralog": false, "identity": 0.9969325153374233, "score": 632.0, "evalue": 2.24e-231, "description": "Internal stop codon at 181621. Nonsense mutation around 181621.", "psc": { "uniref90_id": "UniRef90_UPI001E5127C5", "uniref50_id": "UniRef50_UPI001E5127C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E5127C5", "UniRef:UniRef50_UPI001E5127C5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E5127C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5127C5" ] } }, "hypothetical": true, "pfams": [ { "id": "PF02463.25", "name": "SMC_N", "length": 144, "aa_cov": 0.6371681415929203, "hmm_cov": 0.5902777777777778, "evalue": 1.556552442016903e-25, "score": 85.44344329833984, "start": 65, "stop": 208 } ], "seq_stats": { "molecular_weight": 25076.376200000013, "isoelectric_point": 4.591703987121583 }, "id": "GPKLONFDEH_323", "locus": "GCA000006625_00288" }, { "type": "cds", "sequence": "contig_1", "start": 182589, "stop": 182807, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTTTTTAAAAAAATATTCAATAAAATTCTAGGAAAAAAAGACACAGAACAAATATCACAAGAGATTTCACAAAAAAATGAAGAAAATCGTATTTTAAAATTAGAGGATAATTCGATTAATAAATTTAATGAAGGATTAAGAAAATCATCTTCTGCTTTAACTGATGCTATTAACGAATTAGCAGTTAAATATATAGATATTGATGAACGATGA", "aa": "MGFFKKIFNKILGKKDTEQISQEISQKNEENRILKLEDNSINKFNEGLRKSSSALTDAINELAVKYIDIDER", "aa_hexdigest": "45c1dad7f83261b1feabeedd33a3bb9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8348.368799999997, "isoelectric_point": 6.257938957214355 }, "id": "GPKLONFDEH_324", "locus": "GCA000006625_00289" }, { "type": "cds", "sequence": "contig_1", "start": 182892, "stop": 183164, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28" ], "nt": "ATGATTTACCAAAAAGTTAATGATCCTGATTTAATAAAGTCTATTATTATTGATAAAATTTTTATTTATTATATTCAAGATACAGAAATTAATACAGCAATTAATTTAAAAGAAAATCAAACTAATGTAGTTTTGGTGGTTGGGGTTAATGGCGTTGGTAAAACAACATCAATCGCAAAAATCACTAAAAAATTCATTGATGAAAATAAAAAAGTTCTCTTAGTAGCTGGAGATACATTTAGAGCAGGTGCTGTTGAACAATTAAAAGTTTGA", "aa": "MIYQKVNDPDLIKSIIIDKIFIYYIQDTEINTAINLKENQTNVVLVVGVNGVGKTTSIAKITKKFIDENKKVLLVAGDTFRAGAVEQLKV", "aa_hexdigest": "49452ae69b358c9df4fa48f88203efc1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 48, "aa_cov": 0.5333333333333333, "hmm_cov": 1.0, "evalue": 2.043677576228213e-20, "score": 68.81938934326172, "start": 42, "stop": 89 } ], "seq_stats": { "molecular_weight": 10024.627199999995, "isoelectric_point": 8.891689109802247 }, "id": "GPKLONFDEH_325", "locus": "GCA000006625_00290" }, { "type": "cds", "sequence": "contig_1", "start": 183318, "stop": 183644, "strand": "+", "frame": 3, "gene": null, "product": "Cell division protein FTSY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "nt": "ATGAATGAATTAAAAAAAATTCATGATGTAATTCATAAATTTGATGAATACGCTCCGCACGAAACTTTATTAGTTTTAGATGCCACTCAAGGCCAATCAGGAATTAATCAAGCAAAAGCATTTAACGAAGTAACGAAAATTTCAGGGATTATTTTAACTAAAATGGATTCTACAAGTCGTGGGGGAATTGTATTAGCAATCAAAGATGCTTTTAATATACCTGTGAAATTGATTGGTTTAGGCGAAAAATTAGAGGATTTAAGTGTATTTGATTTAGAAATGTATGTTGATAGCATAGTTTTAGGAATGAAATTAGATGTTAAATAA", "aa": "MNELKKIHDVIHKFDEYAPHETLLVLDATQGQSGINQAKAFNEVTKISGIILTKMDSTSRGGIVLAIKDAFNIPVKLIGLGEKLEDLSVFDLEMYVDSIVLGMKLDVK", "aa_hexdigest": "85ac56b7a7f6a13f9182444ab3061336", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PYR8", "query_cov": 0.9814814814814815, "subject_cov": 0.8346456692913385, "identity": 0.66, "score": 135.0, "evalue": 5.08e-39, "valid": false, "product": "Cell division protein FTSY", "uniref50_id": "UniRef50_A0A3B0PYR8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PYR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "product": "Cell division protein FTSY" }, "genes": [], "id": "GPKLONFDEH_326", "locus": "GCA000006625_00291" }, { "type": "cds", "sequence": "contig_1", "start": 183661, "stop": 183933, "strand": "+", "frame": 1, "gene": "ylxM", "product": "putative DNA-binding protein", "db_xrefs": [ "COG:COG2739", "COG:K", "KEGG:K09787", "SO:0001217", "UniRef:UniRef50_Q9PR02", "UniRef:UniRef90_Q9PR02" ], "nt": "TTGATTGCTTTATATGATATTTATCAAGGATTATTAACAACAAAACAATGTGAATATTTTAATTTACATTATTTTAAAGATTTGTCGTTTTCTGAAATTGCTGAACTAAAAGAAATTTCAAAAAGCGCTATTAGTGATTGTTTAAACAAAGTGTGTGACCAATTATTAAAATATGAACAAGCACTTTTAATTTATGAAAAAAACAAAAAGCGTAATGATTTATATACATTAATCAACGATTCTGAATTAGTTAAAAAATTAAAAGATATATAA", "aa": "MIALYDIYQGLLTTKQCEYFNLHYFKDLSFSEIAELKEISKSAISDCLNKVCDQLLKYEQALLIYEKNKKRNDLYTLINDSELVKKLKDI", "aa_hexdigest": "3bda0ff222061b42ac69e5db5ac0977a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR02", "query_cov": 1.0, "subject_cov": 0.9090909090909091, "identity": 0.9890000000000001, "score": 176.0, "evalue": 5.07e-56, "valid": true, "gene": "YlxM", "product": "putative DNA-binding protein", "uniref50_id": "UniRef50_Q9PR02", "cog_id": "COG2739", "cog_category": "K", "kegg_orthology_id": "K09787" }, "pscc": { "uniref50_id": "UniRef50_Q9PR02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR02" ], "product": "UPF0122 protein UU142" }, "genes": [ "ylxM" ], "id": "GPKLONFDEH_327", "locus": "GCA000006625_00292" }, { "type": "cds", "sequence": "contig_1", "start": 184184, "stop": 185008, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATGAAAGTATTAATTTAAATAAAAAAACTATCTACGATGATGATGGAAATGAAAAATACACATATTCGAATTTTGCAGATTATACAGTTGTAATTCCTCAAACAATTAGTCAAAATGATTTAAATAAAGATAAATATCAACCATATTATAATTATAGTAGTGTTAATAACCAAGATACTCCAGGAAAAAATCAACTTCAATATTTTGCTTTTCCTTTTAAATTAAAATTTGAAAAAGTAATGGATTTTTGTTTACAAAAAAATAATATATATAAACACTACAATGCTTTAGGTAGATATGATGATAAAATAAGTGAATTAGATTGGTCAGTAGTTAGTATCGATCTAAAACCAATTTTTGAAGCTTTTAAAAATCAAGATTTAAATAAACCGTTTATTTACAATAATAAAACTTTGTCTCCAGAAGAAACTAGTGTTATAAAATATTTTTTAAGTTTAAAAAATATAAAACCATTAGAGGTTAGTCCACAAACAAGATATGTTAGAAGCAACCAAGATGTTGATTGGTATATTGGCACTTTCCCTCGTTATACAAATACTAACCAAAATTCAATGGGGGTTGGCGAACTACGTTATCGTGAATATAATATACAAAAAATCGATTCTGTAAATACTAATTTTGTCACAGGTGGTAAGGGCGTTTTATATAAATCAGATATACCATATACAACAATTAGTACTGACTATATAGATGCTGCGGGTGGTTCATCTGGAACTAGTTTATATGATGAACAAGGACGATTCGTTGGATCAATTGCAACTGGTAGAACTCCAAGTAAAAATGGTCATCCAACGTGA", "aa": "MEYESINLNKKTIYDDDGNEKYTYSNFADYTVVIPQTISQNDLNKDKYQPYYNYSSVNNQDTPGKNQLQYFAFPFKLKFEKVMDFCLQKNNIYKHYNALGRYDDKISELDWSVVSIDLKPIFEAFKNQDLNKPFIYNNKTLSPEETSVIKYFLSLKNIKPLEVSPQTRYVRSNQDVDWYIGTFPRYTNTNQNSMGVGELRYREYNIQKIDSVNTNFVTGGKGVLYKSDIPYTTISTDYIDAAGGSSGTSLYDEQGRFVGSIATGRTPSKNGHPT", "aa_hexdigest": "debffe87e0bf34e34466d2c1c9c8b918", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31616.738200000043, "isoelectric_point": 6.18114948272705 }, "id": "GPKLONFDEH_328", "locus": "GCA000006625_00293" }, { "type": "cds", "sequence": "contig_1", "start": 185363, "stop": 185935, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCAATTTAATATCATTAACTTTGCATGAAAGAGAATATGCAATTTATAAGCATAATGCTACTAATGGTTTTTATTTATATAACACAATAACAAGTAATAAAGATTTTAATTTAATTAAAAATAATAAAGAGTCATATCATCTAAATTCTTTAATTGCACATTTAGCAATTAAAGATGAAGGATTAAAAAAGGATGAATCTGCTGGTTTCTTCTTAGAAAAAGATTTGAATGATTTTGATAATTTAAATAATTTTAAAGATGAAGATATTGTCTTTAATGTAGATCTAGAATTAGATCCTAATCTAATTTATGAATCACAACTTGTAGATAAAAACATGCGAAGATCGCATGTGATAATTCCTATTTCATATAAAGTAATTAAAAAACAACACATTTTAGAAGATGTTGAATTTAGTTTAAATTATGCTTTAGGTTCTGAAGCTTATGAAAATCACATCTATCAACAAATTAAATCACAATTAAAATTTAATGTCTTTTTAAATGGTTCAAAGATAAAAGTTGAAGTTAAGCCACGCAATGATAATATTAAGCTTTATGATTACGTTTGA", "aa": "MRNLISLTLHEREYAIYKHNATNGFYLYNTITSNKDFNLIKNNKESYHLNSLIAHLAIKDEGLKKDESAGFFLEKDLNDFDNLNNFKDEDIVFNVDLELDPNLIYESQLVDKNMRRSHVIIPISYKVIKKQHILEDVEFSLNYALGSEAYENHIYQQIKSQLKFNVFLNGSKIKVEVKPRNDNIKLYDYV", "aa_hexdigest": "aa2a45eb10f05a877fc631d035092c1a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22384.070800000012, "isoelectric_point": 5.975903892517089 }, "id": "GPKLONFDEH_329", "locus": "GCA000006625_00294" }, { "type": "cds", "sequence": "contig_1", "start": 186074, "stop": 186553, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTTGATTTACATGATTTTCCAAAAGATTTGAAAACATTTATTATTACTCATATTCGTTTTGATGATAATATAACTTCGCTTGGTAAAATTAAAGAAAATAGTTTTGAATACTATCAAAACGACAAGGAATATTTACTAAAATCACTTAAATACTACTTTGATATAAAAGAAAATCAACTATACGGTTCTGCTTGTTTTAATTTTAATAATGATGATTTTAAAATCCTAAAAAATAAAACTTTTGTTTTTAAATATGAAATCGACACTAAAAATAATATTTTAAATAAATATATACCATTAAATAAATATATAAATGTTGATTTTAAAAATCTAGCACAATTTAAAATTGTTAATGTTTTCGATGGATTAAATTATAAACTTGAAAGTATAAAAATAGTTAATAAAAACAGCTTGTTACCTTATAATGATCATGTTAATATTCAAAATGCTAATAATACAAATTTTAGTGTTTGA", "aa": "MIFDLHDFPKDLKTFIITHIRFDDNITSLGKIKENSFEYYQNDKEYLLKSLKYYFDIKENQLYGSACFNFNNDDFKILKNKTFVFKYEIDTKNNILNKYIPLNKYINVDFKNLAQFKIVNVFDGLNYKLESIKIVNKNSLLPYNDHVNIQNANNTNFSV", "aa_hexdigest": "5999f0a781d23e39b4d2f3f31464191e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19001.4157, "isoelectric_point": 8.439765739440919 }, "id": "GPKLONFDEH_330", "locus": "GCA000006625_00295" }, { "type": "cds", "sequence": "contig_1", "start": 186593, "stop": 187804, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGCTCCCCCTTGCTTCTTTTTTAATAATGTGCAAGAACCAAGAAGCACCAAAAACAAACCAAGAAGCACCAAAAACAAATAAAGAAAATGTCTTAGACGATTTTGATGCAAATATAATTAATATAGAAAATATTAGTAAACCTAAATTTATTAATCAAAATAAAACAATTATTAATTTAAAATTAAAAAAATTTAATTTTAAATCACCAAATACTACTATAACCTTAACTTATAAAGATAATAATGGTCACAAATTTACATCAGATCCTTTAAGTATTAATGATGAACAAAATTATGATTTTATTTTTTCTAATTTAACCCCTAATCGTAAATATCAAATTCAAAATTTAACTTTTAATAATCAAAAAAGAACAGACATTTATTTGAAAACCAACCTAAATAATGCTATTTTTTCAATAAAACCAATACCTATTAAAACCAATAATTTTAAAATAAAAGTTTTTAATCAAAATGCATTAATATCTTTTAGCATTCCTAAAAATTCTGATGTAAGAGTAAATGAAAAAATAGCATTAGAATTTGAAAATTTAAGTAGTAATTTAGTGCCTAATAACGAAATAATTAGTAGAATTGATAAAAATTTTAATGTAGAATTTAAATTAGATAATTTAAAATTAAATAATAAATATCGAATTGTAAATCTTAGATTTTTAGATACTAATCCACCTAATGTTAGTCCAAATATTTTTGAAAAGTTATCTAATTATGAAAGCAGTTTTATCATACCAGGTATTAAAACAAATATGCATAATCATAAAGACTTGAATAGTAATGACAAAAAATATATTGATAGTCCTTATAACACTAAAATTGAACTTAATGATAAAAATAATTTTAATGATAAAGTGTTGCCAGATGCATTTGAACAGCAAAACATTAATATACAAGAAGTTGAGAATCTTAATCAAGACATCTTAAATCAAAATGAGCTAGAATTACAAAAATATTTTAGTTTTATTAGTGATCAAGCAAACCTTAAAGACAAAGATTTTCAAAACTATTCTTTTAATGATATTAATAAAAATTCAAAGCAAGAAATTAAATTTAAAATCAAACATATTAACATCGATAGTAGTAATAAAAAAGCAATCATTGAATTAGATTCGTCTAGTTCTAATGATAATACTAAACTTTTAGAAGCAAATAATAAACAATTGTTAATTAAATCATATGATTATAATAATCCCTGA", "aa": "MLPLASFLIMCKNQEAPKTNQEAPKTNKENVLDDFDANIINIENISKPKFINQNKTIINLKLKKFNFKSPNTTITLTYKDNNGHKFTSDPLSINDEQNYDFIFSNLTPNRKYQIQNLTFNNQKRTDIYLKTNLNNAIFSIKPIPIKTNNFKIKVFNQNALISFSIPKNSDVRVNEKIALEFENLSSNLVPNNEIISRIDKNFNVEFKLDNLKLNNKYRIVNLRFLDTNPPNVSPNIFEKLSNYESSFIIPGIKTNMHNHKDLNSNDKKYIDSPYNTKIELNDKNNFNDKVLPDAFEQQNINIQEVENLNQDILNQNELELQKYFSFISDQANLKDKDFQNYSFNDINKNSKQEIKFKIKHINIDSSNKKAIIELDSSSSNDNTKLLEANNKQLLIKSYDYNNP", "aa_hexdigest": "bc55e20af4e8c76beb7ffbae99d590ea", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 56, "aa_cov": 0.13895781637717122, "hmm_cov": 0.9821428571428571, "evalue": 4.989118359040284e-14, "score": 48.07280349731445, "start": 68, "stop": 123 } ], "seq_stats": { "molecular_weight": 46942.30070000001, "isoelectric_point": 8.81574535369873 }, "id": "GPKLONFDEH_331", "locus": "GCA000006625_00296" }, { "type": "tRNA", "sequence": "contig_1", "start": 188044, "stop": 188120, "strand": "+", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 78.5, "nt": "GGAAGTATAGCTCAGTTGGTTAGAGCACACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGCCCACTTATTTCCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 188078, 188080 ], "id": "GPKLONFDEH_2", "locus": "GCA000006625_00297" }, { "type": "tRNA", "sequence": "contig_1", "start": 188124, "stop": 188199, "strand": "+", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 73.2, "nt": "GGGGACGTAGCTCAATTGATAGAGCACCTGATTTGCACTCAGGAGGTCGTGGGTTTGACCCCCATCGTCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 188157, 188159 ], "id": "GPKLONFDEH_3", "locus": "GCA000006625_00298" }, { "type": "cds", "sequence": "contig_1", "start": 188298, "stop": 189029, "strand": "-", "frame": 2, "gene": null, "product": "putative protein UU144", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013935F", "UniRef:UniRef100_Q9PR00", "UniRef:UniRef50_Q9PR00", "UniRef:UniRef90_Q9PR00" ], "nt": "TTGTCGCTCCATAAAACCATATACATAAAGTTTAATAATCGTGATTATGATGTTAAATTGACTAATGTTAAAATTGCAAATATTGCATTAAATAATGATAATGTTAATAATATTGAACAAAATCATATTTCATTTCGACATCTATCAACCAATGATATTAAAAATTCTAATGATAAAGTAGAAGCTAAATTTTCAATTGGATTAGGTTTTGATATATCTGAAAAACTAAAAAATATTGCATTTAAAAAGTTAGACACTAATACTAATAAATTAATTGATATATCTGATCCAACAATTTTACGTTTAACTTTCTACGAATATAATAAAGATCAAAAAGTAGAAAACAATCTTGCTGCACCAACAGATAAAACAAAAATTTCAGTTGTTGAAATTAAAAAATCACAATTAATGAATTCATCTATTGTTTCTTTTGTAAAAAAATTAAACAAAAATGCTAAGTATCATATGGCTAATATCGCCTTTAACCAACAATTATTACCATATTTAAATAAACAATTCAATGTTAATGCTGATTTTCCAATTGATCTAGAGCAAAATCCAATCGACTTTAATGATAATAAACAAGCCAATGATGAAAAAATTAAAAAGACAAAAGAATCTGCTATAAAAGTAGCTACAGCAATTGCTAAACAAATTGTTGGAATAGATTATAAAGGAAGAGACATTATTAAAAATTTTATTGAATTAGATAATGTAGTAAATAAAAAATAA", "aa": "MSLHKTIYIKFNNRDYDVKLTNVKIANIALNNDNVNNIEQNHISFRHLSTNDIKNSNDKVEAKFSIGLGFDISEKLKNIAFKKLDTNTNKLIDISDPTILRLTFYEYNKDQKVENNLAAPTDKTKISVVEIKKSQLMNSSIVSFVKKLNKNAKYHMANIAFNQQLLPYLNKQFNVNADFPIDLEQNPIDFNDNKQANDEKIKKTKESAIKVATAIAKQIVGIDYKGRDIIKNFIELDNVVNKK", "aa_hexdigest": "c2f5b148d670bc8b7da9aebdb8a83a73", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013935F", "uniref100_id": "UniRef100_Q9PR00", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013935F", "UniRef:UniRef100_Q9PR00" ] }, "ips": { "uniref100_id": "UniRef100_Q9PR00", "uniref90_id": "UniRef90_Q9PR00" }, "psc": { "uniref90_id": "UniRef90_Q9PR00", "product": "Uncharacterized protein UU144", "uniref50_id": "UniRef50_Q9PR00" }, "pscc": { "uniref50_id": "UniRef50_Q9PR00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR00" ], "product": "Uncharacterized protein UU144" }, "genes": [], "id": "GPKLONFDEH_332", "locus": "GCA000006625_00299" }, { "type": "cds", "sequence": "contig_1", "start": 189033, "stop": 189224, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAACAAATTTCTACAAAGTATTAAAAAACGTAATTTATCAAAAAATACATATTTAACTTATGAATCACATTTAAGAAAGTTTAAGGTACAAGAACAAAGTTTTAAACAGATTATTAAGCAATTATTAAATAACAATTACAAACCAAAAAGTCTAAAATTAGCTAAGTCAATTTTAAGTGAGTATTGA", "aa": "MINKFLQSIKKRNLSKNTYLTYESHLRKFKVQEQSFKQIIKQLLNNNYKPKSLKLAKSILSEY", "aa_hexdigest": "09384116466758862624b0c78ebe17dd", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7606.886800000002, "isoelectric_point": 10.218190574645995 }, "id": "GPKLONFDEH_333", "locus": "GCA000006625_00300" }, { "type": "cds", "sequence": "contig_1", "start": 189669, "stop": 189800, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAGATTGTACAGGATTTAGTTGGTCATGAAAATATCCAAACAACACTACAATATGTACAAATCACAAAAGAACAAGTGGACGACGTTTATAAAGAATTTTTTAAGGAATTTGAAAATGATAAATAA", "aa": "MLKIVQDLVGHENIQTTLQYVQITKEQVDDVYKEFFKEFENDK", "aa_hexdigest": "73171c218281f65ea7a3475ad870f714", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5204.814299999999, "isoelectric_point": 4.597785758972168 }, "id": "GPKLONFDEH_334", "locus": "GCA000006625_00301" }, { "type": "cds", "sequence": "contig_1", "start": 189790, "stop": 190155, "strand": "+", "frame": 1, "gene": null, "product": "putative protein UU146", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQZ8", "UniRef:UniRef90_Q9PQZ8" ], "nt": "ATGATAAATAATATTTTTAATAAAAAGTTGTTAGTTTACAACGAAAAAGATCAACGTTGGTATAACAAATTTGATAGAACTAAACCTTTATTTTCACCAAGTAGACTATTAAGTTTTAAAAATTACATTTTTGAAAAAATACCAGCAAAAAATCTAGAATATCCAATTGAGTTTGGAACTAGTTTAATGCAAATCATTCAATACTATTTTGAAAATCAATCAAAATTAGATGTCATTTTAAATAGTATTAATGAAGAAAAATTAAAAGAAGTCTTTTTATTGTTTTTAGACTATTTAAAAACAAATGATTATTCACTAATCGCTGTTGAATTACATTTATTTAGTCCAAAACTAAACCATTACTGA", "aa": "MINNIFNKKLLVYNEKDQRWYNKFDRTKPLFSPSRLLSFKNYIFEKIPAKNLEYPIEFGTSLMQIIQYYFENQSKLDVILNSINEEKLKEVFLLFLDYLKTNDYSLIAVELHLFSPKLNHY", "aa_hexdigest": "175ae1b85100ec12614c086aebd43dff", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 189790, "stop": 190416, "observations": { "mutation": [ 190153, 190249 ], "stop": [ 190153, 190249 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQZ8", "query_cov": 1.0, "subject_cov": 0.5817307692307693, "identity": 1.0, "score": 240.0, "evalue": 3.21e-79, "gene_start": 1, "gene_end": 121, "reference_start": 1, "reference_end": 121, "reference_sequence": "MINNIFNKKLLVYNEKDQRWYNKFDRTKPLFSPSRLLSFKNYIFEKIPAKNLEYPIEFGTSLMQIIQYYFENQSKLDVILNSINEEKLKEVFLLFLDYLKTNDYSLIAVELHLFSPKLNHYWHGFADVVVKTKDDKYKVLEIKTHNEIQSVPSWKFQAGVYDYIISQELSNYEPSAILAFSRKQAKLSVYENDSLDLSFINKINELYS" }, "paralog": false, "identity": 0.9903846153846154, "score": 409.0, "evalue": 3.14e-148, "description": "Internal stop codon at 190153, 190249. Nonsense mutation around 190153, 190249.", "psc": { "uniref90_id": "UniRef90_Q9PQZ8", "product": "Uncharacterized protein UU146", "uniref50_id": "UniRef50_Q9PQZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQZ8", "UniRef:UniRef50_Q9PQZ8" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQZ8" ], "product": "Uncharacterized protein UU146" } }, "id": "GPKLONFDEH_335", "locus": "GCA000006625_00302" }, { "type": "cds", "sequence": "contig_1", "start": 190575, "stop": 190916, "strand": "+", "frame": 3, "gene": null, "product": "putative protein UU148", "db_xrefs": [ "RefSeq:WP_004025751.1", "SO:0001217", "UniParc:UPI0000139373", "UniRef:UniRef100_Q9PQZ6", "UniRef:UniRef50_Q9PQZ6", "UniRef:UniRef90_Q9PQZ6" ], "nt": "ATGGATAAAATTAAAAATTATCTAGAAAAATTACAAGCGTTTTATAAGTTATATTCTCTTAATTTAACTCAAGGAACAGAAATTTTAATTATTCAAATCATTGATAAAGGTAATTTAATTTTTGATTCCATTGAAAAAGGTAAAACATTTAACGAAAGTAAAATAATAGAACCACTTTTATTCATTTTTAAAAAAATAAATACAATTTTAGCTATCTTAAAATTAACTTTTACTAAAGATGATTTAATTGGAATTCTACAATCATTAGAAGAGCATATATCAGAATTTATTATTGCAAATTATAAATATGATTTTGAAATCGAAGAAGATGATGAAGATTAA", "aa": "MDKIKNYLEKLQAFYKLYSLNLTQGTEILIIQIIDKGNLIFDSIEKGKTFNESKIIEPLLFIFKKINTILAILKLTFTKDDLIGILQSLEEHISEFIIANYKYDFEIEEDDED", "aa_hexdigest": "90d4f1882f30025151c8cc5164223e86", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0000139373", "ncbi_nrp_id": "WP_004025751.1", "uniref100_id": "UniRef100_Q9PQZ6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139373", "RefSeq:WP_004025751.1", "UniRef:UniRef100_Q9PQZ6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQZ6", "uniref90_id": "UniRef90_Q9PQZ6" }, "psc": { "uniref90_id": "UniRef90_Q9PQZ6", "product": "Uncharacterized protein UU148", "uniref50_id": "UniRef50_Q9PQZ6" }, "pscc": { "uniref50_id": "UniRef50_Q9PQZ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQZ6" ], "product": "Uncharacterized protein UU148" }, "genes": [], "id": "GPKLONFDEH_336", "locus": "GCA000006625_00303" }, { "type": "cds", "sequence": "contig_1", "start": 190922, "stop": 191218, "strand": "+", "frame": 2, "gene": null, "product": "putative protein UU149", "db_xrefs": [ "RefSeq:WP_010891688.1", "SO:0001217", "UniParc:UPI000013937A", "UniRef:UniRef100_Q9PQZ5", "UniRef:UniRef50_Q9PQZ5", "UniRef:UniRef90_Q9PQZ5" ], "nt": "ATGGAAAAAATAGTTGGCTTTATTGTGAACATAATTAATGTTCAAAAACAAATGATAAAAGACTTAAAAGAACTAAAAAATCATAAAGACATCATGGTAAAAGACCATGAAAAATTAAATGATATGTTAAATAGTATGATAGATGAAGCAGTTAAAAATTACAAAATTGCATTAAAAAACATAACAGAAATTCTAAACGTTCATTTAAACTGTGTTTTATATAATCACAATCTTAAATGTGGCGTAAAAGATGAAAAATTATTATTACCACTATTTAAAAGAAAGAAAGCGAGTTAA", "aa": "MEKIVGFIVNIINVQKQMIKDLKELKNHKDIMVKDHEKLNDMLNSMIDEAVKNYKIALKNITEILNVHLNCVLYNHNLKCGVKDEKLLLPLFKRKKAS", "aa_hexdigest": "c6e2a9d0f7b171cee40c078fa129273d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013937A", "ncbi_nrp_id": "WP_010891688.1", "uniref100_id": "UniRef100_Q9PQZ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013937A", "RefSeq:WP_010891688.1", "UniRef:UniRef100_Q9PQZ5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQZ5", "uniref90_id": "UniRef90_Q9PQZ5" }, "psc": { "uniref90_id": "UniRef90_Q9PQZ5", "product": "Uncharacterized protein UU149", "uniref50_id": "UniRef50_Q9PQZ5" }, "pscc": { "uniref50_id": "UniRef50_Q9PQZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQZ5" ], "product": "Uncharacterized protein UU149" }, "genes": [], "id": "GPKLONFDEH_337", "locus": "GCA000006625_00304" }, { "type": "cds", "sequence": "contig_1", "start": 191225, "stop": 191461, "strand": "+", "frame": 2, "gene": null, "product": "putative protein UU150", "db_xrefs": [ "RefSeq:WP_004026929.1", "SO:0001217", "UniParc:UPI0000139387", "UniRef:UniRef100_Q9PQZ4", "UniRef:UniRef50_Q9PQZ4", "UniRef:UniRef90_Q9PQZ4" ], "nt": "ATGGCAATGCTTTGTATGTGTGATGAATGCCAAGCAATTATTAATTATTATGTTGTACAAATTGAACAATTGTGTAACGAAATTGTAAAAACAAGAAAAAGAGTTGAACCATATAAAAAATTGGGTAAATTGTTTTTTGAAATTATTCAAAATGATTTATTTGAAGCAATAGCTGAAGAAGTGTTTAGAATAGATGAAAAACCAAAACGAAAAACAAATAAAAGAGTATGTAAATAA", "aa": "MAMLCMCDECQAIINYYVVQIEQLCNEIVKTRKRVEPYKKLGKLFFEIIQNDLFEAIAEEVFRIDEKPKRKTNKRVCK", "aa_hexdigest": "b5d71b810ec82720e975f7db0261e110", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0000139387", "ncbi_nrp_id": "WP_004026929.1", "uniref100_id": "UniRef100_Q9PQZ4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139387", "RefSeq:WP_004026929.1", "UniRef:UniRef100_Q9PQZ4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQZ4", "uniref90_id": "UniRef90_Q9PQZ4" }, "psc": { "uniref90_id": "UniRef90_Q9PQZ4", "product": "Uncharacterized protein UU150", "uniref50_id": "UniRef50_Q9PQZ4" }, "pscc": { "uniref50_id": "UniRef50_Q9PQZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQZ4" ], "product": "Uncharacterized protein UU150" }, "genes": [], "id": "GPKLONFDEH_338", "locus": "GCA000006625_00305" }, { "type": "cds", "sequence": "contig_1", "start": 191418, "stop": 191600, "strand": "+", "frame": 3, "gene": null, "product": "putative protein UU151", "db_xrefs": [ "RefSeq:WP_004025899.1", "SO:0001217", "UniParc:UPI0000139389", "UniRef:UniRef100_Q9PQZ3", "UniRef:UniRef50_Q9PQZ3", "UniRef:UniRef90_Q9PQZ3" ], "nt": "ATGAAAAACCAAAACGAAAAACAAATAAAAGAGTATGTAAATAAATTTAAAGATTTGCGTAATCAACTAACAAAAAACAATGACGTTAAAAAAGTTTGCGATTTAATTAATGATTTGTTTTTTGAAGTATATAAAAACAATTTAATAGATGAAGTTATCAAGGAGATTAACAAACATGACTAA", "aa": "MKNQNEKQIKEYVNKFKDLRNQLTKNNDVKKVCDLINDLFFEVYKNNLIDEVIKEINKHD", "aa_hexdigest": "367e68100b12e55f9d98b5cba25f1133", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0000139389", "ncbi_nrp_id": "WP_004025899.1", "uniref100_id": "UniRef100_Q9PQZ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139389", "RefSeq:WP_004025899.1", "UniRef:UniRef100_Q9PQZ3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQZ3", "uniref90_id": "UniRef90_Q9PQZ3" }, "psc": { "uniref90_id": "UniRef90_Q9PQZ3", "product": "Uncharacterized protein UU151", "uniref50_id": "UniRef50_Q9PQZ3" }, "pscc": { "uniref50_id": "UniRef50_Q9PQZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQZ3" ], "product": "Uncharacterized protein UU151" }, "genes": [], "id": "GPKLONFDEH_339", "locus": "GCA000006625_00306" }, { "type": "cds", "sequence": "contig_1", "start": 191819, "stop": 192136, "strand": "+", "frame": 2, "gene": null, "product": "putative protein UU153", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQZ1", "UniRef:UniRef90_Q9PQZ1" ], "nt": "ATGACAAATAGTATTAAACGTGAAATATTAAGTGCTTTAAATGATGAAACAAAAAAAGTAATTAGCAAATTACAACAAACAAATAATTTAATTAAACAATTAAGCGATGAAAAAAAGACATTAGAACAATATTTAAAAGATAATGTTAACAATCATGAAATCGTTGATTTAGACAGTGATTTAAAAATTAAAATCTATGATTTAACATCTAAGGATAGTATTGTTTTAGATTATGACAAAATCATTACTGATTATAAAGTTGATACAAAAAAATATGAAACGATTAAAAAAGGAACAACATCAACACGAATAACATGA", "aa": "MTNSIKREILSALNDETKKVISKLQQTNNLIKQLSDEKKTLEQYLKDNVNNHEIVDLDSDLKIKIYDLTSKDSIVLDYDKIITDYKVDTKKYETIKKGTTSTRIT", "aa_hexdigest": "10e73426efb5c9075e17d9ecf0479ec1", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q9PQZ1", "query_cov": 1.0, "subject_cov": 0.9459459459459459, "identity": 1.0, "score": 190.0, "evalue": 6.31e-61, "valid": true, "product": "Uncharacterized protein UU153", "uniref50_id": "UniRef50_Q9PQZ1" }, "pscc": { "uniref50_id": "UniRef50_Q9PQZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQZ1" ], "product": "Uncharacterized protein UU153" }, "genes": [], "id": "GPKLONFDEH_340", "locus": "GCA000006625_00307" }, { "type": "cds", "sequence": "contig_1", "start": 192265, "stop": 192594, "strand": "+", "frame": 1, "gene": "bet", "product": "phage recombination protein Bet", "db_xrefs": [ "GO:0003677", "GO:0006310", "SO:0001217", "UniRef:UniRef50_Q9PQZ0", "UniRef:UniRef90_Q9PQZ0" ], "nt": "TTGACCATTCAAGGAATTGATTTAAAAGCCATTGATTTAAACCAAGCAGCACAAATTATTTATTTTTGCCAAGCAAATAATTTAAATCCACTAAACAAAGAAGTTTATTTAATTCAAATGGGCAATCGTTTAGCACCAATTGTTGGTATTCATACTATGACAGAACGTGCTTATAGAACTGAACGATTAGTTGGTATTGTACAAAGTTATAACGATGTTAACAAAAGTGCTAAGACAATACTAACAATCAGATCGCCAGGTTTAAAAGGTCTTGGAACTGTTGAAGCTGAAGTATTTTTAAGCGAATACAGCACGAATAAAAACTTGTGA", "aa": "MTIQGIDLKAIDLNQAAQIIYFCQANNLNPLNKEVYLIQMGNRLAPIVGIHTMTERAYRTERLVGIVQSYNDVNKSAKTILTIRSPGLKGLGTVEAEVFLSEYSTNKNL", "aa_hexdigest": "cf78706d8ce203659c8899e686f96ccb", "start_type": "TTG", "rbs_motif": null, "genes": [ "bet" ], "truncated": "both", "pseudogene": { "start": 192154, "stop": 192810, "observations": { "mutation": [ 192220, 192592 ], "stop": [ 192220, 192592 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQZ0", "query_cov": 1.0, "subject_cov": 0.4977168949771689, "identity": 0.9359999999999999, "score": 206.0, "evalue": 1.38e-65, "gene_start": 1, "gene_end": 109, "reference_start": 38, "reference_end": 146, "reference_sequence": "MIKDDKLVPSVKLLTPNELSEQWANPNSEINQITRAVLSIQGIDLKAIDLNQAAQIIYFCQANNLNPLNKEVYLIQMGNRLAPIVGIHTMTERAYKTERLVGITQSYNDTNKSAKTILTIRAPGLKGFGTVEAEVFLSEYSTNKNLWVTKPITMLKKVSLAHALRLSGLLAFKGDTPYIYEEMQQGETTPVKKMFTPPVQEVIEPQKIEQIKKVDFNEF" }, "paralog": false, "identity": 0.908675799086758, "score": 389.0, "evalue": 1.71e-140, "description": "Internal stop codon at 192220, 192592. Nonsense mutation around 192220, 192592.", "psc": { "uniref90_id": "UniRef90_Q9PQZ0", "gene": "bet", "product": "phage recombination protein Bet", "uniref50_id": "UniRef50_Q9PQZ0", "go_ids": [ "GO:0003677", "GO:0006310" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQZ0", "UniRef:UniRef50_Q9PQZ0", "GO:0003677", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQZ0" ], "product": "Uncharacterized protein UU154" } }, "id": "GPKLONFDEH_341", "locus": "GCA000006625_00308" }, { "type": "cds", "sequence": "contig_1", "start": 192613, "stop": 192810, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAAAGGTGTCTTTGGCCCACGCATTGCGTTTAAGTGGCTTATTAGCGTTTAAAGGTGATACGCCTTATATTTATGAAGAAATGCAACAAGGCGAAGCAGTGCCAAATAAAAAAATGTTTACACCACCAGTTGCTGAAGTAATTGAACCAGCAGTTGAAAATATAAAGAAAGTAGATTTTAATGAGTTCTAA", "aa": "MLKKVSLAHALRLSGLLAFKGDTPYIYEEMQQGEAVPNKKMFTPPVAEVIEPAVENIKKVDFNEF", "aa_hexdigest": "3d8b8fb29831147b33b00d9a8a3a3881", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7322.479100000002, "isoelectric_point": 5.72024211883545 }, "id": "GPKLONFDEH_342", "locus": "GCA000006625_00309" }, { "type": "cds", "sequence": "contig_1", "start": 193070, "stop": 193225, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGTTGTTTCATTTGATAATATTTTTTACAAGCATCAATTAAGCGAACAACAAACTATTGATTTTGTGAGTGCTGAAAAAGAATATAACTATCAAACACAAACAGTTAATTCTTATAGTAATAGTGATGATGAAATTGAAATTATTAACTGA", "aa": "MRVVSFDNIFYKHQLSEQQTIDFVSAEKEYNYQTQTVNSYSNSDDEIEIIN", "aa_hexdigest": "970873a942b2c4bd7c0273c629ee7788", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6082.496700000001, "isoelectric_point": 4.204403114318848 }, "id": "GPKLONFDEH_343", "locus": "GCA000006625_00310" }, { "type": "cds", "sequence": "contig_1", "start": 193268, "stop": 193489, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTGATTATAAAAACCAAGGCTTTTTTAAAAATAAAACAATGCCAGATAAACTAAAAAATAACGCTAATGCTTTAGAACTTTATAAACAAATTATTAAATTAGATTTAGAAGCATTTAGTGGTTTATACAACGGCTTAAAACCATTTGAAGCAGCAAATAAATTAATTGAAAAAGGCTATATAACACTTAACGATTTCTTTAAATATGCGATCTAA", "aa": "MFIDYKNQGFFKNKTMPDKLKNNANALELYKQIIKLDLEAFSGLYNGLKPFEAANKLIEKGYITLNDFFKYAI", "aa_hexdigest": "4afe85cd09ea4781b7dd2d3d46c9006b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8517.822099999994, "isoelectric_point": 9.156654167175294 }, "id": "GPKLONFDEH_344", "locus": "GCA000006625_00311" }, { "type": "cds", "sequence": "contig_1", "start": 193500, "stop": 193925, "strand": "+", "frame": 3, "gene": null, "product": "putative protein UU156", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQY8", "UniRef:UniRef90_Q9PQY8" ], "nt": "TTGAGCACACAAGATGCTAATTTAGCAAATAAAAAATATGATTATATTTACTTTAGGGGTGTTAATCAACTTTATTTAAAACGTCTTAAAAAAAGTAGTAATAAGTTTTTTGTTTATAAAAAATGTAAAATCGTACCACGTATGATATCAAAACATTTAAATAACGACCATTTAATTAACGTTAATGATTATGTACCACTAACCAAAGAATTTATTAAAAAAAGCGTTGAAAAAGAAGTTAAACATTATCTAAGGTTTATATCTTATGAAAACAAAACTAAACAAATGCCTGAACTAATTAATTTTTTAGAAAAATCACTTGAAGATAAGTTATTTATTGTCGCAAAAGATTATGATCCTTTATTTTCTGTGTTTTTACGTTATTACATTCAAAGTTTAATAGAAAAATATATTAATGAATTATGA", "aa": "MSTQDANLANKKYDYIYFRGVNQLYLKRLKKSSNKFFVYKKCKIVPRMISKHLNNDHLINVNDYVPLTKEFIKKSVEKEVKHYLRFISYENKTKQMPELINFLEKSLEDKLFIVAKDYDPLFSVFLRYYIQSLIEKYINEL", "aa_hexdigest": "495eb6c53ad9cffe1dfde4e4d7b03dbd", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQY8", "query_cov": 1.0, "subject_cov": 0.9215686274509803, "identity": 0.993, "score": 276.0, "evalue": 7.68e-94, "valid": true, "product": "Uncharacterized protein UU156", "uniref50_id": "UniRef50_Q9PQY8" }, "pscc": { "uniref50_id": "UniRef50_Q9PQY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQY8" ], "product": "Uncharacterized protein UU156" }, "genes": [], "id": "GPKLONFDEH_345", "locus": "GCA000006625_00312" }, { "type": "cds", "sequence": "contig_1", "start": 193934, "stop": 194092, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAGGAATCGATTTATCAACAACAATAACAGGAGTTGCTATTTTAAGAAATGAGCAGATAATTAAAACCTTTGCTGTTGCTTTTGATAACTTTAATGAAAAGAATTTATATAACAACGTTATAAGACTTATTAAAGAAATTAATTTAAATGTTTGA", "aa": "MIGIDLSTTITGVAILRNEQIIKTFAVAFDNFNEKNLYNNVIRLIKEINLNV", "aa_hexdigest": "807263e964dc031bea46a7f70fdd0958", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5908.820599999998, "isoelectric_point": 6.038597297668456 }, "id": "GPKLONFDEH_346", "locus": "GCA000006625_00313" }, { "type": "cds", "sequence": "contig_1", "start": 194291, "stop": 194599, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTAAAGATCGTAAATTAATTACTAAAAATTTAATGATTAAAACATTTAATATTAAAAGTAATTTAAACGAAGACGAATACGACGCCTTAGCGATTGCTTATTTTTATGATAGTATTAATTCAACGCTAGAACAAGAAGAGATAACTAAGGCTAAAAAAATAGTTAAAATGAATAAAGTTAAACAGCAGTTATCAATTAGTAAAAAGATTAATAAACTGCTTGAAAAGAAAAGCAAACTAAAAAAAGCAACTGCGATATCAAAAGTTGATGAACAAATAGAAGAATTAAAGAAACTAAATAAATAA", "aa": "MRKDRKLITKNLMIKTFNIKSNLNEDEYDALAIAYFYDSINSTLEQEEITKAKKIVKMNKVKQQLSISKKINKLLEKKSKLKKATAISKVDEQIEELKKLNK", "aa_hexdigest": "885a1e4c22f2692e5c7f86288ec64d49", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11946.993599999987, "isoelectric_point": 9.795149040222167 }, "id": "GPKLONFDEH_347", "locus": "GCA000006625_00314" }, { "type": "cds", "sequence": "contig_1", "start": 195503, "stop": 195733, "strand": "-", "frame": 3, "gene": null, "product": "putative protein UU158", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q9PQY6", "UniRef:UniRef90_Q9PQY6" ], "nt": "GTGTTCTCAGTGCTTGGATATTTAATAACAGCGTTATCAATTCCATTAAAAGTATTTGAATTAATGCTAATGCTAAGATTTAGATTAATGCTAGCCAAATTAATTAAGGATGGTATATTAGATCCAAAGGCGTTTATTGATGATATCAGACATAGTTATCTATCAAGACGTAAACAACGCAAATTAGAAGAAGAGATCGAATATTTAAAACGTATTAAAAGCGATTATTAG", "aa": "MFSVLGYLITALSIPLKVFELMLMLRFRLMLAKLIKDGILDPKAFIDDIRHSYLSRRKQRKLEEEIEYLKRIKSDY", "aa_hexdigest": "4a6c16cba6bcc977baee2aa0ed5f80b1", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 195503, "stop": 195946, "observations": { "mutation": [ 195763, 195877 ], "stop": [ 195763, 195877 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQY6", "query_cov": 0.9868421052631579, "subject_cov": 0.5102040816326531, "identity": 1.0, "score": 135.0, "evalue": 2.53e-39, "gene_start": 2, "gene_end": 76, "reference_start": 73, "reference_end": 147, "reference_sequence": "MTKQEFKAITKELFFFNKKRLMLWIAILIFVIAFAMIIVFVPFFNFNDKIKSLFDKLKHINWQDPTALFGLVFSVLGYLITALSIPLKVFELMLMLRFRLMLAKLIKDGILDPKAFIDDIRHSYLSRRKQRKLEEEIEYLKRIKSDY" }, "paralog": false, "identity": 0.9863945578231292, "score": 278.0, "evalue": 3.69e-98, "description": "Internal stop codon at 195763, 195877. Nonsense mutation around 195763, 195877.", "psc": { "uniref90_id": "UniRef90_Q9PQY6", "product": "Uncharacterized protein UU158", "uniref50_id": "UniRef50_Q9PQY6", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQY6", "UniRef:UniRef50_Q9PQY6", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQY6" ], "product": "Uncharacterized protein UU158" } }, "id": "GPKLONFDEH_348", "locus": "GCA000006625_00315" }, { "type": "cds", "sequence": "contig_1", "start": 195915, "stop": 196463, "strand": "-", "frame": 2, "gene": null, "product": "putative protein UU159", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010891692.1", "SO:0001217", "UniParc:UPI000013939C", "UniRef:UniRef100_Q9PQY5", "UniRef:UniRef50_Q9PQY5", "UniRef:UniRef90_Q9PQY5" ], "nt": "ATGATGAACAATAAAAATAAGTTTAAAAACAAAGAATGGAAGTTTTCTAAAAAACAAGTACAGCTGATTTATTTAACAAGTTCAATTATTTCAGGGTTATTTTTAGGATTAGCTTTATTATCTACTTATTTAATTGCTGGACTACCAAATGATAACGCATTTGTGTTATTTGTAAAAGAACAAAAGTTTTATTTTCCATTCTTTATGACTATTGGATTTATTAATTTAATTATTTCAATGTTAACACTTTTACCAACATTAAAAACATTATGGCGAACTGTTGCTAAAATGCACCAGTACGGGGATTTAAGTAAAGAAGAGTTCGAAGCCTTAGATATACTAGTTGAACAAATACGTAATCGTTATATTAGTGTTGAAAATATTAAAGCAGTTATAAGTTCTAACAATTACAAAACATTGGATGAAGAATTAAAAAAACTAGAACAACAAGAAAAGCAATTAAAAATACAAGAGCAAGAACAAAAAGTTAAGCGATTAGAACAGGAGATTATAAAAGATGACAAAACAAGAGTTCAAAGCGATTACTAA", "aa": "MMNNKNKFKNKEWKFSKKQVQLIYLTSSIISGLFLGLALLSTYLIAGLPNDNAFVLFVKEQKFYFPFFMTIGFINLIISMLTLLPTLKTLWRTVAKMHQYGDLSKEEFEALDILVEQIRNRYISVENIKAVISSNNYKTLDEELKKLEQQEKQLKIQEQEQKVKRLEQEIIKDDKTRVQSDY", "aa_hexdigest": "b46ef39c564c31a9393a51b31c03acce", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000013939C", "ncbi_nrp_id": "WP_010891692.1", "uniref100_id": "UniRef100_Q9PQY5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013939C", "RefSeq:WP_010891692.1", "UniRef:UniRef100_Q9PQY5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQY5", "uniref90_id": "UniRef90_Q9PQY5" }, "psc": { "uniref90_id": "UniRef90_Q9PQY5", "product": "Uncharacterized protein UU159", "uniref50_id": "UniRef50_Q9PQY5", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQY5" ], "product": "Uncharacterized protein UU159" }, "genes": [], "id": "GPKLONFDEH_349", "locus": "GCA000006625_00316" }, { "type": "cds", "sequence": "contig_1", "start": 197799, "stop": 198113, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGATGCTGTTGATAATAAAGTAAACGAAGTTAAAAAAGCAATTGATACTAAAGAAAATACATTCACTAAAAACTTAGAAAATAAAGTTAATGAAGCCAAACAAGCGGTTTTATCATCTTATGAGCAAGATAAAGCATCTGGTGTATTTAAAGGTGAAAAAGGCGAACCTGGAATACAAGGTCCAATTGGTCCAAAAGGTGAAACTGGTCCAAAGGGTGAAAAAGGTGATAGTGCTAATGTTGATGCTTTAACATCTAATTTAGAAAGTTTTAAAAACACTGCACTAAATAGGAATGTTAGGTTTCCGTGA", "aa": "MQDAVDNKVNEVKKAIDTKENTFTKNLENKVNEAKQAVLSSYEQDKASGVFKGEKGEPGIQGPIGPKGETGPKGEKGDSANVDALTSNLESFKNTALNRNVRFP", "aa_hexdigest": "28a306acd817f42733329230b5c4a5ca", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11209.281299999988, "isoelectric_point": 6.307332038879395 }, "id": "GPKLONFDEH_350", "locus": "GCA000006625_00317" }, { "type": "cds", "sequence": "contig_1", "start": 198162, "stop": 198320, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACTGGGATGCTATTTATGACTATAAAGCTGGTGAGTTAGTTTATGACTTAGATGAAAAAACTAACACAATTAAAGTTTATAAGGCACTAGTTAACAATGGTGTAAGTACGCATAATATCGTAGATCCAAGTAAGCAAATTAACGAAAATGTATGA", "aa": "MYWDAIYDYKAGELVYDLDEKTNTIKVYKALVNNGVSTHNIVDPSKQINENV", "aa_hexdigest": "1f0fc25ef2e72e6adc2074068b15aa4e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5993.618800000003, "isoelectric_point": 4.701573753356932 }, "id": "GPKLONFDEH_351", "locus": "GCA000006625_00318" }, { "type": "cds", "sequence": "contig_1", "start": 198942, "stop": 199139, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATAGATTATTACTTAGTGTAGAAGAATATTTAAGTTTGTTTCCACTAATTGTACGAAACGAAAATTTAGAAGATAACCAATTACAATTAGAACGTAAAAAACAAGCGTTAACTACGTTTATTTTGCGTGCTCAATCAATGATTGATGATTGTGTAGCATCAAATGGTGGTGCTGGTTTTATTTATAGTTGA", "aa": "MNNRLLLSVEEYLSLFPLIVRNENLEDNQLQLERKKQALTTFILRAQSMIDDCVASNGGAGFIYS", "aa_hexdigest": "9a8518864bc66bf7b9a3ff3126785bb8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7405.402000000002, "isoelectric_point": 4.812637138366699 }, "id": "GPKLONFDEH_352", "locus": "GCA000006625_00319" }, { "type": "cds", "sequence": "contig_1", "start": 199394, "stop": 199921, "strand": "-", "frame": 3, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA87CUW9", "UniRef:UniRef90_A0AA87CUW9" ], "nt": "ATGGGTATACTAATTCTAATACTACGCTTTGTTGGTCGCTTGTTGTTATCTAAAATTAAGAGTTTAATATCTACTTTGATTAACTTATTTTCACGTATGTTAAAGATGTTTAAATTTGATTTTTTTAAAATGTTTAAAAATAAAACTGGTGCTTTTATGAAAAAAGGTAAAACTGTTTTTCATGACTTTAATTTAAAACCAGATGACATATTAAAAAAGTTTTTTGATAATTCAAGTTTAGAAAAGATTTTTAACTCAAAAGAATTAAAAATTATTCAAGATAACAAAGATTTAGCTAAAAAACTAAAAGATGCTATTAATGAAGCGATTAAAGATATTGCTAAACAAACTAGTAAATACACGACAAAACAAAAAGATAGCATTATCAAACATTTAACAGATACATTAGATAACGCATCTAAAACTGAAAAAAAGAATTTTGAAAAAGTGCGTAAAGAAAATCAGTTTAAAGATGTTGATAATGTTGACATTGATTTTAATAAACATTGGATAGATTATGGCACATGA", "aa": "MGILILILRFVGRLLLSKIKSLISTLINLFSRMLKMFKFDFFKMFKNKTGAFMKKGKTVFHDFNLKPDDILKKFFDNSSLEKIFNSKELKIIQDNKDLAKKLKDAINEAIKDIAKQTSKYTTKQKDSIIKHLTDTLDNASKTEKKNFEKVRKENQFKDVDNVDIDFNKHWIDYGT", "aa_hexdigest": "329756719488c5be7868286c12bf831f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 199139, "stop": 199921, "observations": { "mutation": [ 199180, 199243, 199306, 199396 ], "stop": [ 199180, 199243, 199306, 199396 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AA87CUW9", "query_cov": 1.0, "subject_cov": 0.6730769230769231, "identity": 1.0, "score": 317.0, "evalue": 6.05e-108, "gene_start": 1, "gene_end": 175, "reference_start": 1, "reference_end": 175, "reference_sequence": "MGILILILRFVGRLLLSKIKSLISTLINLFSRMLKMFKFDFFKMFKNKTGAFMKKGKTVFHDFNLKPDDILKKFFDNSSLEKIFNSKELKIIQDNKDLAKKLKDAINEAIKDIAKQTSKYTTKQKDSIIKHLTDTLDNASKTEKKNFEKVRKENQFKDVDNVDIDFNKHWIDYGTWYASDGVGKIGYLTLKLMKPSKRNPLARYTWRNIPKRIATMLKFAPTGTTFWNIFYHANRKNLIHLQKESIYWFDASRKYFEGVK" }, "paralog": false, "identity": 0.9846153846153847, "score": 504.0, "evalue": 5.81e-184, "description": "Internal stop codon at 199180, 199243, 199306, 199396. Nonsense mutation around 199180, 199243, 199306, 199396.", "psc": { "uniref90_id": "UniRef90_A0AA87CUW9", "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0AA87CUW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AA87CUW9", "UniRef:UniRef50_A0AA87CUW9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AA87CUW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA87CUW9" ], "product": "ATP-binding protein" } }, "id": "GPKLONFDEH_353", "locus": "GCA000006625_00320" }, { "type": "cds", "sequence": "contig_1", "start": 200001, "stop": 200186, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAGTGCGGTCTTTCCACAATTTGCACATTTAAATTTTGTGATTTTAAATGATAAACCAACAAAAGATGAAATCAATAAAGCACGCTCAAATCTTTATTCACAATTTCCCTCACTATATGCTGAATTTAAAACACCAATCAGTGAAGATTCTAAAATCACTGAAGTAAATAAAGCAAATTGA", "aa": "MNSAVFPQFAHLNFVILNDKPTKDEINKARSNLYSQFPSLYAEFKTPISEDSKITEVNKAN", "aa_hexdigest": "896ba3a2be786af2a5a5f76e66657399", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6962.759900000001, "isoelectric_point": 6.538553047180176 }, "id": "GPKLONFDEH_354", "locus": "GCA000006625_00321" }, { "type": "cds", "sequence": "contig_1", "start": 200190, "stop": 200420, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGATTATTTAGGAATGCCAATATACTCATCTGCATATTTAGGCGAAGATTATTTAAAATCAACAGATCAAAAATACACAAGTAATTTAATTCGTGATTACAAGTTTAGCAAACTAAGCGGTATTATTACAATGCCTGAGACTACAATTTGTTTAAAACAAGAATTAATGTTAACAACATTTCAAAGTCCAACAAGCAAATCACACCAGTTAATTCATAACTCATGA", "aa": "MGDYLGMPIYSSAYLGEDYLKSTDQKYTSNLIRDYKFSKLSGIITMPETTICLKQELMLTTFQSPTSKSHQLIHNS", "aa_hexdigest": "10d0644b2b919bdc92c7be3fb9ce764b", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8674.837599999995, "isoelectric_point": 6.701339912414551 }, "id": "GPKLONFDEH_355", "locus": "GCA000006625_00322" }, { "type": "cds", "sequence": "contig_1", "start": 200799, "stop": 201041, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGAAAATCAATTTAACGCTGAATTTCTAGGAAGTGCAACAATGACACCAACTAAAATTATGGATGATATGCGAACTGCTATTTATGAAGTTGGCGTAATCGGACAAGTTGAAGAGTACCGTGATGACTTTACACAAAAGCAAGTACCAGCAATTGATAGATTAACATTACATTTAAGTAATGACTATCAAAAAGAAGTAAGTATTAGTGTATTTGATAAAAAAACAGGTGCTCAATGA", "aa": "MLENQFNAEFLGSATMTPTKIMDDMRTAIYEVGVIGQVEEYRDDFTQKQVPAIDRLTLHLSNDYQKEVSISVFDKKTGAQ", "aa_hexdigest": "be554d989176c2f6661bc823a6400247", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9119.174399999998, "isoelectric_point": 4.610062980651855 }, "id": "GPKLONFDEH_356", "locus": "GCA000006625_00323" }, { "type": "cds", "sequence": "contig_1", "start": 201180, "stop": 201506, "strand": "-", "frame": 2, "gene": null, "product": "putative protein UU165", "db_xrefs": [ "RefSeq:WP_010891696.1", "SO:0001217", "UniParc:UPI00001393B0", "UniRef:UniRef100_Q9PQX9", "UniRef:UniRef50_Q9PQX9", "UniRef:UniRef90_Q9PQX9" ], "nt": "ATGGTTGATGAACTTGAAAAAAATCAAGTTCAACCACAAGAAACTGAAGAAAATAAAGAAAATGCTTTATATGATGAAATTGACAAACGTATTCAAAAACAATTTGAAAATTATCTAAAAACAAATGAACAATTAGATAATGCTAATAAATTAGTTGAAGCTAAGTTAAAAGAAATCAAAGACTTAGAAAATACGTTTAAGCAACGAGAACAAGAATTATTAAACAAAATCGCAACGCTTGAACAAAATCAACAGTTTGTTAATATTCATCAAGAAAAACCAGTCGTAGAAGATGCTAATAGCCACTTTTTAAAATTTTTTAACTAA", "aa": "MVDELEKNQVQPQETEENKENALYDEIDKRIQKQFENYLKTNEQLDNANKLVEAKLKEIKDLENTFKQREQELLNKIATLEQNQQFVNIHQEKPVVEDANSHFLKFFN", "aa_hexdigest": "2b33d407ffec11b18efbcd5805043389", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00001393B0", "ncbi_nrp_id": "WP_010891696.1", "uniref100_id": "UniRef100_Q9PQX9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393B0", "RefSeq:WP_010891696.1", "UniRef:UniRef100_Q9PQX9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQX9", "uniref90_id": "UniRef90_Q9PQX9" }, "psc": { "uniref90_id": "UniRef90_Q9PQX9", "product": "Uncharacterized protein UU165", "uniref50_id": "UniRef50_Q9PQX9" }, "pscc": { "uniref50_id": "UniRef50_Q9PQX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX9" ], "product": "Uncharacterized protein UU165" }, "genes": [], "id": "GPKLONFDEH_357", "locus": "GCA000006625_00324" }, { "type": "cds", "sequence": "contig_1", "start": 201509, "stop": 201712, "strand": "-", "frame": 3, "gene": null, "product": "putative protein UU165-2", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q9PQX8", "UniRef:UniRef90_Q9PQX8" ], "nt": "GTGAAATTTTACTCTTTTAGTTTAGTTGATAAACCATTCGCAAGAGGAGTACCAAACAGTATTGATGTATTCTCTCGAGCCATTATCGGTGTAGCGTTTGGCTTAGGATTTGTAGGAACTATGCTAATTGATTATTTCATAATCTCAAAAGTTGCGTATATTGTGAAACAAAAAATAAAACAATCTAAGAAAGTAGGTATGTAA", "aa": "MKFYSFSLVDKPFARGVPNSIDVFSRAIIGVAFGLGFVGTMLIDYFIISKVAYIVKQKIKQSKKVGM", "aa_hexdigest": "4f4fe8ec9d1478a8e6124e6d9604a282", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 201509, "stop": 201877, "observations": { "mutation": [ 201772, 201832, 201838 ], "stop": [ 201772, 201832, 201838 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQX8", "query_cov": 1.0, "subject_cov": 0.5491803278688525, "identity": 0.985, "score": 127.0, "evalue": 9.07e-37, "gene_start": 1, "gene_end": 67, "reference_start": 56, "reference_end": 122, "reference_sequence": "MNKIIKFHNERIKWLWILTAILAISFFVICFNNVKWIYTENTAKYELLTSSLEKIVKFYSFSLVDKPFARGVPNSIDVFSRAIIGVAFGLGFVGTMLIDYFIISKVAYIVKQKIKQSKKVGM" }, "paralog": false, "identity": 0.9754098360655737, "score": 226.0, "evalue": 2.63e-78, "description": "Internal stop codon at 201772, 201832, 201838. Nonsense mutation around 201772, 201832, 201838.", "psc": { "uniref90_id": "UniRef90_Q9PQX8", "product": "Uncharacterized protein UU165.2", "uniref50_id": "UniRef50_Q9PQX8", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQX8", "UniRef:UniRef50_Q9PQX8", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQX8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX8" ], "product": "Uncharacterized protein UU165.2" } }, "id": "GPKLONFDEH_358", "locus": "GCA000006625_00325" }, { "type": "cds", "sequence": "contig_1", "start": 202718, "stop": 202918, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCGATAATTCATTATTAAGTGCTACGCAATATAGAAAACCACGCTTTTATTTTTATGAACCAGACAGTTCTAAATTAGCAAATCTATTTGGTTATGGTTCATATACTAATAAACTAAATTATTTATTTGGTGGAAGTAATTTAAGTGATGTAATTGTTGCTAATAAAGTTATTGCTTATAGTTTTGTGTTTAAATGA", "aa": "MGDNSLLSATQYRKPRFYFYEPDSSKLANLFGYGSYTNKLNYLFGGSNLSDVIVANKVIAYSFVFK", "aa_hexdigest": "fe912855ba9d98c780080c0fa217dd43", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7492.387500000002, "isoelectric_point": 9.301321220397949 }, "id": "GPKLONFDEH_359", "locus": "GCA000006625_00326" }, { "type": "cds", "sequence": "contig_1", "start": 203084, "stop": 203221, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTAAGTGTGATATTAAACTAACAGCTTTTAGTGGCTTTTATTCACCAGATGCACGATTAAAAGACGAATTTAAAAACGTGTACGAAGCGATTTTTGAAAGCAATGGAAAATATACTGATGATATGAGTCAATGA", "aa": "MGKCDIKLTAFSGFYSPDARLKDEFKNVYEAIFESNGKYTDDMSQ", "aa_hexdigest": "d2adf4dfcdc2523b15ed8ce3971814cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5161.706800000001, "isoelectric_point": 4.716124534606934 }, "id": "GPKLONFDEH_360", "locus": "GCA000006625_00327" }, { "type": "cds", "sequence": "contig_1", "start": 203505, "stop": 203672, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTATGCCAAGTGGACTAAATGTTTTAGCTGGTTTGTTAGTGAAAATATCTAAATTAATTAAATCCGCTTTTAGTTTATTTGATACTTCTAATTCAAGCGGTAAACATGGTGCAATAAAATTAAAACCACGTGTATTTAATCAAATGTTTGACATGTTATAA", "aa": "MAGMPSGLNVLAGLLVKISKLIKSAFSLFDTSNSSGKHGAIKLKPRVFNQMFDML", "aa_hexdigest": "65c6e366d9fd6cb7f2265eb6a9823c4f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5911.0352, "isoelectric_point": 10.294907951354979 }, "id": "GPKLONFDEH_361", "locus": "GCA000006625_00328" }, { "type": "cds", "sequence": "contig_1", "start": 203696, "stop": 204031, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTACAGTGTTTGAAAGTATTGGACGTAATGTTTCACAAGGATTTAATTTAAATAATTTAGGTTTATGGTCTTTTAATATGGGATTTGCTAAAACCAAAGCTGAACAAATAAAAGAAGCAACTAAAATTGGATTAAACAACATTACAATGCCACCTGGTACTAATTTGCATACGTTTTTAAATCACAATGCATTTATGTATGATTTGATTAAGTATTTACCATATTCATACAAGGAAACAATCCGTCAAAATCTTGGTGAATTAATAGATAATACACCACTTAGTTTAGGTAAAGTTAGACGTGGTATTGTAGATATTTTATATCCTGGTTGA", "aa": "MSTVFESIGRNVSQGFNLNNLGLWSFNMGFAKTKAEQIKEATKIGLNNITMPPGTNLHTFLNHNAFMYDLIKYLPYSYKETIRQNLGELIDNTPLSLGKVRRGIVDILYPG", "aa_hexdigest": "25da853a8dfb0a04656e18f23f13419d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12526.283299999985, "isoelectric_point": 9.30054759979248 }, "id": "GPKLONFDEH_362", "locus": "GCA000006625_00329" }, { "type": "cds", "sequence": "contig_1", "start": 204236, "stop": 204499, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATAACAGGACGAACTTATAATAATGACACACGAGAGTTAAGTGCTAATAAGCGATTATTACTATATAAAGCTAAAATACCAGTCGCATCTAAATTCTTTAATGTATCAGCACCAACAAGTAATTATGTATATTTATACAATGCGTTTTTTGAAATTGAAAAGATTTATAAAGACCAGTATGAAAAACAAAAAGATTTTACATTTGACCATAACGCTAATATTAAATTAAATGGTGGATTATTATTAAATCATAAATGA", "aa": "MLITGRTYNNDTRELSANKRLLLYKAKIPVASKFFNVSAPTSNYVYLYNAFFEIEKIYKDQYEKQKDFTFDHNANIKLNGGLLLNHK", "aa_hexdigest": "88bb9622876555b110d0f092ee431869", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10213.55259999999, "isoelectric_point": 9.487376976013184 }, "id": "GPKLONFDEH_363", "locus": "GCA000006625_00330" }, { "type": "cds", "sequence": "contig_1", "start": 204605, "stop": 204760, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATGAAGTCATTCCTTTTTATTCAACAACATCAAAACGTTATGAAGGAGCACAAATTACACTAACAAACGTTAACGATTTACTAACATCATCTGGTAATGATATATTAGCAACAATTCAACCATGTCAACCTGGAGATGGTTATTTATGA", "aa": "MVNEVIPFYSTTSKRYEGAQITLTNVNDLLTSSGNDILATIQPCQPGDGYL", "aa_hexdigest": "692f37304af4eb9ec357c5ad3734e918", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5550.144400000001, "isoelectric_point": 4.104764366149902 }, "id": "GPKLONFDEH_364", "locus": "GCA000006625_00331" }, { "type": "cds", "sequence": "contig_1", "start": 205175, "stop": 205369, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAATATTTATTTAGATGAACTTGAAGGTTATAGTCAAAAAAATATTGAAGACAGCATTGATAGTTTAATTGAACCAAACCAAAAACCAATTCATTTATGTCCATTTAGAATACTAAAAGGTTTTATTAAAATTACAAACACCGGTGAACAATCATTATTAGTAAATGACAAAGATTTTAGCACTGTTTGA", "aa": "MSNIYLDELEGYSQKNIEDSIDSLIEPNQKPIHLCPFRILKGFIKITNTGEQSLLVNDKDFSTV", "aa_hexdigest": "3d41176a4348a76e42ac8b93bfd1a100", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7314.217099999999, "isoelectric_point": 4.642290687561036 }, "id": "GPKLONFDEH_365", "locus": "GCA000006625_00332" }, { "type": "cds", "sequence": "contig_1", "start": 205362, "stop": 205520, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAAGTCATTAGTTTATATGGTGCTAATTTAATGCCACTTAATTTAGCTGTACAAAAATTATATCCAGAACTTACACCTACCGAACAAGATGAACTAGTAAAAGAATTAGAACATAAACAAAAACTAGAAAATGGAGAGTTTGAAAATGAGTAA", "aa": "MEKVISLYGANLMPLNLAVQKLYPELTPTEQDELVKELEHKQKLENGEFENE", "aa_hexdigest": "5cacfd0a71fd8be811263d7a434443f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6044.811799999998, "isoelectric_point": 4.585906410217285 }, "id": "GPKLONFDEH_366", "locus": "GCA000006625_00333" }, { "type": "cds", "sequence": "contig_1", "start": 205799, "stop": 205942, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTCAGATCTGATATTAAACACAAACACACCAGATAAAAACGCATTTGAAATTATGCAATCTACTTTTAACGAAAGTATTCACTTACAAGCCATTACTGAAAAATTAAATTTAATCTTTAAAGGTTGTGGTTATGTATGA", "aa": "MSSDLILNTNTPDKNAFEIMQSTFNESIHLQAITEKLNLIFKGCGYV", "aa_hexdigest": "66c22759490850e249fb01dd9407da7d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5290.994599999998, "isoelectric_point": 4.895849418640137 }, "id": "GPKLONFDEH_367", "locus": "GCA000006625_00334" }, { "type": "cds", "sequence": "contig_1", "start": 206096, "stop": 206647, "strand": "-", "frame": 3, "gene": null, "product": "putative protein UU168", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX5", "UniRef:UniRef90_Q9PQX5" ], "nt": "ATGATTGCACGAACTGCACAAGAAAGTACTGTTAATGATGTTATCTTAAAAACAAAAGATAATGTACCTTTGGAGTGGATGGAATTTATTGATAATATTAATGAAAACAACGAAATACTAAAACTAATTAAAGGTGATTGTCGTATTAAATCACTATATGGTATTAACTATGTTGGTTTTGAAATATATAACAATGATATTTTAATTTTTAATGCAGATATTAATAACGCTATGAACAAATCCATTAGGATTAATCAACAACAAGAGTATAGCAGTACTATTTATCGTAAAATTGCGTTAATGAAAGATAGTGAATATTTAAGTTTAAAAGAACTACACACACCATACGAAATTAAACGATTAATCTATAACTCAACTGGTGTATATAAAATTATTTTTAATGAATTTGAAGTTTCTACACCAATGTATTTTAAAGATATTTTTAAAAACAAAAACGTCGTACACAACTACGGCGTATTGTGCACAAAAGAGTTTTTAAATCGTTCATTTATTGACTTTGAAAATAAAATTAATGAGAAATTACCAGATTGA", "aa": "MIARTAQESTVNDVILKTKDNVPLEWMEFIDNINENNEILKLIKGDCRIKSLYGINYVGFEIYNNDILIFNADINNAMNKSIRINQQQEYSSTIYRKIALMKDSEYLSLKELHTPYEIKRLIYNSTGVYKIIFNEFEVSTPMYFKDIFKNKNVVHNYGVLCTKEFLNRSFIDFENKINEKLPD", "aa_hexdigest": "018c1287931e77000a1bf03efd72938b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 205793, "stop": 206725, "observations": { "mutation": [ 205801, 205963, 206098, 206689 ], "stop": [ 205801, 205963, 206098, 206689 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQX5", "query_cov": 1.0, "subject_cov": 0.543026706231454, "identity": 1.0, "score": 367.0, "evalue": 1.93e-126, "gene_start": 1, "gene_end": 183, "reference_start": 27, "reference_end": 209, "reference_sequence": "MALKTNTIIKSKWNPSNFDINIDIKEMIARTAQESTVNDVILKTKDNVPLEWMEFIDNINENNEILKLIKGDCRIKSLYGINYVGFEIYNNDILIFNADINNAMNKSIRINQQQEYSSTIYRKIALMKDSEYLSLKELHTPYEIKRLIYNSTGVYKIIFNEFEVSTPMYFKDIFKNKNVVHNYGVLCTKEFLNRSFIDFENKINEKLPDWYPVNDLINDTNALLNFIKKERELNKTRIVGSIINHNDAQTYRNQWIDEIQYMSSDLILNTNTPDKNAFEIMQSTFNESIHLQAITEKLNLIFKGCGYVWNMGDSLSYQNSETATNLNKATFETQKWK" }, "paralog": false, "identity": 0.9870967741935484, "score": 607.0, "evalue": 1.48e-223, "description": "Internal stop codon at 205801, 205963, 206098, 206689. Nonsense mutation around 205801, 205963, 206098, 206689.", "psc": { "uniref90_id": "UniRef90_Q9PQX5", "product": "Uncharacterized protein UU168", "uniref50_id": "UniRef50_Q9PQX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQX5", "UniRef:UniRef50_Q9PQX5" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX5" ], "product": "Uncharacterized protein UU168" } }, "id": "GPKLONFDEH_368", "locus": "GCA000006625_00335" }, { "type": "cds", "sequence": "contig_1", "start": 206715, "stop": 206954, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17289.8" ], "nt": "ATGCTTGCAAGTGGTAAATTGCGATTTAGTAAAAATGCGTGTCCAAAACTATATAAGGACTTAAAAAATTGCTACGTTGTTTTACCAAAAACCATTAAAGAAGATACTAAAAAAGAGCGTAGTCATGAGCATACGCATACGATTAATGCGTTGGAGTATTTAATGTGTGCGTTTCATTTATATTTTTATGCACAAGCACCTGCAATTAATGAAAAAGGAGCTATAAGATATGGCACTTAA", "aa": "MLASGKLRFSKNACPKLYKDLKNCYVVLPKTIKEDTKKERSHEHTHTINALEYLMCAFHLYFYAQAPAINEKGAIRYGT", "aa_hexdigest": "85206bc7215e6b4f14872eee806dbbd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17289.8", "name": "Terminase_6C", "length": 58, "aa_cov": 0.7215189873417721, "hmm_cov": 0.896551724137931, "evalue": 1.2598059174631585e-10, "score": 37.10947799682617, "start": 1, "stop": 57 } ], "seq_stats": { "molecular_weight": 9123.545599999992, "isoelectric_point": 9.349865913391113 }, "id": "GPKLONFDEH_369", "locus": "GCA000006625_00336" }, { "type": "cds", "sequence": "contig_1", "start": 206979, "stop": 207419, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17289.8" ], "nt": "TTGGGATTAAGTGGGAGTGTTGAAGGTGCTATTTATGCACGAAGTTTAAGCGTAATGCATGAACCATATGGCGAATATGATAATTATGCAATGCAGTTTTTTGGTGGTATGGACTGGGGAGATGGAACAAGTAGTTCATCATCAGATACTGCGTGCTTATTTGGTTCAATTTCAAAAGAAAATGGAATTGAGATTTTAGATGAAATGGTTTATAACAACAATGTTAATCCACTAACAAGCGAGCAACAAATTGAGTTAGTTGTGCGTTGGTTTATTAAAAAACAACAAGAAGTAAACGTTAAGATTGTAATTTATATTGATAATGCTCATTTGCGTTATTTTTATGAAACATTTAACGCTACATTTAAACGCATTGCGTTTCAATATAATATGTATGGTTCTGAACTTGTTTTTGCACCTGCTATTAAACACGCTATTTGA", "aa": "MGLSGSVEGAIYARSLSVMHEPYGEYDNYAMQFFGGMDWGDGTSSSSSDTACLFGSISKENGIEILDEMVYNNNVNPLTSEQQIELVVRWFIKKQQEVNVKIVIYIDNAHLRYFYETFNATFKRIAFQYNMYGSELVFAPAIKHAI", "aa_hexdigest": "c34169a0dc6c762971c07a5c2aa9cc11", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17289.8", "name": "Terminase_6C", "length": 101, "aa_cov": 0.6917808219178082, "hmm_cov": 0.8910891089108911, "evalue": 2.016406433248874e-15, "score": 52.68674087524414, "start": 35, "stop": 135 } ], "seq_stats": { "molecular_weight": 16584.49959999999, "isoelectric_point": 4.8213903427124025 }, "id": "GPKLONFDEH_370", "locus": "GCA000006625_00337" }, { "type": "cds", "sequence": "contig_1", "start": 207843, "stop": 208160, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAATAAAACTAGATTTTATAGAATGTTTTGGTTTCTATAGTGATTTAGTTGAGTTTGTAGCCAAAAAGTTGCCAATACCATTTAATGAAATTAATTTATTAGGTGGACGAAGTAGTGGTAAGACTTTTGCGACATTAGATTTTTTGTCATCACTTGTTGCGATTAAACAGCAAAACGCAATATATGTTTTTCGTCAGAATATATCTGATGCGAAAAAAATTTTTAGCGACTATTTAAATTTTTTAGACCAAAAGCAATTATTAAAATTTGCTAAGATTAACCATTCAGAACGTACTGTTATTATTAATGGTTGA", "aa": "MRIKLDFIECFGFYSDLVEFVAKKLPIPFNEINLLGGRSSGKTFATLDFLSSLVAIKQQNAIYVFRQNISDAKKIFSDYLNFLDQKQLLKFAKINHSERTVIING", "aa_hexdigest": "a8602bfaacec69585801e7d8a3b94430", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12054.899499999983, "isoelectric_point": 9.335231590270997 }, "id": "GPKLONFDEH_371", "locus": "GCA000006625_00338" }, { "type": "cds", "sequence": "contig_1", "start": 208517, "stop": 208735, "strand": "-", "frame": 3, "gene": null, "product": "putative protein UU170", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX3", "UniRef:UniRef90_Q9PQX3" ], "nt": "ATGGCTTTAAATGAGTTGCTGGGACGAAACGCATCGTATGATAAAGAAATAAACAACCAAGGATTGCGTAAGTTTAGAACGCAAGAATATTTTAAACGTCTAGATTTTGAGTTAGCAAATGAGCAAAAAAGTACAGATATTGAAGATACAACAATAACGTTTGAAAAAGTTGATGATAATAACAAATTAACCATTAAAAAACTTATAAATGTGATATAA", "aa": "MALNELLGRNASYDKEINNQGLRKFRTQEYFKRLDFELANEQKSTDIEDTTITFEKVDDNNKLTIKKLINVI", "aa_hexdigest": "89428e69c59283ca90eb0f2cd4e57bed", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 208517, "stop": 208960, "observations": { "mutation": [ 208816 ], "stop": [ 208816 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQX3", "query_cov": 1.0, "subject_cov": 0.4897959183673469, "identity": 1.0, "score": 136.0, "evalue": 7.48e-40, "gene_start": 1, "gene_end": 72, "reference_start": 76, "reference_end": 147, "reference_sequence": "MMKRNELKQLQELQLKINDFYTLVMTTKNLDLCNLSALLEFLGINWGVWVNLSTQRDEFNKKRYEYITKANYGLRMALNELLGRNASYDKEINNQGLRKFRTQEYFKRLDFELANEQKSTDIEDTTITFEKVDDNNKLTIKKLINVI" }, "paralog": false, "identity": 0.9931972789115646, "score": 290.0, "evalue": 8.72e-103, "description": "Internal stop codon at 208816. Nonsense mutation around 208816.", "psc": { "uniref90_id": "UniRef90_Q9PQX3", "product": "Uncharacterized protein UU170", "uniref50_id": "UniRef50_Q9PQX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQX3", "UniRef:UniRef50_Q9PQX3" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX3" ], "product": "Uncharacterized protein UU170" } }, "id": "GPKLONFDEH_372", "locus": "GCA000006625_00339" }, { "type": "cds", "sequence": "contig_1", "start": 209440, "stop": 210003, "strand": "-", "frame": 1, "gene": null, "product": "putative protein UU171", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX2", "UniRef:UniRef90_Q9PQX2" ], "nt": "GTGATTGCAAATAAAATAACAATTAATAAAATTCAAAACGGCACTGCTACTATTGAAGTTACATTTAGTAAATTTAAATTAGCAGATGCTAATAAAAAAGATTTTGTTTTAGAAGTTAAAGATAGTGCTAATGATGACGCTTCTGCTATTAGTGCTACTGAATTAGTATTTGATGCAAATAAAAAAATACTTACAGGTAAATTAAATGGCTTAGCATCTAATATTAACTATAAAATATCAAAATTTACTTTAAATAATAATGAAATTAAATTTGATTTTAAACAAGAAGACCAACAAGAATTATTAAACCAATATATTCAACAAGCAGAATTAAATACAATAATTGATAAAGCTAATAAAAAAATTAACATTAAATTACAAAATTTTAGTATATTAAATTCTTTTCAAGATAATCAACCTGTATTAACATTTGATATTGAAATTAATAAAAAAGACGGAATAACACAAGTTGTCCATAAATCGTTAACAAAGAATCAATTATTAAATCCTAATGGTTTAGAAATTGATTTAAAAGATAAAATGAAAAATAATATTGATTATTGA", "aa": "MIANKITINKIQNGTATIEVTFSKFKLADANKKDFVLEVKDSANDDASAISATELVFDANKKILTGKLNGLASNINYKISKFTLNNNEIKFDFKQEDQQELLNQYIQQAELNTIIDKANKKINIKLQNFSILNSFQDNQPVLTFDIEINKKDGITQVVHKSLTKNQLLNPNGLEIDLKDKMKNNIDY", "aa_hexdigest": "402cf638ff17d0825061bcab9ffb2668", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PQX2", "query_cov": 1.0, "subject_cov": 0.8947368421052632, "identity": 0.995, "score": 348.0, "evalue": 1.09e-120, "valid": true, "product": "Uncharacterized protein UU171", "uniref50_id": "UniRef50_Q9PQX2" }, "pscc": { "uniref50_id": "UniRef50_Q9PQX2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX2" ], "product": "Uncharacterized protein UU171" }, "genes": [], "id": "GPKLONFDEH_373", "locus": "GCA000006625_00340" }, { "type": "cds", "sequence": "contig_1", "start": 210411, "stop": 211115, "strand": "+", "frame": 3, "gene": null, "product": "MBA N-terminal paralog", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAX8", "UniRef:UniRef90_D2EDJ8" ], "nt": "ATGTCATTAAGTGCGATTGCAATTAGTACAAGTATAGTTGCGTTCGCAGCTTCTTGTTCAAATACAAATATTAAATCTAAGTTAAGCAAGCAATTAGCACAAACAAAAGATCAAAAAAACCATTTTGCTGTTTATGAAATTGAAAATTATACTAAACTAACAGATGAAGAAAAGAAGTCATTAGAAAATCTTGAGTTTGGTGTTTCTATTTTGGATAAAGATGGTAAAAATCCAATAAATATTAAAGTTAAAGGAGTTCAAAAAGAAGGTAAGATTTACGTTAAATTGCCACGCAAACCAGAAGTAAATGAAAAATTACTTGTTGTACCAACTAAAAATATTTTAAAAACAGAAGCATTAGTTTTAAATAATGATAATTTAAATTATCAAACAGTAACTTTTGAACAAGTTCCTGATCAAAAATTAGAACCAAAAGAAGAAAAACAAACTAAAGCTGTTGATTATGAAAAAGCAATTAAAGATGAAGATTATGTAAAACAAATTACATTATCTAATTTAGAAAAATATATTAGTAATGAGTATCAATATTTAAATCCAAATAATAAAACACTAGATTATAAAAATCATAGTTTTGTTCAAAAGTTTTTAGACGAAATTGATCTTTTAGCACAAAAATTAGAAAGCAACGACCTTAATTTAGAAGATAAACAAATTTATGAAAAAATTATTTTAGGATTACGTTGA", "aa": "MSLSAIAISTSIVAFAASCSNTNIKSKLSKQLAQTKDQKNHFAVYEIENYTKLTDEEKKSLENLEFGVSILDKDGKNPINIKVKGVQKEGKIYVKLPRKPEVNEKLLVVPTKNILKTEALVLNNDNLNYQTVTFEQVPDQKLEPKEEKQTKAVDYEKAIKDEDYVKQITLSNLEKYISNEYQYLNPNNKTLDYKNHSFVQKFLDEIDLLAQKLESNDLNLEDKQIYEKIILGLR", "aa_hexdigest": "ed685e27652e16e1d5d634009fadd546", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 210378, "stop": 211418, "observations": { "mutation": [ 210405, 211113, 211398 ], "stop": [ 210405, 211113, 211398 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D2EDJ8", "query_cov": 1.0, "subject_cov": 0.6206896551724138, "identity": 0.996, "score": 433.0, "evalue": 5.93e-151, "gene_start": 1, "gene_end": 234, "reference_start": 12, "reference_end": 245, "reference_sequence": "MKLLKNKKFWAMSLSAIAISTSIVAFAASCSNTNIKSKLSKQLAQTKDQKNHFAVYEIENYTKLTDEEKKSLENLEFGVSILDKDGKNPINIKVKGVQKEGKIYVKLPRKPEVNEKLLVVPTKNILKTEALVLNNDNLNYQTATFEQVPDQKLEPKEEKQTKAVDYEKAIKDEDYVKQITLSNLEKYISNEYQYLNPNNKTLDYKNHSFVQKFLDEIDLLAQKLESNDLNLEDKQIYEKIILGLRWLLAEDNNETKNKALELFKDYFTTIMKATKVNDKVHYDPSFINEFYGSISNLFEEHDLEPSVEIESNKIRIVKEDKDVIKDKTVTATFNLYNGFDWSTLLKDVVFKEYKKDSNGKPVIDEKTKMPIETDKIV" }, "paralog": false, "identity": 0.9884393063583815, "score": 666.0, "evalue": 2.42e-245, "description": "Internal stop codon at 210405, 211113, 211398. Nonsense mutation around 210405, 211113, 211398.", "psc": { "uniref90_id": "UniRef90_D2EDJ8", "product": "MBA N-terminal paralog", "uniref50_id": "UniRef50_B5ZAX8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D2EDJ8", "UniRef:UniRef50_B5ZAX8" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZAX8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZAX8" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_374", "locus": "GCA000006625_00341" }, { "type": "cds", "sequence": "contig_1", "start": 211116, "stop": 211400, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAGCAGAAGATAATAATGAAACTAAAAATAAAGCTTTAGAATTATTTAAAGATTACTTTACAACAATCATGAAAGCAACAAAAGTAAATGATAAAGTTCATTATGATCCAAGTTTTATTAATGAATTTTATGGTTCAATAAGCAATTTGTTTGAAGAGCATGATTTAGAGCCATCAGTGGAAATTGAATCTAATAAAATCCGTATTGTTAAAGAAGATAAAGATGTAATAAAAGACAAAACAGTTACTGCAACATTTAATTTATACAATGGCTTTGACTGA", "aa": "MLAEDNNETKNKALELFKDYFTTIMKATKVNDKVHYDPSFINEFYGSISNLFEEHDLEPSVEIESNKIRIVKEDKDVIKDKTVTATFNLYNGFD", "aa_hexdigest": "848cd1d2dea6d3e72bd1e49649befdab", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10959.119499999986, "isoelectric_point": 4.762050437927246 }, "id": "GPKLONFDEH_375", "locus": "GCA000006625_00342" }, { "type": "cds", "sequence": "contig_1", "start": 211638, "stop": 211877, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026EF6BA9" ], "nt": "GTGTTTACTAAGGTATTTAATTTAGATAAAGAACAAAAATATCATATTAACAATGTAATGTTCAGCCATAAATTAATTCCCAATTTATTGAAAGCATTGGATATAAAAAACAATACAACTAATGCCGAATTAATTAAAAAAATTAAAGAAAATATGACTGCTATTGATGAAAAAACACAAACAAATTTAAATACAAATTACATTCCTTTGTCACTTACAGAAACAAATAATAAAAAATAA", "aa": "MFTKVFNLDKEQKYHINNVMFSHKLIPNLLKALDIKNNTTNAELIKKIKENMTAIDEKTQTNLNTNYIPLSLTETNNKK", "aa_hexdigest": "f949b614e53f7f68e89221192ae07cb0", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0026EF6BA9", "query_cov": 0.9746835443037974, "subject_cov": 1.0, "identity": 0.74, "score": 105.0, "evalue": 1.95e-28, "valid": false, "uniref50_id": "UniRef50_UPI0026EF6BA9" }, "pscc": { "uniref50_id": "UniRef50_UPI0026EF6BA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026EF6BA9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9222.579099999992, "isoelectric_point": 9.436511421203612 }, "id": "GPKLONFDEH_376", "locus": "GCA000006625_00343" }, { "type": "cds", "sequence": "contig_1", "start": 212055, "stop": 212630, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "TTGAAATCAAAAAAGGTTTATTTTGATTTTGAATCAATTAATTCAGCAATTCGCGCTGTTGATAATTCGTTTCCTTTTACCCAAACTGTTACACAAAATTCTATTATTATTGATGATGGAAGTTGTGATATTAAAAATTTAAAATGCACTAATATGATTATTGATCCACGTGAAATTAATAATGAATGGTTTAAAGCAATTATTGATAAAATTCACCAGGGATTAGAATATTCATATATTGTATACAACAAATCTTTTGAAGCTTCGCGTCTTAAAGAAATGGCTGAATTCATTAATGAAGAAATATATGAATTAAAGGTTAGCGAAATAATTAAAAACATGTATGATTTAGCTGATTTTTTTATAGTTTCAATGGGAAAGCAATTAATTGTAATTCCTGAATTAAAAGGTTTTTATTCAATTAAAAAAGTCTTACCAATTATTGAAAAAGAATTTCCACAAATTTATCACGATGTTAATTGTTTAAACTACAAAGAACTAAGTGTGGGTAATGGTTTGGTTTGTCAAACAAAAACAACAAAACGTTTTTTCAATACAATTACTGATCTAGAATGA", "aa": "MKSKKVYFDFESINSAIRAVDNSFPFTQTVTQNSIIIDDGSCDIKNLKCTNMIIDPREINNEWFKAIIDKIHQGLEYSYIVYNKSFEASRLKEMAEFINEEIYELKVSEIIKNMYDLADFFIVSMGKQLIVIPELKGFYSIKKVLPIIEKEFPQIYHDVNCLNYKELSVGNGLVCQTKTTKRFFNTITDLE", "aa_hexdigest": "79acd12e0cbd08db906a9b7dc879c14c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 137, "aa_cov": 0.7068062827225131, "hmm_cov": 0.9197080291970803, "evalue": 9.323772559752282e-29, "score": 95.87157440185547, "start": 6, "stop": 140 } ], "seq_stats": { "molecular_weight": 22260.45050000002, "isoelectric_point": 5.369034004211425 }, "id": "GPKLONFDEH_377", "locus": "GCA000006625_00344" }, { "type": "cds", "sequence": "contig_1", "start": 212844, "stop": 214004, "strand": "-", "frame": 2, "gene": null, "product": "putative protein UU173", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX0", "UniRef:UniRef90_Q9PQX0" ], "nt": "ATGCGCACAGGGAAATACATTACTAAATATGATTACATTGGTTATTATACAAAGCAAAAAGCAATGTGGTTTTTCACTAATGCTGAGATTAAAGCTAAAATCGACTTATTGTTAAAAAATGTTAGTTCTTATGATTTTGAGGATTTAGATGATGAAGAAGAACATGATTATGAAATTGATGCATATGAAATTTATAAGGAACAAACTGATTTTCTTTCACAATTAGAAATTAATAAAAATATAGATGTGGACAATCCTAAAATTGCTGAAGGTATTTTATTAGATAAAGCATCACAAGAAGATATTAAAAAAACTTATCATTACATTAAGAAAATTATTAATTTCGAAACTGATCCTTTATTTAAAAACAAATCATTAGAAGAGCTTGCTAATTTAACTTTAGAATTAATTGATAAAAATGATAAAATCATTTTTTTTCAACCTGTTTTTATTAATGATAAATTAATTACTAAACCCGATTGTTTTATCAAAAAGAACGATGAATTTATTGTTATCGAAACAAAAGGGACAAGTTCAATTAAAAAACACCATATTTTAGATGTATTTTACCAAGCACAAGTTATCTCAAAACACCCTTATTTTATAAATAAATGTATAAGTTATCAAATGTGCATTGTTGATTATGTTAAATTAAAAAAAAATCAAGTAAATTTTACAATTTCTCCTTATTATAATTATCAAAAAAGCGTAGTTTTATCTTTACCACCAAGTGCTGCAAATCAATTTAATAAATTTGAAATTAATGAATTAAAACGTAAACGTAAGCGTGGAGAAGCAATTTATTTAAAAAAAGACGAAAATAAAAAATATTATGAAGATATTGATGCTTATGATCATTGTAAAGGTATTTTAATAGATGATTTAATCGATGGAACCTATAGTAGCATAAATGATTATAAGGAAGCCAAAAAAATTTATGATTACAAAACAGAAGATTTAAAAGATGATGTACGTTTTAATAAAACCATGGATGAATTAATTAAAGGCATTGAAAAATTACACAATGATTTTGATGAAGTAATCAGCGAATTATTAGTTCATAAACAAAATTTATTAGCTTTGCCTATCGAACAAAGAATTCCTAACTATTTTATACCATCATATAATGATAAGGGTGACTATAAGGATAATGATTTTTGA", "aa": "MRTGKYITKYDYIGYYTKQKAMWFFTNAEIKAKIDLLLKNVSSYDFEDLDDEEEHDYEIDAYEIYKEQTDFLSQLEINKNIDVDNPKIAEGILLDKASQEDIKKTYHYIKKIINFETDPLFKNKSLEELANLTLELIDKNDKIIFFQPVFINDKLITKPDCFIKKNDEFIVIETKGTSSIKKHHILDVFYQAQVISKHPYFINKCISYQMCIVDYVKLKKNQVNFTISPYYNYQKSVVLSLPPSAANQFNKFEINELKRKRKRGEAIYLKKDENKKYYEDIDAYDHCKGILIDDLIDGTYSSINDYKEAKKIYDYKTEDLKDDVRFNKTMDELIKGIEKLHNDFDEVISELLVHKQNLLALPIEQRIPNYFIPSYNDKGDYKDNDF", "aa_hexdigest": "bdca69183c29de5658b716351c03283e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 212541, "stop": 214004, "observations": { "mutation": [ 212846 ], "stop": [ 212846 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQX0", "query_cov": 1.0, "subject_cov": 0.5594202898550724, "identity": 1.0, "score": 769.0, "evalue": 4.09e-276, "gene_start": 1, "gene_end": 386, "reference_start": 1, "reference_end": 386, "reference_sequence": "MRTGKYITKYDYIGYYTKQKAMWFFTNAEIKAKIDLLLKNVSSYDFEDLDDEEEHDYEIDAYEIYKEQTDFLSQLEINKNIDVDNPKIAEGILLDKASQEDIKKTYHYIKKIINFETDPLFKNKSLEELANLTLELIDKNDKIIFFQPVFINDKLITKPDCFIKKNDEFIVIETKGTSSIKKHHILDVFYQAQVISKHPYFINKCISYQMCIVDYVKLKKNQVNFTISPYYNYQKSVVLSLPPSAANQFNKFEINELKRKRKRGEAIYLKKDENKKYYEDIDAYDHCKGILIDDLIDGTYSSINDYKEAKKIYDYKTEDLKDDVRFNKTMDELIKGIEKLHNDFDEVISELLVHKQNLLALPIEQRIPNYFIPSYNDKGDYKDNDFWLELRNLYAYSGYDVIKYSGKILTIKKFGLDNVTKDILGIDILKTYANQTFFNLYKNNQIKIYQAAVDLFAKLKSKKVYFDFESINSAIRAVDNSFPFTQTVTQNSIIIDDGSCDIKNLKCTNMIIDPREINNEWFKAIIDKIHQGLEYSYIVYNKSFEASRLKEMAEFINEEIYELKVSEIIKNMYDLADFFIVSMGKQLIVIPELKGFYSIKKVLPIIEKEFPQIYHDVNCLNYKELSVGNGLVCQTKTTKRFFNTITDLEWEQFVHDARIYCENDVRAMIAVEYFIKKLIDEAKMKNLILS" }, "paralog": false, "identity": 0.997946611909651, "score": 981.0, "evalue": 0.0, "description": "Internal stop codon at 212846. Nonsense mutation around 212846.", "psc": { "uniref90_id": "UniRef90_Q9PQX0", "product": "Uncharacterized protein UU173", "uniref50_id": "UniRef50_Q9PQX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQX0", "UniRef:UniRef50_Q9PQX0" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQX0" ], "product": "Uncharacterized protein UU173" } }, "id": "GPKLONFDEH_378", "locus": "GCA000006625_00345" }, { "type": "cds", "sequence": "contig_1", "start": 214113, "stop": 214307, "strand": "-", "frame": 2, "gene": null, "product": "HAD hydrolase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022B7618D", "UniRef:UniRef90_UPI0022B7618D" ], "nt": "ATGGCTATGCGCTATTTATCCTCATATTATGGAATTCCATTAGATCGTTGTATAGCTTTCGGCGATGGTGAAAATGATTTGACAATGCTAAACCATGCTGGATATGGCTTTGCCATGAAAAATGGTACACGTCCCGCTAAATTACTAGCACGTCACATTACTAAACACACAAACGTAGAAAATGGTGTTGTTTGA", "aa": "MAMRYLSSYYGIPLDRCIAFGDGENDLTMLNHAGYGFAMKNGTRPAKLLARHITKHTNVENGVV", "aa_hexdigest": "e1884759a314259f1fb411fecff262b6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 214080, "stop": 214508, "observations": { "mutation": [ 214115, 214373 ], "stop": [ 214115, 214373 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0022B7618D", "query_cov": 1.0, "subject_cov": 0.4507042253521127, "identity": 1.0, "score": 136.0, "evalue": 6.51e-40, "gene_start": 1, "gene_end": 64, "reference_start": 68, "reference_end": 131, "reference_sequence": "MTKENDINNLIRDANALLLNVNDKALFDSMVYHIKQIAPTLAVRSWSARVDDQFEVIEINSSFADKAMAMRYLSSYYGIPLDRCIAFGDGENDLTMLNHAGYGFAMKNGTRPAKLLARHITKHTNVENGVVWELEYVLNENK" }, "paralog": false, "identity": 0.9788732394366197, "score": 276.0, "evalue": 1.15e-97, "description": "Internal stop codon at 214115, 214373. Nonsense mutation around 214115, 214373.", "psc": { "uniref90_id": "UniRef90_UPI0022B7618D", "product": "HAD hydrolase family protein", "uniref50_id": "UniRef50_UPI0022B7618D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0022B7618D", "UniRef:UniRef50_UPI0022B7618D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0022B7618D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022B7618D" ], "product": "HAD hydrolase family protein" } }, "id": "GPKLONFDEH_379", "locus": "GCA000006625_00346" }, { "type": "cds", "sequence": "contig_1", "start": 214371, "stop": 214526, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGAAAAAAATAAATTGACTAAGGAAAATGATATTAACAACCTTATTAGAGATGCTAATGCCCTTTTATTAAATGTCAACGATAAAGCTTTATTTGATTCTATGGTTTATCATATTAAACAAATTGCTCCCACTTTAGCGGTTCGTAGCTGA", "aa": "MNEKNKLTKENDINNLIRDANALLLNVNDKALFDSMVYHIKQIAPTLAVRS", "aa_hexdigest": "34895fd00a13c4de98010f5009120ae9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5811.6478, "isoelectric_point": 8.120453834533691 }, "id": "GPKLONFDEH_380", "locus": "GCA000006625_00347" }, { "type": "cds", "sequence": "contig_1", "start": 214566, "stop": 214955, "strand": "-", "frame": 2, "gene": null, "product": "HAD-IIB family hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022B758C4", "UniRef:UniRef90_UPI0022B758C4" ], "nt": "ATGGGAAAAAACAACAAATATTTAATTGCTTGTGATCTAGATGGAACACTTTTAAATAATGATAGTGATATATCAGAAAAAACAATTGAGGGAATTAAAAAAATCATCAATCAAGGCCATATTTTCTGTATTGTAACAGGTCGTCCCTTACGTGGATCAATCCATATTTATGAAAAATTAGGATTAACAACTTTAATGGCTAATTATAATGGCTCTTATATTTCAAACCCTTCCGATAAAAAATTCGCGCCAATTAATATGACATTTAGTAAAGATATTGTAAGCGCAATTTTAAAAAATCGATTTATTGCTAAAAATATTGTTAATGCTGTTGTTGAAGCTGATAATGTTGCTACCATTTTAAACAGTAATATGGATTTAAATATTTGA", "aa": "MGKNNKYLIACDLDGTLLNNDSDISEKTIEGIKKIINQGHIFCIVTGRPLRGSIHIYEKLGLTTLMANYNGSYISNPSDKKFAPINMTFSKDIVSAILKNRFIAKNIVNAVVEADNVATILNSNMDLNI", "aa_hexdigest": "e941781da234dd461985c9f43d8a929b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 214451, "stop": 214955, "observations": { "insertion": [ 214517 ], "mutation": [ 214568 ], "stop": [ 214507, 214568 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0022B758C4", "query_cov": 1.0, "subject_cov": 0.7724550898203593, "identity": 1.0, "score": 251.0, "evalue": 4.32e-84, "gene_start": 1, "gene_end": 129, "reference_start": 1, "reference_end": 129, "reference_sequence": "MGKNNKYLIACDLDGTLLNNDSDISEKTIEGIKKIINQGHIFCIVTGRPLRGSIHIYEKLGLTTLMANYNGSYISNPSDKKFAPINMTFSKDIVSAILKNRFIAKNIVNAVVEADNVATILNSNMDLNIWKSFIDIFHVPIRDMNEKINWLRKMILTTLLEMLMPFY" }, "paralog": false, "identity": 0.9880239520958084, "score": 319.0, "evalue": 2.75e-113, "description": "Internal stop codon at 214507, 214568. Frameshift due to insertion around 214517. Nonsense mutation around 214568.", "psc": { "uniref90_id": "UniRef90_UPI0022B758C4", "product": "HAD-IIB family hydrolase", "uniref50_id": "UniRef50_UPI0022B758C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0022B758C4", "UniRef:UniRef50_UPI0022B758C4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0022B758C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022B758C4" ], "product": "HAD-IIB family hydrolase" } }, "id": "GPKLONFDEH_381", "locus": "GCA000006625_00348" }, { "type": "cds", "sequence": "contig_1", "start": 215089, "stop": 215982, "strand": "-", "frame": 1, "gene": "trpS", "product": "tryptophan--tRNA ligase", "db_xrefs": [ "COG:COG0180", "COG:J", "EC:6.1.1.2", "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436", "SO:0001217", "UniRef:UniRef50_Q9PQW8", "UniRef:UniRef90_Q9PQW8" ], "nt": "ATGAAAAGACTAATTTCAGGAATTCAACCAACTAATAATTTAACATTAGGAAATTATTTAGGTGCAATTAAAAACTTTGTTGATTTACAAGATGATTATGAAGTGTTTTTATTTGTTGCGGATTTACATTCCTTAACACCAAATATTTTTGATAACACTGATTTTTCTACTACTAAACGTCAGATTATCGCGACATATTTAGCTGCTGGTATTGATCCTAAAAAAACATGTCTATTTTACCAATCAGATATCTTATCGATTCCATTATTAGCTCACATATTATTATGTTCAACAAGTATTGGTGAACTAACGCGTATGACACAATTTAAAGATAAAAGTATTAAAGCAACAAAGATGGCTAATAACACTGAAATGATTCCTTCTGGTTTATTAACATATCCCACATTAATGGCAGCAGATATTTTAACTTTTAATGCAGATGTTGTTCCTGTTGGCCAAGATCAAAAACAACATTTGGAATTAACAAGAACGCTTGCTGACCGTTTTAATAAAAGATATGGTAATACTTTTAAATTGCCTCAAGTTTATATTCCTAAAATAGGGGCAAAAATTATGGATTTATTAGATCCTAATATTAAAATGTCAAAATCTTCTAAAAACCTTAAGGGTGTGATTTTTTTAAATGATTCAAAGGATATAATTTTTAAAAAAATTAAAGGAGCTTTAACAGATAATTTAAACAAAGTTAAATATGATTTAACATTACAACCAGGAGTAAGTAATTTAATGACAATTTATGCTTGTTTAACAAATCTAAGCTTTAAAGAAATCGAACTAAAATACCAACAACAAAACTATGGTGTTTTTAAAAATGATTTAGCCAATATCGTTGCTGATTTTTTAGAAAAGCTGCAACAAAAAATTTCTTATTGA", "aa": "MKRLISGIQPTNNLTLGNYLGAIKNFVDLQDDYEVFLFVADLHSLTPNIFDNTDFSTTKRQIIATYLAAGIDPKKTCLFYQSDILSIPLLAHILLCSTSIGELTRMTQFKDKSIKATKMANNTEMIPSGLLTYPTLMAADILTFNADVVPVGQDQKQHLELTRTLADRFNKRYGNTFKLPQVYIPKIGAKIMDLLDPNIKMSKSSKNLKGVIFLNDSKDIIFKKIKGALTDNLNKVKYDLTLQPGVSNLMTIYACLTNLSFKEIELKYQQQNYGVFKNDLANIVADFLEKLQQKISY", "aa_hexdigest": "84a863593e94bca8f8d47bc79b66d7bb", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_Q9PQW8", "query_cov": 1.0, "subject_cov": 0.8918918918918919, "identity": 1.0, "score": 584.0, "evalue": 3.15e-210, "valid": true, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_Q9PQW8", "cog_id": "COG0180", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW8" ], "product": "Tryptophan--tRNA ligase" }, "genes": [ "trpS" ], "id": "GPKLONFDEH_382", "locus": "GCA000006625_00349" }, { "type": "cds", "sequence": "contig_1", "start": 216107, "stop": 216493, "strand": "+", "frame": 2, "gene": "arsC", "product": "putative protein UU176", "db_xrefs": [ "COG:COG1393", "COG:P", "GO:0016491", "KEGG:K16509", "RefSeq:WP_006688944.1", "SO:0001217", "UniParc:UPI00001393E2", "UniRef:UniRef100_Q9PQW7", "UniRef:UniRef50_Q9PQW7", "UniRef:UniRef90_Q9PQW7" ], "nt": "ATGATTTACGTTTTATACAGTCCTAATTGCGCAGTGTGTAAAAAAGTTGTGCGATTTTTCCGAAATAATCAAATTGAAATTACCAAAATTATTATTGGTGAAGATAAAATAGAACGTTCTATGTTGTTAGATATTTTATCTCTTTGTGAAGATGGCTTTGGAACAATTATATCTTTTAAGACAGAATCTTCAAAACGCTTGAATATTACTTCAAAAACTTTTTTAGATTTATCTACAAAAGAATTATTAAATTTAATTCAAAATGATTTAAATTTAATTCGTCGCCCTTTAATCTATCAAACAAAAAACAATCGACCATATCGTTTACAAATTGGTTATGATTCAGAAGAAATTGAAATTTTTAAACGAGCAGTGCATGAAAAATAA", "aa": "MIYVLYSPNCAVCKKVVRFFRNNQIEITKIIIGEDKIERSMLLDILSLCEDGFGTIISFKTESSKRLNITSKTFLDLSTKELLNLIQNDLNLIRRPLIYQTKNNRPYRLQIGYDSEEIEIFKRAVHEK", "aa_hexdigest": "f0967a88c83258b88b7d3528daa4db81", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001393E2", "ncbi_nrp_id": "WP_006688944.1", "uniref100_id": "UniRef100_Q9PQW7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393E2", "RefSeq:WP_006688944.1", "UniRef:UniRef100_Q9PQW7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQW7", "uniref90_id": "UniRef90_Q9PQW7" }, "psc": { "uniref90_id": "UniRef90_Q9PQW7", "gene": "ArsC", "product": "Uncharacterized protein UU176", "uniref50_id": "UniRef50_Q9PQW7", "cog_id": "COG1393", "cog_category": "P", "kegg_orthology_id": "K16509", "go_ids": [ "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW7" ], "product": "Uncharacterized protein UU176" }, "genes": [ "arsC" ], "id": "GPKLONFDEH_383", "locus": "GCA000006625_00350" }, { "type": "cds", "sequence": "contig_1", "start": 216483, "stop": 216686, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAATAAGCCAATTTGTTTTTATGATATCTATTGTTTTAAACCACAAGAATGTTTTAAAAGAAATGATATTAATTTGTTAGAAGATAAACTAAAAAGATATTCAAAAATAACATTTTTACGAAATAAAGAAAATCCTGAAATTATTTTTTTATTAGGTGGAGATGGATCGTTTATCAATTTTATTAACCAACAATGA", "aa": "MKNNKPICFYDIYCFKPQECFKRNDINLLEDKLKRYSKITFLRNKENPEIIFLLGGDGSFINFINQQ", "aa_hexdigest": "f353284c2e324bd4b08f7fbbd622573d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8068.2900999999965, "isoelectric_point": 8.8624849319458 }, "id": "GPKLONFDEH_384", "locus": "GCA000006625_00351" }, { "type": "cds", "sequence": "contig_1", "start": 216792, "stop": 217295, "strand": "+", "frame": 3, "gene": "nadK", "product": "NAD kinase", "db_xrefs": [ "COG:COG0061", "COG:H", "EC:2.7.1.23", "GO:0003951", "GO:0005524", "GO:0005737", "GO:0006741", "GO:0019674", "GO:0046872", "GO:0051287", "KEGG:K00858", "SO:0001217", "UniRef:UniRef50_Q9PQW6", "UniRef:UniRef90_Q9PQW6" ], "nt": "ATGTATTATAATCCTTTATTACTTAAAGTAAGTATTAATAACCAAAATTTTTTTTATTGTTTGAATGAGTTAAGTTTATTTTCTAATGAGTTAGTGTCTTTTGATATTAGCATAAATGATTATCCATATGAAAAATTTCGTGGCTCTGGTTTGTTATTTGTAACACCAAGCGGATCAACTGGCAAAAACAAAACAGCTTTTGGTCCCATTATTTTTAATAATCATGAGAATTTTATTATGACAGAGATTTTTCCTGTTAATCATTTAAAATATTCAAGTTTAAATGCCCCTGTGGTTTTTAGAAAAGATTATAAAATCAGTTTAACAAATATTAAATTTAAAAAATCTTTTAGTGTTGCTATTGATGGGAATATCATTAATTTTAGCGATAAAATTAATGATATTAAAGTAGAAACAATCCAAGCTTCATCAAAAATTCATGGTTTAAATAATTTTAAGAAATACATTGATAAATTAAACAAATCTTTTATTAAAGGAGAATAA", "aa": "MYYNPLLLKVSINNQNFFYCLNELSLFSNELVSFDISINDYPYEKFRGSGLLFVTPSGSTGKNKTAFGPIIFNNHENFIMTEIFPVNHLKYSSLNAPVVFRKDYKISLTNIKFKKSFSVAIDGNIINFSDKINDIKVETIQASSKIHGLNNFKKYIDKLNKSFIKGE", "aa_hexdigest": "52772a0a3d2ac2f0fe0dd98d9065f35d", "start_type": "ATG", "rbs_motif": null, "genes": [ "nadK" ], "truncated": "5-prime", "pseudogene": { "start": 216492, "stop": 217295, "observations": { "mutation": [ 216684 ], "stop": [ 216684 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQW6", "query_cov": 1.0, "subject_cov": 0.6185185185185185, "identity": 1.0, "score": 331.0, "evalue": 2.04e-113, "gene_start": 1, "gene_end": 167, "reference_start": 104, "reference_end": 270, "reference_sequence": "MKNNKPICFYDIYCFKPQECFKRNDINLLEDKLKRYSKITFLRNKENPEIIFLLGGDGSFINFINQQWKKNVKIVGINYGQLGFYSSYDSIKTINLDEIIDENMYYNPLLLKVSINNQNFFYCLNELSLFSNELVSFDISINDYPYEKFRGSGLLFVTPSGSTGKNKTAFGPIIFNNHENFIMTEIFPVNHLKYSSLNAPVVFRKDYKISLTNIKFKKSFSVAIDGNIINFSDKINDIKVETIQASSKIHGLNNFKKYIDKLNKSFIKGE" }, "paralog": false, "identity": 0.9962546816479401, "score": 540.0, "evalue": 4.24e-198, "description": "Internal stop codon at 216684. Nonsense mutation around 216684.", "psc": { "uniref90_id": "UniRef90_Q9PQW6", "gene": "nadK", "product": "NAD kinase", "ec_ids": [ "2.7.1.23" ], "uniref50_id": "UniRef50_Q9PQW6", "cog_id": "COG0061", "cog_category": "H", "kegg_orthology_id": "K00858", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQW6", "EC:2.7.1.23", "UniRef:UniRef50_Q9PQW6", "COG:COG0061", "COG:H", "KEGG:K00858", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW6" ], "product": "NAD kinase" } }, "id": "GPKLONFDEH_385", "locus": "GCA000006625_00352" }, { "type": "cds", "sequence": "contig_1", "start": 217298, "stop": 217678, "strand": "+", "frame": 2, "gene": "ptsG2", "product": "Putative phosphotransferase enzyme IIB component UU178", "db_xrefs": [ "COG:COG1264", "COG:G", "EC:2.7.1.-", "GO:0008982", "GO:0009401", "GO:0016020", "RefSeq:WP_006688859.1", "SO:0001217", "UniParc:UPI00001393EA", "UniRef:UniRef100_Q9PQW5", "UniRef:UniRef50_Q9PQW5", "UniRef:UniRef90_Q9PQW5" ], "nt": "ATGCAAAATTTAAATAATAAGCAAAAAACATTAATTATTTTTTTAGGTATTATTACCTTTGGAATTTTTATTATTTACTTTTTTACAAAAGCTAAAAAAGTATCTCAAATTAGAAACACTCATTTAATAGTATCTTCAAATATTCCTTTTAGTTTAAATGATTTTTATAATTGTATTGGTTCAAAAAATAATTTAGTTAACGTTGATGCGACAATTAATACATTAAAAATTGAATTAAAAGAAATTAACGCATTGAATAATGAAAAATTAAAGGTTTTAGGTGCAAAAGGGATTATGTGCAATCAAACAAAAATTTCTATTATTTTTGGTGATTTTTGTTTGGAACTTAAAGAATTAATTAAAAAAGATTTATTTTCATAG", "aa": "MQNLNNKQKTLIIFLGIITFGIFIIYFFTKAKKVSQIRNTHLIVSSNIPFSLNDFYNCIGSKNNLVNVDATINTLKIELKEINALNNEKLKVLGAKGIMCNQTKISIIFGDFCLELKELIKKDLFS", "aa_hexdigest": "9230aada5e140e9b7cbbd6e4e87bf9b1", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00001393EA", "ncbi_nrp_id": "WP_006688859.1", "uniref100_id": "UniRef100_Q9PQW5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393EA", "RefSeq:WP_006688859.1", "UniRef:UniRef100_Q9PQW5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQW5", "uniref90_id": "UniRef90_Q9PQW5" }, "psc": { "uniref90_id": "UniRef90_Q9PQW5", "gene": "PtsG2", "product": "Putative phosphotransferase enzyme IIB component UU178", "ec_ids": [ "2.7.1.-" ], "uniref50_id": "UniRef50_Q9PQW5", "cog_id": "COG1264", "cog_category": "G", "go_ids": [ "GO:0016020", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW5" ], "product": "Putative phosphotransferase enzyme IIB component UU178" }, "genes": [ "ptsG2" ], "id": "GPKLONFDEH_386", "locus": "GCA000006625_00353" }, { "type": "cds", "sequence": "contig_1", "start": 217759, "stop": 217980, "strand": "+", "frame": 1, "gene": null, "product": "putative protein UU179", "db_xrefs": [ "RefSeq:WP_010891702.1", "SO:0001217", "UniParc:UPI00001393F0", "UniRef:UniRef100_Q9PQW4", "UniRef:UniRef50_Q9PQW4", "UniRef:UniRef90_Q9PQW4" ], "nt": "ATGTTAATTAAACCCGCGAAACGCAGTGAAAAAATTTATACAATTATTGCTATAAAAGATAGTGTTGTAACTTTAAAAAATGATTATCAAGACGAAAAAAATATTCAAATTTACGAGTTTGATTTGCAGAATATTATTCCAGAAGTTGGTAAATTTATTAATCTAATTAAATACGATCAACAAGGTTGTTCAGTATTTGAAGAATATGATAAAAACATTTAA", "aa": "MLIKPAKRSEKIYTIIAIKDSVVTLKNDYQDEKNIQIYEFDLQNIIPEVGKFINLIKYDQQGCSVFEEYDKNI", "aa_hexdigest": "e3c0fdda5b47fd6722d2ee5d8b81dfaa", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00001393F0", "ncbi_nrp_id": "WP_010891702.1", "uniref100_id": "UniRef100_Q9PQW4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393F0", "RefSeq:WP_010891702.1", "UniRef:UniRef100_Q9PQW4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQW4", "uniref90_id": "UniRef90_Q9PQW4" }, "psc": { "uniref90_id": "UniRef90_Q9PQW4", "product": "Uncharacterized protein UU179", "uniref50_id": "UniRef50_Q9PQW4" }, "pscc": { "uniref50_id": "UniRef50_Q9PQW4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW4" ], "product": "Uncharacterized protein UU179" }, "genes": [], "id": "GPKLONFDEH_387", "locus": "GCA000006625_00354" }, { "type": "cds", "sequence": "contig_1", "start": 218177, "stop": 218461, "strand": "-", "frame": 3, "gene": "rlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "db_xrefs": [ "COG:COG1576", "COG:J", "EC:2.1.1.177", "GO:0005737", "GO:0070038", "KEGG:K00783", "SO:0001217", "UniRef:UniRef50_Q9PQW3", "UniRef:UniRef90_Q9PQW3" ], "nt": "ATGATGATAAAAATTATAAGTGTAGGGAAATTAAAACAAAAAGCTTTTGTTGATCTAATTAATGATTATTTAAAAAGAATTAACCATTATTTAAAATGCCAAGAAATTGTTGTTAGCGATGAACCTGAACCAGTTCAAATTTCAAATAAATCCTTAGAACAAATTAAAAGTAAAGAGGCCTCTAAAATTTTTAAAAATATTAATCAAAATGATTTTGTAATTGCTTTAATTATTGAAAGCAACATTATAAGTAGCGAAACATTAGCTAAAAAAATACAACAATGA", "aa": "MMIKIISVGKLKQKAFVDLINDYLKRINHYLKCQEIVVSDEPEPVQISNKSLEQIKSKEASKIFKNINQNDFVIALIIESNIISSETLAKKIQQ", "aa_hexdigest": "e623f353c4317df63a7609d96bd27e5d", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [ "rlmH" ], "truncated": "5-prime", "pseudogene": { "start": 217982, "stop": 218461, "observations": { "mutation": [ 218179 ], "stop": [ 218179 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQW3", "query_cov": 1.0, "subject_cov": 0.5911949685534591, "identity": 1.0, "score": 176.0, "evalue": 8.1e-55, "gene_start": 1, "gene_end": 94, "reference_start": 1, "reference_end": 94, "reference_sequence": "MMIKIISVGKLKQKAFVDLINDYLKRINHYLKCQEIVVSDEPEPVQISNKSLEQIKSKEASKIFKNINQNDFVIALIIESNIISSETLAKKIQQWLNTFSHDICFIIGGSNGLHESIYERANYHLSMSKMTFAHGLAKVMLCEQIYRALSILNNGKYHK" }, "paralog": false, "identity": 0.9937106918238994, "score": 310.0, "evalue": 1.82e-110, "description": "Internal stop codon at 218179. Nonsense mutation around 218179.", "psc": { "uniref90_id": "UniRef90_Q9PQW3", "gene": "rlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_Q9PQW3", "cog_id": "COG1576", "cog_category": "J", "kegg_orthology_id": "K00783", "go_ids": [ "GO:0005737", "GO:0070038" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQW3", "EC:2.1.1.177", "UniRef:UniRef50_Q9PQW3", "COG:COG1576", "COG:J", "KEGG:K00783", "GO:0005737", "GO:0070038" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW3" ], "product": "Ribosomal RNA large subunit methyltransferase H" } }, "id": "GPKLONFDEH_388", "locus": "GCA000006625_00355" }, { "type": "cds", "sequence": "contig_1", "start": 218524, "stop": 219006, "strand": "+", "frame": 1, "gene": "tpiA", "product": "Triosephosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_Q9PQW2", "UniRef:UniRef90_Q9PQW2" ], "nt": "ATGAAATATATTATTGCTAATTTTAAAATGAATGCAACACAAGAATTAATTAATCATTTTTTAAATAATTTAACGTTATTTGATGAGCAAAAAATTATTATTGGTTTAGCACCAGGGGATTTATATTTAAAAACATTTGTTAATTTAGCTGAAATAAAAAAAGTCAATTTATATGCTCAAAACCCATCATTACATAATAAAGGACCTTATACAGGTCAAATAAGTTGTTTACAATTATTAGATATTAATATTAAAAATGCTTTAGTTGGGCATAGTGAAATACGTATTGATTTTTCACAATCAATAATTGATCAAAAAATAAAAATATCTATGGATTTATTAGAACAAGTCATCATTTGTGTTGGCGAAACTTTTGATGCTTATAAACAAAATAAATCACTTAATTTTGTTTTAAATCAATTAGCCAACATCATTAATTACAAAGGATTAAAAAAAATTATTATCGCTTATGAACCAATTTGA", "aa": "MKYIIANFKMNATQELINHFLNNLTLFDEQKIIIGLAPGDLYLKTFVNLAEIKKVNLYAQNPSLHNKGPYTGQISCLQLLDINIKNALVGHSEIRIDFSQSIIDQKIKISMDLLEQVIICVGETFDAYKQNKSLNFVLNQLANIINYKGLKKIIIAYEPI", "aa_hexdigest": "80e82917041c50e50b5b820b4ee8d13e", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "tpiA" ], "truncated": "3-prime", "pseudogene": { "start": 218524, "stop": 219240, "observations": { "mutation": [ 219004 ], "stop": [ 219004 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQW2", "query_cov": 1.0, "subject_cov": 0.6722689075630253, "identity": 1.0, "score": 317.0, "evalue": 3.11e-108, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MKYIIANFKMNATQELINHFLNNLTLFDEQKIIIGLAPGDLYLKTFVNLAEIKKVNLYAQNPSLHNKGPYTGQISCLQLLDINIKNALVGHSEIRIDFSQSIIDQKIKISMDLLEQVIICVGETFDAYKQNKSLNFVLNQLANIINYKGLKKIIIAYEPIWAIGTDLELDFKHINYMIEGIKTYLYNCTGINIPILYGGSVNDNNINELCNQKLIDGFLIGNASLDVNVFNKIIDKCK" }, "paralog": false, "identity": 0.9957983193277311, "score": 473.0, "evalue": 3.14e-172, "description": "Internal stop codon at 219004. Nonsense mutation around 219004.", "psc": { "uniref90_id": "UniRef90_Q9PQW2", "gene": "tpiA", "product": "Triosephosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_Q9PQW2", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0005829", "GO:0004807", "GO:0006094", "GO:0046166", "GO:0019563", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQW2", "EC:5.3.1.1", "UniRef:UniRef50_Q9PQW2", "COG:COG0149", "COG:G", "KEGG:K01803", "GO:0005829", "GO:0004807", "GO:0006094", "GO:0046166", "GO:0019563", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW2" ], "product": "Triosephosphate isomerase" } }, "id": "GPKLONFDEH_389", "locus": "GCA000006625_00356" }, { "type": "cds", "sequence": "contig_1", "start": 219052, "stop": 219240, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00121.24" ], "nt": "ATGATCGAAGGAATTAAAACATATTTATATAATTGTACTGGAATAAATATTCCTATTTTATATGGCGGTAGTGTTAATGACAATAATATTAATGAATTGTGCAATCAAAAATTAATCGATGGTTTTTTAATTGGCAATGCTTCATTAGATGTTAATGTTTTTAATAAAATTATCGATAAATGTAAATAA", "aa": "MIEGIKTYLYNCTGINIPILYGGSVNDNNINELCNQKLIDGFLIGNASLDVNVFNKIIDKCK", "aa_hexdigest": "79463bb1de09f4d01690b994973d8395", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00121.24", "name": "TIM", "length": 57, "aa_cov": 0.8709677419354839, "hmm_cov": 1.0, "evalue": 3.2126137319559548e-16, "score": 55.137760162353516, "start": 7, "stop": 60 } ], "seq_stats": { "molecular_weight": 6865.879400000002, "isoelectric_point": 4.927224540710449 }, "id": "GPKLONFDEH_390", "locus": "GCA000006625_00357" }, { "type": "cds", "sequence": "contig_1", "start": 219257, "stop": 219631, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAATAAAAAAATAGGTTTGATAATCATTGATGGATTAGGAATTGGAAAAGAAGATAATACTAATGCTGTTCATTTAGCAAATCCACCAACATTAAATTATTTGATTAATAATTTTCCCACATTACAAATTAGTGCGGCTCAACAAAATATTGGTTTACTTGAAAATCAAGCAGGTAACAGCGAAATTGGTCATTTAACAATTGGTGCGGGCCGTATCATTTTAAATGATAATGCAAATATTAATAATTATATGAAATCCGTTGATTATGAAAGTGCAGTTTTAAGCAATATTAAAAATGAAATTGTTCATATTGTTGGCATGTATTCGAATGGTTTAGTACATAGTAATTATGAACATATTCATTGA", "aa": "MKLNKKIGLIIIDGLGIGKEDNTNAVHLANPPTLNYLINNFPTLQISAAQQNIGLLENQAGNSEIGHLTIGAGRIILNDNANINNYMKSVDYESAVLSNIKNEIVHIVGMYSNGLVHSNYEHIH", "aa_hexdigest": "9b27fea752687f9a894c3e261c472dfe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13529.201999999987, "isoelectric_point": 6.012451362609862 }, "id": "GPKLONFDEH_391", "locus": "GCA000006625_00358" }, { "type": "cds", "sequence": "contig_1", "start": 219845, "stop": 220765, "strand": "+", "frame": 2, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537", "KEGG:K15633", "SO:0001217", "UniRef:UniRef50_Q9PQW1", "UniRef:UniRef90_Q9PQW1" ], "nt": "ATGTTTGTTAAGCAAGATAAAATATGTGAAAAAACATTTTTAGAGGTTGCACAATCAATCGCAAATTCTTATAAAAGTGATGAATTTGTTCAACCTGTAGTTTTTAATGATGATGAAAAATATAATTTAAAACCACATCAAAAAGTGATTTTAGCAAACTATCGTTCAGATCGTATGCGCCAGTTAGCACACTTACTTAAACCTAACAAAAAATTTCATTACAATAATCCGTTTTTAGTTCAAGATGTTAATTTAATTACTTTAGTACCATTCCCTGATGTTGATGCAATAGCTTTATTTGAGAAGCAAAATTTAAATAACACTTTAGGTGATGTTTTTAATAAACACCAAATAAAAGAAGCTCGAATTGCAGAAACAGAAAAATATGCCCATATATCTTTTTTCTTTGATGGCGGTGTTAATAAATATTATTCGACAAAAACACAATATTTGATTCCTTCGCAAAAAGTTGCTACTTATGATTTATGTCCCCAAATGTCAGCACATTTAATTACGAAAACTATTATCGATCATTATTTCGATCATGATGTTTTTATTGTTAATTATGCAAATCCTGATATGGTTGGTCATAGCGGCAATATGCAGCAAACAATTGAAGCAATTCTAAGTGTTGACCATGAAATTCGAAAATTATATGATTTTTTTGAAAAGAATAATGGTGTGTTGATGATTACAGGTGATCATGGAAATGCTGAAATGATGCTTGATGATCAAAAACAAGTAATTACTTCTCACAGTGTTAATGATGTTTGGTTTATTATCACAGATAATAAAGTTATTTTAAATTCGACACAAAAATTTAGTTTAGCAAATATTGCTCCAACTATTTTGGAATATTTAAATATCGCTCAACCAATTCAAATGACAGCAACGAGTATGATAAAAAAGATTTGTGAATAG", "aa": "MFVKQDKICEKTFLEVAQSIANSYKSDEFVQPVVFNDDEKYNLKPHQKVILANYRSDRMRQLAHLLKPNKKFHYNNPFLVQDVNLITLVPFPDVDAIALFEKQNLNNTLGDVFNKHQIKEARIAETEKYAHISFFFDGGVNKYYSTKTQYLIPSQKVATYDLCPQMSAHLITKTIIDHYFDHDVFIVNYANPDMVGHSGNMQQTIEAILSVDHEIRKLYDFFEKNNGVLMITGDHGNAEMMLDDQKQVITSHSVNDVWFIITDNKVILNSTQKFSLANIAPTILEYLNIAQPIQMTATSMIKKICE", "aa_hexdigest": "0fcd017c36cb6d8f58374e4e92c8ec97", "start_type": "ATG", "rbs_motif": null, "genes": [ "gpmI" ], "truncated": "5-prime", "pseudogene": { "start": 219545, "stop": 220765, "observations": { "mutation": [ 219629, 219815 ], "stop": [ 219629, 219815 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQW1", "query_cov": 1.0, "subject_cov": 0.6095617529880478, "identity": 1.0, "score": 615.0, "evalue": 1.97e-219, "gene_start": 1, "gene_end": 306, "reference_start": 197, "reference_end": 502, "reference_sequence": "MKLNKKIGLIIIDGLGIGKEDNTNAVHLANPPTLNYLINNFPTLQISAAQQNIGLLENQAGNSEIGHLTIGAGRIILNDNANINNYMKSVDYESAVLSNIKNEIVHIVGMYSNGLVHSNYEHIHWIIKQIIKNNNQVVLHLISDGRDDYPYGFAQFIEQINALKKQYNIIVKSLSGRYFAMDRDQRWERTQKAFNAMFVKQDKICEKTFLEVAQSIANSYKSDEFVQPVVFNDDEKYNLKPHQKVILANYRSDRMRQLAHLLKPNKKFHYNNPFLVQDVNLITLVPFPDVDAIALFEKQNLNNTLGDVFNKHQIKEARIAETEKYAHISFFFDGGVNKYYSTKTQYLIPSQKVATYDLCPQMSAHLITKTIIDHYFDHDVFIVNYANPDMVGHSGNMQQTIEAILSVDHEIRKLYDFFEKNNGVLMITGDHGNAEMMLDDQKQVITSHSVNDVWFIITDNKVILNSTQKFSLANIAPTILEYLNIAQPIQMTATSMIKKICE" }, "paralog": false, "identity": 0.9950738916256158, "score": 812.0, "evalue": 8.5e-300, "description": "Internal stop codon at 219629, 219815. Nonsense mutation around 219629, 219815.", "psc": { "uniref90_id": "UniRef90_Q9PQW1", "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_Q9PQW1", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQW1", "EC:5.4.2.12", "UniRef:UniRef50_Q9PQW1", "COG:COG0696", "COG:G", "KEGG:K15633", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQW1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQW1" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" } }, "id": "GPKLONFDEH_392", "locus": "GCA000006625_00359" }, { "type": "cds", "sequence": "contig_1", "start": 221307, "stop": 221570, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTTAAATACTTATATTAATCACTATCATTTACCAATAATTAATGATCCACAAGTTGCTTTATTAGATATTAGCAAACACCAAATTAATAATGAAAATTTAACATTTAATCCTTCATACAACTATAATGCCAATCTTTATTTTACAAAAGCAAAATTAATTTATTGTACTTATACAATTATTGGAATAATTTTTATCATTAGTTTTATTTATTATCTAAGTGAAATTATTAAACGATGTTTATTAGTTAATGACAATTGA", "aa": "MILNTYINHYHLPIINDPQVALLDISKHQINNENLTFNPSYNYNANLYFTKAKLIYCTYTIIGIIFIISFIYYLSEIIKRCLLVNDN", "aa_hexdigest": "48e42a5a31d891c2cdac8e1167d29e36", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10277.842599999993, "isoelectric_point": 6.801092338562011 }, "id": "GPKLONFDEH_393", "locus": "GCA000006625_00360" }, { "type": "cds", "sequence": "contig_1", "start": 221790, "stop": 222638, "strand": "+", "frame": 3, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "COG:COG0148", "COG:G", "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0006096", "GO:0009986", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_Q9PQV9", "UniRef:UniRef90_Q9PQV9" ], "nt": "ATGAAAATAATTAATTTATTAGCTTATCAAATTCTTGATTCGCGCGGCCAACCAACAGTAGCTGTTAAATTATTTTTAGAGAACGATCAAAGTGTAATTGCAATGGTTCCATCAGGCGCTTCCACTGGAGCAAAGGAAGCATTAGAATTAAGAGATGGTGATGTAAATTATTTTTTTAATAAGTCTGTTAAATTAGCAATTCAAAATATTAATAATATTATTCGCCCTCATCTAATTAATAAAAATGTTTTAAATTTTTTTGAATTAGATAATTTATTAATTAATCTAGATGGTACTGAAAATAAAAGTAAATTAGGTGCTAATGCTTTATTAGGTGTTTCTATAGCGATTGTTAAAGCGGGAGCAATAGCCGCTTCTAAACCTTTATATCAATATATTAAAGAAGACTTAATGCATAATTATGATGTAAACTATTATGCTCCAATTCCGTTAATGAATTTTATTAATGGTGGGGCTCACGCAGATAATGATTTAGATATTCAAGAATTTATGATTGTTCCATTAAATGCAATTTCATTTTCTCAAGCGATTCAAATTGGATCAGAAATTTTTCATCAATTAGATAAACTTTTAAAAAGCAATCATTTAAGTACAACAAAGGGTGATGAAGGCGGTTTTGCTCCAATGTTAAAAAATAATTATGTGACTTTAGAACTATTAGTTCATGCTATTAAAAAAGCACATTACCTTCCTTCAAAAACACAAGGAGTTTGTCTTGCCTTAGATGTCGCAGCTTCTGAATTGTATGAAAACGGAAAATATTTTTTTAAAAAGTCTTCATCTCATAACATTACTCTTGAACAAACATCATTCAGTAGTGATGAATGA", "aa": "MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEALELRDGDVNYFFNKSVKLAIQNINNIIRPHLINKNVLNFFELDNLLINLDGTENKSKLGANALLGVSIAIVKAGAIAASKPLYQYIKEDLMHNYDVNYYAPIPLMNFINGGAHADNDLDIQEFMIVPLNAISFSQAIQIGSEIFHQLDKLLKSNHLSTTKGDEGGFAPMLKNNYVTLELLVHAIKKAHYLPSKTQGVCLALDVAASELYENGKYFFKKSSSHNITLEQTSFSSDE", "aa_hexdigest": "41e75c27ce1dbf706a866b914bc7be4a", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0026067ABB", "query_cov": 0.8900709219858156, "subject_cov": 0.8508474576271187, "identity": 0.518, "score": 239.0, "evalue": 4.98e-75, "valid": false, "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_A0A838WLF1", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "genes": [ "eno" ], "truncated": "3-prime", "pseudogene": { "start": 221790, "stop": 222941, "observations": { "mutation": [ 222636, 222648, 222711 ], "stop": [ 222636, 222648, 222711 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQV9", "query_cov": 1.0, "subject_cov": 0.6409090909090909, "identity": 1.0, "score": 549.0, "evalue": 8.59e-195, "gene_start": 1, "gene_end": 282, "reference_start": 1, "reference_end": 282, "reference_sequence": "MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEALELRDGDVNYFFNKSVKLAIQNINNIIRPHLINKNVLNFFELDNLLINLDGTENKSKLGANALLGVSIAIVKAGAIAASKPLYQYIKEDLMHNYDVNYYAPIPLMNFINGGAHADNDLDIQEFMIVPLNAISFSQAIQIGSEIFHQLDKLLKSNHLSTTKGDEGGFAPMLKNNYVTLELLVHAIKKAHYLPSKTQGVCLALDVAASELYENGKYFFKKSSSHNITLEQTSFSSDEWIKYWSKLVSMFPIISIEDCFEENDWNSFALFLKNNPHIQVVGDDLYCTNLKYLQKGIDFKATNAILIKPNQIGTISETLDVIKFAQKNNINTIISHRSGETEDTFIADLAIGVGAGQIKTGSLSRSERIAKYNRILEIEQELKDKLIYEPSKFFKFR" }, "paralog": false, "identity": 0.9921671018276762, "score": 744.0, "evalue": 4.12e-274, "description": "Internal stop codon at 222636, 222648, 222711. Nonsense mutation around 222636, 222648, 222711.", "psc": { "uniref90_id": "UniRef90_Q9PQV9", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_Q9PQV9", "cog_id": "COG0148", "cog_category": "G", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0006096", "GO:0009986" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQV9", "EC:4.2.1.11", "UniRef:UniRef50_Q9PQV9", "COG:COG0148", "COG:G", "KEGG:K01689", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0006096", "GO:0009986" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV9" ], "product": "Enolase" } }, "id": "GPKLONFDEH_394", "locus": "GCA000006625_00361" }, { "type": "cds", "sequence": "contig_1", "start": 222756, "stop": 223112, "strand": "+", "frame": 3, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E9FKC7" ], "nt": "GTGGTTGGTGATGATTTATATTGCACAAACTTAAAGTATCTACAAAAAGGAATTGATTTTAAAGCAACAAATGCTATTTTAATTAAACCTAATCAAATTGGTACAATTAGTGAAACGTTAGATGTTATTAAATTTGCACAAAAAAACAATATTAATACTATCATTTCGCATCGCTCTGGCGAAACTGAAGACACTTTTATTGCAGATTTAGCGATTGGTGTAGGTGCTGGGCAAATTAAAACAGGTTCATTATCACGTTCTGAACGGATAGCGAAGTATAATCGTATTTTAGAAATCGAACAAGAATTAAAGGATAAATTAATTTATGAACCAAGTAAATTTTTTAAATTTAGATAA", "aa": "MVGDDLYCTNLKYLQKGIDFKATNAILIKPNQIGTISETLDVIKFAQKNNINTIISHRSGETEDTFIADLAIGVGAGQIKTGSLSRSERIAKYNRILEIEQELKDKLIYEPSKFFKFR", "aa_hexdigest": "5c090ee2e7eb53d2794490c217aa003b", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2E9FKC7", "query_cov": 0.8389830508474576, "subject_cov": 0.8181818181818182, "identity": 0.6970000000000001, "score": 142.0, "evalue": 1.62e-41, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A2E9FKC7" }, "pscc": { "uniref50_id": "UniRef50_A0A2E9FKC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E9FKC7" ], "product": "Enolase" }, "genes": [], "id": "GPKLONFDEH_395", "locus": "GCA000006625_00362" }, { "type": "cds", "sequence": "contig_1", "start": 223081, "stop": 223638, "strand": "+", "frame": 1, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005945", "GO:0006002", "GO:0016208", "GO:0030388", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0070095", "SO:0001217", "UniRef:UniRef50_Q9PQV8", "UniRef:UniRef90_Q9PQV8" ], "nt": "ATGAACCAAGTAAATTTTTTAAATTTAGATAAAAATATTTTAATTATTACTTCAGGTGGTGATGCACCGGGTATGAATGCTTCATTAATTTCATTAATCCATAGATTAATGAATAATAATTTTAATGTTTTTATAGGAATTGAAGGATTATTAGGACTGTACAATAATTTAATTGAACCAATTAAAGATAAACGTGTTTTTGATGTTTATTTTAACGAGCAAGGAACAGTCATTAAGACAAGTAGATTTATTAAATTAGATATTAATGATAAAAAAACACAAATTATTAAAGATAATTTATTAAGTCATAATATTCAAAAAATTATTATCTTAGGCGGCCAAGGTAGTATGCAAGCTGGTTTAGTGTTAACAAAAATGGGCTTTGAAGTCTTTGGAATATTACACACTATTGATAATGATTTTAGTGAAACACAAATGTGTATAGGGGCCTTAAGTGCTGCTTCTTTTAATCAAAAATTATTAAAGTGCCTAAATTACACAGCAAAAGCTCATTGTGCTTTTAATTTAGTTGAGCTTATGGGTCGTGAATGTTCATGA", "aa": "MNQVNFLNLDKNILIITSGGDAPGMNASLISLIHRLMNNNFNVFIGIEGLLGLYNNLIEPIKDKRVFDVYFNEQGTVIKTSRFIKLDINDKKTQIIKDNLLSHNIQKIIILGGQGSMQAGLVLTKMGFEVFGILHTIDNDFSETQMCIGALSAASFNQKLLKCLNYTAKAHCAFNLVELMGRECS", "aa_hexdigest": "99ad1d3c7279f89ee0143851fa0256b4", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "pfkA" ], "truncated": "3-prime", "pseudogene": { "start": 223081, "stop": 223941, "observations": { "mutation": [ 223636, 223804 ], "stop": [ 223636, 223804 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQV8", "query_cov": 1.0, "subject_cov": 0.5763239875389408, "identity": 1.0, "score": 368.0, "evalue": 6.01e-127, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MNQVNFLNLDKNILIITSGGDAPGMNASLISLIHRLMNNNFNVFIGIEGLLGLYNNLIEPIKDKRVFDVYFNEQGTVIKTSRFIKLDINDKKTQIIKDNLLSHNIQKIIILGGQGSMQAGLVLTKMGFEVFGILHTIDNDFSETQMCIGALSAASFNQKLLKCLNYTAKAHCAFNLVELMGRECSWLVNNSVGKLKPILMLTNQDNKYTVDEVIDLIKDKINSIKEYDPLIIVQELIYDQKWYELLVKTFEQKLHKSLRITILNYLQRGAPVSDFDLQLAKDSANVLVDFIVNQNDIKNTNNMYVVINKFDNKLEVIKFNK" }, "paralog": false, "identity": 0.993006993006993, "score": 555.0, "evalue": 3.19e-203, "description": "Internal stop codon at 223636, 223804. Nonsense mutation around 223636, 223804.", "psc": { "uniref90_id": "UniRef90_Q9PQV8", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_Q9PQV8", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005945", "GO:0003872", "GO:0016208", "GO:0005524", "GO:0070095", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0030388", "GO:0006002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQV8", "EC:2.7.1.11", "UniRef:UniRef50_Q9PQV8", "COG:COG0205", "COG:G", "GO:0005945", "GO:0003872", "GO:0016208", "GO:0005524", "GO:0070095", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0030388", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV8" ], "product": "Probable ATP-dependent 6-phosphofructokinase" } }, "id": "GPKLONFDEH_396", "locus": "GCA000006625_00363" }, { "type": "cds", "sequence": "contig_1", "start": 223678, "stop": 223806, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACTAATCAAGATAATAAATATACTGTTGATGAAGTAATAGATTTAATTAAAGATAAAATAAACTCAATAAAAGAATACGATCCTTTAATCATTGTTCAAGAATTAATCTATGATCAAAAATGA", "aa": "MLTNQDNKYTVDEVIDLIKDKINSIKEYDPLIIVQELIYDQK", "aa_hexdigest": "4bbe38bcb1985267934f0350e51b8ec4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5012.681100000001, "isoelectric_point": 4.312624549865723 }, "id": "GPKLONFDEH_397", "locus": "GCA000006625_00364" }, { "type": "cds", "sequence": "contig_1", "start": 224058, "stop": 225482, "strand": "+", "frame": 3, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955", "KEGG:K00873", "RefSeq:WP_006689159.1", "SO:0001217", "UniParc:UPI00000C1BBC", "UniRef:UniRef100_Q9PQV7", "UniRef:UniRef50_Q9PQV7", "UniRef:UniRef90_Q9PQV7" ], "nt": "ATGTTTTCAAAAACACGCATTATTGCTACAGTAGGTCCAGCAACAACAAAATTGTTTTCTAATTGGTTAGAATTTAACGATTTAAATAATATAAATGATGCTCAACAAGTATGCATAAACATTAAAGATTTAATTTTAAGTGGTGTTAATATTTTTCGTATGAATTTAAGTCATGGCGACCAAAAAATACACCTTTTTCGTACACAATTAATTAAAAAAATTGCGGATGAATTAAAGATCAAAGTTGAAATATTATTTGATACAAAAGGACCAGAAATTCGTGTTTGTGAAATGAGTGATAATAATTTTATTATTAAAAATAGTGAAGTTATAATACATTGTAAAGAAAAAGTTTTAGGTAGTTTTAATGAGTTTAGTGTTACTGATGCTACTGGACAATACAATATGATTAGTGATGTTAAAATTAATCACCGAATTTTAATCGACGATGGAAAATTAATTTTAATTGTTAAAAAAATTGATTTTCTAAAAAACATCATTTATACAACCGCTAAAAATAGTTATTCATTAAAAACAAATAAACGCTTAAATTTACCAGATGCCAATTATAGTTTGCCGTTTTTGTCAAAGAAAGATATTGATGATATAAATTTAGCAGTTAAATTAAAAATTCCTTATTTGGCTTTATCGTTTATTTCTAATATTAAACAAATTAATGAGGTTAAACAACTTTTAAAAAAGTCCTCATTTATACCAAAATTAATTGCAAAAATTGAAACTCAAGAAGCAATTGATAATCTAGAAGAAATTATTAAAAATACTGATGGTATCATGGTTGCGCGTGGGGATTTAGGGCTAGAAGTACCTTTTTATAAAATTCCTATTTATCAAAATAAAATTGTTGAATTATGCCATAAATACAATAAATATTGTATTATAGCGACACAAATGTTAGATTCATTAGAACGTAATATTATCCCCACACGAGCTGAAGTCACAGATGTTTATTATGCCGTTAAACAAAAGGTTAACGCAACAATGTTATCAGGCGAAACGGCCGCAGGAATTGATCCAATTAATGCTGTACAAGTTATGAAATCTATTATTTTAGAGACAGAAAATAATAATATAGAAAACGAAATAACATTTAAAAAAAATGAATTATTAATTCCTAAGGAAGTTAAATTAAAAATTTTATCATTTCATAAATTAAAAATAAATAAACATAAATTATTAGTTGTTAATTTAATTAGTAAAAATTCTAAACATTTAGTTTATGATATATTAAAATATGATTTTAATTTTAATATTTTATTTGTTATAGATAAAAATCAAGAACATGATTTATTATTAAAAAATGCTAATTTTATTATTAGTGAAAATAAAATGTTAAAACAAGAAGAATTATTAAAATCATTTAATATTGATTTTAATAATCATGAATTTTTATTTATTTCAATTTAA", "aa": "MFSKTRIIATVGPATTKLFSNWLEFNDLNNINDAQQVCINIKDLILSGVNIFRMNLSHGDQKIHLFRTQLIKKIADELKIKVEILFDTKGPEIRVCEMSDNNFIIKNSEVIIHCKEKVLGSFNEFSVTDATGQYNMISDVKINHRILIDDGKLILIVKKIDFLKNIIYTTAKNSYSLKTNKRLNLPDANYSLPFLSKKDIDDINLAVKLKIPYLALSFISNIKQINEVKQLLKKSSFIPKLIAKIETQEAIDNLEEIIKNTDGIMVARGDLGLEVPFYKIPIYQNKIVELCHKYNKYCIIATQMLDSLERNIIPTRAEVTDVYYAVKQKVNATMLSGETAAGIDPINAVQVMKSIILETENNNIENEITFKKNELLIPKEVKLKILSFHKLKINKHKLLVVNLISKNSKHLVYDILKYDFNFNILFVIDKNQEHDLLLKNANFIISENKMLKQEELLKSFNIDFNNHEFLFISI", "aa_hexdigest": "6f5a70da9a51119ed1b32bb923540790", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BBC", "ncbi_nrp_id": "WP_006689159.1", "uniref100_id": "UniRef100_Q9PQV7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BBC", "RefSeq:WP_006689159.1", "UniRef:UniRef100_Q9PQV7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQV7", "uniref90_id": "UniRef90_Q9PQV7" }, "psc": { "uniref90_id": "UniRef90_Q9PQV7", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_Q9PQV7", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV7" ], "product": "Pyruvate kinase" }, "genes": [ "pyk" ], "id": "GPKLONFDEH_398", "locus": "GCA000006625_00365" }, { "type": "cds", "sequence": "contig_1", "start": 225605, "stop": 226981, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATAATAAAAATTATACTGAGAAATTTATTACTGATAAAGTAATGCGAAGAGATTATTCAAAAATTAAATCTAATTTTGAAGGACCAAATCTACTTGAAATTCAAGTTGAATCTTTTAAACGCTTTATGGAGAAAGATTTAAAAGAAGTTATTTCAAGTATTTTTCCATTAAAATCTCCTCAGGGTAAGTATACGTTAGCATTTAAAGGTTTAAAAATTAAGCAGCCAACAAAAGATGAAAGTGCTTGTCGTGACGAAGGTAAAACATTTGAAACACCAATTTATATTGATTTGGAATTAACAGATAACTACACAGGTGAAGTTAAACGTGCACAACGAAATACTAAAACAGGAGAAGATGGTATTTATTTAGGAGCAATTCCCAAAATGACAGAAAAAGGAACTTTTGTTATTAATGGAATTGAAAAATTTGTTATCTCACAAATTGTTAGATCACCAGGAATTTATGTACTTGGTAAATCAAGCATCAAATTGAACGGTTCACGTAAGCGTTTATTTGAAGGCAAAATTTGTGAAATTTATCCTTCTAAGGGTACTTTGATGCTTGGTTATATTCCTAAGGATCGTCATAATATTCAAATTGTTGCACGTGATTCATCTGGTGATAATGCGCAAACATTTTCAGTAACTACTTTACTAAAAGCTTTTGGTTTAACAAGTGCTGAAATCTTAAAAATTTTTAATAATGAAAAAGAGATTCGTGAATCATTAGAAATTGAAAAATATGCGCCAGAATATATTTTTGAAAATTCACAAGAAAATGAAATTATTTTTAAAATTTATAGTGATGCTCATGATATTCATGAGAAAGCCGATCGTCGAGAATCAAATAATAAGTTAAAAGAAGAATATATAGAACAAGGTTCACCACTTTTATCAAAGTTAAAACAACTAATTTTTAATTATGTTGAAAAAAACGATGAAATTGATGCATTATTACATGAAAATAAAGATGTTGAAGATGCTAGTTTCATTAAGAATAATAAAAAATTATATGATGAAAGAGAAGAAATCATTAATTGCATCATTAGTGAAAAAGCAGCTAAGGATATCGTTGAATTATTAGGTATTAATATTAAAAATATAGAAACACTAAGACATTTAGGCAAAGCTTCTTATCAAATAGCTTTACAACAACATTTTTTTAATAAGCGTTTATATGATATTAGTAGTGCTGGACGCTATAAATTTGAAAAAAAATTATTATTAAGTGAACGTTTATATCAAAAAGTAATTGCGAATGATATTATTGATAAAAAAAATAATATTCTAATTCCTAAAGATACTTTAATTACTAAAGAACATATCGAACTAATAAAAAAAGAATCACGTGATAAAAACATTAAATGA", "aa": "MQNNKNYTEKFITDKVMRRDYSKIKSNFEGPNLLEIQVESFKRFMEKDLKEVISSIFPLKSPQGKYTLAFKGLKIKQPTKDESACRDEGKTFETPIYIDLELTDNYTGEVKRAQRNTKTGEDGIYLGAIPKMTEKGTFVINGIEKFVISQIVRSPGIYVLGKSSIKLNGSRKRLFEGKICEIYPSKGTLMLGYIPKDRHNIQIVARDSSGDNAQTFSVTTLLKAFGLTSAEILKIFNNEKEIRESLEIEKYAPEYIFENSQENEIIFKIYSDAHDIHEKADRRESNNKLKEEYIEQGSPLLSKLKQLIFNYVEKNDEIDALLHENKDVEDASFIKNNKKLYDEREEIINCIISEKAAKDIVELLGINIKNIETLRHLGKASYQIALQQHFFNKRLYDISSAGRYKFEKKLLLSERLYQKVIANDIIDKKNNILIPKDTLITKEHIELIKKESRDKNIK", "aa_hexdigest": "b26cf638b776eee893d209f2d2e5971b", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 53023.19719999994, "isoelectric_point": 8.823997306823731 }, "id": "GPKLONFDEH_399", "locus": "GCA000006625_00366" }, { "type": "cds", "sequence": "contig_1", "start": 227138, "stop": 228370, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04561.19", "PFAM:PF04565.21", "PFAM:PF10385.14", "PFAM:PF00562.33" ], "nt": "GTGGCTGACTTAGTTGCTACAACAAGTTATATTTATAACTTGAATTATGAAATTGGTGAATTTGATGATATAGATCATTTAGGTAATAAAAGACTTAAATTGATTCATGAATTATTACGAGCAAGAATTGCTACAAGTATGGCTCGTATTGAGAAGTTTATTAATGAAAAGTTGGCTATTTCTGATGGTTCAAGCAATAACATTACAAATGTTAATGATAAAGGTATTGATACTGAACTTGATCGTGAAATTGAAGAAAGCGATATGAGTGATGAGGAAAAGAAAAAAGCAATTAGTGTTAAATCAATTATTAATACAAAACAATTCCAAAGTTTAGTTAAAGATTTTTTTAATTCACATCAATTAATTCAATTTATTGACCAACAAAATCCATTAGCAGAATTAACTAATAAACGTCGTATTTCAGCGATGGGTCCAGGAGGAATTAGTCGTGAAGACCCTAATTTAGATATTCGTGATGTTCATCACTCTCACTCTTCGCGTATTTGCCCGATTGAAACACCAGAAGGAATGAATATTGGGTTGATTATGTCTTTAGCATCACTTGCAAAAGTAGATGAAAATGGTTTTATTGTAGCACCTTATTATGTTGTTGAGGATGGGGTTGTAAAAGAAGATTATAAATATTTAACAGCCCATGAAGATGATAACTATATTATTGCTGAATCATCAGTCCAATTAGATGAAAATAAACGAATTTTAGATGAACAAGTTGTTGCTCGATATCGTGGCTCAACAGGTTTATTTAGTCCTAATGAAGTTGATTTTATTGATATTGTTCCAAAACAAGTAGTATCAATTGCTGCATCAGCAATTCCATTTATTGAAAATGACGATGGGGCACGTGCTTTGATGGGGTCAAACATGCAACGTCAAGCAACACCTTTAATTAAACCATATGCCCCTATTGTAGGGACAGGTACAGAATTTAAAATTGCACACGATTCAGGAATGGCTGTTGTTGCTAAAAATGACGGAGTGGTTGAATTTGTTGATAGTCAAAAAATTATCATTAGAAATGATAATGATAAATTAGATGATTATAAATTAATTAAATATCGTAAATCAAATCAAGACACATGTAATAACCAAATTCCAATTGTAAAAGTAGGTCAAAGAGTTCACAAATCTGAAACAATTGGTGATGGACCTGCAATGCAAAATGGTGAATTAGCTTTAGGTCGTAATATTCTAGTTGGTTATACAACTTGA", "aa": "MADLVATTSYIYNLNYEIGEFDDIDHLGNKRLKLIHELLRARIATSMARIEKFINEKLAISDGSSNNITNVNDKGIDTELDREIEESDMSDEEKKKAISVKSIINTKQFQSLVKDFFNSHQLIQFIDQQNPLAELTNKRRISAMGPGGISREDPNLDIRDVHHSHSSRICPIETPEGMNIGLIMSLASLAKVDENGFIVAPYYVVEDGVVKEDYKYLTAHEDDNYIIAESSVQLDENKRILDEQVVARYRGSTGLFSPNEVDFIDIVPKQVVSIAASAIPFIENDDGARALMGSNMQRQATPLIKPYAPIVGTGTEFKIAHDSGMAVVAKNDGVVEFVDSQKIIIRNDNDKLDDYKLIKYRKSNQDTCNNQIPIVKVGQRVHKSETIGDGPAMQNGELALGRNILVGYTT", "aa_hexdigest": "adc1c5c34a53e61601d767229b7deb41", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04561.19", "name": "RNA_pol_Rpb2_2", "length": 31, "aa_cov": 0.07560975609756097, "hmm_cov": 1.0, "evalue": 5.34000907097619e-07, "score": 25.05816078186035, "start": 3, "stop": 33 }, { "id": "PF04565.21", "name": "RNA_pol_Rpb2_3", "length": 69, "aa_cov": 0.16829268292682928, "hmm_cov": 0.9855072463768116, "evalue": 7.759375132626135e-26, "score": 85.70551300048828, "start": 124, "stop": 192 }, { "id": "PF10385.14", "name": "RNA_pol_Rpb2_45", "length": 65, "aa_cov": 0.15853658536585366, "hmm_cov": 1.0, "evalue": 5.225890468718725e-22, "score": 73.31855010986328, "start": 202, "stop": 266 }, { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 87, "aa_cov": 0.2, "hmm_cov": 0.8850574712643678, "evalue": 1.9488701973325114e-12, "score": 42.503929138183594, "start": 328, "stop": 409 } ], "seq_stats": { "molecular_weight": 45733.08760000003, "isoelectric_point": 5.043517112731934 }, "id": "GPKLONFDEH_400", "locus": "GCA000006625_00367" }, { "type": "cds", "sequence": "contig_1", "start": 228470, "stop": 229540, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "ATGAAAACAAAAAACGGTGATGAAGAAATCACTCGTGACATGCCTAATGTCTCTGATACTGCAAAACGTTTTTTAGATAATCAAGGTATCGTTTTAGTTGGCGCTGAAGTTCATGAAGGAGATGTTTTAGTTGGAAAAACAACTCCACGAGGTAATGTTGAAACTGCTCCAGAAGATCGTTTATTGCAAACAATTTTTGGCGATAAATCAAAAACAGTTAAAGATTCATCACTAAAAGTAAAACACGGTCAAGAAGGAATTGTTGCTGCTGTTAAGCGAATTAAATCAAGTGATGAAAATGGTAGTGAATTACCAGATGACGTAATTGAAATTATCAAAGTTTATATTGTTCAAAAACGTAAAATTCAAGTTGGTGACAAAATGGCAGGGCGTCATGGAAATAAAGGAATTGTATCAAAAGTTGTTCCTATTCAAGATATGCCTTTTTTAAAAGACGGGACTCCATTAGATATTATGTTAAATCCATTAGGTGTACCTAGTCGTATGAATATTGGTCAAATTTTAGAATTGCACTTAGGTTATGCTGCGGCTGAAATAGGTAAAAAACAATTAATTCAAATTGCAATTGATCAATTAGGTTATGAAAAATATATTTCATTGTTTGGAATCAATGAAATTATTGCTAAAAAACTTTATGAAAATATTAGTAATTTAATTAAACACAAACAAGCAAAACAGGCAAAAGATATAGATTTAATTGATGTGACAATTATTCTAAAAGAATTGGGATTATCATATGATGATATTGGAATTAAAATTTCAACTCCTGTATTTGATGGTGCAAATCATGATGATATTGTTTCTATTATGAATGAAGCAAACATTGATATTGAAAATAATAAGGGTAAACAAGTTCTATATGATGGAAGAACTGGTGAACCATTCGATGGTTTAATTTCAGTTGGATTAACATACATGTTAAAACTAGATCATATGGTTGATGATAAAATTCACAGTCGTTCGGTTGGTCCATATAGTAAAATTACACAACAACCATTGGGTGGTAAATCACAAAATGGAGGCCAACGTTTTGGTGAAATGGAAGTTTGA", "aa": "MKTKNGDEEITRDMPNVSDTAKRFLDNQGIVLVGAEVHEGDVLVGKTTPRGNVETAPEDRLLQTIFGDKSKTVKDSSLKVKHGQEGIVAAVKRIKSSDENGSELPDDVIEIIKVYIVQKRKIQVGDKMAGRHGNKGIVSKVVPIQDMPFLKDGTPLDIMLNPLGVPSRMNIGQILELHLGYAAAEIGKKQLIQIAIDQLGYEKYISLFGINEIIAKKLYENISNLIKHKQAKQAKDIDLIDVTIILKELGLSYDDIGIKISTPVFDGANHDDIVSIMNEANIDIENNKGKQVLYDGRTGEPFDGLISVGLTYMLKLDHMVDDKIHSRSVGPYSKITQQPLGGKSQNGGQRFGEMEV", "aa_hexdigest": "69fbb94408893c010217735acf4a718a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 347, "aa_cov": 0.9662921348314607, "hmm_cov": 0.7550432276657061, "evalue": 1.3602919640499677e-97, "score": 322.7413024902344, "start": 2, "stop": 345 } ], "seq_stats": { "molecular_weight": 39178.53360000003, "isoelectric_point": 5.96033000946045 }, "id": "GPKLONFDEH_401", "locus": "GCA000006625_00368" }, { "type": "cds", "sequence": "contig_1", "start": 229667, "stop": 229909, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGAATCATTTAAATTATTAACAAAACAAATGCAAGGTTTAGGGTTATGTATTACCGTTGAAACAAAAGATGATCGTATGGTAGATATTAATGAATATACACTAAATCAAAATCGTTTAAATAATGACGATGATGAGGTTATTTTAGATGAAAATCTAAAAGAGATCAATGATTCTAATGAAGAAATATTTAATACAAACTTTAATAATAATGACTATGATGATGAAGAGAACTTCTAA", "aa": "MPESFKLLTKQMQGLGLCITVETKDDRMVDINEYTLNQNRLNNDDDEVILDENLKEINDSNEEIFNTNFNNNDYDDEENF", "aa_hexdigest": "49a511866c6a46715b33956c357144ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9453.107499999995, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_402", "locus": "GCA000006625_00369" }, { "type": "cds", "sequence": "contig_1", "start": 230063, "stop": 230242, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EDE8919", "PFAM:PF04997.17" ], "nt": "TTGTTTGATGAAAGTATTTTTGGGCCTTCAAAAGATTATGAATGTTATTGTGGAAAATATCGTAAAGTAAAACATAAAGGTAAAATTTGTGAACGTTGTCATGTTGAAATTACTGAATCAATTGTACGTCGTGAACGAATGGGACATATTGAGTTAGCTGCTCCTGTTGCCCATATTTGA", "aa": "MFDESIFGPSKDYECYCGKYRKVKHKGKICERCHVEITESIVRRERMGHIELAAPVAHI", "aa_hexdigest": "903f56e9f727fcfd1b7191ab57fbb5af", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001EDE8919", "query_cov": 0.9830508474576272, "subject_cov": 0.8055555555555556, "identity": 0.7240000000000001, "score": 95.1, "evalue": 1.39e-24, "valid": false, "uniref50_id": "UniRef50_UPI001EDE8919" }, "pscc": { "uniref50_id": "UniRef50_UPI001EDE8919", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EDE8919" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 60, "aa_cov": 1.0, "hmm_cov": 0.6833333333333333, "evalue": 3.900468219096405e-12, "score": 41.686378479003906, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 6895.970699999998, "isoelectric_point": 8.413913917541507 }, "id": "GPKLONFDEH_403", "locus": "GCA000006625_00370" }, { "type": "cds", "sequence": "contig_1", "start": 230555, "stop": 230782, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAGATTCTAATATTCCTTTTTCAGTGAAAGATGTAATGGCTTTTATTGAAAAACATACAGGCGTTCGTTTTGGTATTGGTGCTGAAGCTATTCGTGAATTATTAGAAAAACTAAATCTTGAAGAAGAACATGAAAAGATTAAACAAGCAATTCAAAATTCACCTAATGCTTATGATCAAAAAACAAAACGCTTATTGCGTCGTTTAGAATGTGTAAGATGA", "aa": "MLKDSNIPFSVKDVMAFIEKHTGVRFGIGAEAIRELLEKLNLEEEHEKIKQAIQNSPNAYDQKTKRLLRRLECVR", "aa_hexdigest": "902af672a3149d54c75af49d2c7d4e62", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8738.046099999992, "isoelectric_point": 8.851718711853028 }, "id": "GPKLONFDEH_404", "locus": "GCA000006625_00371" }, { "type": "cds", "sequence": "contig_1", "start": 230813, "stop": 231358, "strand": "+", "frame": 2, "gene": null, "product": "DNA-directed RNA polymerase subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920VHC6" ], "nt": "ATGGTAATGACAAGAATCCCTGTCACACCATCTGAAACAAGACCAATTATTTCTTTAGATGGCGGTCGTTTTACGACCTCTGACACAAATAATTTTTATCGTAAAATCATTATTCGTAATGAACGTTTAAAACAAATGCAAGCAACAGATGCTCCTGAAATTTTATTAGATAATGAAAAACGTTTATTACAAGAAGCAGTTGATTCATTATTTGATAATAATTCACGTAAAAAACCAGTTGTAGGTAAAGATAAACGTCCTTTAAAATCATTGTCAAATCATCTTAAAGGAAAACAAGGATTATTTAGACAAAATTTATTAGGAAAACGTGTTGACTATTCAGGTCGTTCTGTAATTGTTGTTGGTCCTGAATTAAAAATGTATGAAGTAGGAATTCCTGCTTTAATGATTTTAAAATTATTTAGACCATATATTATTTCTGAATTAATACGTAAACGTGATGAATTAGGTAATGAAATTCAACCAATTTGTGCCAATATTAAATTAGCAGAACAAAAAATTCTTGCGCAAGATAATGAGATTTGA", "aa": "MVMTRIPVTPSETRPIISLDGGRFTTSDTNNFYRKIIIRNERLKQMQATDAPEILLDNEKRLLQEAVDSLFDNNSRKKPVVGKDKRPLKSLSNHLKGKQGLFRQNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFRPYIISELIRKRDELGNEIQPICANIKLAEQKILAQDNEI", "aa_hexdigest": "6b086cf32e129c28d10eaf823f1216c2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920VHC6", "query_cov": 0.8397790055248618, "subject_cov": 0.9440993788819876, "identity": 0.586, "score": 179.0, "evalue": 7.9e-55, "valid": false, "product": "DNA-directed RNA polymerase subunit", "uniref50_id": "UniRef50_A0A920VHC6" }, "pscc": { "uniref50_id": "UniRef50_A0A920VHC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920VHC6" ], "product": "DNA-directed RNA polymerase subunit" }, "genes": [], "id": "GPKLONFDEH_405", "locus": "GCA000006625_00372" }, { "type": "cds", "sequence": "contig_1", "start": 231452, "stop": 231595, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00623.25" ], "nt": "ATGGTGGACGGTAAAGCTATTCGTTTACATCCTCTAGTTACAACCGCTTTTAATGCGGATTTTGACGGAGACCAAATGGCTGTTCATATCCCCTTATCCAAAGAAGCAGTGGCTGAAGCACGATCAATTTTATTAGCCTCATGA", "aa": "MVDGKAIRLHPLVTTAFNADFDGDQMAVHIPLSKEAVAEARSILLAS", "aa_hexdigest": "f0eb94a243cc342762d6e08de14f3eda", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00623.25", "name": "RNA_pol_Rpb1_2", "length": 45, "aa_cov": 0.9574468085106383, "hmm_cov": 1.0, "evalue": 9.967656440329676e-19, "score": 63.76813888549805, "start": 1, "stop": 45 } ], "seq_stats": { "molecular_weight": 5020.739599999999, "isoelectric_point": 5.3670446395874025 }, "id": "GPKLONFDEH_406", "locus": "GCA000006625_00373" }, { "type": "cds", "sequence": "contig_1", "start": 231647, "stop": 232312, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04983.23" ], "nt": "ATGATTTTAGGTATTTATTATTTAACAAAAGAAAAATTTCCACAAGTAATTGAAGAAATGATGGCTAAAGATCCAACGCAAGCGAGAGTTGAATTTATTAATAATTTTCACATTTTTTCAACACAAGACGAAGCTATTCGTGCTTATAAATTAAAAACAATTCGTATTAATGACGTAATTGGAATTACAACAAAAGCTTTTAACAATAAAACATTTAGTAAAGAAGGTATTTTGGTTACAACGGTTGGAAAAATTATCTTCAACCAAGCTTTTCCTGTTAATTTTCCATATATTAATGATGTTAAAAATCTATATGGCGAAAATCAATTTGAAATTATTGGAATGCATGAAAGTATTTTAGATTATTTAAAAGCCTATAATTTAAAAGAACCATTAACTAAAAAAACTTTATCAACAGTAATTGATTATTTATATAAAGTTTCTGAAATCGAAGTCGTACCACAAACAATGGATAAAATTAAAGCTTTAGGATTTAAATATTCAATGATTTCAGCGACAAGCATTTCTGCCTTTGATATTCCATCTTATGATCAAAAATATGAATATTTTAAAGAAACTGATGAATTGGTTTCAAAACTTCGTGAATTCTATTTAGATGGTAAATTAACTGATGATGAGCGATATACTAAAGTTGTTCAAGCTTGA", "aa": "MILGIYYLTKEKFPQVIEEMMAKDPTQARVEFINNFHIFSTQDEAIRAYKLKTIRINDVIGITTKAFNNKTFSKEGILVTTVGKIIFNQAFPVNFPYINDVKNLYGENQFEIIGMHESILDYLKAYNLKEPLTKKTLSTVIDYLYKVSEIEVVPQTMDKIKALGFKYSMISATSISAFDIPSYDQKYEYFKETDELVSKLREFYLDGKLTDDERYTKVVQA", "aa_hexdigest": "4f530d8e5673e3921b1ed8dde912f581", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04983.23", "name": "RNA_pol_Rpb1_3", "length": 183, "aa_cov": 0.8144796380090498, "hmm_cov": 0.7923497267759563, "evalue": 3.3286991014394184e-21, "score": 71.4329833984375, "start": 1, "stop": 180 } ], "seq_stats": { "molecular_weight": 25683.299600000013, "isoelectric_point": 5.8308507919311525 }, "id": "GPKLONFDEH_407", "locus": "GCA000006625_00374" }, { "type": "cds", "sequence": "contig_1", "start": 232394, "stop": 233848, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "ATGGCAAAATCAGGAGCTCGTGGAAACACGTCAAACTTTACACAGTTAGCAGGAATGCGTGGTTTAATGTCAAAATCATATAACTATGACCAAAAAAATAATAATGGTGTTATTAAAGATACAATTGAAATTCCAATTAAGCATTCATTTATTGAAGGATTATCAGTTAGTGAATACTTTAATTCATCATTTGGAGCACGTAAAGGGATGACTGATACGGCGATGAAAACCGCAAAATCAGGTTATATGACACGTAAGTTAGTTGATTCAACACAGGCTGTTGTAATTAAAGATCATGATTGTGGCACAAAAGAAGGTATTATTGTTCGTGAAATTCGAAATACAAAAGATAATACATCAATTGAATCACTAAAAGATCGTATTGTTGGAAGATATAGTATTAATACAATTTATGATACAAAAAATAAATTAATTATCGAAAGTGATAAATTAATTACAAGCGAAATAGCTAATATCATTCAAAATTCAGGTATTCGTGAAGTTGAGGTTCGTTCTCCATTACATTGTGCATCACTTTATGGTGTTTGTCAAAAATGTTTTGGTTTAGATCTATCAACAAATAAATTAATTGAAACCGGTACAGCGATTGGAGTAATTGCAGCGCAATCAATTGGTGAACCTGGAACACAGTTAACAATGCGTACTTTCCATACTGGAGGGGTTGCTGGTGATACGAATATTACTCAAGGGTTTGAAAGAATTAAACAATTATTTGATTGCATTCAACCACAAGAAAATGAGAAAGCAGTTATTTCACAAGTAAAAGGAACTGTTGAACGAATCGAAAAAGATTCAAACACCAATGGTTATAATGTTGTAATTAAATATAATAAAGATAATTATGTAAATTATCCAACACGTTCGAATGCTGTATTACGTGTTAAAACAGGAGATGAAATTATTGCTGGTCAAAAAATTACTGAAGGATCAATTGATGTTAATGATTTACTAAAATATGCAGGTATTGAAAATGTGCGTCATTACATTATTAAAGAAGTTCAAAAAGTTTATCGTATGCAAGGAATTGAGATTTCAGATAAGTATATTGAAGTAATCATTTCACAACTTACAAATAAAATTACAATTACAAATCCAGGAGATTCAGGATTATTTGTTGGAGAAACAATTAGTATTAATGAATTTACTGAAGTGGCTCAAAATATGCTTGTTAATAAGAAAAAACCACCAAGTGCAATTAATCAAGTATTTGGTTTAGACCATGCGCCAAGTAAATCAGGTTCATTCTTATCTGCTGCTTCGTTCCAAGATACTAAGAAAATATTAACTGACGCTGCTGCGCGTTCACAAAAAGATATGTTAATCGGCTTGAAAGAAAACGTTATTCTAGGAAATTTAATTCCTGCTGGAACAGGATTAAAGGATGTTGAAGAAGTGATTGCTTATGGTGAAGAAATGTATAAAAAGCAATACTAA", "aa": "MAKSGARGNTSNFTQLAGMRGLMSKSYNYDQKNNNGVIKDTIEIPIKHSFIEGLSVSEYFNSSFGARKGMTDTAMKTAKSGYMTRKLVDSTQAVVIKDHDCGTKEGIIVREIRNTKDNTSIESLKDRIVGRYSINTIYDTKNKLIIESDKLITSEIANIIQNSGIREVEVRSPLHCASLYGVCQKCFGLDLSTNKLIETGTAIGVIAAQSIGEPGTQLTMRTFHTGGVAGDTNITQGFERIKQLFDCIQPQENEKAVISQVKGTVERIEKDSNTNGYNVVIKYNKDNYVNYPTRSNAVLRVKTGDEIIAGQKITEGSIDVNDLLKYAGIENVRHYIIKEVQKVYRMQGIEISDKYIEVIISQLTNKITITNPGDSGLFVGETISINEFTEVAQNMLVNKKKPPSAINQVFGLDHAPSKSGSFLSAASFQDTKKILTDAAARSQKDMLIGLKENVILGNLIPAGTGLKDVEEVIAYGEEMYKKQY", "aa_hexdigest": "5dc70851e7260d133a88f1a70d91316b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 55, "aa_cov": 0.10537190082644628, "hmm_cov": 1.0, "evalue": 7.729383812415093e-12, "score": 40.71934509277344, "start": 1, "stop": 51 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 340, "aa_cov": 0.6714876033057852, "hmm_cov": 0.7617647058823529, "evalue": 8.421124903761661e-62, "score": 204.57986450195312, "start": 53, "stop": 377 } ], "seq_stats": { "molecular_weight": 53352.126299999974, "isoelectric_point": 8.501010704040528 }, "id": "GPKLONFDEH_408", "locus": "GCA000006625_00375" }, { "type": "cds", "sequence": "contig_1", "start": 233971, "stop": 234492, "strand": "+", "frame": 1, "gene": null, "product": "MBA N-terminal paralog", "db_xrefs": [ "RefSeq:WP_006688938.1", "SO:0001217", "UniParc:UPI00000C1BBD", "UniRef:UniRef100_Q9PQV4", "UniRef:UniRef50_Q9PQV4", "UniRef:UniRef90_Q9PQV4" ], "nt": "ATGAAATTATTAAAAAACAAAAAAATTTGGGTATTAACAATTAGTAGTGTTTTAATTGGTGTAAGTGTTATTACATCAATTGCTTCATGCTCTAGTTCAAACATAGAATCTAAATTAAGTGATAAATTAGTTGAAGATGAAGATAATAATTATTACGTAGCATATCAAATTTCAAATTATGAAAAATTAAAAGATTTTGAAAAAAAACAATTAGAAAAAGTCACTTTTTCAACATCTATTACCCCAACTGATAAAACAAAAAACTCACAAGTTTTTAATGCAAAAGTAAATGAAGGCATTTTATTCAATCAGCAAGCGAAAACATTTTATATCAAATTAGTTCGTAAGCCTGAAGTTGGTGAAAGTATTATTATTTTACCAAATAATTCAATTTTAAAAACACACGGCTTTGTAGTTACAAATGAAAATTTAAATTTTATTAAAATTAATAAAAAAGGCGAATTACAAAGATCTAATACGGCAATAACTAAAAAAAATAATTTAACTAACGAATATAAATAG", "aa": "MKLLKNKKIWVLTISSVLIGVSVITSIASCSSSNIESKLSDKLVEDEDNNYYVAYQISNYEKLKDFEKKQLEKVTFSTSITPTDKTKNSQVFNAKVNEGILFNQQAKTFYIKLVRKPEVGESIIILPNNSILKTHGFVVTNENLNFIKINKKGELQRSNTAITKKNNLTNEYK", "aa_hexdigest": "295636eeac31ee250a8e841de7f7da43", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BBD", "ncbi_nrp_id": "WP_006688938.1", "uniref100_id": "UniRef100_Q9PQV4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BBD", "RefSeq:WP_006688938.1", "UniRef:UniRef100_Q9PQV4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQV4", "uniref90_id": "UniRef90_Q9PQV4" }, "psc": { "uniref90_id": "UniRef90_Q9PQV4", "product": "MBA N-terminal paralog", "uniref50_id": "UniRef50_Q9PQV4" }, "pscc": { "uniref50_id": "UniRef50_Q9PQV4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV4" ], "product": "MBA N-terminal paralog" }, "genes": [], "id": "GPKLONFDEH_409", "locus": "GCA000006625_00376" }, { "type": "cds", "sequence": "contig_1", "start": 234566, "stop": 234826, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAACACCGAATTTATCAACAGCTATAGATAAATTATTAGATATAATTGATAATCAAAATCATTGGCGCACAAATGGTATTAAGAACATTGCAATTCTTACTGATTTAGAAAATGAATCACAAATCAATTCATTAAAAACTTCATTACAAGAAAAAATTCGACAAACTATTGAAATTAAAATTTCATATTTACCGGGTTGTATTTATGCGCATGTGGGTTTAAATAATTTTGCTATCTTAATTGAAGCTAAATAG", "aa": "MDKTPNLSTAIDKLLDIIDNQNHWRTNGIKNIAILTDLENESQINSLKTSLQEKIRQTIEIKISYLPGCIYAHVGLNNFAILIEAK", "aa_hexdigest": "ca29fd1cfaa15a09bc64f31fc6cf3529", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9734.08469999999, "isoelectric_point": 6.045304298400879 }, "id": "GPKLONFDEH_410", "locus": "GCA000006625_00377" }, { "type": "cds", "sequence": "contig_1", "start": 235315, "stop": 235422, "strand": "-", "frame": 1, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV2", "UniRef:UniRef90_Q9PQV2" ], "nt": "ATGAAAACAGCAATTTTAATCGATACAGGTGCTAACGTCTTAGATATTAAAGATGATAATGTATATTTTGTGCCTATTCAGTTAATTATTAGAGAACAACAAATATGA", "aa": "MKTAILIDTGANVLDIKDDNVYFVPIQLIIREQQI", "aa_hexdigest": "d95aae7966d4976729af8440af03af96", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 235285, "stop": 235422, "observations": { "mutation": [ 235293, 235317 ], "stop": [ 235293, 235317 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQV2", "query_cov": 1.0, "subject_cov": 0.7777777777777778, "identity": 1.0, "score": 68.9, "evalue": 6.08e-15, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MKTAILIDTGANVLDIKDDNVYFVPIQLIIREQQIWKRIKRCYWR" }, "paralog": false, "identity": 0.9555555555555556, "score": 85.1, "evalue": 3.2e-24, "description": "Internal stop codon at 235293, 235317. Nonsense mutation around 235293, 235317.", "psc": { "uniref90_id": "UniRef90_Q9PQV2", "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PQV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQV2", "UniRef:UniRef50_Q9PQV2" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV2" ], "product": "Unique hypothetical" } }, "id": "GPKLONFDEH_411", "locus": "GCA000006625_00378" }, { "type": "cds", "sequence": "contig_1", "start": 235567, "stop": 236160, "strand": "+", "frame": 1, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "db_xrefs": [ "COG:COG0073", "COG:J", "RefSeq:WP_006688899.1", "SO:0001217", "UniParc:UPI00000C1BBF", "UniRef:UniRef100_A0AAC9T070", "UniRef:UniRef50_A0A084EZ87", "UniRef:UniRef90_A0AAC9T070" ], "nt": "ATGTATATTTATTATAATAAAACAAGTCTAAACGATACGTTAATTTTTTTAAAAAACAATCAGCAATATGATCATCAAGTGTTTTTAAACGATGACTTATTGCTTTTTTATCATGGTGATGAACTAATTGGTTTAAATTTATTTAATGCAAGTGAATATTTAAAAAATTTAAATGAAGGATATTTATATCCTACGACTCAATTAATTCAAAAATTAAGTGATTTAATAAAAATAAAATTATCATTAGATAATGCTTTTAAAGGTTTTGTGGTAGGTAAAATTTTAGAAGTTAATTTAATTCCCAATACTCATTTACATATTTGCTTAGTAGATATAGGTGATCAACAAATACAAATAATATGTGGAGCACAAAATGTCCGTATTGGCTTAAAAACGGTTGTAGCGACACCTAATTTATTAATGCCAAATGGAAATGAAATTAAAAAATCCAAATTAATGAACTATGATTCATTTGGAATGTTGTGTTCACAAAAAGAATTAAATATTAATAATTTTAATTCACAAGGAATTGTCGAACTTAATGATGAATATCAGGTTGGTCAATTGTTTACAAAAGTTTATAGTAATTTATAA", "aa": "MYIYYNKTSLNDTLIFLKNNQQYDHQVFLNDDLLLFYHGDELIGLNLFNASEYLKNLNEGYLYPTTQLIQKLSDLIKIKLSLDNAFKGFVVGKILEVNLIPNTHLHICLVDIGDQQIQIICGAQNVRIGLKTVVATPNLLMPNGNEIKKSKLMNYDSFGMLCSQKELNINNFNSQGIVELNDEYQVGQLFTKVYSNL", "aa_hexdigest": "495035146bd966b30dc354ea8f261725", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BBF", "ncbi_nrp_id": "WP_006688899.1", "uniref100_id": "UniRef100_A0AAC9T070", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BBF", "RefSeq:WP_006688899.1", "UniRef:UniRef100_A0AAC9T070" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T070", "uniref90_id": "UniRef90_A0AAC9T070" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T070", "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "uniref50_id": "UniRef50_A0A084EZ87", "cog_id": "COG0073", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A084EZ87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EZ87" ], "product": "Phenylalanine-tRNA ligase" }, "genes": [ "ytpR" ], "id": "GPKLONFDEH_412", "locus": "GCA000006625_00379" }, { "type": "cds", "sequence": "contig_1", "start": 236180, "stop": 236569, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13793.12" ], "nt": "ATGTCAAAAAATGATGATATATTATTATTTAGTCTTTCAAATTCACATCAATTAGCTAATAAAATTGCTAATTTACTAAAAATTGAATTATCACCAATTAGAATTGATAAGTTTGCAGATGGTGAATTAATTGTTGCACCTCAAGTTCCAGTGCGTGGACGTCGAGTTATTATTATTCAATCAACATCTAAACCTGTTAACGATAGTTTAATGGAATTATTAATCGCTATTGACTCAATTAAACGAGCTAGTGCCAAAGCAATTAGTGTTGTAATTCCTTATTATGGATATGCACGTCAAGACCGTAAAGCTAAACCACGTGAACCAATTACAGCACGTTTAGTTGCAAAAATGATTGAGAGTGCGGGCGCAACTAGTGTATTAACTTGA", "aa": "MSKNDDILLFSLSNSHQLANKIANLLKIELSPIRIDKFADGELIVAPQVPVRGRRVIIIQSTSKPVNDSLMELLIAIDSIKRASAKAISVVIPYYGYARQDRKAKPREPITARLVAKMIESAGATSVLT", "aa_hexdigest": "9cbcf9c801550e0af72194deb8d7d997", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0023F45C21", "query_cov": 0.9767441860465116, "subject_cov": 0.8811188811188811, "identity": 0.5710000000000001, "score": 135.0, "evalue": 1.86e-38, "valid": false, "product": "ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_A0A953RCZ7", "kegg_orthology_id": "K00948" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13793.12", "name": "Pribosyltran_N", "length": 116, "aa_cov": 0.8992248062015504, "hmm_cov": 1.0, "evalue": 2.356046745087635e-49, "score": 161.72097778320312, "start": 8, "stop": 123 } ], "seq_stats": { "molecular_weight": 14167.48779999998, "isoelectric_point": 9.923183250427243 }, "id": "GPKLONFDEH_413", "locus": "GCA000006625_00380" }, { "type": "cds", "sequence": "contig_1", "start": 236642, "stop": 237172, "strand": "+", "frame": 2, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "GO:0000287", "GO:0002189", "GO:0004749", "GO:0005524", "GO:0005737", "GO:0006015", "GO:0006164", "GO:0009156", "GO:0016301", "KEGG:K00948", "SO:0001217", "UniRef:UniRef50_Q9PQV0", "UniRef:UniRef90_Q9PQV0" ], "nt": "ATGAAACATTATTTTAGTGCTTATAAAGATTCATCAAATATAACTATTGTTTCACCAGATTATGGTGGTGTCAAACGTGCTCGTGAAATTTCAATAGCTACAGGTGCAACATTAGCAATTGTTGATAAACGAAGATCTGGCAAAAATCAGGTGGAAATTAATAATGTATTAGGTGATGTTAAAGATCGTGATTGTGTAATTGTTGATGATATGATTGATACTGGTGGCACAATTTTAGGTGCTGCTAAAATTGTGCGTGAAAAAGGCGCTAAATCAATAACAATTATTGCTACACATGGTTTGTTTAATAATAATGCACGACAACATTTTCAACAAGCAATTAAAGATCGAATTATTAATAAAATTTGTATAGCTGATACAATTGAAAATGAACCTTTTGATGGTCTTGAAATTGTAAGTATCGCTCCAGCAATTGCCAAATGTATTGAAATTTATTCAGAGGGTACAGGTTCGATGTCGTTTGTACATGATGAAAATTCAAAAGTTTTATTTGCTAAAAAAATATATTAA", "aa": "MKHYFSAYKDSSNITIVSPDYGGVKRAREISIATGATLAIVDKRRSGKNQVEINNVLGDVKDRDCVIVDDMIDTGGTILGAAKIVREKGAKSITIIATHGLFNNNARQHFQQAIKDRIINKICIADTIENEPFDGLEIVSIAPAIAKCIEIYSEGTGSMSFVHDENSKVLFAKKIY", "aa_hexdigest": "ff0eff683331a73357ffe838963994bc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "prs" ], "truncated": "both", "pseudogene": { "start": 236342, "stop": 237186, "observations": { "deletion": [ 237161 ], "mutation": [ 236567, 236633 ], "stop": [ 236567, 236633 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQV0", "query_cov": 0.9943181818181818, "subject_cov": 0.5239520958083832, "identity": 0.9940000000000001, "score": 341.0, "evalue": 2.87e-116, "gene_start": 1, "gene_end": 175, "reference_start": 155, "reference_end": 329, "reference_sequence": "MSKNDDILLFSLSNSHQLANKIANLLKIELSPIRIDKFADGELIVAPQVPVRGRRVIIIQSTSKPVNDSLMELLIAIDSIKRASAKAISVVIPYYGYARQDRKAKPREPITARLVAKMIESAGATSVLTWDIHSLQTQGFFDIPFDSLEAVWVLMKHYFSAYKDSSNITIVSPDYGGVKRAREISIATGATLAIVDKRRSGKNQVEINNVLGDVKDRDCVIVDDMIDTGGTILGAAKIVREKGAKSITIIATHGLFNNNARQHFQQAIKDRIINKICIADTIENEPFDGLEIVSIAPAIAKCIEIYSEGTGSMSFVHDENSKVLFAKNILNKNE" }, "paralog": false, "identity": 0.9893238434163701, "score": 524.0, "evalue": 1.1e-190, "description": "Internal stop codon at 236567, 236633. Frameshift due to deletion around 237161. Nonsense mutation around 236567, 236633.", "psc": { "uniref90_id": "UniRef90_Q9PQV0", "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_Q9PQV0", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948", "go_ids": [ "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQV0", "EC:2.7.6.1", "UniRef:UniRef50_Q9PQV0", "COG:COG0462", "COG:F", "KEGG:K00948", "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQV0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQV0" ], "product": "Ribose-phosphate pyrophosphokinase" } }, "id": "GPKLONFDEH_414", "locus": "GCA000006625_00381" }, { "type": "cds", "sequence": "contig_1", "start": 237179, "stop": 237559, "strand": "+", "frame": 2, "gene": "rnmV", "product": "ribonuclease M5", "db_xrefs": [ "COG:COG1658", "COG:J", "EC:3.1.26.8", "GO:0006364", "GO:0043822", "KEGG:K05985", "SO:0001217", "UniRef:UniRef50_Q9PQU9", "UniRef:UniRef90_Q9PQU9" ], "nt": "ATGAATAAACCAATTATCAAAGAAGTTATTATTGTAGAAGGTAAAACAGATGCACAAAAAATTGATCAATTAGTAAATGCACAAATTATTACTACTAATGGATCAGAAATTAATAAAAAGACTTTAGCTTTAATTAAACAAGCACAATTATCAAAAGGAATTATTTTATTTTTAGATCCTGATTATCAAGGAGAAAGAATTCGTAAAATAATTACTAATTATTTAAAAGATGGCATTATTAAACAGTGTTTTATTAGTAAGGACAATATGAAGAATAATTCTAAAAAAATTGGAATTGCTGAAGCTAATAATGATGCTTTATTGAAAGCTTTGAAATCTCAAGGAACATTCGTTATTAATTCAATTGAATCTATTACTTGA", "aa": "MNKPIIKEVIIVEGKTDAQKIDQLVNAQIITTNGSEINKKTLALIKQAQLSKGIILFLDPDYQGERIRKIITNYLKDGIIKQCFISKDNMKNNSKKIGIAEANNDALLKALKSQGTFVINSIESIT", "aa_hexdigest": "26d87bab429e1d78bb34de80c047b42a", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnmV" ], "truncated": "3-prime", "pseudogene": { "start": 237179, "stop": 237718, "observations": { "mutation": [ 237557 ], "stop": [ 237557 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQU9", "query_cov": 1.0, "subject_cov": 0.7039106145251397, "identity": 0.992, "score": 236.0, "evalue": 7.12e-78, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MNKPIIKEVIIVEGKTDAQKIDQLVNAQIITTNGSEINKKTLALIKQAQLSKGIILFLDPDYQGERIRKIITNYLKDGIIKQCFISKDNMKNNSKKIGIAEANNDALLKALKSQGTFVINPIESITWLEYLSLNLNNKKIRLMLCDYLNISYCNHKQLFKRLNMMQKTFKEIKIIIEGF" }, "paralog": false, "identity": 0.9888268156424581, "score": 343.0, "evalue": 9.93e-123, "description": "Internal stop codon at 237557. Nonsense mutation around 237557.", "psc": { "uniref90_id": "UniRef90_Q9PQU9", "gene": "rnmV", "product": "ribonuclease M5", "ec_ids": [ "3.1.26.8" ], "uniref50_id": "UniRef50_Q9PQU9", "cog_id": "COG1658", "cog_category": "J", "kegg_orthology_id": "K05985", "go_ids": [ "GO:0006364", "GO:0043822" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQU9", "EC:3.1.26.8", "UniRef:UniRef50_Q9PQU9", "COG:COG1658", "COG:J", "KEGG:K05985", "GO:0006364", "GO:0043822" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQU9" ], "product": "Ribonuclease M5" } }, "id": "GPKLONFDEH_415", "locus": "GCA000006625_00382" }, { "type": "cds", "sequence": "contig_1", "start": 237722, "stop": 238270, "strand": "+", "frame": 2, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0008649", "GO:0008757", "KEGG:K07056", "SO:0001217", "UniRef:UniRef50_A0A084EZ84", "UniRef:UniRef90_Q9PQU8" ], "nt": "ATGAAATTATGTGATAAAGAATATATTTTATCTGTTATTGCTACACCAATTGGCAATCTTGAAGAAGTTAGTGAACGTGTTATTAAATGTTTAAATGAAGCTTATGTTATTTTATGTGAAGACACTCGAGTTACAGCTAAGCTGTTGCATCTATTAAATATTTATGATTACAAAAAATTAATTAGTTATCATAATTTTAATGAAATTCAAAAATTGAATGAAGCGATTAATTATATTAAAAAATATCCAACAGCTTTAGTTAGTGATGCAGGCTATCCAACAATTTCAGATCCTGGTTATAAATTAATTAATGAATGTCATCAACAAAATATTGGAATTCAAGTTATTAATGGTCCCTCAAGTTTGATGCATGCTCTAGTTGCTAGTGGAATGTGTTCGCAAGATTTTATGTTTTTAGGTTTTTTAGGTAAAACGCAAAAACAACGTGTTGAAAAACTAAAATTATATAAAAACTTGCAAACAACTTTTGTTATTTATGAAGCTGTGCACCGAATAGAAAAGACTTTAATGGATATATATGAAATATGA", "aa": "MKLCDKEYILSVIATPIGNLEEVSERVIKCLNEAYVILCEDTRVTAKLLHLLNIYDYKKLISYHNFNEIQKLNEAINYIKKYPTALVSDAGYPTISDPGYKLINECHQQNIGIQVINGPSSLMHALVASGMCSQDFMFLGFLGKTQKQRVEKLKLYKNLQTTFVIYEAVHRIEKTLMDIYEI", "aa_hexdigest": "e0b2b8e719ec8b854b9a7328b16e7baf", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "rsmI" ], "truncated": "3-prime", "pseudogene": { "start": 237722, "stop": 238561, "observations": { "mutation": [ 238268 ], "stop": [ 238268 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQU8", "query_cov": 1.0, "subject_cov": 0.6523297491039427, "identity": 1.0, "score": 365.0, "evalue": 1.44e-126, "gene_start": 1, "gene_end": 182, "reference_start": 1, "reference_end": 182, "reference_sequence": "MKLCDKEYILSVIATPIGNLEEVSERVIKCLNEAYVILCEDTRVTAKLLHLLNIYDYKKLISYHNFNEIQKLNEAINYIKKYPTALVSDAGYPTISDPGYKLINECHQQNIGIQVINGPSSLMHALVASGMCSQDFMFLGFLGKTQKQRVEKLKLYKNLQTTFVIYEAVHRIEKTLMDIYEIWGDILIFIGRELTKKNESHYYGYLSQLPPITEKGEFVIVIDNKINQDVIKNNNINECLIKIRDLMKQNFKLKDACKEVSKSSNFDSKELYKLIINQK" }, "paralog": false, "identity": 0.996415770609319, "score": 503.0, "evalue": 4.36e-183, "description": "Internal stop codon at 238268. Nonsense mutation around 238268.", "psc": { "uniref90_id": "UniRef90_Q9PQU8", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_A0A084EZ84", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0008649", "GO:0008757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQU8", "EC:2.1.1.198", "UniRef:UniRef50_A0A084EZ84", "COG:COG0313", "COG:J", "KEGG:K07056", "GO:0000451", "GO:0008649", "GO:0008757" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084EZ84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EZ84" ], "product": "Ribosomal RNA small subunit methyltransferase I" } }, "id": "GPKLONFDEH_416", "locus": "GCA000006625_00383" }, { "type": "cds", "sequence": "contig_1", "start": 238460, "stop": 238561, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAAAATTTTAAATTAAAAGATGCTTGTAAAGAAGTTAGTAAATCTTCTAATTTTGATAGTAAAGAATTATATAAATTAATAATTAATCAAAAATAA", "aa": "MKQNFKLKDACKEVSKSSNFDSKELYKLIINQK", "aa_hexdigest": "32f709181d5371fca3b78b43c2138d5b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3906.5285000000013, "isoelectric_point": 9.519095420837402 }, "id": "GPKLONFDEH_417", "locus": "GCA000006625_00384" }, { "type": "cds", "sequence": "contig_1", "start": 238563, "stop": 239789, "strand": "+", "frame": 3, "gene": "rarA", "product": "AAA family ATPase", "db_xrefs": [ "COG:COG2256", "COG:L", "KEGG:K07478", "RefSeq:WP_006688885.1", "SO:0001217", "UniParc:UPI00000C1BC2", "UniRef:UniRef100_A0A2C9DZ19", "UniRef:UniRef50_A0A084EZ83", "UniRef:UniRef90_A0AAP9A9S8" ], "nt": "ATGTTAGATAAATTAATTAAAACTCTAAAAAATCCTCATAGTATTGATGACATTGTTGGTCAAAAATATTTATTAGATGAAATTGGTATAATTAGAAGAATGGTTAATCACCATCAAGTATTTAATTTAATTTTTTATGGCCCCCCAGGTGTAGGAAAATCATCGCTTGCTAAAGTTTTAGCACAAGATTTAAAAGTTCCATTTGCTTTTTTCAATCCAGTTGTTGATTCAAAAAAGCAATTAATGCAAATCATTGAACAAGCATTAGATTTAAATAACTTTATTATCATTATTGATGAAATTCATCGATTAAATAAAGATAAACAAGATATTTTATTACCAATTATTGAAGCAAATAAAATTAAATTATTTGCGACAACAACTGAAAATCCGTTTTTTGTTATTAACCCTGCTTTAAGAAGTCGTTGTCAACTATTATCATTAAAACCATTAACGAGTTCACAAATTAGTGAACGAATTAAAACTTTGATTAATGTTGAAGAAGTTTTTGAACCACAAGCTTTTAATTTATTAATTAATAAAACTAATGGAGATTTAAGGGCTGTTATCAATACATTAGAAATTATTTATTTATTATATAAAGAACATAAAGTTGATTATGTATTACTAAAACGTATTATGCAAGAATCATACGCATTAGGTAGTAGCGATGGCGATGAAATTCATGACTTAAAATCAGCTTTTCATAAATCAATGCGTGGAAGTGATTGTGATGCAGCTTTATATTATTTAGCACGTTTAATAATCATAGGTGATTTTGAAGCGATTTATCGAAGAGTTAGTGTTTGTGTTCATGAAGATATAGGTTTAGCTAATCCTAATCTATGTTTACGTGTTGATCAAGGAATTAATTCTTGTCGATTTTTAGGATATCCAGAAAATCAAGTAATTTTAGCTAATTTAGTTTTACAAATTTGTTTATCAAATAAATCAAACTCTGTATATCTTGCCTATCAAAAAGCATTTGAAGATGCTGCTAATGGCAAACAATATTCAATTCCAAATCATGTCCGTGATGCTCATTATGCATCAGCAATAAAATTGGGTGTTCATGGTTATAAATATCCACACGATTTTAAAAATAACTATGTTAATCAACAATATTTACCACGTGAATTAACAAACAAAAAATATTATGTTCCCCAAAACAACGAACAAGAGTTTAAATTAAACCAAAATCAACAAAAATTTAAAAATAATAATTAA", "aa": "MLDKLIKTLKNPHSIDDIVGQKYLLDEIGIIRRMVNHHQVFNLIFYGPPGVGKSSLAKVLAQDLKVPFAFFNPVVDSKKQLMQIIEQALDLNNFIIIIDEIHRLNKDKQDILLPIIEANKIKLFATTTENPFFVINPALRSRCQLLSLKPLTSSQISERIKTLINVEEVFEPQAFNLLINKTNGDLRAVINTLEIIYLLYKEHKVDYVLLKRIMQESYALGSSDGDEIHDLKSAFHKSMRGSDCDAALYYLARLIIIGDFEAIYRRVSVCVHEDIGLANPNLCLRVDQGINSCRFLGYPENQVILANLVLQICLSNKSNSVYLAYQKAFEDAANGKQYSIPNHVRDAHYASAIKLGVHGYKYPHDFKNNYVNQQYLPRELTNKKYYVPQNNEQEFKLNQNQQKFKNNN", "aa_hexdigest": "53518380aa51a0ae2ce3963bfdca72b5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1BC2", "ncbi_nrp_id": "WP_006688885.1", "uniref100_id": "UniRef100_A0A2C9DZ19", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BC2", "RefSeq:WP_006688885.1", "UniRef:UniRef100_A0A2C9DZ19" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DZ19", "uniref90_id": "UniRef90_A0AAP9A9S8" }, "psc": { "uniref90_id": "UniRef90_A0AAP9A9S8", "gene": "RarA", "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A084EZ83", "cog_id": "COG2256", "cog_category": "L", "kegg_orthology_id": "K07478" }, "pscc": { "uniref50_id": "UniRef50_A0A084EZ83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EZ83" ], "product": "ATPase, AAA family" }, "genes": [ "rarA" ], "id": "GPKLONFDEH_418", "locus": "GCA000006625_00385" }, { "type": "cds", "sequence": "contig_1", "start": 239809, "stop": 240060, "strand": "+", "frame": 1, "gene": null, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "nt": "ATGTTAAAACAAAAGAAATTTTTTATCTCTACTCCCATTTACTATTCTTCTGGAAATCCTCATATTGGTCATGCTTATACAACGATCATTGCTGATGTTTTAGCACGTTATAAACGCTTGTTTGGATATGATGTTTTCTTTTTAACAGGAATGGATGAGCATGGTCAAAAAATCCAACAAAAAGCTTTTGAAGAAAACATTTCTCCCAAAGCTTTGGTCGATCGTAATTCAATTATTTTTTTAAATTTATGA", "aa": "MLKQKKFFISTPIYYSSGNPHIGHAYTTIIADVLARYKRLFGYDVFFLTGMDEHGQKIQQKAFEENISPKALVDRNSIIFLNL", "aa_hexdigest": "61308767792bdd436ce036f9541369a5", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A093YS71", "query_cov": 0.927710843373494, "subject_cov": 0.9871794871794872, "identity": 0.623, "score": 103.0, "evalue": 1.37e-27, "valid": false, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "uniref50_id": "UniRef50_A0A093YS71" }, "pscc": { "uniref50_id": "UniRef50_A0A093YS71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_419", "locus": "GCA000006625_00386" }, { "type": "cds", "sequence": "contig_1", "start": 240106, "stop": 240456, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGGATCATGAAGAGTCTGTACAAAAAGTTTTTAGTTATTTATATAAACAAGGAAAAATTTATTTAGGCCAATGGACAGGTTATTATTGTGTATCATGCGAAGAAAATTATAATCCTGCTGAAATTATTAAAAGTCAAGATAATATAATGTTATGTCGCATGGGTCATAAATTAGAAACTAAGTCTGAAGAATCATATTTTTATAAAATGAGTGATCAAGCACCATTTTTAAAAACTTACTATCAAAATCATCCGAATTTCATTATTCCAAATGAACGTGCAAATGAAATGGTTAATAACTTCTTAAATAATTTAGAAGATTTAAGTATTTCACGTACTACTTTTGATTGA", "aa": "MDHEESVQKVFSYLYKQGKIYLGQWTGYYCVSCEENYNPAEIIKSQDNIMLCRMGHKLETKSEESYFYKMSDQAPFLKTYYQNHPNFIIPNERANEMVNNFLNNLEDLSISRTTFD", "aa_hexdigest": "cf0f62c02569976ad725240e028e08be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 111, "aa_cov": 0.9568965517241379, "hmm_cov": 0.8828828828828829, "evalue": 9.123296157029968e-08, "score": 26.783100128173828, "start": 3, "stop": 113 } ], "seq_stats": { "molecular_weight": 13822.359799999984, "isoelectric_point": 5.149521827697753 }, "id": "GPKLONFDEH_420", "locus": "GCA000006625_00387" }, { "type": "cds", "sequence": "contig_1", "start": 240727, "stop": 241125, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTCTAAATCATTAGGCAATGTAATTGATCCTAATGTTTTAATTGATACTTATGGTGTGGATGCACTTCGTTATTATTTAATGGCTGATTTAAGTTTATTTAGAGATGCTATTTTTAGTGAAGATAATTTGATTGAAACTTATAATACTCAATTAGCAAATAGTTATGGTAACATGATTTCAAGAACATTGGGAATGTTAAAAAAATATCGAAATAATATAGTTCCAAAATATGTTGGTTGTGTTTTAAAAAATGATGAAAAATTAGAAAATTTAATTAATAAAAATATTGAATTAGTTCAAGAAAACATTAATAAATATAGTATTGATAAAGCACTTAATTGCATTCAAGAAATTTTAGTTGAAGCAAATAAATATATTGAAGATAATAAACCTTGA", "aa": "MSKSLGNVIDPNVLIDTYGVDALRYYLMADLSLFRDAIFSEDNLIETYNTQLANSYGNMISRTLGMLKKYRNNIVPKYVGCVLKNDEKLENLINKNIELVQENINKYSIDKALNCIQEILVEANKYIEDNKP", "aa_hexdigest": "8c15c9164e6bc193a5a15b6708c78d11", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 33, "aa_cov": 0.25, "hmm_cov": 0.9696969696969697, "evalue": 2.2704346760558e-09, "score": 32.06669616699219, "start": 1, "stop": 33 } ], "seq_stats": { "molecular_weight": 15160.218599999986, "isoelectric_point": 4.984063529968262 }, "id": "GPKLONFDEH_421", "locus": "GCA000006625_00388" }, { "type": "cds", "sequence": "contig_1", "start": 241462, "stop": 241776, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGTTAAGATATAAAATCTTATGCGCTAGCTTAGGAGTGGTTGGTGTTGTTGGTGTTATTGCTGCAATATCAATTGATTATAAACAAATTCCAATTGTTTATGCAAATGGTAGTTCATCAGTATTACCCTTAATTCAGGCCTTATCTGAAAAACATCATAAACAAGATATTATTTCTAATGCTGGAGGTTCTTCGTTAGGAATAATTGAAGCTGTTGAACAAAGAACTAATATAGGCGTAAGTTCACGTAGTCCAAATATTGATTTTGAGCAACAAAGTGTTGAAAAAAGTCATGTAGTTTATGATAAGTGA", "aa": "MRLRYKILCASLGVVGVVGVIAAISIDYKQIPIVYANGSSSVLPLIQALSEKHHKQDIISNAGGSSLGIIEAVEQRTNIGVSSRSPNIDFEQQSVEKSHVVYDK", "aa_hexdigest": "f9076279e0ff035578fbe8e6290d0773", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11206.731899999995, "isoelectric_point": 7.895588111877441 }, "id": "GPKLONFDEH_422", "locus": "GCA000006625_00389" }, { "type": "cds", "sequence": "contig_1", "start": 241888, "stop": 242145, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAGCTTTTACTGGGAATGAACAAGTAAGTTATTATGATCTTGATCCCCAATTACCAAAAACACCAATTTTAGCTTATTCACGCACAGGTGGAGCAAATGCCTCAGGGACAGCCGAGGCTTTTTTGACTTCTAATCCATTAATTTCTAAAAAAGAATTTAAAAAAAAATATCATGATATTTATAAGGTTATTGAAGCTGGTACTTATGGCAAATTAATAAGAAGTACTGAAGAATCGAATACTCAAGCTTGA", "aa": "MFKAFTGNEQVSYYDLDPQLPKTPILAYSRTGGANASGTAEAFLTSNPLISKKEFKKKYHDIYKVIEAGTYGKLIRSTEESNTQA", "aa_hexdigest": "a95692d4c103bc4f75be903ac5a6c0de", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9441.510999999995, "isoelectric_point": 8.860228538513184 }, "id": "GPKLONFDEH_423", "locus": "GCA000006625_00390" }, { "type": "cds", "sequence": "contig_1", "start": 242494, "stop": 242634, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTAAAACCTTATAAAGATGAACAATCTTTTCAAGAAGCCATTAACAATCAAAGCGCAAAAAACAATTTTTTTGTAAGTGATTTTGAATTAATAGAATCAAATAGAATTAATATATTAGGAGCAAAATCAGCATAA", "aa": "MFLKPYKDEQSFQEAINNQSAKNNFFVSDFELIESNRINILGAKSA", "aa_hexdigest": "dbba823af5d457ef2eed85d72fcc22d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5297.861199999998, "isoelectric_point": 5.052213478088378 }, "id": "GPKLONFDEH_424", "locus": "GCA000006625_00391" }, { "type": "cds", "sequence": "contig_1", "start": 242634, "stop": 242735, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTATTTTAAATCTTAATTCGCAACAGACTAAAAAAATCATTTTTAACAAAAAAATAAAAGATAAACTAGCTAAAACGTTAGTTATTAGTTTTGCTTGA", "aa": "MSILNLNSQQTKKIIFNKKIKDKLAKTLVISFA", "aa_hexdigest": "7d61dad708405164e15fa68a980e06ab", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3777.563500000001, "isoelectric_point": 10.398057365417479 }, "id": "GPKLONFDEH_425", "locus": "GCA000006625_00392" }, { "type": "cds", "sequence": "contig_1", "start": 242895, "stop": 243584, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGTTTCGTTTGGTGCAATTATAATTGCTGCTCCAATTGGTATTAAAACAGCTACTTTTATTAAGTTTAGAATTAAAAACAAAAGATTACAAAAATTTTTTAGAGTCACGATTTTAGCATTAGCTGGAATTCCTTCAGTTATTTTTGGATTATTTGCTATTCAGTCTTTAGGTCCTGCAATTTCGTTTGTTTTCCACATTGATACTGTTCAAAACATTACTACGTCAATGTTTATGTTAGCGTTTATTATTATTCCGACAATTATTTCTTTAACGCTAAGCACATATGATGGTATTGATATGCGTTTAATTGAAAATGGGATAGGAATGGGAAGTTCTTATACACGTTCAATTTATAAGATTTTTAAAAAAGAAGCTCGTGGTGGTATCATTATTGCCATTATTATTGCCTTAGGTCGTGCGATCGGCGAAACAATGGCCATTAGTATGATTTTAAGTGATCAAGGTTACCAAAACATTTTTGGTACTGGTTTTTTACAAATTATGCACTCTGCTCTTCGTCCATTAGGTGCTGTAATTTCAGCTAACATGTTCGCTGAAAATGGTGGCGAAGGTTTAAGAGGATTACTATATATTTATGGGATCGTTTTATTTGTAGCGATTATGATTTTAAATGGCCTTGTAACTTATTTAACAAGAAAACGTTCAAAGAAAACATATGCTTGA", "aa": "MLVSFGAIIIAAPIGIKTATFIKFRIKNKRLQKFFRVTILALAGIPSVIFGLFAIQSLGPAISFVFHIDTVQNITTSMFMLAFIIIPTIISLTLSTYDGIDMRLIENGIGMGSSYTRSIYKIFKKEARGGIIIAIIIALGRAIGETMAISMILSDQGYQNIFGTGFLQIMHSALRPLGAVISANMFAENGGEGLRGLLYIYGIVLFVAIMILNGLVTYLTRKRSKKTYA", "aa_hexdigest": "3def5d03ad42d4fa41c2149739a468e6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25015.7534, "isoelectric_point": 10.216707801818849 }, "id": "GPKLONFDEH_426", "locus": "GCA000006625_00393" }, { "type": "cds", "sequence": "contig_1", "start": 244083, "stop": 244277, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTTTAGTTATTTTTATTGAAATTTTTGGTTTTACTTCAATGGGTAATATTGGTAAATCACTATTAGCGGGTGCTTTAACAATTACTTTAGTAGTCCTTCCAACATTTACACGTTCAATTCAACAATCTTTAAAAGCAGTACCAATGAGCATTCGTGAAAATGCTTATGGATTGGGATGTTCAAAGTGA", "aa": "MFGLVIFIEIFGFTSMGNIGKSLLAGALTITLVVLPTFTRSIQQSLKAVPMSIRENAYGLGCSK", "aa_hexdigest": "ec36543579dc0d99cd07be4f21e8c612", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6853.140700000001, "isoelectric_point": 9.624630165100097 }, "id": "GPKLONFDEH_427", "locus": "GCA000006625_00394" }, { "type": "cds", "sequence": "contig_1", "start": 244374, "stop": 244526, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTTAACAGCAGGTTTATCATCAGCTCAAAGCATAACAATTTTAAATCCTGGACAAACATTAACAACACGTATTTATGCTCAATTATATGAAAATAATACAAATATTGCTCATAATGTAATGTACGAATCTGCTTTTATTACTTTATGA", "aa": "MYLTAGLSSAQSITILNPGQTLTTRIYAQLYENNTNIAHNVMYESAFITL", "aa_hexdigest": "20767dfc4da79e5adee472ed540fc434", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5553.235900000001, "isoelectric_point": 5.380629158020019 }, "id": "GPKLONFDEH_428", "locus": "GCA000006625_00395" }, { "type": "cds", "sequence": "contig_1", "start": 244641, "stop": 244829, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGATATTAAAGTTTTCAAACCACAAATTTATCAAAAACATCTTTATTTAACACATAATCAAGCCAAAATCATGGGATATAATGAATCAGTAAATAAAGTTGCTAAAATTGGTTTAAAGTTATATGAAATTCGTTATGTTGATGATGAACAAATTCAAAAAGAGTTATTAAAAGTTAGGGGATAA", "aa": "MRDIKVFKPQIYQKHLYLTHNQAKIMGYNESVNKVAKIGLKLYEIRYVDDEQIQKELLKVRG", "aa_hexdigest": "041b07d31ae47ce8c2aff510268ed6ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7436.6578, "isoelectric_point": 9.600712394714353 }, "id": "GPKLONFDEH_429", "locus": "GCA000006625_00396" }, { "type": "cds", "sequence": "contig_1", "start": 244834, "stop": 244971, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATCAAAAAGACAATAAAGAAATTCAAACAATTGTTCCTTTAAATCAAAAAACTAATTTAAATGAATTGGATGCTAAAGCAATTTATGATGAATTTCAAAAATTAAATAAATACCAAAGAGCTTTTGCTTGA", "aa": "MNDQKDNKEIQTIVPLNQKTNLNELDAKAIYDEFQKLNKYQRAFA", "aa_hexdigest": "4a971e1008b84b7909edc270e7f42b59", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5342.946800000001, "isoelectric_point": 6.080771827697753 }, "id": "GPKLONFDEH_430", "locus": "GCA000006625_00397" }, { "type": "cds", "sequence": "contig_1", "start": 245104, "stop": 245469, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAATCGAACAAAACATGTTCTACATGATTTAAATTTGGATATTAAAAGAAATAAAGTAACAGCATTTATTGGCCCAAGTGGTTGTGGTAAATCAACTTTTTTAAGAAATTTAAATCAATTAAACGATTTAATAGAAGGGACTAGTCACGAAGGTGAAATTTATTTTTTAGGAACTAATACACGTTCAAAAAAAATATCATCATTAGAATTAAGAACACGAATCGGGATGGTTTTTCAAAAACCAACACCATTTGAAATGAGTATTTTTGATAATGTTGCATATGGTCCACGAAATAATGGAATTAATGATCGTAAAATTTTAGAAAAAATTGTTGAAAAATCATTAAAAAGTGCAGCTTTATGA", "aa": "MNRTKHVLHDLNLDIKRNKVTAFIGPSGCGKSTFLRNLNQLNDLIEGTSHEGEIYFLGTNTRSKKISSLELRTRIGMVFQKPTPFEMSIFDNVAYGPRNNGINDRKILEKIVEKSLKSAAL", "aa_hexdigest": "d46ba8bed11d8ab3330a7fa4ed6f5a9a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_W5SUP8", "query_cov": 0.8347107438016529, "subject_cov": 0.8145161290322581, "identity": 0.5539999999999999, "score": 125.0, "evalue": 6.9e-35, "valid": false, "product": "Phosphate transport ATP-binding protein pstB", "uniref50_id": "UniRef50_A0A3D0XTF9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 104, "aa_cov": 0.8512396694214877, "hmm_cov": 0.8365384615384616, "evalue": 6.754836345870955e-16, "score": 54.73188018798828, "start": 8, "stop": 110 } ], "seq_stats": { "molecular_weight": 13662.59699999998, "isoelectric_point": 9.807913780212402 }, "id": "GPKLONFDEH_431", "locus": "GCA000006625_00398" }, { "type": "cds", "sequence": "contig_1", "start": 245578, "stop": 245736, "strand": "+", "frame": 1, "gene": null, "product": "Phosphate ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9H9", "UniRef:UniRef90_A0A3B0P9H9" ], "nt": "ATGGATGAACCAACAAGTGCTTTAGATCCAATTGCAACAGCTAAAATTGAAGAATTAATATTAGAATTAAAAAAGAAATATTCTATTATTATTGTGACTCACTCAATGGCACAAGCCCAGCGAATAAGTGATGAAACTGTGTTTTTTTATCAAGGGTGA", "aa": "MDEPTSALDPIATAKIEELILELKKKYSIIIVTHSMAQAQRISDETVFFYQG", "aa_hexdigest": "bb12a1e6150c232dc8e11a55bf53d87b", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 245494, "stop": 245823, "observations": { "mutation": [ 245734 ], "stop": [ 245734 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3B0P9H9", "query_cov": 1.0, "subject_cov": 0.46846846846846846, "identity": 0.846, "score": 90.9, "evalue": 1.43e-22, "gene_start": 1, "gene_end": 52, "reference_start": 31, "reference_end": 82, "reference_sequence": "MFQSAHGLSEGQQQRLSIARAIALKPEVLLMDEPTSALDPIATNKIEQLIHQLKKSYSIVIVTHSMAQAQRVSDETVFFYEGRVLEHGTTKQIFTQPNNEKTKDYIDGRIG" }, "paralog": false, "identity": 0.7339449541284404, "score": 159.0, "evalue": 5.12e-52, "description": "Internal stop codon at 245734. Nonsense mutation around 245734.", "psc": { "uniref90_id": "UniRef90_A0A3B0P9H9", "product": "Phosphate ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3B0P9H9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3B0P9H9", "UniRef:UniRef50_A0A3B0P9H9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P9H9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9H9" ], "product": "Phosphate ABC transporter ATP-binding protein" } }, "id": "GPKLONFDEH_432", "locus": "GCA000006625_00399" }, { "type": "cds", "sequence": "contig_1", "start": 245847, "stop": 246059, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACTAATTATTTTTTAATGAAAGAATCACAATGTGATTTACTAAATGATTTTAAAAACTTTTTTCAAATGGTAATTGATAATCAAAAACTAATTTGTGATTTATTAAAAAACGATCAATCATCATATAAAGAAACATATGAAAAAGCTTGTTTTTTAGAAAAAAAAGTTAATACAACATATGCTGATATGTTAGAAGAAATTATGTGA", "aa": "MATNYFLMKESQCDLLNDFKNFFQMVIDNQKLICDLLKNDQSSYKETYEKACFLEKKVNTTYADMLEEIM", "aa_hexdigest": "6c9b76b002d7ada9d9356b4732169967", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8381.5818, "isoelectric_point": 4.580506706237793 }, "id": "GPKLONFDEH_433", "locus": "GCA000006625_00400" }, { "type": "cds", "sequence": "contig_1", "start": 246333, "stop": 246503, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATCAATAAAAATTTAATTCAGTCCTTACATGATCAATCAAAACTATTAGATGAAAACTCAAATTATATTAAATTAATTTTAATGTATCGTCATATTGAACGAAATATTGAACATGGCATAAATATTGTGCAGAACTTTGGTAATGTGCACATTACCAGTCGTTAA", "aa": "MKINKNLIQSLHDQSKLLDENSNYIKLILMYRHIERNIEHGINIVQNFGNVHITSR", "aa_hexdigest": "afe40770043a3e1dbdaf8ea5a0d6beac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6657.597899999999, "isoelectric_point": 9.301514625549316 }, "id": "GPKLONFDEH_434", "locus": "GCA000006625_00401" }, { "type": "cds", "sequence": "contig_1", "start": 246663, "stop": 246887, "strand": "+", "frame": 3, "gene": "copZ", "product": "HMA domain-containing protein", "db_xrefs": [ "COG:COG2608", "COG:P", "RefSeq:WP_006688893.1", "SO:0001217", "UniParc:UPI00000C1BC7", "UniRef:UniRef100_A0A2C9DYU1", "UniRef:UniRef50_A0A2C9DYU1", "UniRef:UniRef90_A0A2C9DYU1" ], "nt": "ATGACTTATATGCTATTAACATCTAGTGACTTACATTGCGGGTCATGTTCTTCAAATTTATATAATGTACTTGAAAAGATTGGCGCACAAAATATTAGTGTAAATATTTTAAATTCAGAATTTGCTTTTGAATTTGAAGAAAATAAAATTGCTGATCAAGATGTAATTAAAGAAATCAATAAAAATGGTTTCAAAACAAATATTTTAGAAAAATACATATACTAA", "aa": "MTYMLLTSSDLHCGSCSSNLYNVLEKIGAQNISVNILNSEFAFEFEENKIADQDVIKEINKNGFKTNILEKYIY", "aa_hexdigest": "a4500e4177adfc807110ce24272f44a9", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1BC7", "ncbi_nrp_id": "WP_006688893.1", "uniref100_id": "UniRef100_A0A2C9DYU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BC7", "RefSeq:WP_006688893.1", "UniRef:UniRef100_A0A2C9DYU1" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYU1", "uniref90_id": "UniRef90_A0A2C9DYU1" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DYU1", "gene": "CopZ", "product": "HMA domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYU1", "cog_id": "COG2608", "cog_category": "P" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYU1" ], "product": "HMA domain-containing protein" }, "genes": [ "copZ" ], "id": "GPKLONFDEH_435", "locus": "GCA000006625_00402" }, { "type": "cds", "sequence": "contig_1", "start": 246891, "stop": 247076, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAGAATAATTTAATTAAAAAATTTCAATTACTACCTATTAATAAAAGAAGATATTTAATTTCTTTAATGAAAACATCAATAGCACTTCTAATTAGTATTCCTTTAATGGTTTTTGAAATGCTATTTATGTTTAAAAGTAATTTAATTTTAAGCATTGATGGTTATTTTATATATGGATGA", "aa": "MLKNNLIKKFQLLPINKRRYLISLMKTSIALLISIPLMVFEMLFMFKSNLILSIDGYFIYG", "aa_hexdigest": "1f2647e6109bb3b268898b2c4d98e724", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7200.871999999996, "isoelectric_point": 10.092283821105958 }, "id": "GPKLONFDEH_436", "locus": "GCA000006625_00403" }, { "type": "cds", "sequence": "contig_1", "start": 247173, "stop": 247742, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTACTTGTTGTTATTTCAACATGTGTTGCTTTCATATATAGTACTTATAGTTTAATTTCTAACACAATTATTTATGAACCTAAACTACATGGTTTTTTTGAAACAGCTTGCATGATTATAGCGACAATGAGTGTTGGTCAATTAGTTAGTGATCGTATTAAACTAAAAGCAAATCAAGATCTACAATCTTTAAATAATTTACAAGTCAAAAAATATAATAGTTATGATTTGAACACAAAACAAGTTAGTGAAAAAGTTGTTTTTCAAGCTAAAATTAACGAATACGCACTAGTTAAAAAAGGTGAAATTGTTCCATTAGATGGCGTATTATATTCACAAATTGCTGAAGTTGATGAATCATCATTAACTGGTGAAGCTCGTCCAATTCTAAAAACAATTAATAATGATATTATTGCTGGATCAATTAATGTTGGAGATAATTTTATTTTTAAAATTACTAAACTTTATAATGATTCAACGATTAAAAAAATTATTAATGGTGTTAATCAAATTGCTAGTAGCAAGCCAAAAATTCAAGTTGTTGCTGATAAGATTTCGTTATGA", "aa": "MSLLVVISTCVAFIYSTYSLISNTIIYEPKLHGFFETACMIIATMSVGQLVSDRIKLKANQDLQSLNNLQVKKYNSYDLNTKQVSEKVVFQAKINEYALVKKGEIVPLDGVLYSQIAEVDESSLTGEARPILKTINNDIIAGSINVGDNFIFKITKLYNDSTIKKIINGVNQIASSKPKIQVVADKISL", "aa_hexdigest": "44d68b5de28b285073c08ed3e54bb108", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20912.074500000017, "isoelectric_point": 8.72890644073486 }, "id": "GPKLONFDEH_437", "locus": "GCA000006625_00404" }, { "type": "cds", "sequence": "contig_1", "start": 247764, "stop": 248813, "strand": "+", "frame": 3, "gene": null, "product": "Copper-transporting P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQU0", "UniRef:UniRef90_Q9PQU0" ], "nt": "ATGGCCATTCTAGCTTTTTTACTACAAGCTTTTGTACCAAGTATTCAAGAGTTACCAATTGCTTTTTTAAATCTTCATGGATCAAATAATGATTCTAATTTATATGAAAAAGCAGCATATGTAGCAGTTTCTGTTCTAGTTATATCATGTCCATGTGCATTTGGTATTGCTGTGCCTTTAGCAGTTTTAATTGGTGCAGGGCATGGTGCTAAAAGTGGGATTACATTTAACAATAGTAATATTTTTGAAAAGATTAAAAAAGTTAATGCAATTGCTTTTGATAAAACAGGGACATTAACTTATGGAAAATTACAATTAAAACAAGTTATAGGAAATGATCAATTTTTAGATTTAATTTATCAAATGGAATTAATTTCTTTACATCCACTAGCAAAATCATTCGTAACTTATGCCATTATTAATAATATCGTCATGAGTACAAAATTAATTGATATTAAAGAAGTTGCAGGAGTGGGGATTATTGCTAAAGATGTTGATGGTAATATTTATGAATTAACATCAGAACATTATGCAAATGAAAATCAATTTGATTTTTCATTGATTAATCAAAAATCTTCTACTTCTACTAATTTATTAGCTTCAAATATTATTTTTAGTATTAACAAAAAGGTGCAATCAATTCTTGTTTTTGAAGATGAAATTCGTGCTGATGCTTATGAAACAATTAAGGTATTGCATGAAAATAATATTGAAACTTATATGATTACTGGTGATAGTTTTAATGTTGCTCAAAAAATCGCAAAGGAATTAGGAATTAAACATTTTTATGCTCAAGTTAAACCAGAAGAAAAAGCTAACATTATTAAAAAAATTCAAAATGATCAAAAAACTGTAATGTATGTTGGCGATGGTATTAATGATTTATTAGCTTTAAAACAAGCTAATGTTTCTATATCAATTGGTGAAACTAATAAAGCAACTAATGCAGTTGCTGATATTAGTTTAATCAAACCAGATATTTTAAATATTTACAAAGTAATTAAACTAACAAAAATTACTAAGATGTTTATTGTAAGTAGTTTACTATGA", "aa": "MAILAFLLQAFVPSIQELPIAFLNLHGSNNDSNLYEKAAYVAVSVLVISCPCAFGIAVPLAVLIGAGHGAKSGITFNNSNIFEKIKKVNAIAFDKTGTLTYGKLQLKQVIGNDQFLDLIYQMELISLHPLAKSFVTYAIINNIVMSTKLIDIKEVAGVGIIAKDVDGNIYELTSEHYANENQFDFSLINQKSSTSTNLLASNIIFSINKKVQSILVFEDEIRADAYETIKVLHENNIETYMITGDSFNVAQKIAKELGIKHFYAQVKPEEKANIIKKIQNDQKTVMYVGDGINDLLALKQANVSISIGETNKATNAVADISLIKPDILNIYKVIKLTKITKMFIVSSLL", "aa_hexdigest": "ed03ea7e6a22225afac2d9b756592d28", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 247464, "stop": 249020, "observations": { "mutation": [ 247740, 248811 ], "stop": [ 247740, 248811 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQU0", "query_cov": 1.0, "subject_cov": 0.4922425952045134, "identity": 0.968, "score": 630.0, "evalue": 8.44e-222, "gene_start": 1, "gene_end": 349, "reference_start": 292, "reference_end": 640, "reference_sequence": "MLKNNLIKKFQLLPINKRRYLISLMKTSIALLISIPLMVFEMLFMFKSNLILGLDGYFIYGWIVFVLSIFIVFGLGFSFFKGAFFEVFKWKKPGMSLLVVISTCVAFIYSTYSLISNTIIYEPKLHGFFETACMIIATMSVGQLVSDRIKLKANQDLQSLNNLQVKKYNSYDLNTKQVNEKVVFQAKINEYALVKKGEIVPLDGVLYSQIAEVDESSLTGEARPILKTINNDIIAGSINVGDNFIFKITKLYNDSTIKKIINGVNQIASSKPKIQVVADKISLWFTPFILLMAILAFLLQAFVPSIQELPIAFLNLHGSNNDSNLYEKAAYVAVSVLVISCPCAFGIAVPLAVLIGAGHGAKSGITFNNSNIFEKIKKVNAIAFDKTGTLTYGKLQLKQVIGNDQFLDLIYQMESISLHPLAKSFVTYAIINNISMSTKLIDIKEVAGVGIIAKDIDGNVYELTSEHYANENQFDFSLINQKSSTSTNLLASNIIFSINKKVQSILVFEDEIRADAYETIKVLHENNIETYMITGDNTKVAQKIANELGIKHFYAQVKPEEKANIIKEIQNQQKTVMYVGDGINDLLALKQANVSISIGETNKATNAVADISLIKPDILNIYKVIKLTKTTKMFIVSSLLWAFGYNLIFIPLALIGIIPPFISVLIMTTSDIAVVLNSLIFRLLKMRLVNRKQAHKLDLKIIDKAMLHK" }, "paralog": false, "identity": 0.9652509652509652, "score": 952.0, "evalue": 0.0, "description": "Internal stop codon at 247740, 248811. Nonsense mutation around 247740, 248811.", "psc": { "uniref90_id": "UniRef90_Q9PQU0", "product": "Copper-transporting P-type ATPase", "uniref50_id": "UniRef50_Q9PQU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQU0", "UniRef:UniRef50_Q9PQU0" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQU0" ], "product": "Copper-transporting P-type ATPase" } }, "id": "GPKLONFDEH_438", "locus": "GCA000006625_00405" }, { "type": "cds", "sequence": "contig_1", "start": 248889, "stop": 249020, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAACTAGTGATATTGCTGTTGTATTAAACTCATTAATTTTTAGATTATTAAAAATGCGTCTAGTTAATAAAAAGCAAATTCATAAACTTAATTTAAAAATTATCAATGAGGCTATGTTACATAAATAA", "aa": "MTTSDIAVVLNSLIFRLLKMRLVNKKQIHKLNLKIINEAMLHK", "aa_hexdigest": "f979baabc08d53c27a34423ea7466ae3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5030.161899999999, "isoelectric_point": 10.65547962188721 }, "id": "GPKLONFDEH_439", "locus": "GCA000006625_00406" }, { "type": "cds", "sequence": "contig_1", "start": 249363, "stop": 249632, "strand": "-", "frame": 2, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_004025791.1", "SO:0001217", "UniParc:UPI00000C1BC9", "UniRef:UniRef100_Q9PQT9", "UniRef:UniRef50_P47663", "UniRef:UniRef90_Q9PQT9" ], "nt": "ATGGCAGTAAGCAAACAACAAAAACATGATTTAACAGTTAAATTTGGTGGTTCTGCATCAAATACAGGAAAAACAGAAGTTCAAGTAGCAATCTTAAGTGCTGAAATTGATTCATTAACAACACATATGATTGAAAATAAAAAAGATAAAGCTTCAAAGCGTGGTTTATACAAAAAAGTAGCTCAACGTAAAAAATTATTATCATACTTACAACGTGTTGATATTGAACGTTATCGTGCTTTAATTAAAGAATTAAACTTAAGAGGATAA", "aa": "MAVSKQQKHDLTVKFGGSASNTGKTEVQVAILSAEIDSLTTHMIENKKDKASKRGLYKKVAQRKKLLSYLQRVDIERYRALIKELNLRG", "aa_hexdigest": "9e9d5afee89d48d12579ec11e0742d42", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BC9", "ncbi_nrp_id": "WP_004025791.1", "uniref100_id": "UniRef100_Q9PQT9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BC9", "RefSeq:WP_004025791.1", "UniRef:UniRef100_Q9PQT9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQT9", "uniref90_id": "UniRef90_Q9PQT9" }, "psc": { "uniref90_id": "UniRef90_Q9PQT9", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P47663", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47663" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "GPKLONFDEH_440", "locus": "GCA000006625_00407" }, { "type": "cds", "sequence": "contig_1", "start": 249851, "stop": 250453, "strand": "-", "frame": 3, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "COG:COG0571", "COG:K", "EC:3.1.26.3", "GO:0003723", "GO:0003725", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0010468", "GO:0019843", "GO:0046872", "SO:0001217", "UniRef:UniRef50_Q9PQT8", "UniRef:UniRef90_Q9PQT8" ], "nt": "ATGGACAATAAAAAATTTTTAGATTTTTTAAAACAAAATAGAATTGAACCAAAAAATTTATCTATTTATCTTGAAGCATTAACACATAAATCTTATGCAAACGAGCATAAACTTACTAAAAACTATCAACGTTTAGAATTTTTAGGTGATGCTTGTGTTGAATGGGTAATTAGCAATTTTATTTTTAACTACAAAATTAAAGATAATGAAAAAATGCGTTCTTTAGATGAAGGTGAAATGACTCGAGCGCGTTCTAATATGGTACGAAGCGAGATTTTATCATATGCTGCTAAAGATTTAGGGTTAACTGATTTTTTAATGATTGGTGTTGGTTTAGAACAAGACCAATCAGCTCGTATGGAAAAAATCTATGAAGATATTTTTGAAGCATTTATAGGTGCTGTAGCTCAAGATCAAGGTATTAAAAAAGTTTCATTAATTCTTGAAAAAACTTTAATTAAATATTTTAGAGAAGGCCAAATTAATTATCAAAAAGATTATAAAACGATTTTTCAAGAACAAGCCCAACGTATTAATAAAAAACCTATTATGTATAAATTAGTACGTAATGAAGGCGATAAAAAAGAAGTCCATTTAGTTTGA", "aa": "MDNKKFLDFLKQNRIEPKNLSIYLEALTHKSYANEHKLTKNYQRLEFLGDACVEWVISNFIFNYKIKDNEKMRSLDEGEMTRARSNMVRSEILSYAAKDLGLTDFLMIGVGLEQDQSARMEKIYEDIFEAFIGAVAQDQGIKKVSLILEKTLIKYFREGQINYQKDYKTIFQEQAQRINKKPIMYKLVRNEGDKKEVHLV", "aa_hexdigest": "5c05027f71ae074cdec618d997aaae1b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQT8", "query_cov": 1.0, "subject_cov": 0.8368200836820083, "identity": 1.0, "score": 392.0, "evalue": 2.26e-137, "valid": true, "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_Q9PQT8", "cog_id": "COG0571", "cog_category": "K", "go_ids": [ "GO:0003723", "GO:0003725", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0010468", "GO:0019843", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQT8" ], "product": "Ribonuclease 3" }, "genes": [ "rnc" ], "id": "GPKLONFDEH_441", "locus": "GCA000006625_00408" }, { "type": "cds", "sequence": "contig_1", "start": 250443, "stop": 251192, "strand": "-", "frame": 2, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811", "KEGG:K03621", "SO:0001217", "UniRef:UniRef50_Q9PQT7", "UniRef:UniRef90_Q9PQT7" ], "nt": "ATGCATTTAGCTTTAAGATATTTAAAAGATAAGCAAGCTGATGGAATGTTAACAGCTGGAAATTCTGCTGTTTTTGTTTATAATGCTTATGCTACAATCGGATTATTAGAACATATTAAGAAACCTGCTTTTATGCCTTTTGTTCCAACAATTGATGGTGGTGTTACAAATTTATTAGATGTGGGTGCATCAATTGATGTTGATGGTACAGATCTATTTAATTTTGCAATCATGGCTAATACAATTGCTAAAATGCGTACTCCAAATCCTCGTGTTGGCGTTTTAAATATTGGTACTGAAGATCATAAAGGTTTACCATATCATCAAGAAGCTAATGAATTATTAAAAACATCAAACTTAAATTATGTGGGTTTTGTAGAACCAAAAACAATTTTAGAACGAGAAGTTGATGTTTTAGTTGCTGATGGTTTTAGTGGAAACATTGCTTTAAAAACAATGGAAGGTGTTGGTAAAACAATTTCTAATTTTTTAAAAAATGAATATAAAAAACCAAAAAATCTTTTTGCTGCTTTATTGTCAAAACCAATTTTTAAAAAAATAAAAAAAGCATTTGATTATAAAGAACATGCTGGAGCTTTTGTTTTAGGATTAGATGGCATTTTAGTTAAAACCCATGGTAGTGCTGATTACCAACAATTTATGAGTGCTTTAAAAATTTTGTATGAAACAATTAAAGCAGATGTTTTAAATGAAATTAAAAAAGATTTAAATAATTATTATGGACAATAA", "aa": "MHLALRYLKDKQADGMLTAGNSAVFVYNAYATIGLLEHIKKPAFMPFVPTIDGGVTNLLDVGASIDVDGTDLFNFAIMANTIAKMRTPNPRVGVLNIGTEDHKGLPYHQEANELLKTSNLNYVGFVEPKTILEREVDVLVADGFSGNIALKTMEGVGKTISNFLKNEYKKPKNLFAALLSKPIFKKIKKAFDYKEHAGAFVLGLDGILVKTHGSADYQQFMSALKILYETIKADVLNEIKKDLNNYYGQ", "aa_hexdigest": "2dff98ced4be68873ec47d6f94cc9f1d", "start_type": "ATG", "rbs_motif": null, "genes": [ "plsX" ], "truncated": "3-prime", "pseudogene": { "start": 250443, "stop": 251438, "observations": { "mutation": [ 251279 ], "stop": [ 251279 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQT7", "query_cov": 1.0, "subject_cov": 0.7522658610271903, "identity": 1.0, "score": 495.0, "evalue": 9.36e-176, "gene_start": 1, "gene_end": 249, "reference_start": 83, "reference_end": 331, "reference_sequence": "MEKIKILLDTMGYENDLEHVIKAAKDFYYQHEDDLEIILVGNEQLIKPLLDNDWRLFPIVHTEVSIEQNDTILSARKKQNSSMHLALRYLKDKQADGMLTAGNSAVFVYNAYATIGLLEHIKKPAFMPFVPTIDGGVTNLLDVGASIDVDGTDLFNFAIMANTIAKMRTPNPRVGVLNIGTEDHKGLPYHQEANELLKTSNLNYVGFVEPKTILEREVDVLVADGFSGNIALKTMEGVGKTISNFLKNEYKKPKNLFAALLSKPIFKKIKKAFDYKEHAGAFVLGLDGILVKTHGSADYQQFMSALKILYETIKADVLNEIKKDLNNYYGQ" }, "paralog": false, "identity": 0.9969788519637462, "score": 653.0, "evalue": 1.66e-240, "description": "Internal stop codon at 251279. Nonsense mutation around 251279.", "psc": { "uniref90_id": "UniRef90_Q9PQT7", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q9PQT7", "cog_id": "COG0416", "cog_category": "I", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQT7", "EC:2.3.1.274", "UniRef:UniRef50_Q9PQT7", "COG:COG0416", "COG:I", "KEGG:K03621", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQT7" ], "product": "Phosphate acyltransferase" } }, "id": "GPKLONFDEH_442", "locus": "GCA000006625_00409" }, { "type": "cds", "sequence": "contig_1", "start": 251277, "stop": 251438, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAAATTAAAATTTTATTAGATACAATGGGTTATGAAAATGATTTAGAACATGTAATTAAAGCAGCTAAAGATTTTTATTATCAACATGAAGATGATTTAGAAATTATCTTAGTAGGAAATGAACAATTAATTAAGCCATTATTAGATAATGATTGA", "aa": "MEKIKILLDTMGYENDLEHVIKAAKDFYYQHEDDLEIILVGNEQLIKPLLDND", "aa_hexdigest": "b24a1f5275bc1358d222e3ee1f02f4ec", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6248.0486999999985, "isoelectric_point": 4.382252311706543 }, "id": "GPKLONFDEH_443", "locus": "GCA000006625_00410" }, { "type": "cds", "sequence": "contig_1", "start": 251443, "stop": 253137, "strand": "-", "frame": 1, "gene": "fakA", "product": "DhaL domain-containing protein", "db_xrefs": [ "COG:COG1461", "COG:I", "RefSeq:WP_006688935.1", "SO:0001217", "UniParc:UPI00000C1BCA", "UniRef:UniRef100_A0A2C9DYP5", "UniRef:UniRef50_A0AAC9T074", "UniRef:UniRef90_A0AAC9T074" ], "nt": "ATGAAAACAATTAAGTTAGATCTTTTTCAAAAAATGTTATATGCAGGTGCAAAAATATTAGAAATTGAATGTGAATATATTAACGATTTAAATGTTTTTCCTGTTCCAGATGGTGATACTGGTTCAAACATGAAAACCACAACAGCTGGTGCAATTATTGCTATTAAAGAGATAGAAATATCCAATTTTTCCCAACTTGCTAAAATTTTTAGTCGTGGTCTATTAATGAACGCCAGAGGTAACTCTGGTGTTATTTTAAGTCAAATATTTCGTGGATTTATGGATGCTTTAAATAACGATGCTTATGAAGTTAGTATTGAACAATTAAAACAAGCTTTTAATGGTGCAAAAGAAAAAGCTTATGCAAGCGTCTCTACACCTATTGAAGGAACAATTTTAACAGTCATTCGCGTTATTAATGAGCGAATAAGTGAACGTCAAAATTTTGATAGTGTCGAAGATTTATTTGCATTTGTTACAAAAGAAGCGCAAATCATTCTAGAAAGAACACCAGAAATGTTACCTTCGCTAAAAGAAGCTAATGTTGTTGATTCTGGTGGATATGGTTTAGTAAGTTTTCTAAAAGGAATGTATCAAAGCTTAACAAATACATTTGATGAAAAATTAATTATTAAAAACGAAGAAGAAAATATTAAATCAAAGACTAAACCGCTTGATCTTGAAATTATACATAAAGAAAAAGAAGAAGGTTTTGGTTATTGTAGTGAAATTATTTTATCTTTACGAAAAAAAATTATCCCTAATTCACCAAGTAAAAGTTTTTTTGAATTTGAAAAATATAAAAGTGAATTAGCAAAAATCGGTGATAGTATTGTAGCAATCCAAGATGAAGATCTAGTTAAAGTTCATTTGCATACCCTAACTCCTTATAAATTCTTACAATTATCACAAAAATATGGTGAATTTGATAAGATTAAAATTGAAAACATGACAAACCAATTTTATGAAAGTTTAGAACGAAAAGGGTTGACTACAAAAAAAGAAAATAAATTGACTTTAAGTATGGAGCAAAAAATTATTGCAACAGTTCCATCTGAAAAGTTTAAAAAAATGTTTATTGAAGAACATGGTATTGATAATGTTATTGTTACTGAAACAGAAGGAGCTCCATCAATTCAACGTTTTGTCGATGAAATTAACTCAACAAACTCTTCACAAATTTTTATTGTCACTGATGATTCAAATATTGTATTAGCTGCTGAACAAGCTTCAAAAATCGTTGCTGAAAACAACATTTTGGTTTCTGTAATTGGAGCTTATAATGTTTTTGAAACGCTTTTAGCGATTTCTCATTTTGAACAGCAAAATGATTATCGCACAAATTTTAAAACAATGAATAAAATAGTTAAAAAAGCACGTAGTGGGATGATTACTACTTCAATTAAAAATGTTAAATATCCACATATTACTGTTAATAAAGATGATTATATAGGCATTATTAATAAAAAAATTATCGCTTCTGAAAAAAATGCTTATGATGTTTTATTAAAAACAATTGAAATGTTAATTGAAAGTTTAAAAAAACCAGAAATTTGTTATATATATTATGGCATTAATGCTTCACAAACAGATGTACATCAAATTGAAAAATACATTTCAGAAAAATATGGAATTATTTGTGATTTAAGATATACTGGTCAAAGTTTATATCATTACTACATAGGAATTCAATAA", "aa": "MKTIKLDLFQKMLYAGAKILEIECEYINDLNVFPVPDGDTGSNMKTTTAGAIIAIKEIEISNFSQLAKIFSRGLLMNARGNSGVILSQIFRGFMDALNNDAYEVSIEQLKQAFNGAKEKAYASVSTPIEGTILTVIRVINERISERQNFDSVEDLFAFVTKEAQIILERTPEMLPSLKEANVVDSGGYGLVSFLKGMYQSLTNTFDEKLIIKNEEENIKSKTKPLDLEIIHKEKEEGFGYCSEIILSLRKKIIPNSPSKSFFEFEKYKSELAKIGDSIVAIQDEDLVKVHLHTLTPYKFLQLSQKYGEFDKIKIENMTNQFYESLERKGLTTKKENKLTLSMEQKIIATVPSEKFKKMFIEEHGIDNVIVTETEGAPSIQRFVDEINSTNSSQIFIVTDDSNIVLAAEQASKIVAENNILVSVIGAYNVFETLLAISHFEQQNDYRTNFKTMNKIVKKARSGMITTSIKNVKYPHITVNKDDYIGIINKKIIASEKNAYDVLLKTIEMLIESLKKPEICYIYYGINASQTDVHQIEKYISEKYGIICDLRYTGQSLYHYYIGIQ", "aa_hexdigest": "48d66fed08fcbcf996ad8d4d7146cd31", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1BCA", "ncbi_nrp_id": "WP_006688935.1", "uniref100_id": "UniRef100_A0A2C9DYP5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BCA", "RefSeq:WP_006688935.1", "UniRef:UniRef100_A0A2C9DYP5" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYP5", "uniref90_id": "UniRef90_A0AAC9T074" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T074", "gene": "FakA", "product": "DhaL domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T074", "cog_id": "COG1461", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T074" ], "product": "DhaL domain-containing protein" }, "genes": [ "dhaL", "fakA" ], "id": "GPKLONFDEH_444", "locus": "GCA000006625_00411" }, { "type": "cds", "sequence": "contig_1", "start": 253152, "stop": 253418, "strand": "-", "frame": 2, "gene": null, "product": "Asp23/Gls24 family envelope stress response protein", "db_xrefs": [ "RefSeq:WP_006688901.1", "SO:0001217", "UniParc:UPI00000C1BCB", "UniRef:UniRef100_A0AAC9T359", "UniRef:UniRef50_A0A084EXH6", "UniRef:UniRef90_A0AAC9T359" ], "nt": "ATGTTAAAAAAAACTAAATTTGATACTCAACTTGTTAAAAGTTTAATTACATCCTCTATTTTAAAAGTTCCTGGGATTTTTAGTGTTGACATAAATGACAAATTATCAGATTTTAATCGTAATTATTTTGTAATTAAAATTGATTTACATGAAGATGTTGTTAATGTTTTAAGTGTAGCAAATGAAGCGCGTAATTTAGTATATTATGAATTGTCAAAACAATTAAATGATGATAATGTGGTTATTAACATCATTATAAACTGTTAA", "aa": "MLKKTKFDTQLVKSLITSSILKVPGIFSVDINDKLSDFNRNYFVIKIDLHEDVVNVLSVANEARNLVYYELSKQLNDDNVVINIIINC", "aa_hexdigest": "418a95796f58a071b86e6bedc031709a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BCB", "ncbi_nrp_id": "WP_006688901.1", "uniref100_id": "UniRef100_A0AAC9T359", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BCB", "RefSeq:WP_006688901.1", "UniRef:UniRef100_A0AAC9T359" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T359", "uniref90_id": "UniRef90_A0AAC9T359" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T359", "product": "Asp23/Gls24 family envelope stress response protein", "uniref50_id": "UniRef50_A0A084EXH6" }, "pscc": { "uniref50_id": "UniRef50_A0A084EXH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EXH6" ], "product": "Asp23/Gls24 family envelope stress response protein" }, "genes": [], "id": "GPKLONFDEH_445", "locus": "GCA000006625_00412" }, { "type": "cds", "sequence": "contig_1", "start": 253523, "stop": 253765, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307DFEB7", "UniRef:UniRef90_UPI00307DFEB7" ], "nt": "TTGATTAATAATTCCAATCCACAAATTTTACTATTTGAAATTTCAAGAAATAAAAAGCAAAAATATCATAGTTTATTACTTTTTGCTGATAAAAATGTATTGAAAAAATTTTATTTAAATTCTGCAACAAAAGATAATTATGAAGATATTATTGATTTGGCCTTTAATAAATTTGAAAATTTACAAAATTTATTAAAAACAACAAAAGTTAAAAACCATTTAAAAAATTTGAAAAAGTATTAA", "aa": "MINNSNPQILLFEISRNKKQKYHSLLLFADKNVLKKFYLNSATKDNYEDIIDLAFNKFENLQNLLKTTKVKNHLKNLKKY", "aa_hexdigest": "206e2e1bd36b2a3628e12227469b9eea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 253523, "stop": 253957, "observations": { "mutation": [ 253798 ], "stop": [ 253798 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00307DFEB7", "query_cov": 1.0, "subject_cov": 0.5555555555555556, "identity": 0.988, "score": 155.0, "evalue": 3.32e-47, "gene_start": 1, "gene_end": 80, "reference_start": 65, "reference_end": 144, "reference_sequence": "MINKNDLLKENFIYKDIYINLFQIYVTKKNNLSRYLSNQFNFTLTKNDFKDELWNKLIYNTIKKLINNSNPQILLFEISRNKKQKYHSLLLFADKNVLKKFYLNSATKDNYEDIIDLAFNKFENLQNLLKTTKVKNHLKNLKKY" }, "paralog": false, "identity": 0.9861111111111112, "score": 285.0, "evalue": 7.06e-101, "description": "Internal stop codon at 253798. Nonsense mutation around 253798.", "psc": { "uniref90_id": "UniRef90_UPI00307DFEB7", "uniref50_id": "UniRef50_UPI00307DFEB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00307DFEB7", "UniRef:UniRef50_UPI00307DFEB7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00307DFEB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307DFEB7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9561.028599999992, "isoelectric_point": 9.80327205657959 }, "id": "GPKLONFDEH_446", "locus": "GCA000006625_00413" }, { "type": "cds", "sequence": "contig_1", "start": 253796, "stop": 253957, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCAATAAAAATGACTTATTAAAAGAAAATTTTATTTATAAGGATATTTATATTAATTTATTTCAAATCTATGTTACTAAAAAAAATAATTTAAGCAGATATTTAAGTAACCAGTTTAATTTTACGTTAACAAAAAACGATTTTAAAGACGAATTGTGA", "aa": "MINKNDLLKENFIYKDIYINLFQIYVTKKNNLSRYLSNQFNFTLTKNDFKDEL", "aa_hexdigest": "d206e61713d938c70f3b1a7b8bdecb37", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6527.4128, "isoelectric_point": 9.004250907897951 }, "id": "GPKLONFDEH_447", "locus": "GCA000006625_00414" }, { "type": "cds", "sequence": "contig_1", "start": 253976, "stop": 254140, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAAGAGATTTTAATAAGAAATAGTGATTTAAATTATATTGTTAGTGATCCTTTTGAAAACATGATTGGAGGTTTTCCTATTATTTTATTTGAAAATGATAATTATATATATTATTTATCTGGTCAAAATGCTTATTTACCTTCAAAAATGCGTCGTTGA", "aa": "MYKEILIRNSDLNYIVSDPFENMIGGFPIILFENDNYIYYLSGQNAYLPSKMRR", "aa_hexdigest": "1e992452277769364260b2000be80394", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6441.323400000001, "isoelectric_point": 4.992987251281736 }, "id": "GPKLONFDEH_448", "locus": "GCA000006625_00415" }, { "type": "cds", "sequence": "contig_1", "start": 255370, "stop": 255492, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAATAAATCATTATCCAAACGCTTTATTAGTAAAAGGTCATGCAAATTTTGATGAGCATGGCAAAGATATAGTATGCGCTGGAGTGAGTGCTATTATTATGGGTGCACTTAATTGA", "aa": "MIKINHYPNALLVKGHANFDEHGKDIVCAGVSAIIMGALN", "aa_hexdigest": "b917f162790c6e7ae7c6b2c53a7b3184", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4275.972199999998, "isoelectric_point": 6.80529842376709 }, "id": "GPKLONFDEH_449", "locus": "GCA000006625_00416" }, { "type": "cds", "sequence": "contig_1", "start": 255498, "stop": 255800, "strand": "-", "frame": 2, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02888", "RefSeq:WP_006688851.1", "SO:0001217", "UniParc:UPI00000C1BCE", "UniRef:UniRef100_Q9PQT0", "UniRef:UniRef50_Q9PQT0", "UniRef:UniRef90_Q9PQT0" ], "nt": "ATGTTTGCTATTTTTCAAACAGGCGGAAAACAATACAAAGTTCAACAAGGCGAAAAAATCTATGTTGAAAAACTAGATTTAGAAGTTGGTAGTAAAATTTCATTTGATCAAGTTATTATGGTAGAAGGTAGTGTTGGTACTCCTTTTGTAAAAAATGCTGTAGTTAATGCTACTGTTATAAAACAAGGAAAACAAAAGAAGATCAACATTATTAAATTTAAATCTAAAAAACACCATTTAAAACGTCAAGGTCATCGACAACCATATACACAATTAGTTATTGATTCAATTAGTGTTAAGTAG", "aa": "MFAIFQTGGKQYKVQQGEKIYVEKLDLEVGSKISFDQVIMVEGSVGTPFVKNAVVNATVIKQGKQKKINIIKFKSKKHHLKRQGHRQPYTQLVIDSISVK", "aa_hexdigest": "41b89912f33dbdcdcb2aad6b7e65a98c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BCE", "ncbi_nrp_id": "WP_006688851.1", "uniref100_id": "UniRef100_Q9PQT0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BCE", "RefSeq:WP_006688851.1", "UniRef:UniRef100_Q9PQT0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQT0", "uniref90_id": "UniRef90_Q9PQT0" }, "psc": { "uniref90_id": "UniRef90_Q9PQT0", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_Q9PQT0", "cog_id": "COG0261", "cog_category": "J", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQT0" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "GPKLONFDEH_450", "locus": "GCA000006625_00417" }, { "type": "ncRNA-region", "class": [ "attenuator", "SO:0000140" ], "sequence": "contig_1", "start": 255828, "stop": 255903, "strand": "-", "label": "L21_leader", "product": "Ribosomal protein L21 leader", "score": 60.1, "evalue": 1.8e-07, "db_xrefs": [ "RFAM:RF00559", "SO:0000140" ], "nt": "ATGCAGCTTCAACCACTCAAAAAAGGTTTAAAAATAAATGTTAATTTATACCTTATTGGTGAGTCTAAGTTGATAA", "id": "GPKLONFDEH_41" }, { "type": "cds", "sequence": "contig_1", "start": 256118, "stop": 256336, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00625.27" ], "nt": "ATGAAACGCGGAAAATTAATTGTTTTTAGTGGACCTAGTGGTGTAGGAAAACATACAATTCTATCTAAAATCATAAATCGTAAAGATTTAAATTTAGCATATAGTATTTCAATGACAACCCGAAAAAAACGTGAAGGTGAAGTTAATGGTGTTGATTATTATTTTGTTAATGATGAAGAATTTAAAAAGGCCATACTAAATAATGAATTAATTGAGTGA", "aa": "MKRGKLIVFSGPSGVGKHTILSKIINRKDLNLAYSISMTTRKKREGEVNGVDYYFVNDEEFKKAILNNELIE", "aa_hexdigest": "6e9b31d227d796d53c80bf9e2c1c25e4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00625.27", "name": "Guanylate_kin", "length": 69, "aa_cov": 0.9583333333333334, "hmm_cov": 1.0, "evalue": 5.0820022611864367e-23, "score": 77.28531646728516, "start": 4, "stop": 72 } ], "seq_stats": { "molecular_weight": 8218.4237, "isoelectric_point": 9.520965003967284 }, "id": "GPKLONFDEH_451", "locus": "GCA000006625_00418" }, { "type": "cds", "sequence": "contig_1", "start": 256674, "stop": 257243, "strand": "+", "frame": 3, "gene": "rlmN", "product": "Dual-specificity RNA methyltransferase RlmN N-terminal domain-containing protein", "db_xrefs": [ "COG:COG0820", "COG:J", "EC:2.1.1.192", "KEGG:K06941", "SO:0001217", "UniRef:UniRef50_A0AAC9X6G4", "UniRef:UniRef90_A0AAC9X6G4" ], "nt": "ATGAAATTAAACAATCATAATCTAAAAGAAATTTATTCATTATCATTAAATGAACTTAAAGAAGAATTATTTAAATTAGGCTTAAAATCTTTTATTAGCAAACAAATTTATTCTTGGATTTATCAGAAACGAATTTTTAATTTTGATAAATTTTCTAATATTGCTAAAAATAATGTAATAATTCTAAAAGAAAATTTTAGTAATAATCTATTAAAAATTAACAGTTATCAATCAAATTCTGATGGTTCAATTAAGTTTGAATTTTTAACAGATAAAAATCTGATTATTAATTGCATGATTATTAAATTTAAAAACGGATTTTTAATAAAAATAAATCCGTTTGGAATTAATTTTAAAAAAGAAATTATTAATTTATCAACTAATGAATTAGTATTACAAATTTTATTAATACAACAATTTCTAGATCAACGTAAATTAGGCAACATTACTAATGTTATTATAAAAGGATTACAGGATAGCTTATTAAATATGGACGTTGTATCAAACTTCATTAATATTATTAATAATGAAAATGGTTTAAATATTGGAAAAAGGAAAATTGCAGTTTGA", "aa": "MKLNNHNLKEIYSLSLNELKEELFKLGLKSFISKQIYSWIYQKRIFNFDKFSNIAKNNVIILKENFSNNLLKINSYQSNSDGSIKFEFLTDKNLIINCMIIKFKNGFLIKINPFGINFKKEIINLSTNELVLQILLIQQFLDQRKLGNITNVIIKGLQDSLLNMDVVSNFINIINNENGLNIGKRKIAV", "aa_hexdigest": "f54a92f590ae3445dc5b2fb812cdb437", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rlmN" ], "truncated": "3-prime", "pseudogene": { "start": 256674, "stop": 257546, "observations": { "mutation": [ 257241, 257274, 257373 ], "stop": [ 257241, 257274, 257373 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6G4", "query_cov": 1.0, "subject_cov": 0.5625, "identity": 1.0, "score": 358.0, "evalue": 7.5e-123, "gene_start": 1, "gene_end": 189, "reference_start": 1, "reference_end": 189, "reference_sequence": "MKLNNHNLKEIYSLSLNELKEELFKLGLKSFISKQIYSWIYQKRIFNFDKFSNIAKNNVIILKENFSNNLLKINSYQSNSDGSIKFEFLTDKNLIINCMIIKFKNGFLIKINPFGINFKKEIINLSTNELVLQILLIQQFLDQRKLGNITNVIIKGLQDSLLNMDVVSNFINIINNENGLNIGKRKIAVWTSGINVDLIKWGQLQNQIELIISMNASNSLIYQKIMLKKLNQNWSFLKLIEQIKAYTKITNNRVVLEYLLIDKINDDLNYANELVELLKNILCYVLLIPYNLNNYRTSANLDDFFNILSVNKIRISKRIRKSNDLDISFKQLKIKE" }, "paralog": false, "identity": 0.9896551724137931, "score": 551.0, "evalue": 4.34e-201, "description": "Internal stop codon at 257241, 257274, 257373. Nonsense mutation around 257241, 257274, 257373.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6G4", "gene": "RlmN", "product": "Dual-specificity RNA methyltransferase RlmN N-terminal domain-containing protein", "ec_ids": [ "2.1.1.192" ], "uniref50_id": "UniRef50_A0AAC9X6G4", "cog_id": "COG0820", "cog_category": "J", "kegg_orthology_id": "K06941", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6G4", "EC:2.1.1.192", "UniRef:UniRef50_A0AAC9X6G4", "COG:COG0820", "COG:J", "KEGG:K06941" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6G4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6G4" ], "product": "Dual-specificity RNA methyltransferase RlmN N-terminal domain-containing protein" } }, "id": "GPKLONFDEH_452", "locus": "GCA000006625_00419" }, { "type": "cds", "sequence": "contig_1", "start": 257526, "stop": 257684, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTTAATTCCTTATAATTTAAATAATTATCGAACTAGTGCTAATTTAGATGATTTTTTTAATATTTTATCTGTTAATAAAATACGAATTAGTAAGCGTATACGAAAAAGTAACGATTTAGATATTAGTTTTAAACAATTGAAAATTAAGGAGTAA", "aa": "MLLIPYNLNNYRTSANLDDFFNILSVNKIRISKRIRKSNDLDISFKQLKIKE", "aa_hexdigest": "af46c081bbcbd00ae9b6fac296164635", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6215.187599999999, "isoelectric_point": 9.991777610778808 }, "id": "GPKLONFDEH_453", "locus": "GCA000006625_00420" }, { "type": "cds", "sequence": "contig_1", "start": 257688, "stop": 258431, "strand": "+", "frame": 3, "gene": "pTC1", "product": "Protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "KEGG:K20074", "RefSeq:WP_006688864.1", "SO:0001217", "UniParc:UPI00000C1BD0", "UniRef:UniRef100_Q9PQS7", "UniRef:UniRef50_Q9PQS7", "UniRef:UniRef90_Q9PQS7" ], "nt": "ATGAATTTTGGGTTTATTAGTGATATTGGCTCACAACGCAAACATAATGATGATTGTGCATTGGTAATTCAAAATCAATATCAACAAACTTTATTAATTGTTTGTGATGGATTAGGCGGGTATAAAGGTGGTGCTGCGGCTAGCCACATTACATTAGAAACAATTAAAGATAATTTTTTAACTACAGATTTTAGTGAGTATGATAAACAACAGATTCAAAAATGGTATATTAAAGTTATTAAACTAGCACAGGTTGAAATTGATCGCACTGTTTTACTAGATAAAGACGTTTATAATATGGGAACTACAGTTGTTGCTTCTATTGTTGTTAATGATTTTTCATACACTCTAAATATTGGTGATTCGCGTGCTTATTTATTATCCAATGAACAATTCTTACAAATAAGTCGCGATCATAATTTATTACAAGTTTTACAAGAACGTAAAGCCAACCCTGAGGTTTATAAAAAACATGAGAAGAATTTATTTTCATTAACACAATTTGTTGGTCGCACAAGTAATATTTTTTTGAGTTATGACTTATTCGTAAATAAATTAAACCCAAATGAAATTATTGTTTTAACATCAGATGGATTCCATAACTATTTTGAATTAAATACATTATATGAAAAATTAATAAAAAGCACCCAAGAGACAAAAAATGAACTTTTACAACAATTAATAAGACAAGCTATTATCAACGGTAGCAATGATAATTTATCATTAGCTTTCTTAATTTTTTAA", "aa": "MNFGFISDIGSQRKHNDDCALVIQNQYQQTLLIVCDGLGGYKGGAAASHITLETIKDNFLTTDFSEYDKQQIQKWYIKVIKLAQVEIDRTVLLDKDVYNMGTTVVASIVVNDFSYTLNIGDSRAYLLSNEQFLQISRDHNLLQVLQERKANPEVYKKHEKNLFSLTQFVGRTSNIFLSYDLFVNKLNPNEIIVLTSDGFHNYFELNTLYEKLIKSTQETKNELLQQLIRQAIINGSNDNLSLAFLIF", "aa_hexdigest": "20c79ef7bfabe59bf79301183229558b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BD0", "ncbi_nrp_id": "WP_006688864.1", "uniref100_id": "UniRef100_Q9PQS7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BD0", "RefSeq:WP_006688864.1", "UniRef:UniRef100_Q9PQS7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQS7", "uniref90_id": "UniRef90_Q9PQS7" }, "psc": { "uniref90_id": "UniRef90_Q9PQS7", "gene": "PTC1", "product": "Protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_Q9PQS7", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074" }, "pscc": { "uniref50_id": "UniRef50_Q9PQS7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQS7" ], "product": "Protein phosphatase" }, "genes": [ "pTC1" ], "id": "GPKLONFDEH_454", "locus": "GCA000006625_00421" }, { "type": "cds", "sequence": "contig_1", "start": 258437, "stop": 259228, "strand": "+", "frame": 2, "gene": "sPS1", "product": "Serine/threonine kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "SO:0001217", "UniRef:UniRef50_Q9PQS6", "UniRef:UniRef90_Q9PQS6" ], "nt": "ATGCGTGAAGAAATAAAAACAAACTCAATTCTTAATTATAAATATAAAGTTGTTAAACACCTAGCAGATGGTGGTTTTAGTAAAGTTTATTTATGTTGTTTTTTATCAGATGAAACTAAGTTTATTGTTATTAAAGTTTTAGATATTAATGATGAGAAACAACAAATGGTTGTTTATGATGAATTAAGAATTTCAAATTTAATTAAAAATTCTGATTCTGATAAAAAAAACTATATTATGGAATATTATGAATATTTTGAAACCGGTTCGTTAGAAAATAATGATAAAAGGATTTATATTGTTTTTGAATACATTGAAGGTTTGACATTGCGTGAATATCTTGATGAATTTAAAACAATTACTTATGTTAAGGCTGTTGAAATTATTCGTCAGGTTGCCTTAGGTGTAAGTTTTTTTCATAGTTGTAATCCACAAATTATTCATCGTGATTTAAAGCCAGAAAATTGCATGATTAATAAAACCTTAACAAAAATTAAAATTATTGATTATGGTGCAGCATCGGTTTTTTACAATCGTGAAGATTTAACAAAAGATCAAGAAATTAAATGTACAATTATTTACGCTTCACCAAAATTATTAAGTCTTGGGCAAAAAGTTAAAGAACAAGCTAATAAGGGTTTAAATAAAAGAGCTTTAAGTTTAATTAATGACGCATTAGGTGTTAGTTATGATATTCATTCTTTAGGAGTTATGTTATATGAATTAATTACTGGAACTAACCCTTTTAGTGAACATACAATTACAGATGATCGTGATTATTTAGAAAAATGA", "aa": "MREEIKTNSILNYKYKVVKHLADGGFSKVYLCCFLSDETKFIVIKVLDINDEKQQMVVYDELRISNLIKNSDSDKKNYIMEYYEYFETGSLENNDKRIYIVFEYIEGLTLREYLDEFKTITYVKAVEIIRQVALGVSFFHSCNPQIIHRDLKPENCMINKTLTKIKIIDYGAASVFYNREDLTKDQEIKCTIIYASPKLLSLGQKVKEQANKGLNKRALSLINDALGVSYDIHSLGVMLYELITGTNPFSEHTITDDRDYLEK", "aa_hexdigest": "bbdca21c34a0a0871aa52ff314662f16", "start_type": "ATG", "rbs_motif": null, "genes": [ "sPS1" ], "truncated": "3-prime", "pseudogene": { "start": 258437, "stop": 259528, "observations": { "mutation": [ 259226, 259313, 259493, 259508 ], "stop": [ 259226, 259313, 259493, 259508 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQS6", "query_cov": 1.0, "subject_cov": 0.6813471502590673, "identity": 0.932, "score": 494.0, "evalue": 1.68e-174, "gene_start": 1, "gene_end": 263, "reference_start": 1, "reference_end": 263, "reference_sequence": "MREQIQTNSILNHKYKVVKHLADGGFSKVYLCCFLSDETKFIVVKVLDIGDEKQQMVVYDELRISNLIKNSNSDKRSYIMEYYEYFESGSLETDDKRIYIVFEYIDGLTLREYLDEFKTVTYVKAVEIIRQVALGVSFFHSCNPQIIHRDLKPENCMINKTLSKIKIIDYGAASVFYNREDLTKDQEIKCTIIYASPKLLSLGQKVKEQASKGLNKNALSLINDALGVNYDIHSLGVMLYELITGTNPFSEHTIKDDRDYLEKWTTYDVEPLSSINKTIPKGIDNILIRCFAWKKEDNKLLYKDIYTLIDDLNNVMDPESSLNQDYIKPLNKLRIYRKNVAGLYEIKGKDRKWYLQKWFIILVGCLSIILIILLIIILSFKKSGAI" }, "paralog": false, "identity": 0.9146005509641874, "score": 667.0, "evalue": 7.36e-245, "description": "Internal stop codon at 259226, 259313, 259493, 259508. Nonsense mutation around 259226, 259313, 259493, 259508.", "psc": { "uniref90_id": "UniRef90_Q9PQS6", "gene": "SPS1", "product": "Serine/threonine kinase", "uniref50_id": "UniRef50_Q9PQS6", "cog_id": "COG0515", "cog_category": "T", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQS6", "UniRef:UniRef50_Q9PQS6", "COG:COG0515", "COG:T" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQS6" ], "product": "Serine/threonine kinase" } }, "id": "GPKLONFDEH_455", "locus": "GCA000006625_00422" }, { "type": "cds", "sequence": "contig_1", "start": 259334, "stop": 259495, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATACAAAGATATCTACACTTTAATTGATGATTTAAGTAATGTTATGGATCCAGAGTCAAATCTTAATCAAGAATACATTAAACCATTAAATAAGTTACGAATTTATCGTAAAAATCTAGCAGGCTTATATGAAATTAAAGAAAAAGATCGTAAATGA", "aa": "MLYKDIYTLIDDLSNVMDPESNLNQEYIKPLNKLRIYRKNLAGLYEIKEKDRK", "aa_hexdigest": "9b1e9486c2d1323a2d05da69479def14", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6420.3696, "isoelectric_point": 7.942392158508301 }, "id": "GPKLONFDEH_456", "locus": "GCA000006625_00423" }, { "type": "cds", "sequence": "contig_1", "start": 259600, "stop": 260421, "strand": "+", "frame": 1, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872", "SO:0001217", "UniRef:UniRef50_Q9PQS5", "UniRef:UniRef90_Q9PQS5" ], "nt": "GTGCGGGCAAAGATTATTTCGGTTGTTGCTAATAATTTTTATGTACATCTTTATGATTTAAAAATTCAGATAAAAGCAATTCCCAAAGGGATTTTTAAACATAACAGTCATGAATTAAAACCAATGGTTGGGGATGATGTTGAAGTTGAATTAATTGGGAATACTTATTTAATCACAAAAATTTATGAACGTTATAATCAATTAATTCGTCCCAAAGTTGCAAATATTGATATTGTTTTAATCGTAGTCTCTATTGTACAACCAGATTTAAATACATTGAGTTTAAACAAATATTTAGCTTTTTATGAAGCTCGCAATATTAAAAATGTTTTAATAGGTTTGAGTAAATATGATTTAGCCAATAATCATTTAAAACAAAAAATTAATAAGTTAATTTTAGATTATAAAAAAAATAACTATGCGGTTTTTATATTAACAAAAGAAAATGATATTTTATTATTAAAAGAAAAGATTAAAAGCAAGACACTTTGTTTGGCAGGAAATTCAGGCGTAGGTAAATCAACATTAATTAATAAATTAGATCCAAGCATTCAACAACGTACACAGGAAATATCACATTTTCTGAATCGAGGAAAACACACAACAACAAGTACCAAACTAATTAGTTTTGCTAATGGCTTTTTAGTTGATACACCGGGTTTTGGAAATTTAGAGGTTAATTTAACCAAGAATGAAATGGCTAATGCCTTTAATGATTTTGCAAATTATGCACGTTTTTGTAAATTTTCTAATTGTTTACATATTGAAGAACCAGAATGTGCTATTAAAAAAGCTGTTGATGAAAAATTAATTGTTAATTGA", "aa": "MRAKIISVVANNFYVHLYDLKIQIKAIPKGIFKHNSHELKPMVGDDVEVELIGNTYLITKIYERYNQLIRPKVANIDIVLIVVSIVQPDLNTLSLNKYLAFYEARNIKNVLIGLSKYDLANNHLKQKINKLILDYKKNNYAVFILTKENDILLLKEKIKSKTLCLAGNSGVGKSTLINKLDPSIQQRTQEISHFLNRGKHTTTSTKLISFANGFLVDTPGFGNLEVNLTKNEMANAFNDFANYARFCKFSNCLHIEEPECAIKKAVDEKLIVN", "aa_hexdigest": "3381ab71c5070bfe1183c1c96eb6fd3d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQS5", "query_cov": 1.0, "subject_cov": 0.900990099009901, "identity": 1.0, "score": 530.0, "evalue": 1.73e-189, "valid": true, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.6.1.-" ], "uniref50_id": "UniRef50_Q9PQS5", "cog_id": "COG1162", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQS5" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" }, "genes": [ "rsgA" ], "id": "GPKLONFDEH_457", "locus": "GCA000006625_00424" }, { "type": "cds", "sequence": "contig_1", "start": 260729, "stop": 260932, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAGCAACAGCTGATGCTAATACATTAGCATTAACTAATTTTAATCCGACTAGCACTTTATTAGTTGCATTAGCAATTGCCTTTGTCTTATTGATGACTCCAGGTTTAGCATTATTTTATGGTGGGCTAACACGTCGAAAATCTACATTAACGATTATTAATCAATGTGTAGCTTCACTTGGAATCACAACACTGATTTGA", "aa": "MPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLALFYGGLTRRKSTLTIINQCVASLGITTLI", "aa_hexdigest": "e2a728ad2b50d61580bd48a565ecd5fe", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6995.271999999998, "isoelectric_point": 9.301901435852049 }, "id": "GPKLONFDEH_458", "locus": "GCA000006625_00425" }, { "type": "cds", "sequence": "contig_1", "start": 261785, "stop": 262102, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGTGTTACTGGTTCATTATTAATTGGTGCGTTTGCATCTAAATCTGTAAACCCATCAATTATGTATGAAGCAATTGGTCCAACAACAACTGGTGTTTTATTTGGTGTCCAATTAGGAGCGGTTTTATTAGCTGCGGTTTATGGATTTGTATTTACTATTTTATTAGTATATATTACTCGTCCAAGATTATCAGCACGCCAACAATTAGGTCATATAGACTATATTAATCATGGCGAAGATGCATATTCATTCGATATTGAAATTCCATCTGAAAAAGAAATGGAGCAATATGAATTAGCAGATAGTACATGA", "aa": "MGGVTGSLLIGAFASKSVNPSIMYEAIGPTTTGVLFGVQLGAVLLAAVYGFVFTILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYELADST", "aa_hexdigest": "186eb1fd867902a06997d4c47e2b15ec", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11346.833299999995, "isoelectric_point": 4.562602424621583 }, "id": "GPKLONFDEH_459", "locus": "GCA000006625_00426" }, { "type": "cds", "sequence": "contig_1", "start": 262411, "stop": 262614, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCCGCAGATGCAAGTCAATCAACACTTAATGCAATAGGTTTTAATCCTACTAATACACTACTAGTAGCTTTAGCAATTGCTTTTGTTTTACTAATGACACCAGGGTTAGCATTATTTTATGGTGGTTTAGTAAGACGTAAATCAGCTTTAACAATTATTAATCAATGTGTAGCTTCACTTGGAATTACAACATTGATTTGA", "aa": "MPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGGLVRRKSALTIINQCVASLGITTLI", "aa_hexdigest": "12074291a7c50eb8309489cc66b713b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6962.2451999999985, "isoelectric_point": 9.301901435852049 }, "id": "GPKLONFDEH_460", "locus": "GCA000006625_00427" }, { "type": "cds", "sequence": "contig_1", "start": 263023, "stop": 263118, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGAAAACGTACAACATTAATTACAGATAAAAAGCGAGCTAATAATTTACCAATGGTTGTATTAGGTGCAGCATTACTATTTTTTGGTTGA", "aa": "MLGKRTTLITDKKRANNLPMVVLGAALLFFG", "aa_hexdigest": "5c323901354bd9707921ba57a4fe3b20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3390.1155000000003, "isoelectric_point": 11.097023582458494 }, "id": "GPKLONFDEH_461", "locus": "GCA000006625_00428" }, { "type": "cds", "sequence": "contig_1", "start": 263166, "stop": 263372, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATAGGAACAGAGGCTCAAACTGGAACAAAACCTGCTGCGGGAGTAATTGTTGCTAATCCAGCGACTGCACCAGTCACTAAACCTACTGTTGTTGGTTTGTGGTTTTTGTTAACAATAGTTTCAAGTGTAACTCAACCTATCATACCTATTGCTAAGGCAATAATTGTATTTAATCATGATGATATAGCTTGTGAAATGTGA", "aa": "MAIGTEAQTGTKPAAGVIVANPATAPVTKPTVVGLWFLLTIVSSVTQPIIPIAKAIIVFNHDDIACEM", "aa_hexdigest": "4c76495ed011e12787d313652241528a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7014.230699999999, "isoelectric_point": 5.464580345153809 }, "id": "GPKLONFDEH_462", "locus": "GCA000006625_00429" }, { "type": "cds", "sequence": "contig_1", "start": 263374, "stop": 263793, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTTTTAGTATGTTATTCAGTTTCTAGATTATTAGATTACTTCCGTTTTGATGATTCTTTAGAAGTTCTACCAGTTCATGGAATGGGTGGTGTTATAGGTTCTTTATTAATTGGAGCATTTGCTACTAACACGGTTGCACATGGAATTAAATATGACGCCGTCGGATTAGATAATAATGGAAATCCAATTTATGGTATTTTATTTGGACTACAATTAGGTGCTGTATTATTAGCAGCAACATGGGCATTTGCCTTTACATGTTTAATAGTTTATATTACTAAACCTCGTTTATCAGTGCGTCAACAATTAGGCCATATAGACTACATCAATCATGGTGAAGATGCATACAAGTTTGATTTTATTATTCCTTCAGAAAGTGAAATGTTTGAATATGAAAGTTTTGATCAAACTTGA", "aa": "MGVLVCYSVSRLLDYFRFDDSLEVLPVHGMGGVIGSLLIGAFATNTVAHGIKYDAVGLDNNGNPIYGILFGLQLGAVLLAATWAFAFTCLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFDQT", "aa_hexdigest": "9f710d3c9c266d8d45c6136afcff77f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15379.43589999998, "isoelectric_point": 4.652578544616699 }, "id": "GPKLONFDEH_463", "locus": "GCA000006625_00430" }, { "type": "cds", "sequence": "contig_1", "start": 264020, "stop": 264598, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E492BA6", "UniRef:UniRef90_UPI001E492BA6" ], "nt": "ATGCCACGAATAAATACGCAAGTTTTTAATGAAAATAATGATAAAAATAGAATTAAAAGTAATAACAATAAAATTTGTGATGATATTGAATACACTCGCTTATTAGAAACGAAAGAATATAATGCTGGTTTTTCTCCTAATAATGATTCACAAGATATCTTAATTAAAAAACAAGACAATATTTTAAAGTTAAAACCAACTCTAAATTTTGATGATAGTGATCAATTAATTTCAATTGTTCCTACTAAAAATTATGAAAATTTAAGATTAAAAGAAATTGAAATCGATAAAAAAAACACTGTTGATTATCAATTAAAAAAAGAAATAATAGATCAGGACTTAACAGATGATAATCATTTATCTATAAGTCAAAAATCCAATGGTTTTGCTTTAAAAACTAAACAATTTATTCCTCAAAATATTTCAGAATTTATTTATCATAATGAAGACGATCCAAAAACATACGGAAGTGAAAATAATTTAACTTTTAGTCAACTAGAAGAAAAAGTTAATCAAAAAAATACACCTGATTTTTATTTAAAAGAAATTCATGCAGTTCGTAACAAAATTATCATTTGA", "aa": "MPRINTQVFNENNDKNRIKSNNNKICDDIEYTRLLETKEYNAGFSPNNDSQDILIKKQDNILKLKPTLNFDDSDQLISIVPTKNYENLRLKEIEIDKKNTVDYQLKKEIIDQDLTDDNHLSISQKSNGFALKTKQFIPQNISEFIYHNEDDPKTYGSENNLTFSQLEEKVNQKNTPDFYLKEIHAVRNKIII", "aa_hexdigest": "ab6c3d316eaf2ede2c92efc5a34a7d5c", "start_type": "ATG", "rbs_motif": "GGxGG", "psc": { "uniref90_id": "UniRef90_UPI001E492BA6", "query_cov": 1.0, "subject_cov": 0.803347280334728, "identity": 1.0, "score": 299.0, "evalue": 1.18e-100, "valid": true, "uniref50_id": "UniRef50_UPI001E492BA6" }, "pscc": { "uniref50_id": "UniRef50_UPI001E492BA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E492BA6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22541.922800000008, "isoelectric_point": 5.325950050354003 }, "id": "GPKLONFDEH_464", "locus": "GCA000006625_00431" }, { "type": "cds", "sequence": "contig_1", "start": 264638, "stop": 264862, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTATGAAGTTATTATTAATCATTTTAATCCATGGATTTTTTTACCAATTAGTATTTATTGTGTCTTGATGATTTTCTTTTTAGCTTTATCATGTAGTAATTTTATAAATTTTAAAAAAGAAATGGATTATCAAAAAAACCATTTTGATTGTACAAAAGCAACAAATTTTATTGTAGGTATATATCGTAAATTAATAGTAGTACATATTAATGTAAACTGA", "aa": "MIYEVIINHFNPWIFLPISIYCVLMIFFLALSCSNFINFKKEMDYQKNHFDCTKATNFIVGIYRKLIVVHINVN", "aa_hexdigest": "9c9ad2ba479c620605fde1c00c4e8edb", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8850.485399999998, "isoelectric_point": 8.52151165008545 }, "id": "GPKLONFDEH_465", "locus": "GCA000006625_00432" }, { "type": "cds", "sequence": "contig_1", "start": 264896, "stop": 265306, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTTGGTGTTTTTGTTGTCAGTTATTTTATGAATTTATATTATAATGCAGCTATAGGAAGTCGTTTTGGTGATTTGATTATAAAAATTCCTGTTGTTATTAATAATCATCTTTCACAAAAAATTAGTTTTAACAAAAATCCATTATGGGCAATGATTGGATTAGGTGCTATTTTAGGATTAACATTTATTATTCATTTATGGTTGGTTTTTAATGCTCATCATCGTATTAATCGTATTCATAGTTATTATGTTAATGAAATCATTACTAGTGAAGAAATAATTTCATTAAAAAAACAAGCAAATCGTCGTGGTCTAGTCATATTTTTAGTGTTAACTGTTATTTTAGGTTTAATTTTTTATTTGATGTATACTATTTTAAAAAGAAAATACACTATCAAAAAATAA", "aa": "MIFGVFVVSYFMNLYYNAAIGSRFGDLIIKIPVVINNHLSQKISFNKNPLWAMIGLGAILGLTFIIHLWLVFNAHHRINRIHSYYVNEIITSEEIISLKKQANRRGLVIFLVLTVILGLIFYLMYTILKRKYTIKK", "aa_hexdigest": "d4b97649a4d75cc7c829e021c713928d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15817.869299999995, "isoelectric_point": 10.14630832672119 }, "id": "GPKLONFDEH_466", "locus": "GCA000006625_00433" }, { "type": "cds", "sequence": "contig_1", "start": 265321, "stop": 266886, "strand": "+", "frame": 1, "gene": "fhs", "product": "Formate--tetrahydrofolate ligase", "db_xrefs": [ "COG:COG2759", "COG:F", "EC:6.3.4.3", "GO:0004329", "GO:0005524", "GO:0035999", "RefSeq:WP_006688872.1", "SO:0001217", "UniParc:UPI00000C1BD6", "UniRef:UniRef100_Q9PQS1", "UniRef:UniRef50_Q9PQS1", "UniRef:UniRef90_Q9PQS1" ], "nt": "ATGGCAAAAATTATTGAAGAAATGATTAATAAAAAAGGGTTGTTAATTGATGATTATGAACTTTATGGCAATGAGATTGCTAAAATTAAATCAATTAAAAAACTAAATAAAAAGGATTCAAAATTAATTGTTATTACCTCAATGAATCCTAATCCAGCTGGTGAAGGTAAAACAACAACTGCTATTGGTTTAGTTGATGCTTTAAATAAACATGGATACAAAGCAATTGGAGCCTTACGAGAGCCATCAATGGGACCAGTTTTTGGTATGAAAGGTACGGGTTCTGGAGGAGGATTATCTTTTTTAAAACCCTTTGATAAAATTAATTTACATTTTAGTGGTGATTTCCATGCGATTACTGCAGCTAATAATTTAATCGTTGCAGTAATAGAAAATGAAATACATAATAGGTCTTCAATGCAAATCGATTCTCAAAAAATTTTAATTAAAAGATGTCTAGATGTTAATGATCGTTCTTTACGTAATATTGAATATGATATTAACCATCAACAAACAAAAAGTGGTTTCAACATTACAGCAGCGAGTGATTTAATGGCATTATTTTGTTTAGCACATGATCATAAAGATTTTGAAGATAAACTTGCGCAAACAATTGTTGCATATAATATAGTAAACCAACCTATAAGAATTTGTGATTTAGAATTAACAAAAGCTATTATGGCAATTTTAGAAGATGCTTTATATGCTAATTTAGTACGAACTAATGAGGATAACCCTGTTTTTGTTCATGGTGGCCCATTTGCTAATATTGCTCATGGTTGTAATAGTATTATTGCAACCAAAAATGCTTTAGCTTTAGGTGATTATGTTGTTACTGAATGTGGTTTTGGTAGCGATTTAGGACTTGAAAAATTTATGAATATCAAGATGGCTAGTTTGAATTTAAAACCAGATTTAATTGGTTTAGTTATTAGTCTAAAATCAATCGCATATCATGCTCAAACAAACGAAAAAGACTACGTTAAACAAGGCTTTGCTAATGTTTTATGTCATATTAATCACATTAAAAAATATAATGTTTCTTTTATTGTTTATATTAATGTCAATACTAATACTGATAGCGAAGAAGACTTATTAACTTTAGAAAAACTATTAGATGAACATCAAATTGAACATGCACGTTCTTATGCTTATAGCTATGGTTCTAAAAAAAGTGAAGAAATAACTAAAAAAACAATTACTTTAACTAATCAAATTAATGATCATGAATTAAAATTAATTTATGATATTAAAGATCATTTATCATATAAATTAAAAAAAATATGTGAAAATGTTTATGGTGCTGATGGATATGAATTAAGCTATGAAGCTAAAGAACAATTGAATCGTTATGAACATTTAGATTTTTATTTATGCATTGCTAAAACACCATATTCAATTTCTGATGATGCAAAATTATTAAATAATCCTAAGAACTTTAAAATTCATATTGAACGCTTTGAAATTAATTATGCTGCAAAATTAATCATTGCTATTACAACAACTATTTATCGTATGCCTGGCTTAAATAAAGAACCAGCGGCCAAAAATTTCGTTATGAAATAA", "aa": "MAKIIEEMINKKGLLIDDYELYGNEIAKIKSIKKLNKKDSKLIVITSMNPNPAGEGKTTTAIGLVDALNKHGYKAIGALREPSMGPVFGMKGTGSGGGLSFLKPFDKINLHFSGDFHAITAANNLIVAVIENEIHNRSSMQIDSQKILIKRCLDVNDRSLRNIEYDINHQQTKSGFNITAASDLMALFCLAHDHKDFEDKLAQTIVAYNIVNQPIRICDLELTKAIMAILEDALYANLVRTNEDNPVFVHGGPFANIAHGCNSIIATKNALALGDYVVTECGFGSDLGLEKFMNIKMASLNLKPDLIGLVISLKSIAYHAQTNEKDYVKQGFANVLCHINHIKKYNVSFIVYINVNTNTDSEEDLLTLEKLLDEHQIEHARSYAYSYGSKKSEEITKKTITLTNQINDHELKLIYDIKDHLSYKLKKICENVYGADGYELSYEAKEQLNRYEHLDFYLCIAKTPYSISDDAKLLNNPKNFKIHIERFEINYAAKLIIAITTTIYRMPGLNKEPAAKNFVMK", "aa_hexdigest": "95554c84b10f5fa38bc8255937f3592f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00000C1BD6", "ncbi_nrp_id": "WP_006688872.1", "uniref100_id": "UniRef100_Q9PQS1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BD6", "RefSeq:WP_006688872.1", "UniRef:UniRef100_Q9PQS1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQS1", "uniref90_id": "UniRef90_Q9PQS1" }, "psc": { "uniref90_id": "UniRef90_Q9PQS1", "gene": "fhs", "product": "Formate--tetrahydrofolate ligase", "ec_ids": [ "6.3.4.3" ], "uniref50_id": "UniRef50_Q9PQS1", "cog_id": "COG2759", "cog_category": "F", "go_ids": [ "GO:0005524", "GO:0004329", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQS1" ], "product": "Formate--tetrahydrofolate ligase" }, "genes": [ "fhs" ], "id": "GPKLONFDEH_467", "locus": "GCA000006625_00434" }, { "type": "cds", "sequence": "contig_1", "start": 267016, "stop": 267120, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATTTTATTAGATATACTAAAAAAAGAAATTTAAGTCTTAACACAATTCGTACTTATGAATCAGTATTAAAACATTATGAACCTGTTTTAGATTCGTGA", "aa": "MKDFIRYTKKRNLSLNTIRTYESVLKHYEPVLDS", "aa_hexdigest": "62b6a9d856ff04c57deeaea2ea449583", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4159.7647000000015, "isoelectric_point": 9.599294090270995 }, "id": "GPKLONFDEH_468", "locus": "GCA000006625_00435" }, { "type": "cds", "sequence": "contig_1", "start": 267268, "stop": 267768, "strand": "+", "frame": 1, "gene": null, "product": "site-specific integrase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00130DC0CA" ], "nt": "ATGAAATATTTTGATGTAATTAGTAAAAATAATTTATATAAAAAAACTGATATCTTAGATGATGATTCATTAGAAATTAAAAAATATAAAACCATTATTAGATTCTTGTTTGAAACAGGAATTAGAGCTCATGAATTATTTTTTCTAGAATCTATTAATAATCGTTTGTATGTGTTAGGTAAAGGAAATAAAAAACGACAAATTTTCTTTGTAAAACAAACTTTTGAACAATTACAAAAGTTTTATGAGAATCTAAAAGGATTTGAAACAACTAAAACATTACGTTTATATATTAAAAAAATTATTGGCAAGAATTTTACTCCCCATTCATTACGTCGTAGCTTTGCTACTTTTATGTTAATTAAAGGGGCAAATCCCAAAACTGTAATGTTACAAATGGGACATGCAAACATTCAAACAACGTTTAGTTATTTAAATTTAAATGAACAAACAAATCGTCGAATTTATAACAAAATTATGTACCAAAATGATGCAGAATAA", "aa": "MKYFDVISKNNLYKKTDILDDDSLEIKKYKTIIRFLFETGIRAHELFFLESINNRLYVLGKGNKKRQIFFVKQTFEQLQKFYENLKGFETTKTLRLYIKKIIGKNFTPHSLRRSFATFMLIKGANPKTVMLQMGHANIQTTFSYLNLNEQTNRRIYNKIMYQNDAE", "aa_hexdigest": "63f743b4fdfb1e456d1f91ec351113e6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00130DC0CA", "query_cov": 0.9337349397590361, "subject_cov": 0.9393939393939394, "identity": 0.516, "score": 139.0, "evalue": 4.28e-39, "valid": false, "product": "site-specific integrase", "uniref50_id": "UniRef50_UPI00130DC0CA" }, "pscc": { "uniref50_id": "UniRef50_UPI00130DC0CA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00130DC0CA" ], "product": "site-specific integrase" }, "genes": [], "id": "GPKLONFDEH_469", "locus": "GCA000006625_00436" }, { "type": "cds", "sequence": "contig_1", "start": 267911, "stop": 268123, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGTAAGCGATTTATCAAAAATAAAATTAGTTTCAAATATTTTTAGAATAAGAGGTGATAAAAAATTGATAAATAATAAAATAAAAGAAGCAAAAATCAAAAAGAAACTAGAAAAAAACAAAAAAGTTTCTTTAATGGGTTTTGTTTTACTAACAGCTTCAGCAATTATTGCTTTTTATACATTCCCATCACTTTCAACAGCTGGTTGA", "aa": "MVVSDLSKIKLVSNIFRIRGDKKLINNKIKEAKIKKKLEKNKKVSLMGFVLLTASAIIAFYTFPSLSTAG", "aa_hexdigest": "c89efec22412d32057151bd3cd424f59", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7798.3881999999985, "isoelectric_point": 10.390707969665527 }, "id": "GPKLONFDEH_470", "locus": "GCA000006625_00437" }, { "type": "cds", "sequence": "contig_1", "start": 268316, "stop": 269287, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00324.27" ], "nt": "ATGATCTTATTCGTCCTTTCATCCTTTTCATTTGCTTTCGCAGGTGCTGATGGATATAATTATTTTAATACACTTAAGTTGGGAACAACTTTAAATCAAGCTACATCAGTCAACTTTTCAAAAAACTTTAGCAATGGGGCATTATATGGAATTGGTATTGTTGTTGTCGCAGGTCTAATCTTTATGAGTTTATTAGGCCAAAAACGAACTCACCAATTTGGCCAAGTTGCTTTAATCATTGGTATTTTACTACCATTCTTTATTATTTTAGGATTCCAAATTTATACAGTGGCAACAATGCCAGATCCATTATACTTTATTGGAAAACACTTCCAAAAAGAACCACATTTAGACCCAAGTGTAACTGGTACTTCTTATGAATTAAATAAAACTTTCATGTCATCAGTTGTTATGGCATCTTTCATGGCATTTAGTTTTAGCTTACATGGTGTAGAAGTTTCAGCTATTGCTGCTAATCGAATGGATAATCCTTCTAAGAAATATCCACAAGCCATGATGATTGTTGTAATTTCTGCTTTAGTTTGCATTATTATTGGATCAATTATGATTTCAATGACTGTACCAACTTCAACATTATCATTCAATGGTGGATTAGTACAGACAATTATGTTCAACTTATCTGTTGGTGGAATTGATGTTAAACTACCAGATGGCACAGTTGCTCATTATAAAACACTAGCACATGCATACTCAGTAATTGAAACTCAATATGGCACAGCTGCCGCTAACCAAGTTATGTATCAATTTGTGTATGGTCATCCAATTAATACAGGTAGTGACTTAGCACGTTCTGATTTATTAACACTAACATCAGAAGTTAACGATCCTTTAAAAAACCCATCTTTATTTATTTCTGTTATGAATATAGAAACAAATAAAATGGCTATGCAAGGATTACAAGCTATTACTTTCTTCATTGGAATAGGTGTATTTGTTGAAGTTTCTGTTTGA", "aa": "MILFVLSSFSFAFAGADGYNYFNTLKLGTTLNQATSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTHQFGQVALIIGILLPFFIILGFQIYTVATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVEVSAIAANRMDNPSKKYPQAMMIVVISALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKLPDGTVAHYKTLAHAYSVIETQYGTAAANQVMYQFVYGHPINTGSDLARSDLLTLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITFFIGIGVFVEVSV", "aa_hexdigest": "bce6d07e0e0494368617009285585273", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00324.27", "name": "AA_permease", "length": 179, "aa_cov": 0.5325077399380805, "hmm_cov": 0.9217877094972067, "evalue": 5.320213930997961e-12, "score": 40.68571853637695, "start": 37, "stop": 208 } ], "seq_stats": { "molecular_weight": 35012.48280000001, "isoelectric_point": 6.6421705245971685 }, "id": "GPKLONFDEH_471", "locus": "GCA000006625_00438" }, { "type": "cds", "sequence": "contig_1", "start": 269288, "stop": 269398, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAACCTTTCAACTGGATTAAATTATGCAATGCGTAAAGCCCACTTCCCAGCATGGGTTACATATCGTTTAAAGAATGGGTCAGCGTTAACAGTAGCAATTTTATGA", "aa": "MSNLSTGLNYAMRKAHFPAWVTYRLKNGSALTVAIL", "aa_hexdigest": "127f57d2d1653575b61a5fb19123d1d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3996.6589999999997, "isoelectric_point": 10.446408653259276 }, "id": "GPKLONFDEH_472", "locus": "GCA000006625_00439" }, { "type": "cds", "sequence": "contig_1", "start": 269411, "stop": 269842, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGCATTTTTGCAATTTACATGTTTGGTTATAATTCATTAATGCAATACAAAGGTCAAACAATTAGTGATATTTTAGCTAATGAAATTCGTATTAACCAAGATCAAAAAGAACCAATGGCTTTATTGTCTAATCAAAATTTAGATGAAATTAAACTAATGGCTCGTTCAATTTACGAACATAGTCATGCTCCTGGAGCACAAGCTAATGTTTTACATTTTGATTTTAATAAACATGCTAACAGTGAAGTAAAAGCAATTTATGATAAAATCAAAGATTATATTGGCATTAATGCCGTAGCATCGGGAACACCAGCATCAATTACTAATATTTCATTCATTTCAAACGTTGTTTCACAAATCTCAATGTATTATATTGGATATAACATTTTCTTAATTGGATATCTAAGATATGTCTTTAAAGCTAATTGA", "aa": "MCIFAIYMFGYNSLMQYKGQTISDILANEIRINQDQKEPMALLSNQNLDEIKLMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINAVASGTPASITNISFISNVVSQISMYYIGYNIFLIGYLRYVFKAN", "aa_hexdigest": "3c284fb5d529845197657797c0a292e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16218.436799999987, "isoelectric_point": 6.893739891052246 }, "id": "GPKLONFDEH_473", "locus": "GCA000006625_00440" }, { "type": "cds", "sequence": "contig_1", "start": 269903, "stop": 270064, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGTTAATACATTTGCAGTTGTTGCAACTTACTTACCAGCTGCTCCAGAACTATATCCTGGACAAGAAGCTTATTATGTATTTATTTCAATTGCATTACCAATGTTTATATGCACATTATTACTTGGATTATTTATTTATCTAATTAATTATGCTTGA", "aa": "MGVNTFAVVATYLPAAPELYPGQEAYYVFISIALPMFICTLLLGLFIYLINYA", "aa_hexdigest": "55385c5ff3ffba4801ee6e649608e269", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5880.968799999997, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_474", "locus": "GCA000006625_00441" }, { "type": "cds", "sequence": "contig_1", "start": 270194, "stop": 270328, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATTATTACAACAAAAACAAGTAATTGTAAGAACAACAAAAGATAAGGCACAAAGAGAATTAGTTGCGAATGAAATGCAATATTTAGCAGCTGAAATCGCAACAAGATTTGCACCAGAAGAAGAACATTAA", "aa": "MQLLQQKQVIVRTTKDKAQRELVANEMQYLAAEIATRFAPEEEH", "aa_hexdigest": "fae5e00c3097670d40cadff766e53ee0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5156.849699999999, "isoelectric_point": 5.668802833557129 }, "id": "GPKLONFDEH_475", "locus": "GCA000006625_00442" }, { "type": "cds", "sequence": "contig_1", "start": 270536, "stop": 271654, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T368", "UniRef:UniRef90_A0AAC9T368" ], "nt": "ATGACAACGCCAATTTTATTAATTGGCGGATTATCAACATTGGCATTAACAAGTTGTAAAGACGGAAAAAAATCTAATCCAAAACCAGATAAACCATCAACTATAGAAGAATATAAATTAAGTTTATCAAGTGTTGAAACTGGTTTTAATGAAGCTAAATTAATTTTTGATAATAATGATAGTTTTAAAACATTAGATAATTTTAAAGTTATTTTAAAAAATACTCAAAACACTAATGAAGTTATTGAAGCAAGTAATTTATCTGCAACAATTGAAAATCAAAAATTAGTTTTAAAACTAAATAATTTACAAGACGGTCAAACATATAAAATTAACAAAATTTTATATCAAACAAATAAAGAAGTTATTTTAAGTAATAATCAAGTATTTACTACAAAAACTAAACCTAAAATTGAATATGAAGTACAAGAAAAAATTTTGAAACTTGATGATAAAACATATTATGTTGTTCTAAAAGTTAAAGACCCTCAAAATTTAAATGAACCAACAAAAGAAATTTTAAAATCTGTAACTTTTACTTCAAAAATCTCAAATGTTGCAAATCTTAAAAATGCAATATTAGAAAATCAAAGACCTTACGTTAAAGAAGATTATTCAGAAATTCAAATTAAATTACCAAGAGTACCTGTAATTAGTGAAAAAATAGAAATCACTGCAAACAATTCCTTATTTAAATCATTCACAATTACTGTTGGTGAAAAACATATTAAAAATGTGGAAAAAATCATCGATATCACTAAAAAAGTTTTTGGTAATCAAGAATTAAAATCTATTAGTATTACAAAACAAAATAGTAGCAGTGCCAATATAACATTAAGTTTAAAATTAAATGGTAATTCAATTATTGTTGGTGCTAACAAATTAATTAATAAAAAAGACAAAGCAATAAAATTTAGCTTCTTTGTTAAAAATAAAGCAACAAATACAGTTAAAGAATTTAATTTCAATGAAAAATCAAAACCTATTGGAAGCTATCAATTCACATTAAATGGATTAGATGCTAATAGTGATTTTGTAATTGAAGATATTAGATATGATGATAAATCAATCAAAATTACAGAAGAGCTTTTAGCTAGCTTAAGTTTTTCAACAAAATAA", "aa": "MTTPILLIGGLSTLALTSCKDGKKSNPKPDKPSTIEEYKLSLSSVETGFNEAKLIFDNNDSFKTLDNFKVILKNTQNTNEVIEASNLSATIENQKLVLKLNNLQDGQTYKINKILYQTNKEVILSNNQVFTTKTKPKIEYEVQEKILKLDDKTYYVVLKVKDPQNLNEPTKEILKSVTFTSKISNVANLKNAILENQRPYVKEDYSEIQIKLPRVPVISEKIEITANNSLFKSFTITVGEKHIKNVEKIIDITKKVFGNQELKSISITKQNSSSANITLSLKLNGNSIIVGANKLINKKDKAIKFSFFVKNKATNTVKEFNFNEKSKPIGSYQFTLNGLDANSDFVIEDIRYDDKSIKITEELLASLSFSTK", "aa_hexdigest": "feab9ca6521cfc8157023ba1bf6928d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAC9T368", "query_cov": 1.0, "subject_cov": 0.9738219895287958, "identity": 0.97, "score": 677.0, "evalue": 7.52e-245, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T368" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T368", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T368" ], "product": "Lipoprotein" }, "genes": [], "id": "GPKLONFDEH_476", "locus": "GCA000006625_00443" }, { "type": "cds", "sequence": "contig_1", "start": 271873, "stop": 272028, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B3", "UniRef:UniRef90_A0AAC9T0B3" ], "nt": "ATGCCAGCACTTAAAGAAGCTTTTAGTACTTTGAAAGAAGAAATTAATAAACAAATCAATAAGAGTGAAAAATTATCTGATGAAGCGTATAAAGCATTAACAGCTTCATTAAAAGTTTCAATTGATGGAATGAAAAAAACATTAGGACAAAATTAA", "aa": "MPALKEAFSTLKEEINKQINKSEKLSDEAYKALTASLKVSIDGMKKTLGQN", "aa_hexdigest": "260e8b35733bf59b046b60fd59987efc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 271711, "stop": 272028, "observations": { "mutation": [ 271720, 271849 ], "stop": [ 271720, 271849 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0B3", "query_cov": 1.0, "subject_cov": 0.4811320754716981, "identity": 0.961, "score": 92.0, "evalue": 4.19e-23, "gene_start": 1, "gene_end": 51, "reference_start": 55, "reference_end": 105, "reference_sequence": "MKKWQKILTIALPTSLLVAIPIVVASCSTKSEAQKEFEKTYKEYLSLLDKLASKMPALKEAFSTLKEEINKQINKSEKLSDEAYKTLTTSLKVSIDGMKKTLGQNC" }, "paralog": false, "identity": 0.9619047619047619, "score": 189.0, "evalue": 4.69e-64, "description": "Internal stop codon at 271720, 271849. Nonsense mutation around 271720, 271849.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0B3", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T0B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0B3", "UniRef:UniRef50_A0AAC9T0B3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B3" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_477", "locus": "GCA000006625_00444" }, { "type": "cds", "sequence": "contig_1", "start": 272239, "stop": 272481, "strand": "+", "frame": 1, "gene": null, "product": "Unique hypothetical membrane lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQR6", "UniRef:UniRef90_Q9PQR6" ], "nt": "ATGTATAATGCTGGAGTGGCTTTTTTAGTAATTTTTGCTTTAATTGAAATTGCTAGTTTTGCTTTATTTGTAACAACAACAGTCAATGTTGGTTTAAATTTAAAAAGACAAGAAAACTTTGTCTTGCCATTTGTTTTTGGCATTTTAGGGTGCTTAGGAATAATACCTATAATCGGATCAATTATTATTTATGTACAATTAAATAAATTTTATAAGCAACAAAACGAATCTAGTTTAATATAA", "aa": "MYNAGVAFLVIFALIEIASFALFVTTTVNVGLNLKRQENFVLPFVFGILGCLGIIPIIGSIIIYVQLNKFYKQQNESSLI", "aa_hexdigest": "78d3eb77bfa286256045fae4c3054dad", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 272143, "stop": 272481, "observations": { "mutation": [ 272233 ], "stop": [ 272233 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQR6", "query_cov": 1.0, "subject_cov": 0.7142857142857143, "identity": 1.0, "score": 151.0, "evalue": 4.11e-46, "gene_start": 1, "gene_end": 80, "reference_start": 33, "reference_end": 112, "reference_sequence": "MHLGIYYMVYSYYGSPYLTQDIATTNVVNAWAMYNAGVAFLVIFALIEIASFALFVTTTVNVGLNLKRQENFVLPFVFGILGCLGIIPIIGSIIIYVQLNKFYKQQNESSLI" }, "paralog": false, "identity": 0.9821428571428571, "score": 217.0, "evalue": 1.85e-74, "description": "Internal stop codon at 272233. Nonsense mutation around 272233.", "psc": { "uniref90_id": "UniRef90_Q9PQR6", "product": "Unique hypothetical membrane lipoprotein", "uniref50_id": "UniRef50_Q9PQR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQR6", "UniRef:UniRef50_Q9PQR6" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQR6" ], "product": "Unique hypothetical membrane lipoprotein" } }, "id": "GPKLONFDEH_478", "locus": "GCA000006625_00445" }, { "type": "cds", "sequence": "contig_1", "start": 272656, "stop": 273189, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAAATTAAATTAAATAAAAAAATTATTTTAGCATCAGTTATTGGAATAATGGGTGTAGCTTTGGTTCCTATAGCATTGGTTGCATGTAGTCAAAATAAAACAAAATCTGATTCTAGAAACTTAACTTTAAACAATTTTTATGGTCGCCCATCAAGTGATGGGGATGATGCATATCAAGCAATCTATGCGTCTAAAATTCAAGATGGTGCACGTATGTTAGGATTAATTTCATTTAGACATAAAAATCCAATTTCAAAATACTTTAATTCACCAAAAGATAATCAACAAGTTTCAGCAGTATTAATTGATGAAATTTATGATTTACAAACAGGTAAAGATCGAATTGCATCAATCACATATCGTGCAGATCAAGCTGCTTTTTTAGCTGGAATTGCTGCAGCTTATTATTTAAATAGTAATCAAAATGTTTTTGGTAAAGATAATAAATTAACATGGGGCGGTTTTGTTGGTATTCATTTACCATCAACTACAAGATTTATTCAAGGTTTTAAATTTGGTATTCAATGA", "aa": "MRKIKLNKKIILASVIGIMGVALVPIALVACSQNKTKSDSRNLTLNNFYGRPSSDGDDAYQAIYASKIQDGARMLGLISFRHKNPISKYFNSPKDNQQVSAVLIDEIYDLQTGKDRIASITYRADQAAFLAGIAAAYYLNSNQNVFGKDNKLTWGGFVGIHLPSTTRFIQGFKFGIQ", "aa_hexdigest": "6617669230e6b2ccab790585dad83a0f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19528.200099999984, "isoelectric_point": 9.765622520446776 }, "id": "GPKLONFDEH_479", "locus": "GCA000006625_00446" }, { "type": "cds", "sequence": "contig_1", "start": 273373, "stop": 273882, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTGGACCTCAAATCGATTATGCAACAACAGCTGCTGCTGAATCAAGTAAACCAATCGTTGTTGTTGGTGTTGATACAGAACAAGAATTAGATGATAATACAAATAAAGCACGTATTAGTGAAAATAATAAATCATTAGCTAACGGTAAAACAATTATTTTTTCTATTGTTAAACGTTTAGATTTAGCTTTTAAAGGTGCTTTATTAAAAGCATCAGAAGGGGCACAATTAACTAATGATATTAACAAAGACGCTTATAAATTAGGAACACACACTGAAGCAAGTTTTAATAAAAATACTTATGTTGATAATACAGCGTTAGTTGAATTATCAAAAGCTGGACATCAGTATTTAATTGATGCAATTAAATTAAGTGGTTTAAAAGAAGTAAATGATTATAAAACAATTGTTGAAATAATCCAAGAAGATCCTTTATTTAAACTTTTATCACAAATTGGAACTAAAAAATTAGATGAAGTTGCTACTAAATCACAACAAGGTGATTGA", "aa": "MAGPQIDYATTAAAESSKPIVVVGVDTEQELDDNTNKARISENNKSLANGKTIIFSIVKRLDLAFKGALLKASEGAQLTNDINKDAYKLGTHTEASFNKNTYVDNTALVELSKAGHQYLIDAIKLSGLKEVNDYKTIVEIIQEDPLFKLLSQIGTKKLDEVATKSQQGD", "aa_hexdigest": "e825a43294b277304cac6a4d2cac82cd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18372.498199999987, "isoelectric_point": 5.269963645935058 }, "id": "GPKLONFDEH_480", "locus": "GCA000006625_00447" }, { "type": "cds", "sequence": "contig_1", "start": 273931, "stop": 274056, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTGGATTAGTTTATGTTGATCAAAAAAATGAACTATATCCATATGAATTATCTAATTCATTTTATTTAGAAAAAGATCCAAATAAACGTCAAGCTTCAAGAGCTTTTTATAATTATTGA", "aa": "MLGGLVYVDQKNELYPYELSNSFYLEKDPNKRQASRAFYNY", "aa_hexdigest": "3b87588ec6e890487aa5a8bdc36128a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4925.442700000002, "isoelectric_point": 6.037346839904784 }, "id": "GPKLONFDEH_481", "locus": "GCA000006625_00448" }, { "type": "cds", "sequence": "contig_1", "start": 274407, "stop": 274781, "strand": "+", "frame": 3, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022", "SO:0001217", "UniRef:UniRef50_Q9PQR4", "UniRef:UniRef90_Q9PQR4" ], "nt": "ATGAATACACCAAACAATCAACGCCATATGTCATCAAATAATGATGCACGTAAAAATCAACCTTTAATTAATGATCAAATTCGTTTTCGAACAATGGTTGTAATTGATGACCATGGTAATAATTTAGGTGAAATGAATCGTATTGATGCCCTTAATTTAGCCGCTTCAAAGAATTTAGATTTAGTTGTTATTGCTAAAAAAGGAAATATTCCTGTAACAAAAATTTTAGATTATGGTAAGTACAAATACGAACAAAAACGTCGTCAAAAAGAATCAAGAAAAAATCAAACAATTATTAAGGTAAAAGAAATCAAAATTAAACCAATGATTGGTGAACATGATTTAAAAGTAAGAGCAGAAAATGCTAAACGTTGA", "aa": "MNTPNNQRHMSSNNDARKNQPLINDQIRFRTMVVIDDHGNNLGEMNRIDALNLAASKNLDLVVIAKKGNIPVTKILDYGKYKYEQKRRQKESRKNQTIIKVKEIKIKPMIGEHDLKVRAENAKR", "aa_hexdigest": "53a4b9940c46664ac6d058ca6b19f983", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "infC" ], "truncated": "3-prime", "pseudogene": { "start": 274407, "stop": 274970, "observations": { "mutation": [ 274779 ], "stop": [ 274779 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQR4", "query_cov": 1.0, "subject_cov": 0.6631016042780749, "identity": 0.992, "score": 235.0, "evalue": 2.45e-77, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MNTPNNQRHMSSNNDARKNQPLINDQIRFRTMVVIDDHGNNLGEMNRIDALNLATSKNLDLVVIAKKGNIPVTKILDYGKYKYEQKRRQKESRKNQTIIKVKEIKIKPMIGEHDLKVRAENAKRWLEDKDNVKFVIEARGRMCTKDEFILQAYEKFIDLIKDYGTVVQANKKVSNYRYETIIEPIKK" }, "paralog": false, "identity": 0.983957219251337, "score": 367.0, "evalue": 9.16e-132, "description": "Internal stop codon at 274779. Nonsense mutation around 274779.", "psc": { "uniref90_id": "UniRef90_Q9PQR4", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_Q9PQR4", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQR4", "UniRef:UniRef50_Q9PQR4", "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQR4" ], "product": "Translation initiation factor IF-3" } }, "id": "GPKLONFDEH_482", "locus": "GCA000006625_00449" }, { "type": "cds", "sequence": "contig_1", "start": 274800, "stop": 274970, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATTTGTGATTGAAGCACGTGGACGTATGTGTACAAAAGATGAATTTATTGTACAAGCTTATGAAAAATTCATTGACTTAATTAAAGATTATGGTACAGTTGTTCAAGCTAATAAAAAAGTTAGCAATTATCGATATGAAACAATTATTGAACCAATTAAAAAATAA", "aa": "MKFVIEARGRMCTKDEFIVQAYEKFIDLIKDYGTVVQANKKVSNYRYETIIEPIKK", "aa_hexdigest": "9cc6727a5b8b6fddb7f16a7dea468402", "start_type": "GTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6662.752000000002, "isoelectric_point": 9.130802345275882 }, "id": "GPKLONFDEH_483", "locus": "GCA000006625_00450" }, { "type": "cds", "sequence": "contig_1", "start": 274985, "stop": 275179, "strand": "+", "frame": 2, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02916", "RefSeq:WP_006688895.1", "SO:0001217", "UniParc:UPI000013400C", "UniRef:UniRef100_Q9PQR3", "UniRef:UniRef50_Q9PQR3", "UniRef:UniRef90_Q9PQR3" ], "nt": "ATGGCTAAAATTAGACAAAAAACAAAAAGAGCTGTTGCAAAACGTTTTAGTATTACAAAAAATGGTAAATTAAAACGTAAACACGCTTATAGATCACATTTAGCTCTAGGTCGTTCAACAAAAGCTAAACGTCATTTAAGAAAAGACGCAATTATGAGCACATCTGACACAAAAAGATATACTCAATGCTTATAG", "aa": "MAKIRQKTKRAVAKRFSITKNGKLKRKHAYRSHLALGRSTKAKRHLRKDAIMSTSDTKRYTQCL", "aa_hexdigest": "2c6112c937e09ff084d377167275f808", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000013400C", "ncbi_nrp_id": "WP_006688895.1", "uniref100_id": "UniRef100_Q9PQR3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013400C", "RefSeq:WP_006688895.1", "UniRef:UniRef100_Q9PQR3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQR3", "uniref90_id": "UniRef90_Q9PQR3" }, "psc": { "uniref90_id": "UniRef90_Q9PQR3", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_Q9PQR3", "cog_id": "COG0291", "cog_category": "J", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQR3" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "GPKLONFDEH_484", "locus": "GCA000006625_00451" }, { "type": "cds", "sequence": "contig_1", "start": 275468, "stop": 275596, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCATTAGTAAAAGCTAATGTTGTTACTAAAGATGGTCAAGGTTTAAATAGAAAAATGCTATCTGAATTAGCAATTAACAACCCTGAAGCATTTAATCAATTAGTTGATAAAGTAATGAAATAA", "aa": "MNALVKANVVTKDGQGLNRKMLSELAINNPEAFNQLVDKVMK", "aa_hexdigest": "3a50490a8e7b9bdc4c3131dbc2c6ec2f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4643.412300000002, "isoelectric_point": 9.398732948303223 }, "id": "GPKLONFDEH_485", "locus": "GCA000006625_00452" }, { "type": "cds", "sequence": "contig_1", "start": 275815, "stop": 276120, "strand": "+", "frame": 1, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_004025692.1", "SO:0001217", "UniParc:UPI0000134BD5", "UniRef:UniRef100_Q9PQR1", "UniRef:UniRef50_O52331", "UniRef:UniRef90_Q9PQR1" ], "nt": "ATGAATCAAGAATTAAGAATTAGATTAGAATCTTATGACCACAGATTATTAGATGATACAGTAAAAACAATTGTAAACATTTCTAACTCTACAGGTTCTAAATTAAGAGGTCCAATTCCTCTACCAACTAAAAAGGAAATTTTTACAATTTTAAGATCACCACATGTTAATAAATCAAGTCGTGAACAATTTGAAAGAAGAACACACAAACGTTTAATTATTTTAGAAAATCCACAACCTAAAACAATGGAAGCTTTAAAACGTTTATCAGTTCCATTTGGTGTTGAAGTTACTTTCAAAATTTAA", "aa": "MNQELRIRLESYDHRLLDDTVKTIVNISNSTGSKLRGPIPLPTKKEIFTILRSPHVNKSSREQFERRTHKRLIILENPQPKTMEALKRLSVPFGVEVTFKI", "aa_hexdigest": "41422808dcdb5644ddba279f64f59de0", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000134BD5", "ncbi_nrp_id": "WP_004025692.1", "uniref100_id": "UniRef100_Q9PQR1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134BD5", "RefSeq:WP_004025692.1", "UniRef:UniRef100_Q9PQR1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQR1", "uniref90_id": "UniRef90_Q9PQR1" }, "psc": { "uniref90_id": "UniRef90_Q9PQR1", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_O52331", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_O52331", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52331" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "GPKLONFDEH_486", "locus": "GCA000006625_00453" }, { "type": "cds", "sequence": "contig_1", "start": 276145, "stop": 276846, "strand": "+", "frame": 1, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "RefSeq:WP_006688934.1", "SO:0001217", "UniParc:UPI00000C1BDD", "UniRef:UniRef100_Q9PQR0", "UniRef:UniRef50_Q74L88", "UniRef:UniRef90_Q9PQR0" ], "nt": "ATGAAATCATTATTAGGAACAAAAGTTGGCATGACTCAAGTTTTTACAGAAACAGGTAAAGCTGTAGCTGCCACAGTTATCTATGTTGAACCAAATAAGGTTTTAGCTGTTAAAACTAATGAAAAAGACGGCTACAGTGCAATTCAAATTGGTTATGAAACAGTAAAAGAAAAAGCATTAAACAAACCATTATTAGGTCAATTCAAAAAAGCAAATTCAGATCCAAAACGTCACATTAAAGAATTTAGAGATGTTGTTGCTGAAGTTGGTGCTGAATTAACAGTTAGTGAATTTGAACCAGGACAATTAGTTAATGCACAAGCATATACTAAAGGACACGGATTTACAGGTTCAATTAAACGTCATAATTTTAGTATGGGTCCAATGGGCCATGGTGCTGGTTATCCACATCGTTATGTTGGTTCTATTGCTAAAGGACGTGGGGGTAGTCAAGCTCAACGTGTTTTCAAAGGAACTAAATTACCTGGTCATTATGGACATGAATTAGTAACAACTAAAAACTTACTAGTATTAGATGTTAAAGCAAATGAAAATTTAATCCTAATTAAAGGTGCTATTCCAGGACCAAAAGGTTCAATTGTTTTATTAAAATCAGCTAAAAAAGTTGGCCATATCGTAAGCGATCCACAAGTTGTTAACTACTTAGCTAATAAAGCATCATCAAGTGAAGCAAACGAATAA", "aa": "MKSLLGTKVGMTQVFTETGKAVAATVIYVEPNKVLAVKTNEKDGYSAIQIGYETVKEKALNKPLLGQFKKANSDPKRHIKEFRDVVAEVGAELTVSEFEPGQLVNAQAYTKGHGFTGSIKRHNFSMGPMGHGAGYPHRYVGSIAKGRGGSQAQRVFKGTKLPGHYGHELVTTKNLLVLDVKANENLILIKGAIPGPKGSIVLLKSAKKVGHIVSDPQVVNYLANKASSSEANE", "aa_hexdigest": "366a1842e7e2b4fd9559aab16212d569", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C1BDD", "ncbi_nrp_id": "WP_006688934.1", "uniref100_id": "UniRef100_Q9PQR0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BDD", "RefSeq:WP_006688934.1", "UniRef:UniRef100_Q9PQR0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQR0", "uniref90_id": "UniRef90_Q9PQR0" }, "psc": { "uniref90_id": "UniRef90_Q9PQR0", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q74L88", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q74L88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q74L88" ], "product": "Large ribosomal subunit protein uL3" }, "genes": [ "rplC" ], "id": "GPKLONFDEH_487", "locus": "GCA000006625_00454" }, { "type": "cds", "sequence": "contig_1", "start": 276857, "stop": 277051, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAATTAAATTATTATCTATTGATGGTAACTTCGCAAAAGAACTTGAAGTAACCTCTGATCTTTTTGTTGAAGTACCTCACAAACAAGCAATGTTTGACTCAGTGCTTGCTGAAAATGCAGCTGAACGTCAAGGTACACATTCAACCCTAACAAAGGGTGAAGTTCGTGGTGGTGGTAAAAAACCATGA", "aa": "MAKIKLLSIDGNFAKELEVTSDLFVEVPHKQAMFDSVLAENAAERQGTHSTLTKGEVRGGGKKP", "aa_hexdigest": "4721814174a0806d1bd3379485ec955d", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6926.840800000001, "isoelectric_point": 6.71930103302002 }, "id": "GPKLONFDEH_488", "locus": "GCA000006625_00455" }, { "type": "cds", "sequence": "contig_1", "start": 277118, "stop": 277399, "strand": "+", "frame": 2, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904", "SO:0001217", "UniRef:UniRef50_Q9PQQ9", "UniRef:UniRef90_Q9PQQ9" ], "nt": "GTGGTATTTGGTCCAAAACCAAATCGTAACTACAATTTAAAAGTTAATGCAAAAGTAAGACTATTAGCTTTCAAATCAGCATTAACTATTAAATTGAATGAAGGAAAAATGCTAGGTTTAGTAGCAAATAGCGATTTAGAAACACCTTCAACTAAAAAAATGGTAAATTTCATTAATAATGCTAACTTAGAAAATCAAAAAGTTTTATTAGTAATAGTCGACAATTTTAGTAATATTAAAAAATCAACAAACAATTTACAAAAAGTTACAACAAAATTATGA", "aa": "MVFGPKPNRNYNLKVNAKVRLLAFKSALTIKLNEGKMLGLVANSDLETPSTKKMVNFINNANLENQKVLLVIVDNFSNIKKSTNNLQKVTTKL", "aa_hexdigest": "5716e72a7e9a10ae23dcb952041a31a2", "start_type": "GTG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "rplD" ], "truncated": "both", "pseudogene": { "start": 276857, "stop": 277492, "observations": { "mutation": [ 277049, 277103, 277397 ], "stop": [ 277049, 277103, 277397 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQQ9", "query_cov": 1.0, "subject_cov": 0.44075829383886256, "identity": 0.9890000000000001, "score": 174.0, "evalue": 1.12e-53, "gene_start": 1, "gene_end": 93, "reference_start": 88, "reference_end": 180, "reference_sequence": "MAKIKLLSIDGNFAKELEVTSDLFVEVPHKQAMFDSVLAENAAERQGTHSTLTKGEVRGGGKKPWRQKHTGKARTGSTRNPHWTGGGVVFGPKPNRNYNLKVNAKVRLLAFKSALTIKLNEGKMLGLVANSDLETPSTKKMVNFINNANLENQKVLLVIVDNFSNIKKSTNNLQKVTTKLWYQVSVRDLMHANVVVVAEEAFTNYARKVSK" }, "paralog": false, "identity": 0.985781990521327, "score": 402.0, "evalue": 6.57e-146, "description": "Internal stop codon at 277049, 277103, 277397. Nonsense mutation around 277049, 277103, 277397.", "psc": { "uniref90_id": "UniRef90_Q9PQQ9", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_Q9PQQ9", "cog_id": "COG0088", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQQ9", "UniRef:UniRef50_Q9PQQ9", "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ9" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "GPKLONFDEH_489", "locus": "GCA000006625_00456" }, { "type": "cds", "sequence": "contig_1", "start": 277495, "stop": 277812, "strand": "+", "frame": 1, "gene": "rplW", "product": "50S ribosomal protein L23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_006689129.1", "SO:0001217", "UniParc:UPI00000C1BDF", "UniRef:UniRef100_Q9PQQ8", "UniRef:UniRef50_Q9PQQ8", "UniRef:UniRef90_Q9PQQ8" ], "nt": "ATGGAATTAACAAGAGTTATTTTACATCCATATACGACAGAAAAAACTTATTCTATTCGTAATAAAAGCGAGCATGAAACATTAACTTTTATTGTTGATAAAAATGCTAATAAATACCAAATTCGTGAAGCATTTATTGCGATTTTTGGTTTAAAACCACTTAAAATTCGTACTACAAATCGTCGTCCTGCAAAAATTCGTACTTCAACAGCTAGACCAGGATATACAAAAGCAAAAAAAATTGCTTATGTAGTAATGCCAGTAGGTGTTAAAGTAGCTGTAAGTAAAGAAGAAGTTGAAGCAGCAAACGCAAAGTAG", "aa": "MELTRVILHPYTTEKTYSIRNKSEHETLTFIVDKNANKYQIREAFIAIFGLKPLKIRTTNRRPAKIRTSTARPGYTKAKKIAYVVMPVGVKVAVSKEEVEAANAK", "aa_hexdigest": "fe0da67b84e0da48927f183a1a661b81", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI00000C1BDF", "ncbi_nrp_id": "WP_006689129.1", "uniref100_id": "UniRef100_Q9PQQ8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BDF", "RefSeq:WP_006689129.1", "UniRef:UniRef100_Q9PQQ8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQQ8", "uniref90_id": "UniRef90_Q9PQQ8" }, "psc": { "uniref90_id": "UniRef90_Q9PQQ8", "gene": "rplW", "product": "50S ribosomal protein L23", "uniref50_id": "UniRef50_Q9PQQ8", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:1990904", "GO:0005840", "GO:0019843", "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ8" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "GPKLONFDEH_490", "locus": "GCA000006625_00457" }, { "type": "cds", "sequence": "contig_1", "start": 277866, "stop": 278627, "strand": "+", "frame": 3, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "KEGG:K02886", "SO:0001217", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_A0A084F1R4" ], "nt": "ATGGCAGTTAAACGTATTAAAAACCATAGTAGTGGTAAAAGACAAACTGTTGTTGTTGATTACAAATCGATTTTAACAACAAGTAAACCAGAAAAATCATTGCTTGTTACTTTACCTAAAAAAGCAGGTAGAAACAATCAAGGGAAAATTACAATTAGACACCATGGTGGTGGTCATAAACGTAAATATCGTATTATTGACTTTAAAAGAAATAAAGATAATATTTATGGAACTATTAAATCAATTGAATACGATCCAAACAGAACATCATTTATTTCATTAGTTGTTTACGCTGATGGAGAAAAACGTTATATTATTGCTCCTAAAGGAATTAAAGTGGGCGATAAAATTATTTCAGGAAATGAAAACATTGATATTTTATTAGGTAATTCATTACCACTTGAATTTATTCCAGAAGGTACATTAGTTCACAATATTGAACTATCACCAAATGCTGGTGGTCAAATTACAAGAAGTGCTGGTGCTAGTGCACAAATTCTTGGTTTTGATGAAACTAAAAAATATATTTTAGTTAAATTAAACTCAGGCGAAGTTCGTAAATTTAGAAAAGAATGTCGTGCTACAATTGGAACTGTTTCAAATGATGAACACATTCTTGAAAATTTAGGTAAAGCTGGTAAATCACGTCATTTAGGGGTTCGTCCAACAGTTCGTGGTTCTGCAATGAACCCTAATGATCACCCACATGGGGGTGGTGAAGGTCGTTCACCAGTTGGTATGGATGCACCTCGTACACCTTGA", "aa": "MAVKRIKNHSSGKRQTVVVDYKSILTTSKPEKSLLVTLPKKAGRNNQGKITIRHHGGGHKRKYRIIDFKRNKDNIYGTIKSIEYDPNRTSFISLVVYADGEKRYIIAPKGIKVGDKIISGNENIDILLGNSLPLEFIPEGTLVHNIELSPNAGGQITRSAGASAQILGFDETKKYILVKLNSGEVRKFRKECRATIGTVSNDEHILENLGKAGKSRHLGVRPTVRGSAMNPNDHPHGGGEGRSPVGMDAPRTP", "aa_hexdigest": "1ded039aebb1719abd9f5df0a7913506", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A084F1R4", "query_cov": 1.0, "subject_cov": 0.9068100358422939, "identity": 0.925, "score": 476.0, "evalue": 6.95e-169, "valid": true, "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "GPKLONFDEH_491", "locus": "GCA000006625_00458" }, { "type": "cds", "sequence": "contig_1", "start": 278726, "stop": 278827, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAGAAGTTTAAAAAAAGGTGCTTACGCTGATCCTAGTTTATTAAAAAAAGTTGAAGCAGCTAATGCATCAGTTTCTAAAAAACCAATCAAAACATGA", "aa": "MSRSLKKGAYADPSLLKKVEAANASVSKKPIKT", "aa_hexdigest": "94b2bc5537068311c6202d944caf68bd", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3518.1343000000006, "isoelectric_point": 10.218255043029785 }, "id": "GPKLONFDEH_492", "locus": "GCA000006625_00459" }, { "type": "cds", "sequence": "contig_1", "start": 279014, "stop": 279775, "strand": "+", "frame": 2, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "COG:COG4933", "COG:K", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02890", "SO:0001217", "UniRef:UniRef50_Q9PQQ5", "UniRef:UniRef90_Q9PQQ5" ], "nt": "ATGACAAATAAAGTTATTCAAAGAAATATTCATATTTCGCACCGTAAAGCTTCTCTTGTAATTGATTTAGTTCGAAACAAGCCAGTACATGAAGCAATTCGAATTTTAAGTAATACACCAAAGAAGTTTGCTCCAATCGTTTTGAAATTACTTAACTCAGCAATTTCAAACGTTCAACATAACTCTAAAGATATGGATCCAAGTAAACTATATATTTACAAAATTGTAGCTAATCAAGGTCCAACAATGAAAAGAACACTACCACGTGCTAAGGGTTCTGCTGATCAATTATTTAAACGTACAACACATTTAGAAATTGTTTTATCTGATGATGTTAATGAACGTGAAAAAGAATTAGCAGCTATTAAAGCTAAAAAATCAAAAAAACCATTAGCAGTTGAACCGATTGCTAAAGTTGAAACTAAAAAAGTTGCTAAACCAAGCAAAGTAGAAATAAAACCAGTTGAAAAAGACGAAAACGTTGATCCAGAACTTTTAAAACGTGAACAACAAGTTTTAAAAGTTGTTGAAAAAACTGCTTCACAAAAAGAAGAAGAAACTACTGAAACAATTATGATTTCAACATCACCTAAGAATGCACAAGTTTTATTTGATGATTTAGAAAAAAATGTAATCTTTTATAAAACAACACCTATTAATAAAGTTTTACGTGTACTAGTTTATGTAACTAGCCCAACTAAAAAAGTTGTTGGTGAATTTGATTTAGAATCAGTTGAAATTGGTGCAATTTCATCAATTTGA", "aa": "MTNKVIQRNIHISHRKASLVIDLVRNKPVHEAIRILSNTPKKFAPIVLKLLNSAISNVQHNSKDMDPSKLYIYKIVANQGPTMKRTLPRAKGSADQLFKRTTHLEIVLSDDVNEREKELAAIKAKKSKKPLAVEPIAKVETKKVAKPSKVEIKPVEKDENVDPELLKREQQVLKVVEKTASQKEEETTETIMISTSPKNAQVLFDDLEKNVIFYKTTPINKVLRVLVYVTSPTKKVVGEFDLESVEIGAISSI", "aa_hexdigest": "931fdab34d4124e80584e8056c2e7041", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PQQ5", "query_cov": 1.0039525691699605, "subject_cov": 0.8141025641025641, "identity": 0.98, "score": 453.0, "evalue": 1.57e-159, "valid": true, "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_Q9PQQ5", "cog_id": "COG4933", "cog_category": "K", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ5" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "GPKLONFDEH_493", "locus": "GCA000006625_00460" }, { "type": "cds", "sequence": "contig_1", "start": 280323, "stop": 280580, "strand": "+", "frame": 3, "gene": null, "product": "30S ribosomal protein S3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6MUE3" ], "nt": "ATGGCACGTGAAATTGCCGACGCAATTGAAAATAGAGTTTCATTTAGAATTGCTCAAAAAATGGTAATTAAAAAAGTTCTAAAAGCTGGTGCTAGAGGTATTAAAACACATGTTTCAGGTCGTTTAGGTGGCGTGGAAATGGCACGTGAAGAAGGATATACACAAGGTGTAATGACCTTACATACATTACGTGCAGATATTGATTATTCAATGCAAGAAGCACATACAACTTATGGAATTATTGGTGTTAAAGTTTGA", "aa": "MAREIADAIENRVSFRIAQKMVIKKVLKAGARGIKTHVSGRLGGVEMAREEGYTQGVMTLHTLRADIDYSMQEAHTTYGIIGVKV", "aa_hexdigest": "673c83f50747cd1fdec06c08f25e803d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031CD8184", "query_cov": 0.9529411764705882, "subject_cov": 0.84375, "identity": 0.63, "score": 102.0, "evalue": 1.42e-26, "valid": false, "gene": "rpsC", "product": "30S ribosomal protein S3", "uniref50_id": "UniRef50_A0A7V6MUE3", "kegg_orthology_id": "K02982", "go_ids": [ "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7V6MUE3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6MUE3" ], "product": "30S ribosomal protein S3" }, "genes": [], "id": "GPKLONFDEH_494", "locus": "GCA000006625_00461" }, { "type": "cds", "sequence": "contig_1", "start": 280757, "stop": 280879, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00252.24" ], "nt": "ATGTTACAACCAAAAAGAACAAAATTTCGTAAGCCTCATAAAGTAAGCTACGAAGGTAAAGCTAAAGGAAATAAACAAGTTGATTTTGGTGAATTTGGATTAATGGCACTAGAAGGTGCATGA", "aa": "MLQPKRTKFRKPHKVSYEGKAKGNKQVDFGEFGLMALEGA", "aa_hexdigest": "29b04c372ff18cbedce23b5c04d62a02", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00252.24", "name": "Ribosomal_L16", "length": 37, "aa_cov": 0.9, "hmm_cov": 0.972972972972973, "evalue": 3.15353034097233e-08, "score": 29.23223876953125, "start": 4, "stop": 39 } ], "seq_stats": { "molecular_weight": 4523.2456999999995, "isoelectric_point": 9.9959680557251 }, "id": "GPKLONFDEH_495", "locus": "GCA000006625_00462" }, { "type": "cds", "sequence": "contig_1", "start": 281063, "stop": 281173, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAATTGCTAATGTTTCAGAAGAATTAATGTGTGAAGCTTTACGTGCTGCTGGAAATAAGTTACCAATTAAAGTAAAAATTGTTAAGAAGGGAGAAGCTAACTAA", "aa": "MFEIANVSEELMCEALRAAGNKLPIKVKIVKKGEAN", "aa_hexdigest": "13c37d9a40916fbdc6e9b3b7a962e545", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3945.672200000001, "isoelectric_point": 7.90119686126709 }, "id": "GPKLONFDEH_496", "locus": "GCA000006625_00463" }, { "type": "cds", "sequence": "contig_1", "start": 281173, "stop": 281400, "strand": "+", "frame": 1, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_004026203.1", "SO:0001217", "UniParc:UPI0000133E9C", "UniRef:UniRef100_Q9PQQ2", "UniRef:UniRef50_Q9PQQ2", "UniRef:UniRef90_Q9PQQ2" ], "nt": "ATGAGTAGTATTGCACAAGATCTTCGTAAAAAAGATAGCTTAGAACTTGAAAAAATTGTTATTGAGCTTAAAGCCAAATTATTAGAACTTCGTTTTGCAGCTGCGAATGGGGAAGCAGAAAAACTTCATACAGCCAAAGAAATTCGTAAAACAATTGCACGTGCATTAACAATTTTAAATGAACGTGAATTAGCAGAAAAATTAAACAACAAGGAGGCTAATAAATAA", "aa": "MSSIAQDLRKKDSLELEKIVIELKAKLLELRFAAANGEAEKLHTAKEIRKTIARALTILNERELAEKLNNKEANK", "aa_hexdigest": "a9d5b64f250cc5d0daea5f598b680b0d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000133E9C", "ncbi_nrp_id": "WP_004026203.1", "uniref100_id": "UniRef100_Q9PQQ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133E9C", "RefSeq:WP_004026203.1", "UniRef:UniRef100_Q9PQQ2" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQQ2", "uniref90_id": "UniRef90_Q9PQQ2" }, "psc": { "uniref90_id": "UniRef90_Q9PQQ2", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_Q9PQQ2", "cog_id": "COG0255", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ2" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "GPKLONFDEH_497", "locus": "GCA000006625_00464" }, { "type": "cds", "sequence": "contig_1", "start": 281400, "stop": 281621, "strand": "+", "frame": 3, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "SO:0001217", "UniRef:UniRef50_Q9PQQ1", "UniRef:UniRef90_Q9PQQ1" ], "nt": "ATGGAAAGAAGCCGTCGTAAAGTTTTAGAAGGGTTAGTAGTTTCAGATAAAATGCAAAAAACTGTTGTTGTTAGTGTTGAAACTAAAAGCAAACACCCAATATATAGAAAACTTGTAATTAGTCATAAAAAATACCACGCACACAATGATAATGATGATGCCAAAGTTGGTGACCTTGTTGAAATCACTGAAACTCGTCCATTAAGTGCAACAAAAAATTGA", "aa": "MERSRRKVLEGLVVSDKMQKTVVVSVETKSKHPIYRKLVISHKKYHAHNDNDDAKVGDLVEITETRPLSATKN", "aa_hexdigest": "8960cdd2dfad6911a387dc2958864098", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_Q9PQQ1", "query_cov": 1.0, "subject_cov": 0.8690476190476191, "identity": 1.0, "score": 142.0, "evalue": 8.53e-43, "valid": true, "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_Q9PQQ1", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQQ1" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "GPKLONFDEH_498", "locus": "GCA000006625_00465" }, { "type": "cds", "sequence": "contig_1", "start": 281674, "stop": 282042, "strand": "+", "frame": 1, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02874", "RefSeq:WP_004026223.1", "SO:0001217", "UniParc:UPI00000C1BE4", "UniRef:UniRef100_Q9PQQ0", "UniRef:UniRef50_A5FMZ0", "UniRef:UniRef90_Q9PQQ0" ], "nt": "ATGATTCAACATATGACAAGACTAAAAGTTGCAGATAACACAGGTGCTAAAGAAGTTGGAGTAATCAAAGTGCTTGGTGGCTCTAAAAAACGTTATGCATCAGTGGGCGATATTGTAGTTGTCTCAGTTAAAAAAGCAACACCTGCTGGTTTAATTGCTAAAGGACAAATGGCCAAAGCTGTAATTGTTAGAACAAAAAAATCAATTCGTCGTGAGTCTGGTTTATTAATTAGATTTGACGAAAACGCATGTGTACTTATTAAAGAGGATAAGACACCAAGAGGATCACGTATTTTTGGTCCTGTAGCAAGAGAAATTCGTGATCGTGGATACACAAAAATTGCTTCTCTTGCTCCTGAAGTATTATAG", "aa": "MIQHMTRLKVADNTGAKEVGVIKVLGGSKKRYASVGDIVVVSVKKATPAGLIAKGQMAKAVIVRTKKSIRRESGLLIRFDENACVLIKEDKTPRGSRIFGPVAREIRDRGYTKIASLAPEVL", "aa_hexdigest": "2e62b6cc554c5edb854b3d4f2179b4f3", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BE4", "ncbi_nrp_id": "WP_004026223.1", "uniref100_id": "UniRef100_Q9PQQ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BE4", "RefSeq:WP_004026223.1", "UniRef:UniRef100_Q9PQQ0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQQ0", "uniref90_id": "UniRef90_Q9PQQ0" }, "psc": { "uniref90_id": "UniRef90_Q9PQQ0", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_A5FMZ0", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_A5FMZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5FMZ0" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "GPKLONFDEH_499", "locus": "GCA000006625_00466" }, { "type": "cds", "sequence": "contig_1", "start": 282057, "stop": 282389, "strand": "+", "frame": 3, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_006688875.1", "SO:0001217", "UniParc:UPI00000C1BE5", "UniRef:UniRef100_Q9PQP9", "UniRef:UniRef50_O52343", "UniRef:UniRef90_Q9PQP9" ], "nt": "ATGAACAGAATTAAAAAAGGCGATACAGTTGTTGTTATTTCAGGTAAAAACAAAAATAAATCTGGCGTAGTTATTCAAGTTAACCCTAAAGAACAAACAGCACTTGTTGAAGGCGTTAATAAAATTAAACGTCATCAAAAGAAAGATCAAACGCATGAACAATCTGGAATTATTGAAAAAGAAGCTCCAATTCGTTTATGTAAATTAGCATTAGTTGATCCTAAAGGAAAAGATAAAGGTAAAGCAACTAAAGTTAAATATTTACTTAAAGATAACAAAAAAGTTCGTGTAGCACGTAAAAGTGGTTCAGAACTTGATGTAAATAAAAAATAA", "aa": "MNRIKKGDTVVVISGKNKNKSGVVIQVNPKEQTALVEGVNKIKRHQKKDQTHEQSGIIEKEAPIRLCKLALVDPKGKDKGKATKVKYLLKDNKKVRVARKSGSELDVNKK", "aa_hexdigest": "896ddee55bf86af351d6a914358a9e59", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1BE5", "ncbi_nrp_id": "WP_006688875.1", "uniref100_id": "UniRef100_Q9PQP9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BE5", "RefSeq:WP_006688875.1", "UniRef:UniRef100_Q9PQP9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQP9", "uniref90_id": "UniRef90_Q9PQP9" }, "psc": { "uniref90_id": "UniRef90_Q9PQP9", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_O52343", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_O52343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52343" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "GPKLONFDEH_500", "locus": "GCA000006625_00467" }, { "type": "cds", "sequence": "contig_1", "start": 282406, "stop": 282696, "strand": "+", "frame": 1, "gene": "rplE", "product": "50S ribosomal protein L5", "db_xrefs": [ "COG:COG0094", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02931", "SO:0001217", "UniRef:UniRef50_Q9PQP8", "UniRef:UniRef90_Q9PQP8" ], "nt": "ATGGCATTCTTAAAAGATTTATATAAAAATAAAGTTGCTAAAGATTTGCAAAAAGAATTTGCTTATTCTTCTGTAATGCAAATTCCTAAAATTGAAAAAGTAGTTATTAACGCAGGAATTGGAAATGCTGTTGCTGATAAAAAACATTTAGAAGCAGCTATTTCAGAATTAACTTTGATTACAGGACAAAGACCTGTTGAAACAAAAGCTAAAAAATCAATTGCAACATTTAAATTACGTGCTGGTCAAAGTATTGGTGCTAAAGTTACATTACGTGGCGATCGTATGTGA", "aa": "MAFLKDLYKNKVAKDLQKEFAYSSVMQIPKIEKVVINAGIGNAVADKKHLEAAISELTLITGQRPVETKAKKSIATFKLRAGQSIGAKVTLRGDRM", "aa_hexdigest": "ce18672de5e0948748c2c5e551fad492", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001BFFEF50", "query_cov": 1.0, "subject_cov": 0.8648648648648649, "identity": 0.563, "score": 111.0, "evalue": 7.54e-30, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A2X1PYD2", "kegg_orthology_id": "K02931" }, "genes": [ "rplE" ], "truncated": "3-prime", "pseudogene": { "start": 282406, "stop": 282960, "observations": { "mutation": [ 282694 ], "stop": [ 282694 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP8", "query_cov": 1.0, "subject_cov": 0.5217391304347826, "identity": 1.0, "score": 181.0, "evalue": 1.44e-56, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "MAFLKDLYKNKVAKDLQKEFAYSSVMQIPKIEKVVINAGIGNAVADKKHLEAAISELTLITGQRPVETKAKKSIATFKLRAGQSIGAKVTLRGDRMWAFIETLFNIALPRVRDFKGISNNSFDNQGNYTLGIKEQIIFPQVVYDDVKSVRGFDVTFVTTAKTAQEAKALLVGLGAPFQKVRGDK" }, "paralog": false, "identity": 0.9945652173913043, "score": 352.0, "evalue": 1.74e-126, "description": "Internal stop codon at 282694. Nonsense mutation around 282694.", "psc": { "uniref90_id": "UniRef90_Q9PQP8", "gene": "rplE", "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_Q9PQP8", "cog_id": "COG0094", "cog_category": "J", "kegg_orthology_id": "K02931", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP8", "UniRef:UniRef50_Q9PQP8", "COG:COG0094", "COG:J", "KEGG:K02931", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP8" ], "product": "Large ribosomal subunit protein uL5" } }, "id": "GPKLONFDEH_501", "locus": "GCA000006625_00468" }, { "type": "cds", "sequence": "contig_1", "start": 282850, "stop": 282960, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTGGTTTTGATGTAACATTTGTTACAACTGCAAAAACAGCCCAAGAAGCTAAAGCATTACTAGTGGGACTAGGTGCTCCATTTCAAAAAGTAAGAGGAGACAAATAA", "aa": "MRGFDVTFVTTAKTAQEAKALLVGLGAPFQKVRGDK", "aa_hexdigest": "0933bb9427471704bac5bf4f1b649386", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3852.462800000001, "isoelectric_point": 9.991971015930176 }, "id": "GPKLONFDEH_502", "locus": "GCA000006625_00469" }, { "type": "cds", "sequence": "contig_1", "start": 282964, "stop": 283146, "strand": "+", "frame": 1, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "COG:COG0199", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_Q9PQP7", "UniRef:UniRef90_Q9PQP7" ], "nt": "ATGGCGAAAAAATCATTAATTGCTAAGCAAAAAAAACATCAAAAATTTGCAGTTCGTGAATACACTCGTTGCGTAAGATGTGGTCGTCCACATGCTGTTAATCGTAAATTTGGTGTATGTCGTCTTTGCTTTAGAGATTTAGCTTACGCTGGTGCAATTCCAGGAATTAAGAAAGCATCTTGA", "aa": "MAKKSLIAKQKKHQKFAVREYTRCVRCGRPHAVNRKFGVCRLCFRDLAYAGAIPGIKKAS", "aa_hexdigest": "1c4225f1605cc04d44260c747731166c", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_Q9PQP7", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 1.0, "score": 124.0, "evalue": 2.71e-36, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_Q9PQP7", "cog_id": "COG0199", "cog_category": "J", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [ "rpsZ" ], "id": "GPKLONFDEH_503", "locus": "GCA000006625_00470" }, { "type": "cds", "sequence": "contig_1", "start": 283160, "stop": 283555, "strand": "+", "frame": 2, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "SO:0001217", "UniRef:UniRef50_Q9PQP6", "UniRef:UniRef90_Q9PQP6" ], "nt": "ATGTATTTAGATCCAATTGCTGAATTAATTACAAAAATTAATAATGGTCGTAAGGCCCATAAAGCTGAAGTTAGTTTTGCAACTTCAAAGCTTAAAACAGCGATTTTAGAATTATTGGTTAAAGAAGGATATATCAAATCTTATGATATTCGTCCAACTGAAAACAATAAATCTGAAACAGTTGTTAAACTAAAATACAAAAACCAAACAACATCTTCTATCAATGGTTTTAAACAAATTTCAAAACCAGGATTAAGAATTTATTCAACACACTTAAATTTACCAAAAGTATTAAATGGTTTAGGAATTGCTATCATCACAACTTCAAAAGGTGTTATGTCAGATAAACAAGCAAGAAAGGAAAATGTAGGCGGGGAAGTAATCGCTTACGTTTGA", "aa": "MYLDPIAELITKINNGRKAHKAEVSFATSKLKTAILELLVKEGYIKSYDIRPTENNKSETVVKLKYKNQTTSSINGFKQISKPGLRIYSTHLNLPKVLNGLGIAIITTSKGVMSDKQARKENVGGEVIAYV", "aa_hexdigest": "1241dfa8efc1e17adcfe51df4943df54", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PQP6", "query_cov": 1.0, "subject_cov": 0.9924242424242424, "identity": 1.0, "score": 252.0, "evalue": 7.15e-85, "valid": true, "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q9PQP6", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP6" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "GPKLONFDEH_504", "locus": "GCA000006625_00471" }, { "type": "cds", "sequence": "contig_1", "start": 283567, "stop": 283818, "strand": "+", "frame": 1, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "SO:0001217", "UniRef:UniRef50_P47412", "UniRef:UniRef90_Q9PQP5" ], "nt": "ATGTCAAGAATTGGAAATCGTAAATTAACTATTCCAGCTAATGTAAATGTTAGTGTTGAATCTGGTAAAGTACATATTGTTAGTCAAACTGCAAAATTAAGTGTTGATTTTCCAGTAAATCTAATTAGTGTAGATGTTGTTGATAATACTGTTAAAGTTAGTCGTGCAAACGATGAAAAACAAACAAAAATGTTTCATGGAACAGTAAATGCAAATATTGCTAATGCTTTAGTTGGTGTAACTACTGGTTGA", "aa": "MSRIGNRKLTIPANVNVSVESGKVHIVSQTAKLSVDFPVNLISVDVVDNTVKVSRANDEKQTKMFHGTVNANIANALVGVTTG", "aa_hexdigest": "53fa92a1a5d0ab3be723ff27cc499450", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplF" ], "truncated": "3-prime", "pseudogene": { "start": 283567, "stop": 284115, "observations": { "mutation": [ 283816 ], "stop": [ 283816 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP5", "query_cov": 1.0, "subject_cov": 0.45604395604395603, "identity": 0.988, "score": 157.0, "evalue": 1.48e-47, "gene_start": 1, "gene_end": 83, "reference_start": 1, "reference_end": 83, "reference_sequence": "MSRIGNRKLTIPANVNVSVESGKVHIVSQTAKLSVDFPVNLISVDVVDNTIKVSRANDEKQTKMFHGTVNANIANALVGVTTGWKKELEVKGVGFRAKVEGSKLNLGLGFSHPLLIQIPTGLKIETPSATEISISGSDKAAVGAFAAVVRAYRKPEPYKGKGVMYKGERIVRKAGKTADKKK" }, "paralog": false, "identity": 0.9725274725274725, "score": 345.0, "evalue": 3.84e-124, "description": "Internal stop codon at 283816. Nonsense mutation around 283816.", "psc": { "uniref90_id": "UniRef90_Q9PQP5", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_P47412", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP5", "UniRef:UniRef50_P47412", "COG:COG0097", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P47412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47412" ], "product": "Large ribosomal subunit protein uL6" } }, "id": "GPKLONFDEH_505", "locus": "GCA000006625_00472" }, { "type": "cds", "sequence": "contig_1", "start": 284134, "stop": 284268, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAATTAATTTTAGTCGTGCTAAACAACGTGCACTTCGTGCTAAACGTCTTCATGTTAAAATTCGTAACCTTCAATTAGCTGCAAACAAACCTGTTTTAGTTATTACAAAAACAAATGCACACATTTGA", "aa": "MKRINFSRAKQRALRAKRLHVKIRNLQLAANKPVLVITKTNAHI", "aa_hexdigest": "bcc7f38189c474b2819c8b763ad955f1", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5118.1596, "isoelectric_point": 11.999967765808105 }, "id": "GPKLONFDEH_506", "locus": "GCA000006625_00473" }, { "type": "cds", "sequence": "contig_1", "start": 284517, "stop": 285128, "strand": "+", "frame": 3, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "COG:COG0098", "COG:J", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02988", "RefSeq:WP_006688963.1", "SO:0001217", "UniParc:UPI0000135080", "UniRef:UniRef100_Q9PQP3", "UniRef:UniRef50_B5ZB57", "UniRef:UniRef90_B5ZB57" ], "nt": "ATGGAAAACAATGTTAAAAAAGAAACTATTGTAGATAGTGAAAAAGTTGAAAAGCAACAACTAGTAACTGCACCAGTAGTTAATAAAAAAGAAAATACAGAACCAAAAGCAAAAACATTTAAACGTGAAACAACAACATCAAATTTTGAAGAACGTGTAGTGAAAATTAAACGAATTTCAAAAACAACAAAAGGTGGACGTATGATGCGTTTTAGTGCTTTAGTTGTTATTGGTGATAAAAATGGAACTGTTGGTTTTGGTATGGGTAAATCAATTGAAGTTCCAGATGCAATCAAAAAAGCGATTAAAAACGCAAACAATAATTTAATTAAAGTTAAGCAAACAAAAAAAGGTTCAATTTACCATGATGTAAATGGTCGTCATGGAGCTGCTAAAGTAATGTTACTACCTGCTCCTGAAGGTACTGGAATTATTGCTGGTGGACCTGTACGTGCTGTGGTTGAATTAGCTGGATTTACTGATATTTATACAAAATCACGTGGTGCTAATGCGCCAATGAATGTTATTCGTGCCACAATCAATGGGTTATTACAACAATTAACACCAAAAGAAATTGCACGTTTACGTGACAAAAGTTTAAGAGAAATATAG", "aa": "MENNVKKETIVDSEKVEKQQLVTAPVVNKKENTEPKAKTFKRETTTSNFEERVVKIKRISKTTKGGRMMRFSALVVIGDKNGTVGFGMGKSIEVPDAIKKAIKNANNNLIKVKQTKKGSIYHDVNGRHGAAKVMLLPAPEGTGIIAGGPVRAVVELAGFTDIYTKSRGANAPMNVIRATINGLLQQLTPKEIARLRDKSLREI", "aa_hexdigest": "03011c2430be4e3a61b66f220ae969b9", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000135080", "ncbi_nrp_id": "WP_006688963.1", "uniref100_id": "UniRef100_Q9PQP3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135080", "RefSeq:WP_006688963.1", "UniRef:UniRef100_Q9PQP3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQP3", "uniref90_id": "UniRef90_B5ZB57" }, "psc": { "uniref90_id": "UniRef90_B5ZB57", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_B5ZB57", "cog_id": "COG0098", "cog_category": "J", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZB57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZB57" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "GPKLONFDEH_507", "locus": "GCA000006625_00474" }, { "type": "cds", "sequence": "contig_1", "start": 285145, "stop": 285591, "strand": "+", "frame": 1, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02876", "RefSeq:WP_006688932.1", "SO:0001217", "UniParc:UPI00000C1BE9", "UniRef:UniRef100_Q9PQP2", "UniRef:UniRef50_Q9PQP2", "UniRef:UniRef90_Q9PQP2" ], "nt": "ATGCAATTACATAATTTAGAATATAAAAAAGGTTCAAGAAACCACAAAGAAAAACGTGTTGGTCGTGGTCATGGTTCTGGTTTAGGAAAAACTTCTGGGCGTGGACAAGATGGACAAAAAGCACGTAAATCAGGAATGGTACGTTTAGCTTTTGAAGGAGGACAAACACCACTTTATCGTCGTGTGCCAAAAGTTGGTTTTAACAACGATCGTTTTGCAAATAAATATAACGTTGTAACTTTAATTTCATTAGTAAAATATGAAACAAAAGAATTAACAGTTGAATTTATGTATGCAAATAAAATTGCTAAAAATGAAGATTTACCAATTAAGGTTATAGGTAATGCAGTTTTACCAAGTGGCACAGTAGTAAGTGCTCACAAATTTAGTAAAGGCGCATTGGAATCAATTAGTAATTCAAAAGCAAAAGCACAAATTTTAGAGTAA", "aa": "MQLHNLEYKKGSRNHKEKRVGRGHGSGLGKTSGRGQDGQKARKSGMVRLAFEGGQTPLYRRVPKVGFNNDRFANKYNVVTLISLVKYETKELTVEFMYANKIAKNEDLPIKVIGNAVLPSGTVVSAHKFSKGALESISNSKAKAQILE", "aa_hexdigest": "098c8bbdc49d02b044f81e6b4bce33b3", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BE9", "ncbi_nrp_id": "WP_006688932.1", "uniref100_id": "UniRef100_Q9PQP2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BE9", "RefSeq:WP_006688932.1", "UniRef:UniRef100_Q9PQP2" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQP2", "uniref90_id": "UniRef90_Q9PQP2" }, "psc": { "uniref90_id": "UniRef90_Q9PQP2", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_Q9PQP2", "cog_id": "COG0200", "cog_category": "J", "kegg_orthology_id": "K02876", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP2" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "GPKLONFDEH_508", "locus": "GCA000006625_00475" }, { "type": "cds", "sequence": "contig_1", "start": 285644, "stop": 286606, "strand": "+", "frame": 2, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952", "SO:0001217", "UniRef:UniRef50_Q9PQP1", "UniRef:UniRef90_Q9PQP1" ], "nt": "ATGACTAACAAACAGAAGAAAAAAAATGCTTTTAGACAGTTGTTAATGATCTTTAAAAATAAAAAAGTTTTAGTTGCATTAATTGTAACTTTAAGTATATTAATCTTGTTTAGAATTGGATCTGTTATCCCAATGCCATACATTAAGTTAAATGGTAATTTTGGCAATCAAGGTTCATTCTTTTCAATTATTAACTTATTAGGCGGGGGAGGGCTATCACAATTTTCACTCTTCGCAATTGGGATAGGACCTTATATTACCGCTCAAATTATTATGCAACTTTTATCAAGTGAGCTTGTGCCACCACTCGCCAAATTATCCAAATCAGGTGAGCGTGGTCGTAAAAAAATTGAAGTGATTACAAGAATCATTACCTTGCCTCTTGCAGTGATGCAAGCTGTCATTATTATTAATTTAATGACAAGAGCAAATGGATTTATTTCAATTGTTTCAAATGCACCATTTGCTATTGGTTCACCATTATTTTATGTAACATACATTTTTTTAATGGTTGGTGGCACTTATATTTCGTTATTTTTAGCTGATCTAATTTCGAAAAAAGGTGTTGGTAATGGAATTACATTATTAATTCTTACAGGAATTGTTGCTTCATTATTTAATCACTTTATTGCAATTTTTTCAAATTTAGGTAGTTTAACAAGCAGTAAAGTTTCACAAATTATCGGTTTCATTTTATACATTTTATTCTATATTATGATCTTAATTGGTGTTGTTTTTGTTAATAATTCAACAAGAAAAATTCCAGTTCAACAAACCGGTCAAGCTCTAATTTTAGATCATGAGAAACTACCATTTCTTCCTATTAAAATTATGACAGCAGGAGTAATGCCAGTGATCTTTGCATCATCTGTTTTAGCCATTCCTGCACAAGTTGCTGAATTTTTAGATAAACAAAGCATGGGTTATTATGTGATTCATAATTACTTCATTGTTGATTCTTGA", "aa": "MTNKQKKKNAFRQLLMIFKNKKVLVALIVTLSILILFRIGSVIPMPYIKLNGNFGNQGSFFSIINLLGGGGLSQFSLFAIGIGPYITAQIIMQLLSSELVPPLAKLSKSGERGRKKIEVITRIITLPLAVMQAVIIINLMTRANGFISIVSNAPFAIGSPLFYVTYIFLMVGGTYISLFLADLISKKGVGNGITLLILTGIVASLFNHFIAIFSNLGSLTSSKVSQIIGFILYILFYIMILIGVVFVNNSTRKIPVQQTGQALILDHEKLPFLPIKIMTAGVMPVIFASSVLAIPAQVAEFLDKQSMGYYVIHNYFIVDS", "aa_hexdigest": "f1c20715df3394c417a98113be9a52b1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secY" ], "truncated": "3-prime", "pseudogene": { "start": 285644, "stop": 286909, "observations": { "mutation": [ 286604, 286784 ], "stop": [ 286604, 286784 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP1", "query_cov": 1.0, "subject_cov": 0.6794055201698513, "identity": 0.9940000000000001, "score": 578.0, "evalue": 3.27e-205, "gene_start": 1, "gene_end": 320, "reference_start": 1, "reference_end": 320, "reference_sequence": "MTNKQKKKNAFRQLLMIFKNKKVLVALIVTLSILILFRIGSVIPMPYIKLNGNFGNQGSFFSIINLLGGGGLSQFSLFAIGIGPYITAQIIMQLLSSELVPPLAKLSKSGERGRKKIEVITRIITLPLAVMQAVIIINLMTRANGFISIVPNAPFAIGSPLFYVTYIFLMVGGTYISLFLADLISKKGVGNGITLLILTGIVASLFNHFIAIFSNLGSLTSSKVSQIIGFILYILFYIMILIGVVFVNNSTRKIPIQQTGQALILDHEKLPFLPIKIMTAGVMPVIFASSVLAIPAQVAEFLDKQSMGYYVIHNYFIVDSWTGLAIYVVLILLFTFFFSYVQLNPPKMAEDIKKAGRFIPGVQVGMDTEKHITKVIYRVNWIGAPILAFLACLPHLVALVAKTINHGIPVIQPSTIFGGTSIIIMVTATLELWNAIKSTSTSTSYAYQRKELETAITISVESDKSSKSQIW" }, "paralog": false, "identity": 0.9904988123515439, "score": 797.0, "evalue": 3.99e-294, "description": "Internal stop codon at 286604, 286784. Nonsense mutation around 286604, 286784.", "psc": { "uniref90_id": "UniRef90_Q9PQP1", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_Q9PQP1", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0015031", "GO:0016020", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP1", "UniRef:UniRef50_Q9PQP1", "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP1" ], "product": "Protein translocase subunit SecY" } }, "id": "GPKLONFDEH_509", "locus": "GCA000006625_00476" }, { "type": "cds", "sequence": "contig_1", "start": 286685, "stop": 286786, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGAAGATATTAAAAAAGCCGGTCGTTTTATTCCAGGTGTTCAAGTTGGAATGGATACTGAAAAACATATTACAAAAGTAATTTATCGCGTGAATTGA", "aa": "MAEDIKKAGRFIPGVQVGMDTEKHITKVIYRVN", "aa_hexdigest": "a88124ab4f4ab24139071ccfb96c6921", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.375900000001, "isoelectric_point": 9.397636985778806 }, "id": "GPKLONFDEH_510", "locus": "GCA000006625_00477" }, { "type": "cds", "sequence": "contig_1", "start": 286820, "stop": 286942, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTCATTTGGTTGCTTTAGTAGCTAAAACAATCAATCATGGAATTCCTGTAATTCAACCATCAACAATTTTTGGAGGAACTTCAATTATTATTATGGTTACAGCAACGCTTGAATTATGA", "aa": "MPHLVALVAKTINHGIPVIQPSTIFGGTSIIIMVTATLEL", "aa_hexdigest": "dd5d98eb190df900437e894e3032b5ed", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4198.0427, "isoelectric_point": 6.693041419982911 }, "id": "GPKLONFDEH_511", "locus": "GCA000006625_00478" }, { "type": "cds", "sequence": "contig_1", "start": 287068, "stop": 287442, "strand": "+", "frame": 1, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "SO:0001217", "UniRef:UniRef50_Q9PQP0", "UniRef:UniRef90_Q9PQP0" ], "nt": "ATGAAAATCCTTTTAATTGGCCCCCCAGGGAGTGGTAAGGGAAGTGTTAGTGAATTATTAACTAAAAATAATACATTAAAACATGTTTCCACAGGTAATTTATTTCGTGCAATTCTAAAAGAGGATAGCGAATTAGCACGTAAAATTAAAGAGATTAATGTTTCTGGTGGTAAATTAGTACCAGATGAAATTACTAATCAAGTTGCTAAATCAGCAATTGATGAATTAATTAAAAATGAACAATCATTTATTTTAGATGGTTATCCACGCACAATTAATCAAGCCTTAGCATTAGAACAATATTGTAATTTAGATTATATTTTTTATCTAGATATTGATCATCAAGAATTAATGAAACGTTTAACTGGGCGTTGA", "aa": "MKILLIGPPGSGKGSVSELLTKNNTLKHVSTGNLFRAILKEDSELARKIKEINVSGGKLVPDEITNQVAKSAIDELIKNEQSFILDGYPRTINQALALEQYCNLDYIFYLDIDHQELMKRLTGR", "aa_hexdigest": "f6548b976425739eb0ad0609b0cd0943", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "adk" ], "truncated": "3-prime", "pseudogene": { "start": 287068, "stop": 287709, "observations": { "mutation": [ 287440 ], "stop": [ 287440 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP0", "query_cov": 1.0, "subject_cov": 0.5821596244131455, "identity": 1.0, "score": 245.0, "evalue": 6.39e-81, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MKILLIGPPGSGKGSVSELLTKNNTLKHVSTGNLFRAILKEDSELARKIKEINVSGGKLVPDEITNQVAKSAIDELIKNEQSFILDGYPRTINQALALEQYCNLDYIFYLDIDHQELMKRLTGRWMCPKCAGIYNIHFKKPQVHGLCDNDQATLYQRADDHADAVSIRLDEYDKLTLPLIKHYETNPRFIKINANQPIEDVYKNINNYLKQNK" }, "paralog": false, "identity": 0.9953051643192489, "score": 429.0, "evalue": 5.11e-156, "description": "Internal stop codon at 287440. Nonsense mutation around 287440.", "psc": { "uniref90_id": "UniRef90_Q9PQP0", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q9PQP0", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP0", "EC:2.7.4.3", "UniRef:UniRef50_Q9PQP0", "COG:COG0563", "COG:F", "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP0" ], "product": "Adenylate kinase" } }, "id": "GPKLONFDEH_512", "locus": "GCA000006625_00479" }, { "type": "cds", "sequence": "contig_1", "start": 287443, "stop": 287709, "strand": "+", "frame": 1, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "SO:0001217", "UniRef:UniRef50_Q9PQP0", "UniRef:UniRef90_Q9PQP0" ], "nt": "ATGTGCCCAAAATGTGCAGGAATTTATAATATTCATTTTAAAAAACCACAAGTTCATGGTTTATGTGACAATGATCAAGCTACTCTTTATCAACGAGCTGATGATCATGCAGATGCTGTAAGTATTCGATTAGATGAATATGATAAATTAACCCTACCTTTGATTAAGCATTATGAAACTAATCCACGTTTTATTAAAATCAATGCTAATCAACCTATTGAAGATGTTTATAAAAACATTAACAATTATTTAAAACAAAATAAGTAA", "aa": "MCPKCAGIYNIHFKKPQVHGLCDNDQATLYQRADDHADAVSIRLDEYDKLTLPLIKHYETNPRFIKINANQPIEDVYKNINNYLKQNK", "aa_hexdigest": "a2c5862ecc8f11c219dfb21e321576fb", "start_type": "ATG", "rbs_motif": null, "genes": [ "adk" ], "truncated": "5-prime", "pseudogene": { "start": 287143, "stop": 287709, "observations": { "mutation": [ 287440 ], "stop": [ 287440 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQP0", "query_cov": 1.0, "subject_cov": 0.4131455399061033, "identity": 1.0, "score": 188.0, "evalue": 4.67e-59, "gene_start": 1, "gene_end": 88, "reference_start": 126, "reference_end": 213, "reference_sequence": "MKILLIGPPGSGKGSVSELLTKNNTLKHVSTGNLFRAILKEDSELARKIKEINVSGGKLVPDEITNQVAKSAIDELIKNEQSFILDGYPRTINQALALEQYCNLDYIFYLDIDHQELMKRLTGRWMCPKCAGIYNIHFKKPQVHGLCDNDQATLYQRADDHADAVSIRLDEYDKLTLPLIKHYETNPRFIKINANQPIEDVYKNINNYLKQNK" }, "paralog": false, "identity": 0.9946808510638298, "score": 381.0, "evalue": 1.38e-137, "description": "Internal stop codon at 287440. Nonsense mutation around 287440.", "psc": { "uniref90_id": "UniRef90_Q9PQP0", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q9PQP0", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQP0", "EC:2.7.4.3", "UniRef:UniRef50_Q9PQP0", "COG:COG0563", "COG:F", "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP0" ], "product": "Adenylate kinase" } }, "id": "GPKLONFDEH_513", "locus": "GCA000006625_00480" }, { "type": "cds", "sequence": "contig_1", "start": 287944, "stop": 288393, "strand": "+", "frame": 1, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0006508", "GO:0070006", "KEGG:K01265", "SO:0001217", "UniRef:UniRef50_Q9PQN9", "UniRef:UniRef90_Q9PQN9" ], "nt": "GTGAATGAGTGCGTTATTCATGGAGTCCCAACCGATTACGTCTTAAAAGATGGTGATAAGGTAACATTTGATGTTGGTGTTAAATACGATAATCATTATTGTGATGCGGCGTTTACCATTATTATTAACAACTCAAATGTTGAAGCACTAAAAATATCAGAGATTTGTAAAAAATCAATTGACGAAGCCGTTGCAATTATTAAACCAAAAGTAACAACACATGCAATTTCGAATGCAATTCAAAAGTTTATTGAAAAAAACGGTTATTTTGTTCTTCGTGATTTTGCCGGCCACGGTTGTGGTAATGAAATTCATGAAGATCCATTGATTCCTAATTATCGATCTTTATTGTATCGTAATGTTACATTAGAAGAGAATATGGTAATTTGTATTGAACCAATGATTCTAAGTGGATCAAATGCTTATTATATTGATCCAAACGATCAATGA", "aa": "MNECVIHGVPTDYVLKDGDKVTFDVGVKYDNHYCDAAFTIIINNSNVEALKISEICKKSIDEAVAIIKPKVTTHAISNAIQKFIEKNGYFVLRDFAGHGCGNEIHEDPLIPNYRSLLYRNVTLEENMVICIEPMILSGSNAYYIDPNDQ", "aa_hexdigest": "281e369c295f74129be37c20983a0a0d", "start_type": "GTG", "rbs_motif": null, "genes": [ "map" ], "truncated": "both", "pseudogene": { "start": 287734, "stop": 288480, "observations": { "mutation": [ 287791, 288391, 288430 ], "stop": [ 287791, 288391, 288430 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQN9", "query_cov": 1.0, "subject_cov": 0.5983935742971888, "identity": 0.966, "score": 304.0, "evalue": 3.06e-103, "gene_start": 1, "gene_end": 149, "reference_start": 71, "reference_end": 219, "reference_sequence": "MVIVKTEKDITAIKEAVRIWKIAREAIYEQVKAGVSLKELDLLAKEVIEANGGIAAFHNYLGFKGHICISVNECVIHGVPTDYILKDGDKVTFDVGVKYDNHYCDAAFTIIINNSNVEALKMSEICKKSIDEAVAIIKPKVTTHAISNVIQKFIEKNGYFILRDFAGHGCGNEIHEDPLIPNYRSLLYRNVTLEENMVICIEPMILSGSNAYYIDPNDQWSVKSKNHQMTCHWEHMILITKDGCEVLTA" }, "paralog": false, "identity": 0.9596774193548387, "score": 482.0, "evalue": 4.8e-176, "description": "Internal stop codon at 287791, 288391, 288430. Nonsense mutation around 287791, 288391, 288430.", "psc": { "uniref90_id": "UniRef90_Q9PQN9", "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_Q9PQN9", "cog_id": "COG0024", "cog_category": "J", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0006508", "GO:0070006" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQN9", "EC:3.4.11.18", "UniRef:UniRef50_Q9PQN9", "COG:COG0024", "COG:J", "KEGG:K01265", "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQN9" ], "product": "Methionine aminopeptidase" } }, "id": "GPKLONFDEH_514", "locus": "GCA000006625_00481" }, { "type": "cds", "sequence": "contig_1", "start": 288511, "stop": 288735, "strand": "+", "frame": 1, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022", "KEGG:K02518", "RefSeq:WP_006688844.1", "SO:0001217", "UniParc:UPI000012D274", "UniRef:UniRef100_Q9PQN8", "UniRef:UniRef50_Q6F1X1", "UniRef:UniRef90_Q9PQN8" ], "nt": "ATGGCAGATACAGAAAAATTAAAAATGCTTGGTAAAATTGTTGAAGTTTTACAAGGCGGAAATTTTAGAGTTCAATTAGAAAATGGCATCACGATTATGAGCCACGTATCAGGTAAAATGCGTGTTAATAAAATTAACATTTTACCAGGTGATACTGTTGATGTTGAGTTAAGTCCATACGACCTAACACGTGGAAGAATTACATATCGTCATCGTGATAACTAA", "aa": "MADTEKLKMLGKIVEVLQGGNFRVQLENGITIMSHVSGKMRVNKINILPGDTVDVELSPYDLTRGRITYRHRDN", "aa_hexdigest": "c8a6615a3951a4741a7bcdb746d716b3", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000012D274", "ncbi_nrp_id": "WP_006688844.1", "uniref100_id": "UniRef100_Q9PQN8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D274", "RefSeq:WP_006688844.1", "UniRef:UniRef100_Q9PQN8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQN8", "uniref90_id": "UniRef90_Q9PQN8" }, "psc": { "uniref90_id": "UniRef90_Q9PQN8", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q6F1X1", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q6F1X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6F1X1" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "GPKLONFDEH_515", "locus": "GCA000006625_00482" }, { "type": "cds", "sequence": "contig_1", "start": 288759, "stop": 288872, "strand": "+", "frame": 3, "gene": "rpmJ", "product": "50S ribosomal protein L36", "db_xrefs": [ "COG:COG0257", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_006688931.1", "SO:0001217", "UniParc:UPI0000134041", "UniRef:UniRef100_Q9PQN7", "UniRef:UniRef50_Q85FI9", "UniRef:UniRef90_Q9PQN7" ], "nt": "ATGAAAGTTAGAGCTTCAGTTAAAGCAATTTGTAAAGATTGCAAAATCGTAAAGCGATCAGGTGTTGTGCGTGTTATTTGTGCTAATTCAAAACACAAACAACGTCAAGGTTAA", "aa": "MKVRASVKAICKDCKIVKRSGVVRVICANSKHKQRQG", "aa_hexdigest": "d28a9c087bfea917966c78080d770074", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000134041", "ncbi_nrp_id": "WP_006688931.1", "uniref100_id": "UniRef100_Q9PQN7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134041", "RefSeq:WP_006688931.1", "UniRef:UniRef100_Q9PQN7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQN7", "uniref90_id": "UniRef90_Q9PQN7" }, "psc": { "uniref90_id": "UniRef90_Q9PQN7", "gene": "rpmJ", "product": "50S ribosomal protein L36", "uniref50_id": "UniRef50_Q85FI9", "cog_id": "COG0257", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "genes": [ "rpmJ" ], "id": "GPKLONFDEH_516", "locus": "GCA000006625_00483" }, { "type": "cds", "sequence": "contig_1", "start": 288897, "stop": 289295, "strand": "+", "frame": 3, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "COG:COG0099", "COG:J", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "RefSeq:WP_006688863.1", "SO:0001217", "UniParc:UPI00000C1BEC", "UniRef:UniRef100_Q9PQN6", "UniRef:UniRef50_Q9PQN6", "UniRef:UniRef90_Q9PQN6" ], "nt": "ATGGCACGTATTTTAGGGGTAGATATTCCTAATGATAAAAGAGTTGTAATTTCATTAACATACATTTTTGGTATTGGAAAATCTACATCTCAACAAATTTTAAAATTAGCTAATATAGATGAAAATATTCGTGTAAAGGATTTAGCTGATGAACAAATTGCTGAAATTCGTCGAGTTGCTTTAAATTTTGTTAAAGCAAATGGCGAAAAATTACAATTAGAAGGTGATTTACGAAGAACTGTAGCCATGGATATTAAGCGTTTAATGGAAATCGGTTCATATCGTGGAATCCGTCACCGTCGTGGTTTACCAGTGCGTGGACAAAGAACAAAAACAAACGCTCGTACACGTAAAGGACCACGTAAAACAGTTGCTAATAAAAAGATTGAAACTAGATAA", "aa": "MARILGVDIPNDKRVVISLTYIFGIGKSTSQQILKLANIDENIRVKDLADEQIAEIRRVALNFVKANGEKLQLEGDLRRTVAMDIKRLMEIGSYRGIRHRRGLPVRGQRTKTNARTRKGPRKTVANKKIETR", "aa_hexdigest": "f0b55fcf5b9f591bcb04bff715717f28", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1BEC", "ncbi_nrp_id": "WP_006688863.1", "uniref100_id": "UniRef100_Q9PQN6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BEC", "RefSeq:WP_006688863.1", "UniRef:UniRef100_Q9PQN6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQN6", "uniref90_id": "UniRef90_Q9PQN6" }, "psc": { "uniref90_id": "UniRef90_Q9PQN6", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_Q9PQN6", "cog_id": "COG0099", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQN6" ], "product": "Small ribosomal subunit protein uS13" }, "genes": [ "rpsM" ], "id": "GPKLONFDEH_517", "locus": "GCA000006625_00484" }, { "type": "cds", "sequence": "contig_1", "start": 289317, "stop": 289427, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGAAGAAAAAACTTAGTTTTACTAATGGAATTGCATATATTCATGCAACTAAAAATAATACGATTATTACTTTGGCTGATGAACAAGGAAGCGTTTTATCTTGA", "aa": "MAKKKKLSFTNGIAYIHATKNNTIITLADEQGSVLS", "aa_hexdigest": "f49a0d83f0b36385a1968d9643fa6c10", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3907.4934000000003, "isoelectric_point": 9.699671363830568 }, "id": "GPKLONFDEH_518", "locus": "GCA000006625_00485" }, { "type": "cds", "sequence": "contig_1", "start": 289524, "stop": 289682, "strand": "+", "frame": 3, "gene": "rpsK", "product": "30S ribosomal protein S11", "db_xrefs": [ "COG:COG0100", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02948", "SO:0001217", "UniRef:UniRef50_Q46451", "UniRef:UniRef90_Q9PQN5" ], "nt": "ATGGGATTAAAATCTGTTGAAGTTCATGTTAATGGAACAGGTGCAAGTCGTGATACAGCAATTCGTAGCCTACAAGCAGCAGGTTTAGAAGTAACAAAAATTAAAGATGTTACTCCAATTCCACATAATGGGTGTCGTCCACCTAAAAAACCAAGATAG", "aa": "MGLKSVEVHVNGTGASRDTAIRSLQAAGLEVTKIKDVTPIPHNGCRPPKKPR", "aa_hexdigest": "c8589cc617200bf5133cdf178b883ee6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003341A311", "query_cov": 0.9615384615384616, "subject_cov": 0.9803921568627451, "identity": 0.68, "score": 75.9, "evalue": 2.6e-17, "valid": false, "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_A0A1V5HTP7" }, "genes": [ "rpsK" ], "truncated": "5-prime", "pseudogene": { "start": 289317, "stop": 289682, "observations": { "mutation": [ 289425 ], "stop": [ 289425 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQN5", "query_cov": 1.0, "subject_cov": 0.4297520661157025, "identity": 1.0, "score": 108.0, "evalue": 2.7e-29, "gene_start": 1, "gene_end": 52, "reference_start": 70, "reference_end": 121, "reference_sequence": "MAKKKKLSFTNGIAYIHATKNNTIITLADEQGSVLSWASSGSIGYKGTKKKTPYSAGIAAEAAAKAVIDMGLKSVEVHVNGTGASRDTAIRSLQAAGLEVTKIKDVTPIPHNGCRPPKKPR" }, "paralog": false, "identity": 0.9917355371900827, "score": 235.0, "evalue": 6.81e-82, "description": "Internal stop codon at 289425. Nonsense mutation around 289425.", "psc": { "uniref90_id": "UniRef90_Q9PQN5", "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_Q46451", "cog_id": "COG0100", "cog_category": "J", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQN5", "UniRef:UniRef50_Q46451", "COG:COG0100", "COG:J", "KEGG:K02948", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q46451", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q46451" ], "product": "Small ribosomal subunit protein uS11" } }, "id": "GPKLONFDEH_519", "locus": "GCA000006625_00486" }, { "type": "cds", "sequence": "contig_1", "start": 289719, "stop": 290042, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01193.29" ], "nt": "ATGCGTAAATTTTTAAAATACCAACTTGATGTGCCATCAATTAATAGCGAAGACAAAAATCGTACAGTTGTTAAAATTGCCCCACTTGAAATTGGTTTTGGTGACACATTAGGAAATGCCTTACGTCGAATTTGTTTATCATCAATTCCTGGTGCCTCAATGTTCGCTGTTAAATTTGGGGGATATTCGCATGAATTTCAACCATACGAAGGTGTTAAAGAAGACATTACTCACATCATTTTAAACTTAAAAAATTTAGCAATTAAAATTGATGAATTAATTTATAGTGAAGACTATTTCAACAATTTATTAATTGATAAATGA", "aa": "MRKFLKYQLDVPSINSEDKNRTVVKIAPLEIGFGDTLGNALRRICLSSIPGASMFAVKFGGYSHEFQPYEGVKEDITHIILNLKNLAIKIDELIYSEDYFNNLLIDK", "aa_hexdigest": "78b9b1fc7bd3960ded08d074ca95e8d8", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A8S0FJM5", "query_cov": 0.822429906542056, "subject_cov": 0.9166666666666666, "identity": 0.5, "score": 80.5, "evalue": 1.06e-17, "valid": false, "product": "DNA-directed RNA polymerase subunit alpha", "uniref50_id": "UniRef50_A0A3P8JBX3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01193.29", "name": "RNA_pol_L", "length": 68, "aa_cov": 0.616822429906542, "hmm_cov": 0.8970588235294118, "evalue": 1.0452149654921177e-11, "score": 40.00569534301758, "start": 28, "stop": 93 } ], "seq_stats": { "molecular_weight": 12166.917099999988, "isoelectric_point": 6.081681251525878 }, "id": "GPKLONFDEH_520", "locus": "GCA000006625_00487" }, { "type": "cds", "sequence": "contig_1", "start": 290049, "stop": 290702, "strand": "+", "frame": 3, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_Q9PQN4", "UniRef:UniRef90_Q9PQN4" ], "nt": "ATGAAAATTAATTTCAAAGGACCTGGTGTGATTACAGCTAAAGATATTGTATGTCCAGTAGGTTTTGAAATTGTTAACCAAGACTTATATATTGCAGAAGTAACAAAACCAATTGATGTAGAAATTGAAATTTTTGCAAAAACAGGACGTGGTCGTGTCGATTTTAATACAAATAAGGATTTTGTTTCAACACTACATATTATCGCAACCGATTCAAATTATTCACCAGTTCTACATTACGCTTATAATGTAGAAATGATTAAAGATTCAAAATCATCAATGTCTGAGATTTTAACAATTGACATTGCAACAAATGGTACGATATCAGGAAGCGAAGCAATTGCGATTGCTGCTAAGATTATGCAAGCGCATTTAGAACCAATCGTGAACATTGATAAAACAATTAATGAAATGATTATTATGCGTGAGCGTGAGGAAGAAGAAAAACGTCAAAACGCTTCAATTTCAATTGATGATTTAGATTTAACGGTGCGTGCATATAATGCTTTAAAACAATCAGGTATTAATACCACTGCTGAATTAATTGAATTAACAAAATCACAACTTGAAAAAATTAAGAATTTAGGACGTAAATCAGTTACTGAAATTATTCAAAAATTAACTGAACGTTCACTTGAGTTAAAAAAAGATTAA", "aa": "MKINFKGPGVITAKDIVCPVGFEIVNQDLYIAEVTKPIDVEIEIFAKTGRGRVDFNTNKDFVSTLHIIATDSNYSPVLHYAYNVEMIKDSKSSMSEILTIDIATNGTISGSEAIAIAAKIMQAHLEPIVNIDKTINEMIIMREREEEEKRQNASISIDDLDLTVRAYNALKQSGINTTAELIELTKSQLEKIKNLGRKSVTEIIQKLTERSLELKKD", "aa_hexdigest": "9ad8f1f32d9374f340e0dd7a40f78f74", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoA" ], "truncated": "5-prime", "pseudogene": { "start": 289749, "stop": 290702, "observations": { "mutation": [ 290040 ], "stop": [ 290040 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQN4", "query_cov": 1.0, "subject_cov": 0.6636085626911316, "identity": 1.0, "score": 406.0, "evalue": 3.32e-141, "gene_start": 1, "gene_end": 217, "reference_start": 111, "reference_end": 327, "reference_sequence": "MRKFLKYQLDVPSINSEDKNRTVVKIAPLEIGFGDTLGNALRRICLSSIPGASMFAVKFGGYSHEFQPYEGVKEDITHIILNLKNLAIKIDELIYSEDYFNNLLIDKWPKMKINFKGPGVITAKDIVCPVGFEIVNQDLYIAEVTKPIDVEIEIFAKTGRGRVDFNTNKDFVSTLHIIATDSNYSPVLHYAYNVEMIKDSKSSMSEILTIDIATNGTISGSEAIAIAAKIMQAHLEPIVNIDKTINEMIIMREREEEEKRQNASISIDDLDLTVRAYNALKQSGINTTAELIELTKSQLEKIKNLGRKSVTEIIQKLTERSLELKKD" }, "paralog": false, "identity": 0.9968454258675079, "score": 611.0, "evalue": 1.64e-224, "description": "Internal stop codon at 290040. Nonsense mutation around 290040.", "psc": { "uniref90_id": "UniRef90_Q9PQN4", "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_Q9PQN4", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQN4", "EC:2.7.7.6", "UniRef:UniRef50_Q9PQN4", "COG:COG0202", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQN4" ], "product": "DNA-directed RNA polymerase subunit alpha" } }, "id": "GPKLONFDEH_521", "locus": "GCA000006625_00488" }, { "type": "cds", "sequence": "contig_1", "start": 290775, "stop": 291089, "strand": "+", "frame": 3, "gene": "rplQ", "product": "50S ribosomal protein L17", "db_xrefs": [ "COG:COG0203", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02879", "SO:0001217", "UniRef:UniRef50_P47424", "UniRef:UniRef90_Q9PQN3" ], "nt": "ATGGTGAGTCGTCAACAAGTATCTGATGTTATAAGTCATGGTTCAATTGTGACAACAAAAACAAAAGCAAAAGAATCACAACGTCATGTTGATCATTTAATCACTCTTGCTAAGAAAAACACACTTGCTTCACGTCGTGCAGCAGCTGCGATTTTATTAGGAACAAATCAACACTCAGCGGATGATTTATTACGTAAATTATTTAACGAATTAGGTCCTAAGTATGCTAATCGTGCTGGTGGATACACTCGTGTTATTAAACTTGGTAATCGTCCAGGAGATAATACAGAAGAAGCTGTTTTACAATTAGTTTAG", "aa": "MVSRQQVSDVISHGSIVTTKTKAKESQRHVDHLITLAKKNTLASRRAAAAILLGTNQHSADDLLRKLFNELGPKYANRAGGYTRVIKLGNRPGDNTEEAVLQLV", "aa_hexdigest": "5bdcf156fcc3b61db1024f562859be8f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQN3", "query_cov": 1.0, "subject_cov": 0.8739495798319328, "identity": 1.0, "score": 200.0, "evalue": 8.47e-65, "valid": true, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P47424", "cog_id": "COG0203", "cog_category": "J", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47424" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [ "rplQ" ], "id": "GPKLONFDEH_522", "locus": "GCA000006625_00489" }, { "type": "cds", "sequence": "contig_1", "start": 291356, "stop": 291733, "strand": "+", "frame": 2, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E28CBB8", "UniRef:UniRef90_UPI001E28CBB8" ], "nt": "ATGAATAAACAAAAAAGAAAAATCTCAGTATTAACTTCAATATCATTATTTTTAATTATTAGTTTAAGTATTGGGTTAGGTTTGGGATTAATAAAAAATAATGAAAATTCCATCACTTCTAAAGTTCAAAAAAGTAGCAATTTTTTTATTAATGATTTATCAAAACAATTGTTTTTAGATGTTTTTAATCAAGATAAATTAGAATTAAATGCTTATATGTATGATCAAAATAATGTATCAGATCAACAATTATTAGATTTAAAATTTGCTTTAACATTATTAAATCCTAACAGATTAGATATTAATGATGACGATACAATTGCCATTATGAAAAATAATGCTAAAAAAATTATTTTTCAAAGTTTATCTAATAATTGA", "aa": "MNKQKRKISVLTSISLFLIISLSIGLGLGLIKNNENSITSKVQKSSNFFINDLSKQLFLDVFNQDKLELNAYMYDQNNVSDQQLLDLKFALTLLNPNRLDINDDDTIAIMKNNAKKIIFQSLSNN", "aa_hexdigest": "d2e5c49cfa2d17e372ed4c72789b8176", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 291356, "stop": 292034, "observations": { "insertion": [ 291992 ], "mutation": [ 291731, 291737 ], "stop": [ 291731, 291737 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E28CBB8", "query_cov": 1.0, "subject_cov": 0.5387931034482759, "identity": 1.0, "score": 204.0, "evalue": 1.62e-64, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MNKQKRKISVLTSISLFLIISLSIGLGLGLIKNNENSITSKVQKSSNFFINDLSKQLFLDVFNQDKLELNAYMYDQNNVSDQQLLDLKFALTLLNPNRLDINDDDTIAIMKNNAKKIIFQSLSNNWYWLFTNLKSLEFSYTPYKPNFYAPYINEKQDFTKSSIFDKVININKPLTNYIKIKQKLFDKENYRSVDVYYLQFGDHLILRLLKFNKKIILMFDLIQIFFNSINQL" }, "paralog": false, "identity": 0.9911111111111112, "score": 397.0, "evalue": 4.52e-143, "description": "Internal stop codon at 291731, 291737. Frameshift due to insertion around 291992. Nonsense mutation around 291731, 291737.", "psc": { "uniref90_id": "UniRef90_UPI001E28CBB8", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI001E28CBB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E28CBB8", "UniRef:UniRef50_UPI001E28CBB8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E28CBB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E28CBB8" ], "product": "aromatic motif membrane protein" } }, "id": "GPKLONFDEH_523", "locus": "GCA000006625_00490" }, { "type": "cds", "sequence": "contig_1", "start": 291947, "stop": 292789, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYW8", "UniRef:UniRef90_A0A2C9DYW8" ], "nt": "TTGCAATTTGGTGATCACTTAATCTTACGTTTATTAAAATTTAATAAAAAAAATCATTTTGATGTTCGATTTGATACAGATCTTTTTCAATTCAATAAACCAATTATAAATTTGGAATTAATAGCAAAAGAAATGCAAACAAGTATTGATTATCATCGAGAAGATAATTTTAAAAAAGATATTTTAGATTATAAAAAAAATTATGAAGATTATTTATATTATGATGAAAAGCTTAACTCAAGTATTTATCAACAGGGATTAGATAAAATAAATAAAGAATATTATGAAGTCAATAATTTTAATTTTTTTAATACTAAAAAACCACTTTTTCAAAAATTTAGTTATCAAATGTTTTTAGAATTAAAAGATAAGTTGGGTTTTAAACGTTATGTTTTAAGAAATATTGATCAAAAAGAAGTTAGAGCACGTGATCATTTTAATGACAATGAAAAAAAACAACAAAATAAGAATTATTTTAGATCTTTTGATAAAGAATTGAATTATTTTCAAAAATATATTGATCATAATTTAGATATTAATATTAATAAAATTTCAAAGGAAGCATATATTACACAAAAAATAATTAAAAATCTTTTATTAGATGCTTTCAATAATAATGATGTTCAAATTACAAAATATCTAATTCAACAAAATGATAAGGAATACCAAAATAAATTAATTAAACAATTAATAGAATTTAGTAATTATTTTAAAAACCAAAAAACACCAAGAGATTCTAATGAATATAAGCAAAAATTAAATGATTATGAAAAAATATTTTCACAAAATTGGTATTTTATTTTAATGAATTTAAAGCACTTTGAATTAAGTTTTAATAATTGA", "aa": "MQFGDHLILRLLKFNKKNHFDVRFDTDLFQFNKPIINLELIAKEMQTSIDYHREDNFKKDILDYKKNYEDYLYYDEKLNSSIYQQGLDKINKEYYEVNNFNFFNTKKPLFQKFSYQMFLELKDKLGFKRYVLRNIDQKEVRARDHFNDNEKKQQNKNYFRSFDKELNYFQKYIDHNLDININKISKEAYITQKIIKNLLLDAFNNNDVQITKYLIQQNDKEYQNKLIKQLIEFSNYFKNQKTPRDSNEYKQKLNDYEKIFSQNWYFILMNLKHFELSFNN", "aa_hexdigest": "e920b36545e522cce1e63568d2e0a5df", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 291647, "stop": 293092, "observations": { "mutation": [ 291731, 291737, 292787 ], "stop": [ 291731, 291737, 292787 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYW8", "query_cov": 1.0, "subject_cov": 0.449438202247191, "identity": 0.996, "score": 548.0, "evalue": 1.23e-191, "gene_start": 1, "gene_end": 280, "reference_start": 198, "reference_end": 477, "reference_sequence": "MNKQKRKISVLTSISLFLIISLSIGLGLGLIKNNENSITSKVQKSSNFFINDLSKQLFLDVFNQDKLELNAYMYDQNNVSDQQLLDLKFALTLLNPNRLDINDDDTIAIMKNNAKKIIFQSLSNNWYWLFTNLKSLEFSYTPYKPNFYAPYINEKQDFTKSSIFDKVININKPLTNYIKIKQKLFDKENYRSVDVYYLQFGDHLILRLLKFNKKNHFDVRFDTDLFQFNKPIINLELIAKEMQTSIDYHREDNFKKDILDYKKNYEDYLYYDEKLNSSIYQQGLDKINKEYYEVNNFNFFNTKKPLFQKFSYQMFLELKDKLGFKRYVLRNIDQKEVRARDHFNDNEKKQQNKNYFRSFDKELNYFQKYIDHNLDININKISKEAYITQKIIKNLLLDAFNNNDVQITKYLIQQNDKEYQNKLIKQLIEFSNYFKNQKTPRDSNEYKQKLNDYEKIFSQNWYFILMNLKHFELSFNNWFTLPKQINPITKEMLGPSEKYLKRLKKLKPYEDYYFINNYLEKKQEGDTSMLIGSFKDLYITKNQSIFNIKIDFSSSENRLSLNPLIYFFPKTKNKLSVDLITSIFHQAIFHQNKEYYQLFETDMIDKYEYGIPAQCYFYEGNSN" }, "paralog": false, "identity": 0.9937629937629938, "score": 969.0, "evalue": 0.0, "description": "Internal stop codon at 291731, 291737, 292787. Nonsense mutation around 291731, 291737, 292787.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYW8", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A2C9DYW8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYW8", "UniRef:UniRef50_A0A2C9DYW8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYW8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYW8" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_524", "locus": "GCA000006625_00491" }, { "type": "cds", "sequence": "contig_1", "start": 292829, "stop": 293212, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGACCAAGTGAAAAATATTTAAAAAGGTTAAAAAAACTTAAACCATATGAGGATTATTATTTCATTAATAATTATTTAGAAAAAAAACAAGAAGGAGATACTTCAATGTTGATTGGTTCATTTAAGGATTTATATATTACAAAAAATCAATCTATTTTTAATATTAAGATCGATTTTTCAAGTAGTGAAAACAGATTAAGTTTAAATCCTTTGATTTATTTTTTTCCTAAAACTAAAAATAAATTATCAGTTGATTTAATCACATCAATTTTTCATCAAGCTATATTTCACCAAAATAAAGAATACTATCAATTGTTTGAAACTGATATGATTGATAAATATGAATATGGTATCCCAGCCCAAATGTTACTTTTATGA", "aa": "MLGPSEKYLKRLKKLKPYEDYYFINNYLEKKQEGDTSMLIGSFKDLYITKNQSIFNIKIDFSSSENRLSLNPLIYFFPKTKNKLSVDLITSIFHQAIFHQNKEYYQLFETDMIDKYEYGIPAQMLLL", "aa_hexdigest": "f3f9604ec496aa79b5269033fb9e5502", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15191.396299999988, "isoelectric_point": 8.614668464660646 }, "id": "GPKLONFDEH_525", "locus": "GCA000006625_00492" }, { "type": "cds", "sequence": "contig_1", "start": 293228, "stop": 293680, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTTTTTTTAAAAAAAATCATCTACTAAAATTAAGTTTATTTTTTATTCCTCTAACATTAATTTCAATCCTTATCACCTCATGTAGCACATCAAATTTAAAAAAACAACAGATATTAACAAATAATGAAATTAGTTTTTTTATGGATAAATCCCAAAATTGTTATGTTGGTTTAATTGTTGATCAGACATTAAAAAATGATAAAAAAACTATTTTTAACGTTAGTTTAATTGATGAAAAAAATACGAAGTTAAATTTATTAGTTAAAGATTATCAAATTAAAGATAACAAAATTTTAATTAGATTACCGCGTTTAATAAAACTTAATGATCATTTAATTATTACAAGCAATAATGCTTCATTCATACCTATTAGAAAAATAATTAATATTGATAATTTAATTGATTTGAATATAATCAGAACAAATAATCAAACACAAGATTCTTGA", "aa": "MFIFFKKNHLLKLSLFFIPLTLISILITSCSTSNLKKQQILTNNEISFFMDKSQNCYVGLIVDQTLKNDKKTIFNVSLIDEKNTKLNLLVKDYQIKDNKILIRLPRLIKLNDHLIITSNNASFIPIRKIINIDNLIDLNIIRTNNQTQDS", "aa_hexdigest": "13b4d6bd2b258b879b95f67fa7c056e0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17437.372799999994, "isoelectric_point": 9.628240394592286 }, "id": "GPKLONFDEH_526", "locus": "GCA000006625_00493" }, { "type": "cds", "sequence": "contig_1", "start": 293702, "stop": 293812, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAATAATCTTTTAATAACTATTTTTCCTAATAAAAAAGACCGTGATGAATATATTAAATCACAACAAGAAATTAAAATAGAATATGCTCATGAAATAGCGAATTGA", "aa": "MINNLLITIFPNKKDRDEYIKSQQEIKIEYAHEIAN", "aa_hexdigest": "4c324ac8d1e1c54a54dbc970d47787ec", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4333.9159, "isoelectric_point": 5.596389961242674 }, "id": "GPKLONFDEH_527", "locus": "GCA000006625_00494" }, { "type": "cds", "sequence": "contig_1", "start": 293831, "stop": 293983, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAATGATGCTAATAAAGCAGCAGTATTTAATGAAAATACAGCTAGAAGTAAACTTAATAACAATCTTTTTAATAATGTATCATTAAGCAATCCATTTGCTTATGAACAAGCACGTAAGTTTCACAATACATTATTTAATAAAAATTGA", "aa": "MQNDANKAAVFNENTARSKLNNNLFNNVSLSNPFAYEQARKFHNTLFNKN", "aa_hexdigest": "95df408a747d199f4db0f4ac84666e1b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5761.275700000001, "isoelectric_point": 9.819775962829588 }, "id": "GPKLONFDEH_528", "locus": "GCA000006625_00495" }, { "type": "cds", "sequence": "contig_1", "start": 294020, "stop": 294331, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATATCCTGATGATAATTTATTTCAAGAATCGAGCGAGGAAACAGCAGAAGGATTAAAAGATTCAAAAATCAATAATCGTAGTTCTTTTTATCGTGCTCAATCGAATGAATTTATTGATGGGTTATATGTTGTAGAAAGTTCTAATTTAAATAAAAAACCAGAAGAATTAGATGAATATACTGATTTTAGTAAAACAGCTAAATTTACTCTGTTGAATAATGAAGGTTTTGTTTTTACAATCTCCATTGAAGAAAATTATGATAAAACCAAAAAACTAATTTCGCGTAAAGTTAGTTTGTTGCCATGA", "aa": "MLYPDDNLFQESSEETAEGLKDSKINNRSSFYRAQSNEFIDGLYVVESSNLNKKPEELDEYTDFSKTAKFTLLNNEGFVFTISIEENYDKTKKLISRKVSLLP", "aa_hexdigest": "bc54aabfc2040e377a830c65187c3e79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11918.065399999983, "isoelectric_point": 4.662582206726075 }, "id": "GPKLONFDEH_529", "locus": "GCA000006625_00496" }, { "type": "cds", "sequence": "contig_1", "start": 294353, "stop": 294523, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGATCATGTAATAAAACAAGAATTTAATTTAGCTAATTATACTGAAAATTCATACGATTACAATGCTGTTAATTTTCATTCACCTCTTGAAATTAAAGTTTATGAAGATCAATTTGGCGGAAAAGCAATTCGTTTTTCATTTGTTGATATTGATCCTAATTAA", "aa": "MFNDHVIKQEFNLANYTENSYDYNAVNFHSPLEIKVYEDQFGGKAIRFSFVDIDPN", "aa_hexdigest": "d2aa4f3fb73b765b17fc08eef5d8abf4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6606.1698000000015, "isoelectric_point": 4.547426414489746 }, "id": "GPKLONFDEH_530", "locus": "GCA000006625_00497" }, { "type": "cds", "sequence": "contig_1", "start": 294590, "stop": 294847, "strand": "+", "frame": 2, "gene": null, "product": "DUF4231 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6H3", "UniRef:UniRef90_A0AAC9X6H3" ], "nt": "ATGAACAATTACATACAGCAAGTTATTGATTATGTTAATGATAAAACCTATAAAAATAAGACATATTTTATAAAGAAATATGCATTTGCTTTTTTGACTTCATTAATAATTTTTATTTTTTTATTAGTATTTGGTTTATCAAATTATTTTAATCCTTTGGTTAGTTTAGTTGGTGTTGTATTAATTTTTAGTGTTTTAGTTGTCTTTTGTATTCATATAAAAATTATCAATAATTATAGTTTAAATTTATTAAAATGA", "aa": "MNNYIQQVIDYVNDKTYKNKTYFIKKYAFAFLTSLIIFIFLLVFGLSNYFNPLVSLVGVVLIFSVLVVFCIHIKIINNYSLNLLK", "aa_hexdigest": "400f7ff27682497c1f850f43a5329eb6", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 294590, "stop": 294997, "observations": { "mutation": [ 294845 ], "stop": [ 294845 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6H3", "query_cov": 1.0, "subject_cov": 0.6296296296296297, "identity": 1.0, "score": 157.0, "evalue": 5.35e-48, "gene_start": 1, "gene_end": 85, "reference_start": 1, "reference_end": 85, "reference_sequence": "MNNYIQQVIDYVNDKTYKNKTYFIKKYAFAFLTSLIIFIFLLVFGLSNYFNPLVSLVGVVLIFSVLVVFCIHIKIINNYSLNLLKWLRNQKELSYKYIINILDFILNKQLYLNLTIIEVQQICIDLEEFKKTNVI" }, "paralog": false, "identity": 0.9925925925925926, "score": 262.0, "evalue": 5.78e-92, "description": "Internal stop codon at 294845. Nonsense mutation around 294845.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6H3", "product": "DUF4231 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6H3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6H3", "UniRef:UniRef50_A0AAC9X6H3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6H3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6H3" ], "product": "DUF4231 domain-containing protein" } }, "id": "GPKLONFDEH_531", "locus": "GCA000006625_00498" }, { "type": "cds", "sequence": "contig_1", "start": 294987, "stop": 295574, "strand": "+", "frame": 3, "gene": "yIH1", "product": "Impact N-terminal domain-containing protein", "db_xrefs": [ "COG:COG1739", "COG:R", "RefSeq:WP_010891715.1", "SO:0001217", "UniParc:UPI00000C1BF1", "UniRef:UniRef100_A0A2C9DY85", "UniRef:UniRef50_A0A2C9DY85", "UniRef:UniRef90_A0A2C9DY85" ], "nt": "ATGTCATTTAAAACAATTGCATATACCCAAAAAAATATAAAAATTATTAAAAAATCAAAATTTATAACGATCGTTGAACCTATTAGCGATAAAAATGAAGTTCAATCATTATTTAAAAAATATAAAGAACAATATTACGATGCAACGCACATTTGTTTTGCTTATGTGATAGGTCGTGAATTTAATTATATGAAAGCATATGATGATGGTGAACCAAATGGAACAGCTGGAATACCTATTTTAAATATCATTAAGATCAAATCATTAACCAATGTGATTATATTTGTTGTACGTTATTTTGGTGGCATTAAATTAGGCACTGGAGGATTAATTCGTGCTTATTCACAATGTGCTAAAGAAATTATAGATAACTGTGAAATAATAATTTTACAAAAATATTTTTACTATAAAGTTGTTTATAAGCTAAAAAATACTAAATTTGCAAAGCAAATTTTAACATCAATTAGTTATGAAAAACTAATAGAGAGTTATGAAGATGATAGAGTTATTTTAAAAATCCGAATTAACACCAAAATTAATATATTTATGTCTAAGATTGAATTTATTTTTATAAAACATGATTATTAA", "aa": "MSFKTIAYTQKNIKIIKKSKFITIVEPISDKNEVQSLFKKYKEQYYDATHICFAYVIGREFNYMKAYDDGEPNGTAGIPILNIIKIKSLTNVIIFVVRYFGGIKLGTGGLIRAYSQCAKEIIDNCEIIILQKYFYYKVVYKLKNTKFAKQILTSISYEKLIESYEDDRVILKIRINTKINIFMSKIEFIFIKHDY", "aa_hexdigest": "b923c77a51ef3e6b36427335a71ace41", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1BF1", "ncbi_nrp_id": "WP_010891715.1", "uniref100_id": "UniRef100_A0A2C9DY85", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BF1", "RefSeq:WP_010891715.1", "UniRef:UniRef100_A0A2C9DY85" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DY85", "uniref90_id": "UniRef90_A0A2C9DY85" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DY85", "gene": "YIH1", "product": "Impact N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DY85", "cog_id": "COG1739", "cog_category": "R" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DY85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY85" ], "product": "Impact N-terminal domain-containing protein" }, "genes": [ "yIH1" ], "id": "GPKLONFDEH_532", "locus": "GCA000006625_00499" }, { "type": "cds", "sequence": "contig_1", "start": 295582, "stop": 295713, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATATTAACAAAACAAGTTCCTAATCATTTAAAAAATGAATATGATACAGCAAGATTGTTTTTTAGAATAGAGAGAACAATTGGTGAAATATGTAAAAAATACGCGTTAACTAATCCTTTAGAGTAA", "aa": "MFILTKQVPNHLKNEYDTARLFFRIERTIGEICKKYALTNPLE", "aa_hexdigest": "47048d3c8b54112e7098339f0ede6833", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5155.990499999999, "isoelectric_point": 8.978334617614745 }, "id": "GPKLONFDEH_533", "locus": "GCA000006625_00500" }, { "type": "cds", "sequence": "contig_1", "start": 296470, "stop": 296805, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAATCATACGATGGTCTTTATAAAAAAACAATTAATGATGAAGCTAATAAAATCAAAAATATTAAATCTGATGTTAAAAATTTAAAACATAATTATTTTGAAAAAATTGATAATGGTGAAGAATATTATAAGTTATACAATAGAGAAATTATGGAATTATTTCATGAAGCTAATCAACTTCATGAAAATATTTCTTCAGTACTACAGAGTATATTAAACAGAAAATTAATTGAAAATAAAATCGATAATATTAAAAGAGAAATCCAAGAACATAAAAAAGTTTCACAATATATTAAACAAAATGGCCACAAAAATAATCAAGATTTTTGA", "aa": "MIESYDGLYKKTINDEANKIKNIKSDVKNLKHNYFEKIDNGEEYYKLYNREIMELFHEANQLHENISSVLQSILNRKLIENKIDNIKREIQEHKKVSQYIKQNGHKNNQDF", "aa_hexdigest": "1cebf02797ce84eac79ca53153dc5d6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13399.938099999985, "isoelectric_point": 7.893525123596191 }, "id": "GPKLONFDEH_534", "locus": "GCA000006625_00501" }, { "type": "cds", "sequence": "contig_1", "start": 296995, "stop": 297366, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAAATTTTAAAAGAACTTTTTTATTATCATTTATAGGAGTTATAGTTACACCAATTCCTATAATTTTGAGTTGCTCATTAACAAAAATTAAAAAAGAAGACAATAAAAATTTCTTTAATCTTTATTTGAAAATTAAAGAAGAAGCTAGTTCTAAAGTTGATTTTGTTCTTAAAAATAATCATAAATACAATTATCTTCTTAATAAAGCAGATATAATCAATAATTATATTAATTACTTGGTGAATTTAAATTTAAATGAAAATAGTGAGAATGAATTATTAGCATTTACTTATTATGAATTATATAAATTAATAAAAGATTTTAATTATCTTTTAAAAGAAAAAACACAAGAATATGAATCATGA", "aa": "MKINFKRTFLLSFIGVIVTPIPIILSCSLTKIKKEDNKNFFNLYLKIKEEASSKVDFVLKNNHKYNYLLNKADIINNYINYLVNLNLNENSENELLAFTYYELYKLIKDFNYLLKEKTQEYES", "aa_hexdigest": "19ef68992141b2daa8e1dbdf71784603", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14694.880399999982, "isoelectric_point": 8.821740913391114 }, "id": "GPKLONFDEH_535", "locus": "GCA000006625_00502" }, { "type": "cds", "sequence": "contig_1", "start": 298408, "stop": 298695, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGGAGATGGTGTCAATGATGCACCTGCACTAAAAGCTGCTGACATTGGATGCGCAATGGGTATAACTGGAACTGATGTTTCAAAAGCATCAAGTGATATGATATTAATTGATGATAATTTCGCAACAATTATTAATGCTGTTAGTTTAGGACGTTCTATTATGGACAACATTAAACGTATTATTGTTTTATTATTGATCACTAATTTATCTGGATTAGTTTCATTAATATTTGGAATTATTATTCTAGGAATTAATCCTATGTCATCACTACAAATATTATGA", "aa": "MTGDGVNDAPALKAADIGCAMGITGTDVSKASSDMILIDDNFATIINAVSLGRSIMDNIKRIIVLLLITNLSGLVSLIFGIIILGINPMSSLQIL", "aa_hexdigest": "f86b3d2e3c8f6c036c74f796dfbea6bc", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI002964F5A5", "query_cov": 1.0, "subject_cov": 0.8407079646017699, "identity": 0.516, "score": 86.7, "evalue": 4.04e-20, "valid": false, "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_A0A7R9ZHY7" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9844.57399999999, "isoelectric_point": 4.306258583068849 }, "id": "GPKLONFDEH_536", "locus": "GCA000006625_00503" }, { "type": "cds", "sequence": "contig_1", "start": 298720, "stop": 299223, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGAGTGTTGTTGTAAAACATCAAAGTGATTATTATTTAATTACTAAAGGAGCAATTGATGCTATTGACAAAATTGTTCAAAATCCAATTACAAAAGATGTTTATGAAGCAAATGATTTTTTAAGTAAACAAGCTTTAAGAATTTTATGTGTTGCAATTAAAAAACTATCATCTATACCAACTAATTTTAACCAAGATGAACTTGAAAAATGTTTAGAATTTGTTGGTCTAATCGGTATGATAGATCCGCCTCGTCCTGAAACACAAGAGGCAGTAAAAATTGCTATTAAAGCTGGAATCCGTCCGATAATGATTACTGGCGATCATATTAATACAGCATCAGCCATTGCTAAACAAGTTGGTATTTTAAACGAAGGACAAGAAGTTTTGAATGGTAATGAATTGAATATGATGAGTGATAATGAATTAATAAATAATATTGATCGTTATAGTGTTTATGCCCGAGTTTCTCCAACAGATAAAATCCGTATTGTTAAAGCTTGA", "aa": "MSVVVKHQSDYYLITKGAIDAIDKIVQNPITKDVYEANDFLSKQALRILCVAIKKLSSIPTNFNQDELEKCLEFVGLIGMIDPPRPETQEAVKIAIKAGIRPIMITGDHINTASAIAKQVGILNEGQEVLNGNELNMMSDNELINNIDRYSVYARVSPTDKIRIVKA", "aa_hexdigest": "1881f377a222ba155a375f1bde1a1ff1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 60, "aa_cov": 0.3473053892215569, "hmm_cov": 0.9666666666666667, "evalue": 1.5213929441038098e-08, "score": 30.824228286743164, "start": 68, "stop": 125 } ], "seq_stats": { "molecular_weight": 18526.2521, "isoelectric_point": 5.714842414855957 }, "id": "GPKLONFDEH_537", "locus": "GCA000006625_00504" }, { "type": "cds", "sequence": "contig_1", "start": 299407, "stop": 299679, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTCATTATCTATAGCAGCTATTCCTGAAGGAATTGTGGCAATTGTAACAATTGTTTTATCATTCGGTATTAAAGAAATGGCAAAACAAAATGCTTTAATTAAACGTTTGCCAGCTGTTGAAACTCTAGGAAGTGCTAATGTTATTTGTTCTGATAAAACAGGAACCTTAACACAAAACAAAATGACTGTTACTAAAGTTTTCACTAATAAATTAAAAATAACAGATTTAATTGATAATGAGAGTGTTTTTAATTTAATTAAATGA", "aa": "MAISLSIAAIPEGIVAIVTIVLSFGIKEMAKQNALIKRLPAVETLGSANVICSDKTGTLTQNKMTVTKVFTNKLKITDLIDNESVFNLIK", "aa_hexdigest": "f15972ef9e26e141da7eeba10e173d16", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9661.411499999993, "isoelectric_point": 9.25155162811279 }, "id": "GPKLONFDEH_538", "locus": "GCA000006625_00505" }, { "type": "cds", "sequence": "contig_1", "start": 299707, "stop": 300561, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "GTGAAAAAAAATAATGAAAATCAAACCGACTCCTTTATGTCATTTGATCCAGAAAATATAAATCCATCAACAGGTCTAAATGATGAGCAGGTTTTAAAAAGTCGTCAAATTTATGGTTTTAATGAAATCAAAAAGAAAAAAAAATCCCACATTATTACAAAATTTTTAAAGCAGTTTTTAGATTTTATGGTTATTTTACTTGTAATCGCTGCTGCTGTTACTTTAGCTTTAGTGATTATTAAACCTCCTCATGATACAGCTGAATTAGTTGTTCAATATGTAGAATTTGGAATAATATGTTTTATTTTATTGTTAAATGCAATTTTTGGTACCATTCAAGAAGTGAAAGCTGAAAAAAATACGGATGCATTGTCTAAATTAGCGTCTCATCAGGTCAAAGTATTACGTAATAATCAAATTAGAATTATTAATTCTAACCAAGTTGTAATGGGTGATGTTTTGATTTTAGAAGCTGGTGATCAAGTCCCAGCTGATGCTTTATTAGTGAACAGCTCATCACTTGAAGTAGATGAAGCTATTTTAACAGGAGAATCTTTACCAGTTGTAAAAAATGCCAAGGCAATTGTTAAAAAAAATACAGTAATTGGCGACTGTTTAAATCAAATTTTTTCTGGAACATATATTACTAATGGAACAGCAAAAGCAATTGTTACTAATATCGGTATGAATACTGAAATTGGTAAAATAGCCAAATTAATTAATGATCAAAAAACTAAATTAACGCCTTTACAAAAAAAAATTAATAAGTTATCTAAAATTATAGGTCTATTTGCTTCTATTTTATGTATTGCAGTATTTATTATTTATATTTATTTAATTGGAGATGGAAATTGA", "aa": "MKKNNENQTDSFMSFDPENINPSTGLNDEQVLKSRQIYGFNEIKKKKKSHIITKFLKQFLDFMVILLVIAAAVTLALVIIKPPHDTAELVVQYVEFGIICFILLLNAIFGTIQEVKAEKNTDALSKLASHQVKVLRNNQIRIINSNQVVMGDVLILEAGDQVPADALLVNSSSLEVDEAILTGESLPVVKNAKAIVKKNTVIGDCLNQIFSGTYITNGTAKAIVTNIGMNTEIGKIAKLINDQKTKLTPLQKKINKLSKIIGLFASILCIAVFIIYIYLIGDGN", "aa_hexdigest": "439de99179d3c4b164eddea2fca56014", "start_type": "GTG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 53, "aa_cov": 0.18661971830985916, "hmm_cov": 1.0, "evalue": 1.1898111910569485e-17, "score": 58.978031158447266, "start": 21, "stop": 73 } ], "seq_stats": { "molecular_weight": 31241.360600000004, "isoelectric_point": 8.93623676300049 }, "id": "GPKLONFDEH_539", "locus": "GCA000006625_00506" }, { "type": "cds", "sequence": "contig_1", "start": 300712, "stop": 301041, "strand": "+", "frame": 1, "gene": null, "product": "UPF0154 protein UU265", "db_xrefs": [ "GO:0016020", "KEGG:K09976", "RefSeq:WP_010891717.1", "SO:0001217", "UniParc:UPI0000139537", "UniRef:UniRef100_Q9PQM6", "UniRef:UniRef50_Q9PQM6", "UniRef:UniRef90_Q9PQM6" ], "nt": "ATGAATTTTTCAAGTTTTATATTTAAAGTTTCTGATGTTTTTAAATCAGTTATTCATGAAGCTTCAGATGTTGTTACTAAAGCAGATTTAGATAATGCTAATGCACATACACATTCATTAGCAGTTGGCTTAGGTATTGGAATTGTTTTATTTTTAATCGCAGGACTTATTATCGGTTATTTTATTTCGATGAAAATTATGAAACGCCAATTGAAGAAGAATCCACCAATTTCAAAGGATACAATTCGTATGATTTATCAACAAGTAGGACGTAAGCCAAGTGAGTCACAAATTAATGAGATTTATAATCGGGCAGTAAAACAAAAATAA", "aa": "MNFSSFIFKVSDVFKSVIHEASDVVTKADLDNANAHTHSLAVGLGIGIVLFLIAGLIIGYFISMKIMKRQLKKNPPISKDTIRMIYQQVGRKPSESQINEIYNRAVKQK", "aa_hexdigest": "e23ed19d391a8ed9a4853c64b1fffc46", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0000139537", "ncbi_nrp_id": "WP_010891717.1", "uniref100_id": "UniRef100_Q9PQM6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139537", "RefSeq:WP_010891717.1", "UniRef:UniRef100_Q9PQM6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQM6", "uniref90_id": "UniRef90_Q9PQM6" }, "psc": { "uniref90_id": "UniRef90_Q9PQM6", "product": "UPF0154 protein UU265", "uniref50_id": "UniRef50_Q9PQM6", "kegg_orthology_id": "K09976", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQM6" ], "product": "UPF0154 protein UU265" }, "genes": [], "id": "GPKLONFDEH_540", "locus": "GCA000006625_00507" }, { "type": "cds", "sequence": "contig_1", "start": 301046, "stop": 301672, "strand": "+", "frame": 2, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872", "RefSeq:WP_006688825.1", "SO:0001217", "UniParc:UPI0000129F33", "UniRef:UniRef100_Q9PQM5", "UniRef:UniRef50_Q9PQM5", "UniRef:UniRef90_Q9PQM5" ], "nt": "ATGGCAAAATTTATTAAATCTGCACACTATTTTGACCAATATCCAATTGATAAGCAGTTTGAAATATGTGTAATTGGTAGATCAAATGTCGGTAAGTCAAGTTTAATTAATGCATTAGCAAATAAAAAAATTGCAAGGACTTCAAATACGCCTGGAAGAACACAGTTAGTAAATTTTTTTGATTTCAATAATTTTAGATTGGTTGATTTACCGGGATATGGGTTTGCAAAAGTTAGTAAAGAAAAACAAACGGATTTAGCAGCCATTATTGATCAATATTTAGGGTATCGCCAAAACTTGTGTGCTGTTTTTCAAATTTGCGATATTAACGTATTAACAAATGATGATGTAGAAATGAGTCGATATTTTGAGAATCAAAAATACGCTCATTTTGTTGTTTTAAATAAATTAGATAAAGTTAATAAATCATATTTTAATAATAACAAGCATAAAATAGCTAAATTTTTAAATATTAGCACCGATCGTTTATTATGTGTTTCTGCACAAAATAATATAAATATTATCACATTATTTGCGTTAATGAAAAAAGTTGTAATCCAAGCAAAACAAGAAAAAATACTTTCAAAAAAAGAGGAGAAGATGAGTAGTGAAGAAGAAATTAAGTAA", "aa": "MAKFIKSAHYFDQYPIDKQFEICVIGRSNVGKSSLINALANKKIARTSNTPGRTQLVNFFDFNNFRLVDLPGYGFAKVSKEKQTDLAAIIDQYLGYRQNLCAVFQICDINVLTNDDVEMSRYFENQKYAHFVVLNKLDKVNKSYFNNNKHKIAKFLNISTDRLLCVSAQNNINIITLFALMKKVVIQAKQEKILSKKEEKMSSEEEIK", "aa_hexdigest": "8c0818ecb1b5504ffdff4646aa8646e5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129F33", "ncbi_nrp_id": "WP_006688825.1", "uniref100_id": "UniRef100_Q9PQM5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129F33", "RefSeq:WP_006688825.1", "UniRef:UniRef100_Q9PQM5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQM5", "uniref90_id": "UniRef90_Q9PQM5" }, "psc": { "uniref90_id": "UniRef90_Q9PQM5", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_Q9PQM5", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQM5" ], "product": "Probable GTP-binding protein EngB" }, "genes": [ "yihA" ], "id": "GPKLONFDEH_541", "locus": "GCA000006625_00508" }, { "type": "cds", "sequence": "contig_1", "start": 301779, "stop": 302015, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGCCCCCTCCTAATGTTACAGGTCATTTACATATTGGACATGCCTATGATTTTACCTTATCTGATATTTTAATGCGTTATAAGAAACTAAAAGGATATGATTCCTTTATTATTCCTGGGACAGACCATGCAGGGATCGCAACACAGACAAAATTTGAAAAAAATTTAAAAGTAAACGCACAAACCAATCGTTTCAATTTAGGGCGAGAACTGTTTTTAGAAAAGTTAAAAATTTGA", "aa": "MPPPNVTGHLHIGHAYDFTLSDILMRYKKLKGYDSFIIPGTDHAGIATQTKFEKNLKVNAQTNRFNLGRELFLEKLKI", "aa_hexdigest": "4bcffa11b0e9900daae018cc700a0615", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 76, "aa_cov": 0.9743589743589743, "hmm_cov": 1.0, "evalue": 9.4344646457579e-25, "score": 82.73184204101562, "start": 2, "stop": 77 } ], "seq_stats": { "molecular_weight": 8914.235799999999, "isoelectric_point": 9.572539710998534 }, "id": "GPKLONFDEH_542", "locus": "GCA000006625_00509" }, { "type": "cds", "sequence": "contig_1", "start": 302064, "stop": 302189, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGGTTTAGATTATAGTAATTATTTATTTACATTAGATCCAATAGTTGTTCAAACAGTGCGCGAAGTTTTTGTAAAAATGTTTAATGAACATATTATTTATCGTGATAAAAAATTAGTTAATTGA", "aa": "MGLDYSNYLFTLDPIVVQTVREVFVKMFNEHIIYRDKKLVN", "aa_hexdigest": "d0df84e6b47b0b2d506ec1081f066941", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 39, "aa_cov": 0.9512195121951219, "hmm_cov": 1.0, "evalue": 5.293273180215144e-08, "score": 27.561874389648438, "start": 3, "stop": 41 } ], "seq_stats": { "molecular_weight": 4949.7439, "isoelectric_point": 6.521330833435059 }, "id": "GPKLONFDEH_543", "locus": "GCA000006625_00510" }, { "type": "cds", "sequence": "contig_1", "start": 302226, "stop": 302738, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "GTGATTCATAAAGAAGTTGAACAAAAACTGTATTATATAAAATATTTAAGTCAAGATCAAAAGGATTTTGTTGTTGTAGCCACTTCACGCCCTGAAACTATGTTTGGTGATAAATATTTAGTTATAAATCCTAAAGATAAACGTTATTTTCATTTACATAATAAAATTTTTATTAATCCAATTAATAATATAGAAATGACTGTTATTTTGGATGATTATATTGATATTGAATTTGGAACAGGAGTGATGAAGTGTACGCCTGCTCACGATTTTAATGACTATGAATTAGCTAAAAAACATAATTTAGAAATTATTAATATTATGAACGCTGATGGTACTTTAAATGAAAAATGTGGGGAATTTAAGGGTTTAGATCGATTAGAAGCTCGTTCTTTAATAATTGATAAATTACAGAAAAATAATCATTTATTAAAAGTAGAATCTTATCGAACCAGCGTTGGTTTTAGTGAGAGAACTAATGAAATTGTTGAACCTTATTTATCACATCAATGA", "aa": "MIHKEVEQKLYYIKYLSQDQKDFVVVATSRPETMFGDKYLVINPKDKRYFHLHNKIFINPINNIEMTVILDDYIDIEFGTGVMKCTPAHDFNDYELAKKHNLEIINIMNADGTLNEKCGEFKGLDRLEARSLIIDKLQKNNHLLKVESYRTSVGFSERTNEIVEPYLSHQ", "aa_hexdigest": "70c3de9c8801e180e40ee0f35d9bde04", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 58, "aa_cov": 0.3411764705882353, "hmm_cov": 1.0, "evalue": 6.374864115667002e-16, "score": 53.96089172363281, "start": 55, "stop": 112 } ], "seq_stats": { "molecular_weight": 19944.593500000006, "isoelectric_point": 5.971356773376464 }, "id": "GPKLONFDEH_544", "locus": "GCA000006625_00511" }, { "type": "cds", "sequence": "contig_1", "start": 302748, "stop": 302846, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGTTTAATAAAAGATACAATCAAAATGCAAGATGATTGTAATAATAAAGTAGATTTTTATCCAAATCGTTTTAATAAGACTTTACTTACATGA", "aa": "MDSLIKDTIKMQDDCNNKVDFYPNRFNKTLLT", "aa_hexdigest": "124a7c2cc837858b6b98ce7b7a55a9a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3822.3457000000008, "isoelectric_point": 5.897920799255371 }, "id": "GPKLONFDEH_545", "locus": "GCA000006625_00512" }, { "type": "cds", "sequence": "contig_1", "start": 303132, "stop": 303365, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGATGAACTTTAGCCAATATTTGGTTGAAAAAAAACCTTTTAAGGATGTTTTAATTCATGGTTTAATTCGTGATTCACAGGGACGTAAGATGTCAAAATCATTAGGGAATGGTATTGATCCTTTTGACATTATTGATAAATATGGATTAGATGCTATGCGTTTATTTTTTGCATCATGTACAACAATTGGTGAAGATTTAAATTTTTCAACAGAACGATTAGGCGCTAATTGA", "aa": "MMNFSQYLVEKKPFKDVLIHGLIRDSQGRKMSKSLGNGIDPFDIIDKYGLDAMRLFFASCTTIGEDLNFSTERLGAN", "aa_hexdigest": "921e8c30a9758ee52e332fdb0d7f8928", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001D01CCB6", "query_cov": 0.948051948051948, "subject_cov": 0.8390804597701149, "identity": 0.562, "score": 89.4, "evalue": 8.49e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PEY5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 68, "aa_cov": 0.8831168831168831, "hmm_cov": 0.9852941176470589, "evalue": 1.1706537838303808e-24, "score": 82.42316436767578, "start": 3, "stop": 70 } ], "seq_stats": { "molecular_weight": 8706.936999999994, "isoelectric_point": 6.540030860900879 }, "id": "GPKLONFDEH_546", "locus": "GCA000006625_00513" }, { "type": "cds", "sequence": "contig_1", "start": 303945, "stop": 304277, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10458.14" ], "nt": "TTGTTATTAATCTTAGTTAATGCTGAAATAAATGAAATTAAAAAAACAAGTTCTAATAATTATACTTATGAGCTAGTTATTGATGATTTTATTCTTAAAACTTCGTATGAAAAACCGATTGATTATGTTTTTCAAATGAAAAAAGCTAGTGAACAGTTAAACTATTTGGAAAACGAAATTCAACGAGCAACTAATTTATTAAATAATTCAGGTTTTATCAACAAAGCCCCTGCTCAATTAATAATTAAAGAAAAAAATAAATTAATTAATTTAAAAAAAGAACACGCAAATTTATTAAAAACTCTTACTGACTTAAAACAAAAGGTGAAATAA", "aa": "MLLILVNAEINEIKKTSSNNYTYELVIDDFILKTSYEKPIDYVFQMKKASEQLNYLENEIQRATNLLNNSGFINKAPAQLIIKEKNKLINLKKEHANLLKTLTDLKQKVK", "aa_hexdigest": "472c58c25469d68599b1eb645e7cf879", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10458.14", "name": "Val_tRNA-synt_C", "length": 57, "aa_cov": 0.5181818181818182, "hmm_cov": 1.0, "evalue": 3.643999284582947e-11, "score": 38.809513092041016, "start": 50, "stop": 106 } ], "seq_stats": { "molecular_weight": 12799.770099999976, "isoelectric_point": 9.222669792175292 }, "id": "GPKLONFDEH_547", "locus": "GCA000006625_00514" }, { "type": "cds", "sequence": "contig_1", "start": 304282, "stop": 304800, "strand": "+", "frame": 1, "gene": "trmL", "product": "Putative tRNA (cytidine(34)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0219", "COG:J", "EC:2.1.1.207", "KEGG:K03216", "RefSeq:WP_006688833.1", "SO:0001217", "UniParc:UPI00000C1BF4", "UniRef:UniRef100_Q9PQM3", "UniRef:UniRef50_Q9PQM3", "UniRef:UniRef90_Q9PQM3" ], "nt": "ATGTTAAATATTGTTTTATTTGAACCAGAAATTATTTTAAACACTGGTAATATCGCTCGTATGTGTGTAGGTTTTGATGCAAATTTACATCTTGTTAGACCATATGGATTTATTTTAGATAAAAAACGTTTTCACAAAGATTTTATACGTGCCTCAACCAATCATTTAAATGCCTTAAAATTATATGAATATGATGATTTTAATGAATTTATTAATAAAAACAACATTAGTGGTGATGAAATATATTTTTTTACTAGATATGGTACTAAAACTCCGTGCGATTATCAATATCAAAATATAATTCAACATAATGTATATTTAGTTTTTGGTAAAGAATCAACAGGGATAGATCATAATATTTTAAAAAAATATCCTCATAATTGGATCCGTATTCCTACAAGCATTAATTTGAGAAGTTTAAATATTGCTAATAGTGTTGCTATGGGAATCTATGAAGTAGTGCGGCAAAATAATTTTAAAGATTTATTCAAATATGAACCTCATAAGAAATTTGAGTAA", "aa": "MLNIVLFEPEIILNTGNIARMCVGFDANLHLVRPYGFILDKKRFHKDFIRASTNHLNALKLYEYDDFNEFINKNNISGDEIYFFTRYGTKTPCDYQYQNIIQHNVYLVFGKESTGIDHNILKKYPHNWIRIPTSINLRSLNIANSVAMGIYEVVRQNNFKDLFKYEPHKKFE", "aa_hexdigest": "6daa8fb35ba1cc8e23aa0d1092411841", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1BF4", "ncbi_nrp_id": "WP_006688833.1", "uniref100_id": "UniRef100_Q9PQM3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BF4", "RefSeq:WP_006688833.1", "UniRef:UniRef100_Q9PQM3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQM3", "uniref90_id": "UniRef90_Q9PQM3" }, "psc": { "uniref90_id": "UniRef90_Q9PQM3", "gene": "TrmL", "product": "Putative tRNA (cytidine(34)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_Q9PQM3", "cog_id": "COG0219", "cog_category": "J", "kegg_orthology_id": "K03216" }, "pscc": { "uniref50_id": "UniRef50_Q9PQM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQM3" ], "product": "Putative tRNA (cytidine(34)-2'-O)-methyltransferase" }, "genes": [ "trmL" ], "id": "GPKLONFDEH_548", "locus": "GCA000006625_00515" }, { "type": "cds", "sequence": "contig_1", "start": 304806, "stop": 305168, "strand": "+", "frame": 3, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_Q9PQM2", "UniRef:UniRef90_Q9PQM2" ], "nt": "ATGCGTTTACGAAATAATGCCAATGCTCCACTTTATTTAAAATCACAACACGAATATATAATTAATGATCCACACCTTTTAAAAGATAATTTAGGAAAAATTTTTAAAAATCCTGAATTGCCTCTTTATATTGAAATTGGAATGGGTAAGGGTGATTTTATTATTGAAAATGCCTTACGAAACCAACAAATTAATTATTTAGGTATTGAAAAATTTCCAACAGTAATTGTTAAAGCTCACAAAAAAGCTTTAAAACATAAATTAGATAATTTAGCAATGATTTGTTTTGACGCAAATAAAATTTTAGAATTATTAAATTCTGAAAGTGTAGATAAAATTTATTTAAATTTTTCTGACCCCTGA", "aa": "MRLRNNANAPLYLKSQHEYIINDPHLLKDNLGKIFKNPELPLYIEIGMGKGDFIIENALRNQQINYLGIEKFPTVIVKAHKKALKHKLDNLAMICFDANKILELLNSESVDKIYLNFSDP", "aa_hexdigest": "45c5d220d6fba143948f177ddc8cadd4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 304806, "stop": 305471, "observations": { "mutation": [ 305166 ], "stop": [ 305166 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQM2", "query_cov": 1.0, "subject_cov": 0.5357142857142857, "identity": 1.0, "score": 245.0, "evalue": 7.79e-81, "gene_start": 1, "gene_end": 120, "reference_start": 1, "reference_end": 120, "reference_sequence": "MRLRNNANAPLYLKSQHEYIINDPHLLKDNLGKIFKNPELPLYIEIGMGKGDFIIENALRNQQINYLGIEKFPTVIVKAHKKALKHKLDNLAMICFDANKILELLNSESVDKIYLNFSDPWPKKRHAKKRLTHPCFLEKFAVILKQNALVEFKTDNENLFMYTIYDVLLKDLTKYEILFLTYNLYTLVNNVELLKNIPTEYEKKFVMQGERIKKVNFRFLKNNQ" }, "paralog": false, "identity": 0.995475113122172, "score": 442.0, "evalue": 3.05e-161, "description": "Internal stop codon at 305166. Nonsense mutation around 305166.", "psc": { "uniref90_id": "UniRef90_Q9PQM2", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_Q9PQM2", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQM2", "EC:2.1.1.33", "UniRef:UniRef50_Q9PQM2", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQM2" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "GPKLONFDEH_549", "locus": "GCA000006625_00516" }, { "type": "cds", "sequence": "contig_1", "start": 305286, "stop": 305480, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACTATTTATGATGTATTGCTAAAGGATTTAACAAAGTATGAAATTTTATTTTTAACTTATAATTTATACACACTTGTTAACAATGTTGAATTATTAAAAAATATTCCTACCGAATATGAAAAAAAATTTGTTATGCAGGGCGAGCGTATTAAAAAAGTCAATTTTCGTTTTCTTAAAAATAATCAATAA", "aa": "MYTIYDVLLKDLTKYEILFLTYNLYTLVNNVELLKNIPTEYEKKFVMQGERIKKVNFRFLKNNQ", "aa_hexdigest": "4f31d40bca77bdad11431a9c7f8e3d4b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7856.160299999999, "isoelectric_point": 9.222540855407715 }, "id": "GPKLONFDEH_550", "locus": "GCA000006625_00517" }, { "type": "tRNA", "sequence": "contig_1", "start": 305518, "stop": 305596, "strand": "+", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 67.2, "nt": "GCGGTTATGGTGAAGTTGGTTAACACGCCTGATTGTGGCTCAGGTATTTTCGCGGGTTCGAGTCCCGTTAATCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 305527, 305529 ], "id": "GPKLONFDEH_4", "locus": "GCA000006625_00518" }, { "type": "cds", "sequence": "contig_1", "start": 305728, "stop": 305946, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAATAAAAACAAAAAATTTCATTTTCAGTATTTTAAAAAAGATAACGATCAATCAATTTCTACTAAAGAAACATTAAAAAGATATTTTAATTTTAGGTCTCAAAGTGTAAAATTGATTATTTTTAATTTAGTTTTAACAGCATTTTTTCTTGCATTATTTTTAATTGGTCGTTTTTTAACTAAAAATTTAGATTTTTTAAATGGTTTTAGTTGA", "aa": "MINKNKKFHFQYFKKDNDQSISTKETLKRYFNFRSQSVKLIIFNLVLTAFFLALFLIGRFLTKNLDFLNGFS", "aa_hexdigest": "4ca776154467a1a7dd255c7a6b5ce919", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8650.058899999989, "isoelectric_point": 10.234565544128415 }, "id": "GPKLONFDEH_551", "locus": "GCA000006625_00519" }, { "type": "cds", "sequence": "contig_1", "start": 305956, "stop": 306228, "strand": "+", "frame": 1, "gene": null, "product": "ECF transporter S component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0E1", "UniRef:UniRef90_A0AAC9T0E1" ], "nt": "ATGGGAATTTTTGTTTTAGCAATCGTTTGTATTCCCAATTTTTATTATAAATTAATGTATTATATTATTGCGCCGTTAGTGATGTTAGCAATTGGTTTTAGTGCTGAGCCCTTCTTTGGATATTTAATGCCACATTATGGTTTTGGTTTAATTTTATTTACGGATGTCATTTTAATTATTGTTAAAAAGTCATGTCAGTCAATTAAAAAGCAAATATTTTTATCCTATTTATTAATTTTTAGCTTTTCAATCATTGGTTATTTTATTGTTTGA", "aa": "MGIFVLAIVCIPNFYYKLMYYIIAPLVMLAIGFSAEPFFGYLMPHYGFGLILFTDVILIIVKKSCQSIKKQIFLSYLLIFSFSIIGYFIV", "aa_hexdigest": "cf9c511021059bb1fe1b46dafbe7286d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 305728, "stop": 306399, "observations": { "mutation": [ 305944, 306226 ], "stop": [ 305944, 306226 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0E1", "query_cov": 1.0, "subject_cov": 0.40358744394618834, "identity": 1.0, "score": 168.0, "evalue": 3.81e-51, "gene_start": 1, "gene_end": 90, "reference_start": 77, "reference_end": 166, "reference_sequence": "MINKNKKFHFQYFKKDNDQSISTKETLKRYFNFRSQSVKLIIFNLVLTAFFLALFLIGRFLTKNLDFLNGFSWQLQMGIFVLAIVCIPNFYYKLMYYIIAPLVMLAIGFSAEPFFGYLMPHYGFGLILFTDVILIIVKKSCQSIKKQIFLSYLLIFSFSIIGYFIVWMGYSIQGTLFYNTAFIPSMIYNSLVTFGSMAINFVLYIISIPAIVALKNKYLCKLV" }, "paralog": false, "identity": 0.9910313901345291, "score": 434.0, "evalue": 2.05e-158, "description": "Internal stop codon at 305944, 306226. Nonsense mutation around 305944, 306226.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0E1", "product": "ECF transporter S component", "uniref50_id": "UniRef50_A0AAC9T0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0E1", "UniRef:UniRef50_A0AAC9T0E1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0E1" ], "product": "ECF transporter S component" } }, "id": "GPKLONFDEH_552", "locus": "GCA000006625_00520" }, { "type": "cds", "sequence": "contig_1", "start": 306229, "stop": 306399, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCTATAGTATTCAAGGAACACTTTTTTACAATACTGCTTTTATTCCTTCTATGATTTATAATTCTTTGGTAACTTTTGGTTCAATGGCAATTAATTTTGTATTATATATAATTTCAATTCCCGCTATTGTTGCCTTAAAAAACAAATATTTATGTAAATTAGTTTAA", "aa": "MGYSIQGTLFYNTAFIPSMIYNSLVTFGSMAINFVLYIISIPAIVALKNKYLCKLV", "aa_hexdigest": "acda50cfe7ed6e0e849d23752029badc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6257.488399999998, "isoelectric_point": 9.302675056457517 }, "id": "GPKLONFDEH_553", "locus": "GCA000006625_00521" }, { "type": "cds", "sequence": "contig_1", "start": 306702, "stop": 307154, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAAGATTTACAAAACGAACCTGAAATTTTAGATGTTATTAAAAACAATCCACAGATCTTAAAAACACTTAATGATAACCGCGAATTTTTAAAACAAATTGTTGAATATAAGCCATTTGCTGAAATTCTTAGCAAGCAACCTGAAATTTTAAAATTTGTTCAAAATAATAAAGAATTAATTAAACAGATACAAAATTTACCTGAATTATTAGATTTAATTAAAAAACACCAAAATTTAATTGATCACTTAAAAACTAACGAAAAATATATAGAAGTTATTAAAGCTAATCCTCAAATGTTAAAAGATCTCTATCATGTAGATGATAATTTATTTAATATTATAAAGACAAGCTTAGAAAATGATAAAGAAAATAATATTTTAATGTTGGCTATTAAATATTCAGTATTAATTAATAATTTTACTAAGAATTTAACTTTACAAGAATGA", "aa": "MIKDLQNEPEILDVIKNNPQILKTLNDNREFLKQIVEYKPFAEILSKQPEILKFVQNNKELIKQIQNLPELLDLIKKHQNLIDHLKTNEKYIEVIKANPQMLKDLYHVDDNLFNIIKTSLENDKENNILMLAIKYSVLINNFTKNLTLQE", "aa_hexdigest": "38badd23233fa10c15d62275f59d5b53", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17763.476300000002, "isoelectric_point": 5.961921501159668 }, "id": "GPKLONFDEH_554", "locus": "GCA000006625_00522" }, { "type": "cds", "sequence": "contig_1", "start": 307215, "stop": 307820, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3I0", "UniRef:UniRef90_A0AAC9T3I0" ], "nt": "TTGTTTAATATTATTAAAAATAATAATCAATTATTTGAAGTTTTTTTAAATAATTATGACTACATAAAAAATCTTGAGGACAATCAAAAAATTATTGATATTATTCAACAAAATCCTGATTTAATTGATGATTTTAAAAAGATTGATTTTGATCTTTTAACACAAAAAAGGAATTTTGTTCGTGCACTAATTAAAATAATTGATTATAAACAACCCAAAGAAATTCAATTCCTTAACAAAAAAGTTTTTTTAGATTTCTTTAAAAATAAATCTGAAATTACCAATGCTTTAAACATCACATCTTTAATTGGATTTGTATTGTTTATAGTAATTTATACAATTTTTATTTTTGCATCTTTTATTTCTCATATTCTAATGTTAAAACAAATTAAAGATTTTAATAAAATTTTTAAAGAAGCTAAATTAAAAAAAATATATCTTATAAGCACTTTATTATTACAATTTTTATTTTTAATTTTATTCATTATTAATTTTATAGTATTAATTATTCTAGTTGTAGATTATAATAAATTGAAAGCAATAGTTTTAAATAGTGGTATAAAAAGAAAAGTGGAGGATAGTGATGCAAGATTGCATTTTTTGTAA", "aa": "MFNIIKNNNQLFEVFLNNYDYIKNLEDNQKIIDIIQQNPDLIDDFKKIDFDLLTQKRNFVRALIKIIDYKQPKEIQFLNKKVFLDFFKNKSEITNALNITSLIGFVLFIVIYTIFIFASFISHILMLKQIKDFNKIFKEAKLKKIYLISTLLLQFLFLILFIINFIVLIILVVDYNKLKAIVLNSGIKRKVEDSDARLHFL", "aa_hexdigest": "dcd7b56f2292afc571a600c5bf0c1e00", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 306996, "stop": 307820, "observations": { "mutation": [ 307152 ], "stop": [ 307152 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3I0", "query_cov": 1.0, "subject_cov": 0.43982494529540483, "identity": 0.905, "score": 276.0, "evalue": 2e-88, "gene_start": 1, "gene_end": 201, "reference_start": 257, "reference_end": 457, "reference_sequence": "MFMFDQIFKKRKRHLNFQIFIFFFVLILITFLILWIISGNVQTIIDFIKKKIYDASPQLKYLTNIQIKPFKNLEEIFDFIIKNKNLIKDLQNEPEILDVIKNNPQILKTLNDNREFLKQIVEYKPFAEILSKQPEILKFVQNNKELIKQIQNLPELLDLIKKHQNLIDHLKTNEKYIEVIKANPQMLKDLYHVDDNLFNIIKTSLENDKENNILMLAIKYSVLINNFTKNLTLQEWKQIIEFYKTVDLNKFIVDDNLFNIIKNNNQLFEVFLNNYDYIKNLEDNQKIIDIIQQNPDLIDDFKKIDFDLLTQKRNFVRALIKIIDYKQPKEIQFLNKKVFLDFFKNKSEITNALNITSLIGFVLFIVIYTIFIFASFISHILMLKQIKDFNKIFKEAKLKKIYLISTLLLQFLFLILFIINFIVLIILVVDYNKLKAIVLNSGIKRKVEDSDARLHFL" }, "paralog": false, "identity": 0.9963503649635036, "score": 428.0, "evalue": 1.37e-150, "description": "Internal stop codon at 307152. Nonsense mutation around 307152.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3I0", "product": "ABC transporter permease", "uniref50_id": "UniRef50_A0AAC9T3I0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3I0", "UniRef:UniRef50_A0AAC9T3I0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T3I0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3I0" ], "product": "ABC transporter permease" } }, "id": "GPKLONFDEH_555", "locus": "GCA000006625_00523" }, { "type": "cds", "sequence": "contig_1", "start": 307798, "stop": 308256, "strand": "+", "frame": 1, "gene": "hinT", "product": "HIT family protein", "db_xrefs": [ "COG:COG0537", "COG:FR", "RefSeq:WP_006688907.1", "SO:0001217", "UniParc:UPI00000C1BF7", "UniRef:UniRef100_A0AAC9T389", "UniRef:UniRef50_A0A084F0Z8", "UniRef:UniRef90_A0AAC9T389" ], "nt": "ATGCAAGATTGCATTTTTTGTAAAATTTTAAAAGGTGAAATTATTTCAAAAATAATAGATGAAAACGAATTTGCTATAGCAATTTTAGACATTCAACCTGCAAGTGATGGTCATATGTTGATTATTCCTAAAAAACATTATCGCAATTTTTCACTAACTGATCCTATTTATCTAGATGGTATGATGCGTTTAGCAAAAAATATGACATTTGTTTTAGAAGAAGTTTTTCCTAATGTTTTAGGTTTTAATTATTTAATGAATTCAAATTCTAGTGCTGGTCAAGTAATCATGCACACACATTTACACATCATTCCAAAACAAAGTAATGGACGTGGTTTTGTTTTTAAAGCAATCAAAGAAGAAGGCGATATTAGTAATATTGATGAAATTTATAAAAAAATCATTATTAAAACACAAAAATTAAAAAAATCACGTTTAAGTAAGCATTATATGGTTTAA", "aa": "MQDCIFCKILKGEIISKIIDENEFAIAILDIQPASDGHMLIIPKKHYRNFSLTDPIYLDGMMRLAKNMTFVLEEVFPNVLGFNYLMNSNSSAGQVIMHTHLHIIPKQSNGRGFVFKAIKEEGDISNIDEIYKKIIIKTQKLKKSRLSKHYMV", "aa_hexdigest": "2ba2918b8647ee272448115095505151", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1BF7", "ncbi_nrp_id": "WP_006688907.1", "uniref100_id": "UniRef100_A0AAC9T389", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BF7", "RefSeq:WP_006688907.1", "UniRef:UniRef100_A0AAC9T389" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T389", "uniref90_id": "UniRef90_A0AAC9T389" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T389", "gene": "HinT", "product": "HIT family protein", "uniref50_id": "UniRef50_A0A084F0Z8", "cog_id": "COG0537", "cog_category": "FR" }, "pscc": { "uniref50_id": "UniRef50_A0A084F0Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F0Z8" ], "product": "Histidine triad protein" }, "genes": [ "hinT" ], "id": "GPKLONFDEH_556", "locus": "GCA000006625_00524" }, { "type": "cds", "sequence": "contig_1", "start": 308419, "stop": 308688, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAAAAGTTTTTTTAAACAAAAACCGAGATATTATTTCTAAAATTCCATTAAATCAAGTTAAAAAAATAGCTAATGACATTTTAGAAAAAGAAATTATTTTAGAACAGCCTTTAGTTGATTATTATTACCAAAGTTCATCTTGTTTTATTTTAATACCTTATTTGATTCAAATTCTTTATCAATCTTATCACCTTAATAAACCAATTTATAAAATAATGTGTAAATTTATTATTAGAAATAATTTAGCATTATTTAAAGAATGA", "aa": "MILKVFLNKNRDIISKIPLNQVKKIANDILEKEIILEQPLVDYYYQSSSCFILIPYLIQILYQSYHLNKPIYKIMCKFIIRNNLALFKE", "aa_hexdigest": "37a43775bc428f98a35f719be37ba477", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10681.725699999988, "isoelectric_point": 9.275147056579591 }, "id": "GPKLONFDEH_557", "locus": "GCA000006625_00525" }, { "type": "cds", "sequence": "contig_1", "start": 309018, "stop": 309185, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGTTTAAGGTTAAAAGATGGATTAAATCTAAATAAAAAAATTAATTATGAAGCTTATTCATATTTTAAAAATCAATTGAAACACGTTAGTATTAATAAAAAAAATCATCTTCTTATTGATGATTTAAATTTACTAAATTTAACTATTTTAGATATATTTTAA", "aa": "MMGLRLKDGLNLNKKINYEAYSYFKNQLKHVSINKKNHLLIDDLNLLNLTILDIF", "aa_hexdigest": "0a4d9074a8adfcbcf2c2b3e9074ab8de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6505.607699999999, "isoelectric_point": 9.398539543151855 }, "id": "GPKLONFDEH_558", "locus": "GCA000006625_00526" }, { "type": "cds", "sequence": "contig_1", "start": 309372, "stop": 310082, "strand": "-", "frame": 2, "gene": null, "product": "radical SAM protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3EC27E", "UniRef:UniRef90_UPI001E3EC27E" ], "nt": "ATGATTTTTAACAAAAAAACTAAACATTTATACATTCATATTCCATTTTGTAACTATATTTGTATATTTTCTGATTTTAAACGTATTTTAAAAACTCCTCAAACAAAAAAATTTTTTAAGAATTTTTTAGAAAATATTAAAATGCACATTAATAATTTTAAAATAAAGCAATTTAAAACAATCTACTTAGGCGGAGGTGTTCCAAATTGTTTAAACAATCAAGAATTAGAAATTTTATTAAAAACAATTTCTCCTTATGTTGATGATAATTGCGAATATACCATTGAATGTAATCCTGAATTAATAAATCAAACTCAAATTAATCTCTTCAAAAAATACAAAATTAATCGCATTAGTCTTAACGTTCAATCAATGAATAATGATGTGTTAAAACAAATGAATTTTATTCATACAAATCAAGATAGTAAAAAAGCGATTAATTTATTACATAAAAACGGATTGTATAATGTGTCTTGTGATTTTTTATTTTGCATATCAATCTTAACATTAAAAGACTTAGATGATGTTTTTAATTTTATTTTAAATCATAAAATTAATCATGTTAGTTTTTATAGTTTAGAAATTAAAGAACAGTCACTTTTAAAAAAGTACCATATTACTATCGATGAAGAAAAAGAAATTGAACAAATGAATTATATAATTAATAAATTTGCAGGATTAAATTTTCATCGTTATGAAGTATGTAATTGA", "aa": "MIFNKKTKHLYIHIPFCNYICIFSDFKRILKTPQTKKFFKNFLENIKMHINNFKIKQFKTIYLGGGVPNCLNNQELEILLKTISPYVDDNCEYTIECNPELINQTQINLFKKYKINRISLNVQSMNNDVLKQMNFIHTNQDSKKAINLLHKNGLYNVSCDFLFCISILTLKDLDDVFNFILNHKINHVSFYSLEIKEQSLLKKYHITIDEEKEIEQMNYIINKFAGLNFHRYEVCN", "aa_hexdigest": "305bce1da4f757d0138eb449cb82d253", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E3EC27E", "query_cov": 1.0, "subject_cov": 0.8489208633093526, "identity": 1.0, "score": 482.0, "evalue": 1.22e-171, "valid": true, "product": "radical SAM protein", "uniref50_id": "UniRef50_UPI001E3EC27E" }, "pscc": { "uniref50_id": "UniRef50_UPI001E3EC27E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3EC27E" ], "product": "radical SAM protein" }, "genes": [], "id": "GPKLONFDEH_559", "locus": "GCA000006625_00527" }, { "type": "cds", "sequence": "contig_1", "start": 310097, "stop": 310309, "strand": "-", "frame": 3, "gene": null, "product": "DUF4044 domain-containing protein", "db_xrefs": [ "RefSeq:WP_006688920.1", "SO:0001217", "UniParc:UPI000165F8A8", "UniRef:UniRef100_A0AAC9T0W5", "UniRef:UniRef50_A0AAC9T0W5", "UniRef:UniRef90_A0AAC9T0W5" ], "nt": "ATGAGTACAAAAGCATTTAACCCAAAAGACCGTGTTACCAAAACTGTCGTTTTAAGCGATTTTGATCAGCATCAAATTTATTCAAAAAAACATAAAAAAATGGACTTTAAAAAACGCGTTATTTTATCGATTTTTCTAATAATATTAACTTGCGGAGCAATTGTTTTATTAGTTTTTGTTATTACTGAATTATTTAGATTATTAAGTGCATAA", "aa": "MSTKAFNPKDRVTKTVVLSDFDQHQIYSKKHKKMDFKKRVILSIFLIILTCGAIVLLVFVITELFRLLSA", "aa_hexdigest": "01334c390fd057ec20ee58c95c7257e7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI000165F8A8", "ncbi_nrp_id": "WP_006688920.1", "uniref100_id": "UniRef100_A0AAC9T0W5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8A8", "RefSeq:WP_006688920.1", "UniRef:UniRef100_A0AAC9T0W5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0W5", "uniref90_id": "UniRef90_A0AAC9T0W5" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0W5", "product": "DUF4044 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0W5" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0W5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0W5" ], "product": "DUF4044 domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_560", "locus": "GCA000006625_00528" }, { "type": "cds", "sequence": "contig_1", "start": 310465, "stop": 310704, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGGATTTAAAAGTTAGAATTATGGGATTAAAAAAAGAAAAAAAAGATCGTTCAACGATTCAATTATTATTTGGAACAATTAAAAAACGCAAAATTGCGAACTATTTAATTATTGCTTTTGCCTTTTTAACATTTGCCTTAGCAATTATAGGAAGTTTTGAAGCTATTGCATCAAAATATGCTATTGGCATTGGATCAGGGCTTTCATTATTTATTACGATTGCTTTTAGTGTTTGA", "aa": "MEDLKVRIMGLKKEKKDRSTIQLLFGTIKKRKIANYLIIAFAFLTFALAIIGSFEAIASKYAIGIGSGLSLFITIAFSV", "aa_hexdigest": "847ed7c86a561211980a86e4f7246acd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8715.444199999994, "isoelectric_point": 10.073716926574708 }, "id": "GPKLONFDEH_561", "locus": "GCA000006625_00529" }, { "type": "cds", "sequence": "contig_1", "start": 311037, "stop": 311366, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02749.22" ], "nt": "ATGGGACCAATTATTCCAAATACACGTTTAATTGATTTTAAATTTGATCGAGACTTATTAAATAGTGCCTATACTGCTCATTATTTTATTAAAACATGCAAGATAATTGAATTGCATGCACCAAATCATTCAGTTATTATGCAATTTACACACTTTAGCAAAACACCGATCATGGTGTGTGGAACAAGTGAGGTTTTAGCATTATTAGAGTTTTGTTTATCTAAAAAAGAACTAAAACAATTAAAAATATATTATGTTCCAGATGGACATGTTATTAAACCAAAAGAAGCTTTATTTGTTATCGAAGGGCCATATGAAATTTTTGGCTGA", "aa": "MGPIIPNTRLIDFKFDRDLLNSAYTAHYFIKTCKIIELHAPNHSVIMQFTHFSKTPIMVCGTSEVLALLEFCLSKKELKQLKIYYVPDGHVIKPKEALFVIEGPYEIFG", "aa_hexdigest": "65b04fae2e61639a71f11b0188830785", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02749.22", "name": "QRPTase_N", "length": 67, "aa_cov": 0.6146788990825688, "hmm_cov": 0.8507462686567164, "evalue": 9.81504636574734e-10, "score": 34.18281555175781, "start": 42, "stop": 108 } ], "seq_stats": { "molecular_weight": 12516.666199999989, "isoelectric_point": 7.745301628112793 }, "id": "GPKLONFDEH_562", "locus": "GCA000006625_00530" }, { "type": "cds", "sequence": "contig_1", "start": 311469, "stop": 312128, "strand": "+", "frame": 3, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:6.3.4.21", "KEGG:K00763", "SO:0001217", "UniRef:UniRef50_Q9PQL5", "UniRef:UniRef90_Q9PQL5" ], "nt": "ATGAGTGATCGTTCTGATGATTATAGTTTACAACCATATGATGGGTATGCTGCTGCTGTTGGTGGAATGCAATACTTTGTTACGCAAAAGCAGGTTGAATTTTTAAAAGATATTAATTATGAATGTAAGGTAATTGGCTCAATGCCACATGCTTTAATTCAACAAAACAATGGTCGGATTGATTTAGCATGTGAAATGTTTGCACAAACTTTTCCTAGCGATCCTTTAATTGCTGTTATTGATTATAATAACAATGTTTTAAATGATCTTGAACAACTACGATATATGTTTGATCGTTTGTTTGCAGTACGAATTGATACAGCTAAGGATTTAATTGATGAAAGTTTATTATCGACTTTTAATAATGTTGGTAATCGTGATTTACATGGTTGTAATCCTTATCTTATTGCTTTAGTACGTGAATATTTAGATAATAATGGAGGAAAACATATTAAAATAATTGCTTCAAGTGGGATTGATTTAAATGGTATTAAAAATTTTAATAAACACAATTCTGCAATTGATTTTTATGGAATAGGCACATATTTAAGTCACTTATCAGTTCACATCACTGCTGACTTGGTTTGTTTAGATAATGTTTATGGTGCTAAAGTTGGCCGTAAAATCGCTAAGAATTTTTCTGAAATGATTTTATATTAA", "aa": "MSDRSDDYSLQPYDGYAAAVGGMQYFVTQKQVEFLKDINYECKVIGSMPHALIQQNNGRIDLACEMFAQTFPSDPLIAVIDYNNNVLNDLEQLRYMFDRLFAVRIDTAKDLIDESLLSTFNNVGNRDLHGCNPYLIALVREYLDNNGGKHIKIIASSGIDLNGIKNFNKHNSAIDFYGIGTYLSHLSVHITADLVCLDNVYGAKVGRKIAKNFSEMILY", "aa_hexdigest": "981d7aa43626818ed1f8e49c18584514", "start_type": "ATG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "5-prime", "pseudogene": { "start": 311169, "stop": 312128, "observations": { "mutation": [ 311364 ], "stop": [ 311364 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQL5", "query_cov": 1.0, "subject_cov": 0.6033057851239669, "identity": 0.927, "score": 419.0, "evalue": 5.95e-146, "gene_start": 1, "gene_end": 219, "reference_start": 145, "reference_end": 363, "reference_sequence": "MGPVIPNTRLIDFKFDRDLLNKAYTSHYFIKTCKIIELHAPSHSVIMQFTHFSKTPIMVCGTSEVLALLEFCLSRKELKQLKIYYVPDGHVIKPKEALFAIEGPYEIFGWLENIIDSILARRSSVATNCYNVLNVINDEQKVIYMSDRSDDYSLQPYDGYAAAVGGMQYFVTQKQVEFLKDINYECKVMGSMPHALIQQNNGRVDLACEMFAQTFPNDPLIAVIDYNNNVLNDLEQLRYMFDRLYAVRIDTAKDLIDNSLLSTFDNVRNHDLHGCNPYLIDLVREYLDNNGGEHIKIIASSAIDLNSIKNFNKHNSAIDFYGIGTYLTHLSIHITADLVCLDNVYGAKVGRKIAKNFAEMTLY" }, "paralog": false, "identity": 0.9341692789968652, "score": 609.0, "evalue": 2.74e-223, "description": "Internal stop codon at 311364. Nonsense mutation around 311364.", "psc": { "uniref90_id": "UniRef90_Q9PQL5", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "6.3.4.21" ], "uniref50_id": "UniRef50_Q9PQL5", "cog_id": "COG1488", "cog_category": "H", "kegg_orthology_id": "K00763", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQL5", "EC:6.3.4.21", "UniRef:UniRef50_Q9PQL5", "COG:COG1488", "COG:H", "KEGG:K00763" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQL5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQL5" ], "product": "nicotinate phosphoribosyltransferase" } }, "id": "GPKLONFDEH_563", "locus": "GCA000006625_00531" }, { "type": "cds", "sequence": "contig_1", "start": 312204, "stop": 312371, "strand": "+", "frame": 3, "gene": "rpsU", "product": "30S ribosomal protein S21", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_006688933.1", "SO:0001217", "UniParc:UPI0000134E8E", "UniRef:UniRef100_Q9PQL4", "UniRef:UniRef50_Q9PQL4", "UniRef:UniRef90_Q9PQL4" ], "nt": "ATGTCAAGAGGTGTAAGCGTTGAAGGCGATTTAGAAAAGGCTCTTAAGAAATTTAAACGTATTTCTAATGAAACAAAAAAAGATTCTAAACGTCACGAATATTATTTAAGTCCAAGAATTCGTCGTAAAGAAAAAATTAAAGAGGCTAATAAATATCGTAGTTTCTAA", "aa": "MSRGVSVEGDLEKALKKFKRISNETKKDSKRHEYYLSPRIRRKEKIKEANKYRSF", "aa_hexdigest": "cb734cf92ba171b24d152225521f83b5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E8E", "ncbi_nrp_id": "WP_006688933.1", "uniref100_id": "UniRef100_Q9PQL4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E8E", "RefSeq:WP_006688933.1", "UniRef:UniRef100_Q9PQL4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQL4", "uniref90_id": "UniRef90_Q9PQL4" }, "psc": { "uniref90_id": "UniRef90_Q9PQL4", "gene": "rpsU", "product": "30S ribosomal protein S21", "uniref50_id": "UniRef50_Q9PQL4", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQL4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQL4" ], "product": "Small ribosomal subunit protein bS21" }, "genes": [ "rpsU" ], "id": "GPKLONFDEH_564", "locus": "GCA000006625_00532" }, { "type": "cds", "sequence": "contig_1", "start": 312495, "stop": 312971, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGATTACACAAAAAATAAGTGAAGAATTAAATAAGGCTTTGGCGAAAATGGGTATTCATGATACACAGGAGACAAAGATTCTCGTTGATAAAACTAAAAATATTAAATTTGGTGATTTTTATACTAACATAGCAATGATTTTAAGTAAAAAAAATAATAAGAGTTCTTTAGAAATAGCTAAAGAAATAGCTAATAATTTTGAACAAGATTTGTTTTTAGAAGTTAATTTGCAGCCACCTGGATTTTTAAATTTTAAATTAAAAGCAAAAGATCATGAAAATTTATTGAAACAAATTTATTATGAAAAAGATAGATTTGGTCAATTCTCTAAAAAGAATATCACTTATAATATTGAATATGTTTCAGCTAACCCCACAGGTTATTTGCATATTGCTCACGCTGCTAATGCTATTTATGGTGATATTTTAGCTAATTTATTAAAAATTTATGGATATGATGTAGAAACTGAATATTGA", "aa": "MITQKISEELNKALAKMGIHDTQETKILVDKTKNIKFGDFYTNIAMILSKKNNKSSLEIAKEIANNFEQDLFLEVNLQPPGFLNFKLKAKDHENLLKQIYYEKDRFGQFSKKNITYNIEYVSANPTGYLHIAHAANAIYGDILANLLKIYGYDVETEY", "aa_hexdigest": "338b6f4d80b9925d95ad81c17031024b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 84, "aa_cov": 0.5253164556962026, "hmm_cov": 1.0, "evalue": 2.6859032959533637e-22, "score": 74.84034729003906, "start": 5, "stop": 87 } ], "seq_stats": { "molecular_weight": 18203.625399999997, "isoelectric_point": 6.910962104797363 }, "id": "GPKLONFDEH_565", "locus": "GCA000006625_00533" }, { "type": "cds", "sequence": "contig_1", "start": 313008, "stop": 313286, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGTGTTAGTTCGTTATTTACAATTACAAAATATTAATATTCAATTACCAGCAGATGCCTACCATGGGCAAGAAATTCATTTAGTTGCACAAACTTTATACCAAACTTATAAAAATCAATTTATTAATGTACGTTTAAATGAAAAATATGAAATTGATGATGATATTGCTAATCAAGAAATTAAAAATTTTGCAGTTAAATACTTATTAAATGAAATTAAAAACGATTTAGCATCAATTAACACATTCATTGATACTTATACTTCAGAAAATTGA", "aa": "MSVLVRYLQLQNINIQLPADAYHGQEIHLVAQTLYQTYKNQFINVRLNEKYEIDDDIANQEIKNFAVKYLLNEIKNDLASINTFIDTYTSEN", "aa_hexdigest": "2b88246dbe031bf8b54049773c6e2e9c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10812.019499999988, "isoelectric_point": 4.7395421981811525 }, "id": "GPKLONFDEH_566", "locus": "GCA000006625_00534" }, { "type": "cds", "sequence": "contig_1", "start": 313500, "stop": 313709, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGGGGTACAGATCATTTAGGTTATATTGCGCGTTTAAAAGCGGCAATGAATGCTTTAGGTTATGATCCAAATAATTTAGAAATTGTTTGTGCTCAAGTAATGAAATTAGTTAAAAATAATCAAGAATTTAAACTTTCAAAAAGGAGCGGACAATCATTAACAATCAAGGATTTAGTTGAAATTATTGGTAAAGATGCATTACGTTGA", "aa": "MWGTDHLGYIARLKAAMNALGYDPNNLEIVCAQVMKLVKNNQEFKLSKRSGQSLTIKDLVEIIGKDALR", "aa_hexdigest": "8262bf216fe6cdf5c768110ad09c76f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7732.980199999996, "isoelectric_point": 9.250649070739744 }, "id": "GPKLONFDEH_567", "locus": "GCA000006625_00535" }, { "type": "cds", "sequence": "contig_1", "start": 313728, "stop": 314078, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGAATTCGCATGTTATTATTGATGTAGATATTGCCTTATCAAAAAATAACAATAATCCTTTATATTATGTTCAATACGCACATGCTCGTGCCAATCAAGTATTAAATAAACAAGTTTATGAATTAGATTTTAAAACTGATCTATTAACAGAAACACGTGAACGTGAATTGTTGAATCAACTTCATTTCTATAAACAAACAATTGCTAATGCTGCAAATAATCGTGAACCACACCGAATTAGTAATTATTTATATGACTTAGCTCAAATTTTTCATAATTATTATGCAAATGTTAAAATTAATAATGATAATAATAAAGTTCTATCAGCACAACGTTATACATTAGTATGA", "aa": "MNSHVIIDVDIALSKNNNNPLYYVQYAHARANQVLNKQVYELDFKTDLLTETRERELLNQLHFYKQTIANAANNREPHRISNYLYDLAQIFHNYYANVKINNDNNKVLSAQRYTLV", "aa_hexdigest": "39b3d7ab009f7186e5d84049e1b58896", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 96, "aa_cov": 0.8017241379310345, "hmm_cov": 0.9895833333333334, "evalue": 1.0912617649713437e-24, "score": 82.39945220947266, "start": 24, "stop": 116 } ], "seq_stats": { "molecular_weight": 13716.192899999978, "isoelectric_point": 7.971789741516113 }, "id": "GPKLONFDEH_568", "locus": "GCA000006625_00536" }, { "type": "cds", "sequence": "contig_1", "start": 314150, "stop": 314581, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGAATAGTTTAAATAAAAAAAGTATAAAAGATCTTGATGTAAATGGTAAAACAATAGTTTTACATTTAGATTTAAATGTTGTTGTTGATTATGAAAATAAACGCATATTAAATGATCGTAAATTACGAGCATCACTACCAACTATTAATTATTTAATTAATCATAACGCTAAAATTGTTATTTTAAGTCATTTAGGTCGAATTAAAACCTTAGCAGATAAACAAAGTGGTAAATATAGTTTAGAAATTATTGTTGATGAACTCCGTAATCGTGTATCCAAGAATGTTAATCGTGTTGTATTTTCACCTTTAAATTATGGAGAAACGGTCGTACAAATGGTTAATGATTTAGAAGAAAGAGACATTTTAATTTTAGAAAATACGCGTTATTGTGATATTAGTGATGAAGGTGAATATGTTGGGCTTGAATGA", "aa": "MNSLNKKSIKDLDVNGKTIVLHLDLNVVVDYENKRILNDRKLRASLPTINYLINHNAKIVILSHLGRIKTLADKQSGKYSLEIIVDELRNRVSKNVNRVVFSPLNYGETVVQMVNDLEERDILILENTRYCDISDEGEYVGLE", "aa_hexdigest": "b6590c72885b818332a669a70c6b8e40", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 124, "aa_cov": 0.8671328671328671, "hmm_cov": 0.9354838709677419, "evalue": 1.3676408715729656e-29, "score": 98.84573364257812, "start": 9, "stop": 132 } ], "seq_stats": { "molecular_weight": 16408.64179999999, "isoelectric_point": 6.311708641052246 }, "id": "GPKLONFDEH_569", "locus": "GCA000006625_00537" }, { "type": "cds", "sequence": "contig_1", "start": 314615, "stop": 315418, "strand": "+", "frame": 2, "gene": "pgk", "product": "Phosphoglycerate kinase", "db_xrefs": [ "COG:COG0126", "COG:G", "EC:2.7.2.3", "GO:0004618", "GO:0005524", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0043531", "KEGG:K00927", "SO:0001217", "UniRef:UniRef50_Q9PQL2", "UniRef:UniRef90_Q9PQL2" ], "nt": "ATGCTAGGGGATATTTTTATTGATGATGCATATGGAGTTGCTCATCGACAATTATCATCAAATTATCAAACAGCTAAATTTGCTAAAAAAAGTGCATTAGGTTTTTTAATTGTTAATGAAATTAACCATCTAGATATTGCTTTAGAAGTTCCTAAAAGTCCGTATTTAGCTTTAATAGGTGGTAACCGTGTTGCTGATAAAATTGCAGCAATTGAAGTATTATGTGAACGGGCTGATCAAGTTATTATTGGGGGAGGATTAGTCTATACATTTTTATATGCTCAAGGGTATAACGTTGGTTTAAATTTAGTAGAACGCAATATGATTGATGATTGCAATAATATATTAGAAAAATACGGTAAAAAAATTTTAATTTGTTTTGATTTTTTATGTAACAATGATTTTAGTGATACAAGACCGATTTATCGTAAAATAGATGAAGGTCTTGAAGGGTTATATGGTTTAGATATTGGTAAAAGAAGTTTAAAATTTATTAAACATGAAATTTATCGTTCTAAAACTATTTTACTAAATGGTCCCTTTGGTGTTATTGAAAATATTAAGAATTATGCACAAGGTACTACTGAAATATGTAAATCAATGGCAAAACAAACAACACGTGGCGCTTATACAATTATATGTGATATCGATACTTCGAGTCATGCTGAATACCTAGGATTAGATAAATATATTAATTTTATATCTACTGGTGGGGGCGCTAGCTTATCATATATTGAAGAAGGAGTTCTTGATGGTCTTGAAACCATTGATAATGTTCCTTTAAATGTTTTAAAGAAGGAGTAA", "aa": "MLGDIFIDDAYGVAHRQLSSNYQTAKFAKKSALGFLIVNEINHLDIALEVPKSPYLALIGGNRVADKIAAIEVLCERADQVIIGGGLVYTFLYAQGYNVGLNLVERNMIDDCNNILEKYGKKILICFDFLCNNDFSDTRPIYRKIDEGLEGLYGLDIGKRSLKFIKHEIYRSKTILLNGPFGVIENIKNYAQGTTEICKSMAKQTTRGAYTIICDIDTSSHAEYLGLDKYINFISTGGGASLSYIEEGVLDGLETIDNVPLNVLKKE", "aa_hexdigest": "aa69749a5943d1d27c2325c9aaf0e371", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "pgk" ], "truncated": "5-prime", "pseudogene": { "start": 314315, "stop": 315418, "observations": { "mutation": [ 314579 ], "stop": [ 314579 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQL2", "query_cov": 1.0, "subject_cov": 0.6327014218009479, "identity": 1.0, "score": 536.0, "evalue": 2.59e-190, "gene_start": 1, "gene_end": 267, "reference_start": 156, "reference_end": 422, "reference_sequence": "MNSLNKKSIKDLDVNGKTIVLHLDLNVVVDYENKRILNDRKLRASLPTINYLINHNAKIVILSHLGRIKTLADKQSGKYSLEIIVDELRNRVSKNVNRVVFSPLNYGETVVQMVNDLEERDILILENTRYCDISDEGEYVGLEWDGSEILGQFWGMLGDIFIDDAYGVAHRQLSSNYQTAKFAKKSALGFLIVNEINHLDIALEVPKSPYLALIGGNRVADKIAAIEVLCERADQVIIGGGLVYTFLYAQGYNVGLNLVERNMIDDCNNILEKYGKKILICFDFLCNNDFSDTRPIYRKIDEGLEGLYGLDIGKRSLKFIKHEIYRSKTILLNGPFGVIENIKNYAQGTTEICKSMAKQTTRGAYTIICDIDTSSHAEYLGLDKYINFISTGGGASLSYIEEGVLDGLETIDNVPLNVLKKE" }, "paralog": false, "identity": 0.997275204359673, "score": 728.0, "evalue": 2.9e-268, "description": "Internal stop codon at 314579. Nonsense mutation around 314579.", "psc": { "uniref90_id": "UniRef90_Q9PQL2", "gene": "pgk", "product": "Phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_Q9PQL2", "cog_id": "COG0126", "cog_category": "G", "kegg_orthology_id": "K00927", "go_ids": [ "GO:0005829", "GO:0043531", "GO:0005524", "GO:0004618", "GO:0006094", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQL2", "EC:2.7.2.3", "UniRef:UniRef50_Q9PQL2", "COG:COG0126", "COG:G", "KEGG:K00927", "GO:0005829", "GO:0043531", "GO:0005524", "GO:0004618", "GO:0006094", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQL2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQL2" ], "product": "Phosphoglycerate kinase" } }, "id": "GPKLONFDEH_570", "locus": "GCA000006625_00538" }, { "type": "cds", "sequence": "contig_1", "start": 315422, "stop": 316513, "strand": "+", "frame": 2, "gene": "frvX", "product": "Endo-1,4-beta-glucanase", "db_xrefs": [ "COG:COG1363", "COG:EG", "RefSeq:WP_006688850.1", "SO:0001217", "UniParc:UPI00000C1BFE", "UniRef:UniRef100_Q9PQL1", "UniRef:UniRef50_Q9PQL1", "UniRef:UniRef90_Q9PQL1" ], "nt": "ATGAGTAAATTTAACGAAAAAGAAATTAGAAATAAAGCAATTGAATATATGGAAATATATGGTATGTCACGTCATGAAGAACGTGTTGCTTCCAAACTAAAAGCATCATTAAAAGATGCGGGAGTTGATTATGAACGTGATAATTTAGGTTCAATTATTTTTAAAAAAACAAATTCAAATAAAGGACCTAAAATCTTAATTGCTACACACATGGATGAAGTTGGTTTTGTAGTTCAACAAATTCTTGATAATGGTCAATTACTATTATCAATGGTAGGTGGTGTATGGCCTAATATTGCTATTGGATCAGTAGCAAAAGTGTATGTTGATGAACAACGTCAATATACTGGTGTATTTGGCCATACTTCAATTCATATCTTAGAACCTGAAGCACGCAGTAAAGCAGTTCCAGTTAAAGAATTATTTGTTGATTGCGGTTTTAATTCAAAACAACAAGCTATTGATTTAGGAATTGAAATAGGTACGGAAGTTTATATGGAAGGACCAACTTTAAATTTTTATGATGAAAATTATATTGTTGGTAAAGCTGTTGATAATCGTGTAAGTGTTGCGGTTTTAGATTTATTAATACATAGTTTAAAGGACAAAGTAATTCCCAACCAAACTTATTTTGCTGCAACAGTGCAAGAAGAAGTTGGATTACGTGGCGCAAAAACAGTTGTTTCAAAAGTAAAACCTGATATTGGGATTGTTATTGATACAACAACATCACACGATACATACAAGTGTCCAGAGGGTGATACAAAATTAAATGATGGTGTATGTATTAGAATTAAAGATGGTGGAACTTTAGTTAATCCTAAATTAGTAAAATATTTTGAAGAATTAGCTATTAAACACAACATTCCTTTATATAAATATGTTGCACGAGGAGGCGGGACTGATGCTGAAGAATTGCAATATGGGCCTAATGGTGGAGTATTAACAATTGGTTTGTCTATCCCACAACGTTATTTACATGCTCCAATTGGCGTAGCAACGGTTAAAGATATAAAAGCAGCCTTTGACTTAGTACGTGAGTTTTTAGAAGTATTTGATGAAAACGAATTTGAAAAAGTAAAATTTAAATAA", "aa": "MSKFNEKEIRNKAIEYMEIYGMSRHEERVASKLKASLKDAGVDYERDNLGSIIFKKTNSNKGPKILIATHMDEVGFVVQQILDNGQLLLSMVGGVWPNIAIGSVAKVYVDEQRQYTGVFGHTSIHILEPEARSKAVPVKELFVDCGFNSKQQAIDLGIEIGTEVYMEGPTLNFYDENYIVGKAVDNRVSVAVLDLLIHSLKDKVIPNQTYFAATVQEEVGLRGAKTVVSKVKPDIGIVIDTTTSHDTYKCPEGDTKLNDGVCIRIKDGGTLVNPKLVKYFEELAIKHNIPLYKYVARGGGTDAEELQYGPNGGVLTIGLSIPQRYLHAPIGVATVKDIKAAFDLVREFLEVFDENEFEKVKFK", "aa_hexdigest": "38ee7e2c4247905f8c6aabd5608bceee", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1BFE", "ncbi_nrp_id": "WP_006688850.1", "uniref100_id": "UniRef100_Q9PQL1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1BFE", "RefSeq:WP_006688850.1", "UniRef:UniRef100_Q9PQL1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQL1", "uniref90_id": "UniRef90_Q9PQL1" }, "psc": { "uniref90_id": "UniRef90_Q9PQL1", "gene": "FrvX", "product": "Endo-1,4-beta-glucanase", "uniref50_id": "UniRef50_Q9PQL1", "cog_id": "COG1363", "cog_category": "EG" }, "pscc": { "uniref50_id": "UniRef50_Q9PQL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQL1" ], "product": "Endo-1,4-beta-glucanase" }, "genes": [ "frvX" ], "id": "GPKLONFDEH_571", "locus": "GCA000006625_00539" }, { "type": "cds", "sequence": "contig_1", "start": 316719, "stop": 317306, "strand": "+", "frame": 3, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0006139", "KEGG:K03784", "SO:0001217", "UniRef:UniRef50_P47295", "UniRef:UniRef90_Q9PQL0" ], "nt": "ATGCTATGCTATACAGGAACATATAAAGGCAAAAAAATATCAATTATGGGCCATGGTATGGGAATTCCTTCGATCGGAATTTATTCATATGAATTATATAAATTTTATGAAGTTGAAACAATTATTCGTGTTGGTTCAACTGGTTCATATAAAAAAGATTTGAATGTTAATGATGTGGTTTTAGTTGAAAAATCATATAGTGATTCTACATTCGCATCACTGATTGGTGCACAAGTTGGCGATGATAAGGTTTTATTACCATCAAGAGAAGTTAATAATTTAATTAAAAATACTGCGAAGGAATTAAATTTAGAATTATCATTAGCAACTTGTCATGCTTCTGATGTGTTTTATAATAATGATTTTCAAACATTAGATGAAATTATTCATCGAACTAAATCCGATTGTGTTGATATGGAATCATTTGGTTTATTCGCAAATGCACAAATTTTAAATAAAAATGCAGCAACTTTACTAACAGTTAGTGATTCATTGATTACAGGTGATGCACTATCACCCGCAGAGCGTGCTAATACTTTTAAAAAAATGGTAACATTAGCCTTAGAAAGCGCAATTAAATTACTTTAA", "aa": "MLCYTGTYKGKKISIMGHGMGIPSIGIYSYELYKFYEVETIIRVGSTGSYKKDLNVNDVVLVEKSYSDSTFASLIGAQVGDDKVLLPSREVNNLIKNTAKELNLELSLATCHASDVFYNNDFQTLDEIIHRTKSDCVDMESFGLFANAQILNKNAATLLTVSDSLITGDALSPAERANTFKKMVTLALESAIKLL", "aa_hexdigest": "03798f125b1aa80b3892e6150efcbc52", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQL0", "query_cov": 1.0, "subject_cov": 0.8158995815899581, "identity": 0.9129999999999999, "score": 351.0, "evalue": 3.71e-121, "valid": true, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_P47295", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0006139" ] }, "pscc": { "uniref50_id": "UniRef50_P47295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47295" ], "product": "Purine nucleoside phosphorylase DeoD-type" }, "genes": [ "deoD" ], "id": "GPKLONFDEH_572", "locus": "GCA000006625_00540" }, { "type": "cds", "sequence": "contig_1", "start": 317402, "stop": 317794, "strand": "+", "frame": 2, "gene": "hup", "product": "DNA-binding protein HU", "db_xrefs": [ "COG:B", "COG:COG0776", "GO:0003677", "GO:0030261", "GO:0030527", "RefSeq:WP_006688857.1", "SO:0001217", "UniParc:UPI0000128F24", "UniRef:UniRef100_Q9PQK9", "UniRef:UniRef50_Q9PQK9", "UniRef:UniRef90_Q9PQK9" ], "nt": "ATGAATGCAAAAATTAAAGCCAAAACACGTTCACAAATGATAGATGAATTATCAAAAATGTTAAATATTGAAAAAAAACAAACAAAAGCATTTATGGATACTTACGAAGCTTTTTTAATTCTTGAATTATCACGAGCTAAAGAAGTTCGTTTAGGAAATATTGGAAAATTCAAGGTTAGTGTACGTGCAGAACGTAAGGGAATTAACCCTAAAACGGGTGAAACTGTAATTATTCCCGAGAAAACAATTCCTAAATTTACTTTTACTAAAGGAATTAAAGAAATTATTAATGCTGGTATTTCAATTGATAATGAAAGAGTATCGATTGATGATAATGATTTTGATGATGACGATGAATTTGTAGAAGAGTACATTGTATCAGAAAATAATTAA", "aa": "MNAKIKAKTRSQMIDELSKMLNIEKKQTKAFMDTYEAFLILELSRAKEVRLGNIGKFKVSVRAERKGINPKTGETVIIPEKTIPKFTFTKGIKEIINAGISIDNERVSIDDNDFDDDDEFVEEYIVSENN", "aa_hexdigest": "5a394bd5e270e9dae20f6c81205e0674", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000128F24", "ncbi_nrp_id": "WP_006688857.1", "uniref100_id": "UniRef100_Q9PQK9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000128F24", "RefSeq:WP_006688857.1", "UniRef:UniRef100_Q9PQK9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQK9", "uniref90_id": "UniRef90_Q9PQK9" }, "psc": { "uniref90_id": "UniRef90_Q9PQK9", "gene": "hup", "product": "DNA-binding protein HU", "uniref50_id": "UniRef50_Q9PQK9", "cog_id": "COG0776", "cog_category": "B", "go_ids": [ "GO:0003677", "GO:0030527", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK9" ], "product": "DNA-binding protein HU" }, "genes": [ "hup" ], "id": "GPKLONFDEH_573", "locus": "GCA000006625_00541" }, { "type": "cds", "sequence": "contig_1", "start": 317894, "stop": 318088, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGAATTAGTTAAAAAAAAATTAGATGATTTATATGATTTTCTTAAACAAAAAAAATATTCAAGCCAAACCATCGAATTAGTCCATAAATCATTTGATTTTGCAAACTATTATCATGGTGATCAAACAAGAAAGTCGGGAGAACCATATATCATTCATCCAATTGCAACTGTACGAATTTTAGCTTCATGA", "aa": "MNELVKKKLDDLYDFLKQKKYSSQTIELVHKSFDFANYYHGDQTRKSGEPYIIHPIATVRILAS", "aa_hexdigest": "6620b8e8205a79ee86a4a08fa74828ec", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7533.5297, "isoelectric_point": 8.927146720886231 }, "id": "GPKLONFDEH_574", "locus": "GCA000006625_00542" }, { "type": "cds", "sequence": "contig_1", "start": 318092, "stop": 318565, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAAAGTACATTAGTAGCAGGTATGTTACACGATGTTTTAGAAGACACAAATTGTCCAGAAGAAGTTATGGAAAAAACTTTTGGTAAAGAAGTGACGCAACTTGTTTGTTTTGTAACAAAAGTATCTCTTTATTCAAAAAATCGACGTAATAAAGTTTCACACACCAATCTTGAAGAAAAGTATTCAATTCAAGTGTTTATGTCAATGACACAAGACATTCGTGCTATGATTATTAAAATTGCTGATCGTTATCATAATATGCAAACTATTCAATACTTACGTTTAGAAAAAGCGAAAAGAATTGCAACAGAAACACTAGAAATTTACGCAAATATTTCTGGAAGATTAGGGATGTATCGTGTTAAAACAGATTTATTAGATATGTCTTTTAAAATTTTAGACCCACAAAATTATCAATTAGTAAACGATTCAATTAATAAATTGGTTGAAAACAATCAAAAAAAATGA", "aa": "MDESTLVAGMLHDVLEDTNCPEEVMEKTFGKEVTQLVCFVTKVSLYSKNRRNKVSHTNLEEKYSIQVFMSMTQDIRAMIIKIADRYHNMQTIQYLRLEKAKRIATETLEIYANISGRLGMYRVKTDLLDMSFKILDPQNYQLVNDSINKLVENNQKK", "aa_hexdigest": "a0593bb604bb0db86bfd6bb43dd90272", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18333.016400000004, "isoelectric_point": 7.733365440368652 }, "id": "GPKLONFDEH_575", "locus": "GCA000006625_00543" }, { "type": "cds", "sequence": "contig_1", "start": 318880, "stop": 319167, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAATTATTTATATCGTATTTATATGCTACATCTAATAATGTTGAACCATTTGAAACAGTGATTCATTTATTAGAATTAAGAATTAGTACATCAAAAATAGTTCCGGTTGTGACATCTTTAATAGTTTTAATTCCTTTATCTGTGCTCAATAAAAAATCACGCAATAAAACATTATTAACTGTTAATAAAATTTCAGTTTTATTATCTTTTTGTTCTTTATATTTTCAGTGTGCTGCTAAACCATTATTAGCAAATAAATTCATTTTTGTTGTTCTGATTTGA", "aa": "MKKLFISYLYATSNNVEPFETVIHLLELRISTSKIVPVVTSLIVLIPLSVLNKKSRNKTLLTVNKISVLLSFCSLYFQCAAKPLLANKFIFVVLI", "aa_hexdigest": "e685aedb8bd2fd5975a59a0a04e48dd4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10713.91509999999, "isoelectric_point": 9.885920524597168 }, "id": "GPKLONFDEH_576", "locus": "GCA000006625_00544" }, { "type": "cds", "sequence": "contig_1", "start": 319412, "stop": 319588, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGAAGAGCATGCTATTAAAAGACTAGAAAAAGAATTTCAAGTTAATAGTATCAATACTTTTTTAGATAATATTGCTAATACTGACATTGATTTACAAACAAAATATAAAGTATTTTCAGAAAATAAACAAGTTTCACGTGAAGCAATTAATACAATTAAAAATCAAGCATGA", "aa": "MSEEHAIKRLEKEFQVNSINTFLDNIANTDIDLQTKYKVFSENKQVSREAINTIKNQA", "aa_hexdigest": "003d09da262a08e63fea20867304e3cf", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6756.479400000002, "isoelectric_point": 5.69995059967041 }, "id": "GPKLONFDEH_577", "locus": "GCA000006625_00545" }, { "type": "cds", "sequence": "contig_1", "start": 319631, "stop": 319783, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTTTACAGATTTTGAAATTTCGCCTTGTTGTTCTAAAATTCCTGGAATTAATTGTGTTGCTAAAATTGTTCGCAATAAAGTAGAAATTCACCGCAATGATTGTTCTAAAGTTAAAAATTCTCGCTCAAAAACAATTGTAATTGAATGA", "aa": "MYFTDFEISPCCSKIPGINCVAKIVRNKVEIHRNDCSKVKNSRSKTIVIE", "aa_hexdigest": "604153c230d2d81866b10177baebab30", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5715.673200000001, "isoelectric_point": 9.098245811462402 }, "id": "GPKLONFDEH_578", "locus": "GCA000006625_00546" }, { "type": "cds", "sequence": "contig_1", "start": 319892, "stop": 320050, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAAATATTTCAAAAGTTGATATTAGTAAAAATAAGACTGCACGCACTCATGATACTTTTTTAAAAATTTATGTAAAAAACTTAGATCACTTACAAAAGATTATTTTAGAACTTCAAGCTAAAAATGTTATTAATGAATGGAGATTGATCTAA", "aa": "MKANISKVDISKNKTARTHDTFLKIYVKNLDHLQKIILELQAKNVINEWRLI", "aa_hexdigest": "09193856b3603bc7d5dae58958366b33", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6147.199999999998, "isoelectric_point": 9.90029697418213 }, "id": "GPKLONFDEH_579", "locus": "GCA000006625_00547" }, { "type": "cds", "sequence": "contig_1", "start": 320050, "stop": 320745, "strand": "+", "frame": 1, "gene": "pyrG", "product": "CTP synthase", "db_xrefs": [ "COG:COG0504", "COG:F", "EC:6.3.4.2", "GO:0003883", "GO:0004359", "GO:0005524", "GO:0005829", "GO:0006221", "GO:0006241", "GO:0019856", "GO:0042802", "GO:0044210", "GO:0046872", "KEGG:K01937", "SO:0001217", "UniRef:UniRef50_Q9PQK7", "UniRef:UniRef90_Q9PQK7" ], "nt": "ATGAAACATATACAAAAAACAAAATTTATTTTTGTAACTGGTGGTGTTTATTCATCATTAGGTAAAGGGGTTAGTGCTAGTAGTATTGGTCGAATTCTTGTTGAATTAGGATATAGTGTTGCAATGCAAAAGCTTGATCCATATTTAAATATTGATCCCACTTATTTATCACCATTACAACATGGAGAAGTATTTGTTACAAAAGACGGTAAAGAAGCAGATTTAGATTTAGGTACTTATGAACGTTTTATTAATGCTGATTTAAATAAATATGCGTCAGTAACTTCAGGAAAAATTTATTATGAAATTTTAACAAAAGAGCGTGAAAATGGTTTTGACGGTAAAACTGTTCAAACTATTCCACATGTCACATCTGCAGTTATTGATTACATTAAAAAAATTAAAGATAGTTTAAAAACTGATTTTATTATTGTTGAAATTGGAGGAACAATTGGAGATATTGAATCGCTTCCATTTATTGAAGCTATTAGTCAATTTAAAACAATTTATGGTGTAAATAATGTAATGTTTATTCACTGTTCACCGCTTATTTATATTGAAAAAGTAGGTGAATTAAAAACAAAACCAACTCAACATAGCGTTAAGACGTTACGTTCATTAGGAATTAATTTAGATTTATTGTTATTAAGAACTAATCAAAAATTAGATGAAGTAACGATTAAAAAATTAGCATGA", "aa": "MKHIQKTKFIFVTGGVYSSLGKGVSASSIGRILVELGYSVAMQKLDPYLNIDPTYLSPLQHGEVFVTKDGKEADLDLGTYERFINADLNKYASVTSGKIYYEILTKERENGFDGKTVQTIPHVTSAVIDYIKKIKDSLKTDFIIVEIGGTIGDIESLPFIEAISQFKTIYGVNNVMFIHCSPLIYIEKVGELKTKPTQHSVKTLRSLGINLDLLLLRTNQKLDEVTIKKLA", "aa_hexdigest": "a155d0e609022eab56c885fec284f8f7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI000382712F", "query_cov": 0.974025974025974, "subject_cov": 0.8211678832116789, "identity": 0.573, "score": 261.0, "evalue": 1.89e-84, "valid": false, "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_A0A381VMU8", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0006221", "GO:0006241" ] }, "genes": [ "pyrG" ], "truncated": "3-prime", "pseudogene": { "start": 320035, "stop": 321048, "observations": { "mutation": [ 320743, 320893 ], "stop": [ 320743, 320893 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQK7", "query_cov": 1.0, "subject_cov": 0.4261992619926199, "identity": 1.0, "score": 452.0, "evalue": 6.9e-156, "gene_start": 1, "gene_end": 231, "reference_start": 6, "reference_end": 236, "reference_sequence": "MEIDLMKHIQKTKFIFVTGGVYSSLGKGVSASSIGRILVELGYSVAMQKLDPYLNIDPTYLSPLQHGEVFVTKDGKEADLDLGTYERFINADLNKYASVTSGKIYYEILTKERENGFDGKTVQTIPHVTSAVIDYIKKIKDSLKTDFIIVEIGGTIGDIESLPFIEAISQFKTIYGVNNVMFIHCSPLIYIEKVGELKTKPTQHSVKTLRSLGINLDLLLLRTNQKLDEVTIKKLAWSCGLDIDMIFAAYDVESVYLLPNVLFEQGIHKTILDFFSLPLKNDNINSWIDFTDKITTFKKHNLVIGLVGKYVELPDAYKSVLASLELAAIELNIDLKIKYIQPQNLNENNINEELKKINGIVIPSIAGSIKGWPGALLAASYARKNNIPFLAVGTGVNIGIGEFINNVLKLPIEFINLGNGDFSFLKDAFVKNEIENYRIGEYCSNIQANTITSQIYLKQNQLNERHRHHFEFNNHYINNYFLNQNWKIGAISVDNNYIDVLEYTKNHFYVLTIFNPEYTSKPSKANPYFINLLKMSLKIKES" }, "paralog": false, "identity": 0.9940652818991098, "score": 652.0, "evalue": 2.67e-237, "description": "Internal stop codon at 320743, 320893. Nonsense mutation around 320743, 320893.", "psc": { "uniref90_id": "UniRef90_Q9PQK7", "gene": "pyrG", "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_Q9PQK7", "cog_id": "COG0504", "cog_category": "F", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0004359", "GO:0005524", "GO:0005829", "GO:0006221", "GO:0006241", "GO:0019856", "GO:0042802", "GO:0044210", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQK7", "EC:6.3.4.2", "UniRef:UniRef50_Q9PQK7", "COG:COG0504", "COG:F", "KEGG:K01937", "GO:0003883", "GO:0004359", "GO:0005524", "GO:0005829", "GO:0006221", "GO:0006241", "GO:0019856", "GO:0042802", "GO:0044210", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK7" ], "product": "Putative CTP synthase" } }, "id": "GPKLONFDEH_580", "locus": "GCA000006625_00548" }, { "type": "cds", "sequence": "contig_1", "start": 320767, "stop": 320895, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGCTGCTTATGATGTTGAGTCAGTTTATTTATTACCAAATGTTTTGTTTGAGCAAGGGATTCATAAAACTATTTTAGATTTTTTTAGTCTACCTTTAAAAAATGATAATATTAATTCATGA", "aa": "MIFAAYDVESVYLLPNVLFEQGIHKTILDFFSLPLKNDNINS", "aa_hexdigest": "e0c56e8b4c446bccbc32e4de4f7fc0dc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4830.5089, "isoelectric_point": 4.427268791198731 }, "id": "GPKLONFDEH_581", "locus": "GCA000006625_00549" }, { "type": "cds", "sequence": "contig_1", "start": 321208, "stop": 321492, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAACTGGCGTTAATATTGGTATTGGTGAATTCATTAATAATGTACTAAAACTTCCAATTGAATTTATTAATTTAGGCAATGGTGATTTTAGTTTTTTAAAAGACGCTTTCGTTAAAAATGAAATTGAAAATTATCGAATTGGAGAGTATTGTTCTAATATTCAAGCTAACACTATAACAAGCCAAATTTATCTCAAACAAAATCAATTGAACGAGCGCCATCGTCATCATTTTGAATTTAACAATCATTATATTAATAATTATTTTTTAAATCAAAATTGA", "aa": "MGTGVNIGIGEFINNVLKLPIEFINLGNGDFSFLKDAFVKNEIENYRIGEYCSNIQANTITSQIYLKQNQLNERHRHHFEFNNHYINNYFLNQN", "aa_hexdigest": "63eb5e7a4d92ea276826962db6e577a4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11027.180599999992, "isoelectric_point": 6.026490592956542 }, "id": "GPKLONFDEH_582", "locus": "GCA000006625_00550" }, { "type": "cds", "sequence": "contig_1", "start": 321511, "stop": 321663, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAATAATTATATTGATGTTTTAGAATACACAAAAAATCATTTTTATGTTTTAACAATTTTTAATCCAGAATATACATCAAAACCTTCAAAGGCAAATCCTTATTTTATTAATTTATTAAAGATGAGTTTAAAAATAAAAGAAAGTTAG", "aa": "MDNNYIDVLEYTKNHFYVLTIFNPEYTSKPSKANPYFINLLKMSLKIKES", "aa_hexdigest": "9509f6e245a01be79dbbb99aa40ea3b2", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5976.825, "isoelectric_point": 7.983909797668457 }, "id": "GPKLONFDEH_583", "locus": "GCA000006625_00551" }, { "type": "cds", "sequence": "contig_1", "start": 321667, "stop": 321894, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "ATGATTAATTACAGTAAACCACGAGGTACTATAGATTTATATAATAAAGAAATGAATGATTTTAAATTACTTGAAAATTTTTTATTATCAACTACTAAAAGATATGGCTTTCAGCAAATTAAAACACCTGTTTTTGAATTTGCAGAACTCTTTATGAAATCAGCTGGTGAAAGTTCAGATTTAGTTTCAAAAGAAATGTATTTATTTAAAGATAAATCAGATCGCTGA", "aa": "MINYSKPRGTIDLYNKEMNDFKLLENFLLSTTKRYGFQQIKTPVFEFAELFMKSAGESSDLVSKEMYLFKDKSDR", "aa_hexdigest": "7d5457290739b05c43af2de906159c27", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 66, "aa_cov": 0.88, "hmm_cov": 0.9393939393939394, "evalue": 2.0811492947593864e-07, "score": 26.050662994384766, "start": 10, "stop": 75 } ], "seq_stats": { "molecular_weight": 8875.098699999997, "isoelectric_point": 7.925501441955567 }, "id": "GPKLONFDEH_584", "locus": "GCA000006625_00552" }, { "type": "cds", "sequence": "contig_1", "start": 321985, "stop": 322197, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30", "PFAM:PF13393.11" ], "nt": "ATGTATTTTGAGCCTTGTTTTCGTTATGAACGCCCTCAAGCTGGACGTCAACGCCAATTTCATCAATTTGGTGTTGAAGTGTTGGGAACAAAAAATATTTATTATGATTTTGAATTAATTGCTTTAGCTGATACAATTTTAAAAAAATTAATGATATCTAATTATATTTTAGAAATTAATTATATCTCATCTCCTCATAATCGTTCATTATGA", "aa": "MYFEPCFRYERPQAGRQRQFHQFGVEVLGTKNIYYDFELIALADTILKKLMISNYILEINYISSPHNRSL", "aa_hexdigest": "c70dd34948599a594b39b60dc81c3712", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 65, "aa_cov": 0.8428571428571429, "hmm_cov": 1.0, "evalue": 2.5198113224911225e-09, "score": 33.01798629760742, "start": 2, "stop": 60 }, { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 61, "aa_cov": 0.8714285714285714, "hmm_cov": 1.0, "evalue": 7.349205303792991e-11, "score": 37.388572692871094, "start": 1, "stop": 61 } ], "seq_stats": { "molecular_weight": 8391.572999999993, "isoelectric_point": 7.888045310974121 }, "id": "GPKLONFDEH_585", "locus": "GCA000006625_00553" }, { "type": "cds", "sequence": "contig_1", "start": 322207, "stop": 322845, "strand": "+", "frame": 1, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "COG:COG0124", "COG:J", "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "KEGG:K01892", "SO:0001217", "UniRef:UniRef50_Q9PQK6", "UniRef:UniRef90_Q9PQK6" ], "nt": "TTGCAAGAATATTTTCATTTACATCGTACTGAATTAACTCCATTAAGTCAAGAACGTATTACAACCAATCCTTTGCGTATTTTAGATGATAAATTAGAATCTCAAAAACTAGTTGTAAAACAAGCTCCAAAAATTAGTCATTTTTTATCAAATGAAGAAAAAGAAGAATTTAATCTAATTAAAAAAATGCTTGATGATTATAACATTAAATATTATGTTAACGAAGGATTAGTAAGAGGTTTGGATTATTATAGTGGATTAGTATTTGAATTTATTTCAACTTCACCTAAATTATTAGGTCAATCAACAATTATAGGTGGAGGCCGATATGGTGAATTAATTAAACAAACAGGTGGCCCTAATTATGAAGGTATTGGTTTTGGTATAGGAATTGAACGTTTATTAATAGCTTTATCTGAAACCAATAAAAATATTTTAAACACTGATGATGATAAATATTTAATTGCATTTTTTGATAAAGAATTAGAGAATGAAGCTATAAAACTAACTCAAATATTACGTATCAATAATCAATTAAATGTTGACATTATCTTAAGTTCAACTAAAGCTGACAAAATTTTTAAGTTAGCACAACGTTTAAATGTAAAAAAGCTTATAATATTAGCTAAAAAAGAATGA", "aa": "MQEYFHLHRTELTPLSQERITTNPLRILDDKLESQKLVVKQAPKISHFLSNEEKEEFNLIKKMLDDYNIKYYVNEGLVRGLDYYSGLVFEFISTSPKLLGQSTIIGGGRYGELIKQTGGPNYEGIGFGIGIERLLIALSETNKNILNTDDDKYLIAFFDKELENEAIKLTQILRINNQLNVDIILSSTKADKIFKLAQRLNVKKLIILAKKE", "aa_hexdigest": "bde7990378c3056252f0deb41aee9653", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "hisS" ], "truncated": "both", "pseudogene": { "start": 321907, "stop": 322929, "observations": { "mutation": [ 322195, 322843 ], "stop": [ 322195, 322843 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQK6", "query_cov": 1.0, "subject_cov": 0.5047619047619047, "identity": 0.995, "score": 403.0, "evalue": 7.58e-139, "gene_start": 1, "gene_end": 212, "reference_start": 181, "reference_end": 392, "reference_sequence": "MINYSKPRGTIDLYNKEMNDFKLLENFLLSTTKRYGFQQIKTPVFEFAELFMKSAGESSDLVSKEMYLFKDKSDRWLALRPEGTAGVIRAVVENKLLLNHPLPLKLMYFEPCFRYERPQAGRQRQFHQFGVEVLGTKNIYYDFELIALADTILKKLMISNYILEINYISSPHNRSLWVKSLQEYFHLHRTELTPLSQERITTNPLRILDDKLESQKLVVKQAPKISHFLSNEEKEEFNLIKKMLDDYNIKYYVNEGLVRGLDYYSGLVFEFISTSPKLLGQSTIIGGGRYGELIKQTGGPNYEGIGFGIGIERLLIALSETNKNILNTDDDKYLIAFFDKELENEAIKLTQILRINNQLNVDIILSSTKADKIFKLAQRLNVKKLIILAKKEWSDKKIILKDLLNFKQDLLSLDEIKGIR" }, "paralog": false, "identity": 0.9941176470588236, "score": 659.0, "evalue": 2.76e-242, "description": "Internal stop codon at 322195, 322843. Nonsense mutation around 322195, 322843.", "psc": { "uniref90_id": "UniRef90_Q9PQK6", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_Q9PQK6", "cog_id": "COG0124", "cog_category": "J", "kegg_orthology_id": "K01892", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQK6", "EC:6.1.1.21", "UniRef:UniRef50_Q9PQK6", "COG:COG0124", "COG:J", "KEGG:K01892", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQK6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK6" ], "product": "Histidine--tRNA ligase" } }, "id": "GPKLONFDEH_586", "locus": "GCA000006625_00554" }, { "type": "cds", "sequence": "contig_1", "start": 323019, "stop": 323972, "strand": "+", "frame": 3, "gene": "aspS", "product": "aspartate--tRNA ligase", "db_xrefs": [ "COG:COG0173", "COG:J", "EC:6.1.1.12", "GO:0000166", "GO:0003676", "GO:0004812", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006418", "GO:0006422", "GO:0016874", "SO:0001217", "UniRef:UniRef50_Q9PQK5", "UniRef:UniRef90_Q9PQK5" ], "nt": "ATGGGTAATTTAGTTTTTGTTGACCTAAAAGATCGATTTGGAATTGTACAAATTTTTGCAACAAAAAGTGATGGTGTTTTTAATGAATTAACACAACTTTCACGTGAGGATGTAATTAATGTTGAAGGTTTAGTTTTGTTGAGAAAAAACCCTAATAATGATTTAAAAACAGGTAGATTTGAAATTCATGTCAAAAAAATACTTATTTATTCAAAAGCAAAAACACCACCATTAATTATTGAAGATGAAACTGATGCAAATGAGGAAATTCGTTTTAGATATCGTTATTTAGATTTACGTCGTGATGTTAATTTAAAAATTTTTGAATTGCGAAGTAAAGTTTACCAAGCTTTTAGAAATTACTTATATTCACAAGACTTTATTGAAACTGAAACTCCTATTTTAGCAAAACCAACGCCAGAAGGTGCACGAGATTTTTATGTGCCAACAAGAACTAGAAAATTTTATGCTTTACCACAATCACCTCAAACTTTTAAACAATTATTAATGGTTGCGGGTTTTCAAAAATATTTTCAAATTACAAAATGTTTTCGTGATGAAGATTTAAGAAGCGATCGTCAACCAGAATTTACTCAAGTTGATATAGAATTATCATTTGCTGATGAAATAGAAATTCAGACATTAATTGAAAATTTATTAAAATATGTTTTTAAACAAACCATTAATGTTGATTTAACAACTCCTTTTATGCGAATGAGTTATGAGCAAGCGATAAATGATTATGGTAGTGATAAACCAGATTTACGTTTTGATTTGAAAATTAAAACACTTAACACATATTTTGAAAATTCAAAAACATTATTTTTTCAAAAAGCACTTTTAAATAATCAAAGTATTCGTGCAATTTTAGTACCTAATATTAATTTAAATAAAAAACAAGTACAAACTCTTGAAAAGTTTGCCAAAGACAAAGGTGCTAAAGGTTTAAGTTGA", "aa": "MGNLVFVDLKDRFGIVQIFATKSDGVFNELTQLSREDVINVEGLVLLRKNPNNDLKTGRFEIHVKKILIYSKAKTPPLIIEDETDANEEIRFRYRYLDLRRDVNLKIFELRSKVYQAFRNYLYSQDFIETETPILAKPTPEGARDFYVPTRTRKFYALPQSPQTFKQLLMVAGFQKYFQITKCFRDEDLRSDRQPEFTQVDIELSFADEIEIQTLIENLLKYVFKQTINVDLTTPFMRMSYEQAINDYGSDKPDLRFDLKIKTLNTYFENSKTLFFQKALLNNQSIRAILVPNINLNKKQVQTLEKFAKDKGAKGLS", "aa_hexdigest": "129d341a110d94677b533a1fb707cb0f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920AYN4", "query_cov": 0.8927444794952681, "subject_cov": 0.8397626112759644, "identity": 0.502, "score": 276.0, "evalue": 4.07e-88, "valid": false, "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.12" ], "uniref50_id": "UniRef50_A0A3N5JT57", "go_ids": [ "GO:0000166", "GO:0004812", "GO:0005524", "GO:0006418", "GO:0016874" ] }, "genes": [ "aspS" ], "truncated": "both", "pseudogene": { "start": 322935, "stop": 324275, "observations": { "mutation": [ 322998, 323970, 324192, 324204 ], "stop": [ 322998, 323970, 324192, 324204 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQK5", "query_cov": 1.0, "subject_cov": 0.5484429065743944, "identity": 1.0, "score": 620.0, "evalue": 3.34e-220, "gene_start": 1, "gene_end": 317, "reference_start": 29, "reference_end": 345, "reference_sequence": "MKVYCGYIGEEHLEKNVILNGWVKKVRKMGNLVFVDLKDRFGIVQIFATKSDGVFNELTQLSREDVINVEGLVLLRKNPNNDLKTGRFEIHVKKILIYSKAKTPPLIIEDETDANEEIRFRYRYLDLRRDVNLKIFELRSKVYQAFRNYLYSQDFIETETPILAKPTPEGARDFYVPTRTRKFYALPQSPQTFKQLLMVAGFQKYFQITKCFRDEDLRSDRQPEFTQVDIELSFADEIEIQTLIENLLKYVFKQTINVDLTTPFMRMSYEQAINDYGSDKPDLRFDLKIKTLNTYFENSKTLFFQKALLNNQSIRAILVPNINLNKKQVQTLEKFAKDKGAKGLSWISIKDEKIIDGSLLSIQEDHIIYKTIFKDFNLSTGSILLVADTFDIASQALGLVRINLASILNLKKPNIFKFVWIIDWPLYEYDNEAQRFVAAHHPFTMPTLETLNTFDVNKKDARGRSYDIVLNGYELGGGSVRIIDQQIQRRMFKSINMSDEEANLKFGFLLTAFEYGVPPHCGIALGLDRLMMILVNSEYIRDVVAFPKNNNGVDMMLDAPSNMNDEDLKELGLKIKND" }, "paralog": false, "identity": 0.9910313901345291, "score": 865.0, "evalue": 2.19e-319, "description": "Internal stop codon at 322998, 323970, 324192, 324204. Nonsense mutation around 322998, 323970, 324192, 324204.", "psc": { "uniref90_id": "UniRef90_Q9PQK5", "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.12" ], "uniref50_id": "UniRef50_Q9PQK5", "cog_id": "COG0173", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0003676", "GO:0004812", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006418", "GO:0006422", "GO:0016874" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQK5", "EC:6.1.1.12", "UniRef:UniRef50_Q9PQK5", "COG:COG0173", "COG:J", "GO:0000166", "GO:0003676", "GO:0004812", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006418", "GO:0006422", "GO:0016874" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQK5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK5" ], "product": "Aspartate--tRNA ligase" } }, "id": "GPKLONFDEH_587", "locus": "GCA000006625_00555" }, { "type": "cds", "sequence": "contig_1", "start": 324267, "stop": 324671, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGCCAACTTTAGAAACATTAAATACTTTTGATGTAAATAAAAAAGACGCTAGAGGTCGTAGTTATGATATTGTTTTAAATGGTTATGAATTAGGTGGAGGATCAGTTCGGATTATAGACCAACAAATTCAACGCCGTATGTTTAAATCAATTAATATGAGTGATGAAGAGGCTAATTTAAAATTCGGTTTTTTACTAACTGCATTCGAATATGGTGTTCCGCCACATTGTGGAATTGCTTTGGGTTTAGATCGTTTGATGATGATTTTAGTGAATAGTGAATATATTCGCGATGTTGTTGCATTTCCTAAGAATAACAACGGCGTTGATATGATGTTAGACGCACCATCTAACATGAATGATGAAGATTTAAAAGAATTAGGTTTAAAAATAAAAAATGATTAA", "aa": "MPTLETLNTFDVNKKDARGRSYDIVLNGYELGGGSVRIIDQQIQRRMFKSINMSDEEANLKFGFLLTAFEYGVPPHCGIALGLDRLMMILVNSEYIRDVVAFPKNNNGVDMMLDAPSNMNDEDLKELGLKIKND", "aa_hexdigest": "121088cd2772c7af93041e95067cf6f3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030EC2591", "query_cov": 0.8283582089552238, "subject_cov": 0.9487179487179487, "identity": 0.613, "score": 144.0, "evalue": 3.26e-42, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_A0A358XMQ2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 92, "aa_cov": 0.6865671641791045, "hmm_cov": 1.0, "evalue": 4.221131130908979e-37, "score": 123.39860534667969, "start": 14, "stop": 105 } ], "seq_stats": { "molecular_weight": 15105.176499999989, "isoelectric_point": 4.8733980178833 }, "id": "GPKLONFDEH_588", "locus": "GCA000006625_00556" }, { "type": "cds", "sequence": "contig_1", "start": 324778, "stop": 325260, "strand": "+", "frame": 1, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK4", "UniRef:UniRef90_Q9PQK4" ], "nt": "TTGACTAATAATATTATTGTTGATAAAAATAACAAAAATATTTATCATCAAAACGACAAACAAATTTTTGATTTTACTTTATGTTTATCTGGTCTTTTGTCTAAAACTTTAGGTTTAAATCATAAATCATCTTCGCTTTATTTAACAAATGATGATGAAGTAGCGCATCGTTTTAAGATTAAAGTTTTGCATAATTATTTTAATTTATATAATATTGAAGAATTTTTTACGTTTATGGATAATCAAATTCCTTTAGAAAATGCAAAAAATATTGCACGTTTAAGTTTAATTTATGATTTAAAACAACATGAAAATATTTTAAAAACTATATCGAATGAAGGTTTAGAAAATATTGATTTAGAATTTGCTTTCATTAATTTTTTAAGAGCAAAAATCTCTATTAATTTTGCCTATAGTTATGATATTTCTGAAGTAATTTTATTGTTGCGAACAGGGCTAGATTTACATTTTTTTAGAAAATGA", "aa": "MTNNIIVDKNNKNIYHQNDKQIFDFTLCLSGLLSKTLGLNHKSSSLYLTNDDEVAHRFKIKVLHNYFNLYNIEEFFTFMDNQIPLENAKNIARLSLIYDLKQHENILKTISNEGLENIDLEFAFINFLRAKISINFAYSYDISEVILLLRTGLDLHFFRK", "aa_hexdigest": "4be69a0fa6ce2935e2287eaafcc632ff", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 324664, "stop": 325269, "observations": { "mutation": [ 324748, 325258 ], "stop": [ 324748, 325258 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQK4", "query_cov": 1.0, "subject_cov": 0.7960199004975125, "identity": 0.9940000000000001, "score": 316.0, "evalue": 1.74e-108, "gene_start": 1, "gene_end": 160, "reference_start": 39, "reference_end": 198, "reference_sequence": "MINKKIKINPNITYFKQKISNYHEHLLVWNDLYDKLISLTNNIIVDKNNKNIYHQNDKQIFDFTLCLSGLLSKTLGLNHKSSSLYLTNDDEVAHRFKIKVLHNYFNLYNIEEFFTFMDNQIPLENAKNIARLSLIYDLKQHENILKTISNEGLENIDLEFAFINFLRAKISINFAYSYDISEVILLLRTGLDLHFFRKWAF" }, "paralog": false, "identity": 0.9900497512437811, "score": 397.0, "evalue": 7.85e-143, "description": "Internal stop codon at 324748, 325258. Nonsense mutation around 324748, 325258.", "psc": { "uniref90_id": "UniRef90_Q9PQK4", "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PQK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQK4", "UniRef:UniRef50_Q9PQK4" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQK4" ], "product": "Unique hypothetical" } }, "id": "GPKLONFDEH_589", "locus": "GCA000006625_00557" }, { "type": "cds", "sequence": "contig_1", "start": 325346, "stop": 325471, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGTTGCATATCATAATTTAGATATTAATGACCCTAAAAATGTTCTTTTAGCAACGCAAAAATTCTTTAATCATATTAATGAAATAATTATTGATTATGGTTTAGATAAACGTGATCAATGA", "aa": "MTVAYHNLDINDPKNVLLATQKFFNHINEIIIDYGLDKRDQ", "aa_hexdigest": "715b6b04eb3eaa8b3b20c6ac134d39a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4831.419400000001, "isoelectric_point": 5.326916313171386 }, "id": "GPKLONFDEH_590", "locus": "GCA000006625_00558" }, { "type": "cds", "sequence": "contig_1", "start": 325842, "stop": 326039, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01625.27" ], "nt": "GTGGATCACGATGATGAGTTTATTATTAAAGATTTTTTATTAAAAAAACAAAATCAAACACCAAAAAAAATTATGATTGAAGTTGAAAGATTACGCAATTTTAATATTGCTGAAGAATACCATCAAAAATATTTAGATAAAAATCCTAATAGTTATTGTCATGTTGATTTATCATTATCTAAAAAAGAATTCAGATAA", "aa": "MDHDDEFIIKDFLLKKQNQTPKKIMIEVERLRNFNIAEEYHQKYLDKNPNSYCHVDLSLSKKEFR", "aa_hexdigest": "df7e592796ab79ca0fa5c2d0948b8546", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01625.27", "name": "PMSR", "length": 50, "aa_cov": 0.7384615384615385, "hmm_cov": 1.0, "evalue": 2.8385916126043997e-12, "score": 42.70988082885742, "start": 7, "stop": 54 } ], "seq_stats": { "molecular_weight": 7986.0394, "isoelectric_point": 6.821895408630371 }, "id": "GPKLONFDEH_591", "locus": "GCA000006625_00559" }, { "type": "cds", "sequence": "contig_1", "start": 326075, "stop": 326665, "strand": "-", "frame": 3, "gene": "acrR", "product": "TetR/AcrR family transcriptional regulator", "db_xrefs": [ "COG:COG1309", "COG:K", "RefSeq:WP_006688876.1", "SO:0001217", "UniParc:UPI00000C1C03", "UniRef:UniRef100_A0AAC9T0X1", "UniRef:UniRef50_A0AAC9T0X1", "UniRef:UniRef90_A0AAC9T0X1" ], "nt": "ATGGCAGTAGAAAAAATTAAAATTTCAAAAGTTAAATTAAAAATTGTTTCAGCTTTATTTAATTTGTTGAAAACCAATTCATTTAACAAAATTAAAATTTCACAAATTATTGAGGTTGCAGATGTTTCGCGACCTTCATATTATCGTAATTTTAGTGATAAAATTTCGATTATTAATTATTTTATTAAAAATATTTATAATTGGAAAGTTGTAAGTAAATTAATAAATAATAACCTAAATATCAATTTTGATGAAGTTGGTTTTACTAAATTTTATGAAATTTCATTACAAATTAGTTTAGAATTCAAAGAATATTTTCTAATCTTAGATAAAAATAATTTTAGTGGATTAATTGTTGATTTAACCACTGAAAGTGGTTTTTATGCTATAGGTAATATGTCAAATAATTCAATTGATAAATATTTAATTAATTATTTAACAGGATTTTTTTGTAATATTAGAATGCAATGGTTAAAAAATGGTGCTAAAGAATCAATTCATGATATGGCGGTATTTTTAACTAACGTTTTAAAAGATAATGTTATTAAAAAATTAGAGAAATATCAATCTTGTAATAATATTAATGATTAA", "aa": "MAVEKIKISKVKLKIVSALFNLLKTNSFNKIKISQIIEVADVSRPSYYRNFSDKISIINYFIKNIYNWKVVSKLINNNLNINFDEVGFTKFYEISLQISLEFKEYFLILDKNNFSGLIVDLTTESGFYAIGNMSNNSIDKYLINYLTGFFCNIRMQWLKNGAKESIHDMAVFLTNVLKDNVIKKLEKYQSCNNIND", "aa_hexdigest": "4dca5e4ac4ed570c10b5622f4da132ff", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C03", "ncbi_nrp_id": "WP_006688876.1", "uniref100_id": "UniRef100_A0AAC9T0X1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C03", "RefSeq:WP_006688876.1", "UniRef:UniRef100_A0AAC9T0X1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0X1", "uniref90_id": "UniRef90_A0AAC9T0X1" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0X1", "gene": "AcrR", "product": "TetR/AcrR family transcriptional regulator", "uniref50_id": "UniRef50_A0AAC9T0X1", "cog_id": "COG1309", "cog_category": "K" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0X1" ], "product": "TetR/AcrR family transcriptional regulator" }, "genes": [ "acrR" ], "id": "GPKLONFDEH_592", "locus": "GCA000006625_00560" }, { "type": "cds", "sequence": "contig_1", "start": 327482, "stop": 327802, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAAAAAGTAAGAAAATTCTAGGATTAAGTGTTGCTTCAATTATTGCAGCACTTGCGATTATTGCTCCATCTACTTATTTTGGTTTACAAAAACAAGAAGAGGGTAAAAAAGATATTAATTTAGATAATGTTACGCGAAAAATACCTGGTGAAACTAAAGATGATATCAAAGTTGAAGATTTAAATAATATTAATGATCAAAATACAGATCTTAAACTTGATTTTAGTAAGGTAAGTATTAAACCCCAAACAAAAATTAATTATTTAGCGATTGGTGATTCAATCACAGCTGGTTTTAATTCAGAATTAGGTTGA", "aa": "MDKKSKKILGLSVASIIAALAIIAPSTYFGLQKQEEGKKDINLDNVTRKIPGETKDDIKVEDLNNINDQNTDLKLDFSKVSIKPQTKINYLAIGDSITAGFNSELG", "aa_hexdigest": "4ece85276de056647b6e7f70ef1ccd23", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11580.099199999986, "isoelectric_point": 6.232304573059082 }, "id": "GPKLONFDEH_593", "locus": "GCA000006625_00561" }, { "type": "cds", "sequence": "contig_1", "start": 327959, "stop": 328567, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTGCTGGAAGTGATGAGTATTTTAAAAATCAAGAATATAGATTTTTTGATTTTTCTAAACAAATGGATCAACAAAAAGATAATCCTTTTAAAAACCGTTTATCAAAGTTTTTTAAAAATTTTGGTTATGATTCAAAATTAACACCAAAAAATAATCATGAAGTTAATATTAATGATTTTAATGGTTTAAAAGAGCAAATTAAAAAAGCTAATTTAATGACAATCACCTTAGGTGCTAATGATTTTTTAAGATATTTAGACATTCCAAAACTTGTTAGTTTAACAAATTTGAAAGGTAATGATTTAGAAATAAAAGTTAAAGAGTTCGAAAATAATTTACAAAAGGCATCTTTAGAAATTACAAAAAATCTAAAGAAATTAATCGCTCTTATTCGTACTTTAAATTCTAATGTATCTATTACATTAGTAAGTTATCCATTACCACTATTACGTTTACAAGATGTAATAGATAGTTCATTTAAATTAACAAATAAACGTCGTTTAAGTGATGAAATTATAAATATTCTAAATAGTGCCATTAAAAAAACATATGATTTAAATAATAATATCAACTATATCGATGCTTTTGATGAGAATGATTGA", "aa": "MLGAGSDEYFKNQEYRFFDFSKQMDQQKDNPFKNRLSKFFKNFGYDSKLTPKNNHEVNINDFNGLKEQIKKANLMTITLGANDFLRYLDIPKLVSLTNLKGNDLEIKVKEFENNLQKASLEITKNLKKLIALIRTLNSNVSITLVSYPLPLLRLQDVIDSSFKLTNKRRLSDEIINILNSAIKKTYDLNNNINYIDAFDEND", "aa_hexdigest": "c439ab567e644410200aabe80109295b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23469.51950000003, "isoelectric_point": 9.046155357360838 }, "id": "GPKLONFDEH_594", "locus": "GCA000006625_00562" }, { "type": "cds", "sequence": "contig_1", "start": 328637, "stop": 328729, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCAAGATATTTTTCTAAAAATGTCTTTGGGACAAAATTACGCTCAGCAAAAAACTGCTTCTATTGCAAATGCTATTTATGATGCATGA", "aa": "MAQDIFLKMSLGQNYAQQKTASIANAIYDA", "aa_hexdigest": "7c0c0f0c73fd127b18de5a71ecfdb41b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3305.7340999999988, "isoelectric_point": 5.71319408416748 }, "id": "GPKLONFDEH_595", "locus": "GCA000006625_00563" }, { "type": "cds", "sequence": "contig_1", "start": 329111, "stop": 329941, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTTCTGGAATTCAAAATGATTTAGATAATTTAGATTTAGATAAAAATAATATAATTGGGACACAAAATATTTCTAAAAAAATTTTATATGATGTTTTTAGAAAAAATATTATCAATGATCAGAATTTATATGAATTAATTAAAGGAATGTTTCATTCTCAGCTATTCAATGAAAATAAATCTCAAATTAAATCTTTAATAAAAGCTTTATTAAGTGATTTGGTTAGTTCCCATAAATTATTAGAAATAATTTTTGGTGAATTTGCAACTGATAATTTAAAGAATAATAATGAATTCTTAAAAAATTTACTAATAATCCAAAATAAATTAACTCAAACAAAATCACTAGATAACATTATTAATGTTTTTGTAGATGCTTTGTTTGATAATGATAATGAATATTTATTGCAGGGTCCTTTATCAAATGTTTTAGCAAAAATTGTTAAGACTAATAAAGCATTGTTAACAACTAGAATTGACAATTTATTTAATGATTTATTTAAAGATCAAACAACATTAGATGCAACTTATAACATTCTAATAAGAGTTTTAGGCCAAAATATTCCTGATTTTATAAAAACTCCGCAAGCATCTTTAATAATTAAAAAAATTTTATCAAAAGGTACAAATTTAAAATTAATAAACACTTTAAAATTAAATACGATTGATTTATTAACTGATTCAAAAACATATGCTGGCATATTTGATTCATCAGAAACTTCAATTATCAATCTTATTCAAAAATCCATCGATTTTACTTCAAAATCATTTATTAGCGATGTTTTAGATTTATTAACATCAGATAATGTTGATTTAAACGAATGA", "aa": "MVISGIQNDLDNLDLDKNNIIGTQNISKKILYDVFRKNIINDQNLYELIKGMFHSQLFNENKSQIKSLIKALLSDLVSSHKLLEIIFGEFATDNLKNNNEFLKNLLIIQNKLTQTKSLDNIINVFVDALFDNDNEYLLQGPLSNVLAKIVKTNKALLTTRIDNLFNDLFKDQTTLDATYNILIRVLGQNIPDFIKTPQASLIIKKILSKGTNLKLINTLKLNTIDLLTDSKTYAGIFDSSETSIINLIQKSIDFTSKSFISDVLDLLTSDNVDLNE", "aa_hexdigest": "160f00f805eb32599c76ee4aab5774ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31214.53200000006, "isoelectric_point": 5.329871940612793 }, "id": "GPKLONFDEH_596", "locus": "GCA000006625_00564" }, { "type": "cds", "sequence": "contig_1", "start": 330146, "stop": 330286, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGTTGATGAATTATTAAAAAATCCACAAACTCAAAATTTTGTTACTTCTATAGAAGATTTTATTGCTAATAAATTAGGCGATCTGGTAATTAGTGCAAATAATAGTTTACTAAAATCAAATATGTCTGATATTTGA", "aa": "MSVDELLKNPQTQNFVTSIEDFIANKLGDLVISANNSLLKSNMSDI", "aa_hexdigest": "bd92b876bde28104e852b4c17ed0d215", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5068.686299999999, "isoelectric_point": 4.232879447937012 }, "id": "GPKLONFDEH_597", "locus": "GCA000006625_00565" }, { "type": "cds", "sequence": "contig_1", "start": 330611, "stop": 332605, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAACAATACCTAATTTAACTTTATATAAAGAAATCCGAAAACAAGCTTTTGAATTTTTAACAAACAATATTAATAAAATTTTAGTGGATCCTAAAAACACTTTATTAATATTAAATGATGGGATTAACAAATTATTATCAAACATATTGCCACAATTATCAACCCTGATTGAATTAATTGATAACGATAAAATTAAAGATCAAGATTTTATTGATGTTATTAAAATATTTATAGACAATTTAGATTTTAATAAATTATTTGTCAATATAAAGTCTAATAATAACCAAACAACAGATAGTACTACTTTAAATCAATTTTATTTTAAATTTAAATTAAATTTAGAACCTAAAAAATTAATTTTCGATTTAATAAAAAATCTTTTGAATTCACCAATTTTAAAAGTAAATGATAATAAAAATAATAAAATTAAGATTGAATCTAATAAAGCTAAAATTAAGTCTATTCTAACAACTAGTGTTACAAACATTTTTGAAAATTCTAATCTTATGAATTTTTTTAATAATCAATTATCACAATTATTTAGTTCAATTGATTTATTTAAAAATTTAAATTTATCAAATCAACAACGTTTAAACTTTGCTAAGGCTATTGTTGAATTATTAAATAAAGAAAAATTATTATCAAATTTAATTAATGTTTTAATAACTAACTTAATTGATCACGCTGATGAATATGCTGAAGCAACAGATTTAGCAACAATTTTATCATTAACAATAAAATATAATCAAAATGAATTAAAAAATGCGTTTGATAAATTATTAATAGGAACTTTAAAAAATGATAATCTTCAAGATTTAATTTTACGTATTTTTGTTAAATTAATTAATCCTAACAAGTCTTATGATTCAATTAGTGTAGTTTCAATTAATAAATTGAAAACATTTATATCAAAATTTGCTACAGGAATTAGTAAATTGGATTTATATCAACAATTAAAAAATTCAATATTTACAGTTTTTTCTAACAAAACAAAAATGCAACAATTATTATCAAATGATGTGAGTGTAATAAATAAAACACTACTAGAAGCATTTAACATTGACACACCAGAAAAAGTTTTGAAAATTAGTAGTTTATTAGATATTAATGAAATTGATGCTCGTGATTATGCTGATATTATTGAAATTATTTTAGTTGAAATTGGTTTTAATGTTACAAGTATTCAAAATAATGATGTTAATAATAATTTGAAAACAATTGATAAAAAAACAAATGATTTTGCTTTTCAAATTTTGAAAAAAGCTTTAAATAATGGTGTTAGTGATCAACAATTACAAAAAATTAAAAATGTTGTTAATTATTTATTAGATGATATTGTTAAAATTCATGATTCAAGTAGTTTTTTAAGAATTCAATTAGAACAATTATCACATGTTTTAGTTGATAAAATTACCGCAGTTTTACCTAAATCATTAACAATTAAACATAAATATACAAAGATTTTTTCATCGATTTTAAATAATCAAGATTTTTTACAAAAAGCCAAAACTTTATTATCAACAATTTTAAATGAACTGATTGATCATAAAGATAAATATAAAGACATTAATTCGTTTAGTGAATTAATCTCCGTATTCTTTAAAAATAAAGCTAGCGATCTAAAAACACAATTAAAAGATTTACTAAATACAATTTTAAAAAACCAAACACTAATCACTAATATTGGACAAGTAATAATAGAATCATTTAAACTAGAAAACAAAATAAGTATTCTAGATAGTGATCTTGAACAAAATACGATTTCATTTATTAATAAAATTTTTGCTCACATTACAGAATTGCCAATTTATACTAATTTAGTGGATAATTTTTTTAATTTTTTTAGTGAATATACAAAAAGTTCTGACACAAAAACATTGAATTTTAATAAATTTAAAAGTAGTTTATTCCAAGCTATCATACCTAAAATAAGTGATTTTGCATCTCTTTTAGACTTATTTAAATATAATGAAATTAGCGAACAAGAATGA", "aa": "MPTIPNLTLYKEIRKQAFEFLTNNINKILVDPKNTLLILNDGINKLLSNILPQLSTLIELIDNDKIKDQDFIDVIKIFIDNLDFNKLFVNIKSNNNQTTDSTTLNQFYFKFKLNLEPKKLIFDLIKNLLNSPILKVNDNKNNKIKIESNKAKIKSILTTSVTNIFENSNLMNFFNNQLSQLFSSIDLFKNLNLSNQQRLNFAKAIVELLNKEKLLSNLINVLITNLIDHADEYAEATDLATILSLTIKYNQNELKNAFDKLLIGTLKNDNLQDLILRIFVKLINPNKSYDSISVVSINKLKTFISKFATGISKLDLYQQLKNSIFTVFSNKTKMQQLLSNDVSVINKTLLEAFNIDTPEKVLKISSLLDINEIDARDYADIIEIILVEIGFNVTSIQNNDVNNNLKTIDKKTNDFAFQILKKALNNGVSDQQLQKIKNVVNYLLDDIVKIHDSSSFLRIQLEQLSHVLVDKITAVLPKSLTIKHKYTKIFSSILNNQDFLQKAKTLLSTILNELIDHKDKYKDINSFSELISVFFKNKASDLKTQLKDLLNTILKNQTLITNIGQVIIESFKLENKISILDSDLEQNTISFINKIFAHITELPIYTNLVDNFFNFFSEYTKSSDTKTLNFNKFKSSLFQAIIPKISDFASLLDLFKYNEISEQE", "aa_hexdigest": "7eb16312fd98a2b3bd55ed307b753716", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 76220.1287000004, "isoelectric_point": 8.686615180969238 }, "id": "GPKLONFDEH_598", "locus": "GCA000006625_00566" }, { "type": "cds", "sequence": "contig_1", "start": 332632, "stop": 332910, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACTTAAATTTGATAATAGATTATTAATAAAATTTTTAATATTATTTGATGTTAAAAGCTTATTAAAAGTAAATTCGATAATGTTTTTTGCTTTAGTTAAGGTATTATTATCAAATGTTTTAGCTTTTTGTGATAATGCTTTAAATAAATCTAAAATGATGTTTTCAATTTTATTTAATTCTGGTTTTGTTTTTGAAATTGAAATATTTCTAACAAATAATAAAGAATTAGTAGGCAAAATTTTAGAGTTTATTAAATTATTTATTGGCAAATAA", "aa": "MGLKFDNRLLIKFLILFDVKSLLKVNSIMFFALVKVLLSNVLAFCDNALNKSKMMFSILFNSGFVFEIEIFLTNNKELVGKILEFIKLFIGK", "aa_hexdigest": "556067d337277cd590a582eb3efff016", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10600.842799999991, "isoelectric_point": 9.61205883026123 }, "id": "GPKLONFDEH_599", "locus": "GCA000006625_00567" }, { "type": "cds", "sequence": "contig_1", "start": 333303, "stop": 333713, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAATGATCTTTTAGATAGTTTATTTACAAAATTAAGCGATAAATCTATTTTAAATAAGTTATTAAATGATGATTTTTTATCAATTGGAAGTATTTTAAAAGAAATTTTTAATATAAATCAACCATCACAACTAATAAAAGTTATTGATATTTTTAGTATCAAAGACATTACTGCCCAAGATTATGTAAATGTTTTGAATGTTATCTTTAATGATATTGGTTTTAATAATCAACAAACAAAACAAAATATTACCAATGATCAAAAATACGATATTAATATTTACTTTGAATATTTAAAAGCTTTAGTTGAACATAATTTTAGTGAGTTAACTAAAACAAAATTAAAAGAAATCGTCAAATTATTCGTTAAAGATGTAATGAATGATGACCATCATAACCATTTATGA", "aa": "MYNDLLDSLFTKLSDKSILNKLLNDDFLSIGSILKEIFNINQPSQLIKVIDIFSIKDITAQDYVNVLNVIFNDIGFNNQQTKQNITNDQKYDINIYFEYLKALVEHNFSELTKTKLKEIVKLFVKDVMNDDHHNHL", "aa_hexdigest": "2c6ab22945d6e1a5c9f1aee6a896090a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15927.010699999988, "isoelectric_point": 5.2253450393676735 }, "id": "GPKLONFDEH_600", "locus": "GCA000006625_00568" }, { "type": "cds", "sequence": "contig_1", "start": 333720, "stop": 333965, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATCGTTTATTAGTCATAAATTAAGTGATTTATTAATTAAAAAAATTCAAGTATTAACTCCACAGAAAAATGAATATCAACAATTAGTTAACAATTTATTAAATGGTATTGATTTTAAAAATGTTGTCCAAAATTTAATTGTTGAAGGCTTTGCAAAAATAATTGATCATCAACAACAATTAAAGAACTCACAAAATTTTGGAGATTTAATTCAAAAAGTTATCAATATAGATGATGTATGA", "aa": "MTSFISHKLSDLLIKKIQVLTPQKNEYQQLVNNLLNGIDFKNVVQNLIVEGFAKIIDHQQQLKNSQNFGDLIQKVINIDDV", "aa_hexdigest": "b26e49cd478d6b7a202daf8d599d061b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9304.637499999995, "isoelectric_point": 6.705887031555175 }, "id": "GPKLONFDEH_601", "locus": "GCA000006625_00569" }, { "type": "cds", "sequence": "contig_1", "start": 334908, "stop": 337202, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGCGCGTGTAATATTTAAACAAATTTCTCCACAAAAAAAATATGATCAAATTCAGCAAATAAATAAAGACAAAGTGGTCACTTTTATTAAGAAGCTTGGTGACAATCTGACAAAATTTGATTTATATAATAAATTACTCGATAATTTATTTAGTTCAATTACAAATCAAACAAATTTAACAAAATTATTGAGTGAACAGCAAGATAGTGTAATTAATTTTATTAAAACGATTTTTGATTTTAGTGATCCTAAACAAATTATTTCACTAATTGATGTATTAAAAGTCAACGAGATTACAAGTAATGATTATATTGAAGTTATTAAATCAATTTTAGATGAAATTGATTTTAATCATTTCTTTAATCAAGCTGTCAAAAACAGTAATCTTTCAAATTATCCATTGAATAAACATGAAAATGATAATGATAGTAAATTAGATATAAATAAGTACTATATTTATGTAAAAACATTATTTGCGCATGAAATGGATTCTACATCAAAAGAAAAATTAAAAACAATTATTGATGAACTAACAAAAAAAATTGTTGAAAATAATAATAAGGGCTTGTTGTACAATTTGGGCAATGGATTAGGTGAAAAACTAAGTAATTTAATTATTAATACATTCCCTGGGTTAACTAATTTAAAACAAAACTATAAAACACTAGTACAAAAAGTATTTACTAATCAAAACTTGTTTGAGCAAGTTCAAAATTTGATCACTTATGCAATCAATAGTTTGATTGATCATAAAAATGAATATCAAAATTGTAAAACTTTTGGTGAATTATTAACAACTTACATTAATAAGAATCAAACACAAATTATTGATAAATTAAAAGAAGCAATTAATAAAATAATCAGTGGTTCATCTTCATTAATTGATGAAATTTCAACATCATTTGCTAATACAATTAAAACATATTTTAAATTAGAAAATTTAACAGTAGAAGATATCAATAAACTAAAGTCATTTATTAATTTTGTTATCAAAAATATTTTCAATCTTGAATATGTTAATGATGGTTTAGTTAAATTAATTACGACTTTAAGTTCAAACGCCATAAAAATTGTTGATCAAAACACTAATTTTGTAACTGTTATGCAAAATGATGTTATTTCATATTTAACATCTCCGCAACAAATTGCAAAATTACTTCAAATTGTTGATTTGGATAAAAATGATGACATTTTAAATTTCTTAAAAGTTATTTTATCTCATTTGCCTCAAAACGCATATACTATTTTATTTGGTAATAAAATTAATCTTATAACAAAGAACACAAATCAAAAAACTAATAGAAGTGTAATTCAAAAATATACAATATTAGATTATGTAAAAACTATTTTAAATTCTTCTTACTTAAAAAAACAAGATACAATTGTAAAAATTAAACATATTTTTAAAGATCTTTTAGTAACTATATCTAAATCATCACAATTAAATAATTATATTGGTGAGTTGATTAGTAATACTTTCGCACAAAAACTTTCAAATCTTAGTGGTGTGGATAAACAATATAGTGAATCATTTTTAAAAGAATCATATAAATTTATTATAAGTGATGAAAAATTAATCAAGATTATTGATGAAGTAGTTAATGATTTAGTTGATCATAAAGATGAATATTTAAAAGAAATTACAAACAATAATCAACCATCAATTAATATTATTTTAAATATGTTAATTAAACGAAATGTTGATCAACAAAAAGTTGATTTTGTACCTTTTATAAAACGTTATTTAGTTATTGATTCTTCAACACAATTTATGGCTCAATACCTTTTTAAAACTTTAAAATTAGAAAATACTAATGATCAAGATGTAAAAATTGTTCAAACATTTGTAAAAGAATTAATTAATCAAATTGATCAATTAGATTTTGTAAATTTATTCATTGATAAAATATTTACTTTAATAAAAGAAAATGGACTTGATTTATTTACAAAAGAAGAGGGTATAAAAAAAGTAAATGAAGCTATGAAAACGTTTGGTTTAACTGAAACAACTAATTTATTAAAAATTGCAAAACTTGTCGAACCAAATAAAATTAAACCCCAAACCATAGGTGATCTAATTAATTTAGTATTTGAAAAATCACCATTAGCTGAAGATTTCGAAAGTATAACACAAGATAAAAATCCTTTATATTATGGTTTAAAAAATCTTAAAGAAGATAGTTTAAATGCTGTTTTATTTGCTAGTGGCAAAAAGAAAATAGGAGAAGCAGCAAAAGTTAGTGAAATTAATATGTTAGATGCTATCCAACATTTAATTAATAAATTATGA", "aa": "MLARVIFKQISPQKKYDQIQQINKDKVVTFIKKLGDNLTKFDLYNKLLDNLFSSITNQTNLTKLLSEQQDSVINFIKTIFDFSDPKQIISLIDVLKVNEITSNDYIEVIKSILDEIDFNHFFNQAVKNSNLSNYPLNKHENDNDSKLDINKYYIYVKTLFAHEMDSTSKEKLKTIIDELTKKIVENNNKGLLYNLGNGLGEKLSNLIINTFPGLTNLKQNYKTLVQKVFTNQNLFEQVQNLITYAINSLIDHKNEYQNCKTFGELLTTYINKNQTQIIDKLKEAINKIISGSSSLIDEISTSFANTIKTYFKLENLTVEDINKLKSFINFVIKNIFNLEYVNDGLVKLITTLSSNAIKIVDQNTNFVTVMQNDVISYLTSPQQIAKLLQIVDLDKNDDILNFLKVILSHLPQNAYTILFGNKINLITKNTNQKTNRSVIQKYTILDYVKTILNSSYLKKQDTIVKIKHIFKDLLVTISKSSQLNNYIGELISNTFAQKLSNLSGVDKQYSESFLKESYKFIISDEKLIKIIDEVVNDLVDHKDEYLKEITNNNQPSINIILNMLIKRNVDQQKVDFVPFIKRYLVIDSSTQFMAQYLFKTLKLENTNDQDVKIVQTFVKELINQIDQLDFVNLFIDKIFTLIKENGLDLFTKEEGIKKVNEAMKTFGLTETTNLLKIAKLVEPNKIKPQTIGDLINLVFEKSPLAEDFESITQDKNPLYYGLKNLKEDSLNAVLFASGKKKIGEAAKVSEINMLDAIQHLINKL", "aa_hexdigest": "1944b117a6fc02747af5afa750e418df", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 87871.21900000043, "isoelectric_point": 8.400955772399904 }, "id": "GPKLONFDEH_602", "locus": "GCA000006625_00570" }, { "type": "cds", "sequence": "contig_1", "start": 337804, "stop": 338028, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_231818249.1", "SO:0001217", "UniParc:UPI00031250E2", "UniRef:UniRef100_UPI00031250E2", "UniRef:UniRef50_UPI00031250E2", "UniRef:UniRef90_UPI00031250E2" ], "nt": "ATGCAAATTAATATTAAAAAAATAATAATAAAAATAAGCACTTTTTTAGGGTTAGGAATAATTCCTATTTTAGCAAGTTCTTGTTCTATTATTATTCCTGATTCGATTCCTTTAAAATCAAATGTTTCTTCAATTACTAATGATATTTCAAAATTAGCTAATGATTATATTGATAATCACACAAAATTATTGAGTCTATTAATAAAAATAAAAAAATTAGTTTAA", "aa": "MQINIKKIIIKISTFLGLGIIPILASSCSIIIPDSIPLKSNVSSITNDISKLANDYIDNHTKLLSLLIKIKKLV", "aa_hexdigest": "4b72777c118e4b0e5dfc82561bc0ff33", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00031250E2", "ncbi_nrp_id": "WP_231818249.1", "uniref100_id": "UniRef100_UPI00031250E2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031250E2", "RefSeq:WP_231818249.1", "UniRef:UniRef100_UPI00031250E2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00031250E2", "uniref90_id": "UniRef90_UPI00031250E2" }, "psc": { "uniref90_id": "UniRef90_UPI00031250E2", "uniref50_id": "UniRef50_UPI00031250E2" }, "pscc": { "uniref50_id": "UniRef50_UPI00031250E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031250E2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8100.746499999997, "isoelectric_point": 9.66846866607666 }, "id": "GPKLONFDEH_603", "locus": "GCA000006625_00571" }, { "type": "cds", "sequence": "contig_1", "start": 338037, "stop": 338333, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCAAAAAGCAAAATTTTATGCTGGATATATTTCTCGTCTGCGTTTTATTCAAAAATTAAGATATGAAATTCAAAACGAAAAAAAAACATTGAATAAAATAATTAAAGTTGTGAATGATGCTGATATTAGTACCTTATATGATTTTAAAGATAAAAATCTAACTATTTATACGAAAACATTTTATTTCCATAATTATCAATTTGTCAATGCTAATAAAAAAAATTATTTTATTTCTAAAAAAACTATTAAATCTGTTATTACAGAAAATTTATTAGATATAGGATTTACTTGA", "aa": "MIQKAKFYAGYISRLRFIQKLRYEIQNEKKTLNKIIKVVNDADISTLYDFKDKNLTIYTKTFYFHNYQFVNANKKNYFISKKTIKSVITENLLDIGFT", "aa_hexdigest": "9806fb6bd444878aef03595ac09308a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11764.560399999988, "isoelectric_point": 9.806753349304199 }, "id": "GPKLONFDEH_604", "locus": "GCA000006625_00572" }, { "type": "cds", "sequence": "contig_1", "start": 338385, "stop": 338528, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCAAAAATTTTATTGATTATAAAAAAAATGAAAATAAAATTGCTACTCGTTCGAAAATTTTATATGATATGATTGTTAAAAATGAATCATTAAATATTAATCATAGTGAATATGAATCTTTATTAGTCAATAATGAATGA", "aa": "MTKNFIDYKKNENKIATRSKILYDMIVKNESLNINHSEYESLLVNNE", "aa_hexdigest": "65a55b04e9c122c946b671e2d50c96ae", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5606.300399999998, "isoelectric_point": 6.558503532409667 }, "id": "GPKLONFDEH_605", "locus": "GCA000006625_00573" }, { "type": "cds", "sequence": "contig_1", "start": 338547, "stop": 338642, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATATATTTTTATATCAATATTATAATGAAGGTATAAAAAACGATATTGATTTTTTAAATCATTTTTTATTAAAAGATGCACTAAAATAG", "aa": "MINIFLYQYYNEGIKNDIDFLNHFLLKDALK", "aa_hexdigest": "2fe85b9932384f9cff8ecf428f5a9fe0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3807.3719, "isoelectric_point": 5.410640144348144 }, "id": "GPKLONFDEH_606", "locus": "GCA000006625_00574" }, { "type": "cds", "sequence": "contig_1", "start": 338718, "stop": 339092, "strand": "+", "frame": 3, "gene": "tACO1", "product": "putative transcriptional regulatory protein UPA3_0303", "db_xrefs": [ "COG:COG0217", "COG:KJ", "GO:0003677", "GO:0005829", "GO:0006355", "SO:0001217", "UniRef:UniRef50_Q7NBX7", "UniRef:UniRef90_Q9PQJ6" ], "nt": "ATGAAATGTGCAAAAGAAATTAAAGCTGCCGCAAAAATGGGTGGTCCTAACCCAGAGGCAAATTCGCGTTTAAAAGTTGCTATTGAACGCGCTTTAAATAATAATTTGAGTCGTGATTCAATTGAACGAAACATTAATGGTGCCAGTAAAGATATTGATAATTTAAAAGAATTAACATACGAAGGATATGGACCAAATGGATTAGCAATTATTGTTCGTGCTTTAACTGATAATGAGCAGCGGACAATATCAGCTATTCGTGGTTATTTTTCGAAATTACAAGGTCAGATTGCTAAACCTAATTCTGTTAGTATGTTGTTTAATGAATGTGGACAGTTATTAATTAATAAAGAAACTAAATCTTTAGATGAATGA", "aa": "MKCAKEIKAAAKMGGPNPEANSRLKVAIERALNNNLSRDSIERNINGASKDIDNLKELTYEGYGPNGLAIIVRALTDNEQRTISAIRGYFSKLQGQIAKPNSVSMLFNECGQLLINKETKSLDE", "aa_hexdigest": "283fb7d0e1f1f3725da69da0d28f9788", "start_type": "ATG", "rbs_motif": null, "genes": [ "tACO1" ], "truncated": "both", "pseudogene": { "start": 338652, "stop": 339362, "observations": { "mutation": [ 338715, 339090, 339330 ], "stop": [ 338715, 339090, 339330 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQJ6", "query_cov": 1.0, "subject_cov": 0.5254237288135594, "identity": 0.9440000000000001, "score": 227.0, "evalue": 1.35e-73, "gene_start": 1, "gene_end": 124, "reference_start": 23, "reference_end": 146, "reference_sequence": "MPRKHLIASGINKKQQQQAKIWMKCAKEIKAAAKMGGPNPEANPRLKVAIERALNNNLSRDSIERNINGASKDADNLKELTYEGYGPNGLAIIVRALTDNEQRTISAVRGYFSKLQGQIAKPNSVSMLFNEYGQLLIDKKTKTLDEWFELLVDQDIVDINEDDEIIEILVQPKDFSATKLILENNNANIQSAEIKLIPTDFISLDDHARERLVRFVNACENDDDISWVITNYEEEL" }, "paralog": false, "identity": 0.8940677966101694, "score": 414.0, "evalue": 1.44e-149, "description": "Internal stop codon at 338715, 339090, 339330. Nonsense mutation around 338715, 339090, 339330.", "psc": { "uniref90_id": "UniRef90_Q9PQJ6", "gene": "TACO1", "product": "Probable transcriptional regulatory protein UPA3_0303", "uniref50_id": "UniRef50_Q7NBX7", "cog_id": "COG0217", "cog_category": "KJ", "go_ids": [ "GO:0005829", "GO:0003677", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQJ6", "UniRef:UniRef50_Q7NBX7", "COG:COG0217", "COG:KJ", "GO:0005829", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_Q7NBX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7NBX7" ], "product": "Probable transcriptional regulatory protein MYCGA1330" } }, "id": "GPKLONFDEH_607", "locus": "GCA000006625_00575" }, { "type": "cds", "sequence": "contig_1", "start": 339431, "stop": 340069, "strand": "+", "frame": 2, "gene": null, "product": "Sensor histidine kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AA00", "UniRef:UniRef90_A0A2C9DYP1" ], "nt": "GTGCTTGGATATTCTAGTTTATCAGTTACTATAATAACACCTTTAATTTATAAGATATCATATAATAAACAATACTCATTTATTACTAAAAGTATACGAGAATTAAAAACAATTGATACTAATTTTGTTTCATCTTATTCAGATTTTTATATTCTTCAGAAATCAAGTATATCTAATGACTTGTATAATTATAATTTGATTAACAATTATCATGATTTTGAAAAATTAATAAAAAACGATTTGAATTTTGATAAATATAATAGTGCTTTGATTTATGAAAAAATAAAGGTTAGTTTATTAGAGAAATATAATGAAAATTTTTTCAAAAATAACGATCTGTTATATGTTTCTTATAATTCATTAGACTCAAAATATTTTTTAACTTCAATTTTGAGTTTTGATTACAATAATTTATATTTACCATTTTTAAACACTAAATCGATTTTTAATGATGAGTATGATAATAAAAATTTTTATACAGATTCAGCTCTAAGTACTACTAAAACAGAAGTTAAATTTTTTAGTATTAATAAAAAATATAACATAAAACTTAAACAAATTAAAATTTATAGTATTGAAACGGCAAAGGATTTATTTCTATACAAAAACTATGTTCGAAAGAATAATAAACCTATTTAA", "aa": "MLGYSSLSVTIITPLIYKISYNKQYSFITKSIRELKTIDTNFVSSYSDFYILQKSSISNDLYNYNLINNYHDFEKLIKNDLNFDKYNSALIYEKIKVSLLEKYNENFFKNNDLLYVSYNSLDSKYFLTSILSFDYNNLYLPFLNTKSIFNDEYDNKNFYTDSALSTTKTEVKFFSINKKYNIKLKQIKIYSIETAKDLFLYKNYVRKNNKPI", "aa_hexdigest": "9c6f496459cec5281ee22f02e8c4e188", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2C9DYP1", "query_cov": 1.0, "subject_cov": 0.8617886178861789, "identity": 0.995, "score": 304.0, "evalue": 2.45e-102, "valid": true, "uniref50_id": "UniRef50_A0AAP9AA00" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AA00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AA00" ], "product": "Sensor histidine kinase" }, "genes": [], "id": "GPKLONFDEH_608", "locus": "GCA000006625_00576" }, { "type": "cds", "sequence": "contig_1", "start": 340165, "stop": 340578, "strand": "-", "frame": 1, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "COG:COG3331", "COG:R", "EC:3.1.21.10", "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_Q9PQJ4", "UniRef:UniRef90_Q9PQJ4" ], "nt": "ATGAATAATAAAAACAATGGAATGCATCTTGAAGTATTAATTAATAAATCAATTAGCTTGTTTAATTTACAATATCAAGCTTTTTTTAAAAAAAGGTGTGTTGATATTATTATTAAAAATATTGATAATTCTTATGTTCAAGGAAAAATCAAGCAAAAAAGTGAAACTGATTATTACGGATTCTATAAAGGTGATTATTTTGATTTTGAAGCTAAACAAACAAATAAAAACTCATTTTTAATAAAACAAATACAACCGCACCAACTAGCACATTTGTATTTAATTCATAAAAATTCAGGTTTCTCTTTTTTAATTATTTGTTTCATTAATTACAATCTATATTTTATTATCACTTTTCAACAATTAATTAACTATTATCAAAAAACTAAGCGGAAATCCATTCCCTTTCAATGA", "aa": "MNNKNNGMHLEVLINKSISLFNLQYQAFFKKRCVDIIIKNIDNSYVQGKIKQKSETDYYGFYKGDYFDFEAKQTNKNSFLIKQIQPHQLAHLYLIHKNSGFSFLIICFINYNLYFIITFQQLINYYQKTKRKSIPFQ", "aa_hexdigest": "e996bb61b9d7d290fdc5d37d8bf4c81a", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PQJ4", "query_cov": 1.0, "subject_cov": 0.8058823529411765, "identity": 1.0, "score": 277.0, "evalue": 5.77e-94, "valid": true, "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_Q9PQJ4", "cog_id": "COG3331", "cog_category": "R", "kegg_orthology_id": "K03700", "go_ids": [ "GO:0005737", "GO:0004519", "GO:0000287", "GO:0003676", "GO:0007059", "GO:0006310", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQJ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQJ4" ], "product": "Holliday junction resolvase RecU" }, "genes": [ "recU" ], "id": "GPKLONFDEH_609", "locus": "GCA000006625_00577" }, { "type": "cds", "sequence": "contig_1", "start": 340721, "stop": 341026, "strand": "+", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_P75085", "UniRef:UniRef90_Q9PQJ3" ], "nt": "ATGGCAACAATTATTCAAGCAAAAGATTTACGTGCTGGCCACACTTTTTTATATAAAGGTAATATTTACCAAGTAATAGAAAATTCGTTTAATAAAACAGCTATGCGTGAAGGAATTGTAAAATGTAAAGTTAAAAATTTACGTACTGGTGCAATCACTATTGAAGTTTTAACTGGAGAAAAACTTGAACAAGCAGTGATTGAAAAATCAAAAATGACTTTTAGTTATGATGATGGTAGTGGTTATGTGTTTATGGATAATGATACATATGAACAAATTTCAATTCCTTACAGTCAATTAAGTTGA", "aa": "MATIIQAKDLRAGHTFLYKGNIYQVIENSFNKTAMREGIVKCKVKNLRTGAITIEVLTGEKLEQAVIEKSKMTFSYDDGSGYVFMDNDTYEQISIPYSQLS", "aa_hexdigest": "f573559ed9b68600fb37e17508b9c667", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "efp" ], "truncated": "3-prime", "pseudogene": { "start": 340721, "stop": 341287, "observations": { "mutation": [ 341024, 341183, 341198 ], "stop": [ 341024, 341183, 341198 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQJ3", "query_cov": 1.0, "subject_cov": 0.5372340425531915, "identity": 1.0, "score": 201.0, "evalue": 3.49e-64, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MATIIQAKDLRAGHTFLYKGNIYQVIENSFNKTAMREGIVKCKVKNLRTGAITIEVLTGEKLEQAVIEKSKMTFSYDDGSGYVFMDNDTYEQISIPYSQLSWEKNFIEEGTEVSVMRYDGELMGVSLPDQLVVTIIEAEEAVQGNSVQNATKRAWLESKWEFQVPQFIKSGEKVIINPSNGQYVGRAK" }, "paralog": false, "identity": 0.9840425531914894, "score": 357.0, "evalue": 2.55e-128, "description": "Internal stop codon at 341024, 341183, 341198. Nonsense mutation around 341024, 341183, 341198.", "psc": { "uniref90_id": "UniRef90_Q9PQJ3", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_P75085", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQJ3", "UniRef:UniRef50_P75085", "COG:COG0231", "COG:J", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ] }, "pscc": { "uniref50_id": "UniRef50_P75085", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75085" ], "product": "Elongation factor P" } }, "id": "GPKLONFDEH_610", "locus": "GCA000006625_00578" }, { "type": "cds", "sequence": "contig_1", "start": 341066, "stop": 341185, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTATGATGGTGAATTAATGGGTGTAAGTTTACCAGATCAATTAGTCGTAACAATTATTGAAGCAGAAGAAGCGGTTCAGGGTAATTCAGTTCAAAATGCTACAAAACGTGCATGA", "aa": "MRYDGELMGVSLPDQLVVTIIEAEEAVQGNSVQNATKRA", "aa_hexdigest": "19c4709bcce88799401993e2199a5d16", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4233.7339, "isoelectric_point": 4.3588346481323255 }, "id": "GPKLONFDEH_611", "locus": "GCA000006625_00579" }, { "type": "cds", "sequence": "contig_1", "start": 341290, "stop": 341592, "strand": "+", "frame": 1, "gene": null, "product": "Asp23/Gls24 family envelope stress response protein", "db_xrefs": [ "RefSeq:WP_006688898.1", "SO:0001217", "UniParc:UPI00000C1C0A", "UniRef:UniRef100_A0AAC9T0B7", "UniRef:UniRef50_A0AAC9T0B7", "UniRef:UniRef90_A0AAC9T0B7" ], "nt": "ATGCAGTACATTACCATTAATAATACAAAATCAGGGAAGATTTTAATCTCAAAATCAAATATTAAAAAGTTTATTGAACAAAAATTTAATTTGATTGTTAATAAAAAATTTGTAATTAAGAATATCGATATTGTTCAGTATGATGAATCCCTTGTTGATATATCTATTATTATTTCATTATTTGATATTCATCAAAAAGTTGATCTTGATGAAGTAAGAGATGTACAAAATCATTTAGCTTCTTTTATTTATTCAAATTTAGGCGTTGATACTAAAAGTGTAAATATAGGGATTGATTTATAA", "aa": "MQYITINNTKSGKILISKSNIKKFIEQKFNLIVNKKFVIKNIDIVQYDESLVDISIIISLFDIHQKVDLDEVRDVQNHLASFIYSNLGVDTKSVNIGIDL", "aa_hexdigest": "0c4a2bc01eee682c28658e6d5cdca108", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C0A", "ncbi_nrp_id": "WP_006688898.1", "uniref100_id": "UniRef100_A0AAC9T0B7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C0A", "RefSeq:WP_006688898.1", "UniRef:UniRef100_A0AAC9T0B7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0B7", "uniref90_id": "UniRef90_A0AAC9T0B7" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0B7", "product": "Asp23/Gls24 family envelope stress response protein", "uniref50_id": "UniRef50_A0AAC9T0B7" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0B7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B7" ], "product": "Asp23/Gls24 family envelope stress response protein" }, "genes": [], "id": "GPKLONFDEH_612", "locus": "GCA000006625_00580" }, { "type": "cds", "sequence": "contig_1", "start": 341835, "stop": 341978, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01029.23" ], "nt": "TTGCTAAGCGCATATGGTGAATATCTGGTTTTAAAAACTCCTAAGAAAATTATTATTGATCAAACTTTAATTACAACACATAATTATTCTAACAACGAATCATATAAGTTTATTAATGCAATTTTAGATCAATTATTAAATTAA", "aa": "MLSAYGEYLVLKTPKKIIIDQTLITTHNYSNNESYKFINAILDQLLN", "aa_hexdigest": "360b7de5ee1e396c4caf9374cb335e45", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01029.23", "name": "NusB", "length": 43, "aa_cov": 0.8936170212765957, "hmm_cov": 1.0, "evalue": 2.9517904234095676e-08, "score": 29.758853912353516, "start": 4, "stop": 45 } ], "seq_stats": { "molecular_weight": 5446.230199999999, "isoelectric_point": 6.516783714294434 }, "id": "GPKLONFDEH_613", "locus": "GCA000006625_00581" }, { "type": "cds", "sequence": "contig_1", "start": 341984, "stop": 342244, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20260.4" ], "nt": "ATGCAACGTTACTTTGTTTCTAAAATTAAAGACAAAAAAGTTTATTTTTATGATTCTGATGTTCATCATATCAAGAATGTTATGCGTATGAGATTACATGATAAAGTTATTGTTATTAATAAAACAAAAGCATTTGTTGCTGAGATTATTAATCTTGAACCATTACATGCAACAATTATTAAACCATATACTCTTAATTGTGAATTAGCTACTAAAATTGATTTATTTCAAGCAAGCATTAAACCAAATAATTTTGAGTGA", "aa": "MQRYFVSKIKDKKVYFYDSDVHHIKNVMRMRLHDKVIVINKTKAFVAEIINLEPLHATIIKPYTLNCELATKIDLFQASIKPNNFE", "aa_hexdigest": "2d0714b4f0304a1e911772b5006c56f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20260.4", "name": "PUA_4", "length": 44, "aa_cov": 0.5, "hmm_cov": 1.0, "evalue": 2.295642574599852e-12, "score": 42.473167419433594, "start": 17, "stop": 59 } ], "seq_stats": { "molecular_weight": 10179.905799999988, "isoelectric_point": 9.443796348571777 }, "id": "GPKLONFDEH_614", "locus": "GCA000006625_00582" }, { "type": "cds", "sequence": "contig_1", "start": 342512, "stop": 342688, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04452.19" ], "nt": "TTGACCTATCAAAAAATTGCTTTAGTGATTGGACCAGAAGGTGGTTTTGAACAAATGGAAATTAATTTATTAAAACAATTCGATAATGCTATTTGTATTAGTTTGACAAAAACTATTTTAAGATCAGAAACTGCAAGTCTTTATTTACTTGCAAATCTTGTTAATAAGCTTATTTAG", "aa": "MTYQKIALVIGPEGGFEQMEINLLKQFDNAICISLTKTILRSETASLYLLANLVNKLI", "aa_hexdigest": "25b5a90a02488407201a55a01cbe9199", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04452.19", "name": "Methyltrans_RNA", "length": 51, "aa_cov": 0.8793103448275862, "hmm_cov": 0.9803921568627451, "evalue": 1.9572131599801468e-17, "score": 58.883365631103516, "start": 3, "stop": 53 } ], "seq_stats": { "molecular_weight": 6485.629899999998, "isoelectric_point": 6.068153572082519 }, "id": "GPKLONFDEH_615", "locus": "GCA000006625_00583" }, { "type": "cds", "sequence": "contig_1", "start": 343527, "stop": 343661, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGATCAGGTTGTAGTTAATTCTCTAAATATTGAACAAAAAGTTAAATATCGTGAACAAGCAACACGAATTGTTTTTGAGAAACAAGCTGAACATAATGATCATCTAGTAACTTCTGGAAATTTCTAA", "aa": "MQKDQVVVNSLNIEQKVKYREQATRIVFEKQAEHNDHLVTSGNF", "aa_hexdigest": "c7ad849fee22b7cc233f28e78d61effd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5172.7444000000005, "isoelectric_point": 6.70941104888916 }, "id": "GPKLONFDEH_616", "locus": "GCA000006625_00584" }, { "type": "rRNA", "sequence": "contig_1", "start": 343925, "stop": 345441, "strand": "+", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9895629484670581, "score": 1449.4, "evalue": 1.6e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "ATTTTAAAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAATCGAACGAAGCCTTTTAGGCTTAGTGGTGAACGGGTGAGTAACACGTATCCAATCTACCCTTAAGTTGGGGATAACTAGTCGAAAGATTAGCTAATACCGAATAATAACATCAATATCGCATGAGAAGATGTAGAAAGTCGCTCTTTGTGGCGACGCTTTTGGATGAGGGTGCGACGTATCAGATAGTTGGTGAGGTAATGGCTCACCAAGTCAATGACGCGTAGCTGTACTGAGAGGTAGAACAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGGGCGCAAGCCTTATGAAGCAATGCCGCGTGAACGATGAAGGTCTTATAGATTGTAAAGTTCTTTTATATGGGAAGAAACGCTAAAATAGGAAATGATTTTAGTTTGACTGTACCATTTGAATAAGTATCGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAACGAGCGCAGGCGGGTTTGTAAGTTTGGTATTAAATCTAGATGCTTAACGTCTAGCTGTATCAAAAACTGTAAACCTAGAGTGTAGTAGGGAGTTGGGGAACTCCATGTGGAGCGGTAAAATGCGTAGATATATGGAAGAACACCGGTGGCGAAGGCGCCAACTTGGACTATCACTGACGCTTAGGCTCGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATCATCATTAAATGTCGGCCCGAATGGGTCGGTGTTGTAGCTAACGCATTAAATGATGTGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTTGACAATACACGTAGAACCTTACCTAGGTTTGACATCTATTGCGATGCTATAGAAATATAGTTGAGGTTAACAATATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTTTCGTTAGTTACTTTTCTAGCGATACTGCTACCGCAAGGTAGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATATCTAGGGCTGCAAACGTGCTACAATGGCTAATACAAACTGCTGCAAAATCGTAAGATGAAGCGAAACAGAAAAAGTTAGTCTCAGTTCGGATAGAGGGCTGCAATTCGTCCTCTTGAAGTTGGAATCACTAGTAATCGCGAATCAGACATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGGGAGCTGGTAATATCTAAAACCGCAAAGCTAACCTTTTGGAGGCATGCGTCTAGGGTAGGATCGGTGACTGGAGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "GPKLONFDEH_32", "locus": "GCA000006625_00585" }, { "type": "rRNA", "sequence": "contig_1", "start": 345734, "stop": 348644, "strand": "+", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9952136752136752, "score": 2800.6, "evalue": 4e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TAATAAGTTACTAAGAGCTTATGGTGAATGCCTTGGGACAAACAGGCGATGAAGGACGTGCTAATCTGCGATAAGCAACGGGTAGCTGATAAGAGGCTTTAATCCGTTGATCTCCGAATGAGGAAACTCAATCTAATGAGAATTAGATTACTGTATAAATGAATTCATAGTTTAACAGAGCGATACCTGGTGAAGTGAAACATCTCAGTAACCAGAGGAAAAGAAAACGAAGTGATTCCCTGTGTAGCGGCGAGCGAAAGGGGAGCAGGCCAAACCGAATTTCGATTCGGGGTTGTAGGACTACAATATGGAATAGAGAATGATAGTAGAATTGGTTGGGAAGCCAAATCATAGAGGGTGATAATCCCGTATACGAAATTATTCTTTTACCTAGTAGGATCCTGAGTAGGGCGGGACACGTGAAATCCTGTCTGAATCCACCCAGACCATTGGGTAAGCCTAAATACTAGTTTGTCACCGATAGAGCATAGTACCGTGAGGGAACGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGAACCTGAAACCATAAGCTTACAAGGTGTTAGAGCACATTAATGTGTGATAGCGTGCCTTTTGAAGTATGAGCCAGCGAGTTATTATAGCATGCGAGGTTAAATCGTAGAGATGGAGCCGTAGGGAAACCGAGTCTTAATAGGGCGTTTTAGTATGTTATAATAGACGCGAAACGGGGTGATCTATCCATGGGCAGGTTGAAGGTGAAGTAACATTCACTGGAGGACCGAACCCACTTTCGTTGAAACGACAGGGGATGACCTGTGGATAGTGGTGAAATTCCAATCGAACTCCGTGATAGCTCGTTCTCGTCGAAATATTTTTAGGAATAGCGTTAGATTATTGGGAATATGGGGGTAAAGCACTGAATCTATGATGGCGCCACCTCGGTGTACTGAATAGAATCAAACTCTGAATACCATATTACCTATTCTAGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCGCGAGGGAAACAGCCCAGATCATTAACTAAGGTCCCTAATATATGCTAAGTGGAAAACGATGTTGAGTTTCATAAACAGCAAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGAGACTCTGCGCGGAAGATGTAACGGGGCTAAGCATATAACCGAAGTTATGGGTTACATATTATATATGTAGCGGTAGACGAGTGTTGTATATGGGGCGAAGGTAGACTGTGAAGACTACTGGACTTTATACAAGTAAGAATGCTGGCGTGAGTAACGAATGAGAGTGAGAATCTCTCAAACCGATTGACTAAGGGTTCCTGGGCAAGGGTCGTCCTCCCAGGGTAAGTCGGATCCTAAGGCGAGGCTGAAAAGCGTAGTCGATGGAAAACAGGTTAATATTCCTGTACCTACAAATTGTGTGATGGAGTGACGGAGAAGGTTATTATGTGCCGGTTATTGGATTCCGGTTTAAATAACAAGGTTAGTAAGTTGGCAAATCCGCTTACTATTAGGACCAAGTTATGAATACGAGCGACCCCTTCGGGCAGTAGCGAAGACATATACATCATGCTTCCAAGAAAAGCTTCTAGCGTTAAACAATTGGTAGTCCGTACCGAGAACGAACACACGTGGTCAAGGAGAATATCCTAAGGTTAGCGAGTTAACTACAGTTAAGGAACTCTGCAAATTAACCCCGTACGTTAGCAATAAGGGGTGCTCGCTGTAAAAGGTGAGCCGCAGTGAATAGCGAGGGGGGACTGTTTAACAAAAACACAGCTCTATGCTAAGTCGTAAGACGATGTATATGGGGTGACACCTGCCCAATGCTGTAAGGTTAAAGAAGAATGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAATGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCAACTGTAGACTCGGTGAAATCCTGGTGAGGGTGAAGACGCCCTCTTGGCGTGATTGGACGGAAAGACCCCATGAAGCTTTACTGTAGCTTAATATTGGGAAATTTTATTACTTGTAGAGCATAGGTAGGAGACTGTGAAGTATACTCGCTAGGGTATATGGAGTCAACGTTGGAATACTACCCTTGTGATAAGATTTCTCTAACCTGCAGCCATGAATCTGGCTGGGGGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCCAAAAGGTAACGGAGGCGCGCAACGGTACCCTCAGCACGGTTGGAAATCGTGTATAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAAGTCGAACAGGTAGGAAACTAGGTCATAGTGATCCGGTGGCTCAGAATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCTGGGGATAACAGGCTGATAGTGCCCAAGAGTTCATATCGACGGCACTGTTTGGCACCTCGATGTCGACTCATCTCATCCTGGAGCTGAAGCAGGTTCCAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTGTCATGCCCGTAGGAAGATTGAGAAGAGCTGTTCCTAGTACGAGAGGACCGGAATGGACACACCTCTTGTGATCCTGTTGTCGCGCCAGCGGCACTGCAGGGTAGCAACGTGTGGAATAGAGAAACGCTGAAAGCATCTAAGTGTGAAACTAACTTCAAGATTAATCTTCCCTTTCTTAAATGAAGTAAGAATCGTTAAAGACTATAACGTTGATAGGTCGGATGTGTACGCACGGTGACGTGTTTAGCTAACCGATACTAATAATTCGAGGACTTATATAA", "id": "GPKLONFDEH_37", "locus": "GCA000006625_00586" }, { "type": "rRNA", "sequence": "contig_1", "start": 348709, "stop": 348812, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8739495798319328, "score": 82.2, "evalue": 1.1e-13, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TGTAGTGATTATATCAGAGTGGAAATACCTGTTCCCATCCCGAACACAGAAGTCAAGCACTCTAGAGCCGAAAATAGCGCAAGTAAAATAGGTCATCGCTACGC", "id": "GPKLONFDEH_35", "locus": "GCA000006625_00587" }, { "type": "cds", "sequence": "contig_1", "start": 349251, "stop": 349394, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01272.25" ], "nt": "ATGGATTCAGATCCTTTTGCAAACAAAATTTCAATAGACACACCACTTGGAAAAGCTCTTGTAAAACAAAAAAAAGGTACTGAAGTTACAGTTCATACTTTAGCATCACCTTATAAAGTAAAAATTATAAAAATTATTGATTAA", "aa": "MDSDPFANKISIDTPLGKALVKQKKGTEVTVHTLASPYKVKIIKIID", "aa_hexdigest": "6de173cc12348190b0bd6014e9d35bb1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01272.25", "name": "GreA_GreB", "length": 44, "aa_cov": 0.9361702127659575, "hmm_cov": 1.0, "evalue": 8.81271244505027e-15, "score": 50.11517333984375, "start": 2, "stop": 45 } ], "seq_stats": { "molecular_weight": 5153.0432, "isoelectric_point": 9.457012367248538 }, "id": "GPKLONFDEH_617", "locus": "GCA000006625_00588" }, { "type": "tRNA", "sequence": "contig_1", "start": 349474, "stop": 349557, "strand": "+", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 67.4, "nt": "GGACAGATAGCGAAGTGGCCAAACGCATCCGGCTGTAACCCGGCTCCGAGAGGTTCGTAGGTTCGAATCCTACTCTGTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 349508, 349510 ], "id": "GPKLONFDEH_5", "locus": "GCA000006625_00589" }, { "type": "tRNA", "sequence": "contig_1", "start": 349563, "stop": 349637, "strand": "+", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 65.9, "nt": "TGGCCTGTAGCCAAGAGGTAAGGCAGAAGATTTTGATTCTTCCATGCGTAGGTTCGATCCCTACCAGGCCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 349595, 349597 ], "id": "GPKLONFDEH_6", "locus": "GCA000006625_00590" }, { "type": "tRNA", "sequence": "contig_1", "start": 349705, "stop": 349779, "strand": "+", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 75.6, "nt": "GCCTCATTAGCTCAGTGGTAGAGCATTTCACTTTTAATGAAGGGGTCAAAGGTTCGAAACCTTTATGGGGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 349730, 349732 ], "id": "GPKLONFDEH_7", "locus": "GCA000006625_00591" }, { "type": "tRNA", "sequence": "contig_1", "start": 349840, "stop": 349925, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 74.9, "nt": "GCTGGAGTGGCGGAATGGTAGACGCACTGGACTTAAAATCCAGTGACTTCACGGTCGTGAGGGTTCAAGTCCCTCTTCCAGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 349873, 349875 ], "id": "GPKLONFDEH_8", "locus": "GCA000006625_00592" }, { "type": "tRNA", "sequence": "contig_1", "start": 349929, "stop": 350002, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 79.9, "nt": "GCGGGTGTAGTTCAATGGTAGAACTATAGCCTTCCAAGCTATTAGTGTGGGTTCGATTCCCATCACCCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 349961, 349963 ], "id": "GPKLONFDEH_9", "locus": "GCA000006625_00593" }, { "type": "cds", "sequence": "contig_1", "start": 350065, "stop": 350724, "strand": "+", "frame": 1, "gene": "trmN6", "product": "tRNA1(Val) (Adenine(37)-N6)-methyltransferase", "db_xrefs": [ "COG:COG4123", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXG4", "UniRef:UniRef90_A0AAC9T2A9" ], "nt": "ATGCAAAAAAAATACATTAAAAATACTTTAGGCTTTGATACAAATTTATTTATATATCAAGATAAAAAAATGTTCAATTATTCAATTGATACTATTCTGTTAGCAAATTTTATAAGCTTATCTTCTAAGACGAAAAAAATTTTAGAAATAGGAACAAATAATGCTGCATTGAGTATTTTTTTAGCTTTGCGCAAAGAGGATATAAAAATTGATGCGATTGAAATTCAAATAGAAGCAATTAACCTTGCATTATTAAATGTTAGTCAAAATCATTTTGAAAAACAAATTAATATTATACATGCTGATTTTAATCAATATTGGAAAGATTTTGATAAAAGCGAAAATAATAAATATGATGCAATAATCTGTAACCCCCCTTTTTATAAACAAGATAAAATTATTCCATCAACTAAAAATCCATTAAAAACTTTAGCTTTATATGAAATCACGCTTAATTTTGAACAAATTATGCAAGGGTGTGCTAAGATAATTAAACAAAAAGGAAATTTAGCAATGGTTATTCCTACAACGCGTTTAGTTGATCTTTTAGAAATTATGCGTAAATATCAATTTGAACCAAAACGAATTAAAATGATTTATCCACGAATCTATGAACAATCAAATTTAGTTTTAATAGAAGCGCGTTACAAAAGTGGTTGA", "aa": "MQKKYIKNTLGFDTNLFIYQDKKMFNYSIDTILLANFISLSSKTKKILEIGTNNAALSIFLALRKEDIKIDAIEIQIEAINLALLNVSQNHFEKQINIIHADFNQYWKDFDKSENNKYDAIICNPPFYKQDKIIPSTKNPLKTLALYEITLNFEQIMQGCAKIIKQKGNLAMVIPTTRLVDLLEIMRKYQFEPKRIKMIYPRIYEQSNLVLIEARYKSG", "aa_hexdigest": "1bff32caaceb9e5678dc71c5c71ffb15", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAC9T2A9", "query_cov": 1.0, "subject_cov": 0.8588235294117647, "identity": 1.0, "score": 431.0, "evalue": 2.47e-152, "valid": true, "gene": "TrmN6", "product": "tRNA1(Val) (Adenine(37)-N6)-methyltransferase", "uniref50_id": "UniRef50_A0A448ZXG4", "cog_id": "COG4123", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4" ], "product": "DNA methylase" }, "genes": [ "trmN6" ], "id": "GPKLONFDEH_618", "locus": "GCA000006625_00594" }, { "type": "cds", "sequence": "contig_1", "start": 350876, "stop": 351796, "strand": "+", "frame": 2, "gene": "nfo", "product": "deoxyribonuclease IV", "db_xrefs": [ "EC:3.1.21.2", "GO:0003677", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833", "SO:0001217", "UniParc:UPI000165F8B3", "UniRef:UniRef100_Q9PQI5", "UniRef:UniRef50_Q9PQI5", "UniRef:UniRef90_Q9PQI5" ], "nt": "ATGAATAATATGGATAAATATAATTTAATTATAGGTAGTCATGTAAGTTTAAAAGCTAAAGATTTCTTTTATGGTAGTGTAAAAGAAGCATTATCTTATGGTTCTAACACGTTTATGGTTTATACTGGAGCTCCACAAAATACAAAACGTCAACCGATTAAATCATTTAAAATTGAAGAAGCACATAATTTGTTAAAAAAACATAATATTAATTTGGATGATTTAATTGTTCACGCTCCATATATTATCAACCCTTGTTCTTCTAAAAAGAATGTTCGTGAACTTGCTAAAGAATTTTTAATTCAGGAAATCCAACGAACAGAATCTATGGGGATCACTAAACTTGTTTTACATCCAGGATCACGTTTAGAACAAAATGAAGATATAGCTTTAGAACAAGTTTATACAATGTTAAATGATATTTTTTCAACTATTAATACTAATGTAATTGTTTGCTTAGAGACCATGGCTGGTAAAGGTTCTGAAATTGGGGTAAATATAAAACAATTAAAAACCATTATAGACAATGTTCATTCTAAAAAAAATATTGGCGTTTGTTTAGATACTTGCCATATGAATGATAGCGGTTTGATTTTAGATTATTATAATTTTAATCAATATTTAAAAGAATTTGATGCACAAATTGGTATTAATTATATTAAAGTATTACATATTAACGATTCTAAAAACCCTTGTGGTGCAAACAAAGACCGCCACGAAAATTTAGGATATGGAACGATAGGTTTTGCAAATTTAATTAATATTATTTACCATCCCTTATTAAATAACATCCCTAAAATTTTAGAAACGCCTTGGTTTAATGTTAATGATGAATTAATTCCTTTATATAAGCATGAAATAAAAATGATTCGCGACTGCAAGTGGTATGACATTAAAAACAAATTATTATCGAAGAAATAA", "aa": "MNNMDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHNLLKKHNINLDDLIVHAPYIINPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKGSEIGVNIKQLKTIIDNVHSKKNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHENLGYGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLLSKK", "aa_hexdigest": "e93d1610e41203a65ca6c7570ebc14ed", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000165F8B3", "uniref100_id": "UniRef100_Q9PQI5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8B3", "UniRef:UniRef100_Q9PQI5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQI5", "uniref90_id": "UniRef90_Q9PQI5" }, "psc": { "uniref90_id": "UniRef90_Q9PQI5", "gene": "nfo", "product": "deoxyribonuclease IV", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_Q9PQI5", "go_ids": [ "GO:0003677", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQI5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQI5" ], "product": "Probable endonuclease 4" }, "genes": [ "nfo" ], "id": "GPKLONFDEH_619", "locus": "GCA000006625_00595" }, { "type": "cds", "sequence": "contig_1", "start": 352030, "stop": 352245, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTATTATTGAAAATAGATTAAAATCAACAAGACAAACCATTTTTATTTCTAATTTTTCAATTGAACAATTAATTAATTCAACAAAAAATACAACAAATATTGAAGAACAAAAAACTAAATTACGCTTATTTAATCGTATCGAATATCTCACATATGGTAATATTTTTAAAATAACAGGGCCAAGTGTTTTTAAAGTTGTAAATAATTCATAA", "aa": "MPIIENRLKSTRQTIFISNFSIEQLINSTKNTTNIEEQKTKLRLFNRIEYLTYGNIFKITGPSVFKVVNNS", "aa_hexdigest": "50c44174bcf7dd58591b2e4841215fb3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8296.469299999995, "isoelectric_point": 9.991777610778808 }, "id": "GPKLONFDEH_620", "locus": "GCA000006625_00596" }, { "type": "cds", "sequence": "contig_1", "start": 352264, "stop": 352830, "strand": "-", "frame": 1, "gene": "dnaC", "product": "ATP-binding protein", "db_xrefs": [ "COG:COG1484", "COG:L", "SO:0001217", "UniRef:UniRef50_A0AA86Z0S2", "UniRef:UniRef90_A0AAC9T3C2" ], "nt": "TTGAATAATTTAAACAATCAAAATGATAGTTATTACATTTATCAATTAAATCGTAATGAATATAATCACTTATGTTTAGTAGTAGTTAAAAAAGAAAATAAAGTAGATAAAATTAAAAGAAATTATATTTTAAATACGATCAAAAATATGGATTATAATATCTCATTAGCAGATGATAGTCAAATATTTTCTAAAAAAACTGAAATATTAGATGCTAATTTATTAGCAGAACGAAATAAATTAATTAATTTCTTTTTAGAAGAAGCACGAAAAAATAAAAAAAATACGTTAATTAAAAATAATAATCAACAACCAAAATCTGCTTTTATTTATGGTGATTTTAGTGTAGGCAAATCAATAATTACTCAAGCTTATACCAATACTATTTCATTAAAATATAACTTAAAGATAGCTTATATTACTTTAAATGATCTATTTAAAAATGTAATCCAATTTTTTAACTACAAAGATACATCAGATTTAGTAATTAATGAATTAGTTAATCTACTTTCAAATGTTGATGTTTTAGTAATTGATGATTTTTCTAGCGTTAATTTAAATTACTGA", "aa": "MNNLNNQNDSYYIYQLNRNEYNHLCLVVVKKENKVDKIKRNYILNTIKNMDYNISLADDSQIFSKKTEILDANLLAERNKLINFFLEEARKNKKNTLIKNNNQQPKSAFIYGDFSVGKSIITQAYTNTISLKYNLKIAYITLNDLFKNVIQFFNYKDTSDLVINELVNLLSNVDVLVIDDFSSVNLNY", "aa_hexdigest": "986bc07de6293bfd76cb2985e122cd41", "start_type": "TTG", "rbs_motif": null, "genes": [ "dnaC" ], "truncated": "both", "pseudogene": { "start": 352030, "stop": 352980, "observations": { "mutation": [ 352266, 352860 ], "stop": [ 352266, 352860 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3C2", "query_cov": 1.0, "subject_cov": 0.5949367088607594, "identity": 0.995, "score": 356.0, "evalue": 2.98e-122, "gene_start": 1, "gene_end": 188, "reference_start": 51, "reference_end": 238, "reference_sequence": "MNNNQVDDELDLENFNYQKALEIPNIKAINLTEEEFNLYFWDIVEVYRSYLNNLNNQNDSYYIYQLNRNEYNHLCLVVVKKENKVDKIKRNYILNTIKNMDYNISLADDSQIFSKKTEILDANLLAERNKLINFFLEEARKNKKNTLIKNNNQQPKSAFIYGDFSVGKSIITQAYTNTISLKYNLKIAYITLNDLFKNVIQFFNYKDTSDLVINELVNLLSNVDVLVIDDFSSVNLNYWSISTILMPIIENRLKSTRQTIFISNFSIEQLINSTKNTTNIEEQKTKLRLFNRIEYLTYGNIFKITGPSVFKVVNNS" }, "paralog": false, "identity": 0.9936708860759493, "score": 614.0, "evalue": 2.12e-226, "description": "Internal stop codon at 352266, 352860. Nonsense mutation around 352266, 352860.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3C2", "gene": "DnaC", "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0AA86Z0S2", "cog_id": "COG1484", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3C2", "UniRef:UniRef50_A0AA86Z0S2", "COG:COG1484", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_A0AA86Z0S2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA86Z0S2" ], "product": "Chromosomal replication initiator protein DnaA ATPAse domain-containing protein" } }, "id": "GPKLONFDEH_621", "locus": "GCA000006625_00597" }, { "type": "cds", "sequence": "contig_1", "start": 352858, "stop": 352980, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAATAATCAAGTTGATGATGAATTAGATTTAGAGAATTTTAATTATCAAAAAGCATTAGAAATTCCGAATATTAAAGCAATTAATTTAACAGAAGAAGAATTTAATTTATACTTTTGA", "aa": "MNNNQVDDELDLENFNYQKALEIPNIKAINLTEEEFNLYF", "aa_hexdigest": "f562db6573753fa41b5c8bcf04baee46", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4807.215799999999, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_622", "locus": "GCA000006625_00598" }, { "type": "cds", "sequence": "contig_1", "start": 352983, "stop": 353534, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATTTAATCAAAAATAATTTAATTAATAATATTGATTTTTTAACAATTGATTTAAATAAATTAGAATTAACAATTTTTTCAAATATGGAATTCGAAAAAAATAAAACAAAATTTCAATATTACAATATTGAACGTGATTTTAATATTTTTAATAAAAACAGTAATTTAGATCGATTCAAAAATGTAATTAATGATTATAATAATTTTAAACCTTCAGATTTTTTAAAACATATTCGTAAAGAAAAATTAAGTTTAAAAGATAAAGAGGCTATCAATTTATTAACGTATGACAAATCATTAAGTGATCCAATTGTTAATGTTATCTTAGATTTTGTTTTATTTAAAAATTTAGGGCGTTTAAATGTAAAATATTTAAAACGTATTAAAGAAACAGTACTGGGAATCAATATTAAAGATGAGTATGAAATGATTAAATTTTTTCAAAATAAAAAGAATGATCATAAAGAATTAATTTGTGAAGATAGTGATAATGCTAATACTTTCAAAAATACTAATTTTATTGAAGTAAAATTTGGAGATTTATAA", "aa": "MLNLIKNNLINNIDFLTIDLNKLELTIFSNMEFEKNKTKFQYYNIERDFNIFNKNSNLDRFKNVINDYNNFKPSDFLKHIRKEKLSLKDKEAINLLTYDKSLSDPIVNVILDFVLFKNLGRLNVKYLKRIKETVLGINIKDEYEMIKFFQNKKNDHKELICEDSDNANTFKNTNFIEVKFGDL", "aa_hexdigest": "c600f5afd5a08cbc6175c9d3695269f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21879.92050000001, "isoelectric_point": 8.75205059051514 }, "id": "GPKLONFDEH_623", "locus": "GCA000006625_00599" }, { "type": "cds", "sequence": "contig_1", "start": 354021, "stop": 354128, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATAGTAATTAAGAAAAATTGTAATTTTGTTAAAAATGATGACCATTTATATTCTTTTTATACACCACTAATTGGTATGCAAGCAGTAGCTTTATATTCTTGA", "aa": "MDIVIKKNCNFVKNDDHLYSFYTPLIGMQAVALYS", "aa_hexdigest": "038fd05ed8d7fdb8e46125d83ad2e941", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4052.6922000000004, "isoelectric_point": 6.498140525817871 }, "id": "GPKLONFDEH_624", "locus": "GCA000006625_00600" }, { "type": "cds", "sequence": "contig_1", "start": 354570, "stop": 355025, "strand": "+", "frame": 3, "gene": null, "product": "Sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6I6", "UniRef:UniRef90_A0AAC9X6I6" ], "nt": "TTGAATTTTAAATTTTATACTTTAGAGATTGAAGCAAATGATTTAAAAACTATTATTTATGAAATATTTTTAAAAATATTTCAAGTAACTAGAATTAAAAGTTTAAATCAATTTATAGGATTTGTTAAACGGCAAATTTATTTTTATTTAGTATATTTATTACGTAAATCTTTATCTAATAAAAATTTAGTTAATCATTTAAATAATAATAATTTTGAAAATTATGAATTAATTAGTACCAATTCAGATGAAGTTGGTGCTGAATTAAACAACAGATTTTTAATTTATGAATTTTTAAGAAGTATTCAGAAAGAAAATTTATTTTTATATCATTTTACTATTTTAATTATTAAAGGATATAATACAAAGGAAATTAGTGAAATTTTAAAAATTAATTCTGCAAAAATTTATTATTATAAAGCACTTTTAAAAAAATATATAAAAAAATTATTATAA", "aa": "MNFKFYTLEIEANDLKTIIYEIFLKIFQVTRIKSLNQFIGFVKRQIYFYLVYLLRKSLSNKNLVNHLNNNNFENYELISTNSDEVGAELNNRFLIYEFLRSIQKENLFLYHFTILIIKGYNTKEISEILKINSAKIYYYKALLKKYIKKLL", "aa_hexdigest": "93fedae9361fa4c7765900a45c69163b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAC9X6I6", "query_cov": 1.0, "subject_cov": 0.8118279569892473, "identity": 0.993, "score": 280.0, "evalue": 1.52e-94, "valid": true, "product": "Sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0AAC9X6I6" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6I6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6I6" ], "product": "Sigma-70 family RNA polymerase sigma factor" }, "genes": [], "id": "GPKLONFDEH_625", "locus": "GCA000006625_00601" }, { "type": "cds", "sequence": "contig_1", "start": 355093, "stop": 355347, "strand": "-", "frame": 1, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0070181", "KEGG:K02968", "RefSeq:WP_006688746.1", "SO:0001217", "UniParc:UPI0000134E5E", "UniRef:UniRef100_Q9PQI1", "UniRef:UniRef50_Q9PQI1", "UniRef:UniRef90_Q9PQI1" ], "nt": "ATGGCAAATATTGTTTCAAATGAAAAAACATACAGACATACGCAAAAAGTACGTAAGGAAAATCATGCTAAAATGTCTAAATTAAGAACAATTGTTAAAAAAACAAGAAGTTCAAATGAACAAGCTCAACTTAATGAAGCTTACAAAGTCATTGATACAACAGCTTCAAAAGGTGTTATTCACAAAAATAAAGCAAATCGTTTAAAATCAAGAACAGCAAAAGCTTTTAAAACAAACTTAGAAGTCACAGCATAA", "aa": "MANIVSNEKTYRHTQKVRKENHAKMSKLRTIVKKTRSSNEQAQLNEAYKVIDTTASKGVIHKNKANRLKSRTAKAFKTNLEVTA", "aa_hexdigest": "8a65af6ef039e1d1e11a57ddf0b133fa", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000134E5E", "ncbi_nrp_id": "WP_006688746.1", "uniref100_id": "UniRef100_Q9PQI1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E5E", "RefSeq:WP_006688746.1", "UniRef:UniRef100_Q9PQI1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQI1", "uniref90_id": "UniRef90_Q9PQI1" }, "psc": { "uniref90_id": "UniRef90_Q9PQI1", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_Q9PQI1", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQI1" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "GPKLONFDEH_626", "locus": "GCA000006625_00602" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 355530, "stop": 355892, "strand": "+", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 243.3, "evalue": 2.3e-26, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "GTAAAAAGCTAATTGCTGCATTTTTTAAAAAATGTAGAGGAAAGTCCGTGCTCACACTAACTGTGATGTTAGTAGTGTTTGTGTATGTATAATTAATAAGGCATAGCATAGGCATATGACGGCAAATTTTTAAGCTAAGTGATTTAAATTATATGTTTAAAAATTTATAAAAGTGCCACAGAGACGATTAATCAAGAAATTGATGAGATGAAACGTTGGTAAACCCCACAAGTGAGAAACCTAAATTTCGGTAAGGGAATCTAATTTAGTAAATTAAGAAATTAAATTAGGATAGAATTGTTTACATTCTATAGATAAATAATTAGCGCTACATTTTTGTAGAACAGAACACGGCTTATTTTT", "id": "GPKLONFDEH_38", "locus": "GCA000006625_00603" }, { "type": "cds", "sequence": "contig_1", "start": 355954, "stop": 356376, "strand": "-", "frame": 1, "gene": "rimI", "product": "N-acetyltransferase", "db_xrefs": [ "COG:COG0456", "COG:J", "SO:0001217", "UniRef:UniRef50_A0AAC9X727", "UniRef:UniRef90_A0AAC9X727" ], "nt": "ATGTTAAAAAACCTCTTAATTAGACCTGCTGATCAAAAAGATTTGAATGATATTGCTATTTTTGAAAATAGATTTTTCTTAAATGATTGTTATTCGATGAGAAATTTAGAAGAAATTTTTCATAATTCAAATTATCAAATAATAATTGTTGAATATGAGCAAAAAAACATTGCTTATTTAATTTATATGAAGAGTGTTGATTTTAATGAAATTTTAAAAATTGGTGTTGATGAAAATTATCGTAATTTAAAAATTGGTAGTTTATTAATTGATTTATTAAAACAAAAAAAGCAAAACATATTATTAGAAGTAAGCGATCTTAATACAAATGCATTAAATTTTTATTTAAAGCATGGTTTTATAAAACAAAACATTCGCAAAAGATATTATGCCAATGGTTCTGATGCTATTTTAATGATTTGA", "aa": "MLKNLLIRPADQKDLNDIAIFENRFFLNDCYSMRNLEEIFHNSNYQIIIVEYEQKNIAYLIYMKSVDFNEILKIGVDENYRNLKIGSLLIDLLKQKKQNILLEVSDLNTNALNFYLKHGFIKQNIRKRYYANGSDAILMI", "aa_hexdigest": "1bbc0d30d0733eb8c18d23fa86f72ae7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAC9X727", "query_cov": 1.0, "subject_cov": 0.958904109589041, "identity": 1.0, "score": 278.0, "evalue": 1.42e-94, "valid": true, "gene": "RimI", "product": "N-acetyltransferase", "uniref50_id": "UniRef50_A0AAC9X727", "cog_id": "COG0456", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X727", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X727" ], "product": "N-acetyltransferase" }, "genes": [ "rimI" ], "id": "GPKLONFDEH_627", "locus": "GCA000006625_00604" }, { "type": "cds", "sequence": "contig_1", "start": 356354, "stop": 356929, "strand": "-", "frame": 3, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "COG:COG1214", "COG:J", "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_006689124.1", "SO:0001217", "UniParc:UPI00000C1C14", "UniRef:UniRef100_A0AAC9X7X4", "UniRef:UniRef50_A0AAC9X7X4", "UniRef:UniRef90_A0AAC9X7X4" ], "nt": "ATGAATAGTACATACCAATTGTTTATTGATGTTACATCAAGAAAATGTATTTTAGCAATTTATAAAAACTTTAAAATCCATACTAGCATTATTGTTGATACAAACAATAATCTTACAGATATTATTGTTGAACACCTTAATAATTTATTAAAAATCACTAACTTGCAATATCAAGATCTAGAAGCAATATATTTAAATACTGGTCCAGGATCATTTACTGGAATACGTGTTGGTGCTATTGTTGCTAAAACGATTTGTACAATTTTTAATAAAATTAAACTTTTTATTAATGATAGTTTAAATATTATAGCCAATGGTAAGAATGATGTTTTTGTTTATTTAGATGCTAAAGGTAATAAATCGTATGTAATAACTATTACTAATAATATTCAAAACGAATATAAAATTATTACTAATAAAGAATTACAAAATGAATTAAAAAATACATCATTAACAATAATTGATGCAAATCAAATTGACTATCATGATCTCATATATAACTTAAAATTTGATAATTTTAAGTTAACTAATATCTTAGATTTTAGATTAAATTATGTTAAAAAACCTCTTAATTAG", "aa": "MNSTYQLFIDVTSRKCILAIYKNFKIHTSIIVDTNNNLTDIIVEHLNNLLKITNLQYQDLEAIYLNTGPGSFTGIRVGAIVAKTICTIFNKIKLFINDSLNIIANGKNDVFVYLDAKGNKSYVITITNNIQNEYKIITNKELQNELKNTSLTIIDANQIDYHDLIYNLKFDNFKLTNILDFRLNYVKKPLN", "aa_hexdigest": "34fecd2a37d32594dddd2b7bb90ca4db", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C14", "ncbi_nrp_id": "WP_006689124.1", "uniref100_id": "UniRef100_A0AAC9X7X4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C14", "RefSeq:WP_006689124.1", "UniRef:UniRef100_A0AAC9X7X4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X7X4", "uniref90_id": "UniRef90_A0AAC9X7X4" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X7X4", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_A0AAC9X7X4", "cog_id": "COG1214", "cog_category": "J", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X7X4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X7X4" ], "product": "tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB" }, "genes": [ "tsaB" ], "id": "GPKLONFDEH_628", "locus": "GCA000006625_00605" }, { "type": "cds", "sequence": "contig_1", "start": 356922, "stop": 357800, "strand": "-", "frame": 2, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "EC:5.4.99.23", "KEGG:K06180", "SO:0001217", "UniParc:UPI000165F8BB", "UniRef:UniRef100_Q9PQH8", "UniRef:UniRef50_Q9PQH8", "UniRef:UniRef90_Q9PQH8" ], "nt": "ATGTCGTCTCAACGCATCGATAAATTTTTAGTAGAAAATACTAATTATTCACGAACATTAATTAAAAAAATGTTAGACCAAAACCTAATATTAGTTAATGACAATTTTATTAGTAAAGCAAGTTATTTATTAAAAAAGAATGATTATATTCAAATCAATAACGAACTACCTATAGTGCAAGAGCAAAAAAAATTGTTACCGTTTGTATATTCATTAGAAATTATCTTTGAAGATCAGGATCTATTGATCGTTAACAAACCTTCTGGTATGTTAGTTCATCCAACTAGTTTTCATGAGCAAAATACATTAGCAAACGCTTTAAAATATTATTTAAAAACTGATAATTTTTATATTACACATCGTTTGGATAAAAATACATCTGGATTAATTATAATTTGTAAAAATCAAATAGCGCTTAATTCTTTGCAACAACAATTTATGCAAAAAACCATAAGAAAAAGTTATTATGCTATTGTTCATAATCGTTTTGATATGCAGCATTTGCATTTTATGATCAATGAGCCTATTGGCCATAGTTATCAAGATAAATTAAGAATGAAAACAGGCAATTCTAAAAATACTAAAGAGGCTATCAGTATCGTTAAAGTACTTGAGCAATATAAAAATACCGCCCTTGTTGAAGTAAGTATTATTACTGGTAGAACTCATCAAATACGGGTTCATATGCGTTATATTAATCACCCTGTTTATAATGATCCTTTATATGGGATTAGTAAACATACCACAGAATACGAGCAATACTTGCATAGTTTTTATATTAGTTTTAATCATCCATCCACTAATAAAAAAGTAGAATTTAAAATCAATATGCCAAAAGAATTTATGGAATTAATTAGAGAGTTAAAAGCAAATGAATAG", "aa": "MSSQRIDKFLVENTNYSRTLIKKMLDQNLILVNDNFISKASYLLKKNDYIQINNELPIVQEQKKLLPFVYSLEIIFEDQDLLIVNKPSGMLVHPTSFHEQNTLANALKYYLKTDNFYITHRLDKNTSGLIIICKNQIALNSLQQQFMQKTIRKSYYAIVHNRFDMQHLHFMINEPIGHSYQDKLRMKTGNSKNTKEAISIVKVLEQYKNTALVEVSIITGRTHQIRVHMRYINHPVYNDPLYGISKHTTEYEQYLHSFYISFNHPSTNKKVEFKINMPKEFMELIRELKANE", "aa_hexdigest": "0b6d746320ae0bbfb5397df7a095b08b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8BB", "uniref100_id": "UniRef100_Q9PQH8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8BB", "UniRef:UniRef100_Q9PQH8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQH8", "uniref90_id": "UniRef90_Q9PQH8" }, "psc": { "uniref90_id": "UniRef90_Q9PQH8", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.23" ], "uniref50_id": "UniRef50_Q9PQH8", "kegg_orthology_id": "K06180" }, "pscc": { "uniref50_id": "UniRef50_Q9PQH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQH8" ], "product": "Pseudouridine synthase" }, "genes": [], "id": "GPKLONFDEH_629", "locus": "GCA000006625_00606" }, { "type": "cds", "sequence": "contig_1", "start": 358037, "stop": 358198, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTTTCTATTATTTGGCGGTTTAGGAAATATTATTGATCGCTCAATTCCAGAATTTGAATTATTCAATGCTTTACCAAATATATTTCACAATAGCCTTATAAATCCTAATAGTGTTGGTGGAGGCGATGATGTTATTAATGGTGTTGTTGATTATTGA", "aa": "MLFLLFGGLGNIIDRSIPEFELFNALPNIFHNSLINPNSVGGGDDVINGVVDY", "aa_hexdigest": "1bba94d8d9df5ebb72ab8efc60b7fd94", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5749.461600000002, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_630", "locus": "GCA000006625_00607" }, { "type": "cds", "sequence": "contig_1", "start": 358388, "stop": 358615, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCAAAAAATCAAAAATTCAAATTTTTATATAATAATTAAAAAATTTAATCAATCACTTAGAAAAATAAATTTTTTTGAGAAAATTATTTTAAAAATAAACAATAAAAGCATTCGTGATTACTTTTTATCAATAACATCATTAGATGTAATTTTTTATAAAATAATTAATTTTATTATTGTTGCTTTAATTGTGCTATTATCAGGTTTTTTGGTTCGTGAATGA", "aa": "MLQKIKNSNFYIIIKKFNQSLRKINFFEKIILKINNKSIRDYFLSITSLDVIFYKIINFIIVALIVLLSGFLVRE", "aa_hexdigest": "ae729c869640f11e0e2caabe2687866a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8958.769399999996, "isoelectric_point": 10.140183830261229 }, "id": "GPKLONFDEH_631", "locus": "GCA000006625_00608" }, { "type": "cds", "sequence": "contig_1", "start": 358816, "stop": 359160, "strand": "-", "frame": 1, "gene": null, "product": "inorganic diphosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B06C44" ], "nt": "ATGAAATTAAATGTAACTATTGAAATTCCAAAGAACTCAAATATTAAATATGAATATGATCGTGCAACTAAAGAAATCATGGTTGATCGTATCTTACACGGTTCAATGGTTTATCCTCATAATTATGGTTTTTTAAAAGAAGCATTAGATTATGATGGTGACGAATTGGATGTTTTGGTTTTTGCTGATCAAGCATTCCAACCAGGAATTAAAGTACCTGCACGTGTTTTGGGCGCCATGAAAATGATTGATGGAGGAGAAACAGATACTAAATTATTGGCAGTTATTGATGTTGATCCTCGTTATAAACACATTAATAATTTCAAAGATATTCCATTACATTGA", "aa": "MKLNVTIEIPKNSNIKYEYDRATKEIMVDRILHGSMVYPHNYGFLKEALDYDGDELDVLVFADQAFQPGIKVPARVLGAMKMIDGGETDTKLLAVIDVDPRYKHINNFKDIPLH", "aa_hexdigest": "c64f7c44daa3219150d1c25282f87cde", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021B06C44", "query_cov": 0.9824561403508771, "subject_cov": 0.8549618320610687, "identity": 0.509, "score": 103.0, "evalue": 2.05e-26, "valid": false, "product": "inorganic diphosphatase", "uniref50_id": "UniRef50_UPI0021B06C44" }, "pscc": { "uniref50_id": "UniRef50_UPI0021B06C44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B06C44" ], "product": "inorganic diphosphatase" }, "genes": [], "id": "GPKLONFDEH_632", "locus": "GCA000006625_00609" }, { "type": "cds", "sequence": "contig_1", "start": 359326, "stop": 359490, "strand": "+", "frame": 1, "gene": null, "product": "DUF3627 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B9", "UniRef:UniRef90_A0AAC9T0B9" ], "nt": "ATGAAAAATTTTTCAAAAAATAGAAATATTAATAATTTTAAAAACCCTTTAAGAGTTATTGAAGGAATTGTTCGTGAAATTAAAAATAATGAACTTCAAATTGATTTTGTTAATAGTCATAAAAAAGGAGTTTGTAAGTTTATTAATTTGACAGATTATCATTGA", "aa": "MKNFSKNRNINNFKNPLRVIEGIVREIKNNELQIDFVNSHKKGVCKFINLTDYH", "aa_hexdigest": "d36d07e1c8d0cba4f851955e7db45ee0", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 359326, "stop": 359706, "observations": { "mutation": [ 359488, 359572 ], "stop": [ 359488, 359572 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0B9", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 1.0, "score": 111.0, "evalue": 2.06e-30, "gene_start": 1, "gene_end": 54, "reference_start": 1, "reference_end": 54, "reference_sequence": "MKNFSKNRNINNFKNPLRVIEGIVREIKNNELQIDFVNSHKKGVCKFINLTDYHWNTISSRFLINSKHLFLVSKFDPIRHVYWLNYKIIHPIEIKNKRRSYPTLSHDRNLRIFLNNLLEKENELKN" }, "paralog": false, "identity": 0.9841269841269841, "score": 251.0, "evalue": 2.51e-88, "description": "Internal stop codon at 359488, 359572. Nonsense mutation around 359488, 359572.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0B9", "product": "DUF3627 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0B9", "UniRef:UniRef50_A0AAC9T0B9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B9" ], "product": "DUF3627 domain-containing protein" } }, "id": "GPKLONFDEH_633", "locus": "GCA000006625_00610" }, { "type": "cds", "sequence": "contig_1", "start": 359840, "stop": 360208, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGTATAATTTTAAACAATGAAAACAAAAAAAGAGAAGATAAAAACATTTTTTTGCTTAAAATTATTATTATGTTTAGAAATTTAATTAAGAATGTCTATCGTTATTTGCTAAAAAGCAAAGCCTCTTTTATAGGTCTTTGCCTTTTATTGTTTGTGTCAATCTTTAGTTTTAGTATGTTAACAAATCTTTCTAAAACATTAACAAGATCATATACAAACTTAGTCAATAGACAAAAATTACATGATATAACTGTTAATGAAAGTTATATTGAAGATCAAAAAGATATTAAACAAGCTCAATTCAATAAGCTTTTAGAAGAATTAAAAGTTTTTTATGCACAAAAAGGTTTGAATTTTGATTGA", "aa": "MKSIILNNENKKREDKNIFLLKIIIMFRNLIKNVYRYLLKSKASFIGLCLLLFVSIFSFSMLTNLSKTLTRSYTNLVNRQKLHDITVNESYIEDQKDIKQAQFNKLLEELKVFYAQKGLNFD", "aa_hexdigest": "14944b4233addfb9d0a4d1d88edbd772", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14475.918999999978, "isoelectric_point": 9.743251991271972 }, "id": "GPKLONFDEH_634", "locus": "GCA000006625_00611" }, { "type": "cds", "sequence": "contig_1", "start": 360224, "stop": 360565, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTTAATTCGACAAAAGATGATATTACTTATAAAATTGTTCAGTATTCAACAAGTAAAACGATTAATACATTCGATAAACAAACAAGTGAACGTTTGATTAAAAATAGTTTTTCTGATATTGATTTATATGGGATTTTAAATAATGCTAAAGTAGATGTTATTGATTTAATCAAAACTAAAACTTTAAATGCCAGTTTTGTCATTAATAATTTAAAAGAATTAGACAATAAACAAAAAAGTAATTTGGTAAAATTAGCTAAAGTTGATAATTACGATGTTAGTATTAGTCAACAAGCACGACTTTATTTATTAAAAAATATTGTTTATGGTGCATGA", "aa": "MIVNSTKDDITYKIVQYSTSKTINTFDKQTSERLIKNSFSDIDLYGILNNAKVDVIDLIKTKTLNASFVINNLKELDNKQKSNLVKLAKVDNYDVSISQQARLYLLKNIVYGA", "aa_hexdigest": "b80b313ba73e87508e3221032816c22f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12852.592399999983, "isoelectric_point": 9.247038841247559 }, "id": "GPKLONFDEH_635", "locus": "GCA000006625_00612" }, { "type": "cds", "sequence": "contig_1", "start": 360725, "stop": 360925, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATATCCATCAAAAAAAGGTATAAGTATAAATGGTAATGTTAATACTATTGTTAAAGGGCATCGAATAACTTTTAGTTTTGAACCCGCAGGGATTCCTATTCCAGTCTATTTTGATGTTAAATCTGCATACGCATTAGTTGGTTTAGGTGCTTTTTTGTCTAATCATAACAAACAAAAATACCCAATGTATTTATGA", "aa": "MLYPSKKGISINGNVNTIVKGHRITFSFEPAGIPIPVYFDVKSAYALVGLGAFLSNHNKQKYPMYL", "aa_hexdigest": "2211a27bf1f2a44160ced0e4be047f18", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7317.488500000002, "isoelectric_point": 9.756339073181149 }, "id": "GPKLONFDEH_636", "locus": "GCA000006625_00613" }, { "type": "cds", "sequence": "contig_1", "start": 360983, "stop": 361294, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGAAAAATATATTATTCATATTGATAACACACCTTATTTAATTTTAGATAGTGGTATTACTCCTGATTTTATGTATCCGATTATCTCATTCACTTCTGTTATTCCTAATCCAAAAAAAGAAGGACTAGTATATGTTAACTTAAATGGATATGCCCGTATTTACGATGCTTTTAGATCTAATCCAATTGAAAATAATTTACAAATTCGTATCACAAATCAAAAGCATCAATCCGTTGATATCAGTATTTTAAAAAAAATTGTTAAAAATTTAAATGATCAAATTAAAAAAGATGCTATCATGAACTGA", "aa": "MDEKYIIHIDNTPYLILDSGITPDFMYPIISFTSVIPNPKKEGLVYVNLNGYARIYDAFRSNPIENNLQIRITNQKHQSVDISILKKIVKNLNDQIKKDAIMN", "aa_hexdigest": "627b7564679e7924bdf52dd3ae63ebb3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11909.632499999987, "isoelectric_point": 7.934591484069824 }, "id": "GPKLONFDEH_637", "locus": "GCA000006625_00614" }, { "type": "cds", "sequence": "contig_1", "start": 361339, "stop": 361539, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTCAACGAGGAACACCATTAGCAATAGAAATACCAATATTAATTTTATTTTGTGCTAAAAAACGGTTGATGATGATAACAATTACAAAAATTGTTAGAATCAAAATAAATAAAGTTATTAAAACACTAAAAGTTTGTATTGTGTTCGTAATTTTGTTAATGAAAACTAATCTTAATGGGCTTGGTGTAATATGA", "aa": "MIFQRGTPLAIEIPILILFCAKKRLMMITITKIVRIKINKVIKTLKVCIVFVILLMKTNLNGLGVI", "aa_hexdigest": "98d4db916aef814cf9cb89c9195637a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7494.563399999998, "isoelectric_point": 10.78035488128662 }, "id": "GPKLONFDEH_638", "locus": "GCA000006625_00615" }, { "type": "cds", "sequence": "contig_1", "start": 361784, "stop": 362335, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGGAACTGAAACATTTAAAATTTCTAAAATTGTTTATGTAATTAAATTTCCATTTAAATATATTAGTGGTTTTAATAAGTATTTAATTACACTATCTTTTTCATCAATTACAAGAATTATTATTTTTTCAATTATGATTGCTTTATCATTTGGATCGATTATGTTTTTAACTGGAACAAGAGGTAAAATTAAACAAACTATAGATGCAACATTACAAACAAAAAAATATCGTTATGGAATTGATTTAATAACACCAACAGATCAAGGTGGTCAATATATTTCTTTAAGTACAAATCAAATTGGTTCTACATTAAATGATAATGATAATCATGAATTGACACAGAAATATGAAAGTAGTGATTATAAAGATTTTATTAATTCACCGTTATTTAAAAATTTACAGAATTTGAAGTTAATTGGTGCAAAAGATGACCAGCGTCGAATTTATGATTTAAACTATATTGCTAATGCCTTTCAAGTTAAGCAATTTTTAGATTTTAATTTTGGCATTGGTGATGTTAATAGTGGTAGTTCAAGTAATCCATGA", "aa": "MRGTETFKISKIVYVIKFPFKYISGFNKYLITLSFSSITRIIIFSIMIALSFGSIMFLTGTRGKIKQTIDATLQTKKYRYGIDLITPTDQGGQYISLSTNQIGSTLNDNDNHELTQKYESSDYKDFINSPLFKNLQNLKLIGAKDDQRRIYDLNYIANAFQVKQFLDFNFGIGDVNSGSSSNP", "aa_hexdigest": "f498af710d3052de500991a5294bee9e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20798.499099999986, "isoelectric_point": 9.396798896789551 }, "id": "GPKLONFDEH_639", "locus": "GCA000006625_00616" }, { "type": "cds", "sequence": "contig_1", "start": 362807, "stop": 363385, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTATGATGAAGTTTATTTTGAACGTAATAGCAAACAAAATTATCATAAACTAACTTATCAAACTTTTAGTTCTTATTTTAGAGCACGTTTTTTAAAATTTGATCCAGAAGGGGATTTTGATTATTATTTACCATTAAAATATTTTAATGCAACAATTGTTAGAAAAGAATTATTAGAGAATGCAATTAATAATGAAATTCTTTTTAAAAATGACGAAAATAAAATCATTAAAAAAATTAATTTTAAAAGTGATAATGATATAGTTGTTACTAAAATCAATGATTTAACTTATCAAGTTAAAAATATTATTAATCAACAGATTTATCTTTATTCTCCAAAACCCATTAAACGTTCTTATAGAATTGATCCAGCAAAAGCTACAAATGCTATTAAAACAGGATTATCTATTAAATTGGATAATGATTTTATTAATTTAGTATTATTAACTCATCCATCAATTAATCCATCGTATGCAGATGTTTGGTCAGGTATCACATTTAATATGGTAGGTTTTGATTATTTAACAAAAAATGATAAAACACAGTTAAACGAATTTGATGAACCTTATATTTGA", "aa": "MIYDEVYFERNSKQNYHKLTYQTFSSYFRARFLKFDPEGDFDYYLPLKYFNATIVRKELLENAINNEILFKNDENKIIKKINFKSDNDIVVTKINDLTYQVKNIINQQIYLYSPKPIKRSYRIDPAKATNAIKTGLSIKLDNDFINLVLLTHPSINPSYADVWSGITFNMVGFDYLTKNDKTQLNEFDEPYI", "aa_hexdigest": "292774c0d9e68b64d23f1d7caf7b57a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22765.614900000008, "isoelectric_point": 7.751781272888183 }, "id": "GPKLONFDEH_640", "locus": "GCA000006625_00617" }, { "type": "cds", "sequence": "contig_1", "start": 363476, "stop": 363895, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTTATTATCCAATAAAAAACAGATTAATAATTTATTATTTAATAAAAATGATGAGCAAGTAGCAATTATTAATCGAGTAGCTGCCAAATATTACAATTTAAAAATTGGTGATCATTTTAGTTTTGTTGCTAATAATCAAACAAATCGTTATACCAAAGATTACATAAAAAAACAAATCACTTTAAAAGTAGTTGGTATAATTGATACTTATCAGGGGATCGAAATTTATACAGGGATTAAAAATGCCGCTGAAATTTTAGGAATGCATAGTTCGCCATATGCTGATAAATCACTTTATGATGAATACGATGGTGCTTTCAATGGAATTTTTACAAATAAAAATAATCCAGCAATGTTAACACGTATTATTTCTATTTACTCACCTTCGGGACTATATCCTATTTCTGAAACATGA", "aa": "MHLLSNKKQINNLLFNKNDEQVAIINRVAAKYYNLKIGDHFSFVANNQTNRYTKDYIKKQITLKVVGIIDTYQGIEIYTGIKNAAEILGMHSSPYADKSLYDEYDGAFNGIFTNKNNPAMLTRIISIYSPSGLYPISET", "aa_hexdigest": "c7b6345620339e49f1316c49dd8f7e24", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15791.796299999989, "isoelectric_point": 8.981364631652834 }, "id": "GPKLONFDEH_641", "locus": "GCA000006625_00618" }, { "type": "cds", "sequence": "contig_1", "start": 364262, "stop": 364531, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTTTTAATATTATTTATTATTATATTAGTACTTGTTTTAATTTCTATTAATTCATTGTCTGATACATTAAAAGTCGCTAAATTACTAAAAAATATTGGTTATAGTGATTTAAAGAATGCTAAGTTATTTTTAATGGCTTTTTTACCATCATTAATAATTGGATCAATTATTTCAATTCCACTAGTTATTGTAGTAATGCAAGTATTTAGCGAAATAATATTTAATAGTTTAAATGTTTTATTAACAACTAGTTTTATTTGGTGA", "aa": "MIFLILFIIILVLVLISINSLSDTLKVAKLLKNIGYSDLKNAKLFLMAFLPSLIIGSIISIPLVIVVMQVFSEIIFNSLNVLLTTSFIW", "aa_hexdigest": "9d44ae97767b19be74f2c33f6f205ec0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9939.210899999993, "isoelectric_point": 9.303642082214353 }, "id": "GPKLONFDEH_642", "locus": "GCA000006625_00619" }, { "type": "cds", "sequence": "contig_1", "start": 364755, "stop": 365186, "strand": "+", "frame": 3, "gene": "nusA", "product": "NusA N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00291363D4", "UniRef:UniRef90_UPI00291363D4" ], "nt": "ATGTCTAACTCATTTAAATCAAAAGAATTTATTGAATACTTTAAAGATACAGCAAAACAAAATGAAATTGAATTTGAAGTTTTATCTTCAATTATTAAAGAAGCATTTGAAAAAGCTTATTTACGAACACATCCTGGTGAAAATTTTGAAACAAATATTGATCTTAAACAAGGAACAATTAATTGTTTTCGAAATTTAGTGGTCGTTGAGAATGAAAAAGTACATAATGAAGATTTAGAAACATGTTTGGATGATGCTGTTGAAATTTTATTAGATGATGCTCGTAAAATCAATGCAAATGCTCAAATTGGCGATCTTATTAAACAACATATTTCAATTGATAATTTTAAAAGTATTGAAGTAGGTCAAATTGGAAGTTTATTGCGTCAAAAAATTACTGAAATTCATAATAAAAGAGTAGCTGATTTTTGA", "aa": "MSNSFKSKEFIEYFKDTAKQNEIEFEVLSSIIKEAFEKAYLRTHPGENFETNIDLKQGTINCFRNLVVVENEKVHNEDLETCLDDAVEILLDDARKINANAQIGDLIKQHISIDNFKSIEVGQIGSLLRQKITEIHNKRVADF", "aa_hexdigest": "90b96ca1f03a05233b89943623c1a2be", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00291363D4", "query_cov": 0.8531468531468531, "subject_cov": 0.8356164383561644, "identity": 0.9840000000000001, "score": 232.0, "evalue": 1.58e-76, "valid": true, "product": "NusA N-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI00291363D4" }, "pscc": { "uniref50_id": "UniRef50_UPI00291363D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00291363D4" ], "product": "NusA N-terminal domain-containing protein" }, "genes": [ "nusA" ], "id": "GPKLONFDEH_643", "locus": "GCA000006625_00620" }, { "type": "cds", "sequence": "contig_1", "start": 365199, "stop": 365291, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGATTCGTGCAAAAGTTGCAGAAGTTAATTATAACAAACAACGTAATGAAATTACTGGAGTAAAAGTTGAACTAGATGACCAATGA", "aa": "MKMIRAKVAEVNYNKQRNEITGVKVELDDQ", "aa_hexdigest": "4c024f2fd3a20a93f8704a1cae5ebe31", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3521.0319, "isoelectric_point": 8.139987754821778 }, "id": "GPKLONFDEH_644", "locus": "GCA000006625_00621" }, { "type": "cds", "sequence": "contig_1", "start": 365961, "stop": 366131, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTTAATTGATAGTATAGAAGATGATAAAATTGAACAAGATGTCCAATTAAATCACCCATTAAAACAACAAGAAAAAAAAGTTGTTTCTTCAAATGATGAAGATGGTGAAAATGATGAAACACAATTAGAATATCTTGAAGGATTCGAAGATTTTAAATTCTAA", "aa": "MLALIDSIEDDKIEQDVQLNHPLKQQEKKVVSSNDEDGENDETQLEYLEGFEDFKF", "aa_hexdigest": "7b912fa161d2d0e720f1de2e653d9474", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6558.0286, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_645", "locus": "GCA000006625_00622" }, { "type": "cds", "sequence": "contig_1", "start": 366142, "stop": 366393, "strand": "+", "frame": 1, "gene": "ylxR", "product": "YlxR domain-containing protein", "db_xrefs": [ "RefSeq:WP_006688432.1", "SO:0001217", "UniParc:UPI00000C1C18", "UniRef:UniRef100_A0AAC9X6P2", "UniRef:UniRef50_A0AAC9X6P2", "UniRef:UniRef90_A0AAC9X6P2" ], "nt": "ATGGCAATGAAAGTTAATGAAAGAACTTGTGTTTTTAGTAAAGAAAAATATCCTAAAGATCATTTAGTTCGCTTTGTTATTATTAACGGTGAGTTAATTTTTAGTTTGAATTATCATCGTGGGTATTATTTAATGATTCAAAAGAATACAAACATTCAAAAGGTGTGTCAATTTCTAAAAAAACGTTTTATGATTAAAAACGAAGAACAAGTTTATCGGATTTTAGAGCAATTAATTCAATCATTAATTTAA", "aa": "MAMKVNERTCVFSKEKYPKDHLVRFVIINGELIFSLNYHRGYYLMIQKNTNIQKVCQFLKKRFMIKNEEQVYRILEQLIQSLI", "aa_hexdigest": "05789dfa2d41348fecc04d807132c00a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C18", "ncbi_nrp_id": "WP_006688432.1", "uniref100_id": "UniRef100_A0AAC9X6P2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C18", "RefSeq:WP_006688432.1", "UniRef:UniRef100_A0AAC9X6P2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6P2", "uniref90_id": "UniRef90_A0AAC9X6P2" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X6P2", "product": "YlxR domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6P2" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6P2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6P2" ], "product": "YlxR domain-containing protein" }, "genes": [ "ylxR" ], "id": "GPKLONFDEH_646", "locus": "GCA000006625_00623" }, { "type": "cds", "sequence": "contig_1", "start": 366428, "stop": 367939, "strand": "+", "frame": 2, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413", "KEGG:K02519", "SO:0001217", "UniRef:UniRef50_Q9PQH1", "UniRef:UniRef90_Q9PQH1" ], "nt": "ATGGCGAAAAAAAATATCAAACAAAAGAAAGATAATCGGATAGCAATCGATGTAAAAAAACACATTAAAAAAGTTGATGTTGGGGTTTTTGATGGTACATTTGTTTTCACATCACCTCTTAGTATTTCAGAATTAGCACCAAAACTAAATAAAAGTCCAAACGAAATTATTATGCGTTATTTCAAAAAAGGTGTTGTTTATAACTTAAACACTATTTTAGATGAAGAACAAATTGGTGAGTTATGTTTAGAATATGATTTAGATTTTAAAATTGAAAAGAATGTTAACACTGAAAATCTTTTAGAAAATATTTATTTTGATGATCTAGAAATTGATTTGGTTGCTCGTGCTCCAATTGTAACAATTATGGGTCATGTTGATCATGGTAAAACAACACTTTTAGATACTATTCGTAAATCATCAATAACAGCATCAGAAGCTGGAGGAATTACTCAACATATTGGAGCTTATCAAATTATAAAAGACAATAGAGCAATTACTTTTATTGATACACCCGGACATGAAGCCTTTACTGAAATGCGTGCTCGCGGAGCAAATTTAACAGATATTGTGATTTTAGTTGTTGCTGCTGATGATGGTATTAAAATGCAAACTGAAGAAGCAATTGATCATGCTAAAGCTGCTAATGTTCCTATTATTGTTTTTGTTAATAAAATGGATAAATACGAAGCAAATCCTGAAAAAGTTTTAAATCAATTAAGTGCTAAAGAAATTGTTGCTGAAGAGCTTGGTGGTGATGTTGTTTTTGTTAAAGGCTCAGCATTGAAAAATGAAGGAATTTCTGAATTATTAGATTCAATTTTATTGATTGCTGAATTAAATAATTACAAAGCTAATCCTAATCGTTTAGCTTATGGAACTACAATTGAAGCAAATTTGGATAAAGGTCATGGTCCTCTTGCTACATTGTTAGTTCAAAATGGCACATTACGAAAAGGTGATTATTTAGTTGTTGGTTCAACATATGGAAAAATTCGTAATATGTTTGATGAATATGACAACGAAATTGAAATAGCATTACCTTCAAAACCTGTTAAAGTTTCTGGATTTGAAGAAGTACCAACAGCCGGTGATAAATTTTTAGCTTTAGCTGATGAAAAACAAGCACGTGCAATAGCAAATGATGTTAAACAAAAGAAAATGCGTTTAGAACGTGCAATGTTACAAAGTTCGGATATTCGAACAAAAATTGCTAATGGTGAATTAAAAAATATTAATTTAATTATTAAAGCTGATGTTCAAGGTTCACTTGAAGCTTTAAAAGGAATTTTTAGTAGCATTAACATTGAAGGTGTGACTACGACACTAATACGGAGTGCAATTGGAACAATTAGTGAAAGTGATGTGCGTTTAGCACAGACTTCTGATGCAATAATTATTGGTTTTAATGTCCGTGCTAGTCGAATTATTAAAGATTTAGCTGATTCAGTCGGTGTACAAATTATGAATTATGACATTATTTATAAATTTAAAGAAGATTTAGAATTATGA", "aa": "MAKKNIKQKKDNRIAIDVKKHIKKVDVGVFDGTFVFTSPLSISELAPKLNKSPNEIIMRYFKKGVVYNLNTILDEEQIGELCLEYDLDFKIEKNVNTENLLENIYFDDLEIDLVARAPIVTIMGHVDHGKTTLLDTIRKSSITASEAGGITQHIGAYQIIKDNRAITFIDTPGHEAFTEMRARGANLTDIVILVVAADDGIKMQTEEAIDHAKAANVPIIVFVNKMDKYEANPEKVLNQLSAKEIVAEELGGDVVFVKGSALKNEGISELLDSILLIAELNNYKANPNRLAYGTTIEANLDKGHGPLATLLVQNGTLRKGDYLVVGSTYGKIRNMFDEYDNEIEIALPSKPVKVSGFEEVPTAGDKFLALADEKQARAIANDVKQKKMRLERAMLQSSDIRTKIANGELKNINLIIKADVQGSLEALKGIFSSINIEGVTTTLIRSAIGTISESDVRLAQTSDAIIIGFNVRASRIIKDLADSVGVQIMNYDIIYKFKEDLEL", "aa_hexdigest": "c05a49accf48ea7381c1682643a79e02", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q9PQH1", "query_cov": 1.0, "subject_cov": 0.8192182410423453, "identity": 1.0, "score": 954.0, "evalue": 0.0, "valid": true, "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_Q9PQH1", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQH1" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "GPKLONFDEH_647", "locus": "GCA000006625_00624" }, { "type": "cds", "sequence": "contig_1", "start": 367940, "stop": 368272, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03144.31" ], "nt": "ATGAAAGGTACATTAGACCCAATTATTATTGAAGAAGTGATTGGCGAAGCCAAAGTTCTTAAATTATTTAAACACAGTCAAGTTGGAACAATTTGTGGTTGTCGAGTAATTAATGGTAAAATTAAAAGAAATGCTTTGGTTCGTGTTTTACGTGATGGTATTGTTATATATAATTCAAAAATTGCGACATTACAACACAATAAAGATAGTGTTAATGAAGTTATTGCTGATAAAGAATGCGGATTAACAATTGCAAACTTTAATGATATTAAAGAGAACGATATTATTGAAGTTTATATTAAAGTTGAAAAGAAACATGATGAGGTTAAATAA", "aa": "MKGTLDPIIIEEVIGEAKVLKLFKHSQVGTICGCRVINGKIKRNALVRVLRDGIVIYNSKIATLQHNKDSVNEVIADKECGLTIANFNDIKENDIIEVYIKVEKKHDEVK", "aa_hexdigest": "c35aae2f9064afb53beb8df58b8a6420", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03144.31", "name": "GTP_EFTU_D2", "length": 66, "aa_cov": 0.5545454545454546, "hmm_cov": 0.9848484848484849, "evalue": 1.7046357963831097e-09, "score": 33.62324523925781, "start": 29, "stop": 89 } ], "seq_stats": { "molecular_weight": 12356.331999999988, "isoelectric_point": 8.42590503692627 }, "id": "GPKLONFDEH_648", "locus": "GCA000006625_00625" }, { "type": "cds", "sequence": "contig_1", "start": 368280, "stop": 368525, "strand": "+", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024", "KEGG:K02834", "SO:0001217", "UniRef:UniRef50_Q9PQH0", "UniRef:UniRef90_Q9PQH0" ], "nt": "ATGGCAAACGAAGTTCGAGTTGCAAGGTTAGAATCTTTAATTAAAGATGTTATTAATAATGCGTTAGCGAATGAAATTAATGATAAAATTGCAAAATTAGCACGTGTAACTGCAGTGCGTTTAAGTAATGATTTATCAGTAGCAAAGATTTTTTTAGACGCTCATAAACGCGAAAGTATGCTTAAAGTTTTAGAAAATGTTAATAAAGTTAGTGGCTTATTGCGTTCAAAATTAGCTGCGGAATGA", "aa": "MANEVRVARLESLIKDVINNALANEINDKIAKLARVTAVRLSNDLSVAKIFLDAHKRESMLKVLENVNKVSGLLRSKLAAE", "aa_hexdigest": "283c59ce929c0fa36c07a2d3f257d6e9", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [ "rbfA" ], "truncated": "3-prime", "pseudogene": { "start": 368280, "stop": 368630, "observations": { "mutation": [ 368523 ], "stop": [ 368523 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQH0", "query_cov": 1.0, "subject_cov": 0.6982758620689655, "identity": 1.0, "score": 148.0, "evalue": 8.03e-45, "gene_start": 1, "gene_end": 81, "reference_start": 1, "reference_end": 81, "reference_sequence": "MANEVRVARLESLIKDVINNALANEINDKIAKLARVTAVRLSNDLSVAKIFLDAHKRESMLKVLENVNKVSGLLRSKLAAEWTSYKVPELRFVIDETIDYANHIDELFKKIKQQEN" }, "paralog": false, "identity": 0.9913793103448276, "score": 216.0, "evalue": 3.09e-74, "description": "Internal stop codon at 368523. Nonsense mutation around 368523.", "psc": { "uniref90_id": "UniRef90_Q9PQH0", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_Q9PQH0", "cog_id": "COG0858", "cog_category": "J", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQH0", "UniRef:UniRef50_Q9PQH0", "COG:COG0858", "COG:J", "KEGG:K02834", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQH0" ], "product": "Ribosome-binding factor A" } }, "id": "GPKLONFDEH_649", "locus": "GCA000006625_00626" }, { "type": "cds", "sequence": "contig_1", "start": 369575, "stop": 369823, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGTTTTGTTAAAGAATTAAATTTTGAAATTAATCAAAAAAAATATTTTATTAAAATTTATTTACAACCAAGAATTAAATATATTAAAATCAAAATCATTAATCATCAAATTGTTTGTTTGATCAATCACTTAAAATTAATTAATATTGCTGAAGAATTTATTAAAAATAATAAGTTAAAAATTTTAAAAATATATGAAAATGACTTAAAGAAAATTAAGTATGATGAACAATCTTTAGATTGA", "aa": "MLSFVKELNFEINQKKYFIKIYLQPRIKYIKIKIINHQIVCLINHLKLINIAEEFIKNNKLKILKIYENDLKKIKYDEQSLD", "aa_hexdigest": "0915a95fad009a602945605414581ed1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10037.95529999999, "isoelectric_point": 9.54804172515869 }, "id": "GPKLONFDEH_650", "locus": "GCA000006625_00627" }, { "type": "cds", "sequence": "contig_1", "start": 369836, "stop": 371635, "strand": "-", "frame": 3, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "COG:COG0481", "COG:J", "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "RefSeq:WP_006688644.1", "SO:0001217", "UniParc:UPI000012E493", "UniRef:UniRef100_Q9PQG7", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_Q9PQG7" ], "nt": "ATGGATAAAAAATTCATCCGTAATTTTAGTATTATTGCCCATATTGATCATGGGAAGTCAACATTATCTGATCGAATTATTGAATTTACAAATACTTTGAGTAAACGTGAAATGACAAACCAAATTTTAGATTCTATGGATATAGAACGTGAACGTGGAATTACTATTAAATTAAATGCTGTACAAATTAAATATCATGCTAAAGATAAACATGAATATTTAATTCACTTAATTGACACTCCCGGTCACGTCGATTTTACTTACGAAGTTTCACGAAGTTTAGCAGCATGTGAGGGAGCAATTCTAGTTGTTGATGCTGCACAAGGAATTGAAGCCCAAACTTTATCTAATGTTTACTTAGCATTAGAAAACAATTTAGAGATTGTTCCAACAATTAATAAAATTGATTTACCATCAGCAGACCCTAATCGTGTTAAAAAAGAAATCGAAGACGTTATTGGTTTAGATACAGCGGATGTGCCTTTAATTTCAGCAAAAACGGGGTTAAACATTCAAGATGTATTAGAAGCAATAATTAAACACGTTCCACCTCCATTAGATGCTAAAGATGATGCTAAATTACAAGCCTTAATATTTGATTCTTTTTATGATTCTTATAAAGGTGTTGTTTGTTTAGTACGTGTAAAACAAGGAACAATTAAAGTTGGAGATAAAATTCGTATGATGGCTAACAATAAAGATTATATTGTTAGTGAATTAGGAATTAGAACACCAAAAATTGTTAATAAAACAGAATTGGTAGCCGGAGAAGTTGGTTGGGTAGCTGCAGCTATCAAAACAGTCAAAGATATTAATGTGGGTGATACTATTACTCATACCAATAATCCTGCTGATAAACCACTACCAGGTTATAAAAAAATTTTACCAATGGTTTATTGTGGCTTATACCCAATTGATACTAGTCAATATGATGATTTAAAAGAAGCTATGGCTAAAATCTCCTTATCTGATGCTGCATTAACTTATGAATATGAAACATCGCAAGCACTAGGATTTGGAATTCGTTGTGGATTTTTAGGTTTATTACATATGGATGTTATTCGTGAACGAATTGCTCGTGAATTTAATATTGAATTAATTTTAACTGCCCCTTCAGTAATATATAAAATTGAATTAACAAATAATCAAGAAATTAGTATTGATAGTCCTGCTAAAATGCCTGAACCAACTAGTATTAAACTTATTAAAGAACCATTTGTTAAATTAGCAATCATTACACCAGATAATTATGTTGGTGCAATTATGGAATTATGTCAGTCCCGAAGAGGAATATATGATGATCTAGAAGTTATTGACGGTACAAGACGAAAATTAATTTATAAAATGCCATTAGCTGAAATTATGTACAGTTTTTTTGATTCACTAAAATCAATTACTAAAGGATATGCAACAATGGACTATGAACTAATTGGTTACCAAGCTGAAAAATTAGTCAAAATCGATATTATGTTAAATGGTAATAAAGTTGATGCTTTAAGTATTATTGCTCATCGTGATTTTGCTTATGGGAAGTCAAAAATTATTTGTGAACGTCTAAAAGAGGTTATTCCAAAACATCAATTTGAAATTCCTATTCAAGCTTCAATTGGTTCAAAAATTATTGCTCGTGAAACTATTAAAGCAGTACGCAAGGATGTGATTGCTAAATGCTATGGTGGAGATGTTTCAAGAAAGAAAAAACTTCTAGAACAACAAAAAGAAGGTAAAAAACGTCTAAAAGCGATTGGTAATGTTGATGTTCCTCAAGATGCTTTTGTAAAAGTTTTAAGTGAAAACTAA", "aa": "MDKKFIRNFSIIAHIDHGKSTLSDRIIEFTNTLSKREMTNQILDSMDIERERGITIKLNAVQIKYHAKDKHEYLIHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLSNVYLALENNLEIVPTINKIDLPSADPNRVKKEIEDVIGLDTADVPLISAKTGLNIQDVLEAIIKHVPPPLDAKDDAKLQALIFDSFYDSYKGVVCLVRVKQGTIKVGDKIRMMANNKDYIVSELGIRTPKIVNKTELVAGEVGWVAAAIKTVKDINVGDTITHTNNPADKPLPGYKKILPMVYCGLYPIDTSQYDDLKEAMAKISLSDAALTYEYETSQALGFGIRCGFLGLLHMDVIRERIAREFNIELILTAPSVIYKIELTNNQEISIDSPAKMPEPTSIKLIKEPFVKLAIITPDNYVGAIMELCQSRRGIYDDLEVIDGTRRKLIYKMPLAEIMYSFFDSLKSITKGYATMDYELIGYQAEKLVKIDIMLNGNKVDALSIIAHRDFAYGKSKIICERLKEVIPKHQFEIPIQASIGSKIIARETIKAVRKDVIAKCYGGDVSRKKKLLEQQKEGKKRLKAIGNVDVPQDAFVKVLSEN", "aa_hexdigest": "efba3de470c87a405a530d1dbd673c59", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000012E493", "ncbi_nrp_id": "WP_006688644.1", "uniref100_id": "UniRef100_Q9PQG7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E493", "RefSeq:WP_006688644.1", "UniRef:UniRef100_Q9PQG7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQG7", "uniref90_id": "UniRef90_Q9PQG7" }, "psc": { "uniref90_id": "UniRef90_Q9PQG7", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "cog_id": "COG0481", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" }, "genes": [ "lepA" ], "id": "GPKLONFDEH_651", "locus": "GCA000006625_00628" }, { "type": "cds", "sequence": "contig_1", "start": 371753, "stop": 371875, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTCAATTTATGACTGTTATCGTTGCTGAAAAATTAAAACTTGAAACTTTCCCAATTTATGCTGCAAACAAACAAGAGCGTAAAGATGTAGATATGAAATCACTGTCAGATTTTTAA", "aa": "MIFQFMTVIVAEKLKLETFPIYAANKQERKDVDMKSLSDF", "aa_hexdigest": "aff05fe5fd67c25591fded06cdc847e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4726.5340000000015, "isoelectric_point": 6.060707664489747 }, "id": "GPKLONFDEH_652", "locus": "GCA000006625_00629" }, { "type": "cds", "sequence": "contig_1", "start": 371882, "stop": 372148, "strand": "-", "frame": 3, "gene": "lemA", "product": "LemA family protein", "db_xrefs": [ "COG:COG1704", "COG:M", "KEGG:K03744", "SO:0001217", "UniRef:UniRef50_A0A084F1M3", "UniRef:UniRef90_A0AAC9T3D1" ], "nt": "ATGATTGAACAAACTAAATCATATTACAAATATGAAGAAAAAGTATTAGATGAAATAACTAAAAATCGAAGTATTCAAATGTCAAATGATATTAACAAAAATGAAGAAGTATTAAATAAACTTCAAAATTTAGTTAATGTTCAATTCGAACGTTATCCAGATTTGAAATCATCCAATATTTTAATGGAATTGATGAGTACATCAAGTTATTTAGAAAATGAAATTGCTTCATCACGACGTGCTTATAACTCACGTGCAACTGATTGA", "aa": "MIEQTKSYYKYEEKVLDEITKNRSIQMSNDINKNEEVLNKLQNLVNVQFERYPDLKSSNILMELMSTSSYLENEIASSRRAYNSRATD", "aa_hexdigest": "2bd33dda5aad92faee38fb26030790fb", "start_type": "ATG", "rbs_motif": null, "genes": [ "lemA" ], "truncated": "both", "pseudogene": { "start": 371753, "stop": 372412, "observations": { "mutation": [ 371884, 372253, 372256, 372274, 372403 ], "stop": [ 371884, 372253, 372256, 372274, 372403 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3D1", "query_cov": 1.0, "subject_cov": 0.4018264840182648, "identity": 1.0, "score": 159.0, "evalue": 9.57e-48, "gene_start": 1, "gene_end": 88, "reference_start": 89, "reference_end": 176, "reference_sequence": "MDLWKEQDENGISPNVSNERKVVVASNGEKTLYIFIIVISIISIIGWIFLLVWWFNTKNHLIQTKNSVNEASSSIQVAQTKRFDLLNKMIEQTKSYYKYEEKVLDEITKNRSIQMSNDINKNEEVLNKLQNLVNVQFERYPDLKSSNILMELMSTSSYLENEIASSRRAYNSRATDWNIMIFQFMTVIVAEKLKLETFPIYAANKQERKDVDMKSLSDF" }, "paralog": false, "identity": 0.9771689497716894, "score": 402.0, "evalue": 1.02e-145, "description": "Internal stop codon at 371884, 372253, 372256, 372274, 372403. Nonsense mutation around 371884, 372253, 372256, 372274, 372403.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3D1", "gene": "LemA", "product": "LemA family protein", "uniref50_id": "UniRef50_A0A084F1M3", "cog_id": "COG1704", "cog_category": "M", "kegg_orthology_id": "K03744", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3D1", "UniRef:UniRef50_A0A084F1M3", "COG:COG1704", "COG:M", "KEGG:K03744" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1M3" ], "product": "LemA family protein" } }, "id": "GPKLONFDEH_653", "locus": "GCA000006625_00630" }, { "type": "cds", "sequence": "contig_1", "start": 372558, "stop": 372917, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688471.1", "SO:0001217", "UniParc:UPI00000C1C1C", "UniRef:UniRef100_A0AAC9T0Y0", "UniRef:UniRef50_B5ZBD6", "UniRef:UniRef90_A0AAC9T0Y0" ], "nt": "ATGGAAAAGACAATAATTCAATTGGAATTTAATGAAGAAGAATTAGCATCAATAAAAAAAATTAAAGATAAAATGGATCCTAATAATAATTTAGAATTAAATGTATTTTTAAAAGATTTAATTAAGGATTTAGCGCGTGATTATTTAAATTTTTCAAACCAATCATTTGAAAGTATTGCAAAGCAAATGAATGATTTAAAAGATCTAATAGGAAACGTAGCGAACGATCCATCTAGTTTCGATTTTAATTCAGTAATGAATGAATTCCAAAAGTTTAGTAAATCACAAAAGGATGAAGAAAAAGAAATAAAAAACGATACTGAAATAAAAACTGCGACTAAACCTACTAAAAAATCTTAA", "aa": "MEKTIIQLEFNEEELASIKKIKDKMDPNNNLELNVFLKDLIKDLARDYLNFSNQSFESIAKQMNDLKDLIGNVANDPSSFDFNSVMNEFQKFSKSQKDEEKEIKNDTEIKTATKPTKKS", "aa_hexdigest": "a322ace84484d5ffeda17d8bbe431290", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1C1C", "ncbi_nrp_id": "WP_006688471.1", "uniref100_id": "UniRef100_A0AAC9T0Y0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C1C", "RefSeq:WP_006688471.1", "UniRef:UniRef100_A0AAC9T0Y0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0Y0", "uniref90_id": "UniRef90_A0AAC9T0Y0" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0Y0", "uniref50_id": "UniRef50_B5ZBD6" }, "pscc": { "uniref50_id": "UniRef50_B5ZBD6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBD6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13813.42549999998, "isoelectric_point": 4.968830680847168 }, "id": "GPKLONFDEH_654", "locus": "GCA000006625_00631" }, { "type": "cds", "sequence": "contig_1", "start": 372921, "stop": 373694, "strand": "+", "frame": 3, "gene": "spoU", "product": "rRNA methylase", "db_xrefs": [ "COG:COG0566", "COG:J", "RefSeq:WP_006688821.1", "SO:0001217", "UniParc:UPI00000C1C1D", "UniRef:UniRef100_Q9PQG4", "UniRef:UniRef50_A0AAP9AA20", "UniRef:UniRef90_Q9PQG4" ], "nt": "ATGTTAAAAATTATTACATCTAATAAAAATGAAGAAATTATTCATTTTTATCGTTTAATTCATGATAAAAAATATCGCGAAGCTAATAAATTATTCATAATTGAGGGTTTTAAATTATTAGATGAAGCTTTGCAAAATCAACAAACAATAGTTTCAATTATTATTAATGAACATGTTTATAATAAAGAATTTGAAACGATTTTTAAATATACATTATATGACAATATTCAAATTTATAAAGTTAGTGGAAATATTATTAAAAAACTATCAGTTAATATCGAACCAAGTCCAATTTTAGCAGTTGTTAAAATAAAACAAGAAATGTGTGATATTAACGATAATATTTTATTGTTAGATAATATTCAAGATCCAGGTAATTTAGGAACCATTTTAAGAACCTGCTTTGCTTTCAATATTAAACAAGTTATTTTTAATAATTGTGTTGATTTATATAATCCTAAAACAATTAAAGCAAGTATGGGGGCCATTTTTAAAATTAATTTTTTACGTTTTAATGATTCTAAACTAGCATTAAATTTTTTATTAAAAAATAAATTCTATTTAATTGCTACTATTTTAGATAAAAACGCAATTTCTTTAAACGAAATAACGATTAAACAAAAATATTGTTTAATGGTAGGTAATGAAGGTCATGGTTTGGTACAAACTTTTATTGATCATGCTCACATTAAAACAATGATTAAAATGAATCAAACAGAATCATTAAATGTTGCAGTAGCAGCAGCAATTTTTTTATATGAACTTAATAAATAA", "aa": "MLKIITSNKNEEIIHFYRLIHDKKYREANKLFIIEGFKLLDEALQNQQTIVSIIINEHVYNKEFETIFKYTLYDNIQIYKVSGNIIKKLSVNIEPSPILAVVKIKQEMCDINDNILLLDNIQDPGNLGTILRTCFAFNIKQVIFNNCVDLYNPKTIKASMGAIFKINFLRFNDSKLALNFLLKNKFYLIATILDKNAISLNEITIKQKYCLMVGNEGHGLVQTFIDHAHIKTMIKMNQTESLNVAVAAAIFLYELNK", "aa_hexdigest": "dcb907d59f6fae2badf92eb3ec8ab0f5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C1D", "ncbi_nrp_id": "WP_006688821.1", "uniref100_id": "UniRef100_Q9PQG4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C1D", "RefSeq:WP_006688821.1", "UniRef:UniRef100_Q9PQG4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQG4", "uniref90_id": "UniRef90_Q9PQG4" }, "psc": { "uniref90_id": "UniRef90_Q9PQG4", "gene": "SpoU", "product": "rRNA methylase", "uniref50_id": "UniRef50_A0AAP9AA20", "cog_id": "COG0566", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AA20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AA20" ], "product": "RNA methyltransferase" }, "genes": [ "spoU" ], "id": "GPKLONFDEH_655", "locus": "GCA000006625_00632" }, { "type": "cds", "sequence": "contig_1", "start": 373678, "stop": 373884, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTAATAAATAAAGAACTTTTTTTAATTACTGGTGACAATCGTTTAAAAATGGATCAAAAAATTAATGAAATTGCGACCTCTTTTGATGAATTAGTTAAAATTAATGATCAAGAAATTTCATTAATTTCTTTTAAAAATCTTATTGAACAAGATGATTTATTCAATAGTAATAAGATCTACTTATTTAAAAACGTTAACTGA", "aa": "MNLINKELFLITGDNRLKMDQKINEIATSFDELVKINDQEISLISFKNLIEQDDLFNSNKIYLFKNVN", "aa_hexdigest": "17ffd9429bf363ad368d08cccb94572b", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8008.094299999996, "isoelectric_point": 4.691456413269043 }, "id": "GPKLONFDEH_656", "locus": "GCA000006625_00633" }, { "type": "cds", "sequence": "contig_1", "start": 374041, "stop": 374607, "strand": "+", "frame": 1, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "SO:0001217", "UniRef:UniRef50_Q9PQG3", "UniRef:UniRef90_Q9PQG3" ], "nt": "ATGACATATACAAATGAAAATGCTATGGCTTTCTTAAAAGAAGAGTTAAATCAGCGTAATTTATCATTAAATAAATCTATAATGCAAACGATTATTCAAAAAACAAATTTTAATATTAATTTTCTTAATAATGAATTAGATAAAATAGAATTAATTAATGATTTTTTAAAAAATCATGGTATGTTTGATATTAATAATTTTATATGTGATTATGGTGAATATCAAATTTTTAGTTTATTAAATCTTTTGTATCAACATAAAATAAATGAATCAATAAATTTAATTAATAAAATGCTCATTGACAAAATTGATGAACTCACAATAATTAATATGTTAGCGACAATAATGAGTACACATTACTTAATTAAATTATTACATGAAAAAAAATATAACAAAAACGTTATATCTAGCTCTTTAAATCAAAAACCTTATATTATTGATTTAAATTTAAAATTAATTAGAAATTATTCAGCTAAATTTTTACTTAAAAAAATGTATGATTTATTACAATTAGAAATTAAAGTCAAGGAAAATAAAATTGATAAATATTTTGGATTAATTAATTGA", "aa": "MTYTNENAMAFLKEELNQRNLSLNKSIMQTIIQKTNFNINFLNNELDKIELINDFLKNHGMFDINNFICDYGEYQIFSLLNLLYQHKINESINLINKMLIDKIDELTIINMLATIMSTHYLIKLLHEKKYNKNVISSSLNQKPYIIDLNLKLIRNYSAKFLLKKMYDLLQLEIKVKENKIDKYFGLIN", "aa_hexdigest": "47fd851bec0485ec8b2d5cc6505c21ea", "start_type": "ATG", "rbs_motif": null, "genes": [ "holA" ], "truncated": "both", "pseudogene": { "start": 373741, "stop": 374622, "observations": { "mutation": [ 373882, 374605 ], "stop": [ 373882, 374605 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQG3", "query_cov": 1.0, "subject_cov": 0.5987261146496815, "identity": 1.0, "score": 357.0, "evalue": 9.72e-123, "gene_start": 1, "gene_end": 188, "reference_start": 122, "reference_end": 309, "reference_sequence": "MNLINKELFLITGDNRLKMDQKINEIATSFDELVKINDQEISLISFKNLIEQDDLFNSNKIYLFKNVNWFENLEHLKNVSDLIDYFFNNHVAIIITIESTKISTAKKIQETIAKFNHQITFMTYTNENAMAFLKEELNQRNLSLNKSIMQTIIQKTNFNINFLNNELDKIELINDFLKNHGMFDINNFICDYGEYQIFSLLNLLYQHKINESINLINKMLIDKIDELTIINMLATIMSTHYLIKLLHEKKYNKNVISSSLNQKPYIIDLNLKLIRNYSAKFLLKKMYDLLQLEIKVKENKIDKYFGLINWVLNF" }, "paralog": false, "identity": 0.9931740614334471, "score": 568.0, "evalue": 2.62e-208, "description": "Internal stop codon at 373882, 374605. Nonsense mutation around 373882, 374605.", "psc": { "uniref90_id": "UniRef90_Q9PQG3", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q9PQG3", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQG3", "EC:2.7.7.7", "UniRef:UniRef50_Q9PQG3", "COG:COG1466", "COG:L", "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQG3" ], "product": "DNA-directed DNA polymerase" } }, "id": "GPKLONFDEH_657", "locus": "GCA000006625_00634" }, { "type": "cds", "sequence": "contig_1", "start": 374779, "stop": 374985, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATGCAGAATTGTCAAGTGCACAAAAAGATTTATATAAATCAATTAAATTATATTTAAACACAATTTTTTTGAATCAAAAACTAAATTATGCTATTAAAAATGATAATGATTATCGAAATCAATTAAATAAAGTAATGAATATTTTTGAATATTATGAAGATAATTATGAAATAGCAAATAAAAATTTTCATAGTTATTGA", "aa": "MLNAELSSAQKDLYKSIKLYLNTIFLNQKLNYAIKNDNDYRNQLNKVMNIFEYYEDNYEIANKNFHSY", "aa_hexdigest": "49e2e79e23d6a63b11a4a31a89db7f21", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8252.174299999999, "isoelectric_point": 6.539405632019044 }, "id": "GPKLONFDEH_658", "locus": "GCA000006625_00635" }, { "type": "cds", "sequence": "contig_1", "start": 375172, "stop": 375654, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTTTATACTCCAAGAAATCAAGAAAATAATACTAGTACAGAATATGAAAAGGTAAGTGCTGAATCTATTAGTGATGAAGATAGTATGAATCATACTTATCAACCAACTTCTGATTTTACAACAGCAAATATCGATAATTTAACAAATATGGTATTTAGTGCTCGGGTTATGAATGGTGAAATTTATTTATATGAAACGAAACCAAAAGTTATTCCTTTCTTAAAATATGTTTTTTTTGCTGTTAGTTTAGCTTTGGTTTTTTTTACAACATTAACGTATGCTTTTCTAATGGATTTTGGCACACATATGTCAAGTGTTATTAATGTTAATGGTATTACTCATATTGTTCGTTTAGTTTCGCCAACATTTCCAACCCAGTTAGTGATATCAATATTCATTATGATTTATGCTGGAATAAATGCTTTTAAAAGAAAAATTAGTGAAAATGAGAAGTTTCATTTTAAAAATTTTATTTGA", "aa": "MGFYTPRNQENNTSTEYEKVSAESISDEDSMNHTYQPTSDFTTANIDNLTNMVFSARVMNGEIYLYETKPKVIPFLKYVFFAVSLALVFFTTLTYAFLMDFGTHMSSVINVNGITHIVRLVSPTFPTQLVISIFIMIYAGINAFKRKISENEKFHFKNFI", "aa_hexdigest": "fd124882352c52d83ad91ce32150efdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18378.8515, "isoelectric_point": 6.053091239929199 }, "id": "GPKLONFDEH_659", "locus": "GCA000006625_00636" }, { "type": "cds", "sequence": "contig_1", "start": 375661, "stop": 376047, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGAGCATTATTAGTAGTTGCATTAGCAAATATTTTTACTGGAAATTTAAGATTTTCAAGTAGTTCATTTGATGAAATGTTTCGTCAAGCACAAATTAATGATGTATTAGCTAACTTAGCAAGAAATAATAGTTTTAGTTTTGATTATTCGTATTTACAAAGTATTTTTTATGGCTATAGTATCGCCCAAATTTTAGTATTTGTAGTTATTATTTTAGGAACAATTTTAATTATTAGTACTTTTATTTTAAATCCTAAACGTGATATTCAAAGAATAAAATTATTATTACAAGAAATTCATGATGATATTTTAACAGGTAAAATTGATCCTAATACATATTTAAGAACAAAAAATCCCTTTAGAGACTTGTTTGGTTTTTAG", "aa": "MFGALLVVALANIFTGNLRFSSSSFDEMFRQAQINDVLANLARNNSFSFDYSYLQSIFYGYSIAQILVFVVIILGTILIISTFILNPKRDIQRIKLLLQEIHDDILTGKIDPNTYLRTKNPFRDLFGF", "aa_hexdigest": "eee3a781a1bdf064dcd012e7ab8623c3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14664.868799999975, "isoelectric_point": 8.028650856018068 }, "id": "GPKLONFDEH_660", "locus": "GCA000006625_00637" }, { "type": "cds", "sequence": "contig_1", "start": 376048, "stop": 376302, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCAAGATTTAAATTTAACTATGATATAAATAATAAATTAGCATTATCCTTTACAAAGGATTTAATTGCTAATAAAAAGCAAAAAAATAAAAAAAAATTTTTGCTTTTTTTATTTCCCTTTTATCCTTTAAAACTTTTTAAAACGATTAATTTAATCGTGGTTTTTGCTGTTTTAGTTGCAATTCGTTTAGGAATGGGATTTTTACATATTAAAATTCCTGGTTTTAATATGGTTATTTCTTTTACATGA", "aa": "MNSRFKFNYDINNKLALSFTKDLIANKKQKNKKKFLLFLFPFYPLKLFKTINLIVVFAVLVAIRLGMGFLHIKIPGFNMVISFT", "aa_hexdigest": "62db853b511d16d04ca22ba8fc0cbb54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9832.900099999992, "isoelectric_point": 10.551749992370606 }, "id": "GPKLONFDEH_661", "locus": "GCA000006625_00638" }, { "type": "cds", "sequence": "contig_1", "start": 376411, "stop": 376641, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCTATTCAAGAACCTATTGTAGGCTTTATTGCTGGATTTCTAGGATCTGTAGCTACATTAAGAAAAAAAACTAAACGACCAATAATTGATCTAATAGTTAATCAAATCTTTATTTTATTTTTTAGTATATTAACATTGTTTATGATTTTGACCTTAGTTAATGATGGTAAAAACTCCTTATTTAGCATTCTTAAAAAACGTGATAAAGAAATTAATGAAGATTGA", "aa": "MYAIQEPIVGFIAGFLGSVATLRKKTKRPIIDLIVNQIFILFFSILTLFMILTLVNDGKNSLFSILKKRDKEINED", "aa_hexdigest": "7123f0a9ecae0af4597d74d6d8c99d38", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8674.305999999993, "isoelectric_point": 9.520900535583493 }, "id": "GPKLONFDEH_662", "locus": "GCA000006625_00639" }, { "type": "cds", "sequence": "contig_1", "start": 376690, "stop": 377022, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGTTGAAGCTTTTGTTATCATTTATTATTTACGTAATAGAAATCGTTCAAAAGTACAGTTAATTAATTTTATTTATTGCTCATTATTAGTAATTTTTTCAACAATGTTATTTTCGTTTGCACTAGGACCTTACGTAAGTGTAGCGTATTTAAAATATATAAAAGTCCCAATTACGAATTATTTAAAATACGGAGTTAATTACTATTTATTACCACGCATTATTAAAGAAAGCATTAAAACACCAATTTATATCATTTTATTATCTTCAATAATTAGTGCATTTGATTATGTCTTTCTAAATATTAAGCAAATTAATATGCACAAATGA", "aa": "MLVEAFVIIYYLRNRNRSKVQLINFIYCSLLVIFSTMLFSFALGPYVSVAYLKYIKVPITNYLKYGVNYYLLPRIIKESIKTPIYIILLSSIISAFDYVFLNIKQINMHK", "aa_hexdigest": "5ddd7168fabfa81d7b7e657b4907d71f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12992.540699999985, "isoelectric_point": 9.743767738342285 }, "id": "GPKLONFDEH_663", "locus": "GCA000006625_00640" }, { "type": "cds", "sequence": "contig_1", "start": 377760, "stop": 377876, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACCCTAAAAATAAAGGTTATTATTTTTTAGTTGATAAATACACAAATACAATTATTTCTTACACTAAACGAACTAATAAAAATGATTTTTACCATATTAAAAAAATTAAATAA", "aa": "MDPKNKGYYFLVDKYTNTIISYTKRTNKNDFYHIKKIK", "aa_hexdigest": "e711bf0ab10ee7e4ef3530092c3256a5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4690.3793000000005, "isoelectric_point": 9.795277976989745 }, "id": "GPKLONFDEH_664", "locus": "GCA000006625_00641" }, { "type": "cds", "sequence": "contig_1", "start": 378226, "stop": 378501, "strand": "-", "frame": 1, "gene": "udk", "product": "uridine kinase", "db_xrefs": [ "COG:COG0572", "COG:F", "EC:2.7.1.48", "GO:0004849", "GO:0005524", "GO:0005737", "GO:0043771", "GO:0044206", "GO:0044211", "KEGG:K00876", "SO:0001217", "UniRef:UniRef50_Q9PQF9", "UniRef:UniRef90_Q9PQF9" ], "nt": "ATGCGAGAGCAATTAAGTGACATTAAAAAACGTAAAAAAATTAAAGTACCAATCTATGATTATAAAACTGAGATTCGTTTAGATAAAACGATTGATATTAGTGATGTTGATGTTATAGTTCTTGAAGGTATTTATGCAATTTATGATGATGTCATTAATCAAATAGCTGATTTGAAAATTTTTATTGAAACTCCAAAAGATGAATGTTTAATTCGACGAATTCTTCGAGATGTTAATGAACGTAATCGATCATTTGAATCTGTAATTACTCAATGA", "aa": "MREQLSDIKKRKKIKVPIYDYKTEIRLDKTIDISDVDVIVLEGIYAIYDDVINQIADLKIFIETPKDECLIRRILRDVNERNRSFESVITQ", "aa_hexdigest": "45b2f025b49765d14a63862a862ba196", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "udk" ], "truncated": "both", "pseudogene": { "start": 378091, "stop": 378714, "observations": { "mutation": [ 378111, 378153, 378228, 378510 ], "stop": [ 378111, 378153, 378228, 378510 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQF9", "query_cov": 1.0, "subject_cov": 0.4396135265700483, "identity": 1.0, "score": 171.0, "evalue": 3.02e-52, "gene_start": 1, "gene_end": 91, "reference_start": 72, "reference_end": 162, "reference_sequence": "MNTKSPILILIAGASGSGKTTFANEIIARIPKNTTSVVICQDSYYISNSQLNKKERRLINYDHPSSFEWELMREQLSDIKKRKKIKVPIYDYKTEIRLDKTIDISDVDVIVLEGIYAIYDDVINQIADLKIFIETPKDECLIRRILRDVNERNRSFESVITQWRSTVSPMYDQFVEPSKKNANVSVLWNEHNRVALHLIHKWINNIH" }, "paralog": false, "identity": 0.9806763285024155, "score": 393.0, "evalue": 2.38e-142, "description": "Internal stop codon at 378111, 378153, 378228, 378510. Nonsense mutation around 378111, 378153, 378228, 378510.", "psc": { "uniref90_id": "UniRef90_Q9PQF9", "gene": "udk", "product": "uridine kinase", "ec_ids": [ "2.7.1.48" ], "uniref50_id": "UniRef50_Q9PQF9", "cog_id": "COG0572", "cog_category": "F", "kegg_orthology_id": "K00876", "go_ids": [ "GO:0004849", "GO:0005524", "GO:0005737", "GO:0043771", "GO:0044206", "GO:0044211" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQF9", "EC:2.7.1.48", "UniRef:UniRef50_Q9PQF9", "COG:COG0572", "COG:F", "KEGG:K00876", "GO:0004849", "GO:0005524", "GO:0005737", "GO:0043771", "GO:0044206", "GO:0044211" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQF9" ], "product": "Uridine kinase" } }, "id": "GPKLONFDEH_665", "locus": "GCA000006625_00642" }, { "type": "cds", "sequence": "contig_1", "start": 378508, "stop": 378714, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07693.20", "PFAM:PF00485.24", "PFAM:PF06414.18" ], "nt": "ATGAATACTAAATCCCCAATTCTTATACTAATTGCAGGAGCTTCTGGTTCTGGAAAAACAACCTTTGCTAATGAAATTATTGCAAGAATTCCTAAAAATACAACAAGTGTTGTTATTTGTCAAGATTCTTACTATATTTCTAATTCACAACTAAATAAAAAAGAACGCCGCTTAATTAATTATGATCATCCAAGTAGTTTTGAATGA", "aa": "MNTKSPILILIAGASGSGKTTFANEIIARIPKNTTSVVICQDSYYISNSQLNKKERRLINYDHPSSFE", "aa_hexdigest": "c8804014d727ea3dd4bc388c8bece399", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07693.20", "name": "KAP_NTPase", "length": 45, "aa_cov": 0.6617647058823529, "hmm_cov": 1.0, "evalue": 1.2731880507614692e-05, "score": 20.196245193481445, "start": 3, "stop": 47 }, { "id": "PF00485.24", "name": "PRK", "length": 46, "aa_cov": 0.6617647058823529, "hmm_cov": 1.0, "evalue": 5.133078498870733e-06, "score": 22.001033782958984, "start": 9, "stop": 53 }, { "id": "PF06414.18", "name": "Zeta_toxin", "length": 42, "aa_cov": 0.6176470588235294, "hmm_cov": 1.0, "evalue": 1.6067081083008945e-07, "score": 26.538028717041016, "start": 3, "stop": 44 } ], "seq_stats": { "molecular_weight": 7546.507200000001, "isoelectric_point": 9.300869941711426 }, "id": "GPKLONFDEH_666", "locus": "GCA000006625_00643" }, { "type": "cds", "sequence": "contig_1", "start": 378900, "stop": 379073, "strand": "+", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_006688693.1", "SO:0001217", "UniParc:UPI0000165FAF", "UniRef:UniRef100_Q9PQF8", "UniRef:UniRef50_Q9PQF8", "UniRef:UniRef90_Q9PQF8" ], "nt": "ATGGCTGTACAACAAAGAAGAGTAAGTAAATCAAGAAAAGGAATGCGTCGTAGTCACGATCACCTTACAATTTCTAATACAGTAGCATGTAATGAATGTGGTAAAGCATTACTACCTCATAGAGCTTGTCGTGATTGCAAAACTTACAGAAGTATTAAGTTATCAATCAAATAA", "aa": "MAVQQRRVSKSRKGMRRSHDHLTISNTVACNECGKALLPHRACRDCKTYRSIKLSIK", "aa_hexdigest": "6a0937f7e2a46bfe60dcf907b1e2e355", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000165FAF", "ncbi_nrp_id": "WP_006688693.1", "uniref100_id": "UniRef100_Q9PQF8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165FAF", "RefSeq:WP_006688693.1", "UniRef:UniRef100_Q9PQF8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQF8", "uniref90_id": "UniRef90_Q9PQF8" }, "psc": { "uniref90_id": "UniRef90_Q9PQF8", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_Q9PQF8", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQF8" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "GPKLONFDEH_667", "locus": "GCA000006625_00644" }, { "type": "cds", "sequence": "contig_1", "start": 379082, "stop": 379339, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688677.1", "SO:0001217", "UniParc:UPI000165F8C3", "UniRef:UniRef100_A0AAC9X6U7", "UniRef:UniRef50_A0AAC9X6U7", "UniRef:UniRef90_A0AAC9X6U7" ], "nt": "ATGAATGATAGTGTTAAACAAACCAATAATATTGATGAAAATCAATTAAAACCAACTACAAAAAGCTTTTTTGATGATAAAAACAACCAATTAACAAAAGTTTTAAATATTCCTAGCGTTGTTAAATATACGGAATCAAAAATTAAAAAAAATGGTAAAAATTTTTCAATTAATTTAATCATTACAATTACAACAATGTTTATTGTAATTGGGATTATTATAACTATTGCTATTGCTATTGGTATTAAATTAAGTTAG", "aa": "MNDSVKQTNNIDENQLKPTTKSFFDDKNNQLTKVLNIPSVVKYTESKIKKNGKNFSINLIITITTMFIVIGIIITIAIAIGIKLS", "aa_hexdigest": "87b2722beb4702ccb8a3049dac005790", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8C3", "ncbi_nrp_id": "WP_006688677.1", "uniref100_id": "UniRef100_A0AAC9X6U7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8C3", "RefSeq:WP_006688677.1", "UniRef:UniRef100_A0AAC9X6U7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6U7", "uniref90_id": "UniRef90_A0AAC9X6U7" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X6U7", "uniref50_id": "UniRef50_A0AAC9X6U7" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6U7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6U7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9498.065799999991, "isoelectric_point": 9.699284553527832 }, "id": "GPKLONFDEH_668", "locus": "GCA000006625_00645" }, { "type": "cds", "sequence": "contig_1", "start": 379425, "stop": 379787, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATATTAATTTAAGGGTGAAAACAATGATGGAAAAATTAAAAAACAACAAGACTTTGTTTTTGAATGTAGAACAAGCTCAAAAACTAGGGATTAATAACCCTTTAACACAAGAGATTTTTTTAGATGATCAAAAGTTAAAAGTTCATTTAATTAATAGTATAAAAGATGAGTTTTACTTAAAAAAAAGTATTAGTAAAGGTTATGCTTATTTTAATAGTTTAGAACAATTTAAGACTATGGAGCTTGATGTTTTAGATAACTATCAATTTGCAAAAATAGCAGCTACTAATTTAGATGAGTATGATCAATTAGTTTACACAAAAACTCGTCAATATTTAATTAATAAAGAATTTTGA", "aa": "MFYINLRVKTMMEKLKNNKTLFLNVEQAQKLGINNPLTQEIFLDDQKLKVHLINSIKDEFYLKKSISKGYAYFNSLEQFKTMELDVLDNYQFAKIAATNLDEYDQLVYTKTRQYLINKEF", "aa_hexdigest": "cc1f27f7b6ef7a65585ca6fc745f99e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14350.437699999984, "isoelectric_point": 8.667854881286619 }, "id": "GPKLONFDEH_669", "locus": "GCA000006625_00646" }, { "type": "cds", "sequence": "contig_1", "start": 379918, "stop": 380049, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATCTTAAATAAATTAAAATTTGAAGAATATCTAAATTTGATTCAAGGGTTGCTTTTTTATCATAAAATTTCTCATAATCTAATTTATTTTGACCATGAATACTTAAATTATGAATTTGTTTACTAA", "aa": "MKILNKLKFEEYLNLIQGLLFYHKISHNLIYFDHEYLNYEFVY", "aa_hexdigest": "767510cd1581da8a0e44cba2ca33adba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5458.286599999999, "isoelectric_point": 6.233384513854981 }, "id": "GPKLONFDEH_670", "locus": "GCA000006625_00647" }, { "type": "cds", "sequence": "contig_1", "start": 380136, "stop": 381446, "strand": "+", "frame": 3, "gene": null, "product": "DUF31 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0W6", "UniRef:UniRef90_A0AAC9T0W6" ], "nt": "TTGCCGTTTTCATTTCAACGTTATGATTATGAATCAAGACGTAATTTCATTAGAAACTCACGTCAATTAGTTGATACCTCAATTGATTTTGACGATCTTGAATATGATTTTTTGCACAATAAGAAACCAAAACAAGAACTAGAAAAAAATAATGAATCAATTAAAATAGCTGAATTAAAAAAATTTGAAAATTCACAATTAAGTGATGAACAACAATCTAATTTAGAAAAAGAGTTCTATAGTATTGGAAGAATTAACTGTTTTGACGAAGTTAGCGAAAATAAACAAGAATTAAACAAAGAACAATTAGTAATCGATAAAAAAAATGGGTATTTGAAGCCACGTAGTTACTTACGTCATCATGAAACTCCATTTATGGTTGACATTGAGAACCGGGTAGACTTTTTAATGGCAGGATATGAAATTATTAAAAATGATGATCCATATGCAATTAAACAAGAAATATTAAAAAATACCATTTTAGAACAACAAAAGCTTGTCGAACAAGATGATCATGATTTTAAAGTTAAAAATGATGATCAAGAAGCAAATGAAGAATTATTGGACAATACTGATAAATTAGAAAATCAAGATTCTACAAGTGCTGTTGAATTTAATGATTCATCTGAAGTTACTCAAGTAGTAAAAACGGATGAAAATGTTGATAATTTAATTAAAACTAAGCAAATAACTAGTTTTAACGATCTTAAACAAGAGCAATCATCTCAAGAAAATATTATTAAAAATGATCTTTCATCAATTCAAATTCGTTTAGATGAAATTATTAATGTACATGAAAAAGATACTAAATTACAAACCGAACTAAATTCAATTATGATAGATAGTAATGAAAAAAATATTGATGATAGTTTAAATAATTTAAATGAACAAATACGAATTCAAGATCAAGAATTAGAATCATCAGATAATCAAAATGTAATTATTGAAGAAGAAATTAATACCAAAAATTTAAATAATAAAGCTGATGTTGGTGTTATGAGTGTTGAAAAAAAATATGAACCTGAAGAGGAAATGGTTAATTTTGAATTTGAAATACCTGAAAACCAAAAAGAATCAGTTAACCAAGATGTTTTAAATAGTAATAGTATTGATAATTTTAAAGAAGAAAAAGCTAAAGAAAATACTTTCTTAACACCAAAAGAAATAGAAAATAATGATTTATCATCACAAATTGAATTAACACCTGCTGATTTTGAACTTAAGACACAAGAAATTAATGACGAAACTGAACGTTTATTGGATGAATTAAATAATAATAAACCTAAAGAAAAGAAGAAATTTTGGACATGA", "aa": "MPFSFQRYDYESRRNFIRNSRQLVDTSIDFDDLEYDFLHNKKPKQELEKNNESIKIAELKKFENSQLSDEQQSNLEKEFYSIGRINCFDEVSENKQELNKEQLVIDKKNGYLKPRSYLRHHETPFMVDIENRVDFLMAGYEIIKNDDPYAIKQEILKNTILEQQKLVEQDDHDFKVKNDDQEANEELLDNTDKLENQDSTSAVEFNDSSEVTQVVKTDENVDNLIKTKQITSFNDLKQEQSSQENIIKNDLSSIQIRLDEIINVHEKDTKLQTELNSIMIDSNEKNIDDSLNNLNEQIRIQDQELESSDNQNVIIEEEINTKNLNNKADVGVMSVEKKYEPEEEMVNFEFEIPENQKESVNQDVLNSNSIDNFKEEKAKENTFLTPKEIENNDLSSQIELTPADFELKTQEINDETERLLDELNNNKPKEKKKFWT", "aa_hexdigest": "dfc5b22a0306bf60b346521c5a82b96b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 379836, "stop": 381467, "observations": { "mutation": [ 379884, 381444 ], "stop": [ 379884, 381444 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0W6", "query_cov": 1.0, "subject_cov": 0.6411764705882353, "identity": 0.998, "score": 803.0, "evalue": 8.91e-289, "gene_start": 1, "gene_end": 436, "reference_start": 238, "reference_end": 673, "reference_sequence": "MFYINLRVKTMMEKLKNNKTLFLNVEQAQKLGINNPLTQEIFLDDQKLKVHLINSIKDEFYLKKSISKGYAYFNSLEQFKTMELDVLDNYQFAKIAATNLDEYDQLVYTKTRQYLINKEFWTNDGLDAFIGDDFKRKQLTSKEIVNLKTKYDLWIQELHFNNARISKQIHNLSIHGQNKLDYEKFYDKKATLESNLDILQILIYLRSSLLELDFDDLFKDDQNKISIRQLLVNDLEYLPFSFQRYDYESRRNFIRNSRQLVDTSIDFDDLEYDFLHNKKPKQELEKNNESIKIAELKKFENSQLSDEQQSNLEKEFYSIGRINCFDEVSENKQELNKEQLVIDKKNGYLKPRSYLRHHETPFMVDIENRVDFLMAGYEIIKNDDPYAIKQEILKNTILEQQKLVEQDDHDFKVKNDDQEANEELLDNTDKLENQDSTSAVEFNDSSEVTQVVKTDENVDNLIKTKQITSFNDLKQEQSSQENIIKNDLSSIQIRLDEIINVHEKDTKLQTELNSIMIDSNEKNIDDSLNNLNEQIRIQDQELESSDNQNVIIEEEINTKNLNNKADVGVMSVEKKYEPEEEMVNFEFEIPENQKESVNQDVLNSNSIDNFKEEKAKENTFLTPKEIENNDLSSQIELTPADFELKTQEINDETERLLDELNNNKPKEKKKFWTWFNSKKR" }, "paralog": false, "identity": 0.996316758747698, "score": 1062.0, "evalue": 0.0, "description": "Internal stop codon at 379884, 381444. Nonsense mutation around 379884, 381444.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0W6", "product": "DUF31 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0W6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0W6", "UniRef:UniRef50_A0AAC9T0W6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0W6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0W6" ], "product": "DUF31 domain-containing protein" } }, "id": "GPKLONFDEH_671", "locus": "GCA000006625_00648" }, { "type": "cds", "sequence": "contig_1", "start": 381491, "stop": 381907, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAACTAATCAAATTGTGGATTTATTAACCAATAAACAAAATAATTCAATTCAAAGACGCGATAATCTAGTTTTTGTTAAAAATGAACAAGAATTCAAAGATATTTTAGATTCTACTATTAGCTTAAGTAATGTTTATGATCCTTTGTTAGGTAATGGTTGTGAACAAGAAAAAATAAAAGAATTAACTATTTTAAATAATAGTGTTGATATTAGCAAAATTGAAGAGTATACTAAAACATTAGAAAGTAAAGTAGATGATTTATACAATAATGTTTCTAACCAACTAATTCAAGATTATCAAAAAAATGAGAATACAAGTGCGAATAATATAATTATGAAAAATGAAGAATTATTAAATTCTCATTTAAAAGAAGTCGAAAAAATTTGTGAACAGCTAATTGATGAATGA", "aa": "MNKTNQIVDLLTNKQNNSIQRRDNLVFVKNEQEFKDILDSTISLSNVYDPLLGNGCEQEKIKELTILNNSVDISKIEEYTKTLESKVDDLYNNVSNQLIQDYQKNENTSANNIIMKNEELLNSHLKEVEKICEQLIDE", "aa_hexdigest": "6db41be1dbdb4d8fcd24e404faae96b1", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16007.697299999994, "isoelectric_point": 4.596762657165528 }, "id": "GPKLONFDEH_672", "locus": "GCA000006625_00649" }, { "type": "cds", "sequence": "contig_1", "start": 381986, "stop": 382192, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGTAAAATCATTAATCAATGAAGATAGTTTTTTAACAAAAAGACAAGTTAAAAACAAAAATAAAATTCTTACTGAATTTGAAAAACATCGTTCACAAATTACACAAAATTATTTAAATAAACGTAGTGATATTCTCAAGAAAGAATATGATGAATTATTAAAAGATGATAAATTATTTGAGTTAAGCTCAGGCGATAATTGA", "aa": "MKVKSLINEDSFLTKRQVKNKNKILTEFEKHRSQITQNYLNKRSDILKKEYDELLKDDKLFELSSGDN", "aa_hexdigest": "60a422ef7354573f8436d632154b2f43", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8147.1923, "isoelectric_point": 9.222282981872556 }, "id": "GPKLONFDEH_673", "locus": "GCA000006625_00650" }, { "type": "cds", "sequence": "contig_1", "start": 382370, "stop": 382798, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGATTTACGTGTTAAATTTATGTATGGTTTAGGTTCAGAAATTGTTATTTTAAAATTTTTAATTGAGCATTATAAAAAATTAAATAGTTTACGTTTAAGATTAGAAACATTAAACCACCCTCTTTCAAATTATGCTGTTTATTTTGATATTTTAAACCGAATTGAAAAACAAACACAATTGTTAGCAAAAATTGAAACTTTAAAATATGATAATTATGATTTTAATGCTGATGTTATTTTAGATGATTTAGAAAGTAATATCACAAAAATAAATGAGATTAAACATAATTCTAATGTTTTAAATAAAACAAAAGTTATTAAAACGATTATTGAGCCAAAATATGAATTAGAATTAATTTCAAAAGATGATAAAGTTGATGGTGTTAAGGTTTACGTTAACGATCAAGAAATTCTTTCTAAATAA", "aa": "MYDLRVKFMYGLGSEIVILKFLIEHYKKLNSLRLRLETLNHPLSNYAVYFDILNRIEKQTQLLAKIETLKYDNYDFNADVILDDLESNITKINEIKHNSNVLNKTKVIKTIIEPKYELELISKDDKVDGVKVYVNDQEILSK", "aa_hexdigest": "6e2a042f58e862089ebddd98717f24f2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16739.193799999986, "isoelectric_point": 6.306877326965333 }, "id": "GPKLONFDEH_674", "locus": "GCA000006625_00651" }, { "type": "cds", "sequence": "contig_1", "start": 382856, "stop": 383665, "strand": "+", "frame": 2, "gene": "folD", "product": "methenyltetrahydrofolate cyclohydrolase", "db_xrefs": [ "COG:COG0190", "COG:H", "RefSeq:WP_006688679.1", "SO:0001217", "UniParc:UPI00000C1C25", "UniRef:UniRef100_Q9PQF4", "UniRef:UniRef50_Q9PQF4", "UniRef:UniRef90_Q9PQF4" ], "nt": "ATGAAACTAAAAATTGATTTTTTATACGAAAAAGTCTTAACAGAATTATTAACTAAAAAAAACAAAACTAAAAAACGTATTTATGTTTTTTATGATTCAGATTTAGTAAATTTTAATAGTGCATATATTCAATCAAAATTAAAATATGCTAAATTATTTTCTGTTAAACTTATTATTTATGATTTAAATGCATACCAAATTCAAGAAAATCATCATTTTTTATCAAAACTTAAACAAGCAAATAAGGATTTTTTGTTTTTTGAATTCCCTTTGTCAAAAAAACATTTGGATCAAAATTATTTTCAATATTTAACTTTTCAAAATGATCTCGATGGAATTAATTCACATTTGTTTTTAAATAAAAAACAAGAAAATCAAATTATTTACCATCCAGCAACAATTAATGCAATTTTAACATTAATTAAAGAATTAGATGTAAATTTAAATGACTATCGAATTTGCATTATTGGTCGTTCACCCTATTTAGGGGATTATTTATATAAGTTATTAAATCCTAAAAATAATAAAATTAATATTATTCATTCACAAACTAAAAATCCATTAAGAATTATTAAAGAATCTAATTTTATTATTAGCTGTGTGAATAAAGCACACATTTTTAATTCTAATGCATTAAGTGATAATTCTTATTTAATTGATGTTACTACAGAATACATCAATAATAAGTTATGTGGTTCTTTTTTAATTAATAAAAATGATGCATTAAATCGAAACATTAAATACACTCCTGTACCAGGTGGAATCGGTAAATTAACGACGTTATTTTTATTTTTGAATTATTTTAAATAA", "aa": "MKLKIDFLYEKVLTELLTKKNKTKKRIYVFYDSDLVNFNSAYIQSKLKYAKLFSVKLIIYDLNAYQIQENHHFLSKLKQANKDFLFFEFPLSKKHLDQNYFQYLTFQNDLDGINSHLFLNKKQENQIIYHPATINAILTLIKELDVNLNDYRICIIGRSPYLGDYLYKLLNPKNNKINIIHSQTKNPLRIIKESNFIISCVNKAHIFNSNALSDNSYLIDVTTEYINNKLCGSFLINKNDALNRNIKYTPVPGGIGKLTTLFLFLNYFK", "aa_hexdigest": "ab997608c4967d36cd2413dd58cc9ac7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C25", "ncbi_nrp_id": "WP_006688679.1", "uniref100_id": "UniRef100_Q9PQF4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C25", "RefSeq:WP_006688679.1", "UniRef:UniRef100_Q9PQF4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQF4", "uniref90_id": "UniRef90_Q9PQF4" }, "psc": { "uniref90_id": "UniRef90_Q9PQF4", "gene": "FolD", "product": "methenyltetrahydrofolate cyclohydrolase", "uniref50_id": "UniRef50_Q9PQF4", "cog_id": "COG0190", "cog_category": "H" }, "pscc": { "uniref50_id": "UniRef50_Q9PQF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQF4" ], "product": "methenyltetrahydrofolate cyclohydrolase" }, "genes": [ "folD" ], "id": "GPKLONFDEH_675", "locus": "GCA000006625_00652" }, { "type": "cds", "sequence": "contig_1", "start": 383742, "stop": 384953, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBE8", "UniRef:UniRef90_A0A2C9DXX8" ], "nt": "ATGATTGACAAGCAAAAAATTAAACAACTAATCCAAAATCCTGTTTATGGATTTTTTGATGAATTTAAAAAAAATTTAAACAGCGATCTTGATCATGTAATTTTAGAACAATTAAAAGATCAAGATAATAATATTGATAAAATTAATCATTTAGTAACCGATATTAATAATTTAAAAAATGATATTAAACAACGCAAAAAAGATGAAAAAATTAAACAACGGACTTTTTGTAATATTCTAATCGCAGTTTGTTTTGTTATTATTATTGGTTTGTTTTTTTTACATTTTTATTTGAAAAATTCCAAATTTATTAAAAATTTTAAAAAGTATGAAATTGAAAAAAATAATCAAATTAATATATGTAACAGCCAAAAAAATAAATATATTTATAATGTTTTATCACAAATTAATTCTTATAAAATTTCGCAAGAAATTTTTGCTAAGAATGGTTTATATTTAATGCCAGAAATTCAATATGAAAATATTAAACAAACCGATTTATTAATTTCACGAAACGCAAAATTTGTTGGATTTTTTGGTGGTTTAGAAGTTAAATTTAAAAGTACGGATACTTTTTTAGTGTATTATAAGGAATTTTCAATTAAAGATGTTGTAACAAGTGGTTGTATTCAAATTTCTTATCGAAAAGGAGATCAAATGGTTAGTGAAACGATCGTTGCTCATCATAAGGAACCAACACCGTTTGTTGATCTTCACAGTAATTTTGTTCATAAAACTAATTACTCTCCAAACTTTAACTTTTCAACGTACGCTCAAACTGTTAATTCTCGTTCAAAAACAATTTTTTCTAATTTAGAATTTTCTAAGTATTATGGCTTAGGAATTGCTACTCAAAATTTAGAATTTGATACAAAAATGCTAGAATTTTTTACGCCTTTTAGTCAAGAAAACTATGAAAATTTTGCTAAATATTTAAAACAAGATGATATCACAGTTCCTATGTTTACAAAAACTGCGACATCCCTTATTATTGATGATCAATTATTAAAAGATAATAGAAAACCATTCTTTATAAATTTATTAACAAATAATAGTTATGTAAAAACTTGTGATTTTCTAATTGATACAGATATTAATGCGGACCTACAAACGAATTTAAATATTTTAAAATATGAATTAGTAAAATTATTTGAAAAATATATTTTATATTTAACTACAACAAGTTTATCAAGTGTAATTGCCCGAGAATGA", "aa": "MIDKQKIKQLIQNPVYGFFDEFKKNLNSDLDHVILEQLKDQDNNIDKINHLVTDINNLKNDIKQRKKDEKIKQRTFCNILIAVCFVIIIGLFFLHFYLKNSKFIKNFKKYEIEKNNQINICNSQKNKYIYNVLSQINSYKISQEIFAKNGLYLMPEIQYENIKQTDLLISRNAKFVGFFGGLEVKFKSTDTFLVYYKEFSIKDVVTSGCIQISYRKGDQMVSETIVAHHKEPTPFVDLHSNFVHKTNYSPNFNFSTYAQTVNSRSKTIFSNLEFSKYYGLGIATQNLEFDTKMLEFFTPFSQENYENFAKYLKQDDITVPMFTKTATSLIIDDQLLKDNRKPFFINLLTNNSYVKTCDFLIDTDINADLQTNLNILKYELVKLFEKYILYLTTTSLSSVIARE", "aa_hexdigest": "366fc645c38ab254ee94c3afaa4e9de7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 383742, "stop": 385256, "observations": { "mutation": [ 384951 ], "stop": [ 384951 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DXX8", "query_cov": 1.0, "subject_cov": 0.64171974522293, "identity": 1.0, "score": 755.0, "evalue": 3.77e-271, "gene_start": 1, "gene_end": 403, "reference_start": 1, "reference_end": 403, "reference_sequence": "MIDKQKIKQLIQNPVYGFFDEFKKNLNSDLDHVILEQLKDQDNNIDKINHLVTDINNLKNDIKQRKKDEKIKQRTFCNILIAVCFVIIIGLFFLHFYLKNSKFIKNFKKYEIEKNNQINICNSQKNKYIYNVLSQINSYKISQEIFAKNGLYLMPEIQYENIKQTDLLISRNAKFVGFFGGLEVKFKSTDTFLVYYKEFSIKDVVTSGCIQISYRKGDQMVSETIVAHHKEPTPFVDLHSNFVHKTNYSPNFNFSTYAQTVNSRSKTIFSNLEFSKYYGLGIATQNLEFDTKMLEFFTPFSQENYENFAKYLKQDDITVPMFTKTATSLIIDDQLLKDNRKPFFINLLTNNSYVKTCDFLIDTDINADLQTNLNILKYELVKLFEKYILYLTTTSLSSVIAREWYINNDTYKIGDNYDYESYYYTNQNQLTPLSVSTKLFLKNQIAFVNDCEVIPFAELVNNQTRFGINKAQFNIKSYHVYNRVDNVPVYAHGRTHIVPVQYKEYIEYEYPFICLYVLKYKQLTEEIGLKYFTRPFNNILLTNFEDHSACSIDEILDFNDNESKNNSDNDFKIYLKELDTIVTNANLNKNEFSFLKDNDGYFITISNKIELDEETEQELSTWLRKVSL" }, "paralog": false, "identity": 0.998015873015873, "score": 983.0, "evalue": 0.0, "description": "Internal stop codon at 384951. Nonsense mutation around 384951.", "psc": { "uniref90_id": "UniRef90_A0A2C9DXX8", "uniref50_id": "UniRef50_B5ZBE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DXX8", "UniRef:UniRef50_B5ZBE8" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 47324.77590000002, "isoelectric_point": 8.726843452453611 }, "id": "GPKLONFDEH_676", "locus": "GCA000006625_00653" }, { "type": "cds", "sequence": "contig_1", "start": 385804, "stop": 386415, "strand": "+", "frame": 1, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "nt": "ATGGCAAAAGAAATTATTTTAGGAATTGATTTGGGTACAACAAATTCATGTGTAGCCGTAATTGAAAATAAAAAACCAATTGTTTTAGAGAATCCAGAAGGAAAACGAACAGTTCCATCTGTAGTTTCATTTAACGGCGATGAAGTTTTAGTAGGTGATGCTGCAAAACGTAAACAAATAACTAATCCAAATACTATTTCATCAATCAAACGTTTAATGGGTACAAAAGAAAAAGTAACTGTTTTAAATAAAGATTATACTCCAGAAGAGATTTCAGCAAAAATTTTAACTTATATTAAAGAGTATGCAGAAAAGAAAATTGGAGCAAAAGTTAATAAGGCTGTAATTACAGTTCCAGCATATTTTGATGATGCACAACGTCAAGCTACAAAAAATGCAGGAATTATTGCAGGATTGAGCGTTGAAAGAATTATTAATGAACCAACAGCTGCAGCTTTAGCTTATGGAATTGATAAATTAGATAAAGAACAAAAAATATTGGTATTTGATTTAGGTGGTGGTACATTTGATGTATCAGTTTTAGATATGGCAGATGGTACATTTGAAGTATTATCAACAAGTGGTGATAACCATTTAGGTGGTGATGATTGA", "aa": "MAKEIILGIDLGTTNSCVAVIENKKPIVLENPEGKRTVPSVVSFNGDEVLVGDAAKRKQITNPNTISSIKRLMGTKEKVTVLNKDYTPEEISAKILTYIKEYAEKKIGAKVNKAVITVPAYFDDAQRQATKNAGIIAGLSVERIINEPTAAALAYGIDKLDKEQKILVFDLGGGTFDVSVLDMADGTFEVLSTSGDNHLGGDD", "aa_hexdigest": "6610a457ef2d51b84259f29e0a80181a", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_A0A7Y0YRM2", "query_cov": 0.9753694581280788, "subject_cov": 0.8181818181818182, "identity": 0.6970000000000001, "score": 275.0, "evalue": 5.42e-91, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_W7C0L0" }, "pscc": { "uniref50_id": "UniRef50_W7C0L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "GPKLONFDEH_677", "locus": "GCA000006625_00654" }, { "type": "cds", "sequence": "contig_1", "start": 386488, "stop": 387615, "strand": "+", "frame": 1, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_O87777", "UniRef:UniRef90_Q9PQF2" ], "nt": "ATGGCTATGCAACGTTTAAAAGATGCTGCTGAAAAAGCTAAAATTGAATTATCAGGTATAAACACAACAACTATTTCATTACCTTTTATTGCAATGGATAGTTCTGGTCAGCCAATTAATTTTGAAAAAGAATTAAATCGAGCGACATTTGATAATCTAACTAAAAATTTAATTGAAAGATTAAAAAAACCTGTTTTAGATGCAATGAAAGAATCTAAATTATCATTGGTTGATATTGATCAAGTTTTGATGGTAGGTGGTTCAACACGTATGCCTGCAGTACAAAATTTAGTGAAAGAATTAACAGGTAAAGAACCAAACCATTCACTAAATCCTGATGAAGTTGTTGCAATTGGTGCAGCTATTCAAGGTGGAGTATTAGCAGGAGAAATTGATGATATTCTATTACTTGATGTAACACCACTTACTTTATCAATTGAAACAATGGGAGGTGTAGCAACTCCTTTAATTCCAAGAAACACAAAGATTCCAGTAAGTAAATCACAAATTTTTTCAACAGCTGCTGATAATCAACCAAGTGTTGATATAAGAATTGTGCAAGGTGAACGTTCATTAGCTGCTGATAATAAATTATTAGGTAATTTTGAATTAAGCGGAATTGAACCAGCTCCACGTGGTGTTCCACAAATTGAAATTAAGTTTAATATTGATGCTAATGGGATCATGAGTGTTAACGCTAAAGATTTAAAAACACAAAAAGAAACATCAATCACAATTAAAGATTCACAAGGATTAAGTCAAGATGAAATTGACAAAATGATTAAGGAAGCTGAGGAAAACAAAGAAAAAGATGCTAAAGTAAAACATGAACGTGAACTTGTAAATCGTGCTGATAGTCTAATTAACCAATTAGAACAAGTGTCAAAAACTGAAAATGTTCCACAAGAACAAAAAGATGTATTTAACAAACAAATTGAAGATTTAACAAACGCACGCGATGCTCAAGATTATGTAAAACTAGAAGCTGAAGTTAAAAAAGTTGAAGATTTATTAACTAATGCTGCTAAATTTGCTCAACAAGCTCAACAGCAAAATCCAGATAATCAAAACAATAATAAAGATGATGTTACTGAAGCAACTGTAACTGATGATTCAACAAAGAAATAA", "aa": "MAMQRLKDAAEKAKIELSGINTTTISLPFIAMDSSGQPINFEKELNRATFDNLTKNLIERLKKPVLDAMKESKLSLVDIDQVLMVGGSTRMPAVQNLVKELTGKEPNHSLNPDEVVAIGAAIQGGVLAGEIDDILLLDVTPLTLSIETMGGVATPLIPRNTKIPVSKSQIFSTAADNQPSVDIRIVQGERSLAADNKLLGNFELSGIEPAPRGVPQIEIKFNIDANGIMSVNAKDLKTQKETSITIKDSQGLSQDEIDKMIKEAEENKEKDAKVKHERELVNRADSLINQLEQVSKTENVPQEQKDVFNKQIEDLTNARDAQDYVKLEAEVKKVEDLLTNAAKFAQQAQQQNPDNQNNNKDDVTEATVTDDSTKK", "aa_hexdigest": "a2cf852c5ca23bc4682c8ea4d86a3e82", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6RJX7", "query_cov": 0.9706666666666667, "subject_cov": 0.9732620320855615, "identity": 0.569, "score": 365.0, "evalue": 1.25e-121, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_R6RJX7" }, "genes": [ "dnaK" ], "truncated": "both", "pseudogene": { "start": 386188, "stop": 387626, "observations": { "deletion": [ 387604 ], "mutation": [ 386413, 386434 ], "stop": [ 386413, 386434 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQF2", "query_cov": 1.0, "subject_cov": 0.6208609271523179, "identity": 0.9570000000000001, "score": 660.0, "evalue": 1.33e-234, "gene_start": 1, "gene_end": 375, "reference_start": 229, "reference_end": 600, "reference_sequence": "MAKEIILGIDLGTTNSCVAVIENKKPIVLENPEGKRTVPSVVSFNGDEVLVGDAAKRKQITNPNTVSSIKRLMGTKEKVTILNKEYTPEEISAKILSYIKDYAEKKLGTKINKAVITVPAYFDDAQRQATKNAGIIAGLTVERIINEPTAAALAYGIDKLDKEQKILVFDLGGGTFDVSVLDMADGTFEVLSTSGDNHLGGDDWDQVIINWLLKSIADEFNIDLSKNKMAMQRLKDAAEKAKIELSGVNTTTISLPFIAMDSSGQPINFEKELNRATFDNLTKNLIERLKKPVLDAMKESKLSLADIDQVLMVGGSTRMPAVQNLVKELTGKEPNHSLNPDEVVAIGAAIQGGVLAGEIDDILLLDVTPLTLSIETMGGVATPLIPRNTKIPVSKSQVFSTAADNQPSVDIRIVQGERSLAADNKLLGNFELSGIEPAPRGVPQIEIKFNIDANGIMSVNAKDLKTQKETSITIKDSQGLSQEEIDKMIKEAEENKEKDAKVKHERELVNRADSLINQLEQVVKTENVPQEQKDAFNKQIEELTNARDAQDYTKLEAEVKKVEDLLANAAKFAQQTQQQDPNNQKDDVTEATVTDDSNKKIINF" }, "paralog": false, "identity": 0.9561586638830898, "score": 868.0, "evalue": 4.8e-319, "description": "Internal stop codon at 386413, 386434. Frameshift due to deletion around 387604. Nonsense mutation around 386413, 386434.", "psc": { "uniref90_id": "UniRef90_Q9PQF2", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_O87777", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQF2", "UniRef:UniRef50_O87777", "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_O87777", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O87777" ], "product": "Chaperone protein DnaK" } }, "id": "GPKLONFDEH_678", "locus": "GCA000006625_00655" }, { "type": "cds", "sequence": "contig_1", "start": 387667, "stop": 387861, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAGTTAATATTTAATTTATGTATATATATTAAAAATAAAGGAAAATTTGAGTATATGGTAGAAAATAAATTTTTAAAGCAAATTAAAGAGGGAGAGCAAATTAAAATTGAAATTAAAGGTTTGGATAAATTTAATAATTTTGACGAACAAAATTATGGTTTAAATAATATTTCACCTCAAATTCGTTGA", "aa": "MYKLIFNLCIYIKNKGKFEYMVENKFLKQIKEGEQIKIEIKGLDKFNNFDEQNYGLNNISPQIR", "aa_hexdigest": "61d83e9273c4bb659a53a3dbe150381e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7735.952199999999, "isoelectric_point": 9.068719291687014 }, "id": "GPKLONFDEH_679", "locus": "GCA000006625_00656" }, { "type": "cds", "sequence": "contig_1", "start": 388147, "stop": 388833, "strand": "+", "frame": 1, "gene": "ybhB", "product": "YbhB/YbcL family Raf kinase inhibitor-like protein", "db_xrefs": [ "COG:COG1881", "COG:R", "SO:0001217", "UniRef:UniRef50_B5ZBF0", "UniRef:UniRef90_A0AAC9T0J3" ], "nt": "GTGCATCATAAGCTATCAACAAACCGAAACAATATTTTATATTTAGACGATTTTGAAGCGAAATTAAATGAAGCAAAAATTATTGCCACAGGTTCTACTTATGTTTGTGCTCCACAGATTAGTTTTGAAGATGATAAATTAATTATTGGTTCATTAGAGCATAAACATCCTTATTTGTTTGCTAATTGTGAAAATGAATATCATGTAATAGAAGATGTAATTGTTACAGATATTAATAAGAATATTTATAGTGAAAATCAACTAGATAATTTTAAAGATCTAGAACAAGCAAAAATTAAAATTATTGATTCTAGTGATGCTTTATCGTATGCAGTTATAATTACTTCAAATCATCTATTTAAAAAATTTGGTCGTCCAGTTGTTAATTATGCACACGTTATCGAGAAAAAAACTAATAAACGAATTAAGTTCAATAATTCCTACAAATTAAATCATGCCTTTAGCGAAATTAGTCCTTCAAAATATGATCATTTAGGTAATGAATTATTGATTTTAGAAGATAAAGATGCTAATTTTGAAAGTGGACTATTCACAATTCATGTGTTTGGATTAAATAAAAAAATTAAGTATTTAAATAAGATTGATAGTGTTGCAAATTCAATTAGTGATTTATTTGAAATGATTAGCGGTAGTGTTATTTCTTATAAAGCGAAGTTATTTAAGTAA", "aa": "MHHKLSTNRNNILYLDDFEAKLNEAKIIATGSTYVCAPQISFEDDKLIIGSLEHKHPYLFANCENEYHVIEDVIVTDINKNIYSENQLDNFKDLEQAKIKIIDSSDALSYAVIITSNHLFKKFGRPVVNYAHVIEKKTNKRIKFNNSYKLNHAFSEISPSKYDHLGNELLILEDKDANFESGLFTIHVFGLNKKIKYLNKIDSVANSISDLFEMISGSVISYKAKLFK", "aa_hexdigest": "5a29b481eced82c425a4d4690d734c04", "start_type": "GTG", "rbs_motif": null, "genes": [ "ybhB" ], "truncated": "5-prime", "pseudogene": { "start": 387847, "stop": 388833, "observations": { "mutation": [ 387859, 387940 ], "stop": [ 387859, 387940 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0J3", "query_cov": 1.0, "subject_cov": 0.5876288659793815, "identity": 0.996, "score": 448.0, "evalue": 7.95e-157, "gene_start": 1, "gene_end": 228, "reference_start": 161, "reference_end": 388, "reference_sequence": "MYKLIFNLCIYIKNKGKFEYMVENKFLKQIKEGEQIKIEIKGLDKFNNFDEQNYGLNNISPQIRWSPVLETASYAVAIIDYEAVGGAPFVHWYALNIFEPELALNVANLGYANLYQGENSTSKHFSHSILDANYQTDIANNYFPPCPPNKSHKYEIKVYAVHHKLSTNRNNILYLDDFEAKLNEAKIIATGSTYVCAPQISFEDDKLIIGSLEHKHPYLFANCENEYHVIEDVIVTDINKNIYSENQLDNFKDLEQAKIKIIDSSDALSYAVIITSNHLFKKFGRPVVNYAHVIEKKTNKRIKFNNSYKLNHAFSEISPSKYDHLGNELLILEDKDANFESGLFTIHVFGLNKKIKYLNKIDSVANSISDLFEMISGSVISYKAKLFK" }, "paralog": false, "identity": 0.9939024390243902, "score": 654.0, "evalue": 3.04e-240, "description": "Internal stop codon at 387859, 387940. Nonsense mutation around 387859, 387940.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0J3", "gene": "YbhB", "product": "YbhB/YbcL family Raf kinase inhibitor-like protein", "uniref50_id": "UniRef50_B5ZBF0", "cog_id": "COG1881", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0J3", "UniRef:UniRef50_B5ZBF0", "COG:COG1881", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBF0" ], "product": "YbhB/YbcL family Raf kinase inhibitor-like protein" } }, "id": "GPKLONFDEH_680", "locus": "GCA000006625_00657" }, { "type": "cds", "sequence": "contig_1", "start": 388870, "stop": 389001, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGATCGTAAACAAATTCATGTTAAAACTAGATTAACAATTAATGAGTTAAATAAGTTTTATACTTTAATTAGAGTCCAAAAACAAAGCTTAAAAGAGATTGAAGATAAAATCAAAAAACTTGATTGA", "aa": "MIDRKQIHVKTRLTINELNKFYTLIRVQKQSLKEIEDKIKKLD", "aa_hexdigest": "aab7a97437fe4eb51db0e7868d81fb01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5269.2147, "isoelectric_point": 9.89874973297119 }, "id": "GPKLONFDEH_681", "locus": "GCA000006625_00658" }, { "type": "cds", "sequence": "contig_1", "start": 389065, "stop": 389445, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AD06", "UniRef:UniRef90_A0AAC9T3L7" ], "nt": "ATGACACCATATGTTATTTCACAAGTTCAAGATCTGGTTAGTGTTTATGATGTAAGACAAATTAGTGAAATAATTCTTGAATTAAATCAAGTTAATATTCAAATTCAAACTAAAGATAGTGAGTTTTATATTAAAAATAATAAAAAATTAAGTTTAATTAGTAGTGAATTTGAAGATTATCAATTAAAAAATTATGATGCATATGAACATTTTAAAATTACAATGGATGAACCTAAAATTCATACTTTAAATCATCAAATGCCATTTGATTCAAAATTAAACATCAAAACCATCATTTTTATAGTTTTAACTTCTTGTTTATTATGTTTATTAATCGTCATAATTATATGTGCATTATTAATTGCAGGAGTTATTAATTAA", "aa": "MTPYVISQVQDLVSVYDVRQISEIILELNQVNIQIQTKDSEFYIKNNKKLSLISSEFEDYQLKNYDAYEHFKITMDEPKIHTLNHQMPFDSKLNIKTIIFIVLTSCLLCLLIVIIICALLIAGVIN", "aa_hexdigest": "b959a57a1b0809b8595573e7342cdfd3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 388870, "stop": 389445, "observations": { "mutation": [ 388999 ], "stop": [ 388999 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3L7", "query_cov": 1.0, "subject_cov": 0.6596858638743456, "identity": 1.0, "score": 229.0, "evalue": 5.85e-75, "gene_start": 1, "gene_end": 126, "reference_start": 66, "reference_end": 191, "reference_sequence": "MIDRKQIHVKTRLTINELNKFYTLIRVQKQSLKEIEDKIKKLDWINFSEIKNQNLFDEFKFDYKNMTPYVISQVQDLVSVYDVRQISEIILELNQVNIQIQTKDSEFYIKNNKKLSLISSEFEDYQLKNYDAYEHFKITMDEPKIHTLNHQMPFDSKLNIKTIIFIVLTSCLLCLLIVIIICALLIAGVIN" }, "paralog": false, "identity": 0.9947643979057592, "score": 372.0, "evalue": 8.43e-134, "description": "Internal stop codon at 388999. Nonsense mutation around 388999.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3L7", "uniref50_id": "UniRef50_A0AAP9AD06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3L7", "UniRef:UniRef50_A0AAP9AD06" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AD06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AD06" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14609.003399999978, "isoelectric_point": 5.224890327453611 }, "id": "GPKLONFDEH_682", "locus": "GCA000006625_00659" }, { "type": "cds", "sequence": "contig_1", "start": 389445, "stop": 390119, "strand": "+", "frame": 3, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "COG:COG0283", "COG:F", "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0005737", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431", "RefSeq:WP_006688521.1", "SO:0001217", "UniParc:UPI000012DD0E", "UniRef:UniRef100_Q9PQE9", "UniRef:UniRef50_Q9PQE9", "UniRef:UniRef90_Q9PQE9" ], "nt": "ATGAAAAAATATATCAGTATCGCTATTGATGGACCTTCAGGTTCTGGAAAAAGTACAGCAGCAAAAGAATTAGCCAAGAAATTGGGATTTTTATATATTAACACTGGTTTAATGTATCGTGCTTATGCTTATTTTTTATCTATTAATAAATTAGATATTAATGTTAACGAAATGGATTGTATTAATGCCATTAAAAATGCTAATTTTATTTTTAATGGTGATAATGTGAAAATTAATGACAACGATGTTAGTCTTATCTTAAGAAGTAATGATGTGGCAATGATTGCTAGTGTTGTATCTGCTAATGAAAAAATTCGAGATTTAGCAACTAATGAACAACGCAGAATTGCTAGTGAAAACAACGTTGTTATGGATGGGCGCGATATTGGAAGTATTGTTTTAACTAATGCTGATCTTAAATTTTATTTAAATACGAGCATTCAAACACGCGCTAAACGACGTTTGTTTCAGAATAAAGATATTGAAAATTTGGATTATGAATCAATATATAACGATATTAAAGAACGTGATCATCGTGATATGACTCGCAAAATTGCTCCACTTAAAAAAGCAAACGATGCTGTTGAAATTTTTAACGATAACATGAAATTAGATGAATGTGTACAACATTTGTATGAAATTTATTTAAAAAAAATAAAAAAGCTAAATTTTTAA", "aa": "MKKYISIAIDGPSGSGKSTAAKELAKKLGFLYINTGLMYRAYAYFLSINKLDINVNEMDCINAIKNANFIFNGDNVKINDNDVSLILRSNDVAMIASVVSANEKIRDLATNEQRRIASENNVVMDGRDIGSIVLTNADLKFYLNTSIQTRAKRRLFQNKDIENLDYESIYNDIKERDHRDMTRKIAPLKKANDAVEIFNDNMKLDECVQHLYEIYLKKIKKLNF", "aa_hexdigest": "82b475fa51afafc767ee13b88fc73df0", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000012DD0E", "ncbi_nrp_id": "WP_006688521.1", "uniref100_id": "UniRef100_Q9PQE9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DD0E", "RefSeq:WP_006688521.1", "UniRef:UniRef100_Q9PQE9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQE9", "uniref90_id": "UniRef90_Q9PQE9" }, "psc": { "uniref90_id": "UniRef90_Q9PQE9", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_Q9PQE9", "cog_id": "COG0283", "cog_category": "F", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0005737", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQE9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE9" ], "product": "Cytidylate kinase" }, "genes": [ "cmk" ], "id": "GPKLONFDEH_683", "locus": "GCA000006625_00660" }, { "type": "cds", "sequence": "contig_1", "start": 390196, "stop": 390483, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688435.1", "SO:0001217", "UniParc:UPI00000C1C29", "UniRef:UniRef100_A0AAC9T2B9", "UniRef:UniRef50_A0AAC9T2B9", "UniRef:UniRef90_A0AAC9T2B9" ], "nt": "ATGAATTTTGATATTAATTTAATTAATGAAAATGATTATGTTTTTAGTGGATACATTATTTTAAAAAAACACAATGAAGAAATTATCGAATTACTAACTTATTTAGGCGATGATCCTATTAATGATGAAGCCAAAATCATAACTTTATATAATAATGATCATATTGATCTAAAAAATCTTAATGATGGAGATTTAATTATTGTAAAAGCAAGTTTAATTATAAATAATAAAAATAACAAACAATTATTGCTTAAAGATATTAATAAACTAGAGTTAAGTATTAACTAA", "aa": "MNFDINLINENDYVFSGYIILKKHNEEIIELLTYLGDDPINDEAKIITLYNNDHIDLKNLNDGDLIIVKASLIINNKNNKQLLLKDINKLELSIN", "aa_hexdigest": "87ab8470e28c9fbdc23c837ccd297448", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1C29", "ncbi_nrp_id": "WP_006688435.1", "uniref100_id": "UniRef100_A0AAC9T2B9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C29", "RefSeq:WP_006688435.1", "UniRef:UniRef100_A0AAC9T2B9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T2B9", "uniref90_id": "UniRef90_A0AAC9T2B9" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T2B9", "uniref50_id": "UniRef50_A0AAC9T2B9" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2B9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10983.421999999984, "isoelectric_point": 4.559362602233888 }, "id": "GPKLONFDEH_684", "locus": "GCA000006625_00661" }, { "type": "cds", "sequence": "contig_1", "start": 390604, "stop": 391329, "strand": "+", "frame": 1, "gene": "plsC", "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "SO:0001217", "UniRef:UniRef50_Q9PQE7", "UniRef:UniRef90_Q9PQE7" ], "nt": "ATGATTTTAGCGGCTATGATGGCAAATAAATATAAAAATGATCCAGAGTCTGTTAGCGTTAATGAGCGCTATAAAAAAATAATGGGTATTATTGATGCTATTTTATATTTAAAAAATATTAAAGTAGATTTGATTGGTAAAGAAAATGTTAGTATAAAAAGCATGTTATACATAGGTAATCACAAATCTAATGGTGATCCAATTGTTTTATTAAAGGCTCTTTATCAAATTAGCCCTCATAATGTGCGTTTTGTTGCTAAAATTGAATTAAAAAAATCATTTTTAAAATTTTTATTTAATTTAATTGATGTAATTTACATTGACCGTAATAATTTAAGACAATCAATTTCAACAATTAATGAACAAGTTAATGCTTTAAAACAAGATTATCGTGGATTAGTTATTTTTCCAGAAGGAACACGAAATTTTAATCATGAATTTAATGAGTTTAAATCAGGCGCTTTAACAGCTGCTTATAATACATTATCTTCGATTCAACCTTTTGTGATTTTTAACTCACAAGGTTCGTTTGAAATGAAAAACGATCAAAAGCAAAAGTTAAGATCAAATCCTTTTAAAACCCAACATATTAAAGTTAGTTTTTTAGAACCAATTCATTCTTTTAATTACATTAATATTAATAAAGATATTTTAATGCGTAAGATTCAATCAAACATTAAAAAAAATTATGATGAATTGTTAAAATCAACAATTAAAACAAATTAA", "aa": "MILAAMMANKYKNDPESVSVNERYKKIMGIIDAILYLKNIKVDLIGKENVSIKSMLYIGNHKSNGDPIVLLKALYQISPHNVRFVAKIELKKSFLKFLFNLIDVIYIDRNNLRQSISTINEQVNALKQDYRGLVIFPEGTRNFNHEFNEFKSGALTAAYNTLSSIQPFVIFNSQGSFEMKNDQKQKLRSNPFKTQHIKVSFLEPIHSFNYININKDILMRKIQSNIKKNYDELLKSTIKTN", "aa_hexdigest": "9b38e1a2f8bbaa0d31cd63a16d679f7b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQE7", "query_cov": 1.0, "subject_cov": 0.9128787878787878, "identity": 0.9129999999999999, "score": 437.0, "evalue": 4.3e-154, "valid": true, "gene": "PlsC", "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "uniref50_id": "UniRef50_Q9PQE7", "cog_id": "COG0204", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_Q9PQE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE7" ], "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase" }, "genes": [ "plsC" ], "id": "GPKLONFDEH_685", "locus": "GCA000006625_00662" }, { "type": "cds", "sequence": "contig_1", "start": 391470, "stop": 392345, "strand": "+", "frame": 3, "gene": "scpA", "product": "Segregation and condensation protein A", "db_xrefs": [ "COG:COG1354", "COG:L", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "KEGG:K05896", "RefSeq:WP_006688570.1", "SO:0001217", "UniParc:UPI000165F8C6", "UniRef:UniRef100_Q9PQE6", "UniRef:UniRef50_Q9PQE6", "UniRef:UniRef90_Q9PQE6" ], "nt": "ATGAGTGAAAAGCAAATCCAAAATAGTTTATTTGAATATAAGTTTATTGATTTTAACGGTCCATTAGATACATTGTGTGTTTTAATTAAACAAAAACGTTTAGATATTAATAATTTAGATATTTTAGAGTTGTCAAAACAATATGTTAATTTTGTAAATCAATTAATTAAAACGATCGATATTGATATTTTAGGTGACCATTTAGCAATGGCTTCTTATTTACTAGAACTTAAGACTCGAATGTTGATGCCAACTGTTGATGAAAAACAAATTATGAGTATTGAAGAAGATCGCCAAAATTTAATTGATCGTTTAATTGAATACAATGGTTATAAAAATTTAAGCGAACATTTAAAACAACGTTTTGAATTTAGAGCAACTATGATGGATTTACCACAACAAGATTATGAGCAATTTTATTTAAAAGATGCAATTTATAAGCCTTTGCCAAATCATTTAGACTCCATGATCTTAAAAAATATTATGGATAAAATCATTTATGAAAATGAATTAAAAAATTATAAAATTAATAAGATTAAAGTTCATGAATATGATGTTAAACAATTAGAACAATTGTTATTAAATTATTTAAAACAATCGCCTAATCAACAAGCAAGTATGCTTAGTTTTTTTGATATACAAGCAGTGATTGATAAAAATAAACGTTTTTTTGCAATAATGTTTTTAATTATTTTAATTTTAATTAATCGTAATGAAATTTTATTTGAAGAATTAGATAATGATTATTTATTAAAAATTAATATTGAAAGAGTTGTTGATGATTATAATTCTAGTAGTGTAGAACAAGCTAAAAATTTAACAAGTAATTTAACAAAAGATACAATTATTAATTTTAAAGGTGAAGACAATGAATAA", "aa": "MSEKQIQNSLFEYKFIDFNGPLDTLCVLIKQKRLDINNLDILELSKQYVNFVNQLIKTIDIDILGDHLAMASYLLELKTRMLMPTVDEKQIMSIEEDRQNLIDRLIEYNGYKNLSEHLKQRFEFRATMMDLPQQDYEQFYLKDAIYKPLPNHLDSMILKNIMDKIIYENELKNYKINKIKVHEYDVKQLEQLLLNYLKQSPNQQASMLSFFDIQAVIDKNKRFFAIMFLIILILINRNEILFEELDNDYLLKINIERVVDDYNSSSVEQAKNLTSNLTKDTIINFKGEDNE", "aa_hexdigest": "0839aff554c5805ce513161be21f1904", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8C6", "ncbi_nrp_id": "WP_006688570.1", "uniref100_id": "UniRef100_Q9PQE6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8C6", "RefSeq:WP_006688570.1", "UniRef:UniRef100_Q9PQE6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQE6", "uniref90_id": "UniRef90_Q9PQE6" }, "psc": { "uniref90_id": "UniRef90_Q9PQE6", "gene": "scpA", "product": "Segregation and condensation protein A", "uniref50_id": "UniRef50_Q9PQE6", "cog_id": "COG1354", "cog_category": "L", "kegg_orthology_id": "K05896", "go_ids": [ "GO:0005737", "GO:0051301", "GO:0007059", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE6" ], "product": "Segregation and condensation protein A" }, "genes": [ "scpA" ], "id": "GPKLONFDEH_686", "locus": "GCA000006625_00663" }, { "type": "cds", "sequence": "contig_1", "start": 392338, "stop": 392460, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATGATAAAAAAAATAAAAAGAATTTATTAAATAATGAATTAGATTTAGCTAAATATACATTACATAATCTATCCGCAGATAACAATGATGATGAAAATGAATTAGAATTAGATTGA", "aa": "MNNDKKNKKNLLNNELDLAKYTLHNLSADNNDDENELELD", "aa_hexdigest": "88782692e0aac2390d0fc069921e3f75", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4673.989499999999, "isoelectric_point": 4.491098976135253 }, "id": "GPKLONFDEH_687", "locus": "GCA000006625_00664" }, { "type": "cds", "sequence": "contig_1", "start": 392698, "stop": 393432, "strand": "+", "frame": 1, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB, long form", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005737", "GO:0007059", "GO:0051301", "GO:0051304", "KEGG:K06024", "SO:0001217", "UniRef:UniRef50_Q9PQE5", "UniRef:UniRef90_Q9PQE5" ], "nt": "TTGCAGAATCAATTAACCAATCATGAATTATTACGTGAAAAATTAAAACCACAAGAAGATTTTAACAAGATATTTAAAACAAAAGATATTAATGTTACTGAAGAAATTAAAATTAAGGATGTTAAAAAAAATAGTAGTGTTTCTTTTCGAAATTCATTTAATATAAATGATTTGAATCAAAATCAAATTACAAACATTAAATCAATTATTGATTCTACACTGTTTTTAGCTGGTGAAGAAGGTGTTTCTTTGCAAGATTTAAAAAGAACAACAGGATTAAATGAGAGTTCTCAACTTAAAACAATTTTAAATCAATTACAAAAAGACTATGACGTTGATGATTCGGGTTTAGTTTTAGTACAATTTGGTGAAAAATTTAAATTATTAACTCAATCCAAAAATAAAGATGCTTTATCAAAATTTGTTACAACAAGTTTTAGAACTCCCTTAAGTCAAAGAAATTTAGAAACGCTTGCTATTATTGCTTATAATCAACCAACAACTCGTGCAAAAATTCAAGCAATTCGTGATCGTGATCCCAAACCTGCCATTGACGCTCTTTTAAAATTGAATTTGATTATAGAAGCAGGCCGTCAAGACACACCAGGTCACCCAATTTTATATACTGTTAGTCAAAAATTTTATGATTTATTTGGTATAAGAAATTTAACAGAATTACCAAGATTAAATAAAGAAATAAATGAATTTAGTCCTATTGATGAATCAACTAACTAA", "aa": "MQNQLTNHELLREKLKPQEDFNKIFKTKDINVTEEIKIKDVKKNSSVSFRNSFNINDLNQNQITNIKSIIDSTLFLAGEEGVSLQDLKRTTGLNESSQLKTILNQLQKDYDVDDSGLVLVQFGEKFKLLTQSKNKDALSKFVTTSFRTPLSQRNLETLAIIAYNQPTTRAKIQAIRDRDPKPAIDALLKLNLIIEAGRQDTPGHPILYTVSQKFYDLFGIRNLTELPRLNKEINEFSPIDESTN", "aa_hexdigest": "60b4dc6c7e6eeb049eb91793265d0bce", "start_type": "TTG", "rbs_motif": null, "genes": [ "scpB" ], "truncated": "5-prime", "pseudogene": { "start": 392398, "stop": 393432, "observations": { "mutation": [ 392458 ], "stop": [ 392458 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQE5", "query_cov": 1.0, "subject_cov": 0.6703296703296703, "identity": 0.996, "score": 462.0, "evalue": 2.18e-162, "gene_start": 1, "gene_end": 244, "reference_start": 121, "reference_end": 364, "reference_sequence": "MNNDKKNKKNLLNNELDLAKYTLHNLSADNNDDENELELDWDGIDDEIIDNNQQIYQKKHINHKTRLFIEKDDKLNNHEQIESGNEIINQPKALSGQDFLEFVNSKLVQKNNNFKQNSKFLQNQLTNHELLREKLKPQEDFNKIFKTKDINVTEEIKIKDVKKNSSVSFRNSFNINDLNQNQITNIKSIIDSTLFLAGEEGVSLQDLKRTTGLNESSQLKTILNQLQKDYDVDDSGLVLVQFGEKFKLLTQSKNKDALSKFVTTSFRTPLSQRNLETLAIIAYNQPTTRAKIQAIRDRDPKPAIDALLKLNLIIEAGRQDTPGHPILYTVSQKFYDLFGIRNLTELPRLNKEINEFSPIDESTN" }, "paralog": false, "identity": 0.997093023255814, "score": 674.0, "evalue": 1.39e-248, "description": "Internal stop codon at 392458. Nonsense mutation around 392458.", "psc": { "uniref90_id": "UniRef90_Q9PQE5", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB, long form", "uniref50_id": "UniRef50_Q9PQE5", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005737", "GO:0007059", "GO:0051301", "GO:0051304" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQE5", "UniRef:UniRef50_Q9PQE5", "COG:COG1386", "COG:K", "KEGG:K06024", "GO:0005737", "GO:0007059", "GO:0051301", "GO:0051304" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE5" ], "product": "Segregation and condensation protein B" } }, "id": "GPKLONFDEH_688", "locus": "GCA000006625_00665" }, { "type": "cds", "sequence": "contig_1", "start": 393571, "stop": 393822, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAACAGTTTCTGAATCATCATTTATAAATGATTCATCTTGTTCTAAAAAAGTTAAAACTTGCGGTATTAAATCATTTGTTGTTGTTTCAAAAACTTCAATTTCATTAACGTGTTTTTTAGCTTCTTCAACCGGAACTTTTCCTGGACGAAAACCAGGAATTTTTAATTTTGAAGCTGTTTTTTTTGCAATTTTTTCAAATGATTCTTTGTGTTTTTTCTCTCATTCTGATTTTTCAATTAAAATTTGA", "aa": "MSTVSESSFINDSSCSKKVKTCGIKSFVVVSKTSISLTCFLASSTGTFPGRKPGIFNFEAVFFAIFSNDSLCFFSHSDFSIKI", "aa_hexdigest": "49b981f263575ab226877fdde041b336", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9000.243699999992, "isoelectric_point": 8.817034721374515 }, "id": "GPKLONFDEH_689", "locus": "GCA000006625_00666" }, { "type": "cds", "sequence": "contig_1", "start": 393938, "stop": 394423, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00254.34" ], "nt": "GTGAATGAAGCTTTTGAACATGAAGTTAATGCTGAAATTGAACATGCATTAAATTCTAAATCACAAAGACGTGTTAAAGATGATAATGAATTCATTGAAAAAGGGGATGAAGTTCGATTTGATTTTAAAGGGATGATTGATAGTGTGCCTTTTGAAGGTGGAAGTGCTAAAGATCATTTATTAACAATTGGATCTAATCAATTTATTCCAGGATTTGAAGATCAAATGATAGGTTTAAAAAAAGATGAACGTAAAAATATTAATGTTAAATTTCCAGATGATTATCATGCAACCGATTTAGCAGGCAAAGCAGCAGTATTTGAAGTTTTTATCAAAGAAATTACAAATGTAAAGCCACAAGAATTAAATGATGAATTTGCTAAATCATTTAATTTGCCTAATGTTAATACAGTTCAAGAATTAAAAGATTATATTCACAATCAAATTGTTTTAGCTAAACAAGAAAAGAATAGTGAACGTGCCTGA", "aa": "MNEAFEHEVNAEIEHALNSKSQRRVKDDNEFIEKGDEVRFDFKGMIDSVPFEGGSAKDHLLTIGSNQFIPGFEDQMIGLKKDERKNINVKFPDDYHATDLAGKAAVFEVFIKEITNVKPQELNDEFAKSFNLPNVNTVQELKDYIHNQIVLAKQEKNSERA", "aa_hexdigest": "ec5e02afec53aba7ee3c2dff2b354668", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00254.34", "name": "FKBP_C", "length": 91, "aa_cov": 0.5093167701863354, "hmm_cov": 1.0, "evalue": 1.779012795206182e-18, "score": 62.31227493286133, "start": 30, "stop": 111 } ], "seq_stats": { "molecular_weight": 18427.339099999994, "isoelectric_point": 4.983608818054199 }, "id": "GPKLONFDEH_690", "locus": "GCA000006625_00667" }, { "type": "cds", "sequence": "contig_1", "start": 394562, "stop": 394876, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTAGTAAAAAATCAGAAGCACAATTTCAAGAAGACTTGATAAGACAAGCTAAAGAAACTATAACTTTAGCACTGTTAGTTGATGATATTGCTGAAACACAAAATATTGTTGTATCTGATGAAGAAGTTAAAGAACGTGTTGCGGAAATGGCTAAACTATACCAAGGGGAAGAACAAGCTGTTATTGAACAATTATCAAAAAATCCTGATGCTGTTAAAGAGTTCTTGTTACATAAAAAAGTTGTTAATTATTTAATTGACTTAAACAAAAATAATCAGCCAAAAAATACAACTTTATCTTCAAAATAA", "aa": "MEFSKKSEAQFQEDLIRQAKETITLALLVDDIAETQNIVVSDEEVKERVAEMAKLYQGEEQAVIEQLSKNPDAVKEFLLHKKVVNYLIDLNKNNQPKNTTLSSK", "aa_hexdigest": "bcde3a53e28c2ae25ef66976e6ac2c7b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11892.357999999986, "isoelectric_point": 4.914037895202636 }, "id": "GPKLONFDEH_691", "locus": "GCA000006625_00668" }, { "type": "cds", "sequence": "contig_1", "start": 395023, "stop": 395940, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACCTATCTTAATATCACGAGCAATTGTTGTTTTACCTTATGAAACAACAACAATTGAAGTAGGACGCCCTAAATCAATCCAAGCGATTGATTTAGCTAAACAATCTTCAAGTAAAGAAATTATTATTATTTCACAAAAAGATATTGATACTGATGAAGTTGTTAATTTTGATGAATTGTATAAAGTAGGAACGTTAGTTAAAATTAAATCTATTATTGATAATTTTGATGAAGGTTATTCAATTGAAGTTGAAGGATTAAAAGCTGTTTACATTAATAATGAGAATGATGTTATTGATGCTATTGAATATGAATATGAAGATGTCATTACAAACCCTATTTTATCAAATAAAGATGAAATAGCAATCAATGGTATTAATTCTGAAATTTTCAATATAATTAACAAGCGTTCAAGACATAAAAATATCAATTTTGATAATATGCACGCTTTAATTTCACTTGAAAAAGAAAAGTTTGCTTATTTAGCTGCGGCAACTTATATTAATGATTATGATAATGAAATTAGTGAAAAAACTATTGAAGATCGAATTAATATCTTATTGCAACCTAATTTATTATTAGTTCATGAGACAATTTTACATTTATTATTTGATCAATTAGTAGATAAGCGCGTTATTGAAGATGAAGTTGAAAAAACTATTACTGATAAAATTAATAATAATTTTCAAAAACAACAACGTGAGTTCTATTTACGCGAAAAATTAAAAGTTGTTAAAGAACAATTAGGTGAACTAAGTAGTCGTGAAGAAGACGCTGATAAAATTCGTGCAAAAATTGAAGATTTAGAATTACCACCAAATGTTAAAGAACGTGCTTTAGCTGAATTAAATCGTTTTGAGAATGCTATGTCATCAAATGAATCATCAGTTATTAAATCATATCTTGATTGA", "aa": "MKKPILISRAIVVLPYETTTIEVGRPKSIQAIDLAKQSSSKEIIIISQKDIDTDEVVNFDELYKVGTLVKIKSIIDNFDEGYSIEVEGLKAVYINNENDVIDAIEYEYEDVITNPILSNKDEIAINGINSEIFNIINKRSRHKNINFDNMHALISLEKEKFAYLAAATYINDYDNEISEKTIEDRINILLQPNLLLVHETILHLLFDQLVDKRVIEDEVEKTITDKINNNFQKQQREFYLREKLKVVKEQLGELSSREEDADKIRAKIEDLELPPNVKERALAELNRFENAMSSNESSVIKSYLD", "aa_hexdigest": "a9fd54c3e14425bf78ad42a309ab7912", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35177.49370000007, "isoelectric_point": 4.775748634338378 }, "id": "GPKLONFDEH_692", "locus": "GCA000006625_00669" }, { "type": "cds", "sequence": "contig_1", "start": 395989, "stop": 397398, "strand": "+", "frame": 1, "gene": "lon", "product": "endopeptidase La", "db_xrefs": [ "COG:COG0466", "COG:O", "EC:3.4.21.53", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565", "SO:0001217", "UniRef:UniRef50_B1AIY7", "UniRef:UniRef90_B1AIY7" ], "nt": "ATGTCTGTAAGAACTCATTTAGATGATAATCACTATGGTATTGAAAAGGTAAAAGAAAGAATTTTAGAGTATTTAGCATTACGTATGCGTAACCCTAATCTTAAAGGTCCAATCATTTGTTTAGTTGGCCCTCCAGGTGTTGGTAAAACATCACTTGTTACTTCGATTGCACAAGCTTTAAATAAAAAATTTGTTAAAGTTAGTTTAGGTGGTGTGCGAGATGAATCTGAAATTCGAGGTCATCGTAAAACTTATGTTGGAGCTATGCCCGGACGTATTATTAAGGGTATGAAAAAGGCTGGCGTTATCAATCCTTTATTCCTATTAGATGAAATTGATAAAATGACATCTGATCAACGAGGAGATCCTGCTGCCGCAATGCTTGAAGTATTAGATCCTGAACAAAATAAAAATTTTTCAGATAATTACATTGAAGAAGAATATGATTTGAGCAAAGTGATGTTTATGGCAACAGCTAATTATTATCAACAAATTCCTTATGCTTTGATTGATCGTTTAGAAGTAATTGAACTATCGAGTTATACAGCAATTGAAAAACGTGAAATAGCAAAATCACATCTATTAAAAAGAATTTTTATGGATGCTAAATTAAATGAAAATGAACTTATTTTTACTGATGATGCTTTAGATTTTATTATTAATCATTATACTAAAGAAGCTGGAGTACGAGAATTAGATCGTCAATTAGGTCATATTGTACGCAAGTACATTGTTGAAACTTATAAGAATAAAAATAATCAATCACCTTTGAATTTAAAAGTTGATGAAGCCATGATTATTAAATATTTAGGTAAAATTAAGTTTGATTTTAATAAAAAAGAAGAGACAACAATTCCAGGAATTGTTAATGGAATGGCTTATACAGCGGCTGGTGGCGATTTATTGCCTATTGAAGTTAATCATTCAACCAATGGAAAAGGTGGAAATGTAACTATTACTGGAAATCTTGAAAAAACAATGAATGAATCTGTATCTGTTGCTTTAGGTTTTGTTAAAGCAAATGCAGATAAATATGGCATTGATACTAAAAAAGTTTCTTTTAAAGAAATTGATATTCATGTTCATGTTCCAAGCGGAGGAATTCCTAAAGATGGACCGTCTGCTGGAATTGCGATTACAACAGCAATTATTAGTTCATTAAGTCAAAGACCAGTGCGTACAACACTATCAATGACAGGAGAAATTATGCTACGTGGTAATGTTGGAATTATTGGTGGTGTTAAAGAAAAAGTTATTTCAGCATATCGTGCTGGAGTTCGTGAAATTATTTTACCAATTGATGACGAACGTTACTTAGAAGATGTTCCAAAGTATATATTAGATGATATTAAAATTCATTTGGTAAAACACTATGATGAAGTTTATAATATTGTTTTTGGAGAAAAATAA", "aa": "MSVRTHLDDNHYGIEKVKERILEYLALRMRNPNLKGPIICLVGPPGVGKTSLVTSIAQALNKKFVKVSLGGVRDESEIRGHRKTYVGAMPGRIIKGMKKAGVINPLFLLDEIDKMTSDQRGDPAAAMLEVLDPEQNKNFSDNYIEEEYDLSKVMFMATANYYQQIPYALIDRLEVIELSSYTAIEKREIAKSHLLKRIFMDAKLNENELIFTDDALDFIINHYTKEAGVRELDRQLGHIVRKYIVETYKNKNNQSPLNLKVDEAMIIKYLGKIKFDFNKKEETTIPGIVNGMAYTAAGGDLLPIEVNHSTNGKGGNVTITGNLEKTMNESVSVALGFVKANADKYGIDTKKVSFKEIDIHVHVPSGGIPKDGPSAGIAITTAIISSLSQRPVRTTLSMTGEIMLRGNVGIIGGVKEKVISAYRAGVREIILPIDDERYLEDVPKYILDDIKIHLVKHYDEVYNIVFGEK", "aa_hexdigest": "802b673f74080daba6e9994be1b061ce", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025901479", "query_cov": 0.9701492537313433, "subject_cov": 0.8956692913385826, "identity": 0.5489999999999999, "score": 489.0, "evalue": 4.25e-167, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A7C6Y0Z4", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "genes": [ "lon" ], "truncated": "5-prime", "pseudogene": { "start": 395689, "stop": 397398, "observations": { "mutation": [ 395938, 395956 ], "stop": [ 395938, 395956 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_B1AIY7", "query_cov": 1.0, "subject_cov": 0.5929203539823009, "identity": 1.0, "score": 918.0, "evalue": 0.0, "gene_start": 1, "gene_end": 469, "reference_start": 323, "reference_end": 791, "reference_sequence": "MKKPILISRAIVVLPYETTTIEVGRPKSIQAIDLAKQSSSKEIIIISQKDIDTDEVVNFDELYKVGTLVKIKSIIDNFDEGYSIEVEGLKAVYINNENDVIDAIEYEYEDVITNPILSNKDEIAINGINSEIFNIINKRSRHKNINFDNMHALISLEKEKFAYLAAATYINDYDNEISEKTIEDRINILLQPNLLLVHETILHLLFDQLVDKRVIEDEVEKTITDKINNNFQKQQREFYLREKLKVVKEQLGELSSREEDADKIRAKIEDLELPPNVKERALAELNRFENAMSSNESSVIKSYLDWLLDLPWTQQGVDNTDLMSVRTHLDDNHYGIEKVKERILEYLALRMRNPNLKGPIICLVGPPGVGKTSLVTSIAQALNKKFVKVSLGGVRDESEIRGHRKTYVGAMPGRIIKGMKKAGVINPLFLLDEIDKMTSDQRGDPAAAMLEVLDPEQNKNFSDNYIEEEYDLSKVMFMATANYYQQIPYALIDRLEVIELSSYTAIEKREIAKSHLLKRIFMDAKLNENELIFTDDALDFIINHYTKEAGVRELDRQLGHIVRKYIVETYKNKNNQSPLNLKVDEAMIIKYLGKIKFDFNKKEETTIPGIVNGMAYTAAGGDLLPIEVNHSTNGKGGNVTITGNLEKTMNESVSVALGFVKANADKYGIDTKKVSFKEIDIHVHVPSGGIPKDGPSAGIAITTAIISSLSQRPVRTTLSMTGEIMLRGNVGIIGGVKEKVISAYRAGVREIILPIDDERYLEDVPKYILDDIKIHLVKHYDEVYNIVFGEK" }, "paralog": false, "identity": 0.9964850615114236, "score": 1108.0, "evalue": 0.0, "description": "Internal stop codon at 395938, 395956. Nonsense mutation around 395938, 395956.", "psc": { "uniref90_id": "UniRef90_B1AIY7", "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_B1AIY7", "cog_id": "COG0466", "cog_category": "O", "go_ids": [ "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_B1AIY7", "EC:3.4.21.53", "UniRef:UniRef50_B1AIY7", "COG:COG0466", "COG:O", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ] }, "pscc": { "uniref50_id": "UniRef50_B1AIY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1AIY7" ], "product": "Lon protease" } }, "id": "GPKLONFDEH_693", "locus": "GCA000006625_00670" }, { "type": "cds", "sequence": "contig_1", "start": 397484, "stop": 398236, "strand": "+", "frame": 2, "gene": "rpoD", "product": "RNA polymerase sigma factor", "db_xrefs": [ "COG:COG0568", "COG:K", "SO:0001217", "UniRef:UniRef50_Q9PQE2", "UniRef:UniRef90_Q9PQE2" ], "nt": "ATGTCAACAAAACAAAATGAACCATTATTTGTAAATCTAGAGGATTTAAAACAAAAAGTTAAATTATCATTTACTGAAGAATTCTCATTTTCGTATGCACTTTCTGAACTTGAAGCAGGAATTTTTGAAAGTCGTAATTTAAAAGGTGCAGATGCTGGTGATCCTGAAGAAATTTTATTAAATGTGGTTTTAGATGTTAGTAAGAAAAAACGTTCTCGAAATGAAATTAAATTTAATAAACTTCAAAATTATTTTATTCATATGAATTTACGTGAAGAACACTTTAGTGAAATTATTGATGTTCTAGAAGCTATTGGAATTCGTGTGCCTGATTATGATTTGGTGATGCAATCAAAATCAAAGACAAACACCAAAAAAAAGGATGAATATGGTATTGATGATACTTTAGAAATTAGCACATCAAAAATTGGTTTTTCATCAACAACAACCGAAAAAGTTGATGATGGTATTAAAGCTTATTTAGGAGTACTTGGAGAATCAAAAATGTTACGCTCTGATGAGGAAACTGAATATGCTAAAATGGTTATTAGTGACGATCCATCTCTAGTTAAAATTGGTAAAAATCAACTTTATACATCAAATATGCGTTTAGTTACTTCTATTGCTAAAAAATATTTAAATCGTGGATTAGACTTAGAAGATTTAATTCAAGAAGGGTCATCCGGTTTATTAAAAGCAATTGATAAATTTGATCATGAAAAAGGACATAAATTTAGTACATATGCAACATGA", "aa": "MSTKQNEPLFVNLEDLKQKVKLSFTEEFSFSYALSELEAGIFESRNLKGADAGDPEEILLNVVLDVSKKKRSRNEIKFNKLQNYFIHMNLREEHFSEIIDVLEAIGIRVPDYDLVMQSKSKTNTKKKDEYGIDDTLEISTSKIGFSSTTTEKVDDGIKAYLGVLGESKMLRSDEETEYAKMVISDDPSLVKIGKNQLYTSNMRLVTSIAKKYLNRGLDLEDLIQEGSSGLLKAIDKFDHEKGHKFSTYAT", "aa_hexdigest": "42b842bed76bb46208c05447051f0b43", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rpoD" ], "truncated": "3-prime", "pseudogene": { "start": 397484, "stop": 398539, "observations": { "mutation": [ 398234, 398237 ], "stop": [ 398234, 398237 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQE2", "query_cov": 1.0, "subject_cov": 0.5580357142857143, "identity": 0.94, "score": 456.0, "evalue": 1.39e-158, "gene_start": 1, "gene_end": 250, "reference_start": 1, "reference_end": 250, "reference_sequence": "MSTKQNEPLFENLEDLKQKVKLSFTEEFSFSYALSEREAGIFESRNLKGADASNPEEILLNVVLDVSKRKRSRNEIKFNKLQNYFIHMNLRDEHFSEIVDVLENIGIRVPDYELVMQSKSKSTAKKKDEYGIDDTLEISTSKIGFSSTTTEKVDDGIKAYLGVLGESKMLRSDEETEYAKMVISNDPALIKIGKNQLYTSNMRLVTSIAKKYLNRGLDLEDLIQEGSSGLLKAIDKFDHEKGHKFSTYATWWIRQSITRAIADQARQIRIPVHMVETINKLTKAERSLIQELGRDPTAEEIAQAMNKASQAKNQKEQLITAQKVVEIKKLNVDPVSLDKQIGHDEESQFSDFISDDEIISPEKYTEKKALNDQINEMFEKVLNDNEQRVIKMRYGLLPFERPYTLEEVGEHLGVTRERARQIESKAIRKLKHPSKTAKLRSFIGESEN" }, "paralog": false, "identity": 0.9458689458689459, "score": 642.0, "evalue": 3.41e-234, "description": "Internal stop codon at 398234, 398237. Nonsense mutation around 398234, 398237.", "psc": { "uniref90_id": "UniRef90_Q9PQE2", "gene": "RpoD", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_Q9PQE2", "cog_id": "COG0568", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQE2", "UniRef:UniRef50_Q9PQE2", "COG:COG0568", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQE2" ], "product": "RNA polymerase sigma factor SigA" } }, "id": "GPKLONFDEH_694", "locus": "GCA000006625_00671" }, { "type": "cds", "sequence": "contig_1", "start": 398303, "stop": 398830, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04545.22" ], "nt": "ATGGTTGAAACAATTAACAAATTAACTAAAGCCGAGCGAAATTTAATTCAAGAATTAGGAAGGGATCCAACGGCTGAAGAAATTGCGCAAGCTATGAACAAAGTTTCACAAGCAAAAAATCAAAAAGAACAGTTAATCACTGCACAAAAAGTTGTTGAGATTAAAAAATTAAATGTTGACCCAGTTTCATTAGATAAACAAATTGGTCATGATGAAGAATCACAATTTTCAGATTTCATTTCTGATGATGAAATTATTTCTCCAGAAAAATATACTGAAAAAAAAGCATTAAATGATCAGATTAATGAAATGTTTGAAAAAGTTTTAAACGATAATGAACAACGTGTTATTAAAATGCGGTATGGTTTATTACCCTTCGAACGTCCTTATACACTTGAAGAAGTTGGTGAATATTTAGGAGTTACACGCGAACGTGCACGTCAAATTGAATCAAAAGCAATTCGTAAATTAAAACATCCATCAAAAACCGCAAAACTACGTTCATTTATTGGTGAATCCGAAAACTAG", "aa": "MVETINKLTKAERNLIQELGRDPTAEEIAQAMNKVSQAKNQKEQLITAQKVVEIKKLNVDPVSLDKQIGHDEESQFSDFISDDEIISPEKYTEKKALNDQINEMFEKVLNDNEQRVIKMRYGLLPFERPYTLEEVGEYLGVTRERARQIESKAIRKLKHPSKTAKLRSFIGESEN", "aa_hexdigest": "cccd2081e377d6d921486d29701a108d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04545.22", "name": "Sigma70_r4", "length": 52, "aa_cov": 0.29714285714285715, "hmm_cov": 0.9038461538461539, "evalue": 3.841398185076076e-21, "score": 69.9921875, "start": 108, "stop": 159 } ], "seq_stats": { "molecular_weight": 20233.731200000017, "isoelectric_point": 5.631061744689941 }, "id": "GPKLONFDEH_695", "locus": "GCA000006625_00672" }, { "type": "cds", "sequence": "contig_1", "start": 398814, "stop": 399479, "strand": "+", "frame": 3, "gene": "trmK", "product": "SAM-dependent methyltransferase", "db_xrefs": [ "COG:COG2384", "COG:J", "EC:2.1.1.217", "KEGG:K06967", "RefSeq:WP_006688536.1", "SO:0001217", "UniParc:UPI00000C1C2F", "UniRef:UniRef100_A0A2C9DZ44", "UniRef:UniRef50_A0A2C9DZ44", "UniRef:UniRef90_A0A2C9DZ44" ], "nt": "GTGAATCCGAAAACTAGAATTGCTTTTATTGCTGATTTAATTAATGACGCTCAAAATGTTGTCGATGTTGGTAGTGATCACGCATATTTAGCTAAAATCTTATTGGTTAACAATAAAGCACAACATGTTACTAATATAGAAGTTAATCAAGGTCCATTAGAAAACGGAATCAATAATTTGACTAAAAATAATCTTTTAAAACGAACTACTAATATTTTGAATGATGGTTTCAAGGGATTAATTTTGGAACAAACATTTGATTATTGTGTTATTGCAGGGATGGGAGCAACAAGTATAATAGAAATTTTAGAACAGAATAAAAATCAAATTAAAAATTATATTTTACAACCAAATACTCAATCATATAAAATACGTAAATATCTAAATAATCACTCATATCAAATTAAAGTTGAACACATTTTTGTTGAAAAAAAAATTTTTTATGAAATAATCATTTGTTCTAAAACTATTAATGTACCAAAATTAATAGAAAAAGATTATTATATAAGTAGCCAGATGCATAAGGATTCATTGAATTTATATTTTAAATTTTTAAAGAGACGTTATGAATACTTAAAAGCTTTGGATATTTCGCTAGTTAGTGAAAATATAGTTAAAGAATATGAATATATAAAGGAATTTATTAATGAAAAAAATGGATATTAA", "aa": "MNPKTRIAFIADLINDAQNVVDVGSDHAYLAKILLVNNKAQHVTNIEVNQGPLENGINNLTKNNLLKRTTNILNDGFKGLILEQTFDYCVIAGMGATSIIEILEQNKNQIKNYILQPNTQSYKIRKYLNNHSYQIKVEHIFVEKKIFYEIIICSKTINVPKLIEKDYYISSQMHKDSLNLYFKFLKRRYEYLKALDISLVSENIVKEYEYIKEFINEKNGY", "aa_hexdigest": "921fe2f18e6d404483d559f1d2274f36", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C2F", "ncbi_nrp_id": "WP_006688536.1", "uniref100_id": "UniRef100_A0A2C9DZ44", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C2F", "RefSeq:WP_006688536.1", "UniRef:UniRef100_A0A2C9DZ44" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DZ44", "uniref90_id": "UniRef90_A0A2C9DZ44" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DZ44", "gene": "TrmK", "product": "SAM-dependent methyltransferase", "ec_ids": [ "2.1.1.217" ], "uniref50_id": "UniRef50_A0A2C9DZ44", "cog_id": "COG2384", "cog_category": "J", "kegg_orthology_id": "K06967" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DZ44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DZ44" ], "product": "SAM-dependent methyltransferase" }, "genes": [ "trmK" ], "id": "GPKLONFDEH_696", "locus": "GCA000006625_00673" }, { "type": "cds", "sequence": "contig_1", "start": 399811, "stop": 400092, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAACGTTTAGGCTTATTGAATCTTAAGCAAGATGAACAGAATCCATATCTTGTTGTAGGAGAATTAAAATTAGGTGTTAGTGTTGATTACATTAGTCGGATTATTAAACAAAAATTTTTATCACCTATTATTAAATATAACAACGTTTTTAGACTAGAAACAAATCTTAAAAAAATTGGTATTATAGGAGGTAGTGGTTATAAATTTGCTGATGACGCATTTAATCGTTATCAACTAGATATGTTAATTACAAGTGATTTAAAATATCATAATTGA", "aa": "MAKRLGLLNLKQDEQNPYLVVGELKLGVSVDYISRIIKQKFLSPIIKYNNVFRLETNLKKIGIIGGSGYKFADDAFNRYQLDMLITSDLKYHN", "aa_hexdigest": "a4c99b7f7ba938b5def71fa329dff4c8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10710.387899999987, "isoelectric_point": 9.521416282653806 }, "id": "GPKLONFDEH_697", "locus": "GCA000006625_00674" }, { "type": "cds", "sequence": "contig_1", "start": 400129, "stop": 400248, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCATTTGTCTGAATCAATTTTTATAGATGTAATTTATGATGAATTAACAAAATTTTATGGAAATGATGCAAATTTAAATAAATCTTTATCAATTATAAAAATTAACTATATTTAA", "aa": "MNHLSESIFIDVIYDELTKFYGNDANLNKSLSIIKINYI", "aa_hexdigest": "021b7b22d06eb76dfdab67e4e300a2b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4550.1448, "isoelectric_point": 4.8263353347778315 }, "id": "GPKLONFDEH_698", "locus": "GCA000006625_00675" }, { "type": "cds", "sequence": "contig_1", "start": 400278, "stop": 400607, "strand": "-", "frame": 2, "gene": null, "product": "Sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "RefSeq:WP_006688721.1", "SO:0001217", "UniParc:UPI00000C1C30", "UniRef:UniRef100_A0AAC9T0X2", "UniRef:UniRef50_A0AAC9T0X2", "UniRef:UniRef90_A0AAC9T0X2" ], "nt": "ATGTGTATTAGTAAAATAGATATGGCTAAGGTAAAAAAATTTTTTAAAGAATATTTATTTGCTAAATTTCAATGCAAAAATCGAGAACTATATCGTCAATTAAAAGATTATGATCCAAAAGATGATCAAAAATATTTAAAATGGGAGCATTTTGTTGAATATGTTGAGCAAGTTCTAGAAGCATTAGATAAAACTAGTGCTCAAATTATTAAAGAAATTTATATTCAAAATAAAAGAATTTGTGAATTACCTTATAGTTATTCAACATATTATGCATATCGTAAAAAAGCAATTATTGAATTACTTGCATATCTTGATTTAAAAATTTAA", "aa": "MCISKIDMAKVKKFFKEYLFAKFQCKNRELYRQLKDYDPKDDQKYLKWEHFVEYVEQVLEALDKTSAQIIKEIYIQNKRICELPYSYSTYYAYRKKAIIELLAYLDLKI", "aa_hexdigest": "2efdadbda6483303041e757dd1861977", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1C30", "ncbi_nrp_id": "WP_006688721.1", "uniref100_id": "UniRef100_A0AAC9T0X2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C30", "RefSeq:WP_006688721.1", "UniRef:UniRef100_A0AAC9T0X2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0X2", "uniref90_id": "UniRef90_A0AAC9T0X2" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0X2", "product": "Sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0AAC9T0X2" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0X2" ], "product": "Sigma-70 family RNA polymerase sigma factor" }, "genes": [], "id": "GPKLONFDEH_699", "locus": "GCA000006625_00676" }, { "type": "cds", "sequence": "contig_1", "start": 400834, "stop": 401283, "strand": "-", "frame": 1, "gene": null, "product": "Unique hypothetical membrane lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQD8", "UniRef:UniRef90_Q9PQD8" ], "nt": "ATGATTAAAAAAATTAGACCTAGAACTAATAAAATGAGCTTAGAAGATTATTTGATCGCAAGGATGAATTTAATTAATTCATTCAAATTTATACTAAAACGTAAAGTATTATTTATTGAAGGGATCAAAGAATATAACCATCGTTTTGTTTTTAATATGAATGCTGATTATAATTTAATGAGAATAATGAATTATATTTATTCTGGTTTGGCAGAATTAAACATCATTATGGTTATTGATATGGATAGTGATGAAAAAATTTTTGAATTACAAAAAGTAATTAAACAAGATTTTGATAATTTAGATTTAATCATTATAGAAAAAGAAATAGCAGAGTCACTAAAATCAAAATACCAAAATGGTTGTTTAAATAAAAAAACTAATTTAATTAACATTAATAATAAAGAAATTGATCAAAGTCAACAAACTCAAGATATTAACATTTACTAA", "aa": "MIKKIRPRTNKMSLEDYLIARMNLINSFKFILKRKVLFIEGIKEYNHRFVFNMNADYNLMRIMNYIYSGLAELNIIMVIDMDSDEKIFELQKVIKQDFDNLDLIIIEKEIAESLKSKYQNGCLNKKTNLININNKEIDQSQQTQDINIY", "aa_hexdigest": "33fdc0ccb4f2f782b7db014cca8506d6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 400834, "stop": 401583, "observations": { "mutation": [ 401370, 401391 ], "stop": [ 401370, 401391 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQD8", "query_cov": 1.0, "subject_cov": 0.436950146627566, "identity": 1.0, "score": 284.0, "evalue": 5.11e-94, "gene_start": 1, "gene_end": 149, "reference_start": 193, "reference_end": 341, "reference_sequence": "MKTILIVNKSFRKKRSFDTRFLHDSGTIYSNDRFLKIVRESFDAHIKKKSKILFYFMEWWFQTTNYKLNPEISHAKRSLRSWLIEIFFLFCIVALIVCILAGVARPIIDCVIKSITLSRGQFKGDLIIDGQIIMKNLSPSNINGLTGGSSILNVDQWELIPKVWKAYFSSPSVVVLILLVLFFLFFSCLYIYMIKKIRPRTNKMSLEDYLIARMNLINSFKFILKRKVLFIEGIKEYNHRFVFNMNADYNLMRIMNYIYSGLAELNIIMVIDMDSDEKIFELQKVIKQDFDNLDLIIIEKEIAESLKSKYQNGCLNKKTNLININNKEIDQSQQTQDINIY" }, "paralog": false, "identity": 0.9919678714859438, "score": 466.0, "evalue": 2.32e-168, "description": "Internal stop codon at 401370, 401391. Nonsense mutation around 401370, 401391.", "psc": { "uniref90_id": "UniRef90_Q9PQD8", "product": "Unique hypothetical membrane lipoprotein", "uniref50_id": "UniRef50_Q9PQD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQD8", "UniRef:UniRef50_Q9PQD8" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQD8" ], "product": "Unique hypothetical membrane lipoprotein" } }, "id": "GPKLONFDEH_700", "locus": "GCA000006625_00677" }, { "type": "cds", "sequence": "contig_1", "start": 401683, "stop": 401859, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAACAATTTTAATTGTAAATAAATCATTTCGTAAAAAGCGCAGTTTTGACACTCGTTTTTTGCATGATAGTGGAACGATTTATAGTAATGATCGTTTTTTAAAAATTGTGCGTGAAAGCTTTGATGCTCATATTAAGAAAAAAAGTAAAATTCTATTTTATTTCATGGAATGA", "aa": "MKTILIVNKSFRKKRSFDTRFLHDSGTIYSNDRFLKIVRESFDAHIKKKSKILFYFME", "aa_hexdigest": "aee2a06cb70b68d9d43acd9e35eeac21", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7076.253599999998, "isoelectric_point": 10.229021263122558 }, "id": "GPKLONFDEH_701", "locus": "GCA000006625_00678" }, { "type": "cds", "sequence": "contig_1", "start": 402115, "stop": 402309, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAATTAATGAAGGAACAAAACTATTAACAAAATTAATACTAGATAATAAATCATATATTGCTGAAATTAAATTTGGAACATCAACAAATACTTATGATGCAGCAGGTGAAATTGTTTCATCTACAAACCGAATGGTTACATTAACTGAGGTTACTAAAATAGTTAAAGATTTTTATAAAAATGATTATTGA", "aa": "MGINEGTKLLTKLILDNKSYIAEIKFGTSTNTYDAAGEIVSSTNRMVTLTEVTKIVKDFYKNDY", "aa_hexdigest": "60512212ac0e2850146302164323f9ed", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7169.098900000004, "isoelectric_point": 6.124367332458495 }, "id": "GPKLONFDEH_702", "locus": "GCA000006625_00679" }, { "type": "cds", "sequence": "contig_1", "start": 402430, "stop": 402624, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTCAATTATGAAAAACAAATTTTAAAAATTTCTTTACATGTTTCTAAAGGTTTTTATGTTCGTTCTTTTGCTGTTGATTTAGCTACTAAAATTAATAATCTAGCTCATTTATTGACATTAATAAGGACGCAAAGTGGACCTTTTGAGATTAAAGATGCAATTGAAATTGAACAAGTCTATGATTATTGA", "aa": "MDFNYEKQILKISLHVSKGFYVRSFAVDLATKINNLAHLLTLIRTQSGPFEIKDAIEIEQVYDY", "aa_hexdigest": "0eea71d54a267a4df4ca5b9686e6f0dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7449.492499999998, "isoelectric_point": 6.025240135192871 }, "id": "GPKLONFDEH_703", "locus": "GCA000006625_00680" }, { "type": "cds", "sequence": "contig_1", "start": 402590, "stop": 403465, "strand": "+", "frame": 2, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "db_xrefs": [ "EC:2.7.1.26", "EC:2.7.7.2", "GO:0003919", "GO:0005737", "GO:0006747", "GO:0009398", "KEGG:K11753", "SO:0001217", "UniRef:UniRef50_Q9PQD6", "UniRef:UniRef90_Q9PQD6" ], "nt": "ATGCAATTGAAATTGAACAAGTCTATGATTATTGAAATAACATCAACAAATATTTATAAAATTAAGCAACAATTCGTCATTAATGAATTAATTATTGGTTTTTTTGATGGAATTCATTTAGGTCATATGAATTTATTATCAGATCCTAATAACCAAACTATTTTAACTTTTAAAAATATCCCACGTAAAATAAAAAAATTATATGATTTTAATGAGCGAATTCAACAATTAGAAGATTTAGGATTTAAAAGGATTTTTATTTATGATATTGATCAAAACAATTTAAGTGGGGAAGAATTTATTGATCAAATTTTAAAGCCACTAACTCCAAAAAAAATTATTGTTGGTGCTAATTTTACTTATGGCAATAATTTTTGTAATGCATCTAGTCTAAAACAATATTTTAATGTTGAAATTAAAATTATTACTAATGATGTTAGTACAACTAAAATTAAAGAATTAATTATAAATAAACAGGTTGAAATAGCTAATAAATTATTAATTAAACCATATTATCGTGTGGGAAATGTTGTACGTGGCGATCAAATAGCTCGTAATATTGGTTTTAATACTGCTAATATTTTATGTGATAATAATTTAATTGATATTGCTGAAGGTGTTTACAAAGCACAAGTAATTTTTAATAATAAAAAGTATGATAGTGTAGTTTATTTAGGAATCCCTAAAACAATTAATACTCGCTCTTTTTCAATGATTGAAGCACATATTTTAGACTTTAATCAAAATATTTATGACGAAAGAATTAAGATTGTTTTTTTGAAATATTTAGCTCCTAATTTAAAATTTAATAATATAGATGAATTAATCACTGCAATTAAAAATTATATTAAATTGGTTCTTGATAAAACAAATTAG", "aa": "MQLKLNKSMIIEITSTNIYKIKQQFVINELIIGFFDGIHLGHMNLLSDPNNQTILTFKNIPRKIKKLYDFNERIQQLEDLGFKRIFIYDIDQNNLSGEEFIDQILKPLTPKKIIVGANFTYGNNFCNASSLKQYFNVEIKIITNDVSTTKIKELIINKQVEIANKLLIKPYYRVGNVVRGDQIARNIGFNTANILCDNNLIDIAEGVYKAQVIFNNKKYDSVVYLGIPKTINTRSFSMIEAHILDFNQNIYDERIKIVFLKYLAPNLKFNNIDELITAIKNYIKLVLDKTN", "aa_hexdigest": "42f3b7dd4054e4665ae0ad4d9d6dba47", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q9PQD6", "query_cov": 0.9725085910652921, "subject_cov": 1.0, "identity": 0.975, "score": 545.0, "evalue": 1.37e-195, "valid": true, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_Q9PQD6", "kegg_orthology_id": "K11753", "go_ids": [ "GO:0003919", "GO:0005737", "GO:0006747", "GO:0009398" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQD6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQD6" ], "product": "Riboflavin biosynthesis protein" }, "genes": [ "ribF" ], "id": "GPKLONFDEH_704", "locus": "GCA000006625_00681" }, { "type": "cds", "sequence": "contig_1", "start": 403608, "stop": 403814, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATGAAAGCTAAGAAATTTTTAATTACAGGATTATTAGGTGTAGTTAGTTTAGCAACTATTACCACAGTAGCTACAGCTTGTACAAATGGCAATAATCAAAAGGCTAATACAGTAAATAATAAGTCTGATTTAAAAACAAATCCAAATATTAATTTAACTCAACAAGATGTAGAAAATTTGGAAAAACAAAGACAACAATGA", "aa": "MKMKAKKFLITGLLGVVSLATITTVATACTNGNNQKANTVNNKSDLKTNPNINLTQQDVENLEKQRQQ", "aa_hexdigest": "1e4a30e5ac40e86ac7c58d6aeb731d31", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7445.471400000003, "isoelectric_point": 9.807978248596193 }, "id": "GPKLONFDEH_705", "locus": "GCA000006625_00682" }, { "type": "cds", "sequence": "contig_1", "start": 404220, "stop": 404525, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATCACGAGTTTCTGGTTTGCCATCACACTATCCTGCTGTTCTAACACCAAAATTTTTATTTGATCCTAATTTAAATCCAAATTTAAAAAAATATGCTTCTGCTAATTATGCGTTACCTAATTATTACTCTGACCCACTAGATGGGATTATTTTAACAGGAAAAACTTTAGATCGTATTTATGATGCTAAAAAGTTTTCTAATGTTTATAATGCACATGTAGCTGGCAAAACACATTTTGAGACATTCACAGATTTTGCAAAGGCAATGGTTGATGAAACGCGTCGTGGAATTTCACAATGA", "aa": "MQSRVSGLPSHYPAVLTPKFLFDPNLNPNLKKYASANYALPNYYSDPLDGIILTGKTLDRIYDAKKFSNVYNAHVAGKTHFETFTDFAKAMVDETRRGISQ", "aa_hexdigest": "0f58190332ed439183843845d1fdf313", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11374.75419999999, "isoelectric_point": 9.18766345977783 }, "id": "GPKLONFDEH_706", "locus": "GCA000006625_00683" }, { "type": "cds", "sequence": "contig_1", "start": 404565, "stop": 405152, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATGCCTAACTTTACTCCAACAGCTAAAATGGTAGATGTTAATTTTAAACCAGAAGACTTAGAATTCTTGAGTAATGTTTGCATTAATGAACCTATGTATTGTCCAACAATATATAGTGATCCAAATGATAAATATTTACCTGGATTAGGTGCAAAATTCCCTATTCCAGTAAATCATATTTCACAAGTGCAAAATTATATTGATGATTATGGACAAATTGGTGGAAAAGTATTAAATGGTACTGGTAATCCAGAAGCATCAAAACAAGTTGGCCCAACTTTAGGTGATGCTTTTAAAGATACAAGTGACGTTGTAGTTTTCTGTTATAACGAATACGGTTTATTAAATTACAAACCCGGAACTGAAGAAGGCGAAGCTTTAGTACAACAATTTGAAAAGAATTTAACAAATTATGTTAATGGTTTAACAAAAGAAACAAAAAATCAATTTGTGCCAACAAAAGTTTTAAAAGAAAAACCAGTCGTGGGTAAGAATTTCTTTATTGAACGTAAAGGTAAGTTCTATGATGCATGTTATGGTTTTGCTGGTCAAGATTTAGTAGTTAATACATTAAATAAATGA", "aa": "MVMPNFTPTAKMVDVNFKPEDLEFLSNVCINEPMYCPTIYSDPNDKYLPGLGAKFPIPVNHISQVQNYIDDYGQIGGKVLNGTGNPEASKQVGPTLGDAFKDTSDVVVFCYNEYGLLNYKPGTEEGEALVQQFEKNLTNYVNGLTKETKNQFVPTKVLKEKPVVGKNFFIERKGKFYDACYGFAGQDLVVNTLNK", "aa_hexdigest": "c37b8a0d6b2af6d5e38fe9c983afbbd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21746.46000000001, "isoelectric_point": 5.098878288269042 }, "id": "GPKLONFDEH_707", "locus": "GCA000006625_00684" }, { "type": "cds", "sequence": "contig_1", "start": 405290, "stop": 405520, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATAAAACAAGTAAATATTAATAAAAAAGATCTTTCTATTTATAAAAAACTCATTGAAGCAAAAAAATTTGTTAGTACACAAATTCGTCGTCCTTATATGATAACGATTATTGTATTAACATTTATTATCTTAGCTGTTGGTATGTTTTTTATGAATGTTTTTTATGAAAAATCTACATATTTTGATGCGAATTATAATACTCATGTAACCTATTTTCGAACTTGA", "aa": "MQIKQVNINKKDLSIYKKLIEAKKFVSTQIRRPYMITIIVLTFIILAVGMFFMNVFYEKSTYFDANYNTHVTYFRT", "aa_hexdigest": "2a4217d5cfa7c76ef4aacd9d4584236d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9141.78509999999, "isoelectric_point": 9.872446632385255 }, "id": "GPKLONFDEH_708", "locus": "GCA000006625_00685" }, { "type": "cds", "sequence": "contig_1", "start": 405505, "stop": 405621, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATATCCAGCAACCGGCATAGAAATTGCTACAAATAATTTAACGATAGGTGGATACCAATACAATAAAATACTTTTATCATCAGCTACTACTTTAGCTCAAGTTCGAAAATAG", "aa": "MAYPATGIEIATNNLTIGGYQYNKILLSSATTLAQVRK", "aa_hexdigest": "c44ac7f790f5fa8521824da896494b6e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4086.665900000002, "isoelectric_point": 9.398732948303223 }, "id": "GPKLONFDEH_709", "locus": "GCA000006625_00686" }, { "type": "cds", "sequence": "contig_1", "start": 405971, "stop": 406090, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTAATTTTGATTTGTTTATTTGCTTTTTTAGTATTATCACCAAAATTAAAAATTATGCAACGCGATTTTGCTTTGGCAAAATCTTTAGGAATCAAAGTTAATTTAATTTTTTGA", "aa": "MPLILICLFAFLVLSPKLKIMQRDFALAKSLGIKVNLIF", "aa_hexdigest": "d28722996d0aa30185c04702bbae5206", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4419.577899999999, "isoelectric_point": 10.032650566101072 }, "id": "GPKLONFDEH_710", "locus": "GCA000006625_00687" }, { "type": "cds", "sequence": "contig_1", "start": 406094, "stop": 406360, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAGTTTTAACAGCAATTGTAACAATTGCAACTGTTAATATTACAAGTCCCATGATTCTATTAGGTTTAATTATTCCAAATATAGTTAGGGCAACATTTAATAAACATGAACCATTATTTGTATTTTTTGTATCTATTGTATTTTCATTAGCATTGTTAGAAGTTTCATTATTTTTATCACTAAATTATCGTTTTGGTCCTAACTTTTTAATGGCTATTGTTAGTGCATTTGTGTTAATTTTTATTATGCGAAAACACGGATAA", "aa": "MTVLTAIVTIATVNITSPMILLGLIIPNIVRATFNKHEPLFVFFVSIVFSLALLEVSLFLSLNYRFGPNFLMAIVSAFVLIFIMRKHG", "aa_hexdigest": "78af84c2b2b4e56b0210ef0e94320ad2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9827.89349999999, "isoelectric_point": 10.266477394104005 }, "id": "GPKLONFDEH_711", "locus": "GCA000006625_00688" }, { "type": "cds", "sequence": "contig_1", "start": 406366, "stop": 406503, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATCCATCACGATTTATATTTATAATAAAAAATAATATTTTAAATACCATTAACAATGCATCATTTATTGAAAATGGAAATTTCAAGAAAAAATTTCGGGTTTATGCACGCAAGCAACATTTAGCAGCTTGA", "aa": "MKNPSRFIFIIKNNILNTINNASFIENGNFKKKFRVYARKQHLAA", "aa_hexdigest": "772b7f820bc7300daff0f5508763fe41", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5311.176100000002, "isoelectric_point": 11.202945137023924 }, "id": "GPKLONFDEH_712", "locus": "GCA000006625_00689" }, { "type": "cds", "sequence": "contig_1", "start": 406546, "stop": 406641, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAAAATTAATAACCAAGGTCAATTATTAAATAATCGGACTCCTTTAGATTTGTTTAAAGTAAATTATGAAACAATCTTACAAATTTGA", "aa": "MFIKINNQGQLLNNRTPLDLFKVNYETILQI", "aa_hexdigest": "63b31c385971f9c88c5886832a9acb23", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3692.2881000000007, "isoelectric_point": 8.253774452209473 }, "id": "GPKLONFDEH_713", "locus": "GCA000006625_00690" }, { "type": "cds", "sequence": "contig_1", "start": 406675, "stop": 406869, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCCAACGGATTACTAAAAATCGATTAGTTGATACATCAACTCTTGGGATAGGTAATGTTTGTTTGATTGGGTTATTTTTTTTAGCAATATCAATTAATTTTGAAAATTATGGTTCTCAAAAACTTTTTGAAAATTTATTACCTTTTATTTTTATAATATCAGGAGTATGTGCCGGATTAATTTTGTATTGA", "aa": "MFQRITKNRLVDTSTLGIGNVCLIGLFFLAISINFENYGSQKLFENLLPFIFIISGVCAGLILY", "aa_hexdigest": "e55ef750bd793d1e1cd7e992170cafd4", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7161.449999999996, "isoelectric_point": 7.789859962463378 }, "id": "GPKLONFDEH_714", "locus": "GCA000006625_00691" }, { "type": "cds", "sequence": "contig_1", "start": 406794, "stop": 406970, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTTTAAGCTAATTGATAAAGCAATAGCAATAAAATTAATGAATACACCAGAGACAATTAACTTTGTATGGCTAAAACCGGTTCGTTTTTTTGAAAATCAATACAAAATTAATCCGGCACATACTCCTGATATTATAAAAATAAAAGGTAATAAATTTTCAAAAAGTTTTTGA", "aa": "MSFKLIDKAIAIKLMNTPETINFVWLKPVRFFENQYKINPAHTPDIIKIKGNKFSKSF", "aa_hexdigest": "01e2e81074753b1df8607953180071f6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6813.038900000001, "isoelectric_point": 9.998482322692869 }, "id": "GPKLONFDEH_715", "locus": "GCA000006625_00692" }, { "type": "cds", "sequence": "contig_1", "start": 407092, "stop": 407469, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTTCTGCTAAATTTAAAGTTGTTATTACAGATACAATGATTGCTGAACAATTAGGATTAAAAGTAAAATTATTGAATTTAGAAATGATTTTTATCTCATCTTGTTTAACAGCTGTTTCATATATTATGGGAGGAAATGTATTATTTTTAGGAATTGCTGCAGCAAATTTAGGGTATGCGGTTTTTGGTAATCGCTTTAGTTCATCAATAATTTTTTCAAGCTTCATCACCATTATCTTTTTAATTTTTGGTCAATTTATTTTAGATAATATCGTTCAAAATTTAACTAGTTTGGTATTTGACGCCCCTATTGTAACTCCTTTATTAGTAGCTCCAATATTTACATTTATTATCTTATTTAAAAATAATATTTAA", "aa": "MRSAKFKVVITDTMIAEQLGLKVKLLNLEMIFISSCLTAVSYIMGGNVLFLGIAAANLGYAVFGNRFSSSIIFSSFITIIFLIFGQFILDNIVQNLTSLVFDAPIVTPLLVAPIFTFIILFKNNI", "aa_hexdigest": "2458d155957c8089e6e453be5cc43da2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13747.385399999977, "isoelectric_point": 8.930628013610843 }, "id": "GPKLONFDEH_716", "locus": "GCA000006625_00693" }, { "type": "cds", "sequence": "contig_1", "start": 407548, "stop": 407736, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTTGAAGACTTAACTTCATTATTAACTTTACTACAAGCTGTAGTTGTACTTACAATAGTTGTTATAAAAGCAATAGAAGCTATTGAAGTAATTCAAATTTTTTTATATTTCATAACAATATTCTTTCTTTATTTTTTATTTAAAAAAATTATTAATACCTTTAATTTTAATACACAAAAATAA", "aa": "MSFEDLTSLLTLLQAVVVLTIVVIKAIEAIEVIQIFLYFITIFFLYFLFKKIINTFNFNTQK", "aa_hexdigest": "65b8a3374a31e4d7a68460a9e3ed6c2a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7280.727999999995, "isoelectric_point": 6.013133430480956 }, "id": "GPKLONFDEH_717", "locus": "GCA000006625_00694" }, { "type": "cds", "sequence": "contig_1", "start": 408077, "stop": 408508, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACTTAAAGGTGTTAAAGCAGAAACATTAGCTGCTGAGAATGTAGGTACTTTATTTATTGCAGATTTTAAATTTGGAAATTATAAAACTAATTTTTTAGATAAAAATAAAATAGGTGGAATTGTTATGCAGTCACGATCATCAGGTTTGCCTTCAAAAACACCAGCTTTATTCATGCCTAATTGGTTATTTGGTAAAAATAACCCTTATTTAGAGACTGACGTTAACGCTTCAGCTGGAATTACAGATTTTTTTGTTGATCCATATGATGGCTTAATCTTTACAGGTAAAACCTTAGATCGCATTTATGATGCAAAAAAATTTGATAATGTTAATAAAGCGGTTATTGATGGTAAAAGCAATTTTCAAACATTTACAGATTATGCAAAAGCAATTGTTAGTGTTGTACGAAAGAATGTTTCGAATTGA", "aa": "MELKGVKAETLAAENVGTLFIADFKFGNYKTNFLDKNKIGGIVMQSRSSGLPSKTPALFMPNWLFGKNNPYLETDVNASAGITDFFVDPYDGLIFTGKTLDRIYDAKKFDNVNKAVIDGKSNFQTFTDYAKAIVSVVRKNVSN", "aa_hexdigest": "f2416f7d19ace204e66ea1d46abecc85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15822.848399999983, "isoelectric_point": 9.047122383117674 }, "id": "GPKLONFDEH_718", "locus": "GCA000006625_00695" }, { "type": "cds", "sequence": "contig_1", "start": 408548, "stop": 408763, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATGCCAAACGTAATGAAAGGAGCACGAATTATTGATCCTAATTTTAGTAAAGATGATTTACTATTCTTAGGTAATATTTTTATTCCACAACCTGTTTATTTTCCAATTTTATATGCAGACCAAAATGATGAATATACACCTGGATTAGGAGCTAAATTTCCTATTCCTAAAAAAGATATAAATGAAGTTCAACGCTATGTAGATAATTGA", "aa": "MTMPNVMKGARIIDPNFSKDDLLFLGNIFIPQPVYFPILYADQNDEYTPGLGAKFPIPKKDINEVQRYVDN", "aa_hexdigest": "3e0284f79302b7f13b5f79c4c7f10c41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8148.301800000003, "isoelectric_point": 4.717147636413574 }, "id": "GPKLONFDEH_719", "locus": "GCA000006625_00696" }, { "type": "cds", "sequence": "contig_1", "start": 408833, "stop": 409135, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGAAGCTTTTGAAGGAACGGTTGATAAAGTAATTTATTCATATAATGAATATTTAATTAAAGGATATAAACCAGGAACAGCAGAAGGTGAAGCTATAATTAAAAAATTCGAAGCTAATTTAGCAAAATATGTAAATAGTTTAGATATAAATACAAAAAATAGTTTTAATCCTACAAGGATTTTAAAAGAAAAGCCAGTGGTAGGTAAAAACTTTTTTATCGAAAGAAAAGGTAATATTTACGATGCTAATTATGGATTTATAGGTCAAAAAACTATGGTTAATATTTTAAATCGTTGA", "aa": "MGEAFEGTVDKVIYSYNEYLIKGYKPGTAEGEAIIKKFEANLAKYVNSLDINTKNSFNPTRILKEKPVVGKNFFIERKGNIYDANYGFIGQKTMVNILNR", "aa_hexdigest": "13aee8182c833eb50b9cd42c2f6a97f5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11363.895299999991, "isoelectric_point": 9.272955131530761 }, "id": "GPKLONFDEH_720", "locus": "GCA000006625_00697" }, { "type": "cds", "sequence": "contig_1", "start": 409136, "stop": 409252, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGAAAATAGTCCAGAAGTTGATTTGGGTATTCCTAATTTTACTAAGGATAATGTTAAACATTTACGTGCTTTTAAAGATGAATTAAATGTTAAAAATACTGATGAATAA", "aa": "MNGENSPEVDLGIPNFTKDNVKHLRAFKDELNVKNTDE", "aa_hexdigest": "55e052b34c7ff6e3ace59dff2e0ac26d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4329.713700000001, "isoelectric_point": 4.864417457580566 }, "id": "GPKLONFDEH_721", "locus": "GCA000006625_00698" }, { "type": "cds", "sequence": "contig_1", "start": 409345, "stop": 409518, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTTGAAGTTTAAAAAAATCTTATTATCTATATCAACTAGTTTAATAATTTTATCATCAATTATTACAATTGCTGCATGTAGCAATCAAGTAAAATCTAAAACAAATTTTTCAAAAACAACACCAACTTTAGAAGAATTGACTGAGCTTAAAAAAAGAGAAGAAGCATGA", "aa": "MKLKFKKILLSISTSLIILSSIITIAACSNQVKSKTNFSKTTPTLEELTELKKREEA", "aa_hexdigest": "99ba6db3745f8d2b7a036199857b7604", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6369.532199999998, "isoelectric_point": 9.731776618957518 }, "id": "GPKLONFDEH_722", "locus": "GCA000006625_00699" }, { "type": "cds", "sequence": "contig_1", "start": 409795, "stop": 410232, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTCACAGGTAAAGGAGCATTACCAGAGAATTTTGCTAAATTAAATGTGGGTACATTATTTATTGCTGATTTTAAATTCAATAAGTATAAAGAAAATTTTTTAGATAAAAATGAAATTGGTGGTGTAGCAATGCAATCACGATCATCAGGTTTGCCTTCAAAAACACCAGCCTTATTCATGCCTAGCTGGTTGTTTGGTAAAAATAATCCTTATTTAGAAACTGATGTTAATGCTTCAGCTGGAATTACAGATTTTTTTGTTGATCCATATGATGGCTTAATCTTTACAGGTAAAACCTTAGATCGCATTTATGATGCAAAAAAATTTGATAATGTTAATAAAGCGGTTATTGATGGTAAAAGCAATTTTCAAACATTTACAGATTATGCTAATGTTGCTGTAAAAGCTGTTCGAAAAAGAGTTTCACAATGA", "aa": "MNFTGKGALPENFAKLNVGTLFIADFKFNKYKENFLDKNEIGGVAMQSRSSGLPSKTPALFMPSWLFGKNNPYLETDVNASAGITDFFVDPYDGLIFTGKTLDRIYDAKKFDNVNKAVIDGKSNFQTFTDYANVAVKAVRKRVSQ", "aa_hexdigest": "679c2e3fa5ba039b8d5b8cf6c9c6719e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16143.15389999998, "isoelectric_point": 9.246458625793458 }, "id": "GPKLONFDEH_723", "locus": "GCA000006625_00700" }, { "type": "cds", "sequence": "contig_1", "start": 410272, "stop": 410487, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTCCTGATGTAATGCCAAATGGTCGAATTGTAGATCCAAACATTACTAAAGAAGATTTATTACATTTAAGTAATTTTTTTATTACCCAACCCGTTTATTTACCAATTTTATATGCTGATCAAAATCATCCTTATACACCTGGAATGGGTTGTAAATTCCCTATTCCTAAAAAACACTTAGAAGATGTACAAAAATATATTGATAATTGA", "aa": "MISPDVMPNGRIVDPNITKEDLLHLSNFFITQPVYLPILYADQNHPYTPGMGCKFPIPKKHLEDVQKYIDN", "aa_hexdigest": "305fa4d6dffa1b9915d154a83a15dbd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8182.386199999999, "isoelectric_point": 5.773841285705566 }, "id": "GPKLONFDEH_724", "locus": "GCA000006625_00701" }, { "type": "cds", "sequence": "contig_1", "start": 410560, "stop": 410862, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGAAGCTTTTGAAGGAACGGTTGATAAAGTAATTTATTCATATAATGAATATTTAATTAAAGGATATAAACCAGGAACAGCAGAAGGAGAAGCTGTAATTAAAAAATTCGAAGCTAATTTAGCAAAATATGTAAATAGTTTAGATATAAATACAAAAAATAGTTTTAATCCTACAAGGATTTTAAAAGAAAAGCCAGTTGTTGGTAAAAATTTCTTTATTGAACGTGAAGGAACATTCTGCGATGGTTGTTATGGTTTTATGGGTCAAAAAACAATTGTTAATATTTTAAATCGTTGA", "aa": "MGEAFEGTVDKVIYSYNEYLIKGYKPGTAEGEAVIKKFEANLAKYVNSLDINTKNSFNPTRILKEKPVVGKNFFIEREGTFCDGCYGFMGQKTIVNILNR", "aa_hexdigest": "96237c3c2d659c97e8df4518dd6d31c5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11286.811299999994, "isoelectric_point": 8.51383991241455 }, "id": "GPKLONFDEH_725", "locus": "GCA000006625_00702" }, { "type": "cds", "sequence": "contig_1", "start": 410863, "stop": 410979, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGAAAATAGTCCAGAAGTTGATTTGGGTACTCCTAATTTTACTAAGGATAATGTTAAACATTTACGTGCTTTTAGAGATGAATTAAATGTTAAAAATACTGATGAATAA", "aa": "MNGENSPEVDLGTPNFTKDNVKHLRAFRDELNVKNTDE", "aa_hexdigest": "7b04eebe2de077b3f1328567f83a9b9a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4345.6734000000015, "isoelectric_point": 4.864417457580566 }, "id": "GPKLONFDEH_726", "locus": "GCA000006625_00703" }, { "type": "cds", "sequence": "contig_1", "start": 411137, "stop": 411370, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0Z0", "UniRef:UniRef90_A0AAC9T0Z0" ], "nt": "ATGAAAAATAAGTATTTTAGAAAAAATAAATTTTGGTTATTAATACCAATAAGTTTTGTAAGTCTTGGAAGTATTACATTAGTTGCTACTGCTTGTGTAAAGAAAAATTTTTATCCTAATACATTTGCAGATGATCAGATTTATATCACTAAGAATAATGAAAAAACTGTTAAGTTTCGTATTAGTCGTAATGATGAAGAAAATAAAAAGTATTTTAAAGATGATCTTAAATGA", "aa": "MKNKYFRKNKFWLLIPISFVSLGSITLVATACVKKNFYPNTFADDQIYITKNNEKTVKFRISRNDEENKKYFKDDLK", "aa_hexdigest": "20837a7943271dc80082b53a6b299f09", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 411137, "stop": 411661, "observations": { "mutation": [ 411368 ], "stop": [ 411368 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0Z0", "query_cov": 1.0, "subject_cov": 0.4425287356321839, "identity": 0.935, "score": 146.0, "evalue": 2.31e-43, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MKNKYFRKSKFWLLIPISFIXXXSITLVATACVKKNFYPNTFADDQIYITKNNEKTVKFRISRNDEENKKYFKDDLKWNTFINKLKKTYINVETVIPKENAKQNIKQQDLCMRALPVQVLSNNKYVDIYVKIPVLLANTKMLVVGDDLLKSFYFDTDNLIMNAKHFQPIPIKML" }, "paralog": false, "identity": 0.9655172413793104, "score": 339.0, "evalue": 6.29e-122, "description": "Internal stop codon at 411368. Nonsense mutation around 411368.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0Z0", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T0Z0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0Z0", "UniRef:UniRef50_A0AAC9T0Z0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0Z0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0Z0" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_727", "locus": "GCA000006625_00704" }, { "type": "cds", "sequence": "contig_1", "start": 411470, "stop": 411661, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCGCATTACCTGTACAAGTTTTAAGTAATAATAAATATGTTGATATTTACGTAAAGATTCCTGTTTTATTAGCTAATACTAAAATGTTAGTTGTTGGTGATGATTTACTAAAATCATTTTATTTTGATACAGATAACTTAATAATGAATGCTAAACATTTTCAACCAATTCCTATCAAAATGCTTTAG", "aa": "MRALPVQVLSNNKYVDIYVKIPVLLANTKMLVVGDDLLKSFYFDTDNLIMNAKHFQPIPIKML", "aa_hexdigest": "01708b12d56e12c70f4df3142a577e7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7251.6391, "isoelectric_point": 9.100502204895019 }, "id": "GPKLONFDEH_728", "locus": "GCA000006625_00705" }, { "type": "cds", "sequence": "contig_1", "start": 412003, "stop": 413130, "strand": "-", "frame": 1, "gene": "adhE", "product": "NADP-dependent glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "COG:COG1012", "COG:I", "SO:0001217", "UniRef:UniRef50_Q9PQC9", "UniRef:UniRef90_Q9PQC9" ], "nt": "TTGAACTATAAAACATTAATTAATGGAGCATTTGTTGATGCTAAAGAAAAACTACCAGTTTATAATCCATCTAATAATCAAATTATTGCTTATGTTCCTAACATTCATAATGAAAATGAAATTAATACTATTTTTGAAAATGCTCATATAGCATATCTAAAATTTAAGGACACACCAATTAAATATCGTTGTGATCTACTTTTAAAATTAGCCGATAAACTAGATGAACATAAACAAGAATTAGCACAAATTATTAGTACTGAAATTGCAAAAGGATTAAAAGATAGCCTAATTGAAGTTGAACGAAGTGCTGATTATTTACGCGAAACAGTTTTTGAGTATCAAAAATTAATGCAAAAACCAATTATTTTTGATGAAACAGTTCACCATGTTAAAAACAAAGTGGCAACATATTATCGTATTCCAGTTGGTGTTGTTTTAGCAATTTGTCCTTTTAATTATCCTATTAACTTATTGATTTCAAAATTAGCACCAGCATTAGTTTCTGGTAATAGTTTAGTTTATAAACCATCAACACAAGGTTCATTAATTGGTATTCGAATTAGTGAATTAGTTCATGAAGTAGGTTTTCCAAAAGGAGTTGTTAACTGCTTAACAACTGAAGCACGTATAACAGGTGATCTACTAGTTACTAATAAATATGTTAAAGCGATTAGCTTTACGGGCGGACCTAAAGTAGGCAATCATATTGCTGAAATAACATCAAAGATTTCTTTAGTTTTAGAATTAGGAGGAAAAGATCCAGCCCTTGTTTTAGATGATGCGGATTTTGAACTAGCTGCTAATGAAATAGTTAAAGGTGCTTATGGCTTTAGCGGGCAAAGATGTACTGCAATCAAACGAGTATTTGTTTCGCATAAAAATCATGATTTATTAGTTAATTTAATTAACAAAAAAGTTGACGCTTTAACAGTCGGTTTACCACAACAAAATCCCATAATTACTCCATTAATTAATTCTAATTCATTAAAATATAACTTATCATTAGTTGAGGATGCAATCAAAAAAGGAGCAATAGTACACCAAAAAATCGTTTATAATGAAAAAAATAACCTTTTGCATCCTTTAGTAATTGATAATGTTACAAAAGAAATGCGCGTAGCTTGA", "aa": "MNYKTLINGAFVDAKEKLPVYNPSNNQIIAYVPNIHNENEINTIFENAHIAYLKFKDTPIKYRCDLLLKLADKLDEHKQELAQIISTEIAKGLKDSLIEVERSADYLRETVFEYQKLMQKPIIFDETVHHVKNKVATYYRIPVGVVLAICPFNYPINLLISKLAPALVSGNSLVYKPSTQGSLIGIRISELVHEVGFPKGVVNCLTTEARITGDLLVTNKYVKAISFTGGPKVGNHIAEITSKISLVLELGGKDPALVLDDADFELAANEIVKGAYGFSGQRCTAIKRVFVSHKNHDLLVNLINKKVDALTVGLPQQNPIITPLINSNSLKYNLSLVEDAIKKGAIVHQKIVYNEKNNLLHPLVIDNVTKEMRVA", "aa_hexdigest": "0b4a759e40e44648432dada4dcf8caa6", "start_type": "TTG", "rbs_motif": "AGGAG", "genes": [ "adhE" ], "truncated": "5-prime", "pseudogene": { "start": 411703, "stop": 413130, "observations": { "mutation": [ 412005 ], "stop": [ 412005 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQC9", "query_cov": 1.0, "subject_cov": 0.7894736842105263, "identity": 0.981, "score": 717.0, "evalue": 5.47e-259, "gene_start": 1, "gene_end": 375, "reference_start": 1, "reference_end": 375, "reference_sequence": "MNYKTLINGAFVDAKEKLAVYNPSNNQIIAYVPNIHDENEINTIFENAHKAYLKFKDTTIECRCDLLLKLADKLDKHKQELAQIISTEIAKGLKDSLIEVERSADYLRETVFEYQKLMQKPIIFDETVHHVKNKVATYYRIPVGVVLAICPFNYPINLLISKLAPALVSGNSLVYKPSTQGSLIGIRISELVHEVGFPKGVVNCLTTEARITGDLLVTNKYVKAISFTGGPKVGNHIAEITSKISLVLELGGKDPALVLDDADFELAANEIVKGAYGFSGQRCTAIKRVFVSHKNHDLLVNLINKKVDALTVGLPQQNPIITPLINSNSLKYNLSLVEDAIKKGAIVHQKIVYNEKNNLLHPLVIDNVTKEMRVAWEEPFGPILPIITYNSIQEAIDLINQSQYGLQACIFTTNYASIEQLALQIESGTININKSSSRGPDILPFFGVKDSGFGIQGIVDAILSMTTIKGIIINK" }, "paralog": false, "identity": 0.9789473684210527, "score": 910.0, "evalue": 0.0, "description": "Internal stop codon at 412005. Nonsense mutation around 412005.", "psc": { "uniref90_id": "UniRef90_Q9PQC9", "gene": "AdhE", "product": "NADP-dependent glyceraldehyde-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_Q9PQC9", "cog_id": "COG1012", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQC9", "UniRef:UniRef50_Q9PQC9", "COG:COG1012", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQC9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQC9" ], "product": "NADP-dependent glyceraldehyde-3-phosphate dehydrogenase" } }, "id": "GPKLONFDEH_729", "locus": "GCA000006625_00706" }, { "type": "cds", "sequence": "contig_1", "start": 413213, "stop": 413416, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTCGTGTTAGCAAACAAGATTTTATTAAGCAAACAAATTTTGCTAATTTAAAAAATGATCAAGAAAATAATTTTTATCATCAAATGATTTTAAATAATCAACTACCCCAAACAATTGGTGGGGGAATCGGTCAAAGTCGTTTATGTATGTTTTTATTAAACAAAAAACACATTGGTGAGGTACAAGTTTCAGTTTGA", "aa": "MGIRVSKQDFIKQTNFANLKNDQENNFYHQMILNNQLPQTIGGGIGQSRLCMFLLNKKHIGEVQVSV", "aa_hexdigest": "edb95d7efde8b4eaaecf5bcfae9c6cb6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000314F64C", "query_cov": 1.0, "subject_cov": 0.8170731707317073, "identity": 0.552, "score": 69.3, "evalue": 4.09e-14, "valid": false, "uniref50_id": "UniRef50_A0A352WUX0" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7648.739100000001, "isoelectric_point": 9.42909755706787 }, "id": "GPKLONFDEH_730", "locus": "GCA000006625_00707" }, { "type": "cds", "sequence": "contig_1", "start": 413915, "stop": 414151, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "GTGGATCAATTACAAAAAGCTAAAATTAATCAAACACAAAAAGCAATTGTAGAGATTAAAAATTCTTTTCAAAAACATTTTGCAAAAAATTTAAATCTTTCACGTGTAACAGCACCATTATTTGTTGAAGGACAATCAGGTCTAAATGATCATTTAGACCATAAACAAAAAGCAGTAAGTTTTTATGCTAAAAAATTAGATAAAACACTTGAAATTGTTCAATCACTAGCAAAATGA", "aa": "MDQLQKAKINQTQKAIVEIKNSFQKHFAKNLNLSRVTAPLFVEGQSGLNDHLDHKQKAVSFYAKKLDKTLEIVQSLAK", "aa_hexdigest": "ea6671d2eba639b41fd7de58d1fe233c", "start_type": "GTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 67, "aa_cov": 0.8461538461538461, "hmm_cov": 1.0, "evalue": 5.8208107945000874e-21, "score": 70.86546325683594, "start": 13, "stop": 78 } ], "seq_stats": { "molecular_weight": 8864.154199999994, "isoelectric_point": 9.795277976989745 }, "id": "GPKLONFDEH_731", "locus": "GCA000006625_00708" }, { "type": "cds", "sequence": "contig_1", "start": 414255, "stop": 414785, "strand": "-", "frame": 2, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "db_xrefs": [ "EC:2.7.8.5", "GO:0008444", "GO:0008654", "GO:0016020", "KEGG:K00995", "SO:0001217", "UniRef:UniRef50_Q9PQC7", "UniRef:UniRef90_Q9PQC7" ], "nt": "ATGGCCAAATTGAATTTTAAAAAAAACATCTCATCATCACCTTTTTTTAGAAACATTCCTAATATTATCACTATCTTTAGAATTTTTTTAGCACTTATTTGCATCATCTTATTATTAGTTGATTTTTACACAAAAAATTTAAATATTGTCTATGAAGTTTTAGATGCCAATATTAGTGCATTGCGTTTAAGTGCAACAATTATTTTTATTATTGCTGCTTTTTCTGATTTCTTAGATGGATATATAGCTCGTAAATATAATTTAGTTTCAAATTTAGGGAAAATCTTAGATCCTATTTCTGATAAAATCCTTGTTAATGGTGTTTTAATTTGTTTAACACTAGATCATACAGCACTAGCTTATTTAACAATTATTAATATCTTACGAGATATTTTTATTGATGGTTTACGAATGTTTGCTTCAAGTAAGAAAATTATTATTCCAGCAAATATTTTTGGCAAAATTAAATCCATTCTACTATTTATTAGTATTTGCTTTATTTTATTTTTACTAAGCTTAACCTCATCATGA", "aa": "MAKLNFKKNISSSPFFRNIPNIITIFRIFLALICIILLLVDFYTKNLNIVYEVLDANISALRLSATIIFIIAAFSDFLDGYIARKYNLVSNLGKILDPISDKILVNGVLICLTLDHTALAYLTIINILRDIFIDGLRMFASSKKIIIPANIFGKIKSILLFISICFILFLLSLTSS", "aa_hexdigest": "0e5a90ebcfb42603dac8c9e1b6d3a23d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQC7", "query_cov": 1.0, "subject_cov": 0.8073394495412844, "identity": 0.983, "score": 312.0, "evalue": 2.01e-106, "valid": true, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "ec_ids": [ "2.7.8.5" ], "uniref50_id": "UniRef50_Q9PQC7", "kegg_orthology_id": "K00995", "go_ids": [ "GO:0008444", "GO:0008654", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQC7" ], "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase" }, "genes": [ "pgsA" ], "id": "GPKLONFDEH_732", "locus": "GCA000006625_00709" }, { "type": "cds", "sequence": "contig_1", "start": 414766, "stop": 414879, "strand": "-", "frame": 1, "gene": null, "product": "Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Q3ETT1" ], "nt": "ATGTCATCAGGTTTTGGAATTGGTTTTGAGCGTTTAGTGATGTATGTAACAGGAGTTAATAATATTAAAGATACAATCCCATTCCCACGAAGTCATGGCCAAATTGAATTTTAA", "aa": "MSSGFGIGFERLVMYVTGVNNIKDTIPFPRSHGQIEF", "aa_hexdigest": "7edc3b4addcd3396cfc476fa07e55789", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A7X8F0W0", "query_cov": 0.9459459459459459, "subject_cov": 0.813953488372093, "identity": 0.6, "score": 53.1, "evalue": 1.17e-08, "valid": false, "product": "Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein", "uniref50_id": "UniRef50_A0A4Q3ETT1" }, "pscc": { "uniref50_id": "UniRef50_A0A4Q3ETT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Q3ETT1" ], "product": "Aminoacyl-tRNA synthetase class II (D/K/N) domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_733", "locus": "GCA000006625_00710" }, { "type": "cds", "sequence": "contig_1", "start": 414910, "stop": 415434, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATTGAACCTGAAATGGCTTTTGTTGATTTAAAAGGTATGCAAGATATTGTTGAAGATTTAGTTAAACATGTTATTAAGGCTGTTTTAGAAAAAAATCAACAAGAATTAGAATTTTTAGCTCAAAGAAATGATGAAAATTTAATTAAAAAACTACAAAAAGTTGTTGAGTCTAAATTTGAACGTATTGAATATAAAGATGCAGTTAAAATCCTAGCTGATGCTGTTAAAAATGGTCATCAATTCGAAAATAATGAAATCTTTTTTGGAATGGATTTAGGATCAGAGCACGAAAGATACATGTGTGAAACATATCATCAAGGTCCTGTGTTCTTACAAAATTATCCAAAGGATATTAAAGCATTTTATATGAAATTAAACGATGATCAACAAACTGTAGCTTCAACAGATTTATTAATTCCAGGCGTTGGCGAATTAGTGGGTGGATCACAACGTGAAGATAGTTATGAGAAACTATTAAAACGATGTCAAGAATTAAAAATGCCAATTGAAAGTTTACAATGA", "aa": "MIEPEMAFVDLKGMQDIVEDLVKHVIKAVLEKNQQELEFLAQRNDENLIKKLQKVVESKFERIEYKDAVKILADAVKNGHQFENNEIFFGMDLGSEHERYMCETYHQGPVFLQNYPKDIKAFYMKLNDDQQTVASTDLLIPGVGELVGGSQREDSYEKLLKRCQELKMPIESLQ", "aa_hexdigest": "a64464f5150cb50f2f10fd3f2e8561a5", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 177, "aa_cov": 0.9655172413793104, "hmm_cov": 0.7909604519774012, "evalue": 1.4203562791430478e-26, "score": 88.824462890625, "start": 1, "stop": 168 } ], "seq_stats": { "molecular_weight": 20199.898899999997, "isoelectric_point": 4.9362619400024395 }, "id": "GPKLONFDEH_734", "locus": "GCA000006625_00711" }, { "type": "cds", "sequence": "contig_1", "start": 415435, "stop": 415647, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "TTGTTTACAGGAAATGATGCTGAAGGAGCTGGCGAAACTTTTAGTATTCAAAAATTTGATAATGAAGAATTTTTTGGTAAACAAACTCATTTATCTGTAACAGGTCAATTACAAGCTGAAGCATACGCACAAGCTTTTGGTAATGTTTATACATTTGGTCCTACTTTTAGAGCTGAAAAATCTCACACAAATCGTCATTTAGCAGAATTTTGA", "aa": "MFTGNDAEGAGETFSIQKFDNEEFFGKQTHLSVTGQLQAEAYAQAFGNVYTFGPTFRAEKSHTNRHLAEF", "aa_hexdigest": "fda3433bcbf0d06af3dc5d60d28b2154", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 70, "aa_cov": 0.9714285714285714, "hmm_cov": 0.9285714285714286, "evalue": 7.7180735028487e-09, "score": 30.57632827758789, "start": 3, "stop": 70 } ], "seq_stats": { "molecular_weight": 7824.406400000001, "isoelectric_point": 5.131219673156737 }, "id": "GPKLONFDEH_735", "locus": "GCA000006625_00712" }, { "type": "cds", "sequence": "contig_1", "start": 416053, "stop": 416193, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTTTTGCTTTTTTATTTATATATTATTTAAAAAAGAAAAAAAAGGTAATGATTTTATTATGCAATTAAAAATTAAAGAAATTTTTAATCAAGACTATGTAAAACTTGAAGGCCAAAAGATACAAATTAAAGCTTGA", "aa": "MMFCFFIYILFKKEKKGNDFIMQLKIKEIFNQDYVKLEGQKIQIKA", "aa_hexdigest": "88d04bdda25849c679b9eb60c4b01251", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5651.812400000002, "isoelectric_point": 9.417557716369632 }, "id": "GPKLONFDEH_736", "locus": "GCA000006625_00713" }, { "type": "cds", "sequence": "contig_1", "start": 416371, "stop": 416847, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6V9", "UniRef:UniRef90_A0AAC9X6V9" ], "nt": "ATGTCTGCAACTAATAAAAAATATGATCCACAAATTAGCGAATTATTAGAGCTTCGTGATTTAGCTAATAAAGTTGATAATTTAAAAAGAAAATTAAAAACAATTGAATATAACCATTATTGTGAACACGAAAATTATGATAGTGATGATGGTGAAACTAATAATGTTTATGAACAAGAAGTGTCATTAAATCATGAAACACAAATTGTTAAATTACGTGTTGATTTAAATGATTTAACAAACCTAAGCAATACAAAAATTGTTGAAGTTGAAAAACCTGTTTATATTGAAAAAGTTATTGAAGTTGAAAAGCCAATCGAAAAAATTGTTGAGAAAATTGTTTATGTTAACCAAGATAATCAAGTTAATAAACAAGAAAGTATTAAAGAAGAAAAAGAACCAAAAACACCTGTTAAATCTGGCAAATACGTTAAATATCGTCGTTTAAAAAATAATGGTATGTCAGATGCTGGTTGA", "aa": "MSATNKKYDPQISELLELRDLANKVDNLKRKLKTIEYNHYCEHENYDSDDGETNNVYEQEVSLNHETQIVKLRVDLNDLTNLSNTKIVEVEKPVYIEKVIEVEKPIEKIVEKIVYVNQDNQVNKQESIKEEKEPKTPVKSGKYVKYRRLKNNGMSDAG", "aa_hexdigest": "6ec7f4ec6de758d34ea9cecd8df9c08c", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 416224, "stop": 416859, "observations": { "mutation": [ 416373 ], "stop": [ 416373 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6V9", "query_cov": 1.0, "subject_cov": 0.7488151658767772, "identity": 1.0, "score": 295.0, "evalue": 3.83e-100, "gene_start": 1, "gene_end": 158, "reference_start": 5, "reference_end": 162, "reference_sequence": "MEEQMSATNKKYDPQISELLELRDLANKVDNLKRKLKTIEYNHYCEHENYDSDDGETNNVYEQEVSLNHETQIVKLRVDLNDLTNLSNTKIVEVEKPVYIEKVIEVEKPIEKIVEKIVYVNQDNQVNKQESIKEEKEPKTPVKSGKYVKYRRLKNNGMSDAGWKIFELVKQTQQDKVAAEKRFDEQQEEKKRQREQKRLEKQQKLEAKNEQ" }, "paralog": false, "identity": 0.990521327014218, "score": 377.0, "evalue": 8.41e-135, "description": "Internal stop codon at 416373. Nonsense mutation around 416373.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6V9", "uniref50_id": "UniRef50_A0AAC9X6V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6V9", "UniRef:UniRef50_A0AAC9X6V9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6V9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18470.5683, "isoelectric_point": 5.5386983871459945 }, "id": "GPKLONFDEH_737", "locus": "GCA000006625_00714" }, { "type": "cds", "sequence": "contig_1", "start": 416987, "stop": 417649, "strand": "-", "frame": 3, "gene": null, "product": "DUF2130 domain-containing protein", "db_xrefs": [ "COG:COG4487", "COG:S", "SO:0001217", "UniRef:UniRef50_A0A2C9DYH1", "UniRef:UniRef90_A0A2C9DYH1" ], "nt": "ATGACATTCACTAAAATTAATGAAGCAATTGATGGTAAAAAAGCTGACTTTTTATTAGAATTTTTTGATTTTGGTAAAGAAATGTCAAATGATGATAAAAAATTAATTTTTAGTGCGACAATTGAAGCGAAAACAGAATTTTTTGACAATCAAAAAGGTACAAAAAATAGTGCTCACTATAAAAAATTAGATCAAGATCGTATTAATCAAAAATCTGAATATGCAATTTTAGTAACTGAATTAGAACCAGAAGATCATTTTGTAATTAAAAAAATTAATGAATACAAAAATATGTTTGCTGTACGGCCACAATACTTCATTCCTTTAGTTGATATGATTCGAAATTTTGCAACCCTTAAAGCAAAAATTAATTCACAAATCATAAGATATGAAGATCGTGCAAAAATTGAAGAAAATCTTGATGAATTAAAAAAAGATATTGTTGATAACACTCTAAAATACATCAATGATAAAACTAAAAAAATAATCGATGATTCAAAAGCTATTATTAAAAAAGCAGAAAGTATTGAAGAAAGTGCTGAAGATATTATTAACAAAAAACTAAATACTTTAAAAAAGAAAATCAATGAATTAACAATTAGAAAAATTGATCCAAATAAACTTTTGTTAGAAGTTAGTGATGATGAAAATGATGAAGAATAA", "aa": "MTFTKINEAIDGKKADFLLEFFDFGKEMSNDDKKLIFSATIEAKTEFFDNQKGTKNSAHYKKLDQDRINQKSEYAILVTELEPEDHFVIKKINEYKNMFAVRPQYFIPLVDMIRNFATLKAKINSQIIRYEDRAKIEENLDELKKDIVDNTLKYINDKTKKIIDDSKAIIKKAESIEESAEDIINKKLNTLKKKINELTIRKIDPNKLLLEVSDDENDEE", "aa_hexdigest": "7457f97cfe341da8f41356e348b7f19d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 416987, "stop": 417949, "observations": { "mutation": [ 417697, 417838 ], "stop": [ 417697, 417838 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYH1", "query_cov": 1.0, "subject_cov": 0.4339250493096647, "identity": 1.0, "score": 335.0, "evalue": 1.08e-110, "gene_start": 1, "gene_end": 220, "reference_start": 288, "reference_end": 507, "reference_sequence": "MKTTANKKIKLINIEKLEFEILEPINTNEILVFKDENSTIYESFNQNLEKQRDEFIKKIREEEEQNWRNNFLANDRDYNELVKQRYDLEKQRDELKEKLKNEGNKAIAHFKDSDEYKNLIKAQEKINSLNKTIESNEQNYKKEIENIELKLKSQFDEETKSLKNTIAKQEIKLDNAEKMAIINFKESNEYQKIIKDKIDLDIEIEKLKFAIQAHEDNMKAAKENWESKKIVEIKELESKKDKEIHKLTESIEQLKREKSSNVKLVGEELEQWLKNKFDETYSFSCPDMTFTKINEAIDGKKADFLLEFFDFGKEMSNDDKKLIFSATIEAKTEFFDNQKGTKNSAHYKKLDQDRINQKSEYAILVTELEPEDHFVIKKINEYKNMFAVRPQYFIPLVDMIRNFATLKAKINSQIIRYEDRAKIEENLDELKKDIVDNTLKYINDKTKKIIDDSKAIIKKAESIEESAEDIINKKLNTLKKKINELTIRKIDPNKLLLEVSDDENDEE" }, "paralog": false, "identity": 0.99375, "score": 535.0, "evalue": 7.65e-192, "description": "Internal stop codon at 417697, 417838. Nonsense mutation around 417697, 417838.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYH1", "product": "DUF2130 domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYH1", "cog_id": "COG4487", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYH1", "UniRef:UniRef50_A0A2C9DYH1", "COG:COG4487", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYH1" ], "product": "DUF2130 domain-containing protein" } }, "id": "GPKLONFDEH_738", "locus": "GCA000006625_00715" }, { "type": "cds", "sequence": "contig_1", "start": 417836, "stop": 417976, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTATTAATTTTAAAGAAAGTAATGAATATCAAAAAATTATTAAAGATAAAATAGATTTAGATATTGAAATAGAAAAATTAAAATTTGCTATTCAAGCTCATGAAGATAATATGAAGGCTGCTAAAGAAAATTGA", "aa": "MAIINFKESNEYQKIIKDKIDLDIEIEKLKFAIQAHEDNMKAAKEN", "aa_hexdigest": "2f9c1b1d7265b184e74796f710546256", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5422.191000000001, "isoelectric_point": 5.21272678375244 }, "id": "GPKLONFDEH_739", "locus": "GCA000006625_00716" }, { "type": "cds", "sequence": "contig_1", "start": 418310, "stop": 418510, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACTACAGCTAATAAAAAAATTAAATTAATTAATATTGAAAAATTAGAATTTGAAATTCTTGAGCCGATTAATACCAATGAGATTTTGGTTTTCAAAGATGAAAATTCAACTATTTATGAATCTTTTAATCAAAATTTAGAAAAACAACGTGATGAATTTATCAAAAAAATTCGTGAAGAAGAAGAACAAAATTGA", "aa": "MKTTANKKIKLINIEKLEFEILEPINTNEILVFKDENSTIYESFNQNLEKQRDEFIKKIREEEEQN", "aa_hexdigest": "933471465171ce7e97823a717c86c35d", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8002.967700000002, "isoelectric_point": 4.852367591857909 }, "id": "GPKLONFDEH_740", "locus": "GCA000006625_00717" }, { "type": "cds", "sequence": "contig_1", "start": 418695, "stop": 419690, "strand": "+", "frame": 3, "gene": "deoA", "product": "thymidine phosphorylase", "db_xrefs": [ "COG:COG0213", "COG:F", "EC:2.4.2.4", "GO:0006206", "SO:0001217", "UniRef:UniRef50_A0AAP9AA50", "UniRef:UniRef90_A0AAP9AA50" ], "nt": "GTGTTTTTCTTTGCTAAAATTAAAGATAAGTTTTTAAAAATTAAATTGGTGAAATTTATGTTAAATGTTGTAGAAATTATTAGCAAGAAAAAAAATAATATTGAATTAAGTGAACAAGAAATTAAATTTGTTTATGATGGTTTTGTTAATAAGACAATTCCAGATTATCAAATGGCAGCTTTTTTAATGGCTGTTAATTTTTGTGGATATAGTGAAAATGAACAATACTATGCAACTAAAGCAATGGTTGAATCTGGTAAATTATTAGATTTGCGTGTTGAAAATAAAATTGTTGTTGACAAACATTCATCTGGTGGTGTGGGAGATAAAGTTTCAATTATTTTAACTCCTTTACTATCAGCTTTAGGATTATACGTTGGAAAAATGTCTGGTCGAGGATTAGGGCATACTGGTGGTACAGTTGATAAATTAGAATCATTGAATTTAAATTTAGATTTTGATTTAAAAACATACATGAATCAGCTAAAAGATAATGGTTTATTATTAACTGGTCAATCAGATGATATGGTTGTGGCTGATAAATATATTTATGCATTACGAGATGTAACAGCAACAAGTGATGTGTTTGATCTTATGATAGGATCAATTATGGCTAAAAAATTAGCTTTAATTACTGATTATATTTTCTTAGATGTTAAAGTTGGTGAAGGAGCATTTTGTAAAAATGTTGCACAAGCAGAATCTTTAGCAACAAAGATGTTAAAATTATCAAAGAAATTTAATCGTAATACAATTATCCATTTAACAAATATGGACCAACCTTTAGGAAAAGCAATTGGTAATGCTATTGAAATTAAAGAAAGTATGGATTATTTATTAGGTAAACCAGTGCCCCAAGATTTATATGATTTAATTAATCAGTTTGCTTTAGACATCTTAATTGATACAAAATTCGCTAAAAACAAGGAAGATGCTCAATCTAAGATTGATAACGTCATTAAAAATGGTTTAGCTTATCAAAAATTTGTGAATTGA", "aa": "MFFFAKIKDKFLKIKLVKFMLNVVEIISKKKNNIELSEQEIKFVYDGFVNKTIPDYQMAAFLMAVNFCGYSENEQYYATKAMVESGKLLDLRVENKIVVDKHSSGGVGDKVSIILTPLLSALGLYVGKMSGRGLGHTGGTVDKLESLNLNLDFDLKTYMNQLKDNGLLLTGQSDDMVVADKYIYALRDVTATSDVFDLMIGSIMAKKLALITDYIFLDVKVGEGAFCKNVAQAESLATKMLKLSKKFNRNTIIHLTNMDQPLGKAIGNAIEIKESMDYLLGKPVPQDLYDLINQFALDILIDTKFAKNKEDAQSKIDNVIKNGLAYQKFVN", "aa_hexdigest": "170223ad58bdf324a3d6a7a9fcf2437c", "start_type": "GTG", "rbs_motif": null, "genes": [ "deoA" ], "truncated": "3-prime", "pseudogene": { "start": 418752, "stop": 419993, "observations": { "mutation": [ 419688, 419697 ], "stop": [ 419688, 419697 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAP9AA50", "query_cov": 0.9425981873111783, "subject_cov": 0.7188940092165899, "identity": 0.9740000000000001, "score": 594.0, "evalue": 7.76e-212, "gene_start": 20, "gene_end": 331, "reference_start": 1, "reference_end": 312, "reference_sequence": "MLNVVEIISKKKNNIELSEQEIKFVYDGFVNKTIPDYQMAAFLMAVNFCGYSENEQYYATKAMVESGKVLDLRVENKIVVDKHSSGGVGDKVSIILTPLLSALGLYVGKMSGRGLGHTGGTVDKLESLNLNLDFDLKTYMDQLKDNGLLLTGQSDDMVLADKYIYALRDVTATSDVFDLMVGSIMAKKLALITDYIFLDVKVGEGAFCKNVEQAESLATKMLKLSKKFNRNAIIHLTNMDKPLGKAIGNAIEIKESMDYLLGKPVPQDLYDLINQFALDILIDTKFAKNKEDAQAKIDNVIKNGLAYQKFVNWVRWWYKGDYISLENNTYFNPKYKLEILAKQDGYLDFKSTKELGMIAVDLKAGRKVKTDQLDFQAGIYLNKKNNDFVKTNEVIATLYANQPISDEVVNKYHNNVVYLKTPKQIAPSIIKVMR" }, "paralog": false, "identity": 0.961352657004831, "score": 781.0, "evalue": 3.78e-288, "description": "Internal stop codon at 419688, 419697. Nonsense mutation around 419688, 419697.", "psc": { "uniref90_id": "UniRef90_A0AAP9AA50", "gene": "DeoA", "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0AAP9AA50", "cog_id": "COG0213", "cog_category": "F", "go_ids": [ "GO:0006206" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAP9AA50", "EC:2.4.2.4", "UniRef:UniRef50_A0AAP9AA50", "COG:COG0213", "COG:F", "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AA50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AA50" ], "product": "Thymidine phosphorylase" } }, "id": "GPKLONFDEH_741", "locus": "GCA000006625_00718" }, { "type": "cds", "sequence": "contig_1", "start": 419817, "stop": 420053, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "ATGATTGCTGTTGATTTAAAAGCAGGACGTAAAGTTAAAACTGATCAATTAGATTTTCAAGCTGGAATTTATTTAAATAAGAAAAATAATGATTTCGTTAAAACAAATGAAGTTATTGCTACACTTTATGCTAGCGAGCCAATTAATGATGAAGTTGTTAATAAATACCATAACAATGTAGTTTATTTAAAAACACCAAAACAAATTGCACCATCAATTATTAAAGTAATGCGTTAA", "aa": "MIAVDLKAGRKVKTDQLDFQAGIYLNKKNNDFVKTNEVIATLYASEPINDEVVNKYHNNVVYLKTPKQIAPSIIKVMR", "aa_hexdigest": "998af4fca64e36ae0c62e02bc39e1849", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 63, "aa_cov": 0.7948717948717948, "hmm_cov": 1.0, "evalue": 3.07650631073282e-23, "score": 76.89330291748047, "start": 1, "stop": 62 } ], "seq_stats": { "molecular_weight": 8879.224799999996, "isoelectric_point": 9.435479927062985 }, "id": "GPKLONFDEH_742", "locus": "GCA000006625_00719" }, { "type": "cds", "sequence": "contig_1", "start": 420774, "stop": 421085, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAGAATTGCTTGTATTTCACAAGATGTACCAACTAACTTTGATAGCGATGTTTTTATGCGAATTACAAAAAGTGTTGAACAGTTTTCAGAATATAAATATGACATGAATGAATACTTTCATCCCAATGTTACTCAAAATAAAATTAATTTCGCTTATAAAGTAATTGCAGATCATATGCGTGCAACTGTTTTTGCGATTGCAGATGGTGCAATTCCTTCAAATAAAGAACGAGGTTACATTTTACGTCGTTTAATTCGTCGAACTATGGTTTTAGTGCGTCGTTTAAATATTAACAATCTTTTATGA", "aa": "MERIACISQDVPTNFDSDVFMRITKSVEQFSEYKYDMNEYFHPNVTQNKINFAYKVIADHMRATVFAIADGAIPSNKERGYILRRLIRRTMVLVRRLNINNLL", "aa_hexdigest": "74994e3e4e343d0dcb6d545516f370c3", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12147.918599999985, "isoelectric_point": 9.458753013610842 }, "id": "GPKLONFDEH_743", "locus": "GCA000006625_00720" }, { "type": "cds", "sequence": "contig_1", "start": 421119, "stop": 421499, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGATTTTTATACTTATTTAAAAGATGAAAAAACACTTACTAAAATTAAAATGATTTTAAATAAAGAAGTGCAATTATTTGAAAAAACTTTACAATTAGGTTTAAATATTTTTGAAAATTCTATTCGTAATCAAGAATTAGATAAAGAAATTACTTTTAAACTAGTTGATACATATGGTTTTCCAATTGAATTAATTAAAGAAATTTGTGAACAACGTAATGTTAAAGTTGATTTAGAAGCTTTTGATGCTATGTTTAAACATCACCAATTAATTTCAAAAGCAAACAAAGCTAATTTAAAAGTAATGGAATCACAAAACGAATCACTAATGCAATTAGATGTTGATTCAACCTTTCACTATGAAATATTTAGATGA", "aa": "MGDFYTYLKDEKTLTKIKMILNKEVQLFEKTLQLGLNIFENSIRNQELDKEITFKLVDTYGFPIELIKEICEQRNVKVDLEAFDAMFKHHQLISKANKANLKVMESQNESLMQLDVDSTFHYEIFR", "aa_hexdigest": "0f0e16c7f6e80f4a963438e17e5c6a73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14957.109299999987, "isoelectric_point": 5.403762626647949 }, "id": "GPKLONFDEH_744", "locus": "GCA000006625_00721" }, { "type": "cds", "sequence": "contig_1", "start": 421542, "stop": 422186, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "TTGGTTGATGGTTTAGATCATGAAGATGGTTATGTTGTTTTTGATAACACATGTTTTTATGCAACATCAGGTGGTCAACAACATGACACAGGTTATATTATTAAAAATGATCAACAATTTTTTGTTGATGATGTTTTTAAGGCACCTAATCGTCAACACGTTCATCATGTTAAAAATGCTAGTTTATCAATGAATGAGCATGTTATTTTACAAATCAACGAGCAAGATCGTAAATCAATTACAGCAAACCATACAGCCGAACACTTATTACACTATTGCTTAAAACATGTTTTAAGTCCTGATATTAAACAAGAAGGAGCTGCTAAATATCCACATAAAGTAACATTTGATTTTACTTATCATGCTCAACCAACAAAAGCACAATTAGATAAGTTAGAAAATGTATTAAATGAAATGGTACAAAGTAATTTTGATGTACAAGAATTACACATGGATTTAGATGAAGCTAAAGCTGTTGGTGCTGCTGCTTATTTTGAAGATGTTTATAAAAAATTAAAAGGCAAATTACGTGTAATTAAAATGGGACCTTCAATTGAGTTATGTGGGGGTACACACGCACATCATACATCAGAAATCGAGCGAATTAAAATTGTTGAATGTGCTTCTAAAGGGGCTGGCTCTTGA", "aa": "MVDGLDHEDGYVVFDNTCFYATSGGQQHDTGYIIKNDQQFFVDDVFKAPNRQHVHHVKNASLSMNEHVILQINEQDRKSITANHTAEHLLHYCLKHVLSPDIKQEGAAKYPHKVTFDFTYHAQPTKAQLDKLENVLNEMVQSNFDVQELHMDLDEAKAVGAAAYFEDVYKKLKGKLRVIKMGPSIELCGGTHAHHTSEIERIKIVECASKGAGS", "aa_hexdigest": "5b0f9839d6b2a43fb8b460d4a426e744", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 39, "aa_cov": 0.17757009345794392, "hmm_cov": 1.0, "evalue": 4.004644004475962e-13, "score": 44.776092529296875, "start": 176, "stop": 213 } ], "seq_stats": { "molecular_weight": 24114.864300000012, "isoelectric_point": 6.026604270935058 }, "id": "GPKLONFDEH_745", "locus": "GCA000006625_00722" }, { "type": "cds", "sequence": "contig_1", "start": 422196, "stop": 422336, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACAGGTCATGATAATTTAGCAAAATACATTCATGATTTATATGTAGACTACTTAAATGAAATCAATCACTTAAAAGTTAATTTAGATATTAATGATCATAAATTAAATGATTTATATAATGCTTTTGCAAATTGA", "aa": "MVTGHDNLAKYIHDLYVDYLNEINHLKVNLDINDHKLNDLYNAFAN", "aa_hexdigest": "b89af7ef66a012b7bd590b0ccc1474f2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5414.969100000001, "isoelectric_point": 5.193401527404784 }, "id": "GPKLONFDEH_746", "locus": "GCA000006625_00723" }, { "type": "cds", "sequence": "contig_1", "start": 422797, "stop": 423240, "strand": "+", "frame": 1, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "COG:COG0816", "COG:JLK", "EC:3.1.-.-", "EC:3.1.21.10", "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364", "RefSeq:WP_006688766.1", "SO:0001217", "UniParc:UPI00000C1C3F", "UniRef:UniRef100_Q9PQC1", "UniRef:UniRef50_Q9PQC1", "UniRef:UniRef90_Q9PQC1" ], "nt": "ATGCGTAAATTAGCACTTGATTTAGGAACAAAATCATGTGGTTTTGCAATTAGTGATTTATTAGGAATAATTGCTAGCGGACTTGATAATTTTATTTATGAAGAAAATGATTTTACAGCTGTTTTAGCTAAAATTGATGAAATCATGATTAATTATCATCATGAAATTGACACAATTGTTTTAGGTTATCCAACTAATGTTTATGATGGATCAAAAAATGAACGAACATATTTAATCGAATCATTTTATGCATTATTAAAACAACACTTTTTAAACCATGAAAAAATTAAAATTGTTTATGAGGATGAACGTTTTAGCACTAAAATTGCAACTCAACGTTTAAAAAATAGTTGTGTTAAAGCAGCTAAAATCAAAAAAGTTAAAGATAAAATGAGTGCTGTTGTTATTTTAGAAAGTTATTTAAGTAAAAATCATTTTAATTAA", "aa": "MRKLALDLGTKSCGFAISDLLGIIASGLDNFIYEENDFTAVLAKIDEIMINYHHEIDTIVLGYPTNVYDGSKNERTYLIESFYALLKQHFLNHEKIKIVYEDERFSTKIATQRLKNSCVKAAKIKKVKDKMSAVVILESYLSKNHFN", "aa_hexdigest": "9595dd16b96bdfcbab5ef58b05874f83", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C3F", "ncbi_nrp_id": "WP_006688766.1", "uniref100_id": "UniRef100_Q9PQC1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C3F", "RefSeq:WP_006688766.1", "UniRef:UniRef100_Q9PQC1" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQC1", "uniref90_id": "UniRef90_Q9PQC1" }, "psc": { "uniref90_id": "UniRef90_Q9PQC1", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.-.-", "3.1.21.10" ], "uniref50_id": "UniRef50_Q9PQC1", "cog_id": "COG0816", "cog_category": "JLK", "go_ids": [ "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQC1" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "GPKLONFDEH_747", "locus": "GCA000006625_00724" }, { "type": "cds", "sequence": "contig_1", "start": 423367, "stop": 423489, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCATATCCTAGTGGAAAAGGATTGCATGTTGGTCATCCCAAAGGTTATACAGCAACTGATGTAATTAGTCGTTTTAAACGTTTGAATGGTTATGATGTTTTACATCCAATTGGTTGA", "aa": "MFPYPSGKGLHVGHPKGYTATDVISRFKRLNGYDVLHPIG", "aa_hexdigest": "64ecca8a855b65eb3eeedf0a466144ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4427.052000000001, "isoelectric_point": 9.696576881408692 }, "id": "GPKLONFDEH_748", "locus": "GCA000006625_00725" }, { "type": "cds", "sequence": "contig_1", "start": 423958, "stop": 424230, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "GTGCAATTTAAGATTAAAGATTCACTTTTAACACTTGATGTGTTCACTACACGAATTGATACTATTTATGGGGTACAATACTTAGTAGTTGCACCAGAACATCCAATTCTTAAATCTATTACTAGTGAGCAACAAATTAATGTTGTTCAATCATATATTGAACAAACAAAGAAAATAAGCGATTTAGATCGCATTGCTGATACTAATAAAACTGGTGTTTTTAGTGGTGCATATGCTATTAATCCAATCAATCAAGAAATCATTCCAATTTGA", "aa": "MQFKIKDSLLTLDVFTTRIDTIYGVQYLVVAPEHPILKSITSEQQINVVQSYIEQTKKISDLDRIADTNKTGVFSGAYAINPINQEIIPI", "aa_hexdigest": "f0f91ebcae212ebd6b88e0458c2491e7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 91, "aa_cov": 1.0, "hmm_cov": 0.978021978021978, "evalue": 1.0956384958742752e-25, "score": 85.79299926757812, "start": 1, "stop": 90 } ], "seq_stats": { "molecular_weight": 10154.55549999999, "isoelectric_point": 5.145486259460449 }, "id": "GPKLONFDEH_749", "locus": "GCA000006625_00726" }, { "type": "cds", "sequence": "contig_1", "start": 424249, "stop": 424506, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "ATGAATTTTGCAACTGGAGCTGTTATGGGTGTACCAGCACATGATGAACGTGATTATGCTTTTGCTAAAAAATATGCATTACCAATTAAAAGTGTAATTGATACAAAACAAAAATTACCATATGCAGGTGATGGTTTACATATTAACTCAGCAATGATTAATGGTTTAAATATTAAACAATCTCAAAATGTTTTAAATGATTATTTAATTAAAAATCATCTAGGAAAAAAAGTTGCAAACTATAAATTGCGTAATTGA", "aa": "MNFATGAVMGVPAHDERDYAFAKKYALPIKSVIDTKQKLPYAGDGLHINSAMINGLNIKQSQNVLNDYLIKNHLGKKVANYKLRN", "aa_hexdigest": "b3dd206d459e8ed854000a70cf842c9a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 76, "aa_cov": 0.8, "hmm_cov": 1.0, "evalue": 1.2749737643578195e-19, "score": 66.01909637451172, "start": 1, "stop": 68 } ], "seq_stats": { "molecular_weight": 9459.846799999992, "isoelectric_point": 9.69799518585205 }, "id": "GPKLONFDEH_750", "locus": "GCA000006625_00727" }, { "type": "cds", "sequence": "contig_1", "start": 424909, "stop": 425112, "strand": "+", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A359BCU9", "UniRef:UniRef90_UPI0013027DF7" ], "nt": "TTGTATGATATTGGTGTAGTGCCAACAAAAGAACCATTTTATAAAGTAATTAATCAGGGAATGATTTTAGGCGAAAATAATGAAAAAATGTCTAAATCAAAAGGGAATGTTATTAATCCTGATGATATTATTGCTTCGCATGGTGCTGATACATTAAGAATTTATGAAATGTTTATGGGACCATTAACAGCTTCATTACCATGA", "aa": "MYDIGVVPTKEPFYKVINQGMILGENNEKMSKSKGNVINPDDIIASHGADTLRIYEMFMGPLTASLP", "aa_hexdigest": "465a1d6a46a0429b3dc5455b6205cba3", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 424813, "stop": 425178, "observations": { "mutation": [ 424828, 424897, 425110, 425143 ], "stop": [ 424828, 424897, 425110, 425143 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013027DF7", "query_cov": 0.9850746268656716, "subject_cov": 0.4520547945205479, "identity": 0.848, "score": 120.0, "evalue": 1.01e-33, "gene_start": 1, "gene_end": 66, "reference_start": 37, "reference_end": 102, "reference_sequence": "LASPEKLKKWLPVDVYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFMKLFNQGMILGENNEKMSKSKGNVDNPDDIVESHGADTLRLYEMFMGPLDASIAWSETGLDGARRFLDRVWRLFTNEDGSLSDKVTEQSGGALERSY" }, "paralog": false, "identity": 0.7520661157024794, "score": 187.0, "evalue": 2.34e-62, "description": "Internal stop codon at 424828, 424897, 425110, 425143. Nonsense mutation around 424828, 424897, 425110, 425143.", "psc": { "uniref90_id": "UniRef90_UPI0013027DF7", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A359BCU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013027DF7", "UniRef:UniRef50_A0A359BCU9" ] } }, "id": "GPKLONFDEH_751", "locus": "GCA000006625_00728" }, { "type": "cds", "sequence": "contig_1", "start": 425305, "stop": 425676, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "ATGATGGTTTTTGTTAATGTTTTATACAAAAATAAAGTTATTAATTATGAATTATTAGATAATTTTTTAATTTTACTTTCATGCTATGCACCACATTTAGCGGAGGAATTATATAGTTTAAACCATTCAGAATCTGTTTGTTTACAAAAAATGCCTATTTATGATGAACAAAAAATTATTGCTCAAAATATTACAATTCCAATTCAAATTAATGGTAAATTAAAGCACACAATCAATGTTTTAAGAGATACAAATGCTGAAGAGTTAGTTAATTTAGCATTGGCTTGTGAACAAGTTAAACAAGAAATTGGGGATCAACCAATTAAAAAACAAATCGTTGTTGTTAATAAAATTATTAATTTTGTTATTTAA", "aa": "MMVFVNVLYKNKVINYELLDNFLILLSCYAPHLAEELYSLNHSESVCLQKMPIYDEQKIIAQNITIPIQINGKLKHTINVLRDTNAEELVNLALACEQVKQEIGDQPIKKQIVVVNKIINFVI", "aa_hexdigest": "c48d6dbbaf60da6ac87373d881a57622", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 83, "aa_cov": 0.5934959349593496, "hmm_cov": 0.9879518072289156, "evalue": 1.6067459141124846e-13, "score": 46.368812561035156, "start": 15, "stop": 87 } ], "seq_stats": { "molecular_weight": 14122.495699999981, "isoelectric_point": 5.873877906799316 }, "id": "GPKLONFDEH_752", "locus": "GCA000006625_00729" }, { "type": "cds", "sequence": "contig_1", "start": 425876, "stop": 426493, "strand": "-", "frame": 3, "gene": "insO", "product": "Integrase-recombinase protein", "db_xrefs": [ "COG:COG2801", "COG:X", "SO:0001217", "UniRef:UniRef50_C4XED4", "UniRef:UniRef90_Q9PQB9" ], "nt": "ATGAATAAATTGGGTTTGCTATGCGAAATAAGAAGAAGCAAAAGAAAAAGAGAAATAAAGAATGTTAATGCAACATTCAAAAACTTAGCCAATAGAGATTATGATGGCCAAATGAATGATATATATGCTACCGATGTGACATATATTCCTTCACCCACTGATGTTATAGAAAATCATGTATATTTATCGGTTTTATTGCATCATAAAACAAAGAAAATTGTGAGCTGGAATTTATCAAAATATAATGATACTAATCTTGTTTTAAAACATTTTAAACTAACTAAATTTCCTAAAAAATTCATTGTACATTCAGATCACGGATATCAATATTCCTCTAAGGAATTCCTTGATTACATAGATTCAATTAATGGCACTATTTCTATGTCAAGAGTTGGAAATTCTTTGGATAATAGAGAAGTAGAATATTTCTTTTCTATTCTAAAAACTGAAATATTTTCAAATTTTTTTCAAAAAGCAAAAAGTTTAACATTTAATGAATTGCAAATAGAAATAGACAAATTTATTAATTTGTATAATAATGAAAGATTTATCAAAAAATTTGATTTAAAAACACCCCAACAAATGTGGGATGTTTATATGAACAATAAATCTATTTGA", "aa": "MNKLGLLCEIRRSKRKREIKNVNATFKNLANRDYDGQMNDIYATDVTYIPSPTDVIENHVYLSVLLHHKTKKIVSWNLSKYNDTNLVLKHFKLTKFPKKFIVHSDHGYQYSSKEFLDYIDSINGTISMSRVGNSLDNREVEYFFSILKTEIFSNFFQKAKSLTFNELQIEIDKFINLYNNERFIKKFDLKTPQQMWDVYMNNKSI", "aa_hexdigest": "bb7012bde3baa3c9dba79373e231b800", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_Q9PQB9", "query_cov": 1.0, "subject_cov": 0.9903381642512077, "identity": 0.966, "score": 400.0, "evalue": 7.87e-141, "valid": true, "gene": "InsO", "product": "Integrase-recombinase protein", "uniref50_id": "UniRef50_C4XED4", "cog_id": "COG2801", "cog_category": "X" }, "pscc": { "uniref50_id": "UniRef50_C4XED4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED4" ], "product": "Integrase catalytic domain-containing protein" }, "genes": [ "insO" ], "id": "GPKLONFDEH_753", "locus": "GCA000006625_00730" }, { "type": "cds", "sequence": "contig_1", "start": 426486, "stop": 426644, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_232459371.1", "SO:0001217", "UniParc:UPI000169D3F3", "UniRef:UniRef100_UPI000169D3F3", "UniRef:UniRef50_UPI000169D3F3", "UniRef:UniRef90_UPI000169D3F3" ], "nt": "TTGCCAGCCAAAAACAAGGCAATTCAATATGAAAAACAAATAAAAGAGGCTTTTATTGAATGCAAAGGAAGATTTGGAAGGGAAAGACTTTCACTTTTTTTAAAAGAAAAAGTACCAAATCGATATAAATCCAAGGACTCTGGGCAGAATTATGAATAA", "aa": "MPAKNKAIQYEKQIKEAFIECKGRFGRERLSLFLKEKVPNRYKSKDSGQNYE", "aa_hexdigest": "2d5f698bcbc4d7e17fe63d811ebb09ac", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI000169D3F3", "ncbi_nrp_id": "WP_232459371.1", "uniref100_id": "UniRef100_UPI000169D3F3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000169D3F3", "RefSeq:WP_232459371.1", "UniRef:UniRef100_UPI000169D3F3" ] }, "ips": { "uniref100_id": "UniRef100_UPI000169D3F3", "uniref90_id": "UniRef90_UPI000169D3F3" }, "psc": { "uniref90_id": "UniRef90_UPI000169D3F3", "uniref50_id": "UniRef50_UPI000169D3F3" }, "pscc": { "uniref50_id": "UniRef50_UPI000169D3F3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000169D3F3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6196.082, "isoelectric_point": 9.721655082702636 }, "id": "GPKLONFDEH_754", "locus": "GCA000006625_00731" }, { "type": "cds", "sequence": "contig_1", "start": 426713, "stop": 426817, "strand": "-", "frame": 3, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C41", "UniRef:UniRef100_Q9PQB8", "UniRef:UniRef50_Q9PQB8", "UniRef:UniRef90_Q9PQB8" ], "nt": "ATGTCAAGAGAACAACTTGAATATCTCATTACTGATATTTTTTATGAAGAAATTTTAAAACGCAACAATAGCAAAAATTTAGAAGAAGTTGTTGAAAAAAATTAA", "aa": "MSREQLEYLITDIFYEEILKRNNSKNLEEVVEKN", "aa_hexdigest": "09e3cdbab7465aa002f7715dd52c6690", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C41", "uniref100_id": "UniRef100_Q9PQB8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C41", "UniRef:UniRef100_Q9PQB8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQB8", "uniref90_id": "UniRef90_Q9PQB8" }, "psc": { "uniref90_id": "UniRef90_Q9PQB8", "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PQB8" }, "pscc": { "uniref50_id": "UniRef50_Q9PQB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQB8" ], "product": "Unique hypothetical" }, "genes": [], "id": "GPKLONFDEH_755", "locus": "GCA000006625_00732" }, { "type": "cds", "sequence": "contig_1", "start": 427313, "stop": 428527, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTAGGTGTTACCTTAGTTGGAGCTGGAATAGTTGCTATAGCAGCTTCATGTTCTAATTCAACTGTTAAATCTAAGTTAAGTAACCAATTTGCTAAATCAACAGACGGTAAAAGTTTTTATGCGGTTTACGAAATTGAAAACTTTAAAGATCTAAGTAATGATGATAAAAAATCATTAAGTAACATTGAATTTAATGCTGCACTTACATCAGCTGAAAACAAAACAGAAAGTACACTTGAAAAAGGTCATTTAGTTGGTGAAAAAATTTACGTTAAATTACCTCGTGAACCAAAACCTAATGAACAATTAACTATTATTAGTAAAAGTGGATTAATCAAGACTTCAGGTTTGTTAATATCTGATAATTTGAATTATCAAACAGAAAAAGTGAACTTTGAAACTACACAACCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAACAACCAGCAGGTAAAGAAACTACTGGAAAATAA", "aa": "MTLGVTLVGAGIVAIAASCSNSTVKSKLSNQFAKSTDGKSFYAVYEIENFKDLSNDDKKSLSNIEFNAALTSAENKTESTLEKGHLVGEKIYVKLPREPKPNEQLTIISKSGLIKTSGLLISDNLNYQTEKVNFETTQPGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKEQPAGKETTGK", "aa_hexdigest": "2e82851c5f08e77bf559357af1e8537a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 42067.03529999993, "isoelectric_point": 7.576603507995606 }, "id": "GPKLONFDEH_756", "locus": "GCA000006625_00733" }, { "type": "cds", "sequence": "contig_1", "start": 428871, "stop": 429083, "strand": "+", "frame": 3, "gene": null, "product": "Secreted protein", "db_xrefs": [ "RefSeq:WP_012316984.1", "SO:0001217", "UniParc:UPI000165F8D1", "UniRef:UniRef100_A0A2C9DY03", "UniRef:UniRef50_A0A2C9DY03", "UniRef:UniRef90_A0A2C9DY03" ], "nt": "TTGAATTATCAAACAGAAAAAGAATTAACAACTGCTAAAACTGATGTTGAAAGCAAACAAAAAGTTGCTGATGCTGCTAAAACAAAATTAGATGAAGCAACTAAAGAATCAAAAAACGCAAATCAATTATTAGAAACAGCTAAAACTAATCAAACAACAAAACAAGAAGCAGTTGATAAAGCTAAAGAAGAATTAGAAGAAGCTAAAAAATAA", "aa": "MNYQTEKELTTAKTDVESKQKVADAAKTKLDEATKESKNANQLLETAKTNQTTKQEAVDKAKEELEEAKK", "aa_hexdigest": "845b2826afbf65059080fbdd26e23884", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8D1", "ncbi_nrp_id": "WP_012316984.1", "uniref100_id": "UniRef100_A0A2C9DY03", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8D1", "RefSeq:WP_012316984.1", "UniRef:UniRef100_A0A2C9DY03" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DY03", "uniref90_id": "UniRef90_A0A2C9DY03" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DY03", "product": "Secreted protein", "uniref50_id": "UniRef50_A0A2C9DY03" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DY03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY03" ], "product": "Secreted protein" }, "genes": [], "id": "GPKLONFDEH_757", "locus": "GCA000006625_00734" }, { "type": "cds", "sequence": "contig_1", "start": 429225, "stop": 429974, "strand": "-", "frame": 2, "gene": null, "product": "putative protein UU376", "db_xrefs": [ "RefSeq:WP_012316982.1", "SO:0001217", "UniParc:UPI000165F8D0", "UniRef:UniRef100_A0A2C9DXY4", "UniRef:UniRef50_Q9PQB5", "UniRef:UniRef90_Q9PQB5" ], "nt": "TTGAATTATCAAACAGAAAAAGTGAACTTTGAAGCAGCTTCAGCTCCAAGCCAACAACCATCTCCGACTCCAGCTCCAAAAACTGAAGAACCAAAAGAAAATGGTGGTAGCGAACAGCAAGCTGATCAACCAGAAAATTCTAAAAAAGATGATGTTGTAGCTAGTGGTGTAAAAATTAGTGAAGTCAAAAATAATACTGCTACTATTGAAGTTACATTTAGTAAATTTGAATTAGCAGATGCTAATAAAAAAGATTTTGTTTTAGAAGTTATTAAGAAAGCTGATACAAATCCAATTCAAGCAAGTGATTTAAAATATGATGAAGCATCTAAAACATTAAGCGGTAAACTAAGTGGTTTAAATTCAAATGTTGACTATGAAATTTCAAAACTAACTTTAAATGGTAAAGAAGTCAAATTTAATGAAGAAGAGTTGTTAAAATCATATGTTAAAAATGCAAAATTATTTATGACATTCAACAAAGAAAATAAGAAAATTAATGTTAAATTACAAAATTTTGATATTTTAAATTCTTTAGAAGAAAATCAACCTATATTAACATTTGATATTGAAATTAAAAAAGATAGTAGTTTACAAGTAGTTCATAAATCATTAACAAAGAATCAATTATCAAATCTTAATGGTTTAGAAATTGATTTAAAAGATAAAATGAATGGAAATAATGCAAATTATGATGTTAAATTAACCAATGCTAAATTGTTAAATGTTAACATAGAAACACAAAAATAA", "aa": "MNYQTEKVNFEAASAPSQQPSPTPAPKTEEPKENGGSEQQADQPENSKKDDVVASGVKISEVKNNTATIEVTFSKFELADANKKDFVLEVIKKADTNPIQASDLKYDEASKTLSGKLSGLNSNVDYEISKLTLNGKEVKFNEEELLKSYVKNAKLFMTFNKENKKINVKLQNFDILNSLEENQPILTFDIEIKKDSSLQVVHKSLTKNQLSNLNGLEIDLKDKMNGNNANYDVKLTNAKLLNVNIETQK", "aa_hexdigest": "8cf01ecd9319052bc39a6c69c35a265e", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8D0", "ncbi_nrp_id": "WP_012316982.1", "uniref100_id": "UniRef100_A0A2C9DXY4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8D0", "RefSeq:WP_012316982.1", "UniRef:UniRef100_A0A2C9DXY4" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DXY4", "uniref90_id": "UniRef90_Q9PQB5" }, "psc": { "uniref90_id": "UniRef90_Q9PQB5", "product": "Uncharacterized protein UU376", "uniref50_id": "UniRef50_Q9PQB5" }, "pscc": { "uniref50_id": "UniRef50_Q9PQB5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQB5" ], "product": "Uncharacterized protein UU376" }, "genes": [], "id": "GPKLONFDEH_758", "locus": "GCA000006625_00735" }, { "type": "cds", "sequence": "contig_1", "start": 430144, "stop": 430341, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACAAAGAATGCATTATTTAAAAAGATTGTAAAAATTTCTGATCAATTATTAAATAAAATTAGTATCAAAAAATTAACTAAAGATAAAAAAAATAATTTATTTGTTTATTTTGACGAATTTGTTGATGTAACAATTATTGATGAATTACATCAATTATCAAAAACAACATTAATGCATGATTTACAAATTTGA", "aa": "METKNALFKKIVKISDQLLNKISIKKLTKDKKNNLFVYFDEFVDVTIIDELHQLSKTTLMHDLQI", "aa_hexdigest": "424a6f1cb1ad27ce5e6be2c81619bea2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7679.9888999999985, "isoelectric_point": 8.964731788635255 }, "id": "GPKLONFDEH_759", "locus": "GCA000006625_00736" }, { "type": "cds", "sequence": "contig_1", "start": 430804, "stop": 431676, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGAATAATGCAAAAATTCGAGCTTATGTTTTTTTAAAAAAGATTGATATATTAAAAAGTGGAGCAATAGCTTATAAACTAAATGTTATTGATGATAGTGAAACCTTAACAATTATGACTTATTTACCAAGCGGTGAACATCCTTTAAAAAAGTTTTTAGATGAACTGAAAATTGATCAATTAATCGAGGCTGAAATTGATATTGTTTTAGATAATATGAGTAAGAGTGGTCAAGTTCCTATTGGTAAAATTAAAAAAATTTGTTGCGTTGAAGATAAACATGTAAAAAAACAAATCACACCTCGTTTAGAACTAAATTTTCATACAAAAATGTCATCACTTGATGCAATTATTTCGACACAAGAATTAATTGATTTTGCGGTTAAGAATCAATTAAAAACAATTGGCATCACTGATCGAAATGTTGTACAAGCATATCCTGAAATCGCTAAATTTTCTAAAAAACAAGATTTAAAAATTATTTATGGTTTAGAAACAGAAGAATTAGAAGATCAAATCCCATTAGTTTTAAATGTTCGGGATCAAAATTTAGATAATGCTACATATGTTATTTTTGATATTGAAACAACAGGATTATTTCCTAATTTTGATGAAATTATTGAATTTGGGGCCGTGATTATGCAAAATAATAAGCAAATTGGGGAAAAAATTCAATTTTTTATAAAACCTATTCAACAAATTAATGAGAATGTTACTAATCTAACTAATATTAGTCAAGAAATGGTCAATAATGCAATTGATGAAAAAACAGCATTATTAAAAATTAAAGAAATTTTTGATGACCATATTTTAGTTGCACATAATGGAATTAATTTTGATATTAATTTTATTAATCAGAGACTGTTAAAATGA", "aa": "MNNAKIRAYVFLKKIDILKSGAIAYKLNVIDDSETLTIMTYLPSGEHPLKKFLDELKIDQLIEAEIDIVLDNMSKSGQVPIGKIKKICCVEDKHVKKQITPRLELNFHTKMSSLDAIISTQELIDFAVKNQLKTIGITDRNVVQAYPEIAKFSKKQDLKIIYGLETEELEDQIPLVLNVRDQNLDNATYVIFDIETTGLFPNFDEIIEFGAVIMQNNKQIGEKIQFFIKPIQQINENVTNLTNISQEMVNNAIDEKTALLKIKEIFDDHILVAHNGINFDINFINQRLLK", "aa_hexdigest": "66e15c1c44162f688a8f715a381c6a2a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 99, "aa_cov": 0.3413793103448276, "hmm_cov": 0.7878787878787878, "evalue": 1.2693457871473812e-07, "score": 27.336830139160156, "start": 190, "stop": 288 }, { "id": "PF00929.31", "name": "RNase_T", "length": 104, "aa_cov": 0.3413793103448276, "hmm_cov": 0.9903846153846154, "evalue": 2.4056985975820347e-25, "score": 85.56636810302734, "start": 190, "stop": 288 } ], "seq_stats": { "molecular_weight": 33161.05470000001, "isoelectric_point": 5.284230232238768 }, "id": "GPKLONFDEH_760", "locus": "GCA000006625_00737" }, { "type": "cds", "sequence": "contig_1", "start": 431716, "stop": 431955, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATCACGAGCAATAAATCCATTTAAAAGTCATCGATTAGGAGCGATTTGTAAAAAATATGAAGTTGATTATAATGATGAAAGTGCACACCGTGCAGACTATGATGCCATAGTTTTAGCTGATGTTTTTAAAGTTATGAAAAATAACTTATTTAATGATTTTGGAATTACTAATTTAAGTGAAATTAATACTAAATTACAAACAACAATGTTAAAAAATCGTAGTTTTGGTAATTGA", "aa": "MISRAINPFKSHRLGAICKKYEVDYNDESAHRADYDAIVLADVFKVMKNNLFNDFGITNLSEINTKLQTTMLKNRSFGN", "aa_hexdigest": "18814a64b70ab0afbfadc870a9b72c61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9058.235099999994, "isoelectric_point": 8.818839836120606 }, "id": "GPKLONFDEH_761", "locus": "GCA000006625_00738" }, { "type": "cds", "sequence": "contig_1", "start": 431995, "stop": 432537, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGAATTAGTGTCTATATCTCATACTGATATGTATTATACAAGACCAACAATTACGACAAGCTTTTTAGCAAATAAAAAAGATAAATTAATTATTTCTAACTCGATTCACGAATCAGATTTAATCAATGCTTTATATTCAAAAAACGATGAAGAAATTAAAAGGTTAATTCAACGTTATGATTTTATAACATTACCATCATTAGGTTCACAAAAACACTTAGTATATGCTAAAAAAATTACAATTGAAAATGTACAAAAAGCTTTTAAAAAATTGATCTATTTAGCTTTAGAATTAAACAAGATTATTATTTATTCAAGCTCGCCTTATTATTTCTTTAAAGATGATAAAAAATTTTATGATGTTTATGTTAATACGAAAGGTCTCGAAGGTAAAGCACACCGATTTGCTAATGAAGTTTATGTTCCTGATTTAGAATATATTGATCAAAAAAATGCCATTGATGAATTAGCTTATTTAGAAGATGAAAAGTTAATTAACTTGATTATTAATGAGAATCCTGTGCATATTAATAGTTGA", "aa": "MYELVSISHTDMYYTRPTITTSFLANKKDKLIISNSIHESDLINALYSKNDEEIKRLIQRYDFITLPSLGSQKHLVYAKKITIENVQKAFKKLIYLALELNKIIIYSSSPYYFFKDDKKFYDVYVNTKGLEGKAHRFANEVYVPDLEYIDQKNAIDELAYLEDEKLINLIINENPVHINS", "aa_hexdigest": "547ec0a38ed7205273fb3ff6557502fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21115.935400000002, "isoelectric_point": 6.214002418518065 }, "id": "GPKLONFDEH_762", "locus": "GCA000006625_00739" }, { "type": "cds", "sequence": "contig_1", "start": 432586, "stop": 432732, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07733.18" ], "nt": "ATGGAAGGTGTTGATCAAAAAACAATCGATTATGTTTATCATACTGCTAAAAAAATATATGGAGAGAATTTACCAACAATTGTTGAACAGCGCATTAAAAAAGAATTAAATTCAATTATTAAACATGGTTTTAGTGTTGTTTATTGA", "aa": "MEGVDQKTIDYVYHTAKKIYGENLPTIVEQRIKKELNSIIKHGFSVVY", "aa_hexdigest": "3a1dab87c775e6a09ded7d963af2442e", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 40, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 2.2537736353644866e-07, "score": 26.284317016601562, "start": 9, "stop": 48 } ], "seq_stats": { "molecular_weight": 5597.3994999999995, "isoelectric_point": 8.026136589050292 }, "id": "GPKLONFDEH_763", "locus": "GCA000006625_00740" }, { "type": "cds", "sequence": "contig_1", "start": 432760, "stop": 433353, "strand": "+", "frame": 1, "gene": null, "product": "DNA polymerase III polC-type", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A180EXR3" ], "nt": "ATGCAAGATGGTTATGGTGTTGGTAGTCGTGGTTCGGTTGGCTCATCACTTGTCGCAACGTTTTTAAATATTACTGACGTTAATCCGCTTACACCACATTATTTATGTCCTAATTGTAAAAAATGTGAATTTATAACAAACGCTGATGATGGATTTGATTTAGCTCCTAAAAGTTGTGAGCAGTGTCAAACTCCAATGTTAACTGATGGACATAATATTCCTTTTGAAACTTTTTTAGGTTTTGATGGGGATAAAGTACCAGATATTGATCTTAATTTTTCTGGAGTTTATCAAGCAGTTGCACATAATTTTATTAAAAGTATTTTTGGAGAAACACATTCTTATCGTGCTGGTACAATTGGTACTATGGCCCAAACAAGCGCTGAAAATACGGTTAAGAAGTATTTTGAAAACCGTTTTAATGAAAATAAAATTATTCGTGATTCAACGGTTAGTTTATATGTACAAAAGTGCATTGATTCTAAACGCACAACTGGTCAACATCCTGGGGGTATTATTATTGTTCCTAAAGAATATAGTATTTGGGATTTTTCACCTTATAATTTTCCAGCTAATGATATTAATGAAACTTGA", "aa": "MQDGYGVGSRGSVGSSLVATFLNITDVNPLTPHYLCPNCKKCEFITNADDGFDLAPKSCEQCQTPMLTDGHNIPFETFLGFDGDKVPDIDLNFSGVYQAVAHNFIKSIFGETHSYRAGTIGTMAQTSAENTVKKYFENRFNENKIIRDSTVSLYVQKCIDSKRTTGQHPGGIIIVPKEYSIWDFSPYNFPANDINET", "aa_hexdigest": "13ba336ba48f4c53f2d3fe8615352afd", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A180EXR3", "query_cov": 0.9847715736040609, "subject_cov": 0.8326180257510729, "identity": 0.619, "score": 248.0, "evalue": 1.38e-80, "valid": false, "product": "DNA polymerase III polC-type", "uniref50_id": "UniRef50_A0A180EXR3" }, "pscc": { "uniref50_id": "UniRef50_A0A180EXR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A180EXR3" ], "product": "DNA polymerase III polC-type" }, "genes": [], "id": "GPKLONFDEH_764", "locus": "GCA000006625_00741" }, { "type": "cds", "sequence": "contig_1", "start": 433483, "stop": 433689, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGTATGATCCATTAGTTATGAAATCATTTAGCGATATTAGTGCGCTAAATATTAAACCGTCTGATGTTTTAAATGAAACAACAGGAGCAATTTCTATTCCTGAATTTGGGACACGCTTTGTACGTGGTATGCTAGTGGATACTAAACCAAAATCATTTGCTGACTTAATTCGTATTTCAGGATTATCACATGGAGAAAGTGTTTGA", "aa": "MYDPLVMKSFSDISALNIKPSDVLNETTGAISIPEFGTRFVRGMLVDTKPKSFADLIRISGLSHGESV", "aa_hexdigest": "fc2ba38691c2d0004987e5fb1118893d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 65, "aa_cov": 0.9558823529411765, "hmm_cov": 0.7692307692307693, "evalue": 5.008738442496137e-12, "score": 41.05399703979492, "start": 3, "stop": 67 } ], "seq_stats": { "molecular_weight": 7401.45, "isoelectric_point": 5.608780860900879 }, "id": "GPKLONFDEH_765", "locus": "GCA000006625_00742" }, { "type": "cds", "sequence": "contig_1", "start": 433726, "stop": 433890, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "TTGCTAAAGGATGTTATTGCTTGTCGTGATGATATTATGACATATTTAATTCGTCAAAATGTTGAACCAAAAACTGCCTTTTTAATTATGGAAGATGTTAGAAAAGGTAAAAAGATCAAACCAGAACACCAAATCATTTTAAAAGAATTAAAGGTTCCTGAATGA", "aa": "MLKDVIACRDDIMTYLIRQNVEPKTAFLIMEDVRKGKKIKPEHQIILKELKVPE", "aa_hexdigest": "078d8c3ba034593f920e08e45a29ee70", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 54, "aa_cov": 0.7962962962962963, "hmm_cov": 0.9814814814814815, "evalue": 8.453639122619662e-07, "score": 24.0496768951416, "start": 2, "stop": 44 } ], "seq_stats": { "molecular_weight": 6379.6242999999995, "isoelectric_point": 8.852105522155764 }, "id": "GPKLONFDEH_766", "locus": "GCA000006625_00743" }, { "type": "cds", "sequence": "contig_1", "start": 434176, "stop": 434472, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14579.12" ], "nt": "GTGATTGAATTATTAGCTCGAGGTTTTAAAATTTCAAATATTAGTATTGAACAATCACAGGCGACTAGTTATGTAATTGATAAAGAAAACAATGCTATTATTCCACCATTTATTGCGATTCAAGGATTGGGTGAAACAGTAGCTAATTCAATCATTGAAGCTCGTAATCAAAAAGTTTTTTCAACAATTGAGGATTTAAAAAACCGTACAAAAATTTCGCGTACAGATTTAAAAAATTTACGAGTATTAGGCGTTTTAGATCATTTAAGTGAAACTGAACAACTAACATTATTTTAA", "aa": "MIELLARGFKISNISIEQSQATSYVIDKENNAIIPPFIAIQGLGETVANSIIEARNQKVFSTIEDLKNRTKISRTDLKNLRVLGVLDHLSETEQLTLF", "aa_hexdigest": "d552a72f953817a53c4397f038bb1022", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14579.12", "name": "HHH_6", "length": 89, "aa_cov": 0.8673469387755102, "hmm_cov": 0.9662921348314607, "evalue": 1.5484205912055912e-16, "score": 55.97451400756836, "start": 8, "stop": 92 } ], "seq_stats": { "molecular_weight": 10985.493199999986, "isoelectric_point": 5.7753190994262695 }, "id": "GPKLONFDEH_767", "locus": "GCA000006625_00744" }, { "type": "cds", "sequence": "contig_1", "start": 434587, "stop": 434841, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAAATAAAAAGATTTTGTTTTCTTCATTGTTATTTGGAACTGTAGCTATTGGAACTGTTGCAGTTGCAACTGCATGTAGTGACAATAAGAAAACTAAAAAAACAATCAACACAGGCACTACTCAACCCGGAAGTAGTACAGGAACAACAAGTAAATCATTAACGCAAGAAAAAGTGTTAAGAAATGAAATCATTTCTGAAATTTTGAATGCTAAATCTAAAAAACCAGATCGTGATAAGATGTGA", "aa": "MKKINKKILFSSLLFGTVAIGTVAVATACSDNKKTKKTINTGTTQPGSSTGTTSKSLTQEKVLRNEIISEILNAKSKKPDRDKM", "aa_hexdigest": "c0aca3e8da32b14abe18dbfee7e8140b", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9043.448699999994, "isoelectric_point": 10.130384635925292 }, "id": "GPKLONFDEH_768", "locus": "GCA000006625_00745" }, { "type": "cds", "sequence": "contig_1", "start": 434893, "stop": 435042, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAGCAGTTGATTTAACAAAATCTTCAAATTTTAAAAAATTAATTGCTGATGGAAAATACAAATCTACATTTAGTGCACAAATTGAAGATATTATTACTAAAGTTAAACATGATATTAAAATGTATGCAGAATCACCATTTTGA", "aa": "MDKAVDLTKSSNFKKLIADGKYKSTFSAQIEDIITKVKHDIKMYAESPF", "aa_hexdigest": "617f39365e40223e8e342c26f6fa5421", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5582.403399999999, "isoelectric_point": 9.005153465270997 }, "id": "GPKLONFDEH_769", "locus": "GCA000006625_00746" }, { "type": "cds", "sequence": "contig_1", "start": 435118, "stop": 435447, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTAACATCAATTGATTTACCTGCTGATTTTCCAATTATTTATTCAAAACCTGATTATGATGGTATTCCTGGATTAGGTGCACGTTTTCCAACACCTAAATCAAAAGTAGCTCCTGAAAAATTATTAGATGATGGTTTCTCATTTGGAGATATTATTGTTGAAGATGGTTCAGATACTCAAAAAGCAAATCTACCTGGTCAATTAATTGAATCATTTGAAAAGACAGCTGATAAAGTAATTTACTTATACTATGATTCAGGTTTACCAGAAGCGTTTAAAAACAATCAACGTCAACCTGAAAAAATTAAACAATTTGAAGACTGA", "aa": "MNVTSIDLPADFPIIYSKPDYDGIPGLGARFPTPKSKVAPEKLLDDGFSFGDIIVEDGSDTQKANLPGQLIESFEKTADKVIYLYYDSGLPEAFKNNQRQPEKIKQFED", "aa_hexdigest": "5c1ad5b4f5a24e83ccdf18432d25a4dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12159.481199999991, "isoelectric_point": 4.49666919708252 }, "id": "GPKLONFDEH_770", "locus": "GCA000006625_00747" }, { "type": "cds", "sequence": "contig_1", "start": 435448, "stop": 435546, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAGTCAAAATGCTAATGATTTCGTTGCAAAACGCATGTTAAAAAATCCAAATAATAAGGACGATTTAATTGTTATGCCAATGAGTAGTTTATGA", "aa": "MKSQNANDFVAKRMLKNPNNKDDLIVMPMSSL", "aa_hexdigest": "29695fa9393432f717efd3689f2669b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3651.2629000000006, "isoelectric_point": 9.522705650329591 }, "id": "GPKLONFDEH_771", "locus": "GCA000006625_00748" }, { "type": "cds", "sequence": "contig_1", "start": 435610, "stop": 435780, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAATCTGAATTAGATGCTTTAAAAGCTAAAGAAGAATTTAAAGTGCCAAAACAATTAGTTACATTAGTTAACCAAGCTACAGATTTAAAAGAAGATAAACAAACAATTAAAGATGAATGCGATCCTTTTAAATCACACCGTGATCCAAATCATAAAATTGACTGA", "aa": "MPKSELDALKAKEEFKVPKQLVTLVNQATDLKEDKQTIKDECDPFKSHRDPNHKID", "aa_hexdigest": "99e94d505b94ff4f1ff39ba4d34567a5", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6491.320700000002, "isoelectric_point": 6.071052360534667 }, "id": "GPKLONFDEH_772", "locus": "GCA000006625_00749" }, { "type": "cds", "sequence": "contig_1", "start": 436015, "stop": 436194, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTCTAGCTGGAATTCATGGGGAAGCTGCAACAAAAATGTTTGGTGCATTAATGAAAGAACATAAAGAAATTGCCAATTTTGCAATTACTAACCGCCGATTTAATGATGAAACAAGAAAAGTAGTTGCTCCTCAAGATCGCGATAAATACTCTCAAAATGCAGCTCTTGTTGATTGA", "aa": "MILAGIHGEAATKMFGALMKEHKEIANFAITNRRFNDETRKVVAPQDRDKYSQNAALVD", "aa_hexdigest": "11a7d099d408d39e880d15345292e4f9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6633.510600000003, "isoelectric_point": 8.141212654113772 }, "id": "GPKLONFDEH_773", "locus": "GCA000006625_00750" }, { "type": "cds", "sequence": "contig_1", "start": 436547, "stop": 436822, "strand": "-", "frame": 3, "gene": null, "product": "Conserved hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQB2", "UniRef:UniRef90_Q9PQB2" ], "nt": "ATGGCTAACGTGATTCATAATATCAAAGAATTACAAACGTTAACCATTAACAAAAAAATTAAAATTAAAGATTTTGATTACTTATCTTATTTACCTTTGGTAAAAGATGATCCATTAGCTTCATATTTTAAATCTATTTATGAACAAGAAGTAAAAAGAACTAAACTTGTTTTGGCTCACAATGATTTTTCAATTAATAACATTTTAGCAAATGAATGCGAAAATCTTTTTATATTAATTGATTTTGAATATAGTAGTAGAAATTTAAAAGATTGA", "aa": "MANVIHNIKELQTLTINKKIKIKDFDYLSYLPLVKDDPLASYFKSIYEQEVKRTKLVLAHNDFSINNILANECENLFILIDFEYSSRNLKD", "aa_hexdigest": "b709704140ecf10c0490a1f21c0d87b2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 436307, "stop": 436858, "observations": { "mutation": [ 436411, 436549, 436828 ], "stop": [ 436411, 436549, 436828 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQB2", "query_cov": 1.0, "subject_cov": 0.4972677595628415, "identity": 1.0, "score": 182.0, "evalue": 3.95e-57, "gene_start": 1, "gene_end": 91, "reference_start": 13, "reference_end": 103, "reference_sequence": "MSKNPYVINEWFMANVIHNIKELQTLTINKKIKIKDFDYLSYLPLVKDDPLASYFKSIYEQEVKRTKLVLAHNDFSINNILANECENLFILIDFEYSSRNLKDWDLFNFLRDLNDVQLNFGISFIKEKLGYDECLIYKYLFLTSYYGFLWATNAFLKTNELKIKIYGENAYQKADFYLQKLNK" }, "paralog": false, "identity": 0.9836065573770492, "score": 358.0, "evalue": 3.6e-129, "description": "Internal stop codon at 436411, 436549, 436828. Nonsense mutation around 436411, 436549, 436828.", "psc": { "uniref90_id": "UniRef90_Q9PQB2", "product": "Conserved hypothetical", "uniref50_id": "UniRef50_Q9PQB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQB2", "UniRef:UniRef50_Q9PQB2" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQB2" ], "product": "Conserved hypothetical" } }, "id": "GPKLONFDEH_774", "locus": "GCA000006625_00751" }, { "type": "cds", "sequence": "contig_1", "start": 437111, "stop": 437254, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATGTAAATGGAATGCTGAAAAAATTACGAGCAAGTATTCAAAAAAGTTTTTATTATTATGGTGCAATTAATCAAGATCAAATGCGTTTAAATCAATTTGTTAATGAATTAATAAAATATTTTAAAAATCAAAATTAA", "aa": "MNNVNGMLKKLRASIQKSFYYYGAINQDQMRLNQFVNELIKYFKNQN", "aa_hexdigest": "bd4753f8c72a90616a269d751e84a41c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5677.4968, "isoelectric_point": 9.90023250579834 }, "id": "GPKLONFDEH_775", "locus": "GCA000006625_00752" }, { "type": "cds", "sequence": "contig_1", "start": 437420, "stop": 437617, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAACATTAGTTTTATACGCACGCGTTTGTCCAACTAAATCGTTTCATACACAACAAATTTTTGAAGCTTTTCAAAAACAAACAAATTTTGTTTGTGTTAATCTTGATGCAAATCAACCTTTTGATGTTTCAAAAGAACAAGAATTTATTTTAGGGTTTGATCGAATTATTTTATTGTTTACGCTAAATTGA", "aa": "MKKTLVLYARVCPTKSFHTQQIFEAFQKQTNFVCVNLDANQPFDVSKEQEFILGFDRIILLFTLN", "aa_hexdigest": "5539e9c4a2641ce28a59d447372f8258", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7628.800799999997, "isoelectric_point": 7.795726585388184 }, "id": "GPKLONFDEH_776", "locus": "GCA000006625_00753" }, { "type": "cds", "sequence": "contig_1", "start": 437785, "stop": 439233, "strand": "-", "frame": 1, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "COG:COG0322", "COG:L", "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "SO:0001217", "UniRef:UniRef50_Q9PQA9", "UniRef:UniRef90_Q9PQA9" ], "nt": "ATGATTACAAATGAACAATATCCCCGTTTAAAATATGTTCGTACTTATGATAAAAATAAAGGAATTTATTTTGGGCCATTAGCTGATAGCACAAATAAATATCAATTATTTAATTTATTAAATAGTATTTTTCCATTTAATAAATGTAATCATCAACCATATAAAAAATGTATTTATTATGACCTGCATCAATGTATTAATCAAGTTCAACAATCAACATATCAAGAAGCAATCAGTGAAGTTAAAGAAATCTTTAAAGGTAATTTAGATCACATTTTAATGATTCTACAAACTAAAGAACAACATGCAGTTACTAAATTAGATTTTGAAAATGCTCAAAAATACGCTGAGCAACAAAAAGCACTTACTTCAATTATTAATTCAGGATTAGTGCAATTGGATAATAATGAAAGTTTCGATATCATCGGTTTTTATGAAAAAAATAATTATTTAGTAATTATAATTTTTAATTATGTTAAAGGCAAATTATTAAATAAAAGTGCTGATACATTTGCCATTTATGATTATGAAATTAATGAATTAATAACCTCATTTTTAATGCAATACTATTCACAAAATAAAATTAGTTCAAAAATTATTGTAAGTTTAGATGATGATAATTTATTAGCTTTATCACAACGTTTTCACACAAAATTTATTAATGCGCAAACAAAATTTCATAAACGAATTCTTAAATTAGCTCTTGATAATGCTATTTTGTATTTTGAATCTAATATTAAAAATGTAATTAATAAACAAAATGAATTAGATGATGCTTTAAATCAATTAAAACAAATTTTAAAATTACCAGATTTAAATATCATTGAGTGTTTTGATAATTCTAATATTAATTTAAGTTTACCAATTGCTGGGATGATTGTTTATCAAAACGGTAAATTAAATAACAAATTAAATCGTAAATATAATTTAATGACAACTAAAAATGCTTCTGATTATCATTTTATGATTGAAGTTATCACACGACGTTATCAACGATTAGTTTCACAACATCAAAAATTACCTAATCTAATTGTAGTAGATGGGGGAAAGTTACAAGTTAATGCAGCTTTATATGCGTTAGCACAGTTACAAATAAACATTCCATTAATAGGACTAAAAAAAGATCAAAAACACAAAACTGATGCCATTGTTTTAACTAATGGCGATGAAATTGTTTTAGATCGTAAAAGTATTTTATATAAATTCTTAGCAAATATGCAAAATGATGTTCACAATTATGCAATTTCGTTTTTAAGAGATAAACACACAAAATCTATTTTTAATTCTTTATTAGATAATGTTCAAGGTCTAGGTAAAAAACGTTTTAATGAACTTTTAAAATATTATGATAGCATTAATGATTTAAAAAGTGCATCTGATCAAGAATTATTACAATTTTTACCTAAAAATGTCTTAGTTAATTTACGTGAAAAACTAAATAAAATATAA", "aa": "MITNEQYPRLKYVRTYDKNKGIYFGPLADSTNKYQLFNLLNSIFPFNKCNHQPYKKCIYYDLHQCINQVQQSTYQEAISEVKEIFKGNLDHILMILQTKEQHAVTKLDFENAQKYAEQQKALTSIINSGLVQLDNNESFDIIGFYEKNNYLVIIIFNYVKGKLLNKSADTFAIYDYEINELITSFLMQYYSQNKISSKIIVSLDDDNLLALSQRFHTKFINAQTKFHKRILKLALDNAILYFESNIKNVINKQNELDDALNQLKQILKLPDLNIIECFDNSNINLSLPIAGMIVYQNGKLNNKLNRKYNLMTTKNASDYHFMIEVITRRYQRLVSQHQKLPNLIVVDGGKLQVNAALYALAQLQINIPLIGLKKDQKHKTDAIVLTNGDEIVLDRKSILYKFLANMQNDVHNYAISFLRDKHTKSIFNSLLDNVQGLGKKRFNELLKYYDSINDLKSASDQELLQFLPKNVLVNLREKLNKI", "aa_hexdigest": "abc80b6fe4be013572bb0e083bc7ff77", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQA9", "query_cov": 1.0, "subject_cov": 0.8253424657534246, "identity": 1.0, "score": 946.0, "evalue": 0.0, "valid": true, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_Q9PQA9", "cog_id": "COG0322", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQA9" ], "product": "UvrABC system protein C" }, "genes": [ "uvrC" ], "id": "GPKLONFDEH_777", "locus": "GCA000006625_00754" }, { "type": "cds", "sequence": "contig_1", "start": 439539, "stop": 439850, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20618.3", "PFAM:PF07479.19" ], "nt": "ATGCAACTAATTTTAGAGAAAATTTTTCACAAAAAAGTCAATCCGTTAGAATACTTTTTTATTGGTGATACAATTTTAACTTGTACTGATCAAAAATCACGTAATTTTAGTTTTGGTTTACTGATTGCACAACAAGGCGTACAAGAAGCTTTAGTAAATAAACAACAGACTATTGAAGGTTTAAATAACATCAAAGTTATTTATGAAATTATTAAAACAAATCAAATTAGTGCTCCACTTTTCCAAAATTTATATAAGGTAATTATTAACGCCTTAAATCCCCAGGATTTATTTGATAAAAGTTTTTGTTAA", "aa": "MQLILEKIFHKKVNPLEYFFIGDTILTCTDQKSRNFSFGLLIAQQGVQEALVNKQQTIEGLNNIKVIYEIIKTNQISAPLFQNLYKVIINALNPQDLFDKSFC", "aa_hexdigest": "002fc72abc73396cfd709e30ac8b49e3", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20618.3", "name": "GPD_NAD_C_bact", "length": 64, "aa_cov": 0.6116504854368932, "hmm_cov": 0.984375, "evalue": 8.928690090562255e-18, "score": 60.26945114135742, "start": 21, "stop": 83 }, { "id": "PF07479.19", "name": "NAD_Gly3P_dh_C", "length": 97, "aa_cov": 0.9320388349514563, "hmm_cov": 1.0, "evalue": 5.59606551748699e-22, "score": 74.07715606689453, "start": 3, "stop": 98 } ], "seq_stats": { "molecular_weight": 11865.703899999984, "isoelectric_point": 7.74643840789795 }, "id": "GPKLONFDEH_778", "locus": "GCA000006625_00755" }, { "type": "cds", "sequence": "contig_1", "start": 439998, "stop": 440513, "strand": "-", "frame": 2, "gene": "gpsA", "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0240", "SO:0001217", "UniRef:UniRef50_Q9PQA8", "UniRef:UniRef90_Q9PQA8" ], "nt": "ATGAGTAAAATTTTAATTGTTGGAAGCGGAGCTTTTGGAAGTGCTTTAACACAAGTTTTAGTTTCAAATTCTCATATTGTTGATGTTTATGGAATTAATGAAAATGAGCTTAATGATTTGAAAGAAAATCAAAAAAATAAGAAATATTTTCAGGATCAAAAATTAGATTTACCAATTAATAATACATATTTGGATATTAATTTAGCACTTCAGAATCAATATGATTTTATCATCATTGCTATTCCTTCATTTGCTATTAAAAACTTTGTTGATAATATCAAAATTTTTAATTTATCAAAAACTATTGTTGTTAATGCAGCGAAAGGATTAAATCTTGAAACAAAATCATCATGGTGTGATTATATTAAACAAAATTTAAAAATTAAAGCCTTAGTTGGATTAGTTGGCCCTTCATTTGCAATTGATGTTTTTTTAAAAAAACCAACAGTCGTTAATTTAGTAGGCAATAATTCAAACGCCCTTATAAAAGTTAAAGAAGAATTTGAAAACAATTGA", "aa": "MSKILIVGSGAFGSALTQVLVSNSHIVDVYGINENELNDLKENQKNKKYFQDQKLDLPINNTYLDINLALQNQYDFIIIAIPSFAIKNFVDNIKIFNLSKTIVVNAAKGLNLETKSSWCDYIKQNLKIKALVGLVGPSFAIDVFLKKPTVVNLVGNNSNALIKVKEEFENN", "aa_hexdigest": "91e3b947e82abdc7a08ffaeb7e3dfc8b", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [ "gpsA" ], "truncated": "5-prime", "pseudogene": { "start": 439695, "stop": 440513, "observations": { "mutation": [ 440000 ], "stop": [ 440000 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQA8", "query_cov": 1.0, "subject_cov": 0.5277777777777778, "identity": 1.0, "score": 327.0, "evalue": 7.05e-111, "gene_start": 1, "gene_end": 171, "reference_start": 1, "reference_end": 171, "reference_sequence": "MSKILIVGSGAFGSALTQVLVSNSHIVDVYGINENELNDLKENQKNKKYFQDQKLDLPINNTYLDINLALQNQYDFIIIAIPSFAIKNFVDNIKIFNLSKTIVVNAAKGLNLETKSSWCDYIKQNLKIKALVGLVGPSFAIDVFLKKPTVVNLVGNNSNALIKVKEEFENNWFKCILSKQFDAANYISCFKNALAIGCGIIYGLQNSNNSLVAFLTKGINEMQLILEKIFHKKVNPLEYFFIGDTILTCTDQKSRNFSFGLLIAQQGVQEALVNKQQTIEGLNNIKVIYEIIKTNQISAPLFQNLYKVIINALNPQDLFDKSFC" }, "paralog": false, "identity": 0.9963235294117647, "score": 533.0, "evalue": 1.98e-194, "description": "Internal stop codon at 440000. Nonsense mutation around 440000.", "psc": { "uniref90_id": "UniRef90_Q9PQA8", "gene": "GpsA", "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_Q9PQA8", "cog_id": "COG0240", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQA8", "UniRef:UniRef50_Q9PQA8", "COG:COG0240", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQA8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQA8" ], "product": "Glycerol-3-phosphate dehydrogenase" } }, "id": "GPKLONFDEH_779", "locus": "GCA000006625_00756" }, { "type": "cds", "sequence": "contig_1", "start": 440554, "stop": 440799, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14714.11" ], "nt": "ATGCGCAAAATTGCAACTTCATTATTAAATGATGTTGTTATTAAAGCAAATGCTTTTCAAGAACCACCACCATTTAAAGGAGGGAGAATCAGTATTTCATATATCGTTCAAGTACAATCACAAATCCCTACTTTTGTTTTAAAATGTAATAACCCTAAATTTTTGCACTTTAGCTATGCTCGATATATTGAAAATGAAATTCGTAAAGCTTTTGGATTTGATAGTGTACCAATAACATTATATTGA", "aa": "MRKIATSLLNDVVIKANAFQEPPPFKGGRISISYIVQVQSQIPTFVLKCNNPKFLHFSYARYIENEIRKAFGFDSVPITLY", "aa_hexdigest": "c2452a01fbd26ed9438cef0d62e8a230", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14714.11", "name": "KH_dom-like", "length": 78, "aa_cov": 0.9629629629629629, "hmm_cov": 1.0, "evalue": 7.928131377868425e-29, "score": 95.4348373413086, "start": 3, "stop": 80 } ], "seq_stats": { "molecular_weight": 9280.728599999997, "isoelectric_point": 9.664987373352051 }, "id": "GPKLONFDEH_780", "locus": "GCA000006625_00757" }, { "type": "cds", "sequence": "contig_1", "start": 440965, "stop": 441696, "strand": "-", "frame": 1, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "SO:0001217", "UniRef:UniRef50_P75309", "UniRef:UniRef90_Q9PQA7" ], "nt": "ATGTTGATTGATACAGGAGGTATAATTAGCTCAAAAGATACTTATCAAGATAACATCAATGAACAAGTTTTATTTGCGATTAATGAAGCAAATACAATTATTTTTTTAGTCTCAGCAAAAGATGGAATTAATAATGATGATAAAAAAATAGCTAAAATGTTAAAAGAAAAAGCTAAAGATAAAAAAATTATTTTAGTTATAAATAAGATCGAATCAGAAAAATATTATCTTAATGAAGGAGAACTATATTCGTTTGGGTTTGGTAAATTTTTTAAAATCTCAGCTGAGCATGGAATAGGAATGGGTGATCTTTTAGATGAATTAGTAAAAGATATGCCTATACAAAATAATTTAGAAAAACAAGAACGCTTTAAATTTTGCATTATTGGAAGACCAAATGTTGGTAAAAGTTCACTAACAAATACCATCTTAGGTGAGCAAAGAGTAATTGTTAATGCTGAAGCAGGTTCAACACGTGATTCAATCGATAATGATTTTAATTACTATAATAAAAAATATACAATCATTGACACTGCAGGAATACGACGAAAAGGTAAAATTGTCGAATCTGTTGAAAAATATGCTGTATTACGAACTAAAAAAGCAATTGAAAGATCCCAATTAATTTTATTAGTACTTGATGGATCTGAACCTTTTAAAGAACAAGATGAAGTTGTTGGTGGTTTAGCATATAATGCAAATATTCCAACAATTATTATTGTTAATAAATGA", "aa": "MLIDTGGIISSKDTYQDNINEQVLFAINEANTIIFLVSAKDGINNDDKKIAKMLKEKAKDKKIILVINKIESEKYYLNEGELYSFGFGKFFKISAEHGIGMGDLLDELVKDMPIQNNLEKQERFKFCIIGRPNVGKSSLTNTILGEQRVIVNAEAGSTRDSIDNDFNYYNKKYTIIDTAGIRRKGKIVESVEKYAVLRTKKAIERSQLILLVLDGSEPFKEQDEVVGGLAYNANIPTIIIVNK", "aa_hexdigest": "02a0a1d136977c5a2ca769740a9a05a0", "start_type": "ATG", "rbs_motif": null, "genes": [ "der" ], "truncated": "both", "pseudogene": { "start": 440662, "stop": 441849, "observations": { "mutation": [ 440886, 440967, 441714 ], "stop": [ 440886, 440967, 441714 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQA7", "query_cov": 1.0, "subject_cov": 0.5497737556561086, "identity": 1.0, "score": 467.0, "evalue": 3.35e-163, "gene_start": 1, "gene_end": 243, "reference_start": 52, "reference_end": 294, "reference_sequence": "MRTIAIVGKPNVGKSSLFNRILMRRKSIVDDQPGVTRDRIYDIGNWLTRSFMLIDTGGIISSKDTYQDNINEQVLFAINEANTIIFLVSAKDGINNDDKKIAKMLKEKAKDKKIILVINKIESEKYYLNEGELYSFGFGKFFKISAEHGIGMGDLLDELVKDMPIQNNLEKQERFKFCIIGRPNVGKSSLTNTILGEQRVIVNAEAGSTRDSIDNDFNYYNKKYTIIDTAGIRRKGKIVESVEKYAVLRTKKAIERSQLILLVLDGSEPFKEQDEVVGGLAYNANIPTIIIVNKWDNIINKNSHTMEMVKKQIRSQFKYLSWAPIVFVSALDNKRIHTIFEAIEFVREQAMRKIATSLLNDVVIKANAFQEPPPFKGGRISISYIVQVQSQIPTFVLKCNNPKFLHFSYARYIENEIRKAFGFDSVPITLYWQDKNKKLRGE" }, "paralog": false, "identity": 0.9924050632911392, "score": 756.0, "evalue": 9.01e-280, "description": "Internal stop codon at 440886, 440967, 441714. Nonsense mutation around 440886, 440967, 441714.", "psc": { "uniref90_id": "UniRef90_Q9PQA7", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75309", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQA7", "EC:3.6.5.-", "UniRef:UniRef50_P75309", "COG:COG1160", "COG:J", "KEGG:K03977", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P75309", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75309" ], "product": "GTPase Der" } }, "id": "GPKLONFDEH_781", "locus": "GCA000006625_00758" }, { "type": "cds", "sequence": "contig_1", "start": 441712, "stop": 441849, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02421.24" ], "nt": "ATGCGAACTATTGCTATTGTCGGAAAGCCAAATGTTGGTAAATCATCATTATTTAATCGTATTTTAATGCGCCGTAAATCAATTGTTGACGATCAGCCAGGAGTAACAAGAGATCGAATATATGATATTGGTAATTGA", "aa": "MRTIAIVGKPNVGKSSLFNRILMRRKSIVDDQPGVTRDRIYDIGN", "aa_hexdigest": "f2b87e50613dbd2af1d693c6f5bc44c1", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00293EF8CC", "query_cov": 0.9555555555555556, "subject_cov": 0.8113207547169812, "identity": 0.674, "score": 65.5, "evalue": 2.67e-13, "valid": false, "product": "GTPase", "uniref50_id": "UniRef50_A0A533SGT9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02421.24", "name": "FeoB_N", "length": 37, "aa_cov": 0.8222222222222222, "hmm_cov": 0.972972972972973, "evalue": 1.5425199106807144e-08, "score": 29.869476318359375, "start": 3, "stop": 39 } ], "seq_stats": { "molecular_weight": 5101.910000000001, "isoelectric_point": 10.903167152404784 }, "id": "GPKLONFDEH_782", "locus": "GCA000006625_00759" }, { "type": "cds", "sequence": "contig_1", "start": 442183, "stop": 442467, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTTTATTAATTCACAAAGATTTTAATATACATTTTTTAGTTATAAATATCGAAAAAAAATATAATTACTCGTTACGAACACAAATTTTAATATATCTTAGTAAAAAGTACTCAAATGAAAATACTTTAATGTTAATTAAATTAATGATTTTTAATGAAAAGATTAACAGTGATTTTAAGCAAATTTTATATGATCTTAATATCGCTCATTTATTTATTGTTTCAGGATTGCACTTGAATTTATTTATTCTAATCATTAATAAGATTTTTTCTAAACGATGA", "aa": "MCLLIHKDFNIHFLVINIEKKYNYSLRTQILIYLSKKYSNENTLMLIKLMIFNEKINSDFKQILYDLNIAHLFIVSGLHLNLFILIINKIFSKR", "aa_hexdigest": "7675bde361b9632d92c8229e549d8365", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11305.474999999986, "isoelectric_point": 9.58988170623779 }, "id": "GPKLONFDEH_783", "locus": "GCA000006625_00760" }, { "type": "cds", "sequence": "contig_1", "start": 442558, "stop": 442884, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATATTTTATCAATTAAATTTTTTCAAAAAATTTCTAAAATGGATAAATTGGCAATTAGCGGAATGTTAATTGGTTGTTTAAGTATTTATAATTTATCGATTTTGAGTTTTATTTTTGCATATTTAGCTTTATTTTGCATTTACCTTATTAATATTTTTATAAAGAACAATTCGGTATTAAAAAATATTTACATAAATTTGTATATTTTATCAATCACTTTTGTCATTTCAATCAATTTAAACCAAAAAATCAATTTTTTAAGTATTGTTTTTGGTTATGTAATGAATTTACCAATTCTTTTTGTATATCAAATACTTTTTTGA", "aa": "MHILSIKFFQKISKMDKLAISGMLIGCLSIYNLSILSFIFAYLALFCIYLINIFIKNNSVLKNIYINLYILSITFVISINLNQKINFLSIVFGYVMNLPILFVYQILF", "aa_hexdigest": "eb7f2dab7e1569cfe694003caaf98452", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12547.236799999982, "isoelectric_point": 9.51774158477783 }, "id": "GPKLONFDEH_784", "locus": "GCA000006625_00761" }, { "type": "cds", "sequence": "contig_1", "start": 442888, "stop": 443031, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTCCTCATTTTGAATTAATTTTAGATTTATTTACTAATACATTAGTAAAATCTATTTATTTTATTAGTGAAATTGATGCGAGTATTCTAATTAAAGCTTACGTAAAAATTGTTACGATTTTTTATACGACTATTTGA", "aa": "MFIPHFELILDLFTNTLVKSIYFISEIDASILIKAYVKIVTIFYTTI", "aa_hexdigest": "0f807bfa75b0d4738f38a744bc43ad46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5499.540899999999, "isoelectric_point": 5.464750862121583 }, "id": "GPKLONFDEH_785", "locus": "GCA000006625_00762" }, { "type": "cds", "sequence": "contig_1", "start": 443158, "stop": 443595, "strand": "+", "frame": 1, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143", "RefSeq:WP_010891757.1", "SO:0001217", "UniParc:UPI000012F557", "UniRef:UniRef100_Q9PQA5", "UniRef:UniRef50_Q9PQA5", "UniRef:UniRef90_Q9PQA5" ], "nt": "ATGTTTATTGGTACTTATAATCATTCAATTGATTCAAAGAATCGTATGTTAGTTCCATCAAAAGTTAAAGCAACATTAGGTGAAGCCATTTTTGTTTATTTATCACTTGGTTTTGATGGAAATATAGATATGAGATTAGAGAGTGAATTTAATCAATTCGTTAACAATATTAATAATTTATTAATTGGTTCAAAAGAAGCGCGAAATCTTACAAGATTAATATTAAGCCAAACTTATAAAATAGAAATCGATAGTGCGTCAAGAATTTTAATACCTCAAAACCTTATTGATAAAGCTAAAATTAAAAAAGATATTTATATTATTGGAACAAATGATCGATATGAAATTTGGGCTAAAGAAGTTTATGATGATTTTAGCTTAAATCAAGAAAGTACGCTAAGTGATTTAGCTGAAAAATTATTAATTAATGGAATTTAA", "aa": "MFIGTYNHSIDSKNRMLVPSKVKATLGEAIFVYLSLGFDGNIDMRLESEFNQFVNNINNLLIGSKEARNLTRLILSQTYKIEIDSASRILIPQNLIDKAKIKKDIYIIGTNDRYEIWAKEVYDDFSLNQESTLSDLAEKLLINGI", "aa_hexdigest": "32b24abd7c28ad5497ebc5810ab6777d", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI000012F557", "ncbi_nrp_id": "WP_010891757.1", "uniref100_id": "UniRef100_Q9PQA5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012F557", "RefSeq:WP_010891757.1", "UniRef:UniRef100_Q9PQA5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQA5", "uniref90_id": "UniRef90_Q9PQA5" }, "psc": { "uniref90_id": "UniRef90_Q9PQA5", "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "uniref50_id": "UniRef50_Q9PQA5", "cog_id": "COG2001", "cog_category": "J", "go_ids": [ "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQA5" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "GPKLONFDEH_786", "locus": "GCA000006625_00763" }, { "type": "cds", "sequence": "contig_1", "start": 443585, "stop": 443953, "strand": "+", "frame": 2, "gene": "mraW", "product": "MraW methylase family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4DTB8" ], "nt": "ATGGAATTTAATCAACACATTACCGTTTTATTAAACGAAACAATCGAATTATTAAATATTAAACCAGATGGCATTTATGTTGATTGTACATTTGGTCGTGGCGGACATTCACAACTCATCTTAAAAAAACTCTCAAAAAAAGGTAAATTAATTTGTTTAGATCAAGATCAAGAAGCAATTAATTTTGCAAATAATTTATTTAAAAATAATACAAATGTAATTGTTATTAAAACAAACTTTAAAAATTTAAAATCAGCATTAAGTGCACATAAAATTTTTTATGTTGATGGCTTTATCTTTGATTTAGGTTTATCAAGTCCACAACTTGATGATCCAAAAAGAGGTTTTAGTTATCATAAGAATGCTTGA", "aa": "MEFNQHITVLLNETIELLNIKPDGIYVDCTFGRGGHSQLILKKLSKKGKLICLDQDQEAINFANNLFKNNTNVIVIKTNFKNLKSALSAHKIFYVDGFIFDLGLSSPQLDDPKRGFSYHKNA", "aa_hexdigest": "c84b2a1e13ca361dba814d2f1a981f61", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4DTB8", "query_cov": 0.9672131147540983, "subject_cov": 0.8082191780821918, "identity": 0.5, "score": 119.0, "evalue": 2.59e-32, "valid": false, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_D4DTB8", "go_ids": [ "GO:0016434" ] }, "pscc": { "uniref50_id": "UniRef50_D4DTB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4DTB8" ], "product": "MraW methylase family" }, "genes": [ "mraW" ], "id": "GPKLONFDEH_787", "locus": "GCA000006625_00764" }, { "type": "cds", "sequence": "contig_1", "start": 443960, "stop": 444511, "strand": "+", "frame": 2, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424", "SO:0001217", "UniRef:UniRef50_Q9PQA4", "UniRef:UniRef90_Q9PQA4" ], "nt": "ATGCGAATGGATCAATCGCAAAACTTAAATGCTCACTATATTGTTAATAATTATTCTTTTGCAAAATTAGTAAGTATTTTTAAACGATATGGAGAAATAAAATATCCAAAAATTATTGCTGATGCAATTGTTAAAGAACGCAGTATTAAAGAGATTAATACAACATTGGAATTAGTTGAAATCATTAAAAAATATTCTCCTAAAAAAAATTTATTTGAAAAAAAACATCCTGCACGTTTATTTTTTCAAGCAATTAGAATAGAAGTTAATGATGAATTAAACATTTTAGAAAAAGCATTTAATGATGCTATTTCAATGTTAAATCCCTTAGGTGTTGTGGCAATTATTAGCTTTCATAGTCTTGAAGATAAAATTGTTAAAAAGGTCTTTAATAATTATGCAAAAATTAAATTACCTAAAGAAGTCCCAATAAATAATTATGTAAATAAATATAGTTTATTGAATCAAAAAATTATGCCAAGCACCCAGGAACTAAATGATAACAATCGTTCACGATCATCTATTTTAAGGGGATTAATTAGAAATTATTAA", "aa": "MRMDQSQNLNAHYIVNNYSFAKLVSIFKRYGEIKYPKIIADAIVKERSIKEINTTLELVEIIKKYSPKKNLFEKKHPARLFFQAIRIEVNDELNILEKAFNDAISMLNPLGVVAIISFHSLEDKIVKKVFNNYAKIKLPKEVPINNYVNKYSLLNQKIMPSTQELNDNNRSRSSILRGLIRNY", "aa_hexdigest": "9a02f6845db6968554e957312940c3c2", "start_type": "ATG", "rbs_motif": null, "genes": [ "rsmH" ], "truncated": "5-prime", "pseudogene": { "start": 443660, "stop": 444511, "observations": { "mutation": [ 443951 ], "stop": [ 443951 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQA4", "query_cov": 1.0, "subject_cov": 0.5941558441558441, "identity": 1.0, "score": 351.0, "evalue": 2.49e-120, "gene_start": 1, "gene_end": 183, "reference_start": 126, "reference_end": 308, "reference_sequence": "MEFNQHITVLLNETIELLNIKPDGIYVDCTFGRGGHSQLILKKLSKKGKLICLDQDQEAINFANNLFKNNTNVIVIKTNFKNLKSALSAHKIFYVDGFIFDLGLSSPQLDDPKRGFSYHKNAWLDMRMDQSQNLNAHYIVNNYSFAKLVSIFKRYGEIKYPKIIADAIVKERSIKEINTTLELVEIIKKYSPKKNLFEKKHPARLFFQAIRIEVNDELNILEKAFNDAISMLNPLGVVAIISFHSLEDKIVKKVFNNYAKIKLPKEVPINNYVNKYSLLNQKIMPSTQELNDNNRSRSSILRGLIRNY" }, "paralog": false, "identity": 0.9964664310954063, "score": 557.0, "evalue": 4.5e-204, "description": "Internal stop codon at 443951. Nonsense mutation around 443951.", "psc": { "uniref90_id": "UniRef90_Q9PQA4", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_Q9PQA4", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQA4", "EC:2.1.1.199", "UniRef:UniRef50_Q9PQA4", "COG:COG0275", "COG:J", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQA4" ], "product": "Ribosomal RNA small subunit methyltransferase H" } }, "id": "GPKLONFDEH_788", "locus": "GCA000006625_00765" }, { "type": "cds", "sequence": "contig_1", "start": 444530, "stop": 445711, "strand": "+", "frame": 2, "gene": null, "product": "SHS2 domain-containing protein", "db_xrefs": [ "RefSeq:WP_006688645.1", "SO:0001217", "UniParc:UPI00000C1C4A", "UniRef:UniRef100_A0AAC9T230", "UniRef:UniRef50_A0AAP9D7J0", "UniRef:UniRef90_A0AAC9T230" ], "nt": "ATGAACAAACCAATGTGTTTGCTTCGTTTAGATAATTATTATTTTACATTGTTGGTATTTATTTATAATGATGTAACTAAAGTTAATGATTTAATTTTTATAAAATCTATTCCAAACAATAATCAAAATAATTATGAATCATCAAATTTTTTGCAAACAGATTTAAAAATCCAACTTAATACATTGATTAACTGTTTTAAAAAAGAATTGCACATTAATTTTATTGATGAAATTATTGTTAGTTATGGTTTTAGTAGCTTTCAAACTACACATACTACTTTTTATAACTATAATGAATTTATGAAATTAAGATCAAATTTTAATAATGAACACAGTGAATTATTAAGTTCAGATCAAAAAAGTTATATAGCAGTTGATTTTGGAATAAGCCACTATTCTAAAGAAAATAGTTATAAGTTGATTTCATATTATATTTTGAAATATGATTATCAATTAATAATTGATTTGTGCCAATCATTTAATTTAAAAATTAAGCAATTTATAGTTTCACAAGCTCATTTAAACGGTTTAAAATTTGATAATGAAGATAATAAAACTTCATTATTATTAGAATTAGAAAATTTTAAAATAAATTTTAGAACTTATAATGAATATAATTTAATTAGTAATGTTATTAGTGAGACAAAAAACCACTTTTGCCTGGCTGATATCATTACTCAAACAGCACAATCATTAAATATTAATCCTAAAAAGATTGAGAGCTACTTTAATGATATTAATTATAATAATAAAAAAGTTATTGAAATTAATATTGATCCTAAAGAGTTGTTTACTTATAGTTTTATTTGTGGTGCGGATATTGAAAATACTTTTTTAAAAATTTTTAAAGAACATTTTGAAAAAAGTATTTTATCTCAATTTGATCATCCATTACTAGATTTTAATCCAAAGAATTTAGCCTTTCTTTTTATTAACACTCCTTTCAATAATTTAAATAAGATATTTTATTATTTTTTTAGTTCATATTTTAAAGAAGTTAAAATTGAAGTTTTTCAAAATAAATTTTTTATTTATTCAAATTGTTATGAATATAGTAATAGTCAAGCCCTAGCTAAGCAAGATTTTTACAATGTACATAACATGAAAAATTTTGATAGCTTTAGCAATGAAAATATCCTAACAAAGATGCATGATCAAACAATAAACATAAAAAATTTTTAA", "aa": "MNKPMCLLRLDNYYFTLLVFIYNDVTKVNDLIFIKSIPNNNQNNYESSNFLQTDLKIQLNTLINCFKKELHINFIDEIIVSYGFSSFQTTHTTFYNYNEFMKLRSNFNNEHSELLSSDQKSYIAVDFGISHYSKENSYKLISYYILKYDYQLIIDLCQSFNLKIKQFIVSQAHLNGLKFDNEDNKTSLLLELENFKINFRTYNEYNLISNVISETKNHFCLADIITQTAQSLNINPKKIESYFNDINYNNKKVIEINIDPKELFTYSFICGADIENTFLKIFKEHFEKSILSQFDHPLLDFNPKNLAFLFINTPFNNLNKIFYYFFSSYFKEVKIEVFQNKFFIYSNCYEYSNSQALAKQDFYNVHNMKNFDSFSNENILTKMHDQTINIKNF", "aa_hexdigest": "9bcdb318c74dce0b1a678f52b97260b0", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00000C1C4A", "ncbi_nrp_id": "WP_006688645.1", "uniref100_id": "UniRef100_A0AAC9T230", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C4A", "RefSeq:WP_006688645.1", "UniRef:UniRef100_A0AAC9T230" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T230", "uniref90_id": "UniRef90_A0AAC9T230" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T230", "uniref50_id": "UniRef50_A0AAP9D7J0" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D7J0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D7J0" ], "product": "SHS2 domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_789", "locus": "GCA000006625_00766" }, { "type": "cds", "sequence": "contig_1", "start": 445807, "stop": 446283, "strand": "+", "frame": 1, "gene": "abiF", "product": "Abi family protein", "db_xrefs": [ "COG:COG4823", "COG:V", "SO:0001217", "UniRef:UniRef50_A0AAC9T0D9", "UniRef:UniRef90_A0AAC9T0D9" ], "nt": "ATGGCGTTAAGTAAACACAGTATTGGTTTGATTAAAAATTTAAAGAATAAAGGATTGACTATTAAAAATAAAGAAAAATTCATTAGTTTCTTGTTTAATTATGGAGTCATAAGAGTTATAGATGGTTATTCATTTTTTTTAAAAAAAGATAATCAATATGTAAAAGGCTTAAAAAGTAAGGATTTAATTAATTTTGTTTTATTAGATCGTAAAATTGCTAATTTAATGGGTGATTGTATTATGCTATTTGAACAACAATTAAATGCTATTGTTGTTCAAAATTTTTTAAAGATCAATAATTTAAATTCAAAATATGTTATAGATATGCGAACAGATCCTTTTATAAAATTTGAATCACCAAAACATTATGAAATATTATCAGAAAATATTTACACATATGCTAATTCTTCTAATTTGTTAAGTCAATTTAATACTAACGCACAAGAAATTCCTTTAATTAATTTAGCATTATCATGA", "aa": "MALSKHSIGLIKNLKNKGLTIKNKEKFISFLFNYGVIRVIDGYSFFLKKDNQYVKGLKSKDLINFVLLDRKIANLMGDCIMLFEQQLNAIVVQNFLKINNLNSKYVIDMRTDPFIKFESPKHYEILSENIYTYANSSNLLSQFNTNAQEIPLINLALS", "aa_hexdigest": "90aceaedd8dce4060193e0fae39685e3", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [ "abiF" ], "truncated": "3-prime", "pseudogene": { "start": 445807, "stop": 446586, "observations": { "mutation": [ 446281 ], "stop": [ 446281 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0D9", "query_cov": 1.0, "subject_cov": 0.5429553264604811, "identity": 1.0, "score": 306.0, "evalue": 3.17e-103, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MALSKHSIGLIKNLKNKGLTIKNKEKFISFLFNYGVIRVIDGYSFFLKKDNQYVKGLKSKDLINFVLLDRKIANLMGDCIMLFEQQLNAIVVQNFLKINNLNSKYVIDMRTDPFIKFESPKHYEILSENIYTYANSSNLLSQFNTNAQEIPLINLALSWTLNTTIGFYRAVDNNVQISVLEYFQLNHITPLEFIDILHIIKHFRNSISHNDMVITNSFNDNVGAFAKSLKLKSIKKVVGFLDVAAFLTAFTNDKFHVLNGNLHIKILQAIKKAKFKGVVLEHINNLLGVED" }, "paralog": false, "identity": 0.9961389961389961, "score": 507.0, "evalue": 5.68e-185, "description": "Internal stop codon at 446281. Nonsense mutation around 446281.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0D9", "gene": "AbiF", "product": "Abi family protein", "uniref50_id": "UniRef50_A0AAC9T0D9", "cog_id": "COG4823", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0D9", "UniRef:UniRef50_A0AAC9T0D9", "COG:COG4823", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0D9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0D9" ], "product": "Abi family protein" } }, "id": "GPKLONFDEH_790", "locus": "GCA000006625_00767" }, { "type": "cds", "sequence": "contig_1", "start": 446440, "stop": 446682, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATTACTAATAGTTTCAATGATAACGTAGGTGCATTTGCTAAATCATTAAAATTAAAAAGTATTAAAAAAGTAGTGGGATTTTTAGATGTTGCGGCTTTTTTAACGGCGTTTACAAACGATAAATTTCATGTTTTAAATGGTAATTTACACATCAAAATTTTACAAGCAATTAAAAAAGCAAAATTCAAAGGCGTTGTTTTAGAACATATTAATAATTTATTAGGAGTTGAAGATTAA", "aa": "MVITNSFNDNVGAFAKSLKLKSIKKVVGFLDVAAFLTAFTNDKFHVLNGNLHIKILQAIKKAKFKGVVLEHINNLLGVED", "aa_hexdigest": "5aef93447298862e966d05e1a95e965a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8810.314699999999, "isoelectric_point": 9.757370567321775 }, "id": "GPKLONFDEH_791", "locus": "GCA000006625_00768" }, { "type": "cds", "sequence": "contig_1", "start": 446687, "stop": 447133, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGATCAAGGAGTAAATAACAAGGCAATTAAGCAAAAAGTCTTAAATTTACTATCAACTGATACGCGTGATAGTTGTATAAGAACACGCTTAACAAAATACCATTTAGATATTAGTCACACTTTTAATAAATATGAACTTCAAGATTTTATTGAAGGTAAAATTAACATTCTTAAAATTAGAAATTTTATTAACATCAATACGTTTGAAAATGATGTTAAAAATATAAATTCAATGGATGAATTGATTGATTATTTACAGGATTTCAATTTAAAACTAAAAGATCTTTTATCGACACGTAAAATTAATGAATGGAGCCAAGACTTTGTATTTTATAAAAATGAACGAATTGCTGATATGATTAAAGCGATTGAAAGTCAACCAAAAAAATTTGAATATTTAAGTCGTGAAGCACGCAATATTTATGCGCAAACAGGTGTTTGA", "aa": "MQDQGVNNKAIKQKVLNLLSTDTRDSCIRTRLTKYHLDISHTFNKYELQDFIEGKINILKIRNFININTFENDVKNINSMDELIDYLQDFNLKLKDLLSTRKINEWSQDFVFYKNERIADMIKAIESQPKKFEYLSREARNIYAQTGV", "aa_hexdigest": "bef0e5f63cb1ac873d906a4b20ba3128", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17602.872999999985, "isoelectric_point": 8.572828483581542 }, "id": "GPKLONFDEH_792", "locus": "GCA000006625_00769" }, { "type": "cds", "sequence": "contig_1", "start": 447158, "stop": 448150, "strand": "+", "frame": 2, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00242D11F6", "UniRef:UniRef90_UPI00242D11F6" ], "nt": "TTGATTGGTACAACACCTAAAGCTTCAAATATTAACGCGCCCTTAATTTTTGTTGATGTTGAGATTAAAAAATATAATGGTGAAATTTTTTTAAAACGAAAAAATACTAATTTTATTGTTAACGAAAAAATACTATCTTTTTTAAAAAATGATTATAAAAATAATATCGTTTCTTTAAATGAAATTAAAAACTTTAGTTATTTAGAAATTCAAACTCTAATTAAAAAAATAATACTAAAAGATCATTTAGAATCGATTATTAATAATAATGAATTTCTAAACTTAAAACAAAATGAAATTAATGAGCAATTTAATAACTTTTTTTTATATGATGCATATGTTTTAGGAATTTTTGAGCCCCATGGAGGAGCGATTAAAAATGACTTAGAAGTGATTATTAGTAATAATATTGATCCTTTTGACGAAGAAATAAATAATGTTAATCAACAAATTTATTATAAAAACTATCATTTTTTAGCAGAAGAAAATAAGCTTCCTTTAATCCAAATTAATCGTCCTTTAAATATTTTTCAAAAGTATGCTATTCATTCAGGATTAGTTAAAAATACTTTAATATATGGACCGCCTGGAACGGGAAAATCAGAGGTGATTGCAAGTATTATTGCAAACGTAATTAATGATTTAAAATCGATTTTAATTATTTCGGAGAAAAAAGCTGCTTTAGATGTATTACATGAAAGATTAGCTAAAATTAATTTTTTCACACTTTTTATTTATGATACAAATAATGATGATCAATTTTATAATAACATTTTTAATTTAATTGAAAAAATAGGAGGATTGCGTTTTATTGAGAATCAAGATTTAAACCAATTCAATGATTTTAAATATAAAAATAATATTATTTCAAACATTTATTATAAAAAGATTCATGAATTAAAAAAAGAAATTATTAATATTACATCTTTAATTAAAAATTTACATAATGAAAAAGATAATAATGATAATAATTTTGCTAATTATTTGTATTGA", "aa": "MIGTTPKASNINAPLIFVDVEIKKYNGEIFLKRKNTNFIVNEKILSFLKNDYKNNIVSLNEIKNFSYLEIQTLIKKIILKDHLESIINNNEFLNLKQNEINEQFNNFFLYDAYVLGIFEPHGGAIKNDLEVIISNNIDPFDEEINNVNQQIYYKNYHFLAEENKLPLIQINRPLNIFQKYAIHSGLVKNTLIYGPPGTGKSEVIASIIANVINDLKSILIISEKKAALDVLHERLAKINFFTLFIYDTNNDDQFYNNIFNLIEKIGGLRFIENQDLNQFNDFKYKNNIISNIYYKKIHELKKEIINITSLIKNLHNEKDNNDNNFANYLY", "aa_hexdigest": "fc39b2fd1ba2499628a7d157ba950fcf", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 446858, "stop": 448084, "observations": { "mutation": [ 447131 ], "stop": [ 447131 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00242D11F6", "query_cov": 0.9333333333333333, "subject_cov": 0.6623655913978495, "identity": 0.99, "score": 593.0, "evalue": 3.23e-211, "gene_start": 1, "gene_end": 308, "reference_start": 158, "reference_end": 465, "reference_sequence": "MQDQGVNNKAIKQKVLNLLSTDTRDSCIRTRLTKYHLDISHTFNKYELQDFIEGKINILKIRNFININTFENDVKNINSMDELIDYLQDFNLKLKDLLSTRKINEWSQDFVFYKNERIADMIKAIESQPKKFEYLSREARNIYAQTGVWPLYIAKNFLIGTTPKASNINAPLIFVDVEIKKYNGEIFLKRKNTNFIVNEKILSFLKNDYKNNIVSLNEIKNFSYLEIQTLIKKIILKDHLESIINNNEFLNLKQNEINEQFNNFFLYDAYVLGIFEPHGGAIKNDLEVIISNNIDPFDEEINNVNQQIYYKNYHFLAEENKLPLIQINRPLNIFQKYAIHSGLVKNTLIYGPPGTGKSEVIASIIANVINDLKSILIISEKKAALDVLHERLAKINFFTLFIYDTNNDDQFYNNIFNLIEKIGGLRFIENQDLNQFNDFKYKNNIISNIYYKRIHELKKEIIDIT" }, "paralog": false, "identity": 0.9926470588235294, "score": 804.0, "evalue": 8.56e-297, "description": "Internal stop codon at 447131. Nonsense mutation around 447131.", "psc": { "uniref90_id": "UniRef90_UPI00242D11F6", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI00242D11F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00242D11F6", "UniRef:UniRef50_UPI00242D11F6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00242D11F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00242D11F6" ], "product": "DUF4011 domain-containing protein" } }, "id": "GPKLONFDEH_793", "locus": "GCA000006625_00770" }, { "type": "cds", "sequence": "contig_1", "start": 448199, "stop": 448645, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCAATGAAATTAATAATTTAATTGAGAATTATTCTTATAATTTTGATGAAATTATTACACACGTTAAAGCTAGTTATGATTTTTTTAAAAAATATAATTATGAATTTTCTAAAAATAATTTTTTAAAATTTAAAGAAGAAAGCGAACAATTAAATAAATTACAAATTGATTTTAATTTAATTACAAAATCTTTATATCAATCACAAACTTTTCTACCACGTATTAAGTTGTTAGAAAGCTTTTTAGTTAAAAATAATATGACTTTAACAAGTCTTATATATGATGATGTTAACTTGTTAAGTAATACATATGTTGAAGCTAATGAGTTTTATACAAACTTTAATCATCTTTTAAAAGATGATTTTAAAAAATTTATTGAAGATAATATAAAAAGTCATTCATTGTTTTTTAGTCATTTAAATACTTTAAAAAATAATGAATGA", "aa": "MINEINNLIENYSYNFDEIITHVKASYDFFKKYNYEFSKNNFLKFKEESEQLNKLQIDFNLITKSLYQSQTFLPRIKLLESFLVKNNMTLTSLIYDDVNLLSNTYVEANEFYTNFNHLLKDDFKKFIEDNIKSHSLFFSHLNTLKNNE", "aa_hexdigest": "0b028ebe6cbbd9864b272b9a0a43f5ce", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17846.920899999997, "isoelectric_point": 5.582180213928221 }, "id": "GPKLONFDEH_794", "locus": "GCA000006625_00771" }, { "type": "cds", "sequence": "contig_1", "start": 449069, "stop": 449296, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTAAAAAATTAAGAGATGCAAATATTCAATTTTCTAAAAATGCTGATGAAATTATTTACAATAACTATTTAGATTATTTGCAAATTAAATTAGTTACTGCTCCTAAACAAATCCAAACAAAAATTAAAGAAATTGCGCAAATTTGTCGTTTAGCAAAAAAACCTAATATTGTGAAATTTATTTTAAAATATTATGAAATTTTACGTTTTCTTTTCCCTATTTGA", "aa": "MCKKLRDANIQFSKNADEIIYNNYLDYLQIKLVTAPKQIQTKIKEIAQICRLAKKPNIVKFILKYYEILRFLFPI", "aa_hexdigest": "d7022c60d316f8b67be0c9762e3ad73f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8979.708099999996, "isoelectric_point": 9.697543907165528 }, "id": "GPKLONFDEH_795", "locus": "GCA000006625_00772" }, { "type": "cds", "sequence": "contig_1", "start": 449324, "stop": 450400, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "ATGTTACCATTAATAAGTAAGAGTTTTGATTATGGAATTTTTGATGAGGCTTCTCAGATGTTTTTAGAACGTTCTTATCCTTCAATCTATCGTTGTAATATTAATATTGTTGCAGGCGATGATAAACAATTATGCCCTACTAATTTTTTTATTAATCGTCAAGAAAATGAAAACGATGAGTTTGAATTAGCAGATGTAGATGTTGCAGAAAGTTTATTAGATCGTGCTAAAACTGCTCTATGGTCAGAATATTTATTAAAAAACCACTATCGTTCTAATCGAGAAGATTTAATTAGTTTTAGTAACGATAATATTTATAATGGCGATTTACATTTTGCATCTAAAAATGGGTTATTTGATCCAGGAATGGAAATTATAGATGTTGAAGGTGTATTTGAAAATGAAAATGTTATTGAAGCACAAAAAATTATGGAGGTTTTAAAAGAACGAGCAGCCAATTATCAAAAAATTATTGTTATAACTTTTAATATCAAACAAAGTGAATTAATTGAAAATTTAATACTACAAACTTTTAGTTTTAATGATGTTGTTTATCAACGTTTTGAGAATGATGAAATTATTGTTACTAATTTAGAAAACGTTCAAGGTAATGAAGCTGATTTAGTAATTTTATCGGTTACTTATGCCAAAAATAAAGAAGGAATTTTACGCAATAATTATGGCCCATTAATGAAAAAAGGTGGTGCTAATCGGTTAAATGTAGCGATTACAAGAGCAGCAGATAAAATGATTGTTGTTAAATCAATTAAAGCTGTGGATATGATGGTTAATATTAATAATGATAATATTAGGACTTTTAAAAATTTTATTGGTTATTGTGATGAAATTGTTCATTTGACTAATCAAAATAAATTTAATGATTGTAGTCATTTTGATGAAGAATTTTTAACAAGTTTAGAAAAAACCATATTGAAAATTTTAAAAGATTATAAAAAAACAAATCAACAAATTTTATATAAACTAAAAATTGGTAATTTTAAAATCACTTTTGCTTTATATAATGAAGAAAAACAAAAAATCGATTTACTTATTTTTATAGAAGAGTTTAATGAATGA", "aa": "MLPLISKSFDYGIFDEASQMFLERSYPSIYRCNINIVAGDDKQLCPTNFFINRQENENDEFELADVDVAESLLDRAKTALWSEYLLKNHYRSNREDLISFSNDNIYNGDLHFASKNGLFDPGMEIIDVEGVFENENVIEAQKIMEVLKERAANYQKIIVITFNIKQSELIENLILQTFSFNDVVYQRFENDEIIVTNLENVQGNEADLVILSVTYAKNKEGILRNNYGPLMKKGGANRLNVAITRAADKMIVVKSIKAVDMMVNINNDNIRTFKNFIGYCDEIVHLTNQNKFNDCSHFDEEFLTSLEKTILKILKDYKKTNQQILYKLKIGNFKITFALYNEEKQKIDLLIFIEEFNE", "aa_hexdigest": "b317551be91cddb801aa89ff48150ccc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 209, "aa_cov": 0.5167597765363129, "hmm_cov": 0.9090909090909091, "evalue": 3.151826815924361e-18, "score": 61.598480224609375, "start": 69, "stop": 253 } ], "seq_stats": { "molecular_weight": 41561.78549999999, "isoelectric_point": 4.874762153625488 }, "id": "GPKLONFDEH_796", "locus": "GCA000006625_00773" }, { "type": "cds", "sequence": "contig_1", "start": 450446, "stop": 450541, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCGTGGTTATAAAACAATCGTTATTAATGATTATGAAATTTGTTTAAACTATAATCAAGTTAAAAATAATATTATTAAATTAATTGATTAA", "aa": "MDRGYKTIVINDYEICLNYNQVKNNIIKLID", "aa_hexdigest": "3c66fbb2fa8074782631b0e3ef4e13c6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3717.2728000000016, "isoelectric_point": 6.006028556823729 }, "id": "GPKLONFDEH_797", "locus": "GCA000006625_00774" }, { "type": "cds", "sequence": "contig_1", "start": 450602, "stop": 450916, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTAAAGTCATTTGAATTTTTAAAAGGTGATGCAATTGTTGATAACCAAATAAAAAAAGCTATTAATACTAGTTTGATTAAAGCATCATTTTCATGTGCAATTATGTGTATATTAGTTCTTATTTTTGGTTTTTTAATGCGAAAATATTTATTTTATTTTTCAGATGCATCTAGTTTAATTATTAGCTCTCGTTTGATTTTTCAAATAATTACAATCATTGTTGCTACTTTATCAATTGTCTATTTTATTGTTCATTTAATCATTTACTTTAGACAAAGAAATAATTTTTTAGCTCCTAAATTTGCATGA", "aa": "MNLKSFEFLKGDAIVDNQIKKAINTSLIKASFSCAIMCILVLIFGFLMRKYLFYFSDASSLIISSRLIFQIITIIVATLSIVYFIVHLIIYFRQRNNFLAPKFA", "aa_hexdigest": "0854ca84e862b1aaec9a068c48696db5", "start_type": "TTG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11969.341199999983, "isoelectric_point": 9.799210548400879 }, "id": "GPKLONFDEH_798", "locus": "GCA000006625_00775" }, { "type": "cds", "sequence": "contig_1", "start": 450950, "stop": 451228, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTTTTATGATTAGTTATGTAGCGGCGATTGTAGGATTAGATTTGACGTTAATAGCCATTGGAATTAGTTTATCAATCATTGTCGCAATGGGAATACTAGGTTTTATTATGAATCATAAAATTGCTTTTAAATTAGGCATTGCTAATATCGTTTTATTTACTATTGCTACAATTGGTTCAATTATTTTATTGATTGTATTTTTTACAACGAGATATAACCATACTGGTTTTAACAAAGGTGTTATTATTGCTGACATTGTATTAAGTATTATTTGA", "aa": "MCFMISYVAAIVGLDLTLIAIGISLSIIVAMGILGFIMNHKIAFKLGIANIVLFTIATIGSIILLIVFFTTRYNHTGFNKGVIIADIVLSII", "aa_hexdigest": "c5f9e49a04e11f56c3745eee6042c886", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9886.075299999986, "isoelectric_point": 9.035453605651856 }, "id": "GPKLONFDEH_799", "locus": "GCA000006625_00776" }, { "type": "cds", "sequence": "contig_1", "start": 451524, "stop": 452150, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF13191.12", "PFAM:PF07728.20", "PFAM:PF05621.17" ], "nt": "ATGGAATTTAAACAAAAAAACTCAAACAATCCATTAGAGGAATTTGGACGTAATTTAAATAAGGAAATTTTAGATAATAAAATTGACCCCATTATCGGTCGAGATGAAGAAATTAGAAGAACCATTGAAATTCTAAGTCGTAAAACTAAGAATAATCCTGTCTTAATTGGTGAACCTGGTGTTGGTAAAACAGCAATTGTTGAGGGGCTTGCATACCGAATTGTTCATAAAGATGTTCCTTCAAACTTATTAGATAAAACAATTATTGAATTATCATTATCATCTTTAATTGCAGGCGCTAGTTATCAAGGCCAGTTTGAGGAACGAATTAATAGTATTTTAAAAGAAGTCAAAAAAGCAGATGGTAATATCATTTTGTTTATTGATGAAATACATCAAATTGTTGGTATGGGTAGAAATCAAGGTTCAAATATGGATGTTGCTAATATTTTAAAACCAATGATGGCAAGAGGAGAAATCAAATTAATTGGCGCCACAACGCTTGATGAGTATCGTTTATATATTGAAAAAGATCAGGCATTAGAACGTCGTTTTACAAAAGTTTTAGTTAACAAACCAACAATTCAAGAAACTTTAACAATAATGCGAGGTTTAAAGCCACGGTGA", "aa": "MEFKQKNSNNPLEEFGRNLNKEILDNKIDPIIGRDEEIRRTIEILSRKTKNNPVLIGEPGVGKTAIVEGLAYRIVHKDVPSNLLDKTIIELSLSSLIAGASYQGQFEERINSILKEVKKADGNIILFIDEIHQIVGMGRNQGSNMDVANILKPMMARGEIKLIGATTLDEYRLYIEKDQALERRFTKVLVNKPTIQETLTIMRGLKPR", "aa_hexdigest": "f0b7490c62b8d24956fbc219301f0976", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_A0A2X1PP87", "query_cov": 0.9663461538461539, "subject_cov": 0.8375, "identity": 0.5870000000000001, "score": 243.0, "evalue": 1.76e-78, "valid": false, "product": "Protein disaggregation chaperone", "uniref50_id": "UniRef50_A0A453E693" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 141, "aa_cov": 0.6394230769230769, "hmm_cov": 0.8723404255319149, "evalue": 3.0762544459352004e-18, "score": 62.11042022705078, "start": 54, "stop": 186 }, { "id": "PF13191.12", "name": "AAA_16", "length": 51, "aa_cov": 0.23076923076923078, "hmm_cov": 1.0, "evalue": 2.94372701235383e-09, "score": 33.20856857299805, "start": 31, "stop": 78 }, { "id": "PF07728.20", "name": "AAA_5", "length": 81, "aa_cov": 0.38461538461538464, "hmm_cov": 0.9259259259259259, "evalue": 1.9577926483864373e-06, "score": 23.49312400817871, "start": 54, "stop": 133 }, { "id": "PF05621.17", "name": "TniB", "length": 152, "aa_cov": 0.6634615384615384, "hmm_cov": 0.9671052631578947, "evalue": 5.5424054678138e-09, "score": 31.295740127563477, "start": 53, "stop": 190 } ], "seq_stats": { "molecular_weight": 23551.060400000006, "isoelectric_point": 8.99960918426514 }, "id": "GPKLONFDEH_800", "locus": "GCA000006625_00777" }, { "type": "cds", "sequence": "contig_1", "start": 452235, "stop": 453521, "strand": "+", "frame": 3, "gene": "clpA", "product": "ATP-dependent serine proteinase-heat shock protein", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_V5RI22", "UniRef:UniRef90_Q9PQ99" ], "nt": "TTGCCAGATAAAGCTATTGATTTAATTGATGAAGCAGCTGCAAAGATTAAAACGCAAATTAATTCACAACCAATATATTTAGATGAAATTAAACGTGATTTACAACATTTACAAACTGAAAAAGCCGCTTTAGAACCAGAAAAAGATGAAAAATCAATTAAACGTTTAAATGAAATTTTAGAAAAAATTAAATTAAAACAAGATGAATTTAACAATTTAAATGATTTGTATTTAAAAGAAAAAAAGCAAATTGACGATTTAAAAAATTTACGGCAAAAAATTGAAAGAATTCAACATGATATTGAATTTTATCAATCTGAGGGTAAGTATGAAAAAGCATCAAGATTACTTTATTCAGATTTACCATTATTTGAAAAACAACGAGAAGAATTAGAAAATAAAATTAATGAGAATAATAATCAAAAAATGATTATTGATTCATTAACAGAGAATGAAATTGCTGATGTTATTGCGCGTGCTACAGGAATTCCATTAAGTCATTTATTAGCTGATGAAAAAACAAAATTATTATCGCTAAACAAACGAATTGCTAGTCATGTTATTGGTCAAGATGAAGCAGTTAAATTAATAAGTGATGCGGTAATCCGTGGTCGTGCAGGTATTAATAATCCTAACCAACCTATTGGTTCTTTCTTATTTTTAGGACCTACGGGCGTTGGTAAAACAGAATTAGCAAAAACTTTAGCAAAAGAATTGTTCAATTCAGAAAAAGCCTTAATTAGATTTGATATGAGTGAATATATGGAAAAACATAGTGTATCAAAATTAGTTGGTGCTCCACCTGGTTATATTGGTTATGAAAATGCTGGAGTTTTAACTGAATCTGTTAAACGCAAACCATATAGTATTTTGTTATTTGATGAAATTGAAAAAGCACACCCTGATATTTTAAATATTTTATTACAAATATTGGATGAAGGTAGCATTAAGGATGCTAAAAATAATGAAATTAATTTTAAAAATACAATTATTATTATGACATCTAATATTGGAGCAGAAGCTTTATTAGAAAATAATAAAACAAAGGCATTACTTGATTTACAAAAAACTTTTAAACCAGAAATTTTAAACCGAATTTCTGAAATTATTTTCTTTAATAAGTTATCAAAAGAAATCATTTTTAAGATTAGTGAAAATTTACTTAAAGAATTAGAAAATTTATTAGCTAAGCAAGATTATTTAATTAAATTTGATGATAATATTGCTCAAATCATGGTTGAAGATGCTTATAGTTCAAATTATGGTGCAAGACCTTTAAAACGATGA", "aa": "MPDKAIDLIDEAAAKIKTQINSQPIYLDEIKRDLQHLQTEKAALEPEKDEKSIKRLNEILEKIKLKQDEFNNLNDLYLKEKKQIDDLKNLRQKIERIQHDIEFYQSEGKYEKASRLLYSDLPLFEKQREELENKINENNNQKMIIDSLTENEIADVIARATGIPLSHLLADEKTKLLSLNKRIASHVIGQDEAVKLISDAVIRGRAGINNPNQPIGSFLFLGPTGVGKTELAKTLAKELFNSEKALIRFDMSEYMEKHSVSKLVGAPPGYIGYENAGVLTESVKRKPYSILLFDEIEKAHPDILNILLQILDEGSIKDAKNNEINFKNTIIIMTSNIGAEALLENNKTKALLDLQKTFKPEILNRISEIIFFNKLSKEIIFKISENLLKELENLLAKQDYLIKFDDNIAQIMVEDAYSSNYGARPLKR", "aa_hexdigest": "d16e9756dbebe911d55f71031d542c3f", "start_type": "TTG", "rbs_motif": null, "genes": [ "clpA" ], "truncated": "both", "pseudogene": { "start": 451935, "stop": 453638, "observations": { "mutation": [ 452148, 453519 ], "stop": [ 452148, 453519 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ99", "query_cov": 1.0, "subject_cov": 0.6079545454545454, "identity": 0.897, "score": 725.0, "evalue": 7.58e-258, "gene_start": 1, "gene_end": 428, "reference_start": 238, "reference_end": 665, "reference_sequence": "MEFKQKNTSNPLEEFGRNLNQEILDNKIDPIIGRDEEIRRTIEILSRKTKNNPVLIGEPGVGKTAIVEGLAYRIVHKDVPSNLLDKTIIELSLSSLIAGASYQGQFEERINAILKEVKKSNGNIILFIDEIHQIVGMGRNQGSNMDVANILKPMMARGEIKLIGATTLDEYRLYIEKDQALERRFTKVLVNKPTIQETLTIMRGLKPRWEAFHGIKIHDNALIAAVNLSERYINDRNLPDKAIDLIDEAAAKIKTQINSQPIYLDEIKRDLQHLQTEKAALESEKDEKSIKRLNDILEKIKLKQDEFNNLNDIYLKEKKQIDDLKNLRQKIERIQHDIEFYQSEGKYEKASRLLYSDLPALEKQRGELENKINENSSQKMIVDSLTENEIADVIARATGIPLNHLLADEKTKLLSLNKRIAAHVIGQDEAVKLISDAVIRGRAGINNPNQPIGSFLFLGPTGVGKTELAKTLAKELFDSQKALIRFDMSEYMEKHSVSKLVGAPPGYVGYENAGLLTESVKRKPYSILLFDEIEKAHPDVLNILLQILDEGSVKDAKNNEINFKNTIIIMTSNVGAEALLENNKTNALMELQKTFKPEILNRISEIIFFNKLSKEVIFKISENLLKELSDLLAKQDYSIKFNDNIAKIMVDEAYSSNYGARPLKRWITKHLENEIAKLIIENRITKNRNYEINYDREADQVLIK" }, "paralog": false, "identity": 0.9153439153439153, "score": 1006.0, "evalue": 0.0, "description": "Internal stop codon at 452148, 453519. Nonsense mutation around 452148, 453519.", "psc": { "uniref90_id": "UniRef90_Q9PQ99", "gene": "ClpA", "product": "ATP-dependent serine proteinase-heat shock protein", "uniref50_id": "UniRef50_V5RI22", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ99", "UniRef:UniRef50_V5RI22", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_V5RI22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V5RI22" ], "product": "ATP-dependent Clp protease, ATPase subunit ClpB" } }, "id": "GPKLONFDEH_801", "locus": "GCA000006625_00778" }, { "type": "cds", "sequence": "contig_1", "start": 453846, "stop": 454148, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTACTTTTTAAAAAAAGCACATATTTTTTAGTAGGAATTAGCTTATTTGTTAATTTAATTTTTATAATTATAAATGGTTTAAATATTGCTAATAATATAAAAACGTATTTGATTTTATTTTATCTTTTTACTAGTATTAATTCACTTTTAACAATAGTCTTTAGCACTATTAAAGCTATTAATTTATTCAAAGATTTAAAGGATGAAGGAATTGAAATTTTAGTCTTTTCAAAAAGTATTTCACGTAAAAACATTATTTTTACTAAAATAGGTTTTTTAATATTAAATATTGTTTTTTGA", "aa": "MVLFKKSTYFLVGISLFVNLIFIIINGLNIANNIKTYLILFYLFTSINSLLTIVFSTIKAINLFKDLKDEGIEILVFSKSISRKNIIFTKIGFLILNIVF", "aa_hexdigest": "0adbe596c64db1ad88c5a42c1bee9979", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11458.783899999984, "isoelectric_point": 9.853041648864746 }, "id": "GPKLONFDEH_802", "locus": "GCA000006625_00779" }, { "type": "cds", "sequence": "contig_1", "start": 454317, "stop": 454613, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTCCAATTAGTTGTTTTCTCCCCTTTTTAATTGCTGGTGGTGTTGCTAATTTGTATTCAACATCTAAAATTAATCAAGTAGCCAAATACATGAATATTAATTATGATAAATATGATAGTAATACAATTTTAGATGTAGAAAAATTTTATTTAAATAATCATAATGATGAAGTTTATTTAATCACTAAAAATTTAAATAATCCACAATTTAGTAAACGTCAGAATTATTTTTTGAAAGCAGCGTTTGATCACGCAAAAAATGCATCGACATTTTTTCATGTCTTATCTTGA", "aa": "MIIPISCFLPFLIAGGVANLYSTSKINQVAKYMNINYDKYDSNTILDVEKFYLNNHNDEVYLITKNLNNPQFSKRQNYFLKAAFDHAKNASTFFHVLS", "aa_hexdigest": "abe39ac1ebc38427526e3fea5375d7d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11333.805799999987, "isoelectric_point": 8.688291358947755 }, "id": "GPKLONFDEH_803", "locus": "GCA000006625_00780" }, { "type": "cds", "sequence": "contig_1", "start": 455367, "stop": 455501, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGTAGTTAATAACAAAACTTTATATCGTTACCAACTACAAAAAAGTAATAATTATGTTTTTCAAACAGCAAAAGAAGTTTATTCTATTAAACGTGCTGATCAAATTGTTAATAAAAATTATTATTGA", "aa": "MKKVVNNKTLYRYQLQKSNNYVFQTAKEVYSIKRADQIVNKNYY", "aa_hexdigest": "c858ba5c6254e0a6b1155aa46a55a905", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5393.139200000002, "isoelectric_point": 9.93091945648193 }, "id": "GPKLONFDEH_804", "locus": "GCA000006625_00781" }, { "type": "cds", "sequence": "contig_1", "start": 455606, "stop": 455779, "strand": "-", "frame": 3, "gene": "acpS", "product": "holo-ACP synthase", "db_xrefs": [ "COG:COG0736", "COG:I", "EC:2.7.8.7", "GO:0000287", "GO:0005737", "GO:0006633", "GO:0008897", "SO:0001217", "UniRef:UniRef50_Q9PQ97", "UniRef:UniRef90_Q9PQ97" ], "nt": "ATGAAAGCTTTAAAATTAAAATATGGATATGGAGATATTTTAATTCTGAAAACAGAGAATCAACGCCAAGTTTATCTTAATAAAATTTTAATAAAAGAGTTAGAACTTTCAATTTCATACACAGAAACTTATATAGTTGCTTCTGTAGTAGGTTTAATTAATAATATGAACTAA", "aa": "MKALKLKYGYGDILILKTENQRQVYLNKILIKELELSISYTETYIVASVVGLINNMN", "aa_hexdigest": "721a45fb5f9ccbeabf0f97529595a1fc", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "acpS" ], "truncated": "3-prime", "pseudogene": { "start": 455606, "stop": 455953, "observations": { "mutation": [ 455920 ], "stop": [ 455920 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ97", "query_cov": 1.0, "subject_cov": 0.4956521739130435, "identity": 1.0, "score": 108.0, "evalue": 1.4e-29, "gene_start": 1, "gene_end": 57, "reference_start": 59, "reference_end": 115, "reference_sequence": "MKLVHGIDIIEWNREELNNPLFAKRILIDNELEYYFQLNSSREKKRYLASVFACKEAVMKALKLKYGYGDILILKTENQRQVYLNKILIKELELSISYTETYIVASVVGLINNMN" }, "paralog": false, "identity": 0.991304347826087, "score": 221.0, "evalue": 1.39e-76, "description": "Internal stop codon at 455920. Nonsense mutation around 455920.", "psc": { "uniref90_id": "UniRef90_Q9PQ97", "gene": "acpS", "product": "holo-ACP synthase", "ec_ids": [ "2.7.8.7" ], "uniref50_id": "UniRef50_Q9PQ97", "cog_id": "COG0736", "cog_category": "I", "go_ids": [ "GO:0005737", "GO:0008897", "GO:0000287", "GO:0006633" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ97", "EC:2.7.8.7", "UniRef:UniRef50_Q9PQ97", "COG:COG0736", "COG:I", "GO:0005737", "GO:0008897", "GO:0000287", "GO:0006633" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ97" ], "product": "Holo-[acyl-carrier-protein] synthase" } }, "id": "GPKLONFDEH_805", "locus": "GCA000006625_00782" }, { "type": "cds", "sequence": "contig_1", "start": 455982, "stop": 456257, "strand": "-", "frame": 2, "gene": null, "product": "DivIVA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2D9", "UniRef:UniRef90_A0AAC9T2D9" ], "nt": "ATGGTAAATGATAATAAAATTAATGAGTATAAAAATAAAAAATTTGTTGCTAACAAAGTTGGTTACGATCCTGAAAGTGTAGATGTTTTTTTAGACGAATTAATTAATGAATTAGAAATATTTATAAATGAACATAAAAATGCAATATTAAAAATAGAAGAACTTAATGAATATAAAAGAAAATTCGAAAAAGAACGTGAATACACAACATCACTAAATAATTTTATTGATCTTTTAGAAAAAGTTGTTGATAAAAAAAGCACTTTTGATGTTTGA", "aa": "MVNDNKINEYKNKKFVANKVGYDPESVDVFLDELINELEIFINEHKNAILKIEELNEYKRKFEKEREYTTSLNNFIDLLEKVVDKKSTFDV", "aa_hexdigest": "2d237ba36cc1ee24ababcde319dde9e5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0AAC9T2D9", "query_cov": 1.0, "subject_cov": 0.9191919191919192, "identity": 1.0, "score": 174.0, "evalue": 3.06e-55, "valid": true, "product": "DivIVA domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T2D9" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2D9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2D9" ], "product": "DivIVA domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_806", "locus": "GCA000006625_00783" }, { "type": "cds", "sequence": "contig_1", "start": 456308, "stop": 456508, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3G4", "UniRef:UniRef50_A0AAP9ACM2", "UniRef:UniRef90_A0AAC9T3G4" ], "nt": "ATGTTATTTGGGACAATTATTGCTAATACAGCTATTTCTATGATTGGAGGCATAACAAATATTGTAATGGCTAGTTCATCACAACAAAACCACGATTCACAAGATATAGATTCGATTAAAATAGAAACAATTAATGTAGATATGAGTACAATAGTTGGTCCTAAAATTCCCTATATGAATATTTATTATTTAAATTCTTAA", "aa": "MLFGTIIANTAISMIGGITNIVMASSSQQNHDSQDIDSIKIETINVDMSTIVGPKIPYMNIYYLNS", "aa_hexdigest": "312a6d05e095fe282c6a98739d223e4f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0AAP9ACM2", "query_cov": 1.0, "subject_cov": 0.8918918918918919, "identity": 0.636, "score": 75.9, "evalue": 8.24e-17, "valid": false, "uniref50_id": "UniRef50_A0AAP9ACM2" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ACM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ACM2" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 456308, "stop": 456589, "observations": { "mutation": [ 456517 ], "stop": [ 456517 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3G4", "query_cov": 1.0, "subject_cov": 0.7096774193548387, "identity": 1.0, "score": 125.0, "evalue": 3.01e-36, "gene_start": 1, "gene_end": 66, "reference_start": 28, "reference_end": 93, "reference_sequence": "MQRIINKNMIYGGFMPLVSGALGFWNKMLFGTIIANTAISMIGGITNIVMASSSQQNHDSQDIDSIKIETINVDMSTIVGPKIPYMNIYYLNS" }, "paralog": false, "identity": 0.989247311827957, "score": 181.0, "evalue": 5.51e-61, "description": "Internal stop codon at 456517. Nonsense mutation around 456517.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3G4", "uniref50_id": "UniRef50_A0AAC9T3G4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3G4", "UniRef:UniRef50_A0AAC9T3G4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T3G4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3G4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7195.2036, "isoelectric_point": 4.351502418518066 }, "id": "GPKLONFDEH_807", "locus": "GCA000006625_00784" }, { "type": "cds", "sequence": "contig_1", "start": 456591, "stop": 456770, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T107", "UniRef:UniRef90_A0AAC9T107" ], "nt": "ATGTTAGGTCAAACAGTTGTTGGATTCTTAGGAATGATTTCACAATTAGTTTATGCTTTTAACCCTCAACCAAAATTTAATCCTCAACAAATGATCGATAACTCTGTTGCTCGCACTTATGCAAGAATCGCACAAAAACCAATGAATTCAACGATTAGTTTAGGAACTCCATTTTTTTAA", "aa": "MLGQTVVGFLGMISQLVYAFNPQPKFNPQQMIDNSVARTYARIAQKPMNSTISLGTPFF", "aa_hexdigest": "bea4dc0a718c88a5afcf32014c28ca8c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 456577, "stop": 456869, "observations": { "deletion": [ 456596 ], "mutation": [ 456794 ], "stop": [ 456794 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T107", "query_cov": 1.0, "subject_cov": 0.6082474226804123, "identity": 0.966, "score": 116.0, "evalue": 1.15e-32, "gene_start": 1, "gene_end": 59, "reference_start": 34, "reference_end": 92, "reference_sequence": "MQKISNNFKKVYYGGFSTSNSFNYMWNNALISSMLGQTVVGFLGMISQLVYAFNPQPKFRPQQMIDNSVAHTYARIAQKPMNSTISLGTPFFLICKE" }, "paralog": false, "identity": 0.9587628865979382, "score": 182.0, "evalue": 3.49e-61, "description": "Internal stop codon at 456794. Frameshift due to deletion around 456596. Nonsense mutation around 456794.", "psc": { "uniref90_id": "UniRef90_A0AAC9T107", "uniref50_id": "UniRef50_A0AAC9T107", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T107", "UniRef:UniRef50_A0AAC9T107" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T107", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T107" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6566.606100000001, "isoelectric_point": 9.991971015930176 }, "id": "GPKLONFDEH_808", "locus": "GCA000006625_00785" }, { "type": "cds", "sequence": "contig_1", "start": 456919, "stop": 457101, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAAAAATGATTGCAATTATTGAAGAACAATTTAACGTTAAAATCCCGTTAGGAAGTTCAAATCCTTTAGTTAATGACGTCGCTAGTAATATTTTTCAAAAATTTGGCTTAAAAATTTTAAAACAAGTCGCTAAAGAACACTTTAGTAACTTTCAACAAATTATTAAAAATAAATAA", "aa": "MFLKMIAIIEEQFNVKIPLGSSNPLVNDVASNIFQKFGLKILKQVAKEHFSNFQQIIKNK", "aa_hexdigest": "ec0198ac00232ad2a49ef8d5f54eb74e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6891.1075, "isoelectric_point": 9.778580665588379 }, "id": "GPKLONFDEH_809", "locus": "GCA000006625_00786" }, { "type": "cds", "sequence": "contig_1", "start": 457213, "stop": 457599, "strand": "-", "frame": 1, "gene": "rnhC", "product": "Ribonuclease HIII", "db_xrefs": [ "COG:COG1039", "COG:L", "EC:3.1.26.4", "GO:0000287", "GO:0003723", "GO:0004523", "GO:0005737", "GO:0006298", "GO:0032299", "GO:0043137", "SO:0001217", "UniRef:UniRef50_Q9PQ93", "UniRef:UniRef90_Q9PQ93" ], "nt": "ATGAGCCAATCAGAAATTTATGAAAGTGCCCAAGTAATTATTGGGTCTGATGAGGTTGGAGTTGGCGATTTATTTGGAGGTATAGTTGTTTGCGCAGTGAGTTTAAAAAAAAGTGATTTTAAAAAAATAAATCATTTAAAAATCATAGATTCTAAAAAACTCAATGATCAGCAAATGCAAGAAATATATCAACAAATTAAAAATCAGATTTCATATACAATTGTTTCTTATAATCCTAAAGAATACAATGAATTAATTAAAGAATACAATAATGCACATATTCTTAAAACTATTTTACATTATAAAGCATTACAAAGTGAAATTCACAAACACGCTAAATATTCAATTTTTAGTGTTGTTGATGCTTTTAGTTCATTAAAAAACTGA", "aa": "MSQSEIYESAQVIIGSDEVGVGDLFGGIVVCAVSLKKSDFKKINHLKIIDSKKLNDQQMQEIYQQIKNQISYTIVSYNPKEYNELIKEYNNAHILKTILHYKALQSEIHKHAKYSIFSVVDAFSSLKN", "aa_hexdigest": "a0ca1849fa8ae7a5c8643cd670a0477f", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnhC" ], "truncated": "both", "pseudogene": { "start": 456919, "stop": 457869, "observations": { "mutation": [ 457215, 457812 ], "stop": [ 457215, 457812 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ93", "query_cov": 1.0, "subject_cov": 0.4050632911392405, "identity": 1.0, "score": 246.0, "evalue": 1.02e-79, "gene_start": 1, "gene_end": 128, "reference_start": 91, "reference_end": 218, "reference_sequence": "MAIVSVKLTSNQVDKFLILWRPYLVHNNHNYAKYTFKIDGTLIIIYNTNTCTINTHDINSFLNTFLKREDLTLFNVIKQQVNTKKNNQNVMSQSEIYESAQVIIGSDEVGVGDLFGGIVVCAVSLKKSDFKKINHLKIIDSKKLNDQQMQEIYQQIKNQISYTIVSYNPKEYNELIKEYNNAHILKTILHYKALQSEIHKHAKYSIFSVVDAFSSLKNWNQYLQKVNFQPYEPNLLIPKAESIYTSVALASIIARVMFLKMIAIIEEQFNVKIPLGSSNPLVNDVASNIFQKFGLKILKQVAKEHFSNFQQIIKNK" }, "paralog": false, "identity": 0.9936708860759493, "score": 613.0, "evalue": 3.93e-227, "description": "Internal stop codon at 457215, 457812. Nonsense mutation around 457215, 457812.", "psc": { "uniref90_id": "UniRef90_Q9PQ93", "gene": "rnhC", "product": "Ribonuclease HIII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_Q9PQ93", "cog_id": "COG1039", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0032299", "GO:0000287", "GO:0003723", "GO:0004523", "GO:0043137", "GO:0006298" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ93", "EC:3.1.26.4", "UniRef:UniRef50_Q9PQ93", "COG:COG1039", "COG:L", "GO:0005737", "GO:0032299", "GO:0000287", "GO:0003723", "GO:0004523", "GO:0043137", "GO:0006298" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ93", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ93" ], "product": "Ribonuclease HIII" } }, "id": "GPKLONFDEH_810", "locus": "GCA000006625_00787" }, { "type": "cds", "sequence": "contig_1", "start": 457969, "stop": 458574, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_006688702.1", "SO:0001217", "UniParc:UPI00000C1C53", "UniRef:UniRef100_A0AAC9X6Y3", "UniRef:UniRef50_A0AAC9X6Y3", "UniRef:UniRef90_A0AAC9X6Y3" ], "nt": "ATGAAATTTAAGAAGTTTGATAGTAAAAAATCGATTTTACGTTTTTTACAAGTTGATGAAACAAAACGTGAAATTAATTTGTCTTTTAATGACACAACTGATTTTATTTTTTTTCTAAAAGAAATTGCTGAAGATCCTAATATTGCTAAAAAGAATGCTAAAAAATTAACTAGCTTATACATTGAAATTAGTAATAAACTGGGTAATGATGACAACACTGATTTAAGCGTTTATGAAACAAAATGTAATAATTTAATTGAAGCCTGTGTTATTGATGAAGCAATCTATTTAGTTAATATGGCAAAGAACATAGTCATTTTCTTAGCTCATAACAAACTTGATCTTTTACAAAAAGATTTTTTTGATAAATTATTATTAATAGAATCGCATTTTAATTTTGATCAAAATCTTACTTATTCATATATTAAAAACTCATCAATTGCGTTATTGATATTTCATTTAAAGCAATTTATTAACATTTATGATGAATTTTTAAACAATTTTGAAAATGTTATTAATCATAGTTTAGAAAATTCAATTTCAACTAAAAATAAATTAACCCAACAAATCAATCATTTTAATACTATAAAACGTCGTCCTTCATAA", "aa": "MKFKKFDSKKSILRFLQVDETKREINLSFNDTTDFIFFLKEIAEDPNIAKKNAKKLTSLYIEISNKLGNDDNTDLSVYETKCNNLIEACVIDEAIYLVNMAKNIVIFLAHNKLDLLQKDFFDKLLLIESHFNFDQNLTYSYIKNSSIALLIFHLKQFINIYDEFLNNFENVINHSLENSISTKNKLTQQINHFNTIKRRPS", "aa_hexdigest": "2757d3ca09721a74467c4db4b5d42efa", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1C53", "ncbi_nrp_id": "WP_006688702.1", "uniref100_id": "UniRef100_A0AAC9X6Y3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C53", "RefSeq:WP_006688702.1", "UniRef:UniRef100_A0AAC9X6Y3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6Y3", "uniref90_id": "UniRef90_A0AAC9X6Y3" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X6Y3", "uniref50_id": "UniRef50_A0AAC9X6Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23615.80770000003, "isoelectric_point": 6.964447593688964 }, "id": "GPKLONFDEH_811", "locus": "GCA000006625_00788" }, { "type": "cds", "sequence": "contig_1", "start": 458766, "stop": 458918, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGGTGCAAGTTATTCAATATCAGGCGATTTTGTTTATGTTGGTCTATTAGCGGGTAATGCAGCAATGCGTCGTCGTAATAATAGTTTCACATACGGAATATTAAATTCAGGGATATATGGAATGTTGTCAACATTAATTACTTATTGA", "aa": "MVGASYSISGDFVYVGLLAGNAAMRRRNNSFTYGILNSGIYGMLSTLITY", "aa_hexdigest": "a4b25d1b663b30269ebb40567f688b7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5387.129399999998, "isoelectric_point": 9.39608974456787 }, "id": "GPKLONFDEH_812", "locus": "GCA000006625_00789" }, { "type": "cds", "sequence": "contig_1", "start": 459004, "stop": 459126, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTTTGTATGAGTTAAATAATAATCATAATAAGCCTAAAATAACAAAGCTTGCTGCAATAATTAATGAATTTAATGGTGCTTTTTGTACTGACCCTACAATATGGCCAACAACATAA", "aa": "MFSLYELNNNHNKPKITKLAAIINEFNGAFCTDPTIWPTT", "aa_hexdigest": "8f8d397a2d52e06897065f2980760b22", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4569.176500000001, "isoelectric_point": 6.510758781433105 }, "id": "GPKLONFDEH_813", "locus": "GCA000006625_00790" }, { "type": "cds", "sequence": "contig_1", "start": 459147, "stop": 459266, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATATTTCTTTGTTTCACGTCAAAAAGAAAACTTTAATTACAAAAAACTAATTTTAATAGGAGTTATTTTAACATTCTTTTTAATTGCAATTGCACAAAGTATTCGTGGTTGA", "aa": "MVLYFFVSRQKENFNYKKLILIGVILTFFLIAIAQSIRG", "aa_hexdigest": "731c41a2692460990a13f3257a234202", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4595.5361, "isoelectric_point": 10.165906715393067 }, "id": "GPKLONFDEH_814", "locus": "GCA000006625_00791" }, { "type": "cds", "sequence": "contig_1", "start": 459498, "stop": 459713, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGAAATTTAATTTTTTTAAAGATAAACAAAATATATTAGGACAAAATCACATCTTAGAATTAAATAAAAAAAGTTTTATTCCATATCATTTTAAACCTTTGTTAATAACAATAACTTTATTGATTGCTTTATTAGTTGTCATTATTTTAATTTTTTCTGTTACCTTATCTAATAAGTTAGGCTTTAGTGAATTAGCTAAAGTAATATGA", "aa": "MKMKFNFFKDKQNILGQNHILELNKKSFIPYHFKPLLITITLLIALLVVIILIFSVTLSNKLGFSELAKVI", "aa_hexdigest": "19a54b5e00e7a8a90e63c90bb80eaa98", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8202.971699999993, "isoelectric_point": 10.000222969055176 }, "id": "GPKLONFDEH_815", "locus": "GCA000006625_00792" }, { "type": "cds", "sequence": "contig_1", "start": 459827, "stop": 460087, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCGCCATATTATACAATCATTGCTAAAGATTATTTATTAGCAAAATCTTTAGGAATTAAAGTTAATTTGATTTTTTGGCTAACAGCTTTTTTTGCAGTTGTCGCAACTGTTTCAAGCATCATTTTAATTGGTGTTTTAACACTATTAGGAATGATAGCGCCTCATGTGGCACGAATATTAAATCCAAAAGGCAATAGTTTTAAACAATTATTATTATCATTTGTAATTTCATTATTACTATTAACATCTTCACGTTGA", "aa": "MAPYYTIIAKDYLLAKSLGIKVNLIFWLTAFFAVVATVSSIILIGVLTLLGMIAPHVARILNPKGNSFKQLLLSFVISLLLLTSSR", "aa_hexdigest": "23429a59a8a72dbded9f730da4c9bab2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9407.386499999993, "isoelectric_point": 10.214580345153806 }, "id": "GPKLONFDEH_816", "locus": "GCA000006625_00793" }, { "type": "cds", "sequence": "contig_1", "start": 460145, "stop": 460636, "strand": "-", "frame": 3, "gene": "fepD", "product": "Ferrichrome ABC transporter (Permease)", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_Q9PQ90", "UniRef:UniRef90_Q9PQ90" ], "nt": "GTGCTAATTTGTTATTGTCTTTTTGATGGTAAAGAGTTAATGAATTTTAAAAATGTAGGTGAAGAAATAAATGAGGGAAGTGCCCCTTATATTCGTAAATTTATTGCTACTCCAATTGCAATCTTTTTATCTGCATTAACATTATCATTTAGTGGGTACAGTATGCAAGTAGTTTCACGAAACCCACTAGCTTCACCAACTACATTAGGATATTTACCTGCTGCAATTTTAGGTTTAGCAATTAGTAAACTTGCTATTAATCAAATTTTATATCTTCCTTTCATTATTGGTATTGTTTTTGCTTCATGTCTAATTGTAATTAATTTTTTCTTGGTAAAAGGAAATGCTTTAGAAGCAAGTTTTAAACCAATTTTAGTAGGATTTGCAATTGGAGGTATCATTACTGGTATTAATGTATTATTAGAAGACCTTGTAAAAGATATAAACATTAAAATTACAGGTTTCGTTGAACCTCCAATTAATTTTCAATGA", "aa": "MLICYCLFDGKELMNFKNVGEEINEGSAPYIRKFIATPIAIFLSALTLSFSGYSMQVVSRNPLASPTTLGYLPAAILGLAISKLAINQILYLPFIIGIVFASCLIVINFFLVKGNALEASFKPILVGFAIGGIITGINVLLEDLVKDINIKITGFVEPPINFQ", "aa_hexdigest": "46a47780a0bf48e601e74b2bc7b1be1e", "start_type": "GTG", "rbs_motif": null, "genes": [ "fepD" ], "truncated": "both", "pseudogene": { "start": 459842, "stop": 460735, "observations": { "mutation": [ 460147, 460693 ], "stop": [ 460147, 460693 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ90", "query_cov": 1.0, "subject_cov": 0.4808259587020649, "identity": 0.9940000000000001, "score": 310.0, "evalue": 5.74e-104, "gene_start": 1, "gene_end": 163, "reference_start": 34, "reference_end": 196, "reference_sequence": "MFKNKSLKFKNKLDWKTHFRRLITSLLIILSICVLICYCLFDGKELMNFKNVGEEINEGSAPYIRKFIATPIAIFLSALTLSFSGYSMQVVSRNPLASPTTLGYLPAAILGLAISKLAINQILYLPFIIGIVFASCLIVINFFLVKGNALEASFKPILVGFAIGGIITGINVLLEDLVKDINIKITGFVEPPINFQWQQLCIGGPLIIISSLANLFMAPYYTIIAKDYLLAKSLGIKVNLIFWLTAFFAVVATVSSIILIGVLTLLGMIAPHVARILNPKGNSFKQLLLSFVISLLLLTSSRWLINVYNQFDINLFSAIAALPVFAYIFVSKQYRRNVE" }, "paralog": false, "identity": 0.9932659932659933, "score": 564.0, "evalue": 1.17e-206, "description": "Internal stop codon at 460147, 460693. Nonsense mutation around 460147, 460693.", "psc": { "uniref90_id": "UniRef90_Q9PQ90", "gene": "FepD", "product": "Ferrichrome ABC transporter (Permease)", "uniref50_id": "UniRef50_Q9PQ90", "cog_id": "COG0609", "cog_category": "P", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ90", "UniRef:UniRef50_Q9PQ90", "COG:COG0609", "COG:P" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ90" ], "product": "Ferrichrome ABC transporter (Permease)" } }, "id": "GPKLONFDEH_817", "locus": "GCA000006625_00794" }, { "type": "cds", "sequence": "contig_1", "start": 460779, "stop": 461126, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCATTGGGAATTCAACCAGACTATGCTTCATTCGTTAAAGAAAAAGGAAAAACATATACTAAACTACCAGGATTTTTAGCTGATTTAGTAGATGAAACAAAAACCGAATTTAAAGATTTCACTGGTGATGCTCAAAAAGTTGCTGGTATGAATTTATATGCTCTAGGTGGTAATAATTCTCATATTAACCGTTCTTTTAAAACTCTTATTGGCAATGCAATACCAAAAGCTGTTCCTACTGAACGTGGCGATTCAAATAATTCTCGTTCACCAATTAATTTTGAATTCTATGATCGTACAACAAACACATTACAATCTGTTGAAGGAACAAAAGTTGTAAGTTGA", "aa": "MALGIQPDYASFVKEKGKTYTKLPGFLADLVDETKTEFKDFTGDAQKVAGMNLYALGGNNSHINRSFKTLIGNAIPKAVPTERGDSNNSRSPINFEFYDRTTNTLQSVEGTKVVS", "aa_hexdigest": "bf6f46aba7dd3528870c302ea6b1cb1d", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12596.967899999985, "isoelectric_point": 7.931303596496583 }, "id": "GPKLONFDEH_818", "locus": "GCA000006625_00795" }, { "type": "cds", "sequence": "contig_1", "start": 461319, "stop": 461507, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATCAATGCAAGAAGCTTTTTATGGAACTGCGGATTATGTTTTCTTTAATTATGACTCAAACAATCTTGAAGGCAATGAAGATGGAGAGATTTTAAAATCAAATTTTGAAAAATTCTTTAAGAAAACTGTTAATAATAACAAAATTATTCCAGTTGATTTTCAATTTGAATATTCTTCAATTTGA", "aa": "MLSMQEAFYGTADYVFFNYDSNNLEGNEDGEILKSNFEKFFKKTVNNNKIIPVDFQFEYSSI", "aa_hexdigest": "b257a0a80c8681b5415a649de8f46730", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7292.980200000001, "isoelectric_point": 4.31762638092041 }, "id": "GPKLONFDEH_819", "locus": "GCA000006625_00796" }, { "type": "cds", "sequence": "contig_1", "start": 461739, "stop": 462251, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTGTTTGTCAAAACAAAACAATATTATTTTAAAAATGAGGATAATAAAAATATGTTTAAGAAAAATAAAAAATCTTTAGCAGTATTGCTAGTAGGTTTTTCAACTGTTTTAATTTCAAGTATTGCGGTTGCTGCTACAGCATGTGCAAGAAAAGACAATAACGTTAAAGAGGAAGGAAAAACAACACTTAATAAGTTAATTTCAAAAAAAGCTCCAAAACAAAATGCAACTAAATCTAGTGAAAAATATATCTTAAGTGATGTTAATAATAGTAGTACTTTTTTTAATTCACTAACAGACCCTTATAAATGTTTTGAAATGTTTGCAAAATCATTGGATGATGCAGGTTATGAAATTACATCACATGAACCTTTGAAAAATAGCGGTAAAACAAGTTTTTTAGAGTATGCTAAAGAACTTAATAATAAGATTCGTGAATATGTTAGAAAAGTTGCTAATTTACCAACTATTAAAAAATTAAGAGATAATAATACAACTGTTTTATGA", "aa": "MKLFVKTKQYYFKNEDNKNMFKKNKKSLAVLLVGFSTVLISSIAVAATACARKDNNVKEEGKTTLNKLISKKAPKQNATKSSEKYILSDVNNSSTFFNSLTDPYKCFEMFAKSLDDAGYEITSHEPLKNSGKTSFLEYAKELNNKIREYVRKVANLPTIKKLRDNNTTVL", "aa_hexdigest": "631ad42b67df289ed960695f42fd8e63", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19284.000100000012, "isoelectric_point": 9.69799518585205 }, "id": "GPKLONFDEH_820", "locus": "GCA000006625_00797" }, { "type": "cds", "sequence": "contig_1", "start": 462275, "stop": 463024, "strand": "-", "frame": 3, "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "db_xrefs": [ "COG:COG0482", "COG:J", "EC:2.8.1.13", "GO:0000049", "GO:0002143", "GO:0005524", "GO:0005737", "GO:0008033", "GO:0016740", "GO:0016783", "GO:0103016", "KEGG:K00566", "SO:0001217", "UniRef:UniRef50_Q9PQ88", "UniRef:UniRef90_Q9PQ88" ], "nt": "ATGGGTCATTATGCAAGAGTTAAGCATGATAGGAATTCATCTTTTTTACTAAAAGCTATTGATACAAATAAGGATCAAACTTATTTTTTGTGTAATTTAAACCAAAATCAACTACAGAATGTGCTATTTCCTATTGGGCATTTAACTAAACTACAGGTGCGCGCTATTGCTAAAAAACACGGTTTGATTACAGCTAATAAGAAAGATTCAACAGGGATTTGTTTTATTGGTGAACGTAATTTTAAAACTTTTTTACAAAATTATATTCCTAACCAACCAGGGCAAATTATAAATATTGTCAATAATCAAATTATTGGTCATCACATAGGAACAATGTATTATACAATTGGACAACGAAAAGGATTGAATCTAGGTGGCATGAATGAGAGAATGTTTGTTTGTGATAAAGATATCGATAAAAAAATAATCTACGTTGCCCCTTCAAGTTTTGAAAAACAATATTTAATTTCAACTCAAGCATTAATTGAAAATATAAATTTTATTGAACCATATAATCCTCAAATTCCAATTATGGTACGCTTTCGTCACCGACAAGATTTAATAATAGTAAACGATTTTTTACCTATTAAAAATACCAAAAATGTACTAATAAATTATGAGTCAGCTAGAGCTATTACTCCTGGGCAATATGCTGTCTTTTACCAAAATGATCATTGCATTGGCGGTGGAATTGTTTCTAAAACTAATATTGGACATCAAAAAGTGGATTTTTTGGTATATAAATCATAG", "aa": "MGHYARVKHDRNSSFLLKAIDTNKDQTYFLCNLNQNQLQNVLFPIGHLTKLQVRAIAKKHGLITANKKDSTGICFIGERNFKTFLQNYIPNQPGQIINIVNNQIIGHHIGTMYYTIGQRKGLNLGGMNERMFVCDKDIDKKIIYVAPSSFEKQYLISTQALIENINFIEPYNPQIPIMVRFRHRQDLIIVNDFLPIKNTKNVLINYESARAITPGQYAVFYQNDHCIGGGIVSKTNIGHQKVDFLVYKS", "aa_hexdigest": "4a2d77648f55814326e30c0f3af420ae", "start_type": "ATG", "rbs_motif": null, "genes": [ "mnmA" ], "truncated": "3-prime", "pseudogene": { "start": 462275, "stop": 463324, "observations": { "mutation": [ 463165 ], "stop": [ 463165 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ88", "query_cov": 1.0, "subject_cov": 0.6467532467532467, "identity": 1.0, "score": 509.0, "evalue": 1.06e-180, "gene_start": 1, "gene_end": 249, "reference_start": 137, "reference_end": 385, "reference_sequence": "MEVKVKKRVVVGLSGGVDSSVSALLLKQEGYEVIGLFMSNWDTIANFENNHEFNKTHQGCESELDYQDAQAVAQKIGIPLYRVEFIKEYWDNVFEYFLSEYQKNRTPNPDILCNQFIKFDSFLNYAKNELQADYIAMGHYARVKHDRNSSFLLKAIDTNKDQTYFLCNLNQNQLQNVLFPIGHLTKLQVRAIAKKHGLITANKKDSTGICFIGERNFKTFLQNYIPNQPGQIINIVNNQIIGHHIGTMYYTIGQRKGLNLGGMNERMFVCDKDIDKKIIYVAPSSFEKQYLISTQALIENINFIEPYNPQIPIMVRFRHRQDLIIVNDFLPIKNTKNVLINYESARAITPGQYAVFYQNDHCIGGGIVSKTNIGHQKVDFLVYKS" }, "paralog": false, "identity": 0.997134670487106, "score": 716.0, "evalue": 1.88e-264, "description": "Internal stop codon at 463165. Nonsense mutation around 463165.", "psc": { "uniref90_id": "UniRef90_Q9PQ88", "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "ec_ids": [ "2.8.1.13" ], "uniref50_id": "UniRef50_Q9PQ88", "cog_id": "COG0482", "cog_category": "J", "kegg_orthology_id": "K00566", "go_ids": [ "GO:0000049", "GO:0002143", "GO:0005524", "GO:0005737", "GO:0008033", "GO:0016740", "GO:0016783", "GO:0103016" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ88", "EC:2.8.1.13", "UniRef:UniRef50_Q9PQ88", "COG:COG0482", "COG:J", "KEGG:K00566", "GO:0000049", "GO:0002143", "GO:0005524", "GO:0005737", "GO:0008033", "GO:0016740", "GO:0016783", "GO:0103016" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ88" ], "product": "tRNA-specific 2-thiouridylase MnmA" } }, "id": "GPKLONFDEH_821", "locus": "GCA000006625_00798" }, { "type": "cds", "sequence": "contig_1", "start": 463163, "stop": 463432, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGGAAGTTAAAGTAAAAAAACGTGTCGTTGTTGGCTTGTCTGGTGGCGTAGATTCTTCTGTTAGTGCTTTATTATTAAAACAAGAAGGCTATGAAGTGATTGGTTTATTTATGAGTAATTGGGATACAATTGCAAATTTTGAAAATAACCATGAATTTAATAAAACGCATCAGGGTTGTGAGAGTGAATTAGATTACCAAGATGCTCAGGCTGTTGCTCAAAAGATCGGTATTCCATTATATCGTGTTGAATTTATTAAAGAATATTGA", "aa": "MEVKVKKRVVVGLSGGVDSSVSALLLKQEGYEVIGLFMSNWDTIANFENNHEFNKTHQGCESELDYQDAQAVAQKIGIPLYRVEFIKEY", "aa_hexdigest": "360c470393423fc673e3bf97fd191c01", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4R2K1R8", "query_cov": 0.9325842696629213, "subject_cov": 0.9120879120879121, "identity": 0.506, "score": 70.5, "evalue": 4.21e-14, "valid": false, "product": "tRNA methyltransferase", "uniref50_id": "UniRef50_J9FDV7" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 79, "aa_cov": 0.8876404494382022, "hmm_cov": 0.7974683544303798, "evalue": 4.562025176843857e-09, "score": 31.331361770629883, "start": 6, "stop": 84 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 83, "aa_cov": 0.9325842696629213, "hmm_cov": 0.9156626506024096, "evalue": 2.387425320036072e-23, "score": 78.44087219238281, "start": 7, "stop": 89 } ], "seq_stats": { "molecular_weight": 10054.275399999993, "isoelectric_point": 5.045563316345214 }, "id": "GPKLONFDEH_822", "locus": "GCA000006625_00799" }, { "type": "cds", "sequence": "contig_1", "start": 463541, "stop": 464062, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T243", "UniRef:UniRef90_A0AAC9T243" ], "nt": "ATGAAAAAAGCAATTTTGATTTCCATTTCTTCATTAATTGGGATTAGTGCGATTGTCGCAACTAGTACTTTTTTGTCATTATCAGCCAAAACAAATATTTATGCAAAATTAATAAAATCAAGCGATCTTAATCAAAGTTTAAATTACACTAATTTATCAGATGCTAATATTGGAATTAAAGAAATGCTTTTAGGTACTAATAAAATTAATAATGGAAATTATGTTTTATATATTGGAACACAATCAAACCTTGATAATTTAAATTTTGTTTATGATAATCAAACTAACCATATTAATTCGATTAGTGATTTAAAATATAATGATAATTTGAAGTTTAATGGATCATTAGCAAAATCAATTAATAATGTCAAAAATTATGCAGATAAAGGTGTTTATGAGCATGTCCCAGAATTCTATAGTTTTATTGATTTAATGGATACTAATGTTTTTAAACAAAAACAAGATTATGAAAATTTAATTAAACAAAATAAATCAAGCACTATTAAAGAAGATCAAAATTGA", "aa": "MKKAILISISSLIGISAIVATSTFLSLSAKTNIYAKLIKSSDLNQSLNYTNLSDANIGIKEMLLGTNKINNGNYVLYIGTQSNLDNLNFVYDNQTNHINSISDLKYNDNLKFNGSLAKSINNVKNYADKGVYEHVPEFYSFIDLMDTNVFKQKQDYENLIKQNKSSTIKEDQN", "aa_hexdigest": "87c84c1701d0370c050e70194739b1c3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 463541, "stop": 464365, "observations": { "mutation": [ 464060 ], "stop": [ 464060 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T243", "query_cov": 1.0, "subject_cov": 0.5805369127516778, "identity": 1.0, "score": 319.0, "evalue": 3.52e-108, "gene_start": 1, "gene_end": 173, "reference_start": 1, "reference_end": 173, "reference_sequence": "MKKAILISISSLIGISAIVATSTFLSLSAKTNIYAKLIKSSDLNQSLNYTNLSDANIGIKEMLLGTNKINNGNYVLYIGTQSNLDNLNFVYDNQTNHINSISDLKYNDNLKFNGSLAKSINNVKNYADKGVYEHVPEFYSFIDLMDTNVFKQKQDYENLIKQNKSSTIKEDQNWANNAPTDYSFDVNKKYKDKSGREVYFRNDTQAVKFREILNFLKKYLSKEKLIELSTSQTPGIVLFYSQDNLSKGPQVYASSKIYDKSKNERKESYVSGDNVYNSSRFEFGGDLNAAIYSVYGKK" }, "paralog": false, "identity": 0.9963503649635036, "score": 536.0, "evalue": 3.53e-196, "description": "Internal stop codon at 464060. Nonsense mutation around 464060.", "psc": { "uniref90_id": "UniRef90_A0AAC9T243", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAC9T243", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T243", "UniRef:UniRef50_A0AAC9T243" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T243", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T243" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_823", "locus": "GCA000006625_00800" }, { "type": "cds", "sequence": "contig_1", "start": 464252, "stop": 464437, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTATTCTCAAGACAATCTTAGCAAGGGTCCACAAGTTTATGCTAGTTCAAAAATATATGACAAATCAAAAAATGAACGTAAAGAATCATATGTTTCTGGGGACAACGTCTATAATTCATCACGTTTTGAATTTGGTGGAGATTTAAACGCTGCTATTTATAGTGTCTATGGTAAAAAATAA", "aa": "MFYSQDNLSKGPQVYASSKIYDKSKNERKESYVSGDNVYNSSRFEFGGDLNAAIYSVYGKK", "aa_hexdigest": "95d0bbfbfecc6f33c7230434f1d155b7", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6932.518400000004, "isoelectric_point": 8.891302299499515 }, "id": "GPKLONFDEH_824", "locus": "GCA000006625_00801" }, { "type": "cds", "sequence": "contig_1", "start": 464926, "stop": 465474, "strand": "-", "frame": 1, "gene": "iS285", "product": "Mutator family transposase", "db_xrefs": [ "COG:COG3328", "COG:X", "SO:0001217", "UniRef:UniRef50_Q9PQ86", "UniRef:UniRef90_Q9PQ86" ], "nt": "ATGGACAAAAAGAATATTTTTACAATTCTAGAGAAGTTAGTTAAAAAATACAAGTATTTGCACAAACCAATTCTAGATGACTATATTAGTACTTTTACAAGAACCTTTTTAGAATATATATTGACAATCGAAATGAAATATCATTTAAATTATGAACGTTTTGAACGTTATTTAAGTAGTAATTCTAAAAACTATCGTAATGGTTTTTCTAAAAAACAACTGTATATGAACGATTTAAAAGTTTCAATTAATATTCCACGTGATCGTAATGGAGCTTTTGAATCAAAAATTATTTCAAAATACCAAGTTGATATTTCACATTTTGAATATCGCTTATTATCGTTGGGAATCAATAATTTACAATTAAATGAATATGAAAAAATATTAGAAGATATTTATAATATTTATAATGACAAAATGGTTGATAAACTATGTAATGAAATTGCTTTAAAAATTTTTGATTATTTAGAGAATAAAAAACAAGAACTAAATAATATAGATGTTGATCAAATTTTTATCGATGGTTTTAATTTATCATCAAATAAATGA", "aa": "MDKKNIFTILEKLVKKYKYLHKPILDDYISTFTRTFLEYILTIEMKYHLNYERFERYLSSNSKNYRNGFSKKQLYMNDLKVSINIPRDRNGAFESKIISKYQVDISHFEYRLLSLGINNLQLNEYEKILEDIYNIYNDKMVDKLCNEIALKIFDYLENKKQELNNIDVDQIFIDGFNLSSNK", "aa_hexdigest": "ad152dbc0db9884a63a0f6e41332e6d5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "iS285" ], "truncated": "5-prime", "pseudogene": { "start": 464668, "stop": 465474, "observations": { "mutation": [ 464841, 464844, 464871, 464928 ], "stop": [ 464841, 464844, 464871, 464928 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ86", "query_cov": 1.0, "subject_cov": 0.6791044776119403, "identity": 1.0, "score": 361.0, "evalue": 4.65e-125, "gene_start": 1, "gene_end": 182, "reference_start": 1, "reference_end": 182, "reference_sequence": "MDKKNIFTILEKLVKKYKYLHKPILDDYISTFTRTFLEYILTIEMKYHLNYERFERYLSSNSKNYRNGFSKKQLYMNDLKVSINIPRDRNGAFESKIISKYQVDISHFEYRLLSLGINNLQLNEYEKILEDIYNIYNDKMVDKLCNEIALKIFDYLENKKQELNNIDVDQIFIDGFNLSSNKWLTIVITKDLEKRLTILGFWVINNLEDKWWNDIIIKISKYHPQVINYKVIFKDNDELLISLKNHTSNNFERARFVFINKSILNQKN" }, "paralog": false, "identity": 0.9813432835820896, "score": 519.0, "evalue": 1.18e-189, "description": "Internal stop codon at 464841, 464844, 464871, 464928. Nonsense mutation around 464841, 464844, 464871, 464928.", "psc": { "uniref90_id": "UniRef90_Q9PQ86", "gene": "IS285", "product": "Mutator family transposase", "uniref50_id": "UniRef50_Q9PQ86", "cog_id": "COG3328", "cog_category": "X", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ86", "UniRef:UniRef50_Q9PQ86", "COG:COG3328", "COG:X" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ86" ], "product": "Mutator family transposase" } }, "id": "GPKLONFDEH_825", "locus": "GCA000006625_00802" }, { "type": "cds", "sequence": "contig_1", "start": 465681, "stop": 466307, "strand": "-", "frame": 2, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "db_xrefs": [ "COG:COG0344", "COG:I", "EC:2.3.1.15", "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772", "SO:0001217", "UniRef:UniRef50_Q9PQ85", "UniRef:UniRef90_Q9PQ85" ], "nt": "ATGGATCAAGTATATAACATTGTCATGGCTTATATTCTTACATTAATCATATCTCCTTTATATTCGTATTTAATTGGTAGTTTAAATGCAAGCATCATTTTATCCTTAGTTTTTAAAAAACAAGATGTTCGTCTTTTTGCTTCTAAAAATGCAGGCATGACTAATATGATACGTGTTCACGGAAAAAAATTAGGTATATTAACGCTTTTTTTAGATATTATTAAACCAATCACCACAGTTAGTTTAACTTACATTATTTACAAATATGCTTTAGACGCTCCATTTGATTTAAGTAATGGTTTTAATCAAGCCATTTTAGTTTATTTTGGTGGTATTTTCACCATTATTGGTCATTGTTACCCAATCTTCTTTAAATTTCAAGGCGGGAAGGGAGTTGCATGTTATGGCGGTTTTTTAATTACAGTTGATCCAATAGTAGCGCTAATTGGAATCATTACACTATTGGTCATTTTACTAATTACTAAATATATGTCTTTATCTGCTATGATTACTGCAACTTTCACTTGTTTTTTAGTATTAGTTCCAGGAATTAATTACATACCATACTATAATCAAAATTTCACTACGTATTTTTTTGATTTAAATTATGTTATAAAAGGAATCTGA", "aa": "MDQVYNIVMAYILTLIISPLYSYLIGSLNASIILSLVFKKQDVRLFASKNAGMTNMIRVHGKKLGILTLFLDIIKPITTVSLTYIIYKYALDAPFDLSNGFNQAILVYFGGIFTIIGHCYPIFFKFQGGKGVACYGGFLITVDPIVALIGIITLLVILLITKYMSLSAMITATFTCFLVLVPGINYIPYYNQNFTTYFFDLNYVIKGI", "aa_hexdigest": "7be9138216a9cf234d3a167fec29b3f1", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PQ85", "query_cov": 1.0, "subject_cov": 0.8093385214007782, "identity": 1.0, "score": 393.0, "evalue": 2.94e-137, "valid": true, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "ec_ids": [ "2.3.1.15" ], "uniref50_id": "UniRef50_Q9PQ85", "cog_id": "COG0344", "cog_category": "I", "go_ids": [ "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ85" ], "product": "Glycerol-3-phosphate acyltransferase" }, "genes": [ "plsY" ], "id": "GPKLONFDEH_826", "locus": "GCA000006625_00803" }, { "type": "cds", "sequence": "contig_1", "start": 466522, "stop": 466791, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATAATAAGAAATTTATTAATAAAACATTTGATAATACTAACCAAATACGATTGCAAAATAATTTAAAAACTTATGCTCCTAAATTTTTAAATTTAATTATTATTAATAATATTTTTGTATTAATTATGATTATTAGTTTGTGTATTTTCTTTTCTGATCCACATTTTTTATATCGAAACGATGTCATTTCGAACCAATATCGCCAATTTATATTTACATATCAGCAAACAGCAATCGCAATTGTTTTATATGTTTTAGCATGA", "aa": "MINNKKFINKTFDNTNQIRLQNNLKTYAPKFLNLIIINNIFVLIMIISLCIFFSDPHFLYRNDVISNQYRQFIFTYQQTAIAIVLYVLA", "aa_hexdigest": "e30e35134d54b9eaa06e30c8a28485a3", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10611.406699999985, "isoelectric_point": 9.66582546234131 }, "id": "GPKLONFDEH_827", "locus": "GCA000006625_00804" }, { "type": "cds", "sequence": "contig_1", "start": 466798, "stop": 466926, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAATTTTTTCTTCTTTGGCTATTTATTAGAATATGTAAAATTAAATAAATTTGAAACATTTAATTTTGCATTAGCGTCAATTTTATTTAATCCTTATGCTTATGTTATTATTGTTAAAAATTGA", "aa": "MVNFFFFGYLLEYVKLNKFETFNFALASILFNPYAYVIIVKN", "aa_hexdigest": "fd80da2b9f13548249a4b0b022ce99e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5049.918600000002, "isoelectric_point": 8.06333484649658 }, "id": "GPKLONFDEH_828", "locus": "GCA000006625_00805" }, { "type": "cds", "sequence": "contig_1", "start": 467200, "stop": 467313, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTATTGGAATTGCATTCTTATATTTTATTAATCCAAAAGCACGTTGTACAAAAAACAATCGTGTTTTATTAATTGTTTTGTCTTACATTTTAATTATTAGTTCAATTTGA", "aa": "MCIGIAFLYFINPKARCTKNNRVLLIVLSYILIISSI", "aa_hexdigest": "a0c5bcfba06033e2e47b15a2eaf4f541", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4215.183799999999, "isoelectric_point": 9.696061134338382 }, "id": "GPKLONFDEH_829", "locus": "GCA000006625_00806" }, { "type": "cds", "sequence": "contig_1", "start": 467811, "stop": 468467, "strand": "-", "frame": 2, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "GO:0000774", "GO:0005737", "GO:0006457", "GO:0042803", "GO:0051087", "KEGG:K03687", "RefSeq:WP_006688590.1", "SO:0001217", "UniParc:UPI00000C1C59", "UniRef:UniRef100_Q9PQ83", "UniRef:UniRef50_Q9PQ83", "UniRef:UniRef90_Q9PQ83" ], "nt": "ATGAGCAAAAATAATGAAAACATCAAACACCAAAATAATGACAAAGTTAACAACCAAGTTGATAAAAAAGAAACTAAAAATCATAATAAGCAAGAATTTAAATATAAAGAATTATATGAACATGAATTAAAGAAAAATAAAGAATTACAAAATATCAATACACTTTTAAAAGATAAAAATCAACAGTTAGAAGAGCAAATTAGTCAATTAAATCAAGATTTTATCAAACAATTAGAAACAAAAGCAAAACAAGCACAACAAATTCTAGAACAAAAAGTTAATGAACTAGAAGCACGCCATGAAGCCAAAGTAAATGATGCTGTCTTTAAAATTTTCAAATTTAAAATGGAACCTTTATTAGATGCAATTAATCATTTTACGAAAATTGTAAATCAAAACTATGATGATCCTAAAATCCAAGCTTTCATTGAGGGGTTTAAAATGTTTTCTCAAAACATGATTGATGGTTTAGATAATCTAAAAATTACAAAAATATCTCCACAGGTTAATGATAGTTTAAATGACGAAATAATGGAAGTTTTTGAAGTTGTTGAAAACACAAACAAACCATCAATGCATGTAGTTGAAGTTATTAGTGATGGTTTTAAATATAATGATAAAGTTATTAAATTTGCAGTTGTAAAAGTTGCTAAATAA", "aa": "MSKNNENIKHQNNDKVNNQVDKKETKNHNKQEFKYKELYEHELKKNKELQNINTLLKDKNQQLEEQISQLNQDFIKQLETKAKQAQQILEQKVNELEARHEAKVNDAVFKIFKFKMEPLLDAINHFTKIVNQNYDDPKIQAFIEGFKMFSQNMIDGLDNLKITKISPQVNDSLNDEIMEVFEVVENTNKPSMHVVEVISDGFKYNDKVIKFAVVKVAK", "aa_hexdigest": "1cc3739bdd518632e128a3134d757d1e", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "ups": { "uniparc_id": "UPI00000C1C59", "ncbi_nrp_id": "WP_006688590.1", "uniref100_id": "UniRef100_Q9PQ83", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C59", "RefSeq:WP_006688590.1", "UniRef:UniRef100_Q9PQ83" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ83", "uniref90_id": "UniRef90_Q9PQ83" }, "psc": { "uniref90_id": "UniRef90_Q9PQ83", "gene": "grpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_Q9PQ83", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687", "go_ids": [ "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0006457" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ83" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "GPKLONFDEH_830", "locus": "GCA000006625_00807" }, { "type": "cds", "sequence": "contig_1", "start": 468467, "stop": 469594, "strand": "-", "frame": 3, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "EC:1.8.4.-", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085", "RefSeq:WP_006689084.1", "SO:0001217", "UniParc:UPI00001295A0", "UniRef:UniRef100_Q9PQ82", "UniRef:UniRef50_Q9PQ82", "UniRef:UniRef90_Q9PQ82" ], "nt": "ATGGCAAAACGTGATTATTATGAAATTTTAGGTGTTAGTAAATCAGCAACTCCTGAAGAGATTAAAGCGGCATTTCGTAAATTAGCAAAAGAACACCATCCTGATCGTAATAAATCTGCTGATGATACTCTTTTTAAAGAAATTAATGAAGCATATGAGGTTTTATCTGATTCTAAAAAACGTGCACAATACGACCAATTTGGCCATGATGGTCCACAAGGTTTTAGCAGTGCTAGTGGGTTTTCTGGGTTTAGTGGAGGATTTGGTGGTGTTGATTTTGATATTAATGACATTTTTGGCAGTTTTTTTAATAATGCTTCTAGTTCACATAATTCATCAAACCAATATGAAACATATGATATTCACTTACGTTTACATTTAGATTTTATGGAAGCTGTTAATGGTATTTCTAAAAATATTAATTATGATCGTAAAATTACGTGTCATAAATGTCAAGGAACAGGTGCAAAAGATCCTAAAGATGTCAAAATTTGCACTAAATGTCACGGTAGAGGAACTAGCATTGAAAATGTTCATTCCTTATTTGGAACAATTCAACAAGAAGTTGAATGTCATGAGTGTGAAGGAACAGGTAAAGTTGCTAGTAGCAAATGTGAACAATGTTATGGTAAAAAAGTTATCAATGAACGTGTTAATTTAACTGTTGAAATCCCAGCAGGTACACGAGAAGGTGAAAAATTATTAGTAAGCAAAAAAGGTAATATTGTCAATAATCAAGAATTTGATCTTTATTTACACATTAGTGTTAAACCATCAAAGTATTTTGCATTAGATGGTTTAGATATTTATTCAGAAACTTACATTGATCCCATCAAAGCAATTGTTGGAGGAATAATTGAAGTAGTAACAATTAATGGTATTAAAACCATTGAAATTCCTTCAAATACTCCTGAAGGTAAAAAATTTAGAATTAGTGGATCAGGAATCGTTAATAAAAAATCAAATATTTTTGGTAAAAAGAATGGAGATTTTTATACTACAATTCGTTATGCAAAGCCAGTTGAATTATCCAAAGATGAAATTGCTTATTTAAAAAATATTTCTACTCGTACAAACCAAAATGTTGAGTATTATAAAAATAAAGTATTAAAGGAGATTAGTAAATAA", "aa": "MAKRDYYEILGVSKSATPEEIKAAFRKLAKEHHPDRNKSADDTLFKEINEAYEVLSDSKKRAQYDQFGHDGPQGFSSASGFSGFSGGFGGVDFDINDIFGSFFNNASSSHNSSNQYETYDIHLRLHLDFMEAVNGISKNINYDRKITCHKCQGTGAKDPKDVKICTKCHGRGTSIENVHSLFGTIQQEVECHECEGTGKVASSKCEQCYGKKVINERVNLTVEIPAGTREGEKLLVSKKGNIVNNQEFDLYLHISVKPSKYFALDGLDIYSETYIDPIKAIVGGIIEVVTINGIKTIEIPSNTPEGKKFRISGSGIVNKKSNIFGKKNGDFYTTIRYAKPVELSKDEIAYLKNISTRTNQNVEYYKNKVLKEISK", "aa_hexdigest": "c17f3981138183607df0f8e99bec84c6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00001295A0", "ncbi_nrp_id": "WP_006689084.1", "uniref100_id": "UniRef100_Q9PQ82", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001295A0", "RefSeq:WP_006689084.1", "UniRef:UniRef100_Q9PQ82" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ82", "uniref90_id": "UniRef90_Q9PQ82" }, "psc": { "uniref90_id": "UniRef90_Q9PQ82", "gene": "dnaJ", "product": "molecular chaperone DnaJ", "ec_ids": [ "1.8.4.-" ], "uniref50_id": "UniRef50_Q9PQ82", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ82" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "GPKLONFDEH_831", "locus": "GCA000006625_00808" }, { "type": "cds", "sequence": "contig_1", "start": 469722, "stop": 469901, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13276.12" ], "nt": "ATGGATTTAATTGAAAAAATTTGTAAAAGTCATAAGCATTATATTGGTTGTCGTAAAATTCAAAAAATTCTTGAAACACAATATAATGTTAAATTAAATTACAAAACAATTAATTTATATATGAATAAAATGGATTTATGCAAACCTTGTCATCATGAACATCATGAAAAAAAGGAATAA", "aa": "MDLIEKICKSHKHYIGCRKIQKILETQYNVKLNYKTINLYMNKMDLCKPCHHEHHEKKE", "aa_hexdigest": "fcc0b0bc3699ff416b7fd6afc5305fa6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13276.12", "name": "HTH_21", "length": 52, "aa_cov": 0.8813559322033898, "hmm_cov": 1.0, "evalue": 1.0902924068649726e-13, "score": 46.85664749145508, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 7241.514300000001, "isoelectric_point": 9.118102073669434 }, "id": "GPKLONFDEH_832", "locus": "GCA000006625_00809" }, { "type": "cds", "sequence": "contig_1", "start": 470711, "stop": 470848, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACACATGAAATTAATACTGAAAAACAAGGACATCAATCTAATAAAAATGTGCGTGAAATTGTAATAGATGCTTCATATAAATCAATATCATCACTAAATTTATCCCTTAAAACATTGATTATTTCAATATGA", "aa": "MATHEINTEKQGHQSNKNVREIVIDASYKSISSLNLSLKTLIISI", "aa_hexdigest": "819b92c20732005434e99cd6e68995ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5025.6916999999985, "isoelectric_point": 8.181376457214355 }, "id": "GPKLONFDEH_833", "locus": "GCA000006625_00810" }, { "type": "cds", "sequence": "contig_1", "start": 471224, "stop": 471319, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATTATAAAAGCACCCTTAATATGCCTACTACAGGTTTTGAAATGCGTGCTAATTTAAACGTTAAAGAACCAAAAATTCAACAATTTTGA", "aa": "MKDYKSTLNMPTTGFEMRANLNVKEPKIQQF", "aa_hexdigest": "e9edfaab35366b058394bbce546b5a53", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3661.233, "isoelectric_point": 9.398732948303223 }, "id": "GPKLONFDEH_834", "locus": "GCA000006625_00811" }, { "type": "cds", "sequence": "contig_1", "start": 471380, "stop": 471508, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGCATGATGGGCCGCCATATGCAAATGGAAATATTCATATTGGTCACGCTTTAAATAAAATACTAAAAGATTTTGTTGTTTCATATCACAATATGAATAATTATTACTCTCCTTATATTCCAGGATGA", "aa": "MHDGPPYANGNIHIGHALNKILKDFVVSYHNMNNYYSPYIPG", "aa_hexdigest": "a46f5e389b03704a67865bef39fd7e6b", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 42, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.2364553518158494e-15, "score": 52.69961166381836, "start": 1, "stop": 42 } ], "seq_stats": { "molecular_weight": 4773.323300000002, "isoelectric_point": 6.889363288879395 }, "id": "GPKLONFDEH_835", "locus": "GCA000006625_00812" }, { "type": "cds", "sequence": "contig_1", "start": 472151, "stop": 472420, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAGTATGTTAGTGCTAATGACGGAACGGGTTTAGTTCATAATGCGCCTGCTTTTGGTTTAGAAGACTATTATGCATGTAAAAAATATGGTATTGAAACAGTAGTTATGATAGACCAATTTGGTAAATACAATGCATTAGTAAATGATTTAGAATTAGAAAATATGTTTTATGAAGATGCTAATCAAGTTATTTTAAATCGTTTGATGAATGAACATTTATTAATCCATCATGAAGTTATCACTCATAGTGTTGCACATGATTGA", "aa": "MDEYVSANDGTGLVHNAPAFGLEDYYACKKYGIETVVMIDQFGKYNALVNDLELENMFYEDANQVILNRLMNEHLLIHHEVITHSVAHD", "aa_hexdigest": "a8fdf16c57351654cbe27d9395c240fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10172.281899999994, "isoelectric_point": 4.609892463684082 }, "id": "GPKLONFDEH_836", "locus": "GCA000006625_00813" }, { "type": "cds", "sequence": "contig_1", "start": 472439, "stop": 472582, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCGTGCCACAAAACAATGGTTTGTTTCTATTGAAAAAATTTTACCTAACATTTTGCAGACCCTAAATAACGATGTTAAATCAACTAGTTTTAGAGGAATTGAAAGAATGCATGAAATGATTGTTAATCGTAAGGAATGA", "aa": "MYRATKQWFVSIEKILPNILQTLNNDVKSTSFRGIERMHEMIVNRKE", "aa_hexdigest": "376766da85e16d5981cbb307ba0dce18", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5666.559299999999, "isoelectric_point": 9.815134239196777 }, "id": "GPKLONFDEH_837", "locus": "GCA000006625_00814" }, { "type": "cds", "sequence": "contig_1", "start": 472622, "stop": 472714, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGATGAGAATCATAATGCAATTATGGATCATGAATTAGTAGAAAATATCATTAATGTTTTAAATGAAAAAGGTGTTAATGCATGA", "aa": "MIFDENHNAIMDHELVENIINVLNEKGVNA", "aa_hexdigest": "266c18d9a7b233b9d2655185bf5e2e3e", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3436.8241000000007, "isoelectric_point": 4.346102714538575 }, "id": "GPKLONFDEH_838", "locus": "GCA000006625_00815" }, { "type": "cds", "sequence": "contig_1", "start": 472976, "stop": 473080, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGGTTCTAGATGGTGAAGGATATAAGATGTCAAAATCAAAAGGAAATGTTGTTGATCCATTAGATGTATGTAAGATTTATGGAGCAGATGTTTTAAGATTATGA", "aa": "MVLDGEGYKMSKSKGNVVDPLDVCKIYGADVLRL", "aa_hexdigest": "d98753bcee388e2b6d0da9490fd3d33f", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 34, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.2425543614128127e-12, "score": 42.81079864501953, "start": 1, "stop": 34 } ], "seq_stats": { "molecular_weight": 3714.333599999999, "isoelectric_point": 5.952997779846191 }, "id": "GPKLONFDEH_839", "locus": "GCA000006625_00816" }, { "type": "cds", "sequence": "contig_1", "start": 473930, "stop": 474460, "strand": "+", "frame": 2, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711", "SO:0001217", "UniRef:UniRef50_Q9PQ78", "UniRef:UniRef90_Q9PQ78" ], "nt": "ATGAATGATAATTATTTAATTTTAAGCATTGAATCAAGTTGTGATGAAACAAGTTTAGCGTTATTTGAAAATAATAAATTAATTGCTCATAAAATTAGTAGTTCAGCATCTATACAATCACTTCATGGCGGTGTTGTTCCTGAATTAGCAAGTCGTTATCATGAACAAAATATTAATCACCTATTTAATGAAATTTTAAATGAAACAAAAATCAACCCTTTAACTATCACTCACGTTGCTTACACTGCAATGCCTGGTTTGCCAGGTTGTTTACATGTAGGAAAAGTTTTTGCGAAACAATTAGCTGTTTTAATTAATGCTGAATTAGTGCCAATCAATCATTTACATGCGCATGTTTTTAGTGCAAGTATTAATCAAAATTTGACTTTTCCATTTTTGGGATTAGTGGTTTCAGGAGGGGAAAGTTGTATATATTTAGTAAATGATTATGATGAAATCAAAGTTTTAAATCAAACACACGATGATGCAATTGGTGAATGTTATGATAAAATTGCTCGTGTTCTTGGTTGA", "aa": "MNDNYLILSIESSCDETSLALFENNKLIAHKISSSASIQSLHGGVVPELASRYHEQNINHLFNEILNETKINPLTITHVAYTAMPGLPGCLHVGKVFAKQLAVLINAELVPINHLHAHVFSASINQNLTFPFLGLVVSGGESCIYLVNDYDEIKVLNQTHDDAIGECYDKIARVLG", "aa_hexdigest": "a58b58c071320208a689d5b98d05db4a", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 473930, "stop": 474763, "observations": { "mutation": [ 474458 ], "stop": [ 474458 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ78", "query_cov": 1.0, "subject_cov": 0.55, "identity": 1.0, "score": 353.0, "evalue": 4.89e-121, "gene_start": 1, "gene_end": 176, "reference_start": 1, "reference_end": 176, "reference_sequence": "MNDNYLILSIESSCDETSLALFENNKLIAHKISSSASIQSLHGGVVPELASRYHEQNINHLFNEILNETKINPLTITHVAYTAMPGLPGCLHVGKVFAKQLAVLINAELVPINHLHAHVFSASINQNLTFPFLGLVVSGGESCIYLVNDYDEIKVLNQTHDDAIGECYDKIARVLGWKYPGGPIIDKNYQENLATLEFIKSQPAAKDFSFSGLKTAVINYIHNAKQKKISFDPVVVASSFQKFAINEIIKKIKYYLNLYKLNHLAIGGGVSANSLLRKKIQSLDVISYIPEMIYTGDNAAMIGAYAYALIKNHKKSILIK" }, "paralog": false, "identity": 0.9963898916967509, "score": 548.0, "evalue": 1.19e-200, "description": "Internal stop codon at 474458. Nonsense mutation around 474458.", "psc": { "uniref90_id": "UniRef90_Q9PQ78", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q9PQ78", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ78", "EC:2.3.1.234", "UniRef:UniRef50_Q9PQ78", "COG:COG0533", "COG:J", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ78" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "GPKLONFDEH_840", "locus": "GCA000006625_00817" }, { "type": "cds", "sequence": "contig_1", "start": 474803, "stop": 474892, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATACAGGTGATAATGCTGCTATGATTGGGGCTTATGCTTATGCTTTAATTAAAAATCATAAGAAATCAATATTAATAAAATAA", "aa": "MIYTGDNAAMIGAYAYALIKNHKKSILIK", "aa_hexdigest": "efc1a6022e29d67b96240f954ef4ec30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3212.8245000000006, "isoelectric_point": 9.602904319763184 }, "id": "GPKLONFDEH_841", "locus": "GCA000006625_00818" }, { "type": "cds", "sequence": "contig_1", "start": 474892, "stop": 475128, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00438.25" ], "nt": "ATGGTAGAAAGAAAAGAATTTAGTATGAAATATAAAAAAATTATAACGAGCGAATCTGTTGGTGCAGGCCATCCAGATAAAATATGTGATCAGATTAGTGATGCAATTTTAGATGAATGCTTAGCACAAGATAAAAATTCACGTGTAGCTTGTGAAGTTTTAGCTTGTAATCGTTTAATTGTTATTGCTGGGGAAATTACCACGCATGCATATGTTGATGTAGTAAAAACCGCTTGA", "aa": "MVERKEFSMKYKKIITSESVGAGHPDKICDQISDAILDECLAQDKNSRVACEVLACNRLIVIAGEITTHAYVDVVKTA", "aa_hexdigest": "c838d2f0fb37490525d36f3ca0b3810f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0035AE390E", "query_cov": 0.8333333333333334, "subject_cov": 0.8441558441558441, "identity": 0.631, "score": 80.9, "evalue": 1.51e-18, "valid": false, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_A0A355EM24", "kegg_orthology_id": "K00789" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00438.25", "name": "S-AdoMet_synt_N", "length": 65, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 2.5348959544466443e-30, "score": 100.57001495361328, "start": 13, "stop": 77 } ], "seq_stats": { "molecular_weight": 8583.846499999996, "isoelectric_point": 5.574563789367674 }, "id": "GPKLONFDEH_842", "locus": "GCA000006625_00819" }, { "type": "cds", "sequence": "contig_1", "start": 475300, "stop": 475734, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02773.21", "PFAM:PF02772.21" ], "nt": "ATGCCATTAACAACAGTGTTAGCTCATGAATTACTAAAAGAAATCGAAAAACAAAGACGTAATAAAGAATTTGTTAAAATTCAAGCTGATATGAAATCACAAGTAAGTATTGATTATTCTAATTGTACGCCTGTTATTGAGACAATGTTGGTAAGTATTCAACATGATGAAGATTATAACATTGAATATTTTAATAAAAAAGTTAGCACAATTATGGATCAAATTGCTATAAAATATGGTTTAAATACCGATTTTAAAAAAATTATTAATTCCTCAGGGAGATTTGTAATTGGTGGTCCAATTGGTGACACTGGTTTAACTGGAAGAAAAATTATCGTAGATACTTATGGAGGAATAGGTCATCATGGTGGTGGTGCTTTTAGTGGCAAAGACCCTACTAAAGTTGATCGTAGTGCTTCATACTTTGCACGTTGA", "aa": "MPLTTVLAHELLKEIEKQRRNKEFVKIQADMKSQVSIDYSNCTPVIETMLVSIQHDEDYNIEYFNKKVSTIMDQIAIKYGLNTDFKKIINSSGRFVIGGPIGDTGLTGRKIIVDTYGGIGHHGGGAFSGKDPTKVDRSASYFAR", "aa_hexdigest": "97b25a522ab36d4664c191a65a84dd5b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02773.21", "name": "S-AdoMet_synt_C", "length": 48, "aa_cov": 0.3333333333333333, "hmm_cov": 1.0, "evalue": 4.984061710174283e-26, "score": 86.8976821899414, "start": 97, "stop": 144 }, { "id": "PF02772.21", "name": "S-AdoMet_synt_M", "length": 99, "aa_cov": 0.6597222222222222, "hmm_cov": 1.0, "evalue": 4.24213862249464e-19, "score": 64.4543228149414, "start": 1, "stop": 95 } ], "seq_stats": { "molecular_weight": 16030.131299999983, "isoelectric_point": 8.609317588806153 }, "id": "GPKLONFDEH_843", "locus": "GCA000006625_00820" }, { "type": "cds", "sequence": "contig_1", "start": 475816, "stop": 475935, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCAATGTTTGTTAATACATTCAAGACTAATTTGGTTGATGAAAAGAAAATTTTTGAAGCAATTAATAAAAGTTTTAATTTTGATATCAAAACGTTTATTAATGATTTAGAATTGTGA", "aa": "MAMFVNTFKTNLVDEKKIFEAINKSFNFDIKTFINDLEL", "aa_hexdigest": "114efbf6cc858f86d42246b54e502a16", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4630.3387999999995, "isoelectric_point": 4.992987251281736 }, "id": "GPKLONFDEH_844", "locus": "GCA000006625_00821" }, { "type": "tRNA", "sequence": "contig_1", "start": 476104, "stop": 476185, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 66.2, "nt": "GCTCAAGTGGCGAAATGGCAGACGCAGTTGACTCAAAATCAACCGAGAAATCATAAGGGTTCGAGTCCCTTCTTGAGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 476180, 476182 ], "id": "GPKLONFDEH_30", "locus": "GCA000006625_00822" }, { "type": "cds", "sequence": "contig_1", "start": 476249, "stop": 476764, "strand": "-", "frame": 3, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "COG:COG0266", "COG:L", "EC:3.2.2.23", "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078", "KEGG:K10563", "SO:0001217", "UniRef:UniRef50_Q9PQ76", "UniRef:UniRef90_Q9PQ76" ], "nt": "ATGCAATTAAGATATAATGATACGCGTCAATTTGGAACTTTTCACATTTATGAGCAACAATCTTTTTTAGATTCAAAAGAATTAAAAAAGATTGCTTTAGATCCGCTAGATAATAATTTTTCAGCGCAGTATTTGTATGAAAAATTAAAAAAAAGTAATAAAGCCATTAAAACAGCCTTGTTAGATCAATCTGTTGTTTCAGGAATTGGTAACATTTATGCTGATGAAATTTTATTTGCAGCTAAAATTTTTCCTACAATATTAGCTAAAAATCTTACTTTAAAAAATTATGAAAAGATCACAAAAGAAGCACAACGAATCTTATTATTATCAATTAAAAATAAAGGAACAACAATCCACACTTATAAATTTGGTAATGATGAAACAGGTTTGTTTCAAAAGATGTTATTAGTTCACACACACGCTAAAGAACCATGCCAAATTTGTGGAACAATTATTCAAAAAACAAAGGTCAACGGTCGGGGAACTTATTATTGTCCAAATTGTCAAAACTAA", "aa": "MQLRYNDTRQFGTFHIYEQQSFLDSKELKKIALDPLDNNFSAQYLYEKLKKSNKAIKTALLDQSVVSGIGNIYADEILFAAKIFPTILAKNLTLKNYEKITKEAQRILLLSIKNKGTTIHTYKFGNDETGLFQKMLLVHTHAKEPCQICGTIIQKTKVNGRGTYYCPNCQN", "aa_hexdigest": "51d8ee29228632c88ad19a7c586fe634", "start_type": "ATG", "rbs_motif": null, "genes": [ "mutM" ], "truncated": "both", "pseudogene": { "start": 476243, "stop": 477064, "observations": { "mutation": [ 476251, 476812 ], "stop": [ 476251, 476812 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ76", "query_cov": 1.0, "subject_cov": 0.6173285198555957, "identity": 0.953, "score": 327.0, "evalue": 1.44e-111, "gene_start": 1, "gene_end": 171, "reference_start": 105, "reference_end": 275, "reference_sequence": "MPELPEVQTIVDYLNHHVLDIFIKKTIVHLPKILKNKTAQEFENLLINHKIVKIERLGKYLLFFLSNNLVLCVHLRMEGKFYYQAKDEWFNLAHTHIIIEFNNGMQLRYNDTRQFGTFHIYEQESFLDSKELKKIALDPLDNNFSAQYLYGKLKKSNKAIKTALLDQSNVSGIGNIYADEILFAAKIFPTILAKNLTLKDYEKIAKEARRILLLSIENKGTTIHTYKFVNDETGLFQKMLLVHTHAKEPCQICGTIIQKTKVNGRGTYYCPNCQNQN" }, "paralog": false, "identity": 0.945054945054945, "score": 518.0, "evalue": 3.07e-189, "description": "Internal stop codon at 476251, 476812. Nonsense mutation around 476251, 476812.", "psc": { "uniref90_id": "UniRef90_Q9PQ76", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23" ], "uniref50_id": "UniRef50_Q9PQ76", "cog_id": "COG0266", "cog_category": "L", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ76", "EC:3.2.2.23", "UniRef:UniRef50_Q9PQ76", "COG:COG0266", "COG:L", "KEGG:K10563", "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ76" ], "product": "Formamidopyrimidine-DNA glycosylase" } }, "id": "GPKLONFDEH_845", "locus": "GCA000006625_00823" }, { "type": "cds", "sequence": "contig_1", "start": 476810, "stop": 477076, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01149.29" ], "nt": "ATGCCAGAGCTACCAGAAGTACAAACGATTGTTGATTATTTAAATCATCATGTTCTCGATATTTTTATTAAAAAAACTATTGTTCATTTACCTAAAATTTTAAAAAATAAAACACCACAAGAATTTGAAAAACTGTTAATTAATCATAAAATAGTCAAAATTAAACGTCTTGGTAAATATTTATTATTTTTTTTAAGTAATAATTTAGTATTAAGTGTACATTTGCGGATGGAGGGAAAATTTTATTATCAAGCAAAAGAGGAATGA", "aa": "MPELPEVQTIVDYLNHHVLDIFIKKTIVHLPKILKNKTPQEFEKLLINHKIVKIKRLGKYLLFFLSNNLVLSVHLRMEGKFYYQAKEE", "aa_hexdigest": "7156524dd7718dbd48a55d37088c74e6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01149.29", "name": "Fapy_DNA_glyco", "length": 87, "aa_cov": 0.9772727272727273, "hmm_cov": 1.0, "evalue": 2.290176671738811e-25, "score": 85.1330337524414, "start": 1, "stop": 86 } ], "seq_stats": { "molecular_weight": 10528.469799999993, "isoelectric_point": 9.522576713562014 }, "id": "GPKLONFDEH_846", "locus": "GCA000006625_00824" }, { "type": "cds", "sequence": "contig_1", "start": 477118, "stop": 477891, "strand": "-", "frame": 1, "gene": "polA", "product": "5'-3' exonuclease", "db_xrefs": [ "COG:COG0258", "COG:L", "EC:3.1.11.-", "GO:0003677", "GO:0008409", "GO:0017108", "GO:0033567", "SO:0001217", "UniRef:UniRef50_Q9PQ75", "UniRef:UniRef90_Q9PQ75" ], "nt": "ATGCCTACTAATGCCATTAAATTAGTAGCCTCAATGATTTTTAAGATTTTAAATCAAGAACAATTTACTTATGGTTTAATTGCTATGGACGCTTCTAAACAAACTTTTCGTGCTCAAGAATATGCTGCTTACAAAGCGACACGTAAACCTATGGATGCAAAACTAATTGTTCAATTACCCTATTTAAAAAAACTATTCGTTGCTATGGGTTTTCGAATAATTTCACAACCTGGAATTGAAGCTGACGATTTTGTTGGTTCTTTTAGTAATTTAATGAATAAGTTTGATGTTGATACAACTATTTATAGTTCAGATCGTGATATGTTGCAATTAATTAACAAAAACACAAAACTTAAACTCTTAAAAACAGGGACAAGTGTTATGCAAGAAATTAATTTAACAAATTTTTCATTATTAAATAATGGCATTTTACCGTATCAAATTATTGAATATAAGGGTTTAGTGGGTGATAGTAGTGATAATTTAATTGGCGTGAAGGGAATCGGCCCTAAAACAGCTATTAATTTACTTTTAAAATATGATAATATAGAAAATATTTATACTAATTTAGAAAAAATAGCACCTAGTGTTAAAAATAAATTAATTGAACACAAAGATATGGCTTTTTTATCAAAAAAATTAGCGGCGATTCAAACGGATTTATTGTTTGATGAAAAATTAGAAAATTTTGTTTTAAAACCATATAATACTCAAGAATTAGATGAGTTATTCACATCTTTAAAAATAAATAATATGCAAAATTATTATAAATAA", "aa": "MPTNAIKLVASMIFKILNQEQFTYGLIAMDASKQTFRAQEYAAYKATRKPMDAKLIVQLPYLKKLFVAMGFRIISQPGIEADDFVGSFSNLMNKFDVDTTIYSSDRDMLQLINKNTKLKLLKTGTSVMQEINLTNFSLLNNGILPYQIIEYKGLVGDSSDNLIGVKGIGPKTAINLLLKYDNIENIYTNLEKIAPSVKNKLIEHKDMAFLSKKLAAIQTDLLFDEKLENFVLKPYNTQELDELFTSLKINNMQNYYK", "aa_hexdigest": "d55ccb62a745d43abc2057cfec32771f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_Q9PQ75", "query_cov": 1.0, "subject_cov": 0.889273356401384, "identity": 1.0, "score": 498.0, "evalue": 1.7e-177, "valid": true, "gene": "polA", "product": "5'-3' exonuclease", "ec_ids": [ "3.1.11.-" ], "uniref50_id": "UniRef50_Q9PQ75", "cog_id": "COG0258", "cog_category": "L", "go_ids": [ "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ75" ], "product": "5'-3' exonuclease" }, "genes": [ "polA" ], "id": "GPKLONFDEH_847", "locus": "GCA000006625_00825" }, { "type": "cds", "sequence": "contig_1", "start": 477991, "stop": 478509, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATACGAGTCGGCACTTTTGATAGTTTTGGAATTAATCGTTTATTTTTATTAAATAATTTGAATGAAATTTTTGAAAAAACAGGATTGAATGGTCATTTTTTTGATTTAAATTTAGTGGGTTTAGATTATGCAAAAGATATGAGTGTTAATGATCGTTATTTAGATGATGAAATTCAGTATTTAGGAATTGATTTGAATAGTTTAAATTACGCAAATTATAAAACTGAAATTGATTATAATAAACTAAAATATACAATTAAAGACTTTTATGAAATAAACACAAATTATGAAACAAACATTCTTGCACAAGTTTTAAACATTAAGCAATCAAAAACTAAAAACGGGAATGATATCTTTTATTTAGAAACACTAATTGATAATAAAAAACAAACACTAACAATTTTTCAAAATAGTAAATATTTAATTGATGAAATTAGTATTGGAGGTATTTATGTTTTTGGAGTTAAATTATTAAATCATTTTAATTTTATTGTAAATATTAAGCAAAGGATTTAG", "aa": "MIRVGTFDSFGINRLFLLNNLNEIFEKTGLNGHFFDLNLVGLDYAKDMSVNDRYLDDEIQYLGIDLNSLNYANYKTEIDYNKLKYTIKDFYEINTNYETNILAQVLNIKQSKTKNGNDIFYLETLIDNKKQTLTIFQNSKYLIDEISIGGIYVFGVKLLNHFNFIVNIKQRI", "aa_hexdigest": "8ec47a197fdc19faae0158f3e29a79a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20156.713600000006, "isoelectric_point": 5.382845878601075 }, "id": "GPKLONFDEH_848", "locus": "GCA000006625_00826" }, { "type": "cds", "sequence": "contig_1", "start": 478843, "stop": 479085, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "GTGATTGAATTAGTTAAAACTATTGCAAATTTTGATATTGCTACTTCCGATAATTTTCGCCGTGCAATATCAAAAAAAGATGAAAAAATTTTAATCCAATTAAAAGATGATTTTATCAATGGTGCTTTGAATAATAATTATAAACAACCATTGGTTAATCAAATTTTTGAATATATTTTTTCGTTTGCACATTATGGTTTTAACCATTCACATTCACTAGCATATTCATATATTAGTTATTGA", "aa": "MIELVKTIANFDIATSDNFRRAISKKDEKILIQLKDDFINGALNNNYKQPLVNQIFEYIFSFAHYGFNHSHSLAYSYISY", "aa_hexdigest": "def5f52f07645ad8ece49ffa233e6fdc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 45, "aa_cov": 0.5625, "hmm_cov": 1.0, "evalue": 4.90293010140438e-09, "score": 31.325239181518555, "start": 2, "stop": 46 } ], "seq_stats": { "molecular_weight": 9369.495299999999, "isoelectric_point": 6.816268348693847 }, "id": "GPKLONFDEH_849", "locus": "GCA000006625_00827" }, { "type": "cds", "sequence": "contig_1", "start": 479146, "stop": 480621, "strand": "-", "frame": 1, "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "COG:COG0587", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0008408", "SO:0001217", "UniRef:UniRef50_Q9PQ74", "UniRef:UniRef90_Q9PQ74" ], "nt": "ATGACAAGTAAAGAATTTGAAATTCAAGATGATTTTGATGACTTAATTATTGTTTGTAAAAAAGGAACTATAGTTTTTAAGAGTCCTAATTTTTATCAAACGCATGATCAAAATGCTCCTAATGCGATTGCATTACAAAGTGTTTTCTATGCTAACAAAAATGATAAAATTGTTTTTTTAGCAATGTTAGCAATCAAAAATGATTTAAAGTTAGAAGATTTTAAAAATTGTTGCGATTTTGATAATAATCATTTTCTAAATGATAATCTAGCACAATCATTTTTTTCACCAATTGCTTTAAATAATTTAAATAAAGTTTTAAATGAATTAAAAGTACAAATTCATGATTTACCAATTAATATTCCGGTTTATGATAAACAAAATTCAATTATTTCAAGTGAAATTTTAAAACAATTATGTATTAGCGGATTAAAAAAACGCTTAAATGCTAACGATGGCCAAGTTAATAAAATTTATGTTCAAAGATTAAAATATGAATTAGATGTTATTAATGAAAAACAATTTGATGATTATTTTTTAATTGTTTATGATTTTATTAATTTTGCTAAAAGTAATGGTATCATTGTAGGGCCAGGACGTGGGAGTGCTGCTGGTTCGTTAGTAGCTTATTGTTTACATATTACAGATATTGATCCTATTAAACATAATTTAATTTTTGAACGTTTTTTAAATCCTACCAGAAAAAGTATGCCAGATATTGATACTGATATTATGGATGAAAAACGCGATCTAGTTATTGAATATTTATTTGAAAAATATGGCAATGATCATGTTGCTTACATTTTAACGTTTCAACGTTTAAAAGCGAAGATGGCTATTCGAGATGTAGGGCGGATTTTGGGAATTGATTTAAAAGTAATTGATAAAATTTGTAAAAATATTAAACCTGAATATGATGAAGATCTTGATTTAGCAATTAAAAAAAATACGATTTTAAAAGAAATGTATCTTTTGCATAAAGAGTTGTTTGAAATTTCTAAAAAATTAATTAATGCTCCTCGCCAAATAGGCACACACGCTGCAGGGATAATTTTAAGTGATAGTTTAATTTCAAATATTATTCCAATTCAACTAGGTATTAATGACCGTCCTTTATCACAATATTCAATGGAATATTTAGAGCGTTTTGGTTTAATTAAAATGGATTTATTAGGTTTGAAAAATTTAACAATTATTGATAATGTTTTAAAAATGATTTATGAAAATCAAAATAAAAAAATTGATCTCTTTAACATTAACTATAATGATAAATTTGTCTTTGAAGATTTAGCTAAAGCAAGGACAAACGGAATATTTCAACTAGAATCGCCCGGAATGAAAAAAGTACTATTAAAGGTCAAACCTCAGAATATTGAAGATATTTCTATTGTTTCAGCTCTTTTTCGTCCTGGTCCACAACAAAATATTAAAACATTTGTTGAACGTCGTTTTAAACGTGAAGAGTTTAGTTATTGA", "aa": "MTSKEFEIQDDFDDLIIVCKKGTIVFKSPNFYQTHDQNAPNAIALQSVFYANKNDKIVFLAMLAIKNDLKLEDFKNCCDFDNNHFLNDNLAQSFFSPIALNNLNKVLNELKVQIHDLPINIPVYDKQNSIISSEILKQLCISGLKKRLNANDGQVNKIYVQRLKYELDVINEKQFDDYFLIVYDFINFAKSNGIIVGPGRGSAAGSLVAYCLHITDIDPIKHNLIFERFLNPTRKSMPDIDTDIMDEKRDLVIEYLFEKYGNDHVAYILTFQRLKAKMAIRDVGRILGIDLKVIDKICKNIKPEYDEDLDLAIKKNTILKEMYLLHKELFEISKKLINAPRQIGTHAAGIILSDSLISNIIPIQLGINDRPLSQYSMEYLERFGLIKMDLLGLKNLTIIDNVLKMIYENQNKKIDLFNINYNDKFVFEDLAKARTNGIFQLESPGMKKVLLKVKPQNIEDISIVSALFRPGPQQNIKTFVERRFKREEFSY", "aa_hexdigest": "452ecd69a33f5fe94cf8030b56c9892b", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaE" ], "truncated": "both", "pseudogene": { "start": 478843, "stop": 480900, "observations": { "mutation": [ 479148, 480627, 480636 ], "stop": [ 479148, 480627, 480636 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ74", "query_cov": 1.0, "subject_cov": 0.5067079463364293, "identity": 1.0, "score": 968.0, "evalue": 0.0, "gene_start": 1, "gene_end": 491, "reference_start": 94, "reference_end": 584, "reference_sequence": "MFINLNVHSHYSLLNSTLSIDDLIKYALDNKQPYVCLTDLNNMYGCIEFYDKAKAHNLTPIIGLEFEYQNATLVAYAKNYNGYLKLIKWSSWIMTSKEFEIQDDFDDLIIVCKKGTIVFKSPNFYQTHDQNAPNAIALQSVFYANKNDKIVFLAMLAIKNDLKLEDFKNCCDFDNNHFLNDNLAQSFFSPIALNNLNKVLNELKVQIHDLPINIPVYDKQNSIISSEILKQLCISGLKKRLNANDGQVNKIYVQRLKYELDVINEKQFDDYFLIVYDFINFAKSNGIIVGPGRGSAAGSLVAYCLHITDIDPIKHNLIFERFLNPTRKSMPDIDTDIMDEKRDLVIEYLFEKYGNDHVAYILTFQRLKAKMAIRDVGRILGIDLKVIDKICKNIKPEYDEDLDLAIKKNTILKEMYLLHKELFEISKKLINAPRQIGTHAAGIILSDSLISNIIPIQLGINDRPLSQYSMEYLERFGLIKMDLLGLKNLTIIDNVLKMIYENQNKKIDLFNINYNDKFVFEDLAKARTNGIFQLESPGMKKVLLKVKPQNIEDISIVSALFRPGPQQNIKTFVERRFKREEFSYWNEATRKILEPTYGIIVYQEQVIELVKTIANFDIATSDNFRRAISKKDEKILIQLKDDFINGALNNNYKQPLVNQIFEYIFSFAHYGFNHSHSLAYSYISYWLAYLKHYYTLEFLSVLLSHTSASKDKLLSYLNEAKEFNISIKGPDIQYFSNDFVIDTQKQIIRFGFKTIKGFGDELLKKIKSALQKSTLSDYISYIDALKKGNVSLKNIEILIRVGTFDSFGINRLFLLNNLNEIFEKTGLNGHFFDLNLVGLDYAKDMSVNDRYLDDEIQYLGIDLNSLNYANYKTEIDYNKLKYTIKDFYEINTNYETNILAQVLNIKQSKTKNGNDIFYLETLIDNKKQTLTIFQNSKYLIDEISIGGIYVFGVKLLNHFNFIVNIKQRI" }, "paralog": false, "identity": 0.9956204379562044, "score": 1353.0, "evalue": 0.0, "description": "Internal stop codon at 479148, 480627, 480636. Nonsense mutation around 479148, 480627, 480636.", "psc": { "uniref90_id": "UniRef90_Q9PQ74", "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q9PQ74", "cog_id": "COG0587", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ74", "EC:2.7.7.7", "UniRef:UniRef50_Q9PQ74", "COG:COG0587", "COG:L", "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ74", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ74" ], "product": "DNA polymerase III subunit alpha" } }, "id": "GPKLONFDEH_850", "locus": "GCA000006625_00828" }, { "type": "cds", "sequence": "contig_1", "start": 480634, "stop": 480900, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAATTTAAATGTGCATTCACATTATTCTTTACTTAATAGTACTTTAAGTATTGATGATTTAATTAAATACGCATTAGATAACAAACAACCATACGTTTGCTTAACTGATTTAAATAATATGTATGGCTGTATCGAATTTTATGATAAAGCAAAAGCACATAATTTAACTCCTATTATCGGATTAGAATTTGAATATCAAAATGCTACTTTAGTAGCTTATGCTAAAAATTATAATGGCTATTTAAAATTAATCAAATGA", "aa": "MFINLNVHSHYSLLNSTLSIDDLIKYALDNKQPYVCLTDLNNMYGCIEFYDKAKAHNLTPIIGLEFEYQNATLVAYAKNYNGYLKLIK", "aa_hexdigest": "7af14f2924425a8c85c716e7c2011026", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10170.582199999995, "isoelectric_point": 6.2359422683715815 }, "id": "GPKLONFDEH_851", "locus": "GCA000006625_00829" }, { "type": "cds", "sequence": "contig_1", "start": 480900, "stop": 481112, "strand": "-", "frame": 2, "gene": null, "product": "putative protein UU416", "db_xrefs": [ "RefSeq:WP_006688799.1", "SO:0001217", "UniParc:UPI0000139799", "UniRef:UniRef100_Q9PQ73", "UniRef:UniRef50_Q9PQ73", "UniRef:UniRef90_Q9PQ73" ], "nt": "ATGAGTGATAAAAAAAGTGATTTTGAAAAGTTATTTGAGCGTTCAAAAGTACAAGAAACATCTATTTTCGATATTAATGATATTGATGAACAAAAACCGTACGAAGAACATTCGATTGAAGAATTATCATATATCTTAGAATATGAAAAAATTGATAAGAAAACACGTGAAAAGATCAAAAAAATTATTAAAGAGAAACAACAAAATTTATAA", "aa": "MSDKKSDFEKLFERSKVQETSIFDINDIDEQKPYEEHSIEELSYILEYEKIDKKTREKIKKIIKEKQQNL", "aa_hexdigest": "e4bbf817419d2e96c3bdfe34fbf7e781", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0000139799", "ncbi_nrp_id": "WP_006688799.1", "uniref100_id": "UniRef100_Q9PQ73", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139799", "RefSeq:WP_006688799.1", "UniRef:UniRef100_Q9PQ73" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ73", "uniref90_id": "UniRef90_Q9PQ73" }, "psc": { "uniref90_id": "UniRef90_Q9PQ73", "product": "Uncharacterized protein UU416", "uniref50_id": "UniRef50_Q9PQ73" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ73" ], "product": "Uncharacterized protein UU416" }, "genes": [], "id": "GPKLONFDEH_852", "locus": "GCA000006625_00830" }, { "type": "cds", "sequence": "contig_1", "start": 481197, "stop": 481328, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02272.25" ], "nt": "TTGGTTGCTAGTCAATTTAATGGAGGCGGACATGATTTGGCAGCAGGTTGTAGTTTAACTAATATAGATCAATTAGAAAATTTATTAAGTGCTCTTGATGCACTAATTAAAAATAAAATAGTTGTTGATTAA", "aa": "MVASQFNGGGHDLAAGCSLTNIDQLENLLSALDALIKNKIVVD", "aa_hexdigest": "74e03cff854949803a508fe35bf5c2d5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02272.25", "name": "DHHA1", "length": 42, "aa_cov": 0.8372093023255814, "hmm_cov": 1.0, "evalue": 5.918240396430348e-09, "score": 31.958206176757812, "start": 3, "stop": 38 } ], "seq_stats": { "molecular_weight": 4470.0438, "isoelectric_point": 4.504115104675294 }, "id": "GPKLONFDEH_853", "locus": "GCA000006625_00831" }, { "type": "cds", "sequence": "contig_1", "start": 481620, "stop": 482162, "strand": "-", "frame": 2, "gene": "nrnA", "product": "Bifunctional oligoribonuclease/PAP phosphatase NrnA", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.13.3", "EC:3.1.3.7", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_A0A084EYE9", "UniRef:UniRef90_A0AAC9T261" ], "nt": "ATGCAAAAAGATTTGTTAACAAAATTAATTGAACAAACATATGGTTTTAATAAAATCACTATTTTTGTTCATACAAATCCTGATTGTGATGCTTTAGGTTCTGCTTTCGCATTAGCACGAATTTTAAAATTAAATACTTTTGGAACAAGAGTTAAAATTGTTGGGGTTAACGCATTAAATCCTAATGATTTTAAAAATTTTTTTACATTTGATCCCAATGAAGTTGATGATGAATTTATTAAAGATTCAATAGCTTTTATTGTTGATACAGCTAATCAAGAAAGAGTTTTATCACAAAAACATAGATTAGCAAAAAAAACAATTTTAGTTGATCATCATGTAAAAACAATAACTTATACTGATCTTGAATACATTAATGATCAATCAATTGCAGCTTGTGAAATGATAGCATATTCATTAATGCATACTAATTTAAATTTTGATTTAAAAACTTTAAATTATCTTTTATTAGGTATTACTACTGATTCAAATCGTTTGATGTACGATAAAGTAAGTGATTTTACATATGAGATTATGTCTTGA", "aa": "MQKDLLTKLIEQTYGFNKITIFVHTNPDCDALGSAFALARILKLNTFGTRVKIVGVNALNPNDFKNFFTFDPNEVDDEFIKDSIAFIVDTANQERVLSQKHRLAKKTILVDHHVKTITYTDLEYINDQSIAACEMIAYSLMHTNLNFDLKTLNYLLLGITTDSNRLMYDKVSDFTYEIMS", "aa_hexdigest": "0e76b0b633066f5db39864654bb2ecd2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "nrnA" ], "truncated": "5-prime", "pseudogene": { "start": 481317, "stop": 482162, "observations": { "mutation": [ 481403, 481451, 481478, 481622 ], "stop": [ 481403, 481451, 481478, 481622 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T261", "query_cov": 1.0, "subject_cov": 0.5607476635514018, "identity": 1.0, "score": 357.0, "evalue": 8.91e-123, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MQKDLLTKLIEQTYGFNKITIFVHTNPDCDALGSAFALARILKLNTFGTRVKIVGVNALNPNDFKNFFTFDPNEVDDEFIKDSIAFIVDTANQERVLSQKHRLAKKTILVDHHVKTITYTDLEYINDQSIAACEMIAYSLMHTNLNFDLKTLNYLLLGITTDSNRLMYDKVSDFTYEIMSWFFKNNVKHYQIYQQLYERNLNDILFDNELVKTIKTQGQVAYLNIDPQWNQKYHFTRWGDKVYLLSNIKNYPIWFIVYFDENTNTYKVSLRSNKYKVRLVASQFNGGGHDLAAGCSLTNIDQLENLLSALDALIKNKIVVD" }, "paralog": false, "identity": 0.9857651245551602, "score": 551.0, "evalue": 8.76e-202, "description": "Internal stop codon at 481403, 481451, 481478, 481622. Nonsense mutation around 481403, 481451, 481478, 481622.", "psc": { "uniref90_id": "UniRef90_A0AAC9T261", "gene": "NrnA", "product": "Bifunctional oligoribonuclease/PAP phosphatase NrnA", "ec_ids": [ "3.1.13.3", "3.1.3.7" ], "uniref50_id": "UniRef50_A0A084EYE9", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T261", "EC:3.1.13.3", "EC:3.1.3.7", "UniRef:UniRef50_A0A084EYE9", "COG:COG0618", "COG:FT", "KEGG:K06881" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084EYE9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EYE9" ], "product": "Phosphoesterase DHH family protein" } }, "id": "GPKLONFDEH_854", "locus": "GCA000006625_00832" }, { "type": "cds", "sequence": "contig_1", "start": 482180, "stop": 483352, "strand": "-", "frame": 3, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "EC:2.8.1.4", "GO:0000049", "GO:0002937", "GO:0004810", "GO:0005524", "GO:0005829", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0140741", "RefSeq:WP_006689025.1", "SO:0001217", "UniParc:UPI0000136E2C", "UniRef:UniRef100_Q9PQ71", "UniRef:UniRef50_Q9PQ71", "UniRef:UniRef90_Q9PQ71" ], "nt": "ATGAAACCAATAATTTATATTAAATATGGGGAATTAACACTAAAAGGTAAAAATCGAACACAATTTATAAAAGTTTTAGTGCACAATATCAAACAAATATTAATACAATATCAAAATTTAACATATTGTGTTGGTTATGATAATTTGAAAATTATCAATTTAGAAAATTATAATTTAAAACAAATTATTGATGATTTGAAAGAAGTTTATGGAATTGCTTTTATTTGTATTGCTTATCAAGTTGATAAAGAACTTAGTGAAATTCAATTAGCTTGTAAAGAATTTATAAATGATTATGAACAAACATTTAAAATTGAAGCCCGTCGTAGTGATAAAAGTTTTAAATACAATTCAATGGAAATTAAACAAATGTGCGCTGCTTATTTATTGCAAAATTCACCCCTTTTAAAAGTTGATGTTCATCGTCCACAACTATTAATTAATATTGAAATAAAATATGATTGTGCTATAGTTTATGGTCATAAAATTATGGGTGCTAAAGGATTGCCGGTAGGTATTAATGGTAAGGCCTTAGTGTTACTGTCTGGAGGAATCGATTCTCCTGTTGCTTCTCGTTTAATTATGAAACGAGGAATTAGTATTGATTTTATTACATTTATTACTCCGCCGCACACTTCACAAAAAGCTTTAGATAAAACAATTGCATTAGCAAAACAAATAACATTAGATAATAAATTAACAAAAGCGAATCTATATGTATGCAATTTCACTAAATTACAAGACGAAATTGCGCACATTTCTAAAGAATCTTATCGAATTACACTTATGCGTCGTTATTTTATGCGTATTGCTAAACGTTTAGCAATAAGTGTTAAAGCAAATGCTTTAGTAACTGGGGAGGCACTTGGACAAGTTGCTTCACAAACTTTAAATAGCATGCAAACAATTAGTAGTGTTTTAGATAATTTTTTAGTTTTAAGACCTTTAATAGCTTACGATAAAGAAGAAATAATTAGTTTAGCTAAACGGTTTAATACTTATGAATTATCGATTTTGCCATACGATGATTCATGTAGCTTGTTTGCACCTAAGAATCCAACAACCAATCCTAATGTTCAAACAGCTGCTAAATTAGAAGAAGAATCATTAGTTTTAGATGCAATTTATGAATTAGTTTACACTAAAGAAATCACTAAAATTAATTTATCATAA", "aa": "MKPIIYIKYGELTLKGKNRTQFIKVLVHNIKQILIQYQNLTYCVGYDNLKIINLENYNLKQIIDDLKEVYGIAFICIAYQVDKELSEIQLACKEFINDYEQTFKIEARRSDKSFKYNSMEIKQMCAAYLLQNSPLLKVDVHRPQLLINIEIKYDCAIVYGHKIMGAKGLPVGINGKALVLLSGGIDSPVASRLIMKRGISIDFITFITPPHTSQKALDKTIALAKQITLDNKLTKANLYVCNFTKLQDEIAHISKESYRITLMRRYFMRIAKRLAISVKANALVTGEALGQVASQTLNSMQTISSVLDNFLVLRPLIAYDKEEIISLAKRFNTYELSILPYDDSCSLFAPKNPTTNPNVQTAAKLEEESLVLDAIYELVYTKEITKINLS", "aa_hexdigest": "4760ece8243c5529d26caf370c15792b", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "ups": { "uniparc_id": "UPI0000136E2C", "ncbi_nrp_id": "WP_006689025.1", "uniref100_id": "UniRef100_Q9PQ71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000136E2C", "RefSeq:WP_006689025.1", "UniRef:UniRef100_Q9PQ71" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ71", "uniref90_id": "UniRef90_Q9PQ71" }, "psc": { "uniref90_id": "UniRef90_Q9PQ71", "gene": "thiI", "product": "Probable tRNA sulfurtransferase", "ec_ids": [ "2.8.1.4" ], "uniref50_id": "UniRef50_Q9PQ71", "cog_id": "COG0301", "cog_category": "HJ", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0004810", "GO:0000049", "GO:0140741", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0002937" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ71" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [ "thiI" ], "id": "GPKLONFDEH_855", "locus": "GCA000006625_00833" }, { "type": "cds", "sequence": "contig_1", "start": 483435, "stop": 483608, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTGTTATTTAAGAAAAATTAGTAAAGGAATTTTTGCTATTGCTTTTTTAACAAGTGAAGATTATATTAATCCGCAAGCATTTGATATTAATAAAAATGAAATTATTTTAGTTAATTATGAGCTTGATCTAAATGAATTCGAAAAAGTTATTTCAAAAGCAAAAACATGA", "aa": "MYCYLRKISKGIFAIAFLTSEDYINPQAFDINKNEIILVNYELDLNEFEKVISKAKT", "aa_hexdigest": "a39789d342463eec686bcfb4b66e42fc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6679.6646999999975, "isoelectric_point": 5.179817008972167 }, "id": "GPKLONFDEH_856", "locus": "GCA000006625_00834" }, { "type": "cds", "sequence": "contig_1", "start": 483666, "stop": 484022, "strand": "-", "frame": 2, "gene": null, "product": "MPN551 family DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C5RMD4", "UniRef:UniRef90_A0A2C9DZ60" ], "nt": "ATGGTTAAAATTTATAATGAACCAATCGATCCAATATACGCTCAAGCCGGTGTTGTAATTGAACCAAAAATCAAAGATTATGTTGAGACTGTTTTAAAAGTTAAATATAAACAATACGAACCTGCGCAAATTGGTTATGATGTGTTTAAAGATAATCAAATTTTTGGTGGAATTCCGGATGGTGAACCAATTGATGAAAATGGTCAATTATTGTACCCAAATCAACCAATGTTAGAAATTAAAACAACATCTATTGATAGTTTTTCATTTAAAACAATTGATTATGTTCTTGTGCTACAAAAAGATGAACATAATCATCCAATTATAAAAAAGAAAGGCGATAAACGTGCAAAATGA", "aa": "MVKIYNEPIDPIYAQAGVVIEPKIKDYVETVLKVKYKQYEPAQIGYDVFKDNQIFGGIPDGEPIDENGQLLYPNQPMLEIKTTSIDSFSFKTIDYVLVLQKDEHNHPIIKKKGDKRAK", "aa_hexdigest": "92bc89bd88d61187953e66aed1ecbabe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 483363, "stop": 484196, "observations": { "mutation": [ 483377, 483437, 483668, 484031 ], "stop": [ 483377, 483437, 483668, 484031 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DZ60", "query_cov": 1.0, "subject_cov": 0.4097222222222222, "identity": 1.0, "score": 241.0, "evalue": 1.87e-78, "gene_start": 1, "gene_end": 118, "reference_start": 59, "reference_end": 176, "reference_sequence": "MPFIKTPNKDFGLVDNQIVLSSDYKKRYQRYFAKITGSRLGSILNIGDFTNPVRTWAMMVKIYNEPIDPIYAQAGVVIEPKIKDYVETVLKVKYKQYEPAQIGYDVFKDNQIFGGIPDGEPIDENGQLLYPNQPMLEIKTTSIDSFSFKTIDYVLVLQKDEHNHPIIKKKGDKRAKWFNNDESEVIISDEYQLQLGLYCYLRKISKGIFAIAFLTSEDYINPQAFDINKNEIILVNYELDLNEFEKVISKAKTWYEEYIIKGISPKLTKEDLEWYKVWINKYETNNLY" }, "paralog": false, "identity": 0.9855595667870036, "score": 542.0, "evalue": 1.03e-199, "description": "Internal stop codon at 483377, 483437, 483668, 484031. Nonsense mutation around 483377, 483437, 483668, 484031.", "psc": { "uniref90_id": "UniRef90_A0A2C9DZ60", "product": "MPN551 family DNA-binding protein", "uniref50_id": "UniRef50_A0A0C5RMD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DZ60", "UniRef:UniRef50_A0A0C5RMD4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0C5RMD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C5RMD4" ], "product": "YqaJ viral recombinase domain-containing protein" } }, "id": "GPKLONFDEH_857", "locus": "GCA000006625_00835" }, { "type": "cds", "sequence": "contig_1", "start": 484029, "stop": 484196, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTATTAAAACGCCAAATAAAGATTTTGGATTAGTTGATAACCAAATTGTTTTATCAAGCGATTATAAAAAACGTTATCAACGATATTTTGCAAAAATTACAGGTAGTAGATTAGGTTCTATTTTGAATATTGGTGATTTTACTAATCCAGTTCGTACCTGA", "aa": "MPFIKTPNKDFGLVDNQIVLSSDYKKRYQRYFAKITGSRLGSILNIGDFTNPVRT", "aa_hexdigest": "1a138d4cea5d34607d8eb1f145e5fde3", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6325.2137, "isoelectric_point": 9.991777610778808 }, "id": "GPKLONFDEH_858", "locus": "GCA000006625_00836" }, { "type": "cds", "sequence": "contig_1", "start": 484311, "stop": 484823, "strand": "+", "frame": 3, "gene": null, "product": "DUF3137 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0Q2", "UniRef:UniRef90_A0AAC9T0Q2" ], "nt": "ATGAAAACAATAAATTTAAAAAAAGAATTTAATCAATATTGTATTAGTTTAAAAACTCAAGATCTTATTAAACAATACTTTAATGATGAAAAATTAAAAGAAAAATTATTAAAAAATCGTTTAATTGGATTAATTAGTTTAATTATCATTTTTATAATTGGTGTGGGAATTTTAATTCTTGGTGCTTTTATTAATAAAGGTAATGGCGCTAATTTAAATAATGGGGCAATAACTTGTGTTATCATAGGAATTTTCTTTTTGTTATGTTTATTAATTCCTTACACTATTGTTGCAAATCAAAATAAAAAGATTATGCATAAATATATTGAATATCATTTTAATGAAGTTAATGTGGGACAACTAATTAAAAAAATAAGTACTTTTTTTCAAGAATTTAGGTTATTAACAGAAGGTTTTTTAAATTTTAAACAAAGTAATCAAGTTATTGATATCAATTATTGTAATGAAAAATATAGTTTAATTTATTCATTCCAACAAAAACCACAAGTATGA", "aa": "MKTINLKKEFNQYCISLKTQDLIKQYFNDEKLKEKLLKNRLIGLISLIIIFIIGVGILILGAFINKGNGANLNNGAITCVIIGIFFLLCLLIPYTIVANQNKKIMHKYIEYHFNEVNVGQLIKKISTFFQEFRLLTEGFLNFKQSNQVIDINYCNEKYSLIYSFQQKPQV", "aa_hexdigest": "a74a9b0959cc10c062eb31e86e2d089d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 484311, "stop": 485057, "observations": { "mutation": [ 484821, 484866, 484914, 485019 ], "stop": [ 484821, 484866, 484914, 485019 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0Q2", "query_cov": 1.0, "subject_cov": 0.6854838709677419, "identity": 1.0, "score": 327.0, "evalue": 5.1e-112, "gene_start": 1, "gene_end": 170, "reference_start": 1, "reference_end": 170, "reference_sequence": "MKTINLKKEFNQYCISLKTQDLIKQYFNDEKLKEKLLKNRLIGLISLIIIFIIGVGILILGAFINKGNGANLNNGAITCVIIGIFFLLCLLIPYTIVANQNKKIMHKYIEYHFNEVNVGQLIKKISTFFQEFRLLTEGFLNFKQSNQVIDINYCNEKYSLIYSFQQKPQVWLIKSTNGNKISSKEWDLKGNYDLKIYELEKWNAGFSKFYLENNIESIAFQIESKLSTIINDFEELWIKGGGFLNEEY" }, "paralog": false, "identity": 0.9838709677419355, "score": 478.0, "evalue": 6.96e-174, "description": "Internal stop codon at 484821, 484866, 484914, 485019. Nonsense mutation around 484821, 484866, 484914, 485019.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0Q2", "product": "DUF3137 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0Q2", "UniRef:UniRef50_A0AAC9T0Q2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0Q2" ], "product": "DUF3137 domain-containing protein" } }, "id": "GPKLONFDEH_859", "locus": "GCA000006625_00837" }, { "type": "cds", "sequence": "contig_1", "start": 485044, "stop": 485886, "strand": "+", "frame": 1, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "GO:0045892", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_Q9PQ68", "UniRef:UniRef90_Q9PQ68" ], "nt": "ATGAAGAATACTAAAACATCGCAAGAACAAGTAATAGAATTAAAATTAAGTGATCGGCAAAAAAAAGTATTAAAATCTATCGTCGATGAATACACTATTAGTGCAACACCGGTCTCTTCAAAACTTTTAGTACAAAAAGAATTCAAAGATCAATCTTCTGCTACAATTCGTAACGAAATGATGTTTCTTGAAAAAAACGGATTAATTGAAAAACAGCACATTAGTGGTGGGCGTGTTCCATCACTAAAAGGTTATGATTTTTATAATAAAAATTTAATTAATAAAAATAATAATGTTTCTGATAATTTTAAAATGCGTTTACATAAAATCCTTAGTAAACGATATTCCAATATTGATGAAATTTTAAATGCTGCTGTAAGTATCATTAATGAAACAACCCAATTACCAGCTGTTGTTACAAAAACAAGTAGTGATGAATTATTAAAACGAATTGATTTAGTAAAAATCAATGATAATTCGGCGTTAGTGTTAATTGTAACTTCAAGCGAAAACATTTTAACTCATTCAATTAAGCTTGATAAAAACACAAAATTTAATGATTTACAAACGTGTTTTAGTGTTTTAGATGAACGATTAGTTGATACTAAACTTAGTTTAATCAGTTCTAAATTAGATTTAATTGTCGATATAGTTCGTAATAAACTTGAAGAAGTAGAATACTATTTTAGTAAAATTGTACATCGTGTTTTTGATTTTTACGCTTATAAACCTAACATTGATTCTAAAACTTATGGAGTTAAATACTTAACTAAACATTCTGAATTTGAAGATCAACAAAAACTAAATGATTTACTAAATTTATTAGAAGACAGTACAATTTGA", "aa": "MKNTKTSQEQVIELKLSDRQKKVLKSIVDEYTISATPVSSKLLVQKEFKDQSSATIRNEMMFLEKNGLIEKQHISGGRVPSLKGYDFYNKNLINKNNNVSDNFKMRLHKILSKRYSNIDEILNAAVSIINETTQLPAVVTKTSSDELLKRIDLVKINDNSALVLIVTSSENILTHSIKLDKNTKFNDLQTCFSVLDERLVDTKLSLISSKLDLIVDIVRNKLEEVEYYFSKIVHRVFDFYAYKPNIDSKTYGVKYLTKHSEFEDQQKLNDLLNLLEDSTI", "aa_hexdigest": "d3959ce7a6fe1ad2a50335f7d751f86f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "hrcA" ], "truncated": "3-prime", "pseudogene": { "start": 485044, "stop": 486117, "observations": { "mutation": [ 485884 ], "stop": [ 485884 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ68", "query_cov": 1.0, "subject_cov": 0.7843137254901961, "identity": 1.0, "score": 527.0, "evalue": 1.35e-187, "gene_start": 1, "gene_end": 280, "reference_start": 1, "reference_end": 280, "reference_sequence": "MKNTKTSQEQVIELKLSDRQKKVLKSIVDEYTISATPVSSKLLVQKEFKDQSSATIRNEMMFLEKNGLIEKQHISGGRVPSLKGYDFYNKNLINKNNNVSDNFKMRLHKILSKRYSNIDEILNAAVSIINETTQLPAVVTKTSSDELLKRIDLVKINDNSALVLIVTSSENILTHSIKLDKNTKFNDLQTCFSVLDERLVDTKLSLISSKLDLIVDIVRNKLEEVEYYFSKIVHRVFDFYAYKPNIDSKTYGVKYLTKHSEFEDQQKLNDLLNLLEDSTIWQQIALNKKTDGLAKIMLGNEIGHEHLAIATTQINLANTNHQITVVGPTRMDYAKIKALLDFLKIEIEKILGSTNEH" }, "paralog": false, "identity": 0.9971988795518207, "score": 686.0, "evalue": 1.67e-252, "description": "Internal stop codon at 485884. Nonsense mutation around 485884.", "psc": { "uniref90_id": "UniRef90_Q9PQ68", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_Q9PQ68", "cog_id": "COG1420", "cog_category": "K", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355", "GO:0045892" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ68", "UniRef:UniRef50_Q9PQ68", "COG:COG1420", "COG:K", "KEGG:K03705", "GO:0003677", "GO:0006355", "GO:0045892" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ68" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "GPKLONFDEH_860", "locus": "GCA000006625_00838" }, { "type": "cds", "sequence": "contig_1", "start": 485932, "stop": 486117, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGGAAATGAAATTGGCCACGAACATTTAGCCATTGCAACAACTCAAATTAATTTAGCAAACACTAATCATCAAATTACCGTTGTAGGTCCAACAAGAATGGATTATGCAAAAATCAAAGCTTTATTAGATTTTTTAAAAATTGAAATTGAAAAAATTTTAGGATCAACAAATGAACATTAA", "aa": "MLGNEIGHEHLAIATTQINLANTNHQITVVGPTRMDYAKIKALLDFLKIEIEKILGSTNEH", "aa_hexdigest": "29e3d29cbd33c1a35078f474d9531765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6780.739199999998, "isoelectric_point": 6.008870506286621 }, "id": "GPKLONFDEH_861", "locus": "GCA000006625_00839" }, { "type": "cds", "sequence": "contig_1", "start": 486107, "stop": 486268, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACATTAAAAAAATTAAACGTGAATTTAATACTCATTACGAATTGATTAAAAAAGAAATTAAAAACTTAAATGAATATAAAAAAGAATTAGAATTCTTAATTGCAACAATTTATTTTAATTCTAAAATGGTTTTTAAAGAAATATCATTAGATAGTTGA", "aa": "MNIKKIKREFNTHYELIKKEIKNLNEYKKELEFLIATIYFNSKMVFKEISLDS", "aa_hexdigest": "de1a08700e112986ac3bba80e86550e0", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6514.608299999998, "isoelectric_point": 9.26070613861084 }, "id": "GPKLONFDEH_862", "locus": "GCA000006625_00840" }, { "type": "cds", "sequence": "contig_1", "start": 486404, "stop": 486610, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3H6", "UniRef:UniRef90_A0AAC9T3H6" ], "nt": "TTGAATAAGCAATTGGAACAAATATATTTGAAATACATAATTTTTTATTGGTATAACATTAATATTCCAATAAATTATGAAATTGATGATTTTAATATTATTTGTTTACGCAATAAAGCATTTGTAGATTGTATGCAAATTTTACAAGAATATTATCATATTAATGTTGATATTTTAATTAATTTAGTTATCAAACAAATTAGATAA", "aa": "MNKQLEQIYLKYIIFYWYNINIPINYEIDDFNIICLRNKAFVDCMQILQEYYHINVDILINLVIKQIR", "aa_hexdigest": "a48b86ed9aa72feef5c4ead13195e46a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 486107, "stop": 486610, "observations": { "mutation": [ 486266, 486350 ], "stop": [ 486266, 486350 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T3H6", "query_cov": 1.0, "subject_cov": 0.40718562874251496, "identity": 0.985, "score": 136.0, "evalue": 1.15e-39, "gene_start": 1, "gene_end": 68, "reference_start": 100, "reference_end": 167, "reference_sequence": "MNIKKIKREFNTHYELIKKEIKNLNEYKKELEFLIATIYFNSKMVFKEISLDSWKKFSDYVYRFFLIINKNNQSQNFDLDCWYLEIKELIKVLNISNFLLNKQLEQIYLKYIIFYWYNINIPINYEIDDFNIICLRNKAFVDCMQILQEYYHINVDILINLVIKQIR" }, "paralog": false, "identity": 0.9880239520958084, "score": 331.0, "evalue": 5.5e-119, "description": "Internal stop codon at 486266, 486350. Nonsense mutation around 486266, 486350.", "psc": { "uniref90_id": "UniRef90_A0AAC9T3H6", "uniref50_id": "UniRef50_A0AAC9T3H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T3H6", "UniRef:UniRef50_A0AAC9T3H6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T3H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T3H6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8461.890799999997, "isoelectric_point": 5.586613655090332 }, "id": "GPKLONFDEH_863", "locus": "GCA000006625_00841" }, { "type": "cds", "sequence": "contig_1", "start": 486622, "stop": 486723, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAAAAAACAAACAAAGAATGAAAATTTTCAAGATCTAGATCAATTAAAAATTGGTGATGAACTAATGGTGCATGCTTATAAACTTAATGGTTGA", "aa": "MQEKKQTKNENFQDLDQLKIGDELMVHAYKLNG", "aa_hexdigest": "f6c1f542bd7ac05f70837bfd1a1aae8a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3907.3872999999994, "isoelectric_point": 5.564105415344237 }, "id": "GPKLONFDEH_864", "locus": "GCA000006625_00842" }, { "type": "cds", "sequence": "contig_1", "start": 486856, "stop": 487026, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTATTGGTTCTTTTTTAAAAATGAGTGGTTTAATTTAATTGCAACGGTTGAAAATGATGGTATCAAGTTTTATATTAATGTTGCATCTCCTTTTATTTATGAAGAGCAAGCAGTTAAGTATTATGATTTTGATTTAGATTTTAAAATTTCATCAGATGATAAATGA", "aa": "MSYWFFFKNEWFNLIATVENDGIKFYINVASPFIYEEQAVKYYDFDLDFKISSDDK", "aa_hexdigest": "18d0871c2a9c9dd31661d92781ebbe2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6853.578500000001, "isoelectric_point": 4.2393590927124025 }, "id": "GPKLONFDEH_865", "locus": "GCA000006625_00843" }, { "type": "cds", "sequence": "contig_1", "start": 487087, "stop": 487245, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTCAAAAAATTTTAAGCGTTGAAACTAAAATTGAAAATTATATTAAAGAAGGTTATTTTCGTAAACTTGTCACTCGTCAATTATTGATTAAACTACACGTTTTAGATGATATTGATGATTTTAAAACTAATTTTAATAAACACACTAATGAATGA", "aa": "MIQKILSVETKIENYIKEGYFRKLVTRQLLIKLHVLDDIDDFKTNFNKHTNE", "aa_hexdigest": "6510b2cf80313e00900e051fa8267aab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6294.238, "isoelectric_point": 8.051214790344236 }, "id": "GPKLONFDEH_866", "locus": "GCA000006625_00844" }, { "type": "cds", "sequence": "contig_1", "start": 487280, "stop": 488089, "strand": "+", "frame": 2, "gene": null, "product": "DUF3196 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C63", "UniRef:UniRef100_A0A2C9DYK7", "UniRef:UniRef50_A0A2C9DYK7", "UniRef:UniRef90_A0A2C9DYK7" ], "nt": "ATGGTTTGCATCATTTTAAAAATAAAAAAGGGAATGTTAAAGATAATGAATAATAATAAAAGCTACATTAAAAAATATTTTGATGATATTTTAATACAGGCTAAACAGGTTTTAAAAAAACATGATTATGCGTATGCATATGATTTAATTTCTAATGAATTTAATAATCCATTAATTGATTTAAAAACTTTACAAGAATTTGAAGCATTTGCTATAGAAATTAAAAAAAGTGCCGAACTTAACTTTATTGATGAAAATGAGGCAAAATTAGCTAAAACAGATTTTTATTGTAAAATTCATGATCCAAAAACAACTTATGTTAGTTTAGCATATTTAGAAACTTTTTTAATGCGTTTCATTAATGAAATGGATGAATTAGATATTACATTTTTAAATAGCTTGCTAACAAATAAATTAATTAATGGATCAACAAAATTAGATATTTTAGATTTATTAGCAACTAATAATATTGATCAAAATTTTAATTTTTACAATAAATATTTAAAACAAACAAAAAATGTTAACCCTGTTAATTTTAATAACCATTACCAAGAAATTGACCAAATTCAAAATTATCTAAATAAGTATTTAGCTAAAAATCCTTCTTTATTACATTTAGCCAATAAATTATTAATGATGTACATTACATATTATTTTCCATTTAAATTTGCACATAATCATAATTTAATTGCTAAAACAATTATTGATTATGTAAAAAATGCTATGGAAAATCATGTTTGTCAGTATAGTGAAGAACAAAAAGCTATTGTGGCTTGTATTAATAAAATTTTAGAAGAAGAGCAAGTATAA", "aa": "MVCIILKIKKGMLKIMNNNKSYIKKYFDDILIQAKQVLKKHDYAYAYDLISNEFNNPLIDLKTLQEFEAFAIEIKKSAELNFIDENEAKLAKTDFYCKIHDPKTTYVSLAYLETFLMRFINEMDELDITFLNSLLTNKLINGSTKLDILDLLATNNIDQNFNFYNKYLKQTKNVNPVNFNNHYQEIDQIQNYLNKYLAKNPSLLHLANKLLMMYITYYFPFKFAHNHNLIAKTIIDYVKNAMENHVCQYSEEQKAIVACINKILEEEQV", "aa_hexdigest": "57bb5c20ea82b7dc40b1890d00d04094", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C63", "uniref100_id": "UniRef100_A0A2C9DYK7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C63", "UniRef:UniRef100_A0A2C9DYK7" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYK7", "uniref90_id": "UniRef90_A0A2C9DYK7" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DYK7", "product": "DUF3196 domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYK7" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYK7" ], "product": "DUF3196 domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_867", "locus": "GCA000006625_00845" }, { "type": "cds", "sequence": "contig_1", "start": 488695, "stop": 488802, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACTAGCAATGATTTACTAATTTATTCTAATTTAAAACAAACACCTAAATACCAAATAGATAATAAAAAACTAAAAGAAGATTTTGTTAAAAATAATAATTGA", "aa": "MATSNDLLIYSNLKQTPKYQIDNKKLKEDFVKNNN", "aa_hexdigest": "7b358d5cce3fb963ad7a649d9c32e178", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4129.647600000002, "isoelectric_point": 9.158330345153807 }, "id": "GPKLONFDEH_868", "locus": "GCA000006625_00846" }, { "type": "cds", "sequence": "contig_1", "start": 489238, "stop": 489372, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAATCTTATAAAATTCGCACAAATTATGCAAGCGTACTAAATTCACTAAATTTATCATTAGATGCTAAAAATCAATTTAAAAAAAAAATTTTGAGCACACTTTTAGAATATACAATAAAACATTTTTAG", "aa": "MPKSYKIRTNYASVLNSLNLSLDAKNQFKKKILSTLLEYTIKHF", "aa_hexdigest": "da46d70384b4410b9ea88bb8124ab366", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5161.026799999998, "isoelectric_point": 9.998482322692869 }, "id": "GPKLONFDEH_869", "locus": "GCA000006625_00847" }, { "type": "cds", "sequence": "contig_1", "start": 490330, "stop": 490704, "strand": "-", "frame": 1, "gene": "ftnA", "product": "putative protein UU427", "db_xrefs": [ "COG:COG1528", "COG:P", "GO:0008199", "SO:0001217", "UniRef:UniRef50_Q56564", "UniRef:UniRef90_Q9PQ61" ], "nt": "ATGGTTAAATCTCAAAAAGTTATAGATGTTTTAAATACACATTATAATTTAAATTTAGAACTAGGTAGTATTTATGCACAATATGCTCATATTGCTGATGACCAATTCAGTATGCCTTTTTTAGCAAAATTTATTGCTGATTTAAGCAATGATAAATTAGGTGTTCACAAGAATTTAATTTCTGAATATGCTCGTAAAGTTGAAATTCCATTACACACTAAATTTAGTGTAGATGTTAATTTTAAGCCAGCGAATCCTAAGGAATTAATAAAACACATTTTAGATACAGAATTAAAAGTTCGTAAGCATGTAGCCAATATGGCAAAAGTATGTTTGGAAGAAAATGATTTTGAAACATTTAGTTTTGTAAAATGA", "aa": "MVKSQKVIDVLNTHYNLNLELGSIYAQYAHIADDQFSMPFLAKFIADLSNDKLGVHKNLISEYARKVEIPLHTKFSVDVNFKPANPKELIKHILDTELKVRKHVANMAKVCLEENDFETFSFVK", "aa_hexdigest": "54b29358093e8bc9698bc185ad99919b", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ftnA" ], "truncated": "5-prime", "pseudogene": { "start": 490192, "stop": 490704, "observations": { "mutation": [ 490332 ], "stop": [ 490332 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ61", "query_cov": 1.0, "subject_cov": 0.7294117647058823, "identity": 1.0, "score": 249.0, "evalue": 4.54e-83, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MVKSQKVIDVLNTHYNLNLELGSIYAQYAHIADDQFSMPFLAKFIADLSNDKLGVHKNLISEYARKVEIPLHTKFSVDVNFKPANPKELIKHILDTELKVRKHVANMAKVCLEENDFETFSFVKWFVDDGIKDFDDVRTIHDFFENATNNLQVEYAIRKYLKQMKVEEEK" }, "paralog": false, "identity": 0.9941176470588236, "score": 338.0, "evalue": 6.38e-121, "description": "Internal stop codon at 490332. Nonsense mutation around 490332.", "psc": { "uniref90_id": "UniRef90_Q9PQ61", "gene": "FtnA", "product": "Uncharacterized protein UU427", "uniref50_id": "UniRef50_Q56564", "cog_id": "COG1528", "cog_category": "P", "go_ids": [ "GO:0008199" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ61", "UniRef:UniRef50_Q56564", "COG:COG1528", "COG:P", "GO:0008199" ] }, "pscc": { "uniref50_id": "UniRef50_Q56564", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56564" ], "product": "Uncharacterized protein UU427 homolog" } }, "id": "GPKLONFDEH_870", "locus": "GCA000006625_00848" }, { "type": "cds", "sequence": "contig_1", "start": 490811, "stop": 491176, "strand": "-", "frame": 3, "gene": "ureD", "product": "Urease accessory protein UreD", "db_xrefs": [ "COG:COG0829", "COG:O", "GO:0005737", "GO:0016151", "KEGG:K03190", "SO:0001217", "UniRef:UniRef50_Q56562", "UniRef:UniRef90_Q56562" ], "nt": "ATGTATTTAAATACTAAAATATATTATGATGATAAATTGGTTTTATTTGATAATTTAAAATTTCAACCACGTAAAAATGATGAATCAGCATTTGGAATCATGGATGGCTATCATTATTGTGGAACCATGATTGTAATTAATCAACAAGTTATTGAAGATGATGTTATTAAAATTCGTGATTTAGTTAAGGAAAAATATCCTGACATGGATATGATATTTGGTGTATCACGTATGGATATTCCTGGTTTGGGATTACGAGTTTTAGCTAATACTTATTATCATGTTGAAAAAATTAATGCTGTTGCTCATGATTATTTTAGAAGAAAATTATTCAATAAAAAACCTTTAATTTTACGAAAACCATAA", "aa": "MYLNTKIYYDDKLVLFDNLKFQPRKNDESAFGIMDGYHYCGTMIVINQQVIEDDVIKIRDLVKEKYPDMDMIFGVSRMDIPGLGLRVLANTYYHVEKINAVAHDYFRRKLFNKKPLILRKP", "aa_hexdigest": "04e6261a7ffed0f5bcd48cbd34a35b2d", "start_type": "ATG", "rbs_motif": null, "genes": [ "ureD" ], "truncated": "3-prime", "pseudogene": { "start": 490811, "stop": 491476, "observations": { "mutation": [ 491212 ], "stop": [ 491212 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q56562", "query_cov": 1.0, "subject_cov": 0.42160278745644597, "identity": 1.0, "score": 251.0, "evalue": 2.32e-82, "gene_start": 1, "gene_end": 121, "reference_start": 167, "reference_end": 287, "reference_sequence": "MILNKEKIKNYAAYLYIKVAYDQAHSKMAHTVYFTNFYRSSKPLFLDEEDPINPCFQTISMGGGYVSGEIYRSDFEINDDARCIITTQSSAKAYKTVDGKTSEQHTNITLGKNSILEYISDNVIVYEDGKFAQFNNFKMDSSATLIYTECFGPGWSPHGSAYQYEKMYLNTKIYYDDKLVLFDNLKFQPRKNDESAFGIMDGYHYCGTMIVINQQVIEDDVIKIRDLVKEKYPDMDMIFGVSRMDIPGLGLRVLANTYYHVEKINAVAHDYFRRKLFNKKPLILRKP" }, "paralog": false, "identity": 0.995475113122172, "score": 449.0, "evalue": 5.62e-163, "description": "Internal stop codon at 491212. Nonsense mutation around 491212.", "psc": { "uniref90_id": "UniRef90_Q56562", "gene": "ureD", "product": "Urease accessory protein UreD", "uniref50_id": "UniRef50_Q56562", "cog_id": "COG0829", "cog_category": "O", "kegg_orthology_id": "K03190", "go_ids": [ "GO:0005737", "GO:0016151" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q56562", "UniRef:UniRef50_Q56562", "COG:COG0829", "COG:O", "KEGG:K03190", "GO:0005737", "GO:0016151" ] }, "pscc": { "uniref50_id": "UniRef50_Q56562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56562" ], "product": "Urease accessory protein UreD" } }, "id": "GPKLONFDEH_871", "locus": "GCA000006625_00849" }, { "type": "cds", "sequence": "contig_1", "start": 491210, "stop": 491674, "strand": "-", "frame": 3, "gene": "ureD", "product": "Urease accessory protein UreD", "db_xrefs": [ "COG:COG0829", "COG:O", "GO:0005737", "GO:0016151", "KEGG:K03190", "SO:0001217", "UniRef:UniRef50_Q56562", "UniRef:UniRef90_Q56562" ], "nt": "ATGATTTTAAATAAAGAAAAAATTAAAAATTATGCTGCTTATTTATACATTAAAGTAGCATATGATCAAGCTCATTCAAAAATGGCACATACTGTCTATTTTACGAATTTTTATCGTTCATCTAAACCTTTATTTTTAGATGAAGAAGATCCAATCAACCCCTGTTTTCAAACAATTAGTATGGGTGGTGGATATGTTTCAGGTGAAATTTATCGTTCTGATTTTGAAATTAATGATGATGCTCGTTGTATCATTACAACACAATCCTCAGCTAAAGCTTATAAAACGGTTGATGGAAAAACTTCTGAGCAACATACAAATATCACGTTAGGAAAAAATAGTATTTTAGAATATATAAGTGATAACGTAATTGTTTATGAAGATGGAAAGTTCGCACAATTTAATAATTTTAAAATGGATTCAAGTGCTACTTTAATTTACACAGAATGTTTTGGCCCTGGTTGA", "aa": "MILNKEKIKNYAAYLYIKVAYDQAHSKMAHTVYFTNFYRSSKPLFLDEEDPINPCFQTISMGGGYVSGEIYRSDFEINDDARCIITTQSSAKAYKTVDGKTSEQHTNITLGKNSILEYISDNVIVYEDGKFAQFNNFKMDSSATLIYTECFGPG", "aa_hexdigest": "207b417fe4ca05f1d3e94d704259cfcf", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [ "ureD" ], "truncated": "5-prime", "pseudogene": { "start": 490907, "stop": 491674, "observations": { "mutation": [ 491212 ], "stop": [ 491212 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q56562", "query_cov": 1.0, "subject_cov": 0.5365853658536586, "identity": 1.0, "score": 315.0, "evalue": 5.26e-107, "gene_start": 1, "gene_end": 154, "reference_start": 1, "reference_end": 154, "reference_sequence": "MILNKEKIKNYAAYLYIKVAYDQAHSKMAHTVYFTNFYRSSKPLFLDEEDPINPCFQTISMGGGYVSGEIYRSDFEINDDARCIITTQSSAKAYKTVDGKTSEQHTNITLGKNSILEYISDNVIVYEDGKFAQFNNFKMDSSATLIYTECFGPGWSPHGSAYQYEKMYLNTKIYYDDKLVLFDNLKFQPRKNDESAFGIMDGYHYCGTMIVINQQVIEDDVIKIRDLVKEKYPDMDMIFGVSRMDIPGLGLRVLANTYYHVEKINAVAHDYFRRKLFNKKPLILRKP" }, "paralog": false, "identity": 0.996078431372549, "score": 519.0, "evalue": 8e-190, "description": "Internal stop codon at 491212. Nonsense mutation around 491212.", "psc": { "uniref90_id": "UniRef90_Q56562", "gene": "ureD", "product": "Urease accessory protein UreD", "uniref50_id": "UniRef50_Q56562", "cog_id": "COG0829", "cog_category": "O", "kegg_orthology_id": "K03190", "go_ids": [ "GO:0005737", "GO:0016151" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q56562", "UniRef:UniRef50_Q56562", "COG:COG0829", "COG:O", "KEGG:K03190", "GO:0005737", "GO:0016151" ] }, "pscc": { "uniref50_id": "UniRef50_Q56562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56562" ], "product": "Urease accessory protein UreD" } }, "id": "GPKLONFDEH_872", "locus": "GCA000006625_00850" }, { "type": "cds", "sequence": "contig_1", "start": 491723, "stop": 492304, "strand": "-", "frame": 3, "gene": "ureG", "product": "urease accessory protein UreG", "db_xrefs": [ "COG:COG0378", "COG:O", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0016151", "GO:0016530", "GO:0019627", "GO:0043419", "GO:0046872", "KEGG:K03189", "SO:0001217", "UniRef:UniRef50_B1XYX6", "UniRef:UniRef90_Q56561" ], "nt": "ATGAAAAGACCATTAATTATTGGTGTAGGTGGACCTGTAGGAGCAGGTAAAACAATGCTAATCGAAAGATTAACAAGATACCTTTCAACAAAAGGTTATAGTATGGCAGCAATTACTAATGATATTTACACTAAAGAAGATGCTAGAATTTTATTAAATACTTCTGTTTTACCAGCTGATCGTATTGCTGGTGTTGAAACAGGAGGATGTCCACATACAGCAATTCGTGAAGATGCTTCAATGAACTTTGCTGCAATTGAAGAAATGTGTGATAAACACCCTGATTTACAATTATTATTTTTAGAATCTGGTGGGGATAATTTATCTGCAACATTTAGTCCAGATTTAGTTGATTTTTCAATTTATATCATTGATGTTGCACAAGGAGAAAAAATTCCTCGTAAGGGTGGTCAAGGAATGATTAAATCAGACTTATTTATTATTAATAAAGTTGATTTAGCTCCTTATGTTGGTGCTAATGTAGAAGTAATGAAAGCCGATACATTAAAATCACGTGGTAATAAAGATTTCTTTGTAACGAATTTAAAAACAGATGAAGGTTTAAAATCTGTTGCTGATTGA", "aa": "MKRPLIIGVGGPVGAGKTMLIERLTRYLSTKGYSMAAITNDIYTKEDARILLNTSVLPADRIAGVETGGCPHTAIREDASMNFAAIEEMCDKHPDLQLLFLESGGDNLSATFSPDLVDFSIYIIDVAQGEKIPRKGGQGMIKSDLFIINKVDLAPYVGANVEVMKADTLKSRGNKDFFVTNLKTDEGLKSVAD", "aa_hexdigest": "376ee03b3e0b6332264fba5f0245eb77", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q56561", "query_cov": 1.0, "subject_cov": 0.9368932038834952, "identity": 1.0, "score": 384.0, "evalue": 8.3e-135, "valid": true, "gene": "ureG", "product": "urease accessory protein UreG", "uniref50_id": "UniRef50_B1XYX6", "cog_id": "COG0378", "cog_category": "O", "kegg_orthology_id": "K03189", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0016151", "GO:0016530", "GO:0019627", "GO:0043419", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_B1XYX6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1XYX6" ], "product": "Urease accessory protein UreG" }, "genes": [ "ureG" ], "id": "GPKLONFDEH_873", "locus": "GCA000006625_00851" }, { "type": "cds", "sequence": "contig_1", "start": 492386, "stop": 492832, "strand": "-", "frame": 3, "gene": "ureF", "product": "Urease accessory protein UreF", "db_xrefs": [ "GO:0005737", "GO:0016151", "KEGG:K03188", "SO:0001217", "UniRef:UniRef50_Q56560", "UniRef:UniRef90_Q56560" ], "nt": "ATGATAAACTTTCAAGGTTTAGCAAGAGAGACTCGTGAAGGTCAACGTCGAATTGGACAACAAATGGTAAAGATATATAATGAACTTTTTAATTGTGAACTTTTAGTTGAATATGCTGAAAGAATAAAAAATAAAAAATCTTATGGAAATCCTGCTGTTGCGTTTGCTTTATTAGCTATGCATTTAAAAATAGATTTAAAAACTGCTTTATATACGCATCTTTACTCTACAGTTGCTGCATTAACGCAAAACTGTGTACGTGCAATTCCATTAGGACAAGTTAAGGGACAAAAAATAATTTATCAACTTAAACATGTTTATTTTGATGATATTGTTAATAAAGTCTTTACATTGGATTTTAAAACAGATTTTTGCAAAAATATACCAGGCCTTGAAATTGCACAAATGGAACACGAAGACACACCTGTTCGATTATTCATGTCATAA", "aa": "MINFQGLARETREGQRRIGQQMVKIYNELFNCELLVEYAERIKNKKSYGNPAVAFALLAMHLKIDLKTALYTHLYSTVAALTQNCVRAIPLGQVKGQKIIYQLKHVYFDDIVNKVFTLDFKTDFCKNIPGLEIAQMEHEDTPVRLFMS", "aa_hexdigest": "b0b677bfc303a8984861792e180cbc90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ureF" ], "truncated": "3-prime", "pseudogene": { "start": 492386, "stop": 493132, "observations": { "mutation": [ 492847 ], "stop": [ 492847 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q56560", "query_cov": 1.0, "subject_cov": 0.592, "identity": 1.0, "score": 299.0, "evalue": 2.89e-101, "gene_start": 1, "gene_end": 148, "reference_start": 103, "reference_end": 250, "reference_sequence": "MKEKKLNLKKHLNTVVTLNSDYLNLLDLMQITNANFPIGTFSHSFGVETYIRKDIVFDGESLIKALLLYMNEQLLHGDLLAIYQIFKLLPKQKINAIWEIDQMINFQGLARETREGQRRIGQQMVKIYNELFNCELLVEYAERIKNKKSYGNPAVAFALLAMHLKIDLKTALYTHLYSTVAALTQNCVRAIPLGQVKGQKIIYQLKHVYFDDIVNKVFTLDFKTDFCKNIPGLEIAQMEHEDTPVRLFMS" }, "paralog": false, "identity": 0.9959677419354839, "score": 489.0, "evalue": 8.69e-179, "description": "Internal stop codon at 492847. Nonsense mutation around 492847.", "psc": { "uniref90_id": "UniRef90_Q56560", "gene": "ureF", "product": "Urease accessory protein UreF", "uniref50_id": "UniRef50_Q56560", "kegg_orthology_id": "K03188", "go_ids": [ "GO:0005737", "GO:0016151" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q56560", "UniRef:UniRef50_Q56560", "KEGG:K03188", "GO:0005737", "GO:0016151" ] }, "pscc": { "uniref50_id": "UniRef50_Q56560", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56560" ], "product": "Urease accessory protein UreF" } }, "id": "GPKLONFDEH_874", "locus": "GCA000006625_00852" }, { "type": "cds", "sequence": "contig_1", "start": 492845, "stop": 493138, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAAAAAAATTAAACTTAAAGAAGCATTTAAACACTGTAGTGACGCTAAATAGTGACTATTTAAATTTATTAGACCTAATGCAGATCACTAACGCAAACTTTCCAATTGGAACATTTAGTCATTCTTTTGGAGTTGAAACTTATATTAGAAAAGATATTGTTTTTGATGGAGAATCATTAATTAAAGCGTTACTTCTGTATATGAATGAACAATTGTTACATGGTGATTTATTGGCAATTTATCAAATTTTTAAACTATTGCCAAAACAAAAAATAAATGCAATTTGA", "aa": "MKEKKLNLKKHLNTVVTLNSDYLNLLDLMQITNANFPIGTFSHSFGVETYIRKDIVFDGESLIKALLLYMNEQLLHGDLLAIYQIFKLLPKQKINAI", "aa_hexdigest": "33df8464cf85b9a707be397b1943738c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11179.077399999987, "isoelectric_point": 8.893365287780764 }, "id": "GPKLONFDEH_875", "locus": "GCA000006625_00853" }, { "type": "cds", "sequence": "contig_1", "start": 493080, "stop": 493529, "strand": "-", "frame": 2, "gene": "ureE", "product": "Urease accessory protein UreE", "db_xrefs": [ "COG:COG2371", "COG:O", "GO:0005737", "GO:0006457", "GO:0016151", "GO:0019627", "GO:0051082", "GO:0065003", "RefSeq:WP_006688775.1", "SO:0001217", "UniParc:UPI000000090C", "UniRef:UniRef100_Q56559", "UniRef:UniRef50_Q56559", "UniRef:UniRef90_Q56559" ], "nt": "TTGACTGTATTTAAAGAAATTTTAGATAATATTGCTAACATTAAAAATGTTGAAAGTTATCAAATTGAGAACATTCATTTAACAAGCGACGACGTTTTAAAACGTGTAATTATCATTTCATCAGATCAAAATGTTGAATATGGAATTCGTTTAGAAGAGGACAAAAAATTAATGGATGGTGACATCTTATACAAGGATGATTATAAACTAGTTGTTATTAGATTAGAGTTATCAGATGTATTAATTATTACAGCACACACAATTGGTGAAATGGCAAAAATTGCCCATAATTTAGGTAATCGTCATATGCCGGCTCAATTTACTGAAACACAAATGATCGTTCCCTACGACTATTTAGTAGAAGAATACCTTCAAGATAATAAAGCCCTATATGAAAGAAAAAAAATTAAACTTAAAGAAGCATTTAAACACTGTAGTGACGCTAAATAG", "aa": "MTVFKEILDNIANIKNVESYQIENIHLTSDDVLKRVIIISSDQNVEYGIRLEEDKKLMDGDILYKDDYKLVVIRLELSDVLIITAHTIGEMAKIAHNLGNRHMPAQFTETQMIVPYDYLVEEYLQDNKALYERKKIKLKEAFKHCSDAK", "aa_hexdigest": "173e1a5c166619171c4642ed5e321a09", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI000000090C", "ncbi_nrp_id": "WP_006688775.1", "uniref100_id": "UniRef100_Q56559", "db_xrefs": [ "SO:0001217", "UniParc:UPI000000090C", "RefSeq:WP_006688775.1", "UniRef:UniRef100_Q56559" ] }, "ips": { "uniref100_id": "UniRef100_Q56559", "uniref90_id": "UniRef90_Q56559" }, "psc": { "uniref90_id": "UniRef90_Q56559", "gene": "ureE", "product": "Urease accessory protein UreE", "uniref50_id": "UniRef50_Q56559", "cog_id": "COG2371", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0016151", "GO:0051082", "GO:0006457", "GO:0065003", "GO:0019627" ] }, "pscc": { "uniref50_id": "UniRef50_Q56559", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q56559" ], "product": "Urease accessory protein UreE" }, "genes": [ "ureE" ], "id": "GPKLONFDEH_876", "locus": "GCA000006625_00854" }, { "type": "cds", "sequence": "contig_1", "start": 493792, "stop": 494025, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTAATCGCTCGTTGTGTAGCAGGTGATCCAAATGCTTCAATTCCAACATGCGAACCTGTAATTATGCGTGACCAATTTGGAACTTATGGACGTTTGTTAACTAATACATCAGTAAGTTTTGTTTCAAAAATTGGGTTAGAAAATGGCATTAAAGAGGAATATAAATTAGAAAAAGAATTATTACCAGTTAAAAATTGCCGTTCAGTAAATAAAAAGAGTATGAAATGA", "aa": "MGVIARCVAGDPNASIPTCEPVIMRDQFGTYGRLLTNTSVSFVSKIGLENGIKEEYKLEKELLPVKNCRSVNKKSMK", "aa_hexdigest": "2da4a626ff70b4370d195d9b22e46ec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8504.880499999992, "isoelectric_point": 9.151754570007324 }, "id": "GPKLONFDEH_877", "locus": "GCA000006625_00855" }, { "type": "cds", "sequence": "contig_1", "start": 494068, "stop": 494244, "strand": "-", "frame": 1, "gene": null, "product": "amidohydrolase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00374938FA" ], "nt": "ATGGCTCACAAAATGAAAGCACAATTTGGATCATTAAAAGGTGATAGTGAATTCAGTGATAACAATCGTGTAAAACGTTATATTTCTAAATATACAATTAACCCAGCTATTGCACATGGTGTTGATTCTTATATTGGTTCACTAGAAGTTGGTAAATTAGCTGATATTGTTGCTTGA", "aa": "MAHKMKAQFGSLKGDSEFSDNNRVKRYISKYTINPAIAHGVDSYIGSLEVGKLADIVA", "aa_hexdigest": "3d2094ac8b60e40703e157618b050ae4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00374938FA", "query_cov": 0.9137931034482759, "subject_cov": 0.9814814814814815, "identity": 0.66, "score": 72.0, "evalue": 1.2e-15, "valid": false, "product": "amidohydrolase family protein", "uniref50_id": "UniRef50_UPI00374938FA" }, "pscc": { "uniref50_id": "UniRef50_UPI00374938FA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00374938FA" ], "product": "amidohydrolase family protein" }, "genes": [], "id": "GPKLONFDEH_878", "locus": "GCA000006625_00856" }, { "type": "cds", "sequence": "contig_1", "start": 494248, "stop": 494586, "strand": "-", "frame": 1, "gene": null, "product": "amidohydrolase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021476868" ], "nt": "ATGAAAGGGCGAACAATTCATGCTTATCATACAGAAGGTGCTGGTGGAGGGCATGCTCCAGATATTCTAGAAACTGTTAAATATGCCCATATTTTACCAGCTTCTACAAACCCAACAATTCCTTATACAGTAAATACAATTGCTGAACATTTAGATATGTTAATGGTATGTCACCACTTAAATCCTAAGGTTCCAGAAGATGTTGCTTTTGCTGATTCACGTATTCGTAGCCAAACAATTGCAGCTGAAGACTTATTGCACGATATGGGTGCAATTTCAATTATGTCATCAGATACATTAGCTATGGGACGTATTGGCGAAGTTGCAACTCGTACATGA", "aa": "MKGRTIHAYHTEGAGGGHAPDILETVKYAHILPASTNPTIPYTVNTIAEHLDMLMVCHHLNPKVPEDVAFADSRIRSQTIAAEDLLHDMGAISIMSSDTLAMGRIGEVATRT", "aa_hexdigest": "93f1bdfc3f78033ad4d79aeed2e028a3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021476868", "query_cov": 1.0, "subject_cov": 0.8175182481751825, "identity": 0.705, "score": 173.0, "evalue": 9.8e-54, "valid": false, "product": "amidohydrolase family protein", "uniref50_id": "UniRef50_UPI0021476868" }, "pscc": { "uniref50_id": "UniRef50_UPI0021476868", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021476868" ], "product": "amidohydrolase family protein" }, "genes": [], "id": "GPKLONFDEH_879", "locus": "GCA000006625_00857" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 494943, "stop": 495212, "strand": "+", "gene": "5_ureB_sRNA", "product": "5' ureB small RNA", "score": 62.9, "evalue": 1.8e-07, "db_xrefs": [ "RFAM:RF02514", "SO:0000655" ], "nt": "CTATTTCTGGTTCTAGTCAGTGAACGTGAGTATCTAAACCACCAGCTGTATAAATTTTACCCTCACCAGCTGAAATTTCAGTTGAGATACCCACAATCATATCAACATTATCTGTTAAATGTGGATTTCCAGATTTACCAATCGCAGCAATTTTTCCATTTTTAATACCAATATCTGCTTTATAAATACCTGTATAATCAACAATTAGTGCATTTGTAATAACTAAATCCATTACTTCAGCATTACCTAATTTATCATCTAACTTCATAG", "id": "GPKLONFDEH_39", "locus": "GCA000006625_00858" }, { "type": "cds", "sequence": "contig_1", "start": 494959, "stop": 495222, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00449.26" ], "nt": "ATGAATTCTACTATGAAGTTAGATGATAAATTAGGTAATGCTGAAGTAATGGATTTAGTTATTACAAATGCACTAATTGTTGATTATACAGGTATTTATAAAGCAGATATTGGTATTAAAAATGGAAAAATTGCTGCGATTGGTAAATCTGGAAATCCACATTTAACAGATAATGTTGATATGATTGTGGGTATCTCAACTGAAATTTCAGCTGGTGAGGGTAAAATTTATACAGCTGGTGGTTTAGATACTCACGTTCACTGA", "aa": "MNSTMKLDDKLGNAEVMDLVITNALIVDYTGIYKADIGIKNGKIAAIGKSGNPHLTDNVDMIVGISTEISAGEGKIYTAGGLDTHVH", "aa_hexdigest": "8d67e1be2709cae77a8eee147e5cc334", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00449.26", "name": "Urease_alpha", "length": 67, "aa_cov": 0.7471264367816092, "hmm_cov": 1.0, "evalue": 2.246103337787685e-25, "score": 84.91765594482422, "start": 5, "stop": 69 } ], "seq_stats": { "molecular_weight": 9130.326199999998, "isoelectric_point": 5.060000419616698 }, "id": "GPKLONFDEH_880", "locus": "GCA000006625_00859" }, { "type": "cds", "sequence": "contig_1", "start": 495475, "stop": 495810, "strand": "-", "frame": 1, "gene": "ureB", "product": "urease subunit beta", "db_xrefs": [ "COG:COG0832", "COG:E", "EC:3.5.1.5", "GO:0009039", "GO:0035550", "GO:0043419", "SO:0001217", "UniRef:UniRef50_P0C7K8", "UniRef:UniRef90_P0C7K8" ], "nt": "ATGTCAGGATCATCAAGTCAATTTAGTCCAGGTAAATTAGTACCAGGGGCAATTAATTTCGCTAGTGGTGAAATTGTGATGAATGAAGGTAGAGAGGCAAAAGTAATTAGTATTAAAAATACTGGGGACCGTCCTATACAAGTTGGATCACATTTTCACTTGTTTGAAGTGAATAGTGCATTAGTATTTTTTGATGAAAAAGGAAATGAAGATAAAGAACGCAAAGTTGCTTATGGACGACGTTTCGATATTCCATCAGGTACTGCTATTCGTTTTGAACCAGGAGATAAAAAAGAAGTTTCAATTATTGATTTAGCCGGAACACGCGAAGTTTGA", "aa": "MSGSSSQFSPGKLVPGAINFASGEIVMNEGREAKVISIKNTGDRPIQVGSHFHLFEVNSALVFFDEKGNEDKERKVAYGRRFDIPSGTAIRFEPGDKKEVSIIDLAGTREV", "aa_hexdigest": "af1e5422020534dcb24248128f748aeb", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_P0C7K8", "query_cov": 1.0, "subject_cov": 0.8951612903225806, "identity": 1.0, "score": 220.0, "evalue": 1.1e-72, "valid": true, "gene": "ureB", "product": "urease subunit beta", "ec_ids": [ "3.5.1.5" ], "uniref50_id": "UniRef50_P0C7K8", "cog_id": "COG0832", "cog_category": "E", "go_ids": [ "GO:0009039", "GO:0035550", "GO:0043419" ] }, "pscc": { "uniref50_id": "UniRef50_P0C7K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0C7K8" ], "product": "Urease subunit beta" }, "genes": [ "ureB" ], "id": "GPKLONFDEH_881", "locus": "GCA000006625_00860" }, { "type": "cds", "sequence": "contig_1", "start": 495857, "stop": 496162, "strand": "-", "frame": 3, "gene": "ureA", "product": "urease subunit gamma", "db_xrefs": [ "COG:COG0831", "COG:E", "EC:3.5.1.5", "GO:0005737", "GO:0009039", "GO:0016151", "GO:0043419", "RefSeq:WP_010891770.1", "SO:0001217", "UniParc:UPI0000137DA3", "UniRef:UniRef100_P0C7K9", "UniRef:UniRef50_Q8UCS6", "UniRef:UniRef90_P0C7K9" ], "nt": "ATGAATCTATCATTAAGAGAAGTCCAAAAATTATTGATAACAGTTGCTGCTGACGTTGCAAGAAGACGTTTAGCTAGAGGTTTAAAATTAAACTATTCAGAAGCTGTTGCTTTAATTACTGATCATGTAATGGAAGGGGCAAGAGATGGTAAGCTAGTTGCTGACCTAATGCAATCTGCTCGTGAAGTACTACGTGTTGATCAAGTTATGGAAGGTGTAGATACAATGGTTAGTATAATTCAAGTTGAAGTTACTTTCCCTGATGGTACTAAACTAGTTTCTGTACACGATCCAATTTACAAATAA", "aa": "MNLSLREVQKLLITVAADVARRRLARGLKLNYSEAVALITDHVMEGARDGKLVADLMQSAREVLRVDQVMEGVDTMVSIIQVEVTFPDGTKLVSVHDPIYK", "aa_hexdigest": "d4c1bee26cc79ea600110a2e09b83079", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000137DA3", "ncbi_nrp_id": "WP_010891770.1", "uniref100_id": "UniRef100_P0C7K9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137DA3", "RefSeq:WP_010891770.1", "UniRef:UniRef100_P0C7K9" ] }, "ips": { "uniref100_id": "UniRef100_P0C7K9", "uniref90_id": "UniRef90_P0C7K9" }, "psc": { "uniref90_id": "UniRef90_P0C7K9", "gene": "ureA", "product": "urease subunit gamma", "ec_ids": [ "3.5.1.5" ], "uniref50_id": "UniRef50_Q8UCS6", "cog_id": "COG0831", "cog_category": "E", "go_ids": [ "GO:0005737", "GO:0009039", "GO:0016151", "GO:0043419" ] }, "pscc": { "uniref50_id": "UniRef50_Q8UCS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8UCS6" ], "product": "Urease subunit gamma" }, "genes": [ "ureA" ], "id": "GPKLONFDEH_882", "locus": "GCA000006625_00861" }, { "type": "cds", "sequence": "contig_1", "start": 496464, "stop": 496820, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAGTTTTTTTAAAAAAAAGAATGATTTACAAAAAGAAGAACACAAACACGTTAGTGTTGTAGATATGAATGAAATTTTAAGCTATGAAGATAGTGAAGAATTTAAAAACGATGAAATTGATTCTAATGAGCCAATTTTTAAACTTAATAATGTTTCATTAGGTTACGGAAAAAAAATGATTATTAAAAACCTAACGGCAACAATTCATCGCAACGATTTTGTTGTTGTATTAGGGCCTAATGGTTCTGGTAAATCCACATTAATCAAAGGATTGTGTCGTATCAATAATCCTTCAAGTGGTTATATAATGTATAGAGATAAATTGATATCAAGACCATTATTACCATTTCTTTGA", "aa": "MSFFKKKNDLQKEEHKHVSVVDMNEILSYEDSEEFKNDEIDSNEPIFKLNNVSLGYGKKMIIKNLTATIHRNDFVVVLGPNGSGKSTLIKGLCRINNPSSGYIMYRDKLISRPLLPFL", "aa_hexdigest": "d4db79aba6596f8fd6505ad150503235", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 49, "aa_cov": 0.4152542372881356, "hmm_cov": 1.0, "evalue": 1.312982223125824e-11, "score": 40.842254638671875, "start": 63, "stop": 111 } ], "seq_stats": { "molecular_weight": 13506.419199999977, "isoelectric_point": 8.646064567565919 }, "id": "GPKLONFDEH_883", "locus": "GCA000006625_00862" }, { "type": "cds", "sequence": "contig_1", "start": 497016, "stop": 497627, "strand": "+", "frame": 3, "gene": "fepC", "product": "Ferrichrome transport ATP-binding protein", "db_xrefs": [ "COG:COG1120", "COG:PH", "SO:0001217", "UniRef:UniRef50_A0A084EXZ1", "UniRef:UniRef90_Q9PQ55" ], "nt": "ATGGGTCGTTTTCCAAGTTCAAATGCTTTGGGAATTAATACAAATACAGAACGTGAAAAACAAATTATTGATGAAGCTTTGAAAAATGTTGGTATTTATGAATTCCGCCACAAAAATCTTGAAGATTTATCAGGTGGTCAAAAACAAAAAGCATTAATTGCTTTAGCACTTGCTCAAGATACTGAAACAATTGTTTTAGATGAACCTACTAACCACTTAGATATAAGAAGTCAATTAGAAATTATTGAACTTTTACATAAATTACACCACGAAATGAAAAAAACAATTGTTTTAGTTATTCATGATATTAATAATGGTTTAAAATATGCACATAAAGTCATGATTATGAAAAATGGTGAAATGGTTCGTTATGGAAAATTAAAAGAAACAATCGATCATGAAATTTTATTAGATGTTTTTGGTGTTGAATCAATTATTGTGAAAAATGAATCGACATATCCACAAGTTTCTGTAACAGATTTTAGTTTACCAAAAGACTATAAAATTAATGAAATCAAAGAAAACGAAAAAACTAATTCAATTTATAATGATGGTGATGAAGTAGAAATTGAAAATGAATTAGAAAAAGAAGTTGATAAAACAAAAAAATAA", "aa": "MGRFPSSNALGINTNTEREKQIIDEALKNVGIYEFRHKNLEDLSGGQKQKALIALALAQDTETIVLDEPTNHLDIRSQLEIIELLHKLHHEMKKTIVLVIHDINNGLKYAHKVMIMKNGEMVRYGKLKETIDHEILLDVFGVESIIVKNESTYPQVSVTDFSLPKDYKINEIKENEKTNSIYNDGDEVEIENELEKEVDKTKK", "aa_hexdigest": "b29b32418c5f0c8efa145c4c70ca7496", "start_type": "ATG", "rbs_motif": null, "genes": [ "fepC" ], "truncated": "5-prime", "pseudogene": { "start": 496716, "stop": 497627, "observations": { "mutation": [ 496818, 496833, 496893 ], "stop": [ 496818, 496833, 496893 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ55", "query_cov": 1.0, "subject_cov": 0.5218508997429306, "identity": 1.0, "score": 391.0, "evalue": 9.53e-135, "gene_start": 1, "gene_end": 203, "reference_start": 185, "reference_end": 387, "reference_sequence": "MSFFKKKNDLQKEEHKHVSVVDMNEILSYEDSEEFKNDEIDSNEPIFKLNNVSLGYGKKMIIKNLTATIHRNDFVVVLGPNGSGKSTLIKGLCRINNPSSGYIMYRDKLISRPLLPFLWLEKAWSSIVNIFTKDRKEVIETIDWHIQNYKKIHAYKSKELALNLAYVPQLAVFPEATSIYDFVKMGRFPSSNALGINTNTEREKQIIDEALKNVGIYEFRHKNLEDLSGGQKQKALIALALAQDTETIVLDEPTNHLDIRSQLEIIELLHKLHHEMKKTIVLVIHDINNGLKYAHKVMIMKNGEMVRYGKLKETIDHEILLDVFGVESIIVKNESTYPQVSVTDFSLPKDYKINEIKENEKTNSIYNDGDEVEIENELEKEVDKTKKIN" }, "paralog": false, "identity": 0.9900990099009901, "score": 586.0, "evalue": 7.35e-214, "description": "Internal stop codon at 496818, 496833, 496893. Nonsense mutation around 496818, 496833, 496893.", "psc": { "uniref90_id": "UniRef90_Q9PQ55", "gene": "FepC", "product": "Ferrichrome transport ATP-binding protein", "uniref50_id": "UniRef50_A0A084EXZ1", "cog_id": "COG1120", "cog_category": "PH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ55", "UniRef:UniRef50_A0A084EXZ1", "COG:COG1120", "COG:PH" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084EXZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EXZ1" ], "product": "ABC transporter ATP-binding protein" } }, "id": "GPKLONFDEH_884", "locus": "GCA000006625_00863" }, { "type": "cds", "sequence": "contig_1", "start": 497710, "stop": 498453, "strand": "+", "frame": 1, "gene": "yqxC", "product": "Hemolysin", "db_xrefs": [ "COG:COG1189", "COG:J", "EC:2.1.1.226", "EC:2.1.1.227", "KEGG:K06442", "RefSeq:WP_006689051.1", "SO:0001217", "UniParc:UPI00000C1C68", "UniRef:UniRef100_Q9PQ54", "UniRef:UniRef50_Q9PQ54", "UniRef:UniRef90_Q9PQ54" ], "nt": "ATGAATCAAAGGTTAGATCATTATCTGAAAAACACTAATCAATGTGAAACACGATCCAAAGCAATTGATTTGATAAAACGTGGACGTGTGTTTGTTAATAATTTTCAAGTATTAAAACCCTCATTTTTAGTTAAACAGACTGATTCTGTAGAAATTAAAAATGATGAAATGTATTTCGTTTCTAAAGGTGGATATAAGCTTTTAAAAATTATTAAAGAAATTAATTTAATAATTAAAAATTTTGTTATAGCTGATTTAGGGAGCTCAACAGGAGGTTTTACTGATTGTTGTTTACAATTGGGTGCTAAAAAAATTTACGCAATCGATGTTGGTGTTGACTTATTACACCCTAGTTTAAAGAAAAGTTATAAAATTATTAATTATGAAAAAACTAATGTTAAAAATTTAGATAAATCTTATTTTTTAGAAGATTTAGATTTAATTGTTGGTGATTTATCTTTCATTTCTTTAGAGCAAATTTTTCCAATAATTAAAAAAATTTCATCCCCTAAAACTTTGCTTTTATTGTTGATAAAACCCCAGTTTGAATTAGGAAAGGACGTAGCTAGTAAATATAAAGGTCTAATTCAAAATCAAAAATTACAACAATTAGCAATAAACAAAATAATCAATTTAGCAAAAAATTATGACTTTGAATGTAAACATTTAACTCCTACTGATATTTTTGATGAAAAAAAACAAAATCAAGAATATATGATATTTTTACAAAAGCATGAAAAATAA", "aa": "MNQRLDHYLKNTNQCETRSKAIDLIKRGRVFVNNFQVLKPSFLVKQTDSVEIKNDEMYFVSKGGYKLLKIIKEINLIIKNFVIADLGSSTGGFTDCCLQLGAKKIYAIDVGVDLLHPSLKKSYKIINYEKTNVKNLDKSYFLEDLDLIVGDLSFISLEQIFPIIKKISSPKTLLLLLIKPQFELGKDVASKYKGLIQNQKLQQLAINKIINLAKNYDFECKHLTPTDIFDEKKQNQEYMIFLQKHEK", "aa_hexdigest": "13d1350d7cb3675727249f79e4292af5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C68", "ncbi_nrp_id": "WP_006689051.1", "uniref100_id": "UniRef100_Q9PQ54", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C68", "RefSeq:WP_006689051.1", "UniRef:UniRef100_Q9PQ54" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ54", "uniref90_id": "UniRef90_Q9PQ54" }, "psc": { "uniref90_id": "UniRef90_Q9PQ54", "gene": "YqxC", "product": "Hemolysin", "ec_ids": [ "2.1.1.226", "2.1.1.227" ], "uniref50_id": "UniRef50_Q9PQ54", "cog_id": "COG1189", "cog_category": "J", "kegg_orthology_id": "K06442" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ54" ], "product": "Hemolysin" }, "genes": [ "yqxC" ], "id": "GPKLONFDEH_885", "locus": "GCA000006625_00864" }, { "type": "cds", "sequence": "contig_1", "start": 498443, "stop": 499525, "strand": "+", "frame": 2, "gene": "dinB", "product": "DNA polymerase IV", "db_xrefs": [ "COG:COG0389", "COG:L", "EC:2.7.7.7", "GO:0000287", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0006261", "GO:0006281", "GO:0009432", "GO:0042276", "RefSeq:WP_006689004.1", "SO:0001217", "UniParc:UPI00001297A8", "UniRef:UniRef100_Q9PQ53", "UniRef:UniRef50_Q9PQ53", "UniRef:UniRef90_Q9PQ53" ], "nt": "ATGAAAAATAATCATCAAAAAATCATCATGCATCTTGATATCGATGCTTTTTATGCTACTGTTAGTGAACTATTACATCCTGAATATAAAAATTTTCCAATTGCTGTTGGTTCATTAAATTCACGCACAGGTATTATTTCTTCACCTAACTATTTAGCACGTTCTTATGGCGTTAAAGCTGCAATGCCTATCTTTTTAGCTAAAGAATTATGTCCAAATTTAATTATTTTACCTTCTGAACACAATATTTATCAATCTTACTCAAAACATTTTTTTCAAATCATTAATAAATATACAAATAAGATTGAAATAACTTCAATTGATGAATGCTTTATTGATGCAACAGATTTAGTTAAAAAATATCATAATAATATCCGCTTGTTAGCAACAAAAATACAAAAAGAAATCAAAAACAAACTAAACCTTAGTATTTCAATTGGAATTTCATATAATAAAACAATTGCAAAAATGGCAACGGAATTAAATAAACCTTCTGGTATATCAATTATTGATGAAAATAAAATTATTAATTTAATTTATGAATTAAATATCAATAAAATTCCCTATATTGGCGAAATAAAATCACAAGAATTATATGCGATTAACATTTTTAAAATTAAAGAATTAATTGCAACTGAAAATAAACAAAAAATTTCATTAGTTTTAGGTTCAATATATCAAAATTTAGTCAATGATTTAAAAGGTTTAAGTAAAATTAAAATAATTGATGAAGATATTTATAAAACAATTTCACATTCGAAAACATTTAACGAAGATTTGAATGATTTTTATGAAATTTCTAATGAAATGAACGATTTAATTTTAACTGTTACTAATCGATTAAAAAAATGTAATTTAATGACTAATAACATCAGTATTTATATTAAATATCCTAGTTTTAAAACAAAAATTAAGCAAAAACAATTAACTTATTACACTGATGATTATCAAACCATTTTTCTAGCAATTAAAAACTTATTTAAAATAATGTATAAAGATGAAACAATACGTTTAATTGGTATCTCACTAAATAAATTAGTAAAAAAAGAAAATGTTAAAAAACAATTATTTTTATTTGATTAA", "aa": "MKNNHQKIIMHLDIDAFYATVSELLHPEYKNFPIAVGSLNSRTGIISSPNYLARSYGVKAAMPIFLAKELCPNLIILPSEHNIYQSYSKHFFQIINKYTNKIEITSIDECFIDATDLVKKYHNNIRLLATKIQKEIKNKLNLSISIGISYNKTIAKMATELNKPSGISIIDENKIINLIYELNINKIPYIGEIKSQELYAINIFKIKELIATENKQKISLVLGSIYQNLVNDLKGLSKIKIIDEDIYKTISHSKTFNEDLNDFYEISNEMNDLILTVTNRLKKCNLMTNNISIYIKYPSFKTKIKQKQLTYYTDDYQTIFLAIKNLFKIMYKDETIRLIGISLNKLVKKENVKKQLFLFD", "aa_hexdigest": "492c9c036801985e68e4c38a2a3e3918", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001297A8", "ncbi_nrp_id": "WP_006689004.1", "uniref100_id": "UniRef100_Q9PQ53", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001297A8", "RefSeq:WP_006689004.1", "UniRef:UniRef100_Q9PQ53" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ53", "uniref90_id": "UniRef90_Q9PQ53" }, "psc": { "uniref90_id": "UniRef90_Q9PQ53", "gene": "dinB", "product": "DNA polymerase IV", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q9PQ53", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0000287", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0006261", "GO:0006281", "GO:0009432", "GO:0042276" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ53" ], "product": "DNA polymerase IV" }, "genes": [ "dinB" ], "id": "GPKLONFDEH_886", "locus": "GCA000006625_00865" }, { "type": "cds", "sequence": "contig_1", "start": 499537, "stop": 499866, "strand": "-", "frame": 1, "gene": null, "product": "Holliday junction DNA helicase", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000D401D", "UniRef:UniRef100_Q9PQ52", "UniRef:UniRef50_Q9PQ52", "UniRef:UniRef90_Q9PQ52" ], "nt": "TTGATTGCTAATAATGCTAAAGGAATCCCACGATTAGCAAATCGATTGTTAAAACGAGTTGTAGATTTTAAAATTAATGGGTTTAATGATATTAAAAGTATTTTTAAAAAAATTCAAATTTATGAGTTTGGATTAGATGAACAAGATATTAATTATCTAAATGTTTTATATCGACAAGATAATGAAATTGGTTTAAAATCAATTGCACAGATTTTACGTTTAGATCAATATACAATTGAAACAAAAATAGAACCTTATTTAATTCAACATCATTTTATAAATAAGAATTTAAGAGGTCGAAAAATTACAATTGAAGGAATAGATTTTTAA", "aa": "MIANNAKGIPRLANRLLKRVVDFKINGFNDIKSIFKKIQIYEFGLDEQDINYLNVLYRQDNEIGLKSIAQILRLDQYTIETKIEPYLIQHHFINKNLRGRKITIEGIDF", "aa_hexdigest": "39bf564af26bea43d664cce28789b10b", "start_type": "TTG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI00000D401D", "uniref100_id": "UniRef100_Q9PQ52", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000D401D", "UniRef:UniRef100_Q9PQ52" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ52", "uniref90_id": "UniRef90_Q9PQ52" }, "psc": { "uniref90_id": "UniRef90_Q9PQ52", "product": "Holliday junction DNA helicase", "uniref50_id": "UniRef50_Q9PQ52" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ52" ], "product": "Holliday junction DNA helicase" }, "genes": [], "id": "GPKLONFDEH_887", "locus": "GCA000006625_00866" }, { "type": "cds", "sequence": "contig_1", "start": 499980, "stop": 500231, "strand": "+", "frame": 3, "gene": "ureE", "product": "UreE urease accessory N-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_006688998.1", "SO:0001217", "UniParc:UPI00000C1C69", "UniRef:UniRef100_A0AAP9ADA3", "UniRef:UniRef50_A0AAP9ADA3", "UniRef:UniRef90_A0AAP9ADA3" ], "nt": "ATGATTTATAAAGAAATTGTACGTAATATTATTAAACATCCATTAATTTTACCAGATGGAAAACACTATCATCTACACAAAATCTTAACTTCAAAAGATGAATTACGTAAAGGCGAAGTAACTTTAGTTGCAGAAAATGGCAAAGAATATACAGTTGATTTATCTGCCTTTGTTGATCTATTAGTTGATGGTGATTGCATCTTTGATGATAACCACTCATTAGTTGCATTATATTTTGATGATGCAAAATAA", "aa": "MIYKEIVRNIIKHPLILPDGKHYHLHKILTSKDELRKGEVTLVAENGKEYTVDLSAFVDLLVDGDCIFDDNHSLVALYFDDAK", "aa_hexdigest": "f8d5baf8bb03d0b9860833de239eec43", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "ups": { "uniparc_id": "UPI00000C1C69", "ncbi_nrp_id": "WP_006688998.1", "uniref100_id": "UniRef100_A0AAP9ADA3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C69", "RefSeq:WP_006688998.1", "UniRef:UniRef100_A0AAP9ADA3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP9ADA3", "uniref90_id": "UniRef90_A0AAP9ADA3" }, "psc": { "uniref90_id": "UniRef90_A0AAP9ADA3", "product": "UreE urease accessory N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0AAP9ADA3" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ADA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ADA3" ], "product": "UreE urease accessory N-terminal domain-containing protein" }, "genes": [ "ureE" ], "id": "GPKLONFDEH_888", "locus": "GCA000006625_00867" }, { "type": "cds", "sequence": "contig_1", "start": 500337, "stop": 500441, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATATAAAAAACGAATTTTTACAATACTTAGTAAAAATGGGTATTATTGATGATGCTAAAGAATTAGCAGATTTTAGTGTTAGTGAATTTTATTTATGA", "aa": "MQNIKNEFLQYLVKMGIIDDAKELADFSVSEFYL", "aa_hexdigest": "ad5c56f319d638f6a6c9b90137860a93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4013.586699999999, "isoelectric_point": 4.295857048034668 }, "id": "GPKLONFDEH_889", "locus": "GCA000006625_00868" }, { "type": "cds", "sequence": "contig_1", "start": 501201, "stop": 501377, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAAATGTGACATATTTTATTGATAATAAACCAAAATTTTTAGCAAATTCTATTAAGTATATTAGTTTAAGAGATCCAGAACATTATTTTGATGAAGCAACAAGAATATTTAAAAAATATTATCATCCTAATAAATTAAGTTCTAATAATAAAGATTATTTTTATTCTTGA", "aa": "MFLNVTYFIDNKPKFLANSIKYISLRDPEHYFDEATRIFKKYYHPNKLSSNNKDYFYS", "aa_hexdigest": "4021a5fc5e05f99e35b84634276e27f9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7137.003300000002, "isoelectric_point": 9.22279872894287 }, "id": "GPKLONFDEH_890", "locus": "GCA000006625_00869" }, { "type": "cds", "sequence": "contig_1", "start": 501522, "stop": 501812, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAACGTCATTTAGTTACACATTCATTAAAGAAAAAGCTATTCATTAAAACTAGCTTATTATCATTATTTTTAATTGCACCTTTGATTATTGGTTGTACAACTAATAAATCAAAATCCAATAATTCTAATAAATTGTCAAAGCAAATAGAAATCAAAGAAAATAAACAACAAGAAAAACCAAAAACTAAAGAACTATCACCAATAATTGATATTCCAAAAAATGATTATTACAAAGTTTTATATGCTAATAATATTAAAAGCTTTGTTTATGATGGTAAAGTTTGA", "aa": "MNKRHLVTHSLKKKLFIKTSLLSLFLIAPLIIGCTTNKSKSNNSNKLSKQIEIKENKQQEKPKTKELSPIIDIPKNDYYKVLYANNIKSFVYDGKV", "aa_hexdigest": "ad0de837c506e42983ebe861af32ad05", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11063.93129999999, "isoelectric_point": 9.928663063049317 }, "id": "GPKLONFDEH_891", "locus": "GCA000006625_00870" }, { "type": "cds", "sequence": "contig_1", "start": 502102, "stop": 502659, "strand": "-", "frame": 1, "gene": null, "product": "Peptidase S8/S53 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYT4", "UniRef:UniRef90_A0A2C9DYT4" ], "nt": "ATGGATAGATTTAATCAAAATTCAATATATGTAGGATCTACTAATAAAAATAATGAAAAAGTAAGTAATTTTTCAGATCGACATCACTTGAATGATGTTAAAAATAAATATGGAGATTTATTGATGCCATTTTTAGTTGCTCCTGGAGAAGGTATTTATGACACTTCAATAATTACAAAGTTTATTAAAAATAAAGGAGATAAAAATATAAATCTAGGTACTAGTTTTTCTGCGCCTATTGTCACTGGTGCGATTAGTTTATTGCAATCTTATTATATGGTTAATAACGCTAATAAAACAATTCCACTAACACATATTCGCTCAATTTTAGCTGCTTCATCAATTCCTTTAAAAAATAATGAAAATTATGATTTTTATAAAAATAGTTTTAGTCATTTATATGGTGCTGGTCATTTAGATTTTAAAAGAGCAATAGAAGCATATAAAAACGCAAAACAAACAAAAATTTTAACGAAACACAAAGATAAACAACTAGTTAAAGAAATGGATATAAGTATTGATAATGATCAAACTAATTTCAATTTTGCTTTATCATGA", "aa": "MDRFNQNSIYVGSTNKNNEKVSNFSDRHHLNDVKNKYGDLLMPFLVAPGEGIYDTSIITKFIKNKGDKNINLGTSFSAPIVTGAISLLQSYYMVNNANKTIPLTHIRSILAASSIPLKNNENYDFYKNSFSHLYGAGHLDFKRAIEAYKNAKQTKILTKHKDKQLVKEMDISIDNDQTNFNFALS", "aa_hexdigest": "17dbb506b34c7a008f559ac345d553a2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 501799, "stop": 502959, "observations": { "mutation": [ 502104, 502686 ], "stop": [ 502104, 502686 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYT4", "query_cov": 1.0, "subject_cov": 0.4111111111111111, "identity": 1.0, "score": 365.0, "evalue": 7.15e-124, "gene_start": 1, "gene_end": 185, "reference_start": 152, "reference_end": 336, "reference_sequence": "MIVKIEKVHLYSLDINAKDETSENSHIKIWKELDWLIQNDVKIINHSYGWSPNDFLNIGFYNFINGKEINDDKDFKRELKKYIDTLNKDDIVNNTINKYKFKNYIYDFISYYFDIINIFSAGNSYNDEIRYRNKNGHILEINWFDISKYSFMDRFNQNSIYVGSTNKNNEKVSNFSDRHHLNDVKNKYGDLLMPFLVAPGEGIYDTSIITKFIKNKGDKNINLGTSFSAPIVTGAISLLQSYYMVNNANKTIPLTHIRSILAASSIPLKNNENYDFYKNSFSHLYGAGHLDFKRAIEAYKNAKQTKILTKHKDKQLVKEMDISIDNDQTNFNFALSWLFKPTSNSFSKKHDYNSVYNEYKKLTYFKNDNYDIYLVKNEDLKNIDYQNFDNFGEILRTINIYRSESDSNVELIRAPNLNKGEYKILVYQKGEVRNEQTSFSYTFIKEKAIH" }, "paralog": false, "identity": 0.9948186528497409, "score": 780.0, "evalue": 6.05e-290, "description": "Internal stop codon at 502104, 502686. Nonsense mutation around 502104, 502686.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYT4", "product": "Peptidase S8/S53 domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYT4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYT4", "UniRef:UniRef50_A0A2C9DYT4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYT4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYT4" ], "product": "Peptidase S8/S53 domain-containing protein" } }, "id": "GPKLONFDEH_892", "locus": "GCA000006625_00871" }, { "type": "cds", "sequence": "contig_1", "start": 502684, "stop": 502857, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAGGATGATATTGTTAATAATACTATTAATAAATATAAATTTAAAAATTATATTTATGATTTCATATCATATTATTTTGATATTATAAATATTTTTTCAGCTGGAAATTCTTATAATGATGAAATACGATATAGAAATAAAAATGGACATATTTTAGAAATTAATTGA", "aa": "MNKDDIVNNTINKYKFKNYIYDFISYYFDIINIFSAGNSYNDEIRYRNKNGHILEIN", "aa_hexdigest": "9fff6019e4cd6a032bbeccce1b3e0456", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6950.6656, "isoelectric_point": 6.526446342468262 }, "id": "GPKLONFDEH_893", "locus": "GCA000006625_00872" }, { "type": "cds", "sequence": "contig_1", "start": 503096, "stop": 503293, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTAGTTTTTAATAACAACATTACTGTTCATGATTTAATTTCAAAAGTTTATTTTGATGCTCATTTTAATAAACCACAAGAAGAAAGTACTTCACAAAGTTTAACTTTATCATTAAATCCTAATTTTAATAATATAAATAAAGATGAATCAATTGAAATAAAGTTAATGAATTTAAATGATCGTGAAAATTGA", "aa": "MQVVFNNNITVHDLISKVYFDAHFNKPQEESTSQSLTLSLNPNFNNINKDESIEIKLMNLNDREN", "aa_hexdigest": "88e19429e599e92a06669cc6a0e8ba61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7567.3089999999975, "isoelectric_point": 4.817695808410644 }, "id": "GPKLONFDEH_894", "locus": "GCA000006625_00873" }, { "type": "cds", "sequence": "contig_1", "start": 503357, "stop": 503695, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGAATTAAGAGAAGAATTTATTGAAAATGTAGAAGATCGTTTAAGTGATGAGTTTTTTGCAGATTTTTATGATAATACTAATTTATTTGAACAAACTCCAACGCCCAATGATCATTTATTAATTAAAACCAATCCTATAAAAGATGATTTTGAAAGTTTGTTATCAAAAAAATCTGGATTACAGTATAAAATAATGCGATTAGTGTATCAACATGAACATGGTAAAGTAATTAAAACATTACCTGTTAATGCCCTAACATTCTTAAATCATGAAGATTTAAAATTTGAAGAATTGTATTCAGGGAATTATCGATTTACTGGTATTGAGTATTGA", "aa": "MLELREEFIENVEDRLSDEFFADFYDNTNLFEQTPTPNDHLLIKTNPIKDDFESLLSKKSGLQYKIMRLVYQHEHGKVIKTLPVNALTFLNHEDLKFEELYSGNYRFTGIEY", "aa_hexdigest": "d42af851231e27696b7340bba14f59ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13353.847899999988, "isoelectric_point": 4.802178764343262 }, "id": "GPKLONFDEH_895", "locus": "GCA000006625_00874" }, { "type": "cds", "sequence": "contig_1", "start": 503777, "stop": 503959, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTAATTATTTTTAATAATAAATTGGACTTAAAACAACTTGAATCATTATCAAATAGTGATTTTATTAATCAAGTAATTATTAATAAAATTAAATTTATTTTATTTAATAAAAAGATTGATCAAAAACCAATGGCTGTATTTGGTGAGTGTAAAAATTTAGTTTTAAAAAATGAATGA", "aa": "MDLIIFNNKLDLKQLESLSNSDFINQVIINKIKFILFNKKIDQKPMAVFGECKNLVLKNE", "aa_hexdigest": "f33ca871b13a106ed5efae8e519d2f67", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7012.260799999998, "isoelectric_point": 8.852492332458496 }, "id": "GPKLONFDEH_896", "locus": "GCA000006625_00875" }, { "type": "cds", "sequence": "contig_1", "start": 504020, "stop": 504316, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307CDFED", "UniRef:UniRef90_UPI00307CDFED" ], "nt": "ATGAAATTTAAAAAGAAAGCAATATTATTGTCGACAATAAGTTTAAGTTCGATGGTTTTAGGATCGTTTAGTTTGTTAGCAAAAAATAAAAGTCAACAAACAATTAGTTCGAAACTAAACCAACAAGTTAATTTGGTTGACAATCAAAATCAAAACAAAATTAGTTATAGTGTTATTGAAATTGATGAAAGCAATAAAGATTATTTTAAAGATAAATTAATTATTCGTTTGATTTTTAATACAACAAAAGCTTCAAAAACAGAAATTTTTGAGCAACATTTAAAAATTAAAACTTGA", "aa": "MKFKKKAILLSTISLSSMVLGSFSLLAKNKSQQTISSKLNQQVNLVDNQNQNKISYSVIEIDESNKDYFKDKLIIRLIFNTTKASKTEIFEQHLKIKT", "aa_hexdigest": "10a7b9d3d4d89f53ffce40c1406da1ce", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00307CDFED", "query_cov": 1.0, "subject_cov": 0.8032786885245902, "identity": 0.9490000000000001, "score": 165.0, "evalue": 3.84e-51, "valid": true, "uniref50_id": "UniRef50_UPI00307CDFED" }, "pscc": { "uniref50_id": "UniRef50_UPI00307CDFED", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307CDFED" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11239.967499999986, "isoelectric_point": 9.84453182220459 }, "id": "GPKLONFDEH_897", "locus": "GCA000006625_00876" }, { "type": "cds", "sequence": "contig_1", "start": 504656, "stop": 504970, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCACAAAAGATATTGAAAATTTTAGTGTTAAGATTTTTACGATTGTTGATAAAAAATTATATATTCCTGAAGCTAAAGAATTAATTGAAGAAAGTAAGAAAATTACTGAAACAAAAAATGGAAAAACAAATAACGAATCTAAAGTAGAAAGATTAAAAAAATTTACTAAATTTGTTGAACAAGGAATTAACAATAATAATGTGTCTTCTTATACGTTAGATCTTTATAATAAAAAACTAAAAAAACTAATTGAAGAAGTTAAAAAAATACAAAATGGAACTTCGCAAAAGATTGAAAATAATAGTAAATAA", "aa": "MFTKDIENFSVKIFTIVDKKLYIPEAKELIEESKKITETKNGKTNNESKVERLKKFTKFVEQGINNNNVSSYTLDLYNKKLKKLIEEVKKIQNGTSQKIENNSK", "aa_hexdigest": "3e38d111c07b2be74a7a75869ce5fe11", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12146.837399999988, "isoelectric_point": 9.522834587097169 }, "id": "GPKLONFDEH_898", "locus": "GCA000006625_00877" }, { "type": "cds", "sequence": "contig_1", "start": 505751, "stop": 506500, "strand": "-", "frame": 3, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2", "UniRef:UniRef90_Q9PQ15" ], "nt": "ATGATTAGTGCTATACCTTTGTTAGCAACAATAATTACAGCTTGTGCAAAAGTTGATACAAAACAAAAAGCTAAAGAAGAATTACAATCAAGTTTAAATAAAGCTGTTTTATTAAAAGAAATAGTTGATCAATCATCTTATGAAGATTTTAAAAAGATATATAATCAAACTCTTAAATCAAATTTAGAAAACATTAATAAATTATCAGAGCAAAAATTAAAACAAGAACAACAAACAATTAAAAATTTAATTACACAAGTTAGTGTTTTAAATGAATTAGATTCAATCAAAAACATGAGTGATTTAATAGATGTTGTTGAATTAGAAAACACTAAAAAACAATTACAAGATTTTTTAAATCAATATGAAAAATTACTTAATCAAGCAAGTGTTGATACTACAATAATTGATAAACTAAAATCTGATGCTCATCAAAAAAGATTGCACACTAAAGCTTTAATTCAAAAAACAATTTTAGATTTAGCTAATAAAATTAAAAATTTAATTAAAACATCTGGATCAAATAAAAATATTGTCGAGATTGAAAGTGATATTCAAAAATTAGTTAATAATAACACAAAAGCTAATTTTAAAAAACTATATGAAGATTTTAAATTAATTTACACTAAATTAAATGATATTAATGATTTAATTAACAAAGAACTAAAAGCTTTACCTTTTATTAAAAAATTAGATGATATTGTTAATGTTATTAATACTATTTACCAACAAAATTATAGTTTTTTATGA", "aa": "MISAIPLLATIITACAKVDTKQKAKEELQSSLNKAVLLKEIVDQSSYEDFKKIYNQTLKSNLENINKLSEQKLKQEQQTIKNLITQVSVLNELDSIKNMSDLIDVVELENTKKQLQDFLNQYEKLLNQASVDTTIIDKLKSDAHQKRLHTKALIQKTILDLANKIKNLIKTSGSNKNIVEIESDIQKLVNNNTKANFKKLYEDFKLIYTKLNDINDLINKELKALPFIKKLDDIVNVINTIYQQNYSFL", "aa_hexdigest": "61dc4a1ee20cfe9126ea8fd9cb7ce787", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 505448, "stop": 506542, "observations": { "mutation": [ 505753, 506515, 506521 ], "stop": [ 505753, 506515, 506521 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ15", "query_cov": 1.0, "subject_cov": 0.4042207792207792, "identity": 0.9640000000000001, "score": 437.0, "evalue": 8.44e-149, "gene_start": 1, "gene_end": 249, "reference_start": 15, "reference_end": 263, "reference_sequence": "MKHKKYKSIWLKLSMISAIPLLATIITACAKVDTKQKAKEELQSSLNKAVLLKEIVDQSSYEDFKKIYNQTLKSNLENINKLSEQKLKQEQQTIKNLITQVSVLNELDSIKNMSDLIDVVELENTKKQLQDFLNQYEKLLNQASVDTTIIDKLKSDAHQKRLDTKALIQKTILDLANKIKSLIKTSGLNKNIVELENDIQKLVNNDTKANFKKLYEDFKLIYTKLNDINDLINKELKALPFIKKLDDIVNVINTIYEQNHHFLWFDQTKLTNLRQILIKNINHVLNAKKTLEIKEIKTLTNQLLFDLQNYLEGLKMIALESENSVNKLISIIESLQPDDYNPYYSIEKQENKQKDENNSQATYNGLEHQIKSLKSIINLFDFNIANLSIDAIYNLNDELIKKFQKANSQINEVVIQIKQTILSNFKQTYLFKDFIKHLKEFNIELNNENDPIKKANRIQTIQEVLKSYEKDLFLNTKINLKLAPNEVLTVNNLEKIDLTYASDLLQKPQELIAYLELNNNQEREFSKELENLSVRVFTIIDKKLYIPKAKELINESKKIIQTKDEYTNNKTKLQRLEKFTSFVEQGISNENVSSYTLNLYIQRLTKLIQEIKKPQH" }, "paralog": false, "identity": 0.8901098901098901, "score": 618.0, "evalue": 3.35e-222, "description": "Internal stop codon at 505753, 506515, 506521. Nonsense mutation around 505753, 506515, 506521.", "psc": { "uniref90_id": "UniRef90_Q9PQ15", "product": "Unique hypothetical", "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ15", "UniRef:UniRef50_A0AAC9X6L2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_899", "locus": "GCA000006625_00878" }, { "type": "cds", "sequence": "contig_1", "start": 506698, "stop": 506874, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C6E", "UniRef:UniRef100_A0A2C9DZ21", "UniRef:UniRef50_A0A2C9DZ21", "UniRef:UniRef90_A0A2C9DZ21" ], "nt": "ATGAAAAATCAAAAAGATTTTTATTTTTATGGAGCTAGTTCAAATGCTGTTTTAAATAAAAAAGTTAATTTTGATCTTTTAAAAAACACAAGTATTAACAAAACTCTAGAACAACTAGTTGCGAATAAAGTAAAATTTATTATTGACATTAAACATTTATTAGATTTGTTACTTTAA", "aa": "MKNQKDFYFYGASSNAVLNKKVNFDLLKNTSINKTLEQLVANKVKFIIDIKHLLDLLL", "aa_hexdigest": "fa6ab7141dfc7c478e6bb105e8709dbb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C6E", "uniref100_id": "UniRef100_A0A2C9DZ21", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C6E", "UniRef:UniRef100_A0A2C9DZ21" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DZ21", "uniref90_id": "UniRef90_A0A2C9DZ21" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DZ21", "uniref50_id": "UniRef50_A0A2C9DZ21" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DZ21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DZ21" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6713.838199999998, "isoelectric_point": 9.574602699279783 }, "id": "GPKLONFDEH_900", "locus": "GCA000006625_00879" }, { "type": "cds", "sequence": "contig_1", "start": 507066, "stop": 507419, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DZ03", "UniRef:UniRef90_A0A2C9DZ03" ], "nt": "ATGAAAAAAGATAATCATGAATTACTTTTTAAATTACAAAATAATAAAAATTTACCTAATCAAAATGAAATTTTTATTCCTATTGAAAAATATAAAAAATGTGAAAACATGTTAGATTTAAACATTTGTGATAAGTATGCATATAAAGATCTTAAAGAACAAAATCACTATTTTGATTCTTCAACTGAATCTAAATTTGCAAATCGTTTTCTTTCATTATCAAATGATAATGATAAAATTAAAAATAATGTTGAACTTTGGACTAAAAATCCTGTTTTTCATGGATTAAAATATCAATATTTTAATGAAGATGGTCAAATAAGTAATTCATACCCTGATTTTATTTTAAAATAA", "aa": "MKKDNHELLFKLQNNKNLPNQNEIFIPIEKYKKCENMLDLNICDKYAYKDLKEQNHYFDSSTESKFANRFLSLSNDNDKIKNNVELWTKNPVFHGLKYQYFNEDGQISNSYPDFILK", "aa_hexdigest": "500253df46e34826d71ed353602b0ca8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2C9DZ03", "query_cov": 1.0, "subject_cov": 0.8297872340425532, "identity": 0.991, "score": 240.0, "evalue": 2.9e-80, "valid": true, "uniref50_id": "UniRef50_A0A2C9DZ03" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DZ03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DZ03" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14064.679499999984, "isoelectric_point": 6.821668052673339 }, "id": "GPKLONFDEH_901", "locus": "GCA000006625_00880" }, { "type": "cds", "sequence": "contig_1", "start": 508084, "stop": 508224, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGGCGGTACAGTTGGAAAAAATATAATTATAAAAAAAGCTAATGATTATTATTTAAATCAAAGAGTTGCTAGAATCAGAACATTTAATGTAAATTATAATTATATTTATTATTTAATAAATACTACTTATATATAA", "aa": "MTGGTVGKNIIIKKANDYYLNQRVARIRTFNVNYNYIYYLINTTYI", "aa_hexdigest": "9860fd21a0d3e752150158eb142fef2a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5468.2469, "isoelectric_point": 9.820614051818847 }, "id": "GPKLONFDEH_902", "locus": "GCA000006625_00881" }, { "type": "cds", "sequence": "contig_1", "start": 508300, "stop": 508674, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction enzyme S protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniParc:UPI00000D401F", "UniRef:UniRef100_Q9PQ43", "UniRef:UniRef50_Q9PQ43", "UniRef:UniRef90_Q9PQ43" ], "nt": "TTGCCACCATTAGATGAACAACAACGAATAGTTGACAAAATTAATCTATTAGAATTTTTTATTAAACAATATGATGAAATAGAACAAAAATTATCGAAGCTTGAAAATGAATTTCCAGAAAAGCTAAAAAAATCGGTTTTACAATATGCTATGCAAGGCAAATTAATTAAACAAGATCCAAATGATGATTCTATAAAAGATTTATTAAAACAAATTCATAAAGAAAAACAAAAATTATACAAAGAGGGTAAACTTAAAAAGAAGGATTTAGAAGAATCTATAATTTATAAAAGTGATGATAAGTCGTATTATGAGAATATAGGTAATAATGAGCCTAAAAAGCTTGAAAATCTACCATTTAATATAAATGTATAA", "aa": "MPPLDEQQRIVDKINLLEFFIKQYDEIEQKLSKLENEFPEKLKKSVLQYAMQGKLIKQDPNDDSIKDLLKQIHKEKQKLYKEGKLKKKDLEESIIYKSDDKSYYENIGNNEPKKLENLPFNINV", "aa_hexdigest": "205d68209b8b74e32c6c0ff57cfc8d0c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000D401F", "uniref100_id": "UniRef100_Q9PQ43", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000D401F", "UniRef:UniRef100_Q9PQ43" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ43", "uniref90_id": "UniRef90_Q9PQ43" }, "psc": { "uniref90_id": "UniRef90_Q9PQ43", "product": "Type I restriction enzyme S protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q9PQ43", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ43" ], "product": "Type I restriction enzyme S protein" }, "genes": [], "id": "GPKLONFDEH_903", "locus": "GCA000006625_00882" }, { "type": "cds", "sequence": "contig_1", "start": 508750, "stop": 509688, "strand": "-", "frame": 1, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.-", "EC:3.6.4.12", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "RefSeq:WP_006689015.1", "SO:0001217", "UniParc:UPI000013539B", "UniRef:UniRef100_Q9PQ42", "UniRef:UniRef50_Q9PQ42", "UniRef:UniRef90_Q9PQ42" ], "nt": "ATGAAAACTAATTATGAGTTTCGTCCACAATATCTAAAAGATTTTATTGGTAAAGAACAATTAAAAAACAATTTAAAAGTTTATTTAACTGCTAGTAAACGATTAGAAAATAGTTTTGATCATACATTATTACATGGATTATCAGGAACAGGGAAAACAACATTAGCACTTATAATTGCTAATGAAATGAATGTTAATTGTCACATAACACAAGGGAACTTGTTAAATAAACCTATTGATATTATTAATTTATTATCTTTAATTAAAGAAAATGACGTTGTTTTTATTGATGAAATTCACGCTTGTGGTTTAGGTGCATTTGAAACACTTTATTCTGTATTAGAAGATTTTTGTATTGATATTAATATAGGTAAAGATTTTAATTCAAAAATGACGAGGTTAAAGATTCCTCATTTTACACTTATTGGTGCAACAACAATTTTTGGAAAAATCCCAAAATCTTTAGAAGAACGCTTCGGACACATCTTTCATTTAAATGAATATGAACCATCAGAAATTAGTGCAATTATTTTAAAGAATAATCAGATGCATTTTCAGATTGATTTAAACGAAGAAGAAATTGATTTGATTGCTAATAATGCTAAAGGAATCCCACGATTAGCAAATAGATTATTAAAACGAGTTGTAGATTTTAAAATTAATGGGTTTAATGATATTAAAAATATTTTTAAAAAAATTCAAATTTATGAGTTTGGATTAGATGAACAAGATATTAATTATCTAAATGTTTTATATCGACAAGATAATGAAATTGGTTTAAAATCAATTGCACAGATTTTACGTTTAGATCAATATACAATTGAAACAAAAATAGAACCTTATTTAATTCAACATCATTTTATAAATAAGAATTTAAGAGGTCGAAAAATTACAATTGAAGGAATAGATTTTTTAAAAAACAATCAACTAATCAAATAA", "aa": "MKTNYEFRPQYLKDFIGKEQLKNNLKVYLTASKRLENSFDHTLLHGLSGTGKTTLALIIANEMNVNCHITQGNLLNKPIDIINLLSLIKENDVVFIDEIHACGLGAFETLYSVLEDFCIDINIGKDFNSKMTRLKIPHFTLIGATTIFGKIPKSLEERFGHIFHLNEYEPSEISAIILKNNQMHFQIDLNEEEIDLIANNAKGIPRLANRLLKRVVDFKINGFNDIKNIFKKIQIYEFGLDEQDINYLNVLYRQDNEIGLKSIAQILRLDQYTIETKIEPYLIQHHFINKNLRGRKITIEGIDFLKNNQLIK", "aa_hexdigest": "9e99b74b4e60d37eca5a020ddfee84cd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013539B", "ncbi_nrp_id": "WP_006689015.1", "uniref100_id": "UniRef100_Q9PQ42", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013539B", "RefSeq:WP_006689015.1", "UniRef:UniRef100_Q9PQ42" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ42", "uniref90_id": "UniRef90_Q9PQ42" }, "psc": { "uniref90_id": "UniRef90_Q9PQ42", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.-", "3.6.4.12" ], "uniref50_id": "UniRef50_Q9PQ42", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ42" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "GPKLONFDEH_904", "locus": "GCA000006625_00883" }, { "type": "cds", "sequence": "contig_1", "start": 509837, "stop": 510265, "strand": "-", "frame": 3, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518", "SO:0001217", "UniRef:UniRef50_Q9PQ41", "UniRef:UniRef90_Q9PQ41" ], "nt": "ATGAACTATTTAATTTTTAAAGTTATTTATGCAAATGCAAATATTGTTGTTGGCGAACATAATTTTATTGGTTATCAAATACGTATCCCAAAAGATTATGAATTAGAAATTAATAAATTTTGCAAATTATATTTGTATGAATATATTAGCATAATGCCTAATAAAAATTTAATTATTAAAGATTTATATGGTTTTCGTACTTATAATGAACGGTTATTATTTATAGACTTGATTAGTATTAATTCAATTGGCCCAAAAACAGCTATTAATATTCTAAAATATGATATTGATACAATTATTGATGCAATTGCTACTAAGAATATAGATTATTTAATAACAATTAAAGGAATTAACCAACGAAATGCTAATTTGATTTGTGATCAATTAAATTATAAATACATAAATAAAGTAAATGATAAAAATAACTGA", "aa": "MNYLIFKVIYANANIVVGEHNFIGYQIRIPKDYELEINKFCKLYLYEYISIMPNKNLIIKDLYGFRTYNERLLFIDLISINSIGPKTAINILKYDIDTIIDAIATKNIDYLITIKGINQRNANLICDQLNYKYINKVNDKNN", "aa_hexdigest": "69e71fc2f0af406a6f8bf6ecc247edd8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "ruvA" ], "truncated": "5-prime", "pseudogene": { "start": 509678, "stop": 510265, "observations": { "mutation": [ 509839 ], "stop": [ 509839 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ41", "query_cov": 1.0, "subject_cov": 0.7282051282051282, "identity": 1.0, "score": 281.0, "evalue": 4.99e-95, "gene_start": 1, "gene_end": 142, "reference_start": 1, "reference_end": 142, "reference_sequence": "MNYLIFKVIYANANIVVGEHNFIGYQIRIPKDYELEINKFCKLYLYEYISIMPNKNLIIKDLYGFRTYNERLLFIDLISINSIGPKTAINILKYDIDTIIDAIATKNIDYLITIKGINQRNANLICDQLNYKYINKVNDKNNWAKELSIGLENLGYTKKDIEYAITKVKINSQQDIDISEIISSAIKEISLRHEN" }, "paralog": false, "identity": 0.9948717948717949, "score": 384.0, "evalue": 4.82e-138, "description": "Internal stop codon at 509839. Nonsense mutation around 509839.", "psc": { "uniref90_id": "UniRef90_Q9PQ41", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q9PQ41", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ41", "EC:3.6.4.12", "UniRef:UniRef50_Q9PQ41", "COG:COG0632", "COG:L", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ41" ], "product": "Holliday junction branch migration complex subunit RuvA" } }, "id": "GPKLONFDEH_905", "locus": "GCA000006625_00884" }, { "type": "cds", "sequence": "contig_1", "start": 510307, "stop": 510417, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTATTTATTTAGGTAGTTTAATTGGGATGTTTGGGTTTGGTTATTTTTTAATTTTTGATTTATATAAATTTATTAAAAAAGCTAAAGTTGATAAAAAAAGATAA", "aa": "MFSIYLGSLIGMFGFGYFLIFDLYKFIKKAKVDKKR", "aa_hexdigest": "c51d02e559d8b40f1a87c787efd16cb0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4286.193500000002, "isoelectric_point": 9.901779747009275 }, "id": "GPKLONFDEH_906", "locus": "GCA000006625_00885" }, { "type": "cds", "sequence": "contig_1", "start": 510460, "stop": 510729, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTATTTTAAAACACTGCGGGTTTCATTATTGGCTTTTGTTATTGGAGTAGTCTATAACATTGGTATTTATGCACTAATGTATATTTCATATCGTTTAGTAGAACCAAAACCACATAACATCTTTTTTGATTTATTTAATTATACAAATATTGCTGTCACAGCAACTAATTTTGTCCCTTTGCCTGGATCTGAGGGTTCAATTCAATTTATGTTACGTGTTTTTTTAACAAATGATCAAAATCAAATTCGTTACGCAATGAATTGA", "aa": "MYYFKTLRVSLLAFVIGVVYNIGIYALMYISYRLVEPKPHNIFFDLFNYTNIAVTATNFVPLPGSEGSIQFMLRVFLTNDQNQIRYAMN", "aa_hexdigest": "c2125aedb287350c025bb165cbf9437b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10372.024599999995, "isoelectric_point": 9.048347282409669 }, "id": "GPKLONFDEH_907", "locus": "GCA000006625_00886" }, { "type": "cds", "sequence": "contig_1", "start": 510817, "stop": 511023, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCCAAGTTATAGTAACTGTACCATCTTTTATTTATATTAATTATGAATTAATAACTTATGCAGTTAATAATAAAACAAGTTTACCCTTTTATTATTATTTGGCTGTTGTTGGTATAATTGCTGATTTATGTATGACAGCTATTTATTTTGTTTTAGGCTTCTCTAAAAAATTGCATTTTCGTTCAGTTTTAATTTATAATTGA", "aa": "MGQVIVTVPSFIYINYELITYAVNNKTSLPFYYYLAVVGIIADLCMTAIYFVLGFSKKLHFRSVLIYN", "aa_hexdigest": "51d6bd382ca0aeb17730957b395ff423", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7824.220399999999, "isoelectric_point": 8.788281822204592 }, "id": "GPKLONFDEH_908", "locus": "GCA000006625_00887" }, { "type": "cds", "sequence": "contig_1", "start": 511919, "stop": 512320, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAACCACAAATTTTACAAGATTGCAATGATCTATCATCAGCTCCAGTTAATAACCAAGAAGATGATATTGTTAACGAACATGAATCTACACCTATTGAACCATTACATGAAGAAGTTGAACCTGAGCCTATAAAGAACGAAAATCAAAGCAATGAAGAATTTATACAGGAGAATATTGTAGCTCCAGTTGATGAAGTTGATAATTCAGTAAAAGAACATACACCTTTTACTCCTGTTTCTTTTGAATTTGAAGAACCTGTTTTTGAAAATATTTATTCAAACCAAAAAGTGTATGCTGAAAATTTATTTTTAGAATCTTTATTTTCTGAAAAAGAAAGTTTAATTAATGATGATCAAAATGATTTTAGTTGGTTATCAAAGTATGTAAGTGTTTGA", "aa": "MDEPQILQDCNDLSSAPVNNQEDDIVNEHESTPIEPLHEEVEPEPIKNENQSNEEFIQENIVAPVDEVDNSVKEHTPFTPVSFEFEEPVFENIYSNQKVYAENLFLESLFSEKESLINDDQNDFSWLSKYVSV", "aa_hexdigest": "3c7a16924b1207acf9a7d939e8c6f62f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15392.38619999999, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_909", "locus": "GCA000006625_00888" }, { "type": "cds", "sequence": "contig_1", "start": 512396, "stop": 512566, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTATTTAGTAAGAAAAATAAATTAAATCAAAAGTTAACTGAAGATAAAAATATAAATATTACTGATGTTTTAGATAATAACAAAGATATTTATCAATTATATGATGAAACTATTGTTACTAAAAAAGTTAAAGTAAGATGCAAAAATTGTGGCGTTTTTATGTGA", "aa": "MGLFSKKNKLNQKLTEDKNINITDVLDNNKDIYQLYDETIVTKKVKVRCKNCGVFM", "aa_hexdigest": "f6db3a32dd4346950941d6a8792743d3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6553.606300000001, "isoelectric_point": 9.230728340148925 }, "id": "GPKLONFDEH_910", "locus": "GCA000006625_00889" }, { "type": "cds", "sequence": "contig_1", "start": 512663, "stop": 513430, "strand": "-", "frame": 3, "gene": "proS", "product": "proline--tRNA ligase", "db_xrefs": [ "COG:COG0442", "COG:J", "EC:6.1.1.15", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101", "SO:0001217", "UniRef:UniRef50_Q9PQ38", "UniRef:UniRef90_Q9PQ38" ], "nt": "ATGATGCAAGATGGCCAAGCATTACAATCTGCAACAAGTCATTATTTAGGGCAGTTTTTTGCAAAAGCTTATGATATTAAGTTTCAAGGTCAAGATAATCAAATGCATTATGTACATCAAATGTCTGCTGGATTATCAACACGAATTATTGGAGCATTAATTATGGTTCATGCTGATGATCAGGGTTTGATTTTGCCCCCTGATATTGCTTTTAATCAAATTGCTATTTTAACGATTTTTTCTAATAAAAATCCCCAATTATTAAAAATTAGTCAACAGATTTGTGAACAATTAAATAATTATCGTTTGTTTGAAGATCATAGTGATAAAGGAATTGGTTATAAATTAGCACAACAAGAAATTGAGGGAACGCCTATTTGTATATTAGTTGGTGCTAAAGAATTAGTTAATCAACAAGTTGTTATTGTGCGTCGTGATACTCATGAAAAAATTAATGTTGATTTAACTGATTTGAAACTAATCATCCCAAAATTATTAGCAGATATTAAAAGAAATATTTATGAAAAGGCAAAAAAAGATTTAGAGGATTCGATTGTTTTTGTAAATAATATCGAGGAATTAAAACAAGTTATTGCACAAAATAAAATGGCTAAGGCATTCTTTGATGGTACAAAAGAAGATGATGAACAAATTAAATTATTAACTAATGCTTCAACACGTTGTATTTTTGATGAAACTCAAAACGGGCAGTGTTTTTATACAAATAAAAAAACAAACAAATTGACTTTATTTGCACGTGCTTATTAG", "aa": "MMQDGQALQSATSHYLGQFFAKAYDIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILTIFSNKNPQLLKISQQICEQLNNYRLFEDHSDKGIGYKLAQQEIEGTPICILVGAKELVNQQVVIVRRDTHEKINVDLTDLKLIIPKLLADIKRNIYEKAKKDLEDSIVFVNNIEELKQVIAQNKMAKAFFDGTKEDDEQIKLLTNASTRCIFDETQNGQCFYTNKKTNKLTLFARAY", "aa_hexdigest": "60b7614d17589eddd7fb4b6466d73018", "start_type": "ATG", "rbs_motif": null, "genes": [ "proS" ], "truncated": "3-prime", "pseudogene": { "start": 512663, "stop": 513730, "observations": { "mutation": [ 513475, 513607 ], "stop": [ 513475, 513607 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ38", "query_cov": 1.0, "subject_cov": 0.5402542372881356, "identity": 1.0, "score": 503.0, "evalue": 1.58e-176, "gene_start": 1, "gene_end": 255, "reference_start": 218, "reference_end": 472, "reference_sequence": "MSKKLEKITTRKENFADWYTSIVNNAKLIQYTDIKGMMVFQPNAWAIWEAIKNQIDVEFKKHGVRNLAMPTLIPLSEFQKEKDHIEGFAPELFMVNQIGDKKLDNSYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFHWQELHAIFASENEADSFAKVILDVYTDFVQNYLCIPVIKGLKTPWERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKAYDIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILTIFSNKNPQLLKISQQICEQLNNYRLFEDHSDKGIGYKLAQQEIEGTPICILVGAKELVNQQVVIVRRDTHEKINVDLTDLKLIIPKLLADIKRNIYEKAKKDLEDSIVFVNNIEELKQVIAQNKMAKAFFDGTKEDDEQIKLLTNASTRCIFDETQNGQCFYTNKKTNKLTLFARAY" }, "paralog": false, "identity": 0.9943661971830986, "score": 704.0, "evalue": 2.24e-258, "description": "Internal stop codon at 513475, 513607. Nonsense mutation around 513475, 513607.", "psc": { "uniref90_id": "UniRef90_Q9PQ38", "gene": "proS", "product": "proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_Q9PQ38", "cog_id": "COG0442", "cog_category": "J", "go_ids": [ "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ38", "EC:6.1.1.15", "UniRef:UniRef50_Q9PQ38", "COG:COG0442", "COG:J", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ38" ], "product": "Proline--tRNA ligase" } }, "id": "GPKLONFDEH_911", "locus": "GCA000006625_00890" }, { "type": "cds", "sequence": "contig_1", "start": 513605, "stop": 513877, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGCCAACTTTAATTCCTTTAAGTGAATTTCAAAAAGAAAAAGATCATATTGAAGGTTTTGCTCCTGAATTATTTATGGTTAATCAAATTGGTGATAAAAAATTAGATAATTCTTATGCAATACGTCCTACGAGTGAAATTTTATTTTGTAATTATTTTAAAAATATTGTTAATAGTTATAATGATTTACCGATTAAAAATAATCAATGGTGTTCAGTAATGCGTGCTGAAAAAACAACACGTCCATTTCTTCGAAATGCTGAGTTTCATTGA", "aa": "MPTLIPLSEFQKEKDHIEGFAPELFMVNQIGDKKLDNSYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFH", "aa_hexdigest": "cdbb67b52f171035ed67227a914633a6", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 59, "aa_cov": 0.6333333333333333, "hmm_cov": 0.9830508474576272, "evalue": 6.659839308717688e-11, "score": 38.15842056274414, "start": 34, "stop": 90 } ], "seq_stats": { "molecular_weight": 10598.02819999999, "isoelectric_point": 6.715720176696778 }, "id": "GPKLONFDEH_912", "locus": "GCA000006625_00891" }, { "type": "cds", "sequence": "contig_1", "start": 514107, "stop": 514574, "strand": "-", "frame": 2, "gene": "iscU", "product": "Iron-sulfur cluster assembly scaffold protein", "db_xrefs": [ "COG:COG0822", "COG:O", "RefSeq:WP_006688443.1", "SO:0001217", "UniParc:UPI00000C1C73", "UniRef:UniRef100_A0AAC9T0G2", "UniRef:UniRef50_A0A084F069", "UniRef:UniRef90_A0AAC9T0G2" ], "nt": "ATGGCATCTTATAATATCAATGACAATCTTTTATTACGTAAGATTATCATGCAACACTATGAACACCCTAAAAATAAAGTTTGTTTTGTACAAGATGAAATAAATTATTTGTCATATCATAATACAACAGAAGGATGTGGTGATGACATTACAGTTTATGTTAAATTAAATCATCAAAAAATAATTGATGTCGTTTTTTTAGGTACAGGCTGTGCAATTTCCATATCATCCACTGATATTATTTGTGAACTTGTAAAAAATCTAGATCTTAATAATGCGTTATTATTAATTAATAATTATTTAAATATGATTGAAGGGCTAGATTACAATAAAGATATTATGCAAGAATTAATTGCTTTTTATAATGTAAAAAAACAAATGAATCGTATTCGTTGTGCCCGTATTGGCGCAAGTGCTTTAAAAATGTGTTTAAAGCAATTTACAGATAAACACAAAACAATTGTTTAA", "aa": "MASYNINDNLLLRKIIMQHYEHPKNKVCFVQDEINYLSYHNTTEGCGDDITVYVKLNHQKIIDVVFLGTGCAISISSTDIICELVKNLDLNNALLLINNYLNMIEGLDYNKDIMQELIAFYNVKKQMNRIRCARIGASALKMCLKQFTDKHKTIV", "aa_hexdigest": "65a536e1721bf36edd2f85f68b1c6875", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1C73", "ncbi_nrp_id": "WP_006688443.1", "uniref100_id": "UniRef100_A0AAC9T0G2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C73", "RefSeq:WP_006688443.1", "UniRef:UniRef100_A0AAC9T0G2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0G2", "uniref90_id": "UniRef90_A0AAC9T0G2" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0G2", "gene": "IscU", "product": "Iron-sulfur cluster assembly scaffold protein", "uniref50_id": "UniRef50_A0A084F069", "cog_id": "COG0822", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_A0A084F069", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F069" ], "product": "Nitrogen fixation protein NifU" }, "genes": [ "iscU" ], "id": "GPKLONFDEH_913", "locus": "GCA000006625_00892" }, { "type": "cds", "sequence": "contig_1", "start": 514561, "stop": 515301, "strand": "-", "frame": 1, "gene": "csd", "product": "putative cysteine desulfurase", "db_xrefs": [ "COG:COG0520", "COG:E", "EC:2.8.1.7", "GO:0031071", "SO:0001217", "UniRef:UniRef50_Q9PQ36", "UniRef:UniRef90_Q9PQ36" ], "nt": "GTGGTTTCGTTTGCTTCTGGTGGTAATTTAATTGGTAATGTTTTAGATGAAAATCTTATTATTAAAAATATTAAAAAAATTAAACCAGATGTATTAGTGTGCGTTGATGCAACTCAAAGTATTCAACACCGTATGTTTGATGTGAAAAAATGCAAAAGTGATTTTATGGTCTTTTCAGCACATAAATTATTAGGACCTACAGGAATTGGTGTTGCTTATATTAAAAATGACTTGATTAAAAAAATGCAACCATTAAAATATGGTGGTGGAATGAATTTTAGCATTGATTTAAATAGTTATCAGCTCTATGATAATTACATGAAATTTGAGGGTGGAACTCCGCATGTTGCTGGTTTTTATGGTTTTAATGCTGCTTTAAAATTTTTAATGGATATAGGTTATAATAAAATTCATGAACATGAATTAAAAATTACTAAATATGCACGTGACCAATTAGCTTTAATTCCTGAAATTAAAACATATGTGCAAGATGCTACTTCAAGTACTATAACTTTTAGTTATAATGGTGTTTTTTGCCAAGATTTTGCTAATTATTTAGGATCAAAAAATATTATTGTGCGAAGTGGATTATCATGTGCAAAAATTATTAATAACGTGATTCAAACCGAATGTGCTATTCGTGCATCTTTTTACATATATAATGATTTTAATGATGTTGATAGATTAATTAAGGCAATTAAAGAATATCAAAAAGGAGATGAATTAAATGGCATCTTATAA", "aa": "MVSFASGGNLIGNVLDENLIIKNIKKIKPDVLVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLGPTGIGVAYIKNDLIKKMQPLKYGGGMNFSIDLNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITKYARDQLALIPEIKTYVQDATSSTITFSYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNVIQTECAIRASFYIYNDFNDVDRLIKAIKEYQKGDELNGIL", "aa_hexdigest": "543d0743c3454f6bdabaafd0a954cc03", "start_type": "GTG", "rbs_motif": null, "genes": [ "csd" ], "truncated": "3-prime", "pseudogene": { "start": 514561, "stop": 515601, "observations": { "mutation": [ 515409 ], "stop": [ 515409 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ36", "query_cov": 1.0, "subject_cov": 0.6119402985074627, "identity": 0.996, "score": 494.0, "evalue": 1.49e-174, "gene_start": 1, "gene_end": 246, "reference_start": 157, "reference_end": 402, "reference_sequence": "MNDYKQFFPWFKNNKDVIYLDSSATSLKPQVVIDAIVDYYTKYSTNPHNTDSNFTFHTHEIMNETRANVAKFINANFDEIIFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLPWYKLRDDLGVKIIFANQKNQFPQLSDFLKVISPKTKVVSFASGGNLIGNVLDENLIIKNIKKIKPDVLVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLGPTGIGVAYIKNDLIKKMQPLKYGGGMNFSIDLNSYQLYDNYMKFEGGTPHVAGFYGFNAALKFLMDIGYNKIHEHELKITKYARDQLALIPEIKTYVQDATSSTITFSYNGVFCQDFANYLGSKNIIVRSGLSCAKIINNVIQTECAIRASFYIYNDFNDVDRLIKAIKEYQKGDELNGIL" }, "paralog": false, "identity": 0.9971098265895953, "score": 691.0, "evalue": 2.5e-254, "description": "Internal stop codon at 515409. Nonsense mutation around 515409.", "psc": { "uniref90_id": "UniRef90_Q9PQ36", "gene": "csd", "product": "Probable cysteine desulfurase", "ec_ids": [ "2.8.1.7" ], "uniref50_id": "UniRef50_Q9PQ36", "cog_id": "COG0520", "cog_category": "E", "go_ids": [ "GO:0031071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ36", "EC:2.8.1.7", "UniRef:UniRef50_Q9PQ36", "COG:COG0520", "COG:E", "GO:0031071" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ36" ], "product": "Probable cysteine desulfurase" } }, "id": "GPKLONFDEH_914", "locus": "GCA000006625_00893" }, { "type": "cds", "sequence": "contig_1", "start": 515407, "stop": 515586, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGAATGAAACAAGAGCAAATGTTGCCAAATTCATCAATGCTAATTTTGATGAAATCATTTTTACAAGTGGTGCTACAGAATCTCTAAATTTGATTGCAAACGGTTTAAGACCTCATTTAAAAAAGGGTGATGAAATTATTTTAACTTATATTGAACATGCTTCTAATTTATTACCATGA", "aa": "MNETRANVAKFINANFDEIIFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLP", "aa_hexdigest": "8a627341db8848e9d6a827f9c621fb2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 60, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 5.80178404202445e-23, "score": 76.99227142333984, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 6540.372399999998, "isoelectric_point": 5.480040550231934 }, "id": "GPKLONFDEH_915", "locus": "GCA000006625_00894" }, { "type": "cds", "sequence": "contig_1", "start": 515981, "stop": 516232, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTAAAAAAATTCTTTTAACAAGCACATTAAGTGTTTTAGGGGTTATTTCAATTGCAAGTTTAGCTACAAATTGTTCACAAACGAAAACAAAAATTAACCAGTATAAAATAACTATAAATAAATTAGATAGTCTAAATTTAACAAGTAATTTAACTACAAAACAAGCTTTAAAAGATGTTTTATTCTCAAAAGAAGGACAAAATGAATTTTTATTATCAAATGTTAAAAAAGTGGTTTATGATTGA", "aa": "MKFKKILLTSTLSVLGVISIASLATNCSQTKTKINQYKITINKLDSLNLTSNLTTKQALKDVLFSKEGQNEFLLSNVKKVVYD", "aa_hexdigest": "27b7cee8f791b52476c58fecb614c1c2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9238.762299999997, "isoelectric_point": 9.76465549468994 }, "id": "GPKLONFDEH_916", "locus": "GCA000006625_00895" }, { "type": "cds", "sequence": "contig_1", "start": 516539, "stop": 516796, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAGAATGAAAGTACTTTTAAATCAACTAAAATTGGAGATAGAAACAAAATTGTTGCTAAGACAAATAATTTTTTACAAAAAGGATACGCTAATTTATTTAATTTCTTAATTGATGATTATATTAAATATAACCTGCCTTTATCATTTGCCAACATTGAATTTAAATATGATGATTTAAAAAATCAAAACATTTATAATAAAGATTTTTTTACAAAAATACCAACTTCTGGCACATCACCTAATTTTCCTTGA", "aa": "MLENESTFKSTKIGDRNKIVAKTNNFLQKGYANLFNFLIDDYIKYNLPLSFANIEFKYDDLKNQNIYNKDFFTKIPTSGTSPNFP", "aa_hexdigest": "0b921d1e937cc6a1668754bfd380173c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9929.101899999989, "isoelectric_point": 8.859906196594238 }, "id": "GPKLONFDEH_917", "locus": "GCA000006625_00896" }, { "type": "cds", "sequence": "contig_1", "start": 517325, "stop": 518185, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACGCTCTATTAACAGAGTTAAAAAATGATTTTTTATACAATGGTGCACTATACTTAAATTCACGCAAATCAAATAGTGTTAAAAAACAAGGCTATGATATTAAAGCTAAAATTAAAGATTACTTTAATAACAATAAAGAGCGCTTATTAGCACTATATATTGCTACACATCAAAACGATAGTGATTTTTTGTTTAAATCAAACTTAAAAAATGATTCAAAAGTTTTTGCATTTGATCAAAGAACAATTAATATTATTAAAGAAAACAATGAAATTCATTTATTAAATTACTATGATAATTTAATTGATAAGTTAAATGATAAATTAATTAAATTTAATAAAAAAACATTTATTATTCCTAGTGTTGATAGTTTATATGATAATGGAATTAATACACCAATTTACTTAATTAAAGACCAAAATCAACAAATTAGTTTATTTCAAACTTTAAAAATAAAACTAGTGGGTAATAAAAATCTTATAGATGAAAATCAAAAATTATCTACAATGATTAATGATTATGTTGAAAACTTTGATATAAAAAAACACCTTGTTGCTAATAATAAAAACTCCCAATCAATTGTTTTTGACGATTATGTTATAAATAAAGTATTTAGTAAAGGAATAAACAGTGTTTTTGCAAATATTATTAAACAAGAACAAATTAAAAAAAGTGTAGCAAAATTCTTTGAATTTAATGATTTTAATTTTAAAAATAATTTTCTTGAAAACCAAGAATTATTAGCAGCATTTAACCAAATTATTAAGAATACAAGTGTTTTGGAAACAACACAAACTAATAATTTTAGTAATTTTGATAACTTAAAAAAAGTTTATGAACAAGCTTTGAAATTATGA", "aa": "MNALLTELKNDFLYNGALYLNSRKSNSVKKQGYDIKAKIKDYFNNNKERLLALYIATHQNDSDFLFKSNLKNDSKVFAFDQRTINIIKENNEIHLLNYYDNLIDKLNDKLIKFNKKTFIIPSVDSLYDNGINTPIYLIKDQNQQISLFQTLKIKLVGNKNLIDENQKLSTMINDYVENFDIKKHLVANNKNSQSIVFDDYVINKVFSKGINSVFANIIKQEQIKKSVAKFFEFNDFNFKNNFLENQELLAAFNQIIKNTSVLETTQTNNFSNFDNLKKVYEQALKL", "aa_hexdigest": "c28ef9e8eddc836fddb72ad1646a27bb", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33426.675400000015, "isoelectric_point": 9.155300331115722 }, "id": "GPKLONFDEH_918", "locus": "GCA000006625_00897" }, { "type": "cds", "sequence": "contig_1", "start": 518699, "stop": 519349, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATAAATTAGTTTACGCTAATAACCCAAGTAAACAATTTAAAGGTGCACTATATGGTTATGGTAGTAGAGAATTATTAATTAAAACATTAGATGGTTTTGTGTCTTATCATGACTACCAAACACTTGCACAATTACTTTATGATCTAAATTTTAATGCAGAAGCTAATGCTGAATTTAAAACTATTTTAGATAACCAAAAACAAATTATTCCTGGCAATCAAAAAGATAGTAAAATAAAAAATATTGATTTAGCAACTAATGAAATTAAAACAATTATGATTAAAGGAATTAATAAAATCCCCCAAACTGCCTTTCAAAGATTAGATTTAGATTTAGTTTATAACAAATTAGACAAAAATGCTACACCATTAGTTAATAAAGAAGATAATGCTAATTTAAAATATATTTTATTAGTTTCACAATTTAATGCAAATGATGTGCAAAATTTAGTTATTAACAATCAATTAAATCAAAGTGCAACAGGATATTTAGGATTGTCAAAAAGCCAATTTTATAACTTATTATTTGATTTAGCATTAAATGATCAAACTTTAAAAATTGAAGCTTTTAGTCAAATTGAAAACGAACATAAAATTAAAGTTTACGATAAACGTTTAGCTAATAATAGTAATTTAAAAGGTTGA", "aa": "MFDKLVYANNPSKQFKGALYGYGSRELLIKTLDGFVSYHDYQTLAQLLYDLNFNAEANAEFKTILDNQKQIIPGNQKDSKIKNIDLATNEIKTIMIKGINKIPQTAFQRLDLDLVYNKLDKNATPLVNKEDNANLKYILLVSQFNANDVQNLVINNQLNQSATGYLGLSKSQFYNLLFDLALNDQTLKIEAFSQIENEHKIKVYDKRLANNSNLKG", "aa_hexdigest": "710ce48c3f9f75190c4449b596ea19b5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24618.704700000013, "isoelectric_point": 8.510745429992678 }, "id": "GPKLONFDEH_919", "locus": "GCA000006625_00898" }, { "type": "cds", "sequence": "contig_1", "start": 519367, "stop": 520026, "strand": "+", "frame": 1, "gene": "rpe", "product": "D-ribulose-5-phosphate 3 epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "RefSeq:WP_006688574.1", "SO:0001217", "UniParc:UPI00000C1C75", "UniRef:UniRef100_Q9PQ34", "UniRef:UniRef50_A0AAP9D7K6", "UniRef:UniRef90_Q9PQ34" ], "nt": "ATGAATAAGCAACTAGTTCCATCCCTTTTAGCATTTGATAAAAAATATCCTTATGAACAATTAAAGATCTTTAAAAAGCATCATCTTCAAACAATACATTATGATGTTATGGATCTAACATATGTTAATAACACTGCTTTTGAAACTGAACATTTAGAATGGCTACTAAAAAATGATTTTTTAGTACACATTCATCTAATGGTTATTAACCCTTTAGAAAAAATTAAACAATATTTTATATATAAACCAACTCGCATATCTTTTCATTTTGAAATGAATGATTATCAAACTAATTTATTAATCATTAATGAAATTAAAAAACATCATGTTTTAGCAGGGATTGCAATTCATCCTTGTTTATCACTAAACGATTATGCTTCTTATTTAAAAGAAGTTGATTATATTACTTTTATGTCGGTTATTCCAGGTAAGGGTGGACAAATTTTTTTAGAAGAAAGTTTATTAAAACTAAAAAAATTATATGAATATAAAATTAAAAATAATTTCAAATTTAAGATTGAAATTGATGGTGGTATTACTTTAGAAGTTATTAAAAAAATCAATATTCCAATTGATTTTTTTGTAAGCGGTTCTTATTTAGTGAAAAATATTGAAAAACTAGAGCAAACTGTTAACGAATTTAATCAACTTGTTAATTAA", "aa": "MNKQLVPSLLAFDKKYPYEQLKIFKKHHLQTIHYDVMDLTYVNNTAFETEHLEWLLKNDFLVHIHLMVINPLEKIKQYFIYKPTRISFHFEMNDYQTNLLIINEIKKHHVLAGIAIHPCLSLNDYASYLKEVDYITFMSVIPGKGGQIFLEESLLKLKKLYEYKIKNNFKFKIEIDGGITLEVIKKINIPIDFFVSGSYLVKNIEKLEQTVNEFNQLVN", "aa_hexdigest": "c5ed277c2cd4fa9a73e2cc8924282608", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C75", "ncbi_nrp_id": "WP_006688574.1", "uniref100_id": "UniRef100_Q9PQ34", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C75", "RefSeq:WP_006688574.1", "UniRef:UniRef100_Q9PQ34" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ34", "uniref90_id": "UniRef90_Q9PQ34" }, "psc": { "uniref90_id": "UniRef90_Q9PQ34", "gene": "Rpe", "product": "D-ribulose-5-phosphate 3 epimerase", "uniref50_id": "UniRef50_A0AAP9D7K6", "cog_id": "COG0036", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D7K6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D7K6" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [ "rpe" ], "id": "GPKLONFDEH_920", "locus": "GCA000006625_00899" }, { "type": "cds", "sequence": "contig_1", "start": 520153, "stop": 521841, "strand": "-", "frame": 1, "gene": "pheT", "product": "phenylalanine--tRNA ligase subunit beta", "db_xrefs": [ "COG:COG0073", "COG:J", "EC:6.1.1.20", "GO:0000049", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0006432", "GO:0009328", "KEGG:K01890", "SO:0001217", "UniRef:UniRef50_Q9PQ33", "UniRef:UniRef90_Q9PQ33" ], "nt": "GTGCGGATTTTATCTGATGATGTTAATTCGTATGGAATAATCGAGGTTAAAAATATTCAAAATTATATTTTAAAATGGAATACTAAAAGTATATTGATTAATAATCAAATTAAAGTTCTTAATAATTTCGCTGATAATATGAATTTTTTAACATTATTAACTGCTAATCCACTTCATGCATTTGATGCACGAAAAATTAGTGGTCAAATTGTTGTTAAAAATGCTGAAGAAGATGCAATTTTATTGGGTTTAGATCAAAAAGAATATTTAATTAAAAAAGGGGATCTAATTATCGTTGATGATCAAAAAATTTTAGCACTTGCTGGAATTATTGGTTCTAATGATTCTAAAATTGATGCAACAAGCACAACTGCTTATATTGAATGTGCAAATTTTAATCCTATGTTGATTGCAAATACAGCGCGTCGTTTAAAAATCAATACCGCAGCGGCTATGCGTTTTAGCAAACCCTTAACTAATTATGTTACAAAAGTAACATTAAAAAAATTGTTAGCTAATTTTAAATCAGATGCAAAATTAATTTATTATTTTAAACATTCAGTTCATAATGTCATTAAAAATAAAATTAATCAAGTAAGTGATTTTGTGGGTGCAGAAATTGATTTAGACACTGCTCAAACATTTTTAAAACGCTTAGGTTATAAAATTAATAAGTCTAATTTAATTACTCCAAGTCATCGATATGATGTATTAAATGAGTTTGATGTTTATGAAGATATTATGAAAAAAATTAGTATTCAAGAAATCAAACCACAACCAATCAGTTTTGATATTTTAAATTTTGAAAATAATCTAGCTTATGATTTTGAAAAAAAAGTTAGTGATTTTTTAGTTGATCAAGGTTTGTTTGAGTGTAAAACTTATAATTTAAAAAATCAAACACAAGCACATGAGTTTAATTTTTTTAATTTTAAACAAGCTTATGAAATTAATAATCCAACTTCAAATATGCGTTCGCATTTAAAATTAAATAACCTTAACTCCTTACTTGAAGTATTAGAATATAACCAAAACCAAAAAAATGAATTAGAAAATATCTTTGAGATTTCAAAGATTAATCCAGTTGATTCAAGTCAACAAACAGTTTTATCAATCATTTTATGTAAACCATTAATTAATTCAAAAATTAATGATTCATTAATAGTTAATAATTTTGTGACAACAAAAGCTTTACTACATGCTTTATTAACAAAATTAAATATTGATTATGCATATGATAAAAATCATGTTGTTAATGAATTATATGATAATAACCAATTAGCTTTAATTAATGCAAACAAACAAGTATTTGGTTTTATTGGTCAACTAAAAAATCAAGTCAAAAAAACATATGGTTTAAGTAATGATATTTTTATTATTAATTTAAATTTAACTAGTTATTTAAATCAAAAACAAGTAATCACTAAAGTTATTAAGCCAAGTATGTATCATGATGTCATACGTGATATTAGTGTTAAATTAGCTTCTGACGTTGACTTAAATAATATAATTGCTAATATTAAAAAAATTAAAAATATTAGAAAAGTTGAAATCAGCGATCTTTATATAAAAGATGATGGAATTATTTATACTTTTAAATATTATATTAATGATCATTTATCTAATTTAAGTAGTGAACAAATTATAATCATTGAACAAGAAGTAAATAATTATTTGAAACAATTTTAA", "aa": "MRILSDDVNSYGIIEVKNIQNYILKWNTKSILINNQIKVLNNFADNMNFLTLLTANPLHAFDARKISGQIVVKNAEEDAILLGLDQKEYLIKKGDLIIVDDQKILALAGIIGSNDSKIDATSTTAYIECANFNPMLIANTARRLKINTAAAMRFSKPLTNYVTKVTLKKLLANFKSDAKLIYYFKHSVHNVIKNKINQVSDFVGAEIDLDTAQTFLKRLGYKINKSNLITPSHRYDVLNEFDVYEDIMKKISIQEIKPQPISFDILNFENNLAYDFEKKVSDFLVDQGLFECKTYNLKNQTQAHEFNFFNFKQAYEINNPTSNMRSHLKLNNLNSLLEVLEYNQNQKNELENIFEISKINPVDSSQQTVLSIILCKPLINSKINDSLIVNNFVTTKALLHALLTKLNIDYAYDKNHVVNELYDNNQLALINANKQVFGFIGQLKNQVKKTYGLSNDIFIINLNLTSYLNQKQVITKVIKPSMYHDVIRDISVKLASDVDLNNIIANIKKIKNIRKVEISDLYIKDDGIIYTFKYYINDHLSNLSSEQIIIIEQEVNNYLKQF", "aa_hexdigest": "e7b687a87f4ad6831427b1d35ce4713d", "start_type": "GTG", "rbs_motif": null, "genes": [ "pheT" ], "truncated": "3-prime", "pseudogene": { "start": 520153, "stop": 522141, "observations": { "mutation": [ 521931 ], "stop": [ 521931 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ33", "query_cov": 1.0, "subject_cov": 0.727979274611399, "identity": 0.998, "score": 1078.0, "evalue": 0.0, "gene_start": 1, "gene_end": 562, "reference_start": 211, "reference_end": 772, "reference_sequence": "MILSLNLLHKISPKLKKIPLNELCAALMDLGCEVETINSIKPSTNLVFAKVLEKTRHPNANHLNLVKVKANQKVYEIVCGANNFSVHNWVVLAKLNAELANGLKITPRELRGYVSNGMLCAYSEINPQATSFLTSTDLDGILVLDDNYDHYKTPNQIFNLDDVILDLSIPSNRNDLNGYFWIAKELCAYFDFEYVVDATINHRPHKEIIDVRILSDDVNSYGIIEVKNIQNYILKWNTKSILINNQIKVLNNFADNMNFLTLLTANPLHAFDARKISGQIVVKNAEEDAILLGLDQKEYLIKKGDLIIVDDQKILALAGIIGSNDSKIDATSTTAYIECANFNPMLIANTARRLKINTAAAMRFSKPLTNYVTKVTLKKLLANFKSDAKLIYYFKHSVHNVIKNKINQVSDFVGAEIDLDTAQTFLKRLGYKINKSNLITPSHRYDVLNEFDVYEDIMKKISIQEIKPQPISFDILNFENNLAYDFEKKVSDFLVDQGLFECKTYNLKNQTQAHEFNFFNFKQAYEINNPTSNMRSHLKLNNLNSLLEVLEYNQNQKNELENIFEISKINPVDSSQQTVLSIILCKPLINSKINDSLIVNNFVTTKALLHALLTKLNIDYAYDKNHVVNELYDNNQLALINANKQVFGFIGQLKNQVKKTYGLSNDIFIINLNLTSYLNQKQVITKVIKPSMYHDVIRDISVKLASDVDLNNIIANIKKIKNIRKVEISDLYIKDDGIIYTFKYYINDHLSNLSSEQIIIIEQEVNNYLKQF" }, "paralog": false, "identity": 0.9984894259818731, "score": 1302.0, "evalue": 0.0, "description": "Internal stop codon at 521931. Nonsense mutation around 521931.", "psc": { "uniref90_id": "UniRef90_Q9PQ33", "gene": "pheT", "product": "phenylalanine--tRNA ligase subunit beta", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_Q9PQ33", "cog_id": "COG0073", "cog_category": "J", "kegg_orthology_id": "K01890", "go_ids": [ "GO:0000049", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0006432", "GO:0009328" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ33", "EC:6.1.1.20", "UniRef:UniRef50_Q9PQ33", "COG:COG0073", "COG:J", "KEGG:K01890", "GO:0000049", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0006432", "GO:0009328" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ33" ], "product": "Phenylalanine--tRNA ligase beta subunit" } }, "id": "GPKLONFDEH_921", "locus": "GCA000006625_00900" }, { "type": "cds", "sequence": "contig_1", "start": 521929, "stop": 522120, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATGTGCTTACAGTGAAATTAATCCACAAGCTACTAGTTTTTTAACATCAACAGATTTAGATGGAATTTTAGTTTTAGATGACAATTATGACCATTATAAAACCCCAAATCAAATTTTTAATTTAGATGATGTTATTTTAGATTTATCAATTCCATCAAATCGGAATGATTTAAATGGTTATTTTTGA", "aa": "MLCAYSEINPQATSFLTSTDLDGILVLDDNYDHYKTPNQIFNLDDVILDLSIPSNRNDLNGYF", "aa_hexdigest": "657086520ff1e5595b1c739210a070e8", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7157.817400000002, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_922", "locus": "GCA000006625_00901" }, { "type": "cds", "sequence": "contig_1", "start": 522205, "stop": 522471, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGATTTTAAGTTTAAATTTATTACATAAGATTAGTCCAAAACTAAAAAAGATTCCTTTAAATGAATTATGCGCAGCTTTAATGGATTTAGGTTGTGAAGTTGAAACAATTAATAGCATTAAACCATCTACTAATTTAGTTTTTGCTAAAGTATTAGAAAAAACTAGACATCCTAATGCTAATCATTTAAATTTAGTTAAAGTTAAAGCAAACCAAAAAGTTTATGAGATCGTTTGTGGTGCAAATAATTTTAGTGTTCATAATTGA", "aa": "MILSLNLLHKISPKLKKIPLNELCAALMDLGCEVETINSIKPSTNLVFAKVLEKTRHPNANHLNLVKVKANQKVYEIVCGANNFSVHN", "aa_hexdigest": "307604df82f8df316d19e73bbf5beeb8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 41, "aa_cov": 0.45454545454545453, "hmm_cov": 1.0, "evalue": 3.638981428686506e-08, "score": 29.035503387451172, "start": 46, "stop": 85 } ], "seq_stats": { "molecular_weight": 9788.509299999994, "isoelectric_point": 9.334393501281738 }, "id": "GPKLONFDEH_923", "locus": "GCA000006625_00902" }, { "type": "cds", "sequence": "contig_1", "start": 522503, "stop": 522664, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGCTACATGAAAATGTTCTAAAAGCTGCGAATATTAACGATATTAGAACAGGGATGGCTTTTGGTGTGGGTATTGATCGAATTGCAATGTTAAAATATGAAATTGATGATATTCGTTATTTATATTCAAATAATTTTAAATTTAATGCCCAAATTAAATAA", "aa": "MLHENVLKAANINDIRTGMAFGVGIDRIAMLKYEIDDIRYLYSNNFKFNAQIK", "aa_hexdigest": "abb01eab37d281a2739143d251a43dce", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 51, "aa_cov": 0.9622641509433962, "hmm_cov": 1.0, "evalue": 3.804401430454957e-19, "score": 64.69293975830078, "start": 1, "stop": 51 } ], "seq_stats": { "molecular_weight": 6150.072499999999, "isoelectric_point": 8.085383033752443 }, "id": "GPKLONFDEH_924", "locus": "GCA000006625_00903" }, { "type": "cds", "sequence": "contig_1", "start": 522878, "stop": 523237, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "TTGTTTGCTCCTTTTATTAATAATGGGCATCATCATATTCTCAATAGTATTATTGATGATATTGCATCTTTTTTTAAAAAATTAAATTTTGAAATTGTAAGTGGTTCAGAAGTTGTTTCACCTATTTATAATTTTGATCATTTAAATATTGATGAAAATCATCCAGCAAGAGCAAGTGCTGATTCTTTTTTTATTAATTCAATTAAAATGTTACGTACGCATTGCACAACTACAACCGCACAATTTTTAGAAAATAACGTAAACAAGGACATTAGAATTATGTCTTTTGGTAATGTTTATCGAAAAGATGATGATGATGCAACACATAGCCACCAATTCAATCAAGTTGATTTTGTATGA", "aa": "MFAPFINNGHHHILNSIIDDIASFFKKLNFEIVSGSEVVSPIYNFDHLNIDENHPARASADSFFINSIKMLRTHCTTTTAQFLENNVNKDIRIMSFGNVYRKDDDDATHSHQFNQVDFV", "aa_hexdigest": "8f7e10288f831c7756f96bb3c8527195", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 123, "aa_cov": 0.9747899159663865, "hmm_cov": 1.0, "evalue": 1.4720942482010862e-38, "score": 128.2692413330078, "start": 4, "stop": 119 } ], "seq_stats": { "molecular_weight": 13675.073099999983, "isoelectric_point": 5.635040473937988 }, "id": "GPKLONFDEH_925", "locus": "GCA000006625_00904" }, { "type": "cds", "sequence": "contig_1", "start": 523250, "stop": 523504, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTATTAATCTAATTAAAAATTTAAAACAAATTTTACGAACAGCAAATAATGAACGTCATTTGATTGAATTAAAAAATATTTTTGTTAAACAACATTTATTACCACTATATGATGAATTAAAAAAAAGCGCTAATAAAAAAGAAATGGGTTTATTAATTAATAATTTCAAACAACAAATTGAATTTGTTACTGATCAAATTTTAAAAGAATTAAATGATAAAGATGATCAAGTTGATTTAAAAAAATGA", "aa": "MDVINLIKNLKQILRTANNERHLIELKNIFVKQHLLPLYDELKKSANKKEMGLLINNFKQQIEFVTDQILKELNDKDDQVDLKK", "aa_hexdigest": "ff00212cf1918553240dc94b37b96c90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9985.584799999993, "isoelectric_point": 8.892462730407715 }, "id": "GPKLONFDEH_926", "locus": "GCA000006625_00905" }, { "type": "cds", "sequence": "contig_1", "start": 523865, "stop": 523981, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAACAAAAAAGGTAAAAAATTTTATGATTTTTTAAAAACAATCGAGGGTTTTAAAATTATTACGATTATTTCATATGTTTTTGTAGCAATTTTATTAGCAATTTTAATCTGA", "aa": "MNNKKGKKFYDFLKTIEGFKIITIISYVFVAILLAILI", "aa_hexdigest": "c8e9157b3cd742ff30ea25d516f2980a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4407.3899999999985, "isoelectric_point": 9.778516197204588 }, "id": "GPKLONFDEH_927", "locus": "GCA000006625_00906" }, { "type": "cds", "sequence": "contig_1", "start": 523974, "stop": 524741, "strand": "-", "frame": 2, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "COG:COG0171", "COG:H", "RefSeq:WP_006689045.1", "SO:0001217", "UniParc:UPI00000C1C77", "UniRef:UniRef100_Q9PQ30", "UniRef:UniRef50_Q9PQ30", "UniRef:UniRef90_Q9PQ30" ], "nt": "ATGAATAATGATTTGATTAAATATCTACAATGGTTGAAAAATGATTTTATAAAATTTTTAAATGACGCACAAACGAATAATGTTATTTTGGGCATTTCAGGTGGTATTGATAGCACTTTAACATTAGCTATTTTAAATGATTTAAAAAAAGAGTATGATCTTAATATTTATGCTTATTTTTTAGATATTCATAATTCGAGTTTAGATTATGAATGTATTAATGAATTAAAAAATATTTATCCTAACATTGAAGTTATCAATCTTGTTGATATCTATGATAGCTATTGTAAAATGATTAATAAAAAAACAAATGATAAATATGTTCTTTATAATTTAAAGCCTAAAATTCGTACAAATTATTTATATGCAATGGCGAATGCATACAAGGGTGTTGTTGTGAGTAATTTAAACTATGATGAGTACATTTTAGGTTTTTTTACTAAATATGGCGATAGTGCCGCTGATTATTATATGTTAATTGGTTTACTAAAAAAACATATTTATGAATTAGGTGCTTATTATCATTTACCAAATAAAATTTTAAACCGTGCTCCTACTCCAGCAAATGAAGATGATGAACACAAAACTGATGAGAGTTTTTTTGGTTTTACATATAACGATCTTGATCAATTTTTACTTTATCGTAAAATTAATCCTAAAATTGTTAGCATGATTAAAAAACGTTATGAGACTAATGCTCATAAACATTTTGTTTTTGATAAAAAGAAATTATTTTTAAATTATAAATTAGGAAATAAACATGAATAA", "aa": "MNNDLIKYLQWLKNDFIKFLNDAQTNNVILGISGGIDSTLTLAILNDLKKEYDLNIYAYFLDIHNSSLDYECINELKNIYPNIEVINLVDIYDSYCKMINKKTNDKYVLYNLKPKIRTNYLYAMANAYKGVVVSNLNYDEYILGFFTKYGDSAADYYMLIGLLKKHIYELGAYYHLPNKILNRAPTPANEDDEHKTDESFFGFTYNDLDQFLLYRKINPKIVSMIKKRYETNAHKHFVFDKKKLFLNYKLGNKHE", "aa_hexdigest": "8a5ed024897ce9cc5ce2bb20a55110fa", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C77", "ncbi_nrp_id": "WP_006689045.1", "uniref100_id": "UniRef100_Q9PQ30", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C77", "RefSeq:WP_006689045.1", "UniRef:UniRef100_Q9PQ30" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ30", "uniref90_id": "UniRef90_Q9PQ30" }, "psc": { "uniref90_id": "UniRef90_Q9PQ30", "gene": "NadE", "product": "NH(3)-dependent NAD(+) synthetase", "uniref50_id": "UniRef50_Q9PQ30", "cog_id": "COG0171", "cog_category": "H" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ30" ], "product": "NH(3)-dependent NAD(+) synthetase" }, "genes": [ "nadE" ], "id": "GPKLONFDEH_928", "locus": "GCA000006625_00907" }, { "type": "cds", "sequence": "contig_1", "start": 525004, "stop": 525789, "strand": "-", "frame": 1, "gene": "obgE", "product": "GTPase ObgE", "db_xrefs": [ "COG:COG0536", "COG:DL", "EC:3.6.5.-", "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_Q9PQ29", "UniRef:UniRef90_Q9PQ29" ], "nt": "GTGCCACTAGGAACAGTTGTTTATAATCCTATCACTAATGAAATTTTGGCAGATATTAATATTGATCAACAAAAATATTTAGTTGCCCAAGGTGGTTTAGGCGGTCATGGAAATACACATTTTAAATCGCCTTTTAATAAAGCACCAAATTTATATGAATTAGGAGAATTAGGTGAAAATGTTGAAGTTTTATTAGAATTAAAAACTATTGCTGATATTGGAATTATAGGATTACCAAATGCAGGAAAATCAACATTAATTTCTACATTTACAAATGCTAAACCAAAAACGGCAAATTACATGTTTACAACTTTAAATCCCGTTTTAGGTACAATTTATCGTGATCAAAATCGTATCATTTTTGCAGATATTCCTGGTTTAATTGAAGGAGCACATACAGGAGTTGGCTTGGGGCATGATTTTTTAAAACATATCGAACGTTGTTTTTTATTAATTCATTTAATTTCTTTAGATCCTAATGATAATCCTGATATTATTAATGCTTATGAAACGATTGTTAATGAATTAAAACAATATAAACAAAATTTAGTTAACAAACCAATTGTATTGGTTGCTAATAAAATAGATCAAATTGGAGCATTAGAAAATTTGCAGATTCTAAAAGAATATTTAAAAAATAATCAAGAAATTAAAATTATATCTGCGTTAACAAATTTACATGTAGATAATATGTTAGATGATGTTATTAAAATTTACTTTGCACAAAAAAAGATTTATGAGCAACGACTTAAAGAACAATTACCAGTTGATCAAATTTTAAAATGA", "aa": "MPLGTVVYNPITNEILADINIDQQKYLVAQGGLGGHGNTHFKSPFNKAPNLYELGELGENVEVLLELKTIADIGIIGLPNAGKSTLISTFTNAKPKTANYMFTTLNPVLGTIYRDQNRIIFADIPGLIEGAHTGVGLGHDFLKHIERCFLLIHLISLDPNDNPDIINAYETIVNELKQYKQNLVNKPIVLVANKIDQIGALENLQILKEYLKNNQEIKIISALTNLHVDNMLDDVIKIYFAQKKIYEQRLKEQLPVDQILK", "aa_hexdigest": "035d742d431377a115ecb2e19057981b", "start_type": "GTG", "rbs_motif": null, "genes": [ "obgE" ], "truncated": "both", "pseudogene": { "start": 524749, "stop": 526056, "observations": { "mutation": [ 524904, 525006, 525921, 525990 ], "stop": [ 524904, 525006, 525921, 525990 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ29", "query_cov": 1.0, "subject_cov": 0.6, "identity": 0.996, "score": 516.0, "evalue": 1.86e-182, "gene_start": 1, "gene_end": 261, "reference_start": 90, "reference_end": 350, "reference_sequence": "MAFIDKCKIVLIAGNGGDGIVSWRRETHVPEGGPAGGNGGNGGSIWFVGNHNETSLEFLKYKKIIRAKHGEKGDIKNQHGANAEDVFINVPLGTVVYNPITNEILADINIDQQKYLVAQGGLGGHGNTHFKSPFNKAPNLYELGELGENVEVLLELKTIADIGIIGLPNAGKSTLISTFTNAKPKTANYMFTTLNPVLGTIYRDQNRIIFADIPGLIEGAHTGVGLGHDFLKHIERCFLLIHLISLDPNDNPDIINAYETIVNELKQYKQNLVNKPIVLVANKIDQIGALENLQILKEYLKNNQEIKIISALTNLHVDNMLDDVIKIYFAQKKIYEQRLKEQLPVDQILKWTSDIPKSKELDKTIEIIKVDDHIFEVFGEYLKYWAHRIPLKTQDNLIRFNQKLQSINFNQQLLQAGAVAGDSIKIYDITLEFEE" }, "paralog": false, "identity": 0.9885057471264368, "score": 851.0, "evalue": 4.7e-317, "description": "Internal stop codon at 524904, 525006, 525921, 525990. Nonsense mutation around 524904, 525006, 525921, 525990.", "psc": { "uniref90_id": "UniRef90_Q9PQ29", "gene": "obgE", "product": "GTPase ObgE", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_Q9PQ29", "cog_id": "COG0536", "cog_category": "DL", "go_ids": [ "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ29", "EC:3.6.5.-", "UniRef:UniRef50_Q9PQ29", "COG:COG0536", "COG:DL", "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ29" ], "product": "GTPase Obg" } }, "id": "GPKLONFDEH_929", "locus": "GCA000006625_00908" }, { "type": "cds", "sequence": "contig_1", "start": 526059, "stop": 526253, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAATTACAAATGCTATTATGAATCGCTCAAAACATTTTGAGACATATGCTGCAGATCTATACAATGAACTTAAAGATTCAGAACAATATTATAATGAATTTAACAAAAAACGTTTAGAATTAAAAAAGCGTAATAAAGCAAATGAACGAATAAAATATAAAGTTGAAGATGATCAAAAAGGAAAATAA", "aa": "MTKITNAIMNRSKHFETYAADLYNELKDSEQYYNEFNKKRLELKKRNKANERIKYKVEDDQKGK", "aa_hexdigest": "986c80c2a55aa74d9c49e1a65e80c1e8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7819.758199999999, "isoelectric_point": 9.486925697326662 }, "id": "GPKLONFDEH_930", "locus": "GCA000006625_00909" }, { "type": "cds", "sequence": "contig_1", "start": 526387, "stop": 526518, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCAACGAATCTTTGGCGTACTAAAAGCAAATATAATACATACAATAACTGCTAATCCTAAAGTTGAAAACATTGCAACTCAAACTGGATATGAACTAATTATATTTGTACCAAATTCAACACCATGA", "aa": "MSQRIFGVLKANIIHTITANPKVENIATQTGYELIIFVPNSTP", "aa_hexdigest": "bc278ae3836ad1f995dd8639a3d61a42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4741.4659, "isoelectric_point": 8.27550029754639 }, "id": "GPKLONFDEH_931", "locus": "GCA000006625_00910" }, { "type": "cds", "sequence": "contig_1", "start": 526470, "stop": 526568, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAAGAATGCAAAAATTAGTTAATGAAATTACTAATCAATACAATCATGGTGTTGAATTTGGTACAAATATAATTAGTTCATATCCAGTTTGA", "aa": "MIERMQKLVNEITNQYNHGVEFGTNIISSYPV", "aa_hexdigest": "d6df553d71264a00bd05a82b4de9f3a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3726.1968000000015, "isoelectric_point": 5.478108024597168 }, "id": "GPKLONFDEH_932", "locus": "GCA000006625_00911" }, { "type": "cds", "sequence": "contig_1", "start": 526944, "stop": 527861, "strand": "-", "frame": 2, "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "db_xrefs": [ "COG:COG0223", "COG:J", "EC:2.1.2.9", "GO:0004479", "GO:0005737", "GO:0005829", "GO:0071951", "RefSeq:WP_010891782.1", "SO:0001217", "UniParc:UPI000012AB14", "UniRef:UniRef100_Q9PQ27", "UniRef:UniRef50_Q9PQ27", "UniRef:UniRef90_Q9PQ27" ], "nt": "ATGAAATATAAAGTAATGTTTTTTGGTACACCAGAAATCGCTAAAATTGTTTTGGAAACATTGTTTAACATGCATGAAGTTGATTTAATCGCTGTTGTTAGTCAACCAGATGCACATTTTGATCGAAAAAAGAATGTTATCTACTCTCCTGTTAAACAGTTTTGTTTAGATCATAATATTAAGTTATTTCAACCAGAAAAAATCAAAGAAATTGAAGAAGAAATCCGTATTTTAGGACCAGATATTATCATTACTTGTGCTTTTGGTCAATTTATTAATCAAGGGATAATTGATATTCCTAAATACAAAATCGTTAATATTCATGCTTCACTTTTACCAAAATTACGTGGCGGAGCACCAATTCATTATGCCATTTTAAATGGTGAATTAAAAACAGGAATCACTTTAATGCATACGATTAAAAAAATGGATGCAGGTAATATTTTATTTCAAAGAAGTTTAGAAATAAACGATTGCACAACAACCAAATCACTAACTTTAGAGTTAGCTAATCTTAGTGCATTGATGATTAAAGAACATTTTTTAGAATTAGTAAAACCAAATTTAGTTGGAATTCAACAAGATGAAAATTCTGTTAGTTTTGCTTATAATATTCAAAAGGACCAAAATATTATTAATTTTGACCAACCAGCTTTTTTTATTAATCGTTTTGTTAATGCTATGTATGATAAACCAATCGCTATTATGCAATATAATGGCGTTTCAATTAAAGTATATCAAGTTAAAATCACTAATCAAAAGTCATGTCAAAAACCTGGAACAATCATGATTTTTAAAAATCAACTTTTTGTAAGTACGCAAGATTTTGATATTGAACTATTATTGATTCAGCTTCCAAATAAAAAACCTTTATCTCCAAAAGTTTTATTGAATGGGAAAAACCCTTTTATTAATTAA", "aa": "MKYKVMFFGTPEIAKIVLETLFNMHEVDLIAVVSQPDAHFDRKKNVIYSPVKQFCLDHNIKLFQPEKIKEIEEEIRILGPDIIITCAFGQFINQGIIDIPKYKIVNIHASLLPKLRGGAPIHYAILNGELKTGITLMHTIKKMDAGNILFQRSLEINDCTTTKSLTLELANLSALMIKEHFLELVKPNLVGIQQDENSVSFAYNIQKDQNIINFDQPAFFINRFVNAMYDKPIAIMQYNGVSIKVYQVKITNQKSCQKPGTIMIFKNQLFVSTQDFDIELLLIQLPNKKPLSPKVLLNGKNPFIN", "aa_hexdigest": "7c6de2e3b3c47b72652517b106b963ce", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000012AB14", "ncbi_nrp_id": "WP_010891782.1", "uniref100_id": "UniRef100_Q9PQ27", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012AB14", "RefSeq:WP_010891782.1", "UniRef:UniRef100_Q9PQ27" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ27", "uniref90_id": "UniRef90_Q9PQ27" }, "psc": { "uniref90_id": "UniRef90_Q9PQ27", "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "ec_ids": [ "2.1.2.9" ], "uniref50_id": "UniRef50_Q9PQ27", "cog_id": "COG0223", "cog_category": "J", "go_ids": [ "GO:0004479", "GO:0005737", "GO:0005829", "GO:0071951" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ27" ], "product": "Methionyl-tRNA formyltransferase" }, "genes": [ "fmt" ], "id": "GPKLONFDEH_933", "locus": "GCA000006625_00912" }, { "type": "cds", "sequence": "contig_1", "start": 528064, "stop": 528357, "strand": "-", "frame": 1, "gene": null, "product": "DUF5385 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYK1", "UniRef:UniRef90_UPI00290991E4" ], "nt": "ATGACAAAACAACAAAGAATTGATTATAAAAATGAACAAAAAGCAATCAAAGCACTTAAAACAACGAATCCAGAGCAATATAAAATTGAAATGGAACGAATTAAAAAAGAAAAACGTGCTAAACCTAAAGAACTATATGTAGTTTTGTTTACTACAAGAAATGCTAAAACTTTAATTATAGATGAACCCCGTGCGATTGAGTGTGAAGTTCGTTTGGTAAAAATTAATAAAAAAGAAAACCGACGCGAAATTGATGTTGTGCGTGCTTTAGATTATGAAGAAGAAATGTTATGA", "aa": "MTKQQRIDYKNEQKAIKALKTTNPEQYKIEMERIKKEKRAKPKELYVVLFTTRNAKTLIIDEPRAIECEVRLVKINKKENRREIDVVRALDYEEEML", "aa_hexdigest": "364ccf2b1e689cf4a13b7c2e0b439041", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 527947, "stop": 528606, "observations": { "mutation": [ 528066, 528459 ], "stop": [ 528066, 528459 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00290991E4", "query_cov": 1.0, "subject_cov": 0.4092827004219409, "identity": 0.99, "score": 177.0, "evalue": 1.77e-54, "gene_start": 1, "gene_end": 97, "reference_start": 101, "reference_end": 197, "reference_sequence": "MGVRGWGLGECFSGVSDGGNGNFLMILIIIIPIVLVVVFIMRKKRKQNGENQTSVNNKDKSDTNEVWTTIKKYLRSIDDKGKEVIDSYVVKRAEPHNLAQMTKQQRIDYKNEQKAIKTLKTTNPEQYKIEMERIKKEKRAKPKELYVVLFTTRNAKTLIIDEPRAIECEVRLVKINKKENRREIDVVRALDYEEEMLWIEPIKAKDDEIYNKRLEADKKKQQKAAQRRQKQLEKQKK" }, "paralog": false, "identity": 0.9817351598173516, "score": 371.0, "evalue": 2.91e-133, "description": "Internal stop codon at 528066, 528459. Nonsense mutation around 528066, 528459.", "psc": { "uniref90_id": "UniRef90_UPI00290991E4", "product": "DUF5385 domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00290991E4", "UniRef:UniRef50_A0A2C9DYK1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYK1" ] } }, "id": "GPKLONFDEH_934", "locus": "GCA000006625_00913" }, { "type": "cds", "sequence": "contig_1", "start": 528457, "stop": 528660, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAACCAAGAAATAATAGAATCTATTTTACAAGCAGATAGTGCAAAGGGTGGTAATGGCAACTTTTTAATGATCTTAATCATTATCATTCCAATTGTATTAGTTGTAGTATTTATTATGCGTAAAAAACGTAAACAAAATGGTGAAAATCAAACTAGTGTTAATAACAAAGATAAGTCAGATACAAATGAAGTTTGA", "aa": "MLDNQEIIESILQADSAKGGNGNFLMILIIIIPIVLVVVFIMRKKRKQNGENQTSVNNKDKSDTNEV", "aa_hexdigest": "c76e4980f58b1afcb2bb49966e3cf44d", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7499.642500000004, "isoelectric_point": 6.125674629211424 }, "id": "GPKLONFDEH_935", "locus": "GCA000006625_00914" }, { "type": "cds", "sequence": "contig_1", "start": 529063, "stop": 529155, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCCGTTATATTGATGTTTGTTATCATAATCAAGCCAAAAAATATAAAATTCGTTCAGGAATTGCGATCGCTGCAAATCAAGTTGGTTGA", "aa": "MVRYIDVCYHNQAKKYKIRSGIAIAANQVG", "aa_hexdigest": "76299d926cea718333e551350547782c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3410.9673000000003, "isoelectric_point": 9.744928169250485 }, "id": "GPKLONFDEH_936", "locus": "GCA000006625_00915" }, { "type": "cds", "sequence": "contig_1", "start": 529513, "stop": 529632, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATAAATACGATGGGAATGCTATTAAAATCTTAGAAGGGTTGGAAGCTGTTCGTAAGCGTCCTGGAATGTATATTGGCTCTACAAGTAGCACTGGATTACATCATTTAATTTGA", "aa": "MANKYDGNAIKILEGLEAVRKRPGMYIGSTSSTGLHHLI", "aa_hexdigest": "cc63cf1ba3b673bd2d1eadb1783cf925", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4242.878099999999, "isoelectric_point": 9.397830390930174 }, "id": "GPKLONFDEH_937", "locus": "GCA000006625_00916" }, { "type": "cds", "sequence": "contig_1", "start": 529663, "stop": 529896, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGAATGCTAACGCAAAAAACATTAATGTTATTTTGCATGAAGATAATTCAATTAGTGTTTTAGATGATGGGCGTGGTATACCAGTTGACATTAATTCTCAAACAAAAATTTCTACTGTTGAAACTGTTCTAACAGTTTTGCATGCTGGAGGTAAGTTTGATGAAAGTGCATACAAAACTGCTGGTGGTTTACATGGTGTTGGTTCATCAGTAGTTAATGCTTTAAGTTCTTGA", "aa": "MNANAKNINVILHEDNSISVLDDGRGIPVDINSQTKISTVETVLTVLHAGGKFDESAYKTAGGLHGVGSSVVNALSS", "aa_hexdigest": "ef9bf87c2282da8228433799e10a423d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 76, "aa_cov": 0.948051948051948, "hmm_cov": 0.8157894736842105, "evalue": 2.1881708491477994e-09, "score": 33.37578201293945, "start": 4, "stop": 76 } ], "seq_stats": { "molecular_weight": 7948.7559, "isoelectric_point": 5.349140357971191 }, "id": "GPKLONFDEH_938", "locus": "GCA000006625_00917" }, { "type": "cds", "sequence": "contig_1", "start": 530302, "stop": 530592, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA87CUZ8", "UniRef:UniRef90_A0AA87CUZ8" ], "nt": "ATGGTTTCATTTGCCAATTCTGTGAAAACTAGTGAAGGTGGTGTACACGAAAATGCTTTTAAAAACGCTTTAACAAGTGTTGTTAATAATTATGCAAGAAAACATAATTTACTTAAAGAAAAAGACAAAAATTTAGAAGGTGATGATATACGTGAGGGTTTATCAAGTGTAATTTCACTTCGAATCCCCGAAAGCTTAATTAGCTATGAGGGTCAAACTAAAAACAAATTATTCACACCAGAAGCAAATGAAGCAGTAAAAAAAACAATTGAAGATAATTTTAGTTTTTGA", "aa": "MVSFANSVKTSEGGVHENAFKNALTSVVNNYARKHNLLKEKDKNLEGDDIREGLSSVISLRIPESLISYEGQTKNKLFTPEANEAVKKTIEDNFSF", "aa_hexdigest": "6263b6ef365a6541284034443019e94b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA87CUZ8", "query_cov": 0.9166666666666666, "subject_cov": 0.9072164948453608, "identity": 1.0, "score": 168.0, "evalue": 1.39e-52, "valid": true, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0AA87CUZ8" }, "pscc": { "uniref50_id": "UniRef50_A0AA87CUZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA87CUZ8" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "GPKLONFDEH_939", "locus": "GCA000006625_00918" }, { "type": "cds", "sequence": "contig_1", "start": 530659, "stop": 531375, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971IXM9", "UniRef:UniRef90_A0AA86Z7L8" ], "nt": "TTGGCTGCAAAGCGTGCTCGTGAAGAAACTAAAAAAGTTAAAAAAATTAAAGAAGAGCGAGGAATGGGCGGAAAATTAACGCCAGCACAAAGTAAAGATCCAGTTTTAAATGAATTATTTTTGGTTGAAGGTGATTCAGCTGGTGGATCAGCTAAACTAGGACGAAATAAAAAATACCAGGCTATTTTGCCCTTAAGAGGTAAGGTTCTTAATGTTTTAAAAGCTCGTTTAGTTGATGTTTTAAAAAATGAAGAAATAGCATCAATCTTTACTTGTTTAGGAACAGGAATTGGTGCAGAATTTGATTTAAAAAAATTAAAATATCATAAAATTATTATTATGACCGATGCAGATACCGATGGTAGTCACATCCAAGTTTTATTATTAACTTTATTTTATCGTTTTATGCGACCTTTAATTGAAAATGGTAATATTTACATAGCTTTACCTCCGCTTTATAAATTAACAAATAAAAATAACAAAAAATTCTTTTATGCTTGGGATGATGTTGAATTAGATCAACTAAAAAAAGAGCAAAAAAATTATGAAATCCAACGTTATAAAGGTCTTGGCGAAATGAACGCTGATCAATTATTTGAAACAACAATGGATCCAAACAATCGTTTATTATTAAAAATTAATATTAATGACATTTTACAAGCTGAACGGCAAATCAATACTTTAATGGGTAATGACGTAAGTATTCGAAAGCAGTGA", "aa": "MAAKRAREETKKVKKIKEERGMGGKLTPAQSKDPVLNELFLVEGDSAGGSAKLGRNKKYQAILPLRGKVLNVLKARLVDVLKNEEIASIFTCLGTGIGAEFDLKKLKYHKIIIMTDADTDGSHIQVLLLTLFYRFMRPLIENGNIYIALPPLYKLTNKNNKKFFYAWDDVELDQLKKEQKNYEIQRYKGLGEMNADQLFETTMDPNNRLLLKININDILQAERQINTLMGNDVSIRKQ", "aa_hexdigest": "b25ae854e526e4a358b084a678365729", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA86Z7L8", "query_cov": 0.9117647058823529, "subject_cov": 0.9079497907949791, "identity": 0.9590000000000001, "score": 412.0, "evalue": 8.78e-145, "valid": true, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A971IXM9" }, "pscc": { "uniref50_id": "UniRef50_A0A971IXM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971IXM9" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "GPKLONFDEH_940", "locus": "GCA000006625_00919" }, { "type": "cds", "sequence": "contig_1", "start": 531447, "stop": 531734, "strand": "+", "frame": 3, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "nt": "ATGAGTGTAAATCATCAAAAAATTATTAATACACCTTTAGACAACATTGTTGGTGAAAGTTATGCAAAATATGCAAAATACATTATTCAAGATCGTGCTTTACCTGATATTCGTGATGGTTTAAAACCTGTTCAACGACGAATTTTATACGCAATGAGTGAATTAGGAATCTTTTATGATAAACCTTATAAAAAATCTGCACGTACAGTAGGAGAAGTAATTGGTAAATATCATCCACATGGAGATTCATCAATTTATGAAGCAATGGTGAGAATGAGTCAAGATTGA", "aa": "MSVNHQKIINTPLDNIVGESYAKYAKYIIQDRALPDIRDGLKPVQRRILYAMSELGIFYDKPYKKSARTVGEVIGKYHPHGDSSIYEAMVRMSQD", "aa_hexdigest": "2227b1e5f1916d3e870c0c5e3af460de", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "psc": { "uniref90_id": "UniRef90_UPI0028540D82", "query_cov": 0.8736842105263158, "subject_cov": 0.83, "identity": 0.6990000000000001, "score": 130.0, "evalue": 8.99e-38, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_Q9XCU2" }, "pscc": { "uniref50_id": "UniRef50_Q9XCU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "GPKLONFDEH_941", "locus": "GCA000006625_00920" }, { "type": "cds", "sequence": "contig_1", "start": 531759, "stop": 532775, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGCATGGTAATAAAGGTTCAATTGATGGTGATAATGCTGCTGCAATGCGTTATACAGAAGCTCGATTATCAAAAATTGCTAGTGTAATGTTAGCCAACTTAAAAAAAGATGTTGTTAAATTTAGTCCAAACTTTGATGATAGTGAAAAAGAGCCATCAATTTTACCATCTCTTTTTCCTAATTTGTTAATCAATGGAGCAACAGGAATTGCTTCTGGTTATGCAACAAATATTCCTCCTCATAACCCTAATGAAGTTTTTGATGCTTTAATTTATCGTATTGATCATCCAGATTGTAGTGTAGAAAAACTAATTGAAATCTGTCCTGCGCCAGATTTTCCCACAGGTGGGGAAATCCACGATTTAAATGGATGTGCTAATGCTCACAAAACTGGTGAAGGTAAATTTATAATTCGTGCTTGCATTGATTTTAAAATCAATGATAATAAGATTAATCAAATTATTATTAGTTCAATCCCTTATGAAACAAACAAGGCTTTAATCATTAAAGAAATTGAAGATATTATTTATAATAAAGAAGTTGCTGGATTAATCGAAGTTCGTGATGAATCTGATGCTAAGGGAATTAGTATAATTATTGATACTAAAAAAGATATTAATTTAGAAAATGTTAAAAACTATTTATATAAAAAAACCAACTTGGAAATCAGCTATAATACAAAATTTATTGCAATTGTTCACCGAACTCCTACACTTGTAAATCTATCTACATATTTAGATGCTCAAATTAATCATAGTTTAGATGTAATTAATAAAGTCGATTTATATGATCTAAACAAAATTTTGTTACGTATTGAAATTGTTGAAGGCTTAATTAAATGCGTTGATTTAATTGATGAAATTATTAAAATTATTCGTGCTAGTGAATCGCGTCAAGATGCAAAAAATACTTTAATTCAAGTTTTTAATTTTACAAATAATCAAGCTGAAGCGATTATTATGATGCGTTTACATAATTTGACACGTACTGATATATTTGATTTAAAAAATGAATGA", "aa": "MHGNKGSIDGDNAAAMRYTEARLSKIASVMLANLKKDVVKFSPNFDDSEKEPSILPSLFPNLLINGATGIASGYATNIPPHNPNEVFDALIYRIDHPDCSVEKLIEICPAPDFPTGGEIHDLNGCANAHKTGEGKFIIRACIDFKINDNKINQIIISSIPYETNKALIIKEIEDIIYNKEVAGLIEVRDESDAKGISIIIDTKKDINLENVKNYLYKKTNLEISYNTKFIAIVHRTPTLVNLSTYLDAQINHSLDVINKVDLYDLNKILLRIEIVEGLIKCVDLIDEIIKIIRASESRQDAKNTLIQVFNFTNNQAEAIIMMRLHNLTRTDIFDLKNE", "aa_hexdigest": "5a9af5b6ec8534e5319700870c667b5d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 339, "aa_cov": 1.0, "hmm_cov": 0.9734513274336283, "evalue": 3.5951431536598203e-96, "score": 318.1892395019531, "start": 1, "stop": 338 } ], "seq_stats": { "molecular_weight": 37921.08890000003, "isoelectric_point": 5.383073234558106 }, "id": "GPKLONFDEH_942", "locus": "GCA000006625_00921" }, { "type": "cds", "sequence": "contig_1", "start": 532857, "stop": 533168, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAATTTAAAAAAACTTTTGGTTTTGAACGCAAAACAAAATTATTTGATGAATTAGTAAAAGTTGAAATAAACGAAGATCAAATGATCGAAAAACAAAATTTGAATTTAGTAATAAGTCGTGATGGATACATTAAAACTGTTTCTAAAAAGTCATTTGAGTCATCTAAATATGATGAATTAGGATTAAAAACTAATGATATTCTTTTTTATCATAATATCATTAATTCTCATGATAAAATCCTAATCATTACATCAAAAGCTAAATTAATTAATTTAGTTGCTCATAAAATCACTTCTATGCGGTGA", "aa": "MIEFKKTFGFERKTKLFDELVKVEINEDQMIEKQNLNLVISRDGYIKTVSKKSFESSKYDELGLKTNDILFYHNIINSHDKILIITSKAKLINLVAHKITSMR", "aa_hexdigest": "87b32cc6154cec7ffd92bec03b9fdad4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12079.969499999983, "isoelectric_point": 9.30112781524658 }, "id": "GPKLONFDEH_943", "locus": "GCA000006625_00922" }, { "type": "cds", "sequence": "contig_1", "start": 533199, "stop": 533999, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03989.18" ], "nt": "GTGAAATTTGATGCTAATGAAAAAGTTATAGCTGTTTATATATGGAACGAACAATTTAAAATTGATGAATATCAATTAGTTTTGGCTTCTAGATTAAATCTAATTAAAAGAATTGAATTAAGCGAGTTGGATATAAATAAAAATAGTAAACAAATTAGCATTATGAAATTAAATGATAATGATAATTTAATCAGTGCTAATTTAATCAAAAAAGGTCATAATCAATTTATTATTGCAATTTCTAAATTAGGATTAGCCCTGCTATTTTTAGTTCATGAAATTAATTGCTTAAATCGTTTAGCTAAAGGAATTAAAATTATGAAATTAAAACCAAATGATGAGGTTAGTGCTATTTTAATTACTCCTAATAATGGTTATAATGTTCAACTTTTTTTAGAACGGGGAAGTAAGTGTTTTAATATTAGTGAATTAAAATTATCAAAACGTGCGGCCACACCAACAAATTTATATCCAATAACAAAAAAAGTGCAAAATGTTTTAGCAGCTTTTTTAGTTGCTCATGAAAATGTTTTTTATCTTTTAGATCAACAACAAAAAATAAATCCATATTACTTATCAAACCCCAAACCAACAAAACTAGATACTAAAATTAGTATCTATGAAAACGATCAAATGATCACTGATGTAGTTAAAGATTGCTTTTTAAGTGATGAAGCAATTAGTGATTTTAAAAAAATTAATATGTACGCAAATGAATTTGATAATGAAATGCTAAAAATTAATAAAAATCACGATGATTCACAATTAGAATTAATTGATGAAGAGGAAGAAAAAAAATAA", "aa": "MKFDANEKVIAVYIWNEQFKIDEYQLVLASRLNLIKRIELSELDINKNSKQISIMKLNDNDNLISANLIKKGHNQFIIAISKLGLALLFLVHEINCLNRLAKGIKIMKLKPNDEVSAILITPNNGYNVQLFLERGSKCFNISELKLSKRAATPTNLYPITKKVQNVLAAFLVAHENVFYLLDQQQKINPYYLSNPKPTKLDTKISIYENDQMITDVVKDCFLSDEAISDFKKINMYANEFDNEMLKINKNHDDSQLELIDEEEEKK", "aa_hexdigest": "920169bda61df7169f11a59c74070487", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03989.18", "name": "DNA_gyraseA_C", "length": 46, "aa_cov": 0.17293233082706766, "hmm_cov": 1.0, "evalue": 2.571164595005028e-20, "score": 67.26143646240234, "start": 76, "stop": 121 } ], "seq_stats": { "molecular_weight": 30714.20480000002, "isoelectric_point": 6.460854148864747 }, "id": "GPKLONFDEH_944", "locus": "GCA000006625_00923" }, { "type": "cds", "sequence": "contig_1", "start": 533999, "stop": 535084, "strand": "+", "frame": 2, "gene": "yqeH", "product": "ribosome biogenesis GTPase YqeH", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "RefSeq:WP_006688742.1", "SO:0001217", "UniParc:UPI00000C1C7D", "UniRef:UniRef100_A0AAC9T0H0", "UniRef:UniRef50_B5ZBX1", "UniRef:UniRef90_A0AAC9T0H0" ], "nt": "ATGATAACAAATAAAAAATGTAAAGGCTGTGGAGCTTTTTTGAATGATCAAATCAATACAATTGGATATACCCCAAAAATAAATGAAACAAAAACCAATTTATGCCAACGTTGTTTTAAATTAATACACTATAATAAACTTGAAAAAGTAAGTACTAATTTAAATCAAAGTATTAAAGAGACAATTAACACTTTAAATTTTAGTAATTTACAGATTTTTGCTGTATTAGATATTTTAGATTTAGAACACACAATTATTGATGAACTAAAAGAATATCAAAATAAAATTATTTTTTTAGTTAATAAAATTGATTTATTACCTCAGCGCTACAATAGTGAATTAGTTAATGAAAATGTTGTTAAAACACTTATTAATCATGGATTTATTGATCCTAAAATTATCTATGTTTCCACACATTCTAATACCAGTTTAAAAAAAGTTATGGATTATATTAAAAATGCAACTAATAAAAAACAAAAATCCATTTTTTTTGGAAAATCAAATGTTGGTAAATCAAGTTTAATCAATGCTTTATTAGCGCTAAATAAAATTAAAACTAAACTTACAATTAGTAGTTATACTAATACAACAATTAATTTAAATAAAATTAATATACAAGAACATCAAATACTTGATGCTCCCGGAGTTTGTTTTGATGAAAACATTTTAAATTATGTACGTGATGAAGATAATAAATCAATCATGATTTCTTATGGTGCTAAAGCAATTAATTACCAAATTAATCCCCAACAAGCTCTTATGATTTCTGGATTGGTTGGAATTCAGTATTTAAAGGGTCAAAAAACAACTTTTACATTATATGTTTCATCCCAATTAGATATTCATCGTTGTAAATTAGAAAATTTTATAATGAATTTTAATAAACGATATTTATTAGCGAAGTTCAATTATCTAAATCAAGATCTTGAATTTGTTGACCATCTAATAACTTTAAATAAGGATCAAAAAACCAATATTTGTATTGCAGGCCTAGGTTTGTTAGTTATTAATGCTCATGCTGAACAAATATGTATCCGTTTACCAAAAAGTGTTGGTCTAAAAGTAGCAAAATATGCGATTATTTAA", "aa": "MITNKKCKGCGAFLNDQINTIGYTPKINETKTNLCQRCFKLIHYNKLEKVSTNLNQSIKETINTLNFSNLQIFAVLDILDLEHTIIDELKEYQNKIIFLVNKIDLLPQRYNSELVNENVVKTLINHGFIDPKIIYVSTHSNTSLKKVMDYIKNATNKKQKSIFFGKSNVGKSSLINALLALNKIKTKLTISSYTNTTINLNKINIQEHQILDAPGVCFDENILNYVRDEDNKSIMISYGAKAINYQINPQQALMISGLVGIQYLKGQKTTFTLYVSSQLDIHRCKLENFIMNFNKRYLLAKFNYLNQDLEFVDHLITLNKDQKTNICIAGLGLLVINAHAEQICIRLPKSVGLKVAKYAII", "aa_hexdigest": "636ad2c05d115dabb122241448c1e400", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1C7D", "ncbi_nrp_id": "WP_006688742.1", "uniref100_id": "UniRef100_A0AAC9T0H0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C7D", "RefSeq:WP_006688742.1", "UniRef:UniRef100_A0AAC9T0H0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T0H0", "uniref90_id": "UniRef90_A0AAC9T0H0" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T0H0", "gene": "yqeH", "product": "ribosome biogenesis GTPase YqeH", "uniref50_id": "UniRef50_B5ZBX1", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBX1" ], "product": "Ribosome biogenesis GTPase YqeH" }, "genes": [ "yqeH" ], "id": "GPKLONFDEH_945", "locus": "GCA000006625_00924" }, { "type": "cds", "sequence": "contig_1", "start": 535111, "stop": 535488, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01467.32" ], "nt": "ATGAAAATCATTTTGTTTTGTGGAGCTTTTGATATGGTTCACAATGCTCATATTGCAATGGCTAAACAAGCCATGAAGTTAGTTAATGCTGACAAACTAATTTTTTTACCATCAAATTTTAAATTTTTTAAAGCAATTAATAAAAATGACAATTTAGAGTATGAAAAAACAAAATTAACACCTGGACATCATCGTATAGCCATGTTAAAAATTGCAACCAAAAATTTAGTAAATATTGAAGTAAGTGACTACGAATTAAAACAAATTAATAAATCTTATACAATCAATACCATTGAGCATTTTAAACAAATATATGGTAGTGAACATGAATATTATTTTATTATGGGTAGTGATAACTTAGAACGTTTTAAACAATGA", "aa": "MKIILFCGAFDMVHNAHIAMAKQAMKLVNADKLIFLPSNFKFFKAINKNDNLEYEKTKLTPGHHRIAMLKIATKNLVNIEVSDYELKQINKSYTINTIEHFKQIYGSEHEYYFIMGSDNLERFKQ", "aa_hexdigest": "f0a820a00ea8809dfd90026957deaffd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01467.32", "name": "CTP_transf_like", "length": 119, "aa_cov": 0.952, "hmm_cov": 0.7899159663865546, "evalue": 1.72546997332958e-16, "score": 56.22269821166992, "start": 5, "stop": 123 } ], "seq_stats": { "molecular_weight": 14647.915199999985, "isoelectric_point": 9.11906909942627 }, "id": "GPKLONFDEH_946", "locus": "GCA000006625_00925" }, { "type": "cds", "sequence": "contig_1", "start": 535834, "stop": 535998, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTGTTGATTTAATGAATGTCTATGATAAAAAACTAATTGATCAAGCTTATTGTGCGGGAATTTATCATGACATACTAAAATGTTTAGATGAACAAGAAAGTGTTGCATATTTTAATGAACATAAAAGTGAATTAAATATTGGTGATGATTTTATCTCTTGA", "aa": "MCVDLMNVYDKKLIDQAYCAGIYHDILKCLDEQESVAYFNEHKSELNIGDDFIS", "aa_hexdigest": "940794005fd230a46159d33919558db3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6262.015399999998, "isoelectric_point": 4.295174980163575 }, "id": "GPKLONFDEH_947", "locus": "GCA000006625_00926" }, { "type": "cds", "sequence": "contig_1", "start": 536299, "stop": 536655, "strand": "+", "frame": 1, "gene": null, "product": "Purine nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C7E", "UniRef:UniRef100_Q9PQ20", "UniRef:UniRef50_Q9PQ20", "UniRef:UniRef90_Q9PQ20" ], "nt": "ATGATTGGTTTAATTGTGGCATTAAATAGTGAAATTAAAACCTTTTTTAAACAAATTAAAAAGAAGGTTTATCAAATTAATAATATTGATTTTTACTTGTGCACTCATCAAAATATAGAATTTGTTTTAGTTTTTACGGATGTAGGAAAAACTAATGCGAGTTTTATTACTGCATTATTAATTAATAATTTTAAACCTAAAGTGATTTTAAACGTTGGTAGTTGTGGTGCACTAAATGATCAGTTACAGGTTTTAGATATTGCCATTATTGATCAATGCCAATACTTAGATGTTAACGTTAGCGCTTTTGGTTATTTAAAAAATCAAATACCGCGTTTAGATAATTTTTTATTTTAG", "aa": "MIGLIVALNSEIKTFFKQIKKKVYQINNIDFYLCTHQNIEFVLVFTDVGKTNASFITALLINNFKPKVILNVGSCGALNDQLQVLDIAIIDQCQYLDVNVSAFGYLKNQIPRLDNFLF", "aa_hexdigest": "80fe2aedf8ce9bb9876cac0516771b03", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C7E", "uniref100_id": "UniRef100_Q9PQ20", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C7E", "UniRef:UniRef100_Q9PQ20" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ20", "uniref90_id": "UniRef90_Q9PQ20" }, "psc": { "uniref90_id": "UniRef90_Q9PQ20", "product": "Purine nucleoside phosphorylase", "uniref50_id": "UniRef50_Q9PQ20" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ20" ], "product": "Purine nucleoside phosphorylase" }, "genes": [], "id": "GPKLONFDEH_948", "locus": "GCA000006625_00927" }, { "type": "cds", "sequence": "contig_1", "start": 536799, "stop": 536939, "strand": "+", "frame": 3, "gene": null, "product": "MTA/SAH nucleosidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBW7", "UniRef:UniRef90_B5ZBW7" ], "nt": "ATGGAATTAGCAGCTATTGCTCATGTATGCACGCGTATGTTAACACCACTAGTAAGTATTAAATTAGTTAGTGATCATATTACTCTTCCCAATTCCAATCAAGAACAGTTTAATAAAAACTTATCACTAATTGATAAATGA", "aa": "MELAAIAHVCTRMLTPLVSIKLVSDHITLPNSNQEQFNKNLSLIDK", "aa_hexdigest": "629b629f4f97bfaa7deb8854afa07138", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 536784, "stop": 536957, "observations": { "mutation": [ 536937 ], "stop": [ 536937 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_B5ZBW7", "query_cov": 1.0, "subject_cov": 0.7666666666666667, "identity": 0.87, "score": 79.3, "evalue": 1.05e-18, "gene_start": 1, "gene_end": 46, "reference_start": 6, "reference_end": 51, "reference_sequence": "MDLVDMELAAIAHVCTRMLTPLVSIKMVSEHIMHPSSNQEQFNKNLVLIDKWFNKYLLEC" }, "paralog": false, "identity": 0.8245614035087719, "score": 95.5, "evalue": 5.81e-28, "description": "Internal stop codon at 536937. Nonsense mutation around 536937.", "psc": { "uniref90_id": "UniRef90_B5ZBW7", "product": "MTA/SAH nucleosidase", "uniref50_id": "UniRef50_B5ZBW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_B5ZBW7", "UniRef:UniRef50_B5ZBW7" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBW7" ], "product": "MTA/SAH nucleosidase" } }, "id": "GPKLONFDEH_949", "locus": "GCA000006625_00928" }, { "type": "cds", "sequence": "contig_1", "start": 537097, "stop": 537348, "strand": "+", "frame": 1, "gene": "ureE", "product": "UreE urease accessory N-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_004026601.1", "SO:0001217", "UniParc:UPI00000C1C80", "UniRef:UniRef100_A0AAX1QXP8", "UniRef:UniRef50_A0AAP9ADA3", "UniRef:UniRef90_A0AAP9ADA3" ], "nt": "ATGATTTATAAAGAAATTGTTCGTAATATTATCAAGCATCCATTAATCTTACCAGAAGGAAAACATTACCATTTACATAAAATTCTAACTTCAAAAGATGAATTACGTAAGGGTGAAGTTAGTTTAGTTGCAGAAAATGGCAAAGAATACACTGTTGATTTATCTGCATTTGTTGACTTATTAGTTGATGGTGATTGCATTATTGATGATAATCATTCACTTGTTGCTTTATACTTTGATGAAAGTAAATAA", "aa": "MIYKEIVRNIIKHPLILPEGKHYHLHKILTSKDELRKGEVSLVAENGKEYTVDLSAFVDLLVDGDCIIDDNHSLVALYFDESK", "aa_hexdigest": "bb146b024a5a55c70ea90580491ab26b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C1C80", "ncbi_nrp_id": "WP_004026601.1", "uniref100_id": "UniRef100_A0AAX1QXP8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C80", "RefSeq:WP_004026601.1", "UniRef:UniRef100_A0AAX1QXP8" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX1QXP8", "uniref90_id": "UniRef90_A0AAP9ADA3" }, "psc": { "uniref90_id": "UniRef90_A0AAP9ADA3", "product": "UreE urease accessory N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0AAP9ADA3" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ADA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ADA3" ], "product": "UreE urease accessory N-terminal domain-containing protein" }, "genes": [ "ureE" ], "id": "GPKLONFDEH_950", "locus": "GCA000006625_00929" }, { "type": "cds", "sequence": "contig_1", "start": 538148, "stop": 538489, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "GTGGTTGCTCAAATTAATGATAACAATGATTATATAATGCATTTTACGAATCCTAATTTAGTTAACAAGAAAATTAAATTAACATTTAAAGTTGATGATCAAGATGATCATACAAAAACCATTGAAGCTATCATTGGATTAGATGGAAAGGCTATTTTTAAAACAAGTGATGATGCAATTTTTGCACCAGATCATAAATATACACTAACAAAAATTGAAGCTAATAACAAAAAAGTTGCTAACATTGATGAAATTCCATTAAAAAATAGAATCGTTGATAAAAATCCAATTAAAAGAATTAACTTAACACAACTAAAAGTTGCTAAAGATTTAATTCTTTGA", "aa": "MVAQINDNNDYIMHFTNPNLVNKKIKLTFKVDDQDDHTKTIEAIIGLDGKAIFKTSDDAIFAPDHKYTLTKIEANNKKVANIDEIPLKNRIVDKNPIKRINLTQLKVAKDLIL", "aa_hexdigest": "2705eb6120084a9a946349d5c088e055", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 65, "aa_cov": 0.5752212389380531, "hmm_cov": 0.9230769230769231, "evalue": 1.9572948952007417e-14, "score": 49.374351501464844, "start": 17, "stop": 81 } ], "seq_stats": { "molecular_weight": 12884.755499999977, "isoelectric_point": 8.778804969787597 }, "id": "GPKLONFDEH_951", "locus": "GCA000006625_00930" }, { "type": "cds", "sequence": "contig_1", "start": 538598, "stop": 538813, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAATTGCATCACAACCTTTCAAATTAGAAAAAAATAAAACAACATACAACAATATCCTTTTGCCAATTAGTATTCCAAATCGCTTATATCATTTTAAAAAAGTAGTAATTCAAAAATTAAATCAACCAAATAAATCTATTGATTTAGATCATGATCCAAATTTAAAATCTTTTTTTATTGTTGGTCCTGGTAAAACAGATTTTGCATGA", "aa": "MKEIASQPFKLEKNKTTYNNILLPISIPNRLYHFKKVVIQKLNQPNKSIDLDHDPNLKSFFIVGPGKTDFA", "aa_hexdigest": "24408bc4b0876956c0e5549f5e99ad98", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8197.467299999998, "isoelectric_point": 9.698575401306151 }, "id": "GPKLONFDEH_952", "locus": "GCA000006625_00931" }, { "type": "cds", "sequence": "contig_1", "start": 538913, "stop": 539380, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTGGTTTAAATCAGATGCCGAACCAAATAATGATTCACTTAAATTTATAGGCGAATTATCTCAAACTAACGCCAATGGAACAGCTGCTGTCATTAATAATTTAAATAATTTATACGGTATGAAAGAACAAACAGAGTATTACATCTATAAAATTCAGTTCATAGATGAATTAGAATATTCAAAATTACCACCAAACCGTAATAACATTGTTTATGAATATAATAAAAATATTGCACGTAATTATGCATTCCGAACTAAAACTGCCCCTTTAATTTTAGAAAAAATAGAAAAAGATGATAATGGTAGCATTGGAGGACAAAATTTTTATTTTTACCTAAATAAAGCTAGTGATGATCCTAGTACAATCAAGCAAGTTACTATATATTGTCGTGATAATAATAATGAATTATATACTTCTGATGATCAAGAAATAGATTCTGTTGATCTTGTATATCATTCTTGA", "aa": "MVWFKSDAEPNNDSLKFIGELSQTNANGTAAVINNLNNLYGMKEQTEYYIYKIQFIDELEYSKLPPNRNNIVYEYNKNIARNYAFRTKTAPLILEKIEKDDNGSIGGQNFYFYLNKASDDPSTIKQVTIYCRDNNNELYTSDDQEIDSVDLVYHS", "aa_hexdigest": "c3de2f3c1fca8e2fb275b81623764956", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17984.64149999999, "isoelectric_point": 4.6213171005249025 }, "id": "GPKLONFDEH_953", "locus": "GCA000006625_00932" }, { "type": "cds", "sequence": "contig_1", "start": 539639, "stop": 539965, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACATTAGTTGAAGCTGAAAATCAAAACAATGCTGTTGTTGGTGACTTTTTACTTGCTGGCACTTCTATTAATAAAAATGATAGCCCAATTGGTGATAATAATGATAAGAACAAAGATATAGCAACTTTGAGCTGTAATTTTGAAAACAAATTAAAAAAAGGGACTAAATACTTATTAAAAGAAGTTCGATTTATTGCTAATCCAAATGAAGAAATTAGTGCTAAACCTACTAGAGCTGCTAGACCATTTAATAATGATAATATTATTTATAGTTTGAATCTACGAATAAATGATCCCTATGACTTTACAACTTTAAAAAATTAG", "aa": "MTLVEAENQNNAVVGDFLLAGTSINKNDSPIGDNNDKNKDIATLSCNFENKLKKGTKYLLKEVRFIANPNEEISAKPTRAARPFNNDNIIYSLNLRINDPYDFTTLKN", "aa_hexdigest": "12c61b19dca728c65abe18b9db80b5af", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12120.44679999998, "isoelectric_point": 6.235430717468262 }, "id": "GPKLONFDEH_954", "locus": "GCA000006625_00933" }, { "type": "cds", "sequence": "contig_1", "start": 540596, "stop": 541138, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "ATGGGAGAAGTTAGTTTTGATGTTTCACAATTAAAAAATAATAATTTATATGAGGTAATTGCTTTACAAACACAAGACCAAACTAAAATCGTTAATGTTAATGAGATTGAATACAACAATAAGATCATAAATAATTTAAATATTGATGAATTAAATACACCATACCAATATAGCAAAAATGGTCATCTTAACTTAATTGCTAAGATACCATTACGCTATGCTAATGAACAAGTCTATGGTGTTTTTGAAGACCAAAATAACCAAAAACATTTAATTCCTGCCGTAAATAAAAATGATGGAACAATAATGTTTGATACTCAAGTTTTAACTAAAAATCCCAATAAAAAATATCATTTACAAAAAGTTGTTTTAGCAAAAAATCAAAATCAAATTTTAATACATAATTTTGATTTAGTAAGTAATCAAAAACAAGATATCTTCTTAAAAGAATATAAAGTTATTAGTATTAGTGCTCTAGAAAATGATGCTGATAAAACTAAAAGAGAAATCAATTTAAAACTATCTAACATAAAAAATGACTGA", "aa": "MGEVSFDVSQLKNNNLYEVIALQTQDQTKIVNVNEIEYNNKIINNLNIDELNTPYQYSKNGHLNLIAKIPLRYANEQVYGVFEDQNNQKHLIPAVNKNDGTIMFDTQVLTKNPNKKYHLQKVVLAKNQNQILIHNFDLVSNQKQDIFLKEYKVISISALENDADKTKREINLKLSNIKND", "aa_hexdigest": "1d2c3711817f8194912b815bcb97197e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 60, "aa_cov": 0.3277777777777778, "hmm_cov": 0.95, "evalue": 9.848495410891498e-16, "score": 53.53261184692383, "start": 73, "stop": 131 } ], "seq_stats": { "molecular_weight": 20844.345900000015, "isoelectric_point": 6.912667274475097 }, "id": "GPKLONFDEH_955", "locus": "GCA000006625_00934" }, { "type": "cds", "sequence": "contig_1", "start": 541349, "stop": 542149, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGAAAGAACTAGTGCTGTATTAGCAACAAGTATTAGTGAAATAAGTAATCGTTTGGGAACTGCTTTAACGAATGCACAAGTTAAAATCACTTTAAAAGATGTGGATAATGTTTTATTAGCTAATCAAAAAGCGATTATTAACTATGGTAATAATCAAAAGAGTGAAGCAACTGTTATTGTTAACAATGGTGTTAAGTATCTAATAGCAACCTTAACTAAATTAACATTAAATACGCCTACAATAATTAAATCCATTACATTTGAACAAAAACCAACATGTGCTATTGAAAATATTGGGTTAGATAAAACAAATAACATTATTTATCAAAATACACATGAAGCAGCAATTCCTTTAGTAATTAATAATAATTTTGAAATCAATGGGCCATTAGCATCAAGTCATGAAAGTTTAAAAAGTATTAATGCATCTGATGCTAAAAAGGTAAATGCAATTAATTTAACCCTTGACTTTAATACCAATGAACATATTTATAAAAATTTATTTTTTAAACTAAAATATGTCCCTATTGACGATCAAGGTAATGAAAATATTTATGACGCATCAGAAGCAATTTATAGTGATGTTTTAAGTGCAAATAATATAATTGCTAATAAAATCCAATTTAGTTTAGGTAATTTACAAACTAATCGTAAATATAAATTAAAAACAATTTATTATTTAACATCAAAAACAACACCTTTAGATGATCATAACGTTGTAATGATCAAACCAAATGTTAAATCCGAAATTATTGTTGCCCCTAATGATAGTACGATTGTAAAATATGGTAATTGA", "aa": "MVERTSAVLATSISEISNRLGTALTNAQVKITLKDVDNVLLANQKAIINYGNNQKSEATVIVNNGVKYLIATLTKLTLNTPTIIKSITFEQKPTCAIENIGLDKTNNIIYQNTHEAAIPLVINNNFEINGPLASSHESLKSINASDAKKVNAINLTLDFNTNEHIYKNLFFKLKYVPIDDQGNENIYDASEAIYSDVLSANNIIANKIQFSLGNLQTNRKYKLKTIYYLTSKTTPLDDHNVVMIKPNVKSEIIVAPNDSTIVKYGN", "aa_hexdigest": "3709fd29decb7bbd2ede00dc96b3019c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29432.18290000003, "isoelectric_point": 8.408691978454595 }, "id": "GPKLONFDEH_956", "locus": "GCA000006625_00935" }, { "type": "cds", "sequence": "contig_1", "start": 542315, "stop": 542836, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGATTTTAATTTAAATAATTTAAAACCACAAACCACTTACCATTTAAAATCAATAACAATAGCAAAACCAAATAAAGCATATACTAATTTAAAAATTCAATCACTTCCACAAGTGGATATTAAAACGCAATTACCTAATTTAATCTTGCATGATTTAAAAGCCACAAGTGCGGTTTTAAAATGGGATTCTACTAACAATGGTAAAAACAATCACCAAACAATTCAAGCAACTTTTTCTCATGTAAGTGCTAATTATGATAATCTTAATGTTAAATTGGTTTATGAATATAATTATCGGGGTGATATCAAAGCAGTTGAATCTGATTTAATTACTCTTAAAAAAGACCAAAATGTTTATCAAATAAATTTACCAATCAGCGTTCCTAACCGAAAATATACTTTTAAGAAAGTTTTAATTCAAGAACGCAATAATTTAAACAACTATGTGGATTTAAATAAAAAGATAAACTTAACAGATTCATTTGTAGTTAGCCCTGGAAAAACAACATTTGAATGA", "aa": "MIDFNLNNLKPQTTYHLKSITIAKPNKAYTNLKIQSLPQVDIKTQLPNLILHDLKATSAVLKWDSTNNGKNNHQTIQATFSHVSANYDNLNVKLVYEYNYRGDIKAVESDLITLKKDQNVYQINLPISVPNRKYTFKKVLIQERNNLNNYVDLNKKINLTDSFVVSPGKTTFE", "aa_hexdigest": "5bf9a65c6a4405ada97e513e7dcae880", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19882.43800000001, "isoelectric_point": 9.522383308410646 }, "id": "GPKLONFDEH_957", "locus": "GCA000006625_00936" }, { "type": "cds", "sequence": "contig_1", "start": 543191, "stop": 543508, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "ATGAAAAATGCACCAACAATTTTACAATCAATAAAATTTGAAAATTGTGATGCTAATGGTGTAATTAATAATGAAGCGGAAGGAATTGCAGAATTTTCAATGACAACAAACAAGGTTAATGATGATTTTGCTAACCGAAAAGTTAAATTCATATATCATGACAATAACAATGTTGCTTATGAAACTGATGGTTTTCCATTAAGCAGATCAGAAACAAGTCTTCATTTTTCTTTATCAAACATCCCTAACAACCGCGACTATACTTTTGACCATGCTGAAATTGAAACTAGTCCTAATAGTAATAAATATGAAATTTGA", "aa": "MKNAPTILQSIKFENCDANGVINNEAEGIAEFSMTTNKVNDDFANRKVKFIYHDNNNVAYETDGFPLSRSETSLHFSLSNIPNNRDYTFDHAEIETSPNSNKYEI", "aa_hexdigest": "1fb2e9601c6d9502b64dc0e00c99d942", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 63, "aa_cov": 0.5904761904761905, "hmm_cov": 0.9523809523809523, "evalue": 1.032986702310715e-07, "score": 27.843162536621094, "start": 40, "stop": 101 } ], "seq_stats": { "molecular_weight": 11939.875199999988, "isoelectric_point": 4.757616996765137 }, "id": "GPKLONFDEH_958", "locus": "GCA000006625_00937" }, { "type": "cds", "sequence": "contig_1", "start": 543674, "stop": 543925, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAGTAGACGCACTCCAAACCGAAATTTCTAAGGAATTTTTACTTTCTGATGTTAAATTAGATTATCAAGATGGTAATATTGGAATATTAAAATGCCATTTTGATAACTTAATACCAAACACCGGATATACATTAAAAGAAATTAAATTTCTTGCTAACCCAAATGAACAACAAAGTGCAAAACCACATAAGGTAATTAAACCTTTTAATGATGATAACAATGTTATTATCGATAAAAATTATTCTTGA", "aa": "MSVDALQTEISKEFLLSDVKLDYQDGNIGILKCHFDNLIPNTGYTLKEIKFLANPNEQQSAKPHKVIKPFNDDNNVIIDKNYS", "aa_hexdigest": "07cbcaea9853e73f9fd8575479a54e6a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9453.585899999991, "isoelectric_point": 5.152136421203611 }, "id": "GPKLONFDEH_959", "locus": "GCA000006625_00938" }, { "type": "cds", "sequence": "contig_1", "start": 544036, "stop": 544941, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2", "UniRef:UniRef90_UPI0001794148" ], "nt": "ATGATTGCGTTAGAAAGTGAAAATAGTGTTAACAAATTAATCAGTATTATTGAATCATTACAACCAGATGATTATAATCCTTACTATAGTATTGAAAAACAAGAAAATAAGCAAAAAGATGAAAACAATTCTCAAGCAACATATAATGGTTTAGAACACCAAATTAAAAGTTTAAAATCAATCATTAACTTATTTGATTTTAACATTACTAATTTATCAATTGATGCAATCTATAATCTTAATGATGAATTAATTAAAAAATTTCAAAAAGCTAATTCACAAATTAATGATGTTGTGATTAAAATTAAACAAACCATTTTATCAAATTTTAAACAAATTTATTTATTTAAGTACTTCATTAAACATTTAAAAGAGTTTAATATTGAATTAAATAATGAAAACGATCCAATAAAAAAAGCTAATCGAATTCAAACCATTCAAGAAGTCTTAAAAAGTTATGAAAAGGATTTATTTTTAAATACTAAAATTAATTTAAAACTTGCTCCAAACGAAGTATTAACAGTAAATAATCTTGAAAAAATTGATTTAACATATGCTTCTGATTCGCTGCAAAAACCACAAGAATTAATCGCTTATTTAGAATTAAATAATAATCAAGAAAGAGAATTTAGCAAAGAACTTGAAAATCTTAGTGTTAGAGTTTTTACTATCATTGATAAAAAACTTTATATTCCCAAAGCTAAAGAATTAATTGATGAAAGCAAAAAAATTATTCAAACAAAAAATGAACATACAAATAATAAAGCAAAATTACAACGATTAGAAAAATTCACTAGTTTTGTTGAACAAGGAATAAGTAATGAAAATGTTTCTTCTTATACTCTTAATCTTTATATTCAAAGATTAACAAAACTGATTGAAGAAATTAAAAAACCACAGCATTAA", "aa": "MIALESENSVNKLISIIESLQPDDYNPYYSIEKQENKQKDENNSQATYNGLEHQIKSLKSIINLFDFNITNLSIDAIYNLNDELIKKFQKANSQINDVVIKIKQTILSNFKQIYLFKYFIKHLKEFNIELNNENDPIKKANRIQTIQEVLKSYEKDLFLNTKINLKLAPNEVLTVNNLEKIDLTYASDSLQKPQELIAYLELNNNQEREFSKELENLSVRVFTIIDKKLYIPKAKELIDESKKIIQTKNEHTNNKAKLQRLEKFTSFVEQGISNENVSSYTLNLYIQRLTKLIEEIKKPQH", "aa_hexdigest": "ab72d7e082040f8bd1c9736d19cae800", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 544036, "stop": 545241, "observations": { "mutation": [ 545097 ], "stop": [ 545097 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001794148", "query_cov": 1.0, "subject_cov": 0.48863636363636365, "identity": 0.96, "score": 545.0, "evalue": 1.85e-190, "gene_start": 1, "gene_end": 301, "reference_start": 316, "reference_end": 616, "reference_sequence": "MKYKKYQSIWLKISAVIVIPLLITMITACARIDKKQKSKEELQSSLNQAVLLKEIVDQSSYEEFKKRYNQTLKLNLANIDQLSESKLKEQKTIINNLISQVDILFELDAIKRINNLIDLNGLENVKKQLEDFLNQYEKLLYQTNVDMTIINSLKTEANQKKLDTKAIMQKTILDLVHQIKNLIKKSGSTKKIDELEADIQNLISDTPKDDHLKFYKDFKIIYKKLNDFNYWINKEIKAQPFIKKLDDIVNVINTIYEQNHHFLWFDQTKLTNLRQILIKNINHVLNVKKTLEIKEIKTLTNQLLLDLQNYLEGLKMIALESENSINKLISIIESLQPDDYNPYYSIEKQENKQKDENNSQATYNGLEHQIKSLKSIINLFNFNITNLSIDAIYNLNDELIKKFQKANSQINEVVIRIKQTILSNFKQTYLFKDFIKHLKEFNIALNNENDPIKKANRIQTIQEVLKSYEKDLFLNTKINLKLAPNEVLTVNNLEKIDLTYSSDLLQKPQELIAYLELNNNQEREFSKELENLSVRVFTIIDKKLYIPKAKELINESKKIIQTKNEHTNNKTKLQRLEKFTSFVEQGISNENVSSYTLNLYIQRLTKLIQEIKKPQH" }, "paralog": false, "identity": 0.9451371571072319, "score": 739.0, "evalue": 5.17e-269, "description": "Internal stop codon at 545097. Nonsense mutation around 545097.", "psc": { "uniref90_id": "UniRef90_UPI0001794148", "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001794148", "UniRef:UniRef50_A0AAC9X6L2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_960", "locus": "GCA000006625_00939" }, { "type": "cds", "sequence": "contig_1", "start": 545095, "stop": 545844, "strand": "-", "frame": 1, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2", "UniRef:UniRef90_Q9PQ15" ], "nt": "ATGATTAGTGCTATACCTTTGTTAGCAACAATAATTACAACTTGTGCAAAAGTTGATACAAAACAAAAAGCTAAAGAAGAATTACAATCAAGTTTAAATAAAGCTGTTTTATTAAAAGAAATAGTTGATCAATCATCTTATGAAGATTTTAAAAAGATATATAATCAAACTCTTAAATCAAATTTAGAAAACATTAATAAATTATCAGAGCAAAAATTAAAACAAGAACAACAAACAATTAAAAATTTAATTACACAAGTTAGTGTTTTAAATGAATTAGATTCAATCAAAAACATGAGTGATTTAATAGATGTTGTTGAATTAGAAAACACTAAAAAACAATTACAAGATTTTTTAAATCAATATGAAAAATTACTTAATCAAGCAAGTGTTGATACTACAATAATTGATAAACTAAAATCTGATGCTCATCAAAAAAGATTAGACACCAAAGCTTTAATTCAAAAAACAATTTTAGATTTAGCTAATAAAATTAAAAGCCTAATTAAAACATCTGGATTAAATAAAAACATTGTTGAGCTTGAAAATGATATTCAAAAATTAGTTAATAATGACACAAAAGCTAATTTTAAAAAACTATATGAAGATTTTAAATTAATTTACACTAAATTAAATGATATTAATGACTTAATTAACAAAGAACTAAAAGCTTTACCTTTTATTAAAAAATTAGATGATATAGTTAATGTTATTAATACTATTTATGAACAAAATCATCATTTTCTATGA", "aa": "MISAIPLLATIITTCAKVDTKQKAKEELQSSLNKAVLLKEIVDQSSYEDFKKIYNQTLKSNLENINKLSEQKLKQEQQTIKNLITQVSVLNELDSIKNMSDLIDVVELENTKKQLQDFLNQYEKLLNQASVDTTIIDKLKSDAHQKRLDTKALIQKTILDLANKIKSLIKTSGLNKNIVELENDIQKLVNNDTKANFKKLYEDFKLIYTKLNDINDLINKELKALPFIKKLDDIVNVINTIYEQNHHFL", "aa_hexdigest": "cf2f5f98d17c0c3ed64f029fcd7ee8aa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 544792, "stop": 545886, "observations": { "mutation": [ 545097, 545859 ], "stop": [ 545097, 545859 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ15", "query_cov": 1.0, "subject_cov": 0.4042207792207792, "identity": 0.996, "score": 452.0, "evalue": 7.52e-155, "gene_start": 1, "gene_end": 249, "reference_start": 15, "reference_end": 263, "reference_sequence": "MKHKKYKSIWLKLSMISAIPLLATIITACAKVDTKQKAKEELQSSLNKAVLLKEIVDQSSYEDFKKIYNQTLKSNLENINKLSEQKLKQEQQTIKNLITQVSVLNELDSIKNMSDLIDVVELENTKKQLQDFLNQYEKLLNQASVDTTIIDKLKSDAHQKRLDTKALIQKTILDLANKIKSLIKTSGLNKNIVELENDIQKLVNNDTKANFKKLYEDFKLIYTKLNDINDLINKELKALPFIKKLDDIVNVINTIYEQNHHFLWFDQTKLTNLRQILIKNINHVLNAKKTLEIKEIKTLTNQLLFDLQNYLEGLKMIALESENSVNKLISIIESLQPDDYNPYYSIEKQENKQKDENNSQATYNGLEHQIKSLKSIINLFDFNIANLSIDAIYNLNDELIKKFQKANSQINEVVIQIKQTILSNFKQTYLFKDFIKHLKEFNIELNNENDPIKKANRIQTIQEVLKSYEKDLFLNTKINLKLAPNEVLTVNNLEKIDLTYASDLLQKPQELIAYLELNNNQEREFSKELENLSVRVFTIIDKKLYIPKAKELINESKKIIQTKDEYTNNKTKLQRLEKFTSFVEQGISNENVSSYTLNLYIQRLTKLIQEIKKPQH" }, "paralog": false, "identity": 0.9862637362637363, "score": 686.0, "evalue": 6.35e-249, "description": "Internal stop codon at 545097, 545859. Nonsense mutation around 545097, 545859.", "psc": { "uniref90_id": "UniRef90_Q9PQ15", "product": "Unique hypothetical", "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ15", "UniRef:UniRef50_A0AAC9X6L2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6L2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6L2" ], "product": "Lipoprotein" } }, "id": "GPKLONFDEH_961", "locus": "GCA000006625_00940" }, { "type": "cds", "sequence": "contig_1", "start": 546042, "stop": 546764, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGATAATCATGAATTACTTTTTAAATTGCAAAATAATAAAAATTTACCTAATCAAAATGAAATTTTTATTCCCATTGAAAAATATAAAAAATGTGAAAACATGTTAGATTTAAATGTTTGTGATAAGTATGCATATAAAGATCTTAAAGAACAAAATCACTATTTTGATTCTTTAACTGAATCTAAATTTGCAAATCGTTTCCTTTCATTATCAAATGATAATGATAAAATTAAAAATAATGTTGAACTTTGGACTAAAAATCCTGTTTTTCATGGATTAAAATATCAATATTTTAATGAAGATGGTCAAATAAGTAATTCATACCCTGATTTTATTTTAAAAATAAAAACACATGATCAAAAAGATCATTATTTATATATTGAAGTAAAAAATTTATATAGTGATTATGATTATGAAAAAACAAAATTATTAATTGAATCATATCAACGATATATTGAAAATAAAAACAATAACGATAATTTAATATTAGATGAATTAGACAAAGCAGAAATTACGATTTTAGTTTGTTATATTAATATGAAAAATCAAAAAGATTTTTATTTTTATGGAGCTAGTTCAAATGATGTTTTAAATAAAAAAGTTAATTTTGATCTTTTAAAAAAGACAAGTATTAACAAAACTCCAGAACAACTAGTTGCGAATAAAGCAAAATTTATTATTGACATTAAACATTTATTAGATTTCTTACTTTAA", "aa": "MKKDNHELLFKLQNNKNLPNQNEIFIPIEKYKKCENMLDLNVCDKYAYKDLKEQNHYFDSLTESKFANRFLSLSNDNDKIKNNVELWTKNPVFHGLKYQYFNEDGQISNSYPDFILKIKTHDQKDHYLYIEVKNLYSDYDYEKTKLLIESYQRYIENKNNNDNLILDELDKAEITILVCYINMKNQKDFYFYGASSNDVLNKKVNFDLLKKTSINKTPEQLVANKAKFIIDIKHLLDFLL", "aa_hexdigest": "b51c29393ba179b07fcb882f11ba9247", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28749.355100000044, "isoelectric_point": 6.603122138977051 }, "id": "GPKLONFDEH_962", "locus": "GCA000006625_00941" }, { "type": "cds", "sequence": "contig_1", "start": 546870, "stop": 547394, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGTACAATTGCGAAACGTTTCTTCAAAAAATTTATCAGTACAAACAATTGGAAGAATTAAGCGTTTTCCAAATCCTAGCTTTGACAAAAACGAAATTAGCTATAATTCCATATCAAATCAATATTTTATTTATTCTAATATTATTTCAGAAGATAAAATTCGTCAAACTTTGATTTTAAAAGACAAATATAAAAATGATAGATTTTTTTATGGCGAAATTAATCAAGAGAAAATTAATAACTATTTAAATGGAGAAACTTATAAAGAAGAAATAGTAGAGAAGTTTGAAGATTCAATTAGTAATTTTGAATACTATGTTAATGAGGAATATCGTGAAAATTTTGTAAAAAATCAATACTTAGAAGGCGAAACTTCAAAAATTGAAGGTAAAACACGTGTTTATACTAAAATTAAAAATTCAATCGAACTTGAACTTTATATTATTGAACAATTAAGAAAATACAAACATCTATTTCCTCAAAAAATTATTGATTTTCTTGAAACGAAGTTTAATGAGTGA", "aa": "MLVQLRNVSSKNLSVQTIGRIKRFPNPSFDKNEISYNSISNQYFIYSNIISEDKIRQTLILKDKYKNDRFFYGEINQEKINNYLNGETYKEEIVEKFEDSISNFEYYVNEEYRENFVKNQYLEGETSKIEGKTRVYTKIKNSIELELYIIEQLRKYKHLFPQKIIDFLETKFNE", "aa_hexdigest": "32f18eab0a314898152afc414a2c8689", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21086.53980000002, "isoelectric_point": 6.659620094299317 }, "id": "GPKLONFDEH_963", "locus": "GCA000006625_00942" }, { "type": "cds", "sequence": "contig_1", "start": 547557, "stop": 548519, "strand": "-", "frame": 2, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "nt": "ATGAAATTAACCAAATCACAACAAAAAGCTGTTAGTCAATTAGTTAATTGTTATTTAGAAAGCGTGAATAACAATAAAAAAGAAATTATTGATTTTCAAGCACCAACAGGATCTGGTAAAACCTTTATTATTACCAATACTATTAATGAAATTATTAAGACAAACAAAGCTAATGGTAGTCCTCAAAAATTAATTTTTGTAATTGCGACTTTATCATCAGCTGATTTACCATTTCAATTAGAACAAAATATGAATGAGTATAAATATTATATTAATGGTTTATTTGATGTCGAACTTAAAGAATCACCATCTTCTAAAAGTGCTAAAAAAGATAGTTCTTACAATATTTTAGCTGAAGAAAATAAAGTAATTATTTTTGGGTCTTCTTCATTTGGTAAAGGAAGAATTATTACAGAAGAAGGAATTTTAGATGCTTTTTTAGATGAAATTAAATCACAAGGATATACATTAGTTTATATCCGTGATGAAGCTCATCATGGCGGTAATGTTAATAAAACAAAAATATTTGAAGATTTTGATGAAAAAATACACAAAAATTCTTTTAAAAAAGAAGAAACACGATTTGAATATAAAATTCAAGAAGCAGCATCTTTTATTATTAAAATGACAGCAACTCCTAAAGGAATTCATAAATTAGTATTCATTGATGAAAAAGATTTAATGGATGATGATACAAAATTGTTAAAAGATGAACATGTTTATAATCTAGAAATTAAAGAACTAAAAGAAGAAAACATTGATGATGAACTATTATTAACAACTGCTTGTGAGCAATTTTTAAAAATCAAAGAGCAATATTCAAAAGCAAAAGAGTTAAAAAATATTCGTCCAGCCATGTTAATTCAAGTTGAAAATGAACCTAATAAAGAACCACAAAAAAGCGAATTTTTAGATAAAATTGAAAAAATTATTAAAATTTTAGAAAAACATAATTTATCATGA", "aa": "MKLTKSQQKAVSQLVNCYLESVNNNKKEIIDFQAPTGSGKTFIITNTINEIIKTNKANGSPQKLIFVIATLSSADLPFQLEQNMNEYKYYINGLFDVELKESPSSKSAKKDSSYNILAEENKVIIFGSSSFGKGRIITEEGILDAFLDEIKSQGYTLVYIRDEAHHGGNVNKTKIFEDFDEKIHKNSFKKEETRFEYKIQEAASFIIKMTATPKGIHKLVFIDEKDLMDDDTKLLKDEHVYNLEIKELKEENIDDELLLTTACEQFLKIKEQYSKAKELKNIRPAMLIQVENEPNKEPQKSEFLDKIEKIIKILEKHNLS", "aa_hexdigest": "1218b70d4ae05e5a7f50960e64d809a0", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0001981E5C", "query_cov": 1.0, "subject_cov": 0.9815950920245399, "identity": 0.8690000000000001, "score": 533.0, "evalue": 1.6e-189, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_UPI001E2BAF28" }, "pscc": { "uniref50_id": "UniRef50_UPI001E2BAF28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "GPKLONFDEH_964", "locus": "GCA000006625_00943" }, { "type": "cds", "sequence": "contig_1", "start": 548525, "stop": 548632, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGTTGATTTTAATAATACAAGTCAATCAAGTTTATTTGAAAATAACCAACGCGAATTATTAATTAATTTAAGTACACTAAAACCAATTGAAAAAAAGGAATAA", "aa": "MLVDFNNTSQSSLFENNQRELLINLSTLKPIEKKE", "aa_hexdigest": "f7370372e60cb772f8f0f2df5938564f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4094.600300000001, "isoelectric_point": 5.155035209655761 }, "id": "GPKLONFDEH_965", "locus": "GCA000006625_00944" }, { "type": "cds", "sequence": "contig_1", "start": 548744, "stop": 548974, "strand": "-", "frame": 3, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF32C8C", "UniRef:UniRef90_UPI0015EFB420" ], "nt": "ATGCATTTATTAGATTTACATTCCAATAAAAATGCTCGTGTTTTAGATTTCTTCGCAGGATCTGGAACAACTGGTCATGCAGTATTAGAATTAAATAAAGAAGATGGTGGAAATCGAACATTCACATTAATAACAAACAATGAAAATCAAATAGGCACTAATGTTTGTTATGAACGCTTATATCGTATCAACAATGGTATTGGAACTAAAAACGAAATCGATTTTGATTGA", "aa": "MHLLDLHSNKNARVLDFFAGSGTTGHAVLELNKEDGGNRTFTLITNNENQIGTNVCYERLYRINNGIGTKNEIDFD", "aa_hexdigest": "3daa0b799e7f98c4b74033cdedc7c4f3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 548519, "stop": 548995, "observations": { "mutation": [ 548527, 548746 ], "stop": [ 548527, 548746 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015EFB420", "query_cov": 0.9868421052631579, "subject_cov": 0.47468354430379744, "identity": 0.8, "score": 131.0, "evalue": 1.16e-37, "gene_start": 2, "gene_end": 76, "reference_start": 9, "reference_end": 83, "reference_sequence": "PKPIELIKYLINIHPNPNARVLDFFAGSGTTGHAVLDLNKEDGGNRTFTLVTNNENNIGIDVCYERLYRINNGKGSKGEVDFDWIKKNEPYNQNLDVFEINYCDTDVLANNNEIIKKDFKNMLNDFNLSNVNKINEQEILLNLTALKPIEGKDSNESN" }, "paralog": false, "identity": 0.6139240506329114, "score": 202.0, "evalue": 5.88e-68, "description": "Internal stop codon at 548527, 548746. Nonsense mutation around 548527, 548746.", "psc": { "uniref90_id": "UniRef90_UPI0015EFB420", "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI002FF32C8C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015EFB420", "UniRef:UniRef50_UPI002FF32C8C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FF32C8C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF32C8C" ], "product": "DNA methyltransferase" } }, "id": "GPKLONFDEH_966", "locus": "GCA000006625_00945" }, { "type": "cds", "sequence": "contig_1", "start": 549194, "stop": 549604, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01555.25" ], "nt": "ATGAACGAACGTTTAAAATTAGCTAAAAACTTACTTAAAAATGATGGTGTAATTTTTGTATCCATTGACGATAATGAACAAGCATACTTAAAAGTTTTGATGGATGAAATTTTTGGCGAAGAGAATTTTGTTACAAATTTTATTTGGCAAAAGAAATCTGGTGGTGGTTTGAATAAATTAATATATGAAGGCCATGAGTATATATTATGTTATTCAAAAAATAATTACAATTTTTGTCTTTCTGAAAAAAATAAAAAAATGAGTGGTTTTATTAAATATAAAGATAAAAACAATAATTCTTTTTTTATAAATAGCGATATTATTAGAAATAATTTTGGAAAAAAAGATAATAAATTCGAACATAGAAACAAAGCTTATGAAGATTTATCTAAAGAAGATAAAGAAAAATGA", "aa": "MNERLKLAKNLLKNDGVIFVSIDDNEQAYLKVLMDEIFGEENFVTNFIWQKKSGGGLNKLIYEGHEYILCYSKNNYNFCLSEKNKKMSGFIKYKDKNNNSFFINSDIIRNNFGKKDNKFEHRNKAYEDLSKEDKEK", "aa_hexdigest": "7014a12c7028bb6f9149a53e6f11443d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01555.25", "name": "N6_N4_Mtase", "length": 128, "aa_cov": 0.9117647058823529, "hmm_cov": 0.8125, "evalue": 2.0827059049949324e-16, "score": 56.12824630737305, "start": 1, "stop": 124 } ], "seq_stats": { "molecular_weight": 16102.065799999986, "isoelectric_point": 8.704666328430175 }, "id": "GPKLONFDEH_967", "locus": "GCA000006625_00946" }, { "type": "cds", "sequence": "contig_1", "start": 549762, "stop": 550112, "strand": "-", "frame": 2, "gene": null, "product": "Type III restriction-modification system: methylase", "db_xrefs": [ "RefSeq:WP_010891792.1", "SO:0001217", "UniParc:UPI00000C1C86", "UniRef:UniRef100_A0A2C9DYR5", "UniRef:UniRef50_A0A2C9DYR5", "UniRef:UniRef90_A0A2C9DYR5" ], "nt": "ATGATTAAAGATTTAAATAGTTATTTAAAAGACATTGATGAAATGAGTAAGAGTTCATTAAATGAGGATCAAAAACAATTAATTAAAAAAATTTTAGAAAAAACTGATCCCCAGGATTTAGATAATGTGTTTCAATTATTAATTCAACGTGTTAAAATAGGATTTAGTTTTGATGTTGCTCCAAGCGTGCACCAAACTCAAATTGCGATATTAGCTAAAAATGAGCAATTATCTTTCATGAATGATGCACATAATTTATTTAAAAATGATACTAATAGTTTAATCATTGGTGAAAACTATGATGTACTAAAGAATTTATTAGTGTTAGAGAGAGAGAGAGAGAGAGACTAG", "aa": "MIKDLNSYLKDIDEMSKSSLNEDQKQLIKKILEKTDPQDLDNVFQLLIQRVKIGFSFDVAPSVHQTQIAILAKNEQLSFMNDAHNLFKNDTNSLIIGENYDVLKNLLVLERERERD", "aa_hexdigest": "e759ea6bf515278b551bd4eef37d0e51", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C86", "ncbi_nrp_id": "WP_010891792.1", "uniref100_id": "UniRef100_A0A2C9DYR5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C86", "RefSeq:WP_010891792.1", "UniRef:UniRef100_A0A2C9DYR5" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYR5", "uniref90_id": "UniRef90_A0A2C9DYR5" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DYR5", "product": "Type III restriction-modification system: methylase", "uniref50_id": "UniRef50_A0A2C9DYR5" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYR5" ], "product": "Type III restriction-modification system: methylase" }, "genes": [], "id": "GPKLONFDEH_968", "locus": "GCA000006625_00947" }, { "type": "cds", "sequence": "contig_1", "start": 550797, "stop": 550958, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTATAGCAATGATATTGGTAGTAGTGATCAAATAATTAATAGAGAAAATCTTTTATTTAAATTAAAAAACCGTATGTATGTTAAAATTCCATTACCAATTGCTAATCGAGAATATAATTTTGAACGAATAGAAGTTTTAAACGATGATAATACTTGA", "aa": "MAYSNDIGSSDQIINRENLLFKLKNRMYVKIPLPIANREYNFERIEVLNDDNT", "aa_hexdigest": "621aece6aeb15c1991a474d28a26680a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6290.0587, "isoelectric_point": 5.135198402404785 }, "id": "GPKLONFDEH_969", "locus": "GCA000006625_00948" }, { "type": "cds", "sequence": "contig_1", "start": 551025, "stop": 551291, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGAATTTAATTTAAAGTTTGATGACATTAAAACTGCTACATTATATGAACTAGATAATATTTATTATTTAGATAAAAATAATCCAAATAATTTAGATGAAAAACATAAATTAAATCAAATTACTTATAATTTTGAATTACAAAATCCTTTATCAATAGCTTTGGTGGAAAAATATCTAAAAAACACACTTGATGTTGATAAAAAAACTAATCATCCAGTTTGTGAAGTGAAATTATTTATTTTAAATGTTAAATATCCTTGA", "aa": "MIEFNLKFDDIKTATLYELDNIYYLDKNNPNNLDEKHKLNQITYNFELQNPLSIALVEKYLKNTLDVDKKTNHPVCEVKLFILNVKYP", "aa_hexdigest": "af453acf2aa7df6eeabe5b7510f15ff9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10478.91069999999, "isoelectric_point": 5.587125205993652 }, "id": "GPKLONFDEH_970", "locus": "GCA000006625_00949" }, { "type": "cds", "sequence": "contig_1", "start": 551292, "stop": 552671, "strand": "-", "frame": 2, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYB1", "UniRef:UniRef90_A0A2C9DYB1" ], "nt": "TTGCAACTAAAAGATTTAAATAATCAAATTTATAGTATTGATGCTAAAGTAGATGTAGAAGGAAATGTTGATTTTGATGTAAGTAAATTAGCAAATAATAACTTCTATGAATTAGTTAACATTTATAAAAATAACACGCATCCTTTAGTTAAAGTTATTAATCCAGTTAATATCGCATATCATAATAAAACTATTAACAATTTAAATGTTAATAGTTTAAACCAACCATATCAGTATACAAAAAATGGTGATATTAATTTAATTGCTAAAGTAGCGCCATATTATGTTAACCAACAAGTATATGGGATTTTTAAAGATCAAAATCATAAATTACATAAATTATTAGCTAAAGTTGATAATGATGGTCAGATTTGTTTTGATATTGGAAAATTAGTAAAACCAAACCAGTATCGATTAGAAAAAATTGTTTCTGCTAGTGATGAAAATATTGAATTCGTACACAATTTTGATTTATCTAGCAATCAAAAACAAACTCTTAATAGACCAAATATAGTGGTTAATTATTTAAAAAACGGGGATTATACAATAAATTTTAACGATGCAAATTTAATTAATAAAAAGATTGAACTTAGATTCAAAATTGATAATACCAATGAAATAAAAACAATACAAGCAATAGTAGATGTTAATAAAAAAATAACACTAAAGACAAATGATAATACTTTATTTGCGCCAAATCATAAATATACATTAATTGGAATTGTTGATAATAAACTAACAGTCAATAACAATATTGCGAATTTAGATGATATTTTAGAAATTAACAAAGTGATTATTAAAAAACCCTTAGTTAAAGTTTTAACAAGTTCACTAGTTGAAGAACCGCGATCACCAAATAGTATTAATTCAACAAATATAAAGTTTGAAATTAGTGATGAAGCCAATGTTTTAAATACAAATATGAGTGCAACTATTAATTATGATGATAATAAAATTACAAAAGCTCATGTAGTGGCTGAAAATAATAAAAAATATTTAACAGCTCGATTAGATAATTTAGAATTAAATAAAACATCTTTTATTAATAAAATTGAATTTGATCAAAAGCCGCCTAAAGCAATTGTAAATATTGGTTATTTAGATACTAATATTATTTATGATGATACAAAAGAAGAAAAAAATCAACCTTTGACAATCAATAATAATTTTGCATTATTACCATTAGAAAACCAAGAAGATTATTTTCAACGTAAAAATGAAGCTAATATTAAATTAAAAGTTGAATTAAAAGCCAACTCTAATATTCTAAAAAACCTTACATTTGCTGCAACTTTTAAATACAAAGACAACAAGATTGTCAAAAAAATTAAAACTAACGCTATTAGTTGTACACCAATTAAAAATGAAATAAATAAGTGA", "aa": "MQLKDLNNQIYSIDAKVDVEGNVDFDVSKLANNNFYELVNIYKNNTHPLVKVINPVNIAYHNKTINNLNVNSLNQPYQYTKNGDINLIAKVAPYYVNQQVYGIFKDQNHKLHKLLAKVDNDGQICFDIGKLVKPNQYRLEKIVSASDENIEFVHNFDLSSNQKQTLNRPNIVVNYLKNGDYTINFNDANLINKKIELRFKIDNTNEIKTIQAIVDVNKKITLKTNDNTLFAPNHKYTLIGIVDNKLTVNNNIANLDDILEINKVIIKKPLVKVLTSSLVEEPRSPNSINSTNIKFEISDEANVLNTNMSATINYDDNKITKAHVVAENNKKYLTARLDNLELNKTSFINKIEFDQKPPKAIVNIGYLDTNIIYDDTKEEKNQPLTINNNFALLPLENQEDYFQRKNEANIKLKVELKANSNILKNLTFAATFKYKDNKIVKKIKTNAISCTPIKNEINK", "aa_hexdigest": "fa7f9d87ca86bf7e7d91815e14548469", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 550989, "stop": 552971, "observations": { "mutation": [ 551027, 551294, 552695 ], "stop": [ 551027, 551294, 552695 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYB1", "query_cov": 1.0, "subject_cov": 0.4469328140214216, "identity": 0.961, "score": 850.0, "evalue": 1.47e-301, "gene_start": 1, "gene_end": 459, "reference_start": 225, "reference_end": 683, "reference_sequence": "MKMKNNKKIHWKKHIKIASFLICLNLCWSAVIIGIVLRFYDKENSIIFVDQNNDSYWLKIHDKKDLKTEINNHLPQGNVYRLQKNKLSNSIIGLTLSKIDKTNEYSNTGDIVIKVNLKNKAKKNPQIMGVFVDEKHQYHYIQPTIKNKVAYFNTSKLANNHDYRLVKILDKTDYNHVLIQQNELAIEHQIMISKPPRTTSFINHNNQKIYRINVGKWLFNSSLSLQLKDLNNQIYSIDAKVDAEGNADFDVSKLANNNFYELVNIYKNNTHPLVKVINPVNIAYHNKTINNLNVNSLNQPYQYTKNGDINLIAKVAPYYVNQQVYGIFKDQNHKLHKLLTKVDNDGQICFDIGKLVKPNQYRLEKIVSASDENIELAHNFDLSSNQKQTLNRPNIVVNYFKNGDYTINFNDANLINKKIKLKFKIDNTNEIKTIQAIVDVNKKITLKTNDNTLFSPNHKYTLIGIVDNKLTINNNIANLDDILEINKVIIKKPLVKVLTSSLVEEPRSANSINSTNIKFEISDEANVLNTSMSATINYDNNKITKAHVVVENNKKYLTARLDNLELNKTSFINKIEFDQKPPKAIVNIGYLDTNIIYDDTKEEKNQPLAINNDFALLPLKNQEDYFQRKNEANIKLKVELKANSNILKNLTFAATFKYKDNKIVKKIKTNAISCTPIKNEINKWMIEFNLKFDDIKTATLYELDNIYYLDKNNPNNLDEKHKLNQITYNFELQNPLSIALVEKYLKNTLDVDKKTNHPVCEVKLLILNVEYPWLVNKKARFVFSYTTVDGTKKEFMAYSNDIGSSDQIINRENLLFKLKNRMYVKIPLPIANREYNFERIEVLNDDNTWTIFNQSYRTIRCRPSQNTILTTTSDFNENKENKYIKNISPTQVNLNLELISHDQAFHDGLVANLELIDDKGNIKGPYQITLSKTDKEFDGSIKNHIDPNKHQELQTGLTSWLISENKINNLEPNTKYYINRIYFSEKPNYLTYKWNFANDNNEIYNRNQAKKDNPQHNDYSFTTTKLI" }, "paralog": false, "identity": 0.9606060606060606, "score": 1269.0, "evalue": 0.0, "description": "Internal stop codon at 551027, 551294, 552695. Nonsense mutation around 551027, 551294, 552695.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYB1", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYB1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYB1", "UniRef:UniRef50_A0A2C9DYB1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYB1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYB1" ], "product": "DUF1410 domain-containing protein" } }, "id": "GPKLONFDEH_971", "locus": "GCA000006625_00950" }, { "type": "cds", "sequence": "contig_1", "start": 552693, "stop": 553085, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGTCTAATTCCATAATTGGTTTAACTTTATCTAAGATAGATAAAACCAATGAATATTCAAATACTGGTGATATTGTTATTAAAGTTAATTTAAAAAACAAAGCTAAAAAGAATCCGCAAATTATGGGTGTTTTTGTTGATGAAAAGCATCAATATCATTACATACAACCAACTATTAAAGATAAAGTTGCTTATTTTAATACATCAAAATTAGCAAATAATCATGATTATCGTTTAGTAAAAATTCTTGACAAAACTGATTACAATCACGTTTTAATTCAACAAAATGAGTTAGCGATTGAACATCAAATTATGATTAGTAAACCACCTAAAACAGCTTCTTTTATTAACCAAAATAATCAAAAAATATACCGAATAAATGTAGGAAAGTGA", "aa": "MSNSIIGLTLSKIDKTNEYSNTGDIVIKVNLKNKAKKNPQIMGVFVDEKHQYHYIQPTIKDKVAYFNTSKLANNHDYRLVKILDKTDYNHVLIQQNELAIEHQIMISKPPKTASFINQNNQKIYRINVGK", "aa_hexdigest": "a9b168fa9177a13b7be6b96b1772cbc9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 60, "aa_cov": 0.45384615384615384, "hmm_cov": 1.0, "evalue": 6.133632154965003e-16, "score": 54.19129180908203, "start": 33, "stop": 91 } ], "seq_stats": { "molecular_weight": 15029.128799999986, "isoelectric_point": 9.61425075531006 }, "id": "GPKLONFDEH_972", "locus": "GCA000006625_00951" }, { "type": "cds", "sequence": "contig_1", "start": 553654, "stop": 553845, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAAAAAACATATTAAAAAAATAGTAATTGGAGCTTTAAGTACACTAGCGTTCATCACAACAACAGTTGTAGCAACAGCTTGTGCAAAACACAATAATTCTGTTACTAAAAATAATGAACCAAACGTTAATGTTAAAGATGAAATAAGTTCTTCTAATAGTGAATTAAATGATTCACATTATTATTGA", "aa": "MIKKHIKKIVIGALSTLAFITTTVVATACAKHNNSVTKNNEPNVNVKDEISSSNSELNDSHYY", "aa_hexdigest": "3585722c2447dca50b572a1d52b18c06", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6903.760900000001, "isoelectric_point": 8.854555320739745 }, "id": "GPKLONFDEH_973", "locus": "GCA000006625_00952" }, { "type": "cds", "sequence": "contig_1", "start": 553897, "stop": 554250, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCGAAGATGGAAAACGTGCATTTTTATTACCAGGGTTTTCACAAAGCAATTATCTACAAGACGCAATAAAAAACGCTAAATTTGATAAAAATGCTGTTGGTTTTTTATTAGATACAGTTTATAATGCAAATAATGATCCTTCTTTATTTGATGGTGCAAATCGTGTTGCAAGTGTTTATTTTAAAGTTGATGATGCTGCTTTTTTAGGAGGGATTGCTGCCGCTTATATGCTAAATGCAAACCAAAACATTTTTGCAACAGATGGCCAACTTAACTGGGGAGGATATGTTGCTTTAAATGCTAAAAACACTACTAATTATTTAGCAGGATTTGCCTTGGGTATTGAATGA", "aa": "MVEDGKRAFLLPGFSQSNYLQDAIKNAKFDKNAVGFLLDTVYNANNDPSLFDGANRVASVYFKVDDAAFLGGIAAAYMLNANQNIFATDGQLNWGGYVALNAKNTTNYLAGFALGIE", "aa_hexdigest": "9fdfdb1799e6fe29e4cbab56f882e960", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12613.953899999986, "isoelectric_point": 4.677076148986816 }, "id": "GPKLONFDEH_974", "locus": "GCA000006625_00953" }, { "type": "cds", "sequence": "contig_1", "start": 554449, "stop": 555117, "strand": "+", "frame": 1, "gene": "med", "product": "Conserved hypothetical ATP/GTP-binding protein", "db_xrefs": [ "COG:COG1744", "COG:T", "SO:0001217", "UniRef:UniRef50_Q9PQ10", "UniRef:UniRef90_Q9PQ10" ], "nt": "ATGAGTTTAGCAGTTAATGAAGCAATTAGCACAACTTCACACAAAGTAGCAATAATAGGCGTAGATACTGAACAAGAAAACGATAAGACAATTAATAAAGAAGCAAATACTTTTAAAAATACTAATATAAGTGGAAATAAAAATGGCGTTATTCGTTTTTCAATTGTTAAACGATTAGGAGTAGCAATTGAAAAATTACTAGAAAACGCTGTAAATGGTACTCAATTTCAAGGACATGTTAACAAAATTGGTGATGAAATTGATGTTCACGATAAATATAAATTAGGTGTAAACACTGTAGGTTCACTAGTTGATGGTGTAGTAGGAATCTCAGAATCTGCTTATCACTATTTAATTGATGCTTTTAATCTAGCACAAACAGATGAAACTAATAAAGTAAGTACATATGACCAATTAGTTCAAAAAATAATCCAAGATCCTTTATTTAAAAGTTTAAACCAAGATCCTAAAATTGATGGATATATAAACCCTAAAAATCTTGCTAACGCTAGGGGTAATGAAGGTGAATTAATAAATGGAAAAACAATTAGTCCAGAAGCAAATGGGGGAACCATTTATAAAGCAACAAATGGTTCATATTATTACTATCCTGTTGCTAAATCAACATATACTGCACAAAATGCCTCAACTATTTTTAAAGAAATATGA", "aa": "MSLAVNEAISTTSHKVAIIGVDTEQENDKTINKEANTFKNTNISGNKNGVIRFSIVKRLGVAIEKLLENAVNGTQFQGHVNKIGDEIDVHDKYKLGVNTVGSLVDGVVGISESAYHYLIDAFNLAQTDETNKVSTYDQLVQKIIQDPLFKSLNQDPKIDGYINPKNLANARGNEGELINGKTISPEANGGTIYKATNGSYYYYPVAKSTYTAQNASTIFKEI", "aa_hexdigest": "1e8dff0cdb00f680d89d1d17c828f3de", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "med" ], "truncated": "both", "pseudogene": { "start": 554149, "stop": 555273, "observations": { "mutation": [ 554248, 554311, 555115 ], "stop": [ 554248, 554311, 555115 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ10", "query_cov": 1.0, "subject_cov": 0.41187384044526903, "identity": 0.977, "score": 424.0, "evalue": 3.56e-145, "gene_start": 1, "gene_end": 222, "reference_start": 266, "reference_end": 487, "reference_sequence": "MIKKHIKKIVIGALSTLAFITTTVVTTACAKHNNSVTKNNEPNVNVKNEISSSNSELNDSHYYWKAPKSDTSEGFQTIHKSMVEDGKRAFLLPGFSQSNYLQDAIKNAKFDKNAVGFLLDTVYNANNDPSLFDGANRVASVYFKVDDAAFLGGIAAAYMLNANQNIFATDGQLNWGGYVALNAKNTTNYLAGFALGIEWANKHLANKKVLQEGSSESKTWIKVNEVQASQSSAGGFDENNENAKKILKELISKKADLILPVAITQMSLAVNEAISTTSHKVAIIGVDTEQENDKTINKEANTFKNTNISGNKNGVIRFSIVKRLGVAIEKLLENAVNGAQFQGHVNKIGDEIDVHDKYKLGVNTVGGLVDGVVGISESAYHYLIDAFNLAQTDETNKVSTYDQLVQKMIQDPLFKSLNQDPKIDGYINPKNLANARGNEGELINGKTISPEVNGGTIYKAANGSYYYYPVAKSTYTAQNASTIFKEIWDKAKNDDDKRRLVGLILSFAGANVKDGGYSEISYDGMLGFYAKHGVKIPKL" }, "paralog": false, "identity": 0.9732620320855615, "score": 716.0, "evalue": 1.36e-262, "description": "Internal stop codon at 554248, 554311, 555115. Nonsense mutation around 554248, 554311, 555115.", "psc": { "uniref90_id": "UniRef90_Q9PQ10", "gene": "Med", "product": "Conserved hypothetical ATP/GTP-binding protein", "uniref50_id": "UniRef50_Q9PQ10", "cog_id": "COG1744", "cog_category": "T", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ10", "UniRef:UniRef50_Q9PQ10", "COG:COG1744", "COG:T" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ10" ], "product": "Conserved hypothetical ATP/GTP-binding protein" } }, "id": "GPKLONFDEH_975", "locus": "GCA000006625_00954" }, { "type": "cds", "sequence": "contig_1", "start": 555683, "stop": 555877, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATCAAAATGATTCTATTTTTGGTGAAGTTATGTACAAAAATTTAGATAATGGTCAAATTTATAATACAACAGTTGGAATAATTGAAAATAATGTTAACAAAAATTATAATACTCCTGGCCAATTTCAACTACTTTTAGATAAAATGCCAAAATCTTCAAGATATCAATTTTATGGTATTAAATATTGA", "aa": "MNNQNDSIFGEVMYKNLDNGQIYNTTVGIIENNVNKNYNTPGQFQLLLDKMPKSSRYQFYGIKY", "aa_hexdigest": "fc731ecca39d7e90d097874f216844d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7488.317800000003, "isoelectric_point": 7.960765647888184 }, "id": "GPKLONFDEH_976", "locus": "GCA000006625_00955" }, { "type": "cds", "sequence": "contig_1", "start": 556058, "stop": 556252, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATTTATCAACCAATAAAATTGATGATAATCCAATAATTAAAATTGTAAATGACAATGGTAAACCTAAATTACAAATTCATATTAACAATATTAATATAAATGATCAAAATAATGAATATAAATTAAGAATTGTAAAATTTGAAAATAAACCAAATTCTTACCTATATAAAAACATAAATAATGATTGA", "aa": "MENLSTNKIDDNPIIKIVNDNGKPKLQIHINNININDQNNEYKLRIVKFENKPNSYLYKNINND", "aa_hexdigest": "7969c29b2a63777e41e702dcc4b21663", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7555.4109, "isoelectric_point": 7.98513469696045 }, "id": "GPKLONFDEH_977", "locus": "GCA000006625_00956" }, { "type": "cds", "sequence": "contig_1", "start": 556337, "stop": 556987, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATACAAATTCTAAATTCCAATTGGTATTTAAAAAAGTTAGTGATAGTACCAAGACATTTATACAAACAATTAATTTAGTAAGAAAAGATAATAAATGGATTCTTGAAGGAGAAATATCTAATTTAGAGCCAAATACAGAATACATTTTATCTACAATAAAATTATCAAGACCAACAGGCAATGATCTATTTGATACAGAATTAAACACAAACATTAACATTATTGAACAAAATCAAAATAAAATTTTAACTAAAGGTATTGATCCATATATCGAATCAATTACTTATGAAAAAACTAATGATTTAAGAAGAAGAATTATTAAATTAAAAATTAAGGATATAAAAGATTACAATGATGGTGATAGATTAGTATTAGAGTATAGATACAATAATTCAACTTCTAAAAAAGAAGTAAGCTTAACTTTAAATAAAAATCAAGAAGATTATAGCGTTAATATTGAAAATTTAACTTATAATCGTGAATATACTTTAAAAAATATTATTAAAATAAATCATAATACTAATAATATTACTTCAATAGATATTAGTAAATTAAATGATAAAACATTTGTTGTTGAACCAAGTGTGACAAAATTAACTAATTTTAAAAATATTACAAATTTTGATGAAAAAAAACAAATTTGA", "aa": "MLNTNSKFQLVFKKVSDSTKTFIQTINLVRKDNKWILEGEISNLEPNTEYILSTIKLSRPTGNDLFDTELNTNINIIEQNQNKILTKGIDPYIESITYEKTNDLRRRIIKLKIKDIKDYNDGDRLVLEYRYNNSTSKKEVSLTLNKNQEDYSVNIENLTYNREYTLKNIIKINHNTNNITSIDISKLNDKTFVVEPSVTKLTNFKNITNFDEKKQI", "aa_hexdigest": "15e37deed7b318f99f95b4ea03bebfa9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25322.378400000038, "isoelectric_point": 8.986393165588382 }, "id": "GPKLONFDEH_978", "locus": "GCA000006625_00957" }, { "type": "cds", "sequence": "contig_1", "start": 557084, "stop": 557314, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTTTATACAATGTCAATCATTGATTCAAGAAATGATGGATTATACGCAAATATTAAACATGATTTAGTAAATCTTGATGAAGGAAGTGAATATTACATAAAAGAAATAAGAATGATTAAAAAACCAAACAAAGCTTATGAAGATGTAAATGGTAATAAAATTATTTCACCAATTAATGATGCACAAAGAAGAACGTTAGAGAATACTAATTTTAAAACAAATTGA", "aa": "MKVYTMSIIDSRNDGLYANIKHDLVNLDEGSEYYIKEIRMIKKPNKAYEDVNGNKIISPINDAQRRTLENTNFKTN", "aa_hexdigest": "cf1f706708bd419cec3dd551df311149", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8866.937799999992, "isoelectric_point": 7.947356224060059 }, "id": "GPKLONFDEH_979", "locus": "GCA000006625_00958" }, { "type": "cds", "sequence": "contig_1", "start": 557666, "stop": 557914, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAATAAATAAAAATAATGTAATATATGGAGGTAATCTAGATACTAAATTCAAAACATTAGTTGATAAACTAAAATTATTGAAATTAGAATCAAATAATGATATTTATTACAATATACAAGAAAATGCTGTTAATCCTAATAGAGTGATAGGAACAATAAAAGCAGAATTTTCAAGAATTAATTCTAATTACATTAACAAAAAAATTAAATTAGTATATGAAGATAACAATCATCAAGAATGA", "aa": "MLTINKNNVIYGGNLDTKFKTLVDKLKLLKLESNNDIYYNIQENAVNPNRVIGTIKAEFSRINSNYINKKIKLVYEDNNHQE", "aa_hexdigest": "51ee4085a5231ecc1ce66d4fb1231147", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9559.782199999998, "isoelectric_point": 9.188114738464353 }, "id": "GPKLONFDEH_980", "locus": "GCA000006625_00959" }, { "type": "cds", "sequence": "contig_1", "start": 557996, "stop": 558382, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAATAATAAAAAGATTTACGATTTAGTTCTTGATAAGGATTTGATTACTAATCGTGAATATAAATTATTTAAAGCTCAAATTACTGATTCAAAAGATACTAATACAGTGTTTAATGATTTAAGTATTAATGGTGTTAGTCATAAATTTGGTATTAATCCTTATAATACAAATTTATCTATCGATTATTCTAATTGGGTAACAAATTTAACTGAGAATAGTGCTACTATAAATGTAAAAGTTAATTCAACTGATCAAGTTTTTGAAAATAATCAAATTGTTGAAGCTACATTTGTATCTACATCAAATCCATCAGAAATTAAAAAAATTGATGGTGTATTAAGTGAAGTAAGTGCAAATAAAAATAGTGCTAAAATTAAATGA", "aa": "MENNKKIYDLVLDKDLITNREYKLFKAQITDSKDTNTVFNDLSINGVSHKFGINPYNTNLSIDYSNWVTNLTENSATINVKVNSTDQVFENNQIVEATFVSTSNPSEIKKIDGVLSEVSANKNSAKIK", "aa_hexdigest": "1bb84d6ecdb438c5f46f5fb70ff032d2", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14380.826499999988, "isoelectric_point": 5.355278968811036 }, "id": "GPKLONFDEH_981", "locus": "GCA000006625_00960" }, { "type": "cds", "sequence": "contig_1", "start": 558662, "stop": 558826, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCAAATATTTGAAACTAATCAATCAGTTATCGTATATCTTGAAAATAAAAATAATGGTCAAATAAGTCAATGGACTAGAAAAATTAGTGAAGTTAATTCTAATAAAACTGTAGCTACAATCAATGTTGAAACAGGGGCCACATTAAATGAGCAAACTTAA", "aa": "MNQIFETNQSVIVYLENKNNGQISQWTRKISEVNSNKTVATINVETGATLNEQT", "aa_hexdigest": "8e280a4c82ed40f8a79936721f28ab15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6099.663300000002, "isoelectric_point": 5.062103462219238 }, "id": "GPKLONFDEH_982", "locus": "GCA000006625_00961" }, { "type": "cds", "sequence": "contig_1", "start": 558810, "stop": 559211, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATCAACAAATGAAATTAATGTTGATTCTCAAACAATTAATGCAACATTTTCTGGAGTAAATGATCAATATAATGGGCGTCGAGTAAAATTAATTTATCGTGATCAAAATAACGAATTATATGAAAGCAATATAGTTAATTTAAAAAAAGGTATGCAAAAATATAATTTTACTCTAAGTGGTCTGATAGCTAATAGAAATTACACTTTTATTAAAATACAAATTAATGATGATAAAAATAATTCTCAATATATTGATTTAAAAAAACAAAATACTGTAAATAATAGTTTTAAACGAAACCCATCAACAACAAGCATCGAATGGAGCACTCCATTAAATATTACGTATAATAGTGCTGAGTTAAATATAAAATTAAAATCTGTTGAATCAAATATTTGA", "aa": "MLSTNEINVDSQTINATFSGVNDQYNGRRVKLIYRDQNNELYESNIVNLKKGMQKYNFTLSGLIANRNYTFIKIQINDDKNNSQYIDLKKQNTVNNSFKRNPSTTSIEWSTPLNITYNSAELNIKLKSVESNI", "aa_hexdigest": "a69b173a57b315624fb8ee48f1ffab3b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15350.945199999984, "isoelectric_point": 9.300160789489745 }, "id": "GPKLONFDEH_983", "locus": "GCA000006625_00962" }, { "type": "cds", "sequence": "contig_1", "start": 559297, "stop": 559590, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGGTTTTGTAATATTAATTGAATTTAATACGTATGTTGAATTTGGTTCTAAATTATTTATTTTACCTTTAATTAATCATTTCCCATTTTCTTTTAATAAGTTAACATTTACTTGTTTTAAAGTACCACCATTTTTATTAAATGTTACCGTTGCTGTTGCATTTGCATCTAATTCTCCATCATTATCATCTGTTTTTAGAAGAAAATTAAATTCAGCACTTGTATCTGAAGGATTTGATCATTCGCCATTTTTAGAAATTATAGTGTGTGATTTGTTTATTTTAATTTGA", "aa": "MLGFVILIEFNTYVEFGSKLFILPLINHFPFSFNKLTFTCFKVPPFLLNVTVAVAFASNSPSLSSVFRRKLNSALVSEGFDHSPFLEIIVCDLFILI", "aa_hexdigest": "755a4bd5c7bef642d6a0a27668470a5d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10951.854399999991, "isoelectric_point": 6.701055717468263 }, "id": "GPKLONFDEH_984", "locus": "GCA000006625_00963" }, { "type": "cds", "sequence": "contig_1", "start": 559539, "stop": 560411, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATAATTTAAAACGTAATCGGACATATACCTTAATTGATGTAAAATTGATTGATAATAATAATGTTTCAGATTTCCCAAAAGAAGGTAATTTAACAAATAGTTTTATTACAACAAGAACAAGCGCCATAAATGTATTGAATATTGAAGAAATTAGTAATCGAGCCTCAACTAATTTAAAATCTACTATTATTAAAATTAATTTAAACGATCCAGATAATGTACTTAGAGATAAAGATCAAGCTACTATTGTTTATGGTAATAATAAACAAGCAATGGGATTTATTACTGTAAGTGGTAATATCAAATATTTAACTGCTACTTTAGTTGATTTAAATTTTAATGATAAAGTAAATATAGTAAATATTAGTTTTAATAATAAACCAAGTATAGCAGCTGAAAATATTGGAATTGATAAATCTAATAATATCATTTATAATAATGATTCAATACCTAAATTAGAAATTAATAACGATATTATTGTTAATGGTCCAATAAATAAAGAAATAGTTGTTAAAAACGCTAATCAAAAAAACAATATCGATGTAGATCTTGGATTGCAAATTAATCCTAAAATAGCACATAATTTAAGATTTATAGCTAAATTCAAATCAACAAATAATGATATTATTGAAACTAATGTTATTAATGGGTCATCTTTAGTAAATAATGACGGAAAGACATCAATTAGATTTACTTTAAATAATTTAAAAGCTAATAAGTTATATTCATTAGTTGATGTGTATTATTTAGTAAATAATAATTCAAATACAATTGTTGAAAGTAATAAATTACCAAAATTAAATAACATTAATTATCAAATTAAAATAAACAAATCACACACTATAATTTCTAAAAATGGCGAATGA", "aa": "MLNNLKRNRTYTLIDVKLIDNNNVSDFPKEGNLTNSFITTRTSAINVLNIEEISNRASTNLKSTIIKINLNDPDNVLRDKDQATIVYGNNKQAMGFITVSGNIKYLTATLVDLNFNDKVNIVNISFNNKPSIAAENIGIDKSNNIIYNNDSIPKLEINNDIIVNGPINKEIVVKNANQKNNIDVDLGLQINPKIAHNLRFIAKFKSTNNDIIETNVINGSSLVNNDGKTSIRFTLNNLKANKLYSLVDVYYLVNNNSNTIVESNKLPKLNNINYQIKINKSHTIISKNGE", "aa_hexdigest": "ba5091a6c5b3fa80160cedf0047f1de3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 32446.36050000001, "isoelectric_point": 9.33020305633545 }, "id": "GPKLONFDEH_985", "locus": "GCA000006625_00964" }, { "type": "cds", "sequence": "contig_1", "start": 560988, "stop": 561140, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATCAAATTTAAATTCAAATCGAGAATATAGGTTTGAAAAAATTGAAATAAATCATATTTCTAATACTAATAATTTTGAAGATCTTGAAAAATTAAATGGTGTGTCAAATACATTTATTACTCAAACAAAAAATACTACAGTTCAATGA", "aa": "MLSNLNSNREYRFEKIEINHISNTNNFEDLEKLNGVSNTFITQTKNTTVQ", "aa_hexdigest": "b0a759585804535f69a0d3e7ac059ddb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5861.382000000001, "isoelectric_point": 5.625434684753418 }, "id": "GPKLONFDEH_986", "locus": "GCA000006625_00965" }, { "type": "cds", "sequence": "contig_1", "start": 562623, "stop": 563375, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATATAATAATAAAACTAATGTAAAAGTTAATTTAGAATTAAAAGCTAATGATAATATTTTGCAAAATTTATACTTTATAGCAACATTTAAAGATCAAAATAATGAAACCGTAAAATCAAGTATTGTTTCTTATAGTTCAATCAAAAAAGAAGGTAAAAAACAAATTATTGAACTTAGAATTCCAAATCTAAAATCAAATCGTTTATATCACTTTGATAATTTATATTATTTATTAAATCAAGACGATAAAGATCTTATAGATAATAATAAATTAATAAGATCTAATAATGTTGTTAATGAAATTGAAGTTAAACCCGGTAATACAACTTTTAAAAAAAATAATGTTGAATGGCAGATCAATTCAAACAGTGTAAATTTTAAATTACAAGTTGAATCTGATGATGAAGACATATTAGATAATAATGTTATGGCTGATGTTATCTTTTCATCAAAATCAAATCAAAATGATACAAAATTTGTTAACAATGTAAAATTAAAAAAAGAAGGAAACATTTGGTTTATTGAAGGTATAGTAAATAATTTAAAACCAGAAACAGAGTATGAGTTAAAGTCTATTGTATTAACTAAGCCACTAAATGCTAACTCTAATCTTAAAACTATTAATCCAACTAATATTTCATTTAAAACAAAACCTGGTAATTATGGAATTATAAATATTGAATCAAATCAAACACTAACAAATTCGCAAAATGTAAATGTTACAATTGATGGAATTCGTAGTGAATGA", "aa": "MEYNNKTNVKVNLELKANDNILQNLYFIATFKDQNNETVKSSIVSYSSIKKEGKKQIIELRIPNLKSNRLYHFDNLYYLLNQDDKDLIDNNKLIRSNNVVNEIEVKPGNTTFKKNNVEWQINSNSVNFKLQVESDDEDILDNNVMADVIFSSKSNQNDTKFVNNVKLKKEGNIWFIEGIVNNLKPETEYELKSIVLTKPLNANSNLKTINPTNISFKTKPGNYGIINIESNQTLTNSQNVNVTIDGIRSE", "aa_hexdigest": "e9ae701fdc033715c9b8dcada798d587", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28697.885100000043, "isoelectric_point": 6.654788780212404 }, "id": "GPKLONFDEH_987", "locus": "GCA000006625_00966" }, { "type": "cds", "sequence": "contig_1", "start": 563796, "stop": 563990, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTATATACTTCAAATGACGGTGAAGAAATTTTAAGTGATCAAAAAACATTGCTTTGGGCTAATAACCACTATAGTTTCGAATTGTCTAATTTAAAACATAATCGTAAATATACACTAAAAGAAGTAAGAATTATTAATGATGATAATAAAACATCAATAATTTTCCATTTAAAAAATGGAATAGCTGATTGA", "aa": "MVYTSNDGEEILSDQKTLLWANNHYSFELSNLKHNRKYTLKEVRIINDDNKTSIIFHLKNGIAD", "aa_hexdigest": "9ad9f8dc886d03fed9d5f6803a9113c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7510.325799999998, "isoelectric_point": 6.254187583923341 }, "id": "GPKLONFDEH_988", "locus": "GCA000006625_00967" }, { "type": "cds", "sequence": "contig_1", "start": 564009, "stop": 564353, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATCTAATGATGATGAACGTCAAGAAATATCATATACAAAAACTATCACAAGTATAAGTAGTGATGGAAGTGAGGCAACAGTTAGTTTTAATACTGATCAAACACTAAAAGAAGAAGCAAATTATAAACTAATAAAAGTTGGCTTTGTTAGAAAACCAACATTAGCTTACACTAATATTAATAATGATGCTAATAATGTCATTTTTGAAGACAATAATTCGAATTATAATTTTAAAACCTTAATTGTTGATCATAAAGTAACAAATGTTAGTTCTAATGATTCTATAAATACTACAACACAAACAGTTAATATTGATATTGATGGAATTCAACGAACTTGA", "aa": "MKSNDDERQEISYTKTITSISSDGSEATVSFNTDQTLKEEANYKLIKVGFVRKPTLAYTNINNDANNVIFEDNNSNYNFKTLIVDHKVTNVSSNDSINTTTQTVNIDIDGIQRT", "aa_hexdigest": "44ebdae37a0f3aae71bd6d35430e8831", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12802.791999999981, "isoelectric_point": 4.699982261657714 }, "id": "GPKLONFDEH_989", "locus": "GCA000006625_00968" }, { "type": "cds", "sequence": "contig_1", "start": 564427, "stop": 564582, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGTTATATTTGTTGCTAAATTAGTATTATCAATGACTATTTGTGTCTTTGATGCATTTACATTAAAACTATTCGTAACATTTGTTGAATTATTAAGTGTTTCAAAATTATTATTAGAAGTCTTTATTTTTATTTCTTTAAAAGAATATTGA", "aa": "MLVIFVAKLVLSMTICVFDAFTLKLFVTFVELLSVSKLLLEVFIFISLKEY", "aa_hexdigest": "495d915e53329712fcf0ed9ec8b0e914", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5876.247299999996, "isoelectric_point": 6.010859870910643 }, "id": "GPKLONFDEH_990", "locus": "GCA000006625_00969" }, { "type": "cds", "sequence": "contig_1", "start": 564786, "stop": 564950, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CED52", "UniRef:UniRef90_UPI00215CED52" ], "nt": "TTGGCTTATAATGATACATACAAATTAGATGACATCTCAATTACTAAACCAATGAATGCTTTTGCTAATTTAAAAATCCAAATTGAAAATAAAGAACAAATTAGTTTTACAACTCAAAGTGGACCAACACAATTAGTTAGCATTACATCTCATGATTTAATGTGA", "aa": "MAYNDTYKLDDISITKPMNAFANLKIQIENKEQISFTTQSGPTQLVSITSHDLM", "aa_hexdigest": "d464093f8052c35d7122480750636072", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 564729, "stop": 564971, "observations": { "mutation": [ 564788 ], "stop": [ 564788 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215CED52", "query_cov": 0.9814814814814815, "subject_cov": 0.6625, "identity": 0.9059999999999999, "score": 99.8, "evalue": 2.06e-26, "gene_start": 1, "gene_end": 53, "reference_start": 8, "reference_end": 60, "reference_sequence": "IEDTISDLAYNDTYKLDDISITKPINAFADLKIQIQDKEQISFTTQSGPTQLVSITSHDLTWNDQPNASNQQTITAKVSG" }, "paralog": false, "identity": 0.925, "score": 147.0, "evalue": 1.02e-47, "description": "Internal stop codon at 564788. Nonsense mutation around 564788.", "psc": { "uniref90_id": "UniRef90_UPI00215CED52", "uniref50_id": "UniRef50_UPI00215CED52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215CED52", "UniRef:UniRef50_UPI00215CED52" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CED52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CED52" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6106.8629, "isoelectric_point": 4.889369773864746 }, "id": "GPKLONFDEH_991", "locus": "GCA000006625_00970" }, { "type": "cds", "sequence": "contig_1", "start": 566496, "stop": 567035, "strand": "-", "frame": 2, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CB5C8", "UniRef:UniRef90_UPI00215CB5C8" ], "nt": "TTGGTATTTAATAAAAATGTAATTATTAAAAAAATTGAATTTAAAAATTTATCTCAAACCTTTATTAAAGTTGGCAAAAATAATACAAATGTAATTTATGACGAAAGTAATTTAAATAAATTAATTATTAATAATGATTTCCAAATCATTGGTCCATTATCAACTGATGTTAATAGTACACAAAATATAGTGGCTAATAATAAACACGTAATTTCTTCAACACTAGATTTTAAAGTTAATCCACATATTAGTAAGAATTTGAAATTTAAACTTAAATTTCAAAATATCAATGGTGAAGTTGTTTATAGTCCAATTTTAACTAATAGTTCAATTATAGTTAATAACAATAAAAACGTAATTAATTTTACTTTAGATAATTTAAAATCAAATCAATTATATAGATTAGTTGATGTTTATTATATTGATGATAATAATGATACTATTAATGACAAAAACAAAGTACCAAAAGCAAATAATGTTACTCGAATAATTGACATTGCACCTGGTAAAACAACAATTAGCAAATCAAATAATACTTGA", "aa": "MVFNKNVIIKKIEFKNLSQTFIKVGKNNTNVIYDESNLNKLIINNDFQIIGPLSTDVNSTQNIVANNKHVISSTLDFKVNPHISKNLKFKLKFQNINGEVVYSPILTNSSIIVNNNKNVINFTLDNLKSNQLYRLVDVYYIDDNNDTINDKNKVPKANNVTRIIDIAPGKTTISKSNNT", "aa_hexdigest": "f3d54c6f94787c9f0b5d2c8856af90cc", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 566253, "stop": 566975, "observations": { "mutation": [ 566498 ], "stop": [ 566498 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215CB5C8", "query_cov": 0.88268156424581, "subject_cov": 0.6556016597510373, "identity": 0.9179999999999999, "score": 276.0, "evalue": 5.02e-92, "gene_start": 21, "gene_end": 178, "reference_start": 1, "reference_end": 158, "reference_sequence": "FTKVGKNNTNVIYDENNLNKLIINNDFQIVGPLSTDANNTQNIVANNKHVISSTLDFKVNPHISKNLKFKLKFQNMNGEVVYSPILTNSSIIVNNNKNVINFTLANLKSNQLYRLVDVYYIDDNNDTINDKNKVPKANDVTRIINIAADKTTITRSNNNWNSTATSSQFEFEVSSEDGNDVLENLKATIIFKKDQTLLTPIEVNITKENDKYLIKGEITNLEPENTYTLESIVLAKPSKTN" }, "paralog": false, "identity": 0.85, "score": 412.0, "evalue": 3.88e-148, "description": "Internal stop codon at 566498. Nonsense mutation around 566498.", "psc": { "uniref90_id": "UniRef90_UPI00215CB5C8", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_UPI00215CB5C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215CB5C8", "UniRef:UniRef50_UPI00215CB5C8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CB5C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CB5C8" ], "product": "DUF1410 domain-containing protein" } }, "id": "GPKLONFDEH_992", "locus": "GCA000006625_00971" }, { "type": "cds", "sequence": "contig_1", "start": 567504, "stop": 568073, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTATTTTGATGATCAAAATTCAGTGAATGATATGACAAATAATCAAATTAGTTTTAATTCAAAGATTCATAAACCAAAAATTGAATTTGAACCATCATTAACAACAATTAATTATGATACTAATAATGCTATTAAAACAGTTAGTGCACATAATGCTCAAGTACATTTTAAGCTTAAAACAAATGATGAAGCATTAGAAAACGATCAAATTGTCGAAGCAGTTTTTGCTCCAACTAATAATTTAAATGATCAAAAAGTAGTTGAAGCAAAATTAAATAATGTAACTAATTCATTTAATGAAGGTGAATTAGAATTTAATTTAAGCGGTTTAAGAGAAGAAACGACATATCGTTTAATCAAAGTAACATTTAAAAATAAACCAAATAAAGCATATGAGTTATTAAATAAAAATGGTGTTATTTTTGAGTATAAAAATGGTTCACAAGCATATGAATTCACGACACAAAAATTTGAACATAAAGTTATTGATGTAGTGTCAAGCACATCAACAAATACAACACAACAAGAAATTACTGTTAAAATTGATGGTATTCAGCGAGCTTGA", "aa": "MYYFDDQNSVNDMTNNQISFNSKIHKPKIEFEPSLTTINYDTNNAIKTVSAHNAQVHFKLKTNDEALENDQIVEAVFAPTNNLNDQKVVEAKLNNVTNSFNEGELEFNLSGLREETTYRLIKVTFKNKPNKAYELLNKNGVIFEYKNGSQAYEFTTQKFEHKVIDVVSSTSTNTTQQEITVKIDGIQRA", "aa_hexdigest": "05152376307e67019c803b93b77cfd9e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21625.718100000006, "isoelectric_point": 5.427066612243652 }, "id": "GPKLONFDEH_993", "locus": "GCA000006625_00972" }, { "type": "cds", "sequence": "contig_1", "start": 568986, "stop": 569678, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTGTTAAAACTAATAATGATACAACTATCACCTTTGAAATTGATGATAAAGATAATATTTTAAAAAATGATCAAAAAATCTATGCACAGTTAGCTTTAATGGATGATGATTTATTAGATACAGATGTTATTAACCCTTTAGTTAATTTTAGTAAAACTAATAAAATCGCTTCAATTAATGGTCTTGATTTAAAAGGAAATAGTAAATATAGTATTAAAAACTTATATTATTTAAATGACCAAAACCAAAAGGTGTATTTATTTAAAAATGATGTTACAAAATATGAACAACATTTTATTACAAACCCTCATAAGATCAATTTATCATTTAATAAAAGCGCTGTAGAACAAGATATTTTTGCAGACCATGCTAATTTATTTATTGATTATAAAGATTATGATCAAAAATTACGAATTAATGAAGATGTTAAAATTTATTATCAAAATATTGATAACACTAAAAACGAACTACAAATTGGATATGGAAAAGTTGTTGCAAATAATAAAATTAAATTCAATTTGGTTGGTTTAAAAGAAAAAACAACATATGTTATTAAAAAACTTGAAGCTTTAAATAAGAGTGCAAGCAGTATTGTTAATAGTGAATTTGATTTACTAGATCCAAATACTAATTTTAGTACATCTAATAAAAACACAACACTAGTGGGTCTAAATTCAATTGATAATTGA", "aa": "MVVKTNNDTTITFEIDDKDNILKNDQKIYAQLALMDDDLLDTDVINPLVNFSKTNKIASINGLDLKGNSKYSIKNLYYLNDQNQKVYLFKNDVTKYEQHFITNPHKINLSFNKSAVEQDIFADHANLFIDYKDYDQKLRINEDVKIYYQNIDNTKNELQIGYGKVVANNKIKFNLVGLKEKTTYVIKKLEALNKSASSIVNSEFDLLDPNTNFSTSNKNTTLVGLNSIDN", "aa_hexdigest": "1a370eb39601f15c81bc51c5c946f6f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26398.376300000036, "isoelectric_point": 5.66175479888916 }, "id": "GPKLONFDEH_994", "locus": "GCA000006625_00973" }, { "type": "cds", "sequence": "contig_1", "start": 569691, "stop": 572063, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGCCAACAGCAGTTTTTAAAGATGAAAATAATAAAGAATATTTAATACCAGCGTTGAGTGTAAAAAATAATGTTGGGTATTTTAATACTAAAAAACTACCTTTAAATCACAAATATGAATTAAACCGAATTGTTAACAATAATGAATTAAATGATGTTTTAGTGCCAAATAATGAATTAATGCTTGAACATAAAGTTAGTGTAAATAAACCAACAAAAGCTAAAGTTAATTTTATTGATAACAAAAAAGTATATGAAGTTGATTTAGGTGTTCAATTAAAAAACACTCCTCTTGAACTTACCTTAGAAGATCTAAATCATCAAACATATAAAATTAATGCAAAAACAGATGAAAAAGGACGAGCTGTTTTTGATATTTCATCTTTAGGTGATAATAATTTATATGAAGTTATTGGTATTAAAAAAACTAATGAAGTTGATGTTGTTAATCTTAAACAGATTCCATACCATAATCGATCAATTAATAATTTAAATTCAAACGCATTAAATACCCCATATCAATATACAAAAAATGGTGATATTAATTTAATTGCTAAAGTAGCGCCATATTATGTTAACCAACAAGTATATGGAATTTTTAAAGATCAAAATAATCAAGAACACCAAATTTTAGCAAAAGTTAAAAAAGATGGAACCATAGCATTTGATACAGGTGCATTAAATAATAACAGTAATTATTCACTAGACAAAATTGTTTCAGTTTCAAACCCACAAAATGTTTTGGTTTCAAATTTTGATTTAACTTCAAAACAAAAACAATTAATTAAAAAGCCAGCTGCTAATGCAAGTGTTGATTCAACTAAAAAAACCCAAATTTTAGAAAATTTAAATAATGATTTAATTAATCAGAAATTAGTAGCAACTTTTGTTGATAATAATGATAAAGAATATAAAGTAGTTGCTAATGTGGATCAAAATAATAAAATTATTTTTGATAGTAATGATTTACCAAAAGGATATATTTATCATTTAGCTAAAGTTGAAAATAACGATTTAAATAAAGTCATTAATCTTAATGATTTTGAACTAAAAGATAAAATAATTGATAAACGAGATTTGAATTTATTAGATTCACATCCAGATTTTACGTACGATAATGATGGAAATTTAGAAATTCACACTCAATTAGCAAATGATTTAAATGATGATTTAAAACAAAAAGCTTTAAATAATGCAAATGTCAAAGGAATTGTTGTTGATCAAGATGGTATAGAACATGAGATAGATGTAAGTATTGATGCTAATGGCAAGGTTATTATTCCAACAAAAAATTTAGCAAATAATGACCCAACTAAACCTAATATATACACACTTAAAAAAGTCGTTTTAAAACAAAATAATCAACCAAATATTGATCTAATTAGTGAAGAGCAATTAAGTGGTGATAATCATATTTCATTTAAAAAACCAACAATAACTGCTAAAACTAAAGAAAATGATGATTATGAAATTAGTTTTTCAAATCCTAGTTTAGCAAATAAAAAAATTAAATTAACATTTAAAACCGATGATAATAATACTAATACAAAAACGGTTGAAGCTAGTATTGGTTTAGATGGAAAAGCTATTTTTAAAACAAGTGATGATGCAATTTTTGCACCAGATCATAAATATACACTAACAAAAATTGAAGCTGATAATAAAAAAGTTGCTAATATTGATGAAATTTCACCATTAGATCGAATAGTTAATAAACAAAAAAATGGTAATGTTAATGCTGATAATAAGCATGAGTTTAAAATACCAGATCAAAAAAATAAAGATTTAACTGCTGTTTATAAAGATAAAAATAACAATGAAATTCATGTTCCAATCAAAACTGATGATAAGGGAAAAGTTATTGTTAATCCAAACAATAATTTATTTGATCCTAACAAAATCTATGATTTTGATAAGATTGTAGATTTAAATGAATATCCAAACAAAACAATATTAGATAGAAATAGTATTAACAAAGATGTTAGTGCAATTAATGATGGTGTCGATCAAGCTCGTAAGCTTGTTTTAAAAGCACCAGCAGTTTCTAACGTAACAGTAAATGCTATTAATTTCCAAGTAAACTTATTTGATTTAAATAAACTATCTTATAATCGTCAATTTGCACTAACAATTGCTAAAGTTAATGATTTAAATGATACACAAAAATACATTGCAACTTATGATCCAAAAACTAACAACTATAAACTAAATTTTGATTTTACACACTTAGATGCAAACACAAAATATAAAGTTGTTGATGTTGAATTACTAGAATTAAACAACAAAGAAAAACCAATAAAATTAATTAAAGATGATGTTTTAAATTTTGAATTCACAACAAGCTCAGCAACTACTATTAATCCACCAATTTGA", "aa": "MPTAVFKDENNKEYLIPALSVKNNVGYFNTKKLPLNHKYELNRIVNNNELNDVLVPNNELMLEHKVSVNKPTKAKVNFIDNKKVYEVDLGVQLKNTPLELTLEDLNHQTYKINAKTDEKGRAVFDISSLGDNNLYEVIGIKKTNEVDVVNLKQIPYHNRSINNLNSNALNTPYQYTKNGDINLIAKVAPYYVNQQVYGIFKDQNNQEHQILAKVKKDGTIAFDTGALNNNSNYSLDKIVSVSNPQNVLVSNFDLTSKQKQLIKKPAANASVDSTKKTQILENLNNDLINQKLVATFVDNNDKEYKVVANVDQNNKIIFDSNDLPKGYIYHLAKVENNDLNKVINLNDFELKDKIIDKRDLNLLDSHPDFTYDNDGNLEIHTQLANDLNDDLKQKALNNANVKGIVVDQDGIEHEIDVSIDANGKVIIPTKNLANNDPTKPNIYTLKKVVLKQNNQPNIDLISEEQLSGDNHISFKKPTITAKTKENDDYEISFSNPSLANKKIKLTFKTDDNNTNTKTVEASIGLDGKAIFKTSDDAIFAPDHKYTLTKIEADNKKVANIDEISPLDRIVNKQKNGNVNADNKHEFKIPDQKNKDLTAVYKDKNNNEIHVPIKTDDKGKVIVNPNNNLFDPNKIYDFDKIVDLNEYPNKTILDRNSINKDVSAINDGVDQARKLVLKAPAVSNVTVNAINFQVNLFDLNKLSYNRQFALTIAKVNDLNDTQKYIATYDPKTNNYKLNFDFTHLDANTKYKVVDVELLELNNKEKPIKLIKDDVLNFEFTTSSATTINPPI", "aa_hexdigest": "4fbeaaf74b3da033e7f967a68dcfb29c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 60, "aa_cov": 0.07468354430379746, "hmm_cov": 0.9833333333333333, "evalue": 6.894430674551196e-77, "score": 249.40455627441406, "start": 189, "stop": 247 } ], "seq_stats": { "molecular_weight": 89395.4051000004, "isoelectric_point": 6.128630256652832 }, "id": "GPKLONFDEH_995", "locus": "GCA000006625_00974" }, { "type": "cds", "sequence": "contig_1", "start": 573496, "stop": 574632, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "ATGAGTATTGATGAAGATCAAAAAACAATTAAAATTAAATTATTAAATTTAGATAATCATATTAATTATGAATTTGTAAGTTTTATCTTAGAAGATGTTGATGGTAATTACCATAGTGATAATTTTAATGTAAATGTTTATATAGGAGATTTTTTTGATTCTCATCCATTTCACTTGGATCTTTCATTTCTTAAATTTAAAACAAATGAGTTCAAAAAAGCTGTTTTTTCATTCACAAATACAAAATCAACTTCAACCGATCTAAATGTTGATTTACAAGACAATCAATATGCTGATAGTATTGAGCATAAAATAAAATATACTTTAGAAGATGAAAATCATAATAAATATAAGTTTGTTAAAAACTTATCAAAATCAGAAACTACTCAACTTAATGCAACAAAAAACATTATTTTACATCTTTTTGCTTTAGATAAAAATCAAACATTTAAACTTATTTCTTTTGAAATAGATGATGACAATTATTTAACTAATAATATTACAGTTAAAACACTTAATGAATCATCTCCACATTTATTATTAAATTTAGAATTTAATGATATTAAAAATACAAATAGTACTTTAAAATTAACACTAAACCAAGCAGTTATAAATATGCCAAAAAGAGTTGATGTTATTTTAAAAAATAAAAAAACAAACAAGCTGTTGTATTTATTTAAAACAGATTTTTATTTTGATAAAAAAACACACCAAATCTCAACTAAATTAGATAACTTAAACATTAATACTGATTATGAAATTAGTGAACTTTTTATTGATAAGCAACAGATTAATTTAAAAAATATCAATAATAAGACTTTTAAAACTTTAGATTACGGCTTTGATCAATTTTACTTAGATAAAATTGATCATGAAATTAACCAACAACAAAAAAACATTAAATTCAAGTTGCATTTTAGCGATTTCATCTTTAAAGATAAAAACGCCCATAAAATGTCATTAGTGTTAGAAGATGAAAACAACCAACAAGAATGTATTGATTTAAATGCTCATAACCAATTAAAAATCGACTATGAGCACCAAACAATTGAATTTGAATTAAAATATTTAAAGCCTGATATTAAATATCGAATTAATTCAATTCAATTGAATGATAAAAATTTAGATTTAACTTGA", "aa": "MSIDEDQKTIKIKLLNLDNHINYEFVSFILEDVDGNYHSDNFNVNVYIGDFFDSHPFHLDLSFLKFKTNEFKKAVFSFTNTKSTSTDLNVDLQDNQYADSIEHKIKYTLEDENHNKYKFVKNLSKSETTQLNATKNIILHLFALDKNQTFKLISFEIDDDNYLTNNITVKTLNESSPHLLLNLEFNDIKNTNSTLKLTLNQAVINMPKRVDVILKNKKTNKLLYLFKTDFYFDKKTHQISTKLDNLNINTDYEISELFIDKQQINLKNINNKTFKTLDYGFDQFYLDKIDHEINQQQKNIKFKLHFSDFIFKDKNAHKMSLVLEDENNQQECIDLNAHNQLKIDYEHQTIEFELKYLKPDIKYRINSIQLNDKNLDLT", "aa_hexdigest": "ef3ab30f2394b3220d39239f1defac22", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 80, "aa_cov": 0.21164021164021163, "hmm_cov": 0.7, "evalue": 5.527584994524943e-09, "score": 31.91581916809082, "start": 298, "stop": 377 } ], "seq_stats": { "molecular_weight": 44808.959300000024, "isoelectric_point": 5.788733100891113 }, "id": "GPKLONFDEH_996", "locus": "GCA000006625_00975" }, { "type": "cds", "sequence": "contig_1", "start": 574876, "stop": 576408, "strand": "-", "frame": 1, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X823", "UniRef:UniRef90_A0AAC9X823" ], "nt": "ATGAAATTAATTAAGAAAAAGTTATTATTTTTATTTACAGCTGCTTTAGTTCCTATTGTTGCTACAGCACTTGCATCTTGTAGCAATAAATCAAAGTTAAATGTTAATGTTTTAATGAAAGATATAATTCATACCAAGAGTGACCATTATTATGTTGTTTATGAAGTTAATGAACTAAATACTTCGCAAGGTATTTATAAGAATTTAACTTTAAACACCAATCTTTTTAATCCATTGTTAAATAAAAAAGTTGACCGTTTAGAAAATATTCATCCTTTGATTATTAATAACAAAATTTATTTAAGACTAGCTCGTAAACCAAAAATTAATGATCATTTTATCACTAATTTTTCAAATGATGCATTTCCTGCACTACATTTAATTTTTGATCATAATTTAAATTTTATCGAACAAGATATTAATGAAGATTTAAATTTACAACCATCACTTCCTAATACACCAAAGATTGATCATTTAGAAATTAATAATATTTCGTTTACAAATATTAAACAAAACCAAGTTGATATAAAGATTAAGTTAAAAACAGTTTCTTTTAAAGACGATGCTATTCATAATTTTAGTTTAAAAATTATCAAAAAAACTAATCACCAAATCATTACTAGTGATGCTGTTTTCAATAAGCAAAAAAATGAAATTGATGCTACCGTTTATAATTTAACATCTGATTGTGAATATTTATTAGATAAACTTAATTTTAATAATCAAAAAATTCAATTAACTTCAATTCCAAACCACACTTTTAAAACCCTTGATGATAGTGATAATCTTTTAAAAAATCTAGAAATTACAAAGATTAATGATAATGGTGCTAATTTAACTTTAAATTTTAAAGAGTTAAAAATAAAAGATGATAGTGATGAAGCTTTAAATAATATCCACATTAGTTTAGCATATAATAGAGTTTATAAAAAAGGTCAATTAGAGCTTAATAAGAAGCATTTTTTTATTGATAAATTAAACAAGAGAATTATTGTTTATGTTGATGAATTAGAAAGAGGAACTCATTATGATATTAAACAAATTAAGATTAATGATCAAGTTTTAAATTTAAATAATCAAACACATGATTTTACAACAAAAGGCGATTATGCTAAAGTTGAAGCTATTGATTATGAAACAGCTGTTGATAAAATTACAAAAGCAGTTACAATTAAAGTAAAATTATCTGAAGATCTTGCTTTCTACAATCCTAATATTCATAATTTTAAATTTTTAATTAGAAAAGAAAATGATCAAAACCCAATTGAATTCTCAGAAGTTAAATACGATGAACAAGAACGTTTATTAACTGCTGTAATTAATGGTTTAGATGTTGACAAAACTTATGAAATTGATAATATTTTACTAAACGGTAAACCAGTATCTTTCCCTAATGATGATGACTATGCAGGTGGATATTATGGACCTATTAACAAAACATTTAAAATACGTCCTGATCGATCAAGTATTGAAATTTTTAAAGATAAATCATTCTATAATTCTTACTATAGTGCTTTTAATAGTTGGTTTTGA", "aa": "MKLIKKKLLFLFTAALVPIVATALASCSNKSKLNVNVLMKDIIHTKSDHYYVVYEVNELNTSQGIYKNLTLNTNLFNPLLNKKVDRLENIHPLIINNKIYLRLARKPKINDHFITNFSNDAFPALHLIFDHNLNFIEQDINEDLNLQPSLPNTPKIDHLEINNISFTNIKQNQVDIKIKLKTVSFKDDAIHNFSLKIIKKTNHQIITSDAVFNKQKNEIDATVYNLTSDCEYLLDKLNFNNQKIQLTSIPNHTFKTLDDSDNLLKNLEITKINDNGANLTLNFKELKIKDDSDEALNNIHISLAYNRVYKKGQLELNKKHFFIDKLNKRIIVYVDELERGTHYDIKQIKINDQVLNLNNQTHDFTTKGDYAKVEAIDYETAVDKITKAVTIKVKLSEDLAFYNPNIHNFKFLIRKENDQNPIEFSEVKYDEQERLLTAVINGLDVDKTYEIDNILLNGKPVSFPNDDDYAGGYYGPINKTFKIRPDRSSIEIFKDKSFYNSYYSAFNSWF", "aa_hexdigest": "77e57e0665c90af42b94a41a63453e9c", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 574573, "stop": 576408, "observations": { "mutation": [ 574674, 574878 ], "stop": [ 574674, 574878 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X823", "query_cov": 1.0, "subject_cov": 0.46195652173913043, "identity": 0.996, "score": 993.0, "evalue": 0.0, "gene_start": 1, "gene_end": 510, "reference_start": 1, "reference_end": 510, "reference_sequence": "MKLIKKKLLFLFTAALVPIVATALASCSNKSKLNVNVLMKDIIHTKSDHYYVVYEVNELNTSQGIYKNLTLNTNLFNPLLNKKVDRLENIHPLIINNKIYLRLARKPKINDHFITNFSNDAFPALHLIFDHNLNFIEQDINEDLNLQASLPNTPKIDHLEINNISFTNIKQNQVDIKIKLKTVSFKDDAIHNFSLKIIKKTNHQIITSDAVFNKQKNEIDATVYNLTSDCEYLLDKLNFNNQKIQLTSIPNHTFKTLDDSDNLLKNLEITKINDNGANLTLNFKELKIKDDSDEALNNIHISLAYNRVYKKGQLELNKKHFFIDKLNKRIIVYVDELERGTHYDIKQIKINDQVLNLNNQTHDFTTKGDYAKVEAIDYETAVDKITKAVTIKVKLSEDLAFYNPNIHNFKFLIRKENDQNPIEFSEVKYDEQERLLTAVINGLDVDKTYEIDNILLNGKPVSFPNDDDYAGGYYGPINKTFKIRPDRSSIEIFKDKSFYNSYYSVFNSWFWYLSVSKKDDNERSKEVKKDYKEHLFISDITKNSAIINIKCEKLLLRKENSLKPKIKLRYHPLNNPNEWAYKEFSFEDKNCFMSIDEDQKTIKIKLLNLDNHINYEFVSFILEDVDGNYHSDNFNVNVYIGDFFDSHPFHLDLSFLKFKTNEFKKAVFSFTNTKSTSTDLNVDLQDNQYADSIEHKIKYTLEDENHNKYKFVKNLSKSETTQLNATKNIILHLFALDKNQTFKLISFEIDDDNYLTNNITVKTLNESSPHLLLNLEFNDIKNTNSTLKLTLNQAVINMPKRVDVILKNKKTNKLLYLFKTDFYFDKKTHQILTKLDNLNINTDYEISELIIDKQQINLKNINNKTFKTLDEGFDQFYLDKIDHEINQQQKNIKLKLHFSDFIFKDKNAHKMSLVLEDENNQQESIDLNTDNQLKIDYEHQTIEFELKNLKPDIQYRINSIKLNNKNLDLTWVDNQRYFNFPGVLNPNLKWKFDNIHYESVSESNVKINITFDQDIFLDNLPQDKDCNIELNYVYNTNGGDTLEQKIIAKIVHKNHVEITLTKQNCFWLDDLVYNYNHNSASDFERLLKSIKINGHKITKVNEGN" }, "paralog": false, "identity": 0.9934533551554828, "score": 1213.0, "evalue": 0.0, "description": "Internal stop codon at 574674, 574878. Nonsense mutation around 574674, 574878.", "psc": { "uniref90_id": "UniRef90_A0AAC9X823", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X823", "UniRef:UniRef50_A0AAC9X823" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X823" ], "product": "DUF1410 domain-containing protein" } }, "id": "GPKLONFDEH_997", "locus": "GCA000006625_00976" }, { "type": "cds", "sequence": "contig_1", "start": 576733, "stop": 577842, "strand": "+", "frame": 1, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_Q9PQ06", "UniRef:UniRef90_Q9PQ06" ], "nt": "ATGAATTTAAAACAAATATTTAGTGGCAAAATTCGCTTAGACGTTAGCCCACACCAACTAAAATATATCTTTGATAAAAATGAATATGTTTATTTACTTGATGATGATCACAACAATCACGTTGATTTAATCATTAAATTTTTAATTAACAAAAAAATTATTAAAAAATTAGAAGATATCACTTATTTTGGAATTCGAGCTGTTTATGTTAAAAATTTTTATGGAGCAAGTGCTTTTTTAAATAATGAAATTTATAATGATCTTAACCAACACACAGATTTATGTCCTATTCACAACCAAAATACTATCATGTTAATTAATGACATTAAGCATTTAAACACCTCTGCAAAAATTATTATTATTTTTGATAATTGGTTTCATCGTAGTATTAATAAAAATAAATACACTATTGCTATTGATCAAACAATAGCAATAAAACATAAAATTCGTAAATATGGTTTTCATGGAATTTCTTTTAGTTATAGTAATAAAATAATGCAAAATTATTTAGGAAAACAAGATGTTAATTTAATTATTTTACACTTAGGTTCTGGATCGTCAGTTTGTGCAATTAAAAATGGAAAATCATTTGATACCTCAATGTCTTACTCTCCTCTTTCAGGAATTATTATGAATACAAGAAGTGGTGATATAGACCCTAGTGTTTTACTTACTTTAAAAAAAGTAAAAAACCAATCACTGAAGCAATTGATCTTATTTTTAAATCAGCAATCAGGTCTTTATGGATTAACAAAACATCATAGTATTAGTGAAGTTGCTAATAATTTGCACAATAAAAATAATGAACTTGCCCTTGAGATGTACACTGATAGCATTGTTGATTATTTTATTAAGTATTTAAACCATTTACAAAAGATTGATGCAATTGTATTTTCGGGAGGAATTAGTGAATTTGAGCATCTTGTTTTAGAAAAAATTATTAGTAAAATTCATCTAATAAACCTTGAAATTAACGATCATTTAATACTAACAAACACGTTAAATAAAATTTCAACATCTAAATCACAAATTCCTATATATTTATGTTTAGTGAATGAAGAGCACGAAATTGCTTATGAAATGCTAAATTTAATTAATAAGACAAAATAA", "aa": "MNLKQIFSGKIRLDVSPHQLKYIFDKNEYVYLLDDDHNNHVDLIIKFLINKKIIKKLEDITYFGIRAVYVKNFYGASAFLNNEIYNDLNQHTDLCPIHNQNTIMLINDIKHLNTSAKIIIIFDNWFHRSINKNKYTIAIDQTIAIKHKIRKYGFHGISFSYSNKIMQNYLGKQDVNLIILHLGSGSSVCAIKNGKSFDTSMSYSPLSGIIMNTRSGDIDPSVLLTLKKVKNQSLKQLILFLNQQSGLYGLTKHHSISEVANNLHNKNNELALEMYTDSIVDYFIKYLNHLQKIDAIVFSGGISEFEHLVLEKIISKIHLINLEINDHLILTNTLNKISTSKSQIPIYLCLVNEEHEIAYEMLNLINKTK", "aa_hexdigest": "54d8ae14ddcbbb03fddbd9065c5cd6c6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PQ06", "query_cov": 1.0, "subject_cov": 0.9534883720930233, "identity": 0.978, "score": 663.0, "evalue": 3.48e-239, "valid": true, "gene": "AckA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_Q9PQ06", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925" }, "pscc": { "uniref50_id": "UniRef50_Q9PQ06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ06" ], "product": "Acetate kinase" }, "genes": [ "ackA" ], "id": "GPKLONFDEH_998", "locus": "GCA000006625_00977" }, { "type": "cds", "sequence": "contig_1", "start": 577846, "stop": 578838, "strand": "-", "frame": 1, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Z3", "UniRef:UniRef90_A0AAC9X6Z3" ], "nt": "TTGAGTGTTAAGATTAAATTATATTTTGAAAATAACGTTAATTATTTAAAAAATAAATATTTAAAATTAGTATACATGGATAATGCAAATAACATCATTGTATCAAAACCTGTTTTATTTGATCCCACAAAAAAAGAGTACGAATTTAGTTTCGATAAAGTTTTATCAAACCGAAAATACACTTTTTCTAAACTAGTTTTTGATGATCTACAAGATTTTAGTGAAAAACAAGCTTTAGAAGTAAATACTCAAGGATTAAATGGTAGTTTTAATACACCTCAAGCAAAAATTAAAATCAAAAAGATTTCTTATAGTTCAACCGATAAATCCGCTAAATTAGAGTTTCATTTAGAAGATGATTTTGATTTAGTTGATGGTGATAAAGTTAGTGTCAAATATAGACTAAAACCATCATCTAATAGTATTGATACTACTAATAATACTGTTGAGGGTTTGGTTCATAACCACATATTGAATGTAACAATTAATGATTTAGAATCAAATAAATCATATGAAGTTATTGACTTTGTTATTAATGATTATAAAAATAAACAATTATTAAACAAACCTGAATTTTTAAAAAAAAATACAAATCATCATAACCATCCTTTTAATATCCCGCTTGATTTTGATACTAAAAATAGTGATTATAAAACTAAATTTAATGTAAATGAAATTAGTAAATCTTATAATAAAGAAACTAAATTAACAACAATCAAATTAAAATTTGATAATATTCCCACAAATCCCACTTCATCTAATAATTTTACATTTACAATTAAAAAACGAACAGGATACACTACTAATCTGATAAGCCCAAAAACTTTGGAATATGATGTAAATAAGAAAACTTTAATAGCAAAATTTGAAAATATTGAGGACCAAATTGTTTATGATTTTGTTGATGTTAAAGTAAATAATCAAAATATTGAATTTAACAAATCATTAGATAAGTATTTTTCTATTATTCATAGTGCTGACTATCATGAATAA", "aa": "MSVKIKLYFENNVNYLKNKYLKLVYMDNANNIIVSKPVLFDPTKKEYEFSFDKVLSNRKYTFSKLVFDDLQDFSEKQALEVNTQGLNGSFNTPQAKIKIKKISYSSTDKSAKLEFHLEDDFDLVDGDKVSVKYRLKPSSNSIDTTNNTVEGLVHNHILNVTINDLESNKSYEVIDFVINDYKNKQLLNKPEFLKKNTNHHNHPFNIPLDFDTKNSDYKTKFNVNEISKSYNKETKLTTIKLKFDNIPTNPTSSNNFTFTIKKRTGYTTNLISPKTLEYDVNKKTLIAKFENIEDQIVYDFVDVKVNNQNIEFNKSLDKYFSIIHSADYHE", "aa_hexdigest": "84d01fba2ecbeb1c093d73a3e7e63a14", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 577846, "stop": 579138, "observations": { "mutation": [ 578856 ], "stop": [ 578856 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6Z3", "query_cov": 1.006060606060606, "subject_cov": 0.4695898161244696, "identity": 0.958, "score": 611.0, "evalue": 2e-214, "gene_start": 1, "gene_end": 330, "reference_start": 376, "reference_end": 707, "reference_sequence": "MIIKKHLNKIITILTCSTLIIGLSCLIGACTKSKSKVNDKQVLFKIKKFKTTTNSIELFLEGSAVLASHSYSVVLKEKASGVLKTINNLKLVKDNDLNKLIINELKPNMGYELVDLLINNQKQLITKLDFITSTLAQENVDQFSIKKIDWKNITNNSADLNLEFTKYFLANENQNIFEISLLNLQTQQITKFKFNYQPNSPSITFNFTQLENNAKYQIHRIVLNNKELVFSNQNLVLSNTNKQNSNPIIKDLNITKIEKEIYDTSAKLIFYFKDNKLGDLNNQKFVLRLVSAHQPSNTFSFENYVYNNSKKTLSFNLTGLQTSTKYSLNSLTLNEQPISLANLDLNILTSTKLTTVQRLENLEHESSDFWFNDENLSVKIKLYFENNVNYLKNKYLKLVYMDNANNIIVSKPVLFDPTKKEYEFSFDKVLSNRKYTFFKLVFDDLQDFSEKQALEVNTQGLNNSFNTPQAKIKIKKISYSSTDKSAKLEFHLEDDFDLVDGDKVSVKYRLKPSSNSIDTTNNTIEGFVHNHILNVTINNLESNKSYEVIDFVINDYKNKQLLKEPEFLKDNTNHHNHPFNIPLDFDTKNSDYKTKFNVNEISKSYNKETKLTTIKLKFDNIPTNPTSSNNFTFTFTIKKRTGYKTNPISPKTLEYDVNKKTLIAKFENIEDQIVYEFVDVKVNNQNIEFNKSLDKYFSIIHSADYHE" }, "paralog": false, "identity": 0.9629629629629629, "score": 826.0, "evalue": 1.32e-301, "description": "Internal stop codon at 578856. Nonsense mutation around 578856.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6Z3", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6Z3", "UniRef:UniRef50_A0AAC9X6Z3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Z3" ], "product": "DUF1410 domain-containing protein" } }, "id": "GPKLONFDEH_999", "locus": "GCA000006625_00978" }, { "type": "cds", "sequence": "contig_1", "start": 578854, "stop": 579162, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGATTTTTTATTTTAAAGATAATAAGTTAGGTGATTTAAATAATCAAAAATTTGTTTTAAAATTAGTTTCAGCACACCAACCATCAAATACTTTTAGTTTTGAAAATTATGTTTACAATAACTCTAAAAAGACCTTATCATTTAATTTAACGGGTTTACAAACTAGTACAAAATACTCATTAAATTCATTAACTCTTAATGGACAGCCAATATCATTAGCTAATTTAGATTTAAATATTTTAACCTCTACAAAATTAACTACTGTTCAAAGATTAGAGAACTTAGAACATGAAAGCAGTGATTTTTGA", "aa": "MIFYFKDNKLGDLNNQKFVLKLVSAHQPSNTFSFENYVYNNSKKTLSFNLTGLQTSTKYSLNSLTLNGQPISLANLDLNILTSTKLTTVQRLENLEHESSDF", "aa_hexdigest": "a7d6ad5b151b5c5a58cd2d29bb644703", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 65, "aa_cov": 0.6372549019607843, "hmm_cov": 0.7538461538461538, "evalue": 1.2740714007613586e-10, "score": 37.1600341796875, "start": 9, "stop": 73 } ], "seq_stats": { "molecular_weight": 11611.931499999986, "isoelectric_point": 7.985263633728028 }, "id": "GPKLONFDEH_1000", "locus": "GCA000006625_00979" }, { "type": "cds", "sequence": "contig_1", "start": 579514, "stop": 579963, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACTAAAAAACATTTAACTAAAATAATTACAATTTTAACTTGTAGTACTTTAATTATTGGTTTATCTTGCTTAATTGGTGCATGCACTAAATCAAAAAGTAAGGTTGATGATAAACAAGTGCTTTTTAAAATAAAAAAATTTAAAACAACTACTAATAGTATTGAGTTATTTTTAGAAGGTAGTGCAGTTTTAGCATCACATTCATACAGTGTTGTTTTAAAAGAAAAAGCAACTGGTGATTTAAAAACAATTAATAATTTAAAATTAGTTAAGGATAATGATCTTAATAAATTAATTATTAATGAATTAAAACCAAATATGGGTTATGAATTAGTAGATTTATTAATTAATAATCAAAAACAGCTAATAACTAAACTAGATTTTATAACAAGTACATTAACACAAGAAAATGTAGATCAATTTAGTATAAAAAAAATTGATTGA", "aa": "MITKKHLTKIITILTCSTLIIGLSCLIGACTKSKSKVDDKQVLFKIKKFKTTTNSIELFLEGSAVLASHSYSVVLKEKATGDLKTINNLKLVKDNDLNKLIINELKPNMGYELVDLLINNQKQLITKLDFITSTLTQENVDQFSIKKID", "aa_hexdigest": "665fd401df4a6a8f5f292f724b4595fe", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16685.54969999999, "isoelectric_point": 9.30170803070068 }, "id": "GPKLONFDEH_1001", "locus": "GCA000006625_00980" }, { "type": "cds", "sequence": "contig_1", "start": 580148, "stop": 580291, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATATTGTTGATAATTGGGATGAAATAGCAAAATCAAATGATGTATACATTAAAAAAGCTACTTCTATTATCTCAGATCATACTTTAGTTGCTGTTAATATTAAAATCGATGAAAATGATATTATTGATGAAAATTAA", "aa": "MQNIVDNWDEIAKSNDVYIKKATSIISDHTLVAVNIKIDENDIIDEN", "aa_hexdigest": "61a0e83a2488d8e34be2cb22b3dcfabe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5358.8949, "isoelectric_point": 4.197752952575684 }, "id": "GPKLONFDEH_1002", "locus": "GCA000006625_00981" }, { "type": "cds", "sequence": "contig_1", "start": 580379, "stop": 581014, "strand": "-", "frame": 3, "gene": null, "product": "Membrane nuclease A-hypothetical", "db_xrefs": [ "COG:COG2374", "COG:R", "SO:0001217", "UniRef:UniRef50_Q9PQ04", "UniRef:UniRef90_Q9PQ04" ], "nt": "GTGCTATCAAAAAACTTATTTTCAAAAGGCAATGAATCACAAACTGAACGAATTGGCTTCTTTTATAAAGCTAATATAATAAAACCAGTTCAAGAATATATTTACAAAAACAATGATAATAAAGATATTTTGTTTGAACAATATTTTAAGAACTGGAATGATAATACTAAAATTACTTATTCACGTCCGCCTTATGCCTATGAATTTCAAACTTTAGATAACAAATTTAATTTTACAATTGTCGCTAGTCATTTAGATTCACCAGGAGCAAACGAACATAAAACTAGCAAACATGATCATACTTATGGTAATAAAAATTTAAAAATTAATATTAAACAAGGCTCAAAGGAATTAAATGAGGCATATCAATTAGGAAATGTGATGGACTATATTGATAGATTAGATGGAAATAATGATGATTTAATTTTTGTTGGTGATACTAATATTAAAACAAAAAACGAATTAGGAGCCTTTGCTTCACTTTATAAACGTAATTATTTATCCAATTTTAAGGATGATAATGAAAATAACAAAACTACATTAGCAAAAACTTATTTAAAATACGCACAACACTATGACAAAATTTTTACTTCACCATCAATTAAAGTTTTAAATACTTATAAGTATGATTTATGA", "aa": "MLSKNLFSKGNESQTERIGFFYKANIIKPVQEYIYKNNDNKDILFEQYFKNWNDNTKITYSRPPYAYEFQTLDNKFNFTIVASHLDSPGANEHKTSKHDHTYGNKNLKINIKQGSKELNEAYQLGNVMDYIDRLDGNNDDLIFVGDTNIKTKNELGAFASLYKRNYLSNFKDDNENNKTTLAKTYLKYAQHYDKIFTSPSIKVLNTYKYDL", "aa_hexdigest": "1d6dd664ad380514556269f7ed3c8f3a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 580148, "stop": 581287, "observations": { "mutation": [ 580342, 580381, 581023, 581221 ], "stop": [ 580342, 580381, 581023, 581221 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PQ04", "query_cov": 1.0, "subject_cov": 0.47845804988662133, "identity": 0.991, "score": 418.0, "evalue": 1.75e-144, "gene_start": 1, "gene_end": 211, "reference_start": 154, "reference_end": 364, "reference_sequence": "MKNNKSRKWVITISLTLLIVLITSAVIGVVFYLKNKKKNDTSVNNDSYIIQNNNNNNNNNNNSLKEQPNSTRSFLNQKTICVGHWNVLNQGGINQLKNEALAKVILKSNVDVIALTEINSAKNNEHDDLPMRAILKELNLHKNQFDQTANWNYVLSKNLFSKGNESQTERIGFFYKANIIKPVQEYIYKNNDNKDILFEQYFKNSNDNTKITYSRPPYAYEFQTLDNKFNFTIVASHLDSPGANEHKTSKHDHTYGNKNLKINIKQGSKELNEAYQLGNVMDYIDRLDGNNDDLIFVGDTNIKTKNELGAFASLYKRNYLSNFKDDNENNKTTLAKTYLKYAQHYDKIFTSPSIKVLNTYKYDLWSVSLTDTLLDKNWKLAFLKTYFRSLSNQQMQNIVDNWDEIAKSNDVYIKKATSIISDHTLVAVNIKIDENDIIDEN" }, "paralog": false, "identity": 0.9841688654353562, "score": 744.0, "evalue": 2.58e-275, "description": "Internal stop codon at 580342, 580381, 581023, 581221. Nonsense mutation around 580342, 580381, 581023, 581221.", "psc": { "uniref90_id": "UniRef90_Q9PQ04", "product": "Membrane nuclease A-hypothetical", "uniref50_id": "UniRef50_Q9PQ04", "cog_id": "COG2374", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PQ04", "UniRef:UniRef50_Q9PQ04", "COG:COG2374", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ04", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ04" ], "product": "Membrane nuclease A-hypothetical" } }, "id": "GPKLONFDEH_1003", "locus": "GCA000006625_00982" }, { "type": "cds", "sequence": "contig_1", "start": 581021, "stop": 581164, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTCTAAAAAGTAATGTTGATGTAATTGCTTTAACAGAAATTAATAGTGCAAAAAATAATGAACACGATGATTTACCAATGCGTGCCATCTTAAAAGAATTAAATTTACACAAAAATCAATTTGATCAAACAGCTAATTGA", "aa": "MILKSNVDVIALTEINSAKNNEHDDLPMRAILKELNLHKNQFDQTAN", "aa_hexdigest": "23695ef5618344d21c808dcaf0020923", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5361.030000000001, "isoelectric_point": 5.425304603576659 }, "id": "GPKLONFDEH_1004", "locus": "GCA000006625_00983" }, { "type": "cds", "sequence": "contig_1", "start": 581219, "stop": 581404, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAATCATTTTAATAGCAAGTGCAGTTATTGGCGTTGTTTTTTATTTAAAAAATAAAAAAAAGAATGATACAAGTGTAAATAATGATAGTTATATAATTCAAAATAATAATAATTCTTTAAAAGAACAACCTAACTCAACAAGATCATTTTTAAACCAAAAAACAATTTGTGTAGGTCATTGA", "aa": "MLIILIASAVIGVVFYLKNKKKNDTSVNNDSYIIQNNNNSLKEQPNSTRSFLNQKTICVGH", "aa_hexdigest": "a56d22efd559ac0d1495b6126dfbdd78", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6852.8047000000015, "isoelectric_point": 9.580920600891112 }, "id": "GPKLONFDEH_1005", "locus": "GCA000006625_00984" }, { "type": "cds", "sequence": "contig_1", "start": 581973, "stop": 582353, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAGATAGTTTAAGTTTACATCAAAGCTTAAATCCATCAGATTATGCTTTAAATTTTTATAATCTTGATACTAATATTATTAACCGAAAATTTACACTTAGTCCCCAAACTGTTTTATCTTCAAAAGTTAATAATTCAAATGTAATTGAATTAATTTATGATTTAGATAATGATGAAAATATTTATGGAGGTTTAGAACCTGAGAGCACTTATCAAATTAGTTCGTTAATGATAAATGCTAAACCAGTGATTATTAATACTTCTATAACAATGGTTCAAACTAACGCTTCAGCTTCTAAGGTAGAAAAAATTGTTGCTAGTTATGATTCAGTAACCAAAAAAACAAAAATATCATTGCAGTTTTCTAATAATTGA", "aa": "MKLDSLSLHQSLNPSDYALNFYNLDTNIINRKFTLSPQTVLSSKVNNSNVIELIYDLDNDENIYGGLEPESTYQISSLMINAKPVIINTSITMVQTNASASKVEKIVASYDSVTKKTKISLQFSNN", "aa_hexdigest": "4c51911bd9aa19da52536573c52f35f2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14039.673799999984, "isoelectric_point": 5.229266929626463 }, "id": "GPKLONFDEH_1006", "locus": "GCA000006625_00985" }, { "type": "cds", "sequence": "contig_1", "start": 582390, "stop": 583460, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAAAATGATGGAATTCCACAACAATTACAACTTTCATATGATCATTTTATTAACCCATATAATTTTAAAGATAATATGTTAAGTGCAGATATTGAACTTATATCAACTAAAAATAATAAAACTATTATTTTAAAATCATTAAAGTTTAATCACGATAAAAACAATATTGTTTTTGATTTAGTCAATAATTTAGAATTACAAACTCTTATTTTAAAAACTCCTTTTTTTAAAATTAAATCATTAACAATAATGAATGATAAGCAAACTTTGCCATTAAGTATTAGAGAAAAACAGCTTGATTTAACTAATAAACATCTTTCTAAAATCATTAAATTTACATTTGATGATACTGATCCACAAAATATGCTTATGAAATTTCAATTAGATAGTGATTCGCCAATAGTGCAAAATACTAAAAAAGTTTTATTAACAATTAAAAAAATAAATACAGATCAGCAAGAAGATATAAAGACCTTTAGTTTTGATATTTTAGAAAACAAAATAACAACAGGTTCTTTTTATGATGTAAAAAATAAGTATTTTCTATTAAATCTAAATAAAACTTTGAATGCTAAATCGATAGGTCAAATTCTTGAAAATGATTCTAATTATATAATTACTAAATTAACTGTGGTTAATAATGAAGCATCAATCAATGAAATACCAATTGAAATTGATGATTTACAAAATAAACAATTTACGTTAAAATTTGCTGAATACCAATCAAATGTAATTAAATTTAATGCTAAGAATGATCCTGAATTACATCTTAATATCAAACATTTTAAACAACTTAATCTTACAAAAATTAATAAAATTAAATTGACTTTAAAAGACGGTAAGCAAATTATTTTTAATCGTTTAAATAATGAATTTAGTTTCCAACAAGCAGGATTTAAATTAAAAATTAACCAAAACACATTTCCAAATTATTTAAAACAACATCAATATGTAATTAAAAGTATTAGTTTAATAAACGATGATCAGTCTGTGATTGAGTTATTATCAAAACACAATCATGAAGCTATTTTTGAGATTCCTATTACAGCATCTCTTGATACTTGA", "aa": "MIKNDGIPQQLQLSYDHFINPYNFKDNMLSADIELISTKNNKTIILKSLKFNHDKNNIVFDLVNNLELQTLILKTPFFKIKSLTIMNDKQTLPLSIREKQLDLTNKHLSKIIKFTFDDTDPQNMLMKFQLDSDSPIVQNTKKVLLTIKKINTDQQEDIKTFSFDILENKITTGSFYDVKNKYFLLNLNKTLNAKSIGQILENDSNYIITKLTVVNNEASINEIPIEIDDLQNKQFTLKFAEYQSNVIKFNAKNDPELHLNIKHFKQLNLTKINKIKLTLKDGKQIIFNRLNNEFSFQQAGFKLKINQNTFPNYLKQHQYVIKSISLINDDQSVIELLSKHNHEAIFEIPITASLDT", "aa_hexdigest": "5ffdff880481187b0f375e9de5af31ea", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41414.2008, "isoelectric_point": 8.93223972320557 }, "id": "GPKLONFDEH_1007", "locus": "GCA000006625_00986" }, { "type": "cds", "sequence": "contig_1", "start": 583488, "stop": 584150, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAATGCCAAAAGTTATTTTTTCAACAAGTATTATTAATAGTTCAAACCAAAAAATTGCTTTTTATGTTGATATAAAACCAATTATTATAAATAATCGTATTTATATTCGCTTACCCCAACGTCCTAAACCCAATGAAAAAATTATTATAAATTCAAGTCAAGAATTTGTTGGCGTTCAAGTGATTCAAACTAATAAAATGTTGACTAGATCCATTAATTTTAATTTTAATAAAGAAGATCATTTAATTAAAGTTGTTGACCAGAGTGCCCGATTTACTAACATTAAAAAAACAGAAGTTGAATTCGAAATTAAGCTTTTAAATTTAAATCTTAATGATATTAAAGATAAAAATATTGAAATTGAATTAACAAATAAAAAAACAAATAAAACCTTTAAATTGCATTTTCTAAAATATCAATTTAATCACTTAACACAACAAAATAAAAAACCAGTATTAACTATTACTGCTAATTTAAATGCTGATGCAAATAACCAATTAGATGATGGCGAATATTTTATTTCTAAATTAGTTTTAGACCAAAAAAAACTTGAAATTAACCAAAGCGTTTATAAACCAAGTGGGTTACAGTTAGATGGAAGTATTAATAATCAAAGACCAACTTATTTATTAATTCCATCTGTTTTTTCAAAATGA", "aa": "MQKMPKVIFSTSIINSSNQKIAFYVDIKPIIINNRIYIRLPQRPKPNEKIIINSSQEFVGVQVIQTNKMLTRSINFNFNKEDHLIKVVDQSARFTNIKKTEVEFEIKLLNLNLNDIKDKNIEIELTNKKTNKTFKLHFLKYQFNHLTQQNKKPVLTITANLNADANNQLDDGEYFISKLVLDQKKLEINQSVYKPSGLQLDGSINNQRPTYLLIPSVFSK", "aa_hexdigest": "b4c72483f98fbcfb41da989d79570f0f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25566.29930000002, "isoelectric_point": 9.717464637756347 }, "id": "GPKLONFDEH_1008", "locus": "GCA000006625_00987" }, { "type": "cds", "sequence": "contig_1", "start": 584559, "stop": 585080, "strand": "-", "frame": 2, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_006688428.1", "SO:0001217", "UniParc:UPI0000125CF5", "UniRef:UniRef100_Q9PQ02", "UniRef:UniRef50_Q9PQ02", "UniRef:UniRef90_Q9PQ02" ], "nt": "ATGATCAATATTGATTACATTAAATCAAAAATCAGGGATGTACCAGACTTCCCTAAAAAAGGAATCGTTTTTAAGGATATTACTCCTTTATTTTTAGAACCAAAAATTATTGAAAAGATTGTTGATGATTTTGCTGACTTTGCTAAATCATTAAATATAGATGCTATAATAGGTGCGGAGTCGCGTGGTTTTTTGTTTGCAGCACCATTATCAATCAAATTAAATAAGCCATTTATTTTGGTACGTAAACCAAATAAGTTACCAAATGATGTTTATAGTGCTGAGTACACATTAGAATATGGTAGTTCTCGTGTGGAGATGCACAAAGATGCACTAAAACCAAACCAACGCGTTTTAATTGTTGATGATTTATTAGCAACAGGAGGGACTGTTGCTGCGATTGAAAATCTTGTTCGTCAAGCTAAAGGAATTGTAGCAGGGAGTGTTTATTTAATTCGTTTAGGATTTCTAAAAGGTGAAGAAAAACTAAGTGGAAAAGTTCATGCTTTAATTAATTATTAA", "aa": "MINIDYIKSKIRDVPDFPKKGIVFKDITPLFLEPKIIEKIVDDFADFAKSLNIDAIIGAESRGFLFAAPLSIKLNKPFILVRKPNKLPNDVYSAEYTLEYGSSRVEMHKDALKPNQRVLIVDDLLATGGTVAAIENLVRQAKGIVAGSVYLIRLGFLKGEEKLSGKVHALINY", "aa_hexdigest": "867fe2aff922d6c30268f4bc917ad39d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125CF5", "ncbi_nrp_id": "WP_006688428.1", "uniref100_id": "UniRef100_Q9PQ02", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125CF5", "RefSeq:WP_006688428.1", "UniRef:UniRef100_Q9PQ02" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ02", "uniref90_id": "UniRef90_Q9PQ02" }, "psc": { "uniref90_id": "UniRef90_Q9PQ02", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_Q9PQ02", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ02" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "GPKLONFDEH_1009", "locus": "GCA000006625_00988" }, { "type": "cds", "sequence": "contig_1", "start": 585101, "stop": 585208, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGGTTTATATTAGTAAAGGTTTATTAGCTAAGGATGATAATGCAGATAATGTTTATGATAAATTTGATGAACAAGAAATTATGAATATAAATAAATTTTAA", "aa": "MKMVYISKGLLAKDDNADNVYDKFDEQEIMNINKF", "aa_hexdigest": "f617e08375b5f10d19b9d2607ed9a610", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4141.6978, "isoelectric_point": 4.6018781661987305 }, "id": "GPKLONFDEH_1010", "locus": "GCA000006625_00989" }, { "type": "cds", "sequence": "contig_1", "start": 585314, "stop": 585478, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAGTTTTAGTTTACATATTTGTTTAAATTTAAAACGAACTTTGGATTTAATGAAGCATGTAACAAAAAAACAATTACAATTTCAAATTCAAGATTCTTTAATTAAATACTTTAATACTTATAATATAATATACATTGTGATATTATTTGCATTATTGTGA", "aa": "MTSFSLHICLNLKRTLDLMKHVTKKQLQFQIQDSLIKYFNTYNIIYIVILFALL", "aa_hexdigest": "02d481f37470b0ad2e2e51f0288fddc1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6476.756799999997, "isoelectric_point": 9.580985069274899 }, "id": "GPKLONFDEH_1011", "locus": "GCA000006625_00990" }, { "type": "cds", "sequence": "contig_1", "start": 585626, "stop": 586006, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAAAGGTTTATAGAATTAGTTTTTTAATTTTTATCTTTATTTTAGCTTTTATTGGCTTATTTATTTTAGGTTTTATTGTTCCAAACCATAGTTTTAGTTTTGGTTCTGTATATGAGTTAATTATGAAAAGTGATTTAAAAGACTTTAATTCTCACGAGTTCAAAACTATTTTAGATTATAAGATTAATGATGGAGTAGTATCAATGCAATTTGATGCTAAAAAACCAAATGTTTTAAGTGAAATAGATTTGATCAGTTTAAATCACCGGTTTAGTGATGCAATTGTTTATAGAAGTAGTCAATATCATTTAATTAATAATATTACAAATTCAATTGCTGCGTATTTTATTATCTTTGCTATTATTTTAATTTGA", "aa": "MNQKVYRISFLIFIFILAFIGLFILGFIVPNHSFSFGSVYELIMKSDLKDFNSHEFKTILDYKINDGVVSMQFDAKKPNVLSEIDLISLNHRFSDAIVYRSSQYHLINNITNSIAAYFIIFAIILI", "aa_hexdigest": "904e1f6a0e520f02bb8c178f1e3678af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14580.894399999985, "isoelectric_point": 6.8981733322143555 }, "id": "GPKLONFDEH_1012", "locus": "GCA000006625_00991" }, { "type": "cds", "sequence": "contig_1", "start": 586145, "stop": 586609, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCGATTTTGATTGGAATTGCAGTTTTAATCTTAATCATCGCTATTATTATTAGTCTTTTATATAAAATTCCAGGTTTAATTTATAGTTTGTTAATGGCTTTTAGTTTTGTTATTAGTTTATTATTAATAAAATCAAGTAATTTAGGATTTAGTACAGAAACTTATAGTGCATTAATTATTGGTATTTGTTGGCCATTATTAAATTTAGTTGATTTCAATAACCATATAAAACATTTAATTCAACAAAAATTTTCTTATAAAAACGCTTTAAGAATTAGTTTAAATAAAACGATTGTTAATCAAGGAATGTTTTATTTATTAATGATTTTTATCAGTTTAGTTTTTATGTATTTTGGTAAAAATAACATTAGTATTTTTGGTTTTAATTTGATTTTAATAACTTTTTCTTGTTTGTTAATATCTTATATATTATTTATTGTAATGATGTATATGTTGTGA", "aa": "MDAILIGIAVLILIIAIIISLLYKIPGLIYSLLMAFSFVISLLLIKSSNLGFSTETYSALIIGICWPLLNLVDFNNHIKHLIQQKFSYKNALRISLNKTIVNQGMFYLLMIFISLVFMYFGKNNISIFGFNLILITFSCLLISYILFIVMMYML", "aa_hexdigest": "8ca53cd62b9f61be412aab392d0a1f2b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17579.420699999995, "isoelectric_point": 9.329816246032717 }, "id": "GPKLONFDEH_1013", "locus": "GCA000006625_00992" }, { "type": "cds", "sequence": "contig_1", "start": 587321, "stop": 587485, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAAAACTAGTTTTGATCATCAAAAATTTAATTTAGATTTTTTTGCTAAAGAGTTTGATGCTAATTTTAATTTCAATCCAATGGGTAATAAAACAAGAGCTCAAGCTGTAGCGACTTTTGATAGAACAAAACAAAAACGAAAAGCTCCAGTTAATTCTTGA", "aa": "MPKTSFDHQKFNLDFFAKEFDANFNFNPMGNKTRAQAVATFDRTKQKRKAPVNS", "aa_hexdigest": "b6b49518694eecc805baceb7a0917c1c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6284.021100000005, "isoelectric_point": 10.11536350250244 }, "id": "GPKLONFDEH_1014", "locus": "GCA000006625_00993" }, { "type": "cds", "sequence": "contig_1", "start": 587558, "stop": 587929, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATTTTCAAACAGAAGCAGAGCAGTTAAATTTATTAAAAACCACAGCGAATCTAAGTCAAAAACACTTACAAGCATTGGGTGTAAATAATGTTAGTGCTAAATATGCTATTTATGAACGTATTAATAAAAATACAAAAAAACATGAAAAATTTGGGGAAATTGTTTATCAATTTTATCCTACAAATTCAAAATTAGATATCGTTAATTTTCTAACTAAAACAAAGCCATACGAAAAAATTAATTCAAAAATCCAACTTTTAAGCTTATTTACTACCGCTAACCGTTTAGAATTACAAAACATTTCTTCACTTTTTTCACCAAAACCTGAACTTTCACAAAACAGGGATTTTGATAATACAAATTGA", "aa": "MNDFQTEAEQLNLLKTTANLSQKHLQALGVNNVSAKYAIYERINKNTKKHEKFGEIVYQFYPTNSKLDIVNFLTKTKPYEKINSKIQLLSLFTTANRLELQNISSLFSPKPELSQNRDFDNTN", "aa_hexdigest": "3ab915bb99d691726bb473c6dff9df7c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14243.960399999976, "isoelectric_point": 9.30106334686279 }, "id": "GPKLONFDEH_1015", "locus": "GCA000006625_00994" }, { "type": "cds", "sequence": "contig_1", "start": 588434, "stop": 588901, "strand": "+", "frame": 2, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "EC:3.1.-.-", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872", "RefSeq:WP_006688705.1", "SO:0001217", "UniParc:UPI00000C1C90", "UniRef:UniRef100_Q9PQ00", "UniRef:UniRef50_Q9PQ00", "UniRef:UniRef90_Q9PQ00" ], "nt": "ATGCATTTTTTAATAACGAATGAAGTTAAAAAAATCTTTAATGATAAATTATATTCACAGCGTTTTAAACAAATAACCGATTTGGTCTCCACAAAATTAAATATTGATAAAAAGCGATTTTTTGAATGTCATTTTGTTGATGAAAAAACGATACAAGAAATTAATAAAAACTATCGTAATAAAGACTATATTACAGATGTTATTAGTTTTGCATTTGATGATGGCGAAATTATTACTCCTTTATTGGGAGAAATGTACATTTGCTATCAAAAGGTTGTTGATCAAGCAAGTCAATTTGCTCATTCATTCGAACGTGAACTATGCTTTTTATTTACACATGGTTTACTTCATTTATTAGGTTATGATCATATAAAAATTGAAGATGAAAAAATTATGTTTGCTTTACAAGATGAAATTTTAAATGAATTAAAAATTACAAGAAATATAAATGGTGCTAGAAATGATTAA", "aa": "MHFLITNEVKKIFNDKLYSQRFKQITDLVSTKLNIDKKRFFECHFVDEKTIQEINKNYRNKDYITDVISFAFDDGEIITPLLGEMYICYQKVVDQASQFAHSFERELCFLFTHGLLHLLGYDHIKIEDEKIMFALQDEILNELKITRNINGARND", "aa_hexdigest": "6f7d29d3f750eb2c958cc93468164e2a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1C90", "ncbi_nrp_id": "WP_006688705.1", "uniref100_id": "UniRef100_Q9PQ00", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C90", "RefSeq:WP_006688705.1", "UniRef:UniRef100_Q9PQ00" ] }, "ips": { "uniref100_id": "UniRef100_Q9PQ00", "uniref90_id": "UniRef90_Q9PQ00" }, "psc": { "uniref90_id": "UniRef90_Q9PQ00", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_Q9PQ00", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQ00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQ00" ], "product": "Endoribonuclease YbeY" }, "genes": [ "ybeY" ], "id": "GPKLONFDEH_1016", "locus": "GCA000006625_00995" }, { "type": "cds", "sequence": "contig_1", "start": 588882, "stop": 589745, "strand": "+", "frame": 3, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_Q9PPZ9", "UniRef:UniRef90_Q9PPZ9" ], "nt": "ATGGTGCTAGAAATGATTAAAAAATACGGAATCGTAGCTATTGTTGGAAAACCAAATGTTGGTAAATCAACATTAATCAATGCGATTATGAAAAAAAAAGTCTCAATCATTTCAAATAAGCCGCAAACAACACGCAATGCTGTTAAAGAGATTTATGAAGATGATGAATCAGCAATTATTTTTACTGATACGCCTGGTTTTCATGAACCATCAAATAAACTAGATTTATTTTTAAATCATGAAATTGAAATTTCATATAAAGAAGCAAATGTAATCTTATTCGTGACAACTATGGACAAGGAATTAGATGCCAATGATTTTGAAATTATTAATTTAATTAAAGAAGCAAATAAAGAAAATATTATTTTAGTCATTTCAAAAGCTGAAATGGCTAAAAATCAAGATCAAATTGATGAAAGAATCCACTTTTTAAAAAAACATATAGCATTTAAGGATGTTGTTCAAATAAGTGCTTTACATGTTATTAACATTGATAAATTAATTAATACAATCAAAAATTACCTTCACAAAGATGTGGTAACCGATTATTTTCGTCAAAAAGCCGAAAAAGAAGATAAATTTGTTATTACTGAAATTATTCGTGAACAATGCTTATTAAATTTAAACCATGAAGTTCCTCACGGTGTTGGTGTAGAAATTGATGAAAGTAAATACAACCAAGAAGCAAATCATTGGATAATTAAAGCTTCAATAATAATTGAAAAAAATTCTCATAAACCTATTATTATTGGCCAAAATGGTACGATGATTAAAAAAATTAGCATGGGGGCAAGAAAGCAGTTGCATGAAATTTATGATTGTCATATTTCATTAACAATTTTTGTTAAAGTTGAAAACAATTGA", "aa": "MVLEMIKKYGIVAIVGKPNVGKSTLINAIMKKKVSIISNKPQTTRNAVKEIYEDDESAIIFTDTPGFHEPSNKLDLFLNHEIEISYKEANVILFVTTMDKELDANDFEIINLIKEANKENIILVISKAEMAKNQDQIDERIHFLKKHIAFKDVVQISALHVINIDKLINTIKNYLHKDVVTDYFRQKAEKEDKFVITEIIREQCLLNLNHEVPHGVGVEIDESKYNQEANHWIIKASIIIEKNSHKPIIIGQNGTMIKKISMGARKQLHEIYDCHISLTIFVKVENN", "aa_hexdigest": "42b4787fc03c9e37c9fe62225b211742", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PPZ9", "query_cov": 0.9860627177700348, "subject_cov": 0.9433333333333334, "identity": 1.0, "score": 549.0, "evalue": 6.54e-197, "valid": true, "gene": "era", "product": "GTPase Era", "uniref50_id": "UniRef50_Q9PPZ9", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ9" ], "product": "GTPase Era" }, "genes": [ "era" ], "id": "GPKLONFDEH_1017", "locus": "GCA000006625_00996" }, { "type": "cds", "sequence": "contig_1", "start": 589796, "stop": 590512, "strand": "+", "frame": 2, "gene": "recO", "product": "DNA repair protein RecO", "db_xrefs": [ "COG:COG1381", "COG:L", "GO:0003677", "GO:0006281", "GO:0006310", "KEGG:K03584", "RefSeq:WP_010891799.1", "SO:0001217", "UniParc:UPI00000C1C91", "UniRef:UniRef100_A0A2C9DYJ4", "UniRef:UniRef50_A0A084F1G9", "UniRef:UniRef90_A0A2C9DYJ4" ], "nt": "ATGGCTGAAATAATAACTAGAGGATATTTAGTTGCGCGCCAGGATTATGATGTTTTTGATGAAATATTAACATTTATTAATGAATATGGTAATCGTTTTATTATGTTTGCACCTGGAACAAAAAGAATTACTTCTAAGAACGCAAGAGCTTTGTTTTTTGGTAATTTTTTAGAAATACAATTTTTTTATGCAAGAAATGATACAAAACTTTCAAAATTAAAAAAAGTTATTTCTCTAGATCAAATTGATTATAAGTACGAAAATACATATTCAATGCTAATATTAAGCGAATTAATGACTAAAGTTGTTAATTTTAATGTTGAATTCTACCAATTCTATCAGCTAATACTAGAATATATCATTTTAGAATATAATGATTATTATATAAGCTGTTTTTTATTGGTAAAATTTTTAATAATGAATGGAGTTAATTTTAATTTTCAAGCATGTGTTCATTGTAATAGCTCAAAAAATATTAAAACTTTTTCTTTGGCTGATCATGGTCTTATATGTATTAACTGTGAATCTAAAATTATTAATAAAATAGATTATGAACCAAAGGCGTTACAATTATGGCAGAAACTTTATTTTGCAACACAGATAAAAAAAGACGAGGATAAAGATAATGAAATTATATATAAGGGGTTATTAAAAATATTAAATTCTGTTCTTTATGATCAATTAGGAATTTATTTAACGATAATAAAAAATATTTAA", "aa": "MAEIITRGYLVARQDYDVFDEILTFINEYGNRFIMFAPGTKRITSKNARALFFGNFLEIQFFYARNDTKLSKLKKVISLDQIDYKYENTYSMLILSELMTKVVNFNVEFYQFYQLILEYIILEYNDYYISCFLLVKFLIMNGVNFNFQACVHCNSSKNIKTFSLADHGLICINCESKIINKIDYEPKALQLWQKLYFATQIKKDEDKDNEIIYKGLLKILNSVLYDQLGIYLTIIKNI", "aa_hexdigest": "9fea36cd098768ef22bf5bc91c83b2ac", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C91", "ncbi_nrp_id": "WP_010891799.1", "uniref100_id": "UniRef100_A0A2C9DYJ4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C91", "RefSeq:WP_010891799.1", "UniRef:UniRef100_A0A2C9DYJ4" ] }, "ips": { "uniref100_id": "UniRef100_A0A2C9DYJ4", "uniref90_id": "UniRef90_A0A2C9DYJ4" }, "psc": { "uniref90_id": "UniRef90_A0A2C9DYJ4", "gene": "recO", "product": "DNA repair protein RecO", "uniref50_id": "UniRef50_A0A084F1G9", "cog_id": "COG1381", "cog_category": "L", "kegg_orthology_id": "K03584", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1G9" ], "product": "DNA repair protein RecO" }, "genes": [ "recO" ], "id": "GPKLONFDEH_1018", "locus": "GCA000006625_00997" }, { "type": "cds", "sequence": "contig_1", "start": 590546, "stop": 590650, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATATAAATTTAAAACACAAGAAGACTTAGTAAATCATTTAAAATCTGTTGGTTTTGTTTTTGCAAATTCAGAAATATATAATGGATTAGCTAACGCATGA", "aa": "MRYKFKTQEDLVNHLKSVGFVFANSEIYNGLANA", "aa_hexdigest": "0e7805433988d752c369bb08bc801e76", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3905.3942000000015, "isoelectric_point": 8.161197853088382 }, "id": "GPKLONFDEH_1019", "locus": "GCA000006625_00998" }, { "type": "cds", "sequence": "contig_1", "start": 590999, "stop": 591286, "strand": "+", "frame": 2, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "nt": "ATGTTTAAAACACACCAAGGAGTAATTGAAGATCCTAAAAACATCGTTTATTTAAGACCAGAAACCGCTCAAGGTATTTTTGTTAACTTTAAAAATGTTCAGCGATCAATGCGTTTACACTTACCATTTGGGATTGCACAAATAGGTAAATCATTTAGAAATGAAATAACTCCTGGTAATTTTATTTTTAGAACTCGTGAATTTGAACAAATGGAAATTGAATTTTTCTTAAAAGAAGAAATTGCATATGATGTTTTTGATAAATATTTAAACCAAATTCAAAATTGA", "aa": "MFKTHQGVIEDPKNIVYLRPETAQGIFVNFKNVQRSMRLHLPFGIAQIGKSFRNEITPGNFIFRTREFEQMEIEFFLKEEIAYDVFDKYLNQIQN", "aa_hexdigest": "33ee3a4b30664ce1e237be37a476267a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003241E146", "query_cov": 0.8210526315789474, "subject_cov": 0.8478260869565217, "identity": 0.795, "score": 125.0, "evalue": 1.37e-35, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_X1KVU4" }, "pscc": { "uniref50_id": "UniRef50_X1KVU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_1020", "locus": "GCA000006625_00999" }, { "type": "cds", "sequence": "contig_1", "start": 591311, "stop": 591967, "strand": "+", "frame": 2, "gene": null, "product": "Glycine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A940AN01" ], "nt": "TTGAATAATTTAAGAAAACATGAACATCCCAAAGAAGAATTATCACACTATTCAAAAAAAACAATTGATTTTGAATATAATTTCTTACATGGTTTTTCTGAATTATATGGAATTGCATACCGAACTAATTATGATTTATCTGTGCATATGAATTTATCAAAAAAAGATTTAACATATTTTGATGAAGAAACTAAGCAAAAATATATTCCTCATGTTATTGAACCATCAGTTGGTGTAGAAAGATTATTGTATGCTATTTTAACTGAGGCTACATTTATTGAAAAACTAGCAAATGATGAAGACCGAATTTTAATGAATTTAAAATATGATCTAGCCCCTTATAAAATTGCTGTAATGCCATTAGTTAATAAATTAAAAGAAAAAGCAGAAGCAGTTTATAATAAAATTTTGGATTTAAATATTTCAGTAACTTTTGATAATAGCGGAAGTATAGGCAAACGCTATCGCCGTCAAGATGCTATAGGAACAATTTATTGTATAACAATTGATTATGATTCATTTGGTGATGAACACGATCCTACTTTTACTATTCGTGAACGAAACACAATGGCTCAAAAAAGAATTAAATTATCAGAGTTATCTTTATATTTAAGCCAAAAAGCTCATGAAGATTTTCAAAAGCAATGTCAAAAATAA", "aa": "MNNLRKHEHPKEELSHYSKKTIDFEYNFLHGFSELYGIAYRTNYDLSVHMNLSKKDLTYFDEETKQKYIPHVIEPSVGVERLLYAILTEATFIEKLANDEDRILMNLKYDLAPYKIAVMPLVNKLKEKAEAVYNKILDLNISVTFDNSGSIGKRYRRQDAIGTIYCITIDYDSFGDEHDPTFTIRERNTMAQKRIKLSELSLYLSQKAHEDFQKQCQK", "aa_hexdigest": "b0065846fb2a186c239b3446eaa6c9fa", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0026009322", "query_cov": 0.926605504587156, "subject_cov": 0.8178137651821862, "identity": 0.51, "score": 189.0, "evalue": 1.02e-56, "valid": false, "product": "glycine--tRNA ligase", "ec_ids": [ "6.1.1.14" ], "uniref50_id": "UniRef50_A0A940AN01", "kegg_orthology_id": "K01880", "go_ids": [ "GO:0000166", "GO:0004820", "GO:0005524", "GO:0006426" ] }, "pscc": { "uniref50_id": "UniRef50_A0A940AN01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A940AN01" ], "product": "Glycine--tRNA ligase" }, "genes": [], "id": "GPKLONFDEH_1021", "locus": "GCA000006625_01000" }, { "type": "cds", "sequence": "contig_1", "start": 592099, "stop": 592329, "strand": "+", "frame": 1, "gene": null, "product": "CHC2 zinc finger domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F98474", "UniRef:UniRef90_UPI0002F98474" ], "nt": "TTGTCAATCAATGATAAAAAACAAATCTTTAAATGTTTTGCTTGTAATCATGCTGGTGATGTGATTAAATTTGTTGCTGAATATAAAAAGATCTCCTATGCTTTAGCAGCACAAGAGATTGTTCAATTAATGAATTTAGATTTAAACTTATTGAATCATTTACAAAAAATTTCTCCACAAGAAATTAAAAAAAATAAGGTTTTTGATTTAAACAAACAAATTCAAAAATGA", "aa": "MSINDKKQIFKCFACNHAGDVIKFVAEYKKISYALAAQEIVQLMNLDLNLLNHLQKISPQEIKKNKVFDLNKQIQK", "aa_hexdigest": "322679980a97ee7673b1bb394c6f9ee7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 591955, "stop": 592309, "observations": { "insertion": [ 592285 ], "mutation": [ 592060 ], "stop": [ 592060 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002F98474", "query_cov": 0.8421052631578947, "subject_cov": 0.5470085470085471, "identity": 0.9840000000000001, "score": 126.0, "evalue": 3.26e-36, "gene_start": 1, "gene_end": 64, "reference_start": 49, "reference_end": 112, "reference_sequence": "MSKITNNVFLTIRNAVNISNVIQNYIKVIKKGNNYWAICPFHNDTNESLSINDKKQIFKCFACNHAGDVIKFVAEYKKISYALAAQEIVQLMNLDLNLLNHLQKISPQEIKKIKFLI" }, "paralog": false, "identity": 0.9658119658119658, "score": 222.0, "evalue": 2.02e-76, "description": "Internal stop codon at 592060. Frameshift due to insertion around 592285. Nonsense mutation around 592060.", "psc": { "uniref90_id": "UniRef90_UPI0002F98474", "product": "CHC2 zinc finger domain-containing protein", "uniref50_id": "UniRef50_UPI0002F98474", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002F98474", "UniRef:UniRef50_UPI0002F98474" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002F98474", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F98474" ], "product": "CHC2 zinc finger domain-containing protein" } }, "id": "GPKLONFDEH_1022", "locus": "GCA000006625_01001" }, { "type": "cds", "sequence": "contig_1", "start": 592777, "stop": 593715, "strand": "+", "frame": 1, "gene": null, "product": "toprim domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002100652A", "UniRef:UniRef90_UPI002100652A" ], "nt": "ATGGATGTTATTACAATTCATAAATCTAACAAACCAAACGTTGTAGCAACAATGGGTGTTGAATTAACTAATAAACATTTAGAATTAATTAAAAGCTATGGAATTAAAGATTTAATTTTATGTTTTGATAACGATCAAGCAGGTTTTTTAGCTACTAAAAAACAAATTATGAAATTAATTAATTCCCCATTCAACATTTTTATTGTTAATCATAAAGATAATGATTGCAAAGACATGGATGAATATGCAAATAAGCACGGTTATAGTGCAACAAGTAAGCTATTAGACCAACAACTTCATATTAGTGAATTTTTATTATTAGAAATTAGTAATACAAACAAATCTACCCCCTCACTAAAACACAGTTATAAACAAGAGTGTTTTACAATCATTAAAAAATATGGTGATATTTTTTATGTAGATAAATATGTTGATTTCATGAAACAAACATTTTCAATTGAAGAAAATTTATTAAAAAAAGTTATTGAAGAATTATTAATTAACAAACAATGTAATTCTATTCAAAACAAAAAGAACATTCAATCATATCAAAAACAAACACAACTAACTAATAAACTTACTACTTCAAACCCAAAAGTTATCCTAACAAAAATTGATAAACATTTTAACCACTTAGTAGTAATTATATTAATGAATCGGGTTTTCTTAAACCGTCTTGATACGCAAGTTCGTAACCGGTTAACTAAAGAGCAAAGTTTAGTGATTAATATTATAGAAGACTTTTATTTGTACCATTTTAAGATTAATAATATTTTAAAAAACACTGATTCTTTTATTGAATTTATTAATAAAAACTACAGTAAATATACTAATGTTATTAACCAACTTACAAAAATGGTGCAGATTATTAAATATAACCCTAAGTTTGATAATATAGTGGCATTTGAACTAGCTAAAAATAATTTTATATATGAATGA", "aa": "MDVITIHKSNKPNVVATMGVELTNKHLELIKSYGIKDLILCFDNDQAGFLATKKQIMKLINSPFNIFIVNHKDNDCKDMDEYANKHGYSATSKLLDQQLHISEFLLLEISNTNKSTPSLKHSYKQECFTIIKKYGDIFYVDKYVDFMKQTFSIEENLLKKVIEELLINKQCNSIQNKKNIQSYQKQTQLTNKLTTSNPKVILTKIDKHFNHLVVIILMNRVFLNRLDTQVRNRLTKEQSLVINIIEDFYLYHFKINNILKNTDSFIEFINKNYSKYTNVINQLTKMVQIIKYNPKFDNIVAFELAKNNFIYE", "aa_hexdigest": "037ef31637afe4ed8138d9c109be76fc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002100652A", "query_cov": 1.0, "subject_cov": 0.8387096774193549, "identity": 0.9490000000000001, "score": 584.0, "evalue": 2.43e-209, "valid": true, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_UPI002100652A" }, "pscc": { "uniref50_id": "UniRef50_UPI002100652A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002100652A" ], "product": "toprim domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_1023", "locus": "GCA000006625_01002" }, { "type": "cds", "sequence": "contig_1", "start": 594490, "stop": 594666, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGTTTCAATTGTTGCACATAATAATTGTTTAGAATTTAAAAATATTCAATTAATTCCAAACCAAAATTATAATATAGTTAAAGTCTTACACTATCAAGCATCAAATACAATTTCAGCTAAAGATATGGGCTATAGTTTAAAAAATGCATTTAGTACCAAATCAGTCGATTGA", "aa": "MIVSIVAHNNCLEFKNIQLIPNQNYNIVKVLHYQASNTISAKDMGYSLKNAFSTKSVD", "aa_hexdigest": "8e1756445f9b715ad49b46f730b2fa33", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6529.435400000002, "isoelectric_point": 8.891108894348147 }, "id": "GPKLONFDEH_1024", "locus": "GCA000006625_01003" }, { "type": "cds", "sequence": "contig_1", "start": 595084, "stop": 595374, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGATAATCTCAAAATAATTAATATTGATAATCGCATAACAAAAACTATTAAAACAGGTATTCATATTGATGTTAATGAAGTGCAAAATCTTGCGCAATTTAAAGATGGAATTTCAAAACAATTTAAATTAGTTGGAAAAAATGATAGTACAGTTTTAAACCAAATTTTAAATGGTGATTTTTCTATAAATATTGAATACAAACGAAAAGATCAACCTGATAATGAAGCTAGTTTTAGTACTGGAGCATGTAAATTATTTAAGAAAAATAATAATGACCCATTTTGA", "aa": "MIDNLKIINIDNRITKTIKTGIHIDVNEVQNLAQFKDGISKQFKLVGKNDSTVLNQILNGDFSINIEYKRKDQPDNEASFSTGACKLFKKNNNDPF", "aa_hexdigest": "daee0dcee61363a75c9f6d55aaab0237", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10900.244299999988, "isoelectric_point": 8.733612632751463 }, "id": "GPKLONFDEH_1025", "locus": "GCA000006625_01004" }, { "type": "cds", "sequence": "contig_1", "start": 595537, "stop": 596055, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTTCAAATTCGATATGACGATCCAGATGACAGTTTATTTATGGGGCAAAGAATAGGTATAGAAAAAATTTTACTGCCAAATAAAAGAAAACAAATACCTTTGAACCCTCCATTAGTTGGTGAAGTTGATAACAAGAAAAAAATCATTGAATTTAATATTGATAATAATGATGATCATTTTAAACCAGGTAATTTATATCAACTTATTAGCATTGATTTAGTTTATGAACCTAAGACACTATCCCATAAGGATTTAAATAAATTAATGTATGAAAATCCACAAACAAAAATGTATAAGGATAAAGTAACTTTTGAATTAGACAATAGTCAATACTCAAGTATTAAAATTGAAGAAAACCAAAATCCTTATGAAGTAAAAAGTGTGAAAAATAAATTAGATAATAAAAATCTAACACTAAAATCAACTGTGACATTTAACAATAAAGATGAACATCTTTATAATAAATACATGTTTGCTTTATTTACTAACACTAAAACAAATGCAGAAGTTTGA", "aa": "MTFQIRYDDPDDSLFMGQRIGIEKILLPNKRKQIPLNPPLVGEVDNKKKIIEFNIDNNDDHFKPGNLYQLISIDLVYEPKTLSHKDLNKLMYENPQTKMYKDKVTFELDNSQYSSIKIEENQNPYEVKSVKNKLDNKNLTLKSTVTFNNKDEHLYNKYMFALFTNTKTNAEV", "aa_hexdigest": "4b5143615764e19fe49090e574df22e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20256.817700000018, "isoelectric_point": 6.84087963104248 }, "id": "GPKLONFDEH_1026", "locus": "GCA000006625_01005" }, { "type": "cds", "sequence": "contig_1", "start": 597166, "stop": 597687, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTCCAACTAATTATTCACTATTAACACAAGAAGTTAATGAAAATGGAAATAAAGTTAAAAAAGCATTAGGGGCTTTAGACTTATGTTTTGAGTTGAATGATCCAAGTAATATATTAACTAATCAAGATCAATTTAGTATTAAATACGCAATGTTAAATAATCTAAAACAAAGCGTTTCAGCTAGTGCACATGTTGAAATTAAAGAAAATAAAAAAATAGTTAAATTTCATATTAGTGGAATTGATCTAAATCAAGATTATAAAGTTTGTGAACTAATGTTGTTAACAAAACCAGCTAGAATGGGTGAAAACATCAATAATAATGATAAAAACAACTATTCATCTATTTATCAAAATTCAACAAATGATGATAATCAATATAAATTTAAAATTCTATTAGAAAATAATGATTTGTCAACTAAGGAACGTCCATCAAAAACAGGTGATGTTGTTAATAATAAAGGTAAGGTTGATATTACAATTACACAAGCTCCAAAATTATTAGCTAATAAATGA", "aa": "MQTPTNYSLLTQEVNENGNKVKKALGALDLCFELNDPSNILTNQDQFSIKYAMLNNLKQSVSASAHVEIKENKKIVKFHISGIDLNQDYKVCELMLLTKPARMGENINNNDKNNYSSIYQNSTNDDNQYKFKILLENNDLSTKERPSKTGDVVNNKGKVDITITQAPKLLANK", "aa_hexdigest": "3d5a176b71b0a5f3b5475048dea96a07", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19526.853700000007, "isoelectric_point": 8.403598976135257 }, "id": "GPKLONFDEH_1027", "locus": "GCA000006625_01006" }, { "type": "cds", "sequence": "contig_1", "start": 597943, "stop": 598191, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATATGAACTAAAAGATGCTAAATTAATATTTAATGAAGAAGAATTGAAGAAATTTAACAAGGTAACAGCAGTTGATAATTTAAATGTTGATTCTGATCAAATAGTGTTTAAAACAATGAAAAGTGCTTATGTTGAAACTGCAACGATTAATTTTGTAAATAATACTCCAAACGCTACAATTGATCTTAAACTAAATGGGGATTTAAGTGTATTTAGTAAAAGTCAATATATTCGTTTTGAGTGA", "aa": "MQYELKDAKLIFNEEELKKFNKVTAVDNLNVDSDQIVFKTMKSAYVETATINFVNNTPNATIDLKLNGDLSVFSKSQYIRFE", "aa_hexdigest": "5f40922f752d3200ad24133e8bc3131c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9442.5996, "isoelectric_point": 5.068924140930174 }, "id": "GPKLONFDEH_1028", "locus": "GCA000006625_01007" }, { "type": "cds", "sequence": "contig_1", "start": 598273, "stop": 598548, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00331586B6", "UniRef:UniRef90_UPI00331586B6" ], "nt": "GTGGGTTTAGCAAAAAATAATGTGAGTGATAATACTGATTTTAAAAAATATAAATTAGAAAAGGGTTATCTAACAAAGAAAACAGATGATTTAGATCAATTTACAGTTCGTGCAAGTAAACCTTTAAAAATTAAGCAAGTAAATGTTAGTGAAATCAAAGCACATCATGCAAAACTAAATCTAAAACTTGAAGACCAAGATAGTGTTTTAAAAAATTTAAATTATGAGCTTGAATTACGTTTTGGAGTAAAAAATAATCAATCATCTAATTCATGA", "aa": "MGLAKNNVSDNTDFKKYKLEKGYLTKKTDDLDQFTVRASKPLKIKQVNVSEIKAHHAKLNLKLEDQDSVLKNLNYELELRFGVKNNQSSNS", "aa_hexdigest": "d663cb9ecbf5f2527d13ce4465a42faa", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00331586B6", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.912, "score": 156.0, "evalue": 5.04e-48, "valid": true, "uniref50_id": "UniRef50_UPI00331586B6" }, "pscc": { "uniref50_id": "UniRef50_UPI00331586B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00331586B6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10439.741199999995, "isoelectric_point": 9.455722999572757 }, "id": "GPKLONFDEH_1029", "locus": "GCA000006625_01008" }, { "type": "cds", "sequence": "contig_1", "start": 598549, "stop": 598806, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTAATAAAATTGAGATTAACAAAAAAGCTCAAGAAAACAGTTATACCCTAAAATTAACTTTAGATGGTGATTATTCTGAATTTATTAAACAAGGGTATTTACGTTTATTATTAGTAAATAATAAAAACGAAGAGTTTAATTATACAAAATCACATAAAGATACAGATAAAAATCAAGATAATAATGCTGTTAAATACTTTACTTTTGACCTTAATAATTTAACATGGGGAATGCAATATACAATTAAATGA", "aa": "MFINKIEINKKAQENSYTLKLTLDGDYSEFIKQGYLRLLLVNNKNEEFNYTKSHKDTDKNQDNNAVKYFTFDLNNLTWGMQYTIK", "aa_hexdigest": "c474079ab0d3821fece815be2a44f143", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10167.325399999994, "isoelectric_point": 7.886433601379394 }, "id": "GPKLONFDEH_1030", "locus": "GCA000006625_01009" }, { "type": "cds", "sequence": "contig_1", "start": 598882, "stop": 599049, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAATACAAAAATAAATCCAACTACTCCAAAACAAAAAAACATACATGTTAGCGTTAAAGAAATTCAAAATAATGAAGTAATTTTAAATGATGTAGATCTTAGCGGATTAAGTGAAAATACAGAATATGAACTAAAAACGGTTAGTTTAAATCAAGAAAATTGA", "aa": "MFNTKINPTTPKQKNIHVSVKEIQNNEVILNDVDLSGLSENTEYELKTVSLNQEN", "aa_hexdigest": "ad6307150ca673fdea980e2007c278b5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6272.912699999999, "isoelectric_point": 4.83730525970459 }, "id": "GPKLONFDEH_1031", "locus": "GCA000006625_01010" }, { "type": "cds", "sequence": "contig_1", "start": 599749, "stop": 600771, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGATTGGTCATGACAAATTAATTGCTAATAAGGCTTATGAAATCCAAGCTATTTATTTCTCAGACCAAAATAATGATTTTGCATGCACAAACACAAATCAAATTCCAATTAATTCATCAATTACAAATAAAAAAATAGTTATAAATCCATCAGCAACAACAATTAGTTCACATGAAGTTAAAAATATTACTTCAACATCAGCTGATATTAATTGTTCTATAAGTACAAATGATGGTGATATTTTTAAACCAAATTCACAAATTATAGGTCATTTTGCTAAAGGTGATGATTTAACTAAGACTTTTGTAGCTAATGGTGTTGTTGCGAATGTAAATAACCAAACAAAACTTAGCTTTACGTTAAATAATTTAGAACCAAATACTAAATATATATTAGTTAGTGTTGAATTTAAACAACAACCAAATAATGCTTATAAACATATTAATAACAAATATAATGATCAGTTTTATTCAAATGATCAAACACATTCAATTATTTTTACAACAAGTACACCTATTTTTGAAATTAATAGTATTAAAACTATAAAAAATACTTACGCATCAAATAAAGAAGAAATTAAAGTTAACGCTAAATTTAATAAAATTGATCCATATTTAAATGCTAAAAAACTAAAATTAGTTTATCAATCACAAAATAATAACGAAACCTTTGAAACTCAACCATTATTAGTTAGCACAAATACACATGATTATGTGTTTAGTTTTGATAAAAACGCTTCAAATTCAATTATTGGTAACCGTGAATATCATTTAGATAAAATTTTATATTTAAATGAACATAACCAATGGTGTGAATTAAATAAGAATAATCTTGTTGACCAATTTAATGTTGAACCTACACCAATTAGTATTGCAAAAATTGTTGAAGATGATTCAAAAAGAACACAAAACGATGTTAAATTTAGAATTTATTTTAATGACTTAAATCAAGATCAAATCTTTAATGATAATGATAATGTTAATGTTAAGTTAGTTTATCCAAATACAAACAATGTATGA", "aa": "MEIGHDKLIANKAYEIQAIYFSDQNNDFACTNTNQIPINSSITNKKIVINPSATTISSHEVKNITSTSADINCSISTNDGDIFKPNSQIIGHFAKGDDLTKTFVANGVVANVNNQTKLSFTLNNLEPNTKYILVSVEFKQQPNNAYKHINNKYNDQFYSNDQTHSIIFTTSTPIFEINSIKTIKNTYASNKEEIKVNAKFNKIDPYLNAKKLKLVYQSQNNNETFETQPLLVSTNTHDYVFSFDKNASNSIIGNREYHLDKILYLNEHNQWCELNKNNLVDQFNVEPTPISIAKIVEDDSKRTQNDVKFRIYFNDLNQDQIFNDNDNVNVKLVYPNTNNV", "aa_hexdigest": "e236559dfb2bbfd2d51970707fad3f9d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38818.6335, "isoelectric_point": 5.917189216613769 }, "id": "GPKLONFDEH_1032", "locus": "GCA000006625_01011" }, { "type": "cds", "sequence": "contig_1", "start": 600901, "stop": 601509, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAATCACAAATTACACCTAATCATCAATATGAGTTGAGTGAAATTTGTGCAATTAAAAACAAGGGTGCAAACAATGAAATTAAAAAAGATTTAAATGAAAATCATCTATCATTAGCTTTAGGGTTAGCGCCAAATAAAATGGATGTGCAAAAACCAACAATTACAATTACAAAAGTTCAAGATAATCAATCTGTTAGTGCACATGTTAAATATATAATTAATGATCCTGATGGTATTTTAAATTCAACTGATTGCTCAAAAGAATTATTAAATGTTAAATATGCTTTATTTAATCAAACGCAAAATGCTAATTTAATTAACAATATTACAATTAAATATGATCAACAAAATAAACAATTCTATTGTGAATTTGATATTGATAATTTAACTTTAAATCAAGATTATGGTATTTTTGAAATTAGTTTTGCTAATAAACCAATTCATGCCGCATTTGCTAAAATTAATGATACCCAACATCCTTTAGCTCTTTTAAGTACTAATAATCAAGATATAAATCAAAAAATTGCTTTAGATTGTGAAGTTAATAAAGTTACAATTAATAAACAAGATCTTGATTATGATCAAAACACCAAGATTAGTTGA", "aa": "MLKSQITPNHQYELSEICAIKNKGANNEIKKDLNENHLSLALGLAPNKMDVQKPTITITKVQDNQSVSAHVKYIINDPDGILNSTDCSKELLNVKYALFNQTQNANLINNITIKYDQQNKQFYCEFDIDNLTLNQDYGIFEISFANKPIHAAFAKINDTQHPLALLSTNNQDINQKIALDCEVNKVTINKQDLDYDQNTKIS", "aa_hexdigest": "05475d606c6c0aca030b1aec0f2eef55", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22883.50200000001, "isoelectric_point": 5.487145423889159 }, "id": "GPKLONFDEH_1033", "locus": "GCA000006625_01012" }, { "type": "cds", "sequence": "contig_1", "start": 601954, "stop": 602586, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAAAGTTTGGTTCACAAGCAAACCAAGTGATACTATCTATAATGGCGTTAATAAGGACAATATAGTTTATCCAATTAATAATCATAATACTAATATTGATTTAAAAACTTTAGAAAAATCAACTTTAAATAGTGTTAAACCAACAAAAACAAACTTTAACAAGAATGAATCAATTAAGTTAATGGTTGGTTTTAATAAAACAGGTTCTAGTTTAGAAAACAAATATGCGAAATTAGTTTATAAAGATAATAATAACCAACAAATTGAATCAGATCCTGTATTGTTGAATTCAGATACAACTACAAAACCATTTAATAAAGAATTTAGTTTTAATAATAATCAAACTAGCTTAGTTGCTAATCGTGATTTTGAATTTGTTAAGTTAATCATTAGTGATACTGCTGATTTTAAAAACGCTAATACACTTGATTTAACACCTAATTTTAATAAAGATGATGCTAAATTTAGTATCGAACCTACACCTATTAGTGTTAATAATGTTATTCAAAGTGGATCAAATACGTATGATGAAATCCATCTATCATTCGATTATGATGATCAAGATCATAATTTAGTTGATAATGACCAAATTACAATTACATATCGCAAAAAAGGTGAACAAGCTTGA", "aa": "MKKVWFTSKPSDTIYNGVNKDNIVYPINNHNTNIDLKTLEKSTLNSVKPTKTNFNKNESIKLMVGFNKTGSSLENKYAKLVYKDNNNQQIESDPVLLNSDTTTKPFNKEFSFNNNQTSLVANRDFEFVKLIISDTADFKNANTLDLTPNFNKDDAKFSIEPTPISVNNVIQSGSNTYDEIHLSFDYDDQDHNLVDNDQITITYRKKGEQA", "aa_hexdigest": "b39b5bb386819f2328d928f4e80a0bc6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23949.185200000007, "isoelectric_point": 5.417403984069824 }, "id": "GPKLONFDEH_1034", "locus": "GCA000006625_01013" }, { "type": "cds", "sequence": "contig_1", "start": 602635, "stop": 602901, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGTTATTAAAAATAATGTAAACCCACAAACAATAAGCACTAAACCATCAAGTACGTATGTTGATTTAAAAGCACAAAATGCAGATGAAAATAAAATCACTTTAAGTTTATTGTTACATTCAAATGACCAAATTTTTGAAGATAAGAATAACCATCTAAAAATCGCTAAAATCAGTATTGATGAACTTGATGCACATGATCAAATAATTAATTCAACAACGCATGATTATGATTTAACATTAGAAAAAGAAAATAATGAATGA", "aa": "MVVIKNNVNPQTISTKPSSTYVDLKAQNADENKITLSLLLHSNDQIFEDKNNHLKIAKISIDELDAHDQIINSTTHDYDLTLEKENNE", "aa_hexdigest": "34afd17d9d833c68099bb624991a7a99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10003.95429999999, "isoelectric_point": 4.865554237365722 }, "id": "GPKLONFDEH_1035", "locus": "GCA000006625_01014" }, { "type": "cds", "sequence": "contig_1", "start": 603769, "stop": 604254, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAATTAATTTTAGATGATAGTGATAATCAACTACATAGCGGTGATGAAATTAATATCAATTATCGTATTAAAGGAACACAAAATATTATTTCAACCCCAGCTACAATTAGTGATCAAGATAAAAGATATACAAAATGTATGGCAACTAATTTAATTGCTGGTCTTGATTATGAAATTGTGAGTGTTACAATTAAGAATCAAAAAACCAAGAATGTTTCTCCTATCATTTTTGAACTACCATCAGGACCAATTGGTTTTTATACTCTTGCCCCTGTTTTTAAAATAACAAGTCTAAGTATGGATCCTGTTTATCATGAAAATAAAAACACAGCAGATTTAAAAATAAATTTACGAATCGAAAACATTGGCGCTTCTTTATTTAATAAAGATATCAAATTTATTTTCAAACGTAAAAATGATGGTAAGCAAATTTCATTTATTCATAAAGTTACATCAGCAAATGACGCAAATTATGAATGA", "aa": "MIKLILDDSDNQLHSGDEININYRIKGTQNIISTPATISDQDKRYTKCMATNLIAGLDYEIVSVTIKNQKTKNVSPIIFELPSGPIGFYTLAPVFKITSLSMDPVYHENKNTADLKINLRIENIGASLFNKDIKFIFKRKNDGKQISFIHKVTSANDANYE", "aa_hexdigest": "7059d30b5e03d54b87427befa0f0680f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18155.568099999993, "isoelectric_point": 8.558709907531739 }, "id": "GPKLONFDEH_1036", "locus": "GCA000006625_01015" }, { "type": "cds", "sequence": "contig_1", "start": 604267, "stop": 604944, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "ATGCTTGTTGAATTATGTTTTGAAAATTTAGTAGATAAAAACCAACCAGTTTTAAAAACACAAACAACATTAGTTAAAAAAGATGAACATAGTTTTAGTGCTTCAGGGGTAATTACTAATTTATTAGCAGATCATACTTATCGTTTAACAAGTGTTAGTTTAAAACAAAAACCACAATTAGCTAATGTTAATATTAATAACAACAATAATAATGAAATTCTTTTAAACAATATTGAAGATAATCAAAAAACTATTCATACATTATCAAAATCAATTGTTAATGAAATTAGTAATTTAGCAGATGCTTATCCAAAAAATAATAATGGCATATATGATGTTAAATTCAATATTAATATTATTAAGAACAATAATAAGCTTAATAATAAATATGTTAAAGTAGTATTTGAGGACAATGAACACCAACTAATAAGTACAAACGATCTTTTAGTTAATAAATTAGACCAAACTAATTTAATTAGTTTACAAAATTTTAGTTTTAGTAATTTAAAACCAAATCACCTTTATCGTTTATTAAAAGTTGTTTATGGTGATGAACAAAATTTTGATGCTATTAATGAACAAAAAAATATTTTAGCTTTAAATCCCTCTTTAGTTAATTCAAGCTTTAGTACTACGCCAGCAAAAATTAAAGTTAGCAAAAACGCTATTGATGTATGA", "aa": "MLVELCFENLVDKNQPVLKTQTTLVKKDEHSFSASGVITNLLADHTYRLTSVSLKQKPQLANVNINNNNNNEILLNNIEDNQKTIHTLSKSIVNEISNLADAYPKNNNGIYDVKFNINIIKNNNKLNNKYVKVVFEDNEHQLISTNDLLVNKLDQTNLISLQNFSFSNLKPNHLYRLLKVVYGDEQNFDAINEQKNILALNPSLVNSSFSTTPAKIKVSKNAIDV", "aa_hexdigest": "665feb9a415cfee9144d9eeb25e7c504", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 65, "aa_cov": 0.28888888888888886, "hmm_cov": 0.8923076923076924, "evalue": 7.507924754976924e-09, "score": 31.489885330200195, "start": 123, "stop": 187 } ], "seq_stats": { "molecular_weight": 25465.50930000002, "isoelectric_point": 7.788892936706543 }, "id": "GPKLONFDEH_1037", "locus": "GCA000006625_01016" }, { "type": "cds", "sequence": "contig_1", "start": 605242, "stop": 605997, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATAACGATGGGGAACATGATTTAGTATCTAATAACTTTGTTGTTTTAAATTATGGTGTTAATGAATATGATTTTAATTTAGATGATTTAAAACATAATCGTATTTATTCATTTAAAAAACTGATTTATACTGAACCTAATCAAAATGATTATAAATTTAGTTTTTTAAATAATCAAAAAATTACAAACACTTTTAAAACAGCATCAGTTAACGAGCAATTAACAATAGATACTAAACTAGTTCGCAAACCAGATTCAAATTTAAATAAAATTAATTTAAAACTTCAAATAAATGATCCTAATGATTTTTTAGAACAAGATAGTATTTTAGAAATTACATTCCATGATGAAAAAGATAAAACAACTCATAATGTTATTGGTAAAATCAATGTTGACGCTAATAATAATAAAACACTTGAATTTAGTGTAGAAAACACTAACACATTTAAAATTCAACCAAATCATAAATACATTGTTGATAATATTAATTATGCAACAAAAAATAAAATTCAACCTGCCAATGCTATTAGTAATAATTCTAATAAAAATAGTATTTATGATGCTAGTTCAAATCCAAGTAAAATTCTAAGTTTTACAAACGAATTATTTGTTAACAACATTAATGTTAATCAACCTAATACAAATTTAAATCCTACTGCAAGCATTGATGTTGAATTAAAATCTAGTCAAAACTTATTAAAAGACCAATATTTACGAGCTTTATATATTGATAATAACCACCAAAAAATTTGA", "aa": "MYNDGEHDLVSNNFVVLNYGVNEYDFNLDDLKHNRIYSFKKLIYTEPNQNDYKFSFLNNQKITNTFKTASVNEQLTIDTKLVRKPDSNLNKINLKLQINDPNDFLEQDSILEITFHDEKDKTTHNVIGKINVDANNNKTLEFSVENTNTFKIQPNHKYIVDNINYATKNKIQPANAISNNSNKNSIYDASSNPSKILSFTNELFVNNINVNQPNTNLNPTASIDVELKSSQNLLKDQYLRALYIDNNHQKI", "aa_hexdigest": "aa14f0f91f5811b5444efa65bbf08197", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28983.768300000036, "isoelectric_point": 5.869501304626463 }, "id": "GPKLONFDEH_1038", "locus": "GCA000006625_01017" }, { "type": "cds", "sequence": "contig_1", "start": 606286, "stop": 606525, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAATATAACACCTTTACAAATTCAAACAGCTTCAAAAATCAATCTTAATTCACCAAAAGTAACTATTAATAACATCACTCCTGCTAGTGCGGATTTATATTTACCATTAGTGTCAACCGATGATATTTTTGCAAAAGATCAAGTTATTGATGTTACTATTGGCAATAAAGATAATACGAAAAATGATTTAATGTTTACATCTAATTTAGAATATGATGATATAACAAAAACGTGA", "aa": "MNNITPLQIQTASKINLNSPKVTINNITPASADLYLPLVSTDDIFAKDQVIDVTIGNKDNTKNDLMFTSNLEYDDITKT", "aa_hexdigest": "d8c17e9bec3a23170b6f70ea3cd756e5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8743.7441, "isoelectric_point": 4.295345497131348 }, "id": "GPKLONFDEH_1039", "locus": "GCA000006625_01018" }, { "type": "cds", "sequence": "contig_1", "start": 606682, "stop": 607269, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAAAAAATAGCACTGATTTAAGTCATGGTTTTAAAACAGAACCAACAGGAATTATTAGTTTAAAAAATAATAGTTTACAAGTAATTAAACGTACTGATGATAGTATTAGATTATCAATTCCATTAGTTGATAATGATCATGTCTTAGTTGACAGTGATGGTAGTTCATTTACTATTAAAATTAGTGATTTAGATGATCCTAATAATCTTTTTGATGCAGATAATCAAAAAATAGTGATTGATCAAAACGGGAATCGTAATTTAGAGTTTGATGTTCCTAATGTTTCATTAAACCATAAATATCATATTAACAATATCACGTTGATTAATAAACCAATCAACGCTGCTTTTAATCAAAATGAAAATCATGAACAAGTTTATGAGTTAAATAATTCTCAACCACAATTGAGTGAATTTGGTAATGAATTATTAGTTAATACTTATAATTATGAGCCAAATATAACAGATGTAAAAACAAATGTTTTAAATTATAAAGTTAAATTAACCCTTAAACGTTCATTGAATTTAATGAATGAATACTATTTAAAACTAAAGTATATTGATAATAATGGTGATGTTGTTTGA", "aa": "MIKNSTDLSHGFKTEPTGIISLKNNSLQVIKRTDDSIRLSIPLVDNDHVLVDSDGSSFTIKISDLDDPNNLFDADNQKIVIDQNGNRNLEFDVPNVSLNHKYHINNITLINKPINAAFNQNENHEQVYELNNSQPQLSEFGNELLVNTYNYEPNITDVKTNVLNYKVKLTLKRSLNLMNEYYLKLKYIDNNGDVV", "aa_hexdigest": "91183b4dec1645ee97c516b3d59698a2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22279.575900000014, "isoelectric_point": 5.030330467224121 }, "id": "GPKLONFDEH_1040", "locus": "GCA000006625_01019" }, { "type": "cds", "sequence": "contig_1", "start": 607462, "stop": 608976, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "ATGATGGTCAAAAAAGTAATTAATGTTGCTGATTTGGATCAAAAACAAATTATTGTTAACAAACTAAATATTGGTAAAATTGTTAATAACCAATTAGTTTTTGATGTGCCAAAAAATGTTGAAGAAGGCGAAGACGTTTTTGTTAATTACCAAGATGTTGATGGTAATTTGATAAAAATTCCTGCAATTGTTAATAATAAACACCAACTACAAATTGATCTATCAAACAACCCTAATTTTACAAAAGATAAGATTTATCATTTTGATTCAATTAGCATAGAAGATGAATATCACATTCCTAAAGAAACAATTATTAGTTCAGATGATGTTGATAATTCAGTTAAAACATTAAATAATGAAGTTGATCTGGGCCGTAAAATTATTGTTAAAAATCCTAGTGTTATTCAAAACAAACCCACAACTTTAATTTTAACAGTTAATGACCCTAATAAATTAGCTTTAAATGAACGATTAAAATTAACACTTGCTTTAAAAAACGATCCAAAACATTGTGTTAGTGCTTATGGCTTAATTGATTATAAAAATAATAGTTTAGTTTTTGATTTTAATCATTTACAAACAGATACTGCTTATTACATTAAAAATTTAGTTTTTAAAAATAATAATTTAAATCTTACTGATTTAAACGCTGAAGCTCATAGTCAAACTAAAATCAATATTATTAAAGATCAAGTTATTGGTTATACATTTAGAATTTTTTCTACTAATACATTGGTATTAGAGGATTATCATCAATATGATACTGATCCTAATGCAACTAAATTAAATTTAAATATTAGTACAACTAATAAGCACCCCAAAACAATTACTTATCGTTTATTAGATGAAAATAATAACGCATTAGAACCAGATTTAGTTGCTAACCTAAATTATGATTTAGTGAAAAAAAATTATGTAGCACATTTGCAAAATTTAAAAGGCAATAGTAAATATTTATTGTTGGGTTTTAATGATGAAAACAACAATAAAATTCATTATTCAACTCCAATTAAAACTCCATTAATTTTTGGTACAACTCCACATGCTACTATAGTTAATTTTGATGTTAACAATCCTAAACAAATTATTAAATCACAAGATGCACGAATAGCAATTGATTTTGTAGATCAAGATCAAAAATTAAGAATTGGTAACCCAGTTGAATTAACTTATTTAAATAAGAATAATCAACAAGTTTATAAAACAATAGGGTATGTTAAAGGAATTAACAAAACCAACAAAAGTGGTACGTTAGAATTTGAACTTAATAATTTAGTATCTAATAATAATTATGAATTGTTAGAATTAAAGGCCTTAGGTTTAGCAAATGATGCGTATCAAAAAGTTGTATTTGCCCCTTTTGATCAAAAACATAATCAATTTAGTACGAAATTAATTGGAACATATATTGAATCAATTAGTTCAAATGAAGTAATTAAAAAAGATGAAAATAAACTTGATGTTAATGTAAAAATTGTTTTCTCACAAAAAGATAAAACATTAAATAACGTTTGA", "aa": "MMVKKVINVADLDQKQIIVNKLNIGKIVNNQLVFDVPKNVEEGEDVFVNYQDVDGNLIKIPAIVNNKHQLQIDLSNNPNFTKDKIYHFDSISIEDEYHIPKETIISSDDVDNSVKTLNNEVDLGRKIIVKNPSVIQNKPTTLILTVNDPNKLALNERLKLTLALKNDPKHCVSAYGLIDYKNNSLVFDFNHLQTDTAYYIKNLVFKNNNLNLTDLNAEAHSQTKINIIKDQVIGYTFRIFSTNTLVLEDYHQYDTDPNATKLNLNISTTNKHPKTITYRLLDENNNALEPDLVANLNYDLVKKNYVAHLQNLKGNSKYLLLGFNDENNNKIHYSTPIKTPLIFGTTPHATIVNFDVNNPKQIIKSQDARIAIDFVDQDQKLRIGNPVELTYLNKNNQQVYKTIGYVKGINKTNKSGTLEFELNNLVSNNNYELLELKALGLANDAYQKVVFAPFDQKHNQFSTKLIGTYIESISSNEVIKKDENKLDVNVKIVFSQKDKTLNNV", "aa_hexdigest": "3363952c2f7b58bd999ce9302d9cc247", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 63, "aa_cov": 0.125, "hmm_cov": 0.9523809523809523, "evalue": 6.177789462452785e-13, "score": 44.572662353515625, "start": 150, "stop": 212 } ], "seq_stats": { "molecular_weight": 57419.389099999964, "isoelectric_point": 6.649218559265138 }, "id": "GPKLONFDEH_1041", "locus": "GCA000006625_01020" }, { "type": "cds", "sequence": "contig_1", "start": 608954, "stop": 610993, "strand": "-", "frame": 3, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ4", "UniRef:UniRef90_Q9PPZ4" ], "nt": "TTGGTATGGCAACAAAAAATACTTGTCGATCGTCCTAATGTCTTTAAAGTTAATTTTAATAAAGAAGGCGATAAATTTTTTGAAATTAAATTAAACCATGAATTGAGTAATCAGTATTTGACTGCGCATTTTATTAATCAAAATAATGCTAAATATATTCAAAATATTCAGGTTGATCATAATGGTAATGTAATTGTTGGAACTAAACAACTACCTTTAGGAAATTTTTATAAATTCGTAGGTTTAACTTTAAAAGAAGATAATAAAAACACAAAAATTGTTAATTTAGATGAAATTAGTGAACCCATTCGTGGTATTGATAAAACACGCAGTTTTGATGATCAAGGAAACGTTATTATTAACGCACGTGTTTCACAAAATTTAGCTAATCAAAATATTAATGCCTTATTTGAAGATGAAAAACACCAATTACACACTTTAAAGGCAATAGTTGGTACTGATGGTCTTGTGCGTTTATTAACAAAAGATTTAGAAAATCACCATGATTATAAACTAATTCAAATTGTTGATAAGGATAATCATGATGTTATAAAACCTAATAAATTAGATGCGGCACATCAAAATATTGTCCATAAACCAAATAAAATAAAAATTGAAACTGATAAAAATGGTTTACAAAAAGCACTTCATATTCAAGTTGATTCAAGTTTAAATGATCGATTATTAACACTTAGTTTTGAAAACGAAAACCACGATAAAACCAATATTATTGCTAAGGTTAATAATGGTGAAATTACTGTAGATTTAAACAATTTAAAAGATGGACACTTATATAAACTAGATAATATTAGCATTAATAATACCTCTCCAGCAAAACGCGTTGCTAACTTAGATGATTTAAATGAAAGTGCTAAAACTATTAATAAATTAAACACTAATGCATTAAATGATCCATATCATTATGATGATAAAGGGAATTTAATTTTTAATGCTAAAGTAGATTCTAAACAATTCGCTAATGATCAAGTTGAAGCCGTGTTTATTGATGAAAAAAATAATGAACATAAAGTGTTGACAAAGGTTGATCTAAATGGCAATATTGTAATTAACACTTCTGATTTAAACTTAAATCATACATATAAACTAAAACATGTTATTAGTTTAGAAGAGCCAGAAACAAATGTTTTAAATGATTATGAACTTGCAATTAATCAAAAAACAAAGTTTAGTAAACCAGCTGCGATTGTTGGGGCATTAGAAGATAATAATTATAAAATTCAACTTTTAAACCCTGATTTAGCTAATAAGTCTGTTGTCTTAACTTTTGAAGATGAAAATCATCATCCTTATCAATTAACTGGTAAATTAAATGATCAACAAGAATTAATTTTAAGAACAAATGATGATCATTTACTACCTAATAATCACACTTATACATTGACTAAAATTACTAATGTTAATAATCAACCAATCGTTAATGTTAATGATTTAGATAGAAAAGATAGATTATTAGAAAAAGTCGATGTAAATAAATTACAACAATTAAATACATACACCTATGATGATTTAGGAAATTTAATTATTGACATTAATGATAATTTAAATAAAATAACAACTCAAGATGCTGTTGCTCATTTTATTGATGAAAATAATAAAGATTATTTTGTTCCTACTAAAGTTGATTTAAATAAGAATAAAATAAGTATTAACACAAAAGATTTAGATGCAAACCACCACTATCAATTGTTAAATATTGTTGATAAAAACAAGCATGAGCAAATTTTATTAAATTCAAACGAAATTAATCCTTTAAACAGAATTAGCATTATTAAACCACTTGCAATTATTAAACAAGATGCAAATAAAAATTTAAACCTACTAACTTTTGATAAGAATTTGTTTAATCGTTCATTAGATGCAACTTTTATTGATGAAAATCAAAAAGAATATCATGTTAAAGCAAAAACTATTATTGATTCATTAGGTCAAACACGATTTATAACTAGTGATGAGGTTTTACCTAACCATCATAAATATCAACTAATTGCTCTTAAATATGATGGTCAAAAAAGTAATTAA", "aa": "MVWQQKILVDRPNVFKVNFNKEGDKFFEIKLNHELSNQYLTAHFINQNNAKYIQNIQVDHNGNVIVGTKQLPLGNFYKFVGLTLKEDNKNTKIVNLDEISEPIRGIDKTRSFDDQGNVIINARVSQNLANQNINALFEDEKHQLHTLKAIVGTDGLVRLLTKDLENHHDYKLIQIVDKDNHDVIKPNKLDAAHQNIVHKPNKIKIETDKNGLQKALHIQVDSSLNDRLLTLSFENENHDKTNIIAKVNNGEITVDLNNLKDGHLYKLDNISINNTSPAKRVANLDDLNESAKTINKLNTNALNDPYHYDDKGNLIFNAKVDSKQFANDQVEAVFIDEKNNEHKVLTKVDLNGNIVINTSDLNLNHTYKLKHVISLEEPETNVLNDYELAINQKTKFSKPAAIVGALEDNNYKIQLLNPDLANKSVVLTFEDENHHPYQLTGKLNDQQELILRTNDDHLLPNNHTYTLTKITNVNNQPIVNVNDLDRKDRLLEKVDVNKLQQLNTYTYDDLGNLIIDINDNLNKITTQDAVAHFIDENNKDYFVPTKVDLNKNKISINTKDLDANHHYQLLNIVDKNKHEQILLNSNEINPLNRISIIKPLAIIKQDANKNLNLLTFDKNLFNRSLDATFIDENQKEYHVKAKTIIDSLGQTRFITSDEVLPNHHKYQLIALKYDGQKSN", "aa_hexdigest": "0a47516dc0791128b99799a37ea69403", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PPZ4", "query_cov": 1.0, "subject_cov": 0.8434782608695652, "identity": 0.9990000000000001, "score": 1325.0, "evalue": 0.0, "valid": true, "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PPZ4" }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ4" ], "product": "Unique hypothetical" }, "genes": [], "id": "GPKLONFDEH_1042", "locus": "GCA000006625_01021" }, { "type": "cds", "sequence": "contig_1", "start": 611599, "stop": 612210, "strand": "+", "frame": 1, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBZ1", "UniRef:UniRef90_A0A2C9DY09" ], "nt": "ATGAAAAAAAACACACATAAGAAACTTTTGCCTTTAATTTCTATTTTTGTTTTAATCCCTTTGAGTTCACTTGTTGTAATGTGTTCAAAAACTAAAACAAAAATAGTAAAAGAACAAAAGCAAATTACTGCTTCATCGTTAAGATCTACTGATATTGATTTAACATCAATTAACCTTGAAATCTATTTTGCTAAATTTGATGTTAATGATATTTTAGTTAAAAAATTCAGCATTGAATTAGAAGATGATAAGGGAAATAAGATAAATGTTGATATAAATCCCGAATATAACAAAAATTTACAAATGCTAAGTTTTAAATTAGAAAACTTAAAACCAAACACTACATATAAAATTACAAAGTTTAATATCACAGGACATGAGGTTGATCTAACTCAAATAAAAGATTCATTATTCACTACTAAAGCTGAGAATCTTTTACCTGATATCCCTAATTTACCTAATAGTGTTGGTATTGAAATTAAAGATATTAAAACTAATTCACTTAGCAATGATGCAACAAAAGTGAATGTTAATGTCAATTTAGAAATTAACCAATCAACATTAGAAAATCAATATGCCCGATTAGTTTATAAAAGCAATGATAATTCTTGA", "aa": "MKKNTHKKLLPLISIFVLIPLSSLVVMCSKTKTKIVKEQKQITASSLRSTDIDLTSINLEIYFAKFDVNDILVKKFSIELEDDKGNKINVDINPEYNKNLQMLSFKLENLKPNTTYKITKFNITGHEVDLTQIKDSLFTTKAENLLPDIPNLPNSVGIEIKDIKTNSLSNDATKVNVNVNLEINQSTLENQYARLVYKSNDNS", "aa_hexdigest": "a96f64fd89e1376a80a277bdbb3576b6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 611599, "stop": 612513, "observations": { "mutation": [ 612208 ], "stop": [ 612208 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DY09", "query_cov": 1.0, "subject_cov": 0.5384615384615384, "identity": 1.0, "score": 380.0, "evalue": 2.3e-130, "gene_start": 1, "gene_end": 203, "reference_start": 1, "reference_end": 203, "reference_sequence": "MKKNTHKKLLPLISIFVLIPLSSLVVMCSKTKTKIVKEQKQITASSLRSTDIDLTSINLEIYFAKFDVNDILVKKFSIELEDDKGNKINVDINPEYNKNLQMLSFKLENLKPNTTYKITKFNITGHEVDLTQIKDSLFTTKAENLLPDIPNLPNSVGIEIKDIKTNSLSNDATKVNVNVNLEINQSTLENQYARLVYKSNDNSWKLSNTLKITEIKNSNFVLDGLISNRKYLFKELIIGSQSDLNLTNAQTKITTNNELSFTTAPKPVEIKSVLIDTKFDNHPSSLINLKFNDSENNLKENDILKIKYKKVGPNEVIFEKTVRLANNFELLFEIENTKKNEKYEILSLESNSKHGYNVAPSIFNFISNDLRTFEIKG" }, "paralog": false, "identity": 0.993421052631579, "score": 584.0, "evalue": 1.92e-213, "description": "Internal stop codon at 612208. Nonsense mutation around 612208.", "psc": { "uniref90_id": "UniRef90_A0A2C9DY09", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_B5ZBZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DY09", "UniRef:UniRef50_B5ZBZ1" ] }, "pscc": { "uniref50_id": "UniRef50_B5ZBZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B5ZBZ1" ], "product": "DUF1410 domain-containing protein" } }, "id": "GPKLONFDEH_1043", "locus": "GCA000006625_01022" }, { "type": "cds", "sequence": "contig_1", "start": 612186, "stop": 612383, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACTTAGCTCATTATTAGTAGTTATTTTAGTTTGCGCATTAGTTAAATTTAAATCACTTTGGCTTCCAATAATTAATTCTTTAAAAAGATATTTACGGTTTGATATTAAACCATCTAAAACAAAATTACTATTTTTAATTTCAGCAATTTTTAAAGTATTTGATAGCTTTCAAGAATTATCATTGCTTTTATAA", "aa": "MKLSSLLVVILVCALVKFKSLWLPIINSLKRYLRFDIKPSKTKLLFLISAIFKVFDSFQELSLLL", "aa_hexdigest": "94d24ef72d34d2989e38d4af563c2e5a", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7543.281199999998, "isoelectric_point": 10.106337928771971 }, "id": "GPKLONFDEH_1044", "locus": "GCA000006625_01023" }, { "type": "cds", "sequence": "contig_1", "start": 612463, "stop": 612732, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGTTTAATGATAGTGAGAATAATTTAAAAGAAAATGATATTTTAAAAATTAAATATAAAAAAGTAGGACCAAATGAAGTCATCTTTGAAAAAACAGTTCGATTAGCTAATAATTTTGAACTATTATTTGAAATTGAAAATACAAAAAAGAATGAAAAATATGAAATCTTAAGTTTAGAATCAAACACTAAACATGGATATAATGTTGCACCAAGTATATTTAATTTTATTTCTAATGATTTACGCACTTTTGAAATCAAAGGTTAA", "aa": "MKFNDSENNLKENDILKIKYKKVGPNEVIFEKTVRLANNFELLFEIENTKKNEKYEILSLESNTKHGYNVAPSIFNFISNDLRTFEIKG", "aa_hexdigest": "1c48f3ad2fa9c1fc2d206b5aa3256628", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10459.763599999987, "isoelectric_point": 6.589821815490722 }, "id": "GPKLONFDEH_1045", "locus": "GCA000006625_01024" }, { "type": "cds", "sequence": "contig_1", "start": 612850, "stop": 613440, "strand": "-", "frame": 1, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274", "SO:0001217", "UniRef:UniRef50_Q9PPZ2", "UniRef:UniRef90_Q9PPZ2" ], "nt": "ATGAGTCGTTATACAGGTAGTATTTATAAAAAATCACGCCGTTTAGGATTTTCTTTATTAGAAAATAATAAAGAATTTAATTCTGGTAAAAAAAGAACATACGGTCCAGGACAACACGGTAATAAAAAAGTTAAATTATCAAATTATGGCCAACAATTAGTTGAAAAACAAAAGTTAATGTTTTTATATGGATTGAATGATCGTCAATTTAGACGTTTATATAGAGTTGCACTAGGCCGTCCAGGCGTACTTACTTTAAATTTATTACAAGTTCTTGAATCACGTTTAGATTCATTAGTTTATCGTGCTGGATTTGCTCCAACACGTCGTGCTGCACGTCAATTAGTAAATCACTCACACGTTTTGGTTAACAATAAAAAGGTTAACATCCCTTCAGCACTAGTTGAAGTAGGTAGTACAATTGCTCTTAAGGCTAAATCATTAGAAATACCTTTAATTAAAAATACTTTAAACAAACCAGCTGATTTTATTGAATTAATCGATAAAGAAAAAAAAGTTGCAAAATTAGCACGTTTACCAGAACGTAATGAATTACCAGCAGATGTAAATGAAGCTTACGTTGTTGAATGA", "aa": "MSRYTGSIYKKSRRLGFSLLENNKEFNSGKKRTYGPGQHGNKKVKLSNYGQQLVEKQKLMFLYGLNDRQFRRLYRVALGRPGVLTLNLLQVLESRLDSLVYRAGFAPTRRAARQLVNHSHVLVNNKKVNIPSALVEVGSTIALKAKSLEIPLIKNTLNKPADFIELIDKEKKVAKLARLPERNELPADVNEAYVVE", "aa_hexdigest": "d998240b216863feebdae9c3ed1f3667", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PPZ2", "query_cov": 1.0, "subject_cov": 0.9702970297029703, "identity": 1.0, "score": 375.0, "evalue": 2.61e-131, "valid": true, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_Q9PPZ2", "cog_id": "COG0522", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "GPKLONFDEH_1046", "locus": "GCA000006625_01025" }, { "type": "cds", "sequence": "contig_1", "start": 614282, "stop": 614593, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTATTCAAAGTATAATTATTTTTTAGTTCAATTAATTCATCGTATTAAAAATAAAGTTCATTTATTAAAAAATATCATTGTAGTTTTAAATTCAATCAAAATTATTGAAGAAGCTAATAAAAATAGCTTACAAAAAGTGCAAGAAATCACCTTTTTGACTGATGACAAAATTAAAGAATTAAGACAATTTAACCATGATTTTAATGATTTTATTAATAACCAATTAGCTGATTTTATTTATAATTCAAAATTTGTTAATTTAAATAACCTTTATTTAAATAGTTATTATCAACAAGCCTTAAATTGA", "aa": "MNYSKYNYFLVQLIHRIKNKVHLLKNIIVVLNSIKIIEEANKNSLQKVQEITFLTDDKIKELRQFNHDFNDFINNQLADFIYNSKFVNLNNLYLNSYYQQALN", "aa_hexdigest": "e0b1375ce7e995a377de07ae7b23b914", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12417.090599999985, "isoelectric_point": 9.126676368713383 }, "id": "GPKLONFDEH_1047", "locus": "GCA000006625_01026" }, { "type": "tRNA", "sequence": "contig_1", "start": 615445, "stop": 615520, "strand": "-", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 79.0, "nt": "GGTTTCGTAGCTCAGTCGGTAGAGCAACGGACTGAAGATCCGTGTGTCGACAGTTCGATTCTGTCCGAAACCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 615485, 615487 ], "id": "GPKLONFDEH_29", "locus": "GCA000006625_01027" }, { "type": "tRNA", "sequence": "contig_1", "start": 615522, "stop": 615597, "strand": "-", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 58.3, "nt": "GGTTCTGTGGTGTAGTGATTAACATGCCTCTCTGTCACAGAGGAGATCGCGGGTTTGACTCCCGTCAGGACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 615562, 615564 ], "id": "GPKLONFDEH_28", "locus": "GCA000006625_01028" }, { "type": "tRNA", "sequence": "contig_1", "start": 615600, "stop": 615676, "strand": "-", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 71.8, "nt": "CGCGGGATAGAGCAGCTTGGTAGCTCGTCAGGCTCATAATCTGGAGGTCGGGGGTTCAAATCCCTCTCCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "GPKLONFDEH_27", "locus": "GCA000006625_01029" }, { "type": "tRNA", "sequence": "contig_1", "start": 615734, "stop": 615826, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 81.0, "nt": "GGAGAAATACCCAAGTCCGGCTGAAGGGATCAGTCTTGAAAACTGAGAGGCGATGCAAGTCGCGCGGGGGTTCGAATCCCTCTTTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 615803, 615805 ], "id": "GPKLONFDEH_26", "locus": "GCA000006625_01030" }, { "type": "tRNA", "sequence": "contig_1", "start": 615895, "stop": 615972, "strand": "-", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 72.5, "nt": "GCTTCTATAGCTCAATTTGGTTAGAGCCCCCGACTCATAATCGGTTGGTTACAGGTTCAAGTCCTGTTAGAAGCACCA", "db_xrefs": [ "SO:0000263" ], "id": "GPKLONFDEH_25", "locus": "GCA000006625_01031" }, { "type": "tRNA", "sequence": "contig_1", "start": 615975, "stop": 616051, "strand": "-", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 75.1, "nt": "GGCTGGATAGCTCAACTGGTTAGAGCGCTCGGTTCATACCCGGGAGGTTCAGAGTTCAAATCTCTGTCCAGCCACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 616015, 616017 ], "id": "GPKLONFDEH_24", "locus": "GCA000006625_01032" }, { "type": "tRNA", "sequence": "contig_1", "start": 616056, "stop": 616132, "strand": "-", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 83.2, "nt": "CGGGAAGTAGCACAGCTTGGTAGTGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 616113, 616115 ], "id": "GPKLONFDEH_23", "locus": "GCA000006625_01033" }, { "type": "tRNA", "sequence": "contig_1", "start": 616180, "stop": 616256, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(acg)", "amino_acid": "Arg", "anti_codon": "acg", "score": 74.1, "nt": "GCATCCTTAGCTCAATTGGACAGAGCGTCTGGCTACGGACCAGAAGGTTAAGGGTTCGACTCCTTTAGGGTGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 616220, 616222 ], "id": "GPKLONFDEH_22", "locus": "GCA000006625_01034" }, { "type": "tRNA", "sequence": "contig_1", "start": 616260, "stop": 616334, "strand": "-", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 62.3, "nt": "GGTGCCATAGCCAAGTGGTAAGGTCAGGAGCTGCAACCTCCTTATTCGTCAGTTCGAATCTGACTGGCACCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 616300, 616302 ], "id": "GPKLONFDEH_21", "locus": "GCA000006625_01035" }, { "type": "cds", "sequence": "contig_1", "start": 616455, "stop": 616910, "strand": "-", "frame": 2, "gene": "ymdB", "product": "Metallophosphoesterase", "db_xrefs": [ "COG:COG1692", "COG:T", "EC:3.1.4.16", "KEGG:K09769", "SO:0001217", "UniRef:UniRef50_A0A2C9DYY2", "UniRef:UniRef90_A0A2C9DYY2" ], "nt": "ATGATTCGTGTAACTAATTTATTAGGTAATTCTATTGATATGAAAGGTTTACAAACAAATCCTTTTGAATCTTTAGATAAAATTATTGCTTTTAATGAAGCACCGATTCATATTGTTGATTTTCATGCTGAAACAACAAGTGAAAAAAATGCACTTTTTTTAGATTTTAAAAGTAAATTGTCTTTAGTTTATGGTACTCATACACATATTCCAACAAATGATGCTAGAATTGTCGATCATACGGCTTTTCAAACTGATTTAGGAATGTGTGGTGCTGCTAATAGCGTGATTGGGGCAAATCCAAGTAGTATTATTCAACGATTTCGTGATCCAAATAAACGTTTTATATTAGAACCAAGTGATGAAAAATATGTTTTTTGTGCTAGTTTAGTTGAAATTGATGATGAAACAAATTTTCCAACTAATATTACACCTATTGTAATTTATGAAAAATAA", "aa": "MIRVTNLLGNSIDMKGLQTNPFESLDKIIAFNEAPIHIVDFHAETTSEKNALFLDFKSKLSLVYGTHTHIPTNDARIVDHTAFQTDLGMCGAANSVIGANPSSIIQRFRDPNKRFILEPSDEKYVFCASLVEIDDETNFPTNITPIVIYEK", "aa_hexdigest": "da17ba31d4e02903caca940f7409a080", "start_type": "ATG", "rbs_motif": null, "genes": [ "ymdB" ], "truncated": "3-prime", "pseudogene": { "start": 616455, "stop": 617210, "observations": { "mutation": [ 617039, 617102 ], "stop": [ 617039, 617102 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYY2", "query_cov": 1.0, "subject_cov": 0.5741444866920152, "identity": 0.96, "score": 291.0, "evalue": 7.97e-98, "gene_start": 1, "gene_end": 151, "reference_start": 113, "reference_end": 263, "reference_sequence": "MKILAIGDIFSKQGRKAVANELPKLKQEHQIDFVIANAENTTHGRGLCWKHYEELVGYGIDCITMGNHTWDHPDIFEILTTKTNIIRPYNIINTHQYHLVGSGSRVFYCNKKMIRVTNLLGNSIDMKGLQTNPFESLDKIIACNEAPIHIVDFHAETTSEKNALFLDFKGKLSLVYGTHTHIPTNDARIVDRTAFQTDLGMCGAANSVIGANPSSIIQRFRDPNKRFILEPSDEKYVFCASLVEIDDETNQPIKITPIVIYEK" }, "paralog": false, "identity": 0.9601593625498008, "score": 489.0, "evalue": 1.11e-178, "description": "Internal stop codon at 617039, 617102. Nonsense mutation around 617039, 617102.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYY2", "gene": "YmdB", "product": "Metallophosphoesterase", "ec_ids": [ "3.1.4.16" ], "uniref50_id": "UniRef50_A0A2C9DYY2", "cog_id": "COG1692", "cog_category": "T", "kegg_orthology_id": "K09769", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYY2", "EC:3.1.4.16", "UniRef:UniRef50_A0A2C9DYY2", "COG:COG1692", "COG:T", "KEGG:K09769" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYY2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYY2" ], "product": "Metallophosphoesterase" } }, "id": "GPKLONFDEH_1048", "locus": "GCA000006625_01036" }, { "type": "cds", "sequence": "contig_1", "start": 617100, "stop": 617246, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13277.11" ], "nt": "ATGAAAATTTTAGCTATTGGAGATATTTTTTCAAAACAAGGAAGAAAAGCAGTGGCCAATGAATTGCCCAAGCTAAAACAAGAGTATCAAATTGATTTTGTAATTGCTAATGCTGAGAATACAACACATGGTCGCGGTTTGTGTTGA", "aa": "MKILAIGDIFSKQGRKAVANELPKLKQEYQIDFVIANAENTTHGRGLC", "aa_hexdigest": "9b76044b46dbd0a4ca3561f7101ebe28", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13277.11", "name": "YmdB", "length": 44, "aa_cov": 0.9166666666666666, "hmm_cov": 1.0, "evalue": 1.1405217051240912e-16, "score": 56.39116668701172, "start": 4, "stop": 47 } ], "seq_stats": { "molecular_weight": 5331.134800000001, "isoelectric_point": 8.978850364685059 }, "id": "GPKLONFDEH_1049", "locus": "GCA000006625_01037" }, { "type": "cds", "sequence": "contig_1", "start": 617250, "stop": 618440, "strand": "-", "frame": 2, "gene": "uvrD", "product": "DNA 3'-5' helicase", "db_xrefs": [ "COG:COG0210", "COG:L", "SO:0001217", "UniRef:UniRef50_Q9PPY9", "UniRef:UniRef90_Q9PPY9" ], "nt": "TTGTTAGAAGAAGGAACGCCAGCTAACCAAATCGCTGTTTTGTTCCGAAGCAATCACTATTCTCGTACGATTGAACAATCAATGATGCGCGAGAGCATTCCTTATACAATTCTTGGCAGTAAAAAGTTTTATGAACGTGCTGAAATTAAGGACATGATTGCTTATTTAAAAGTAGTTAATGATTTAGATGAACTATCTTTTTTAAGGATTATTAATACACCACGTCGTGCGATTGGTCCAACTACTTTTGAACATGTTAAACATTATGCAATAAATAATAATTTATTTTTATTTGAAGCATTAGCTGAAGTTGAAAAAAACCATTTAATAAATAACACACAAAAAAAGAATATTTTAAATTTTGTTAATTTAATTAAAGAAATCCGTGATGAAATGGAAGATTTAAAAATTCATGAGATTTTAGAATTAATTTACAAAAAAGTTAACTATGAAGCTTATTTATTAGAAAACGAAAAAGCAGAAGATAAAATCGATAATGTTTATGAATTAAAACGCGCTATGAAAATGTATGTTGATCGTCATCCAGATGACACGATTAATGATTATTTAAATTCGATTGCTTTATATTTGAATAAGGATGGTAAAGATAGTAAAGAAAATGTTTTATTAATGACAGTTCATAATTCTAAAGGTCTAGAATATGAAAACGTTTTCGTTGCTGGTATGAACGAAGGACTTCTTCCTTCTGATCGAGCAATTAATGATGATCCAATTAAAGGAGTTGAAGAAGAACGAAGAATTGCTTATGTAGCCTTAACACGAGCAAAAAAAAATCTTTATATTTCATCTGCTTGTTGTTATGATCCATTAGCAAGAAGACAAGTATTTGAAAGTCGTTTTATTAATGAAATTGGTTTTAATAATTTAAAGATTATAAATAGTAGTTTTAAAAATAATAAACCAGAGGATATGCCATTAAAATCATTCTTAAAACAACAAGAAGAACGTTCATGGTTCGATTCAAAACAAAAACAAAAAGAAGTTGAAGATAATTTTTATCAAACAATGAAAAACGATTTTGAAATTGGTGAAAGAATTGTTCACACAAGTTTTGGTGATGGTGTTATTATTGGAATTGATGGTGATATCTTAACAATTGCTTTCAGTAAATATCGTCAACCAAAACAAATTAATTTTAATCATAAAACAATCGTAAGAAGAATAGGTTAA", "aa": "MLEEGTPANQIAVLFRSNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMIAYLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMEDLKIHEILELIYKKVNYEAYLLENEKAEDKIDNVYELKRAMKMYVDRHPDDTINDYLNSIALYLNKDGKDSKENVLLMTVHNSKGLEYENVFVAGMNEGLLPSDRAINDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFNNLKIINSSFKNNKPEDMPLKSFLKQQEERSWFDSKQKQKEVEDNFYQTMKNDFEIGERIVHTSFGDGVIIGIDGDILTIAFSKYRQPKQINFNHKTIVRRIG", "aa_hexdigest": "258d64f50209ebbddeac128b4c1a4cb3", "start_type": "TTG", "rbs_motif": null, "genes": [ "uvrD" ], "truncated": "3-prime", "pseudogene": { "start": 617250, "stop": 618740, "observations": { "mutation": [ 618464, 618677 ], "stop": [ 618464, 618677 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPY9", "query_cov": 1.0, "subject_cov": 0.5329744279946165, "identity": 0.9570000000000001, "score": 744.0, "evalue": 2.95e-265, "gene_start": 1, "gene_end": 396, "reference_start": 348, "reference_end": 743, "reference_sequence": "MGVGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAHLITEKHIHPSRILGFTFTNKAADEMKERVGKTIGVSIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDADDQEVLLKEIFDQLSIEKKSPIIKKTIKMISKVKNKFFDQSDIMNEKNRKYLEIVDRNDAQKLADIYTIYCDRCFKLNYVDFDDLINLTYKLFAEFPEVLEKWQNKFDYILVDEFQDTNKIQYDLISLLAAKHQNLFVVGDPDQMIYSFRGAEQWIINNFNQNFKNTKVTILKTNYRSTQPILDTANRLIDANNNSYKKNLTAFNTNDHNIPIYLRGQNQIDEANWIARTVKELLEEGTPANQIAILFRSNHYSRTIEQSMMRESIPYTILGSKKFYERSEIKDMIAYLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMEDLKLHEVLELIYKKVNYEAYLLENERAEDKIDNVSELKRAMKMYVDRHPDDTINDYLNSIALYLNKDSKDSKENVLLMTVHNSKGLEYENVFVVGMNEGHLPSDRAIDDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFNNLKIINSSFKNNKPEDMSLKSFLKQQEERSWFDSQQKQKEAEDNFYQTMKNDFEIGERIVHTSFGNGVIIGIDGDILTIAFSKYRQPKQISFNHKTIVRRMG" }, "paralog": false, "identity": 0.9455645161290323, "score": 941.0, "evalue": 0.0, "description": "Internal stop codon at 618464, 618677. Nonsense mutation around 618464, 618677.", "psc": { "uniref90_id": "UniRef90_Q9PPY9", "gene": "UvrD", "product": "DNA 3'-5' helicase", "uniref50_id": "UniRef50_Q9PPY9", "cog_id": "COG0210", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPY9", "UniRef:UniRef50_Q9PPY9", "COG:COG0210", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPY9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPY9" ], "product": "DNA 3'-5' helicase" } }, "id": "GPKLONFDEH_1050", "locus": "GCA000006625_01038" }, { "type": "cds", "sequence": "contig_1", "start": 618831, "stop": 619481, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF13604.12", "PFAM:PF00580.27" ], "nt": "ATGGGATTAGGAATTGATTATTCACGCTTAAATCAAGAACAAAAAGAAGCAGTTACAGCAGACTTAGGACCGCAATTAGTAATTGCAGGGGCAGGGACAGGAAAAACATCTGTTTTAACTTTACGTATTGCTTATTTAATTACTGAAAAAAATATTCATCCATCACGGATTTTAGGTTTTACTTTTACTAATAAGGCTGCTGATGAAATGAAAGAACGTGTTGGAAAAACAATTGGCGTTTCTATTCCTTATTTAAGTACTTTTCATTCAATGTGTGTAAAAATTTTACAACAAGATATTCATTATTTAAATTATCATAACAATATTAAAATTATTGATACAGACGATCAAGAAGTTTTGCTTAAAGAAATTTTTGATCAATTAAACATTGAAAAAAAATCGCAAGTTATTAAGAAAATAATTAAAACGATTAGTAAAGTTAAAAATAAATTTTTTGATCAAAACGATATGCTAAATGAAAAAAATCACAAGTATTTAGAATTGGTTGATTTAAATGATGCACAAAGATTAGTTGATATTTATAAGATTTATTGTGATCGATGTTTTAAATTAAATTATGTTGATTTTGATGATTTGATCAATTTAACTCATAAGTTATTTATTGAGTTTCCAGAAGTTTTAGAAAAATGA", "aa": "MGLGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAYLITEKNIHPSRILGFTFTNKAADEMKERVGKTIGVSIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDTDDQEVLLKEIFDQLNIEKKSQVIKKIIKTISKVKNKFFDQNDMLNEKNHKYLELVDLNDAQRLVDIYKIYCDRCFKLNYVDFDDLINLTHKLFIEFPEVLEK", "aa_hexdigest": "509d217b6969f29a2540259fa29517d7", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 158, "aa_cov": 0.6990740740740741, "hmm_cov": 0.9177215189873418, "evalue": 1.6942742227932514e-09, "score": 33.40038299560547, "start": 10, "stop": 160 }, { "id": "PF13245.12", "name": "AAA_19", "length": 104, "aa_cov": 0.4722222222222222, "hmm_cov": 0.7211538461538461, "evalue": 1.8428264881415213e-15, "score": 52.87610626220703, "start": 14, "stop": 115 }, { "id": "PF13604.12", "name": "AAA_30", "length": 70, "aa_cov": 0.3055555555555556, "hmm_cov": 0.9428571428571428, "evalue": 1.8927886582341096e-08, "score": 29.856014251708984, "start": 10, "stop": 75 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 213, "aa_cov": 0.9537037037037037, "hmm_cov": 0.971830985915493, "evalue": 1.9929625561797648e-53, "score": 177.3812255859375, "start": 10, "stop": 215 } ], "seq_stats": { "molecular_weight": 25001.65500000004, "isoelectric_point": 6.597154045104981 }, "id": "GPKLONFDEH_1051", "locus": "GCA000006625_01039" }, { "type": "cds", "sequence": "contig_1", "start": 619506, "stop": 619907, "strand": "-", "frame": 2, "gene": null, "product": "RDD family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Z9", "UniRef:UniRef90_A0AAC9X6Z9" ], "nt": "ATGGGATTTTGTTTTGTTTTTGCTGATGCAAGTTTTAAATCAATTCAAGCTATGAATGAGACTGAGCTAAAAGAGTATTATCATCAAATTAAAGATTTAAAAGCTTATGTGCAGTCAATTTTATCTTTAAAAATTAATGATAATTTCACCAATACTACAACTATGGCTATTTTAAGTATTTTAGTTAAAAGTCTATATTCAATTATGGGTTTAGTTAGTTGTATTATTGTAATTCACATGTTTATGAATTCAAAAAAACGTGCTTTACATGATCAAGTAGCAAAAGTTGTTTTATTATGGATGCATTATGAGAAACCAAAACAAGCGCCTAAACATAAAACAAAACGTGAAGTTGAGATTCAAAAAATCGATAATTTAATTCAAGAACTAGATAAAATATAA", "aa": "MGFCFVFADASFKSIQAMNETELKEYYHQIKDLKAYVQSILSLKINDNFTNTTTMAILSILVKSLYSIMGLVSCIIVIHMFMNSKKRALHDQVAKVVLLWMHYEKPKQAPKHKTKREVEIQKIDNLIQELDKI", "aa_hexdigest": "759059ab575e854e489d5f5dcfb839ac", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 619506, "stop": 620207, "observations": { "mutation": [ 619940, 620030, 620126 ], "stop": [ 619940, 620030, 620126 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6Z9", "query_cov": 1.0, "subject_cov": 0.48717948717948717, "identity": 0.94, "score": 239.0, "evalue": 1.24e-77, "gene_start": 1, "gene_end": 133, "reference_start": 141, "reference_end": 273, "reference_sequence": "MSDKTTLKQENTIEYPVVSALRRAGAGVLDYIFVSILAFLLSLLVFINSNFKISDVFVNNDELAPEPWRLFLMAIIAFFVYCTYYIIIPVFCKSYTLFRWAFSIRMISLVKQNDKKILFVALIKHSLLTWLIFIMINLLAMGFCFAFADASFKSIEAMNEIELKEHYRQIKDLKAYVQSILSLKINDNFTSTTTMAILSILVKSLYSIMGLISCIIVIHMFMNSKKRTLHDQVAKVVLLWMHYEKPKQAPKHKTKREVEIQKIDNLIQELDKI" }, "paralog": false, "identity": 0.9227467811158798, "score": 420.0, "evalue": 1.98e-151, "description": "Internal stop codon at 619940, 620030, 620126. Nonsense mutation around 619940, 620030, 620126.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6Z9", "product": "RDD family protein", "uniref50_id": "UniRef50_A0AAC9X6Z9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6Z9", "UniRef:UniRef50_A0AAC9X6Z9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6Z9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6Z9" ], "product": "RDD family protein" } }, "id": "GPKLONFDEH_1052", "locus": "GCA000006625_01040" }, { "type": "cds", "sequence": "contig_1", "start": 620124, "stop": 620327, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGATAAGTCAACATTAAAACAAGAAAATACGATCGAATATCCCGTTGTTGGTGCACTAAGGCGTGCTAGCGCTAGTGTTTTAGATTATATTTTTATTTCCATTATTGCTTTTTTATTATCTTTATTAGTTTTTATTAATAGTAATTTTAAAATTAGTGATATTTTTGTTAATAATGATGAGCTTGCTCCTGAACCTTGA", "aa": "MSDKSTLKQENTIEYPVVGALRRASASVLDYIFISIIAFLLSLLVFINSNFKISDIFVNNDELAPEP", "aa_hexdigest": "e79cb96ff433d6ab6caca331ea5a8973", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7492.551999999994, "isoelectric_point": 4.507070732116699 }, "id": "GPKLONFDEH_1053", "locus": "GCA000006625_01041" }, { "type": "cds", "sequence": "contig_1", "start": 620328, "stop": 620534, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAAATTTATGAATTATACACAATTAATATTAATTCTAAAAAACAAATTAAAGAACGAACAATGATTATTAGTCATGCCTTATCAAATGCTAAAAAGTATTTAGAGATTTATTTTGCTAATTTTGAGAATAATTTATATGTTCGCAAGTTTAAAATTAATATTTTAAAATATTATTTAAAATCAAAACAGGGATTAAATTAA", "aa": "MKKIYELYTININSKKQIKERTMIISHALSNAKKYLEIYFANFENNLYVRKFKINILKYYLKSKQGLN", "aa_hexdigest": "c1ca994b2b1a2a3d7078282e7589c90d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8267.709199999996, "isoelectric_point": 9.952709770202638 }, "id": "GPKLONFDEH_1054", "locus": "GCA000006625_01042" }, { "type": "cds", "sequence": "contig_1", "start": 620997, "stop": 621266, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00535.33" ], "nt": "ATGTTATTAACAATTGCTTTTTATTTATCAAAGACAACTCATTATCTAAAAAAAAATTTTAATTATTTTTTAGATTTATTAAATCAAAATCAAAAAAATATTGAATTAATTATTATTGATGATGCTAGTGATTATAATTTATTTAAAACATTAAAACCATTAATTGAAAATACTAATGCTAAGATTAAATATTTTTATTTAAATGAAACACAAGGCAATGCTTATGCATATAATTTAGCAACGAAATATGCTCACGGTAAATACATTTGA", "aa": "MLLTIAFYLSKTTHYLKKNFNYFLDLLNQNQKNIELIIIDDASDYNLFKTLKPLIENTNAKIKYFYLNETQGNAYAYNLATKYAHGKYI", "aa_hexdigest": "f0d60c60e7711154fda364d3fcc711b8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00535.33", "name": "Glycos_transf_2", "length": 65, "aa_cov": 0.7303370786516854, "hmm_cov": 0.9846153846153847, "evalue": 2.1258417133125918e-11, "score": 39.6137580871582, "start": 25, "stop": 89 } ], "seq_stats": { "molecular_weight": 10526.976299999991, "isoelectric_point": 9.048347282409669 }, "id": "GPKLONFDEH_1055", "locus": "GCA000006625_01043" }, { "type": "tRNA", "sequence": "contig_1", "start": 621353, "stop": 621429, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 77.8, "nt": "GCACCCATAGTTCAATTGGTTAGAATATATGCCTTCGAAGCATAGGGTTAGGAGTTCGAGTCTCTTTGGGTGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 621393, 621395 ], "id": "GPKLONFDEH_20", "locus": "GCA000006625_01044" }, { "type": "tRNA", "sequence": "contig_1", "start": 621507, "stop": 621583, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 78.7, "nt": "GCACCTGTAGCTCAGTTGGATAGAGCATACGCCTTCTAAGCGTACGGTCTGGGGTTCGAATCCCTTCAGGTGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 621547, 621549 ], "id": "GPKLONFDEH_19", "locus": "GCA000006625_01045" }, { "type": "cds", "sequence": "contig_1", "start": 621874, "stop": 622476, "strand": "+", "frame": 1, "gene": null, "product": "DUF1410 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0I9", "UniRef:UniRef90_A0AAC9T0I9" ], "nt": "ATGATCAATAATTCACCAAACATTAATTATTATTTACAAGATAAAAAATATATTCCAAAATATGCTTACTATGTTAATAAAAAACAAAAACCTATCGAGCTATTTGCACGATATAAAGGACGTCCTGCTCTTTTTATTCGTGAAAATACCATGATTAATACTTCATTAAATTCAAATGTATTAAATTTAGACTTTGAATTTGAAGTTGCAGGAGATTTTAAATTACCTATTAATTACTTTTTTATGGATATAAAAAGTGATATAGGTGAATTTCGTAATTTAGAAGTTTATGAAACTAAACACTCTTTGTATGAAATCGGAGGAGTGATTTATGACTTATACACTTATAAAACCAATTTAAAAATTAATCTTAATCAAGCTGGTTTTGTTAATAAGAACACTTTAAGTAAAAAAATTAATTTTGCTTTTTTTCTTCGTATTAATGATGATAAACGAATTCATGAACAAATTAATTATACATGTGAAAAAAGTTTGTTTTTTGAGGCAGTACAAAAAGAATTTCAAGATCTTAATTTTGATTATTTTTACAAAAATACATTTTTATTAAATAAACACGAAAAAGTTAATCCTACTAATTTTTGA", "aa": "MINNSPNINYYLQDKKYIPKYAYYVNKKQKPIELFARYKGRPALFIRENTMINTSLNSNVLNLDFEFEVAGDFKLPINYFFMDIKSDIGEFRNLEVYETKHSLYEIGGVIYDLYTYKTNLKINLNQAGFVNKNTLSKKINFAFFLRINDDKRIHEQINYTCEKSLFFEAVQKEFQDLNFDYFYKNTFLLNKHEKVNPTNF", "aa_hexdigest": "b498cecc261f1848b2caad9cb80f8518", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 621853, "stop": 622779, "observations": { "mutation": [ 622474, 622495, 622591, 622681 ], "stop": [ 622474, 622495, 622591, 622681 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0I9", "query_cov": 1.0, "subject_cov": 0.5167958656330749, "identity": 1.0, "score": 403.0, "evalue": 1.53e-139, "gene_start": 1, "gene_end": 200, "reference_start": 8, "reference_end": 207, "reference_sequence": "MKKKVIKMINNSPNINYYLQDKKYIPKYAYYVNKKQKPIELFARYKGRPALFIRENTMINTSLNSNVLNLDFEFEVAGDFKLPINYFFMDIKSDIGEFRNLEVYETKHSLYEIGGVIYDLYTYKTNLKINLNQAGFVNKNTLSKKINFAFFLRINDDKRIHEQINYTCEKSLFFEAVQKEFQDLNFDYFYKNTFLLNKHEKVNPTNFWETIPLFWIQDKELDNEIKIKNNKRYIFNTKFSLNDDNSWNNRPTKETFELIKYSLEYVNLAKENKKIEWIEDYQNINITYDYYQRPIDFLLKMKTKFLYDFKNDQLSINNNYGFNGIWLPNKQIAKLKITVINNYLTPQKFVINQLINIENDFNSINKQKISVQKAIIENYENFKSLEN" }, "paralog": false, "identity": 0.987012987012987, "score": 615.0, "evalue": 1.64e-225, "description": "Internal stop codon at 622474, 622495, 622591, 622681. Nonsense mutation around 622474, 622495, 622591, 622681.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0I9", "product": "DUF1410 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9T0I9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0I9", "UniRef:UniRef50_A0AAC9T0I9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0I9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0I9" ], "product": "DUF1410 domain-containing protein" } }, "id": "GPKLONFDEH_1056", "locus": "GCA000006625_01046" }, { "type": "cds", "sequence": "contig_1", "start": 622988, "stop": 623779, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY82", "UniRef:UniRef90_A0A2C9DY82" ], "nt": "ATGAAAATTTTAAATCACTTGAAAATTAATTGGCGTAGTCATAAAATAAAGGTGATTGTTGGTGGAATTATTAGTTTATTAATTGTAATTACTGCTATTATTTTAGGTGTTATTCTATCAACTCAAAATACAAATAAAATTAGTAAAAAAGAACCCTTAACTCAAATTGAAGATAATATTAATAATAAAAAACAAGATGCACATAAGGTTAATGAAAATATAACAATTATTGATAAAAAAGGTGGTATAATCCATAAAAAGACAGACATTACAAAAATTAACCAAATAAATCAACAAAAAGAAATTTTAAAGGATTATTTAAAAAATAAAGAAGAAAAAAATAAGGATCAAATAAATAATACTAACGAAAATCTAAATAAACCAATAATTAATGTTAAAAATGTAGATGATAAAAACAAACAAGAAAATTCAACTAAATTAAAAAATAATGATTTTATTTCTAATAATGATAAAAACAATAAAATCAACGAAAATAATAATATAAGTTATGAAGAAAAACCTTTTAAACTAAAACGCTTCAATATTATTAATGTAATTGCGAAAAATAAAGATTTATACCAATTATTAGATTTCAAAACTTATTTTTCTAATGAAAACATTAATAATGATATTAAATTTAATGAAAAGTTGTTTATTAAAAATATCTATGAAATAGTCAAATCTGCTATAAGTAGTTTTCAAGAATTTTGTAATATCATGCAGTATATTAAAATTGATATCAAATATAAATTTAATAAAGATGCAAAAAACATAATTGTTATTGCTAATTGA", "aa": "MKILNHLKINWRSHKIKVIVGGIISLLIVITAIILGVILSTQNTNKISKKEPLTQIEDNINNKKQDAHKVNENITIIDKKGGIIHKKTDITKINQINQQKEILKDYLKNKEEKNKDQINNTNENLNKPIINVKNVDDKNKQENSTKLKNNDFISNNDKNNKINENNNISYEEKPFKLKRFNIINVIAKNKDLYQLLDFKTYFSNENINNDIKFNEKLFIKNIYEIVKSAISSFQEFCNIMQYIKIDIKYKFNKDAKNIIVIAN", "aa_hexdigest": "7ca3f3936526376426828e90952f11d4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2C9DY82", "query_cov": 1.0, "subject_cov": 0.9195804195804196, "identity": 1.0, "score": 444.0, "evalue": 2.93e-156, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A2C9DY82" }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DY82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DY82" ], "product": "Lipoprotein" }, "genes": [], "id": "GPKLONFDEH_1057", "locus": "GCA000006625_01047" }, { "type": "cds", "sequence": "contig_1", "start": 623865, "stop": 624098, "strand": "+", "frame": 3, "gene": "acpP", "product": "Acyl carrier -like protein", "db_xrefs": [ "COG:COG0236", "COG:I", "GO:0006633", "RefSeq:WP_006688580.1", "SO:0001217", "UniParc:UPI000012531D", "UniRef:UniRef100_Q9PPY4", "UniRef:UniRef50_Q9PPY4", "UniRef:UniRef90_Q9PPY4" ], "nt": "ATGTCTATTAATATTAAAGATTTAATCATGAAAATCGCTAAGGAAAATAAAATTGCTTTAAATATGGATAATTTAAATATCGAATTAAAATCATTAGGAATTGATTCGCTAAGTGCTATGAATTTAATAATGAAAATTGAGGATCAAATTGGCGTTCAATTGCCTGATGAAAAATTATTAAAAATCAAAAATTTACGTGATTTAATTAACGCGTTTGAAGATGTATTAAAATAA", "aa": "MSINIKDLIMKIAKENKIALNMDNLNIELKSLGIDSLSAMNLIMKIEDQIGVQLPDEKLLKIKNLRDLINAFEDVLK", "aa_hexdigest": "3679fe4270a1d663e5c2b0ff64e2bb56", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012531D", "ncbi_nrp_id": "WP_006688580.1", "uniref100_id": "UniRef100_Q9PPY4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012531D", "RefSeq:WP_006688580.1", "UniRef:UniRef100_Q9PPY4" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPY4", "uniref90_id": "UniRef90_Q9PPY4" }, "psc": { "uniref90_id": "UniRef90_Q9PPY4", "gene": "AcpP", "product": "Acyl carrier protein homolog", "uniref50_id": "UniRef50_Q9PPY4", "cog_id": "COG0236", "cog_category": "I", "go_ids": [ "GO:0006633" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPY4" ], "product": "Acyl carrier protein homolog" }, "genes": [ "acpP" ], "id": "GPKLONFDEH_1058", "locus": "GCA000006625_01048" }, { "type": "tRNA", "sequence": "contig_1", "start": 624162, "stop": 624236, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 73.7, "nt": "AGGGGTATAGTTCAATGGTAGAACTGCGGACTTCAAATCCGCGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCA", "db_xrefs": [], "id": "GPKLONFDEH_10", "locus": "GCA000006625_01049" }, { "type": "cds", "sequence": "contig_1", "start": 625203, "stop": 625412, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGTTAAATTCAAATGTTGCTGATATTGCTAACTCAGTATCTGATCCTCGTGGTGGTTCTTCACGTGCAGCATGTTTCTTAAAAGAATTTACTGAAGGCGTTCCATATGCTCATTTTGACATTGCTATTACTGCAGATGTTGGTCATAAAGGAACTGGTGTAATGTTAAGAACATTTTATCGTATTGCTCAAAATCAAAAATTTAATTAG", "aa": "MLNSNVADIANSVSDPRGGSSRAACFLKEFTEGVPYAHFDIAITADVGHKGTGVMLRTFYRIAQNQKFN", "aa_hexdigest": "33932f43f0753e41d32c6bc2e60dcd24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 66, "aa_cov": 0.8405797101449275, "hmm_cov": 0.9848484848484849, "evalue": 3.5184007966262606e-13, "score": 45.05320358276367, "start": 2, "stop": 59 } ], "seq_stats": { "molecular_weight": 7507.374599999999, "isoelectric_point": 7.944197273254395 }, "id": "GPKLONFDEH_1059", "locus": "GCA000006625_01050" }, { "type": "cds", "sequence": "contig_1", "start": 625509, "stop": 625832, "strand": "-", "frame": 2, "gene": null, "product": "Cytosol aminopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5A7N3P8" ], "nt": "ATGAAATTTGATATGTCTGGTTCTGCTATTGTAAGTATGACAGTGAGAGCTTTAGCTTTAAATAAAGAAAAAGTTAATGTTGTGGCTGTATGTCCATTAGTATTAAATTTATTAGCTCCAGAAGGTCAAAAACCAGATGATATAGTGAAATCATATAATGGTAAAACAATTGAATTAGATAACACTGATGCAGAAGGACGTTTAATTTTAGCAGATGCACTAACATATGCTGTTCGTGATTTAAAAGCTTCTAAATTATTTGATGTTGCAACTTTAACAGGTGCTATGATTTATGCTTTAGGTGATACATATTCTGGTGTATGA", "aa": "MKFDMSGSAIVSMTVRALALNKEKVNVVAVCPLVLNLLAPEGQKPDDIVKSYNGKTIELDNTDAEGRLILADALTYAVRDLKASKLFDVATLTGAMIYALGDTYSGV", "aa_hexdigest": "3c31fce772513910ea5efcc358a72d89", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A5A7N3P8", "query_cov": 0.8504672897196262, "subject_cov": 0.8348623853211009, "identity": 0.516, "score": 85.5, "evalue": 1.61e-19, "valid": false, "product": "Cytosol aminopeptidase domain-containing protein", "uniref50_id": "UniRef50_A0A5A7N3P8" }, "pscc": { "uniref50_id": "UniRef50_A0A5A7N3P8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5A7N3P8" ], "product": "Cytosol aminopeptidase domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_1060", "locus": "GCA000006625_01051" }, { "type": "cds", "sequence": "contig_1", "start": 625833, "stop": 626570, "strand": "-", "frame": 2, "gene": "pepB", "product": "leucyl aminopeptidase", "db_xrefs": [ "COG:COG0260", "COG:E", "EC:3.4.11.1", "SO:0001217", "UniRef:UniRef50_A0AAP9ACA2", "UniRef:UniRef90_A0AAP9ACA2" ], "nt": "ATGACATTAAATGAAAAAAAAGAATTTGTTTATGAATTAAAAGCTGTTGAAAAAAATCACAACCTAAAAGGTTTTGAAGAAAATACAACTGAACATGTTGTTTTTGGAGGTTTATATCAAAAACAACAATACTTAATTTTAACACCAGATTTTGATTCTAATGAACTATCAAGATCATTGATTAGTTTTTTAGAAAAATCACCTAAATCTGTTAGTGTTGATTTAAATTCATTTCTATCACTTGTCCCAGAATCAAGACATGCATCTTTATTAAATGTTGTTATTAGTGCATTAGAATATGTTGAAGTAACGCCATTTTCACTAAAATCTAAAATTGAAACAAAAAATATACATAATTTAGTAGTTGATCAAAAATATCATGATTTAATTAATAAATTACATGTAATTGCTCAATCACAAACAATTACAAGAACATTACAAGATACGCCAGCTAATTTAATGACACCAGGTGATTTTGAAGAACGAATTAAAGAATTATTTAAAGACTTGCCAGAAGTTAAAGTAAGTGTTTTATATCGTAAAGATTTAGAAGCTAAAGGAATGAACGCTCATGTTGGTGTTGGTAAAGCTGCAGTAAGTGATAAAGCACAGCCTCGTTTAGTTGTTGTTGAATATAATAATAATCCAGAAACAAATGAAAAATATGCTTTTGTTGGTAAGGGTGTATGTTTTGATTCAGGTGGATATAATGTAAAAACGGGATCACATATGCGTTGA", "aa": "MTLNEKKEFVYELKAVEKNHNLKGFEENTTEHVVFGGLYQKQQYLILTPDFDSNELSRSLISFLEKSPKSVSVDLNSFLSLVPESRHASLLNVVISALEYVEVTPFSLKSKIETKNIHNLVVDQKYHDLINKLHVIAQSQTITRTLQDTPANLMTPGDFEERIKELFKDLPEVKVSVLYRKDLEAKGMNAHVGVGKAAVSDKAQPRLVVVEYNNNPETNEKYAFVGKGVCFDSGGYNVKTGSHMR", "aa_hexdigest": "88ef1d847b061d2a66aed0354a6aaf2b", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "pepB" ], "truncated": "5-prime", "pseudogene": { "start": 625530, "stop": 626570, "observations": { "mutation": [ 625835 ], "stop": [ 625835 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAP9ACA2", "query_cov": 1.0, "subject_cov": 0.5384615384615384, "identity": 0.927, "score": 449.0, "evalue": 7.84e-156, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 245, "reference_sequence": "MTLNEKKEFVYELKAVKKDHNLKGFEKNTAEHVVFGEVYQKQHYLILTPDFDSNELSRSLVSFLEKSPKPVSVDLNSFLDLVPESRHASLLNVVVSALEYVEVTPFSLKSKVEPKNIHNLVVDAKYHDLIAKLQVIAQSQTITRTLQDTPANLMTPGDFEERIKELFKDLPEVKVSVLYRKDLEAKGMNAHVGVGKAAVSDKAQPRLVVVEYNNNPDTDEKYAFVGKGVCFDSGGYNVKTGSHMRWMKFDMSGSAIVSMTVRALALNKEKVNVVAVCPLVLNLLAPEGQKPDDIIKSYNGKTIELDNTDAEGRLILADALTYAVRDLKASKLFDIATLTGAMIFALGDTYSGVWATNNDIWNEVVVAADYAGELVWRLPFHNDFLKMLNSNVADIANSVTDPRGGSSRAACFLKEFTEGVPYAHFDIAITADVGHKGTGVMLRTFYRIAQNQKFN" }, "paralog": false, "identity": 0.9364161849710982, "score": 638.0, "evalue": 8.47e-233, "description": "Internal stop codon at 625835. Nonsense mutation around 625835.", "psc": { "uniref90_id": "UniRef90_A0AAP9ACA2", "gene": "PepB", "product": "leucyl aminopeptidase", "ec_ids": [ "3.4.11.1" ], "uniref50_id": "UniRef50_A0AAP9ACA2", "cog_id": "COG0260", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAP9ACA2", "EC:3.4.11.1", "UniRef:UniRef50_A0AAP9ACA2", "COG:COG0260", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ACA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ACA2" ], "product": "Leucyl aminopeptidase" } }, "id": "GPKLONFDEH_1061", "locus": "GCA000006625_01052" }, { "type": "cds", "sequence": "contig_1", "start": 626683, "stop": 626856, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTAATTCGCAATTTTGATAATTTGCCACTACGATTGCAAACAGCAAAAATTACAAATGAACAGTTTGCTAATATTATTAATGAAATCAATAGTGCTGATTATGATATTGATGAATATCGTCATGAAACAAGATTAACATCAAGAACCCAAAATTTTTTTAATAAATAA", "aa": "MLLIRNFDNLPLRLQTAKITNEQFANIINEINSADYDIDEYRHETRLTSRTQNFFNK", "aa_hexdigest": "72b085683ba5c6c51732b7c19ddf70cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6860.593500000001, "isoelectric_point": 5.636461448669433 }, "id": "GPKLONFDEH_1062", "locus": "GCA000006625_01053" }, { "type": "cds", "sequence": "contig_1", "start": 626884, "stop": 627393, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01580.24" ], "nt": "ATGTTAATTTCTAACATTATTATTAGTGCTTTATATACTAAATCACCTAATGAATTAGAATTATTTATTATTAATAATGGTTCAAAATCATTAAAAGAATTTGCTAAATTAAAGCATACAAAAAAAAGCGTTGATCATGATGATTTTGAAAATGTTATTAATTTATTAAGGGAAATTATGAATGATATTAACAAGCAAAATACATTATTTGCTGATAATAATGTTGATAATTTAGATGAATATAATCTAAAAAATCAAAATCAAAAATTACCTAAAAAATTAATCATTATTTCTGAATATGTTGAGATAGTAAGTAGTCAATTTAATACACGTTTTGATACCTTAATTCGCAATATAGCAAATATTGCTAAAAAACACGGAATCGTTTTAATAATTTCATCAAATATAACTAACGAAAGCACAGTTTCTTTTAAAAATGTATTTGATTATACCATTGTATTAAAATTAAATAATCCGTATGAAAGTATTTTATTAACTGATCGTAATTGA", "aa": "MLISNIIISALYTKSPNELELFIINNGSKSLKEFAKLKHTKKSVDHDDFENVINLLREIMNDINKQNTLFADNNVDNLDEYNLKNQNQKLPKKLIIISEYVEIVSSQFNTRFDTLIRNIANIAKKHGIVLIISSNITNESTVSFKNVFDYTIVLKLNNPYESILLTDRN", "aa_hexdigest": "716bf21fb02d96fe61cc041a66699506", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01580.24", "name": "FtsK_SpoIIIE", "length": 163, "aa_cov": 0.9408284023668639, "hmm_cov": 0.8220858895705522, "evalue": 2.016608575452717e-12, "score": 42.594505310058594, "start": 3, "stop": 161 } ], "seq_stats": { "molecular_weight": 19475.070000000007, "isoelectric_point": 6.838435554504395 }, "id": "GPKLONFDEH_1063", "locus": "GCA000006625_01054" }, { "type": "cds", "sequence": "contig_1", "start": 627457, "stop": 628053, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGCAAAAAGAAGTTATCCAAGAACAAAATTTACAAATTAAAAATTTTAATGATCGGCAAACTAATTCTTCATGTATCACAAAAAATGATAAACTAAAAACCACTATTTTAGAACCAATTGTTAATGATATTAACCAATTCCGAGAAAATCCATCAACAATCAAGAATAACAAGTACAATTTTTATCCACGAATTGATATTATAGATGGCAAAAGTCAAGATTATTTGCATGAACTTAAATCAAAAGGTGAAAATTTAAAATTAATTATCGATAAATTTTTAGAAAATAACCAATTAGAATATCATTTTAATGCAATTAATCCTTATTTTTCAAATGTTGAAATTGTTTATCAGTTTACTCGTTCATCATTAAACAATTTTTTAAAAAATTTTTTAAATTTAATGAAACAAGCAGTTAATGATACAGATGATTATGAAATCAATATTTATAATGACAATGACTTTTTAATCATTCAAGCCAAAGTCAAAGATTTAAATCCTCAAATTAATATTAAAGATATTCTAACTAATTTAGAATACAATGATAAATTATGTTTAGGAATTGGAAAATTAAAAGAAAGAAAAGTAGTTTGA", "aa": "MEQKEVIQEQNLQIKNFNDRQTNSSCITKNDKLKTTILEPIVNDINQFRENPSTIKNNKYNFYPRIDIIDGKSQDYLHELKSKGENLKLIIDKFLENNQLEYHFNAINPYFSNVEIVYQFTRSSLNNFLKNFLNLMKQAVNDTDDYEINIYNDNDFLIIQAKVKDLNPQINIKDILTNLEYNDKLCLGIGKLKERKVV", "aa_hexdigest": "da247066df931da7b5178d7500c20e6f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23403.26580000003, "isoelectric_point": 5.788789939880371 }, "id": "GPKLONFDEH_1064", "locus": "GCA000006625_01055" }, { "type": "cds", "sequence": "contig_1", "start": 628399, "stop": 628614, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTTTATATTATTACACTAATTTTATTTGCTTTAATTATTGGTGCAGTTGGTTATGGAATTATCACAAATTCATTAACTTATAAATTCATTATTAATACAAATCAACAAATGATTAATAAGGAAATACTTAATTTACATAAAGATAATTTCGAATATATTAGTTTAGTAAATTTACACTCAAAAAAATTACATTTATGTGAGTGGTGA", "aa": "MIVFYIITLILFALIIGAVGYGIITNSLTYKFIINTNQQMINKEILNLHKDNFEYISLVNLHSKKLHLCEW", "aa_hexdigest": "154561600ba5e9597d96d9755216c956", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8299.808499999997, "isoelectric_point": 7.906612205505372 }, "id": "GPKLONFDEH_1065", "locus": "GCA000006625_01056" }, { "type": "cds", "sequence": "contig_1", "start": 628702, "stop": 628986, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E4F2E94", "UniRef:UniRef90_UPI001E4F2E94" ], "nt": "ATGGTTAGTGACAATAAAAACACATTAAATAAGGAAACAATTAGTTTTTATAGTTATGACAATCAACTAGATGCACAAAAAACAGAATCAAACGGAAAAGAACAAGAAAAAAGCAATTTAAAGACAAAAAAATTCAAAGTTCAAAAAAATTATTTTTTTAATAAAAAAAAGCTTATTGGAATTTGTTTTTTTATATTAAGCTTTTTAATTTTAATACTTGCTTTTTTAAAAGCCCCATATACAGGAGCGATTTTAGACAATGTTTTAGAATTTTTTTTTGGATGA", "aa": "MVSDNKNTLNKETISFYSYDNQLDAQKTESNGKEQEKSNLKTKKFKVQKNYFFNKKKLIGICFFILSFLILILAFLKAPYTGAILDNVLEFFFG", "aa_hexdigest": "8236c1a2ddbe1d88fc14d414e4f0e993", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001E4F2E94", "query_cov": 0.9893617021276596, "subject_cov": 0.9117647058823529, "identity": 1.0, "score": 174.0, "evalue": 5.41e-55, "valid": true, "uniref50_id": "UniRef50_UPI001E4F2E94" }, "pscc": { "uniref50_id": "UniRef50_UPI001E4F2E94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E4F2E94" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10935.575899999989, "isoelectric_point": 9.353089332580566 }, "id": "GPKLONFDEH_1066", "locus": "GCA000006625_01057" }, { "type": "cds", "sequence": "contig_1", "start": 629003, "stop": 629302, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17770.6" ], "nt": "ATGGGCGAGAATGGTGTTATTAATTTAATTGTTATTTTTGATAAACAATTCCAAAAACTATTTAATTATGTTGAATTTGATTATTGTGGAGTTGTTAATAATGACGCTCAAATTAAAGAAATTGAAGAAACATTTAAAAAACGTATGGGTGAATGTTTAGTTTATGATGAACGTAAACGCTTAATACTAAAAGATACCAAAGCAAATTTAAAACGTTTATTAACTAAATTATTTGAAAAGAAATTTAATAAGCGTCCATTAGTTTTGCCAACAGTAGTTGACTGTTATAAAACAAAATAA", "aa": "MGENGVINLIVIFDKQFQKLFNYVEFDYCGVVNNDAQIKEIEETFKKRMGECLVYDERKRLILKDTKANLKRLLTKLFEKKFNKRPLVLPTVVDCYKTK", "aa_hexdigest": "a4dc15b57a17e34d1f3bb87ed91bdadd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17770.6", "name": "RNase_J_C", "length": 95, "aa_cov": 0.9494949494949495, "hmm_cov": 0.9894736842105263, "evalue": 8.299967607547609e-10, "score": 35.219783782958984, "start": 1, "stop": 94 } ], "seq_stats": { "molecular_weight": 11768.76969999998, "isoelectric_point": 9.278306007385254 }, "id": "GPKLONFDEH_1067", "locus": "GCA000006625_01058" }, { "type": "cds", "sequence": "contig_1", "start": 629465, "stop": 630673, "strand": "-", "frame": 3, "gene": "rnjA", "product": "Ribonuclease J C-terminal domain-containing protein", "db_xrefs": [ "COG:COG0595", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A2C9DYT9", "UniRef:UniRef90_A0A2C9DYT9" ], "nt": "ATGGCTAAAATTAATTTTTTATCGTTAGGAGGACAAGATGAAAGAGGAAAATCTTGCTTTGTTCTTGAAGTGAACGATGATATTTTTATTTTTAATGCTGGTGCGAAAATTCCAACAAGTGATGTTTTTGGCGTAAATATGATTGTTTGTGATTATTCTTATTTAGAAAAAAACGCAAAACGCGTTAAGGGAATTTTTATTGGTACACCAACATTTAATAATGTTATGGGAATTAAATTATTACTAGGACAAGTAGGTTATAAAATCCCAATTTACACAAGCCCAATAGGAGCAATTGTTATTAAAAAAATTTTTGAACAAAAAGTAAACAATAAAAAAATTGAACCTAATATTATTGAGCTAGATCCAATTTCAGATAAGAAAATTGGATCAATTTATGTTACATCTTTTAAAGTTTCAAACTCAATGCCTCATTCATATGGATTTGTATTAAAAACAAGCGATGGAGCAATTGTTTATGTTGATGAGTTTATTATTTCAAATGATAAAAATAAAACCTTTGATTCACAAATTAATCTTTTAAATAACATTACTAAAAATAATACTCTAGCTTTGATTGTAGGAATGGGTCAAGCAGGAAATCCTTATTTTACTGCTCCCAATCATAAAAATAAGGCTTTTTATGAAGCTGCATTGCAAAATACTAAAAATCGTTTAATTGTTGGTTGTTATAGCAATGATGCATATAGTATTTTTACTTTGGCAACAATTGCAAAACAACAAAATCGCCCCTTTATTGTTTATAGTAATAACTTCATTAACACTTTTATTGGTGTATTGAAATTGAAATTATTTAATAGTAAAAATTTAATTTCTTTACCGGTTTCTGAAATTAATAATTCAAAAAATGCAATTATTGTGGTAATTGAAAATCAAGATACTCTTTTTTCAAAATTAAATAAAATTCTACATAACGAAGATAAATATGTTAATTTGTCTTCTGATGATCAACTAATATTAGGCGTTGTTATTACACCAGGTTTTGAAATGTTAGCAGCTGAACTAAGTGATGAAGTAGGTCGTTTAGACATTCCTTATAAAGCATTACCTAAAACAGTTTTACCAATGACACAATCTGATGAAGATTTAAAACATCTAATTAATTTTTTACAACCAAAATTTTTAATTCCAATTAATGGATTATATAAAACTGAGGTTAAATTTAGTTCTACAGTTACAACATCTTGA", "aa": "MAKINFLSLGGQDERGKSCFVLEVNDDIFIFNAGAKIPTSDVFGVNMIVCDYSYLEKNAKRVKGIFIGTPTFNNVMGIKLLLGQVGYKIPIYTSPIGAIVIKKIFEQKVNNKKIEPNIIELDPISDKKIGSIYVTSFKVSNSMPHSYGFVLKTSDGAIVYVDEFIISNDKNKTFDSQINLLNNITKNNTLALIVGMGQAGNPYFTAPNHKNKAFYEAALQNTKNRLIVGCYSNDAYSIFTLATIAKQQNRPFIVYSNNFINTFIGVLKLKLFNSKNLISLPVSEINNSKNAIIVVIENQDTLFSKLNKILHNEDKYVNLSSDDQLILGVVITPGFEMLAAELSDEVGRLDIPYKALPKTVLPMTQSDEDLKHLINFLQPKFLIPINGLYKTEVKFSSTVTTS", "aa_hexdigest": "f16099ec92d1fea85d01cca8bc071e34", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rnjA" ], "truncated": "5-prime", "pseudogene": { "start": 629162, "stop": 630673, "observations": { "mutation": [ 629467 ], "stop": [ 629467 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYT9", "query_cov": 1.0, "subject_cov": 0.7178571428571429, "identity": 0.948, "score": 758.0, "evalue": 1.88e-273, "gene_start": 1, "gene_end": 402, "reference_start": 1, "reference_end": 402, "reference_sequence": "MAKINFLSLGGQDERGKSCFVLEVNDDIFIFNAGAKIPTSDVFGVNMIVCDYSYLEKNAKRVKGIFIGTPTFNNVMGIKLLLAQVGYKIPIYTSPIGAIVVKKIFEQKVNNKKIEPNIIELDPISDKKIGSIYVTAFKVSNSMPHSYGFVLKTSDGAIVYIDEFIISNDKNKTFDSQINALNNITKNNTLALIVGMGQAGNPCFTAPNHKNKGFYESILQNTKNRLIVGCYSNDAYSIFTLATIAKQQNRPFIVYSNNFINTFVGVLKLKLFNSKNLISLPVSEINNSKNAIIVVIENQDTLFPRLNKILHNEDKHITLTSEDQLVLGVVITPGFEMLAAQLSDEVGRLDIPYKALPKTVLPMTQSDEDLKHLINFLQPKYLIPINGLYKTEVKFTSTVTTSWIKSDQIISISNGELFSIEDKVLNPKPQVIELEDKYISSFDALDVGANILFERAQMGENGVINLIVIFDKQFQKLFNYVEFDYCGVVNDDSQVRAQVKEIEETFKKRMGECLVYDDRKRLVLKDTKASLKRLLTKLFEKKFNKRPLVLPTVVDCYKTK" }, "paralog": false, "identity": 0.9368836291913215, "score": 945.0, "evalue": 0.0, "description": "Internal stop codon at 629467. Nonsense mutation around 629467.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYT9", "gene": "RnjA", "product": "Ribonuclease J C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A2C9DYT9", "cog_id": "COG0595", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYT9", "UniRef:UniRef50_A0A2C9DYT9", "COG:COG0595", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYT9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYT9" ], "product": "Ribonuclease J C-terminal domain-containing protein" } }, "id": "GPKLONFDEH_1068", "locus": "GCA000006625_01059" }, { "type": "cds", "sequence": "contig_1", "start": 630813, "stop": 631691, "strand": "+", "frame": 3, "gene": "sunT", "product": "ABC Transporter", "db_xrefs": [ "COG:COG2274", "COG:V", "SO:0001217", "UniRef:UniRef50_Q9PPY0", "UniRef:UniRef90_Q9PPY0" ], "nt": "ATGAAAATTGTTCAACAAACTTCTGATAATGAATGTGGTGTGTGTGTTATTAACATGTTAGCAAATTATTATCATAATAAAACAATTGATAAAAACATCATTCTTCAAAAAGCAAATTTAACAAAAAATGGTTTGAGTTTGCAAGAACTTGAAAACTTAGCAAATGAATTTAATTTAGATGCACAATCATTTGAATGTAGTTTTGAGGAGTTAATTGATGAAAAAATCAATGACTATTTTATAGCTTTAATTAACAAAAACGGTTTGAATCATTTTGTTATTGTTAAAAATTATAACAAAAATTTTTTTCGTATTTATGATCCAGAAAATAAAATATATGAATTAAATAATGAAGAATTTAAAAAAATATTTTTAAATATAATTGTGCGTGTTTCTAAAAATCATCAAATTTTTGACCTTCCCATATTTAAAGAATCTTATTTAAAAAATATTAAACTCTCATCGTTAATTATCATATTATTTATAGAGTTATTAAATATTCCTTTAAATATTTTTTTAAGTAAAATTGTAAACTTACTAATAGATTTAGTTTTAATTGACACACAAATTAAAAACTTAACATACTTATTTATTGTTTTTAGTTTATTTTATATTAGTAATGCTTTCAAAGATTTACTAATTACTATATACATAAATAAAATTAGTAATAATATTTTTTCTAATATTAATAATAAAATAGTTAATAATTTAGCTAATAAATCAATTATTTTTTTTAATAAAACAAATTTAGGTGAATTAAGCTCGATTCAGATTCATTTGAATAATATAATTCATTTTCTATTAGTTAGTCGTATAAAATTATTAGTTAATACTTTATTTGCAATTTGTATTATTTGTTTATTAGGTTTACAAAATTGA", "aa": "MKIVQQTSDNECGVCVINMLANYYHNKTIDKNIILQKANLTKNGLSLQELENLANEFNLDAQSFECSFEELIDEKINDYFIALINKNGLNHFVIVKNYNKNFFRIYDPENKIYELNNEEFKKIFLNIIVRVSKNHQIFDLPIFKESYLKNIKLSSLIIILFIELLNIPLNIFLSKIVNLLIDLVLIDTQIKNLTYLFIVFSLFYISNAFKDLLITIYINKISNNIFSNINNKIVNNLANKSIIFFNKTNLGELSSIQIHLNNIIHFLLVSRIKLLVNTLFAICIICLLGLQN", "aa_hexdigest": "904fe3950a25a1156ef07109a1dbb466", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "sunT" ], "truncated": "3-prime", "pseudogene": { "start": 630813, "stop": 631994, "observations": { "mutation": [ 631689, 631752 ], "stop": [ 631689, 631752 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPY0", "query_cov": 1.0, "subject_cov": 0.44376899696048633, "identity": 1.0, "score": 530.0, "evalue": 8.41e-184, "gene_start": 1, "gene_end": 292, "reference_start": 1, "reference_end": 292, "reference_sequence": "MKIVQQTSDNECGVCVINMLANYYHNKTIDKNIILQKANLTKNGLSLQELENLANEFNLDAQSFECSFEELIDEKINDYFIALINKNGLNHFVIVKNYNKNFFRIYDPENKIYELNNEEFKKIFLNIIVRVSKNHQIFDLPIFKESYLKNIKLSSLIIILFIELLNIPLNIFLSKIVNLLIDLVLIDTQIKNLTYLFIVFSLFYISNAFKDLLITIYINKISNNIFSNINNKIVNNLANKSIIFFNKTNLGELSSIQIHLNNIIHFLLVSRIKLLVNTLFAICIICLLGLQNWSLLLISLGFSLLNFIIGLINWKYFKIHSLKIINIENSLFIKYQNFIKLINLEYNIDKYNNYINSYKKQFDELIIFKNKITIFKSNVNFYLSIINNIGLLILLTYLIINTNYDYKVISVITYIVFLHQNLNNLFFGIFNFFNELLNFKNGKQIVEKILYIDNYEEHDGIQINKITSCKITENQKEFEFSKSCLIIGKSGVGKTTLLKNFLNFNNKNLFFNNLNVNNINLNQLKELIIYHPTNPLINDFDINWFIDKNEEIINALKIVIFITKIDWSLNNNFEHNINLSTGQQQILAFLNLLKYKNKLLLLDEPLAHVDNDNKKIILNSILPIILKNNFVIYVSHDHYLKKYFDQIINLNDEKMDKY" }, "paralog": false, "identity": 0.9949109414758269, "score": 748.0, "evalue": 3.67e-272, "description": "Internal stop codon at 631689, 631752. Nonsense mutation around 631689, 631752.", "psc": { "uniref90_id": "UniRef90_Q9PPY0", "gene": "SunT", "product": "ABC Transporter", "uniref50_id": "UniRef50_Q9PPY0", "cog_id": "COG2274", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPY0", "UniRef:UniRef50_Q9PPY0", "COG:COG2274", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPY0" ], "product": "ABC Transporter" } }, "id": "GPKLONFDEH_1069", "locus": "GCA000006625_01060" }, { "type": "cds", "sequence": "contig_1", "start": 631986, "stop": 632513, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307F29B9", "UniRef:UniRef90_UPI00307F29B9" ], "nt": "TTGATTTTATTAACTTATCTAATCATTAATACCAATTATGATTATAAAGTTATTAGCGTAATCACTTATATAGTTTTTTTACATCAAAATCTAAATAATTTGTTTTTTGGTATCTTTAATTTTTTTAATGAATTATTAAATTTTAAAAATGGAAAACAAATTGTTGAAAAAATTTTGTATATTGATAATTATGAAGAACATGATGGTATACAAATTAATAAAATTACATCATGTAAAATTACCGAAAACCAAAAGGAATTTGAATTTAGCAAATCTTGTTTAATCATAGGAAAAAGTGGTGTTGGAAAAACAACATTATTAAAAAATTTTTTAAACTTTAATAATAAAAATTTATTTTTTAATAACTTAAATGTAAATAATATAAATTTAAATCAACTTAAAGAATTAATTATCTATCATCCAACAAATCCTTTAATAAATGATTTTGATATAAATTGGTTTATAGATAAAAATGAAGAAATTATTAATGCTTTAAAAATAGTTATTTTTATAACAAAGATCGATTGA", "aa": "MILLTYLIINTNYDYKVISVITYIVFLHQNLNNLFFGIFNFFNELLNFKNGKQIVEKILYIDNYEEHDGIQINKITSCKITENQKEFEFSKSCLIIGKSGVGKTTLLKNFLNFNNKNLFFNNLNVNNINLNQLKELIIYHPTNPLINDFDINWFIDKNEEIINALKIVIFITKID", "aa_hexdigest": "e33951c9a00d8a0cac1833f71bae1ed9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 631686, "stop": 632789, "observations": { "mutation": [ 631689, 631752, 632511 ], "stop": [ 631689, 631752, 632511 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00307F29B9", "query_cov": 1.0, "subject_cov": 0.43859649122807015, "identity": 0.9940000000000001, "score": 306.0, "evalue": 1.47e-101, "gene_start": 1, "gene_end": 175, "reference_start": 133, "reference_end": 307, "reference_sequence": "MNNIIHFLLVSRIKLLVNTLFAICIICLLGLQNWSLLLISLGFSLLNFIIGLINWKYFKIHSLKIINIENSLFIKYQNFIKLINLEYNIDKYNNYINSYKKQFDELIIFKNKITIFKSNVNFYLSIINNIGLLILLTYLIINTNYDYKVISVITYIVFLHQNLNNLFFGIFNFFNELLNFKNGKQIVEKILYIDNYEEHDGIQINKITSCKITENQKEFEFSKSCLIIGKSGVGKTTLLKNFLNFNNKNLFFNNLNVNNINLNQLKELIIYHPTNPLINDFDINWFIDKNEEIINALKIVIFITKIDWSLNNNFEHNINLSTGQQQILAFLNLLKYKNKLLLLDEPLAHVDNDNKKIILNSILPIILKNNFVIYVSHDHYLKKYFDQIINLNDEKMDKY" }, "paralog": false, "identity": 0.9918256130790191, "score": 686.0, "evalue": 6.37e-254, "description": "Internal stop codon at 631689, 631752, 632511. Nonsense mutation around 631689, 631752, 632511.", "psc": { "uniref90_id": "UniRef90_UPI00307F29B9", "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI00307F29B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00307F29B9", "UniRef:UniRef50_UPI00307F29B9" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00307F29B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00307F29B9" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "GPKLONFDEH_1070", "locus": "GCA000006625_01061" }, { "type": "cds", "sequence": "contig_1", "start": 632764, "stop": 633129, "strand": "+", "frame": 1, "gene": null, "product": "Transmembrane protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C9D", "UniRef:UniRef100_A0AAC9T2J2", "UniRef:UniRef50_A0AAC9T2J2", "UniRef:UniRef90_A0AAC9T2J2" ], "nt": "ATGATGAAAAAATGGATAAGTATTAGTCTTTTGGGTTTTTTATTATCTTTATTAATAGCAATTGGTGTAATGTTTGGATTTAAAAATAAAAAATATTTTAAAACGACATTAAACATTCAAACAAAATTATCTATAATCACTAAAAACAATTATGAAAAAATTAAAACCAAAAAACAACTAGAAATTAAAATTAAAAATTTAACTTATTATGTTAAATTTAAATTTTTAAAACAAACTCATGATTATTGCCTAATAAAAATCTTTCTTAGTTTAAAAACAGATCAAAAAATGATTGATATTAGTATATACACTAATACTCAACCATTAATTGAATCTATAATTAATAATTTCAAATTATACAATTAA", "aa": "MMKKWISISLLGFLLSLLIAIGVMFGFKNKKYFKTTLNIQTKLSIITKNNYEKIKTKKQLEIKIKNLTYYVKFKFLKQTHDYCLIKIFLSLKTDQKMIDISIYTNTQPLIESIINNFKLYN", "aa_hexdigest": "ffb82dfbbbee588a01fe434ec4d4c16e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1C9D", "uniref100_id": "UniRef100_A0AAC9T2J2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1C9D", "UniRef:UniRef100_A0AAC9T2J2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T2J2", "uniref90_id": "UniRef90_A0AAC9T2J2" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T2J2", "product": "Transmembrane protein", "uniref50_id": "UniRef50_A0AAC9T2J2" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2J2" ], "product": "Transmembrane protein" }, "genes": [], "id": "GPKLONFDEH_1071", "locus": "GCA000006625_01062" }, { "type": "cds", "sequence": "contig_1", "start": 633118, "stop": 633351, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTTTAATAAGCACTAGTGGGGACTTAGTATTTAGTGCGATTAAACGTAATTATCATACCAAAGATTATAGTAATTTAATTCCTGGCCATGGTGGATTATTAGATCGTATAGATTCACATTCATTTGTAATATCAATCATGTTCATTTTTACACTTCTTATTACAGGCAGTTTACAAGATGAAAATTTATTTCCTAACATTCAAAAATTTTTTATTCAATTAATTGTATAA", "aa": "MSLISTSGDLVFSAIKRNYHTKDYSNLIPGHGGLLDRIDSHSFVISIMFIFTLLITGSLQDENLFPNIQKFFIQLIV", "aa_hexdigest": "669fee5b4b6a45081dc7a0cb6f83a3c4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8703.004199999994, "isoelectric_point": 6.222528266906737 }, "id": "GPKLONFDEH_1072", "locus": "GCA000006625_01063" }, { "type": "cds", "sequence": "contig_1", "start": 633382, "stop": 633672, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTGTTTTTTTAAGTGATACATTTGCATATGTTGGTGGTGTTTTATTTGGTAAAACAAAATTAGCACCAAAAATAAGTCCTAACAAAACAGTTGAAGGATTAATTTTTGGTTTAGTAATTGCAACAATTGTTATTATGTTAATAATTTTTGGTTTGTCATATGTGCCAATTAAACCTAAAAACGGCATAGTGTTAAAATCACAAAATGTTTTATATAATATTTTTGGTTTAGATTTTTCTAGTTCAAATGAAAAAAATCTTACATTGCATAAACAAGCATTATGA", "aa": "MMIVFLSDTFAYVGGVLFGKTKLAPKISPNKTVEGLIFGLVIATIVIMLIIFGLSYVPIKPKNGIVLKSQNVLYNIFGLDFSSSNEKNLTLHKQAL", "aa_hexdigest": "dafc4041d9a622f31fc8a551c4e55304", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10503.511099999989, "isoelectric_point": 9.699349021911619 }, "id": "GPKLONFDEH_1073", "locus": "GCA000006625_01064" }, { "type": "cds", "sequence": "contig_1", "start": 633956, "stop": 634093, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATATTAAAAACAATTGTTAAGATGATTAATGTTATCTTATTATTATCAAAAATGACTTTATTCATTTCATACGAACCAATACATATTAATGGTAATAATCATATTAATAAAACAATTGCAATCCCTTGTTGA", "aa": "MQILKTIVKMINVILLLSKMTLFISYEPIHINGNNHINKTIAIPC", "aa_hexdigest": "f1f0646574bb5c3101209075460887e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5135.2691, "isoelectric_point": 9.516774559020995 }, "id": "GPKLONFDEH_1074", "locus": "GCA000006625_01065" }, { "type": "cds", "sequence": "contig_1", "start": 634265, "stop": 634756, "strand": "-", "frame": 3, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023", "SO:0001217", "UniRef:UniRef50_Q9PPX7", "UniRef:UniRef90_Q9PPX7" ], "nt": "ATGCACAATGAATTTATTAAATTGCGAACAGGAAGAGCTACGCCTGCTATTTTAGATGGTATTTTAGTTAATTATTATGGTTCAATGATGCCAATTAATCAATTAGCAAATATTTCAGTTCCAGAACCACGTGTTTTAGCAATTAAACCTTATGATAGAAGTAGTATTAAAGATATTGCTACTGCAATTAACGCATCAAATTTAGGTGTTAATCCTCAAGTAGATGTTGATATTATTCGTTTAACGTTCGCTGCTCCAACAGAAGAAGTAAGAAAAAATTTAGTTAAAAAAGCAAAACAGGTTGGCGAAGAAGCTAAAATTCGTGTACGTCATATCCGTCAAGAAGCACAAGATTTATTCAAAAAAGATTCAACAACTATTGAAGACGATAAAAAGTTTTTTCAAACAGAATTAGATAATTTAACAAAAGAATTAAATAAGGAAATCGAAACAGTTGTAAGTCATAAAGAAAAAGACATAATGACTGTTTAA", "aa": "MHNEFIKLRTGRATPAILDGILVNYYGSMMPINQLANISVPEPRVLAIKPYDRSSIKDIATAINASNLGVNPQVDVDIIRLTFAAPTEEVRKNLVKKAKQVGEEAKIRVRHIRQEAQDLFKKDSTTIEDDKKFFQTELDNLTKELNKEIETVVSHKEKDIMTV", "aa_hexdigest": "5e7fb3641b32aa1cbb6316fba742bf16", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PPX7", "query_cov": 1.0, "subject_cov": 0.8907103825136612, "identity": 1.0, "score": 307.0, "evalue": 3.37e-105, "valid": true, "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_Q9PPX7", "cog_id": "COG0233", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPX7" ], "product": "Ribosome-recycling factor" }, "genes": [ "frr" ], "id": "GPKLONFDEH_1075", "locus": "GCA000006625_01066" }, { "type": "cds", "sequence": "contig_1", "start": 634834, "stop": 635352, "strand": "-", "frame": 1, "gene": "pyrH", "product": "UMP kinase", "db_xrefs": [ "COG:COG0528", "COG:F", "EC:2.7.4.22", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210", "KEGG:K09903", "SO:0001217", "UniRef:UniRef50_Q9PPX6", "UniRef:UniRef90_Q9PPX6" ], "nt": "ATGGATCGTAATTTAGCTGATAATATGGGTATGATGGCAACTATTATAAATGGGTTAGCTTTAGAAAATGCTTTAAATCATTTGAATGTTAACACTATTGTTTTATCTGCAATTAAATGTGATAAATTAGTTCATGAATCATCTGCTAATAATATAAAAAAAGCTATTGAAAAAGAACAAGTAATGATTTTTGTGGCTGGAACAGGATTTCCATATTTTACAACTGATTCGTGTGCAGCAATTAGAGCTGCTGAAACAGAATCTAGTATAATTTTAATGGGCAAAAATGGTGTTGATGGTGTCTATGATAGTGATCCAAAAATTAATCCTAATGCACAATTTTATGAACATATTACATTTAATATGGCCCTTACTCAAAATTTAAAAGTTATGGATGCAACAGCTTTAGCTTTGTGTCAAGAAAATAATATAAATTTATTAGTTTTTAATATTGATAAACCAAATGCAATTGTTGATGTTTTAGAGAAAAAAAATAAATACACAATTGTTTCAAAATAA", "aa": "MDRNLADNMGMMATIINGLALENALNHLNVNTIVLSAIKCDKLVHESSANNIKKAIEKEQVMIFVAGTGFPYFTTDSCAAIRAAETESSIILMGKNGVDGVYDSDPKINPNAQFYEHITFNMALTQNLKVMDATALALCQENNINLLVFNIDKPNAIVDVLEKKNKYTIVSK", "aa_hexdigest": "ffcfdefa481a42ef37457fc7531f6d56", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pyrH" ], "truncated": "3-prime", "pseudogene": { "start": 634834, "stop": 635541, "observations": { "mutation": [ 635382 ], "stop": [ 635382 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPX6", "query_cov": 1.0, "subject_cov": 0.671875, "identity": 1.0, "score": 338.0, "evalue": 3.97e-116, "gene_start": 1, "gene_end": 172, "reference_start": 85, "reference_end": 256, "reference_sequence": "MGSSHHHHHHSSGLVPRGSHMMRKQRIVIKISGACLKQNDSSIIDFIKINDLAEQIEKISKKYIVSIVLGGGNIWRGSIAKELDMDRNLADNMGMMATIINGLALENALNHLNVNTIVLSAIKCDKLVHESSANNIKKAIEKEQVMIFVAGTGFPYFTTDSCAAIRAAETESSIILMGKNGVDGVYDSDPKINPNAQFYEHITFNMALTQNLKVMDATALALCQENNINLLVFNIDKPNAIVDVLEKKNKYTIVSK" }, "paralog": false, "identity": 0.9957446808510638, "score": 454.0, "evalue": 2.74e-164, "description": "Internal stop codon at 635382. Nonsense mutation around 635382.", "psc": { "uniref90_id": "UniRef90_Q9PPX6", "gene": "pyrH", "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_Q9PPX6", "cog_id": "COG0528", "cog_category": "F", "kegg_orthology_id": "K09903", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPX6", "EC:2.7.4.22", "UniRef:UniRef50_Q9PPX6", "COG:COG0528", "COG:F", "KEGG:K09903", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPX6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPX6" ], "product": "Uridylate kinase" } }, "id": "GPKLONFDEH_1076", "locus": "GCA000006625_01067" }, { "type": "cds", "sequence": "contig_1", "start": 635380, "stop": 635541, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGAAAACAACGAATTGTAATTAAAATTTCTGGAGCTTGCTTAAAACAAAATGATAGTTCAATAATTGATTTTATAAAGATTAATGATTTAGCAGAACAAATTGAAAAAATTTCTAAGAAATATATTGTATCAATTGTTTTAGGTGGTGGCAATATTTGA", "aa": "MRKQRIVIKISGACLKQNDSSIIDFIKINDLAEQIEKISKKYIVSIVLGGGNI", "aa_hexdigest": "e2ba862d4e64e41de171261b71c8e887", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5902.968799999999, "isoelectric_point": 9.579889106750485 }, "id": "GPKLONFDEH_1077", "locus": "GCA000006625_01068" }, { "type": "cds", "sequence": "contig_1", "start": 635907, "stop": 636320, "strand": "-", "frame": 2, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "SO:0001217", "UniRef:UniRef50_Q9PPX5", "UniRef:UniRef90_Q9PPX5" ], "nt": "TTGGCCAAAAAAATAAATAATAAAGCAATTCTTATTGAGGTTAATTGTCAAACTGACTTTGTTGCTAAGAATGAAAATTTTTTAGCTTATGCTAATCAAATTTTAGAAGAAGCATTAGCTAAGGTTGAAAATAAAGAAGATTTTGATAAATTAATTATAAATGGAAAACCAATTGCTGAATCAGGATTAGATTTAACAGCATATATTGGTGAAAAAATCGTTTTTAGACGTGGAGAAATTTTAAAAGCTAATGATGACCAAACTTTAGGTGTTTATACTCACAATAACAACAGAGTTGCTGCTATTATTTTAGTTGATGGAAAAGTTGAAGATGAAGTTGTTCGAAATGTAGCAATGCATGCTGCAGCAATGCGTCCACGTTATTTAAATGAAAAAGTTGTTGATAAGTTATGA", "aa": "MAKKINNKAILIEVNCQTDFVAKNENFLAYANQILEEALAKVENKEDFDKLIINGKPIAESGLDLTAYIGEKIVFRRGEILKANDDQTLGVYTHNNNRVAAIILVDGKVEDEVVRNVAMHAAAMRPRYLNEKVVDKL", "aa_hexdigest": "0d771586994ba3e658224ddfeb242752", "start_type": "TTG", "rbs_motif": null, "genes": [ "tsf" ], "truncated": "both", "pseudogene": { "start": 635625, "stop": 636500, "observations": { "mutation": [ 635909, 636389 ], "stop": [ 635909, 636389 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPX5", "query_cov": 1.0, "subject_cov": 0.47079037800687284, "identity": 0.993, "score": 265.0, "evalue": 1.73e-87, "gene_start": 1, "gene_end": 137, "reference_start": 61, "reference_end": 197, "reference_sequence": "MTKAELVKELRIRTQASMSECIKALDASENDIEKAIVWLRENGAIKAANKLKNAATDGVVLAKKINNKAILIEVNCQTDFVAKNENFLAYANQILEEALAKVENKEDFDKLIINGKPIAESGLDLTAYIGEKIVFRRGEILKANDDQTLGVYTHNNNRVAAIILVDGKVEDEVVRNVAMHAAAMRPRYLNEKVVDKLWLAKEREIIVNQLEHEGKPAAFAAKIIDGRLNKILKENCLVDQSYFKQPELTIEKYLKNNNAVAVGYFSYEVGEGIEKAPQMSFADEVAAQMKK" }, "paralog": false, "identity": 0.993127147766323, "score": 554.0, "evalue": 4.6e-204, "description": "Internal stop codon at 635909, 636389. Nonsense mutation around 635909, 636389.", "psc": { "uniref90_id": "UniRef90_Q9PPX5", "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_Q9PPX5", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPX5", "UniRef:UniRef50_Q9PPX5", "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPX5" ], "product": "Elongation factor Ts" } }, "id": "GPKLONFDEH_1078", "locus": "GCA000006625_01069" }, { "type": "cds", "sequence": "contig_1", "start": 636387, "stop": 636500, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGCTGAATTAGTTAAAGAATTAAGAATAAGAACACAAGCTAGTATGAGTGAATGTATTAAAGCTTTAGATGCGTCAGAAAATGATATTGAAAAAGCAATAGTATGA", "aa": "MTKAELVKELRIRTQASMSECIKALDASENDIEKAIV", "aa_hexdigest": "acf2e89877ede61234ba62f87fe98da7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4135.7818, "isoelectric_point": 5.1446336746215815 }, "id": "GPKLONFDEH_1079", "locus": "GCA000006625_01070" }, { "type": "tRNA", "sequence": "contig_1", "start": 636648, "stop": 636731, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 63.9, "nt": "GCGCTTGTGGCGGAATGGCAGACGCGCTAGACTTAGGATCTAGTACTTTGCGGTGTAAGAGTTCAAGTCTCTTCAAGCGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 636702, 636704 ], "id": "GPKLONFDEH_18", "locus": "GCA000006625_01071" }, { "type": "tRNA", "sequence": "contig_1", "start": 636736, "stop": 636811, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 72.1, "nt": "GCTCTGTTAGCTCAGTAGGTAGAGCAACTGACTCTTAATCAGTGGGTCGCGGGTTCGAAACCCTCACAGAGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 636776, 636778 ], "id": "GPKLONFDEH_17", "locus": "GCA000006625_01072" }, { "type": "tRNA", "sequence": "contig_1", "start": 636883, "stop": 636958, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 82.3, "nt": "GCCGACTTAGCTCAATAGGTAGAGCAACTGACTTGTAATCAGTAGGTTGTGGGTTCAATTCCTATAGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 636923, 636925 ], "id": "GPKLONFDEH_16", "locus": "GCA000006625_01073" }, { "type": "tRNA", "sequence": "contig_1", "start": 636960, "stop": 637035, "strand": "-", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 85.0, "nt": "GGAGTGCTAGCTCAGTTGGGAGAGCATTTGCCTTACAAGCAAAGGGTCAGGGGTTCGAGTCCCTTGCACTCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 637000, 637002 ], "id": "GPKLONFDEH_15", "locus": "GCA000006625_01074" }, { "type": "tRNA", "sequence": "contig_1", "start": 637082, "stop": 637158, "strand": "-", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 59.0, "nt": "GGCCTGTTGGTGAAGTGACTGAACACACACGCCTTTCACGCGTGCATTCACGGGTTTGAATCCCGTACAGGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 637122, 637124 ], "id": "GPKLONFDEH_14", "locus": "GCA000006625_01075" }, { "type": "tRNA", "sequence": "contig_1", "start": 637160, "stop": 637234, "strand": "-", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 83.5, "nt": "GGCTACATAGCTCAGCGGTAGAGCAACCGGCTGTTAACCGGTTGGTCGTAGGTTCGATCCCTACTGTAGCCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 637200, 637202 ], "id": "GPKLONFDEH_13", "locus": "GCA000006625_01076" }, { "type": "cds", "sequence": "contig_1", "start": 637428, "stop": 638024, "strand": "+", "frame": 3, "gene": "fepD", "product": "Iron ABC transporter permease", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_A0AAC9T065", "UniRef:UniRef90_A0AAC9T065" ], "nt": "ATGAATAAAATAAAAAATGCTAAATCTTCGTTTCTATATTTTAAAAAAATGCATAATTTAAAATCTTTTAACAAAAAAAGAAATAGATTAAAAGGCATACAAATCATATTTATTGTTTTTCTAATAATCTTTTGTTCATTATTTTTCTTAATTGATTTATTTTTTACAGGTAATCATTTGAACGTCATTAAAAGAATGTTTGAAAATAGTTCTCTTTCAAATTCAACTTATATTTTTGTTCCTGTTGCTAATATAATTACAGGATTTAGTTTAGGAATTGGTTCAATTTCTATCCAAATTACTTCTAAAAATATTTTAGCTGGACCGTCAACACTTGGTTTTACACCAATGGCAATATTAGCATCAACCATTTCATTTATTATTACTTCAAGTGGTATTTTTTCAACAATTTTAATTTACTGTTTAGGTTTAATTTTTTCATTTATTATAATAGGTGTTAATTTTATATTAGTGAGAAATAATTTTTTAGAAAATAGTTTTAAACCTATTTTAGTTGCTTTTGGGATTGGAGCATTAGTGACGGGAATTAATATTGTTTTAATTGCAACACATGACAATTTAAAAATTGTGGGATGA", "aa": "MNKIKNAKSSFLYFKKMHNLKSFNKKRNRLKGIQIIFIVFLIIFCSLFFLIDLFFTGNHLNVIKRMFENSSLSNSTYIFVPVANIITGFSLGIGSISIQITSKNILAGPSTLGFTPMAILASTISFIITSSGIFSTILIYCLGLIFSFIIIGVNFILVRNNFLENSFKPILVAFGIGALVTGINIVLIATHDNLKIVG", "aa_hexdigest": "819f5f0b058cc166a49056f04f67869d", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "fepD" ], "truncated": "3-prime", "pseudogene": { "start": 637428, "stop": 638327, "observations": { "mutation": [ 638022 ], "stop": [ 638022 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T065", "query_cov": 1.0, "subject_cov": 0.5641025641025641, "identity": 1.0, "score": 360.0, "evalue": 4.42e-123, "gene_start": 1, "gene_end": 198, "reference_start": 1, "reference_end": 198, "reference_sequence": "MNKIKNAKSSFLYFKKMHNLKSFNKKRNRLKGIQIIFIVFLIIFCSLFFLIDLFFTGNHLNVIKRMFENSSLSNSTYIFVPVANIITGFSLGIGSISIQITSKNILAGPSTLGFTPMAILASTISFIITSSGIFSTILIYCLGLIFSFIIIGVNFILVRNNFLENSFKPILVAFGIGALVTGINIVLIATHDNLKIVGWWRFITINNTLINNYRMIVSSVLMIISTIILLFLSPYLNIIRKDYILAKSLGIKVNLIYWLVAICVVTITISSSILLGIIALLGVIVGIIIQTIFKKTHVLLLMILAGLFGSGILSFSSYINEYIPSAREMIICVFAIPVFAYILSKRKGFIK" }, "paralog": false, "identity": 0.9966555183946488, "score": 567.0, "evalue": 4.31e-207, "description": "Internal stop codon at 638022. Nonsense mutation around 638022.", "psc": { "uniref90_id": "UniRef90_A0AAC9T065", "gene": "FepD", "product": "Iron ABC transporter permease", "uniref50_id": "UniRef50_A0AAC9T065", "cog_id": "COG0609", "cog_category": "P", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T065", "UniRef:UniRef50_A0AAC9T065", "COG:COG0609", "COG:P" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T065", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T065" ], "product": "Iron ABC transporter permease" } }, "id": "GPKLONFDEH_1080", "locus": "GCA000006625_01077" }, { "type": "cds", "sequence": "contig_1", "start": 638070, "stop": 638483, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTCAAGTGTTCTAATGATTATTTCTACTATTATTTTATTATTTTTATCTCCTTATTTAAATATTATTAGAAAAGATTATATACTTGCTAAATCATTGGGGATTAAAGTCAATCTAATTTATTGGTTAGTGGCTATTTGTGTGGTAACTATTACTATATCATCATCTATATTATTGGGAATAATTGCTTTATTAGGAGTAATTGTTGGAATTATTATTCAAACAATTTTTAAAAAAACACACGTTTTATTACTAATGATACTAGCAGGACTTTTTGGTAGTGGAATCTTATCATTTTCAAGTTACATTAATGAATATATTCCTAGTGCCCGTGAAATGATTATTTGTGTTTTTGCTATACCAGTATTTGCTTACATTCTAAGTAAGAGAAAAGGATTTATTAAATAA", "aa": "MIVSSVLMIISTIILLFLSPYLNIIRKDYILAKSLGIKVNLIYWLVAICVVTITISSSILLGIIALLGVIVGIIIQTIFKKTHVLLLMILAGLFGSGILSFSSYINEYIPSAREMIICVFAIPVFAYILSKRKGFIK", "aa_hexdigest": "08dd53b31024b839991b78d2f9fb92f4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15137.601999999988, "isoelectric_point": 9.780579185485838 }, "id": "GPKLONFDEH_1081", "locus": "GCA000006625_01078" }, { "type": "cds", "sequence": "contig_1", "start": 638671, "stop": 639432, "strand": "+", "frame": 1, "gene": "fepD", "product": "Ferric anguibactin transport system permerase protein", "db_xrefs": [ "COG:COG0609", "COG:P", "SO:0001217", "UniRef:UniRef50_Q9PPX3", "UniRef:UniRef90_Q9PPX3" ], "nt": "ATGCAAATTTTTTTATCAGGTTTTATTTTAGGATTTTGTGCATATTTAATTCAAAAATTAACAAAAAACCGCTTCAGTGATACATCAATTATGGGAATTAGTAGTGTTAATATTTTAGGAGTAATTATTATTGCATTTCAGGTTGATTTTAATAATTTTAGTGCTGATAAATTATCAACATTAGAGCATACTGAACCAATAATATTTTTTATAGCACCAATTATTTTATGTATGCTCTATTATTTTACATGCAAAGAACAAAGTAGTTTTAATTATAAAAAAATGCTGATTTCAGGAGTTATTGTTAATTTTTTAAGCGTTGCATTAGGAGCAAGTATTGCTAAAAATTTACCTAAATTAGCTAATGCTTACGTTTCAAGATACACATATGGTAGTATTGAACCACAACAAGAGTCTTTTTTTATCGCTTTAGTTTTAATTATCATAGGGTTATTAATAGTTTTATTTAATTTTAAAAAAATTCAAATAGTTTCATCTAATCAAGACTTAGCTAATCAATTAGGAATCAACGTCAAATTAACTTCTGGACTTCTTTTAGTTGCAATTTGTTTAATGGTAGGAGCATCATATAGTTTGAATGGAAATTTGATATTTATAGGATTAATGGCTGGAAACATGGGTTCTGTTATTAGTAACAACCGATTTAAATCCGCTGCTATTAGTTCAGGATGTTGTGGGTCATTAATATATATGTTGTCTTTTTTATTATTTATTAAAATTTTAAATTTTGATAGTCAATGA", "aa": "MQIFLSGFILGFCAYLIQKLTKNRFSDTSIMGISSVNILGVIIIAFQVDFNNFSADKLSTLEHTEPIIFFIAPIILCMLYYFTCKEQSSFNYKKMLISGVIVNFLSVALGASIAKNLPKLANAYVSRYTYGSIEPQQESFFIALVLIIIGLLIVLFNFKKIQIVSSNQDLANQLGINVKLTSGLLLVAICLMVGASYSLNGNLIFIGLMAGNMGSVISNNRFKSAAISSGCCGSLIYMLSFLLFIKILNFDSQ", "aa_hexdigest": "ebbdaa313737822ba2d5d3311c1fad52", "start_type": "ATG", "rbs_motif": null, "genes": [ "fepD" ], "truncated": "both", "pseudogene": { "start": 638485, "stop": 639510, "observations": { "mutation": [ 638503, 638617, 639430 ], "stop": [ 638503, 638617, 639430 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPX3", "query_cov": 1.0, "subject_cov": 0.7419354838709677, "identity": 0.9209999999999999, "score": 446.0, "evalue": 4.87e-156, "gene_start": 1, "gene_end": 253, "reference_start": 63, "reference_end": 315, "reference_sequence": "MINLSTWNKTYSFKNPLKYQNKLAYLKTIITVAISIIVFIITLAWTYDFNFKQNNIALVYIVMQIFLSGFVLGFCAYFIQKLTKNRFGDTSIMGISSVNILGVIIIAFQVDFNNFSIDKLTTLQRTEPLIFFIAPIILCSIYYFTCKEESNFNYKKMLISGVIVNFLSVALGSSIAKNLPKLANAYISRYTYGSIEPQQESFFIALVLIIIGFLIIMFNFKKIQIVSSNQDLANQLGINVKLISGLLLVAICLMVGASYSLNGNLIFIGLMAGNMGSVISNNRFKSAAISSGSCGALIYMLSFLLFIKILNFDSQWLNLAIPLLISPYFIYIIVKKNKSDL" }, "paralog": false, "identity": 0.9032258064516129, "score": 603.0, "evalue": 8.63e-221, "description": "Internal stop codon at 638503, 638617, 639430. Nonsense mutation around 638503, 638617, 639430.", "psc": { "uniref90_id": "UniRef90_Q9PPX3", "gene": "FepD", "product": "Ferric anguibactin transport system permerase protein", "uniref50_id": "UniRef50_Q9PPX3", "cog_id": "COG0609", "cog_category": "P", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPX3", "UniRef:UniRef50_Q9PPX3", "COG:COG0609", "COG:P" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPX3" ], "product": "Ferric anguibactin transport system permerase protein" } }, "id": "GPKLONFDEH_1082", "locus": "GCA000006625_01079" }, { "type": "cds", "sequence": "contig_1", "start": 639583, "stop": 639810, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTCTTATTACAGGAATTAGTATACCTGTAATAACAACAAGTTGTTCTCTAATTGATTTGTTCAAAAGTAATAATGAGAAACAAAATGAATTTACTCATGGTAAACTAAAATCTAATCTTGATTACAATGTAAGTACTGCTTATTTAAATAAAAGATCTATTGCTTTAAAATTCACAATACCTTTAGATAAAAATCAAGCTATTAATATTTTTGGAACAGGATGA", "aa": "MILITGISIPVITTSCSLIDLFKSNNEKQNEFTHGKLKSNLDYNVSTAYLNKRSIALKFTIPLDKNQAINIFGTG", "aa_hexdigest": "e8f7a4ba1b528aee928785f56c5757e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8318.532799999995, "isoelectric_point": 9.251745033264157 }, "id": "GPKLONFDEH_1083", "locus": "GCA000006625_01080" }, { "type": "cds", "sequence": "contig_1", "start": 639862, "stop": 640317, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGGCAACTAACATTCATGTTATTAATTTGATTTCAGCAGCAAAACAAAATGCTTCTTTAACGCTTGGTCAATACGATTTAGAATCCCAATCCCAAGATACAAGAGCTTTTTTTAATGAAAATTTATCATTTAAATCCGCTAAAAAAACTTATAATCCACAATACTATGTAAATTATAATCCAGTAGGCACTTTTTCAATTTTGCCTGTTGGATTAAAAAACCAACTAAAAAATTATGGTATTAAAAACCCAGCCGCTTTAGCTTCTTTAAATAATTTAACAACTGCTGTTGATTTAGCTATTATCAAAATCACACCAGATATCTTACAAAAAAATAATATTGATGCTTTGAGAAATTTTGATAAAGAAGTAGGTATTGGTTTTGGTAATACTATTAGCAATCATCAAAGGTTTTATGCCGCAGGATATCCACATGATGAAAAATATAGTCGTTGA", "aa": "MATNIHVINLISAAKQNASLTLGQYDLESQSQDTRAFFNENLSFKSAKKTYNPQYYVNYNPVGTFSILPVGLKNQLKNYGIKNPAALASLNNLTTAVDLAIIKITPDILQKNNIDALRNFDKEVGIGFGNTISNHQRFYAAGYPHDEKYSR", "aa_hexdigest": "31f55ef9ae497d8953fdcf0a26d9da1d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 167, "aa_cov": 0.9403973509933775, "hmm_cov": 0.9101796407185628, "evalue": 4.677870894764875e-10, "score": 35.197975158691406, "start": 1, "stop": 142 } ], "seq_stats": { "molecular_weight": 16846.774799999977, "isoelectric_point": 9.246716499328613 }, "id": "GPKLONFDEH_1084", "locus": "GCA000006625_01081" }, { "type": "cds", "sequence": "contig_1", "start": 640417, "stop": 640566, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGACAGTTAGTAACCGTAACACAATAGGTAAAAATAATCCTACTAAATATGAAGCTTATGACTATGCAACAAAAAATTTTGATTTACATTCAGGAGCATCAGGAAGTCCAATAATTGATGACAATAATAACTATGTTGGAATTTATTGA", "aa": "MTVSNRNTIGKNNPTKYEAYDYATKNFDLHSGASGSPIIDDNNNYVGIY", "aa_hexdigest": "bc2c400d684ad381fbedb897826651b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 38, "aa_cov": 0.7755102040816326, "hmm_cov": 1.0, "evalue": 9.653525971487587e-12, "score": 40.740234375, "start": 12, "stop": 49 } ], "seq_stats": { "molecular_weight": 5444.821500000002, "isoelectric_point": 5.4509389877319325 }, "id": "GPKLONFDEH_1085", "locus": "GCA000006625_01082" }, { "type": "cds", "sequence": "contig_1", "start": 641588, "stop": 641719, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTATCAATATCTAGTGCTGTAATTTTAATTATTTCTTTATTAATGTTTACGATTGTCTACTACAAGTATTTAAATAAAAACGATTATAAAGATAATCAAATTACTAATCAATATTCATTTAAATGA", "aa": "MPLSISSAVILIISLLMFTIVYYKYLNKNDYKDNQITNQYSFK", "aa_hexdigest": "76eaa0872b7fb290b79561f04429be36", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5094.936700000002, "isoelectric_point": 9.100760078430177 }, "id": "GPKLONFDEH_1086", "locus": "GCA000006625_01083" }, { "type": "cds", "sequence": "contig_1", "start": 641723, "stop": 641965, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAATTTCAGCAGAGGTAGTTAAAAAATTATTAATTGGTGATAATACATATATTCATTTAACAATATTTGCAATTTTTTACTCATTGTCATTAACATTAATGCGTTATCGTGATCTAATTGCTAAAAAGCAACAATCACCTTTTCCAACATTATTATTGCAAGTTTTAGTAATTGCTTTTGTTGTTGGTTTTGGTGTTGATTACCAAAGTAGTGTTAAGTTTGCTGGATTATTGGCGTGA", "aa": "MQISAEVVKKLLIGDNTYIHLTIFAIFYSLSLTLMRYRDLIAKKQQSPFPTLLLQVLVIAFVVGFGVDYQSSVKFAGLLA", "aa_hexdigest": "5b26f62b5ffb1b55a7a6b8d1b0a31163", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8967.622299999997, "isoelectric_point": 9.455400657653808 }, "id": "GPKLONFDEH_1087", "locus": "GCA000006625_01084" }, { "type": "cds", "sequence": "contig_1", "start": 642062, "stop": 642433, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTTATTCAAGGGATTGGATTAGGTTTTACTGATTACCTATTTTATATTTTTCAAAAACAAACTATTCGTGATCGTCGTTACCATTCGCGCCAATTATTTATTTGTGTTTTTGGTTTATTGTTTGTTTATTTAATGATTGCAGCAATTATTTTTGGTTTTTCAATCAAACCTCTATCAGTCGTTTATGCTAAAGAACAAAATGAAGCATATATTAATTTAGATGCAGAAATACCAGATCATTTTTATATGTCAATTCGTCAAGGAATTTTAAATAATCCTCGATTAGTATACTTTTTAGGGGCACAAGCCCATATTGATCCAAGATTGATTTTAGCACACTTAACAAACCATAATCCTGAGGTATGA", "aa": "MLFIQGIGLGFTDYLFYIFQKQTIRDRRYHSRQLFICVFGLLFVYLMIAAIIFGFSIKPLSVVYAKEQNEAYINLDAEIPDHFYMSIRQGILNNPRLVYFLGAQAHIDPRLILAHLTNHNPEV", "aa_hexdigest": "a0048af0d4973416a137d6b89546aa3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14370.663099999978, "isoelectric_point": 7.917313957214356 }, "id": "GPKLONFDEH_1088", "locus": "GCA000006625_01085" }, { "type": "cds", "sequence": "contig_1", "start": 642632, "stop": 642754, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAAGTTTTTTATTAGGTAAATATAGTATTTTAGCTTATAGTGGGTATGGTTTAGGATTGTCAATTGGTTATATTATTTCAACAATTGCCATTAGTATTATTAAATTTAGTAAAGATTGA", "aa": "MTSFLLGKYSILAYSGYGLGLSIGYIISTIAISIIKFSKD", "aa_hexdigest": "361525e7f541dafe8d163a9d112d133f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4306.066000000001, "isoelectric_point": 9.224861717224119 }, "id": "GPKLONFDEH_1089", "locus": "GCA000006625_01086" }, { "type": "cds", "sequence": "contig_1", "start": 643611, "stop": 643880, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATCCTTTTACACATCGATTTGTTGATATTTCAGCTTCAAATATTTATAGTTATTTAATTAAAAATAATGCTTATATTTTATTATCATTCTTTTGTACATTCTTTTCTTTTTCTTCTATTTTAAGTCGTTATGTAGGATTAATTGCCCGACGACAACGTCCTCCATATGTAATGATTGTTATTCAATTAGTTATGGTTGCGTTTACTTCTGGATTTGGGTTAACACACCAATCAGATCCACATTTCATTGGTTTAATGGCTTGA", "aa": "MKNPFTHRFVDISASNIYSYLIKNNAYILLSFFCTFFSFSSILSRYVGLIARRQRPPYVMIVIQLVMVAFTSGFGLTHQSDPHFIGLMA", "aa_hexdigest": "9043dbf0c57f3e119949083a14a08e28", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10218.919299999994, "isoelectric_point": 9.89423694610596 }, "id": "GPKLONFDEH_1090", "locus": "GCA000006625_01087" }, { "type": "cds", "sequence": "contig_1", "start": 643950, "stop": 644135, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATACAAGTGAACAAAGTCCATCTTATATATTTTTAGAAAAGCAAGTTCCCGAACATTTCTATCAATCTTTAAGAAAAATTTTAATTAATGATGAAAAATTATTAGATATTATTACTAGTCATGTGAATATTGGTCGCCAAGAAGTTGTTAATGCTTTATCATCAAATGATAAAAATATTTGA", "aa": "MYTSEQSPSYIFLEKQVPEHFYQSLRKILINDEKLLDIITSHVNIGRQEVVNALSSNDKNI", "aa_hexdigest": "75b59ca46c62d1be09a2042c2cd66623", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7107.961000000001, "isoelectric_point": 5.4973764419555655 }, "id": "GPKLONFDEH_1091", "locus": "GCA000006625_01088" }, { "type": "cds", "sequence": "contig_1", "start": 644211, "stop": 644420, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTTTGTTAATTACATTTGTGGTAATTAACTCACGTGTAGTTGGTTCATCATTATTAGATTTTCAATCAACGAGAATTCTAATGTACCAATCAATGATTTTCATACAAATGTTAAATTTAGGATTGTCAGATTTTTTATTTTATTTATATCAAAAACAAGAAGTCAGAGACTGTCGTTATCATTCAAGACAATTATTTTTTTGA", "aa": "MFILLITFVVINSRVVGSSLLDFQSTRILMYQSMIFIQMLNLGLSDFLFYLYQKQEVRDCRYHSRQLFF", "aa_hexdigest": "cb016cb9f81d4b455d57d835f32733ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8338.807799999997, "isoelectric_point": 9.032294654846194 }, "id": "GPKLONFDEH_1092", "locus": "GCA000006625_01089" }, { "type": "cds", "sequence": "contig_1", "start": 644658, "stop": 645248, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGAACAGAAACAAATGAAAGTTATTAAAAAATCCGATTCTTATTTTAAAAAAAATCTTACCGTTTTTAGAACACTTTTAGAACTTTTAAAAGAAAGCTCGCCATTTATCGCTGCAAGTGTTTTTACAGGACTATCATTAATTTTAATTGTTGTAATTGTTGCTCATCATAAAGATATTGGTGCTTATGCAGCAACTAGTATTAGTTATTTAACTATCTTTCAGTTAAGTTTTTTACAATTAGGAACAACCTTTGGCATTGTTTTTGCGATTTGGTCAAAAAGATCATTTAAAGATGGTAAGTATAAGTTTGAACCTACAAATGATACAGTAAATGCTGCTAGTTTTTATTCATTTATCTTTGGTTTAGTAGTTTTATTAATTTATTTTACAACCTCTTACATTTATAATCATTTTGCAAATGATCACCAAAATACAGTGATTAATCAAAGTGTAGGTAGTGCATATATTGTTTCAGGATTAGCAGTTGTTGGTTTAGCACCTTTGCGAAACTATTTATTGATGTTAATAAGATCAACCAAGTCCATTAATGTTTTATTAGCAATTGTTTTAGATTTTTGTACATGA", "aa": "MEEQKQMKVIKKSDSYFKKNLTVFRTLLELLKESSPFIAASVFTGLSLILIVVIVAHHKDIGAYAATSISYLTIFQLSFLQLGTTFGIVFAIWSKRSFKDGKYKFEPTNDTVNAASFYSFIFGLVVLLIYFTTSYIYNHFANDHQNTVINQSVGSAYIVSGLAVVGLAPLRNYLLMLIRSTKSINVLLAIVLDFCT", "aa_hexdigest": "10f5d74d56254ae19116c34b0f0234d2", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21966.469200000014, "isoelectric_point": 9.361599159240722 }, "id": "GPKLONFDEH_1093", "locus": "GCA000006625_01090" }, { "type": "cds", "sequence": "contig_1", "start": 645752, "stop": 646105, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAATAATAATAAAATTTCTGGAGCATATTCTATAAGTAACACTAAAGGCTTAGATAAAATTTTTATTTTAATGAATTATGATGAAACTTATAATTCTATTTTAACATTAGTTCATGAATTAGGGCATTCTGTACACACTTATTTTGCTAATCAATCACAAGAAGTATATAATGAATATGAAACCTTTTATGCAGAAATTGCTTCCATTACTAATGAGATCTTAATGAATTACCATTTATTAAAGAAATACGAAAATGATGATTTAATGCGTCTTTATATTTTAGATGAAATGATTAGTGGTTTTATTGCTACAACTACACGCCAAGCGATTTTTTCAAATTTTGAGTGA", "aa": "MPNNNKISGAYSISNTKGLDKIFILMNYDETYNSILTLVHELGHSVHTYFANQSQEVYNEYETFYAEIASITNEILMNYHLLKKYENDDLMRLYILDEMISGFIATTTRQAIFSNFE", "aa_hexdigest": "e2b613b70d7bf8bd1c67206ae87f0d46", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13656.192399999985, "isoelectric_point": 4.807919502258301 }, "id": "GPKLONFDEH_1094", "locus": "GCA000006625_01091" }, { "type": "cds", "sequence": "contig_1", "start": 646115, "stop": 646216, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTATTGAATCTGCTAAAAACTTAACTTTAGAAGCTTTGGCATTATTAGGTAGTGAATATATAAATGTTGTACAAAGAGCGTTCAATGAGCAATGA", "aa": "MFSIESAKNLTLEALALLGSEYINVVQRAFNEQ", "aa_hexdigest": "95632836096c4ae27ffc5bc86964ba95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3700.176200000001, "isoelectric_point": 4.493145179748535 }, "id": "GPKLONFDEH_1095", "locus": "GCA000006625_01092" }, { "type": "cds", "sequence": "contig_1", "start": 646250, "stop": 646630, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGTATCAGGATGGTATAAACTATAAAAAACAAAAAGTTTGTTTTAAAAATCAAACTGATCTTTATGTTGCATCTAAATCAAATGATCGTGCTTTACGAAAATCAGCTTATGAATCTCATTTTAAAGCGATATATGATTTACGTAATACTTTTAGTAAATTATTATATTATGAATATGTTAAACAAAACGAGTTAGCAAAATTACATAATTTTAAGGATTATATTTCTGCGGATGCTTTTAGTGATAAGGTTGATAAAAATTTTATAAATCATATTTATACACAAACAAAAAAATTTGCTAAAGGAATTAATAGATATACAAAATATCGAACATTATTTCTAAAACAACAATATCAATTAACTAAAGTTGAACCATGA", "aa": "MQYQDGINYKKQKVCFKNQTDLYVASKSNDRALRKSAYESHFKAIYDLRNTFSKLLYYEYVKQNELAKLHNFKDYISADAFSDKVDKNFINHIYTQTKKFAKGINRYTKYRTLFLKQQYQLTKVEP", "aa_hexdigest": "43a98bfe440b008153b9039c291b589d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15199.143699999977, "isoelectric_point": 9.667437171936037 }, "id": "GPKLONFDEH_1096", "locus": "GCA000006625_01093" }, { "type": "cds", "sequence": "contig_1", "start": 646739, "stop": 647074, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCAATGAATTTGTTGAATCAAATAATGAATTAATAAAAGCTTTTGAAAAGTGGATTAATAGTTTAAATGATTTTATTAATTTTCACGAAGTTTCAGAAAAAAATTTATTAATTAGTAATCGAATTAACAATTATGTTTCAAATAAATTGCAAACAAATTTAATCGATAATGAAATGCTTGCTTGGTCTCAAAAAATTGAACATGAACAACATCGTGTAGCTAAAATTTTTATTAATTTTGAAAATTTAGCAATAAAAAATAAAGATTTAATTAATTCATATTTAAAAAATTCTTCATTAATTAAATACCAATTAGAATATGAAGAATTATGA", "aa": "MFNEFVESNNELIKAFEKWINSLNDFINFHEVSEKNLLISNRINNYVSNKLQTNLIDNEMLAWSQKIEHEQHRVAKIFINFENLAIKNKDLINSYLKNSSLIKYQLEYEEL", "aa_hexdigest": "2c45206020c139e548801ff105587d80", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13325.931799999988, "isoelectric_point": 5.354596900939941 }, "id": "GPKLONFDEH_1097", "locus": "GCA000006625_01094" }, { "type": "cds", "sequence": "contig_1", "start": 647241, "stop": 647645, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03144.31", "PFAM:PF03143.23" ], "nt": "ATGTTTAGAAAATCATTAGATCAAGCTGAAGCTGGTGATAATGCTGGTATTTTATTACGTGGTATTAAAAAAGAAGATGTTGAACGTGGTCAAGTACTTGTAAAACCAGGATCAATTAAACCTCACCGTACTTTTACTGCTAAAGTTTATATTCTTAAAAAAGAAGAAGGTGGACGTCATACACCTATTGTTTCAGGATACCGTCCACAATTCTATTTTAGAACAACAGATGTAACAGGTGCTATTTCATTACCTGCTGGTGTTGATTTGGTTATGCCAGGTGATGACGTTGAAATGACTGTAGAATTAATTGCTCCAGTTGCGATTGAAGATGGATCTAAATTCTCAATCCGTGAAGGTGGTAAAACTGTAGGTCATGGTAGCGTAATTAAAACAAGTAATTAA", "aa": "MFRKSLDQAEAGDNAGILLRGIKKEDVERGQVLVKPGSIKPHRTFTAKVYILKKEEGGRHTPIVSGYRPQFYFRTTDVTGAISLPAGVDLVMPGDDVEMTVELIAPVAIEDGSKFSIREGGKTVGHGSVIKTSN", "aa_hexdigest": "b9d779149b8642ada7b9e4f029200e83", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001EFCDF7C", "query_cov": 0.9776119402985075, "subject_cov": 0.8136645962732919, "identity": 0.71, "score": 201.0, "evalue": 5.59e-64, "valid": false, "product": "EF-Tu/IF-2/RF-3 family GTPase", "uniref50_id": "UniRef50_A0A966IFR0" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03144.31", "name": "GTP_EFTU_D2", "length": 33, "aa_cov": 0.2462686567164179, "hmm_cov": 1.0, "evalue": 3.0706026052296705e-09, "score": 32.804351806640625, "start": 1, "stop": 33 }, { "id": "PF03143.23", "name": "GTP_EFTU_D3", "length": 104, "aa_cov": 0.7014925373134329, "hmm_cov": 1.0, "evalue": 3.135629141787503e-39, "score": 129.16432189941406, "start": 38, "stop": 131 } ], "seq_stats": { "molecular_weight": 14496.45239999999, "isoelectric_point": 8.89097995758057 }, "id": "GPKLONFDEH_1098", "locus": "GCA000006625_01095" }, { "type": "cds", "sequence": "contig_1", "start": 647674, "stop": 648054, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTTTTAGACCAACAATTTCAACCTCATCATTAACTTTTAATACACCACGTTCAACACGTCCAGTTACTACTGTACCACGTCCTGAAATTGTGAATACATCTTCAATTGCTAATAAGAATGGTTTGTCAGTACTACGTTCTGGTAATGGAATTCATGAATCAACTGCGTCCATTAATTCATCAATTTTTGCTTCTCAAACTGGATCTCCTTCTAAAGCCTTAAGACCTGAACCACGAATAACTGGTGTGTTATCGCCATCAAATCCATATTTAGATAATAATTCACGAACTTCCATTTCAACAAGATCTTGCATATCTGGATCTGTCATGAAATCACATTTGTTTAAGAAAACAACGATTTTTGGAACACCAACTTGA", "aa": "MSFRPTISTSSLTFNTPRSTRPVTTVPRPEIVNTSSIANKNGLSVLRSGNGIHESTASINSSIFASQTGSPSKALRPEPRITGVLSPSNPYLDNNSRTSISTRSCISGSVMKSHLFKKTTIFGTPT", "aa_hexdigest": "656585c553c3dfd335afe55298d7705b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13426.987699999976, "isoelectric_point": 11.505882072448731 }, "id": "GPKLONFDEH_1099", "locus": "GCA000006625_01096" }, { "type": "cds", "sequence": "contig_1", "start": 647871, "stop": 648425, "strand": "-", "frame": 2, "gene": null, "product": "Elongation factor Tu", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0G0Z5D4" ], "nt": "ATGGCTAAAGCTAAATTTGAAAGAACAAAACCTCACGTTAACATTGGTACTATAGGCCACGTTGACCATGGTAAAACTACTTTAACAGCTGCAATTAGTACTGTACTTGCCAAAAAAGGACAAGCTATTGCACAATCATATGCTGATGTTGATAAAACACCAGAAGAAAGAGAACGTGGTATTACAATTAACGCTTCTCACGTTGAATATGAAACAAAAACACGTCACTATGCACACGTTGACTGTCCAGGACATGCCGATTATGTAAAAAATATGATTACAGGGGCAGCACAAATGGATGGAGCAATTTTAGTTATTGCTGCATCTGATGGGGTTATGGCTCAAACTAAAGAACATATTTTATTAGCACGTCAAGTTGGTGTTCCAAAAATCGTTGTTTTCTTAAACAAATGTGATTTCATGACAGATCCAGATATGCAAGATCTTGTTGAAATGGAAGTTCGTGAATTATTATCTAAATATGGATTTGATGGCGATAACACACCAGTTATTCGTGGTTCAGGTCTTAAGGCTTTAGAAGGAGATCCAGTTTGA", "aa": "MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAISTVLAKKGQAIAQSYADVDKTPEERERGITINASHVEYETKTRHYAHVDCPGHADYVKNMITGAAQMDGAILVIAASDGVMAQTKEHILLARQVGVPKIVVFLNKCDFMTDPDMQDLVEMEVRELLSKYGFDGDNTPVIRGSGLKALEGDPV", "aa_hexdigest": "5766f16942088d67ef841c651a1648d5", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002A75969B", "query_cov": 0.9945652173913043, "subject_cov": 0.8883495145631068, "identity": 0.7809999999999999, "score": 294.0, "evalue": 1.86e-99, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A0G0Z5D4" }, "pscc": { "uniref50_id": "UniRef50_A0A0G0Z5D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0G0Z5D4" ], "product": "Elongation factor Tu" }, "genes": [], "id": "GPKLONFDEH_1100", "locus": "GCA000006625_01097" }, { "type": "cds", "sequence": "contig_1", "start": 648562, "stop": 649011, "strand": "-", "frame": 1, "gene": null, "product": "Translation elongation factor G, elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F9ZJJ1" ], "nt": "TTGATTGAGGCATTAACATCAGGGCCAATTGCTGGTTATCAAACAATTGACGTTAAAGCAACTATTTTTGATGGTTCATATCATGATGTTGATTCATCTGGTATGGCTTATAAAATTGCTGCATCTCTTGCTTTTAAAGAAGCAGCAAAAGTATGCAAACCAGTTTTATTAGAACCTATTATGTCAGTTGATGTAACTACACCTGATGATTATTTTGGGACGGTAATGGGCGATATTTCAAAACGTCGTGGTGTAATTGAAGGACAAGAACAACGTGGTAATGCACAAGCTATTAAAGCTAAAGTTCCGCTATCAGAAATGTTTGGATATGCAACGGATTTACGTTCAAATACACAAGGACGTGGTCAATATATTATGCAATTCAGTCACTATGCACAAGCACCTAAGTCTGTTACTGAAGAAGTAATGGTAGCACGTGCAAAAAAATAA", "aa": "MIEALTSGPIAGYQTIDVKATIFDGSYHDVDSSGMAYKIAASLAFKEAAKVCKPVLLEPIMSVDVTTPDDYFGTVMGDISKRRGVIEGQEQRGNAQAIKAKVPLSEMFGYATDLRSNTQGRGQYIMQFSHYAQAPKSVTEEVMVARAKK", "aa_hexdigest": "d91232521edf4792bd164ecb0ea220ff", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A0F9ZJJ1", "query_cov": 0.9865771812080537, "subject_cov": 0.8855421686746988, "identity": 0.605, "score": 181.0, "evalue": 4.79e-56, "valid": false, "product": "Translation elongation factor G, elongation factor G", "uniref50_id": "UniRef50_A0A0F9ZJJ1" }, "pscc": { "uniref50_id": "UniRef50_A0A0F9ZJJ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F9ZJJ1" ], "product": "Translation elongation factor G, elongation factor G" }, "genes": [], "id": "GPKLONFDEH_1101", "locus": "GCA000006625_01098" }, { "type": "cds", "sequence": "contig_1", "start": 649111, "stop": 650226, "strand": "-", "frame": 1, "gene": null, "product": "GTP dependent elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A346FYQ4" ], "nt": "ATGGATAAAACAGGTGCTGATTTTTATTACTCAATTGAGACAATGAAGAATCGTTTAGGTGTTAAAGCCACAGCTATTCAAATTCCAATTGGTGCTGAAGCCGATTTTGTTGGAAGTATTGATTTAATTGAAATGAAAGCTTATATTTATGATGGAAAAGCTGATGAAGAATATAAAATTGAAGATATTCCAGCTGATTACTTAACAAAAGCACAAGTAATGCGTTCACAAATGATCGATGATGTAGCTGTTTTTGATGATGAAGTGATGGAAAAATACTTATCTGGTGAAGAACTAAGTCATGAAGATATTAAAAAATGTATTCGTAAAGGAGTAATTTCAACTGAATTATACCCAGTATTATGTGGTACAGCTTTTAAAAACAAAGGTGTTAAAAAATTATTAGACGCAGTTGTTGATTTCTTACCTTCACCAATTGATGTTCCACCAATTAAAGGTGTTGATGATCATGGTAATCCAATTGAATATCATAATGATCCAAATGAACCATTTGCTGCCCTTGCCTTCAAAGTCGCAACTGATCCTTTTGTTGGACGTTTAACATATATTCGTGTATATAGTGGTAAACTTGACAAAGGTACTTATATTTACAATGCAACAAAAGATAAAAAAGAAAGAATTTCTCGTCTTGTAAAAATGCATTCAAACAACCGTGATGAGATTGATTCAATTAGTGCTGGTGATATTTGTGCTGTAATTGGATTAAAAGACACTACTACTGGTGATACAATTTGTGATGAAAAAAAACCAGTTATTTTAGAACAAATGGTATTTGCAGAACCTGTTATTTCTTTATCTGTTGAACCAAAAACTAAAGCAGATCAAGAAAAGATGAGTTTAGCTTTATCTAAATTAGCTGAAGAAGATCCAACTTTTAGAACATACACAAATGAAGAAACAGGTCAAACTATTATTGCTGGAATGGGTGAATTACATTTAGATGTTTTGGTTGATCGTATGCGTCGTGAATTTAATGTTCAAGTTAATGTAGGTGCTCCTCAAGTTAGTTATCGTGAGACATTTACTGAAGTTGCTGATGCTGAAGGTAAGTATATTAAGCAATCTGGTGGACGTGGTCAATATGGGCATGTCTGA", "aa": "MDKTGADFYYSIETMKNRLGVKATAIQIPIGAEADFVGSIDLIEMKAYIYDGKADEEYKIEDIPADYLTKAQVMRSQMIDDVAVFDDEVMEKYLSGEELSHEDIKKCIRKGVISTELYPVLCGTAFKNKGVKKLLDAVVDFLPSPIDVPPIKGVDDHGNPIEYHNDPNEPFAALAFKVATDPFVGRLTYIRVYSGKLDKGTYIYNATKDKKERISRLVKMHSNNRDEIDSISAGDICAVIGLKDTTTGDTICDEKKPVILEQMVFAEPVISLSVEPKTKADQEKMSLALSKLAEEDPTFRTYTNEETGQTIIAGMGELHLDVLVDRMRREFNVQVNVGAPQVSYRETFTEVADAEGKYIKQSGGRGQYGHV", "aa_hexdigest": "6c002e8bbb22673bb1295ca0dd903080", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000DB1047A", "query_cov": 0.9622641509433962, "subject_cov": 0.8283062645011601, "identity": 0.555, "score": 395.0, "evalue": 1.12e-132, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A346FYQ4", "kegg_orthology_id": "K02355" }, "pscc": { "uniref50_id": "UniRef50_A0A346FYQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A346FYQ4" ], "product": "GTP dependent elongation factor G" }, "genes": [], "id": "GPKLONFDEH_1102", "locus": "GCA000006625_01099" }, { "type": "cds", "sequence": "contig_1", "start": 650479, "stop": 650628, "strand": "-", "frame": 1, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D1ZAS3", "UniRef:UniRef90_UPI002E75BBB3" ], "nt": "ATGTCTAAAGAATTAAAATTATTTCGTAACTTTGGAATCATGGCACACATTGATGCTGGTAAAACAACAACCTCTGAACGGATTTTATATCATACAGGAAAGAATCATAAAATCGGAGAGACTCACGATGGTGCTGCAACAATGGATTGA", "aa": "MSKELKLFRNFGIMAHIDAGKTTTSERILYHTGKNHKIGETHDGAATMD", "aa_hexdigest": "9cc34fff7773c9f9f56edc1c04410a7b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0025E4693E", "query_cov": 0.9183673469387755, "subject_cov": 0.8181818181818182, "identity": 0.8220000000000001, "score": 80.5, "evalue": 3.7e-19, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A292SDT6" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 650380, "stop": 650625, "observations": { "mutation": [ 650421, 650481 ], "stop": [ 650421, 650481 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002E75BBB3", "query_cov": 0.9795918367346939, "subject_cov": 0.5161290322580645, "identity": 0.833, "score": 87.4, "evalue": 1.8e-21, "gene_start": 2, "gene_end": 49, "reference_start": 4, "reference_end": 51, "reference_sequence": "MAKTNDLKLTRNFGIMAHIDAGKTTTTERILYYTGKTHKIGEVHDGAATMDWMVQEQERGVTITAAATTCFWKYPGGADNGKEYRFQIIDTPG" }, "paralog": false, "identity": 0.6444444444444445, "score": 112.0, "evalue": 3.05e-34, "description": "Internal stop codon at 650421, 650481. Nonsense mutation around 650421, 650481.", "psc": { "uniref90_id": "UniRef90_UPI002E75BBB3", "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A3D1ZAS3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002E75BBB3", "UniRef:UniRef50_A0A3D1ZAS3" ] } }, "id": "GPKLONFDEH_1103", "locus": "GCA000006625_01100" }, { "type": "cds", "sequence": "contig_1", "start": 650658, "stop": 650780, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00177.27" ], "nt": "ATGATTGAGAAATTAGCACATGAAATTATTGATGCTTCAAATAATGTAGGAGCAGCAATTAAGAAAAAAGAAGATACTCATAAAATGGCAGAAGCAAATAAAGCTTTTGCACACCTACGTTGA", "aa": "MIEKLAHEIIDASNNVGAAIKKKEDTHKMAEANKAFAHLR", "aa_hexdigest": "28c15e008dc86b5208bec9f4df89b165", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.75, "score": 64.7, "evalue": 4.05e-13, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00177.27", "name": "Ribosomal_S7", "length": 34, "aa_cov": 0.85, "hmm_cov": 1.0, "evalue": 1.1215280975113744e-14, "score": 49.83702850341797, "start": 1, "stop": 34 } ], "seq_stats": { "molecular_weight": 4445.0894, "isoelectric_point": 8.15926380157471 }, "id": "GPKLONFDEH_1104", "locus": "GCA000006625_01101" }, { "type": "cds", "sequence": "contig_1", "start": 650817, "stop": 651122, "strand": "-", "frame": 2, "gene": "rpsG", "product": "30S ribosomal protein S7", "db_xrefs": [ "COG:COG0049", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02992", "SO:0001217", "UniRef:UniRef50_P02359", "UniRef:UniRef90_Q9PPW6" ], "nt": "ATGAGAAAATTAAAACCACAAAAAAGACAAGTTTTAGCTGATCCAGTATATAACTCTCGTCTTGTAACTAAACTAATCAATGCTATTATGTATGATGGTAAAAAAGGATTAGCACAATCTATTATTTATTCAGCCTTTGAGATTGTTGAACAAAAGACTGGCAAACCAGCTTTAGAAGTTTTTAATAAGGCGATTGATAATGTAATGCCAATTATTGAACTTAAAGTAAGACGTGTTGGCGGTTCAAACTTTCAAGTACCAACTGAAGTAACGCCAGAACGTCGTCAGACTTTAGGTTTAAGATGA", "aa": "MRKLKPQKRQVLADPVYNSRLVTKLINAIMYDGKKGLAQSIIYSAFEIVEQKTGKPALEVFNKAIDNVMPIIELKVRRVGGSNFQVPTEVTPERRQTLGLR", "aa_hexdigest": "1f53bde9036a0009f1d11af9f19cc737", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A519VYV2", "query_cov": 1.0, "subject_cov": 0.8278688524590164, "identity": 0.574, "score": 123.0, "evalue": 2.44e-34, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A4Q7IH49", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "genes": [ "rpsG" ], "truncated": "5-prime", "pseudogene": { "start": 650658, "stop": 651122, "observations": { "mutation": [ 650819 ], "stop": [ 650819 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPW6", "query_cov": 1.0, "subject_cov": 0.6516129032258065, "identity": 1.0, "score": 196.0, "evalue": 5.67e-63, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MRKLKPQKRQVLADPVYNSRLVTKLINAIMYDGKKGLAQSIIYSAFEIVEQKTGKPALEVFNKAIDNVMPIIELKVRRVGGSNFQVPTEVTPERRQTLGLRWITLYARLRHEHTMIEKLAHEIIDASNNVGAAIKKKEDTHKMAEANKAFAHLRW" }, "paralog": false, "identity": 0.9935064935064936, "score": 298.0, "evalue": 1.5e-105, "description": "Internal stop codon at 650819. Nonsense mutation around 650819.", "psc": { "uniref90_id": "UniRef90_Q9PPW6", "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_P02359", "cog_id": "COG0049", "cog_category": "J", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPW6", "UniRef:UniRef50_P02359", "COG:COG0049", "COG:J", "KEGG:K02992", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P02359", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P02359" ], "product": "Small ribosomal subunit protein uS7" } }, "id": "GPKLONFDEH_1105", "locus": "GCA000006625_01102" }, { "type": "cds", "sequence": "contig_1", "start": 651150, "stop": 651575, "strand": "-", "frame": 2, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_004026220.1", "SO:0001217", "UniParc:UPI0000134C86", "UniRef:UniRef100_Q9PPW5", "UniRef:UniRef50_P0A0H0", "UniRef:UniRef90_Q9PPW5" ], "nt": "ATGCCTACAATTGCGCAATTGATTCGTAATAAACGAGCACCTAAAGTAAAAAAAACTAAATCACCTGCTCTTTTATTTACATACAACTCATTACATAAAAAAACAACAAAAAACCCATCACCTCTAAAATCTGGTGTTTGTACTAGAGTTGGTACAATGACACCTAAAAAACCTAACTCAGCTTTACGTAAATATGCTAAAGTTCGTTTAAGTAATGGTTTTGAAGTTTTAGCTTACATTCCAGGTGAAGGACATAACTTACAAGAACACAGTGTCGTAGTTATTCGTGGGGGAAGAGTTAAAGACTTACCAGGGGTTCGTTATCATATTGTTCGTGGTGCTGGCGACGCATCAGGTGTTGAAAAACGTCGTCAACAACGTTCACTATACGGTGCTAAACGTCCTAAAAAAGAAGCATCTAAATAA", "aa": "MPTIAQLIRNKRAPKVKKTKSPALLFTYNSLHKKTTKNPSPLKSGVCTRVGTMTPKKPNSALRKYAKVRLSNGFEVLAYIPGEGHNLQEHSVVVIRGGRVKDLPGVRYHIVRGAGDASGVEKRRQQRSLYGAKRPKKEASK", "aa_hexdigest": "f39210c6f128ba87d0c2b122ab4ae94f", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000134C86", "ncbi_nrp_id": "WP_004026220.1", "uniref100_id": "UniRef100_Q9PPW5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134C86", "RefSeq:WP_004026220.1", "UniRef:UniRef100_Q9PPW5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPW5", "uniref90_id": "UniRef90_Q9PPW5" }, "psc": { "uniref90_id": "UniRef90_Q9PPW5", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P0A0H0", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P0A0H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A0H0" ], "product": "Small ribosomal subunit protein uS12" }, "genes": [ "rpsL" ], "id": "GPKLONFDEH_1106", "locus": "GCA000006625_01103" }, { "type": "cds", "sequence": "contig_1", "start": 651914, "stop": 652177, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTATGAATAAGCGTACACAAGGTTATGATTTAAGTGAATTCGGTAGTGGTAATGCTGTAGTTTTAAAAACTGTAATGCGTATTGGTGATAATTTTGATGACTTTAATGATAATGCTTCAAAAGGTAAACTTAATAGTGAACGTGTAGCATTATTAAAAGAATATGGTAAATTATTTGGTAATTTTGATATAAATAATAAAGGTGTTAAATACGAAAAAAATCAAAACGGTCAAATGGTGAAATCTGATGAAACTTGA", "aa": "MNAMNKRTQGYDLSEFGSGNAVVLKTVMRIGDNFDDFNDNASKGKLNSERVALLKEYGKLFGNFDINNKGVKYEKNQNGQMVKSDET", "aa_hexdigest": "90fc472edbd6ca7b73cd4d88a0070be1", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9759.786899999996, "isoelectric_point": 7.925308036804201 }, "id": "GPKLONFDEH_1107", "locus": "GCA000006625_01104" }, { "type": "cds", "sequence": "contig_1", "start": 652280, "stop": 652375, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAACTCAAACCGTGAACAAGAAAACCAAGTCGATCCAACTATTGAAAACTTGGTTAGTGAAGGCTTAGAACGTAGTTTATTACCTAAATGA", "aa": "MFNSNREQENQVDPTIENLVSEGLERSLLPK", "aa_hexdigest": "e247dbb06042c7c4c6acca14559dd23e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3587.9225000000006, "isoelectric_point": 4.420448112487794 }, "id": "GPKLONFDEH_1108", "locus": "GCA000006625_01105" }, { "type": "cds", "sequence": "contig_1", "start": 652463, "stop": 652714, "strand": "-", "frame": 3, "gene": null, "product": "HAD family acid phosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E042A4" ], "nt": "ATGCTATTAACAGAGTATAATAGTGCAAAACACCAATTTGATGCAATGATTAAACAAAGTGATTTTGATACAAGTAAAGTAAAAGTAGAAAAAGATTCAAATGGTAATATTACAAAGGTAACAGTTTCTAATCCTGAAAGTAATAAGGCAATTCCTGTAGTATTTATGGATATTGATGAAACAATTTTAAATAATTACGCTAATCAAAACTATCAATTATTAAATAATAAAGCTTATAATCCAAAGGATTGA", "aa": "MLLTEYNSAKHQFDAMIKQSDFDTSKVKVEKDSNGNITKVTVSNPESNKAIPVVFMDIDETILNNYANQNYQLLNNKAYNPKD", "aa_hexdigest": "63ac6f670ddc86367703f3c44d7a176d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021E042A4", "query_cov": 0.8795180722891566, "subject_cov": 1.0579710144927537, "identity": 0.603, "score": 72.8, "evalue": 2.34e-15, "valid": false, "product": "HAD family acid phosphatase", "uniref50_id": "UniRef50_UPI0021E042A4" }, "pscc": { "uniref50_id": "UniRef50_UPI0021E042A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E042A4" ], "product": "HAD family acid phosphatase" }, "genes": [], "id": "GPKLONFDEH_1109", "locus": "GCA000006625_01106" }, { "type": "cds", "sequence": "contig_1", "start": 653495, "stop": 653602, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTAAAATTAAGTGATAAAGAAGAACTTCAACCAGAACAAATTAAAAAACGAACTCAAGCTATTGCGTTGGTATTATCAATTGCTTTATTTAATGAAGATTGA", "aa": "MSLKLSDKEELQPEQIKKRTQAIALVLSIALFNED", "aa_hexdigest": "c8f3fd2406659f0aa89d80f0c82ca54e", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4000.6151, "isoelectric_point": 5.149635505676269 }, "id": "GPKLONFDEH_1110", "locus": "GCA000006625_01107" }, { "type": "cds", "sequence": "contig_1", "start": 653723, "stop": 654526, "strand": "-", "frame": 3, "gene": null, "product": "HNH endonuclease family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E6518D7", "UniRef:UniRef90_UPI001E6518D7" ], "nt": "TTGAAATATTTTAGTAAATATATAGATATTTACAATGACTTAACAATCAAAGATAAATTCACAAACAATAATTGTAATTTTTATTTAATTAGTGATTATTTACTAATCTTAACGAACAAAAAGAAAAAGAATTATATTCCTTTATTAGTTTATTTATTAGAGAAAATATTTACTGAGGATGTATTGTATGATTTTGATAACAATGGTAATTTTGATAAAAGCAAATTAAGAATTAATATTGAAGAATATGTTAGCGTGATTGCTAATTTTTCATTGAGAAATGTTTTATTAAATACTCGTGGTCAAAGTATTTTAAATAGTGTTATTAAATTAATTAAGTTAATTGATAATGATATTAACAAAAACAATATAAATTCCATTAAAAATATTAATTTATCAAAAAAATTTTTAGATGTTTTTAATAGCAATAATCAAATGGAAATAAAAAATATTAAAAATTTTTATAACAAAATGCTTGATGAAAAGGTTAGTGATATTCATTTGGCTTATTTAATTAGATTTGAAACTTATTTAACTTTTTTTCTTGATGATACGCAAATAAATAAAAATAAGGATACAAAAGTATTTTTTTATCCACCAAGTAATGCTGATAATGAAAAAGCAGATTTAACAATTGAACACATGTATTCACAATCATTAAAAGATGAAAGATTTAATATTGTTAAAAATAATATTGGTAATTTAATACCACTTACTAAAGAATACAACTCAGCTCTTAATAATAAAGAATTTAAAGATAAAAAACAACGTTATAAAAATGCACCTATCATTCAAAATCTTTGA", "aa": "MKYFSKYIDIYNDLTIKDKFTNNNCNFYLISDYLLILTNKKKKNYIPLLVYLLEKIFTEDVLYDFDNNGNFDKSKLRINIEEYVSVIANFSLRNVLLNTRGQSILNSVIKLIKLIDNDINKNNINSIKNINLSKKFLDVFNSNNQMEIKNIKNFYNKMLDEKVSDIHLAYLIRFETYLTFFLDDTQINKNKDTKVFFYPPSNADNEKADLTIEHMYSQSLKDERFNIVKNNIGNLIPLTKEYNSALNNKEFKDKKQRYKNAPIIQNL", "aa_hexdigest": "a45267757a4132ffa840b6dfae2303c9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 653483, "stop": 654712, "observations": { "mutation": [ 653497, 653725, 654538, 654589 ], "stop": [ 653497, 653725, 654538, 654589 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E6518D7", "query_cov": 1.0, "subject_cov": 0.6480582524271845, "identity": 0.996, "score": 514.0, "evalue": 6.06e-182, "gene_start": 1, "gene_end": 267, "reference_start": 66, "reference_end": 332, "reference_sequence": "MKIIIEVKRFNEFVDSLSALNLCKDEDLKRFEKNDKDDEYYNKSWFFIIEIINKKFTLKNNWKACLKYFSKYIDIYNDLTIKDKFTNNNCNFYLISDYLLILTNKKKKNYIPLLVYLLEKIFTEDVLYDFDNNGNFDKSKLRINIEEYVSVIANFSLRNVLLNTRGQSILNSVIKLIKLIDNDINKNNINSIKNINLSKKFLDVFNSNNQMEIKNIKNFYNKMLDEKVSDIHLAYLIRFETYLTFFLDDTQINKNKDTKVFFYPPSNADNEKADLTIEHMYSQSLKDERFNIVKNNIGNLIPLTKEYNSALNNKEFKDKKQRYKNAPIIQNLWLYKGLNEEQITKLYNEFTKINEKLKSNKIDVNEDVFKEALMSLKLSDKEELQPEQIKKRTQAIALVLSIALFNEDWDVE" }, "paralog": false, "identity": 0.9902200488997555, "score": 796.0, "evalue": 1.54e-295, "description": "Internal stop codon at 653497, 653725, 654538, 654589. Nonsense mutation around 653497, 653725, 654538, 654589.", "psc": { "uniref90_id": "UniRef90_UPI001E6518D7", "product": "HNH endonuclease family protein", "uniref50_id": "UniRef50_UPI001E6518D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E6518D7", "UniRef:UniRef50_UPI001E6518D7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E6518D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E6518D7" ], "product": "HNH endonuclease family protein" } }, "id": "GPKLONFDEH_1111", "locus": "GCA000006625_01108" }, { "type": "cds", "sequence": "contig_1", "start": 654995, "stop": 655387, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03235.19" ], "nt": "TTGCTAGAAGATATTAATGATATTAGTAACAAACAAAATAATGAAAACCATTTTTTAGGTAGTATCATTAGTGTCTTAGTTAAAGAAGAATCTATTGATAATATTCAAATTATTGATGGTCAACAACGATTAACAACCTTATTTTTAATTACAAAAGCATTTATTATTTTCTTGTATAACAACAGAGAAGAAATAACAAAATTTACAAACGAACAAATAAAGCAACTAAGTCATTTAAAAGATATCTTTTTTAACAGAGAGTTTATAATTGATCGCTTTAGTCGTATTGGTGGCAATCCTGACTATGAAGCTTTAAAACAAATATTATCTAATGATGAATTAGATAATAAAAACAATCATATTTTTATGAATCTAAAAGATATTGTTGAATGA", "aa": "MLEDINDISNKQNNENHFLGSIISVLVKEESIDNIQIIDGQQRLTTLFLITKAFIIFLYNNREEITKFTNEQIKQLSHLKDIFFNREFIIDRFSRIGGNPDYEALKQILSNDELDNKNNHIFMNLKDIVE", "aa_hexdigest": "baed8c4e96df2a5d264c48de58660cce", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03235.19", "name": "GmrSD_N", "length": 129, "aa_cov": 0.9692307692307692, "hmm_cov": 0.9147286821705426, "evalue": 7.163603215410037e-15, "score": 51.205928802490234, "start": 3, "stop": 128 } ], "seq_stats": { "molecular_weight": 15332.19339999998, "isoelectric_point": 4.935466194152831 }, "id": "GPKLONFDEH_1112", "locus": "GCA000006625_01109" }, { "type": "cds", "sequence": "contig_1", "start": 656402, "stop": 656545, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCTTTAATGAAAAAGATTTTGAAGCGATTCAAGCTAAGGGAATATCAAAAGCTAAAATGTATCAACAAGCTGGAAATTCAATTGTTGTTGATGTAATGGTTTATTTAATGAAATTAATTCAAGAAAGATTTAATAAATAA", "aa": "MGFNEKDFEAIQAKGISKAKMYQQAGNSIVVDVMVYLMKLIQERFNK", "aa_hexdigest": "1277fc9019016dfa4a458f067e2ece76", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5413.317699999999, "isoelectric_point": 9.15762119293213 }, "id": "GPKLONFDEH_1113", "locus": "GCA000006625_01110" }, { "type": "cds", "sequence": "contig_1", "start": 657146, "stop": 657301, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00145.23" ], "nt": "GTGACTGGTGAGCAGTTAATAAAAAGTGTTGGGAACAAAGGGATAGATTTACTTACTTATTCATTCCCTTGTCAAGACTTATCAACAGCTGGAAGCTTTAGTAAAAAAGCTTTTGGAATGAAAAAAGGATCCGGTACACGTTCTGGATTACTATGA", "aa": "MTGEQLIKSVGNKGIDLLTYSFPCQDLSTAGSFSKKAFGMKKGSGTRSGLL", "aa_hexdigest": "80162e50db3455a682498a1dc9571bcc", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00145.23", "name": "DNA_methylase", "length": 33, "aa_cov": 0.6470588235294118, "hmm_cov": 0.9090909090909091, "evalue": 3.2711742749722345e-07, "score": 25.599069595336914, "start": 11, "stop": 43 } ], "seq_stats": { "molecular_weight": 5357.144, "isoelectric_point": 9.655059242248537 }, "id": "GPKLONFDEH_1114", "locus": "GCA000006625_01111" }, { "type": "cds", "sequence": "contig_1", "start": 657864, "stop": 658127, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTTATAAATTATTTAAAAAATGTAAAAGGATATAGTGATGGTACAATTGCTATTTATCAAAAATATGTTAAAGTACTAATTGATAATAATTTAGATTATTTAAAAGCTATTAAAAAATATGAACATAACGCTAATGTTACTAAGCGCGTTGTTTTAAGTGCTTTTAAAGCATATTTAAAATTTATTAATCATAAAGATGCTATATTGATTGTTATGCCTAAAAAAGAAATTAAAGTAAAAGAATTTTTGACTTGA", "aa": "MKKFINYLKNVKGYSDGTIAIYQKYVKVLIDNNLDYLKAIKKYEHNANVTKRVVLSAFKAYLKFINHKDAILIVMPKKEIKVKEFLT", "aa_hexdigest": "b96b5af5e50bad43d14466c6442fa225", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10227.125999999993, "isoelectric_point": 9.853492927551269 }, "id": "GPKLONFDEH_1115", "locus": "GCA000006625_01112" }, { "type": "cds", "sequence": "contig_1", "start": 658401, "stop": 658619, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATCAAAAAGTTACTAGAATTAAAACTAATATTAGGCTAACGCCTCATATTTTACGTCGTAGTTTTGCTAACCATTGCATTAATAATGGTATTAATATTTATGATTTATCATTAGTTATGGGTCATAACAATATTAATTCAACAAGTTTATATTTAAATAAGGAAAGTAAATTAGTAGAATTAGATCATCTTTTTGATAATAAAAAAGTTAATTAA", "aa": "MYQKVTRIKTNIRLTPHILRRSFANHCINNGINIYDLSLVMGHNNINSTSLYLNKESKLVELDHLFDNKKVN", "aa_hexdigest": "39f9d1d0259e638e4c73a8452b5e3af7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8425.642599999996, "isoelectric_point": 9.728746604919433 }, "id": "GPKLONFDEH_1116", "locus": "GCA000006625_01113" }, { "type": "cds", "sequence": "contig_1", "start": 659079, "stop": 659459, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "TTGACTGGTAATCAAACAGGTGTTTTAATTGCGCATTATTTATTAGAACATAAAAAATTTACTAAACCTACATATATTATTTCAACATTTATTTCAACACGCTATCCAGAATTATTTGCTAAAAATTTTGATTGTGATGTTTTATATGTAGATGTAGGTTTTAAAAATCATGGTAATTTAATTGCCAATCGTAAAAAAACACATAATTTGGTTGTTGGTTTTGAAGAAGCAATTGGTTCATTACCATCTGATATTAATAATGATAAAGATTCATATCAAACTGCGTCTATTTTGTTAGAAATGATTAATTATTATTATGAACAAAAACTTGATTTATTTACCGTTTTAAAAAAAGAGATTTTTGCTAAGTATGGGAATTGA", "aa": "MTGNQTGVLIAHYLLEHKKFTKPTYIISTFISTRYPELFAKNFDCDVLYVDVGFKNHGNLIANRKKTHNLVVGFEEAIGSLPSDINNDKDSYQTASILLEMINYYYEQKLDLFTVLKKEIFAKYGN", "aa_hexdigest": "5688b731e9af5e5601b05b86fb10c564", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 106, "aa_cov": 0.8412698412698413, "hmm_cov": 0.9528301886792453, "evalue": 2.5481719948050586e-14, "score": 48.98527145385742, "start": 3, "stop": 108 } ], "seq_stats": { "molecular_weight": 14509.431799999982, "isoelectric_point": 6.8981733322143555 }, "id": "GPKLONFDEH_1117", "locus": "GCA000006625_01114" }, { "type": "cds", "sequence": "contig_1", "start": 659466, "stop": 660203, "strand": "-", "frame": 2, "gene": "manB", "product": "Phosphomannomutase", "db_xrefs": [ "COG:COG1109", "COG:G", "SO:0001217", "UniRef:UniRef50_Q9PPW0", "UniRef:UniRef90_Q9PPW0" ], "nt": "TTGACATTAGGTTTTATTGAATATTTAGAAACGAAAAAGAATTTTAATAAAATTATTGTTATTGGCCGAGACAACCGCTTTGGCTCACGTGAAAATTTGAAACTAGTTGCTGAAATTTTTAGTAGTTTTGATTATACTGTTTATATTAATGAAGATTATGGGATGTTATCAACTCCAATTACTTCATTTTTAATTAATCAATTACAAGCAGGAGCTGGAATTATGATCACCGCTTCTCATAATCCTAAAAATTATAATGGTTTTAAAGTTTATAATGCTAATGGTGCTCAGCCATTAGTTGATGATACTAATCTCATTGAATCTTTAATGCCATCATATACAGAAGCATTGGATTTTAACTTTGAATTTAAACAAGATAATATTAGATTTTTAACAAAAGAACAAATTCAAAGTTATTTTGATGCAGTTAAAGCACAACTAATTAATACTGATCCTAATATTAAAAAACCCTTCAAAATTGTTTTTAGTGGTCATCATGGAACAACAACAAAGGACATGATTCCTTTTTTAGAATCACTTGGTTATGATATGGTTAGCGTTAGCGAACAAAATTTTGAAGATCCTAATTTTAATGATGACCCAAGTTCTAATCCAGAAGAACAATGTTCATTTGATTTAAGTGTTGAATATGCAGACAATACAAACGCAGAAATAATGATTGCATCTGATCCTGATGGAGATCGAATGGCCATTGCTGTACGTCATGAAAATTATTGA", "aa": "MTLGFIEYLETKKNFNKIIVIGRDNRFGSRENLKLVAEIFSSFDYTVYINEDYGMLSTPITSFLINQLQAGAGIMITASHNPKNYNGFKVYNANGAQPLVDDTNLIESLMPSYTEALDFNFEFKQDNIRFLTKEQIQSYFDAVKAQLINTDPNIKKPFKIVFSGHHGTTTKDMIPFLESLGYDMVSVSEQNFEDPNFNDDPSSNPEEQCSFDLSVEYADNTNAEIMIASDPDGDRMAIAVRHENY", "aa_hexdigest": "3472ba7b2a3043a907871f09a14fa275", "start_type": "TTG", "rbs_motif": null, "genes": [ "manB" ], "truncated": "both", "pseudogene": { "start": 659163, "stop": 660407, "observations": { "mutation": [ 659468, 660377 ], "stop": [ 659468, 660377 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPW0", "query_cov": 1.0, "subject_cov": 0.4438405797101449, "identity": 0.992, "score": 487.0, "evalue": 2.66e-169, "gene_start": 1, "gene_end": 245, "reference_start": 69, "reference_end": 313, "reference_sequence": "MENTNKIFNCWLNSPKIPQVIKEEYKNKTQKEIDHIFDENYRFKFGTSGVRCIYEEGTRYLNAITYTQLTLGFIEYLETKKNFNKIIVIGRDNRFGSRENLKLVAEIFSSFDYTVYINEDYGMLSTPITSFLINQLQAGAGIMITASHNPKNYNGFKVYNANGAQPLVDDTNLIESLMPSYIEALDFNFEFKQDNIRFLTKEQIQSYFDAVKAQLINTDPNIKKPFKIVFSGHHGTTTKDMIPFLESLGYDMVSVSEQNFEDPNFNDDPSSNPEEQCSFDLSVEYADNTNAEIMIASDPDGDRMAIAVRHENYWKFLTGNQTGVLIAHYLLEHKKFTKPTYIISTFISTRYPELFAKNFDCDVLYVDVGFKNHGNLIANRKKTHDLVVGFEEAIGSLPSDINNDKDSYQTASILLEMINYYYEQKLDLFTVLKKEIFAKYGNWYSKTTQFVIDGDNWKQKALIILNNLRNFEFKTVTQYTIHNIIFHEQGSYLEWKLDEYSTIKFRLSGTEPKFKVYIDLYDFSKDQKRDFYQELKIKAEDILDFLKNYLGL" }, "paralog": false, "identity": 0.9903381642512077, "score": 830.0, "evalue": 4.81e-307, "description": "Internal stop codon at 659468, 660377. Nonsense mutation around 659468, 660377.", "psc": { "uniref90_id": "UniRef90_Q9PPW0", "gene": "ManB", "product": "Phosphomannomutase", "uniref50_id": "UniRef50_Q9PPW0", "cog_id": "COG1109", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPW0", "UniRef:UniRef50_Q9PPW0", "COG:COG1109", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPW0" ], "product": "Phosphomannomutase" } }, "id": "GPKLONFDEH_1118", "locus": "GCA000006625_01115" }, { "type": "cds", "sequence": "contig_1", "start": 660463, "stop": 660837, "strand": "-", "frame": 1, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0005737", "GO:0008270", "GO:0008655", "GO:0009972", "KEGG:K01489", "SO:0001217", "UniRef:UniRef50_Q9PPV9", "UniRef:UniRef90_Q9PPV9" ], "nt": "ATGAAATATAATGATATTTATATGGGATTATTAGAGTTATTAAAAAAAAGTTATAGTCCTTATTCAAATTATCCAGTTGCTGCTTATGTTGATACTGATTTAGGATTTATCTCTGGTGTGAATGTTGAAAATGGATCATTTGGATTAACTTCTTGTGCTGAAAGAAATGCAATCTTTAATGCAATTGCAAATGGTGCAAAAACATTTAAAACAGTTTATATAATTACAAAAAATAATGGTGATATTGCGAGTCCTTGTGGTGCATGTCGTCAAGTGATTTTTGAATTTTTTAAAAAGGATGCAAAAATCGTCGTTTTTAACAATGATGGTACTTATAAAGAATATAATGTTGAACAATTATTACCATTTGGATGA", "aa": "MKYNDIYMGLLELLKKSYSPYSNYPVAAYVDTDLGFISGVNVENGSFGLTSCAERNAIFNAIANGAKTFKTVYIITKNNGDIASPCGACRQVIFEFFKKDAKIVVFNNDGTYKEYNVEQLLPFG", "aa_hexdigest": "720cd4c5b33b51fbfa42b29aec2ee70c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q9PPV9", "query_cov": 1.0, "subject_cov": 0.9465648854961832, "identity": 1.0, "score": 253.0, "evalue": 3.65e-85, "valid": true, "gene": "cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_Q9PPV9", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0005737", "GO:0008270", "GO:0008655", "GO:0009972" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPV9" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "GPKLONFDEH_1119", "locus": "GCA000006625_01116" }, { "type": "tRNA", "sequence": "contig_1", "start": 660962, "stop": 661037, "strand": "-", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 77.3, "nt": "AAGGGTATAGTTCAATTGGTAGAACAGCAGACTCCAAATCTGCGTGTTGCGGGTTCGAGTCCTGTTACCCTTGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 661002, 661004 ], "id": "GPKLONFDEH_12", "locus": "GCA000006625_01117" }, { "type": "cds", "sequence": "contig_1", "start": 661048, "stop": 661590, "strand": "-", "frame": 1, "gene": "pepP", "product": "XAA-PRO aminopeptidase", "db_xrefs": [ "COG:COG0006", "COG:E", "SO:0001217", "UniRef:UniRef50_Q9PPV8", "UniRef:UniRef90_Q9PPV8" ], "nt": "ATGTTAGAATTAGGTGGAGAATTAAATTCATTTGATCCAATTATTGCTTCAGGTCCAAATGGCGGATCACCACATCATCATCCAGGAAATCGTGTAATTGAAGATGGAGATATGGTAACTGTTGACATTGGTTGTACTTATAAAGGTTATTGTTCTGATATTACAAGATCTTTTATTGTTGGAAATAAAGCAAATCCACAAATGCAAGAAATTTATGATAAAGTTCTTGAATCTCAAACAGCAGGGATTAATCTATTGAGTACAAAAGTAACAGGTCAAGAAGTTGATAAAGTATGTCGCGACATTGTTGATAATTCTAAATTTAAGGGCTATTTCACACATGGAACAGGTCATGGTGTAGGTTTACAAGTACATGAATTACCAAATACTAATGCTGGTAATCCTAATAAATTGCCATTAAATGCAGTTGTTACTGTTGAACCAGGAATTTATATCCCAAATGTTGGTGGTGTAAGAATTGAAGATACAATTGTAGTAAAAGACGGACAAGCCCTGGTTTTAACAAGGTTAGCATATAAATAA", "aa": "MLELGGELNSFDPIIASGPNGGSPHHHPGNRVIEDGDMVTVDIGCTYKGYCSDITRSFIVGNKANPQMQEIYDKVLESQTAGINLLSTKVTGQEVDKVCRDIVDNSKFKGYFTHGTGHGVGLQVHELPNTNAGNPNKLPLNAVVTVEPGIYIPNVGGVRIEDTIVVKDGQALVLTRLAYK", "aa_hexdigest": "5fce2ecf2ba68143ace63825cc0f6e8a", "start_type": "ATG", "rbs_motif": null, "genes": [ "pepP" ], "truncated": "3-prime", "pseudogene": { "start": 661048, "stop": 661890, "observations": { "mutation": [ 661656 ], "stop": [ 661656 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPV8", "query_cov": 1.0, "subject_cov": 0.5042016806722689, "identity": 0.961, "score": 362.0, "evalue": 4.52e-124, "gene_start": 1, "gene_end": 180, "reference_start": 178, "reference_end": 357, "reference_sequence": "MSDFKLQQVLKTIKEHEAQGVDGMILFCPYNRYWFLEFASSDGFVFINKDGKAIYLVDARYYTAASEAVKNAKVILLARTPQKSTFDLLKDAMVELNITNALVEADYVTLNVHEMLQKLVRKTTLFTSAALRAIKTEKELEYLQKAADIAALTCNWIREQDIIGRTELEVAMLVSKHMLELGGELNSFDPIIASGPNGGSPHHHPGNRVIEDGDMVTVDIGCTYKGYCSDITRSFIVGNKANPQMQEIYDKVLESQTAGIDLVSTKVTGQEVDKLCRDIIDNSKFNGYFTHGTGHGVGLEVHELPNTNAGNPNKLPLNAVVTVEPGIYIPNVGGVRIEDTVVVKDGQALVLTRLAYK" }, "paralog": false, "identity": 0.9428571428571428, "score": 533.0, "evalue": 6.59e-194, "description": "Internal stop codon at 661656. Nonsense mutation around 661656.", "psc": { "uniref90_id": "UniRef90_Q9PPV8", "gene": "PepP", "product": "XAA-PRO aminopeptidase", "uniref50_id": "UniRef50_Q9PPV8", "cog_id": "COG0006", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPV8", "UniRef:UniRef50_Q9PPV8", "COG:COG0006", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPV8" ], "product": "XAA-PRO aminopeptidase" } }, "id": "GPKLONFDEH_1120", "locus": "GCA000006625_01118" }, { "type": "cds", "sequence": "contig_1", "start": 661654, "stop": 661989, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01321.24" ], "nt": "GTGTTTATTAATAAAGATGGTAAAGCAATTTATCTAGTGGATGCTCGTTATTATACAGCAGCGTCTGAAACTGTAAAAAACGCAAAGGTAATTTTATTAGCTCGCACACCACAAAAATCAACTTTTGATCTACTAAAAGACGCAATGGTTGAATTAAATATTACTAATGCATTAGTTGAAGCTGATTATGTAACATTAGATGTTCATGAAATGTTAAAAAAATTAGTAAAAAATACTACTCCTTTTACATCAGCTACATTACGTGCTATTAAAACTGAAAAAGAACTTGAATATTTACAAAAAGCTGCTGATATTGCAGCTTTAACATGTAATTGA", "aa": "MFINKDGKAIYLVDARYYTAASETVKNAKVILLARTPQKSTFDLLKDAMVELNITNALVEADYVTLDVHEMLKKLVKNTTPFTSATLRAIKTEKELEYLQKAADIAALTCN", "aa_hexdigest": "64b01174e81aba97491a3df719071d6c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01321.24", "name": "Creatinase_N", "length": 90, "aa_cov": 0.7657657657657657, "hmm_cov": 0.9444444444444444, "evalue": 3.5507761502425984e-11, "score": 39.33312225341797, "start": 2, "stop": 86 } ], "seq_stats": { "molecular_weight": 12414.310299999983, "isoelectric_point": 7.785476112365723 }, "id": "GPKLONFDEH_1121", "locus": "GCA000006625_01119" }, { "type": "cds", "sequence": "contig_1", "start": 662020, "stop": 662121, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGATTTCAAATTACAACAAGTTTTAAAAACAATTAAAGAACATGAAGCACAAGGCGCTGACGGTATGATTTTATTTTCTAATTATAACCGTTATTGA", "aa": "MSDFKLQQVLKTIKEHEAQGADGMILFSNYNRY", "aa_hexdigest": "184ab835660db9db48511aae840475b6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3876.3747000000003, "isoelectric_point": 6.517920494079591 }, "id": "GPKLONFDEH_1122", "locus": "GCA000006625_01120" }, { "type": "cds", "sequence": "contig_1", "start": 662132, "stop": 662293, "strand": "-", "frame": 3, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "COG:COG0267", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_006688661.1", "SO:0001217", "UniParc:UPI0000133F9B", "UniRef:UniRef100_Q9PPV7", "UniRef:UniRef50_P47567", "UniRef:UniRef90_Q9PPV7" ], "nt": "ATGGCGATTAAACGAGGCGTTAGATTACAATGTAATGAGAGTAAAAGTATTAATTACATTACTACAAAAAATGCAAAAAATAATCCAGACAGACTTTCTTTAAATAAATTTTGTCCTAAATGTCGAAAAGTAACAACTCACGTTGAAATTAAGAAAAAATAG", "aa": "MAIKRGVRLQCNESKSINYITTKNAKNNPDRLSLNKFCPKCRKVTTHVEIKKK", "aa_hexdigest": "4e79c0d409751519124c9bf14d44291d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0000133F9B", "ncbi_nrp_id": "WP_006688661.1", "uniref100_id": "UniRef100_Q9PPV7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133F9B", "RefSeq:WP_006688661.1", "UniRef:UniRef100_Q9PPV7" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPV7", "uniref90_id": "UniRef90_Q9PPV7" }, "psc": { "uniref90_id": "UniRef90_Q9PPV7", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P47567", "cog_id": "COG0267", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47567", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47567" ], "product": "Large ribosomal subunit protein bL33A" }, "genes": [ "rpmG" ], "id": "GPKLONFDEH_1123", "locus": "GCA000006625_01121" }, { "type": "cds", "sequence": "contig_1", "start": 662663, "stop": 662941, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGTTAAAAGATCACAATATTCCTTATAAGGAGATGGTAGGAGAAGCGGCATTTTATGGTCCAAAAATCGATTTTCAGGTTAAAACTGTTTTAAATCGAATTATTACAGTTTCAACTATCCAATTAGATTTTTTATTACCAAACCGTTTTAATTTAACTTATATTAATGAAAGTAATGAACAAAGCGTACCGGTAATGATTCATATTGGTATTATTGGAACTTATGAACGTTTATTAGCAATTTTATTAGAACAAACTAAAGGTATTTTACCGCTTTGA", "aa": "MLKDHNIPYKEMVGEAAFYGPKIDFQVKTVLNRIITVSTIQLDFLLPNRFNLTYINESNEQSVPVMIHIGIIGTYERLLAILLEQTKGILPL", "aa_hexdigest": "a0975fb11fd9de7f1a799046f5c8619f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0029CA135A", "query_cov": 0.9021739130434783, "subject_cov": 0.8217821782178217, "identity": 0.614, "score": 100.0, "evalue": 6.18e-26, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_Q71HR4", "kegg_orthology_id": "K01868" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 73, "aa_cov": 0.7717391304347826, "hmm_cov": 0.9726027397260274, "evalue": 4.056435221966332e-16, "score": 55.148681640625, "start": 15, "stop": 85 } ], "seq_stats": { "molecular_weight": 10516.25699999999, "isoelectric_point": 6.048600959777832 }, "id": "GPKLONFDEH_1124", "locus": "GCA000006625_01122" }, { "type": "cds", "sequence": "contig_1", "start": 662969, "stop": 663289, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGACATGTCCTCATCATACATTAGTTTACTCTAATGAACTTCGTTCATATCGAAGTTTACCAATTCGTTTAAGTGAACATTCAATTTTACATCGTTATGAATCATCAGGTGGATTAACAGGCTTTGAGCGGGTTCGTGAGATGATTTTAGAAGATTGTCATGTTTTTTGTCGCTTTGATCAAATTGAACATGAAGTTATTAATGCTTTTAAAATGATTCAAGAAGCTCAAGAAGGATTGGGTATTAAAACATTTGAAATTCATTTATCTTTAAATGATCCTAATGATAAAGAAAAATATTATGATGATCCTCAAATGTGA", "aa": "MTCPHHTLVYSNELRSYRSLPIRLSEHSILHRYESSGGLTGFERVREMILEDCHVFCRFDQIEHEVINAFKMIQEAQEGLGIKTFEIHLSLNDPNDKEKYYDDPQM", "aa_hexdigest": "3de92c66b3676cb96fccdd6e10ef6a83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 94, "aa_cov": 0.8773584905660378, "hmm_cov": 0.9680851063829787, "evalue": 9.593972291104868e-19, "score": 63.704036712646484, "start": 1, "stop": 93 } ], "seq_stats": { "molecular_weight": 12529.014699999989, "isoelectric_point": 5.399613380432128 }, "id": "GPKLONFDEH_1125", "locus": "GCA000006625_01123" }, { "type": "cds", "sequence": "contig_1", "start": 663671, "stop": 664096, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "ATGTATAAGTTTGATCAAAAACTAAATCACAGTGCTGCACATCTTTTAGCAATGGCACTAACAAAATTTTATCCTAACTTAAGCCTTGCGATTGGGCCAACGATTGATGAGGGGTTTTATTATGATTTTAATCTAAATGATCCAAATACCTCAATTACCCCTTTAGATTTATTAAAAATTGAAAAAGAAATGAAGAAAATTACTACTCAAGCTTTAACGTTTGATTATGAACAAGTTACTTATGAAAAAGCAAAGGAATTGTTTAAACACAATAAATATAAATTAGATATTATTGAACAAAATAAAAATAATTCACTTTCAATTTATCACTCAGGTAAATGGTTTGACTTATGTAAAGGCCCGCATGTACAAAACACAAAAGAGATCAAGGCAATTAAATTATTAAATATTGCAGGTTCATATTGA", "aa": "MYKFDQKLNHSAAHLLAMALTKFYPNLSLAIGPTIDEGFYYDFNLNDPNTSITPLDLLKIEKEMKKITTQALTFDYEQVTYEKAKELFKHNKYKLDIIEQNKNNSLSIYHSGKWFDLCKGPHVQNTKEIKAIKLLNIAGSY", "aa_hexdigest": "e4ee9bfdcd1cc245a42009444f54881b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 31, "aa_cov": 0.2127659574468085, "hmm_cov": 1.0, "evalue": 7.12924004843848e-13, "score": 43.974327087402344, "start": 107, "stop": 136 } ], "seq_stats": { "molecular_weight": 16338.599799999993, "isoelectric_point": 8.491018104553223 }, "id": "GPKLONFDEH_1126", "locus": "GCA000006625_01124" }, { "type": "cds", "sequence": "contig_1", "start": 664324, "stop": 664833, "strand": "+", "frame": 1, "gene": "degV", "product": "DegV domain-containing protein UU535", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "SO:0001217", "UniRef:UniRef50_Q9PPV5", "UniRef:UniRef90_Q9PPV5" ], "nt": "ATGATTCTGCCGCTTTCTATTTTACGAAGTGATCATACTTTAATTGTAGATGATGGTATAGAATCAAAGCCAGAGCGAATTTATGAAGACATTGATAATGGATACACCTTTCAAACAAGTTGTACACCATATGGTGTTTTAATTGAAGCTATTGAACAAAAACTACAAGAATATGAAAAAATTATTTTTATTGGCATCTCTAGTGGATTTAGTAGTCAATTCAATAATGCAAAAAATTTAGAGAAAGAATATCAAGACAAATTATTTGTTGTAGACACAGAAGACTTTGGCTATTCCTTAGAACATCTAGTTTACAAAATTAAAGCAATGTTAAGCAATAATATTAGTTTTGGTGATATTTTAAAAATGATTAACAAACACCATGATTATACAAGTAGTTTTTTAGCATGTGAAAATATTACTGGCCTTGTTCGTTCTGGTCGAATCCCTAAAATCATTGGTACTATGTTAAAATTAAGTAAAGTAACACCCATAATTAAAGCTGAATGA", "aa": "MILPLSILRSDHTLIVDDGIESKPERIYEDIDNGYTFQTSCTPYGVLIEAIEQKLQEYEKIIFIGISSGFSSQFNNAKNLEKEYQDKLFVVDTEDFGYSLEHLVYKIKAMLSNNISFGDILKMINKHHDYTSSFLACENITGLVRSGRIPKIIGTMLKLSKVTPIIKAE", "aa_hexdigest": "19c0d65a05ce72f42be7037cb889438d", "start_type": "ATG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "both", "pseudogene": { "start": 664249, "stop": 665136, "observations": { "mutation": [ 664303, 664831, 665077 ], "stop": [ 664303, 664831, 665077 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPV5", "query_cov": 1.0, "subject_cov": 0.5399361022364217, "identity": 0.976, "score": 332.0, "evalue": 4.74e-113, "gene_start": 1, "gene_end": 169, "reference_start": 26, "reference_end": 194, "reference_sequence": "MKKSFLIMTDSSTTLDREWAKNNDVMILPLSILRSDHTLIVDDGIESKPERIYEDIDNGYTFQTSCTPYGALIEAIEQKLQEYEKIIFIGISSGFSSQFSNAKNLEKEYQDKLFVVDTEDFGYSLEHLVYKIKVMLTNNISFGDILKMINKHHDYTSSFLACENITGLVRSGRIPKIIGTMLKLSKVTPIIKAEWKNHRAGMALNIRSAPHKILENISHVFDNQLNNHTIEKVCILQAGLSNERIDELKNDVINHFHIDKEKIVIRSGPPIFLVYVWKGALGIQVIANIPKKHVEKNINNKLYTDIIHKQGLI" }, "paralog": false, "identity": 0.9661016949152542, "score": 565.0, "evalue": 2.82e-207, "description": "Internal stop codon at 664303, 664831, 665077. Nonsense mutation around 664303, 664831, 665077.", "psc": { "uniref90_id": "UniRef90_Q9PPV5", "gene": "DegV", "product": "DegV domain-containing protein UU535", "uniref50_id": "UniRef50_Q9PPV5", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPV5", "UniRef:UniRef50_Q9PPV5", "COG:COG1307", "COG:I", "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPV5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPV5" ], "product": "DegV domain-containing protein UU535" } }, "id": "GPKLONFDEH_1127", "locus": "GCA000006625_01125" }, { "type": "cds", "sequence": "contig_1", "start": 664852, "stop": 665079, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTAAATATTCGTTCTGCACCCCATAAAATTCTTGAAAATATTAACCATGTTTTTGATAATCAACTAAATAATCATACAATTGAAAAAGTATGTATTCTACAAGCGGGATTATCAAGTGAAAGAATTGATGAACTAAAAAATGATGTAATTAACCATTTTCATGTTGATAAGGAAAAGATTGTAATTAGATCTGGTCCTCCAATCTTTTTAGTATATGTTTGA", "aa": "MALNIRSAPHKILENINHVFDNQLNNHTIEKVCILQAGLSSERIDELKNDVINHFHVDKEKIVIRSGPPIFLVYV", "aa_hexdigest": "332312d60edbd35b9a851b5f47dbc10e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8612.853899999996, "isoelectric_point": 6.490069389343262 }, "id": "GPKLONFDEH_1128", "locus": "GCA000006625_01126" }, { "type": "cds", "sequence": "contig_1", "start": 665142, "stop": 665294, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01416.25" ], "nt": "ATGGTTCGTATGATCGTTGCTTCTTGTTTAGATTATGCTTTTAATAAAATAAGTTTAGCAAAAATTCATGAATTATTAATATACCCCAAAAAGGGTGCTAGTATTAAATTAGCACCCGCTTGTGGATTATATTTGTATAAAGTCTATTATTAA", "aa": "MVRMIVASCLDYAFNKISLAKIHELLIYPKKGASIKLAPACGLYLYKVYY", "aa_hexdigest": "72362493aa539bdf4955dbc25fa60c4e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01416.25", "name": "PseudoU_synth_1", "length": 51, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 9.573591710951364e-08, "score": 28.113216400146484, "start": 1, "stop": 50 } ], "seq_stats": { "molecular_weight": 5684.885400000001, "isoelectric_point": 9.429677772521973 }, "id": "GPKLONFDEH_1129", "locus": "GCA000006625_01127" }, { "type": "cds", "sequence": "contig_1", "start": 665358, "stop": 665591, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAAGGTTGATGATTCATTTCATGCTCAATATAATGCTACTTCTAAAACATATCAATATGTCATTAATGATTATGAATTTGATTTGTTTAGAAATAATTATGAACTTTTTGTTAATCAAAAAATTAATGATCAAAAAATATTAGAAGCATTAGAATTGTTTGTTGGCGAACATGATTTTAAATCGTTTTCAACTAGTGAATTAACAATGACAATACGTCGCATTAATTGA", "aa": "MQKVDDSFHAQYNATSKTYQYVINDYEFDLFRNNYELFVNQKINDQKILEALELFVGEHDFKSFSTSELTMTIRRIN", "aa_hexdigest": "5512a02d0d1258140d2635cae00c63f5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9267.208299999993, "isoelectric_point": 4.943366813659667 }, "id": "GPKLONFDEH_1130", "locus": "GCA000006625_01128" }, { "type": "cds", "sequence": "contig_1", "start": 665929, "stop": 666549, "strand": "-", "frame": 1, "gene": "ecfT", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "db_xrefs": [ "COG:COG0619", "COG:H", "KEGG:K16785", "SO:0001217", "UniRef:UniRef50_A0A0C5RLA6", "UniRef:UniRef90_A0AAC9T2R8" ], "nt": "TTGTCAGGTGTAGGTGATCAATTAGGTAAGGCTCAAACCGCTTTATTTTATACACGAAGTCCTTTCGCTTTAAGTCCTATTGCTATTCAATTAGCTGTTTATATATCAATAAAAATTTTTTTAATGATAACCTTATCATCAATTCTAACAGCTACGACTTCATCTATTGAATTGACAAATGGTTTGGAAGATTTGTTAAGTCCTTTTAAAATTTTACGATTGCCTGTTGCTGAGGCTTCAATGATGATTTCAATTGCATTACGTTTTATTCCGTCATTATTAGATGAATCAAAAAGAATATTAAATGCACAAGCTTCACGTGGTGTTGATTTTAATAATGGCGGACCTATTCAAAAATTAAAATCATTAATTTCACTAGTTGTACCATTATTTTCAATTGCTTTTAAAAAAGCAGAAGATTTAGCAAATGCTATGGAAGCACGTTCTTATAACCCTCGTTATGCACGAACACGTTATCGTACTTTTCCATTAAACCTAGCAGATTATGCTTTATTTGGAATTTTGTGTATTCTCGTTGGATTTTTAATATCATTGGCCGTGATTAAATTTTATTTCACTCCATTTGGTGTATTTGAAGCAAGTGCATTGTTTGCTAAGTAA", "aa": "MSGVGDQLGKAQTALFYTRSPFALSPIAIQLAVYISIKIFLMITLSSILTATTSSIELTNGLEDLLSPFKILRLPVAEASMMISIALRFIPSLLDESKRILNAQASRGVDFNNGGPIQKLKSLISLVVPLFSIAFKKAEDLANAMEARSYNPRYARTRYRTFPLNLADYALFGILCILVGFLISLAVIKFYFTPFGVFEASALFAK", "aa_hexdigest": "d6e19310319db4f219e8adae4d14aa4f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ecfT" ], "truncated": "3-prime", "pseudogene": { "start": 665929, "stop": 666849, "observations": { "mutation": [ 666585, 666837 ], "stop": [ 666585, 666837 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T2R8", "query_cov": 1.0, "subject_cov": 0.4703196347031963, "identity": 0.951, "score": 365.0, "evalue": 8.04e-124, "gene_start": 1, "gene_end": 206, "reference_start": 233, "reference_end": 438, "reference_sequence": "MSANAYVFRRSPIHRLNPAIKFISFILLIAMIFLPLGFFAQMIIGVFILIIFFVAKLPKKTLWNVFKSVIMLFVILLLINWMTYKDPIAIYNITDQAKVILGDKDWINGPINKNLSFSLIYNDISSTHVQNLVSNIWGGEIKNYISPEIIKKLIDKPDYNVAKFLSENNITVKKLASTFNALNQDVRLNNYYPIYGDAVLRSGKVEVPLSHLSYYMSTNLWKIEGVKYQGLILSGVGDQLGKAETALFYTRSPFALSPVAIQLAIYISIKIFLMITLSSILTATTSSIELTNGLEDLLSPFKILRLPVAEASMMISIALRFIPSLLDESKRILNAQASRGVDFNNGGMLQKLKSLISLVVPLFSIAFKKAEDLANAMEARSYNPRYARTRYRAFPLNLTDYVLFGILCILVGFLISLAVIKFYFTPFGAFEASALFAK" }, "paralog": false, "identity": 0.9281045751633987, "score": 553.0, "evalue": 4.09e-200, "description": "Internal stop codon at 666585, 666837. Nonsense mutation around 666585, 666837.", "psc": { "uniref90_id": "UniRef90_A0AAC9T2R8", "gene": "EcfT", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "uniref50_id": "UniRef50_A0A0C5RLA6", "cog_id": "COG0619", "cog_category": "H", "kegg_orthology_id": "K16785", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T2R8", "UniRef:UniRef50_A0A0C5RLA6", "COG:COG0619", "COG:H", "KEGG:K16785" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0C5RLA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C5RLA6" ], "product": "Cobalt transporter" } }, "id": "GPKLONFDEH_1131", "locus": "GCA000006625_01129" }, { "type": "cds", "sequence": "contig_1", "start": 667057, "stop": 667245, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGCTAATGCTTATGTGTTTCGACGTTCACCAATTCATCGTTTAAATCCAGCAATTAAATTTATTTCTTTTATTTTATTAATTGCTATGATTTTTTTACCACTTGGCTTTTTTGCGCAAATGATTATTGGAATTTTTATTTTAATCGTCTTTTTTGTAGCTAAATTACCTAAGCGTACTTTATGA", "aa": "MSANAYVFRRSPIHRLNPAIKFISFILLIAMIFLPLGFFAQMIIGIFILIVFFVAKLPKRTL", "aa_hexdigest": "9960f0a70270e55dd54b2cd764d647ce", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7140.828099999996, "isoelectric_point": 11.999967765808105 }, "id": "GPKLONFDEH_1132", "locus": "GCA000006625_01130" }, { "type": "cds", "sequence": "contig_1", "start": 667246, "stop": 668118, "strand": "-", "frame": 1, "gene": "ecfA2", "product": "Energy-coupling factor transporter ATP-binding protein EcfA2", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:3.6.3.-", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_Q9PPV2", "UniRef:UniRef90_Q9PPV2" ], "nt": "TTGTTTGAAGCAAATTTTAAACAAAAACCAATTAGTTTAAAATTTATTAAAACAAAAAATAATCATGAATTAATTACAAATCCTTATGTAAGAGAAAACACTAAAATAGCAGTAGTTCGTGTTGATAAAAATGTTATATTAGAAATTAATGATAAGATGAATTATGAAGAGTTACAACGTTTTGAATTTATTAAAAAAGAAATTAAGACGAACTATCATTTATCAAAAAAAATAAAGCGGTTTAAAGAATTACGTAGACGTGTTGGTTTTGTATTCCAATTCCCTGAATACCAATTGTTTAAGGATACTATTGAAAAAGACATCATGTTTGGTCCAGTTAATTTAGGTGTAAAAAAAAGCGAAGCTAAAAAACGAGCTAAATTTTATTTAAATAAATTAGGTTTAGGAGATGATTATTTAGAACGCTCACCTTTTGGATTATCTGGAGGCCAAAAACGCCGAGTAGCAATTGCTGGAATTTTAGCAATCCAAAATGATATTTTAGTTTTTGATGAACCAACAGCTGGTTTAGATCCTGCAGGTGAACACGAAATGATGCAAATTATTTTAGATGCGAAAGCAAATAATAAAACAGTTTTTGTTATTACCCATACTATGGAACATGTGCTTGAAGTTGCTGATGAAGTGATTGTAATGGATGAAGGTGAAATTATTAAAACAGGGACACCTTATGAAATTTTTTTTGATCAACACATTATTAATTCAACTTCAATTCAAGTTCCTCGTGTAATTGCAGTTATTAATGAATTGATTAAAAAAGATCTTAAATATGAAATTCTAAAACAAAAACAACCACGCACAATTGAAGAGTTAGCAGATGCTATCATCGAATTTAAAAAAGGAGAAAAATAA", "aa": "MFEANFKQKPISLKFIKTKNNHELITNPYVRENTKIAVVRVDKNVILEINDKMNYEELQRFEFIKKEIKTNYHLSKKIKRFKELRRRVGFVFQFPEYQLFKDTIEKDIMFGPVNLGVKKSEAKKRAKFYLNKLGLGDDYLERSPFGLSGGQKRRVAIAGILAIQNDILVFDEPTAGLDPAGEHEMMQIILDAKANNKTVFVITHTMEHVLEVADEVIVMDEGEIIKTGTPYEIFFDQHIINSTSIQVPRVIAVINELIKKDLKYEILKQKQPRTIEELADAIIEFKKGEK", "aa_hexdigest": "86ed6f3315642d7049f624b5b17e3eef", "start_type": "TTG", "rbs_motif": null, "genes": [ "ecfA2" ], "truncated": "3-prime", "pseudogene": { "start": 667246, "stop": 668418, "observations": { "mutation": [ 668193 ], "stop": [ 668193 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPV2", "query_cov": 1.0, "subject_cov": 0.6697459584295612, "identity": 0.997, "score": 555.0, "evalue": 4.82e-197, "gene_start": 1, "gene_end": 290, "reference_start": 144, "reference_end": 433, "reference_sequence": "MQKLMDYFFKRKLKVPRPISNDISVRVKNLYAVYDEKQENQLVALNNISYDFKKNKIYFIIGNSGSGKSTLVTHFNGLMISRYGFVQVGDIVSGDHFDFERQLLGVIDSYDKKIINLLWRNQLDQWTFLVLFSNEVNIQQARILFEANFKQKPISLKFIKTKNNHELITNPYVRENTKIAVVRVDKNVILEINDKMNYEELQRFEFIKKEIKTNYHLSKKIKRFKELRRRVGFVFQFPEYQLFKDTIEKDIMFGPVNLGVKKSEAKKRAKFYLNKLGLGDDYLERSPFGLSGGQKRRVAIAGILAIQNDILVFDEPTAGLDPAGEHEMMQIILDAKANNKTVFVITHTMEHVLEVADEVIVMDEGEIIKTGTPYEIFFDQHIINSTSIQVPRVIAVINELIKKDLKYEILKQKQPRTIEELADAIIEFKKGEK" }, "paralog": false, "identity": 0.9974358974358974, "score": 765.0, "evalue": 1.83e-282, "description": "Internal stop codon at 668193. Nonsense mutation around 668193.", "psc": { "uniref90_id": "UniRef90_Q9PPV2", "gene": "ecfA2", "product": "Energy-coupling factor transporter ATP-binding protein EcfA2", "ec_ids": [ "3.6.3.-" ], "uniref50_id": "UniRef50_Q9PPV2", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPV2", "EC:3.6.3.-", "UniRef:UniRef50_Q9PPV2", "COG:COG1122", "COG:PR", "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPV2" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" } }, "id": "GPKLONFDEH_1133", "locus": "GCA000006625_01131" }, { "type": "cds", "sequence": "contig_1", "start": 668191, "stop": 668547, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCAAAAATTAATGGATTATTTTTTCAAAAGAAAACTTAAAGTACCACGACCAATATCTAATGATATTAGTGTTCGTGTAAAAAATTTATATGCTGTTTATGATGAAAAACAAGAAAATCAATTAGTCGCATTAAATAATATTTCGTATGATTTTAAAAAAAATAAAATCTATTTTATTATTGGAAATTCTGGTTCTGGTAAATCTACGCTAGTTACTCATTTTAATGGATTAATGATCTCGCGTTATGGTTTCGTTCAAGTCGGTGATATTGTTTCTGGTGATCATTTTGATTTTGAACGCCAATTATTAGGAGTGATTGATTCATATGATAAAAAAATTATTAATTTATTATGA", "aa": "MQKLMDYFFKRKLKVPRPISNDISVRVKNLYAVYDEKQENQLVALNNISYDFKKNKIYFIIGNSGSGKSTLVTHFNGLMISRYGFVQVGDIVSGDHFDFERQLLGVIDSYDKKIINLL", "aa_hexdigest": "f4bd74347905888e95181584bc97e125", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 46, "aa_cov": 0.3898305084745763, "hmm_cov": 1.0, "evalue": 7.652669401823253e-10, "score": 35.1241569519043, "start": 45, "stop": 90 } ], "seq_stats": { "molecular_weight": 13674.65609999999, "isoelectric_point": 9.424778175354007 }, "id": "GPKLONFDEH_1134", "locus": "GCA000006625_01132" }, { "type": "cds", "sequence": "contig_1", "start": 668535, "stop": 668879, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATTTTTGATGAATCCACAAGTATGTTAGATCCCAAAGGAAAAAATGAATTAAAAGAATTAATGGTTTCATTGCGTGATGTTGCAAAAAAAACAATTATTTCTATTACTCATGATATGGAAGAAGTAGTTAAAGCTGATAAAGTTATTGTCATGTCAAATGGCGAAGTTCAATATATAGGTACACCACAAGAAATTTTTGCTAATGAAGAACGTTTATTAAAAATGCAATTAGATATTCCATTTACATTAAAATTAGCAAAAACCTTAAAGGAAAAGGGGTTAAAAATTGATTTAACTTTGAATAATGAGGAGTTAATTGAAAAAATATGCAAAAATTAA", "aa": "MIIFDESTSMLDPKGKNELKELMVSLRDVAKKTIISITHDMEEVVKADKVIVMSNGEVQYIGTPQEIFANEERLLKMQLDIPFTLKLAKTLKEKGLKIDLTLNNEELIEKICKN", "aa_hexdigest": "59522340d2745721e0c642bf2be8dea8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13021.211799999983, "isoelectric_point": 5.258027458190917 }, "id": "GPKLONFDEH_1135", "locus": "GCA000006625_01133" }, { "type": "cds", "sequence": "contig_1", "start": 669006, "stop": 669419, "strand": "-", "frame": 2, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "EC:7.-.-.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47425", "UniRef:UniRef90_Q9PPV1" ], "nt": "ATGAGTCATAATGATAAAAATACTCATAATTATCAAATTTCATCATTAGATAAGATTATTAATGATTCAAGTGTTGCCATTGAATTTGAAAATGTTTATTTTGCTTATACAGAAGAACGAATGATTTTAAAAAATGTTTCATTTACAATTAATGACAATGAATATGTTTGTGTTATTGGTCATAATGGTTCTGGAAAATCAACGATATCTAAGGTTTTAACAGGATTATTAAAACCTAAAAGCGGTGTAATTAAACTTTTTGGAATTGAGATAAGTGCTGCTAATTTAAAATATTTGCGAAATAATATCGGAATTGTTTTTCAGAATCCTGATAATCAATTTGTAGGTATTACGGCTGAAGATGATATTGCTTTTGGTTTAGAAAATCGTAAGGTTCCGCCAAATAAAATGTGA", "aa": "MSHNDKNTHNYQISSLDKIINDSSVAIEFENVYFAYTEERMILKNVSFTINDNEYVCVIGHNGSGKSTISKVLTGLLKPKSGVIKLFGIEISAANLKYLRNNIGIVFQNPDNQFVGITAEDDIAFGLENRKVPPNKM", "aa_hexdigest": "97849929e4a480558733cd3aa47c7cdc", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ecfA1" ], "truncated": "5-prime", "pseudogene": { "start": 668703, "stop": 669419, "observations": { "mutation": [ 669008 ], "stop": [ 669008 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPV1", "query_cov": 1.0, "subject_cov": 0.46598639455782315, "identity": 1.0, "score": 273.0, "evalue": 1.75e-90, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MSHNDKNTHNYQISSLDKIINDSSVAIEFENVYFAYTEERMILKNVSFTINDNEYVCVIGHNGSGKSTISKVLTGLLKPKSGVIKLFGIEISAANLKYLRNNIGIVFQNPDNQFVGITAEDDIAFGLENRKVPPNKMWDIINDAAVATGIEDLLKKESLELSGGQKQRVAIASVLAINPKVIIFDESTSMLDPKGKNELKELMVSLRDVAKKTIISITHDMEEVVKADKVIVMSNGEVQYIGTPQEIFANEERLLKMQLDIPFTLKLAKTLKEKGLKIDLTLNNEELIEKICKN" }, "paralog": false, "identity": 0.9957983193277311, "score": 458.0, "evalue": 6.02e-166, "description": "Internal stop codon at 669008. Nonsense mutation around 669008.", "psc": { "uniref90_id": "UniRef90_Q9PPV1", "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "7.-.-.-" ], "uniref50_id": "UniRef50_P47425", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPV1", "EC:7.-.-.-", "UniRef:UniRef50_P47425", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_P47425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47425" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" } }, "id": "GPKLONFDEH_1136", "locus": "GCA000006625_01134" }, { "type": "cds", "sequence": "contig_1", "start": 669559, "stop": 670248, "strand": "-", "frame": 1, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "COG:COG0081", "COG:J", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843", "KEGG:K02863", "RefSeq:WP_006688527.1", "SO:0001217", "UniParc:UPI00000C1CAF", "UniRef:UniRef100_Q9PPV0", "UniRef:UniRef50_P47328", "UniRef:UniRef90_Q9PPV0" ], "nt": "ATGGCTAAAATTTCTAAAAAATTATTTGCTGCTTATGAAGGAATTGATAAGCAAAAAGCTTACCCATTATTTGATGCAATTAAATTAGCACAAGAGAAGTCAATTACTAAATTTGATGGATCAATTAATATCGCTATTAAATTGAATTTAGATACTACTAAAGTTGAACAACAATTACGTGGGTCAATTTCATTGCCAAACGGTAATGGTAAAAATGTACGAGTTCTTGTTTTAAGTGAAGATATTACCAAAGAAGAGGCTGCTTCAGTTGGTGCTGATTATTTTGGTGGAGCAGATTACATTCAAAATATTGAAAAGATGTTAAACCAAATTGATGTAATTATTACTAACCAAAAAATGATGCCATTATTAGCTAAATTAGGAAAAGTATTGGGGCCTCGTGGACTAATGCCTAATCCAAAAATTGGAACAGTTACAAATGATGTTCTAAAAGCTGTAGAAGAATTTAAAAGAGGTCGAATTGAATACCGTACAGATACTTATGGAAATATCCATATGAGTATTGGTCGTGTTTCATTTGAAACAACTAAAATTGAAGAAAATGCTAATGCATTATTAAACTTAATTAAATCAAAAAAACCAGCGACTGTAAAGGGTCAATATATTCAAAATATTGCTGTTTCACCTACAATGGGTCCTGGAATAAAAGTTGTTATTAATAATAATTAA", "aa": "MAKISKKLFAAYEGIDKQKAYPLFDAIKLAQEKSITKFDGSINIAIKLNLDTTKVEQQLRGSISLPNGNGKNVRVLVLSEDITKEEAASVGADYFGGADYIQNIEKMLNQIDVIITNQKMMPLLAKLGKVLGPRGLMPNPKIGTVTNDVLKAVEEFKRGRIEYRTDTYGNIHMSIGRVSFETTKIEENANALLNLIKSKKPATVKGQYIQNIAVSPTMGPGIKVVINNN", "aa_hexdigest": "f400fd6cccec882aa3214c5ee0845822", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1CAF", "ncbi_nrp_id": "WP_006688527.1", "uniref100_id": "UniRef100_Q9PPV0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CAF", "RefSeq:WP_006688527.1", "UniRef:UniRef100_Q9PPV0" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPV0", "uniref90_id": "UniRef90_Q9PPV0" }, "psc": { "uniref90_id": "UniRef90_Q9PPV0", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_P47328", "cog_id": "COG0081", "cog_category": "J", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P47328", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47328" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "GPKLONFDEH_1137", "locus": "GCA000006625_01135" }, { "type": "cds", "sequence": "contig_1", "start": 670251, "stop": 670703, "strand": "-", "frame": 2, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180", "KEGG:K02867", "SO:0001217", "UniParc:UPI00000C1CB0", "UniRef:UniRef100_Q9PPU9", "UniRef:UniRef50_P47327", "UniRef:UniRef90_Q9PPU9" ], "nt": "GTGGCTCCTAAAAAGAAAGAAGTTACAAGAATCGCAAAGTTAAATCTAATTGGTGGACAAGCTAAACCTGGTCCAGCATTAGCATCAGTTGGTATTAATATGGCTGAGTTTACTAAGTCATTTAATGATAAAACTAAAGACCAAAACGGTAAGGTTATCCCAGTAATTATTACAGCTTATAAAGATAAATCATTTGATTATGTTATCAAAACTACTCCCGTGACTTTTTTACTAAAAGATATTGCTAAAATCAAAAGTGGTGCCAAAGATCCTAAAAAGCAAACTGTTGCAACAATTTCAAAAGAACAAGCTTTAGAAATAGCTCGTTATAAATTGATTGATATGACAGCATATGATGAAGAAGCCGCTTTAAGAATGATTGCTGGTTCTGCAAAACAAATGGGAATTGTAATTGAAGGCGTAAGTGCATATAAAGAAAAGAAAGGTAACTAA", "aa": "MAPKKKEVTRIAKLNLIGGQAKPGPALASVGINMAEFTKSFNDKTKDQNGKVIPVIITAYKDKSFDYVIKTTPVTFLLKDIAKIKSGAKDPKKQTVATISKEQALEIARYKLIDMTAYDEEAALRMIAGSAKQMGIVIEGVSAYKEKKGN", "aa_hexdigest": "d8841321245920a07dc507291e254c7b", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00000C1CB0", "uniref100_id": "UniRef100_Q9PPU9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CB0", "UniRef:UniRef100_Q9PPU9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPU9", "uniref90_id": "UniRef90_Q9PPU9" }, "psc": { "uniref90_id": "UniRef90_Q9PPU9", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_P47327", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P47327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47327" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "GPKLONFDEH_1138", "locus": "GCA000006625_01136" }, { "type": "cds", "sequence": "contig_1", "start": 670982, "stop": 671407, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D2A8F1", "UniRef:UniRef50_UPI0029075D7D", "UniRef:UniRef90_UPI0029075D7D" ], "nt": "ATGAAAAAAATGTTTCTATTAATGAAGAATATGATGCGTTTATTCTTTAAAAATAAAACTTTAGTAACTCAACTTTCAATTTTAATGATTGTTTCAACAGTTGTTATTGTTGCAACAACGATTACAGTACAACGTTTGCAAAAATCACAAAGCGATATTAAAAAAATTGGTTTACAAAGTGATTTTTTAATTGATACAAAAGAGCAAGATAATATTAATTTCAACGAAATTGACGTTAGCAACAATCTTGTAAAACATAATGAAAAATTAATTCAATTAGCAACTTTTAATCAAAATATTTTAAAAAATTCAAATAATTATTTACAACTAGTTGATAATGCATTTCATAATCATAACCTTTTTTGGCCAACTAATGTAAACAATCAAAAAATAGATGTACTAAATCTTGAAGGAAATATCGATTGA", "aa": "MKKMFLLMKNMMRLFFKNKTLVTQLSILMIVSTVVIVATTITVQRLQKSQSDIKKIGLQSDFLIDTKEQDNINFNEIDVSNNLVKHNEKLIQLATFNQNILKNSNNYLQLVDNAFHNHNLFWPTNVNNQKIDVLNLEGNID", "aa_hexdigest": "1c16f4d5b28f53c2875b6cf593ed75b6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0002D2A8F1", "query_cov": 1.0, "subject_cov": 0.844311377245509, "identity": 0.872, "score": 228.0, "evalue": 1.97e-74, "valid": false, "uniref50_id": "UniRef50_UPI0002D2A8F1" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D2A8F1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D2A8F1" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 670982, "stop": 671626, "observations": { "mutation": [ 671405, 671444 ], "stop": [ 671405, 671444 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029075D7D", "query_cov": 1.0, "subject_cov": 0.618421052631579, "identity": 1.0, "score": 257.0, "evalue": 2.85e-85, "gene_start": 1, "gene_end": 141, "reference_start": 1, "reference_end": 141, "reference_sequence": "MKKMFLLMKNMMRLFFKNKTLVTQLSILMIVSTVVIVATTITVQRLQKSQSDIKKIGLQSDFLIDTKEQDNINFNEIDVSNNLVKHNEKLIQLATFNQNILKNSNNYLQLVDNAFHNHNLFWPTNVNNQKIDVLNLEGNIDWNKINQLKLQTVFWFENNKENNQNFANNYDDLVYEYHNSKNQLVGFQNLDGGHILFKTPALKSANGNYETTFKFWNPTNWFLELWYS" }, "paralog": false, "identity": 0.9906542056074766, "score": 422.0, "evalue": 1.31e-152, "description": "Internal stop codon at 671405, 671444. Nonsense mutation around 671405, 671444.", "psc": { "uniref90_id": "UniRef90_UPI0029075D7D", "uniref50_id": "UniRef50_UPI0029075D7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029075D7D", "UniRef:UniRef50_UPI0029075D7D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029075D7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029075D7D" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16378.850099999989, "isoelectric_point": 9.222992134094238 }, "id": "GPKLONFDEH_1139", "locus": "GCA000006625_01137" }, { "type": "cds", "sequence": "contig_1", "start": 671498, "stop": 672517, "strand": "+", "frame": 2, "gene": null, "product": "Unique hypothetical", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPU8", "UniRef:UniRef90_Q9PPU8" ], "nt": "TTGGTTTATGAATATCATAATTCTAAAAATCAATTAGTAGGATTTCAAAATTTAGATGGTGGGCATATTTTATTTAAAACACCGGCGTTAAAATCAGCAAACGGTAATTATGAAACAACATTTAAAATTAATGCTGCAATTGTCCAAAAAAATAAACCATTTAAATCTTTTTTTAACTTTGATAAATATAAAGAATTAGGATTTAAGAATAAATCTTATTTTACAAATCCTGTATTAATTAAAAATATAGATCTTTATTCAAAACAAGTTGTTCAAACACTAAACGATCGTTCAAATCAATCAATTGATTTTAAAGACTATCAAAGCACATTTCAAGCCATTGTTGATACACTTGAAACTAACACGATAAATAAAGATTTTTATAGTGTGAATTTACATGTTGATTTTAACACACTATCAACAAAATACCTACTATTTGCTAATTATTTAAAAAATGAAGGTATTGATAAAAACCGTTATATAAATGAATTAAAAATCGATAACTATAAACAGAAGTATTTAGATTTATTTCAAAACCAAGTTTTAATTCCAAAAGAATTAATTGACGATCCAAAAAAAATAGAAGAAGTGCAAAAAATTATTGCAAAAGCCAAAGCCTTGTTAATTGCTAAAATTCGTGAAGAAACAAATATTTTTTATGAAAACTCACAAAAATCAGCTCTTGAAAATAAAAATAAGATTCGTGATTATGAGATGCAATTAAAACAAATAAATATTGCTCTACAAAATTCGAATTATTCTAATAACGAACTTATTCAATTAAAAAATGAACAAAAAGTATTAGCAGCAAATCTCGATCAAGCAAAATTTAAACACTTTTTATATAATGATTTAATTAAACAAGATTTCCGTTCGATTTTAAATAATTATAATATTTATTATCGCAAACATGAATCAGCAATTTATAATGATGTTAAAAGTGATTCAAATTTTATTGTCACAAATGCAAGCAATCCAACATATACACAAAACGGTTTAATTGCACCAAGAAATCAATGA", "aa": "MVYEYHNSKNQLVGFQNLDGGHILFKTPALKSANGNYETTFKINAAIVQKNKPFKSFFNFDKYKELGFKNKSYFTNPVLIKNIDLYSKQVVQTLNDRSNQSIDFKDYQSTFQAIVDTLETNTINKDFYSVNLHVDFNTLSTKYLLFANYLKNEGIDKNRYINELKIDNYKQKYLDLFQNQVLIPKELIDDPKKIEEVQKIIAKAKALLIAKIREETNIFYENSQKSALENKNKIRDYEMQLKQINIALQNSNYSNNELIQLKNEQKVLAANLDQAKFKHFLYNDLIKQDFRSILNNYNIYYRKHESAIYNDVKSDSNFIVTNASNPTYTQNGLIAPRNQ", "aa_hexdigest": "1196aa99424c222e2c0ec8b59bc41020", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 671198, "stop": 672814, "observations": { "mutation": [ 671405, 671444, 672515, 672740 ], "stop": [ 671405, 671444, 672515, 672740 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPU8", "query_cov": 1.0, "subject_cov": 0.41955445544554454, "identity": 0.997, "score": 650.0, "evalue": 3.89e-228, "gene_start": 1, "gene_end": 339, "reference_start": 173, "reference_end": 511, "reference_sequence": "MKKMFLLMKNMMRLFFKNKTLVTQLSILMIVSTVVIVATTITVQRLQKSQSDIKKIGLQSDFLIDTKEQDNINFNEIDVSNNLVKHNEKLIQLATFNQNILKNSNNYLQLVDNAFHNHNLFWPTNVNNQKIDVLNLEGNIDWNKINQLKLQTVFWFENNKENNQNFANNYDDLVYEYHNSKNQLVGFQNLDGGHILFKTPALKSANGNYETTFKINAAIVQKNKPFKSFFNFDKYKELGFKNKSYFTNPVLIKNIDLYSKQVVQTLNDRSNQSIDFKDYQSTFQAIVDTLETNTINKDFYSVNLHVDFNTLSTKYLLFANYLKNEGIDKNRYINELKIDNYKQKYLDLFQNQVLIPKELIDDPKKIEEVQKIIAKAKALLIAKIREETNIFYENSQKSALENKNKIRDYEMQLKQINIALQNSNYSNNELIQLKNEQKVLAANLDQAKFKHFLYNDLIKQDFRSILNNYNIYYRKHESAIYNDVKSDSNFIVTNASNPTYTQNGLIAPRNQWIDNMVQINEKNKNSQNPNKKFKRIFINDDNSNKINNFYDLYSNYIKVIQILKNSYEKDPDTDKDLLVEERDYTPWINTSLINIYQLPQFFNILFNQSEWLLSIQYLKEMSDYKKYLLQIFEPYQDKEWIFNLKHKDWQDGEQVVAIYKNADSNSDQKEYYFNGYVKNQRAYINVKYNKNLPTRLKLINIIKQNQINQKNNLVNVLDQDLNDYAKIINFKKGIKDINTNQQSVYDQWALNNPQDYAMFKIWLSKIIKRYYFLRFSNHLMDIQFKHKTFYQKLCYCRLQLILLIVVQI" }, "paralog": false, "identity": 0.9925650557620818, "score": 1068.0, "evalue": 0.0, "description": "Internal stop codon at 671405, 671444, 672515, 672740. Nonsense mutation around 671405, 671444, 672515, 672740.", "psc": { "uniref90_id": "UniRef90_Q9PPU8", "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PPU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPU8", "UniRef:UniRef50_Q9PPU8" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPU8" ], "product": "Unique hypothetical" } }, "id": "GPKLONFDEH_1140", "locus": "GCA000006625_01138" }, { "type": "cds", "sequence": "contig_1", "start": 672527, "stop": 672742, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCAAATTAATGAAAAAAATAAAAATAGTCAAAATCCAAATAAAAAATTTAAGCGAATTTTTATTAATGATGATAATTCAAATAAAATTAATAACTTTTATGACCTTTATTCAAATTACATTAAAGTCATTCAAATTTTAAAAAATAGTTATGAAAAAGATCCAGATACTGATAAAGATTTACTAGTTGAAGAGCGTGATTATACTCCTTGA", "aa": "MVQINEKNKNSQNPNKKFKRIFINDDNSNKINNFYDLYSNYIKVIQILKNSYEKDPDTDKDLLVEERDYTP", "aa_hexdigest": "f9025ea7460b2dd651f8f36f57c1c660", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8575.456299999998, "isoelectric_point": 6.181035804748535 }, "id": "GPKLONFDEH_1141", "locus": "GCA000006625_01139" }, { "type": "cds", "sequence": "contig_1", "start": 672944, "stop": 673225, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTGCTATTTATAAAAATGCAGATAGTAATAGCGACCAAAAAGAATATTATTTTAACGGCTATGTTAAAAATCAACGAGCTTATATTAATGTTAAATATAATAAAAATCTGCCAACACGTTTAAAATTAATTAATATTATTAAACAAAATCAAATAAATCAAAAAAACAATTTAGTTAATGTTTTAGATCAAGATTTAAATGATTATGCAAAGATTATTAATTTTAAAAAAGGTATTAAAGATATTAATACAAACCAGCAATCAGTTTATGATCAGTGA", "aa": "MVAIYKNADSNSDQKEYYFNGYVKNQRAYINVKYNKNLPTRLKLINIIKQNQINQKNNLVNVLDQDLNDYAKIINFKKGIKDINTNQQSVYDQ", "aa_hexdigest": "ab041729b83b96d8f53d3b5ff45f62b3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10991.292999999989, "isoelectric_point": 9.522447776794433 }, "id": "GPKLONFDEH_1142", "locus": "GCA000006625_01140" }, { "type": "cds", "sequence": "contig_1", "start": 673360, "stop": 673578, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FE7A4E", "UniRef:UniRef90_UPI0002FE7A4E" ], "nt": "TTGTTATTGCCGTTTACAATTAATATTATTAATCGTAGTTCAGATCTAGCATACGTAAGCACATCTTATATTAAAGCTAATCAAAGAACAACAAAAAATATAAATAATTATAAAGAAATTGGTAATTATGAAGATTTTTTACAAGCACTAAAACTTCCATATCGTATAATCGATGATCAACCAAACTATCGTGAAATTTACTTTGCCAAACGGTCGTAA", "aa": "MLLPFTINIINRSSDLAYVSTSYIKANQRTTKNINNYKEIGNYEDFLQALKLPYRIIDDQPNYREIYFAKRS", "aa_hexdigest": "d9fbe90a39e8b8045ab034c4fd80de7b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002FE7A4E", "query_cov": 0.9722222222222222, "subject_cov": 1.0, "identity": 0.986, "score": 142.0, "evalue": 2.7e-43, "valid": true, "uniref50_id": "UniRef50_UPI0002FE7A4E" }, "pscc": { "uniref50_id": "UniRef50_UPI0002FE7A4E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FE7A4E" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8568.640699999996, "isoelectric_point": 9.299773979187012 }, "id": "GPKLONFDEH_1143", "locus": "GCA000006625_01141" }, { "type": "cds", "sequence": "contig_1", "start": 673805, "stop": 674140, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAATTTTAGAGTATTACACACTAAAAGTTAATACTAATGATCTTAATTTATTACAAAAAAATAATGTTTATAATGAAATAAAATCAGAATTATTAGATTCTAAAAAAGGTAACATATATAAGAGAACTGATAAGGATAACCCTTATCCAATTTATGTTAAACGAACCTTTTTTTTAACACAATTGAGGAATTTAATTATTGCAATTGCACTTAGTTTGATTGTTTTAATCTTATTGTTAGGAATCTACTTTGTTACCTCAAGTATTAAAAATATGATTAATAAAAACAAAGTCATGTTCGGTGCTATGCAAGCTCAAGGCGTATCAAGATGA", "aa": "MPILEYYTLKVNTNDLNLLQKNNVYNEIKSELLDSKKGNIYKRTDKDNPYPIYVKRTFFLTQLRNLIIAIALSLIVLILLLGIYFVTSSIKNMINKNKVMFGAMQAQGVSR", "aa_hexdigest": "54f1f352651a3ef0b962abbbba68d02d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12833.063999999988, "isoelectric_point": 9.801338005065915 }, "id": "GPKLONFDEH_1144", "locus": "GCA000006625_01142" }, { "type": "cds", "sequence": "contig_1", "start": 674351, "stop": 675214, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "GTGTTAATTTATTATATTTTAAAACATTCAACCGCAGAGACAATGAAAGGCGATGCAGGTTTTAAAATCAATAAATTTATCATTAAAACAAAAGCAATCTTTTTAAAATTTGGTGCAATTCGAGTTTTAAAAGCTACTTATGTTGTTGCCAATTTAGCAAGAATGTTGATTTTATTAGTAATCGCTTCCTCTTTTTCTATTATTGCTTCTATTATTGCAACAACAAAAAATAATTTTAGTCAGTCAGCAAGTATTACTAATGCTAAACGTGAATATCAATATGCAGTTGATTTATATTCACCAACAGAACAGGGAGGATTTTATTACATTTCAAAGTTTGATGAAATGGGTGTTTCTAATGAATATGCTCCTTTAAAACAAATTGTTAAAAATGTTAAGATTGTAATTAAAATTAAAAATTTAGTTAATAAAAAAGTCAATGTTGTATATATCAATAATCAAACAAAGCAAAAAGTAATTATTGAAGCACTTATTAATGATAAGGGAATAATCACTTTTAAAACTAATCAGCTTGTTAAAGGTAGTTTTCAATTTGAAGGTATTTATGAACAAGAAACAAATAGAGTTTTAATTAATTCACAAAATTTAAATCCATCATTATTAAATATAGATAATCGAAAGATAGGCCATTTTATTTACTCTAATCAAAAACAATATATCTATTTACTAAAATCATACTTTAATGACAATGATGTAATTTTAGATTATGTTGATGCTAATAATCAACATACTTATTTAAAAGCAAAAACTCTAGGTCAATACTTGGTATTTGATCCTTCACAATTAAAACAAAAACAAAAATATCAATTAGTTAATGTTATAAACCATAAAGGAAATACATGA", "aa": "MLIYYILKHSTAETMKGDAGFKINKFIIKTKAIFLKFGAIRVLKATYVVANLARMLILLVIASSFSIIASIIATTKNNFSQSASITNAKREYQYAVDLYSPTEQGGFYYISKFDEMGVSNEYAPLKQIVKNVKIVIKIKNLVNKKVNVVYINNQTKQKVIIEALINDKGIITFKTNQLVKGSFQFEGIYEQETNRVLINSQNLNPSLLNIDNRKIGHFIYSNQKQYIYLLKSYFNDNDVILDYVDANNQHTYLKAKTLGQYLVFDPSQLKQKQKYQLVNVINHKGNT", "aa_hexdigest": "6681d4d1855ceb8d6a92e6b89934a892", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 57, "aa_cov": 0.1951219512195122, "hmm_cov": 1.0, "evalue": 3.3846297452044913e-10, "score": 35.800994873046875, "start": 231, "stop": 286 } ], "seq_stats": { "molecular_weight": 32871.78610000003, "isoelectric_point": 9.698124122619632 }, "id": "GPKLONFDEH_1145", "locus": "GCA000006625_01143" }, { "type": "cds", "sequence": "contig_1", "start": 675251, "stop": 676096, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07198.17" ], "nt": "TTGGATTATTCCAAAAATGGACGTTATTTATATGATTATTATGGAAGATTACCATATTTATACTTTAATCATTTACCACAAAATCTAATTAACCAAAAAGTTTATTTGACTTATACAATTAACCAAAATGATGAATTTCGAATTTATGGTATTGTTAATAAAGAAAAAGAAGTAGTTTTTGACTTAACAAAATTAAAAATTAATACCCAACAAAGTTATCAATTACGCGATATGCGAATCGCAAATAGTGATTTATTAATTTATGATAATAAACAACATGAATTTAATAATAATATTTTATATTTTGATAACAATTATTACGGTTTAAACAATAATAATCAAAAGCAAATTAATATTGATTACTTAACTCCTAATCGTAATCTTTATTTAACTTTAAAAGCAATGGGTAATGAATTTGTTAATAAGAAAAAAATACCTTTTATAATGACAAAAGCAATGGCTTCTTTAGTTGAACCTGATACTGCAATTTTAGATTATAATGAATTAGAAAAGGGTTATGTTTATCAAATTAGTACAGCAACACTAGAGGGTAGTGATACAAACTTATACTTAGATAACGTTAATTCTATTGATAAAACAAAAGATAATGGCTTACCTTATTCGTCTCTAAAATACAATAACGCAATGCGTGAAATTAAAAATTTAGGTACTGATCATCCAGTCTATGGCAAAATGTTATCAAATGTTTATTATCCATCAATTAGTACCCAACCTGAAATCTTACAAAATATTCGTTTTTTTGATAGTAAAGTAATTTCAAAAAGTTCTTTAGATGGAAATATTAGTGTTAAAGCAACGGATGTTAACATTGAATTTAACCCTTGA", "aa": "MDYSKNGRYLYDYYGRLPYLYFNHLPQNLINQKVYLTYTINQNDEFRIYGIVNKEKEVVFDLTKLKINTQQSYQLRDMRIANSDLLIYDNKQHEFNNNILYFDNNYYGLNNNNQKQINIDYLTPNRNLYLTLKAMGNEFVNKKKIPFIMTKAMASLVEPDTAILDYNELEKGYVYQISTATLEGSDTNLYLDNVNSIDKTKDNGLPYSSLKYNNAMREIKNLGTDHPVYGKMLSNVYYPSISTQPEILQNIRFFDSKVISKSSLDGNISVKATDVNIEFNP", "aa_hexdigest": "9b525332d887912be1b10ec0283129da", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07198.17", "name": "DUF1410", "length": 59, "aa_cov": 0.2099644128113879, "hmm_cov": 0.9661016949152542, "evalue": 1.574766365638983e-12, "score": 43.27106475830078, "start": 26, "stop": 84 } ], "seq_stats": { "molecular_weight": 32850.65050000005, "isoelectric_point": 6.328703498840333 }, "id": "GPKLONFDEH_1146", "locus": "GCA000006625_01144" }, { "type": "cds", "sequence": "contig_1", "start": 676373, "stop": 677095, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACAGAGCAGGGCGCCGCAGAATTCTTACCAGAATTTTTACGTTTAATTACTCAAATGTACACCTTCAAAACAACTCGTTATTTACAATATAAAGCCGGTTTAAAATTTGTTAATATTGAAGATGAAAAAGTACAACAACCATATACATACTTATTAGGTAAAATGCAAACAAAAACAAAAAGATTTGCTAATGAAATTAAAATATTGGGTATTAATTATGAAAAAAATAAATTTATTAATGGAAAACCAAGTCCTACTACTCAACTAAGTTTGATAAATGATCAGGGTGTTAATTTAATTGATCTAATTAAAAATGATGATCCTAATAATGAGATTCACAACATTATTATTAATAAAGTTGTCGCCCAAAAATATCATTTAAAGATTAATGATGTTTTTGAATATCAAATTAATAATCACATCAAACGTCTATCAAATAAATTTAATAACACACCAGAGAATTATAAAACTAAGTTTAAAGTTGTTGGGATTAATGATAGTTTAATGGATGAGCAATTAATTATTAATCAAAAAGTTGCAAATAAACTAATTGGTTTTGATGATTATCGCCGTCGGATTCAAATCGAAAATGGTTATCCAATCATTGAAGAACGCGAGTTCAATGGATTCTTTACAAAAGAAAAAGAACCTATTTTCTTTAATAATATGGCTTCATTTTATTCACCTACTGGACTATCACCAGCAATTGATTCATGA", "aa": "MLTEQGAAEFLPEFLRLITQMYTFKTTRYLQYKAGLKFVNIEDEKVQQPYTYLLGKMQTKTKRFANEIKILGINYEKNKFINGKPSPTTQLSLINDQGVNLIDLIKNDDPNNEIHNIIINKVVAQKYHLKINDVFEYQINNHIKRLSNKFNNTPENYKTKFKVVGINDSLMDEQLIINQKVANKLIGFDDYRRRIQIENGYPIIEEREFNGFFTKEKEPIFFNNMASFYSPTGLSPAIDS", "aa_hexdigest": "fb059554fb6f015b99b10ab7ef9b70b4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28080.82330000002, "isoelectric_point": 8.959767723083495 }, "id": "GPKLONFDEH_1147", "locus": "GCA000006625_01145" }, { "type": "cds", "sequence": "contig_1", "start": 677161, "stop": 677418, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGTGATGGAATAATAGCAGTAATGATATTTAATAATAATGTAAATGATGTTTTATCAACTACTGAAGCAAATATTTCGTTTTCAATATTAGCATCAACTGCTTTAAATGATGGTAAATTAGCTTCTTTTCCAAAAATTTTTCCTATTTTCTTTAATAATTTATCTAAATTAATTTCTGAAAAAGGTTCTCAAGTAATTTTATTTTTATCGAAACTAGCATTTAAAATCGAATTAGCTAAATCTCAACTGTAA", "aa": "MTSDGIIAVMIFNNNVNDVLSTTEANISFSILASTALNDGKLASFPKIFPIFFNNLSKLISEKGSQVILFLSKLAFKIELAKSQL", "aa_hexdigest": "1b881d439b7343f32eedc804b5aa7c3d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9254.716299999991, "isoelectric_point": 8.064882087707517 }, "id": "GPKLONFDEH_1148", "locus": "GCA000006625_01146" }, { "type": "cds", "sequence": "contig_1", "start": 677822, "stop": 677953, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02637.23" ], "nt": "ATGTTATTACAATACAATGAACGCCCCGACCGTGTTGAAAAATTTTTTATGGGTGAAATAATGAAATTAACTAAAGCACAAGCGAATCCAACCATTTCTTTTAATGTTTTAAAAAAAATCTTACAAAAATAA", "aa": "MLLQYNERPDRVEKFFMGEIMKLTKAQANPTISFNVLKKILQK", "aa_hexdigest": "ebd6cc6780bc1ef791e1502f5518549e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02637.23", "name": "GatB_Yqey", "length": 40, "aa_cov": 0.9302325581395349, "hmm_cov": 1.0, "evalue": 5.3898482211391504e-11, "score": 38.17214584350586, "start": 2, "stop": 41 } ], "seq_stats": { "molecular_weight": 5113.073299999999, "isoelectric_point": 9.871673011779784 }, "id": "GPKLONFDEH_1149", "locus": "GCA000006625_01147" }, { "type": "cds", "sequence": "contig_1", "start": 677981, "stop": 678214, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02637.23" ], "nt": "TTGGAATTTATTGGTCTAATTAATAAAAATACGCAAAACATTGAAGAAATTACACCAGAATTATTGCAAAAAATTGCTAGAATGATCTTATTATTTGATCAAACTTTGATTAATGGAAAACAAACAAAAATAATTTTAGAAAAAATTTATTTGACAAATAAAGATCCTCAAATTCTAATTAAGGAATTAGGTTTTGAGCAAATTACTAATGAAAATGAAATTACTAAACTTTGA", "aa": "MEFIGLINKNTQNIEEITPELLQKIARMILLFDQTLINGKQTKIILEKIYLTNKDPQILIKELGFEQITNENEITKL", "aa_hexdigest": "6be684dd56d29286213497a78edf8c05", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02637.23", "name": "GatB_Yqey", "length": 78, "aa_cov": 0.987012987012987, "hmm_cov": 0.9615384615384616, "evalue": 2.338990339237275e-13, "score": 45.83795928955078, "start": 2, "stop": 77 } ], "seq_stats": { "molecular_weight": 8999.489699999995, "isoelectric_point": 5.05215663909912 }, "id": "GPKLONFDEH_1150", "locus": "GCA000006625_01148" }, { "type": "cds", "sequence": "contig_1", "start": 678221, "stop": 678739, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "TTGCGAAAAATTTTATTGTTTAATGATATATCAGATGCAAAAATGGAAGATGGATCATTACGTGTTGATGTAAACGTTTCAATTAGACCTCAAGGGGCTAAAAAATTTGGCACAAAAGTAGAAATTAAAAATATTAATTCTATTTCAAATGTTGCTAAAGCTATTGATTATGAGATAAGACGTCAATTAAATTTAATTTTACTAAATCAGAATGTTGAACAACAAACACGACGTTTTGATGATAATACGAATACAACGGTTTTTATGCGTTCAAAAAATGATGCTATTAATTATCGCTATATTCGTGAAGCAAATATTGCACCAATTCATCTAAGTGATGATTATGTAAAAAAAATGTTTTTAACTAAATCATGTAGTATTAATGATTTGCGTCAACAACTAGCACAAAAAGGTTTAGTTAGTTCTGCTATCGAACAGTTATTAAGTGATGGACCGTTGTTTAAAGCATTTAAATATGTAGACAAAATTGTTAATAATCCTTTAAGTGTTTATAAATGA", "aa": "MRKILLFNDISDAKMEDGSLRVDVNVSIRPQGAKKFGTKVEIKNINSISNVAKAIDYEIRRQLNLILLNQNVEQQTRRFDDNTNTTVFMRSKNDAINYRYIREANIAPIHLSDDYVKKMFLTKSCSINDLRQQLAQKGLVSSAIEQLLSDGPLFKAFKYVDKIVNNPLSVYK", "aa_hexdigest": "9b3cba2c635531bfd2394b70b89ae4d9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 116, "aa_cov": 0.6744186046511628, "hmm_cov": 0.9396551724137931, "evalue": 2.70666844974391e-40, "score": 133.84681701660156, "start": 1, "stop": 116 } ], "seq_stats": { "molecular_weight": 19744.501199999988, "isoelectric_point": 9.590268516540526 }, "id": "GPKLONFDEH_1151", "locus": "GCA000006625_01149" }, { "type": "cds", "sequence": "contig_1", "start": 678809, "stop": 679255, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "ATGCAAAATTTTGAAGTTATTATTGGTATTGAAGTGCATACTGCACTTAATACAAAAACCAAAATGTTTTCTAATGCAGCAACATCGCATAAGAGTATTCCTAACACTTTAATTAATGAAATTGATTTAGCATTGCCAGGTACATTACCAACTGTTAATCAAGAAGTTGTTCATAAAGGACTTTTTTTAGCAAATGCATTACACATGCGTACTAATCATCAATTTATTGCTTTTGATCGTAAGCATTATTATTATTTAGACTTACCTAAAGGATATCAAATTACACAAAATTATTTTCCAATAGGTCAGAATGGTTATATTCAAATCATTGATGAATATAATAATTTAAAACGAATACGTATCAAACAAATTCATTTAGAAGAAGATACAGCCAAACAAACTAATATAGGTAATCAAATATATTTAGATTATAACCGTGCAGGATGA", "aa": "MQNFEVIIGIEVHTALNTKTKMFSNAATSHKSIPNTLINEIDLALPGTLPTVNQEVVHKGLFLANALHMRTNHQFIAFDRKHYYYLDLPKGYQITQNYFPIGQNGYIQIIDEYNNLKRIRIKQIHLEEDTAKQTNIGNQIYLDYNRAG", "aa_hexdigest": "dbc55417259b7264cfe0ab47a4c457d2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 145, "aa_cov": 0.9662162162162162, "hmm_cov": 0.9862068965517241, "evalue": 2.3271450344438814e-51, "score": 170.1444854736328, "start": 6, "stop": 148 } ], "seq_stats": { "molecular_weight": 17090.25789999999, "isoelectric_point": 7.9748842239379885 }, "id": "GPKLONFDEH_1152", "locus": "GCA000006625_01150" }, { "type": "cds", "sequence": "contig_1", "start": 679336, "stop": 680691, "strand": "-", "frame": 1, "gene": "gatA", "product": "Glutamyl-tRNA amidotransferase subunit A", "db_xrefs": [ "COG:COG0154", "COG:J", "SO:0001217", "UniRef:UniRef50_A0AAP9ADD2", "UniRef:UniRef90_Q9PPU5" ], "nt": "ATGAAAAATAAAACTATTTTAGCGCTTCATAATCAAATTAAAAAACGTGAAGTTAAAATTTATGATTTGATTAAAAAATCGATTGATCATGATTTAAAGATTAAAAATTTAAATGCAACTTTAGCAAATAATTATGAACAGGCATTGAAACAAGCAGCTTTTTTAGATGAAAAGATTAGTAGTGATAATTTAGAAATTGATTATTTATTTGGAATTCCATATTCTTTAAAAGACAACATTTCAACTAAGAATATTATTACAACAGGTGGTTCATTGTTTTTAAAAAATTACAATCCTCCATATGATGCGACAGTTAAAACATTATTAGATCAAAAACAAGCAATTTTGTTAAATAAATCAAATTTAGATGAATTTGGTTTAGGAGGCACTGGTACTGATTCAGCATTTGGCCCTGTAATTAATCCATTAAATCCAAAACATGTTAGCGGAGGTTCTTCATCTGGTAGTGCTGTTTTAGTTCAACAAGGCGTTGTACCTTTTGCAATTGCTACAGATACAGGAGATTCTGTTCGTCGCCCCGCTTCAATTATGGGTATTGTTGGTTTTAAACCGTCATATGGTCTTATTTCGCGTTATGGCGTTTACCCCTTTGCTCCTTCTCTTGATCATGTTGGAATTTTTACAAATAATATTTTTGATACGCAAATTGTTTTTAATGCTCTTGCTAAGCGAGATTTTAAAGATTTTACAAGTCTTAGTTTTAAAACACAATCACTTTCAATGCTAAAAACAAAAAATAAATATAAGATTGGTGTTTTAAAACCTAGTTTTGATTTATTAGCATCAAAATATCAACAAGAATTAACTAATCTGATTGTTAAATTAAATGATGAGCATGAAATAATTAATGTTGATTATGATATTAATTTATTAAAGGCAATTAGTCCAGTTTATAAATTAATCGCTTTTAGTGAGGCTTATAGTTCATACTCTAATTTTACAAACATCACTTTTGGCAAACATGATATTGATTATGATAACTATGAGGATTTAATTATTAAAACGCGAACTTTATATTTAGGAACACAATTAAAACAACGTTTTATGATAGGTGCTTATGTTACTCAAGAAAGCCAGTTTGAAACTTTATTAGTTGCTGCTAAAAAAATGCGTACATTAATAGTTGATCAAGCTAAAAATTTATTTAAAAATTGTGATTTAATTTTAAATTTAGCAGTTCATGATATAAGTGAAACAATTGATGATGTTAACAACCAAAAGCCCACAACTAATTTAGTTGAAGATGTAATGCAAATTGCTAATTTTGGTGGTTTTCCATCAATTGTAATCCCCTTTTTAACAACTAAATTAGGAAATTTAGGTTTAAATTTGTGA", "aa": "MKNKTILALHNQIKKREVKIYDLIKKSIDHDLKIKNLNATLANNYEQALKQAAFLDEKISSDNLEIDYLFGIPYSLKDNISTKNIITTGGSLFLKNYNPPYDATVKTLLDQKQAILLNKSNLDEFGLGGTGTDSAFGPVINPLNPKHVSGGSSSGSAVLVQQGVVPFAIATDTGDSVRRPASIMGIVGFKPSYGLISRYGVYPFAPSLDHVGIFTNNIFDTQIVFNALAKRDFKDFTSLSFKTQSLSMLKTKNKYKIGVLKPSFDLLASKYQQELTNLIVKLNDEHEIINVDYDINLLKAISPVYKLIAFSEAYSSYSNFTNITFGKHDIDYDNYEDLIIKTRTLYLGTQLKQRFMIGAYVTQESQFETLLVAAKKMRTLIVDQAKNLFKNCDLILNLAVHDISETIDDVNNQKPTTNLVEDVMQIANFGGFPSIVIPFLTTKLGNLGLNL", "aa_hexdigest": "a703320b57be2f2836b7b529a0b799f8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PPU5", "query_cov": 1.0, "subject_cov": 0.9454926624737946, "identity": 0.998, "score": 875.0, "evalue": 0.0, "valid": true, "gene": "GatA", "product": "Glutamyl-tRNA amidotransferase subunit A", "uniref50_id": "UniRef50_A0AAP9ADD2", "cog_id": "COG0154", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9ADD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9ADD2" ], "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA" }, "genes": [ "gatA" ], "id": "GPKLONFDEH_1153", "locus": "GCA000006625_01151" }, { "type": "cds", "sequence": "contig_1", "start": 680681, "stop": 680965, "strand": "-", "frame": 3, "gene": null, "product": "Glu-tRNAGln amidotransferase C subunit", "db_xrefs": [ "RefSeq:WP_006688729.1", "SO:0001217", "UniParc:UPI00000C1CB4", "UniRef:UniRef100_A0AAC9X7P1", "UniRef:UniRef50_A0AAP9AD84", "UniRef:UniRef90_A0AAC9X7P1" ], "nt": "ATGAGTTATAATTTACAAAAATTAATGTCATCATGCATGTTTATATTAACTGATGATGAAATTAAACAGGTTGAAAATCGTTTAAATAATTTATTTAATGAAATTAAAGTTTTTGAGCTTTTTAATTTACAAAATATAACACCTTTTGAAATTATTAATAAATCCTTTGATAATTTTTTACGAGAAGATGAAATTAATTCTTGTGTAGGTAATAATAGCGAAGAAGTTCTAAATAATTGCCTAAAAGTAATCGATCATTATGGGGTATTAAAAAATGAAAAATAA", "aa": "MSYNLQKLMSSCMFILTDDEIKQVENRLNNLFNEIKVFELFNLQNITPFEIINKSFDNFLREDEINSCVGNNSEEVLNNCLKVIDHYGVLKNEK", "aa_hexdigest": "157da448b653e48039d75fec505f2a22", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1CB4", "ncbi_nrp_id": "WP_006688729.1", "uniref100_id": "UniRef100_A0AAC9X7P1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CB4", "RefSeq:WP_006688729.1", "UniRef:UniRef100_A0AAC9X7P1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X7P1", "uniref90_id": "UniRef90_A0AAC9X7P1" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X7P1", "product": "Glu-tRNAGln amidotransferase C subunit", "uniref50_id": "UniRef50_A0AAP9AD84" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9AD84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9AD84" ], "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC" }, "genes": [], "id": "GPKLONFDEH_1154", "locus": "GCA000006625_01152" }, { "type": "cds", "sequence": "contig_1", "start": 680986, "stop": 681105, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGTTTTAAACTTATTGATTATTTATCCAATTTTTAAAATTGTTGTTCCTTTAATTCGTTATGATTATGAAGATGATATTGTTGAGGATAAACATATTCCAATTCATGTTGACTAA", "aa": "MVVLNLLIIYPIFKIVVPLIRYDYEDDIVEDKHIPIHVD", "aa_hexdigest": "ce7815c80289df7ad8446bfc50f87c58", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4664.504000000001, "isoelectric_point": 4.684465217590332 }, "id": "GPKLONFDEH_1155", "locus": "GCA000006625_01153" }, { "type": "cds", "sequence": "contig_1", "start": 681289, "stop": 681636, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGATTTATTATCTGTGGCTATGACTGCTGGTGTATTTCATTATGGTTATTTAATTGCTGCTATGGCTTATGGATTAATTGGAGGACTAATTCGAATTATTCTAACTACTTCCAAAAAAAAGGATTTACCATTTGCAATTTATAGTTCAATGGCAACTATTTTAATTGGTGTAGGAATTGCCTTATTTTTATATTTTGCTCCAGGAATTGGTGATAAGGGTTTTAATATTCAATTATTTGGTTTTGATATTAAAATTGCACGAACATTGATGATTGGCATTATTTTAGGTTTTTTCTTATTATCAATTATCGTTGTTTGGTTTAGTTTATTGATAAAATATTGA", "aa": "MTDLLSVAMTAGVFHYGYLIAAMAYGLIGGLIRIILTTSKKKDLPFAIYSSMATILIGVGIALFLYFAPGIGDKGFNIQLFGFDIKIARTLMIGIILGFFLLSIIVVWFSLLIKY", "aa_hexdigest": "6357ca13e61cb2890a9b3c2ba04aaa36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12577.200399999983, "isoelectric_point": 9.522254371643069 }, "id": "GPKLONFDEH_1156", "locus": "GCA000006625_01154" }, { "type": "cds", "sequence": "contig_1", "start": 681724, "stop": 681921, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTTTTTATTATCTTTACAGTTATTAAAAAAATCTATTATTCAATTCGTTTTCAAAAGCGTTTTTATATTATTCCTAAAACTTCGGTTAAAGGGATTTCAAATATTGCTATGGTGATTTCGATTTCTGTTGCAGTGATTATATTATTAACAGTTTTATCAGCTAACACAGCTTCTGTAATTTTTCGTGCTTGA", "aa": "MIVFIIFTVIKKIYYSIRFQKRFYIIPKTSVKGISNIAMVISISVAVIILLTVLSANTASVIFRA", "aa_hexdigest": "f3e60583b3fd2f6615fa308ea2dc542d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7324.946399999999, "isoelectric_point": 10.76797695159912 }, "id": "GPKLONFDEH_1157", "locus": "GCA000006625_01155" }, { "type": "cds", "sequence": "contig_1", "start": 682044, "stop": 682199, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTACATTAATTTGATTGATCAAAAACAAAATTTAATTTTATGTGCTTCACATCCTTCGCCATTAAGTGCACATCGTGGTTTTTTAACTTGTAAACATTTTAGTAAATGCAATGATTATTTAATTAAACATTTACGCACTCCAATAAAATGA", "aa": "MSYINLIDQKQNLILCASHPSPLSAHRGFLTCKHFSKCNDYLIKHLRTPIK", "aa_hexdigest": "b1b830235f3ee58402e16c842af1e7b5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5895.903299999999, "isoelectric_point": 9.508716011047362 }, "id": "GPKLONFDEH_1158", "locus": "GCA000006625_01156" }, { "type": "cds", "sequence": "contig_1", "start": 682212, "stop": 682460, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTAGGAAATAATTTACCAGGATCACTAATCAATATTTTTAAAGCTTTAGAATATGATTTACAAATAAAAAGAACAAACCCTGATTTGTCTGATTGGGCAAAACAAGGAGTTTTATTGTTGAATACTGTTTTAACTGTTAACGCTCATCAACCAAATTCGCATAAAAATTTTGGTTATGAAGAATTAATTAAAAATGTGTTTAACGAACTTAGAAAACAAAAGCATGTTGTTTATTTATTATGA", "aa": "MDLGNNLPGSLINIFKALEYDLQIKRTNPDLSDWAKQGVLLLNTVLTVNAHQPNSHKNFGYEELIKNVFNELRKQKHVVYLL", "aa_hexdigest": "72bbd58da2aad1582ea9d64ab4766bf8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9446.756899999998, "isoelectric_point": 8.044703483581543 }, "id": "GPKLONFDEH_1159", "locus": "GCA000006625_01157" }, { "type": "cds", "sequence": "contig_1", "start": 682676, "stop": 682807, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCTTTAGAAAATAAGGATGCTAATATTCATAATTTGACTAGTTTTAAAGCTGCTAAGAATAATTTTAGAAAATATCTTTTTAACCTTAATGAAATTAAAAGTGTAAATGTTAACTATAGCAAATGA", "aa": "MIALENKDANIHNLTSFKAAKNNFRKYLFNLNEIKSVNVNYSK", "aa_hexdigest": "6b8507937fe3024ff3529f407df478eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5016.689300000002, "isoelectric_point": 9.777226829528807 }, "id": "GPKLONFDEH_1160", "locus": "GCA000006625_01158" }, { "type": "cds", "sequence": "contig_1", "start": 683021, "stop": 683449, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCTAGTTCTAATGGTAGTCAACTAAATGCACTTAGTTCAATTAACGCTGCTTTATCAAGTGTTGATAAAAAAACTTATGCTGAAATTCATGATGATCACAAACCAGAAATTCGTAATTTAGTTTATGAAATTCCATTAAAAGATATGGTAGTTAATTTTGCACCATCAACAATGCGTTCTGCTAATATTAATCAAAAAGCAACACAAACTCTTCAAGAAAATTTTTTAAAAATTAAAAATCAAAAAACAGGGTTAACTGAGACAGAGAAAAAACGTACAACTGTTTATAATGAATACTACACAGGTACATTATCTAAAATTCATGCGTTTAGTATTTTGGGCCGCGATTTAAAACAAGAATTAAAAAACGAAGCTAAAGTTAATAAAAAAGAGTATGTTGAAGATAAAAAACTTGAAGACTGA", "aa": "MEASSNGSQLNALSSINAALSSVDKKTYAEIHDDHKPEIRNLVYEIPLKDMVVNFAPSTMRSANINQKATQTLQENFLKIKNQKTGLTETEKKRTTVYNEYYTGTLSKIHAFSILGRDLKQELKNEAKVNKKEYVEDKKLED", "aa_hexdigest": "8d115398ba4d34f55b200d6eeaa24375", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16168.075799999988, "isoelectric_point": 8.650577354431153 }, "id": "GPKLONFDEH_1161", "locus": "GCA000006625_01159" }, { "type": "cds", "sequence": "contig_1", "start": 683466, "stop": 683798, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGAAGAGTTATATCAAGCATATTGTAATTTAATATTTTTTAAACGTACAAGAACATTTTTTAAACTTGGATTAACTAATAATTCATCATTTACTTTAACACCAAAATTAGGTTTATTAATACTTAAACCTGTAATTGCATCTACATATTCTCAATCAAAACTAAAATTATATAAAGCAAGTTTGTATGTATGTTTACCTGCACCTACACCTTGTACTAAAGCTAATAAAAATTCTTTAGCAACTTCGTTTTTTAACCCTTTAAAAAGTTCAAAATCTTTATTTGAATGGTTATTAAATGCATGTTCTGCTAATCATTTTATTAATTGA", "aa": "MFEELYQAYCNLIFFKRTRTFFKLGLTNNSSFTLTPKLGLLILKPVIASTYSQSKLKLYKASLYVCLPAPTPCTKANKNSLATSFFNPLKSSKSLFEWLLNACSANHFIN", "aa_hexdigest": "dc3ae9fe768a38317f680e55d7ba59ed", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12473.53549999998, "isoelectric_point": 9.733001518249512 }, "id": "GPKLONFDEH_1162", "locus": "GCA000006625_01160" }, { "type": "cds", "sequence": "contig_1", "start": 683783, "stop": 684031, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTATTTTGCAAAATGAATTGATTCGTTTAATTGGTAATAAGTTAAATTTTGATTCAACAAGTAAATATGCTAATAATTTAGAAATTCTTGACCAAACAAAAAAACAAACTGTTTTTAACCAAAAACTATATTCAAGTGATGAATTAACAAAAATAAATAAGGTTAGTGAATTAATCAACTCTTTAGTTATTAGTGATGGATTGTATACTTATATTACCTCTATGACAGATCAATTAATAAAATGA", "aa": "MSILQNELIRLIGNKLNFDSTSKYANNLEILDQTKKQTVFNQKLYSSDELTKINKVSELINSLVISDGLYTYITSMTDQLIK", "aa_hexdigest": "6ce614afe25045b7e8ce1c6bec5fbf30", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9404.677699999998, "isoelectric_point": 6.139941215515137 }, "id": "GPKLONFDEH_1163", "locus": "GCA000006625_01161" }, { "type": "cds", "sequence": "contig_1", "start": 684074, "stop": 684424, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAACAAAAGTAAAAAGCTAATTTTTTCAATATTAGGAATTAGTTTTATTGGATTAACGCCAATTGTTGCTGCATCGTGTTCAGCAGCTGCTCATCCTCAAGTTAATGTTCATTATGTTAGTGCTAATGCTACAACTAATAATATTGGTAAAATTAAAATTCGAGCCTTTGAAGGTAAGAATCAAAAACATGAAATGATTTTTGAGAATCAAAATTTATTAGATGATGTTTATTATGCGGCAGGTGATTTAACTTATACGAGTGTTAAAGATAATAAACGCTTTGATAAAAACAATAAAGAAATCGATCAATTTGGTTATTTGGTTCCCAAAAAAGAAGTTTGA", "aa": "MKKNKSKKLIFSILGISFIGLTPIVAASCSAAAHPQVNVHYVSANATTNNIGKIKIRAFEGKNQKHEMIFENQNLLDDVYYAAGDLTYTSVKDNKRFDKNNKEIDQFGYLVPKKEV", "aa_hexdigest": "d4cbb779ac64bf7060776ff86308997a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13008.792799999988, "isoelectric_point": 9.433545875549317 }, "id": "GPKLONFDEH_1164", "locus": "GCA000006625_01162" }, { "type": "cds", "sequence": "contig_1", "start": 684427, "stop": 684879, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03796.21" ], "nt": "TTGGTTTCAGGAGGCAATGCAAATAAAAATATGACAAGAACACAAGAGGTAGGTCGTGTTTCAAGTGCATTAAAAATCATTGCTAAAGAAATTAATGCACCAGTAATCGCTATCGCTCAACTTTCACGTAAAGCAGAAGAACGTGATGTAAGCAATAATGTTAATATGAAAAATAACCCTTTAGTAAAAACAATTGATAATTCCCCAAAATTGTCTGATTTGCGAGAATCTGGATCAATTGAACAAGATGCTGACGTTGTTGCTTTTTTACATTGGGATCGAAAACAACGCAATTTTATGCAAAATGATAATCAAGAAACCCGAATGCGCGATGATTTGATTGAAGCAAAATTTATTGTTGAAAAGAACCGAAACGGGTCAACAGGTGAAACTGATATTATTTTTTCTAAATTAAATAGTAAATTTATTCGTGCAACCACTTCAAAGGAGTAA", "aa": "MVSGGNANKNMTRTQEVGRVSSALKIIAKEINAPVIAIAQLSRKAEERDVSNNVNMKNNPLVKTIDNSPKLSDLRESGSIEQDADVVAFLHWDRKQRNFMQNDNQETRMRDDLIEAKFIVEKNRNGSTGETDIIFSKLNSKFIRATTSKE", "aa_hexdigest": "f3b7b5a7006f7426c6c5783386b56edc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03796.21", "name": "DnaB_C", "length": 137, "aa_cov": 0.9, "hmm_cov": 0.7956204379562044, "evalue": 1.369196273131017e-32, "score": 108.67457580566406, "start": 8, "stop": 142 } ], "seq_stats": { "molecular_weight": 16893.849299999998, "isoelectric_point": 9.256193351745605 }, "id": "GPKLONFDEH_1165", "locus": "GCA000006625_01163" }, { "type": "cds", "sequence": "contig_1", "start": 684991, "stop": 685677, "strand": "-", "frame": 1, "gene": "dnaB", "product": "DNA 5'-3' helicase", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_Q9PPU0", "UniRef:UniRef90_Q9PPU0" ], "nt": "GTGATTATTGATGAACAAGATCAAAATTATTTATTACATAAGAATTATTTACTTCAAATTTTAGATCGTACTGATTATTTAGTAGATTTAAAAGATTGTATTGAAATTATTAAGAATGCTTCAATTAAAAATAAACTTGATGTTTTTGCAAATGAGATTTTAGCAACCCAAATTTCATTAATTAACGCTAAAGATCAATTTAAAGAAATGCATGAAAAATTTTTAGAAATTCTTGCATCACGAACCGAAGATACTATTGAAAATATGGAATTAATTGCTAATCGATATTTTGAGAAATTAAATAAAATAGGCAATAGCGGGATTATTCCTGGAGTTATTAAAACTAAATACGATAATATTGATAAATTCACTAATGGTTATAAACCTGGAGAATTAGTTGTTATTGCCGCTCGTCCTGGAATTGGAAAAACTACATTTTGTTTAAATGTTATGGTTAACAATGTTAATGAAATTATTGAATATAACGAAAATATTCAACCAAATCAAAAAGAAAAGATCATTGTAATGTTTTCGCTTGAAATTACTAAAGAACAAATTTTACAAAAGTTTATCTCAATTAAAACAGGAATTTCTAATCGTGAAGTTATAGAAAATAAATATCGTATAACTAAAGGATATGACACAAGGTCTTTTGCTATGCAAGCAATCAATGATATTAAAAGTTGA", "aa": "MIIDEQDQNYLLHKNYLLQILDRTDYLVDLKDCIEIIKNASIKNKLDVFANEILATQISLINAKDQFKEMHEKFLEILASRTEDTIENMELIANRYFEKLNKIGNSGIIPGVIKTKYDNIDKFTNGYKPGELVVIAARPGIGKTTFCLNVMVNNVNEIIEYNENIQPNQKEKIIVMFSLEITKEQILQKFISIKTGISNREVIENKYRITKGYDTRSFAMQAINDIKS", "aa_hexdigest": "3f46696a3c3eaf2f6b0202cb8ceae0d6", "start_type": "GTG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "both", "pseudogene": { "start": 684688, "stop": 685887, "observations": { "mutation": [ 684993, 685818 ], "stop": [ 684993, 685818 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPU0", "query_cov": 1.0, "subject_cov": 0.4691358024691358, "identity": 0.956, "score": 429.0, "evalue": 6.15e-148, "gene_start": 1, "gene_end": 228, "reference_start": 71, "reference_end": 298, "reference_sequence": "MSTKDLNDAILDLFCLVINNNDFWKDVILRLEAKDFPEKVQQNIFNTIANLNEQKYKISESNILNGLGNYVIVDEQDQNYLLHKNYLVQILERTDYLVDLKDCIEIIKNASIKNKLDLFANEILSTQISLTNAKDQFKEMHEKFLEILASRTEDTIENMELIANRYFEKLNKIGNSGIIPGVIKTKYDNIDKFTNGYKPGELVVIAARPGIGKTTFCLNVMVNNVNEIIEYNQNIQPNQKEKIIVMFSLEITKEQILQKFISIKTGISNREVIENKYRIAKGYDTRSFAMQAINEIKSWPIFVDDRPNISIVDIEAKLYDLKKRYDIALVVLDYLQLVSAGNANKNMTRTQEVGRVSSALKVIAKEINAPVIAIAQLSRKAEERDVSSNANMKNNPLVKTIDNSPKLSDLRESGSIEQDADVVAFLHWDRKQRNAMQNDNQETRMRDDLIEAKFIVEKNRNGSTGETDIIFSKLNSKFIRATTSKE" }, "paralog": false, "identity": 0.9398496240601504, "score": 740.0, "evalue": 6.35e-273, "description": "Internal stop codon at 684993, 685818. Nonsense mutation around 684993, 685818.", "psc": { "uniref90_id": "UniRef90_Q9PPU0", "gene": "DnaB", "product": "DNA 5'-3' helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_Q9PPU0", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPU0", "EC:5.6.2.3", "UniRef:UniRef50_Q9PPU0", "COG:COG0305", "COG:L", "KEGG:K02314" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPU0" ], "product": "DNA 5'-3' helicase" } }, "id": "GPKLONFDEH_1166", "locus": "GCA000006625_01164" }, { "type": "cds", "sequence": "contig_1", "start": 685893, "stop": 686330, "strand": "-", "frame": 2, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_006688495.1", "SO:0001217", "UniParc:UPI0000134210", "UniRef:UniRef100_Q9PPT9", "UniRef:UniRef50_Q9PPT9", "UniRef:UniRef90_Q9PPT9" ], "nt": "ATGAAAGTTATTTTATTAGAAGATATTGCAAGTCTTGGTAAAAAAAATGAAATTATTGATGTTAGTGATGGTTATGCAAAAAATTTTTTAATTCGACAAAAAAAAGCAGTTGCATTAACAAATAAATCACAAGAAGTTTTAAATAAAGATCTTGCAATTTTAGAAGCTCAAGAACAACAAGCAATCTTAGACGCAACTTTACTAAAAGATAAACTAGAGCAAAAGCCTCTACAATTTTTTTTAAAAACAAATAATTTACAAACTTTTGGATCAATTAGTAATAAACAAATTATTGATGAAATTAATAAAGAACAAAAATTTATAACAAAGCATATGATAACAAAACCACATGCATTAGGTATTGGTGAACATATTGTTGAAATTTTATTGCATAAAAAGGTTACTGCTAAACTTCATGTTATTGTTAGCAAAGAATAA", "aa": "MKVILLEDIASLGKKNEIIDVSDGYAKNFLIRQKKAVALTNKSQEVLNKDLAILEAQEQQAILDATLLKDKLEQKPLQFFLKTNNLQTFGSISNKQIIDEINKEQKFITKHMITKPHALGIGEHIVEILLHKKVTAKLHVIVSKE", "aa_hexdigest": "66414708c3bf5a20502ba3adb79c8d62", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000134210", "ncbi_nrp_id": "WP_006688495.1", "uniref100_id": "UniRef100_Q9PPT9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134210", "RefSeq:WP_006688495.1", "UniRef:UniRef100_Q9PPT9" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPT9", "uniref90_id": "UniRef90_Q9PPT9" }, "psc": { "uniref90_id": "UniRef90_Q9PPT9", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_Q9PPT9", "cog_id": "COG0359", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPT9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPT9" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "GPKLONFDEH_1167", "locus": "GCA000006625_01165" }, { "type": "cds", "sequence": "contig_1", "start": 686414, "stop": 686653, "strand": "-", "frame": 3, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181", "KEGG:K02963", "RefSeq:WP_006688517.1", "SO:0001217", "UniParc:UPI0000134DBA", "UniRef:UniRef100_Q9PPT8", "UniRef:UniRef50_Q9PPT8", "UniRef:UniRef90_Q9PPT8" ], "nt": "ATGGCCAAAGTAACTAATAACCGTAATCGTAAACCACGTAAAAAAGTTTGTATTTTATCTGCTAAAGGAATCGAACATGTCGATTACAAAGATGTAGAATTACTACAACGTTTTATTAACAATAATAACAAAATTGCATCACGACGTGTAACAGGAGCAAGTGCTCGTATGCAAAGAAGAATTGCAAATGCAATTAAACGTGCACGTTTCGTAGGATTACTTCCATACGTTAAAGAATAA", "aa": "MAKVTNNRNRKPRKKVCILSAKGIEHVDYKDVELLQRFINNNNKIASRRVTGASARMQRRIANAIKRARFVGLLPYVKE", "aa_hexdigest": "ba659bfab0c8569ca624e2dba836e1fc", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000134DBA", "ncbi_nrp_id": "WP_006688517.1", "uniref100_id": "UniRef100_Q9PPT8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DBA", "RefSeq:WP_006688517.1", "UniRef:UniRef100_Q9PPT8" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPT8", "uniref90_id": "UniRef90_Q9PPT8" }, "psc": { "uniref90_id": "UniRef90_Q9PPT8", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_Q9PPT8", "cog_id": "COG0238", "cog_category": "J", "kegg_orthology_id": "K02963", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPT8" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "GPKLONFDEH_1168", "locus": "GCA000006625_01166" }, { "type": "cds", "sequence": "contig_1", "start": 686689, "stop": 686817, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGAATCATATACAGGTATTAATACTGATGCAGCATTTAGTTCAAACAAACCTCAAACTACCCTTTCATCGACAACATCAAATTTAAATAAAAATAATGATGAAGAAGATGAAATTACAAGTTGA", "aa": "MMESYTGINTDAAFSSNKPQTTLSSTTSNLNKNNDEEDEITS", "aa_hexdigest": "50d2c2f26a4940a45bc3649525f9e719", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4587.783, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_1169", "locus": "GCA000006625_01167" }, { "type": "cds", "sequence": "contig_1", "start": 687016, "stop": 687162, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00436.31" ], "nt": "ATGAATAAAGTAATATTGATTGGTAATTTAGTACGTGATCCAGAAGCTCGTCAAATACCAAGTGGGCGTTTAGTTACTAACTTTACAATCGCTGTAAATGACAATACACCTAATGCTAATGCAAATTTTATACGTTGTGTTGCTTGA", "aa": "MNKVILIGNLVRDPEARQIPSGRLVTNFTIAVNDNTPNANANFIRCVA", "aa_hexdigest": "2c5eb9c90507e308c61ffe649885def2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00436.31", "name": "SSB", "length": 54, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.9075261202521638e-15, "score": 51.83924865722656, "start": 1, "stop": 48 } ], "seq_stats": { "molecular_weight": 5251.998200000002, "isoelectric_point": 9.48924655914307 }, "id": "GPKLONFDEH_1170", "locus": "GCA000006625_01168" }, { "type": "cds", "sequence": "contig_1", "start": 687207, "stop": 687689, "strand": "-", "frame": 2, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904", "KEGG:K02990", "RefSeq:WP_006688782.1", "SO:0001217", "UniParc:UPI00001350CF", "UniRef:UniRef100_Q9PPT6", "UniRef:UniRef50_Q9PPT6", "UniRef:UniRef90_Q9PPT6" ], "nt": "ATGGCGAAATACGAAATTATGTTAGTTGTTCGTGGTGATTTAGATCAAGAACAAGCTAATAAAGTTGCAAATGAATTAAAAGCAACTTTAAAGAATACAGAAGTAAAAGAAAATAATTATGATGGCGTGCAACAATTAGCTTATGAGATTAATAAGTTAAAAACAGCTTATCGTTATGTTTATAATTTTGAAACTACTGATGTTAGCCTAATTAATGAATTTAGACGTTTAGCAATTATTAATAAAAATGTATTGAGACATATTATTATTAATTTAGAAAAAGATTACGGTTATAAAGCAACTGTTAATACTAAAAAAGTACAACGTAATGAAAAACGTGCTGAAGTTTATGCACGTCAAAAAGAAGAAGCAGAACGTCGTGCAGTAGAACGTCAAGCAGCTTATGAAGCAATGAAAGCCGAACGAGAAGCAGCTGGTTTGCCAGTTAAAGAATTTGTTAAAAACACAAATTCAAAACGTTAA", "aa": "MAKYEIMLVVRGDLDQEQANKVANELKATLKNTEVKENNYDGVQQLAYEINKLKTAYRYVYNFETTDVSLINEFRRLAIINKNVLRHIIINLEKDYGYKATVNTKKVQRNEKRAEVYARQKEEAERRAVERQAAYEAMKAEREAAGLPVKEFVKNTNSKR", "aa_hexdigest": "09e7ad96fc14648e81116574cd17fb7d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00001350CF", "ncbi_nrp_id": "WP_006688782.1", "uniref100_id": "UniRef100_Q9PPT6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001350CF", "RefSeq:WP_006688782.1", "UniRef:UniRef100_Q9PPT6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPT6", "uniref90_id": "UniRef90_Q9PPT6" }, "psc": { "uniref90_id": "UniRef90_Q9PPT6", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_Q9PPT6", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPT6" ], "product": "Small ribosomal subunit protein bS6" }, "genes": [ "rpsF" ], "id": "GPKLONFDEH_1171", "locus": "GCA000006625_01169" }, { "type": "cds", "sequence": "contig_1", "start": 687858, "stop": 688367, "strand": "+", "frame": 3, "gene": "argA", "product": "N-acetyltransferase", "db_xrefs": [ "COG:COG1246", "COG:E", "RefSeq:WP_006688484.1", "SO:0001217", "UniParc:UPI00000C1CB9", "UniRef:UniRef100_A0AAC9T1W4", "UniRef:UniRef50_A0AAP9D7L1", "UniRef:UniRef90_A0AAC9T1W4" ], "nt": "ATGGTTTTGATAGAAAAAATCAATGAACAAGATATTAATACCGTCGTAGCATATGTTGTTGCTTTCCGTAAAAAACTTTATCCCAAAGTTGATCATAATAACTTACCTAAGGAACTAGTTAATTTTAAACAAACTTACATTATTAATCCTTTAGGAATATGGTTTAGTGTTTTTTTAGAAGATACACATGAATTAGTTGGAACAATTTCATGTCATGAATACGATTATAGATATAATAATCTATTCTATCTAGATAGAAGTACTAAAACAAGTGAGGTTGGTAAATTATATATTGATCCTTTGATTCGACGTCAAGGAATTGCAACTCGTTTGTTTAACGCATTAAAAGACCAAGCTAAAAAACAAGGAATTAAATGTTTTTATTTACACACACACCATCACTTGCCAGGCGCTAGAGAATTTTGGTTAAAAATGGGATTTAAAATTCAAAAAGAAATGACTTTAGAAGATGATGGTCAAGACATTATTCATATGACTTTAGAATTATAA", "aa": "MVLIEKINEQDINTVVAYVVAFRKKLYPKVDHNNLPKELVNFKQTYIINPLGIWFSVFLEDTHELVGTISCHEYDYRYNNLFYLDRSTKTSEVGKLYIDPLIRRQGIATRLFNALKDQAKKQGIKCFYLHTHHHLPGAREFWLKMGFKIQKEMTLEDDGQDIIHMTLEL", "aa_hexdigest": "46a633c9b28f01cf8ae83d16da6620c4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000C1CB9", "ncbi_nrp_id": "WP_006688484.1", "uniref100_id": "UniRef100_A0AAC9T1W4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CB9", "RefSeq:WP_006688484.1", "UniRef:UniRef100_A0AAC9T1W4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T1W4", "uniref90_id": "UniRef90_A0AAC9T1W4" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T1W4", "gene": "ArgA", "product": "N-acetyltransferase", "uniref50_id": "UniRef50_A0AAP9D7L1", "cog_id": "COG1246", "cog_category": "E" }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D7L1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D7L1" ], "product": "GNAT family N-acetyltransferase" }, "genes": [ "argA" ], "id": "GPKLONFDEH_1172", "locus": "GCA000006625_01170" }, { "type": "tmRNA", "sequence": "contig_1", "start": 688472, "stop": 688884, "strand": "-", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 688555, "stop": 688641, "aa": "AENKKSSEVELNPAFMASATNANYAFAY", "nt": "AAATTTAAAATTCTTATTTGTTGTTTAAATTTAAATAGATTTAACAAATAGTTAGTTAATTTTAAATTTGTTTTATTAGTTATTAAC" }, "nt": "GGGGATGTCACGGTTTCGACGTGACACATTAATTTTTAATTGCAGTGGGGTTAGCCCCTTATCGCTTTCGAGGCATTTTAAATGCAGAAAATAAAAAATCTTCTGAAGTAGAATTAAACCCAGCGTTTATGGCTTCAGCTACTAATGCAAACTACGCTTTTGCGTACTAATTAGTTATTAGTAGAAACGTTCATTAACATAATTACTATTGGTTGGTTTTTGGGCTTATTTTACAATAGTTTTAAATTTAAAATTCTTATTTGTTGTTTAAATTTAAATAGATTTAACAAATAGTTAGTTAATTTTAAATTTGTTTTATTAGTTATTAACTACACTATTTTTAATAAAACTAAACTGTAGATATTATTAATTATGTGTTGCGGAAAGGGGTTCGACTCCCCTCATCTCCACCA", "id": "GPKLONFDEH_31", "locus": "GCA000006625_01171" }, { "type": "cds", "sequence": "contig_1", "start": 689319, "stop": 689507, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGAATTAATGAAATAATTAAAGCTTTTTTAACTTTCGTTTTATTTTCATTAAAATCATTTAAATACTTAATTAAAATGCGAATATTAACAACTAAGCTAATTAAATATGTTAATGTTCATAAAACTAAAAATAGGCCAAAAAAACCAATTGATAAACGAATTTGTTCTTTTTGTGATAAATAA", "aa": "MKGINEIIKAFLTFVLFSLKSFKYLIKMRILTTKLIKYVNVHKTKNRPKKPIDKRICSFCDK", "aa_hexdigest": "0e633f300074a2fa21ee93e91146b88b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7392.0479000000005, "isoelectric_point": 10.305674171447752 }, "id": "GPKLONFDEH_1173", "locus": "GCA000006625_01172" }, { "type": "cds", "sequence": "contig_1", "start": 689441, "stop": 689590, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTCATAATTTATTTGATGCATATACCCTAAATCATGAAAAAGAATTAATTAACATGTTAAATCCTAAAGATTTAGAAGTTTATTTATCACAAAAAGAACAAATTCGTTTATCAATTGGTTTTTTTGGCCTATTTTTAGTTTTATGA", "aa": "MRHNLFDAYTLNHEKELINMLNPKDLEVYLSQKEQIRLSIGFFGLFLVL", "aa_hexdigest": "2559ecf46ff3a56e94ebf54f89a1b403", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5852.780399999998, "isoelectric_point": 6.012735557556151 }, "id": "GPKLONFDEH_1174", "locus": "GCA000006625_01173" }, { "type": "cds", "sequence": "contig_1", "start": 689710, "stop": 689811, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAACTTTACATATTATTTACAATTTGCTTATGATTATCGAAGACCTCCACGTCCTGGTTTACCAACATCACCACAAGCAGAAGGGGATAATTAA", "aa": "MKNNFTYYLQFAYDYRRPPRPGLPTSPQAEGDN", "aa_hexdigest": "d5487bdb482ceddcf82e6d31143b4651", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3908.270800000002, "isoelectric_point": 8.138182640075684 }, "id": "GPKLONFDEH_1175", "locus": "GCA000006625_01174" }, { "type": "cds", "sequence": "contig_1", "start": 689932, "stop": 690390, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAACGTGAATTTTATTTATCAGAAGAGTTCTTAAATAAACACGATCCAAAACGTGAAAATAAACCGTTTTTACTAGTATTGCAAAATAATGTAACAGGTCAATTTACAACGCTACAAATTCAACCAGTTATCGATAAAAAAACAAATCAATATGTTCAAGCTACAAAAGAAGATTATGCAAAATATTATAATAAAGAAAATATTGATGAAATTAATTTCAAAAAACGATTTAAATACAAATTTTCTATTAAAAAATTACCAATTGGATCATATCAAGTAATCAACTTATCTTACATAAATGATTTAAAACCATTATTAAGTAAGTACTTAAGAAATGAGAATAATGAAATATCTGATAAAGTTAATAATGAAATTTATATGACAAAATTAGAAACAGTTGTTGATCAACGTAAAAGAATTATTGATTTCTTTTCAGGGAATCGTCCAGGTTGA", "aa": "MQEREFYLSEEFLNKHDPKRENKPFLLVLQNNVTGQFTTLQIQPVIDKKTNQYVQATKEDYAKYYNKENIDEINFKKRFKYKFSIKKLPIGSYQVINLSYINDLKPLLSKYLRNENNEISDKVNNEIYMTKLETVVDQRKRIIDFFSGNRPG", "aa_hexdigest": "03c38d2faca79dde99ae34009e1c2d55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18243.596599999993, "isoelectric_point": 9.31782512664795 }, "id": "GPKLONFDEH_1176", "locus": "GCA000006625_01175" }, { "type": "cds", "sequence": "contig_1", "start": 690430, "stop": 690702, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACGTTTACATATTAGTGAAGAAGACGCTGATATTATTCGTGAATTGGTAACGCCAACAAATATTAAAACAAGTGAAGTTTATAATCCACAACACCTAACAAATAGAGCTGATTTACGTAAATTAAAATTAAAAGATAAAGCCATTCCAATGTTTGATGTTAATTATTCTGAATATGACGCTAAGATCATTCATGATTTAGAACACCAAATTAATAAATTAAATCAAAAAATTAAAAATGAGCCTACAAGTAGTTCGAAATCAACGTGA", "aa": "MKRLHISEEDADIIRELVTPTNIKTSEVYNPQHLTNRADLRKLKLKDKAIPMFDVNYSEYDAKIIHDLEHQINKLNQKIKNEPTSSSKST", "aa_hexdigest": "052f6527268b7bf283afc7feb7a29544", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10502.820699999991, "isoelectric_point": 7.987520027160644 }, "id": "GPKLONFDEH_1177", "locus": "GCA000006625_01176" }, { "type": "cds", "sequence": "contig_1", "start": 690757, "stop": 690921, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACATTTGCAAATAGTGGTAAATTTGATATTTCTTATCAAAACTTTGATAAATATGCTGGTAGTACTTATGACTCAGCATTAGGATTATTTTTAACACCTGATGGAATTGATTTAAAAAATAATAAAAGCTCAGGTTATGAATTAAATCCAACAGGATCTTGA", "aa": "MTFANSGKFDISYQNFDKYAGSTYDSALGLFLTPDGIDLKNNKSSGYELNPTGS", "aa_hexdigest": "2047728d194933a83f1a971e225e7797", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5862.3135, "isoelectric_point": 4.460405921936036 }, "id": "GPKLONFDEH_1178", "locus": "GCA000006625_01177" }, { "type": "cds", "sequence": "contig_1", "start": 691369, "stop": 691566, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGATTTAATGAAAAAACAAGCAGGGGCTGGAACGGTTGCAATGCAATTTAATTTAGATCGTCAATCACGTTATGATAGTTATCAAGAATATTTAAAAAATCCTAATAAGGACATTCCAATCCTAGATCCTGATTTACGACGTGCAATTGCTTTTGCTATTAACAGAGAAAACATATTAAAATTATCTGGTTGA", "aa": "MRDLMKKQAGAGTVAMQFNLDRQSRYDSYQEYLKNPNKDIPILDPDLRRAIAFAINRENILKLSG", "aa_hexdigest": "8fb3fa5523bd3238f0d78d94e14168fd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7513.5533, "isoelectric_point": 9.518063926696776 }, "id": "GPKLONFDEH_1179", "locus": "GCA000006625_01178" }, { "type": "cds", "sequence": "contig_1", "start": 691789, "stop": 692028, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAAGTGATGATTTACAAACTTTAGCTAATTCAAAAGATTTATTTAAATTTAAATCATTACCAACTTCACGTTTTGATTTACTAATGGAATTTGAGACTTATGCTCCAAAACCTTCATTTAGTGTTTTATTGACTGATATTTTAGGAAACCAAGCTTTACTACCAGTTAATCGTCGTTTTATTGAAACACATGGTGGAATTGAAAAATTCGGTTCTACTCTTGAAAATTTTGTTTGA", "aa": "MASDDLQTLANSKDLFKFKSLPTSRFDLLMEFETYAPKPSFSVLLTDILGNQALLPVNRRFIETHGGIEKFGSTLENFV", "aa_hexdigest": "e1652d3ca7009132296449942dc1399b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8913.103699999996, "isoelectric_point": 5.180385398864746 }, "id": "GPKLONFDEH_1180", "locus": "GCA000006625_01179" }, { "type": "cds", "sequence": "contig_1", "start": 692647, "stop": 693105, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAAATAAGAAAATTATATTTTTATCTATTGGAATTGTTGGTTTAATTCCCGTTGCTGCGATTATTGCTTCTTGTGCTAGAACTAAGACCACTAAAGAACAAAATAAAATTAGTCCATATTTAGAAGATGGTAGTAATTTTGGTTTGGCAATGGATGCAATCAACACATTGAATTATGTAAAATTTGCATCGCTTCGTAAAATTGTATTTTCACTAGTAGATGGTTTAATAAAAGAAGCGCCATCAACAAATTCAACAATCGGTAACTTATTAGGAATATCAAAGTTGTTTTTAAATCAATATAAAAATCCAGTTGATGCAGGTTTTAATAATATGACTGATGCACAATTAGAAGGTATGAGAATATCACCATCATATGTTGATTTATCATCTTTTGGAATTAATTCTGGAGCGTTAGCACCAACAGTTAGTGAAGATGATCCAACATGA", "aa": "MKKINKKIIFLSIGIVGLIPVAAIIASCARTKTTKEQNKISPYLEDGSNFGLAMDAINTLNYVKFASLRKIVFSLVDGLIKEAPSTNSTIGNLLGISKLFLNQYKNPVDAGFNNMTDAQLEGMRISPSYVDLSSFGINSGALAPTVSEDDPT", "aa_hexdigest": "2cc4f61222aa0e45d6927523f05bdf37", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16332.697999999978, "isoelectric_point": 8.663599967956543 }, "id": "GPKLONFDEH_1181", "locus": "GCA000006625_01180" }, { "type": "cds", "sequence": "contig_1", "start": 693124, "stop": 693318, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAAAGATCTAAAAGAAGTTATGGATTTAAGTGATCCAAAAAACCCAAAAAGTTTAATGACTTTAGGTTCACGCAAATATGTTGCTGAAAACGGTAAATCATATACTGTTTCACCAGTTTATGCAAGAAACGAAAATACAAAAGATAAAAATCGTACAATTATTAGAATTTCAAACCAATTCTCAGTTTAG", "aa": "MDKDLKEVMDLSDPKNPKSLMTLGSRKYVAENGKSYTVSPVYARNENTKDKNRTIIRISNQFSV", "aa_hexdigest": "b9bf11321dccc8017dad89ff733e4889", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7323.260600000004, "isoelectric_point": 9.571637153625485 }, "id": "GPKLONFDEH_1182", "locus": "GCA000006625_01181" }, { "type": "cds", "sequence": "contig_1", "start": 693442, "stop": 693597, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGGTTTCTTTAAAAAAGAATATAAGGATAAAGTTAAAAAAATTGCAATAAAAAATAAAAATAATGGCAAAATAGAATATATCGATGTTCACACAGATAATACAAATAATTTATTTTATCTAAAACGTCAATCAGACATTAATTCTAAATGA", "aa": "MYGFFKKEYKDKVKKIAIKNKNNGKIEYIDVHTDNTNNLFYLKRQSDINSK", "aa_hexdigest": "c6df0d89cd1df0bc17f2a1e3a8c004eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6143.978900000002, "isoelectric_point": 9.698639869689941 }, "id": "GPKLONFDEH_1183", "locus": "GCA000006625_01182" }, { "type": "cds", "sequence": "contig_1", "start": 694648, "stop": 694773, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGATGGCAAACTAGGTTTATATGGATTTAGAAGTTTTGGTAATAAATATGAAAGACCAGGCTCACCATATGGAGAGCCACTTGATACATATAATTTTGCTGGTAATCCACAGAATGTTTGA", "aa": "MFDGKLGLYGFRSFGNKYERPGSPYGEPLDTYNFAGNPQNV", "aa_hexdigest": "0d71b8cdae9bd9ed575a06bda2ff5ac3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4605.017800000003, "isoelectric_point": 5.970674705505371 }, "id": "GPKLONFDEH_1184", "locus": "GCA000006625_01183" }, { "type": "cds", "sequence": "contig_1", "start": 694984, "stop": 695322, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATTTAAATGAATTTGGAACTATTAAGTTGATTGTTGATAGAGGTGATGGAAAAGATAATAAACATTTAAAAAAGCTTGACGAATTAACAGAAAAAGAAATTTACCCATTAATGATTTTAACGCCTGCTGAAGCACAAGCAATTAATTTTGATTATCTAAAATCAAATAATGGATCATTCACTCCTCTTACACTTGAAAATAATGGATCAAAATTCTACACAATTAAATTTAAAGATATTCCTTCTTTAATTGAATTTATGTCTGTGGATCCAGCTAAATATACAATTGTGGAAAATGCTTTAAGCTCAAGTCATGAAAATGTTCGTAAATGA", "aa": "MINLNEFGTIKLIVDRGDGKDNKHLKKLDELTEKEIYPLMILTPAEAQAINFDYLKSNNGSFTPLTLENNGSKFYTIKFKDIPSLIEFMSVDPAKYTIVENALSSSHENVRK", "aa_hexdigest": "4a54122dd0ba3d2b44e4b1bb16b3970e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12725.408399999984, "isoelectric_point": 5.662380027770996 }, "id": "GPKLONFDEH_1185", "locus": "GCA000006625_01184" }, { "type": "cds", "sequence": "contig_1", "start": 695641, "stop": 695826, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATACCCTGATTTTAACATTAGCAAATCTTTAAAATTAGATGAAAAAGCGAATGCATCATCTGTTCCATATTATAAAAAAGTTCTTTCGCAAATTATTGGCGAAGATATTACAAATAAAACCTTTGACCAATTTAAATATGCTTTATTAACTTTACAATACAATGCATCAAAAATTTGA", "aa": "MFKYPDFNISKSLKLDEKANASSVPYYKKVLSQIIGEDITNKTFDQFKYALLTLQYNASKI", "aa_hexdigest": "61acc190ace28e118362c9259a1a8520", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7071.0632000000005, "isoelectric_point": 9.223378944396973 }, "id": "GPKLONFDEH_1186", "locus": "GCA000006625_01185" }, { "type": "cds", "sequence": "contig_1", "start": 695938, "stop": 696387, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCATTTGATCATGAATCTGCAACTGTTAACCCATCATTTGGCCCTGACATTAAAGATCCAAGTCGTTTAAAAGCTGAATATTATTTTTATAATGATCAAAATAGTCAAGAACAAAAAAATGATAATTTTAAATTTGGTAGTGTTATTGAAAAACCAGGACTTTCAAAATATGATGGTATTTTAAAAGATGGTATGGGAACACCAATTAAATTTAGTAAAGATGGTAGAGCAATTGTTTATAAACTTCACAAGAAAAAACATCCATACAAAAAAGATGATATTGAAATTTTAATTAAAACCAAAAACAATACACCAGTAATTGATTTATCAACTTGTTTAAAATCAGATGGGACAATTAACACAAGAAAACTTAATGAAAAAGTGAGAGAGATACAAGATTCAATTAATAGCCTAATCGTTAAAAATTACTATAATGGTGGTTGA", "aa": "MNSFDHESATVNPSFGPDIKDPSRLKAEYYFYNDQNSQEQKNDNFKFGSVIEKPGLSKYDGILKDGMGTPIKFSKDGRAIVYKLHKKKHPYKKDDIEILIKTKNNTPVIDLSTCLKSDGTINTRKLNEKVREIQDSINSLIVKNYYNGG", "aa_hexdigest": "780b1eaaad5975263f59b3373ae32d8b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16996.00359999999, "isoelectric_point": 9.127772331237793 }, "id": "GPKLONFDEH_1187", "locus": "GCA000006625_01186" }, { "type": "cds", "sequence": "contig_1", "start": 696535, "stop": 698295, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGACTTTGAAAGAATTTAAATTAAGTCGTGGAATTCAACAAAATGGTAACGCAATTACACTAGGGTTACATAGTGGTACAAGAGAAAGAACAGTAATTGAATTCTTCCCAGATGCTTTTTTTGGTTCATTACCAATTTATAATATTAATGCTGGCCCCGGCAATGCTTATGATTCATTAGCACGACAAGTTAATAAAGATGCTATGACATTAGAAGAATTAGAAGAATATCATCAAAAAATTCCTTTAATGATTTCAGCATATAATAGCCATAGTTTTGGTGGTGTTAAATTAATTGATGTCCTAAAAAACGCAAAAAATACTTATTTATTTAATGCTGATAAGTACTTTGATTTAACAAAAGATCCACTAAATTTTCCTAATTTAAAAAATCTTAAAGCAAGTGATCGAACATTAAATTATCTTTTTTATGCTCAAAACGAACAACAAGCAAAACAAAAAGTACAAGCATATTTAAACGAATTACAATTTGTTAAAGGGTTAATTCCAAAAGATAAAAAATTTAATCCTAAAATCGACGTTAAAATGGATGAAATTAATAAAATTAAAATTAATGAATTTTTGGAATTTAATGCTAGTGCTTCATCTCCTTTTGGTGGTTCGCTTGATCTTGAATCAATTGGTAAATCAAGTGGTAATCAAAATTATTTACTAATTGATGCAACCATTGGTGGCCAACAACAAAAAATTAAATACTACATGTTTAAGGATGAATTCACTACTAAATCAAGTTCAAATGATGAATTTATTCAAAATGATAATAGTATTTTAGCATCAAGTTTACGTGAAATAATTACTAAAAATTTTAATGATTATATTGATCAAAAACAAAATTTTAAAAATGTTTATGATGTTGAATCTACATTTAAAAATAAACAATTTTATATTTATGATATTGATGGTAATGGTAAAAATCCCCATTTTTATAAATCAAAAGAATCATTATTAGAATCTGAATTAGTTAACTATGATGAGAAAAAAGTTTCATTGGTAACTATTACTAAAACAAAAGTCGAAGGTTTAAATAAACTAATTTTAACAACAAAAGATGATAAAGATTTAATTTTAGAATCACCTAAAAATAATCCAAAAGGTTATAATTATGATTCAAGTTTTGATGATGCTTTCAACACTCTTAAAGTTGCGGCTAATTATTTTGATGTTTTTACACCTCGTTTGATTAAACAAGGAAGTAAATTTGTTAACGGCAAAATTATTAAAACTTATACTATTTATGTTGATGCATATAGCGGTTTATTAGATAAGGTTTTAAATAAAAATCGTCATTTATTACAAAAAATTAATGGTATTCATACAGAAGTTGTTCAAGATAAATATGGTAAAAATACATATAAAGTTGTTAATGGTGAATACGAAGGTATTTATGTAACTGATCGAATTCCTTATTTATCATTAGTTGCTCAATCTGATCCTGCATTTAAAACAACAGGTATTAACTATTTAAAATATGTAAGTACACACGAATATGGACATCACCAAACATTACAAGACATGAAAGATATTAGTGATAGTAATGATTCAGTTATTGGTGGAGGAATTGATTCTAGATCGGGAGTTAGTGATGAATCATATGTAAATGGTAAGGCATTACAAGATTATTTAAATGCACGTTCTAGCGGAATTACCTTTAGAAAAACTGATGTTGATTATAATCCAACAAAAGATGGGTCATTCTTTAATTTTTCATTAAATAACGATCCTAAAAATCCAGTTTGA", "aa": "MLTLKEFKLSRGIQQNGNAITLGLHSGTRERTVIEFFPDAFFGSLPIYNINAGPGNAYDSLARQVNKDAMTLEELEEYHQKIPLMISAYNSHSFGGVKLIDVLKNAKNTYLFNADKYFDLTKDPLNFPNLKNLKASDRTLNYLFYAQNEQQAKQKVQAYLNELQFVKGLIPKDKKFNPKIDVKMDEINKIKINEFLEFNASASSPFGGSLDLESIGKSSGNQNYLLIDATIGGQQQKIKYYMFKDEFTTKSSSNDEFIQNDNSILASSLREIITKNFNDYIDQKQNFKNVYDVESTFKNKQFYIYDIDGNGKNPHFYKSKESLLESELVNYDEKKVSLVTITKTKVEGLNKLILTTKDDKDLILESPKNNPKGYNYDSSFDDAFNTLKVAANYFDVFTPRLIKQGSKFVNGKIIKTYTIYVDAYSGLLDKVLNKNRHLLQKINGIHTEVVQDKYGKNTYKVVNGEYEGIYVTDRIPYLSLVAQSDPAFKTTGINYLKYVSTHEYGHHQTLQDMKDISDSNDSVIGGGIDSRSGVSDESYVNGKALQDYLNARSSGITFRKTDVDYNPTKDGSFFNFSLNNDPKNPV", "aa_hexdigest": "b1633bb5692eda73933bec1fc27f97f7", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 66575.97690000007, "isoelectric_point": 7.124619865417481 }, "id": "GPKLONFDEH_1188", "locus": "GCA000006625_01187" }, { "type": "cds", "sequence": "contig_1", "start": 698326, "stop": 698775, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGGTTAAAATTATCAGCTAAAATTGCAATTGCTTTTGCATCAGTTGGTGCATTTGCTGGTGCTGTTGTTGGTTTTATGAAATTATATGCTGTTTCTACTGGTGGACTTGGACGTCAAACATTACAAGTTAACAGTAGTTTTGATACGACAATTCCTAATCGTCAAGTAGATCGTGCTTTATTAATGGATCAATTTGGCAAACCAGTTGCTGATTATGATTTTAAAGCAAAAAAAGGTAATAATACACAAGTAACATTATTAATTGATTTTGGAAAATATAAAAAAGGTCAGAAAATTTCTTATCTTGAATTTTTAGATAATTTTGTTAATTTAAATAACAACAACTTACCAAATTTAAGATTAGAAGTGGGCCCAATTGTTTTCAATAACAATTATATCAATAGTATTTCTCCAGACGAATTCATTGAGTTTACTAATTGA", "aa": "MKKRLKLSAKIAIAFASVGAFAGAVVGFMKLYAVSTGGLGRQTLQVNSSFDTTIPNRQVDRALLMDQFGKPVADYDFKAKKGNNTQVTLLIDFGKYKKGQKISYLEFLDNFVNLNNNNLPNLRLEVGPIVFNNNYINSISPDEFIEFTN", "aa_hexdigest": "0abc0760f969275a117cf9f24997af43", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16577.86159999999, "isoelectric_point": 9.583048057556148 }, "id": "GPKLONFDEH_1189", "locus": "GCA000006625_01188" }, { "type": "cds", "sequence": "contig_1", "start": 698869, "stop": 699153, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATATTTTTAAAGAATTAAAAACTAAATATGATTTAACAATTTTATTTATTGCTCATGATCTACGAATGGTTGAATATATTTCAGATCGAATTGCAGTTATGAATAAGGGTGTGTTGTTAGAAATTGGTCCAACAAAAGAAATTGTTAATAATTTCTTACATCCATACACACGTTCTCTAATGGAAGCTGTACCTTCAATTGATTCAGAAAAAAAATCACTATTAGGTTATGTATATGATGCTAACATGCATGAATATACACCAACAAAACAACCAAAATGA", "aa": "MNIFKELKTKYDLTILFIAHDLRMVEYISDRIAVMNKGVLLEIGPTKEIVNNFLHPYTRSLMEAVPSIDSEKKSLLGYVYDANMHEYTPTKQPK", "aa_hexdigest": "88c80cdfd5bf80237bf1d442be897424", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10920.632999999993, "isoelectric_point": 6.826954078674317 }, "id": "GPKLONFDEH_1190", "locus": "GCA000006625_01189" }, { "type": "cds", "sequence": "contig_1", "start": 699370, "stop": 700113, "strand": "-", "frame": 1, "gene": "dppD", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG0444", "COG:EP", "SO:0001217", "UniRef:UniRef50_A0A449AZS4", "UniRef:UniRef90_Q9PPT1" ], "nt": "ATGAGTGATACAAACCAGAAAAAAGCCTTTTTTAATCACAAAAAACGTTTTTGTTTACCAGACAAAATTACTATTCGCCCAGTTACTGAAGGTTTAATAGAACGATTAAATGCAAATCCTGAAAAAAAGCGTTTAGTACAAGTACGTCACTTAGATGTTACTTATGGTCATGGATTTAAAAAATTTAGAGCGGTTAAAGATATTTCATTTAATATTTATCAAGGTGAAGTTTTAGGATTAGTTGGTGAATCAGGATCTGGTAAATCAACAACCGGATCTGCGTTAATTGGTTTAGCGCGTCATAGTTTTGGGGATATTATTATTGATGGTATTAAATTGCCTAAACAAGGTGAGAAAGTTACTAAAGAACTAACTGATTTTATGGTTAATAATGTGCAGATGATTTTTCAAGATCCATCGAATTCACTTAATCCTTATGTAAATATTGAAACAGTTGTTTCAGAAGGATTAAATAATATTAAAGATTTGAAAAAATCTTTCTTACGTTTAATTTATAAAGATTATTTACGAGGATTAAAAAAAGAACTTAAAGCAAAAAATTTTTTAAATAATGAACAAGTACAAATTTTTTTAGAAAAAGCACAAGATTTAAAGTTTGATTATTTAAATGAAACTGCTCAACAAGAATTAATCCAATTATTCAATGAATTAGAATTAAATCATTTATCATTAGATAATAATGATGAAAAATTACAAAAAGCTTTTAATGATATTAATACTTGA", "aa": "MSDTNQKKAFFNHKKRFCLPDKITIRPVTEGLIERLNANPEKKRLVQVRHLDVTYGHGFKKFRAVKDISFNIYQGEVLGLVGESGSGKSTTGSALIGLARHSFGDIIIDGIKLPKQGEKVTKELTDFMVNNVQMIFQDPSNSLNPYVNIETVVSEGLNNIKDLKKSFLRLIYKDYLRGLKKELKAKNFLNNEQVQIFLEKAQDLKFDYLNETAQQELIQLFNELELNHLSLDNNDEKLQKAFNDINT", "aa_hexdigest": "832cc5ac9099df727e6c83c49dc7894e", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dppD" ], "truncated": "5-prime", "pseudogene": { "start": 699067, "stop": 700113, "observations": { "mutation": [ 699372 ], "stop": [ 699372 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPT1", "query_cov": 1.0, "subject_cov": 0.5626423690205011, "identity": 0.951, "score": 458.0, "evalue": 1.58e-159, "gene_start": 1, "gene_end": 247, "reference_start": 1, "reference_end": 247, "reference_sequence": "MSDTNQKKAFFNHKKRFCLPDKITIRPVTEGLLERLNANPEKKRLVEVRHLDVTYGHGFKKFRAVKDISFNIYQGEVLGLVGESGSGKSTTGSALIGLARHSFGDIIIDGVKLPKQGEKVTKELTDFMVNNVQMIFQDPSNSLNPYVNIETVVSEGLNNIKDLKKSFLRLIYKDYLRGLKKELKAKNLANNEQVQTFLEKAQDLKFDYLNETNQQELIQLFNELELANLSLDNNNKKLEKAFNDINTWKSKTRTDITRHLILDVLKSVGLDETVLRRYPLEFSGGQQQRIGISRAVALRPKLLIADEPISALDVSIQAQVVNIFKELKAKYDLTILFIAHDLRMVEYISDRIAVMNKGVLLEIGPTKEIVKNFLHPYTRSLMEAVPSIDSDKKSLLGYVYDANMHGYTHEKQPKWIHLGNDHYILGTEEEVADWKAGKY" }, "paralog": false, "identity": 0.9597701149425287, "score": 654.0, "evalue": 2.95e-239, "description": "Internal stop codon at 699372. Nonsense mutation around 699372.", "psc": { "uniref90_id": "UniRef90_Q9PPT1", "gene": "DppD", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_A0A449AZS4", "cog_id": "COG0444", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPT1", "UniRef:UniRef50_A0A449AZS4", "COG:COG0444", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AZS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AZS4" ], "product": "Phosphate ABC transporter ATP-binding protein" } }, "id": "GPKLONFDEH_1191", "locus": "GCA000006625_01190" }, { "type": "cds", "sequence": "contig_1", "start": 700167, "stop": 700406, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATGCCTGACGTTAATAAAGGTGATCGTTTAGCAACAATTAAAGGTTCTGTTCCATCACGTTTAAATGACATAGTTGGTGATGCATTTGCTATTCGTAATGATTATGCATTAACTCGTGATTTTGAACATGAACCTGAAATGTATCATGTAAGTGAAACTCATCGTGTTAAATCAGCATTATTAGATGAACGTGCTGAAAAGTATACACCTCCACAAATAATTTTAGATAAATGA", "aa": "MSMPDVNKGDRLATIKGSVPSRLNDIVGDAFAIRNDYALTRDFEHEPEMYHVSETHRVKSALLDERAEKYTPPQIILDK", "aa_hexdigest": "00319eb634895b44977cfd238923625a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9014.072299999993, "isoelectric_point": 5.499706840515136 }, "id": "GPKLONFDEH_1192", "locus": "GCA000006625_01191" }, { "type": "cds", "sequence": "contig_1", "start": 700440, "stop": 700877, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13304.12", "PFAM:PF00005.33" ], "nt": "ATGTCAACATTAAATCCAACCATGAAGATTGGTAAGCAAATTATGGAAGGGATGTTAATTAATGGAATTGTTAAGACAAGAAAAGAAGCCTATAATAAATCAATTGAATATTTAAAATTAACAAAAATTAATAATCCTGAAGAAATTATGGAATTATACCCACACGAATTATCTGGTGGAATGATTCAAAGAGTTGTAATTGCCTCAATTGTTTCATTACATCCAAAAATTTTAGTTTTAGACGAACCAACAACAGCGCTTGATCCAACAGTGCAAGCTTTAGTGTTAGATGTTATTCGTGAGTTACAAGAGAAATTTAAAATGTGTATTATTTTTATTACTCACGATCTTGGAGTGGTTGCTTCAATTGCTAATTATATTTCTATTATGTATGCTGGACAAATTATTGAAGAAGGTCAGCGTGATGAAATTTTATGA", "aa": "MSTLNPTMKIGKQIMEGMLINGIVKTRKEAYNKSIEYLKLTKINNPEEIMELYPHELSGGMIQRVVIASIVSLHPKILVLDEPTTALDPTVQALVLDVIRELQEKFKMCIIFITHDLGVVASIANYISIMYAGQIIEEGQRDEIL", "aa_hexdigest": "6c4249f25e5fe1abb950fd38a5048a9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13304.12", "name": "AAA_21", "length": 73, "aa_cov": 0.4827586206896552, "hmm_cov": 0.9863013698630136, "evalue": 9.752494380181102e-09, "score": 31.23350715637207, "start": 51, "stop": 120 }, { "id": "PF00005.33", "name": "ABC_tran", "length": 83, "aa_cov": 0.5655172413793104, "hmm_cov": 0.8192771084337349, "evalue": 2.7987154880351647e-11, "score": 39.77769470214844, "start": 4, "stop": 85 } ], "seq_stats": { "molecular_weight": 16326.136099999982, "isoelectric_point": 5.375229454040528 }, "id": "GPKLONFDEH_1193", "locus": "GCA000006625_01192" }, { "type": "cds", "sequence": "contig_1", "start": 700929, "stop": 701654, "strand": "-", "frame": 2, "gene": "dppD", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG0444", "COG:EP", "SO:0001217", "UniRef:UniRef50_A0A084F1H7", "UniRef:UniRef90_Q9PPT0" ], "nt": "ATGAAACAAAAAAACAACCGATATACTTATATTAACTATCTTCGTAGTCAACGCGCTTTTATTCAAACATTAAAACCACAAATTTTAAAATTAACTTCAAATAATGTTTGTTTAAATGAACAAGAGGTGGATTTAGAACCACAATTAAATAATTTAGAAGACATTAATATACAATTAACTAATGAAAAAGTTAATATTCAAGATATTGTTACTTTAGAAGAAGAAATTAATGTTAAAGATAATAATCAAAATCAAAATTTAAATGAAGAAATCTTAGCAACAACATTAATTAATGAACAGGCTAGTGGTATTAATGAAATTAGTGAACAAAAAGAAACTACAATAGCTAATAAAAAACATAAAAAACAAACAATGGCTTTTAAAGCTGCTAATTCACGAAAAAACTTTGATTTTGAAAAATTTGAAAAAAATGTTAAATATAAAAGTTTTAAAGATGGTACAGTTAAAAAAATTGCAGCTGAAATCGAAAATGTACGTTTAACTTTTACAAACCCATCAAAACCCAATGATCGTGCTTTAGTTTTACGAAATACATCTGTTCAATTTTATGAAGGTGAAGTCCATGCAATTATTGGTGAATCAGGATCTGGTAAATCAGTAATTACTTCTCTTTTGTATGGTTTGGCTGGAAAAAATGCCAATGTTGAAGAAGGTAAAATTTTATTATATAACAATGAAGTTCAAGATTTTAGCTTCAAAGATTGA", "aa": "MKQKNNRYTYINYLRSQRAFIQTLKPQILKLTSNNVCLNEQEVDLEPQLNNLEDINIQLTNEKVNIQDIVTLEEEINVKDNNQNQNLNEEILATTLINEQASGINEISEQKETTIANKKHKKQTMAFKAANSRKNFDFEKFEKNVKYKSFKDGTVKKIAAEIENVRLTFTNPSKPNDRALVLRNTSVQFYEGEVHAIIGESGSGKSVITSLLYGLAGKNANVEEGKILLYNNEVQDFSFKD", "aa_hexdigest": "285d366d0cbdae629983f10e614796ee", "start_type": "ATG", "rbs_motif": null, "genes": [ "dppD" ], "truncated": "5-prime", "pseudogene": { "start": 700626, "stop": 701654, "observations": { "mutation": [ 700931 ], "stop": [ 700931 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPT0", "query_cov": 1.012448132780083, "subject_cov": 0.4793713163064833, "identity": 0.98, "score": 352.0, "evalue": 8.37e-117, "gene_start": 1, "gene_end": 241, "reference_start": 1, "reference_end": 244, "reference_sequence": "MKQKNNRYTYINYLRSQRAFIQTLKPQILKLTSNNVCLNEQEVDLEPQLNNLEDINIQLTNEKVNIQDIVTLEEEINVKDNNQNQNLNEEILATTLINEQASGINEISEQKETTIANKKHKKHKKQTMAFKAANSRKNFDFEKFEKNVKYKSFKDGTVKKIAAEIENVRLTFTNPSKPNDRALVLRNTSVQFYEGEVHAIIGESGSGKSVITSLLYGLAGKNANVEEGKILLYNNEVQDFSFKDWEKSRYLGKVISAVFQNPMSTLNPTMKIGKQIMEGMLINGIVKTRKEAYNKSIEYLKLTKINNPEEIMELYPHELSGGMIQRVVIASIVSLHPKILVLDEPTTALDPTVQALVLDVIRELQEKFKMCIIFITHDLGVVASIANYISIMYAGQIIEEGQRDEILWYPQHPYTWGLIMSMPDVNKGDRLATIKGSVPSRLNDIVGDAFAIRNDYALTRDFEHEPEMYHVSETHRVKSALLDERAEKYTPPQIILDKWNNFKKRQENN" }, "paralog": false, "identity": 0.9826086956521739, "score": 556.0, "evalue": 1.65e-199, "description": "Internal stop codon at 700931. Nonsense mutation around 700931.", "psc": { "uniref90_id": "UniRef90_Q9PPT0", "gene": "DppD", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_A0A084F1H7", "cog_id": "COG0444", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPT0", "UniRef:UniRef50_A0A084F1H7", "COG:COG0444", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084F1H7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084F1H7" ], "product": "Oligopeptide ABC transporter, ATP-binding protein" } }, "id": "GPKLONFDEH_1194", "locus": "GCA000006625_01193" }, { "type": "cds", "sequence": "contig_1", "start": 701658, "stop": 701900, "strand": "-", "frame": 2, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "nt": "ATGATGATTACTCAATTAGTATTTAGTATTCCAGGAGCTATTTTAGCTGAATCATCACTTGCTTTTATTGGTTTATCTTTACCAAATACACCAACTTTAGGTAATTTAATTGCAGATGGTTCATCAATCATTACTTTATATCCACGTTTTACTTTAATTCCATCATTTTTACTAGTTTGCTTAGTTGCTTCAATTCAATTAGTTGGGGGTGCCACACAAGACGCATTAAGACGTCAAAGATAA", "aa": "MMITQLVFSIPGAILAESSLAFIGLSLPNTPTLGNLIADGSSIITLYPRFTLIPSFLLVCLVASIQLVGGATQDALRRQR", "aa_hexdigest": "1e280d2254de147a4616b8aafa112213", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FAT0", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.563, "score": 95.5, "evalue": 2.89e-24, "valid": false, "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_A0A3B0PSU9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PSU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "product": "ABC transmembrane type-1 domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_1195", "locus": "GCA000006625_01194" }, { "type": "cds", "sequence": "contig_1", "start": 702030, "stop": 702179, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATTTTGAAATGTTTGATAAAATCATGACATTCATTATTAAAATTTTAACAAACGTTCCAACAATCATCATTTTAATGATTTTAACAATTATTTTAAAACCATCATTTGGAGTTATGATTTTTGCTATTTCATTTTCTGGTTGA", "aa": "MGYFEMFDKIMTFIIKILTNVPTIIILMILTIILKPSFGVMIFAISFSG", "aa_hexdigest": "693a728eb6ea02449f5d571e9019bd72", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5573.9254999999985, "isoelectric_point": 8.189692878723147 }, "id": "GPKLONFDEH_1196", "locus": "GCA000006625_01195" }, { "type": "cds", "sequence": "contig_1", "start": 702273, "stop": 702494, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGGGCGCCCTCGTCCTTTTCGTGAATTTGCTAACCGATACGGACGTTCTTTTTCTGGATTAATCGGATTAGCAATTATTATTATTTTATTTATTTTGGCAATTATTATTCCTTTTACAACAGGATCACCAACAGAATTACGACCAAATAAACGATATTTAGATTATTTTACAGATGGTTTTATTTTTGGAACTGACAATAATGGGCGTGATTTATGA", "aa": "MGGRPRPFREFANRYGRSFSGLIGLAIIIILFILAIIIPFTTGSPTELRPNKRYLDYFTDGFIFGTDNNGRDL", "aa_hexdigest": "b645ae506f8d1f1572e866f6c1fdcb54", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8271.469199999994, "isoelectric_point": 9.968440055847168 }, "id": "GPKLONFDEH_1197", "locus": "GCA000006625_01196" }, { "type": "cds", "sequence": "contig_1", "start": 702537, "stop": 702656, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCGAAGTTTGGAGAATATTTAAAACAAAAATCAAAAAGTATTACTTCATCATTTATAGATGAATTAACATCTGATGCACCAAATAAGTTTTTACAACCTTTTCAACACCAACAATGA", "aa": "MAKFGEYLKQKSKSITSSFIDELTSDAPNKFLQPFQHQQ", "aa_hexdigest": "f96e3f3a8cdda05c754d4385fa8850a8", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4519.051700000001, "isoelectric_point": 8.120776176452637 }, "id": "GPKLONFDEH_1198", "locus": "GCA000006625_01197" }, { "type": "cds", "sequence": "contig_1", "start": 702661, "stop": 702849, "strand": "-", "frame": 1, "gene": "oppB", "product": "Oligopeptide transport system permease protein oppB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6S4" ], "nt": "TTGTTTATTGAACAAATTTTTCAAATTCCAGGTTCAGGTTCATTATTTTTTGAAGCAATTATCTCAAAAGATTACAACGTGATTTTATTATTAGTAATTATTTATTCATTAATTACAATTATTGGTTATACAATTCGAGATGCTTTATATCAAATTATTGATCCACGTATAAGAAGAGGAGGAAAATAA", "aa": "MFIEQIFQIPGSGSLFFEAIISKDYNVILLLVIIYSLITIIGYTIRDALYQIIDPRIRRGGK", "aa_hexdigest": "6a7cf37ebab260f9358652ee6476caba", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A3B0P6S4", "query_cov": 0.9838709677419355, "subject_cov": 0.8472222222222222, "identity": 0.639, "score": 81.3, "evalue": 4.87e-19, "valid": false, "product": "Oligopeptide transport system permease protein oppB", "uniref50_id": "UniRef50_A0A3B0P6S4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P6S4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6S4" ], "product": "Oligopeptide transport system permease protein oppB" }, "genes": [ "oppB" ], "id": "GPKLONFDEH_1199", "locus": "GCA000006625_01198" }, { "type": "cds", "sequence": "contig_1", "start": 702928, "stop": 703134, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATTAATTGGTAAAGAAGTTGGATTACCATTTACGTTTGATATTAATAATTTTGTGACTTATATTTTACCAGGGTTAGCATTATCACTTGGTTCAATTATTGTTTATGTGCAATATATTAAAGTTGAAATGAATCGTGAATTAAACTCAATGCATGCGAAATTTGCGTATTTAAAAGGTGCAACTGTTAACCGTTTTGTGTGA", "aa": "MLLIGKEVGLPFTFDINNFVTYILPGLALSLGSIIVYVQYIKVEMNRELNSMHAKFAYLKGATVNRFV", "aa_hexdigest": "f23e19575b840ff7aa752d036578ea99", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7683.059699999999, "isoelectric_point": 9.157298851013184 }, "id": "GPKLONFDEH_1200", "locus": "GCA000006625_01199" }, { "type": "cds", "sequence": "contig_1", "start": 703534, "stop": 703794, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAGAAAAACAGCTTTGAAGCTAATTCATCGCAAAACAATTATGGTGAATTGCCAATATTAAACCAAAAAAAACGAGTTAATTATTCATTTAGTAATAATTTTATTATTTTTGGTGGTGGTCGTTCACTAAAAACAGCAGCAGATCGCGATCGTGTTGTTGATCCATTAATCGATTTTTTCACTTTTGATTCAAAGTTTATGACATTTATTATTAAATCTTTGAAATTAATTGCTGAATTTTTCTTATTAGCTTGA", "aa": "MNQKNSFEANSSQNNYGELPILNQKKRVNYSFSNNFIIFGGGRSLKTAADRDRVVDPLIDFFTFDSKFMTFIIKSLKLIAEFFLLA", "aa_hexdigest": "cb758b85525b7c9e7427f9945a589f39", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9894.216599999992, "isoelectric_point": 9.453724479675294 }, "id": "GPKLONFDEH_1201", "locus": "GCA000006625_01200" }, { "type": "cds", "sequence": "contig_1", "start": 703809, "stop": 703979, "strand": "-", "frame": 2, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8B4", "UniRef:UniRef100_A0AAC9X6J3", "UniRef:UniRef50_A0AAC9X6J3", "UniRef:UniRef90_A0AAC9X6J3" ], "nt": "ATGAAATTAGTTGATCGTTTTTATATCAATGCTAAATATGAATTACGATCTATTGATTATGCCACAATTCGCTTAAATATTTTAATTACAATTATCATTTTACCTAGTTTTATTTATGCATTAGCTTGTATGATTGCTTATATTATTAAAACCACACAATCAATTACTTAA", "aa": "MKLVDRFYINAKYELRSIDYATIRLNILITIIILPSFIYALACMIAYIIKTTQSIT", "aa_hexdigest": "11590faf78dfaea745cd27b9518327af", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000165F8B4", "uniref100_id": "UniRef100_A0AAC9X6J3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000165F8B4", "UniRef:UniRef100_A0AAC9X6J3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9X6J3", "uniref90_id": "UniRef90_A0AAC9X6J3" }, "psc": { "uniref90_id": "UniRef90_A0AAC9X6J3", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6J3" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6J3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6J3" ], "product": "DUF3899 domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_1202", "locus": "GCA000006625_01201" }, { "type": "cds", "sequence": "contig_1", "start": 704028, "stop": 704249, "strand": "-", "frame": 2, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6J3", "UniRef:UniRef90_A0AAC9X6J3" ], "nt": "ATGAGTATAAAAAAACAATCGTTTCCGAATCGTTTATTAAATTATAAAAGCATTATTGTTTTAATTGCATTATTAATATTGATGGTTATTAGTCACTTAATTGTTGAATTAATTAAAGATTATCACCAATACCGTATGAATGTTTTTATAGCTTATACATGTATCTTTATGGTAGTTTTCTTATATATTATCTTCTCGCGCCAAGTTTATCGTCGCCGTTGA", "aa": "MSIKKQSFPNRLLNYKSIIVLIALLILMVISHLIVELIKDYHQYRMNVFIAYTCIFMVVFLYIIFSRQVYRRR", "aa_hexdigest": "5c5d7163bb41b6b7885bc2d2902f4ac0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 703809, "stop": 704249, "observations": { "mutation": [ 703985, 704030 ], "stop": [ 703985, 704030 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9X6J3", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 134.0, "evalue": 4.36e-39, "gene_start": 1, "gene_end": 73, "reference_start": 1, "reference_end": 73, "reference_sequence": "MSIKKQSFPNRLLNYKSIIVLIALLILMVISHLIVELIKDYHQYRMNVFIAYTCIFMVVFLYIIFSRQVYRRRWAVKLRKYNEYKLSSWEMKLVDRFYINAKYELRSIDYATIRLNILITIIILPSFIYALACMIAYIIKTTQSIT" }, "paralog": false, "identity": 0.9863013698630136, "score": 275.0, "evalue": 5.25e-97, "description": "Internal stop codon at 703985, 704030. Nonsense mutation around 703985, 704030.", "psc": { "uniref90_id": "UniRef90_A0AAC9X6J3", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_A0AAC9X6J3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9X6J3", "UniRef:UniRef50_A0AAC9X6J3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9X6J3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9X6J3" ], "product": "DUF3899 domain-containing protein" } }, "id": "GPKLONFDEH_1203", "locus": "GCA000006625_01202" }, { "type": "cds", "sequence": "contig_1", "start": 704284, "stop": 704385, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGTTATTTATAGTATGTATAGTTATTTTGATTTAACACTTGAACCTAAAAAACCAGGTAGTATTCATCATTGCCATGCTGATGGTTTTTGTCATTAA", "aa": "MYVIYSMYSYFDLTLEPKKPGSIHHCHADGFCH", "aa_hexdigest": "a805e6a5fb9387a637bf1d75611b968e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3891.4321999999993, "isoelectric_point": 6.363432121276855 }, "id": "GPKLONFDEH_1204", "locus": "GCA000006625_01203" }, { "type": "cds", "sequence": "contig_1", "start": 704566, "stop": 704715, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTAAAATTAATGATTTAAATATTGATAACGATGTTAAATTATTTAATTTAAATGATCTTGTTTTAAAACAAATTTTTATTGTTGATCGTCCTGCACAAACATCTATTAAACCCGAACGTGATCTTGTTTATACCAATATTTGA", "aa": "MISKINDLNIDNDVKLFNLNDLVLKQIFIVDRPAQTSIKPERDLVYTNI", "aa_hexdigest": "6040900ddc5eb75f043937c51dec7d0a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5701.548600000001, "isoelectric_point": 4.919437599182127 }, "id": "GPKLONFDEH_1205", "locus": "GCA000006625_01204" }, { "type": "cds", "sequence": "contig_1", "start": 704764, "stop": 704874, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATTAATTATTCAAATGTCAAAATTTATCATTCGATTATTAAATATCCAGATAGCAAAGAACATTATGAAGGATATACAATAACTGGTGGTAGTTTATATCGTTGA", "aa": "MQINYSNVKIYHSIIKYPDSKEHYEGYTITGGSLYR", "aa_hexdigest": "71098ad6f2a02ec3f98db2fc0c9d6864", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4269.744200000001, "isoelectric_point": 8.048571586608887 }, "id": "GPKLONFDEH_1206", "locus": "GCA000006625_01205" }, { "type": "cds", "sequence": "contig_1", "start": 704923, "stop": 705477, "strand": "-", "frame": 1, "gene": null, "product": "Iron ABC transporter substrate-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T094", "UniRef:UniRef90_A0AAC9T094" ], "nt": "ATGAAAACAAAAAGTAATCTCAAAGCAAGATTAATAATCTTTATTAGTATATTATTAACAACTATTATTAGTTTAGTTTTATTAACTAGTTGTTCAACAAAAAAATCTAAAAAAGTAACAATTAATCAAGATACGCAAATCAAGTTTCAATCAATCACAAATAATAATATTAATCTTGCAAATGAAAAACAAATATTAAATCAATCTCAAAATAAAGAAACTAAAATTTTTCTAAACCTCTATGAGAGTTTAAAAGATTATTTTGATCAAACAATTAAACGCTCCTTGTATTTATATTATTCTTATAATTTTGCTTTACTAAATTTATATAATGCAAAATATCATGTTAAGTTAAATGATTTTAGTTTAATTTACAATACAGATAAAAATTCAAATAATGGTACTTTTGATATTAATTATAAATTAGGCTTAGTTATTGATGACATTGGTGATATTAATATACAAACTAAACCTCAATTTTATGAGCCAATTCCACCTGACAAAAAAGTGAGAAATGATGATTATCACTTTACTAAGAAAATTAAACGTTTTTGA", "aa": "MKTKSNLKARLIIFISILLTTIISLVLLTSCSTKKSKKVTINQDTQIKFQSITNNNINLANEKQILNQSQNKETKIFLNLYESLKDYFDQTIKRSLYLYYSYNFALLNLYNAKYHVKLNDFSLIYNTDKNSNNGTFDINYKLGLVIDDIGDINIQTKPQFYEPIPPDKKVRNDDYHFTKKIKRF", "aa_hexdigest": "9ebc434f305245b388680679887dae9b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 704620, "stop": 705477, "observations": { "mutation": [ 704766, 704925 ], "stop": [ 704766, 704925 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T094", "query_cov": 1.0, "subject_cov": 0.4634760705289673, "identity": 1.0, "score": 347.0, "evalue": 1.68e-117, "gene_start": 1, "gene_end": 184, "reference_start": 1, "reference_end": 184, "reference_sequence": "MKTKSNLKARLIIFISILLTTIISLVLLTSCSTKKSKKVTINQDTQIKFQSITNNNINLANEKQILNQSQNKETKIFLNLYESLKDYFDQTIKRSLYLYYSYNFALLNLYNAKYHVKLNDFSLIYNTDKNSNNGTFDINYKLGLVIDDIGDINIQTKPQFYEPIPPDKKVRNDDYHFTKKIKRFWFGSKKAIIDYFNEAISMQINYSNVKIYHSIIKYPDSKEHYEGYTITGGSLYRWVKQAIPTDDNQNKVRYMISKINDLNIDNDVKLFNLNDLVLKQIFIVDRPAQTSIKPERDLVYTNIWNNKLDDFYFSPIAFLAPTFIKKNGKKITPFNLKTNKEYLDNLYENLLIQGQKNNNALIKGMYVIYSMYSYFDLTLEPKKPGSIHHCHADGFCH" }, "paralog": false, "identity": 0.9929824561403509, "score": 562.0, "evalue": 1.27e-204, "description": "Internal stop codon at 704766, 704925. Nonsense mutation around 704766, 704925.", "psc": { "uniref90_id": "UniRef90_A0AAC9T094", "product": "Iron ABC transporter substrate-binding protein", "uniref50_id": "UniRef50_A0AAC9T094", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T094", "UniRef:UniRef50_A0AAC9T094" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T094", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T094" ], "product": "Iron ABC transporter substrate-binding protein" } }, "id": "GPKLONFDEH_1207", "locus": "GCA000006625_01206" }, { "type": "cds", "sequence": "contig_1", "start": 705567, "stop": 705719, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T1F4" ], "nt": "ATGATTAATTTAAATAATAATCAAAATAAATTTTTTAATCATACCATTCGCATTTTTTATGGCAAACGTATTTTTTGCTCTCGTTTTATTATTATTTGCATTTCTATTATTTATAAGATGTATATTAATACAATTAAAATTAATAAGCGTTGA", "aa": "MINLNNNQNKFFNHTIRIFYGKRIFCSRFIIICISIIYKMYINTIKINKR", "aa_hexdigest": "025c6fa9b00d1c2804fe2c2beb7196b7", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_A0AAC9T1F4", "query_cov": 1.1, "subject_cov": 0.859375, "identity": 0.818, "score": 82.8, "evalue": 5.86e-20, "valid": false, "uniref50_id": "UniRef50_A0AAC9T1F4" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T1F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T1F4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6173.395399999998, "isoelectric_point": 10.463815116882323 }, "id": "GPKLONFDEH_1208", "locus": "GCA000006625_01207" }, { "type": "cds", "sequence": "contig_1", "start": 705907, "stop": 706245, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGCATCAATTCTTGCTTCAATTGTTTTAGGAATTGTTTTTAACATTTATTTTCCAAAACATAAAAGTTTGAAAATTCAAGTTTATTCAAAAAACGTTAATGCTATCATTGAAAATTTACGTGCTAATGATTTTAATAATTCAATTACTTTAAATAGTTTAAGTGATTCATTAACATTAAGAACAAATTATTCATTAGAAATTATTTCTCCATATATTGAGTTACCATCTTTAATTCATTTAGTGCGCGATATTGATAAGAATTGTTTAATAGTGATCTATCCAATTATTGATATTGATGGAGAAATGGTTGTTAAGAAATCTACTATTAGTTAA", "aa": "MVASILASIVLGIVFNIYFPKHKSLKIQVYSKNVNAIIENLRANDFNNSITLNSLSDSLTLRTNYSLEIISPYIELPSLIHLVRDIDKNCLIVIYPIIDIDGEMVVKKSTIS", "aa_hexdigest": "06f95f2e3122e8e17d355b794d00fc68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12593.632699999987, "isoelectric_point": 6.709467887878417 }, "id": "GPKLONFDEH_1209", "locus": "GCA000006625_01208" }, { "type": "cds", "sequence": "contig_1", "start": 706534, "stop": 706908, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTGACATATATTATTACTGTGCAAGTTTTTGGTTTTGCTTTAAGTCAAATTCCAAACGTAGAAAAAATTATGATTTTTGGCGACAACCGTTTAAGTTATCCACCTATTAATTTGTTTTTTACTAATGGTATTTTAGATGAAAAAATAGTTAAAAATATTGAAACTGGTGTCTTGAATTATTCTAATATTACTAATTCAAAAATTGCTGATGAATTTATGCATTATGTACAAGCAAATCCTAATGCGACAATTGGTGATTTATTTCATTTAAACCAAAGTTTGACTTATCGACAATCTTTGTATGAATTTGCTAAGCCCTTATATATTTTTCATGATCTTATTATTAATAAAGTACAAATTTTATCTTGA", "aa": "MTLTYIITVQVFGFALSQIPNVEKIMIFGDNRLSYPPINLFFTNGILDEKIVKNIETGVLNYSNITNSKIADEFMHYVQANPNATIGDLFHLNQSLTYRQSLYEFAKPLYIFHDLIINKVQILS", "aa_hexdigest": "52cccbf367c31b3ddc46bcdd5d7939a2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14250.298099999982, "isoelectric_point": 5.821529197692871 }, "id": "GPKLONFDEH_1210", "locus": "GCA000006625_01209" }, { "type": "cds", "sequence": "contig_1", "start": 706981, "stop": 707082, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAACATCACAAGGGATCGCACGAATTTTACAATCAATTATGATTTCAAATCATAGTTCACCAAAAGAAGCCAAATTAGCATACGATATTATGTTTTGA", "aa": "MGTSQGIARILQSIMISNHSSPKEAKLAYDIMF", "aa_hexdigest": "512c2bd4f28003a02a85091d37342755", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3639.2273999999998, "isoelectric_point": 8.275113487243654 }, "id": "GPKLONFDEH_1211", "locus": "GCA000006625_01210" }, { "type": "cds", "sequence": "contig_1", "start": 707526, "stop": 707897, "strand": "-", "frame": 2, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "RefSeq:WP_006688591.1", "SO:0001217", "UniParc:UPI0000133CCE", "UniRef:UniRef100_Q9PPS3", "UniRef:UniRef50_Q8EWU9", "UniRef:UniRef90_Q9PPS3" ], "nt": "ATGGCTTTATTTAAGATTAACAAAGGCGAAATTATGAATTTTGTTAATTCAACACAATTAAAAACAGATATTCCTAATTTTGATTCTGGTGATACAATTATCGTTCATAATCGAATTGTAGAAGGAAAAAAATCACGAATTCAAAAGTTTGAAGGCGTTGTTTTACGTCGTCGTGGTTCTGGTTCAAGTGAAACAGTTATTGTTCGTAAAGAATCAAGTGGTATAGGTGTTGAACAATCATTCAATATTCATTCTCCACTTGTTGAAAAAATTGAAGTTATCAAGTATGGTAAAGTAAGACGTGCTTATATTTCATACATGCGTAATCGTTCAGGCAAATCTGCAAGAATTAAAGAATTAAATAAACAATAA", "aa": "MALFKINKGEIMNFVNSTQLKTDIPNFDSGDTIIVHNRIVEGKKSRIQKFEGVVLRRRGSGSSETVIVRKESSGIGVEQSFNIHSPLVEKIEVIKYGKVRRAYISYMRNRSGKSARIKELNKQ", "aa_hexdigest": "134ea9784a49d661660ac92647524c7f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0000133CCE", "ncbi_nrp_id": "WP_006688591.1", "uniref100_id": "UniRef100_Q9PPS3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133CCE", "RefSeq:WP_006688591.1", "UniRef:UniRef100_Q9PPS3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPS3", "uniref90_id": "UniRef90_Q9PPS3" }, "psc": { "uniref90_id": "UniRef90_Q9PPS3", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_Q8EWU9", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EWU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EWU9" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "GPKLONFDEH_1212", "locus": "GCA000006625_01211" }, { "type": "cds", "sequence": "contig_1", "start": 707907, "stop": 708593, "strand": "-", "frame": 2, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "db_xrefs": [ "COG:COG0336", "COG:J", "EC:2.1.1.228", "GO:0002939", "GO:0005829", "GO:0030488", "GO:0052906", "KEGG:K00554", "RefSeq:WP_006688650.1", "SO:0001217", "UniParc:UPI0000137433", "UniRef:UniRef100_Q9PPS2", "UniRef:UniRef50_Q6F0S5", "UniRef:UniRef90_Q9PPS2" ], "nt": "ATGAAAATATCGATTTTATCTTTATTTCCTGAATTATATGAAACATGGATAAAGCATTCAATTATTAGTAATGCTATTAAAAATAACCAAGTTATAATAGAAGTTATCGATTTTCGTTTATATACAAATGATAAACATAAAAAAGTTGATGATTACCAATATGGTGGCGGAGCAGGTATGGTTTTAAGGATTGAACCAATTGTTGCTGCCATTCGTGCAATTCGTTCTTCTAATTCATATGTGATTTTAACAACACCAAAAGGTCAAGTTTTTAATCAAGAATTAGCAAACAATTTTGTTAGTAAATATGATCATATCATCATTATTGCTGGTCATTATGAAGGTTTTGATGAACGTATTAATTATTACATTGACGCCCAATATAGTATCGGTGATTTTGTCTTAACAGGGGGGGAATTACCAAGTATGGTAATTTCTGATGCTGTTATTCGTTTGTTAGATGGCGTTATTTCTCCTTCTAGTTTAGAAACTGAATCATTTAATAACTACTTATTAGATTATCCTGTTTACACACGCCCTCTTGTTTTTGAAGGGCATAAAGTACCGGATATACTATTATCTGGTCATCATAAAAATATTGCTGATTTTCGTAAACAGCAACAAGAGACGATTACTAAAAAGAATCGTCCTGATTTATATCAAAAATATTTAAATAACAAAAAGTAG", "aa": "MKISILSLFPELYETWIKHSIISNAIKNNQVIIEVIDFRLYTNDKHKKVDDYQYGGGAGMVLRIEPIVAAIRAIRSSNSYVILTTPKGQVFNQELANNFVSKYDHIIIIAGHYEGFDERINYYIDAQYSIGDFVLTGGELPSMVISDAVIRLLDGVISPSSLETESFNNYLLDYPVYTRPLVFEGHKVPDILLSGHHKNIADFRKQQQETITKKNRPDLYQKYLNNKK", "aa_hexdigest": "3fd9c8fd73f843c65de0b600f7534a25", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137433", "ncbi_nrp_id": "WP_006688650.1", "uniref100_id": "UniRef100_Q9PPS2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137433", "RefSeq:WP_006688650.1", "UniRef:UniRef100_Q9PPS2" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPS2", "uniref90_id": "UniRef90_Q9PPS2" }, "psc": { "uniref90_id": "UniRef90_Q9PPS2", "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_Q6F0S5", "cog_id": "COG0336", "cog_category": "J", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0002939", "GO:0005829", "GO:0030488", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_Q6F0S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6F0S5" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [ "trmD" ], "id": "GPKLONFDEH_1213", "locus": "GCA000006625_01212" }, { "type": "cds", "sequence": "contig_1", "start": 708672, "stop": 708902, "strand": "-", "frame": 2, "gene": null, "product": "Small ribosomal subunit protein bS16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A2RJS1" ], "nt": "TTGGTAAAGATTAGATTAACAAGAGTAGGAACACATAAAAAACCATTTTTTCGTATTGTAGTTATGGATGCTAAAACCAAAGCTAATGGTGCATATATTGAAAATTTAGGTCATTATGATCCTGTTTTAGGACAAGTTGTTTTAAAAAAAGAAGCTATTTTAGCTCAATTACAAAACGGAGCACAACCTAGTGAAACGGTAAAAAATATCTTATCACAAGAAGGTATTTGA", "aa": "MVKIRLTRVGTHKKPFFRIVVMDAKTKANGAYIENLGHYDPVLGQVVLKKEAILAQLQNGAQPSETVKNILSQEGI", "aa_hexdigest": "b2e9518415e3cee2360d06483aea9a4b", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A841CAP4", "query_cov": 1.013157894736842, "subject_cov": 0.8461538461538461, "identity": 0.5579999999999999, "score": 89.0, "evalue": 1.29e-21, "valid": false, "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_A2RJS1", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A2RJS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A2RJS1" ], "product": "Small ribosomal subunit protein bS16" }, "genes": [], "id": "GPKLONFDEH_1214", "locus": "GCA000006625_01213" }, { "type": "cds", "sequence": "contig_1", "start": 708998, "stop": 709483, "strand": "-", "frame": 3, "gene": "cof", "product": "Cof-type HAD-IIB family hydrolase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0AAC9T2T4", "UniRef:UniRef90_A0AAC9T2T4" ], "nt": "ATGCAAACACATTTTTTAGATGTTTATAATATTAACTATAAATTTTTATTACAACTTCATCACACAAAAAGAAAAGTTTGTTTAAACCCAATTTTTAATAAAAATGATAAATTTTATAAAATTAATATTTTACCTTCAAATTTTGTTAAAAAATTAAAACACGAGGTTATAGATCAATTGATTAAAGAATTTAGTGATCAAGTAAATATTTCATTCACCTCAAAATACATTGTTGAAGTGACTAATAAAAATATTAATAAGGCATCATCATTACGATTTATTGCTAATTTATACCAGATTAATTTAGATAAGGTTGCAACGATTGGCGATTCGCCTAATGATATTCCAATGTTTAAAATTAGTGGTTTAGCAGCGGCTGCTAGAACTAAATCTAAAACAATTATTGAACATGTTGATGTGATTATTAATTATAAAAATAATTCTAATTCTGTTGCATTATTTATTAATAATTACTTACTAAAATAA", "aa": "MQTHFLDVYNINYKFLLQLHHTKRKVCLNPIFNKNDKFYKINILPSNFVKKLKHEVIDQLIKEFSDQVNISFTSKYIVEVTNKNINKASSLRFIANLYQINLDKVATIGDSPNDIPMFKISGLAAAARTKSKTIIEHVDVIINYKNNSNSVALFINNYLLK", "aa_hexdigest": "79090405daa840d8e9cb59be89908c90", "start_type": "ATG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 708998, "stop": 709783, "observations": { "mutation": [ 709504 ], "stop": [ 709504 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T2T4", "query_cov": 1.0, "subject_cov": 0.5812274368231047, "identity": 1.0, "score": 315.0, "evalue": 5e-107, "gene_start": 1, "gene_end": 161, "reference_start": 117, "reference_end": 277, "reference_sequence": "MQYKMLVIDLDGTLLTKTKNISKANLQGLKKYISLGGKVVLSTGRSLENTLKIVHLIHDQIKELIQYISCFNGSYIYDVINEKVLFESTINKDIVNEIYKFSLKHGLGFWPYNQRFMQTHFLDVYNINYKFLLQLHHTKRKVCLNPIFNKNDKFYKINILPSNFVKKLKHEVIDQLIKEFSDQVNISFTSKYIVEVTNKNINKASSLRFIANLYQINLDKVATIGDSPNDIPMFKISGLAAAARTKSKTIIEHVDVIINYKNNSNSVALFINNYLLK" }, "paralog": false, "identity": 0.9961685823754789, "score": 514.0, "evalue": 3.44e-188, "description": "Internal stop codon at 709504. Nonsense mutation around 709504.", "psc": { "uniref90_id": "UniRef90_A0AAC9T2T4", "gene": "Cof", "product": "Cof-type HAD-IIB family hydrolase", "uniref50_id": "UniRef50_A0AAC9T2T4", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T2T4", "UniRef:UniRef50_A0AAC9T2T4", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2T4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2T4" ], "product": "Cof-type HAD-IIB family hydrolase" } }, "id": "GPKLONFDEH_1215", "locus": "GCA000006625_01214" }, { "type": "cds", "sequence": "contig_1", "start": 709502, "stop": 709831, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGCAATATAAGATGTTAGTTATTGATCTAGATGGTACTTTATTAACGAAAACAAAAAACATTAGTAAAGCTAATTTACAAGGATTAAAAAAATATATTAGTTTAGGCGGTAAGGTTGTACTAAGTACAGGACGTTCATTAGAAAATACTTTAAAGATTGTGCATTTAATTCATGATCAAATCAAGGAATTAATCCAATATATTTCATGTTTTAATGGCTCATATATTTATGATGTTATTAATGAAAAAGTATTGTTTGAATCAACAATTAATAAAGATATAGTGAATGAAATCTATAAATTTTCATTAAAACATGGATTAGGATTTTGA", "aa": "MQYKMLVIDLDGTLLTKTKNISKANLQGLKKYISLGGKVVLSTGRSLENTLKIVHLIHDQIKELIQYISCFNGSYIYDVINEKVLFESTINKDIVNEIYKFSLKHGLGF", "aa_hexdigest": "190b62690c46d74f3f500d7179ea3241", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 102, "aa_cov": 0.9357798165137615, "hmm_cov": 0.9411764705882353, "evalue": 3.843334256833979e-25, "score": 84.6131820678711, "start": 6, "stop": 107 } ], "seq_stats": { "molecular_weight": 12410.42999999999, "isoelectric_point": 9.1631010055542 }, "id": "GPKLONFDEH_1216", "locus": "GCA000006625_01215" }, { "type": "cds", "sequence": "contig_1", "start": 709862, "stop": 709975, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAACAGTTTAGAAAAGACAATAATTATGTTGAATTTATCAAACAAGCTGAAGTTGAAGAAATTGAACAAGAAGTTGATATTGATGATTTAATTTCAAACGGTTTATAA", "aa": "MVEQFRKDNNYVEFIKQAEVEEIEQEVDIDDLISNGL", "aa_hexdigest": "ca3ef550d8d6e37edbd9c874352a9dad", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4400.781499999999, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_1217", "locus": "GCA000006625_01216" }, { "type": "cds", "sequence": "contig_1", "start": 710027, "stop": 711652, "strand": "-", "frame": 3, "gene": "rnjA", "product": "Ribonuclease J", "db_xrefs": [ "COG:COG0595", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A084EWI6", "UniRef:UniRef90_A0AAC9T1X3" ], "nt": "ATGGAAAATACCAAAAAAAGTCCTACATATGTTTATGCTTTAGGGGGGCTAGAAGAAATTGGTAAAAACACTTATGTTGTTGAACATGAAGATGAAATTATTTTAATTGATGCGGGCATTAAATTTGCTAATGCATCTTTACCAGGATTTGATGGAACTGTTGCCAATTTTGATTATTTAATAAAAAACAATCATAAAATTCATTCATTAGTAGTTACTCATGGTCATGAAGACCATATTGGGGGGATTCCTCACATTTTGCGACATGTTAAAATTAAGACTATCTATGCACCAACATTAGCAGCAAAATTAATTGAACGTCGTTTAAGTGAATATAAAGATATTAAACCTCCGCGTATTATTGTCTTTGAAGATGAGTCAATGTATAAAACAAAATATTTTGAAGTTGATTTTTATCGTGTTTGTCATTCAATTCCTGATTCATTTGGAATTTGTGTAAAAACACCAAACGGATACATTGTAACAACTGGTGATTTTAGATTTGATTTTGCTACAGCTGGTGATGAGACTAATTTAGCTAAGATTTCACAAATTTCTAATCGTGGAATCTCAGTATTAATGTGCGAATCAACAAGTGCTGAAATTCCTGGATTTAGTGAAAGTGAACGATATGTTATTGATAACATTCGTGATTATATGGTAAATATTAAAGGACGAACTTTTATTTCAACATTTGCTTCTAATTTAGGTCGTGTTGAAGAAATTATTGCTATAGCTGTTGGTTTAAATAAAAAAATCTGTATCATTGGTAAATCAATGGAAGCTAATATTAAAACAAGTCGAAAATTGGGTTATTTAAATGTTCCTGAATCTTCATTTATCACACATAAAGAATTGCCATTTTATAAAGATCATGAAATTGTTGTAATTTTAACTGGTTCACAAGGTGAAAAAATGGCGGCTTTAAATGTTATGGCTAATAATAACCACTCAAAAATTACTTTAAAACCATCAGATACAATTATCTTATCATCAAATCCAATCCCAGGAAACTATGCTCAAGTCGAAGCAATGGTTAATAAACTTTATAAACTCGGATTAACAGTTTATGAAAATTCACCTAACAAAAAGATTCATGCTTCTGGTCATGCCACACGGAGTGAACACCAATTAATGATTAAAGCAATTAATCCATCATATTTATTTCCAATCCATGGGGAATATAAAATGTTTCGTGCTTTAAAACAAAATGCTGTTGACCAAGGTTTTGATAAAGACCATGTCATTATTGCACCAAATGGCCAAAAATTACAATTATTAGATGGTGTTTTAAGTCATACTAATATTTATGTTGATGCTGAACCTAAATTTATTAATGGTTATGAAATTAGTTCTAAAATTAGTAAATTATTAAGTGAACGTGTTGTTTTATCCAGTGATGGTATTTTAAATCTAGTTTTAAATGCTGATTTTAAAAAAGCTAAGTTAAACTCTGCTGTAAGTATTTCAACACGAGGATGTTTTTTTGCCAAAGAATCAACTAATTTAATTAATAAAATTTCTAATGTAGCAAAATCAAGTTTAGAAGAAGCGTTGGCAAAAAAAGAATTTGATGAAAAAAAACTAAAAGAAATAGTTAGTGGAAATGTTAAATCAATTGTTTGA", "aa": "MENTKKSPTYVYALGGLEEIGKNTYVVEHEDEIILIDAGIKFANASLPGFDGTVANFDYLIKNNHKIHSLVVTHGHEDHIGGIPHILRHVKIKTIYAPTLAAKLIERRLSEYKDIKPPRIIVFEDESMYKTKYFEVDFYRVCHSIPDSFGICVKTPNGYIVTTGDFRFDFATAGDETNLAKISQISNRGISVLMCESTSAEIPGFSESERYVIDNIRDYMVNIKGRTFISTFASNLGRVEEIIAIAVGLNKKICIIGKSMEANIKTSRKLGYLNVPESSFITHKELPFYKDHEIVVILTGSQGEKMAALNVMANNNHSKITLKPSDTIILSSNPIPGNYAQVEAMVNKLYKLGLTVYENSPNKKIHASGHATRSEHQLMIKAINPSYLFPIHGEYKMFRALKQNAVDQGFDKDHVIIAPNGQKLQLLDGVLSHTNIYVDAEPKFINGYEISSKISKLLSERVVLSSDGILNLVLNADFKKAKLNSAVSISTRGCFFAKESTNLINKISNVAKSSLEEALAKKEFDEKKLKEIVSGNVKSIV", "aa_hexdigest": "3cb4053117f8a2a41cb7f02cf84fc5f9", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0AAC9T1X3", "query_cov": 1.0, "subject_cov": 0.9077181208053692, "identity": 0.98, "score": 1040.0, "evalue": 0.0, "valid": true, "gene": "RnjA", "product": "Ribonuclease J", "uniref50_id": "UniRef50_A0A084EWI6", "cog_id": "COG0595", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A084EWI6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EWI6" ], "product": "Ribonuclease J" }, "genes": [ "rnjA" ], "id": "GPKLONFDEH_1218", "locus": "GCA000006625_01217" }, { "type": "cds", "sequence": "contig_1", "start": 712183, "stop": 712647, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "ATGAATATATTTGAAAAGGGATTGTGTGACAAAATTGAAAATAATGAAATTGTGATAACTAATCTTGAAAATGTTCAGGGTAACGAAGGTGATTTAGTTATTTTATCAATTGCTTATGGCCCAAATCCTGAAGGAAATTTACGTAATAATTTTGGACCACTTAATGCTAAGGGCGGAATGAATCGTTTAAATGTAGCGATCACTCGTGCACGTAAAAAAATGATTGTTATAAAATCACTTTATGGTCATCAAATTCAAGTTTCAAATCTTAATAATCAAAATGCACTGACATTTAAACGCTTTATTGAATATGTTGATCGAATTAATGGTCAATTAAGTATTAATGATTCACTTGAAAGTTTAGAACAACAAACATATTTGGAATTTGATAATGATTTAGTTAAAGAAATCTATAGCGAACTAACAAAAAAATTATCAAATAAATACCAAATCTTTCCAAATTGA", "aa": "MNIFEKGLCDKIENNEIVITNLENVQGNEGDLVILSIAYGPNPEGNLRNNFGPLNAKGGMNRLNVAITRARKKMIVIKSLYGHQIQVSNLNNQNALTFKRFIEYVDRINGQLSINDSLESLEQQTYLEFDNDLVKEIYSELTKKLSNKYQIFPN", "aa_hexdigest": "4c2d1db31369bcd890d2666c2f0eabc2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 65, "aa_cov": 0.42207792207792205, "hmm_cov": 0.8769230769230769, "evalue": 3.5475509801701885e-10, "score": 35.326683044433594, "start": 13, "stop": 77 } ], "seq_stats": { "molecular_weight": 17566.7526, "isoelectric_point": 5.8835973739624015 }, "id": "GPKLONFDEH_1219", "locus": "GCA000006625_01218" }, { "type": "cds", "sequence": "contig_1", "start": 712939, "stop": 713115, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTGAACGATCATATCCTATTGTGTATCGTTGTAAAACTAAAGTTGTCTCTGGTGATGATAAACAATTAAAGCCAACATCATTCTTTATTAATAAGTTAGCTGATAGTGACTTTGAAATTGATGATTTTGATAAAGTAGATTCTTTATTAGAACGTGCTAAAACTTCATGA", "aa": "MTIERSYPIVYRCKTKVVSGDDKQLKPTSFFINKLADSDFEIDDFDKVDSLLERAKTS", "aa_hexdigest": "1e2d5c71d24398821a42b045a0df4e72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6705.5337, "isoelectric_point": 5.194254112243651 }, "id": "GPKLONFDEH_1220", "locus": "GCA000006625_01219" }, { "type": "cds", "sequence": "contig_1", "start": 713263, "stop": 713463, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCTGAAACTAAAGGGCGTTTATTGCAAGATATTATTATTCAATTAAGACGTGAATCAATGCGCTCCGCTAAAATTGTTGGTGAAATTGTTTTTAAAAAATATATTAATAATTTAAGAAATTATTTAACAAAACTACCTCAACAAGAAAAAGATGAAATTACTAACGCATTACGAATTGCTTCATCACGTTCTTGA", "aa": "MFSETKGRLLQDIIIQLRRESMRSAKIVGEIVFKKYINNLRNYLTKLPQQEKDEITNALRIASSRS", "aa_hexdigest": "ba752e63339fe9bf8fc12ddd260f6ed0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7753.959999999998, "isoelectric_point": 10.197044944763181 }, "id": "GPKLONFDEH_1221", "locus": "GCA000006625_01220" }, { "type": "cds", "sequence": "contig_1", "start": 713635, "stop": 713751, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACCAAATTGCTTCGTATCGTGAATTTATCAACCAGGATGTTGATTTTTTGTCAAAATTACATACGCTTGCTGAAATTTATACACCAACAATGCAAGATATTATTCGTGAATGA", "aa": "MDQIASYREFINQDVDFLSKLHTLAEIYTPTMQDIIRE", "aa_hexdigest": "c344a0b52433001abc7b5573b21cf4a9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4546.0952, "isoelectric_point": 4.516164970397949 }, "id": "GPKLONFDEH_1222", "locus": "GCA000006625_01221" }, { "type": "cds", "sequence": "contig_1", "start": 714004, "stop": 714438, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAAATTGAAGATTTATTTACAGCTTATCTAAGATTTACTTCTTTTATTAAAAAATATAAGCTAACAGATAATTATAGTTCTATAGAAATTTTAAAACATTTAAAAATGATTACCAATAAAATTCAAACCAATGATGAATTAGTGACAAAATTTTTGATGCATGCTAATAGAATCACCAAAGACATTGATAATTATTACAACTTTTTAGCTGAACATAATCTTGAATCGAACACTATTTTTATGAATAAATCAATTCGTGATAAACGTATTTTTATAGACAAAATTGGTGATTATTTAAAGTTCCGTAAAGATGTGATAGATAAAGATTTTAGTTTGCAATCAAAAACAACACAAGAACTAAATGAAATTATTGATTTATGTGATAATTTTTTTACAAAACACAAAAAATTATTAGCCAAAAATGAATGA", "aa": "MLKIEDLFTAYLRFTSFIKKYKLTDNYSSIEILKHLKMITNKIQTNDELVTKFLMHANRITKDIDNYYNFLAEHNLESNTIFMNKSIRDKRIFIDKIGDYLKFRKDVIDKDFSLQSKTTQELNEIIDLCDNFFTKHKKLLAKNE", "aa_hexdigest": "e00d3d1fadb8de5d70b17bab52f6bc31", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17301.843999999994, "isoelectric_point": 9.025589942932129 }, "id": "GPKLONFDEH_1223", "locus": "GCA000006625_01222" }, { "type": "cds", "sequence": "contig_1", "start": 714655, "stop": 715551, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGAAAGGAAAATTATTACCAAACGATCCTTTTAAATCACCATTAACTTTATTTAAAGTTGAAATTCGAGAAGAAGCTGATAAAATTTTTATCGCTAAAATTCAAGATGAGCCAATTGTTAATGAAAAAATCCAAATCTTTTTAAAAAAAGGTTATCAAGATTTTAAGGTTGAAACAACAGAATTGTTGACTAATTATGAATTGTCTTCAACTATTAAAATGATTGAAGATATGACAGGTCAAAAAATTAATATTAACGAAAACGAATTTGTTCCTTTCATGAACGAAAATGAGACACAAATTTTAGATCGATATACAACTTTTGAAATTGAACCATCAGCTGTACTGGGGTTATTCGAACCCGATGGGGGCGCTTTAAAAGCTGATTTACATAAAATTATTGAAATGGATGTTGATCCATTTGAATCAGAAAACGATGGTTTGAATAAACCAGTTGAATATTATGAAGATAAAGTAATTAAAGAACAAATGGTGGTGGAAATTGGACGTCCTCTAAATATTTTTCAAAAATATGCTGTTGCATCATCACTTTCACAAAATACTTTGATTTATGGACCTCCTGGAACAGGAAAGTCTGAAGTAATTGCAAATATTATCTTTAATGTTTTATTAAAAGGAAAAAGTAGTTTATTAGTATCAGAAAAACGTGCGGCTTTAGATGTATTAACAGAAAGAATAGGTTCGCTTTCACAATTTGCTTTATATGTTTATGATTTAACAAACAAAGAAACTTTTTTTGAAAAAATTTCTAATTTAAATGATTTATTAGGTACACAATGGTATCGTGAACAATCTCGTTCTACAAAGATTAAAGAAATAGAACCAATTAAATTTACTCCAGAGGAATCAATGTTTTTTAAAAATTATGAGGATTGA", "aa": "MKGKLLPNDPFKSPLTLFKVEIREEADKIFIAKIQDEPIVNEKIQIFLKKGYQDFKVETTELLTNYELSSTIKMIEDMTGQKININENEFVPFMNENETQILDRYTTFEIEPSAVLGLFEPDGGALKADLHKIIEMDVDPFESENDGLNKPVEYYEDKVIKEQMVVEIGRPLNIFQKYAVASSLSQNTLIYGPPGTGKSEVIANIIFNVLLKGKSSLLVSEKRAALDVLTERIGSLSQFALYVYDLTNKETFFEKISNLNDLLGTQWYREQSRSTKIKEIEPIKFTPEESMFFKNYED", "aa_hexdigest": "b8d9a627180b8df46607b0c5315c51b4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 73, "aa_cov": 0.21476510067114093, "hmm_cov": 1.0, "evalue": 3.042003920779002e-09, "score": 32.567447662353516, "start": 172, "stop": 235 } ], "seq_stats": { "molecular_weight": 34229.70400000003, "isoelectric_point": 4.764665031433105 }, "id": "GPKLONFDEH_1224", "locus": "GCA000006625_01223" }, { "type": "cds", "sequence": "contig_1", "start": 715576, "stop": 715995, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGCGATTAAACAAAAATTGATGAATCTTGTTAATTTAAATCCGCATGATTCAATTATTTTAACAAGACTTTCATCATCAAGAAATATAGACTTATTTAAGTATATTAAAAAAGAAGATCTAATTAGTTTTGTTAATGGCGAAATTAGTTATGTTGAATTAAATGTAGGTAAGAATTTAAATTTATTTGAAGAAAGTTTAAAAAATGCTTTAAATATTGGTGAATTTTTAGAAATCTTAGTAAATTACAATGTAAATTTAGCAGATAATAAAATTAATTTACTACAACGTGATTTTCATGCAAATAAGAAAAAAATTATTCCTTTAGCAATTGAACAATTGTTAGCATTTAGAAAAAAGTTTATTTCTATTAATAGACTAGCAAAAAATTATTTTGAAGATACAACTGTTTGA", "aa": "MKKAIKQKLMNLVNLNPHDSIILTRLSSSRNIDLFKYIKKEDLISFVNGEISYVELNVGKNLNLFEESLKNALNIGEFLEILVNYNVNLADNKINLLQRDFHANKKKIIPLAIEQLLAFRKKFISINRLAKNYFEDTTV", "aa_hexdigest": "a8f62a4b4606102e00b10e683103dbdf", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16137.689499999988, "isoelectric_point": 9.521416282653806 }, "id": "GPKLONFDEH_1225", "locus": "GCA000006625_01224" }, { "type": "cds", "sequence": "contig_1", "start": 716191, "stop": 716349, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATAATTTATCTGATTTGGAAATCGAAAAATTGACCAAAACATTAGTATCAAAAAACAAAAGAGTTGTAGCTTTATATGGCGTTCCCGAAAACACTTCTTATAAACTAATGAACGATCTAATTAGTTTAATGGATGTAGAAATTCCTAATTGA", "aa": "MRNNLSDLEIEKLTKTLVSKNKRVVALYGVPENTSYKLMNDLISLMDVEIPN", "aa_hexdigest": "c917723a9b1a2d83135e1039ff7186e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5951.885499999999, "isoelectric_point": 6.108509254455566 }, "id": "GPKLONFDEH_1226", "locus": "GCA000006625_01225" }, { "type": "cds", "sequence": "contig_1", "start": 716440, "stop": 717708, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYI4", "UniRef:UniRef90_A0A2C9DYI4" ], "nt": "TTGCCTTCTAATAACCCTTTAATTAATTGGTGGAAAAGACGCCGTTTAAATACTGTTGTTAAAAAGATTGAAGAAATCAACTCAGCGTTCGCAGACCGTGAAATAACATCTCGTTCAAAAATCTTTTTGATGCCTGTTTTAACAGCATTTAGTACTTTAGGAACTACACTAGCTGTTCCTTTAATATCTGTTACTTTTTTACGGGGTGATGCAATTATTAATTTGTGGGGTAAAGATGGATATATTGCTTTCTTAAGTTTACTATTTTCTTTAGTTGTTGCTGTTATTATTTCAGCTTTTGTCGCTTTATTTAGTAGTTTAAAAAATAATAAACGTAATTATATTGTTTCAAACATGACCAAAGGTCTTAAAAGAATATATGATAAATATTTTATCGAAAGTAATTCTCAAGAAAAGATTAATGCTAAAGTAACTTTTTATTCACGCTTTTTAGAACGTTCTAAATTCGTTGTTCAAAATAATTACACATTTTTTTATGATGTTGTAGATATTAATAGTGATCAATATCCTAAAATATTAAAATATTTCAAAACCTTAAACCAATTAAATAATACAGTAATCTTCGATGCTAGTGGTTTTAAATATTTAGACGAACGTAAAATTTTCCGTAACATTATCGATTTAGAGAAAACAAATGTTGTCCGTTTGGATCGTTATAAAACAAAAACAAGCGGACGTCGCTTAATGAGTTTTATTTTCTATCAATTATCAATCATTGCTAATGTTAATACACGAAAACTTTTACAAAAATTTCCTTTCTTTGTCAACTCTTTATATCGTTTTTTAGATTACAGCGAAAAAAATACTGAGTTATTAACACTTTTATTAGACATTAAAAAACACGCTTCAAAAACAGAAATTCCATTAAACGATGAATCACAATTATTTTTTGTAGACTTTTTTACATTCGTGGTTTTTAAAGCTTTGGATGAATTAGGATTTGAGACGCTAATGAATGATTTAACAGTCTATGGTCGTCCATCTGAAATCACTAAGAAAAATATAACTTATAATTCATTAAAATTGGATTACATCATTAATCGTAATGCGCGTAATTTTGGACAACAAGCATTATTGTTTAACTTATTAGATTATTTTGATGAGACAGTAAATAAAAATATCTTTAACGAATTAGGAAATAGTAATAAAAACATGATTTTTTCTAAAAATCATCAATTAAATTTAGCCAATGAAGCTTTAAGTAAAAAAGGTTTTACAAAGCGTGAAATTGATTTAAATAATCATTGA", "aa": "MPSNNPLINWWKRRRLNTVVKKIEEINSAFADREITSRSKIFLMPVLTAFSTLGTTLAVPLISVTFLRGDAIINLWGKDGYIAFLSLLFSLVVAVIISAFVALFSSLKNNKRNYIVSNMTKGLKRIYDKYFIESNSQEKINAKVTFYSRFLERSKFVVQNNYTFFYDVVDINSDQYPKILKYFKTLNQLNNTVIFDASGFKYLDERKIFRNIIDLEKTNVVRLDRYKTKTSGRRLMSFIFYQLSIIANVNTRKLLQKFPFFVNSLYRFLDYSEKNTELLTLLLDIKKHASKTEIPLNDESQLFFVDFFTFVVFKALDELGFETLMNDLTVYGRPSEITKKNITYNSLKLDYIINRNARNFGQQALLFNLLDYFDETVNKNIFNELGNSNKNMIFSKNHQLNLANEALSKKGFTKREIDLNNH", "aa_hexdigest": "424616cddbcf8cdb4a5f2bf108d8f1a3", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 716191, "stop": 717927, "observations": { "mutation": [ 716347, 717706 ], "stop": [ 716347, 717706 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2C9DYI4", "query_cov": 1.0, "subject_cov": 0.7301038062283737, "identity": 0.917, "score": 746.0, "evalue": 2.96e-268, "gene_start": 1, "gene_end": 422, "reference_start": 84, "reference_end": 505, "reference_sequence": "MRNNLSDLEIEKLTKTLVSKNKRVVALYGVPENASYKLMNDLISLMDVEIPNWSSISSATEDQCINDIKNFLLSNASLENFKNLPSNSPLINWWKKRRLNTVVKKIEEINSAFADREITSRSKIFLMPVLTAFSTLGTTLAVPLISVTFLRGDAIINLWGKSGYIAFLSLLFSLVIAVIISAFVALFSSLKNNKRNYIVSNMTEGFKRIYDKYFIDGNSEERINAKVTFYSRFLERSKLVVQNNYSFFYDVVDINSEQYPKMLKYFKTLNQLNNTVIFDASGFKYLDERKIFRNIIDPEKTNVVRLDRYKTKTSGRRLMNFIFYQLSIIANVNTRKLLQKFPFFVNSLYRFLDYSEKNTELLTLLLDLKKHASKTQVPLDDESQLFFVDFFTFVVFKALDESGFETLINDLTVYGRPSEITKKNITYNSLKLDYIINRNARNFGQQALLFNLLDYFDETGNKNIFNELGNSNKNMIFSKNHQLSLANEALSKKGFTKREIDLNAHWCDALYSNLHDDEDMFVKVIEIKENIDVLSALDEVFVRAQNENVKNLLIYVFNVKMLYCLIDNEYELVNESII" }, "paralog": false, "identity": 0.916955017301038, "score": 1046.0, "evalue": 0.0, "description": "Internal stop codon at 716347, 717706. Nonsense mutation around 716347, 717706.", "psc": { "uniref90_id": "UniRef90_A0A2C9DYI4", "uniref50_id": "UniRef50_A0A2C9DYI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2C9DYI4", "UniRef:UniRef50_A0A2C9DYI4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2C9DYI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2C9DYI4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 49359.426, "isoelectric_point": 9.68336086273193 }, "id": "GPKLONFDEH_1227", "locus": "GCA000006625_01226" }, { "type": "cds", "sequence": "contig_1", "start": 717748, "stop": 717927, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTAAAATTATTGAAATTAAAGATAATGTTGATATCTTAAATGTATTAGATGAACTCTTTGTTCGTGCTCAAAATGATGGTGTTAAAAATTTATTAATTTATGTTTTTAATGTAAAAATGTTATATTCATTAATAGATAATGAATACGAGTTGGTTAATGAGTCAATTATTTAA", "aa": "MFVKIIEIKDNVDILNVLDELFVRAQNDGVKNLLIYVFNVKMLYSLIDNEYELVNESII", "aa_hexdigest": "c7c92405e01e59f540867640078872f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6934.030999999997, "isoelectric_point": 4.278464317321778 }, "id": "GPKLONFDEH_1228", "locus": "GCA000006625_01227" }, { "type": "cds", "sequence": "contig_1", "start": 718082, "stop": 718615, "strand": "+", "frame": 2, "gene": null, "product": "Potassium channel protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPR6", "UniRef:UniRef90_Q9PPR6" ], "nt": "ATGAAAGCATATCAACGCATGTTAGTGCCTGAAACTTTTTTCTCATTTTTTTATCAATTATTCGTCTTAATGATTGCTTTTTGTTCCGTTACAATTATTATTTATAATCAAATTGAATTAAAACAAGCTAATGAAGAGCACATAGACTTTATTGCTAAATATTTTGAAGTTTATTATGCTTTTAATATTTTTTTTATTATTGATTATTTAATTCGTATTTCATACGCTGATTACACATACAATTCAATTTCAAGACTAAAGGCTTTTTTAATAGCACCATTTCGTTTTGTTAATTTATTGGATCTAGTATCTTTTGTAGCAATTGTTATCATTTATAATACAGGATTATTAGATTTTGTAGCAAAAATGAATGAAACAACTTCATATTCACAAAAATTTGATTATTATTATCAAATCATCAATAATAATGCTTATATGAATGTTGTTCCTTGTATGTTTTTAATTAAAATCTTAGGTGCGCATTCTAAAATTATCAAAAATGTTATTTTGAGTAGTGAAGTTAATTTCTTTTGA", "aa": "MKAYQRMLVPETFFSFFYQLFVLMIAFCSVTIIIYNQIELKQANEEHIDFIAKYFEVYYAFNIFFIIDYLIRISYADYTYNSISRLKAFLIAPFRFVNLLDLVSFVAIVIIYNTGLLDFVAKMNETTSYSQKFDYYYQIINNNAYMNVVPCMFLIKILGAHSKIIKNVILSSEVNFF", "aa_hexdigest": "d72716abb700c472aaa7d2922dd4329e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 717992, "stop": 718918, "observations": { "mutation": [ 718073, 718613, 718634 ], "stop": [ 718073, 718613, 718634 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPR6", "query_cov": 1.0, "subject_cov": 0.4135514018691589, "identity": 0.8809999999999999, "score": 300.0, "evalue": 7.22e-99, "gene_start": 1, "gene_end": 177, "reference_start": 31, "reference_end": 207, "reference_sequence": "MTNDERKEFIKKNKAIYFNRIRYFFYEWTDMKAYQRMFVPETFFSFFYQLLVIMIAFCSVTIIIYNHVELKQANEAHLDFIAKYFEVYYAFNIFFIIDYLIRISYADYTYNSISRLKAFLIAPFRFVNLLDLVSFVAIIIIYNTGLLDLITKMNEATSINEKISCYYQIVNNNAYMNIVPCMFLIKILGAHSKIIKNIILSSEVNFFWQIFRRRWRVLLSSFLILVLFTFLLSFIIYRIEQNYYAINNILPGNPLYKHKNFGETLWFAMGTITTIAYGDITPVSPAGKVFAVILGVIGVTYYGFLTSLFASALISVINEYGKRRDNRRFLEFQSENERLLHEFSEQIKVDIVQELIKNNVIQDPEEIKRIENLQNQEREALKIKAKRRKKIYFSDDLFDDTKRISVSNVKVRSASNVTKVLGRSSSKK" }, "paralog": false, "identity": 0.8928571428571429, "score": 555.0, "evalue": 1.14e-201, "description": "Internal stop codon at 718073, 718613, 718634. Nonsense mutation around 718073, 718613, 718634.", "psc": { "uniref90_id": "UniRef90_Q9PPR6", "product": "Potassium channel protein", "uniref50_id": "UniRef50_Q9PPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPR6", "UniRef:UniRef50_Q9PPR6" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPR6" ], "product": "Potassium channel protein" } }, "id": "GPKLONFDEH_1229", "locus": "GCA000006625_01228" }, { "type": "cds", "sequence": "contig_1", "start": 718739, "stop": 719278, "strand": "+", "frame": 2, "gene": null, "product": "Potassium channel protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPR6", "UniRef:UniRef90_Q9PPR6" ], "nt": "TTGCCTGGTAATCCGTTGTACAAACTTAAAAATTTTGGTGAAACTTTGTGGTTTTCTATGGGAACAATTACCACGATAGCTTATGGTGATGTTACACCTGTTTCTCCAGCTGGTAAAGTATTTGCCGTTATTTTAGGTGTAATTGGTGTTACCTATTATGGTTTTCTGACTTCATTATTTGCTTCAGCTTTAATTTCAGTAATTAATGAATTTGGTAAACGCCGTGATAGCCGTAGATTGTTAGAATTTCAAAATGAAAACGAGCGATTATTGCATGAATTTTCTGAGCAAATTAAAGTAGATATTGTCCAAGAATTAATTAAAAATAATGTTATTCAGGATCCAGAAGAAATTAAACGAATTGAAGATCTTCAAAATCAAGAACGTGAAGCTTTAAAAATGAAAGCGAAACGCCGAAAGAAAATTTATTTTAGTGATGATTTATTTGATGATACAAAACGAATTAGTGTTTCAAACGTCAAAGTACGTAGTGCTTCAAATGTAACAAAAGTATTAGGTCGATCATCAACAAAAAAATAA", "aa": "MPGNPLYKLKNFGETLWFSMGTITTIAYGDVTPVSPAGKVFAVILGVIGVTYYGFLTSLFASALISVINEFGKRRDSRRLLEFQNENERLLHEFSEQIKVDIVQELIKNNVIQDPEEIKRIEDLQNQEREALKMKAKRRKKIYFSDDLFDDTKRISVSNVKVRSASNVTKVLGRSSTKK", "aa_hexdigest": "9430f8e1a47fbf8add4c5d4005dee0b6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 718439, "stop": 719278, "observations": { "mutation": [ 718613, 718634 ], "stop": [ 718613, 718634 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPR6", "query_cov": 1.0, "subject_cov": 0.4182242990654206, "identity": 0.9390000000000001, "score": 315.0, "evalue": 9.83e-105, "gene_start": 1, "gene_end": 179, "reference_start": 250, "reference_end": 428, "reference_sequence": "MTNDERKEFIKKNKAIYFNRIRYFFYEWTDMKAYQRMFVPETFFSFFYQLLVIMIAFCSVTIIIYNHVELKQANEAHLDFIAKYFEVYYAFNIFFIIDYLIRISYADYTYNSISRLKAFLIAPFRFVNLLDLVSFVAIIIIYNTGLLDLITKMNEATSINEKISCYYQIVNNNAYMNIVPCMFLIKILGAHSKIIKNIILSSEVNFFWQIFRRRWRVLLSSFLILVLFTFLLSFIIYRIEQNYYAINNILPGNPLYKHKNFGETLWFAMGTITTIAYGDITPVSPAGKVFAVILGVIGVTYYGFLTSLFASALISVINEYGKRRDNRRFLEFQSENERLLHEFSEQIKVDIVQELIKNNVIQDPEEIKRIENLQNQEREALKIKAKRRKKIYFSDDLFDDTKRISVSNVKVRSASNVTKVLGRSSSKK" }, "paralog": false, "identity": 0.899641577060932, "score": 495.0, "evalue": 8.98e-178, "description": "Internal stop codon at 718613, 718634. Nonsense mutation around 718613, 718634.", "psc": { "uniref90_id": "UniRef90_Q9PPR6", "product": "Potassium channel protein", "uniref50_id": "UniRef50_Q9PPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPR6", "UniRef:UniRef50_Q9PPR6" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPR6" ], "product": "Potassium channel protein" } }, "id": "GPKLONFDEH_1230", "locus": "GCA000006625_01229" }, { "type": "cds", "sequence": "contig_1", "start": 720003, "stop": 720125, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATACTCCGAATTTGCAAGACCCAAAAACTTATAATGAAAAACTACTTTATATGTATTTAAATACAGATCAATCGAAAATTTTAGAAATTGTCGATAAAATAAGTTTTAAAAAATGA", "aa": "MGYTPNLQDPKTYNEKLLYMYLNTDQSKILEIVDKISFKK", "aa_hexdigest": "99bd04080f1ed1cb38e57b825e787b65", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4784.5039, "isoelectric_point": 7.982813835144044 }, "id": "GPKLONFDEH_1231", "locus": "GCA000006625_01230" }, { "type": "cds", "sequence": "contig_1", "start": 720135, "stop": 720233, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACTGATAAATTAATTAAAGAATTAAATGAAATTACAAGATTATATTATAAAAATGATCGTAATCAATTTAAAAAATCTTTAATAAATATATGA", "aa": "MITDKLIKELNEITRLYYKNDRNQFKKSLINI", "aa_hexdigest": "db9393899d8223c0d0dac12865769362", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3956.6103000000003, "isoelectric_point": 9.600390052795408 }, "id": "GPKLONFDEH_1232", "locus": "GCA000006625_01231" }, { "type": "cds", "sequence": "contig_1", "start": 720347, "stop": 720574, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGTTAATTCTAATTCTGATTTGTTATTAAACACACAAACTAATCCTTATTTTATGGGATCACTTTTAATAAAAAAATCAATAAATAAAATAGTTGGATTTACATTAGTTTATACTCGTCCAAATATTGCAGAAATTCTTATTGAGCAAACAGATCGTGAAATTAGAGGTATGTATCAATATTTATTATCACAAAATTTAATAATTAATAATATTAATAATTGA", "aa": "MLVNSNSDLLLNTQTNPYFMGSLLIKKSINKIVGFTLVYTRPNIAEILIEQTDREIRGMYQYLLSQNLIINNINN", "aa_hexdigest": "956265830766ff660b4a2aaf89fbfad3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8647.950699999994, "isoelectric_point": 8.063592720031735 }, "id": "GPKLONFDEH_1233", "locus": "GCA000006625_01232" }, { "type": "cds", "sequence": "contig_1", "start": 720779, "stop": 720910, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTCCAATTGCTTTAGAAGCATTAAAAACAAATTTTAAAAAAGAATTTTTATTAGCAATTAATGGAATACAAGATGAAAATTATTTAAATAATTTATCTCAATTTAATTTTGAATTAATTTATAGTTGA", "aa": "MIPIALEALKTNFKKEFLLAINGIQDENYLNNLSQFNFELIYS", "aa_hexdigest": "f0d91f32b17a928bde30d37e1a1e2390", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5021.7370999999985, "isoelectric_point": 4.584314918518066 }, "id": "GPKLONFDEH_1234", "locus": "GCA000006625_01233" }, { "type": "cds", "sequence": "contig_1", "start": 721262, "stop": 721663, "strand": "-", "frame": 3, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627", "KEGG:K02996", "RefSeq:WP_006688496.1", "SO:0001217", "UniParc:UPI00001351DB", "UniRef:UniRef100_Q9PPR3", "UniRef:UniRef50_Q8R7Y9", "UniRef:UniRef90_Q9PPR3" ], "nt": "ATGCAAAAAAGTAATATTGTTGAATACAAAGGTTTAGGTCGTCGTAAATCTTCAATTGCAAGAGTTAAATTAGTTCCAGGTTCTGGAAAGGTTCTTGTTAATGATCGTCAACCAGAAAATTATTTTCCAAATAAATTAGTTATCCAAGACATGATGCAACCATTGGTTTTAACAAAAACAGCTGAAACTTATGATGTTTATGTTAAAGTTATTGGTGGTGGATTTAATGGACAAGCTGGAGCAATTCGTTTAGGAATTACTCGTGCTTTAATTCAAACTCGCGAAGATTTAAAAACCGATTTACGTAAAGCTGGTTTAGTAACACGTGATTCACGTGTTAAAGAACGTAAGAAATTTGGGCTATATGGTGCACGTCGTGCTCCTCAATTTACAAAACGTTAA", "aa": "MQKSNIVEYKGLGRRKSSIARVKLVPGSGKVLVNDRQPENYFPNKLVIQDMMQPLVLTKTAETYDVYVKVIGGGFNGQAGAIRLGITRALIQTREDLKTDLRKAGLVTRDSRVKERKKFGLYGARRAPQFTKR", "aa_hexdigest": "638aa3579e05ba8655ad30a37b265650", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00001351DB", "ncbi_nrp_id": "WP_006688496.1", "uniref100_id": "UniRef100_Q9PPR3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001351DB", "RefSeq:WP_006688496.1", "UniRef:UniRef100_Q9PPR3" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPR3", "uniref90_id": "UniRef90_Q9PPR3" }, "psc": { "uniref90_id": "UniRef90_Q9PPR3", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q8R7Y9", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q8R7Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8R7Y9" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "GPKLONFDEH_1235", "locus": "GCA000006625_01234" }, { "type": "cds", "sequence": "contig_1", "start": 721683, "stop": 721847, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGCAAATATTCAGATGAACTTATTCGTCGTTCAGTTAAAGGAATGCTTCCAAAAAATAAATTAGCAAAACAAATTCTAAACAAATTATTCATTTATAAAAATGATAAACATCCCCATGAAGCACAACAACCAACTATTTTGGAATTAAAATTAAAATAG", "aa": "MISKYSDELIRRSVKGMLPKNKLAKQILNKLFIYKNDKHPHEAQQPTILELKLK", "aa_hexdigest": "80fa0cefb490f4a33a84fd4059de7dc9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6389.581, "isoelectric_point": 9.997321891784669 }, "id": "GPKLONFDEH_1236", "locus": "GCA000006625_01235" }, { "type": "cds", "sequence": "contig_1", "start": 722443, "stop": 722637, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAGAATAAAATAATAACATCATCATTAGCTTTATTGTTTACAGGAGTAATAGCAACGATAAGTATTGCCGCTTGCTCAAAACAAAATAATCAAAAAACAAAAAATTTTAATTCACAAGAACAAGCAAATAAGATTATTGAGGCTATATTAAATAAAAAAGAAGGTAAGGATAATTTTATCGATTGA", "aa": "MKRKNKIITSSLALLFTGVIATISIAACSKQNNQKTKNFNSQEQANKIIEAILNKKEGKDNFID", "aa_hexdigest": "8dbfb4fe54a2568f58744c09e76010b4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7125.190599999999, "isoelectric_point": 9.845434379577636 }, "id": "GPKLONFDEH_1237", "locus": "GCA000006625_01236" }, { "type": "cds", "sequence": "contig_1", "start": 722734, "stop": 722838, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATAAAAATGAATATAAGTCAACTTTTAGTGCACAAATTGAAAATGTAATTAGGATCATTAAAGATGATATTAAAATGTATGCTAATTCACCATTTTGA", "aa": "MIDKNEYKSTFSAQIENVIRIIKDDIKMYANSPF", "aa_hexdigest": "95c4ffd2309b1de4357a211f45ec2d9f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4023.586800000001, "isoelectric_point": 6.000344657897949 }, "id": "GPKLONFDEH_1238", "locus": "GCA000006625_01237" }, { "type": "cds", "sequence": "contig_1", "start": 723244, "stop": 723339, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAACCAAAATTATAAAGGTTATTTAGCTCGTGAATTTTTAAAAATAAATCAAAAAAATGATTTAATTCCTTTACCAATGAGTATTGTTTGA", "aa": "MINQNYKGYLAREFLKINQKNDLIPLPMSIV", "aa_hexdigest": "3f0786630f4e02cd5089fe1566378f43", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3665.3290000000015, "isoelectric_point": 9.398732948303223 }, "id": "GPKLONFDEH_1239", "locus": "GCA000006625_01238" }, { "type": "cds", "sequence": "contig_1", "start": 723373, "stop": 723573, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATATACTTTATCAAAAGCTTTTGGAATGCCAAAAGATGAATTAGAACATTTGAAAGCACAAGAAAAATTTAAAATTCCAACTTTAAAAAAATTACTAACAAATGATGAATTAGAATTGAAAAATGGTATACAAACGATTAAAACAGGCATTGATTCGCCATTTAAATATCATCGCGATCAAAACCGTGATGATTGA", "aa": "MLYTLSKAFGMPKDELEHLKAQEKFKIPTLKKLLTNDELELKNGIQTIKTGIDSPFKYHRDQNRDD", "aa_hexdigest": "59fa1068d37813dc54dd5571b43ac6e7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7734.816099999997, "isoelectric_point": 7.986746406555176 }, "id": "GPKLONFDEH_1240", "locus": "GCA000006625_01239" }, { "type": "cds", "sequence": "contig_1", "start": 723619, "stop": 723768, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTAAAACCAGATTTATTAGTTAAAGGCGAACTATCAACTAGTGTCCATGAAGATCCTAACTTAGCTTATTATTTAAATGATGTAATAAAAAACCAATTAAGTGATATAGAACAAATTAGTATTAAAAAAAACCACATTAATTGA", "aa": "MGLKPDLLVKGELSTSVHEDPNLAYYLNDVIKNQLSDIEQISIKKNHIN", "aa_hexdigest": "3c502a750c01c94f61c18ce6699d151b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5549.268999999999, "isoelectric_point": 5.425247764587401 }, "id": "GPKLONFDEH_1241", "locus": "GCA000006625_01240" }, { "type": "cds", "sequence": "contig_1", "start": 723820, "stop": 723990, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTGCATGGCTTAATTAAAAATTCAGCTTTTAAAAAAATGATGGATGAAGGTAAACAAATTAAAAATTGGGCCTTTACTGACCGCAGATTTAGCGATGAAACACGCCAATATGTTAAAAAAGGTGAAGAAGATTTATATAGCCAAAACGCATCAATTCTTGGTTGA", "aa": "MGVHGLIKNSAFKKMMDEGKQIKNWAFTDRRFSDETRQYVKKGEEDLYSQNASILG", "aa_hexdigest": "8da63d32a9ac6e1b4a6abbca29aebad8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6498.299300000001, "isoelectric_point": 9.099341773986819 }, "id": "GPKLONFDEH_1242", "locus": "GCA000006625_01241" }, { "type": "cds", "sequence": "contig_1", "start": 724218, "stop": 724370, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTAGTGTTAAAAAAACAAAGCAAATAGGTTTGTTTACATCTATTGCAATTATGATTAGTAGCGTTGTGGGAATTGGTATTTTTTTTAAAAATGGATCTATTTTTCGGTTTAATAACTTTAATGAAATAGGAATTATTATTTCTTGA", "aa": "MKISVKKTKQIGLFTSIAIMISSVVGIGIFFKNGSIFRFNNFNEIGIIIS", "aa_hexdigest": "d02b8bfe4a7c48c4c31bf444b86e990b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5523.598699999997, "isoelectric_point": 10.582501411437985 }, "id": "GPKLONFDEH_1243", "locus": "GCA000006625_01242" }, { "type": "cds", "sequence": "contig_1", "start": 724371, "stop": 725189, "strand": "+", "frame": 3, "gene": "potE", "product": "APC family permease", "db_xrefs": [ "COG:COG0531", "COG:E", "SO:0001217", "UniRef:UniRef50_A0AAC9T1F8", "UniRef:UniRef90_A0AAC9T1F8" ], "nt": "GTGGTGGCAAGTTTAATCGCCTTTTTTACAGCATTAAGTTTTGCATATATTACCTTTTCTAAAAAATCTGGTTCTGGAATAGCTGGTATAATAGATGAATTAAAAGCTCCTAAATGTGCTCGTTTTATTTCTGTTTTGCAAACTTTTTTTTATAATGGAATACTGATGCCTTCCATTTCCTTTTTCGCTGCAGAATCATTATTAATGACAATAATACCTAAAAATTCATCATCACCACAAATATATCAAATTTTTATTTTGGCTATCGGGTTGTTTTTATTTTTTTTGTTGCTTAATTTTATTTCATTTAAATTTAGTTCAATTTTACAAAACATTGCAACAATTATTAAATTTATTCCTATTATTGCTATTGCTATTATTGGTATTACATATGGTATCAACAATGCACAAAATTCATTGTTCAATCATAATAATCCTAAGCATGCGAATTTTAGTCTTTTAGGAATATTAGCTTCTCTTCCTTCGATCTTATTCTCATTCGATTCTTTTTTAGGCATTTCATCATTACAACACAAAATTAAAAATGCTCAAAAAAATGTTCCAATTACATTAATTGTTGGTATGTTTTTAGTCGCTATTGTTTATATTCTAATAACTATAGGGCAAGTTTTTGTAGGCGAAGGTTATGCTTATGGTGTAATTAATAAAGTATTTAAAAATAATATCAATCTCCTAAAAATTATTACTATTATTGTTAATGTTTTTATTACAATTTCAATTATTGGTGTTTTAAATTCATTTGTTATTTTTTCAATTCATAATGCTCAATATGTAATTAATGAAAAAATTATTTTTTGA", "aa": "MVASLIAFFTALSFAYITFSKKSGSGIAGIIDELKAPKCARFISVLQTFFYNGILMPSISFFAAESLLMTIIPKNSSSPQIYQIFILAIGLFLFFLLLNFISFKFSSILQNIATIIKFIPIIAIAIIGITYGINNAQNSLFNHNNPKHANFSLLGILASLPSILFSFDSFLGISSLQHKIKNAQKNVPITLIVGMFLVAIVYILITIGQVFVGEGYAYGVINKVFKNNINLLKIITIIVNVFITISIIGVLNSFVIFSIHNAQYVINEKIIF", "aa_hexdigest": "89b0d22ace38cbb649d59c4e1f26c228", "start_type": "GTG", "rbs_motif": "4Base/6BMM", "genes": [ "potE" ], "truncated": "both", "pseudogene": { "start": 724218, "stop": 725492, "observations": { "mutation": [ 724368, 725187, 725190, 725196, 725271, 725475 ], "stop": [ 724368, 725187, 725190, 725196, 725271, 725475 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T1F8", "query_cov": 1.0, "subject_cov": 0.5065176908752328, "identity": 0.996, "score": 491.0, "evalue": 1.03e-170, "gene_start": 1, "gene_end": 272, "reference_start": 52, "reference_end": 323, "reference_sequence": "MKISVKKTKQIGLFTSIAIMISSVVGIGIFFKNGSIFRFNNFNEIGIIISWVVASLIAFFTALSFAYITFSKKSGSGIAGIIDELKAPKCARFISVLQTFFYNGILMPSISFFAAESLLMTIIPKNSSSPQIYQIFILAIGLFLFFLLLNFISFKFSSILQNIATIIKFIPIIAIAIIGITYGINNAQNSLFNHNNPKHANFSLLGILASLPSILFSFDSFLGISSLQHKIKNAQKNVPITLIVGMFLVAIVYILITIGQVFVGEGYAYGVINKVFKNNINLLKIITIIVNVFITISIIGVLNSFVIFSIHNAQYVINEKIIFWWSWIQRVQSKHFKEAQGLISVLFIFFTWITIFMIISIPLNTDAYIDLLSNYPIVFFFAVYGLIIAFAFYKKVKTKQKIKGIKKQYYTSDVTKRPKWFFNYQNSSLKNKNILNKELKIANNWILSVWIIGITSCLFVFLYQFLYGYTIYAWLDPYNLKLYTFGLFYANNVSVIPWIVSLLFFVMLIFFIGFPFLNDAILNLNHKKVNKQQKVFV" }, "paralog": false, "identity": 0.9858490566037735, "score": 789.0, "evalue": 1.11e-290, "description": "Internal stop codon at 724368, 725187, 725190, 725196, 725271, 725475. Nonsense mutation around 724368, 725187, 725190, 725196, 725271, 725475.", "psc": { "uniref90_id": "UniRef90_A0AAC9T1F8", "gene": "PotE", "product": "APC family permease", "uniref50_id": "UniRef50_A0AAC9T1F8", "cog_id": "COG0531", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T1F8", "UniRef:UniRef50_A0AAC9T1F8", "COG:COG0531", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T1F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T1F8" ], "product": "APC family permease" } }, "id": "GPKLONFDEH_1244", "locus": "GCA000006625_01243" }, { "type": "cds", "sequence": "contig_1", "start": 725286, "stop": 725477, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATTAGTATTCCATTAAATACAGACGCGTATATTGATTTATTATCTAATTATCCAATTGTTTTCTTTTTTGCAGTTTATGGTTTAATAATTGCTTTTGCCTTTTATAAAAAAGTTAAAACAAAGCAAAAAATAAAAGGCATTAAAAAACAATATTATACAAGTGATGTTACAAAACGTCCTAAATGA", "aa": "MIISIPLNTDAYIDLLSNYPIVFFFAVYGLIIAFAFYKKVKTKQKIKGIKKQYYTSDVTKRPK", "aa_hexdigest": "13c2585bf9d956af03058eb0a977b76d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7354.7341000000015, "isoelectric_point": 9.890175437927244 }, "id": "GPKLONFDEH_1245", "locus": "GCA000006625_01244" }, { "type": "cds", "sequence": "contig_1", "start": 726028, "stop": 726528, "strand": "+", "frame": 1, "gene": "secE", "product": "preprotein translocase subunit SecE", "db_xrefs": [ "COG:COG0690", "COG:U", "GO:0005886", "GO:0009306", "GO:0015450", "GO:0043952", "RefSeq:WP_006688437.1", "SO:0001217", "UniParc:UPI00000C1CCE", "UniRef:UniRef100_A0AAC9T2W1", "UniRef:UniRef50_A0AAC9T2W1", "UniRef:UniRef90_A0AAC9T2W1" ], "nt": "ATGGTTAATGACAAAAAACAAAAATCAAATAAATATTTAACGTACCAAGATCAAGAATATGAACGTAATCTTTTATTAGAAAAAGAACGTAAACAACAAAAAAAGATTTTGGCACAACAAGCATATCAACAATTAGATCTTAATAAAAAATTAATTAAAAAAGCTAAAAATGATCAAAAACTTATTTTAAGACTACTAAAAGAAGATAAATCAATTTCAAAAACACAATTTAATACCCACCGTTTAGAATTAAAAAATACAATTGATCAAATGGTTGATGAATATTATGAATTATTAGAACAATATAGTGTAGATTTTGAAAAAATAAGTTTTAAATTAAAACGTTGGCTCTATGGCATTAATAAAGAAATCAGACGTACTACTTGGGCTTCTAAACGTTCTGTTATTATCAGTTTAATCATTGTAATTGTTATTGTTTTAATTCTTGCTGCAATATTTTTTGGTATTGATACAGGTTTTTATAAATTATCAGCAAAATAA", "aa": "MVNDKKQKSNKYLTYQDQEYERNLLLEKERKQQKKILAQQAYQQLDLNKKLIKKAKNDQKLILRLLKEDKSISKTQFNTHRLELKNTIDQMVDEYYELLEQYSVDFEKISFKLKRWLYGINKEIRRTTWASKRSVIISLIIVIVIVLILAAIFFGIDTGFYKLSAK", "aa_hexdigest": "50e39382d02a9cb95353940a49210f12", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI00000C1CCE", "ncbi_nrp_id": "WP_006688437.1", "uniref100_id": "UniRef100_A0AAC9T2W1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CCE", "RefSeq:WP_006688437.1", "UniRef:UniRef100_A0AAC9T2W1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAC9T2W1", "uniref90_id": "UniRef90_A0AAC9T2W1" }, "psc": { "uniref90_id": "UniRef90_A0AAC9T2W1", "gene": "secE", "product": "preprotein translocase subunit SecE", "uniref50_id": "UniRef50_A0AAC9T2W1", "cog_id": "COG0690", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0009306", "GO:0015450", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2W1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2W1" ], "product": "Preprotein translocase subunit SecE" }, "genes": [ "secE" ], "id": "GPKLONFDEH_1246", "locus": "GCA000006625_01245" }, { "type": "cds", "sequence": "contig_1", "start": 726914, "stop": 727330, "strand": "+", "frame": 2, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "COG:COG0250", "COG:K", "GO:0006355", "KEGG:K02601", "SO:0001217", "UniRef:UniRef50_Q9PPQ8", "UniRef:UniRef90_Q9PPQ8" ], "nt": "ATGACCGATCAAATTTGGTTTTTAATTCGTAATACCCAAATGGTTACTGGTTTAGTTGGTTCATCTGGAAAAAATGTTAAACCAATTCCTGTACCTGAAGATAAGATCTTAAAATTAATTGCTGATAATGATGCTAAACGTGCTTTAATAAGTCTAAATGAAAAAGTTGACCATGAAGAAAATATTGAAGTTATTGAATTGCATGAAGACGAAAATTTTACGAACTTTGAAGTTGATCAAGAAGTTAAAATTATCGCAGATACTTTTTTTGGTGAAATTGCAAAAATTGCAAAAATTGATCAAAACAAGAAAATTGCCACTGTAGAATTTGAATTTTTTGGCCGAATTAACACACTAGATTTAAATTTTAATGATATTCAACTTTATGATGAAGAAAGTGATAAGTTAGAAAACTAA", "aa": "MTDQIWFLIRNTQMVTGLVGSSGKNVKPIPVPEDKILKLIADNDAKRALISLNEKVDHEENIEVIELHEDENFTNFEVDQEVKIIADTFFGEIAKIAKIDQNKKIATVEFEFFGRINTLDLNFNDIQLYDEESDKLEN", "aa_hexdigest": "7074678074fa8ec5a9cd83f603a7318c", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "5-prime", "pseudogene": { "start": 726614, "stop": 727330, "observations": { "mutation": [ 726629, 726818 ], "stop": [ 726629, 726818 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPQ8", "query_cov": 1.0, "subject_cov": 0.5328185328185329, "identity": 0.9570000000000001, "score": 256.0, "evalue": 2e-84, "gene_start": 1, "gene_end": 138, "reference_start": 121, "reference_end": 258, "reference_sequence": "MAYKIKDLDSKLLSDLKIDFNHKHQWYIVTVVSGNEQKVIENIKDKLNGYGYGDKLSDLKIIKEKIKEVKIYEPSEAPRSMKNRANTKWETIVVDGATKYRCTKIKEGNKFNGYIFLKAEMTDQIWFLIRNTQMVTGLVGSSGKNVKPIPVPEDKILKLIADNDAKRALISLNEKVNHEENIEVIKMHEDENFVNFEVGQEVKIIADTFFGEIAKIAKIDQNKKIATVEFEFFGRINTLDLNFNDIQLYDEENDKLENQ" }, "paralog": false, "identity": 0.9621848739495799, "score": 456.0, "evalue": 1.02e-165, "description": "Internal stop codon at 726629, 726818. Nonsense mutation around 726629, 726818.", "psc": { "uniref90_id": "UniRef90_Q9PPQ8", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_Q9PPQ8", "cog_id": "COG0250", "cog_category": "K", "kegg_orthology_id": "K02601", "go_ids": [ "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPQ8", "UniRef:UniRef50_Q9PPQ8", "COG:COG0250", "COG:K", "KEGG:K02601", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPQ8" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "GPKLONFDEH_1247", "locus": "GCA000006625_01246" }, { "type": "cds", "sequence": "contig_1", "start": 727817, "stop": 728101, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTATCAAAAAAAGGAATCGTCTTTCACCATAAATTCATTAAAGAAAATGAGTTATTTGATAAAGAATTTCTTTTTAAGAAAAAAAATCAATTTAAAGATTTAAAAACACAAAATGAAATTCAAAAAATAATCTCAACAACAAGTACAAAAATAAAACCAGGATATAAAAAGAAAATTAAAGAAGCTATTCAAAAAATTAATCAAAAAAACAAACGCTCTCATATCGAAAAGAAAATGAATGCTCACCGAATTCGTGGATATAAATTAAAAAATAGTTAA", "aa": "MNLSKKGIVFHHKFIKENELFDKEFLFKKKNQFKDLKTQNEIQKIISTTSTKIKPGYKKKIKEAIQKINQKNKRSHIEKKMNAHRIRGYKLKNS", "aa_hexdigest": "ef2ba9e03c2ad510cf2b8fc6af2cf88e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11301.25119999999, "isoelectric_point": 10.347127342224116 }, "id": "GPKLONFDEH_1248", "locus": "GCA000006625_01247" }, { "type": "cds", "sequence": "contig_1", "start": 728213, "stop": 728455, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37" ], "nt": "ATGAAGACTATTAAACCTTATTTATGTATCATTTTTGCAAATACAAAAAAAGAAGTTGAAGCAATTTATCAATATTTAATTCAACAAAATAAAAATGTTTGCATTTTGCATGGCTCACTACTTCAACGTGAACGAAAAAACAACTATCGTGATATTAAAAATAATAAATTTCAGTATGTTGTTGCCTCAGATATTGCAAGTCGTGGTTTAGATATTGATGGTGTAAGTCATATTATTAATTGA", "aa": "MKTIKPYLCIIFANTKKEVEAIYQYLIQQNKNVCILHGSLLQRERKNNYRDIKNNKFQYVVASDIASRGLDIDGVSHIIN", "aa_hexdigest": "0ff7dee521859e5dd86b957ed6d6a2c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 74, "aa_cov": 0.9125, "hmm_cov": 0.9864864864864865, "evalue": 1.6832733676706791e-18, "score": 62.52600860595703, "start": 8, "stop": 80 } ], "seq_stats": { "molecular_weight": 9313.700799999995, "isoelectric_point": 9.39209270477295 }, "id": "GPKLONFDEH_1249", "locus": "GCA000006625_01248" }, { "type": "cds", "sequence": "contig_1", "start": 728474, "stop": 729058, "strand": "-", "frame": 3, "gene": "srmB", "product": "ATP-dependent RNA helicase", "db_xrefs": [ "COG:COG0513", "COG:L", "SO:0001217", "UniRef:UniRef50_A0A9E2KV37", "UniRef:UniRef90_Q9PPQ7" ], "nt": "ATGCCATTAATCGCTAAAAGAGAAAATATTATTGGCGTTGCACCAACAGGGACAGGGAAAACTTTAGCCTTTGTTTTGCCTATATTAAACAACTTAGATTTATCTCAAAAACTGCAAGTTATAATTATTACTCCCACTCGTGAGTTAGCTCGCCAAATTTTTAGTAAAATTATTGTTTTTAAAAAACATCAACCACTTTTACAAGTTAAAATGTTAATTGGTGGTGAAAGTATTGACCAGCAAATTAATTCACAATTAAATAAAAGCCAACTGCTTATTGCAACTCCAACGCGTCTAAAACAAATTTTAACACGACAAATTCTTGATTTACACCATGTTAATGCACTTGTTTTTGATGAAGCTGATATGTTAATGGATCTTGGTTTTATTAGTGATTTAGAGTTTATTAACTCACAATTTACAAATAAAGATGTACAGAAAATAAGTTTTAGCGCTACACTGCACGAAATGTTATCGATCCAATTAAAAAAATATTTTAAAAATACAAAGATTATTAATATTAGTAATTCAATTTATGAAAATAAAAAAATAACCCATTATGTTATTCATAATCACGACAAATGA", "aa": "MPLIAKRENIIGVAPTGTGKTLAFVLPILNNLDLSQKLQVIIITPTRELARQIFSKIIVFKKHQPLLQVKMLIGGESIDQQINSQLNKSQLLIATPTRLKQILTRQILDLHHVNALVFDEADMLMDLGFISDLEFINSQFTNKDVQKISFSATLHEMLSIQLKKYFKNTKIINISNSIYENKKITHYVIHNHDK", "aa_hexdigest": "a7074c7a4c564365a804b81427c54d2d", "start_type": "ATG", "rbs_motif": null, "genes": [ "srmB" ], "truncated": "both", "pseudogene": { "start": 728171, "stop": 729148, "observations": { "mutation": [ 728191, 728215, 728476, 729121 ], "stop": [ 728191, 728215, 728476, 729121 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPQ7", "query_cov": 1.0, "subject_cov": 0.43792325056433407, "identity": 1.0, "score": 367.0, "evalue": 1.44e-124, "gene_start": 1, "gene_end": 194, "reference_start": 31, "reference_end": 224, "reference_sequence": "MKFNSNYQKWILDSLINQKIFEPTPIQLKTMPLIAKRENIIGVAPTGTGKTLAFVLPILNNLDLSQKLQVIIITPTRELARQIFSKIIVFKKHQPLLQVKMLIGGESIDQQINSQLNKSQLLIATPTRLKQILTRQILDLHHVNALVFDEADMLMDLGFISDLEFINSQFTNKDVQKISFSATLHEMLSIQLKKYFKNTKIINISNSIYENKKITHYVIHNHDKWHSLSIIMKTIKPYLCIIFANTKKEVEAIYQYLIQQNKNVCILHGSLLQRERKNNYRDIKNNKFQYVVASDIASRGLDIDGVSHIINWNLPTENEWYIHRAGRSGRGKYSGESYVLYDQKEHDKLLNLSKKGIVFHHKFIKENELFDKEFLFKKKNQFKDLKTQNEIQKIISTTSTKIKPGYKKKIKEAIQKINQKNKRSHIEKKMNAHRIRGYKLKNS" }, "paralog": false, "identity": 0.9876923076923076, "score": 622.0, "evalue": 1.29e-227, "description": "Internal stop codon at 728191, 728215, 728476, 729121. Nonsense mutation around 728191, 728215, 728476, 729121.", "psc": { "uniref90_id": "UniRef90_Q9PPQ7", "gene": "SrmB", "product": "ATP-dependent RNA helicase", "uniref50_id": "UniRef50_A0A9E2KV37", "cog_id": "COG0513", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPQ7", "UniRef:UniRef50_A0A9E2KV37", "COG:COG0513", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9E2KV37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2KV37" ], "product": "DEAD/DEAH box helicase" } }, "id": "GPKLONFDEH_1250", "locus": "GCA000006625_01249" }, { "type": "cds", "sequence": "contig_1", "start": 729536, "stop": 729865, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATACAAGATATAGTCAAGATCATCGTGATGCTATTTCAACTGTTGCTTTAATTATTCAATTTACTAACTTAATTTGGCCAGGTGCCGCATCAACAGTTGCTGTTTTAGTGTTAGCTAATTTAGGTGCTAACAAGCATGATTTAGCTAAGGAAAACACGCGCAAATTAATTAATTGGGGTATGATAGTTGGAATTAGTTTGGGAATTATTTTGTTAGTTTTATCATCGTTTGTAAATAAATTATTAAATCCACCGTTGGATTATACAGAACAAGGTTTAAAACATGCAGCATACACTGCGACAATTGCTATGTATTTAGAATGA", "aa": "MFYTRYSQDHRDAISTVALIIQFTNLIWPGAASTVAVLVLANLGANKHDLAKENTRKLINWGMIVGISLGIILLVLSSFVNKLLNPPLDYTEQGLKHAAYTATIAMYLE", "aa_hexdigest": "2dbc44f607e10c7c8c8c7967e7d903a8", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12046.94219999999, "isoelectric_point": 8.04489688873291 }, "id": "GPKLONFDEH_1251", "locus": "GCA000006625_01250" }, { "type": "cds", "sequence": "contig_1", "start": 729917, "stop": 730216, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCATTTTCATTAGCCACTGCTTATCGTCAACATAATAATGCAATCATGCCTTTAATTTCAAGTTCAATTGCAGTAACTGTTAATGTTATTTTAGATCCAATTTTGATTAAATATTGTGCCGTAACACCATTTGAGGCAATTTTATTTGTGGCTATTGCTACAATTGTTGCAAGATCTATTGATGCTTTAATAATGTTAATACTAACATTGTTTCGTAAACAATACCCTTATTATTTTTTAAATTATTTGAAATTAAGCAAAAAGGTCGTTAAATTAATTTTTATTCATGGTTGA", "aa": "MFSFSLATAYRQHNNAIMPLISSSIAVTVNVILDPILIKYCAVTPFEAILFVAIATIVARSIDALIMLILTLFRKQYPYYFLNYLKLSKKVVKLIFIHG", "aa_hexdigest": "6ef4b5f5595621ff1cdbfb3e228afd59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11227.48399999999, "isoelectric_point": 9.722493171691895 }, "id": "GPKLONFDEH_1252", "locus": "GCA000006625_01251" }, { "type": "cds", "sequence": "contig_1", "start": 730262, "stop": 730753, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCTTCGATTTTTTTAAAAAATCACAGAAAATAAAACCAAATTGGTTTATAAATCAATTTGGTGATAAAATTTTCATGAAAACAGCTGCTAAATTATTTTTCCCAGCAATTCTTGAAGTTATCATTTTATCGTCTGTTAATTATTTTGATAGTCTTTTCATTGCTTTATTTACACCTGAGAATATGGGGACAGCAGCCAAAACTGCAACCGTGTTAGCGACACAACTAATGTTTTTGCCAACTATTTTTTTCTATTCTATTGTAGCAGCAGCGGGGATTTTAGCCGCTCAATATTATGGTAAAAAAGACTATTTAAAATTTAAAGAAACAATTAACTTTATGTTGTTGTTTAGTTTTATTGTTGTAATGATATTTATTGTTATTTATATGGCAGCACCATTACAAATGATTCAATTATATTCAGGAAATAATTTAGATGTTTCAGCTTCAGAAGAAGCAAAACGAATTTATGATTTAACAAATCATTGA", "aa": "MRFDFFKKSQKIKPNWFINQFGDKIFMKTAAKLFFPAILEVIILSSVNYFDSLFIALFTPENMGTAAKTATVLATQLMFLPTIFFYSIVAAAGILAAQYYGKKDYLKFKETINFMLLFSFIVVMIFIVIYMAAPLQMIQLYSGNNLDVSASEEAKRIYDLTNH", "aa_hexdigest": "3a7fd3e2068eb2c358d45c8262c0dd3e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18688.958399999992, "isoelectric_point": 9.222411918640137 }, "id": "GPKLONFDEH_1253", "locus": "GCA000006625_01252" }, { "type": "cds", "sequence": "contig_1", "start": 731983, "stop": 732276, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTTACAATACGCAATGATTGGACAAGTTTTTTATGCTTTTTCATATCCTTTTGCAATTGCACACCGTCAAGATGCACGTCCAGCAATTCCTTTATTAGGAAGTATTGCCGCACTTTGTCTAAATTGTATTTTAGATCCAGTATTAATGATTTTAGTTGCCAAAACTCCTAAACAAGCGATATTACTTGCAGCATTCTCAACAATCGCTTCTCGTGGTTTTGATTTATTAATTGTTTTGCTTTGTGTTTGTATCTTTAAAAAAAGGGCAACATATGTTATTAATTATTGA", "aa": "MYLQYAMIGQVFYAFSYPFAIAHRQDARPAIPLLGSIAALCLNCILDPVLMILVAKTPKQAILLAAFSTIASRGFDLLIVLLCVCIFKKRATYVINY", "aa_hexdigest": "1de597eb850f6d65ab4f8edd5318f4ce", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10773.979799999985, "isoelectric_point": 9.236014747619631 }, "id": "GPKLONFDEH_1254", "locus": "GCA000006625_01253" }, { "type": "cds", "sequence": "contig_1", "start": 732382, "stop": 732789, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTATCAAATTGGTTTTAAACAAAAAAAATTGTCTTTTTTGAATTTTAAAAAAATATTTGGCGATAAACAATTTATCAAGAAAGCATTAAAATTATTTTTTCCAGCGATTATCCAAAATATTATCATTACAGCAATTGCTTATGTTGATAATTTTTTTCTAGCAACATATACCCCTCAATCACAAGGATTAGATGCTAAAACAGCAACAATTCTTGCAACTCAATTATTATATTTACCAACAATTTTTACCGTTGCTGTTTGTTATGCATGCAGTGTTTATAGCTCACAATATTTAGGAAATAAAAATTATCATAAATTTAAAGAAACAACAAATTACATGATTTTAGGATCTGTTATATTAAATATCCCTTTTTTAATTATTTATTTAACTTTTCCACATTGA", "aa": "MSYQIGFKQKKLSFLNFKKIFGDKQFIKKALKLFFPAIIQNIIITAIAYVDNFFLATYTPQSQGLDAKTATILATQLLYLPTIFTVAVCYACSVYSSQYLGNKNYHKFKETTNYMILGSVILNIPFLIIYLTFPH", "aa_hexdigest": "4f69db167290c3fb03b5b36b224e129b", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15477.16709999999, "isoelectric_point": 9.587367439270018 }, "id": "GPKLONFDEH_1255", "locus": "GCA000006625_01254" }, { "type": "cds", "sequence": "contig_1", "start": 733076, "stop": 733585, "strand": "-", "frame": 3, "gene": null, "product": "Deoxyribose-phosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7YYY5" ], "nt": "ATGAATAAGTATAATATTTATGTAGATCACACATTATTAAAAGCTGACGCAAGTTTAGAAGAGATTCATAAGTTGTGTGAAGAAGCAGAAGAAAATGAATTTTATTCAGTTTGTATTAATCCTTGTTTTGTTAAAGTTGCAAAACACTATTTATTAGAAACGTCTGTTAAAATTTGCACAGTTATTGGTTTTCCACTAGGCGCTAACACAATTGAAACTAAAGTTTTTGAAACAAAAAACGCTATAGATTTAGGTGCTGATGAAATTGATATGGTTATTAATATTAATCAATTAATCAATGGTAATAGAGAATATTGCTTAAATGAAATTAATCAAATTAAAGAAGTTTGTGGGGATAAAATTTTAAAAGTGATCGTTGAAACAGCATTTTTGAACAGGGAGCAAAAAGAATTTGCAGCTAAAATTATTCTAGAATCAAATGCTGATTTCATTAAAACATCAACTGGTTTTGCAAAAGAAGGTGCAAAACTTGAAGATATTATTCTATGA", "aa": "MNKYNIYVDHTLLKADASLEEIHKLCEEAEENEFYSVCINPCFVKVAKHYLLETSVKICTVIGFPLGANTIETKVFETKNAIDLGADEIDMVININQLINGNREYCLNEINQIKEVCGDKILKVIVETAFLNREQKEFAAKIILESNADFIKTSTGFAKEGAKLEDIIL", "aa_hexdigest": "f1ea2795a7cd8f375ed13cf93750f6b6", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A0M0LDG4", "query_cov": 0.9940828402366864, "subject_cov": 0.8, "identity": 0.506, "score": 165.0, "evalue": 1.33e-48, "valid": false, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_W7YYY5", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0009264" ] }, "pscc": { "uniref50_id": "UniRef50_W7YYY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7YYY5" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [], "id": "GPKLONFDEH_1256", "locus": "GCA000006625_01255" }, { "type": "cds", "sequence": "contig_1", "start": 733735, "stop": 734631, "strand": "-", "frame": 1, "gene": "tktA", "product": "transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "EC:2.2.1.1", "SO:0001217", "UniRef:UniRef50_A0A084EYA4", "UniRef:UniRef90_Q9PPQ3" ], "nt": "ATGCAAACTAATCCAGAACAAATGCAACGTTTATTAAATTATATTAATGGTAATTATGAAGATTTAAATAAATTATTAGATGAAAATAAAATTACTAATTTAAATGATTCTACTCGCAGTTATTTAAAACAATATTTTAATCAATTAAAAGATTTAAAATCAGCTCTTGTTTTATCTGCTGATTTAGCTAAATCAACTTTCACAAAAATAGGCGAAGGCGCTTTTAACGATAACCATAAAAATCCATATATTAAATTCGGTATTCGTGAATTTGCTATGGCAGGAGCGATGAATGGAATAAGTTTACATCAAGGCGTTAAAGCGATAGGAGGCACTTTTTTAGCTTTTTCTGATTATATGAAACCAGCAATTCGTTTAGCTGCCATTAGTAATTTAGCTAATTTATTTATTTTTAGTCACGATAGTTATGCAGTGGGTGGTGATGGACCTACACATCAACCTGTCGATCAATTACCAATGTTAAGAGCAATTCCAAATGTAGAAGTAATTCGTCCTGCTGATCATTATGAAGTTAAATATGCTCTTTCATATAGTTTTAAACAAAAACAAAAACCGATTTGTTTAATTACTTCACGTCAAACTATTAAACAAATTAATGATCAAAAACCACAAGATTTTAGTAAGGGAGCATATATTATTAATTCGCCTTTTTCTTTTTGTAAAGATGTAGATTACACGATTATTGCTAGTGGTAGTGAAGTTAGTTTAGCAAATGACGCAGCAAAAGAACTTTTTGAAAAACATAAACTTAAAATTAAAGTTATTTCTGCTTTTAACTTAAATTTATTTTTACGTCAAAAACCTGAAGATATTAAGAGCCTTTTAGCTTCAAAAAATGGTCTATTAGCAATTGAAGCATCAAGCGAAATGCTTTGA", "aa": "MQTNPEQMQRLLNYINGNYEDLNKLLDENKITNLNDSTRSYLKQYFNQLKDLKSALVLSADLAKSTFTKIGEGAFNDNHKNPYIKFGIREFAMAGAMNGISLHQGVKAIGGTFLAFSDYMKPAIRLAAISNLANLFIFSHDSYAVGGDGPTHQPVDQLPMLRAIPNVEVIRPADHYEVKYALSYSFKQKQKPICLITSRQTIKQINDQKPQDFSKGAYIINSPFSFCKDVDYTIIASGSEVSLANDAAKELFEKHKLKIKVISAFNLNLFLRQKPEDIKSLLASKNGLLAIEASSEML", "aa_hexdigest": "53b300d1a16cca878c730575d1fb3f64", "start_type": "ATG", "rbs_motif": null, "genes": [ "tktA" ], "truncated": "both", "pseudogene": { "start": 733597, "stop": 734931, "observations": { "mutation": [ 733734, 733737, 734655, 734883 ], "stop": [ 733734, 733737, 734655, 734883 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPQ3", "query_cov": 1.0, "subject_cov": 0.45565749235474007, "identity": 0.9129999999999999, "score": 534.0, "evalue": 1.43e-185, "gene_start": 1, "gene_end": 298, "reference_start": 310, "reference_end": 607, "reference_sequence": "MNRYVNAMRSLALQAINKANQGHSGMSISAAPIIYTLYKGLMTISKSHPKWFNRDRLVLSAGHGSMALYPVFYFSSLLTLDDIKNFRNDNHLTPGHPEVLSNNYIDASTGPLGQGVANAVGMAITESYLRAEFASLKGVVDHYTYCIVGDGDLQEGISYEAMSIAGKLKLSKLIILHDSNDYQLDSAVSDVNIEDLKMRVESMGWNYLKTDNNPENIFKAIAEAKWKKNVKPTFIEVKTIIGEGTSFENSNEAHAAAISKEELEKFGKRFHTKTNNFEFHQEIFDHFFFNVVARGESAYNQWQQLVDQYMQTNPEQMQRLLNYINGNYEDLNKMLDENKIVNLSDSTRSYLKQYFAQLKDLKSALVLSADLAKSTFTKIGENAFNDDYKNPYIKFGIREFAMAGAMNGISLHQGAKAIGGTFLAFSDYMKPAIRLTAISNLANLFIFSHDSYAVGGDGPTHQPVDQLPMLRAIPNVEVIRPADHYEVKHALSYSFKQKQKPICLVTSRQAIKQINEQKPQDFTKGAYIINSPFSFSENPDYTIIASGSEVSLANDAAKEIFEKHQLKVKVISAFNLNLFLQQKPEVIKNLVSSKNGLLAIEASSEMLWWKLSVYTDKFMQIAANQFGRSADGNKLMHEFGFSVENIINQLLNKK" }, "paralog": false, "identity": 0.9076576576576577, "score": 801.0, "evalue": 3e-293, "description": "Internal stop codon at 733734, 733737, 734655, 734883. Nonsense mutation around 733734, 733737, 734655, 734883.", "psc": { "uniref90_id": "UniRef90_Q9PPQ3", "gene": "TktA", "product": "transketolase", "ec_ids": [ "2.2.1.1" ], "uniref50_id": "UniRef50_A0A084EYA4", "cog_id": "COG0021", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPQ3", "EC:2.2.1.1", "UniRef:UniRef50_A0A084EYA4", "COG:COG0021", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_A0A084EYA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A084EYA4" ], "product": "Transketolase" } }, "id": "GPKLONFDEH_1257", "locus": "GCA000006625_01256" }, { "type": "cds", "sequence": "contig_1", "start": 734944, "stop": 735390, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "TTGGTTTTATCAGCTGGTCATGGATCGATGGCATTATATCCTGTTTTTTATTTTTCATCATTATTAACATTAGATGACATTAAAAATTTTCGCAATGATAATTATTTAACACCAGGTCATCCTGAAGTGTTGGCTAATAATTATATTGATGCATCAACAGGACCACTAGGGCAAGGGGTTGCTAATGCAGTTGGTATGGCAATTACTGAAAGTTATTTAAGAACTGAATTTGCTACTCTTAAAGGTGTTATTGATCATTATACTTATTGTATTGTTGGCGATGGGGACCTACAAGAGGGAATTTGTTATGAGGCAATGAGTATTGCTGGAAAATTAAAATTATCGAAATTAATTATTTTACATGATTCAAATGATTACCAATTAGATTCAGCAGTAAGTGATGTTAATATTGAAGATTTAAAAATGCGTGTTGAATCAATGGGTTGA", "aa": "MVLSAGHGSMALYPVFYFSSLLTLDDIKNFRNDNYLTPGHPEVLANNYIDASTGPLGQGVANAVGMAITESYLRTEFATLKGVIDHYTYCIVGDGDLQEGICYEAMSIAGKLKLSKLIILHDSNDYQLDSAVSDVNIEDLKMRVESMG", "aa_hexdigest": "2b7b40f0304056455a264faddd5146cb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 148, "aa_cov": 0.9864864864864865, "hmm_cov": 1.0, "evalue": 2.3597888447637776e-47, "score": 157.1142578125, "start": 2, "stop": 147 } ], "seq_stats": { "molecular_weight": 16148.13909999999, "isoelectric_point": 4.542254066467286 }, "id": "GPKLONFDEH_1258", "locus": "GCA000006625_01257" }, { "type": "cds", "sequence": "contig_1", "start": 735406, "stop": 735558, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "ATGAATCGTTATGTTAACGCAATGCGTTCACTTGCTTTACAGGCTATTAATAAAGCAAAGCAAGGTCACAGTGGAATGAGTATTTCAGCTGCACCTATTGTTTATACCCTTTATAAGGGATTAATGACTATTTCAAAATCACATCCAAAGTGA", "aa": "MNRYVNAMRSLALQAINKAKQGHSGMSISAAPIVYTLYKGLMTISKSHPK", "aa_hexdigest": "45d8c5fe165557a647d5ccec723ef389", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 45, "aa_cov": 0.9, "hmm_cov": 1.0, "evalue": 2.369198545204752e-09, "score": 32.19496536254883, "start": 2, "stop": 46 } ], "seq_stats": { "molecular_weight": 5478.4427, "isoelectric_point": 10.461107444763183 }, "id": "GPKLONFDEH_1259", "locus": "GCA000006625_01258" }, { "type": "cds", "sequence": "contig_1", "start": 735588, "stop": 735851, "strand": "-", "frame": 2, "gene": "ptsH", "product": "Phosphocarrier protein HPr", "db_xrefs": [ "COG:COG1925", "COG:TG", "RefSeq:WP_006688423.1", "SO:0001217", "UniParc:UPI00000C1CD4", "UniRef:UniRef100_Q9PPQ2", "UniRef:UniRef50_Q9PPQ2", "UniRef:UniRef90_Q9PPQ2" ], "nt": "ATGATATCGAAAGAATTTACCATCGTAGCAGAATTGTCAGCATTTATTAAGGCGATCACTTATTTTGTTAATGCAGCTAGTGATTTTAGCTCTTCAATTACTATTAAAGCAAATGGGCGTCAAGCAGATGCTAAATCAATTATCAACATTATGGCTTTAGGAATTAAACAAGGCACAAAAATCGAGCTGAGTGCCGTTGGAAATGATGCAAATAAAGCAATTAACAAATTAGAAGAAATTTTAATTGAACAGAAATTAATTTAA", "aa": "MISKEFTIVAELSAFIKAITYFVNAASDFSSSITIKANGRQADAKSIINIMALGIKQGTKIELSAVGNDANKAINKLEEILIEQKLI", "aa_hexdigest": "453cc491f882c2d70f0befdeb2de86ca", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000C1CD4", "ncbi_nrp_id": "WP_006688423.1", "uniref100_id": "UniRef100_Q9PPQ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C1CD4", "RefSeq:WP_006688423.1", "UniRef:UniRef100_Q9PPQ2" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPQ2", "uniref90_id": "UniRef90_Q9PPQ2" }, "psc": { "uniref90_id": "UniRef90_Q9PPQ2", "gene": "PtsH", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_Q9PPQ2", "cog_id": "COG1925", "cog_category": "TG" }, "pscc": { "uniref50_id": "UniRef50_Q9PPQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPQ2" ], "product": "Phosphocarrier protein HPr" }, "genes": [ "ptsH" ], "id": "GPKLONFDEH_1260", "locus": "GCA000006625_01259" }, { "type": "cds", "sequence": "contig_1", "start": 735951, "stop": 736274, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13091.12" ], "nt": "ATGAATCGTTTAAGTTATGAATCATTAATTAAGTCCGGTGTACAAATTTATGAATATAGTGGTTTTTTACACACTAAAGCGATTTTAATAGATGATGATATTTCACTATTAGGTTCTTATAATCTTGATAATAGAGCATTAGTAATTAATTACGAATCATTATTAACAATTTATTCAATACAAGTAAATCACGACATTAACGAGATTTTTAATCAATATCTCCGTAATTCACAGTTAATTAATTTAGAAGATTTAGATAACAAATTCACATTCAACGAATTAGTTCAAACAGTATTTATTAATATTTTTCAATTACTTTTATAA", "aa": "MNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLLTIYSIQVNHDINEIFNQYLRNSQLINLEDLDNKFTFNELVQTVFINIFQLLL", "aa_hexdigest": "9dd3e8b9294ffea2edef682a9f8d8094", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13091.12", "name": "PLDc_2", "length": 72, "aa_cov": 0.6448598130841121, "hmm_cov": 1.0, "evalue": 4.108313643322171e-18, "score": 61.104087829589844, "start": 5, "stop": 73 } ], "seq_stats": { "molecular_weight": 12525.082899999987, "isoelectric_point": 4.456313514709472 }, "id": "GPKLONFDEH_1261", "locus": "GCA000006625_01260" }, { "type": "cds", "sequence": "contig_1", "start": 736290, "stop": 736565, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13091.12" ], "nt": "ATGCATCCTCAACCTTACTTATGTCAAGGAAAAGATGACTTAATAAATAATTCTATTAAAAATAAAACAATAACAACACTTTTGCATACAGGACCAGACCAATCAACAAGTTCTTTAAATGAAATTCTGGTTATGGCAATCGCTAATGCTAAAAAATCAATTACAATTTTTACGCCATATTTATTTCCTACTGAAGATATTTTAATAGGACTTAAAGCTGCAGCGAATACTGGAATAAAAGTTAAAATTATTTTACCTGGAAGGGTTGATTCTTGA", "aa": "MHPQPYLCQGKDDLINNSIKNKTITTLLHTGPDQSTSSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLKAAANTGIKVKIILPGRVDS", "aa_hexdigest": "1bf27ebb30228a55fd74795d162bb131", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13091.12", "name": "PLDc_2", "length": 44, "aa_cov": 0.4835164835164835, "hmm_cov": 1.0, "evalue": 4.474793841234322e-10, "score": 35.099185943603516, "start": 44, "stop": 87 } ], "seq_stats": { "molecular_weight": 9876.429299999989, "isoelectric_point": 8.819871330261233 }, "id": "GPKLONFDEH_1262", "locus": "GCA000006625_01261" }, { "type": "cds", "sequence": "contig_1", "start": 736983, "stop": 737315, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAATTATATTCTTTTGGATTTTTGGATCACGCCCATTTAAAGATAAAAAAATTAATGATATCATTACAAACCGTCGTGCACTTTTTAAATTTGAAGATTATGAATTTTATCATAGTTTTCAAAATATGATTATTAATAATAATGGACTTAATTTAATTCGTAAAATTGGTCAGTATAATTACCAACTTTTTCGTACACCTATTTATTGTGATAATCAAATAACAATTATTAAAGAGACAAACGAAATGTATGAACAAACTATTAAATTAATAAGAAAAGCAAAAAAAACAATTTATTTACAATATTATATTATTGAGAATGGTCGTTGA", "aa": "MSIIFFWIFGSRPFKDKKINDIITNRRALFKFEDYEFYHSFQNMIINNNGLNLIRKIGQYNYQLFRTPIYCDNQITIIKETNEMYEQTIKLIRKAKKTIYLQYYIIENGR", "aa_hexdigest": "efc83defcf7d662f53bc31e19dacca7a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13526.565399999983, "isoelectric_point": 9.574667167663574 }, "id": "GPKLONFDEH_1263", "locus": "GCA000006625_01262" }, { "type": "cds", "sequence": "contig_1", "start": 737343, "stop": 737549, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACGTATTTTAGCCAAATTCATCATAACATTTATTTGTGTATTATTAATAATTAATATTTGTTTAATATCAATATATTTTCACCATCGACCAACGGTTATTGTTACAGATATTTTAATATCATTTTATATTTTAAATACTAGTATTGGCTTATATATTTTATCTTCAAGACATCGGCAAGATCGTTCTAAAAAATCATGA", "aa": "MKKRILAKFIITFICVLLIINICLISIYFHHRPTVIVTDILISFYILNTSIGLYILSSRHRQDRSKKS", "aa_hexdigest": "b226293d2fb5740e67d5062b02735baa", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7992.647899999995, "isoelectric_point": 10.228569984436035 }, "id": "GPKLONFDEH_1264", "locus": "GCA000006625_01263" }, { "type": "tRNA", "sequence": "contig_1", "start": 737628, "stop": 737716, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 78.5, "nt": "GGAAACATACTCAAGTGGTTAAGAGGGCACCCTGCTAAGGTGTTAGGTCGTTCTGCGGCGCGTGGGTTCGAATCCCACTGTTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 737681, 737683 ], "id": "GPKLONFDEH_11", "locus": "GCA000006625_01264" }, { "type": "cds", "sequence": "contig_1", "start": 737806, "stop": 738018, "strand": "-", "frame": 1, "gene": "trxA", "product": "thioredoxin", "db_xrefs": [ "COG:COG3118", "COG:O", "GO:0015035", "SO:0001217", "UniRef:UniRef50_Q9PPQ0", "UniRef:UniRef90_Q9PPQ0" ], "nt": "ATGCTTATTCCAGTATTAGATTCAATTGAAAAAAAACATGGTGATGAATTTACTATTATTAAAATAAATGTTGATCATTTTCCAGAATTATCAACACAATATCAAGTCAAATCAATCCCTGCTTTATTTTACTTAAAAAATGGAGATATTAAAGCGACAAGTTTAGGATTTATTGATGAAAATTCTTTAGTTAATAAATTACGATCAATTTAA", "aa": "MLIPVLDSIEKKHGDEFTIIKINVDHFPELSTQYQVKSIPALFYLKNGDIKATSLGFIDENSLVNKLRSI", "aa_hexdigest": "22d1e93dd80a069156da51cb1e62d52e", "start_type": "ATG", "rbs_motif": null, "genes": [ "trxA" ], "truncated": "3-prime", "pseudogene": { "start": 737806, "stop": 738126, "observations": { "mutation": [ 738036 ], "stop": [ 738036 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPQ0", "query_cov": 1.0, "subject_cov": 0.660377358490566, "identity": 1.0, "score": 140.0, "evalue": 4.17e-42, "gene_start": 1, "gene_end": 70, "reference_start": 37, "reference_end": 106, "reference_sequence": "MLIKLESNQNLNELLKENHSKPILIDFYADWCPPCRMLIPVLDSIEKKHGDEFTIIKINVDHFPELSTQYQVKSIPALFYLKNGDIKATSLGFIDENSLVNKLRSI" }, "paralog": false, "identity": 0.9905660377358491, "score": 211.0, "evalue": 1.44e-72, "description": "Internal stop codon at 738036. Nonsense mutation around 738036.", "psc": { "uniref90_id": "UniRef90_Q9PPQ0", "gene": "trxA", "product": "thioredoxin", "uniref50_id": "UniRef50_Q9PPQ0", "cog_id": "COG3118", "cog_category": "O", "go_ids": [ "GO:0015035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPQ0", "UniRef:UniRef50_Q9PPQ0", "COG:COG3118", "COG:O", "GO:0015035" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPQ0" ], "product": "Thioredoxin" } }, "id": "GPKLONFDEH_1265", "locus": "GCA000006625_01265" }, { "type": "cds", "sequence": "contig_1", "start": 738034, "stop": 738126, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTAAATTAGAAAGTAACCAAAATTTAAATGAATTATTAAAGGAAAATCATTCTAAACCAATATTAATTGATTTTTATGCGGATTGA", "aa": "MLIKLESNQNLNELLKENHSKPILIDFYAD", "aa_hexdigest": "fe0110f0c5f1e3eff85c0f822d21b168", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3544.0370000000003, "isoelectric_point": 5.0082201004028315 }, "id": "GPKLONFDEH_1266", "locus": "GCA000006625_01266" }, { "type": "cds", "sequence": "contig_1", "start": 738228, "stop": 738410, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATCCAATAAAATATTTATTTTTACGATTTTTACGTTTAACCAATGATTTTGAACAGTTAGGACATAATTCGTTAAGAATTTCTGGTTTCTCTAACGATTCTGTAAAACGACATGTAGGGAAATTTGAACACGCAATGAACCGAGTGTTCATTCTAGATCTTTTAATCAATAAATCATGA", "aa": "MHPIKYLFLRFLRLTNDFEQLGHNSLRISGFSNDSVKRHVGKFEHAMNRVFILDLLINKS", "aa_hexdigest": "2a251e1a30ebd6925505ef42853840df", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7115.209799999999, "isoelectric_point": 10.151272392272947 }, "id": "GPKLONFDEH_1267", "locus": "GCA000006625_01267" }, { "type": "cds", "sequence": "contig_1", "start": 738524, "stop": 738706, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACTATTGGCACCACTAGGGGTTACATCAATAAAGATAAGAGTAAATTAATTCCAACAGAATTAGGGATTAAAGTCATCCAAGAGTTAAAAGAAGCCTTTCCAAAAGTGATTTCTATTAAATTTACATCTGAGATGGAAGAACGTTTAGATGTTATAGCTAATGGCAAATTATTTTGA", "aa": "MATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLDVIANGKLF", "aa_hexdigest": "6271983b6bb0a7e320acfb890dc9b8ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6754.907399999998, "isoelectric_point": 9.099599647521973 }, "id": "GPKLONFDEH_1268", "locus": "GCA000006625_01268" }, { "type": "cds", "sequence": "contig_1", "start": 738902, "stop": 739000, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTGCCAACTTTTAATAAGCAAGTAATTCGTTTTATTAATAATGAATATAAATTTTATACAGCTTATAAAACAATTCGAACTTTAGGATATTGA", "aa": "MKVPTFNKQVIRFINNEYKFYTAYKTIRTLGY", "aa_hexdigest": "213444f41ed683ceb3167793a0a392b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3948.589500000001, "isoelectric_point": 9.9959680557251 }, "id": "GPKLONFDEH_1269", "locus": "GCA000006625_01269" }, { "type": "cds", "sequence": "contig_1", "start": 739022, "stop": 739309, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAGGATTAGAAATTAATAATCAGCAATTAGCTTTAATAACTTATCCAAGAACAGATAGCGAGCGATTAAGTGATGATTTTTTACAAGAAGCTAATGCCTTTATTGAAAATAATTTTGGTAAAGAATATCTAGAAAATTTTAAAAATAAAAAAATTAATAGAAAAGAAGTAAATATTCAAGACGCACATGAAGCAATTCGCCCTGTTGATATTAATTTAAAACCTGAAGAAATTAAAGATTATGTTTCATTAGATATTTATAAGCTGTATAACTTAGTTTGA", "aa": "MFEGLEINNQQLALITYPRTDSERLSDDFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPVDINLKPEEIKDYVSLDIYKLYNLV", "aa_hexdigest": "51608af05319ad1fd7ea31989540cf77", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11252.513799999988, "isoelectric_point": 4.729879570007324 }, "id": "GPKLONFDEH_1270", "locus": "GCA000006625_01270" }, { "type": "cds", "sequence": "contig_1", "start": 739343, "stop": 739471, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGATGATTTAAGTGATATTTTTAAAGTTTATAGTATTGATGATTTGAAAATCACCAAAGGCGATGTTCAATTACCATTAACAACTGATAAATTATTACAAATCGCATCCAATAATTTTGGTTGA", "aa": "MIDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFG", "aa_hexdigest": "82d4722100f64144a16398f96b305bda", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4699.3315999999995, "isoelectric_point": 4.219522285461426 }, "id": "GPKLONFDEH_1271", "locus": "GCA000006625_01271" }, { "type": "cds", "sequence": "contig_1", "start": 739607, "stop": 739780, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGATTCGCAATTTGCAAGACGAATTTTAGATCGAGTTATTGGTTATAAACTTTCTGGATTAGTTAAAAAAAACTTACATGGCTTATCTGCTGGGCGAGTACAATCGATCGCTTTATTATTTGTAGTTGAGAGAGAGTTAGAAAGACGTTCTTTTGTTAAAGAAGAATGA", "aa": "MVDSQFARRILDRVIGYKLSGLVKKNLHGLSAGRVQSIALLFVVERELERRSFVKEE", "aa_hexdigest": "6dc441dd1fd88b7668d5e0a64d06611a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6558.616099999999, "isoelectric_point": 10.141988945007324 }, "id": "GPKLONFDEH_1272", "locus": "GCA000006625_01272" }, { "type": "cds", "sequence": "contig_1", "start": 740033, "stop": 740188, "strand": "-", "frame": 3, "gene": null, "product": "toprim domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A54A148" ], "nt": "ATGCAAAAAGATTTAATAGTAGTAGAGTCACCAAATAAAATTAAATCAATTCAGTCTTATTTAGGTGATAGTTATAATGTAATAGCTACATGTGGACATTTACGTGAGCTCGCAAAAAAATCTGGTTATGATCCAAAAACTTATGATTTAAAATGA", "aa": "MQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLK", "aa_hexdigest": "dbfadc68afcddc005a4213e31fd1059e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI000A54A148", "query_cov": 0.9411764705882353, "subject_cov": 0.8275862068965517, "identity": 0.7709999999999999, "score": 80.9, "evalue": 3.05e-19, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_UPI000A54A148" }, "pscc": { "uniref50_id": "UniRef50_UPI000A54A148", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A54A148" ], "product": "toprim domain-containing protein" }, "genes": [], "id": "GPKLONFDEH_1273", "locus": "GCA000006625_01273" }, { "type": "cds", "sequence": "contig_1", "start": 740263, "stop": 740682, "strand": "-", "frame": 1, "gene": "smf", "product": "Unique hypothetical", "db_xrefs": [ "COG:COG0758", "COG:L", "SO:0001217", "UniRef:UniRef50_Q9PPP8", "UniRef:UniRef90_Q9PPP8" ], "nt": "TTGATAAAAGCAGGATATGGATTTGTTATTTATTATGATAGTGTTGTTTTGGACACACTTATCAATCGTTTAATTTCTCTTAAAGCTAGAATATGTATCATCACAGATGAGGGAATCTATAGTTCTAAAAACATTAAATACTTAAGTTACTGCAATGTTAGTGTAATATCTGAAATTCCAAAAGATTACAATAGTAATAAAATTACACAGGATTTAAAACGTCTTGCATGCGCTTCAAGCGACCATATTTTATGTGTTAATGAGTTATATCAAATAGATGATTATCTTTTTAATAAATATAAAGATTGCCAATTAGATTTGGTGTTATTAGATAAAAAATTAGCTAAGCAAAACACAATTTATCCCAAATACATTAATCGTATTTATCAATTTATTAAAAATTCAAAATTAATAAATTAA", "aa": "MIKAGYGFVIYYDSVVLDTLINRLISLKARICIITDEGIYSSKNIKYLSYCNVSVISEIPKDYNSNKITQDLKRLACASSDHILCVNELYQIDDYLFNKYKDCQLDLVLLDKKLAKQNTIYPKYINRIYQFIKNSKLIN", "aa_hexdigest": "b5006b1b8bd604b3b8772e2041ee4285", "start_type": "TTG", "rbs_motif": null, "genes": [ "smf" ], "truncated": "3-prime", "pseudogene": { "start": 740263, "stop": 740982, "observations": { "mutation": [ 740751, 740928 ], "stop": [ 740751, 740928 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPP8", "query_cov": 1.0, "subject_cov": 0.5815899581589958, "identity": 0.993, "score": 277.0, "evalue": 4.61e-93, "gene_start": 1, "gene_end": 139, "reference_start": 101, "reference_end": 239, "reference_sequence": "MIPLNYLTHYFSLKYKGDWSKIYLALINHEDQNEYLFLEKEDYEKVKAIDLTDEKYPNKYKKINQPPFILYYSTTVEWLDKKNIWLIDYENAISNYDILTLIKAGYGFVIYYDSVVLDTLINRLISLKARICIITDEGIYSSKNIKYLSYCNVSVISEIPKDYNSNKITQDLKRLACASSDHILCVNELYQIDDYLFNKYKDCQLDLVLLDKKLAKQNTIYPKYINRIYQFIKNSKLIN" }, "paralog": false, "identity": 0.9874476987447699, "score": 477.0, "evalue": 2.16e-174, "description": "Internal stop codon at 740751, 740928. Nonsense mutation around 740751, 740928.", "psc": { "uniref90_id": "UniRef90_Q9PPP8", "gene": "Smf", "product": "Unique hypothetical", "uniref50_id": "UniRef50_Q9PPP8", "cog_id": "COG0758", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPP8", "UniRef:UniRef50_Q9PPP8", "COG:COG0758", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPP8" ], "product": "Unique hypothetical" } }, "id": "GPKLONFDEH_1274", "locus": "GCA000006625_01274" }, { "type": "cds", "sequence": "contig_1", "start": 741024, "stop": 741488, "strand": "-", "frame": 2, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_A0AAP9D7M6", "UniRef:UniRef90_Q9PPP7" ], "nt": "ATGATTATTGGTCTACCAAATATTGGTAAGTCTTCTTTGATTAATTTTTTAGCTCCCAAAAAAGTATTAAAAGTTGAAAATAGACCTGGAGTTACTAAGACTCAATGTATTCGTCAAATCAATCAACATTTTTATTTAATTGATACACCTGGAATTTTTTTAAAAAATATTCAAAATGAGCGTGATAGTTTTGTTTTGACTTTGATTAATTGTGTTAAGAAAGAAGTTTTGGAATTAGAATCAATTGTACATTTTGCATATGATTTTTATTTAAAAAATTATAGGAATGAATTATTTATAAAATATAAAATGAATAAGGTATTAAATTTTGATGATTTTATTCATCATATTTGTGAGTTATATAATTATAAATTAACAAATAATGAATTTGATCATGTAAGAGCTTATGAGAATTTATTTAATGATTTTTCTAATGGATTGATTTGTAAAGTTAATTATGATTAG", "aa": "MIIGLPNIGKSSLINFLAPKKVLKVENRPGVTKTQCIRQINQHFYLIDTPGIFLKNIQNERDSFVLTLINCVKKEVLELESIVHFAYDFYLKNYRNELFIKYKMNKVLNFDDFIHHICELYNYKLTNNEFDHVRAYENLFNDFSNGLICKVNYD", "aa_hexdigest": "3dc1376187c3eb336ce41b9d4ce99dd8", "start_type": "ATG", "rbs_motif": null, "genes": [ "ylqF" ], "truncated": "3-prime", "pseudogene": { "start": 741024, "stop": 741788, "observations": { "mutation": [ 741638 ], "stop": [ 741638 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPP7", "query_cov": 1.0, "subject_cov": 0.5641025641025641, "identity": 1.0, "score": 314.0, "evalue": 6.6e-107, "gene_start": 1, "gene_end": 154, "reference_start": 120, "reference_end": 273, "reference_sequence": "MDIINKKINWFPGHMKKATDEILKNLKNVDFFIQLVDARCPLTSSNIELLKEVSSKPVINLANKADLSDWKKNFDNNFLLISTKKRNDKNKIIQHLYQLFDQKIKIYQKKGLINPKFVGMIIGLPNIGKSSLINFLAPKKVLKVENRPGVTKTQCIRQINQHFYLIDTPGIFLKNIQNERDSFVLTLINCVKKEVLELESIVHFAYDFYLKNYRNELFIKYKMNKVLNFDDFIHHICELYNYKLTNNEFDHVRAYENLFNDFSNGLICKVNYD" }, "paralog": false, "identity": 0.9960629921259843, "score": 512.0, "evalue": 2.64e-187, "description": "Internal stop codon at 741638. Nonsense mutation around 741638.", "psc": { "uniref90_id": "UniRef90_Q9PPP7", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_A0AAP9D7M6", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPP7", "UniRef:UniRef50_A0AAP9D7M6", "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAP9D7M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP9D7M6" ], "product": "Ribosome biogenesis GTPase A" } }, "id": "GPKLONFDEH_1275", "locus": "GCA000006625_01275" }, { "type": "cds", "sequence": "contig_1", "start": 741636, "stop": 741803, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGCAACAGATGAGATTTTAAAAAATCTTAAAAATGTTGATTTTTTTATTCAATTAGTTGATGCTAGATGTCCATTAACATCAAGTAATATTGAATTATTAAAAGAAGTTTCATCCAAACCAGTTATTAATTTGGCTAATAAGGCCGATTTAAGTGATTGA", "aa": "MKKATDEILKNLKNVDFFIQLVDARCPLTSSNIELLKEVSSKPVINLANKADLSD", "aa_hexdigest": "cff49dbbc4105b08a8b2bd5e21dfe2b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6132.066299999999, "isoelectric_point": 6.143294715881347 }, "id": "GPKLONFDEH_1276", "locus": "GCA000006625_01276" }, { "type": "cds", "sequence": "contig_1", "start": 741857, "stop": 742081, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B4", "UniRef:UniRef90_A0AAC9T0B4" ], "nt": "ATGTTATTTATTACAATAATTTATCTTTTATGGATCAATTGTCATAATAGCACAGCAAGATATTTAATTTTAAAAATCAATAAATACCCACCGAAAAAACCGATCAAAAATCTTCCTTTTATTTTTTTTAGATTAGCTTATTATACATTTAATATAAGACAAAAAATTCAATATCAACAAAAACAAATTTATAAATATTTAATATCTTTTAATGAGTATGTGTAG", "aa": "MLFITIIYLLWINCHNSTARYLILKINKYPPKKPIKNLPFIFFRLAYYTFNIRQKIQYQQKQIYKYLISFNEYV", "aa_hexdigest": "9dc52ed2281ba2740fc7b24e17702657", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 741857, "stop": 742297, "observations": { "mutation": [ 742198 ], "stop": [ 742198 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAC9T0B4", "query_cov": 1.0, "subject_cov": 0.5068493150684932, "identity": 1.0, "score": 150.0, "evalue": 2.62e-45, "gene_start": 1, "gene_end": 74, "reference_start": 73, "reference_end": 146, "reference_sequence": "MKIVLEELKDYLIGKLYFKYKFIGVFISIFLAGWLISFITNLILALNYGYNEKLLNYKKYLDFTILTCISFLMLFITIIYLLWINCHNSTARYLILKINKYPPKKPIKNLPFIFFRLAYYTFNIRQKIQYQQKQIYKYLISFNEYV" }, "paralog": false, "identity": 0.9863013698630136, "score": 292.0, "evalue": 7.81e-104, "description": "Internal stop codon at 742198. Nonsense mutation around 742198.", "psc": { "uniref90_id": "UniRef90_A0AAC9T0B4", "uniref50_id": "UniRef50_A0AAC9T0B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAC9T0B4", "UniRef:UniRef50_A0AAC9T0B4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T0B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T0B4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9245.982, "isoelectric_point": 9.956448936462401 }, "id": "GPKLONFDEH_1277", "locus": "GCA000006625_01277" }, { "type": "cds", "sequence": "contig_1", "start": 742334, "stop": 742945, "strand": "-", "frame": 3, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "COG:COG1435", "COG:F", "EC:2.7.1.21", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897", "KEGG:K00857", "SO:0001217", "UniRef:UniRef50_Q9PPP5", "UniRef:UniRef90_Q9PPP5" ], "nt": "ATGTTTGCTGGAAAAACAGCAGAGTTAATTCGTCGCTTACATCGTTTAGAATATGCAGATGTTAAATATTTGGTTTTTAAACCTAAAATTGATACTAGATCTATTAGAAATATCCAATCACGAACAGGAACGTCTTTACCATCAGTTGAAGTAGAAAGCGCACCAGAAATTTTAAATTATATTATGTCAAACAGTTTTAATGATGAAACAAAAGTTATTGGCATTGATGAGGTTCAATTTTTTGATGATCGGATTTGTGAAGTTGCAAATATTTTAGCAGAAAATGGTTTTGTTGTTATTATTTCAGGATTAGATAAAAATTTTAAAGGCGAGCCTTTTGGTCCAATTGCTAAACTTTTTACTTATGCTGATAAAATTACAAAACTAACAGCGATATGTAATGAGTGTGGCGCTGAAGCAACGCATTCATTGCGTAAGATTGATGGTAAACACGCTGACTATAATGATGATATTGTTAAAATTGGCTGTCAGGAATTCTATTCTGCTGTTTGTCGTCATCATCATAAGGTACCTAATCGTCCTTATTTAAATAGTAATTCTGAGGAATTTATTAAATTTTTTAAAAATAAAAAACGAAATAAAAATATATAA", "aa": "MFAGKTAELIRRLHRLEYADVKYLVFKPKIDTRSIRNIQSRTGTSLPSVEVESAPEILNYIMSNSFNDETKVIGIDEVQFFDDRICEVANILAENGFVVIISGLDKNFKGEPFGPIAKLFTYADKITKLTAICNECGAEATHSLRKIDGKHADYNDDIVKIGCQEFYSAVCRHHHKVPNRPYLNSNSEEFIKFFKNKKRNKNI", "aa_hexdigest": "89ef5dc5465ba9f0e2bb9d77f37d7a29", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PPP5", "query_cov": 1.0, "subject_cov": 0.8353909465020576, "identity": 1.0, "score": 412.0, "evalue": 4.96e-145, "valid": true, "gene": "tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_Q9PPP5", "cog_id": "COG1435", "cog_category": "F", "kegg_orthology_id": "K00857", "go_ids": [ "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPP5" ], "product": "Thymidine kinase" }, "genes": [ "tdk" ], "id": "GPKLONFDEH_1278", "locus": "GCA000006625_01278" }, { "type": "cds", "sequence": "contig_1", "start": 743012, "stop": 743215, "strand": "-", "frame": 3, "gene": null, "product": "Putative GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0B7IGQ7" ], "nt": "ATGAGTGCGCCTGAATGTGCAGGAATTATTCATAGCGATTTTGAAAAAGGTTTTATTAAAGCTGAAATTATGCATTATGATGATTTAATATCATTAGGAAGTGAGCAAGCTGTGAAAGAAGCGGGAAAAATAAGAATGGAGGGAAAGTCATATTTAATGAAAGATGGCGATATTTGCAATTTTAGATTCAATGTGACAAAATAA", "aa": "MSAPECAGIIHSDFEKGFIKAEIMHYDDLISLGSEQAVKEAGKIRMEGKSYLMKDGDICNFRFNVTK", "aa_hexdigest": "a8244530075f4e53191e95f363c5a3f7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0B7IGQ7", "query_cov": 0.9552238805970149, "subject_cov": 0.8311688311688312, "identity": 0.672, "score": 97.8, "evalue": 1.9e-25, "valid": false, "product": "Putative GTP-binding protein", "uniref50_id": "UniRef50_A0A0B7IGQ7" }, "pscc": { "uniref50_id": "UniRef50_A0A0B7IGQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0B7IGQ7" ], "product": "Putative GTP-binding protein" }, "genes": [], "id": "GPKLONFDEH_1279", "locus": "GCA000006625_01279" }, { "type": "cds", "sequence": "contig_1", "start": 743231, "stop": 744118, "strand": "-", "frame": 3, "gene": "ychF", "product": "Ribosome-binding ATPase YchF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P37518" ], "nt": "ATGTCGTTATCAGCAGGTCTTGTTGGTTTGCCAAACGTTGGTAAATCCACATTATTTAATACAATTACAAATTCTAGAGTTGAGGCAGCTAATTATCCTTTTGCAACTATCGAACCAAATATTGGAATTGTTAATATTAATGATCCGCGTTTAAAACGATTAGCAAGCTTAGTTGTGCCTGATAAAATTGTGCCGACCACTTTCAAATTTGTTGATATTGCAGGTCTTGTTAAAGGCGCTTCGCAAGGTGAAGGACTAGGAAACCAATTTTTAAATAATATTCGCGATGTTGATGCTATTTGTCATGTCGTTCGCTGTTTTGATGATAAATCTATTACTCATGTTCATAATAAAATTGACGCATTATCTGATATTCATGTTATTAATTATGAATTAATATTATCTGACCTTGATATTATTGAAAAACGAATTAGTCGTATTAAAAAAAAGGCTGATTCTGGCGATAAAGATGCTAGAAGAGAATATCTTATTTTATTTAACATTGAAGAAGCTTTAAAAAAGGGTCAATTAGCATCAAGTGTTAAATTAACCAAAGAAGAATTGAAAATCATTAATTCTTTCAATTTAATAACTTTAAAACCAATTTTATATATTGCAAATATTTCAGAAAATGAGATTTCAAATCCTGATTTAAATAAAGAATATTTAAAAGTAAAAAAGTATGCTCAAGATAATGATGCTAGATGCGTTGCTTTGTCAGCAAAAATTGAATATGAAATTAGCATTTTAGATGAAGAATCAAAATCAATATTTATTCAAGATTTAGGTATTAAAACAACAGGATTAGATGAAATTGTAAAGCAAACCTATGATTTATTAAATTTAAAAACTTATTTTACATATGGAAAACAAGAAGTACGTGCTTGA", "aa": "MSLSAGLVGLPNVGKSTLFNTITNSRVEAANYPFATIEPNIGIVNINDPRLKRLASLVVPDKIVPTTFKFVDIAGLVKGASQGEGLGNQFLNNIRDVDAICHVVRCFDDKSITHVHNKIDALSDIHVINYELILSDLDIIEKRISRIKKKADSGDKDARREYLILFNIEEALKKGQLASSVKLTKEELKIINSFNLITLKPILYIANISENEISNPDLNKEYLKVKKYAQDNDARCVALSAKIEYEISILDEESKSIFIQDLGIKTTGLDEIVKQTYDLLNLKTYFTYGKQEVRA", "aa_hexdigest": "4a9ae4ef85b5c7e2970944e1f0bd785e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q9PPP4", "query_cov": 1.0, "subject_cov": 0.8016304347826086, "identity": 0.8170000000000001, "score": 475.0, "evalue": 1.3e-166, "valid": false, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P37518", "cog_id": "COG0012", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P37518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P37518" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "GPKLONFDEH_1280", "locus": "GCA000006625_01280" }, { "type": "cds", "sequence": "contig_1", "start": 744129, "stop": 744401, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01116.26" ], "nt": "GTGAATAAACCAATTGTTTTACACGGAGGTTCTGGTATTAGTGATGAACAAATTTTAAAAGCAATTAATTTGGGTGTAGCAAAAATTAATATTAATACAGAATGTCAAATTGCATTTTCAAATGCATTACAAGATCACTTAACAAAAGCAGGTGATTTAATTCTTTCTAAACAGTATGATCCACGAAAAATTTTGGCTTATGGTGTGGACGCAATTAAAAATACAATCATTGATAAATTCACTAAGTTTAATAGTTTAAATAAAATAAAATAA", "aa": "MNKPIVLHGGSGISDEQILKAINLGVAKININTECQIAFSNALQDHLTKAGDLILSKQYDPRKILAYGVDAIKNTIIDKFTKFNSLNKIK", "aa_hexdigest": "561444c70e1f6bca7382827cfff41777", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002BE51033", "query_cov": 0.9444444444444444, "subject_cov": 0.8854166666666666, "identity": 0.529, "score": 90.5, "evalue": 6.35e-22, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A356BXS8", "kegg_orthology_id": "K01624" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01116.26", "name": "F_bP_aldolase", "length": 87, "aa_cov": 0.9666666666666667, "hmm_cov": 0.896551724137931, "evalue": 9.73754599061733e-23, "score": 76.60188293457031, "start": 2, "stop": 88 } ], "seq_stats": { "molecular_weight": 9895.41739999999, "isoelectric_point": 9.336649894714355 }, "id": "GPKLONFDEH_1281", "locus": "GCA000006625_01281" }, { "type": "cds", "sequence": "contig_1", "start": 744444, "stop": 744830, "strand": "-", "frame": 2, "gene": null, "product": "class II fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003593948A" ], "nt": "ATGGGTGGTTATGATAATTGTTATGCAATGGTTGTTAATTTAATAAAACAAATGAATATTAAAACTCCTGTTTGTTTGCATTTAGATCATGGTACATATGAAGGATGTATAAAAGCTATTAATGCTGGTTATTCCTCAATTATGTATGATGGATCAAAACTTTCGATCGAAGAAAACATTAAAAACACTAAAGAACTTTTGACAATAACTAAATTAAAAGGTGTATCAGTAGAAGTTGAAGTTGGTTCAATTGGTGGGACAGAAGATGGGATTACTTCAGAAGGTGAATTAGCTAATATCGACGATTGTTATCAAATGTGTTTATTAGATATTGATATGTTAGCGTGTGGTATTGGAAATATTCACGGAATATATCCTAAAAATTGA", "aa": "MGGYDNCYAMVVNLIKQMNIKTPVCLHLDHGTYEGCIKAINAGYSSIMYDGSKLSIEENIKNTKELLTITKLKGVSVEVEVGSIGGTEDGITSEGELANIDDCYQMCLLDIDMLACGIGNIHGIYPKN", "aa_hexdigest": "bef560df32278171a6b639850d137462", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI003593948A", "query_cov": 0.8203125, "subject_cov": 0.8974358974358975, "identity": 0.514, "score": 112.0, "evalue": 7.48e-30, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_UPI003593948A", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_UPI003593948A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003593948A" ], "product": "class II fructose-bisphosphate aldolase" }, "genes": [], "id": "GPKLONFDEH_1282", "locus": "GCA000006625_01282" }, { "type": "cds", "sequence": "contig_1", "start": 745006, "stop": 745230, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00291355F0", "UniRef:UniRef90_UPI00291355F0" ], "nt": "ATGTATGAAGTTGGTTTATACAAAGATAGTGACCGTGAAGAAGATGAACATGAAATTGTTAAAAATGATAAATTAACACAATCAAAAGAACAAGAGGAAAGTGATGAAGTAAGCTCTTTATCTGATTTTGTTGGCTACGAAGATGAAGATGAAGAATTTAAAAATAATAATACTTCTAATAAAGATGAAATAGAAGACGAAGAGGAATTAGAAGAGGAAGAATAA", "aa": "MYEVGLYKDSDREEDEHEIVKNDKLTQSKEQEESDEVSSLSDFVGYEDEDEEFKNNNTSNKDEIEDEEELEEEE", "aa_hexdigest": "4c52f81b209b23671e051c28412918da", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00291355F0", "query_cov": 0.9459459459459459, "subject_cov": 0.8974358974358975, "identity": 0.914, "score": 82.8, "evalue": 2.29e-19, "valid": true, "uniref50_id": "UniRef50_UPI00291355F0" }, "pscc": { "uniref50_id": "UniRef50_UPI00291355F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00291355F0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8814.911800000002, "isoelectric_point": 4.0500284194946286 }, "id": "GPKLONFDEH_1283", "locus": "GCA000006625_01283" }, { "type": "cds", "sequence": "contig_1", "start": 745282, "stop": 745488, "strand": "-", "frame": 1, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "COG:COG3343", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355", "KEGG:K03048", "SO:0001217", "UniRef:UniRef50_Q9PPP2", "UniRef:UniRef90_Q9PPP2" ], "nt": "ATGAGCCGTCAATTAGTTGATATTGCTTATAATGCAATAAAAAATAATAAAATTTATAACAAAAAACCATTTACTTTTGATGATATTATTAATGAAATTGTCAAGAATAGTAATGTAAATTTAGTAGAAGTTAATTCACAATTAGGTGTTTTATATACAACTTTAATACAAGATACACGTTTTATTTCAATTGGAGATTTAGAATGA", "aa": "MSRQLVDIAYNAIKNNKIYNKKPFTFDDIINEIVKNSNVNLVEVNSQLGVLYTTLIQDTRFISIGDLE", "aa_hexdigest": "9703b99f5e6ae61f4301c6058f325428", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 745066, "stop": 745488, "observations": { "mutation": [ 745284 ], "stop": [ 745284 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPP2", "query_cov": 1.0, "subject_cov": 0.4146341463414634, "identity": 1.0, "score": 135.0, "evalue": 4.25e-39, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSRQLVDIAYNAIKNNKIYNKKPFTFDDIINEIVKNSNVNLVEVNSQLGVLYTTLIQDTRFISIGDLEWNLRERLSLDEITKINNAMYEVGLYKDSDREEDEHEIVKNDKLTQSKEQEESDEVSSLSDFVGYEDEDEEFKNNNTSNKDEIEDEEELEEELEEEE" }, "paralog": false, "identity": 0.9857142857142858, "score": 270.0, "evalue": 7.65e-95, "description": "Internal stop codon at 745284. Nonsense mutation around 745284.", "psc": { "uniref90_id": "UniRef90_Q9PPP2", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_Q9PPP2", "cog_id": "COG3343", "cog_category": "K", "kegg_orthology_id": "K03048", "go_ids": [ "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPP2", "EC:2.7.7.6", "UniRef:UniRef50_Q9PPP2", "COG:COG3343", "COG:K", "KEGG:K03048", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPP2" ], "product": "Probable DNA-directed RNA polymerase subunit delta" } }, "id": "GPKLONFDEH_1284", "locus": "GCA000006625_01284" }, { "type": "cds", "sequence": "contig_1", "start": 745563, "stop": 745868, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGATCGTTTGCTAATGAACATGATTTTATTTTAAAAACTTTAGCAACAAATTGGTTACAAAATAAAGATTTTGTAACTTTTGAATATGAATATGGTAAAGATAAATCAAAAGATCAAAAGATTATTCATAAAAAATATTTTTATGAAACTATTACTTCAAATAAACAAATTTATGATTTTAATGAACCGGTTTTTTTATATACAGAAAACAAAAATAATGATATTAAATTATTAAAACTTGAAGATAAGAGTTTAATAATTGCTGCTTTAAAATCAATTAAACAACCACGAATAAAAAAATGA", "aa": "MRSFANEHDFILKTLATNWLQNKDFVTFEYEYGKDKSKDQKIIHKKYFYETITSNKQIYDFNEPVFLYTENKNNDIKLLKLEDKSLIIAALKSIKQPRIKK", "aa_hexdigest": "1854c131af622f0784a41367d18a3f37", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12170.863099999982, "isoelectric_point": 9.323498344421388 }, "id": "GPKLONFDEH_1285", "locus": "GCA000006625_01285" }, { "type": "cds", "sequence": "contig_1", "start": 745935, "stop": 746081, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAAATGTTTATTTAAATAAAACACCAACAACCTATGATATTTTAATGAAAAAGACTTTAGCAAGAGTGCATTTTTTATACCAAAAACAAAAAAAATATATATTCAAAAATTCACAATTATTAACAATTTTAAAACCATGA", "aa": "MFENVYLNKTPTTYDILMKKTLARVHFLYQKQKKYIFKNSQLLTILKP", "aa_hexdigest": "53dd1193d445544611a7d0c8cc5dbcfa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5851.964800000001, "isoelectric_point": 9.998740196228027 }, "id": "GPKLONFDEH_1286", "locus": "GCA000006625_01286" }, { "type": "cds", "sequence": "contig_1", "start": 746118, "stop": 746279, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAGGTTATTTCATCTCAAATTGATGCAGATCGTTTAGATTATTTGCCACGTGATTCTCATTTTACAGGAACCAAGTATGGAAATATAGACTATGATAATATCTTTAAAAAGGCAATAGTTTTTAACGATCAATTATGTTTTTCAAAACACTCTTGA", "aa": "MQQVISSQIDADRLDYLPRDSHFTGTKYGNIDYDNIFKKAIVFNDQLCFSKHS", "aa_hexdigest": "683adfa00052afb65cfa7f22b9a55e5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6184.835300000001, "isoelectric_point": 5.977495384216309 }, "id": "GPKLONFDEH_1287", "locus": "GCA000006625_01287" }, { "type": "cds", "sequence": "contig_1", "start": 746280, "stop": 746387, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTACTAATCCTAAAAGTGAAATTAATAAAATTTTAATTAAACATAATATCAATCCAGTTGATGTTGCTAATATAATTGATCATAAATCCAAAAAAATGTGA", "aa": "MIITNPKSEINKILIKHNINPVDVANIIDHKSKKM", "aa_hexdigest": "dde3bb1c436df29535245facd69c9010", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.7687000000005, "isoelectric_point": 9.699735832214355 }, "id": "GPKLONFDEH_1288", "locus": "GCA000006625_01288" }, { "type": "cds", "sequence": "contig_1", "start": 746762, "stop": 747313, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "ATGATTAAAAATTTTGATATCAATAATTTATCAAAATCCCCCGCCTTTTTTGATATCAATAAAATGAATTGGTTTTCAACACAATATTTTAATAATATTACCCAAGAAGAATTTATCAATTTTATAAAGAAACATTCATTAACTAAAGAATTAGTTTTGAATGATTATACTTTTATTAATAAATGTCTATTATTTAAATCTCATATTATTAATTTAAAACAACTAATTGATTTAGTAATTGAACAATTTAATTGTGATAAAAAAGTATTAGCATCAGATGTAGATTATATTAAAAAAAACCAATTAATAACTGTTGTTCGTGTTTTTTATGAACAATTAATAATTAATGATGAATTTAATGAAGAATTTATAAAAGAAATAATTAAAAAAGTTCAAATAATAACTAATAACAAAGGTGCTAATTTATACATGCCAATTCGTATTGCAACTACATTTAGTTCGCATGGTCCAGAATTAGCTAAAACAATTTGTTATTTAGGACGCGAAAAAGTATTAAAAAATTTAATAAATATTTTAAAAATTTTGGATTAA", "aa": "MIKNFDINNLSKSPAFFDINKMNWFSTQYFNNITQEEFINFIKKHSLTKELVLNDYTFINKCLLFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQLITVVRVFYEQLIINDEFNEEFIKEIIKKVQIITNNKGANLYMPIRIATTFSSHGPELAKTICYLGREKVLKNLINILKILD", "aa_hexdigest": "85bb9578639dac22ae07c288b0660363", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 118, "aa_cov": 0.6229508196721312, "hmm_cov": 1.0, "evalue": 1.4137542921295673e-12, "score": 43.794464111328125, "start": 62, "stop": 175 } ], "seq_stats": { "molecular_weight": 21562.018100000023, "isoelectric_point": 8.847850608825684 }, "id": "GPKLONFDEH_1289", "locus": "GCA000006625_01289" }, { "type": "cds", "sequence": "contig_1", "start": 747353, "stop": 748021, "strand": "-", "frame": 3, "gene": "gltX", "product": "glutamate--tRNA ligase", "db_xrefs": [ "COG:COG0008", "COG:J", "EC:6.1.1.17", "GO:0000049", "GO:0000166", "GO:0004818", "GO:0005524", "GO:0005829", "GO:0006424", "GO:0008270", "SO:0001217", "UniRef:UniRef50_P47700", "UniRef:UniRef90_Q9PPP0" ], "nt": "ATGGGTATTATTCCAGACGAATCAATTCGTAATCCTAAAGCTTTTGGCCCATATATCCAATCTGAAAAATTAAAACATTATGAAAAATTAGCATTAGATCTAGTTGATCAAAAAAAAGCTTATTTTTGTTTTTGTAGTAAAGAACAATTAGATGCAGATCGCGAATTAGCTGAAAAATCACATCAAACACCTAAATACAAACGGCACTGTTTAAATTTAGATAAAAAAACAATTGAAAGTAACTTATTACAAAATAAAGAATACACAATTCGTTTAAAAATTAATGAAAATATGGAATATAGCTGGGATGATTTAATTCGGGGTAAAATTAGTATTCCAGGTAGTGCTTTAACTGATCCTGTCATTTTAAAATCAAATAAGATTGCAATGTATAATTTTGCAGTTGTAATTGACGATTATGAAATGCAAATTTCACATGTCATTCGTGGAGAAGAGCATATTTCTAATACACCTTATCAATTAGCGATTGCTCAGGCCTTAAATTATGATATAACAAAAATTAAATATGGTCATTTGTCAATTATTGTTGATGAGACAGGAAAAAAATTATCAAAGCGAAATTTATCTTTAAAACAGTTTGTGTCTGATTATGAAAAAGATGGTTATTGGCCACATGCTATTACAAACTTTGTTGCATTATTAGGTTGA", "aa": "MGIIPDESIRNPKAFGPYIQSEKLKHYEKLALDLVDQKKAYFCFCSKEQLDADRELAEKSHQTPKYKRHCLNLDKKTIESNLLQNKEYTIRLKINENMEYSWDDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDITKIKYGHLSIIVDETGKKLSKRNLSLKQFVSDYEKDGYWPHAITNFVALLG", "aa_hexdigest": "f886159273085b45536e7b62cf73271b", "start_type": "ATG", "rbs_motif": null, "genes": [ "gltX" ], "truncated": "both", "pseudogene": { "start": 747050, "stop": 748210, "observations": { "mutation": [ 747355, 748024 ], "stop": [ 747355, 748024 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPP0", "query_cov": 1.0, "subject_cov": 0.4605809128630705, "identity": 1.0, "score": 446.0, "evalue": 8.84e-155, "gene_start": 1, "gene_end": 222, "reference_start": 64, "reference_end": 285, "reference_sequence": "MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYGGDFIIRIEDTDIERNVEGGINSQLDFLAWMGIIPDESIRNPKAFGPYIQSEKLKHYEKLALDLVDQKKAYFCFCSKEQLDADRELAEKSHQTPKYKRHCLNLDKKTIESNLLQNKEYTIRLKINENMEYSWDDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDITKIKYGHLSIIVDETGKKLSKRNLSLKQFVSDYEKDGYWPHAITNFVALLGWSPKNNDEIMSLETMIKNFDINNLSKSPAFFDINKMNWFSTQYFNNITQEEFINFIKKHSLTKELVLNDYTFINKCLLFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQLITVVRVFYEQLIINDEFNEEFIKEIIKKVQIITNNKGANLYMPIRIATTFSSHGPELAKTICYLGREKVLKNLINILKILD" }, "paralog": false, "identity": 0.9948186528497409, "score": 773.0, "evalue": 3.45e-286, "description": "Internal stop codon at 747355, 748024. Nonsense mutation around 747355, 748024.", "psc": { "uniref90_id": "UniRef90_Q9PPP0", "gene": "gltX", "product": "glutamate--tRNA ligase", "ec_ids": [ "6.1.1.17" ], "uniref50_id": "UniRef50_P47700", "cog_id": "COG0008", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0000166", "GO:0004818", "GO:0005524", "GO:0005829", "GO:0006424", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPP0", "EC:6.1.1.17", "UniRef:UniRef50_P47700", "COG:COG0008", "COG:J", "GO:0000049", "GO:0000166", "GO:0004818", "GO:0005524", "GO:0005829", "GO:0006424", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47700", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47700" ], "product": "Glutamate--tRNA ligase" } }, "id": "GPKLONFDEH_1290", "locus": "GCA000006625_01290" }, { "type": "cds", "sequence": "contig_1", "start": 748022, "stop": 748210, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00749.27" ], "nt": "ATGAAAATTAGAACACGTTATGCTCCATCACCAACCGGTTATTTACATATTGGAGGAGCGCGAACAGCTTTATTTAATTATTTACTAGCAAAAGCTTATGGTGGGGATTTTATTATTCGTATTGAAGATACTGATATTGAAAGAAATGTGGAAGGGGGAATTAATTCCCAACTAGATTTTTTAGCATGA", "aa": "MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYGGDFIIRIEDTDIERNVEGGINSQLDFLA", "aa_hexdigest": "44203cadf53c21804e2d95111d2f1714", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0016AA1FE4", "query_cov": 0.967741935483871, "subject_cov": 0.8333333333333334, "identity": 0.6829999999999999, "score": 85.1, "evalue": 1.46e-20, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A5J4P5N7" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00749.27", "name": "tRNA-synt_1c", "length": 60, "aa_cov": 0.967741935483871, "hmm_cov": 1.0, "evalue": 1.7052674701815466e-29, "score": 98.28517150878906, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 6904.773399999999, "isoelectric_point": 6.534062767028809 }, "id": "GPKLONFDEH_1291", "locus": "GCA000006625_01291" }, { "type": "cds", "sequence": "contig_1", "start": 748431, "stop": 748997, "strand": "-", "frame": 2, "gene": "ispE", "product": "4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase", "db_xrefs": [ "COG:COG1947", "COG:I", "EC:2.7.1.148", "GO:0005524", "GO:0016114", "GO:0019288", "GO:0050515", "KEGG:K00919", "SO:0001217", "UniRef:UniRef50_Q9PPN9", "UniRef:UniRef90_Q9PPN9" ], "nt": "ATGAAAGTAAAGTCATTCGCAAAAATTGATTTAGGATATAGTGTTTATAAAAAACAAAAGAATTTTACAAAACACGATTTTGAATCAATTTTTATACTTGTTGAAAATATTTATGACGATATTGAAATTACTAAAATAGAAAAAAATATTGATGATGTTCATTACTACAATGAAACCAATGAAATTTATGTTTATAGCCGTTTAGTTCATAAGACATTAGAATGGATTCGTCATACTTATCATATTAAAAATCATTATCGAATTAATATTAAAAAAAGAATTCCAATTGGTGCAGGCTTAGGTGGTGGTTCATCAAACGCTGCAGCCATTATGAAATATATTCTAGAATTTGAGGGTATTAAAGAAATTAATTATAAAGATGTTGTTAATAAATTAGGTGCTGATATTCCCTTTTTTTTATCAGGCTATAAAACAGCTTATATAAGTGATTACGGAAGTGTTTTAGAGGATTTGACTGGTCAATTTAAATTAAATTATGAAGTTTATTTAATGAATGTTAATGTAAATACAAAAATTGTTTTTGAAAAATTTGATGATAATTCATGA", "aa": "MKVKSFAKIDLGYSVYKKQKNFTKHDFESIFILVENIYDDIEITKIEKNIDDVHYYNETNEIYVYSRLVHKTLEWIRHTYHIKNHYRINIKKRIPIGAGLGGGSSNAAAIMKYILEFEGIKEINYKDVVNKLGADIPFFLSGYKTAYISDYGSVLEDLTGQFKLNYEVYLMNVNVNTKIVFEKFDDNS", "aa_hexdigest": "fb3a3ad02f5ee6e62aead7acc36ccaa9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ispE" ], "truncated": "5-prime", "pseudogene": { "start": 748200, "stop": 748997, "observations": { "mutation": [ 748433 ], "stop": [ 748433 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9PPN9", "query_cov": 1.0, "subject_cov": 0.7094339622641509, "identity": 1.0, "score": 377.0, "evalue": 4.32e-131, "gene_start": 1, "gene_end": 188, "reference_start": 1, "reference_end": 188, "reference_sequence": "MKVKSFAKIDLGYSVYKKQKNFTKHDFESIFILVENIYDDIEITKIEKNIDDVHYYNETNEIYVYSRLVHKTLEWIRHTYHIKNHYRINIKKRIPIGAGLGGGSSNAAAIMKYILEFEGIKEINYKDVVNKLGADIPFFLSGYKTAYISDYGSVLEDLTGQFKLNYEVYLMNVNVNTKIVFEKFDDNSWHVIKNNFKTIIKNLKENIVVNIHNDLQEYCFELYPNIKYKYNELLSDGFYTILSGAGSSFIRIKLKNKEDLIINEN" }, "paralog": false, "identity": 0.9962264150943396, "score": 534.0, "evalue": 1.08e-195, "description": "Internal stop codon at 748433. Nonsense mutation around 748433.", "psc": { "uniref90_id": "UniRef90_Q9PPN9", "gene": "ispE", "product": "4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase", "ec_ids": [ "2.7.1.148" ], "uniref50_id": "UniRef50_Q9PPN9", "cog_id": "COG1947", "cog_category": "I", "kegg_orthology_id": "K00919", "go_ids": [ "GO:0050515", "GO:0005524", "GO:0019288", "GO:0016114" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9PPN9", "EC:2.7.1.148", "UniRef:UniRef50_Q9PPN9", "COG:COG1947", "COG:I", "KEGG:K00919", "GO:0050515", "GO:0005524", "GO:0019288", "GO:0016114" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPN9" ], "product": "4-diphosphocytidyl-2-C-methyl-D-erythritol kinase" } }, "id": "GPKLONFDEH_1292", "locus": "GCA000006625_01292" }, { "type": "cds", "sequence": "contig_1", "start": 749031, "stop": 749552, "strand": "-", "frame": 2, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "EC:2.1.1.182", "GO:0000179", "GO:0006364", "SO:0001217", "UniRef:UniRef50_Q9PPN8", "UniRef:UniRef90_UPI001152E4FE" ], "nt": "TTGATTTTAGATTACAATAATACTCAAAAAATTCAAAAAATTAAAGTTGTTGCAAATCTTCCATATGCAATATCTTCAAAGATAGTTTTAAAAATTATTCAATCAAAATTAATTAATGATGCTTATATTATGGTTCAAAAAGAAATGGCTGAACGAATTGGTGCTAAAGTAAATACACGTGGTTATAATGCTTTTACTGTTTTAGTACAATTATTCTGTAAAACAAAAATTCTATTCGAAGTTAATGCTAAAGAATTTCATCCCCAACCTAAAGTTCAAAGTGCTGTCATTCATCTTGAAAATCTTCATAATAGTGTGAATTTTAATATTGAAGAATTAGGTAAATTTTTACGGATTTGTTTTTTAAATAAACGAAAAAAGTTAAAAAATAACTTATCTAATATTTATGATATTAAAATAATTAATCAAATGTTTATTGATTATAATTTAGATATGAATTTACGAGCTGAAAACATTGAACCAAAAATGTTTCTAAAACTCTTTAATTATTTGAATAGATAA", "aa": "MILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMNLRAENIEPKMFLKLFNYLNR", "aa_hexdigest": "14c8706d1ddfbaa9d5188368a92fd627", "start_type": "TTG", "rbs_motif": null, "genes": [ "rsmA" ], "truncated": "3-prime", "pseudogene": { "start": 749031, "stop": 749852, "observations": { "mutation": [ 749705 ], "stop": [ 749705 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001152E4FE", "query_cov": 1.0, "subject_cov": 0.6245487364620939, "identity": 0.9940000000000001, "score": 337.0, "evalue": 1.21e-115, "gene_start": 1, "gene_end": 173, "reference_start": 105, "reference_end": 277, "reference_sequence": "MNKSFIKNKLKQESFVPSKKMGQNFLLSNNIKNKIVDVANINKDDLILEIGPGWGAITEILVQKTNILIAIELDKRLYAHLKTYIKTSNFNIINNDVLCVDLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYDIKIINQMFIDYNLDMNLRAENIEPKMFLKLFNYLNR" }, "paralog": false, "identity": 0.9926739926739927, "score": 530.0, "evalue": 3.13e-194, "description": "Internal stop codon at 749705. Nonsense mutation around 749705.", "psc": { "uniref90_id": "UniRef90_UPI001152E4FE", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_Q9PPN8", "go_ids": [ "GO:0000179", "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001152E4FE", "EC:2.1.1.182", "UniRef:UniRef50_Q9PPN8", "GO:0000179", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPN8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPN8" ], "product": "Ribosomal RNA small subunit methyltransferase A" } }, "id": "GPKLONFDEH_1293", "locus": "GCA000006625_01293" }, { "type": "cds", "sequence": "contig_1", "start": 749703, "stop": 749864, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00398.26" ], "nt": "TTGAATAAATCATTCATTAAAAATAAACTCAAGCAAGAAAGTTTTGTTCCATCAAAAAAAATGGGGCAAAACTTCTTATTGTCTAATAACATAAAAAATAAAATTGTAGATGTGGCGAATATTAATAAAGATGATCTTATATTAGAAATTGGTCCAGGGTGA", "aa": "MNKSFIKNKLKQESFVPSKKMGQNFLLSNNIKNKIVDVANINKDDLILEIGPG", "aa_hexdigest": "06a7f9ce0264fe3f07b891cada4ddd8c", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00398.26", "name": "RrnaAD", "length": 35, "aa_cov": 0.660377358490566, "hmm_cov": 1.0, "evalue": 1.0930756333175905e-09, "score": 33.356536865234375, "start": 19, "stop": 53 } ], "seq_stats": { "molecular_weight": 5974.9468000000015, "isoelectric_point": 9.69941349029541 }, "id": "GPKLONFDEH_1294", "locus": "GCA000006625_01294" }, { "type": "cds", "sequence": "contig_1", "start": 749945, "stop": 750196, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATACGATTGCAGAATAGTAAAGATCGCATTGAAGAATCAGTATAAACCAATCCCGGAAGCAGAAATTGCTACTACAACAGCTAAAACAATTGCAATAATGTTTTGTGTCATTCGTACACGTTTTAACTGTTGTTTATTTTTAGCACCAACAGTTGTGGCATTACGATTACGTTTTCTAGCTAATAGTTGTGGTATTTTTTGGGATAAGATTTGTACTGGAATAATAATTAATAAGAAGAAAATATAA", "aa": "MIYDCRIVKIALKNQYKPIPEAEIATTTAKTIAIMFCVIRTRFNCCLFLAPTVVALRLRFLANSCGIFWDKICTGIIINKKKI", "aa_hexdigest": "6c85bcd9a53529f4414fb4ca1d10e84a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9455.49129999999, "isoelectric_point": 9.64854793548584 }, "id": "GPKLONFDEH_1295", "locus": "GCA000006625_01295" }, { "type": "cds", "sequence": "contig_1", "start": 750256, "stop": 750528, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGATCTCAGCACGTGCAACTATCCAAACAGAAAGAATTTCTGAAATTCAAGGAAAAATTGCAGAAATTAATGCAAAATATAAAGACGCAAAAGATATGCAGTCTCGTCAAAAAAAACAAATGGAAACAAAAGAGTTATATCAAAAACATAACGTTAAACCTTTAGCTCCTTTTGAAAGTATGATTATTACATTGCCGATCTTTTTAATTATTTATCGTGTTGTTACAATTTTAAGACCACTTAAGTTTATTAGTATTTTTTATATTTGA", "aa": "MLISARATIQTERISEIQGKIAEINAKYKDAKDMQSRQKKQMETKELYQKHNVKPLAPFESMIITLPIFLIIYRVVTILRPLKFISIFYI", "aa_hexdigest": "56212c81309cbf1a998a80ca3fd18d64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10585.562999999991, "isoelectric_point": 9.87057704925537 }, "id": "GPKLONFDEH_1296", "locus": "GCA000006625_01296" }, { "type": "cds", "sequence": "contig_1", "start": 750592, "stop": 750690, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGATTTTTCATTTGATTATGGTCCTTTCTATGCTTTATTTGTTTGGCCAATTGCACAAATTTTATTACATTTTATGTATGCAACGAGAGATTGA", "aa": "MSDFSFDYGPFYALFVWPIAQILLHFMYATRD", "aa_hexdigest": "464809b5a274e051a18d1194fe650034", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3863.4151, "isoelectric_point": 4.647690391540528 }, "id": "GPKLONFDEH_1297", "locus": "GCA000006625_01297" }, { "type": "cds", "sequence": "contig_1", "start": 750811, "stop": 751017, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATCTGTTGATAAACAAAATTTAACAAATAGAATGCGGATTTCTGTTTCACATTTTGCTGGAGCATCGAATGCAAATTCGATAAAAAAAGAACGTAAGAAAAAAATTTTTAATGTTTTATTAAAAGTTTTTAAAATCATCGTTTATGCATTTTTCTTAGGTATTGGCTTATATGGTTGTTTCCAAAATATGGCAAACCACTGA", "aa": "MSSVDKQNLTNRMRISVSHFAGASNANSIKKERKKKIFNVLLKVFKIIVYAFFLGIGLYGCFQNMANH", "aa_hexdigest": "100c5208ba58503446b82b1a41071a70", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7739.078499999997, "isoelectric_point": 10.362728691101072 }, "id": "GPKLONFDEH_1298", "locus": "GCA000006625_01298" }, { "type": "cds", "sequence": "contig_1", "start": 751056, "stop": 751397, "strand": "-", "frame": 2, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "COG:COG0594", "COG:J", "EC:3.1.26.5", "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781", "RefSeq:WP_006688777.1", "SO:0001217", "UniParc:UPI0000134448", "UniRef:UniRef100_Q9PPN6", "UniRef:UniRef50_Q9PPN6", "UniRef:UniRef90_Q9PPN6" ], "nt": "ATGGCTAATTTTATTAGTCTAAAAAAAAATGAAGATATACTTGATACAATTAAGAAACAACAAAAAATTCATTCTAATCAAATCGTAGTTTATTTTCGTAAAACCAATCTAAAAAATGTTCGCTTAGCTATAAGCATTTCTAAAAAAAAGTTTAAATTAGCAACACAACGCAATCGAATTCGACGCCTGATTAAAGCATGGTTTATTGCTGCTGATATTCCCATTAAAAGTTATGATATTGTAGTCTTAGTGAAACCATCGTTTATTGATGGTTCTTTTGTTTTAAATTGTAATAATCTTAAAATAATTCTTCAACGAATTATTAACAAAGAAAAAAGATAA", "aa": "MANFISLKKNEDILDTIKKQQKIHSNQIVVYFRKTNLKNVRLAISISKKKFKLATQRNRIRRLIKAWFIAADIPIKSYDIVVLVKPSFIDGSFVLNCNNLKIILQRIINKEKR", "aa_hexdigest": "cd69982e3371440b5688026fbe3951f8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134448", "ncbi_nrp_id": "WP_006688777.1", "uniref100_id": "UniRef100_Q9PPN6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134448", "RefSeq:WP_006688777.1", "UniRef:UniRef100_Q9PPN6" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPN6", "uniref90_id": "UniRef90_Q9PPN6" }, "psc": { "uniref90_id": "UniRef90_Q9PPN6", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_Q9PPN6", "cog_id": "COG0594", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPN6" ], "product": "Ribonuclease P protein component" }, "genes": [ "rnpA" ], "id": "GPKLONFDEH_1299", "locus": "GCA000006625_01299" }, { "type": "cds", "sequence": "contig_1", "start": 751403, "stop": 751549, "strand": "-", "frame": 3, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "RefSeq:WP_004026378.1", "SO:0001217", "UniParc:UPI0000133FDD", "UniRef:UniRef100_Q9PPN5", "UniRef:UniRef50_Q8EU89", "UniRef:UniRef90_Q9PPN5" ], "nt": "ATGAAAAGAACGTTTCAACCTAATAATCGTAAAAGAGCAAAAGTGCATGGTTTTCGTGCACGCATGAAAACAAAAAATGGGCGTAATGTTTTAGCTAGAAGAAGACTTAAAGGGCGTCATAGTCTAACAGTTTCTAGTGAAAAATAA", "aa": "MKRTFQPNNRKRAKVHGFRARMKTKNGRNVLARRRLKGRHSLTVSSEK", "aa_hexdigest": "2dfe555e2e7567f932d329b6ccd7168e", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0000133FDD", "ncbi_nrp_id": "WP_004026378.1", "uniref100_id": "UniRef100_Q9PPN5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FDD", "RefSeq:WP_004026378.1", "UniRef:UniRef100_Q9PPN5" ] }, "ips": { "uniref100_id": "UniRef100_Q9PPN5", "uniref90_id": "UniRef90_Q9PPN5" }, "psc": { "uniref90_id": "UniRef90_Q9PPN5", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_Q8EU89", "cog_id": "COG0230", "cog_category": "J", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EU89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EU89" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "GPKLONFDEH_1300", "locus": "GCA000006625_01300" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Ureaplasma parvum] [strain=ATCC 700970] [gcode=11] [completeness=complete] [topology=circular]", "nt": "ATGGCTAATAATTATCAAACTTTATATGATTCAGCAATAAAAAGGATTCCATACGATCTTATTTCTGATCAAGCTTATGCAATTCTACAAAATGCTAAAACTCATAAAGTTTGCGATGGTGTTTTATATATAATTGTAGCCAATGCCTTTGAAAAAAGTATTATTAACGGTAATTTTATTAACATTATTTCTAAATATCTAAGCGAAGAATTCAAAAAGGAAAATATTGTTAATTTTGAATTTATTATAGACAATGAAAAATTATTAATTAATAGCAATTTTTTAATTAAAGAAACTAATATTAAAAATCGTTTTAATTTTAGTGATGAACTTTTACGTTACAATTTTAACAATTTAGTAATTAGTAATTTTAATCAAAAAGCGATTAAGGCGATTGAAAATTTATTTTCAAATAACTATGATAATAGTTCAATGTGTAACCCTTTATTTTTATTTGGTAAAGTTGGTGTTGGTAAAACGCATATCGTGGCTGCTGCTGGTAATCGTTTTGCTAATAGTAATCCTAATTTAAAAATTTATTATTATGAAGGGCAAGATTTTTTTCGAAAGTTTTGTTCTGCTTCGTTAAAAGGGACTAGTTATGTTGAAGAGTTTAAAAAAGAAATTGCTTCAGCAGATTTATTAATTTTTGAAGATATTCAAAATATCCAATCACGTGATTCAACGGCTGAATTGTTTTTTAATATCTTTAATGATATAAAATTAAATGGTGGAAAAATTATCTTAACATCTGACCGTACACCAAACGAACTTAATGGTTTTCATAATCGAATTATTTCGAGATTAGCGTCAGGTTTGCAGTGTAAAATTTCTCAACCCGACAAAAATGAAGCTATTAAAATTATTAATAATTGGTTTGAATTCAAAAAAAAATATCAAATTACTGACGAAGCTAAAGAATATATTGCTGAAGGTTTTCACACTGATATTAGACAGATGATTGGTAATCTAAAACAAATTTGTTTTTGAGCGGACAATGATACTAATAAAGATTTAATAATCACAAAAGATTATGTAATTGAGTGTTCAGTTGAAAACGAAATTCCACTAAATATTGTTGTTAAAAAACAATTTAAACCTGAACAAATAATTGAAATTGTTGCAAAAGAATTAAATATAAAAACAGATTTAATTAAATCTTCTATTAGAAAAAATAATATAGTTTGAGCGCGTGATATTGTTTGTTATATTCTAAAAAATAAACTAAATTTAACTTTAACAGATATTGGTAAATTACTAAACGGACGAGAACACACTACAATTAGTCATAGTATTAGTAAAGTTCAAAAAATTCTTGATGATGAAAATAGTCAAGAAGCACTACAAATTAATCTAATTATCAATAAATTTTAATTTTATTAAAGAAAAGTAATCAAAACTTATTAAAATATTATATATTTATATATAGTGTATTTTCGTGTTTTTGAACTCAAAACATAAAATTACTAATTTTTTATTATTTAACTTATATATTGTAATAAAGGAAGAGAATTATGAGAGATATTCATCCAGTAAGCAAACCATGCGTTTATAATTGTGTAACATGTAAAAAAGAATTTATAATTGATTCTGCAGCAAAAAATACAGAAGTTGCAATTGAAGTTTGTTCAAATTGCCATACATTTTTTATTGGTAAACAAAACGCAACTACAACATTACGTGGTCGTGCTGAAAAACTAAATAATCGTTTTGAAGCTGGTTTAAATAACATTAATAAAAAACCAGAAAAGAAAAAAATACAAGGTAAATCTGAACCAAGAAAAAGTTTAAACGAATTATAATTAATCATCTTGTCATTAATTGCCAAGATTGTTTAATTCTTTTTGTATAATTAAAAATTAGTAGATTAAATAACAGGCAATTAGATTACCTGTTATTTTATTTTTACTAAAATATTTAAAACTGAGGGACTTATGGAATACAACAAAAAACTTTATGAAGCAATTGAACGAGTCGCTATTAAAAATGACGCTTTAAAAAAAGAATTAGAAACGGTTGTAACAGACTTTAAAAAAATTAAAGAGATTAATATACAGTTAAAAAAAACTACTAAAATTGCTGAAGCCTTTGCAAAATATAAACAAAAATTGGATACTGGTATAGCTGCTGAAAAAATATTAAATACAGAAAAAGATTTAGAATTAATCGAATTAGCACAGATGGATTTAGATGAAGCTAAAATTAATATTCCAATAATTGAAAATGATTTAAAAATTATGCTGTTGCCAACTGATCCTAATGATGATAAAAATGTTATTGTTGAAATGCGCCCTGCTGCTGGAGGGGATGAATCATCAATTTTTGTCGGCAATTTGTTTGATACATATCGTGCTTATGCTGAAAATAATAATTGAAAAATGAAAATTATAGAAATGACTCCGAATGCTGTTGGTTTTAGTTTTATTTCTTTTATGATTTCTGGAGAAGAAGTTTATTCACGAATGAAATTTGAGTCAGGAGTTCATCGAGTACAACGTGTACCTGCTACTGAATCTAAAGGAAGAGTACATACGTCAACAATTACAGTTGCTGTTCTTCCAGAACAAGATGAAGTTGATGTTGTAATTAATCCAACAGAATTAAGAATTGATACTTACCGTGCAAGTGGGGCTGGAGGACAGCATGTTAATAGAACAGAATCAGCTGTAAGAATTACGCATATTCCAACAGGGGTTGTTGCTGCATGTCAAGAGGGAAAATCACAAATTGAAAACCGAGAAACAGCTATGAAAATGTTAAGAGCTAAATTATGAGAAGCAGCTCAAGAACAGCAAAATGCAGAATTTGCTAATTTAAGAAAAAATCAAGTTGGTACAGGGGATCGTTCTGAAAAAATCCGTACTTACAATTATCCACAGAACCGCGTTACTGACCATCGTATTAACCTAACATTAAATAAATTAGACCAAATCATGATGGGTGAGTTAGATGAGATTATTGATGCATTAATTGCTGACGAACAAACTGGTTTAATGGCTAATTTAGATATTTAATGACCTACCATCAATTAATTTTTCAAGCATACTCACTACTTGAAAAAAAATTACGTAATCCACAAGTAGCATTTCAGTTGCTGTACGGATTAGATAATAAAATTAACGATTCTTATAGTTTTTCAAATAACCGCTTAACAATTGTAAATTCTAATTTAGAATATACATATTTTAAACTATTAGATGAATTTATTAATGGAAAACCATTAGTGAGAATTTTAGGATATGGCTATTTTTGTGCAAAGCGCTTTTATGTTGATAAAAATGTTTTTGCTTTTCGTGTTGAAACTGAATTATTAATAGATGTTGTTAATAAAGTAATTCAACAATCCACTTATAAAATTAAAAATGTTATTGATGTTTGTTGTGGTAGTGGTGTTTTAGGGTTAAGTACAAAAATGAATTTTAATCAATTGAATGTTTCATTATTAGATATTTCTATTGATGCAATTAATAATTGCAAAAAAAATGCTAAATATCATAACTTAACAGATGTAAATTTTATTCATAAAAGTATGCAAGAATACTTTTTAAACACAAAAAAACGGTTTGATTTAATTATCTGTAATCCCCCATATATCAAAAGTAATTATGAATTAAATCGTGAAGTCTTAGATTATGACCCCATTAATGCACTTATTGATTTCGATCACAAAGATGGAATTAGTTTTTATTTATTTATAATAAATAACATTAAATCAATTGCTAATGAGAAATTCACTATTATTTTTGAAATTGGTTTTGATCAAAAAGAAATTTTAGAAAAAGTTATTAAAAAAAATGAATTTCCTCTTTTTTACTACTTTATAAATGATTATAATAATCTATGTAGAATTTTAATAATAAGTAATATTAAATATGAAAATTTATAGAATAACTAATTTAAATGTGATTTATGATGCTTTAATGGCCAATAAATGTGTTCTAATCCCTACTGATACTATCATTGGTTTATTAGCAAAAAATCAAGATGTAATTTATGAAATTAAGCAACGTGATCGAAATAAGAAAATCATCCGCTTTGTAGCCGATTACAAATTACTTGGTGATTTAACTGTAGAACAAAAGCAATTTTTAGATCTTTTTTGACCAGGTTCTGTCACTATCATTAAAAATGGTATTTCTTATCGTATGCCAAATAGCCCTTATATACTAAAATTAATTCAAAAGTTAGGTCCGTTATATTGTAGTAGTGCTAATATTTCAGGTGAAGAACCAATTAAAAATCATAATGAAGCAATTTTTAAATTTGGAGCAAATAGTAAACTAATTTATGTTGAAGCACAACAACAAATTGGTGTTCCATCAACTATTGTTGACATCGATAAATGAGAATACATTCGTCGTGGTGCTAATGTTGAGATGGTAGATATGTTTATTAAAGAACTAAAAGATAATAAAACAGGGAAATAATCAATGATAAAAAAAATTTATTTTGGTAATGATCATGCTGCTTATGAAATTAAAGATCAAATTATCACTCATTTAAAACAAAAAGGTTATCAAATTATTGATGAAGGTGCACAAGCCGCATTAGGTTCTGTTGATTATAGTACATATGCATTAAAAGTTGCTAATGACGTTGTTAATGATACTAAAAATGATAGCATAGGTATTTTATTATGTGGCACGGGAATAGGTATGAATATAGCAGCAAGCAAAGTGCATGGAACGCGGGTTGCGTTAGTGTATAACGAAAGCAGTGCAAAACTAGCCAAGGAGCATAATAATGCTAACATAATTACTATAGGAGCACGTGAAAATAGTTTAAACCAAATTCTTAAAATGATTGATGATTTTTTAACAAGTAAATTTTTAGGCGAGCGTCACCAAAAACGTTTAGATATAATTGCAAAATATGAAAAAGAGCAAAATAGTTAATTTTAAAAGTTTTTTTAATTTTTTTAACATTTTGTGTTCATTTTGTAGTAATATAAAAGTGCAATAAAATATTAATATTAAAGAAAGATTATATTATGTTAAATATTTTAAATAATGTATCTAATTCTTCACTATATTCAGCCGCTTCAAATGCTACTGCGCAAACTGGTTCAAATTTGATCAATGATCTAGTTCCAGAAACATTAACCGCTTCAGGAATTAGTATCGCTATTTCTGTTTTTAGTGTTATAGGTACGATCGTTATTGCTTTGAGTGTATTGCCACAAACAATTAAAACTTTACGTGAAAAAGATACAGCTTCATTAAGTTTATTACTATTCTTGCTTAATGGTATTGCTACAGCCTTTTTAACTCTATACGGTATTGGACTTGTTACAGTTCATCCAAATTCATTTTCATTCTTAGTGGATATTAAAAACGGAATGTTCATTTATAATAGAGAAGAGTGAGTTGCTGGATACTTAATCTGCGGTATCTTCTTAATTATGGGTGAAGCATTATGTTCAGTAACTTCATTTATTGTTCTATTCTGCAAAGTTAATAACATGATTAAAGCTAAAAAAATGGGAATGAGCGAAGAAGAATATTATGAAAAACAAATTAAACCATTCCTAAAAGTGAAAGGAGCTAACTAATTATGAATTTCACTTCATTATTGCAAGATGGTATATATGAAGTTGGTAATGGTGCAATCGTTACTGACCAATCACCATATTTAGGAATTACACCTGATTATCAGGGTGCATATGGTTTCCCAACACACCCTTGAGGTATTTTCTTTCAAGTTGTTGGAGCTATTTTAGTTTTTGGTGCTTATTTACCTGCTGTTATTAAAGTACTAATTTCAAAAAGAACTGAAAATTTGGCTATTGGTATGTGAATTATTTCAATTGCTGGTTTAGGATTATTAGCGATATTTGCTTGATTAGGTGTTTCTGTAAACCCTGGAGGATTTATTCTTGTAGCTCTAAGTGAAACACTATCTTGCATTGCAAGTATAATTGTGTTTGCTTTAAAAATTGCAAATAAAGCTAAAGCTAAAGCCGCTGGTATGACTGAATTAGAATACTGCAACTTACACTATCCAATTGTTAAAAAATTACCTAAAAGATAAATTTAATACTTTTTTTAAAAAAAGAAGAAGAAAGAGAGAAGAGTTAAAACTCTTTTTTCTTTTTTTATTGTTTATTATCTATAAAACTAAGTTAATGTCTATTATAATATAAATTATAAATTTTATTTGTGGAGGTGGTTTATTGTGGAAAATAAACAAAGTACATTAAATTTAAATAACAACACAAATATTAATCAAAATTTTCTAACACAAAATATTTTAAAAACTTACGAACATAAAGATATTACAGGACTTAAAAAATTACTAAAATCAAAACGGATTGATAAAATTGTTGAAAGTCTTGAAACTATTAATGATACACAAATTATTTTATTTGTATTAGTTGCCACAAAAGATGGAATTTGTGGTGATATTTTTAAATATTTAAATACTGAATTAAAATCAAAAATCATAAATGACGCAAGTTTACAGCAATTAAGAATCATTTTATTTGAACTTTATAATGATGATGTGATTATTTTAAAAAATGATTTTCCTATGTATACTAAAAAAATCTTACTTTCATTAGACAGCCAACAAAGAGCAGCTATTAAGCAATTATCTGAATTTGATGAAGATGAAGCTGGTTCAATCATCAATTCTGACTTTTTTACAATCAATCAAAATCTTTCTATCAAAGAAGCACTAATTGAAATAAAAAGAATGTATAATGACTTTGAACAAAGTAATATTATATATGTAGTAGATAATTATAATCGTTTAAAAGGATATGTAACAATTCATTCATTACTTTTTGCAGATTCTTTTGAATTGAAAATTTCTAATGTTATTCAAGAAGATGTTTTCTATGTTCGTAGTGATGAAGATATTGATGCTGTTTTAGATATTTTCAGAAAGTATCAAATTGAACAATTAGCAGTTGTTGATAAAGATAACCAATTAATTGGTTATATTAGTGATAATGATATTTTACCCGTAATTAATGCTGAAACAACTACTGATATTTATAAAATGTATGGGATTAGCGAATTAGATTTTCCTTACATTAAATCAAGTGTTTTTGTTTTATTTAAATCACGTCTATTATGATTGGCTGTATTAATGATTTCAGCTACATGTACTGGTTTTTTAATTGATAAATTTCAAGATGTAGGACAATTAGTCACAGCAGGTTTATCAACATTAGTTATCGTACCAATCATTCCAGCAATGACGGGAACAAGTGGAAATGCTGGATCACAAGCTGCCGCCTCTGTGATTCGTGCTTTATCAGTTGGTGAAATTACTTTAAAAGAATATAATAAAGTCCTTGCCAAAGAATTTTTGGTTGGTGCTTTAATTGGTTTAGTTTTAGCAATTATTAATTTTGCTCGTTTGATTATATATTTTGCTATTATTAAACCTGATTTACAACAATACAATGTTTTATACGAAAATATTACTAATAATCCACATACTAAAATGATTGTAGGAGCAATTGTTTCAGCTGGATCATCAGTCGCTTTGTTTTTTGCTATTGTAATTTCTAAATTGCTTGGTGGAACACTGCCACTTTTAGCAACAAAATTAAAAATTGATCCTACAATTATGTCAACACCAATTTTATCGACATTACTTGATATGGTTACAACTATTATCCTATTTGGTTTTGGTATTATATTTTTATTAATTATTGTTGATAAAGTTAAAGTCGAACAACTAAATAATATCCGCGAGCATATTCATGCTATTAAAAATGTTAATTCATGATTACCTAATCAAAATTTTTTATTAAATAATTTGAATTATTCAAAACATTTTGCTTAATTTTTTTATAGAAATAATTATTTATAAAATGTTATAATTTAAAAGCAATATGCCTAAGAAAGTAACTGTTTATTAATTAAACTTAATTTGTTACCGAGGTTTATATTAAACAAAAATGCATGTTGCAAACACATATTCTAAATAAGAGGTATAAATATGGCAAATGTTAGACCAAGTGTTGTTTTTAAACAACAAGAAGTCAACCATATGGCAGATATACTAAAAAATTCAAAATCTTTTATTGTTTTTGAATATCATGGATTAACTGCTGCTAACATTTTAGCGTTAAGAAATGTTTTACATTCATCAAATTCTAAATTATATGTTTTAAAAAATAATATTACAGCACGTGCTTTTGAAAAAGCTGGTGTTAGTGGATTTGAAAATCAATTAACAGGACCTAACGCAGTTGCTGTTGCAATGGACGATGAAATTGCTGCGATTAAAGCAGTAAATGATGTAGCTAAAGAATTTGATTTTGTCAAAATTAAAGGTGCATATCTTGAAAACAAATTTGCAGATACTCACAAAATCGATCAACTTGCTGCAATTCCTGGCCGTGAAGGTCTATACTCAATGCTACTTTCTTGCTTCACAGCACCATTACGTAATGTGATGTATGGTTTAAAAGCTGTAGCTGAGCAAAAAGGTGAATAAAATAATATTATTGGAGAATTTAATATGTCAAAATTAACAATTGAACAATTTATTGCTGCGATTAAAGAAATGTCAATGCTAGAATTAAACGATTTGGTTAAAGCTATCGAAACTGAATTTGGTGTGTCTGCTGCTGCTCCTGTTGCCGCTGCTGCTGCTCCTGTTGCCGCTGAAGCTCCAACTGAAGTAACTATTAAATTAGTTGAAGCTGGTACTAATAAAGTTGGTGTAATTAAATTAATTCGTGAGATTACAGGATTAGGATTAATGGAAGCTAAAACTGCTGCTGAAACTGCAGGCAGTGTAATTAAAGAAGATGTTAAAACAGAAGAAGCTAACGAAATCAAAAAGAAATTTGATGAACTTGGTGCAAAAGTACAACTTGTTTAATTTTCTCTTTTTTATAAATTGTTATTAATTAAAAACCGGCATCTTTTATAAAGGATACCGGTTTTTAATTCTATTTGTTTTTGTTCTAATCAAATTTAATTAGATATTTTATTAAGTTAAATTATAAAGTTATTATAGTTATAAATGATGATAAAAAAACATTAAGCGTTATACTAATTCATTTTTTATCTTGAATAGACACATACTTAATGTTTTATTTTTAATTATAATTGTGGAATCTTAATTCCATGTTTTGCATAGAATTCTTTTAGACCGTTGTACGTATTTTCAGAATATCCACCATCTTTTACTTTTGCTCCTGCATAAGATAAAAGCAACCCAATTAATGCTTGTTTTTCATCATTTGATTTAGTATTATCCCATATTTCTTTAAATTTCTTTGAAGATGTGTTAGAAATATATGTAGATTTGGCAACAGGATAGAAATAATATGTACCTTTAGCTGATTTATAAAAAGTTCCGCCATTTACTCTTGGATCAATCGTTTTATTATTTAATAAAGATGCTTCATTATCTGTTTCTTTTTTAACATCTAAATAACCATCAACAACTGGTTTTTTATCAAGTGTTTTAAATAAATCATCATTAGTGATTTTATTAACTAATTCATCATATGTGCTTACTTTATCACTTTGATTTGTTTGTGCTAAATTAAATGCATCAATTACATAATGATAAGCTGATGGTGAAATACCAACTACACCATCTGATAAATTACCAACAGTATTAACTCCTAATTTATATTTATCTTTTGGATCAATTTCGCTACCAATAACAATCTGGTCAGATTTTTTAGATAATGATTCTCCCTTAATTGCATTTTCTAATAATTTTATCGTTGCTGTGTCTAAACGTTTTGTAATTGAAAAACGAATAACACCGTTTTTATTTCCGCTTAGATGTGTGTTAATAAATTTATCTGTTTTTTTATTGATTGCTTGATCGTTTTCAATTTCTACGTCAACACCAATAACTCCTACATTGTGTGAAGTTGTTGCAATTGCTTCAGTAACAGCTATTCCAACTTGAGGAATAGCTACTGGTAAAATTAAATCAGCACCTTTAGTAATTAATTCCTGAATAATTTTTTTAGCATTAGCACTATCAGATTGGAAACCTCCAGCACTACTTTCTGAAGCATAAACTTGTTCAACATTAATCCATTTTTTTGTTTCTTGTGTTCCTTCTTGTTTAATGTTTTTATCTTTTAATTTTTCATTAGCTCATTTAACACCTAAATCAAAACCTGCTAAGTAATTGGTTGTATTTTTAGCATTTATACCTACATACCCACCTCAAGTTAATTTATTATCTGCACCAAAAACTGCTTGATTTGAGTTTAACATATAAGCTGCAGCAATACCTCCTAAAAAAGCGGCTTCATCAACTTTAAAATAAACATCAGCAACACGATCTGCTCCCTTATAAAACTCAGCTTTATTGTCATTATTATAAACTCCATCTAAAATTAAAGCGATTAAATTAGGATCAAATTTATCATTTACTAAAGCATTTTGTAATCTTTCAGGATGTTGATAACCAGGCATTAATAAAGCTCGTTTACCATCACTAGCCATACTTTTATATTTTGTTTGAAAACCTTCATCATCATCACTTGTTGGTGAAGTCCAATAAAATTGACTATAGTTTAATGTAGATTTTTGTGTACATGATGTTGCTACAGTAATAATTGTACCTAAAGCAAAAACGCTACCAATTGCTAAAAATAGAACTTTTTTATTTAACTTTTTCATTTTACTCCTTTATATTTTATTATTCACAATTTATCTTGAATAACTAAACAAACAATTTAAAATCGTTTATTTATTGTTAATAATATTAACCTAAAAAAGTTAAATTTTAAAATCATTTTTTTAAAAAAAATAAAGGAAAAAATCCTTTTTTTCTTATCTTGTCATAAAACTAATTTTTATAAAATTTGTTTAATGAAATTGATAATTTTACTAAAAGCCTTTATTTTAATTAGATTTAATTGAATGATAAAAAATAAATAATTAAAAAAGTAATCAAATTAAAACCCTTACTTCATCATAAAAAATAATTTATCATTTCTTTAAAAAAATATGAAATTAATGGTTAGTCTATAATAAAAAAATGTGCAAATAAGCACATTTTAATTTATCTTTTTGTTTTATCAAATGGGATTCCCACATTCTTTGGTGCCACACTATATTTACTAAATGAAATTAAAGCAATTAAAGTGATTACATAGGGGATTAATTTACCAATTTCTTGAGGTATTTTATCAATTTGTGAAAAGGTTTTTGTAATAACTGCAAAAGCAATTGAAACAACTGCAATTAGTGGAATTCTTCAAGCTCCAGCAATCAAAATGGCTAAAGCTAAGAACCCGATCCCACCAACCTCGCCTGCAAATGGAGAACGTGATGCATACATATATAATCCACCACCAATTCCAGCAATAAAACCAGAAATCGAAACAGCAATTCATTGGTATTTATAAACATTAATGCCTTGTGCATCTGCTGCATTTGGGTTTTCACCAATTGCATATAATCTTAAACCAAATGGAGTTTTTTTCATCATTATCATTAAACCAATGGTAATTAAAACAACAGCAATTAAAATTAAAATACTTCCAATATAAATAGTGTTGATTCCATCACCAATTTTCATTAATCCTAAATAATCAGAAGATAGACTTGTTTTGTCAGCAATTTTATCGCCCAAAGGATAAGTTACAAAGGTTGCTACTCCTGAAGCTAATAAATTAATAGCTGTTCCAGCAATAATTTGATTGGTTTTTAATTTTATAGTTAAAAAACCAAATAATAATGAAGTTGGAATAGTCAATATTCCAGCAATAATTAAACATACAATAAATGTATATTCTGTTTCCGTTTTTGTTCCCATATATTTATAAATAATTGTTGAAAATAATGTAAAGACTAAGGCACCAATAATCATTTGACCATTAATTGAAATATTAGCAATCCCAACACGTTCACAAAAGAAACCACTTAACGCACCTAGAATTAAAATAGCAGCGTAAATAGAGGCATCGTTAATTAATGATAATGAAGGCATTAACTAATCACCTCTTTCATGTTTTGTTGATTAATAATTGCTTTTTGATAAGCAGTTGTTAATCAATCTTCTAATAATAATTTTTGTTCTTGATCTAGATTAATTTTATTAATATGTTGTTTAGTTTTTTCATAATGTTTTGTCAAGTATGATGATGAGCGTTGTTTTGTATTCTTTTTAATTTCATCATCTTGCGAAATATTTCTTAATTCTAATTTTGTAATTTCTTTCGTGTAATTTTCTAAATGTTTATTACGCGAAATAATAATTTTATTAAGAATTTTATCATTTGGATTTAGTAATAAAATAATCTTATCCATTTGTTCATCATGAACTAAATTCTTTGATTTTAAAGTTATTGACAATTGTTCTAATCGAGAATCTAATAAAGAATTAATGTATGTCATAATTTGATTTTTTAAATCAATCATGTGATCTAAATTATTCTCAAAACTTGCCTGTTTAAATTCACTAAACATTAATTGTGCTTGTTCATCTTTTAGAACTAAAGATAACTCATTAACTAACTCGGTTGTTAACACTGAATTGTTATTTTCATATTCTTGTAATAAATATTTTAGCTTATTATAATCAAACGATTTTGATTGAGCATATTTACTAATTTCTTTAGCCACTAAATTTTTAATTAAAAAATCTTCATTATGAATTTTGTCTTTAATACGACTTAGTTTTTTAGCGCTTATGTTTTCAAAATGTTTTAGTTTATTTTTAATTTTTCTTGTTGTGTTAATTTCTGGATAATAAACAAGTGTTGCTCTTTCAATAATTAAATGTTTTTTAAGTTGCATTGCATTATTTTTCAATTGTTTCAAATATTTATCATATTCAACTTTTAATCAAGCATTATATGTTGTTTTAATGTAACTAATAGTATATTCATATTGTTGGTTAACTTTGTTATATTGTTCATGATTTTTAAATTGATCTAATTCTTTACGTAATTTCTTTAACTCTTGTAAATATTGATTACGTTTTTTATTAATTTCACTATAGATTGCTTTATATTCAGAAATAATGTTATTGTTTTTATTTTCATAATCTCGATAATAATCCTTACCTTTATAAGTATAGATTAAATGATAAATTCAATAAACGGGACTTATTCGTTCAAATAAAATAAACATTGCTGCCCCTAACATTACAAATGAAATAATAATTCCAGATAAATCTTGTGGAAAACTTCCTCCTAAATTATCAGCAGATTGTTGTAATAATCCCATTAAGAATGAGACAGGAATTGTTGCTAAAGGATGTGCTAGAGAAATTAATCCCATCGCAATTCCATCATATCCTTGGGTTGGTAAGGCATCAGCAGCTTGACTAATAACAATCGCATTTGTTGTTGTTGAAGTATAATTAACCATTGCTAAAGCCCCAGCTAATGCACCAGAGATCGCAAAAGTTGCTAATGAAATAATTTTTGTTCGGTATCCAGCATATTTTGCTGCATCAAATGATTTACCAACAGATAAGACTTTGTGACCAAAAACTGTGTATTTTAAGATTACTCAAATAATAATAGATAAAGAAATAAAGATCAATAAAGCAAATAAATAACCATATCCAGATGCTAAATGAAATAAAGAATAATTATCAGCAAAAGGAATTGATCGTGATCCAACTTGATCTGGTGTTGGATTATACATTTTACTTTGTGCAGTAAAAACAACATAACGCGTAATATAGAACATTATTCAATTCAATAAAATTGAACTAACAACTTCATTAATCTTTAAAAATACTTTTAAAATTCCTATAAAAACTGCAAACATTGCTCCTGCTAACATTGCAATGATTAATAAGAAAAATTGACCTAAAACATTAGGCATATTAATATTGGCAATTTGCATGTTTTTTACGACTAATAACATAATCAAACCTGAACCTAACATTTGTCCAGAAATACCAATATTAAATAATCCCGCTTTAAAAGCGAAAATAAATGATAATGCGGCAATTCCTAAAATTGCTACTTTTGTTAAATAAATATCTGGCGAACCAATTCATTTAGTAAATAATCTTATAAAGAAATCACGTATGTTATAGCCTAATATTCCAACCAAAATAGCTGAAACTACTATCGCTACAAAAATGGCAAAAACTGTCGATAAGAATTTTTTAGTGATTGTTTTTCGCTCTTGACCTTTAAGAAAGCTATCGTAACTTAAAAGAGTTTTAAATTTCAACATACTTATATTTTTTCACCTGCCATTAATTCACCAATTTTTTGTCTTGTCATTGAAAAACGATCACCAACACTAATGAAATATCCATTATGAATTACAGCAATTGTATCAGCAAGTGATAAAATTTCGTCTAATTCGTAAGAAATTAATAAAATAGCATTACCTTTTCGTTTCTCTTCTAATGTTTGTTCATGAATAAATTCAATCGCACCTAAATCTAAACCTCTTGTTGGTTGAACCAATAAAATTAATTTATGAGATCGTTCAAATTCACGACCAATAATTAGTTTTTGTTGGTTTCCACCAGATAATCCTCGTGCATCTGATGTACCACGTGTTGTACCACGAACATCATATTTTTTAATAATATTAATTGCATGTTTTGAAATTTCATTATTATTAAAAAATCCTAAATTACTATATGGTTTATCATCAACTTGTTGTAAGATCGCATTCATAGCAATCGTGTCATCTAAAACTAATCCATGTTTATGACGATCTTCTGGTACATGTGATAATCCTAACTGATAACGTTTTCTAATGCTTGCATGTTCAATTTCTTGATTATCAAGCATAATTTTTGCTTCCTTATTTCCTTTTAATAAGCCAGAGATTATTTGCGCTAATTCACTTTGACCATTACCTTCAACACCGGCAATAGCAAAAATTTCACCCTTGCGGATACTAAAATTAATAAAATTATTATTTTTAGTACTTAACAATGAAACATCATCACCTTTTGGTGATGATTTAACTTTCACAATATCTTTTTGTTTTTTAACAATTGATTGAATATCTAAATTTTGAACTTCGAAAACTTTTTCATTACTTGTGATTGGATCGATATTTTTAACTTCAACCAATTTTCGACCAACCATTAATTCAGCCATTTCAGCTATTGTTTTTTGTTTAACATCAAAACTATCAATATAATGTCCGCGACGAATAACTGTTGCAGAATCAGCAACTTCTTTAATTTCATTTAGTTTATGCGTAATAATAATGATTGTCTTGCCTGCTGCTTTAAAATCTTTTAGCATTTGTAAAAATGCTTGAATTTCATCTTCACTCAAAACAGCAGTTGGTTCATCAAAAATTAAAATATCAATATCACGATATAATAATTTTAAAATTTCCGTTTTTTGTTGTTGACCAACTGTAATTTTTGAAATTTTACTTTTTAAATTGATATTTAATCCATATTCTTGAATGATTTTTTTAAGTTCTTTAGTAATTTTTTTACGGTTAATGATACCTAGTTGCGTTGCACCTTCAGAACCTAAAATAACATTATCTAAAACACTTAAGGTGTCAATTAATTTAAAATGTTGATGTACCATTCCAATTCCGGTTTTTGAAGCATCTTTAGCTGAACTAAAATTGACAATTCGACCATTAATTTTAATTGATCCAGAATCTTGTTTATAGATCCCAAATAAAATTGACATTAAGGTTGATTTACCTGCTCCATTTTCACCAATAATTGCATGAATTTCATTTTTTTTAACAAAAAGATTAACATCTTTATTAGCAATAACAGTCCCGTTCAGGAAAGTTTTTGTAATCTCGTGCATTTCTATGGCGTAAGGACATTGATTTGTCTTAATTTCCATATGATCACTTTCTATAATTTTTTATATTTATTTCAAATTAGATACTTTTAAAGTATTAAATTTTTGGAATGATGATTTTTTTAGATTTGTAGAATGCTGCTAATCCATTATATGCAGATTCTGAAAAAGATTTATCTTTTAGAACAGCATTATTTTGACCTAAAACAACTTTTGCTAATTTTTCTTTAGCTTCTGGTGTTGTGGCAGCATCTCAATATTTTTTAAATTCTTGTGATGCACTTCGTTTCTTTGGATCCTCTTCTAAATATGAAGAACCTGTTAATGAATATGGATATAATTTTTTATCTGATTCATTTATATAAACTTCACCACCAAGTAATTTTTGTAATTCACTAAATGATTTATTTTTTTCACTATCTTTTAAAACTCATCCATCGCTTGTTTTTGTAGCAAGATCTTCTAATTTTGTAGTTCCTTTTGTTGAAAGAAGTTTAAATAATTCTTCATTTTGAATAACATTAACAATTTGAGCATATGTAATTGCTTTTAAACCAGCTAGTTTAGCCGCTTGTTCTAAATAAACACGACCTGCATTTGATAATTCAACAAGCGGAGTATCATCTACATATGTGCTCTTATCTAAACTTGCTTCAGTATGTGTTCCTAATTTATAAGCATCTTTATTAATATCATTAGTTAGTTGTGCACCTTTTGAAGCATTTTCAATTGCTCCCTTCATTGCTAAATCAACACGTTTAACAATTGAGAATAAAATTGTTTTTCCATCATTAACAGTTTTGTTTGTAATTCTTTTACGGTTTGTTACATCATCTAATTCTTGTGCAGTATCAACACCAATAATTACACTTGGATCTGTTGCATTTGAAACAATTGAAGTTGCAAGGTTTGTTTGTGGACCAGCTACAGGTAAAATAACATCAGCTTTTTTTGTAATTAAATCATTGATAATGTTTGTTGCACCTTCTTCATCTGGTTTAAATGAACCAGCAACGTATTTGCTAGCAAATACTTGTTCAACATTCATTCATTTTTTCTTGCTTCCATTAGCATCTTCTTGATTAATTTCTTTATCTTTTAACTTTTCATTTGCTCATTGAACACCTAATTTAAAACCTTGGATAAAAGTTGAAGTTGATGTAAAATGTAATCCAACATATCCACCTCAAGTTAGTTTTCCATCTGCTGCAAAAGTTTTTTGGTTAGCATTTAAATAGTACGCCGCAGCAATACCTGTTAAAAATGCTGCTTGATCTACACGATATGAGACAGACGCAATACGGTCTTTACCCACTGTTCCACTAAATTTATCATCAATTAAAACTGCACTTAAATCTTTTTTATCGCTGCTTGAACCAAAATATTCTTTAATTGGAATTGAGTGACTAAATGAAATCAAGCCTAACATACGAGCACCATCATCGTATAATGAATTATATGTTCTGTGATATCCTAAACTATCATCTCCAGCTGGTTTTGCATAAAAATCTTCTAAAGACAATTTAGAAATATAATTTCCATCTTTATTTGTTTTCCCATCATCTTTATTACTATCATTACATGATGCAGCAATAGCTGCAACTGAAGTTAATGTAACCACTCCAGCAAGTGATGCAAACAAGATTTTTTTATTGATTTTGCTTTTTTTCATAAAATTTATTTTTTATCCTTTTTGACATGAAAAATCTAAAATAACTAAACAACACAATTTTTAAGATTGTGTAGTATCATTATACAAAAAATAAACTTGTATATATAAGTTAAACACTTAATTTTTGTTTTTTGTATAAAAAAAAGCGTTTTTTTAATTAAAACACTTTTTTATTTTTTAAAATTTAGAATTCTTAATGAATTTTCTAATAAGATTTTTTGCATTTTTTCATGTGTAATGCCTAATAAGTTGGCAATAAATGCTGATGTATATAATACATAATAAGGATAGTTAGTTTGCCCTCGTTTAGGAACTGGTGTTAAATATGGGGCGTCTGTTTCTACTAGAATCTTATCAAGTGGGGTTATTTTAATCGCCTCATACAGTTCATGTAATTGATTATTTTTTGTTGGTTTAAAAGTAATTACACCAGGATATGAAATATAATAACCCATTTCAATATATTGTTGTGCATACTCTTTTTTATCTGTAAAACAATGGATTATTACATCTTGAACAGCTAATTCTTTTAAGATGGCAATTGCGTCATCGTGTGCATTACGGATGTGTAACATTAATGGTTTATGATACTTTTTAGCCAATTCAATATGTTTTTTAAACCAATGTTTTTGAAAATCATGATCATAATTTTGTTCATGATAATAGTCTAAACCACACTCACCAATACAACTTATGTAATCAATATTTTCTGCTATCAATTTTTCTAATGCAACAAAATCATTTTCTATATTATGTAAATCATTAGGATGGATTGCAACACTACAAGTCAAAAATTTAAATTTTTTAGCTTGTTGAATGGCTGTCTGTGAAGTTTTTAAATCTACTCCAACAATATTTAATCCAATACCTTGTTCATAACATTTAAAGATAATGTCATCAAATTCTTCAATTAATGGTTCAATATTAGGATGAGTATGAGTATCAATAATTTTTATTTTTTCTAACATATTATTTACCTATACAAAAATTTTTAAATAAATCATCTAAAAAATCATATTCGCTACTTATGCCTAAGAGTTCATTTAAACGTAAATTGCAAAATTCTAAATCAGCGACAATTAAATCAAGTGTATCCCCTTTTTCTAAATTAAGAATTGCGTTATTAATTAAATAATGAACATTTTCTAAAATACCAATTTGACGTTGGGATTGTAAAACATCAATGTTAGCATTGTCAAACTCTTGAACATAAAAAAGTTTTTTAATTTCATCAATCAATGACTGAATATCATTATTTTTTGCATTAATATATATTTGTTTATCATCGTATTGATCAACTAAATCTTTTTTTGTATAAACTAATAAATGTTTTTGTTTTTTAATTAAATCATATAACTCCAAATCTTGTTGTTCGTTTGCAGGTATTAAATATAAAATTAAATCAACTTTATCAATTAATGCTTTGGCTTTATTAATTCCTAAATTTTCAACAAAATCATTTGTTAAATGAATTCCTGCAGTATCTAAGATATTTAAAGTAATATTATCAATATTAATTGAGGATTCAATAACATCACGTGTTGTCCCAGGAATATCTGTAACAATCGCTTTTTGTTCATTGCATAAAGCGTTTAATAGAGTTGATTTACCTACATTTGGTTTTCCAATTATTAACACCCTAATGCCTTTATTGATTGGTAAAAATTTTTTTGATTGATCAATAATTTTTGTAATTTTTTTATCTAAAGATAATAAACGTTGTTTTAAAATAATAGCATCAACTTGTTCAACATCATCGTATTCAGGATAATCAATATTAACTTCAATTTGCCCAATTATCATAAATAACTCATGCCTAAATTCAGCAATTGATTGCGAAACACGTCCTAATAGAGCGCCAATTACACCTTTAACGCTTAATTCATTTTTAGCATTAACCAAATTGTTAATTGCTTCAATTTTTGATAAATCCATTTTTTTATTTAATAAAGCACGGCGAGAAAATTCTCCACGTTGTGCTGGTTGACAACCATATTTAATTAATGTTTTAATAATTAAATTAGCAACCACTATTCCTCCGTGGCAATTAATTTCTACTAAATCTTCTCCAGTAAATGTTTTTGGAGCTACAAATGTATTTATTAAAACTTCATCTAAAATTTGATTATCATCTTTTAGAATTGTATACCAAATTTTAAAAGTTTCTTTTTTTATTTTTATTGTTGAAATTTTATTAATTATTTCAAAAGCTTTTGGACCACTAACCCGAATAATGTGAATGGCACAATTCATAGGCGCGGTTGCTAAAGCAACAATTGTTGACATTGTTAATCACCTTTATTTTTATTAATAATTGTATAAATAATAGTAAACAAATTATTTTTATTAATATTAATTTTTAAATTCGCACGCATTAAATCCAAAATAATTTCTTTGCTATTTACATTATTAATTCTAATGAAGATTAATTTTAATAGATAATGAATTTGTAAGGTAGATAAATTTTTAAATATTTCTTTTAAATCATTTAATATTTCAAAACTTCTTAAATCACTTAATTTTCCATAATAAGTTAAAACATCATTGATTTCCTTGTTTTCTAATAAAATTTTTAGCTCATCCAAATCATCAAACATTAAGTCGCATTCCACGAGATCAATTGGTTGATTAATTTGGTTAATTAATTTTTTATATAAATCATGATTAGTAGGTAAATAAAAAAGCTGACAACGACTTTTAATAGTTTTTAAAACTTGATTTAAATTTTTAGTGCTAAAAATTGCAATTGTATTTTTTGGCGGTTCTTCAATAAATTTTAATAATGCATTTAAAACATATTTTGATGCTAAGTCAATATTTTTAATAAGATAAAATTTTAAATTAATATCTTCTACTCCATCACATAAAAAAGTGTTTTGTAAATCAATTACATCGCTTTTTTTCATTGATAGTCCATCATAAATTTTTAAATCAAAGTATTGTTTATTTTTAACTTTAGTAACATAAAATGATTGTTCTTTATGATTTTTCTCATAAATAAAAGCTAACATAATTTCTTCAATACCTGATTCTAGTGAGGTTTTAGGTGATTGAATTAATAAATTAGCAAAACTGTATTTCATTTATATATATTTATGTTCTTTAAAAATTTTTAATAATTGTTCTTTAACCTCACATAATACTTCATTTTCATTTTTATTGGCATCAATAAAAATAATTTTTTTAGGTTGATTATAAACTTTAAAAACACTAGGGTATTGTTTTAATAACTTTTCATAAAATTGTTTATTTTGTGAATCGAATGCATTTGTATTGTCAAAACGATTTTTCATTCGATTTAATGCATTATTAACATTAACATCAAAATAAAAAACATAATCTATTTCTAATTCACCAATTGCTTGTTGATTAATTTGGTAAATAACATCTAAACTTTGATTTTGAACAATACCTTGATAAATATATGAAGAATGAACAAAACGATCTGAAATTACAATATGATCTTTTTCTAAATGAGGATTGATTGTTTTTTTTACGTGTTCAGCACGACTGCTTGCATATAAATAAGCTAGTGTTAACGGATCAAAAACTTCTAAATTTTTTAAAAAAAACTCACGTATCATCTCTGCTGCATTATTGTTTTTTCCACCAGGTTCACGCGTTAAAAAAATTTTATTAACTAGTTTAGGTTCTTTTAAAACCTCTAAAAGTTGTTTTAAGATTGAAGTTTTCCCTGCACCATCAATTCCTTCAAAAACAATAAATAATCCTTTTTTAAGCGGTTTTTTTTCATTACTATTTTTCGTTAATATCATTGTTATCATTTTCCTCTTGTTCTACAAATCTATCCTCTAAAAAACTTAGTAATATATTAGTAAATAATTTTGGTTCTTCAATAAAAGGAATATGACCAGAATTATTAAAAATATATTGATTTACTTTATTGTTGTTTTTAAACACTTTAATAGCTAAATTAAAATCAATTAATTGATCATGTCTTGAAACCATAATTAATGTTGGCATTATTAAATTACGCTGTGCCATTTTTAATTCATGATTGATTTTAATATTTAATAACGATAAACATAAAAATAAGACATCGCGTGCAATTCGTGGATATTCTCTTGAAATTGACTCCCAACGAGTTTCTATTAAGGTTTTATAATTATTACGATAATCAATATATAATTTTTTTAAAACGACATCTGTTTTAAATTTTAAAAATGGATTGGGAAATAAAACCCAAGCATTAAATAATTTTTTATTTAAAAACGGATTTAAATATTGATAATGATATGGACAAACAAGAATTAATTTTTCAATGCGTTGGGGGATAATAAAACTAACCAAACTAGCAATCCCCGCTCCTAATGAATGACCTAACAAATGAAATTCTTTTAAATCAATTTCATTAATTCAATTAATAAATAACTCAGCAAAAACTTTAATATTTAAATCTTTTTTGTTATTAATAGGTGTTAAACCATGTGCAGGTAATCCAACAAAATAACAATCATAATCAGATAAATCTTTATTAATTAAAAAAAAATAATGTGGAGATGCATCAAGGCCGTGTATAAAAACAATCGAACCTTTTTTTAGTTCGCTTTTAGATGGCTCAAAATAAAAATTAAGTGTGTTAGTCTTAATTAATTCCATAATCATTACTTTCTTTTTTTAATTTTAAATAAAAAAATAATCAACTTATTTAATAGTTAATATTTTATTATAAAAAACCAATCTTTAATGAGATAAAGTATAAATTTAATTTTTCAATAAAATAACTTGATATAAAAAATATGGTCCTGAAATAATTGGCACTAATAAAATAGTCTGGTCAGTTGATAAATTTAAAATTAAAACAACAAAAATATAACTAATCAATAATACAATGCCGCCTATTAATCCAGCATTAATAATGCCATTAGAAATATTGTTTTTTACATAATTGAAAGAAAAATTAGCTGCAACAATCCCAACAAAAATAACATTACCACTTAAACTATAACTTACCGATACTAATAAGCTAATGCAAATAAAAGTTTGAATTGTCAAGGACTTATTATTAATGCCTAACTGTCCAGATATTTGTTGATTTGTTACCATAATCTTAAATTTAGTTGCATTTAACATTAACCAAATAACACTAATAGTAAGCATTCCAAAACAAAAATAAAAACAAAAATCGCTGCGTGATTCAATATTACCTAATAAAATTGTTTTTAAATACATACTTGATTCTTTACTCATTAAAATTCTTAATGACTGAGCAATAACAATTGTAATCAAATTAATAATAATACCTGAAACAATAATTCTTTTAAAAATGTATCCAGTAGAAGAATTGCATAAAAAATGAATAAAAAAACAAATCATTGTTGACCCAATTAAATAAATAAATGGTAGAACATATTCCATTCGTTTTTGGATTTGCAATTGCCCAAAATCAAATATTAAAAAAAGTATTGTTAAAATCACTAAGTTAATATTGCCCATTCCTAATAAAGAACTATCTGCTAAACGATTTTTTGTTAATCTTTGAAGAATATAACCACTAATTCCTAATGCAATTCCAGAAAACAATAATTGTGTGATAATTTTTCAATCGCTCAAATAACTAAAAACATTTATAAAACGCGAGGCTCCAGCTACAGCAATCAGCGTTAAAATAATTGTTATTATTAATAAACTTAATGAAAGAAAAAATTTGTTCTTTTTAAAACGAAAAAATAATTTTTGTTCAAATTGGTGTTTTAAATTTTGATCATGCTTAATTTTTATACTCATTTAATTTTTATTCACTTTCCATCCTAATTCTCATTTTAGCAAAACAATCATTGGAATCGCAAAAACTGTTGCTACTAAAAAAACAATATTAAATTTTGAAAAAAATTCTACAATTATTCCAAGTAATTCTAATAAAAAAGTAGTTAGAAAAATGGCGATAATCATTTGAAATAAATAATTAGTTCTTTTAAAAAGTAAACGAACTAAATGGGGCATAACAATTGCAATTAATGAAAGACCGCCAATTAAAAACGTAGTTGAAATGGCAATAAAAATTGAACAAGTTGAAGTGATTCAAAACACTTTATTAACATCGATTCCTAGTGATTTAGCTAAATAATGATCCGTATCTAAAATTTGGATTTTTTTAGCATAACATAACAAAATAATCGTACTTATAAACACTATTGGAGTGCCAATATAAAAGCGCTCAATAGAATTGTAAACACCAATACTACCTAATAATTGAATGGGGGAAGCGACAATTGATGAATTAAAATTAGCAATTAAAATATTAACAGCTGTGATAATGGCACCTAATGAAAAACCAAAAATAATAGGTTTAAAAGTTTTCTTATGTGGTGAAATTCTAATAAATAAAAAATTGATTCCTAAAGCAAATAAGCTAAAAATAATTCCAAAAACATAGCCAATAAAAATATTAGAAAAACTTTTGGTAATTAACTGACTCAAAATATTACCTAAGGCTGCTAATGGAAAAATTCCTAATGTTGATGGACCTGCCAAACCATTACGCGTTGTAGATTGCAAACTAAATCCACTGCAAACCAATCCTGCACCACAAACTAATAAAACAATTGGAGAATAAAAAAACAATAAAAAAACCTCTTCAAATGAAGCATTTTGAAAGTAAAACGATTCTCAAATAAATGTTTTGTTAGTATAAATAATGTTAAATATAAAAATAAAAGAAGTTAACAAAACCAAAACAACAAAAACACTTCATTTATATATTAAATATTTTTTAAAATTAAGTTTATTTTCAAATTTCAATTTTATTTTCATAATATATTATTATAAATTAAAAATCTGATTTAGCCGTGTTGATTCTTGTTTTAAATTGGGTTTTGGGATCATTAATTCCTTGTGTACTACTAATTATTCAAGATCCTGTATTTTTAATCATAATTAATTTATTCATTGAGTTTAATAAATGGGATGGGCCCATATAAATTGCAAAATTTTTATCAACAAATTCCGCTTTTTCACTATCATTAGGAGCCTTATTAATTTTTTCTAATTTATCATAATTAATCAACTCTTTTAAATAATTACCAACACCTTGGGTTTTTAAATTAATATTATTAAAACTTTCATAATCAACTATTGCATCAGGTTTTAAACCGAATGAAATTAAATGATCAATACTATCTGCTCATGTCCCAACAATTTTATAACCATAAGTTAGCTTAAAATTTTTATTTATATAATGAGGTTTAATTATTAAATTACGCTCTTTAATAGTTGGTTTGTGATCAAATAAATTTATTAATTCTTCTTTATTAAATGGGAGATAACTTCATTGTTTTTTTATTGTTTTTTTATTTGTGAAAATTTTTAATAATTCTTTAATAATTAGTGATTGCCCAACCATTGTAAAAGAACCTCATCAAAACAATGAACGATCAATAAAAATAATTTTTGAACGAGAATTGCGTAAGAATTTATGAGCATAAAAATTTTTGTTTGATAAAATTTCTTTAAATAAATCTTCACGTTTTTGACCATCTGTTTTGTATTTATTATTAGTAAATAAATTAGAATCATACATATAAAAAACATAATCAGCTGTTTTATAAAATGAAGATGTTTGTTTTAAGACTGATTCGAGCGGGTTTGTTCTTAATGCATTATAACCAATTGATTGATAACGATCAATAATATCACTAATATCATTATTAATTGGTTTTGGAAAATTCAATCCCAATCCTGGAACTTCTGTATAAGGATTAGAATAGATTAGTGGAAAATCTGAAGGTGTTAGAATAGAAAATTTATTATAATTTTCTTTATTATTAACTAATTCATTACGATTTTCTATAACTAAAACAGTTGCTTTATGATTGCGCATTTTTTGGATGCTATCGAGCATTGCTACATTATTAATATCATTTTTTAATAAACGTGCGATCTTTAGACTTCATTCAGTAAATGAATGTACATTTTGCATTGGTTTTGGTAGACGTTTTTTTAATTCATTAAAATTTAAAACATTATCTAATTGGCGAGCAAATTCTAAATGAATTTTATAAGGATCACGAAAAAAATTATTCAATCTCCAATTTTTATTTTTGAATAATCAATTAGTATCATACGGTTTTTGTTGTTGAAGATTTTTACATGAACTTAATGTTGTAAAAATAATACTTAATCCAAGCATTGAAATTACATTAATTAATCTAAATTTAAAACTAATTTTCATTATTAAAACCATTTTATTATTTATTTTAATAAAAATTAAGCTCTATTTCAATGTTTTCAACCCAAATAAGCTAAAACAGGTGCTCCTATAAAACTCATTCAAATCGATGAATTAATATAAATTGTCTCACGATATAGTATTAAACCTAAAGATGTTATCATTGCACCTAATAATCCTGATAAGGGCATAACATAACGATAATCTCTAATTGAAAAAATTTTACGCACGATATGGGGACCAAATAATCCAACAAATGCAATATTACCAACTAATAAAATAGTTGGTGAAACTAAAAAAATAGTTGAAATAATAGCTAAAATTTTAATAATAATAATTGACGATCCTAAGTTTTTAGCTTTTTCATCACCAATTTCTAATAAGGTTAATTTGTAAGCTAGAAAACAAGCTAATATTACACCAATTAGAATTAAAATTGCACTAACTCATAATGTTAAAAAAGGATCAATATGAGTTGTATAAATATTTTCACTACCCCCTAAAACATATTTAAAATTAATACTTGAACCTTTAGCACTTTGAATACGAATAAAATAAGAAATTGTATTAAAAAAAACTCCTATAGCTAATCCTGCTAAAGTAATTTTAATATATGATAATCTGCTTCTTGTTGCAAAAACTAGAATCAATAAAATAATGCTAGCAATTATTCCACCAATAATGGCAAAACCAAAAAGTTCTCCAAAAGAAATCATGTTATATGAGAATGCAATTACAATCATAAATACACTTGATTGTACAATACCTAATGTACTTGAATCAGCTAATGGATTTTTAGTTAGTGCTTGTGTCACGCATCCAGCAATTGCCAAACTAATACCTGATAAAATTGAAGAAATGCTTGCTCAAAATGTAGCTTGTAAATTTAAATATTTAATATTTGTTGGATCTAAAAAATGTCCATTAAAAATTAATTTAAGGGACTCGTGTAAGGGAAAGTAAACATATTTATTTAATTGTTGATCATAAAAATTAGCATATGAAATATTTCATACTCAAATACTAAAAATAGCAATAATTAAAAAAACTGTTGCTCCTATTAAAATGAGTGGCTTTAATTTTTTAACTTGTTGATTTTTAATTTGTTTAAATAGTTTAAAACGAAAGGTATAATTTTGCATTAATTGAATATTTTATTTTTAATTTTTAATATCTATTAAATATATCAAATTAGATATTTTTTTAAGCGACTCCCATTATATTTTGTATATAAATTATCTTATTTTTAAAATAATAGGAATAAAAACACAACCGCATCGGTTGTGTTTTCCTCTTAAACATTAAATTATTTTGTTTCTGCTTTAATAACGTTTGTTTTTATTTCATTGGTTGTTGAATCAACTGGTTTTTTGTTATTAAATTTATTGAATGTAGATTTATGATTTACATATTTTGGATTATAACCACGTTTGCTTGGGTCATAATTATTTTGTTTTCAATTTGATTTTGTTTCAGGTAAAACAATTTCGCTATCTGGTCGTCCAACAATTTTTGTTGGTTCACCGTGCAATTCAGCAATTGAATCTGCTAGCACACCTAAAATCAAAGTAATTGATTTAACTGAATAATTATTTACTGGAATAATGTAATCAATCAATTGTGGATCAGCATTCGTATTTGCTAAAGCAATTACTGGAATGTTTAATTTACGTGCTTCTTTAATAGCATTAACATCATTAACAGGATCTACTAGAATTAAAACATCTGGACGTTGTTTCATATTTTTAATCCCGCCATAGAATTTTTCTAATTTAGCAGTTTCTTTATTAATTGCAATTTGTTCTTTTTTAGAATATTTATTAATCTCTTCACTTTCTAATAAAGCTAAATTACTATTAAATTTTTTTAATGAACGTGAGATGTTAGTAAAGTTTGTTAAAGTTCCACCTAATCATCTTTGAGTTACATAAAATGAATTAGTACGTTCTGCTTCTGCTTTAATTAATTCTTTAACAACACGACCTCTTGTACCCACAAACATCACAGTGCCCCCGTTTTGAGAAACCTCTTGTAAGAATTTGTATGCACGATCTAAAAATAGAATTGTTTTTAAAATATCAATTACATGGTTATTTGAACGTTTAGCATGGATGTAACTAGCCATTTTAGGATTTCATTTCTTAGGATTTAACCCAATATGAGCACCTGATTCAGTTAATTTTACAATTGAAACTAAAGTTTTAAGATTTTTTATAGTTGAATTTGCATTGACTTTAAGCTGAATTGCATCAGCCGGTGATGTAGGTTTTTGTTCTACTTTTGCATTTTCAGTAGTAACTACTTCAACGTTAGCTACCGATTCAACTTTTTTCGTCGAATTTTCCATTTGAGACATGGAATCCACCTTTCGTAATTTTAAATATTTGTTTGTTATTTAAATCAAAAAAATAAAATAACTTATAAATTTTAACAAAAATATATTATTTTAAATAAATTATTTATTTTGATTAAATCCTTTAATCGTTTTTAAATATTCTAAAACTGCTTCTTGACTTCCATTGCCTTTTTTAATCAAAATCTTAAAAACCACCGCTGTTTTGTTATTAACATCAGGTTCAACACTTTGAATTTCATAAATAAAACCTGTTTGGAATGGTAATTTAAATAATGTTTTATAATTTTCAATAACTTCTTTTATTGTTTTTTGTTTTGTTTTTTCATCAATTAATTGTGGTTCAAGTTGATTTTTATCTTGTTTAATCGCTTCTAAATTAGCTAAATAAATCTTTTTATCAGCTTCAGAAATAAAACCATTAAGTGTTTTGTTATAAGTAATGCTTGCTAAATTTTCTCCATTACCTTTACTGATTTTAATTTCGATATCAATAGAATTAGCAGTTTTACTTTTAGTAATTTTTAGAATTTCATATTTTAAATCATTATTACTATTGTTAATATTTAAATAACTATTTTGTCATTTTAATGCTTCATCTACTGTTGTTTTACTAATGTTAATACTTGAATTAACTATGGGCATTAAATAAAGAGTATTATGGTTTTTTTCATCTTCTAAATATTTTTTATTTTCTAAAAATAAACGATATTGTTTAGCATTCTTAAAACCTTTTAAGATTTTTTTGTAAGTGTGCGTTTGACCTTTAAAATGATAACGAATTATTAGATCAATTACATCTTCATCTAACTCATTCAATCGTGCATCATATAATTCATATTTAATATCTTTATTTGGTGCCAAAAATTGAATATTAGTTTTAATAATCTTAATAAATTCTGAAGCTAAAATCTTTGAAAAATCATGTTGATCATAACCTTTAATGGTTGGATAAATTTCATTCTCTTGTTTTGATTTTATTGATGAGCTGCACGCAACACCACTTGTTGTTATCAAACCTAATAGTCCAACAATTAAACTACTAATGAAAACTGTTTTAATTTTTTTATTCACTGTTTCTTTACCCTTTAGTCTAAATTAATTTTAATTTTTTTAAATAATTGAGCCTGTTGTTCATTTTTACCTTTACTAATCATTATTTCCACAACTAATTCACGTTGTCTTTCATCTAATGGTTCAAAATAGTTAATGTCTATTATTTTAATATTAATATCTTTAAATTTTGAGTCTACTTTTAATTGAGGAATTAAATCCTCACCAAAATTAAAAGCTTTTTCACCTAATGTTTTAATTTCAACCAAACTTCTTTTTTGATATTCTGGTTTTATTCTAAGATTAAAAAAACCTATTTTTTGTTTTTGATCATAATCCCAAGCACTTTTAATAATATTTAAATTTTGTTGAAGGATTGTTTTATCTTCTTTTTGTTTAGGAGTTAAAAATGGTTGAAAATAAAAATCATAATTAATTGATTCTTTATATTTTTGAATTTTAGCATTAATCGTAACTTTAAGAATTTGTTCTGAATCCTTTTTAATATCAACAATTTTATACTGGACATTCTTTGGATTTCTTGATAAATCATAAACTAGTAGGTTATTTGATCCGTCTTTATTGATTTTTACTTTTTTTAAAACATCATCAATAGTATTGTTATCATTAGCAAATTCTTTTGCTAATGAAACTGTTGGACGATAAGTTAAAATAACATCCTGGTTAATTTTAAATTGCGAACAAGCGCTAACGATAAGGGGAATTGCCAAAATTGGCAATCCCAAAAAAGACATGATTATTTTTTTTAATTTTATTTTCATAATTTCTATAATTTTAACTTTAATGAAATTTTTTGAGTAATTGTATCTATTTTATCAATATAAACATTAATATTTTTATTAACATGCAAATTATCTTCTTCACAATAGTTTGAAATATGTAACAATGCATCATTTTTTAAACCAATATCAATAAAACTACCAAACTCTGTTTGGTTACGAATTGTTCCTTGTACTAACATCCCTATTTTCAAGTTATTAATATCAACCATTTGTGAACGTAAAATAGGAATATCATAATCATCACGAACATCTTGTAAAGGATTTTTTAAGTTATTAATAATGTTTTCAATAGTATAAATATCTGTTTTTAGAATAGTAGTTAATTCATTAGGATTTAATTGATTTAAAACTTGATAATTATTTATTAATTTATTAATTGGTAATTGTAAATATTTACATAATTCATTAACAACTAGATATGCTTCTGGATGAATACTTGTTCTATCTAAAAAATTATTAGCATTATTAATTCGTAAAAAACCAGCGGCTTGCTCAAAAACTTTATCGGTAATGTAAGGAATGGTTTTTAATTGTTCACGATTTTCAATAAATTGATGTTTTTTAACATATTCAAAAATTTTTTTTGCACTTCGCTTATTTAAACCTGATACTTTAGACAACAAAGGAATTGAAGCAGTATTAACATCAACACCAACTTGATTAACACAATAATCAATACAAAAATCAACTTTTTGTTGAAGAATGTTTTTATTAATATCGTGTTGATATTGACCTACACCAATACTTTTTGGATCGATTTTTATTAATTCACTTAAAGGATCAATGATTCTTCGTGCAATACTAATTGCCGAACGTTTTTCAACACTTAAGTTAGGAAATTCTTCACTTGCAATTGATGATGCTGAATAAATTGAAGCACCATCTTCACTAATTACACAATAATTAAGATTTAATTTGAATTCCTTAATTAAATCACTAATAAAAATTACACTTTCATTTGATGCTGTTCCATTGCCAATTGCTATTGTATTAACTTCGTATTTATTAATTAAGGATATTAACGTATTTTTAGCTTGTGTAATTAATATCTGTGGTTTGTGAGGGTAAATAATATCAGTATGTAATAATTGATTGTTTTTATTAACAATTGCTAATTTGCAACCATGCACATATCCTGGATCAAATCCTAAAACAACATGGCTTTTTAAAGGTTTTTGTAATAATAATTGTTGTAAATTATTACTAAAAATTTGTGCTGATTGTTGGTGAGCTTCCTCTAATTTTTCTTTGTAAACAGCATTTGAAACTGAAGGAATTAATAAACGATCAAAACCATCATTAACAGCTGATTTAATGTAGTCATAGCTGTTACTTTTGATTTTTCTAGTATATTTATTGACAGCAAAATCAATAAGAAATTCTTTTTTAAAATCAAGTTTAAATGCAATGATTTTTAATTCATTAGCTCGATCAATAGCCATTAATTGATATGCTTTTAAATATTTAATTGGGCAACTAAACTCATAATATAAAGCAAAGTTTTTAGTTGGGTCATTTGTTATTTTATTAATTCTTGTGATTAATTTAGCATGTTTGTTAATTGTTTCATAAAGCATTTCTCTTAAAGTTGTATCGTTAGCAACTTTTTGGGCAATAATATCACAAGCTCCAGCAATAGCTTCATCAACACTTAAAACTTGTTCATTTAAATATTGTTTTGCAACTTCTTTAATATTAGAATTAGGATTATTTTTTAATATTAATAATGCTAATGGTTCTAACCCTTTTTCAATGGCGATTGATGCTTTTGTTTTTTTATTTGAGGCATAAGGTTCATAAAGATTTTCAAGATCAACAAGCCGTTGACAATTATTAATTAATTCTAATAAGTCATTAGTTAATAAACCTTTTTGTTCTAAATTTTTAATAATTACTTCTTTACGTTTATTTAATTTTGTAAGATATTCATATTCATGTGCAATTGCTTGAATTTGAAATTCATCCATATTGTTAGTCAAATGTTTACGATATCTAGCAATGAATGCAATAGTATTATTATCAGCTAATAAATTCAAAACATTTGTTATATATTTTGCCTCAATTTTTAATTTTTGAACTAGTGTTTTAATAATTAAATCAATCATTTTGCACCCCTTTTAGAATTAATAAATTATAAACTTTTGATAGTCAAAATTAGTTATGTTAATTTAAATGCTTTATAATTACTAAGTAAAAGATAATTGGAGAAATTTTATGAATCTAAAAACAAAATTTTTTTTAAAAGTTATAAGTGTTATTGCTCCAATTGTTATAATTCCAACTATTTTAGCTAATTGTGCACATATAAATTCTAATGAACTTGATAATGCTAAAACAAACTTGAAAGGAAGTGTTGAAGTTGTTAATATTAATCCTTATAAAACAAAACAAATGTTAGCATCAAATATCAACAAAGAACAAATTAATTCTTATTTTATTTTTATTTTTAATTTAATTAAAACAAATCAAAAAATTGAAGAAAAAATTTTAGATAAAAATGATTATGAAATTCTTAATTGAGCTGCTAATGATAATGATGGAACGTTAGGTATTAACATTTACATTAAAACCACTAAACAAAGTTATTTAATTAATACTAAGCCTGTTTTTTTAACAGATAAACAAAACAATTATCTACAAGAATTAAAATATAAAACACCTGCTGTTTATAGTATTGATGAAATCTTAAAATTCTCAAACAACCCTTATAATTTAATTCACAATAATCCATACTATAGAGAACAATTATTGCGTGCAAAAATTTATTTTAATCAAAATGAAGCTAATGTTGATGATTCTAAACTTTATATGAACAAAATCGGTTATTCTGAATTTGGTAGTGATGTCCAAAAAAGATTAGAAAACGCCTTTAAAATTCGTTATGATGAGCAAAATATTTATCAAATTAATTCTCCGCAAATTTTAGCTAAAGAAACAAAAACGCTTACATCTTATATTGATAAAAAAACAAACAACAATTATAGTATTAATATAGAATTAATCAAAAAATTAATCCAAATTAATCCTTTTGGTAAACTCCCCAAAAATTTCGCTCAACTAATTAATTTAATTAAAAAAGAAGAATATCCTAAATTCTTGACAATTACTAAAAACGAATCTGTTGACAATATTGTTGTTAAAGATATTTATTATCGGATTATTGATCGTTATGCAAAATTAGAATTTATTTTAGAAATTTATAACAAAAAAACAAAACAAACCGTATATTTAAGTGCTAATTTTAATCAAAAGAATTCAGGATTATTAAAAAATGAAGATTATTTTCAATATATTTTTGATCGTACCATCTCACTTGATTTACTAACAACAAAAGATGGAAAAAATGTTGAATTAAATTCTGGAACTGGTTGAATTGTTGATCGTATTATTGACGATAGCTTACCTAAAAATAAAATTAAATTACTTATTGCAACTAATAATCATGTTATGGGCTGATCGAATTTAGCAATCTCAAAAGATAACAGAATGAAATCACGCTGGTTTAATAAACAAGAATATATAAATTATTTAGAAAATAACGCTGGTTTCATATCCTCAAACATTTATGAAGATAAAGACCGTTATCAATATTTACTATGGGGGACTGCGCCTCTAAAATCTCCAGTAAGTAATAAATATAATTCATTAAGTGGTATTAGTTTTTCAAATTTAGCAAAGGTTTATAATATTACAAATCAAAATTTCATTAATCGAGCATGATATATACCACAATTAAGTGCTAATGGTATAAAAATCAATGAAAATTTAAGAACATGATATCAAATTAATCAAGAAGATATTAAATCAATTAAAAATGGAACTTTAGACTTTGTTTTGGTCCCAATGGTTTTTGATATTGAAGATATTAAAGAAAAATTACCAAATTATTATAAAGTATTAAATACAAAAGATGAAGCAAATTGATATATTGGATTAGGCAATTCAAAAAAATACTTGCCACAATTACAACTATTTAGTGGAGGATATCCTGGTGATGTTAATCCTAATAGTTCAGCGATTGTTTCATGGCGCGGTTCTAAATCTTATGGTTCACTAATTCAAGCATTTGATCGTGAAATTAAAAATGAATCAATTTTAGATTATTATGGTCCAAAGCAAATTAATAATATTGATGGTTATCAAAAGGTTGGCGAAGGTTATTTAAATAAATTATTTAATGTTGGAACACGAGTTATTACCTCTGATGAAATAGGTGATTTGGGTTCTGGTTCATCTGGTTCAATGATTATTGATTCTAACTTTAATTTAGTAGGTATTCATTTTGCTTCTTTAAATTCGCGTGCTTATGGTGCGCCAAATGATTCAATGATTGGTAATTTATTTGTTGCTCAATCCCAAGACTTAAGTGGTGATATTGATGTAAGAGCTGCGGTGATAAAAAAACTAAAAGCTGAAAATATTTACACTTATAAACTAAACCCTAAAGTTAGTTCTTAATTTAAAATAATTTGTAAAAGAGAATGTTCTTTCATCTATACCAAATATTAGTAATTTATTAAAAGAATCATATGTTGAGCTTCTAGAAATAATTATAAAAGTGAGTTACGCAGATAGTGGTTCTGAAGTAGTAGGTTTATGCGAATACATAGTATTACCTAATCTTCTTTATATTAAAATTATTTTATACCTTAAAACCGAACTAAAATATTAAACCAAATTTAAAAAAGTTTTTAATTATAAAGGACAATACTACGATGGAAATGAAATGACAGAAATCTACAATTCATTACAAAAGGGAAACATTTACCCATAATAATGTTTTATGACAATTAATTAAAACTTTTTTGTTTGCTTTTATTTTAATAACTTGTTTTGTTTATATTTGATTATCATGGCCATTGTTGCTAAAATAAACGGAAATGCATCAGAATGATATCACGCACCAATCGCTTTAGCTTTCTTCTTACGGTTTTCATTAATTAATAAAATTTTTGCAATTATACTATCTTGTATTTTTTGCGTAAGCATTTATCGTTTTCGTAAAAAATTAGTACAATATGAAAATTTTAATGAAATAAAATCAATTAAAAATAATGCGATTCTTACTGTAATTTTTAGTGTTGTTGCAATACTTTCATTAACACTATTTATTAATTTAATGATTGTTTTTATTAATCAAAATTACTATTACATTAAACGTATTCATAATTTACTTATACCAATAAGAATTGATGAAATTAAAATTTTAGAATATAGTTACATTTATTTATTACGTTGTGGATTTTATATTTTATTAACAGTATTTATAGCAAAATTTATTCATGATAAAAGAAGTATGATAAAGGTTTTATCATTATATTTATTCGTTAATTTTATATTAATGATTATTGGAACTTCATTTACTTTTTTAGAACATACAAACTTTTATAATGGTATGTTAACTTATTGAACAACTTTTAGTTGTGGATTATTATATCCAGAAATTATTAATGATAATTTTCAGTGAATAAGTAGTTTTAATTACAATCTAAAAAATACTATTTATGAAAATAACTTTATTGCTTATTTACCTTATTTTTGCTCATTTATCGTTTTAATAATCTTAATTAAAAAAGTTAGTTGAAAATCAATTACTATTAGTTCAAATAGTTATTAAAATCATTTTGAGTCTAAGTATCTTTTTAAGTTAAGTTAAAAGTAGTGATAATATTTAAAAATCTACTTTACAAAACAAAGTAGATTAATAATTAGTTATCTTGATTTTTATTATATTTTCTTAAAGGACAATAATCATATTTTTTAGTATCTTTATCATTATAAGATTGACTTTCATATCCTAAATGATGATGATCAATAAGTAAAGGAAATAAAATATTATCGATTTTTGAGCATATTACTCTAACTGATCTTCCAAGGCCTAGTTGATAAAGTTCTAATTCTTCATTATCATTTTTCAATAAATTAGGATGGAGTTTTTTAATTAATTTAATATATTTACTAAATACATTATTTTGTTTAATAGGATGGCAATGATCGTTAATTCCTTTATTTTTTCAATTTTTATAAACATAGGGAAACAATGTATTAAGAATATAAATAATATTTTTTAAAAATTGGTTTTCATCCTTAGTATAAGTCGTTAAACTATTTACTCGTTCCTCTTTAACTCAATTTTCATAACGCAAAGAAATAACAATGTCATCATTATCTTTTGAAACATTGTTATTTTTAACTTTATTGCGAGGAGTTTTATTGTTATTTATTTTCTTAGACATTAATAATTTAATTATTCTTCATTTGTTTTATATAATAACTAAATATTTCTTTATCGCTTATTACTGCATTACAACTATCTAGCGGTCCAAACCCTTTGCGAGCTTTAATTCATGGGTCTTCTTGATGTGTAATACTTTCTAATTGATTAGCACTATAATGACCATATTCATCTCAAACTTGATTCAATATATCAATAGTATCTAAATTAAAATATTCTAGTTCATTAAAATCATTTATTTTAGGAATACTATTATATTTATGTTCTTTGAATTCATTATAAAGCATAGGAATAGCTGGTTCATGAACTCAAGCTTTGATTTTTTCATCAAATAATTTATTTTTTAAATCATCAACTTTTTCATTCATTAAAGTTAGGTATCATGAATATGCATAATACACTAATTTTTGAACTTTTTTGGGAGTCATTGATTCTTTTGTTAAAAATCAATTAGCAACAATTAATGGTGTTATTTCTTTCATTTCATGCACATCTTTCCTTATAAATATTATATTTAAAAAGTTAATAGTTTTACTTTTTTCTATTATTAAATTTTGATATTATATTGTTTAGTTCATTTTTATTTTCATCTAATTCGATTATTTTTTCTTTTAAAAATAAAAGTGCAAACTTGGAAAACTTATGTGTTTTATTGGTTTTTGATGTTGTAATTTCTAAATGATATTTTTTCGATTCCTTCATTTTAAAATATCAAATAAGTGCATATAATTTTTGAGGTGTTATATTAAGTTTTTCGGCTAATTGTTTTTGAATATAGGGATAAGTTTCATCTAAATTTTCTAATGGAACTGGAACTTCTTCTGCCAAATTTATATTTCCAGTAACTTTGATTACTGGTATTCCTTCATTTTCACTAAAACTACCTTCTTTTATAAATTTTGCTTTTTTTAGTAATTTTGCTATAAGATTTTTTTCTAAAACAACATCAACTCCATAAGGAGTGGACAATTTTCCGTTATTATAATCAACTATTCCTAAATTAGTAGCACCATTATTTCTAATAAATTCAAAAACCTTAAATAAGCTATCTCGTTCTTGCTTCATTCTACAATTAATAATTTGTTCCATTTCTGCATATTTTATTTTTTCAGTTCTCGCTCTATAACGATAAAATATATCACCATTAACAATTTTTTCTGAACTATTATTCTTTTGTGCTATCACTGGTTTTATATTTGATTCTTCGGTATAAATTCACCCAATAATTTTTTGAACAATGATAACATCCCCATTAGAATTAATTTCTTTTTCTTTTAAAATAAATGAGCCTAATTCTCAATTTATAGAAGGGGAAAATAGATTATTAATAGAATCTGTCAAATCTTCTTGTTGAATATTTTCAAAATTACTATTTTGTAACCCAATTATGGAACGAGGTGAATCTTTAATACCAAAAATAATATATCCACCACTGTTATTAGAAAATGCTGCCATAGTTTTAGCATATATTGCAATGTTGCCTTTATTAAATGATTGTTTAAATTCAACTTGAGTATTTTCCCTACTACGCAAATTTCCATTTTCCATACGAGCAGATAATATACTCTTTACTCTTTCTTCATGTTTTGATATAAGCATCTTAATTTACTTTAACCTCCATTAAAATTTAAAATAATATGTTATTTTATAATACTTATATAATTTTGCATTAAGGTTTTAAAAAAGTATGAATTTATATTGATTATGTTTTTATTTTTTGATAGCCTATAATAAGAGCGCACATTCTTTTGATAAAATCTTCATAATTATTTAATATTATAGAATTACCAAATAAATCATCGATTTTGTTTGAATAACTTAGTAAGTCTTCATCATTCATAATTTCTTTATAACATCTTATGTAATCTTTTTTATTTTTAATTTCCTCACTAATTTCTTTCAAACTAAGTAAAATGAAGAGCGTTTTATCTGGTGTATGAAAATAAACTTTAGGGTCATCATCTGATAATTTTAAATATTTTTCCAAATTGTGGTTTGAAATATTATCATATTTTTGTAATACACCATCACTTTTATAGTATGGAACTTTATCAAAAATTATAAAAATTTGAAAATAAGGAACTGAATTAGATCGTAAATTAGCTGTTTCGCCTAACATATTCTCAAAATAATTATTACTATTTTGAGAATAATTACTCATAACAAATTTAATTGCTAGTCCAGCAATTGATTTTCCATTATTTTTAATTGTTATATCAACTTTTTTGTTGTAATATCTACCATCAATACATTCTTCTTTATCATCTCTATAACCTTGGGATTTAATATTGTATTCTTTGCCTAACAATTCTTCTAAATCTTTTGCTATACTCCCATGTAATGTTTTTAATTTTTTAGTACTTCTAGACGTTCCAACACTCAAATAATTATCAAAAGATTCCTTTATAATATCTAAATCTAAAAATTTTTTGTTACTCAACATTTTATTAAATTTCCTCATTTTTTAATTTGTAATCCAAGTATTTTTTTACATCTTTCGATGAAAAAGTATAATAACCACCATTTTTATATTTTCCTAATAAAGAAATATAAAGGCCAAATTCGTTGTCATTCAATGCTTTCTTAATTTTGGGAAAATGTTTTTTGTCACTTACAACATATAATCCACTATAAACACCGGTTCCAATTGGAGCATTAACAATTTTTAAATCTGACGTAGTTTTTATTAAAGAATTTATTGCAATTTTATCCTTATAAGTATCTTTAATTCCTTGACTTCTACCAAATGCATATCAACAATTTTTTGAACTAATATCATGATTTTTTTTAGTAAATTATTTTTATTAAATTTTAAATAATTGTACATTTTTAAATCTTTTTTTAACACTAATTCATCAATTAAAAAATTTTCTTTATCGTATGGATAAAATATTTTTTTTAGTTCTCCTTTCGAAGCTTTAATGACAGGAATAATGAATTTTGATTCAAAGTCAAAATCATTAATAAATATTTTATCTGACAATGTTGCATAACCATTCTTTACTAATATGTTTGTGGTTTTATTTGAAAGTAAAACTTTTTTTAAAAAAATAAGTTTTTGTTTAGGTCCAAAATAATAATTATTTTTTATAAAATAATCGTTGTGGGATATTTTTTCAATAAAACAAGGTTTTAATTTTTTCTCATCAAATTCATAATATTCAACACATTCATTTTTTTTACTCTTATTTAAACAAATAATAGTTGTATAAACAGTAGCATTAAAAGCTTGAAAATGTTTTAAATCACAAACACTTTCGATTAATTTATATTCACTCAAAAACAAGCGCATATTTAATCCTGCTAAACTTGTAAAAAAAGAAGATGGTGTGATGTAGCATAAAATTCCATTTTTATTTAACATTTTTATACCAATTTCATAAAATATAATATACAAATCAGTCATTCCTTTATTACTAAATAAATAATTTTTAATTAAATTGGGTTCTTTAATCAAATTATGAATTCTAACATATGGAGGATTTCCAATAACAAAATCCATTTTAGAATCATATATATTTATTTTCATACTATCATCATTTACAAAGTTTCATAAAACTTTATTTAAACCATACTTTTTAACAATATCATCACACCTATGTCTACAAATTTCTAATTCATCATTATCAATTTCTATACCATGTATAAATGTTTCTAATTGTTTTTTTTAACTCAATTAAATCATTATTATTTATAAGATAATCTTTGCAATATCTATCAACTATATAAGTTAAAAATTGCCCATCTCCACAACTATTATCAATTACATGTTTTTTATTTATATTTCCATTAATATAATGCCCTTTATCAAGTATTATATTTACTAAATAATCAGGTGTATAAACTTTTCCATAGCGTTTAACCTTAGTCAATCTCAATAATTCTTTCATTTTTAAATTTAAAAACCTCTTTTAGACATTATATTAAAATTAATAAAAACGTTTTTATTTTATTGATAAGAGTTATCGATACCATAAAATAATTTCAACAATTTAATTAATATAGTTAAATATTTCATTATTTTTTATATTTGATGAAATGACAATATAAGGGTTTTGCTTTTTTATTATATATTTACTAAATTAATCACTTTTCTCTTTTAATATCAAATAAATATACTAATATAATTAATTTTATCAAAATCCAAAATGGGAGTCAAAATGGGGTTAAAATTAAACAATTGCTTTTAAAACAATTATTACTAAAAAACTAATAGTTTAAACTTTATTAATCACAATAATTTAGTATTTATCCAAAATTTTAATAACAATATTAGTAGTATAATGACTTGTAAATAATACAAGGAGATTTTGGTGTTGAATTTAAAACAATATTTAGGTGAAACTAATTTTTATGATAAGAAAGAAAAATTAGAAAAGAAAAAAATAAAATCATGACTAAAAAGTGTTTGTACATTTGCTAATGGTCAAGGTGGCAAATTAATATTTGGTGTAAATGAAAACAATAATGTTTTAGGATTAGAAAATTATTTAAATGATTCTGAATTTATTAGTGAAACTATTAAAACAAAAATTGATAATATTCCAGAATTTGATATTGAAATTAATGAATACGAAAATAAAATTATTCTAATACTAACAATTTTTCCAGGAAGAAATCCACCATATTTTCTAGTTGATAATGGTTCAAAAACTCCATATAAAAGAGTTGGTAATCAAAGTGTAATTGCTTCAATTAATGATTTATTAAATTGATGTTTAAGAAGTCAAAATCGCACCTATGATTCATTAGTTTCTAATAAATTACTAAAAGATGTTAGTTTTTTAATGTTAAAAAATGAATATCAAAAACATACAGAAAATTTATGAGAGGATAAATTTTTAAAATCCTTTGAATTAATTGATAACAATAATTACTTAACAAATGCTGGAGCTTTATTTGCTGATGGATATCAAGTTTATCAATCAGGAGTTTTTTGTACTCGTTGAAATGGAAAGACTAAAACAAATGGTTTAATTGAAGCATTAGATGATGCAGAATTTGAAGGGAATTTACTATTTCTTTTAAATTCAAGTATAAACTTTATTAAAAGAAATAGTAAAAACATGTGAAGAAAAGGAGTTTTATATCGTATTCAATATCCTGAATATCCTATAAAAGCAGTGCAAGAAGTAATAGTTAATGCTTTGATTCATCGAGATTATTCAATTATTGGTAGTGAAGTACATATTGACATGTATGATGATAGATTAGAAGTATATTCTCCTGGTGGTATGTTTGATGGGAATTTTGTCCAAAATATCGACCTTTATAATGTATGATCAATTCGCCGTAATCCTATTTTAGCTGATTTATTTGCACGAATGAATTTAATGGAAAGAAGAGGTTCTGGTTTAAAAAATATTTTAGAAATTTATGCATCTAAAGAAAATTATCGAGAAGATTTAAAACCGGAGTTTCGTTCAACTGAATCTGCTTTCTTTGTTGTTTTAAAAAATTTAAATTATCAAGAGCAAAATATATCAAAAAATGAAAATCAAAAAATATCCCCTAGCCAAAGACGTAAAAAAATCATAGAATTTATCGGACAAAATCCAAAAACAACAGCTTATGAATTAACAAAAATATTTTTTGTTTCAATTAATACCATTGAACGCGATCTAGCTAAATTAGTACATGATGGATATGTTGAATTTGTGGGATCATCAAAAAATGGTGAATGAAAAATTAAAACTAATCAATAAATCACGTTGTTAAAAAATTTTCTCTTCTTTAATCTTGTTATAATTCTTAGTTAGTAATAGTTTAGGTGGTTAAACGTGAAAAAATATGATGTAATTGTCATTGGTGCTGGACACGCAGGATTAGAAGCAGCTTTTGCAACTTCTAATCTTAATTTACAAACAGCATTAATTACATTGGATGAAAAAGGGATTGGGATGATGCCATGCAATCCTTCAATAGGAGGGCCGGCTAAAGGAATTGTTACACGTGAAATTGATGCCCTTGGTGGTATTCAAGGTAAGGCAGCTGATGCTACAACAATGCAAATGAAAATCTTAAATTCATCAAAAGGACCAGGTGTTTGAGCGATTCGTGCACAAATTGATAAAATTGCTTACCAACGATGATTTAAGCAACAAATTAAACAACAAAAAAATTTAGATTTAATCATTGCTGAGGTTAGTGATCTATTAGTAGAAAATAACATTGTTAAAGGTGTTGTTTTAAGTGATCAAAAAATCATCCAAGCTGATTATGTAATTATTACAACAGGAACTTATTTAAAATCAATTACTCATCGTGGTTCGGTTTGTGTTGATGAAGGAGCAGATGGTACAAAAAATGCTAAATTTTTAAGTGATGCATTAGTGAAATTAGGATTTGAGTTAATTCGTTTAAAAACAGGGACTCCTGCAAGAATTAAAAAAGATTCGATTGATTTTACAAATATGATTCTTGAACCTGGTACAAATCAAAAAATTGCTTTTAGTCACTATCACCCTGTTTATAAACCTTATGACGAACAACTTCCTTGTCATATTATTTATACAAACGAACAAACTCACCAAATTATTCGAGAAAATTTAAATAAATCAGCAATGTATGGGGGTATGATTTCTGGAATTGGGCCACGTTATTGCCCTTCAATTGAAGATAAAATTGTTAAATTTAGTGAAAAACCGCGTCACCAAATTTTTGTTGAACCAGAATCTTATGAATTAGATTCAATGTACTTAGGTGGTTTTTCTACTTCAATGCCAATCGATGTTCAAGAAAAAATGATTCGTAGTTTACCAGGATTAGAAAACTGTGAAATACTAAAATATGCTTACGCTATTGAATATGATGCTATTGATCCAACCCAACTATATCCTAGTTTAGAATCAAAATTAGTTAATAATTTATTTTTTGCTGGTCAAATCAATGGAACTAGTGGCTATGAAGAAGCAGCGGCCCAAGGATTAATGGCTGCAATTAATGTTAATCAAAAATATCAAAATAAAGAACCTGTCATTTTAGGTCGTGATCAAGCATATATTGGCGTTATGATTGATGATATTGTTACCAAAGGCGTTGTTGAACCATATCGTTTACTAACAAGCCGTGCGGAACACCGATTAGCATTAAGAAATGATAATGCTGATGATCGATTAATGAAAATTGGTTTTGAAATTGGTTTATTAAAACCAGAAGTATATGACCAATACTTAAATAATTTAAAACAAATTAAAGAAATCTTAAACTGATTAAAAACAACAACTGTTGGCCAAATTGATGACTTAAAATTTACAACTCTAAAAACAAATAGTTATTTAATCGATTATTTAAAACGTCCTGAAATTAAATTAAATGATTTATTAATTTATTGTCCTATAAAAATTGAAGATGAACAAATTATCAATAAGGTACAAATTCAAGTTAAATTTGAAGGTTATATTAAAAATCAAGAAGAAAACTTAAAACAATTAAAGCGTTTAAATAATATTAAACTTCATGCAATTGTTGATTATAAAGAAGTGCCAAATATTTCTTTAGAAACAATTGATAAATTAAATAAGATTAAACCTCTTGATCTTGAACAAGCAAGTCGGATTAGTGGGGTTAATTTAACAGACATTGCAATGATTAAATATTATTTAGAAAGAATTAAAAATGACTAGAAAAGATTTTTTTAATGTTTTAACTAGATATTTTCCTTGAGTTGATGAACAAACTTTTATGAGTTTTGAAAAGTATAAAACAATTATTCAAAAATATAATCAAATTTTTAATTTGACAAGACTTGATAGTGATGATAAGATTTACCAAAATTTCTTTTTAGATTCACTTGCACCTTATAAAGAACTAGATTTTTTTACACAAAATACTAATCTTAAATTAATTGATATTGGTTCTGGTTCTGGAATTCCAGGGGTTGTTTTAAAGATTATTTTTAAAAATTTAAATTTAACATTATTAGAAGCTAATCAAAAACGATGTGAATTTCTTAAAATATTAACTCAAGAATTAGGTTTAAATGATGTCTTAATTTGAAATATGCGTGCTGAAGATTTAACACAATCGATGCGTGAATCTTTTGATATTGCAACTTCAAGAGCTGTTGCATCATTAGATAAAATTTTAGAAATTTCAGCCGCATTTGTTAAAGTTAATGGTTATTTAATTCAACCAAAATCAATAAAATTTTATGAAGAAGAACTAAAAGCAAAAAATATTATAAAAACTTTGTATTTAGAAAGAATCGCTTTAAAAGATTTTTGAGAAAATGATTATCATCATTTAGTTGGTGTTTATTTAAAAAAACAAATCACTCCACTACAATTTCCTCGTCCTTGAAATCTAATTCTTAAAAAACCACTCTAATTATTTATAAAGGAGTTATATTATGAGTTCATTACCAGTTATTATTTTAGTAATTGGCATTTTTGGGTCTATTTTTCTAGTAATAGGATATATTCCCCAAGTAATTAAAGTTATCAAAACAAAACGAACAGATGGAATTTCATTAACATTTTTAATTTCTTTAAACATCGCTTGTTTTCTATTTGTAATATATAGTATATTAGTAATGATTTTTAACAAACATAATGGTATTCCTACTGCTTTACCATTATGTCTTGCAAACACCATAGTAGGAATTTTAGGTCTAGTTATTTTAATTTATAAAGTTAAAAACATTAAAAAAGCTAAATTATATTTAATGGATGAAAAGACATATTATGAAAAATATGTCTTAAATAATTTATAATGTCTCCAATTAGTATTGAATTAATAATTCAAATAAGTATTGGATTAAGTGCTAGCCTAATTTTATTGTTTGCTTTCTTACCACAAACACTTTTAACTATTAAAACTAAAAATACTGCTGCATTAACAATTAGTATGTTTATTATTTGTTTTATTGCACGTTTATGTTTTAGTTTAAGTGCTATTCTAACAATTATCGTCTATATTCATAATCAGAATTATGGTTTATCTTTATATGCGCTTACACTTCCTGTTTTAATATGTCATGGTATTAATATGTTATTAAATTTAATTATTGCTTTTATAAAAATCAATAATGTTTATAAAGCCAAAATCCATAAAATGAATGAGAATGAATATATTATTTTTGCTTATGCTCAAAAATTAAAGGAAAAAGTATCGATAAAAAATAAATAATTTACACAATAAATTTACATAAATATTTATTTTATTTTTTTAAAAAATTGGTCCTTTTTTTTTTTTTTTTTGCTTTATAATTAAAAAACATTCAAGTTGACTATAAATTTGAGTATTTACACTTCATATTCAATGATGCAAAAGCATTATTAGTTAGGAAAAATTTATGAAAAAGTTAAAATTAATCACTTTAAGTTTATCGCCTATTCCTATCGTCGCGATCATGGCTACAGCATGTAGTACAACAAAAAAAGAAAAAGAAGTAGATCATAAACAAGATGAATTAACTACTATAAATAATAATTTACAATTAGAATATCCACAAAACAAAGATATAGAAACATCAGCAGCAATAAAGGAAGAAATAAAGCCTTTAAATTTGCCGTCTGGTGTTGTTTTTAGCATTAAAGAAATTAGTCCTAAAGCAAATGATGAAAGTACATTAATAGTTAAATACACCCTTAAAAAGGGAAACATAACTAAAGAATTTAAAAAAGAAATTAATGGATTTAAAAAAATTCGTAAAGATCAAAATATCGCTAATCTAAAAGACATGCATGATGATTTTAAAACAATTTTTAATAACATTAAATTAAAAGATACGTTCGATTTTAAACTAAAAGGTCTTTTTAACTCAAATTTTGGTGAATACGATAAATTATTACCATCACAAATTATTAACAATTTTGCACAAGGTGTAGAAACTAGAATTAATACTAACCAAGATCGTTTAGGTATTGAAGTTATAGATGTTAGTTATCCAAATAAAAATTTTGGTTCTGCTAATCGTGAAGGTAATTTAAAAATGTCACTATTAGTAACTGATAAAAAAACAAAACAAGTTTTCATTAAATCAATAATTGCGTATGGTTTTAAAACTAATGCAATGGGTTTAGATGAGAATGGCTCAATTCCAGGTGGTGGTGCTGATCTTGTTAAACCTAAAGTTGATGAAAATAATTATTTTAATAAAACTCAATTAGAAAGATATGAAATTGATAATCAAGCTTATTTAAATGGTTTAAAAGGCCAACATATGGGTAAAACTTGACAACAAGTGCGTCCTGAACTTGGTGATAATGAACAAAAAATTAATGAATTTGATCAAAAAGCTAAAGGTGTAAGTCAAGATGCTTATGCGTCTGCTGCTTATAAAGGTTTTACACTCCCAGTGTATGATAAAAATGGTGATTTTAAAGGTTTATCAATTAATGAAAGTAGTTACGGCCAAGCTCGTTCATGAGTTGATACAAGAGGTCGTGACGAATGAAAAACCACTGGTTTACCACGAACTTTACCAAACGAAAAATATCGTGATGAAGCTTTACAAACATTAGGGATTAGTTCTTTAACTTTAAAAGATGGAAAAAAAGATACTTGAGATAAATCTTCAGGAACAACATGAATCTTAGATTATCAAAAAACAAATGATAATAAATATCCTACAAAATGATATTTTGCAACTAATTTACATGTTGCAGATACTATTACAGATAAAACAACTTCTATTGATTTAATGAAATTAATGGATAGTGTTAAAACTAAAACAACTTTACGTTTAAGTAATTTAGATGAAAATATTTATCGTTTTGGTTTTGCAAGTAAAAATAACAAATTTTTATTAAATCATGGTCTTAAAAAAATATATGATGGTCGAAATTTCTTAAAAACTAAACCTACAGAATACTTAACAGAACAACAAAAAGAAAAATACAAAGATGCAGGTAGTTTTGTTGATTTTGCAGTTTTTGAATTAGATTTCGAACAATTAAAATTACTTAATGTTTGAAACAATCAACTTAACTCAAATGGCGGTGTAATTACTAAATATGATCATTCTTCTGCACAAGATCTTGCAAAAATTATTACAAGTGATTATGCAAACCATAGTGATAAACAAATTAAATTCTTATCCAAATCATATTTAAATGATTATTCTAAAATTGATGTTCCTCTAAGATCTACATCATATAAATTCGAAGGAAAAGATGAATTATTTGCTCTTGGATGACCTAATTCAACTAGAGATGGTTTTTTTGAACGTTATGTGGATGATGATCAAACTAAATATCGTACAACAGATAATTTTAGTTTATGAACAAATTCTGATTATCGTTTTTTTGGCAAACTAACAGAGCAAGAAGGTGGGCAACCCGCTTTCCCAACAGAACGAACTGAACGTGGTAATTACTTGTCTTATGCGATTGGTTACCGTAGTTTTATCGATAAACCTGGTGTTCTAGATGCCTTTATTTCTGCTCCTCATACTGGCAATGATTTATATAAATCTCTTGATGGTAAAAAATATATTAATATGGGTTTGGAATATATGCCTAGACATTATGCTCCAGCAGGAGGTGCATCTGGTTCAAGTGTGCGAAATCAAAACAATGAAATTGTAGGAATTTATCATGTAAGTAATGAATTTGCTAGCACCGGCTTAGCAACTGCTTTTCGTTCTGAAGGATATGATTATAAAGGCTTATATGGTGATAAATACAAAGAATTACCACAATATGACTTAATTTATGGCGGTGGAAAAGATCAAGCTACAGGTCAATCATATCGTGAAAAAATGAAAGAAATATATAAAAATAATAACAATATTAAAACAGCATTATTTCCAAATGGTTTTGACCAATATGATGAAAAGTTTAAATTTAATAACAATCAAAGTAAATAAAGGTTAGGTTTAAATAATAAAAATGAAATTTATTAAACGTAAAACAAAATTATTAACGATTACAATTGGTGCAGTTGCTGTTAGTTCAATCTTATTAGGAGGAATTTTTTATGGTACAAGTCAAAAAAGTCCCTCAAGTTTTGGTATTGCTTCTATTGATCAAAAAGAAAATTTTATTAATAAAGACAATTTAGATTACCAAAAAGCAAGACCATCAATTAAAGATAGTAACTTAAAAGAAATTCCCAAACCTAAACCTCAACCAAAACCTAAGCCTCAACCAACTCCATTTCCAGATCCCATCCCAACACCTCCAAAAAAAGAAGAGCTAAAAAAACCAGATATTAAACCAGAAGAACCTAAAAAACCTGAAATTAAACCAGAACCAAAACCTGAGCCTATTCCTCAACCAGCCCCTCCTATTGAAACAAAACCAAAAGAAGAATTATTACCACCCAACCCTCCGCCTCCAAAAGAAGAACCAAAACCAGAACCAAATCCGCAACCACAGCCACAACAGGTTCCAAACAATAGTAATTCACGAATTATAGAAATTAATGGTGTAAGAGTGGAAGCTGAAGTTGAAGTCCCTCCCCCTCGTGATATTGCTGAATATGATAAACAAAATAATCTGGTTAACCCTAATCCATACATTAATGATAGTGTTGGTAAAATTAAAAACGTAAAGGTTACCGATGAATTGCGAAAAGCAACAGGTAAATTGGTCCAAGGAAATTTAGGACGTTGAGATTACAAACATTTAATTAATGATTTATTAACTCTTAAACCAGAGGAAATAGAAAAATATGTAAAAAACGATAAAAGCGGTTATTATGCAAAAGTTTGATATCGTTTTTCAAGATTATTTGAATCTGAAAATGTTGTTAATTTTTTAACTGAACAAGGTAAAAAAGAATATCCTGAAATGAAGTCTAAATTTGTTTCAAAAGACCATAAATATGCATGGCTTTATCAACATTTAGACTTAACAAAGTTTACACAATTATCAAATGAATCAGAATCTTATTTAAAAGAAGGCTATACACCTGATCCTGATAATGCATATGTTGATGAAAATGGAAAAATTAGTTCTCACGCATATTCACCTGCCAAAGGTTATAATTCGGTAACAAGTCGAATGGAAAATGATAATTGAAATCGGCGTGTTTTCGGTTATAAAAGTTGGTATGGACGTACTCCTGGAAATTTAGTTGAAGGTAATTATCCTGGTTGAAAGAAAACAAATGTAACCCAAGAATTCCATCAATATGGCGTTAGCGATGGTGATGGAATTACAGTTAATAAATTAACTCGTGAAAAAACTGAAGATGGTCGTTTAAATGAAGGATATGTTATTGATATTGATGCTGATAATCCACAAGGTTATGAAAAAACAAAAAAATTAATTCAAACTTTAAAAGAAAAAAATATCAATATCACAGGGTATAGAATTCATAATATGGGGAAATCAGATTCAAGTCAAAAATTTGTTGATATTCTAAAAACATTACCAAATCAATTGCCTTTATTAGAATTGTTTTTCTCCGCTGGTTCGCATAATACCTCTTCATTAATCGCACTGAAGGATAAAAAAATTAAAGAATTAGGTTTGTTCACACTAGGAAATTCATTATTAGATGAATGATCAATTAATCCTAATGCCTTACGAAATGTTGAATGAATTAATTCAAACGATTATAATGTTTCATTTAATTATAAACAAGGTGCAGATATCGCTACTCGTATTACTTTTGATACTTTAGCTTTTGATGAAAGTGATTATAATGACAATGCATCTGACATTAAATCAAAATTAAAACAAATTAATGATGGTTTACGAATGGTTTATTGAACAAGAAATAATGAACCAATTTTTCAGGGTAGTTTTGGACCTGGTTTAGATCCTGACCACAAAGAATCAGGCAACAGTTATCCACAAGGATTAGATTTTAGTCGTGTCCCTCAAATTCGCTCATTACGGGGTTTAATATTTAAAGATGAACAAAAAACATCAAATAATAAAGATCGTAAATTACGAAGAATTAATTTTTACAATAACAGCACAACTTATAAAATGTCTATTGAGGACTTAAATGAAGCCGGATTTAACGAACATATTGTAAGTGGTGAACCTGGTGAAAAAAGTAAAATTACTTTTAGCAATGGTTCTGGTACAACTAAAATCCAAATTGATGGTGATCAAGAATTGTCAGCAAATGGAATTAGTAACTTATCAGCAATGTTTAATTTTGCTGAAAGTTTACAACGGACAATTGTTGTAAATAATACCAACTCACCACTTGCAAATCAACTAAGAAATGCGGGATATAGTGTTGAATCAACAACTAACGCTGGACTAATAGATATTTAATCTTTATTTAAAAATTTTGGACAAAAATGTATGAAAAAAAATAAGTTAACTACTCTCGCTTTAATATTACCAATTACAATTTTAACTCCTATTGTTATTGCCTCATGTACAAATAAAACTAAAGTTAAAAAATCATCATCTTTAGATAAGATTGCTTCAAATTTAAAATTAGAGTATTTTAATAACAAAGCTAATACTAAAGCATCAAGTGTGCAAAAAGATGAAATTAAAAAACCACTAAATTTACCAAATGATGTTGTTTTTAGTGTTAAAGACGTTTTTGTTAGTCACAAAGATCAAAGTGTATTAATTGTCAAATACACTTTAAAAAAAGGGAATGAAATTCAAGAGTATACTTATGAAATTAAAGGTTTTAAATCTGTTTATGAAAAAGATAAAATCGTTAATGATTTAAGTCAAGCAAACGAAGATTTTAAAAAAATTGTTAATAATATCAGGTTAAAAGATACATTTGATTTTAAACTAGCAGCTTTTCCTAATCAAAATTATGACCAATTATTACCATCACAAATTTATAAAAATTATTATCAAGGTATTGAAATTCAACAACACAAATATCAAAATGAATTAGATATTAAAATTATTAATTTTTTATATCCTGATGGTGATTTTGGTTCGGCAAATAAAAATGGTACTTTAAAATTATCATTAATGTTAACTGATAAAAAAAATAATCAAGTTTACTATAAACTTTTAGAGGTTTCTGGCTTTAAATCTAATCCCTATGGTGTTGATGAAAATGGAACAATTCCTGGAATTGGTACAGAACGTTTAAAACCTAAAAATCAAGACGATTATTTTTCAAAAACTCAATTGCAACGTTATGAAATTGATAATGAAGGATATCTTCAAATTTTAAAACGCCAAAATAATGATAAAAATTGAAAAGAGTTGCGCCCTGATTTAAATGCAACTGTTAGTGATATTAAACATTTTGATGAAAAAGCAAAAAACGTGGGTCAAGATTCTTATGAATCTGCTGCTTATAAAGGTTTTACATTACCTGTTTATGAAAGTGATGGCAAAATCAGTGGTTTAGCATTAGCAGGTAAAGATACACCAAAAGGGCCGTCATGAGTTGACGCAATTGGACGTAACCAATGACAAATTGGTGGTTTACCACGAACATTGCCAAATGAAAAATATCGACAAGAAGCAATGCAAACATTTAGTTTAGGAATTTTAAATAACGATTCGCACAAGAACAATACTTATAATAAAACTGCAGGTACAACATGAATTTTAGATTATCAAAAAACAAGTGATAATAAATATCCTACAAAATGATATTTTGCTACAAATTTACATGTTGCAGATGCGATAAATGAAAATACTTTATCGATTAATTTAATGCGATTAATGGATTCTGCACAAATTAAAACTACTTTTAGATTAAGTAATTTAGATGAGAATATTTATAATTTTGGTTTTCGAAGTAAAGAACATGGCAAAAATCTTTTAAACCATGGACTTAAAAAAATTTTTGATGGCCGTGATTTTTTAAAAACAAAACCGGCTGAATATTTGATTAATTCACAAAAAGAAAAATATAAAGATGTTGGTAATTTTACAGATTTTGCTGTTTTTGAATTAGATTTTGAAAAATTAGAATTAGTTAATGTTTGAAAAAATTTTTTAGGTGAAAATAATGGGTTAGTAACAAAATATAATAATTACAATCCTCAAGAGTTGGCTAAAGTCATTACAAGTAATTATGCTAATAATAAAAATAACCAGATTAAATTCTTATCTAAATCATATTTAAGTGATTATTCTAAAATTGATGTTCCTTTAAAATATAGACAAGAAGACGCTAAAACATGATTTAAAAAATACGATGAATTATTTGCATTAGGTTGACCTAATTCAACAGAAGATTTCTTCTTTAAAGCTTATGTTGATGATGACCAATTAAAATACCGTACTCGCGATAATTTTAGTTTATGAACAAATTCTGATTACCGATTTTTTAATAATTTAACTCAACAAGAAGGTGGTCAGCCTGCTTTTCCGCCAGAACGTACTGAACGAGGTAATTATTTATCTTATGCAATTGGATTCCGTAGTTTTATTCAAAAACCTGGAATAGTTGACGCATTTATTGCAGTTCCCCAAATTGGGAATAATTTATACACATCCAGTGATAATAAAAAATATATTAATATGGGACTAGAGTATTTACCCAAACATTTTGCACCAGCAGGTGGTGCTTCTGGAACAAGTGTACGCAATCAAAAAAATGAATTGGTTGCTATCTATCATGCTAAATATGATTCATCAAAAACAGGTCTAGCTGCTGCTTTTCGATCTGAAGGTTATGATTATCAAGGATTATATGGAAATTATAATTTACCACAATATGATTTAATTTATGGCGGTGGAAAAGATCAAACCGAAAAGAAATCATACCGAGAAGCGATGAAGGATATATATCAAAATAACAATATTAAAACAGCATTATTTCCTGATGGATTCGATAAGATTCCTGATGAATTCAAATTTAATAATAATTAAAAATAAATTATCTTTAATTGAAAGGATTGTTGGTATATGAAATTTATTAAACGTAAAACAAAATTATTAACGATTACAATTGGTGCAGTTGCTGTGAGTTCAATTTTATTAGGAGGAATTTTTTATGGTACAAGTCAAAAAAGTCCTTCAAGTTTTGGTATTGCTTCTATTGATCAAAAAGAAAATTTTATTAATAAAGACAATTTAGATTACCAAAAAGCAAGACCATCAATTAAAGATAATAACTTAAAAGAAATTCCCAAACCAAAACCTCAGCCAAAACCAGAACCACAACCAACACCATTTCCAGATCCCATCCCAACACCTCCAAAAAAAGAAGAGTTAAAAAAACCAGAGATTAAACCAGAAGAGCCTAAAAAGCCTGAAATTAAACCAGAACCAATTCCTAAACCAAAACCTCAGCCTATTCCTCAACCAACACCTCCTGTTGAAACTAAACCAAAAGAAGAATTATTACCACCAAGCCCACCACCTCCAAAAGAAGAACCAAAACCAGAACCAGATCCACAACCACAACCACAACAAATTCCTAACCAAAGTACTGTTAGAAAAATTGAATTGAATGGTGTTTTAGTGGACGCTGAAGTTGAAGTTCCACCCCCTCGTCAAACTTTTAAATATGATCAGGATAATGGATTATCAAATCTTAATCCCTATACAAATATAAGTGTTGGGAAAATTAAAAAAGTTTTTGTAACAGATGAGTTGCGGCACAAATCAGCAGATTTAGTGCGCGGGAATTTAAAGCGTGGCGATTATCAAAGTTTAGTTAAAGATTTATTAGATCCAAATATAAAACCAGAGGAAATTGATAGTTATATCGCAATGGTTGATAAAAGTGGATATCACGCTAAATTATGAAGTAAATTTAAAAAACTATTTGATACAGACAATGTTGTTAATTTTCTGAATGAACAAGGTAAAAAAGAATATCCTAATATGAAAACAAAATTTGTTTCTGATGCGCACAAGTATGCTTGATTATATGCTCATTTAGATTTTTCAAAATTCACTAAACTTTCAGCCAATTCTGAAAAGTATTTACAAGAAGGTTTAACGCCTGATCCAGATAATTCATATGTTAATGAAAACGGCGAATTAGACTCATATGCTTATTCACCTGCTAAAGAATATAATACAGTAACAAGCCGTTTAGCAAATGATAATGCTAACCGAAGGGTGTTTGGGTATAACGAATGATACAATCGTAGTCCAAATGGTTTAGCTAATGGTGATTATCCTGGTTGAAATAAATCAGATGCAACAGCTGAGTTCAAACAGTATGGCATTAAAGATGGTGATGGTATTAAAGTTTATAAACTAGAGCGCCAAAAACCACAAGAAGGTAAATTAAATACTGGATATATTGTTGATATTGATGCTGATAATCCTGATGGATACCAAAAAACAAAAGAATTAATTCAAAAATTAAATAATCAAAACAAAAAAATTACAGGATATCGAATTCGTAACATGGGGAAATCAGATTCAGGACAAAAGTTCTCTGATATTCTAAAAGCCTTACCAAATGAATTGCCATTGCTTGAATTGTTCTTTTCTGCTGGTTCACATAATACGTCAGCACTTTCTGCTCTTGAAACTAAACATATTAAAGAACTTGGGTTGTATACATTAGGTAATTCATTATTAGATGAATGATCAATTAATCCAAATGCGCTTCGAAAAGTAGAATGAATTAATTCTAATGATTATAATGTTTCGTCAGAATATAAACAAGGATCAGATATTGCTACACGAATTACTTTTGATACTCTTTCTTTTGATAAAAATGATTTCAATGATAATGCAAATGATCTTCGAACAAAATTAAAACGTATTAATGATGGATTACGAATGGTTTATTGAACAAGAAATAATGAACCATTCTTTCAAGGTGGATTTGGTCCAGGATTAGATCCTGATCATAAAGAGGTGGGAAATAGTTATCCCCAAGGTTTAGACTTTAGTCGTGTCCCTCAAATTCGCTCATTACGGGGTTTAATATTTAAAGACGAACAAAAAGCTTCAAACAATCGTGAACGTAAATTAAGAAGAGTTAATTTCTTTAATGATAAAGAAAATTATGAAATGTCAATTAATGATTTAAATGAAGCTGGTTTTAGTGAACATATTGTGACTAATGAACCAATGCCTCCTAAAAGTAAAATTACTTTTAGTAATGGTAATGCTACAAAACGTATTTACATTAAAGGCAATGGTAGTCTAACTGCAAGTGGTATTCAAAATTTAGCTACATTATTTAATCTAGCAGAAAGTTTAGATTCTAAATCTGTTGTTGTTGATTCTAATAATTCAGAATTAAAGTCACAATTAGAAGGTTTAGGATATAAAGTAAGTGATGCTTCTGACGCTAACTATATTGATATTTAATAGAAGGAATTATTATGAAAATTAAAAAACTTATTGCAAGCGTTAGTATAATCACACCAATTGTGTTTGTTTCAGCTTTAGCAGCGTCTTGTGTTAATAATAAACATGAAGATAAAAATATTGTGCATAATAGTAGTGACCATGGTAATAATAAAAAAAATAATTTTACTAATGATTTAAATCAAAATTTAACAACCCACGAATTTAAATCAAATTTAATAAGTACTTCCCAATTAGATAATATAGCCAAATTAATTAATTTTACTTATGAAAATAAAGCTAATACTTATTTAAAAAATATACTTATTAATCAATTAAAACATTCACCTATTCAAAATCAAGATTTTAAACTAGAAATTATAGGTTTATATCCTAAACAAAATTCCTTGCAGGATTTAATTATTTATTATAAGTTAACTAACCAAAAAACAAAGGAGATTAAAGGTTATTATTTTGAATTAAATGGTTTTAAAAAACCAGATAACACCATTTTTTCAAATCAATTAACGCCTGAGCTAAAAAAAATTATTGATACTATTCAATTCAAAAAAACTTTTGATCTCGTAATTAATAATCAACAAGTATTAAATTATGAAAATGTTTTACCTTCACAAATTAAAAATCAATTATTAATGGGTTTAAAAGTTATTCAAAAAGAGTATACAGACAAAATTAAACTAAGTGTACTTGATGTTTTATTCATTAACGAGGGTGGGCAAATTAACGCTAATAAGTTGGGGGCTTTTTCTTTATTATTAGAGGTTTTAGATCTTAAAAGTAAAAAAACTTACCAAATTCTTATTCCTGTTGATAAATTTAAAACTAATCCTTATGGTGCTGATGAATATGATTTATTGCCAACGCAAGTTAATAACGGATTTGCACCAACTAGCTTAGCTCAAATTAATCAATACAATAATGCTGATCAACAAACACGTTATTTATATGATAATGAAAATTATTTAAAATCATTAAAAGCATATCAAAGAAATGTAAATTGATACCAAATACGCCAAGATTTAATTAATAATAAACAAAAAATTAATGAATTTGATCAAAAAGCACCACAAGTTTTTCAAGATAGTTATGAATCTGCTGCTCGTAAAGGTTTTACTTTACCAGTATATGATGAAGATGGTAAATACCAAGGTTTATCATTTAACGAAACTGAAATTGGAAAAAGTGTTTCGTGAGTTGATGCAATTGGAAAAGATCAATGGAAAATAAATGGTTTGGCTAGAACTTTGCCTAATGATATGTATAAGCAAATTGCTTTACAAACATTTGGCATTCAAATTCAAACGCCAAATGGTAAACCACGCGAATCTGATGTAGTAGCTGGAACAATGTGAATTATGGATTATCAAAAAAGAAATGATAATAAATATCCTACTAAATGGTATTTTGGCACAAATTTACATGTTGCAGAAGCATTAAAGTCAACAACAACAGTTTTTGGTATTAATAAGATAATGCCAACAGTTAAGACTAAAACTACTTTGGGTTTAGCGAACGCTGATGATAATATTTATCGTTTTAGTTTAGTTAGTAAAGATAATCAACCAAATATGGATAAACCTATTAGTAATGGTATTAAAACAATCTATGATGGTCGTGACTTCTTAAAATTAAATCCTAGTGACTTATTAACATCAAAATTAAAAAATAAATACCATAACCTACAAGAATTTGTTGATTTTGCTGTTTTTGAAATTGATTTTGAAAAAATAAAACTCGGCTCTATAACGAAAAATTTTTATAGTGGATCAGATTGAAGTGTTGATAAATATAATAATTTAGATCCAAGTGAATTAGCTAAATTAATCACAAACGATTATGCACAAAAACCAAATGAACAAATAAAATTTTTATCAAAATCTTATTTAAATGATTATGCACGTATTAATGTACCTTTGGATTCAAAATACAATCAGGCTTTTGACACAAATAAATATGATGAATTATATGCTGTAGGTTGGCCAAGCTCTGCTTCTGATTATTTCTTAGACCCAATTAAAGATCAGAAACAATTTGAATCAAGACGTGAAAGTTATAGTTTATGAATTAATTCTAATTATCAATTTTATAATAAATTAAATCATAATCCTCCTTTGTTTCCTCTTACACAAATAAATCGTGGTGATTTCTTGTCATATAATATTGGTTATCGTAGTTTTACTAATAAACCTGGATTGCTTGATGCTTTTATTGCTAGTCCTATTACTGGTAAAACTGTACATAAATCAACTGATAATCAAAATTATATTGGGTTTGGTTTAAATTATTTACCGATGCATTATTCTCCAATTGGGGGTTCATCAGGGACAAGTCTACGTAATCAAAAAAATGAATTAGTGGGAATTTGGCATGTTGGTAATGGTTTTGCCCAAACAGGTTTGGCTGCTGCTTTTCGATCTGAAGGCTATGATTATCATGGATTATATGGTAGTTATAACTTACCACAATATGATTTGATTTATGGTGGTGGAAAAGATCAAAAAACTTCCTATCGCCAAGCAATGATGGCGTTATATAAAAATAAAAATATTGCAACCGCTTTATTCCCTAATGGTTTTGGTGAAGATCAAATCCCCAGTGCTTTTAAATTTAAAAATTAATTAACTTTTATATTGAATTAGAAAATTATATGATGAATACAAAAACATTTACTTCCGTTAATAGAGTAATTTATGATGATAACTATTCGCTAAAGCAACAACAAAAAAGTAGCTTTATCAATCAATTTTTAAAAGGATTGTTATCTGCTATTACTTTATTATTCTTTATTTTACTTTTAATCTTTGCTGAAAATACGTTGTTTGGTTTAGGTTTTGGTGATGAGAATAAATCAATGATGATTTCAAAATCGCTAAATGCTTTTTTTGATTTACACAGCCCTAAATATTTACAACTTAACTTTTTAATTGTATTTCGCTTTTTTATTTTAAGTTTTACACTGTTTTATACCTTAATCAAAAATTTCACAAATCTTTATTGGCATCGAGCAACCATTAAAAAATACTTACCATGATTCGTTTTATATTTAGTGATTGCAACAATTAGTTTCTTACTATTTTTTACTTTTTTTAGTGTTTGACCAAAAGAAGTTTTTAATTTAGTATTTTTACTATTAGTGTTATTTTTATTAAATTTAAGTTATGAAATATTTAATTATTTTATTTCTAAAAAAACTAACCCTTTATTATACGATAATTATAAAAATTTAATTATTGCCATGGTTTTTCAAGCGTTATTACTATTATTTGTAATAATTACCCCCCTTGTATGAATTAATACAGGTAAAAGTCCTAACTTTTTATTTGTTGATAATCGTTTCTACACACGCATAGTTGATATCTTCACTGTTCAATCAGGTAAAAATTTTATTATTTTAATTGCTTTCTTCTTCTTTTTAATTACATTTATTGTTTTAGCAAATACTAATTTTTTCGCTTTAGTAATTAATAAACGTTACGATCGTAATTATGTTAAGAATAATTTATGATTTATTTTACTTTTATTTAGTGCTATATTTATTTGATTATTAAGAGTTTTTGCATATAAGCACGAAAATGAAAATCTACCGGTTGGTAACAACCATTTATTATGAGTTTATATTTTACAAAGTTTTTTTGCAATAATCATATTAATACTATATATGGTATTCACTTTAAAAAAACGCTTAAGCGTAAAGAGTAGTTTAAATACTTTATTAAATTTAGTAGTCACTCAAACAATTCTAAGTTTAAGCTTGTTTTTAGTAACATTATTTAATTCTAAGAGTGTGGTTTCTTTAATTAATGTTTTTATTACAATTACTGTACAAATGAGTGTGTTTGGAATCTACATATTTCAAAATAAAAACATTTCAACTAAATTATTGGTGCTATTAAAGGTTATTATGATTTTAATTATTTTAACCGCAGCAATTGTAGGTTTTGATTATTTATTAACATCTGATCATCATAATAACTATTTATTCTCAAACATTCAACCGAAAATGAATTTAGTACAAATCATGTTGTTATTAAATTTTAGTTTGAATTTTACTTTAATTAGTTATTTAACAATTAAATTTGCAATGGTAATTTTTAAAATCAATAAGCTAAATAAGGAGTTAAACAATGAAAAAAAATAAATCTAATCTGACACCTACAACAAATTATTTTGATGTATTTAATACTTATAAAGAGAAAAAAGCAAGTGTTGATTTAATTACATATGAAGAATTAATGGCATCAGTTTTATTTGATAATAAATTAGGTTTTGAATCAGAGGTTTATTTAGATTTTGTTAAAAAATTTACATTAGCTTTTGAAAAAAAACTAGATATTTGATTTGAAAATTTTATTATTAATTTCAATTTAAATTTAAAATTTTCAACAACTATTATGATTCCAATATTAGTAACTAAAGCTAATTCTACAACTGATGCAATTAATTTTAGAAATGATCAAAATCCTGTTTATAATAATTTTTTAATTTCATATAACCAAAAAATTAAAAAATTATTACTTCAAAATCATCCTGTTCAAATTCTTCCTCATTTGATTTTATTTAAATCAAATCTTAATGGTAGTTTAGTGCTCGTATTTAGTGAAAAAATTATCGCTTCAATTGAACAAAAATCAGGAAATTAAGTATGCAATTAAAAGATTTAATTAATAAAAAAAAGAATTTAAATAATATTAATTTAAAAGTGAGTAACGAACGTAATATTTTTTTAATTAATATTATGAAACTAAATCAGCGATTAACTTTTTTTAGTAAAAATGCTTTTGAAATAAAAGAATCAATTCTAAGTTTAAAGCGTATTTATAATATTAAACATGATATGTTAAGACATGAGGAACGAAAGATTTTTAAGTTTTTAAATAAAATTAATGATCGTGTTTTATGGATTTATTTAACTGAAGAGCAAAAATATAGTACTGATTCATATTCGCGTTATGAACAAAAAATCTTAAAAACGATTAAATCAAATCGTGATGATTTTATTTTGATCGGTCAAGGAGCAATTGAATTTGGTAAGAATCATAATTTAAATGTTCTGCAAACATTTAATGACTCAAATATTAAAAATTTAACTACACAATTAACTAAAATGATTATGATTTTATATACTTTTGATAATTATAAAAAAGTTAATTTTGTTATTAATTCTAATAAAAACTATGATGGACACTTTACTATATTACCAATGAATGAATTTAGTTTTGATAAATTTATTAATTTACAAAAATGCGATTCTAATATTATTGATTTTCAAAAAGTAAAAATTTATCCAAATTTAAATGAATTTATTAATGTCCAAATTAATGTTTTCTTAGTTAATATTATTAATACTTTAATTACTGAATCATCTTTTTATAAAACAAAAAATGGACTAGTAGCTACTAATAATATCCTAAAAGAACTAGATGATAACCTTTCAAAAATTCAACGTAAAATTACACGTGTAAAGACAGAGTTGCAAATTGAAGAAATAAATTTATTAGCAAGACAAAATATGAATGAAGATGATAATGATAACGATGGTGGTGTTTATGAATCATAATTATAATATTCAAATTACATTACTTGAAAACAAAAAGTTTAATATTGATAATGGTTTATTATATGTAAATGTTAATGAAGAAAATACATGACAAAAAATTGAAAACAACACCGTTTTAGCCTATGAAATAATCTTATTAAAAATAGTTGATGATGATTATAAAAAAACTTTTTATTTATTTCTAAAAAACACACATATTTCTGTTTTAAATAACATCGTTAAAATTCAAGCATTAAATGATTTACATTTCTTTATTAAAGATGGATTAAACAAAAAAAATAATCATAAAAAAGAGTTGGTAGATAAATATAAAAATATTACTAATAATATTTTAGAACTTGAAGCTAAACAACAATTAGGATTAACTCTAAGTGAATTTTTAGATCTTGATAATCTAAAACAAGAACAATATATAACTAATATGCAAATTAGATTAAATTTAGTGGAGTATAAAAAAGATGAGAAATAAATTGAAAATAAAATTAAAATTTTTAGCTTCACTAGCAACAATACCAATTGTAGCTTCACCATTAATTTTTTCTGTTGCAGCTAATGAGGATGGTAAACAAGAAAACAATAATGGTAATAATCATGATCCTAATCAACAAAAAAAACCAAAAATTCCTAAAAACGATCCTAATTTTAATACTTTTAAAACTGATGCTGATAAAACTGTTAAAGATAGTTTAGAAAAAGGAATTAATGCTGCTATTGTTTATGTAAAATCACGTCAAGAAGAAATTTTAGAAAATAAAGAAATTGAGTTTAAGAAAAAAATTCAACAACTAATTTATTTAAAAAATCTGCAGTCATATCTTGAGAAAAATAAAGAAAACATCTTAAAAAATCCTAATGATTATGGTTTTTATTTAAATACTCCTCAAATTTTAGGAACTCTTAAAAATTATGATATTAAAGATATTGAGTTTAATGGAGAAACATATAAGCAAATCAAAGTTGGCAAAACTGATCCTTTAAATTATCAAAAAGCCGTTGCTCCAAAAGGAAAAATTAGTGATGTACAAAGTGATCAAATTAATGATGTTGAAGAAACAAAATACAAAGATACGTTAAAAAAATATGAATCTGAATTTTTAAAAGAAATTAATAAATTAATTTATGATGAAAATGATGTTCCACAAATTAACAAAGATGTTGAGTTAACACGTGATGAAAAAGGACAATTTAATACAACTTTACCTAAAGGTTATAATGATTGAAACGCTTATTTTATTTCTAAAATAAAAGATCGTGTTACAGCATTTGATTTAAAACAAAATCAACAAACAAACGAAGATAAACAAGAAGAACAGCCAAATGAGCAAAAAGATCCCGATACACCACCACCATTACCACCACCATTAGTAGAAGGCGATCATAATGAAATAGATCTTCCCCCAACACAAGCTAATGCTATTATTTCTTCTTTACCACTATTATTACCTTATATTAGTCCTATATATTCAAATGAATCATTAAGTGGATTAAAATCAAAATTTGATAGTTTAAAACCAGAGCTTAAACAAACATTATTTTATTTTAATAACCCAATTAACACACGTTATCTTTATAGTGTATTAGATTTTGGCGTTAATGGTAGTAGTATGATTAATATTAAAGTTAAAATTATTGATCAAGTTAATCCTAAACTACAACGTACATATATTATTAATAAATATGATCCAATTTTAGATATTAATTTTAATAGTCTAAAATTAAATGAAGTTAATGCTATTAAACAAATTTTTGTTAACTTGTATAAACATTTAGGACTAGATGAGAAAATTGATTATAAGAAATTACGTAACTTTTATATTAGAAATGCTTTATTTACAATGATTGAAGCTGCTCAAAAACTTATTTTGCGATTCAATCAAATTGATAAAGATAATAAAATTATTGGTCAAAAAACTTTTGCAACATTACAAAATGAATATTTAGAAAAATACAAACAAAAACTTGTAAACAACACCGATGATAAGGAAAGATTATTAAATGAGTTTAATAATTTAACAAAAGAAAATTTCTTTCGTTATTTAAATAACACTTTAATCAATAATGATTATTATTGATATCAACTAGTTGGTGCTTATAAACAAGTTAGCTTACAGTTTAGTGAAGTTCTTCGATTAAATAAAGATAAAATTAAAGCTAATATTGCAAGTATTAAAGGTGATGAAAACACCATTGCAAATTTATATAAATTAAATAACCAATTAATTTATCAATTATCGGCGATTGTTGCACAACGTAGTTTTAATTCTCAACAATGATATCAGTCATATTTAAATGTTTTACAACCGATTAAAGAAAATTTTGATTTAATGTCTATATTAACAAATCAAACCGATATTAAAACAAATAAGGACAAAGCTAAGGATTTTAAAAATGCATATGATAGTGCTTTAAAATCTCTTGAAAGACAAAAACAAGTAAATAAACAAATTCGTCGTAAGATTGGAATTGCGTTTATAGTAATTAGCTTACTGGTTTTAATAATTAATTTAATTATTTATGGATTGATTAAAAAACTAAAAAATAAAAAAGTTATTCTAATTATTAATAGTGTGATAATGGTGTTGACAATCATTGTTTTAATAATGGGAATTATTCTAATAATTTAGAAAGGAAATAGAAAAATGCAAAACACTAAGGCAAACCCTCGTATTAAATCGATTTTTAACTATATTGTAACTGTTAGTGGAATCTATGATTATCAACATCATCAAATATTTGTTTTAAAAAATGATCCAACAGTAAAATTATTTGTTATTTCAGCAAAAGAAGATCTTGCTTATTTATTAATTAGCAATGAACATGCTAATATTAATATCGGCGATGAAATCATTGAAACAAATCGTGAAGAAAATGTTGTTACTGATTTAAATTATTTCGGGAAGATTATAGATATTAATAATAATTTTATTTGACCAAAACCACTTAATAATCTTCCATCAGCAAAAATTGCTCAAACTAATCCTGCATTTGGTTTAGCTCATAATTTAATGACAGTGAAAACTTTAAATGAGCAATTAAATACTGGAATTGTAGCAATTGATTTATTAATTCCGATTGGCAAAGGACAAAGGGAATTAATTATTGGTGATCGTCAAACTGGAAAAACGCATATTGCTTTAAATACTATTATTAATCAAAGCCGTGCTGGCACAAAATGTATTTATGTAGCAATTGGTCAAAAACGCGAATCATTAACTACTGTTTATGAAACTTTAAAGGCTCATGACGCATTAAAAAACACAATTATTATCGATGCTCCTTTAACAGCATATGAACAATATTTAGCACCATATATTGGAATGGCTCATGCTGAAAACTTATCGTATCAAGATGATGTATTAATTGTATTTGATGATCTAACAAAACACGCTAATATTATTCGTGAAATGGCTTTATTAACAAATAAACCAGTTGGTAAAGAAGCAATGCCTTCAGATGTATTCTTCTCACATTCATCATTACTTGAACGTGCTGGTTCATTCAAAAATCGTAAAACAATTACTGCTTTGCCGATTTTACAAACAGTTGATGGGGATATTACTTCATTAATCTCTTCAAATATTATTTCTATTACTGATGGTCAAATTGTAACTTCAACTGATTTATTTGCTGCTGGTAAAGTTCCTGCTATTAATATTGATTTATCAGTTTCAAGAACAGGTTCAAGTGTACAATCAAGAATGATTACTAAAGTAGCAGGTGAAATTAATAAAATTTTTAGACAATACAAACGTCATTTAAAATTAGCAATGTTAGATTATGAATTTAATAAAGAAACTAGCCTTTTATTGCATAAAGGTAAATTAATAGATAAAATGTTTTTACAAAAAGGTTTCTCTTTATTTTCATACCGTTTTATTGTTTTAAGTACTAAGCTAATTGCCTGAGGCATTTTAAAAGGTGTTAAAGATGAACAAAAAGCATTTATGTTGTTAGATTATGCGATTAATAATTATGAAGATGCTCAAAAAGCATTCAATACTATTTCAACAACTCAAAATTATGATGATAAAATTATGAAAAACTACTTTGCTTTCATCCTAAAACAATACTCTGATTACTTAAATTTAAATTGAGAAGTTGAATTAGAACATAGTTTTATTCCTTTACAAAATGAGTTTTTAATGAATGTAGCAAAATTTTTAGGAGATAAATAATGAATAACGGCCGAGTTATAAAAATCTGATCAGATATTGTCGAAATTGAATTTAAAAACAGCTTACCAGCTTTAAATCACTTATTAACAACTCATGATGGCAATACTTTCTTATTAGTTAAAAGATTAGTTGATGCTACACATGCGAGAGCAATTGTTGTTTATGCATCAAAAGATTTAGCAATTAATGATATTGTTATTAATACAAACAAAAGTTTTATGGTCCCAGTTGGTAATGATTCAAAAAATAATATTTATAATTTTTGGGGACATCCTTTATTAGAAACTAATAAAACACCTAAATATGTTGAAATGAATTCAACAATTTTAAACGAGCGATATGTTGATAAAAGTATAGAAATTGTAGAGACAGGAATTAAAGCAATTGATTTTTTTATGCCAATTCTAAAAGGATATAAATTAGGAATCTTTGGTGGTGCTGGAGTTGGTAAAACAGTTTTAATGAAAGAAATCATTTTTAACTTAAACAGACATAAACAAGCAAATTCAAATATTTTTATTGGTTCAGGAGAGCGTTCACGTGAAGCAATTGAATTATATAATGAATTGACTGCTTCTAATTTAATGCCTAATTCAGTAATGTTTATTTCTAAGATGAATGAAGCTCCTGGTGCACGTACTTCAATTGTACCAATTGGAATTACTGCTGCTGAATATTTACGTGATCAAAACAAAGAAAATGTTTTATTGTTTATTGATAATATTTATCGTTTTATTCAAGCTGAAAATGAAGTTTCTACTGCATTAGGTAAAAAACCGTCTGTTGGAGGTTATCAATCAACACTAGAAAGTGATGTTACACATATTCAAAATCGATTATTTAGAAATAAAAATGGATCAATTACCTCATTTCAAACAATTTTTTTGCCAATGGATGATTTAAGTGATCCATCAGCTGTCGCTGTATTTAATCATTTAGATGGAAAATTAGTATTATCGCGAGCACAAGCTGCTAAAAATATTTTTCCAGCATTTGATCCATTAGCAAGCTCAACAAACGCTATTGATCCTAAAATTATTGGTGAACGTCATTATAATGCAATTATCGAAACTAAAAAAGTTTTAAAAGCTTATAAAGATCTAGAAGATGTTATTTTAATTTTAGGTTTTGATGAACTAGATGCTGAAAGTAAAATTATTGTTAAAAAAGCTTTACAGCTAGAAATGTTCTTTACACAAAACTTTTTTATGACTGAACATTTCACAAAAGCTCCTGGTCAGTTTGTTCCATTAAAAGAGACTGTTGAAAGTGTAATTCGTATTTTAGAAGGTAAATATTTAAAACAAAGTCCAGATGTTTTTGCCTATATTGGTTCAAATAAGGATATTTCTCAAGATCATTAATAATTATTAATTTATATTAAATTTAAACCTTGGTAACTATATTAATTACCGAGGTTTTTTATTAAAATGTTCACCATTAATATGTTTTGTTTAGTTTCAAAAGTGCAAAATAAAACATCTTTTTTTAAAAAAGTTTTCAAAAGCTAATTTTAGATATTTAAAAATATTATAAATATTTGTATAAATTTTTCATCATTTTTTCTTATAGCTTGTTAAGATTTTTATATATAACTTGTCTATACAAATTAAGGAGTTTAAAATAAAGTGGTTTGTAAAAAAAATAAATTGAAAATGTTTGGTTTTATTGCTATTGGAACTGTTTTAACTAGTGCAATAATTCCTTTTTTAGTTACATGCGCAAAAGAGAAAAAAATAAAGCAAGAAGTTATAAAACCAAAACAAATAAGTAATTTAAATCTTGAAGCTAAAAATATTGATATTGTTTTAAAAAATAATGTCTTCAATAAAGAAACAGCAAAATTTACATTGGATTTTGACATAAATAAAGAGTTAAATTTACAAATGGTTGCTGTTTTTGATGTTTTTGATAAAAAAAATAACTGATTAAAAACAATTAAAACAGCTAAAACAAATTTAGTGGATAAAAAATTTGTTTTTGACAGTTTAGATGAGAATGTTAAATATCTACTAAAACAAATTGATTTTTACGATCCAAAAGCGCCTAATCAAATTATTTATACAAAAAACAATCAGACATTTAGTTTTGATACTAAAATGGATGATAGCATCGCTAGAACTGTTGAACTAATTATTATTAGTGGGTTTAATGGTTTTGTTGATGAACAAATTGTTCCAGATTATAATAAACAAACTACATATTTAAAAGCACCCGGGTTAATTCGATTATTAAATGAAGTTAATAAAATTAAAAAAGAACGAGGTGCGAATAATGTTGCTGTAGTTATGGGTGGAAATAATTATTATGAAAATAATATATCTTCATTAACTAGAGCTCAATATACAACAGCCGCATTAAAGTTTTTAAATCCAGTAGCTACTAGCATCGCTAATACTGATTTAAATTGGGGTTATGATTTAATTGACGAAAAAACAAAGCATTTTAAAGAATACGTCAAAGCTTTATATGGTAATGATGGTATTTTATCTGTTAACTCTTATCTAAAAGATGGTGATAAAAACATACAAATGACTAATAATTCAAAAATTATTAACTTTGGTGGTTTTAAATTAGGATTAATTGGAGCAATGAGTAATTTAATTAATTTTGAATCTAATCTTAATTTAACAAAAAATATTCAATGATTTAGTAATGAACAAACAGCTAATTTGATTAATGATGAGGCCGATAAGCTTAAGGATCAAGGTGTTAAAAATATTGTTTTATTATCAAGTTTTTTAGCAGATAAAGCAAAGGAAAATGACACTTTAACTAATGATGAAAAATCTTTTTATAAAGAAGCATCACGAATGAGTAAATTAATAGATAATAATGTCAATTTAATGAGTGTTATTAATCCTTATAAAGCACTTAATGTAATTGCTAATAATAAAAATAATCAACCTTTGAATGTTGTTCAATCAAAACCTAATAGTGGTTTTGTACGATATATGATCACACTAAATCCTTATAACCGTATTTTAAAAACAGAAATTAAGCTTGTTGATAAACTGTCAACAAGCGGTGATAATGCAGGATATCATTATATAACTCAACAAGATTTATTAAATCCACAAAAAAATCCTGATTTCAATCAAAATTTGATTACATTAAATAATTTTCTTAATAATTATAAAGATCATGAATACGAGCAATTAAGAGAAAAAATACTGATTAAAAATGATAAAAAAGTCAATTTATATTCTTCAACATATTCACTTAGTTCCTTAGGAGCGTTTATAAGTGATATATTAGCAAAATACTATCAAGCTCCTTTTTATAATAAACAAACACATACTTTTGAATTTAAACAAACACAAGCTATCCTATTAGCTCCTCATGCTATTCGTTCTTTGGTTTCTGCTAAACCTATTAAACTAGAAAATTTAAATCAAATTTTACCTTTTAATGATACGTTAGTATCAGGTGAAATTAGTGTTAAAAATCTTAAAGAATTTTTAATTAACAAGAATAAATCTCAAAATTACAACGAACCATATCAATTTAGTAATACGCTAAAAATTGAATATGAAAACTTACAAGAAACAAAAACAAAGAAAAAATCAAGAAAAAAACAATCAGAACAAAGTTATAGTGATAATGAAAAAAAAGATGGTCTAATTTTAAGTCATCAAAAAAGAGTTATTAAAGTCTTTTTGAAAAATTCAGATGGTACGTATACAGAATTAAATGATAATCAAAAAATTATAGTAAGTGTTATGAATTCGATCTTAAAATTTAATAAAAAATTTAGTGAGGCTCTTTTAAATAAAACATACGCTGACCAAACTAATATTCGTGATTTGCTAAGAACATACTTACCAAAAGAAACACAAATCGATCAAAAATATGATCAAAGTGTGTTTAGTAAAAATGATGCTCGTTTTTATAAAAAAAAAGAAACAGACCAAAATAAATCACTTATTTACAATCATATTAATGAAAGTATTAATAATAAACAAGCCGATCATTTAAGAATTGGGCACTGAAATGTTCTACATCAGACTGGATTAAATGATCCTAAGAACTATGCTTTAGCTAAAACAATTTTATTTAATAAATACGATATTATTGGATTAACAGAAATTTGGCCTGCAGAAAATAACAATCAGGAACAAAACAATTTACCGTTGATAGGAATCATTAAGTATTTAAATCAGTTAAGTGGCTCAGATGATTATGATTTTTTAATTAGTGATAATCTTAAAGGTAGTGAAAATGATAAATTAGCAACAAACGAACATACAAGTACTGAACGAATTGGGATAATTTATAATAAGAAAAAAGTTAAACCTATTCCGTTTGCAAATAATAAAATCGGTTATATTTATTCAAATCCATTACAACCTGGCAAATATGAAAAAGCAGAAACTGATTATTCACGTCCACCATTTGCTATTAAATTTGCTTCAATTGGTCAAATTAAAAATGATTTTACATTGGTTTTCGGTCATCTTGATAGTCCTGGAACTTATCCATCAAATACAGAATATCAAAAGAAAGAGCATCTACTAAAACGTACAGAATTAGTTAATAAAAAAGTAATTTCAACGAACCAAATGATTAATAAATATGAACAAGGAAGTCGTGAAGTGGACGATGCTGAACGATTAGTCAAAGTTATGGAAGAAATTAAAAAAATTGATAACAATAAGGATGATGATTATTTCTTTTTAGGTGATACTAATATTCGTTTACATAATCAACAATGAGTTTTTAGAACTTTAATTCAAAATGGTTATAGTAGTGTTTTTAGCGATAATGACTTATTTAAATCATCATTAAGTGTAAATCCTGGTGAATTTTCAAACCCTTATGATAAAATTTTTTCTCAAGTTAATGGTTTAACTATTTCTAATCCAAGTATTTATAATTTATGAAATGTATATGAAGATCAAATTGTTGATCAAGAATGGTTAAAAAAAGTGCGTAGTTTATACCCTAATGAATATCATGATAAATTATTTTATATTCGTAACGATATTTCTGATCATGCACCTACATTCTTTGATTTAGGTTTAAATAAAAACGACACTAAATAAGCAAAATGATAACCTCGTGTTCAAAAATTAAACTAGGGGTTATCATTTTGTTAAATGTTATAATAATAGTTATTAATTATCAAGGAATAAAAATATGGCATTAACAATTGTTTTAATCTTGTTTAGTGTTCTAGCTTTAATAATAGGTTTATTATTATCAAGAACTTCACCATCTGGTGGATTGTCAAGTTTAAATGGACAAGATCTAGAAATTTTTAAAAAAACAAAAGACCGTGGTTGAATTAAAGGTTTACAAGTCTTTATGTTTTTATTAACAATTGTTATGATTTTAATAATAATTTTTTATAGAGTGAGCTAGATGAGTGATTTTACGAAACAAACTATTACCGAAGTAATTTCTAAGGAAGAGCGCCCAATACCCGCTGCTATTCTTGCTAAAAAAGTGTTAGAAAAAATACCCACTTTAAATAAAACTGATGTTTATAAACTAATTGATTTATTAATTCAAGAAAATACTATTAAAAAATTAGAAAATAATCGATTGGTTATTGGTTATCTAGATTATGAATTTGATCACGAGATCAAACAAGGAATAATTACTATCAATTCAAAAGGTGATGGCTTCATTAAAGAAGATAATACTGAAATTGAATATTACGTCAACAAAAAATATTTAAATGGGGCTTTAAAAAAAGATTCAGTTAAATTTGTAAAGTTAAAAAAAGAACCTAAAAACAACCTGCAAGATGCTGCTGTAATTGAAATTGTTGGGCATGCTAAAGACCATTATGTTGGTCAATTTATAACTCTTCCTAATGGTGGTTATTACATTTTTGTTGACGATCCTTTATTTTACTTAAATATAAATTTAAAAGATACTACCGGTTTAGTTAATGGTCACAAAATTCTTTTTAAAATTATTTCGCAAACAACAAAAGATGCTATTGCAGAATTAGTTCATATCATAGGTCATAAAAACGATGTTGGTTCTGATGTTTTATCAATCGTTTATGATAATGGTATTGATCCAACTTTTGATCCACAAGTTGTTGATTTGGCTTCAAAATTAGAATTTTATGTTGATGAACATCAAAACAAAATTCGACGTTCAATAATTGATCGTGAAATTATTTCTATTGATCCTGTTGGTTCTAAAGATATTGATGATGCTGTTTATGTTAAAAAATTAAATGATCAACGTTACTTTTTAGGTATTTCTATTGCTGATGTTAGTTTTTATGTACAACCTAACACCATTCTTGATGCTGATGCTTTTAAAAGGGGAACATCAACATATTTAGTTGATCGTGTTATTCCAATGCTTCCGCATAATATTTCTAATAATATTTGTTCATTAAACGAAGGTGAGTTTCGCATGTGTATAACATGTGATATGGTAATTGACAAAGATGGAAAAATATGTTGAAAAGATGTTTATCCTGCTATCATGAAAAATTATCGTCAAATGTCATATGATGAAGTTAATGATTTTTATGAAGGAAAATCACGTTTTGAATCAGCAACATTAACTATGAAAGAAATGTTATTAGAAGCTAAAGAATTGCACCATATTTTAAGAAATAAAAAAATTAAAGATGGTTATGTTGATTTTGATATTAAAGAACCAAAAATTATTTTAGATGAAACTGGTGTCCCTATTGATATTAAAATTTACGAACGTAAAACTGCTCAAATGATGGTTGAAGATTTTATGATTGCAGCTAATGAGGCTGTAACTATGTTTGCTGAAGAACACATGGATAAAACACTAAAAGAATTTAATTTAGAAATGCCTTTTATCTATCGTGTTCATGATAAACCATCAATTATTAACTTACAAAAATTTGAAATTGAGGCAAAAAAATTGAGTTTCAATATCTCACATGATTTTGAGAACATTCAACCTAACACTATTTCAAATTGACTAAAAATGAATGATAATCATGTTAACTTACCATTAATTAGTAAATTATTATTAAGATCAATGGCTAAAGCTTCTTATGAAATTATTAATACGGGACATTTTGGTTTAGCATCTGATAACTATACACACTTTACATCACCAATACGGCGTTATCCAGATTTAATTGTACACCGTTTATTATGAATGTTTATTTTTGATTCTCAAAGTTATACAGATAAACAACGAGTAGAATTAGTAAATAAACTGAAGTTAATTACTGAAGAATCAAATAAAAATGAAATTATAGCAGTTAAGACTGAACGTGATGTTAATGCTGCTAAATTTGCTGAATATATGAATTTACATATTGGTAAAGAATTTATTGGTGTGGTAACAACAGTTTCTAGTTTTGGTGTTTTTGTTGAACTCGAAAATACGATTGAAGGTTTAATTAGAATTAAAAATTTAAAAGATGATTTTTATGATTTTATTCCTGAAAACATGACTTTAGTTGGTCAAAAAAGAAAGAAAATTATTACTGTTGGCAATAAAGTTCGTGTACGTGTTATTGAAGCTAACAAACTTACAAGAAAAATTGATTTTGAACTAGTTGCACAATAAAAAATATAAGACTTAAAAAAATTTATTTATAATACACAAAGAAGATGACTAAAGTAAATATTTAGTCGTTTTTTAATTGCTAATTATCATAAAATGTTACATTTTTATATAAAATATAACTTTTTTGCGATTAAAATTTCTTTTGAACTAATAAACTTTACAATTTATTAATAAAGAAAGGTTTTTAGTATTTTTAATAATGATTGTATCAAATAAACACGCACGTCGAAATTATGAATTACTAGATTTTTTTGAATGTGGAATCGTTTTAAAAGGAACTGAAGTTAAATCTATTTCACGTGCTAATTGCTCAATTAATGAAGCTTATGTCCAAATAATTAAAAATCAAGCTTTAATTTTAAATATGCATGTTGCAAATTTTTTTGAGGGTAATAACTTTAATCAAGATCCATACCGGAATCGAAAACTATTATTACACAAAAAAGAAATTATTAGACTTCAACATTTAGTGAAAACACAACATATGACAATTGTGCCAACCAAAATTTATTGGAAAAATAATAAACTAAAGATAGAGATTGCTTTAGGAAAAGGTAAACAGCTTCATGATAAACGTGAGGATATAAAAAAAAGAGATTTAGCGCGTGAAACGCGTTTATTTTAATATTAAAGGAGGAATTATAATGAAAAGTGTTTGAAAGATTTTGTTAATAAAACATAAATTTTTATTTTTCTTTATTTTATTTTTAAATTTAGTACAAGTTTTATTAGTAATTGGTTCTCCTCTTTTATTATCAATTCTTAATAATGGTAATTATGCATCAACAACCGCTGACGCTTTTATTTATGGCGGTGTGTTAATCGCCATGGCTATAGGATGAGCAATATTTTCTATTTTTACAAATTACATTTCAACCGTTTTTCAAATTATTGTTGAAAGTCGTTTACAAAAGAAAATTTTTGAAAAAACAATGTTCTTAACATATGCAGATAAACAAAAATTTAATCAATCAATTTTATTAACAACTTCAATAAAAGACGTCGAAGAACTTTCAAAAACAATTATCTTTTTAACACGACCTGTAATTATGGGAATTTTTTATTTCATTGGAGCAATTATATTATTATTCATTATTAGTGGTAATTTATGACAAATCCCTTTAACTGTAGTTTTGATGGCTTTATCAACAATTATTATTTTTATGATTATTTCAAAATTTAATGCTAAAAATTGAAATAAAATGCGTGTTGCTAATGATGAATTAGCAAAAGTGTCATTAGAAAATGTTAGTGGAGTAAAAGTTATTCGTTCTTTCTTATTAGATGCATTTTTTTTAACAAAATTAGATTTTAAATGAAAAGATAATGTTTCTAAATCTATTATTGCCAATCGAATTAGTAAAGTAAGCATAGCTATTGTTACTTTTATCATTTATGTTATTACTCCGGTTATTTTAATTATTGGAATTTGTACTAATTCTATGAAAGCAACAAACATTTTATCAATCATGCAATCAACTAATTTATTAGTTTTAGGAATTGCGTTAATTGGTTTTGGAAGCGAACAGTATTTTAGATCTATAGTAAGTATTAAAAGAATTAATGATTTAATTTTTTATAAAAATCAAATTGTTTATAATGGTACAAAAGAAATTAATAACAATAATATTGAATTCAAAAATGTAAGTTTTAGGTATCATAATTCAAAAAAATGAGCAGTAAAAAATATTTCTTTTTCAATTAAAGACACTGATCGCGTAGGAATCATTGGAACCACAGGTGCTGGAAAAACGACATTAATTAAATTACTGATGCATCAATTAGAACCAAATAGCGGAGAAATATTTATTGATAATATTCCTTTAAAAGATATTAAAAAAACAAGTTTGCAAGCTGCTTTTAGCCTTAATGAACAAGTCCCTTTGCTTTTTTATGGAACTATCCGTGATAACGTATTATTTGGTTTAGAGGATATTGATGAAAACAAAATCAACGAAGTTATTGAAATTGCACAAGCTAAGGATTTTATTGAATCTAAGAATGAAAAATTAGAATCTCTAATAGCACAAAGGGCTCAAAATCTATCTGGAGGACAAAAACAACGTCTAGCAATGGCACGTGCATTGTTGCGCGACGCAAGAACTTTAGTGCTAGATGATTCAACTAGCGCATTAGACATGATCACTGAAAAAAAGTTTTTAACTGCACTAAAACAACAAATTAAATTTAAGAATTCGATTATTATTGCTCAAAGAATTAATATTATTTTAGACTGTGACAAAATTATTGTAATGGATAAAGGTAAGATTGTTGGAATTGGTAATAATGAGACATTACTTAAAACTTGTGCAGTTTATAAAAATATCTATCAACATCAAATAAATAAAGGAGCAGAATATGAGCCAACAACTACAAACCAATAAACCTGTAAGTAAACTTTATTTAATTTATTATTATTTTAAGGATTTTAAAAAAGTATTTATTGGTTTGTCAATTGCCATTATTTTTTGATTATCAACAACTATTGCAGCAACTTTTTTATTACAAGAAACGGTTGATAAATATGCTATTGCTAATGGTATTGTTGATACAGTAGTTAAAATGTGTGCAGGATTGATAGGAATTTATTTTTTCTCATTTATATTTTTACTAATTATCAACGAATTTGCCATTCGAATATCTTTTAAGATTGAATCCCATTTATCAATGATGGTGATTAATCGTATTCGTTATTTACCAATGAAGTATTTTGATATTAATAAAAGCGGAGAAATTTTTACTAAAACACTAAGTGATCCAACAAGTGTACAAGATGGAATTGTTAATTTTTATATTGAGTTAAATAAAGCTATATTTGGCGCAATTGGTTTTGGAACTGCTTTATTAATAACAAGTCCTTATATTGCTTTAATTGGAATTGTCATTTATGTTCTAGTAATGCTATTTAATATTTTGATATTTAAAAAATCCCATCAATTAATGCGCATAAAACGGCAAAATTTTGGTGAAATGAACGGATATATTGAAGAAATGATTTATGGGCAAAACGTTGTAGCTAATTTTAATGAACAAGTTTTTTTCATAAAAAAATTAGATAATATGATTAAAAAATTATATAAAAATTGAATTAAAGCTCAATATAGTTCTCAAATTATTTTTCCTTGAAGTATCTTTTCTATGCGATTAATGAATGCCGTTTTAATTGTGTCTTATTTATTAATTTCTATTAAAGGAATTCATTTGCCAGGGCTTATAAATAATATTGATCCAACAACGAATATTATTTCTTTTGGTGGTTTAGTTAGTATTTCATTATTTGGTAATTTCTTTTGTGATAATTTTAGTCAATTATCAAATACTATTCCTATTTTTATCGTTGCTAGAACATCACTTGCTAAAATTGATGAAATTGTTAAAACTCCTAATGAGATAAATTGAAATGAAAAATTAATAATTGATGCCTCCCAAGGCATTGAAGTGCGTTTTGAAAATGTTAATTTTAATTATTTAAAAAATACACCTACCTTAAAAAATATTAATTTTGTTGCCAAAAGGAACCAAAGAATTGCTATTATTGGACCCACAGGTGCTGGAAAAACAACAATTACTAACTTAATTAATAAATTTTATGATATTAATAATGGCTTTATTTATTTTAACGATGTTGACATTACAAATAAATCCCGTGAAAGTGTACGTGAACATATTTCTATTGTTTTACAAGATCCTTTTTTATTTAGTGAAAGTATTTATGAGAATATCAAAAAAGGTAAAATGAATGCAACAAAAGAAGAAATTATTGCAGCAGCTAAAAAAGCTCAAGTTCATGATTTGATCCTTTCATTTCCAAAAGATTATGCTACAGTTATTGATGAAAAACAAAACTTATCACAAGGTCAAAAACAATTAATAACTATCGCTCGTGCTATTATTTCTGATGCGAAAATTATTATTTTAGACGAAGCAACAAGCTCTGTGGATACGCAAACCGAACATAAATTACAATTAGCAATTAATAATTTACTAAAAGGACGAACATCATTTATTATTGCCCATCGATTATCAACAATTATTAACTCTGATTTAATTCTTGTAATTAAAGATGGTGAAATTATTGCACAAGGGAATCATGATTATTTGATTAAGAATTCGCCTTTTTATCAAGAACTTTACTATGCAAATTTTAATGAATAATTGCAATAGTATGATATTATAAAGGTAGTTAAATACATATATAACAATCAGGAGATTCCATGACATCGTTAGACATAACCACGTTAGTAATTAGTATACTTTCGTTAATTGCAACACTATCAATTTCTTTTAATATTTATTTTATTGAATTAAGAAAACAACGCGAACGTAAAATTGAACGTCTACAACAAGAAGCAAAACAATTTATTATTAATAACTTAGATGAAAAAGATTTTATTGTTTTGTGTCAATTTATTTATAAATTATATAAACATGATAAACATACAAGAAAGATTCATTATGAATTTGTTTTATTAAATGAACCTGTTCAAAAAGAGGTTTTTAAACAACTAGAAATTCTAAATATAGTTGATTTTAAAGATAATGAATGAATTGCTAATAAATTTAGTATATTAAAAGAGATTGTTAACGATTACCAATTAGGTAATTGAGATGATTATAAATTTTATGAAAATTTTAATTTCGCTTATACCTTATTTAGAGAAGAAAAATGCGATGCGGTCTTTGAAGAAATAAAGCAAAATAAATTCGGTGGTAAAAACTTATCATTTCATGAGTATTTAAATGAATACGCGCATCGTAGCAAAGAAAGCGATATGCTTGCTCCAATCGATTATTTTATTGATCAAAATAATTTAAATAATGTTTTTGATCGTCAAAAACACGCTATTTATAAACTTTATTTGCTTGATTTGATTTTAAATGAATTGTGCCGTTCAATGAATAATGACCATCGTATTAATAATGAATTTAAGTATTTTGATTGCGAAGTTATTTATGTTGAAGATTTATATTTAAAAATTTTATATAAACTTTATTTTCAATTAAATTAATTATTATAAATACTATAAAAAATTAATAATAAAACACTTTATATTTATTTAAAATTTTGAATATGAGATAATAAAAAGATAGTGTATTTTAATAATAGGTAAATTATTTATGGAAAGAAAATTTAGTGATCAAGAATTAATCCGTCGTACACACTTACAAGAATTAAAAAACCAAAATAAAAATCCTTTTTTAATAACAAAAGTTGATAGAAGTATGTTTTTAAAAGACTTTGCTGAAAAATATAAAAATTTTAGCAAAGAAGAATTACATAATATGGATTTAGAGAAATTGACACTTGCTGGTCGTTTAATCGGAATACGTCAAACCTTTGGGATTATTCAAGATTTTTCAGCAAAGTTACAAATTTATATTAATAAAAAAAATGTTGCTCCAGAGGTTTTTTCAACTTTTAAATCATTAGATATTGGTGATATTATTGAATTAGAAGGTGTAGCAATGAAAACTAATAGTGATGAAATTACTTTAAATGTTACAAACATTCGATTAGTTGCTAAAAGTCTAAAAGTTCTCCCTGAAAAGTATCATGGATTGGTTGATGAAGAAATTAAAGCACGTCAACGTTATTTAGATTTAATTGTTAACGATGAGACAAAAAATACTTTTATAAAACGATCTTTAATTATACGTGAAATGCGTAATTGGTTAGATAATAAAGGTTTTTTTGAAGTTGAAACGCCTGTTTTGCAAGATATTTTAAGTGGTGCTGCTGCTCGTCCTTTTATTACACATCATAACACTTTAAATAAAGAATATTATTTAAGAATTGCTACTGAAATTGCTTTAAAAAAGTGTATTATTGGGGGTTTTGAAAAAGTATATGAAATAGGTAGAATTTTTCGAAATGAGGGAATGGATAGTACTCATAATCCTGAATTTACTTCAGTTGAATTATATATTGCATATGTTGATTTATGATATATTATGCAACTAACTGAAGATTTAATTCGTCATATTGCTACTAAATTAAACTTATTAAATCCTACATTTAGAGGTTTTAGTATTGATTTAAATAAACCTTTTAAAAAAGCTCATATGGTTGATTTAATTAATGAATATGTTGGTGTTAATTTTTTTGAAGTTAAAAGCGATGAACAAGCAATTGAATTAGCAAAAAAACACCATGTTAAATTATTAGATCATCAAAAAAACTTTGGTCATATCGTTAATGCTTTTTTTGAAACTTTTGTTGAAGAAAAATTAGTTGAACCAACATTTGTATATGGTCATCCGTTACAAGTTTCTCCACTTACTAAAAAGAATCAGAGTGATCCTCGTTTTGTAGATCGTTTTGAATTATTTATTTGTCAAAAAGAATTTGCTAATGCTTATAGTGAAATTAATGATCCAATTGATCAATATGAACGTTTTATCGCACAGTTAGAAGAAGCTAAATTAGGAAATGACGAAGCAAGTGAATTAGATATGGAATTTATTGAGGCCTTAGAATATGGTATGCCTCCAACAGGTGGTTTGGGAATCGGTGTTGATCGTTTAGTAATGCTGTTAACAAGTAATGATAGTATTCGTAATGTTTTATTATTTCCTCACATGAAAGATAAAACAAAATAAAATTGAAATGGAATTAGTAATATTTTAAAGGTGATATATAAAAACTAATAACAATACTACAAAATGTTAATAAATCAAACATTTTGTATTTTTTTTTATTTCCAATTGTTAAACCTAAAGTGTTAAAGTTTATACTAATTAAAAAATATGATAAATTAAAACTATCATAAAAAAATACAACTATCAAATACAATGTTATAATTCATCACAATCAGATAGTAAAAAAAAGTAGGTGTGTGAGTTTAAAAACCACTAGTGCCTATATATATATAATTGATTGATTTGAATTATTTTTTTAATTCTATTAATAAATGATTAAGTAAATCTGTAAAATTAGTAAAATATTGGCAATGTGATAATATCATACCATCTAAAAACTTGTTTAAAACTTCATTGCGGTGTTTGTGAACTCTTCAATCAGTATTTAAAATATAAATTTTTAACGTTGGTTTAACGTGTTTTTGAAGTGCTACAAAATAACCCATTTCAGCCATGGTGCCATCATCAGTGTTATCAATATCTATAATTGCGATATCAGTTTGATCAATAAATTTAATATCTGATTCATAAAAAAAGATATTTGGTTTATGTGCATTAGCACCTAATTCGTCGTTAACTTCGATCGGATTAAAAAGATCAAGTTCATAATTTGGTAATTCATCTTTAAATGTTTTTCTAATTAAACTAGCTTGTTGTTTTCGATCATTAATTTCAGCTAAGGTGAATAAAGGACCAGCTAAATAAATTTTGATTTTTTTACTCATGAATTTACTCCTAAAAATTTAATGTGTTAATCATAACATTATTTAAATTTGCTTTTATTAGTTTTTTTATTTTTGGAATCCAAAAGTTAAAAATAAAAAAAGATTGACTGTGTTAACTACTCAATCTTTTTTTATGAAAAACGAAAAATAGATTTTAATTAATTTGATTACAATAATGAAATTTCTATTTTATTAATTTCGTGGTTTAATTCTTTGGTTTTTAATTCCAATGATTTTCTTTTTTGATTTAAACTAATAATTCTTGATAAATTTATTATTAAATAAAATAGCCCTACTATGGTTAAAAAAAAGATTAAAATAACGGCTATGATTATTTTCATCATAAATTCATTTTTAATTTTATCTAATTCAAACTCAAGATCATTAACTTTTTGCTTCAATTCTATTAATTTAGTTTTTTGTTCGTCACTCATATTAAACCTTTTGAAATTTAAATATTATTTTTTGCTTCTTTTTTTATTTAGTAAAGCAATTGTTTGTTTATAATCTTTAATTCATAATTTAACAATTTCTAAGGCTTCTTGTCATGCTTGTGGTTTTGTTAAATCAACACCTAGTAATTTAATTGTATCTAATGGATCTTTTGAACCACCACTTGATAAGAAATCAAACACTTTTTGTTTTGCACCTGTTACTTTAGTGAAAACACGATGGCCTGAAATAATTGCTGCAACTTGACCTACTGCATATTTATATACATAAAAATTACCTACATAAAAATGAGGAATACGAAGTGGAGTAATGCTTGATAATGAATAAATTGAATTTAGATCCTCTACAATTGGTTTGTTAGTATATTCAAGTTCTAGTTTTTGATATTCTTTCATAACAACATCTGCCGTAAATGGTGCCCCACTATTAATTAATTCATTAGCAATTCATTCAAAATTTGAAAAAATTACTTGTCTTGTTGTTGTTGCGAAGAACCCACTAATCATTTCATCTAAAATCATTAATTTCATTTCAAGATCGTTCTTGTATTTTTCTAATAAATAGTAGTTTAAATAAACTTCATTTGAAATTGATGCAATTTCCGCATAGAAAATCTTGTAATCACTATAAATTTTTTGTGTGCGATTAGAATATAACGAATGCATTGAGTGGCCTAATTCATGAACAATAGTTTGTACATCACGTAATGAGTTAGTATAGTTCATTAAAATATAATATTTACTAATACCCTTTGTGCCACCAATTGAATAAGCCCCACCACGTTTCCCTTTTTGTGGTAATCATGAAACTCATTTTTCATCGAATGCTCGTTTAATATTACTTACATATTCATCACCTAAAATTGATAATGAATCAATAGCCATTTGTTTTGCTTCTTCAATTGGAATGTCAATTTTTTTAGAAATTAATGGTAGTCCATTATCTCAAGGTTCGATCTTTGAAACTTTAATTAATTTTTTTAAATATGTATTTCGTACTTTTTTATAATCTTCATTTGTATCTTTATAAAGTTTTACTTGATCATAAATTAAAGTAATTAATGATTTGTCAACAACATCATCAAATGCTGCTTTAGCAATATAATCATCAAAGTTTTTTGCTTTTGCTTGTTGATTTAATGATAAATAGTTGTAGTACAACATTTTTGTAATTGATTCACGTGAATCATAATATGCTCTATACATTGATAAATAAGCGCTTTTACGCAAAACGCGATCTTTTGATTTTAAATGAACAAAAGCTTCTGCTTCATTCTTAATTGGGTGTTTTTTACCTTTTGCATCAATAGCATCACTAAATTTCATATCATTATTTGTATATGTTGAAAAAATTGTTGAAAAACCTTCATCAGCACGTATTAATGTTGAAAATAACTTACTTTGTTCATTATTTAATGTGTGTGGTTGATAACGAAAAATTTCATTATACTTGCGTGTATATACATTTAATTGAGAATCTGTCAAATATTCTTTAATCTTTGCTTCGTTTGCTAAAACTACACTATTATAATTACTTAATGCTGTTTCTAATTCATAAAAAGAAGCTGATAGTTTTTGTGATCAAGAATTTCATTTTGGATCAACAACATTAGTATTTAAATTATTTGATACATAATTATAAAAACGATTTGCAACAATTGTGAATTCTTCTTCTAAAATTTGTCATTGAGCAAAATTTTGTTTTGAATCTAAAAAAGTTGGAAAAGCTTTAATGTATTTAGCTTTTTTTTCTTCAAATTCAGCATATAAATCATCTAATGATTTGTTGTTTAATAAATCATCAAGATCTCATTCGTATTTTTTATTTTCCATTACAAACTCCTTATAACGAATATAATAATTATAATATTTATTAATAGCAATATATAAAGTTAATTGCTAAAAAATAGGAAAAAAATCATGTTGAGTTCTATAAATAAGTTAAAAAATAAATTAAAAAATCTAGCAAAAAAACCAATAAAAGTTTTATTTATTGAACAAAGTTTAGAAATTGATAAAGCAAAAAAAATGTTAAGTCAAGATACTTTAATTAATATCGTAGATTTGTCATCTTTTAAAACACCAGAAATTATTAATGAATGCGAAGAGATTTGATATGAATTACGAAAATTAAAAGGTGAGAGTCGAAATGATGCCCAAATAGCAGTTAATGACAATTTAAATTTTGCTGTTTGTTTAAGTTATTTAAAAAAAATCGATGTTTTAATTGTGGGTGCTAACTTGTCATCAAAAACTTGTTTTAGTAGTATCTTAAAGATTTTGAAAAATCCTAACGGTTCGATCGCATATAGTACAATGGTTATGATTAATAAACGAGAATTAATTTTTATGAGTGATTGTGCTTTAAATTTAAACTTGAATAAACAACAAATGATTGATGCAGCAAAATATTCATGCACAATTAGTAGAAATTATTTTGATGTTTTAAACAATCATGTGGGATATATTAGTTATTTAAGTCAAAATGATGTTATTTATCAAAATAAAAATTTTGATTTTATTGGCCCAATTCAATTCGATGCTGTAGTTGATTTAAAAGTTCGTAATAAAAAAGTACCTAATATCCATCAATACCATATTAATCATTATGTTTTTGATAATATAAGTATTGCAAATACTGTTTTTAAAATTTATCAAACAAAACTTAATTATTTAACAGTCGGTACATATATTAGTAATATCTTAACAAAAGTTAGTGTTATTAGTCGTTCAGCAAGTAGTGATGAAATTATTGCAACAACTTATTTATTATGCGTGGCTTTTTTAACAGATTTAACTTAAATTAAAAACCACTAAAGCTAAGTATTTTATACTTTAGTGGCTGATTTTAATTAATAATTAATTTATTAGTCTCTTCATAATCAAAATATTCTTTGCTAATGTGTGTTAAAATTAATAATTCTACTTTTAATGATGATGAAGTTAATTTATTTAAAATAATTGGTGTGTTAAATTTAATATTTTCATCTTCTAAAATTGATTTTAATAAATTAATATTCTCATAATTAATTTGTGTTTTTACATTAAAAAAATCATCAAAACCAGAAAGAATTAATTGTTTACTAATATTAATGTTATTAGTATCGAATTGTTCTAAATCCATATTATTTGGTGTTATATAGTATGATACTGCAATTATTTTTTTATTTTTAATTAAACTAAATTTAGTGATTGAATTAAACTTATTACCATGATATTCAAAATCAATTAGAAATTGTTCGTATTTAATATTATCAATGGTTTTGTATAAATTAGCAAAAGCCTTGTTAGTAAAGTGATCAATACTTGAATAAACATAATAACCACTTCCTTTGATTGTTGTTACAATGCCTAAAACTTTTAATACTTCATAAGCATTATGAATAGTGATTCGTCCACAATTAAAATAACTTGCTAGTTTATACTCACTAGGAATCAATTCATTTATTTTATATTGATCCATAGCTATTTTTAAGATAATATAGCGAACAATGTAAGTTTTATTTGTCAGCGTTTTGTTTTGATTTGTTATTGTCTTGTATTTCCTTTATCTTTTTATCAAGTTCCATTTGTTCAGCTATTTTCTTACGAATATTAGCTTCAAGAATTGATAAATCACGATCAAATTTATCAACAATAAACGCTGTTGTTTTTTCTTCTAATAATGTTGATTTTGCAGCTTCTCATTGTGCTTTGTTTTCTTTAAAGTCACGAATTGATAAGTTTGTCTCTTCATAGTATGATTCAAGTATTTTTGTTAATTCTTCATCTTTAATTTCAATTGTAAATTCTTTTTGTAAATCTTGAAAAATTAGTGCTTTAGCGATGATTTTTTGTGCAATTTCATAAGCTAACTTTTCATCTTCAACACCAAATGCTTGTTTAATACGTGGTGCTAAATCTTTAATATCTTCTTCATTAACATCAATTTCATAAAAATCAGCAAGATATGTCATTGCTTTATTAAACAAATTTTCACGAACAATAATGTTATGTAATTGTTGAGAACGTTGTTCTTCAGTTTGTTTTGCAAAAACTGTGTTAATTCTTTCTTTATGCATTTCTAATGCTTTAGGATCAACATTTAATTCTTCTAAAACAATTGTGTGTTTCCAATCAATCGGTTTTTTATTAACGATGGTACTTTTAAAATTCATATATTATTTCCTTTTAGTTATCAAATAAATCATGAGTTTAATACACGTATATTATAATATTTTAATTTATTTACTTTAATTACTAAAATATTATTCACTATTTGATAAATAATCTTTAACTTTAATCTTATGAACAATTTTTTGATTATCAACAATATCAATTTGAGCATCAATGTTATAAACGTTTTTTAATAACTCTTTAGTAATAACTTCATTTGGATTACCATTTGCATAGATTTGCCCATTTTTTAAGACAAAAACTTCATCACTATATTGAATAGCTTGATTAATATCATGTAAAATTGCAATAATTGTTTTTTGACGTAAAATTTGTAATTTTTTTAGTGATTCAAGTAATTCATATTGATTTTTAATATCTAAATATGTAGTTGGTTCATCTAATAAAATAATTGGAGTATCTTGAACTAAACACAATGCTAAAATAACTTTTTGGCGCTGACCACCAGATAATTCTTGCATAAATTTTTCTTTTAAATCGTGCAGTTGCATTTCATTAATTACTAAATCAATAATTAATTTATCTTCTTTTCCTAAAATACCACTAAATCCTAAATATGGATTACGACCAAACGTAATAAAATCAATAACACGAGTGCCTTGGGGAATTTCAATACTTTGAGGAATGTAAGAAATTAATTGTGCTAATTGTTTAAAACTTAATTCATTAATGTTTCTATCAAACACTTTAATAATATTTTTTTTAAGTTTTAATAATTTAACAATCGCTTTCGCTGTTGTAGACTTACCACATCCATTTGGTCCTAAAATTGTTGTAAATGAATTTTTTTTAATACTAAATGATAAATTTTTTAAAACTTCAGGAAGTTTTTTGTGATATCTATAAGATAAATTATTAATAATTAAAGCGTCGTTTTTACTTGCATTAATAATTTGATTCCGATCTAATTGATGATTGCAAAAGTATTTAGCAAAAATTTTAAACATATTTTTATTTTCCTCTCACAATTAGATACATAAAATAAGGTGTAATAATTGTTGTTGATAAAAAACCAACTGGTACTTGTGTATTAAAATTAACCGAAATTAATAACGCAAAACACACTAAAGTTGCGCCGATTAAAAATGCTGCAAACATTGTAATACCTGTTCTATTACCAAATAATTGACGTGCAACGCTAGCGCCAATAACTCCTAATAAAGCTATATAACCAACTAAAACAATTTCTAATCCTGCTAATAAAGCCACTAGTAAATAAATACTAATTTTTAAACGCTCAATATTAATTCCTGTTGTTGATGCTAAAGCAGCGCTTGTTTCGTATAAATCAATTTTTTTACGTAAAAATCAAATTGGAATCAATGTTAATGCAATTAATACTGAGCAAGAGATTACTAATGATAAATCAATATATTGCGAATTTGGAATAATTCCTAAAGTATAATTTTTGATATTTTCAAAAGCAATCATTAAATTTTTATCTTGATTGGAGAACAATGAATTATTAGCAGGATTAATTAAAACCACGGAAATCGCTTCAAATAAAAAATTCAAAGCAATTCCGATAATAATAAATTTTTCATTATTAGCTCTTCCTAATTTTGATAATGTATAAACAATACTTGTTCCAAAAATTGATGATAAAAACATCACCAAGGGTAAAATTCGCATTTCAGGATTTAAATAACTGCTTGTTTTATCTTTAATAAGAATTAAATATACTGTCATTAAAATGATGTTTAATGAACCAATACCTAAAACTGACGTATCTGCTAAATGATTACGTGTAACTTTTTGTAATAATAATCCAGCTACAGCAATTGCTCCTCCTGAAAAAAAGGCCATTAAAGTTTTAGGACCACGAATATTAAAATTAAATTGATTGATCGATGCTTCAAAACCATTAGTGGTATCAAAATAAATTGCTAATGTTAAAAAAACAAAACAAATGGCAATGAGAATTAGTCCTGCCATTTTAATGATTACAGATTGTTTATAATTAAGATTTTTAATTAAATAAAAATGCTTTTGCTTTAAATTCGTTTTTTGATTAATTTTCATTGATTCTCGTTTTTTTTATTATTTTTGGTTAACAAATGTTTTTCAATATGAACCCCAATCATTAGCTTCTTTTAACAATTGTTTATTTTGTTGGATTAATTGCGGATCAATTGTTTTATTAATAATTTTAATCACAGAATCTAAAATCATATTATATCCAATAGGTGATTTAATTCCATCATTTCATAATTCATAATTATCATCAAAAATACGCTTCGCTGGATCATCATAATCTGATTTAATTAATGACATGATTAATTCTTTTTGTGGTCTTGATAAATTATTACTATGAAAATCTTCTAAGTTTTGATAACTAATTTTAATTAAATAGTCGAACTCTGAATTAAATTTAGCAAAGTTACTAGCACTTAATGATCCCTCTCCTAAATTTCATCATCAACCATTATCATCCATATTAAATAGAGAACCAACAATGTTTTTATCAATTTGTGTCCCATCTTGATTATCACCACCAAAACAATGAATTTGTCCATTTCCGTGTCTATGACATAATAATTGTTTGCCGTTAACATCTTCTAATAATTCAGTCCATTCGTCCTTTTTTAAGATTGGTTTAGGTGCTTTAAACCCTAAATCATAATATAAAAAACCGTATTTAGATTGAGTTTGTAAAGTTGAAGAACCACTATTTTGTGATGTAGTTAATAAGCCTAAAGTTTTGGAATTAGTATTATCAAATTGTTTTATATTAGGATTATAATTTTTATCAACAACGCCAATTGCTTGAAAATACAAACGCATTTTGTTTAAGCGATTAATTAAATTATTAGCAATTTGATTAGCATTAAATAACGCTTTTTTTAATGGTTCTAATTTTGCTAAATTATCTTTAAAATCAATTTTTGCTTCTTCTGAGTATGCATATTTTGTTGTTGCATAAGCAATATCATATAAATAAAGCAATGAATCGCGCATTGTTCCTAAATAAACCTGTGTACCTCCATCTCATCGCATATTTTGTTCATAAATAGGATGGTGTTGTAGAATTGATGTAACATAACTATCTTGTTTTGTAGTTGATAAAAAAGGATCATAATTTAATTTATTTAATCCTGATTCCCTAACTTTTTTTGTTAACACAACTGTTTTAAATGCTTCATAAATTTTTGAATTTAATGATTTAGGATCTTTTCATTTTGTTTCAGTATAAAAAATAGGTATATCATTACCTTCTAAAATTCTTTTTTTAAAAGCTGAATTATTAAAATTAATACCATCAAATGTTTCAGCATTAAAATAGTTTTTGTTTAATTTATCAGCAAAATAGGAATAATTATAAAGTTCGTTTAATTCACCTTTTTCTTTATCCTTATACTCTAAACCTAACAATAGACTTTTACGTAATAATGAGTTAGGATCGTTTTTAAAATTTAAAATGTCATTTTTATTTAAGGTTAAATAAAAATCACGACTCATTTTAAAATCAACAGGAATAGCAGTTTTTTGATTTGATATCGGGCCACCATGTGCAACTAATGTTTTTTTTGCACGTTCAAAATGACTATTATCAATCAAATAACGATCATAATAAACAAGTTTGCCTTTATTTTTATTTCAATACTCTTCACTTACACCTTCGCCATAAGTATTGCCACTAAAGCTTCATCATGTAGTTCCATATTGTTTAATATTTTTTTCTAAATTAATTAATTTTTCTTTAAATTTATTAGCAAAATCGTCTTTTTCATAAAATCATTTTTGATAATTTGGTTTTTGGGGATTTAAATAATCATAAGGTGAATTTTTCTTTCAATACATTTGTTGAGGATAATAATCAGGTGTAATTCCTAACGCTAATAAATTATCAGCCTGAGAAGTATAAGTTGGCATTATATCGTATTTGATTTGCTCTTGAACGTTTGCAGATGTTTTTTTTGTACATGAACTAATAATTGTTGTTGTACCAATTACAATAGAAATTGTTGCCAACATACTAATTAAATATTTTTTTGTTTTCTTATGCATTTTTAGATCCTTAATTTTATTTATTTGAATTCTTATCAATTATAAATAAAACAACAATTATGGATATAATTAAATTAATTAAATATCTAATTAATCAAAATTAATAATAAAAAATGGAATTAATAAAGCTTTTTTTAAAAAATAAAAGTTTTTAATTCAATTAATATTTATATATAATAAAATAGAAGTCAAATTATGACTTGATAATAATTTTTATAACAAAAGGAAACTTATGATTGAAGATTCCCAGGAAACGATCAGTACTCAAGCAAATTTTAGCGTTGGCATTTTAGTAATATTAGCAATAATTACTCTTATTTTGATATGTTTTTCAGTCTTTTTTTCAGCAGCCGAAACTGCTATTACCAGTGTCACAACCGTAAAATGAAAAACTGAAAGCAATAAAATTAAATCAAAAACAATTACTAATGTTGTTTATAAAATGATTAATAATTATACAATTACCTTGGCAACCATTTTAATTGGTAACACATTAGTAAATACAGCATCATCAACAATCGCAGGTTTATTTTTTGCAGGTATAATGAAATCTTTTAGTACAACAAGTAACGCTGAAGCTATTGGTACAGGAATTGCAACAGGAATTATAACTTTAATTGTTTTAATATTTGGCGAATTTATTCCTAAAAATTTTGCTAAGAAAAATCCGATTAAAGTTCTTAAATTAGTAGCTTATCCCTTAAATTGTTTCTATATACTTTTTTGGCCATTAACTTGATCTTTAAATCGAATTTTTAATAAAAAATCACAAAATATTATCACAGCCACTGAAGAAGAATTAAAATCGCTTATTGAAGTTATTCGTCATGAAGGAACTTTAGATAGTCAAGAAGCATTAATTATTAATAAAGCAATTTTATTTGATGATATATTAATTAAACAAAAAATGGTTCCATGAACACAAGTTGTTAAAATTAATGAAAATAAAACTGTTCTTCAAAGTTTTGAAATTTTTAATTCAACTGGATTTTCGCGTTTGGTTGTAGTCAATAAAAACGATGAGGTTGTTGGTATTATTTATTTAAAAAAAATTATTAAATATCTTCTTAACGAACCTAATAAAATCATTAGAACTATCATGCAAACACCTTTATTAATAAACCAAAAAGATACATTATATGATGGTTTAAGAACAATGCAATTTCAAAGAATTCATTTAGGAATTGTTGTTGATAATATTACTGAAAAAAATCCTATAGGAATCATTACATTAGAAAACATTACTGAAGAATTAACTGGTAATGTTTATGATGAAACTGATAATGAGAACGAAATAAAAGAAATTCAAATCGTTAATGAAACAACTTGACGTGTTGATGCTAAAACTAACGCTAAATTATTTTTACATCAATATATTGACAAAGATATTGAAGTTAACGATGATATTAGTATGGGCAAATATATTTCTAAAATTAATAAAAATCGCGCATTTAAAAACCGCCAATTAATTAACACTCCTAATATGTATATTGAAACTTTTAAAGATGTAAGAACAAATCAATTTGCCTTTTTAATTGAAAAAAAGATTAATCAATCCTAAACAATGAGATAAAAAAACTAGTTTTTAAAAACTAGTTTTTTTAATTTTAATTCTTCATACATTTCTTTAATACGAGAATTAAAATGATTTAAACCACTTTTTGAAATTTCAATATTAAACATCTGTTTTAATAAACAAGAAAGTTCATTTAATGAACAGTCTGGATTTTGTACTCGTAGTTCACAATACTTTAAAAATTTAGTACTTTGTTTGCAAAATTCATCGTGGTTTTTTTTTATTTCTAAAATCATTTCTTTAGTCAAAACCCCTGCTTTAGAACTTTTAGAAAGGTTAGAAATATCTAAATTATTTAAACGTTGTTGTTGATTATTAAAATCACGTTGGATACGAATATTCTCAAATTCAAATAACGAATTTTGCGTACGGATCGCTTTTAAAAAATCACTGATTATCTCTGATTTTTTAAAATAAACAATGGTTTTTTTATTACGATAAATAATTTTATATTCTAAACCATAACGGGCTAAAGCCTTTGTTAATAATGGGATGTAATTATTTGAATCACAGCGAATTTCTAAATGATAGTTTTCAAGTGGGTGTGCAATTGAACCACCAGACAACATTGCACCAATTAAAAAATTTGAACAATAGCGGTCTTTATGAAAATTTTCATTATTCGCATCATCAAATAGTTTTAAAATTTCATCTAATTTATCCAAATCACCTCAAAGTAATAGCGAATAATTAGTACGAGTTTTATTTAAATGGTCAGGCTCAAACATAATCTCATATTCAATATTTAAATTATTAAAATAATCTAAACCATTTAAAATAAATTCAATAATTTGTAAATTTTGAGATTTAATAATTCAAGTTAGTTTTTGACCAATTTTAATGGTTAAATTATTACGAAAAAACGAATATAAAATAACTTTATAGTCTGTGGATGAATAATCAGTGTTAGTATAAATATCTTCTTTAACAAATTCACTAAACGTGAATTTGTTAGAATCATTATCAATTATTTCTTGTAATGTACTCTTTGGCATAAAGTGCTGCAATGACTCCATCATTTGTAGCAGTCGCAATTTGTCGAACATTTTTATCAACAATATCACCGGCTGCAAAAATACCACAAACACTAGTTTGCATATTTTTATCAGTTGTAATAAATCCGTTTACCTCATTAAAAATACATAAATTATTTGTATTAATTCTTGAAGGCAATAGGCCAATAAAAGTGAAATTAGCTTCAATATTTAGTGTAGTTACTTCGTTTGTGATTAAATCTCTAAATTTTAAACCTTCTAAAAACTGATGACCAAAAAATTCGAATGTTTGTTTATTGTAATAAATTTTTATATTAGGTGTGTTATTTGCAATTTGTACTAATTGTTGTTCTGCTTTAAATTGTGGTTTATTGGCAATTAAATGAACTTCTTTAGCAATTGTAGCTAAATATATGGCCTCTTCCACAGCAGAGTTACCAGATCCAATCACAGAGACAGCTTTATTTTTATAAAGTGGTCCATCACAAATAGCGCAATAACTAATTCCCTTGTTTTCAAATTCTAATTCACCTAAAATGTTTAACCGTCTATTTTCTGTTCCTGTTGCAATAATTACAGTTTTAGTAATTAATGTTTGTGTAGTTAATTTAATTTTTTTATATTTTTCTTTTAAAGATATTTCATCAGCAGCTTCATAAATAATAGAAACATTTAAATTTAAGACTTGTTCGTACATTTTATAAGCCAATTCAGGTCCTGGAATACTATTGATACCTAAATAATTTTCTACATTTGATGTTAATGCTATTTTTCCTCCTGGGAATTGTTTTTCAACAATGATCACATTTAATCCAGAACGTTTAGCATAAACAGCAGCAGCTAAACCGGCTGGACCTGCGCCAATGATTACTAAATCGTAAACTTCTTGATTCATTTAAAATCCTTTTCTTTATTATTTATGACAATAAAATAAAAATTCTTCTGGCTTTCTTAATTCTCCAAATTTATGAATTTGGTCTTTCTTTTTATAAAACATTTCAATAATTGCATCTAATAAGAATTTTTTTCTTAAAAGTGGCGCTATAAATTGTACATATAATGCACCTAATACACCAAAGATTAATAACAATGAATTAATTATATAAGTTCCTTCAAAACTAAACTGTGGAGCACGAGCTGACTCCGTACTAAAACGAGTAACACCATAAAAGAAAAAATATGACATTGAAATAAAGCCTATTTTTAATTGTTTAATGTAAGACAAGCCAAAATAAATAAAAACAAAAACAATTACATTAAAGAATGATTCATATAGAAATAAAGGTTGATAAATTGTAAATAGAGTTTTATTGCCCTCAATGAAATAATGTTTCATTCCTTCGAAAACAGCTGGCATAGCTTTTTTTAATCATTGTAAATCGTTAACAGTACTTTCAGCACCAAATATTTCACCGTTAATAAAATTTCCTCAACGCCCTAATGCCTGACCAATTAAAATTGTTGGAATAATAGCATCCGCATATATTCACATACTAGGTTGGCGAATAACAACATTGCCATCAGCATCAATATCTCGAATGTGATATTTAGGCATGCGCAAAATAAGAGGAAAATAAACTGCAGCACTTAATACCCCAGCAATCACCCCGCCTTGAATCGCTAACCCTCCACTTCTAAAATCAAAAAAATCTTTAGCATCACCGATTGCTAATGATCATAAACGAGCACCAATAATTGTCATGGGAATTGCTAAAAAAATATAATAAAATCCTGGATCATAAGGCACTTTATAATGAAATTTTAAACGTAGACTATATGTTAGTATAGCAATTAAAAAACCGCATACAAAGATGATTCCATATCAATATATATTAAAATTGCTACCAATACTAAAAGCTACTCGATGTGCAACAACATCATTAACTAATGTGCTTTCTGGATTAATAATTTCTAATTGCATTTAAATCCCCTTTTTATTAATTTGTAAAATAAATTCTTCAGCTGAATTATAACCTGATGTTTTTAATTTATAATCAGTAATGGCTGTCTCAATGATATCTGAAATACTTCTTCCTTCGATAACCGGAATATAATAATGGGCTATTTTTGTTTCTAAAAATTCTTTGTGTTGAATTTCACGACCAAAACGTTCAAACTTGATTTTTTCATGTTTAGCTGGTTTTATTAATTCAATAACTATTTCAATTTTTGTTGATTCAATCATTCGTTCAATACCGAAAGAACGTGAAAAATTCATAATACCTAAACCTCTAATTTCAATAAAATCTTTAGTCGAATCTTCAGCTCTTCCATATAGATTACCTCCAATTCTATTAACTAAAATAGCATCATCACCTACAAAAATATGACCTTTTTTAACTAATTCAATTGCTAACTCAGATTTACCAACACCTGATTCCCCCATTAAAATAACGCCGATTCCAAAAACAGATAATAATGTTCCGTGAACTAATGATTGGTGTGAAAGTTTTTGACTAATGTAACTATTAATTGTAAAATTTAATTCATAAAAACTATATTTAACCTCTACAATTGGAATTTTATAGCGCTCTGCTAATTTTAACAAAATTCCAGCATGTTTAAAATTTTTACCAAAAATAATTAATGGTGGATTTAAATCTAAAATTTTTCCTAATTTTTTAGAAATGATCGTTTCTGAAAATTTATTAAAATAATTGCTTTCTTTTGAACCAAAATAAACAATATTTCAAATCTCTTTAAACAAAATTTCGCCAGCTAATTCAAATCCAACACGTGTAATTCCTGTTGTAGAAATCTCACGATCAATATAGTCAGAATTTGCAACAACGTTTAAATTAAACTTTCTAACAACTTGACTAACAAATAATTTACCACGAATTTCCATTAATTATTATCTACCTATTTCTCAATAACATTATGATCATCGATAATATCAACATTAGAGTTGCTAATATTATCGCTTTTATTGTATGATATTAAACTAATTAATTCATCATCATTAACATGCATATAAATATTTTCTTGAACACGATAAGCTTTTTCTTTATTAATTTGTTTACCAAAGAATTTGATTAATGAAATGATTTTATTTTCACTTGGATTAATAATTTTATGATCAATTAAAAAGTAGTAAATTAAATAATATTGAACCATATCGTCTTGACGAATAAAGTAGTCATATTTGTATTTATTTTTTTTAAATTCAATATATGATAAATCTATAAAATTAGCAAAAGAACTATGTTCTTTATTATATTTAATAACGAATTTTTTAAAATCATAAACCTCATTTAAACTTCAAGAACTAATTATTTTACGAACACGATTGCGTTCGTATATATCTAATTCATTTGTTTCGTCAATACCATACTTAATTTTATTTTCATCACAATAGCGTTGTAATGTTTGTTTACGCACAAATAAAACCGGACGTTTAACAATTATGCCATTAATAACATTACTTTCCTTAATGCCATAAAATAAGGCTTTTGATTGGCGAGCTCGTTGCATATATGCCGTTTCTAAAAAATCATCAAAATTATGCGCTATATACAAATTTTCAATATTATATTTTTTACCAATTTCTTTGAAAAAATCATAACGAATTAAACGTGCTTTTGTTTGAAAATTATCAATATGACCATAACGTTCATAAACTTTTTCATCAACATCTAAAATTTCAATTATTAAATTATTTTTTCTACAAAAATCGACAACTATTTGCTGATCACGATCGGATGATTCGCGTTTATGATAATTCACATGACAAACAACACGAATATTTCTTTTGTACATATTTAACATTGCCATAGAATCTGGACCACCAGAAACAGCAGCTAAGTATTTTTTATTACTAATTCTATTAATTAAATTTGTTCATAACTTTATCAAAATCTTCACCAATCGCTGCTAATGTAGCTAAAGCGCCCTTTATAATAGCTGGTTTAATTATAACTAATTCGTCAATCTTAAAATTCGATAAGACATATTGATCTAATTTTATATGTGGATCTTTATCAATTCCTATACGAATGCGTTTAATTTCATCTGTTTTTAAAATGTTAATAATATTTTTAATTCCATTTTGCCCTCCTGAGGACCCCTTTTTTCTCAATTTTATCGTTCCTAATTTCGTATCAACATCATCATAAATAACTATTAAATTTTTAATTGGAATATTATAAAAATTAAGCATTTTGATAACAAATTCACCACTTAAATTCATGTAAGTTGTTGGTTGACAAAAAAACACTATGGAATTGTTATAAATTGTTTTAGTAAAAATTCCATTAAATTTTGAACTATCTAGAGACAAATTAAGTTTGTTGCAAATCTCATTAATAACCATAAAGCCAACATTATGTCTTGTTTTTGCATAGTTTGATCCTGGATTACCTAATCCAACAATTAAATATTTTTCCACATTTAATACCCATATTATAAACGTACAATGTTTATAAATCTCTTCAATAAAATTATTATATAATAGATGAATTTTAAGTTATGCACATTTAAACACTTAATAATAATATTAATAATTAAAAATAAATAAATAAAAAATAAATTATATTTTAAAGCATCACGCTTTTTTGAATTTATATCTTTTATAATATAAATAATAAAAAATGAGGATTTTAATAAAACATGGAAGTTTTTATTTCTATAAAAAAATTAATTGAAGCAATAAAATTTAGTACAACAATTGCTAATACAAACAATGCAAATGCTTTATTATTAGGTGTTTTGATTGAAGTTAGTCAAAATAAAATTAGTTTTAAGACGACAAATAATCAAGTGAGTGGTTATAAAGAAATTACTGAAGGATTTGAATATTTTAATAGTGGAAAAGTTTTAGTTACAGCTAAGATTATTTTAGGATTAATTTCTAAGCTTAAAGATAAATCTGTTTTATTAAAACAAATTGACACAAATATTTTATTAATTAAAACTGAAAATTTTGAAACTCAAATTAATACAATGAATATTGAAGGCTTTCCTAATTTAAACTTTAGTTTAGAAGATTACGTTAAAATCAGTTTGCCGCATCAAATTATGCAAGAGATTAATACAAAGGTTTTACCCAACGTTTTGAACAGTCAAGGAATTGAAAAAATTCAACCGATTTCTGGAATTTTGATTGATACTCAAACTTTAGAAAATAAGTTGATTGCTATTGGTACAGATAAAATTAAAGCTTCATGTTTAATAAGAGAATATATGGGTGAAAAATTTAAATTTATTGTCTCTTATTCAACAATGAAATTGATTATCGAAATCCTTAAAAATGTTGAATATAGTAATAATCAAACTGTTGATTTTTATGTTCGAAGTAGAAGTTTAATTTTTAAAATTAATGATGCAATTTTACAAACAAGAATGATTGATGGTGTTTATCCAAATATTTACTCAATTTTTGATGAAACAAATGAAGAAAATACATATGTTTTTGATCGTCGTTTATTGATAGAAATTATTGAACGGGGTATGAATATTGTTATGCAAGAACAAAACCCTAAAATTAGTATTAGAATAGACAAAAATGAAGCAGAAATCAGTTTAACAACTTTTGAAATTGGTAATATGAAAGAAAAAATGTCAATAGTTAATTTAAACAATAAAAGTGCTGAGTTTATTTTAAACCCTAGTTTGCTAGCTCATGTTTTAAAAAATTTTGATAACAACGATGTAACTTTTAAAGTTAAAGATGAAATTTTAAGACCTATTATTTTTATCGATACCAAAGATGTTGGATTTAAACAGATTTTATCAAGAATAAAAAATTAAAGAAAACAGTTTATTATTATTTAAATATTAATATTTTTCTAATAAATATCACTTACATTTACTAGTTGTTTAACCAAATTTTTAATATTTTATATATGTTATAATAAATATGTATTACTACAAATATTTTAAGACCTATGAAAAAAATTTTTATATCAACAGAGTATGTGACTTTAAACCAATTTTTAAAAATAGCTGGATTGATTAATAATGGTGGTCAAGCTAAATTTTGATTGTTGGAAAATAAAGTTATGGTTGATGAAAAAATAGAAAATAGACGTGGTCGTAAATTATATGATCAAATGATTATTAAAATAAAAAATCAATTGTATCAAATAGTTAAAACTAATGAGTAGATAGGAGTTTTATGAACGATTCTAATAAAGAAAAAAAATACACCGCCGAAAGTATTAAAGTACTAGAAGGTTTAGAAGCAGTACGAAAACGTCCTGGTATGTACATTGGTTCAACCCAATCAGAGGGTCTACACCATATGATTTGAGAAATTGTTGATAATTCAATTGATGAGGCTATGGGCGGATTTGCAACAATTGTTAGAGTTATTCTAAAAAAAGACGGATCAGTACGTGTAGAAGATGATGGACGCGGAATTCCAATTGAAATTCACGAAAAAACAGGCTTATCAGGTGTTGAAACAGTATTAACAGTTTTACATGCTGGTGGTAAGTTTGATAATGATAGTTATAAAGTGTCTGGTGGATTACATGGTGTTGGTGCCTCTGTTGTTAATGCTTTAAGTAAAAATTTTAAGGTTTGAGTAAACAAGAATCATGTTCAATATTATGTTGAATTTATCAATGGCGGTCATGCTATTGAGCCACTAAAACCGATTAATAATAAAGATGTTAAAGAAAAAGGAACAACAATTGAATTTATTCCAGATTTTGAAATTATGGAAGAAAATGAGTGAGATGAGCTTAAAATAATGGCTCGTTTAAAGCAATTAGCTTATCTTAACAAAGGTGTTAACATTGAATTTGAATCAGAATTAACTAACCGTAAAGAAAAATGACATTATGAAGGTGGTTTAAAAGAATATATAACAGATTTAAATGCTGAAAAAGAACCATTATTTGATTTAATTGTTTATGGAGAAGAAGAAAAAGAAGTTAGAGTTCCAGGACATAACGAGCAAACTTATAACATTAAATGTGAAGTTGCATTTCAATATAATAATTCATACAATAACTCAACCCATTCATTTTGTAATAACATCAATACCACTGAAGGTGGAACACACGAAGAAGGTTTTAAATTAGCGATTACGCGTTTATTAAATAAATATGCGGTCGATAAAAAATATTTAAAAGACACTGATGATAAAATCACTAAAGAAGACGTTAGTGAAGGATTAACAGCAATTATTTCAGTTAAGCATCCTAATCCTCAATATGAAGGTCAAACAAAAAAGAAATTAGGTAATAGTGAAGTTCGTCCTTATGTTAATGAAATCACATCTATTATTTTTGAAAAATTTTTAAATGAAAATCCTGAAGAATCAAAAAAAATTGTTGCTAAGGTTATGCAAGCTGCTGAAGCACGACGACGTTCACATGAAGCACGTGAAGCTACACGGCGTAAATCACCTTTTGAATCAAATTCATTACCTGGTAAATTAGCTGACTGCTCAAATCGTGATTCAAGTGTTACTGAAATTTATATTGTCGAAGGAGATTCAGCGGGTGGTTCAGCAAAAACAGGTCGTGAGCGTGAATTTCAAGCAATTTTACCACTGCGTGGAAAGATTATTAATGTTGAAAAAGCTAAAATTGATAAGATTTTTGCTAATGAAGAAATTCAAAATATGATTACTGCTTTTGGAGCAGGAATAGGTCCTGAATTTAATATTGAAAAATTAAGATATTCAAAAATTATTATTATGACTGATGCGGATGTTGATGGCAGTCATATTCGAATTTTATTGCTAACATTTTTTTATCGATACATGTTGCCATTGATTCAAAATGGAAATGTTTATATAGCGCAACCACCATTGTATAAAGTAAGTTATGCTAAAACAATTAAATACGCATATTCTGATCAAGAGTTAGAAAGAATTAAATCAACAATATCAAATATTAAATATAACATTCAACGCTATAAAGGGTTAGGTGAAATGAATCCTGATCAATTGTGAGAAACAACAATGGATCCTAAAAATAGACTTTTATTAAAAGTTAATATTGAAGACGCTGCAATTGCTGATAAAACATTTTCTCTATTAATGGGTGATGATGTAGCTCCAAGAAAAGAATTTATTGAAAAAAATGCAAAATATGTAAAAAATATTGATGCTTAAAATTGAGGTATATAAAATATTATGGCATTAAAAAAACCAAAAAAAAGTCGTTTAACCACCGAAGAAATTAAGCAACAATTAGAAGGTTCAACGATTAAAGAACAATCAATTACAAAAGAAGTCGAAACATCATTTTTAGATTATTCAATGTCTGTAATCGTTGCACGTGCTTTGCCTGATGTGCGTGATGGATTTAAGCCCGTGCATCGCCGAGCTTTATTTGCTGCTTTCGAAAACGGAATGACACATGATAAGCCTTATAAAAAATCTGCACGTTGAGTTGGGGATGTAATTGGAAAATATCATCCACATGGAGATCAAGCTGTTTATCAAACTATTGTAAGAATGGCACAAGACTTTTCAATGCGTTATTTATTAGTTGATGGTCATGGTAATTTTGGTTCGATTGATGGTGATAGTGCAGCTGCGATGCGTTATACAGAAGCACGATTATCAAAAATCTCTTATGAATTATTAAAATACATTGATAAAGAAACAGTAGATTTTGTACCTAATTATGATGCATCAGAACAAGAACCAAGTGTTTTACCATCAGGTTTTCCAAATTTATTAACAAATGGAACTACAGGAATAGCTGTTGGGATGGCAACAAATATTCCACCACATAATTTAACTGAAGTTTGTCAAGCAATAAAAGCTTATGCTAAAAACCATAATGTTACTATTTCTGAAATTATGGAATATCTAAAAGGTCCTGATTTTCCAACAGGCGCTGAAATTTATGGTGATAGCGGTATTATTAAATATTTTAATACAGGTCGTGGTTCAGTAACAATTCGTTCAAAATATGAAATTGAAGATATTGGGCAAGGACGTGTAGCAATTGTTGTGACAGAAATTCCTTATATGGTTAATAAAGCTAATTTAATTGAAAAAATTGTTGAATTAGTAACAAACAAACAAATCGAAGGAATTTCTGATTTACGTGACGAGTCAAGTCGTGATGGAATTCGAATTGTTATTGAAGTTAAACGTGATGTTATACCTGAAGTTTTATTAAACAAATTATTTAAAACAACACCTTTACAAACAAATTTTAGTGTTAATAATTTAGCTTTAGTTAATGGTGTACCAATGGTATTAAACATTAAGGAAATGATTAAATACTATTTTGAACACCAAATTGAGATTTTAGTAAGAAGAACAAATTTTGATTTAAAAAAAGCAAAAGAACGAATTCATATTGTTGAAGGTTTAGTAATTGCTGTTAATAATATCGATGAAGTAATTAAAATTATTAAAGCCTCAGGTGATGATGATATTGCTTCAAAATCACTAATTCAACGTTTCGATTTAACAGAATTGCAAACGAAAGCAATTTTAGAAATGCGTTTACGCGCTTTAACAGGACTAAATATTGATAAATTAAAAAAAGAATATGAAGATTTAATATTAGTTATTAAAGATTTAGAAGATGTTTTAAACAACTACAATCGTCAAGTTAATATTATTTGTGAAAATTTAGATTATTTAATTGAAAAATTTGGTGATGAGCGTCGTACAGAAATTATGTATGGAGTTAGTTCGCATATTGATGATGAAGATTTAATACCAGTCGAAGATATTGTTGTAACAATGTCAAAACGTGGTTATTTTAAACGTTTGCCAATTGATACATATAAAAATCAACGACGTGGTGGTGTAGGTGTACAAGGATTAAAAACGTATGAGGATGATGATGTTGAAAAAATCTTAGTTGCTAATACCCATACAGATTTATTATTCTTTTCTGATTTAGGTCGTGTTTATCGATTACGAGGACATGAAGTACCATTGGGATCACGACAATCAAAAGGAATTCCAGCAATTAATTTTTTACCAATTGAAAAAAGTGAAAGCATTTTAACTATTTTACCAATAGATAATTATGATCAAGGTTCATTATTCTTTACAACAAGTAAAGGAATTATAAAACGAGCTAATCTAAGTGATTTCGAGTCAATTCGTGCTAATGGTAAAATTGCTATTACTTTAAAAGATGGTGATAAATTATTTTCAGTAATGCAAACACTAGGTAATGATGAAGTATTTATCGGCGCATCAAATGGTAATGTAATTCGTTTCAATGAAAATGATGCACGTGAAATGGGAAGAATTGCGACAGGTGTTAAAGGAATTAATTTAGAAAATGATGAATATGTTGTAGGAACAGGGTTATCTAGTCATGGAGAATATGTTTTAGCTGTTGGTTCTAAAGGATTAGGTAAATTAACTGACATCAATGATTATCGTTTAACTAAGCGTGGAGCAAAAGGTGTTAATACACTAAAAGTAAATGATAAAACAGGTAACTTAGTAAGTATTAAAGTTGTTAATCGTGAAGAAGAAGCTTTAATTATTACAACTAGTGGAAAAGTTATTCGTTTATCGATTAAAGACATTAGTGTAATTGGAAGAAATACATCAGGAGTTAAATTAATTTCATTAGAAAATAAAGAAGAAGTAAAATCAATTGCTATCTTTAAAAAAGAAGAAATTAATGATGAACAATTATTACAAGAAAATGATTACAATCTAAAATAAATGACAATAAAAACTACTTTAAAAAATAAAGTTATACTTTACTTTTTCTAAAAGTGGTTTTTATTATCTAAACTAATAATTATTCAATTTGAAAGGATTTTAAAATAATGAATGTGGCTTTAGAACTAATTAATTTACTAAAAGAAAGACGTTTAAGATTGAGTGTTTGCGAATCAGCAAGTTGTGGAGCTTTATCATCATCAATTGGTGAAGTTGCAGGTGCTTCAAGTGTTTTTGCAGGTGGTTTTATTAGTTATAGTAATGAAGTAAAAATTAAATTAGTAGGAGTGAGTGAAAAAACAATTTTTAAATATGGCGCTGTTTCTGAACAAACTGCTAAAGAAATGTGTTTACAAACAAATCAAAAGTTTAATACTGATATTGCAATTTCAATTACAGGAAATGCTGGACCACAGGGAAGCGAAAATAAAGAAGTAGGTTTATTTTACATTGGAATCGCAATTAAAGATTTTGCAATTGTAAAAAAAGTCACATTAAATTCAAATGAACGTACTTTTAATCGTTTTTCTATAGCATGAGAAGCAATTAGTTATTTAATTGAATTGATAAAAAAATAATTTTACTAATTCTTTAAATAGGATAGGTGAATAAATTTATGAAAGAAAATAATAATCTTGAGAAATTAGATCCAATTGCAACACTTGAAACAAAATTTGCTAAATCATCTTATTTTATTGCTGATGAAATAAAAGATGAAAAAATTAATGCAATATCTACAGGATCAATTCATATAGATCAAATAACAGGAATTAATGGAATTCCAGTAGGAAAAATTACAGAAATTTATGGTAATGAATCATCAGGTAAAACTACAATTGCTTTACAAACAATTGCTGAATGTCAAAAAACTGGTGGGACAGTAGTTTTGTTAGATCTTGAGGGCTCATTTGATATTAATTATGCCAAAAGTCTAAAAGTCGATTTAACAAAATTAATCATTACGCAACCTCAAACAGGTGAACAGGCTTTTGATATGATTGAAACATTGATTAAAACAAATTCAATAGATTTAATAGTTATTGATTCAGTTGCTGCAATGCTCCCAGAAAGCGAATATCAAGCAAATATGAATGAAGCATTGATGGGAGCACACGCTCGCTTAATGTCAAAAGGTTTACGTAAAATTCAACCATTAATTAATAAAAGTAAAACAGCCATTATTTTTATTAATCAATTACGTGAAAAAATTAATACTTTTTTTGGCAATCCTGAAATGACCACAGGCGGTAAAGCTTTAAAATTTTATGCTAGTTTGAGAATAGAAACCAAAAAAGCAGATTTAATTAAAGAGGGTATTAATAAAATTGGTATAAAAACAAAAGTAACAACAGTTAAAAACAAATTAGCACCACCGTTACAAACATGTTTTATTGATATTTTTTTTGGTTCTGGTTTCGATTATAATAATGAAATTATAGATTTTGCAATCCAATATGGCGTCTTGAAAAAAAACGGTTCTTGATTTTATTTTAATGATAATAAAATAGGGCAAGGGCGAGAACAATTAAAAAATACACTATCAAAAAACAATGAACTTTTTATACAAATTTCAGAAAAAACTTTGGAGTTTGTCAATAATGAAAAAAGCAATTGACAATAACTTGTAATATAATTATTATTGGTGATATAAGATGGGTTATTTAATAGCATTTATTATATTACTAATATTATTTGTATTGCTGATTACCATTGTTCCAGTTGTAATGGTAGTTTATCTTAAGAAAAAACAACTGAAATTAACTTTTGTTCCAAAAAGCCAAACTTCTTTTAAAAAAATAGTTCAAAAAACTAAAGATTTAGAAGAAGAATGTGAAGATTTAAATAATAAAAATAATGAATTAAAAAAAGCAATTAGTGATCAAAATTTACAAATTGATTTACTCAAAAAAAACAATGAAAATTTTTTACTAAATGCCACAAGTTTAACAGCTGAACAAGCGAAAAAAGAACTTTTTAATTTATTAAAAATAAAATTTAAAAAAGAGCTTGCTCAAGAATATGCAAAAATTAAACACGAATTTAATGAAGCCCAAGAAATTTATGCTCAAAACATTCTAGTAGAAACAATGGAGCAAATTGCTGAGCCTTTAATTGTTGAAAGATCTTTATTTAATATTGACATTATTGATGAAAATCTAAAAGGAAAAATTATTGGACGTGATGGACGTAATAAAGCAGTTTTTGAAAATGAAGGCGGAGTTGATCTAATTGTTGATCGACAACAGCCAATTGTAGGAATATCTACTCCAAATCCAATTCGTCGTGAAATAGCACGAATTGTTATGCAAAAATTAATTGATTCAAAAAATATTGATATTAATCGTATTGAATTACTTTTTAAAGAAGAACGTGAAAAGTTTGAAAAAAAAGTTTTTGAGATTGGTAAAAACGTCGTAGAACAAACATTAGGTTTTTTTGATTTACCTGAAGGAATTTATTCTTATATAGGGAGAATGAAATTTCGTAATTCTTATGGTCAAAATATTTTATCCCATTCATTAGAAGTTGCTGAATATGCTGAACGAATTGCTAAATTAATTAATATTGATCCTATAAAGGCAAAAAAAGCTGCATTTTTTCATGATATAGGAAAAACGATTGATTTTGAAAGTGACCTAGATCATGTAGAAGCGGGATTATTGATTGCTAAGAAATTTAATTTAGATGATTATATTTATAATGCAATTGAATCACATCATAACAAAGTAATTCCAACAACAATTTATGGTGCATTAGTTAAAATAGTTGATACGCTATCAGCAGCCAGACCAGGAGCACGTGTTAATTCTTATGATGAATATTACAGTCGTGTAAAAGAACTTGAAACAATATGTATGCGATTCGAAGGTGTTAAAAGCGCCTATGTCATTAAATCTGGTCGTCAATTACGTGTAATTGTTGATTCTAACTTGGTAAGTGATGAGCAATTAGAACTCTTAGGTCACGAGATCAAAGTTGCAATTGAAGAAAATGATTTATTAACAAATTATAAAATTAAGATCGTTTTAATTAAAGAAAAAAGGATTTCAATTGACACAAATATTATTGGATAGTATGAAATAATAAAAAAGAAGCAAGACTACCTTGTTTCTTTTTTATTATTTTCTTTTATATGTTTTTTATCTGAATTTTTATTTAGATATTTTTGTTTTGTTTGCTCGTCGTTGCGTAATGCTTTTGAAATTCCTCAAATACCAAACGACAAACTAATAGCAAAAATAATACCAACGATCAATCAAATTTTTGATTTATTATTTGTCTTGCTCATTTTCTAACTTTTGTAATATTTTTGTAACTTCATCAACGTTTTGTTCAACAGAATTGTTTGCATCTAAAACAACATAGTTAATACCAAAATCTTTACAAACATTAACCATATAATTTTTGTACATTTCTAATAAACGTTTGAAGTATGTTTCATTAATTGAAAAATTATCAATTTCAACTTTCCGGTTTCTTTTAAAAATTCTTTCTTTGAATAACTCTCAATCACCATCTAAAATGACGTAAAGTTTTGGAACTCCATGTTCATAGATTAATTCTTTGCAAACTCCTTGTCACAATCCATCATAATATGAAAATACAGAAGGTTTATCAATATTTGCATGCGCAAATAATCAATCTTCAAAAATACTACGATCAAAAATTGTTAAACTTTTACGGTTACAATTATTTTTGTAGTTTGCAAAACGATTTAAAACAAAATATAGTTGAAATAAAGACCCAAAAAGAACGTTATTCTCACGTTCATACATTTTTGCTAGTAATAAATCCATTAATTTATCACCCTCAACCATTTCATAAACCACATTTGATGGTTGATATTTTTCGTGTAAAGCTTGTGCTAAAGTAGATTTACCAAAAGCAATCATACCACCTATTGCAATAGAATTAGAAACCCTATTTTTTTTAAAAGATGGTGTTAAAAAATCTAATTTTTTATCTGACATATTTTAACCCTTATCATAAATATTTTTTTAGTTCATATTTTAGATTATACAAATAAAAAAACTAAAAAATAAAATTAAAAAAATAATTTTTTTATTATTAAAAAATGTTAATTTTATGGTATTTATAATAGATAAAAATTAAATTTTGTGCTAATTCTAATTTATAATTAAAACTAGTAGTTTTTAGGGGATAATAAATGAGCGAATCAACATTGTACCGTAAATATCGGCCACATAAATTTAAAAATGTAATAGGTCAAGATTTAATTAAACAAGCATTAATGAATGCTTGTTTAAACAACAAAATTACACATGCTTATATTTTTAGTGGACCACGAGGAATTGGAAAAACATCAATAGCTAGAATTTTTGCGTCTAGTATTAATTGTTTAAATCCTAATAAAGGTGATTGTTGCGGCAAATGTAGTGTTTGTGAAAGTATTCTCAAAGAACAAAGTGTTGATTTGATTGAATTAGATGCTGCTAGTAATAATGGTGTTGATCAAATACGTATTATTACTGATAATATAAATTATTTACCAACATTTTTTAAGTATAAAGTCTATATTATTGATGAAGCACATATGCTATCTACATCAGCATGAAATGCTTTATTAAAAACAGTTGAAGAACCACCACAACACGTTATTTTTATTTTTGCAACAACAGAGTACCAAAAAATTCCCTTAACAATTATTAGTCGTTGCCAACGTTATGATTTTAAGCGTTTAAATAACAGTGAATTACAAGAATTAATAGACTCTATCCTTACAAAAGAAGATATCAAAATTGATAATAATGCAATTGATAAATTAATTCAATTAGCTGATGGATCTGGGCGTGATTGTTTAAGCATTTTAGATCAATTAACAACGGTTAAAAAAAATATAGATCTTGATTTAATAAATAAAACTTTTGGTTTAGTTGACAACTTTAAAGTAATTTCTTTAATCGAATTAATTCAAAAAAACGACATTTTATTATTGCGTGAATTTATTTATGAATTATACAATCAAGGTGTTAATTTAGAAGCTTTTTGTGTGCAAATAATAAATGTTTTAATTGATTATTTAATTTATTTAAAAACAAACGATATAAATAATCTTAAAAAAGTATCAGTTGATGAATTAAAAAAAATGTTATTAATTAATTTTAATGGTAATTATTTGTTAAATAATTTTATTTCTTTATTTAATAATTTAAAAAAATCATTAAATCAAGTTTTTGAATTTGAAATTTATTTATATAAAATAATCAATGCTAATAATGATAAATTAGAAAATAAAAAAATAGAATTGTCAATTAACCCTGAAGAAAATAAAATTAATAATTCCCAAATAATAACGACAAAAAAAATCGATTGTTTTGAAGAAATAAATAAAAATACTGTTGATTTTGATAAGTTATACAAAACAAAAATTTTTTATCACAAAAAAATTAACAACCAGAATGATAAAGAATCAGGTATTAATCAAAAAGAAGAATTAAGTACAGAATTAGTTAATGATAATAAATTGATTTGCAATTCTAATGATATATTAGATAATTATGAACTAGCCAAACAAGCTTTTTTTAATAAAGACATTAAACTTTCAAAAGAAATGAGTCAAAAATTTAAAGATTTTAAAAATGAAGAAACCATTGAAAATAGTTATATTGATATTATTAAACAAGCTGATTTAGTACTTTGGTGCTCACCTAATGCACTAGTTTTAGGAGTAGAGTTTTTAGGATTAATTAATCGTATCAATAAAGTTACGCATTCATTTGAATTTATTAAAGAATTTGTTAAGAAATTTGATTCTATCAAGTTAGTTATTGCTATTAGCAAAAAACAAGCATTAAATATTATTAATATTAAAAAACAAGGTTTAAAAACTAATATAATTAATGATGTTTTAATTGATGATATTAAAATGTTATTAAAACAAGAAGAACAACGAAAACAAGAACAAACAGCGTTATTAAATGAAATTGAAGATGAGGAGTAAAATTTAAATGGACTTTCAAAAACTTGCACAAGAATTAAAAAAAATGCAAAACACTTTAAGTAAAAAACAAAAAGAATTTGAAGAAAAAGTGTTTGATTTTGATTATAAAGGATATGTACTTGTTAAAATCAAAGGTGATTTAAATATTGAAGCAATTGAAATAAAAACAGAAATAGTGGATCCTGAAGACAAAGAAACATTACAAGATATTTTACGTGCAGCGATCAATGAAGCAATCTCTATAACATGTAAAGAACGAGATGCGATTATGAATGCTACAATCCCTAAAGGAACAGGTCTTTTTTAGAATTAATTATGAGTAAACCAGTAGAATTTGAAATGCTTGTTGATGCACTTAAATCATTACCTGGTGTAGGAACAAAAAATGCTAATAAATGAGCATTTTTTTTGTTACAACAAGATCAAAAATACATTGATGATCTTATTAAAAGAATTAAAGAAGCAAAAACAAATATTTTAAAATGTAAGTATTGTGCTAATTTTACAAATAAAGACGAATGCGATATTTGTTTAAATGAATATCGTGATTTTACTAAATTAATGATTGTTACCACAAACGAGGATTTAGAAAGAATCGAATCAGCTAACATTTATAATGGCCTATATCATATTACAAATGGTGAAATCTCTTTAAGGAAAAACGTTGTTATTGAACATACAAACATTAAGACTATTAAAGAGCGTGTTTTAAATGGTAGTTTTAAAGAAATAATTATTGCTACAAGTTATACCCATGATGGGGAAGTTACGGCTGATTATATTATAAGAATGCTTGAAGACATTAAAGATTTACAAATTTATCGTATTGGCTTTGGTATTCCTTTAAATTCAAGCATTGATTATGCAGATGATGAAACTTTAAAGCAGTCTCTTATTAATAAACGGAAAATAAGAAATTAATTTTTTATTTTAAAACAATCTTTAATTATTCATTTTAAATGGGGGTTATAATAATTGTATAATTATAAGGAGGTTAAACATATGGGTTACGGTAAAGGATACTTAGCAATGTTTAAAAACAAGAAAGTAAGATTTAAGGTTGTAAATAGCTTTCCTGATCTAAAGGTTCAATTTGTTACATCATTTCCTGATTACAAAGTAAAGATTTCAAATTCATCTTCTTTTTGTGAGGAAACTATTAAAATTCAAGTTGTTACTAGCTTTCCTGATGTAAAATTACAAAAAGTTACATCATTCGGTGATTTTGAAGCTTATATTGACTAAGAACTAGATAATAAAAAATTTTTATTTTTTGATTGAGCTGGTGGTATGCTGGCTCATTTTTTTAATATATTTAATTTGAGAAGAGGCGAGTTAATTATGGCTAATGAGTATTATAATATGTGTGCCTTAGATGCACTCAAATTTGTGAAAAATAGAAATATGTTATTACCAGATATTCAAAGAGAATATGTTTGGGATACAACTGATATAGAAAAATTATTTGAATCTATAGTTGATGATTATCCAGTGGGTTCTTGTATATTTTGGAAAACAAATAAAAAAGTACTTAATGATGAAAAACCAAATCTTTATTATTTTATTTCTAAATATAAGCAAGGCGTAACTAAAAATGAAAAAGCACCAGAATTTGTATCTGATGAAACAGATTATTATATAGTATTAGATGGACAACAAAGAATTACATCTCTTAATATTGCATTGTTTGGATCGTTTACATCTAAGAAAAAAGGTAAAGGATATAGTGTAAAAAATTCTAAATCATGGGTAGAACGAGAACTATATTATAATTTATCTTTTTACGACAGCAATGAATTGGATGATGAAAATCCCATTAAAAGATTTGTATTTTTGTCTGAAGAAGAAGCAAAGAATGGTAATTATTATAAAGTTAAGCAAATAGTTAAAAGTGATAACTTAACTGATTATGTTGATACAATATCGAATTTTAATAAAAATGTAAAAACGGATTTAATGTGTTTGTATAGAAGAATTATGGAAGCGTCTGCCACTAGTGTAATTCATTATTATTGCATTGCAGAGAATACATATGATGAAGCACTAGATATTTTTGTTAGAGTAAATTCTACTGGAAGAAAACTATCAAAGTCCGATTTATTATTTTCTACATTAATTGATGGTTGGAAAGAAGGGAAGGAAAATATAGACACCTTATTGTCTGACTTAAATTCAGCTGGAGATGGATTTTCTTTTACAAGAGATTATTTAATGCGATTGGCTTTGGTATTAGTTGATGCAAATACTAATTTAAAAATTGATTCGTTAACTTCTAAAACAATAAAGGAAATCAGGGATAACTGGCAAAAAATAAAGAAAGCGCTAGAAAAATTATCTGAATTATTAGTTTCAATTGGCATGTGTGACGAAAATATGACCTCATATAATGCAACCATTCCACTGGCATATTATTTTTACAAAGGTGGCAAAATTGATACAGCAGAATCAAAAAAAGAAGCAAGAAAGTTCCTAGCCGTTTCAATGTCAAAAGGACTGTTTGGTGTAGCAAGTAATGATTCTTTGAATCAAAGCAGAACTGCTCTTAAGTCAGTAAATGAAAAAACTCCATTTTCATTAACTTTATTTAGTAGTATTACTTTAACGGGTAATAGAACATTTACTGCATCGAAAGATGATATTGAATATTGGTTGACGAAGTTTGAAAAAGGAAAAAACACATTCATAATTCTTTCTTTGCTTTATCCTAATTTAAAGTTAAGCCAAGTTGAGTTCCATCAGGATCACTGTCACCCTTATACTTCATTTGATGATAAAAACATTAAAAAATTAGGATTGACAGAAGATAAAATAAAAGAATGGCAGTTTAAAAGAAATTTAATTCCTAATTTGCAGTTTTTGGCAGGTAAGGAAAATGAGTCAAAAAATAAAAGGCCTTTATCAAATTGGGTTAAAGATGGAAATACCTTTGAATATAGACCAGAAAATGTTTCTCTTGAATTATCCAATTTTGATAAATTTTTTAATGAAAGAAAATCCCTTATAAAAAATAAGCTTTTGGATATTTTTGGTTTAAAAGCAAACTAAAAATAAATAAATGTATAAAAATCTAGATTTTACTCAGTTCTAAATAAATTAATTTCTGTATGAAGTGGAATTACATGGTTTTTTTGCTTGAATATTGTAAATATTCGTATGCTTTGATATAGTATTAATGCAAGCCAATGTGTATAGTGCTTTGCAGTACCTATGACCGATTAATGGTGGAGTGGTGCAAAAAAAATTATTTTTCTCTGATCTCAAAGGGTTGATGAAAGGAGGAACTTATTATGTCATCAAACAAAGTAGAAAGATTCGACTTGTTTAAGAACATAACATTATCATTTTGATAAATTAATATTTTGTTTAAGAATAGATAACATTTTAAATTCTTAGTTCTAATTCGTTAATCTTTTTCTAATTTAAGAATTTTTATTTATAGATTTGTTTTTTTTAACTCATTGGTTATTTTAATTAATTCATTAATACTATAAATTAGAAACATTTTATTTTGATTAGAAGCATTTTGAATTTAAACTTTATATCTATAATACAAATTTTGTGTTTTTTATAATTTTATAAATTTCTTATTAAGAAATACAAAGATTAAATAATATAATTAATGATTAGTACAATAATTTTTAAAATATAAAGAGGTAATTGATGTTCAAAGCAATGATAGGTAGTATTGTAAGTAAACAAATGTCAAAAAAATTAAAAAATGCGACAATTGTTGAAGAAGATATTAAAGAATTATTAAGCGAGATAAGAATAGCATTATTAGATGCTGACGTTAATTTATTAGTTGTTAAAAAATTTATTAAAAACATCAAAGAAAAAACAATTGGATTGTATGTTGAAGCAAACCAAAAGCCAGCAGATCTTGTTTTAAAAATAATTAAAGATGAATTAGTTGAAATTTTAGGAAAAGAAAATAAGCCAGTTAACACAGCTAAATCACAATTAAAAATTATGATGGTTGGTTTACAAGGTTCAGGTAAAACAACAACTACTGGTAAATTAGCTAATTATTTTAAAAATAAATACAATAAAAAACCATTATTAGTGGCGGCTGATATCTATCGTCCAGCGGCGATTGATCAATTAAGAACATTAGCAAAACAAGTTCGTGTAGATTTTTGAGAAGAAGGAACACAACGCCCTGATTTAATTGTAAAAAATGCTTTAAACAAAGCTGATGAAAATGAAAATAATTTGATAATTGTTGATACAGCTGGACGTTTACAAACAAACGAAGAGTTAATGCAAGAATTAGTAAATGTTAAAAAAACACTAAATCCTGATGAGGTTTTTTTAGTTGTTGATGCTATGGCAGGCCAAGACATTATTAACGTTGCTACAGAATTCAATAATTGATTAAAATTAACAGGTATTATTGTTACTAAATTAGATTCAGATGCACGTGCTGGAGCTGTTTTATCATTAACATCGTTATTAAATATACCAATTAAATTCACAGGTACTGGTGAAAAAATAGGATCAATTGATAGTTTCTATCCAGAACGAATGGCTGATCGAATTTTAGGTCTTGGTGATATCATGACTTTAGCAGAGAAAGCAGCTGATGTTATTGATGAAAAACAAGTTCGTGGTTCGATGCAACGAATGATGGCAGGAAAAATGGATTTAGAAGATTTAATGCGTCAAATGAGTCAAATTAGTAAATTAGGTAGTTTTTCAGGAATAGCTAAGATGATTCCTGGATTGAACAATATTTCTGAAAACCAAATTGGTGATGCTGAGAGCAAAATGAAAATATGAACAATTTTATTATCAAGTATGACTTTAAAAGAACGTCGTGATCCGCGCGTTTTTAAAAAAGAACCATCAAGACGCATACGGGTTTTAAAAGGATCAGGACGTTCGCCTGATGAATTAAATAAGTTATTAAAACAGTGGGAAGTGTCACGTGATAAAATGGCAGAACTTGGTAAAATGTTACAAAAAGGAAAAAATCCTTTTAGTAAATCTGGTGGGTTATTTAGTTAAAAATGGTTATACACCATTTTTTTATTTTATTTAATATAATAAATTGTTGAATAAGAAAAAATAAAACACTATCTTAATAATTTTTCTTATTATTCCATATATTTTTAAAAACAAAATAATCAAAATAAAAAAATACAATAAAACCAAAATTAAATTTATCTTTTGAATAAAATTATTAACAATATTTAATATATGGAAAAATCACATAAAAATGAATAAAAAAATAAATTTGAACAGCAGTTTAGCTTATATTGGTTTTTTAACACGCCTTATTATTGTAAAAAAAACAACATATCTTTTACCGATCATAACTTTATTAATTACATTAATTATAGGAATTGTTGTCGGCTGTGTTGTTAAGCAAAAAACACAATTTTTGATAATTTCATATGTAATGATCATGTTTAATTTATTAATGACAACAATTTTTGCTTCACTAAAAGCATTAAATATTTTTAAGGATTTTAGTGAAGATGGTATTGATATTTTAGTAATTTCTAAACCAATTAGCCGTAAAAACATTGTTTGATCAAAAATTTTTTTCTTCGTCCTAACAGGTATTGTTTGAAGTCTAGTTAGTTTTTTAGGACTATTAATTTTTTATTTAATTAGCTTTAATTTTGCTAAAAATGTTAATTATTATTGAATCTTAAGTTATATAAGTCCTTTTATTTGTTATTTAATTTTTGGGTTAATAACAAGCCTTTTAGCTTTAAAATTAAATGCAAAAATAAGTATTTTAATTCCTTTTGTTAGTTTTATTCCCTTACTTACTATTGGAATTATTGCGAACTTAGTTAGTCAAGTCCAATCACAAGCCTTTTTAAGATTATTAAAACAAAAATCAACAAACAATATTGAGGCTTTTTATTTAAATAATAATTTAGATCAATATTATTTAATTAACACAGGTTTTTTTAATCATAAATTTAATGAAAATCAAAATTTAGAAATTATGAACGCTTATTTAAAAACTAGAAGTTTAGCAACTTTTTGACAAGTAAGTAGTTGAATTCTTCCTGTTTATCAATTAGCTGATGTTTTTAATAAAAGTGATTATGAGCCATTTAGTGATTTTATTAAAAATCAAGTAGATCAGATTTTATATGCCAATAATTTAGCATCAAAACAATTTAATTATCAGTTAGAAAAAAATAGTAATTCATTAATCAATTTTAGTATTAATAATGATAAAAGTTTTTTAATTCCAAGTTTGTTAAAAAACGATTCTCAAAATTTAATTAACAATAACTTAAATCAAGAAGTAATTTATGCAATTGACAATTGAAAAGATCAAACGATCGAATATCAAAAAAACAGTTATACACAATTAAGTAGTGATGATATTGTTGGTTCGCTTAAATGAATGATTATTAAAGAGGTATTAAACGTAAGATCGTTTAATACATATGCTAATAATTTATTTAAAACACTTGATCAAAAAGCAAATCAAAAACAAATCTTAGATTTAATTTCAAATAGTGTACAAAAATTTGATTTCAATAAATGAAACGATGAGAATGCTGATTTATTTAAAAAGGATTTTAATAAATTATTAATTAAATCAAAAACTGAACAACAAATATATTTAGCAGTTAGTTTAATTTATTATCTATATTTCAATCCACACTATACGAATTTACTAAAAATTTTATTAGTTGATCATCAAAATAATTTACAATATAAAACACAATTAAAAATAAATTTAAATAATAAAAATTATTTAATTGGCGGTTTTAAAAGCTACTTACTTCATCAGATATTGCCTCAAGAAAATGACGATGGCATAGATGAATTAAAAAAAGATAAAAAGATTCGTTACAGATATAGTTTAGAACAAGGCGGAAATTATTTATTCGATTATGTCCAACAAAATTATAGTTTTAAAAGAAAGCAACAAATTGTTTATAAAGAAGCTTACTGCGTAATTTGATTAATATTAATTTTTAGTTTATTAATTGGTACATATTATGGCTATAAAAGAAAGGACTATCGTTAATGAATAATGAATTTATTTTAGAAGTTAAAAATTTAACAAAAATTTATAAAAAATCAAACCAAGGGGTTTTTGATTTAAGTTTTAATGTAAAACGTGGCGAAATTCATGCATTTATTGGTGAAAATGGATCAGGTAAAACAACAACTATTAAATCAATTATTAATGCATACACAAATTACAAAGGATCAATTTTAATTGATGGTTTTGATAATAAAACCCCAAAAAGTCATGAAAAAATTGGTTATGTGCCAGAAATTGCTTTATTTCCTAGTGAATTAACAACTTTTCAATATTTATATTCATTTGCTCGTTTATCAAATTTAAATAAAAAACAAGCTATTGAACGCATTCATAATTTTTTAGAAAAATTTAGAATTAGTGATTTAAAAAATAAAAAACCAATCAATTTTTCATCTGGACAAAAAAAGAAAGTGATTTTAATTCAAGCTTTATTACACGATCCAGAGTTAATTATTTTAGATGAACCAACAGCGAACTTAGACCCATCAGCACGTCATGAATTCAATAATATCTTAAAAGAATTGCACGAACAAAACAAAACCATTTTTATTTGTTCGCATATTTTAAAAGAAATGGATACTTACGTTGATAGTTTGACTTTAATTCACAAAGGAAAGCTAGTATATAGTGGTCATAAATATGATGCATTAGAGAAAATCTACTATGACAGTGTTTTAAAAAATAAATAAGAATATCTTTCATTCTTTAAAAAAAGAACGAAAATTTTAATTATTTAATATTAATAGATATTTAAATTCTTTATAATTATACGAATACAATTAAATTAATTCAAATTAAGGAATTAATACTATGTTTAAAAAAAGAAGTTCAAGTCGTAAATGATTTATAATCTGTACTTCATTATCAATAAGTAGTATTGCTGTAGTTTCATTAAGTGCATGTGCTAACACAAATAATAAAATAGTAAACGCAGGTTTTATTTATAATGGATCAACAACCAACGACGCAATTACTGGTTTTGATGTAAACGATTACATAATTAAAGCGCCAGAAAATGATACTAATTTAAATCAACAAATTTTGAGAAATAATAATTATGAACAAAAACCAATTCTTTGAAATGATTATTTAAAAGAATATGATAGTGTTAAAAAACAACACGATCAACAAATCAAGAATTTTTATGAATATATTTGAATTAATGAAGCATCAAAATATAAAAATTTAAATAAAGTTATTGATATTAATGACCCCTATTTTATAAATTGATTTTTAAAATTAACGCCTAATACATTAAAAAATGATCTTTATAATTTTATTAATAATATTCGTAAAGACAACACTAAACCATCACTTGTTTTTTCATCAAGTGAACCACAAATTAAAGTTTGAAAAGATTTAGGAAATAATCAAAAACAAGTTTTAAATCCCAATCAAGCCATTTTTGAACCACTAACAGAACAAGATATTAAAAATAATATTAATCTTTTAGAAAAGAATAAGGCGTTAAGAGTTAGCATTAGTTTTTGGTATGCTTTAACAAATCAAAGTAGTGCTAATATTTTAATCAATGATCCATTTTATAAAAAACCTTCTGGGATTGCTTCAAATACTAGTGAAAAATATTTTATTAGGATTGAAGCATCACCGATCGCTTTAGATTTTAGCCAAACCGATCGATTAGAACGCGATAAATATAGTGCACAAACCACATCAATTAAAAACACATATAAAATGCGTTATTATTTTAATAATATTCGTGTTGAAAAACAAGTTACAAAGGTTAAAAACAATGCTAAACGTGGAGACCAAAACGCAGAAGAAAATTTTCCAAAAGAATTTTATTTCGGTGCTAATAGTAATGAAAAATTTAGCTTTGGTACACACTCATACACATTAAATAATGAAATCACACAAGCTTCATATGAACAAGATATTTTTAGTTTAGATCATAAAATTAAAAACGAATTAAAAAAATTAAATGAACAACAAATTTTAAAAGATATTGAATATGCATTTACTAGTGGTTATGATGTAGCAATTGATTCCATTGGTAGTATTTTTAACATTTTAAAAGGTGTTGCTAATGATTTAAATTTAAAAGAATTGTTTTTACAATCATCACAAGATTTTAGAAATTTAACTTATAACATTACACAACAGAATAATTTGACTAATTTAATCACTTTAATTACATCAAATCAATCATTAGGGATGGTTATTGATGGTTTTAAACCAATTTTAAAAGAAATTATTTATAATAATCCAAGTATTGACCAATCAGTTAAAAATACAATTTGATCAAAAATTGATTCAATGGATTTTAAAAATAATTTAATTGCTGAAATTGGACAAATTAGAACCTTACTTGATAGTATCTCATTAACTGAAATTAAAACCTATAAACCAATTATTAATAAATTATTAGAATTAATTTCTTTAATTGAAGCACGTTCTAAAAAAGACCCTAAAAATTATGGATTTATTGATGGTTTGGACGAACTATTTTCCTTCTTTTTAAATTTAAAACAAAATGATCTACCAAGTAGTATTAATTTTAAAATTGGTGATCAAAATTATACTTTATATGATTTAATTACTGAAGTTAAAAAAATTGCAAATAACTTAATTCCGCATGAAAACCAATATGACCCTAAAGACCAACAAATTATCAGTACCAATTATTTTTTAACAAAAATTAAAATTTTAGATTTGATTTCTTTAAACAAAAATGACCAACTTGATTATCATAAGGGTGTTGAAATGATTTTTAGTTTATTAAAAAAATTTCAAATAGCAATTCCCGAATTTATTTATAAAATTATTGATGAATTACTAATTCAAAATACTAATTGAAATAAAGAAAATATTAAAAAACTTTTAGATGCTATTTTATATCCAAAAATTACATCTAATGATCCTTCAATTAATGATTTAAAATCATATTTTAAATATGGGATTAGCAAGCCTGAAATCACAAGTAAAGAACTTGAATATGATCAAAACAATTTATTAATTAAGAAACTAAATATTAAATATCGTTATAAAGTTTTAGCTAATGCAGAATTTGATATTAAACCTTTATTTGATTTATTACCACAAAAACCATTAAGTTTTATTGATCTTGGTGGATTATGAGAACAAATCAAAAAAGAATTTCCTTATAAAATTGTTTTAGCAAAAAACGATTATGTTGATCATACAATTAGCATTAGTCAACCACAAGAGTTAACAACTTTAATTTTTAAAGACCGTAGTGATCATGATAAATATAAAATTGGTTATGTTTTTTATCCAACACACACAGTTCAAACCCATATGCCTAATTCTATGAAAGCAATCATTGAAAATATATCATTAAGAAACGATCGTTTATTACCTAATAAATATGTTGCTCAATTATTAAATTGGTTATTTTATAAAAAATGAGTATTCATAAATCCACTAGCGATTTATGAAACTTTTGAAGGTGATAAAAAGATTCCTAATGACAAAAATGTTAAATATTTAATCAAAGATAATTTAAACAAGTATTTAAAAAACTATGATTCAAATACATATTGTGAAGGCTTTGATTTCAAACATTTTTCTAATAATTTAGAAAATAAAGTTGGTAATCAAACAATTCGTAACTTAATTTTAGCTAAAATTAAAACTATTAAAACAGATGGTCAAGAATTATATCAAGACGCATTAGGACGAAAAATCATTGCAACTAGTGCTTATGATCAAATTAATTTAACGCTTCAAGAACTAATTGATTATAAATTGCTTGGATTTGGGAAAAAAGTTGATTTAAAAAAAGATGTTTATTTGAGTGCAGTTGCATTTAATTTTGGTTTAAACACTAACGATAGTGATCAAACAAACGTCTTAAATAATAATGATGAAATTAAATACAATCTAAATATTTTAAAAAAAGTAGTTATCACATTACACTTTACTAAGCCAATGTTAGATTTAAGTGATCCTAATCGCACTAAATTAGTTAATTCTTATACTTTTGTTGTTTAGTTTTGTTCATACATAAATTGATTAATATGAATGTTTTTTGGTGTAATTATAAGAGCATCATTAATCGTCATAATTTCTTTAGTCTTAATTCTGCAAACTATTGATAATAAAATTTTGTATTCAAAATCGTTTGAATCATTGTTATTTTACTTAACTACTTTTTGAATTTTTTTCATCACAATATCTTCTTTTGAAAAATTACTTGGTAATTGAATATTTTCTTTTCCAATATAATTTTTATATAAATCTATTAGCATTTGGAAATTAATGATCATTTTCAAAATTTTCTGCCTTAGAAATCGATTTGTTAAAGCTAAATTCTATTAAATTTTTTATAAAAGGAATTAAAGCAAATATATAATATTAGTTTTCTTTATCTTTCTTTTTTATACAACAAATTGCGATTCTTTGCAAACATCAAATCCCAATGATATTGTTCTATAAAAATCAAAATTATGAGTTAAAACTATTAAATTTGAAATTTTTATTGCATATATATTTTTATAATCAAAAGAATCAGCAATATCATTAATCACAATAAGAGTTTTCTTATTATTTTTTCAATTTTTCTTTATTAAATATTACATTTAATAAATATAAAGACCTTTTTTCTCCTTGACGCAAAAGTATTGTTTTTTAATTTCGAGCGATTTAAAATTTTTAACACATTATTTTTTTGAATTGAAATTTAATTTTTGGAAAACTTTCTCCAATAAGGATCATTTTCTATAGCATCTCGTAGTGCTATACTCTTAGAACCTTCTAATGATTTTATTATTTGTTTGTAAGCTGAAATATTTTTAAATTCTGATTCAATTTCTTTTATTTTATTTTCAAGTTTTTATACTTTTAATTTCTATATTTTCATTAACAAAAAATTATTAGATTTTAAACTTTTTAATATATTTTTTAATTTTGAAAGGGAAAAACCCTTAGAAGAAATTTATGATTTTGAAAATAAATATCCTTTATCTTTTTTAAAATTGAAGAATCAAAAATATTTGTCTATTTAACTTCTGGAATATATGAATTATTTTATTATTTATTAATGATTTTAATTTTGATAATAATAAATCATCACCCTTATTAAAAAATGAATTTAATTCTTCTTTTAATTCACAAACCCTTTTTTCTAATGAAATTTTTCAATTTTTAATTTAGATTTTTCTTCTAAATCTTGTTTTAAATCTTCATGTATTAATAAATCTGAAGCATATTTTGATTCATAATAATTTTCAAAACTGTTTAATTACAAACACTTCATCACTTCTTATTTCGCGACCATCTACAGGTATTTCATACATAGGTGTTACAAAAATTTGGTCTTTTATTTCGCTCTGTTTATTTTTTTGTTTTTTTGAAAGTATTTGCTAATGAAGTTTTCATTAATCTATTTTTATCATAAATTGTAATTATATTTGATCTGGTAAAATAAATGTGTGTTCCATTTTATCTATACCAAAACAATTTTCTAATTTACATTTAATTTTATTTAAATTAATAATCTCATTTCTTTAAAAATATATGCTAAATTATATAATTAAAATAAGTACTAATTGTAACGCTATTATTGTTTCAAATATTTTTTATTTTAATGTTCATTTAACCACGGAATTTTTCAAAATATTCGAGTAATTTTTCAGTAGCATTTTCTTTTATTTTAGACCTATTACTATTCTGATTACTTTTTTTAAAAAAAGAGGTAGGAGGCATTAAATTAACAATATCTACTCCATTTTCATTAAATTCATTATTTTCAAATGCTTTTTTCATAAATTCAAATGTTTTTTTGTTATCTAATTTATTTTCATTAATTATTTTTATAAGCTCACTTTCTTTTTTATTTTTAATAAACTTACTCCATTCATTAAAAATATCAATTTCATCCTTATTAGGTAGTTTATTAATTTCATTAACAAATTCAATAATTAAATCTTTCTTTTTTCGCGCTTTTAGACTAGATAAAATATCTCTTTCTAGATCGTGATATTTAACTTCTTTTAGATCTTTTTTGAGCTTTTCATTCATTAACTTAAGAATATAATCAACACCAATATCTTGTTGACTAATTAATTCAATTTTAAAATCAAGATCACTAACAATGCTATCTGTTTCCATACGTTTTTTATCTTGATATTTATCACGCAACCCGATATATATACTTTGATAATCTTGCATTTCTGTACCAATAAGATCATCGTTTTGTTCAAATTCACTAAATTGTGAAAGCAAATTTTTATATTTTAAAATATCACCAAAAAGTTTAATAAAAATTCTTTCCTCTTCTTTTGTTCCATTAAAAGGAATTTTAAAGTTACTTTTTAATTCTTCTACTAAGTTTCGATAACCTTGTATATATTGATTATCTTCATTATAATATCCTTCATAATATTCTTTAAATGATCTTAAAATAGCGATCGAAGAATTAATATTGCTCTCTGATGAAAATAATCTTAAAGCTTCATTCACTTCATCTTCTAAATTTATGAAAAAGACAATATTACCATTTGATTTAACACCATTAAGAATTCGATTGGTTCGCGACATTGCTTGAATTAAACTATGATATTTTAGTTTTTTGTCCAATCATAATGTATTTAAAGTTGTTGCATCAAAACCTGTTAAAAACATATTAACAACGATTGCTATATCTAAGCTTCGATCCTTTAATCTCAAGCTAAAGTCTTTATAGTAATTATTAAAATTAGCATTATCTGTTGAGTATGATGTCCCAAACATTTCATTATAATCTTTAATCGCTTCATCAAGAAAATTACGTGAATCTAAATCAAGTTGATTAGTGTTTTCGTTATTCTCATCTAATAGTTCAAAATTAGAAAGTTCATTATCATTACTTTCTTGTTCGCTTGGTAAATAACTATAAATTAATCCAATTTTTAATTTTTCTTCTTGAGGAAATTTTTGTTGTTGTTTTTTAAATTCTGAATAATATATTTTAGCTGCATTAATAGAAGAGCAAGCTAGAATAGAATTAAATCCATTTAGTGTTGTTAATACTTTATTTTCGCTTAATTTATTTCTGCTAGTAATAACTTCATTTATATTAGCAATCTTATTATGTTCAAAAAATTCTTCATTTCTTTTGGTTAAACGATTATAGTTTTTTAAAATAAAATCAACAATTTCAGAAATACGAATCGGATTTTCCAAAGCTTCTTTTTTATCTATTCCAGTTGATGATGTTTCATCGTCAATGTCATCTTTTTCGCTAAGTGTTTTATAATACTCCGTTCTAAAACCTAAAACATTACCATCACGAATAGCTTTATCAATTGTATATTTATGTAAACAAGCTCCAAAAAGATGCTCTGTTGTAAAGAAAATATTTTTATTTTTGACATTACTATTTTCTTCTAAAATAGGTGTGCCTGTAAACCCAAAAATCATATATTTTTTAAAAGATTTTTTAGTAATTTTTTTATGTAAATCACCAAATTGAGAACGATGACATTCATCAAAAACAAGAACAACATTTTTAGAATAAATAGGATGTTTTGGATTTTTACTAATGAATATGCCAAGTTTTTGAATGGTTGTAATAATAATTTTATTCTCTTGATTTCCAATTTGCTTAGCTAGTTCATTAACATTTGTATTAGCACTAACACATCCTTTAGAATACTTTTCATATTCTTTTGTTGTTTGATAGTCTAAATCTTTTCGATCAACAACAAATAGAACCTTATCCACACCTTCAATTTCTTTTGCTAAAGCGGCAGTTTTAAATGATGTAAGAGTTTTTCCGCTTCCTGTTGTGTGTCAAATATAACCACCAGCGCCTGTAGTTCCTAATAATTTTTTATCATTTAAAGAAATCTTAATTCGATTAATAATTCTTTCAGTTGCAACAATTTGGTATGGTCTTAAAACCATTAACGTATTATCGCTTGTAAAAACACAATATTTTGTCAAAATATTTAAAATAGTATGTTTAGCAAAAAAGGTCTGACAAAAATCGATTAAATCAAGAATTTGATTATTTTTTGAATCAGCTCAGTATGATGTAAATTCAAAAGAATTACTTGTTTTTTTAGAGTTTGTATTATTTCTTTCTTTTATAGAAGTTTCTCTAGTTGTATTTGAATAATATTTAGTTTGTGTACCATTAGAAATAACATAAATTTGCGTGTAAAGATATAAATCTGTAAACGAATGATTTGCATATCTTTTAATTTGATTAAAAGCTTCTTTAAGATTAGTTCCTCGTCTTTTTAGTTCAATATGTACTAATGGAAGTCCATTAATCAAAATAGACACATCATATCGTGTTTTTTGTTGATTATCTAAATTGTTATTTTGATATTGATTAATAACTTGAATAGAATTATTATGAATATTATTTTTATCAATTATCTTTATATTTTTGGTGATACTATCTTTATCTTTTTTTAAAGGGTAGCTATAATCTTCATGAATTTTTTTTGATCTTGCTTCAATTCCATCATTAGGATTTAATAAATAAGTACTTAAAAAAGATTCTCATTCATTATTAGTAAATTGATAATTATTAAGTTTTTCGATTTGTTTTCTTAAATTATTTTTTAAATCATTATTATTTTTAATAGGTAAGTATTCATAACCTTGTGCAATTAATAATTTAATAAATGTTTCTTCTAATTCAGCTTCACTTTCAAATTTATTCGAATGAACTTTAATTGATTTAAATTCAGCAACAACCGTTGAATTTTCATGTTCTGCTAATAGTACCTTATTTTCCATAATATAAAAACAAACTCCTTTTAATATTTTTTAAAATCTAATAATTTATTTCGATAGTATTCATATTGTTTTTTGCGTTGTTTGATTTCAAAAGGTAATCCTACGCCAATATCTTTTGTATATGCTTCTAATTTATCTAGGATTTCAACAATTTTATTTTGGATTGAAATGTGAGGGATAGGAATTAATAATTCAATAATCTCTATCATGTTTGGATGTTTTACAGATGCACCACGATAATTTTCTTCAATAAGCATTTTATTTGCTATCAAAAAATAGTAAATAAATTTTAAATTACATTCTTTTTTGTTTTTTTGTGAAAAAATTATATTTAAACTATCTATGAAATAACCATTATAGTATTTTATTATTGGAGAACCACCAGATGGCAAAGCAATTATTTCGCCATAATTAATATTATTATCATTTTCATTATATTTTATATATCCATCATACAATCCTGTTGAAAGAAGTTTAACATTACCAAAATTACACTTTTCATATCGTTTTAATTCGTTTGCAGATATATGCTTAAATGACAATATTTCATTTTGTTTTTCTTTTGGAACTCCTTTAAATTTTTTGTCAAAATTAACTATTTCTCAAAGTTTTTTATATTTAACATTGTTTTGAACTAATTTTTCATATATATTCTTTAATGTTCAAATGTAATCCCTACTTAGCTCTCTCTCTCTCGTGCTATATTATCAAAGTCTAATAATTTATTACGATAATATTCGTATTGTTTTTTACGTTGTTCGATTTCAAGAGGAAGTCCAGTATTAATATCTTTCGTATATGTTTCCAATTTATCTAAAATCTCAACAATTTTATTTTGGATTGAAATGGGCGGAATAGGGATTATGATTTCTTGCATAACATTACTCATTAATTTTGGATTTCCTGATGCTTGATTTACATGTTTTTTAGTAATTTTTCTCAAAATAAAGCTCAAATATTTTGTGTTTAAATTAGAATTAATGAACTTGATCTAGGTAATTATCAAGATAATACAATCGATGTGTTCGGTGATGCTTATGAATACTTAATGAGTATGTATGCTGCAAATGCAGGTAAATCAGGGGGAGAATTTTTCACCCCTCAAGAAGTTAGTGAACTATTAGTAGAATTAACATTAATTGATTTTAATAATGAAAATAAAGATGTTAGAAGAAAAATTGGAAAAGTTTATGATCCATGTTGTGGTTCTGGATCATTACTTTTAAAGTATGCAAAATTAAATGAAGGAGTTAAATTTTATGGTCAAGAAATTAATCTTACTACGTATAATTTAGCAAGAATTAATATGTTTTTGCACAATATAGGTTATGATAAATTTGATATCAAATTAGGTGATACATTACTAGATCCTAAACATAATGATGATAAACCTTTTGATGCAATTGTTTCAAATCCTCCATATTCAACAAAATGGGAGGGAAAATCAAATCCTTTATTAGCTAACGATGAGCGATTTCATGTTACTCAATTAGCACCTAAAGGAAAAGCTGATTTTGCTTTTGTATTGCATATACTTCATAATTTATCTTCTAGTGGAACAGCAGCTATAGTTATGTTTCCAGGAACTTTATATCGAGATCATGCAGAACAAGATATTAGAAAATATTTAGTTGATAATGTTAATGTTGTAGATAGTGTGATTCAATTACCAGATAATCTGTTTTTTGGAACAAGTATTTCAACATGTATTATAGTTTTAAGAAAAAACAAAAACAACAATGATAATGCTAATGGTATCTTGTTTGTTGATGCATCAAAAGAATTTGTTAAATCTGGTATCAAAAACAAATTAACCAATGCTAATATTAAAAAAATAGTTGATACGATTCGTTTCAAAAAAGAAGTAACTTATTTTTCAAAACTTGTATCACGAGAAGAAGTTAAAAATAAAAACTATAATTTATCAGTTAACACATATGTGGAAAAAGAAGACACGTCAGAAAAAATTGATATTAAATTATTAAATATGCAAATTAAAGAAATTGTGGCTAAAATCGAAAAGTTAAGAAAAGAAATTGATGAAATTGTTTTAGAATTAGAAGAATAAATTATGAATAAAATTGAAAAATTAATTAATGAGTTCTGCCCTAATGGAGTGGAGTTTAAAAAATTAAAAAATATAATTACAGTGGCTCCTAAATCACCTTTTGGAGTTACAAAACTTTTAAAAATGGAAAAGGGAAATTATTTAACAATAACATCAGGGAAGAAATCATTTTATGTTGACAATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGCGGTCAAGCAGATATAAAATATAATTTTGGAAAAACAATGTATTCTGATCATATATTCGCATTCAAAGTAAATGAATATAACACAAAATATCTTTACTTCTATCTATTAAATATTTCTAATTTTATTAATAAAAAATTGTTTATAGGTAGTACATTAAAAAATTTAAATAAAAAAGAATTTTTAAATCTTGCAATCCCTATTCCGCCCATTTCAATCCAAAATAAAATTGTTGAAATATTAGACAAGTATACAGAATTAGAAACAGAATTAGAAACAGAATTAGAAACAGAATTAGAACTTAGAAATAAGCAGTATATATATTATCGCAATGAATTACTAAACTTTAATAAAAATCAAACATTATTAAAGAAAATAATGGACAGTGATGATATAAAATCTATTGATCATAAAATTGAGTTTAAAAAGATTGAATATGTGTGTGAAATAAAAAGAGGTCAAGTATACTCTAAGGAATTTATTAATTCTAATAAAGGAAATTATCCAGTATATTCATCACAAAGTTTAAATGATGGTGTTTTAGGAAATATTAATAAATATGATTTTGATGGTGAATATGTTACTTGAACTACAGATGGTGCATATGCGGGAACTGTTTTCTATAGAAAAGGCAAATTTTCAATAACAAATGTGTGTGGAATATTAAAGGTGTTTGATAATTCTAATTAACAAACTTAAAAATATAAATTTATAAAGGGTTTATAAATATGGATAATAAAAAAGAAATTGAACGAAACAATTTACATGCAACAATTTGAAAAATTGCTGATGAGCTCCGTGGCGCAATTGATGGATGAGATTTTAAACAATATGTTTTAGGAATATTATTTTATCGATATATATCAGAAAACTTAACTAAATATATTAACGATAATGAGCATAAAATAGGTGATCATGATTTTAACTATGAAACATGTAATGATGATGATGTAGATGAAGAAATGAAAATAACCTTAATTAAAGAAAAAGGTTTTTATATTAAGCCGTCATTTTTGTTTGCTAATATTGTTAAAAATGCTGAAAAAAATGAAAATTTAAATGAAACTTTGGAAACTGTTTTTAACAATATTGAAGGATCATCCTCTGGTTATGATTCTGAAAAAAATTTAAAAGGGTTATTTAATGATATTGATGTTAATAATAGTCGTTTAGGAAGTACTACACAAGAAAGAAATAAAAGACTAGCAAGAATTTTAATTAGAATTATATATAAAAATATGGATTTATTAATTTTAAAACCATATATAAATAAAAAAGAGATTCCTAATTAACTTAGTAATCTCTATCATTTAATTAAATACTTTTTAATTAATGTTGGTGACTGTGATGATGATGGTGTTCATGTTCGTGACCACCACAGCAATCATCTTTAGTTCCACCACAACATTCTTCTTGGGTTTCTGATGTTTTTGTTTCCGAGTCACAGCAAACTTCATGTTCATGTTCGTGACCACCACAACAATCATCTTTAGCTCCGCCACAGCATTCTTCTTCAACTTTTACTTCTGAATTACAACAAGCTTCATTTTCTTCATCGCTACAGCATTCATCTTCACAACAGCAATCTTCTTCATAATCATCATCTTCACTAAAATGAATATCTAAGAAAACTTCTGATAAATCTAATACTGTATCATTAGAAATTAAAAAAGCATCAATTTTTGCTTGTAACTCTTCTTTTTTAAGATCAAATAAAATGTTTGTTGATTCAAAATCAGCATAATTAATATACAACTCTGATCCTAGATCGCTTGTTTTATAAGCTTCATCTCCACCAAGTTCTAAATATGAATCAATATCTTCTGGAAGAATATTAATTGTACGTGCGTTGTCTAAATTAATGTAAGAAACACCTGTTTTAATGTATTTAGCCATAAATATACTCCTTAATTATTAAATATTATTAGGTTTCACCTTATTATTTTATACCAAATATTTCATTCTTATATTTATGATTCAAAGAAGATTATTTTTAATAAATAACCTAAGGTATTTAAATTATATATGTTTTTTATATTATATATTAAATTGTTATCATTTAAAATGATATAAATAAAATACAATTTTTATTATTAAAAACAATAACTATTAGGTATAAATATGGACAAAATTATCATTAAAGGCGCTAGAGAAAATAATTTAAAAAATATTGATCTAGAAATTCCTAAAAATAAACTAGTTGTTATTACAGGTGTTTCAGGATCAGGTAAATCATCGCTTGCTTTTGATACGATTTTTGCTGAAGGAAAACGTCGTTATTTTGAATCACTTTCAAGTTATGCCCGCCAGTTTTTAGGTGGAAATGATAAAGCTGATGTTGAAAGTATTGAAGGTTTATCACCAACCATTGCTGTTGATCAAAAATCAACAAATCAAAATCCAAGATCAATTGTTGGGACAATTACAGAAATCTATGATTATTTACGAGTTTTATTTGCTCGTGTTGGAACACCTTTTTGTCCAAATGGGCATGGTCAAATTAAATCACAAACTCCAAAACAAATTGCTGATTTCTTATTTAGCTTATCACCTAAAAGTAAAATACAAATTCTTGCACCAATTGAAATTAAAAAAGGTTATAAAATTAATGATGTTTTAAATAATTTAAGAAATCAAGGTTATTTAAGAGTTTTAGTTAATAATGAAGTTTATCAATTAGATGAAAATTTGCCTCAATTTTTAGATAACAAAAAAACAGATGTTGCAATCATTGTTGATCGTTTAATTTTAAATATTGATCACCAAACAAAAACACGAGCACTAGATGCTATTGAATTTGCTTTAAATTATAGTGGGGGAGAAATTGCATTTAAGGTTAATGATGATATTCATTACTTTACACAAAACGATGTTTGTCAAGTTTGTGGTTTTAAAATTAAAGAAATTGAACCAACATTATTTTCATTTAATTCACCAATTGGTGCTTGTGAACAGTGTAAAGGGCTAGGATATAATTATGTTCCTGATGAGCGTAAAATGATCCCTAATCCAAATTTATCAATTAACGAAGGCGGTTTAGATTATTTTAAAAACACTGTTAATACAACTAATCTTGATTGACAACGTTTTAATTCAATTATTAAACACTATAAAATTGATAAAACTAAACCTTTAAAAGAATTAGATCGTAAAGAAATAGATTTATTACTATATGGATCAGATGAAGCAATTGAGATTGATATAACTTCTGCAAATAATAAAAAGTATTCATCAATAGATTATGTTGAGGGGGTTTTAGAATTAGTTAATCGTCGTTATCAAGAGACATCAAGTGAATTAGCCCGTGAACATTACAATAAATACATGAGTGAAAAAGTTTGTAAATCATGTAAAGGCAAAAAACTATCTTCACAAGCTTTATCGGTTTTAATTAATAAAATTAACATTATTGATTTTATTGAAAAAAACATTGATGAGGCGATTGATTTTTTATTACACTTAGATTTGAATGAAGCACAAACAAAAATTGCCAATCCAATTTTAAAAGAAGTTTTAGATCGTTTAGGATTTTTAAAAAATGTTGGTTTAGAATATTTAACTTTAGCTCGTCCTGCATCAAGTTTATCAGGGGGCGAAGCACAACGGATTCGTTTAGCCACTCAAATCGGCTCTAAACTAACAGGTATTTTATATGTACTTGATGAACCATCAATTGGTTTGCATCAACGCGATAACGATAAATTAATTAATACATTAAAAGAAATGCGTGATTTAGGTAATACAGTTATAGTTGTTGAACATGATGAAGAAACAATGCTAGCAGCTGATTATTTAATAGATATTGGTCCTCAAGCTGGAGTTAATGGTGGTTATGTAATAGCAGCTGGAACACCACAAGAAGTAATGCAAAATCCTAATTCACTAACAGGACAATATTTATCAAAACAAAAAGATATTTTAGTTCCTAAAACACGAAGAAGTGGTAATGGTCATAAAGTTATTTTAAAAGGTGCAAAACATAATAATTTAAAAAATGTTGATTTAACAATTCCTTTAGGTAAATTCATTTGTGTTACTGGAGTTTCAGGATCTGGTAAATCATCACTAATTTTAGAAACATTAGTTAAAGCTATTGAATACACTAATTTTAATCCTTTTGTAATTCCAGGCGAGTATAAAGATTTAATTGGCGCATCGAATGTTGATAAAATCGTTGTCGTTAATCAAGATGCAATTGGTAGAACTACGAGATCAAATCCAGCTACTTATGTTGGGGTATTTGATGATATTAGAACAGTATTTGAAAATACAATTGAAGCTAAAGCACGTGGCTATAGTAAAAGTCGTTTTTCTTTTAATATTAAAGGAGGCCGTTGTGAACGTTGTTGGGGGGATGGTACGATTCGCATTGAGATGCACTTTTTACCAGATGTTTATATAAGTTGTGAAGAATGTCATGGAAAACGTTATAACGATGAAACACTTCAAGTTAAATTTAAAGGTAAATCAATTTATGATGTACTAAAAATGCCAATCGATGAAGCTTTAGTATTTTTTGAAAATTATCCTGGTATTCATCGTAAACTACAATTATTAGTTGATGTTGGTTTAGGATATTTAGAGTTAGGAGCCTCTTCAACTTCATTATCAGGGGGCGAAGCACAACGTATTAAGTTAGCTAAGTTTTTACAACGTAAGCCAACAGGAAAAACATTATTTGTTTTAGATGAACCAACAACAGGACTGCATATTGATGATGTAGCTAAATTAATTAAAATTTTAGATAAAATTGTTGATGGAGGAGACACTGTTTTAGTTATAGAACACAATCTTGATTTAATTAAGGTTGCAGATTATATTATTGATATTGGCCCTGAAGGTGGTAATAAAGGTGGTAAAATTATAGCTACTGGTACTCCAGAGCAATTACTAAGTAAAAAGGACATATCTTATACTGCTCAATATTTAGAAAAGTATTTAAAAAAGAACTAAATTTATGAAACATAAGACAAAAAAAATAACGCTTTTAATAGCAGGAATTACTTTAACTTCATTATGTACAATTTTTGCTATTACAGCTTGTAGTAAGCAAAAATCACAACCAAATCCAAAAATAAAACCCAATAAAATTATTGAGAAAATTGATCAAGCATTTTTTAATGAATTTAAAACAAAAATTGAACAAAAACGCACACTTCCAAATATTAAAATCACTTCAACCTTTAAAATTCCTTTTGAGAAAAACGAAAACACTTTAAAATCCCCACTTTTTAAAATTGGTGAGAAATCGGTTTTTGATTTAAAACAAAATGAAATTCCAATAACATGAGTTCAAGATTTAAAAGATTATTTTTTATCGCAAAAAACCATTTTAACTTATTATTCATTAGCAAATTTAAAACTAACAGGCAATGATGCTAAAGATGCATATTTTTTAGAATTTATTCAATATATTCAAAATATGCAACAAAAAGGAATTATATCAGAAGATTTTTATCAACAATTATGATATTTAATTAGTGAAATTAGTGACTATGATATGCAAAATGGTATTTTACGTTCAGTATATCGTATCGCTAATCCTAAACTTGCAATCAAGGATTTTTACCTTGATGGTTCGCTTTATGGATTTAAAAAAGAAAAAAGTAAATACATGAATTTTACAAGTTTGTTTCAATGACCGAATGCAGAAGAAAAAGCAACTTTTGATGTTGTTTTTAAAAATAAAAACAGCGCTCAGTCTTATACAAAAAAGATTGAACTGATTTATTCTAAAGAAAAAAAATTTATTTTAGATTCATTAATTAATTATAGTGAGTTTACTCCAGGTGAATATGAATTAGTATCAATTAAAAAACATGATGATATGAATGCCAAGAATTTGATTAATCCCAACAACCCTAATATTGCAAAAATTTTTAAAATTAAAGTTTTTAAAGAAAACGAAAAATTTAAACCAATAACCTTAACGTCAATTAATCAACAAGAAGCGGATGAATATGATCGACAAAGAAATCAAATTATTCATAAAAATAATTCAGAAATTGGGATATTAGATGATTCAAATAAAATTAAAGAGTTAGGAACTTTTTTATATTTTAAAAAACCTTTATCTGAAATTACAATCAACGATTTTGATCGTTATTTTAGAACTTATTTTATTTGTCCAGAGCAAGATTTTAATGATTTTAGTCGTCAATATAAAATTACTAAAATTGATAAACAAAAACAAATAATAGAACTAAGTATATTACATATTAATAATAAAACAAATGAAATTTATAGTTCACCAATTAATTTTAAGTTTTATTACAAAAAAATATATGATATTAACAAAAACACTGATGCACGATTAGATTTAAGTTTTGATGAATCTAAAAAAGAACAAAAACAAAAACTACAAGAGTTAGTTAATAAATTAAATTTTGATAAACTTACTTATAATGATTTAAGTGAATTAGCTGTTGTGATGTTTGAGGATGTGGATTTAGAAAACACTAAAATTTATGTTGAAATGATTAAAAATAAAGATCGTTCAATTACTTTAAAAGCTTTTGTTGATAAAGCTTTTAAAAATGGAAAATTAAAGGTAAGTAATAAACCAGATATTTTAATAAAAGAGTTTAAAATAAACTATTTTATGCCTAATTACTTTAAATAATAATAATTACTTAATGGTATAATAGTATGATTAAAAATCAAAAAACAAGAAATGATGGTATAAAAATGAAAGATATTGATCCAAGAATAAAAGAGGTTTTGATCACTGAAGAACAAATTGATCAAAAAATCACTGAAGCAGCTAATTGAATCAATAAGGAATATGAAGGTAAAGAACCAATTATAATTGGTATTTTAAAAGGATGCATTCCATTTATTGGCAAATTATTACCAAAAATTAAAATTGACATGAAACTAGATTTTTTAGCAATTAGTAGTTTTAAGGGAGCAACTAGTGCTCAAACAGAACCAGAAATTATTACTGATCTTAAATTTGAAGTTAAAGATCGTGATCTAATTTTAGTAGAAGATATTGTTGATACAGGACGCACAATTAAAAAGGTTTATGATTTACTTAAAATTCGAGGCGCTCGTTCAATTAAATTAGTTACCTTGGTTGATAAAAAAGACGGCCGATTAGTAGATTTACAAGCCGATTTTGTATGTTGTGATATTCCATTAGTCTTTATTGTTGGTTTTGGTTTAGATTATAAAGAAATTATGCGTAATTTACCATATATTGGTGTTTTAAAAGAAGAAGTTTATCAAAATGATTTAAATAATAAAAACGAAGGGGATGGAGAGTAATTAGATGTATTTTTTAAAAAAAATAGTAAATTTATTTAGTTCAAAAATTGAATCAGAAGATAATAATGTTAAAAAAGACGACTTAACTCAACCACGTAAACAATCGCCAGAAGCGCGCAAACGAAGAAATCGAAGAATTATATTTTGATTAATTATTTTATTAATTATAGGAACAATTATTGGAGTTATTATTTATTTTTCTGTTCGTAAAGAATATGATAACGTAATTGTTAAAAGTGCTCAAACAGAAGTTGTTAATAACAAAAAAGTTTTATATTTAAATACTATTAGGCCTAATAGCACTCAAATAACACGTTATACAATTGATGAAGATCAACTACTTACTGCACGTGTTAATATTTTAAATAATACTAATTTTCAAATAATTTCTAATGCATCAAGTTTAGGGCTGTCAAGAATCTCATTTAATATTGTGCGTGAAGGTGTTGAAAAGTTTAAACTTGGTGAAACAAATATCTATGCACCAATTTCTGGGAATACTAATAATCAATGAAATTGGTTAACATCTATTATTACTCAAAATGCAGGAATTCCATCTTCTGGCTTTAATCCACAAGTTATTATTTCTCCATTAATTTCAATTATTTTCTTCATAATTTTCTTATACATTATTTTACGTGTTTCTAAAGCACAAAGCGACTCTTTATTAGGAACTAATAAGGCGAATGCTAAACTAACAAAATCTGGTGTTCGCTTTAGTGATGTTGCTGGAATTGCTGAGGTTAAAGAAGAATTAATTGAAATTGTTGATTTCTTAAAAGAACCTAAAAAATATGTTGCTGCTGGTGCTAGAATCCCAAAAGGTGTAATGTTATATGGACCTCCAGGAACAGGAAAAACATTAATTGCTAAAGCTGTAGCTGGTGAAGCTAATGTTCCATTCTTTCAAACAACGGGTTCATCTTTTGAAGATACTTTTGTTGGAGTTGGTGCACGTCGTGTGAGAGAATTATTTGAAAAAGCACGTAAGAGTGCTCCCGCCATTATTTTTATTGATGAAATTGACTCAGTAGCTAAAAAACGTGGTAATTCATTAACTGCTGTACAAGACCAAACAATTAACCAATTATTATCAGAATTAGATGGTTTTGATACTTCAAGTGGTGTAATTGTTATGGCTGCGACAAATAGATTAGACACATTAGATGACGCAATTTTACGTCCTGGACGTTTTGACCGACAAATTAGTGTTAACTTACCAGATATTTTAGAACGTGAACAAATTTTAAGAATTCACTCACGTAATAAAAACTTATCTGCGAAAGTTAGTTTAGAAGATATTGCACGTCGTACTGCTGGATTTAGTGGTGCACAATTAGAAAATGTTTTAAATGAAGCCGCATTACTATCAGTTAGAGATAAAGCAACATCAATTCATATGAATCATTTAGATGAAGCGATTGATCGTGTTATTGCAGGACCTTCACGTCCTAATAAAGTTATTTCTGAACGTGAACGTGAACAAGTTTCATACCATGAAGCTGGACATGCATTAATTGGTTTATATTCACCAGGTGCTGATGTTGTTCAGAAAATTACTATTGTTGCTCGTGGTCGAGCTGCTGGTTATACACTACAAACTCCTGAAAGAAATGAAAATATTTTACAAAATAAAACTGAATTAATTAGTCGAGTGCGTACTGCATTAGGTGGTCGAGCTGCAGAAGAGTTAATTTATGGACCTAATGAAATTACAACAGGTGCAGCAAATGACTTTTATAAAATTACAAACATCGTAAGAGCAATGGTAGCTTCTTTTGGAATGACAGATGTGGGTTTAACGCAATATATTGCTACTGAAGGTGTTGATAACCCTTATCGAAATAGTTATTCAGAACAAACAGCATTAGCAATTGATATTGAAATTGAAAAAATTATTCAACGTGAATATAAAATTGTTAAAGAAATGATTAATGAACATCGTGAAGAATTGGAATTAATCGTTCAAACATTATTAGAATTAGAAACAATTTTAAAGCCACAAATTGATTATATTCACCAATACAAACAATTACCACCAGAGGTAATTGCTAATAAAAACAAACGTGAAGCTTCTCAAAAACAAGCAAATAGTAGTGTAGAAGAAGCAAAAGTTGTTGACGATGAAGAATCAATAAAAGATAAAGAAAAAGATCAAAAATCTAACTAAATTATTAAATAAAATGCTTGCTAACCTTTTTGTTTGGTTAAATAGCATTTTATTTTATTAAAATATATGTTTATATAGGTTGTTAAGCAAACTTAAAGAGGAAGAGTTATATGTTTGATATTAATTTAATACGAAAAGATATTGCAACTACTAAAGAAAAGATGTTAAATAAAAAGGTGCCAAGCAATTTGTTTGATCAAATTTTTGATTTAGATGTTTTAGTACGAAGTTTAATGCAACAAGAACAAAATCTTAATGCTAAGAAAAATCAATTATCAAAAGAAATTGGTATTTTAGCTAAAAACAAAGATCCTAAATTACAACAAACATTAGATTTAGTTAATAATATTAAAAACGAATTGCAAGATATTTCATTAACATTATCAAACAAACAAGATGAATTAAATAAATTATTATTAGTAATTCCTAATATGCCAGATGATTCAGTGCCCATTGGCAATGATGAAAATGACAACGTCGAAATTAAAAAAGTTTTTAAACCAAAAAAATTTGATTTTTTACCCCTTGCGCATTGAGATTTAGCAGTAAAAAACAAGTTAATTGATTTTGATAAATCAACAAAAATTACTGGTAGTCGTTTTATTATTTATACTAATTTTGGTGCACGTTTATATCGTGCTTTACAACAATTTTGTTTAGATATGAATGTAAAAGCAGGTTTTAGTGAAATTTGAGCACCTGTTATTGTTAATCAAGAATCTTTAATTGGTTCTGGAAACTTACCTAAATTTGCAGATGATTTATTCAAATTAGAAAATTCAAATTATTATTTATCACCAACTGCTGAAGTGCAATTAACAAATTTACATCGTAATGAAATTTTAAAAGCTAGTGATTTACCTTTATACTATACAGCTCTAACACCTTGTTTTCGTAGTGAAGCAGGATCTGCAGGACGCGATGTTAGAGGTGTAATTCGTCAACACCAATTCCATAAAGTAGAATTAGTAAAATTATGTAAACCAGAAGATAGTTTTAAAGAATTAGAATCAATGACAAGACAGGCTGAAAGTATTTTAGAAGCTTTAGAATTACCTTATAGAAGAATCGTTTTATGCACAGGCGATTTAGGTTTTAGTTCAGCTAAAACATATGATTTAGAAGTTTGATTACCATCATATAACGCTTATAAAGAAATTTCAAGTTGTTCAAATTGCACTAATTTCCAAGCGCGTCGTGCAAAAATTCGTTATAAAGAAACAATTGATGCAACAACCGAATTAGTTCATACATTAAACGGATCATCATTAGCAATTGATCGATTATGGGCTGCAATTGTTGAAAATTATCAACAAGAAGATGGTAGTATCAACATTCCTAAAGTATTAAAAAAATACATCTATTAATAAATAAAATAAGAAGTTAACTTTAGTCATAAAGCTAGCTTCTTATTTTTAATAAATACTGCTTGAAATTTTATACCTTTATTGATTGATATATCATTAATTACTATATGAAATATTTATTGGTTTTTAAATCAAAATTATTATTTTAATTTAAATAATTAAAAAATAAATTAAACTCTAAAAAAAGAGTTTTTTCTTTGCTTTTACTATTAAAATTTAAGCTATTTTTCATATAAAAAAAACTTAAAAACAAAGCAAATTTTTACTTTAAAATCAAATTTAAAAAAAATAGTTGTTTTTAATAATTTTATTAGTGTTTTTTATGTTATATTTTATTTGTTTTCAATTTTGAAATATCATGAGTTTACTCATGTTTTATTTTTTTATTTATGGAGGATATATATGGAAAAAACACTCCTACATCTTAGAGATATTACAAAAATCTATGATGATGGATTTGCTGCTGTAAACAAATTTGATTTAAAAATAAAAAAAGGTGAATTTGTCACTTTATTAGGACCAAGTGGTTGTGGTAAGACAACAATGTTAAAAATTATTGCTGGATTTGAACAACCTACTAATGGAAAAATTTTATATAATGGAATTGATATTAAGGATATGCCAATTCGACTAAGACCAACATCAACGGTGTTTCAAGACTATGCGCTATTCCCTAATATGACTGTTAAACAAAACATAAAATATGGTTTAAAACTAATGCGTAAACCAAAAGATAATGTTGATCAAAGTATTTATTTGCAAGCTGATAAAGTTTATAATTCAGCTTCTAAAAAAGCAAATGAAAAAATTAAAGAATTAAAAAAACAAAGACGCGGGTTATTGGCTGAAATCAAAAAAATGGATCTTAAATACCAAAAGAATAAGAACATTTTTGAAATAAAAGAAATGCGAAAAAACCAATATCTTGGCACACTTGATGAACTTTATCAAAAACAAGGTATTAATAAAAATGGAAAACCAGGTTTTTTAAATTATTTTAAAAGTTGATTTAGTCATGAAAAATTGAATTTAAACGATCCAATTGATAGAGAAATTTTTAATTTAAAAAAAGCATATAAAGAAAAAAGTGGATTAGATAAACGATATGATAAGATTACTTACAAATATAATGATCTTGATTATTGAGAATCATATTGAGCAACTTATCCACAATTAAAAAAAGAACAATTTGAAAATAAAAATATTACACGATTATTAACTAAAGAAGAAGTCGAAAAAGAAGCAAATCGTGTAATTAATTTAGTTGGTTTAAGTGCACGTAAGGATTCGTATCCAAGTGATTTATCGGGTGGTATGCAACAACGTGTTGCACTAGCACGTTCACTAGTAATACAGCCTGAAATTATTTTATTAGATGAACCATTAAGTGCACTAGATGCAAAAGTACGTAAACAATTACAAGATGAATTAAAAAAACTACATAAAAATTTAGGAATCACCTTTATTTTAGTAACTCATGATCAAGAAGAGGCTTTAAGTTTATCAGATAAAGTTGTGGTTATGTCAAATGGTCAAATCGAACAAGTCGGTAAACCAAGTGATATTTATGATTCACCAAATTCATTGTGAGTAGCTAATTTTATTGGTAAAACTAATATTTTTGAAGGTCATTATATTGCTAAAGGTGAAGTTGAATTTGATGGTATTACTTCTAAAACAGATGTGATTAATGGTTTTAGCGAAAATGAAGCGTGTTATATTATGATTCGCCCAGAAGACTTTGATGTTGTTAAAAAAGACGAAGGATCAATTAATGCTAGAGTTGAATCAGTATTATATAAAGGGTTAATGTGAGATATTAAGTGTAAATATAATGACATGATTATTAGTGTTGAAGGTGTTAATAAAGTTAATGAAGGTGATGAAATTGGTTTAGATTGAGATGACATTGATGTGCATGTAATTAAAAAGGATTATTTAAATAATGAACAAGCAATTTAATTTCAATTTTGCACCATCTAAATTATCAGATATTAACAAAAAAAACGAAACAGTATTTTTTTTAAAAGAAGTTAAAGTAATTAAAAAAAACAATCGTAAGCATTATAAACAAACTCTTTTTGATAAATTAAAAATGAATAAGGCACTAATATTAACAATTCCTTATTTTTTAGTAACTATTTTTTTATTGATAATACCATTGGTTAGTATTATTGTTAAAACTTTTATTAACAATACTGATTCAACTGGTGTAACAACAAACATCATTGATAATTGGTCAGTAATTACGCCAACTATTGGAATGAAAATTTTTAATTCGTTATGAATCGCTGCAGTTGCTACAATTTTTTGTTTAATTATTGGTTATCCATTTGCGTATTTTTTAAGTATTGGTAAATCAAAATTTTTAAAAGCAATTGCAATTGCACTAATTACTTCACCAATTTGGATGAGTATGTTAGTAAAACTAATTGGTATTAAAACCTTTTTTGATGTAGTAAATGGAGAAATTAATAGTACACATGGGCACATTTTTACTATTTTAGGTTTAGTTTATATTAATTTGCCATTAATGATTTTGCCTATTTATACATCTTTGTCAACAATGCCTAAAAACCTAGTTAATGCTAGTTATGATTTAGGAAGAGGTTTTTTTTACACCTTCTTTCATATTGTAATTCCTTACACTAAAAATGCTTTAATTTCAGGAGTTTGGATTGTTTATTTAGCAGCATTTACATCAGTTGTGGTTTCAGGTTTTTTAAATAATGATAACGCTGGAGGACTAATTGGTGGAGAAATCTTTGCTCAAGGACAAGACGGTATTTCAAGTGGAGTTCAACTATCACGGTCATCAACAATTACTTTAGTAATTAGTTTAATTTTATTAGGGATTTATCTTATTATTGTTATTATTCCAAAAATAGTCCAATTAATTTATTACAAACGAAAAAATCTAAAAAAAGGAGTAAAGAATAATGAAGCAGATTAATTGATTGTCTTGATTGCGTAATTTTTATATTTGAATTATTTTAGCAATCATTTATATTCCTTTAATTATCATTGTTTTATTATCATTTACCACACCTTCAATTAAAGGAAACATTACATCAGCTTTTAACTGGAATGATGGTTCAAATTATTTAACACTAACAACCAATCAATTTACAGATGCTTTAATTAATACAATTATTATTTCAGTTATCGCTGTACCAATTTCTACAATCATTGCAACAATAACTTGTTTTGGGGTTTGGCATGCAAAAATATTTTATAAAAAATTAATGACAGCATCATCACAAACAAACATAATGATTCCAGACGTAATTAGTGGTATTTCATTATCTTTATTATTTGCTGCTACTTTTATTCCAATTGGTTTTAGTTTTGGATTTGGTACGATTATATTAGCACACATTTCTTCTTGCACTCCTTATGCAATTATGATTATTTATCCTAGAATGTTAAAAATGAAAAAAAATTTAATTCTTGCTAGTTATGATTTAGGTTATACAAAAATTGCAACCTTTTTTAGAATTATTTTACCTTATTTATTACCTTCAATTATTTCAGCAACAATCATTGTTTTTGCTATGTCTTTTGATGATTTTATTATTACTAAATTGGTTGGAGGTAAAGTTAATACCATTGGAACAGAAATGTATTCGATGGCTAAAGGAATTAAAGCATGAGCGGTTTGTTTTGGTGCTTTAATGGTTTTATTAACAATTTTAATTGCTGCATTAATTAGTGCTAATAAAATTATTAAGTTAAAATTAATTAATAAACAAACTAATAAACGCAAATTAAAAATTTGAAATAGACAGGTTTAAATTATGAAAATTAATAAAAAATTATTAAAAATTTTTGCTGGAATTACTAGTTTGTTAGTTTTTATACCTATAATTACAGCTTGTAGTCAAAAATCAAAACTTGTAGTTGGTAATTTTGATTCTTATATGGATCCAGATGTTGCTAATGAGCAAGCAAAACGATTTCGTAAAAATAATTTATCATATAGTTTTTATGATAATAATGAAATTTTAAGTTCATTAATTAAAGCAAAAATTTCAGATGTACAAGTTGCTTCAGCATATGAAGTAGCTAAATTAGCAAAAAATGGATTAATTAAGAAAATTAATTGAGCAAAATTTAATTTGAAAGATGAAAATAACCAAACAATTACTTCAATTGAAGGTTTAAAAAAAATTTTCACAAAGCAAGTTTGAGCAATTTCAAATGCTTATTCATCATATTTAGGAGATTTAGATAAGGATGGTAAAAATGACCAATTATTAGAATATATGATTCCTTATTTTTACCAAGATTTAATTTTTGCTTATCGTGGTAAAAAAATTCCTAATTTGGATGATAATAAAAAAGATGTTTATTGAAGTGATGTTTTTAAAGAATTAACACGTAATGATGGCACACCCAATCGTTTTTTATATGAACAAAAAGCTAATTTAGGATTTGGTGTAGATAAAAGAACTCCGATTACAATTGCTAAAAATAAAACAAAAATTATAGCTATTGATGATGCGAGAACAATTTATGATATTGCTAAAATTATGCAATTAGAAGGGGATAATAAAAATAACATTATTAAAATTATTAATCATGAAATTCTAGGAATTGATAATAGTATTAGTCAAATTTTACATATTTTAAAAAATCCGAATATAAAAGAAGATATTAAAGAAGAAAATATTAAAAAATTAAAGCGTTTGCAAAAACAATTAGAAGATAGACAAAAAGAATTAAAAACGAATGTGTTCTTACCAGATAGTAGTAGTGTATCTTATATTGAAGATAAATTAAACAATATTAGTAATATGTTTAAAAATCTACATCCTAACTCTATGTATTTAAAAGCAAATTCAAACGATGTATTAAATGATTTAGCAATGGCTAATGTTGCTGGAGCAATTGCTTATAGTGGCGATATTGCTTTTGCTGCTAATGGGGGCGAGTATACAACTGAAGCTGATTCAAATTATGCAAACATGAAGCCAACATCAGATAATTTTCATGTTGTACGACCTAAAGATACGATGAGTGTTTTAGATGGTTTTACAATTAGTAACTCAATTAATAAACAAAACGAACAAGCAGCCTATGAATATTTAAATGAATTATGTTTTGCTGGTCTTAATGAAAAAAACACAGATGGAGAATCTAAAATTTTAGACTATGGTTTGAAAATTCATCGTAATAAAACACCAAAAGAAATTGCTGATTACGAAAATAGTTTAGATGATCAAAATGAAGTTGGTGACGTTTTAGAAGATAGTGGATATTTGTATACGCCAATGCGTAATTTTGATTATGTTCAATATTCGCCAACACTAAATACAATTGCTGATTATGTTTTAGATGAAAACAATGGATTTTATGGAAAAACTGGTTTTGAAAGTGATTTATTAAGACAAAAAATGGTTGAAATCTTTGAAATTGATACAAAATATAAAACAAAAAAAGAGCGCCAACGTAAACATAAATATATTTCATTATTAAATGAAATTATTAATCACATTTATAATACTTATCAAAAAGATAAAAAATTTATTTTTAATCCTACGTTAATTAATGATATATACGAACAAAAACCGATTAAAAATTTAATATATGAGTGTTTAAATAATTTAAATATTAAAATTGAATCACATGTTTTTAATAATTATCTTTTATTAAAATTAGAAGCTATTTTTAATAACATCAATGAAGAGGTTGAAGAACACGCTGCTTTAAACGCGACATTAAGAAAAATTTTTAATGTTCGTTTAAATATTAATACTTTAGAATTTCCCACAAATGATTTATCAATTTCTAATTTAAAATTAGCATACCTTGCCTTCCGTGAAAAGATTTAAAAATATTAATTTAAGGTTGTTAAAAGGTTCTCATTTTTCAATATAAACGTTAGTTTATTATATAATTAATATATAAGAATTTTATAATGGGGTAGTTATGAATTATAAATATAGTGCTGAAGAAAAACAAATTATTTATAACTATATTTTAAGAGAATATGGGCAAGTGGACCACATTATATTTTTATCAAATGAACATATACGTGTTCCGATTGAATATGATATTTTAGTTATTAAAAAACAAAACTTACAAATATTAATGACTTTTGGACTAGGTGCTTTTAAATCACATAATCACGAAGAAAATACGCAAGAGCGTGCTGAGATTTTTTTAGAATTACCTGTTGATTGAGATTTTAATAAACATGAAAACATGTGACCAGTTCATTTTTTAATTAATATCGTTAAATATTCTTATTCAAATCATTTAACACTAAAATGATTACAAACCTTCATTAATCCCTCTTATTTTAATAAATCAAATAAAATTGCTGGGTTTTTAGATTTATCTTGATATGGCGAGAATTCTTTAGAGTGTAAAATTAATAATGATTTTTTTGTAAGTTTTTATCAAATTTTAATTATTGATGATGAAGAATTATTCTATGCAAAAACAAATGGAATTCGTGCTTTAAGTAAATTCTTTGATGATGGTAAATCAAGAATTGTTGATTTAAATCGAAAATCATTTGTTAAATAAAATACCAAGTCTAAAACTTGGTATTTTTCACAGATAAGTTAATAATAGTAAAAGAGATAGTGATGAAAATATGTGATAATATTAGTGAAATAAATGATGATCAGGATTTAGTCTTTATTGATATTGAAGCAACAGATAGTAAGGGCGAACAACGAATTATTCAGTTTAGCGGATACCGTTTAAATATTAAAAAAAATAAAATTTTTAATTTTAATAAAAAATTCAATCCTGAACAAGAAATTAATTCACGTATTAAAACCTTGCTGAACTTTAATAAAAATTTTAATAATATTGAACAAATGCCAAAATTAGATAAGCAAGCAGCAAGAGAAATTTATTGTTTTGTTAAAAATGCGATTGTCATTACTTTCACAGATTTTGATATTAAAAAAATGCACGAGCTTTTTACATATTATAATTTCGATTTTGAAAAAATCGTTTATTTTGATGTTTATAAGTTTTTTGAAAAAAAATTACAAACTAAATCAGTTCCTAGTTTATTTTCATTAGGAATTCTAAGTGGAATTAAAATTAATTTTTTCAAATTACATAATGCGCTATATGATGCTTTTATTTTAAAAGAAATTTTTATGTGTATTCGTCATAAAACGAACGAAGAATTATATGAAATGTATCGTTATTATGAATTTTTGCCAAAAATTATTTCTTCTTCATATTTTGTTACTAATGAACAAGAAAAAAACAGGGGAATTATTAAAAAAGAAATTAAGTATGTAATGTATATTAAAGAATTTGATTTCTCAAATAAATTTAATTTAGACTTTGTTGTTTATAAAAAAGATCATCATTTTTATTCAAAACCAATTTATGATAGTAGTTTAGAATTGCAAACAATTTCTTCACTAACTTCCAAATCTAATTTTATGAAAGTTAAATTAGCAAACATCTTTTTTAATTATTTAAGTAAAAGTGCTGTTTTTAGTATAAAAAAACTATCAATAAAACAAAGTGAAAAATTCTTAAAATTTTATAAAACACAGACTAATAAAAGAAAGGTTATTAAAGTTTTAAATTTAAATCTTAAAAAAGAAATTAAACCTGAGAATTTTGTCATAAAAGCTCAAATAATTTGTGAAATACTTTTTAAAAACCCAATAATTCATCATTTTGTACATGAATATTTAAAAATTTTTCAAAATATATTTTCTCAAGAAGAAAAAGATAATTAAAACTAAGGATTTAAAATTAAGTAATGAATTTAAAAGAAATTAAAATTAAAATTAACGATGCCAATCAACGTTTAGATCGTTTTTTATTAAAAAATTTTCCTAATTTATCAAGAATTGCTGTTTATAAAATAATTAGAACAAAAGATGTTAAAGTTAATCATAAGAAAGTTGATCATAATTATTTATTAAAAGAAAATGATGTTGTTAGTATTTATGCTAAGGAGAATTTTTTAGAAAAAAAAGTTGACTTATCTTTTGCTAATGCTAAAGATGAATTAGATATTATATATGAAGATAAAAATATTTTAGTTGTTCACAAACCGATTGGTTTAATTGTTCATGAAGATAATAGATATAAAAATGACACTTTACAAAATAGAATTAAGAAATATTTAGTTAAAAAGGATGAATATCATCCTTTTGATGAAAGTGCTTTTGTTCCTAGCTTATGTCATCGTTTAGATTTAAATACAAGTGGATTAATTGTTGCAGCTAAAACTGCAAATGCTTTAAGAGTAATTACTGAAATGTTTAAAAATAATGATGTGATTCGAATTTATAAATGTTTAACATACAATCAATTACCTAAAAATAATGATGTTATTTATAATTTCATGTATAAAGCTGATGATAACACAATGATTGTACAAAATTTTAAAACAAAATTTAATAAAACAGCAATTACCAAATATACATATATAGATAAGTATAAAAAATATTTTATTTATGACATTGAACTATTAACTGGAAGAACCCACCAAATACGAGCAGTTTTAAATTTTTTGCATGCACCAATTGTTGGTGAACAAAAATACATCACTAAAGATATTGATAAGAATCAAAATTATATGTATCAATGTTTAGTATCATATAAGATCAAATTTATTAATTTAAAGAAATATCAATGTGAAGAATTAAATTATTTAGAGAATAAAGAATTTAGAATAAAAAATATTTGATTTTTAAAATAAAAAATCTATATTGAATTAAGAATTTAAAAATATAAAAATACTTAATTAAAATTTAAAATAAATATCTTGACATTTTCTACACTTTTTTAATATAATCTTCTAGTGCTTTTAAGCAATTAGTTAAATAATTTAAAAAAATAAAATATTTCTTGACATTTTAAAAAAAATTGTTTATACTATAATGTATTGTGTTTAAAGATTATTAATAATTAATCACAATAAGAATGTTCTTTGAAAACTAAATAGAATCCGTCAATTTTAAAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAATCGAACGAAGCCTTTTAGGCTTAGTGGTGAACGGGTGAGTAACACGTATCCAATCTACCCTTAAGTTGGGGATAACTAGTCGAAAGATTAGCTAATACCGAATAATAACATCAATATCGCATGAGAAGATGTAGAAAGTCGCTCTTTGTGGCGACGCTTTTGGATGAGGGTGCGACGTATCAGATAGTTGGTGAGGTAATGGCTCACCAAGTCAATGACGCGTAGCTGTACTGAGAGGTAGAACAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGGGCGCAAGCCTTATGAAGCAATGCCGCGTGAACGATGAAGGTCTTATAGATTGTAAAGTTCTTTTATATGGGAAGAAACGCTAAGATAGGAAATGATTTTAGTTTGACTGTACCATTTGAATAAGTATCGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAACGAGCGCAGGCGGGTTTGTAAGTTTGGTATTAAATCTAGATGCTTAACGTCTAGCTGTATCAAAAACTGTAAACCTAGAGTGTAGTAGGGAGTTGGGGAACTCCATGTGGAGCGGTAAAATGCGTAGATATATGGAAGAACACCGGTGGCGAAGGCGCCAACTTGGACTATCACTGACGCTTAGGCTCGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATCATCATTAAATGTCGGCCCGAATGGGTCGGTGTTGTAGCTAACGCATTAAATGATGTGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTTGACAATACACGTAGAACCTTACCTAGGTTTGACATCTATTGCGATGCTATAGAAATATAGTTGAGGTTAACAATATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTTTCGTTAGTTACTTTTCTAGCGATACTGCTACCGCAAGGTAGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATATCTAGGGCTGCAAACGTGCTACAATGGCTAATACAAACTGCTGCAAAATCGTAAGATGAAGCGAAACAGAAAAAGTTAGTCTCAGTTCGGATAGAGGGCTGCAATTCGTCCTCTTGAAGTTGGAATCACTAGTAATCGCGAATCAGACATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGGGAGCTGGTAATATCTAAAACCGCAAAGCTAACCTTTTGGAGGCATGCGTCTAGGGTAGGATCGGTGACTGGAGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTTCTTCGGAGTAAATTTTTAATTTACGTACTAATAAGTGTACATTTTTATTAAAATCCATATGAATATAAGCCACTTTTTTAAATATTTTTAAAAATTCATATGGTCGGATTCTATTTAGTTTTGAGAGTTTATTCTCTCCCATAATAATTAATTTATTATGGATGATCGATCTTTGAAAACTGAATAATTATCAACAAATCTTTCTAATCATTGACATTAAGTTGTCAGTGAACAGAAACTATATTAAATTAAATATAGTTAACAATCATAAATAAACAACTTTAAGGATTTATAATAAGTTACTAAGAGCTTATGGTGAATGCCTTGGGACAAACAGGCGATGAAGGACGTGCTAATCTGCGATAAGCAACGGGTAGCTGATAAGAGGCTTTAATCCGTTGATCTCCGAATGAGGAAACTCAATCTAATGAGAATTAGATTACTGTATAAATGAATTCATAGTTTAACAGAGCGATACCTGGTGAAGTGAAACATCTCAGTAACCAGAGGAAAAGAAAACGAAGTGATTCCCTGTGTAGCGGCGAGCGAAAGGGGAGTAGGCCAAACCGAATTTCGATTCGGGGTTGTAGGACTACAATATGGAATAGAGAATGATAGTAGAATTGGTTGGGAAGCCAAATCATAGAGGGTGATAATCCCGTATACGAAATCATTCTTTTACCTAGTAGGATCCTGAGTAGGGCGGGACACGTGAAATCCTGTCTGAATCCACCCAGACCATTGGGTAAGCCTAAATACTAGTTTGTCACCGATAGAGCATAGTACCGTGAGGGAACGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGAACCTGAAACCATAAGCTTACAAGGTGTTAGAGCACATTAATGTGTGATAGCGTGCCTTTTGAAGTATGAGCCAGCGAGTTATTATAGCATGCGAGGTTAAATCGTAGAGATGGAGCCGTAGGGAAACCGAGTCTTAATAGGGCGTTTTAGTATGTTATAATAGACGCGAAACGGGGTGATCTATCCATGGGCAGGTTGAAGGTGAAGTAACATTCACTGGAGGACCGAACCCACTTTCGTTGAAACGACAGGGGATGACCTGTGGATAGTGGTGAAATTCCAATCGAACTCCGTGATAGCTCGTTCTCGTCGAAATATTTTTAGGAATAGCGTTAGATTATTGGGAATATGGGGGTAAAGCACTGAATCTATGATGGCGCCACCTCGGTGTACTGAATAGAATCAAACTCTGAATACCATATTACCTATTCTAGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCGCGAGGGAAACAGCCCAGATCATTAACTAAGGTCCCTAATATATGCTAAGTGGAAAACGATGTTGAGTTTCATAAACAGCAAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGAGACTCTGCGCGGAAGATGTAACGGGGCTAAGCATATAACCGAAGTTATGGGTTACATATTATATATGTAGCGGTAGACGAGTGTTGTATATGGGGCGAAGGTAGACTGTGAAGACTACTGGACTTTATACAAGTAAGAATGCTGGCGTGAGTAACGAATGAGAGTGAGAATCTCTCAAACCGATTGACTAAGGGTTCCTGGGCAAGGGTCGTCCTCCCAGGGTAAGTCGGATCCTAAGGCGAGGCTGAAAAGCGTAGTCGATGGAAAACAGGTTAATATTCCTGTACCTACAAATTGTGTGATGGAGTGACGGAGAAGGTTATTATGTGCCGGTTATTGGATTCCGGTTTAAATAACAAGGTTAGTAAGTTGGCAAATCCGCTTACTATTAAGACCAAGTTATGAATACGAGCGACCCCTTCGGGCAGTAGCGAAGACATATACATCATGCTTCCAAGAAAAGCTTCTAGCGTTAAACAATTAGTAGTCCGTACCGAGAACGAACACACGTGGTCAAGGAGAATATCCTAAGGTTAGCGAGTTAACTACAGTTAAGGAACTCTGCAAATTAACCCCGTACGTTAGCAATAAGGGGTGCTCGCTGTAAAAGGTGAGCCGCAGTGAATAGCGAGGGGGGACTGTTTAACAAAAACACAGCTCTATGCTAAGTCGTAAGACGATGTATATGGGGTGACACCTGCCCAATGCTGTAAGGTTAAAGAAGAATGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAATGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCAACTGTAGACTCGGTGAAATCCTGGTGAGGGTGAAGACGCCCTCTTGGCGTGATTGGACGGAAAGACCCCATGAAGCTTTACTGTAGCTTAATATTGGGAAATTTTATTACTTGTAGAGCATAGGTAGGAGACTGTGAAGTATACTCGCTAGGGTATATGGAGTCAACGTTGGAATACTACCCTTGTGATAAGATTTCTCTAACCTGCAGCCATGAATCTGGCTGGGGGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCCAAAAGGTAACGGAGGCGCGCAACGGTACCCTCAGCACGGTTGGAAATCGTGTATAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAAGTCGAACAGGTAGGAAACTAGGTCATAGTGATCCGGTGGCTCAGAATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCTGGGGATAACAGGCTGATAGTGCCCAAGAGTTCATATCGACGGCACTGTTTGGCACCTCGATGTCGACTCATCTCATCCTGGAGCTGAAGCAGGTTCCAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTGTCATGCCCGTAGGAAGATTGAGAAGAGCTGTTCCTAGTACGAGAGGACCGGAATGGACACACCTCTTGTGATCCTGTTGTCGCGCCAGCGGCACTGCAGGGTAGCAACGTGTGGAATAGAGAAACGCTGAAAGCATCTAAGTGTGAAACTAACTTCAAGATTAATCTTCCCTTTCTTAAATGAAGTAAGAATCGTTAAAGACTATAACGTTGATAGGTCGGATGTGTACGCACGGTGACGTGTTTAGCTAACCGATACTAATAATTCGAGGACTTATATAAGTTCTTAGTTAATTTTTAGAAGAATAGTTGATAATATTTAGTTTTCAGATGTCGATCATAATTGTGTAGTGATTATATCAGAGTGGAAATACCTGTTCCCATCCCGAACACAGAAGTCAAGCACTCTAGAGCCGAAAATAGCGCAAGTAAAATAGGTCATCGCTACGCTAAAACCAACCTTGAAATTTAAGAGGTTGGTTTTTTTATACTTAAATAATATATTTTTTAATATATATCAGTCTAATTTAAATAAATTAGTATATAATCAAAATGTGACAATCATTTATGATTAAGGATTTTATATTTAAATATGTTAAAGTTAATTTGATGTCAAACATTGAATGGTGGTATATCAAAAAATAATAAATTACCATGATATATAAAAGAAGAATTAGATCATTTTTATAAAACAACAAAAAACCATAAAATTATAATGGGTAAAAACACTTTTGATTCATTAGATCAAAAACCATTAAATAATCGAACAAATATCATTTTTTCTTCAATTATGCAAACACCAGAAGACGAATCGTATTTTGTGACAAATGATTTTCAACAAGTATTAAACGATGCTAAAAAAGAGGATATTTTTATTATTGGCGGCAAGGAATTATTTGATATTTTTTTAGCTTATGCAGATGTTTTAATTGTTTCTGTATTAAAAGATTATTATGATTGTGATCTATACATGAAAGTTGATTATAATAATTTTAATTTAGATAAACAAGATGTTTATGATAATTTTATTGTTAATTATTATTCAAATAAGAAGGAAAAATAAAAGATTTATGACCAAAAAACTGATTGTTTTTGATTTTGATGGTACAATTATGCACACGCATACAACTATGAGCCTATCTATTATTGGTGTTTTAGAATTTTATAATCATCATATACCAAGTTTAAAAGAAATGAACGATCTGTTAGGTAATCTTTCTATGGTAAATATTTTTAAAAAGTATGCTAAACACGATCTTTTAAATATCGAAATTGAAATGATGATTTCTAAATATTATGAAATATATGAATCTTCGCTTTTTATGATCCATAGTTATTTTTTTGATGGTATTTTAGACCTAATCAAAAAATTAAAATCACTTAATAATAATGTTAAATTAGCAATTTTATCAAATAAACGATCTTCTTTATTAGCAACAATGGTTGATTATTATAATTTACGACCTTATTTTGATTATATTTTTGGTGCTGAAGATGTCGAACAAATGAAGCCAGATCCTAGTGGTTTACTAAAAATTATGAACAACTGTAATGTTGATCATAAAAATACTTTATTGATTGGTGATAGTATAGCTGATTTAAAAGCAGCTATAAATGCTAATTGTCATTTTATTTTAGTTAATTGAGAACCACAATATCAAAAGCACATGGAAACAATTATTAATTTAAAACCTCTTATTGTTAAAACAATTGATGAACTAGAAACACAAATTAACCACTTTTTATATTAATGAACAAAAATGGCACAAAAAAGACTAGTTTATTATATAATATATAAGTGCTAGTTGCAGATGTAGTTCAATGGTAGAACGCAACCTTGCCAAGGTCGAGACGCGAGTTCGATTCTCGTCATCTGCTCCATTAATAAATAAAAAAGCAATTCTATTATGAATTGCTTTTTTTATTCTTTACTTAATTATTAAAATTATTTAGTACCGAAAATTCGATCACCAGCATCACCTAAACCAGGAGTGATATATCCATTTTCATTTAATTTATCATCTAATGCTGCGATATAAACATCAACTTCAGGATGAACTTCTTGAACATATTTAAGACCTTCTGGAGCTGCAACAATACATACAAATTTAATGGATTTTGGATTGTATTTTTTAATAATATTAACAGCTTCAACAACACTACCTCCTGTTGCTAACATTGGATCTAAAATAATAACATCTGATTCAGAAATATTTTTAGGAAATTTTTTAAAATATTCTACTGGTTTTAGTGTTTGTTCATTACGATATAAACCGATATGACCGATTGTGGCTGTTGGAATTAAACTTTTAACACCATCAACCATTCCAATTCCAGCGCGCAAAATAGGTATTAAACAAATTTTATTTTTTAATTTATAACCTTTTGCAACCTTAACAACAGGAGTTTCAATTTCTATTTCATTTAACTCTAAATCTTTTGTTGCTTCATAAACCATCAATTGTGTAAGTTCTTCTAAATTTGTACGAAAAACAGTAGAAACTGTTGAAACTTTTCGCATACGTGTTAGTTTATCTTTAATTAATGGGTGATTAATTATTTTATGCATTTTTAACTCCTTAAATCATTTAACTTGTATATATAATTTTAATATTCGTTCTTAATTATAAAATTTATTATTATAAATAACTTAATAAAATGAAAAAAACTAGCAAAATGCTAGTTTTATTACATTAATATATGTCATCACACATAAAAAGATATTGCTATTGCTGCTCCATCTCTGGCCTGACAAGGTTCGCTACTTAATATTGTAATGACATATATATTATAAATAAAATTGATTAAATTTTTTAATCTTTATTTCATAAAATTAAAAAATAATTTCGTTTACCTTTTCTAATAATACTAAATTTTTGATTAATAGCATCTTGTTTCTTGACTAAAAAATTTTCATCATTTATTTTAATATCATTAATTAAAATTGATCCTGTATTTAAAAAATCTCGTGCTATACGGTTACTTGATGAAATATTAGCTAAATTTAATAAATCAATTATTTTAATTTCATCTTTGTCTAATTTTGTTGCAGGTAAACTCTTAATTGCGATTTCTAATTCTTCTTGATTTAATGTACTAATACTACCATTAAATAGTACTTCGCTAATATGTAATGCTTGTTCATATTTTTGTTGTCCATGTATATCAACAACAACAGCTTTAGCCAATGCTTTTTGTGCAACTCGTAAAGCGGGATTATTTTTATGAGCTTGCATTATTTTTTTTATTTCTTCAACATCAATTAATGTTAAAAAATTAAGTAATTTTTCTACATCAGCATCGCTTTGATTAATTAAAAACTGATACATTTCATAAACAGTTGATTTATTTTCGTCTAAATAAATAGCACCTTTTTCACTTTTACCAAATTTTTTACCTTCTGAATTTGTTAATAAATTAATTGTCAATCCACAAGCGATATTGTCATCACCTAAATTTTTTCGAATCATTTCAATTCCAGTTGTAATGTTACCCCATTGATCACTACCACCAGCTTGAATGGCTATGTTATCATTTTTATATAATTGTAAAAAATCATAACCTTGTAGCAAATTATAACTAAATTCTGTATATGAAATACCAACAGATAAACGAGAATTAATAATTTCTTTTTCTAATAAATAATTAATGTTGATTAACTTTCCAACATCACGCAAAAAATCTAAAAAAGTCATATTTTCATAAAAAACATAATTATTAATAACTTGAGAATTAGTAAATTTTTCAAGTTGAAATTTTATTTTTGCAATATTTTTTTCTAAAATTGTTTTATCTAATAAATTACGTTCAGCGGATTTACCACTTGGATCACCAATCATTCCTGTAGCACCACCAACAAGCGCTAATGTTTTAATGTTATATAAACGAAAGCGTTTTAATAACATTATCATAATATAATTACCTAAGTGTAAACTATCAGCTGAAGGATCAAAACCTACATAAATTCCTTTATTTTTCTCTAAAGCTTTTATTAATTTTTCTTCATTAGTTATATTATTGATTAAGTTTCGTGCTTTTAAATCCTTAATTAAACTATGCATAGATCTATCTTTTCCTTAATTAATTACTTGATTTATAATTATATATTTTAATACTATATTACCTTTAAATCAAATTAAATTATTGATTTGTTTTATAATTAAATGTTAAATAGCTTTACTTAAATAAAGGATGGCTTTATGAGAAATAAAAAAGAAAATGATAATGTTATTAATCCATTATCTAATCCAGATGAGAACAAAAAAATTAATATAAAAGACTTATCAAAAGATGAGCGTAAAGAACTTAAAAAACGAATTAAAGAAGTTGAAAAATTAAACAGAAATCACAAAGGTGACATTGATATGCCATCACGTGATCCAAATAATATTATTGAACTTCGTGATGTTAAAAAAATTTTCACAAATGGCTATTTAATTAATGAAACACTCAAAGGTGTTAATTTAGATATTCGTAAAGGTGAATTTGTAATTATTTTAGGACCATCAGGTTCTGGTAAAACAACATTAATGAACATTATGTCGGGACTTGATCGTGCAACTGATGGTGATGTTCGTGTTTGTGATAAACAACTAATTAATATGAATCAAAATAAATTAACTGATTTTCGTAAAGAATATATTGGATTTGTTTTTCAACAATATGGATTATTACCAACACTATCTGTAGAGGAAAATGTAGAGATTGGTGCTGATTTACAAGTTGATAAAAAACGCCGTATTAAACCACAAGATGCATTAAAAGCAGTAGGGATGCTTGATTACGCAAAAAAATTTCCACATGAATTATCTGGTGGTCAGCAACAACGAGTCTCAATAGCCCGTGCTTTAGCTAAAAATCCTATTATTTTATTTGGCGATGAACCAACTGGTGCTGTTGATGAAACAATGTCAAAAATTATTTTAAAAGAATTTGTAAAAGTTAACCAGGAATTAAAGACAACGGTAATTATTGTTACCCACAACCCAATTTTTGCTGAACTTGGAACTTTAGTTATTAAGGTTAAAGATGGTAATATTAATGAATTAATTCGTAATGATCATCCAAAATCAGTTGACGAATTAAAATGAGATGAACAATAAGAGGACTTAGATTTAAATGAATTTAATATCAAAAATATCCCCCCAAAACCGAATCTTAAATCGCGCTCGATTGATTGCTGAAGAAGTTTTAAAAAAAGAAGAAGAATACGAACATTTTAGCGATCAAGAATTAATTAATAAATCTGATGATATTATTGAATATTTAGCAAATAATAACCCATTAGATGATAAATTAGTAGAAGCATTATGCATTATTCGTGAAGTGATTTATCGAGTTCATAATAAAAGAGCTTTTAAAGTTCAAATTATTGGTGCAATTATTGTTTATTTTGGTGATTTTGCTGAAATGATGACTGGTGAGGGTAAAACATTAACTTTAGTTTTAGTTGCTTATTTAAATGCACTTTATAAAAAAGGTGTTCATATGGTAACTGTTAACGAATATCTAGTTAAAGTTGGAGCAGAATTTGCAACACCTGTTTTAAATTTTTTAAATATGTCTGTTGGCCAAATTACAGCTAATATGAATGAGTATGAAAAACGAAATAATTATAATTGTGATATCACATATACAACTAATTCAGAATTAGGCTTTGATTATTTACGTGATAATATGGTAACTAATTATGCTAATAAAGTTCAACGGGGCTTATGATTTGCTATTGTTGATGAAGGTGATTCTGTTTTAATTGATGAAGCTCGGACTCCTTTAATCATTTCTGGTGAACCTCAAGAAGAAATTGGAAATTATGTTAAAGCTGATCGTTTTGTAAAAACATTATATCCACAAGATTTTACTTTAGATCCAGAATCACAATCAGTAGCATTAACAGAAAGTGGCGTTGAAAAAGCACAAAAGTTTTTCAATACTAAAAATTATTATAATTTTGAAAATTCAGATATTATCCACAAAGTTACTAATGCTTTAAGAGCAAATTTTACTTTTTTTAATGGACGCGAATATATTGTTAAAAAAGATGATGATGGTGAGGATATAATTGCTTTAGTAGATCAATCTACTGGTAGAATTATGGAAGGAAGAAGTTATAGCGCTGGATTACAACAAGCAATTCAAGCTAAAGAACAAATTAAAATTGAACCCGAAAATTTAACAGTTGCCACAATCACTTATCAATCATTATTTCGTTTATATAAAAAATTAGCAGCAGTTTCAGGAACAGCTATTACTGAAGCAGAAGAATTTTTAAATATTTATAATATGGTTGTTGTTACAATCCCAACAAATAAACCAATTAAAAGAATTGACCATCCTGATTATGTTTTTGATAATAAAAGAACAAAATGGAAATATGTAATTGCTGATGTTATTCGTCGTCATGAAAATGGCCAACCAATTTTAATTGGAACTGCTAGTGTTGAAGATTCAGAAATTTTACATCAATTACTAGAACGAGTCAATATTCCTCACGAAGTTTTAAATGCTAAAAATCACGCACGCGAAGCTGAAATTATTGCTTGTGCAGGAGAGTATAAAGCAGTTACAATAGCAACAAATATGGCAGGCCGTGGGACTGATATTAAATTATCTCCAGAATCATTAGAAGCTGGAGGATTATGTGTTATTGGAACCGAACGAAGTGATTCACGTAGAATTGATAATCAGTTAAGAGGACGTGCTGGACGACAAGGTGATATTGGCGAATCACGTTTTTTTATTTCAATGGAAGATACTTTATTTAGTCGTTTTGCAACCGATAATTTAGCAAAAGCTGATGATAAATTAAGTGAAGATGTTATTTCAACAAAATTCTTTACACGATTGCTAAATAATACACAAAAAAAAGTTGAGTCGTTAAACTATGACACAAGAAAAAATCTAATTGATTATGATCATGTTTTAAGTAATCAACGCGAATTAATTTACAAACAACGGGATAAAATTCTTATTTCATCAGATAATAAAGATATTTTATATCGAATGTTAGATAGTGTAATTGATGATTTAATTTATCAATCACACAATAAACCAAATGAAGACATTATTGACATAAAAAAATTAATTGATTTAGCAACTCAAAACATTTTTTATGACAATTATTTAAATCAGGACGAATATTATGGGCTTAAGTTTGAACAAATTAAAACTAAATTAAAAAAAGACTGTATTAATTTTTTTGAGCAAAAAGAACAACTAATGACACCGACAATTTTTAATCAAATCTTATCAGAAATTATGATTAGTAATATTGATGAAGAATGAACTAAACATTTAGATATTACTTCTAAAATTCGTGAGGGAGTTAATTTACGTGCTTATGAACAAAAAGCACCATTAAATATTTATGTTGAAGACTCAGATAAATTATTTGAAAAATTAAAACATAATGTTGCTTGAAAAACAGTATGTTCAATAGGAAAAATAAATTATGTTCATCAAGATTATAGTGATTTAAATAGTGAATTTATTGTAAATGATAATGAAATTAATGAAAATAATAATTCAATTGATTTTGAAAATTTCAACGAATCAATACCAACTGATCAAACAATTCAAGAATCATTTGATGATAATCAATCAGATAATGAAGATGATAAAAATAATAATTAACAATGGATGATTTTAAAAAATTTGAATTAATAAGCGATTATAAACCAGCTGGTGACCAACAAAAGGCAATTGATGAAATGGTTAATAATATTAATCAAGGTATTCAACGACAAGTATTATTAGGGGCAACAGGAACAGGAAAAACATTTAGTGTGGCTAATGTAATTGCAAAAACCCAATTAAAGACTTTAGTTCTTGCTCATAATAAAACATTAGCTGCACAATTATTTGCTGAACTTAAAGAAATGTTTCCTCATAATAAAGTTGAATATTTTGTTTCATATTTTGATTATTATCAACCAGAAGCTTATAAACCTATAACTGATACTTATATTGAAAAAGATTCAGTAACAAATGCAGAAATTGAGATGATGCGTTTATCAACTATTAATTCATTGGCTACAAGAAATGATGTCATTGTTGTTGCTTCTGTTGCATGTATTTATGCATCTGTTTCTCCTATAGAATTTACTAAAAAGAATTTATATTTACATGTTGGTGAATTAATCGAATTTGAACAAATTAAATATAAATTAGTTCAATTAGGTTATGAACGAAAAGATTATGATTTAGTGCCAGGAACATTTAGGATTCGTGGTGATTTAATTGAAATTATGTCTGGATATAGTGATAAATTTAAAATAAGAATATCAATGTTTGGAAATGAAGTTGAATCAATTGATCTTTGCGATCCAATTACTAATAATATTATTTCTAAAGAACAAAGGTTTATTTTACGAAGTGCAAGTGAATATATTTTTGATGATTCGCGTTTAGCAATAGCGATTGAAAATATTAAAAATGAACTAGATGAACGAGTTAAATTTTTTCTAAAAAACAATCAATTAATTGAGGCGCAACGGATTGAGCAACGAACTAAACAAGATCTTGAAAGTATTGTAGAGTTTGGTTTTTGTAGTGGAGTTGAAAATTATTATCGTCATCTTGAACATCGTGAACCTTTTCAAACACCATGGACAATTTTTGATTTTTTTTCATATAACAATCAAGATTGATTATTAATTGTAGATGAATCACATATTTCATTACCACAAGTGAAAGGGATGCATAATACAGACCGTAGTCGTAAACAAACATTAGTTGAATACGGATTTCGTTTACCTAGCGCTTTAGATAATCGGCCTTTAAATTATGATGAATTTAATAAAAAACTTTCCAAAACGATTTATGTTTCTGCCACACCAAACGATGAAGAAATTGCGTTATCAGATAATCATATAGTTAGTCAAATTGTTCGTCCTACAGGATTATTAGATCCAATTATTGAAATTAGAAAAACAGAACACCAAATTGATGATTTAATTAATGAATTGATGCTTTTAAAAAACAAGAATCAACGAGCATTTATTACTGTTATGACAATTAGAATGGCTGAGGATTTAACAAATTATTTAAATAATACAAAAATTAAAGCTGCTTATTTACATAATGAATTAAAAACACTAGAGCGTAGTGTTATTATCAATAAATTAAGAAAAGGAATTTATGATTGTGTTGTTGGAATTAATCTTTTAAGAGAAGGATTAGATGTTCCAGAAGTTGCAGGTGTTTTTATTTTTGATGCTGATAAACCTGGTTTTTTTAGAAGTGATAAATCTTTAATTCAAATTATTGGACGTGCAGCACGTAATGCTGATGGTAAAGTAATTATGTATGCTGATGTAATAACACAAGCCATGCAAACCGCAATCAATGAAACAAAACGACGACGTGAAATTCAATTAGCTTTTAATTTAAAACATAATATTATCCCTAAAACAATTATTAAACCAATTCACGAAGATTTAAGTGGCCATGATTATAAACAAAATGCGGAATTATATGCTGCAAAAGCTTCAAAAAACGAGTACAATCAAAAAATCAAAGAATTAAAGAAAAAAATGGAAGAAGCTGCAAAAAAACGTGAATATGAAGTTGCTGCTCAATATCGCGATATGATTGTTGAATTAGAAGCCATTAAGCAAAGTGTAAAAAGCAAATAATAAATATATAATTATGTTTATTTTAATATTAGTTTTTTAATTAAAATTTATACTAATAAAGATTTAAAAAGGTTACTTTACGTATGGATAAAATAAAAGCTAAAATTGACGAATTAAAACAAAAACTAGACCAATGAAACTATGAATATTATGTTTTAGATGATCCAAGTGTGCCAGACCATGTTTATGATCAAACAATGCGGGAGTTAATCGAACTTGAAAATAATTATCCTCAATTTAAAACTAACAATTCACCAAGTGTAAAGGTTGGCGGCTTTGTTTCAGAAAAATTTAATAAAGTAAAACATAAAAGACCAATGTTATCACTTTCAAATGCTTTTAATGATGATGATTTAAAAAAATTTGATCAAGATAATCAAAATGCTAGTGTCGATTTAAAAGGGTACGTAGTTGAACCTAAAATTGATGGTTTAAGCATTTCTATTATTTATAAAAATGCCAAATTACACCAGGCAATTACAAGAGGAGATGGGATTAATGGCGAAGATGTTACTTCCAATATTTTAACGATTAAAGACATTCCTCACTATATTGATCAAAAATATAAAGACTACGAAATTGAAGTTCGTGGTGAAGTCTATATGGCTTTTCATGATTTTTATGAAATGAATGATAATTTAGAAGAAAGTGATAAAAAATTTGCTAATCCAAGAAATGCTGCTGCTGGAACATTAAGAAGTCTAGATAATTCAATTGTTGCAGAACGTAAATTAAGTGCTTTTATGTATTATTTGGTTAATGCACAAGAACTAGGAATTAAAACGCATTATGAAAGTATTCAATTTTTAAGAGATAATAAATTTAAAGTTAGTGATTTAATTGTTAAAGTCGATACAATTAATGAAGTTATTAATCAAATTGATTGTTATACTAAAGTGCGTGATAAACTTTCATATATGATTGATGGTATTGTTATTAAAATTAATAATTTAGAAGTTTATGATGAGATTGGTTATACTTCTAAATTTCCTAAATGAGCAATTGCTTATAAATTTCCAGCTAATGTTGTTAGCTCACAATTACTAGAAATTATTAACGATGTTGGGCGTACAGGAAAAATCTCATATGTTGCTAAAATTAAACCAATTTTATTAGATGGTAGTATGGTTGAATACGCTACTTTACATAACTTTGATTTTATTAAAGAAAAAGACATTCGAATTAATGATGAAATTAAAATTTATAAAGCAGGGGATGTAATTCCTTATGTTGATGGTGTTGATTTATCAAAACGTTTAGCAAATAGTGTACCTTATGAACCAATTATAAATTGCCCTTCATGCCAATCAGTTTTAGTACGTGAAAATGATGAAGTTGACCAACGCTGCTTAAATATTTATGGATGTAAAAAAATCAATATTGAAAAAATCGTTTATTTTGTTTCACGTAATTGCATGAATATTGAGGGAATGAGTGATGCCATTATTAATAAATTTTATGATGCTAATTTAATTAAAAACATTGCAGATTTATACTATTTGCAAAAACACAAAGAATTTATTTTAACTAGTGATTTTAAAATTAAAGAAAAAAGTTTTAGTAATTTAATTAATAGTATTAATAATTCAAAAAAATGTTCTTTAGAATTTTTATTAACAGCTTTTGGAATTCGTCATGTTGGACCAAATTTAGCTAAAAAAATAGCTAAACAATTTAAAACAATGACTGCTTTAATGCACGCTAATTTTGATGAATTAACTAATGTTGATGCATGTGGGGAAAAAGCAGCCTTATCATTAATTAATTGGTTTAATGATGAGCATAATGTTTCTTTAGTTAATCAATTACAACAAGTTGGTGTAAATATGGAATATATTGATGATTTTATTTATGATGATAACATCAATATAATTGATGAATATAAAAATAAAACTTTTGTTATTACTGGTTCATTTAGTATTAGCCGTGATGAAATTAAAACTATTTTAGAGAAATATTATCATGCAAAAGTTAAAAACAGTGTTTCTAAAAAAACAGATTATGTTTTAGTTGGCACTGAAGCGGGTACGAAATTAGAAAAAGCTAAATTATTAGGTGTGAAAATTATTGAAAATGAATTTTGAAAAAAGGATAATAATTTTTAAATTACTTGAATATTAATAATTTTAAGTTGTTTTTGCTGCTAAATTAAAATTATTGTAATGTTTTCTATGATATAGAAAATAATAATTTTGTAATAAATATGCTCTTAAAATAAAATTTAAAATGTATATATTTTATAATTAAGAAAGATAAAACTATTAAAATAATTGTAATGCATTATATTAAAGTTAAATTAACGCTTTAATTAAAGATAGTAAAGGTATTGGTGTAAAAATTATTGAAGATAAATATAACAAAAGAAAAAACAATTTTTAAAAAAAATTATAAAAAAAAACATAGTATATATTATTCAACATCTAAAATTATTAAAGACTACACACTAAGTGCAGTAATGTTAGGTTTAGGAATTTTGGTTGCATATTTAAGTCACTTTATTCGTTTTAATTTCTTAGCTTTTGATTTTTCTTTGTTTACACTTGTTTATTTAATTTATAAAGTTAAATATCGCTTTGTATATTTAGTTACAATTTTATTAAGTTTAGCTAATTTATTGCACGGAAGTCCTTCATGGATCGGAACAATGGTTTTAAGTTTAAATAACTTATTTTTTGTTACTATGGTTATTCTTTTTAAAAAGTTATTTATTCCAAAAAGACCAAAATTTTATCTTAATTTATGAGTTTTAGTATTAAGTTCATTCTTAACAACTGTTTTTAATGTGATTATGAACGGTATTCTTTACACTCCACTTTATTGATACACTTTTAAAATTACCCCAACACTGAATTTTTTAGAAGTACAAAAAATTTATGAAAAGCACCCGACTTTATTTTTGTTAAATATTAAATCATATTGATTAGGAATTATTGCTTTATATACAAGTTTTAATTTAATTAAAAACATCATTATTAGTTTTATATCATTAACTATATTAAAAATTTTTGCAGATAAAAAAACGATTATTACATATTAACAAGGAATAATTTATGAAATTATATGATTCTTATTCTAATCAATTAGTAGAAATTAATGATGAATTAATTTCAATTTATAATTGTGGACCAACCGTTTATAATCATATCCATATTGGCAATGCTCGGCCTTTAATTACTATGGACGTGTTATATCGTTTTTTAAAAAAACATAATATCAAAACTAAATATGTTTTAAATATTACTGATATTGATGACAAAATCATTAATTATGCTCTAGCTAATAACCTAAAAGAATTAGAGGTTAGTGAATATTATTTTAATGAATATTTAAAAATTAAAAAAGCTTTAAATACACTAGAAATGATTAATCCTAAAGTATCAACACATATGGATAAAATTATTGATTATATCCAAAAATTGATTGATAAACAAGCAGGTTATTTTATTGGTGATGATGTTTATTTTGATACAAAAAAAGCTTTAAATTACGGACAATTATCGAAACGTGATTTAGAAAATGATATTGTTGGTATGCGTATTGAAAGCGCAGCTAATAAACACAATCCTAATGATTTTATTTTATGAAAGAAAACAAATAAAGGTATTATGTGAAATACACCTTGAGGAATCGGTCGCCCAGGTTGGCATTCTGAATGCTCATGTTTGATCAACACTTATATTGGTGAACAAGTTAGTATTCATGGTGGTGGAATTGATTTAAAATTTCCCCACCATGAAAATGAAAATGCTCAAAATCAAGTTTTATATAATAAAAATTTAGCAAAAGTATGAATGCATTTTGGATTAGTTAATATTAATAATGAGAAAATGTCAAAATCTTTAAATAATTTTATTTTAGTAAAAGATTTATTAGCTGAATACGATTATCAAGTTGTGCGTTGGTTTTTTTATCAAGCAGATTATAAGCAACCAATTAAATTTTCGCATGAAATCATGAAGCAGAACGAAAAAGAAATTCTTAAAATTAAAAATGCTATTTATAACGCAAAAAACTATTTATACTTCAATAATCAACTAAAATCATTAACACAAATTGATCATTTTGAGCTTTTTGATGAACGCATTAATGATGATTTAGATTTTGTAGGTATTGTTGATCTAATTCATATTAGTGTTAAAAAAATAAATATTTTAATTAAAGAAAATAAGGATATGAATGAATTAAAACTGAATTTAACTCAATTATTATATATGTTAGATATATTGGGTATTAATTTTGTTGATTTGCATAATGATGAGAATTTAGCATTATTAAATACTTGAAAAAATTACGTTGATAAAAAAGATTATGTTAAAGCTGATGAATTAAGAAAACAATTAATTAATATAGGAATTTTATAAAATGAATTATAATTTTGGCAAAAAAGCATTATTAGATGCAATTGGTAACAAACAATCAATTTCAAAAGTGTATTTATTATCAAAAAATTATGAGTTAATTAATTTAATTAATAAAAATAAAATTACTTATGAAATTGTTAATGAAGCATGATTTAAACAATTTGATCATTCTTTAAACCATCAAAATATTGCATTTAAACTAGTCAAAAAAAACTTTCAAAAAACGAGTTTAGAATCTTTTTTAAAATTAATAAAAGATAAAAAAAATGCTTTAGTTTTAATACTTGATGAAATTGAAGATCCTCGCAATTTTGGAGCAATTATTAGAACTGCTAATGGTTTTGGTGTGGATGCTATTATTTATAAAAAGCATCACCAAGCAAGCATTAATGATTTAGTAATTAAAACAAGTATGGGTGCAATTAATTATATTTATATGATCGAAGTCAATAATTTATCAAATACAATAAAAAACCTTCAAGATGAAGGTTTTTGAGTTTATGCGACAAGTTTATTAAAATCACAACCATATGAAAAAGTTGATTATGCTAATAAAACTGTTTTTATTGTAGGCAATGAAAATAAAGGTGTGAGTCCATTAATTTTAAAAAATGCAGATCAAAGCATACGAATTGATATGTTTGGTGATGTACAATCTTTAAATGTTGGTGTAGCAACAGGAATTATCTTAGCGCATTATCGCACTAAAATAAAACCCTAGTTATTTTTTTATGATGGTTTATTTTTGTCTGTACATTTTCCAGTTGCAAAACACTCTGCAATACCAAATCAGACTTTATTAACTTCGTTGTGTTTTGACTTTCAGATTTTTTGATATTCAGTTTCGTTTTTATCATTTTTTAATGTAAAAAAATCAACGAACATTTTAAATTTTTGATTAATTTCCATGGGAAAAGTTAATAAAGCTTTTTCTGTTTGCTCTATAAATTCATTTCTTTTTTGTTCTTCAACAATTCGATTAAAGGCTTTTGTTAGTGTATCATCTCTACCATATACAATTCACTTAAAATATTTTTTAATTTGATCTTCAAATTTAGTAAAAAAGGCTTTAGTTTCATTAGTTAAAGATAATAACACTAAATTAAGTGAATTTAGGTATTGGTCTAGGTTTGAATTGATAGTAACGTTTTTTAATTTATTAAAACTACTTTGAATAGATTTTGTTAATTCTAAATATTCTTGCGGATTAAACTTTTCTCTTTCTTGATATTTTTGATCCTTAATAAAGTCCTGGTTATTAATAAATTTTAGAAACTTATTAATGTATGAATCTTCAATATTAAATAGTTGATCTATTATATTTTTAAATTTATGATCTAATTCTTTAATGAAAAGGTCTAAATTTTTTAATTTTAATTCAAGTTCTTCATTTTTGAAATCTAAAAGATTTTGATTTTGATTGATAATAGTTTGAATTTCTGTTGTAATTGGAGTGAAATTAATAATTTGATTAAAAAATGATTCTTTATTTCCTTTTAATAAATTATGATTAACTATTTGATTTAAATTTTCTAATTTAATGTTAATTTCTTTATTAAGATAAGCGTTTTGTTGCTTTTCTTTTTCTTTTTCTTTAGAAATTTTCTCTTGAATTTCATCTAATAATTTTTTTAAATCATTATAATTTTTAGTAATGACATCTAAATTATTTTGTTCATTAATTTTTAAAACTTTATTAATTTGATCATTTAAAACAGAAGCCTCCTTTTTAAATAATTCATTAGTTTTAATTTCATTATATATTTGTTTTGCATGTTGAGATAATTTTTTTAATTCAGTTAAATGTTGCTCTTTTTCTTTATTAGTACAAGCTACAATAACACTTGAAGTAGTAACTAAAAAAGATGTAATAACTAGTGATTTAATAAGAAGTTTATATTTATTTTTTTTCATAAGATTCCTTTCTATTTATAAATATCTTCATATTTTTCTGGATATAAATAGGCTAATTGTTTGATTTGTTGAATTAAAGATGTACGATTAGCGAAATTGTTTTTATCTCCAAAAAATCCAGAAATTAGAGAATCAAAAACAATCATACCTCCGGCTGTAAATTTTGCATTTTCATGGGCGCCAAAAGAAATCATTCCAACTAAATCACCATTTTCATCATAAACTACTGATCCTGATGAACCAGAACCAACATCAAAATATTGATTACTAATTTTAGTTGTCCCTACTGTTGATAAGTAATTATTAGTAGTAATAATATTTTCTTGGATTTTATCATATTTATTAATTTCATATTCACGATAGCGTGGAAGTTTGCCAGCATTAAATTCATTAATTGTCTCTGTTCTAGGGAAAGTTGACAAGTATCATTTTACATTTTGATTATCATCGAAATACTTAGTTTGATTACTTAATTTCAAAGGTTTTAGATCCTTAAAATTAATTATATGTCTAATAACACTCTGTTGTTCTTTTGTTAAATCATTAAAAGCAGGTGAATTTTCTAATTGACCATATTTTTGAAAAAAAGGTTTTAAATTAATGGTAATTAATGAAAAATCTAAACTTGCATTAGGATTAAAAAAACCTAAAGCGTTAACATGAGGGGATAATTTATTTTTTACTTGATGATCAAAATTAATGATCGATTCGATACTAATTTTTTCAGGAAAATTTAAAAATTGAAATTGATTTTGATTAGCTTCAAGTTTGCCATTTTTTTCAAGTGCATTGTAATAAGGATTTTTTTTATCATTTAATGTTTCATTTTCATGAATAATTTGGGGAACTAGTGGATCAAAATCTCCATTAGAACTATTACTTGATTCAAAGAGATCTTTATTACCTGTTTTGTTAATTGATTTAAAAGCTTGCGTACTATTTAAAACATGAGCACAACTAATTAGATAATAGCGATAATCATCATCATCATTAGGTTTAACCTTCCCTAGAACATTTCATGTACCATCTTGAATTAAACTTAATGAAATTACACGATCACGAGCATTTTTAATTGGTAAAAAATTCTGTTCATTGTTTTTATAAATTCTTGTTGTATATTGATTATTAGATTTATCATCTTCCATTATATCTATAGTAGTATTATTTAAATAATAAATATTAAAATTAGCCTTTGTTAAAAAATAAGATGAATCTATTGAAGAATTATGTTCTCAAATATTATCAAAAACACTAATGTTATTTTTAGAAATAATTTTAATTTTTAATTCATCATTTATATAAGATATTTGAAAACGAAAATCTTGCTTAATTTTTTTGTAGAGTTCATATTCGTACTCTTGATTTCCAATACCATAATTTAAAACAAATTCAGCATCTTCAATAACTTTTTTATTTTTTAAAGTTTTATAAGGGACTGCTAATGAAATTTGAGACCTTAACATAAGGTTTTTTCACAGGTTAGTTTCTTTGATGAGATTTGGATCTAAACCAAAAGTAATATTAAAAACTAAATTTTTTTCATTTACAAGATTTAAATTTTTAATCTTATTTATTTTTTTTGTTAATTGAAAATAATTATCTTTATCTTTTTTAAAGATCAAATCTGGATTATCTTTTAATGTAGTTTTATGTAAATTTTTGATTTCATTATTATTTACAAGTATAAAATCAGCTTTTTTAGTTATGAAATCATAAAAACCTTCGTTTGTTTTATTTTGATAAAATAAGTAGGTATTTTCATATTGAACTAATGAAATAAAATTTCGCAATGAATAATCTAAATATAAATTATTTTGATTAGATGCTCAAAGTTTATATAAGTCATTTAAAGTTAAATTTTTATTCTTTAAAGTTTGATATTTTGTTGAAAAAAACTGATTATTATTGTAATACAAATCAATAATTTTACGATCATTATATATTATATTTTTTACAAAAAAAGTACTTGTATTTAATTCTTTTACTTTAACAAAAGCAAAAGGCGTTAAACTATTATTATCACGAACACTAATTCTGTTTAATTTGTATAAATATCCATTATATAAATGAGGGATTTTGAATTTTCATAGCGATTTAAAAGGAACACGAACATACTGAATTGGTGGAGTATATTTTGCAATTAAACTTTGATTTTTTTTAGCTTCGCTATAATTTTCGTCTAGAGTAAACTCAAATAAAAAAATCTTGTTCTTATATAATTTTAAAGTTTGTTCATTAAAATCAAAAAATGCTGATCCATATACTTCATTTTCTTGCAAATAGAAATTAAGTTTTTTGAGTGTTAACTCTTCTTCAATTTGATTATATAAAAATAAATTTTTTAAATCACGTCCTAGTGATTCTACAACATCATCATACTGAATATGAGTAATGATAAATTTTGTTAATGATTTAGGAAAATCTTTTAGTGAAAAGCGTAATTGATTATTATTAATTTTTTCATATGAAATAATTTTGCTAAAAGGATTGGTTCAATCAAAATTTTTAATCAAAAGACGTTTATTTGTCGCATTTAATATTTTTTGTATTGTTTGTGAATCAGAATCATGACTAAAACTAACTGTTGCAATTTGGTTTTGCTTATCTATTTCAATTTTTTCAAATTTAAAGTTAGTGTGATGATCTGTTAGTTGGTTTTGTTTTGTTAAGAGCGTTGCTTGATTAAAGGGTAGTTGTTTAAAATTATCTTCATTTTGAATAAAATTAAAATAATTATTAAATTCTTGGTTATTTTTCTGCATTGTTATTAAAGAAAGATCACTAGTTTTTTTTATGTTTTGCGGTGAAGATAATAATTGTTTTAATGCAAAAGGACGATAATCATTATTTATTAATTCATCATTAATATATTTTGTTTCAATAACACTTTGCTTCTTATTGATTTCTAAGTTTTGTGTATATTCTTTAGTTTGGTTATGCAAACTGAGTTGGTATTGAACAAAAAAATTATTTGACTTTTGTTCAATAATAACTTTTTTAATTATTATTTTAGCTTGCCCCAAGTCACCCACAACTTTAATTTTATTAATTTTAATTATTTGCTCATCTTCATTTTCAAAAATATCATATTTAAATTTAATGTTTTTTGCTATTATGTCTAAATTTTTAGTATCTTGATTCGTAATTGTAGAAGACGAAAATGTTTTTTTGTTATAAGAACAATTAGATAATGATATAGTTATTGAACCAAGAATTATTGTTGATACAACACCTATTGATAAGTTTTTTAATATTTTTTTTTGCATATAATGTCAATAATTTTATTTTTTTTAAAGGATTTTAATTATATATTAAAAAGAATAAAATTTTTTAAAAAGAATTGATAAATAAAGATAAAACGGCGTTTTGTTAAAATCAACACCGTTTTATCTTTAAATCTTTTTGTTAATTACTTTCTTTTCTTTAATCTTTGTTATTACTATTATTATAATTGGTGTTATGAATATTAAAAAGCAAATAATTGTTAAAACAATTACACCAATTAATGCTGATTCATAATGATTTGAGTATTCAAATGAATTATCAATTTTATTATATTGGTGTAATTTATCTAAAAAATTAAGATATTTCAAATAATCACCTTGATGATAATAATCAATTTCGATTCCAAAAATAATTGCAAAAGCCAAAAGAATGCAAAAAACTATTCCTAAAAAAGCTGACCCAATAATCTTCATAATTTTCCTTAATTTATTTTTAATTATTTTAACATAGATTAAACTAGATAAATTTAAAACATAATTAATTTATTTTAAGGGCTATTTATTTTTTAAAAAAAATAAAAAAAACAAGAAGAAAATTCCTCTTGTCTTTATTATTTTATCGATCTTTATAAGCGATTAAAATTGATTCGTTTAATTTAATTTTAGATTTTAATTGAAGTTTTTTCTTATCATCTTCTTCATTATTTAAATCATGTTTTAATTGATTTAACGAAGAATTAATTTCATCAATTTGAATTTCATCTAAAAATTTAAAATAGTCACTAAAAACACGAACTTGATGTTGTTCAATATAAACTATACCATCTAATAATAATAGTGGTTTACGTGGAGTATTTGGTAATTCACGAATATAAGCAACATGGTTTTCAATTGTTGACATCAAAGGGTTATGGTCAGGTAAAACGGTAATTCGTCCTTCTGATGTTTTTAATTCAACACTATACACATCTTTTTCTAAAATTTGTGCATAAGGTGTAACAATTTTTAATTTAGTTAAATTAGCCATTATTTATTTTTTGCTGCTTTTTCAAAAACGTCATCAATTTTACCAACATATAAGAAATGTTGTTCATGAATATCATCACATTCACCATCAAGAATTTTTCTAAATCCTTCAATAGTATCTTCAAGTTTAACTGATAATCCAGGTTGACCAGAGAATTTTTCAGCAACGTGGCTTGGCTGTGATAAGTAGTTACGTACTTTACGTGCGCGCATTACTAATTGACGATCTTCTTCGCTTAATTCATCCATTCCAAGAATTGCAATAATATCTTGTAATTCCATAAATCTTTGTAAAATTTCAATTACACGACGCGCAACACTGTAGTGTTTTACACCAACAACTAATGGATCTAATAAACGACTTGTTGATTGTAGTGGTGAAATTGCTGGATATAATCCCAATGAGGCAATAGCTCTATCTAAAACAACTTTAGCATCTAAGTGTGAAAAAGTTGTTGCTGGTGCGGGGTCAGTTAAGTCATCAGCTGGAACATAAACAGCTTGAACCGATGTAATTGATCCTTTTTTAGTTGATGTAATTCGTTCTTGTAATTGACCCATTTCAAAAGCAAGTGTTGGTTGATAACCAACTGCTGATGGCATTCGTCCAAGTAAAGCAGAAACTTCTGAACCAGCTTGTGTGAATCTAAAAATGTTATCGATAAATAATAAAACATCTTGACCAAAAGCATCACGGAATTCTTCAGCCATTGTTAAACCGCTTAATGCGACGCGCATACGTGCTCCTGGTGGTTCATTCATTTGTCCAAAGACAAGAGCTGTTTTATCTAAAACATCTGACTCAGCCATTTCATAGTATAAATCATTTCCTTCACGTGTTCTTTCTCCAACTCCAGCAAAAACTGATAAACCACCATGATTTTTAGCGATGTTATGAATTAATTCTTGCATTAAAACTGTTTTACCAACTCCAGCACCACCAAATAAACCGATCTTACCACCCTTTGCAAAAGGTACAAGTAAGTCGACAACTTTAATTCCCGTTTCTAGGATTTCAATTGATTCTGCTTGTTCGGCGAATGAAGGTGCAGGACGATGAATTGGCATACGTTTACCTGTTGGTGCAGGTTGTTCATCAATTGGATCACCAGTTACATTGAACATACGTCCAAGTACTTGTTTACCAACAGGCATTGTGATTGGTTTTTGAGTATTAATTACCTCTTGTCCACGTACTAGTCCATCAGTTGTATCCATTGCAATACATCTTGCAATATTATCACCAACTAATTGAGAAACCTCAAGTACTAATTTAGAACCATGATTGTTTAATTCTAAAGCAGTGTTAATTTCAGGCAATCATTCAGAGGGAAAACGCACGTCAACAACAGGGCCCAAAATTTGGTTAATTTTACCTTTTTTAACTTCTGTCATATTTTGCTCCTTATTGTTTATAGTTTCTAATCATCACTACCAGCAACAATTTCAGTAATTTCACGAGTAATTTTACCTTGGCGTAATGTATTGTAAATTAATTTATAATTTTCAGCCAATGCTTTGGCATTTTTTGTAGCTGCATCCATTGCATTGCGTCGTGATGCATTTTCAGAGATTTTTGATTCAATTAATGAACCATATAGAACTGTTGCAATATAAGTAGGTAGAATTTTTTTAACAAGCTCAAACTTGTTAGGTTTAAAAATAATATTATCTTTTGCTAATTCAATAGTTTCAGCAAGATGATCTTTAATTAAAGCTGTCTTATCGAATGGAAGAACATCTATAACACTTACTTCAAAAGTAATTGCGTTAATAAATTTAGTATATATAATACAAATTTTATTAACTTCACCTTGTGAATATAATTCAAGAGCCTCGCGCGCAATTTGTAAACTCATGTCAAATGTTAAATCATTATCATTTAAATTCAAATAAGTATTAACTTGATCAACACGACCTGATAAACGCATAAAGTCATGTCCTTTTTTTCCAATTGTAAATGTTATATCAGTTTCATTTACAATTTTAGAAATTAATTTATTAACATTATAGTTATAAGAACCCGCTAAACCCATTGTTGAATTAATAGTGATATATAAAGTTCGATCTTTCGCATTTGGAATTTTTAAAAATTCAACATCATCAACAACAGACAATAATAACCCAATAACATCATAGTAATCATGACAATATTCTTTAATTGCAGCTAAACGATCACGCTGACGTTTTAATTTTGCAGTTGCCACTAGTTTCATTGCATTCGTAATCTTAGCAGTTGTTTGAACTGAGTTAATTTTTCGTTTCATTGCATCTAAAGACATACTAGTTTGCCTCTAAATTTAAATTTATTTGTTGTAAAGAATTATTTAATTTTTTAGCCTTTTTTTGATTTTTAACTTCAAGTTTTGCAAGTTTATAAGCTTGTTTTTCTTCAGCACTTTTTTTAGGTGAAAATACAGTCGCTAGTTTAACAACAATAATTATAAAAGTAATCATTTGTGGCATCATAAATACAATAAATACTCAATTATATGCAATATAATTTGGATTTTGATATTGTATAATGGTTGATAATTTTGCAAGAGATTTTATTCCTTGAGCTGCTAATACAGGATTTTGTGAGTTAGCTAATTGTGAGAATAATTGATTATATTTGTAAAATCAACTATTTTCTCAATCACTTAATACTTTTGTTACAAATCCTCATGTTCCATTATGATTAATAAATTTACTATGCGCATGATAATGAAGAGCTTCATATAATTTTGGATCGTTATTTTTATTAGTATTATTAATTAATGACTCCATTGTTTTTCCATTAATCAATTGATCGTGAATTGTTTTATACAATTCTTGATAATTAGCGTGTTGATCATAACCTAATCATGACATTAAAGAATGATTATCACCACTAACATCAGCTACTTTACCAATAATTTGACCTTGATCATTAACTAATGCTCTTGCATTTTCAAAACCTTCATTTAATAAACCAACCATTGTAAACGCTATTGTTAATCCTACTATAAATATAACTAATGCTGCTATAAATCAAGGCGTAAAGACTTGTTTAATTTTTGGAAGTTTGCTGAATTCAGCAAAGCTTTTATTCATTTTAATTTGATTCATTATTTAAGATAAGTGAAACCACTTGGTCCTTTATTCATATTAAAACTTGCTAAATCTTTTGGCTTACGATATTTATAAACTTTAGAAGCTAATAAAGCATGTGCTTCTTTGTGTGAGTTAAAATATTCTAAATATTCTTTACGTGATGAAATTACTGAACTTGAACGATATTTATTTCAAGAAGTGTTTACTGAATTAACATCAATCTTTAATAAATCAAATTCACCAACAACTTTTTGTACTGGACTTGTGACATAAACTAATACTTTTTCAACTGGATATTTAGGTGTTACTTTAAAGAAAACAACACTTTTAGCATTATCAAACAATTGTTCTGCTTCATTTGATGAAATTGAAATCATAATTGTTTGTTTTTCAACTTCAGAAACAGCTGAATCTGAGAAGACTCAATTACGATTCTCGCGTTCATCTAGAATTGCTTCATCATGCTCAATTTCAGCAACTGGTACTAAGACTTCTAAATCATCATCTTTAGATTCATCATTATCTACCTCACCGCTTTGTTCAACGGTTTCATAAATTGAATATTCATGGTTTGTATCTTCTAAAACTTGTTGACCTTCGATTTTTTCTTGGTTTTCAGCTATTTCAAATTCATGATGTTCACAGATAATTTTTTCTGGCTCAACTTCTTCAAAAATATGATTTTCTTCCATTTTATTAGTTTCAAGAATTTCTTGTGGTTCAACGAAATCTTGAACTTTTTCTTCTATTTGAACAGCTTCAGATGTTTCTGTTGTAGGTTTTAAACTAACTTCTTCATTAATAAAATTAAAATCATTTTCACCATCAACAACAATTGATTCATTCTCATTAAAGAATTTATCAAAGGCTGTTTGTTCAGCTTCTTCACAAGGTTTAAAATCTTGTGAATTATTTAAGAAACTGTTAATTGCTTTTTGTATAGCACATCTAAATGCTGGGGTATTTTCTTTATCAAAAGCTTTCTTATCTTCAAGATCAGCACGTAGTTTTGTTCCTTTAAAGTAATTTAATAAGTACTCTTTAAAATCTTGAATACGTTCTACTGGAACTTGAGGAATAAGTTTTTCTTTAACGGTAAACAAAATGATCGCTTCATCAGTTTGATTATAAGGTTTATTAGGAGCTTGCTTAATAACAGCCATAACTGCTTTACCAAGTTTTAGAATACGTTTAGTTTCATCATCCAAATCACTACCGAATTGACTAAATGCTTCAAGTTCGCGATAGTTAGCTAATTCTAATTTTAATGATGCTCCAGTTTGTTTAACTGATTTAATTTGTGCAGCAGAACCTACCCGACTAACTGATTGCCCTGCATCAATTGCAGGACGTTGACCAGCATTAAATAATGATGTCATCATAAAAATTTGCCCATCAGTAATTGAAATTACATTTGTTGGAATATAAGCTGAAATATCACCAGCTTGTGTTTCAATAATTGGTAAAGCAGTAATAGATCCTGCCCCTAATTCATCTTTTAGTTTGCATGCACGTTCTAATAAACGACTGTGTAAGTAAAAAACATCTCCAGGATATGCTTCACGACCAGGCGGACGACGTAATAATAAGGATAAAGTACGATAAGCAATAGCATGTTTGCTTAGATCATCATAAACAATTAAAACATCTTTTCCTTGATGCATTCACTCTTCAGCAATTGTTACTCCTGTAAAGGGAGCAATATATTGCAAAGCAGGTAATTCTGAAGCATTAGCTGTAACAACAGTTGTATATTCCATTGCTCCATGAGCTTCAAGTTCACGAACAACATTTGCGATTGTTGAGTTTTTTTGACCAATTGCTACATAAACACAATTTACATTACGGCCTTTTTGATTAATAATAGCATCAATTGCAATTGTTGTTTTTCCAGTCTGTCGGTCACCAATAATTAATTCTCTTTGGCCCTTACCAATTGGGAACATTGCATCAATTGATAAAATTCCGGTTTCAAGTGGTTGATGAACAGATTTACGGTCCATAACACCAGGTGCAATTTTTTCAATTACACTAAATTTATTTGCAACAATATTACCTTTATTATCAACTGCCTTACCTAAAGCGTCAACAACACGACCTAACATTACATCCCCAACAGGAACTTCAACAATTCTTTTTGTACGATAAACACGATCGCCTTCTTTAATATTTGAATAATCACCTAAAAGAACAACACCAACAGCATCTTCTTCTAGGTTTAAAGCCATTCCTTCAACACCATTTTCAAAACGTACAATTTCATTTAACATGACATTATCAAGGCCATCAACCAATACAATTCCATCACCTAACGAAATTACATTGCCGACCTCTAAGGTTAAAGCTTTTTCAGAAAATTTCTTAATTTGTGATTTTATATCACTAATTAATGAATGATTTTTATTATCTGTCATTTTTACGCACCTTCTTTCTCTTTCGTATTGATGATTTGATTTAGTTTTGTTTTTAGAGAAGAATCAATTATTTCCAAGTTGATTTTTAATGATATACCACCAATTAATGATGGATCAACGATAAATTTAATGGGTCTTTCATCGCCAAAACGACTAATAACAGTTTTTTTAATTCTTTGTTTTTGTTCATCATTCAATTCAAAAGCAGTTTTAATTTCAATAAAACGAGTTTGATTAGAATATGCTTTTAAACGATTGGCGATAATTTTTGAATATTTTGCTAGTTTAGTATTACTTTGCATAATTACTTCCTAATTTACCTTTTTTACAATATTAAATAATTTAGTTTTTAAACTATTATCTAAAACTTGCGATTCAAAATTAATTGAAAGTCCTCCAATAATTGATTTATCAACAACAACAATTGGTTCTATTTTTTCACACACATATTTCTTTTTTAAAGATTGCAGAAGGCGATCAATTTGATTTTTTGTTAATTCATAAGCAGTTGTTATTAAAACTTTTTTAACACTTAAAGCTTCATTAACAAAATCCAAAGCTTTTAAAACTATTTTTTTAAGTAGTTTAACCGATTTAACATCAATTAGTAGATCAATTAAACTAATTAAATAAATATCAATCTCACCTTCTAGATTTTTAATCACAATTTGTTTTCTTTCATTACGATCACGAGCAGGATCACCAACAGCTTCAAAAAAAGCTGGAACCTCTTCAATGATAGAATTAATTGTTTCCAAATTATGTTTGTATAAATCTAATTTTTTTTCTTCTTTAGCAATTTCAAAAATTGAATAAGCGTATTTTTCAACTGGTTTTAATGAAGATTTCATAAATTATTTTTCATTATTTTCTAATTCTTTAATGAATTGATCAACTAGTTTATCATTATCTTCTCTAGACAATTCTTTTTTCATCAATGCTTCAGCTGCAATATAAGCAGCTTCACGAGCTTCAGATTGTAAACGTTCAAGATTTTCTCTACGTTCATTAGCAATGCTAATTTTAGCAGAACTAATAATTTTATCAGCAGCTTCACGTGCTTCTTTTTCTAATGATTCACGTAAAGTTAATGATTCACTTTTAGCTTTTGCAATAATTTCTAACGTTTGTGCTTGTGCTGCTAAATGTTCAGATTTAGCGTTTTCTAAATATTGTAAAGCTTCTTGTTTAGCATTTTCTGCATCTGTAATTTCTTTAGCAATATATTCACGTCTTTTATCTAGCATTTTATTTGTTGGTTTTCATACCAATCATAATACTAATGAAAAAACGACACACATTGCAATAACTTGAGCTATAAATACTCATACATTAGGGAATAGTGTATTAATAATCTCTGCTGGATTTAATTCTGGAATATCACCAGTACATGAAGTCAACAGTGGTACAACTATAATTGATAAAGAAAGAACGGATAATATAGCTACTAAATGCTTTTTATTTAATTTCATAACGTTACTCCCTTAATTAATTTTCATTATTTTTATTTAATAAATTTATTTAAAAAAAGAAAATACAATTAAGCAACGAAAATAATTAAGATAGAAACAATAAGTGCATAAATAGCAACAGCTTCAGCCAATGCTAAACCAACAATCATTGTACTTAAAATCTTTGGTTGTGCTTCTGGGTTACGAGCTACAGCTTGTACAGCATTAGCTGTAGAAAAACCTTGCATTAAACCAACTGCACCAGCTGCTAACATTGTAATTCCTGTACCAATATATTTACCTAAGTAAGCAATTCCTGCACCGTTTGCAAGTGAACCTACATTTTCAGAAGCAATTGCTGGTAAATTAGCTTCAATATGTGATGAAATAACATTTGTAATATCAATAAATGATGACATAAATATCTCCTAAATATTTTTTAATTTAATTAAACGTTAATAAGTTTTTTATAAAACTACTTGAGCCTCATTATTTTGAATTTGATTTTGAGTATCAAGTTCTGCTAATTTTTTAGCAGCAAGTTTTTCGGCTTTTTTATGCGCAAGATGAGCGTGGTGTTCTTCACCTTCTTTGATTTCAAGACCTCAGTTAATACTTGTTAATAATGTAAATACAAAAGCTTGAATTGTTCCTGCAAAACAAGTAAAATAACCAATTAAGATTGGTAAAATTACTACTGCAGCAATTAATGATACACCAACTCAAGGAACTACGTTTGTAAAACCTGCAAATGCTCAATATGTTATGTTTAATAAAATTGCAGCAGCAGAGATATTACCTCATAAACGGAATGTTAATGAAATAATAGGGGTTAATTTAGAAACGATTTCCAAAGGGTTTATCATAATTGGAAAACCTTTAATTTTAAAAGCAAATTTTAAAAAGAATGAAGCTTTTTGGTATTTTAAGGCAACAATTTGACCACCGATCACGGTTCCAAGAGCCATTGCTAATGGAACGGCAGATGCAGTTGTTGCTTCTTTGAAACCAAATAAAGAAACTAAGTTAGCTGTAAAAATATAACAAAATGTAAATAGTACATAAGGTGCTAATTTAACATGTTTAGCGCCCATTGTATCAACAACTAGTCCTCTAAAATAATCAACAATCATTCCAATAATAATTACAAAACGGTTTGGCACATCATGTACAGTTAACTTTTTAACCATACTAAAATAAATCAACGAAACTGTTCCAATAATTAAAGTTACCATAATGATTGCAGCAATATGTGGAAGTGCAATCATTATATCAAGCGGGCTATAATTTTCCACTGTTTCACCTCCAATGAATAATGAATTATTTTTTTTCTCCTTTATCTTTTTTAATTCCTAAATAAATTTGCATCCCCATTTGGATAAAAAACATAAAAAATGTTAAAACAAATAATGAGATGGTCTCAAAAATAGATTGATAAAGGTTAACAAGATTACCAATGATCAAAGGCACTAATATTAGAAAATTAAATAAAATGTAAATAGTGCCAAAAGCAAAAAAATTTGCTGGTAATCGACGATATTTTTTGGGATTGACACCTAAATTATTCATTTTTAAACGCTTATTTAATAATTTAGAAATAATGAAAATTCGTAAAAGTGTAATTCCAAATCCAAAAGGAATAACTAACAAGTAACTATAAAAAAAAGATCAACTAGCTTCTAATAAAATACCCAAAAAAACAAATACCACAAACGATACTGATAGAAAGGGTAAAAAAATTATTAGATAACTATTGATCATTCTTTTAAGATTCACATTTAATCATTCTCTTTTAAAAAATTAGCCAAAACAAGTTGGCTTGCTTATATTATAAATAAAAAATGATGTTTTTATATATTTTAATTACACGTTATTACTTAAAAAAACGAGTTTGTATTATAACTATAATAAATTAGTGCTTTTTATTAAAGAATAATCACTAATAAATATTAAATGAATTATTAAAACAAAAGATAAGGAATACAAGAAGGTATGGATGAACAAATTTTTATTCAATTATTAGAAAACAATCTAACAAATTCAAAATCATATGTGACTCAACCAATAATATTAGAATTTAAAACGGATGTTTTTTTGATTATAAATCAAATTTTAAAACAATATAAAAAAGGTAAAATTATCAAAAATAATACAAATGAAATTATTTTAGAAATTGATAATAAATTAATTTCTATAACAAATTTAGATATCAATAAATTTCATTCGTATCAAATTGTTAATATTAATGAAAATAAAAATATTTATAACGAAAGGAATAGTTTAATTTTATTTGATTTAGTAAATTATATCATTGAAAAAAATCAAAATTCTATCAGACACATTAATTTTTGAACGATTCAAATGAGTGTAGTTAATTGAATTTATTTTATTACTTATAATTTTAACAATTCATATTTAATTGATCCAAATTGAAAAAAATATGTTTTTAAAATTAAAAAAGATGAATCTAAAGGTGTAATTAGTACCAATTTATTACATAATTATGGTTTTGTGGATTTTGTTGTACAGTCTAAATCACAGAATTTTTTAAAAGCTTATCGTGTTCAAGATGAAATTCTAAAATCTGTTTTAAATTTAGGCGATAACTTAAATAATGAATTTTTAGAAGAAATCATGAGAAAATATGATACATACAAAATAATTGATAGAGATCATAAATATTTAGTGATCAATATTGAATAAATTATAATATATAAAATATAAATAAAATCTTATTAACTTAAAATTTAAATTAGGAGATTATATGATTTTTTTAAAGAAAATAGAAGCACATGGGTTTAAATCATTTGGGGAGCCAGTTGTTATTGAATTTAAACACCCAATGACTGGAATTATTGGTGCAAATGGAACAGGAAAATCAAACATTATTGATGCTCTTAAATGAGTAATTGGTGATCAATCATTAAAAAGCATGCGTGCTCATAAAAATGATTTATTGTTTAGTGGTGGAAGATATGCACCGAAAGCCCATATTGCACGTGTTAATTTATATTTTAATAATGTTAATAATGTTTTATATACACAACATAAAGAAATTAAAATTTCACGAGTGTTGAATATTAAAACAAACGAAAATACTTATTATATTAATGATGAAGTTGCTCTTTTAAAAGATATAACAGATATGTTTTTAGATAGTGGATTATCAAAAGGTTCTTTAGGAATTATTAGTCAAGGTGCAGTGTCATGATTTGCTGAAGCAAAACCGAATGAACGACGAAAAATGTTTGAAGAGGCCTCTGGAATCGGGCGCTATTCAAAGCGAAAGCAAGAAGCGTTAAATTCACTAAGTCGTGCAAACGAAAATTTAGCTCGTTTAAATGACATAGTTGCTAATTTAAAAAAAGAATTGGCGAAATTACAAAAGCAAGCAATGCGTTTTAATGAATATAAACAAATTAAGGACGAATTAACAAAATTAGATTTAGTGATTATAGTACGTGATATCATTCACTGACAAAAACAATTGAATGATATTCAAGAAAATTTAAAAGAATATAAAAAAGAATATAATAAACAAAAACCATTAATTCAAAGTAAAAAAAATGAAATTGAAAGTTTACGAGTAATTTCAGATAGATATGCAAAAGAATACACTAATTTAGAATTTGAATATAAAAAATTAGAAGATACAATAAACGATTTGAATCTTAAATTATCTCTAACTAAAAATACACTTGAAACAACCGCTTTTAATGCAACAAATACACAAGAAAGGATTGATGCTTTTGCTACTTTAATTAAAACAACGGAAATAGAATTAAATAATAATAAAAAAAATATTAATGAAAAAAAAGATCAACTTAAAGATATTCAAAAACAAAAATTAGAACTTGAATCTCTTAAAACAAAACAAAAAAATAATTTGGATACCATCATTTGAGAAATTGCTAGTAAGAATTTAAAAAAAGAAATGTTAGAAGAGCAATTTAAACGCCAAACTGAGAATGAACGTGGTGTAAAAACAATTATTGATTCACAAAAAGCATTAGTTGGTGTTTATGGAACAGTTGCGCAGAATATTTCTACAAAAACAGAACACGAATTAGCAATAAGTACAGCTTTAGCTAAAGGAATCAATAATATTATTGTTAAAAACAATCAAGCAGCTGAAAATTGTATTAATTTTTTAAAGAAAAATAATGCAGGTGTTGCTACTTTTTTACCGATTGAAAATTTGCAGGCTAAAAGCATTAAACCAGAATTTTATGATGTATTAAAAGAATTAGAGGGGTTTGTAGGAGTTGCTAATGAACTAGTAAAAATTAATGTAAATTATAAAATTTCTATTGATTATCTATTAGGAAATACAATTATTGCTAATAATTTAAAAAACGCTATCATTCTAAGTGAATACACATATAAAAATTATAAAGTAGTCTCATTAGAAGGTGACATTGTTTTTGGTGGTGGTGCCATTTCTGGTGGAAGTAATAGTAAAAAAAATATTATTTTAAATCTAGAAGAAAAAATTAAAGAATTAGATTTAAAAATAAAAAAATTAAATGAAGAACAAATTGAATTAAATAATTTACATAACCAAACAAAAAAGCAATGACAAGAATATTATAATAGTGAACAAATTATAAATGGTATTTTAAATAATTTAATTACTACTAACAATAGTCTTTCTGATAATTTAAATAATTATAAAAGTCAATATGAGGCTTTAAGCGCTACAAAATTGTCTAAAGAAGAGATTGAATCAAAGATAAGTGATAATTGAAATGAATATAATAAAAAAAAGAATGAATTAATTAATTTAAAACAAAAAATCCAAACTTTAAAAGCAAATTTAAACACTGCTAATCTTAATAAGAATGAATTAGAAATAACATATAATAAGGAGCTTGAAGATTTTTATAATCTTGATAAAAATATTACTTCGTGTGAAAGTAATATAGATAATCTAAATTATTCGATTCAAAATGTACAAACAAGATTAGTAGATAATTATAAATTAAGTGTTGAGAATGCTATTGAAACATATCAAAATCAAGAAATTGATATGAGTGAATCAGCAGCACGTTTAAGAATCGAAAGTTTAAAAATTGACCTTGAAAAATATAATGATATTAATTTAAATGCGTTAGAGGAATACGAAGAAAAACAAGCAGAATATGATTCTAATTTTTTAGAGTATGAAAATTGCAAGCAAGCGGTTGATCAAATTAGTAGTTCAATTCAAGAATTAGATAAAAAAGCTAAAGCTGATTTTATGCGTGTTATTCGTGAAACAAATAAATTAATGCCAGAGATTTTTGAATATTTATTTGGTGGAGGATCTTGTGCAATTAGTCTTAATGATCCTAATAATATTTTAGAATCAGGTGTTGAGATAATTGCTAATCCACCAGGTAAACGTGATGTTCCATTATCTGCACTTTCAGGTGGGGAAAAAACAATGGTAGTATTGGCAGTTTTATTTGCTTTATTAAAAATTGCAAAATTTCCATTAGTTATTTTAGATGAAGCTGAAGCAGCTTTAGATGCAAGTAATGTTGCAAAATTCGGTGCCATTATCGCTAAGTATAGTGATGAAACACAATTTTTAGTTATTACTCACCGCGAAGGAACAATGAAAAGTTGTGGTTATCTTTTAGGTACAACAATGATGAATAACGGAGTAACAACATTACTAGAAGTTGATTTAAATGATACAAAAATAAAATATGAAAGTGAATTATAAAAATGGGTTTTTTTAAAAAAATATTCAATAAAATTCTAGGAAAAAAAGACACAGAACAAATATCACAAGAGATTTCACAAAAAAATGAAGAAAATCGTATTTTAAAATTAGAGGATAATTCGATTAATAAATTTAATGAAGGATTAAGAAAATCATCTTCTGCTTTAACTGATGCTATTAACGAATTAGCAGTTAAATATATAGATATTGATGAACGATGATATGAACATTTAGAAGAAGTTTTAATTAGTTATGATGTAGGCTATGTTGCAACTAATAAAATTATTGAATCAATTAGAAATGAAATGATTTACCAAAAAGTTAATGATCCTGATTTAATAAAGTCTATTATTATTGATAAAATTTTTATTTATTATATTCAAGATACAGAAATTAATACAGCAATTAATTTAAAAGAAAATCAAACTAATGTAGTTTTGGTGGTTGGGGTTAATGGCGTTGGTAAAACAACATCAATCGCAAAAATCACTAAAAAATTCATTGATGAAAATAAAAAAGTTCTCTTAGTAGCTGGAGATACATTTAGAGCAGGTGCTGTTGAACAATTAAAAGTTTGAGCACAACGCTTAGGTGTTGACATTGAATTACCTAATAAAGAGGGTCAAGATCCTGCTTCTGTCATATACGCTGGAGTTAAAAAAGGTTATGAACAAAAATATGATTTAGTAATTTGTGATACATCAGGTAGATTACAAAATAAAATTAATTTGATGAATGAATTAAAAAAAATTCATGATGTAATTCATAAATTTGATGAATACGCTCCGCACGAAACTTTATTAGTTTTAGATGCCACTCAAGGCCAATCAGGAATTAATCAAGCAAAAGCATTTAACGAAGTAACGAAAATTTCAGGGATTATTTTAACTAAAATGGATTCTACAAGTCGTGGGGGAATTGTATTAGCAATCAAAGATGCTTTTAATATACCTGTGAAATTGATTGGTTTAGGCGAAAAATTAGAGGATTTAAGTGTATTTGATTTAGAAATGTATGTTGATAGCATAGTTTTAGGAATGAAATTAGATGTTAAATAAGAATAAACGTTGATATTTGATTGCTTTATATGATATTTATCAAGGATTATTAACAACAAAACAATGTGAATATTTTAATTTACATTATTTTAAAGATTTGTCGTTTTCTGAAATTGCTGAACTAAAAGAAATTTCAAAAAGCGCTATTAGTGATTGTTTAAACAAAGTGTGTGACCAATTATTAAAATATGAACAAGCACTTTTAATTTATGAAAAAAACAAAAAGCGTAATGATTTATATACATTAATCAACGATTCTGAATTAGTTAAAAAATTAAAAGATATATAAATTTCTTATTAAAATAAAAAGAAAGAAACGGTTATTAAATTAACAACGTTTCTTTCTTTTTTTAATTTTGAATGTACATAAATCTTATTTAAAAGGATTTATATTACTTATAGATATCCTCATATTTTTCTGGATATAAATAAGCTAATTGTTTAATTTGTTGGATAATCGAACTATTGTTAGCTCTATTTTCCCGATCACCAAAAAACCCACTAATTTGACTATCTATTAAACTTCAACCTATAGTTTCTCACGTTGGATGACCATTTTTACTTGGAGTTCTACCAGTTGCAATTGATCCAACGAATCGTCCTTGTTCATCATATAAACTAGTTCCAGATGAACCACCCGCAGCATCTATATAGTCAGTACTAATTGTTGTATATGGTATATCTGATTTATATAAAACGCCCTTACCACCTGTGACAAAATTAGTATTTACAGAATCGATTTTTTGTATATTATATTCACGATAACGTAGTTCGCCAACCCCCATTGAATTTTGGTTAGTATTTGTATAACGAGGGAAAGTGCCAATATACCAATCAACATCTTGGTTGCTTCTAACATATCTTGTTTGTGGACTAACCTCTAATGGTTTTATATTTTTTAAACTTAAAAAATATTTTATAACACTAGTTTCTTCTGGAGACAAAGTTTTATTATTGTAAATAAACGGTTTATTTAAATCTTGATTTTTAAAAGCTTCAAAAATTGGTTTTAGATCGATACTAACTACTGACCAATCTAATTCACTTATTTTATCATCATATCTACCTAAAGCATTGTAGTGTTTATATATATTATTTTTTTGTAAACAAAAATCCATTACTTTTTCAAATTTTAATTTAAAAGGAAAAGCAAAATATTGAAGTTGATTTTTTCCTGGAGTATCTTGGTTATTAACACTACTATAATTATAATATGGTTGATATTTATCTTTATTTAAATCATTTTGACTAATTGTTTGAGGAATTACAACTGTATAATCTGCAAAATTCGAATATGTGTATTTTTCATTTCCATCATCATCGTAGATAGTTTTTTTATTTAAATTAATACTTTCATATTCCATTTTTCCAGCCCAATTTATTTGGGGATTATATCAACTACCACCACTACCAATAACATGATGATCAGTTAACATATAATAACGATAATCATTTGGATCATTTGGTTTTACCTTACCTAAAAAATTTCAAGTACCATCACTTGTTAAACTAAAAGTAATCGTACGCTCACGAACTTGTTTTATTGCCGCTTTTGACATTTCATTTTCATTGTCTTTTAAAATTCTTGCGGTATAAGATTTCTTTTTAAATTCCTCTAATTTTTTATCGATAATAACTTCATTATTATTTGTTAATCAATTAACAATAAAATCATATCTTCCAATAAAATATGACGGTTGATTTGAATTATTGTGTTTTCAAACGTAATCATAAAGCTTAATATTATCATTGCGTGGCTTAACTTCAACTTTTATCTTTGAACCATTTAAAAAGACATTAAATTTTAATTGTGATTTAATTTGTTGATAGATGTGATTTTCATAAGCTTCAGAACCTAAAGCATAATTTAAACTAAATTCAACATCTTCTAAAATGTGTTGTTTTTTAATTACTTTATATGAAATAGGAATTATCACATGCGATCTTCGCATGTTTTTATCTACAAGTTGTGATTCATAAATTAGATTAGGATCTAATTCTAGATCTACATTAAAGACAATATCTTCATCTTTAAAATTATTTAAATTATCAAAATCATTCAAATCTTTTTCTAAGAAGAAACCAGCAGATTCATCCTTTTTTAATCCTTCATCTTTAATTGCTAAATGTGCAATTAAAGAATTTAGATGATATGACTCTTTATTATTTTTAATTAAATTAAAATCTTTATTACTTGTTATTGTGTTATATAAATAAAAACCATTAGTAGCATTATGCTTATAAATTGCATATTCTCTTTCATGCAAAGTTAATGATATTAAATTGCGCAAAGAATAAGGGATATTTTTTTGATCAGTATTATTTTTTCATAAGTGATTAAAATCTATTTTTGATAAATTAAATTCATCTTTTATTAATGAATCATCAAAAAACTCATTAATAATATTTTGCTTTGGATAAACTTTATGTCAAACACTAAAATTTGTATTATTAGCATTTTGAATATTAACATGATCATTATAAGGTAACAAGCTGTTTTTATTAACTATTTTTATACTTTCAAGTTTATAATTTAATCCATCGAAAACATTAACAATTTTAAATTGTGCTAGATTTTTAAAATCAACATTTATATATTTATTTAATGGTATATATTTATTTAAAATATTATTTTTAGTGTCGATTTCATATTTAAAAACAAAAGTTTTATTTTTTAGGATTTTAAAATCATCATTATTAAAATTAAAACAAGCAGAACCGTATAGTTGATTTTCTTTTATATCAAAGTAGTATTTAAGTGATTTTAGTAAATATTCCTTGTCGTTTTGATAGTATTCAAAACTATTTTCTTTAATTTTACCAAGCGAAGTTATATTATCATCAAAACGAATATGAGTAATAATAAATGTTTTCAAATCTTTTGGAAAATCATGTAAATCAAATATCATTTTATTATTATCTTTTTTTTCATAACTAACAATTTTAGATCAGGGATTATTATAATCATATGATTTAATTAACAATTGTTTATTATTTGCTTCTAAAAGTTTAGTATTATCATTAGAACTAGACGAATCTAATTCAATGATTGCTTTTTTATTACTACTATCGATGTTAATATGTTTGATTTTAAATTTAATTTCTTGCTTTGAATTTTTATTAATATCATTAAAAGAATAGTTTTGAAAATCTTTGTCTTTAAGGTTTGCTTGATCACTAATAAAACTAAAATATTTTTGTAATTCTAGCTCATTTTGATTTAAGATGTCTTGATTAAGATTCTCAACTTCTTGTATATTAATGTTTTGCTGTTCAAATGCATCTGGCAACACTTTATCATTAAAATTATTTTTATCATTAAGTTCAATTTTAGTGTTATAAGGACTATCAATATATTTTTTGTCATTACTATTCAAGTCTTTATGATTATGCATATTTGTTTTAATACCTGGTATGATAAAACTGCTTTCATAATTAGATAACTTTTCAAAAATATTTGGACTAACATTAGGTGGATTAGTATCTAAAAATCTAAGATTTACAATTCGATATTTATTATTTAATTTTAAATTATCTAATTTAAATTCTACATTAAAATTTTTATCAATTCTACTAATTATTTCGTTATTAGGCACTAAATTACTACTTAAATTTTCAAATTCTAATGCTATTTTTTCATTTACTCTTACATCAGAATTTTTAGGAATGCTAAAAGATATTAATGCATTTTGATTAAAAACTTTTATTTTAAAATTATTGGTTTTAATAGGTATTGGTTTTATTGAAAAAATAGCATTATTTAGGTTGGTTTTCAAATAAATGTCTGTTCTTTTTTGATTATTAAAAGTTAAATTTTGAATTTGATATTTACGATTAGGGGTTAAATTAGAAAAAATAAAATCATAATTTTGTTCATCATTAATACTTAAAGGATCTGATGTAAATTTGTGACCATTATTATCTTTATAAGTTAAGGTTATAGTAGTATTTGGTGATTTAAAATTAAATTTTTTTAATTTTAAATTAATAATTGTTTTATTTTGATTAATAAATTTAGGTTTACTAATATTTTCTATATTAATTATATTTGCATCAAAATCGTCTAAGACATTTTCTTTATTTGTTTTTGGTGCTTCTTGGTTTGTTTTTGGTGCTTCTTGGTTCTTGCACATTATTAAAAAAGAAGCAAGGGGGAGCAAAAATAATACTCCACCGAATTTTCATCATTTTCTGTTTTTTATTCTTTTCATATTTTTATTTTATCTGAAATATTTTAAAATATTTTACTATTATATTTAATTGCATCCATAATTAAAAATATTGTAGTAAAAATAATGTTTATCATTCTATATAGAGAATAAATATTTTGTGTAATTAAAAATATTTTTAAAAAATATTGCTTTTTAAATTTGTCTGTTGTATAATAATATGGTACATTGGAAGTATAGCTCAGTTGGTTAGAGCACACCCCTGATAAGGGTGAGGTCGGTGGTTCGAGCCCACTTATTTCCACCATTTGGGGACGTAGCTCAATTGATAGAGCACCTGATTTGCACTCAGGAGGTCGTGGGTTTGACCCCCATCGTCTCCACCATTATAATTATAAAAACCAAACCAAAACGGTTTGGTTTTTTCTTCTCTAAATAAAATTAAAAGCCCATAATTAATTAATAATTACAGGTTTTTTGGTTTTTATTTTTTATTTACTACATTATCTAATTCAATAAAATTTTTAATAATGTCTCTTCCTTTATAATCTATTCCAACAATTTGTTTAGCAATTGCTGTAGCTACTTTTATAGCAGATTCTTTTGTCTTTTTAATTTTTTCATCATTGGCTTGTTTATTATCATTAAAGTCGATTGGATTTTGCTCTAGATCAATTGGAAAATCAGCATTAACATTGAATTGTTTATTTAAATATGGTAATAATTGTTGGTTAAAGGCGATATTAGCCATATGATACTTAGCATTTTTGTTTAATTTTTTTACAAAAGAAACAATAGATGAATTCATTAATTGTGATTTTTTAATTTCAACAACTGAAATTTTTGTTTTATCTGTTGGTGCAGCAAGATTGTTTTCTACTTTTTGATCTTTATTATATTCGTAGAAAGTTAAACGTAAAATTGTTGGATCAGATATATCAATTAATTTATTAGTATTAGTGTCTAACTTTTTAAATGCAATATTTTTTAGTTTTTCAGATATATCAAAACCTAATCCAATTGAAAATTTAGCTTCTACTTTATCATTAGAATTTTTAATATCATTGGTTGATAGATGTCGAAATGAAATATGATTTTGTTCAATATTATTAACATTATCATTATTTAATGCAATATTTGCAATTTTAACATTAGTCAATTTAACATCATAATCACGATTATTAAACTTTATGTATATGGTTTTATGGAGCGACAAAGTATGATAAACAAATTTCTACAAAGTATTAAAAAACGTAATTTATCAAAAAATACATATTTAACTTATGAATCACATTTAAGAAAGTTTAAGGTACAAGAACAAAGTTTTAAACAGATTATTAAGCAATTATTAAATAACAATTACAAACCAAAAAGTCTAAAATTAGCTAAGTCAATTTTAAGTGAGTATTGAAAATTTAGCAAACAATACCAGTATGTTCAAGAATTAGAACAAATAAAATTAAGTAAAGAACAAAAAAGTTATAAAAAAGTTTTTAGTTTAGATGATATTAAAAAATGTTTAAAAATTAATAAACAAGATACTAAACAGATTAAGTTTTATAAAACGATTTTATTATTCTATGCCACAACTGGAATTAGAGCAATGGAAATCTATCAACTTAAACAAATAAACAATGACTTATTCGTAATCAGTGGTAAAGGTAATAAGAACAGACAAATTTGCTATATTGAACATCTGTGAAATAACATTAAAAACAAAAAACTACCATCAACTAAAACATTACGTAAATATCTAAAAGTATTTTTTGATAATAAAAACGTTTCATTACATACGCTAAGACGTAGTTTTATTACAAACTTTTTAACTAGCAATGAAAATTATAAACGTGGAGATATGCTAAAGATTGTACAGGATTTAGTTGGTCATGAAAATATCCAAACAACACTACAATATGTACAAATCACAAAAGAACAAGTGGACGACGTTTATAAAGAATTTTTTAAGGAATTTGAAAATGATAAATAATATTTTTAATAAAAAGTTGTTAGTTTACAACGAAAAAGATCAACGTTGGTATAACAAATTTGATAGAACTAAACCTTTATTTTCACCAAGTAGACTATTAAGTTTTAAAAATTACATTTTTGAAAAAATACCAGCAAAAAATCTAGAATATCCAATTGAGTTTGGAACTAGTTTAATGCAAATCATTCAATACTATTTTGAAAATCAATCAAAATTAGATGTCATTTTAAATAGTATTAATGAAGAAAAATTAAAAGAAGTCTTTTTATTGTTTTTAGACTATTTAAAAACAAATGATTATTCACTAATCGCTGTTGAATTACATTTATTTAGTCCAAAACTAAACCATTACTGACATGGTTTTGCTGATGTTGTTGTTAAAACCAAAGATGATAAGTATAAAGTACTTGAAATCAAAACACACAACGAAATCCAATCAGTGCCATCTTGAAAGTTTCAAGCTGGTGTATATGATTACATCATCAGCCAAGAATTAAGCAATTACGAACCATCAGCTATACTAGCATTTAGTCGTAAACAAGCAAAGTTAAGCGTTTATGAAAATGATTCATTAGATTTAAGTTTTATTAATAAAATCAATGAACTATATTCTTAATAAAAACAAAAAAGTAAGCTTAACTGAACTAATGAATCAACAACCACATTGATACTTAGAAGAACTACAAGAACATTTAATTAAATATCTTGATACAAACGGACTAAACGACACTAAAAAACTAATTAAACAAACAATTAAAGAATGGAAAAAATAATATGGATAAAATTAAAAATTATCTAGAAAAATTACAAGCGTTTTATAAGTTATATTCTCTTAATTTAACTCAAGGAACAGAAATTTTAATTATTCAAATCATTGATAAAGGTAATTTAATTTTTGATTCCATTGAAAAAGGTAAAACATTTAACGAAAGTAAAATAATAGAACCACTTTTATTCATTTTTAAAAAAATAAATACAATTTTAGCTATCTTAAAATTAACTTTTACTAAAGATGATTTAATTGGAATTCTACAATCATTAGAAGAGCATATATCAGAATTTATTATTGCAAATTATAAATATGATTTTGAAATCGAAGAAGATGATGAAGATTAATAACAATGGAAAAAATAGTTGGCTTTATTGTGAACATAATTAATGTTCAAAAACAAATGATAAAAGACTTAAAAGAACTAAAAAATCATAAAGACATCATGGTAAAAGACCATGAAAAATTAAATGATATGTTAAATAGTATGATAGATGAAGCAGTTAAAAATTACAAAATTGCATTAAAAAACATAACAGAAATTCTAAACGTTCATTTAAACTGTGTTTTATATAATCACAATCTTAAATGTGGCGTAAAAGATGAAAAATTATTATTACCACTATTTAAAAGAAAGAAAGCGAGTTAATAACTTATGGCAATGCTTTGTATGTGTGATGAATGCCAAGCAATTATTAATTATTATGTTGTACAAATTGAACAATTGTGTAACGAAATTGTAAAAACAAGAAAAAGAGTTGAACCATATAAAAAATTGGGTAAATTGTTTTTTGAAATTATTCAAAATGATTTATTTGAAGCAATAGCTGAAGAAGTGTTTAGAATAGATGAAAAACCAAAACGAAAAACAAATAAAAGAGTATGTAAATAAATTTAAAGATTTGCGTAATCAACTAACAAAAAACAATGACGTTAAAAAAGTTTGCGATTTAATTAATGATTTGTTTTTTGAAGTATATAAAAACAATTTAATAGATGAAGTTATCAAGGAGATTAACAAACATGACTAATTGAGATAAAGAACATGCATTAAGTGAAGAACTAAGACAATTAAAAGATGAGTTCGAAGATTTAATGGATGATTTAGCAAGAATTAATAAAGATTTAGATTTTAATATCTGAGAACGTAAAGACTTAGAATATCGTAAAGCAAGTTATGAGACAGATATCGAAAGTATTAGATTACGAATTAACCAAAAACAAAATGAAATGATGGAGATAATAAAAAATGACAAATAGTATTAAACGTGAAATATTAAGTGCTTTAAATGATGAAACAAAAAAAGTAATTAGCAAATTACAACAAACAAATAATTTAATTAAACAATTAAGCGATGAAAAAAAGACATTAGAACAATATTTAAAAGATAATGTTAACAATCATGAAATCGTTGATTTAGACAGTGATTTAAAAATTAAAATCTATGATTTAACATCTAAGGATAGTATTGTTTTAGATTATGACAAAATCATTACTGATTATAAAGTTGATACAAAAAAATATGAAACGATTAAAAAAGGAACAACATCAACACGAATAACATGAAGTGGAGCAGATAAATAATGGTTAAAGACGATAAATTAGTGCCAAGCATTAGATTATTAACACCAAATGAACTAAGCGAACAATGAGCTAATCCAAATAGCGAAATTAATCAAATCACACGTGCTGTTTTGACCATTCAAGGAATTGATTTAAAAGCCATTGATTTAAACCAAGCAGCACAAATTATTTATTTTTGCCAAGCAAATAATTTAAATCCACTAAACAAAGAAGTTTATTTAATTCAAATGGGCAATCGTTTAGCACCAATTGTTGGTATTCATACTATGACAGAACGTGCTTATAGAACTGAACGATTAGTTGGTATTGTACAAAGTTATAACGATGTTAACAAAAGTGCTAAGACAATACTAACAATCAGATCGCCAGGTTTAAAAGGTCTTGGAACTGTTGAAGCTGAAGTATTTTTAAGCGAATACAGCACGAATAAAAACTTGTGATTAACAAAACCTATTACCATGTTAAAAAAGGTGTCTTTGGCCCACGCATTGCGTTTAAGTGGCTTATTAGCGTTTAAAGGTGATACGCCTTATATTTATGAAGAAATGCAACAAGGCGAAGCAGTGCCAAATAAAAAAATGTTTACACCACCAGTTGCTGAAGTAATTGAACCAGCAGTTGAAAATATAAAGAAAGTAGATTTTAATGAGTTCTAATAACTTTGATAATAATATGCCACGTATTGAATTTAAAAAAATGCCACTAACTTTTATTAGATTAAATACGAAAAAAAGTAATAACGCACCCTTTGTTTCAGGTAGTTTTTGATTAGCAGATAAAGTAAATGCGAACGTTTATTTTAGTGAATATTTAATGGAACGTATTGTTCCATTTGAAAACAATAAAACTAATATCGTTATCTTAGAAAATGTTTCATGTTATATAAATTTTAACTGAGAAGAAAAACGAATTATTATGCGTGTTGTTTCATTTGATAATATTTTTTACAAGCATCAATTAAGCGAACAACAAACTATTGATTTTGTGAGTGCTGAAAAAGAATATAACTATCAAACACAAACAGTTAATTCTTATAGTAATAGTGATGATGAAATTGAAATTATTAACTGATTAGAAAGTGATGAACAAAAAGCATATTTTGATAGTCAGTTAATGTTTATTGATTATAAAAACCAAGGCTTTTTTAAAAATAAAACAATGCCAGATAAACTAAAAAATAACGCTAATGCTTTAGAACTTTATAAACAAATTATTAAATTAGATTTAGAAGCATTTAGTGGTTTATACAACGGCTTAAAACCATTTGAAGCAGCAAATAAATTAATTGAAAAAGGCTATATAACACTTAACGATTTCTTTAAATATGCGATCTAAACAATGACATTTGAGCACACAAGATGCTAATTTAGCAAATAAAAAATATGATTATATTTACTTTAGGGGTGTTAATCAACTTTATTTAAAACGTCTTAAAAAAAGTAGTAATAAGTTTTTTGTTTATAAAAAATGTAAAATCGTACCACGTATGATATCAAAACATTTAAATAACGACCATTTAATTAACGTTAATGATTATGTACCACTAACCAAAGAATTTATTAAAAAAAGCGTTGAAAAAGAAGTTAAACATTATCTAAGGTTTATATCTTATGAAAACAAAACTAAACAAATGCCTGAACTAATTAATTTTTTAGAAAAATCACTTGAAGATAAGTTATTTATTGTCGCAAAAGATTATGATCCTTTATTTTCTGTGTTTTTACGTTATTACATTCAAAGTTTAATAGAAAAATATATTAATGAATTATGAATAAATGAGTGATAGGAATCGATTTATCAACAACAATAACAGGAGTTGCTATTTTAAGAAATGAGCAGATAATTAAAACCTTTGCTGTTGCTTTTGATAACTTTAATGAAAAGAATTTATATAACAACGTTATAAGACTTATTAAAGAAATTAATTTAAATGTTTGATTATTAGCTGATGATGATTATTATATTGGTATTGAAGTTGCTAACTTTTCAAATCCAAAACTAACACAGCGTTTTTCAATTTATGCTGGTATGATTATTGCGTTAATGTCACAAGTTTTAAGAGATTTTAACGCTGAATTTAAAATGTTTAATGCGAATGCTTGACAGTTAAAAATACCACAAATACAATATAACACATTGCGTAAAGATCGTAAATTAATTACTAAAAATTTAATGATTAAAACATTTAATATTAAAAGTAATTTAAACGAAGACGAATACGACGCCTTAGCGATTGCTTATTTTTATGATAGTATTAATTCAACGCTAGAACAAGAAGAGATAACTAAGGCTAAAAAAATAGTTAAAATGAATAAAGTTAAACAGCAGTTATCAATTAGTAAAAAGATTAATAAACTGCTTGAAAAGAAAAGCAAACTAAAAAAAGCAACTGCGATATCAAAAGTTGATGAACAAATAGAAGAATTAAAGAAACTAAATAAATAATGTTATAATATTTATCTCGTAGGTAGTCATACTATCATTTGCTAAATAGTATAACTAGAATAAAAAAAGGTGTTAGTTGAATCTAGCACTTTTTTATTTTAATAATTTAAAACAAATTATATATTATGTTATAATATATACACTAGTACACGTTGATATAACGCTTACGTGTTAAAATAAAATAGCGTTATTCCTTCTCTTCTTTTTTACAAAGATTATTAAAATATTATTTTTTACTTAAAAAATAATCTTTCTGATTTATTAAACAATATTGGACAAATATATTTTTTGCTTAAAGTACGCCCAATGGCGTTGTTTAATTAATCTTTCTCACCACTTATTAAATAAACGACTGCTAAAACGTCCTGATAGCGTTTTTTAATTATTTATTTGAATTTTTGAATGTTAAATTTCAAAATAAACTAAGCAAGCCTGATAAACTTGCTTTTTTTATTGTCTTTTGGTATAATATTATTGTCCATTATCTGGTATTTATATTAGGTCTTTGGGCTATAATTCTAAATGTATTATAATATTGTTGAAAACAAAAAACACGCTCCGTTAAAGCGTGCTTAATGTGTTAGTTTAGGTTTTATTATCTTATTTTCTTAAACTTATTAGGATTTGGATTATTGTTAAGATTATTACTATTAGGTCTGATAATTCCATAGAATCACCCCCTTTTTTTAAAAGGTTATGGTGTATTCCTAAACTAACAATAATATTATAATATATTTTTTACACTTTGTCAATACTTTTTATATATAAATATATAAAAATGTGGTATAATTTAAGTGGCTATTACTTATTTACGACTGGTAGTAGCAAAATCTTTATTATTTCAGTTCCTTAATGGTTGAAATATAAAAAAGCCACCCTTTAGCAAGGTGGTTTTTATTTTGTCTAATAATCGCTTTTAATACGTTTTAAATATTCGATCTCTTCTTCTAATTTGCGTTGTTTACGTCTTGATAGATAACTATGTCTGATATCATCAATAAACGCCTTTGGATCTAATATACCATCCTTAATTAATTTGGCTAGCATTAATCTAAATCTTAGCATTAGCATTAATTCAAATACTTTTAATGGAATTGATAACGCTGTTATTAAATATCCAAGCACTGAGAACACTAAACCAAATAATGCGGTTGGGTCTTGTCAGTTGATGTGTTTTAATTTATCAAACAAACTCTTAATCTTGTCATTGAAATTAAAAAATGGTACAAACACAATAATCATAGCAAACGCAATTACAAATATTAAAATAGCAATTCATAACATCAAGCGTTTTTTATTAAAGAAAAATAATTCCTTAGTAATCGCTTTGAACTCTTGTTTTGTCATCTTTTATAATCTCCTGTTCTAATCGCTTAACTTTTTGTTCTTGCTCTTGTATTTTTAATTGCTTTTCTTGTTGTTCTAGTTTTTTTAATTCTTCATCCAATGTTTTGTAATTGTTAGAACTTATAACTGCTTTAATATTTTCAACACTAATATAACGATTACGTATTTGTTCAACTAGTATATCTAAGGCTTCGAACTCTTCTTTACTTAAATCCCCGTACTGGTGCATTTTAGCAACAGTTCGCCATAATGTTTTTAATGTTGGTAAAAGTGTTAACATTGAAATAATTAAATTAATAAATCCAATAGTCATAAAGAATGGAAAATAAAACTTTTGTTCTTTTACAAATAACACAAATGCGTTATCATTTGGTAGTCCAGCAATTAAATAAGTAGATAATAAAGCTAATCCTAAAAATAACCCTGAAATAATTGAACTTGTTAAATAAATCAGCTGTACTTGTTTTTTAGAAAACTTCCATTCTTTGTTTTTAAACTTATTTTTATTGTTCATCATTTAAAATACTCTCCTTATTTTGCTTGTATAAATCATTTTGAGCCTTTTTTATGCTTCTTTTTTGTTCGTTAATAGCTTTTCATTCACTTTTTAGTTTGTCTTGTTCTATGCGAGTTTTTAGATGTCAGTTTTTGCCTTCTGTTGTAAATCGTCAAAAGAACGGCGATATTGCGAAAAAATCAACGCAACCACAAATGATATAAGCAAATAAAACAACTAAAAATAATTGCCATTTTTTCGTACTTCCTAGTTTTGGAAATTTTGATATCATTCAGTCTCATAATGTGGCTTTGTCTGTATATTCGCTTTCTTTACCAAAAAATACAAACGATGTCTCACCAATTTTAATAAAAAACGAACAAATTAAAAAAATGGTTCATAATGTGGTTAGAATTCATAGTCATAAAATACGACGATTAATATATTCTTTCATTAAATTATTCTCCTACATAAATTGTTCTAACACTTCATTCATAATTTAATCCAACTTCAAAGTTTGTAGCAAATGTTTTATTTACTATATCTATTAATCACAAATCAACATTAACACCAGATTTTTCAGCATTAACACGAACGACTAAAAATTGAGGCTGATATTGATTTCACAACAGGATTTGTTGTTGTGTTTCTCCACTATCAGATAACATGTTATTACGGTCTGTGTTTCTATCAAAAGAAAACGTTAAACTATAATCTTTATCACTTCAATCAACACTACTGTTATTAAAATTTAATGATAGATACCATTTAAAAATTACTTCATGATTTGCCATATTTGGAAACGTACGTTTAATATCAGGTCATGGTATTGTTTTATTATCGCCATATTCTTCATCACGACCAGTTCATTTAAAATTCTCTGGTAATCGAACACGTCCAGCATAATATCATTGTGAATGAATTTTATAGTTGTTACTGCTTTTTTTGTTTTCTAAGTTTTTAATCTTAGATTCAATTGTTGTAATAGTTGAACTATTAGCATTATTTGCTGTTTGTACGCTATTAATTTTGTCGTTTAAACTTGAATAATTACTTTTAACTTTATTTTCTAAATTAGATAATGTACTTGATGCTGTTGATTGTTTATTTTCTAATTTAATATACTTTTGTTCTAAATTATTTTTAGTTTCTTTTAATGATGTTGCAATTGTAAAAGATTAGATGTATTTTAGCAATTGGATTATTCATTCTGGAATAATACCATCATTTCAAGTTATCAGTGTTTCAATCTAATCGTCTAGTTTACAGTGCCAAAAGGCAACTGTATCAGAACCTCTCTATTACCAGCAGATACGTCATTAATTAAATTATTTTATTTAAGTTATCCATAATTTTTCACGGAAACCTAACATTCCTATTTAGTGCAGTGTTTTTAAAACTTTCTAAATTAGATGTTAAAGCATCAACATTAGCACTATCACCTTTTTCACCCTTTGGACCAGTTTCACCTTTTGGACCAATTGGACCTTGTATTCCAGGTTCGCCTTTTTCACCTTTAAATACACCAGATGCTTTATCTTGCTCATAAGATGATAAAACCGCTTGTTTGGCTTCATTAACTTTATTTTCTAAGTTTTTAGTGAATGTATTTTCTTTAGTATCAATTGCTTTTTTAACTTCGTTTACTTTATTATCAACAGCATCTTGCACGCCTTCTTTAATTTTGTTTAAATCAATTTGTTGAATATTAATTAATTCTCATACATTTTCGTTAATTTGCTTACTTGGATCTACGATATTATGCGTACTTACACCATTGTTAACTAGTGCCTTATAAACTTTAATTGTGTTAGTTTTTTCATCTAAGTCATAAACTAACTCACCAGCTTTATAGTCATAAATAGCATCCCAGTACATTTTGGAATTAATTTTATTACAATATTCATACAATTCTTGGCGTGTAATTGCATTGTTATTATTTTGTCCATATCATGCTAAATTTTTAACGTCATGAATATCAACATAATTAGCAATAGCATTATCATAAACTTTATTGCTTTTATCTGTTGTTAAATCATTTGGTAGTGTACAATCATTTAAAATAAAGCCATTTAATGTTGCTAAATTACCAGATGCGTTAATGCTACTTACGTTATTAATATTGTGATGGTTTAAATCAAAATTATTATTAACAACAGGGTTATTTTTAGATATATAATGGTCTCTTAATTCTTCATGAACATTATTAATCGCATTAATTTTTAATTCATCATAATTAACATAAATACCATCATCGTTAAAGTTAGCAAACTTTGATTTTTTATATAATCCACAAACACGTAATTTATTAATGATATCTAATCGCTTTTTTTCAAAATCAGCATTTTCAGATTTAAAAGATAATGATAAGCCATCACCAATAGCAGATGTATAATCTTTATCGATAAAATGACCTTGTAATCATAAATAATTTGTTAATGTTGCAATTGCTTCTTTAAATTTAGTTTTTTGATAATCTGTTGTTTTTAAATCATCAAATCAACTATAAATAAAACCAGCACCACCATTTGATGCTACACAATCATCAATCATTGATTGAGCACGCAAAATAAACGTAGTTAACGCTTGTTTTTTACGTTCTAATTGTAATTGGTTATCTTCTAAATTTTCGTTTCGTACAATTAGTGGAAACAAACTTAAATATTCTTCTACACTAAGTAATAATCTATTATTCATTATTTAACTCCTTCAAAATATTTACGAGATGCATCAAATCAGTAAATACTTTCTTTTTGTAAGTGAATTAAGTTTTTACGATTAGCATGGTAAAATATGTTTCAAAATGTTGTACCAGTTGGTGCAAATTTCAGCATTGTAGCAATGCGTTTTGGAATATTACGTCATGTATAACGTGCTAGTGGATTACGTTTGGATGGTTTCATTAATTTAAGTGTTAAATAACCAATCTTACCAACGCCATCACTTGCGTATCATGTGCCATAATCTATCCAATGTTTATTAAAATCAATGTCAACATTATCAACATCTTTAAACTGATTTTCTTTACGCACTTTTTCAAAATTCTTTTTTTCAGTTTTAGATGCGTTATCTAATGTATCTGTTAAATGTTTGATAATGCTATCTTTTTGTTTTGTCGTGTATTTACTAGTTTGTTTAGCAATATCTTTAATCGCTTCATTAATAGCATCTTTTAGTTTTTTAGCTAAATCTTTGTTATCTTGAATAATTTTTAATTCTTTTGAGTTAAAAATCTTTTCTAAACTTGAATTATCAAAAAACTTTTTTAATATGTCATCTGGTTTTAAATTAAAGTCATGAAAAACAGTTTTACCTTTTTTCATAAAAGCACCAGTTTTATTTTTAAACATTTTAAAAAAATCAAATTTAAACATCTTTAACATACGTGAAAATAAGTTAATCAAAGTAGATATTAAACTCTTAATTTTAGATAACAACAAGCGACCAACAAAGCGTAGTATTAGAATTAGTATACCCATAGTATTAGTTAACAAAAAAAGGGCATTATTTGCCCTTTCTCCTTTCTTTGTAAAATTACTTGTAAGTACTTAAACCCCTTCAATTTGCTTTATTTACTTCAGTGATTTTAGAATCTTCACTGATTGGTGTTTTAAATTCAGCATATAGTGAGGGAAATTGTGAATAAAGATTTGAGCGTGCTTTATTGATTTCATCTTTTGTTGGTTTATCATTTAAAATCACAAAATTTAAATGTGCAAATTGTGGAAAGACCGCACTGTTCATACTTCATGAGTTATGAATTAACTGGTGTGATTTGCTTGTTGGACTTTGAAATGTTGTTAACATTAATTCTTGTTTTAAACAAATTGTAGTCTCAGGCATTGTAATAATACCGCTTAGTTTGCTAAACTTGTAATCACGAATTAAATTACTTGTGTATTTTTGATCTGTTGATTTTAAATAATCTTCGCCTAAATATGCAGATGAGTATATTGGCATTCCTAAATAATCACCCATATATTGTGAACTACTTCCTAAACCCTTAGTGCTATAGTCGCTGTATGCTTGACCATCACGAATTTTATAAGTTTTAGCAGTTAATACAACTAATGCTGCATCATCTGATAAAACGAATCGTAATTTATTTGGATCAGCTCCTAAATTAAATTTAGTACGACTTTTTGTTACTTGTTTTGCTTTACGATATATTTTTTCAACAATTTTTAATCGTTCATCATCTGTTAATGATTCAACACGATCAGGACTAAGTTCTGGAATAATAATATAATTACCAGTTGCTAAACAAGATTGATAAATCGCATCAATGTTAATTAAATTAATTTGATCTTGTTTAACGACTTCTAAATTTGCTAATGTCTTAGCTAAAAAATCGTTTCATTGAGCACCTGTTTTTTTATCAAATACACTAATACTTACTTCTTTTTGATAGTCATTACTTAAATGTAATGTTAATCTATCAATTGCTGGTACTTGCTTTTGTGTAAAGTCATCACGGTACTCTTCAACTTGTCCGATTACGCCAACTTCATAAATAGCAGTTCGCATATCATCCATAATTTTAGTTGGTGTCATTGTTGCACTTCCTAGAAATTCAGCGTTAAATTGATTTTCTAGCATATATTTTTCAAATCCACCTGCAACAACACGTTCATTATTAGCGTTAACAACATCTAAATATTGAGTGATTGTATATTTAGCATTTGGATCTGGTCTTTTTCAAGTGTTTTGGTTATCAGCCATTTTTTCAATTCTCCATTAGTTAAAAAATTTTAAAAAGTGGCTATTAGCATCTTCTACGACTGGTTTTTCTTGATGAATATTAACAAACTGTTGATTTTGTTCAAGCGTTGCGATTTTGTTTAATAATTCTTGTTCTCGTTGCTTAAACGTATTTTCTAAGTCTTTGATTTCTTTTAACTTAGCTTCAACTAATTTATTAGCATTATCTAATTGTTCATTTGTTTTTAGATAATTTTCAAATTGTTTTTGAATACGTTTGTCAATTTCATCATATAAAGCATTTTCTTTATTTTCTTCAGTTTCTTGTGGTTGAACTTGATTTTTTTCAAGTTCATCAACCATATTTACATACCTACTTTCTTAGATTGTTTTATTTTTTGTTTCACAATATACGCAACTTTTGAGATTATGAAATAATCAATTAGCATAGTTCCTACAAATCCTAAGCCAAACGCTACACCGATAATGGCTCGAGAGAATACATCAATACTGTTTGGTACTCCTCTTGCGAATGGTTTATCAACTAAACTAAAAGAGTAAAATTTCACAATTTTTTCTAATGAACTAGTTAATAACTCATATTTAGCAGTGTTTTCAGTATAAATTCACTTAACATTATTAAAACAAATTACAAAAAATGAAATTGCTAATATAGCAGTTAGAATTCATAATCACTTAATACGTTCGTTGTGAAATTTAATTATTTTGTTCATACTATATATTTTACTCCACTAATTCAATTGTTTTTAATTTAATGGTTTTTGATTTAATACAGTTATTTGTCTCTAATTCTTTAATAACATCGCTTAAATCAAATGGTGTTGTTTTAAATAAACGTCGTTCGTTATCTTCGCCTTTAAACTCATCAATTGGTTTATTATTTTTACTTATTTCTTTTTCATTACCCCTATCATCAATATAAATATTTGCCTTATTAGCATCGCTTAAATCAAATGGTTTTTCAATTTTATAAGTTGGTAAATAATTTGAAACTAAACGATAATTAACACGATTAGTTGGCTCGATACTATAAACAATATCTTTTGTTGTACTTTCATAATCTTTTAATTCAATTTTAAATGTGTTTTCATTAATTTTAGTTAAGATAATATATTTGTTTTCATTTGGTTTAACACTAACAGATATATTCTCTTTATTATCAACAACAGAATTAACACTAAATTCTTGATTAATAGCTACAAGATTAAATTCTAATTTATCATCAACTTCACCTAAATTAATATGTATTGAACTATTGTTTGGTTTTGAAATTAGTAAATCATAAACATCAGCTTTAATACTAAATGTACTAATTGTTGATTTTTTATTTGGTGTGTTTAAATCTACTGCATAATTACCACCATATTTTAATTTATTATCTTTTGTTAGTTCAAAACCAACAAAGTTATTAGCTGATTGAAACTATTATCAACGTTAAACTGATTTGTTCATCCTTTCTGTATCCAATTACTTTCAATCTCAAATGTTAGTTTTAAATTACGTTTTGTTTTATTAACTGAACCTAAATTTAACCATTCACGATAATTAAAATCTCATTTAAACACAAAACTATAAGCAATAACTTTATTAGCAACAATTACATCACTTAAATTACTTCCACCAAATAAATAATTTAGTTTATTAGTATATGAACCATAACCAAATAGATTTGCTAATTTAGAACTGTCTGGTTCATAAAAATAAAAGCGTGGTTTTCTATATTGCGTAGCACTTAATAATGAATTATCGCCCACTTTATATAATGTTTCATTAATTGTTTGTAAACTTGCATTAACTCCATCATCACTATTTGTTTTAGTATTTTTTAAAAAATCAGTTTCAACTGGTGGCTTTGGATAACTTAGTTTAGTTCCAACTGAATTAATCTCATCAAGCGTTGAAAAATTAATATAGTTGCTTCATTGACTCATATCATCAGTATATTTTCCATTGCTTTCAAAAATCGCTTCGTACACGTTTTTAAATTCGTCTTTTAATCGTGCATCTGGTGAATAAAAGCCACTAAAAGCTGTTAGTTTAATATCACACTTACCCAAGCCTTTAATATCAATCGTATCAATTACATAAGGATATTTAACACGATTAACATCTTCACGTTTACCAGACGCTATATTAATAACAGCTGGTTCATAACTAAGCGGTGTGTTTGGTGGTAATGCTTCACAGAAACTATCTCAATATAAAGGTTTTTTATCTTTTCTTATATACGTACCATCACGATATGTTGTTTGTCCAATTAGTTCAGTATCAAATACTTTTAGCTCATCATCTACAAATTTAGCAAATCTATCAGTTATATCAAAATGATAACAACAAGCAATGGCTGGTATGCCAAGTGGACTAAATGTTTTAGCTGGTTTGTTAGTGAAAATATCTAAATTAATTAAATCCGCTTTTAGTTTATTTGATACTTCTAATTCAAGCGGTAAACATGGTGCAATAAAATTAAAACCACGTGTATTTAATCAAATGTTTGACATGTTATAACCTGTTGCGTCTTCAACTTTGGTTCAACCAGGATATAAAATATCTACAATACCACGTCTAACTTTACCTAAACTAAGTGGTGTATTATCTATTAATTCACCAAGATTTTGACGGATTGTTTCCTTGTATGAATATGGTAAATACTTAATCAAATCATACATAAATGCATTGTGATTTAAAAACGTATGCAAATTAGTACCAGGTGGCATTGTAATGTTGTTTAATCCAATTTTAGTTGCTTCTTTTATTTGTTCAGCTTTGGTTTTAGCAAATCCCATATTAAAAGACCATAAACCTAAATTATTTAAATTAAATCCTTGTGAAACATTACGTCCAATACTTTCAAACACTGTACTCACAACTTCACCAGCAACTTGACCAGTTACAACAGCATCAGCTAACGGCTTTTTTTGTTCTGCATTTTCAGTTAATGATCCGCCCTTTTGTGCTCCTGTTCCACCTAATTTAAAGTTAAAAAAGCTCGCATGTATTTTAAAACCTTCATCTCATAATTTAAATGGATAACGATTGTCTTCATAACTTAGTGCGTTATTGCTTGCGTCTCATTTATGATTTAATAATAATCCACCATTTAATTTAATATTAGCGTTATGGTCAAATGTAAAATCTTTTTGTTTTTCATACTGGTCTTTATAAATCTTTTCAATTTCAAAAAACGCATTGTATAAATATACATAATTACTTGTTGGTGCTGATACATTAAAGAATTTAGATGCGACTGGTATTTTAGCTTTATATAGTAATAATCGCTTATTAGCACTTAACTCTCGTGTGTCATTATTATAAGTTCGTCCTGTTATTAACATACTATTTAATGCATATGGTCCATTAGTTAATAATTGAATACTTTTAACTCCTAACGGTTTTTCTTTACGTTCATCATATGTTAATTTACCATTTTGAATTACTGGTCATAAATAACCATCTCCAGGTTGACATGGTTGAATTGTTGCTAATATATCATTACCAGATGATGTTAGTAAATCGTTAACGTTTGTTAGTGTAATTTGTGCTCCTTCATAACGTTTTGATGTTGTTGAATAAAAAGGAATGACTTCATTAACCATTAATCATTGATTAGCTTGTGCGTGTTCATCTGGAATAATGAATAAATATTCTTTTAACATATCTGTAGTTCATTGTTTAAACTTTGCTTTATGACTAACTAAATTACCTTGTTCATTTTGGTATATTTCATCTTCATAAACACTTGGATATTTAACAATTAAAGTAACTGTTGTAATTGTTTTAATAACTGTATGTTCTTTATCATTAAAAACAATTGTAGATACATTATTTGCTTGGGAATAATTTTGTACTCATGCTTGAAAATAATAAGGTTTAAAGTTGTTTTTTAGCGTTGTATTACTTGGATCAATACGTGCGTAATCTTTACTAATGTAATTTAAACTACCTTTAAAGTTATACGTAAATGATTTATCTCAATACGCAATGATTGGCTCATTACTTGCTTTAATATTTCAAACAGTGCTAAAATCTTTGTCATTTACTAATAATGATTGTTCACCGGTGTTTGTAATTTTAATAAAACCTTTTAGTATTCTAAATGGACATAAATGAATTGGTTTTTGGTTTGGTTCAATTAAACTATCAATGCTGTCTTCAATATTTTTTTGACTATAACCTTCAAGTTCATCTAAATAAATATTACTCATTTTCAAACTCTCCATTTTCTAGTTTTTGTTTATGTTCTAATTCTTTTACTAGTTCATCTTGTTCGGTAGGTGTAAGTTCTGGATATAATTTTTGTACAGCTAAATTAAGTGGCATTAAATTAGCACCATATAAACTAATGACTTTTTCCATATTATCGTTGTAATCTTTAATTAAATTACTAATAATTTTAAATTCAACATATTGATCAAACTCTTGACGTATTAGTTTATTAACTTGTTTTTGTTCAATTGAGCTTAAACGCTTGTTACCATAACGTTTTGTTTTAAATCATGCAATCGCAATATTTTTAAAAAAATCTATTCAATCATTGGTGTATAGTTCTACTTTCATTTTTGCGTTTCAAAAGTTGCTTTATTTAAATTAGTTGCTGTTTCGCTATTTTGATAGCTTAAACTATCGCCCATATTTCATACATAACCACAACCTTTAAAGATTAAATTTAATTTTTCAGTAATGGCTTGTAAGTGAATACTTTCGTTAAAAGTAGATTGCATAATTTCAAATGCGTTTTTATCTGGTGTGTTTGTGTTTAATATCAGATCTGAACTCATGTACTGTATTTCATCAATTCATTGATTACGATAAGTTTGTGCGTCATTGTGATTAATAATTGATCCAACGATTCGTGTTTTGTTTAATTCACGTTCTTTTTTAATAAAGTTTAATAAAGCATTAGTATCATTGATTAAATCATTAACTGGATATCAATCTGGTAATTTCTCATTAATTTTATTTTCAAAGTCAATAAATGAACGATTTAAAAACTCTTTTGTGCACAATACGCCGTAGTTGTGTACGACGTTTTTGTTTTTAAAAATATCTTTAAAATACATTGGTGTAGAAACTTCAAATTCATTAAAAATAATTTTATATACACCAGTTGAGTTATAGATTAATCGTTTAATTTCGTATGGTGTGTGTAGTTCTTTTAAACTTAAATATTCACTATCTTTCATTAACGCAATTTTACGATAAATAGTACTGCTATACTCTTGTTGTTGATTAATCCTAATGGATTTGTTCATAGCGTTATTAATATCTGCATTAAAAATTAAAATATCATTGTTATATATTTCAAAACCAACATAGTTAATACCATATAGTGATTTAATACGACAATCACCTTTAATTAGTTTTAGTATTTCGTTGTTTTCATTAATATTATCAATAAATTCCATCCACTCCAAAGGTACATTATCTTTTGTTTTTAAGATAACATCATTAACAGTACTTTCTTGTGCAGTTCGTGCAATCATTTCTTTAATGTCAATGTTAATATCAAAATTAGATGGATTTCATTTACTTTTAATAATTGTATTAGTTTTAAGTGCCATATCTTATAGCTCCTTTTTCATTAATTGCAGGTGCTTGTGCATAAAAATATAAATGAAACGCACACATTAAATACTCCAACGCATTAATCGTATGCGTATGCTCATGACTACGCTCTTTTTTAGTATCTTCTTTAATGGTTTTTGGTAAAACAACGTAGCAATTTTTTAAGTCCTTATATAGTTTTGGACACGCATTTTTACTAAATCGCAATTTACCACTTGCAAGCATGTAATTAACTAAAACAACACGGTCTCAAATAGCGTGTTTAATAGCAGGTGCAAAAACAAGTTCAGAACCATACATATTATATTGAAACGCAATGCGTTTAAATGTAGCGTTAAATGTTTCATAAAAATAACGCAAATGAGCATTATCAATATAAATTACAATCTTAACGTTTACTTCTTGTTGTTTTTTAATAAACCAACGCACAACTAACTCAATTTGTTGCTCGCTTGTTAGTGGATTAACATTGTTGTTATAAACCATTTCATCTAAAATCTCAATTCCATTTTCTTTTGAAATTGAACCAAATAAGCACGCAGTATCTGATGATGAACTACTTGTTCCATCTCCCCAGTCCATACCACCAAAAAACTGCATTGCATAATTATCATATTCGCCATATGGTTCATGCATTACGCTTAAACTTCGTGCATAAATAGCACCTTCAACACTCCCACTTAATCCCAAACTAATTACACGATATTTAGAATAATCAATATCTTTTCATCATTCAAACTGCTCAATTTCTTGTTGTGATGTAAATTTATTCGCACGATATGTACTTCTTAAAAATAATTTTTTATCTACAACGCTATATTCATAATATGGCTTTTGTTGGATTGAGAATTCGTTTTCTGCTAAATGATTATTAAAATTTTCAATATATCAGTGTTCACTTGTTCAAGGATTAGATGCAAAAATAGATACGACCTGCGTATCTTTGGTACCCCTAAGTGATTGCTGTGAGTTTTTAACTAAATTAAAATCTAATTGTGACGCTTCTTCAAAAAACGCAAAAACATAACGTGCTTGCGTTCACAAACGAAATCCAACCTTAGCACCTTTGTTAATGTTATTACGTGTCTCATTTAATGCTTCAAACATTACAGTTCAACCATTAATAATAACAGTACGTTCTGAATGGTTAATCTTAGCAAATTTTAATAATTGCTTTTGGTCTAAAAAATTTAAATAGTCGCTAAAAATTTTTTTCGCATCAGATATATTCTGACGAAAAACATATATTGCGTTTTGCTGTTTAATCGCAACAAGTGATGACAAAAAATCTAATGTCGCAAAAGTCTTACCACTACTTCGTCCACCTAATAAATTAATTTCATTAAATGGTATTGGCAACTTTTTGGCTACAAACTCAACTAAATCACTATAGAAACCAAAACATTCTATAAAATCTAGTTTTATTCTCATAACGATATCAAAAAGCGTTAAACTAAAAACGATTAGTCTAACCTTTTTTTATTAAAATAAAGTTTTAACTATTACATTATTTTAAGTAGCTTTAATAATAACCACTTACCACGTAAAAACTTTTAAAAATATAATGTGGCCTACTTTTGGTTATGTCTCGTGCTTTTATCACCAAAGAGATAAGACTAATAAATAATTTTCACACACAGATACTTGACAAACGTGCTCTTAAAGCTAACTTTGTAGCTATTATCATACGACTTATTTTTGTAAGCGGCAATATTCAGGTGCTTTAAAAGGATATCTAACATATCTAAAAAACGTTGCTCTTTGTTCGTTTTTTCTAGTCATAAATATTATATCACATTTATAAGTTTTTTAATGGTTAATTTGTTATTATCATCAACTTTTTCAAACGTTATTGTTGTATCTTCAATATCTGTACTTTTTTGCTCATTTGCTAACTCAAAATCTAGACGTTTAAAATATTCTTGCGTTCTAAACTTACGCAATCCTTGGTTGTTTATTTCTTTATCATACGATGCGTTTCGTCCCAGCAACTCATTTAAAGCCATTCTAAGGCCGTAATTAGCCTTGGTAATGTATTCATACCTTTTTTTGTTAAACTCGTCTCTTTGCGTGCTTAAATTAACTCACACGCCCCAATTTATGCCTAAGAATTCAAGCAAAGCACTCAAATTACATAAATCTAGGTTTTTAGTAGTCATAACCAAAGTGTAAAAGTCATTGATTTTTAACTGCAGTTCTTGTAATTGTTTTAGTTCGTTTCTTTTCATCATACAAAAATTATAACCACATCGCACATAAAATTTAAATTAGATCATCGTTTAGTTTATCAATCAATAGTACAATCTAAAACGTTATCGTAAGGAAGTAGCGTAATATACGCATATTGAGGATTATCGCAAAAGCGATAATACGAAATAGAAGTATATGAAGATACTTTCTTATGATAACTAATATCATTTTTTTAAAAGACTTTTAAAGTATATATACGATATCGTAGTCTTTTAAAAAAATATAATAAATATAAATAAGATAAGAAATAATAAATATGATGTATAAGAATAAATATACGAATGATAATGAAGTATATTTATGAATTATATATTATATTTTTATTTTTTATTTTGTTTATATTTTAATTTATTACGCGTGAGAGAAATTGACAAAAATAAATTGACAACAATAAAAAAATGTTGTTATAATATTCTTGTCGTTTCTAAATTGCTAGAAACCAAACAACATAAGCCAAGGTTCAATAATCAATATTATTTTTCATTTTATCTTTTAAATCAATTTCTAAACCATTAGGATTTAATAATTGATTCTTTGTTAACGATTTATGGACAACTTGTGTTATTCCGTCTTTTTTATTAATTTCAATATCAAATGTTAATACAGGTTGATTATCTTGAAAAGAATTTAATATACTAAAATTTTGTAATTTAATGTTAATTTTTTTATTAGCTTTATCAATTATTGTATTTAATTCTGCTTGTTGAATATATTGGTTTAATAATTCTTGTTGGTCTTCTTGTTTAAAATCAAATTTAATTTCATTATTATTTAAAGTAAATTTTGATATTTTATAGTTAATATTAGATGCTAAGCCATTTAATTTACCTGTAAGTATTTTTTTATTTGCATCAAATACTAATTCAGTAGCACTAATAGCAGAAGCGTCATCATTAGCACTATCTTTAACTTCTAAAACAAAATCTTTTTTATTAGCATCTGCTAATTTAAATTTACTAAATGTAACTTCAATAGTAGCAGTGCCGTTTTGAATTTTATTAATTGTTATTTTATTTGCAATCACTTCATCCTTTTTAGTTGATTCTGGTGTTGATGGGTTTTCTGGTACTGGTGTAGAGTTTTGATTAGGAACTTGTTCAAAAGTTACTGTTTGATAATTTAAATTATAATTTATATTAACTTTAATTTATATATATATATATATAGAAGTAGTTAACTCCAAAAATGATAAATTTATATAATATTGTTTTGACAATAATTAAATAAAATTGTAAAATAAATTAGTAAATGTGCTAATGCATTTACAGTTTTTATGTGTCTTAAAATAAATTATATAAATAAAATTGTTTTATGTACAATATACAATGTTCTTTTTAAATTTTTACAATTAGAATAATTTAAATAACAAAAATTAATCTATTAGGAGATTATATATAAATGAAATTATTAAAAAACAAAAAATTTTGAGCAATGTCATTAAGTGCGATTGCAATTAGTACAAGTATAGTTGCGTTCGCAGCTTCTTGTTCAAATACAAATATTAAATCTAAGTTAAGCAAGCAATTAGCACAAACAAAAGATCAAAAAAACCATTTTGCTGTTTATGAAATTGAAAATTATACTAAACTAACAGATGAAGAAAAGAAGTCATTAGAAAATCTTGAGTTTGGTGTTTCTATTTTGGATAAAGATGGTAAAAATCCAATAAATATTAAAGTTAAAGGAGTTCAAAAAGAAGGTAAGATTTACGTTAAATTGCCACGCAAACCAGAAGTAAATGAAAAATTACTTGTTGTACCAACTAAAAATATTTTAAAAACAGAAGCATTAGTTTTAAATAATGATAATTTAAATTATCAAACAGTAACTTTTGAACAAGTTCCTGATCAAAAATTAGAACCAAAAGAAGAAAAACAAACTAAAGCTGTTGATTATGAAAAAGCAATTAAAGATGAAGATTATGTAAAACAAATTACATTATCTAATTTAGAAAAATATATTAGTAATGAGTATCAATATTTAAATCCAAATAATAAAACACTAGATTATAAAAATCATAGTTTTGTTCAAAAGTTTTTAGACGAAATTGATCTTTTAGCACAAAAATTAGAAAGCAACGACCTTAATTTAGAAGATAAACAAATTTATGAAAAAATTATTTTAGGATTACGTTGATTGCTAGCAGAAGATAATAATGAAACTAAAAATAAAGCTTTAGAATTATTTAAAGATTACTTTACAACAATCATGAAAGCAACAAAAGTAAATGATAAAGTTCATTATGATCCAAGTTTTATTAATGAATTTTATGGTTCAATAAGCAATTTGTTTGAAGAGCATGATTTAGAGCCATCAGTGGAAATTGAATCTAATAAAATCCGTATTGTTAAAGAAGATAAAGATGTAATAAAAGACAAAACAGTTACTGCAACATTTAATTTATACAATGGCTTTGACTGATCAACATTATTAAAAGATGTTGTATTTAAAGAATATAAGAAAGATAGTAATGGTAAACCAGTAATAGATGAAAAAACAAAAATGCCAATTGAAACTGATAAAATAGTTGAAGATCCTGTAGTAATGCGTTTAGAAATTGCTGAATATCCAAAAGATTGAAACACTAGCAAAGAAAATCCTAAAAACTTTTCATATATTAACTTTAAAAAATCAGAAGTTTTAAAATCACCAAAATTAGTGTTTACTAAGGTATTTAATTTAGATAAAGAACAAAAATATCATATTAACAATGTAATGTTCAGCCATAAATTAATTCCCAATTTATTGAAAGCATTGGATATAAAAAACAATACAACTAATGCCGAATTAATTAAAAAAATTAAAGAAAATATGACTGCTATTGATGAAAAAACACAAACAAATTTAAATACAAATTACATTCCTTTGTCACTTACAGAAACAAATAATAAAAAATAATTAATATAAAAAACTCTAGTTAGTATCTTTAAAATAACACTAGAGTTTTTTATATTAACTTAAAATTAAATTTTTCATTTTAGCTTCATCAATAAGTTTTTTAATAAAATATTCAACAGCAATCATAGCACGGACATCATTTTCGCAATAAATACGAGCGTCATGTACAAATTGTTCTCATTCTAGATCAGTAATTGTATTGAAAAAACGTTTTGTTGTTTTTGTTTGACAAACCAAACCATTACCCACACTTAGTTCTTTGTAGTTTAAACAATTAACATCGTGATAAATTTGTGGAAATTCTTTTTCAATAATTGGTAAGACTTTTTTAATTGAATAAAAACCTTTTAATTCAGGAATTACAATTAATTGCTTTCCCATTGAAACTATAAAAAAATCAGCTAAATCATACATGTTTTTAATTATTTCGCTAACCTTTAATTCATATATTTCTTCATTAATGAATTCAGCCATTTCTTTAAGACGCGAAGCTTCAAAAGATTTGTTGTATACAATATATGAATATTCTAATCCCTGGTGAATTTTATCAATAATTGCTTTAAACCATTCATTATTAATTTCACGTGGATCAATAATCATATTAGTGCATTTTAAATTTTTAATATCACAACTTCCATCATCAATAATAATAGAATTTTGTGTAACAGTTTGGGTAAAAGGAAACGAATTATCAACAGCGCGAATTGCTGAATTAATTGATTCAAAATCAAAATAAACCTTTTTTGATTTCAATTTAGCAAATAAATCAACAGCAGCTTGATAGATCTTTATTTGATTATTTTTATATAAATTAAAAAAGGTTTGATTAGCATATGTTTTTAAAATATCAATCCCTAATATATCTTTAGTAACATTATCTAAACCAAATTTTTTAATTGTTAAAATTTTTCCACTATATTTAATAACATCATATCCGCTATATGCGTATAAATTTCTTAATTCTAATCAAAAATCATTATCCTTATAGTCACCCTTATCATTATATGATGGTATAAAATAGTTAGGAATTCTTTGTTCGATAGGCAAAGCTAATAAATTTTGTTTATGAACTAATAATTCGCTGATTACTTCATCAAAATCATTGTGTAATTTTTCAATGCCTTTAATTAATTCATCCATGGTTTTATTAAAACGTACATCATCTTTTAAATCTTCTGTTTTGTAATCATAAATTTTTTTGGCTTCCTTATAATCATTTATGCTACTATAGGTTCCATCGATTAAATCATCTATTAAAATACCTTTACAATGATCATAAGCATCAATATCTTCATAATATTTTTTATTTTCGTCTTTTTTTAAATAAATTGCTTCTCCACGCTTACGTTTACGTTTTAATTCATTAATTTCAAATTTATTAAATTGATTTGCAGCACTTGGTGGTAAAGATAAAACTACGCTTTTTTGATAATTATAATAAGGAGAAATTGTAAAATTTACTTGATTTTTTTTTAATTTAACATAATCAACAATGCACATTTGATAACTTATACATTTATTTATAAAATAAGGGTGTTTTGAGATAACTTGTGCTTGGTAAAATACATCTAAAATATGGTGTTTTTTAATTGAACTTGTCCCTTTTGTTTCGATAACAATAAATTCATCGTTCTTTTTGATAAAACAATCGGGTTTAGTAATTAATTTATCATTAATAAAAACAGGTTGAAAAAAAATGATTTTATCATTTTTATCAATTAATTCTAAAGTTAAATTAGCAAGCTCTTCTAATGATTTGTTTTTAAATAAAGGATCAGTTTCGAAATTAATAATTTTCTTAATGTAATGATAAGTTTTTTTAATATCTTCTTGTGATGCTTTATCTAATAAAATACCTTCAGCAATTTTAGGATTGTCCACATCTATATTTTTATTAATTTCTAATTGTGAAAGAAAATCAGTTTGTTCCTTATAAATTTCATATGCATCAATTTCATAATCATGTTCTTCTTCATCATCTAAATCCTCAAAATCATAAGAACTAACATTTTTTAACAATAAGTCGATTTTAGCTTTAATCTCAGCATTAGTGAAAAACCACATTGCTTTTTGCTTTGTATAATAACCAATGTAATCATATTTAGTAATGTATTTCCCTGTGCGCATTGATAACCTCACTTTATAACATTAATTATATATAAAAAAATAATGTTCAATAAACGAACATTATTTAAAAAAACTTTATTTATTTTCATTTAAAACATATTCTAATTCTCAAACAACACCATTTTCTACGTTTGTGTGTTTAGTAATGTGACGTGCTAGTAATTTAGCGGGACGTGTACCATTTTTCATGGCAAAGCCATATCCAGCATGGTTTAGCATTGTCAAATCATTTTCACCATCGCCGAAAGCTATACAACGATCTAATGGAATTCCATAATATGAGGATAAATAGCGCATAGCCATTGCTTTATCAGCAAATGATGAATTAATTTCAATAACTTCAAATTGGTCATCAACCCTCGCTGATCAGCTACGAACCGCTAAAGTGGGAGCAATTTGTTTAATATGATAAACCATAGAATCAAATAAAGCTTTATCGTTGACATTTAATAAAAGGGCATTAGCATCTCTAATAAGGTTGTTAATATCATTTTCCTTAGTCAATTTATTTTTTTCATTCATATCACGAATGGGCACGTGAAATATATCAATAAATGATTTTCAAATATTTAAATCCATATTACTGTTTAAAATGGTAGCAACATTATCAGCTTCAACAACAGCATTAACAATATTTTTAGCAATAAATCGATTTTTTAAAATTGCGCTTACAATATCTTTACTAAATGTCATATTAATTGGCGCGAATTTTTTATCGGAAGGGTTTGAAATATAAGAGCCATTATAATTAGCCATTAAAGTTGTTAATCCTAATTTTTCATAAATATGGATTGATCCACGTAAGGGACGACCTGTTACAATACAGAAAATATGGCCTTGATTGATGATTTTTTTAATTCCCTCAATTGTTTTTTCTGATATATCACTATCATTATTTAAAAGTGTTCCATCTAGATCACAAGCAATTAAATATTTGTTGTTTTTTCCCATTAATTTTTTACCTCATTATATACTATTATTTTAGTTGCATATGTTTTAAAACTAGTTGTACATTTTGATAAGCAATATCGTTAGCACGCTCGCAACTATTGTCTATCATTATATCTAATTCTGGACTATTTAATCAATAAGAAATTTTTTGTTGCAGCTTTTCTAAAAAATCAGCAACGATATTGGCTAAATCATTTTTAAAAACACCATAGTTTTGTTGTTGGTATTTTAGTTCGATTTCTTTAAAGCTTAGATTTGTTAAACAAGCATAAATTGTCATTAAATTACTTACTCCTGGTTGTAATGTTAAATCATATTTAACTTTGTTTAAATTATCTGTTAAAGCTCCTTTAATTTTTTTAAAAATTATATCCTTTGAATCATTTAAAAAAATCACACCCTTAAGGTTTTTAGAAGATTTTGACATTTTAATATTAGGATCTAATAAATCCATAATTTTTGCCCCTATTTTAGGAATATAAACTTGAGGCAATTTAAAAGTATTACCATATCTTTTATTAAAACGGTCAGCAAGCGTTCTTGTTAATTCCAAATGTTGTTTTTGATCTTGGCCAACAGGAACAACATCTGCATTAAAAGTTAAAATATCTGCTGCCATTAATGTGGGATATGTTAATAAACCAGAAGGAATCATTTCAGTGTTATTAGCCATCTTTGTTGCTTTAATACTTTTATCTTTAAATTGTGTCATACGCGTTAGTTCACCAATACTTGTTGAACATAATAATATGTGAGCTAATAATGGAATCGATAAGATATCTGATTGGTAAAATAGACATGTTTTTTTAGGATCAATACCAGCAGCTAAATATGTCGCGATAATCTGACGTTTAGTAGTAGAAAAATCAGTGTTATCAAAAATATTTGGTGTTAAGGAATGTAAATCCGCAACAAATAAAAACACTTCATAATCATCTTGTAAATCAACAAAGTTTTTAATTGCACCTAAATAATTTCCTAATGTTAAATTATTAGTTGGTTGAATTCCTGAAATTAGTCTTTTCATAATGTTTTATTTTACCTTACTTAAACGTTAATACAATTAAACATTTAAAATTAATGTTTTTTTTGTATACTTAATTATTATATAAGTAAAATTGTAATTATAAGAAGATAAAAATAAAAGGAATATGATTTACGTTTTATACAGTCCTAATTGCGCAGTGTGTAAAAAAGTTGTGCGATTTTTCCGAAATAATCAAATTGAAATTACCAAAATTATTATTGGTGAAGATAAAATAGAACGTTCTATGTTGTTAGATATTTTATCTCTTTGTGAAGATGGCTTTGGAACAATTATATCTTTTAAGACAGAATCTTCAAAACGCTTGAATATTACTTCAAAAACTTTTTTAGATTTATCTACAAAAGAATTATTAAATTTAATTCAAAATGATTTAAATTTAATTCGTCGCCCTTTAATCTATCAAACAAAAAACAATCGACCATATCGTTTACAAATTGGTTATGATTCAGAAGAAATTGAAATTTTTAAACGAGCAGTGCATGAAAAATAATAAGCCAATTTGTTTTTATGATATCTATTGTTTTAAACCACAAGAATGTTTTAAAAGAAATGATATTAATTTGTTAGAAGATAAACTAAAAAGATATTCAAAAATAACATTTTTACGAAATAAAGAAAATCCTGAAATTATTTTTTTATTAGGTGGAGATGGATCGTTTATCAATTTTATTAACCAACAATGAAAAAAAAATGTAAAAATTGTTGGCATTAATTATGGACAATTAGGTTTTTATAGTTCATATGATAGTATTAAAACTATAAATTTAGACGAAATTATTGATGAAAATATGTATTATAATCCTTTATTACTTAAAGTAAGTATTAATAACCAAAATTTTTTTTATTGTTTGAATGAGTTAAGTTTATTTTCTAATGAGTTAGTGTCTTTTGATATTAGCATAAATGATTATCCATATGAAAAATTTCGTGGCTCTGGTTTGTTATTTGTAACACCAAGCGGATCAACTGGCAAAAACAAAACAGCTTTTGGTCCCATTATTTTTAATAATCATGAGAATTTTATTATGACAGAGATTTTTCCTGTTAATCATTTAAAATATTCAAGTTTAAATGCCCCTGTGGTTTTTAGAAAAGATTATAAAATCAGTTTAACAAATATTAAATTTAAAAAATCTTTTAGTGTTGCTATTGATGGGAATATCATTAATTTTAGCGATAAAATTAATGATATTAAAGTAGAAACAATCCAAGCTTCATCAAAAATTCATGGTTTAAATAATTTTAAGAAATACATTGATAAATTAAACAAATCTTTTATTAAAGGAGAATAAAAATGCAAAATTTAAATAATAAGCAAAAAACATTAATTATTTTTTTAGGTATTATTACCTTTGGAATTTTTATTATTTACTTTTTTACAAAAGCTAAAAAAGTATCTCAAATTAGAAACACTCATTTAATAGTATCTTCAAATATTCCTTTTAGTTTAAATGATTTTTATAATTGTATTGGTTCAAAAAATAATTTAGTTAACGTTGATGCGACAATTAATACATTAAAAATTGAATTAAAAGAAATTAACGCATTGAATAATGAAAAATTAAAGGTTTTAGGTGCAAAAGGGATTATGTGCAATCAAACAAAAATTTCTATTATTTTTGGTGATTTTTGTTTGGAACTTAAAGAATTAATTAAAAAAGATTTATTTTCATAGTATACTATATACTTAATTTGTTAATGTACATATACAAATATTATATTCATAAATTAGTTATTAAAGGACATTAAAAAAATATGTTAATTAAACCCGCGAAACGCAGTGAAAAAATTTATACAATTATTGCTATAAAAGATAGTGTTGTAACTTTAAAAAATGATTATCAAGACGAAAAAAATATTCAAATTTACGAGTTTGATTTGCAGAATATTATTCCAGAAGTTGGTAAATTTATTAATCTAATTAAATACGATCAACAAGGTTGTTCAGTATTTGAAGAATATGATAAAAACATTTAATTTATTTATGATATTTTCCGTTATTTAAAATACTAAGTGCCCTATATATTTGTTCACATAACATGACTTTTGCTAAGCCATGAGCAAAAGTCATTTTTGACATAGATAAATGATAATTAGCACGCTCATAGATGCTTTCATGTAAACCATTAGATCCACCAATAATGAAACAAATATCATGAGAAAATGTGTTTAGTCATTGTTGTATTTTTTTAGCTAATGTTTCGCTACTTATAATGTTGCTTTCAATAATTAAAGCAATTACAAAATCATTTTGATTAATATTTTTAAAAATTTTAGAGGCCTCTTTACTTTTAATTTGTTCTAAGGATTTATTTGAAATTTGAACTGGTTCAGGTTCATCGCTAACAACAATTTCTTGGCATTTTAAATAATGGTTAATTCTTTTTAAATAATCATTAATTAGATCAACAAAAGCTTTTTGTTTTAATTTCCCTACACTTATAATTTTTATCATCATCTTAGTTAAATAACCTTTTTTTTGATTATAATCTAACTAAGATTATAAAGGATAATGATTCAATGAAATATATTATTGCTAATTTTAAAATGAATGCAACACAAGAATTAATTAATCATTTTTTAAATAATTTAACGTTATTTGATGAGCAAAAAATTATTATTGGTTTAGCACCAGGGGATTTATATTTAAAAACATTTGTTAATTTAGCTGAAATAAAAAAAGTCAATTTATATGCTCAAAACCCATCATTACATAATAAAGGACCTTATACAGGTCAAATAAGTTGTTTACAATTATTAGATATTAATATTAAAAATGCTTTAGTTGGGCATAGTGAAATACGTATTGATTTTTCACAATCAATAATTGATCAAAAAATAAAAATATCTATGGATTTATTAGAACAAGTCATCATTTGTGTTGGCGAAACTTTTGATGCTTATAAACAAAATAAATCACTTAATTTTGTTTTAAATCAATTAGCCAACATCATTAATTACAAAGGATTAAAAAAAATTATTATCGCTTATGAACCAATTTGAGCAATTGGCACTGATTTAGAATTAGATTTTAAACATATTAATTACATGATCGAAGGAATTAAAACATATTTATATAATTGTACTGGAATAAATATTCCTATTTTATATGGCGGTAGTGTTAATGACAATAATATTAATGAATTGTGCAATCAAAAATTAATCGATGGTTTTTTAATTGGCAATGCTTCATTAGATGTTAATGTTTTTAATAAAATTATCGATAAATGTAAATAATAAAATGGATTAGAATATGAAATTAAATAAAAAAATAGGTTTGATAATCATTGATGGATTAGGAATTGGAAAAGAAGATAATACTAATGCTGTTCATTTAGCAAATCCACCAACATTAAATTATTTGATTAATAATTTTCCCACATTACAAATTAGTGCGGCTCAACAAAATATTGGTTTACTTGAAAATCAAGCAGGTAACAGCGAAATTGGTCATTTAACAATTGGTGCGGGCCGTATCATTTTAAATGATAATGCAAATATTAATAATTATATGAAATCCGTTGATTATGAAAGTGCAGTTTTAAGCAATATTAAAAATGAAATTGTTCATATTGTTGGCATGTATTCGAATGGTTTAGTACATAGTAATTATGAACATATTCATTGAATTATTAAACAAATAATTAAAAACAATAATCAGGTTGTTTTACATTTAATTTCTGATGGACGAGATGACTATCCTTATGGTTTTGCTCAATTCATTGAACAAATTAATGCCTTAAAAAAACAATATAATATTATTGTAAAGAGTCTAAGTGGGCGTTATTTTGCAATGGATCGTGATCAGCGATGAGAACGTACCCAAAAAGCATTTAATGCAATGTTTGTTAAGCAAGATAAAATATGTGAAAAAACATTTTTAGAGGTTGCACAATCAATCGCAAATTCTTATAAAAGTGATGAATTTGTTCAACCTGTAGTTTTTAATGATGATGAAAAATATAATTTAAAACCACATCAAAAAGTGATTTTAGCAAACTATCGTTCAGATCGTATGCGCCAGTTAGCACACTTACTTAAACCTAACAAAAAATTTCATTACAATAATCCGTTTTTAGTTCAAGATGTTAATTTAATTACTTTAGTACCATTCCCTGATGTTGATGCAATAGCTTTATTTGAGAAGCAAAATTTAAATAACACTTTAGGTGATGTTTTTAATAAACACCAAATAAAAGAAGCTCGAATTGCAGAAACAGAAAAATATGCCCATATATCTTTTTTCTTTGATGGCGGTGTTAATAAATATTATTCGACAAAAACACAATATTTGATTCCTTCGCAAAAAGTTGCTACTTATGATTTATGTCCCCAAATGTCAGCACATTTAATTACGAAAACTATTATCGATCATTATTTCGATCATGATGTTTTTATTGTTAATTATGCAAATCCTGATATGGTTGGTCATAGCGGCAATATGCAGCAAACAATTGAAGCAATTCTAAGTGTTGACCATGAAATTCGAAAATTATATGATTTTTTTGAAAAGAATAATGGTGTGTTGATGATTACAGGTGATCATGGAAATGCTGAAATGATGCTTGATGATCAAAAACAAGTAATTACTTCTCACAGTGTTAATGATGTTTGGTTTATTATCACAGATAATAAAGTTATTTTAAATTCGACACAAAAATTTAGTTTAGCAAATATTGCTCCAACTATTTTGGAATATTTAAATATCGCTCAACCAATTCAAATGACAGCAACGAGTATGATAAAAAAGATTTGTGAATAGTTTTATTATGCTATTATAATAATTTGATATACGAAGGAGTTTTATAATGGATAAAAAAGCTAAAATTTATACTTGATCACACATTATTTTAATTGTTTTTAGCTTAGTTTGTTTGCATTGGTTTTTCATTTTATCAATCACAATTCCTAACCAAACTGGTTTTTTAATTAATCGAGTTGGTCAATGAATAAATTCATCTAGTACATATGCTTATAAAATTGATCCTCGATCATTATTTTATTTAAATTATACTTTAAACTTTAATATTGCTTTAAATGCAAGTGGTATAGCTAGTATTGTTTTATTTATTTGTTATATTTTAACATTTATTTTTAATCTAATAATGATTGGAATTATTAGATCTTGAAGGCCAAGTTTATTACATTTAATAATTGCTATATTAATTTGACTTGCTATTTTATATTTATTTATCATTATTATGATCAAACCAAACTTTAATCAGATTATTAATAATAGTTATTATACATGATTAAAAAATGTTATTTTTAATGATCAAACTTTAACACTTAATAAAAAAAATGTGATTTTAAATACTTATATTAATCACTATCATTTACCAATAATTAATGATCCACAAGTTGCTTTATTAGATATTAGCAAACACCAAATTAATAATGAAAATTTAACATTTAATCCTTCATACAACTATAATGCCAATCTTTATTTTACAAAAGCAAAATTAATTTATTGTACTTATACAATTATTGGAATAATTTTTATCATTAGTTTTATTTATTATCTAAGTGAAATTATTAAACGATGTTTATTAGTTAATGACAATTGAAAAATTAAACAACACGAACGAAAAGATGGTTTAAATATAAAAAAAAGAAAAAATAAACAAGAAATTGTTGCTCCTGACCCTATTTTAGAAGAAATTTTTAAAGAATTAGATTTATAAAAAATATTGAAGACACTTGTTTAATTATTTGTCTTAATATTTTTTTAATTTTTAACATAAAATTAATGTATAAGTCTTTTAAAAAAGAGGAATTAACAACATATGAAAATAATTAATTTATTAGCTTATCAAATTCTTGATTCGCGCGGCCAACCAACAGTAGCTGTTAAATTATTTTTAGAGAACGATCAAAGTGTAATTGCAATGGTTCCATCAGGCGCTTCCACTGGAGCAAAGGAAGCATTAGAATTAAGAGATGGTGATGTAAATTATTTTTTTAATAAGTCTGTTAAATTAGCAATTCAAAATATTAATAATATTATTCGCCCTCATCTAATTAATAAAAATGTTTTAAATTTTTTTGAATTAGATAATTTATTAATTAATCTAGATGGTACTGAAAATAAAAGTAAATTAGGTGCTAATGCTTTATTAGGTGTTTCTATAGCGATTGTTAAAGCGGGAGCAATAGCCGCTTCTAAACCTTTATATCAATATATTAAAGAAGACTTAATGCATAATTATGATGTAAACTATTATGCTCCAATTCCGTTAATGAATTTTATTAATGGTGGGGCTCACGCAGATAATGATTTAGATATTCAAGAATTTATGATTGTTCCATTAAATGCAATTTCATTTTCTCAAGCGATTCAAATTGGATCAGAAATTTTTCATCAATTAGATAAACTTTTAAAAAGCAATCATTTAAGTACAACAAAGGGTGATGAAGGCGGTTTTGCTCCAATGTTAAAAAATAATTATGTGACTTTAGAACTATTAGTTCATGCTATTAAAAAAGCACATTACCTTCCTTCAAAAACACAAGGAGTTTGTCTTGCCTTAGATGTCGCAGCTTCTGAATTGTATGAAAACGGAAAATATTTTTTTAAAAAGTCTTCATCTCATAACATTACTCTTGAACAAACATCATTCAGTAGTGATGAATGAATAAAGTATTGAAGTAAATTAGTATCTATGTTTCCTATCATTTCGATCGAGGATTGTTTTGAGGAAAACGATTGAAATAGTTTTGCGTTATTTTTAAAAAATAACCCACACATTCAAGTGGTTGGTGATGATTTATATTGCACAAACTTAAAGTATCTACAAAAAGGAATTGATTTTAAAGCAACAAATGCTATTTTAATTAAACCTAATCAAATTGGTACAATTAGTGAAACGTTAGATGTTATTAAATTTGCACAAAAAAACAATATTAATACTATCATTTCGCATCGCTCTGGCGAAACTGAAGACACTTTTATTGCAGATTTAGCGATTGGTGTAGGTGCTGGGCAAATTAAAACAGGTTCATTATCACGTTCTGAACGGATAGCGAAGTATAATCGTATTTTAGAAATCGAACAAGAATTAAAGGATAAATTAATTTATGAACCAAGTAAATTTTTTAAATTTAGATAAAAATATTTTAATTATTACTTCAGGTGGTGATGCACCGGGTATGAATGCTTCATTAATTTCATTAATCCATAGATTAATGAATAATAATTTTAATGTTTTTATAGGAATTGAAGGATTATTAGGACTGTACAATAATTTAATTGAACCAATTAAAGATAAACGTGTTTTTGATGTTTATTTTAACGAGCAAGGAACAGTCATTAAGACAAGTAGATTTATTAAATTAGATATTAATGATAAAAAAACACAAATTATTAAAGATAATTTATTAAGTCATAATATTCAAAAAATTATTATCTTAGGCGGCCAAGGTAGTATGCAAGCTGGTTTAGTGTTAACAAAAATGGGCTTTGAAGTCTTTGGAATATTACACACTATTGATAATGATTTTAGTGAAACACAAATGTGTATAGGGGCCTTAAGTGCTGCTTCTTTTAATCAAAAATTATTAAAGTGCCTAAATTACACAGCAAAAGCTCATTGTGCTTTTAATTTAGTTGAGCTTATGGGTCGTGAATGTTCATGATTAGTCAATAATAGTGTTGGTAAATTAAAACCTATTTTAATGTTAACTAATCAAGATAATAAATATACTGTTGATGAAGTAATAGATTTAATTAAAGATAAAATAAACTCAATAAAAGAATACGATCCTTTAATCATTGTTCAAGAATTAATCTATGATCAAAAATGATACGAATTATTAGTAAAAACTTTTGAGCAAAAATTACATAAAAGTTTACGAATTACAATTTTAAATTATTTACAACGCGGAGCTCCAGTAAGTGATTTTGATTTACAATTAGCAAAAGATAGTGCGAATGTATTGGTTGATTTTATTGTTAATCAAAATGATATAAAAAACACAAATAATATGTATGTAGTTATTAATAAGTTTGATAATAAGTTAGAAGTTATTAAATTCAATAAATAGAGGGAAAATTAATGTTTTCAAAAACACGCATTATTGCTACAGTAGGTCCAGCAACAACAAAATTGTTTTCTAATTGGTTAGAATTTAACGATTTAAATAATATAAATGATGCTCAACAAGTATGCATAAACATTAAAGATTTAATTTTAAGTGGTGTTAATATTTTTCGTATGAATTTAAGTCATGGCGACCAAAAAATACACCTTTTTCGTACACAATTAATTAAAAAAATTGCGGATGAATTAAAGATCAAAGTTGAAATATTATTTGATACAAAAGGACCAGAAATTCGTGTTTGTGAAATGAGTGATAATAATTTTATTATTAAAAATAGTGAAGTTATAATACATTGTAAAGAAAAAGTTTTAGGTAGTTTTAATGAGTTTAGTGTTACTGATGCTACTGGACAATACAATATGATTAGTGATGTTAAAATTAATCACCGAATTTTAATCGACGATGGAAAATTAATTTTAATTGTTAAAAAAATTGATTTTCTAAAAAACATCATTTATACAACCGCTAAAAATAGTTATTCATTAAAAACAAATAAACGCTTAAATTTACCAGATGCCAATTATAGTTTGCCGTTTTTGTCAAAGAAAGATATTGATGATATAAATTTAGCAGTTAAATTAAAAATTCCTTATTTGGCTTTATCGTTTATTTCTAATATTAAACAAATTAATGAGGTTAAACAACTTTTAAAAAAGTCCTCATTTATACCAAAATTAATTGCAAAAATTGAAACTCAAGAAGCAATTGATAATCTAGAAGAAATTATTAAAAATACTGATGGTATCATGGTTGCGCGTGGGGATTTAGGGCTAGAAGTACCTTTTTATAAAATTCCTATTTATCAAAATAAAATTGTTGAATTATGCCATAAATACAATAAATATTGTATTATAGCGACACAAATGTTAGATTCATTAGAACGTAATATTATCCCCACACGAGCTGAAGTCACAGATGTTTATTATGCCGTTAAACAAAAGGTTAACGCAACAATGTTATCAGGCGAAACGGCCGCAGGAATTGATCCAATTAATGCTGTACAAGTTATGAAATCTATTATTTTAGAGACAGAAAATAATAATATAGAAAACGAAATAACATTTAAAAAAAATGAATTATTAATTCCTAAGGAAGTTAAATTAAAAATTTTATCATTTCATAAATTAAAAATAAATAAACATAAATTATTAGTTGTTAATTTAATTAGTAAAAATTCTAAACATTTAGTTTATGATATATTAAAATATGATTTTAATTTTAATATTTTATTTGTTATAGATAAAAATCAAGAACATGATTTATTATTAAAAAATGCTAATTTTATTATTAGTGAAAATAAAATGTTAAAACAAGAAGAATTATTAAAATCATTTAATATTGATTTTAATAATCATGAATTTTTATTTATTTCAATTTAAAATAAAAATAATTTTATTATTAATATCTTTAATATAGTATAATTATAAAATATTCTGTCTATTTAAGCACAAATGTGTAATAAATAGGCTTTTTTCTATTTTTGTGAGGTACTAGTTAGAATATGCAAAATAATAAAAATTATACTGAGAAATTTATTACTGATAAAGTAATGCGAAGAGATTATTCAAAAATTAAATCTAATTTTGAAGGACCAAATCTACTTGAAATTCAAGTTGAATCTTTTAAACGCTTTATGGAGAAAGATTTAAAAGAAGTTATTTCAAGTATTTTTCCATTAAAATCTCCTCAGGGTAAGTATACGTTAGCATTTAAAGGTTTAAAAATTAAGCAGCCAACAAAAGATGAAAGTGCTTGTCGTGACGAAGGTAAAACATTTGAAACACCAATTTATATTGATTTGGAATTAACAGATAACTACACAGGTGAAGTTAAACGTGCACAACGAAATACTAAAACAGGAGAAGATGGTATTTATTTAGGAGCAATTCCCAAAATGACAGAAAAAGGAACTTTTGTTATTAATGGAATTGAAAAATTTGTTATCTCACAAATTGTTAGATCACCAGGAATTTATGTACTTGGTAAATCAAGCATCAAATTGAACGGTTCACGTAAGCGTTTATTTGAAGGCAAAATTTGTGAAATTTATCCTTCTAAGGGTACTTTGATGCTTGGTTATATTCCTAAGGATCGTCATAATATTCAAATTGTTGCACGTGATTCATCTGGTGATAATGCGCAAACATTTTCAGTAACTACTTTACTAAAAGCTTTTGGTTTAACAAGTGCTGAAATCTTAAAAATTTTTAATAATGAAAAAGAGATTCGTGAATCATTAGAAATTGAAAAATATGCGCCAGAATATATTTTTGAAAATTCACAAGAAAATGAAATTATTTTTAAAATTTATAGTGATGCTCATGATATTCATGAGAAAGCCGATCGTCGAGAATCAAATAATAAGTTAAAAGAAGAATATATAGAACAAGGTTCACCACTTTTATCAAAGTTAAAACAACTAATTTTTAATTATGTTGAAAAAAACGATGAAATTGATGCATTATTACATGAAAATAAAGATGTTGAAGATGCTAGTTTCATTAAGAATAATAAAAAATTATATGATGAAAGAGAAGAAATCATTAATTGCATCATTAGTGAAAAAGCAGCTAAGGATATCGTTGAATTATTAGGTATTAATATTAAAAATATAGAAACACTAAGACATTTAGGCAAAGCTTCTTATCAAATAGCTTTACAACAACATTTTTTTAATAAGCGTTTATATGATATTAGTAGTGCTGGACGCTATAAATTTGAAAAAAAATTATTATTAAGTGAACGTTTATATCAAAAAGTAATTGCGAATGATATTATTGATAAAAAAAATAATATTCTAATTCCTAAAGATACTTTAATTACTAAAGAACATATCGAACTAATAAAAAAAGAATCACGTGATAAAAACATTAAATGAACAAAAAAAATTAATTTATTACCAACAGCATTAGAATCAGAAATTGAACAATTTTTAGAATATGAAAGTATTGCAGTTTATAAAGATAATGATTTAAGAGATGAAACAACAGAGATTGTCGGTCTAGCATCAGGTTGCAAACTTCAAACTTTAACCGTGGCTGACTTAGTTGCTACAACAAGTTATATTTATAACTTGAATTATGAAATTGGTGAATTTGATGATATAGATCATTTAGGTAATAAAAGACTTAAATTGATTCATGAATTATTACGAGCAAGAATTGCTACAAGTATGGCTCGTATTGAGAAGTTTATTAATGAAAAGTTGGCTATTTCTGATGGTTCAAGCAATAACATTACAAATGTTAATGATAAAGGTATTGATACTGAACTTGATCGTGAAATTGAAGAAAGCGATATGAGTGATGAGGAAAAGAAAAAAGCAATTAGTGTTAAATCAATTATTAATACAAAACAATTCCAAAGTTTAGTTAAAGATTTTTTTAATTCACATCAATTAATTCAATTTATTGACCAACAAAATCCATTAGCAGAATTAACTAATAAACGTCGTATTTCAGCGATGGGTCCAGGAGGAATTAGTCGTGAAGACCCTAATTTAGATATTCGTGATGTTCATCACTCTCACTCTTCGCGTATTTGCCCGATTGAAACACCAGAAGGAATGAATATTGGGTTGATTATGTCTTTAGCATCACTTGCAAAAGTAGATGAAAATGGTTTTATTGTAGCACCTTATTATGTTGTTGAGGATGGGGTTGTAAAAGAAGATTATAAATATTTAACAGCCCATGAAGATGATAACTATATTATTGCTGAATCATCAGTCCAATTAGATGAAAATAAACGAATTTTAGATGAACAAGTTGTTGCTCGATATCGTGGCTCAACAGGTTTATTTAGTCCTAATGAAGTTGATTTTATTGATATTGTTCCAAAACAAGTAGTATCAATTGCTGCATCAGCAATTCCATTTATTGAAAATGACGATGGGGCACGTGCTTTGATGGGGTCAAACATGCAACGTCAAGCAACACCTTTAATTAAACCATATGCCCCTATTGTAGGGACAGGTACAGAATTTAAAATTGCACACGATTCAGGAATGGCTGTTGTTGCTAAAAATGACGGAGTGGTTGAATTTGTTGATAGTCAAAAAATTATCATTAGAAATGATAATGATAAATTAGATGATTATAAATTAATTAAATATCGTAAATCAAATCAAGACACATGTAATAACCAAATTCCAATTGTAAAAGTAGGTCAAAGAGTTCACAAATCTGAAACAATTGGTGATGGACCTGCAATGCAAAATGGTGAATTAGCTTTAGGTCGTAATATTCTAGTTGGTTATACAACTTGACGAGGTTATAATTTTGAAGATGCTATTATTATTTCTGAACGTTTAGTTGATCAAGATGTATTTACATCAATTCATATTGATGAGCATACAATTCAATGTATGAAAACAAAAAACGGTGATGAAGAAATCACTCGTGACATGCCTAATGTCTCTGATACTGCAAAACGTTTTTTAGATAATCAAGGTATCGTTTTAGTTGGCGCTGAAGTTCATGAAGGAGATGTTTTAGTTGGAAAAACAACTCCACGAGGTAATGTTGAAACTGCTCCAGAAGATCGTTTATTGCAAACAATTTTTGGCGATAAATCAAAAACAGTTAAAGATTCATCACTAAAAGTAAAACACGGTCAAGAAGGAATTGTTGCTGCTGTTAAGCGAATTAAATCAAGTGATGAAAATGGTAGTGAATTACCAGATGACGTAATTGAAATTATCAAAGTTTATATTGTTCAAAAACGTAAAATTCAAGTTGGTGACAAAATGGCAGGGCGTCATGGAAATAAAGGAATTGTATCAAAAGTTGTTCCTATTCAAGATATGCCTTTTTTAAAAGACGGGACTCCATTAGATATTATGTTAAATCCATTAGGTGTACCTAGTCGTATGAATATTGGTCAAATTTTAGAATTGCACTTAGGTTATGCTGCGGCTGAAATAGGTAAAAAACAATTAATTCAAATTGCAATTGATCAATTAGGTTATGAAAAATATATTTCATTGTTTGGAATCAATGAAATTATTGCTAAAAAACTTTATGAAAATATTAGTAATTTAATTAAACACAAACAAGCAAAACAGGCAAAAGATATAGATTTAATTGATGTGACAATTATTCTAAAAGAATTGGGATTATCATATGATGATATTGGAATTAAAATTTCAACTCCTGTATTTGATGGTGCAAATCATGATGATATTGTTTCTATTATGAATGAAGCAAACATTGATATTGAAAATAATAAGGGTAAACAAGTTCTATATGATGGAAGAACTGGTGAACCATTCGATGGTTTAATTTCAGTTGGATTAACATACATGTTAAAACTAGATCATATGGTTGATGATAAAATTCACAGTCGTTCGGTTGGTCCATATAGTAAAATTACACAACAACCATTGGGTGGTAAATCACAAAATGGAGGCCAACGTTTTGGTGAAATGGAAGTTTGAGCTTTAGAAGCTTATGGTGCAGCTTATAATTTATTAGAAATTTTAACAATTAAATCCGATGATGTTCAAGGACGTAATCAAGCTTACAATGCTATTATAAAAGGTCACGATGTTGTTGCTGATGGAATGCCAGAATCATTTAAATTATTAACAAAACAAATGCAAGGTTTAGGGTTATGTATTACCGTTGAAACAAAAGATGATCGTATGGTAGATATTAATGAATATACACTAAATCAAAATCGTTTAAATAATGACGATGATGAGGTTATTTTAGATGAAAATCTAAAAGAGATCAATGATTCTAATGAAGAAATATTTAATACAAACTTTAATAATAATGACTATGATGATGAAGAGAACTTCTAAATAATAGAAAGGTAAAATAATATGAGTCAAAAAGGGATTAAATCATTAACGATTTCCATTGCTTCACCTGAACAAATTTTAAATTGATCAAAAGGTGAAATCACTAAACCTGAAACAATTAATTACAAATCATTAAAACCAGAACCTAATGGGTTGTTTGATGAAAGTATTTTTGGGCCTTCAAAAGATTATGAATGTTATTGTGGAAAATATCGTAAAGTAAAACATAAAGGTAAAATTTGTGAACGTTGTCATGTTGAAATTACTGAATCAATTGTACGTCGTGAACGAATGGGACATATTGAGTTAGCTGCTCCTGTTGCCCATATTTGATTTACAAAAGAATTACCTTCGCCATCAAAAATCTCATTATTATTAGATATTACATATAAAGAAGTTGATCAGGTAGTTTATTTTGTTAATTACATTGTTTTAGATGAAGGAAATAATGTATATGATGGTAAATCAATTTTTAATAAAAAAGAAGTTTTAGATTTAACATCACCAAAAAATTCAATTCGATCACGTAATAAATTACGTCGAACATTACGTAATATTCAAGAACGAATTGAAGATGAATTAAATCATGAACGTGAAGCATTAATTCAAGATTTTGATTATCGTTTAGCTGTAACATATGATCAAATGTTAAAAGATTCTAATATTCCTTTTTCAGTGAAAGATGTAATGGCTTTTATTGAAAAACATACAGGCGTTCGTTTTGGTATTGGTGCTGAAGCTATTCGTGAATTATTAGAAAAACTAAATCTTGAAGAAGAACATGAAAAGATTAAACAAGCAATTCAAAATTCACCTAATGCTTATGATCAAAAAACAAAACGCTTATTGCGTCGTTTAGAATGTGTAAGATGAATTAAGGATTCAGGTTCAAAACCAGAATGAATGGTAATGACAAGAATCCCTGTCACACCATCTGAAACAAGACCAATTATTTCTTTAGATGGCGGTCGTTTTACGACCTCTGACACAAATAATTTTTATCGTAAAATCATTATTCGTAATGAACGTTTAAAACAAATGCAAGCAACAGATGCTCCTGAAATTTTATTAGATAATGAAAAACGTTTATTACAAGAAGCAGTTGATTCATTATTTGATAATAATTCACGTAAAAAACCAGTTGTAGGTAAAGATAAACGTCCTTTAAAATCATTGTCAAATCATCTTAAAGGAAAACAAGGATTATTTAGACAAAATTTATTAGGAAAACGTGTTGACTATTCAGGTCGTTCTGTAATTGTTGTTGGTCCTGAATTAAAAATGTATGAAGTAGGAATTCCTGCTTTAATGATTTTAAAATTATTTAGACCATATATTATTTCTGAATTAATACGTAAACGTGATGAATTAGGTAATGAAATTCAACCAATTTGTGCCAATATTAAATTAGCAGAACAAAAAATTCTTGCGCAAGATAATGAGATTTGACCCGTTGTTGAAAAAGTTATTAAGCAACGTCCTGTAATTTTAAACCGTGCACCAACCTTACACCGTTTAGGGATCCAAGCTTTTGAACCAAAAATGGTGGACGGTAAAGCTATTCGTTTACATCCTCTAGTTACAACCGCTTTTAATGCGGATTTTGACGGAGACCAAATGGCTGTTCATATCCCCTTATCCAAAGAAGCAGTGGCTGAAGCACGATCAATTTTATTAGCCTCATGACATATTCTTGGTCCAAAAGATGGGAAACCTATCATTACACCCACACAGGATATGATTTTAGGTATTTATTATTTAACAAAAGAAAAATTTCCACAAGTAATTGAAGAAATGATGGCTAAAGATCCAACGCAAGCGAGAGTTGAATTTATTAATAATTTTCACATTTTTTCAACACAAGACGAAGCTATTCGTGCTTATAAATTAAAAACAATTCGTATTAATGACGTAATTGGAATTACAACAAAAGCTTTTAACAATAAAACATTTAGTAAAGAAGGTATTTTGGTTACAACGGTTGGAAAAATTATCTTCAACCAAGCTTTTCCTGTTAATTTTCCATATATTAATGATGTTAAAAATCTATATGGCGAAAATCAATTTGAAATTATTGGAATGCATGAAAGTATTTTAGATTATTTAAAAGCCTATAATTTAAAAGAACCATTAACTAAAAAAACTTTATCAACAGTAATTGATTATTTATATAAAGTTTCTGAAATCGAAGTCGTACCACAAACAATGGATAAAATTAAAGCTTTAGGATTTAAATATTCAATGATTTCAGCGACAAGCATTTCTGCCTTTGATATTCCATCTTATGATCAAAAATATGAATATTTTAAAGAAACTGATGAATTGGTTTCAAAACTTCGTGAATTCTATTTAGATGGTAAATTAACTGATGATGAGCGATATACTAAAGTTGTTCAAGCTTGATCACAAACAAAAGATAAAGTTACTCATGATATTGAAAAACTAATTAATTCTAATGAATATAAAGATAATCCGATTGTGATTATGGCAAAATCAGGAGCTCGTGGAAACACGTCAAACTTTACACAGTTAGCAGGAATGCGTGGTTTAATGTCAAAATCATATAACTATGACCAAAAAAATAATAATGGTGTTATTAAAGATACAATTGAAATTCCAATTAAGCATTCATTTATTGAAGGATTATCAGTTAGTGAATACTTTAATTCATCATTTGGAGCACGTAAAGGGATGACTGATACGGCGATGAAAACCGCAAAATCAGGTTATATGACACGTAAGTTAGTTGATTCAACACAGGCTGTTGTAATTAAAGATCATGATTGTGGCACAAAAGAAGGTATTATTGTTCGTGAAATTCGAAATACAAAAGATAATACATCAATTGAATCACTAAAAGATCGTATTGTTGGAAGATATAGTATTAATACAATTTATGATACAAAAAATAAATTAATTATCGAAAGTGATAAATTAATTACAAGCGAAATAGCTAATATCATTCAAAATTCAGGTATTCGTGAAGTTGAGGTTCGTTCTCCATTACATTGTGCATCACTTTATGGTGTTTGTCAAAAATGTTTTGGTTTAGATCTATCAACAAATAAATTAATTGAAACCGGTACAGCGATTGGAGTAATTGCAGCGCAATCAATTGGTGAACCTGGAACACAGTTAACAATGCGTACTTTCCATACTGGAGGGGTTGCTGGTGATACGAATATTACTCAAGGGTTTGAAAGAATTAAACAATTATTTGATTGCATTCAACCACAAGAAAATGAGAAAGCAGTTATTTCACAAGTAAAAGGAACTGTTGAACGAATCGAAAAAGATTCAAACACCAATGGTTATAATGTTGTAATTAAATATAATAAAGATAATTATGTAAATTATCCAACACGTTCGAATGCTGTATTACGTGTTAAAACAGGAGATGAAATTATTGCTGGTCAAAAAATTACTGAAGGATCAATTGATGTTAATGATTTACTAAAATATGCAGGTATTGAAAATGTGCGTCATTACATTATTAAAGAAGTTCAAAAAGTTTATCGTATGCAAGGAATTGAGATTTCAGATAAGTATATTGAAGTAATCATTTCACAACTTACAAATAAAATTACAATTACAAATCCAGGAGATTCAGGATTATTTGTTGGAGAAACAATTAGTATTAATGAATTTACTGAAGTGGCTCAAAATATGCTTGTTAATAAGAAAAAACCACCAAGTGCAATTAATCAAGTATTTGGTTTAGACCATGCGCCAAGTAAATCAGGTTCATTCTTATCTGCTGCTTCGTTCCAAGATACTAAGAAAATATTAACTGACGCTGCTGCGCGTTCACAAAAAGATATGTTAATCGGCTTGAAAGAAAACGTTATTCTAGGAAATTTAATTCCTGCTGGAACAGGATTAAAGGATGTTGAAGAAGTGATTGCTTATGGTGAAGAAATGTATAAAAAGCAATACTAAATCCTTTTATTAAAAGTTTCACAAAATAACTTTGATATTTACGTAGATGTGTGCTAAACACATTTACGTTTTATTTATATATTTTTAATCATACTTATTTTTAGAAAATAGGAGAAAACAAGATGAAATTATTAAAAAACAAAAAAATTTGGGTATTAACAATTAGTAGTGTTTTAATTGGTGTAAGTGTTATTACATCAATTGCTTCATGCTCTAGTTCAAACATAGAATCTAAATTAAGTGATAAATTAGTTGAAGATGAAGATAATAATTATTACGTAGCATATCAAATTTCAAATTATGAAAAATTAAAAGATTTTGAAAAAAAACAATTAGAAAAAGTCACTTTTTCAACATCTATTACCCCAACTGATAAAACAAAAAACTCACAAGTTTTTAATGCAAAAGTAAATGAAGGCATTTTATTCAATCAGCAAGCGAAAACATTTTATATCAAATTAGTTCGTAAGCCTGAAGTTGGTGAAAGTATTATTATTTTACCAAATAATTCAATTTTAAAAACACACGGCTTTGTAGTTACAAATGAAAATTTAAATTTTATTAAAATTAATAAAAAAGGCGAATTACAAAGATCTAATACGGCAATAACTAAAAAAAATAATTTAACTAACGAATATAAATAGAATTAAAATAAATAATGATTAAATAAAAAATCTGATGGTTAACAGACTATCAGATCTTTTATTTAATAAGAGTCTATTTAGCTTCAATTAAGATAGCAAAATTATTTAAACCCACATGCGCATAAATACAACCCGGTAAATATGAAATTTTAATTTCAATAGTTTGTCGAATTTTTTCTTGTAATGAAGTTTTTAATGAATTGATTTGTGATTCATTTTCTAAATCAGTAAGAATTGCAATGTTCTTAATACCATTTGTGCGCCAATGATTTTGATTATCAATTATATCTAATAATTTATCTATAGCTGTTGATAAATTCGGTGTTTTATCCATAAAATCTAATTGCCCATTTCAACGAACAATTAATTTTAAGCGTAAAGCTTTAGCTATAATTCCTTTAGTTTTTTTAACACGTCCGCCCGCAATTAATTGGTCTAAATTATTTACGATAACTCCACCTATAATTTTTTGTGAACGTTGTTTTACTAAACTAATTAATTCTTCATCACTCGGATTATTTTTTAAGAGTAAATTAATCTCATCAACTAATCATAAAATACCAATTGAAACAGAACGTGAATCAATGCACACTAAATGTTTGTCTTTGTAATCTTCTTTAATAATACTTAGAGATTCAAAATAACTTGATAAACCACTTGATAATGTTAAAATAACAACACGATCATATTTTAAGAATAATTCATCTAAATTTTTTCATAACAAAACCTGGTGAAGGTAATGAAGTATGAATATTTGTTTTATTAATTAATATTTGTTTCAATTGTTCTTGACTAACGTCAATAACATCTTTTAATTCTTTTTCATATTTGTTGTTCTCTAATAATTAACTGAATAGGCACAAAATATACATTATCATCTTTAATATCTAAGACGTTAGCACCTGTATCGATTAAAATTGCTGTTTTCATAAATCTACCCCTAATCAACTAAAACTTATTAAAAATTATAATAAAAACAACATTATTAACTATGATTAAAAGTTAGATAAAGTTAATTTAATATTATAATTTATAAATAAAGAGAGTAATTTCTCTTTAAAAATTGGAGTTGAAATGTATATTTATTATAATAAAACAAGTCTAAACGATACGTTAATTTTTTTAAAAAACAATCAGCAATATGATCATCAAGTGTTTTTAAACGATGACTTATTGCTTTTTTATCATGGTGATGAACTAATTGGTTTAAATTTATTTAATGCAAGTGAATATTTAAAAAATTTAAATGAAGGATATTTATATCCTACGACTCAATTAATTCAAAAATTAAGTGATTTAATAAAAATAAAATTATCATTAGATAATGCTTTTAAAGGTTTTGTGGTAGGTAAAATTTTAGAAGTTAATTTAATTCCCAATACTCATTTACATATTTGCTTAGTAGATATAGGTGATCAACAAATACAAATAATATGTGGAGCACAAAATGTCCGTATTGGCTTAAAAACGGTTGTAGCGACACCTAATTTATTAATGCCAAATGGAAATGAAATTAAAAAATCCAAATTAATGAACTATGATTCATTTGGAATGTTGTGTTCACAAAAAGAATTAAATATTAATAATTTTAATTCACAAGGAATTGTCGAACTTAATGATGAATATCAGGTTGGTCAATTGTTTACAAAAGTTTATAGTAATTTATAATTTAAAGTAGGTAAAATTAATGTCAAAAAATGATGATATATTATTATTTAGTCTTTCAAATTCACATCAATTAGCTAATAAAATTGCTAATTTACTAAAAATTGAATTATCACCAATTAGAATTGATAAGTTTGCAGATGGTGAATTAATTGTTGCACCTCAAGTTCCAGTGCGTGGACGTCGAGTTATTATTATTCAATCAACATCTAAACCTGTTAACGATAGTTTAATGGAATTATTAATCGCTATTGACTCAATTAAACGAGCTAGTGCCAAAGCAATTAGTGTTGTAATTCCTTATTATGGATATGCACGTCAAGACCGTAAAGCTAAACCACGTGAACCAATTACAGCACGTTTAGTTGCAAAAATGATTGAGAGTGCGGGCGCAACTAGTGTATTAACTTGAGATATTCATTCTTTACAAACACAAGGTTTTTTTGATATTCCGTTTGATTCATTAGAGGCTGTATGAGTTTTAATGAAACATTATTTTAGTGCTTATAAAGATTCATCAAATATAACTATTGTTTCACCAGATTATGGTGGTGTCAAACGTGCTCGTGAAATTTCAATAGCTACAGGTGCAACATTAGCAATTGTTGATAAACGAAGATCTGGCAAAAATCAGGTGGAAATTAATAATGTATTAGGTGATGTTAAAGATCGTGATTGTGTAATTGTTGATGATATGATTGATACTGGTGGCACAATTTTAGGTGCTGCTAAAATTGTGCGTGAAAAAGGCGCTAAATCAATAACAATTATTGCTACACATGGTTTGTTTAATAATAATGCACGACAACATTTTCAACAAGCAATTAAAGATCGAATTATTAATAAAATTTGTATAGCTGATACAATTGAAAATGAACCTTTTGATGGTCTTGAAATTGTAAGTATCGCTCCAGCAATTGCCAAATGTATTGAAATTTATTCAGAGGGTACAGGTTCGATGTCGTTTGTACATGATGAAAATTCAAAAGTTTTATTTGCTAAAAAAATATATTAAATAAAAATGAATAAACCAATTATCAAAGAAGTTATTATTGTAGAAGGTAAAACAGATGCACAAAAAATTGATCAATTAGTAAATGCACAAATTATTACTACTAATGGATCAGAAATTAATAAAAAGACTTTAGCTTTAATTAAACAAGCACAATTATCAAAAGGAATTATTTTATTTTTAGATCCTGATTATCAAGGAGAAAGAATTCGTAAAATAATTACTAATTATTTAAAAGATGGCATTATTAAACAGTGTTTTATTAGTAAGGACAATATGAAGAATAATTCTAAAAAAATTGGAATTGCTGAAGCTAATAATGATGCTTTATTGAAAGCTTTGAAATCTCAAGGAACATTCGTTATTAATTCAATTGAATCTATTACTTGATTAGAATACTTAAGTTTAAATTTAAATAACAAAAAAATACGTTTAATGCTTTGTGATTATTTAAATATTAGTTATTGTAATCACAAACAATTATTTAAAAGATTAAACATGATGCAAAAAACATTTAAAGAAATTAAAATAATTATTGAAGGATTTTAACTTATGAAATTATGTGATAAAGAATATATTTTATCTGTTATTGCTACACCAATTGGCAATCTTGAAGAAGTTAGTGAACGTGTTATTAAATGTTTAAATGAAGCTTATGTTATTTTATGTGAAGACACTCGAGTTACAGCTAAGCTGTTGCATCTATTAAATATTTATGATTACAAAAAATTAATTAGTTATCATAATTTTAATGAAATTCAAAAATTGAATGAAGCGATTAATTATATTAAAAAATATCCAACAGCTTTAGTTAGTGATGCAGGCTATCCAACAATTTCAGATCCTGGTTATAAATTAATTAATGAATGTCATCAACAAAATATTGGAATTCAAGTTATTAATGGTCCCTCAAGTTTGATGCATGCTCTAGTTGCTAGTGGAATGTGTTCGCAAGATTTTATGTTTTTAGGTTTTTTAGGTAAAACGCAAAAACAACGTGTTGAAAAACTAAAATTATATAAAAACTTGCAAACAACTTTTGTTATTTATGAAGCTGTGCACCGAATAGAAAAGACTTTAATGGATATATATGAAATATGAGGAGATATTTTAATTTTTATCGGGCGCGAACTAACAAAAAAAAATGAAAGTCATTATTATGGTTATCTTTCACAACTACCCCCAATTACTGAAAAAGGAGAGTTTGTAATTGTTATTGATAATAAGATCAATCAAGATGTTATTAAGAATAATAATATTAATGAATGTCTAATTAAAATTAGAGATTTAATGAAACAAAATTTTAAATTAAAAGATGCTTGTAAAGAAGTTAGTAAATCTTCTAATTTTGATAGTAAAGAATTATATAAATTAATAATTAATCAAAAATAATATGTTAGATAAATTAATTAAAACTCTAAAAAATCCTCATAGTATTGATGACATTGTTGGTCAAAAATATTTATTAGATGAAATTGGTATAATTAGAAGAATGGTTAATCACCATCAAGTATTTAATTTAATTTTTTATGGCCCCCCAGGTGTAGGAAAATCATCGCTTGCTAAAGTTTTAGCACAAGATTTAAAAGTTCCATTTGCTTTTTTCAATCCAGTTGTTGATTCAAAAAAGCAATTAATGCAAATCATTGAACAAGCATTAGATTTAAATAACTTTATTATCATTATTGATGAAATTCATCGATTAAATAAAGATAAACAAGATATTTTATTACCAATTATTGAAGCAAATAAAATTAAATTATTTGCGACAACAACTGAAAATCCGTTTTTTGTTATTAACCCTGCTTTAAGAAGTCGTTGTCAACTATTATCATTAAAACCATTAACGAGTTCACAAATTAGTGAACGAATTAAAACTTTGATTAATGTTGAAGAAGTTTTTGAACCACAAGCTTTTAATTTATTAATTAATAAAACTAATGGAGATTTAAGGGCTGTTATCAATACATTAGAAATTATTTATTTATTATATAAAGAACATAAAGTTGATTATGTATTACTAAAACGTATTATGCAAGAATCATACGCATTAGGTAGTAGCGATGGCGATGAAATTCATGACTTAAAATCAGCTTTTCATAAATCAATGCGTGGAAGTGATTGTGATGCAGCTTTATATTATTTAGCACGTTTAATAATCATAGGTGATTTTGAAGCGATTTATCGAAGAGTTAGTGTTTGTGTTCATGAAGATATAGGTTTAGCTAATCCTAATCTATGTTTACGTGTTGATCAAGGAATTAATTCTTGTCGATTTTTAGGATATCCAGAAAATCAAGTAATTTTAGCTAATTTAGTTTTACAAATTTGTTTATCAAATAAATCAAACTCTGTATATCTTGCCTATCAAAAAGCATTTGAAGATGCTGCTAATGGCAAACAATATTCAATTCCAAATCATGTCCGTGATGCTCATTATGCATCAGCAATAAAATTGGGTGTTCATGGTTATAAATATCCACACGATTTTAAAAATAACTATGTTAATCAACAATATTTACCACGTGAATTAACAAACAAAAAATATTATGTTCCCCAAAACAACGAACAAGAGTTTAAATTAAACCAAAATCAACAAAAATTTAAAAATAATAATTAAAAATTTTAAGGAGAAAAATATGTTAAAACAAAAGAAATTTTTTATCTCTACTCCCATTTACTATTCTTCTGGAAATCCTCATATTGGTCATGCTTATACAACGATCATTGCTGATGTTTTAGCACGTTATAAACGCTTGTTTGGATATGATGTTTTCTTTTTAACAGGAATGGATGAGCATGGTCAAAAAATCCAACAAAAAGCTTTTGAAGAAAACATTTCTCCCAAAGCTTTGGTCGATCGTAATTCAATTATTTTTTTAAATTTATGAAAACGATTAAATATTAGTTTTTCAAAATTTATTCGTACAACGCAAATGGATCATGAAGAGTCTGTACAAAAAGTTTTTAGTTATTTATATAAACAAGGAAAAATTTATTTAGGCCAATGGACAGGTTATTATTGTGTATCATGCGAAGAAAATTATAATCCTGCTGAAATTATTAAAAGTCAAGATAATATAATGTTATGTCGCATGGGTCATAAATTAGAAACTAAGTCTGAAGAATCATATTTTTATAAAATGAGTGATCAAGCACCATTTTTAAAAACTTACTATCAAAATCATCCGAATTTCATTATTCCAAATGAACGTGCAAATGAAATGGTTAATAACTTCTTAAATAATTTAGAAGATTTAAGTATTTCACGTACTACTTTTGATTGAGGGATTCCTATTGCTGAAAATCCAAAACATGTAATTTATGTTTGATTAGATGCATTAATGAATTATTTAACTGCAACTGGTTATTTATCAAATAATGAGGAATTATTTCAAAAATATTGATGTGATAATGAAACTGAAATTGTTCATTTATTATCAAAGGAAATTGCGCGTTTTCACTGCATTTATTGACCAATCTTTTTAAATGATTTACAAATTCGTTTTCCATCAACAATTTTATCACATGGATGAATCATTACAAAAGAAGGCAAAATGTCTAAATCATTAGGCAATGTAATTGATCCTAATGTTTTAATTGATACTTATGGTGTGGATGCACTTCGTTATTATTTAATGGCTGATTTAAGTTTATTTAGAGATGCTATTTTTAGTGAAGATAATTTGATTGAAACTTATAATACTCAATTAGCAAATAGTTATGGTAACATGATTTCAAGAACATTGGGAATGTTAAAAAAATATCGAAATAATATAGTTCCAAAATATGTTGGTTGTGTTTTAAAAAATGATGAAAAATTAGAAAATTTAATTAATAAAAATATTGAATTAGTTCAAGAAAACATTAATAAATATAGTATTGATAAAGCACTTAATTGCATTCAAGAAATTTTAGTTGAAGCAAATAAATATATTGAAGATAATAAACCTTGAGAATTAGCTAAAAATCAACAAGAGCAAGAATTAGATTCACTTTTAGTTCATTTAGTAAAAGTTATTCAAGTAACTACTACTTTATTATCACCAATTTTAATTGAAGGAAGTAAAAAAGCAGTTGAACAATTAAATTTTGATGAATCTTTTTTAACTTTAGCTAGTTTAGCATCTTATGATATATTCAACTACCATAAAGTTAATGATTCAAAACCAATTTTTGCAAGAATCATTGTAGAAAAACAATAAAATCTTTAATTCTTTGTAATTATTAAAATAATTAAGCTTTTTTTATATAATTTATAAGACTAACATTTAATAGTAGTTGAGGAATTAATGAGGTTAAGATATAAAATCTTATGCGCTAGCTTAGGAGTGGTTGGTGTTGTTGGTGTTATTGCTGCAATATCAATTGATTATAAACAAATTCCAATTGTTTATGCAAATGGTAGTTCATCAGTATTACCCTTAATTCAGGCCTTATCTGAAAAACATCATAAACAAGATATTATTTCTAATGCTGGAGGTTCTTCGTTAGGAATAATTGAAGCTGTTGAACAAAGAACTAATATAGGCGTAAGTTCACGTAGTCCAAATATTGATTTTGAGCAACAAAGTGTTGAAAAAAGTCATGTAGTTTATGATAAGTGAAAACAAAATCAATTGAAAACTTATACAATTGCTTGAGATGGTATAGGCATTATTTATAAACTCAATAAAACTAATCATGAATTAGTTTTAACAAAAGACAATATTATTAAATTGTTTAAAGCTTTTACTGGGAATGAACAAGTAAGTTATTATGATCTTGATCCCCAATTACCAAAAACACCAATTTTAGCTTATTCACGCACAGGTGGAGCAAATGCCTCAGGGACAGCCGAGGCTTTTTTGACTTCTAATCCATTAATTTCTAAAAAAGAATTTAAAAAAAAATATCATGATATTTATAAGGTTATTGAAGCTGGTACTTATGGCAAATTAATAAGAAGTACTGAAGAATCGAATACTCAAGCTTGAACAAATATTTCTAAAGATAATGTAGATGGATCAATAACTTATTTATCAACAGGTTTTATTCTTAAAAATTATGATCAAATTATTGCTAAAGGATATAAGATTGCTTATTTACAAAATAAAGATTTAAAAAATTATTTACCTTTTGAAATTAATGAAAACAAACAAGAAATTGTTAATGTTGCTAAAACTTATGGATGATATCGACCTTTTAATTTAGTTTTTAGTTTAAATATGCAAGGAATTAAAAATACTAAAGAATTCGTTTGATGATTATTAACCAATAAAAAAGTTGAAAAAATCATTCAAGAATCAGGGTTTATTCCCCTTACTTTTGAACAAAAAAATTCAATGTTCTTAAAACCTTATAAAGATGAACAATCTTTTCAAGAAGCCATTAACAATCAAAGCGCAAAAAACAATTTTTTTGTAAGTGATTTTGAATTAATAGAATCAAATAGAATTAATATATTAGGAGCAAAATCAGCATAATGAGTATTTTAAATCTTAATTCGCAACAGACTAAAAAAATCATTTTTAACAAAAAAATAAAAGATAAACTAGCTAAAACGTTAGTTATTAGTTTTGCTTGATTATTTTCTATTTGTTTTATTGGTTTAGTTGTTTTTATTATTATTCGTTCAATACCTGGCTTTGAAGCTTATGGATTAAAAAACATTTTTTGAAGCAAGAATTTTAACTTAGCTGATTATGCTGCTGGTTCATCAGTTTGATTTCCATTAGCAATTACTTTGCTTGTTTCGTTTGGTGCAATTATAATTGCTGCTCCAATTGGTATTAAAACAGCTACTTTTATTAAGTTTAGAATTAAAAACAAAAGATTACAAAAATTTTTTAGAGTCACGATTTTAGCATTAGCTGGAATTCCTTCAGTTATTTTTGGATTATTTGCTATTCAGTCTTTAGGTCCTGCAATTTCGTTTGTTTTCCACATTGATACTGTTCAAAACATTACTACGTCAATGTTTATGTTAGCGTTTATTATTATTCCGACAATTATTTCTTTAACGCTAAGCACATATGATGGTATTGATATGCGTTTAATTGAAAATGGGATAGGAATGGGAAGTTCTTATACACGTTCAATTTATAAGATTTTTAAAAAAGAAGCTCGTGGTGGTATCATTATTGCCATTATTATTGCCTTAGGTCGTGCGATCGGCGAAACAATGGCCATTAGTATGATTTTAAGTGATCAAGGTTACCAAAACATTTTTGGTACTGGTTTTTTACAAATTATGCACTCTGCTCTTCGTCCATTAGGTGCTGTAATTTCAGCTAACATGTTCGCTGAAAATGGTGGCGAAGGTTTAAGAGGATTACTATATATTTATGGGATCGTTTTATTTGTAGCGATTATGATTTTAAATGGCCTTGTAACTTATTTAACAAGAAAACGTTCAAAGAAAACATATGCTTGATTTATTAAATTAGAAAAAAGTTTAGCTTATATAGTTTGTTTTATACCAGATCAATTAAAAATTTTATATGAGAAAATTACGCATCGTTCCCAATATAAATTACATGTAAACAATTTGGATAACTTAAATAACTATATTACAGATCGTATTCAAAATCGAAAACTAAAACGTTTATATACTGTTCACAAATTATTTTTTGAATCATTAGCGTTTATGGTTGCTTTTGCTTTTTTAGCTTGAATTTCACTAGATATTTTAGTTAATGGAATTAAAGCAATTAATTTACCAACATCAACAGTAGTAGCTTATACAAAAAACACAACTGGACAAGCAACAATTAATACTTTAATAATTATTATTGTTGCAATTTTAATTGGTTTACCATTTTCATTGTTTGTCGCAATTTATATTAATGAATATGCTAAAAATAAATGACCAAAAAAAGTTTTATTATTTTTTATTGATTCATTTGGATCAACGCCTTCGATTATTTTTGGGATGTTTGGTTTAGTTATTTTTATTGAAATTTTTGGTTTTACTTCAATGGGTAATATTGGTAAATCACTATTAGCGGGTGCTTTAACAATTACTTTAGTAGTCCTTCCAACATTTACACGTTCAATTCAACAATCTTTAAAAGCAGTACCAATGAGCATTCGTGAAAATGCTTATGGATTGGGATGTTCAAAGTGAGAAACAATTGTTAAATTAGTTTTACCACAAGCAAAAAAAGGAATTATTTCTGCGATTGTATTAACAATTGGTCGAATTGTAGCTGAAACTGCACCATTGTATTTAACAGCAGGTTTATCATCAGCTCAAAGCATAACAATTTTAAATCCTGGACAAACATTAACAACACGTATTTATGCTCAATTATATGAAAATAATACAAATATTGCTCATAATGTAATGTACGAATCTGCTTTTATTACTTTATGATTAGTTATTGCATTAATCATCATTGCTCACGTTATTGTTCCTTATTTTGAATTTATTAAACATGATATTAAAAAATGGTTTAAAATTTTAAAAAATTATCTTCGCGCACCTTACATGCGTGATATTAAAGTTTTCAAACCACAAATTTATCAAAAACATCTTTATTTAACACATAATCAAGCCAAAATCATGGGATATAATGAATCAGTAAATAAAGTTGCTAAAATTGGTTTAAAGTTATATGAAATTCGTTATGTTGATGATGAACAAATTCAAAAAGAGTTATTAAAAGTTAGGGGATAAAAATATGAATGATCAAAAAGACAATAAAGAAATTCAAACAATTGTTCCTTTAAATCAAAAAACTAATTTAAATGAATTGGATGCTAAAGCAATTTATGATGAATTTCAAAAATTAAATAAATACCAAAGAGCTTTTGCTTGACTAAAACTTCCAAAACAAAAAAAAGCTGAATTAAAGCAATTTTTAAATAAGAAAAAAACGCAAGTTGATTTGCTAAAAGAAGATTTTAATCACGCAAATGTTTTTGAAATTCGAAACTTTAATTTTTGATATATGAATCGAACAAAACATGTTCTACATGATTTAAATTTGGATATTAAAAGAAATAAAGTAACAGCATTTATTGGCCCAAGTGGTTGTGGTAAATCAACTTTTTTAAGAAATTTAAATCAATTAAACGATTTAATAGAAGGGACTAGTCACGAAGGTGAAATTTATTTTTTAGGAACTAATACACGTTCAAAAAAAATATCATCATTAGAATTAAGAACACGAATCGGGATGGTTTTTCAAAAACCAACACCATTTGAAATGAGTATTTTTGATAATGTTGCATATGGTCCACGAAATAATGGAATTAATGATCGTAAAATTTTAGAAAAAATTGTTGAAAAATCATTAAAAAGTGCAGCTTTATGAGATGAAGTTAAAGATGATTTAGATAAAGCTGGGAACGCTTTATCAGGTGGTCAGCAACAACGTTTATGTATTGCGCGAGCAATTGCTTTAGAGCCAGAAGTGTTGTTAATGGATGAACCAACAAGTGCTTTAGATCCAATTGCAACAGCTAAAATTGAAGAATTAATATTAGAATTAAAAAAGAAATATTCTATTATTATTGTGACTCACTCAATGGCACAAGCCCAGCGAATAAGTGATGAAACTGTGTTTTTTTATCAAGGGTGAATTGAAGAAGCGGGAGAAACAAAAACTATTTTTATCCATCCTAAAAATAAAAGAACAAAAGATTATATTTCTGGAAAGATTGGTTAATAAAAAGAACTGGTGATATATTTATGGCTACTAATTATTTTTTAATGAAAGAATCACAATGTGATTTACTAAATGATTTTAAAAACTTTTTTCAAATGGTAATTGATAATCAAAAACTAATTTGTGATTTATTAAAAAACGATCAATCATCATATAAAGAAACATATGAAAAAGCTTGTTTTTTAGAAAAAAAAGTTAATACAACATATGCTGATATGTTAGAAGAAATTATGTGAATTATTCAACGTGACCAACCACGTGCTAGTTATTTACGTTTTTTTATTGCAGGAATCAATTCTATTAAAGATTTAGAAAGAATTTCTGATCACTCTGAAATTATTTGTGAATACTTTTTAGAAACTGAATTTGATGAAGAACAAAAAAATTTTTTTTTAACTAGCTTTGAGATTTCTAATCAAATTCTTTCTGATTTATATGATTTTTTTGATAAAAATGAATTTAGTGTCAATACAATTGAACATATTAAAGATTTACGTTCTAAATCAATTATGAAAATCAATAAAAATTTAATTCAGTCCTTACATGATCAATCAAAACTATTAGATGAAAACTCAAATTATATTAAATTAATTTTAATGTATCGTCATATTGAACGAAATATTGAACATGGCATAAATATTGTGCAGAACTTTGGTAATGTGCACATTACCAGTCGTTAATAAATCATTAGCAAAAACTAGGCATTTACCTAGTTTTTTTATTTATATAAAAAATAAGTCTTCCAAAAATTTTACATAAAAAGTGAAAAAAATGGAACAAATTTATATTTTTGTGTTTTAATATTAGTGTACCAATTATTTTACTTGGAGGTCTTATTTATGACTTATATGCTATTAACATCTAGTGACTTACATTGCGGGTCATGTTCTTCAAATTTATATAATGTACTTGAAAAGATTGGCGCACAAAATATTAGTGTAAATATTTTAAATTCAGAATTTGCTTTTGAATTTGAAGAAAATAAAATTGCTGATCAAGATGTAATTAAAGAAATCAATAAAAATGGTTTCAAAACAAATATTTTAGAAAAATACATATACTAAAAAATGTTAAAGAATAATTTAATTAAAAAATTTCAATTACTACCTATTAATAAAAGAAGATATTTAATTTCTTTAATGAAAACATCAATAGCACTTCTAATTAGTATTCCTTTAATGGTTTTTGAAATGCTATTTATGTTTAAAAGTAATTTAATTTTAAGCATTGATGGTTATTTTATATATGGATGAATTGTTTTTGTTTTAAGTATTTTCATTGTCTTTGGTTTAGGTTTTAGTTTTTTTAAGGGTGCTTTTTTTGAAGTTTTTAAATGAAAAAAACCTGGAATGTCTTTACTTGTTGTTATTTCAACATGTGTTGCTTTCATATATAGTACTTATAGTTTAATTTCTAACACAATTATTTATGAACCTAAACTACATGGTTTTTTTGAAACAGCTTGCATGATTATAGCGACAATGAGTGTTGGTCAATTAGTTAGTGATCGTATTAAACTAAAAGCAAATCAAGATCTACAATCTTTAAATAATTTACAAGTCAAAAAATATAATAGTTATGATTTGAACACAAAACAAGTTAGTGAAAAAGTTGTTTTTCAAGCTAAAATTAACGAATACGCACTAGTTAAAAAAGGTGAAATTGTTCCATTAGATGGCGTATTATATTCACAAATTGCTGAAGTTGATGAATCATCATTAACTGGTGAAGCTCGTCCAATTCTAAAAACAATTAATAATGATATTATTGCTGGATCAATTAATGTTGGAGATAATTTTATTTTTAAAATTACTAAACTTTATAATGATTCAACGATTAAAAAAATTATTAATGGTGTTAATCAAATTGCTAGTAGCAAGCCAAAAATTCAAGTTGTTGCTGATAAGATTTCGTTATGATTCACTCCATTTATCTTATTAATGGCCATTCTAGCTTTTTTACTACAAGCTTTTGTACCAAGTATTCAAGAGTTACCAATTGCTTTTTTAAATCTTCATGGATCAAATAATGATTCTAATTTATATGAAAAAGCAGCATATGTAGCAGTTTCTGTTCTAGTTATATCATGTCCATGTGCATTTGGTATTGCTGTGCCTTTAGCAGTTTTAATTGGTGCAGGGCATGGTGCTAAAAGTGGGATTACATTTAACAATAGTAATATTTTTGAAAAGATTAAAAAAGTTAATGCAATTGCTTTTGATAAAACAGGGACATTAACTTATGGAAAATTACAATTAAAACAAGTTATAGGAAATGATCAATTTTTAGATTTAATTTATCAAATGGAATTAATTTCTTTACATCCACTAGCAAAATCATTCGTAACTTATGCCATTATTAATAATATCGTCATGAGTACAAAATTAATTGATATTAAAGAAGTTGCAGGAGTGGGGATTATTGCTAAAGATGTTGATGGTAATATTTATGAATTAACATCAGAACATTATGCAAATGAAAATCAATTTGATTTTTCATTGATTAATCAAAAATCTTCTACTTCTACTAATTTATTAGCTTCAAATATTATTTTTAGTATTAACAAAAAGGTGCAATCAATTCTTGTTTTTGAAGATGAAATTCGTGCTGATGCTTATGAAACAATTAAGGTATTGCATGAAAATAATATTGAAACTTATATGATTACTGGTGATAGTTTTAATGTTGCTCAAAAAATCGCAAAGGAATTAGGAATTAAACATTTTTATGCTCAAGTTAAACCAGAAGAAAAAGCTAACATTATTAAAAAAATTCAAAATGATCAAAAAACTGTAATGTATGTTGGCGATGGTATTAATGATTTATTAGCTTTAAAACAAGCTAATGTTTCTATATCAATTGGTGAAACTAATAAAGCAACTAATGCAGTTGCTGATATTAGTTTAATCAAACCAGATATTTTAAATATTTACAAAGTAATTAAACTAACAAAAATTACTAAGATGTTTATTGTAAGTAGTTTACTATGAGCGTTTGGTTATAATCTAATTTTTATTCCACTAGCATTAATTGGTATTATTCCACCATTTATTTCTGTTTTAATTATGACAACTAGTGATATTGCTGTTGTATTAAACTCATTAATTTTTAGATTATTAAAAATGCGTCTAGTTAATAAAAAGCAAATTCATAAACTTAATTTAAAAATTATCAATGAGGCTATGTTACATAAATAATTTTATTTATTTTTTAATTTACTAATTAAATAAAAAAACACTTGTTGTATATTAAACAAGTGTTTTTGTTTTAATTAATTAATAAACTCATGCGAAGATGAAATAATAATGAATTTGGAGAACATTCCTTATAATAAGCGACAAATTATAAAGATAATAGGCATTTAGTTTAATAATTAATTTGCACTTCTTTTAAGAAAAGGACGAAATCTAAAAGAAACACACATTAAAATTATAAAATCTATTAATAAAAAAACAGCTTTTCATATATCTTTTAGGTTAATGATATTTATTTCCTATAATTAAAAAAACATTAGTTAATTAAATTTAACTAATGTTTTTTTATCCTCTTAAGTTTAATTCTTTAATTAAAGCACGATAACGTTCAATATCAACACGTTGTAAGTATGATAATAATTTTTTACGTTGAGCTACTTTTTTGTATAAACCACGCTTTGAAGCTTTATCTTTTTTATTTTCAATCATATGTGTTGTTAATGAATCAATTTCAGCACTTAAGATTGCTACTTGAACTTCTGTTTTTCCTGTATTTGATGCAGAACCACCAAATTTAACTGTTAAATCATGTTTTTGTTGTTTGCTTACTGCCATGATAATCTCCTTGAATTGTATATTTTTTTAATCTATTCCTAACTTAGTTTAATTTCGAAGATTTTAAATCAAACTATGCAAGAAATCAATATCTAATAATTTTAACATAATTATGCTTTTTTTGTATAATCATCAAGTTTTAAAATTGCATTTTTAGCTGCTAAAATTTCTGCTTCTTTTCGCGTTGATGCTATTCCAATTCCATAAATTAAATCATTTCAAACTAAATGGACTTCTTTTTTATCGCCTTCATTACGTACTAATTTATACATAATAGGTTTTTTATTAATACGTTGGGCTTGTTCTTGAAAAATCGTTTTATAATCTTTTTGATAATTAATTTGGCCTTCTCTAAAATATTTAATTAAAGTTTTTTCAAGAATTAATGAAACTTTTTTAATACCTTGATCTTGAGCTACAGCACCTATAAATGCTTCAAAAATATCTTCATAGATTTTTTCCATACGAGCTGATTGGTCTTGTTCTAAACCAACACCAATCATTAAAAAATCAGTTAACCCTAAATCTTTAGCAGCATATGATAAAATCTCGCTTCGTACCATATTAGAACGCGCTCGAGTCATTTCACCTTCATCTAAAGAACGCATTTTTTCATTATCTTTAATTTTGTAGTTAAAAATAAAATTGCTAATTACCCATTCAACACAAGCATCACCTAAAAATTCTAAACGTTGATAGTTTTTAGTAAGTTTATGCTCGTTTGCATAAGATTTATGTGTTAATGCTTCAAGATAAATAGATAAATTTTTTGGTTCAATTCTATTTTGTTTTAAAAAATCTAAAAATTTTTTATTGTCCATAATAATTATTTAAATCTTTTTTAATTTCATTTAAAACATCTGCTTTAATTGTTTCATACAAAATTTTTAAAGCACTCATAAATTGTTGGTAATCAGCACTACCATGGGTTTTAACTAAAATGCCATCTAATCCTAAAACAAAAGCTCCAGCATGTTCTTTATAATCAAATGCTTTTTTTATTTTTTTAAAAATTGGTTTTGACAATAAAGCAGCAAAAAGATTTTTTGGTTTTTTATATTCATTTTTTAAAAAATTAGAAATTGTTTTACCAACACCTTCCATTGTTTTTAAAGCAATGTTTCCACTAAAACCATCAGCAACTAAAACATCAACTTCTCGTTCTAAAATTGTTTTTGGTTCTACAAAACCCACATAATTTAAGTTTGATGTTTTTAATAATTCATTAGCTTCTTGATGATATGGTAAACCTTTATGATCTTCAGTACCAATATTTAAAACGCCAACACGAGGATTTGGAGTACGCATTTTAGCAATTGTATTAGCCATGATTGCAAAATTAAATAGATCTGTACCATCAACATCAATTGATGCACCCACATCTAATAAATTTGTAACACCACCATCAATTGTTGGAACAAAAGGCATAAAAGCAGGTTTCTTAATATGTTCTAATAATCCGATTGTAGCATAAGCATTATAAACAAAAACAGCAGAATTTCCAGCTGTTAACATTCCATCAGCTTGCTTATCTTTTAAATATCTTAAAGCTAAATGCATTGATGAATTTTGTTTTTTACGAGCTGATAAGATAGTATCATTTTGTTCAATGCTTACTTCAGTATGTACAATAGGGAATAAACGTCAATCATTATCTAATAATGGCTTAATTAATTGTTCATTTCCTACTAAGATAATTTCTAAATCATCTTCATGTTGATAATAAAAATCTTTAGCTGCTTTAATTACATGTTCTAAATCATTTTCATAACCCATTGTATCTAATAAAATTTTAATTTTTTCCATAATATTATTGAATTCCTATGTAGTAATGATATAAACTTTGACCAGTATATCTTAAATCACAAATAATTCCATATTTTTCTGAAATGTATTTTTCAATTTGATGTACATCTGTTTGTGAAGCATTAATGCCATAATATATATAACAAATTTCTGGTTTTTTTAAACTTTCAATTAACATTTCAATTGTTTTTAATAAAACATCATAAGCATTTTTTTCAGAAGCGATAATTTTTTTATTAATAATGCCTATATAATCATCTTTATTAACAGTAATATGTGGATATTTAACATTTTTAATTGAAGTAGTAATCATCCCACTACGTGCTTTTTTAACTATTTTATTCATTGTTTTAAAATTTGTGCGATAATCATTTTGCTGTTCAAAATGAGAAATCGCTAAAAGCGTTTCAAAAACATTATAAGCTCCAATTACAGAAACCAAAATGTTGTTTTCAGCAACGATTTTTGAAGCTTGTTCAGCAGCTAATACAATATTTGAATCATCAGTGACAATAAAAATTTGTGAAGAGTTTGTTGAGTTAATTTCATCGACAAAACGTTGAATTGATGGAGCTCCTTCTGTTTCAGTAACAATAACATTATCAATACCATGTTCTTCAATAAACATTTTTTTAAACTTTTCAGATGGAACTGTTGCAATAATTTTTTGCTCCATACTTAAAGTCAATTTATTTTCTTTTTTTGTAGTCAACCCTTTTCGTTCTAAACTTTCATAAAATTGGTTTGTCATGTTTTCAATTTTAATCTTATCAAATTCACCATATTTTTGTGATAATTGTAAGAATTTATAAGGAGTTAGGGTATGCAAATGAACTTTAACTAGATCTTCATCTTGGATTGCTACAATACTATCACCGATTTTTGCTAATTCACTTTTATATTTTTCAAATTCAAAAAAACTTTTACTTGGTGAATTAGGGATAATTTTTTTTCGTAAAGATAAAATAATTTCACTACAATAACCAAAACCTTCTTCTTTTTCTTTATGTATAATTTCAAGATCAAGCGGTTTAGTCTTTGATTTAATATTTTCTTCTTCGTTTTTAATAATTAATTTTTCATCAAATGTATTTGTTAAGCTTTGATACATTCCTTTTAGAAAACTTACTAAACCATATCCACCAGAATCAACAACATTAGCTTCTTTTAGCGAAGGTAACATTTCTGGTGTTCTTTCTAGAATGATTTGCGCTTCTTTTGTAACAAATGCAAATAAATCTTCGACACTATCAAAATTTTGACGTTCACTTATTCGCTCATTAATAACGCGAATGACTGTTAAAATTGTTCCTTCAATAGGTGTAGAGACGCTTGCATAAGCTTTTTCTTTTGCACCATTAAAAGCTTGTTTTAATTGTTCAATACTAACTTCATAAGCATCGTTATTTAAAGCATCCATAAATCCACGAAATATTTGACTTAAAATAACACCAGAGTTACCTCTGGCGTTCATTAATAGACCACGACTAAAAATTTTAGCAAGTTGGGAAAAATTGGATATTTCTATCTCTTTAATAGCAATAATTGCACCAGCTGTTGTGGTTTTCATGTTTGAACCAGTATCACCATCTGGAACAGGAAAAACATTTAAATCGTTAATATATTCACATTCAATTTCTAATATTTTTGCACCTGCATATAACATTTTTTGAAAAAGATCTAACTTAATTGTTTTCATATCACACTTCCATTTTAACAGTTTATAATGATGTTAATAACCACATTATCATCATTTAATTGTTTTGACAATTCATAATATACTAAATTACGCGCTTCATTTGCTACACTTAAAACATTAACAACATCTTCATGTAAATCAATTTTAATTACAAAATAATTACGATTAAAATCTGATAATTTGTCATTTATGTCAACACTAAAAATCCCAGGAACTTTTAAAATAGAGGATGTAATTAAACTTTTAACAAGTTGAGTATCAAATTTAGTTTTTTTTAACATGCCCTACTCCCACCACATTTACGCTCTCATATTTATAGTTTCAAAATTATATTATATTATAAAATTATTATTTATGTTTTTAGACAAATAAGATAAATACAATCTTAATACTTTTTCAAATTTTTTAAATGGTTTTTAACTTTTGTTGTTTTTAATAAATTTTGTAAATTTTCAAATTTATTAAAGGCCAAATCAATAATATCTTCATAATTATCTTTTGTTGCAGAATTTAAATAAAATTTTTTCAATACATTTTTATCAGCAAAAAGTAATAAACTATGATATTTTTGCTTTTTATTTCTTGAAATTTCAAATAGTAAAATTTGTGGATTGGAATTATTAATCAATTTTTTTATTGTATTATATATTAATTTATTTCACAATTCGTCTTTAAAATCGTTTTTTGTTAACGTAAAATTAAACTGGTTACTTAAATATCTGCTTAAATTATTTTTTTTAGTAACATAGATTTGAAATAAATTAATATAAATATCCTTATAAATAAAATTTTCTTTTAATAAGTCATTTTTATTGATCAAAATTTCATCTCAGTTTTTTCAACGACGCATTTTTGAAGGTAAATAAGCATTTTGACCAGATAAATAATATATATAATTATCATTTTCAAATAAAATAATAGGAAAACCTCCAATCATGTTTTCAAAAGGATCACTAACAATATAATTTAAATCACTATTTCTTATTAAAATCTCTTTATACATACTTTTTCAGTTGATATTCATAAAAAAATTTATTATATTACTATAAATATTTTATAATATTAAAATTAGTGAAATTAAAATATTTAAATAATTTTAACACGTGGAGGTGTTTAAAAAGATGGCAAAAAGAGATCAATTAACAGGCAAAGGTCCTTTAAGTGGGAATACACGTTCACATGCTATGAATCATTCAAAGCGTCGTTGAAATGTTAATTTACAAAAAGCAACTATTAAGACTGAAAACGGGAGCCAACGTGTATTAGTTTCAGCTAAAACACTAAAAACACTTAAAAAGCATAATTTATTAGCCTAGTGAACGAAAATGTTAAAAATAAAAAAATGTACAAACCATTGCTTGTACATTTTTTTATTATTAACTGGTTATTTTATTTAAAGTCCACTTAATTAAAACTCTAGTTAATAAACAAATTTATATGACTGACATTAAGTTTTAATAACCGTTTTTAATTAATAAATTTTTTAATGTTAATCATACATTATAAATATAACAAATTCAAATCTTAATTATATACAAATCATCAATTTTTATTGAAAAATGACGTTAGAAATAATTTATAAACACAAATATAAAATAAGAAGATTAAAATCTTAAGAAGAATATATATGTATATATATTAATATTCATATTTAGATTTTTTAAAAGATAATTGTTAAACATTAATCAAATTACTTAAAGTAATATGTTTAAAATAAAAAAAGAGGAATAATTCCTCTTTAATTATTATCTTCTTTTGGAAGAACACTAACTTTTGTTTTATCTCCCATAAATTTAGTGTATTTTACCACACCAGCAGTTTTTGCAAATAAAGTGTGGTCTTTACCTTGTCCAACATTTAATCCAGGGTAGATTTTATTGCCCCTTTGACGATAAATAATTTGACCTGCTTTAGTAGATTGCCCATCACCAAGTTTTGCGCCTAAATATTTAGGATTTGAGTCACGACCATTTTTAGATGAACCTACCCCTTTTTTAGAAGCAAAAAGTTGTAAGTCAGTTAATCAGTATAATTTATTCATTGTAATTATAAACCTCTTTTATATTGTTGTTTATATTCGTGTAATTCAATATAAGATTGATATTTAAAATAGATTGCTTTTAATTGAATTATAATTAATTCTAGATATTGACGATACAATTGATTGTTATTATCAATAATTATTAATATAAATCCTTGCTCAACCTTAATTGTTGTTTTTTGTTGATCAAATCAATTAAGTGCACCCATAATAATAGCACTCACTCCAGCGCATACTATATCTTTGCCATGCTCATCAAAATTTGCATGACCTTTTACTAATAAAGCGTTTGGATAATGATTTATTTTAATCATAAAAACTACTTAACACTAATTGAATCAATAACTAATTGTGTATATGGTTGTCGATGACCTTGACGTTTTAAATGGTGTTTTTTAGATTTAAATTTAATAATGTTGATCTTCTTTTGTTTTCCTTGTTTTATAACAGTAGCATTAACTACAGCATTTTTTACAAAAGGAGTACCAACACTACCTTCTACCATAATAACTTGATCAAATGAAATTTTACTACCAACTTCTAAATCTAGTTTTTCAACATAGATTTTTTCGCCTTGTTGAACTTTGTATTGTTTTCCGCCTGTTTGAAAAATAGCAAACATTACAAATTTCTCCTTAGAATTGATATCTTATCAACTTAGACTCACCAATAAGGTATAAATTAACATTTATTTTTAAACCTTTTTTGAGTGGTTGAAGCTGCATACGTTAATTTAAATCATACACAGCTTAAATATTATATCATAATTAAATAGGTTAACCTTGTTTATTTGGATGTTTTCTTTATATAATATAAATTAACTCATGTGTTATTTATTAAGTTGTAAATTTATTCAAAATTAGAGATGTAAAATTACTAAATTTTAAAAACATTGGTATTAATAATAACCATAAAAATAAAAAAGAGGAGTTTTAATTTATGAAACGCGGAAAATTAATTGTTTTTAGTGGACCTAGTGGTGTAGGAAAACATACAATTCTATCTAAAATCATAAATCGTAAAGATTTAAATTTAGCATATAGTATTTCAATGACAACCCGAAAAAAACGTGAAGGTGAAGTTAATGGTGTTGATTATTATTTTGTTAATGATGAAGAATTTAAAAAGGCCATACTAAATAATGAATTAATTGAGTGAGCTGAGTTTGTTGGAAATAAATATGGAACACCACGAACTATTGTTGAAAAATTAAGAGATGAAGGAAAAAATGTTATTTTAGAAATTGAGGTTGTAGGTGCACTTAAAGTTTTAGATTTATATAAAAATGATGATTTAATTTCTATTTTTTTATTACCACCAAGTATTGATGAATTAAAAAAACGCTTATTAAAAAGGAATACAGAAACATTAGAAACAATTAAAAAAAGAATTCAAAAAGCTACTCATGAAATAACAATAAAAGATTATTATCAATATAATATTATTAATGATAATCCAGATCATGCAGCAAACCAGTTGGCGGAGATTATTCTAAATGAAATTAAACAATCATAATCTAAAAGAAATTTATTCATTATCATTAAATGAACTTAAAGAAGAATTATTTAAATTAGGCTTAAAATCTTTTATTAGCAAACAAATTTATTCTTGGATTTATCAGAAACGAATTTTTAATTTTGATAAATTTTCTAATATTGCTAAAAATAATGTAATAATTCTAAAAGAAAATTTTAGTAATAATCTATTAAAAATTAACAGTTATCAATCAAATTCTGATGGTTCAATTAAGTTTGAATTTTTAACAGATAAAAATCTGATTATTAATTGCATGATTATTAAATTTAAAAACGGATTTTTAATAAAAATAAATCCGTTTGGAATTAATTTTAAAAAAGAAATTATTAATTTATCAACTAATGAATTAGTATTACAAATTTTATTAATACAACAATTTCTAGATCAACGTAAATTAGGCAACATTACTAATGTTATTATAAAAGGATTACAGGATAGCTTATTAAATATGGACGTTGTATCAAACTTCATTAATATTATTAATAATGAAAATGGTTTAAATATTGGAAAAAGGAAAATTGCAGTTTGAACAAGTGGTATAAATGTTGATTTGATTAAATGAGGACAATTACAAAATCAAATAGAATTGATTATTAGTATGAATGCTTCAAACTCATTAATTTATCAAAAAATAATGTTGAAGAAACTAAATCAAAATTGATCTTTTTTAAAATTAATTGAGCAAATTAAGGCATATACAAAGATTACAAATAATCGCGTTGTATTAGAATATTTATTAATTGATAAAATTAATGATGATTTAAATTATGCTAATGAATTAGTAGAATTATTAAAAAACATCTTATGTTATGTGCTTTTAATTCCTTATAATTTAAATAATTATCGAACTAGTGCTAATTTAGATGATTTTTTTAATATTTTATCTGTTAATAAAATACGAATTAGTAAGCGTATACGAAAAAGTAACGATTTAGATATTAGTTTTAAACAATTGAAAATTAAGGAGTAAAAAATGAATTTTGGGTTTATTAGTGATATTGGCTCACAACGCAAACATAATGATGATTGTGCATTGGTAATTCAAAATCAATATCAACAAACTTTATTAATTGTTTGTGATGGATTAGGCGGGTATAAAGGTGGTGCTGCGGCTAGCCACATTACATTAGAAACAATTAAAGATAATTTTTTAACTACAGATTTTAGTGAGTATGATAAACAACAGATTCAAAAATGGTATATTAAAGTTATTAAACTAGCACAGGTTGAAATTGATCGCACTGTTTTACTAGATAAAGACGTTTATAATATGGGAACTACAGTTGTTGCTTCTATTGTTGTTAATGATTTTTCATACACTCTAAATATTGGTGATTCGCGTGCTTATTTATTATCCAATGAACAATTCTTACAAATAAGTCGCGATCATAATTTATTACAAGTTTTACAAGAACGTAAAGCCAACCCTGAGGTTTATAAAAAACATGAGAAGAATTTATTTTCATTAACACAATTTGTTGGTCGCACAAGTAATATTTTTTTGAGTTATGACTTATTCGTAAATAAATTAAACCCAAATGAAATTATTGTTTTAACATCAGATGGATTCCATAACTATTTTGAATTAAATACATTATATGAAAAATTAATAAAAAGCACCCAAGAGACAAAAAATGAACTTTTACAACAATTAATAAGACAAGCTATTATCAACGGTAGCAATGATAATTTATCATTAGCTTTCTTAATTTTTTAAATTTTATGCGTGAAGAAATAAAAACAAACTCAATTCTTAATTATAAATATAAAGTTGTTAAACACCTAGCAGATGGTGGTTTTAGTAAAGTTTATTTATGTTGTTTTTTATCAGATGAAACTAAGTTTATTGTTATTAAAGTTTTAGATATTAATGATGAGAAACAACAAATGGTTGTTTATGATGAATTAAGAATTTCAAATTTAATTAAAAATTCTGATTCTGATAAAAAAAACTATATTATGGAATATTATGAATATTTTGAAACCGGTTCGTTAGAAAATAATGATAAAAGGATTTATATTGTTTTTGAATACATTGAAGGTTTGACATTGCGTGAATATCTTGATGAATTTAAAACAATTACTTATGTTAAGGCTGTTGAAATTATTCGTCAGGTTGCCTTAGGTGTAAGTTTTTTTCATAGTTGTAATCCACAAATTATTCATCGTGATTTAAAGCCAGAAAATTGCATGATTAATAAAACCTTAACAAAAATTAAAATTATTGATTATGGTGCAGCATCGGTTTTTTACAATCGTGAAGATTTAACAAAAGATCAAGAAATTAAATGTACAATTATTTACGCTTCACCAAAATTATTAAGTCTTGGGCAAAAAGTTAAAGAACAAGCTAATAAGGGTTTAAATAAAAGAGCTTTAAGTTTAATTAATGACGCATTAGGTGTTAGTTATGATATTCATTCTTTAGGAGTTATGTTATATGAATTAATTACTGGAACTAACCCTTTTAGTGAACATACAATTACAGATGATCGTGATTATTTAGAAAAATGAATAACATATGATGTTGAACCACTATCGTTAATTAATAAAACAATACCAAAAGGTATTGATAATATTTTAATGCGTTGTTTTGCTTGAAAAAAAGAAGATAATAAGTTGTTATACAAAGATATCTACACTTTAATTGATGATTTAAGTAATGTTATGGATCCAGAGTCAAATCTTAATCAAGAATACATTAAACCATTAAATAAGTTACGAATTTATCGTAAAAATCTAGCAGGCTTATATGAAATTAAAGAAAAAGATCGTAAATGATATTTACAAAAATGATTTATTATTCTTATTAGTTGTTTATCGGTTATTCTAATCATTTTATTAATTATTATTTTATTTTTTAAAAAAAGTGGGGCAATCTAATTGTGCGGGCAAAGATTATTTCGGTTGTTGCTAATAATTTTTATGTACATCTTTATGATTTAAAAATTCAGATAAAAGCAATTCCCAAAGGGATTTTTAAACATAACAGTCATGAATTAAAACCAATGGTTGGGGATGATGTTGAAGTTGAATTAATTGGGAATACTTATTTAATCACAAAAATTTATGAACGTTATAATCAATTAATTCGTCCCAAAGTTGCAAATATTGATATTGTTTTAATCGTAGTCTCTATTGTACAACCAGATTTAAATACATTGAGTTTAAACAAATATTTAGCTTTTTATGAAGCTCGCAATATTAAAAATGTTTTAATAGGTTTGAGTAAATATGATTTAGCCAATAATCATTTAAAACAAAAAATTAATAAGTTAATTTTAGATTATAAAAAAAATAACTATGCGGTTTTTATATTAACAAAAGAAAATGATATTTTATTATTAAAAGAAAAGATTAAAAGCAAGACACTTTGTTTGGCAGGAAATTCAGGCGTAGGTAAATCAACATTAATTAATAAATTAGATCCAAGCATTCAACAACGTACACAGGAAATATCACATTTTCTGAATCGAGGAAAACACACAACAACAAGTACCAAACTAATTAGTTTTGCTAATGGCTTTTTAGTTGATACACCGGGTTTTGGAAATTTAGAGGTTAATTTAACCAAGAATGAAATGGCTAATGCCTTTAATGATTTTGCAAATTATGCACGTTTTTGTAAATTTTCTAATTGTTTACATATTGAAGAACCAGAATGTGCTATTAAAAAAGCTGTTGATGAAAAATTAATTGTTAATTGACGATATGATGACTATTTAAAAATAATAAAAGAATTACCAAATGATGTGTTGAAAATAAAAACACGTGCTCAAAATAGAAAATGTAAATAAATAATTCTAATTCTTTAGTTGCAAAAATACTTTTTATAATTCGCTTGTGCTTGCTTTTTATTTTTTTAGTACTATAATAGTAATATGTACTTAAAAATTATATTTTGTTTTAAAGTATAAAAAAAATAAATAAGAGGTGAGAATTGTGTCAACAATTATAACTACATCTTGAGGAGTAGATCCAAATATTTACTTTTCAAAGCATGAGTTAACATGAGTGCCAGCAACAGCTGATGCTAATACATTAGCATTAACTAATTTTAATCCGACTAGCACTTTATTAGTTGCATTAGCAATTGCCTTTGTCTTATTGATGACTCCAGGTTTAGCATTATTTTATGGTGGGCTAACACGTCGAAAATCTACATTAACGATTATTAATCAATGTGTAGCTTCACTTGGAATCACAACACTGATTTGAATTTTTGGAGGGTTCTCACTTGCTTTTGGACCTTCTGTTGGTAAAGGAATAATTGGTGATATTTCAACTTATTTTGCATTTAGAAATCTATTATTTGCTGATGGTTGAAGTGGAGATGTAGGAATTGTTTTTGCAAACTTTACTAATGGTGTACCATTAATTTTATTCTTTGCTTATCAATTAGCTTTTGCAATTATTACACCACCATTAATGGTTGGTGCATTTGCAGATCGAATGAAATTTAAAAATTATCTAGTATTCTTAGTACTATGACAATATTTAATTTACATTCCTTTTGCACATTGAATTTGAGGTCAAGGATTCTTAGCTGCTGCTGGAGTAATTGACTTTGCTGGTGGTATTGTAATTCATACAACTGCTGGTTTTGGAGCATTAGCTGCTTCATTAGTATTAGGTAAACGTGTTTTACTTAAGAATGATAAAAGTCGTCCACACAATATTCCAATGACTATTATTGGAGCAACATTGTTATTCTTTGGTTGATTTGGATTTAATGTTGGTGGATCAGGATTTGTAGCTGGTGGGAATGCTGCTACTTGATCAGCTGCTACAAGTGCTTGGATTAATACAATTATTGCTTTAGCTATTGGAATGGTTGGTTGAGCAGTTCTTGAAACAATCTTTAATAAAAATCATAAAGCCACAGGTGTTGGTTTAGTAACAGGTGCAATTGCTGGATTAGCAACAATTACTCCTGCAGCAGGTTATGTTCCAATTTGAGCATCTGTTCCTATTGGTGTAGCTGCTGTGCTAGTTTGTTATACTAGTGCTAAAACTTTACATCATTTCCACAAAATTGATGATACTCTAGAAGTATGAGGCGTACATGGAATGGGTGGTGTTACTGGTTCATTATTAATTGGTGCGTTTGCATCTAAATCTGTAAACCCATCAATTATGTATGAAGCAATTGGTCCAACAACAACTGGTGTTTTATTTGGTGTCCAATTAGGAGCGGTTTTATTAGCTGCGGTTTATGGATTTGTATTTACTATTTTATTAGTATATATTACTCGTCCAAGATTATCAGCACGCCAACAATTAGGTCATATAGACTATATTAATCATGGCGAAGATGCATATTCATTCGATATTGAAATTCCATCTGAAAAAGAAATGGAGCAATATGAATTAGCAGATAGTACATGACACTCAAGCACAGGTGTTAAGTATTCATTGCTTAATAATAAAAACCAGCAAAATCATAAAGTAGCAAACGACCATTAATTTTTAAAAAAAGACTAAAGCAAAATACTTTAGTTTTTTTAATTAATAAATAATAAAAAAATAAAGAATTTATTAATTAACGTAATATAATACTATAGATATATTTTGTATTTATAAATTTATGTACAAAATTTATTTATCGAAAACGAAAAAGAAAACACGAGGTGAAAATTGTGTCAACTTGAGGCCTAAATAAAGATATTTATTTCTCAACTCATGAATTAACATGAGTGCCCGCAGATGCAAGTCAATCAACACTTAATGCAATAGGTTTTAATCCTACTAATACACTACTAGTAGCTTTAGCAATTGCTTTTGTTTTACTAATGACACCAGGGTTAGCATTATTTTATGGTGGTTTAGTAAGACGTAAATCAGCTTTAACAATTATTAATCAATGTGTAGCTTCACTTGGAATTACAACATTGATTTGAATTTTTGGAGGTTTCTCACTTGCTTTTGGACCTTCTGTTGGTAAAGGAATAATTGGTGATATTTCAACTTATTTTGCATTTAGAAATCTACTATTTGCTGATGGTTGAAGTGGAGGAGCAGTTTTAGTTTTTGCTAATTTTACTAACGGCGTACCATTAATTTTATTCTTTGCTTATCAATTAGCTTTTGCAATTATTACACCACCATTAATGGTTGGTGCATTTGCAGACCGAATGAAATTTAAAAATTATCTAGTATTCTTAGTACTATGACAATATTTAATTTATATTCCTTTTGCACATTGAATTTGAGGTCAAGGATTCTTAGCTGCTGCTGGAGTAATTGACTTTGCTGGTGGTATTGTAATTCATACAACTGCTGGTTTTGGAGCTTTGGCTGCTTCATTTATGTTAGGAAAACGTACAACATTAATTACAGATAAAAAGCGAGCTAATAATTTACCAATGGTTGTATTAGGTGCAGCATTACTATTTTTTGGTTGATTTGGATTTAATGTTGGTGGTGCAGGTTTTGTTAAAGAAAATACAAATCACATTTCACAAGCTATATCATCATGATTAAATACAATTATTGCCTTAGCAATAGGTATGATAGGTTGAGTTACACTTGAAACTATTGTTAACAAAAACCACAAACCAACAACAGTAGGTTTAGTGACTGGTGCAGTCGCTGGATTAGCAACAATTACTCCCGCAGCAGGTTTTGTTCCAGTTTGAGCCTCTGTTCCTATTGCCATTATGGGTGTTTTAGTATGTTATTCAGTTTCTAGATTATTAGATTACTTCCGTTTTGATGATTCTTTAGAAGTTCTACCAGTTCATGGAATGGGTGGTGTTATAGGTTCTTTATTAATTGGAGCATTTGCTACTAACACGGTTGCACATGGAATTAAATATGACGCCGTCGGATTAGATAATAATGGAAATCCAATTTATGGTATTTTATTTGGACTACAATTAGGTGCTGTATTATTAGCAGCAACATGGGCATTTGCCTTTACATGTTTAATAGTTTATATTACTAAACCTCGTTTATCAGTGCGTCAACAATTAGGCCATATAGACTACATCAATCATGGTGAAGATGCATACAAGTTTGATTTTATTATTCCTTCAGAAAGTGAAATGTTTGAATATGAAAGTTTTGATCAAACTTGAGAATGTAAAGCTAGTGGTTGTAAAAAAAGCAACTTAGCTGCCAAGAAAAACCCTGTTTTTATAGAGAATGAGAATGATTGTACATGACAATCTAAATCTCGTGAGTGTACAAAATAGAAAATATCAGTATTTCATAATTAAGAAGTTAATTAAAACTAACTTCTTTTTTATTTATAATTTATAATTGATGAAATAGTAATATTTATCTTAAATGGGGGATCAATGAATGCCACGAATAAATACGCAAGTTTTTAATGAAAATAATGATAAAAATAGAATTAAAAGTAATAACAATAAAATTTGTGATGATATTGAATACACTCGCTTATTAGAAACGAAAGAATATAATGCTGGTTTTTCTCCTAATAATGATTCACAAGATATCTTAATTAAAAAACAAGACAATATTTTAAAGTTAAAACCAACTCTAAATTTTGATGATAGTGATCAATTAATTTCAATTGTTCCTACTAAAAATTATGAAAATTTAAGATTAAAAGAAATTGAAATCGATAAAAAAAACACTGTTGATTATCAATTAAAAAAAGAAATAATAGATCAGGACTTAACAGATGATAATCATTTATCTATAAGTCAAAAATCCAATGGTTTTGCTTTAAAAACTAAACAATTTATTCCTCAAAATATTTCAGAATTTATTTATCATAATGAAGACGATCCAAAAACATACGGAAGTGAAAATAATTTAACTTTTAGTCAACTAGAAGAAAAAGTTAATCAAAAAAATACACCTGATTTTTATTTAAAAGAAATTCATGCAGTTCGTAACAAAATTATCATTTGAACTATTTTATTAGCAATTGGCGTAGGATTAATATTTTATTTGATTTATGAAGTTATTATTAATCATTTTAATCCATGGATTTTTTTACCAATTAGTATTTATTGTGTCTTGATGATTTTCTTTTTAGCTTTATCATGTAGTAATTTTATAAATTTTAAAAAAGAAATGGATTATCAAAAAAACCATTTTGATTGTACAAAAGCAACAAATTTTATTGTAGGTATATATCGTAAATTAATAGTAGTACATATTAATGTAAACTGAATCACAGCTTATATTTATCTTACTAGTCTTTTTATGATATTTGGTGTTTTTGTTGTCAGTTATTTTATGAATTTATATTATAATGCAGCTATAGGAAGTCGTTTTGGTGATTTGATTATAAAAATTCCTGTTGTTATTAATAATCATCTTTCACAAAAAATTAGTTTTAACAAAAATCCATTATGGGCAATGATTGGATTAGGTGCTATTTTAGGATTAACATTTATTATTCATTTATGGTTGGTTTTTAATGCTCATCATCGTATTAATCGTATTCATAGTTATTATGTTAATGAAATCATTACTAGTGAAGAAATAATTTCATTAAAAAAACAAGCAAATCGTCGTGGTCTAGTCATATTTTTAGTGTTAACTGTTATTTTAGGTTTAATTTTTTATTTGATGTATACTATTTTAAAAAGAAAATACACTATCAAAAAATAAAAGAAGGTTTATATATGGCAAAAATTATTGAAGAAATGATTAATAAAAAAGGGTTGTTAATTGATGATTATGAACTTTATGGCAATGAGATTGCTAAAATTAAATCAATTAAAAAACTAAATAAAAAGGATTCAAAATTAATTGTTATTACCTCAATGAATCCTAATCCAGCTGGTGAAGGTAAAACAACAACTGCTATTGGTTTAGTTGATGCTTTAAATAAACATGGATACAAAGCAATTGGAGCCTTACGAGAGCCATCAATGGGACCAGTTTTTGGTATGAAAGGTACGGGTTCTGGAGGAGGATTATCTTTTTTAAAACCCTTTGATAAAATTAATTTACATTTTAGTGGTGATTTCCATGCGATTACTGCAGCTAATAATTTAATCGTTGCAGTAATAGAAAATGAAATACATAATAGGTCTTCAATGCAAATCGATTCTCAAAAAATTTTAATTAAAAGATGTCTAGATGTTAATGATCGTTCTTTACGTAATATTGAATATGATATTAACCATCAACAAACAAAAAGTGGTTTCAACATTACAGCAGCGAGTGATTTAATGGCATTATTTTGTTTAGCACATGATCATAAAGATTTTGAAGATAAACTTGCGCAAACAATTGTTGCATATAATATAGTAAACCAACCTATAAGAATTTGTGATTTAGAATTAACAAAAGCTATTATGGCAATTTTAGAAGATGCTTTATATGCTAATTTAGTACGAACTAATGAGGATAACCCTGTTTTTGTTCATGGTGGCCCATTTGCTAATATTGCTCATGGTTGTAATAGTATTATTGCAACCAAAAATGCTTTAGCTTTAGGTGATTATGTTGTTACTGAATGTGGTTTTGGTAGCGATTTAGGACTTGAAAAATTTATGAATATCAAGATGGCTAGTTTGAATTTAAAACCAGATTTAATTGGTTTAGTTATTAGTCTAAAATCAATCGCATATCATGCTCAAACAAACGAAAAAGACTACGTTAAACAAGGCTTTGCTAATGTTTTATGTCATATTAATCACATTAAAAAATATAATGTTTCTTTTATTGTTTATATTAATGTCAATACTAATACTGATAGCGAAGAAGACTTATTAACTTTAGAAAAACTATTAGATGAACATCAAATTGAACATGCACGTTCTTATGCTTATAGCTATGGTTCTAAAAAAAGTGAAGAAATAACTAAAAAAACAATTACTTTAACTAATCAAATTAATGATCATGAATTAAAATTAATTTATGATATTAAAGATCATTTATCATATAAATTAAAAAAAATATGTGAAAATGTTTATGGTGCTGATGGATATGAATTAAGCTATGAAGCTAAAGAACAATTGAATCGTTATGAACATTTAGATTTTTATTTATGCATTGCTAAAACACCATATTCAATTTCTGATGATGCAAAATTATTAAATAATCCTAAGAACTTTAAAATTCATATTGAACGCTTTGAAATTAATTATGCTGCAAAATTAATCATTGCTATTACAACAACTATTTATCGTATGCCTGGCTTAAATAAAGAACCAGCGGCCAAAAATTTCGTTATGAAATAACTATTAAAAATCTTTATTATTAAAAAAATAAAAAATAAACAAGTTGATCGCTTCAAAGATGCTTGTTTATTTTTTTCATACTTTAGAAGATAAAAAAATAAGAAATCATAAAAAGGAAAAGTAGATAATATGAAAGATTTTATTAGATATACTAAAAAAAGAAATTTAAGTCTTAACACAATTCGTACTTATGAATCAGTATTAAAACATTATGAACCTGTTTTAGATTCGTGAATTAAAATACGAAATAAAATTATTAATAGTAACTTTAAACCACGAACAATTCATTTACACAAAAACGTTTTATTAAGTTTTTTTGAATTTAAAAAACTAAAAAGATATTTACAAAATTTAAAATTATTAAAATTACCACAAATTGAAATGAAATATTTTGATGTAATTAGTAAAAATAATTTATATAAAAAAACTGATATCTTAGATGATGATTCATTAGAAATTAAAAAATATAAAACCATTATTAGATTCTTGTTTGAAACAGGAATTAGAGCTCATGAATTATTTTTTCTAGAATCTATTAATAATCGTTTGTATGTGTTAGGTAAAGGAAATAAAAAACGACAAATTTTCTTTGTAAAACAAACTTTTGAACAATTACAAAAGTTTTATGAGAATCTAAAAGGATTTGAAACAACTAAAACATTACGTTTATATATTAAAAAAATTATTGGCAAGAATTTTACTCCCCATTCATTACGTCGTAGCTTTGCTACTTTTATGTTAATTAAAGGGGCAAATCCCAAAACTGTAATGTTACAAATGGGACATGCAAACATTCAAACAACGTTTAGTTATTTAAATTTAAATGAACAAACAAATCGTCGAATTTATAACAAAATTATGTACCAAAATGATGCAGAATAAATTTTTGTAAAAATAAAAAATAAAAATGTTAAAAATCGACATTTTTATGCTAAAAAGCATAGGAAATACTAGCATTTATTATATAATCTAAATTAGAAATTAGGTTTTATATATAGTAATGAATTTATGAGCAAATTTTTGATTGGTCGTAAGCGATTTATCAAAAATAAAATTAGTTTCAAATATTTTTAGAATAAGAGGTGATAAAAAATTGATAAATAATAAAATAAAAGAAGCAAAAATCAAAAAGAAACTAGAAAAAAACAAAAAAGTTTCTTTAATGGGTTTTGTTTTACTAACAGCTTCAGCAATTATTGCTTTTTATACATTCCCATCACTTTCAACAGCTGGTTGAATTGCAATTATCTTTGCTTTTATTACAGCTATGTGTTGATTCATTCCAATTGGGATTGCAGCCGCTGAAATGGCAACAATTAAAGGTTGGGCCGAGGGGGGAATATTTACCTGGGTACGAAATTTATTAGGTCCACGAAATGGGTTTATTGTAACCTGATTACAATTCCAAGTCACATTTGGATTTGTGGCTATGATCTTATTCGTCCTTTCATCCTTTTCATTTGCTTTCGCAGGTGCTGATGGATATAATTATTTTAATACACTTAAGTTGGGAACAACTTTAAATCAAGCTACATCAGTCAACTTTTCAAAAAACTTTAGCAATGGGGCATTATATGGAATTGGTATTGTTGTTGTCGCAGGTCTAATCTTTATGAGTTTATTAGGCCAAAAACGAACTCACCAATTTGGCCAAGTTGCTTTAATCATTGGTATTTTACTACCATTCTTTATTATTTTAGGATTCCAAATTTATACAGTGGCAACAATGCCAGATCCATTATACTTTATTGGAAAACACTTCCAAAAAGAACCACATTTAGACCCAAGTGTAACTGGTACTTCTTATGAATTAAATAAAACTTTCATGTCATCAGTTGTTATGGCATCTTTCATGGCATTTAGTTTTAGCTTACATGGTGTAGAAGTTTCAGCTATTGCTGCTAATCGAATGGATAATCCTTCTAAGAAATATCCACAAGCCATGATGATTGTTGTAATTTCTGCTTTAGTTTGCATTATTATTGGATCAATTATGATTTCAATGACTGTACCAACTTCAACATTATCATTCAATGGTGGATTAGTACAGACAATTATGTTCAACTTATCTGTTGGTGGAATTGATGTTAAACTACCAGATGGCACAGTTGCTCATTATAAAACACTAGCACATGCATACTCAGTAATTGAAACTCAATATGGCACAGCTGCCGCTAACCAAGTTATGTATCAATTTGTGTATGGTCATCCAATTAATACAGGTAGTGACTTAGCACGTTCTGATTTATTAACACTAACATCAGAAGTTAACGATCCTTTAAAAAACCCATCTTTATTTATTTCTGTTATGAATATAGAAACAAATAAAATGGCTATGCAAGGATTACAAGCTATTACTTTCTTCATTGGAATAGGTGTATTTGTTGAAGTTTCTGTTTGAATGTCAAACCTTTCAACTGGATTAAATTATGCAATGCGTAAAGCCCACTTCCCAGCATGGGTTACATATCGTTTAAAGAATGGGTCAGCGTTAACAGTAGCAATTTTATGAATTGTATTACTAATGTGCATTTTTGCAATTTACATGTTTGGTTATAATTCATTAATGCAATACAAAGGTCAAACAATTAGTGATATTTTAGCTAATGAAATTCGTATTAACCAAGATCAAAAAGAACCAATGGCTTTATTGTCTAATCAAAATTTAGATGAAATTAAACTAATGGCTCGTTCAATTTACGAACATAGTCATGCTCCTGGAGCACAAGCTAATGTTTTACATTTTGATTTTAATAAACATGCTAACAGTGAAGTAAAAGCAATTTATGATAAAATCAAAGATTATATTGGCATTAATGCCGTAGCATCGGGAACACCAGCATCAATTACTAATATTTCATTCATTTCAAACGTTGTTTCACAAATCTCAATGTATTATATTGGATATAACATTTTCTTAATTGGATATCTAAGATATGTCTTTAAAGCTAATTGATTACGTCGTGAATTTAGAGTTAAATGATCATGATTACAAATTAGTTTAGCTTTATTAGCAATGGGCGTTAATACATTTGCAGTTGTTGCAACTTACTTACCAGCTGCTCCAGAACTATATCCTGGACAAGAAGCTTATTATGTATTTATTTCAATTGCATTACCAATGTTTATATGCACATTATTACTTGGATTATTTATTTATCTAATTAATTATGCTTGAAATAAAAAACGAGGTATTAATTTATCAGAACATTCAAACTTAACAAACGTTGATCAAAATGTTTTAGATGTAATTAATAATTGACATTTATACAAAGATCAAAAAGAATTTGCTAAATTTGAACGTCATGTGCAATTATTACAACAAAAACAAGTAATTGTAAGAACAACAAAAGATAAGGCACAAAGAGAATTAGTTGCGAATGAAATGCAATATTTAGCAGCTGAAATCGCAACAAGATTTGCACCAGAAGAAGAACATTAAAAAACAAAAATGAGTCTTAATACTTTTTTAAAAAGTGTTTTGACTTATTTTTTTATTTTACACTTGACATATTATATATATATATATAATCTAAATGTATACTCTTATATAAAATAAAGATAACAAATCAAATTATTAAAATTCTTATTTATTAAATGTATAAAAGAAGGTTAAAATATGAAGAAGAATCATAAAAAATGATTATTAATGACAACGCCAATTTTATTAATTGGCGGATTATCAACATTGGCATTAACAAGTTGTAAAGACGGAAAAAAATCTAATCCAAAACCAGATAAACCATCAACTATAGAAGAATATAAATTAAGTTTATCAAGTGTTGAAACTGGTTTTAATGAAGCTAAATTAATTTTTGATAATAATGATAGTTTTAAAACATTAGATAATTTTAAAGTTATTTTAAAAAATACTCAAAACACTAATGAAGTTATTGAAGCAAGTAATTTATCTGCAACAATTGAAAATCAAAAATTAGTTTTAAAACTAAATAATTTACAAGACGGTCAAACATATAAAATTAACAAAATTTTATATCAAACAAATAAAGAAGTTATTTTAAGTAATAATCAAGTATTTACTACAAAAACTAAACCTAAAATTGAATATGAAGTACAAGAAAAAATTTTGAAACTTGATGATAAAACATATTATGTTGTTCTAAAAGTTAAAGACCCTCAAAATTTAAATGAACCAACAAAAGAAATTTTAAAATCTGTAACTTTTACTTCAAAAATCTCAAATGTTGCAAATCTTAAAAATGCAATATTAGAAAATCAAAGACCTTACGTTAAAGAAGATTATTCAGAAATTCAAATTAAATTACCAAGAGTACCTGTAATTAGTGAAAAAATAGAAATCACTGCAAACAATTCCTTATTTAAATCATTCACAATTACTGTTGGTGAAAAACATATTAAAAATGTGGAAAAAATCATCGATATCACTAAAAAAGTTTTTGGTAATCAAGAATTAAAATCTATTAGTATTACAAAACAAAATAGTAGCAGTGCCAATATAACATTAAGTTTAAAATTAAATGGTAATTCAATTATTGTTGGTGCTAACAAATTAATTAATAAAAAAGACAAAGCAATAAAATTTAGCTTCTTTGTTAAAAATAAAGCAACAAATACAGTTAAAGAATTTAATTTCAATGAAAAATCAAAACCTATTGGAAGCTATCAATTCACATTAAATGGATTAGATGCTAATAGTGATTTTGTAATTGAAGATATTAGATATGATGATAAATCAATCAAAATTACAGAAGAGCTTTTAGCTAGCTTAAGTTTTTCAACAAAATAAATTAAATACATAATTTATTGTATAAAACACAAAAAATATAGTTTAGGAAATGAAATATGAAAAAATGACAAAAAATTTTAACAATTGCACTACCAACTAGTTTATTAGTTGCTATTCCAATTGTTGTTGCTTCATGTTCTACTAAGAGTGAAGCACAAAAAGAATTTGAAAAAACTTATAAAGAATATTTAAGTTGATTAGACAAATTAGCTTCAAAAATGCCAGCACTTAAAGAAGCTTTTAGTACTTTGAAAGAAGAAATTAATAAACAAATCAATAAGAGTGAAAAATTATCTGATGAAGCGTATAAAGCATTAACAGCTTCATTAAAAGTTTCAATTGATGGAATGAAAAAAACATTAGGACAAAATTAAGGATAAATTCGTGAATCAGAACAAAATTGTTAATAAAAAAGATTTAGATATTGCATTAGCAAATTTAATTTTAAGTATAGGAGTAGTTGTTTTATAATAATTGGCGCTATTTTAGTGCATCTTGGCATTTATTATATGGTTTACTCATATTATGGATCACCATATTTAACGCAAGATATAGCTACAACAAATGTTGTAAATGCTTGAGCTATGTATAATGCTGGAGTGGCTTTTTTAGTAATTTTTGCTTTAATTGAAATTGCTAGTTTTGCTTTATTTGTAACAACAACAGTCAATGTTGGTTTAAATTTAAAAAGACAAGAAAACTTTGTCTTGCCATTTGTTTTTGGCATTTTAGGGTGCTTAGGAATAATACCTATAATCGGATCAATTATTATTTATGTACAATTAAATAAATTTTATAAGCAACAAAACGAATCTAGTTTAATATAAAAGAAAAAAATGACTTTATATTGAAGTCATTTTTTATTACCTTTTTAATGTTCCAATTATTATACTAAAAAGGTAATAATTATTACTTTGTTTCTTTTTTACGTCTTTTTTGTATATATAAATATATAATTAACCTTTTAATTTGTGTAAATGAGAATTTGTTTGGAGAAAATCATGAGAAAAATTAAATTAAATAAAAAAATTATTTTAGCATCAGTTATTGGAATAATGGGTGTAGCTTTGGTTCCTATAGCATTGGTTGCATGTAGTCAAAATAAAACAAAATCTGATTCTAGAAACTTAACTTTAAACAATTTTTATGGTCGCCCATCAAGTGATGGGGATGATGCATATCAAGCAATCTATGCGTCTAAAATTCAAGATGGTGCACGTATGTTAGGATTAATTTCATTTAGACATAAAAATCCAATTTCAAAATACTTTAATTCACCAAAAGATAATCAACAAGTTTCAGCAGTATTAATTGATGAAATTTATGATTTACAAACAGGTAAAGATCGAATTGCATCAATCACATATCGTGCAGATCAAGCTGCTTTTTTAGCTGGAATTGCTGCAGCTTATTATTTAAATAGTAATCAAAATGTTTTTGGTAAAGATAATAAATTAACATGGGGCGGTTTTGTTGGTATTCATTTACCATCAACTACAAGATTTATTCAAGGTTTTAAATTTGGTATTCAATGAGCAAATGAAAAACTAAAAAACAAAAAAGTTAAACAAACAGAAAATAATGAAGAAAAAGAATGAATTAATGTAGAACAAGTCTTTGCTACGAATTATCAATCAGGAGATTTTTCGCCAACATCAGATAAAGCAAAAGCAATTGTTAATCAATTAGTTTCTAATAATGTTGATTTAATTTTACCAGTGGCTGGACCTCAAATCGATTATGCAACAACAGCTGCTGCTGAATCAAGTAAACCAATCGTTGTTGTTGGTGTTGATACAGAACAAGAATTAGATGATAATACAAATAAAGCACGTATTAGTGAAAATAATAAATCATTAGCTAACGGTAAAACAATTATTTTTTCTATTGTTAAACGTTTAGATTTAGCTTTTAAAGGTGCTTTATTAAAAGCATCAGAAGGGGCACAATTAACTAATGATATTAACAAAGACGCTTATAAATTAGGAACACACACTGAAGCAAGTTTTAATAAAAATACTTATGTTGATAATACAGCGTTAGTTGAATTATCAAAAGCTGGACATCAGTATTTAATTGATGCAATTAAATTAAGTGGTTTAAAAGAAGTAAATGATTATAAAACAATTGTTGAAATAATCCAAGAAGATCCTTTATTTAAACTTTTATCACAAATTGGAACTAAAAAATTAGATGAAGTTGCTACTAAATCACAACAAGGTGATTGAGTATTAAAAAGCGAATATCAAGATTTACCATTTATACAATTACAAAAAATGTTAGGTGGATTAGTTTATGTTGATCAAAAAAATGAACTATATCCATATGAATTATCTAATTCATTTTATTTAGAAAAAGATCCAAATAAACGTCAAGCTTCAAGAGCTTTTTATAATTATTGAAATGCTAAAGATGCTAATCAAATAAATTTAGTTAAGATATTTTTAGGACAATCAGTTGATGTACTAAAAGATAAATCATTTTCTGAGTCAATTTATAAGGGATTAGAAGAATTTTATAAATCTAAAAACATTATTATTCCTAAATTATATTAGTTAACTTATTAAGTTGCTTTTTACCCATAAATAATATATAATTCTATATTGTATAAAGAAAGTAGTTTAAAACTCACCTGATTTCTGTTTATTTGGGAATAGGTTAATGCTTAATATTTCGATTATTACATCATTTATTCATGTTATAGATATAAATGATTTTCTTTATACATTTAATTTTTAATTAGGAGTGTTTAATGAATACACCAAACAATCAACGCCATATGTCATCAAATAATGATGCACGTAAAAATCAACCTTTAATTAATGATCAAATTCGTTTTCGAACAATGGTTGTAATTGATGACCATGGTAATAATTTAGGTGAAATGAATCGTATTGATGCCCTTAATTTAGCCGCTTCAAAGAATTTAGATTTAGTTGTTATTGCTAAAAAAGGAAATATTCCTGTAACAAAAATTTTAGATTATGGTAAGTACAAATACGAACAAAAACGTCGTCAAAAAGAATCAAGAAAAAATCAAACAATTATTAAGGTAAAAGAAATCAAAATTAAACCAATGATTGGTGAACATGATTTAAAAGTAAGAGCAGAAAATGCTAAACGTTGATTAGAAGATAAAGACAACGTGAAATTTGTGATTGAAGCACGTGGACGTATGTGTACAAAAGATGAATTTATTGTACAAGCTTATGAAAAATTCATTGACTTAATTAAAGATTATGGTACAGTTGTTCAAGCTAATAAAAAAGTTAGCAATTATCGATATGAAACAATTATTGAACCAATTAAAAAATAAGGAAGGTAACTATAATGGCTAAAATTAGACAAAAAACAAAAAGAGCTGTTGCAAAACGTTTTAGTATTACAAAAAATGGTAAATTAAAACGTAAACACGCTTATAGATCACATTTAGCTCTAGGTCGTTCAACAAAAGCTAAACGTCATTTAAGAAAAGACGCAATTATGAGCACATCTGACACAAAAAGATATACTCAATGCTTATAGTTAGAATATAAATAATTTAATAGTTATAAATAGTATTTAAGGAGTAAATTAGCAATGAGAGTAAAAGGTGGAAGTGTTACACGTCAACGTCGTAAAAGATGATTAGAAAAAGCTGAAGGTTCATGAGGAACACGTAATACCTCATATAGAATTGCTCGTCAAACAGTAATTCGTGCTGCAGAGTATGCATATCGTGATCGTCGTAATAAAAAGCGTGATTTTAGAAAATTATGAATTTCAAGAATTAATGCTGCAGTTCGTGAATTAGGATATACATATTCACAATTTATGAATGCATTAGTAAAAGCTAATGTTGTTACTAAAGATGGTCAAGGTTTAAATAGAAAAATGCTATCTGAATTAGCAATTAACAACCCTGAAGCATTTAATCAATTAGTTGATAAAGTAATGAAATAATATTAGATAAAAATATACTTGCCAATTGCTGGTTATAGTATATTTTTTATTTTTATCTTGTTTTTTACTTTTGTTTCGTGTATAATTTTAAAGGTTTTGTTATTGTTGAGATTGCTGATACGCCAAGAAACGTTGTCAATAAAACGTATCAGGTAACTCATTAATACACAATTCTTATGATTCGCATAATATATCGATGCAAAAGGAGACAACATTTTATGAATCAAGAATTAAGAATTAGATTAGAATCTTATGACCACAGATTATTAGATGATACAGTAAAAACAATTGTAAACATTTCTAACTCTACAGGTTCTAAATTAAGAGGTCCAATTCCTCTACCAACTAAAAAGGAAATTTTTACAATTTTAAGATCACCACATGTTAATAAATCAAGTCGTGAACAATTTGAAAGAAGAACACACAAACGTTTAATTATTTTAGAAAATCCACAACCTAAAACAATGGAAGCTTTAAAACGTTTATCAGTTCCATTTGGTGTTGAAGTTACTTTCAAAATTTAATTCATTAGGAGGCAAGTAAAAAAAATGAAATCATTATTAGGAACAAAAGTTGGCATGACTCAAGTTTTTACAGAAACAGGTAAAGCTGTAGCTGCCACAGTTATCTATGTTGAACCAAATAAGGTTTTAGCTGTTAAAACTAATGAAAAAGACGGCTACAGTGCAATTCAAATTGGTTATGAAACAGTAAAAGAAAAAGCATTAAACAAACCATTATTAGGTCAATTCAAAAAAGCAAATTCAGATCCAAAACGTCACATTAAAGAATTTAGAGATGTTGTTGCTGAAGTTGGTGCTGAATTAACAGTTAGTGAATTTGAACCAGGACAATTAGTTAATGCACAAGCATATACTAAAGGACACGGATTTACAGGTTCAATTAAACGTCATAATTTTAGTATGGGTCCAATGGGCCATGGTGCTGGTTATCCACATCGTTATGTTGGTTCTATTGCTAAAGGACGTGGGGGTAGTCAAGCTCAACGTGTTTTCAAAGGAACTAAATTACCTGGTCATTATGGACATGAATTAGTAACAACTAAAAACTTACTAGTATTAGATGTTAAAGCAAATGAAAATTTAATCCTAATTAAAGGTGCTATTCCAGGACCAAAAGGTTCAATTGTTTTATTAAAATCAGCTAAAAAAGTTGGCCATATCGTAAGCGATCCACAAGTTGTTAACTACTTAGCTAATAAAGCATCATCAAGTGAAGCAAACGAATAAGGAGTTAGTCATGGCAAAAATTAAATTATTATCTATTGATGGTAACTTCGCAAAAGAACTTGAAGTAACCTCTGATCTTTTTGTTGAAGTACCTCACAAACAAGCAATGTTTGACTCAGTGCTTGCTGAAAATGCAGCTGAACGTCAAGGTACACATTCAACCCTAACAAAGGGTGAAGTTCGTGGTGGTGGTAAAAAACCATGAAGACAAAAACACACAGGTAAAGCACGTACAGGTTCAACACGTAACCCACATTGAACAGGTGGGGGTGTGGTATTTGGTCCAAAACCAAATCGTAACTACAATTTAAAAGTTAATGCAAAAGTAAGACTATTAGCTTTCAAATCAGCATTAACTATTAAATTGAATGAAGGAAAAATGCTAGGTTTAGTAGCAAATAGCGATTTAGAAACACCTTCAACTAAAAAAATGGTAAATTTCATTAATAATGCTAACTTAGAAAATCAAAAAGTTTTATTAGTAATAGTCGACAATTTTAGTAATATTAAAAAATCAACAAACAATTTACAAAAAGTTACAACAAAATTATGATACCAAGTTTCAGTTCGTGATTTAATGCATGCAAATGTTGTTGTTGTGGCTGAAGAAGCATTTACAAATTACGCAAGAAAGGTAAGTAAATAATCATGGAATTAACAAGAGTTATTTTACATCCATATACGACAGAAAAAACTTATTCTATTCGTAATAAAAGCGAGCATGAAACATTAACTTTTATTGTTGATAAAAATGCTAATAAATACCAAATTCGTGAAGCATTTATTGCGATTTTTGGTTTAAAACCACTTAAAATTCGTACTACAAATCGTCGTCCTGCAAAAATTCGTACTTCAACAGCTAGACCAGGATATACAAAAGCAAAAAAAATTGCTTATGTAGTAATGCCAGTAGGTGTTAAAGTAGCTGTAAGTAAAGAAGAAGTTGAAGCAGCAAACGCAAAGTAGTTTAAAACAAAATTAAAAATAACAGTCGAAAAGCAAATTAGAAAGAGGCAATTATGGCAGTTAAACGTATTAAAAACCATAGTAGTGGTAAAAGACAAACTGTTGTTGTTGATTACAAATCGATTTTAACAACAAGTAAACCAGAAAAATCATTGCTTGTTACTTTACCTAAAAAAGCAGGTAGAAACAATCAAGGGAAAATTACAATTAGACACCATGGTGGTGGTCATAAACGTAAATATCGTATTATTGACTTTAAAAGAAATAAAGATAATATTTATGGAACTATTAAATCAATTGAATACGATCCAAACAGAACATCATTTATTTCATTAGTTGTTTACGCTGATGGAGAAAAACGTTATATTATTGCTCCTAAAGGAATTAAAGTGGGCGATAAAATTATTTCAGGAAATGAAAACATTGATATTTTATTAGGTAATTCATTACCACTTGAATTTATTCCAGAAGGTACATTAGTTCACAATATTGAACTATCACCAAATGCTGGTGGTCAAATTACAAGAAGTGCTGGTGCTAGTGCACAAATTCTTGGTTTTGATGAAACTAAAAAATATATTTTAGTTAAATTAAACTCAGGCGAAGTTCGTAAATTTAGAAAAGAATGTCGTGCTACAATTGGAACTGTTTCAAATGATGAACACATTCTTGAAAATTTAGGTAAAGCTGGTAAATCACGTCATTTAGGGGTTCGTCCAACAGTTCGTGGTTCTGCAATGAACCCTAATGATCACCCACATGGGGGTGGTGAAGGTCGTTCACCAGTTGGTATGGATGCACCTCGTACACCTTGAGGTAAACGTCACATGGGTGTTAAAACTCGCAACAATAAGAAATCATCAACGAGCATGATTGTTCGTCGTCGTAAATAATATAGAAAGGATTAATCGCGATGTCTAGAAGTTTAAAAAAAGGTGCTTACGCTGATCCTAGTTTATTAAAAAAAGTTGAAGCAGCTAATGCATCAGTTTCTAAAAAACCAATCAAAACATGATCTAGAAGAAGTCAAATTTTTCCAAACTTTGTTGGATTAACATTTGAAGTTCATAATGGTAAAACTTTCTTAAAAGTTTACGTAACAGAAGATATGATTGGTCACAAATTAGGTGAATTTGCACCAACACGTAATTTCAAAAACCATACAGAAGCTAAACGTTAGAAAGAAAGGTAATTTATAGATATGACAAATAAAGTTATTCAAAGAAATATTCATATTTCGCACCGTAAAGCTTCTCTTGTAATTGATTTAGTTCGAAACAAGCCAGTACATGAAGCAATTCGAATTTTAAGTAATACACCAAAGAAGTTTGCTCCAATCGTTTTGAAATTACTTAACTCAGCAATTTCAAACGTTCAACATAACTCTAAAGATATGGATCCAAGTAAACTATATATTTACAAAATTGTAGCTAATCAAGGTCCAACAATGAAAAGAACACTACCACGTGCTAAGGGTTCTGCTGATCAATTATTTAAACGTACAACACATTTAGAAATTGTTTTATCTGATGATGTTAATGAACGTGAAAAAGAATTAGCAGCTATTAAAGCTAAAAAATCAAAAAAACCATTAGCAGTTGAACCGATTGCTAAAGTTGAAACTAAAAAAGTTGCTAAACCAAGCAAAGTAGAAATAAAACCAGTTGAAAAAGACGAAAACGTTGATCCAGAACTTTTAAAACGTGAACAACAAGTTTTAAAAGTTGTTGAAAAAACTGCTTCACAAAAAGAAGAAGAAACTACTGAAACAATTATGATTTCAACATCACCTAAGAATGCACAAGTTTTATTTGATGATTTAGAAAAAAATGTAATCTTTTATAAAACAACACCTATTAATAAAGTTTTACGTGTACTAGTTTATGTAACTAGCCCAACTAAAAAAGTTGTTGGTGAATTTGATTTAGAATCAGTTGAAATTGGTGCAATTTCATCAATTTGAAGAAAATACAGCAAACAATCTGTAATTTCTAAAAAAGAATATGATGCTTACTATGAAGGAAAAGATAAAGCACATGCACTTGTAAGCAAAAAAGCATATAAATACCGTAATCCAAAAGATCTTAGCGAATATAACATGACTAAAGGTCCAAGTGGATTCCAATATTTAAAATAGGGTAAGGGAGATTTTACAAATGGGTCAAAAAGTAAATCCAAATGGACTAAGATTTGGGATCAACAAACAATGACTATCTCGTTGAGTGCCAACTGATCAATTACAAATGGCTAAATGATTAGTTGAAGATGATAAGATTAGAAAATATTTATCAACAAAATATAAAAATGCTGGTATTGATCATGTAGAAATTGAACGTGACCAACAACGGGTAAATGTTTATGTATATGCTGTACAAAGTGGTTTATTAATTGGAACAGAAGCATCAGAAAAGAAATTAATTGAATTAGCAATTAACAAAATCGTTGGTCGTAAACAATTAGTATCTTTAAAAGTAGTTGAAGTTCAAATTCCAGAATTACAAGCTTCATTAATGGCACGTGAAATTGCCGACGCAATTGAAAATAGAGTTTCATTTAGAATTGCTCAAAAAATGGTAATTAAAAAAGTTCTAAAAGCTGGTGCTAGAGGTATTAAAACACATGTTTCAGGTCGTTTAGGTGGCGTGGAAATGGCACGTGAAGAAGGATATACACAAGGTGTAATGACCTTACATACATTACGTGCAGATATTGATTATTCAATGCAAGAAGCACATACAACTTATGGAATTATTGGTGTTAAAGTTTGAATTAATCGTGGCGAATTATTTGGTAACAAATTAGTAAACAGTGTTGCGCATGCTGCAAATAAGGAATTTAGTCGTAGTTCAAAACCAAAAAAAGGATCATTTAATCGTTCATCAAGATCAAAAAATACTAAACCTGCTCCAAAACAAGCAGTTAGCGAATAGGAGATTATAAAATTATGTTACAACCAAAAAGAACAAAATTTCGTAAGCCTCATAAAGTAAGCTACGAAGGTAAAGCTAAAGGAAATAAACAAGTTGATTTTGGTGAATTTGGATTAATGGCACTAGAAGGTGCATGAATTGATGCGCGTCAAATTGAATCAGCACGGATTGCAATTTCAAAACGTTTATTAAAAACTGGTAAAATGTGAATTCGTATTTTCCCACATATGTCATTAACTAAAAAACCATTAGAAGTACGTATGGGTTCTGGTAAAGGTAGTCCAGAAAAATGAGTAGCAGTTGTAAAGGCTGGAACAGTAATGTTTGAAATTGCTAATGTTTCAGAAGAATTAATGTGTGAAGCTTTACGTGCTGCTGGAAATAAGTTACCAATTAAAGTAAAAATTGTTAAGAAGGGAGAAGCTAACTAATGAGTAGTATTGCACAAGATCTTCGTAAAAAAGATAGCTTAGAACTTGAAAAAATTGTTATTGAGCTTAAAGCCAAATTATTAGAACTTCGTTTTGCAGCTGCGAATGGGGAAGCAGAAAAACTTCATACAGCCAAAGAAATTCGTAAAACAATTGCACGTGCATTAACAATTTTAAATGAACGTGAATTAGCAGAAAAATTAAACAACAAGGAGGCTAATAAATAATGGAAAGAAGCCGTCGTAAAGTTTTAGAAGGGTTAGTAGTTTCAGATAAAATGCAAAAAACTGTTGTTGTTAGTGTTGAAACTAAAAGCAAACACCCAATATATAGAAAACTTGTAATTAGTCATAAAAAATACCACGCACACAATGATAATGATGATGCCAAAGTTGGTGACCTTGTTGAAATCACTGAAACTCGTCCATTAAGTGCAACAAAAAATTGAAGAGTTAGCAAAATTTTAGAAAGAGCACGTTAGTGCAACTAAGGAGAAGATTATGATTCAACATATGACAAGACTAAAAGTTGCAGATAACACAGGTGCTAAAGAAGTTGGAGTAATCAAAGTGCTTGGTGGCTCTAAAAAACGTTATGCATCAGTGGGCGATATTGTAGTTGTCTCAGTTAAAAAAGCAACACCTGCTGGTTTAATTGCTAAAGGACAAATGGCCAAAGCTGTAATTGTTAGAACAAAAAAATCAATTCGTCGTGAGTCTGGTTTATTAATTAGATTTGACGAAAACGCATGTGTACTTATTAAAGAGGATAAGACACCAAGAGGATCACGTATTTTTGGTCCTGTAGCAAGAGAAATTCGTGATCGTGGATACACAAAAATTGCTTCTCTTGCTCCTGAAGTATTATAGGAGGTTAGCAGATAATGAACAGAATTAAAAAAGGCGATACAGTTGTTGTTATTTCAGGTAAAAACAAAAATAAATCTGGCGTAGTTATTCAAGTTAACCCTAAAGAACAAACAGCACTTGTTGAAGGCGTTAATAAAATTAAACGTCATCAAAAGAAAGATCAAACGCATGAACAATCTGGAATTATTGAAAAAGAAGCTCCAATTCGTTTATGTAAATTAGCATTAGTTGATCCTAAAGGAAAAGATAAAGGTAAAGCAACTAAAGTTAAATATTTACTTAAAGATAACAAAAAAGTTCGTGTAGCACGTAAAAGTGGTTCAGAACTTGATGTAAATAAAAAATAAAAAGGAAGGATAAGTTATGGCATTCTTAAAAGATTTATATAAAAATAAAGTTGCTAAAGATTTGCAAAAAGAATTTGCTTATTCTTCTGTAATGCAAATTCCTAAAATTGAAAAAGTAGTTATTAACGCAGGAATTGGAAATGCTGTTGCTGATAAAAAACATTTAGAAGCAGCTATTTCAGAATTAACTTTGATTACAGGACAAAGACCTGTTGAAACAAAAGCTAAAAAATCAATTGCAACATTTAAATTACGTGCTGGTCAAAGTATTGGTGCTAAAGTTACATTACGTGGCGATCGTATGTGAGCATTTATTGAAACTTTATTTAATATTGCTTTACCTCGTGTACGTGATTTTAAAGGAATTTCAAATAATTCGTTTGATAACCAAGGTAACTACACTTTAGGTATTAAAGAACAAATTATTTTCCCTCAAGTTGTTTATGATGATGTTAAATCAGTGCGTGGTTTTGATGTAACATTTGTTACAACTGCAAAAACAGCCCAAGAAGCTAAAGCATTACTAGTGGGACTAGGTGCTCCATTTCAAAAAGTAAGAGGAGACAAATAATTTATGGCGAAAAAATCATTAATTGCTAAGCAAAAAAAACATCAAAAATTTGCAGTTCGTGAATACACTCGTTGCGTAAGATGTGGTCGTCCACATGCTGTTAATCGTAAATTTGGTGTATGTCGTCTTTGCTTTAGAGATTTAGCTTACGCTGGTGCAATTCCAGGAATTAAGAAAGCATCTTGATAGGAGGATAGATATGTATTTAGATCCAATTGCTGAATTAATTACAAAAATTAATAATGGTCGTAAGGCCCATAAAGCTGAAGTTAGTTTTGCAACTTCAAAGCTTAAAACAGCGATTTTAGAATTATTGGTTAAAGAAGGATATATCAAATCTTATGATATTCGTCCAACTGAAAACAATAAATCTGAAACAGTTGTTAAACTAAAATACAAAAACCAAACAACATCTTCTATCAATGGTTTTAAACAAATTTCAAAACCAGGATTAAGAATTTATTCAACACACTTAAATTTACCAAAAGTATTAAATGGTTTAGGAATTGCTATCATCACAACTTCAAAAGGTGTTATGTCAGATAAACAAGCAAGAAAGGAAAATGTAGGCGGGGAAGTAATCGCTTACGTTTGATAAGAATATTTATGTCAAGAATTGGAAATCGTAAATTAACTATTCCAGCTAATGTAAATGTTAGTGTTGAATCTGGTAAAGTACATATTGTTAGTCAAACTGCAAAATTAAGTGTTGATTTTCCAGTAAATCTAATTAGTGTAGATGTTGTTGATAATACTGTTAAAGTTAGTCGTGCAAACGATGAAAAACAAACAAAAATGTTTCATGGAACAGTAAATGCAAATATTGCTAATGCTTTAGTTGGTGTAACTACTGGTTGAAAGAAAGAATTAGAAGTTAAAGGGGTAGGTTTTAGAGCAAAAGTTGAAGGTTCAAAACTTAATTTAGGATTAGGTTTTTCTCACCCTTTATTAATTCAAATTCCAACAGGTTTAAAAGTTGAAACACCATCAGCTACTGAAATTAGTATTAATGGAGCAGATAAAGCTGCTGTTGGTGCTTTTGCAGCAGTAGTACGTGCTTATCGTAAACCAGAACCTTACAAAGGTAAAGGTGTGATGTATAAGGGCGAAAGAATTGTGCGTAAAGCAGGTAAAACTGCAGATAAGAAAAAATAGAAAGGTCATTCATAAAGTATGAAAAGAATTAATTTTAGTCGTGCTAAACAACGTGCACTTCGTGCTAAACGTCTTCATGTTAAAATTCGTAACCTTCAATTAGCTGCAAACAAACCTGTTTTAGTTATTACAAAAACAAATGCACACATTTGAGCACAATTAATTTGCTATAACAAAAATATTACTTTAGCTTCATCATCATCTGTACAATTAGATTTACAAAATGGTAATAAAGATAATGCACGTTTAGTAGGTGTTGATATTGCTAAAAAAGCTTTAGCACAAGGCTTTAAACAAGTTATTTTTAACAAAAATGGTGCTAAATACCATGGGCGTATTAAAGCATTAGCAGATGCAGCGCGTGAAGCTGGATTAGAATTTTAAAAAGGAGCAGTTATAATATGGAAAACAATGTTAAAAAAGAAACTATTGTAGATAGTGAAAAAGTTGAAAAGCAACAACTAGTAACTGCACCAGTAGTTAATAAAAAAGAAAATACAGAACCAAAAGCAAAAACATTTAAACGTGAAACAACAACATCAAATTTTGAAGAACGTGTAGTGAAAATTAAACGAATTTCAAAAACAACAAAAGGTGGACGTATGATGCGTTTTAGTGCTTTAGTTGTTATTGGTGATAAAAATGGAACTGTTGGTTTTGGTATGGGTAAATCAATTGAAGTTCCAGATGCAATCAAAAAAGCGATTAAAAACGCAAACAATAATTTAATTAAAGTTAAGCAAACAAAAAAAGGTTCAATTTACCATGATGTAAATGGTCGTCATGGAGCTGCTAAAGTAATGTTACTACCTGCTCCTGAAGGTACTGGAATTATTGCTGGTGGACCTGTACGTGCTGTGGTTGAATTAGCTGGATTTACTGATATTTATACAAAATCACGTGGTGCTAATGCGCCAATGAATGTTATTCGTGCCACAATCAATGGGTTATTACAACAATTAACACCAAAAGAAATTGCACGTTTACGTGACAAAAGTTTAAGAGAAATATAGTGATTAGGAGTTTGTCATGCAATTACATAATTTAGAATATAAAAAAGGTTCAAGAAACCACAAAGAAAAACGTGTTGGTCGTGGTCATGGTTCTGGTTTAGGAAAAACTTCTGGGCGTGGACAAGATGGACAAAAAGCACGTAAATCAGGAATGGTACGTTTAGCTTTTGAAGGAGGACAAACACCACTTTATCGTCGTGTGCCAAAAGTTGGTTTTAACAACGATCGTTTTGCAAATAAATATAACGTTGTAACTTTAATTTCATTAGTAAAATATGAAACAAAAGAATTAACAGTTGAATTTATGTATGCAAATAAAATTGCTAAAAATGAAGATTTACCAATTAAGGTTATAGGTAATGCAGTTTTACCAAGTGGCACAGTAGTAAGTGCTCACAAATTTAGTAAAGGCGCATTGGAATCAATTAGTAATTCAAAAGCAAAAGCACAAATTTTAGAGTAATAAAAGTTTATAATATATAATTTATTACTAAACCAAAATAAGGGGATTAACAATGACTAACAAACAGAAGAAAAAAAATGCTTTTAGACAGTTGTTAATGATCTTTAAAAATAAAAAAGTTTTAGTTGCATTAATTGTAACTTTAAGTATATTAATCTTGTTTAGAATTGGATCTGTTATCCCAATGCCATACATTAAGTTAAATGGTAATTTTGGCAATCAAGGTTCATTCTTTTCAATTATTAACTTATTAGGCGGGGGAGGGCTATCACAATTTTCACTCTTCGCAATTGGGATAGGACCTTATATTACCGCTCAAATTATTATGCAACTTTTATCAAGTGAGCTTGTGCCACCACTCGCCAAATTATCCAAATCAGGTGAGCGTGGTCGTAAAAAAATTGAAGTGATTACAAGAATCATTACCTTGCCTCTTGCAGTGATGCAAGCTGTCATTATTATTAATTTAATGACAAGAGCAAATGGATTTATTTCAATTGTTTCAAATGCACCATTTGCTATTGGTTCACCATTATTTTATGTAACATACATTTTTTTAATGGTTGGTGGCACTTATATTTCGTTATTTTTAGCTGATCTAATTTCGAAAAAAGGTGTTGGTAATGGAATTACATTATTAATTCTTACAGGAATTGTTGCTTCATTATTTAATCACTTTATTGCAATTTTTTCAAATTTAGGTAGTTTAACAAGCAGTAAAGTTTCACAAATTATCGGTTTCATTTTATACATTTTATTCTATATTATGATCTTAATTGGTGTTGTTTTTGTTAATAATTCAACAAGAAAAATTCCAGTTCAACAAACCGGTCAAGCTCTAATTTTAGATCATGAGAAACTACCATTTCTTCCTATTAAAATTATGACAGCAGGAGTAATGCCAGTGATCTTTGCATCATCTGTTTTAGCCATTCCTGCACAAGTTGCTGAATTTTTAGATAAACAAAGCATGGGTTATTATGTGATTCATAATTACTTCATTGTTGATTCTTGAACGGGTCTAGCAATTTATGTTGTTTTAATTTTATTATTTACATTCTTCTTTTCATATGTACAACTTAATCCACCAAAAATGGCTGAAGATATTAAAAAAGCCGGTCGTTTTATTCCAGGTGTTCAAGTTGGAATGGATACTGAAAAACATATTACAAAAGTAATTTATCGCGTGAATTGAATTGGTGCACCAATTCTTGCTTTTCTAGCATGCTTGCCTCATTTGGTTGCTTTAGTAGCTAAAACAATCAATCATGGAATTCCTGTAATTCAACCATCAACAATTTTTGGAGGAACTTCAATTATTATTATGGTTACAGCAACGCTTGAATTATGAAATGCAATTAAATCAACATCAACTTCAACATCATACGCTTACCAAAGAAAAGAATTAGAAACATCCATTACAGTAAGCGCTGAAAGTGACAAGAGTAGTAAGTCACAAATTTGATAGAGGTATTTATGAAAATCCTTTTAATTGGCCCCCCAGGGAGTGGTAAGGGAAGTGTTAGTGAATTATTAACTAAAAATAATACATTAAAACATGTTTCCACAGGTAATTTATTTCGTGCAATTCTAAAAGAGGATAGCGAATTAGCACGTAAAATTAAAGAGATTAATGTTTCTGGTGGTAAATTAGTACCAGATGAAATTACTAATCAAGTTGCTAAATCAGCAATTGATGAATTAATTAAAAATGAACAATCATTTATTTTAGATGGTTATCCACGCACAATTAATCAAGCCTTAGCATTAGAACAATATTGTAATTTAGATTATATTTTTTATCTAGATATTGATCATCAAGAATTAATGAAACGTTTAACTGGGCGTTGAATGTGCCCAAAATGTGCAGGAATTTATAATATTCATTTTAAAAAACCACAAGTTCATGGTTTATGTGACAATGATCAAGCTACTCTTTATCAACGAGCTGATGATCATGCAGATGCTGTAAGTATTCGATTAGATGAATATGATAAATTAACCCTACCTTTGATTAAGCATTATGAAACTAATCCACGTTTTATTAAAATCAATGCTAATCAACCTATTGAAGATGTTTATAAAAACATTAACAATTATTTAAAACAAAATAAGTAAAAAAAAGTAGATGAAGTTAAAACCATGGTTATTGTTAAAACAGAAAAAGATATTGCTGCTATTAAAGAAGCTGTGCGAATTTGAAAAATTGCTCGTGAAGCAATTTATAAACAAGCTAAAGCAGGCGTTTCATTAAAAGAATTAGACTTATTAGCTAAAGAAGTTATTGAAGCAAATGGTGGAATTGCTGCTTTTCACAACTATTTAGGTTTTAAAGGTCATATTTGTATTTCAGTGAATGAGTGCGTTATTCATGGAGTCCCAACCGATTACGTCTTAAAAGATGGTGATAAGGTAACATTTGATGTTGGTGTTAAATACGATAATCATTATTGTGATGCGGCGTTTACCATTATTATTAACAACTCAAATGTTGAAGCACTAAAAATATCAGAGATTTGTAAAAAATCAATTGACGAAGCCGTTGCAATTATTAAACCAAAAGTAACAACACATGCAATTTCGAATGCAATTCAAAAGTTTATTGAAAAAAACGGTTATTTTGTTCTTCGTGATTTTGCCGGCCACGGTTGTGGTAATGAAATTCATGAAGATCCATTGATTCCTAATTATCGATCTTTATTGTATCGTAATGTTACATTAGAAGAGAATATGGTAATTTGTATTGAACCAATGATTCTAAGTGGATCAAATGCTTATTATATTGATCCAAACGATCAATGATCTGTAAAATCTAAAAATCATCAAATGACTTGTCATTGAGAACATATGATTCTAATTACAAAAGATGGTTGTGAAGTTTTAACAGATTAAGGGATATATTTTAAACAAGGAATAAAAATGGCAGATACAGAAAAATTAAAAATGCTTGGTAAAATTGTTGAAGTTTTACAAGGCGGAAATTTTAGAGTTCAATTAGAAAATGGCATCACGATTATGAGCCACGTATCAGGTAAAATGCGTGTTAATAAAATTAACATTTTACCAGGTGATACTGTTGATGTTGAGTTAAGTCCATACGACCTAACACGTGGAAGAATTACATATCGTCATCGTGATAACTAAAATTAAACGAAAGGAAAACTATAATGAAAGTTAGAGCTTCAGTTAAAGCAATTTGTAAAGATTGCAAAATCGTAAAGCGATCAGGTGTTGTGCGTGTTATTTGTGCTAATTCAAAACACAAACAACGTCAAGGTTAATAAAAAGAAAGGAAGATAAATAATATGGCACGTATTTTAGGGGTAGATATTCCTAATGATAAAAGAGTTGTAATTTCATTAACATACATTTTTGGTATTGGAAAATCTACATCTCAACAAATTTTAAAATTAGCTAATATAGATGAAAATATTCGTGTAAAGGATTTAGCTGATGAACAAATTGCTGAAATTCGTCGAGTTGCTTTAAATTTTGTTAAAGCAAATGGCGAAAAATTACAATTAGAAGGTGATTTACGAAGAACTGTAGCCATGGATATTAAGCGTTTAATGGAAATCGGTTCATATCGTGGAATCCGTCACCGTCGTGGTTTACCAGTGCGTGGACAAAGAACAAAAACAAACGCTCGTACACGTAAAGGACCACGTAAAACAGTTGCTAATAAAAAGATTGAAACTAGATAATAAGAAAGGAATAATCAAACTATGGCAAAGAAGAAAAAACTTAGTTTTACTAATGGAATTGCATATATTCATGCAACTAAAAATAATACGATTATTACTTTGGCTGATGAACAAGGAAGCGTTTTATCTTGAGCATCATCTGGTTCAATCGGTTATAAAGGAACAAAGAAAAAAACTCCATATTCAGCAGGTATTGCTGCTGAAGCTGCAGCAAAAGCTGTGATTGATATGGGATTAAAATCTGTTGAAGTTCATGTTAATGGAACAGGTGCAAGTCGTGATACAGCAATTCGTAGCCTACAAGCAGCAGGTTTAGAAGTAACAAAAATTAAAGATGTTACTCCAATTCCACATAATGGGTGTCGTCCACCTAAAAAACCAAGATAGTAATTTGGTTTTGATTATTGCAAAAGGAGAAATAACATGCGTAAATTTTTAAAATACCAACTTGATGTGCCATCAATTAATAGCGAAGACAAAAATCGTACAGTTGTTAAAATTGCCCCACTTGAAATTGGTTTTGGTGACACATTAGGAAATGCCTTACGTCGAATTTGTTTATCATCAATTCCTGGTGCCTCAATGTTCGCTGTTAAATTTGGGGGATATTCGCATGAATTTCAACCATACGAAGGTGTTAAAGAAGACATTACTCACATCATTTTAAACTTAAAAAATTTAGCAATTAAAATTGATGAATTAATTTATAGTGAAGACTATTTCAACAATTTATTAATTGATAAATGACCAAAAATGAAAATTAATTTCAAAGGACCTGGTGTGATTACAGCTAAAGATATTGTATGTCCAGTAGGTTTTGAAATTGTTAACCAAGACTTATATATTGCAGAAGTAACAAAACCAATTGATGTAGAAATTGAAATTTTTGCAAAAACAGGACGTGGTCGTGTCGATTTTAATACAAATAAGGATTTTGTTTCAACACTACATATTATCGCAACCGATTCAAATTATTCACCAGTTCTACATTACGCTTATAATGTAGAAATGATTAAAGATTCAAAATCATCAATGTCTGAGATTTTAACAATTGACATTGCAACAAATGGTACGATATCAGGAAGCGAAGCAATTGCGATTGCTGCTAAGATTATGCAAGCGCATTTAGAACCAATCGTGAACATTGATAAAACAATTAATGAAATGATTATTATGCGTGAGCGTGAGGAAGAAGAAAAACGTCAAAACGCTTCAATTTCAATTGATGATTTAGATTTAACGGTGCGTGCATATAATGCTTTAAAACAATCAGGTATTAATACCACTGCTGAATTAATTGAATTAACAAAATCACAACTTGAAAAAATTAAGAATTTAGGACGTAAATCAGTTACTGAAATTATTCAAAAATTAACTGAACGTTCACTTGAGTTAAAAAAAGATTAAAACTTTAAAAGAAAGGAATAATTTGATATGAGTTACATTAACAAACCAGGTAAAACACGCGCATGAAGAAAAATGGTGAGTCGTCAACAAGTATCTGATGTTATAAGTCATGGTTCAATTGTGACAACAAAAACAAAAGCAAAAGAATCACAACGTCATGTTGATCATTTAATCACTCTTGCTAAGAAAAACACACTTGCTTCACGTCGTGCAGCAGCTGCGATTTTATTAGGAACAAATCAACACTCAGCGGATGATTTATTACGTAAATTATTTAACGAATTAGGTCCTAAGTATGCTAATCGTGCTGGTGGATACACTCGTGTTATTAAACTTGGTAATCGTCCAGGAGATAATACAGAAGAAGCTGTTTTACAATTAGTTTAGTAAATATTTAATAAAATAGATCAATAAAAACCAGTTTTACATTTCGTTTAACTGGTTTTTTACTATATAAAATATAAATTAATTTATTTACAAACAAAAAACGAAATAAAATTTTAATTATTTTTTATTATATGTTAATTGATTTGTTCCACCTTTTTTTAAAAAAATGATAAACAATAATATTTACAAGTAATTTTGTAATTTTGGTCATATATTTTTAATATATTTTATTATTAAGATATACAAAAGTTAGGTTTTATAGACTAATGAATAAACAAAAAAGAAAAATCTCAGTATTAACTTCAATATCATTATTTTTAATTATTAGTTTAAGTATTGGGTTAGGTTTGGGATTAATAAAAAATAATGAAAATTCCATCACTTCTAAAGTTCAAAAAAGTAGCAATTTTTTTATTAATGATTTATCAAAACAATTGTTTTTAGATGTTTTTAATCAAGATAAATTAGAATTAAATGCTTATATGTATGATCAAAATAATGTATCAGATCAACAATTATTAGATTTAAAATTTGCTTTAACATTATTAAATCCTAACAGATTAGATATTAATGATGACGATACAATTGCCATTATGAAAAATAATGCTAAAAAAATTATTTTTCAAAGTTTATCTAATAATTGATACTGATTATTTACCAATTTAAAGAGTTTAGAATTTTCTTACACTCCTTATAAACCTAATTTTTATGCCCCCTATATAAATGAAAAACAAGATTTTACTAAATCATCAATTTTTGACAAAGTTATTAATATTAATAAACCCTTAACAAATTACATAAAAATTAAGCAAAAATTATTCGATAAAGAAAATTATCGTAGTGTTGATGTTTATTATTTGCAATTTGGTGATCACTTAATCTTACGTTTATTAAAATTTAATAAAAAAAATCATTTTGATGTTCGATTTGATACAGATCTTTTTCAATTCAATAAACCAATTATAAATTTGGAATTAATAGCAAAAGAAATGCAAACAAGTATTGATTATCATCGAGAAGATAATTTTAAAAAAGATATTTTAGATTATAAAAAAAATTATGAAGATTATTTATATTATGATGAAAAGCTTAACTCAAGTATTTATCAACAGGGATTAGATAAAATAAATAAAGAATATTATGAAGTCAATAATTTTAATTTTTTTAATACTAAAAAACCACTTTTTCAAAAATTTAGTTATCAAATGTTTTTAGAATTAAAAGATAAGTTGGGTTTTAAACGTTATGTTTTAAGAAATATTGATCAAAAAGAAGTTAGAGCACGTGATCATTTTAATGACAATGAAAAAAAACAACAAAATAAGAATTATTTTAGATCTTTTGATAAAGAATTGAATTATTTTCAAAAATATATTGATCATAATTTAGATATTAATATTAATAAAATTTCAAAGGAAGCATATATTACACAAAAAATAATTAAAAATCTTTTATTAGATGCTTTCAATAATAATGATGTTCAAATTACAAAATATCTAATTCAACAAAATGATAAGGAATACCAAAATAAATTAATTAAACAATTAATAGAATTTAGTAATTATTTTAAAAACCAAAAAACACCAAGAGATTCTAATGAATATAAGCAAAAATTAAATGATTATGAAAAAATATTTTCACAAAATTGGTATTTTATTTTAATGAATTTAAAGCACTTTGAATTAAGTTTTAATAATTGATTTACTTTACCAAAACAAATAAATCCTATTACTAAAGAGATGTTAGGACCAAGTGAAAAATATTTAAAAAGGTTAAAAAAACTTAAACCATATGAGGATTATTATTTCATTAATAATTATTTAGAAAAAAAACAAGAAGGAGATACTTCAATGTTGATTGGTTCATTTAAGGATTTATATATTACAAAAAATCAATCTATTTTTAATATTAAGATCGATTTTTCAAGTAGTGAAAACAGATTAAGTTTAAATCCTTTGATTTATTTTTTTCCTAAAACTAAAAATAAATTATCAGTTGATTTAATCACATCAATTTTTCATCAAGCTATATTTCACCAAAATAAAGAATACTATCAATTGTTTGAAACTGATATGATTGATAAATATGAATATGGTATCCCAGCCCAAATGTTACTTTTATGAAGGGAATAGTAATTAATGTTTATTTTTTTTAAAAAAAATCATCTACTAAAATTAAGTTTATTTTTTATTCCTCTAACATTAATTTCAATCCTTATCACCTCATGTAGCACATCAAATTTAAAAAAACAACAGATATTAACAAATAATGAAATTAGTTTTTTTATGGATAAATCCCAAAATTGTTATGTTGGTTTAATTGTTGATCAGACATTAAAAAATGATAAAAAAACTATTTTTAACGTTAGTTTAATTGATGAAAAAAATACGAAGTTAAATTTATTAGTTAAAGATTATCAAATTAAAGATAACAAAATTTTAATTAGATTACCGCGTTTAATAAAACTTAATGATCATTTAATTATTACAAGCAATAATGCTTCATTCATACCTATTAGAAAAATAATTAATATTGATAATTTAATTGATTTGAATATAATCAGAACAAATAATCAAACACAAGATTCTTGAATAGCTTTTTTAAATAACAAAGTGATTAATAATCTTTTAATAACTATTTTTCCTAATAAAAAAGACCGTGATGAATATATTAAATCACAACAAGAAATTAAAATAGAATATGCTCATGAAATAGCGAATTGATTAAATTATTATAACACAGTGCAAAATGATGCTAATAAAGCAGCAGTATTTAATGAAAATACAGCTAGAAGTAAACTTAATAACAATCTTTTTAATAATGTATCATTAAGCAATCCATTTGCTTATGAACAAGCACGTAAGTTTCACAATACATTATTTAATAAAAATTGATTGTGATTTTTATTTAATTTAAAAAGACAAATTTTCATGTTATATCCTGATGATAATTTATTTCAAGAATCGAGCGAGGAAACAGCAGAAGGATTAAAAGATTCAAAAATCAATAATCGTAGTTCTTTTTATCGTGCTCAATCGAATGAATTTATTGATGGGTTATATGTTGTAGAAAGTTCTAATTTAAATAAAAAACCAGAAGAATTAGATGAATATACTGATTTTAGTAAAACAGCTAAATTTACTCTGTTGAATAATGAAGGTTTTGTTTTTACAATCTCCATTGAAGAAAATTATGATAAAACCAAAAAACTAATTTCGCGTAAAGTTAGTTTGTTGCCATGAATTAAAACTTTACCAAGATTAATGTTTAATGATCATGTAATAAAACAAGAATTTAATTTAGCTAATTATACTGAAAATTCATACGATTACAATGCTGTTAATTTTCATTCACCTCTTGAAATTAAAGTTTATGAAGATCAATTTGGCGGAAAAGCAATTCGTTTTTCATTTGTTGATATTGATCCTAATTAATATGAATTAGAAGTGTTATTTTTATTTCTTTTTATTATAATTTAATTAGAAAAGAAGTATAAAAATATGAACAATTACATACAGCAAGTTATTGATTATGTTAATGATAAAACCTATAAAAATAAGACATATTTTATAAAGAAATATGCATTTGCTTTTTTGACTTCATTAATAATTTTTATTTTTTTATTAGTATTTGGTTTATCAAATTATTTTAATCCTTTGGTTAGTTTAGTTGGTGTTGTATTAATTTTTAGTGTTTTAGTTGTCTTTTGTATTCATATAAAAATTATCAATAATTATAGTTTAAATTTATTAAAATGACTACGTAATCAAAAAGAATTATCTTATAAGTATATTATTAATATTCTTGATTTTATTTTAAATAAACAATTATATTTAAACTTAACAATTATAGAAGTCCAACAAATTTGCATAGATTTGGAAGAATTTAAAAAAACAAATGTCATTTAAAACAATTGCATATACCCAAAAAAATATAAAAATTATTAAAAAATCAAAATTTATAACGATCGTTGAACCTATTAGCGATAAAAATGAAGTTCAATCATTATTTAAAAAATATAAAGAACAATATTACGATGCAACGCACATTTGTTTTGCTTATGTGATAGGTCGTGAATTTAATTATATGAAAGCATATGATGATGGTGAACCAAATGGAACAGCTGGAATACCTATTTTAAATATCATTAAGATCAAATCATTAACCAATGTGATTATATTTGTTGTACGTTATTTTGGTGGCATTAAATTAGGCACTGGAGGATTAATTCGTGCTTATTCACAATGTGCTAAAGAAATTATAGATAACTGTGAAATAATAATTTTACAAAAATATTTTTACTATAAAGTTGTTTATAAGCTAAAAAATACTAAATTTGCAAAGCAAATTTTAACATCAATTAGTTATGAAAAACTAATAGAGAGTTATGAAGATGATAGAGTTATTTTAAAAATCCGAATTAACACCAAAATTAATATATTTATGTCTAAGATTGAATTTATTTTTATAAAACATGATTATTAATAATGTTTTACTCTAAAGGATTAGTTAACGCGTATTTTTTACATATTTCACCAATTGTTCTCTCTATTCTAAAAAACAATCTTGCTGTATCATATTCATTTTTTAAATGATTAGGAACTTGTTTTGTTAATATAAACATTTCATCACTATTTTTAATTTTTTCACCTTTATCATCTGTTTTATAGTCAGAATAATAATAAAAATTATCTCTTTTTTGATTAAAAGGTTCATAGATATTAGTGTAATGATATTTAAAATTATAAAGTTCTTCTAATTTTGAAACATCGTTTGCTGAATCACTTTTTGATTTAATTTGATCAATAATAGGTTTAATTATCTTTTCTTTATAATATTGAAAAACATCTGGTAATTGATTTAAATAGTAATTAGATCAATACATTAATTCACCAAATTCTTCTTGGGGATAAAAACCATAACCAACGGCTTTAATATATGGAACAATTTGCTCAACATCATCTTTGACAACGCTATCATAAAATCTAGAAAGTTTTTGATTAGCATTACGTGCTTTTTTCTCAATTTCGTTTGTTAAATCTTTTAATTTTTTAATTAAGAGGTAGTATTTACCTTTTTTTGAAAGAGAATTAGGATCTATTGATGGTTGTCAATTATCTAACATTGAAGTTTTGGACTTAATAGTAAAAAATAGACCCGAAAAATTGGCTGCGTCTAAACTATCTCTAATAAGTGCTTGACCATATAATTTTCATTTTTCTATTAATTGTTTTAATTCTAGAGATTTAGTGTTTATAAATTTTTGTTTTGCTAAATCTTCAATTTTTAATGCTAATCCATATCAAATAGCAACATTAGTATTATCCATTAAAGTTTGATATATAAACTTTTTACCTAAAATATCTTCTTTTCATGTATCTCAAAAATCTTGATTATTTTTGTGGCCATTTTGTTTAATATATTGTGAAACTTTTTTATGTTCTTGGATTTCTCTTTTAATATTATCGATTTTATTTTCAATTAATTTTCTGTTTAATATACTCTGTAGTACTGAAGAAATATTTTCATGAAGTTGATTAGCTTCATGAAATAATTCCATAATTTCTCTATTGTATAACTTATAATATTCTTCACCATTATCAATTTTTTCAAAATAATTATGTTTTAAATTTTTAACATCAGATTTAATATTTTTGATTTTATTAGCTTCATCATTAATTGTTTTTTTATAAAGACCATCGTATGATTCAATCATTTTTTTATATCTTAATTTGATATCATCAATATTTTCATTATCAGGAATTTGATTATAATTAGTTAAAATTTCTTCAATATTATTTTTGATATGATCGATATCATGAATAATTTTGTTTTTATTTTTAAAATTTAAAAAATCGGCTTGTCTTTTTTTTATTAATTTAACAAAGTTATCAATTTTAATTAATCATGATTCATATTCTTGTGTTTTTTCTTTTAAAAGATAATTAAAATCTTTTATTAATTTATATAATTCATAATAAGTAAATGCTAATAATTCATTCTCACTATTTTCATTTAAATTTAAATTCACCAAGTAATTAATATAATTATTGATTATATCTGCTTTATTAAGAAGATAATTGTATTTATGATTATTTTTAAGAACAAAATCAACTTTAGAACTAGCTTCTTCTTTAATTTTCAAATAAAGATTAAAGAAATTTTTATTGTCTTCTTTTTTAATTTTTGTTAATGAGCAACTCAAAATTATAGGAATTGGTGTAACTATAACTCCTATAAATGATAATAAAAAAGTTCTTTTAAAATTTATTTTCATAATGTAAAATATCCATTAGTTTTAATATCATATATATATATATATATATTAGTTCTTTATATAAATACAGATATTGACTAATATGAATTATATTATATTTCTACACAAAAATTCAATTTAAATGTTTATTTAAAGTTAGGTATGAATTTAATAAAATCATTTACCTTTTTAAAAAACTAACTTTATTATTAAAGTTAGTTAGGTTTATTTTTATATTTCTTTATGGCATTTATTTATTAAAATTTTTCTTTTAATAATTAAATTATTCAACAATGCCTTGTAATACTGTTTTTCATAACTAATTTTTGTATTATTAATTTTTAAATTTGTTGATTGTATTTCTTGGTTTAATAATGCATAAGTTTTATTTTTATAATCAAAAGTACTACGTAGTTTTAAATATCTAAAATATTTGATAATTTCAAAAATACTTAAAGGTATTGCAAAAAGAACAAATGGAAGAATATTAAATCACTCATAACCGTTTATGTTGTATGGATTCATATTGAATATTTGATTTAAATGAGGAGTATAAGCGATAACAATAATCATAACTATTGATATTAATATTGAATAAACAACGTATTTACAGTTTTCTCAAAAATTAACGAATACTGAATGATTTGTTCTTAAGCTTAATGCATTAAACGTTAAGCTCATTCCCATAAAAACAAATGTTAAAGACGAACCAGCCATAATCGCTGTTTTTATGGTTAAAATTTTTTCATGAATAATATTGGAAATTTCGTGATTTTGCCCAAATCAACTTCATGTGTCTTGATATATCTTTTCAAGATTAGTAAGTTCTTTAAACTCGTTTCTCATTAGCATAAAGTCAAAATTAAAGTGTGAAGAAGCTCCTAAATAAAATAATAAAATAGAAATTAAACCAACAAAAAAACCATTGGTAAAAATAGTAGCTCACATTCTTTTATTAACAATCGGTGCTGATTTTTTTAATGGTTTGTGTCGCATTAAATTAACATCCGCTAAATGTAACCCTAATGCTATTCCAGGTAAAGTTTCAGCAATTACATTAATTCATAATATTTGTAGTGATGACATAGGATTAATTCCTAGAATAATAATTCCAAATATTAATGAAACTAATCCAGATAAATTAGTGATCAATAATAAAACAATAATACGTTTAATGTTGTCCATAATAGAACGTCCTAAACTAACAGCATTAATAATTGTTGCGAAATTATCATCAATTAATATCATATCACTTGATGCTTTTGAAACATCAGTTCCAGTTATACCCATTGCGCATCCAATGTCAGCAGCTTTTAGTGCAGGTGCATCATTGACACCATCTCCAGTCATACTTACTACTTTATCATGGTGCTGTCAAGCTTTAACAATACGGATTTTATCTGTTGGAGAAACTCGGGCATAAACACTATAACGATCAATATTATTTATTAATTCATTATCACTCATCATATTCAATTCATTACCATTCAAAACTTCTTGTCCTTCGTTTAAAATACCAACTTGTTTAGCAATGGCTGATGCTGTATTAATATGATCGCCAGTAATCATTATCGGACGGATTCCAGCTTTAATAGCAATTTTTACTGCCTCTTGTGTTTCAGGACGAGGCGGATCTATCATACCGATTAGACCAACAAATTCTAAACATTTTTCAAGTTCATCTTGGTTAAAATTAGTTGGTATAGATGATAGTTTTTTAATTGCAACACATAAAATTCTTAAAGCTTGTTTACTTAAAAAATCATTTGCTTCATAAACATCTTTTGTAATTGGATTTTGAACAATTTTGTCAATAGCATCAATTGCTCCTTTAGTAATTAAATAATAATCACTTTGATGTTTTACAACAACACTCATTAATTTTCGAGTTGAATCAAATGGAAATTCATGAATTCTAATAAATTCTTGATCTAATTTATATTTATCTATGTTTAATTTTAAAGCAGCTTCAATGATACAAGTTTCTGTAGGGTCGCCAATAAACTCATATTCTTGTTTTTTTTCATTAAAATTTTTAATTCCATTATTTGCTATAGTAGCTCATTTAATTAAATTAAAAACACTCTCATTATCAATTAAATCTGTTATTTTTAATTTATTAGTGAAAACTTTAGTAACAGTCATTTTGTTTTGTGTTAAGGTTCCTGTTTTATCAGAACAAATAACATTAGCACTTCCTAGAGTTTCAACAGCTGGCAAACGTTTAATTAAAGCATTTTGTTTTGCCATTTCTTTAATACCGAATGATAAAACAATTGTTACAATTGCCACAATTCCTTCAGGAATAGCTGCTATAGATAATGAAATAGCCATTATTAAAGCGGGATGTCAACTAATATTTCAATTTCCATCTCCAATTAAATAAATATAAATAATAAATACTGCAATACATAAAATAGAAGCAAATAGACCTATAATTTTAGATAACTTATTAATTTTTTTTTGTAAAGGCGTTAATTTAGTTTTTTGATCATTAATTAATTTGGCTATTTTACCAATTTCAGTATTCATACCGATATTAGTAACAATTGCTTTTGCTGTTCCATTAGTAATATATGTTCCAGAAAAAATTTGATTTAAACAGTCGCCAATTACTGTATTTTTTTTAACAATTGCCTTGGCATTTTTTACAACTGGTAAAGATTCTCCTGTTAAAATAGCTTCATCTACTTCAAGTGATGAGCTGTTCACTAATAAAGCATCAGCTGGGACTTGATCACCAGCTTCTAAAATCAAAACATCACCCATTACAACTTGGTTAGAATTAATAATTCTAATTTGATTATTACGTAATACTTTGACCTGATGAGACGCTAATTTAGACAATGCATCCGTATTTTTTTCAGCTTTCACTTCTTGAATGGTACCAAAAATTGCATTTAACAATAAAATAAAACATATTATTCCAAATTCTACATATTGAACAACTAATTCAGCTGTATCATGAGGAGGTTTAATAATCACTAAAGCTAAAGTAACAGCAGCAGCGATTACAAGTAAAATAACCATAAAATCTAAAAACTGCTTTAAAAATTTTGTAATAATGTGGGATTTTTTTTTCTTTTTGATTTCATTAAAACCATAAATTTGACGACTTTTTAAAACCTGCTCATCATTTAGACCTGTTGATGGATTTATATTTTCTGGATCAAATGACATAAAGGAGTCGGTTTGATTTTCATTATTTTTTTTCACTTAAAACCTCCATTGGTTTTTAAATTAAACTATAAGAAAATTATTAAAATTTAACTTAGTCATTTATTATAATATATAACTGTATGACTAAATATTATATTTAATAATGTCAATTAAGTAAATAAAAAGTAATTTTTTTTAGGGGTAGTTATGAATTTTTCAAGTTTTATATTTAAAGTTTCTGATGTTTTTAAATCAGTTATTCATGAAGCTTCAGATGTTGTTACTAAAGCAGATTTAGATAATGCTAATGCACATACACATTCATTAGCAGTTGGCTTAGGTATTGGAATTGTTTTATTTTTAATCGCAGGACTTATTATCGGTTATTTTATTTCGATGAAAATTATGAAACGCCAATTGAAGAAGAATCCACCAATTTCAAAGGATACAATTCGTATGATTTATCAACAAGTAGGACGTAAGCCAAGTGAGTCACAAATTAATGAGATTTATAATCGGGCAGTAAAACAAAAATAAATATATGGCAAAATTTATTAAATCTGCACACTATTTTGACCAATATCCAATTGATAAGCAGTTTGAAATATGTGTAATTGGTAGATCAAATGTCGGTAAGTCAAGTTTAATTAATGCATTAGCAAATAAAAAAATTGCAAGGACTTCAAATACGCCTGGAAGAACACAGTTAGTAAATTTTTTTGATTTCAATAATTTTAGATTGGTTGATTTACCGGGATATGGGTTTGCAAAAGTTAGTAAAGAAAAACAAACGGATTTAGCAGCCATTATTGATCAATATTTAGGGTATCGCCAAAACTTGTGTGCTGTTTTTCAAATTTGCGATATTAACGTATTAACAAATGATGATGTAGAAATGAGTCGATATTTTGAGAATCAAAAATACGCTCATTTTGTTGTTTTAAATAAATTAGATAAAGTTAATAAATCATATTTTAATAATAACAAGCATAAAATAGCTAAATTTTTAAATATTAGCACCGATCGTTTATTATGTGTTTCTGCACAAAATAATATAAATATTATCACATTATTTGCGTTAATGAAAAAAGTTGTAATCCAAGCAAAACAAGAAAAAATACTTTCAAAAAAAGAGGAGAAGATGAGTAGTGAAGAAGAAATTAAGTAAGAAGTATTCATTCAAAGAGGTTGAAGCTAATAAATTATTGTTTTGACAAGAAAATAATTTATTTAAAGCTCAAATAAATAGTACAAAAAAACCTTTCACAATTGTTTTGCCCCCTCCTAATGTTACAGGTCATTTACATATTGGACATGCCTATGATTTTACCTTATCTGATATTTTAATGCGTTATAAGAAACTAAAAGGATATGATTCCTTTATTATTCCTGGGACAGACCATGCAGGGATCGCAACACAGACAAAATTTGAAAAAAATTTAAAAGTAAACGCACAAACCAATCGTTTCAATTTAGGGCGAGAACTGTTTTTAGAAAAGTTAAAAATTTGAAAAGATGAACAAATTAATTATATTCATAAACAATGAAATGCTTTAGGTTTGGGTTTAGATTATAGTAATTATTTATTTACATTAGATCCAATAGTTGTTCAAACAGTGCGCGAAGTTTTTGTAAAAATGTTTAATGAACATATTATTTATCGTGATAAAAAATTAGTTAATTGAGATATTCAATTAAAAACGGCAATTAGTAATATTGAAGTGATTCATAAAGAAGTTGAACAAAAACTGTATTATATAAAATATTTAAGTCAAGATCAAAAGGATTTTGTTGTTGTAGCCACTTCACGCCCTGAAACTATGTTTGGTGATAAATATTTAGTTATAAATCCTAAAGATAAACGTTATTTTCATTTACATAATAAAATTTTTATTAATCCAATTAATAATATAGAAATGACTGTTATTTTGGATGATTATATTGATATTGAATTTGGAACAGGAGTGATGAAGTGTACGCCTGCTCACGATTTTAATGACTATGAATTAGCTAAAAAACATAATTTAGAAATTATTAATATTATGAACGCTGATGGTACTTTAAATGAAAAATGTGGGGAATTTAAGGGTTTAGATCGATTAGAAGCTCGTTCTTTAATAATTGATAAATTACAGAAAAATAATCATTTATTAAAAGTAGAATCTTATCGAACCAGCGTTGGTTTTAGTGAGAGAACTAATGAAATTGTTGAACCTTATTTATCACATCAATGATTTATTAAAATGGATAGTTTAATAAAAGATACAATCAAAATGCAAGATGATTGTAATAATAAAGTAGATTTTTATCCAAATCGTTTTAATAAGACTTTACTTACATGATTAAAAAATACAGAAGATTGATGTATTTCAAGGCAATTATGATGAGGTCATCAGATACCTGTTTGATATCATAAAAAAACTAATCAAATTTATTGTGACACTATTCCCCCAAAGGATTTAGAAAATTGAATTCAGGATGAAGATGTTTTAGATACTTGGTTTTCAAGTGGAATGTGACCTTTATTAACGACAAAATGAAATTATAATAGTCATTTTTTTGACCGTTATTTCCCTACATCTTTAATAGTTACAGGTATGGATATTTTATTTTTTTGAGTCTCAAGAATGATGAACTTTAGCCAATATTTGGTTGAAAAAAAACCTTTTAAGGATGTTTTAATTCATGGTTTAATTCGTGATTCACAGGGACGTAAGATGTCAAAATCATTAGGGAATGGTATTGATCCTTTTGACATTATTGATAAATATGGATTAGATGCTATGCGTTTATTTTTTGCATCATGTACAACAATTGGTGAAGATTTAAATTTTTCAACAGAACGATTAGGCGCTAATTGAAATTATTTAAATAAAATTTGAAATATTGCTAAATATATTGAAAATTTAGATGAAATTAACGATAATCTTAATTTTGAAGATGTTGATAAATTTTGTGACGTCAATAAATGAATATTGACTGAATTATCAAAATTAACCTTGGAAATTAATAAAAATATGGATAAATATAATCTAGTTGTTGCTACAAAATATTTATATGATTTTATCTGAAATACTTTTGCGAGTTATTATTTAGAGTACACAAAAGTTTTATTACAAGATTTAACATTAAAAAATGAAACAATTAAAACAATTCGCTATGTATTTAATAAAATTTTAATCATGCTACAGCCTTTTGCTCCTAATATTAGCGAAGAAATTTGATTATGTTTAAATCAAACAAATAATTCAATATTATTACAAGAATATCCAATAATTAATTTTGAGTTTGAAACAATAATTATAGATAAGATTGCGAAAATTATTTTAGAAATTAGAAAGCTACGATTAGAAGAAAATATTAATAATAGAATCAATCTTTGTTTTGAATTAATATCACCCAATGATGCATTTTATAAATCAAAGATAAAATTAGTTAATTTGTTATTAATCTTAGTTAATGCTGAAATAAATGAAATTAAAAAAACAAGTTCTAATAATTATACTTATGAGCTAGTTATTGATGATTTTATTCTTAAAACTTCGTATGAAAAACCGATTGATTATGTTTTTCAAATGAAAAAAGCTAGTGAACAGTTAAACTATTTGGAAAACGAAATTCAACGAGCAACTAATTTATTAAATAATTCAGGTTTTATCAACAAAGCCCCTGCTCAATTAATAATTAAAGAAAAAAATAAATTAATTAATTTAAAAAAAGAACACGCAAATTTATTAAAAACTCTTACTGACTTAAAACAAAAGGTGAAATAATGTTATGTTAAATATTGTTTTATTTGAACCAGAAATTATTTTAAACACTGGTAATATCGCTCGTATGTGTGTAGGTTTTGATGCAAATTTACATCTTGTTAGACCATATGGATTTATTTTAGATAAAAAACGTTTTCACAAAGATTTTATACGTGCCTCAACCAATCATTTAAATGCCTTAAAATTATATGAATATGATGATTTTAATGAATTTATTAATAAAAACAACATTAGTGGTGATGAAATATATTTTTTTACTAGATATGGTACTAAAACTCCGTGCGATTATCAATATCAAAATATAATTCAACATAATGTATATTTAGTTTTTGGTAAAGAATCAACAGGGATAGATCATAATATTTTAAAAAAATATCCTCATAATTGGATCCGTATTCCTACAAGCATTAATTTGAGAAGTTTAAATATTGCTAATAGTGTTGCTATGGGAATCTATGAAGTAGTGCGGCAAAATAATTTTAAAGATTTATTCAAATATGAACCTCATAAGAAATTTGAGTAATTTTTATGCGTTTACGAAATAATGCCAATGCTCCACTTTATTTAAAATCACAACACGAATATATAATTAATGATCCACACCTTTTAAAAGATAATTTAGGAAAAATTTTTAAAAATCCTGAATTGCCTCTTTATATTGAAATTGGAATGGGTAAGGGTGATTTTATTATTGAAAATGCCTTACGAAACCAACAAATTAATTATTTAGGTATTGAAAAATTTCCAACAGTAATTGTTAAAGCTCACAAAAAAGCTTTAAAACATAAATTAGATAATTTAGCAATGATTTGTTTTGACGCAAATAAAATTTTAGAATTATTAAATTCTGAAAGTGTAGATAAAATTTATTTAAATTTTTCTGACCCCTGACCTAAAAAACGTCATGCAAAAAAACGACTGACACATCCGTGTTTTTTAGAGAAGTTTGCAGTTATTTTAAAACAAAATGCTCTTGTTGAATTTAAAACAGATAATGAAAATTTATTTATGTATACTATTTATGATGTATTGCTAAAGGATTTAACAAAGTATGAAATTTTATTTTTAACTTATAATTTATACACACTTGTTAACAATGTTGAATTATTAAAAAATATTCCTACCGAATATGAAAAAAAATTTGTTATGCAGGGCGAGCGTATTAAAAAAGTCAATTTTCGTTTTCTTAAAAATAATCAATAAAAAGATAACTATAATGTTATAATTATATGGTTATATGGCGGTTATGGTGAAGTTGGTTAACACGCCTGATTGTGGCTCAGGTATTTTCGCGGGTTCGAGTCCCGTTAATCGCCCCATTAAAATTAAAAAAACTTAATGTTATAAAAAAACATTAAGTTTTTTCTTTGTATAAATAATAAAGATTTAAAAAGTTAATAATTTAGTAAAATTTTATATATCTTATCTTAATTATTTATAATTTATTATTATGATCAATAAAAACAAAAAATTTCATTTTCAGTATTTTAAAAAAGATAACGATCAATCAATTTCTACTAAAGAAACATTAAAAAGATATTTTAATTTTAGGTCTCAAAGTGTAAAATTGATTATTTTTAATTTAGTTTTAACAGCATTTTTTCTTGCATTATTTTTAATTGGTCGTTTTTTAACTAAAAATTTAGATTTTTTAAATGGTTTTAGTTGACAATTGCAAATGGGAATTTTTGTTTTAGCAATCGTTTGTATTCCCAATTTTTATTATAAATTAATGTATTATATTATTGCGCCGTTAGTGATGTTAGCAATTGGTTTTAGTGCTGAGCCCTTCTTTGGATATTTAATGCCACATTATGGTTTTGGTTTAATTTTATTTACGGATGTCATTTTAATTATTGTTAAAAAGTCATGTCAGTCAATTAAAAAGCAAATATTTTTATCCTATTTATTAATTTTTAGCTTTTCAATCATTGGTTATTTTATTGTTTGAATGGGCTATAGTATTCAAGGAACACTTTTTTACAATACTGCTTTTATTCCTTCTATGATTTATAATTCTTTGGTAACTTTTGGTTCAATGGCAATTAATTTTGTATTATATATAATTTCAATTCCCGCTATTGTTGCCTTAAAAAACAAATATTTATGTAAATTAGTTTAAAATTATAAAAATAATTTTTTACATTATTATTTATTTATAGACAGGAGATGTTTATGTTTGATCAAATCTTTAAAAAAAGGAAACGTCATTTAAACTTTCAAATTTTTATTTTCTTTTTTGTTTTAATATTAATTACTTTTTTGATTTTATGAATTATTTCAGGTAATGTTCAAACAATTATTGATTTTATTAAAAAAAAGATTTATGATGCTAGTCCACAATTAAAATATTTAACAAATATTCAGATCAAACCTTTTAAAAATTTAGAAGAAATTTTTGATTTTATTATTAAAAATAAGAATTTGATTAAAGATTTACAAAACGAACCTGAAATTTTAGATGTTATTAAAAACAATCCACAGATCTTAAAAACACTTAATGATAACCGCGAATTTTTAAAACAAATTGTTGAATATAAGCCATTTGCTGAAATTCTTAGCAAGCAACCTGAAATTTTAAAATTTGTTCAAAATAATAAAGAATTAATTAAACAGATACAAAATTTACCTGAATTATTAGATTTAATTAAAAAACACCAAAATTTAATTGATCACTTAAAAACTAACGAAAAATATATAGAAGTTATTAAAGCTAATCCTCAAATGTTAAAAGATCTCTATCATGTAGATGATAATTTATTTAATATTATAAAGACAAGCTTAGAAAATGATAAAGAAAATAATATTTTAATGTTGGCTATTAAATATTCAGTATTAATTAATAATTTTACTAAGAATTTAACTTTACAAGAATGAAAGCAAATAATAGAATTTTATAAAACAGTTGATTTAAATAAATTTATAGTTGATGATAATTTGTTTAATATTATTAAAAATAATAATCAATTATTTGAAGTTTTTTTAAATAATTATGACTACATAAAAAATCTTGAGGACAATCAAAAAATTATTGATATTATTCAACAAAATCCTGATTTAATTGATGATTTTAAAAAGATTGATTTTGATCTTTTAACACAAAAAAGGAATTTTGTTCGTGCACTAATTAAAATAATTGATTATAAACAACCCAAAGAAATTCAATTCCTTAACAAAAAAGTTTTTTTAGATTTCTTTAAAAATAAATCTGAAATTACCAATGCTTTAAACATCACATCTTTAATTGGATTTGTATTGTTTATAGTAATTTATACAATTTTTATTTTTGCATCTTTTATTTCTCATATTCTAATGTTAAAACAAATTAAAGATTTTAATAAAATTTTTAAAGAAGCTAAATTAAAAAAAATATATCTTATAAGCACTTTATTATTACAATTTTTATTTTTAATTTTATTCATTATTAATTTTATAGTATTAATTATTCTAGTTGTAGATTATAATAAATTGAAAGCAATAGTTTTAAATAGTGGTATAAAAAGAAAAGTGGAGGATAGTGATGCAAGATTGCATTTTTTGTAAAATTTTAAAAGGTGAAATTATTTCAAAAATAATAGATGAAAACGAATTTGCTATAGCAATTTTAGACATTCAACCTGCAAGTGATGGTCATATGTTGATTATTCCTAAAAAACATTATCGCAATTTTTCACTAACTGATCCTATTTATCTAGATGGTATGATGCGTTTAGCAAAAAATATGACATTTGTTTTAGAAGAAGTTTTTCCTAATGTTTTAGGTTTTAATTATTTAATGAATTCAAATTCTAGTGCTGGTCAAGTAATCATGCACACACATTTACACATCATTCCAAAACAAAGTAATGGACGTGGTTTTGTTTTTAAAGCAATCAAAGAAGAAGGCGATATTAGTAATATTGATGAAATTTATAAAAAAATCATTATTAAAACACAAAAATTAAAAAAATCACGTTTAAGTAAGCATTATATGGTTTAATCAAAAAATATGGAACAAATTAGAAATTTAATTGATTTACAAATCAAACTAAAACCAATCCAAAAAGCATTTTATGATGCGTGATCAAAAACATTTATTAATGGAATTGATAATAATAAAGAAACTCATGAACAAAACAAAAAAATTATTTTAGAAATTTATATGATTTTAAAAGTTTTTTTAAACAAAAACCGAGATATTATTTCTAAAATTCCATTAAATCAAGTTAAAAAAATAGCTAATGACATTTTAGAAAAAGAAATTATTTTAGAACAGCCTTTAGTTGATTATTATTACCAAAGTTCATCTTGTTTTATTTTAATACCTTATTTGATTCAAATTCTTTATCAATCTTATCACCTTAATAAACCAATTTATAAAATAATGTGTAAATTTATTATTAGAAATAATTTAGCATTATTTAAAGAATGAGATTTAATTGAACGTCAAACATTGGAAATTATTAAATTAAAAACAAATTTAATTGAAGATAACAATAAGGCAATCAATTTATTTAGTTGTGAACAACGAGAATTACAACACAATCGTTTTGTAAAATTATTTAATAATTTTATTTTAGTGTATTGAACTAAACGAGAAGTTAAATACATAGAGGCAATTCGTTTTTTGATGTATTTTATTTGAATTCCTATCATTTTTATTGTTTTATTAATTTTGATTTTAGGTTTATATTTTGGTTTAAGTAATAGTGAATCCTTAAAAAGTTCAACACAATTCTTACTAGATTTGTTTATTATTAATTAAAATATATCTAAAATAGTTAAATTTAGTAAATTTAAATCATCAATAAGAAGATGATTTTTTTTATTAATACTAACGTGTTTCAATTGATTTTTAAAATATGAATAAGCTTCATAATTAATTTTTTTATTTAGATTTAATCCATCTTTTAACCTTAAACCCATCATTAAAATTTGTTGATATAATTCGTGTTTTGTTAATACTTTTTTAATTAATATAGGGTTTTTTATAGATCCATCAAAAAAATATACAATATTTTTTTCATAGCCATGTGCTCCCCATCCTATTGCGGCCCAGTCTTCAGTTCGTCAATAAGCTAAGTTATGTTTTGCAATATATCTGAAGTTATTTGTTCAATTACATACTTCATAACGATGAAAATTTAATCCTGCAAATTTATTAATTATATAATTCATTTGTTCAATTTCTTTTTCTTCATCGATAGTAATATGGTACTTTTTTAAAAGTGACTGTTCTTTAATTTCTAAACTATAAAAACTAACATGATTAATTTTATGATTTAAAATAAAATTAAAAACATCATCTAAGTCTTTTAATGTTAAGATTGATATGCAAAATAAAAAATCACAAGACACATTATACAATCCGTTTTTATGTAATAAATTAATCGCTTTTTTACTATCTTGATTTGTATGAATAAAATTCATTTGTTTTAACACATCATTATTCATTGATTGAACGTTAAGACTAATGCGATTAATTTTGTATTTTTTGAAGAGATTAATTTGAGTTTGATTTATTAATTCAGGATTACATTCAATGGTATATTCGCAATTATCATCAACATAAGGAGAAATTGTTTTTAATAAAATTTCTAATTCTTGATTGTTTAAACAATTTGGAACACCTCCGCCTAAGTAGATTGTTTTAAATTGCTTTATTTTAAAATTATTAATGTGCATTTTAATATTTTCTAAAAAATTCTTAAAAAATTTTTTTGTTTGAGGAGTTTTTAAAATACGTTTAAAATCAGAAAATATACAAATATAGTTACAAAATGGAATATGAATGTATAAATGTTTAGTTTTTTTGTTAAAAATCATAAAATAGAGATTAATTATGCACTTAATAATCTAAATAATTCAGTAATAACAAAAACTAATAAAACAATTGCTCCGCAAGTTAATATTATTAGAAAAATCGATAAAATAACGCGTTTTTTAAAGTCCATTTTTTTATGTTTTTTTGAATAAATTTGATGCTGATCAAAATCGCTTAAAACGACAGTTTTGGTAACACGGTCTTTTGGGTTAAATGCTTTTGTACTCATTTTAAAAATCCTCTTGATTGCTGAATTATATAAAAAAATAGTTAAACGTAAAACTTATTTAGTCAGCTTGATAATAAAATATCAAACTTGTTATAATTAATAATTATATTATTACTTATCATAAATAAAAATAATTTATTTATATTCTAGGTATCTTGGAGGATTTAAAAGTTAGAATTATGGGATTAAAAAAAGAAAAAAAAGATCGTTCAACGATTCAATTATTATTTGGAACAATTAAAAAACGCAAAATTGCGAACTATTTAATTATTGCTTTTGCCTTTTTAACATTTGCCTTAGCAATTATAGGAAGTTTTGAAGCTATTGCATCAAAATATGCTATTGGCATTGGATCAGGGCTTTCATTATTTATTACGATTGCTTTTAGTGTTTGACGAATTTTAATGATTCATTTAGAAAAAGAATGAAATAAAAGTGAAATTATTTGACAATCCGTTATGGGCGCAATTTTAGTATTATCAATTATTTTTTGAATATGAGCAACAACTACAACAACTATACAATATTCATCACTAAAAGAACATCTTTTATTGTTAAAACAACAAGATTCACGCGCTAATTTTGTTGTTTTAACACAAGCAACAGTGGATGAATTTGGAGTTGCTTTAAAAGGCGGGTTATGAGCTTGAGTTATTACAAGTTGTATTTATATTTTTATCAATACTTTTAATAATATGTTTATGAAAGAAAAAAATTCAAAATAATTATGGGACCAATTATTCCAAATACACGTTTAATTGATTTTAAATTTGATCGAGACTTATTAAATAGTGCCTATACTGCTCATTATTTTATTAAAACATGCAAGATAATTGAATTGCATGCACCAAATCATTCAGTTATTATGCAATTTACACACTTTAGCAAAACACCGATCATGGTGTGTGGAACAAGTGAGGTTTTAGCATTATTAGAGTTTTGTTTATCTAAAAAAGAACTAAAACAATTAAAAATATATTATGTTCCAGATGGACATGTTATTAAACCAAAAGAAGCTTTATTTGTTATCGAAGGGCCATATGAAATTTTTGGCTGATTAGAAAATATTATTGACTCAATTCTAGCACGACGTAGTTCTATAGCAACGAATTGCTATAATGTTTTAAATTTAATTAATGATGAACAAAAAGTAATTTATATGAGTGATCGTTCTGATGATTATAGTTTACAACCATATGATGGGTATGCTGCTGCTGTTGGTGGAATGCAATACTTTGTTACGCAAAAGCAGGTTGAATTTTTAAAAGATATTAATTATGAATGTAAGGTAATTGGCTCAATGCCACATGCTTTAATTCAACAAAACAATGGTCGGATTGATTTAGCATGTGAAATGTTTGCACAAACTTTTCCTAGCGATCCTTTAATTGCTGTTATTGATTATAATAACAATGTTTTAAATGATCTTGAACAACTACGATATATGTTTGATCGTTTGTTTGCAGTACGAATTGATACAGCTAAGGATTTAATTGATGAAAGTTTATTATCGACTTTTAATAATGTTGGTAATCGTGATTTACATGGTTGTAATCCTTATCTTATTGCTTTAGTACGTGAATATTTAGATAATAATGGAGGAAAACATATTAAAATAATTGCTTCAAGTGGGATTGATTTAAATGGTATTAAAAATTTTAATAAACACAATTCTGCAATTGATTTTTATGGAATAGGCACATATTTAAGTCACTTATCAGTTCACATCACTGCTGACTTGGTTTGTTTAGATAATGTTTATGGTGCTAAAGTTGGCCGTAAAATCGCTAAGAATTTTTCTGAAATGATTTTATATTAAAGTTTTTAGAATTTAATGCTGGAAAAGGATTATAATTTTAAATAATCTATTTTTAACAGAAATGTGGGTGAATTTATGTCAAGAGGTGTAAGCGTTGAAGGCGATTTAGAAAAGGCTCTTAAGAAATTTAAACGTATTTCTAATGAAACAAAAAAAGATTCTAAACGTCACGAATATTATTTAAGTCCAAGAATTCGTCGTAAAGAAAAAATTAAAGAGGCTAATAAATATCGTAGTTTCTAAATTAGAAACGCACATGAATCCAACCATTACTTGAATGTTTTGGTTGGTTTTTTCTTTAATATTAAGATTTTTCAGAAGTGCTAGAATTATAAAAATGGTTTATGTTTAAGGAATAAATTAAATATGATTACACAAAAAATAAGTGAAGAATTAAATAAGGCTTTGGCGAAAATGGGTATTCATGATACACAGGAGACAAAGATTCTCGTTGATAAAACTAAAAATATTAAATTTGGTGATTTTTATACTAACATAGCAATGATTTTAAGTAAAAAAAATAATAAGAGTTCTTTAGAAATAGCTAAAGAAATAGCTAATAATTTTGAACAAGATTTGTTTTTAGAAGTTAATTTGCAGCCACCTGGATTTTTAAATTTTAAATTAAAAGCAAAAGATCATGAAAATTTATTGAAACAAATTTATTATGAAAAAGATAGATTTGGTCAATTCTCTAAAAAGAATATCACTTATAATATTGAATATGTTTCAGCTAACCCCACAGGTTATTTGCATATTGCTCACGCTGCTAATGCTATTTATGGTGATATTTTAGCTAATTTATTAAAAATTTATGGATATGATGTAGAAACTGAATATTGAATTAATGATGCTGGAAATCAAATTGATAAATTAGCAATGTCAGTGTTAGTTCGTTATTTACAATTACAAAATATTAATATTCAATTACCAGCAGATGCCTACCATGGGCAAGAAATTCATTTAGTTGCACAAACTTTATACCAAACTTATAAAAATCAATTTATTAATGTACGTTTAAATGAAAAATATGAAATTGATGATGATATTGCTAATCAAGAAATTAAAAATTTTGCAGTTAAATACTTATTAAATGAAATTAAAAACGATTTAGCATCAATTAACACATTCATTGATACTTATACTTCAGAAAATTGAATTCGAAATTCGGGACGTATTTTAGAAGTTTTATCAAAAATAAAACCTTACACTTATACACTAGATGGTGCTTTGTGATTAAAAACAACTACTTTTGGTGATGATAAAGATCGTGTTTTAATTAAATCTGATGGTTCATATACATATTTCACACCAGATATTGCTTATCATGACTATAAATTTAATAAGACAAATACAACTAAATTAATTGATGTGTGGGGTACAGATCATTTAGGTTATATTGCGCGTTTAAAAGCGGCAATGAATGCTTTAGGTTATGATCCAAATAATTTAGAAATTGTTTGTGCTCAAGTAATGAAATTAGTTAAAAATAATCAAGAATTTAAACTTTCAAAAAGGAGCGGACAATCATTAACAATCAAGGATTTAGTTGAAATTATTGGTAAAGATGCATTACGTTGATTTTTAGGGTCATCATCTATGAATTCGCATGTTATTATTGATGTAGATATTGCCTTATCAAAAAATAACAATAATCCTTTATATTATGTTCAATACGCACATGCTCGTGCCAATCAAGTATTAAATAAACAAGTTTATGAATTAGATTTTAAAACTGATCTATTAACAGAAACACGTGAACGTGAATTGTTGAATCAACTTCATTTCTATAAACAAACAATTGCTAATGCTGCAAATAATCGTGAACCACACCGAATTAGTAATTATTTATATGACTTAGCTCAAATTTTTCATAATTATTATGCAAATGTTAAAATTAATAATGATAATAATAAAGTTCTATCAGCACAACGTTATACATTAGTATGATGTGTTAAACAGGTTTTAGCTAATGGTTTGGCAATTATGAAAATAACTCCATATGATCAGATGTATTAAAAATGAATAGTTTAAATAAAAAAAGTATAAAAGATCTTGATGTAAATGGTAAAACAATAGTTTTACATTTAGATTTAAATGTTGTTGTTGATTATGAAAATAAACGCATATTAAATGATCGTAAATTACGAGCATCACTACCAACTATTAATTATTTAATTAATCATAACGCTAAAATTGTTATTTTAAGTCATTTAGGTCGAATTAAAACCTTAGCAGATAAACAAAGTGGTAAATATAGTTTAGAAATTATTGTTGATGAACTCCGTAATCGTGTATCCAAGAATGTTAATCGTGTTGTATTTTCACCTTTAAATTATGGAGAAACGGTCGTACAAATGGTTAATGATTTAGAAGAAAGAGACATTTTAATTTTAGAAAATACGCGTTATTGTGATATTAGTGATGAAGGTGAATATGTTGGGCTTGAATGAGATGGTTCAGAGATTTTAGGACAGTTTTGGGGAATGCTAGGGGATATTTTTATTGATGATGCATATGGAGTTGCTCATCGACAATTATCATCAAATTATCAAACAGCTAAATTTGCTAAAAAAAGTGCATTAGGTTTTTTAATTGTTAATGAAATTAACCATCTAGATATTGCTTTAGAAGTTCCTAAAAGTCCGTATTTAGCTTTAATAGGTGGTAACCGTGTTGCTGATAAAATTGCAGCAATTGAAGTATTATGTGAACGGGCTGATCAAGTTATTATTGGGGGAGGATTAGTCTATACATTTTTATATGCTCAAGGGTATAACGTTGGTTTAAATTTAGTAGAACGCAATATGATTGATGATTGCAATAATATATTAGAAAAATACGGTAAAAAAATTTTAATTTGTTTTGATTTTTTATGTAACAATGATTTTAGTGATACAAGACCGATTTATCGTAAAATAGATGAAGGTCTTGAAGGGTTATATGGTTTAGATATTGGTAAAAGAAGTTTAAAATTTATTAAACATGAAATTTATCGTTCTAAAACTATTTTACTAAATGGTCCCTTTGGTGTTATTGAAAATATTAAGAATTATGCACAAGGTACTACTGAAATATGTAAATCAATGGCAAAACAAACAACACGTGGCGCTTATACAATTATATGTGATATCGATACTTCGAGTCATGCTGAATACCTAGGATTAGATAAATATATTAATTTTATATCTACTGGTGGGGGCGCTAGCTTATCATATATTGAAGAAGGAGTTCTTGATGGTCTTGAAACCATTGATAATGTTCCTTTAAATGTTTTAAAGAAGGAGTAATTTATGAGTAAATTTAACGAAAAAGAAATTAGAAATAAAGCAATTGAATATATGGAAATATATGGTATGTCACGTCATGAAGAACGTGTTGCTTCCAAACTAAAAGCATCATTAAAAGATGCGGGAGTTGATTATGAACGTGATAATTTAGGTTCAATTATTTTTAAAAAAACAAATTCAAATAAAGGACCTAAAATCTTAATTGCTACACACATGGATGAAGTTGGTTTTGTAGTTCAACAAATTCTTGATAATGGTCAATTACTATTATCAATGGTAGGTGGTGTATGGCCTAATATTGCTATTGGATCAGTAGCAAAAGTGTATGTTGATGAACAACGTCAATATACTGGTGTATTTGGCCATACTTCAATTCATATCTTAGAACCTGAAGCACGCAGTAAAGCAGTTCCAGTTAAAGAATTATTTGTTGATTGCGGTTTTAATTCAAAACAACAAGCTATTGATTTAGGAATTGAAATAGGTACGGAAGTTTATATGGAAGGACCAACTTTAAATTTTTATGATGAAAATTATATTGTTGGTAAAGCTGTTGATAATCGTGTAAGTGTTGCGGTTTTAGATTTATTAATACATAGTTTAAAGGACAAAGTAATTCCCAACCAAACTTATTTTGCTGCAACAGTGCAAGAAGAAGTTGGATTACGTGGCGCAAAAACAGTTGTTTCAAAAGTAAAACCTGATATTGGGATTGTTATTGATACAACAACATCACACGATACATACAAGTGTCCAGAGGGTGATACAAAATTAAATGATGGTGTATGTATTAGAATTAAAGATGGTGGAACTTTAGTTAATCCTAAATTAGTAAAATATTTTGAAGAATTAGCTATTAAACACAACATTCCTTTATATAAATATGTTGCACGAGGAGGCGGGACTGATGCTGAAGAATTGCAATATGGGCCTAATGGTGGAGTATTAACAATTGGTTTGTCTATCCCACAACGTTATTTACATGCTCCAATTGGCGTAGCAACGGTTAAAGATATAAAAGCAGCCTTTGACTTAGTACGTGAGTTTTTAGAAGTATTTGATGAAAACGAATTTGAAAAAGTAAAATTTAAATAATTTTTATATCAAATAAAAACCTTTTAATATAAAATTAAATATCGTAATAAATTTATTTTTAGGAGAATAAAATATGACTGCACATAATCAAGCTAAATTAGGAGAAATAGCTAAAGTAGTACTAATGCCAGGGGACCCAATGCGCGCTAAATGAATTGCTGAAACTTTTTTAGAAAATGCAATTTTAGTTAATGAAGTTCGTGGCATGCTATGCTATACAGGAACATATAAAGGCAAAAAAATATCAATTATGGGCCATGGTATGGGAATTCCTTCGATCGGAATTTATTCATATGAATTATATAAATTTTATGAAGTTGAAACAATTATTCGTGTTGGTTCAACTGGTTCATATAAAAAAGATTTGAATGTTAATGATGTGGTTTTAGTTGAAAAATCATATAGTGATTCTACATTCGCATCACTGATTGGTGCACAAGTTGGCGATGATAAGGTTTTATTACCATCAAGAGAAGTTAATAATTTAATTAAAAATACTGCGAAGGAATTAAATTTAGAATTATCATTAGCAACTTGTCATGCTTCTGATGTGTTTTATAATAATGATTTTCAAACATTAGATGAAATTATTCATCGAACTAAATCCGATTGTGTTGATATGGAATCATTTGGTTTATTCGCAAATGCACAAATTTTAAATAAAAATGCAGCAACTTTACTAACAGTTAGTGATTCATTGATTACAGGTGATGCACTATCACCCGCAGAGCGTGCTAATACTTTTAAAAAAATGGTAACATTAGCCTTAGAAAGCGCAATTAAATTACTTTAATTTTAAAAATAAATAAATTATTATTTAATGGTTAATTTTTATAAAATTAATTATAAAAATAACATTTACTAAATTCAAAGAAAGAACAAAGCCTTATGAATGCAAAAATTAAAGCCAAAACACGTTCACAAATGATAGATGAATTATCAAAAATGTTAAATATTGAAAAAAAACAAACAAAAGCATTTATGGATACTTACGAAGCTTTTTTAATTCTTGAATTATCACGAGCTAAAGAAGTTCGTTTAGGAAATATTGGAAAATTCAAGGTTAGTGTACGTGCAGAACGTAAGGGAATTAACCCTAAAACGGGTGAAACTGTAATTATTCCCGAGAAAACAATTCCTAAATTTACTTTTACTAAAGGAATTAAAGAAATTATTAATGCTGGTATTTCAATTGATAATGAAAGAGTATCGATTGATGATAATGATTTTGATGATGACGATGAATTTGTAGAAGAGTACATTGTATCAGAAAATAATTAATTAATAATATGAAAACACGAGTTTTAATAACTCGTGTTTTGTTATTATTTAAATAAGTATAATAAATGATTATAACTTATTTTTAATAATGGAGTAATTATGAATGAATTAGTTAAAAAAAAATTAGATGATTTATATGATTTTCTTAAACAAAAAAAATATTCAAGCCAAACCATCGAATTAGTCCATAAATCATTTGATTTTGCAAACTATTATCATGGTGATCAAACAAGAAAGTCGGGAGAACCATATATCATTCATCCAATTGCAACTGTACGAATTTTAGCTTCATGAGATATGGATGAAAGTACATTAGTAGCAGGTATGTTACACGATGTTTTAGAAGACACAAATTGTCCAGAAGAAGTTATGGAAAAAACTTTTGGTAAAGAAGTGACGCAACTTGTTTGTTTTGTAACAAAAGTATCTCTTTATTCAAAAAATCGACGTAATAAAGTTTCACACACCAATCTTGAAGAAAAGTATTCAATTCAAGTGTTTATGTCAATGACACAAGACATTCGTGCTATGATTATTAAAATTGCTGATCGTTATCATAATATGCAAACTATTCAATACTTACGTTTAGAAAAAGCGAAAAGAATTGCAACAGAAACACTAGAAATTTACGCAAATATTTCTGGAAGATTAGGGATGTATCGTGTTAAAACAGATTTATTAGATATGTCTTTTAAAATTTTAGACCCACAAAATTATCAATTAGTAAACGATTCAATTAATAAATTGGTTGAAAACAATCAAAAAAAATGAAATGAAATTTTAGCTCGTTTAAAAAATATTTTAATAGCTAATAATATTAAGGCTGAATTTGAATGGCGTCTTAAAGGTATTTACTCAACATATAAAAAAATAACTAGTGGTTATGAAGTTAAAGATATTCATGATATCTATGCTATACGTGTAATTTTAGATGATGTATATTTATGTTATCAAACATTAGGTTTAGTACACATGAATTTTACGTATATTATTAACACTTTTAAAGATTATATTTCAACACCAAAATGAAATTTATACCAATCTATTCATACAACAATTTCGTATGAAGGAACTTTAGTAGAAATTCAAATCAGAACAACAAAAATGAATTTATTTGCTAATAATGGTTTAGCAGCACACTGAAAATATAAAGAACAAAAAGATAATAAAACTGAAATTTTATTAACAGTTAATAATGTTTTATTGCGTGATTTTTTATTGAGCACAGATAAAGGAATTAAAACTATTAAAGATGTCACAACCGGAACTATTTTTGATGTACTAATTCTTAATTCTAATAAATGAATCACTGTTTCAAATGGTTCAACATTATTAGATGTAGCATATAAATACGATATAAATAATTTTTTCAATATTTTAGCAATTTATCGGAATGGTGAGCGTTCACATTTTGACACGCATGTAGAAACTGGCGATACAATTAAAATTAGTTATTCAAATGGAATTGAAACAATTAGACCAAACTGGATTACATTAGCTAATAATGATGTTGTTAAATTACATATTAACAATCATTTAAAAAAATATGAACTGAATAAACAAATTACAAAAGAACAATTTTTAGCTAATGCTGAATTTTTTTTAGAAGGCAAAATTATGAGTGAAGAGCATGCTATTAAAAGACTAGAAAAAGAATTTCAAGTTAATAGTATCAATACTTTTTTAGATAATATTGCTAATACTGACATTGATTTACAAACAAAATATAAAGTATTTTCAGAAAATAAACAAGTTTCACGTGAAGCAATTAATACAATTAAAAATCAAGCATGAAAATGATTAGCTGATTCTTCATATTTTAGTGGTCTAGAGAACTTGTATTTTACAGATTTTGAAATTTCGCCTTGTTGTTCTAAAATTCCTGGAATTAATTGTGTTGCTAAAATTGTTCGCAATAAAGTAGAAATTCACCGCAATGATTGTTCTAAAGTTAAAAATTCTCGCTCAAAAACAATTGTAATTGAATGATCAAAAGAAAAACTTGAGAATCGTCCGCGTTATTTTAAAGTTAACTTAACATTAAAAGGCAATTGAAGTGAATCTGTTGGAAATGTTTTATGCAACACATTAATACGCATGAAAGCAAATATTTCAAAAGTTGATATTAGTAAAAATAAGACTGCACGCACTCATGATACTTTTTTAAAAATTTATGTAAAAAACTTAGATCACTTACAAAAGATTATTTTAGAACTTCAAGCTAAAAATGTTATTAATGAATGGAGATTGATCTAATGAAACATATACAAAAAACAAAATTTATTTTTGTAACTGGTGGTGTTTATTCATCATTAGGTAAAGGGGTTAGTGCTAGTAGTATTGGTCGAATTCTTGTTGAATTAGGATATAGTGTTGCAATGCAAAAGCTTGATCCATATTTAAATATTGATCCCACTTATTTATCACCATTACAACATGGAGAAGTATTTGTTACAAAAGACGGTAAAGAAGCAGATTTAGATTTAGGTACTTATGAACGTTTTATTAATGCTGATTTAAATAAATATGCGTCAGTAACTTCAGGAAAAATTTATTATGAAATTTTAACAAAAGAGCGTGAAAATGGTTTTGACGGTAAAACTGTTCAAACTATTCCACATGTCACATCTGCAGTTATTGATTACATTAAAAAAATTAAAGATAGTTTAAAAACTGATTTTATTATTGTTGAAATTGGAGGAACAATTGGAGATATTGAATCGCTTCCATTTATTGAAGCTATTAGTCAATTTAAAACAATTTATGGTGTAAATAATGTAATGTTTATTCACTGTTCACCGCTTATTTATATTGAAAAAGTAGGTGAATTAAAAACAAAACCAACTCAACATAGCGTTAAGACGTTACGTTCATTAGGAATTAATTTAGATTTATTGTTATTAAGAACTAATCAAAAATTAGATGAAGTAACGATTAAAAAATTAGCATGATCTTGTGGATTAGATATTGATATGATTTTTGCTGCTTATGATGTTGAGTCAGTTTATTTATTACCAAATGTTTTGTTTGAGCAAGGGATTCATAAAACTATTTTAGATTTTTTTAGTCTACCTTTAAAAAATGATAATATTAATTCATGAATAGATTTTACTGATAAAATTACAACCTTTAAAAAACATAATTTAGTTATAGGTTTAGTTGGAAAATATGTTGAATTACCCGATGCTTATAAATCAGTATTAGCGAGTTTAGAATTAGCAGCAATTGAATTAAATATTGATTTAAAGATTAAATATATTCAACCCCAAAATTTAAATGAAAACAACATAAATGAAGAACTAAAAAAAATAAATGGTATTGTTATTCCAAGTATTGCAGGTTCAATAAAAGGCTGACCAGGAGCTTTATTAGCTGCATCTTATGCAAGAAAAAACAATATTCCATTTTTAGCTGTGGGAACTGGCGTTAATATTGGTATTGGTGAATTCATTAATAATGTACTAAAACTTCCAATTGAATTTATTAATTTAGGCAATGGTGATTTTAGTTTTTTAAAAGACGCTTTCGTTAAAAATGAAATTGAAAATTATCGAATTGGAGAGTATTGTTCTAATATTCAAGCTAACACTATAACAAGCCAAATTTATCTCAAACAAAATCAATTGAACGAGCGCCATCGTCATCATTTTGAATTTAACAATCATTATATTAATAATTATTTTTTAAATCAAAATTGAAAAATTGGAGCAATTTCAGTGGATAATAATTATATTGATGTTTTAGAATACACAAAAAATCATTTTTATGTTTTAACAATTTTTAATCCAGAATATACATCAAAACCTTCAAAGGCAAATCCTTATTTTATTAATTTATTAAAGATGAGTTTAAAAATAAAAGAAAGTTAGAATATGATTAATTACAGTAAACCACGAGGTACTATAGATTTATATAATAAAGAAATGAATGATTTTAAATTACTTGAAAATTTTTTATTATCAACTACTAAAAGATATGGCTTTCAGCAAATTAAAACACCTGTTTTTGAATTTGCAGAACTCTTTATGAAATCAGCTGGTGAAAGTTCAGATTTAGTTTCAAAAGAAATGTATTTATTTAAAGATAAATCAGATCGCTGACTAGCGTTAAGACCTGAAGGAACAGCAGGTGTTATTCGTGCTGTTGTTGAGAATAAATTACTATTAAATCATCCACTACCATTAAAATTAATGTATTTTGAGCCTTGTTTTCGTTATGAACGCCCTCAAGCTGGACGTCAACGCCAATTTCATCAATTTGGTGTTGAAGTGTTGGGAACAAAAAATATTTATTATGATTTTGAATTAATTGCTTTAGCTGATACAATTTTAAAAAAATTAATGATATCTAATTATATTTTAGAAATTAATTATATCTCATCTCCTCATAATCGTTCATTATGAGTTAAAAGTTTGCAAGAATATTTTCATTTACATCGTACTGAATTAACTCCATTAAGTCAAGAACGTATTACAACCAATCCTTTGCGTATTTTAGATGATAAATTAGAATCTCAAAAACTAGTTGTAAAACAAGCTCCAAAAATTAGTCATTTTTTATCAAATGAAGAAAAAGAAGAATTTAATCTAATTAAAAAAATGCTTGATGATTATAACATTAAATATTATGTTAACGAAGGATTAGTAAGAGGTTTGGATTATTATAGTGGATTAGTATTTGAATTTATTTCAACTTCACCTAAATTATTAGGTCAATCAACAATTATAGGTGGAGGCCGATATGGTGAATTAATTAAACAAACAGGTGGCCCTAATTATGAAGGTATTGGTTTTGGTATAGGAATTGAACGTTTATTAATAGCTTTATCTGAAACCAATAAAAATATTTTAAACACTGATGATGATAAATATTTAATTGCATTTTTTGATAAAGAATTAGAGAATGAAGCTATAAAACTAACTCAAATATTACGTATCAATAATCAATTAAATGTTGACATTATCTTAAGTTCAACTAAAGCTGACAAAATTTTTAAGTTAGCACAACGTTTAAATGTAAAAAAGCTTATAATATTAGCTAAAAAAGAATGATCAGATAAAAAAATAATTTTAAAAGATTTACTTAATTTTAAACAAGATTTATTAAGTTTAGATGAAATTAAAGGAATAAGGTAATAAAAATGAAAGTTTATTGTGGTTATATTGGTGAAGAACATTTAGAAAAAAATGTTATTTTAAATGGTTGAGTAAAAAAAGTTCGTAAAATGGGTAATTTAGTTTTTGTTGACCTAAAAGATCGATTTGGAATTGTACAAATTTTTGCAACAAAAAGTGATGGTGTTTTTAATGAATTAACACAACTTTCACGTGAGGATGTAATTAATGTTGAAGGTTTAGTTTTGTTGAGAAAAAACCCTAATAATGATTTAAAAACAGGTAGATTTGAAATTCATGTCAAAAAAATACTTATTTATTCAAAAGCAAAAACACCACCATTAATTATTGAAGATGAAACTGATGCAAATGAGGAAATTCGTTTTAGATATCGTTATTTAGATTTACGTCGTGATGTTAATTTAAAAATTTTTGAATTGCGAAGTAAAGTTTACCAAGCTTTTAGAAATTACTTATATTCACAAGACTTTATTGAAACTGAAACTCCTATTTTAGCAAAACCAACGCCAGAAGGTGCACGAGATTTTTATGTGCCAACAAGAACTAGAAAATTTTATGCTTTACCACAATCACCTCAAACTTTTAAACAATTATTAATGGTTGCGGGTTTTCAAAAATATTTTCAAATTACAAAATGTTTTCGTGATGAAGATTTAAGAAGCGATCGTCAACCAGAATTTACTCAAGTTGATATAGAATTATCATTTGCTGATGAAATAGAAATTCAGACATTAATTGAAAATTTATTAAAATATGTTTTTAAACAAACCATTAATGTTGATTTAACAACTCCTTTTATGCGAATGAGTTATGAGCAAGCGATAAATGATTATGGTAGTGATAAACCAGATTTACGTTTTGATTTGAAAATTAAAACACTTAACACATATTTTGAAAATTCAAAAACATTATTTTTTCAAAAAGCACTTTTAAATAATCAAAGTATTCGTGCAATTTTAGTACCTAATATTAATTTAAATAAAAAACAAGTACAAACTCTTGAAAAGTTTGCCAAAGACAAAGGTGCTAAAGGTTTAAGTTGAATAAGTATTAAAGATGAAAAAATTATAGATGGTTCGTTATTATCAATTCAAGAAGATCATATAATTTATAAAACGATTTTTAAAGACTTTAATTTATCAACTGGATCAATTTTATTAGTTGCTGATACTTTTGATATTGCATCTCAAGCATTAGGTTTAGTACGTATAAATTTAGCATCTATTCTTAATTTAAAAAAACCCAATATTTTTAAATTTGTTTGAATTATTGATTGACCATTATATGAATATGATAATGAGGCTCAACGTTTTGTTGCTGCACACCATCCTTTCACAATGCCAACTTTAGAAACATTAAATACTTTTGATGTAAATAAAAAAGACGCTAGAGGTCGTAGTTATGATATTGTTTTAAATGGTTATGAATTAGGTGGAGGATCAGTTCGGATTATAGACCAACAAATTCAACGCCGTATGTTTAAATCAATTAATATGAGTGATGAAGAGGCTAATTTAAAATTCGGTTTTTTACTAACTGCATTCGAATATGGTGTTCCGCCACATTGTGGAATTGCTTTGGGTTTAGATCGTTTGATGATGATTTTAGTGAATAGTGAATATATTCGCGATGTTGTTGCATTTCCTAAGAATAACAACGGCGTTGATATGATGTTAGACGCACCATCTAACATGAATGATGAAGATTTAAAAGAATTAGGTTTAAAAATAAAAAATGATTAATAAAAAGATAAAGATTAACCCAAATATAACATATTTTAAACAAAAAATTTCTAATTATCATGAGCATTTGTTAGTTTGAAATGATTTATATGATAAATTAATTTCATTGACTAATAATATTATTGTTGATAAAAATAACAAAAATATTTATCATCAAAACGACAAACAAATTTTTGATTTTACTTTATGTTTATCTGGTCTTTTGTCTAAAACTTTAGGTTTAAATCATAAATCATCTTCGCTTTATTTAACAAATGATGATGAAGTAGCGCATCGTTTTAAGATTAAAGTTTTGCATAATTATTTTAATTTATATAATATTGAAGAATTTTTTACGTTTATGGATAATCAAATTCCTTTAGAAAATGCAAAAAATATTGCACGTTTAAGTTTAATTTATGATTTAAAACAACATGAAAATATTTTAAAAACTATATCGAATGAAGGTTTAGAAAATATTGATTTAGAATTTGCTTTCATTAATTTTTTAAGAGCAAAAATCTCTATTAATTTTGCCTATAGTTATGATATTTCTGAAGTAATTTTATTGTTGCGAACAGGGCTAGATTTACATTTTTTTAGAAAATGAGCATTTTAGTAATCTTATACCAGTTTTTGGATTAGAAACAATTAAATATTTCAAGAATTGAAAAGAATTTTTAATTAACTATGTTATGACCGTTGCATATCATAATTTAGATATTAATGACCCTAAAAATGTTCTTTTAGCAACGCAAAAATTCTTTAATCATATTAATGAAATAATTATTGATTATGGTTTAGATAAACGTGATCAATGAATTAAAAAAATTATTCCTTTTGAAGAATAATTATAGTAAAATAAGAAAGTCAATAGAGAGAGATAAATTTATGATTAAGAGTATTTGAATTGCTGGAGGTTGTTTTTGAGGTATTCAAAAATATTTTGATAGCATTATTGGTGTTAATCATACAGTTGTTGGTTATAGTCAAGGAAACGTTATTAATCCAAGTTATGAACAAGTTTGCACTCAAACAACCAACCATACAGAAACTGTTCAAATCGATTATGATGATCGTTTTGTTAGTTTAATAAGTATTTTAGAACATCTTTATCAAATCATTGATCCTTTTAGTTTAAATAAGCAAGGCGATGATGTTGGTAGTCAATACCGTAGTGGTATTTATTATGTGGATCACGATGATGAGTTTATTATTAAAGATTTTTTATTAAAAAAACAAAATCAAACACCAAAAAAAATTATGATTGAAGTTGAAAGATTACGCAATTTTAATATTGCTGAAGAATACCATCAAAAATATTTAGATAAAAATCCTAATAGTTATTGTCATGTTGATTTATCATTATCTAAAAAAGAATTCAGATAAAAACCAGGCGTTTTTGCGTCTGGTTTTTATATTTTTTAATCATTAATATTATTACAAGATTGATATTTCTCTAATTTTTTAATAACATTATCTTTTAAAACGTTAGTTAAAAATACCGCCATATCATGAATTGATTCTTTAGCACCATTTTTTAACCATTGCATTCTAATATTACAAAAAAATCCTGTTAAATAATTAATTAAATATTTATCAATTGAATTATTTGACATATTACCTATAGCATAAAAACCACTTTCAGTGGTTAAATCAACAATTAATCCACTAAAATTATTTTTATCTAAGATTAGAAAATATTCTTTGAATTCTAAACTAATTTGTAATGAAATTTCATAAAATTTAGTAAAACCAACTTCATCAAAATTGATATTTAGGTTATTATTTATTAATTTACTTACAACTTTCCAATTATAAATATTTTTAATAAAATAATTAATAATCGAAATTTTATCACTAAAATTACGATAATATGAAGGTCGCGAAACATCTGCAACCTCAATAATTTGTGAAATTTTAATTTTGTTAAATGAATTGGTTTTCAACAAATTAAATAAAGCTGAAACAATTTTTAATTTAACTTTTGAAATTTTAATTTTTTCTACTGCCATTTATTTTTATAAATTATTTTTAATAAATTCTTCTAAATCATTTGGTTCACATTTTGATTCAAAACGTCCAATAATTTCATCATTTTTAACAAAAAATTTCTCAAAATTTCATTTTACTGCTTTTGCTTTTGGACTTCATGGATACTCAGTAATTAATTGTTTGTATAATGGAGAAATATTAGAACCTACAACATTAGTTAGATCCATTATTGGAAAAGTAACATTGTATTTTGTTGAACAAAATTCCTTTATTTTATTATTGTCATCAAATTCTTGAAACATAAATTGACGGCAAGGAAAAGCGACAATGATGAATCCTTTATTCTTATATTTTTTGTATAAATATTCTAATTGTTCATATTGTTTAGCGTATCCACATTTACTTGCTACATTAACAATTAAGACTAGTTTATTCTTAACTTTTGATCAATTAAACAATTTTTTATCGATATCTAAGACCGGATAATCACTTAATTTCATATTTAGCCTTTCCATATTTTAATGTAAAACATTGAAATTATACTCTAAATTTAACAATATTATATACTAGTTAGTTTTTGTAATATTTATTATAAAGTATAAGATGGAATCTAAGTATCTATTAAAACATTAGTTGCTTTTGATATTTAATAAAAAATATTAAAAAAAGTTATGATATTATTTTTGCTAAAATATATTAGGTAATTATTAAAAATAAAAAGATGTTTAAAGATTTTTTGTAATTTATTTACTAGATCTTTGTCTATCTTATTTTTACTATATTATTGTTTTTATAAATGTCCATTTTAAAACAAATTACGTTAGGAAGTGTTCAATATGGATAAAAAAAGTAAGAAAATTCTAGGATTAAGTGTTGCTTCAATTATTGCAGCACTTGCGATTATTGCTCCATCTACTTATTTTGGTTTACAAAAACAAGAAGAGGGTAAAAAAGATATTAATTTAGATAATGTTACGCGAAAAATACCTGGTGAAACTAAAGATGATATCAAAGTTGAAGATTTAAATAATATTAATGATCAAAATACAGATCTTAAACTTGATTTTAGTAAGGTAAGTATTAAACCCCAAACAAAAATTAATTATTTAGCGATTGGTGATTCAATCACAGCTGGTTTTAATTCAGAATTAGGTTGAGAAGCCCCAGGTCGCTATGATCCAATAACTAATAAAATTAGTGGATTAAGTTTTCCTTCATTTATTGCGCAATACATAAATAAAGTTGAACCAAATCGTTTAGCTTCTTATGAAAATTTAGGAATCACAGGATCACGCATCGAAGACTGATTATATATGTTAGGTGCTGGAAGTGATGAGTATTTTAAAAATCAAGAATATAGATTTTTTGATTTTTCTAAACAAATGGATCAACAAAAAGATAATCCTTTTAAAAACCGTTTATCAAAGTTTTTTAAAAATTTTGGTTATGATTCAAAATTAACACCAAAAAATAATCATGAAGTTAATATTAATGATTTTAATGGTTTAAAAGAGCAAATTAAAAAAGCTAATTTAATGACAATCACCTTAGGTGCTAATGATTTTTTAAGATATTTAGACATTCCAAAACTTGTTAGTTTAACAAATTTGAAAGGTAATGATTTAGAAATAAAAGTTAAAGAGTTCGAAAATAATTTACAAAAGGCATCTTTAGAAATTACAAAAAATCTAAAGAAATTAATCGCTCTTATTCGTACTTTAAATTCTAATGTATCTATTACATTAGTAAGTTATCCATTACCACTATTACGTTTACAAGATGTAATAGATAGTTCATTTAAATTAACAAATAAACGTCGTTTAAGTGATGAAATTATAAATATTCTAAATAGTGCCATTAAAAAAACATATGATTTAAATAATAATATCAACTATATCGATGCTTTTGATGAGAATGATTGAAAAGAAAACAGTTTTAATTTTGCAAAAGCAATTTTTGATATTCATCCAACTGAATTAGGATATAAACGGATGGCTCAAGATATTTTTCTAAAAATGTCTTTGGGACAAAATTACGCTCAGCAAAAAACTGCTTCTATTGCAAATGCTATTTATGATGCATGAGATAGTGAATATTTTAATAAAGATAGCGATAGTTTTAAACAACAAATTGGTTTTGCTAATAACACAAATCAAGATCTAGTAGAAAAAACTTTAGGTTCTTCAAAAGGTGCGGCATTTTGAGCGAAAACTGCTCTTGAAAATGATGAAGAATTACGTAAATCCTTTTCTAATTTTAAAAATACCTCAACAATTGTTAAAGATTGATTAATTTCAAATAAACGACGTTTTGGGAGTTTTGTTAATTTAATTACTTCAAATTTAACATCATTAGGATTAGATAAAAATGGATATATCAGTCAATTTTTATCAAAAAAAGATGCTGATAACACTAATAATTTATATAAATTAGCATCAGCTTTAGCATCAACACCATATATTGATATGGTTATTTCTGGAATTCAAAATGATTTAGATAATTTAGATTTAGATAAAAATAATATAATTGGGACACAAAATATTTCTAAAAAAATTTTATATGATGTTTTTAGAAAAAATATTATCAATGATCAGAATTTATATGAATTAATTAAAGGAATGTTTCATTCTCAGCTATTCAATGAAAATAAATCTCAAATTAAATCTTTAATAAAAGCTTTATTAAGTGATTTGGTTAGTTCCCATAAATTATTAGAAATAATTTTTGGTGAATTTGCAACTGATAATTTAAAGAATAATAATGAATTCTTAAAAAATTTACTAATAATCCAAAATAAATTAACTCAAACAAAATCACTAGATAACATTATTAATGTTTTTGTAGATGCTTTGTTTGATAATGATAATGAATATTTATTGCAGGGTCCTTTATCAAATGTTTTAGCAAAAATTGTTAAGACTAATAAAGCATTGTTAACAACTAGAATTGACAATTTATTTAATGATTTATTTAAAGATCAAACAACATTAGATGCAACTTATAACATTCTAATAAGAGTTTTAGGCCAAAATATTCCTGATTTTATAAAAACTCCGCAAGCATCTTTAATAATTAAAAAAATTTTATCAAAAGGTACAAATTTAAAATTAATAAACACTTTAAAATTAAATACGATTGATTTATTAACTGATTCAAAAACATATGCTGGCATATTTGATTCATCAGAAACTTCAATTATCAATCTTATTCAAAAATCCATCGATTTTACTTCAAAATCATTTATTAGCGATGTTTTAGATTTATTAACATCAGATAATGTTGATTTAAACGAATGAAAAGAATTTATTAAAACAATTTTAAACTCTTCAACAATTAAAAATATATTTAATTATGGTATTGTTAATTTAGTTTCTTCTAATACCACACCATTAAAAACCAGTCATTTTGATTTTAATGTTTTATTAAAAAATTTAATTGCTGTTTTAGAGGAAAAAGATTGAACAACCAACCAAAAAACAAAATTAAAAAGCCTTTTAAGAATGAGTGTTGATGAATTATTAAAAAATCCACAAACTCAAAATTTTGTTACTTCTATAGAAGATTTTATTGCTAATAAATTAGGCGATCTGGTAATTAGTGCAAATAATAGTTTACTAAAATCAAATATGTCTGATATTTGAAAACTAATCAAAGAAGTTGTTCATGACTTAATTCATAGTTCTGAATCATATGATCTAATATTTTCAATTATTAATGACTTTATTGATCATCCACAAAATTATGATAATTTAAATAATATTAATGATTTATTACCATTAATTTTAAAATCTACAAATAAAGAACTTCAAGATAAGGTTGATATATTATTTAAAAAACTTATTAACAATGAAAATATACCAAATTTAGTAGGAACATTAACAACAAATTTAATTGTCAAAAACTTCTTTGTTAAAGAATTAACAGTAGATCAAAAACAAAAGATTAATAATTTTATCACTAATATTTTGCCAACAATACCTAATTTAACTTTATATAAAGAAATCCGAAAACAAGCTTTTGAATTTTTAACAAACAATATTAATAAAATTTTAGTGGATCCTAAAAACACTTTATTAATATTAAATGATGGGATTAACAAATTATTATCAAACATATTGCCACAATTATCAACCCTGATTGAATTAATTGATAACGATAAAATTAAAGATCAAGATTTTATTGATGTTATTAAAATATTTATAGACAATTTAGATTTTAATAAATTATTTGTCAATATAAAGTCTAATAATAACCAAACAACAGATAGTACTACTTTAAATCAATTTTATTTTAAATTTAAATTAAATTTAGAACCTAAAAAATTAATTTTCGATTTAATAAAAAATCTTTTGAATTCACCAATTTTAAAAGTAAATGATAATAAAAATAATAAAATTAAGATTGAATCTAATAAAGCTAAAATTAAGTCTATTCTAACAACTAGTGTTACAAACATTTTTGAAAATTCTAATCTTATGAATTTTTTTAATAATCAATTATCACAATTATTTAGTTCAATTGATTTATTTAAAAATTTAAATTTATCAAATCAACAACGTTTAAACTTTGCTAAGGCTATTGTTGAATTATTAAATAAAGAAAAATTATTATCAAATTTAATTAATGTTTTAATAACTAACTTAATTGATCACGCTGATGAATATGCTGAAGCAACAGATTTAGCAACAATTTTATCATTAACAATAAAATATAATCAAAATGAATTAAAAAATGCGTTTGATAAATTATTAATAGGAACTTTAAAAAATGATAATCTTCAAGATTTAATTTTACGTATTTTTGTTAAATTAATTAATCCTAACAAGTCTTATGATTCAATTAGTGTAGTTTCAATTAATAAATTGAAAACATTTATATCAAAATTTGCTACAGGAATTAGTAAATTGGATTTATATCAACAATTAAAAAATTCAATATTTACAGTTTTTTCTAACAAAACAAAAATGCAACAATTATTATCAAATGATGTGAGTGTAATAAATAAAACACTACTAGAAGCATTTAACATTGACACACCAGAAAAAGTTTTGAAAATTAGTAGTTTATTAGATATTAATGAAATTGATGCTCGTGATTATGCTGATATTATTGAAATTATTTTAGTTGAAATTGGTTTTAATGTTACAAGTATTCAAAATAATGATGTTAATAATAATTTGAAAACAATTGATAAAAAAACAAATGATTTTGCTTTTCAAATTTTGAAAAAAGCTTTAAATAATGGTGTTAGTGATCAACAATTACAAAAAATTAAAAATGTTGTTAATTATTTATTAGATGATATTGTTAAAATTCATGATTCAAGTAGTTTTTTAAGAATTCAATTAGAACAATTATCACATGTTTTAGTTGATAAAATTACCGCAGTTTTACCTAAATCATTAACAATTAAACATAAATATACAAAGATTTTTTCATCGATTTTAAATAATCAAGATTTTTTACAAAAAGCCAAAACTTTATTATCAACAATTTTAAATGAACTGATTGATCATAAAGATAAATATAAAGACATTAATTCGTTTAGTGAATTAATCTCCGTATTCTTTAAAAATAAAGCTAGCGATCTAAAAACACAATTAAAAGATTTACTAAATACAATTTTAAAAAACCAAACACTAATCACTAATATTGGACAAGTAATAATAGAATCATTTAAACTAGAAAACAAAATAAGTATTCTAGATAGTGATCTTGAACAAAATACGATTTCATTTATTAATAAAATTTTTGCTCACATTACAGAATTGCCAATTTATACTAATTTAGTGGATAATTTTTTTAATTTTTTTAGTGAATATACAAAAAGTTCTGACACAAAAACATTGAATTTTAATAAATTTAAAAGTAGTTTATTCCAAGCTATCATACCTAAAATAAGTGATTTTGCATCTCTTTTAGACTTATTTAAATATAATGAAATTAGCGAACAAGAATGAAAAAGCGCAATTACATTCTTTATTGATTATTTGCCAATAAATAATTTAATAAACTCTAAAATTTTGCCTACTAATTCTTTATTATTTGTTAGAAATATTTCAATTTCAAAAACAAAACCAGAATTAAATAAAATTGAAAACATCATTTTAGATTTATTTAAAGCATTATCACAAAAAGCTAAAACATTTGATAATAATACCTTAACTAAAGCAAAAAACATTATCGAATTTACTTTTAATAAGCTTTTAACATCAAATAATATTAAAAATTTTATTAATAATCTATTATCAAATTTAAGTCCCATTAGATCTTTTTTGAAATCAATTAATGTTCAAGATAAAAGCAAACACAACTTATTGAACATGTAATTAATTCTCTTTTAAAAGATAAACAATTACAAACAATTTTTAACGCCTTAATTGAACCTATTTTATCAATAACAATAATTTTGATAATGTTAAAACTTTATCAGAATTTATTAGTCAGATTATTATTAAAAGTGGAGAGAAAATTAAGCCAGCTATTACTAAAATTATTAAAAATTTATTATTAGATGATGAAATACAAACATTATTATTACGTTATATTATTCCACAAATTAATACAACAAAAACATACGATAGTATTAATAATTCAGCTATTAATAAAATCAAAATTTTAATTACTAAGTTAGCAAATAATTTAGATAAGTTTAGTATGTATAATGATCTTTTAGATAGTTTATTTACAAAATTAAGCGATAAATCTATTTTAAATAAGTTATTAAATGATGATTTTTTATCAATTGGAAGTATTTTAAAAGAAATTTTTAATATAAATCAACCATCACAACTAATAAAAGTTATTGATATTTTTAGTATCAAAGACATTACTGCCCAAGATTATGTAAATGTTTTGAATGTTATCTTTAATGATATTGGTTTTAATAATCAACAAACAAAACAAAATATTACCAATGATCAAAAATACGATATTAATATTTACTTTGAATATTTAAAAGCTTTAGTTGAACATAATTTTAGTGAGTTAACTAAAACAAAATTAAAAGAAATCGTCAAATTATTCGTTAAAGATGTAATGAATGATGACCATCATAACCATTTATGAAAAAAAATGACATCGTTTATTAGTCATAAATTAAGTGATTTATTAATTAAAAAAATTCAAGTATTAACTCCACAGAAAAATGAATATCAACAATTAGTTAACAATTTATTAAATGGTATTGATTTTAAAAATGTTGTCCAAAATTTAATTGTTGAAGGCTTTGCAAAAATAATTGATCATCAACAACAATTAAAGAACTCACAAAATTTTGGAGATTTAATTCAAAAAGTTATCAATATAGATGATGTATGAATTAAAAAACAATTAAAACAACTCCTAAGTGCAATTACAAAAGACATAAATTTAACAAAAGCTACATCAACTACTATTATTAATTCGTATTTAAATTATTTTGAAATTAAAAATCTAACAGAAGAAGATAAAAGAAATTTAATTAATATCTTTGACAAAATTTTAAAGCAAATTCCTAATTTATTATATTTACAAAATATTGTTGATCAATCAGTAGATTTTTTAAAAGTAAATATTGAAGCATTAATTAATCATCAACCACTTAAAAACACAACATGAAACGATTTTATTAACAAAAATGTTACAGATATTAGTGCTTTAAGTAACTTATTAGAAATTTTTGAAACTAATGAAATTACAAATAATGAATGAAACCAATTAATTACGATTTTAATTAATCATGCACCTATTGATAAAATTGAAGAAATCATTAGAAACATAACATCAAATTCAATAATTAACAATAAAGCACAAAAACAAGATATTAAAAAACAAATTGATAAAATTTATCTACTTATTAAAAAAGTTTTACAAACACAAACCTTAAATAAAAGCCATGCCAAGACTAGTGTTGTAAAAATTAACGAAACAATTAAAACATTAATTGATTCTTTATTTGCTAATCAAGAAATTAAAACTTTAATTAGTAATACATTATCAAATTGGATTTTCAGTTCGAATTTAGCAAAAATTTTAAATGTTGACGAAGATCAAAAAAATAGTTTAATTAAACCAGTAGTTGAATTTATTATTAGTAGTAATGATTTAAAAACAATTTTAAAAACCATAACAAATAGCTTTATTTTACATGCTAGTGAATTAGCGAACACAAATTCTTTTGAAGAATTAATTGTTAAACTTATTAGTTTTGAGCAAACTAATTTAAAAAGTAATTTAAATAATTTTATTCAAAAAATACTTCAAAACGATAAAATTGATGAATTGTTAGCGCGTGTAATATTTAAACAAATTTCTCCACAAAAAAAATATGATCAAATTCAGCAAATAAATAAAGACAAAGTGGTCACTTTTATTAAGAAGCTTGGTGACAATCTGACAAAATTTGATTTATATAATAAATTACTCGATAATTTATTTAGTTCAATTACAAATCAAACAAATTTAACAAAATTATTGAGTGAACAGCAAGATAGTGTAATTAATTTTATTAAAACGATTTTTGATTTTAGTGATCCTAAACAAATTATTTCACTAATTGATGTATTAAAAGTCAACGAGATTACAAGTAATGATTATATTGAAGTTATTAAATCAATTTTAGATGAAATTGATTTTAATCATTTCTTTAATCAAGCTGTCAAAAACAGTAATCTTTCAAATTATCCATTGAATAAACATGAAAATGATAATGATAGTAAATTAGATATAAATAAGTACTATATTTATGTAAAAACATTATTTGCGCATGAAATGGATTCTACATCAAAAGAAAAATTAAAAACAATTATTGATGAACTAACAAAAAAAATTGTTGAAAATAATAATAAGGGCTTGTTGTACAATTTGGGCAATGGATTAGGTGAAAAACTAAGTAATTTAATTATTAATACATTCCCTGGGTTAACTAATTTAAAACAAAACTATAAAACACTAGTACAAAAAGTATTTACTAATCAAAACTTGTTTGAGCAAGTTCAAAATTTGATCACTTATGCAATCAATAGTTTGATTGATCATAAAAATGAATATCAAAATTGTAAAACTTTTGGTGAATTATTAACAACTTACATTAATAAGAATCAAACACAAATTATTGATAAATTAAAAGAAGCAATTAATAAAATAATCAGTGGTTCATCTTCATTAATTGATGAAATTTCAACATCATTTGCTAATACAATTAAAACATATTTTAAATTAGAAAATTTAACAGTAGAAGATATCAATAAACTAAAGTCATTTATTAATTTTGTTATCAAAAATATTTTCAATCTTGAATATGTTAATGATGGTTTAGTTAAATTAATTACGACTTTAAGTTCAAACGCCATAAAAATTGTTGATCAAAACACTAATTTTGTAACTGTTATGCAAAATGATGTTATTTCATATTTAACATCTCCGCAACAAATTGCAAAATTACTTCAAATTGTTGATTTGGATAAAAATGATGACATTTTAAATTTCTTAAAAGTTATTTTATCTCATTTGCCTCAAAACGCATATACTATTTTATTTGGTAATAAAATTAATCTTATAACAAAGAACACAAATCAAAAAACTAATAGAAGTGTAATTCAAAAATATACAATATTAGATTATGTAAAAACTATTTTAAATTCTTCTTACTTAAAAAAACAAGATACAATTGTAAAAATTAAACATATTTTTAAAGATCTTTTAGTAACTATATCTAAATCATCACAATTAAATAATTATATTGGTGAGTTGATTAGTAATACTTTCGCACAAAAACTTTCAAATCTTAGTGGTGTGGATAAACAATATAGTGAATCATTTTTAAAAGAATCATATAAATTTATTATAAGTGATGAAAAATTAATCAAGATTATTGATGAAGTAGTTAATGATTTAGTTGATCATAAAGATGAATATTTAAAAGAAATTACAAACAATAATCAACCATCAATTAATATTATTTTAAATATGTTAATTAAACGAAATGTTGATCAACAAAAAGTTGATTTTGTACCTTTTATAAAACGTTATTTAGTTATTGATTCTTCAACACAATTTATGGCTCAATACCTTTTTAAAACTTTAAAATTAGAAAATACTAATGATCAAGATGTAAAAATTGTTCAAACATTTGTAAAAGAATTAATTAATCAAATTGATCAATTAGATTTTGTAAATTTATTCATTGATAAAATATTTACTTTAATAAAAGAAAATGGACTTGATTTATTTACAAAAGAAGAGGGTATAAAAAAAGTAAATGAAGCTATGAAAACGTTTGGTTTAACTGAAACAACTAATTTATTAAAAATTGCAAAACTTGTCGAACCAAATAAAATTAAACCCCAAACCATAGGTGATCTAATTAATTTAGTATTTGAAAAATCACCATTAGCTGAAGATTTCGAAAGTATAACACAAGATAAAAATCCTTTATATTATGGTTTAAAAAATCTTAAAGAAGATAGTTTAAATGCTGTTTTATTTGCTAGTGGCAAAAAGAAAATAGGAGAAGCAGCAAAAGTTAGTGAAATTAATATGTTAGATGCTATCCAACATTTAATTAATAAATTATGATCAGCTCGTGCAGAATACGCTAAAAATCATCCTAATTTAGAATATGAACAAGATAATCCATATATGCGTTCGCTAATTCATTTAGGAATTGTGATGCAATGATATGTTCATGAAACATATTTTAGAAATGTTTCAGGTGGGCTATGATGGACTGGTTCTGATTCATTATCGGGTGAAGGACGTGTCTATTTATTGCTAAAAGCAGATATGGGATCTGAAAGTGAAAAACGAGTACGCTCAATGATTATGGGGAATCGTGGTGCAATGCTTTGAAATACACCAAAAGAGTGGAATTATAATAAAAATGATTTTTTATATATGATTACATATTGAAAATGAAGAACAAATTCACGTTTTAACGATTCTAAAGAGAAAAATAAAGCAACTTATATTTTCAAAGCTTTAAAACGCGGTTATGGTGAAAAAGTTAAAACAATGAAATAATTATAAAAATATTAGAGATTTTAAACAATCTTTAATATTTTTTTATTTTATAAAAATAATTTTGATGATATTGTATCTATTAATTTATTTTGTTAAATGTTAAAATATAATAATATTAGTCAATAAAAATAAAAAATGATTTAAGTAAATATAATTCATGCAAATTAATATTAAAAAAATAATAATAAAAATAAGCACTTTTTTAGGGTTAGGAATAATTCCTATTTTAGCAAGTTCTTGTTCTATTATTATTCCTGATTCGATTCCTTTAAAATCAAATGTTTCTTCAATTACTAATGATATTTCAAAATTAGCTAATGATTATATTGATAATCACACAAAATTATTGAGTCTATTAATAAAAATAAAAAAATTAGTTTAACGAATGAAATGATTCAAAAAGCAAAATTTTATGCTGGATATATTTCTCGTCTGCGTTTTATTCAAAAATTAAGATATGAAATTCAAAACGAAAAAAAAACATTGAATAAAATAATTAAAGTTGTGAATGATGCTGATATTAGTACCTTATATGATTTTAAAGATAAAAATCTAACTATTTATACGAAAACATTTTATTTCCATAATTATCAATTTGTCAATGCTAATAAAAAAAATTATTTTATTTCTAAAAAAACTATTAAATCTGTTATTACAGAAAATTTATTAGATATAGGATTTACTTGAACAATTATTTATAATGATCAAACAAAAACTTATATCAGGCCAACTGATTTTATGACCAAAAATTTTATTGATTATAAAAAAAATGAAAATAAAATTGCTACTCGTTCGAAAATTTTATATGATATGATTGTTAAAAATGAATCATTAAATATTAATCATAGTGAATATGAATCTTTATTAGTCAATAATGAATGAAAAAGATATTATGAAAATATGATTAATATATTTTTATATCAATATTATAATGAAGGTATAAAAAACGATATTGATTTTTTAAATCATTTTTTATTAAAAGATGCACTAAAATAGGAGAAAGATATGCCAAGAAAACATTTAATCGCTAGTGGAATTAATAAGAAACAACAACAACAAGCTAAAATTTGAATGAAATGTGCAAAAGAAATTAAAGCTGCCGCAAAAATGGGTGGTCCTAACCCAGAGGCAAATTCGCGTTTAAAAGTTGCTATTGAACGCGCTTTAAATAATAATTTGAGTCGTGATTCAATTGAACGAAACATTAATGGTGCCAGTAAAGATATTGATAATTTAAAAGAATTAACATACGAAGGATATGGACCAAATGGATTAGCAATTATTGTTCGTGCTTTAACTGATAATGAGCAGCGGACAATATCAGCTATTCGTGGTTATTTTTCGAAATTACAAGGTCAGATTGCTAAACCTAATTCTGTTAGTATGTTGTTTAATGAATGTGGACAGTTATTAATTAATAAAGAAACTAAATCTTTAGATGAATGATTTGAAATTCTTATTGATCAAAGTATTATAGATATAAATGAAGATGATAAAATTATTGAAATATTAGTTAAGCCAGAAGATTTTAGCACAGTAAAATTAATTCTTGAAAAAAATAATGCTGATATAAAAAGTGCGGAAATCAAACTTATTCCTAATGATTTCATTAGTTTAGACGAATATGCTCGCGAACGTTTAGTACGTTTTGTAAATGCTTGTGAAAACGATGATGATATTTCATGAGTAATTACGAATTACGAAGAAGAATTATAATGTCAAATATTGATGTAAAAACTAATTTTTGAAAATTAAATCAAAAGAATAAAAAGATTTTATTTCGTGTGCTTGGATATTCTAGTTTATCAGTTACTATAATAACACCTTTAATTTATAAGATATCATATAATAAACAATACTCATTTATTACTAAAAGTATACGAGAATTAAAAACAATTGATACTAATTTTGTTTCATCTTATTCAGATTTTTATATTCTTCAGAAATCAAGTATATCTAATGACTTGTATAATTATAATTTGATTAACAATTATCATGATTTTGAAAAATTAATAAAAAACGATTTGAATTTTGATAAATATAATAGTGCTTTGATTTATGAAAAAATAAAGGTTAGTTTATTAGAGAAATATAATGAAAATTTTTTCAAAAATAACGATCTGTTATATGTTTCTTATAATTCATTAGACTCAAAATATTTTTTAACTTCAATTTTGAGTTTTGATTACAATAATTTATATTTACCATTTTTAAACACTAAATCGATTTTTAATGATGAGTATGATAATAAAAATTTTTATACAGATTCAGCTCTAAGTACTACTAAAACAGAAGTTAAATTTTTTAGTATTAATAAAAAATATAACATAAAACTTAAACAAATTAAAATTTATAGTATTGAAACGGCAAAGGATTTATTTCTATACAAAAACTATGTTCGAAAGAATAATAAACCTATTTAATCATCATAATATTTTAATTTCAAATCATTAATCATTTTTTTAAAATTAATAACACCTGGAAAAATTATTTCTAATTCAATACATTGATCCTTAAATCATTGAAAGGGAATGGATTTCCGCTTAGTTTTTTGATAATAGTTAATTAATTGTTGAAAAGTGATAATAAAATATAGATTGTAATTAATGAAACAAATAATTAAAAAAGAGAAACCTGAATTTTTATGAATTAAATACAAATGTGCTAGTTGGTGCGGTTGTATTTGTTTTATTAAAAATGAGTTTTTATTTGTTTGTTTAGCTTCAAAATCAAAATAATCACCTTTATAGAATCCGTAATAATCAGTTTCACTTTTTTGCTTGATTTTTCCTTGAACATAAGAATTATCAATATTTTTAATAATAATATCAACACACCTTTTTTTAAAAAAAGCTTGATATTGTAAATTAAACAAGCTAATTGATTTATTAATTAATACTTCAAGATGCATTCCATTGTTTTTATTATTCATCTCTAACTCCTATATTAATATAATTAGGGAAAAATAATTATTTTGTATGAATATAAAACATAATAACCTTTTAAATACATAATAATGCTAATAATTAGGTTATAATTTTATATTATTTTTGTAGAAATATGAGGTAATAATAATGGCAACAATTATTCAAGCAAAAGATTTACGTGCTGGCCACACTTTTTTATATAAAGGTAATATTTACCAAGTAATAGAAAATTCGTTTAATAAAACAGCTATGCGTGAAGGAATTGTAAAATGTAAAGTTAAAAATTTACGTACTGGTGCAATCACTATTGAAGTTTTAACTGGAGAAAAACTTGAACAAGCAGTGATTGAAAAATCAAAAATGACTTTTAGTTATGATGATGGTAGTGGTTATGTGTTTATGGATAATGATACATATGAACAAATTTCAATTCCTTACAGTCAATTAAGTTGAGAGAAAAATTTTATTGAGGAAGGTACTGAAGTAAGTGTTATGCGTTATGATGGTGAATTAATGGGTGTAAGTTTACCAGATCAATTAGTCGTAACAATTATTGAAGCAGAAGAAGCGGTTCAGGGTAATTCAGTTCAAAATGCTACAAAACGTGCATGATTAGAATCAAAATGAGAGTTTCAAGTTCCACAATTTATTAAATCTGGTGAAAAAGTGATTATTAATCCATCAAATGGTCAATATGTAGGAAGAGCTAAATAATAATGCAGTACATTACCATTAATAATACAAAATCAGGGAAGATTTTAATCTCAAAATCAAATATTAAAAAGTTTATTGAACAAAAATTTAATTTGATTGTTAATAAAAAATTTGTAATTAAGAATATCGATATTGTTCAGTATGATGAATCCCTTGTTGATATATCTATTATTATTTCATTATTTGATATTCATCAAAAAGTTGATCTTGATGAAGTAAGAGATGTACAAAATCATTTAGCTTCTTTTATTTATTCAAATTTAGGCGTTGATACTAAAAGTGTAAATATAGGGATTGATTTATAAAAATGAAAACTCAATGAGCTAAACGTGTTAAATTATTTCAATTTATCTATCATTGATTAATTACAAAAAAAAATAAACCGATAGCATTAAAATGTGCTTTAGTTGACTTTGATTTAGATCTTAATTGAATAAATGTTGGTGAATATATTTTGGATAATTATGAACAATTAACAAAAATGATTAAACCTCTTATTAGTAAAGATTGAACATTTGAACGTTTATCATATGTTGAGCAAGCTTTATTGCTAAGCGCATATGGTGAATATCTGGTTTTAAAAACTCCTAAGAAAATTATTATTGATCAAACTTTAATTACAACACATAATTATTCTAACAACGAATCATATAAGTTTATTAATGCAATTTTAGATCAATTATTAAATTAAAAATTATGCAACGTTACTTTGTTTCTAAAATTAAAGACAAAAAAGTTTATTTTTATGATTCTGATGTTCATCATATCAAGAATGTTATGCGTATGAGATTACATGATAAAGTTATTGTTATTAATAAAACAAAAGCATTTGTTGCTGAGATTATTAATCTTGAACCATTACATGCAACAATTATTAAACCATATACTCTTAATTGTGAATTAGCTACTAAAATTGATTTATTTCAAGCAAGCATTAAACCAAATAATTTTGAGTGAATTGTTCAAAAAGCATGTGAATTAGGTGTTAATAGTATTTATCAAACAATTTTTAAAAGAACTTATAATCATTCACTAATTAAAACGAATCGTATTTTAACAATTATTAAAGAAGCGTGCGAACAAGCACGACGTAATCATCTTGTTAATTATTACGCTCAATTTAGTTTTAATGAATTATTAAAATATTTAAGAGATTATGATCTAATTTTAATAGCATATGAAAATGAAAAACAAAATCTTTTAAATAATGCTTTTAAAGAAGAATTGACCTATCAAAAAATTGCTTTAGTGATTGGACCAGAAGGTGGTTTTGAACAAATGGAAATTAATTTATTAAAACAATTCGATAATGCTATTTGTATTAGTTTGACAAAAACTATTTTAAGATCAGAAACTGCAAGTCTTTATTTACTTGCAAATCTTGTTAATAAGCTTATTTAGTTTTATTTAAGAAAGGAAAAAAAAGTATGAAGCAACGATTAATAAATTATATTATTGTTTTAATTGGTATTTTATGTATTGTTAGTCTTGTCGTTGGTTTTGCCGTTTGATTTAGCCCTAATAAAAATTTTGATACGTTAACTCAAAACAAAATTGATGGTAATGAAGTTAAAAGATTATATAACCCAAACAATGGCATTTTTTTAGAACAATTAGAAGGGTTTAGTAAATATAATATTGCTGGTTATGTGGTTCCAATTGTTTTTGTTATTTTATCATTTATTTTATTATTTTTATATTATTTTTTAAATAAAAAATCACGATCTAAAGCTGATTTTCCTATTAGTGAAAAATTTATTATCACACTTCAATTACCACTCTTTATTTAGTAGGATCCTTAATTATTATTAGTTGAATTCTTAGTTACATTGCTATTGGTATCAACTTTCGTCCTAATAATTTTAATGTTACGTTGCAAGAAGGAGATTTTTATGCGACAGATAGAGACCCTTTTTTAACATATTGAAAAGAATTAAAACCAAGTTTTTATGCAAAAAAAAGTGTTTATATTGCATTTTTAACTATTATAATTGCTTTTGCTGTTAGCATCATTGGTTTTGTAAATTATTTTTCATTAAAAATTGCCTTAGAACAAACACAAAAAGCCACTTTAGAAGTTCAAAAACAAAAAGAAGAACAAAAAAATCAATTATCAAATGGTGTATATTTTGATCAGGATTTAAATTTATTAAATGAGTATGATAAATTAGTTTTATATCAAAAAAATAATTTATCAAGTATAGTCTTTTTAGCGATTAATGACCAAGAACAAATTTTGATGCAAAAAGATCAGGTTGTAGTTAATTCTCTAAATATTGAACAAAAAGTTAAATATCGTGAACAAGCAACACGAATTGTTTTTGAGAAACAAGCTGAACATAATGATCATCTAGTAACTTCTGGAAATTTCTAAATTAATTTAATCTTAATAAAATCTCAAAAGTGGTCTAAACCACTTTTTTGTTTTATTTTTAAACAAAAAACTTGATATTTATTATTTTTTTGATTATAATCTTTTACGTACTTTTTAAGTATATAGTTAAACAATTTTAAAAAAAATAAAATATTTCTTGACATTTTAAAAAAAGTTGTTTATACTTTAATGTATTGTGTTTAAAGCTTATTAATAATTAATCACAATAAGAATGTTCTTTGAAAACTAAATAGAATCCGTCAATTTTAAAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAATCGAACGAAGCCTTTTAGGCTTAGTGGTGAACGGGTGAGTAACACGTATCCAATCTACCCTTAAGTTGGGGATAACTAGTCGAAAGATTAGCTAATACCGAATAATAACATCAATATCGCATGAGAAGATGTAGAAAGTCGCTCTTTGTGGCGACGCTTTTGGATGAGGGTGCGACGTATCAGATAGTTGGTGAGGTAATGGCTCACCAAGTCAATGACGCGTAGCTGTACTGAGAGGTAGAACAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGGGCGCAAGCCTTATGAAGCAATGCCGCGTGAACGATGAAGGTCTTATAGATTGTAAAGTTCTTTTATATGGGAAGAAACGCTAAAATAGGAAATGATTTTAGTTTGACTGTACCATTTGAATAAGTATCGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAACGAGCGCAGGCGGGTTTGTAAGTTTGGTATTAAATCTAGATGCTTAACGTCTAGCTGTATCAAAAACTGTAAACCTAGAGTGTAGTAGGGAGTTGGGGAACTCCATGTGGAGCGGTAAAATGCGTAGATATATGGAAGAACACCGGTGGCGAAGGCGCCAACTTGGACTATCACTGACGCTTAGGCTCGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATCATCATTAAATGTCGGCCCGAATGGGTCGGTGTTGTAGCTAACGCATTAAATGATGTGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTTGACAATACACGTAGAACCTTACCTAGGTTTGACATCTATTGCGATGCTATAGAAATATAGTTGAGGTTAACAATATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTTTCGTTAGTTACTTTTCTAGCGATACTGCTACCGCAAGGTAGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATATCTAGGGCTGCAAACGTGCTACAATGGCTAATACAAACTGCTGCAAAATCGTAAGATGAAGCGAAACAGAAAAAGTTAGTCTCAGTTCGGATAGAGGGCTGCAATTCGTCCTCTTGAAGTTGGAATCACTAGTAATCGCGAATCAGACATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGGGAGCTGGTAATATCTAAAACCGCAAAGCTAACCTTTTGGAGGCATGCGTCTAGGGTAGGATCGGTGACTGGAGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTTCTTCGGAGTAAATTTTTAATTTACGTACTAATAAGTGTACATTTTTATTAAAATCCATATGAATATAAGCCACTTTTTTAAAAATATTTAAAAATTCATATGGTCGGATTCTATTTAGTTTTGAGAGTTTATTCTCTCCCATAATAATTAATTTATTATGGATGATCGATCTTTGAAAACTGAATAATTATCAACAAATCTTTCTAATCATTGACATTAAGTTGTCAGTGAACAGAAACTATATTAAATTAAATATAGTTAACAATCATAAATAAACAACTTTAAGGATTTATAATAAGTTACTAAGAGCTTATGGTGAATGCCTTGGGACAAACAGGCGATGAAGGACGTGCTAATCTGCGATAAGCAACGGGTAGCTGATAAGAGGCTTTAATCCGTTGATCTCCGAATGAGGAAACTCAATCTAATGAGAATTAGATTACTGTATAAATGAATTCATAGTTTAACAGAGCGATACCTGGTGAAGTGAAACATCTCAGTAACCAGAGGAAAAGAAAACGAAGTGATTCCCTGTGTAGCGGCGAGCGAAAGGGGAGCAGGCCAAACCGAATTTCGATTCGGGGTTGTAGGACTACAATATGGAATAGAGAATGATAGTAGAATTGGTTGGGAAGCCAAATCATAGAGGGTGATAATCCCGTATACGAAATTATTCTTTTACCTAGTAGGATCCTGAGTAGGGCGGGACACGTGAAATCCTGTCTGAATCCACCCAGACCATTGGGTAAGCCTAAATACTAGTTTGTCACCGATAGAGCATAGTACCGTGAGGGAACGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGAACCTGAAACCATAAGCTTACAAGGTGTTAGAGCACATTAATGTGTGATAGCGTGCCTTTTGAAGTATGAGCCAGCGAGTTATTATAGCATGCGAGGTTAAATCGTAGAGATGGAGCCGTAGGGAAACCGAGTCTTAATAGGGCGTTTTAGTATGTTATAATAGACGCGAAACGGGGTGATCTATCCATGGGCAGGTTGAAGGTGAAGTAACATTCACTGGAGGACCGAACCCACTTTCGTTGAAACGACAGGGGATGACCTGTGGATAGTGGTGAAATTCCAATCGAACTCCGTGATAGCTCGTTCTCGTCGAAATATTTTTAGGAATAGCGTTAGATTATTGGGAATATGGGGGTAAAGCACTGAATCTATGATGGCGCCACCTCGGTGTACTGAATAGAATCAAACTCTGAATACCATATTACCTATTCTAGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCGCGAGGGAAACAGCCCAGATCATTAACTAAGGTCCCTAATATATGCTAAGTGGAAAACGATGTTGAGTTTCATAAACAGCAAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGAGACTCTGCGCGGAAGATGTAACGGGGCTAAGCATATAACCGAAGTTATGGGTTACATATTATATATGTAGCGGTAGACGAGTGTTGTATATGGGGCGAAGGTAGACTGTGAAGACTACTGGACTTTATACAAGTAAGAATGCTGGCGTGAGTAACGAATGAGAGTGAGAATCTCTCAAACCGATTGACTAAGGGTTCCTGGGCAAGGGTCGTCCTCCCAGGGTAAGTCGGATCCTAAGGCGAGGCTGAAAAGCGTAGTCGATGGAAAACAGGTTAATATTCCTGTACCTACAAATTGTGTGATGGAGTGACGGAGAAGGTTATTATGTGCCGGTTATTGGATTCCGGTTTAAATAACAAGGTTAGTAAGTTGGCAAATCCGCTTACTATTAGGACCAAGTTATGAATACGAGCGACCCCTTCGGGCAGTAGCGAAGACATATACATCATGCTTCCAAGAAAAGCTTCTAGCGTTAAACAATTGGTAGTCCGTACCGAGAACGAACACACGTGGTCAAGGAGAATATCCTAAGGTTAGCGAGTTAACTACAGTTAAGGAACTCTGCAAATTAACCCCGTACGTTAGCAATAAGGGGTGCTCGCTGTAAAAGGTGAGCCGCAGTGAATAGCGAGGGGGGACTGTTTAACAAAAACACAGCTCTATGCTAAGTCGTAAGACGATGTATATGGGGTGACACCTGCCCAATGCTGTAAGGTTAAAGAAGAATGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAATGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCAACTGTAGACTCGGTGAAATCCTGGTGAGGGTGAAGACGCCCTCTTGGCGTGATTGGACGGAAAGACCCCATGAAGCTTTACTGTAGCTTAATATTGGGAAATTTTATTACTTGTAGAGCATAGGTAGGAGACTGTGAAGTATACTCGCTAGGGTATATGGAGTCAACGTTGGAATACTACCCTTGTGATAAGATTTCTCTAACCTGCAGCCATGAATCTGGCTGGGGGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCCAAAAGGTAACGGAGGCGCGCAACGGTACCCTCAGCACGGTTGGAAATCGTGTATAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAAGTCGAACAGGTAGGAAACTAGGTCATAGTGATCCGGTGGCTCAGAATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCTGGGGATAACAGGCTGATAGTGCCCAAGAGTTCATATCGACGGCACTGTTTGGCACCTCGATGTCGACTCATCTCATCCTGGAGCTGAAGCAGGTTCCAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTGTCATGCCCGTAGGAAGATTGAGAAGAGCTGTTCCTAGTACGAGAGGACCGGAATGGACACACCTCTTGTGATCCTGTTGTCGCGCCAGCGGCACTGCAGGGTAGCAACGTGTGGAATAGAGAAACGCTGAAAGCATCTAAGTGTGAAACTAACTTCAAGATTAATCTTCCCTTTCTTAAATGAAGTAAGAATCGTTAAAGACTATAACGTTGATAGGTCGGATGTGTACGCACGGTGACGTGTTTAGCTAACCGATACTAATAATTCGAGGACTTATATAAGTTCTTAGTTAATTTTTAGAAGAATAGTTGATAATATTTAGTTTTCAGATGTCGATCATAATTGTGTAGTGATTATATCAGAGTGGAAATACCTGTTCCCATCCCGAACACAGAAGTCAAGCACTCTAGAGCCGAAAATAGCGCAAGTAAAATAGGTCATCGCTACGCTAGAACCAACCTTGAAATTTAAGAGGTTGGTTTTTTTATTTATTATTAATTTTTATATATTATGTAATGATATAATTAAATAATATATTTTTATTTATATGGGGCAACACTATGGCAAAGTATACGATATCAAAACACCGTCTTGAAGAATTACAATTAGAATTAAGAGAAATTTTAGATGTTAAGTGACCAGCAATTACAAAGCAATTACAAGATGCACGCGAACAAGGTGATTTATCTGAAAATGCTGATTATGATGCTGCAAAAAATGAACAAGCAGCATTAAAAAAACGTAAAGATGAAATTGAAGAAATTTTAGAAAATTATGAATTAATTGAAGATGTTTTACGTTCAACTGACGAAGTTTCCATTGGATCGACAATTGAAATTTATAATTATCAAAAAGATTGTGAAGAAGTAATTACACTAGTTGGTTCAATGGATTCAGATCCTTTTGCAAACAAAATTTCAATAGACACACCACTTGGAAAAGCTCTTGTAAAACAAAAAAAAGGTACTGAAGTTACAGTTCATACTTTAGCATCACCTTATAAAGTAAAAATTATAAAAATTATTGATTAATTTAACTAAAAAAAGATTGACATTATATAATAATTTTACTATAATTTTATAGTGTTTTATTAAACACACTATATAAATTGGACAGATAGCGAAGTGGCCAAACGCATCCGGCTGTAACCCGGCTCCGAGAGGTTCGTAGGTTCGAATCCTACTCTGTCCACCATTTATTGGCCTGTAGCCAAGAGGTAAGGCAGAAGATTTTGATTCTTCCATGCGTAGGTTCGATCCCTACCAGGCCAGCCATTAAATTATTTATTTTTTAACCACAATTATTTTCGTTATTATGTTATAATAATTATTGTGTGTTTATGCCTCATTAGCTCAGTGGTAGAGCATTTCACTTTTAATGAAGGGGTCAAAGGTTCGAAACCTTTATGGGGCACCATTTATTATTTTAAAGTAATGCATGGTTAACAATTATACATTGTATTACTTTTTTATTTTTGCTGGAGTGGCGGAATGGTAGACGCACTGGACTTAAAATCCAGTGACTTCACGGTCGTGAGGGTTCAAGTCCCTCTTCCAGCACCATTTGCGGGTGTAGTTCAATGGTAGAACTATAGCCTTCCAAGCTATTAGTGTGGGTTCGATTCCCATCACCCGCTCCATTAATAATATTTAACTAAACTCCACCCTATTAGTGGAGTTTTTCTTTAATTAAGAACTACTTATGCAAAAAAAATACATTAAAAATACTTTAGGCTTTGATACAAATTTATTTATATATCAAGATAAAAAAATGTTCAATTATTCAATTGATACTATTCTGTTAGCAAATTTTATAAGCTTATCTTCTAAGACGAAAAAAATTTTAGAAATAGGAACAAATAATGCTGCATTGAGTATTTTTTTAGCTTTGCGCAAAGAGGATATAAAAATTGATGCGATTGAAATTCAAATAGAAGCAATTAACCTTGCATTATTAAATGTTAGTCAAAATCATTTTGAAAAACAAATTAATATTATACATGCTGATTTTAATCAATATTGGAAAGATTTTGATAAAAGCGAAAATAATAAATATGATGCAATAATCTGTAACCCCCCTTTTTATAAACAAGATAAAATTATTCCATCAACTAAAAATCCATTAAAAACTTTAGCTTTATATGAAATCACGCTTAATTTTGAACAAATTATGCAAGGGTGTGCTAAGATAATTAAACAAAAAGGAAATTTAGCAATGGTTATTCCTACAACGCGTTTAGTTGATCTTTTAGAAATTATGCGTAAATATCAATTTGAACCAAAACGAATTAAAATGATTTATCCACGAATCTATGAACAATCAAATTTAGTTTTAATAGAAGCGCGTTACAAAAGTGGTTGAGGTACACACTTTGAACCAAATCTTTATTTACATTATGAAGATAAACAAAATCATCAATATACTGAAGAAGTTTTAAATTGATATAAACCAATTAAGTTTAAAAAATAATTGTAAGTAAATTAATTAAATTTCTATTTTAAGGATGATGAAAATGAATAATATGGATAAATATAATTTAATTATAGGTAGTCATGTAAGTTTAAAAGCTAAAGATTTCTTTTATGGTAGTGTAAAAGAAGCATTATCTTATGGTTCTAACACGTTTATGGTTTATACTGGAGCTCCACAAAATACAAAACGTCAACCGATTAAATCATTTAAAATTGAAGAAGCACATAATTTGTTAAAAAAACATAATATTAATTTGGATGATTTAATTGTTCACGCTCCATATATTATCAACCCTTGTTCTTCTAAAAAGAATGTTCGTGAACTTGCTAAAGAATTTTTAATTCAGGAAATCCAACGAACAGAATCTATGGGGATCACTAAACTTGTTTTACATCCAGGATCACGTTTAGAACAAAATGAAGATATAGCTTTAGAACAAGTTTATACAATGTTAAATGATATTTTTTCAACTATTAATACTAATGTAATTGTTTGCTTAGAGACCATGGCTGGTAAAGGTTCTGAAATTGGGGTAAATATAAAACAATTAAAAACCATTATAGACAATGTTCATTCTAAAAAAAATATTGGCGTTTGTTTAGATACTTGCCATATGAATGATAGCGGTTTGATTTTAGATTATTATAATTTTAATCAATATTTAAAAGAATTTGATGCACAAATTGGTATTAATTATATTAAAGTATTACATATTAACGATTCTAAAAACCCTTGTGGTGCAAACAAAGACCGCCACGAAAATTTAGGATATGGAACGATAGGTTTTGCAAATTTAATTAATATTATTTACCATCCCTTATTAAATAACATCCCTAAAATTTTAGAAACGCCTTGGTTTAATGTTAATGATGAATTAATTCCTTTATATAAGCATGAAATAAAAATGATTCGCGACTGCAAGTGGTATGACATTAAAAACAAATTATTATCGAAGAAATAAAAACTATTTTTAAATACAAATAAGTATTTCTAAAAAAAAAAAAAAAAAGGAGAAGTTCTCCTTTTTTTCGATCACAATTTTTTATGATCTTATTGGTTATTTAAATAGTTATAACATAACGACTTTTGCTACAACTAGATTGTTGAAGACTAATCTAACAACTAGAAGTGCTCGAATAACCATACAAGCCATTGATATAATAATACACTATTTTTAATAAAAAAGCAATATTTTTATGAATTATTTACAACTTTAAAAACACTTGGCCCTGTTATTTTAAAAATATTACCATATGTGAGATATTCGATACGATTAAATAAGCGTAATTTAGTTTTTTGTTCTTCAATATTTGTTGTATTTTTTGTTGAATTAATTAATTGTTCAATTGAAAAATTAGAAATAAAAATGGTTTGTCTTGTTGATTTTAATCTATTTTCAATAATAGGCATTAAAATTGTTGAAATCGATCAGTAATTTAAATTAACGCTAGAAAAATCATCAATTACTAAAACATCAACATTTGAAAGTAGATTAACTAATTCATTAATTACTAAATCTGATGTATCTTTGTAGTTAAAAAATTGGATTACATTTTTAAATAGATCATTTAAAGTAATATAAGCTATCTTTAAGTTATATTTTAATGAAATAGTATTGGTATAAGCTTGAGTAATTATTGATTTGCCTACACTAAAATCACCATAAATAAAAGCAGATTTTGGTTGTTGATTATTATTTTTAATTAACGTATTTTTTTTATTTTTTCGTGCTTCTTCTAAAAAGAAATTAATTAATTTATTTCGTTCTGCTAATAAATTAGCATCTAATATTTCAGTTTTTTTAGAAAATATTTGACTATCATCTGCTAATGAGATATTATAATCCATATTTTTGATCGTATTTAAAATATAATTTCTTTTAATTTTATCTACTTTATTTTCTTTTTTAACTACTACTAAACATAAGTGATTATATTCATTACGATTTAATTGATAAATGTAATAACTATCATTTTGATTGTTTAAATTATTCAAATAAGAACGATAAACTTCAACTATATCTCAAAAGTATAAATTAAATTCTTCTTCTGTTAAATTAATTGCTTTAATATTCGGAATTTCTAATGCTTTTTGATAATTAAAATTCTCTAAATCTAATTCATCATCAACTTGATTATTGTTCATTTTTATAAATCTCCAAATTTTACTTCAATAAAATTAGTATTTTTGAAAGTATTAGCATTATCACTATCTTCACAAATTAATTCTTTATGATCATTCTTTTTATTTTGAAAAAATTTAATCATTTCATACTCATCTTTAATATTGATTCCCAGTACTGTTTCTTTAATACGTTTTAAATATTTTACATTTAAACGCCCTAAATTTTTAAATAAAACAAAATCTAAGATAACATTAACAATTGGATCACTTAATGATTTGTCATACGTTAATAAATTGATAGCCTCTTTATCTTTTAAACTTAATTTTTCTTTACGAATATGTTTTAAAAAATCTGAAGGTTTAAAATTATTATAATCATTAATTACATTTTTGAATCGATCTAAATTACTGTTTTTATTAAAAATATTAAAATCACGTTCAATATTGTAATATTGAAATTTTGTTTTATTTTTTTCGAATTCCATATTTGAAAAAATTGTTAATTCTAATTTATTTAAATCAATTGTTAAAAAATCAATATTATTAATTAAATTATTTTTGATTAAATTTAACATTTGATCAATACTAAATTTATATTTTTTGAAAAAATTTATTAAGCACAACTTTGTTGATTCATCAATTTGAAGTGATAATCCATATTCTTTTAAAATTATTTTTTTAATTTTATCTAATTTATTTTCGAGATTGTTAAAATGATTTGTCTTAAATTGAGCTGATAAATCAATTAAACGATTAGTATCAATATTATTCTCTTTATTTAATAAATTTAAAACATTATAATGATTTAATGAAACATTATTTTTTAATTCTTCATTAAATTTTTCAATATTTATAAATTCGCTAAATTTTAATGGTCGAAAAACTTCAATTACTTGTTGGGAATTATTTTCAAAAGAATAAATTTTAATTAAATCAACATTAATTAATTTAGTAATTAATTCGTTGTATGTATTAATTGAAATATTAAGTTCATCTAATAAATATTCATGAGTTAAATTAATTGTACCCTTTTTATTAAAAATATTTTCTCTATTAACGAATCAAGAATATAAAGCTACTGCTTGCATACCAATTAGTGGTGTATAAAAAGAATATAAATGGTCATCATTTTTAACAAAATTACAATTTTTCTTAATTACTATATCCATAAACCTTACATATTCCCTTTGAATTTTAATCAAATCTATTTTTCATTAATCAATATTTTAGATGCTTTTATTTAATTTAATTGTCAATTTATTTATTTGATGCTATTAAATATAAACATAATAAGAGTTATGCACACTATTAAAATACTTCTTTTTTATTAAAAATCAATGTTTTATATGTTTAAAAAAAATATACACAATGATGTTTATAAATTATAAACATTAATAAAAAATTCTAAAAAAAATCTAAAAAATTGTGAACATTTTATAAATAAAAAAGATTAAATTATATATTTATAAAACCGTTATTATTTATATAAAAAAAGAGGTTATAAAATGTTTGAACAAGTATTTTTTTTAGCATCCATTAACGACAAAAAGATTATTAGATGATTATATGAACGTTATCATAAAATTTTTTTAAAATATATTAATGAGCAATTGAATTTTAAATTTTATACTTTAGAGATTGAAGCAAATGATTTAAAAACTATTATTTATGAAATATTTTTAAAAATATTTCAAGTAACTAGAATTAAAAGTTTAAATCAATTTATAGGATTTGTTAAACGGCAAATTTATTTTTATTTAGTATATTTATTACGTAAATCTTTATCTAATAAAAATTTAGTTAATCATTTAAATAATAATAATTTTGAAAATTATGAATTAATTAGTACCAATTCAGATGAAGTTGGTGCTGAATTAAACAACAGATTTTTAATTTATGAATTTTTAAGAAGTATTCAGAAAGAAAATTTATTTTTATATCATTTTACTATTTTAATTATTAAAGGATATAATACAAAGGAAATTAGTGAAATTTTAAAAATTAATTCTGCAAAAATTTATTATTATAAAGCACTTTTAAAAAAATATATAAAAAAATTATTATAAAAATACTGGGGTGAAGTTACTTTCATTTCCCAGTATTTTTATAAAAGAATCAAAAGATTTTCTTAATTTATGCTGTGACTTCTAAGTTTGTTTTAAAAGCTTTTGCTGTTCTTGATTTTAAACGATTTGCTTTATTTTTGTGAATAACACCTTTTGAAGCTGTTGTATCAATGACTTTGTAAGCTTCATTAAGTTGAGCTTGTTCATTTGAACTTCTTGTTTTTTTAACAATTGTTCTTAATTTAGACATTTTAGCATGATTTTCCTTACGTACTTTTTGCGTATGTCTGTATGTTTTTTCATTTGAAACAATATTTGCCATATACTCGCCTCCTACCTAATATTTGAATTCTAAAGTTTAATTTCATGTTTGAATATAACTTGTTTATTATATCAAAAATAATAAATAAAAAAAAATAAATGTTTTAACAGATTAAATTTATTTATTAAAAATAAGATGAATTTTCTAAAGCTTTCATAACTTTTAAGTTATACTTTAATGGTGTAAAAAGCTAATTGCTGCATTTTTTAAAAAATGTAGAGGAAAGTCCGTGCTCACACTAACTGTGATGTTAGTAGTGTTTGTGTATGTATAATTAATAAGGCATAGCATAGGCATATGACGGCAAATTTTTAAGCTAAGTGATTTAAATTATATGTTTAAAAATTTATAAAAGTGCCACAGAGACGATTAATCAAGAAATTGATGAGATGAAACGTTGGTAAACCCCACAAGTGAGAAACCTAAATTTCGGTAAGGGAATCTAATTTAGTAAATTAAGAAATTAAATTAGGATAGAATTGTTTACATTCTATAGATAAATAATTAGCGCTACATTTTTGTAGAACAGAACACGGCTTATTTTTACATGCAAAAACCCACTCTTATAAAAAAGAGTGGGTTTTTGTATTATGTTTGATTATATTTTCAAATCATTAAAATAGCATCAGAACCATTGGCATAATATCTTTTGCGAATGTTTTGTTTTATAAAACCATGCTTTAAATAAAAATTTAATGCATTTGTATTAAGATCGCTTACTTCTAATAATATGTTTTGCTTTTTTTGTTTTAATAAATCAATTAATAAACTACCAATTTTTAAATTACGATAATTTTCATCAACACCAATTTTTAAAATTTCATTAAAATCAACACTCTTCATATAAATTAAATAAGCAATGTTTTTTTGCTCATATTCAACAATTATTATTTGATAATTTGAATTATGAAAAATTTCTTCTAAATTTCTCATCGAATAACAATCATTTAAGAAAAATCTATTTTCAAAAATAGCAATATCATTCAAATCTTTTTGATCAGCAGGTCTAATTAAGAGGTTTTTTAACATAATTTAATCTAAAATCTAAGATATTAGTTAACTTAAAATTATCAAATTTTAAGTTATATATGAGATCATGATAGTCAATTTGATTTGCATCAATTATTGTTAATGATGTATTTTTTAATTCATTTTGTAATTCTTTATTAGTAATAATTTTATATTCGTTTTGAATATTATTAGTAATAGTTATTACATACGATTTATTACCTTTAGCATCTAAATAAACAAAAACATCATTCTTACCATTGGCTATAATATTTAAACTATCATTAATAAAAAGTTTAATTTTATTAAAAATTGTACAAATCGTTTTAGCAACAATAGCACCAACACGTATTCCAGTAAATGATCCTGGACCAGTATTTAAATATATTGCTTCTAGATCTTGATATTGCAAGTTAGTGATTTTTAATAAATTATTAAGGTGTTCAACAATAATATCTGTAAGATTATTGTTTGTATCAACAATAATGCTAGTATGGATTTTAAAGTTTTTATAAATTGCTAAAATACATTTTCTTGATGTAACATCAATAAACAATTGGTATGTACTATTCATTTGCTTTTAACTCTCTAATTAATTCCATAAATTCTTTTGGCATATTGATTTTAAATTCTACTTTTTTATTAGTGGATGGATGATTAAAACTAATATAAAAACTATGCAAGTATTGCTCGTATTCTGTGGTATGTTTACTAATCCCATATAAAGGATCATTATAAACAGGGTGATTAATATAACGCATATGAACCCGTATTTGATGAGTTCTACCAGTAATAATACTTACTTCAACAAGGGCGGTATTTTTATATTGCTCAAGTACTTTAACGATACTGATAGCCTCTTTAGTATTTTTAGAATTGCCTGTTTTCATTCTTAATTTATCTTGATAACTATGGCCAATAGGCTCATTGATCATAAAATGCAAATGCTGCATATCAAAACGATTATGAACAATAGCATAATAACTTTTTCTTATGGTTTTTTGCATAAATTGTTGTTGCAAAGAATTAAGCGCTATTTGATTTTTACAAATTATAATTAATCCAGATGTATTTTTATCCAAACGATGTGTAATATAAAAATTATCAGTTTTTAAATAATATTTTAAAGCGTTTGCTAATGTATTTTGCTCATGAAAACTAGTTGGATGAACTAACATACCAGAAGGTTTGTTAACGATCAATAGATCCTGATCTTCAAAGATAATTTCTAATGAATATACAAACGGTAACAATTTTTTTTGCTCTTGCACTATAGGTAGTTCGTTATTGATTTGAATATAATCATTCTTTTTTAATAAATAACTTGCTTTACTAATAAAATTGTCATTAACTAATATTAGGTTTTGGTCTAACATTTTTTTAATTAATGTTCGTGAATAATTAGTATTTTCTACTAAAAATTTATCGATGCGTTGAGACGACATTAAAGAATATAGTATTTTCATTGCTATGATTTTTTTAAGTCATCATATCCGAATGGTTTGTTAGAAATTTCGTTGATTTCTAAATCATTCTCATCGGCAACTTTTTTTATAATGTCTTGATTATCACTATCATTTTTTCATTTGATAATAAATCTAATAACTAAAATAACAACTAATACACAAACACCAACAACAATATAAACATCAAAAAGATTAATTATACTATTAACAAATTTTCAATAATCAACAACACCATTAATAACATCATCGCCTCCACCAACACTATTAGGATTTATAAGGCTATTGTGAAATATATTTGGTAAAGCATTGAATAATTCAAATTCTGGAATTGAGCGATCAATAATATTTCCTAAACCGCCAAATAATAGAAAAAGCAATGGAACATAATAACAAGAATTTTTTAAAAAAATAAATGATAAAAATCCTAATATGATGGGGATTGATTGTAAAGTATAAACAATTCCGCTATGGTTATTAATTCTACCAAGAGCAATCCCATTATTAAGTGTAAAATGAAAAATAGGACCATGATGCGTTTCTTTTGTTTTGACAACTAAATCAATAGCTCATTCACGAACCAAAAAACCTGATAATAGCACAATTAAAGCAACAATAATAAAATTAATTATTTTATAAAAAATTACATCTAATGATGTTATTGATAAAAAGTAATCACGAATGCTTTTATTGTTTATTTTTAAAATAATTTTCTCAAAAAAATTTATTTTTCTAAGTGATTGATTAAATTTTTTAATTATTATATAAAAATTTGAATTTTTGATTTTTTGAAGCATTATTTAATGTACTTTTCTGGACGTTCTTTCATCATTTTAGTTACAAATTCATCTTTTTTAAGATGGCCATATTCTTTCATTAAAAGAACACATTCTTCATATTCTTTTTGAGCTCATATTTCATCTTCAAACCCCAAGATTTTAACTTTTTTATTTTGTAAGTTTTTGTAATTTTCAAAGAAATCTTGCACTTCTGCTAATCAATGTAATGGAATATCTTTGAAATTATTAATGTGTTTATAACGAGGATCAACATCAATAACTGCCAATAATTTAGTATCTGTTTCTCCTCCATCAATCATTTTCATGGCGCCCAAAACACGTGCAGGTACTTTAATTCCTGGTTGGAATGCTTGATCAGCAAAAACCAAAACATCCAATTCGTCACCATCATAATCTAATGCTTCTTTTAAAAAACCATAATTATGAGGATAAACCATTGAACCGTGTAAGATACGATCAACCATGATTTCTTTAGTTGCACGATCATATTCATATTTAATATTTGAGTTCTTTGGAATTTCAATAGTTACATTTAATTTCATACTACCTCTCCTTTTTATTAATCTAAATATCCTTAACTAATATTTTATAATTATATTATGTTTGTCTTATTTTATATTTGATAAAAATAATTGATTACCAATAAAATATACTTAGAAATAATTAGTATCATTAACATAGAAATAAAAATAGTAAGGATATAGAAGATGAAAAATTTTTCAAAAAATAGAAATATTAATAATTTTAAAAACCCTTTAAGAGTTATTGAAGGAATTGTTCGTGAAATTAAAAATAATGAACTTCAAATTGATTTTGTTAATAGTCATAAAAAAGGAGTTTGTAAGTTTATTAATTTGACAGATTATCATTGAAATACAATTTCTTCGCGTTTTTTAATTAACAGTAAACACTTATTTTTAGTTTCAAAATTTGACCCAATTCGTCATGTTTATTGATTAAATTATAAAATTATTCATCCTATTGAAATTAAAAACAAACGTCGTTCTTACCCCACTTTATCTCATGATAGAAATTTACGGATCTTTTTAAATAATCTATTAGAAAAAGAAAATGAATTAAAAAACTAAAAATTTTTAACATATGAATTAAATTTTAAAATCAATTTATTTCATTAATTTAGAGAATTTTAATTATCAAAAAGCATTAAAAATTATTTTTATGTTCTAATAAATTTTATAAACATAATAAAAAAATAATTTATTGAAAAGTATAATTTTAAACAATGAAAACAAAAAAAGAGAAGATAAAAACATTTTTTTGCTTAAAATTATTATTATGTTTAGAAATTTAATTAAGAATGTCTATCGTTATTTGCTAAAAAGCAAAGCCTCTTTTATAGGTCTTTGCCTTTTATTGTTTGTGTCAATCTTTAGTTTTAGTATGTTAACAAATCTTTCTAAAACATTAACAAGATCATATACAAACTTAGTCAATAGACAAAAATTACATGATATAACTGTTAATGAAAGTTATATTGAAGATCAAAAAGATATTAAACAAGCTCAATTCAATAAGCTTTTAGAAGAATTAAAAGTTTTTTATGCACAAAAAGGTTTGAATTTTGATTGAACTCGTTCTAATTCATTGATTGTTAATTCGACAAAAGATGATATTACTTATAAAATTGTTCAGTATTCAACAAGTAAAACGATTAATACATTCGATAAACAAACAAGTGAACGTTTGATTAAAAATAGTTTTTCTGATATTGATTTATATGGGATTTTAAATAATGCTAAAGTAGATGTTATTGATTTAATCAAAACTAAAACTTTAAATGCCAGTTTTGTCATTAATAATTTAAAAGAATTAGACAATAAACAAAAAAGTAATTTGGTAAAATTAGCTAAAGTTGATAATTACGATGTTAGTATTAGTCAACAAGCACGACTTTATTTATTAAAAAATATTGTTTATGGTGCATGACCATCAAAAACCAATAATTTATATAAAAAATTTAAAAAAGCTTATGAATATAGTCTAGAAGATAAAAATTACGATCCTTTAATTGGTAATATAAAATTAAACAACAAAGCTAATTTTGATATTTTAGAAGCAAAAGAGATTAGCACACAATTCATTTTAATGTTATATCCATCAAAAAAAGGTATAAGTATAAATGGTAATGTTAATACTATTGTTAAAGGGCATCGAATAACTTTTAGTTTTGAACCCGCAGGGATTCCTATTCCAGTCTATTTTGATGTTAAATCTGCATACGCATTAGTTGGTTTAGGTGCTTTTTTGTCTAATCATAACAAACAAAAATACCCAATGTATTTATGACAAAAAGCTTTAGTCGACCACGAAGAACAACAAAGTTTTGAAAAGTGATTCAATAGTTTGGATGAAAAATATATTATTCATATTGATAACACACCTTATTTAATTTTAGATAGTGGTATTACTCCTGATTTTATGTATCCGATTATCTCATTCACTTCTGTTATTCCTAATCCAAAAAAAGAAGGACTAGTATATGTTAACTTAAATGGATATGCCCGTATTTACGATGCTTTTAGATCTAATCCAATTGAAAATAATTTACAAATTCGTATCACAAATCAAAAGCATCAATCCGTTGATATCAGTATTTTAAAAAAAATTGTTAAAAATTTAAATGATCAAATTAAAAAAGATGCTATCATGAACTGACCAACAAGTTTAGATGCTGCTTATTTAAAAGATGATACTAATAATCATATTACACCAAGCCCATTAAGATTAGTTTTCATTAACAAAATTACGAACACAATACAAACTTTTAGTGTTTTAATAACTTTATTTATTTTGATTCTAACAATTTTTGTAATTGTTATCATCATCAACCGTTTTTTAGCACAAAATAAAATTAATATTGGTATTTCTATTGCTAATGGTGTTCCTCGTTGAAAAATCATTTTAAGTTTTGCTTTTATTGGATTAATTCCATGTGTTATTGGTGGATTATGCGGTTATTTAACAGCTTATTTTACTCAAAATTTAGCAATTAATATTTTTAGTGGTTATTGATTAATTCCTACAGTTTTAGAAAACTTTAATTTTGGTGCTTTAATAACAGTTATTATTTTCCCTTTTTTGTTGTTTTTTATTTTAATAGTTGGATTAGGTTATTGATCAATAAGAAAACCACCAATTGATTTAATGCGTGGAACTGAAACATTTAAAATTTCTAAAATTGTTTATGTAATTAAATTTCCATTTAAATATATTAGTGGTTTTAATAAGTATTTAATTACACTATCTTTTTCATCAATTACAAGAATTATTATTTTTTCAATTATGATTGCTTTATCATTTGGATCGATTATGTTTTTAACTGGAACAAGAGGTAAAATTAAACAAACTATAGATGCAACATTACAAACAAAAAAATATCGTTATGGAATTGATTTAATAACACCAACAGATCAAGGTGGTCAATATATTTCTTTAAGTACAAATCAAATTGGTTCTACATTAAATGATAATGATAATCATGAATTGACACAGAAATATGAAAGTAGTGATTATAAAGATTTTATTAATTCACCGTTATTTAAAAATTTACAGAATTTGAAGTTAATTGGTGCAAAAGATGACCAGCGTCGAATTTATGATTTAAACTATATTGCTAATGCCTTTCAAGTTAAGCAATTTTTAGATTTTAATTTTGGCATTGGTGATGTTAATAGTGGTAGTTCAAGTAATCCATGAAGTATTACAAAGTCTTTAATACCTGAAAATAATTTACAAATTTTAAATGAAACTTATAAAAAATGATGTTCAAAATTAATTAATGACCATAGCATTTTTTCGAAACAATTAATTGAAAAAGGTGTTAATGAATTAGCTTATGATATTTTAAATATTCGTAATAGTTATGATCCTAATGAATATTTTAATTATTTTACATTTAAGAGTATTGTTGATACATATTTAAAACGATTTAATAAGTCTTTTTTAGATCAACATGTTACGTATGAAATTTTAGTACGCGTGCATAATAATTTATTTAATTATAAAAATATTTATGGAAAATTGGTTTTTAAAAATAATCAATGAATAATTAATTTTAAAGATAATAATTTTCCAATTGATGATCAAAACTTTGTTAGTGCTATTTATTATAAAAAACCTAATGAGCAAAATTATGATTTAAAAAAATATATTTTTAAAAATGATGATTTGATTTATGATGAAGTTTATTTTGAACGTAATAGCAAACAAAATTATCATAAACTAACTTATCAAACTTTTAGTTCTTATTTTAGAGCACGTTTTTTAAAATTTGATCCAGAAGGGGATTTTGATTATTATTTACCATTAAAATATTTTAATGCAACAATTGTTAGAAAAGAATTATTAGAGAATGCAATTAATAATGAAATTCTTTTTAAAAATGACGAAAATAAAATCATTAAAAAAATTAATTTTAAAAGTGATAATGATATAGTTGTTACTAAAATCAATGATTTAACTTATCAAGTTAAAAATATTATTAATCAACAGATTTATCTTTATTCTCCAAAACCCATTAAACGTTCTTATAGAATTGATCCAGCAAAAGCTACAAATGCTATTAAAACAGGATTATCTATTAAATTGGATAATGATTTTATTAATTTAGTATTATTAACTCATCCATCAATTAATCCATCGTATGCAGATGTTTGGTCAGGTATCACATTTAATATGGTAGGTTTTGATTATTTAACAAAAAATGATAAAACACAGTTAAACGAATTTGATGAACCTTATATTTGAATTGATGCTCAGATTAATAAAATTAATAATCAAAAATATGAAGAAGCTAAAAATCCTAAAATTATTGGAATTATTAAGAATTCAAAGTTAGTGCATTTATTATCCAATAAAAAACAGATTAATAATTTATTATTTAATAAAAATGATGAGCAAGTAGCAATTATTAATCGAGTAGCTGCCAAATATTACAATTTAAAAATTGGTGATCATTTTAGTTTTGTTGCTAATAATCAAACAAATCGTTATACCAAAGATTACATAAAAAAACAAATCACTTTAAAAGTAGTTGGTATAATTGATACTTATCAGGGGATCGAAATTTATACAGGGATTAAAAATGCCGCTGAAATTTTAGGAATGCATAGTTCGCCATATGCTGATAAATCACTTTATGATGAATACGATGGTGCTTTCAATGGAATTTTTACAAATAAAAATAATCCAGCAATGTTAACACGTATTATTTCTATTTACTCACCTTCGGGACTATATCCTATTTCTGAAACATGAAAAAAAGATGCACCAACTATTAATCTAATTAAAAATATTTTAAGTGATAAATATACTACATATGAATTAAATAATAAATGAGATCCCAAATCATTAGTTTTAAACTCTCGCTTATCTTTAACTAAAGCCTTAGGTTATAAAAATTTTGATGAACTTAAATTAGATGCACGCAATATTTATGATGACAACCATAATTATTTAGGATTAAGAGATGAACAAGCTCAAGCAGAATGGGTAATTGATAAACTAATCAGTATTTATGGGCGTGATACTTATTCATCTACTTTAAATAATGTTGAAGTAGTTGACGTATTATTTGCAGTTTTAGAAACTGCTTCAAAAACAATTCAAACTGTTGAAATTGTTGTGATCTTTTTAATATTATTTATTATTATATTAGTACTTGTTTTAATTTCTATTAATTCATTGTCTGATACATTAAAAGTCGCTAAATTACTAAAAAATATTGGTTATAGTGATTTAAAGAATGCTAAGTTATTTTTAATGGCTTTTTTACCATCATTAATAATTGGATCAATTATTTCAATTCCACTAGTTATTGTAGTAATGCAAGTATTTAGCGAAATAATATTTAATAGTTTAAATGTTTTATTAACAACTAGTTTTATTTGGTGACATTTTTTAATTATTTTATTAATTGTTGCTATCATTTTCTTATTAATGTGAGCGATTGCAATTTGAATCTTAAGAAGATCAAATATTGCTGATGCAAGTAAACGTAATTAGAAATTTAAAAAATATTTAGAATGTTATGATATAATTTAACCAATAAGTAAAATGTGAATGAAATGACGACCTTAGGGTTGTCATTTCTTATTTATATGAAAGGTTATAAAAAATGTCTAACTCATTTAAATCAAAAGAATTTATTGAATACTTTAAAGATACAGCAAAACAAAATGAAATTGAATTTGAAGTTTTATCTTCAATTATTAAAGAAGCATTTGAAAAAGCTTATTTACGAACACATCCTGGTGAAAATTTTGAAACAAATATTGATCTTAAACAAGGAACAATTAATTGTTTTCGAAATTTAGTGGTCGTTGAGAATGAAAAAGTACATAATGAAGATTTAGAAACATGTTTGGATGATGCTGTTGAAATTTTATTAGATGATGCTCGTAAAATCAATGCAAATGCTCAAATTGGCGATCTTATTAAACAACATATTTCAATTGATAATTTTAAAAGTATTGAAGTAGGTCAAATTGGAAGTTTATTGCGTCAAAAAATTACTGAAATTCATAATAAAAGAGTAGCTGATTTTTGAAAACCATCTTTAATGAAAATGATTCGTGCAAAAGTTGCAGAAGTTAATTATAACAAACAACGTAATGAAATTACTGGAGTAAAAGTTGAACTAGATGACCAATGAAAAACATTAGGTTATTTAAGTCGTAAAGATCGTATTGGTGATGAAAAGTTTAAGGTTGGTGAAACTTATGATTTTCTTATTAAAGAAGTTAAAGAACAAAGCCGTTTATGGCCAATTTTACTATCTAGAACAGAGCCAGAATTAGTTGAAGAAATTTTAAAACGCGAAGTTGTTGATATTAAAAATGGAAATATTGAAATCAAAAAAATTGCTCGAATAGCTGGTTTTAAAACTAAGGTAGCTGTAAGCACTAATTTATTAAATATTGAACCAGTTGCTGTTGTTGTCGGAAATAAAGGTTTAACTATAACGTCAATTTCTAAACAATTAAATAATGAACGTATTGATATTATTCGTTATGCTGATGACAAACGTATTTTTATTGCTAATGCCATTGGTTTAGATAAATTAAAAGGACTTTTAGTTCAAGAAAATGAAAGTGGTCAACGTTCTGCAATTGCTATTGTAAGCAAGGATGATTTACCAAGTGTTATCGGTCGTGGTGGAGCAAATATTCGTTTAATTGCTAAAATTACTGAATGAAATATTGATGTAAAAACAATTGAGCAAGCATTCGAAGAAAATATTTTATACGAAAAATTTGATGAAAAAATTTATCGTTCATGAAATATTGAATCAATTAATAAAAAAAATGTCACAAATGATGAAATGTTAGCTTTAATTGATAGTATAGAAGATGATAAAATTGAACAAGATGTCCAATTAAATCACCCATTAAAACAACAAGAAAAAAAAGTTGTTTCTTCAAATGATGAAGATGGTGAAAATGATGAAACACAATTAGAATATCTTGAAGGATTCGAAGATTTTAAATTCTAATTTAAATATTATGGCAATGAAAGTTAATGAAAGAACTTGTGTTTTTAGTAAAGAAAAATATCCTAAAGATCATTTAGTTCGCTTTGTTATTATTAACGGTGAGTTAATTTTTAGTTTGAATTATCATCGTGGGTATTATTTAATGATTCAAAAGAATACAAACATTCAAAAGGTGTGTCAATTTCTAAAAAAACGTTTTATGATTAAAAACGAAGAACAAGTTTATCGGATTTTAGAGCAATTAATTCAATCATTAATTTAAAATATATAGAGTAATATAAGTAAGAGGTAAAAATATGGCGAAAAAAAATATCAAACAAAAGAAAGATAATCGGATAGCAATCGATGTAAAAAAACACATTAAAAAAGTTGATGTTGGGGTTTTTGATGGTACATTTGTTTTCACATCACCTCTTAGTATTTCAGAATTAGCACCAAAACTAAATAAAAGTCCAAACGAAATTATTATGCGTTATTTCAAAAAAGGTGTTGTTTATAACTTAAACACTATTTTAGATGAAGAACAAATTGGTGAGTTATGTTTAGAATATGATTTAGATTTTAAAATTGAAAAGAATGTTAACACTGAAAATCTTTTAGAAAATATTTATTTTGATGATCTAGAAATTGATTTGGTTGCTCGTGCTCCAATTGTAACAATTATGGGTCATGTTGATCATGGTAAAACAACACTTTTAGATACTATTCGTAAATCATCAATAACAGCATCAGAAGCTGGAGGAATTACTCAACATATTGGAGCTTATCAAATTATAAAAGACAATAGAGCAATTACTTTTATTGATACACCCGGACATGAAGCCTTTACTGAAATGCGTGCTCGCGGAGCAAATTTAACAGATATTGTGATTTTAGTTGTTGCTGCTGATGATGGTATTAAAATGCAAACTGAAGAAGCAATTGATCATGCTAAAGCTGCTAATGTTCCTATTATTGTTTTTGTTAATAAAATGGATAAATACGAAGCAAATCCTGAAAAAGTTTTAAATCAATTAAGTGCTAAAGAAATTGTTGCTGAAGAGCTTGGTGGTGATGTTGTTTTTGTTAAAGGCTCAGCATTGAAAAATGAAGGAATTTCTGAATTATTAGATTCAATTTTATTGATTGCTGAATTAAATAATTACAAAGCTAATCCTAATCGTTTAGCTTATGGAACTACAATTGAAGCAAATTTGGATAAAGGTCATGGTCCTCTTGCTACATTGTTAGTTCAAAATGGCACATTACGAAAAGGTGATTATTTAGTTGTTGGTTCAACATATGGAAAAATTCGTAATATGTTTGATGAATATGACAACGAAATTGAAATAGCATTACCTTCAAAACCTGTTAAAGTTTCTGGATTTGAAGAAGTACCAACAGCCGGTGATAAATTTTTAGCTTTAGCTGATGAAAAACAAGCACGTGCAATAGCAAATGATGTTAAACAAAAGAAAATGCGTTTAGAACGTGCAATGTTACAAAGTTCGGATATTCGAACAAAAATTGCTAATGGTGAATTAAAAAATATTAATTTAATTATTAAAGCTGATGTTCAAGGTTCACTTGAAGCTTTAAAAGGAATTTTTAGTAGCATTAACATTGAAGGTGTGACTACGACACTAATACGGAGTGCAATTGGAACAATTAGTGAAAGTGATGTGCGTTTAGCACAGACTTCTGATGCAATAATTATTGGTTTTAATGTCCGTGCTAGTCGAATTATTAAAGATTTAGCTGATTCAGTCGGTGTACAAATTATGAATTATGACATTATTTATAAATTTAAAGAAGATTTAGAATTATGAATGAAAGGTACATTAGACCCAATTATTATTGAAGAAGTGATTGGCGAAGCCAAAGTTCTTAAATTATTTAAACACAGTCAAGTTGGAACAATTTGTGGTTGTCGAGTAATTAATGGTAAAATTAAAAGAAATGCTTTGGTTCGTGTTTTACGTGATGGTATTGTTATATATAATTCAAAAATTGCGACATTACAACACAATAAAGATAGTGTTAATGAAGTTATTGCTGATAAAGAATGCGGATTAACAATTGCAAACTTTAATGATATTAAAGAGAACGATATTATTGAAGTTTATATTAAAGTTGAAAAGAAACATGATGAGGTTAAATAAATAAAATATGGCAAACGAAGTTCGAGTTGCAAGGTTAGAATCTTTAATTAAAGATGTTATTAATAATGCGTTAGCGAATGAAATTAATGATAAAATTGCAAAATTAGCACGTGTAACTGCAGTGCGTTTAAGTAATGATTTATCAGTAGCAAAGATTTTTTTAGACGCTCATAAACGCGAAAGTATGCTTAAAGTTTTAGAAAATGTTAATAAAGTTAGTGGCTTATTGCGTTCAAAATTAGCTGCGGAATGAACTAGTTATAAAGTACCGGAATTGCGCTTTGTAATCGATGAAACTATTGATTATGCTAATCATATTGATGAATTATTTAAAAAAATTAAACAACAAGAAAATTAATTATCCAATGTTAAAAAAACAAAATAAAAATAAAGAACAACACTGATTAGAAAAACATTTACGACAAAAAACAGGTTTGATTATTTCTTGATCAATTATTTTTGGTGTTTTAGTTTTATTATCAATTGGTTTTGGTTTAATTCTACATTTTTTTAACTCAAATAATTTAAGTATTCAATTAAGCTTTATTATTAATCTCAATAAATACTTAGTTAATATTACTAAAATATTAGATTATATCGGTTTTGCTTTAATTTATTTACCAATTATTTTTTTATTAGGTTGTTGAATTACTGGAATTAATGGCGTGCACGAATCATTATACTATCATGTGTTTATTTGATTATTTTATTTCATTAGTGTTATTTTATTAATCATTACCATTTGTTTAAGTATTGCGACACACATATATTATTAAAAAAGATAGGGTCATACTCCTATCTTTTTTAATTAATTTTTTATTGTATTTATCTTAATATTATAATCATTTTTAAATATTAAAATTTAAGTTGATTGTTGTATATTTTTGCATTTTTACAATATTTTAAAACAAAGTCCCAAAAGGATTTTTGATGATGTTGATATTTTATGTGGGCTAATTCATGAATAACAACATAATTAATGATATCTTGATCATAATGAACTAAAAAATACGAAAGGCCAATCGTGTGTTTTATTTTATTATAGACACCTCATTGGCTTTTGTAAAAGCCAAATACATATTTTTTTACAGTTAGAGTAGTTATATTTTGGGCATTTTTAATAATTTGTGGAAAAATTATTTCTGCTAGGTATATTAAAATTTTTTGATAAAGCAATTTTGTATTAGTTAAATTCTGATTTTGATCATAAAGATAAATGATTTGTTTTTGAAAATTGAATTGACAATGAAAATTGTTATTTTTTATTTTTATAGTCGTAAATTTTATTCCTAATAAGATGATTCAATCTAAAGATTGTTCATCATACTTAATTTTCTTTAAGTCATTTTCATATATTTTTAAAATTTTTAACTTATTATTTTTAATAAATTCTTCAGCAATATTAATTAATTTTAAGTGATTGATCAAACAAACAATTTGATGATTAATGATTTTGATTTTAATATATTTAATTCTTGGTTGTAAATAAATTTTAATAAAATATTTTTTTTGATTAATTTCAAAATTTAATTCTTTAACAAAACTTAACATAAATAATATATATTAGTTTTCACTTAAAACTTTTACAAAAGCATCTTGAGGAACATCAACATTACCAATCGCTTTTAGACGTTTTTTACCTTCTTTTTGTTGTTCTAGAAGTTTTTTCTTTCTTGAAACATCTCCACCATAGCATTTAGCAATCACATCCTTGCGTACTGCTTTAATAGTTTCACGAGCAATAATTTTTGAACCAATTGAAGCTTGAATAGGAATTTCAAATTGATGTTTTGGAATAACCTCTTTTAGACGTTCACAAATAATTTTTGACTTCCCATAAGCAAAATCACGATGAGCAATAATACTTAAAGCATCAACTTTATTACCATTTAACATAATATCGATTTTGACTAATTTTTCAGCTTGGTAACCAATTAGTTCATAGTCCATTGTTGCATATCCTTTAGTAATTGATTTTAGTGAATCAAAAAAACTGTACATAATTTCAGCTAATGGCATTTTATAAATTAATTTTCGTCTTGTACCGTCAATAACTTCTAGATCATCATATATTCCTCTTCGGGACTGACATAATTCCATAATTGCACCAACATAATTATCTGGTGTAATGATTGCTAATTTAACAAATGGTTCTTTAATAAGTTTAATACTAGTTGGTTCAGGCATTTTAGCAGGACTATCAATACTAATTTCTTGATTATTTGTTAATTCAATTTTATATATTACTGAAGGGGCAGTTAAAATTAATTCAATATTAAATTCACGAGCAATTCGTTCACGAATAACATCCATATGTAATAAACCTAAAAATCCACAACGAATTCCAAATCCTAGTGCTTGCGATGTTTCATATTCATAAGTTAATGCAGCATCAGATAAGGAGATTTTAGCCATAGCTTCTTTTAAATCATCATATTGACTAGTATCAATTGGGTATAAGCCACAATAAACCATTGGTAAAATTTTTTTATAACCTGGTAGTGGTTTATCAGCAGGATTATTGGTATGAGTAATAGTATCACCCACATTAATATCTTTGACTGTTTTGATAGCTGCAGCTACCCAACCAACTTCTCCGGCTACCAATTCTGTTTTATTAACAATTTTTGGTGTTCTAATTCCTAATTCACTAACAATATAATCTTTATTGTTAGCCATCATACGAATTTTATCTCCAACTTTAATTGTTCCTTGTTTTACACGTACTAAACAAACAACACCTTTATAAGAATCATAAAAAGAATCAAATATTAAGGCTTGTAATTTAGCATCATCTTTAGCATCTAATGGAGGTGGAACGTGTTTAATTATTGCTTCTAATACATCTTGAATGTTTAACCCCGTTTTTGCTGAAATTAAAGGCACATCCGCTGTATCTAAACCAATAACGTCTTCGATTTCTTTTTTAACACGATTAGGGTCTGCTGATGGTAAATCAATTTTATTAATTGTTGGAACAATCTCTAAATTGTTTTCTAATGCTAAGTAAACATTAGATAAAGTTTGGGCTTCAATTCCTTGTGCAGCATCAACAACTAGAATTGCTCCCTCACATGCTGCTAAACTTCGTGAAACTTCGTAAGTAAAATCGACGTGACCGGGAGTGTCAATTAAGTGAATTAAATATTCATGTTTATCTTTAGCATGATATTTAATTTGTACAGCATTTAATTTAATAGTAATTCCACGTTCACGTTCTATATCCATAGAATCTAAAATTTGGTTTGTCATTTCACGTTTACTCAAAGTATTTGTAAATTCAATAATTCGATCAGATAATGTTGACTTCCCATGATCAATATGGGCAATAATACTAAAATTACGGATGAATTTTTTATCCATATAACAATCACTCTTTACTTTTTTTAAAAATATCAATATATTTTAAATTATTTTAAAGTAATAAAAATAAAAAACAACTCTATTTAAAGAGTTGTTTCATGTTTTTTTGATTTTATATTAAAAATCTGACAGTGATTTCATATCTACATCTTTACGCTCTTGTTTGTTTGCAGCATAAATTGGGAAAGTTTCAAGTTTTAATTTTTCAGCAACGATAACAGTCATAAATTGAAAAATCATAATATTTCAATCAGTTGCACGTGAGTTATAAGCACGTCGTGATGAAGCAATTTCATTTTCTAAATAACTTGATGTACTCATCAATTCCATTAAAATATTGGATGATTTCAAATCTGGATAACGTTCGAATTGAACATTAACTAAATTTTGAAGTTTATTTAATACTTCTTCATTTTTGTTAATATCATTTGACATTTGAATACTTCGATTTTTAGTTATTTCATCTAATACTTTTTCTTCATATTTGTAATATGATTTAGTTTGTTCAATCATTTTATTTAATAAATCGAACCGTTTTGTTTGTGCTACTTGGATGCTTGATGAAGCTTCATTAACACTGTTTTTAGTTTGAATTAAATGATTTTTAGTATTAAATCATCAAACTAATAAAAAGATTCAACCAATAATTGAAATAATTGAAATAACTATGATGAAAATATATAAAGTTTTTTCGCCATTTGAAGCAACTACTACTTTTCGTTCGTTAGATACATTAGGGCTAATTCCATTTTCATCTTGTTCTTTTCATAAATCCATAAACTTTGCTCCTTATAATTAAATGATTTTAATAATCAATTAAAATTATATAATATAAAAATAGTAAAAATTTTTCTAACATGCTTAGTATGTTAAAAATATTTACGACAATTTAGCGAATTTTGCACATAATCAAGGAGAAAATATGGAAAAGACAATAATTCAATTGGAATTTAATGAAGAAGAATTAGCATCAATAAAAAAAATTAAAGATAAAATGGATCCTAATAATAATTTAGAATTAAATGTATTTTTAAAAGATTTAATTAAGGATTTAGCGCGTGATTATTTAAATTTTTCAAACCAATCATTTGAAAGTATTGCAAAGCAAATGAATGATTTAAAAGATCTAATAGGAAACGTAGCGAACGATCCATCTAGTTTCGATTTTAATTCAGTAATGAATGAATTCCAAAAGTTTAGTAAATCACAAAAGGATGAAGAAAAAGAAATAAAAAACGATACTGAAATAAAAACTGCGACTAAACCTACTAAAAAATCTTAATTTATGTTAAAAATTATTACATCTAATAAAAATGAAGAAATTATTCATTTTTATCGTTTAATTCATGATAAAAAATATCGCGAAGCTAATAAATTATTCATAATTGAGGGTTTTAAATTATTAGATGAAGCTTTGCAAAATCAACAAACAATAGTTTCAATTATTATTAATGAACATGTTTATAATAAAGAATTTGAAACGATTTTTAAATATACATTATATGACAATATTCAAATTTATAAAGTTAGTGGAAATATTATTAAAAAACTATCAGTTAATATCGAACCAAGTCCAATTTTAGCAGTTGTTAAAATAAAACAAGAAATGTGTGATATTAACGATAATATTTTATTGTTAGATAATATTCAAGATCCAGGTAATTTAGGAACCATTTTAAGAACCTGCTTTGCTTTCAATATTAAACAAGTTATTTTTAATAATTGTGTTGATTTATATAATCCTAAAACAATTAAAGCAAGTATGGGGGCCATTTTTAAAATTAATTTTTTACGTTTTAATGATTCTAAACTAGCATTAAATTTTTTATTAAAAAATAAATTCTATTTAATTGCTACTATTTTAGATAAAAACGCAATTTCTTTAAACGAAATAACGATTAAACAAAAATATTGTTTAATGGTAGGTAATGAAGGTCATGGTTTGGTACAAACTTTTATTGATCATGCTCACATTAAAACAATGATTAAAATGAATCAAACAGAATCATTAAATGTTGCAGTAGCAGCAGCAATTTTTTTATATGAACTTAATAAATAAAGAACTTTTTTTAATTACTGGTGACAATCGTTTAAAAATGGATCAAAAAATTAATGAAATTGCGACCTCTTTTGATGAATTAGTTAAAATTAATGATCAAGAAATTTCATTAATTTCTTTTAAAAATCTTATTGAACAAGATGATTTATTCAATAGTAATAAGATCTACTTATTTAAAAACGTTAACTGATTCGAAAATTTAGAACATTTAAAAAATGTTAGTGATTTAATTGATTATTTTTTTAATAATCATGTTGCTATTATTATTACAATTGAATCTACAAAAATTTCTACAGCAAAAAAAATTCAAGAAACAATTGCAAAATTTAATCATCAAATTACTTTTATGACATATACAAATGAAAATGCTATGGCTTTCTTAAAAGAAGAGTTAAATCAGCGTAATTTATCATTAAATAAATCTATAATGCAAACGATTATTCAAAAAACAAATTTTAATATTAATTTTCTTAATAATGAATTAGATAAAATAGAATTAATTAATGATTTTTTAAAAAATCATGGTATGTTTGATATTAATAATTTTATATGTGATTATGGTGAATATCAAATTTTTAGTTTATTAAATCTTTTGTATCAACATAAAATAAATGAATCAATAAATTTAATTAATAAAATGCTCATTGACAAAATTGATGAACTCACAATAATTAATATGTTAGCGACAATAATGAGTACACATTACTTAATTAAATTATTACATGAAAAAAAATATAACAAAAACGTTATATCTAGCTCTTTAAATCAAAAACCTTATATTATTGATTTAAATTTAAAATTAATTAGAAATTATTCAGCTAAATTTTTACTTAAAAAAATGTATGATTTATTACAATTAGAAATTAAAGTCAAGGAAAATAAAATTGATAAATATTTTGGATTAATTAATTGAGTTTTGAATTTTTAACACTACAATAAGTTCTTATAATTTAAGTTTATTGAAGATAAAGATAAAAAAACTATATTTATATTTGTTAAAATTAATAGACCAAATTAAATAAAATACTTTTGTTTTAATGTCAGTTCAGTTTATTTTATTTTAGATTAATGAGGTGGTTATTAGATGCTAAATGCAGAATTGTCAAGTGCACAAAAAGATTTATATAAATCAATTAAATTATATTTAAACACAATTTTTTTGAATCAAAAACTAAATTATGCTATTAAAAATGATAATGATTATCGAAATCAATTAAATAAAGTAATGAATATTTTTGAATATTATGAAGATAATTATGAAATAGCAAATAAAAATTTTCATAGTTATTGACAGGCTTTTAAGTCATATGTTCATTATGAAAAAATAGTTAATGATTATAACGATTTTGATGAATTAAAAGATATTGTAAATGGTTTAAAAATTTATGTTTTAAATACATTTCAAATAGATTTAGATCAAGAATCACAAAGAATTGCAGATATTGATTTTAAGTATGATGAAAAGACGCAAGCATCAATGGGTTTTTATACTCCAAGAAATCAAGAAAATAATACTAGTACAGAATATGAAAAGGTAAGTGCTGAATCTATTAGTGATGAAGATAGTATGAATCATACTTATCAACCAACTTCTGATTTTACAACAGCAAATATCGATAATTTAACAAATATGGTATTTAGTGCTCGGGTTATGAATGGTGAAATTTATTTATATGAAACGAAACCAAAAGTTATTCCTTTCTTAAAATATGTTTTTTTTGCTGTTAGTTTAGCTTTGGTTTTTTTTACAACATTAACGTATGCTTTTCTAATGGATTTTGGCACACATATGTCAAGTGTTATTAATGTTAATGGTATTACTCATATTGTTCGTTTAGTTTCGCCAACATTTCCAACCCAGTTAGTGATATCAATATTCATTATGATTTATGCTGGAATAAATGCTTTTAAAAGAAAAATTAGTGAAAATGAGAAGTTTCATTTTAAAAATTTTATTTGAATTATTTTGTTTGGAGCATTATTAGTAGTTGCATTAGCAAATATTTTTACTGGAAATTTAAGATTTTCAAGTAGTTCATTTGATGAAATGTTTCGTCAAGCACAAATTAATGATGTATTAGCTAACTTAGCAAGAAATAATAGTTTTAGTTTTGATTATTCGTATTTACAAAGTATTTTTTATGGCTATAGTATCGCCCAAATTTTAGTATTTGTAGTTATTATTTTAGGAACAATTTTAATTATTAGTACTTTTATTTTAAATCCTAAACGTGATATTCAAAGAATAAAATTATTATTACAAGAAATTCATGATGATATTTTAACAGGTAAAATTGATCCTAATACATATTTAAGAACAAAAAATCCCTTTAGAGACTTGTTTGGTTTTTAGATGAATTCAAGATTTAAATTTAACTATGATATAAATAATAAATTAGCATTATCCTTTACAAAGGATTTAATTGCTAATAAAAAGCAAAAAAATAAAAAAAAATTTTTGCTTTTTTTATTTCCCTTTTATCCTTTAAAACTTTTTAAAACGATTAATTTAATCGTGGTTTTTGCTGTTTTAGTTGCAATTCGTTTAGGAATGGGATTTTTACATATTAAAATTCCTGGTTTTAATATGGTTATTTCTTTTACATGAATGGCATTAATGGTTTTAGGGTGGTATTTTGGACCATTAATTGGAATATTTGCTGGATTTTTAGTTGATACTTTAGCATGATTAATTAGTGGTGGAATTTGGTTTTGAATGTATGCTATTCAAGAACCTATTGTAGGCTTTATTGCTGGATTTCTAGGATCTGTAGCTACATTAAGAAAAAAAACTAAACGACCAATAATTGATCTAATAGTTAATCAAATCTTTATTTTATTTTTTAGTATATTAACATTGTTTATGATTTTGACCTTAGTTAATGATGGTAAAAACTCCTTATTTAGCATTCTTAAAAAACGTGATAAAGAAATTAATGAAGATTGAATTAGTATTTTTAAATATTTAGCAATCGCTTTTTTAGCTACATTTGTTTTGCTTGTTGAAGCTTTTGTTATCATTTATTATTTACGTAATAGAAATCGTTCAAAAGTACAGTTAATTAATTTTATTTATTGCTCATTATTAGTAATTTTTTCAACAATGTTATTTTCGTTTGCACTAGGACCTTACGTAAGTGTAGCGTATTTAAAATATATAAAAGTCCCAATTACGAATTATTTAAAATACGGAGTTAATTACTATTTATTACCACGCATTATTAAAGAAAGCATTAAAACACCAATTTATATCATTTTATTATCTTCAATAATTAGTGCATTTGATTATGTCTTTCTAAATATTAAGCAAATTAATATGCACAAATGAAAACATTAACAATACACTTTTGAAGGTATAAAATATAATCATAAGTTTTAAAAGAGAGATAAATGATAGTAGTTTATGGATTCTAAAAAATTTAATGATTTAGATTTAGATATTTTTAAAAAGGGGATTATAAACCTATAAGTTCAGAATTTTTAGCTGAATCATTTAATGCTATGATTCCTATTATGGAAATTCAAGAAAAATATAAATAATTTGATAACGATACTTTTTTAAACGAATTTAAAGGTGGTTTTGTAGGCGTTTATTACCGATTAGATGCTAAAAAGCAAGGATTTAACCAATGATTAGAAGTTAAACAAGTATCATTTAATACTAAAACATGAGGTGCAATTTTAATGATACAAGTTTAGAAAAAGCTAAAGCATTTAGTGATATAAAAACCTTTTTAGCAGTAGGCGTTTGAGATAAAATGGCTGATTTATTATTCATTGTTTATGGTTAAAATCCAGAAATTGGCGAATATTTAACTAAAAAAGTTTGCGAAAGTAAACAAGCTAGTCGTCGATTCACACAAACAATAAGTGTGCGACAACTAGTAAAAATTATAAATTTAAAATTTTTCCAGTAAATTAATAATAATCCTAATTTAATAAATATTTTAAAACAAGCAAAACTACCTTGAATTTTAGAAAGTTTAACTATACTACGTTTAGAAAAACTAAAAAAAACATTTCACATTTACTTATGATACTATTGATAATGTTAAAAAATAAAGTTTATTTAATTTTTTTAATATGGTAAAAATCATTTTTATTAGTTCGTTTAGTGTAAGAAATAATTGTATTTGTGTATTTATCAACTAAAAAATAATAACCTTTATTTTTAGGGTCCATGTAAATATGGAAATTATTGTCTAAAGAATTTAAAGGACGAATTCCTAAAATTCGCTCATTAATAATTGGAATAATGAATGTACCACTTAGATTTTTAATGTTAACATCAGTTCATTCAGCACGTTCTCGAAAACGAGCTAATGCATGATCTGTAATTCTAAATTTGCTTTTATAATAATATGACATAATTCAAAATTAAATTAGTTATGATATTTTAATGTATATTATTTATTCATTTATGAATTAAATGTAATGCAACACGATTGTGTTCATTTCATAGCACACTAACATTAGCATTCTTCTTACTTGGTTCAACAAATTGATCATACATAGGGCTAACAGTGCTACGTCATTGAGTAATTACAGATTCAAATGATCGATTACGTTCATTAACATCTCGAAGAATTCGTCGAATTAAACATTCATCTTTTGGAGTTTCAATAAAAATTTTCAAATCAGCTATTTGATTAATGACATCATCATAAATTGCATAAATACCTTCAAGAACTATAACATCAACATCACTAATATCAATCGTTTTATCTAAACGAATCTCAGTTTTATAATCATAGATTGGTACTTTAATTTTTTTACGTTTTTTAATGTCACTTAATTGCTCTCGCATTAACTCTCATTCAAAACTACTTGGATGATCATAATTAATTAAGCGGCGTTCTTTTTTATTTAGTTGTGAATTAGAAATATAGTAAGAATCTTGACAAATAACAACACTTGTTGTATTTTTAGGAATTCTTGCAATAATTTCATTAGCAAAGGTTGTTTTTCCAGAACCAGAAGCTCCTGCAATTAGTATAAGAATTGGGGATTTAGTATTCATAATTTTGAACCTTAATATCATTAAACATATTACTATTATTATAGATTATGCGTAGCTTTTCCTTTTAATTAATTAAATATTTTTTTAAAAAAAGATATTAAAAAATAATAAAATCATATATAATTTATAAATGTTATAAAATTCAATATCTTATAAGATACACAAACTAGAAATGAGGCGATAATATGGCTGTACAACAAAGAAGAGTAAGTAAATCAAGAAAAGGAATGCGTCGTAGTCACGATCACCTTACAATTTCTAATACAGTAGCATGTAATGAATGTGGTAAAGCATTACTACCTCATAGAGCTTGTCGTGATTGCAAAACTTACAGAAGTATTAAGTTATCAATCAAATAATTTTTATGATGAATGATAGTGTTAAACAAACCAATAATATTGATGAAAATCAATTAAAACCAACTACAAAAAGCTTTTTTGATGATAAAAACAACCAATTAACAAAAGTTTTAAATATTCCTAGCGTTGTTAAATATACGGAATCAAAAATTAAAAAAAATGGTAAAAATTTTTCAATTAATTTAATCATTACAATTACAACAATGTTTATTGTAATTGGGATTATTATAACTATTGCTATTGCTATTGGTATTAAATTAAGTTAGTAACAACTTCATGAGTTGTTATTTTTTTATTTTTTTTAGATATAATTTAATTTAGACGTATGCTATTAATGTATAATTTTAAATAATGTTTTATATTAATTTAAGGGTGAAAACAATGATGGAAAAATTAAAAAACAACAAGACTTTGTTTTTGAATGTAGAACAAGCTCAAAAACTAGGGATTAATAACCCTTTAACACAAGAGATTTTTTTAGATGATCAAAAGTTAAAAGTTCATTTAATTAATAGTATAAAAGATGAGTTTTACTTAAAAAAAAGTATTAGTAAAGGTTATGCTTATTTTAATAGTTTAGAACAATTTAAGACTATGGAGCTTGATGTTTTAGATAACTATCAATTTGCAAAAATAGCAGCTACTAATTTAGATGAGTATGATCAATTAGTTTACACAAAAACTCGTCAATATTTAATTAATAAAGAATTTTGAACTAATGATGGTTTAGATGCATTTATTGGTGATGATTTTAAAAGAAAACAATTAACGAGCAAAGAAATCGTTAATCTAAAAACAAAATATGATTTATGAATACAAGAATTACATTTTAATAATGCACGAATTAGTAAACAAATTCATAATTTAAGTATTCATGGTCAAAATAAATTAGATTATGAGAAATTTTATGATAAAAAAGCAACCCTTGAATCAAATTTAGATATTCTTCAAATTTTAATTTATTTAAGATCTTCATTACTTGAGTTAGATTTTGACGATCTTTTTAAAGATGATCAAAATAAAATAAGTATTCGTCAATTATTAGTTAATGATTTAGAATACTTGCCGTTTTCATTTCAACGTTATGATTATGAATCAAGACGTAATTTCATTAGAAACTCACGTCAATTAGTTGATACCTCAATTGATTTTGACGATCTTGAATATGATTTTTTGCACAATAAGAAACCAAAACAAGAACTAGAAAAAAATAATGAATCAATTAAAATAGCTGAATTAAAAAAATTTGAAAATTCACAATTAAGTGATGAACAACAATCTAATTTAGAAAAAGAGTTCTATAGTATTGGAAGAATTAACTGTTTTGACGAAGTTAGCGAAAATAAACAAGAATTAAACAAAGAACAATTAGTAATCGATAAAAAAAATGGGTATTTGAAGCCACGTAGTTACTTACGTCATCATGAAACTCCATTTATGGTTGACATTGAGAACCGGGTAGACTTTTTAATGGCAGGATATGAAATTATTAAAAATGATGATCCATATGCAATTAAACAAGAAATATTAAAAAATACCATTTTAGAACAACAAAAGCTTGTCGAACAAGATGATCATGATTTTAAAGTTAAAAATGATGATCAAGAAGCAAATGAAGAATTATTGGACAATACTGATAAATTAGAAAATCAAGATTCTACAAGTGCTGTTGAATTTAATGATTCATCTGAAGTTACTCAAGTAGTAAAAACGGATGAAAATGTTGATAATTTAATTAAAACTAAGCAAATAACTAGTTTTAACGATCTTAAACAAGAGCAATCATCTCAAGAAAATATTATTAAAAATGATCTTTCATCAATTCAAATTCGTTTAGATGAAATTATTAATGTACATGAAAAAGATACTAAATTACAAACCGAACTAAATTCAATTATGATAGATAGTAATGAAAAAAATATTGATGATAGTTTAAATAATTTAAATGAACAAATACGAATTCAAGATCAAGAATTAGAATCATCAGATAATCAAAATGTAATTATTGAAGAAGAAATTAATACCAAAAATTTAAATAATAAAGCTGATGTTGGTGTTATGAGTGTTGAAAAAAAATATGAACCTGAAGAGGAAATGGTTAATTTTGAATTTGAAATACCTGAAAACCAAAAAGAATCAGTTAACCAAGATGTTTTAAATAGTAATAGTATTGATAATTTTAAAGAAGAAAAAGCTAAAGAAAATACTTTCTTAACACCAAAAGAAATAGAAAATAATGATTTATCATCACAAATTGAATTAACACCTGCTGATTTTGAACTTAAGACACAAGAAATTAATGACGAAACTGAACGTTTATTGGATGAATTAAATAATAATAAACCTAAAGAAAAGAAGAAATTTTGGACATGATTTAACTCAAAAAAAAGATAAATAAAAAAAAGGAATTATTAATTATGAATAAAACTAATCAAATTGTGGATTTATTAACCAATAAACAAAATAATTCAATTCAAAGACGCGATAATCTAGTTTTTGTTAAAAATGAACAAGAATTCAAAGATATTTTAGATTCTACTATTAGCTTAAGTAATGTTTATGATCCTTTGTTAGGTAATGGTTGTGAACAAGAAAAAATAAAAGAATTAACTATTTTAAATAATAGTGTTGATATTAGCAAAATTGAAGAGTATACTAAAACATTAGAAAGTAAAGTAGATGATTTATACAATAATGTTTCTAACCAACTAATTCAAGATTATCAAAAAAATGAGAATACAAGTGCGAATAATATAATTATGAAAAATGAAGAATTATTAAATTCTCATTTAAAAGAAGTCGAAAAAATTTGTGAACAGCTAATTGATGAATGAAAAATTGAAATTTTAGGGGAAGTTAATTTTAAATTTCAACAACAATTAATAGAAATCGATCAAAAACAAGAACATAAATTGAAAGTAAAATCATTAATCAATGAAGATAGTTTTTTAACAAAAAGACAAGTTAAAAACAAAAATAAAATTCTTACTGAATTTGAAAAACATCGTTCACAAATTACACAAAATTATTTAAATAAACGTAGTGATATTCTCAAGAAAGAATATGATGAATTATTAAAAGATGATAAATTATTTGAGTTAAGCTCAGGCGATAATTGAAACAATTTTAAAAAAATAACTTTATTTATTTCTAACGAGATCGGAATCATTAATGATTTAGAAGATCTATTAAACCATCAAGAATTAATTCGGACTCGAAATTATACAAAACGTGCTATTTTAAATCAACATCATATTAATGGTCACTTTAAAGATGAAAAAAAATATTTGAAATCTTTGTATGATTTACGTGTTAAATTTATGTATGGTTTAGGTTCAGAAATTGTTATTTTAAAATTTTTAATTGAGCATTATAAAAAATTAAATAGTTTACGTTTAAGATTAGAAACATTAAACCACCCTCTTTCAAATTATGCTGTTTATTTTGATATTTTAAACCGAATTGAAAAACAAACACAATTGTTAGCAAAAATTGAAACTTTAAAATATGATAATTATGATTTTAATGCTGATGTTATTTTAGATGATTTAGAAAGTAATATCACAAAAATAAATGAGATTAAACATAATTCTAATGTTTTAAATAAAACAAAAGTTATTAAAACGATTATTGAGCCAAAATATGAATTAGAATTAATTTCAAAAGATGATAAAGTTGATGGTGTTAAGGTTTACGTTAACGATCAAGAAATTCTTTCTAAATAATTATTTTAAAGGAAACACAAAAAAGGCTTTTAATAATAAAAGCCTTTTTATTTCAAAATGAAACTAAAAATTGATTTTTTATACGAAAAAGTCTTAACAGAATTATTAACTAAAAAAAACAAAACTAAAAAACGTATTTATGTTTTTTATGATTCAGATTTAGTAAATTTTAATAGTGCATATATTCAATCAAAATTAAAATATGCTAAATTATTTTCTGTTAAACTTATTATTTATGATTTAAATGCATACCAAATTCAAGAAAATCATCATTTTTTATCAAAACTTAAACAAGCAAATAAGGATTTTTTGTTTTTTGAATTCCCTTTGTCAAAAAAACATTTGGATCAAAATTATTTTCAATATTTAACTTTTCAAAATGATCTCGATGGAATTAATTCACATTTGTTTTTAAATAAAAAACAAGAAAATCAAATTATTTACCATCCAGCAACAATTAATGCAATTTTAACATTAATTAAAGAATTAGATGTAAATTTAAATGACTATCGAATTTGCATTATTGGTCGTTCACCCTATTTAGGGGATTATTTATATAAGTTATTAAATCCTAAAAATAATAAAATTAATATTATTCATTCACAAACTAAAAATCCATTAAGAATTATTAAAGAATCTAATTTTATTATTAGCTGTGTGAATAAAGCACACATTTTTAATTCTAATGCATTAAGTGATAATTCTTATTTAATTGATGTTACTACAGAATACATCAATAATAAGTTATGTGGTTCTTTTTTAATTAATAAAAATGATGCATTAAATCGAAACATTAAATACACTCCTGTACCAGGTGGAATCGGTAAATTAACGACGTTATTTTTATTTTTGAATTATTTTAAATAAGTGTTACTTATCAGCCATATTTTTTTGTAGTATTATATAATAATATAAATAATCAAATAAGAAAATAGTATTTATTATGATTGACAAGCAAAAAATTAAACAACTAATCCAAAATCCTGTTTATGGATTTTTTGATGAATTTAAAAAAAATTTAAACAGCGATCTTGATCATGTAATTTTAGAACAATTAAAAGATCAAGATAATAATATTGATAAAATTAATCATTTAGTAACCGATATTAATAATTTAAAAAATGATATTAAACAACGCAAAAAAGATGAAAAAATTAAACAACGGACTTTTTGTAATATTCTAATCGCAGTTTGTTTTGTTATTATTATTGGTTTGTTTTTTTTACATTTTTATTTGAAAAATTCCAAATTTATTAAAAATTTTAAAAAGTATGAAATTGAAAAAAATAATCAAATTAATATATGTAACAGCCAAAAAAATAAATATATTTATAATGTTTTATCACAAATTAATTCTTATAAAATTTCGCAAGAAATTTTTGCTAAGAATGGTTTATATTTAATGCCAGAAATTCAATATGAAAATATTAAACAAACCGATTTATTAATTTCACGAAACGCAAAATTTGTTGGATTTTTTGGTGGTTTAGAAGTTAAATTTAAAAGTACGGATACTTTTTTAGTGTATTATAAGGAATTTTCAATTAAAGATGTTGTAACAAGTGGTTGTATTCAAATTTCTTATCGAAAAGGAGATCAAATGGTTAGTGAAACGATCGTTGCTCATCATAAGGAACCAACACCGTTTGTTGATCTTCACAGTAATTTTGTTCATAAAACTAATTACTCTCCAAACTTTAACTTTTCAACGTACGCTCAAACTGTTAATTCTCGTTCAAAAACAATTTTTTCTAATTTAGAATTTTCTAAGTATTATGGCTTAGGAATTGCTACTCAAAATTTAGAATTTGATACAAAAATGCTAGAATTTTTTACGCCTTTTAGTCAAGAAAACTATGAAAATTTTGCTAAATATTTAAAACAAGATGATATCACAGTTCCTATGTTTACAAAAACTGCGACATCCCTTATTATTGATGATCAATTATTAAAAGATAATAGAAAACCATTCTTTATAAATTTATTAACAAATAATAGTTATGTAAAAACTTGTGATTTTCTAATTGATACAGATATTAATGCGGACCTACAAACGAATTTAAATATTTTAAAATATGAATTAGTAAAATTATTTGAAAAATATATTTTATATTTAACTACAACAAGTTTATCAAGTGTAATTGCCCGAGAATGATATATTAATAATGATACTTATAAAATTGGTGATAATTACGATTATGAAAGTTATTATTATACTAATCAAAATCAATTAACTCCACTATCAGTTTCGACAAAACTTTTTTTAAAAAACCAAATAGCTTTTGTTAATGATTGTGAAGTAATTCCTTTTGCTGAACTAGTTAATAATCAAACACGATTCGGCATTAATAAAGCACAGTTTAATATTAAAAGTTATCATGTTTATAATCGTGTAGATAATGTACCAGTATATGCGCATGGCAGAACACACATTGTTCCTGTACAATACAAAGAGTACATCGAATATGAATATCCATTTATTTGTTTATATGTTTTAAAATATAAACAGTTAACAGAAGAAATAGGTTTAAAATATTTTACACGCCCATTTAATAATATTTTATTAACAAATTTTGAAGATCATAGTGCTTGTTCTATTGATGAAATTCTTGATTTTAATGATAATGAATCCAAAAATAATAGCGATAATGATTTTAAAATTTATTTAAAAGAATTAGATACAATCGTCACAAATGCGAATTTAAATAAAAATGAATTTAGCTTCTTAAAAGACAATGATGGTTATTTCATTACGATAAGTAATAAAATAGAATTAGACGAAGAAACTGAACAAGAATTATCAACTTGATTAAGAAAAGTAAGCTTATAGAAAACAAAAATAAAAAATAATTTGCATTAAAAATTAAACTCAATCAAATTATTTTTAACAAACGAAAATTTATTTTTATAAAATTAGCACTCAATATTGACAAGTGCCAAAAAATAATGTATAATATCTTATACTTTCAAATATGGTATATGTTTAAAAGGAGAATGTGATATTTATGGCAAAAGAAATTATTTTAGGAATTGATTTGGGTACAACAAATTCATGTGTAGCCGTAATTGAAAATAAAAAACCAATTGTTTTAGAGAATCCAGAAGGAAAACGAACAGTTCCATCTGTAGTTTCATTTAACGGCGATGAAGTTTTAGTAGGTGATGCTGCAAAACGTAAACAAATAACTAATCCAAATACTATTTCATCAATCAAACGTTTAATGGGTACAAAAGAAAAAGTAACTGTTTTAAATAAAGATTATACTCCAGAAGAGATTTCAGCAAAAATTTTAACTTATATTAAAGAGTATGCAGAAAAGAAAATTGGAGCAAAAGTTAATAAGGCTGTAATTACAGTTCCAGCATATTTTGATGATGCACAACGTCAAGCTACAAAAAATGCAGGAATTATTGCAGGATTGAGCGTTGAAAGAATTATTAATGAACCAACAGCTGCAGCTTTAGCTTATGGAATTGATAAATTAGATAAAGAACAAAAAATATTGGTATTTGATTTAGGTGGTGGTACATTTGATGTATCAGTTTTAGATATGGCAGATGGTACATTTGAAGTATTATCAACAAGTGGTGATAACCATTTAGGTGGTGATGATTGAGATCAAGTTATTATTAATTGATTATTAAAATCTATTGCTGATGAATTCAATATTGATTTAAGTAAAAACAAAATGGCTATGCAACGTTTAAAAGATGCTGCTGAAAAAGCTAAAATTGAATTATCAGGTATAAACACAACAACTATTTCATTACCTTTTATTGCAATGGATAGTTCTGGTCAGCCAATTAATTTTGAAAAAGAATTAAATCGAGCGACATTTGATAATCTAACTAAAAATTTAATTGAAAGATTAAAAAAACCTGTTTTAGATGCAATGAAAGAATCTAAATTATCATTGGTTGATATTGATCAAGTTTTGATGGTAGGTGGTTCAACACGTATGCCTGCAGTACAAAATTTAGTGAAAGAATTAACAGGTAAAGAACCAAACCATTCACTAAATCCTGATGAAGTTGTTGCAATTGGTGCAGCTATTCAAGGTGGAGTATTAGCAGGAGAAATTGATGATATTCTATTACTTGATGTAACACCACTTACTTTATCAATTGAAACAATGGGAGGTGTAGCAACTCCTTTAATTCCAAGAAACACAAAGATTCCAGTAAGTAAATCACAAATTTTTTCAACAGCTGCTGATAATCAACCAAGTGTTGATATAAGAATTGTGCAAGGTGAACGTTCATTAGCTGCTGATAATAAATTATTAGGTAATTTTGAATTAAGCGGAATTGAACCAGCTCCACGTGGTGTTCCACAAATTGAAATTAAGTTTAATATTGATGCTAATGGGATCATGAGTGTTAACGCTAAAGATTTAAAAACACAAAAAGAAACATCAATCACAATTAAAGATTCACAAGGATTAAGTCAAGATGAAATTGACAAAATGATTAAGGAAGCTGAGGAAAACAAAGAAAAAGATGCTAAAGTAAAACATGAACGTGAACTTGTAAATCGTGCTGATAGTCTAATTAACCAATTAGAACAAGTGTCAAAAACTGAAAATGTTCCACAAGAACAAAAAGATGTATTTAACAAACAAATTGAAGATTTAACAAACGCACGCGATGCTCAAGATTATGTAAAACTAGAAGCTGAAGTTAAAAAAGTTGAAGATTTATTAACTAATGCTGCTAAATTTGCTCAACAAGCTCAACAGCAAAATCCAGATAATCAAAACAATAATAAAGATGATGTTACTGAAGCAACTGTAACTGATGATTCAACAAAGAAATAATTAATTTTAATAACCAATGGTTTAAATACCATTGGTTATTGTTATATAATAATGTATAAGTTAATATTTAATTTATGTATATATATTAAAAATAAAGGAAAATTTGAGTATATGGTAGAAAATAAATTTTTAAAGCAAATTAAAGAGGGAGAGCAAATTAAAATTGAAATTAAAGGTTTGGATAAATTTAATAATTTTGACGAACAAAATTATGGTTTAAATAATATTTCACCTCAAATTCGTTGATCACCAGTACTTGAAACAGCAAGTTATGCGGTTGCAATTATTGATTATGAAGCAGTAGGAGGCGCTCCATTTGTGCATTGATATGCCTTAAACATTTTTGAACCCGAATTAGCATTAAATGTTGCAAATCTTGGTTATGCTAATTTATATCAAGGTGAAAATTCAACTTCAAAACATTTTTCACATTCAATATTAGATGCAAATTATCAAACTGATATAGCAAATAATTATTTCCCTCCTTGCCCGCCAAATAAATCACATAAATATGAAATTAAAGTTTATGCAGTGCATCATAAGCTATCAACAAACCGAAACAATATTTTATATTTAGACGATTTTGAAGCGAAATTAAATGAAGCAAAAATTATTGCCACAGGTTCTACTTATGTTTGTGCTCCACAGATTAGTTTTGAAGATGATAAATTAATTATTGGTTCATTAGAGCATAAACATCCTTATTTGTTTGCTAATTGTGAAAATGAATATCATGTAATAGAAGATGTAATTGTTACAGATATTAATAAGAATATTTATAGTGAAAATCAACTAGATAATTTTAAAGATCTAGAACAAGCAAAAATTAAAATTATTGATTCTAGTGATGCTTTATCGTATGCAGTTATAATTACTTCAAATCATCTATTTAAAAAATTTGGTCGTCCAGTTGTTAATTATGCACACGTTATCGAGAAAAAAACTAATAAACGAATTAAGTTCAATAATTCCTACAAATTAAATCATGCCTTTAGCGAAATTAGTCCTTCAAAATATGATCATTTAGGTAATGAATTATTGATTTTAGAAGATAAAGATGCTAATTTTGAAAGTGGACTATTCACAATTCATGTGTTTGGATTAAATAAAAAAATTAAGTATTTAAATAAGATTGATAGTGTTGCAAATTCAATTAGTGATTTATTTGAAATGATTAGCGGTAGTGTTATTTCTTATAAAGCGAAGTTATTTAAGTAATATTTTTAAAAGAATAATGAAATATTAAATTATTTAATGATAGATCGTAAACAAATTCATGTTAAAACTAGATTAACAATTAATGAGTTAAATAAGTTTTATACTTTAATTAGAGTCCAAAAACAAAGCTTAAAAGAGATTGAAGATAAAATCAAAAAACTTGATTGAATTAACTTTAGTGAGATTAAAAATCAAAATTTATTTGATGAATTTAAATTTGATTATAAAAATATGACACCATATGTTATTTCACAAGTTCAAGATCTGGTTAGTGTTTATGATGTAAGACAAATTAGTGAAATAATTCTTGAATTAAATCAAGTTAATATTCAAATTCAAACTAAAGATAGTGAGTTTTATATTAAAAATAATAAAAAATTAAGTTTAATTAGTAGTGAATTTGAAGATTATCAATTAAAAAATTATGATGCATATGAACATTTTAAAATTACAATGGATGAACCTAAAATTCATACTTTAAATCATCAAATGCCATTTGATTCAAAATTAAACATCAAAACCATCATTTTTATAGTTTTAACTTCTTGTTTATTATGTTTATTAATCGTCATAATTATATGTGCATTATTAATTGCAGGAGTTATTAATTAATGAAAAAATATATCAGTATCGCTATTGATGGACCTTCAGGTTCTGGAAAAAGTACAGCAGCAAAAGAATTAGCCAAGAAATTGGGATTTTTATATATTAACACTGGTTTAATGTATCGTGCTTATGCTTATTTTTTATCTATTAATAAATTAGATATTAATGTTAACGAAATGGATTGTATTAATGCCATTAAAAATGCTAATTTTATTTTTAATGGTGATAATGTGAAAATTAATGACAACGATGTTAGTCTTATCTTAAGAAGTAATGATGTGGCAATGATTGCTAGTGTTGTATCTGCTAATGAAAAAATTCGAGATTTAGCAACTAATGAACAACGCAGAATTGCTAGTGAAAACAACGTTGTTATGGATGGGCGCGATATTGGAAGTATTGTTTTAACTAATGCTGATCTTAAATTTTATTTAAATACGAGCATTCAAACACGCGCTAAACGACGTTTGTTTCAGAATAAAGATATTGAAAATTTGGATTATGAATCAATATATAACGATATTAAAGAACGTGATCATCGTGATATGACTCGCAAAATTGCTCCACTTAAAAAAGCAAACGATGCTGTTGAAATTTTTAACGATAACATGAAATTAGATGAATGTGTACAACATTTGTATGAAATTTATTTAAAAAAAATAAAAAAGCTAAATTTTTAATAAAAAAATAACAAACAAAATTAAAACTTTCATTTTTCTATAAATCTCATAATTTATTAATGAGGTGTATATATTTATGAATTTTGATATTAATTTAATTAATGAAAATGATTATGTTTTTAGTGGATACATTATTTTAAAAAAACACAATGAAGAAATTATCGAATTACTAACTTATTTAGGCGATGATCCTATTAATGATGAAGCCAAAATCATAACTTTATATAATAATGATCATATTGATCTAAAAAATCTTAATGATGGAGATTTAATTATTGTAAAAGCAAGTTTAATTATAAATAATAAAAATAACAAACAATTATTGCTTAAAGATATTAATAAACTAGAGTTAAGTATTAACTAATTATTTTCAAATAATTAGTTAATCATATTTATAATAATTAATGGTGGTATAATGAATTTTTTAAAAACCTTACTGTGATTTGTTGCATATCCTTTTGTAGCATTATACATAATAATTCAAATGATTTTAGCGGCTATGATGGCAAATAAATATAAAAATGATCCAGAGTCTGTTAGCGTTAATGAGCGCTATAAAAAAATAATGGGTATTATTGATGCTATTTTATATTTAAAAAATATTAAAGTAGATTTGATTGGTAAAGAAAATGTTAGTATAAAAAGCATGTTATACATAGGTAATCACAAATCTAATGGTGATCCAATTGTTTTATTAAAGGCTCTTTATCAAATTAGCCCTCATAATGTGCGTTTTGTTGCTAAAATTGAATTAAAAAAATCATTTTTAAAATTTTTATTTAATTTAATTGATGTAATTTACATTGACCGTAATAATTTAAGACAATCAATTTCAACAATTAATGAACAAGTTAATGCTTTAAAACAAGATTATCGTGGATTAGTTATTTTTCCAGAAGGAACACGAAATTTTAATCATGAATTTAATGAGTTTAAATCAGGCGCTTTAACAGCTGCTTATAATACATTATCTTCGATTCAACCTTTTGTGATTTTTAACTCACAAGGTTCGTTTGAAATGAAAAACGATCAAAAGCAAAAGTTAAGATCAAATCCTTTTAAAACCCAACATATTAAAGTTAGTTTTTTAGAACCAATTCATTCTTTTAATTACATTAATATTAATAAAGATATTTTAATGCGTAAGATTCAATCAAACATTAAAAAAAATTATGATGAATTGTTAAAATCAACAATTAAAACAAATTAATTTTCATTAACAGTAAATACTTTAAATAAATTAAAAAAAGTTTATTGAATTTATTGTTTTTTTATACTAAAAGTATTTTTAAAACTAATTTTTTTTATAATTAATTTATAAGTTTAATATAATAATTTTTGGTGAATAGAATGAGTGAAAAGCAAATCCAAAATAGTTTATTTGAATATAAGTTTATTGATTTTAACGGTCCATTAGATACATTGTGTGTTTTAATTAAACAAAAACGTTTAGATATTAATAATTTAGATATTTTAGAGTTGTCAAAACAATATGTTAATTTTGTAAATCAATTAATTAAAACGATCGATATTGATATTTTAGGTGACCATTTAGCAATGGCTTCTTATTTACTAGAACTTAAGACTCGAATGTTGATGCCAACTGTTGATGAAAAACAAATTATGAGTATTGAAGAAGATCGCCAAAATTTAATTGATCGTTTAATTGAATACAATGGTTATAAAAATTTAAGCGAACATTTAAAACAACGTTTTGAATTTAGAGCAACTATGATGGATTTACCACAACAAGATTATGAGCAATTTTATTTAAAAGATGCAATTTATAAGCCTTTGCCAAATCATTTAGACTCCATGATCTTAAAAAATATTATGGATAAAATCATTTATGAAAATGAATTAAAAAATTATAAAATTAATAAGATTAAAGTTCATGAATATGATGTTAAACAATTAGAACAATTGTTATTAAATTATTTAAAACAATCGCCTAATCAACAAGCAAGTATGCTTAGTTTTTTTGATATACAAGCAGTGATTGATAAAAATAAACGTTTTTTTGCAATAATGTTTTTAATTATTTTAATTTTAATTAATCGTAATGAAATTTTATTTGAAGAATTAGATAATGATTATTTATTAAAAATTAATATTGAAAGAGTTGTTGATGATTATAATTCTAGTAGTGTAGAACAAGCTAAAAATTTAACAAGTAATTTAACAAAAGATACAATTATTAATTTTAAAGGTGAAGACAATGAATAATGATAAAAAAAATAAAAAGAATTTATTAAATAATGAATTAGATTTAGCTAAATATACATTACATAATCTATCCGCAGATAACAATGATGATGAAAATGAATTAGAATTAGATTGAGATGGTATTGATGATGAAATTATTGATAACAATCAACAAATTTATCAAAAAAAACACATTAATCATAAAACTCGTTTATTTATAGAAAAAGATGATAAGTTAAATAATCATGAACAAATTGAATCAGGCAACGAAATTATTAATCAACCAAAAGCATTATCAGGTCAAGATTTCTTAGAGTTTGTTAATTCTAAATTAGTACAAAAAAATAATAATTTTAAGCAAAATTCTAAATTCTTGCAGAATCAATTAACCAATCATGAATTATTACGTGAAAAATTAAAACCACAAGAAGATTTTAACAAGATATTTAAAACAAAAGATATTAATGTTACTGAAGAAATTAAAATTAAGGATGTTAAAAAAAATAGTAGTGTTTCTTTTCGAAATTCATTTAATATAAATGATTTGAATCAAAATCAAATTACAAACATTAAATCAATTATTGATTCTACACTGTTTTTAGCTGGTGAAGAAGGTGTTTCTTTGCAAGATTTAAAAAGAACAACAGGATTAAATGAGAGTTCTCAACTTAAAACAATTTTAAATCAATTACAAAAAGACTATGACGTTGATGATTCGGGTTTAGTTTTAGTACAATTTGGTGAAAAATTTAAATTATTAACTCAATCCAAAAATAAAGATGCTTTATCAAAATTTGTTACAACAAGTTTTAGAACTCCCTTAAGTCAAAGAAATTTAGAAACGCTTGCTATTATTGCTTATAATCAACCAACAACTCGTGCAAAAATTCAAGCAATTCGTGATCGTGATCCCAAACCTGCCATTGACGCTCTTTTAAAATTGAATTTGATTATAGAAGCAGGCCGTCAAGACACACCAGGTCACCCAATTTTATATACTGTTAGTCAAAAATTTTATGATTTATTTGGTATAAGAAATTTAACAGAATTACCAAGATTAAATAAAGAAATAAATGAATTTAGTCCTATTGATGAATCAACTAACTAATAAAAAAATCTTTATAAATGTTAAAATATAAAAATATTATGTAAACTTTATATTAACATACATGATGTAATAAACGTTAATTGGTGGAATATATATGAAATTACTAAATAAAATTAAGAACGAAAATGCGATTGAATTTCAAATTTTAATTGAAAAATCAGAATGAGAGAAAAAACACAAAGAATCATTTGAAAAAATTGCAAAAAAAACAGCTTCAAAATTAAAAATTCCTGGTTTTCGTCCAGGAAAAGTTCCGGTTGAAGAAGCTAAAAAACACGTTAATGAAATTGAAGTTTTTGAAACAACAACAAATGATTTAATACCGCAAGTTTTAACTTTTTTAGAACAAGATGAATCATTTATAAATGATGATTCAGAAACTGTTGACACTCCAAGTGTTGATATATTAGATTTTAAAGATGGAGAACTAAGTTTAAAAGTTGTTTATGATTTATACCCTGTTGCGACAATTGAAAGTTATAACGATTTAATTTTAACACCAATTGTGAATGAAGCTTTTGAACATGAAGTTAATGCTGAAATTGAACATGCATTAAATTCTAAATCACAAAGACGTGTTAAAGATGATAATGAATTCATTGAAAAAGGGGATGAAGTTCGATTTGATTTTAAAGGGATGATTGATAGTGTGCCTTTTGAAGGTGGAAGTGCTAAAGATCATTTATTAACAATTGGATCTAATCAATTTATTCCAGGATTTGAAGATCAAATGATAGGTTTAAAAAAAGATGAACGTAAAAATATTAATGTTAAATTTCCAGATGATTATCATGCAACCGATTTAGCAGGCAAAGCAGCAGTATTTGAAGTTTTTATCAAAGAAATTACAAATGTAAAGCCACAAGAATTAAATGATGAATTTGCTAAATCATTTAATTTGCCTAATGTTAATACAGTTCAAGAATTAAAAGATTATATTCACAATCAAATTGTTTTAGCTAAACAAGAAAAGAATAGTGAACGTGCCTGATTAGAGATTGCTCAACAATTATTAGCTAAAGCAAAGATAACACCAATTCCTCAATCATTAATTGATCGTGAAGTTTCAACATTAAGACAACAAGTTTTATCACAATTATCACAATATAAAATTGAATTAAAACAATATTTGGAATTTAGTAAAAAATCAGAAGCACAATTTCAAGAAGACTTGATAAGACAAGCTAAAGAAACTATAACTTTAGCACTGTTAGTTGATGATATTGCTGAAACACAAAATATTGTTGTATCTGATGAAGAAGTTAAAGAACGTGTTGCGGAAATGGCTAAACTATACCAAGGGGAAGAACAAGCTGTTATTGAACAATTATCAAAAAATCCTGATGCTGTTAAAGAGTTCTTGTTACATAAAAAAGTTGTTAATTATTTAATTGACTTAAACAAAAATAATCAGCCAAAAAATACAACTTTATCTTCAAAATAAAGACAATCAAATAAGAATTAAAGAAAATTTAGGACAAAAAGGCAAAATCAATGTATAAAATTAGCACTCGTAATAATAGTTTGCTAATTTTTATTTGTTTTTGTTATAATATCTTAATACAATAAAGATTAGAAAAGAGGTTGTTAATGAAAAAACCTATCTTAATATCACGAGCAATTGTTGTTTTACCTTATGAAACAACAACAATTGAAGTAGGACGCCCTAAATCAATCCAAGCGATTGATTTAGCTAAACAATCTTCAAGTAAAGAAATTATTATTATTTCACAAAAAGATATTGATACTGATGAAGTTGTTAATTTTGATGAATTGTATAAAGTAGGAACGTTAGTTAAAATTAAATCTATTATTGATAATTTTGATGAAGGTTATTCAATTGAAGTTGAAGGATTAAAAGCTGTTTACATTAATAATGAGAATGATGTTATTGATGCTATTGAATATGAATATGAAGATGTCATTACAAACCCTATTTTATCAAATAAAGATGAAATAGCAATCAATGGTATTAATTCTGAAATTTTCAATATAATTAACAAGCGTTCAAGACATAAAAATATCAATTTTGATAATATGCACGCTTTAATTTCACTTGAAAAAGAAAAGTTTGCTTATTTAGCTGCGGCAACTTATATTAATGATTATGATAATGAAATTAGTGAAAAAACTATTGAAGATCGAATTAATATCTTATTGCAACCTAATTTATTATTAGTTCATGAGACAATTTTACATTTATTATTTGATCAATTAGTAGATAAGCGCGTTATTGAAGATGAAGTTGAAAAAACTATTACTGATAAAATTAATAATAATTTTCAAAAACAACAACGTGAGTTCTATTTACGCGAAAAATTAAAAGTTGTTAAAGAACAATTAGGTGAACTAAGTAGTCGTGAAGAAGACGCTGATAAAATTCGTGCAAAAATTGAAGATTTAGAATTACCACCAAATGTTAAAGAACGTGCTTTAGCTGAATTAAATCGTTTTGAGAATGCTATGTCATCAAATGAATCATCAGTTATTAAATCATATCTTGATTGATTATTGGATTTACCATGAACTCAACAGGGTGTTGATAATACTGATTTGATGTCTGTAAGAACTCATTTAGATGATAATCACTATGGTATTGAAAAGGTAAAAGAAAGAATTTTAGAGTATTTAGCATTACGTATGCGTAACCCTAATCTTAAAGGTCCAATCATTTGTTTAGTTGGCCCTCCAGGTGTTGGTAAAACATCACTTGTTACTTCGATTGCACAAGCTTTAAATAAAAAATTTGTTAAAGTTAGTTTAGGTGGTGTGCGAGATGAATCTGAAATTCGAGGTCATCGTAAAACTTATGTTGGAGCTATGCCCGGACGTATTATTAAGGGTATGAAAAAGGCTGGCGTTATCAATCCTTTATTCCTATTAGATGAAATTGATAAAATGACATCTGATCAACGAGGAGATCCTGCTGCCGCAATGCTTGAAGTATTAGATCCTGAACAAAATAAAAATTTTTCAGATAATTACATTGAAGAAGAATATGATTTGAGCAAAGTGATGTTTATGGCAACAGCTAATTATTATCAACAAATTCCTTATGCTTTGATTGATCGTTTAGAAGTAATTGAACTATCGAGTTATACAGCAATTGAAAAACGTGAAATAGCAAAATCACATCTATTAAAAAGAATTTTTATGGATGCTAAATTAAATGAAAATGAACTTATTTTTACTGATGATGCTTTAGATTTTATTATTAATCATTATACTAAAGAAGCTGGAGTACGAGAATTAGATCGTCAATTAGGTCATATTGTACGCAAGTACATTGTTGAAACTTATAAGAATAAAAATAATCAATCACCTTTGAATTTAAAAGTTGATGAAGCCATGATTATTAAATATTTAGGTAAAATTAAGTTTGATTTTAATAAAAAAGAAGAGACAACAATTCCAGGAATTGTTAATGGAATGGCTTATACAGCGGCTGGTGGCGATTTATTGCCTATTGAAGTTAATCATTCAACCAATGGAAAAGGTGGAAATGTAACTATTACTGGAAATCTTGAAAAAACAATGAATGAATCTGTATCTGTTGCTTTAGGTTTTGTTAAAGCAAATGCAGATAAATATGGCATTGATACTAAAAAAGTTTCTTTTAAAGAAATTGATATTCATGTTCATGTTCCAAGCGGAGGAATTCCTAAAGATGGACCGTCTGCTGGAATTGCGATTACAACAGCAATTATTAGTTCATTAAGTCAAAGACCAGTGCGTACAACACTATCAATGACAGGAGAAATTATGCTACGTGGTAATGTTGGAATTATTGGTGGTGTTAAAGAAAAAGTTATTTCAGCATATCGTGCTGGAGTTCGTGAAATTATTTTACCAATTGATGACGAACGTTACTTAGAAGATGTTCCAAAGTATATATTAGATGATATTAAAATTCATTTGGTAAAACACTATGATGAAGTTTATAATATTGTTTTTGGAGAAAAATAAGAAATATTAAAAAATAAACACTGAAGTGTTTATTTTTTAATTATGTTATAATTAAAATTAAAATATGAGCGATGAGGATATTTAGATGTCAACAAAACAAAATGAACCATTATTTGTAAATCTAGAGGATTTAAAACAAAAAGTTAAATTATCATTTACTGAAGAATTCTCATTTTCGTATGCACTTTCTGAACTTGAAGCAGGAATTTTTGAAAGTCGTAATTTAAAAGGTGCAGATGCTGGTGATCCTGAAGAAATTTTATTAAATGTGGTTTTAGATGTTAGTAAGAAAAAACGTTCTCGAAATGAAATTAAATTTAATAAACTTCAAAATTATTTTATTCATATGAATTTACGTGAAGAACACTTTAGTGAAATTATTGATGTTCTAGAAGCTATTGGAATTCGTGTGCCTGATTATGATTTGGTGATGCAATCAAAATCAAAGACAAACACCAAAAAAAAGGATGAATATGGTATTGATGATACTTTAGAAATTAGCACATCAAAAATTGGTTTTTCATCAACAACAACCGAAAAAGTTGATGATGGTATTAAAGCTTATTTAGGAGTACTTGGAGAATCAAAAATGTTACGCTCTGATGAGGAAACTGAATATGCTAAAATGGTTATTAGTGACGATCCATCTCTAGTTAAAATTGGTAAAAATCAACTTTATACATCAAATATGCGTTTAGTTACTTCTATTGCTAAAAAATATTTAAATCGTGGATTAGACTTAGAAGATTTAATTCAAGAAGGGTCATCCGGTTTATTAAAAGCAATTGATAAATTTGATCATGAAAAAGGACATAAATTTAGTACATATGCAACATGATGAATTCGCCAATCAATTACGCGCGCAATTGCTGATCAAGCTCGACAAATTCGAATTCCTGTTCATATGGTTGAAACAATTAACAAATTAACTAAAGCCGAGCGAAATTTAATTCAAGAATTAGGAAGGGATCCAACGGCTGAAGAAATTGCGCAAGCTATGAACAAAGTTTCACAAGCAAAAAATCAAAAAGAACAGTTAATCACTGCACAAAAAGTTGTTGAGATTAAAAAATTAAATGTTGACCCAGTTTCATTAGATAAACAAATTGGTCATGATGAAGAATCACAATTTTCAGATTTCATTTCTGATGATGAAATTATTTCTCCAGAAAAATATACTGAAAAAAAAGCATTAAATGATCAGATTAATGAAATGTTTGAAAAAGTTTTAAACGATAATGAACAACGTGTTATTAAAATGCGGTATGGTTTATTACCCTTCGAACGTCCTTATACACTTGAAGAAGTTGGTGAATATTTAGGAGTTACACGCGAACGTGCACGTCAAATTGAATCAAAAGCAATTCGTAAATTAAAACATCCATCAAAAACCGCAAAACTACGTTCATTTATTGGTGAATCCGAAAACTAGAATTGCTTTTATTGCTGATTTAATTAATGACGCTCAAAATGTTGTCGATGTTGGTAGTGATCACGCATATTTAGCTAAAATCTTATTGGTTAACAATAAAGCACAACATGTTACTAATATAGAAGTTAATCAAGGTCCATTAGAAAACGGAATCAATAATTTGACTAAAAATAATCTTTTAAAACGAACTACTAATATTTTGAATGATGGTTTCAAGGGATTAATTTTGGAACAAACATTTGATTATTGTGTTATTGCAGGGATGGGAGCAACAAGTATAATAGAAATTTTAGAACAGAATAAAAATCAAATTAAAAATTATATTTTACAACCAAATACTCAATCATATAAAATACGTAAATATCTAAATAATCACTCATATCAAATTAAAGTTGAACACATTTTTGTTGAAAAAAAAATTTTTTATGAAATAATCATTTGTTCTAAAACTATTAATGTACCAAAATTAATAGAAAAAGATTATTATATAAGTAGCCAGATGCATAAGGATTCATTGAATTTATATTTTAAATTTTTAAAGAGACGTTATGAATACTTAAAAGCTTTGGATATTTCGCTAGTTAGTGAAAATATAGTTAAAGAATATGAATATATAAAGGAATTTATTAATGAAAAAAATGGATATTAAAGCACAAGATATTTTGAATTTTTTAACTAAAAAATATGACTTATCAAAAGCTGAAGCTTGAGATAAAAATGGTTTATTTTTTGATGAACAACAAACAATTAATAATGTACAAATAGCATTAGATATCACAGATGATGTTGTTAATGATGCAATATTAAATAATGCTAATTTAATTATTAGTCATCATCCATTATTTACAAATCAAGATTCAAATGATGAAGTAAATTATTTTGTTAATATCGATTTAATTGAAAAAATTAAAAAAAATAAAATCAGCTTAATTCATTTACATACAGCATTTGATGCATCAGCAAATGGAATGAGCATGCAAATGGCTAAACGTTTAGGCTTATTGAATCTTAAGCAAGATGAACAGAATCCATATCTTGTTGTAGGAGAATTAAAATTAGGTGTTAGTGTTGATTACATTAGTCGGATTATTAAACAAAAATTTTTATCACCTATTATTAAATATAACAACGTTTTTAGACTAGAAACAAATCTTAAAAAAATTGGTATTATAGGAGGTAGTGGTTATAAATTTGCTGATGACGCATTTAATCGTTATCAACTAGATATGTTAATTACAAGTGATTTAAAATATCATAATTGATTAGATGCACAAGCTAAAAAACAAAATATTATTGATATGAACCATTTGTCTGAATCAATTTTTATAGATGTAATTTATGATGAATTAACAAAATTTTATGGAAATGATGCAAATTTAAATAAATCTTTATCAATTATAAAAATTAACTATATTTAATTAAAAAAAGCCTTTAGGGCCTTTTTTAATTAAATTTTTAAATCAAGATATGCAAGTAATTCAATAATTGCTTTTTTACGATATGCATAATATGTTGAATAACTATAAGGTAATTCACAAATTCTTTTATTTTGAATATAAATTTCTTTAATAATTTGAGCACTAGTTTTATCTAATGCTTCTAGAACTTGCTCAACATATTCAACAAAATGCTCCCATTTTAAATATTTTTGATCATCTTTTGGATCATAATCTTTTAATTGACGATATAGTTCTCGATTTTTGCATTGAAATTTAGCAAATAAATATTCTTTAAAAAATTTTTTTACCTTAGCCATATCTATTTTACTAATACACATAAAACCTCCATCTTTTTTAATAAAAATAAAGCAAGTATAATTAAATTAAAATATATTTTTTCAGTTTAAGAATATCAAAAATGTACAGAAAATATTTATTTTTTTATATAAAATTTTAGAAATTTTTTCTAAATTTATTTAATATTTGAACTTTAAATATAAAAATAATTTATATTTACATGGAACAAAAAAAGATGATAGCATTTTAAATGTGAAATCATCTTTTTTAGTAAATGTTAATATCTTGAGTTTGTTGACTTTGATCAATTTCTTTATTATTAATGTTAATTAAATTAGTTTTTTTATTTAAACAACCATTTTGGTATTTTGATTTTAGTGACTCTGCTATTTCTTTTTCTATAATGATTAAATCTAAATTATCAAAATCTTGTTTAATTACTTTTTGTAATTCAAAAATTTTTTCATCACTATCCATATCAATAACCATAATGATGTTTAATTCTGCCAAACCAGAATAAATATAATTCATTATTCTCATTAAATTATAATCAGCATTCATATTAAAAACAAAACGATGGTTATATTCTTTGATCCCTTCAATAAATAATACTTTACGTTTTAGTATAAATTTGAATGAATTAATTAAATTCATCCTTGCGATCAAATAATCTTCTAAGCTCATTTTATTAGTTCTAGGTCTAATTTTTTTAATCATGTATATATATAAACAACTAAAAAATAAAAAAAATAGGACTAATAAAATCAATACAACTACACTAGGACTTGAAAAATAAGCTTTTCAAACTTTTGGAATTAATTCTCATTGATCGACATTTAAGATTGATGATCCACCAGTTAATCCATTAATATTAGAAGGAGATAAATTTTTCATAATAATCTGTCCATCAATAATTAAATCTCCTTTAAATTGACCACGACTTAATGTAATTGATTTGATAACACAGTCAATAATTGGCCTTGCAACACCAGCGAGAATACATACAATTAATGCAACGATACAAAATAAAAAAAAGATTTCGATTAATCATGAACGTAAAGAACGTTTTGCATGTGAAATTTCAGGATTTAATTTATAATTTGTAGTTTGAAATCATCATTCCATGAAATAAAATAGAATTTTACTTTTTTTCTTAATATGAGCATCAAAGCTTTCACGCACAATTTTTAAAAAACGATCATTACTATAAATCGTTCCACTATCATGCAAAAAACGAGTGTCAAAACTGCGCTTTTTACGAAATGATTTATTTACAATTAAAATTGTTTTCAATAAATATTTTCCTTTATCTTAATTAATACTATATAAACTTTATTTATTAATATTAACAAATTATTATTAATAATTTATTCTTATTACTGATAAAATAATAACATTATGAGTGCTATTAGTAAAAATATTATTATCATTAACAAACCAATAAAATGAACTTCTAATGATGTTGTTCAAAAGGTTAAACGGGTTATTGGTGCTAAAAAAGTTGGACATGCTGGAACTTTAGATCCTAATGCTTCAGGTGTTTTAGTATTGGGAATTAATGAAGGAACAAAACTATTAACAAAATTAATACTAGATAATAAATCATATATTGCTGAAATTAAATTTGGAACATCAACAAATACTTATGATGCAGCAGGTGAAATTGTTTCATCTACAAACCGAATGGTTACATTAACTGAGGTTACTAAAATAGTTAAAGATTTTTATAAAAATGATTATTGACAAAAACCCCCACAATTTAGCGCTTTAAAAATCAACGGGCAAAAAGCTTATGTTTTAGCACGTCAAAAAGTTGATTTTGAAATAGCACCACGATTAGTTAAAATATTTAAATATCAAATTATGGATTTCAATTATGAAAAACAAATTTTAAAAATTTCTTTACATGTTTCTAAAGGTTTTTATGTTCGTTCTTTTGCTGTTGATTTAGCTACTAAAATTAATAATCTAGCTCATTTATTGACATTAATAAGGACGCAAAGTGGACCTTTTGAGATTAAAGATGCAATTGAAATTGAACAAGTCTATGATTATTGAAATAACATCAACAAATATTTATAAAATTAAGCAACAATTCGTCATTAATGAATTAATTATTGGTTTTTTTGATGGAATTCATTTAGGTCATATGAATTTATTATCAGATCCTAATAACCAAACTATTTTAACTTTTAAAAATATCCCACGTAAAATAAAAAAATTATATGATTTTAATGAGCGAATTCAACAATTAGAAGATTTAGGATTTAAAAGGATTTTTATTTATGATATTGATCAAAACAATTTAAGTGGGGAAGAATTTATTGATCAAATTTTAAAGCCACTAACTCCAAAAAAAATTATTGTTGGTGCTAATTTTACTTATGGCAATAATTTTTGTAATGCATCTAGTCTAAAACAATATTTTAATGTTGAAATTAAAATTATTACTAATGATGTTAGTACAACTAAAATTAAAGAATTAATTATAAATAAACAGGTTGAAATAGCTAATAAATTATTAATTAAACCATATTATCGTGTGGGAAATGTTGTACGTGGCGATCAAATAGCTCGTAATATTGGTTTTAATACTGCTAATATTTTATGTGATAATAATTTAATTGATATTGCTGAAGGTGTTTACAAAGCACAAGTAATTTTTAATAATAAAAAGTATGATAGTGTAGTTTATTTAGGAATCCCTAAAACAATTAATACTCGCTCTTTTTCAATGATTGAAGCACATATTTTAGACTTTAATCAAAATATTTATGACGAAAGAATTAAGATTGTTTTTTTGAAATATTTAGCTCCTAATTTAAAATTTAATAATATAGATGAATTAATCACTGCAATTAAAAATTATATTAAATTGGTTCTTGATAAAACAAATTAGGTATATAAACCAATAAATAATTTTTTATAGTAGTAAAATCTTAATGGCTATTATAAAAAAAACATAAACTTTCATGGTTTATTTTTTGTTTAAAGTAGTTGTATATTTAATTATTATAAATAGAGTAAGGACTAATCATTTTATGAAGATGAAAGCTAAGAAATTTTTAATTACAGGATTATTAGGTGTAGTTAGTTTAGCAACTATTACCACAGTAGCTACAGCTTGTACAAATGGCAATAATCAAAAGGCTAATACAGTAAATAATAAGTCTGATTTAAAAACAAATCCAAATATTAATTTAACTCAACAAGATGTAGAAAATTTGGAAAAACAAAGACAACAATGAAGTGAAGATTTAGATGTTTATTTTAATGGTGGGTTAAGTAAAAGTGGCAAAAATTTTGACCGTATAAATACATTATCATCAAATGCAACGGAATGATTTAAGAATCATCAAAAATTAGCGGCAACACGTGATTCTGGATCATTGGTACAAGATGATGCAGCTTATTCATTTGGAATTGCACCCGATTATGTTCATTATAAATGAGTTTCAAAAGATGGTGGCGACGTTTCTCCATCATATTTAAAACCATATTGAAATAAAAATTTAACTGAAGGTAGAAACTTTTCAAAATTAGACCCAAAGACTTTGGATGATAATGGAATCGGTGTTTTATTAATATCTGATCACCATTATGGTAAATGAAATGATAATTTTTTAAGCAAAAAAACTGTTGGTGGATTAATTATGCAATCACGAGTTTCTGGTTTGCCATCACACTATCCTGCTGTTCTAACACCAAAATTTTTATTTGATCCTAATTTAAATCCAAATTTAAAAAAATATGCTTCTGCTAATTATGCGTTACCTAATTATTACTCTGACCCACTAGATGGGATTATTTTAACAGGAAAAACTTTAGATCGTATTTATGATGCTAAAAAGTTTTCTAATGTTTATAATGCACATGTAGCTGGCAAAACACATTTTGAGACATTCACAGATTTTGCAAAGGCAATGGTTGATGAAACGCGTCGTGGAATTTCACAATGATCTTTAAAAAATGATAAATGAAAAGATAAAACAGTTTTAATGGTTATGCCTAACTTTACTCCAACAGCTAAAATGGTAGATGTTAATTTTAAACCAGAAGACTTAGAATTCTTGAGTAATGTTTGCATTAATGAACCTATGTATTGTCCAACAATATATAGTGATCCAAATGATAAATATTTACCTGGATTAGGTGCAAAATTCCCTATTCCAGTAAATCATATTTCACAAGTGCAAAATTATATTGATGATTATGGACAAATTGGTGGAAAAGTATTAAATGGTACTGGTAATCCAGAAGCATCAAAACAAGTTGGCCCAACTTTAGGTGATGCTTTTAAAGATACAAGTGACGTTGTAGTTTTCTGTTATAACGAATACGGTTTATTAAATTACAAACCCGGAACTGAAGAAGGCGAAGCTTTAGTACAACAATTTGAAAAGAATTTAACAAATTATGTTAATGGTTTAACAAAAGAAACAAAAAATCAATTTGTGCCAACAAAAGTTTTAAAAGAAAAACCAGTCGTGGGTAAGAATTTCTTTATTGAACGTAAAGGTAAGTTCTATGATGCATGTTATGGTTTTGCTGGTCAAGATTTAGTAGTTAATACATTAAATAAATGAACAAGGGGAGCTAACGCTCAAAATATAGATTTAGGGTATCCTATTTTTAATAAAAATACAGTTAAACACATTAGAGCATTTAAGGATTCTTTAGGTGTTAAAGTTTTAGACAAACAAAAATAGAGAAATAAATAGTTATGCAAATAAAACAAGTAAATATTAATAAAAAAGATCTTTCTATTTATAAAAAACTCATTGAAGCAAAAAAATTTGTTAGTACACAAATTCGTCGTCCTTATATGATAACGATTATTGTATTAACATTTATTATCTTAGCTGTTGGTATGTTTTTTATGAATGTTTTTTATGAAAAATCTACATATTTTGATGCGAATTATAATACTCATGTAACCTATTTTCGAACTTGAGCTAAAGTAGTAGCTGATGATAAAAGTATTTTATTGTATTGGTATCCACCTATCGTTAAATTATTTGTAGCAATTTCTATGCCGGTTGCTGGATATGCCATTCAGATTACAACTCAGAATCGTTTATCATCACCTTCAACATTAGGATACATTCCAGTTTCAATTTTGGCTTATGTAGCAATGTTGATGATTGATCAAGGTAAAAGCTGATTAGTATATGTTTTTGGGTTTATATTTTCATCATTTATCATTTTAGTTAATTATATTTTACAACGACAAAAATCTAGTAATCGTTCATTTAAACCAGTTTTAATTGGATTTGCTATATCTGCAACAATTACAGCGGTAGGTTTAGTAATTGCTGTTTCACGTCCAAATATTTTAAATCGAGTGACAATTTGAACTGGTGAATTACCAAATGTTTATGAGTGATTAAAATTATATATTTCAATGCCTTTAATTTTGATTTGTTTATTTGCTTTTTTAGTATTATCACCAAAATTAAAAATTATGCAACGCGATTTTGCTTTGGCAAAATCTTTAGGAATCAAAGTTAATTTAATTTTTTGAGTCGTGACAGTTTTAACAGCAATTGTAACAATTGCAACTGTTAATATTACAAGTCCCATGATTCTATTAGGTTTAATTATTCCAAATATAGTTAGGGCAACATTTAATAAACATGAACCATTATTTGTATTTTTTGTATCTATTGTATTTTCATTAGCATTGTTAGAAGTTTCATTATTTTTATCACTAAATTATCGTTTTGGTCCTAACTTTTTAATGGCTATTGTTAGTGCATTTGTGTTAATTTTTATTATGCGAAAACACGGATAAATAATATGAAAAATCCATCACGATTTATATTTATAATAAAAAATAATATTTTAAATACCATTAACAATGCATCATTTATTGAAAATGGAAATTTCAAGAAAAAATTTCGGGTTTATGCACGCAAGCAACATTTAGCAGCTTGAGTTATTAGTATTGTTACAATTCTTTTTTTATCGTTATTAGTTATGTTTATTAAAATTAATAACCAAGGTCAATTATTAAATAATCGGACTCCTTTAGATTTGTTTAAAGTAAATTATGAAACAATCTTACAAATTTGAATTACAGGAGCATCATTAGGAGCATCAGCTTATATGTTCCAACGGATTACTAAAAATCGATTAGTTGATACATCAACTCTTGGGATAGGTAATGTTTGTTTGATTGGGTTATTTTTTTTAGCAATATCAATTAATTTTGAAAATTATGGTTCTCAAAAACTTTTTGAAAATTTATTACCTTTTATTTTTATAATATCAGGAGTATGTGCCGGATTAATTTTGTATTGATTTTCAAAAAAACGAACCGGTTTTAGCCATACAAAGTTAATTGTCTCTGGTGTATTCATTAATTTTATTGCTATTGCTTTATCAATTAGCTTAAAAGACAATTTAACTCTAGTTGCATCAAATTATATTGACGATCGTTTGTTAGGTAATTTTTCAAACCGTAATAGTACTGAAAAATATTTAAGTTATGCTGTTTTTTTATTTTTAATGATATGATGCTTTTTGCGTTCTGCTAAATTTAAAGTTGTTATTACAGATACAATGATTGCTGAACAATTAGGATTAAAAGTAAAATTATTGAATTTAGAAATGATTTTTATCTCATCTTGTTTAACAGCTGTTTCATATATTATGGGAGGAAATGTATTATTTTTAGGAATTGCTGCAGCAAATTTAGGGTATGCGGTTTTTGGTAATCGCTTTAGTTCATCAATAATTTTTTCAAGCTTCATCACCATTATCTTTTTAATTTTTGGTCAATTTATTTTAGATAATATCGTTCAAAATTTAACTAGTTTGGTATTTGACGCCCCTATTGTAACTCCTTTATTAGTAGCTCCAATATTTACATTTATTATCTTATTTAAAAATAATATTTAAGCACAATCTTAGTAGTGAACAAGCCATATTGTTCACTATTTTTTTTTTTTTTTGGGTGGTAAATGGAAAAAAATATATTTATTTTTGTGTATTAAAATTAAAGGTATTAATAATTTTTTTAAATAAAAAATAAAGAAAGAATATTGTTATGAAATATAAAAAAATTTGAATTACTTCAATAGCTTCTATTGCTTTTATAACAACTATTGTAAGTACAACTACAGCTTGTAGTAAAGTTAATAATGAAGTTAAGTCTTCAAATGACATTTTTAAACAAATATTACCAACTAAAGAAGAACTTACAAATTTAATTAAAAGACGTAAGGATTGAGCTAATGATTTACAAGTTTATTTTGAGGGTGGAAAGTCTGCTTCAGGAAAAGAATTTGAGCGAATTCAAACTTTAGCAACAAATGCAAGTATATATTATCAAAAGAACCAAAAAATTGCTATGACGCGTGATTCTGGCTCATTATGTCAAGATGATGCCGCATATTCATTTGGAATTGCACCAGATATTTCTAATTATCGTTGAAAAGCAGGTGATGTAGAAGAAGTTGTACCACATTATTTATCTTACCTTTATAATCCTAAATTAACTAAAGGTATGGAACTTAAAGGTGTTAAAGCAGAAACATTAGCTGCTGAGAATGTAGGTACTTTATTTATTGCAGATTTTAAATTTGGAAATTATAAAACTAATTTTTTAGATAAAAATAAAATAGGTGGAATTGTTATGCAGTCACGATCATCAGGTTTGCCTTCAAAAACACCAGCTTTATTCATGCCTAATTGGTTATTTGGTAAAAATAACCCTTATTTAGAGACTGACGTTAACGCTTCAGCTGGAATTACAGATTTTTTTGTTGATCCATATGATGGCTTAATCTTTACAGGTAAAACCTTAGATCGCATTTATGATGCAAAAAAATTTGATAATGTTAATAAAGCGGTTATTGATGGTAAAAGCAATTTTCAAACATTTACAGATTATGCAAAAGCAATTGTTAGTGTTGTACGAAAGAATGTTTCGAATTGAGCTAAACAACATAGTTATTGAAAAAATAAAACTGTTGTTATGACAATGCCAAACGTAATGAAAGGAGCACGAATTATTGATCCTAATTTTAGTAAAGATGATTTACTATTCTTAGGTAATATTTTTATTCCACAACCTGTTTATTTTCCAATTTTATATGCAGACCAAAATGATGAATATACACCTGGATTAGGAGCTAAATTTCCTATTCCTAAAAAAGATATAAATGAAGTTCAACGCTATGTAGATAATTGAGGTTGAATAGGTGGAGCAATTTTAAGTGATGCTACTAATCCTGATGCTGCAAAACGTGTTGGTCCAACATTGGGTGAAGCTTTTGAAGGAACGGTTGATAAAGTAATTTATTCATATAATGAATATTTAATTAAAGGATATAAACCAGGAACAGCAGAAGGTGAAGCTATAATTAAAAAATTCGAAGCTAATTTAGCAAAATATGTAAATAGTTTAGATATAAATACAAAAAATAGTTTTAATCCTACAAGGATTTTAAAAGAAAAGCCAGTGGTAGGTAAAAACTTTTTTATCGAAAGAAAAGGTAATATTTACGATGCTAATTATGGATTTATAGGTCAAAAAACTATGGTTAATATTTTAAATCGTTGAATGAATGGTGAAAATAGTCCAGAAGTTGATTTGGGTATTCCTAATTTTACTAAGGATAATGTTAAACATTTACGTGCTTTTAAAGATGAATTAAATGTTAAAAATACTGATGAATAATAAAAATTTTACGCATTGGAAGTTTTTATGCATAAATTTTTTAAATTTATTAATTAATCATTTAAACTTAAAAAAGGAATTACTAAAAATATATGAAGTTGAAGTTTAAAAAAATCTTATTATCTATATCAACTAGTTTAATAATTTTATCATCAATTATTACAATTGCTGCATGTAGCAATCAAGTAAAATCTAAAACAAATTTTTCAAAAACAACACCAACTTTAGAAGAATTGACTGAGCTTAAAAAAAGAGAAGAAGCATGAAAAGCTGATTTAGAAGTTTATTTTGAAGGTGGAGTAAGTAAGTCAGGAAAAAAATTTAATCGAATTGAAACTTTATCATCAAAAGCTAGTTCTTATTATCAAAAAAACCAAAAAATTGCGGTTACTAATGATTCTAGTTCGTTATGTCAAGATGATGCACTATATGCATTTGGAATCGCACCTGATTATTCTGGCTTTCGTTGAAAAACAAGCGAACTTGAAGAAGTACATCCTCATTATTTAAATTATTTATACAACTCTAAACTAACAAAGGGAATGAATTTCACAGGTAAAGGAGCATTACCAGAGAATTTTGCTAAATTAAATGTGGGTACATTATTTATTGCTGATTTTAAATTCAATAAGTATAAAGAAAATTTTTTAGATAAAAATGAAATTGGTGGTGTAGCAATGCAATCACGATCATCAGGTTTGCCTTCAAAAACACCAGCCTTATTCATGCCTAGCTGGTTGTTTGGTAAAAATAATCCTTATTTAGAAACTGATGTTAATGCTTCAGCTGGAATTACAGATTTTTTTGTTGATCCATATGATGGCTTAATCTTTACAGGTAAAACCTTAGATCGCATTTATGATGCAAAAAAATTTGATAATGTTAATAAAGCGGTTATTGATGGTAAAAGCAATTTTCAAACATTTACAGATTATGCTAATGTTGCTGTAAAAGCTGTTCGAAAAAGAGTTTCACAATGAGCAAAACAAAATAATAAATGAAAAGATAAAACAACGGTTATGATTAGTCCTGATGTAATGCCAAATGGTCGAATTGTAGATCCAAACATTACTAAAGAAGATTTATTACATTTAAGTAATTTTTTTATTACCCAACCCGTTTATTTACCAATTTTATATGCTGATCAAAATCATCCTTATACACCTGGAATGGGTTGTAAATTCCCTATTCCTAAAAAACACTTAGAAGATGTACAAAAATATATTGATAATTGAGGTTGAATAGGCGGTGCAATATTATCATCTAGTGCAGAAAATCCTGATGCTGCAAAACGTGTTGGTCCAACATTGGGTGAAGCTTTTGAAGGAACGGTTGATAAAGTAATTTATTCATATAATGAATATTTAATTAAAGGATATAAACCAGGAACAGCAGAAGGAGAAGCTGTAATTAAAAAATTCGAAGCTAATTTAGCAAAATATGTAAATAGTTTAGATATAAATACAAAAAATAGTTTTAATCCTACAAGGATTTTAAAAGAAAAGCCAGTTGTTGGTAAAAATTTCTTTATTGAACGTGAAGGAACATTCTGCGATGGTTGTTATGGTTTTATGGGTCAAAAAACAATTGTTAATATTTTAAATCGTTGAATGAATGGTGAAAATAGTCCAGAAGTTGATTTGGGTACTCCTAATTTTACTAAGGATAATGTTAAACATTTACGTGCTTTTAGAGATGAATTAAATGTTAAAAATACTGATGAATAATCCTTCGTAATTATAAAAAATGTGAATTATTTCTTTATATTTATAAGAAAGCGTTCACATTTTATTTATTATTTTTAGAGTTTATCTAAAAATTTAATTCTTACAAAAAGAATTAAAGTAGAATATATATTAGTTTTATAAAAATAAGGATTAATTAATGAAAAATAAGTATTTTAGAAAAAATAAATTTTGGTTATTAATACCAATAAGTTTTGTAAGTCTTGGAAGTATTACATTAGTTGCTACTGCTTGTGTAAAGAAAAATTTTTATCCTAATACATTTGCAGATGATCAGATTTATATCACTAAGAATAATGAAAAAACTGTTAAGTTTCGTATTAGTCGTAATGATGAAGAAAATAAAAAGTATTTTAAAGATGATCTTAAATGAAATACTTTCATTAATAAACTTAAAAAAACTTATATTAATGTTGAAACTGTTATTCCAAAAGAAAACGCAAAACAAAATATTAAGCAACAAGATTTATGCATGCGCGCATTACCTGTACAAGTTTTAAGTAATAATAAATATGTTGATATTTACGTAAAGATTCCTGTTTTATTAGCTAATACTAAAATGTTAGTTGTTGGTGATGATTTACTAAAATCATTTTATTTTGATACAGATAACTTAATAATGAATGCTAAACATTTTCAACCAATTCCTATCAAAATGCTTTAGTTTGTTAATAAATAAAAACCTCTTTTTAAAGAAGGAGGTTTTTATTTATTAATGATTATTCCTTTGATTGTTGTCATTGATAAAATTGCATCAACAATACCTTGAACACCAAATCCAGAATCTTTAACACCAAAAAAAGGTAAAATATCTGGACCACGTGAACTTGATTTATTAATGTTAATTGTTCCTGATTCAATTTGTAAAGCTAATTGTTCAATACTTGCATAATTTGTTGTAAAAATACAAGCTTGTAAACCATATTGTGATTGATTAATTAAATCAATAGCTTCTTGTATACTATTATAAGTTATAATTGGTAAAATAGGACCGAATGGTTCTTCTCAAGCTACGCGCATTTCTTTTGTAACATTATCAATTACTAAAGGATGCAAAAGGTTATTTTTTTCATTATAAACGATTTTTTGGTGTACTATTGCTCCTTTTTTGATTGCATCCTCAACTAATGATAAGTTATATTTTAATGAATTAGAATTAATTAATGGAGTAATTATGGGATTTTGTTGTGGTAAACCGACTGTTAAAGCGTCAACTTTTTTGTTAATTAAATTAACTAATAAATCATGATTTTTATGCGAAACAAATACTCGTTTGATTGCAGTACATCTTTGCCCGCTAAAGCCATAAGCACCTTTAACTATTTCATTAGCAGCTAGTTCAAAATCCGCATCATCTAAAACAAGGGCTGGATCTTTTCCTCCTAATTCTAAAACTAAAGAAATCTTTGATGTTATTTCAGCAATATGATTGCCTACTTTAGGTCCGCCCGTAAAGCTAATCGCTTTAACATATTTATTAGTAACTAGTAGATCACCTGTTATACGTGCTTCAGTTGTTAAGCAGTTAACAACTCCTTTTGGAAAACCTACTTCATGAACTAATTCACTAATTCGAATACCAATTAATGAACCTTGTGTTGATGGTTTATAAACTAAACTATTACCAGAAACTAATGCTGGTGCTAATTTTGAAATCAATAAGTTAATAGGATAATTAAAAGGACAAATTGCTAAAACAACACCAACTGGAATACGATAATATGTTGCCACTTTGTTTTTAACATGGTGAACTGTTTCATCAAAAATAATTGGTTTTTGCATTAATTTTTGATACTCAAAAACTGTTTCGCGTAAATAATCAGCACTTCGTTCAACTTCAATTAGGCTATCTTTTAATCCTTTTGCAATTTCAGTACTAATAATTTGTGCTAATTCTTGTTTATGTTCATCTAGTTTATCGGCTAATTTTAAAAGTAGATCACAACGATATTTAATTGGTGTGTCCTTAAATTTTAGATATGCTATATGAGCATTTTCAAAAATAGTATTAATTTCATTTTCATTATGAATGTTAGGAACATAAGCAATAATTTGATTATTAGATGGATTATAAACTGGTAGTTTTTCTTTAGCATCAACAAATGCTCCATTAATTAATGTTTTATAGTTCAAATTAAAAACCTCCTTTATTATTTAAATTAAATTATAGTAATTTAATCCCTTTTTTTAACAATTCATCTTTATATTCATTTGGTCAAACTGAAACTTGTACCTCACCAATGTGTTTTTTGTTTAATAAAAACATACATAAACGACTTTGACCGATTCCCCCACCAATTGTTTGGGGTAGTTGATTATTTAAAATCATTTGATGATAAAAATTATTTTCTTGATCATTTTTTAAATTAGCAAAATTTGTTTGCTTAATAAAATCTTGTTTGCTAACACGAATTCCCATTGATGATAATTCAATGGCTTGATCATTTAATTTATGATAAACAATTAAATCTCCATTTAAATTTCAATTATCATATTCTGGAGAACGTATTGATTGAATATAACCTGATTTTAGTGGATATCCAATTTGGTATATAAAAATTGCTTTATGAATTTTTGCAAACTCATATTCACGTCGAGATGGTGATAAATGTGGATATAAATCTTCTAATTCTTGTGAACTAATAAAAGTAATACTATCTGGTAAAATAATTTGTTTTGGATTATATAATTGATCAATTTCTAATTGAACAATTCTAATTGTTGAATAAATTTTATTAACGATTGATTTTAAAAAATCTAAGTTACAATCTTGGTTATTAATTAAAATTTCTCAATCTCACTGATCAACATAAATGCTATGAATTTCATCGATGTCATCATCTGCACGAATTGCATTCATATCAGTATACAAACCTTCATATAAACTAAAACCATAATCTAATAAAGCTAAACGTTTTCATTTTGCTAGTGATTGAACAATTTCAAGTGTTTTATCTAATTTTTTAGCATAAAAACTTACTGCTTTTTGTTTATGGTCTAAATGATCATTTAGACCTGATTGTCCTTCAACAAATAATGGTGCTGTTACACGTGAAAGATTTAAATTTTTTGCAAAATGTTTTTGAAAAGAATTTTTAATCTCTACAATTGCTTTTTGTGTTTGATTAATTTTAGCTTTTTGTAATTGATCCACGTTTTTTAACTCCTTTGTAAAAATTAATGTAGTAAATAATACCACTGATTACAGATAATGATGTTGCAAAAAATAAAGGAATATTAAATAAATAAACGTTTTTTCATGATGAGGTTAAGCTTAGTAAAAATAAAATAAAGCAAATACTAATAAATAGTAGAATGGATTTAATTTTGCCAAAAATATTTGCTGGAATAATAATTTTCTTACTTGAAGCAAACATTCGTAAACCATCAATAAAAATATCTCGTAAGATATTAATAATTGTTAAATAAGCTAGTGCTGTATGATCTAGTGTTAAACAAATTAAAACACCATTAACAAGGATTTTATCAGAAATAGGATCTAAGATTTTCCCTAAATTTGAAACTAAATTATATTTACGAGCTATATATCCATCTAAGAAATCAGAAAAAGCAGCAATAATAAAAATAATTGTTGCACTTAAACGCAATGCACTAATATTGGCATCTAAAACTTCATAGACAATATTTAAATTTTTTGTGTAAAAATCAACTAATAATAAGATGATGCAAATAAGTGCTAAAAAAATTCTAAAGATAGTGATAATATTAGGAATGTTTCTAAAAAAAGGTGATGATGAGATGTTTTTTTTAAAATTCAATTTGGCCATGACTTCGTGGGAATGGGATTGTATCTTTAATATTATTAACTCCTGTTACATACATCACTAAACGCTCAAAACCAATTCCAAAACCTGATGACATATAATATCCGAAACGACGTAAATCTAAATATCATTGTAAACTTTCAATTGGCATTTTTAATTCTTGACATCGTTTTAATAGTTTCTCATAACTATCTTCACGTTGTGATCCACCCACTAATTCGCCAACGCCTGGAATTAATAAATCTGTTGAAGCTACAGTTTGTTGATCATCGTTTAATTTCATATAAAATGCTTTAATATCCTTTGGATAATTTTGTAAGAACACAGGACCTTGATGATATGTTTCACACATGTATCTTTCGTGCTCTGATCCTAAATCCATTCCAAAAAAGATTTCATTATTTTCGAATTGATGACCATTTTTAACAGCATCAGCTAGGATTTTAACTGCATCTTTATATTCAATACGTTCAAATTTAGACTCAACAACTTTTTGTAGTTTTTTAATTAAATTTTCATCATTTCTTTGAGCTAAAAATTCTAATTCTTGTTGATTTTTTTCTAAAACAGCCTTAATAACATGTTTAACTAAATCTTCAACAATATCTTGCATACCTTTTAAATCAACAAAAGCCATTTCAGGTTCAATCATTCAAAATTCTGCTAAATGACGATTTGTGTGAGATTTTTCAGCTCTAAAAGTAGGACCAAATGTATAAACATTACCAAAAGCTTGTGCGTATGCTTCAGCTTGTAATTGACCTGTTACAGATAAATGAGTTTGTTTACCAAAAAATTCTTCATTATCAAATTTTTGAATACTAAAAGTTTCGCCAGCTCCTTCAGCATCATTTCCTGTAAACAATGGTGCAGCTAATCAACTATATTCGTTTTGATTCATAAATTCATGAACTGCAAAAGCAAGTGTTGCCCTCACTTTCATAATGGCATGAAATAAATTTGTTCTTGGTCTTAAATGAGCATTTAAACGCAATGTTTCTAAATTTAAATCATTGTTTGATAATAAAAAATCTTCATCACTTTGTTTTAAAATTTGAGCATTTAATACTTCTAGTTCTAATGGTTGTTTAGCTGTTGGAGTTAATTTAATTACACCTTCAATAACAACAGCGGCTCACATTCGTGCTGCAGCTATTTCTTCATAATTGTCAATTTTATCAACACGATATACAGCTTGTAAATTTGTTAAAGAACTACCATCATTTAGAACTAAAAAACCAATTTTTTTTGAATCACGGTTTGAACGAACTCAAGCTTTAATTTGTATCTTTTGGCCTTCAAGTTTTACATAGTCTTGATTAAAAATTTCTTTAATTTTTAATTGCATAATAAAATCATTACCTTTTTTTTCTTTTTTAAATAATATATAAATAAAAAAGCAAAACATCATTGATTAATAAGTTTCGCTTTAAATTAGTTTTTATTGCTCGTTTTTTGCTTCTAGTTTTTGTTGCTTTTCAAGACGCTTTTGTTCACGTTGACGTTTCTTTTCTTCTTGTTGTTCATCAAAACGCTTTTCAGCAGCAACCTTATCTTGTTGAGTTTGTTTTACTAATTCAAAAATCTTTCAACCAGCATCTGACATACCATTATTTTTTAAACGACGATATTTAACGTATTTGCCAGATTTAACAGGTGTTTTTGGTTCTTTTTCTTCTTTAATACTTTCTTGTTTATTAACTTGATTATCTTGGTTAACATAAACAATTTTCTCAACAATTTTTTCGATTGGCTTTTCAACTTCAATAACTTTTTCAATATAAACAGGTTTTTCAACTTCAACAATTTTTGTATTGCTTAGGTTTGTTAAATCATTTAAATCAACACGTAATTTAACAATTTGTGTTTCATGATTTAATGACACTTCTTGTTCATAAACATTATTAGTTTCACCATCATCACTATCATAATTTTCGTGTTCACAATAATGGTTATATTCAATTGTTTTTAATTTTCTTTTTAAATTATCAACTTTATTAGCTAAATCACGAAGCTCTAATAATTCGCTAATTTGTGGATCATATTTTTTATTAGTTGCAGACATTTGTTCCTCCACTAAACGACTTGGACATTTATATTTAAATATAACATTAAAATATTATTAAATATTAATAATTTTAACATAATATAAGGAATTGAAATGTGGTTATGATCATCAAGAATTAATAATTTATGAAATTTATTTATTCTTCATCATTTTCATCATCACTAACTTCTAACAAAAGTTTATTTGGATCAATTTTTCTAATTGTTAATTCATTGATTTTCTTTTTTAAAGTATTTAGTTTTTTGTTAATAATATCTTCAGCACTTTCTTCAATACTTTCTGCTTTTTTAATAATAGCTTTTGAATCATCGATTATTTTTTTAGTTTTATCATTGATGTATTTTAGAGTGTTATCAACAATATCTTTTTTTAATTCATCAAGATTTTCTTCAATTTTTGCACGATCTTCATATCTTATGATTTGTGAATTAATTTTTGCTTTAAGGGTTGCAAAATTTCGAATCATATCAACTAAAGGAATGAAGTATTGTGGCCGTACAGCAAACATATTTTTGTATTCATTAATTTTTTTAATTACAAAATGATCTTCTGGTTCTAATTCAGTTACTAAAATTGCATATTCAGATTTTTGATTAATACGATCTTGATCTAATTTTTTATAGTGAGCACTATTTTTTGTACCTTTTTGATTGTCAAAAAATTCTGTTTTCGCTTCAATTGTCGCACTAAAAATTAATTTTTTATCATCATTTGACATTTCTTTACCAAAATCAAAAAATTCTAATAAAAAGTCAGCTTTTTTACCATCAATTGCTTCATTAATTTTAGTGAATGTCATATCAGGACATGAAAATGAGTAAGTTTCATCAAATTTATTTTTTAATCATTGTTCAAGTTCTTCACCAACAAGTTTAACATTTGATGATTTTTCACGTTTTAATTGTTCAATACTTTCTGTTAATTTATGGATCTCTTTATCTTTTTTACTTTCAAGTTCCTTAATTTCAACAATTTTTTTAGACTCTCAATTTTCTTTAGCAGCCTTCATATTATCTTCATGAGCTTGAATAGCAAATTTTAATTTTTCTATTTCAATATCTAAATCTATTTTATCTTTAATAATTTTTTGATATTCATTACTTTCTTTAAAATTAATAATTGCCATTTTTTCAGCATTATCTAATTTAATTTCTTGTTTAGCAATAGTATTTTTTAACGATTTTGTTTCTTCATCAAATTGGCTTTTTAACTTTAATTCTATATTTTCAATTTCTTTTTTGTAGCTTTGTTCATTACTTTCAATTGTCTTATTTAAAGAATTAATTTTTTCTTGTGCTTTAATTAAATTTTTGTATTCATCACTATCTTTAAAATGAGCAATTGCTTTATTTCCCTCATTTTTTAATTTTTCTTTTAATTCATCACGTTGTTTTTCTAAATCATATCTTTGTTTAACTAACTCATTGTAATCACGATCATTAGCTAAAAAATTATTTCTTCAATTTTGTTCTTCTTCTTCACGAATTTTTTTGATAAATTCATCACGTTGTTTTTCTAAATTTTGATTAAAAGATTCATAAATAGTTGAATTTTCATCTTTGAAAACCAAAATCTCATTGGTATTAATCGGCTCAAGAATTTCAAATTCTAATTTTTCAATATTAATTAATTTAATTTTTTTATTAGCTGTAGTTTTCATAAACGACTCCTTTGTGTTATTTTAGGATCATACTTTTATTATAATGTGTTCTAAAAAATATCATCTTTAAATAAATATTTATTTTTGGTACATTAGTTAATAATTAATGTTTATTCATAATTAAAATCTTAATCCTTAATTTATTAATATAGATTAGTAAAACTCACTTATTTTAAAAACTTTCGTGTTTTTCTTTGCTAAAATTAAAGATAAGTTTTTAAAAATTAAATTGGTGAAATTTATGTTAAATGTTGTAGAAATTATTAGCAAGAAAAAAAATAATATTGAATTAAGTGAACAAGAAATTAAATTTGTTTATGATGGTTTTGTTAATAAGACAATTCCAGATTATCAAATGGCAGCTTTTTTAATGGCTGTTAATTTTTGTGGATATAGTGAAAATGAACAATACTATGCAACTAAAGCAATGGTTGAATCTGGTAAATTATTAGATTTGCGTGTTGAAAATAAAATTGTTGTTGACAAACATTCATCTGGTGGTGTGGGAGATAAAGTTTCAATTATTTTAACTCCTTTACTATCAGCTTTAGGATTATACGTTGGAAAAATGTCTGGTCGAGGATTAGGGCATACTGGTGGTACAGTTGATAAATTAGAATCATTGAATTTAAATTTAGATTTTGATTTAAAAACATACATGAATCAGCTAAAAGATAATGGTTTATTATTAACTGGTCAATCAGATGATATGGTTGTGGCTGATAAATATATTTATGCATTACGAGATGTAACAGCAACAAGTGATGTGTTTGATCTTATGATAGGATCAATTATGGCTAAAAAATTAGCTTTAATTACTGATTATATTTTCTTAGATGTTAAAGTTGGTGAAGGAGCATTTTGTAAAAATGTTGCACAAGCAGAATCTTTAGCAACAAAGATGTTAAAATTATCAAAGAAATTTAATCGTAATACAATTATCCATTTAACAAATATGGACCAACCTTTAGGAAAAGCAATTGGTAATGCTATTGAAATTAAAGAAAGTATGGATTATTTATTAGGTAAACCAGTGCCCCAAGATTTATATGATTTAATTAATCAGTTTGCTTTAGACATCTTAATTGATACAAAATTCGCTAAAAACAAGGAAGATGCTCAATCTAAGATTGATAACGTCATTAAAAATGGTTTAGCTTATCAAAAATTTGTGAATTGAGTAAAATGATACAAAGGTGATTATATTAGTCTTGAAAATGATACATATTTTAACCCTAAATACAAATTAGAGATTTTAGCAAAACAAGATGGATATTTAGACTTTAAATCAACAAAAGAATTGGGTATGATTGCTGTTGATTTAAAAGCAGGACGTAAAGTTAAAACTGATCAATTAGATTTTCAAGCTGGAATTTATTTAAATAAGAAAAATAATGATTTCGTTAAAACAAATGAAGTTATTGCTACACTTTATGCTAGCGAGCCAATTAATGATGAAGTTGTTAATAAATACCATAACAATGTAGTTTATTTAAAAACACCAAAACAAATTGCACCATCAATTATTAAAGTAATGCGTTAAATAATAAAAAAGTAAGGAAGAATATTTATTATGCTGAAATTAAGTACGAATGAGATTAGAAAAAAGTGAATTGAATTTTTTGAGTCAAAGGATCATTTATTTATTGAACCAAAGTCATTAATTCCCAAAAACGACCCTACACTATTATGAATTAATTCTGGTGTTTCAACCTTAAAAGATTATTTTAGTGGTAAGGTTAAACCACCACATAAACGTTTAGTTAATTCTCAAAAAGCTATTCGTACAAATGATATTTTTAATGTTGGATTAACGAGCCGTCACCATACTTTTTTTGAAATGTTAGGGAATTTTTCAATTGGTGATTATTTTAAAAAAGAAGCTATTGATTGAGCTTATGAATTTTTAATTAACGTTTTAAAAATTGATGTAAAAAAACTATGAGTAACTGTTTTTGAAGATGATCAATTTACGAATGATGAATGAATTAAATTAGGAATTATTAAAGAGCAAATTATTAAATGCAATCGTGATCGTAATTTTTGAGATGTAGGTAATGGCCCTTGCGGCCCATGTACTGAAATTCATTATGATCGTGGTGAACGTTTTGACCCAAATAAAATAGGATCAAAATTAATTTTAGAAGATATTGAAAATGATCGTTATGTTGAAATTTGAAATATTGTTTTCTCACAATTTAATAATGATGGCCATAACAATTACACTGAATTATTACAAAAAAATATCGATACAGGAGCTGGCTTGGAAAGAATTGCTTGTATTTCACAAGATGTACCAACTAACTTTGATAGCGATGTTTTTATGCGAATTACAAAAAGTGTTGAACAGTTTTCAGAATATAAATATGACATGAATGAATACTTTCATCCCAATGTTACTCAAAATAAAATTAATTTCGCTTATAAAGTAATTGCAGATCATATGCGTGCAACTGTTTTTGCGATTGCAGATGGTGCAATTCCTTCAAATAAAGAACGAGGTTACATTTTACGTCGTTTAATTCGTCGAACTATGGTTTTAGTGCGTCGTTTAAATATTAACAATCTTTTATGAGTTGATGCAGTTGTTGACGCTATCGCTTCAACAATGGGTGATTTTTATACTTATTTAAAAGATGAAAAAACACTTACTAAAATTAAAATGATTTTAAATAAAGAAGTGCAATTATTTGAAAAAACTTTACAATTAGGTTTAAATATTTTTGAAAATTCTATTCGTAATCAAGAATTAGATAAAGAAATTACTTTTAAACTAGTTGATACATATGGTTTTCCAATTGAATTAATTAAAGAAATTTGTGAACAACGTAATGTTAAAGTTGATTTAGAAGCTTTTGATGCTATGTTTAAACATCACCAATTAATTTCAAAAGCAAACAAAGCTAATTTAAAAGTAATGGAATCACAAAACGAATCACTAATGCAATTAGATGTTGATTCAACCTTTCACTATGAAATATTTAGATGAGAAAATGCTAAAATCATTACTTTATTTAATGAGGATTTTGAATTGGTTGATGGTTTAGATCATGAAGATGGTTATGTTGTTTTTGATAACACATGTTTTTATGCAACATCAGGTGGTCAACAACATGACACAGGTTATATTATTAAAAATGATCAACAATTTTTTGTTGATGATGTTTTTAAGGCACCTAATCGTCAACACGTTCATCATGTTAAAAATGCTAGTTTATCAATGAATGAGCATGTTATTTTACAAATCAACGAGCAAGATCGTAAATCAATTACAGCAAACCATACAGCCGAACACTTATTACACTATTGCTTAAAACATGTTTTAAGTCCTGATATTAAACAAGAAGGAGCTGCTAAATATCCACATAAAGTAACATTTGATTTTACTTATCATGCTCAACCAACAAAAGCACAATTAGATAAGTTAGAAAATGTATTAAATGAAATGGTACAAAGTAATTTTGATGTACAAGAATTACACATGGATTTAGATGAAGCTAAAGCTGTTGGTGCTGCTGCTTATTTTGAAGATGTTTATAAAAAATTAAAAGGCAAATTACGTGTAATTAAAATGGGACCTTCAATTGAGTTATGTGGGGGTACACACGCACATCATACATCAGAAATCGAGCGAATTAAAATTGTTGAATGTGCTTCTAAAGGGGCTGGCTCTTGAAGAATTACTATGGTAACAGGTCATGATAATTTAGCAAAATACATTCATGATTTATATGTAGACTACTTAAATGAAATCAATCACTTAAAAGTTAATTTAGATATTAATGATCATAAATTAAATGATTTATATAATGCTTTTGCAAATTGAAAAAATCTATCAATTGATGATTATGATTTATTAAATGAAAAATTTGCTGAACTAAAACAATCATTAATTAATTTTAAAATCGAATTTGATAAACAAAATGCCAAACAAGCTATTATTGATATTAAAAACACTTTTAATACACAACTAACTAATAAACGTGTACACGTTTTTAAAAACACTGATAATAAAAATATCTTCAATGCTTTAAATGAATTAATTAACGAAAATCAAGATACATTATTTATTAGTTTCAACTTAGATGATAATAAAATTCAATATTTATTAGCAATTAATGAAAAGTTTGCTACTACAAACCAAATTAATTTAAATAAATATATTAAAGAATTAAATACTATTTCTAATGGTAAAGGTGGGGGCAAGCCATATTTTGTTCAAGGTGGAACAAGTGAACAAGAAAAATTAGATGAGTTATTAACTGTTGTAGATAAATGAGTTATTAATGCGTAAATTAGCACTTGATTTAGGAACAAAATCATGTGGTTTTGCAATTAGTGATTTATTAGGAATAATTGCTAGCGGACTTGATAATTTTATTTATGAAGAAAATGATTTTACAGCTGTTTTAGCTAAAATTGATGAAATCATGATTAATTATCATCATGAAATTGACACAATTGTTTTAGGTTATCCAACTAATGTTTATGATGGATCAAAAAATGAACGAACATATTTAATCGAATCATTTTATGCATTATTAAAACAACACTTTTTAAACCATGAAAAAATTAAAATTGTTTATGAGGATGAACGTTTTAGCACTAAAATTGCAACTCAACGTTTAAAAAATAGTTGTGTTAAAGCAGCTAAAATCAAAAAAGTTAAAGATAAAATGAGTGCTGTTGTTATTTTAGAAAGTTATTTAAGTAAAAATCATTTTAATTAAAGAAAGGTAAGACTTATGTATAATCACAATAAAATTGAAAAAAAATGACAAAAGTATTGACTAGATAATAAGACTTTTAAATTTGTTGATAATCCTAACAATCCTAAGAAATTTTATGTATTAGACATGTTTCCATATCCTAGTGGAAAAGGATTGCATGTTGGTCATCCCAAAGGTTATACAGCAACTGATGTAATTAGTCGTTTTAAACGTTTGAATGGTTATGATGTTTTACATCCAATTGGTTGAGATGCTTTCGGTTTACCAGCAGAACAGTATGCATTAGAAACAAATAATCACCCTCATACTTTTACGCAACAAAATATTAAAATTTTTCGTAAACAATTACAAATGATTGGTTTTGATTTTGACTATGATAAAGAAGTTGATACAACCGATCCTCAATTTTATCAATGAACGCAATGGATTTTTGTGCAATTATATAAACATAATTTAGCTGAAATTCAAGATATTGATGTTAATTGATGCGAAAATTTAGGAACTGTTTTAAGTAATGAAGAAGTTGTTTTAAATGATAAAAACGAACGGGTAAGTGAGCGAGGTGGTCATCCTGTTGTTCGTAAACCAATGAAACAATGAGTTTTAAAAATTGTAGATTATGCTGATAAATTATTAGATGGTTTAAATGAAGTTGAATTTAGTGAATCATTAAAATCGTTGCAACGTAATTGAATTGGTAAATCAATCGGTACTAGTGTGCAATTTAAGATTAAAGATTCACTTTTAACACTTGATGTGTTCACTACACGAATTGATACTATTTATGGGGTACAATACTTAGTAGTTGCACCAGAACATCCAATTCTTAAATCTATTACTAGTGAGCAACAAATTAATGTTGTTCAATCATATATTGAACAAACAAAGAAAATAAGCGATTTAGATCGCATTGCTGATACTAATAAAACTGGTGTTTTTAGTGGTGCATATGCTATTAATCCAATCAATCAAGAAATCATTCCAATTTGAGTAAGTGATTATGTTTTAATGAATTTTGCAACTGGAGCTGTTATGGGTGTACCAGCACATGATGAACGTGATTATGCTTTTGCTAAAAAATATGCATTACCAATTAAAAGTGTAATTGATACAAAACAAAAATTACCATATGCAGGTGATGGTTTACATATTAACTCAGCAATGATTAATGGTTTAAATATTAAACAATCTCAAAATGTTTTAAATGATTATTTAATTAAAAATCATCTAGGAAAAAAAGTTGCAAACTATAAATTGCGTAATTGAATTTTTAGTCGTCAACGTTATTGAGGTGAACCATTCCCAGTTTTATTCGATGAAAATAATCAAATAAAGATTATCGAAGACTTACCTGTTTTATTACCAAATTTAAATGAATTTAAACCTTCTAAAACTGGTGAATCTCCCCTTGCAAATGCCCAAGAATGATTATATGTAGAAATTGATGGTAAAAAATATCGTCGCGAAACAAATACAATGCCACAATGAGCAGGCTCATCATGATATTTTTTAGCTTATATTTTAAAAAATGAAGATGGTTCATACACACCATTAAATAGTGAAGAAGCTAAAAAGCGTTTTGCTAAATGATTGCCAGTTGATGTTTATATTGGTGGACAAGAACATGCTGTTTTACATTTACTATATTCGCGTTTTTGACATCGTTTTTTGTATGATATTGGTGTAGTGCCAACAAAAGAACCATTTTATAAAGTAATTAATCAGGGAATGATTTTAGGCGAAAATAATGAAAAAATGTCTAAATCAAAAGGGAATGTTATTAATCCTGATGATATTATTGCTTCGCATGGTGCTGATACATTAAGAATTTATGAAATGTTTATGGGACCATTAACAGCTTCATTACCATGAAATCCGGATGGGTTAGATGCGATGCGAAAATGATTAGATCGCGTTTATCGTTTATATCATAATTTAAGTGAACTAGAAGTTGTCGAAGATCTTAATAAACTAAATGAAGAAATAATTATTGCCTATCATACTTTAATTAAAAATTACACAAAAGCAATTAATGAACAAGCATTTAATATTGCGATTAGTGAAATGATGGTTTTTGTTAATGTTTTATACAAAAATAAAGTTATTAATTATGAATTATTAGATAATTTTTTAATTTTACTTTCATGCTATGCACCACATTTAGCGGAGGAATTATATAGTTTAAACCATTCAGAATCTGTTTGTTTACAAAAAATGCCTATTTATGATGAACAAAAAATTATTGCTCAAAATATTACAATTCCAATTCAAATTAATGGTAAATTAAAGCACACAATCAATGTTTTAAGAGATACAAATGCTGAAGAGTTAGTTAATTTAGCATTGGCTTGTGAACAAGTTAAACAAGAAATTGGGGATCAACCAATTAAAAAACAAATCGTTGTTGTTAATAAAATTATTAATTTTGTTATTTAATGTTTTTTACTATGTAAAAAGACACTAATATTGATTTTTATTTACAATAAAAAAATAATTAACAAAACCCCAGTATTTTTTTCAATAGGCTCATTTTAATGATAGATTAAACCTATTGTTATAAATATTTATAGCTTGTTAAAGCAATTACTAACAAAATAAAAAACCACCATTGTAAACTAGGACAAAAAATTTAGACTCAAATAGATTTATTGTTCATATAAACATCCCACATTTGTTGGGGTGTTTTTAAATCAAATTTTTTGATAAATCTTTCATTATTATACAAATTAATAAATTTGTCTATTTCTATTTGCAATTCATTAAATGTTAAACTTTTTGCTTTTTGAAAAAAATTTGAAAATATTTCAGTTTTTAGAATAGAAAAGAAATATTCTACTTCTCTATTATCCAAAGAATTTCCAACTCTTGACATAGAAATAGTGCCATTAATTGAATCTATGTAATCAAGGAATTCCTTAGAGGAATATTGATATCCGTGATCTGAATGTACAATGAATTTTTTAGGAAATTTAGTTAGTTTAAAATGTTTTAAAACAAGATTAGTATCATTATATTTTGATAAATTCCAGCTCACAATTTTCTTTGTTTTATGATGCAATAAAACCGATAAATATACATGATTTTCTATAACATCAGTGGGTGAAGGAATATATGTCACATCGGTAGCATATATATCATTCATTTGGCCATCATAATCTCTATTGGCTAAGTTTTTGAATGTTGCATTAACATTCTTTATTTCTCTTTTTCTTTTGCTTCTTCTTATTTCGCATAGCAAACCCAATTTATTCATAATTCTGCCCAGAGTCCTTGGATTTATATCGATTTGGTACTTTTTCTTTTAAAAAAAGTGAAAGTCTTTCCCTTCCAAATCTTCCTTTGCATTCAATAAAAGCCTCTTTTATTTGTTTTTCATATTGAATTGCCTTGTTTTTGGCTGGCAATTTAAATTTTTTAATTCCTAAAATAGTGATAATTTTGCTTTATTGATATTGAAAAATTATGCTTCTTTTTAATTTTTTTCAACAACTTCTTCTAAATTTTTGCTATTGTTGCGTTTTAAAATTTCTTCATAAAAAATATCAGTAATGAGATATTCAAGTTGTTCTCTTGACATTTCTTTTAAAGTTTTTTCACGAAGTTTTTTTCTTGTTCAATATCTCTTTTTCTCGTGCGACTTGAGCCCTTACCTTTTTTTGGTAGCCTTGCCTGTTTGCGAATTTATATTCATACCTAAATTATAGAGTTTATATTTGGTTTTAATTAGTTTTAAACTATATTTATTTAGTTGCCTTCCATTACTAATTTCACAATATTTATATGATAAAAATTCTTTAGAAATATCATAATTTAAATATTGTTCATATCAAAAAAATATTGATAATCATTCATCTTCTCTTAATTGTCTTCCCATAATAATTTAACCTTTCAATAATAAAAATAAAGACTCGCTTTTTTTCATTACGAGTCTAATTTTTTTGTTCAGGTTTAAAAAATGGGCTTTTTTTATTTACTAAAATTTAAATTCTGAAAATTATCAGTTTTAAAATAATAAAAAATAGCTCAATTTTTTGAGCTATTTAATTTAAATTTTATTTTTTAACTAAAGTAATTATTTTCCAGTAGTTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGCTGGTTGTTCTTTACCTGGTTGTGTAGTTTCAAAGTTCACTTTTTCTGTTTGATAATTCAAATTATCAGATATTAACAAACCTGAAGTCTTGATTAATCCACTTTTACTAATAATAGTTAATTGTTCATTAGGTTTTGGTTCACGAGGTAATTTAACGTAAATTTTTTCACCAACTAAATGACCTTTTTCAAGTGTACTTTCTGTTTTGTTTTCAGCTGATGTAAGTGCAGCATTAAATTCAATGTTACTTAATGATTTTTTATCATCATTACTTAGATCTTTAAAGTTTTCAATTTCGTAAACCGCATAAAAACTTTTACCGTCTGTTGATTTAGCAAATTGGTTACTTAACTTAGATTTAACAGTTGAATTAGAACATGAAGCTGCTATAGCAACTATTCCAGCTCCAACTAAGGTAACACCTAATGTCATAGCTCAGAATTTTTTATTTTTTAATAATTTCATTTATGATTCTCCTAATATAAAAAATATGTCATTTTATTGTTTTTATTTAATATATGTAAAAAATATGAACACTAAGATTTGGTAATCTTACATAATTTCTACAGTAATTAATTTTTAATTTTAACGAAAACATATAAAGATTGCAAATACTTTTTATTTAGCACTTGCACTTTTAATTATATAATTTTTACTATTTTTTTTATTTTTTTATTTTTCTGCATTATCGATAACATTATTAGATATTTTTTATTATAAATTATGATTAAAAATTTAATTATATATCTATATTATTTTTAATAAGTTTTATTATTTGAATTATCAAACAGAAAAAGAATTAACAACTGCTAAAACTGATGTTGAAAGCAAACAAAAAGTTGCTGATGCTGCTAAAACAAAATTAGATGAAGCAACTAAAGAATCAAAAAACGCAAATCAATTATTAGAAACAGCTAAAACTAATCAAACAACAAAACAAGAAGCAGTTGATAAAGCTAAAGAAGAATTAGAAGAAGCTAAAAAATAAAAATTTATTAATAAATACCACTCAAAAATCTTAAAAACAAGATCTATTGATTGGTTTTTTTATTACTTTAAAACTGATAATTTTTAGAATTTGAATTTTAATAAATAAAAAAAGCCCATTTTTGAGCTATTTTTATTTATATTATTTTTGTGTTTCTATGTTAACATTTAACAATTTAGCATTGGTTAATTTAACATCATAATTTGCATTATTTCCATTCATTTTATCTTTTAAATCAATTTCTAAACCATTAAGATTTGATAATTGATTCTTTGTTAATGATTTATGAACTACTTGTAAACTACTATCTTTTTTAATTTCAATATCAAATGTTAATATAGGTTGATTTTCTTCTAAAGAATTTAAAATATCAAAATTTTGTAATTTAACATTAATTTTCTTATTTTCTTTGTTGAATGTCATAAATAATTTTGCATTTTTAACATATGATTTTAACAACTCTTCTTCATTAAATTTGACTTCTTTACCATTTAAAGTTAGTTTTGAAATTTCATAGTCAACATTTGAATTTAAACCACTTAGTTTACCGCTTAATGTTTTAGATGCTTCATCATATTTTAAATCACTTGCTTGAATTGGATTTGTATCAGCTTTCTTAATAACTTCTAAAACAAAATCTTTTTTATTAGCATCTGCTAATTCAAATTTACTAAATGTAACTTCAATAGTAGCAGTATTATTTTTGACTTCACTAATTTTTACACCACTAGCTACAACATCATCTTTTTTAGAATTTTCTGGTTGATCAGCTTGCTGTTCGCTACCACCATTTTCTTTTGGTTCTTCAGTTTTTGGAGCTGGAGTCGGAGATGGTTGTTGGCTTGGAGCTGAAGCTGCTTCAAAGTTCACTTTTTCTGTTTGATAATTCAAATTAAAAATATAAAATATCAAGTAATTTATATTTTAAAACATTTTTGAAACAAAATTTTGTTATATAAATACTTATATATTATAAAATAATAAAAATAAGATTATTCAAGTAATAAATGCAACTTAATATTATTGGTCTGCATTTATTTTTATAAATATAGGTGTTAAAATGGAAACAAAGAATGCATTATTTAAAAAGATTGTAAAAATTTCTGATCAATTATTAAATAAAATTAGTATCAAAAAATTAACTAAAGATAAAAAAAATAATTTATTTGTTTATTTTGACGAATTTGTTGATGTAACAATTATTGATGAATTACATCAATTATCAAAAACAACATTAATGCATGATTTACAAATTTGATATATTAATAATTTAAAAGAAGTTGATCATAAAAAAATATTAACTTTTTTTAAAAAAATTAGTGAACAAAATGCTAATTTAATCTTTTTAGAACAAATAAATGATTTAAATACAAAAATAGAATATAACTCTAATTTAAATTCATTAATTTTAAAAATTAATGATAAATTAATTTATGATCATTTTATAGCAAATAAATTAGAAATTTTAAACATCTTAAAAGTTTGATCACTACCTTATAGTAATTTTGAAATTCATTTTGAAAATCTTAGTTCATTATTAAATGAAAAGCATGAACAAGCAGTAAATGAAATTATTAGTCATCATATTCAACAACAAAAACATTTAGAACAACAAATTAGTCAGCAGCAAAATTTTTATAATAATCAAAAGGCAAATTTTAATTATTATAAAAATCCTTCAAATAAAACAATTACAAAATTAATTGATATTAATCCTTTAATGAATAATGCAAAAATTCGAGCTTATGTTTTTTTAAAAAAGATTGATATATTAAAAAGTGGAGCAATAGCTTATAAACTAAATGTTATTGATGATAGTGAAACCTTAACAATTATGACTTATTTACCAAGCGGTGAACATCCTTTAAAAAAGTTTTTAGATGAACTGAAAATTGATCAATTAATCGAGGCTGAAATTGATATTGTTTTAGATAATATGAGTAAGAGTGGTCAAGTTCCTATTGGTAAAATTAAAAAAATTTGTTGCGTTGAAGATAAACATGTAAAAAAACAAATCACACCTCGTTTAGAACTAAATTTTCATACAAAAATGTCATCACTTGATGCAATTATTTCGACACAAGAATTAATTGATTTTGCGGTTAAGAATCAATTAAAAACAATTGGCATCACTGATCGAAATGTTGTACAAGCATATCCTGAAATCGCTAAATTTTCTAAAAAACAAGATTTAAAAATTATTTATGGTTTAGAAACAGAAGAATTAGAAGATCAAATCCCATTAGTTTTAAATGTTCGGGATCAAAATTTAGATAATGCTACATATGTTATTTTTGATATTGAAACAACAGGATTATTTCCTAATTTTGATGAAATTATTGAATTTGGGGCCGTGATTATGCAAAATAATAAGCAAATTGGGGAAAAAATTCAATTTTTTATAAAACCTATTCAACAAATTAATGAGAATGTTACTAATCTAACTAATATTAGTCAAGAAATGGTCAATAATGCAATTGATGAAAAAACAGCATTATTAAAAATTAAAGAAATTTTTGATGACCATATTTTAGTTGCACATAATGGAATTAATTTTGATATTAATTTTATTAATCAGAGACTGTTAAAATGAGGATTAGAACCTCTTAAAAATCCTAGTATTGATACATTAATGATATCACGAGCAATAAATCCATTTAAAAGTCATCGATTAGGAGCGATTTGTAAAAAATATGAAGTTGATTATAATGATGAAAGTGCACACCGTGCAGACTATGATGCCATAGTTTTAGCTGATGTTTTTAAAGTTATGAAAAATAACTTATTTAATGATTTTGGAATTACTAATTTAAGTGAAATTAATACTAAATTACAAACAACAATGTTAAAAAATCGTAGTTTTGGTAATTGAATTAATCTTTATATTAAAAATCAAGCAAATGTTAAAGATATGTATGAATTAGTGTCTATATCTCATACTGATATGTATTATACAAGACCAACAATTACGACAAGCTTTTTAGCAAATAAAAAAGATAAATTAATTATTTCTAACTCGATTCACGAATCAGATTTAATCAATGCTTTATATTCAAAAAACGATGAAGAAATTAAAAGGTTAATTCAACGTTATGATTTTATAACATTACCATCATTAGGTTCACAAAAACACTTAGTATATGCTAAAAAAATTACAATTGAAAATGTACAAAAAGCTTTTAAAAAATTGATCTATTTAGCTTTAGAATTAAACAAGATTATTATTTATTCAAGCTCGCCTTATTATTTCTTTAAAGATGATAAAAAATTTTATGATGTTTATGTTAATACGAAAGGTCTCGAAGGTAAAGCACACCGATTTGCTAATGAAGTTTATGTTCCTGATTTAGAATATATTGATCAAAAAAATGCCATTGATGAATTAGCTTATTTAGAAGATGAAAAGTTAATTAACTTGATTATTAATGAGAATCCTGTGCATATTAATAGTTGATTTGATGATAGCATACAACCTTTAAAAGAAGGATTGTATGCTCCAAAAATGGAAGGTGTTGATCAAAAAACAATCGATTATGTTTATCATACTGCTAAAAAAATATATGGAGAGAATTTACCAACAATTGTTGAACAGCGCATTAAAAAAGAATTAAATTCAATTATTAAACATGGTTTTAGTGTTGTTTATTGAATTTCACATTTATTAGTAGAAAAATCAATGCAAGATGGTTATGGTGTTGGTAGTCGTGGTTCGGTTGGCTCATCACTTGTCGCAACGTTTTTAAATATTACTGACGTTAATCCGCTTACACCACATTATTTATGTCCTAATTGTAAAAAATGTGAATTTATAACAAACGCTGATGATGGATTTGATTTAGCTCCTAAAAGTTGTGAGCAGTGTCAAACTCCAATGTTAACTGATGGACATAATATTCCTTTTGAAACTTTTTTAGGTTTTGATGGGGATAAAGTACCAGATATTGATCTTAATTTTTCTGGAGTTTATCAAGCAGTTGCACATAATTTTATTAAAAGTATTTTTGGAGAAACACATTCTTATCGTGCTGGTACAATTGGTACTATGGCCCAAACAAGCGCTGAAAATACGGTTAAGAAGTATTTTGAAAACCGTTTTAATGAAAATAAAATTATTCGTGATTCAACGGTTAGTTTATATGTACAAAAGTGCATTGATTCTAAACGCACAACTGGTCAACATCCTGGGGGTATTATTATTGTTCCTAAAGAATATAGTATTTGGGATTTTTCACCTTATAATTTTCCAGCTAATGATATTAATGAAACTTGAAAAACAACTCATTTTGCGTTTGAATATCTACATGATAGTTTATTAAAATTTGATATTTTAGGACATGATAATCCAACAATTTTAAAACTTTTAAAAGATTATACGGGGATTGATGAACGTGATGTACCAATGTATGATCCATTAGTTATGAAATCATTTAGCGATATTAGTGCGCTAAATATTAAACCGTCTGATGTTTTAAATGAAACAACAGGAGCAATTTCTATTCCTGAATTTGGGACACGCTTTGTACGTGGTATGCTAGTGGATACTAAACCAAAATCATTTGCTGACTTAATTCGTATTTCAGGATTATCACATGGAGAAAGTGTTTGATTAGGAAATGCACAGTCATTAATTAAATCTGGTAAATTGCTAAAGGATGTTATTGCTTGTCGTGATGATATTATGACATATTTAATTCGTCAAAATGTTGAACCAAAAACTGCCTTTTTAATTATGGAAGATGTTAGAAAAGGTAAAAAGATCAAACCAGAACACCAAATCATTTTAAAAGAATTAAAGGTTCCTGAATGATACATTGAATCAGCTAATAAAATTAAATATATGTTTCCCAAAGCACATGCCACAGCTTATGTTATGCATGCATGAAAATTTGCTTGATATAAAATTTATTATCCACTAGAATACTATGCAGCATTTTTTAGTGTTCGTGCTGATAATTTTGATTTATTTGTAATTAATCAAGGTAAAGAATTTATTGAAAAAACTTATAATGATATCGAACAACGTTCTAAATCACGCGATCCACAAAAAAAAGTTAGTTCACGTGAACTTGCTTTACAACCAATTTATGAAATTGTGATTGAATTATTAGCTCGAGGTTTTAAAATTTCAAATATTAGTATTGAACAATCACAGGCGACTAGTTATGTAATTGATAAAGAAAACAATGCTATTATTCCACCATTTATTGCGATTCAAGGATTGGGTGAAACAGTAGCTAATTCAATCATTGAAGCTCGTAATCAAAAAGTTTTTTCAACAATTGAGGATTTAAAAAACCGTACAAAAATTTCGCGTACAGATTTAAAAAATTTACGAGTATTAGGCGTTTTAGATCATTTAAGTGAAACTGAACAACTAACATTATTTTAATTTTTTTGTTATATAAAAACCCTTTCTTTATTTTTTTAGTAGTATAAATTTAACTGTGTTTATTTTTTAAACCAAATTATTTTCATTAAGGATAAATAAGAAAGTAGATATAAAATGAAAAAAATAAATAAAAAGATTTTGTTTTCTTCATTGTTATTTGGAACTGTAGCTATTGGAACTGTTGCAGTTGCAACTGCATGTAGTGACAATAAGAAAACTAAAAAAACAATCAACACAGGCACTACTCAACCCGGAAGTAGTACAGGAACAACAAGTAAATCATTAACGCAAGAAAAAGTGTTAAGAAATGAAATCATTTCTGAAATTTTGAATGCTAAATCTAAAAAACCAGATCGTGATAAGATGTGAGTTAATTGATGAAATAGTTCTTTTGAACAAGCACGAAAACTAGCAAGGGAAATGGATAAAGCAGTTGATTTAACAAAATCTTCAAATTTTAAAAAATTAATTGCTGATGGAAAATACAAATCTACATTTAGTGCACAAATTGAAGATATTATTACTAAAGTTAAACATGATATTAAAATGTATGCAGAATCACCATTTTGAAAAAAATGACGTGATGAAAAAAAGACTGTTATTACATTAGTAAATAACAACGCTCCTCGTGAAGATTTAGGAGCTATGAATGTAACATCAATTGATTTACCTGCTGATTTTCCAATTATTTATTCAAAACCTGATTATGATGGTATTCCTGGATTAGGTGCACGTTTTCCAACACCTAAATCAAAAGTAGCTCCTGAAAAATTATTAGATGATGGTTTCTCATTTGGAGATATTATTGTTGAAGATGGTTCAGATACTCAAAAAGCAAATCTACCTGGTCAATTAATTGAATCATTTGAAAAGACAGCTGATAAAGTAATTTACTTATACTATGATTCAGGTTTACCAGAAGCGTTTAAAAACAATCAACGTCAACCTGAAAAAATTAAACAATTTGAAGACTGAATGAAGAGTCAAAATGCTAATGATTTCGTTGCAAAACGCATGTTAAAAAATCCAAATAATAAGGACGATTTAATTGTTATGCCAATGAGTAGTTTATGATATGCAAGTTATGGTATTTTAGGAGTTAATTATTCATTACATGCCCTTTCAGAAGCATTTGGTATGCCAAAATCTGAATTAGATGCTTTAAAAGCTAAAGAAGAATTTAAAGTGCCAAAACAATTAGTTACATTAGTTAACCAAGCTACAGATTTAAAAGAAGATAAACAAACAATTAAAGATGAATGCGATCCTTTTAAATCACACCGTGATCCAAATCATAAAATTGACTGAAAAGTTTGAGCAACAAATTCACAAGTTTTAGATATTGCTATTACTTTAGGACTAAAACCTGATTTATTAGTAAATGGTGAATTATCAACAAGTGGACATGAAGAAAATCAATTAGCTTTATATTTATCAGATTATATTAATGGACAACTAAAAGATTGTAGAACAATTACTCCAAATGATGGAATTCGTTGAGAAACAACATCATTAACTAAAATTAAAGATTTAAATGTTAATTTGATTCTAGCTGGAATTCATGGGGAAGCTGCAACAAAAATGTTTGGTGCATTAATGAAAGAACATAAAGAAATTGCCAATTTTGCAATTACTAACCGCCGATTTAATGATGAAACAAGAAAAGTAGTTGCTCCTCAAGATCGCGATAAATACTCTCAAAATGCAGCTCTTGTTGATTGAGAAGATTACTTAAAAACAAAAGATTTACATTAATTTTTAATTAATAAAGATAAAGATTATAATACAATAAAAAATCCGTCTTATTATGTTTATAACAAAGATGGATTTTTTATTATTTATTTAATTTTTGTAAATAAAAATCAGCTTTTTGATAAGCATTTTCGCCATATATCTTAATTTTTAATTCATTAGTTTTTAAAAAAGCGTTTGTAGCTCATAAAAAACCATAATATGATGTTAAAAATAAGTATTTATAAATCAAGCATTCGTCATATCCTAATTTTTCTTTTATAAAAGAAATTCCAAAATTTAATTGGACATCATTGAGATCTCTTAAAAAATTAAATAAATCTCAATCTTTTAAATTTCTACTACTATATTCAAAATCAATTAATATAAAAAGATTTTCGCATTCATTTGCTAAAATGTTATTAATTGAAAAATCATTGTGAGCCAAAACAAGTTTAGTTCTTTTTACTTCTTGTTCATAAATAGATTTAAAATATGAAGCTAATGGATCATCTTTTACCAAAGGTAAATAAGATAAGTAATCAAAATCTTTAATTTTAATTTTTTTGTTAATGGTTAACGTTTGTAATTCTTTGATATTATGAATCACGTTAGCCATAAATCATTCATTAATTACATAAGGGTTTTTTGACATTTATTTTTTGACTAATTAGGTGATGAGATTGTTGATCATAATAAAAATAACCTTTGAAAAATTTAGTAAACAAATAATGCTCTAAAGTATTTCCCTTTTTGATTTTAATTCTAATATATCAACTTAAATTGATTATGTCTACAACTTCAAATACCTGATTAGTTCAAGCATCTAAAACAATTTGAATCGATTTAAAATTATGAAAATCAATATTGATAAAATCAATTAATAATGATTTTGCTCAATTTTTCATTAATTTTGATTTTTAAAATATTTTATTAATTCATTAACAAATTGATTTAAACGCATTTGATCTTGATTAATTGCACCATAATAATAAAAACTTTTTTGAATACTTGCTCGTAATTTTTTCAGCATTCCATTTACATTATTCAAATATTCTTCAACTGAAATATTATTAATATTAGGATCTTTTTTTTCATAAAAATCTTGATTAGCTCCAGTTGTGATGATTTTATAAACTTCTTTATTTTCTAAACTAAAAGGAAAAGTTCTTCAAACTTCAATAAAATAACGTGATAATGATCAAGGAATGTTATATCAATTTAGCGTAAACAATAAAATAATTCGATCAAACCCTAAAATAAATTCTTGTTCTTTTGAAACATCAAAAGGTTGATTTGCATCAAGATTAACACAAACAAAATTTGTTTGTTTTTGAAAAGCTTCAAAAATTTGTTGTGTATGAAACGATTTAGTTGGACAAACGCGTGCGTATAAAACTAATGTTTTTTTCATGTTATTTAACTCCTTAAATTTATTTATTAAATTAAAACATAATTTAATAAAAATTAATATCATTAATTATTAATAATGATGAAAAAAACTATATAAAATATTTATCTTATCTTCTAAAGAAAACTCTTTTTGATAAGGGATTTGAAGGAATAATATATAAAAAAGAATTATATTTTATTTAGTTTTTCACGTAAATTAACTAAGACATTTTTAGGTAAAAATTGTAATAATTCTTGATCAGATGCACTTTTTAAATCATTAATGCTATCATAATATTTTAAAAGTTCATTAAAACGTTTTTTACCTAGACCTTGAACATTATCTAATAAAGAATTAAAAATAGATTTTGTGTGTTTATCTCTTAAAAACGAAATTGCATAATTGTGAACATCATTTTGCATATTTGCTAAGAATTTATATAAAATACTTTTACGATCTAAAACAATTTCATCGCCATTAGTTAAAACAATGGCATCAGTTTTGTGTTTTTGATCTTTTTTTAGTCCTATTAATGGAATGTTTATTTGTAACTGTGCTAACGCATATAAAGCTGCATTAACTTGTAACTTTCCCCCATCTACTACAATTAGATTAGGTAATTTTTGATGTTGTGAAACTAATCGTTGATAACGTCGTGTGATAACTTCAATCATAAAATGATAATCAGAAGCATTTTTAGTTGTCATTAAATTATATTTACGATTTAATTTGTTATTTAATTTACCGTTTTGATAAACAATCATCCCAGCAATTGGTAAACTTAAATTAATATTAGAATTATCAAAACACTCAATGATATTTAAATCTGGTAATTTTAAAATTTGTTTTAATTGATTTAAAGCATCATCTAATTCATTTTGTTTATTAATTACATTTTTAATATTAGATTCAAAATACAAAATAGCATTATCAAGAGCTAATTTAAGAATTCGTTTATGAAATTTTGTTTGCGCATTAATAAATTTTGTGTGAAAACGTTGTGATAAAGCTAATAAATTATCATCATCTAAACTTACAATAATTTTTGAACTAATTTTATTTTGTGAATAGTATTGCATTAAAAATGAGGTTATTAATTCATTAATTTCATAATCATAAATGGCAAATGTATCAGCACTTTTATTTAATAATTTGCCTTTAACATAATTAAAAATTATAATTACTAAATAATTATTTTTTTCATAAAAACCGATGATATCGAAACTTTCATTATTATCCAATTGCACTAATCCTGAATTAATAATTGAAGTAAGTGCTTTTTGTTGCTCAGCGTATTTTTGAGCATTTTCAAAATCTAATTTAGTAACTGCATGTTGTTCTTTAGTTTGTAGAATCATTAAAATGTGATCTAAATTACCTTTAAAGATTTCTTTAACTTCACTGATTGCTTCTTGATATGTTGATTGTTGAACTTGATTAATACATTGATGCAGGTCATAATAAATACATTTTTTATATGGTTGATGATTACATTTATTAAATGGAAAAATACTATTTAATAAATTAAATAATTGATATTTATTTGTGCTATCAGCTAATGGCCCAAAATAAATTCCTTTATTTTTATCATAAGTACGAACATATTTTAAACGGGGATATTGTTCATTTGTAATCATGATATAAGGATAGTTTGAACCATCTTTTAATAAAATATTATATTTTGGAAGATGTGTTTTAATTAAATTGTTTTCTAAGATTAAAGCTTCGTTAACATTATTAACGACAATATATTCAAGATCATTAATGTTATTAACTAATTTACTTGTTTTATTGTCTTTACAACCATTAAAATATGAATGGGTTCGATTATATAAATCTTTCGCTTTACCAATGTAAATAATTTGATTGAATTGATCTTTTCATAAATAACAACCAGGTTTGTGAGGAATCAGTTTTAGTTTATTTTTTAATAAATCATTCATTAACAAAAACTTTTATCAAATAAATCCTGGGGATTTAAGGCGTTAATAATTACCTTATATAAATTTTGGAAAAGTGGAGCACTAATTTGATTTGTTTTAATAATTTCATAAATAACTTTGATGTTATTTAAACCTTCAATAGTCTGTTGTTTATTTACTAAAGCTTCTTGTACGCCTTGTTGTGCAATCAGTAAACCAAAACTAAAATTACGTGATTTTTGATCAGTACAAGTTAAAATTGTATCACCAATAAAAAAGTATTCTAACGGATTGACTTTTTTGTGAAAAATTTTCTCTAAAATTAGTTGCATTTCATTGATTCCTTTAGTTAAAAAAGCTACTAAAGAATTATTTGAATTTTGTAATCCATAAATGATACCACAACCTATTGCTAATGCATTTTTAAAACAAGAAATATAATTAGCAGCATCAAACTGTTTAGATAAAATGCATTTAAATCAATTGTTTTCAAATTCTTCTTTAACTTTTATAAGGGCGTTTGAATTATTGCCTACTAAATTAACGACTGTTGGTTTTTTTAAAAAAACATCAATTGCAAATGAAGGGCCAACTAATCCAACTAAGGCTTTAATTTTTAAATTTTGTTTAATATAATCACACCATGATGATTTTGTTTCAAGATTTAATCCTTTCGCTGCATTAACAACAATAGTTTTTGATAAATTAAAAATTTTGATATTATCAACAAAGTTTTTAATAGCAAATGAAGGAATAGCAATGATGATAAAATCATATTGATTCTGAAGTGCTAAATTAATATCCAAATATGTATTATTAATTGGTAAATCTAATTTTTGATCCTGAAAATATTTCTTATTTTTTTGATTTTCTTTCAAATCATTAAGCTCATTTTCATTAATTCCATAAACATCAACAATATGAGAATTTGAAACTAAAACTTGTGTTAAAGCACTTCCAAAAGCTCCGCTTCCAACAATTAAAATTTTACTCATTATTATTTTATTCTCCTCGTAATTTTTTATTTTTATCTTGTCAATATAATGTTATTGGTACACTATCAAATCCAAAAGCTTTACGAATTTCATTTTCAATATATCGAGCATAGCTAAAGTGCAAAAATTTAGGGTTATTACATTTTAAAACAAAAGTAGGGATTTGTGATTGTACTTGAACGATATATGAAATACTGATTCTCCCTCCTTTAAATGGTGGTGGTTCTTGAAAAGCATTTGCTTTAATAACAACATCATTTAATAATGAAGTTGCAATTTTGCGCATTGCTTGCTCACGAACGAATTCAATTGCTTCAAAAATAGTGTGAATTCTTTTATTGTCTAAAGCACTAACAAAAACAATTGGTGCTCATGATAAATACTTAAATTGTGAACGAATCTGTTTTTTTACCATTTCCATTGTATGACTATTTTTGTTAATAATATTATCTCATTTATTAACAATAATAATTGTTGGAATATTTGCATTATATGCTAAACCACCAACAACTTCATCTTGTTCTTTAAAAGGTTCAGATCCATCAAGTACTAATAAAATTAATTGGGATCTTTCAATTGCTTTTTTAGTTCGTAATACAGCATATTTTTCAACAGATTCGACAATTTTACCTTTTCGTCGTATTCCTGCAGTGTCAATGATTGTATATTTTTTATTATAGTAATTAAAATCATTATCGATTGAATCACGTGTTGAACCTGCTTCAGCATTAACAATTACTCTTTGCTCACCTAAGATGGTATTTGTTAGTGAACTTTTACCAACATTTGGTCTTCCAATAATGCAAAATTTAAAGCGTTCTTGTTTTTCTAAATTATTTTGTATAGGCATATCTTTTACTAATTCATCTAAAAGATCACCCATTCCTATTCCATGCTCAGCTGAGATTTTAAAAAATTTACCAAACCCAAACGAATATAGTTCTCCTTCATTAAGATAATATTTTTCTGATTCGATCTTATTTATAACTAAAATAATTTTTTTATCTTTAGCTTTTTCTTTTAACATTTTAGCTATTTTTTTATCATCATTATTAATTCCATCTTTTGCTGAGACTAAAAAAATAATTGTATTTGCTTCATTAATCGCAAATAAAACTTGTTCATTGATGTTATCTTGATAAGTATCTTTTGAGCTAATTATACCTCCTGTATCAATCAACATAAAGCTTCGCGTTAATCAATTACCAATATCATATATTCGATCTCTTGTTACTCCTGGCTGATCGTCAACAATTGATTTACGGCGCATTAAAATACGATTAAATAATGATGATTTACCAACATTTGGCTTTCCGACAATAGCAATAGTTCGCATAGATTATTCCTTTTTAATCACAAACATTATATGTAATTATAATAAAAAACAATTTAATAATGTTTGATTAAGCTTTCATATTTTGAATATAAATTATTTCTTTATTTGGCAAACATTTAAATAAAGGGTGGTTTTGTGATTAATAATTTATTTAAAACTAAAAATTATTATATTAGTTTTTTAATTTTAGCAACTAGTATTGCTTGCTGTTTCTATTATAATAATTTTTGAATTTTAATAGTTAATATTTGTCAATTTTATTGATTATATTCAATTAAATTTAATTATAAAATTATCATTTTAATCATTATATTCAGCGCTTTATGTATTATTTTGTGTTTATTAATTCACAAAGATTTTAATATACATTTTTTAGTTATAAATATCGAAAAAAAATATAATTACTCGTTACGAACACAAATTTTAATATATCTTAGTAAAAAGTACTCAAATGAAAATACTTTAATGTTAATTAAATTAATGATTTTTAATGAAAAGATTAACAGTGATTTTAAGCAAATTTTATATGATCTTAATATCGCTCATTTATTTATTGTTTCAGGATTGCACTTGAATTTATTTATTCTAATCATTAATAAGATTTTTTCTAAACGATGACCCAAAACAAGTTTTTTAATAGGAATATTATTTTTACTTTTTTATGGATATTTACTTGAATTTTCAATTGGTTTTATTCGAGTTTTTATTATGCATATTTTATCAATTAAATTTTTTCAAAAAATTTCTAAAATGGATAAATTGGCAATTAGCGGAATGTTAATTGGTTGTTTAAGTATTTATAATTTATCGATTTTGAGTTTTATTTTTGCATATTTAGCTTTATTTTGCATTTACCTTATTAATATTTTTATAAAGAACAATTCGGTATTAAAAAATATTTACATAAATTTGTATATTTTATCAATCACTTTTGTCATTTCAATCAATTTAAACCAAAAAATCAATTTTTTAAGTATTGTTTTTGGTTATGTAATGAATTTACCAATTCTTTTTGTATATCAAATACTTTTTTGATTAATGTTTATTCCTCATTTTGAATTAATTTTAGATTTATTTACTAATACATTAGTAAAATCTATTTATTTTATTAGTGAAATTGATGCGAGTATTCTAATTAAAGCTTACGTAAAAATTGTTACGATTTTTTATACGACTATTTGAATAACAACTTCTCTTTGAATTTATTATAAAAAAATATTAAAAAAAGTATAAGAGTATACTATTTTTCAAATTCGTGATATATAATATAGTTGCCAAGTGTAAAAAAGTGGGGAAAAATGTATAAAAATGTTTATTGGTACTTATAATCATTCAATTGATTCAAAGAATCGTATGTTAGTTCCATCAAAAGTTAAAGCAACATTAGGTGAAGCCATTTTTGTTTATTTATCACTTGGTTTTGATGGAAATATAGATATGAGATTAGAGAGTGAATTTAATCAATTCGTTAACAATATTAATAATTTATTAATTGGTTCAAAAGAAGCGCGAAATCTTACAAGATTAATATTAAGCCAAACTTATAAAATAGAAATCGATAGTGCGTCAAGAATTTTAATACCTCAAAACCTTATTGATAAAGCTAAAATTAAAAAAGATATTTATATTATTGGAACAAATGATCGATATGAAATTTGGGCTAAAGAAGTTTATGATGATTTTAGCTTAAATCAAGAAAGTACGCTAAGTGATTTAGCTGAAAAATTATTAATTAATGGAATTTAATCAACACATTACCGTTTTATTAAACGAAACAATCGAATTATTAAATATTAAACCAGATGGCATTTATGTTGATTGTACATTTGGTCGTGGCGGACATTCACAACTCATCTTAAAAAAACTCTCAAAAAAAGGTAAATTAATTTGTTTAGATCAAGATCAAGAAGCAATTAATTTTGCAAATAATTTATTTAAAAATAATACAAATGTAATTGTTATTAAAACAAACTTTAAAAATTTAAAATCAGCATTAAGTGCACATAAAATTTTTTATGTTGATGGCTTTATCTTTGATTTAGGTTTATCAAGTCCACAACTTGATGATCCAAAAAGAGGTTTTAGTTATCATAAGAATGCTTGATTAGATATGCGAATGGATCAATCGCAAAACTTAAATGCTCACTATATTGTTAATAATTATTCTTTTGCAAAATTAGTAAGTATTTTTAAACGATATGGAGAAATAAAATATCCAAAAATTATTGCTGATGCAATTGTTAAAGAACGCAGTATTAAAGAGATTAATACAACATTGGAATTAGTTGAAATCATTAAAAAATATTCTCCTAAAAAAAATTTATTTGAAAAAAAACATCCTGCACGTTTATTTTTTCAAGCAATTAGAATAGAAGTTAATGATGAATTAAACATTTTAGAAAAAGCATTTAATGATGCTATTTCAATGTTAAATCCCTTAGGTGTTGTGGCAATTATTAGCTTTCATAGTCTTGAAGATAAAATTGTTAAAAAGGTCTTTAATAATTATGCAAAAATTAAATTACCTAAAGAAGTCCCAATAAATAATTATGTAAATAAATATAGTTTATTGAATCAAAAAATTATGCCAAGCACCCAGGAACTAAATGATAACAATCGTTCACGATCATCTATTTTAAGGGGATTAATTAGAAATTATTAAAAATGGAGGATTTTATTTATGAACAAACCAATGTGTTTGCTTCGTTTAGATAATTATTATTTTACATTGTTGGTATTTATTTATAATGATGTAACTAAAGTTAATGATTTAATTTTTATAAAATCTATTCCAAACAATAATCAAAATAATTATGAATCATCAAATTTTTTGCAAACAGATTTAAAAATCCAACTTAATACATTGATTAACTGTTTTAAAAAAGAATTGCACATTAATTTTATTGATGAAATTATTGTTAGTTATGGTTTTAGTAGCTTTCAAACTACACATACTACTTTTTATAACTATAATGAATTTATGAAATTAAGATCAAATTTTAATAATGAACACAGTGAATTATTAAGTTCAGATCAAAAAAGTTATATAGCAGTTGATTTTGGAATAAGCCACTATTCTAAAGAAAATAGTTATAAGTTGATTTCATATTATATTTTGAAATATGATTATCAATTAATAATTGATTTGTGCCAATCATTTAATTTAAAAATTAAGCAATTTATAGTTTCACAAGCTCATTTAAACGGTTTAAAATTTGATAATGAAGATAATAAAACTTCATTATTATTAGAATTAGAAAATTTTAAAATAAATTTTAGAACTTATAATGAATATAATTTAATTAGTAATGTTATTAGTGAGACAAAAAACCACTTTTGCCTGGCTGATATCATTACTCAAACAGCACAATCATTAAATATTAATCCTAAAAAGATTGAGAGCTACTTTAATGATATTAATTATAATAATAAAAAAGTTATTGAAATTAATATTGATCCTAAAGAGTTGTTTACTTATAGTTTTATTTGTGGTGCGGATATTGAAAATACTTTTTTAAAAATTTTTAAAGAACATTTTGAAAAAAGTATTTTATCTCAATTTGATCATCCATTACTAGATTTTAATCCAAAGAATTTAGCCTTTCTTTTTATTAACACTCCTTTCAATAATTTAAATAAGATATTTTATTATTTTTTTAGTTCATATTTTAAAGAAGTTAAAATTGAAGTTTTTCAAAATAAATTTTTTATTTATTCAAATTGTTATGAATATAGTAATAGTCAAGCCCTAGCTAAGCAAGATTTTTACAATGTACATAACATGAAAAATTTTGATAGCTTTAGCAATGAAAATATCCTAACAAAGATGCATGATCAAACAATAAACATAAAAAATTTTTAATAGAAATATAAAAAGTATACAATTATTAAAAAAACTCATTTTTATGAGTTTTTTGTTTTTATAATATATGAAAGATAGGATGTGATTTTGTTTATATGGCGTTAAGTAAACACAGTATTGGTTTGATTAAAAATTTAAAGAATAAAGGATTGACTATTAAAAATAAAGAAAAATTCATTAGTTTCTTGTTTAATTATGGAGTCATAAGAGTTATAGATGGTTATTCATTTTTTTTAAAAAAAGATAATCAATATGTAAAAGGCTTAAAAAGTAAGGATTTAATTAATTTTGTTTTATTAGATCGTAAAATTGCTAATTTAATGGGTGATTGTATTATGCTATTTGAACAACAATTAAATGCTATTGTTGTTCAAAATTTTTTAAAGATCAATAATTTAAATTCAAAATATGTTATAGATATGCGAACAGATCCTTTTATAAAATTTGAATCACCAAAACATTATGAAATATTATCAGAAAATATTTACACATATGCTAATTCTTCTAATTTGTTAAGTCAATTTAATACTAACGCACAAGAAATTCCTTTAATTAATTTAGCATTATCATGAACATTAAATACCACTATTGGCTTTTATCGTGCAGTTGATAATAATGTTCAAATTAGTGTTTTAGAGTATTTTCAATTAAATCATATTACACCATTAGAATTTATTGATATTTTACATATCATAAAACATTTTCGTAATAGTATTTCTCATAACGATATGGTAATTACTAATAGTTTCAATGATAACGTAGGTGCATTTGCTAAATCATTAAAATTAAAAAGTATTAAAAAAGTAGTGGGATTTTTAGATGTTGCGGCTTTTTTAACGGCGTTTACAAACGATAAATTTCATGTTTTAAATGGTAATTTACACATCAAAATTTTACAAGCAATTAAAAAAGCAAAATTCAAAGGCGTTGTTTTAGAACATATTAATAATTTATTAGGAGTTGAAGATTAAAATTATGCAAGATCAAGGAGTAAATAACAAGGCAATTAAGCAAAAAGTCTTAAATTTACTATCAACTGATACGCGTGATAGTTGTATAAGAACACGCTTAACAAAATACCATTTAGATATTAGTCACACTTTTAATAAATATGAACTTCAAGATTTTATTGAAGGTAAAATTAACATTCTTAAAATTAGAAATTTTATTAACATCAATACGTTTGAAAATGATGTTAAAAATATAAATTCAATGGATGAATTGATTGATTATTTACAGGATTTCAATTTAAAACTAAAAGATCTTTTATCGACACGTAAAATTAATGAATGGAGCCAAGACTTTGTATTTTATAAAAATGAACGAATTGCTGATATGATTAAAGCGATTGAAAGTCAACCAAAAAAATTTGAATATTTAAGTCGTGAAGCACGCAATATTTATGCGCAAACAGGTGTTTGACCTTTATATATAGCTAAAAATTTTTTGATTGGTACAACACCTAAAGCTTCAAATATTAACGCGCCCTTAATTTTTGTTGATGTTGAGATTAAAAAATATAATGGTGAAATTTTTTTAAAACGAAAAAATACTAATTTTATTGTTAACGAAAAAATACTATCTTTTTTAAAAAATGATTATAAAAATAATATCGTTTCTTTAAATGAAATTAAAAACTTTAGTTATTTAGAAATTCAAACTCTAATTAAAAAAATAATACTAAAAGATCATTTAGAATCGATTATTAATAATAATGAATTTCTAAACTTAAAACAAAATGAAATTAATGAGCAATTTAATAACTTTTTTTTATATGATGCATATGTTTTAGGAATTTTTGAGCCCCATGGAGGAGCGATTAAAAATGACTTAGAAGTGATTATTAGTAATAATATTGATCCTTTTGACGAAGAAATAAATAATGTTAATCAACAAATTTATTATAAAAACTATCATTTTTTAGCAGAAGAAAATAAGCTTCCTTTAATCCAAATTAATCGTCCTTTAAATATTTTTCAAAAGTATGCTATTCATTCAGGATTAGTTAAAAATACTTTAATATATGGACCGCCTGGAACGGGAAAATCAGAGGTGATTGCAAGTATTATTGCAAACGTAATTAATGATTTAAAATCGATTTTAATTATTTCGGAGAAAAAAGCTGCTTTAGATGTATTACATGAAAGATTAGCTAAAATTAATTTTTTCACACTTTTTATTTATGATACAAATAATGATGATCAATTTTATAATAACATTTTTAATTTAATTGAAAAAATAGGAGGATTGCGTTTTATTGAGAATCAAGATTTAAACCAATTCAATGATTTTAAATATAAAAATAATATTATTTCAAACATTTATTATAAAAAGATTCATGAATTAAAAAAAGAAATTATTAATATTACATCTTTAATTAAAAATTTACATAATGAAAAAGATAATAATGATAATAATTTTGCTAATTATTTGTATTGATTATCACGCTTTAATGAAGTAGAAATTAGATTTATTAACGATCCATTATTGATCAATGAAATTAATAATTTAATTGAGAATTATTCTTATAATTTTGATGAAATTATTACACACGTTAAAGCTAGTTATGATTTTTTTAAAAAATATAATTATGAATTTTCTAAAAATAATTTTTTAAAATTTAAAGAAGAAAGCGAACAATTAAATAAATTACAAATTGATTTTAATTTAATTACAAAATCTTTATATCAATCACAAACTTTTCTACCACGTATTAAGTTGTTAGAAAGCTTTTTAGTTAAAAATAATATGACTTTAACAAGTCTTATATATGATGATGTTAACTTGTTAAGTAATACATATGTTGAAGCTAATGAGTTTTATACAAACTTTAATCATCTTTTAAAAGATGATTTTAAAAAATTTATTGAAGATAATATAAAAAGTCATTCATTGTTTTTTAGTCATTTAAATACTTTAAAAAATAATGAATGAACTTATGCTTTTGAGCAATATTTATTAATAAATAATCCATTTGCTAAAAAAGGTTTATTTAAAAAAATTAAAGGTGATGAAAAAAAATTATGAAAACAACTTGATCTACTTAAAATATATATTAATAATCCTTTTTTATCAAAATTTAGCTTTGATTTTTTAGAAGATTTAATCAAACAACCAATAGAGATTTTCAATGTTGAAAATCTTGTTTATTTAAATAACAAACATTTATTAAATAAAGCATACGTTAAAGATTTATATGAGAATGCTTTTGATAAGAATTTATCATATGAACTAATTTATGGCCTTAAAGATTTAAAATTAAATAGTGAGCAATATGATATTATTAAAAATTTAATTAAATATCAAAACGAAGTTTTAGTTAATTATCAATTCATTAAAAATACTGATTTTTTTAATTTGTGTAAAAAATTAAGAGATGCAAATATTCAATTTTCTAAAAATGCTGATGAAATTATTTACAATAACTATTTAGATTATTTGCAAATTAAATTAGTTACTGCTCCTAAACAAATCCAAACAAAAATTAAAGAAATTGCGCAAATTTGTCGTTTAGCAAAAAAACCTAATATTGTGAAATTTATTTTAAAATATTATGAAATTTTACGTTTTCTTTTCCCTATTTGAATTAGTAAACCAGAACTTGTTAGTGAGATGTTACCATTAATAAGTAAGAGTTTTGATTATGGAATTTTTGATGAGGCTTCTCAGATGTTTTTAGAACGTTCTTATCCTTCAATCTATCGTTGTAATATTAATATTGTTGCAGGCGATGATAAACAATTATGCCCTACTAATTTTTTTATTAATCGTCAAGAAAATGAAAACGATGAGTTTGAATTAGCAGATGTAGATGTTGCAGAAAGTTTATTAGATCGTGCTAAAACTGCTCTATGGTCAGAATATTTATTAAAAAACCACTATCGTTCTAATCGAGAAGATTTAATTAGTTTTAGTAACGATAATATTTATAATGGCGATTTACATTTTGCATCTAAAAATGGGTTATTTGATCCAGGAATGGAAATTATAGATGTTGAAGGTGTATTTGAAAATGAAAATGTTATTGAAGCACAAAAAATTATGGAGGTTTTAAAAGAACGAGCAGCCAATTATCAAAAAATTATTGTTATAACTTTTAATATCAAACAAAGTGAATTAATTGAAAATTTAATACTACAAACTTTTAGTTTTAATGATGTTGTTTATCAACGTTTTGAGAATGATGAAATTATTGTTACTAATTTAGAAAACGTTCAAGGTAATGAAGCTGATTTAGTAATTTTATCGGTTACTTATGCCAAAAATAAAGAAGGAATTTTACGCAATAATTATGGCCCATTAATGAAAAAAGGTGGTGCTAATCGGTTAAATGTAGCGATTACAAGAGCAGCAGATAAAATGATTGTTGTTAAATCAATTAAAGCTGTGGATATGATGGTTAATATTAATAATGATAATATTAGGACTTTTAAAAATTTTATTGGTTATTGTGATGAAATTGTTCATTTGACTAATCAAAATAAATTTAATGATTGTAGTCATTTTGATGAAGAATTTTTAACAAGTTTAGAAAAAACCATATTGAAAATTTTAAAAGATTATAAAAAAACAAATCAACAAATTTTATATAAACTAAAAATTGGTAATTTTAAAATCACTTTTGCTTTATATAATGAAGAAAAACAAAAAATCGATTTACTTATTTTTATAGAAGAGTTTAATGAATGAAAATCATATAATCTTTTAGAAATTTATGATCAATTTAACTTCTTAATGGATCGTGGTTATAAAACAATCGTTATTAATGATTATGAAATTTGTTTAAACTATAATCAAGTTAAAAATAATATTATTAAATTAATTGATTAATATATATTATTAAATTGTTAAAATTTATGAAATATTTATTATCTTTTCTGGAGGTTTAATTTGAATTTAAAGTCATTTGAATTTTTAAAAGGTGATGCAATTGTTGATAACCAAATAAAAAAAGCTATTAATACTAGTTTGATTAAAGCATCATTTTCATGTGCAATTATGTGTATATTAGTTCTTATTTTTGGTTTTTTAATGCGAAAATATTTATTTTATTTTTCAGATGCATCTAGTTTAATTATTAGCTCTCGTTTGATTTTTCAAATAATTACAATCATTGTTGCTACTTTATCAATTGTCTATTTTATTGTTCATTTAATCATTTACTTTAGACAAAGAAATAATTTTTTAGCTCCTAAATTTGCATGATCAATCATATTGTATCTATTGGATGGTATTACAATGTGTTTTATGATTAGTTATGTAGCGGCGATTGTAGGATTAGATTTGACGTTAATAGCCATTGGAATTAGTTTATCAATCATTGTCGCAATGGGAATACTAGGTTTTATTATGAATCATAAAATTGCTTTTAAATTAGGCATTGCTAATATCGTTTTATTTACTATTGCTACAATTGGTTCAATTATTTTATTGATTGTATTTTTTACAACGAGATATAACCATACTGGTTTTAACAAAGGTGTTATTATTGCTGACATTGTATTAAGTATTATTTGATTGCTCGCTTTAAGTATTGGTTTTGCTTCGACGATTTACATTATTAAAGTAATGGCCGAACATCATAATTTAGATAATAAAAAAATAATGCGTGATTTTACAACTTGAAATAGTTATTTACTATTTGCTTCATTCAATCGTATTTTATTATATGTAATTCGTTTGTTTTTATTGTTTAAAAGAGTATAAATATAACAGGTTTGTTTAAACCTGTTTTTTTATGACAATACATTTATTTTTTAGCACTCATTATTTTAAAGTGCTAAAATTATAATAGTAAAGTAGGTGATTATTTATGGAATTTAAACAAAAAAACTCAAACAATCCATTAGAGGAATTTGGACGTAATTTAAATAAGGAAATTTTAGATAATAAAATTGACCCCATTATCGGTCGAGATGAAGAAATTAGAAGAACCATTGAAATTCTAAGTCGTAAAACTAAGAATAATCCTGTCTTAATTGGTGAACCTGGTGTTGGTAAAACAGCAATTGTTGAGGGGCTTGCATACCGAATTGTTCATAAAGATGTTCCTTCAAACTTATTAGATAAAACAATTATTGAATTATCATTATCATCTTTAATTGCAGGCGCTAGTTATCAAGGCCAGTTTGAGGAACGAATTAATAGTATTTTAAAAGAAGTCAAAAAAGCAGATGGTAATATCATTTTGTTTATTGATGAAATACATCAAATTGTTGGTATGGGTAGAAATCAAGGTTCAAATATGGATGTTGCTAATATTTTAAAACCAATGATGGCAAGAGGAGAAATCAAATTAATTGGCGCCACAACGCTTGATGAGTATCGTTTATATATTGAAAAAGATCAGGCATTAGAACGTCGTTTTACAAAAGTTTTAGTTAACAAACCAACAATTCAAGAAACTTTAACAATAATGCGAGGTTTAAAGCCACGGTGAGAAGCCTTTCATGGGATTAAAATCCATGATAATGCATTAATTGCTGCTGTTAATTTATCTGAACGTTACATAAATGATCGTAATTTGCCAGATAAAGCTATTGATTTAATTGATGAAGCAGCTGCAAAGATTAAAACGCAAATTAATTCACAACCAATATATTTAGATGAAATTAAACGTGATTTACAACATTTACAAACTGAAAAAGCCGCTTTAGAACCAGAAAAAGATGAAAAATCAATTAAACGTTTAAATGAAATTTTAGAAAAAATTAAATTAAAACAAGATGAATTTAACAATTTAAATGATTTGTATTTAAAAGAAAAAAAGCAAATTGACGATTTAAAAAATTTACGGCAAAAAATTGAAAGAATTCAACATGATATTGAATTTTATCAATCTGAGGGTAAGTATGAAAAAGCATCAAGATTACTTTATTCAGATTTACCATTATTTGAAAAACAACGAGAAGAATTAGAAAATAAAATTAATGAGAATAATAATCAAAAAATGATTATTGATTCATTAACAGAGAATGAAATTGCTGATGTTATTGCGCGTGCTACAGGAATTCCATTAAGTCATTTATTAGCTGATGAAAAAACAAAATTATTATCGCTAAACAAACGAATTGCTAGTCATGTTATTGGTCAAGATGAAGCAGTTAAATTAATAAGTGATGCGGTAATCCGTGGTCGTGCAGGTATTAATAATCCTAACCAACCTATTGGTTCTTTCTTATTTTTAGGACCTACGGGCGTTGGTAAAACAGAATTAGCAAAAACTTTAGCAAAAGAATTGTTCAATTCAGAAAAAGCCTTAATTAGATTTGATATGAGTGAATATATGGAAAAACATAGTGTATCAAAATTAGTTGGTGCTCCACCTGGTTATATTGGTTATGAAAATGCTGGAGTTTTAACTGAATCTGTTAAACGCAAACCATATAGTATTTTGTTATTTGATGAAATTGAAAAAGCACACCCTGATATTTTAAATATTTTATTACAAATATTGGATGAAGGTAGCATTAAGGATGCTAAAAATAATGAAATTAATTTTAAAAATACAATTATTATTATGACATCTAATATTGGAGCAGAAGCTTTATTAGAAAATAATAAAACAAAGGCATTACTTGATTTACAAAAAACTTTTAAACCAGAAATTTTAAACCGAATTTCTGAAATTATTTTCTTTAATAAGTTATCAAAAGAAATCATTTTTAAGATTAGTGAAAATTTACTTAAAGAATTAGAAAATTTATTAGCTAAGCAAGATTATTTAATTAAATTTGATGATAATATTGCTCAAATCATGGTTGAAGATGCTTATAGTTCAAATTATGGTGCAAGACCTTTAAAACGATGAATTACAAAACATTTAGAAAATGAAATTGCTAAATTAATAATTGAAAATCGTATTACAAAAAACATAAATTATAAAATAAATTATGATCGTGAACTAGATCAAGTTTTAATTAAATAATAATAAAAATCACCAACAATAAAATGGTGGTTTTTTATTCCAAATATTTAATTAAAACCTTTTTTTAGTAATATAAAGTAAAAAAGATTATTTTTATTAATTAGTATTATTCTTATAATTATTATTAAATTTTTAGAAAGATAGACATTAGCAATGTTAAAAAATTATTTTCAATCAAGTTGATTATATACAACCTTTTTATCACGTTTGGTACTTTTTAAAAAAAGCACATATTTTTTAGTAGGAATTAGCTTATTTGTTAATTTAATTTTTATAATTATAAATGGTTTAAATATTGCTAATAATATAAAAACGTATTTGATTTTATTTTATCTTTTTACTAGTATTAATTCACTTTTAACAATAGTCTTTAGCACTATTAAAGCTATTAATTTATTCAAAGATTTAAAGGATGAAGGAATTGAAATTTTAGTCTTTTCAAAAAGTATTTCACGTAAAAACATTATTTTTACTAAAATAGGTTTTTTAATATTAAATATTGTTTTTTGAAGTTTAATAACTTATATTTTAAATATTATTTTTTACGTAGTAAATATAAAGAATAATAACGGTATTAATTTTTTTTATTTATATGCTTTTTTTAATTATTATTTCTGTGGATTAATTTTTGCTAGTATTGCTGCTTTAATTGTTAGTCGTTGATCAAATAAAGTTGCTATGATTATTCCAATTAGTTGTTTTCTCCCCTTTTTAATTGCTGGTGGTGTTGCTAATTTGTATTCAACATCTAAAATTAATCAAGTAGCCAAATACATGAATATTAATTATGATAAATATGATAGTAATACAATTTTAGATGTAGAAAAATTTTATTTAAATAATCATAATGATGAAGTTTATTTAATCACTAAAAATTTAAATAATCCACAATTTAGTAAACGTCAGAATTATTTTTTGAAAGCAGCGTTTGATCACGCAAAAAATGCATCGACATTTTTTCATGTCTTATCTTGATTATCTGTTCCTTATCAATTAAATAATAGTTTTTATAAGAATGATATTGATCCTTTTAGTGTTAATAGCCAACACGAAAATTATTTAGATAAATATTTTAATTATCATGGTTTAGAATCAAAATTATATGATTATAAACTTAACAAACACCCTCATTTACCACAATTTAATATTAATGAAAATCAAAAAGAATATTTTGTTCCTGGAGCTTTAAAAAATACGAGTCAATTCCCAACACTTGAGAATCGTAATTTAATTTATGCGAGAGAAAATGTAGATCGTTTTGACGTTAATTTCATTGAGGATGATAATTTATTTTCATCAACTAATGATTTTGTTGGTGAATTAAAATGAGCGGTTATTAGAAATACATTAGAATCAAAAGTTTTCAATAACTTTGCCAAAAAATTTTATGATAATTTTGATGAAGATATTGATAAACCACAAATTATTGCTGCTTATAATGATGTAATTAATGAACAATCATCAATAAATTTTTCAACAATCGTTGATGAAAATAGTATTCTATTTGCTAAAAAGATAAATAATTATTATGTTAAAAATCTTGTTGAAAAGAAAATTTATTTCATTGTAGCTTTAATGTATTATTTATATTTTAATGAGGAACGTTGAAATCTTTTAGAAAAATTATTAAAAAACGATAAAGTTATTAATTTTTATCGTCCATCATCAATAAGAATCAATGTAAATAATTATGCATATGATATTGGAGGGATTGGAAGTTATGAATTAATGAAAAAAGTAGTTAATAACAAAACTTTATATCGTTACCAACTACAAAAAAGTAATAATTATGTTTTTCAAACAGCAAAAGAAGTTTATTCTATTAAACGTGCTGATCAAATTGTTAATAAAAATTATTATTGATTAATTTGAATTTTATTTAGTACGATTTTAATTATAAGCTTGGCTTTAATTTATTTAAAACGAGATTATAAATAATTATAATTACTTACTAAAACTTAGTTTATTTAGTTCATATTATTAATTAAACCTACTACAGAAGCAACTATATAAGTTTCTGTGTATGAAATTGAAAGTTCTAACTCTTTTATTAAAATTTTATTAAGATAAACTTGGCGTTGATTCTCTGTTTTCAGAATTAAAATATCTCCATATCCATATTTTAATTTTAAAGCTTTCATAACTGCTTCTTTACAGGCAAAAACGCTTGCAAGATAGCGTTTTTTTTCCCTTGATGAATTAAGTTGAAAATAGTATTCTAATTCATTATCAATTAAAATCCGTTTTGCAAATAAAGGATTATTTAATTCTTCACGATTTCATTCAATAATATCAATTCCATGAACTAATTTCATTTTTTTATTTCCTTACCGGACGTTTCTCTCAAACATCAAAAGTGCTTTTTTTATCAACAACTTTTTCTAAAAGATCAATAAAATTATTTAGTGATGTTGTGTATTCACGTTCTTTTTCGAATTTTCTTTTATATTCATTAAGTTCTTCTATTTTTAATATTGCATTTTTATGTTCATTTATAAATATTTCTAATTCATTAATTAATTCGTCTAAAAAAACATCTACACTTTCAGGATCGTAACCAACTTTGTTAGCAACAAATTTTTTATTTTTATACTCATTAATTTTATTATCATTTACCATGCACACACCTATTGATTTAAATTATCTTCTCTACTAGTTTAAATTATAACTTAAGAATTTAAATAATAAATATTCATATAGGGAATTTTAGGACCAACTATTGTACTCATATCTACATTAATTGTTTCTATTTTAATCGAATCTATATCTTGTGAATCGTGGTTTTGTTGTGATGAACTAGCCATTACAATATTTGTTATGCCTCCAATCATAGAAATAGCTGTATTAGCAATAATTGTCCCAAATAACATTTTATTTCAAAAACCTAATGCTCCAGAAACAAGTGGCATAAATCCTCCATAAATCATGTTTTTATTAATTATTCTTTGCATATTAAAAAAATGGAGTTCCTAAACTAATCGTTGAATTCATTGGTTTTTGTGCGATTCTTGCATAAGTGCGAGCAACAGAGTTATCGATCATTTGTTGAGGATTAAATTTTGGTTGAGGGTTAAAAGCATAAACTAATTGTGAAATCATTCCTAAGAATCCAACAACTGTTTGACCTAACATTGATGAAATTAAAGCGTTATTTCACATATAATTAAAAGAGTTAGAAGTTGAAAAACCACCATAATAAACTTTTTTAAAATTATCAGATATTTTTTGCATATTGTTAATTCCTTTCTTACATTAAAAGAATTAGTTAAAAAAGAAGATTTTATTTATTTTTAATAATTTGTTGAAAGTTACTAAAGTGTTCTTTAGCGACTTGTTTTAAAATTTTTAAGCCAAATTTTTGAAAAATATTACTAGCGACGTCATTAACTAAAGGATTTGAACTTCCTAACGGGATTTTAACGTTAAATTGTTCTTCAATAATTGCAATCATTTTTAAAAACATAACACGTGCAATTATTGACGCCAAAGCTACACTTGTATAAATTGATTCGGCTTTAGGGATTAATAAGTTTGGTTCATAAGGTTGAAAATTGACTTTTTGTAAATATTGATTTCAGTTTTTTAATGAACTAAAAGCATCAACAACACTAAAAATTGAATATTTAGCGTGTTTGTGAATTTCACTTTGTAATGCTTTATAATGTAAAATAGTTTTAAGAATATGTGCATTATTGTATTCTTTAATTAATTCATTGTATTCTTTAGGATTATAAGAAACAATTGTATATGAAATCTGATTTTTAATTTGTTGATATATTTCTTGCATTTGCTGATCATTGAGTTTTTTAGAATCTATGATTTTTAAATGATTTATTTTTTTAAAATCACTTTTTTTTAAACTCACTGCGCAAACAACTATACCTCCAAATAAATCGCCAACTCCAACCTCATCAGACCCAATAATTACTTGGGCACTTTCATAAATTTCTGATTGGCTCATAACATTTTGATTATTTTTTTTAGTGTTTACTTGTTGTTTTATAACATTAAATAGTGTTAAATCTTCTCTTTTTAAAAATGTGTTTAAAAAAGAATTTATATCATGAGTATTAATAGTACATGTGTTAGTATTGTAAATAATGATTAAAGTACCATCGATCTTAAAAGTATATTTAGCATAATTATGGTTGTTATGTACTAAATATGGTCTTCATAGAATTAAGAATTTATCAACTTGATTAGATGTTAATTTAACAGAAACAATTGCCATAAAAAACCTTTTATTAATGTCTTATAATAAAATTATAAGTTTAAACGTATTTATATTGTTTATAATTAATTAAATTAGTTTAATACTAGGAGTTCGATTATGAAATTTAAGAAGTTTGATAGTAAAAAATCGATTTTACGTTTTTTACAAGTTGATGAAACAAAACGTGAAATTAATTTGTCTTTTAATGACACAACTGATTTTATTTTTTTTCTAAAAGAAATTGCTGAAGATCCTAATATTGCTAAAAAGAATGCTAAAAAATTAACTAGCTTATACATTGAAATTAGTAATAAACTGGGTAATGATGACAACACTGATTTAAGCGTTTATGAAACAAAATGTAATAATTTAATTGAAGCCTGTGTTATTGATGAAGCAATCTATTTAGTTAATATGGCAAAGAACATAGTCATTTTCTTAGCTCATAACAAACTTGATCTTTTACAAAAAGATTTTTTTGATAAATTATTATTAATAGAATCGCATTTTAATTTTGATCAAAATCTTACTTATTCATATATTAAAAACTCATCAATTGCGTTATTGATATTTCATTTAAAGCAATTTATTAACATTTATGATGAATTTTTAAACAATTTTGAAAATGTTATTAATCATAGTTTAGAAAATTCAATTTCAACTAAAAATAAATTAACCCAACAAATCAATCATTTTAATACTATAAAACGTCGTCCTTCATAATAAAACAAATAATTAAGATACAGTTGCATTATAAAAACCCTTGGATTCATAAATAATCTAAGGGTTTTTATAATTTGATATTCTTTTGTATGTTATGATCTTAAAACTTTATAAATGAAATATGGACCAATTAATAATGGTAATATAGCACCAACGTGATAAACACTCATTCCGATCAATGAAATACCAATTCAATAAGTAATTAATGTTGACAACATTCCATATATCCCTGAATTTAATATTCCGTATGTGAAACTATTATTACGACGACGCATTGCTGCATTACCCGCTAATAGACCAACATAAACAAAATCGCCTGATATTGAATAACTTGCACCTACCATTATACCAACAAAGATTAGAGCAATAAAAATTTGAAAATTAATTTTTATTCCTAATTGTTTTGCTACTTGTTGGTTAGTAGCAATAATGTTTAGTTTGTATGAGTTAAATAATAATCATAATAAGCCTAAAATAACAAAGCTTGCTGCAATAATTAATGAATTTAATGGTGCTTTTTGTACTGACCCTACAATATGGCCAACAACATAATCATTAGCGTGATAATTTAATCAACCACGAATACTTTGTGCAATTGCAATTAAAAAGAATGTTAAAATAACTCCTATTAAAATTAGTTTTTTGTAATTAAAGTTTTCTTTTTGACGTGAAACAAAGAAATATAAAACCATTAAACAACAACTCATAAACATAAAAATTCATGGTTCTTTTTTTTGTAAGATGCTTAATTCGTTTGCACTTGTAAAATCAACTGGGATACTTAAAGCACATAATAAAATTAAATTAATTGTCCCAATACCCATTATTGATACATCTGCAAAACGGTTTCGTGTGATGCGTTGTAAGACATAACTACTTACACCTAAAGCAACTCCAGTAATAATTAATTGTACAACCTCTTGTCATATTACTTTAGCTAATTCACTAAAGCCTAACTTATTAGATAAGGTAACAGAAAAAATTAAAATAATGACAACTAATAAAGCAATCAATAAAGTTATTGTTATTAACAAAGGTTTAAAATGATATGGAATAAAACTTTTTTTATTTAATTCTAAGATGTGATTTTGTCCTAATATATTTTGTTTATCTTTAAAAAAATTAAATTTCATTTTCATAGTTATTCCACATTCCGACGGTATTGTTTTGAAACAAAAATATATGCAAAGACTGGTAAGGCAGCGATAGCTGAGAATAAATTAATATCAAATTGATTATAGACATTAATTAATCAACGTGAAGATGTTAATAGTAATAATGAAATTACAAATGATAATAATAATTGTTTAAAACTATTGCCTTTTGGATTTAATATTCGTGCCACATGAGGCGCTATCATTCCTAATAGTGTTAAAACACCAATTAAAATGATGCTTGAAACAGTTGCGACAACTGCAAAAAAAGCTGTTAGCCAAAAAATCAAATTAACTTTAATTCCTAAAGATTTTGCTAATAAATAATCTTTAGCAATGATTGTATAATATGGCGCCATAAATAAATTTGCTAAACTAGAAATAATAATTAATGGACCACCTATACATAATTGTTGTCATTGAAAATTAATTGGAGGTTCAACGAAACCTGTAATTTTAATGTTTATATCTTTTACAAGGTCTTCTAATAATACATTAATACCAGTAATGATACCTCCAATTGCAAATCCTACTAAAATTGGTTTAAAACTTGCTTCTAAAGCATTTCCTTTTACCAAGAAAAAATTAATTACAATTAGACATGAAGCAAAAACAATACCAATAATGAAAGGAAGATATAAAATTTGATTAATAGCAAGTTTACTAATTGCTAAACCTAAAATTGCAGCAGGTAAATATCCTAATGTAGTTGGTGAAGCTAGTGGGTTTCGTGAAACTACTTGCATACTGTACCCACTAAATGATAATGTTAATGCAGATAAAAAGATTGCAATTGGAGTAGCAATAAATTTACGAATATAAGGGGCACTTCCCTCATTTATTTCTTCACCTACATTTTTAAAATTCATTAACTCTTTACCATCAAAAAGACAATAACAAATTAGCACACAAATACTTAAAATAATTAATAAAGATGTTATAAGTCTTCTAAAATGTGTTTTTCAATCTAATTTATTTTTAAATTTTAATGATTTATTCTTAAACATAAAAACTTAATTATTTTTGTTTATTTTCTTTTGCTGGATCTGTTCAACTTACAACTTTTGTTCCTTCAACAGATTGTAATGTGTTTGTTGTACGATCATAGAATTCAAAATTAATTGGTGAACGAGAATTATTTGAATCGCCACGTTCAGTAGGAACAGCTTTTGGTATTGCATTGCCAATAAGAGTTTTAAAAGAACGGTTAATATGAGAATTATTACCACCTAGAGCATATAAATTCATACCAGCAACTTTTTGAGCATCACCAGTGAAATCTTTAAATTCGGTTTTTGTTTCATCTACTAAATCAGCTAAAAATCCTGGTAGTTTAGTATATGTTTTTCCTTTTTCTTTAACGAATGAAGCATAGTCTGGTTGAATTCCCAATGCCACACAATGATCTCATAAATTATAGAATCAGATTGCAAATTTATTATGATCATTATTAGAATTTTTTGGACGAATGTGTTTTAATGTTTGTTTTGATGGCATAGTTCAATCAAGACTAACACTTGCTAATTCACTATCACTTACCCCTAATCTTTTAGCAAGTGATTGAACTAAACGTACTGTTCCAATAGGTCCTCAAATTGAAGAATATTCAAATTGAAAATCAACTGGAATAATTTTGTTATTATTAACAGTTTTCTTAAAGAATTTTTCAAAATTTGATTTTAAAATCTCTCCATCTTCATTGCCTTCAAGATTGTTTGAGTCATAATTAAAGAAAACATAATCCGCAGTTCCATAAAAAGCTTCTTGCATTGATAGCATTCAATCATAGTCATTTTTTGACGCATTATTTTGGGATGCACCAATTCCATGTCAGTTATCAAAATATTTATCAGCATTATGGTTTTTTGGTTTAGGAAAACGTAAACCAAACCCAGGAACATTTGAATCATCTGGTTGAGAATACAATAAAGGGAATAAATCTTCTTGTTGGAAATAGTATAAATCTAATTTAGATGCATCTGAACTATTAAAACTTTGTGTTGATTCTGCTCATAAAACAGTTGTATTATTATCTCTTAATTTTTTAATAGTTGGTAAATTAGCAACTTTTCTAACATATTCACGAATCTTATTATTAAGTTCTTTAGCATACTCTAAAAAACTTGTTTTACCGCTATTTTTCAAAGGTTCATGTGATGTAATTTCATAACCTGCATCATCCAATGATTTTGCAAACATTTCAAAACATTTATAAGGGTCTGTTAGTGAATTAAAAAAAGTACTACTATTATTAACATCACTTAAGATATATTTTTCACTAGATTTAGTTGCATTTTGTTTTGGAGCTTTTTTTGAAATTAACTTATTAAGTGTTGTTTTTCCTTCCTCTTTAACGTTATTGTCTTTTCTTGCACATGCTGTAGCAGCAACCGCAATACTTGAAATTAAAACAGTTGAAAAACCTACTAGCAATACTGCTAAAGATTTTTTATTTTTCTTAAACATATTTTTATTATCCTCATTTTTAAAATAATATTGTTTTGTTTTGACAAACAATTTCATTCTAATAGTAAAAAGAAAATATTCTATGATTTATATACCAAAAAATCCACTTTTTGATGTCCAATATTAGTTTTAGAAACAATTCCACCGCCAATGCAATGATCATTTTGGTAAAAGACAGCATATTGCCCAGGAGTAATAGCTCTAGCTGACTCATAATTTATTAGTACATTTTTGGTATTTTTAATAGGTAAAAAATCGTTTACTATTATTAAATCTTGTCGGTGACGAAAGCGTACCATAATTGGAATTTGAGGATTATATGGTTCAATAAAATTTATATTTTCAATTAATGCTTGAGTTGAAATTAAATATTGTTTTTCAAAACTTGAAGGGGCAACGTAGATTATTTTTTTATCGATATCTTTATCACAAACAAACATTCTCTCATTCATGCCACCTAGATTCAATCCTTTTCGTTGTCCAATTGTATAATACATTGTTCCTATGTGATGACCAATAATTTGATTATTGACAATATTTATAATTTGCCCTGGTTGGTTAGGAATATAATTTTGTAAAAAAGTTTTAAAATTACGTTCACCAATAAAACAAATCCCTGTTGAATCTTTCTTATTAGCTGTAATCAAACCGTGTTTTTTAGCAATAGCGCGCACCTGTAGTTTAGTTAAATGCCCAATAGGAAATAGCACATTCTGTAGTTGATTTTGGTTTAAATTACACAAAAAATAAGTTTGATCCTTATTTGTATCAATAGCTTTTAGTAAAAAAGATGAATTCCTATCATGCTTAACTCTTGCATAATGACCCATTGCAATATAGTCAGCTTGTAATTCATTTTTAGCATAATTTAAAAAAGAATCAAATTTAATAAATTGATTACATAAAATATCAGGATTAGGTGTACGGTTCTTTTGATATTCGCTTAAAAAATATTCAAAAACGTTATCTCAATATTCTTTAATAAATTCAACACGATATAATGGAATACCGATCTTTTGAGCAACAGCCTGAGCATCTTGGTAATCTAATTCACTCTCACAACCCTGATGCGTTTTATTAAATTCATGGTTATTTTCAAAATTTGCAATTGTATCCCAATTACTCATAAATAAACCAATCACTTCATAGCCTTCTTGTTTTAATAATAAAGCACTAACAGAAGAATCTACGCCACCAGACAAGCCAACAACGACACGTTTTTTTACTTTAACTTCCATAATATTTAGAAATCACCATCTTTAATTATTATAATATTATATAATTTTTTAATAGTGAATTCTTGATGCGTGAATTTATTTATAATATTAATTTAAGAGGTATATTAAATGAAAAAAGCAATTTTGATTTCCATTTCTTCATTAATTGGGATTAGTGCGATTGTCGCAACTAGTACTTTTTTGTCATTATCAGCCAAAACAAATATTTATGCAAAATTAATAAAATCAAGCGATCTTAATCAAAGTTTAAATTACACTAATTTATCAGATGCTAATATTGGAATTAAAGAAATGCTTTTAGGTACTAATAAAATTAATAATGGAAATTATGTTTTATATATTGGAACACAATCAAACCTTGATAATTTAAATTTTGTTTATGATAATCAAACTAACCATATTAATTCGATTAGTGATTTAAAATATAATGATAATTTGAAGTTTAATGGATCATTAGCAAAATCAATTAATAATGTCAAAAATTATGCAGATAAAGGTGTTTATGAGCATGTCCCAGAATTCTATAGTTTTATTGATTTAATGGATACTAATGTTTTTAAACAAAAACAAGATTATGAAAATTTAATTAAACAAAATAAATCAAGCACTATTAAAGAAGATCAAAATTGAGCTAACAATGCACCAACAGATTATTCTTTTGATGTTAATAAAAAGTATAAAGATAAAAGTGGAAGAGAAGTTTATTTTCGTAACGACACTCAAGCTGTTAAATTTCGTGAAATTTTAAATTTTTTAAAAAAATATTTATCAAAAGAAAAACTAATTGAATTAAGTACATCACAAACTCCTGGAATTGTTTTGTTTTATTCTCAAGACAATCTTAGCAAGGGTCCACAAGTTTATGCTAGTTCAAAAATATATGACAAATCAAAAAATGAACGTAAAGAATCATATGTTTCTGGGGACAACGTCTATAATTCATCACGTTTTGAATTTGGTGGAGATTTAAACGCTGCTATTTATAGTGTCTATGGTAAAAAATAATCAAATATAAAAACTATTGGTCCTTTTTCATACTTAATTACAAGTGTTCATATTTAAAAATATTTTTAATAATTAAAAATAAATTCAAAAAAAATAATATAAAAATAAGTTTAATGTTATCAAAAAACATTAAACTTATTTTTTAGTTGACTTTCTATATATATATATATATAATATAAAATAATTGGATAAAGAAATGTCGTGATTTAAGAGGAATTGATTATGCAATACTAGTTTTTTTGGTTTAAGATAGATTTATTAATAAAAACAAAACGAGCACGCTCAAAATTATTAGAAGTATGATTTTTTAAAGACATTAACAGTTCATCATTATCTTTAAAAATAACTTTATAATTAATTACTTGTGGATGATATTTTGAAATTTTTATAATAATATCATTTCATCATTTGTCTTCTAAATTATTAATAACTCAAAAACCTAAAATTGTTAAACGCTTTTCTAAATCTTTAGTAATTACGATTGTTAATCATTTATTTGATGATAAATTAAAACCATCGATAAAAATTTGATCAACATCTATATTATTTAGTTCTTGTTTTTTATTCTCTAAATAATCAAAAATTTTTAAAGCAATTTCATTACATAGTTTATCAACCATTTTGTCATTATAAATATTATAAATATCTTCTAATATTTTTTCATATTCATTTAATTGTAAATTATTGATTCCCAACGATAATAAGCGATATTCAAAATGTGAAATATCAACTTGGTATTTTGAAATAATTTTTGATTCAAAAGCTCCATTACGATCACGTGGAATATTAATTGAAACTTTTAAATCGTTCATATACAGTTGTTTTTTAGAAAAACCATTACGATAGTTTTTAGAATTACTACTTAAATAACGTTCAAAACGTTCATAATTTAAATGATATTTCATTTCGATTGTCAATATATATTCTAAAAAGGTTCTTGTAAAAGTACTAATATAGTCATCTAGAATTGGTTTGTGCAAATACTTGTATTTTTTAACTAACTTCTCTAGAATTGTAAAAATATTCTTTTTGTCCATAAAAAAACTCCATTGCAAAATAACAATGTTTAAAATAGATTCATTGTTAATTAATAATATTATTTATTAACATCACTAAGATTATCTTTTGACTTAGTGTGAAATAAAATTGTTTTTCTTTCTTGTTTTGTAGCGATGCTTAAAATATTTGTTTTGTGTCGATATATTAATAAACTTGCACTAACTAATAAGAAAAACCAAACATATCAGATTCCTTTTATAACATAATTTAAATCAAAAAAATACGTAGTGAAATTTTGATTATAGTATGGTATGTAATTAATTCCTGGAACTAATACTAAAAAACAAGTGAAAGTTGCAGTAATCATAGCAGATAAAGACATATATTTAGTAATTAGTAAAATGACCAATAGTGTAATGATTCCAATTAGCGCTACTATTGGATCAACTGTAATTAAAAAACCGCCATAACATGCAACTCCCTTCCCGCCTTGAAATTTAAAGAAGATTGGGTAACAATGACCAATAATGGTGAAAATACCACCAAAATAAACTAAAATGGCTTGATTAAAACCATTACTTAAATCAAATGGAGCGTCTAAAGCATATTTGTAAATAATGTAAGTTAAACTAACTGTGGTGATTGGTTTAATAATATCTAAAAAAAGCGTTAATATACCTAATTTTTTTCCGTGAACACGTATCATATTAGTCATGCCTGCATTTTTAGAAGCAAAAAGACGAACATCTTGTTTTTTAAAAACTAAGGATAAAATGATGCTTGCATTTAAACTACCAATTAAATACGAATATAAAGGAGATATGATTAATGTAAGAATATAAGCCATGACAATGTTATATACTTGATCCATAAAAACTCCTTTCTTTTGACTTAATTAATATCATAAGTTTTAAAACAAATAATTTTAGAAAACAAACATTTTCATATTTGTCAATATTAAATATAACACACAAGTTCAATCTTAAATATTAAAAATAAAAATTATTTTGAGCATAAATTTCAATTATGCAATGTTTTTTGTCTATAATAATATTTAAATATTAAAGCGATAAGGATGTGATAACTTGATTAATAATAAGAAATTTATTAATAAAACATTTGATAATACTAACCAAATACGATTGCAAAATAATTTAAAAACTTATGCTCCTAAATTTTTAAATTTAATTATTATTAATAATATTTTTGTATTAATTATGATTATTAGTTTGTGTATTTTCTTTTCTGATCCACATTTTTTATATCGAAACGATGTCATTTCGAACCAATATCGCCAATTTATATTTACATATCAGCAAACAGCAATCGCAATTGTTTTATATGTTTTAGCATGATATATAATGGTGAATTTTTTCTTCTTTGGCTATTTATTAGAATATGTAAAATTAAATAAATTTGAAACATTTAATTTTGCATTAGCGTCAATTTTATTTAATCCTTATGCTTATGTTATTATTGTTAAAAATTGAACATCATGATCTTTTTACTGACAACGTAGTTTTTATCATGTATTAAGTGAAAATAATAAAATTGAATTTAATATCAAAAATTCAAAAACAATTTTCGCTTTAGTTTTTTTAATTATTGCTATTCCTTTTATAATTTTTTCATCGATTGATTATGTCCCGAAGAATCCAGGGCGTTTGATTATTTTTAACAAAAATAGTTTAGGCGAAAAGGTTATTTACACAAACAATGTCTGATTTCATAATATGCATTATTTTACTTCTCAAGGGAATTGAATGTGTATTGGAATTGCATTCTTATATTTTATTAATCCAAAAGCACGTTGTACAAAAAACAATCGTGTTTTATTAATTGTTTTGTCTTACATTTTAATTATTAGTTCAATTTGATTATGTGTTTTGTTTCCAATGTTTTCACGGCAATCAACTTGAGTTTGATTTAATAATATGGTGGGTTTTTATAATCATTTAATTACTCCAATATCTTTTACAATTTTTGCTTATTATTGTATTATCAAAAATAAATATGCAATTCACATAAAGTATTTTTATGCATGAAAAGGATTTATGTTTTATGTAGTAATTTATGCAATGTATGCGCTATTTTTACCTCTTTTAGCAAATGTTACTGTTTATGGAGCAATTACGAATATTTGACCAAGCGCAAATGGTAATCCTATTTTTGTAACAATGTTATTCGCACTAATCGGTTATCAAATTCTTGTTTTTAGCATAAATTGAACCATATTAAAATTCATTAACAAATACAAAAATAATAAAATCTTAATTGGCTAATTTTTTTAAATGCAATTTTATTACCATGCATTTATAGAGCATAAATAAAAAAAGTGAAAGCAATTTATAAAATGCTATTCACTTTTTTTATTTTATTTAGCAACTTTTACAACTGCAAATTTAATAACTTTATCATTATATTTAAAACCATCACTAATAACTTCAACTACATGCATTGATGGTTTGTTTGTGTTTTCAACAACTTCAAAAACTTCCATTATTTCGTCATTTAAACTATCATTAACCTGTGGAGATATTTTTGTAATTTTTAGATTATCTAAACCATCAATCATGTTTTGAGAAAACATTTTAAACCCCTCAATGAAAGCTTGGATTTTAGGATCATCATAGTTTTGATTTACAATTTTCGTAAAATGATTAATTGCATCTAATAAAGGTTCCATTTTAAATTTGAAAATTTTAAAGACAGCATCATTTACTTTGGCTTCATGGCGTGCTTCTAGTTCATTAACTTTTTGTTCTAGAATTTGTTGTGCTTGTTTTGCTTTTGTTTCTAATTGTTTGATAAAATCTTGATTTAATTGACTAATTTGCTCTTCTAACTGTTGATTTTTATCTTTTAAAAGTGTATTGATATTTTGTAATTCTTTATTTTTCTTTAATTCATGTTCATATAATTCTTTATATTTAAATTCTTGCTTATTATGATTTTTAGTTTCTTTTTTATCAACTTGGTTGTTAACTTTGTCATTATTTTGGTGTTTGATGTTTTCATTATTTTTGCTCATTATTTACTAATCTCCTTTAATACTTTATTTTTATAATACTCAACATTTTGGTTTGTACGAGTAGAAATATTTTTTAAATAAGCAATTTCATCTTTGGATAATTCAACTGGCTTTGCATAACGAATTGTAGTATAAAAATCTCCATTCTTTTTACCAAAAATATTTGATTTTTTATTAACGATTCCTGATCCACTAATTCTAAATTTTTTACCTTCAGGAGTATTTGAAGGAATTTCAATGGTTTTAATACCATTAATTGTTACTACTTCAATTATTCCTCCAACAATTGCTTTGATGGGATCAATGTAAGTTTCTGAATAAATATCTAAACCATCTAATGCAAAATACTTTGATGGTTTAACACTAATGTGTAAATAAAGATCAAATTCTTGATTATTGACAATATTACCTTTTTTGCTTACTAATAATTTTTCACCTTCTCGTGTACCTGCTGGGATTTCAACAGTTAAATTAACACGTTCATTGATAACTTTTTTACCATAACATTGTTCACATTTGCTACTAGCAACTTTACCTGTTCCTTCACACTCATGACATTCAACTTCTTGTTGAATTGTTCCAAATAAGGAATGAACATTTTCAATGCTAGTTCCTCTACCGTGACATTTAGTGCAAATTTTGACATCTTTAGGATCTTTTGCACCTGTTCCTTGACATTTATGACACGTAATTTTACGATCATAATTAATATTTTTAGAAATACCATTAACAGCTTCCATAAAATCTAAATGTAAACGTAAGTGAATATCATATGTTTCATATTGGTTTGATGAATTATGTGAACTAGAAGCATTATTAAAAAAACTGCCAAAAATGTCATTAATATCAAAATCAACACCACCAAATCCTCCACTAAACCCAGAAAACCCACTAGCACTGCTAAAACCTTGTGGACCATCATGGCCAAATTGGTCGTATTGTGCACGTTTTTTAGAATCAGATAAAACCTCATATGCTTCATTAATTTCTTTAAAAAGAGTATCATCAGCAGATTTATTACGATCAGGATGGTGTTCTTTTGCTAATTTACGAAATGCCGCTTTAATCTCTTCAGGAGTTGCTGATTTACTAACACCTAAAATTTCATAATAATCACGTTTTGCCATACTTAAAACATTCCCCCTAAATATGATAATTACAATGATATATTATAACCTAAAATAATAAAAATTAGCAATCATCATATCTGATTGCTAAAAATAATCATAATTAATTATGAAAAAATAGACAACGTTATTCCTTTTTTTCATGATGTTCATGATGACAAGGTTTGCATAAATCCATTTTATTCATATATAAATTAATTGTTTTGTAATTTAATTTAACATTATATTGTGTTTCAAGAATTTTTTGAATTTTACGACAACCAATATAATGCTTATGACTTTTACAAATTTTTTCAATTAAATCCATATTTCTTTTTTTAATTGGACAAACTTGATCTATTTTTAAGTCTTCTAGTCATTTATAATAACCAGAAATTGATACATCAAACAACGGAATTAATTTCGTTAAATGCATTTGGTGGTTTTTAATCAATTCATGAATCATTTTAAATTTTTCTTTTTTTGATTCATGATGACGATAAGCATTAATTACCTTAATAATATGATTTTGTTCTCATTCTTCATAATGATTAATTCTTAATGCATTGATATCAATGTTATCGACAATTTTTGACATAAGCAAAATAATTCCTCTCTTGACTATACTTTATATTATATTTGTTTATGTAAATTTTTTGCAAGTGATAACGTATTAAATAACCTACAATAAAAATTGCATACATAAATAAACACATAATTAAAGCGTTTAATCGGTAATTTAATTGTAAGAATTGTGCTACGGTACCATATTGTGCATAAATTGGAGAATATCAATCACCAAAACTAAAACCACTAGCGTTATTCTTAATATTAAAAATTAATATCATCACGTTAACATAAATGATATAAGTAATTAGAATAATATGAATGATGATATATATCCAGTATATTTTAATGGTTTTACGCGTGTTTACAAATAAATAAAATCCAAAAAGACACAAATAAAAGTGTAAACTAAAATAAATGGGATTAGGCTTGTCCCCTATAAAAATATAAGTTATTAAACGATTTCATCTAATTGGTTCATCACCAATTAGTCCACCAATAATTGTTAACAAACCAAAACAAAAGCACAAAAAACCTAAATGATTAATAATAATCTGTTTTTTATCAAAAATGGCTACACATGAAATTAATACTGAAAAACAAGGACATCAATCTAATAAAAATGTGCGTGAAATTGTAATAGATGCTTCATATAAATCAATATCATCACTAAATTTATCCCTTAAAACATTGATTATTTCAATATGATTAATAAAAAAAACATGAATATTTGGTGCTCATCGCAAACAACAAAAATAAATTAAAAAACTTATGCTAATTAGAGTATTAATAATTCATTTAGTGTTTATAAAATAATTTCATGTTAACCAATTATGTTTTTTAATTTGTATTGGTTTAATAAGAAAATTAGCTAAACCAATACAAGTAATGCAAAAAATAGTTAAACCAATTAAAAAATAATTTGCTGACAAATAACAAGCACTCCTTATTTTTTAGTATAATTCTAAGTATTAATTAAACCAAATTAATGTTTAACTTTAATAATTATATTACAACATTATTACGAGTCTTAGTAATGTTGTTTTAAGTAAATAATTCAAACAAAAAAAGGAAAAAGAGAGTATGAAAGATTATAAAAGCACCCTTAATATGCCTACTACAGGTTTTGAAATGCGTGCTAATTTAAACGTTAAAGAACCAAAAATTCAACAATTTTGAGTTGAACACCAAATTTATGAAAAATTGCTAACTAAAAATAAAGACAAGAAGCCTTTTATCTTGCATGATGGGCCGCCATATGCAAATGGAAATATTCATATTGGTCACGCTTTAAATAAAATACTAAAAGATTTTGTTGTTTCATATCACAATATGAATAATTATTACTCTCCTTATATTCCAGGATGAGATACACATGGTTTACCAATTGAGGTTGCCTTATCTAAAAAAATTAAATTATCTAATCTAAGTGTTAATGAACGTCGTGAACAATGTAAAAAATACGCTTTAGAACAAGTTAATAATCAAATACAACAATTTTTAAGATTAGGAATGATATCAGATTTTAAACAACGCTATTTGACATTAGACCATAATTACGAAATAGATCAATTAAAACTTTTTACTTATATGCTAAAAAAAGGTTTTATTTATCAAGACTTTAAGCCTGTTTTTTGGTCATGATCATCACAAACAGCATTAGCAGAATCAGAGATCGAGTATGCTGATCGTCAATCTTCAGCTATTTACGTAAAAATGAAGGTAGTTGATCATAATGATTTGTTTACTGATAAACCAACATCATTAGTGATTTGAACAACAACTCCATGAACATTACCAGCTAATTTAGCAATTGCAATTCATCCAGAATTAGTTTACAGTTTAATTGAATATAAAAATGAAAATTATATAATCGCTAAACCTCTTGTTGAAACTTTTGTAAAAAAAGTTGGGTTTGAAGATTATAAATGAATTAAAGATTTTAAAGCTAATACTTTAGAAAAAATAAAATATATTTCACCAATTAGTAAAAAGCATGCTTTTGTGATCATGGATGAGTATGTTAGTGCTAATGACGGAACGGGTTTAGTTCATAATGCGCCTGCTTTTGGTTTAGAAGACTATTATGCATGTAAAAAATATGGTATTGAAACAGTAGTTATGATAGACCAATTTGGTAAATACAATGCATTAGTAAATGATTTAGAATTAGAAAATATGTTTTATGAAGATGCTAATCAAGTTATTTTAAATCGTTTGATGAATGAACATTTATTAATCCATCATGAAGTTATCACTCATAGTGTTGCACATGATTGAAGAACAAAAAAACCTGTTATGTATCGTGCCACAAAACAATGGTTTGTTTCTATTGAAAAAATTTTACCTAACATTTTGCAGACCCTAAATAACGATGTTAAATCAACTAGTTTTAGAGGAATTGAAAGAATGCATGAAATGATTGTTAATCGTAAGGAATGATGTATATCACGTCAACGCGTTTGAGGTGTTCCTATTCCTATGATTTTTGATGAGAATCATAATGCAATTATGGATCATGAATTAGTAGAAAATATCATTAATGTTTTAAATGAAAAAGGTGTTAATGCATGATTTGATTTAGATGTAAATGCTTTTTTAACACCTAAGTATTTAAGTATGAAAAATAAAACTTTTTATAAAGAAAAAGACATTATGGATGTTTGATTTGATTCTGGTAGTTCATATAATATTTTAGGACACTATAACCTAAATTATCCAGCAGACGTTTATTTAGAAGGTTATGATCAATATCGTGGTTGATTTAATTCTTCATTAATTACAGGGACAATTTTAAACAATCGCGCACCTTATAAATATTTAGTAGCACATGGAATGGTTCTAGATGGTGAAGGATATAAGATGTCAAAATCAAAAGGAAATGTTGTTGATCCATTAGATGTATGTAAGATTTATGGAGCAGATGTTTTAAGATTATGAATTGCTAATTCAGATTATCAGAATGATACAAGAATTTCAGAAGAGATATTAAAACAAAATGCTGAAATTTACCGAAGAATTAGAAATACTTTATTTAAATATTCATTATCAATCTTAAATGATTTTGAACCAAGTGTTGATTTTAGTTTTAATGTTCGTCAAGAAGAGCAATTTGTTTTAAATGAATTTAATGAGTTACATATAAAAGTTATTAAAGCATATGAGAATTTTGATTATCAAACCGTTGTTAAACTATTTAACAAATTTATCTTAGATTTATCATCATGATATTTTGAAAATATTAAAGATGATATGTACTGTTTGGCAATTAATGATCCAATTCGCAAACAAATTCAATCAGCAGTTTATTGAATTTTAAAAAATTCATTAATTGATTTAACACCAATTATTCCACACACAACTGAAGAAGCTTACTCGTTTTTAAAAGATGCTAATAAAAAAGAATCAATCCGTTTAGAGGATTTTTATGATCAATCCCAATTTCAATTCAAAAAAGGCATTGCTCATGTTAAAGCATTCTTTAGTATTAAAGATCAAATTTTTAATGAACTTGAAAATGCACGAAAAAATAATATTTTAAAGAAAAATAATGAAGCTTTTGTAACAATTGCAAAAAATTTGATTTTAGATGATTATTTGATAAATAATCCAAAACTATTAGCAAAATGGTTTGGTGTTGCTAAGATTGAATTTGCTAATAACACAAATGTGGCTAATGCTAATTTTAAAAAGTGCTTACGTTGTTGAAATCATTTCCCTGATGAAGAAATGTACAATGATGAATTATCAATGAATTGTTATAAAGTAATTAATAAAATAAAATAGAACATGAATGATAATTATTTAATTTTAAGCATTGAATCAAGTTGTGATGAAACAAGTTTAGCGTTATTTGAAAATAATAAATTAATTGCTCATAAAATTAGTAGTTCAGCATCTATACAATCACTTCATGGCGGTGTTGTTCCTGAATTAGCAAGTCGTTATCATGAACAAAATATTAATCACCTATTTAATGAAATTTTAAATGAAACAAAAATCAACCCTTTAACTATCACTCACGTTGCTTACACTGCAATGCCTGGTTTGCCAGGTTGTTTACATGTAGGAAAAGTTTTTGCGAAACAATTAGCTGTTTTAATTAATGCTGAATTAGTGCCAATCAATCATTTACATGCGCATGTTTTTAGTGCAAGTATTAATCAAAATTTGACTTTTCCATTTTTGGGATTAGTGGTTTCAGGAGGGGAAAGTTGTATATATTTAGTAAATGATTATGATGAAATCAAAGTTTTAAATCAAACACACGATGATGCAATTGGTGAATGTTATGATAAAATTGCTCGTGTTCTTGGTTGAAAATATCCAGGGGGTCCAATTATTGATAAAAATTATCAAGAAAATTTAGCAACATTAGAATTTATTAAATCACAGCCCGCTGCTAAAGATTTTAGTTTTAGTGGGTTAAAAACTGCTGTAATTAATTACATTCATAATGCTAAACAAAAAAAGATAAGTTTTGACCCTGTTGTTGTTGCTTCAAGTTTTCAAAAATTTGCAATTAATGAAATTATAAAGAAAATTAAATATTACTTAAATTTATACAAATTAAATCATTTAGCAATTGGGGGAGGCGTATCAGCTAATAGTTTACTCCGTAAAAAAATTCAATCTTTAGATGTTATATCATATATTCCAGAAATGATTTATACAGGTGATAATGCTGCTATGATTGGGGCTTATGCTTATGCTTTAATTAAAAATCATAAGAAATCAATATTAATAAAATAATGGTAGAAAGAAAAGAATTTAGTATGAAATATAAAAAAATTATAACGAGCGAATCTGTTGGTGCAGGCCATCCAGATAAAATATGTGATCAGATTAGTGATGCAATTTTAGATGAATGCTTAGCACAAGATAAAAATTCACGTGTAGCTTGTGAAGTTTTAGCTTGTAATCGTTTAATTGTTATTGCTGGGGAAATTACCACGCATGCATATGTTGATGTAGTAAAAACCGCTTGAGAAATTATTAAACCTTTAGGCTATAATGAAAATGATTTTACGATTATTTCTAATGTTAATAAACAATCAGTAGATATAGCGCAAAGTGTTAATAAAGCAAATAAAGATTTAATTGGAGCTGGTGACCAAGGAATTGTTTTTGGCTATGCTTGTGATGAAACACCTCAATATATGCCATTAACAACAGTGTTAGCTCATGAATTACTAAAAGAAATCGAAAAACAAAGACGTAATAAAGAATTTGTTAAAATTCAAGCTGATATGAAATCACAAGTAAGTATTGATTATTCTAATTGTACGCCTGTTATTGAGACAATGTTGGTAAGTATTCAACATGATGAAGATTATAACATTGAATATTTTAATAAAAAAGTTAGCACAATTATGGATCAAATTGCTATAAAATATGGTTTAAATACCGATTTTAAAAAAATTATTAATTCCTCAGGGAGATTTGTAATTGGTGGTCCAATTGGTGACACTGGTTTAACTGGAAGAAAAATTATCGTAGATACTTATGGAGGAATAGGTCATCATGGTGGTGGTGCTTTTAGTGGCAAAGACCCTACTAAAGTTGATCGTAGTGCTTCATACTTTGCACGTTGAATAGCTAAAAATGTTGTTGCAGCTAAGCTTGCTAAACAGTGTGAAATTCAATTAGCTTTTGCAATTGGAGAACCACAACCTGTGGCAATGTTTGTTAATACATTCAAGACTAATTTGGTTGATGAAAAGAAAATTTTTGAAGCAATTAATAAAAGTTTTAATTTTGATATCAAAACGTTTATTAATGATTTAGAATTGTGAACAACTAAATATTTACCAGTAGCAACTTATGGTCATTTCGGTCGTGATGATTTAAACTTAAGTTGAGAAAAATTAAATAAGGTTCAAGATTTAATAAAATATAGTAAATAAATTTAAAAAACGATACGCACAATAAAGGGCATATCGTTTTTTTAATAAATAATATAATGGTGCTCAAGAAGGGACTCGAACCCTTATGATTTCTCGGTTGATTTTGAGTCAACTGCGTCTGCCATTTCGCCACTTGAGCAAATTAAACATTAATTATTATAACAATTTTGAATTTAAAATACTAGTAAAAGATTAATTAATTTTAGTTTTGACAATTTGGACAATAATAAGTTCCCCGACCGTTGACCTTTGTTTTTTGAATAATTGTTCCACAAATTTGGCATGGTTCTTTAGCGTGTGTGTGAACTAATAACATCTTTTGAAACAAACCTGTTTCATCATTACCAAATTTATAAGTGTGGATTGTTGTTCCTTTATTTTTAATTGATAATAATAAGATTCGTTGTGCTTCTTTTGTGATCTTTTCATAATTTTTTAAAGTAAGATTTTTAGCTAATATTGTAGGAAAAATTTTAGCTGCAAATAAAATTTCATCAGCATAAATGTTACCAATTCCTGAAACAACAGATTGATCTAACAAGGCTGTTTTAATGGCTTTATTACTTTTTTTTAATTTTTCATACAAATACTGCGCTGAAAAATTATTATCTAGCGGATCTAAAGCAATCTTTTTTAATTCTTTTGAATCTAAAAAAGATTGTTGCTCATAAATGTGAAAAGTTCCAAATTGACGCGTATCATTATATCTTAATTGCATACCATTATTAAATTCAATAATAATATGAGTATGTGCTAAATTAAATCATTCCTCTTTTGCTTGATAATAAAATTTTCCCTCCATCCGCAAATGTACACTTAATACTAAATTATTACTTAAAAAAAATAATAAATATTTACCAAGACGTTTAATTTTGACTATTTTATGATTAATTAACAGTTTTTCAAATTCTTGTGGTGTTTTATTTTTTAAAATTTTAGGTAAATGAACAATAGTTTTTTTAATAAAAATATCGAGAACATGATGATTTAAATAATCAACAATCGTTTGTACTTCTGGTAGCTCTGGCATTTTTTAAACCCCCTTATATAGCAGATTATAATATGGATAAATTATTTATAATAATTTTGCATATTATTTATTTTTAAAGATGTGAATAACTCATCTAATTCTTGAGTATTATATGGTTTTAAAACAAAATTTTCTAATTTTTCATCAAACAATAAATCCGTTTGAATCGCCGCTAATTTTTTTGATAAAAAAGCCATATCTTTGTGTTCAATTAATTTATTTTTAACACTAGGTGCTATTTTTTCTAAATTAGTATAAATATTTTCTATATTATCATATTTTAAAAGTAAATTAATAGCTGTTTTAGGGCCGATTCCCTTCACGCCAATTAAATTATCACTACTATCACCCACTAAACCCTTATATTCAATAATTTGATACGGTAAAATGCCATTATTTAATAATGAAAAATTTGTTAAATTAATTTCTTGCATAACACTTGTCCCTGTTTTTAAGAGTTTAAGTTTTGTGTTTTTGTTAATTAATTGCAACATATCACGATCTGAACTATAAATAGTTGTATCAACATCAAACTTATTCATTAAATTACTAAAAGAACCAACAAAATCGTCAGCTTCAATTCCAGGTTGTGAAATTATTCGAAAACCCATAGCAACGAATAGTTTTTTTAAATAGGGTAATTGAACAATTAGTTTTGCATCCATAGGTTTACGTGTCGCTTTGTAAGCAGCATATTCTTGAGCACGAAAAGTTTGTTTAGAAGCGTCCATAGCAATTAAACCATAAGTAAATTGTTCTTGATTTAAAATCTTAAAAATCATTGAGGCTACTAATTTAATGGCATTAGTAGGCATTAATTGTTTTTCAACTGCTCATTCAGCTTGTTTATAAGTAGCGTGAAAGGCTCGATAAATCAATGAATTTCCATCAATAACAATTGCTTTTTTCATAATCTAAATCCTTTGCTTAATATTTACAATAAAATTAAAATGATTTAATAATTTAACTCCAAAAACATAAATACCTCCAATACTAATTTCATCAATTAAATATTTACTATTTTGAAAAATTGTTAGTGTTTGTTTTTTATTATCAATTAGTGTTTCTAAATAAAAGATATCATTCCCGTTTTTAGTTTTTGATTGCTTAATGTTTAAAACTTGTGCAAGAATGTTTGTTTCATAATTTGTGTTTATTTCATAAAAGTCTTTAATTGTATATTTTAGTTTATTATAATCAATTTCAGTTTTATAATTTGCGTAATTTAAACTATTCAAATCAATTCCTAAATACTGAATTTCATCATCTAAATAACGATCATTAACACTCATATCTTTTGCATAATCTAAACCCACTAAATTTAAATCAAAAAAATGACCATTCAATCCTGTTTTTTCAAAAATTTCATTCAAATTATTTAATAAAAATAAACGATTAATTCCAAAACTATCAAAAGTGCCGACTCGTATCAAAATTTCAATGTTTTTTAAACTAACATTGCCTTTTTTTAAAGCATCAATGTATGAAATATAATCGCTTAAAGTTGATTTTTGCAGAGCAGATTTGATTTTTTTAAGTAATTCATCACCAAAACCCTTAATTGTTTTGAAACCAAAACGAATTATTTGTTTTTGAGTATCAATAACAAAATCATTACTAAAATATTGAATATCAGGGCCCTTGATACTAATATTAAATTCTTTTGCTTCATTTAAGTATGATAATAATTTATCTTTTGATGCACTAGTATGTGATAATAATACACTTAAAAATTCTAATGTATAATAATGTTTTAAATACGCTAATCAATAACTAATATATGAATATGCTAGTGAATGTGAATGGTTAAAACCATAATGTGCAAACGAAAAAATATATTCAAAAATTTGATTAACCAATGGTTGTTTATAATTATTATTCAAAGCACCATTGATAAAATCATCTTTTAATTGGATTAAAATTTTTTCATCTTTTTTTGATATTGCACGGCGAAAATTATCGGAAGTAGCAATATCAAAATTTGCAATAGTTTTAACTAATTCAATCACTTGCTCTTGATAAACAATAATTCCATAAGTTGGTTCTAAAATTTTCCTTGTGGCTTCGTTTCAATAACTAAACTCTTCACGTTTAAAACGACGTTCAACAAATGTTTTAATATTTTGTTGTGGACCAGGACGAAAAAGAGCTGAAACAATAGAAATATCTTCAATATTCTGAGGTTTGACCTTTAATAGTACTTTTTTCATTCCGGGCGATTCTAGTTGAAATATTCCGTTTGTCCTTGCTTTAGCTAAATCTTCAAAGACAAATTTATCATTATAGTTAATGTTAAAGAGATCAATTTTTTTATTTTGATTTTCATAAATCATTTTTAAAACATTATCAATAATTGTTAAATTTTTCAAACCTAATAAATCCATTTTAATTAAACCAAAACGCTCTAAATATTCCATTGAATATTGTGATAAAGGACGGTCATTAATACCTAGTTGAATTGGAATAATATTTGAAATTAAACTATCACTTAAAATTATCCCTGCAGCGTGTGTGCCTATTTGGCGAGGAGCATTAATTAATTTTTTAGAAATTTCAAACAACTCTTTATGCAAAAGATACATTTCTTTTAAAATCGTATTTTTTTTAATTGCTAAATCAAGATCTTCATCATATTCAGGTTTAATATTTTTACAAATTTTATCAATTACTTTTAAATCAATTCCCAAAATCCGCCCTACATCTCGAATAGCCATCTTCGCTTTTAAACGTTGAAACGTTAAAATGTAAGCAACATGATCATTGCCATATTTTTCAAATAAATATTCAATAACTAGATCGCGTTTTTCATCCATAATATCAGTATCAATATCTGGCATACTTTTTCTGGTAGGATTTAAAAAACGTTCAAAAATTAAATTATGTTTAATAGGATCAATATCTGTAATATGTAAACAATAAGCTACTAACGAACCAGCAGCACTCCCACGTCCTGGCCCTACAATGATACCATTACTTTTAGCAAAATTAATAAAATCATAAACAATTAAAAAATAATCATCAAATTGTTTTTCATTAATAACATCTAATTCATATTTTAATCTTTGAACATAAATTTTATTAACTTGGCCATCGTTAGCATTTAAGCGTTTTTTTAATCCGCTAATACATAATTGTTTTAAAATTTCACTTGAAATAATTGAATTTTGTTTATCATAAACCGGAATATTAATTGGTAAATCATGAATTTGTACTTTTAATTCATTTAAAACTTTATTTAAATTATTTAAAGCAATTGGTGAAAAAAATGATTGTGCTAGATTATCATTTAGAAAATGATTATTATCAAAATCGCAACAATTTTTAAAATCTTCTAACTTTAAATCATTTTTGATTGCTAACATTGCTAAAAAAACAATTTTATCATTTTTGTTAGCATAGAAAACACTTTGTAATGCAATCGCATTAGGAGCATTTTGATCATGCGTTTGATAAAAATTAGGACTCTTAAAAACTATAGTTCCTTTTTTACAAACAATAATTAAGTCATCAAAATCATCTTGAATTTCAAATTCTTTACTTGTCATAATTCAAGATGATCATTTGATTAATTTTAAATAGCCATTATAATTTTTAGCATAAGCTACTAAAGTAGCATTTTGATATTCAAATTCTAATCCGATAATAGGAGTTAAATTATGTGCTTTTGCTTTATCATAAAATTCGATACAGCCATACATATTATTTAAATCAGTTAAGCAAACGTATGGTTGTTTGTTATCTAATGCGTATTTAATTAAATCATCAATACTTAAAGTACTATTAAGTAAAGAATAATGTGAATGCACATTTAAATTAATAAACATTATAAATTTTGTTGTTTCTCTTTAATAATTTTTTTGATCTTTTCACGTGTTTTCTTATCAATTTTTTCATATTCTAAGATATATGATAATTCTTCAATCGAATGTTCTTCGTACGGTTTTTGTTCATCAATATCATTAATATCGAAAATAGATGTTTCTTGTACTTTTGAACGCTCAAATAACTTTTCAAAATCACTTTTTTTATCACTCATAATTTATTACACATCCTTATAAAAATTTTATTAATTTATTACCTAATATTATAAATAAAATTAGGTAATGGCTTATATTTTTATTTAATCAACAACTATTTTATTTTTAATTAGTGCATCAAGAGCACTTAATAAATTTTCTAATTGATCTATATTAGTTAAACTACAACCTGCTGCCAAATCATGTCCGCCTCCATTAAATTGACTAGCAACCAAACGTACTTTGTACTTATTACTTCGTAATGAGACTTTATAAGTATTTGTATTTTCATCAAAATATACGATGAATCAAATGGGATAATTTTTAATATTACTTAATAAATAAACTTTATCTCCTCATCTTGTAAAATGATATTTTTGATTTCATTGCGGATCAATGTTTAAGTATGCAACTTGTCCTTGTGTTTTGATTGTTTTAACCAATTCATTATCAAATAAAATATCATTTAAATTACGTTCATACAATTGTTGATAAATTTGATAATGTTTGACATTATTTTTAAAAAATCAAGACATAATCTCATATGTAAAATCACTTACTTTATCGTACATCAAACGATTTGAATCAGTAGTAATACCTAATAAAAGATAATTTAAAGTTTTTAAATCAAAATTTAAATTAGTATGCATTAATGAATATGCTATCATTTCACAAGCTGCAATTGATTGATCATTAATGTATTCAAGATCAGTATAAGTTATTGTTTTTACATGATGATCAACTAAAATTGTTTTTTTTGCTAATCTATGTTTTTGTGATAAAACTCTTTCTTGATTAGCTGTATCAACAATAAAAGCTATTGAATCTTTAATAAATTCATCATCAACTTCATTGGGATCAAATGTAAAAAAATTTTTAAAATCATTAGGATTTAATGCGTTAACCCCAACAATTTTAACTCTTGTTCCAAAAGTATTTAATTTTAAAATTCGTGCTAATGCGAAAGCAGAACCTAAAGCATCACAATCAGGATTTGTATGAACAAAAATAGTGATTTTATTAAAACCATATGTTTGTTCAATTAATTTTGTTAACAAATCTTTTTGCATAATAATTTTCTCCCATTTTATGATAAATTAATTTTAGTGATTTCTTTAGTGTAAACTAATTCATAAATTGCATCTAAAACTAATGATTCTTCTTCTAATTTAGCAGCTGTTTGAACATTAGGATTGGTTGTTGGATTCTTAGGTGCAAACAAGCTACATGAATCATCGTATGGCAAAATCGATAATTCATAAGTATTAAACCGTTTAGCTAAACTAATTATTTCTTCTTTATCGTAAGCTATTAAAGGTCTTAAAACTAAAAAATTATCTAAAACACTACTAATTGTTTGCATGCTATTTAAAGTTTGTGAAGCAACTTGTCCAAGTGCCTCCCCAGTTACTAAAGCATTTGCTTTAACACTTATTGCTAAACGTTTAGCAATACGCATAAAATAACGACGCATAAGTGTAATTCGATAAGATTCTTTAGAAATGTGCGCAATTTCGTCTTGTAATTTAGTGAAATTGCATACATATAGATTCGCTTTTGTTAATTTATTATCTAATGTTATTTGTTTTGCTAATGCAATTGTTTTATCTAAAGCTTTTTGTGAAGTGTGCGGCGGAGTAATAAATGTAATAAAATCAATACTAATTCCTCGTTTCATAATTAAACGAGAAGCAACAGGAGAATCGATTCCTCCAGACAGTAACACTAAGGCCTTACCATTAATACCTACCGGCAATCCTTTAGCACCCATAATTTTATGACCATAAACTATAGCACAATCATATTTTATTTCAATATTAATTAATAGTTGTGGACGATGAACATCAACTTTTAAAAGGGGTGAATTTTGCAATAAATAAGCAGCGCACATTTGTTTAATTTCCATTGAATTGTATTTAAAACTTTTATCACTACGACGGGCTTCAATTTTAAATGTTTGTTCATAATCATTTATAAATTCTTTACAAGCTAATTGAATTTCACTAAGTTCTTTATCAACTTGATAAGCAATACAAATAAAAGCAATTCCATAAACTTCTTTCAAATCATCAATAATTTGTTTTAAATTATAATTTTCTAAATTGATAATTTTCAAATTATCATAACCAACACAATATGTTAAATTTTGATATTGTATTAATATTTGTTTGATATTGTGCACTAAAACTTTTATAAATTGTGTTCGATTTTTACCTTTTAGTGTTAATTCCCCATATTTAATATAAATTATTGGTTTCATATTTATTGATTCACACCTTATATCATTCAAGATCTTCTTTAGTTAATTTAGGACTAATACCTTTTATAATATACTCTTCGTATCATGTTTTTGCTTTTGAAATAACTTTTTCGAATTCATTTAGATCAAGCTCATAATTAACTAAAATAATTTCATTTTTATTAATATCAAATGCTTGCGGATTAATATAATCTTCACTTGTTAAAAAAGCAATAGCAAAAATTCCTTTACTAATTTTTCTTAAATAACAATACAATCCTAATTGTAATTGATATTCATCTGAAATAATAACTTCACTTTCATCATTATTAAATCATTTTGCACGTTTATCGCCTTTCTTTTTTATAATTGGATGATTATGTTCATCTTTTTGTAGCACAAGAACATAATCAATTGTTTTAAATGAAAAACTATCAATAGATGTTGTTTTAATTTCTAACATTGGTTGATTTGGGTACAATAATTGACCATTTTCATCAATTGGTTCACCATCCGGAATTCCACCAAAAATTTGATTATCTTTAAACACATCATAACCAATTTGCGCAGGTTCGTATTGTTTATATTTAACTTTTAAAACAGTCTCAACATAATCTTTGATTTTTGGTTCAATTACAACACCGGCTTGAGCGTATATTGGATCGATTGGTTCATTATAAATTTTAACCATCATTGCTCAGGTACGAACTGGATTAGTAAAATCACCAATATTCAAAATAGAACCTAATCTACTACCTGTAATTTTTGCAAAATATCGTTGATAACGTTTTTTATAATCGCTTGATAAAACAATTTGGTTATCAACTAATCCAAAATCTTTATTTGGCGTTTTAATAAATGGCATAAACTAACTCCTTATTTTAGATCCATTAACTTTAAATTATAATTTATATAATTATTTATTTTTTATAATTATGTATAAATGAATAATCATATTTAATAAAAAGAAAGTTCAAAAATGAAAACAATAAATTTAAAAAAAGAATTTAATCAATATTGTATTAGTTTAAAAACTCAAGATCTTATTAAACAATACTTTAATGATGAAAAATTAAAAGAAAAATTATTAAAAAATCGTTTAATTGGATTAATTAGTTTAATTATCATTTTTATAATTGGTGTGGGAATTTTAATTCTTGGTGCTTTTATTAATAAAGGTAATGGCGCTAATTTAAATAATGGGGCAATAACTTGTGTTATCATAGGAATTTTCTTTTTGTTATGTTTATTAATTCCTTACACTATTGTTGCAAATCAAAATAAAAAGATTATGCATAAATATATTGAATATCATTTTAATGAAGTTAATGTGGGACAACTAATTAAAAAAATAAGTACTTTTTTTCAAGAATTTAGGTTATTAACAGAAGGTTTTTTAAATTTTAAACAAAGTAATCAAGTTATTGATATCAATTATTGTAATGAAAAATATAGTTTAATTTATTCATTCCAACAAAAACCACAAGTATGATTAATAAAAAGCACTAATGGTAATAAAATTAGTTCTAAAGAATGAGACTTAAAAGGAAATTATGATTTAAAAATTTATGAGTTAGAAAAATGAAATGCTGGTTTTTCTAAGTTTTATCTTGAGAATAACATTGAATCAATCGCTTTTCAAATTGAATCAAAACTAAGTACAATTATTAATGATTTTGAAGAATTATGAATTAAAGGTGGAGGTTTCCTTAATGAAGAATACTAAAACATCGCAAGAACAAGTAATAGAATTAAAATTAAGTGATCGGCAAAAAAAAGTATTAAAATCTATCGTCGATGAATACACTATTAGTGCAACACCGGTCTCTTCAAAACTTTTAGTACAAAAAGAATTCAAAGATCAATCTTCTGCTACAATTCGTAACGAAATGATGTTTCTTGAAAAAAACGGATTAATTGAAAAACAGCACATTAGTGGTGGGCGTGTTCCATCACTAAAAGGTTATGATTTTTATAATAAAAATTTAATTAATAAAAATAATAATGTTTCTGATAATTTTAAAATGCGTTTACATAAAATCCTTAGTAAACGATATTCCAATATTGATGAAATTTTAAATGCTGCTGTAAGTATCATTAATGAAACAACCCAATTACCAGCTGTTGTTACAAAAACAAGTAGTGATGAATTATTAAAACGAATTGATTTAGTAAAAATCAATGATAATTCGGCGTTAGTGTTAATTGTAACTTCAAGCGAAAACATTTTAACTCATTCAATTAAGCTTGATAAAAACACAAAATTTAATGATTTACAAACGTGTTTTAGTGTTTTAGATGAACGATTAGTTGATACTAAACTTAGTTTAATCAGTTCTAAATTAGATTTAATTGTCGATATAGTTCGTAATAAACTTGAAGAAGTAGAATACTATTTTAGTAAAATTGTACATCGTGTTTTTGATTTTTACGCTTATAAACCTAACATTGATTCTAAAACTTATGGAGTTAAATACTTAACTAAACATTCTGAATTTGAAGATCAACAAAAACTAAATGATTTACTAAATTTATTAGAAGACAGTACAATTTGACAACAAATTGCTTTAAACAAAAAAACTGATGGGTTAGCAAAAATTATGCTAGGAAATGAAATTGGCCACGAACATTTAGCCATTGCAACAACTCAAATTAATTTAGCAAACACTAATCATCAAATTACCGTTGTAGGTCCAACAAGAATGGATTATGCAAAAATCAAAGCTTTATTAGATTTTTTAAAAATTGAAATTGAAAAAATTTTAGGATCAACAAATGAACATTAAAAAAATTAAACGTGAATTTAATACTCATTACGAATTGATTAAAAAAGAAATTAAAAACTTAAATGAATATAAAAAAGAATTAGAATTCTTAATTGCAACAATTTATTTTAATTCTAAAATGGTTTTTAAAGAAATATCATTAGATAGTTGAAAAAAATTTAGCGATTATGTTTATCGATTTTTTTTAATAATTAATAAAAATAATCAATCACAAAATTTTGATTTAGATTGTTGATACTTAGAAATTAAAGAATTAATAAAAGTACTAAATATTAGTAATTTTTTATTGAATAAGCAATTGGAACAAATATATTTGAAATACATAATTTTTTATTGGTATAACATTAATATTCCAATAAATTATGAAATTGATGATTTTAATATTATTTGTTTACGCAATAAAGCATTTGTAGATTGTATGCAAATTTTACAAGAATATTATCATATTAATGTTGATATTTTAATTAATTTAGTTATCAAACAAATTAGATAAAGGATAAACATATGCAAGAAAAAAAACAAACAAAGAATGAAAATTTTCAAGATCTAGATCAATTAAAAATTGGTGATGAACTAATGGTGCATGCTTATAAACTTAATGGTTGATTGTATCGTTCTTGAAATAATCCAAAAGTTGTTTATATTGATGATCAATTATTAATTTTATCTTCAACTAATACATTAGTAATTACTAGCGAAGAAAAATCTGTTCGTAATTTTCCATCATTTACAAATAAGATGAGTTATTGGTTCTTTTTTAAAAATGAGTGGTTTAATTTAATTGCAACGGTTGAAAATGATGGTATCAAGTTTTATATTAATGTTGCATCTCCTTTTATTTATGAAGAGCAAGCAGTTAAGTATTATGATTTTGATTTAGATTTTAAAATTTCATCAGATGATAAATGAAAAGAAGTTGATGTTAATGAATTTATTGAAAATGCCAAAAAATATCATTATTCAGAAGATTTGATTCAAAAAATTTTAAGCGTTGAAACTAAAATTGAAAATTATATTAAAGAAGGTTATTTTCGTAAACTTGTCACTCGTCAATTATTGATTAAACTACACGTTTTAGATGATATTGATGATTTTAAAACTAATTTTAATAAACACACTAATGAATGAAATTATAATTTAAAAAAACATTTTAATAAAAAAAATGGTTTGCATCATTTTAAAAATAAAAAAGGGAATGTTAAAGATAATGAATAATAATAAAAGCTACATTAAAAAATATTTTGATGATATTTTAATACAGGCTAAACAGGTTTTAAAAAAACATGATTATGCGTATGCATATGATTTAATTTCTAATGAATTTAATAATCCATTAATTGATTTAAAAACTTTACAAGAATTTGAAGCATTTGCTATAGAAATTAAAAAAAGTGCCGAACTTAACTTTATTGATGAAAATGAGGCAAAATTAGCTAAAACAGATTTTTATTGTAAAATTCATGATCCAAAAACAACTTATGTTAGTTTAGCATATTTAGAAACTTTTTTAATGCGTTTCATTAATGAAATGGATGAATTAGATATTACATTTTTAAATAGCTTGCTAACAAATAAATTAATTAATGGATCAACAAAATTAGATATTTTAGATTTATTAGCAACTAATAATATTGATCAAAATTTTAATTTTTACAATAAATATTTAAAACAAACAAAAAATGTTAACCCTGTTAATTTTAATAACCATTACCAAGAAATTGACCAAATTCAAAATTATCTAAATAAGTATTTAGCTAAAAATCCTTCTTTATTACATTTAGCCAATAAATTATTAATGATGTACATTACATATTATTTTCCATTTAAATTTGCACATAATCATAATTTAATTGCTAAAACAATTATTGATTATGTAAAAAATGCTATGGAAAATCATGTTTGTCAGTATAGTGAAGAACAAAAAGCTATTGTGGCTTGTATTAATAAAATTTTAGAAGAAGAGCAAGTATAAAGTTATTTAAATTTGTTAAAATAAAATAAACAAATAATTAGAATATTTTAGGCAAGCATTTTGAGATCAAAATGAAGTATATTAATTATTATGTTAACATAGTGTCTCCAAATACAATTTGGAGTTATTTTTTCATCTTAGAGGATATAAGTCGTGCTTAAAAAAATAAATAAAAAATCTTATTTTCATCATTATCATTCACAATGATTATTATTCAAACTACTATAGTTGCAACAGCTTGTAATAATAAAACATGAATTACGAAAAACTCATTTGAGTTTAGTGAGCAACAAGCAACAGAGCGATAAAAACAAATTCTTGATTCAGCAAAAAACAAAAATCCAACTTTTCACATCAACGAGTGAAATGCATCATTTGCATATTTACAACAATTTAATGCAATAATTGGTGAAATAGAAGCTAAAAAAGACAATTTAAATAATCAAAATTTTTCTAAAGCTAAAACTAATAAAATTATTACTTTTGTTCCTTATATTGAACTTGATTCTAATAAATATTTAACAAACCAAGCAATTTCTAAGGATACAAGAGTTAATTGAATATCTGTTATTAACCAATATGCGAATAATAATGAACATAATTTAATGGCAACTAGCAATGATTTACTAATTTATTCTAATTTAAAACAAACACCTAAATACCAAATAGATAATAAAAAACTAAAAGAAGATTTTGTTAAAAATAATAATTGACTAAGCGAAAATGTTGATTATCATTTAAGTATTTTTAAAGATATTAAATATGATTTTTATCTAAGCCATTGGTGTTATCAATATTGAATTGATCAAAATCGTTTAGATGTTATTTTAAAACTAATTTATAATGCTAATAAAATAATTTTATACCCGAATGGAAATGCTGAATTATATTATGTTGCAAATTATTACGCACAGGCAATTAAAAATTTAATTAGGGAAGATCCCCATTTATCAAAACAAAAGATTAATGATCATTTAAATAATTTTATTAATATGAAATCATTTTTAGAAGCAAAAAATTATTTTAGTTCACAATTAAATAATAATAAAGATTGATTAACAGTTTTTGTCTTAAATAAGTTGTTTAAAATTTTTACAATTGGTGAAAGTTGAGTCGATTCTAAGTACTTTAGTTTTAATAAGGAACTAATGCCTAAATCTTATAAAATTCGCACAAATTATGCAAGCGTACTAAATTCACTAAATTTATCATTAGATGCTAAAAATCAATTTAAAAAAAAAATTTTGAGCACACTTTTAGAATATACAATAAAACATTTTTAGATTTTATTATTGATGGTAAACAATACTATGATCCTAAAAAGCAAAACTTAATTTTTATGGGTTCTTCACTATTTAAAAATAATGTTTTAAAAGATCGTAAAATTGCTAAACAAGAATTACAACAATGAGCACTAAAAATTAAAAACAAATATCCTAATGCTAATTACTTTTTTAAATTACACCCTATTTATAATCAAAATCAAGTATTAGATTACATTCAAGAAATCACTAATGGAATCTTTAAACCAATTATTTTAGATGCAAAAACACCATGAGAAAATATCTTATTAAAAGATTATTTATCAATTAAAAATAACAAAGCAGTTTTATTTAAAAACAAAAACACACCTAATTTTTCATTATGGGGTTTTCAACCAACAACATCAGCACTTCTAACGAGTTTTGATTTTATTCGTAATAATACTAAACTAACAAATGATGAAGCCCAATCAATTATAGGTTTTGATAATTTTTTACTTGGTAAAAATTATGACATTTTAAGACGTGATTATGAACCAAATAAAGATTATACAAACGAAAATTTGCAAATTTTAGAAAGAATATATGGACAATTAATTTTTGCTAAAATTTTAAAAAAATTCTTTTAATTATCAAAGACAATTAAAAGATTATTTAAATAATTTTAAATTATATTCTTTATTTAGTTAATATCCACATTTATTTCTAAATCCAAAATATCACAATTTAGATTTTATTATTAATAATCAATTAGTTTAAAAACTAATAGAAAATTAAAAACCATTTATAAGTATTTTTATAACCTTATAAATGGTTTTTATTGTTTAATATATAATTAAATTATTTTTCTTCTTCAACTTTCATTTGCTTTAAATATTTACGAATGGCATATTCAACTTGCAAATTATTAGTAGCATTTTCAAAGAAATCATGGATGGTACGAACATCATCAAAATCTTTAATACCATCATCAACAAATCATTTTACAAAACTAAATGTTTCAAAATCATTTTCTTCCAAACATACTTTTGCCATATTGGCTACATGCTTACGAACTTTTAATTCTGTATCTAAAATGTGTTTTATTAATTCCTTAGGATTCGCTGGCTTAAAATTAACATCTACACTAAATTTAGTGTGTAATGGAATTTCAACTTTACGAGCATATTCAGAAATTAAATTCTTGTGAACACCTAATTTATCATTGCTTAAATCAGCAATAAATTTTGCTAAAAAAGGCATACTGAATTGGTCATCAGCAATATGAGCATATTGTGCATAAATACTACCTAGTTCTAAATTTAAATTATAATGTGTATTTAAAACATCTATAACTTTTTGAGATTTAACCATTTAAAAATCCTCCTTGTGGCCTAATTCATATATATTATATAATTATTTAACAATTTATAAGAAATAAAAACCTTAAAACTTAACGTTTTAAGGTTTTTTAAATCTTTTATGGTTTTCGTAAAATTAAAGGTTTTTTATTGAATAATTTTCTTCTAAAATAATCATGAGCAACAGCATTAATTTTTTCAACATGATAATAAGTATTAGCTAAAACTCGTAATCCCAAACCAGGAATATCCATACGTGATACACCAAATATCATATCCATGTCAGGATATTTTTCCTTAACTAAATCACGAATTTTAATAACATCATCTTCAATAACTTGTTGATTAATTACAATCATGGTTCCACAATAATGATAGCCATCCATGATTCCAAATGCTGATTCATCATTTTTACGTGGTTGAAATTTTAAATTATCAAATAAAACCAATTTATCATCATAATATATTTTAGTATTTAAATACATTTTTTCGTATTGATAAGCAGACCCATGTGGCGATCAACCAGGGCCAAAACATTCTGTGTAAATTAAAGTAGCACTTGAATCCATTTTAAAATTATTAAATTGTGCGAACTTTCCATCTTCATAAACAATTACGTTATCACTTATATATTCTAAAATACTATTTTTTCCTAACGTGATATTTGTATGTTGCTCAGAAGTTTTTCCATCAACCGTTTTATAAGCTTTAGCTGAGGATTGTGTTGTAATGATACAACGAGCATCATCATTAATTTCAAAATCAGAACGATAAATTTCACCTGAAACATATCCACCACCCATACTAATTGTTTGAAAACAGGGGTTGATTGGATCTTCTTCATCTAAAAATAAAGGTTTAGATGAACGATAAAAATTCGTAAAATAGACAGTATGTGCCATTTTTGAATGAGCTTGATCATATGCTACTTTAATGTATAAATAAGCAGCATAATTTTTAATTTTTTCTTTATTTAAAATCATTTGTTAACTTTATTCTTCTAGTAAAGCTAATTGCAAACGCTTTTCAATTCAATCAGCAACAGATTTTAAACCTTCATCTGTTTTTAAATTCGTTACAAAGAAATCTTTATTACCACGTGATTTTAATGTATCGGCTTTCATTACTTCTACATTAGCACCAACATAAGGAGCTAAATCAACTTTATTAATAATAAATAAGTCTGATTTAATCATTCCTTGACCACCCTTACGAGGAATTTTTTCTCCTTGTGCAACATCAATGATATAAATTGAAAAATCAACTAAATCTGGACTAAATGTTGCAGATAAATTATCCCCACCAGATTCTAAAAATAATAATTGTAAATCAGGGTGTTTATCACACATTTCTTCAATTGCAGCAAAGTTCATTGAAGCATCTTCACGAATTGCTGTATGTGGACATCCTCCTGTTTCAACACCAGCAATACGATCAGCTGGTAAAACAGAAGTATTTAATAAAATTCTAGCATCTTCTTTAGTGTAAATATCATTAGTAATTGCTGCCATACTATAACCTTTTGTTGAAAGGTATCTTGTTAATCTTTCGATTAGCATTGTTTTACCTGCTCCTACAGGTCCACCTACACCAATAATTAATGGTCTTTTCATAATTAATTCTCTTTCTTTTTTAATTTTTAAAAATATTAAATGGTATTTTTATGATTAATGTAATGTTGACTAAAACTTTAATTATGACATGAATAATCGAACAGGTGTGTCTTCGTGTTCCATTTGTGCAATTTCAAGGCCTGGTATATTTTTGCAAAAATCTGTTTTAAAATCCAATGTAAAGACTTTATTAACAATATCATCAAAATAAACATGTTTAAGTTGATAAATTATTTTTTGTCCCTTAACTTGTCCTAATGGAATTGCACGTACACAGTTTTGCGTTAATGCAGCAACTGTAGAGTAAAGATGCGTATATAAAGCAGTTTTTAAATCTATTTTTAAATGCATAGCTAATAAAGCAAACGCAACAGCAGGATTTCCATAAGATTTTTTATTTTTTATTCTTTCAGCATATTCAACTAAAAGTTCACAATTAAAAAGTTCATTATATATCTTTACCATTTGTTGTCCAATTCGACGTTGACCTTCACGAGTCTCTCTTGCTAAACCTTGAAAGTTTATCATTTGATCAATTTCTCAAATTGCATTTATTTTTTGTTTTGGCAATAGTTTAAAAATTTGATAAATTGCCAATAAATCACCATGTAACAATTGTTCATTCATATACAGAAGTAACGCTTTAATTAATGATTCTCCATCAAAAACAATATCTTTTCTAATATAAGTTTCAACTCCAAAAGAATGACTAAATGTTCCAATTGGAAAGTTTGCGTTAGTGATCTGCATTAGGTCTAATAAATTTAAATAGTCACTATTTAGCGTCACTACAGTGTTTAAATGCTTCTTTAAGTTTAATTTTTTTTCTTTCATATAGGGCTTTATTATCTTGAAGGTATTCTTCTACTAAATAGTCGTAGGGAACGATCATTTGTGTTTCAGTAAATTGAGCCGGCATATGACGATTACCTAAATTATGGGCAATTTTTGCCATTTCACCAATTGTGTGTGCTGTAATAATTAATACATCTGATAACTCTAATCTAATAACAACTAGTTTATAATCATCCTTGTATAAGATGTCACCATCCATTAATTTTTTGTCCTCTTCTAAACGAATTCCATATTCAACATTTTGATCTGATGAAATGATAATTACACGTTTTAAAACGTCGTCGCTTGTTAAATGAATGTTCTCAATTTGATAACTTTCAACATTTTTAATGTTAGCAATATTATCTAAAATTTCTTTAAATACAGTCAAGATTATTACCTCGCTTTTTTAAAATGTAACGAAAATAAATTAATATAATATTTTAGTTTGACAATTAAAATAAGAAATATCTTTGTGCTAATGGCGCTTCTGTTAAAGGTTCAGCATCAAGTATATATTTGCCACTTGAAGTCTTTTTTAATTTTTTAGCTAATTCAGAAGCTGATTGTTCTAGTCAATTTTCTAAATCATTAAAATCAACAGCAGCATCAAAAGTTTGCGGATCAACTTCTAAATTTGGAGTCGCAGAGTTTCATTTCATACTCTTTTTATTTACTGAACGGCAATTTTTAACTGGTAATAATTCTTTTTCTAATTTATATTCCTCTTTAATGCCATTTTCTAACCCAATTTTTGAAACAAAACTTACTGATGTATTAGTTAACAAACGTCCATAAGTTCCAAATTGGTCACGCATAATTACAGGTTCGCATGTTGGAATTGAAGCATTTGGATCACCTGCTACACAACGAGCGATTACACCCATTTTTACAACATAATAAGGTTTTGCACCAAAGAATTTAGGTTCTCAAGCAACAATATCAGCTAATTTACCAACTTCTAGTGAACCAATATAAGAATCAACACCATGTGCAATAGCTGGGTTAATTGTATATTTAGAAATATAACGTTTTACACGATTGTTATCACTGAATTCACTATCACCTTTTAATGATCCAAATTGTGCTTTCATTTTGTGAGCCATTTGTCATGTACGAGTTGCAACTTCGCCAATACGTCCCATAGCTAATGTATCTGATGACATAATTGAAATTGCACCCATATCGTGCAATAAGTCTTCAGCTGCAATTGTTTGGCTACGAATACGTGAATCAGCAAAAGCAACATCTTCTGGAACCTTAGGATTTAAGTGGTGACATACCATTAACATATCTAAATGTTCAGCAATTGTATTTACTGTATAAGGAATTGTTGGGTTTGTAGAAGCTGGTAAAATATGGGCATATTTAACAGTTTCTAGAATATCTGGAGCATGCCCTCCACCAGCACCTTCTGTATGATAAGCATGAATTGTTCGCCCTTTCATAGCTGCAATTGTATGTTCTACAAATCCAGCTTCATTTAATGTATCTGTATGAATAGCAACAGCTACATCAGTTTTATCAGCAACTGTTAATGCTAAATCAATCGCATTTCCTGTTGCCCCTCAGTCTTCATGGATTTTAAGTCCACAAGCTCCAGCAGCAATTTGCTCAAAAATTGGATCTTCCATACCTTGACCTTTAGCTAAAAAACCGGCATTAATTGATAATCCATCAGCTGCTTGTAAAGCTGATTTAACTCAAAATTTACCAGGTGAAACAGTTGTGGCTTTTGTACCATCATTCATACCTGTACCACCAGCAATAACAGTTGTAATACCACCATCTAATGCAACAGGAACTATTTCTGGTTCTAGTCAGTGAACGTGAGTATCTAAACCACCAGCTGTATAAATTTTACCCTCACCAGCTGAAATTTCAGTTGAGATACCCACAATCATATCAACATTATCTGTTAAATGTGGATTTCCAGATTTACCAATCGCAGCAATTTTTCCATTTTTAATACCAATATCTGCTTTATAAATACCTGTATAATCAACAATTAGTGCATTTGTAATAACTAAATCCATTACTTCAGCATTACCTAATTTATCATCTAACTTCATAGTAGAATTCATTCCCATACCTTCACGTAGGGTTTTTCCACCACCAAAAACAGATTCTTCACCATAAGTAGTTAAGTCTTTTTCAACTTTAACTCAAAGATTTGTATCTCCTAATCTAACGCTATCACCAGTTGTAATACCGTATAAATCTGAATAATTTTTTCTTGAAATTTTAAACATAATGTTCCCCTTTTTATCTATAGTAGAAACTTGTAAAATAGAGTTTTATTTTTTAAGTTTTCCATTAACTAAGCCATTTACACCTCAAACTTCGCGTGTTCCGGCTAAATCAATAATTGAAACTTCTTTTTTATCTCCTGGTTCAAAACGAATAGCAGTACCTGATGGAATATCGAAACGTCGTCCATAAGCAACTTTGCGTTCTTTATCTTCATTTCCTTTTTCATCAAAAAATACTAATGCACTATTCACTTCAAACAAGTGAAAATGTGATCCAACTTGTATAGGACGGTCCCCAGTATTTTTAATACTAATTACTTTTGCCTCTCTACCTTCATTCATCACAATTTCACCACTAGCGAAATTAATTGCCCCTGGTACTAATTTACCTGGACTAAATTGACTTGATGATCCTGACATATAATCATTATCTCCTTTTATAAAAAAATTTTACGAATTGTAAATGTTATTTGTAAATTGGATCGTGTACAGAAACTAGTTTAGTACCATCAGGGAAAGTAACTTCAACTTGAATTATACTAACCATTGTATCTACACCTTCCATAACTTGATCAACACGTAGTACTTCACGAGCAGATTGCATTAGGTCAGCAACTAGCTTACCATCTCTTGCCCCTTCCATTACATGATCAGTAATTAAAGCAACAGCTTCTGAATAGTTTAATTTTAAACCTCTAGCTAAACGTCTTCTTGCAACGTCAGCAGCAACTGTTATCAATAATTTTTGGACTTCTCTTAATGATAGATTCATTTATTTACCTCTAATATATCTTGTATATTAAGTAATAATGCAATTTTATTTTAACGCTTTTAAAACAAAAAAATACACTAAAATTAAAAAAAAGTGTTTTTTTTAGGTAAATGTAAAATATATAGAATAAAAAAATGTGTTATTCTTATTGGAGAATTAATAAAAACAAAAATAATAAATGCTTTAATGATGAAATTAAAAATAATTTGCATTTATTTTTTTATGGGAATACAAGATAAACTTCTTGTAGATAAAATGAAATTCTAACTTATAATTTAATTTTTTAGTTTTGGGAGTAAATATGAGTTTTTTTAAAAAAAAGAATGATTTACAAAAAGAAGAACACAAACACGTTAGTGTTGTAGATATGAATGAAATTTTAAGCTATGAAGATAGTGAAGAATTTAAAAACGATGAAATTGATTCTAATGAGCCAATTTTTAAACTTAATAATGTTTCATTAGGTTACGGAAAAAAAATGATTATTAAAAACCTAACGGCAACAATTCATCGCAACGATTTTGTTGTTGTATTAGGGCCTAATGGTTCTGGTAAATCCACATTAATCAAAGGATTGTGTCGTATCAATAATCCTTCAAGTGGTTATATAATGTATAGAGATAAATTGATATCAAGACCATTATTACCATTTCTTTGATTAGAAAAAGCATGATCTTCTATTGTTAATATATTTACAAAAGATCGTAAAGAAGTTATTGAAACAATTGATTGACATATTCAAAATTATAAAAAAATTCATGCCTATAAGTCAAAGGAATTAGCTTTAAATTTAGCATATGTCCCACAACTTGCTGTATTCCCAGAAGCTACATCAATTTATGATTTTGTTAAAATGGGTCGTTTTCCAAGTTCAAATGCTTTGGGAATTAATACAAATACAGAACGTGAAAAACAAATTATTGATGAAGCTTTGAAAAATGTTGGTATTTATGAATTCCGCCACAAAAATCTTGAAGATTTATCAGGTGGTCAAAAACAAAAAGCATTAATTGCTTTAGCACTTGCTCAAGATACTGAAACAATTGTTTTAGATGAACCTACTAACCACTTAGATATAAGAAGTCAATTAGAAATTATTGAACTTTTACATAAATTACACCACGAAATGAAAAAAACAATTGTTTTAGTTATTCATGATATTAATAATGGTTTAAAATATGCACATAAAGTCATGATTATGAAAAATGGTGAAATGGTTCGTTATGGAAAATTAAAAGAAACAATCGATCATGAAATTTTATTAGATGTTTTTGGTGTTGAATCAATTATTGTGAAAAATGAATCGACATATCCACAAGTTTCTGTAACAGATTTTAGTTTACCAAAAGACTATAAAATTAATGAAATCAAAGAAAACGAAAAAACTAATTCAATTTATAATGATGGTGATGAAGTAGAAATTGAAAATGAATTAGAAAAAGAAGTTGATAAAACAAAAAAATAAATCAAAATTAAAATAAAAACCGCATTGAAGTGGTTTTTATTTTATTATTAATTAATAATTTATAAAAAAGGTAAGCTTATAAATGAATCAAAGGTTAGATCATTATCTGAAAAACACTAATCAATGTGAAACACGATCCAAAGCAATTGATTTGATAAAACGTGGACGTGTGTTTGTTAATAATTTTCAAGTATTAAAACCCTCATTTTTAGTTAAACAGACTGATTCTGTAGAAATTAAAAATGATGAAATGTATTTCGTTTCTAAAGGTGGATATAAGCTTTTAAAAATTATTAAAGAAATTAATTTAATAATTAAAAATTTTGTTATAGCTGATTTAGGGAGCTCAACAGGAGGTTTTACTGATTGTTGTTTACAATTGGGTGCTAAAAAAATTTACGCAATCGATGTTGGTGTTGACTTATTACACCCTAGTTTAAAGAAAAGTTATAAAATTATTAATTATGAAAAAACTAATGTTAAAAATTTAGATAAATCTTATTTTTTAGAAGATTTAGATTTAATTGTTGGTGATTTATCTTTCATTTCTTTAGAGCAAATTTTTCCAATAATTAAAAAAATTTCATCCCCTAAAACTTTGCTTTTATTGTTGATAAAACCCCAGTTTGAATTAGGAAAGGACGTAGCTAGTAAATATAAAGGTCTAATTCAAAATCAAAAATTACAACAATTAGCAATAAACAAAATAATCAATTTAGCAAAAAATTATGACTTTGAATGTAAACATTTAACTCCTACTGATATTTTTGATGAAAAAAAACAAAATCAAGAATATATGATATTTTTACAAAAGCATGAAAAATAATCATCAAAAAATCATCATGCATCTTGATATCGATGCTTTTTATGCTACTGTTAGTGAACTATTACATCCTGAATATAAAAATTTTCCAATTGCTGTTGGTTCATTAAATTCACGCACAGGTATTATTTCTTCACCTAACTATTTAGCACGTTCTTATGGCGTTAAAGCTGCAATGCCTATCTTTTTAGCTAAAGAATTATGTCCAAATTTAATTATTTTACCTTCTGAACACAATATTTATCAATCTTACTCAAAACATTTTTTTCAAATCATTAATAAATATACAAATAAGATTGAAATAACTTCAATTGATGAATGCTTTATTGATGCAACAGATTTAGTTAAAAAATATCATAATAATATCCGCTTGTTAGCAACAAAAATACAAAAAGAAATCAAAAACAAACTAAACCTTAGTATTTCAATTGGAATTTCATATAATAAAACAATTGCAAAAATGGCAACGGAATTAAATAAACCTTCTGGTATATCAATTATTGATGAAAATAAAATTATTAATTTAATTTATGAATTAAATATCAATAAAATTCCCTATATTGGCGAAATAAAATCACAAGAATTATATGCGATTAACATTTTTAAAATTAAAGAATTAATTGCAACTGAAAATAAACAAAAAATTTCATTAGTTTTAGGTTCAATATATCAAAATTTAGTCAATGATTTAAAAGGTTTAAGTAAAATTAAAATAATTGATGAAGATATTTATAAAACAATTTCACATTCGAAAACATTTAACGAAGATTTGAATGATTTTTATGAAATTTCTAATGAAATGAACGATTTAATTTTAACTGTTACTAATCGATTAAAAAAATGTAATTTAATGACTAATAACATCAGTATTTATATTAAATATCCTAGTTTTAAAACAAAAATTAAGCAAAAACAATTAACTTATTACACTGATGATTATCAAACCATTTTTCTAGCAATTAAAAACTTATTTAAAATAATGTATAAAGATGAAACAATACGTTTAATTGGTATCTCACTAAATAAATTAGTAAAAAAAGAAAATGTTAAAAAACAATTATTTTTATTTGATTAATTGATTGTTTTTTAAAAATCTATTCCTTCAATTGTAATTTTTCGACCTCTTAAATTCTTATTTATAAAATGATGTTGAATTAAATAAGGTTCTATTTTTGTTTCAATTGTATATTGATCTAAACGTAAAATCTGTGCAATTGATTTTAAACCAATTTCATTATCTTGTCGATATAAAACATTTAGATAATTAATATCTTGTTCATCTAATCCAAACTCATAAATTTGAATTTTTTTAAAAATACTTTTAATATCATTAAACCCATTAATTTTAAAATCTACAACTCGTTTTAACAATCGATTTGCTAATCGTGGGATTCCTTTAGCATTATTAGCAATCAAATCAATTTCTTCTTCGTTTAAATCAAGAGGTTTTTTAAACTGTTTTATAATTAATATGTATAAACACTTATTATTTGTTTATATTATTAAAATTTAAAGGAGCATATTGGTTTATGATTTATAAAGAAATTGTACGTAATATTATTAAACATCCATTAATTTTACCAGATGGAAAACACTATCATCTACACAAAATCTTAACTTCAAAAGATGAATTACGTAAAGGCGAAGTAACTTTAGTTGCAGAAAATGGCAAAGAATATACAGTTGATTTATCTGCCTTTGTTGATCTATTAGTTGATGGTGATTGCATCTTTGATGATAACCACTCATTAGTTGCATTATATTTTGATGATGCAAAATAATTAATAATATAAAATAAAAAATATCTACTTTAGTTTTTGTGCTAAAGTAGATTTTATTTTTAAAATAAATATAATCACTAATTATTTAGTAGACAAATAAGTTGATCATAAATAAAATTCACTAACACTAAAATCTGCTAATTCTTTAGCATCATCAATAATACCCATTTTTACTAAGTATTGTAAAAATTCGTTTTTTATATTTTGCATTCGGGTTTTTAATGATTCATTTTTAGTTTTTAATTGATTAATTTCTTCATTATTTTTATCTTTGTTAATACTAATTTGTCATTCGTTTTCATATAACTGTTTTAATAAGTTTAAATAACGATCTTCATAGTGTGAATTTATAGGATTAGTTTCTATTAATTCATCTTTTTTAGCTTTAATGTAATCAGTAATATCATAATCTCGTCAAAGCATGTTAATAATACTAAAACGAATTTTATCATTCATTAATTTAGTATTAGATGTACTAATCGAATAATATTTATTATCATATCAATATGCATTTACAAATAAATTGAACTTATTAAGTTGATCATTAATGTTTTCAAAAAATCGTTTTAATTCATTTTTTGTACTTGAATATTCTTTGTTATTTAAAAATGATTTTTCTAAAAAGATATTAAAATCTAATTTATTAGTTATTTCATAATTTTGATCGATTAAATTTTCTTGTTTTAAATACTTAATAATTAAATTTTTATTTTCATCATTGCTTTGATTAAGTTTTTTAAACGCTAAAAATAAAGTGTTGATTCTTGTTTTATCATATGTTTTTATTTTAAATAATATACGATTAACGTTAGAGAAATAATACCTATCATTTTTATAAATACGACTTATTGGATAGCTATGATTAATATATTTACTTGGAAGATCTAAATTAAATTTTTGATTGTAATACTTGTATAATTCTCTAGCATTGTAATAATGATTTATATGAGCAAAATAAATGAAATTGTATCAAGAATAAAAATAATCTTTATTATTAGAACTTAATTTATTAGGATGATAATATTTTTTAAATATTCTTGTTGCTTCATCAAAATAATGTTCTGGATCTCTTAAACTAATATACTTAATAGAATTTGCTAAAAATTTTGGTTTATTATCAATAAAATATGTCACATTTAAAAACATATAATAATCAATTAATCATTTAAATCAAGGTTTGTTTTGGTTATTTTTAACTAATTCAAAATCAACTAAATTACTTATATGTTGGTCAATGTATGCTAGTAATTTATTAACAAATTCTAAATCATAGGTTTGTTTTTGGTCATCTCAAACTTTACCATCATAAACAAAGCTTTTAATATTATTAGCATATAAAACTTTGTAATAATCATTTTTTGGAATATCAATTATTGGTGATAGTTCTTTAGTTTTTGGTTTTTCTTGTTGTTTATTTTCTTTGATTTCTATTTGCTTTGACAATTTATTAGAATTATTGGATTTTGATTTATTAGTTGTACAACCAATAATCAAAGGTGCAATTAAAAATAATGATAATAAGCTAGTTTTAATGAATAGCTTTTTCTTTAATGAATGTGTAACTAAATGACGTTTGTTCATTTCTTACTTCTCCTTTTTGATAAACTAAAATTTTGTATTCGCCTTTATTTAGATTTGGTGCTCTAATTAATTCAACATTCGAATCTGATTCTGAACGATAAATGTTTATAGTTCTTAAAATTTCGCCAAAGTTATCAAAATTTTGATAATCAATATTTTTTAGATCTTCATTTTTAACTAAATAGATATCATAATTGTCATTTTTAAAATATGTTAGTTTTTTATATTCGTTGTATACACTATTATAATCATGTTTTTTTGAAAAACTATTTGATGTTGGCTTAAATAATCATGATAAAGCAAAATTGAAATTAGTTTGATCATTATCAATACTTATATCCATTTCTTTAACTAGTTGTTTATCTTTGTGTTTCGTTAAAATTTTTGTTTGTTTTGCGTTTTTATATGCTTCTATTGCTCTTTTAAAATCTAAATGACCAGCACCATATAAATGACTAAAACTATTTTTATAAAAATCATAATTTTCATTATTTTTTAAAGGAATTGATGAAGCAGCTAAAATTGAGCGAATATGTGTTAGTGGAATTGTTTTATTAGCGTTATTAACCATATAATAAGATTGCAATAAACTAATCGCACCAGTGACAATAGGCGCAGAAAAACTAGTACCTAGATTTATATTTTTATCTCCTTTATTTTTAATAAACTTTGTAATTATTGAAGTGTCATAAATACCTTCTCCAGGAGCAACTAAAAATGGCATCAATAAATCTCCATATTTATTTTTAACATCATTCAAGTGATGTCGATCTGAAAAATTACTTACTTTTTCATTATTTTTATTAGTAGATCCTACATATATTGAATTTTGATTAAATCTATCCATAAAACTATATTTAGATATATCAAATCAATTAATTTCTAAAATATGTCCATTTTTATTTCTATATCGTATTTCATCATTATAAGAATTTCCAGCTGAAAAAATATTTATAATATCAAAATAATATGATATGAAATCATAAATATAATTTTTAAATTTATATTTATTAATAGTATTATTAACAATATCATCCTTATTCAATGTATCTATATATTTTTTTAACTCTCTTTTAAAATCTTTATCATCGTTTATTTCTTTACCATTAATAAAATTATAAAAACCAATATTTAAAAAATCATTTGGACTTCATCCATAACTATGGTTAATAATTTTTACGTCATTTTGTATTAATCAATCTAACTCTTTTCAAATTTTAATATGACTATTTTCTGATGTTTCATCCTTTGCATTAATATCTAGTGAATAAAGATGGACTTTCTCAATTTTCACGATCATTTAAATTCATTAACTTTATTTCAATTGATTCATCTTTATTTATATTATTAAAATTAGGATTTAATGATAAAGTTAAACTTTGTGAAGTACTTTCTTCTTGTGGTTTATTAAAATGAGCATCAAAATAAACTTTTGAAATTAAATCATGAACAGTAATGTTGTTATTAAAAACTACTTGCATTTGATAATATTGTTCATTATGATTGATTAATTCCTCAAAAACTCAAATTTTATTTTCTTTATCTCAATACTCAATACCAGTAAATCGATAATTCCCTGAATACAATTCTTCAAATTTTAAATCTTCATGATTTAAGAATGTTAGGGCATTAACAGGTAATGTTTTAATTACTTTACCATGTTCATGTTGATACACTAATCGCATTATTTTATACTGTAATCCAGATTTTTTTGATAACAAACTTTCAAAATCATCTTTTATAGGATTGGTTTTAATTAATAAATGATCATTGGGCGTTGGAGTTTGTTCAAATAAATTAGTATTATCATAAAAATCTGCAAAAAACTCATCACTTAAACGATCTTCTACATTTTCAATAAATTCTTCTCTTAATTCAAGCAAATCTTTTGAATAAACTGGTTCAAAACTTGTTCCACTACTTGAATCGATTTGACAAAATTCTCCAACATCTTGATCAAATCCTCATTCATTTTTTAAAACTAAATTTTTACACTCACCAAATACAGCCATTGGTTTTTGATCAATCTTTTTATTAAATAAAATAAATTTAATTTTATTAATAATTACTTGATTAATAAAATCACTATTTGATAATGATTCAAGTTGTTTTAAGTCCAATTTATTATTAAAAATAATTAAATCCATAAAAGGTAAATAATCAAATTCATTATATTGATCTACACCTGATATTTTTGCATTTTTAATTCAAGTTTTAATTTTTAAATGTTGCTCAAAAATTTCTGTTTTTGAAGCTTTTGTTGTATTAAAAATCAAACGAATAATTAATTTATCTTTAAAATAATCTTTATTGCTTTCATCAATTTCAATAACACTATAACTAATTTTGTTTTGATTTTGATTGTCAACCAAATTAACTTGTTGGTTTAGTTTCGAACTAATTGTTTGTTGACTTTTATTTTTTGCTAACAAACTAAACGATCCTAAAACCATCGAACTTAAACTTATTGTCGACAATAATATTGCTTTCTTTTTAAATTTCATATTATTTTATCCTTAAAATTATTAAATTTTTAAAATAGGTGTTAAAAAAAGTATTAGTCAAATAAAACTAATACTTTTCTCAATTTAGTGAAATTCAAATTACGAAACGAAAGTATTAGCTCAAAATTTTGATATTAATAATAATTTGACTAATTTTCATAACTAATAAATTCCTAAAAATAATTTTTAATGTTAACGCAATATTATTTTATGTTTTTTATAATAATTAATCATTTAAAATGCAAAAACACCTTTAAATTAATACTTTTTAGTAAATAAAATAACTTTTTTAAATAAATACTATTAAAACAATCTAAAAATGGTTTTGTGTAATCGCTATTATTTACTATTATTTTCAATCTTTTGCGAAGTTCCATTTTGTATTTTTTTAACTTCTTCAATTAGTTTTTTTAGTTTTTTATTATAAAGATCTAACGTATAAGAAGACACATTATTATTGTTAATTCCTTGTTCAACAAATTTAGTAAATTTTTTTAATCTTTCTACTTTAGATTCGTTATTTGTTTTTCCATTTTTTGTTTCAGTAATTTTCTTACTTTCTTCAATTAATTCTTTAGCTTCAGGAATATATAATTTTTTATCAACAATCGTAAAAATCTTAACACTAAAATTTTCAATATCTTTTGTGAACACCCTTTCTTGATTATTATCTAATTTTAAATAGTTAATTAACTCTTTTGGTTTTAATAAAAAATCATTAGAATAAGCTAAATCAATATTTTCTAGATTATCAATTGTTAATGTTTGATTTGCACTAAGTTTTAAACTGACTTTAGTATTTATTCAAGGATCATTTTTATAACTATTTAAAACATCTTGAATTGTTTGGGTTTTATTAGTTTTCTTTGCTTGATCATTTTCACTATTAACTTTAGTATTAAATTCTTTTAGATGTTTAATGAAATCTTTGAATAAATAAGTTTTTTTAAAGTTTGATAGAATTGTTTGTTTGATTTGATCAACAATTGAACTAATTTTAGTATTAGCATCTTCAAATTTTTTAGTTAATTCATTATTAAGATTATTTGCACTATCAATCGTTAAGCTATTTGTATTAAAGCTAAATAAACTAATAATTGATTGTAAATTTTCAATTTGATTTTCTAAACCATTATATGTAGCTTTTGAACTATTGTTTTTGTCTTTTTGGATATTTTGTTGTTTTTCAATACTATAGTAAGGATTGTAATCGTCTGGTTTTAATGATTGAATAATACTAATTAATTTATTAATACTATTTTCACTATCTAATGCAATTGTATTTAAACCTTGTAAATAATTTTGTAAATCATTTAATAATTTATTAGTTAAAGATTTAATTTGTTCAATATCTAATGATTTTTTAACATTAACAATATTAGTGATATTCTTGTTTAGATTTTGTTTTAAATTAGTTAATTTTAATTGATCAAATCATAAAAAACTATAATTTTGTTGGTAAATAGTATTAATAACATTAACAATATCATCTAATTTTTTAATAAAAGGTAAAGCTTTTAGTTCTTTGTTAATTAAATCATTAATATCATTTAATTTAGTGTAAATTAATTTAAAATCTTCATATAGTTTTTTAAAATTAGCTTTTGTGTTATTATTAACTAATTTTTGAATATCACTTTCAATCTCGACAATATTTTTATTTGATCCAGATGTTTTAATTAAATTTTTAATTTTATTAGCTAAATCTAAAATTGTTTTTTGAATTAAAGCTTTAGTGTGCAATCTTTTTTGATGAGCATCAGATTTTAGTTTATCAATTATTGTAGTATCAACACTTGCTTGATTAAGTAATTTTTCATATTGATTTAAAAAATCTTGTAATTGTTTTTTAGTGTTTTCTAATTCAACAACATCTATTAAATCACTCATGTTTTTGATTGAATCTAATTCATTTAAAACACTAACTTGTGTAATTAAATTTTTAATTGTTTGTTGTTCTTGTTTTAATTTTTGCTCTGATAATTTATTAATGTTTTCTAAATTTGATTTAAGAGTTTGATTATATATCTTTTTAAAATCTTCATAAGATGATTGATCAACTATTTCTTTTAATAAAACAGCTTTATTTAAACTTGATTGTAATTCTTCTTTAGCTTTTTGTTTTGTATCAACTTTTGCACAAGCTGTAATTATTGTTGCTAACAAAGGTATAGCACTAATCATACTTAGTTTTAATCAAATTCATTTGTATTTTTTGTGTTTCATATTTAAAACTCCAAGAATTAATTATTCAATTACTAGCTTAACTTCTTAGTTATTTAAGTATTATTCAAATTAAACGTTTAAATATTTTAACATTATTAAAATAAAAATCTACTCTAGTTTTATTCTAAAGTAGATTGATCTAAAGTTAGATATTCTTAAAGTAACAAATCTAATAAATGTTTAATGTCAATAATAAATTTTACTTTATTCGCAACTAGTTGTTCTAGAGTTTTGTTAATACTTGTGTTTTTTAAAAGATCAAAATTAACTTTTTTATTTAAAACAGCATTTGAACTAGCTCCATAAAAATAAAAATCTTTTTGATTTTTCATATTAATATAACAAACTAAAATCGTAATTTCTACTTTGTCTAATTCATCTAATATAGATTATCGTTATTGTTTTTATTTTCAATATATCGTTGATATGATTCAATTAATAATTTTGTTTTTTTCATAATCATAATCACTATATAATTTTTTTACTTCAATATATAAATAATGATCTTTTTGATCATGTGTTTTTATTTTAAAATAAAATCAGGGTATGAATTACTTATTTGACCATCTTCATTAAAATATTGATATTTTAATCCATGAAAAACAGGATTTTTAGTCCAAAGTTCAACATTATTTTTAATTTTATCATTATCATTTGATAATGAAAGAAAACGATTTGCAAATTTAGATTCAGTTGAAGAATCAAAATAGTGATTTTGTTCTTTAAGATCTTTATATGCATACTTATCACAAATGTTTAAATCTAACATGTTTTCACATTTTTTATATTTTTCAATAGGAATAAAAATTTCATTTTGATTAGGTAAATTTTTATTATTTTGTAATTTAAAAAGTAATTCATGATTATCTTTTTTCATTTGTTCTAATGAATTTTTATATAATTGTTCAAATTGATCAAGATATTTTTTGATAATAACATATCAATACATATTAATGCTTATGTCTAATTTTCATCTTTTTTCTCACTCATTAAATTTTATTTCAAGAAAATTTATAATTTTTTTAGGGAATAGATGTTTGTATTTTCTTAATTGTTCAATAATATAAAGTTCAAGTTCAATTGAATTTTTAATTTTAGTATAAACACGTGTTTTGCCTTCAATTTGTGAAGTTTAACCTTCTAAGTATTGATTTTTTACAAAATTTTCTCGATAGTTAAGCAGGGCATTGAAAATTACTTTTTAAATACTTAAACATGCCTAACATTTCATTTTGATAAATTTAAATATTATAACTAGAGATAATTACTATTATGAGAAGATAGGTAATAATAAACCTAAAAAACTTGAAAATCTACCATTTAATATACCTAATAATTGAATTTGAGTAAAACTAAATAACATATCCAATGTCATAAGTGGATATTCTTTTAAGTCATCAAAGTATACTAGTTCAGGTATAAGAATAATAAGAATTTCTGATTTTGATAGTAAAGAAGTTGATAATAATGAACCTATTTTTTATGAATATAATGAAAAATTTAATTCTTACAAAATTGAAAATAACGATATTATTTTAGTCATGACTGGCGGTACAGTTGGAAAAAATATAATTATAAAAAAAGCTAATGATTATTATTTAAATCAAAGAGTTGCTAGAATCAGAACATTTAATGTAAATTATAATTATATTTATTATTTAATAAATACTACTTATATATAAGGATTAATAAACGATTCGAAAAACTCAACTAACGACAATATTTCATTAAAAGATATAAATAATTTATTAATACCATTGCCACCATTAGATGAACAACAACGAATAGTTGACAAAATTAATCTATTAGAATTTTTTATTAAACAATATGATGAAATAGAACAAAAATTATCGAAGCTTGAAAATGAATTTCCAGAAAAGCTAAAAAAATCGGTTTTACAATATGCTATGCAAGGCAAATTAATTAAACAAGATCCAAATGATGATTCTATAAAAGATTTATTAAAACAAATTCATAAAGAAAAACAAAAATTATACAAAGAGGGTAAACTTAAAAAGAAGGATTTAGAAGAATCTATAATTTATAAAAGTGATGATAAGTCGTATTATGAGAATATAGGTAATAATGAGCCTAAAAAGCTTGAAAATCTACCATTTAATATAAATGTATAAAGATGAAACAATACGTTTAATTGGTATCTCACTAAATAAATTAGTAAAAAAAGAAAATGTTAAAAACAATTATTTTTATTTGATTAGTTGATTGTTTTTTAAAAAATCTATTCCTTCAATTGTAATTTTTCGACCTCTTAAATTCTTATTTATAAAATGATGTTGAATTAAATAAGGTTCTATTTTTGTTTCAATTGTATATTGATCTAAACGTAAAATCTGTGCAATTGATTTTAAACCAATTTCATTATCTTGTCGATATAAAACATTTAGATAATTAATATCTTGTTCATCTAATCCAAACTCATAAATTTGAATTTTTTTAAAAATATTTTTAATATCATTAAACCCATTAATTTTAAAATCTACAACTCGTTTTAATAATCTATTTGCTAATCGTGGGATTCCTTTAGCATTATTAGCAATCAAATCAATTTCTTCTTCGTTTAAATCAATCTGAAAATGCATCTGATTATTCTTTAAAATAATTGCACTAATTTCTGATGGTTCATATTCATTTAAATGAAAGATGTGTCCGAAGCGTTCTTCTAAAGATTTTGGGATTTTTCCAAAAATTGTTGTTGCACCAATAAGTGTAAAATGAGGAATCTTTAACCTCGTCATTTTTGAATTAAAATCTTTACCTATATTAATATCAATACAAAAATCTTCTAATACAGAATAAAGTGTTTCAAATGCACCTAAACCACAAGCGTGAATTTCATCAATAAAAACAACGTCATTTTCTTTAATTAAAGATAATAAATTAATAATATCAATAGGTTTATTTAACAAGTTCCCTTGTGTTATGTGACAATTAACATTCATTTCATTAGCAATTATAAGTGCTAATGTTGTTTTCCCTGTTCCTGATAATCCATGTAATAATGTATGATCAAAACTATTTTCTAATCGTTTACTAGCAGTTAAATAAACTTTTAAATTGTTTTTTAATTGTTCTTTACCAATAAAATCTTTTAGATATTGTGGACGAAACTCATAATTAGTTTTCATGGCGCAATGAAATTTCTTTGATTGCAGAGCTTATAATTTCACTAATATCAATATCTTGTTGAGAATTAATTTTAACTTTAGTAATAGCATACTCAATATCTTTTTTAGTATATCCTAAATTTTCAAGACCTATACTTAATTCTTTAGCTCAGTTATTTTTATCATTTACTTTATTTATGTATTTATAATTTAATTGATCACAAATCAAATTAGCATTTCGTTGGTTAATTCCTTTAATTGTTATTAAATAATCTATATTCTTAGTAGCAATTGCATCAATAATTGTATCAATATCATATTTTAGAATATTAATAGCTGTTTTTGGGCCAATTGAATTAATACTAATCAAGTCTATAAATAATAACCGTTCATTATAAGTACGAAAACCATATAAATCTTTAATAATTAAATTTTTATTAGGCATTATGCTAATATATTCATACAAATATAATTTGCAAAATTTATTAATTTCTAATTCATAATCTTTTGGGATACGTATTTGATAACCAATAAAATTATGTTCGCCAACAACAATATTTGCATTTGCATAAATAACTTTAAAAATTAAATAGTTCATTTTAACCACCTCAATAAAGAATTAGTTAAAACTAATTATTCTTATCTTTTTTTATCAACTTTAGCTTTTTTAATAAATTTATATAAATCAAAAATTAAAAAATAACCAAACCCAAACATCCCAATTAAACTACCTAAATAAATACTAAACATTCTTCAAATAAAAATAATATTATTTATATCTTGCGTTGATAATCAATTCATTGCGTAACGAATTTGATTTTGATCATTTGTTAAAAAAACACGTAACATAAATTGAATTGAACCCTCAGATCCAGGCAAAGGGACAAAATTAGTTGCTGTGACAGCAATATTTGTATAATTAAATAAATCAAAAAAGATGTTATGTGGTTTTGGTTCTACTAAACGATATGAAATATACATTAGTGCATAAATACCAATGTTATAGACTACTCCAATAACAAAAGCCAATAATGAAACCCGCAGTGTTTTAAAATAATACAAATAAAAAGCTTTAAAACTTATATTTTGATTATTTTTTTGAATTAATTCAATTTTTGTTAAATATTTAAGCTTAAAAAGTTTTTTGACTCAATTATAAATTAAAACTGAACGAAAATGCAATTTTTTAGAGAAGCCTAAAACAAAATAAATAGCTGTCATACATAAATCAGCAATTATACCAACAACAGCCAAATAATAATAAAAGGGTAAACTTGTTTTATTATTAACTGCATAAGTTATTAATTCATAATTAATATAAATAAAAGATGGTACAGTTACTATAACTTGGCCCAATTCATAAAAAGTTGATGAAACAAGTAATAGTGCAGAAACTTTTTTTAGATTATGATAACCATTTTTATTTAAAAAATAAATTGAAAAAGGTTCACTTCCAACAGAAAAAGGAGTTACAGCATTTAAAAAAATTAAACTAAAACTATAAAGGATTCATTTCCATCAAGCAATTTTGATTTGGTTTTTATAAGTATCAATGACTGCAATAACTATAAACATAAAAGCCCTAAAGAAAATAAAGCTAATAAGAATTAACAATCATAAAATAGCTAATTTAATAGTTTTTAAACTATGATTTATTTTTATAAATAAATTTTGAAAATCAATATCTAAAATAAAAAGAATTGAAATAATAATCATTAATAAACAAACTATTAAACCAATTATTAAAAAACAAATATTCTTCTTCGTTCAAAAACTAGTTTTTTCATTAATATTATTATCCATTTTAAAAATCCTTATTCTAGTAATTTAATTATATAATTTTATTCCTAATAAAAAAGAAGGGCACCCCCTTCTAAAAATTTTACTTTTTTGATGATTTGTTTGCTGATTCAGTTTCTCATCATGTTTTTTTCGAAATTGGATCACTTTGAAATTCTTCTTCAATTAATACTGTTTTTAATAATAATTGTTGTTTTTTATCTAAACCATTATTAATGGGTAAAACATCATCAATTGCTTTTTCTATTTGTTCTTTTCCATTTTTTTTAACAATTAAATCATGGCCACGATGATGACGTTGCGCTTTATGTTTATTATGGTGATTATCTGGATCAATATGTTCATATTCTTCTTCTATAAAGTATTCATTATTAGGAAATACTGAACTTTGAGGTGCTAATAAACATTCATCATAAACTAGTTGTGCTAGTTCAGCTTCATCACGTTTATTTCAAACACTTACATACTTTGATAACCAACTAAAATCATTTTGATCATCATTAATTAAACTTTCTTTTTCAGAAAATAAAGATTCTAAAAATAAATTTTCAGCATACACTTTTTGGTTTGAATAAATATTTTCAAAAACAGGTTCTTCAAATTCAAAAGAAACAGGAGTAAAAGGTGTATGTTCTTTTACTGAATTATCAACTTCATCAACTGGAGCTACAATATTCTCCTGTATAAATTCTTCATTGCTTTGATTTTCGTTCTTTATAGGCTCAGGTTCAACTTCTTCATGTAATGGTTCAATAGGTGTAGATTCATGTTCGTTAACAATATCATCTTCTTGGTTATTAACTGGAGCTGATGATAGATCATTGCAATCTTGTAAAATTTGTGGTTCATCCATTACAGGTATTTGTTCTTGTTGGATGTTTGTTAAAGCTAAATTACGTAAACTTTTAATCGTTTTTGTGTAATCAACTCACATAAAAACGCCACAATTTTTGCATCTTACTTTAACTTTTTTAGTAACAATAGTTTCATCATATAATTGATAAATATCTTTGTTATTATCTAAAACATCAGTAATATTTATATTTTTATCTTCAGTTAACTTTTGATTTAATTTATTTTTCTTACTAAATAATCCCATAATTCAACTCCAAAATTAATTATTTTTATAATTTTAACTTCTTTAACTAACTTAAATTATATATGAAAATAAACTTTTTTATTTCATTTATTAAATCTAATAAGCACGTGCAAATAAAGTCAATTTGTTTGTTTTTTTATTTGTATAAAAACACTGCCCGTTTTGAGTTTCATCAAAAATACAACGTGTTGAAGCATTAGTTAATAATTTAATTTGTTCATCATCTTCTTTTGTACCATCAAAGAATGCCTTAGCCATTTTATTTTGTGCAATAACTTGTTTTAATTCCTCGATATTATTTACAAAAACAATCGAATCCTCTAAATCTTTTTTTGCCTTTTCATAAATATTTCTTTTAATATCTGCTAATAATTTTGGGATGATTAGTTTCAAATCAGTTAAATCAACATTAATTTTTTCATGAGTATCACGACGCACAATAACAACTTGTTGATTAACTAATTCTTTAGCACCAACTAATATACAAATAGGCGTTCCCTCAATTTCTTGTTGTGCTAATTTATAACCAATTCCTTTATCACTATGATCTTCAAACAAACGATAATTATTTAATTGTTCACAAATCTGTTGACTAATTTTTAATAATTGGGGATTTTTATTAGAAAAAATCGTTAAAATAGCAATTTGATTAAAAGCAATATCAGGGGGCAAAATCAAACCCTGATCATCAGCATGAACCATAATTAATGCTCCAATAATTCGTGTTGATAATCCAGCAGACATTTGATGTACATAATGCATTTGATTATCTTGACCTTGAAACTTAATATCATAAGCTTTTGCAAAAAACTGCCCTAAATAATGACTTGTTGCAGATTGTAATGCTTGGCCATCTTGCATCATAGCTTCAATTGTATATGTTTTTTGAGCACCAGCAAAACGTTCTCATGGTGTTTTTAATCCTTTAATAACAGGTATACACAAATAATTTTGAACAAAATCTGTATACACATCTAAAATTACTTTAGCAAAAGAATCGGCCTCATTTTCACTTGCAAAAATAGCATGTAATTCTTGTCAATGAAACTCAGCATTTCGAAGAAATGGACGTGTTGTTTTTTCAGCACGCATTACTGAACACCATTGATTATTTTTAATCGGTAAATCATTATAACTATTAACAATATTTTTAAAATAATTACAAAATAAAATTTCACTCGTAGGACGTATTGCATAAGAATTATCTAATTTTTTATCACCAATTTGATTAACCATAAATAATTCAGGAGCAAAACCTTCAATATGATCTTTTTCTTTTTGAAATTCACTTAAAGGAATTAAAGTTGGCATGGCAAGATTGCGTACTCCGTGTTTTTTAAATTCGACATCAATTTGGTTTTTAATTGCTTCTCAAATTGCTCAAGCATTTGGTTGAAAAACCATCATTCCTTTAATATCAGTGTACTGAATTAATTTTGCATTATTTACAATACTTGTATATCAATCAGCAAAATTTTCTTTTCTTGTTGTAATCTTTTCTAATTTTTTACTCATTTTATCAATCCAACCTAAACTTTTATTAAACAATTGTTTTGTGTTTATCTGTAAATTGCTTTAAACACATTTTTAAAGCACTTGCGCCAATACGGGCACAACGAATACGATTCATTTGTTTTTTTACATTATAAAAAGCAATTAATTCTTGCATAATATCTTTATTGTAATCTAGCCCTTCAATCATATTTAAATAATTATTAATTAATAATAACGCATTATTAAGATCTAGATTTTTTACAAGTTCACAAATAATATCAGTGGATGATATGGAAATTGCACAGCCTGTACCTAAAAAAACGACATCAATTATTTTTTGATGATTTAATTTAACATAAACTGTAATGTCATCACCACATCCTTCTGTTGTATTATGATATGACAAATAATTTATTTCATCTTGTACAAAACAAACTTTATTTTTAGGGTGTTCATAGTGTTGCATGATAATCTTACGTAATAAAAGATTGTCATTGATATTATAAGATGCCATTTAATTCATCTCCTTTTTGATATTCTTTAATTGCCTTAATTAATCTATCAACATCATTAAAATCATTATATATGTAAAAAGATGCACGAATAGCACATTCGGTTTGAATCACGTTATTAATAATTTTTGCACATGATAATCCACTTCGCACAATAATATTTTTTGATCCTAAATAATTAGCAAAATCTTGGCAAAAAACACCATTATAACTAAAAGTTATAGTACTTGAAGTAGCATCTTGCACATATGTTTTAATTTCAGGAATTAAAGCTAATTGGTCACGTGCATATTTAGTAATTTTTAATTCATGTTCATGAATTTTATTATAACCTATATCCATTAAAAATTTTAAAGCAGCATTAAAACCATAAAAACCAGCAACATGCGGAGTTCCACCCTCAAATTTCATGTAATTATCATAGAGCTGATAACTATTTAAATCAATGCTAAAATTCATTCCACCACCATATTTTAATGGTTGCATTTTTTTAATCAAGTCATTTTTAATATAAGCAACACCAATTCCTGTAGGTCCTAATAATTTATGTGCTGAAAAGACCATAAAATCACTTTTGCATTTTTTCACATCAAACATACGGTGTTGAATACTTTGAGTTGCATCAACGCACACTAATACATCTGGTTTAATTTTTTTAATATTTTTAATAATAAGATTTTCATCTAAAACATTACCAATTAAATTACCACCAGAAGCAAACGAAACCACTTTTGTTTTAGGTGAAATTACTTTTAGAAAATCACTAAGTTGTGGAAATTGATTTTTTTGATTAGCAAAAATAATCTTGACACCTAAATCATCTCGAAGTTTGTATCATGGTAATAAATTAGAAGCATGTTCAATATAAGTTAAAATAATTTCATCACCCTTTTTTAAATGAGGTCTTAAACCGTTTGCAATCAAATTTAGAGATTCTGTAGCACCACTTGTAAAAATGATTTCATCAAAATTAGCATTGATGAATTTGGCAACATTTGCTCTTGTTTCATTCATAATTTCATGAGTGTGAAAAGTAAAATTTGAATCAGTATTATGGGGGTTAGTAGAATATTTTGTATAATAATCAACAATTGCATCGATTACAACTTGAGGTTTTAATGAAGTTGCACTTGAATCTAAATAAATAACATCTTTGTTATTTTTAAATCAAGGAAAAAATTGTTTGTAATCATTCATAGCGTATCCTTGCTTATTTTTCAAAAATAAGACTAAATTTCTTGCGATGGCTTGATAATTAAATTTGTTAAAAACAATAACAAAGTTTGCTTTTGTCAGCTCAACTTTAGAAAAAATCTAACATACTTCTATAGTATAATATTAAAATTATATTGTTTAAAACGATAAAATTATAATATATTCTCATAGCAAAAATAATATGGAGTTTGATATGAAATTTAAAAAAATTCTTTTAACAAGCACATTAAGTGTTTTAGGGGTTATTTCAATTGCAAGTTTAGCTACAAATTGTTCACAAACGAAAACAAAAATTAACCAGTATAAAATAACTATAAATAAATTAGATAGTCTAAATTTAACAAGTAATTTAACTACAAAACAAGCTTTAAAAGATGTTTTATTCTCAAAAGAAGGACAAAATGAATTTTTATTATCAAATGTTAAAAAAGTGGTTTATGATTGAGCAAAAAACAATAATGATCCAAAATTAGCTCATGATGTTAGTGAATATAATACACAATTAGATGAACAATTAAAGAGAATTAAAGAAAATGTAAAAAAATCATACCCAAATGATTGACAATCTGTTTTACAAAGCGAACTATATGATACTGAAGGTGGTAGTGAAAAATCATGAAAAGAAGCTAAGTTAATTGATCAAGCATACAAATCAATTACTTCTTCAATTTTTGATGATAATGATAATTGAATTAGTGTATTAAATGATCAAAATCAACTAATTGATGCCTATTCAAATGATGCTACTAAGTTGTTAGAGAATGAAAGTACTTTTAAATCAACTAAAATTGGAGATAGAAACAAAATTGTTGCTAAGACAAATAATTTTTTACAAAAAGGATACGCTAATTTATTTAATTTCTTAATTGATGATTATATTAAATATAACCTGCCTTTATCATTTGCCAACATTGAATTTAAATATGATGATTTAAAAAATCAAAACATTTATAATAAAGATTTTTTTACAAAAATACCAACTTCTGGCACATCACCTAATTTTCCTTGATTTGATCCTAAACCAACTGATAATAAATCAACTAACACTACAACTAATTATCTAGAAGTAGCTAAATTATTAAAAGAAAATAAATACTTAGATTCAAAAGATGATACGAATAATATTGACAAAAAATTCAACAACAATAATGATATTAATAAATATTTAAGCTTAAATGATTTTATTACTCAAGACAACACAAATTTAACATTAAGTGTCGGTTTATTAGCTAAATTTTTATCATTATTTGGAAATGAAAATTTTGGTATTTATCAATTAGATAATTTAGATGAAAATAATATTTTAAACAATTTTATTAGTAAGAATCAAAGTAATGCAAACACAAATAATGTTGATTTATTTTATCCTTTAGTTAAAAAAGATAAAACAGCTTTTTTAAATGATTTTAAGGATGTTAAAGCTATCCGTCAAATTAATGATTTAAAAAATGGATATACTCTATTACGTAATGAAAACAGTGTTGATTTATGAGGTATTAACAATTTTAAAAGACTTCAACAAGCAGCTAAAATAGGTTTGAACGCTCTATTAACAGAGTTAAAAAATGATTTTTTATACAATGGTGCACTATACTTAAATTCACGCAAATCAAATAGTGTTAAAAAACAAGGCTATGATATTAAAGCTAAAATTAAAGATTACTTTAATAACAATAAAGAGCGCTTATTAGCACTATATATTGCTACACATCAAAACGATAGTGATTTTTTGTTTAAATCAAACTTAAAAAATGATTCAAAAGTTTTTGCATTTGATCAAAGAACAATTAATATTATTAAAGAAAACAATGAAATTCATTTATTAAATTACTATGATAATTTAATTGATAAGTTAAATGATAAATTAATTAAATTTAATAAAAAAACATTTATTATTCCTAGTGTTGATAGTTTATATGATAATGGAATTAATACACCAATTTACTTAATTAAAGACCAAAATCAACAAATTAGTTTATTTCAAACTTTAAAAATAAAACTAGTGGGTAATAAAAATCTTATAGATGAAAATCAAAAATTATCTACAATGATTAATGATTATGTTGAAAACTTTGATATAAAAAAACACCTTGTTGCTAATAATAAAAACTCCCAATCAATTGTTTTTGACGATTATGTTATAAATAAAGTATTTAGTAAAGGAATAAACAGTGTTTTTGCAAATATTATTAAACAAGAACAAATTAAAAAAAGTGTAGCAAAATTCTTTGAATTTAATGATTTTAATTTTAAAAATAATTTTCTTGAAAACCAAGAATTATTAGCAGCATTTAACCAAATTATTAAGAATACAAGTGTTTTGGAAACAACACAAACTAATAATTTTAGTAATTTTGATAACTTAAAAAAAGTTTATGAACAAGCTTTGAAATTATGAAACGATAATTATACTAAAAATGGCCCTTTTAATCAGTTATCAATCAATTATCAAAAATACCAAGCATTATTTGAAACACTCGCTATTATTAATTACTTGTTAGATTTTAAAGATGGTAAACCAACATATCAAAATTTAATTAATTACTTGTTAAAAAAAACTGCTAATCATCAACCATTATTTATTGGTTGAAAAGCTTTAAATGATGTAACTAAAATTCCTAATTTTGGAAAAGATTCTAAAGAAGATTTTAGTTTCAAGAAATACCAAATTAATTTATATAATGGACTTGACCTATACCAATTAAAAAAAGCTGATGTTTATGAAATTGACCAAAATAGTCAAGAAGTTAAATTCAAAAAAACTTTTATAGATGCATTAACATACACTAACGATAAAAATTATTGAAACTCAACCGTTAATAAAGATGGTTCAGTTTATAATAATTTTATTGGCTTAATTGATCAAAATAATCAAAAAGATAAATTAGGTAAAGCTTTTGAAAAAATCGACTTGTTTGATAAATTAGTTTACGCTAATAACCCAAGTAAACAATTTAAAGGTGCACTATATGGTTATGGTAGTAGAGAATTATTAATTAAAACATTAGATGGTTTTGTGTCTTATCATGACTACCAAACACTTGCACAATTACTTTATGATCTAAATTTTAATGCAGAAGCTAATGCTGAATTTAAAACTATTTTAGATAACCAAAAACAAATTATTCCTGGCAATCAAAAAGATAGTAAAATAAAAAATATTGATTTAGCAACTAATGAAATTAAAACAATTATGATTAAAGGAATTAATAAAATCCCCCAAACTGCCTTTCAAAGATTAGATTTAGATTTAGTTTATAACAAATTAGACAAAAATGCTACACCATTAGTTAATAAAGAAGATAATGCTAATTTAAAATATATTTTATTAGTTTCACAATTTAATGCAAATGATGTGCAAAATTTAGTTATTAACAATCAATTAAATCAAAGTGCAACAGGATATTTAGGATTGTCAAAAAGCCAATTTTATAACTTATTATTTGATTTAGCATTAAATGATCAAACTTTAAAAATTGAAGCTTTTAGTCAAATTGAAAACGAACATAAAATTAAAGTTTACGATAAACGTTTAGCTAATAATAGTAATTTAAAAGGTTGAATCATAAATAATCTATAATGAATAAGCAACTAGTTCCATCCCTTTTAGCATTTGATAAAAAATATCCTTATGAACAATTAAAGATCTTTAAAAAGCATCATCTTCAAACAATACATTATGATGTTATGGATCTAACATATGTTAATAACACTGCTTTTGAAACTGAACATTTAGAATGGCTACTAAAAAATGATTTTTTAGTACACATTCATCTAATGGTTATTAACCCTTTAGAAAAAATTAAACAATATTTTATATATAAACCAACTCGCATATCTTTTCATTTTGAAATGAATGATTATCAAACTAATTTATTAATCATTAATGAAATTAAAAAACATCATGTTTTAGCAGGGATTGCAATTCATCCTTGTTTATCACTAAACGATTATGCTTCTTATTTAAAAGAAGTTGATTATATTACTTTTATGTCGGTTATTCCAGGTAAGGGTGGACAAATTTTTTTAGAAGAAAGTTTATTAAAACTAAAAAAATTATATGAATATAAAATTAAAAATAATTTCAAATTTAAGATTGAAATTGATGGTGGTATTACTTTAGAAGTTATTAAAAAAATCAATATTCCAATTGATTTTTTTGTAAGCGGTTCTTATTTAGTGAAAAATATTGAAAAACTAGAGCAAACTGTTAACGAATTTAATCAACTTGTTAATTAATATTAATTCTTAATTAATTTTAGTAACTATTTTATTTAAAAACCATTTCTTATAGTAATTGAAATGTACTAATTAATAAAAAAACACCACTGCAGGTGTTTTTTATTATTTAAAGAATTTACAAAATTAAAATTGTTTCAAATAATTATTTACTTCTTGTTCAATGATTATAATTTGTTCACTACTTAAATTAGATAAATGATCATTAATATAATATTTAAAAGTATAAATAATTCCATCATCTTTTATATAAAGATCGCTGATTTCAACTTTTCTAATATTTTTAATTTTTTTAATATTAGCAATTATATTATTTAAGTCAACGTCAGAAGCTAATTTAACACTAATATCACGTATGACATCATGATACATACTTGGCTTAATAACTTTAGTGATTACTTGTTTTTGATTTAAATAACTAGTTAAATTTAAATTAATAATAAAAATATCATTACTTAAACCATATGTTTTTTTGACTTGATTTTTTAGTTGACCAATAAAACCAAATACTTGTTTGTTTGCATTAATTAAAGCTAATTGGTTATTATCATATAATTCATTAACAACATGATTTTTATCATATGCATAATCAATATTTAATTTTGTTAATAAAGCATGTAGTAAAGCTTTTGTTGTCACAAAATTATTAACTATTAATGAATCATTAATTTTTGAATTAATTAATGGTTTACATAAAATGATTGATAAAACTGTTTGTTGACTTGAATCAACTGGATTAATCTTTGAAATCTCAAAGATATTTTCTAATTCATTTTTTTGGTTTTGGTTATATTCTAATACTTCAAGTAAGGAGTTAAGGTTATTTAATTTTAAATGCGAACGCATATTTGAAGTTGGATTATTAATTTCATAAGCTTGTTTAAAATTAAAAAAATTAAACTCATGTGCTTGTGTTTGATTTTTTAAATTATAAGTTTTACACTCAAACAAACCTTGATCAACTAAAAAATCACTAACTTTTTTTTCAAAATCATAAGCTAGATTATTTTCAAAATTTAAAATATCAAAACTGATTGGTTGTGGTTTGATTTCTTGAATACTAATTTTTTTCATAATATCTTCATAAACATCAAACTCATTTAATACATCATATCGATGACTTGGAGTAATTAAATTAGACTTATTAATTTTATAACCTAAGCGTTTTAAAAATGTTTGAGCAGTGTCTAAATCAATTTCTGCACCCACAAAATCACTTACTTGATTAATTTTATTTTTAATGACATTATGAACTGAATGTTTAAAATAATAAATTAATTTTGCATCTGATTTAAAATTAGCTAACAATTTTTTTAATGTTACTTTTGTAACATAATTAGTTAAGGGTTTGCTAAAACGCATAGCCGCTGCGGTATTGATTTTTAAACGACGCGCTGTATTTGCAATCAACATAGGATTAAAATTTGCACATTCAATATAAGCAGTTGTGCTTGTTGCATCAATTTTAGAATCATTAGAACCAATAATTCCAGCAAGTGCTAAAATTTTTTGATCATCAACGATAATTAGATCCCCTTTTTTAATTAAATATTCTTTTTGATCTAAACCCAATAAAATTGCATCTTCTTCAGCATTTTTAACAACAATTTGACCACTAATTTTTCGTGCATCAAATGCATGAAGTGGATTAGCAGTTAATAATGTTAAAAAATTCATATTATCAGCGAAATTATTAAGAACTTTAATTTGATTATTAATCAATATACTTTTAGTATTCCATTTTAAAATATAATTTTGAATATTTTTAACCTCGATTATTCCATACGAATTAACATCATCAGATAAAATCCGCACGTCAATAATTTCTTTATGTGGGCGATGATTAATCGTTGCATCAACAACATATTCGAAATCAAAATAAGCACATAATTCCTTAGCTATTCAAAAATAACCATTTAAATCATTCCGATTTGATGGAATTGATAAATCTAAAATAACATCATCTAAATTAAAAATTTGATTTGGGGTTTTATAATGGTCATAATTGTCATCTAAAACTAAAATTCCATCTAAATCTGTTGATGTTAAAAAACTAGTAGCTTGTGGATTAATTTCACTGTAAGCACATAACATTCCATTTGAAACATATCCTCTTAATTCACGTGGCGTAATTTTTAAACCATTTGCTAGTTCGGCATTTAATTTTGCTAAAACAACTCAATTATGAACACTAAAATTATTTGCACCACAAACGATCTCATAAACTTTTTGGTTTGCTTTAACTTTAACTAAATTTAAATGATTAGCATTAGGATGTCTAGTTTTTTCTAATACTTTAGCAAAAACTAAATTAGTAGATGGTTTAATGCTATTAATTGTTTCAACTTCACAACCTAAATCCATTAAAGCTGCGCATAATTCATTTAAAGGAATCTTTTTTAGTTTTGGACTAATCTTATGTAATAAATTTAAACTTAAAATCATCTTTTTTTACTCTTTCATTAAATTTAGGAATTTATTTAATTTGGGCATTAAATTTAAAATTATTTGAATATAAATAACGAATATCATCAATTTCATATTTTAACATTGCAATTCGATCAATACCCACACCAAAAGCCATCCCTGTTCTAATATCGTTAATATTCGCAGCTTTTAGAACATTTTCATGTAGCATTCCAGTTCCCATAATTTCAATTCATGTACTTTTTTTGCAAACTGCACACCCTTTTAAATCGCATTTAAAACATTGAACATCAACTTCAAACGAAGGCTCAGTAAACGGAAAAAAACTTAAACGATAACGTGTTTTTAAATTTTGACCAAATAAATATTTAATCAAACTGTCAATTAATCACTTTAAATTAGCAACAGTTAAACCTTCTTTAACTCATACAAAATCAACTTGATTGAATTGGTGGCTATGTGTTGCATCATCATCATCTTTTCGATAAACATTACCAAAAGACATAATTCTAATGTCCTTGTTTACGTTATTTTCTAAAAATTGTGCGGTTGTAGTTGTGCAATGCGTACGTAACATTTTAATTGAATTAATAAAAAAAGAATCAGCACTTGCTCTTGCTGGATGATTTTCATCAATATTTAAATGATCAAAATTATAAATAGGTGAAACAACTTCTGAACCACTTACAATTTCAAAATTTAATTTTTTAAAAAAAGATGCAATATCATCAATAATACTATTGAGAATATGATGATGCCCATTATTAATAAAAGGAGCAAACAAAGTCTTATTTGTTCATTTTTTTAAATCAACTTGATCATCTTTATCATTTAATTCTTTTAAAATTTGATCAGTAACAAATTCAATTTGTTGTTTGAAATTATTAATTAATAAACCCATTTCTTTTTTATTAGCGCTTTTTTTTAATTCATCATATAGTGGTAATAAATGTTGTTTAACAAAAATATTTTTTAATTCAATCAAATGACGTTCATTATTTGCTGTTCGTAAAATTTGTTTTAAATTTTTAATTAGATTAATAACATCCATGAACACTTACTCGCTTTCTTTTTTAGTATCTTCTTGTTGATCTTTAATCACTTTTTGATCTTCTTTATTATTTTTTTGAAAATTACGAAACCAATTCTTAACTAATTTTTTATCTTTTAATTCTGGAATATAAACGTTTGAATAAGATCAATAAGCAAAAATATTAATATTAATAATAATAATTAAAAACGAACTTATTCCAAGAATTGAACCATATTTAAATATTTTAATTTGGTTATAAGGAGAATCTAAATGAAAATTTTTGGTTTTAAAAATTTTAATAATATTTTGTTTTCATTCGTTATATTCATCAATTCGCGCATAATAATCTTTGATATAGTATGATAAACGAACAGCCGATCAGATTAAAATTGCTAATAAAATTGCTACAAAAACATATGAAATAATCGTAATAATTTTAAAACCCTCGATTGTTTTTAAAAAATCATAAAATTTTTTACCTTTTTTGTTATTCATGTTTATTTCCTAATTTATAATTTAAAAATAATTTCTTTTTATCAAAAACAAAATGTTTATGAGCATTAGTCTCATAACGTTTTTTAATCATGCTAACAATTTTAGGATTAATTTTACGATAAAGTAAAAATTGATCAAGATCGTTATATGTAAAACCAAAAAAACTCTCATCAGTTTTGTGTTCATCATCTTCATTTGCTGGAGTAGGAGCACGGTTTAAAATTTTATTTGGTAAATGATAATAAGCACCTAATTCATAAATATGTTTTTTTAGTAAACCAATTAACATATAATAATCAGCGGCACTATCGCCATATTTAGTAAAAAAACCTAAAATGTACTCATCATAGTTTAAATTACTCACAACAACACCCTTGTATGCATTCGCCATTGCATATAAATAATTTGTACGAATTTTAGGCTTTAAATTATAAAGAACATATTTATCATTTGTTTTTTTATTAATCATTTTACAATAGCTATCATAGATATCAACAAGATTGATAACTTCAATGTTAGGATAAATATTTTTTAATTCATTAATACATTCATAATCTAAACTCGAATTATGAATATCTAAAAAATAAGCATAAATATTAAGATCATACTCTTTTTTTAAATCATTTAAAATAGCTAATGTTAAAGTGCTATCAATACCACCTGAAATGCCCAAAATAACATTATTCGTTTGTGCGTCATTTAAAAATTTTATAAAATCATTTTTCAACCATTGTAGATATTTAATCAAATCATTATTCATAATTTATTTACTCTTCAAATTCTAATGTAATATCATATATTTTAATACTATCCCCCGCTACTGCTCCAGCTTGTAGTAATTGCTGGTTAAAATTGATGCTTTGAAGTTTTTGATTAAAACGAATTAAATTATCTTGCGTTTTTAAGGGAATGCGATGTGCTCAATATTTTAAATATTCACCAAAGACTTCAAAAATATGATCATCAACTTTTATAATTTCAATAGTTTTATCTAATTCTTTACTTTTAGGAATATCACTCGTTCATTTTAAAATTTGATCAACTGGTAATTGTTCTTTAAGTCGTTGCTCATAAATCTTTTTTTGTGCAAAGTAAATTTTAATAACATCATCTAACATATTATCTACATGTAAATTTGTTAACGCAGATATAATTTTAATTTCTTGATTATTTTTTAAATATTCTTTTAGAATCTGCAAATTTTCTAATGCTCCAATTTGATCTATTTTATTAGCAACCAATACAATTGGTTTGTTAACTAAATTTTGTTTATATTGTTTTAATTCATTAACAATCGTTTCATAAGCATTAATAATATCAGGATTATCATTAGGATCTAAAGAAATTAAATGAATTAATAAAAAACAACGTTCGATATGTTTTAAAAAATCATGCCCCAAGCCAACTCCTGTATGTGCTCCTTCAATTAAACCAGGAATATCTGCAAAAATGATACGATTTTGATCACGATAAATTGTACCTAAAACGGGATTTAAAGTTGTAAACATGTAATTTGCCGTTTTTGGTTTAGCATTTGTAAATGTAGAAATTAATGTTGATTTTCCTGCATTTGGTAATCCTATAATTCCAATATCAGCAATAGTTTTTAATTCTAATAAAACTTCAACATTTTCACCTAATTCTCCTAATTCATATAAATTTGGTGCTTTATTAAAAGGCGATTTAAAATGTGTATTTCCATGACCGCCTAAACCACCTTGGGCAACTAAATATTTTTGTTGATCAATATTAATATCTGCCAAAATTTCATTAGTGATAGGATTATAAACAACTGTTCCTAGTGGCACATTAATAAAAACATCTTCCGCATTCGCACCATGTTGATTTTTAATATCGCCTTTTTCACCATGTTTTGCACGAATAATTTTTTTATATTTTAAAAATTCTAATGAAGTTTCATTGTGATTACCAACAAATCAGATTGACCCACCATTTCCACCATTTCCACCAGCTGGTCCACCTTCTGGAACATGAGTTTCACGACGTCATGAAACAATTCCATCCCCACCATTACCTGCAATCAATACGATTTTACATTTATCAATAAATGCCATGCTTATTTTCCTTTTTGATCATCTTCAACTTTATATTTTATTCGTTCATTTGCTTTATTACGCTTTTTTAATTCTAAACGTTTTTTGTTAAATTCATTATAATATTGTTCTGAATCTTTAAGTTCATTGTATAGATCTGCAGCATATGTCTCAAAATGTTTTGAGCGATTCATAATAGCATTTGTAATTTTTGTCATGATAAAAATTGGTAAAATAAAAGCAACTACTAAAATTAAAAATCGTACTAAATATCCTCAAATTTGACTAGGAATAAATCCAGAAATAATTAAAATAAACATTCCAAATCAACATGAAACTACTGGCGTAAGAATGTCTCAACGAATCTTTGGCGTACTAAAAGCAAATATAATACATACAATAACTGCTAATCCTAAAGTTGAAAACATTGCAACTCAAACTGGATATGAACTAATTATATTTGTACCAAATTCAACACCATGATTGTATTGATTAGTAATTTCATTAACTAATTTTTGCATTCTTTCAATCATAAGAGGACCCGGTTTTGGTTTTAATTGATCATATACAATAGGATTTCAATGATAAAAATCTTTAACTCCTACAATCCATTGTTGGTTTTCATCTAATTTAAAACCTTTTCCATATCAACGACCATTAACACCAATTATTCCATTAAAACCTAAAAGATTGATTTCACCATATAAAATAAAAAAAACAATCATTGGAATGACAACAAAAAATGATGTAATTAAAAATTTATATAAAAGAGGTGATTTAAAACGTGTATTAGTTTCAAAGGGTTTTCTAAATCAAGTAGTTTTTGAATGCCTTAAATTCATTTACTGTCCTTTCTTTAATAAATGGTTGGTAATCAATATGATTAAATTATAACAATTTACTATATTTTAATTAATAAAAGGGTTTTTCCCATTCAATAAAACTTTTGGAGATAAAGGTTTTTTATTTGGAAGCTGAATCAATAATAGTTCAATATCAAAATCTTGCGTACTTACAAAAAGTTGATTTTTAAAAATCATGATTGTTCCAGGTTTTTGACATGACTTTTGATTAGTGATTTTAACTTGATATACTTTAATTGAAACGCCATTATATTGCATAATAGCGATTGGTTTATCATACATAGCATTAACAAAACGATTAATAAAAAAAGCTGGTTGGTCAAAATTAATAATATTTTGGTCCTTTTGAATATTATAAGCAAAACTAACAGAATTTTCATCTTGTTGAATTCCAACTAAATTTGGTTTTACTAATTCTAAAAAATGTTCTTTAATCATCAATGCACTAAGATTAGCTAACTCTAAAGTTAGTGATTTGGTTGTTGTGCAATCGTTTATTTCTAAACTTCTTTGAAATAAAATATTACCTGCATCCATTTTTTTAATCGTATGCATTAAAGTGATTCCTGTTTTTAATTCACCATTTAAAATGGCATAATGAATTGGTGCTCCGCCACGTAATTTTGGTAAAAGTGAAGCATGAATATTAACGATTTTGTATTTAGGAATATCAATTATCCCTTGATTAATAAATTGACCAAAAGCACAAGTAATGATAATATCTGGTCCTAAAATACGGATTTCTTCTTCAATTTCTTTGATTTTTTCTGGTTGAAATAACTTAATATTATGATCTAAACAAAACTGTTTAACAGGAGAGTAGATAACATTCTTTTTTCGATCAAAATGTGCATCTGGTTGACTAACAACAGCGATTAAATCAACTTCATGCATGTTAAACAATGTTTCCAAAACAATTTTAGCGATTTCTGGTGTACCAAAAAACATTACTTTATATTTCATTTAATTAAATTCCTTATTTTAATACCTTATAAATAAGGTAAAGATAGCAAAATAACTCCTTAAAATGGAGTTATTAGTTTGTTTTATTTTTTTGTTTTTCTAATTGTTTTTGACGTCGTTGAGCTGCTTTTTGTTGTTTTTTCTTATCTGCTTCTAATCTCTTGTTATAAATCTCATCATCTTTAGCTTTAATTGGTTCAATTCATAACATTTCTTCTTCATAATCTAAAGCACGCACAACATCAATTTCGCGTCGGTTTTCTTTTTTATTAATTTTTACCAAACGAACTTCACACTCAATCGCACGGGGTTCATCTATAATTAAAGTTTTAGCATTTCTTGTAGTAAACAAAACTACATATAGTTCTTTAGGTTTAGCACGTTTTTCTTTTTTAATTCGTTCCATTTCAATTTTATATTGCTCTGGATTCGTTGTTTTAAGTGCTTTGATTGCTTTTTGTTCATTTTTATAATCAATTCTTTGTTGTTTTGTCATCTGAACCAAATTGTGTGGTTCAGCACGCTTAACTACATATGAATCAATAACTTCTTTTCCTTTATCGTCAATGCTACGTAAATATTTTTTAATTGTAGTTCAAACTTCATTTGTATCTGACTTATCTTTGTTATTAACACTAGTTTGATTTTCACCATTTTGTTTACGTTTTTTACGCATAATAAATACTACAACTAATACAATTGGAATGATAATGATTAAGATCATTAAAAAGTTGCCATTACCACCCTTTGCACTATCTGCTTGTAAAATAGATTCTATTATTTCTTGGTTATCAAGCATGTAATCCCCACCTAAACATCAATTTATGAACACATTATATTATTATATGAAATTAATAGCAACATTCATAATTAATTAATAGATTAAAAATTATTTATTAGAATGTTAATAGAATTTAAATAATTTATTTAATTAGTAACAATCATACATTATAAACTTTTATATCATTTATAATTATTATTAAAACATGGTTAATAAAATAAAAACCAATTAGTTATGATTTCATTTCAAAAAATAGGAAGTAAGTAAATGTACAATATTAAATTTTTAGATTTATTAAATTCAAATTTAAAGCCTAACCCTCAATGAATTTTTAAAGATCCACATCCCATTTTACGCGAAGTAACTCAAGATATAGAAGGTAATGAATTATCAAAAGATGATATTTATTATCTTAAAAAAATGGTCCGTTATATTGATGTTTGTTATCATAATCAAGCCAAAAAATATAAAATTCGTTCAGGAATTGCGATCGCTGCAAATCAAGTTGGTTGAAACAAACGCGCAACTTATATCCATTTTAATGATGAAGCCAAAGAACATCATTATTTATTAATTAATCCTCATATTATTAAACGTTCATCTGAAATAGCTTATTTAAATCCAGGTGAAGGATGTTTATCTGTTGATGATGATCGTTCTGGATATGTTATTAGAAATAAAAAAGTTCATGTTAAAGCATATGATCTAATTAGCGAACAATTTATTGATCAAGAATTTAGTGGTATTATTGCAATTTGCATCCAACATGAAATTGGTCATTTAGATGCTGGTTTATATTATGACAATATCAATCAACAGCAACCATTTTATGCCGATCCTAGTTGAACAAAGATAGGAAGATAAAATCTTATGGCAAATAAATACGATGGGAATGCTATTAAAATCTTAGAAGGGTTGGAAGCTGTTCGTAAGCGTCCTGGAATGTATATTGGCTCTACAAGTAGCACTGGATTACATCATTTAATTTGAGAAATTGTAGATAATTCAATTGATGAAGTTATGAATGCTAACGCAAAAAACATTAATGTTATTTTGCATGAAGATAATTCAATTAGTGTTTTAGATGATGGGCGTGGTATACCAGTTGACATTAATTCTCAAACAAAAATTTCTACTGTTGAAACTGTTCTAACAGTTTTGCATGCTGGAGGTAAGTTTGATGAAAGTGCATACAAAACTGCTGGTGGTTTACATGGTGTTGGTTCATCAGTAGTTAATGCTTTAAGTTCTTGATTAATTTGTGAAGTTTATCGTGATCAAAAAATTTACCAAGCGAAATTTATTAATGGAGGTCATATTAATCAATCTTTAAAAGTAATTGGAACAACTAAAAAAACAGGAACATTAATTCATTTCTTACCCGATCCTCTAATTTTTAAAAATTTAGTTTTTAATCCTAACATAATAAAAGAGCGTCTACATGAATCAACTTTTTTAATAAAAGATTTAAAAATTATTTTTGAAGATAAAATTAATAAAAAAAAGTACGAATTTATTAATAATCAAGGTTTAATTGATTTTATTAAATTTATTAATGAAGCTAAAAAGACCTTTTCTGATGTTATTTATTTTAGAAGTAATATTAACAAAATAGATGTTGAAATTGCTTTTCAATATAGTGATCAAAATAATGAAATTATGGTTTCATTTGCCAATTCTGTGAAAACTAGTGAAGGTGGTGTACACGAAAATGCTTTTAAAAACGCTTTAACAAGTGTTGTTAATAATTATGCAAGAAAACATAATTTACTTAAAGAAAAAGACAAAAATTTAGAAGGTGATGATATACGTGAGGGTTTATCAAGTGTAATTTCACTTCGAATCCCCGAAAGCTTAATTAGCTATGAGGGTCAAACTAAAAACAAATTATTCACACCAGAAGCAAATGAAGCAGTAAAAAAAACAATTGAAGATAATTTTAGTTTTTGATTAGAAGAAAATAAAACACAAGCTTTAGATTTAGTTAATCGAGCAATTCTTGCACGTGATGCTAAATTGGCTGCAAAGCGTGCTCGTGAAGAAACTAAAAAAGTTAAAAAAATTAAAGAAGAGCGAGGAATGGGCGGAAAATTAACGCCAGCACAAAGTAAAGATCCAGTTTTAAATGAATTATTTTTGGTTGAAGGTGATTCAGCTGGTGGATCAGCTAAACTAGGACGAAATAAAAAATACCAGGCTATTTTGCCCTTAAGAGGTAAGGTTCTTAATGTTTTAAAAGCTCGTTTAGTTGATGTTTTAAAAAATGAAGAAATAGCATCAATCTTTACTTGTTTAGGAACAGGAATTGGTGCAGAATTTGATTTAAAAAAATTAAAATATCATAAAATTATTATTATGACCGATGCAGATACCGATGGTAGTCACATCCAAGTTTTATTATTAACTTTATTTTATCGTTTTATGCGACCTTTAATTGAAAATGGTAATATTTACATAGCTTTACCTCCGCTTTATAAATTAACAAATAAAAATAACAAAAAATTCTTTTATGCTTGGGATGATGTTGAATTAGATCAACTAAAAAAAGAGCAAAAAAATTATGAAATCCAACGTTATAAAGGTCTTGGCGAAATGAACGCTGATCAATTATTTGAAACAACAATGGATCCAAACAATCGTTTATTATTAAAAATTAATATTAATGACATTTTACAAGCTGAACGGCAAATCAATACTTTAATGGGTAATGACGTAAGTATTCGAAAGCAGTGAATTGATAATAATATTGATTTTAGTGTCATTGACGAATTACAAATCAATAATGAGGAGTCTAAATAAAAAATATGAGTGTAAATCATCAAAAAATTATTAATACACCTTTAGACAACATTGTTGGTGAAAGTTATGCAAAATATGCAAAATACATTATTCAAGATCGTGCTTTACCTGATATTCGTGATGGTTTAAAACCTGTTCAACGACGAATTTTATACGCAATGAGTGAATTAGGAATCTTTTATGATAAACCTTATAAAAAATCTGCACGTACAGTAGGAGAAGTAATTGGTAAATATCATCCACATGGAGATTCATCAATTTATGAAGCAATGGTGAGAATGAGTCAAGATTGAAAAAATAATCTATGTTTATTAGATATGCATGGTAATAAAGGTTCAATTGATGGTGATAATGCTGCTGCAATGCGTTATACAGAAGCTCGATTATCAAAAATTGCTAGTGTAATGTTAGCCAACTTAAAAAAAGATGTTGTTAAATTTAGTCCAAACTTTGATGATAGTGAAAAAGAGCCATCAATTTTACCATCTCTTTTTCCTAATTTGTTAATCAATGGAGCAACAGGAATTGCTTCTGGTTATGCAACAAATATTCCTCCTCATAACCCTAATGAAGTTTTTGATGCTTTAATTTATCGTATTGATCATCCAGATTGTAGTGTAGAAAAACTAATTGAAATCTGTCCTGCGCCAGATTTTCCCACAGGTGGGGAAATCCACGATTTAAATGGATGTGCTAATGCTCACAAAACTGGTGAAGGTAAATTTATAATTCGTGCTTGCATTGATTTTAAAATCAATGATAATAAGATTAATCAAATTATTATTAGTTCAATCCCTTATGAAACAAACAAGGCTTTAATCATTAAAGAAATTGAAGATATTATTTATAATAAAGAAGTTGCTGGATTAATCGAAGTTCGTGATGAATCTGATGCTAAGGGAATTAGTATAATTATTGATACTAAAAAAGATATTAATTTAGAAAATGTTAAAAACTATTTATATAAAAAAACCAACTTGGAAATCAGCTATAATACAAAATTTATTGCAATTGTTCACCGAACTCCTACACTTGTAAATCTATCTACATATTTAGATGCTCAAATTAATCATAGTTTAGATGTAATTAATAAAGTCGATTTATATGATCTAAACAAAATTTTGTTACGTATTGAAATTGTTGAAGGCTTAATTAAATGCGTTGATTTAATTGATGAAATTATTAAAATTATTCGTGCTAGTGAATCGCGTCAAGATGCAAAAAATACTTTAATTCAAGTTTTTAATTTTACAAATAATCAAGCTGAAGCGATTATTATGATGCGTTTACATAATTTGACACGTACTGATATATTTGATTTAAAAAATGAATGACAAACGCTACAAGACGAAGCTAAAACGCTACAAGAACGAATAAATTCACTTCAAGTTCGCAAAAATTATTTAAAACAAAAAATGATTGAATTTAAAAAAACTTTTGGTTTTGAACGCAAAACAAAATTATTTGATGAATTAGTAAAAGTTGAAATAAACGAAGATCAAATGATCGAAAAACAAAATTTGAATTTAGTAATAAGTCGTGATGGATACATTAAAACTGTTTCTAAAAAGTCATTTGAGTCATCTAAATATGATGAATTAGGATTAAAAACTAATGATATTCTTTTTTATCATAATATCATTAATTCTCATGATAAAATCCTAATCATTACATCAAAAGCTAAATTAATTAATTTAGTTGCTCATAAAATCACTTCTATGCGGTGAAAAGATGTTGGTGAACACTTAAATAATTATGTGAAATTTGATGCTAATGAAAAAGTTATAGCTGTTTATATATGGAACGAACAATTTAAAATTGATGAATATCAATTAGTTTTGGCTTCTAGATTAAATCTAATTAAAAGAATTGAATTAAGCGAGTTGGATATAAATAAAAATAGTAAACAAATTAGCATTATGAAATTAAATGATAATGATAATTTAATCAGTGCTAATTTAATCAAAAAAGGTCATAATCAATTTATTATTGCAATTTCTAAATTAGGATTAGCCCTGCTATTTTTAGTTCATGAAATTAATTGCTTAAATCGTTTAGCTAAAGGAATTAAAATTATGAAATTAAAACCAAATGATGAGGTTAGTGCTATTTTAATTACTCCTAATAATGGTTATAATGTTCAACTTTTTTTAGAACGGGGAAGTAAGTGTTTTAATATTAGTGAATTAAAATTATCAAAACGTGCGGCCACACCAACAAATTTATATCCAATAACAAAAAAAGTGCAAAATGTTTTAGCAGCTTTTTTAGTTGCTCATGAAAATGTTTTTTATCTTTTAGATCAACAACAAAAAATAAATCCATATTACTTATCAAACCCCAAACCAACAAAACTAGATACTAAAATTAGTATCTATGAAAACGATCAAATGATCACTGATGTAGTTAAAGATTGCTTTTTAAGTGATGAAGCAATTAGTGATTTTAAAAAAATTAATATGTACGCAAATGAATTTGATAATGAAATGCTAAAAATTAATAAAAATCACGATGATTCACAATTAGAATTAATTGATGAAGAGGAAGAAAAAAAATAATGATAACAAATAAAAAATGTAAAGGCTGTGGAGCTTTTTTGAATGATCAAATCAATACAATTGGATATACCCCAAAAATAAATGAAACAAAAACCAATTTATGCCAACGTTGTTTTAAATTAATACACTATAATAAACTTGAAAAAGTAAGTACTAATTTAAATCAAAGTATTAAAGAGACAATTAACACTTTAAATTTTAGTAATTTACAGATTTTTGCTGTATTAGATATTTTAGATTTAGAACACACAATTATTGATGAACTAAAAGAATATCAAAATAAAATTATTTTTTTAGTTAATAAAATTGATTTATTACCTCAGCGCTACAATAGTGAATTAGTTAATGAAAATGTTGTTAAAACACTTATTAATCATGGATTTATTGATCCTAAAATTATCTATGTTTCCACACATTCTAATACCAGTTTAAAAAAAGTTATGGATTATATTAAAAATGCAACTAATAAAAAACAAAAATCCATTTTTTTTGGAAAATCAAATGTTGGTAAATCAAGTTTAATCAATGCTTTATTAGCGCTAAATAAAATTAAAACTAAACTTACAATTAGTAGTTATACTAATACAACAATTAATTTAAATAAAATTAATATACAAGAACATCAAATACTTGATGCTCCCGGAGTTTGTTTTGATGAAAACATTTTAAATTATGTACGTGATGAAGATAATAAATCAATCATGATTTCTTATGGTGCTAAAGCAATTAATTACCAAATTAATCCCCAACAAGCTCTTATGATTTCTGGATTGGTTGGAATTCAGTATTTAAAGGGTCAAAAAACAACTTTTACATTATATGTTTCATCCCAATTAGATATTCATCGTTGTAAATTAGAAAATTTTATAATGAATTTTAATAAACGATATTTATTAGCGAAGTTCAATTATCTAAATCAAGATCTTGAATTTGTTGACCATCTAATAACTTTAAATAAGGATCAAAAAACCAATATTTGTATTGCAGGCCTAGGTTTGTTAGTTATTAATGCTCATGCTGAACAAATATGTATCCGTTTACCAAAAAGTGTTGGTCTAAAAGTAGCAAAATATGCGATTATTTAATTATTATTAATAAAGAGAGATTTGTTATGAAAATCATTTTGTTTTGTGGAGCTTTTGATATGGTTCACAATGCTCATATTGCAATGGCTAAACAAGCCATGAAGTTAGTTAATGCTGACAAACTAATTTTTTTACCATCAAATTTTAAATTTTTTAAAGCAATTAATAAAAATGACAATTTAGAGTATGAAAAAACAAAATTAACACCTGGACATCATCGTATAGCCATGTTAAAAATTGCAACCAAAAATTTAGTAAATATTGAAGTAAGTGACTACGAATTAAAACAAATTAATAAATCTTATACAATCAATACCATTGAGCATTTTAAACAAATATATGGTAGTGAACATGAATATTATTTTATTATGGGTAGTGATAACTTAGAACGTTTTAAACAATGAAAAGATTGGGAACGAATTCTAAAAGAAGTTAAAATTATTTGTTTTAAACGTGGGGATGTTTGTGTTAAAAAAAGTTGCCCACAAAAAAGCTGTGAATGCGAAAGTTTTAATTTTTTCGAACATGAAATTCTTTTGGTTAATGATTTTAATTACAATATCAGTTCTACTGAAATTAAAAAACGCCATAATTTAACATCAGGAATAGATCCAGCAGTCCTTGATTATATTAATGAACATGGTTTATATGCTTTATGATTATTAGAAAAACATTTAATTTCATATGATAATTTTAATAATTTAGAAAAAAAAGTTGCACGCATCAATCATTGTCGTCGCGTTGCTCAAATGTGTGTTGATTTAATGAATGTCTATGATAAAAAACTAATTGATCAAGCTTATTGTGCGGGAATTTATCATGACATACTAAAATGTTTAGATGAACAAGAAAGTGTTGCATATTTTAATGAACATAAAAGTGAATTAAATATTGGTGATGATTTTATCTCTTGAAGAATCTTACATTCATATCTTGGTGCACATTTATTACAAACCCAATATGGTTTTAAAAATCAATTAATTTTAAATGCTATTCGTCGTCATACAAGACCTTTTGATTTTATTAAAGATTATAGTGAATTAACTACTTTAGATAAAATTCTATATTGTGCTGATAAATTAGAGCCTAACCGTAGGGAAGAAATTGATCAAATTAACATTGATTATTATCGAAAATTAGTTTTTGAAGATCTAGATAAGGCATTTATTGAAGTTTATCAATACCAACAAAGACAAAGAAAATAAGGTAAAAATATGATTGGTTTAATTGTGGCATTAAATAGTGAAATTAAAACCTTTTTTAAACAAATTAAAAAGAAGGTTTATCAAATTAATAATATTGATTTTTACTTGTGCACTCATCAAAATATAGAATTTGTTTTAGTTTTTACGGATGTAGGAAAAACTAATGCGAGTTTTATTACTGCATTATTAATTAATAATTTTAAACCTAAAGTGATTTTAAACGTTGGTAGTTGTGGTGCACTAAATGATCAGTTACAGGTTTTAGATATTGCCATTATTGATCAATGCCAATACTTAGATGTTAACGTTAGCGCTTTTGGTTATTTAAAAAATCAAATACCGCGTTTAGATAATTTTTTATTTTAGATAAAAGTTATAATCAACAAATCAAAAACCAGTTAATTAAGAAAAAACTAAAATGTTGAATTGCAAATGTTGGAAGCAGTGACACATTTATTAATCGTGATAACATCTCCTTTTTTTATGATCAACAAATCGACTTAGTTGATATGGAATTAGCAGCTATTGCTCATGTATGCACGCGTATGTTAACACCACTAGTAAGTATTAAATTAGTTAGTGATCATATTACTCTTCCCAATTCCAATCAAGAACAGTTTAATAAAAACTTATCACTAATTGATAAATGATTTAATGAGCATTTACCATCCATTATTGAAGCAATTTTAGAGATCTACTAATTTATTTTTGTAAAAAATATTAAAATTACAAAATCTACTATTATTTTTTAAATTGTTTTATAATTTACGTATATAAAAAGTTAGTTAAATAAGGAGTAGTTTATTTATGATTTATAAAGAAATTGTTCGTAATATTATCAAGCATCCATTAATCTTACCAGAAGGAAAACATTACCATTTACATAAAATTCTAACTTCAAAAGATGAATTACGTAAGGGTGAAGTTAGTTTAGTTGCAGAAAATGGCAAAGAATACACTGTTGATTTATCTGCATTTGTTGACTTATTAGTTGATGGTGATTGCATTATTGATGATAATCATTCACTTGTTGCTTTATACTTTGATGAAAGTAAATAAAAAGTAAAAAATATTAATTTAAGAAATCTTGCTAAAACACACCAGTAGGTTTTAAGCAAGATTTTTATTTGTAAAAATTAAGTATTTATTATGTTATTTATTTTATGAAATTTAATAATAATTTCAATTATTATGGGTTTTATTTTGTGTAAATTTTTAGTTTTTATGTTAAAATTTCTGTGTTATTTTATTAAAACATTATTGTTTTTTTTTTTTTTTTGAAAAAATAATGAGTAAATAAATGTTTTGTTTTTATCTGTTTATTTTATAAAAATGTGGGAAAAATTATATGAAATCAAATAATAAAAATTACAAATTTTTGAACATAAAAAAACGGAAATATCGCATTTTTTTTATGTTGTGTTCTTTAAATTTAATATGAGCAGGCGTTATTATAGCTATTGTTCTGCGTTTAAAAAATATTGATAAACGCTTTAGTGAAAATGACCATAAAATTGCTCGTCCAACTTGAGAGTATGATAATGATGGAAATTTAGAGATTCACACTCAATTAGCAAATGATTTAAATGATGATTTAAAACAAAAAGCTTTAAATAATGCAAATGTCAAAGGAATTGTTGTTGACCAAAATGGTGTAGAACATGAGATAGATGTAAGTATTGATGCTAATGGTAAGGTTATTATTCCAACAAAAGATTTAGTAAATAATGACCCAACTAAACCTAATATATACACACTTAAAAAAGTCGTTTTAAAACAACATAATCAACCAAATATTGATCTAATTAGTGAAGAGCAATTAAGTGGTGATAATCATATTTCATTTAAAAAACCAACAGTGGTTGCTCAAATTAATGATAACAATGATTATATAATGCATTTTACGAATCCTAATTTAGTTAACAAGAAAATTAAATTAACATTTAAAGTTGATGATCAAGATGATCATACAAAAACCATTGAAGCTATCATTGGATTAGATGGAAAGGCTATTTTTAAAACAAGTGATGATGCAATTTTTGCACCAGATCATAAATATACACTAACAAAAATTGAAGCTAATAACAAAAAAGTTGCTAACATTGATGAAATTCCATTAAAAAATAGAATCGTTGATAAAAATCCAATTAAAAGAATTAACTTAACACAACTAAAAGTTGCTAAAGATTTAATTCTTTGAAATAATACAAACCAAAACCAATTCATAACTGCTACTTTTAAAAATATAAATAACGATTTTGATGATTATATTGTTAAATTAATTTATGAATACAATTATCGTGGAGAAATGAAAGAAATTGCATCACAACCTTTCAAATTAGAAAAAAATAAAACAACATACAACAATATCCTTTTGCCAATTAGTATTCCAAATCGCTTATATCATTTTAAAAAAGTAGTAATTCAAAAATTAAATCAACCAAATAAATCTATTGATTTAGATCATGATCCAAATTTAAAATCTTTTTTTATTGTTGGTCCTGGTAAAACAGATTTTGCATGAAAAAAACCAAATGATGATCAAATTACATATGATGGTTTAAAGAGATTAAATATTGAAATTACTTCTCAAGATTATGCATTACAAAATGGTAAAAAAGTTATGGTTTGGTTTAAATCAGATGCCGAACCAAATAATGATTCACTTAAATTTATAGGCGAATTATCTCAAACTAACGCCAATGGAACAGCTGCTGTCATTAATAATTTAAATAATTTATACGGTATGAAAGAACAAACAGAGTATTACATCTATAAAATTCAGTTCATAGATGAATTAGAATATTCAAAATTACCACCAAACCGTAATAACATTGTTTATGAATATAATAAAAATATTGCACGTAATTATGCATTCCGAACTAAAACTGCCCCTTTAATTTTAGAAAAAATAGAAAAAGATGATAATGGTAGCATTGGAGGACAAAATTTTTATTTTTACCTAAATAAAGCTAGTGATGATCCTAGTACAATCAAGCAAGTTACTATATATTGTCGTGATAATAATAATGAATTATATACTTCTGATGATCAAGAAATAGATTCTGTTGATCTTGTATATCATTCTTGAATTCATGATTTAAAATGAAATCGTGAATATGTTTTTGATCATGCTGAAATACAAACAACATATCCTGATGATCCTGAATATGGTGGTGAAACAAATGATTTAGAAATTAAAAGAGGATTAACTTTCAAGTTTTATACACCACCAAGTAAAACAGAAATACAGTGAAAACATCCTATAAAAATAACTAATAATGGGGCAATTTTAGATATTCAATTACAATCAAAAGATCGGGCATTTGAAGATAAGCAAAAATATCGAGTGACATTAGTTGAAGCTGAAAATCAAAACAATGCTGTTGTTGGTGACTTTTTACTTGCTGGCACTTCTATTAATAAAAATGATAGCCCAATTGGTGATAATAATGATAAGAACAAAGATATAGCAACTTTGAGCTGTAATTTTGAAAACAAATTAAAAAAAGGGACTAAATACTTATTAAAAGAAGTTCGATTTATTGCTAATCCAAATGAAGAAATTAGTGCTAAACCTACTAGAGCTGCTAGACCATTTAATAATGATAATATTATTTATAGTTTGAATCTACGAATAAATGATCCCTATGACTTTACAACTTTAAAAAATTAGTTTAATCCTTAAATTTTTAGTTATATATATATATATATATATATGAAAGTAAATAAAATAATTATGAAAAAAAGAGTTAATAAAATATATAAACACAATATGTTTATTTTGCTAGCTACTTTAAATTTAGTGTGAGCAGGCGTTATCACAGGAATTGTTTTAAGACTAAAAAGTAAAGATTACTCAAAGCATACTATCTTAAAAAAATATTATTCATTTGATAAAAACGGCAATTTAATTCTTAAAGGTTATTTAAAACCACATGATAATACTAATTATGTTTTTGGGATTTTTAGCGATGAAAGCAATCAAGAACATAAAATTCAAGCACTTGTTAATGATAAAAAAGGATTTGAATTTAACACTAAATCATTACCACTAAACCATAGTTACAAACTTAAAAAAGTTACAAGTATTAATGACTATAACCATGTTTTATTAACAAATGATGATTTAGTTAGTCAGCAAAAAATTTGTTTTAGTAAGCCAGTTGATGCAAAAGTTAACTTTATTAATAATAAAAAAGTTTATCAAGTTAAACTAAACACGTTATTAAAAAACACTTTAATTCAATTAACACTAAAAGACTTAGAGCATAACGTATATAAAATTACAGCCAAATCAAATGATATGGGAGAAGTTAGTTTTGATGTTTCACAATTAAAAAATAATAATTTATATGAGGTAATTGCTTTACAAACACAAGACCAAACTAAAATCGTTAATGTTAATGAGATTGAATACAACAATAAGATCATAAATAATTTAAATATTGATGAATTAAATACACCATACCAATATAGCAAAAATGGTCATCTTAACTTAATTGCTAAGATACCATTACGCTATGCTAATGAACAAGTCTATGGTGTTTTTGAAGACCAAAATAACCAAAAACATTTAATTCCTGCCGTAAATAAAAATGATGGAACAATAATGTTTGATACTCAAGTTTTAACTAAAAATCCCAATAAAAAATATCATTTACAAAAAGTTGTTTTAGCAAAAAATCAAAATCAAATTTTAATACATAATTTTGATTTAGTAAGTAATCAAAAACAAGATATCTTCTTAAAAGAATATAAAGTTATTAGTATTAGTGCTCTAGAAAATGATGCTGATAAAACTAAAAGAGAAATCAATTTAAAACTATCTAACATAAAAAATGACTGAGTTAATAAGCAAATTAAAGTTATTTATTCAAGTTCAGATGGTTCAGATGTTCAAACTCAACCTTTAGTTTTAGAAAAAAATCAAACAAACTATGTATTAAAACTAAATAATTTAAAAGCAAATCGTTTATATACACTAAAGGCGGTTGAATTAATAAATAACAATGGTAAAACTAATTTACCATTAGATACTAACTTAACTAATAGTTTTATGGTTGAAAGAACTAGTGCTGTATTAGCAACAAGTATTAGTGAAATAAGTAATCGTTTGGGAACTGCTTTAACGAATGCACAAGTTAAAATCACTTTAAAAGATGTGGATAATGTTTTATTAGCTAATCAAAAAGCGATTATTAACTATGGTAATAATCAAAAGAGTGAAGCAACTGTTATTGTTAACAATGGTGTTAAGTATCTAATAGCAACCTTAACTAAATTAACATTAAATACGCCTACAATAATTAAATCCATTACATTTGAACAAAAACCAACATGTGCTATTGAAAATATTGGGTTAGATAAAACAAATAACATTATTTATCAAAATACACATGAAGCAGCAATTCCTTTAGTAATTAATAATAATTTTGAAATCAATGGGCCATTAGCATCAAGTCATGAAAGTTTAAAAAGTATTAATGCATCTGATGCTAAAAAGGTAAATGCAATTAATTTAACCCTTGACTTTAATACCAATGAACATATTTATAAAAATTTATTTTTTAAACTAAAATATGTCCCTATTGACGATCAAGGTAATGAAAATATTTATGACGCATCAGAAGCAATTTATAGTGATGTTTTAAGTGCAAATAATATAATTGCTAATAAAATCCAATTTAGTTTAGGTAATTTACAAACTAATCGTAAATATAAATTAAAAACAATTTATTATTTAACATCAAAAACAACACCTTTAGATGATCATAACGTTGTAATGATCAAACCAAATGTTAAATCCGAAATTATTGTTGCCCCTAATGATAGTACGATTGTAAAATATGGTAATTGAAATCAAGATTCAATTACTAATGGATCAAAGGCTATTTTTAAAATTAATTGTAATGATGGTGATATTTTAAGTGAGGATTTAAGTGCTACACTTGTTTATGAATCTAATGATCCTCATGATTTACAATCATACACTACAAAACTAAAAAAAGTTAACAATGATTGGGTGATTGATTTTAATTTAAATAATTTAAAACCACAAACCACTTACCATTTAAAATCAATAACAATAGCAAAACCAAATAAAGCATATACTAATTTAAAAATTCAATCACTTCCACAAGTGGATATTAAAACGCAATTACCTAATTTAATCTTGCATGATTTAAAAGCCACAAGTGCGGTTTTAAAATGGGATTCTACTAACAATGGTAAAAACAATCACCAAACAATTCAAGCAACTTTTTCTCATGTAAGTGCTAATTATGATAATCTTAATGTTAAATTGGTTTATGAATATAATTATCGGGGTGATATCAAAGCAGTTGAATCTGATTTAATTACTCTTAAAAAAGACCAAAATGTTTATCAAATAAATTTACCAATCAGCGTTCCTAACCGAAAATATACTTTTAAGAAAGTTTTAATTCAAGAACGCAATAATTTAAACAACTATGTGGATTTAAATAAAAAGATAAACTTAACAGATTCATTTGTAGTTAGCCCTGGAAAAACAACATTTGAATGAACACAACCAACTAGTGATCAAATTAGTCAAACAACCCTAAAAGCATTAAAAATTAAAATTCTTTCAGAAGATAAAACGCTTGATAATAATGTTAAGGTTATTGTGTGATTTAAATCCCATACAGATTCAAATGATGCACTTAAATGAACACGGGGAACTTGGGAGGTTAATAGTGATGGATCAGAAGCGATTATTAATCAATTAAATAACTTAAGTGGATTTAAAGCAGGAACGCAATACTATCTTTATAAAATTGCATTTGTTGATAATCTACAATATGGAAATACGCAAGCGAATGATAATAAAGTAATTTATGAATGAAAAGCCAATGATACTAAAGAATATGAATTTACAATGAAAAATGCACCAACAATTTTACAATCAATAAAATTTGAAAATTGTGATGCTAATGGTGTAATTAATAATGAAGCGGAAGGAATTGCAGAATTTTCAATGACAACAAACAAGGTTAATGATGATTTTGCTAACCGAAAAGTTAAATTCATATATCATGACAATAACAATGTTGCTTATGAAACTGATGGTTTTCCATTAAGCAGATCAGAAACAAGTCTTCATTTTTCTTTATCAAACATCCCTAACAACCGCGACTATACTTTTGACCATGCTGAAATTGAAACTAGTCCTAATAGTAATAAATATGAAATTTGACATTCTCAAAACAAGATAATATCTAAGTTCAAACTTAAACCAGCTCAAACTTCTGTAAAATATATTGATAAACAAGAAAGAAATAATAATAATGTAGATTTTGAGCTTCATTTAGGTTCAAGAGACTGTGCATTTGAAAATAACCAAAAATATCGTGTTACACTAGTGTCAGTAGACGCACTCCAAACCGAAATTTCTAAGGAATTTTTACTTTCTGATGTTAAATTAGATTATCAAGATGGTAATATTGGAATATTAAAATGCCATTTTGATAACTTAATACCAAACACCGGATATACATTAAAAGAAATTAAATTTCTTGCTAACCCAAATGAACAACAAAGTGCAAAACCACATAAGGTAATTAAACCTTTTAATGATGATAACAATGTTATTATCGATAAAAATTATTCTTGAAAATGATATATTTATCCTTCATAATCATTAATTTTTATTAATATAGTAAGATATCAAAACTCCTCTACTAGAAAAAGTTCTTTAGAGGAGTTTTGATATTATTTAGCATTTTAATGCTGTGGTTTTTTAATTTCTTCAATCAGTTTTGTTAATCTTTGAATATAAAGATTAAGAGTATAAGAAGAAACATTTTCATTACTTATTCCTTGTTCAACAAAACTAGTGAATTTTTCTAATCGTTGTAATTTTGCTTTATTATTTGTATGTTCATTTTTTGTTTGAATAATTTTTTTGCTTTCATCAATTAATTCTTTAGCTTTGGGAATATAAAGTTTTTTATCAATGATAGTAAAAACTCTAACACTAAGATTTTCAAGTTCTTTGCTAAATTCTCTTTCTTGATTATTATTTAATTCTAAATAAGCGATTAATTCTTGTGGTTTTTGCAGCGAATCAGAAGCATATGTTAAATCAATTTTTTCAAGATTATTTACTGTTAATACTTCGTTTGGAGCAAGTTTTAAATTAATTTTAGTATTTAAAAATAAATCCTTTTCATAACTTTTTAAGACTTCTTGAATGGTTTGAATTCGATTAGCTTTTTTTATTGGATCGTTTTCATTATTTAATTCAATATTAAACTCTTTTAAATGTTTAATGAAGTACTTAAATAAATAAATTTGTTTAAAATTTGATAAAATGGTTTGTTTAATTTTAATCACAACATCATTAATTTGTGAATTAGCTTTTTGAAATTTTTTAATTAATTCATCATTAAGATTATAGATTGCATCAATTGATAAATTAGTAATGTTAAAATCAAATAAGTTAATGATTGATTTTAAACTTTTAATTTGGTGTTCTAAACCATTATATGTTGCTTGAGAATTGTTTTCATCTTTTTGCTTATTTTCTTGTTTTTCAATACTATAGTAAGGATTATAATCATCTGGTTGTAATGATTCAATAATACTGATTAATTTGTTAACACTATTTTCACTTTCTAACGCAATCATTTTTAAACCTTCTAAGTAATTTTGAAGGTCAACCAAAAGTTGATTAGTTAAAGTTTTAATTTCTTTGATCTCTAATGTCTTTTTAACATTTAATACATGATTTATATTCTTTATTAAAATTTGTCTTAAATTTGTTAATTTAGTTTGATCAAATCATAGAAAATGATGATTTTGTTCATAAATAGTATTAATAACATTAACTATATCATCTAATTTTTTAATAAAAGGTAAAGCTTTTAGTTCTTTGTTAATTAAGTCATTAATATCATTTAATTTAGTGTAAATTAATTTAAAATCTTCATATAGTTTTTTAAAATTAGCTTTTGTGTCATTATTAACTAATTTTTGAATATCATTTTCAAGCTCAACAATGTTTTTATTTAATCCAGATGTTTTAATTAGGCTTTTAATTTTATTAGCTAAATCTAAAATTGTTTTTTGAATTAAAGCTTTGGTGTCTAATCTTTTTTGATGAGCATCAGATTTTAGTTTATCAATTATTGTAGTATCAACACTTGCTTGATTAAGTAATTTTTCATATTGATTTAAAAAATCTTGTAATTGTTTTTTAGTGTTTTCTAATTCAACAACATCTATTAAATCACTCATGTTTTTGATTGAATCTAATTCATTTAAAACACTAACTTGTGTAATTAAATTTTTAATTGTTTGTTGTTCTTGTTTTAATTTTTGCTCTGATAATTTATTAATGTTTTCTAAATTTGATTTAAGAGTTTGATTATATATCTTTTTAAAATCTTCATAAGATGATTGATCAACTATTTCTTTTAATAAAACAGCTTTATTTAAACTTGATTGTAATTCTTCTTTAGCTTTTTGTTTTGTATCAACTTTTGCACAAGTTGTAATTATTGTTGCTAACAAAGGTATAGCACTAATCATACTTAGTTTCAATCAAATTGATTTGTATTTTTTGTGTTTCATATTTAAAACTCCAATAATTAATTATTCAATTACTAGCTTAACTTCTTAGTTATTTAAGTATTATTCAAATTAAACGTTTAAATATTTTAACATTATTAAAATAAAAATCTACTCTAGTTTTATCCTAAAGTAGATTGATCTAAAGTTAGATGTTCTTAAAGTAAGAAATCTAATAAATGTTTAATGTCAATAATAAATTTTGCTTTATTCGCAACTAGTTGTTCTGGAGTTTTGTTAATACTTGTCTTTTTTAAAAGATCAAAATTAACTTTTTTATTTAAAACATCATTTGAACTAGCTCCATAAAAATAAAAATCTTTTTGATTTTTCATATTAATATAACAAACTAAAATCGTAATTTCTGCTTTGTCTAATTCATCTAATATTAAATTATCGTTATTGTTTTTATTTTCAATATATCGTTGATATGATTCAATTAATAATTTTGTTTTTTCATAATCATAATCACTATATAAATTTTTTACTTCAATATATAAATAATGATCTTTTTGATCATGTGTTTTTATTTTTAAAATAAAATCAGGGTATGAATTACTTATTTGACCATCTTCATTAAAATATTGATATTTTAATCCATGAAAAACAGGATTTTTAGTCCAAAGTTCAACATTATTTTTAATTTTATCATTATCATTTGATAATGAAAGGAAACGATTTGCAAATTTAGATTCAGTTAAAGAATCAAAATAGTGATTTTGTTCTTTAAGATCTTTATATGCATACTTATCACAAACATTTAAATCTAACATGTTTTCACATTTTTTATATTTTTCAATGGGAATAAAAATTTCATTTTGATTAGGTAAATTTTTATTATTTTGCAATTTAAAAAGTAATTCATGATTATCTTTTTTCATTTGTTCTAATGAATTTTTATATATTTTTTCAATTTGATCAAGATATTCTTTAATAATAACATATCAATACATGTTGATGCTTATGTTTAATTTTCATCTTTTTTCTCACTCATTAAACTTCGTTTCAAGAAAATCAATAATTTTTTGAGGAAATAGATGTTTGTATTTTCTTAATTGTTCAATAATATAAAGTTCAAGTTCGATTGAATTTTTAATTTTAGTATAAACACGTGTTTTACCTTCAATTTTTGAAGTTTCGCCTTCTAAGTATTGATTTTTTACAAAATTTTCACGATATTCCTCATTAACATAGTATTCAAAATTACTAATTGAATCTTCAAACTTCTCTACTATTTCTTCTTTATAAGTTTCTCCATTTAAATAGTTATTAATTTTCTCTTGATTAATTTCGCCATAAAAAAATCTATCATTTTTATATTTGTCTTTTAAAATCAAAGTTTGACGAATTTTATCTTCTGAAATAATATTAGAATAAATAAAATATTGATTTGATATGGAATTATAGCTAATTTCGTTTTTGTCAAAGCTAGGATTTGGAAAACGCTTAATTCTTCCAATTGTTTGTACTGATAAATTTTTTGAAGAAACGTTTCGCAATTGTACTAACATGCAAGCACGCGGAATATTTCAACCAGTTGCAGGACCAATTTTAAACAAAATTACATCCACATTTGAAGTCTTTTTAGAAATTTCTTTTAGAGAAATTTTATTATTTTTTTTATTAATTAAAATATTAGTTTGAACCTCTTTATCATTAGAAAAGTATTTAACTCATGATAAATTATGTTTTTCTAAAATTTTAATAATTTTTTCAATTTTATCTAAAAATTCGCTTTTTTGTGGTTCTTTATTAGGTTCATTTTCAACTTGAATTAACATGGCTGGACGAATATTTTTTAACTCTTTTGCTTTTGAATATTGCTCTTTGATTTTTAAAAATTGCTCACAAGCAGTTGTTAATAATAGTTCATCATCAATGTTTTCTTCTTTTAGTTCTTTAATTTCTAGATTATAAACATGTTCATCTTTTAACAATTTTGTATCATCATCCATTAAATCTTTTTCATCAATGAATACTAATTTATGAATTCCTTTAGGAGTTGCTGTCATTTTAATAATAAAAGATGCTGCTTCTTGAATTTTATATTCAAATCGTGTTTCTTCTTTTTTAAAAGAATTTTTGTGTATTTTTTCATCAAAATCTTCAAATATTTTTGTTTTATTAACATTACCGCCATGATGAGCTTCATCACGGATATAAACTAATGTATATCCTTGTGATTTAATTTCATCTAAAAAAGCATCTAAAATTCCTTCTTCTGTAATAATTCTTCCTTTACCAAATGAAGAAGACCCAAAAATAATTACTTTATTTTCTTCAGCTAAAATATTGTAAGAACTATCTTTTTTAGCACTTTTAGAAGATGGTGATTCTTTAAGTTCGACATCAAATAAACCATTAATATAATATTTATACTCATTCATATTTTGTTCTAATTGAAATGGTAAATCAGCTGATGATAAAGTCGCAATTACAAAAATTAATTTTTGAGGACTACCATTAGCTTTGTTTGTCTTAATAATTTCATTAATAGTATTGGTAATAATAAAGGTTTTACCAGATCCTGTTGGTGCTTGAAAATCAATAATTTCTTTTTTATTGTTATTCACGCTTTCTAAATAACAATTAACTAATTGACTAACAGCTTTTTGTTGTGATTTGGTTAATTTCATAAATTTTATTCCTTTTTTTCAATTGGTTTTAGTGTACTTAAATTAATTAATAATTCGCGTTGGTTATTTTCAAATAAACTTGATTGACTTGTATTATTAAAATCAACTAACATTTTAGCAAATACTTTTTTAATCTTGCTATTATCAACTTCAATTGGATTAGTGTCAAAATATTTTAAATCATAAACATTTAGTTTATTTAAATATGGTTTATTTTTACTAACTCAATCAAAATCGATTTCGTTTTTAGTTCCAATACCATTGTTGATACGATATAAGCGTTCATAACAAACATTAGTGCCTATTTGATTTTCATTGTTTGTTATTAATGTGAATGTTCGATTTCCACCATCTTCTTTATTTAATTCTAATACTGCATGACCAGTTGTTCCAGATCCTGCGAAGAAATCTAAAACACGAGCATTTTTATTGGAATGTAAATCTAATAAATGCATTATCAATTGAATTGGTTTTGGGTTTTGAAAATCTATATTTAATGAATCAACTTCATTATTTCCATCTTTAGTTCAAATACCTGACACAAAAGTAAATGGAAGTTTAATTATGCTATAAGTAGTTTTTCTTTCATCATTAAATATTTTTGCTACTAAATGCTTTCCTGGCTCTTTTTTAAATGGAACTAAAATATAATTTTTATTATCTAATTTTAAATTTCATTTTTCTTTATCTTCTTTAGATAAATCTTCATAAGCTTTGTTTCTATGTTCGAATTTATTATCTTTTTTTCCAAAATTATTTCTAATAATATCGCTATTTATAAAAAAAGAATTATTGTTTTTATCTTTATATTTAATAAAACCACTCATTTTTTTATTTTTTTCAGAAAGACAAAAATTGTAATTATTTTTTGAATAACATAATATATACTCATGGCCTTCATATATTAATTTATTCAAACCACCACCAGATTTCTTTTGCCAAATAAAATTTGTAACAAAATTCTCTTCGCCAAAAATTTCATCCATCAAAACTTTTAAGTATGCTTGTTCATTATCGTCAATGGATACAAAAATTACACCATCATTTTTAAGTAAGTTTTTAGCTAATTTTAAACGTTCGTTCATCATATTTAATCAACCATTGCGACTAAATTTATCACGATAAACAAACTTAGATGCAACTACATCATCTTTTTCGCTAAAATTATTACCATCAGTCTTGCTTGCTTCAGTATTATAAGGAGGATCAATATAGATTACATCATAGTAAGCGTCGCGCCCACTAGTCTCTCTCTCTCTCTCTCTCTAACACTAATAAATTCTTTAGTACATCATAGTTTTCACCAATGATTAAACTATTAGTATCATTTTTAAATAAATTATGTGCATCATTCATGAAAGATAATTGCTCATTTTTAGCTAATATCGCAATTTGAGTTTGGTGCACGCTTGGAGCAACATCAAAACTAAATCCTATTTTAACACGTTGAATTAATAATTGAAACACATTATCTAAATCCTGGGGATCAGTTTTTTCTAAAATTTTTTTAATTAATTGTTTTTGATCCTCATTTAATGAACTCTTACTCATTTCATCAATGTCTTTTAAATAACTATTTAAATCTTTAATCATAAATCCTCTTTATAAATTAAATATATAATCTAATTTATATTATAAATGATGCTGAGATAAACTTAAATATCATTATTAACCAAAATTTAGTTATTTTAATAATTAAAAAAACTTTTAGTAAATTTATAATCACTAAAAGTTTTTGAATTTATATTAATTAGTTGTTGTAAATGAATAATCATTGTGTTGAGGATTATCTTTTTTTGCTTGATTACGGTTATAAATTTCGTTATTGTTATTAGCAAAATTTCATTTATAAGTTAAATAATTTGGCTTTTCACTAAAGTAAATACGATTGATATAGTATTTTGTATTCGGTTCTAAATTATTAATTTTATTTTCTGATATTAATCATGATGTTAAACCTGTTTGTAATTCTTGATGTTTATTAGGGTCAATATGATTTTTAATTAATCCATCAAATTCTTTATCTGTTTTCGAAAGTGTGATTTGATAAGGTCCTTTAATATTTCCTTTATCATCAATTAATTCTAAATTAGCAACTAAACCATCATGAAAAGCTTGGTCGTGGGAAATTAATTCTAAATTAAGATTAACTTGGGTTGGTGAAATGTTTTTAATATACTTATTTTCTTTATTTTCATTAAAATCACTTGCAGTTGTTAAAATCGTGTTTTGACTTGGACGACAACGAATTGTTATAGAGCTTTGATTAAAAATTGTTCAAGTATTATCATCGTTTAAAACTTCTATTCGTTCAAAATTATATTCTCGATTAGCAATTGGTAATGGAATTTTAACATACATACGGTTTTTTAATTTAAATAAAAGATTTTCTCTATTAATTATTTGATCACTACTACCAATATCATTGCTATAAGCCATAAACTCTTTTTTTGTGCCATCAACTGTTGTATAAGAAAAAACAAAACGTGCTTTTTTATTTACTAATCAAGGATATTTAACATTTAAAATAAATAATTTCACTTCACAAACTGGATGATTAGTTTTTTTATCAACATCAAGTGTGTTTTTTAGATATTTTTCCACCAAAGCTATTGATAAAGGATTTTGTAATTCAAAATTATAAGTAATTTGATTTAATTTATGTTTTTCATCTAAATTATTTGGATTATTTTTATCTAAATAATAAATATTATCTAGTTCATATAATGTAGCAGTTTTAATGTCATCAAACTTTAAATTAAATTCGATCATTCACTTATTTATTTCATTTTTAATTGGTGTACAACTAATAGCGTTAGTTTTAATTTTTTTGACAATCTTGTTGTCTTTGTATTTAAAAGTTGCAGCAAATGTAAGGTTTTTTAGAATATTAGAGTTGGCTTTTAATTCAACTTTTAATTTAATATTAGCTTCATTTTTACGTTGAAAATAATCTTCTTGGTTTTCTAATGGTAATAATGCAAAATTATTATTGATTGTCAAAGGTTGATTTTTTTCTTCTTTTGTATCATCATAAATAATATTAGTATCTAAATAACCAATATTTACAATTGCTTTAGGCGGCTTTTGATCAAATTCAATTTTATTAATAAAAGATGTTTTATTTAATTCTAAATTATCTAATCGAGCTGTTAAATATTTTTTATTATTTTCAGCCACTACATGAGCTTTTGTAATTTTATTATCATCATAATTAATAGTTGCACTCATATTTGTATTTAAAACATTGGCTTCATCACTAATTTCAAACTTTATATTTGTTGAATTAATACTATTTGGTGATCGCGGTTCTTCAACTAGTGAACTTGTTAAAACTTTAACTAAGGGTTTTTTAATAATCACTTTGTTAATTTCTAAAATATCATCTAAATTCGCAATATTGTTATTGACTGTTAGTTTATTATCAACAATTCCAATTAATGTATATTTATGATTTGGCGCAAATAAAGTATTATCATTTGTCTTTAGTGTTATTTTTTTATTAACATCTACTATTGCTTGTATTGTTTTTATTTCATTGGTATTATCAATTTTGAATCTAAGTTCAATCTTTTTATTAATTAAATTTGCATCGTTAAAATTTATTGTATAATCCCCGTTTTTTAAATAATTAACCACTATATTTGGTCTATTAAGAGTTTGTTTTTGATTGCTAGATAAATCAAAATTGTGTACGAATTCAATATTTTCATCACTAGCAGAAACAATTTTTTCTAATCGATACTGGTTTGGTTTTACTAATTTTCCAATATCAAAACAAATCTGACCATCATTATCAACTTTAGCTAATAATTTATGTAATTTATGATTTTGATCTTTAAAAATCCCATATACTTGTTGGTTAACATAATATGGCGCTACTTTAGCAATTAAATTAATATCACCATTTTTTGTATACTGATATGGTTGGTTTAAACTATTAACATTTAAATTGTTAATAGTTTTATTATGATATGCGATATTAACTGGATTAATAACTTTAACTAAAGGATGCGTGTTATTTTTATAAATGTTAACTAATTCATAGAAGTTATTATTTGCTAATTTACTTACATCAAAATCAACATTTCCTTCTACATCTACTTTAGCATCAATACTATAAATTTGATTATTTAAATCTTTTAGTTGCAAACTAAGAGAAGAATTAAATAATCACTTTCCTACATTTATTCGGTATATTTTTTGATTATTTTGGTTAATAAAAGAAGCTGTTTTAGGTGGTTTACTAATCATAATTTGATGTTCAATCGCTAACTCATTTTGTTGAATTAAAACGTGATTGTAATCAGTTTTGTCAAGAATTTTTACTAAACGATAATCATGATTATTTGCTAATTTTGATGTATTAAAATAAGCAACTTTATCTTTAATAGTTGGTTGTATGTAATGATATTGATGCTTTTCATCAACAAAAACACCCATAATTTGCGGATTCTTTTTAGCTTTGTTTTTTAAATTAACTTTAATAACAATATCACCAGTATTTGAATATTCATTGGTTTTATCTATCTTAGATAAAGTTAAACCAATTATGGAATTAGACAATTTATTTTTTTGTAATCGATAAACATTACCCTGTGGTAAATGGGTATTAATCTCGGTTTTAAGATCTTTTTTATTATGAATTTTTAATCAATAGGAATCATTATTTTGATCTACAAAAATAATTGAATTCTCCTTATCATAAAGTCTTAAAACAATTCCAACTATGACAGCTGATCAACATAGATTTAAACATATTAAAAAAGAAGCAATTTTAATATGCTTTTTTCAAAGTATTTTCTTATTATTTTTCATTTTCATATTAATATTTATTTTCTAAAACGATTCAACATAATTTAATTAAAACACATTTTTTTTTTTTTTTTTGAATTTATTTTGTGTAAATACCAATATTTCATAAATTACTACACTAAAACCCTTTTATAAAAAAATATTAAGCAAAAGCATATGAAACAATAATTCAACTTAAATAATAGTGCTAATCTAATATTTTAAATTGTGTTGGGAAGTTAAAAATTTAAAATATGATATCATTTATACAGTATTTTTGGACAATTTAAAAAAAGTAAAATTGTTGGAATTTAAAAATAAATAAAGGGAGAGTAGAAATATGATAAAAAAACATATTAAAAAAATAGTAATTGGAGCTTTAAGTACACTAGCGTTCATCACAACAACAGTTGTAGCAACAGCTTGTGCAAAACACAATAATTCTGTTACTAAAAATAATGAACCAAACGTTAATGTTAAAGATGAAATAAGTTCTTCTAATAGTGAATTAAATGATTCACATTATTATTGAAAAGCGCCTAAATCTGATACAAGCGAAGGTTTTCAAACAATCCATAAAAGTATGGTCGAAGATGGAAAACGTGCATTTTTATTACCAGGGTTTTCACAAAGCAATTATCTACAAGACGCAATAAAAAACGCTAAATTTGATAAAAATGCTGTTGGTTTTTTATTAGATACAGTTTATAATGCAAATAATGATCCTTCTTTATTTGATGGTGCAAATCGTGTTGCAAGTGTTTATTTTAAAGTTGATGATGCTGCTTTTTTAGGAGGGATTGCTGCCGCTTATATGCTAAATGCAAACCAAAACATTTTTGCAACAGATGGCCAACTTAACTGGGGAGGATATGTTGCTTTAAATGCTAAAAACACTACTAATTATTTAGCAGGATTTGCCTTGGGTATTGAATGAGCAAACAAGCATTTAGCAAACAAAAAAGTATTACAAGAAGGAAGTAGTGAGTCTAAAACATGAATCAAAGTAAATGAGGTTCAAGCTTCACAAAGTAGTGCTGGAGGATTTGATGAAAATAATGAAAATGCTAAAAGAATCCTTAAGGAACTAATTTCTAAAAAAGCTGATTTAATTTTACCAGTAGCAATTACCCAAATGAGTTTAGCAGTTAATGAAGCAATTAGCACAACTTCACACAAAGTAGCAATAATAGGCGTAGATACTGAACAAGAAAACGATAAGACAATTAATAAAGAAGCAAATACTTTTAAAAATACTAATATAAGTGGAAATAAAAATGGCGTTATTCGTTTTTCAATTGTTAAACGATTAGGAGTAGCAATTGAAAAATTACTAGAAAACGCTGTAAATGGTACTCAATTTCAAGGACATGTTAACAAAATTGGTGATGAAATTGATGTTCACGATAAATATAAATTAGGTGTAAACACTGTAGGTTCACTAGTTGATGGTGTAGTAGGAATCTCAGAATCTGCTTATCACTATTTAATTGATGCTTTTAATCTAGCACAAACAGATGAAACTAATAAAGTAAGTACATATGACCAATTAGTTCAAAAAATAATCCAAGATCCTTTATTTAAAAGTTTAAACCAAGATCCTAAAATTGATGGATATATAAACCCTAAAAATCTTGCTAACGCTAGGGGTAATGAAGGTGAATTAATAAATGGAAAAACAATTAGTCCAGAAGCAAATGGGGGAACCATTTATAAAGCAACAAATGGTTCATATTATTACTATCCTGTTGCTAAATCAACATATACTGCACAAAATGCCTCAACTATTTTTAAAGAAATATGAGATAAAGCTAAAAATGATGATGACAAACGTCGTTTAGTTGGTTTAATTTTATCCTTCGCAGGCGCAAATGTTAAAGATGGAGGATATTCTGAAATTAGTTATGATGGCATGTTAGGTTTTTACGCAAAACATGGTATTAAAATTCCAAAATTATAATCATCTAACATTTATATATAATTTTAATAAAACTATTCTGTTAACAAAATATATTCAGTATAAATATATCCATTTTTAATTTTTATAAAATCCAATACCTTAAGTATTAAGGTATTGGATTTTTGTGTTATATAAATCTATTTTTAGTAAATAAGCATTTAAAATACTATATAAAAGCGTATTTAAAATGTTTGTAGCACTTCAATAAAGAACAAATTTAATTTTTAATTAAAACAGATTTTATAATTTATAACTAAAAGTCTACCTTCTTTTTATAAAAGGTAGACAATTTAATTTATGATTTTTTCTAATGTTTATTTTTGAATAATTGTTACAATCAAATTATTTTTGTTTTTTTATCATTTGGGACTAATGCAATTTTTGCATTATCTTTTTTATCATTTGCTCTTCAATATTTAATACCATAAAATTGATATCTTGAAGATTTTGGCATTTTATCTAAAAGTAGTTGAAATTGGCCAGGAGTATTATAATTTTTGTTAACATTATTTTCAATTATTCCAACTGTTGTATTATAAATTTGACCATTATCTAAATTTTTGTACATAACTTCACCAAAAATAGAATCATTTTGATTATTCATATTATTAAAAATAAGTTTTCTAACACTTCCACTAAAACTAACATAAGTATAACTTGTATTATTTGTAGTATAAATGTTATGATCAACATTCGTAATATCTACTATTCCAGCATAATTACTTGTATTAGAACTTTTGTATATAACTGAACCATTTTTATTAGTATCATAAAAAATATTATCTCAATCATTATTTATGTTTTTATATAGGTAAGAATTTGGTTTATTTTCAAATTTTACAATTCTTAATTTATATTCATTATTTTGATCATTTATATTAATATTGTTAATATGAATTTGTAATTTAGGTTTACCATTGTCATTTACAATTTTAATTATTGGATTATCATCAATTTTATTGGTTGATAAATTTTCCACAATAGCACGAATTTTATATGAATTTAATAATGATTGAGAAAATCACTCATCATCATTAGTAATATTAAATTCCATTATTCTTGATCAAATTTGTTTTTTTTCATCAAAATTTGTAATATTTTTAAAATTAGTTAATTTTGTCACACTTGGTTCAACAACAAATGTTTTATCATTTAATTTACTAATATCTATTGAAGTAATATTATTAGTATTATGATTTATTTTAATAATATTTTTTAAAGTATATTCACGATTATAAGTTAAATTTTCAATATTAACGCTATAATCTTCTTGATTTTTATTTAAAGTTAAGCTTACTTCTTTTTTAGAAGTTGAATTATTGTATCTATACTCTAATACTAATCTATCACCATCATTGTAATCTTTTATATCCTTAATTTTTAATTTAATAATTCTTCTTCTTAAATCATTAGTTTTTTCATAAGTAATTGATTCGATATATGGATCAATACCTTTAGTTAAAATTTTATTTTGATTTTGTTCAATAATGTTAATGTTTGTGTTTAATTCTGTATCAAATAGATCATTGCCTGTTGGTCTTGATAATTTTATTGTAGATAAAATGTATTCTGTATTTGGCTCTAAATTAGATATTTCTCCTTCAAGAATCCATTTATTATCTTTTCTTACTAAATTAATTGTTTGTATAAATGTCTTGGTACTATCACTAACTTTTTTAAATACCAATTGGAATTTAGAATTTGTATTTAACATTGTTCCATCATTAGTAGTTGTAATTTCAAAAGCATACTTTGCACTATTAATTGTTGGTTGATCCCAATTTCCGTATTTTTTTGCATTATATATTGCTCAATTTGTTTTAAAATTAGTATTCTCTAACGTTCTTCTTTGTGCATCATTAATTGGTGAAATAATTTTATTACCATTTACATCTTCATAAGCTTTGTTTGGTTTTTTAATCATTCTTATTTCTTTTATGTAATATTCACTTCCTTCATCAAGATTTACTAAATCATGTTTAATATTTGCGTATAATCCATCATTTCTTGAATCAATGATTGACATTGTATAAACTTTCATTTTATCTTGATTGTTTGGATATTTTTTATTAGCAAAAGTTACAACTATCTTATCGCCATTTTCTAAAATTTTATCTAATGATTCTAAACCTAATCTAAATTTAGCAGTAGTTATGCCAATATTTGATTTTCACAATTGATTTTCATCGCCTTCTGTTTTAAATGTTACTGCAGTTGGTGCATGATGATATATAAATGAATCAGCAATATTATTTGAATTTAGCAATTCTTCAAAGATATTATTATTTGAAATTAAAATCTTTTTGAAAGAATATTTTCTATTAGGAGTTAGTTTACTAATATCAAAATTATAACTAGTTTTATTGTTTGATAAAGTTAGTTCATTAGTTTCTCATTCTTGATGATTGTTATCTTCATATACTAATTTAATTTTTTTGTTAATGTAATTAGAATTAATTCTTGAAAATTCTGCTTTTATTGTTCCTATCACTCTATTAGGATTAACAGCATTTTCTTGTATATTGTAATAAATATCATTATTTGATTCTAATTTCAATAATTTTAGTTTATCAACTAATGTTTTGAATTTAGTATCTAGATTACCTCCATATATTACATTATTTTTATTTATTGTTAACATTCATTTAGCTAAATCTGGCTTATTTTTAAATTTAGCTGATGTGAATCTATATTCTGTTTCTGGTTTTAAATCAGTTAAATTTCATTTAATTTTAGCACTATTTTTATTTGCACTTACTTCACTTAATACACCATCAATTTTTTTAATTTCTGATGGATTTGATGTAGATACAAATGTAGCTTCAACAATTTGATTATTTTCAAAAACTTGATCAGTTGAATTAACTTTTACATTTATAGTAGCACTATTCTCAGTTAAATTTGTTACCCAATTAGAATAATCGATAGATAAATTTGTATTATAAGGATTAATACCAAATTTATGACTAACACCATTAATACTTAAATCATTAAACACTGTATTAGTATCTTTTGAATCAGTAATTTGAGCTTTAAATAATTTATATTCACGATTAGTAATCAAATCCTTATCAAGAACTAAATCGTAAATCTTTTTATTATTTTCCAATGTAACTTCTTTTGTTCAAACAATTTGCTCATTATTATCCTTATAAGCTAATTTAATTTTTCTACCAGCATAATTATCATTAACACCAGAAACGGTTAATTTAATTGTTTGGGTTGTATTATTAATATTATCGTTTGAAGTAATATTTACAATAGTTGTTGGTCCTGATATCGTTCTGAATTTATAATTTTCATTATTATTAATAATAATATTTCCAGGTAATAAATTTTCATATGCTAAACGTGGTTTTTGAACAAATTCAACTTTTAATAATTTATATTAAGTTTGCTCATTTAATGTGGCCCCTGTTTCAACATTGATTGTAGCTACAGTTTTATTAGAATTAACTTCACTAATTTTTCTAGTCCATTGACTTATTTGACCATTATTTTTATTTTCAAGATATACGATAACTGATTGATTAGTTTCAAATATTTGATTCAACAGATTTTAATTTTATATTTAACTCAGCACTATTATACGTAATATTTAATGGAGTGCTCCATTCGATGCTTGTTGTTGATGGGTTTCGTTTAAAACTATTATTTACAGTATTTTGTTTTTTTAAATCAATATATTGAGAATTATTTTTATCATCATTAATTTGTATTTTAATAAAAGTGTAATTTCTATTAGCTATCAGACCACTTAGAGTAAAATTATATTTTTGCATACCTTTTTTTAAATTAACTATATTGCTTTCATATAATTCGTTATTTTGATCACGATAAATTAATTTTACTCGACGCCCATTATATTGATCATTTACTCCAGAAAATGTTGCATTAATTGTTTGAGAATCAACATTAATTTCATTTGTTGATAACAATTCATTTAAAATTGAAGGCCCAAATTGGGTTGTAAATGAAATATTTCTTTTATTTTCAATAGAAACATCTAAATCTTCATAAACATTGCTTGGTTTTGTAATATTAATTGAATTTAATACGTATGTTGAATTTGGTTCTAAATTATTTATTTTACCTTTAATTAATCATTTCCCATTTTCTTTTAATAAGTTAACATTTACTTGTTTTAAAGTACCACCATTTTTATTAAATGTTACCGTTGCTGTTGCATTTGCATCTAATTCTCCATCATTATCATCTGTTTTTAGAAGAAAATTAAATTCAGCACTTGTATCTGAAGGATTTGATCATTCGCCATTTTTAGAAATTATAGTGTGTGATTTGTTTATTTTAATTTGATAATTAATGTTATTTAATTTTGGTAATTTATTACTTTCAACAATTGTATTTGAATTATTATTTACTAAATAATACACATCAACTAATGAATATAACTTATTAGCTTTTAAATTATTTAAAGTAAATCTAATTGATGTCTTTCCGTCATTATTTACTAAAGATGACCCATTAATAACATTAGTTTCAATAATATCATTATTTGTTGATTTGAATTTAGCTATAAATCTTAAATTATGTGCTATTTTAGGATTAATTTGCAATCCAAGATCTACATCGATATTGTTTTTTTGATTAGCGTTTTTAACAACTATTTCTTTATTTATTGGACCATTAACAATAATATCGTTATTAATTTCTAATTTAGGTATTGAATCATTATTATAAATGATATTATTAGATTTATCAATTCCAATATTTTCAGCTGCTATACTTGGTTTATTATTAAAACTAATATTTACTATATTTACTTTATCATTAAAATTTAAATCAACTAAAGTAGCAGTTAAATATTTGATATTACCACTTACAGTAATAAATCCCATTGCTTGTTTATTATTACCATAAACAATAGTAGCTTGATCTTTATCTCTAAGTACATTATCTGGATCGTTTAAATTAATTTTAATAATAGTAGATTTTAAATTAGTTGAGGCTCGATTACTAATTTCTTCAATATTCAATACATTTATGGCGCTTGTTCTTGTTGTAATAAAACTATTTGTTAAATTACCTTCTTTTGGGAAATCTGAAACATTATTATTATCAATCAATTTTACATCAATTAAGGTATATGTCCGATTACGTTTTAAATTATTTAACAATATATTATATTGTGTTTTATTAGATTCAATTAATACCTCATTAGTATGAATTGATTCACTAGTATCATTTGATTTATAACTTAGTTTTATTTTCTTTCCAACTCATGATTTTTTAACTCCACTAAGTGTAAAATTAATTGTTTGCGAATTTGTGTTAACGTTATTACTTGATGTAATATTTATTAATTTGTGGTCGCCAACTTTCGTTGTAAATTCATAATTTGAATTAATAGAATTTGTGTTATCTAAAATAACATTATTTTCACTATTATTGATATTATTTTTGGCTTTTGTTGGTTTGTTAACAAATTGGATTTTAACTAATTTATAAGTTGTTTCTTCTTTAAAATTAACACTATTACTTAAATCATGCGCTCAAGTTCCTTCTTTGAAATCACTAGTTACATCTTTTAATGGTCTTGTGTATTGTAACCATGTATTAGTATCTCTAATTGTTTCTTTAGGAGCAAATCATGCAACTACTTGTTGATTATTTTCTAAAATTTTATCTTCTGATTTAATTTTGAAATTAAAATTAACACCACGCGTCCCAACAATTGTAGCACTACTATCATTTCATTGAACTGTAGTATTTTTTGTTTGAGTAATAAATGTATTTGACACACCATTTAATTTTTCAAGATCTTCAAAATTATTAGTATTAGAAATATGATTTATTTCAATTTTTTCAAACCTATATTCTCGATTTGAATTTAAATTTGATAACAATAATTGATAGTCATTTTTACCTTTTTGTAATGTAATTAAAGAACTTTCATAGATAACATTATTATTATCTTTATAAACTACTTTAATCTGACGTCCATTATATTTAGAGTTAACTCCTGATAACGTTACATTAATAGTTTGTTGAGTATCATTAATAGTATCATTTTGAGTTTGAATAACTTTTAAAACCGGATTTCCAGTTTCAGTAATTAAACTTATATTTTCTTTATCATTAATCGATACAGATAAATTAGTGTGAGTTTTTAATGGTTTACTTAATTCAATATTTTCTAATTTATATTTAGTTTCTGGATTTAAATTATCGATTTGACCTTTGATTAATCAATCATTATTTTCTTTAATTATACGAACTATTTTTTGCTTAATATTATTATGTTCATCTTTAAATTTAACAGTTGCATCAATGTTATTTAAAACATCATTATCATCTTGAGTTTCAATTTTAAATTCAAAATTAGCTGTTGTATCTGTAGGTGATTTTCAATTTCCATGTTTACTAATCATTGTAACACCTGGATTAATTTCAATTTCCTTAGATACATTATGGTCTTTAAATATTTTTCTTGTTTCATCAATATTTTGATGAATATTATCTAAATAATAAACATCAACTAATTTATATAATTGATTTGATTTTAACCCCTTAACACTAAAAAGAACTTCTTGTTTGCCTGTTTTATTAATATTATTACCATCAATTAATTCGCTATATACTTCATGACCAAATTCATTTCTAAATTTAAGCTTAAATTTAAGATTTTTAGCTATATGTTTGTTTATTGTAAATTCAATTGATGAATTAATGCTTTCTTTATCATTATAATTTTTAATTTCAAGATGTAAATTATTAGATGATAATGGACCACTTATTCTAAAATCATTGTTAATTTTTAATTTTCTAGTTTCTTCTATTAAATTTGTTAATTCATAAATTTTATTCGAATTATTAATGCCAACACTATCAACTGCCTTATTAGGACGAGTTATAAATTCAATTTTATTGATAATTGTATCATGATTAAATTCAAGATTAGATAAACTAACTTCTAGGTATTTCTCATTATTTACTGTAATAACTTTTGCATTTGTTTTTTTATTTGTATCATATGTTATTTCAGCAATTTCGTTGTTACTTAATACATCCTCAGGATCATTTAATGAAAATTGAATTTTTGCTACATTTAAATTATTTATTACTCGATCACTAATCTCATTTAGCTTATTGACACTAATTGGTGTTTTTCCTTTAATAATAAATGAATTTTGAAGATTTTGCTTATTTGGAAAATCAATTCAATTTTGACCATCAAATCATTTTATGGATTTCAAAGTATATTTGCGATTGTATTTTAAATTATTAAAATTAAAAACATAATTTAATTTATTTTCCTCTAAATTAACTTTCGCCTCTACTTCATCATTTGTATTAGATATATATACTAATTTAGCTTCTTTGTTAAATCATTCACTACGAATTCCATCAATTGTAACATTTACATTTTGCGAATTTGTTAGTGTTTGATTTGATTCAATATTTATAATTCCATAATTACCAGGTTTTGTTTTAAATGAAATATTAGTTGGATTAATAGTTTTAAGATTAGAGTTAGCATTTAGTGGCTTAGTTAATACAATAGACTTTAACTCATACTCTGTTTCTGGTTTTAAATTATTTACTATACCTTCAATAAACCAAATGTTTCCTTCTTTTTTTAATTTTACATTGTTAACAAATTTTGTATCATTTTGATTTGATTTTGATGAAAAGATAACATCAGCCATAACATTATTATCTAATATGTCTTCATCATCAGATTCAACTTGTAATTTAAAATTTACACTGTTTGAATTGATCTGCCATTCAACATTATTTTTTTTAAAAGTTGTATTACCGGGTTTAACTTCAATTTCATTAACAACATTATTAGATCTTATTAATTTATTATTATCTATAAGATCTTTATCGTCTTGATTTAATAAATAATATAAATTATCAAAGTGATATAAACGATTTGATTTTAGATTTGGAATTCTAAGTTCAATAATTTGTTTTTTACCTTCTTTTTTGATTGAACTATAAGAAACAATACTTGATTTTACGGTTTCATTATTTTGATCTTTAAATGTTGCTATAAAGTATAAATTTTGCAAAATATTATCATTAGCTTTTAATTCTAAATTAACTTTTACATTAGTTTTATTATTATATTCCAAAGTAATACGTTTATTAGTATCACTTTGAACAGGACCAATGACACTAAAATTATTATCAATTTTTAATGGATTTATATTGTTTGTATTGTTATATATTTTATTGCTATTATCTATTCCAATATTCGTAGCAGCTTTTATTGGTTTATTAATAAAAGTAATACTGTTAATAATAGTATCTTGGTTTAATGCTAGATTATTGAATGTTGTTTCTAAATATTTTTGACTCCCTACAATTTTAACTTTTGCTTTTACAGATAAATTCTCACCATAATTAATGATTGCTTCTTCTTCATTACTTAAAACATTATCAGGATCATTTAATATAAATCTAATTTGTGTTGATTGTAAATTATTTTTCGCTCTTGCAATTGGCTCAATTATTGAACTTATACTAATCGATTGTGTTTTATTAACAATAAATCAATCAGCTATTCCATTTTTTAAATGGAAAATTATTGATGTTTTATTATCATCATTAATAATTCTTACTTCTTTTAGTGTATATTTACGATTATGTTTTAAATTAGACAATTCGAAACTATAGTGGTTATTAGCCCAAAGCAATGTTTTTTGATCACTTAAAATTTCTTCACCGTCATTTGAAGTATATACCAATTGAATTTTTTTATTAATTCAAGTTCGTTGAATTCCATCAATATCAATATTAACTGTTTGTGTTGTAGTATTTATAGAATCATTAGAACTAACATTTGTTACTTTATGATCAACAATTAAGGTTTTAAAATTATAATTCGAATTATTGTCTTCAAAAATGACATTATTAGCATCATTATTAATATTAGTGTAAGCTAATGTTGGTTTTCTAACAAAGCCAACTTTTATTAGTTTATAATTTGCTTCTTCTTTTAGTGTTTGATCAGTATTAAAACTAACTGTTGCCTCACTTCCATCACTACTTATACTTGTGATAGTTTTTGTATATGATATTTCTTGACGTTCATCATCATTAGATTTCAAATAAGTAATAATGCTATCACCTACTTGAAATACGTGATCTTTAGATTTTAATTTATATGTAATTGTTGCACTTGTGCTTGTTATATTTGTTGCTAAATTAGTATTATCAATGACTATTTGTGTCTTTGATGCATTTACATTAAAACTATTCGTAACATTTGTTGAATTATTAAGTGTTTCAAAATTATTATTAGAAGTCTTTATTTTTATTTCTTTAAAAGAATATTGACGATTAGCAACACTAATTGGCAGTGTAAAAATATATTGTGTTTGATCTTTTTGAAGAGTTAGTTCATTTGATTCAACTAAACTTTTTTGATTATTACTTCTATATTCATAGACTAACTTAATCTTACGATTATTATATAAATCATTAACCCCTGATACTTTAGCGGTAATTGTTTGTTGATTTGATGCATTAGGTTGATCATTTCACATTAAATCATGAGATGTAATGCTAACTAATTGTGTTGGTCCACTTTGAGTTGTAAAACTAATTTGTTCTTTATTTTCAATTTGGATTTTTAAATTAGCAAAAGCATTCATTGGTTTAGTAATTGAGATGTCATCTAATTTGTATGTATCATTATAAGCCAAATCACTAATAGTATCTTCAATATACCATTGATTATCTTTTTGTTTTAGTTTTGTTGTAATTGTTTTTTTAGTATCATGTTGGTCACTAAAAGTGATAGTTGCATCTAGATCAGTACTAAAATCAACATTTTCATCTGAATTAATATTAAACTTAAATTTTTGAGCATTTGGTTGTGGGAATGTTCAATTACCATTTTTTTGAATCGTAGTAATCGTTGGTTTAACAGTTATTTTATAATCAACGTTTGAATTCTTTTCAAATTTATTATTTTCATCAATATTAGTTTGATTTGATGAATTTACATAATATAGTCCTTTGAAAATATATTGACGATTTGATGTTAAATTAGTTAAATTAAATGTTAATTCTTTTTTATTATTGTTATTATTTGTAACAATTTTATCTGCAGCAATTGGGTTTGTTAAAACACTCTGACCATCGTTACTGTCAAATTTTGCAATAAAATATAAGTTTTTACTAATATGATTATTTGTATCTAATTCTAATGAAACTGAAATATTTGTTTTATTATTAGCTTCAAACTCTTTTAGTGTATGAAGTGGGCCAGTGATTTTTAAATCATTATTAATTATTAAATTAGTAGCATCATAAATAACATTAGTATCATTAATACCAATATTCTTACTTGCATTTTTAGGTTTTGTATTAAATTCAATTTTATTAATGATAGTATCTTTGTTTAAGACTAAATTACTAAAAGTTGCTTCTAAATATTTTTGATTTTGATCAGTAATTACTTTTGCACTAGTAGTTTGATTATTATTATAAGTAATAGTTGCTTGTTCATCATTACTTAAAACATTATCTAAATCATTTAATTCAAACCGAATTTTTGCACTATTTAAATGATTTACATCTCTATCTTGAATTTCAATAATATTACCAACTCCAACTTGGTTATTACTTAAAACAATAAATTTATCTTGAATCGAATCACTTTTATTAAATGGTGTTTTAGTGTTATTATCATTAATTAATTCAATTTTAGTAAATGTATAAGTTCGGTTTTTCTCTAAATTAGTTAATTCAAACTCATATTCTAATTTATTTTTTTCTAATTTAATTATAGCGGTTTTTGTTGAGTTATCGTTTGCACTATAAGTTATTTCTAATTGTTTTTCATTTCATGCATTTTGTATACCATCAAGTTTTAACTTAATTCTTTGTTTTGTATCTACTGTTGATGGATTTTGTGATTTAATTTGAATAACTTTATAATTACCAGCTTGCGTTTGAAATGAAATATCGTCTTTATTTAATATTTCAACTACTAATGGCTTCTTTGTTTTATTTGGTTTAGCTAATAGAATTGATTCAAGTACATACCTGTTTTCTGGTTCTAAATTAGTAATTTGACCCTTAATTAAATATTTATTATTCTGCTTAATAATATTAACTTTAACTGGTGTTAGTAGAGTTTGACCTTTTTTAAAACTAATTGTTGCTTCTAAATTATCTAAAACTTCATTACCATCATCACTATTAATTACAAACTCAAATTGTGAACTTGTTGATGTAGTATTTCAAGTATTATTTGATTTGCTAATTGTTGTTTTACCAGGTGCAATGTCAATTATTCGAGTAACATTATTTGCTTTTGGTACTTTGTTTTTGTCATTAATAGTATCATTATTATCATCAATATAATAAACATCAACTAATCTATATAATTGATTTGATTTTAAATTATCTAAAGTAAAATTAATTACGTTTTTATTGTTATTAACTATAATTGAACTATTAGTTAAAATTGGACTATAAACAACTTCACCATTGATATTTTGAAATTTAAGTTTAAATTTCAAATTCTTACTAATATGTGGATTAACTTTAAAATCTAGTGTTGAAGAAATTACGTGTTTATTATTAGCCACTATATTTTGTGTACTATTAACATCAGTTGATAATGGACCAATGATTTGGAAATCATTATTAATAATTAATTTATTTAAATTACTTTCGTCATAAATTACATTTGTATTATTTTTGCCAACTTTAATAAAGGTTTGAGATAAATTTTTAAATTCAATTTTTTTAATAATTACATTTTTATTAAATACCAAATTTGTTATTATTGCTTCTAAATATTTTTGATTTTGTGCATTAGTTTTTACAATAGCATCAACTTTTTGTTCGTTATCATAAGTAATTGTTGCAATATCATTTGTTTTTAAAATATCATTGTCATCTTTTAAATTAATTTTAATAGTTGTTTGATTTAATTTATCAGGAGCACGATCATTAATTTCTTCTACGCTTGATGCTGTAATTTCGCTTTGTAAATTAACATCAAATGAATTATTTACATTGATTTCTTTTACAAATTCGTGATCTTGGCCATTGTCAACTTCTTTAATATTAATTTTCTTTAAACTATAACGTCGACCTGGTTTTAAATTATTTAATACTAAATTATATTCTTTTTTATCAAAACTTAGATGAACACTATTGTTGTTATTATCAACTGTTGTTTTAATTTCAGGATCACCTAAAATGTTTGATTCATAAACTAATTCAAGCTTTTTATTATTTCAAGCTCGCTGAATACCATCAATTTTAACAGTAATTTCTTGTTGTGTTGTATTTGTTGATGTGCTTGACACTACATCAATAACTTTATGTTCAAATTTTTGTGTCGTGAATTCATATGCTTGTGAACCATTTTTATACTCAAAAATAACACCATTTTTATTTAATAACTCATATGCTTTATTTGGTTTATTTTTAAATGTTACTTTGATTAAACGATATGTCGTTTCTTCTCTTAAACCGCTTAAATTAAATTCTAATTCACCTTCATTAAATGAATTAGTTACATTATTTAATTTTGCTTCAACTACTTTTTGATCATTTAAATTATTAGTTGGAGCAAAAACTGCTTCGACAATTTGATCGTTTTCTAATGCTTCATCATTTGTTTTAAGCTTAAAATGTACTTGAGCATTATGTGCACTAACTGTTTTAATAGCATTATTAGTATCATAATTAATTGTTGTTAATGATGGTTCAAATTCAATTTTTGGTTTATGAATCTTTGAATTAAAACTAATTTGATTATTTGTCATATCATTCACTGAATTTTGATCATCAAAATAATACAAACCTTCTAATTCATAAGCACGATTATGAATTAAATTAGAAAGTTTAAAACTAATTTTAGCATTATTATTGAATAAGATCGGATCAGTTCAAACAGTTTTATTATCGTTTAACTGCTTTAATTTTAAACGTAAATATTGGTTTGCTAATAATTGTTTATCAACTTGAAGATCAACATCAATATTTACTGATTTATCATTCTTGTTAATTGTTTGGGGTTTATAAGATGTTGGATTAATATTATTTGATAAACTAATTTTATTTTGTTCATCATATGAATAAACAATGTTATTAGTTTTATTATTAGTAAAATTAAAATATGCATTAGTTGGTTTTGAATTAAAACTAATTTCTTTAATAACATAATTTTGATTAATTTTTAAACTAACTAAATCGAATTTTAAAAATTTTTTATTGTTTTCTACAACAACTTGGGCATTAGTTGCTAATTTTAAATTATTATTGGCATCATTACTATTTTCTAATTGATAAACAATATTTGGTTTATCAGCTGTATTTAAAACATTATCAATGTCATCAACTTCAATCTTTGCTGAAATTAATGATTGGTCATTATTAACTAATATACGTTTTGTGATTTCAATATTAGTGTTATTTTTTAAAGAAACTGCATTATTACTTGGATTAATGCTAAATTGATGAGTTAACGTGTCTAACTTATGTAATGTTTGATTATTAGTTTCATAAACAATTTTAGAAAAAGTATATAAACGATTCTTAATTAAATTTGAAAATTCGAATTGATAATTTCTTTGTCCTTTGATTAAAGTAACTGCTTTTGATTTAATCTCTTTGTTATCATTTGATACATAAATTAATTTAACTTGTTGATTATCTTGAAAATCATCACCTATATTAATTTTGGCATTTATTATAGGAGTTTGATCATTATTTCAATTATCAATTGAATTTAGACCCACTAGTGTTGTGTTTTTATTAGATGTACTAAAATTAGTATTTGGATCTAGTAAATCAAATTCACTATTAACAATACTGCTTGCACTCTTATTTAAAGCTTCAAGTTTTTTAATAACATATGTTGTTTTTTCTTTTAAACCAACCAAATTGAATTTAATTTTATTATTTGCAACAACTTTTCCATATCCAATTTGTAGTTCGTTTTTAGTGTTATCAATATTTTGATAATAAATTTTAACATCTTCATTAATTCGTAATTTTTGATCATAATCTTTATAATCAATAAATAAATTAGCATGGTCTGCAAAAATATCTTGTTCTACAGCGCTTTTATTAAATGATAAATTGATCTTATGAGGGTTTGTAATAAAATGTTGTTCATATTTTGTAACATCATTTTTAAATAAATACACCTTTTGGTTTTGGTCATTTAAATAATATAAGTTTTTAATACTATATTTACTATTTCCTTTTAAATCAAGACCATTAATTGAAGCGATTTTATTAGTTTTACTAAAATTAACTAAAGGGTTAATAACATCTGTATCTAATAAATCATCATCCATTAAAGCTAACTGTGCATAGATTTTTTGATCATTTTTTAAAATATTATCTTTATCATCAATTTCAAAGGTGATAGTTGTATCATTATTAGTTTTAACAACCACATCAAATTTAGTTCAAATTGGTGGATTAATAGTAGTTGCTGAGCTTGTTGTGAATTCAAAATTTAAAACATCATCTTTAATTAATTTTATTGGTTTTTCTTTGTTGTTTAATTCTAGTAATTCAACATCAACAACTTTATATTTTGTGTTTGCATCTAAGTGTGTAAAATCAAAATTTAGTTTATAGTTGTTAGTTTTTGGATCATAAGTTGCAATGTATTTTTGTGTATCATTTAAATCATTAACTTTAGCAATTGTTAGTGCAAATTGACGATTATAAGATAGTTTATTTAAATCAAATAAGTTTACTTGGAAATTAATAGCATTTACTGTTACGTTAGAAACTGCTGGTGCTTTTAAAACAAGCTTACGAGCTTGATCGACACCATCATTAATTGCACTAACATCTTTGTTAATACTATTTCTATCTAATATTGTTTTGTTTGGATATTCATTTAAATCTACAATCTTATCAAAATCATAGATTTTGTTAGGATCAAATAAATTATTGTTTGGATTAACAATAACTTTTCCCTTATCATCAGTTTTGATTGGAACATGAATTTCATTGTTATTTTTATCTTTATAAACAGCAGTTAAATCTTTATTTTTTTGATCTGGTATTTTAAACTCATGCTTATTATCAGCATTAACATTACCATTTTTTTGTTTATTAACTATTCGATCTAATGGTGAAATTTCATCAATATTAGCAACTTTTTTATTATCAGCTTCAATTTTTGTTAGTGTATATTTATGATCTGGTGCAAAAATTGCATCATCACTTGTTTTAAAAATAGCTTTTCCATCTAAACCAATACTAGCTTCAACCGTTTTTGTATTAGTATTATTATCATCGGTTTTAAATGTTAATTTAATTTTTTTATTTGCTAAACTAGGATTTGAAAAACTAATTTCATAATCATCATTTTCTTTAGTTTTAGCAGTTATTGTTGGTTTTTTAAATGAAATATGATTATCACCACTTAATTGCTCTTCACTAATTAGATCAATATTTGGTTGATTATTTTGTTTTAAAACGACTTTTTTAAGTGTGTATATATTAGGTTTAGTTGGGTCATTATTTGCTAAATTTTTTGTTGGAATAATAACCTTGCCATTAGCATCAATACTTACATCTATCTCATGTTCTATACCATCTTGATCAACAACAATTCCTTTGACATTTGCATTATTTAAAGCTTTTTGTTTTAAATCATCATTTAAATCATTTGCTAATTGAGTGTGAATTTCTAAATTTCCATCATTATCGTACGTAAAATCTGGATGTGAATCTAATAAATTCAAATCTCGTTTATCAATTATTTTATCTTTTAGTTCAAAATCATTAAGATTAATGACTTTATTTAAATCGTTATTTTCAACTTTAGCTAAATGATAAATATATCCTTTTGGTAAATCATTACTATCAAAAATAATTTTATTATTTTGATCCACATTAGCAACTACTTTATATTCTTTATCATTATTATCAACAAAAGTTGCTACTAATTTCTGATTAATTAAATCATTATTTAAATTTTCTAAAATTTGGGTTTTTTTAGTTGAATCAACACTTGCATTAGCAGCTGGCTTTTTAATTAATTGTTTTTGTTTTGAAGTTAAATCAAAATTTGAAACCAAAACATTTTGTGGGTTTGAAACTGAAACAATTTTGTCTAGTGAATAATTACTGTTATTATTTAATGCACCTGTATCAAATGCTATGGTTCCATCTTTTTTAACTTTTGCTAAAATTTGGTGTTCTTGATTATTTTGATCTTTAAAAATTCCATATACTTGTTGGTTAACATAATATGGCGCTACTTTAGCAATTAAATTAATATCACCATTTTTTGTATATTGATATGGGGTATTTAATGCGTTTGAATTTAAATTATTAATTGATCGATTATGGTATGGAATCTGTTTAAGATTAACAACATCAACTTCATTAGTTTTTTTAATACCAATAACTTCATATAAATTATTATCACCTAAAGATGAAATATCAAAAACAGCTCGTCCTTTTTCATCTGTTTTTGCATTAATTTTATATGTTTGATGATTTAGATCTTCTAAGGTAAGTTCAAGAGGAGTGTTTTTTAATTGAACACCTAAATCAACTTCATATACTTTTTTGTTATCAATAAAATTAACTTTAGCTTTTGTTGGTTTATTTACACTAACTTTATGTTCAAGCATTAATTCATTATTTGGCACTAAAACATCATTTAATTCATTATTGTTAACAATTCGGTTTAATTCATATTTGTGATTTAAAGGTAGTTTTTTAGTATTAAAATACCCAACATTATTTTTTACACTCAACGCTGGTATTAAATATTCTTTATTATTTTCATCTTTAAAAACTGCTGTTGGCAAGGCTTGGTTTTTAGTTTTATTTGTTAAATCAACTTTTAAAACAAGATTACCTTCATTATCATAAGTATGTGTTTTATCAATTTTTTTAAACTGTTCATCAATATCATTAACATTTACAACTTTTGTTAATGGATTTGAATCTTTTAAAATTAGATCTTTTAAATAATAAACATTGCCATCTTTTAAACTACTTGTATCAATATCAATTTTTTGATCTAATCCAACATGTGCTTCAATACTATAAACTTCATTATTTTGATTTACAAATTTTGCGACTAAAATTTTATTAGCAAGTATTTTATTTACCTTTAATTGAATGTGCTTTTCGTTTTTTTGATCCTCATATACATTAGTTGGAACTGGTTTTAAAACTTCAATTTTCTGTTCAGGTTTTAGCTTCTCATTTTCACGAACAATGTTATTATTTAAATCAATAATTCGATTTAGCTTATATTTACCTGTTTTTGGTAAATACTTAGTATCAAATTCAAATTTTGCATAAGAATCAATTTGAGTTTTTATTTTATATTCTTGATTGTTTTCATCAACAAAAACACCATAAACATCTTTGTTAATATAAGTTTTTGGTAATTGACCTTTAATGATTAAGTCACCATCATCATTAAAACTATAAAAATCATTATTACTTAATTCATTATTGTTTTTTGAATGTAAAACAATCCCAATAATTGCTCCTGCTCACGCTAAATTTAGCGTTGCAAGCAATAAGAAAAGACGTTGCTTACGACTTTTTTTATTATTATCGTTTTTTATTGTTTGGATTTTTTTACTTTTTTTATTATTCATAAACGCTTCCTTTATGTACTATAAAAAATACTATTTTATAGCATTAATAACTGCAACTAAAAATGCTCATAAAATGATAGCAAAAAAAAAAAAAAAAGACCATTGATTGGCCTTTGTACTTATAAAACCCACTAAAACGTATACAAATTTTAGTAAAATGAAAAAACTTAAATTTTTATAAAAACTAGTTTGTGTAAAATCTTTGTGTAAAATATACTCTCATATCTTAGTTTACTTTAGGAATTTTATGACCATTAATTTTAATACTCTTTAAAAATTGTGTAGAGATCTGATTTGAATCAAGATTTGAGCATATATCTTTTAAATCATTTAATCAATAACAATTTTCATTAGTTAGTGTAATTTCAACACTTTTATTATTATTTTTATTAATTTTTGCAACTATTTTTGACTCTAAAGTATCCCCATCGCTATTGTTATATACATAATTTAATTCAATATTACATTCTTTATCTTGGGATAAATTACTTAAAAATATATCTTGATCAAAAGTAATATTAATTTTTGGATCAGATCCATTAACTTCATATTTAATATTATCTACTTTTCATTTTAAATTGGGATTTAAGATTCCAGGAAAATTAAAATAGCGTTGGTTATCTACTCAAGTTAAATCTAAATTTTTATCATTCAATTGAATTGAATTAATTCGATATTTAATATCAGGCTTTAAATATTTTAATTCAAATTCAATTGTTTGGTGCTCATAGTCGATTTTTAATTGGTTATGAGCATTTAAATCAATACATTCTTGTTGGTTGTTTTCATCTTCTAACACTAATGACATTTTATGGGCGTTTTTATCTTTAAAGATGAAATCGCTAAAATGCAACTTGAATTTAATGTTTTTTTGTTGTTGGTTAATTTCATGATCAATTTTATCTAAGTAAAATTGATCAAAGCCGTAATCTAAAGTTTTAAAAGTCTTATTATTGATATTTTTTAAATTAATCTGTTGCTTATCAATAAAAAGTTCACTAATTTCATAATCAGTATTAATGTTTAAGTTATCTAATTTAGTTGAGATTTGGTGTGTTTTTTTATCAAAATAAAAATCTGTTTTAAATAAATACAACAGCTTGTTTGTTTTTTTATTTTTTAAAATAACATCAACTCTTTTTGGCATATTTATAACTGCTTGGTTTAGTGTTAATTTTAAAGTACTATTTGTATTTTTAATATCATTAAATTCTAAATTTAATAATAAATGTGGAGATGATTCATTAAGTGTTTTAACTGTAATATTATTAGTTAAATAATTGTCATCATCTATTTCAAAAGAAATAAGTTTAAATGTTTGATTTTTATCTAAAGCAAAAAGATGTAAAATAATGTTTTTTGTTGCATTAAGTTGAGTAGTTTCTGATTTTGATAAGTTTTTAACAAACTTATATTTATTATGATTTTCATCTTCTAAAGTATATTTTATTTTATGCTCAATACTATCAGCATATTGATTGTCTTGTAAATCAACATTTAGATCGGTTGAAGTTGATTTTGTATTTGTGAATGAAAAAACAGCTTTTTTGAACTCATTTGTTTTAAATTTAAGAAATGAAAGATCCAAGTGAAATGGATGAGAATCAAAAAAATCTCCTATATAAACATTTACATTAAAATTATCACTATGGTAATTACCATCAACATCTTCTAAGATAAAACTTACAAATTCATAATTAATATGATTATCTAAATTTAATAATTTAATTTTAATTGTTTTTTGATCTTCATCAATACTCATGAAGCAATTTTTATCTTCAAAACTAAACTCCTTATATGCTCATTCATTTGGATTATTTAATGGATGATATCTTAATTTAATTTTGGGTTTTAAAGAATTTTCTTTTCTTAAAAGTAATTTTTCACATTTAATATTAATAATCGCACTATTTTTAGTAATATCCGAAATGAAAAGGTGTTCTTTATAATCTTTTTTTACTTCTTTTGATCTTTCATTATCATCTTTTTTAGAAACTGATAAATATCAAAACCAACTATTAAAAGCACTATAGTAAGAATTATAGAATGATTTATCTTTAAAAATTTCAATACTTGATCGATCAGGACGTATTTTAAATGTTTTGTTAATAGGTCCATAATATCCACCTGCATAGTCATCATCATTAGGGAAAGATACTGGTTTACCGTTTAGTAAAATATTATCAATTTCATAAGTTTTGTCAACATCTAAACCATTAATTACAGCAGTTAATAAACGTTCTTGTTCATCGTATTTAACTTCTGAGAATTCAATTGGGTTTTGATCATTTTCTTTTCTAATTAAAAATTTAAAATTATGAATATTAGGATTGTAGAAAGCAAGATCTTCAGATAATTTTACTTTAATTGTAACTGCTTTTGTAATTTTATCAACAGCTGTTTCATAATCAATAGCTTCAACTTTAGCATAATCGCCTTTTGTTGTAAAATCATGTGTTTGATTATTTAAATTTAAAACTTGATCATTAATCTTAATTTGTTTAATATCATAATGAGTTCCTCTTTCTAATTCATCAACATAAACAATAATTCTCTTGTTTAATTTATCAATAAAAAAATGCTTCTTATTAAGCTCTAATTGACCTTTTTTATAAACTCTATTATATGCTAAACTAATGTGGATATTATTTAAAGCTTCATCACTATCATCTTTTATTTTTAACTCTTTAAAATTTAAAGTTAAATTAGCACCATTATCATTAATCTTTGTAATTTCTAGATTTTTTAAAAGATTATCACTATCATCAAGGGTTTTAAAAGTGTGGTTTGGAATTGAAGTTAATTGAATTTTTTGATTATTAAAATTAAGTTTATCTAATAAATATTCACAATCAGATGTTAAATTATAAACGGTAGCATCAATTTCATTTTTTTGCTTATTGAAAACAGCATCACTAGTAATGATTTGGTGATTAGTTTTTTTGATAATTTTTAAACTAAAATTATGAATAGCATCGTCTTTAAAAGAAACTGTTTTTAACTTAATCTTTATATCAACTTGGTTTTGTTTAATATTTGTAAACGAAATATTATTAATTTCTAAATGATCAATCTTTGGTGTATTAGGAAGTGATGGTTGTAAATTTAAATCTTCATTAATATCTTGTTCGATAAAATTTAAATTATGATCAAAAATTAAATGTAGTGCAGGAAATGCATCATTTGAAAAATTAGTGATAAAATGATCATTAATTTTTGGTTTACGAGCTAGTCTTAAATAAATTTTGTTATTAATAATCAAAGGATGAATATTTTCTAAACGGTCAACTTTTTTATTTAACAATGGATTAAAAAGATTGGTGTTTAAAGTTAAATTCTTATAAATACCTTGCGAAGTATTTAGTTCATTAACTTCATAAACAACATAATAATGGTCACTCTTGGTATGAATTATATCTTTCATTAAAACATTAACATTTAACTTTGATTTATTGCTACAAGATGCAAGTGCTGTAGCAACAATAGGAACTAAAGCAGCTGTAAATAAAAATAATAACTTTTTCTTAATTAATTTCATAATTTTATATCCTTAATAATAACTAATTTTACACAAAACAATACGACATCATTATAACCCATAAACATCAATTCAAAAAAAAAAAAAAAACACTGCTTTTAATACCTTAATAAAAGCGGTGTTTAAATAAGTGAATAAATAAAAAATAATTGAATTTAGATTATTAAAAAAGCATATGCACATATAGTAAAATAATAAATAAAGTGCTGATCAAAAGCCAGAATTGTTTATTTTTGGTTTTAAAGTTTAGTTAATAAGTGGTGGTTAATTATGATGTTAATAATTAATATTGGTAGTAGTAGTTGAAAATTTTGCTTGTTTGATATGAATTTAAAACAAATATTTAGTGGCAAAATTCGCTTAGACGTTAGCCCACACCAACTAAAATATATCTTTGATAAAAATGAATATGTTTATTTACTTGATGATGATCACAACAATCACGTTGATTTAATCATTAAATTTTTAATTAACAAAAAAATTATTAAAAAATTAGAAGATATCACTTATTTTGGAATTCGAGCTGTTTATGTTAAAAATTTTTATGGAGCAAGTGCTTTTTTAAATAATGAAATTTATAATGATCTTAACCAACACACAGATTTATGTCCTATTCACAACCAAAATACTATCATGTTAATTAATGACATTAAGCATTTAAACACCTCTGCAAAAATTATTATTATTTTTGATAATTGGTTTCATCGTAGTATTAATAAAAATAAATACACTATTGCTATTGATCAAACAATAGCAATAAAACATAAAATTCGTAAATATGGTTTTCATGGAATTTCTTTTAGTTATAGTAATAAAATAATGCAAAATTATTTAGGAAAACAAGATGTTAATTTAATTATTTTACACTTAGGTTCTGGATCGTCAGTTTGTGCAATTAAAAATGGAAAATCATTTGATACCTCAATGTCTTACTCTCCTCTTTCAGGAATTATTATGAATACAAGAAGTGGTGATATAGACCCTAGTGTTTTACTTACTTTAAAAAAAGTAAAAAACCAATCACTGAAGCAATTGATCTTATTTTTAAATCAGCAATCAGGTCTTTATGGATTAACAAAACATCATAGTATTAGTGAAGTTGCTAATAATTTGCACAATAAAAATAATGAACTTGCCCTTGAGATGTACACTGATAGCATTGTTGATTATTTTATTAAGTATTTAAACCATTTACAAAAGATTGATGCAATTGTATTTTCGGGAGGAATTAGTGAATTTGAGCATCTTGTTTTAGAAAAAATTATTAGTAAAATTCATCTAATAAACCTTGAAATTAACGATCATTTAATACTAACAAACACGTTAAATAAAATTTCAACATCTAAATCACAAATTCCTATATATTTATGTTTAGTGAATGAAGAGCACGAAATTGCTTATGAAATGCTAAATTTAATTAATAAGACAAAATAAAAATTATTCATGATAGTCAGCACTATGAATAATAGAAAAATACTTATCTAATGATTTGTTAAATTCAATATTTTGATTATTTACTTTAACATCAACAAAATCATAAACAATTTGGTCCTCAATATTTTCAAATTTTGCTATTAAAGTTTTCTTATTTACATCATATTCCAAAGTTTTTGGGCTTATCAGATTAGTAGTGTATCCTGTTCGTTTTTTAATTGTAAATGTAAAATTATTAGATGAAGTGGGATTTGTGGGAATATTATCAAATTTTAATTTGATTGTTGTTAATTTAGTTTCTTTATTATAAGATTTACTAATTTCATTTACATTAAATTTAGTTTTATAATCACTATTTTTAGTATCAAAATCAAGCGGGATATTAAAAGGATGGTTATGATGATTTGTATTTTTTTTTAAAAATTCAGGTTTGTTTAATAATTGTTTATTTTTATAATCATTAATAACAAAGTCAATAACTTCATATGATTTATTTGATTCTAAATCATTAATTGTTACATTCAATATGTGGTTATGAACCAAACCCTCAACAGTATTATTAGTAGTATCAATACTATTAGATGATGGTTTTAGTCTATATTTGACACTAACTTTATCACCATCAACTAAATCAAAATCATCTTCTAAATGAAACTCTAATTTAGCGGATTTATCGGTTGAACTATAAGAAATCTTTTTGATTTTAATTTTTGCTTGAGGTGTATTAAAACTACCATTTAATCCTTGAGTATTTACTTCTAAAGCTTGTTTTTCACTAAAATCTTGTAGATCATCAAAAACTAGTTTAGAAAAAGTGTATTTTCGGTTTGATAAAACTTTATCGAAACTAAATTCGTACTCTTTTTTTGTGGGATCAAATAAAACAGGTTTTGATACAATGATGTTATTTGCATTATCCATGTATACTAATTTTAAATATTTATTTTTTAAATAATTAACGTTATTTTCAAAATATAATTTAATCTTAACACTCAAATTTTCATCATTAAATCAAAAATCACTGCTTTCATGTTCTAAGTTCTCTAATCTTTGAACAGTAGTTAATTTTGTAGAGGTTAAAATATTTAAATCTAAATTAGCTAATGATATTGGCTGTCCATTAAGAGTTAATGAATTTAATGAGTATTTTGTACTAGTTTGTAAACCCGTTAAATTAAATGATAAGGTCTTTTTAGAGTTATTGTAAACATAATTTTCAAAACTAAAAGTATTTGATGGTTGGTGTGCTGAAACTAATTTTAAAACAAATTTTTGATTATTTAAATCACCTAACTTATTATCTTTAAAATAAAAAATCAATTTTGCACTAGTATCATAAATTTCTTTTTCAATTTTGGTGATGTTTAAATCTTTAATAATCGGGTTTGAGTTTTGCTTATTAGTATTAGATAATACTAAATTTTGGTTACTAAAAACTAACTCTTTATTATTTAAAACAATCCTATGAATTTGGTATTTAGCGTTGTTTTCTAATTGAGTAAAATTAAATGTTATCGATGGCGAATTTGGTTGATAATTAAACTTAAATTTTGTAATTTGTTGGGTTTGTAAATTTAATAAACTAATCTCAAAAATATTTTGGTTTTCGTTCGCTAAAAAATATTTTGTAAATTCTAAATTTAAATCAGCACTATTATTAGTAATATTTTTTCAATCAATTTTTTTTATACTAAATTGATCTACATTTTCTTGTGTTAATGTACTTGTTATAAAATCTAGTTTAGTTATTAGCTGTTTTTGATTATTAATTAATAAATCTACTAATTCATAACCCATATTTGGTTTTAATTCATTAATAATTAATTTATTAAGATCATTATCCTTAACTAATTTTAAATTATTAATTGTTTTTAAATCACCAGTTGCTTTTTCTTTTAAAACAACACTGTATGAATGTGATGCTAAAACTGCACTACCTTCTAAAAATAACTCAATACTATTAGTAGTTGTTTTAAATTTTTTTATTTTAAAAAGCACTTGTTTATCATCAACCTTACTTTTTGATTTAGTGCATGCACCAATTAAGCAAGATAAACCAATAATTAAAGTACTACAAGTTAAAATTGTAATTATTTTAGTTAAATGTTTTTTAGTTATCATCTTTTATAAATCCTTTTTTGAAATTATCTATTAAAAAGCAAATTGAGCTTTTATATCATACAATAAATAATCTATTTTTAAAACTTTATTGTTAATCTACGATAAATTTAAAAAGGTACTTGGATGTTAAGTATAATAACTTTTGTGCTCTTTTTTTATAAATTTATGTATTTAAATCTCTTTATTAATTTTCATCAATAATATCATTTTCATCGATTTTAATATTAACAGCAACTAAAGTATGATCTGAGATAATAGAAGTAGCTTTTTTAATGTATACATCATTTGATTTTGCTATTTCATCCCAATTATCAACAATATTTTGCATTTGTTGATTACTTAAAGAACGAAAATAGGTTTTTAAAAAAGCTAATTTTCAGTTTTTATCTAATAATGTATTTGTTAAAGAAACTGATCATAAATCATACTTATAAGTATTTAAAACTTTAATTGATGGTGAAGTAAAAATTTTGTCATAGTGTTGTGCGTATTTTAAATAAGTTTTTGCTAATGTAGTTTTGTTATTTTCATTATCATCCTTAAAATTGGATAAATAATTACGTTTATAAAGTGAAGCAAAGGCTCCTAATTCGTTTTTTGTTTTAATATTAGTATCACCAACAAAAATTAAATCATCATTATTTCCATCTAATCTATCAATATAGTCCATCACATTTCCTAATTGATATGCCTCATTTAATTCCTTTGAGCCTTGTTTAATATTAATTTTTAAATTTTTATTACCATAAGTATGATCATGTTTGCTAGTTTTATGTTCGTTTGCTCCTGGTGAATCTAAATGACTAGCGACAATTGTAAAATTAAATTTGTTATCTAAAGTTTGAAATTCATAGGCATAAGGCGGACGTGAATAAGTAATTTTAGTATTATCATTCCAGTTCTTAAAATATTGTTCAAACAAAATATCTTTATTATCATTGTTTTTGTAAATATATTCTTGAACTGGTTTTATTATATTAGCTTTATAAAAGAAGCCAATTCGTTCAGTTTGTGATTCATTGCCTTTTGAAAATAAGTTTTTTGATAGCACATAATTTCAATTAGCTGTTTGATCAAATTGATTTTTGTGTAAATTTAATTCTTTTAAGATGGCACGCATTGGTAAATCATCGTGTTCATTATTTTTTGCACTATTAATTTCTGTTAAAGCAATTACATCAACATTACTTTTTAGAATCACCTTTGCTAAAGCTTCATTTTTTAACTGGTTTATTCCACCTTGATTTAAAACATTTCAATGACCTACACAAATTGTTTTTTGGTTTAAAAATGATCTTGTTGAGTTAGGTTGTTCTTTTAAAGAATTATTATTATTTTGAATTATATAACTATCATTATTTACACTTGTATCATTCTTTTTTTTATTTTTTAAATAAAAAACAACGCCAATAACTGCACTTGCTATTAAAATGATTAACAACGTTAACGAAATAGTAATTACTCATTTCCGGCTTTTATTATTCTTCATAAATTTCCTTAATTTTGTTCATAGTTATTAAATTTTAAACAATAATTTAATCCTTTTTTAATCTTTTAAACAAGGCAAAGCAAATGAATAATATAGAATTCATTTATTTATTTATTTATAAAAAATAAGTTTTTTTAAAAAAATTTTATCGTTAAGAATTAATTTTGTTTGGTAGTTTAATAGTTAAATTTTGATCATCTTTAGAAAAAGGACTAATATCATTTGTTGTTAAATCATTATAAATGATTGTTACTTTATTAAGTACTAAATCATCATAATACCAATCAAATGACTCATGATCATTTTGATCTTTATAGTCAATATTTGCATAATTTATTACATATGTTAAAGTTTTAGTTGCTTTATCTAAAACAAAATCTTTGTCCTTGTTTGAATTAATTGCTTCAGGATTGCTAAACGTTCTAGATAATGAAAATGTTCAATTAGCATTTTTATTATTTTTAGGCTTTAAAGACGAAATAATGGCAAACAGTTTATGATCCGCATTTTTGTTATATCATCAATTATTAGAAAACTGCAATGATATTTTTGTTTTTTTGGTTACTGAATCATAACTAGCAACAATTTTTTCTACCTTAGAAGCTGAAGCGTTAGTTTGAACCATTGTTATAGAAGTATTAATAATCACTGGTTTAGCATTTATCATTAACGAACTAATTTGATAAGTGCTCTCAGGTTCTAAACCTCCATAAATATTTTCATCATTATCTAAATCATAAATTAATTCAATTACATTTGAATTATTAACTTTTGAAGATAAAACAGTTTGGGGACTAAGTGTAAATTTTCGGTTAATAATATTAGTATCAAGATTATAAAAATTTAAAGCATAATCTGATGGATTTAAGCTTTGATGTAAACTTAAACTATCTAATTTCATTTTAATTTTTAATATATTATGGATTTTGTCATATTCTCAAGTATCAAGAGATGCTGTAATAGGAATCTCAAAAATAGCTTCATGATTGTGTTTTGATAATAACTCAATCACAGACTGATCATCGTTTATTAAACTAATACTTTTAATTACATATTGATGTTGTTTTAAATAATTTGGAAATGTGTTTTGGTTAATTTTTAATTTAAATCCTGCTTGTTGGAAACTAAATTCATTATTTAAACGATTAAAAATAATTTGCTTACCGTCTTTTAAAGTCAATTTAATTTTATTAATTTTTGTAAGATTAAGTTGTTTAAAATGTTTGATATTAAGATGTAATTCAGGATCATTCTTAGCATTAAATTTAATTACATTTGATTGGTATTCAGCAAATTTTAACGTAAATTGTTTATTTTGTAAATCATCAATTTCAATTGGTATTTCATTGATTGATGCTTCATTATTAACCACAGTTAATTTAGTAATTATATAATTAGAATCATTTTCAAGAATTTGACCTATCGATTTAGCATTCAAAGTTTTATTTAGATTTAATAGAAAATACTTATTTTTTACATCATAAAAAGAACCTGTTGTTATTTTGTTTTCTAAAATATCAAAACTAAAGGTCTTTATATCTTCTTGCTGATCTGTATTTATTTTTTTAATTGTTAATAAAACTTTTTTAGTATTTTGCACTATTGGCGAATCACTATCTAATTGAAATTTCATAAGCATATTTTGTGGATCAGTATCATCAAATGTAAATTTAATGATTTTAGAAAGATGTTTATTAGTTAAATCAAGCTGTTTTTCTCTAATACTTAATGGCAAAGTTTGCTTATCATTCATTATTGTTAATGATTTAATTTTAAAAAAAGGAGTTTTTAAAATAAGAGTTTGTAATTCTAAATTATTGACTAAATCAAAAACAATATTGTTTTTATCGTGATTAAACTTTAATGATTTTAAAATAATAGTTTTATTATTTTTAGTTGATATAAGTTCAATATCTGCACTTAACATATTATCTTTAAAATTATATGGGTTAATAAAATGATCATATGAAAGTTGTAATTGTTGTGGAATTCCATCATTTTTAATCACTTTAGTATCTAAAACATTTATTTTTGTTCATTTTGAAAAAACAGATGGAATTAATAAATAAGTTGGTCTTTGATTATTAATACTTCCATCTAACTGTAACCCACTTGGTTTATAAACGCTTTGGTTAATTTCAAGTTTTTTTTGGTCTAAAACTAATTTAGAAATAAAATATTCGCCATCATCTAATTGGTTATTTGCATCAGCATTTAAATTAGCAGTAATAGTTAATACTGGTTTTTTATTTTGTTGTGTTAAGTGATTAAATTGATATTTTAGAAAATGCAATTTAAAGGTTTTATTTGTTTTTTTATTTGTTAATTCAATTTCAATATTTTTATCTTTAATATCATTAAGATTTAAATTTAAAAGCTTAATTTCGAATTCAACTTCTGTTTTTTTAATGTTAGTAAATCGGGCACTCTGGTCAACAACTTTAATTAAATGATCTTCTTTATTAAAATTAAAATTAATGGATCTAGTCAACATTTTATTAGTTTGAATCACTTGAACGCCAACAAATTCTTGACTTGAATTTATAATAATTTTTTCATTGGGTTTAGGACGTTGGGGTAAGCGAATATAAATACGATTATTTATAATAATTGGTTTTATATCAACATAAAAAGCAATTTTTTGGTTTGAACTATTAATAATACTTGTTGAAAAAATAACTTTTGGCATTTTTTGCATATCTTCATTTGTTAATTTATTAAATTCATAAGCAATATAATAATCGTCATTTTCTGTTTTTAAAAAATAATCTAAAACTTTTAATTTATATCTGGTTTCACTAAAACTACATGCAGAAATGGTGCTGATAATAGGTATTGATATTAATGAAGCAATAAGTAAGTTTCAGTATTTATTTTTATTTTTTAATCTCATAGTTTATTCAATCCACTAAACAAATAAATATTTATATTTTATACCGGTTGTAATTAAGTTAAATTTAAATAAAAATTAATGCTATTTTTAGTTAAATTAAAAAATAATTTTTGGAACATTATATTGTGTAAATTTCCTATATTATTATAATATAAAAAAATACTTTAAGAACATTGGGAGGTTCTTAAAGTAAGAATTTAATTTTTATAAAAATTAATAATTAATTAAAGCATGAACTTTTCCACTTAGTTTTTCTTCACCTTTTAGAAATCCTAAACGAATTAAATAAACACTCCCTGCTACAATTCCTTTAGCTTGACGAACAAGATTTTCAATCGCAGCAACAGTCCCTCCTGTTGCTAATAAATCATCAACAATTAAAACGCGTTGGTTTGGTTTTAGTGCATCTTTGTGCATCTCCACACGAGAACTACCATATTCTAATGTGTACTCAGCACTATAAACATCATTTGGTAACTTATTTGGTTTACGTACCAAAATAAATGGCTTATTTAATTTGATTGATAATGGTGCTGCAAACAAAAAACCACGCGACTCCGCACCTATTATAGCATCTATATTTAATGATTTAGCAAAGTCAGCAAAATCATCAACAATCTTTTCAATAATTTTTGGTTCTAAAAATAAAGGAGTAATATCCTTAAAAACGATTCCTTTTTTAGGGAAGTCTGGTACATCCCTGATTTTTGATTTAATGTAATCAATATTGATCATAATCGTTATAAATCTTTCTTTTAAAATTTATTTATATTCATAATTTCTTGTTCATCAAATTTATCATAAACATTATCTGCATTATCATCCTTAGCTAATAAACCTTTACTAATATAAACCATTTTCATTCATAAATAATGAATAATTATGATTGATAAATAATGAGTAGATATTAAACTAAATAAAATAATTGCATTAAAACTAATTAAAACAAATGGTGTAAAAATCATTAATCACAATAATGCAAATAATATCACAATGTATATTATATTATAAGTATTAAAGTATTTAATTAAAGAATCTTGAATTTGAAATTGTAATTGTTTTTTTGTTACATGCTTCATTAAATCCAAAGTTCGTTTTAAATTTAAACAAATATGTAAACTAAAACTAGTCATTAAAACAAAACTCGTGTTAATTGCAATAATTGATATTTGATTATTAAATAAACGTAATAATCCAGCAATACCAAAGCTAATTAACGATGTACAAATGAGATTAATTATTATGGGGATTATTGATTGGGGTTTTAGTCAAATGCTTGATCAAATTAAAATAATAGCAAAGATAATAAAATACGCAGCAATTGAATTTGTAATATTATTAATTAAATGATATTGACTACTTCTATAAACAATTGCATCACTAAACCGGTGATTTAAACTGATCAAATCTATTTCACTTAAAACATTTGGTTTTTTAGCATCAAATTGCATTGATACTACTCCATCATTAATCTTATAATCTAAAATAGTTTTGAACTCGTGAGAATTAAAGTCTTTTAAATCACTTTTCATAATTAACTCATATACAGAACCAAAACTAAAACTATGGTTTGGAACAATAAAACCTAAAATAAATAAGCCAATAAAAGCTAAAATAAAGATAAAAATTAAAAAACTAATTCTATAAACCTTTTGATTCATGATTTTAAAAATCAATCGTTGGTATTTTTGTTGATCGTTTCATTCAAATTTATCATCAAAACGATTTTGGTTAATTGCATTAGTTGCAACTAAATATTTTCTTCATAGATGTAGTTTTGGAAGATGATGAGCAAGTGATCACAACATATACATCATTACAATAAATAATATATAAGATATTAACAAACAAGAAAAAGTTATTAAAATCAAATTAAAACCAAAAATACTAATGTTATTTTTACCAAAATACATAAAAACTAAACTGATAAAAATCATTAATAAATAAAACATTCCTTGATTAACAATCGTTTTATTTAAACTAATTCTTAAAGCGTTTTTATAAGAAAATTTTTGTTGAATTAAATGTTTTATATGGTTATTGAAATCAACTAAATTTAATAATGGCCAACAAATACCAATAATTAATGCACTATAAGTTTCTGTACTAAATCCTAAATTACTTGATTTTATTAATAATAAACTAATAACAAAACTAAAAGCCATTAACAAACTATAAATTAAACCTGGAATTTTATATAAAAGACTAATAATAATAGCGATGATTAAGATTAAAACTGCAATTCCAATCAAAATCGCATCCATAGGATTAATTGTTTTAATTACTGCACGTTTTAAATTAGATGGTTTTGTACTAAAATTATTTAATAAGTCATAAAAAGTTTTAGCGTAATCCTTTTTAATATTATTTTCACTAAAAACTGATTGTGCAAAATAAGTTGGTAAATAGTATTGTTTTAGATTATGAGCTGTATTAATAAAAGATTGAGTTGTATTATCTTTATTTAAAACTAGCTTGTCAGAGGTATATTTAACAATGCTTTGTTGGGCAGGAGTTAATTTATCATCTTTTTTTGGTGCTAGAAAGTAGTTAAAAAAATTATTATAATCAATTGCGTAAACAAAACCTCTTACTAAAGATAACTTATTAATGTTTCATGAATAAAACATTTCATTATCTTTAAAATTTTGTGTTTCATCTTGATTATTTGCATTTTTAATTTTTACTTTATGTGATGAAGATTGATAAAAAGCGCGAATTATATTCAATAATTGATCTGTTTGAACATTGATTGGTTCAATAATAGAGTTTGTATTAATAAAATATTTATCAAAATCTTGTTTTGCTAATCAATCAACAAAAGCTTTTTCCTCGCTGTTTGTATTTAAATTTTTATAAACAGTATCAACATAATCTCAACTTTTAGGATCAACATATTTTTGTTTTTGAGCATGCGCTAATGTCAATAACATATTAAATCTTGCAATTAATCCATCACGATTAACTCATAACAATCAAGAATTAACTGGAGCTTTTCGTTTTTGTTTTGTTCTATCAAAAGTCGCTACAGCTTGAGCTCTTGTTTTATTACCCATTGGATTGAAATTAAAATTAGCATCAAACTCTTTAGCAAAAAAATCTAAATTAAATTTTTGATGATCAAAACTAGTTTTTGGCAATAAAATTTCAACTTGCTGACCATTATTAGTTGTTTTAACACTAATTTTTTTAGGATCATTATTTAAAGTTAATCAATTTGTATTATCAAAATCCCTGTTTTGTGAAAGTTCAGGTTTTGGTGAAAAAAGTGAAGAAATGTTTTGTAATTCTAAACGGTTAGCGGTAGTAAATAAGCTTAAAAGTTGGATTTTTGAATTAATTTTTTCGTATGGCTTTGTTTTAGTTAGAAAATTAACGATATCTAATTTTGAATTTGTAGGATAAAATTGATAAACAATTTCCCCAAATTTTTCATGTTTTTTTGTATTTTTATTAATACGTTCATAAATAGCATATTTAGCACTAACATTATTTACACCCAATGCTTGTAAGTGTTTTTGACTTAGATTCGCTGTGGTTTTTAATAAATTTAACTGCTCTGCTTCTGTTTGAAAATCATTCAATAAAACTTGTGTTCTTGCTGATATTTTATCACCAAAATCACCAGTTTTTATATTCTTATTAGCATAATTAGCAGCCCCAATACCAGTGCCTACAATTGCTGCAATTGTTAATGTACTAATTCCAAAAAATGCTAAAACTTTTTTTCATTTCTGTTTGAATTTTATTTTTGTTTTAAATGACATTGTAAAACCAACTAACCTTGAATCATATTTTTAGAAATACTATAAACTCATAAAAACATTCATATTAACTAATTAATAATTATATATTAATTGTCATGATATCACTTATTAAATTCCTAATTATTTTTAAACTTGTACAAAAATTGTGTTTTTAACCTTAATATTTTTTATTTGCACTAATTTATTATTAAAAGAACCTTTATAAATTAATAACTAAAAACGTTTTAGATTATTTTAGAAAATAAATAATGGAGTAATATGTTAAGGTTATAATTATTTATAATTTAATTATATTAGAAAAAACAATTTAATAAGGATGTTATGCATTTTTTAATAACGAATGAAGTTAAAAAAATCTTTAATGATAAATTATATTCACAGCGTTTTAAACAAATAACCGATTTGGTCTCCACAAAATTAAATATTGATAAAAAGCGATTTTTTGAATGTCATTTTGTTGATGAAAAAACGATACAAGAAATTAATAAAAACTATCGTAATAAAGACTATATTACAGATGTTATTAGTTTTGCATTTGATGATGGCGAAATTATTACTCCTTTATTGGGAGAAATGTACATTTGCTATCAAAAGGTTGTTGATCAAGCAAGTCAATTTGCTCATTCATTCGAACGTGAACTATGCTTTTTATTTACACATGGTTTACTTCATTTATTAGGTTATGATCATATAAAAATTGAAGATGAAAAAATTATGTTTGCTTTACAAGATGAAATTTTAAATGAATTAAAAATTACAAGAAATATAAATGGTGCTAGAAATGATTAAAAAATACGGAATCGTAGCTATTGTTGGAAAACCAAATGTTGGTAAATCAACATTAATCAATGCGATTATGAAAAAAAAAGTCTCAATCATTTCAAATAAGCCGCAAACAACACGCAATGCTGTTAAAGAGATTTATGAAGATGATGAATCAGCAATTATTTTTACTGATACGCCTGGTTTTCATGAACCATCAAATAAACTAGATTTATTTTTAAATCATGAAATTGAAATTTCATATAAAGAAGCAAATGTAATCTTATTCGTGACAACTATGGACAAGGAATTAGATGCCAATGATTTTGAAATTATTAATTTAATTAAAGAAGCAAATAAAGAAAATATTATTTTAGTCATTTCAAAAGCTGAAATGGCTAAAAATCAAGATCAAATTGATGAAAGAATCCACTTTTTAAAAAAACATATAGCATTTAAGGATGTTGTTCAAATAAGTGCTTTACATGTTATTAACATTGATAAATTAATTAATACAATCAAAAATTACCTTCACAAAGATGTGGTAACCGATTATTTTCGTCAAAAAGCCGAAAAAGAAGATAAATTTGTTATTACTGAAATTATTCGTGAACAATGCTTATTAAATTTAAACCATGAAGTTCCTCACGGTGTTGGTGTAGAAATTGATGAAAGTAAATACAACCAAGAAGCAAATCATTGGATAATTAAAGCTTCAATAATAATTGAAAAAAATTCTCATAAACCTATTATTATTGGCCAAAATGGTACGATGATTAAAAAAATTAGCATGGGGGCAAGAAAGCAGTTGCATGAAATTTATGATTGTCATATTTCATTAACAATTTTTGTTAAAGTTGAAAACAATTGACGTGATAATAATAATATTATTAAATCATTAGGGTATAAAATTAAAAAATAATGGCTGAAATAATAACTAGAGGATATTTAGTTGCGCGCCAGGATTATGATGTTTTTGATGAAATATTAACATTTATTAATGAATATGGTAATCGTTTTATTATGTTTGCACCTGGAACAAAAAGAATTACTTCTAAGAACGCAAGAGCTTTGTTTTTTGGTAATTTTTTAGAAATACAATTTTTTTATGCAAGAAATGATACAAAACTTTCAAAATTAAAAAAAGTTATTTCTCTAGATCAAATTGATTATAAGTACGAAAATACATATTCAATGCTAATATTAAGCGAATTAATGACTAAAGTTGTTAATTTTAATGTTGAATTCTACCAATTCTATCAGCTAATACTAGAATATATCATTTTAGAATATAATGATTATTATATAAGCTGTTTTTTATTGGTAAAATTTTTAATAATGAATGGAGTTAATTTTAATTTTCAAGCATGTGTTCATTGTAATAGCTCAAAAAATATTAAAACTTTTTCTTTGGCTGATCATGGTCTTATATGTATTAACTGTGAATCTAAAATTATTAATAAAATAGATTATGAACCAAAGGCGTTACAATTATGGCAGAAACTTTATTTTGCAACACAGATAAAAAAAGACGAGGATAAAGATAATGAAATTATATATAAGGGGTTATTAAAAATATTAAATTCTGTTCTTTATGATCAATTAGGAATTTATTTAACGATAATAAAAAATATTTAAATAAAAAAGGATTTTAAAAAATAGGCAATAATTATGAGATATAAATTTAAAACACAAGAAGACTTAGTAAATCATTTAAAATCTGTTGGTTTTGTTTTTGCAAATTCAGAAATATATAATGGATTAGCTAACGCATGAGATTATGGACCATTAGGTGTATTATTAAAAAATAATTTAAAAAATTTGTGGTGGAAGGAATTTGTTACTAAACAAAAAAACATTGTAGGTTTAGATAGTGCCATCATTTTAAACCCCTTAGTATGAAAAGCAAGTGGACATTTAGATAATTTTTCAGACCCATTAATTGATTGTAAAAGTTGTAAAACAAGATATCGTGCTGACAAATTGATTGAATCTTTTAATAAAGATATTCATATTGCTGAAAATTCTACTAATGAAGAATTTATGAAAGTTTTAAATGATCATAAGATCTTCTGTCCTACATGCAAACAATTTAATTGAACTGATATTCGTCATTTTAACCTTATGTTTAAAACACACCAAGGAGTAATTGAAGATCCTAAAAACATCGTTTATTTAAGACCAGAAACCGCTCAAGGTATTTTTGTTAACTTTAAAAATGTTCAGCGATCAATGCGTTTACACTTACCATTTGGGATTGCACAAATAGGTAAATCATTTAGAAATGAAATAACTCCTGGTAATTTTATTTTTAGAACTCGTGAATTTGAACAAATGGAAATTGAATTTTTCTTAAAAGAAGAAATTGCATATGATGTTTTTGATAAATATTTAAACCAAATTCAAAATTGATTAGTTACTTGTTGCGGATTAGATTTGAATAATTTAAGAAAACATGAACATCCCAAAGAAGAATTATCACACTATTCAAAAAAAACAATTGATTTTGAATATAATTTCTTACATGGTTTTTCTGAATTATATGGAATTGCATACCGAACTAATTATGATTTATCTGTGCATATGAATTTATCAAAAAAAGATTTAACATATTTTGATGAAGAAACTAAGCAAAAATATATTCCTCATGTTATTGAACCATCAGTTGGTGTAGAAAGATTATTGTATGCTATTTTAACTGAGGCTACATTTATTGAAAAACTAGCAAATGATGAAGACCGAATTTTAATGAATTTAAAATATGATCTAGCCCCTTATAAAATTGCTGTAATGCCATTAGTTAATAAATTAAAAGAAAAAGCAGAAGCAGTTTATAATAAAATTTTGGATTTAAATATTTCAGTAACTTTTGATAATAGCGGAAGTATAGGCAAACGCTATCGCCGTCAAGATGCTATAGGAACAATTTATTGTATAACAATTGATTATGATTCATTTGGTGATGAACACGATCCTACTTTTACTATTCGTGAACGAAACACAATGGCTCAAAAAAGAATTAAATTATCAGAGTTATCTTTATATTTAAGCCAAAAAGCTCATGAAGATTTTCAAAAGCAATGTCAAAAATAACTAACAATGTTTTTTTAACAATTCGTAATGCTATAAATATTTCCGATGTTATTCAAAATTACATTAAAGTAATTAAAAAGGGTAATAATTATTGAGCTATTTGTCCATTTCACAATGATACAAATGAATCATTGTCAATCAATGATAAAAAACAAATCTTTAAATGTTTTGCTTGTAATCATGCTGGTGATGTGATTAAATTTGTTGCTGAATATAAAAAGATCTCCTATGCTTTAGCAGCACAAGAGATTGTTCAATTAATGAATTTAGATTTAAACTTATTGAATCATTTACAAAAAATTTCTCCACAAGAAATTAAAAAAAATAAGGTTTTTGATTTAAACAAACAAATTCAAAAATGATTTATTAATTTTTTAAATAATAAAAATAACATTCATGTTATTGATTATTTAACTAAACGAAATTTAAATAAAAATGATTTAGCATATTTTAAAATCGGTTATGCTCCAAATAATGATGAATTATTAAATTTAATAAAATCTAATTATAATAATCTGATACAAGAAAATGACGAGTTTGAATTAAATAAATTAATAGAAAATTCCTTTTTAGTAATTGATAAGAATGGTAATTACCATGATTTTTTTAATGATCGTATTATTTTTAGTATTTATGATCATCTCAATAACGTTGTTGGTTTTAGCGGACGTATAATTACAAATGAGAAAACACCAAAATATTTAAATACTAAAACCACTGCTGTATTTAAAAAAGATGAAGTTCTTTATAATTTTCATAATGTTATCACCATTGAAAATAATCATCAATTATTTATAGTTGAAGGTTTTATGGATGTTATTACAATTCATAAATCTAACAAACCAAACGTTGTAGCAACAATGGGTGTTGAATTAACTAATAAACATTTAGAATTAATTAAAAGCTATGGAATTAAAGATTTAATTTTATGTTTTGATAACGATCAAGCAGGTTTTTTAGCTACTAAAAAACAAATTATGAAATTAATTAATTCCCCATTCAACATTTTTATTGTTAATCATAAAGATAATGATTGCAAAGACATGGATGAATATGCAAATAAGCACGGTTATAGTGCAACAAGTAAGCTATTAGACCAACAACTTCATATTAGTGAATTTTTATTATTAGAAATTAGTAATACAAACAAATCTACCCCCTCACTAAAACACAGTTATAAACAAGAGTGTTTTACAATCATTAAAAAATATGGTGATATTTTTTATGTAGATAAATATGTTGATTTCATGAAACAAACATTTTCAATTGAAGAAAATTTATTAAAAAAAGTTATTGAAGAATTATTAATTAACAAACAATGTAATTCTATTCAAAACAAAAAGAACATTCAATCATATCAAAAACAAACACAACTAACTAATAAACTTACTACTTCAAACCCAAAAGTTATCCTAACAAAAATTGATAAACATTTTAACCACTTAGTAGTAATTATATTAATGAATCGGGTTTTCTTAAACCGTCTTGATACGCAAGTTCGTAACCGGTTAACTAAAGAGCAAAGTTTAGTGATTAATATTATAGAAGACTTTTATTTGTACCATTTTAAGATTAATAATATTTTAAAAAACACTGATTCTTTTATTGAATTTATTAATAAAAACTACAGTAAATATACTAATGTTATTAACCAACTTACAAAAATGGTGCAGATTATTAAATATAACCCTAAGTTTGATAATATAGTGGCATTTGAACTAGCTAAAAATAATTTTATATATGAATGATTAAACAACCAAATTAAACGTTTAAAACTCATCAATCTAAATTTAAATATGAATAATAACCAAGAACAAATCATAAATAATGAAAAATTAATAAAGCAATATAATGATGAAAAAATTTTATTAGACAATTTAATCAATGCTTCTTTATTTCATAAAAATCAATAATGAATACAAAAAAGCTTTTTAGTAATGTTTGTTTACTAGAAAGCTTTTTTTATTATTAATCAATTAGTTTAAATGTAATTAAAATTGGTTTTCGTTATTTGTATCCATAGCATCATTATTCGTTGTACCAAAACTATCAGTGTAGTGTTTGTGTTGATCATCAATTTTAAAGATTGATGCTTTTGGTTCTCCTGAATAATATGTAGCGTTTGAACTATTTGGAAGATTTGGATTAATTCGTGGTTCATATCACACTTTACCAATATCAATTGTATTTTTTAAACTATCTGAATTTCTTCGAAGGTAATTTGCTAATGTACAACTGCTTGATAACACGTTAGCACTTGTTGGTCGTGAAGCTCTTTCAAAATCTGTTAGTAATGAAGTATCTTCACCTTCATTAATACCATCAATATCATAGCCTTTAAAAGCTAAATTATTTGTATTATGTTGATAAATTCGAGCATAAACACCAAGTGATTTAGCTAATCCTAAATCAAAGATTGTTTTACGATAACCACCAAAACCTGTAAATGGTCCTTTAAGGGATAAAGCAACTTGATATCTTCAAGTAAAGTTATTTCTTCCATGACCAAATTTTCATACTTTTCAATCGACTGATTTGGTACTAAATGCATTTTTTAAACTATAGCCCATATCTTTAGCTGAAATTGTATTTGATGCTTGATAGTGTAAGACTTTAACTATATTATAATTTTGGTTTGGAATTAATTGAATATTTTTAAATTCTAAACAATTATTATGTGCAACAATTGAAACAATCACGTAATCTTTTTTAATATCATAGTTTAGATGATTATCAATCTTATTTTCATAATCACGTGTATTAAATGGATCATCTCATGTTGATAAAACTACATTATAAGTTTGTCCTTCAACAAAAGTATTTGCTTCTTTACCTTTTAATTTAAAATTAACCACTGATGCTGGGTAACCACCAGAATCACCTTTTGTAAAGACAATATCGTTTTTAACAACTGTAGTTTGATCAAATTCAATTTGAGCCCCTACGGTTTTAATGTGATATTTTGATGAATTATAATTATTAGTTGTGTAAATAATTTGATTATCAGCATTATTTATTGATCCAATAATTCTATTAATACCTTTATTAACAAATGAAATACTTTTAATATAATAATCTTGATTAAAATTTAAATTGTTAAAATCTGCATTGAAATATCAAAATGGGTCATTATTATTTTTCTTAAATAATTTACATGCTCCAGTACTAAAACTAGCTTCATTATCAGGTTGATCTTTTCGTTTGTATTCAATATTTATAGAAAAATCACCATTTAAAATTTGGTTTAAAACTGTACTATCATTTTTTCCAACTAATTTAAATTGTTTTGAAATTCCATCTTTAAATTGCGCAAGATTTTGCACTTCATTAACATCAATATGAATACCTGTTTTAATAGTTTTTGTTATGCGATTATCAATATTAATTATTTTGAGATTATCGATCATATAAATTTGCTTTTCAATCTCATTTTTATTTGGATGAGTTTGCTTTTCATTATAATAAACACCTAAAAATTGATATTCACTATTTTCTTCTAAAGCCCCATTATTAGGTAAATCAAATTCTAATTTTGAATAATTATTAGTATTTAATTGAATTGGTTTAGATCAAACTTCTGCATTTGTTTTAGTGTTAGTAAATAAAGCAAACATGTATTTATTATAAAGATGTTCATCTTTATTGTTAAATGTCACAGTTGATTTTAGTGTTAGATTTTTATTATCTAATTTATTTTTCACACTTTTTACTTCATAAGGATTTTGGTTTTCTTCAATTTTAATACTTGAGTATTGACTATTGTCTAATTCAAAAGTTACTTTATCCTTATACATTTTTGTTTGTGGATTTTCATACATTAATTTATTTAAATCCTTATGGGATAGTGTCTTAGGTTCATAAACTAAATCAATGCTAATAAGTTGATATAAATTACCTGGTTTAAAATGATCATCATTATTATCAATATTAAATTCAATGATTTTTTTCTTGTTATCAACTTCACCAACTAATGGAGGGTTCAAAGGTATTTGTTTTCTTTTATTTGGCAGTAAAATTTTTTCTATACCTATTCTTTGCCCCATAAATAAACTGTCATCTGGATCGTCATATCGAATTTGAAATGTCATTTTTTTATGATTTTTATTATAAACAATCTTAGGGGTTGCAACATATCATTTAATTGTTGTTGCGTCAGTACTAAATGATAGTGCATTACTATTTACATTTTTGAATGCTTCACTATCAGTATCTTTAGATATTAGTTTTTTATGGTGTTTATCTTGAGAAAAACTAGGATCTGCTCATTCAATTCCTAATATTTTATATTCACGATTACCCTTAAAATATTCATTATGATCTTTAATGATTTGAACTAAATAGTCTTTTTGACCCGCTTTAAAATTTATTGGTTCAGAATAGATTAGTTGTTTATCATTAGATTCTCAATTATTATTCTTTTCATCCCACACATCACTTTTTCTTTTTGAAGATTCGTAAATTAATCTGAATGGTTTACCAATATAAGTTCCATCATGCTGTAATTTTAAATCAAAACTTATACCATCATTAGTTTGATCTTTTTTAACTAAACTAATAATTTTTGGTTCAACAGTTTTAGTTTTGAATGAAATATTAGGTTGTGTCTTATCATTAGTATTTTCATAAATTACATTGTTATTAGCTTTTGAATTTAAATTAGCATAGGCCTTAGTTGGTTTTTGCTCAAATCACATTTTAGTAATTTTATATTTAATATTAGGCTCTAAATTATTTAAAGATAAAAGTACAATTGCACTATTAGAATTTCCATTTAATGTGTATGTACGTTCAAATACTGGAATATTTTCTGCTGGAATATCTTTTTTATTAATATCTTCATTAAATGGTGTAATTTGAATATGTAATACTTGTTTATCAAATACTTGATCTTTTGATTCTAATTGAAATCAAACTGTTGCAGCATCAGTTGTAGCTGGTGTTTTAATATTTTGAATTCGTACACCATCGGTTGGCACTAATAAATTTGCTGGGTATAAACTTAGTAAATATGTAGCATTACCTTGTTCAAAATCACTTTGTTGATTTCCAAAATATAAACCATTTAAATTATAATAACGATTTGTAGTTAAATTATCAATCTCAGCTTGATAAAGAATTTGATTATTATCATTAGTTGTTATTAAACGGGGTTTAGTTCATTTTGTTTCCCCATTATTATCAACATATGCTGCACTAATTCATTTATTAGCTAATAATTTTGGAGCTTGTGTAATTGTAATATCAACCTTACCTTTATTATTAACAACATCACCTGTTTTTGATGGACGTTCCTTAGTTGACAAATCATTATTTTCTAATAGAATTTTAAATTTATATTGATTATCATCATTTGTTGAATTTTGATAAATAGATGAATAGTTGTTTTTATCATTATTATTGATGTTTTCACCCATTCTAGCTGGTTTTGTTAACAACATTAGTTCACAAACTTTATAATCTTGATTTAGATCAATTCCACTAATATGAAATTTAACTATTTTTTTATTTTCTTTAATTTCAACATGTGCACTAGCTGAAACGCTTTGTTTTAGATTATTTAACATTGCGTATTTAATACTAAATTGATCTTGATTAGTTAATATATTACTTGGATCATTCAACTCAAAACATAAGTCTAAAGCCCCTAATGCTTTTTTAACTTTATTTCCATTTTCATTAACTTCTTGTGTTAATAGTGAATAATTAGTTGGAGTTTGCATTTGATTAATACTAATACGTTGAGCATTTGGTGTTTTAATATTTCCTTTAGCAGTTTTTTCTAATGCTAATCATGTTCATTTACTATCATCAGTGGCTTTATTATCATCCCCATATCCAATACGTAATGTATAAGATGAATTTCATAATAAATCACTACCAAAATCTTTTTTTCAATTTAAATTATTAAGATCTAATGAAGATATTGGAATAGTGTAATAAAGTGGTTTAGTATTTGGATCACTATTATTTAATTTTCACTCAAAACGAATATATTGACTTTTACTAAATACACTTAAATCCCCATTTAGTTTAAGATCAATTGTAGCGTTTGGAGTATTATTTACAAAATTAATCGTTGCAGTTTCAACATAAGCACTTTTCATTGTTTTAAACACTATTTGATCAGAATCAACATTTAAATTATCAACTGCTGTTACCTTGTTAAATTTCTTCAATTCTTCTTCATTAAATATTAATTTAGCATCTTTTAGTTCATATTGCATATCTTCAGTTAAGTTATCAAAAACAATTTCATCTAAAACAATTTCATTATTTACTATTTTAGTTTTTAGTTTTTTAGTAATTCATGAATTAGATGATTGATTATTTTTTACTCCAAAACGTAATTCAAGCTCATAATTTAAATTTTTTAAAACACTATCTTGGTCTTCAAGTTTTAGATTTAGTTTTGCATGATGTGCTTTGATTTCACTAACATTTACTTGCTTAATTTTTAAAGGTTTACTTGCACGAACTGTAAATTGATCTAAATCATCTGTTTTCTTTGTTAGATAACCCTTTTCTAATTTATATTTTTTAAAATCAGTATTATCACTCACATTATTTTTTGCTAAACCCACTCATTTAATTGTATATTGCATTCCCCATGTTAAATTATTAAGGTCAAAAGTAAAGTATTTAACAGCATTATTATCTTGATTTTTATCTGTATCTTTATGTGATTTTGTATAATTAAACTCTTCGTTTTTATTATTTACTAATAATAAACGTAAATACCCTTGTTTAATAAATTCAGAATAATCACCATCTAAAGTTAATTTTAGGGTATAACTGTTTTCTTGAGCTTTTTTGTTAATCTCAATTTTATTAATAAACAAGTGTTTTATAGTTGCAAAATGAACATTGTTAATTTCAAAATGAGGTTTTGAAGAAATATAAGTTTCTTTTTGACTTCAATTTTCTTGATTTAAACTAACCGTTTTTAGTTCATATTCTGTATTTTCACTTAATCCGCTAAGATCTACATCATTTAAAATTACTTCATTATTTTGAATTTCTTTAACGCTAACATGTATGTTTTTTTGTTTTGGAGTAGTTGGATTTATTTTTGTATTAAACACAAGATCTAATTCATCGCCAACTTTAAAAATATGGTCAGGATCATTAAGTGTTAATTTTAATTTTGCTGTGTTATCACTAAGATTACTAATCTTATCATTTGCATTAATATAACTTATAGTATTGATTGCATTTGTTAGTACTTTTATTTGTGGTGGATTTAAATTTGTGATATTATTAGCAACAAAAACTTCTTTGTTTTGATCATCAACACAATAAACACCAACATAACTATATATGTGATTTTTAGCTAAATTTGTAAATGTAAAATCTAAATCGTCATGAATATTTTCGTCGATTTTAATTTTAGTTGAAGATCAAACTAATTCGTTAGTATCATTACGTTTATATTTTAATGCTAAATATTTACCTTTAAAAGCACTCTTCATGTTATTTTGCATTTTAACATGAACTGTCGTATTACTATCAGTTGGAGTTGAAGGGTGTGCAACATTAATACTATTAACTTTAAATATGTTTCCTACAAGATAATTTTGATTTTTAATATCTTTATTATAAATAAAATTATCATAACCGTGATTTGTCTTGTGAATATCAAATTTCGCTTTTGCTGGTTTTTTATTAACTGATACAGCCCCAACTTCAAATTCTTGGTTATTTAAACTACCAACACTTAATCAAACTTCAGTGTATCATTTATTTTCTTGTTGGTCAAAATGAATAGGTCAATCATGAGATACTCATACATTGTTTGTATTTGGATAAACTAACTTAACATTAACATTATCATTATCATTAAAGATTTGATCTTGATTTAAGTCATTAAAATAAATTCTAAATTTAACATCGTTTTGTGTTCTTTTTGAATCATCTTCAACAATTTTTGCAATACTAATTGGTGTAGGTTCAACATTAAATTGGTCAACAAGATTATTCTTATTTAATTCACACCATTGGTTATGTTCATTTAAATATAAAATTTTATCTAAATGATATTCACGGTTACCAATAATTGAATTTGAAGCGTTTTTATCAAAACTAAACACATAATCATGTGTATTTGTGCTAACTAATAATGGTTGAGTTTCAAAGGTTTCGTTATTATTTTGTGATTGATAAACTAATTTTAGTTTTTTAGCATTTAAATATGGATCAATTTTATTAAATTTAGCGTTAACTTTAATTTCTTCTTTATTTGATGCGTAAGTATTTTTTATAGTTTTAATACTATTAATTTCAAAAATAGGTGTACTTGTTGTAAAAATAATTGAATGTGTTTGATCATTTGAATAAAACTGATCATTATATTTGTTATTAATATGTTTATAAGCATTATTTGGTTGTTGTTTAAATTCAACACTAACTAATATATATTTAGTATTTGGTTCTAAATTATTTAACGTAAAGCTAAGTTTTGTTTGGTTATTTACATTCGCAACAACACCATTAGCTACAAAAGTCTTAGTTAAATCATCACCTTTAGCAAAATGACCTATAATTTGTGAATTTGGTTTAAAAATATCACCATCATTTGTACTTATAGAACAATTAATATCAGCTGATGTTGAAGTAATATTTTTAACTTCATGTGAACTAATTGTTGTTGCTGATGGATTTATAACTATTTTTTTATTTGTAATTGATGAATTAATTGGAATTTGATTTGTGTTTGTGCATGCAAAATCATTATTTTGGTCTGAGAAATAAATAGCTTGGATTTCATAAGCCTTATTAGCAATTAATTTGTCATGACCAATCTCCACACTAGCTTTATTATTAGTAATTTTAAAAGCACTACTAAATTGGTCATCTTGTTTAAACTTATGACCACTTACACTATATTTAACACGAATGTACTTATTGTTTAATAAATTAGAACTTAATTCAATCGGTCAACTAATCTTGGTGTTTTGATCATAATCAAGATCTTGTTTATTAATTGTAACTTTATTAACTTCACAATCTAAAGCAATTTTTTGATTTATATCTTGATTATTAGTACTTAAAAGAGCTAAAGGATGTTGGGTATCATTAATTTTAGCAAATGCGGCATGAATTGGTTTATTAGCAAAACTAATTTCAAAAATACCATAATCTTGATTTAAAGTTAAATTATCAATATCAAATTCACAATAGAATTGTTTATTTTGTTGATCATATTTAATTGTAATATTGTTAATTAAATTAGCATTTTGCGTTTGATTAAATAAAGCATATTTAACATTTAATAATTCTTTTGAGCAATCAGTTGAATTTAAAATACCATCAGGATCATTAATTATATATTTAACATGTGCACTAACAGATTGATTATCTTGAACTTTTGTAATTGTAATTGTTGGTTTTTGCACATCCATTTTATTTGGCGCTAACCCTAAAGCTAATGATAGATGATTTTCATTTAAATCTTTTTTAATTTCATTGTTTGCACCCTTGTTTTTAATTGCACAAATTTCACTCAACTCATATTGATGATTAGGTGTAATTTGTGATTTTAACAATTCAAATTCATAAACTTGATTTGAATTTGAATTAATGTTATTAGAGCTTTTAATTTCAATAGCTGGTTTGTTAGGATTTGTTTTATCTACAAATTTAGCATAAATTTCATATCCTTCAAGTTCATGGCCTTCTTTATGTAAACCAACACTAAATTTAACATTTTCGTCTTTTGCACATTCATAAACTCCATTTACTAATTTAGAGTTTGATGCTAATTTAAAATCTTTAATGAAATTATGAGCAACCTTAGTTGTTAAATCTAGTTTTTCTAATTGTTTTTTAGTAGTTTCATTATATTGAATTGGTGATGTTTTTACATTAGGTGAATGAACATGTGTTGTGCTAATAGCACCAATTTCATAAGTTGTATTTGGTGTTAAATCCTTTAATGTAACATTAATTTTATGATTTTTAACACTAACTTCACCAGCTTTTGAACTTGTTCAAGCTTGTTCACCTTTTTTGCGATATGTAATTGTAATTTGGTCATTATCAACTAAATTATGATCTTGATCATCATAATCGAATGATAGATGGATTTCATCATACGTATTTGATCCACTTTGAATAACATTATTAACACTAATAGGTGTAGGTTCGATACTAAATTTAGCATCATCTTTATTAAAATTAGGTGTTAAATCAAGTGTATTAGCGTTTTTAAAATCAGCAGTATCACTAATGATTAACTTAACAAATTCAAAATCACGATTAGCAACTAAGCTAGTTTGATTATTATTAAAACTAAATTCTTTATTAAATGGTTTTGTAGTTGTATCTGAATTCAACAATACAGGATCTGATTCAATTTGTTGGTTATTATTATCTTTATAAACTAATTTCGCATATTTGTTTTCTAAACTAGAACCTGTTTTATTAAAACCAACCATTAACTTAATTGATTCATTCTTGTTAAAGTTTGTTTTTGTTGGTTTAACACTATTTAAAGTTGATTTTTCTAAAGTTTTTAAATCAATATTAGTATTATGATTATTAATTGGATAAACTATATTGTCCTTATTAACGCCATTATAGATAGTATCACTTGGTTTGCTTGTGAACCAAACTTTTTTCACTCGATATTTAGTATTAGGTTTTAAATTAATCAGTTGGGTTTTTAATAATCATTCATTATTTTCTTTTTCTAATGTTAAATCATAATCATGCGTTGTTGAATTAATTATTTGATCATGTGCATCAAGTTCATCAATACTGATTTTAGCGATTTTTAGATGGTTATTCTTATCTTCAAAAATTTGGTCATTTGAATGTAACAATAAACTTAAAGTGATTTTATTTTCATCTGCATTTTGTGCTTTTAAATCAACATACGTACTTGATGGTTTAGTGCTTATTGTTTGTGGGTTTACATTATTTTTAATAACTACCATATTTGCATGATTTTCATCAACATTAACATCTTTTGAATAGTATAAAGCAGCAAAAGTGTATAAACGATTTGAAATAAGTTGGTTTTGGTTTAATTCAAAATTTAAAGCAATATTATTTTTATTATTACCTATATTAGTTGGCGCAGGGATTGTATTAGTTCAAACGACACGATTATCATTACTAATATATTTTGCTTTAAAATGATATCCTTGAATTGTTTCAACTCGTGAATCTAAATTAATACTAATATTTTGTTTATTTGCTACATCAACATCAGTTAAGTTATTACTAAAACTTGCAATAGCAAATTTATTTGTAAATACGTATGTTTGTGTTGTATTAACACTAGCATCATAAACAACATTGTTTGGTTCTTTATAATCATTTTTAAAATTAATTTTATAAACACCATTACCAACATCTTGGTTTTCATCAAAGTAGATTTTATAAACTTTATATTCTTTGTTAACTTTAATGTTATTAAGATTAGCAACAAAGAATTTTTGCCCATCAATAATTTCTACTTTACCTTCATGTTCGCTAATCTCATTTAAATTAGCACCATTATTTTCATTTGGTTGAATATTAAACTTAAAGGTTTGATTTTCTTTTAAAACATCATCAAAATCATTGATAGCAAATTTTAGTTGTACTTTTGCACCATTATCACTAATTTCTTTAATTGGTGCACCAATAATCCCTAATGGTTTAGTTGGTAATAATTTAAAAGTTTTATTAAGATTTTCATATATTCATAGATCAATATAATCACTTTCACTAACGTTGCTTTGATTAAAATCTTTATTTTTAAGATAAACGACACGCTCTAAAGTGTATTCGCGATTACGTAATAAATCTTTAAATTCTCATTCATAATTTGCGTCATTTGCTGATGTAACTTTATGAATAAATGAAATTTGCTTACCATCATTTTTACGTTTGAAAATAAATTTGATATCTTTATTAAATAAAGAAGCGCCAATGTTTTCGATTCGTAAATTTATTTTTAAATCTGCTGTGTTTTTATTTTCATGATAAACAGGATCCATACTTAGACTTGTTATTTTAAAAACAGGGGCAAGAGTATAAAAACCAATTGGTCCTGATGGTAGTTCAAAAATGATAGGAGAAACATTCTTGGTTTTTTGATTCTTAATTGTAACACTCACAATTTCATAATCAAGACCAGCAATTAAATTAGTTGCCATACATTTTGTATATCTTTTATCTTGATCACTAATTGTAGCTGGGGTTGAAATAATATTTTGTGTTCCTTTAATACGATAATTGATATTAATTTCATCACCGCTATGTAGTTGATTATCACTATCATCTAAAATTAATTTAATCAAGGCCTGGTTTTGTCATACATCAATAGCGTTTTTGCTAACTTTAATTTTTGCTGGCGTAGTACTAAAGCTTGAATTAACTAAAGAGGGATTTAAAGCTAAAATATTTTTTTGTTCATTAATAGCATCAAAATTTTGTTCATCACCATAAACAACTTTTAATAAACGATAAAGGTGATTTGGTTTTAAATTACTAAAACTAAAATTTTGTAAACTAATTAAATTAGTTTGGTCTAATTTATTAACTAAAAGATCGTTTGTACTTATTAGTTGGTGTTCATTGTCCTCAAATACTACTTTAACATATTTATTATTAAGCTTATTATTGTTCTTAATAATATTAATATTGAATTTAACATCATATATGCCATTATTATTTTTTGGATAAGCATCTGCTAAATTACTAATTTCATTAACAATTGATTTTGATAATGTATGAATAGTTTTTTGATTATCTTCAATATTGTTTAAAAGAATTTCATTATTATTGTTGTTATTAATATTAACATTAGCTAATTGTGGTTTTTGTTTTAAACTAACACTTGTTAAACGATAAGTATGATCTGCTAATAAATTAGTAATTACCCCTGAAGCACTAAAACTATGTTCATCTTTTTTAACTAATGTTGTTTGTGTTTTTAAAACTGGTTGGTTTTTATCTACTAAATTTTCAAAACATAATTCAACAAGCATTCCAGGTTCTAAAACTTGATCATCATTATTAATGTTAAAATCATAATTAAATTGATCTTCATCAACATTGTGTGTTTTAAATTCTAATAGTTGGGTAGTTGAAGCTAAAGTACTAAAATCATAAGTTTTTGAATGCTCTTTTACAAAAATCTTCTTACCTTGGGTTTCATCATTATTTTGATTTTGATCATTAAAATAATAAATTCCTGCAAAATTGTATTTACGATTAGGTGTTAAATGATTGAAATTTAAATTAATATGTGCTAAATCAACATTATTTACACTAGCATAATCACTTCAAATTTTTTGGTGGTTATTATCAATATATAAAGCTCGTAAATATTGGTCTTTTAATAAGTTTTGACTAGATTTTAATTCAACATCAATGCTTGCAGTAGGATTTAAATTTGTATTAGGTTGATTAACATTAATGTTGTTAACAAATAATTCGTTTGTAAAACTTAGAATTTTACTTGGATTTGAACTAGCATCATAAATACTATTTTTATTAGAATTATTACTAATAGCATTGGCAGGTTGAATTTTATTTTTTGTTGCATAATTAATATTATCAACAATGTATTTATGATTTGGTTGAATTTTAAATGTGTTAGTGTTTTCTACACTAAATTCAAGTGTTTTATTATTATTAGCGTCAACATTGATTTTACCAATAACATTATGAGTTGTTTTATCTTTTTCATCATGGAATGTAATTTCTAAAATACTATCTTGTTCTAAAAAATCATTAGGATCATTTATTTGAAGTTTTAAATTAATTTTATTTAAATTTGAATCTGGTTTGCGAACTAGTTTAGTATCTATTGTTAATTGCTCGTTAACTGATGCTGTTTTAAAAGTGTTTGTAATTTTTTGATTATTTAAAAAACTAAATTTATAATCATTTTGATTAGGTTCAGTATAAATCAGTTTTTTAAATGAATAAATACGATTATGTTTTAAATCATCTAAATTAAAATCATATTCATTAACACCATAATTTAAAACAACAAAGTTATTAGATACTAAATCATGTTCCCCATCGTTATACACAATTTTAGCTTTACAATTATTTAAACTTGTTCCATCATTATTAATAACAATATGAACACTATTAGTTTTTACATTCGCATCTACTACATCACTTGCAGTAACACTAACTAAATCAAAACTTGCTTTTGGAGTACTAAAACCTGGTGTTTTTTGTGAATCAATTAATACATGATTATCATCACTATTATTAACATTAAAAGCACCTAATACTGGTTTTTGTCTAAATTTAACAGATTTGATCTGATAATTAACTTCTGGGCTTAAATTATTTGCATGAATTTTAACGCCTCACGTTTTTGTTATATCATCATATTCTAAATTAGATGTAAACATTAAATCATTTTTCGTATTATCTTTATTGCCAATAGTAACATCAATAACTTGATCTTTTGCAAAAATATCATCGGTTGACACTAATGGTAAATATAAATCCGCACTAGCAGGAGTGATGTTATTAATAGTTACTTTTGGTGAATTAAGATTGATTTTTGAAGCTGTTTGAATTTGTAAAGGTGTTATATTGTTCATATTAACCTTATTAGCTGATGTTATCTTAACAGTATTAACATCTTTGAAATAATATAACCCATCAAATTTATAAATACGGTTTGATTTTAATGCATTTTTGTCTGGTAAATTAAATTCATATTCATTATTAACATTATTAATTGAAACAGGATCAGATCAAACAACATCACCATTATTATCAATATACTTTAGTTTTAAATAGTATTCATTCATTAAATTCAATGAACGTTTAAGGGTTAATTTAACTTTATAATTTAAAACATTTGTTTTTACATCTGTTATATTTGGCTCATAATTATAAGTATTAACTAATAATTCATTACCAAATTCACTCAATTGTGGTTGAGAATTATTTAACTCATAAACTTGTTCATGATTTTCATTTTGATTAAAAGCAGCGTTGATTGGTTTATTAATCAACGTGATATTGTTAATATGATATTTATGGTTTAATGAAACATTAGGAACATCAAACTCTAAATTACGATTCCCGTTTTGATCAATCACTATTTTTTGATTATCTGCATCAAAAAGATTATTAGGATCATCTAAATCACTAATTTTAATAGTAAATGAACTACCATCACTGTCAACTAAGACATGATCATTATCAACTAATGGAATTGATAATCTAATACTATCATCAGTACGTTTAATTACTTGTAAACTATTATTTTTTAAACTAATAATTCCTGTTGGTTCTGTTTTAAAACCATGACTTAAATCAGTGCTATTTTTTATCACCGCATCAGTGCTTTGTAAATCATTTTGATCTTGTGCATATATTAATTTAACAAATTTATAAACACGATTTGATAAAAAACCATTTGGTTTATTATTTGTTAATGTGAATTTATAGTTACTATTGCCAACATTTAATAATAAAGGTTGAGATAAACAAACTTCATTATCTTTACTAATATAAACTAATTGGGCTCAAACGTTATTTAATGTTTTATCTTTTTGTGAGAAAACAATTTTTACATTAACATCAAGTTTATTTTCATCTTTTTTAATTACTTCATTTGAACTAATTGATTCAATATATGTTCCAATTAATTTCGTACTAAATTGATTATGTTTTTGATCAAAAGGGGCAAATACAACTTTTTGATACGCATCATTTGCTAAACCTAAGGCCTTTAATTCTAACAATTCATAATTATTATTAGATACTAAATTATTAAGTTCAAATTCTAACGTACCACTTTTGTTGGTTTTGTTAATTCCTTTAACATACCCTATTGTTTTATAAACTTGTTGATTATTCTTATTTAAATAAGTTAATTCAACTGGGTTACCAATTCTTAATTTTTGATCTTGATCTACAAAATCAATTGCTATTCGTGCATCTTGTGATTTAATAATTTGTTTAGGATTGTTAACATCAAAATTAACTATAGTAGCATGTGGAGTTGTACCAAAAATTAATGGAGTTTTAATTGGAGTTGAATAATGAATTTTATTGTTGTTTTCATCATTAAAACCCAACAATAAATATTTACTATTGCCTTTTAAATTTTGCAAATGTGCTACATAATTTTTTTTCACTAAATCATAATTTAGGTTAGCAACTAAATCTGGTTCTAATGCGTTATTATTTTCATCTAATAAACGATAAGTAATTGTTTTGGGGTGCTTATTAGTTGTACTAATATTTAAATTTAATTTAGTTGCATTAGGATCAGTATCATATTGATGATAATCCTCTAATACCAATGTATTAGTAGAAAAAATTCTAAATGTATAACCAATAACTTGATCTTTAATAATATTGATTTTAGTTTGACTATGAGCTTCAGCGTTTAAATCAGTAAGATTTAAATTATTATTTTTAAAAACTAAATTTTTAATGTAATAAGCAGTATCTGTTTGTAAATGATTAAAATCAAAAACTAAACTATTATTTTTATAATCAATTAAGCCATAAGCACTAACACAATGTTTTGGATCGTTTTTTAAAGCAAGTGTTAATTTTAATCGTTCATTTAAAGCTAATTTATTAGGGTCATTAACTGTTAAAATTAAAGTTGTGGGTTTGTTTTGAATAACACTAGGATTTTTAACAATAATTTTACGGCCCAGATCAACTTCATTATTTAATGTTTTAACTGAATTATCAACATCATCTGAACTAATAATTGTTTCTTTAGGAATGTGATATTCATCTTCTATGCTAATTGAATCAAAATGATAAATCTTATCTTTTGTAAAATTAGGGTTGTTTGATAGATCAATTTGTAGTTGGTGTTTATTATTAACAATTGCAGGAATTTTTATCAAATTACCATCAACATCTTGGTAATTAACAAAAACGTCTTCGCCTTCTTCAACATTTTTTGGCACATCAAAAACTAATTGGTTATTAACAATTTTACCAATATTTAGTTTGTTAACAATAATTTGTTTTTGATCCAAATCAGCAACATTAATTACTTTTTTGACCATCATATTTAAGAGCAATTAGTTGATATTTATGATGGTTAGGTAAAACCTCATCACTAGTTATAAATCGTGTTTGACCTAATGAATCAATAATAGTTTTTGCTTTAACATGATATTCTTTTTGATTTTCATCAATAAAAGTTGCATCTAATGAACGATTAAACAAATTCTTATCAAAAGTTAGTAGGTTTAAATTTTTATTTGCATCTTGTTTAATAATTGCAAGTGGTTTAATAATGCTAATTCTGTTTAAAGGATTAATTTCGTTTGAATTTAATAAAATTTGCTCATGCTTGTTTTTATCAACAATATTTAACAATTGATAGTGGTGGTTTGCATCTAAATCTTTTGTGTTAATACTTATTTTATTCTTATTTAAATCAACTTTAGTAGGAACAAAATAATCTTTATTATTTTCATCAATAAAATGAGCAACAGCATCTTGAGTTGTTATTTTATTTAAATTATCATTAATGTCAATAATTAAATTTCCTAAATCATCATAGGTGTATGTATTTAATTGTTGTAATTTATTTACATCGACTTTTTCTAATAATCTATCTTTTCTATCTAAATCATTAACATTAACGATTGGTTGATTATTAACATTAGTAATTTTAGTCAATGTATAAGTGTGATTATTAGGTAGTAAATGATCATCATTTGTTCTTAAAATTAATTCTTGTTGATCATTTAATTTACCAGTTAATTGATAAGGATGATGATTTTCATCTTCAAAAGTTAAGACAACAGACTTATTAGCTAAATCAGGGTTTAAAAGTTGAATTTTATAATTATTATCTTCTAATGCCCCAACAATCGCAGCTGGTTTACTAAACTTTGTTTTTTGATTAATTGCAAGTTCATAATCATTTAAAACATTTGTTTCTGGCTCTTCTAAACTAATAACATGTTTTAGTTTATATGTATGATTTAAGTTTAAATCAGAAGTGTTAATTACAATATTGCCATTTAGATCAACCTTTGTCAACACTTTATGTTCATTATTTTTTTCATCAATAAACACGGCTTCAACTTGATCATTAGCGAATTGTTTAGAATCTACTTTAGCATTAAAAATTAAATTCCCTTTATCATCATAATGATATGGATCATTTAATGCATTAGTGTTTAATTTATTAATAGTTTTAGCACTTTCATTTAAATCATCTAAGTTAGCAACGCGTTTTGCTGGAGAGGTATTATTAATGCTAATATTATCTAGTTTATATAAGTGTCCATCTTTTAAATTGTTTAAATCTACAGTAATTTCACCATTATTAACCTTAGCAATAATATTGGTTTTATCGTGGTTTTCGTTTTCAAAACTAAGTGTTAATAATCGATCATTTAAACTTGAATCAACTTGAATATGAAGTGCTTTTTGTAAACCATTTTTATCAGTTTCAATTTTTATTTTATTTGGTTTATGGACAATATTTTGATGTGCCGCATCTAATTTATTAGGTTTTATAACATCATGATTATCCTTATCAACAATTTGAATTAGTTTATAATCATGGTGATTTTCTAAATCTTTTGTTAATAAACGCACAAGACCATCAGTACCAACTATTGCCTTTAAAGTGTGTAATTGGTGTTTTTCATCTTCAAATAAGGCATTAATATTTTGATTAGCTAAATTTTGTGAAACACGTGCGTTAATAATAACGTTTCCTTGATCATCAAAACTGCGTGTTTTATCAATACCACGAATGGGTTCACTAATTTCATCTAAATTAACAATTTTTGTGTTTTTATTATCTTCTTTTAAAGTTAAACCTACGAATTTATAAAAATTTCCTAAAGGTAGTTGTTTAGTTCCAACAATTACATTACCATTATGATCAACCTGAATATTTTGAATATATTTAGCATTATTTTGATTAATAAAATGCGCAGTCAAATACTGATTACTCAATTCATGGTTTAATTTAATTTCAAAAAATTTATCGCCTTCTTTATTAAAATTAACTTTAAAGACATTAGGACGATCGACAAGTATTTTTTGTTGCCATACCAACTCATTATTATTGATTAATGTATTATTATTTTCATCAACAATACGATCTAAAACTCAAGAATTATTCAAACCTAAACTCGTTGTGTCAATTCGTGCTACTTCTTTATTTCTTGTAATTAAAACTGAAGTTTCCTTGCCTAATTTATCTTTAAAAATCGCATAAATCTTTTGGTTAATATCATTATCTCTTGTAAATAAACGAACCTTAATAATTAAATTACCATCTTTTGTATATTCATAGTATTTACTAAAAATTTGATTGGAATGCTGATTTGCGAAACGTAATACAATTCCTGTAATTACACCAGCTCAAATTAAAGAACTAATTGTAAGTCCAGTAGCTATTTTGGCATTACGTTTTTTATTTTTATTATTCATAAATAATCATCTCTTTTTTATAAAATTTTCCTGTTTTTCAGTTTAAAAAAAAAAAAAAAAAACTAATTTATTTTGTGTAAACTGTTAAACTACACAAATTATACCATTTATCCGCTTGCTCTCTTGTTACAAAATACACAAATAATGTAAGTTTTTACTATATTGAAAATATCTTTGGTTATAATTTAATTTGATAATAAATTTAAATTTTATGATAGGTTAAAAATATGAAAAAAAACACACATAAGAAACTTTTGCCTTTAATTTCTATTTTTGTTTTAATCCCTTTGAGTTCACTTGTTGTAATGTGTTCAAAAACTAAAACAAAAATAGTAAAAGAACAAAAGCAAATTACTGCTTCATCGTTAAGATCTACTGATATTGATTTAACATCAATTAACCTTGAAATCTATTTTGCTAAATTTGATGTTAATGATATTTTAGTTAAAAAATTCAGCATTGAATTAGAAGATGATAAGGGAAATAAGATAAATGTTGATATAAATCCCGAATATAACAAAAATTTACAAATGCTAAGTTTTAAATTAGAAAACTTAAAACCAAACACTACATATAAAATTACAAAGTTTAATATCACAGGACATGAGGTTGATCTAACTCAAATAAAAGATTCATTATTCACTACTAAAGCTGAGAATCTTTTACCTGATATCCCTAATTTACCTAATAGTGTTGGTATTGAAATTAAAGATATTAAAACTAATTCACTTAGCAATGATGCAACAAAAGTGAATGTTAATGTCAATTTAGAAATTAACCAATCAACATTAGAAAATCAATATGCCCGATTAGTTTATAAAAGCAATGATAATTCTTGAAAGCTATCAAATACTTTAAAAATTGCTGAAATTAAAAATAGTAATTTTGTTTTAGATGGTTTAATATCAAACCGTAAATATCTTTTTAAAGAATTAATTATTGGAAGCCAAAGTGATTTAAATTTAACTAATGCGCAAACTAAAATAACTACTAATAATGAGCTAAGTTTCACTACAGCTCCAAAACCTGTTGAAATAAAAAGTGTTTTAATTGATACAAAATTTGATAATCATCCAAGTAGTTTAATTAATTTGAAGTTTAATGATAGTGAGAATAATTTAAAAGAAAATGATATTTTAAAAATTAAATATAAAAAAGTAGGACCAAATGAAGTCATCTTTGAAAAAACAGTTCGATTAGCTAATAATTTTGAACTATTATTTGAAATTGAAAATACAAAAAAGAATGAAAAATATGAAATCTTAAGTTTAGAATCAAACACTAAACATGGATATAATGTTGCACCAAGTATATTTAATTTTATTTCTAATGATTTACGCACTTTTGAAATCAAAGGTTAATTTGAATAAAAATCTAGTTACTCATTAAAAAAACGGCACGGCGCCGTTTTTTTTTTTTTTTTTTTGATTGGTTAATTGCTTATCTTGTAATATAAGTAATTACATTAATCGGTTGTATCATTCAACAACGTAAGCTTCATTTACATCTGCTGGTAATTCATTACGTTCTGGTAAACGTGCTAATTTTGCAACTTTTTTTTCTTTATCGATTAATTCAATAAAATCAGCTGGTTTGTTTAAAGTATTTTTAATTAAAGGTATTTCTAATGATTTAGCCTTAAGAGCAATTGTACTACCTACTTCAACTAGTGCTGAAGGGATGTTAACCTTTTTATTGTTAACCAAAACGTGTGAGTGATTTACTAATTGACGTGCAGCACGACGTGTTGGAGCAAATCCAGCACGATAAACTAATGAATCTAAACGTGATTCAAGAACTTGTAATAAATTTAAAGTAAGTACGCCTGGACGGCCTAGTGCAACTCTATATAAACGTCTAAATTGACGATCATTCAATCCATATAAAAACATTAACTTTTGTTTTTCAACTAATTGTTGGCCATAATTTGATAATTTAACTTTTTTATTACCGTGTTGTCCTGGACCGTATGTTCTTTTTTTACCAGAATTAAATTCTTTATTATTTTCTAATAAAGAAAATCCTAAACGGCGTGATTTTTTATAAATACTACCTGTATAACGACTCATGATTATATTCTCCTCAAATTTATTTTAAGCATATAATCACAAAAATTTAGATTCTATAATTTTTTATTTAATTTTGATAGTTTCAGAGAAGTCAGCAACTTTCTTACTAATAAACATATTTTTTTAATATGAATTAAATAGGCAAATGATATATCTAATAAGCTGATTAATATGCTATTAACTATTATAATATATTTTGTGATTTAAAAAATACAAAATTATTTGATTATAATTTAATATACACCATCTATAAAGGAATTTGTTAATGTCTTTAATTTATCTTCTAATAGGTTTTATTTTATTATTTTTACTTATTTACTCTACTTTTGCATTATTGGACATTTGAACACTTAGAAAATATAACACATTCCGTCAAAATTTTTTTAATTTTGATCAATTAGTCAATACAAAATTTAATGATATTTATACTCATACAGATAATATCTATGCACGTACAAGTAAACAAAAAATAACAATTATTAATAAATACCTAATTGCAATTAATAATGAATTAGATTTTCATAAAAAAAGTCTAATTAAATTACAAGATTATCTTGAAAATCATAAATTATTCTCTTATTTATCACTTATTAAGTTTATTAAAGCAAATGAAATTAAAATTAAAAATAATGTAGATGAATTTGTAGATTTAATTCAATCTTTTTGAAATGATTTTATTATTATTGATCAACAAGAAAGAAAAATTGAAGATACCCATAAGAAATTAGATTTAATGTATAAAGATCTTATAAGTAAATATAGTATTCCAAATTTAAATTATCAAAAAGCAATTAATTGAACTACTGATTCATTTAATAATTTAATTGAAACTAAAATTAGTATGAATTATTCAAAGTATAATTATTTTTTAGTTCAATTAATTCATCGTATTAAAAATAAAGTTCATTTATTAAAAAATATCATTGTAGTTTTAAATTCAATCAAAATTATTGAAGAAGCTAATAAAAATAGCTTACAAAAAGTGCAAGAAATCACCTTTTTGACTGATGACAAAATTAAAGAATTAAGACAATTTAACCATGATTTTAATGATTTTATTAATAACCAATTAGCTGATTTTATTTATAATTCAAAATTTGTTAATTTAAATAACCTTTATTTAAATAGTTATTATCAACAAGCCTTAAATTGACTTAATATTAGTTCGCAAAACAATATTGGTGGGGCTGTTTTTGATAAATATAAAAAACGTTTTTATGATGATTTTAGCTTCATTAATAGCACTATTATTAAAGTAAATAATATTTTAGAAAAAATAAAGAGCACTTCAAAAAACTATCAATTAAACCAATTAAACCAATTAATTTTAGACTTAGATCAAGCTAATCAAAAAATTATTAATTCTAAAATTGATAAAAATGATATCAAACAAAATATTGATTTCATTATTTCAACACAATTATTCTATGATAATTTAAAAAATTTATTGTTTTATTTAAATGATTTAAATGTTAAGTTAGCAAATTGGAATCACCACTGAAATACACTAGTTAAAAAAACTATCACTATTTATAATAAAAAAATCGAACTTGAATCTTATATTAAAAACAAAGATATATTTTTTAATTTATCTAATGAAATTTTTTTAGATGATTTCGAATATATTGAAAATATATTTCAAAAAATAAATGCTTCAAATAGTTTTGATCCCCAAATTTTAACCAATCATGAGTTAATTATTCACCAATTAAATTTAATTAATGAACGTTTTGATTATCTTATTGAATATATTAACCAAACCCAATTATGGATGCCAAAAATCTATGATTTGCTTTATAATAAAATAAATTATTATGGTATCAACTATGAATGAATAAAAAAAGAATTTCAAAATTTAAATTGAAACAATGTGCCTCAAGCATCTAAATTATTAAATGAATTTATTGATAATATTATTATTGCTGATCACGCTTTAAAACGTTTAAAATTTAATTAATAACAAAAAATACTAGCTTTAAGCTAGTATTTTTAATTTTATTTAAATGGTGGTTTCGGACAGAATCGAACTGTCGACACACGGATCTTCAGTCCGTTGCTCTACCGACTGAGCTACGAAACCATGGCGGTCCTGACGGGAGTCAAACCCGCGATCTCCTCTGTGACAGAGAGGCATGTTAATCACTACACCACAGAACCATTGGTTGCGGGAGAGGGATTTGAACCCCCGACCTCCAGATTATGAGCCTGACGAGCTACCAAGCTGCTCTATCCCGCGATAATAACTTTATAATGTTGTGTGTAAACATTGATAAAGTATTTTATATTTATATAATGGCGGAGAAAGAGGGATTCGAACCCCCGCGCGACTTGCATCGCCTCTCAGTTTTCAAGACTGATCCCTTCAGCCGGACTTGGGTATTTCTCCACAGTATATACTAAACTACAACCTTTTTATTATAACATAGTTTAGTACATTAATTTTATATATTTTATTGGTGCTTCTAACAGGACTTGAACCTGTAACCAACCGATTATGAGTCGGGGGCTCTAACCAAATTGAGCTATAGAAGCAATGGTGGCTGGACAGAGATTTGAACTCTGAACCTCCCGGGTATGAACCGAGCGCTCTAACCAGTTGAGCTATCCAGCCAAAATGGTCGGGAAGACAGGATTCGAACCTGCGACCCCTTGGTCCCAAACCAAGTGCACTACCAAGCTGTGCTACTTCCCGAACGATTAAGATAATATTTCATAATACTATCTTTTTAAAAAATTTAATGGCGCACCCTAAAGGAGTCGAACCCTTAACCTTCTGGTCCGTAGCCAGACGCTCTGTCCAATTGAGCTAAGGATGCATATGGAGGTGCCAGTCAGATTCGAACTGACGAATAAGGAGGTTGCAGCTCCTGACCTTACCACTTGGCTATGGCACCAAGAATAAAATTAATTCTTTGGAACTAAACCTAATAAATTTTAAATTAATTTTGTGTTTTGAAATATTTAAAAACACATATAAGATAATAACAAAATTAAACTTAAAAGTCAATCTTTTTTTATTTTTCATAAATTACAATAGGTGTAATATTAGTTGGAAAATTTGTTTCATCATCAATTTCAACTAAACTAGCACAAAAAACATATTTTTCATCACTTGGTTCTAATATAAAACGTTTATTTGGATCACGAAATCGTTGAATAATACTACTTGGATTTGCCCCAATCACGCTATTAGCAGCACCACACATTCCTAAATCAGTTTGAAAAGCCGTATGATCGACAATTCTAGCATCATTTGTTGGAATATGTGTATGAGTACCATAAACTAAAGACAATTTACTTTTAAAATCTAAAAAAAGTGCATTTTTTTCACTTGTTGTTTCAGCATGAAAATCAACAATATGAATCGGTGCTTCATTAAAAGCAATAATTTTATCTAAAGATTCAAAAGGATTTGTTTGTAAACCTTTCATATCAATAGAATTACCTAATAAATTAGTTACACGAATCATTTTTTTATTACAATAAAAAACTCGTGAACCAGAACCTACTAAGTGATACTGATGCGTATTAATAATATTATATGGACGAATAATATTTGTTTTGGTTGTTAGGATTTCAAAAATATCAGGATGATCTCAAGTATGATTGCCCATAGTAATACAATCAATTCCATAACCTAATAATTCTTCATAATGTTTTCAACACAAACCGCGACCATGTGTTGTATTCTCAGCATTAGCAATTACAAAATCAATTTGATACTCTTGTTTTAGCTTGGGCAATTCATTGGCCACTGCTTTTCTTCCTTGTTTTGAAAAAATATCTCCAATAGCTAAAATTTTCATTTTTTAACCTATTCTTCTTACGATTGTTTTATGATTAAAATTAATTTGTTTTGGTTGACGATATTTACTGAAAGCAATTGTTAAGATATCACCATCAATTCCAATAATAACACCATCACCAAAACTTGTGTGAACAATTCTTTCACCAATTTCAAAATCGTTTTTCATTGTTTGATAAAAATTATCTTCAACTTCTTTTTGTTTTTGTTTTGAATCGAACCATGAACGTTCTTCTTGTTGTTTTAAGAATGATTTTAATGGCATATCCTCTGGTTTATTATTTTTAAAACTACTATTTATAATCTTTAAATTATTAAAACCAATTTCATTAATAAAACGACTTTCAAATACTTGTCTTCTTGCTAATGGATCATAACAACAAGCAGATGAAATATAAAGATTTTTTTTTGCTCGTGTTAAGGCTACATAAGCAATTCTTCGTTCTTCTTCAACTCCTTTAATTGGATCATCATTAATTGCTCGATCAGAAGGAAGAAGTCCTTCGTTCATACCAGCAACGAAAACGTTTTCATATTCTAGACCTTTAGAATTATGAACTGTCATTAATAAAACATTTTCTTTACTATCTTTACCATCCTTATTCAAATATAAAGCAATCGAATTTAAATAATCATTAATCGTGTCATCTGGATGACGATCAACATACATTTTCATAGCGCGTTTTAATTCATAAACATTATCGATTTTATCTTCTGCTTTTTCGTTTTCTAATAAATAAGCTTCATAGTTAACTTTTTTGTAAATTAATTCTAAAATCTCATGAATTTTTAAATCTTCCATTTCATCACGGATTTCTTTAATTAAATTAACAAAATTTAAAATATTCTTTTTTTGTGTGTTATTTATTAAATGGTTTTTTTCAACTTCAGCTAATGCTTCAAATAAAAATAAATTATTATTTATTGCATAATGTTTAACATGTTCAAAAGTAGTTGGACCAATCGCACGACGTGGTGTATTAATAATCCTTAAAAAAGATAGTTCATCTAAATCATTAACTACTTTTAAATAAGCAATCATGTCCTTAATTTCAGCACGTTCATAAAACTTTTTACTGCCAAGAATTGTATAAGGAATGCTCTCGCGCATCATTGATTGTTCAATCGTACGAGAATAGTGATTGCTTCGGAACAAAACAGCGATTTGGTTAGCTGGCGTTCCTTCTTCTAACAATTCTCTAATTTTTCTAGCAATTCAATTAGCTTCATCAATTGGGTTTTGCCCTCGTAAATAAATTGGTAAATTATTGTCATTAGTATTAAAAGCAGTTAAATTTTTTTTATAATTGTTGTTATTAGCATCGATTAATCTATTAGCTGTATTTAAAATTGGCTGAGTTGAACGATAATTTGTTTTTAAAATTGTTACTTTTGTATTCTTAAAATTTTGACTAAAATTATTAATAATTCATTGTTCAGCACCTCTAAATGAATAAATCATTTGATCAGGGTCGCCAACCACAAATAAATTTTGATGTTTAGTTGCTAATAAACTAATTAAATCATATTGAATTTTATTAGTATCTTGAAATTCATCAACAAGAATATAATCAAATTTATTTTGTCATTTTTCTAAAACTTCTGGAAACTCAATAAATAACTTATGAGTTAAATTGATCAAATCATCAAAATCAACATAATTTAATTTAAAACATCGATCACAATAAATCTTATAAATATCAACTAATCTTTGTGCATCATTTAAATCAACCAATTCTAAATACTTGTGATTTTTTTCATTTAGCATATCGTTTTGATCAAAAAATTTATTTTTAACTTTACTAATCGTTTTAATTATTTTCTTAATAACTTGCGATTTTTTTTCAATGTTTAATTGATCAAAAATTTCTTTAAGCAAAACTTCTTGATCGTCTGTATCAATAATTTTAATATTGTTATGATAATTTAAATAATGAATATCTTGTTGTAAAATTTTTACACACATTGAATGAAAAGTACTTAAATAAGGAATAGAAACGCCAATTGTTTTTCCAACACGTTCTTTCATTTCATCAGCAGCCTTATTAGTAAAAGTAAAACCTAAAATCCGTGATGGATGAATATTTTTTTCAGTAATTAAATAAGCAATACGTAAAGTTAAAACAGATGTTTTTCCTGTCCCTGCCCCTGCAATTACTAATTGCGGTCCTAAGTCTGCTGTAACTGCTTCTTTTTGTTCTTGATTTAAGCGTGAATAATCAATTCCTAATCCCATAAAAGCTTCTCCTTATTTTTCATTTTATATTTTATCTAGTTCTTGAATTAAATTATCGATTTTTTGAATCTCAACTTCACGTTTTGTTTTATGTTTAGGCGCTTGTTTTGGTTTCTCATAATGCATCCATAATAAAACAACTTTTGCTACTTGATCATGTAAAGCACGTTTTTTTGAATTCATAAACATGTGAATTACAATAATACAACTAACTAAACCCATAATTGAATATAGACTTTTAACTAAAATACTTAAAATAGCCATAGTTGTAGTATTGGTGAAATTATCATTAATTTTTAAAGATAAAATTGACTGCACATAAGCTTTTAAATCTTTAATTTGATGATAATACTCTTTTAGCTCAGTCTCATTCATAGCTTGAATTGATTTAAAACTTGCATCAGCAAAAACAAAACAAAATCCCATCGCTAATAAATTAATTATGGTAAAAATTAATCAAGTTAATAAACTATGTTTAATTAAAGCAATAAATAAAATTTTTTTATCGTTTTGTTTTACTAAACTAATCATTCTAATCGAAAATGCTCATCGAAATAAAGTATAAGATTTACAAAATAAAGGAATAATAATGTAGTATGTAGAATAAACAAAAAAAGCAATTATTCCCATTAAGAATAAACGTCAAGGTTCAGGAGCAAGCTCATCATTATTAACAAAAATATCACTAATTTTAAAATTACTATTAATAAAAACTAATAAAGATAATAAAAAAGCAATAATGGAAATAAAAATATAATCTAAAACACTAGCGCTAGCACGCCTTAGTGCACCAACAACGGGATATTCGATCGTATTTTCTTGTTTTAATGTTGACTTATCACTCATTTAATTTAATCCCTGTTTTGATTTTAAATAATATTTTAAAATATTAATTTTAAACTTGCGAACATATAAATTATTCTCAAAATTAGCAAAATAAATCTCTAAATACTTTTTAGCATTTGATAAGGCATGACTAATAATCATTGTTCGTTCTTTAATTTGTTTTTTAGAATTAATATTAATTGTGTATAATTCATAAATTTTTTTCAAAAAAGAATGAAAACAAATTCCCGTAATTCAATAACACAATTCGTCCTTATAAAGATCTAATAAATCATCATTACTAAATCTTATGTATAATTCATTAATTTGTTGTAGTAAATCATAAACATTATAACCAACCTCACCACTATTATTGCTAATGAAGTTAATATTTACATTTCTTCATGAATTAAATTTGGTAAAAAGATCATAAATAAATAAAGCAGGGTATCATTTATCATTATAAAAAGCTAGTTTGTTTTTTCTAATAAAATCTAAAGCAATAATTTTATTAGAAACTGATTCTCATAATCCAACTAATATCTCTTTATTCAAACTATCAAAAACCAATGATGGATTTTGGTTAACATTATCATTTAAATTAAAACTAATAACATCGTAATCTTTCTCTAAAACCGAAAACAACAATGAACTAGCATCTAAATTTAATTCAACATGACCTCCTAAATATCAAATGTATTTACCGTGAGCATATTTCGTTGCTAAATTATATGCATAAGCATTGCCTTGTGTTTCATTTAAATAAAAATATTTAATCTTAGCATTAGTATTTTCAATTAATGGTTTTAATGTTTTAAATAAATTATAATCACTAGCATCATCAATAATAATTAATTCAATATTTTTTTGATTTTGATTTAATAAATCTAAAAAATAATTAAAATTTTTTTTTAGATAATGAGTTGTCTTTGATAAATAAAAAGCAATTGTTAATAACATGATTTGACCTCATATCTTATTATTATACTATTATATAAAAATAAAACTAGAAGCAAAATAAAATGCTCTAGTTTTATTTTAATTAATGGCGCACCCAAAGAGACTCGAACTCCTAACCCTATGCTTCGAAGGCATATATTCTAACCAATTGAACTATGGGTGCATTATTTTATATATTTTATATTATTTTAATAACAAAAAAAAAAAAAATCTGCAACCATGCAGATTTTAGTGTATCAATGGCGCACCTGAAGGGATTCGAACCCCAGACCGTACGCTTAGAAGGCGTATGCTCTATCCAACTGAGCTACAGGTGCAAATACAATTTATTATAACTTAAATCTTATTAAATTAATTAATTTAATTTTCATTTTTAATATTTTAATACTAATTATTTAGTGGAGGTATTAATTTATGAACACAGAAAAAATAATACAAATTTTTTTATATAAAACAAAATTATTCCGTATTCAATATTTATTTTTAGATGTTTGAGATGAAAAACAATACTTAAAAACTGAGGTAATTAAAGATTTGCAATTTATTAATAAATGAAACATTAATAAAATTAAAAACTTACATTCTAATGAAAAAGAAGGTGATAAAAATGATCAATAATTCACCAAACATTAATTATTATTTACAAGATAAAAAATATATTCCAAAATATGCTTACTATGTTAATAAAAAACAAAAACCTATCGAGCTATTTGCACGATATAAAGGACGTCCTGCTCTTTTTATTCGTGAAAATACCATGATTAATACTTCATTAAATTCAAATGTATTAAATTTAGACTTTGAATTTGAAGTTGCAGGAGATTTTAAATTACCTATTAATTACTTTTTTATGGATATAAAAAGTGATATAGGTGAATTTCGTAATTTAGAAGTTTATGAAACTAAACACTCTTTGTATGAAATCGGAGGAGTGATTTATGACTTATACACTTATAAAACCAATTTAAAAATTAATCTTAATCAAGCTGGTTTTGTTAATAAGAACACTTTAAGTAAAAAAATTAATTTTGCTTTTTTTCTTCGTATTAATGATGATAAACGAATTCATGAACAAATTAATTATACATGTGAAAAAAGTTTGTTTTTTGAGGCAGTACAAAAAGAATTTCAAGATCTTAATTTTGATTATTTTTACAAAAATACATTTTTATTAAATAAACACGAAAAAGTTAATCCTACTAATTTTTGAGAAACAATTCCATTATTTTGAATTCAAGATAAAGAACTTGATAATGAAATAAAAATCAAAAATAATAAGCGTTATATATTTAATACAAAATTTAGTTTAAATGATGATAATTCATGAAATAATCGTCCTACGAAAGAAACGTTTGAATTGATTAAATACTCCTTAGAATACGTAAATTTAGCTAAAGAAAATAAAAAAATTGAATGAATTGAAGATTATCAAAATATTAATATTACATATGATTATTACCAAAGACCTATTGATTTTTTATTAAAAATGAAAACAAAATTCTTGTATGATTTTAAAAACGATCAATTATCTATTAATAATAATTATGGTTTTAATGGTATTTGATTACCAAATAAACAAATAGCAAAATTAAAAATTACAGTTATTAATAATTATCTAACACCACAAAAATTTGTAATTAATCAATTAATTAACATCGAAAATGATTTTAATTCTATCAATAAACAAAAAATTAGTGTACAGAAAGCAATAATTGAAAATTATGAAAATTTTAAATCACTTGAAAATTAATTGGCGTAGTCATAAAATAAAGGTGATTGTTGGTGGAATTATTAGTTTATTAATTGTAATTACTGCTATTATTTTAGGTGTTATTCTATCAACTCAAAATACAAATAAAATTAGTAAAAAAGAACCCTTAACTCAAATTGAAGATAATATTAATAATAAAAAACAAGATGCACATAAGGTTAATGAAAATATAACAATTATTGATAAAAAAGGTGGTATAATCCATAAAAAGACAGACATTACAAAAATTAACCAAATAAATCAACAAAAAGAAATTTTAAAGGATTATTTAAAAAATAAAGAAGAAAAAAATAAGGATCAAATAAATAATACTAACGAAAATCTAAATAAACCAATAATTAATGTTAAAAATGTAGATGATAAAAACAAACAAGAAAATTCAACTAAATTAAAAAATAATGATTTTATTTCTAATAATGATAAAAACAATAAAATCAACGAAAATAATAATATAAGTTATGAAGAAAAACCTTTTAAACTAAAACGCTTCAATATTATTAATGTAATTGCGAAAAATAAAGATTTATACCAATTATTAGATTTCAAAACTTATTTTTCTAATGAAAACATTAATAATGATATTAAATTTAATGAAAAGTTGTTTATTAAAAATATCTATGAAATAGTCAAATCTGCTATAAGTAGTTTTCAAGAATTTTGTAATATCATGCAGTATATTAAAATTGATATCAAATATAAATTTAATAAAGATGCAAAAAACATAATTGTTATTGCTAATTGATTATTCGATAATTATAATATTAAAAATGAAACAAGATATTATGAAGAGTTTGTTTTAAGTATTAATTAAAAGAAAGATTAATTATATGTCTATTAATATTAAAGATTTAATCATGAAAATCGCTAAGGAAAATAAAATTGCTTTAAATATGGATAATTTAAATATCGAATTAAAATCATTAGGAATTGATTCGCTAAGTGCTATGAATTTAATAATGAAAATTGAGGATCAAATTGGCGTTCAATTGCCTGATGAAAAATTATTAAAAATCAAAAATTTACGTGATTTAATTAACGCGTTTGAAGATGTATTAAAATAATTTAAGTATTAATAAAAAATTTAGTATAATATTTAAATGTATGAATTTATTAATACATTTATTAGGGGTATAGTTCAATGGTAGAACTGCGGACTTCAAATCCGCGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCATATAATAACAACAGGACTGACACTTTTTGTATCAGTCCTTTTTTGTTTTTGAAAAAAATTTTATTTATTAATTTGATAAAATTATATTTTCAAACTATCCAATAAATGTTTCTAATACAATCGAACATAAGCAATCAATAAAATTCACATAAGTTGTTTTTGTAAGACGAGTAGTTAAACAATTAATAGAAGTATTAAAGTTCACAAAATAACATAAAAAAAGTATTTTTTACTTGAATATAATCTTAATTTATAGACCATAGCTAATTATTATATTTATAGTTTTAATAGGTGATAGTTTTCTTGATTTAATAGTTGCTAATTGCATAGAAAATTTAATTAACCAACCAACTTTACCATTTAGAGAAAAGTCAGTGACATATAAAATAAGTATCAAATTGAAGTTAGCCTAAAAAATCAATCATTTGGAATTGAAACTGAATTTAATTCAACACCTTCACCAGTTATCTCTTATGGCACAATCATATTATATGCTGTATCGTTATATTTTTACTACATTAAACAACTAAATAAAACAAAACTTCAATTAACATATCAAGATATTTGAACCTCCAGCGAGATTTTTTTTAACTTTTTGTTGATCATATAGGTAATCTTTAAAAATATCTCTTAATTAAGATAAAAATGTTTTTGTCAAAATGTGACCAATAACGATACTAACATCTTTTAATTTAATTGTTTATTGTTTCATATTATCCTCAATATATCTAGAGTAAATATTTTACCTTTATTCTGAATATTAAAAAAGAGTCAAATATTTGTATTTTTATCCAATTAAATCTTTTTTAGCATCTTCTGATCAAACAAAAACTTCTAAACTTTATTATACAAAAAAAACAACATAACCTCTTCTTCTACAAAATTATACGTAGACACTCGTAAATTATGATACAAATAAAAAAGAAGCGAATTAAATTCACTTCTTTTTCTTTATCTAAATTTAAACTAATTAAATTTTTGATTTTGAGCAATACGATAAAATGTTCTTAACATTACACCAGTTCCTTTATGACCAACATCTGCAGTAATAGCAATGTCAAAATGAGCATATGGAACGCCTTCAGTAAATTCTTTTAAGAAACATGCTGCACGTGAAGAACCACCACGAGGATCAGATACTGAGTTAGCAATATCAGCAACATTTGAATTTAACATTTTTAAGAAATCATTGTGGAATGGTAATCTTCAAACTAATTCACCAGCATAATCAGCTGCTACAACTACTTCATTTCAGATGTCGTTATTTGTTGCTCATACACCAGAATATGTATCACCTAAAGCATAAATCATAGCACCTGTTAAAGTTGCAACATCAAATAATTTAGAAGCTTTTAAATCACGAACAGCATATGTTAGTGCATCTGCTAAAATTAAACGTCCTTCTGCATCAGTGTTATCTAATTCAATTGTTTTACCATTATATGATTTCACTATATCATCTGGTTTTTGACCTTCTGGAGCTAATAAATTTAATACTAATGGACATACAGCCACAACATTAACTTTTTCTTTATTTAAAGCTAAAGCTCTCACTGTCATACTTACAATAGCAGAACCAGACATATCAAATTTCATTCAACGCATATGTGATCCCGTTTTTACATTATATCCACCTGAATCAAAACATACACCCTTACCAACAAAAGCATATTTTTCATTTGTTTCTGGATTATTATTATATTCAACAACAACTAAACGAGGCTGTGCTTTATCACTTACTGCAGCTTTACCAACACCAACATGAGCGTTCATTCCTTTAGCTTCTAAATCTTTACGATATAAAACACTTACTTTAACTTCTGGCAAGTCTTTAAATAATTCTTTAATTCGTTCTTCAAAATCACCTGGTGTCATTAAATTAGCTGGCGTATCTTGTAATGTTCTTGTAATTGTTTGTGATTGAGCAATTACATGTAATTTATTAATTAAATCATGATATTTTTGATCAACTACTAAATTATGTATATTTTTTGTTTCAATTTTAGATTTTAGTGAAAATGGCGTTACTTCAACATATTCTAATGCACTAATAACAACATTTAATAAAGATGCATGTCTTGATTCTGGGACAAGTGATAGAAATGAATTTAAATCAACACTAACAGATTTAGGTGATTTTTCTAAAAAACTAATCAATGATCTTGATAGTTCATTAGAATCAAAATCTGGTGTTAAAATTAAGTATTGTTGTTTTTGATATAAACCTCCAAAAACAACATGTTCAGTTGTATTTTCTTCAAAACCTTTTAGGTTGTGATTTTTTTCAACAGCTTTTAATTCATAAACAAATTCTTTTTTTTCATTTAATGTCATAAAATTCCGTTCCTTCTTTAATATTGATAGTTTTAATTTTAATATTCTTATATTATTATAATGCTTTTATCTAAATTATGTGCCTTGTTAGTTTTAATATAAACAAATATAATTATTTATTAAAAAAATTTTGGGTTCTTGATGTTAATCTTGTTTCATGACGATATTCATCAATATCATAATCAGCACTATTGATTTCATTAATAATATTAGCAAACTGTTCATTTGTAATTTTTGCTGTTTGCAATCGTAGTGGCAAATTATCAAAATTGCGAATTAATAACATATCCCCAAAACCAACCAAATTATTACATCAATTACGATCAGTTAATAAAATACTTTCATACGGATTATTTAATTTTAATACAATGGTATAATCAAATACATTTTTAAAAGAAACTGTGCTTTCGTTAGTTATATTTGATGAAATTATTAAAACGATTCCGTGTTTTTTAGCAATATTTGCTATATTGCGAATTAAGGTATCAAAACGTGTATTAAATTGACTACTTACTATCTCAACATATTCAGAAATAATGATTAATTTTTTAGGTAATTTTTGATTTTGATTTTTTAGATTATATTCATCTAAATTATCAACATTATTATCAGCAAATAATGTATTTTGCTTGTTAATATCATTCATAATTTCCCTTAATAAATTAATAACATTTTCAAAATCATCATGATCAACGCTTTTTTTTGTATGCTTTAATTTAGCAAATTCTTTTAATGATTTTGAACCATTATTAATAATAAATAATTCTAATTCATTAGGTGATTTAGTATATAAAGCACTAATAATAATGTTAGAAATTAACATTGATTTTCCAGAAAATTGTTGACTACCATGAATTAAAATACTTCCAGCTTGTGTATCTTCTAATCAAACTACTTTTCTTTCTTTTAATTTTCCAATTCCTAAACATAATTTATCATTGTATTCTAAATTAGTTAGAATATCTTTAATATTAATTTGAGGATTTAAATCTTTGACTTTGGCTTGAATGATTAAAAAGTCATTGTCATTATAAATATTGATTTCATAATCATCTGTATCATTAACTGCTTGTTTCATTAAATTTAAAAAATTTTTTAAAAAATTGTTTAATGATGAACGAGTAAACTGATAAACAATTTCAACATTTGAAAAATAAGGATTAATTGCATTAAAATGATATTCTAATTGGTTATTTTCTAAAAATTTATCGATAATTAATTTTAAATTTTCACCTTTTGATTTAAGTTCATGCAAATAATCTTGACTTTTGCCATCTATAATATCAATTCGTGGATAAAAATTGTACTTGTTATTCTTGATTGTTGATGGATTTTCTCGGAATTGGTTAATATCATTAACAATTGGTTCTAAAATAGTGGTTTTTAGTTTATCATTTTTTGTGATACATGAAGAATTAGTTTGCCGATCATTAAAATTTTTAATTTGTAAATTTTGTTCTTGGATAACTTCTTTTTGCTCCAATTGTTTATCAATATTGTGGTGATCTTCACCAAGTTTTTCTTCGAAAAAGTTTTGTTCACTTAATTGATAATCTTTTTTGTGATAGTTATTGATATTAGTGATTATTTTATTAACTAATCATTTTTTGAATTTTAATCCTAAATGACTTTTCGTGCCAAAAGTGATTAGAATAAAAGAAATAAGAACTAAAACCATAAACATAATTACAATAGTAAAAATTCAAGCATTAGCACGATATAAATCTAAATTAGCATATCCTATAATTCCACCATAAGCAAAAACACTAGTATAATAATAATGTTTTTCATCAATCGCTTTTAAAAATTCTTGCGATCAGCTATAAGCTCACCACTCACATAAATGTAATTTTTTTGAGTGTAAATTTACTAAACTAATATATTCGAAATTATCTTTATGTAAATTAAGTATTTCCTTATTAATCATTTGTTGATTTGTATTAATAATGAATTTATAAGTTAATGAATTTGTGATAATTCCATAACCAACTGCACCAATAATTAAAGCAAATAAAATTAGTGTAATAATATAAAAAACAATCATTCATTTACTAAATAAACGCCTTCTGACTTTTTTAATATAAAAAACAATTAAAAATAATAATAAAATTACATAAACATAGTATTTAACTCATCCAAAAAAAAATTCTAAAACATTGTCTAAAATCGCTCCTGTATATGGGGCTTTTAAAAAAGCAAGTATTAAAATTAAAAAGCTTAATATAAAAAAACAAATTCCAATAAGCTTTTTTTTATTAAAAAAATAATTTTTTTGAACTTTGAATTTTTTTGTCTTTAAATTGCTTTTTTCTTGTTCTTTTCCGTTTGATTCTGTTTTTTGTGCATCTAGTTGATTGTCATAACTATAAAAACTAATTGTTTCCTTATTTAATGTGTTTTTATTGTCACTAACCATACATTACCTCTAAAAATTATTTTGTTTTATAACAGTCAACTACTGTTGGCAAAACTAATGGACGCTTATTAAATTTCTTTTCAAATAATTTAGTTAATAAACGTTTTAAATTTGCTTTGGTATCTTTTAGTATTAAGCGTTTACGTTCATCATAAACTAAACATTCACCCATACGTTTTTTAAATGTTTCTTCAATTTCTTTAATTTGAGCGTCATTATTAACAACTCCACAATAATCAAATTCAACATAATTAAATAGTTTTTGGAATTGTTTATCAAAAATAACAATTAAATTAATAACACCATTCTCGCCCATTTGTGATCGTTCAAATAAAATATTAGCACCAACATCAAGTGCATCAAAAGATGAAATATATTTATCTTCTAACTCAATAATTTGGGGTTTAGGATTTAAAACTTTATCTTCTATAGTGAATAATTCGCCATTAGAAACACTAATAATCTGATCACTTTTAATTCAAGATGTTGTAACTGTAGAACTAAATTTAACCTCAGTTTTATATAATCCATTAATTGGAATTAAAAATTTTGGTTGTAAAAAATTAATTAGATGTTTTAAATCTTCATCAGATTGTGTCATTGGTAAAACTGTTTTAGGTAATGCTTTATAAGGAATGTCTAAACGACCTACTTCATCACTTAGTTCAGCTGCTAACATTTCAAAACCTGGTGTAATAACAACGCCTAATATTAGTTGATCATCAGAAGACAAATTAACATATTTATCTTCGTTATGTAGAATTTTATTTAATTTTGAAAAAAGAGTATCTTGATTTTCAATTACCACAATAATTGCATTTTTTGAATTATTAATTTCAGAAACCGGTAAAGAAATTAAATTTTTACTATTAAATAATTTCAATTTCAATACACCAATAAAAGTGTTAATGAAGTTATTACTATAAACAATAAAGGGGCGATTTTGTTGTTTTGCAATTGTTGCCAAAGTAAAAATACTATATGCATCATTGCTATAACAACCAACAATTAAACGATTTTTAGTATTTTGCAATGCAGCTTCATAAAAAGCCTTATTTTTATGATTGGGAGCAGTAAAATAAGGATTTCCTGCTTGACCCATTCCTACAATCAAAGCTAGAGTATTATTTTTAGTAATGTTATTTAAAAGATTAATTTGTGAATCAAAGGTTTTATTTTTATCATTTGAAATAATAAACTCATCAACATAAACAATTGCTCCATCGCTTGTTTTTAATACAAATCCATATGAATGAGGCATTGAGTTTGAAACTTTAAAAGATGTAACATAAATTGATCCAATTTTCTTATCTGAAATTGGATCTAGCTCAATAATATTAGGTTCAATTTTTTTATTGTTTACTTTTTGTTCAAAAATTTTTTTAATAACAATTGCTCCTATTGGGCTTGTGTAAATTGGGATTTTATAACCTACTTGTCCTAGTAATAATTTAATTCCCATAACATTATTAAATGTTGGTGTACCAATAAAAATTCCCTTAACGCGTTTTGCGTTTTTTTCTAAATAAGAATAATCACAAACAATCATATTTACGCCAAAAACATCACTTGTTGGAATTTTCGCACCAGCATTAAAAATAAAAATATCATCGTTCACTTCAAGAACAAAGCAAGATTTTCCTCTTTCATCTTGTCCTCCTAACGATAAAAAATTAATTTTAGCCATAAAGCTCCTTTATATATAAAAATGTAAAATAATATGTGTTTTAATCCTTAAATTATACTCTATTATATCTTATCGATATAAATATAAATTTTTTAAAAGAACATTATTTTCAACTAATTATTTAAAAGAGGTGATTATAATGAAAATTGTTCAACAAACTTCTGATAATGAATGTGGTGTGTGTGTTATTAACATGTTAGCAAATTATTATCATAATAAAACAATTGATAAAAACATCATTCTTCAAAAAGCAAATTTAACAAAAAATGGTTTGAGTTTGCAAGAACTTGAAAACTTAGCAAATGAATTTAATTTAGATGCACAATCATTTGAATGTAGTTTTGAGGAGTTAATTGATGAAAAAATCAATGACTATTTTATAGCTTTAATTAACAAAAACGGTTTGAATCATTTTGTTATTGTTAAAAATTATAACAAAAATTTTTTTCGTATTTATGATCCAGAAAATAAAATATATGAATTAAATAATGAAGAATTTAAAAAAATATTTTTAAATATAATTGTGCGTGTTTCTAAAAATCATCAAATTTTTGACCTTCCCATATTTAAAGAATCTTATTTAAAAAATATTAAACTCTCATCGTTAATTATCATATTATTTATAGAGTTATTAAATATTCCTTTAAATATTTTTTTAAGTAAAATTGTAAACTTACTAATAGATTTAGTTTTAATTGACACACAAATTAAAAACTTAACATACTTATTTATTGTTTTTAGTTTATTTTATATTAGTAATGCTTTCAAAGATTTACTAATTACTATATACATAAATAAAATTAGTAATAATATTTTTTCTAATATTAATAATAAAATAGTTAATAATTTAGCTAATAAATCAATTATTTTTTTTAATAAAACAAATTTAGGTGAATTAAGCTCGATTCAGATTCATTTGAATAATATAATTCATTTTCTATTAGTTAGTCGTATAAAATTATTAGTTAATACTTTATTTGCAATTTGTATTATTTGTTTATTAGGTTTACAAAATTGATCTTTATTATTAATAAGTTTAGGGTTTAGTTTGTTAAATTTTATAATAGGATTGATTAATTGAAAATATTTTAAAATCCACAGTTTAAAAATAATTAATATTGAAAACTCATTATTTATTAAATATCAAAATTTTATTAAATTAATTAATCTCGAATACAATATCGATAAATATAATAACTATATTAATAGTTATAAAAAGCAATTTGATGAATTAATAATTTTTAAAAATAAAATTACTATTTTTAAATCGAATGTAAATTTTTATTTATCAATTATTAATAATATTGGTTTATTGATTTTATTAACTTATCTAATCATTAATACCAATTATGATTATAAAGTTATTAGCGTAATCACTTATATAGTTTTTTTACATCAAAATCTAAATAATTTGTTTTTTGGTATCTTTAATTTTTTTAATGAATTATTAAATTTTAAAAATGGAAAACAAATTGTTGAAAAAATTTTGTATATTGATAATTATGAAGAACATGATGGTATACAAATTAATAAAATTACATCATGTAAAATTACCGAAAACCAAAAGGAATTTGAATTTAGCAAATCTTGTTTAATCATAGGAAAAAGTGGTGTTGGAAAAACAACATTATTAAAAAATTTTTTAAACTTTAATAATAAAAATTTATTTTTTAATAACTTAAATGTAAATAATATAAATTTAAATCAACTTAAAGAATTAATTATCTATCATCCAACAAATCCTTTAATAAATGATTTTGATATAAATTGGTTTATAGATAAAAATGAAGAAATTATTAATGCTTTAAAAATAGTTATTTTTATAACAAAGATCGATTGATCATTAAATAATAATTTTGAACATAATATTAACTTATCAACTGGTCAACAACAAATCCTTGCTTTTTTAAATCTTTTAAAATACAAAAATAAATTATTACTACTTGATGAACCACTAGCACATGTTGATAATGATAATAAAAAAATAATTTTAAACTCAATATTGCCTATAATTCTAAAAAATAATTTTGTCATTTATGTTTCACATGATCATTATTTAAAAAAATATTTTGATCAAATAATTAATTTAAATGATGAAAAAATGGATAAGTATTAGTCTTTTGGGTTTTTTATTATCTTTATTAATAGCAATTGGTGTAATGTTTGGATTTAAAAATAAAAAATATTTTAAAACGACATTAAACATTCAAACAAAATTATCTATAATCACTAAAAACAATTATGAAAAAATTAAAACCAAAAAACAACTAGAAATTAAAATTAAAAATTTAACTTATTATGTTAAATTTAAATTTTTAAAACAAACTCATGATTATTGCCTAATAAAAATCTTTCTTAGTTTAAAAACAGATCAAAAAATGATTGATATTAGTATATACACTAATACTCAACCATTAATTGAATCTATAATTAATAATTTCAAATTATACAATTAATTGAATAAAAAATTTTTGAATGTTAGGAAATAAATTTTCATCTTGTAAACTGCCTGTAATAAGAAGTGTAAAAATGAACATGATTGATATTACAAATGAATGTGAATCTATACGATCTAATAATCCACCATGGCCAGGAATTAAATTACTATAATCTTTGGTATGATAATTACGTTTAATCGCACTAAATACTAAGTCCCCACTAGTGCTTATTAAACTCAAAAACAAAAAAGCAACTATTGTGCAAATTCATCATAATGCTTGTTTATGCAATGTAAGATTTTTTTCATTTGAACTAGAAAAATCTAAACCAAAAATATTATATAAAACATTTTGTGATTTTAACACTATGCCGTTTTTAGGTTTAATTGGCACATATGACAAACCAAAAATTATTAACATAATAACAATTGTTGCAATTACTAAACCAAAAATTAATCCTTCAACTGTTTTGTTAGGACTTATTTTTGGTGCTAATTTTGTTTTACCAAATAAAACACCACCAACATATGCAAATGTATCACTTAAAAAAACAATCATCATTAGCAATAATGAAGTAATTCAACTTCTAATAAAAGTAAAATAAAGTAATCCTATAAAAAAACCACTTAAAAAACTAAAAATTAAAATTGTGTATAAACAATTTTTGAAATTAATTTTTTTTAGAAATGCTAAAAAGAAAAATAAAATAACTAATGTAACAAAATACGAGCATCCTAAACATGCGCCAAATAGTTTGATGACGTAATGGTAATTTATAGTTAAAAGGTATTTAATATTTAAAAACTCATATTGATATATAAAATAAGAAATCGTTGTAGTATAAATGCAAATATTAAAAACAATTGTTAAGATGATTAATGTTATCTTATTATTATCAAAAATGACTTTATTCATTTCATACGAACCAATACATATTAATGGTAATAATCATATTAATAAAACAATTGCAATCCCTTGTTGAATATATAAATTATTAATTAAAGGTGTTCAATTATAATTACGATCTGCTAATAATGCTAAAACAAAATAAATTAATAAGTATAAAAAAATAAAAATCGATGAATAAAACCGTTTAAAAAAAGTGTTTTTTGTTTTTTCATTGATTTTATTGACACTAATCATTTTTAATTAATTAAACAGTCATTATGTCTTTTTCTTTATGACTTACAACTGTTTCGATTTCCTTATTTAATTCTTTTGTTAAATTATCTAATTCTGTTTGAAAAAACTTTTTATCGTCTTCAATAGTTGTTGAATCTTTTTTGAATAAATCTTGTGCTTCTTGACGGATATGACGTACACGAATTTTAGCTTCTTCGCCAACCTGTTTTGCTTTTTTAACTAAATTTTTTCTTACTTCTTCTGTTGGAGCAGCGAACGTTAAACGAATAATATCAACATCTACTTGAGGATTAACACCTAAATTTGATGCGTTAATTGCAGTAGCAATATCTTTAATACTACTTCTATCATAAGGTTTAATTGCTAAAACACGTGGTTCTGGAACTGAAATATTTGCTAATTGATTAATTGGCATCATTGAACCATAATAATTAACTAAAATACCATCTAAAATAGCAGGCGTAGCTCTTCCTGTTCGCAATTTAATAAATTCATTGTGCATTCATTTTAACACTAATTCAAATTCTTCTCTGATTTTAGTTTCATAAATTTTAAAATTCATAAAATCTCCTTAAAAAATTATTTTGAAACAATTGTGTATTTATTTTTTTTCTCTAAAACATCAACAATTGCATTTGGTTTATCAATATTAAAAACTAATAAATTTATATTATTTTCTTGACACAAAGCTAAAGCTGTTGCATCCATAACTTTTAAATTTTGAGTAAGGGCCATATTAAATGTAATATGTTCATAAAATTGTGCATTAGGATTAATTTTTGGATCACTATCATAGACACCATCAACACCATTTTTGCCCATTAAAATTATACTAGATTCTGTTTCAGCAGCTCTAATTGCTGCACACGAATCAGTTGTAAAATATGGAAATCCTGTTCCAGCCACAAAAATCATTACTTGTTCTTTTTCAATAGCTTTTTTTATATTATTAGCAGATGATTCATGAACTAATTTATCACATTTAATTGCAGATAAAACAATAGTGTTAACATTCAAATGATTTAAAGCATTTTCTAAAGCTAACCCATTTATAATAGTTGCCATCATACCCATATTATCAGCTAAATTACGATCCATATCTAGCTCTTTGGCAATGCTACCTCGTCAAATATTGCCACCACCTAAAACAATTGATACAATATATTTCTTAGAAATTTTTTCAATTTGTTCTGCTAAATCATTAATCTTTATAAAATCAATTATTGAACTATCATTTTGTTTTAAGCAAGCTCCAGAAATTTTAATTACAATTCGTTGTTTTCGCATATGAATTTCCTTTCATATTCCTTATTGACTACACTATAAAAATAGAAAGAAACTTTGTTTCCTTCTATTTTTACATTTAAAAATTATTTTTTCATTTGTGCTGCTACTTCATCAGCAAAACTCATTTGAGGAGCTTTTTCAATTCCTTCGCCTACTTCATATGAGAAATAACCTACTGCAACAGCGTTATTATTCTTTAAATATTTTTCAATTGTTAATTCTGGTTGTTTAAAATATGATTGATCTACTAAACAATTTTCTTTTAAAATTTTATTTAAACGCCCGTCAATAATTTTTGCAGCAAAAGCTGCTGGTTTACCTTCATGTTCTAATTGATTAACAATAATTTCACGTTCTTTCGCTAATCATAACTTATCAACAACTTTTTCATTTAAATAACGTGGACGCATTGCTGCAGCATGCATTGCTACATTTCGAACAACTTCATCTTCAACTTTTCCATCAACTAAAATAATAGCAGCAACTCTGTTGTTATTGTGAGTATAAACACCTAAAGTTTGGTCATCATTAGCTTTTAAAATTTCTCCACGTCTAAAAACGATTTTTTCACCAATATATGCTGTTAAATCTAATCCTGATTCAGCAATTGGTTTTCCATTTATAATTAATTTATCAAAATCTTCTTTATTTTCAACCTTAGCTAATGCTTCTTCTAAAATTTGATTAGCATAAGCTAAAAAATTTTCATTCTTAGCAACAAAGTCAGTTTGACAATTAACCTCAATAAGAATTGCTTTATTATTTATTTTTTTGGCCAATACAACACCATCTGTCGCTGCATTTTTTAGTTTATTAGCAGCCTTAATAGCACCATTTTCTCGTAATCATACTATTGCTTTTTCAATATCATTTTCTGACGCATCTAAAGCTTTAATACATTCACTCATACTAGCTTGTGTTCTTATTCTTAATTCTTTAACTAATTCAGCTTTTGTCATTTATATTCACCTTTATTAAATACATGTTTTTAGCATATCTTATATATTATATATAATTTATTATTGTTTTCATAATATATATTGATTAAATGAATAAAAATACATTAAAAAAAACACTTTCAAAAGTGTTTTTTTTAGATAATTTAATGGTGCGCTTGAAGAGACTTGAACTCTTACACCGCAAAGTACTAGATCCTAAGTCTAGCGCGTCTGCCATTCCGCCACAAGCGCTTAATGGTGCTCTGTGAGGGTTTCGAACCCGCGACCCACTGATTAAGAGTCAGTTGCTCTACCTACTGAGCTAACAGAGCATAAACCCCTACCTACCCAAAGTAGATAGGGGTTTAGCTAAATCCCACTAATAAGCAGGTTGAGTATATAATGGTGCCGACTATAGGAATTGAACCCACAACCTACTGATTACAAGTCAGTTGCTCTACCTATTGAGCTAAGTCGGCATGGTGGAGTGCAAGGGACTCGAACCCCTGACCCTTTGCTTGTAAGGCAAATGCTCTCCCAACTGAGCTAGCACTCCACAAATAATACTTAAATTTTTAAGTATTACTTCGTGTTTTTATAAATGGTGACCTGTACGGGATTCAAACCCGTGAATGCACGCGTGAAAGGCGTGTGTGTTCAGTCACTTCACCAACAGGCCATGGCGGCTACAGTAGGGATCGAACCTACGACCAACCGGTTAACAGCCGGTTGCTCTACCGCTGAGCTATGTAGCCATTTAATAATAATTTTAAAAACACTATAAAATTATAGTTATTTTTTTAAAAAATGTCAATCTTTTTTTAAAATTTTTAATTTTTAATTTTTAGATACATAAAAATCCTTCCTATATATTATATATGTTATAAATTTTATAATCTCAAAGACTATAAAAAATATTTTTAATAAGCGTAAATAGGAGATGAAATTATGAATAAAATAAAAAATGCTAAATCTTCGTTTCTATATTTTAAAAAAATGCATAATTTAAAATCTTTTAACAAAAAAAGAAATAGATTAAAAGGCATACAAATCATATTTATTGTTTTTCTAATAATCTTTTGTTCATTATTTTTCTTAATTGATTTATTTTTTACAGGTAATCATTTGAACGTCATTAAAAGAATGTTTGAAAATAGTTCTCTTTCAAATTCAACTTATATTTTTGTTCCTGTTGCTAATATAATTACAGGATTTAGTTTAGGAATTGGTTCAATTTCTATCCAAATTACTTCTAAAAATATTTTAGCTGGACCGTCAACACTTGGTTTTACACCAATGGCAATATTAGCATCAACCATTTCATTTATTATTACTTCAAGTGGTATTTTTTCAACAATTTTAATTTACTGTTTAGGTTTAATTTTTTCATTTATTATAATAGGTGTTAATTTTATATTAGTGAGAAATAATTTTTTAGAAAATAGTTTTAAACCTATTTTAGTTGCTTTTGGGATTGGAGCATTAGTGACGGGAATTAATATTGTTTTAATTGCAACACATGACAATTTAAAAATTGTGGGATGATGGCGTTTTATTACTATCAACAATACATTAATAAACAATTATCGTATGATTGTTTCAAGTGTTCTAATGATTATTTCTACTATTATTTTATTATTTTTATCTCCTTATTTAAATATTATTAGAAAAGATTATATACTTGCTAAATCATTGGGGATTAAAGTCAATCTAATTTATTGGTTAGTGGCTATTTGTGTGGTAACTATTACTATATCATCATCTATATTATTGGGAATAATTGCTTTATTAGGAGTAATTGTTGGAATTATTATTCAAACAATTTTTAAAAAAACACACGTTTTATTACTAATGATACTAGCAGGACTTTTTGGTAGTGGAATCTTATCATTTTCAAGTTACATTAATGAATATATTCCTAGTGCCCGTGAAATGATTATTTGTGTTTTTGCTATACCAGTATTTGCTTACATTCTAAGTAAGAGAAAAGGATTTATTAAATAAAATGATTAATTTAAACACTTGAAATAAAACTCATTCTTTTAAAAACCCTTTAAAATATCAAAATAAAATCGCATATTTAAAAACAATGATAACAGTAATTATAAGTATTGTTGTATTTATTATTACATTAGCTTGAACTTATGATTTTAATTTTAAACAAGACAATATTGCCTTGGCTTATATAATTATGCAAATTTTTTTATCAGGTTTTATTTTAGGATTTTGTGCATATTTAATTCAAAAATTAACAAAAAACCGCTTCAGTGATACATCAATTATGGGAATTAGTAGTGTTAATATTTTAGGAGTAATTATTATTGCATTTCAGGTTGATTTTAATAATTTTAGTGCTGATAAATTATCAACATTAGAGCATACTGAACCAATAATATTTTTTATAGCACCAATTATTTTATGTATGCTCTATTATTTTACATGCAAAGAACAAAGTAGTTTTAATTATAAAAAAATGCTGATTTCAGGAGTTATTGTTAATTTTTTAAGCGTTGCATTAGGAGCAAGTATTGCTAAAAATTTACCTAAATTAGCTAATGCTTACGTTTCAAGATACACATATGGTAGTATTGAACCACAACAAGAGTCTTTTTTTATCGCTTTAGTTTTAATTATCATAGGGTTATTAATAGTTTTATTTAATTTTAAAAAAATTCAAATAGTTTCATCTAATCAAGACTTAGCTAATCAATTAGGAATCAACGTCAAATTAACTTCTGGACTTCTTTTAGTTGCAATTTGTTTAATGGTAGGAGCATCATATAGTTTGAATGGAAATTTGATATTTATAGGATTAATGGCTGGAAACATGGGTTCTGTTATTAGTAACAACCGATTTAAATCCGCTGCTATTAGTTCAGGATGTTGTGGGTCATTAATATATATGTTGTCTTTTTTATTATTTATTAAAATTTTAAATTTTGATAGTCAATGACTAAATTTAGCCATTCCCTTAATTATTTCTCCTTACTTTATTTATATTATTGTTAAAAAAAATAAATCAGATTTATAGTAAAATATACAAAATATAAAAACAATTAAAGGACGATATATGAAAAAATGAAAACTAGTTCGAACTTCAGGGTTGATTCTTATTACAGGAATTAGTATACCTGTAATAACAACAAGTTGTTCTCTAATTGATTTGTTCAAAAGTAATAATGAGAAACAAAATGAATTTACTCATGGTAAACTAAAATCTAATCTTGATTACAATGTAAGTACTGCTTATTTAAATAAAAGATCTATTGCTTTAAAATTCACAATACCTTTAGATAAAAATCAAGCTATTAATATTTTTGGAACAGGATGAATTTTTGATTTTCAAGAAGATCCATTTACAAAAAACATTAGAACTTATTACTTGGCAACTAACATTCATGTTATTAATTTGATTTCAGCAGCAAAACAAAATGCTTCTTTAACGCTTGGTCAATACGATTTAGAATCCCAATCCCAAGATACAAGAGCTTTTTTTAATGAAAATTTATCATTTAAATCCGCTAAAAAAACTTATAATCCACAATACTATGTAAATTATAATCCAGTAGGCACTTTTTCAATTTTGCCTGTTGGATTAAAAAACCAACTAAAAAATTATGGTATTAAAAACCCAGCCGCTTTAGCTTCTTTAAATAATTTAACAACTGCTGTTGATTTAGCTATTATCAAAATCACACCAGATATCTTACAAAAAAATAATATTGATGCTTTGAGAAATTTTGATAAAGAAGTAGGTATTGGTTTTGGTAATACTATTAGCAATCATCAAAGGTTTTATGCCGCAGGATATCCACATGATGAAAAATATAGTCGTTGAAACCAGTATTCAAACCAACCAGATGAATTATTTAATTCATTTTCAACATCAAATTATCGGAAGGATAATCCAATAAATTTTAGTTATAATGAAGAATATATGACAGTTAGTAACCGTAACACAATAGGTAAAAATAATCCTACTAAATATGAAGCTTATGACTATGCAACAAAAAATTTTGATTTACATTCAGGAGCATCAGGAAGTCCAATAATTGATGACAATAATAACTATGTTGGAATTTATTGAGGTGGATTTAAAATTCAAGAGGGTCTAAAAACAACTTTTTATGGTAATTTTAGCCCTTTATTCGTTCATAAAGATAATGGATTATCTCAAGATTTATTAGCATATTATTTAGCATATACTGCAAATGAAACTACATATTTAGACACTAATTTTAATACTAATTTACGTTATTCAGCATTATCAAAAGAAAATAAAGAAATATATGATAAATTTGTTCAAAGTCTTGAAAAACAATTTCTAAGTGATGATAATCAGTATGGTGATAAAAATATTTTATCACTAAAAACAGTTGATGGATATTCTAAAAATAAACAAGATTTAATTAATACCTTATACAATTTTATTTTTAAGGATATTAATTCACCATCATTTAATGAGAAATATACACATGAAAATTTAAAATTAAAAGATATAATTGATGTAAAAGTTGAATGAAATCGTAACAAAAATATTGATTTAAGAATCACATTTATAACAAAAAGACCGTTAAATTTTAATGTTAATCATATTGTTAATTTCAATACAAGCTTTACTTTTTTTAAAGAAATCTCTAAACCAAATGAAAATAATAATTGAATTGACCAATTATTATAATTTTGTTTATTTCATTGTTTCTATTTTATAAAATATAAATATGAAAATTTAGTTAAAAGGAATAATTATTTATGGCATCTGAAGATAAAGTAGTTTACTATTTATCACCTAATGAAGATAAGGGTTGAAAGATCTTTAAAAAAGGTGGCGAACGTGCAACAAAATTATTTAGCACTAAAAAAGAAGCTTTAGAATATATCAAAACATTAGGACGTAATCAAAATGCTGTTGTTTATATTCAAACAAAAGATGGTAAATTCCAAGACGTTCGAAATTATCGTGATAAATAAACAAGATAAATTAAAGATAAATTAATTGATTAAAAACATTAAGAAGTACACCCTAATAAATGTATTTCTTAATGTTTTTATTTTTCGAATTAAAGGTTAATAATTGCTAAATTCTATTTTCATAAAATATGGCGGATTCATTTAAATGAATATTGATTAGTAATTTGATTATCTTTATAATCGTTTTTATTTAAATACTTGTAGTAGACAATCGTAAACATTAATAAAGAAATAATTAAAATTACAGCACTAGATATTGATAATGGCATTGATCACGCCAATAATCCAGCAAACTTAACACTACTTTGGTAATCAACACCAAAACCAACAACAAAAGCAATTACTAAAACTTGCAATAATAATGTTGGAAAAGGTGATTGTTGCTTTTTAGCAATTAGATCACGATAACGCATTAATGTTAATGACAATGAGTAAAAAATTGCAAATATTGTTAAATGAATATATGTATTATCACCAATTAATAATTTTTTAACTACCTCTGCTGAAATTTGCACAAAAGATGTGGTAATTGGATTATCAATACCATATAAATATTTAAAGTTAGGCCCTGCTGCTCAAATTGGAGTTATTAATTGTTTAATAACAACTTTTCATACCTCAGGATTATGGTTTGTTAAGTGTGCTAAAATCAATCTTGGATCAATATGGGCTTGTGCCCCTAAAAAGTATACTAATCGAGGATTATTTAAAATTCCTTGACGAATTGACATATAAAAATGATCTGGTATTTCTGCATCTAAATTAATATATGCTTCATTTTGTTCTTTAGCATAAACGACTGATAGAGGTTTGATTGAAAAACCAAAAATAATTGCTGCAATCATTAAATAAACAAACAATAAACCAAAAACACAAATAAATAATTGGCGCGAATGGTAACGACGATCACGAATAGTTTGTTTTTGAAAAATATAAAATAGGTAATCAGTAAAACCTAATCCAATCCCTTGAATAAACAACATCGCATGATATCATAAAATTCGACCAGTTTGTAGATTTAAAGGAACAGAATTTGTCATTTTTTGATTAATTAAATGATATAAAGCTAATAGAACAAACATTTTTATAATTGAACTAAAAATCGAAATTAAAGTTTGATTTCAAATTTGTTTAATGCTAATTTTTAATAAATTTAATGATAAACCTAATTTAGATATCCTTCAATCTTTACTAAATTTAATAATACTAATGGCAATTGTTGAAATAATATAACCAATTGACAATCCTAAACCATACCCACTATAAGCTAAAATACTATATTTACCTAATAAAAAACTTGTCACTAAACAAATTGTTCATGTGGCAAAATCTAAAATAATAGTTAATAGAATTTTACGACGATCATTTGTTCAAATAGAAATTAAAAAATAATTATGAACTGGTGCTAAAACGATAATCCCCAAACTTGATCATATGTATTGTTCACCAAATAGTTGAGCAAACAAGGTATTTTCATGAATGTTTGCATAAAGATTATAAGTATAAGATGTCGCCAAATAAACACCCAACATTATTAAACCGAATGAAAATGCATAAAAACTTGCTAAGTTAGTTGTTGAATGTGATGGAACAAACTTGTGTTTACTTCCATCATATAGTTTTTTAGCTCATAAGGCTAAAACTACACCTATATTTCCACCTAATTGTATAAAACTAAATTGGAAAACAACAATATAGCTAACACTTGTCGCAGCAAATGCACCACTAAAATTATGTAACGCTATTATTATTGTAATTAATAATAAAGCTAACGATGAGAACAAACTTGAAGCTATAAACGATGAGCTATCTTTTATGACTTCTCATAATGTTGTAAAAATGCCATAATGTTTTTTAAAAACATGACGTTTAATTACAAGGGGCGTTTTTTCAATCGATGATGTCATAATTTTCTCCTTTTCAAAATAAGTAATACATTTAAAAATTATACCATTAATATAAAAATATTATTTAATATGTGGATATTAAATAATATTAATTTTGACGAACCTTAATTTGTTGTTCTAATTGTTTTTTAGGCATAATTGATAATTTTTCATCATTGTACTTATGATTCTTTAAATATTTACGATAAATACGTAAAATTTTATAAGTGCTATATCCTAAAATAAAAATACTTGATATAAATAAAGGAAATGATCAAGCCATTAAACCAATGAAATGTGGATCTGATTGGTGTGTTAACCCAAATCCAGAAGTAAACGCAACCATAACTAATTGAATAACAATCATTACATATGGAGGACGTTGTCGTCGGGCAATTAATCCTACATAACGACTTAAAATAGAAGAAAAAGAAAAGAATGTACAAAAGAATGATAATAAAATATAAGCATTATTTTTAATTAAATAACTATAAATATTTGAAGCTGAAATATCAACAAATCGATGTGTAAAAGGATTTTTCATTTGATATATCGTTTCAAATTTATCACCTAGTTTTCATATTGGATCAATCACTTTTTGCGCCATTGGTAATCAAATATTTTTATCATTTGATGATAAAGCATTAACAACTTCTTGGCGACCAATATTCACATGACTAGTAATAATATCTAATAATTTTTCATCATTAATTAAAATTTTTCTTAAAGATTGATAGAAATGTTCGGGAACTTGCTTTTCTAAAAATATATAAGATGGACTTTGTTCACTTGTATACAATTGGACTAATTGTTTTACAGAAAAACCAAAAATAACTGAAGTAATTAAAGCAAATAAAATACCTAATATAAAAACTCAAAAAAATAATTGTCTTGAATGATAACGACAGTCTCTGACTTCTTGTTTTTGATATAAATAAAATAAAAAATCTGACAATCCTAAATTTAACATTTGTATGAAAATCATTGATTGGTACATTAGAATTCTCGTTGATTGAAAATCTAATAATGATGAACCAACTACACGTGAGTTAATTACCACAAATGTAATTAACAAAATAAACATTTTACCAACTGATTTTAATGATGATAAAATCGTTTGAATTCATAATAATTTAATACTTAAAACAAATAATCTTTTTGAAATATATAATGGACGTAAAACAATTTGGGCTTTTTCAATTGTAGTCATACCAATAATAATAGTTCAAAAAACAAAACCAATTGACATACCAAGGCCATAGCCCCAATAATTTAAAACACTATAACTACCTAATAAAAAAGCTGCAATTAATGCGGTTGTTCATGTACAAAAATCTAAAACAATTGCTAATAAAACATTAATGGACTTGGTTGATCTTATTAACATCAATAAATAGTTTCGCAAAGGTGCTAAACCAACAACTGCTAATCCTGAAACAATATATGCACTACCTACACTTTGATTAATCACTGTATTTTGGTGATCATTTGCAAAATGATTATAAATGTAAGAGGTTGTAAAATAAATTAATAAAACTACTAAACCAAAGATAAATGAATAAAAACTAGCAGCATTTACTGTATCATTTGTAGGTTCAAACTTATACTTACCATCTTTAAATGATCTTTTTGACCAAATCGCAAAAACAATGCCAAAGGTTGTTCCTAATTGTAAAAAACTTAACTGAAAGATAGTTAAATAACTAATACTAGTTGCTGCATAAGCACCAATATCTTTATGATGAGCAACAATTACAACAATTAAAATTAATGATAGTCCTGTAAAAACACTTGCAGCGATAAATGGCGAGCTTTCTTTTAAAAGTTCTAAAAGTGTTCTAAAAACGGTAAGATTTTTTTTAAAATAAGAATCGGATTTTTTAATAACTTTCATTTGTTTCTGTTCTTCCAAATTAAATCTCCCCCTTTTATATAAAAACACCATATAGATTATATCATAAAATATACTAAAAATTATATTTTTTTATTTATTAAAAATTTTTTAATTATTTTTATATAATCATCGATTCAACTATTAAAAATTATATTAACTTCTTCTCAAACATCGTTCTCATTAATTTTTATATCTAAAATATTAATTGTTTCAAGAGGAGACAAACTCCCTCCAGATTTAAAAAAACAGAAATATTTTTCTTTAGCGTTTGCTTTATTATTGAAAATTCTTATCGCATTAATTAATCCACAAATTTGTCCAATAACATATTTATATACATAAAAATTACCAGTATAAAAATGTGGAATGTTAATTAAAATTAAAGCGTTTGCTTCATCATATTTACTGATTTTGTTTTTATTATATTTATATCCTGTATAATCATGATTTATTTCTAGATAAGCTAAAACTATTTTATTTCAGCTAAATTCTTCACCTTGATTAATTCATTCATTGGCAACTCACTCAAAATTTGAAAAAATCGCTTGGCGTGTAGTTGTAGCAATAAAACCACTAATCATTTCATCTAAAATATAAAGACGCATTAAATCATCATTTTCGTATTTCTTTAATAAATGGTAATTCATTAAGATCTCATTAGTAATGGAAGCAATTTCTGCATAAAAGGTTTCATATTCATTATATACTTCTTGTGATTGATTAGCAAAATAAGTGTGTACAGAATGCCCTAATTCATGAACTAATGTTAAAATAGAATTATAAGTTTCATCATAATTCATTAAAATAAAAATTTTATCTAAGCCTTTAGTGTTACTTATAGAATATGCTCCAGAAATTTTATTATTATTTGGCATTCATGATATTCATTGCTCATTGAACGCTCTTTGTACAACATTTATATATTCACTACCTAATAATGCCAAAGCTTCTAAAGTTAAGTTTTTAGCAGATTCAATACTAAACATGTTTTTTTTATCAATTATATCTAAATTTTTATCTCATGGTTCAACTTTAGTTAATTGATATTGTTGTTTTAGAAATAATGTTCGATATTTTGTATATCTATTAATTCCTTTAGCAAATTTTTTTGTTTGTGTATAAATATGATTTATAAAATTTTTATCAACCTTATCACTAAAAGCATCCGCAGAAATATAATCCTTAAAATTATGTAATTTTGCTAACTCGTTTTGTTTAACATATTCATAATATAATAATTTACTAAAAGTATTACGTAAATCATATATCGCTTTAAAATGAGATTCATAAGCTGATTTTCGTAAAGCACGATCATTTGATTTAGATGCAACATAAAGATCAGTTTGATTTTTAAAACAAACTTTTTGTTTTTTATAGTTTATACCATCCTGATACTGCATATCTGAATCTAATAAAACATCAAAAATATCACCAAAACTTGAACTAAAACGGCTAATAGCTGTAACAACTTTTTGTTGTTGTTCATTTAAAATATGTTTTTCGTAACGTCATAATTCTTCATATTCTAATTGGTATTTAATTAATGAAGAATTTTTTAAATATGAATTAATTAAATCTTTATTTTTTATTGCTAAATTTTCAAAATTAATAAAAATTTTAGCTACACGATGTTGTTCATGTTCAATTTTTTGAGACCAAGCAAGCATTTCATTATCGATTAAATTTGTTTGCAATTTATTTGAAACATAATTGTTAATTCGATTACTAATTAATAAATTTTTTTCTGAAACTTCGTGAAAATTAATAAAATCATTTAAACTATTAATCCACTTTTCAAAAGCTTTTATTAATTCATTATTTGATTCAACAAATTCATTGAACAAAATATCTAGTTTCTTGCCTTTTAATAATGAATCTATATCTCAATCATATTTTTTATCATTAATATCATTCATTTAAACTAAATTTCCTTTTATAGAGTTATAGAAAAAAATGACCAAGTTTACTAAAAGACTTAGTCATTTTTTGTTTTTTGTTTTTTTAAAATTATTAATTACTTGTTTTAATTACGCTACCATGACCTACAGTTTTACCACCTTCACGGATTGAGAATTTAGATCCATCTTCAATCGCAACTGGAGCAATTAATTCTACAGTCATTTCAACGTCATCACCTGGCATAACCAAATCAACACCAGCAGGTAATGAAATAGCACCTGTTACATCTGTTGTTCTAAAATAGAATTGTGGACGGTATCCTGAAACAATAGGTGTATGACGTCCACCTTCTTCTTTTTTAAGAATATAAACTTTAGCAGTAAAAGTACGGTGAGGTTTAATTGATCCTGGTTTTACAAGTACTTGACCACGTTCAACATCTTCTTTTTTAATACCACGTAATAAAATACCAGCATTATCACCAGCTTCAGCTTGATCTAATGATTTTCTAAACATTTCAATTCCTGTAACAACAGTTTTTTGAGTGTCTTTTAGACCAACAATTTCAACCTCATCATTAACTTTTAATACACCACGTTCAACACGTCCAGTTACTACTGTACCACGTCCTGAAATTGTGAATACATCTTCAATTGCTAATAAGAATGGTTTGTCAGTACTACGTTCTGGTAATGGAATTCATGAATCAACTGCGTCCATTAATTCATCAATTTTTGCTTCTCAAACTGGATCTCCTTCTAAAGCCTTAAGACCTGAACCACGAATAACTGGTGTGTTATCGCCATCAAATCCATATTTAGATAATAATTCACGAACTTCCATTTCAACAAGATCTTGCATATCTGGATCTGTCATGAAATCACATTTGTTTAAGAAAACAACGATTTTTGGAACACCAACTTGACGTGCTAATAAAATATGTTCTTTAGTTTGAGCCATAACCCCATCAGATGCAGCAATAACTAAAATTGCTCCATCCATTTGTGCTGCCCCTGTAATCATATTTTTTACATAATCGGCATGTCCTGGACAGTCAACGTGTGCATAGTGACGTGTTTTTGTTTCATATTCAACGTGAGAAGCGTTAATTGTAATACCACGTTCTCTTTCTTCTGGTGTTTTATCAACATCAGCATATGATTGTGCAATAGCTTGTCCTTTTTTGGCAAGTACAGTACTAATTGCAGCTGTTAAAGTAGTTTTACCATGGTCAACGTGGCCTATAGTACCAATGTTAACGTGAGGTTTTGTTCTTTCAAATTTAGCTTTAGCCATTTTAAATCTCCTTAAAAATTAATTTATTTTCTAAATAATTTTTGAATACATAATAAAAGGTATTATACCTTATAATTATAACATTTTAACTTTTTAGATTATTAATTAAATACTTTATTGTTAATTGTTAAAATAATTATTTTTTTGCACGTGCTACCATTACTTCTTCAGTAACAGACTTAGGTGCTTGTGCATAGTGACTGAATTGCATAATATATTGACCACGTCCTTGTGTATTTGAACGTAAATCCGTTGCATATCCAAACATTTCTGATAGCGGAACTTTAGCTTTAATAGCTTGTGCATTACCACGTTGTTCTTGTCCTTCAATTACACCACGACGTTTTGAAATATCGCCCATTACCGTCCCAAAATAATCATCAGGTGTAGTTACATCAACTGACATAATAGGTTCTAATAAAACTGGTTTGCATACTTTTGCTGCTTCTTTAAAAGCAAGAGATGCAGCAATTTTATAAGCCATACCAGATGAATCAACATCATGATATGAACCATCAAAAATAGTTGCTTTAACGTCAATTGTTTGATAACCAGCAATTGGCCCTGATGTTAATGCCTCAATCAAGCCGTTTTCAACTTCTTTAATATATTCTTTTGGTACTTTACCACCTACAATGTTGTCAACAAATTCAAATCCTTTATCATGATTAGGTTCAAATTTAATTCAGACATGCCCATATTGACCACGTCCACCAGATTGCTTAATATACTTACCTTCAGCATCAGCAACTTCAGTAAATGTCTCACGATAACTAACTTGAGGAGCACCTACATTAACTTGAACATTAAATTCACGACGCATACGATCAACCAAAACATCTAAATGTAATTCACCCATTCCAGCAATAATAGTTTGACCTGTTTCTTCATTTGTGTATGTTCTAAAAGTTGGATCTTCTTCAGCTAATTTAGATAAAGCTAAACTCATCTTTTCTTGATCTGCTTTAGTTTTTGGTTCAACAGATAAAGAAATAACAGGTTCTGCAAATACCATTTGTTCTAAAATAACTGGTTTTTTTTCATCACAAATTGTATCACCAGTAGTAGTGTCTTTTAATCCAATTACAGCACAAATATCACCAGCACTAATTGAATCAATCTCATCACGGTTGTTTGAATGCATTTTTACAAGACGAGAAATTCTTTCTTTTTTATCTTTTGTTGCATTGTAAATATAAGTACCTTTGTCAAGTTTACCACTATATACACGAATATATGTTAAACGTCCAACAAAAGGATCAGTTGCGACTTTGAAGGCAAGGGCAGCAAATGGTTCATTTGGATCATTATGATATTCAATTGGATTACCATGATCATCAACACCTTTAATTGGTGGAACATCAATTGGTGAAGGTAAGAAATCAACAACTGCGTCTAATAATTTTTTAACACCTTTGTTTTTAAAAGCTGTACCACATAATACTGGGTATAATTCAGTTGAAATTACTCCTTTACGAATACATTTTTTAATATCTTCATGACTTAGTTCTTCACCAGATAAGTATTTTTCCATCACTTCATCATCAAAAACAGCTACATCATCGATCATTTGTGAACGCATTACTTGTGCTTTTGTTAAGTAATCAGCTGGAATATCTTCAATTTTATATTCTTCATCAGCTTTTCCATCATAAATATAAGCTTTCATTTCAATTAAATCAATACTTCCAACAAAATCGGCTTCAGCACCAATTGGAATTTGAATAGCTGTGGCTTTAACACCTAAACGATTCTTCATTGTCTCAATTGAGTAATAAAAATCAGCACCTGTTTTATCCATCTTATTAACAAAAACGATTCTTGGAACATTATATTTAGTAGCTTGTCTTCAGACTGTTTCAGTTTGTGGTTCAACTCCATTTTGACCATCTAGAACAGCGACAGCTCCATCTAAAACACGCAAAGAACGTTCTACTTCAACAGTAAAATCAACGTGTCCCGGTGTATCAATTAAATTTAAACTATGACCTTTTCATTTTGCATAAGTTGCTGCTGATGTAATGGTAATTCCACGTTCTTTTTCTTGTGCCATTCAATCCATTGTTGCAGCACCATCGTGAGTCTCTCCGATTTTATGATTCTTTCCTGTATGATATAAAATCCGTTCAGAGGTTGTTGTTTTACCAGCATCAATGTGTGCCATGATTCCAAAGTTACGAAATAATTTTAATTCTTTAGACATATATTAACAATTCCTTAAAACAAATTTTATCAACGTAGGTGTGCAAAAGCTTTATTTGCTTCTGCCATTTTATGAGTATCTTCTTTTTTCTTAATTGCTGCTCCTACATTATTTGAAGCATCAATAATTTCATGTGCTAATTTCTCAATCATTGTATGTTCGTGTCTTAAACGTGCATATAATGTAATTCATCTTAAACCTAAAGTCTGACGACGTTCTGGCGTTACTTCAGTTGGTACTTGAAAGTTTGAACCGCCAACACGTCTTACTTTAAGTTCAATAATTGGCATTACATTATCAATCGCCTTATTAAAAACTTCTAAAGCTGGTTTGCCAGTCTTTTGTTCAACAATCTCAAAGGCTGAATAAATAATAGATTGTGCTAATCCTTTTTTACCATCATACATAATAGCATTGATTAGTTTAGTTACAAGACGAGAGTTATATACTGGATCAGCTAAAACTTGTCTTTTTTGTGGTTTTAATTTTCTCATTTTTTTCGATCTCCTAACTAAATTTAATTATTTAGATGCTTCTTTTTTAGGACGTTTAGCACCGTATAGTGAACGTTGTTGACGACGTTTTTCAACACCTGATGCGTCGCCAGCACCACGAACAATATGATAACGAACCCCTGGTAAGTCTTTAACTCTTCCCCCACGAATAACTACGACACTGTGTTCTTGTAAGTTATGTCCTTCACCTGGAATGTAAGCTAAAACTTCAAAACCATTACTTAAACGAACTTTAGCATATTTACGTAAAGCTGAGTTAGGTTTTTTAGGTGTCATTGTACCAACTCTAGTACAAACACCAGATTTTAGAGGTGATGGGTTTTTTGTTGTTTTTTTATGTAATGAGTTGTATGTAAATAAAAGAGCAGGTGATTTAGTTTTTTTTACTTTAGGTGCTCGTTTATTACGAATCAATTGCGCAATTGTAGGCATGTAAATTTCCTTTCATTTTTTATGTTAATTATAGACAACGACTCCGTGCGTATCAAAAAAGTAAAATTACGATTGATAAAACACGTTACAAATTATAAACGAAAAAATTATAAAAGTAAATAAAAAAGAGCAAAAACAAATTTTTTACTCTTTTTTTAGGATTTAAATATGAAATTAAAAATTATTTATTTGCTTTTTGGTTCTCAATATAGTTGTTTTATGTAATCTTTTAGTGCTTTAGCTTGACCTTCTTTGCCTAATCCATAATAGCCTTTAGCGATTCCTGCTTCAAAACCACCATAAGATGAATTACCACCTATTAATACATAAGATTCTGATCAAGTTTCATCAGATTTCACCATTTGACCGTTTTGATTTTTTTCGTATTTAACACCTTTATTATTTATATCAAAATTACCAAATAATTTACCATATTCTTTTAATAATGCTACACGTTCACTATTAAGTTTACCTTTTGAAGCATTATCATTAAAGTCATCAAAATTATCACCAATACGCATTACAGTTTTTAAAACTACAGCATTACCACTACCGAATTCACTTAAATCATAACCTTGTGTACGCTTATTCATAGCATTCATACGGTCTTCTTTAGTAGATTTTACACGTTTTCCTTGTGCATCTTTTTTAATATTTGCTCATGGTTTATCGCTACTAAAATCTACTCCTTGCATTCAAAAAATTCATTTAGGTAATAAACTACGTTCTAAGCCTTCACTAACCAAGTTTTCAATAGTTGGATCGACTTGGTTTTCTTGTTCACGGTTTGAGTTGAACATTACGACTCCACCATGTTCTCAAACATATTTAATGAATTCAAAAGCTCCAGCTAATCGTTTTGAAACCTTGTCATTCACAAATGCATCTCAATCCTTTGGATTATAAGCTTTATTATTTAATAATTGATAGTTTTGATTAGCGTAATTATTTAAAATTGTTTCATCAATATCCATAAATACTACAGGAATTGCCTTATTACTTTCAGGATTAGAAACTGTTACCTTTGTAATATTACCATTTGAATCTTTTTCTACTTTTACTTTACTTGTATCAAAATCACTTTGTTTAATCATTGCATCAAATTGGTGTTTTGCACTATTATACTCTGTTAATAGCATCCCATCTTTTTCAGCACTTAATGTGTTTCAAATATTAGCTAATCAACGTTGGTTGTAAATAATTGATTCTGCACTATTATATGATTTTATTTCATTTTTTTCATTACTATGACTTATTTGAACCTTTGGTCCATCATTTAATTCAATATTGCTTATATTTAGTGTTGTTACATAAACAGCACCTTTTTTTAAATTAGCATCATCTGGTTTAATAATTACACGATCATCTTTTTTTGGTTCACGTGCTAATTTTACATAAACTCTACCATCCCTTAAAATCCCTGTAGATGATGTAAATTCAGCATTATTATCTTTATCAATAACACTAGTTTTAAAATTAAGATTTTGTAATGATTTTTTTTGGTTATCATCTAATTTAGTTCAATTTGTAATCTCATAAATTGCATAAACACCATTTTGAGTTCTAACAAAAGATGAACTTACAGTTGATTCGATATTAGAGTTAGTACAAGCAGTAGCTGTAGCAATAATACTTGTACTAACTATTATTGAACCTAATGATATAGCTCAAAATTTCTTATTTTTCAATAATTTCATAAAAACTCCTTAAACAAAAAACATCTTTTTTAAATTTTTAATACCAAAATTATAGTATGAAAAACCAATTATAAAGTAATGAAAAATTAAAAATATTAATAATAAAAATTTATTTATTTTTTTAAAAAATTAATTAAATTAAATCATGAATCTTATTGTATAATTATGAATTGTATATACAAGTAAACTACAATTTTTACTTTATAATTTCTATTCTACATCTCAATCTTCATTAAATAAAGCAATTGATAATACCAACGCAATAGCTTGAGTTCGTTTTTTAATTTGTTCTGGTTGAAGTTCTTCTTTATCACTTAATTTTAAACTCATAAGTGCTTCTTTAAATACATCTTCATTAACATCAATTTTATTAGATTTTAACTTTTCATTTATTTTTGTGAATTCATTGTAAAGTTTTGTTATTTGTTCCTCATTTAATCCTTTGTATAATCAAAGATTTTGAATGATAGGTGCATTTTTATAACGTTGTTTTTTATCTTTAAATTCTTTATTATTAAGAGCTGAGTTGTATTCTTTAGTAAGTGGTATTAAATTACCAATATTATTTTTAACAATATTAAATCTTTCATCTTTTAATGATTGTGAATACATGTGTTCAATTGTTAAATCTGCTTTTTCATTATCAGCATTACTTGGTGGATAAAAAAATACTTTTGTATCCTTATTTTTATTTATTTGCGTATCATCAAGAAAAAAAGTTAAATAAGTTTCAAATCTAATTAAATAAGCCAAATGAATATCACTAACCTTTTCATCAAGCATTTTGTTATAAAAATTTTTAATATTTTTTATTTCCATTTGATTATTGCTATTAAAAACATCTAAAAATTTTTTTGATAAATTAATATTTTTAATGGAATTTATATTGTTTTTGTTAATATCATTATCAATTAACTTAATTAATTTAATAACACTATTTAAAATACTTTGACCACGAGTATTTAATAAAACATTTCTCAATGAAAAATTAGCAATCACGCTAACATATTCTTCAATATTAATTCTTAATTTGCTTTTATCAAAATTACCATTGTTATCAAAATCATACAATACATCCTCAGTAAATATTTTCTCTAATAAATAAACTAATAAAGGAATATAATTCTTTTTCTTTTTGTTCGTTAAGATTAGTAAATAATCACTAATTAAATAAAAATTACAATTATTGTTTGTGAATTTATCTTTGATTGTTAAGTCATTGTAAATATCTATATATTTACTAAAATATTTCAAACACGCTTTTCAATTATTTTTTAAAGTAAATTTTTTATTAATAATTTCAATAATAAAAAATCAACTTTTATTATAATATTCATCATCTTTATCGTTTTTTTCAAATCTTTTTAAATCCTCATCTTTACAAAGATTTAAAGCACTTAATGAATCAACAAATTCGTTAAAACGTTTTACTTCAATAATATTTTCATCTTTTTTTAATAAAAATTTTTTCGTTATTAAATCATTATATTTCTGTTGGAATTCTTCTTTATTTTTAAAAATTGATTCTTTAAAATTTTCATATCAACAAAACTTATTTTTTAACAATTCAATAGCGTTAAGTTTTTTAGCATTAGTATTTAATGATTCAAAAGTTTTAAATTCATCAACATTTTTTAAAATAACTAAATTGAGGATTAAATTATTAAATAAGGCGTTAGCAAAATTCGCTCATTCTTTAAATTCTTTTGGTTTTTCTTCAAATCATTCAACAATATCTTTTAGATTCATAAAAATATGATTGTTTTTATTATCTAATTCATCATTAGATAATATTTGTTTTAAAGCTTCATAGTCAGGATTGCCACCAATACGACTAAAGCGATCAATTATAAACTCTCTGTTAAAAAAGATATCTTTTAAATGACTTAGTTGCTTTATTTGTTCGTTTGTAAATTTTGTTATTTCTTCTCTGTTGTTATACAAGAAAATAATAAATGCTTTTGTAATTAAAAATAAGGTTGTTAATCGTTGTTGACCATCAATAATTTGAATATTATCAATAGATTCTTCTTTAACTAAGACACTAATGATACTACCTAAAAAATGGTTTTCATTATTTTGTTTGTTACTAATATCATTAATATCTTCTAGCAATTTTTCAATATTATCAATTGTTCAAACATAATTACGTTGAAAAACTGGAATACTCATTTTAATTGTTTCATTCTTGTTTAAAAATTTAATCAATCATACTAAATTAAAAATATAAGCATCACCATTATCTGAAGCCAACTTCTTTGTACCATCTAATTTAGTCACTTCAATAGAATTGTCATTTGAAATTAATGGTAATTTTCAATTAGTAACTAATTCACACGAATCACTATATTCTTCAAAATTAAATTTTTTATTATCTTTTTCTTGTTGAGTTTTCGCTGTTCTTAAAAAATCTGGTTTATTTTTAGTTTTATTTATATTAGTTTGATTGTTTGGATTTGCTTTATTTTCATCATTAGTTTTTGCTCAATAGTTTTCAAATTTGGTAATTAATTCATTAGAAATTATTTGATCTTTAAAATAATTTTCAACAAATTCTTTATATTGTTTAAAATGCTTAAAATTGCTATCATTTTGCTTAGGTTCATAGTAGTATTCAAATAATTCTTTTTTAGTTAAATAATCATTGTGTTTTTTATAATGTAGTAATGATAAGAAAATAGTTAAAACAAAATCTTTATTTTTTTGATCTATTGTTTCATTTTGAAAAAAATCATTTAAATCTAAATTTCTTAAAAATTTATTAACAAAAACATTTGTGATGTGTTTATTGATGCTTTTTTCGGTGTTATCTGATACATTAAAAGTAAATATATAATCTTTAAGAGTGTCATTTTTGTATGTATTAATTGTTTTATTTTTATCACTATCTACAATAATTTGTAAATAATAAAGAACACGTATTAATTCATAAGATGAACGTTTAAAACTAAATTCAACTTTTTTTTGATTTTTATCATTAATTAAAATTTTTCATTCTTGATCATTAGTATCAAGGTGTAATTTCTCATTCGAAAAAATCTTTTTAACATTATTTTCATTGCAGATCAAATATTTAATAAATGTATTAATACTTGTACTTATTTTTCAAAACTTTAATTTAGCCATAAATTATTTATTAAATCTTTCTTGAATTAATTTCATTAAATAAACCATTACATCAACAACAATTGAATTTCCAGCTTGTTGATACATTTTAGCTTTTGATATTCCCTTAGCTTGAATCGCTTCAAAATCTTTTTCATTAAAGCCCATTAATAGATAGGCTTCACGATTTGTTAATAAACGAAAGTTTGCTTTTTTAGGTAAATTATTGTGATCTTTTTCACGATAATCATCACCTAAAATCGTATTTTTTAAATTGATATAACCACCATTAGGAAAACGATCTTGACGTGTTGTAATTGTTGAAGTACAATAACAATTAGGATTTAATAAATCATATTTTGGAATGACATATTTTATTCTTGAAAATGTGCGATTTGGTTGAGCTAAATATGCTTCTTGATAATATTTTTCAATTTTATAATTAGTTTTTAAAACATCCTTTAATTGTTTTTGTTTTCCATCAAAAATAGGTGCTTCTAAATCTAAAATATTTCCATTACTATCAATTAATCCATCATAGTTTAGAATCGAAATTGCATATACTCGTTTACGACGTTGTGCTGACCCATAATCAAGAGCGTTTAATTGAAAAGTACATGTACTATAACCTAATTGTTTTAAAGATTTGGTTCACATATCATAATCTAGCTTATGTTGTTTAGATAACATGTTATTAACGTTTTCTAATAAAAGAAATTGTGGTAATTGATTTAATTTTTGCAAATCGAATAAAATGCGTTCAATTTCTCATAGTAATCCAGAACGTGTACCGGATCCTTTTTTCATTCCAAAAGCTTTTTTACTAAAGCTTCCAGCTGTTGATAAGTCTTGACAAGGGAATGAATAAGTAAGTAAATCTATCCCTTTGTTCCCAACACTTTTTATTAACTGCTCACCAGTCACACTTAAAATACTTCCTAAATTATTAGAATTAAGATATGCATCATATAATTCACGTTTAATATTTTCGCTAAAAGAACTAATTTTTTTACGAATTGGGTCTTTACCATTTGATGAGTGATTAAATTTTTCTAAATAATTATCAATTCTATTAATATCAATATTTAAATTAATTTTTTTATGTGGATAATGAATTGTGTTATAAGAAATATTTGCATAAATATCTCATTCGCTTGTAGCAACAATTTTGTAGTCAAAGCCAAATTGTTTTTTTACAATTTCTAAAGCACGATGTTGTGCTCCAATTCCAGAAAAAGTTTCAAATACTCTTAAAACCTTAGTTTTATCTATGTTTTAACATTGAAATTATTTAAAAATGCTAAAATTAAATAATAATTGATGTTTTTGTATTAATTTCGTACGAATAAATTATTTAGAACAAAATGTGGACATTTATGAGCAAAAATAAAGCAGATAAAACTAAGGTTTTCTCTATTTTTTTGTAATTTAAATTAATATAATTTTAAAAATCCTATTGTTTTTTATATATTTATAATTTTTACATATGAAAAAATTTATAAATTATTTAAAAAATGTAAAAGGATATAGTGATGGTACAATTGCTATTTATCAAAAATATGTTAAAGTACTAATTGATAATAATTTAGATTATTTAAAAGCTATTAAAAAATATGAACATAACGCTAATGTTACTAAGCGCGTTGTTTTAAGTGCTTTTAAAGCATATTTAAAATTTATTAATCATAAAGATGCTATATTGATTGTTATGCCTAAAAAAGAAATTAAAGTAAAAGAATTTTTGACTTGAGATAATTTTTTAAAAATTATTAATAATATTAATCAAGCTACCTTTTTAGGTTTTCAGAAATATTTAATTTTAAGAATTTTATTTGAAACAGGCATTCGATCTTGTGAATTATTAAAACTAGAAATTAAAGATATTTATGAAAATAAGTTAAAAATTCATGGCAAAGGCAAAAAAGAACGCTTTGTTTATTTATCAAACGATTTACAAAATAATATTAAAAAATATATTGGTCAAATTAAAACAAAATATTTATTTAATTTTAGTTATAAAAATTTGTATCAAAAAGTTACTAGAATTAAAACTAATATTAGGCTAACGCCTCATATTTTACGTCGTAGTTTTGCTAACCATTGCATTAATAATGGTATTAATATTTATGATTTATCATTAGTTATGGGTCATAACAATATTAATTCAACAAGTTTATATTTAAATAAGGAAAGTAAATTAGTAGAATTAGATCATCTTTTTGATAATAAAAAAGTTAATTAATTTTATAAATATATAAAATAAAAAATCTACTTCAGTTTTGCACTAAAGTAGATAATATAATTCAATTCTAAATACCAACAAAAAACTAAATTAAAAATAAACTTTTTTTAGTTTTTGTTAATTAATGTCTTAAAGACCTAAATAATTTTTTAAAAAATCTAAAATATCTTCAGCTTTAATTTTTAGTTCTTGATAAAAATCTCGTTTTTGATCTTTGCTAAAATCATATAAATCAATATATACTTTAAATTTAGGTTCTGTTCCAGATAATCTAAATTTGATTGTTGAGTATTCGTCTAATTTTCATTCAAGATAAGATCCTTGTTCATGAAAAATAATGTTATGAATGGTGTATTGGGTTACTGTTTTAAATTCAAAATTTCTTAAGTTATTTAAAATAATAAGTGCTTTTTGTTTTCAGTTATCACCATCAATAACAAACTGAGTAGTTTTACTATATCAATTCCCATACTTAGCAAAAATCTCTTTTTTTAAAACGGTAAATAAATCAAGTTTTTGTTCATAATAATAATTAATCATTTCTAACAAAATAGACGCAGTTTGATATGAATCTTTATCATTATTAATATCAGATGGTAATGAACCAATTGCTTCTTCAAAACCAACAACCAAATTATGTGTTTTTTTACGATTGGCAATTAAATTACCATGATTTTTAAAACCTACATCTACATATAAAACATCACAATCAAAATTTTTAGCAAATAATTCTGGATAGCGTGTTGAAATAAATGTTGAAATAATATATGTAGGTTTAGTAAATTTTTTATGTTCTAATAAATAATGCGCAATTAAAACACCTGTTTGATTACCAGTCAAAAATTTTCAATAATTTTCATGACGTACAGCAATGGCCATTCGATCTCCATCAGGATCAGATGCAATCATTATTTCTGCGTTTGTATTGTCTGCATATTCAACACTTAAATCAAATGAACATTGTTCTTCTGGATTAGAACTTGGGTCATCATTAAAATTAGGATCTTCAAAATTTTGTTCGCTAACGCTAACCATATCATAACCAAGTGATTCTAAAAAAGGAATCATGTCCTTTGTTGTTGTTCCATGATGACCACTAAAAACAATTTTGAAGGGTTTTTTAATATTAGGATCAGTATTAATTAGTTGTGCTTTAACTGCATCAAAATAACTTTGAATTTGTTCTTTTGTTAAAAATCTAATATTATCTTGTTTAAATTCAAAGTTAAAATCCAATGCTTCTGTATATGATGGCATTAAAGATTCAATGAGATTAGTATCATCAACTAATGGCTGAGCACCATTAGCATTATAAACTTTAAAACCATTATAATTTTTAGGATTATGAGAAGCGGTGATCATAATTCCAGCTCCTGCTTGTAATTGATTAATTAAAAATGAAGTAATTGGAGTTGATAACATCCCATAATCTTCATTAATATAAACAGTATAATCAAAACTACTAAAAATTTCAGCAACTAGTTTCAAATTTTCACGTGAGCCAAAGCGGTTGTCTCGGCCAATAACAATAATTTTATTAAAATTCTTTTTCGTTTCTAAATATTCAATAAAACCTAATGTCAATTGAGTATAAGTTATTGCATTTAAATAACGAGTTCCTTCTTCATAAATACAACGTACACCTGATGTACCGAATTTAAATCGATAATTTTCATCAAAAATATGATCAATTTCTTTTTGAGTTTTATTCTTATATTCTTCTTTAATTACTTGAGGAATTTTTGGTGAATTTAATCAACAATTAAAAATTTTATTTGTATTTTCCATAATTTTATCTCCTTTCTTATTTTATAATGAATAACTATAGAGATATATTTGGATCTCATCCAAATGGTAATAATTGTTCAACATTATATTCTTTATAAGTACCATCATTGTTAAAAACGACGATTTTTGCATCCTTTTTAAAAAATTCAAAAATCACTTGACGACATGCACCACAAGGACTCGCAATATCACCATTATTTTTTGTAATTATATAAACTGTTTTAAATGTTTTTGCACCATTTGCAATTGCATTAAAGATTGCATTTCTTTCAGCACAAGAAGTTAATCCAAATGATCCATTTTCAACATTCACACCAGAGATAAATCCTAAATCAGTATCAACATAAGCAGCAACTGGATAATTTGAATAAGGACTATAACTTTTTTTTAATAACTCTAATAATCCCATATAAATATCATTATATTTCATACTTCAATAACTCCAAATGTTACAAATATCTAACACTATATTATAACATTTTGAAAAAAGTTATAACCTTTTAATAAATGATAAGAGTAAAAAAACCATCATTATTGATGGTTTTTATTTTAAATGGCAAGGGTAACAGGACTCGAACCCGCAACACGCAGATTTGGAGTCTGCTGTTCTACCAATTGAACTATACCCTTTTTTTGTAGTTTATTTATATGCTAACCTTGTTAAAACCAGGGCTTGTCCGTCTTTTACTACAATTGTATCTTCAATTCTTACACCACCAACATTTGGGATATAAATTCCTGGTTCAACAGTAACAACTGCATTTAATGGCAATTTATTAGGATTACCAGCATTAGTATTTGGTAATTCATGTACTTGTAAACCTACACCATGACCTGTTCCATGTGTGAAATAGCCCTTAAATTTAGAATTATCAACAATGTCGCGACATACTTTATCAACTTCTTGACCTGTTACTTTTGTACTCAATAGATTAATCCCTGCTGTTTGAGATTCAAGAACTTTATCATAAATTTCTTGCATTTGTGGATTTGCTTTATTTCCAACAATAAAAGATCTTGTAATATCAGAACAATAACCTTTATAAGTACAACCAATGTCAACAGTTACCATATCTCCATCTTCAATTACACGATTTCCTGGATGATGATGTGGTGATCCGCCATTTGGACCTGAAGCAATAATTGGATCAAATGAATTTAATTCTCCACCTAATTCTAACATATGTTTTGAAACTAATGTTGCAATTTCAAGTTCTGTTCGACCAATAATATCTTGTTCTCGAATTCAATTACATGTTAAAGCTGCAATATCAGCAGCTTTTTGTAAATATTCAAGTTCTTTTTCAGTTTTAATAGCACGTAATGTAGCTGATGTAAAAGGAGTAGTATTTTTTACTAATTTTTTTAACATTTCATGAACATCTAATGTTACATAATCAGCTTCAACTAATGCATTAGTAATATTTAATTCAACCATTGCGTCTTTTAGTAGATCAAAAGTTGATTTTTGTGGTGTGCGAGCTAATAAAATTACCTTTGCGTTTTTTACAGTTTCAGACGCTGCTGTATAATAACGAGCATCCACTAGATAAATTGCTTTACCATCTTTATTAATAAACACAAAACCATCACTTGAAGCAAATTCTAAAAATCAATAACGGTTATAATTAGAAAATAAAATCATACCGTCAGCGCCTTGTGCTTCATGTTCTTTAATTGTTTTTAAAACTTGTTGTAATTTGAAATCACTCATAATTTAATACCTATTTTTTCTTAATTTCAACGTGAGTTGTTACTTTTCGACATTTAGGACAAAATTTATTTAAAGAAAGTCTGTCTGGATTATTTTTTGCATTTTTTGTAGTAATGTAATTAATACTTTTACTCTCATTACATTGTAATCTAACGCCTCGTTTAATCGCCATTATGTAGTCACCTCTTCGAACAATAATCTATTAATTATATTAAAAATAAAGCTATTTAACTTATTTTTTTAATCTAATCTTGTTTTCTAACATATTTATAAAATCTGTAATAGAAACTGTTGTCGTTTTTTCACTACCATAGCAACGGTAAGTAACCATTTTGTTGTTTTTAATTTCTTCATCACCAATAACAATTTGATAAGGGATTTTTTGAATTTGAGCTTCACGAATTTTTTTTGATAAACGCTCATTACGTAAATCTACATTACTACGAATTAAATGCTTTCTTAACTTAATATTTAGCTCCTTTACATAATCAGTATGTAAATTTTCATTAACAGGAATGATAACTACTTGAATAGGTGATAATCAAAGCGGTAAAATACCTTTAGTTTGTTCTAATAAAATTGCTAATAAACGTTCATAAGTTCCAATAATACCAATATGAATCATTACCGGTACGCTTTGTTCATTACTTTCATTAATATAAGTTAAATTAAAACGGTTTGGTAATAAAAAATCTAATTGGATAGTTGAAACTGTAATAATTCGATTTAAAACAGTTTTAACCTGAAAATCGATTTTTGGACCATAAAATGCCGCTTCTCCTACCATCTCCTTATAAGGAATATTGTGATCTTTTAACATTTTACGTAACACATTTTGTGATTGTTCTCACATTTGAGGATCATCATAATATTTTTCTTTATCATTAGGATCATTTAAAGATAAATGAATTTCAAATGTTTTAATACCCAATCCTTCTTGAGCTTCTTGAATCATTTTAAAAGCATTAATAACTTCATGTTCAATTTGATCAAAGCGACAAAAAACATGACAATCTTCTAAAATCATCTCACGAACCCGCTCAAAGCCTGTTAATCCACCTGATGATTCATAACGATGTAAAATTGAATGTTCACTTAAACGAATTGGTAAACTTCGATATGAACGAAGTTCATTAGAGTAAACTAATGTATGATGAGGACATGTCATTGGACGAAGTACTAAGATTTCATTATCAATTTGTACTGGTGAAAAAATATTATCTTTATAATGATCTCAGTGTCCACTCGTTTTATATAAATCTATACTACCTAATATAGGCGTAATAACAGTATCAAAATTAAATTGAAATTCAACTTCATTAATAAACTTTTGTACCTGATTTTTAATAATGGTTCCATTAGGTAATCAAATTGGTAATCCTTGGCCTGCTAGATTATTAAAAGTAAATAAGTTTAAATCTTTACCAATTTTACGATGATCACGTTCTTTTCGTTCTTGTAAATCTTTTAAATAAGCGTCTAATTGATCTTGATCACTAAAAGCAACCCCATAAATACGAATTAATTGATCATTATTTGCATCACCTCGTCAATATGAACCTGCAATATTTAATAATTTAATTGCCTTGATCTCTTTTGTGTTTTGTACATGCGGGCCTTTACATAAGTCAAACCATTTACCTGAGTGATAAATTGAAAGTGAATTATTTTTATTTTGTTCAATAATATCTAATTTATATTTATTGTGTTTAAACAATTCCTTTGCTTTTTCATAAGTAACTTGTTCATAATCAAACGTTAAAGCTTGAGTAGTAATTTTCTTCATTTCTTTTTCAATTTTTAATAAATCTAAAGGGGTAATTGAGGTATTTGGATCATTTAGATTAAAATCATAATAAAACCCCTCATCAATCGTTGGCCCAATCGCAAGGCTTAAGTTAGGATAAAATTTTGTTAGTGCCATTGCTAAAAGATGTGCAGCACTGTGATTTAGTTTTTGATCAAACTTATACATAAATACCTCGATAAAATATTTTTGATATTATTTTTATTTACTAAGTATATTATATAACGTTATATTAATATAATAATTCTATAAAATCCATATTTTGCTAAAATATAATTTGTTTTAAACCTATATTAAAATAATTAAGTAAGGACAAGGTTATGAAAAAATCATTTTTAATCATGACAGATTCATCAACGACTTTAGATCGTGAATGAGCTAAAAATAATGATGTTATGATTCTGCCGCTTTCTATTTTACGAAGTGATCATACTTTAATTGTAGATGATGGTATAGAATCAAAGCCAGAGCGAATTTATGAAGACATTGATAATGGATACACCTTTCAAACAAGTTGTACACCATATGGTGTTTTAATTGAAGCTATTGAACAAAAACTACAAGAATATGAAAAAATTATTTTTATTGGCATCTCTAGTGGATTTAGTAGTCAATTCAATAATGCAAAAAATTTAGAGAAAGAATATCAAGACAAATTATTTGTTGTAGACACAGAAGACTTTGGCTATTCCTTAGAACATCTAGTTTACAAAATTAAAGCAATGTTAAGCAATAATATTAGTTTTGGTGATATTTTAAAAATGATTAACAAACACCATGATTATACAAGTAGTTTTTTAGCATGTGAAAATATTACTGGCCTTGTTCGTTCTGGTCGAATCCCTAAAATCATTGGTACTATGTTAAAATTAAGTAAAGTAACACCCATAATTAAAGCTGAATGAAAAAATCACCGTGCGGGAATGGCATTAAATATTCGTTCTGCACCCCATAAAATTCTTGAAAATATTAACCATGTTTTTGATAATCAACTAAATAATCATACAATTGAAAAAGTATGTATTCTACAAGCGGGATTATCAAGTGAAAGAATTGATGAACTAAAAAATGATGTAATTAACCATTTTCATGTTGATAAGGAAAAGATTGTAATTAGATCTGGTCCTCCAATCTTTTTAGTATATGTTTGAAAAGGAGCATTAGGTATTCAAGTGATAGCTAATATTCCAAAAAAACATGTTGAAAAAAAACATTAATAATAGACTTTATACAAATATAATCCACAAGCGGGTGCTAATTTAATACTAGCACCCTTTTTGGGGTATATTAATAATTCATGAATTTTTGCTAAACTTATTTTATTAAAAGCATAATCTAAACAAGAAGCAACGATCATACGAACCATGTTTTTTAAAAAACCATTTGCATTAATATAAATTATTAAGTGTGTATCTCTTTTGATTTTAACTCAATTAATGCGACGTATTGTCATTGTTAATTCACTAGTTGAAAACGATTTAAAATCATGTTCGCCAACAAACAATTCTAATGCTTCTAATATTTTTTGATCATTAATTTTTTGATTAACAAAAAGTTCATAATTATTTCTAAACAAATCAAATTCATAATCATTAATGACATATTGATATGTTTTAGAAGTAGCATTATATTGAGCATGAAATGAATCATCAACCTTTTGCACTTCTAAAATTTGAATATCAGAACTAGTTCGTGAGCAAAGTGCTTGATAAATAATGTGCGAATCATATTTTAACGCACTATGTTTAACACTAAAGGTTTGCTCGTAAGCGTGTACACCTTTATCAGTACGTCCTGAACCAATAATCCTGATATTATTAATTTTAAAAATTGATTTAAAAATTTCTTCTAAATCCCCTTGAACCGTTCTTTTTTGTGGTTGTCTAGCTCATCCATAAAATAAACTACCATCATAACGAAGACTAATTTTATAATTCATAATTATTAATTCCTATTCCAATAAATAAAAAATAACCTGTTTAACAAGTTATTTCATCAAATTACTTAGCAAACAATGCACTTGCTTCAAATACACCAAATGGAGTGAAATAAAATTTAATCACGGCCAATGATATTAAAAATCCAACGAGAATACACAAAATTCCAAATAAAGCATAATCTGCTAGGTTTAATGGAAAAGTACGATAACGTGTTCGTGCATAACGAGGGTTATAAGAACGTGCTTCCATAGCATTTGCTAAATCTTCTGCTTTTTTAAAAGCAATTGAAAATAATGGTACAACTAGTGAAATTAATGATTTTAATTTTTGAATAGGTCCGCCATTATTAAAATCAACACCACGTGAAGCTTGTGCATTTAATATTCTTTTTGATTCATCTAATAATGACGGAATAAAACGTAATGCAATTGAAATCATCATTGAAGCCTCAGCAACAGGCAATCGTAAAATTTTAAAAGGACTTAACAAATCTTCCAAACCATTTGTCAATTCAATAGATGAAGTCGTAGCTGTTAGAATTGATGATAAGGTTATCATTAAAAAAATTTTTATTGATATATAAACAGCTAATTGAATAGCAATAGGACTTAAAGCGAAAGGACTTCGTGTATAAAATAAAGCGGTTTGAGCCTTACCTAATTGATCACCTACACCTGACAAAATTAAACCTTGATATTTAATACCATTGATCTTTCATAAATTCGTTGACATATAATATGATAAATGACTTAATGGCACTTCAAGTTTTCCAGATTTTAAGGTACTATTACCATAAATTGGATAAAAATTATTCAATCGAACATCTTGGTTTAACGCATTAAAAGTACTTTCTAATTTTTTAACAGTTACGTTATTTTCATTTAAAAATTTAGCAACATTATAATCGGGTTTATCAATTAGTTTTTTAATAATCTCAGGACTAATATAATTTTTAATTTCTCCACCTCAAATATTCGAAACTAAATTTTGGGGATGAGTTGATGAAATATCATTATAAAATAAATTAAATGATAAATTTCTATTAATAGGACCATTAATTCAACTATTATTACCTAAAATTACTTTAGCTTGATCTGTGACATTATAAATAGCAATAGGATCTTTATATGTCATTCAATTAATAAGTAGTAGGATAATGAACAACATAACGACGGATTTAAAAACATTTCATAAAGTACGCTTAGGTAATTTAGCTACAAAAAAGACGATTAAAATAAAAATTCCAATAATCATTTGCGCAAAAAAGCCAAGTGGTAAAAAAATCATAGCAATTAATAAAATAAAAGAAATAAATTTAATTGCTGGATTTAAACGATGAATTGGTGAACGTCGAAACACATAAGCATTAGCACTCATTTATTTTTCTCCTTTTTTAAATTCGATGATAGCATCTGCTAACTCTTCAATTGTGCGTGGTTGTTTTTGTTTTAGAATTTCATATTTAAGATCTTTTTTAATCAATTCATTAATAACTGCAATTACACGAGGAACTTGAATTGAAGTTGAATTAATAATGTGTTGATCAAAAAAAATTTCATAAGGTGTCCCTGTTTTAATAATTTCACCTTCATCCATTACAATCACTTCATCAGCAACTTCAAGCACATGTTCCATAGTATGGGTAATAACAAAAACTGTTTTATTATTTGCTTTCGCATCTAAAATAATTTGCATCATTTCGTGTTCACCTGCAGGATCTAAACCAGCTGTTGGTTCATCAAAAACTAAAATATCATTTTGGATTGCTAAAATTCCAGCAATTGCTACTCGGCGTTTTTGGCCTCCAGATAATCCAAAAGGTGAGCGTTCTAAATAATCATCTCCTAAACCTAATTTATTTAAATAAAATTTAGCTCGTTTTTTAGCTTCGCTTTTTTTTACACCTAAATTAACTGGACCAAACATGATGTCTTTTTCAATAGTATCCTTAAACAATTGGTATTCAGGGAATTGGAATACAAAACCAACACGTCTACGTAATTCTTTAAACCGCTTTATTTTTTTTGATAAATGATAGTTCGTCTTAATTTCTTTTTTAATAAATTCAAAACGTTGTAACTCTTCATAATTCATCTTATCATTAATTTCTAATATAACATTTTTATCAACACGAACTACTGCTATTTTAGTGTTTTCTCTTACATAAGGATTTGTAATTAATTCATGATTATTTTTTGTTTTAATAAATTTTAAACTAATTGGTTTTTGTTTAAAATTTGCTTCAAACAAAATCCGTGCTTGTTGAATATTAACTTCATTAGAAAATAAAACTAGAAAGGTCCATTGATCTAGTTGATTCCGTCATAATAAATTAATAATTTTTTTATCATATGAATCAATCACTCCTAATAATTGGCGTTCAAAATCAAAATGATCACCAGAAACAATATCACCGACTTGAACGAAACCATAACGCGAGATCATTAATCCATTAAAATGAGTAACTAGCGTAGATTTACCAGAACCAGAATTTCCAATAATAAAATAGATTTTATTTTTTTTAAAATCATACGAAATATTATTTAATGCGACTAATTGATTTTCTTGTTTTTCATCATAAACAGCATATAAATTTTTTACACGAACACTAATATCATTAGATATTGGTCGTGGTACTTTAAGTTTTCTTTTGAAAAAATAATCCATTAATTTTTGCATATTTTTTCAATTAACTCCTCATTATTCAAAGTTAAATCAATTTTTAACCCCTTTTCCTTTAAGGTTTTTGCTAATTTTAATGTAAATGGAATATCTAATTGCATTTTTAATAAACGTTCTTCATTAGCAAAAATTTCTTGTGGTGTACCTATATATTGAACTTCGCCATTTGACATGACAATAACTTTATCAGCTTTAACTACTTCTTCCATATCATGAGTAATAGAAATAATTGTTTTTTTTGCAACATCACGCAATGAAACCATTAATTCTTTTAATTCATTTTTTCCTTTGGGATCTAACATACTTGTGGATTCATCAAAAATAATCACTTTAGGATTAATTGCTAAAACAGAAGCAATTGCAACTCTTTGTTTTTGACCGCCAGATAGTTCAAGTGATTCTTTTTTTAATAGATCTTCAATTCCCGTTGCAACAGCTGCATCATTAATAATATCTCACATTTTATTTGGCGGAACCTTACGATTTTCTAAACCAAAAGCAATATCATCTTCAGCCGTAATACCTACAAATTGATTATCAGGATTCTGAAAAACAATTCCGATATTATTTCGCAAATATTTTAAATTAGCAGCACTTATCTCAATTCCAAAAAGTTTAATTACACCGCTTTTAGGTTTTAATAATCCTGTTAAAACCTTAGATATCGTTGATTTTCCAGAACCATTATGACCAATAACACAAACATATTCATTGTCATTAATTGTAAATGAAACATTTTTTAAAATCATTCGTTCTTCTGTATAAGCAAAATAAACATTTTCAAATTCAATGGCAACACTTGAATCATTAATAATCTTATCTAATGATGAAATTTGATAATTATGAGTATTTTTATCATTATGACTCATTTAATCAACTCCTTAAAGAAGATATTTATATATTATATTATGTTTTTTAATAAAACATATATAAAGGCAATTAAAAATAGGTAAATAAAAGGTGTTAGATAACTAACACCTTTCACATGTTTTATTATAAATAAAAAATTTAATTATTATTAATAACAACTTTTATTCCAGGACCCATTGTAGGTGAAACAGCAATATTTTGAATATATTGACCCTTTACAGTCGCTGGTTTTTTTGATTTAATTAAGTTTAATAATGCATTAGCATTTTCTTCAATTTTAGTTGTTTCAAATGAAACACGACCAATACTCATATGGATATTTCCATAAGTATCTGTACGGTATTCAATTCGACCTCTTTTAAATTCTTCTACAGCTTTTAGAACATCATTTGTAACTGTTCCAATTTTTGGATTAGGCATTAGTCCACGAGGCCCCAATACTTTTCCTAATTTAGCTAATAATGGCATCATTTTTTGGTTAGTAATAATTACATCAATTTGGTTTAACATCTTTTCAATATTTTGAATGTAATCTGCTCCACCAAAATAATCAGCACCAACTGAAGCAGCCTCTTCTTTGGTAATATCTTCACTTAAAACAAGAACTCGTACATTTTTACCATTACCGTTTGGCAATGAAATTGACCCACGTAATTGTTGTTCAACTTTAGTAGTATCTAAATTCAATTTAATAGCGATATTAATTGATCCATCAAATTTAGTAATTGACTTCTCTTGTGCTAATTTAATTGCATCAAATAATGGGTAAGCTTTTTGCTTATCAATTCCTTCATAAGCAGCAAATAATTTTTTAGAAATTTTAGCCATGATTAGTTACCTTTCTTTTCTTTATATGCACTTACGCCTTCAATTACAATTCCCATTTGTTTTGCAGAACCAGCAATCATTCTTAAAGCGGCTTCTTCATCATATGCTGTCATATCAATCAATTTATAACGAGCTATTTCTAAAGCTTGTTCTTTTGAAATTGTTGCAACAGTTTGCTTTTTAGGATCTTTGGCACCACTTTTGATTTTAGCAATATCTTTTAGTAAAAAAGTCACGGGAGTAGTTTTGATAACATAATCAAATGATTTATCTTTATAAGCTGTAATAATTACTGGGATAACCTTACCGTTTTGGTCTTTAGTTTTATCATTAAATGACTTAGTAAACTCAGCCATATTAATACCAACTGATGCTAATGCTGGACCAGGTTTAGCTTGTCCACCAATTAGATTTAACTTTGCGATTCTTGTAACTTCTTTCTTTTTAGGAGCCACACGCAACACCTCTTTCTACTTAATTTGTTAAGTTAAAATTTAAGATATTCGCTACGTGGTTTAACAAGTTTAAAACTCTTCCACATGCTAATAAATTGTGTAAGTAAGTTACTACACGTATCAAATTATACTATACAACTTACTTAAAATCAATTAAAATTTAAAATTACTATCACTTTATTAATACTTGGTTTTAATGGTGAAGGATGGTATAATAATAAAATTATACTGATTTAAATAAACACAAGTTGTACATTTGTTTTTAGTTTGGAGTTAATATGAAAAAAATGTTTCTATTAATGAAGAATATGATGCGTTTATTCTTTAAAAATAAAACTTTAGTAACTCAACTTTCAATTTTAATGATTGTTTCAACAGTTGTTATTGTTGCAACAACGATTACAGTACAACGTTTGCAAAAATCACAAAGCGATATTAAAAAAATTGGTTTACAAAGTGATTTTTTAATTGATACAAAAGAGCAAGATAATATTAATTTCAACGAAATTGACGTTAGCAACAATCTTGTAAAACATAATGAAAAATTAATTCAATTAGCAACTTTTAATCAAAATATTTTAAAAAATTCAAATAATTATTTACAACTAGTTGATAATGCATTTCATAATCATAACCTTTTTTGGCCAACTAATGTAAACAATCAAAAAATAGATGTACTAAATCTTGAAGGAAATATCGATTGAAATAAAATTAATCAATTAAAATTACAAACTGTTTTTTGATTTGAAAACAATAAAGAAAATAATCAAAATTTTGCTAACAATTATGATGATTTGGTTTATGAATATCATAATTCTAAAAATCAATTAGTAGGATTTCAAAATTTAGATGGTGGGCATATTTTATTTAAAACACCGGCGTTAAAATCAGCAAACGGTAATTATGAAACAACATTTAAAATTAATGCTGCAATTGTCCAAAAAAATAAACCATTTAAATCTTTTTTTAACTTTGATAAATATAAAGAATTAGGATTTAAGAATAAATCTTATTTTACAAATCCTGTATTAATTAAAAATATAGATCTTTATTCAAAACAAGTTGTTCAAACACTAAACGATCGTTCAAATCAATCAATTGATTTTAAAGACTATCAAAGCACATTTCAAGCCATTGTTGATACACTTGAAACTAACACGATAAATAAAGATTTTTATAGTGTGAATTTACATGTTGATTTTAACACACTATCAACAAAATACCTACTATTTGCTAATTATTTAAAAAATGAAGGTATTGATAAAAACCGTTATATAAATGAATTAAAAATCGATAACTATAAACAGAAGTATTTAGATTTATTTCAAAACCAAGTTTTAATTCCAAAAGAATTAATTGACGATCCAAAAAAAATAGAAGAAGTGCAAAAAATTATTGCAAAAGCCAAAGCCTTGTTAATTGCTAAAATTCGTGAAGAAACAAATATTTTTTATGAAAACTCACAAAAATCAGCTCTTGAAAATAAAAATAAGATTCGTGATTATGAGATGCAATTAAAACAAATAAATATTGCTCTACAAAATTCGAATTATTCTAATAACGAACTTATTCAATTAAAAAATGAACAAAAAGTATTAGCAGCAAATCTCGATCAAGCAAAATTTAAACACTTTTTATATAATGATTTAATTAAACAAGATTTCCGTTCGATTTTAAATAATTATAATATTTATTATCGCAAACATGAATCAGCAATTTATAATGATGTTAAAAGTGATTCAAATTTTATTGTCACAAATGCAAGCAATCCAACATATACACAAAACGGTTTAATTGCACCAAGAAATCAATGAATTGATAATATGGTGCAAATTAATGAAAAAAATAAAAATAGTCAAAATCCAAATAAAAAATTTAAGCGAATTTTTATTAATGATGATAATTCAAATAAAATTAATAACTTTTATGACCTTTATTCAAATTACATTAAAGTCATTCAAATTTTAAAAAATAGTTATGAAAAAGATCCAGATACTGATAAAGATTTACTAGTTGAAGAGCGTGATTATACTCCTTGAATTAATACATCACTAATTAATATATATCAACTTCCACAATTTTTTAATATCTTATTTAATCAATCTGAATGATTATTATCAATTCAATATCTAAAAGAAATGAGTGATTATAAAAAATATTTACTACAAATTTTTGAACCATATCAAGATAAAGAATGGATTTTTAATTTAAAACATAAAGATTGACAAGATGGCGAACAAGTGGTTGCTATTTATAAAAATGCAGATAGTAATAGCGACCAAAAAGAATATTATTTTAACGGCTATGTTAAAAATCAACGAGCTTATATTAATGTTAAATATAATAAAAATCTGCCAACACGTTTAAAATTAATTAATATTATTAAACAAAATCAAATAAATCAAAAAAACAATTTAGTTAATGTTTTAGATCAAGATTTAAATGATTATGCAAAGATTATTAATTTTAAAAAAGGTATTAAAGATATTAATACAAACCAGCAATCAGTTTATGATCAGTGAGCTTTAAATAATCCACAAGATTATGCTATGTTTAAAATTTGATTATCAAAAATCATTAAACGTTATTATTTTCTACGCTTCAGCAATCACCTTATGGATATACAATTCAAGCACAAAACGTTTTATCAAAAGCTTTGTTATTGCCGTTTACAATTAATATTATTAATCGTAGTTCAGATCTAGCATACGTAAGCACATCTTATATTAAAGCTAATCAAAGAACAACAAAAAATATAAATAATTATAAAGAAATTGGTAATTATGAAGATTTTTTACAAGCACTAAAACTTCCATATCGTATAATCGATGATCAACCAAACTATCGTGAAATTTACTTTGCCAAACGGTCGTAAAAAAAGAATTGCTAAAGATTATTATAGTTGAATTAATTATGTTGTTAGCCCCGCTAATAAAATTGAAATTAACGGCCAATTCTTCTTTATTATTGGTGCTGTAGATAGTCCTGAATTTTTATTTCCAGCAATAAATGACTCAGACATCTTAATTAATAAACAAAAATCAACTGTTTTATATGTTAATGATTCAGGTTTTGCTAAACTTAAAACTATTTCAGCAAGCGTGCCAATTTTAGAGTATTACACACTAAAAGTTAATACTAATGATCTTAATTTATTACAAAAAAATAATGTTTATAATGAAATAAAATCAGAATTATTAGATTCTAAAAAAGGTAACATATATAAGAGAACTGATAAGGATAACCCTTATCCAATTTATGTTAAACGAACCTTTTTTTTAACACAATTGAGGAATTTAATTATTGCAATTGCACTTAGTTTGATTGTTTTAATCTTATTGTTAGGAATCTACTTTGTTACCTCAAGTATTAAAAATATGATTAATAAAAACAAAGTCATGTTCGGTGCTATGCAAGCTCAAGGCGTATCAAGATGACAAGTTTGATTTTCATTCGCTCCTTTTTATACAATTCCTTCTTTAATTGTTTTAATTATTGGTTATAGTATATCTTATGAAATTCAACCCTTAATTATGCGTTTATTTAGCTCATATTGATTAATTCCTATCCCAGACCAATTTGTTAGTGTTGGTTGAATTTTTGCTATTCCAATTGCTTTAACATTAATTTTAGGCTTTATTTGCTGGGTGTTAATTTATTATATTTTAAAACATTCAACCGCAGAGACAATGAAAGGCGATGCAGGTTTTAAAATCAATAAATTTATCATTAAAACAAAAGCAATCTTTTTAAAATTTGGTGCAATTCGAGTTTTAAAAGCTACTTATGTTGTTGCCAATTTAGCAAGAATGTTGATTTTATTAGTAATCGCTTCCTCTTTTTCTATTATTGCTTCTATTATTGCAACAACAAAAAATAATTTTAGTCAGTCAGCAAGTATTACTAATGCTAAACGTGAATATCAATATGCAGTTGATTTATATTCACCAACAGAACAGGGAGGATTTTATTACATTTCAAAGTTTGATGAAATGGGTGTTTCTAATGAATATGCTCCTTTAAAACAAATTGTTAAAAATGTTAAGATTGTAATTAAAATTAAAAATTTAGTTAATAAAAAAGTCAATGTTGTATATATCAATAATCAAACAAAGCAAAAAGTAATTATTGAAGCACTTATTAATGATAAGGGAATAATCACTTTTAAAACTAATCAGCTTGTTAAAGGTAGTTTTCAATTTGAAGGTATTTATGAACAAGAAACAAATAGAGTTTTAATTAATTCACAAAATTTAAATCCATCATTATTAAATATAGATAATCGAAAGATAGGCCATTTTATTTACTCTAATCAAAAACAATATATCTATTTACTAAAATCATACTTTAATGACAATGATGTAATTTTAGATTATGTTGATGCTAATAATCAACATACTTATTTAAAAGCAAAAACTCTAGGTCAATACTTGGTATTTGATCCTTCACAATTAAAACAAAAACAAAAATATCAATTAGTTAATGTTATAAACCATAAAGGAAATACATGAGTTGATTTAAATAATATAAGTAATGATTTAAAAAATTTGGATTATTCCAAAAATGGACGTTATTTATATGATTATTATGGAAGATTACCATATTTATACTTTAATCATTTACCACAAAATCTAATTAACCAAAAAGTTTATTTGACTTATACAATTAACCAAAATGATGAATTTCGAATTTATGGTATTGTTAATAAAGAAAAAGAAGTAGTTTTTGACTTAACAAAATTAAAAATTAATACCCAACAAAGTTATCAATTACGCGATATGCGAATCGCAAATAGTGATTTATTAATTTATGATAATAAACAACATGAATTTAATAATAATATTTTATATTTTGATAACAATTATTACGGTTTAAACAATAATAATCAAAAGCAAATTAATATTGATTACTTAACTCCTAATCGTAATCTTTATTTAACTTTAAAAGCAATGGGTAATGAATTTGTTAATAAGAAAAAAATACCTTTTATAATGACAAAAGCAATGGCTTCTTTAGTTGAACCTGATACTGCAATTTTAGATTATAATGAATTAGAAAAGGGTTATGTTTATCAAATTAGTACAGCAACACTAGAGGGTAGTGATACAAACTTATACTTAGATAACGTTAATTCTATTGATAAAACAAAAGATAATGGCTTACCTTATTCGTCTCTAAAATACAATAACGCAATGCGTGAAATTAAAAATTTAGGTACTGATCATCCAGTCTATGGCAAAATGTTATCAAATGTTTATTATCCATCAATTAGTACCCAACCTGAAATCTTACAAAATATTCGTTTTTTTGATAGTAAAGTAATTTCAAAAAGTTCTTTAGATGGAAATATTAGTGTTAAAGCAACGGATGTTAACATTGAATTTAACCCTTGAGTTTTTGCAAAAACTTTCTTACCTATCACAGCAATAGCTCGATCAGAACTAAATGAAAAACACTTATTAGATGAAGCATTTAAAACTTTTGGTTATGAAATTATTAATGGTAAAAAAATCAATAAATTATATGATGTTCCTAACTTTGAAGATAATGGTATTATTCATCAAATATCATTATGAACAAAAAATGGTTTACCACCTAAAAGTGAAAATGATGAAAATGCTTTCTTTATAAAACAAAATGGGCAATGGAAAATCAATGAAAAACCAGGCGTGTTAACAGAGCAGGGCGCCGCAGAATTCTTACCAGAATTTTTACGTTTAATTACTCAAATGTACACCTTCAAAACAACTCGTTATTTACAATATAAAGCCGGTTTAAAATTTGTTAATATTGAAGATGAAAAAGTACAACAACCATATACATACTTATTAGGTAAAATGCAAACAAAAACAAAAAGATTTGCTAATGAAATTAAAATATTGGGTATTAATTATGAAAAAAATAAATTTATTAATGGAAAACCAAGTCCTACTACTCAACTAAGTTTGATAAATGATCAGGGTGTTAATTTAATTGATCTAATTAAAAATGATGATCCTAATAATGAGATTCACAACATTATTATTAATAAAGTTGTCGCCCAAAAATATCATTTAAAGATTAATGATGTTTTTGAATATCAAATTAATAATCACATCAAACGTCTATCAAATAAATTTAATAACACACCAGAGAATTATAAAACTAAGTTTAAAGTTGTTGGGATTAATGATAGTTTAATGGATGAGCAATTAATTATTAATCAAAAAGTTGCAAATAAACTAATTGGTTTTGATGATTATCGCCGTCGGATTCAAATCGAAAATGGTTATCCAATCATTGAAGAACGCGAGTTCAATGGATTCTTTACAAAAGAAAAAGAACCTATTTTCTTTAATAATATGGCTTCATTTTATTCACCTACTGGACTATCACCAGCAATTGATTCATGACCACAAAATTTATTAGAAAATATTGGTGGTAATGTTCAAAGCATTAATATTTACTGAATTTTTTATTACAGTTGAGATTTAGCTAATTCGATTTTAAATGCTAGTTTCGATAAAAATAAAATTACTTGAGAACCTTTTTCAGAAATTAATTTAGATAAATTATTAAAGAAAATAGGAAAAATTTTTGGAAAAGAAGCTAATTTACCATCATTTAAAGCAGTTGATGCTAATATTGAAAACGAAATATTTGCTTCAGTAGTTGATAAAACATCATTTACATTATTATTAAATATCATTACTGCTATTATTCCATCACTTGTCATTATTATTATTCTAGTAGCTTCAACACTAGCTAATGAATCACGGCGTTTAATTGCTATTATGAAAGTATTAGGAATGCGTGATTTTAGAAATGTTAATAATTTTATGTTTATTTATCCAATCGTATGATTATTAAATATTCTTATTGCTACACCGCTTTCTTTTGGAATTATTCATATTTATAAATTAGCAATCTTTGCTGCTTTTAATATTGTTTTAGTAGCTTCTATCCCTTGATGAATCTTTGTAATTAGTTATGGTTTTGTCGGGATTGTATTATTATTTGCTTGATTACAAATGTTATATAAAACAAAAAAACTAAATTTACCAAAAGCTTTAAACCAATTTAATAATTAAAAAGACGGCCGTAAAGACCGTCTTTTTTTTAATGTTAATTGCTTGTTTTTATTTTTGTAAGATTTTTTTTAAAACATTAAAAGAAATGGTTGGATTCGCTTGTGCTTTAGTTAATTTCATTATTTCACCCATAAAAAATTTTTCAACACGGTCGGGGCGTTCATTGTATTGTAATAACATTTCTTGATTCTTAGCTAAAATTTGATGTCAAAGTTTAGTAATTTCATTTTCATTAGTAATTTGCTCAAAACCTAATTCCTTAATTAGAATTTGAGGATCTTTATTTGTCAAATAAATTTTTTCTAAAATTATTTTTGTTTGTTTTCCATTAATCAAAGTTTGATCAAATAATAAGATCATTCTAGCAATTTTTTGCAATAATTCTGGTGTAATTTCTTCAATGTTTTGCGTATTTTTATTAATTAGACCAATAAATTCCAAACATAATCATTTATAAACACTTAAAGGATTATTAACAATTTTGTCTACATATTTAAATGCTTTAAACAACGGTCCATCACTTAATAACTGTTCGATAGCAGAACTAACTAAACCTTTTTGTGCTAGTTGTTGACGCAAATCATTAATACTACATGATTTAGTTAAAAACATTTTTTTTACATAATCATCACTTAGATGAATTGGTGCAATATTTGCTTCACGAATATAGCGATAATTAATAGCATCATTTTTTGAACGCATAAAAACCGTTGTATTCGTATTATCATCAAAACGTCGTGTTTGTTGTTCAACATTCTGATTTAGTAAAATTAAATTTAATTGACGTCTTATCTCATAATCAATAGCTTTAGCAACATTTGAAATAGAATTAATATTTTTAATTTCTACTTTTGTGCCAAATTTTTTAGCCCCTTGAGGTCTAATTGAAACGTTTACATCAACACGTAATGATCCATCTTCCATTTTTGCATCTGATATATCATTAAACAATAAAATTTTTCGCAATTCTTCTAAAAATAAAACCGTCTCTTGTGCACTTCGTAAATCTGCTTCACTAACGATTTCAATTAAAGGTCATCCTGCACGGTTATAATCTAAATATATTTGATTACCTATATTAGTTTGTTTGGCTGTATCTTCTTCTAAATGAATTTGTTTGATACGTATTCGTTTTAAATTATTATATTCATCAATGATTTGAATATAACCATTCTGACCTATTGGAAAATAATTTTGTGTAATTTGATATCCTTTAGGTAAGTCTAAATAATAATAATGCTTACGATCAAAAGCAATAAATTGATGATTAGTACGCATGTGTAATGCATTTGCTAAAAAAAGTCCTTTATGAACAACTTCTTGATTAACAGTTGGTAATGTACCTGGCAATGCTAAATCAATTTCATTAATTAAAGTGTTAGGAATACTCTTATGCGATGTTGCTGCATTAGAAAACATTTTGGTTTTTGTATTAAGTGCAGTATGCACTTCAATACCAATAATAACTTCAAAATTTTGCATTATTATTTTTCCTCATTTCCTAGTAAATTATTGATTAAATAAGCGCCATTTAATAATTTATTATCATTTAAGTAATCTGCTCACAAATTTAAACCTAAATTTCCTAATTTAGTTGTTAAAAAGGGGATTACAATTGATGGAAAACCACCAAAATTAGCAATTTGCATTACATCTTCAACTAAATTAGTTGTGGGCTTTTGGTTGTTAACATCATCAATTGTTTCACTTATATCATGAACTGCTAAATTTAAAATTAAATCACAATTTTTAAATAAATTTTTAGCTTGATCAACTATTAATGTACGCATTTTTTTAGCAGCAACTAATAAAGTTTCAAACTGGCTTTCTTGAGTAACATAAGCACCTATCATAAAACGTTGTTTTAATTGTGTTCCTAAATATAAAGTTCGCGTTTTAATAATTAAATCCTCATAGTTATCATAATCAATATCATGTTTGCCAAAAGTGATGTTTGTAAAATTAGAGTATGAACTATAAGCCTCACTAAAAGCGATTAATTTATAAACTGGACTAATTGCCTTTAATAAATTAATATCATAATCAACATTAATTATTTCATGCTCATCATTTAATTTAACAATCAGATTAGTTAATTCTTGTTGATATTTTGATGCTAATAAATCAAAACTAGGTTTTAAAACACCAATCTTATATTTATTTTTTGTTTTTAGCATTGAAAGTGATTGTGTTTTAAAACTAAGACTTGTAAAATCTTTAAAATCTCGCTTAGCAAGAGCATTAAAAACAATTTGCGTATCAAAAATATTATTTGTAAAAATTCCAACATGATCAAGAGAAGGAGCAAAGGGGTAAACGCCATAACGCGAAATAAGACCATATGACGGTTTAAAACCAACAATACCCATAATTGAAGCGGGGCGACGAACAGAATCTCCTGTATCTGTAGCAATTGCAAAAGGTACAACGCCTTGTTGAACTAAAACAGCACTACCAGATGAAGAACCTCCGCTAACATGTTTTGGATTTAATGGATTAATTACAGGGCCAAATGCTGAATCAGTACCAGTGCCTCCTAAACCAAATTCATCTAAATTTGATTTATTTAACAAAATTGCTTGTTTTTGATCTAATAATGTTTTAACTGTCGCATCATATGGAGGATTGTAATTTTTTAAAAACAATGAACCACCTGTTGTAATAATATTCTTAGTTGAAATGTTGTCTTTTAAAGAATATGGAATTCCAAATAAATAATCAATTTCTAAATTATCACTACTAATCTTTTCATCTAAAAAAGCTGCTTGTTTCAATGCCTGTTCATAATTATTTGCTAAAGTTGCATTTAAATTTTTAATCTTTAAATCATGATCAATCGATTTTTTAATCAAATCATAAATTTTAACTTCACGTTTTTTAATTTGATTATGAAGCGCTAAAATAGTTTTATTTTTCATTTTTTAATACCCCATAATGATCGATTACTTTTAGGCAATTATTTAGAACTTCTTCGCTATTATTACCTACACAAGAATTAATTTCATCTTCTCGTAAAAAATTATCAAAGGATTTATTAATAATTTCAAAAGGTGTTATATTTTGTAAATTAAAAAGCTCAAAAACTTTAATTTCATTAAATAAATTATTTAAACGATTTTCAACCTGTTTAATTTCATCATCAGTTAATATAAACATGCATGATGACATTAATTTTTGTAAATTATAACTCATCTTAATTTCTCTTTTCTTTATTAGTCAACATGAATTGGAATATGTTTATCCTCAACAATATCATCTTCATAATCATAACGAATTAAAGGAACAACAATTTTAAAAATTGGATAAATAATCAATAAGTTTAAAACCACCATTGGAATAAATAAAACAGCACGAATTGATAATCATTGTGTATATTTTAGACTTGATAATTCAGCATCAAACGATGGCATCATTAGTACATTAACCACTGCTTCAGTTAGTAAAATTGTTACTAAAACAGGGGCAAATATAATGAATCAATTTGTTTTGGTTTTAGCTTTATTTTTTTTATTGAATCAATATTTTATCAATAAACTAAACCAAACAACGATAATTGATAATAAGAAAAAACCTAAAATAATGCCAATCATCAATGTTCGTGCAATTTTAATATCAAAACCAAATAATTGAATATTAAAACCCTTATCACCAATTCCTGGAGCAAAATATAAAAATAAGGCAATTCCTACACCAATTAAAATAGTTGCCATTGAACTATAAATTGCAAATGGTAAATCCTTTTTTTTGGAAGTAGTTAGAATAATTCGAATTAGTCCTCCAATTAATCCATAAGCCATAGCAGCAATTAAATAACCATAATGAAATACACCAGCAGTCATAGCCACAGATAATAAATCAGTCATTGCACCAATAAAAATACCTAAAATTGGACCAAATAATAAACCTCCAATTTTAATAAGAATCCCTTCTAAAGTAACACGTGTACCTGGTCAAGCACGAAAAATTACAGAAGCTGTGTTAGCTGATAAAACTGTTAATAATATAATCACTGCAACAGAAATCGAAATCACCATAGCAATATTTGAAATCCCTTTAACCGAAGTTTTAGGAATAATATAAAAACGCTTTTGAAAACGAATTGAATAATAGATTTTTTTAATAACTGTAAAGATAATAAAAACAATCAAAAATGCTAAAAAAATTAAAATTGATTGTCATGAATTAATTCCATTACCTTGTGAAGTATTAGTTGTATGTAATACACTCATTATATTTGTCACAGATTCACCTCACTAATTAATAAATTTTATCATTTTATTGGAGTGCGTAAATGTTTAATTAAATAATCATTGCATTTACTAAAATGTTTACAAGTTAAAAAACCACGATGTGCACTTAATGGCGAAGGATGTGAAGCACATAAAATTAAATTTTGTTTTTGATCAATCAAATTAATGTAACTCATAGCTTGTTTTCCTCATAATAAATAAACAACATGCTTTTGTTTTCTAAGTTCGTTAAACACATTTTTAATTAATTCTTCATAACCAAAATTTTTATGCGAATTTGGTTGATGAGCGTTAACAGTTAAAACAGTATTCAACAATAAAACTCCTTGTTTTGCCCAATCAGACAAATCAGGGTTTGTTCTTTTTATTTGTAAATCATATTCTAAAGCTTTAAAAATATTGATTAGTGATCCTGGTAAATTATTTCCTAAATCCACACTAAAACACAAACCATTAGCTATTTTAGGTGTATGGTATGGATCTTGCCCTAAAATAACAACTTTAGTTTGTTCAATATCAAAAAAATCAAAACATCTAAACCGTTGTTTTTTTAAAGGAAAAACTACTTGATGATTCTCGATGTTGTTGATTTTTTTAATAATATTTTTTAAATAACTTTGTTTAGTTTCATTAATTATGAATTCTTTTCATTTCATTTGCTATAGTTAACATTTACACTTTTAATTTCATTAAGGTTAAAAAGATATTTTCTAAAATTATTCTTAGCAGCTTTAAAACTAGTCAAATTATGAATATTAGCATCCTTATTTTCTAAAGCAATCATTGCTAATTGCGAATATGATTGTTTTGATAAAATTTGGTTGTTTTCAGGGTTAGTAACAGGTAAAATAGTACGAGAATTGTTAGATGCTTTAATTGCTTCAACAAATTTGCGTGATTTGATGAAATCATCTATTTTTAATTCATTGATCTTTTTTTCACCTAAGAAAGCATATGGATAAACACCATTTAGACCACGATCATGTTCATAATATTGTCAGTCTTCAAGTTTTTTATCTTCAACATACTCTTTTTTATTAACTTTAGCTTCGTTTTTTAATTCTTGTTTTAAATCGCGGCCCAAAATACTAAACGCATGAATTTTAGATAATGTACCTGTGTAGTATTCATTATAAACAGTTGTACGTTTTTTCTCTGTCTCAGTTAACCCTGTTTTTTGATTTTTAATTTTTAAAAAATTTTCTTGAAGAGTTTGTGTTGCTTTTTGATTAATATTAGCAGAACGCATTGTTGATGGTGCAAAATTAACTACCATATCTTTTAATGGAATTTCATAAACTAAATTACGAATTTCTGGTTTGTGATCATCATGAATTTCAGCATAAGTTTTTTTATCAACACTTGATAAAGCAGCGTTAATTGAACTAAGTGCATTTAGTTGACTACCATTAGAACTAGCTTCCATAAATGATCCAGCTTTTTTGTTTGAAGAGTTATATCAAGCATATTGTAATTTAATATTTTTTAAACGTACAAGAACATTTTTTAAACTTGGATTAACTAATAATTCATCATTTACTTTAACACCAAAATTAGGTTTATTAATACTTAAACCTGTAATTGCATCTACATATTCTCAATCAAAACTAAAATTATATAAAGCAAGTTTGTATGTATGTTTACCTGCACCTACACCTTGTACTAAAGCTAATAAAAATTCTTTAGCAACTTCGTTTTTTAACCCTTTAAAAAGTTCAAAATCTTTATTTGAATGGTTATTAAATGCATGTTCTGCTAATCATTTTATTAATTGATCTGTCATAGAGGTAATATAAGTATACAATCCATCACTAATAACTAAAGAGTTGATTAATTCACTAACCTTATTTATTTTTGTTAATTCATCACTTGAATATAGTTTTTGGTTAAAAACAGTTTGTTTTTTTGTTTGGTCAAGAATTTCTAAATTATTAGCATATTTACTTGTTGAATCAAAATTTAACTTATTACCAATTAAACGAATCAATTCATTTTGCAAAATACTCAAATCAATTGGTGTTAGTTTAGTTGAACTTGATAAACTTTTTGTTCAAACTTCTTTTTTGGGAACCAAATAACCAAATTGATCGATTTCTTTATTGTTTTTATCAAAGCGTTTATTATCTTTAACACTCGTATAAGTTAAATCACCTGCCGCATAATAAACATCATCTAATAAATTTTGATTCTCAAAAATCATTTCATGTTTTTGATTCTTACCTTCAAAGGCTCGAATTTTAATTTTACCAATATTATTAGTTGTAGCATTAGCACTAACATAATGAACATTAACTTGAGGATGAGCAGCTGCTGAACACGATGCAGCAACAATTGGCGTTAATCCAATAAAACTAATTCCTAATATTGAAAAAATTAGCTTTTTACTTTTGTTTTTTTTCATATTTACTCCTTTGAAGTGGTTGCACGAATAAATTTACTATTTAATTTAGAAAAAATAATATCAGTTTCACCTGTTGACCCGTTTCGGTTCTTTTCAACAATAAATTTTGCTTCAATCAAATCATCGCGCATTCGGGTTTCTTGATTATCATTTTGCATAAAATTGCGTTGTTTTCGATCCCAATGTAAAAAAGCAACAACGTCAGCATCTTGTTCAATTGATCCAGATTCTCGCAAATCAGACAATTTTGGGGAATTATCAATTGTTTTTACTAAAGGGTTATTTTTCATATTAACATTATTGCTTACATCACGTTCTTCTGCTTTACGTGAAAGTTGAGCGATAGCGATTACTGGTGCATTAATTTCTTTAGCAATGATTTTTAATGCACTTGAAACACGACCTACCTCTTGTGTTCTTGTCATATTTTTATTTGCATTGCCTCCTGAAACCAATTGTAAATAATCTAAAACGACTAAAGCAATATCATAACGTTTTTTTAAATCATATAATTTTGCTTCAATGTCTACAATTGAAATATTAGGACGATCATCGATAAAAATAGGTCAACTTTTAATATCATTGATTGCTTGCATAGCAAAAGACCTTGTGTCATATCCTTTAGTTATACGATATTTATTTTCTATAACTTCACGATTAGAAATTCCTGTTTTAATTGAGATAAACTTTTGTAAAATTTGTTCTTTAGTAATTTCAAGCGAAAACATTACAATGATCTTTTCTTTTTGATTTGGTTGAATATTTTCGTTATATTCAATAATTTCATTAACATTGTTAACCATAACATTTAAACAAAATGTAGTTTTTCCAATTCCAGGACGAGCGGCAATAACAACTAATTCTCCAGGTTTATAACCATTAGTGAATTTATCAATATTATCGTATTTAGTTTTAATAACTCCAGGAATAATCCCGCTATTGCCTATTTTATTTAATTTCTCAAAATATCGATTAGCAATTAATTCCATATTTTCAATAGTATCTTCGGTTCGTGATGCAAGAATTTCTAAAAATTTTTCATGCATTTCTTTAAATTGATCTTTAGCGTTAATTAATGAAATTTGGGTTGCTAAAATCTCATTTGCAAAAACATCAAGTTTATTTTTAATTGAAGCATTCTTAATAATTTCAATACAATCTTTTAAATCTACTAAATAATCAGTACGATCTAAAATTTGAAGTAAATAATTCTTATGTAATAAATAATTTTGATCTTGTTCATCAATAATCACATAATTAGCTAATGCATTTAAAATATTTGATTCATTGATTTTATACTTTTGTTCGTTTAAATTAGCAATTGTATTAAAAATATTTTGCTGAATCTTTTCTGGAAAATCTTTAACTTCTAAACGTAAAACAACATCTTTTCAAAAGTCATTATTATTAATAATTAAACAAAACAAATCTAATATCGCATCATTTAAGTCTTTTGTTGGCATAATTATTATTCTTTGCTAACAATAACATGAAGTTTAGCAGTAACCTTTTTATGCAATAAAATTTCAACAATATGTTCACCAATACCTAATGCATGTGGTTTTGTTATCATATGCTTTGTTATAAATTTTTGTTCTTTATTAATTTCATCAATAATTTGTTTATTACTAATTGATCCAAAAGTTTGTAAATTATTTGTTTTTAAAAAAAATTGTAGAGGCTTTTGCTCTAGTTTATCTTTTAGTAAAGTTGCGTCTAAGATTGCTTGTTGTTCTTGAGCTTCTAAAATTGCAAGATCTTTATTTAAAACTTCTTGTGATTTATTTGTTAATGCAACTGCTTTTTTTTGTCGAATTAAAAAATTTTTTGCATAACCATCACTAACATCAATAATTTCATTTTTTTTACCAAGACTTGCAATATCTTCTAATAAAATAACTTTCATAACTTTACTCCTTATTATTTAAATAAAACGAACATATCATTAATTATTTATAAAATGACACATTCGTTTTATTTTGATTTTAATTATTCTTTAACGTATGGAAGTAATCCTACGAAACGTGCACGTTTAATTGCATTTGCAATTCTTCTTTGCATACGAGCACTTGCTCCTGTTACACGTCGTGATGCAATTTTGTTATTATTGTTAATAAAACGTTGTAGTAATTCTACATCTTTGTAATCGACATGTTCGATTCCTTTAGCAGATAAAATACAAACTTTTTTACGTGGTTTACGATTACGGTTATTAGTTACTTTGGCCATAAAATCTCCTATTCTAAATCATCATCAAGATTAATTCAACTTGTAATTTCATCTTCTTCATCATTATTTTTATTTAAATTTGATGTTGTCGATGAAAGGGTAGTTTGAGGTTTGTTTGAACTAAATGCTGCATCAGTATTAATACCTGTATATGATTCCATCATTGTATCAACATTTACCTTATTGTTATCACTAGGACTTTGGTTTCGACGTGATAATGATTGAACTTGATCAGCGTAAACTTCAGTTACATAATTTGTTTTACCATTATTGTCGACATAACTTCGAGAAACTATACGTCCTTCAATTGCAATGGCATCACCCTTTTTTAAATATGTTGTTAAGAAATTAGCCTGATTATTTCAAGCAACACAACGTATAAAATTTGCATTAGCATTAGGTGTATTGTCATTTACAGCGATTGTAAAGTTAGTAACTAAACGCCCACTTGGTATTTGACGAGCTTCTGGATCACGTACTAAATTACCAATCAATATTACTTTATTCATTTTACGCCTCAATATCTTTAAAGTTAAATTAAAATACGTTATTTTTAACGTTTTGAATTTGTGTTTTTAACAAATTCTTTAACTGGCAAACCAGCTGCTTCTCGTTCGGCTTTCATTGCTTCATAAGCTGCTTGACGTTCTACTGCACGACGTTCTGCTTCTTCTTTTTGACGTGCATAAACTTCAGCACGTTTTTCATTACGTTGTACTTTTTTAGTATTAACAGTTGCTTTATAACCGTAATCTTTTTCTAAATTAATAATAATATGTCTCAATACATTTTTATTAATAATTGCTAAACGTCTAAATTCATTAATTAGGCTAACATCAGTAGTTTCAAAATTATAAACATAACGATAAGCTGTTTTTAACTTATTAATCTCATAAGCTAATTGTTGCACGCCATCATAATTATTTTCTTTTACTTCTGTATTCTTTAAAGTTGCTTTTAATTCATTTGCAACTTTATTAGCTTGTTCTTGATCTAAATCACCACGAACAACTAACATAATTTCGTATTTCGCCATTTATTTTACTCCTCTTATGGATATTAATAGCTAATTTTGTTGTTTTAAAATTAGCAAGAGTGTTTAGTTCAATAATTATTGTTGAATAAACGTTAAAATTATAGCAAATTTAGCAGTATAATAAAGTTAAATTATTAAGTATGGCATAGGATTTTTAGATTTTAAATTATGGTTTTGATAGAAAAAATCAATGAACAAGATATTAATACCGTCGTAGCATATGTTGTTGCTTTCCGTAAAAAACTTTATCCCAAAGTTGATCATAATAACTTACCTAAGGAACTAGTTAATTTTAAACAAACTTACATTATTAATCCTTTAGGAATATGGTTTAGTGTTTTTTTAGAAGATACACATGAATTAGTTGGAACAATTTCATGTCATGAATACGATTATAGATATAATAATCTATTCTATCTAGATAGAAGTACTAAAACAAGTGAGGTTGGTAAATTATATATTGATCCTTTGATTCGACGTCAAGGAATTGCAACTCGTTTGTTTAACGCATTAAAAGACCAAGCTAAAAAACAAGGAATTAAATGTTTTTATTTACACACACACCATCACTTGCCAGGCGCTAGAGAATTTTGGTTAAAAATGGGATTTAAAATTCAAAAAGAAATGACTTTAGAAGATGATGGTCAAGACATTATTCATATGACTTTAGAATTATAATAATATAAAAAAATCATGGAATTATTAACCATGATTTTTGGTCACAACTTATTGAATTGCACAACAAATTTTAATAATAATTAAATTTCTACTCGAAATTGTGATGGTGGAGATGAGGGGAGTCGAACCCCTTTCCGCAACACATAATTAATAATATCTACAGTTTAGTTTTATTAAAAATAGTGTAGTTAATAACTAATAAAACAAATTTAAAATTAACTAACTATTTGTTAAATCTATTTAAATTTAAACAACAAATAAGAATTTTAAATTTAAAACTATTGTAAAATAAGCCCAAAAACCAACCAATAGTAATTATGTTAATGAACGTTTCTACTAATAACTAATTAGTACGCAAAAGCGTAGTTTGCATTAGTAGCTGAAGCCATAAACGCTGGGTTTAATTCTACTTCAGAAGATTTTTTATTTTCTGCATTTAAAATGCCTCGAAAGCGATAAGGGGCTAACCCCACTGCAATTAAAAATTAATGTGTCACGTCGAAACCGTGACATCCCCAAATAAAATAGCACTATAAATAATTTATAGTTAATAATATTTTAAAGGATTAAAAAACAAAAAACAAGATTATTAATGTTTGTAAACATTTTTATCTTGTTTTTTGTAGTTCATGGTTATATTATTTATGTTTATTCAATAGTATTTTTTAAAAAATTATCCACTTCATTTTTTGACACAAATATTCTAGTTTAGAATATCAAAATTATTTAGAAGTTTAACTTTATTTTGAAAAATAAAGTTAAAAATACTATAGTATTTATGTTTTATTTATTTAGAAAAACAGGGTTTTGTGCTAAAAGCGCAACTTCACCCTGTTTTTATTATTTTAATAAGCCGAGATATATTCTTCTTGTAAAGTGTGACTTTTTTTTCGATTTCAATTAAATACTTTATCATACACAACTGTTAATAAAATTGATGAGACATAGTGTATGAAAGGAATTAATGAAATAATTAAAGCTTTTTTAACTTTCGTTTTATTTTCATTAAAATCATTTAAATACTTAATTAAAATGCGAATATTAACAACTAAGCTAATTAAATATGTTAATGTTCATAAAACTAAAAATAGGCCAAAAAAACCAATTGATAAACGAATTTGTTCTTTTTGTGATAAATAAACTTCTAAATCTTTAGGATTTAACATGTTAATTAATTCTTTTTCATGATTTAGGGTATATGCATCAAATAAATTATGACGCACTAATTCATGACCAATTCAAGCTCCTACACAAACAAATAACGCAGCTGCAAATAAAAGTAAAACTCAATTTTGAATTGAAATAAACTTTAATCTTTTAAAAATTAAATCCATAATCTTTTTTAATTATCCCCTTCTGCTTGTGGTGATGTTGGTAAACCAGGACGTGGAGGTCTTCGATAATCATAAGCAAATTGTAAATAATATGTAAAGTTATTTTTCATACCACCAAGTGATGAAATCGTTCAGTTTGTATCAATTTCCATAATAGGCACAATTGCTGCTTCATCACGTAAAATTTTTTCTAAATTTAAAATTGTTCTAAAGATACTATCATCATCTTCTCAACCTGGACGATTCCCTGAAAAGAAATCAATAATTCTTTTACGTTGATCAACAACTGTTTCTAATTTTGTCATATAAATTTCATTATTAACTTTATCAGATATTTCATTATTCTCATTTCTTAAGTACTTACTTAATAATGGTTTTAAATCATTTATGTAAGATAAGTTGATTACTTGATATGATCCAATTGGTAATTTTTTAATAGAAAATTTGTATTTAAATCGTTTTTTGAAATTAATTTCATCAATATTTTCTTTATTATAATATTTTGCATAATCTTCTTTTGTAGCTTGAACATATTGATTTGTTTTTTTATCGATAACTGGTTGAATTTGTAGCGTTGTAAATTGACCTGTTACATTATTTTGCAATACTAGTAAAAACGGTTTATTTTCACGTTTTGGATCGTGTTTATTTAAGAACTCTTCTGATAAATAAAATTCACGTTCTTGCATTGTATCAATTTCGTTCTGTAGATTATTAGCTTCTTTTTCTCACGTTGATTTCGAACTACTTGTAGGCTCATTTTTAATTTTTTGATTTAATTTATTAATTTGGTGTTCTAAATCATGAATGATCTTAGCGTCATATTCAGAATAATTAACATCAAACATTGGAATGGCTTTATCTTTTAATTTTAATTTACGTAAATCAGCTCTATTTGTTAGGTGTTGTGGATTATAAACTTCACTTGTTTTAATATTTGTTGGCGTTACCAATTCACGAATAATATCAGCGTCTTCTTCACTAATATGTAAACGTTTCAAAGCGTAATCTAATTCTGCTTTATTTTTATAACGATCAAAAAAGTTTTTTGGCGTTCAAGATCCTGTTGGATTTAATTCATAACCTGAGCTTTTATTATTTTTTAAATCAATTCCATCAGGTGTTAAAAATAATCCTAATGCTGAGTCATAAGTACTACCAGCATATTTATCAAAGTTTTGATAAGAAATATCAAATTTACCACTATTTGCAAATGTCACAAATGTGTTTGGCGGTAATGCTTGTAACTCTAATTTTACATAACCATTCAGATTACGGTCAATTAAATCTTTAATAGCAATTACAGCTTTTCCAATATCAGCTGGTTCGGTAGGATAAACAAATTTTAAAACAACTTGTTTTTTATCTGGATGTTTTTTCTTATATTTATCTAAATAAAATTTAGCAACTTGTGGATCAAAACCAGTATCTTTTCGGTTAATATTTTCAAATGTTGAATTTTTAGCAGCAACGTGGTCACTAAAATCATTATTTTGGATTAAAAAAGAACGGTTTTGTTTTCTTGTTTCATGTGTTTTTTCATCTTTAATAGTATCAATGTATTCACTATCAAAACGTTTATTATCAAATCAATATTCTAAATTACGCCCTTTTGAATCTTTAATTCTTTGAAATGATGTTCAAGTTGTAACTGGAAAAGATGAAGTTCAACCAGATAATTTTAATATGTTTTCTCTGTTAATAGCAAAAGCAATTGCACGTCGTAAATCAGGATCTAGGATTGGAATGTCCTTATTAGGATTTTTTAAATATTCTTGATAACTATCATAACGTGATTGACGATCTAAATTAAATTGCATTGCAACCGTTCCAGCCCCTGCTTGTTTTTTCATTAAATCACGCATTTTACTTTGCGCTCAGAATTTTAATTGATATGATGGTGGAACAGTAGTTGATGAAATCATCCCTTGTTCAAATCAAAGTGATGTAACTTCATTTTTAGAATTAAAATAAATTTTAATCTTATCGCTAATTGTTCAATTAGCTGAAAAATAATCTTTATTTTTTTCTAATGTCATATAACCTTGAGGTCCTAAAACAACATCAGCAATATTAAATGGACCGCTTCAAACAAAATTTTCAAGAGTAGAACCGAATTTTTCAATTCCACCATGTGTTTCAATAAAACGACGATTAACTGGTAGTAAAGCTTGGTTTCCTAAAATATCAGTCAATAAAACACTAAATGAAGGTTTTGGAGCATAAGTCTCAAATTCCATTAGTAAATCAAAACGTGAAGTTGGTAATGATTTAAATTTAAATAAATCTTTTGAATTAGCTAAAGTTTGTAAATCATCACTTGCCAACTTTTGACGATGATTAAACGAAGGGTTTTCAATTAAATTAATAACAATTTTTTCAGGTGTGTTTTTATCTGGTTTTTTATGATCATATTTAGGATTAAAAATTGTTAAAGTTTTTGACTCATGAAATTTGAAATCCTTATTTTCAGGTAATTTTAAAGCTTCTTTTAATTCTTTATTTGAAATATTAGTATAGGCTTGACCTGTACTTAATCCAATTTCATAAGCTGCTTTTTTAATTGCCGCAATTTCTAGATCATCAACAGGGTGACCATTTTTATCAAAAGTTTGGTTTTGGTATGGAACAAAATTAGGATCTTCTTCTCATTGACCTGTTTGCTTATTTAAAATATATGGAGCACGACCAAAAGGGTTTTTATAAGGAACGCCATGTTTACGAATATATTCTTCTTGAGCATCATTAAAAGCGTTTGTTCCTTTGATGTTAAAACGTAATAAGTGTGAACGTAATTGACTACCAGTATTTAAGTCTAAAATATACTGAATTGCATCAATGAAATCTTGTGCATTAACTTCTTTACCATCACTTCATTTCGAAGCGCCAAAATTAGTTGTAAATTTTGTTGCAACAATACGATTATTTGAATTGTTATAAAAAACTCATGTTGGATCATCTTCACTAACTGTTGGTGCTAACGCTCCAGAATTAATTCCAAAAGATGATAAATCAACATATGATGGTGATATTCTCATACCTTCTAATTGTGCATCAGTCATATTATTAAAACCTGCATCAACTGGATTTTTATATTGATTTAAAAACAACTTTGATATTCCTAATAAGTTACCGATTGTTGAATTTGTTGATGGCGCTTCTTTTATTAAACCATCTACTAGTGAAAATACAATTTTACGAAGCGATGCAAATTTTACATAATTCAATGTGTTGATTGCATCCATTGCCAAACCAAAATTACTACCATCTTCTAAATATGGACTAATTTTATTTTGTTCTTTAGTGGTCTTAGTTCTAGCACAAGAAGCAATAATCGCAGCAACGGGAATTAAACCAACAATTCCAATAGATAAAAATATAATTTTCTTATTTATTTTTTTCATTTTAAACTCCTTAATTTACTAAACTGAGAATTGGTTTGAAATTCTAATAATTGTACGATTTTTATCTTTTGTATTTTCGTTTCTTGCATAAACTGGTGAAACAGTATATGATTTACCGTTTTCAGCAACATATTTGCGTGAACCTAAAGTCATTAAACTTTTTGGGTTTTTTGGATCACTTAAATCCATAACTTCTTTTAGATCTTTATCCACAAAATATATTTCAAATTCACTTCCTTTGGCATTAATTGAATCATAACCAATTAATTGGTTAGTGAAATTAGATAAAATTGCATAATCACTTGTTCATGAAACATAACCTTCATCTTCTAATGTTCATTTAGAATTAATGTCTGATTGACGTTTTAGATAAAATAAATTATTTGTATTATCTGTGTGAACATCGATATATTCTATTTTGCCATTATTTTTATTTTTTATTGCAATTTTTTTAACTTTATCCTTATATTCTTTTTTAAAGAAACCATACATTAAAATAGAATCTTTTTCTTTATTTCGATAAATTTGAGCTAATGTTCGATCACCAATACCTTTTGATCTTAATATATATAGTCACATTGCTCGAGCACGATCTGTTACTTTTGTTTTATTATCTAATTCCAAAATATTTAAATGATCATCTTTAATTGGTTCACGATTGTCATCATAAATCTCTCAATCAATAATTTTTCTTAATCAACGGTCTTTGAAAAAACCATTATTTGTTAATCTTGTTTTACCGATATATGAATTAGATTTAGGACTTAAACGTGTTAGAAAAGAATATGAATTCAAATTATTTAAAACATCACTATCATGAGTTTTTGTCAATGCTAAAAAGTTAGCATTAGATAAAAAGCTTTCCTTAACTTTATTAAAATTACTTTCTAATTCTTGTGGTTTTGTTAGATAATCATCAAATAACTGTTGATTTTTTTGATAAACATTTAAAGCCTGATTTGCTAAAACATTTTGAATTTTTTCGTAATTATTTGTTGCAAATAAGGTATTTAATGCGATTGTATTTGATACACTTAAGAAATTATTTACTGGAATTAAACGCTCGTTTCATTGTTTTGTATTATCACCACTAAAGACATATTCATTCCCTGTATTAAAATCACTTAAACCTGAAATATAATTTGGTAGGTTATCTAACTCATTTTGTGAAGGAATAAATACAGTTTGTACATAATCACGTGTTAATACCTCAGCAATATTAAATACATAATCCCCAAAAATTTCGTTTAATTTTTCTTGAACACGTTTTTTATTTAATGTTACATAATCTGATGAAACAACAGTTCCATCAGCATTTGGAATTTCAGACGATTGAGAAATCCCAAAAAATGATCCTAATCAACCATAAATATCTTTTGAACCACCAGGTATATCAGTATAAATTGTAAATCCAAAAGCATATTGTAATAATTGACCAAAGTTTAAAGTGTTATTCTCACGGTGTTTTCGTTCAATTTCAAGTTGAATTCCTTTAATAATTGAATCAACAGTACGTACATTTTTTTGGTTTGGTTTTGCTTTTCAAACATTCTGTGGATTACCAGCAAAATTATATGTATCAAGTGGCTCTCCATATGGTGAGCCTGGTCTTTCATATTTATTACCAAAACTTCTAAATCCATATAAACCTAGTTTGCCATCAAACACTTGTTTGTATTTATCTAATTCAGATGGGTTAAAATCTTTTGATTTATAAAATTTAAAAATATCATTAGATTCTGTAAATCCATCAAATAATAAACCTGTCAGATTTTGTTTAAACTCTTCTAGTGTTAGATTTTTATATGCTTCTTGTTTTTTTATTACTTCATTGAAAAATTTATCAACATCATAACGTTCTTTTAAATATTCATAATCTCATTTACGAACATTTTCATGACTTGAGCTTAAAGCATTTTCCACAATTGTATATTTAGCTGGATCCACAGACATAAATTCAATTAAAGAAGGAATATCTTTAAATTTAATTGTGTAGAATTTTGATCCATTATTTTCAAGTGTAAGAGGAGTGAATGATCCATTATTTGATTTTAGATAATCAAAATTAATTGCTTGTGCTTCAGCAGGCGTTAAAATCATTAATGGGTAAATTTCTTTTTCTGTTAATTCGTCAAGCTTTTTTAAATGTTTATTATCTTTTCCATCACCTCTATCAACAATCAACTTAATAGTTCCAAATTCATTTAAATTTATCATGCTTTTATTAAATAATTGTTCATATGAAGTAATTTTATTTTGTGTAAGATAGCTAGGACGGATACCAATAATTGATTCAACTGATTCTTTTGTTTTTGTTCTTAAAATATTTTGATATAGTTCTTTTTCTTTTGGTTTAACAAAATATTTATAACCATTATTACCTTCAATTCCAGCGTCAAAATAATTAAATAATGTTGTTTGAGCAACTACATTAACATTTTTAACACCGAAACGATCAAACTGTTTGTCATAATATTTTTTAATTTCTATTAATTCTGGATCAAGTTTGCTGACTTCTTCTTTAGATTTATTATCTCAAATTTTTGATGCATTGTATTGTAAAGTTAATAAAGCATATTTAAATTGGTCAAAGGTTTTATTTGTAATATCTTCGCCAATAATTTGCGAAAGAACTTTTTTATAATATGGAACAGATGATGCATTCGCTTTTTCATCTAATTTTAAAGATTTGCTAATGTTAAAATCAGGGTATTTAAACATCGCATCAGTAATTCGTTCAGCTCATTTTCGTTTTTCATTTGAAGTAAACATTGGGTGATCAAAATATGTTTTTAAATTAAACATTGATGTATCAAAATTACCGTTTTCATCTCAACCACCATTATAGTAATTTTTAACGATTAGGCTATTAATTGAATCTTGTATCTCTCTCACTTTTTCATTAAGTTTTCTTGTGTTAATTGTCCCATCTGATTTTAAACAAGTTGATAAATCAATTACTGGTGTATTGTTTTTGGTTTTAATTAAAATTTCAATATCATCTTTTTTGTATGGATGTTTTTTCTTGTGAAGTTTATAAACAATTGCTCTACCATCTTTACTAAATTTAATTGGTGTTCCCATACCATCTTTTAAAATACCATCATATTTTGAAAGTCCTGGTTTTTCAATAACACTACCAAATTTAAAATTATCATTTTTTTGTTCTTGACTATTTTGATCATTATAAAAATAATATTCAGCTTTTAAACGACTTGGATCTTTAATGTCAGGGCCAAATGATGGGTTAACAGTTGCAGATTCATGATCAAATGAATTCATAATAAATAAATCATATGGTTTAACATTACGTAATTTTGCCGCCTCAAATAATTCATCGTATTTTTGTAAAAAACGACGTTTTTTATTATAGAAAAAGGCTTTAGGATCATCAGCATTAACGGAACCAAAAATATCTTTTTGTGTTTCTCAAACTGGATTTTTAGGATCGTTATTTAATGAAAAATTAAAGAATGACCCATCTTTTGTTGGATTATAATCAACATCAGTTTTTCTAAAGGTAATTCCGCTAGAACGTGCATTTAAATAATCTTGTAATGCCTTACCATTTACATATGATTCATCACTAACTCCCGATCTAGAATCAATTCCTCCACCAATAACTGAATCATTACTATCACTAATATCTTTCATGTCTTGTAATGTTTGGTGATGTCCATATTCGTGTGTACTTACATATTTTAAATAGTTAATACCTGTTGTTTTAAATGCAGGATCAGATTGAGCAACTAATGATAAATAAGGAATTCGATCAGTTACATAAATACCTTCGTATTCACCATTAACAACTTTATATGTATTTTTACCATATTTATCTTGAACAACTTCTGTATGAATACCATTAATTTTTTGTAATAAATGACGATTTTTATTTAAAACCTTATCTAATAAACCGCTATATGCATCAACATAAATAGTATAAGTTTTAATAATTTTGCCGTTAACAAATTTACTTCCTTGTTTAATCAAACGAGGTGTAAAAACATCAAAATAATTAGCCGCAACTTTAAGAGTGTTGAAAGCATCATCAAAACTTGAATCATAATTATAACCTTTTGGATTATTTTTAGGTGATTCTAAAATTAAATCTTTATCATCTTTTGTTGTTAAAATTAGTTTATTTAAACCTTCGACTTTTGTTTTAGTAATAGTTACCAATGAAACTTTTTTCTCATCATAGTTAACTAATTCAGATTCTAATAATGATTCTTTTGATTTATAAAAATGGGGATTTTTACCATTACCATCAATATCATAAATATAAAATTGTTTATTTTTAAATGTAGATTCAACATCATAAACATTTTTAAAATTTTGTTTTTGATCAATATAATCATTAAAATTTTTAGTAATTATTTCACGTAAACTTGATGCTAAAATACTATTATCATTTTGAATAAATTCATCATTTGAACTTGATTTAGTAGTGAATTCATCCTTAAACATGTAGTATTTAATTTTTTGTTGTTGGCCACCAATGGTTGCATCAATTAGTAAATAATTTTGATTACCACTTGATTTACCAATTGATTCAAGATCAAGCGAACCACCAAAAGGAGATGAAGCACTAGCATTAAATTCCAAAAATTCATTAATTTTAATTTTATTAATTTCATCCATTTTAACGTCGATTTTAGGATTAAATTTTTTATCTTTTGGAATTAACCCTTTAACAAATTGTAATTCGTTTAAATATGCTTGTACTTTTTGTTTTGCTTGTTGTTCGTTTTGAGCATAAAAAAGATAATTTAATGTTCGATCACTTGCTTTAAGATTTTTTAAATTAGGAAAATTTAGTGGATCTTTTGTTAAATCAAAGTACTTATCAGCATTAAATAAATAAGTATTTTTTGCGTTTTTTAGGACATCAATTAATTTAACACCACCAAAACTATGGCTATTATATGCTGAAATCATTAAAGGAATTTTTTGATGATATTCTTCTAATTCTTCTAATGTCATAGCATCTTTATTAACTTGTCGTGCTAATGAATCATAAGCATTGCCGGGGCCAGCATTAATATTATAAATTGGTAATGAACCAAAAAAAGCATCTGGGAAGAATTCAATTACTGTTCTTTCTCTTGTACCACTATGTAACCCTAGTGTAATTGCGTTACCATTTTGTTGAATTCCACGACTTAATTTAAATTCTTTCAAAGTCAACAAATCCGGTCCTCAAGAAACATTTTTAAAGAATCAATTAGTAAACTCAATGAATTCGTCTGGAGAAATACTATTGATATAATTGTTATTGAAAACAATTGGGCCCACTTCTAATCTTAAATTTGGTAAGTTGTTGTTATTTAAATTAACAAAATTATCTAAAAATTCAAGATAAGAAATTTTCTGACCTTTTTTATATTTTCCAAAATCAATTAATAATGTTACTTGTGTATTATTACCTTTTTTTGCTTTAAAATCATAATCAGCAACTGGTTTGCCAAATTGATCCATTAATAAAGCACGATCTACTTGACGATTAGGAATTGTCGTATCAAAACTACTGTTAACTTGTAATGTTTGACGTCCAAGTCCACCAGTAGAAACAGCATATAATTTCATAAAACCAACAACAGCACCAGCAAATGCACCAACTGATGCAAAAGCAATTGCAATTTTAGCTGATAATTTTAACCTTTTTTTCATATTGCTTTCGCTCCTTTTCTAATATTTTCCTGCTTTTCAATTAGCAATTTCTTCATCTGTCCCAAGAATATAATGATCGTTTCCTAAATGAATTCATTTTGGTTGTTTTGTTGGTGTATATTCATGCATGTTAGCATCATATACATAACCTAATAGTGATTTTTTTTCTGAATCAATTGAAGGTACAGCTTCCATTAGAGAACGTGTGTATGGATGTAAGAAATTATTAACAATTTCTTTTGTTGGACCAATTTCTAACAACACACCCTTATTCATAACTGCAATTCGATCTGAAATATATTCAACCATTCGTAGATCATGAGCAATAAATAAAATTGTTAAATCATATTTAGTTTTTAATTCTTTAAAAATATTCACAACTTGAGCTTGAATTGAAACGTCTAAAGCACTAATTGGTTCATCAGCAATTAATAACTTCGGACGAAGTGCTACAGCTCTTGAAATCCCAATTCGTTGTTGTTGACCACCTGAAAATTCTAATGGATAACGGCGAAGAACGGTTTCATCTAAACCAACTGATTTTAAAACATCTAAAATTAAATGGCGCGTGATGTCTGTTCTTGTTTTTGATTTTCAAGTATTAATATCATTAAAAGCTTTTTGTAATTTTTCATCATTATTATCTAATGATAAATGATTTAATTCTAATTCATTGAATAATTGGATTAATTCTTGTTGAGCAGTTTCATTTAAATAATCAAACTTTAAATCTTGTGCTTTTTCTAAAAAAATTTGTACTTGTTCATTATTTAAAAAATTTTTTGCTTTAAGTTCTTTTTTTAATCCTCGTAAATAATCTTTATAAATTAAACGTAAGAAAGATTTTTTCAAATCTTTAATATTATTTAATCCTTCTGAAACAACTGTTTCAATATTTACATAAGGATTAAGTGAATTCGATGGATCTTGAAAAATCATCTGCACATTATTAACCATAAAATCAGTTAGTTCTTTAGTAACTTTCTCACCTTGTTTAGGCAATTTAATACCATCAATAATAATATCCCCAAAACTATGACGCGCTAAACCAATTAACGCAGATCCGGTTGTTGATTTACCAGATCCTGATTCACCAACTAATCCTAAAACTTCACCTTGATAAATATTAAATGAAATATCTTTAACCGCTCTAAATTTTTTAAATCCATGACCATAAGTAACATCTAAGTGACGTACTTGTACTAAACGCTTTTTTTCAGGATTTGCATTTAATCGTTCTATTAAACCTTCAGTAACTGGGCGAATAGTAATTTTGTCTGGTAAACAAAAACGTTTTTTGTGATTAAAAAAGGCTTTTTTCTGGTTTGTATCACTCATAATTGATGCTCCTTATTTTATTAATTATTTTCTTGTCGTTTTTTAAAATTATTTCATTTATCTAAAATTATTTGTGGAGGTGTATACTTTTCAGCACGTTCATCTAATAATGCTGATTTAACACGATGAGTTTCACTTACATGATACATTTCAGGTTCATGTTCAAAATCACGAGTTAATGCATAATCATTACGAATAGCAAATGCATCACCAACTATGTCATTTAAACGTGATGGAACAGAACCTTTAATTGTTGCTAAACGATCACCTTTATTAACGTCAGGCATTGACATAATTAAACCTCAAGTATATGGATGTTGTGGGTATCATAAAATTTCATCACGCTGACCTTCTTCAATAATTTGTCCAGCATACATAATAGAAATATAATTAGCAATTGAAGCAACCACTCCAAGATCGTGAGTAATAAAAATAATACACATTTTAAATTTCTCTTGTAACTCACGAATAACATCTAACACTAAAGCTTGCACTGTTGGATCAAGCGCTGTTGTTGGTTCGTCTAAAACTAAAATTTTTGGATGTAATGAAACAATTGAGGCAATTACAACTCTTTGAATCATTCCACCAGATAATTCGTGTGGGTATAATTCCATAATTTCTTCAGGATTATTAATTTTTGTTAATTTTAAATATTCAATTGATTTATTATAGGCTTCTTTTCTTGTCTTAACAATTCCATTAATTAACATCCCTTCCATAATTTGCTTACCAATCTTCATGGTTGGATTTAATGTTGACATTGGATTTTGAAAAACTGCAGAAATTACTTTCCCTAAATAACGTGATTTCTCTCAATCTTTGAAGCTAAAATCTTGAACTTCATTGTTATATAATAAAATTTTACCTTCTTCAACATTGGCATTTTTTCCAGCCAAACCATACAAAAGAGAAGTAATTACTGATTTACCAGATCCTGATTCACCAATAATTGCATGGACTTCACCTTCATAAAATTGAACAGATGTATTTCGTAAAACTAAAGCACGATCATTGGGTTTTGATGGGTTTGTAAAAGTTAAACGTACATTTTCGATTTCAGCTGCAATTTTTTTAACTGTACCATCTTTAAAACTTTTATATTTAACATTTTTTTCAAATTTTTCAAAATCAAAGTTTTTTCGTGAATTAGCAGCTTTAAAAGCCATTGTTTGTTTTTTATGTTTTTTATTAGCTATTGTAGTTTCTTTTTGTTCACTAATTTCATTAATACCACTAGCCTGTTCATTAATTAATGTTGTTGCTAAGATTTCTTCATTTAAATTTTGATTTTGATTATTATCTTTAACATTAATTTCTTCTTCTAAAGTAACAATATCTTGAATATTAACTTTTTCATTAGTTAATTGTATATTAATGTCTTCTAAATTATTTAATTGTGGTTCTAAATCCACCTCTTGTTCATTTAAACAAACATTATTTGAAGTTAATTTTAAAATTTGTGGTTTTAATGTTTGAATAAAAGCGCGTTGACTACGAAGATAGTTAATATAAGTATATCGGTTGTTTTTTTGTTTCATAAATTATCTTTGACGTCTTAATGCGTCTTGTGTGGCACCCCCAACTAATTGAATTGAAGCAACTAAGCAAACTAGTAAAAATGATGGAATTAAAGTAAAACGTGGATATAAAGTAATGATTGATGAACCATCTGCAATTAAATTACCTAAAGTTGGTGTATTTGGTAAAGATAAACCAATAAAAGCAAGTGATGATTCAGCTAAAATAGCTCCTGGAATACTAAATACTAATTGAGTAATCATCATTGGAATTAAAATTGGTAAGAAATTTAAAATAATTTTTCATGCTTTTGTTCCTAATGTTTTTGAAGCTGCAACCCATAAAAAACTACGAGCACGTTTAACTTGTGAACGCATTTGATTAGCCATTGCAATTCAACCAGAAAATGAAATAGCAAAAATCATAACTCCAAATGATGGTTTTAAAATAATTGTTAAAATCATTAAAATGATGATTGTTGGAACGTTTGTTAAAATTTTAATAATGAATGTCATGATTTTATCAAACATTTCAAAATAACCCATTAAAATCCCTAAAGTTACTCCAACTACTAATTCAATAGCAGTTACAATAATAGCAATTCCTAATGAATAACGTAATCCTCACCATAGCATGGCTCATAAATCACGCCCATTATTGTCAGTTCCAAAAATAAAACCATCTGTAAAATAATCTAAATATCGTTTATTTGGTCGTAATTCTGTTGGTGATCCTGTTGTAAAAGGAATAATAATTGCCAAAATAAATAAAATAATAATAATTGCTAATCCGATTAATCCAGAAAAAGAACGTCCGTATCGGTTAGCAAATTCACGAAAAGGACGAGGGCGCCCTCCCATGTATGCATTATCTCTAAACTCAATTAAATGACCGATAATCTTTCATTGTTGGTGTTGAAAAGGTTGTAAAAACTTATTTGGTGCATCAGATGTTAATTCATCTATAAATGATGAAGTAATACTTTTTGATTTTTGTTTTAAATATTCTCCAAACTTCGCCATAAATTTATTTTCCTCCTCTTCTTATACGTGGATCAATAATTTGATATAAAGCATCTCGAATTGTATAACCAATAATTGTAATTAATGAATAAATAATTACTAATAATAAAATCACGTTGTAATCTTTTGAGATAATTGCTTCAAAAAATAATGAACCTGAACCTGGAATTTGAAAAATTTGTTCAATAAACAACGCTCCAACAAATGATCCTAAAATTACAAATGGAAAGAATGTTGCAATTGGGAATAATGATGGTTTTAAAGCATGTAATCACACAAAACGGTTAACAGTTGCACCTTTTAAATACGCAAATTTCGCATGCATTGAGTTTAATTCACGATTCATTTCAACTTTAATATATTGCACATAAACAATAATTGAACCAAGTGATAATGCTAACCCTGGTAAAATATAAGTCACAAAATTATTAATATCAAACGTAAATGGTAATCCAACTTCTTTACCAATTAATAACAAGATTAACCCAAATACAAGCGAAGGTACAGCTGAAATTACAGAAATAATAACGGTTGATACACTATCTAATATACCTCCTGGTTTACGCCCAACAAAAATACCTAAAGGGATTCCAATAACTAGAGTTAATAATACTGAAAAAATTCCAACAGCAAAACTTACTAAATAACGAGATCAAATAAATGAATTAATTTCAACCCCTGGGCGAATTGAAGTACTTACTCCAAATTCTCCTCTAAAAATATTTGCTAAATAATTAACATAACGAACGCCTATTGGACGATCTAAACCATACTGTGCTAGAATGGCTTTTTTCGCTTCTGGTGATGTAATCCCGATCGTTAAAGCAGATTCTCCAGGTACTGAGTTAATTAAAAAGAATGTAATTGTAATTACAATTCAAGCTAATAAGAAAAATTCAGCAATTAATTTCAAAGATTTAATAATAAATGTCATAAACTTTGAATCAAAAGTGAAAAAATCGATTAATGGATCAACAACACGATCGCGATCTGCTGCTGTTTTTAGTGAACGACCACCACCAAAAATAATAAAATTATTACTAAATGAATAATTAACTCGTTTTTTTTGGTTTAATATTGGCAATTCACCATAATTGTTTTGCGATGAATTAGCTTCAAAGCTGTTTTTCTGATTCATATAAATCTCCTATTTTAAGTAATTGATTGTGTGGTTTTAATAATATAAGCAATCATACAAGCTAATGCATAAATAAAACTAGGTAAAATGATAATTGTAATTAAAATATTTAAGCGAATTGTGGCATAATCAATAGATCGTAATTCATATTTAGCATTGATATAAAAACGATCAACTAATTTCATTTCTCACGAACTAAGTTTATACTCATTATATTTTCTTAATTTAACTGCTCAACGGCGACGATAAACTTGGCGCGAGAAGATAATATATAAGAAAACTACCATAAAGATACATGTATAAGCTATAAAAACATTCATACGGTATTGGTGATAATCTTTAATTAATTCAACAATTAAGTGACTAATAACCATCAATATTAATAATGCAATTAAAACAATAATGCTTTTATAATTTAATAAACGATTCGGAAACGATTGTTTTTTTATACTCATTATTATTTAATAATTACTCCTTTTCTTAATAACTTTAATGACAAAAACCATCAGCATGGCAATGATGAATACTACCTGGTTTTTTAGGTTCAAGTGTTAAATCAAAATAACTATACATACTATAAATAACATACATTCCTTTAATTAGAGCATTATTGTTTTTTTGCCCCTGAATTAGAAGATTTTCATATAAATTATCTAAATACTCTTTATTTGTTTTTAAATTAAAAGGTGTAATTTTTTTACCATTCTTTTTAATGAATGTTGGTGCTAAAAATGCAATTGGTGAAAAGTAAAAATCATCTAATTTATTATTTCAAATATTGGTATAAACAAGATCACGTTCGGGTTTAATAGATGTTTGTGCAGGACGATCAACAATAAAAATTTGTTTTAAAACAAGATCATTTAAATTAAATAATTTAACATCGTTATCAATATTTAAATCATTAATTTTACTAATCATATAACGGACTTTGTTTTGATTATCATCGGTTGGTATAGCTTGTTTAACTCAACGATATAAACTACCACCAGTTATTGTATATCCTTCATAATGTTCTTTGCTATCTGGATATTTAATAATCGAATGATAAATTTTGACATTTGAATAATTAATTTGCATGCTAATAGCTTCATTAAAATAATCAATTATTGCTTTTTTTGAACCAAATCAAAAACGTTTAATTTTCTTAGTAAAGTGATAATCATCATTTCTCACTTTTTTGTCAGGTGGAATTGGCTCATAAAATTGAGGTTTAGTTTGTATATTAATATCACCAATGTCATCAATAACTAAGCCTAATTTATAATTAATATCAAAAGTACCATTATTTGAATTTTTATCTGTATTGTAAATTAAACTAAAATCATTTAACTTAACATGATATTTTGCATTATATAAATTTAGTAAAGCAAAATTATAAGAATAATATAAATACAAGGAGCGTTTAATTGTTTGATCAAAATAATCTTTTAAACTCTCATAGAGGTTTAGAAAAATTTTAGTTTCTTTATTTTGAGATTGATTTAATATTTGTTTTTCATTTGCAAGATTAATATTATTATTTGTGATTGATTGAAACTTGATTTGCGTATCTTGATTAATTGTTACTTTTTTAGATTTTTTTGTTGAACAACTAGTTAATAAAACTAAACTAATAATAGTTGTTAATAATATACTAATAAAGATTATTAATCTTGCTTTGAGATTACTTTTTGTTTTCATATATACTTGCCCTAAAATCTTATGTTAAAGAGCCTACACTTCCTTTAATTTATTTTGATTTATTAAAAATACATTTTGTTAATAATGCTTCAACGCTTATTAATTTTAATTGTATTAATATACATCTTATAAATAATAGAAATGCAAATAATAATAAAACGAGAGCAAAAAATACGTTTGCCATAAAAAATGCGAATGGTATGATTAAAAAATTTATTTTGATTATTATTTAAATTAATCATCGTATTTTTTTCTCCTTTTGGTTATTGTGTTATATTATAAGGTTTTTTTACTAAAATATATAATTAAATACCAAATATTACATTTTTTTTTACAAAAATAATCATTAATAAACTTATTTTTAACTAATTTATTTCAAAAAAAATAAAAAAAATGGTCACGACGACCATTTTATTTCTCATTAAATTTTTAACTAATAGTAGATTTCTTAACAACCATTTCTCCATCAATATCAATAATTGGATAGATCACTATTAAACAATTCTTATCAATATCGCGCACTAAATGAATTAAAGATGGTAACTCAATATATGGAGAAATAATTTCTAATGAATAATTTGTTCTTAATGTTAATGAATCACTTAAACTATTTAAAGTAATTGAATTATTAAAATCATTAGCACGTAAATTTTCAATGATAGCATTAACGTTTTTTGAATAAACTTGAATTTTCAAACTTTTATGTTTTGGAAAATAAATGTTAAAAACAATTCCTAAAACAATTGAAGCAAGAATTGATGCTACCATATTTGGTGAAAAGAAATATTGTGCATCTCAATAACGAGAGTTAATCATACCAGCAGGAATGAATGATCCTAAAACAATTCCAATAATCAATGTTGCAACATTAAAATAGGTTAATATTGAACCGGTAGGTTTTCCATATTTTTTAGAATATCAAAATGAGATAATATCTGTTCCACCTGACGAACCTCCAGCAATATAAACAATCGATAAGAAGATACCATCAAAAATTGGATAAATAACTGCATAAATTAGAATTGAAGGAATTTTACTTGCTTGATCAGGATCTACTCAAGATAAAATTTGTACTTTATTAATAATAAGATCATGAAAAATATATAAGGGCTTAGCAAATTCATACAAAGATTGTCGATAAGTCAAACTTTGGTTTAAATGAAATAAATCACCAATTGTCGCATTAGGATTTGCTTGTACATAATGCATAAATTCATCAGCAATTTTTGAATTAGTAATATTAGAATAATTCAAGACACCAGTTTCAATATTTTTAACTATTTTTTCATCTAAAATACCATTAGTAAAAAACAAATTAATAGGTGGATAACTTAAACGGTTGTCGCCAAAAATCATAATTTTTTCTACGTTTGGAATTTGACTTAAAGCAAAACCAAAAACTTGCACAGTAATAATATATGTCAATGTCATTAATGTAAAATGCTTGCCAATTTTTTTATAAGAAAAAATTAATAAAGGAATATTTACAAAAATTGCAAATAATCAAAACATAATATCGTATGCTAATTTGGCTTCTTTTGGTGAACTATGATTTGAAATCATAATTGATTGTAAAATTCGTGCGATCCCTTGTGATGTTCCCATAATGCCAGCTGAATAAATTCCAGTATTATGAACTAATAATAAAACTAATAAACTCGCAAGAAGTGAAAAAATAACAATAATTAAGTAACGTAAGAAGATTTTTTTAGTTGAATAAAGAGCGGCAAACTTTAAAAGGCCTGCTTTATAACGAATTTTTTCAATTTGTACACTTTGTTGAATAGTTGCTTCGTTGCTTATTTGTTTTAATAAATTATTTTCTGTTCTTAATACTTCTTTAGTTTTATTTGTTCAATTAAAAATCTTTTTAATTTTGTTTTTATGATGCGTTTTTTCTTCGTTATTACTCAATTAAATCAACCCCTTTTAGTCTTTTTATAAAAAGAAAGAATTTTATATTCTACTCAAAATAATATTTTAAGGATAAACATAAAAAAAACAACAACTATTTATAGTAATTGTTGTTTTTATTCAAATAAAACATTTTATTGTTTATTTAATTCTTTAATTCTTGCAGATTTGCCTGAACGATTACGCATGTATGAAATATAAGCACGTCTTACTTTACCATACTTGATAACTTCAATTTTTTCAACAAGTGGAGAATGAATATTGAATGATTGTTCAACACCTATACCACTTGATTCTTTACGAACAATAACTGTTTCACTTGAACCAGAACCACGACGACGTAAAACAACGCCTTCAAACTTTTGAATTCGTGATTTTTTTCCTTCTACAATTCGATTATGAACGATAATTGTATCACCAGAATCAAAATTAGGAATATCTGTTTTTAATTGTGTTGAATTAACAAAATTCATAATTTCGCCTTTGTTAATCTTAAATAAAGCCATTTTTTACACCTACTTTTTGTTATTTAAATATTTTTGATATAAATCAGGACGATTCTTTTTAGTAATCGTCTCTTGTTGCTGTTTACGAAAATCAGCAATATTTTTATGATGACCAGATAATAGTATATCCGGTACTTTATGCCCTTCAAAAACAAGAGGGCGTGTGTAAACAGGATAATCTAATAAGTAGTTATTAAATGATTCAGTTTCTAAACTAGAAGGAGAAATAACGCCATCTAACAAACGAATAACAGCATCAGAAATTACCATACTTGGTAATTCCCCCCCTGTTAAGACAAAATCACCGATACTATATTGGGCGTCAATGTAATAATTAATACGTTCATCAAAACCTTCATAATGACCAGCAATAATGATGATATGATCATATTTACTAACAAAATTGTTTGCTAATTCTTGATTAAAAACTTGACCTTTTGGTGTTGTTAAAATCACATATGAATTAGAAGAACGAATTGCACGAATGGCAGCAACAATTGGTTCAATCCTTAAAACCATACCTGCTCCGCCACCATATTGGTAATCATCAACTTTTTTATGTTTATCATTTGTATATAAACGAAAATCGATAACTTCTATTATAACTTGGTTATTTTTAATAGCATTACTAATAATTGAATGCTTTATCCATGTTTCATATAATTCAGGAAATAAAGATAAAATCGATATTTTCATTAATGTTATAAATTATTTAGCTTTACTTAAAGCAGCTTTACGTTTTTTATTTGCATCTTTTAAAGCAATAAATTCTTTTCAAATACCTTCTTGTGATAAGATATTTTTTACCGTTTCACTAGGTTGTGCTCCGTTTTGTAATTGAGCTAAAATAGCTTCTTTTTTTAAAACAACTTGTCCTAAAACAGGATCATAATGACCTAAATTTTCAATATATGCACCATTAGCTTTGGTTTTAGCATCCATAACTACAATACGAAAAAATGGTTTTTTATGTGTTCCTACTCTTGTTAATCTAATCTTTACCAAAATTTTCACCTCGTTTGTTTTATTATTAAAATAACTTTTAAATTATAAATAAAAAAAACCAAGCTGTCAAGTATTTTTACTTAACGCTTTGGTTTTTATTTTAGTAAGTAATTATTAATAAATAATGCAACAGAATTAGAATTATTTTTATAATTAATAATCACATCAACATGTTCAATAATTGTTTTAGATTTAGTTCTAGCAGCCGCTGCTAAACCACTAATTTTAAACATTGGAATATCATTAGGCGAATCGCCAATCGTTGCAACCTTATCTAAATTAATCTGGTATAAATTAGCAATAAATCGTAATGATGATGCCTTATTAATATTTTTATTAGTCACTTCAACAATGTATTTTGAGGTGAATGAAATATTTACTTGATCACTAAATTCTTTAATCAATTGATCTATAACCTCGTGTTTTAATTTTTTAACAAAATTTGAAGGTAAAATATTAATTTTATAAAATTTATCATTTTTATTAAAAATTGGGTTTAAACAAACTTTTCTTTTTGTGTGATGAAGTTGTAATAAAAATTTATAGTTAATATTATAAACATCTAAAAAATGTGTTTGCATAAACCTTTGGTTATATGGTCAAAATCCTAATCCATGTTTTAATGAAAATTTATAGATTTCATTCACTATATCTTTATTAATTGTTGATTCAAACAATACTTTTTCATTAATAACATCATAAATATATGAGCCATTAAAACATGAAATATATTGGATTAATTCCTTGATTTGATCATGAATTAAATGCACAATCTTTAAAGTATTTTCTAATGAACGTCCTGTACTTAGTACAACCTTACCGCCTAAACTAATATATTTTTTTAATCCTTGTAAATTAGCTTTACTAATGTTTTTTGTTTTCGTTAATAAAGTACCATCTAGATCAATAACTAACATCTTATATTGCATATTTTATAATTTCCTTAAAGGCTCTAATTTTTATAAACCGTTTGAAATTAAATCATCAATATCAACTTCTTGTTCAATTTCTTCAACTTCAGCTTGTTTGATAAATTCAACATAATTATTGTCTTTTCTAAACTGTTCGACCAAATCATTATTAATAATTGTGACATTAATAATTGGATTTTTCTTACGTCATTTTCAAACAATTGATTTAACATTTCCACTAACTATTTCTTTTAGTTTTTTTTCATCAAATTCTTTTTTTGCCAACGCTTCTTCTAAACTTGATTTTGCTACATTAGAAATTTTATTAATTAAATTAGTTGATTCTTTGGCAAAAAAACATCCTCGTGTTGAAATACTTACAGCAGAGTTTAACTTAGCTTTTTTAAAATCAGCATTTAAAACTAGATTTAAAATACCATCACTGGATAAAACAACACGTTCACTTAATAATTTACTAATTTTAGAACTAATTTCATAACCATTAATAAATTTAGGTTCAGCATCAACATAAATATTAGTATGACTTAAAACACCATCTAATAATTGTAATTTTTGGCCATTTGGTGCAATAATGACATGGTCTTTATCAAAACCTTGGTCAACAGCATTTTGTTTTAAAGCACGAAACATTTTATATTCCCCATGGATTGGAAATAAATATGATGGATTAATTGCTTTAATCATTAATTGGTGTTCACTCCGTGTGGCATGACCAGAAGCATGAATCTTTTTGTTAGGTGAATTTTCATAAACTGTTAATCCGAGTTTATAAAGTTTATTAACCATTGCTTCGACTTGAGCATAGTTTCCTGGGATTGGATTTGATGATAAGATAATTGTATCTGATGGTTTTAAAGTAATTTTTGAGTGGTTATTATTAGCCATAACATTTAAAGCCGCCATTTTTTCACCTTGTGAACCAGTTAAAATTACAACAATTTCATGATCTTTATAAAATGGCAATTCTTTATGTGTGATAAATGAAGATTCAGGAACATTTAAATAACCCAATTTTCGACTTGTTTTAATATTAGCTTCCATTGATTTACCAATGATACAGATTTTTTTATTTAAACCAACAGCTATAGCAATAATTTCTTCAACACGACCTAAATTAGAAGCAAATGTTGAAATAAAAGTTCGTCCTTTAATATTTACCATATAATCACGAATGTTATCAATAACATATCGTTCACTTTCACTAAATCCAGGAATTTCAGCACTTGTTGATTCGCACATTAATACTGAGATTCCACGATTAGAAATTTGTGAAATCTTAGCTAAATTAGTCTCATCACCAGCTGTAGCAAAATCAAATCTAAAATCACCAGTTGTTACAATGTATCCGTTTGGTGTTTTTACACAAATTCCAAATGAATCAGGAATTGAATGACAAACACGATAAAAATCAACTTCAAAATATTTTGTTTTATACATTGACTCATCTTCAAAGACAATAATACGCGGAGGTTTAATATCTTTATATTCACTTAAACGACGTTCAATTAATTTTGCTGCTAATGTTGGTGCATAGATAGTCTTAATTTTAACATGTCGCAAAATGTGAGGAATCCCCCCAATATGGTCTTCATGACCATGAGTAACTACTAATGAATGAATTTTATGATTGTTTTTTATTAAATAATCAAAATTGGCAACAGTTCCATCAAATCCTGGTAAAGATGCATTAGCAAATTTAATGCCCGCATCAATTAAAATAATTTCATCTTCATGTTCAACAACATAAGTGTTTTTACCAATTTCTTCTAGCCCCCCTAAAGCATAAACATATGTAGGACTTTTTTTGGTATTTTCCATTTTAACTCCTTATAATTGGTTTTATTATTAAAAATTTATTTATATAAACATATAAACAATGTTGTAACATCATTTATACAAATCTTGATATATATTATTAATAATAATTATATTAGAAAAAGTTAAAAATATGAAAAATGATTATTATTTTAGTTGATATTGAAGTTATATTTAATAAATTAAAAAGTATTAAGAATTATACTACTCTTAATACTTTGTACAATTAGTGAATATTTTGATTAAAAATTATTTTGTTGCCATAAAACATAATCAATTTTATTATTGTTATTATTTGATGACAACGAATCATTAATTCGTGACACAATTTTATGTTTATCAATATATCATTCATACTCTTTAATTCTGTAAGTTGAATAACTTCGATCTTCTAAAAAATATTGTCGATCAATATCCTCAAACATAATCTGAACACTACGGTTTTCTTTTCAAGTTTCTAGTAGAATTGTTTTTACAATTTCTTTAGTTTCTTTTTTAATAATTACTAAATCAATTTTTTTAGTACCAATGTTTCAATTTGGAAAGATTTGGTATTTATTTGATAATTTTTTTGTTAGTTCGCTATAGATTTCTTTAACTAAATCATTATCAAATTCCAAATATGTTTGTTGTTCTAAACTTTCAAGTGAATCATTAATACTTAATTGACCATTAATTCGATCAACATATTCAATAAAGCGTTTAAATGTCAGTGCATTTTGATTATTAAGATTTGAAACTTGAATTTGATGACCATAAAGTGATTTTATAACAATCATTTTTTTACGTGCACGAGTGATCGCTACATTTAAACGATTCATTCCGCCCTTAGCATTAAGTGGTCCAAAATTATTACGTAAATTTCCTTCAGGATTTGGGCCATAAGCAATTGATAAAATAACTAAATCACCTTCGTTACCCTGAACATTTTCAAGATTAGTTATCACAATTTCATTATTTTCAATTTTGTCACACAATCCCTTTTCAAATATATTCATTCGTTCAAAAAGTAGGTTTTCAACTAGACTAGCTTGTATAGCATTAAATGTTACAATTAAGATTTTTTCATATTTTTTTCAATTATCAATTAATGTTGCAATTGTTTGTTCTGCTTCTAATGGATTACCTTTTTCTCAAACTCCATTAACATTAATAACGTCAATTCCTTTTTCAAAAGCACCTTGGCGTGTGGCTACTTCTAGATTATTGTTATAAATGTATTTATTTGAAAACTCGATTAACTCTTTTGAATCAGAACGATAATGATTTTTTAAATGATATTCATTTCATCATGAAGTTTTAGCACGTTCTAATAAAGAATCTACTTTATCAAAATCATCAATTTCAAAGTCACTATCAGCTAACTTATTAATAAAGAATGATGTTGGCTTTAATTGTTTATCATCACCAGAGACAACTTTAGTTTTACAACGATACACAATAGGATATGATCGTTCAATTGTCATTTGGGATGCTTCATCAAAAATTCCATAATCAAATTCATTCTCAACTAATGGAATTAAAGATGCAACATTATCTGGACGCGCAACCCAAATCGGAAATAAACGTTTTAAAGCAGTGTAATATTTAGATAAATAACGTGAAATTGATGGTCAAGAACGTGATGAAGCAATTCGTAATGCGTTAGTAATTTCATCTTTTTCTTGTTGAGGTAGTTTTGTTAAATAATTTCTTAAATTATTAATATATTTTTTAAAAACAATTTCACCAACAATTTTAGCGGAGCGCATTGATTCACGTCTTAATTGAATAATAATATCTTGCAATAAACGCCCTTTAGTTTCAGAAAACATTGGAATTATATGTGTAATTTCATCTCATAAAACAATCACTTTTAATTTCTTAAATTGATCGTGCGTGATTAATGTTGAAACATGTTTCATAATTTTACCAATCTTTTCAAGATCAAATAAAATTAATGGTTCTAAATACAGCGTTTTAATATATGGTAAAGATAATCATGATCATTCACGAATAATATCTTGCATTGTTGGTGTATAAATTTCAGCAAGCGTATGTAATTTTGACAAAAAATCAACATCCTGGTTGATAAATTCACGATACGAAGCAATTTGGTCCATTTTTGGTTTAGATAAATATTCAATATTATTTAAAAAATCTATGAATAATTCAATTACTTCTTGACTATCACGATTGCGTGTTTTAATTTCTTTTAAACTTAATGATGATTCATCGCTTGGGTCAGCGTTAGTAATAATTGTACCGTTACTAATAAATTCAGCAAAAATAATTTGTTTGTTTTCATCACTTGCATTATTATAAATTGATAAAAATGCTGAAATACGATCTTTGTTTTTAATTAGAAAACTAAATCATTCATTTTTGGCTAATAATTTTTTGTGTTTTGTAAAAAAATTATCACATAAATCAATAATTTCATTTAGTTCTTGTGTTGTTTTTGATTGCAAACTAAAATCTTTATCTATCACATCTTTACGGAACTTTAAATAATCACCAATTTTGTCTATAAAAATACGTTTATCACGAATTGATTTATTCATAAAAATAGTGTTCGATTCAAGATTATGTTCAGCTAAAAAGTTGTAATAATTATCAATGTCTTTGGTGATTCTATTAGCATGCATCAAAAATTTTGTCACTAATTCATCATTGGTTTGAATTTTATTGGTAATCATTTTTAAATGTTTTAAAATTTCTATAGAACTATAATTATCTGTTAGCTTATATTTTTTAATAAAAGAAGTAAATCTTAGATAAGCTGTAAATAAATCTTCAATTTTTAACAAATTGTATTCATTAAAAATAGTGCTGATCTCTTCAAATAATGTTGTTCGTTTTTCATTTTCTTCATTAAATTGTTGGGGAGTTAATCATTCTTTAACAATTTGTTCTCCTAATTCATTTTTGGTAGCCACATAATCAGCATAGTCCATTTTATAAATGCCATCATTATAATCTTCTATACTCCAGTGTTTTTTAACTAATTGTAGTAATTCAGAATTTCAATCCTCATAATTTTTAAAAAACATTGATTCCTCTGGAGTAAATTTAATTGGTTCTATTTCTTTAATCTTTGTAGAACGAGATTGTTCACGATACCATTGTGTACCTAATAAATCATTTAAATTAGAAATTTTTTCAAAAAAAGTTTCTTTGTTTGTTAAATCATAAACATATAAAGCAAATTGTGAAAGCGAACCTATTCTTTCTGTTAATACATCTAAAGCCGCACGTTTTTCTGATACTAATAAACTACTTTTTCCTTTTAATAAAACATTAAAGATAATATTTGCAATTACTTCAGACTTTCCTGTTCCAGGAGGTCCATAAATCAAAGTATTTTGTGAAAGTGATGATGCAACAGCATATTTTTGAAAAATATTTAGAGGACGTCCAATTTCCACCACCATTTGTTCTTTAATTACTTTATCTTCATAATATTCAACTGGTTTATTCAAACCATCGTTTTCTGATTCAAATGGATCAACATCCATTTCAATAATTTTATGTAAATCAGCTTTTAAAGCGCCCCCATCGGGTTCGAATAACCCCAGTACAGCTGATGGTTCAATTTCAAAAGTTGTATATCGATCTAAAATTTGTGTCTCATTTTCGTTCATGAAAGGAACAAATTCGTTTTCGTTAATATTAATTTTTTGACCTGTCATATCTTCAATCATTTTAATAGTTGAAGACAATTCATAATTAGTCAACAATTCTGTTGTTTCAACCTTAAAATCTTGATAACCTTTTTTTAAAAAGATTTGGATTTTTTCATTAACAATTGGCTCATCTTGAATTTTAGCGATAAAAATTTTATCAGCTTCTTCTCGAATTTCAACTTTAAATAAAGTTAATGGTGATTTAAAAGGATCGTTTGGTAATAATTTTCCTTTCAAAAAATTAAAAGTAAAGTAAAGAGGTCAAACAGTTGTATCTTCAAAATAATTTTTTGCTAGTCTATTAATAGAAATAAACTTTTTTCTAAATGCTAACAATTGTTCAATTGCTAAAGGAATAATTTTTTTCTTATTTGCATGAAAATCACGTTGTAGTAAATTAATTTTATTATCTGCTAAATTTACATTGTAATTTACTAAGATTTCTAAAAATTCACCAATATTTAAAGCATTTTTTAAACTTTCTTCAAATAAATTTAAATTCTTACCTACATTTAATTCAACATAACTAATTTCGCCATTAACAAAACTAATTAGATCTTCTTTTTTAATATACTTAAATAAGTCTATATTTCTTGATGATGAAAGTCTTGTTAAAATAATTGAATCATGCGGATTTAAATTAACAAGATTCATCAATTTTTGTTTAATCGCTTTTTTCATACTTGTGGATACCTCGTTTTTTATAATATGATTATTTTAACTAAAAATAAGAAAGTTTTAATATCAAATTATTTATCTTATTTGTGCTAATATAATTCATATTTTTAATCTACATTTAAAAAATAACTTTGTTATAAAGTTGTATAATTATATAACATACAAATTAAATGGATTAAGTAAGCAAACTAAATTTATATGAGAAATAATTTATCTGATTTGGAAATCGAAAAATTGACCAAAACATTAGTATCAAAAAACAAAAGAGTTGTAGCTTTATATGGCGTTCCCGAAAACACTTCTTATAAACTAATGAACGATCTAATTAGTTTAATGGATGTAGAAATTCCTAATTGATCATCAATTTCAAGCGCAACTGAAGACCAATGTATTAATGATATTAAAAATTTTTTACTGTCAAATGCAAGTTTAGAAAACTTTAAAAACTTGCCTTCTAATAACCCTTTAATTAATTGGTGGAAAAGACGCCGTTTAAATACTGTTGTTAAAAAGATTGAAGAAATCAACTCAGCGTTCGCAGACCGTGAAATAACATCTCGTTCAAAAATCTTTTTGATGCCTGTTTTAACAGCATTTAGTACTTTAGGAACTACACTAGCTGTTCCTTTAATATCTGTTACTTTTTTACGGGGTGATGCAATTATTAATTTGTGGGGTAAAGATGGATATATTGCTTTCTTAAGTTTACTATTTTCTTTAGTTGTTGCTGTTATTATTTCAGCTTTTGTCGCTTTATTTAGTAGTTTAAAAAATAATAAACGTAATTATATTGTTTCAAACATGACCAAAGGTCTTAAAAGAATATATGATAAATATTTTATCGAAAGTAATTCTCAAGAAAAGATTAATGCTAAAGTAACTTTTTATTCACGCTTTTTAGAACGTTCTAAATTCGTTGTTCAAAATAATTACACATTTTTTTATGATGTTGTAGATATTAATAGTGATCAATATCCTAAAATATTAAAATATTTCAAAACCTTAAACCAATTAAATAATACAGTAATCTTCGATGCTAGTGGTTTTAAATATTTAGACGAACGTAAAATTTTCCGTAACATTATCGATTTAGAGAAAACAAATGTTGTCCGTTTGGATCGTTATAAAACAAAAACAAGCGGACGTCGCTTAATGAGTTTTATTTTCTATCAATTATCAATCATTGCTAATGTTAATACACGAAAACTTTTACAAAAATTTCCTTTCTTTGTCAACTCTTTATATCGTTTTTTAGATTACAGCGAAAAAAATACTGAGTTATTAACACTTTTATTAGACATTAAAAAACACGCTTCAAAAACAGAAATTCCATTAAACGATGAATCACAATTATTTTTTGTAGACTTTTTTACATTCGTGGTTTTTAAAGCTTTGGATGAATTAGGATTTGAGACGCTAATGAATGATTTAACAGTCTATGGTCGTCCATCTGAAATCACTAAGAAAAATATAACTTATAATTCATTAAAATTGGATTACATCATTAATCGTAATGCGCGTAATTTTGGACAACAAGCATTATTGTTTAACTTATTAGATTATTTTGATGAGACAGTAAATAAAAATATCTTTAACGAATTAGGAAATAGTAATAAAAACATGATTTTTTCTAAAAATCATCAATTAAATTTAGCCAATGAAGCTTTAAGTAAAAAAGGTTTTACAAAGCGTGAAATTGATTTAAATAATCATTGATATGATGCTTTATATTCTAATCTTCATGATGATGAAGACATGTTTGTTAAAATTATTGAAATTAAAGATAATGTTGATATCTTAAATGTATTAGATGAACTCTTTGTTCGTGCTCAAAATGATGGTGTTAAAAATTTATTAATTTATGTTTTTAATGTAAAAATGTTATATTCATTAATAGATAATGAATACGAGTTGGTTAATGAGTCAATTATTTAATACTTAATATATAAGTTTTTAAATTTAATTATTTTATTTAAAATAATAGGAGTAAAATAAAACGATGACAAATGATGAGCGTAAAGAATTTATTAGAAAAAATAAAATTATTTATTTTAATCGAATTCGTTACTTTTTTTATGAATGAACAGATATGAAAGCATATCAACGCATGTTAGTGCCTGAAACTTTTTTCTCATTTTTTTATCAATTATTCGTCTTAATGATTGCTTTTTGTTCCGTTACAATTATTATTTATAATCAAATTGAATTAAAACAAGCTAATGAAGAGCACATAGACTTTATTGCTAAATATTTTGAAGTTTATTATGCTTTTAATATTTTTTTTATTATTGATTATTTAATTCGTATTTCATACGCTGATTACACATACAATTCAATTTCAAGACTAAAGGCTTTTTTAATAGCACCATTTCGTTTTGTTAATTTATTGGATCTAGTATCTTTTGTAGCAATTGTTATCATTTATAATACAGGATTATTAGATTTTGTAGCAAAAATGAATGAAACAACTTCATATTCACAAAAATTTGATTATTATTATCAAATCATCAATAATAATGCTTATATGAATGTTGTTCCTTGTATGTTTTTAATTAAAATCTTAGGTGCGCATTCTAAAATTATCAAAAATGTTATTTTGAGTAGTGAAGTTAATTTCTTTTGACAAATTTTTAAACGGCGTTGACGTGTATTATTATCATCATTCTTAATTTTAGTTTTATTTACTTTTCTATTAAGTTTTATTATTTATAGAATTGAACAAAATTATTATGAAATAAAGCATATTTTGCCTGGTAATCCGTTGTACAAACTTAAAAATTTTGGTGAAACTTTGTGGTTTTCTATGGGAACAATTACCACGATAGCTTATGGTGATGTTACACCTGTTTCTCCAGCTGGTAAAGTATTTGCCGTTATTTTAGGTGTAATTGGTGTTACCTATTATGGTTTTCTGACTTCATTATTTGCTTCAGCTTTAATTTCAGTAATTAATGAATTTGGTAAACGCCGTGATAGCCGTAGATTGTTAGAATTTCAAAATGAAAACGAGCGATTATTGCATGAATTTTCTGAGCAAATTAAAGTAGATATTGTCCAAGAATTAATTAAAAATAATGTTATTCAGGATCCAGAAGAAATTAAACGAATTGAAGATCTTCAAAATCAAGAACGTGAAGCTTTAAAAATGAAAGCGAAACGCCGAAAGAAAATTTATTTTAGTGATGATTTATTTGATGATACAAAACGAATTAGTGTTTCAAACGTCAAAGTACGTAGTGCTTCAAATGTAACAAAAGTATTAGGTCGATCATCAACAAAAAAATAATTAAAAACCTGCAAGGTTAAATTGCTGGTTTTTAATTTTATTTTCAATATTTACCTCACTCAATGTCTTTTTGTTTTCACTGAGGATCATCTCAAAAAGTAAAAAAAGCGTTTGATGTGTTAAAAGTAATTTCACCAATATACAAGCGATGATCAACATAATAAAAATCAATACGAAAGTGTTTAAAATCATTTTTTAAAACTTCATAAATTTTTTGGCAAACATCTTTTATTTCACTAAAGTCAAAATTAATTATTTCATGATTTAAAATTGATTTATTATTTTTTAAATCAAAAATATTACTTTTTAAAAAATCCCAATTTCAATTATTTTTTTCAATTTCATCCTTATTCATATATAAACAAAATTGCTCTTGTGCAAAACAAAATATTTTATAATCAGCAACGAGTGTTTGTTTTCCTAATAAAGGTTCAATAAGTACTTGTGGTTTAATTTGTGAATAACATCATGTTTCATAAAAATTACGTGGATGATTAATTTTTTTATTAAGCAAGATGTTATTAAAATGAGTTATTATTTGTGTTAATTCTGCTTGTGAAGTAGTTTCACGAATAATATATAAATCTTCATTTTCAGTGCTATTATTCAATTTAATAATATAAGGTTTAGGGAACTTATTAAGATCTAATAAAGTAATTTGATTTTGACGATTAAAAATTGCTAAAGTTGGAACTTGATATTGATATAATCGATGTTTTATTAATCATTTTTTAAAACTTATTTTATCGACAATTTCTAAAATTTTCGATTGATCTGTATTTAAATACATATAAAGTAGTTTTTCATTATAAGTTTTTGGGTCTTGCAAATTCGGAGTATAACCCATTGTTTTGCTTCATATATTTATTAAAGATTTTTTAAATTGATTACGATCATTTTTATAATATAATCTTGTAATTTCATTTAATTCTTTAATTAATTTATCAGTTATCATATTTTTTATTCCTTGATTAAAACATTAACTCTCTTAGTAATTATTTTAGGAGCATATGATAATTTTGAAGCACTTATATTATCAGACCACGCCCCATCTTGTCGATCTATCAATCAATTATTAATATTATTAATTATTAAATTTTGTGATAATAAATATTGATACATACCTCTAATTTCACGATCTGTTTGCTCAATAAGAATTTCTGCAATATTTGGACGAGTATAAACTAATGTAAATCCAACTATTTTATTTATTGATTTTTTTATTAAAAGTGATCCCATAAAATAAGGATTAGTTTGTGTGTTTAATAACAAATCAGAATTAGAATTAACTAGCATTCTTTTTGAATTAATAAAATTTTTAACATCTCATTCTTTTAAGAAAGTGCAAACCTCATGTAAATGAAATTTAGGATTATACTTAATAATTTCATAATCATTTTTAAAATTTTTAATAAAAAAATTAAGATTATTACGCTTTTTTTGTAGAATTTTTCCACAAAAGTATTTTAATGATTGCGTTTCATAAATAAAATTTGAATATCAACTATAAATTAATTCAAAATTAAATTGAGATAAATTATTTAAATAATTTTCATCTTGTATTCCATTAATTGCTAATAAAAATTCTTTTTTAAAATTTGTTTTTAATGCTTCTAAAGCAATTGGAATCAATGTTGTTCAATCATATTTTTTTGCATCAAAATAAGCGAATATAATAAAAAATTTGCCAATAAACTGTGTTGCAATTTTATTTTGTTCAATTATCTTTAATTCTTCATTTGCTTGTGCATAAAATAGAACAATATTTTTATCTTTTAAATACTCAAAATAAATATCATAACCATAATAAGTTCATAATAATAATGTCAAGGTCGAAATTTGTTGTCCAGGATTAATATCTTCTTTTATTTGCTCAACAAATTCATAATTATTTCATGTTAGTTTTGTATACATATAACCTTATAAGATATAATAAAAATCCATATAAAATGGATTTTTAAATGTTTAATAATTAAAAATAAATTAACGTTTTGTAAATTGAGGAGCACGACGTGCACCATATAGCCCAAATTTCTTACGTTCTTTAACACGTGAATCACGTGTTACTAAACCAGCTTTACGTAAATCGGTTTTTAAATCTTCGCGAGTTTGAATTAAAGCACGAGTAATTCCTAAACGAATTGCTCCAGCTTGTCCATTAAATCCACCACCAATAACTTTAACATAAACATCATAAGTTTCAGCTGTTTTTGTTAAAACCAATGGTTGCATCATGTCTTGGATAACTAATTTATTTGGAAAATAATTTTCTGGTTGACGATCATTAACAAGAACCTTTCCAGAACCTGGAACTAATTTAACTCTTGCAATTGAAGATTTACGACGACCTAAACCTTTGTATTCAACAATATTACTTTTTTGCATAATAATTTAATTCCTTCTTCTATTTTAATTTTAATTCCAAAATAGTTGGTTGTTGTGCTTCATGGGGATGTTTATCATTTTTATAAATGAATAATTTGTTTAGAATTTGTTTTGCTAATTTATTTTTTGGAAGCATTCCTTTAACTGAACGACGAATAAGTTCATCTGAATATTTGCTAATCATTTCTTCCCCACTACGTGTTCTTAAACCACCAATATAGTGTGAATGATTATATCAATTTTCACGTAATGCTTTTTGACCAGTTAAAACAACTTTATCTGAATTGATAACTACCACATAATCACCTGCATCAACGTTTGGTGTATAATCAACTTTATTCTTTCCTCTTAAAATATCTGCTACTTTAACAGATAAACGACCTAAAACTAAATCTGTTGCATCAACTAAATATCATTGTCGACGCGCAATAGCGACTTCTTTCTTAAGCATTGAAGACTTTTGCATTTTTTCTCTCCTTAAAAAAAGTTCTTATTTCTGTATAAATAAGTTTTAAAAATTAAAAATATTGGATTTTTTAGATGTAACTAAATATTATTATACTAAAAAGTTAACAAAATAATATAGAATAAAAATCAAATCTTTTATATTATTTAAAATAATCACTCATTAATGTTTCTTTTTTTAAAAAAACTTTTATATTTTTACAATTTATGGTAAATTAATTATTTTTTATATTTTTGATATATAGATTATCGTAAATTCCAAATTTTATCGTTAAATAATATTAGGAGTCTATAAAAGGAATCTATAAAAAATTATGAAAAGAAAGAATAAAATAATAACATCATCATTAGCTTTATTGTTTACAGGAGTAATAGCAACGATAAGTATTGCCGCTTGCTCAAAACAAAATAATCAAAAAACAAAAAATTTTAATTCACAAGAACAAGCAAATAAGATTATTGAGGCTATATTAAATAAAAAAGAAGGTAAGGATAATTTTATCGATTGAGTTCATTGATGAAATAGTCCATTTCAACAAGCACGCAAACTTGCAAAAGATTTAGATAAATATATTGATTTAACAAAATCTAAAAATTTTAAATCAATGATTGATAAAAATGAATATAAGTCAACTTTTAGTGCACAAATTGAAAATGTAATTAGGATCATTAAAGATGATATTAAAATGTATGCTAATTCACCATTTTGAAAAAAATGACGCCAGCAAAAGAAAACAGCACTATTTTTAATTAATTATGCACCTATTCAAAAAGATGATAGCCAAATTAATGCTCCAGGTATTTTATTACCTGCTGAATACCCATTATTATATTCTAAACCTGATTTTGAAGAACTACCAGGATTCGGTGCATTTTTTCCTACACCAAAATCTATGGATGCAGTCGATCTTTTAGATACTTGAAAATCGTTTGGATCATTAATTTCATCTAAAGGTAATTCTGTTGAAAAAAGCGTTTTAGCATCTAAATTACAAAATTCATTTGCAAAAACAGCAGATAAAGTTGTTTACATTTATGATGATGCTATTGTCCCTAATATTTATGATAAGAATGGCAATCGTAATTTAAAAATTACACAAGAATTTGCTAATTGAATGATTAACCAAAATTATAAAGGTTATTTAGCTCGTGAATTTTTAAAAATAAATCAAAAAAATGATTTAATTCCTTTACCAATGAGTATTGTTTGACACGCAAGTTATGGTATCATCGGAATGCATCGTATGTTATATACTTTATCAAAAGCTTTTGGAATGCCAAAAGATGAATTAGAACATTTGAAAGCACAAGAAAAATTTAAAATTCCAACTTTAAAAAAATTACTAACAAATGATGAATTAGAATTGAAAAATGGTATACAAACGATTAAAACAGGCATTGATTCGCCATTTAAATATCATCGCGATCAAAACCGTGATGATTGAAAAATTTGAGCAACTAATTCCCAAGTTTTAGATATTTGTATTGCTTTGGGTTTAAAACCAGATTTATTAGTTAAAGGCGAACTATCAACTAGTGTCCATGAAGATCCTAACTTAGCTTATTATTTAAATGATGTAATAAAAAACCAATTAAGTGATATAGAACAAATTAGTATTAAAAAAAACCACATTAATTGAACAGCAACAAATTATGAACCTATTAAAAATTTAAATGTTAATTTAATTTTAATGGGTGTGCATGGCTTAATTAAAAATTCAGCTTTTAAAAAAATGATGGATGAAGGTAAACAAATTAAAAATTGGGCCTTTACTGACCGCAGATTTAGCGATGAAACACGCCAATATGTTAAAAAAGGTGAAGAAGATTTATATAGCCAAAACGCATCAATTCTTGGTTGAGATGAGTTTTTAAAAACGAAGAACAATCAATAATAATTCTTACTTTAAAGTTAATAATTATTCAATTAAACTAATAAATATTTTAAAAACAGTATTAAAAATATTTTTTTAGAAAATTATTTTTTTTTAATTTCCTATTGTATAATATAAAAATAAAATTTTAAATTTTTTAAAAAAGAGGTCGTTAAAATGCGTCCTCTTTTTTAAAAGAATGATAAGGTTGTATCATGAAGATTAGTGTTAAAAAAACAAAGCAAATAGGTTTGTTTACATCTATTGCAATTATGATTAGTAGCGTTGTGGGAATTGGTATTTTTTTTAAAAATGGATCTATTTTTCGGTTTAATAACTTTAATGAAATAGGAATTATTATTTCTTGAGTGGTGGCAAGTTTAATCGCCTTTTTTACAGCATTAAGTTTTGCATATATTACCTTTTCTAAAAAATCTGGTTCTGGAATAGCTGGTATAATAGATGAATTAAAAGCTCCTAAATGTGCTCGTTTTATTTCTGTTTTGCAAACTTTTTTTTATAATGGAATACTGATGCCTTCCATTTCCTTTTTCGCTGCAGAATCATTATTAATGACAATAATACCTAAAAATTCATCATCACCACAAATATATCAAATTTTTATTTTGGCTATCGGGTTGTTTTTATTTTTTTTGTTGCTTAATTTTATTTCATTTAAATTTAGTTCAATTTTACAAAACATTGCAACAATTATTAAATTTATTCCTATTATTGCTATTGCTATTATTGGTATTACATATGGTATCAACAATGCACAAAATTCATTGTTCAATCATAATAATCCTAAGCATGCGAATTTTAGTCTTTTAGGAATATTAGCTTCTCTTCCTTCGATCTTATTCTCATTCGATTCTTTTTTAGGCATTTCATCATTACAACACAAAATTAAAAATGCTCAAAAAAATGTTCCAATTACATTAATTGTTGGTATGTTTTTAGTCGCTATTGTTTATATTCTAATAACTATAGGGCAAGTTTTTGTAGGCGAAGGTTATGCTTATGGTGTAATTAATAAAGTATTTAAAAATAATATCAATCTCCTAAAAATTATTACTATTATTGTTAATGTTTTTATTACAATTTCAATTATTGGTGTTTTAAATTCATTTGTTATTTTTTCAATTCATAATGCTCAATATGTAATTAATGAAAAAATTATTTTTTGATGATCTTGAATCCAACGTGTTCAATCAAAACATTTTAAAGAAGCACAAGGATTAATCAGTGTTTTATTCATTTTTTTTACATGAATAACCATTTTTATGATAATTAGTATTCCATTAAATACAGACGCGTATATTGATTTATTATCTAATTATCCAATTGTTTTCTTTTTTGCAGTTTATGGTTTAATAATTGCTTTTGCCTTTTATAAAAAAGTTAAAACAAAGCAAAAAATAAAAGGCATTAAAAAACAATATTATACAAGTGATGTTACAAAACGTCCTAAATGATTTTTTAATTATCAAAATAGTAGTTTAAAAAATAAGAATATTCTTAATAAGGAATTAAAAATTGCTAATAATTGAATTTTAAGTGTTTGAATAATTGGCATCACAAGTTGTTTATTTGTTTTTTTATACCAATTTTTATATGGATATACAATTTATGCTTGATTAGATCCTTATAATTTAAAACTATATACTTTTGGATTATTTTATGCAAATAATGTTAGTGTCATTCCATGAATCGTTAGTTTGTTATTTTTTGTTATGCTTATATTTTTCATAGGATTTCCATTTTTAAATGATGCTATTTTAAATCTTAATCATAAAAAAGTAAACAAACAGCAAAAAGTTTTTGTTTAAATGATTTATTGATTTAATATTTATTAATGAGGTATAATTGAATATTATGATTAAAAAAATAATACTAGTTTGCGAAATGTGCATGAGTCGAAATTATCAAACAACACGTAATAAATTTGCTATCTCACGACTTGAATTGAATAAATATTGTAAAAAATGCAATATGAAAACACTGCATAAGGAAACAAGATAAATAATGGTTAATGACAAAAAACAAAAATCAAATAAATATTTAACGTACCAAGATCAAGAATATGAACGTAATCTTTTATTAGAAAAAGAACGTAAACAACAAAAAAAGATTTTGGCACAACAAGCATATCAACAATTAGATCTTAATAAAAAATTAATTAAAAAAGCTAAAAATGATCAAAAACTTATTTTAAGACTACTAAAAGAAGATAAATCAATTTCAAAAACACAATTTAATACCCACCGTTTAGAATTAAAAAATACAATTGATCAAATGGTTGATGAATATTATGAATTATTAGAACAATATAGTGTAGATTTTGAAAAAATAAGTTTTAAATTAAAACGTTGGCTCTATGGCATTAATAAAGAAATCAGACGTACTACTTGGGCTTCTAAACGTTCTGTTATTATCAGTTTAATCATTGTAATTGTTATTGTTTTAATTCTTGCTGCAATATTTTTTGGTATTGATACAGGTTTTTATAAATTATCAGCAAAATAAAATTTTAAAAGTAAAGGAAAAGATTATGGCATATAAGATTAAAGATTTAGATTCTAAATTATTAAGTGATTTAAAAATTGATTTTAATCACAGACATCAATGATACATTGTTACGGTAGTAAGCGGTAATGAACAAAAAGTTATTGAAAATATTAAAGATAAATTAAATGGCTATGGTTATGGTGATAAATTATCAGATTTAAAAATTATTAAAGAAAAAATTAAAGAAGTTAAAATTTATGAGCCATCAGAAGCGCCACGGTCAATGAAGAATCGTGCAAACACAAAATGAGAAACAATAGTTGTTGATGGCGCAACAAAATATCGTTGTACAAAAATAAAAGAAGGTAATAAATTTAATGGATATATCTTTTTAAAAGCGGAAATGACCGATCAAATTTGGTTTTTAATTCGTAATACCCAAATGGTTACTGGTTTAGTTGGTTCATCTGGAAAAAATGTTAAACCAATTCCTGTACCTGAAGATAAGATCTTAAAATTAATTGCTGATAATGATGCTAAACGTGCTTTAATAAGTCTAAATGAAAAAGTTGACCATGAAGAAAATATTGAAGTTATTGAATTGCATGAAGACGAAAATTTTACGAACTTTGAAGTTGATCAAGAAGTTAAAATTATCGCAGATACTTTTTTTGGTGAAATTGCAAAAATTGCAAAAATTGATCAAAACAAGAAAATTGCCACTGTAGAATTTGAATTTTTTGGCCGAATTAACACACTAGATTTAAATTTTAATGATATTCAACTTTATGATGAAGAAAGTGATAAGTTAGAAAACTAATAAAAATAAAAAAACACTTTACATTTTTGTTTGTAAGGTGTTTTTTATTAAAATGTTTTTAGAAAAACTCATCCTATTTATATGAAAGTAATTAATTGAAAAGGGAAAAATGGAATACTACAACTTGTATTTATTATTTAAAAAAGAACTTTGTAAGGAATTGTAAAAAAGAATATTGATTTTAAAAATAAAATTTTTAAAATCTTATAGTTAAATACTTATTATAATAATAAAAAATAAAGGTATTTATGAAATATAAATAGTTTTTATCTTGTTTGTTTTTAAATCTTCAAACTAAATTAAAAGATTTATAACAATAAATGAGAATTCTAAAGAAAAAAGTTTTTTGTTAAAATAGAGACTAAAATATATTAACGTAATTAATTATCGAAATGTTTATTATCATAAACAAAAGTCGATGCATATAAGTTTTACGAGCCTTTAAAACTTATATGCATTACTATTATATTATTTTTTTAAAATTTATTAACTATTTTTTAATTTATATCCACGAATTCGGTGAGCATTCATTTTCTTTTCGATATGAGAGCGTTTGTTTTTTTGATTAATTTTTTGAATAGCTTCTTTAATTTTCTTTTTATATCCTGGTTTTATTTTTGTACTTGTTGTTGAGATTATTTTTTGAATTTCATTTTGTGTTTTTAAATCTTTAAATTGATTTTTTTTCTTAAAAAGAAATTCTTTATCAAATAACTCATTTTCTTTAATGAATTTATGGTGAAAGACGATTCCTTTTTTTGATAAATTCAATAACTTATCATGTTCTTTTTGATCATATAAAACATATGATTCTCCACTATATTTACCACGACCACTACGTCCTGCACGGTGAATGTATCATTCATTTTCAGTAGGCAAATTTCAATTAATAATATGACTTACACCATCAATATCTAAACCACGACTTGCAATATCTGAGGCAACAACATACTGAAATTTATTATTTTTAATATCACGATAGTTGTTTTTTCGTTCACGTTGAAGTAGTGAGCCATGCAAAATGCAAACATTTTTATTTTGTTGAATTAAATATTGATAAATTGCTTCAACTTCTTTTTTTGTATTTGCAAAAATGATACATAAATAAGGTTTAATAGTCTTCATAATTATAGATAATGAATGTCATTTGTCGTGATTATGAATAACATAATGGGTTATTTTTTTATTTTCATAAATTGAATTACTAATATTAATAATCTTTGTATTTTTAAAATATTTTTTTAATTGGATCGATAACATTTCGTGCAGTGTAGCGCTAAAACTTATTTTCTGTACATCTTTATTTGTAAATTGTGAGTTAATAAACTCTAAATCACTAATAAAACCAAGATCCATTAACATATCAGCTTCATCAAAAACAAGTGCATTAACATGGTGTAAATCAAGAATTTGTCGTGTTAAAATTTGTTTTAGACGCGTTGGAGTTGCAATAAGCAGTTGGCTTTTATTTAATTGTGAATTAATTTGCTGGTCAATACTTTCACCACCAATTAACATTTTAACTTGTAAAAGTGGTTGATGTTTTTTAAAAACAATAATTTTACTAAAAATTTGGCGAGCTAACTCACGAGTGGGAGTAATAATTATAACTTGCAGTTTTTGAGATAAATCTAAGTTGTTTAATATAGGCAAAACAAAGGCTAAAGTTTTCCCTGTCCCTGTTGGTGCAACGCCAATAATATTTTCTCTTTTAGCGATTAATGGCATCGTTTTTAATTGAATAGGTGTTGGTTCGAAAATTTTTTGGTTAATTAATGAATCAAGAATTCATTTTTGATAATTACTATTAAATTTCATTTATCGTGCCTCAATATCAGTGTCCATTAATTTGTTTTCTAATTTTATTATTTGCTTATTTTTCTCTTTTTTATCATCATTAATTTCGTTTGTAATTTCTTTTTCTTCATCAACTAAATTTTGAGCTCATTTTGTCTTTTTAAAGATAATAATAGCCATAATTAATTTAGAAATATTTTGGGATTCCATGATAAAAAATAATAATACAACATGTAAACGATCATTATAATCTTCTGGAACATTTATATGTGTTATACATCCCATAATAATCAATCAAATAAAAGTTGATAAACAGTCGATTGTTAATAATCAACGACTACCACCACCACGAATGCAGAAGTAAATAACTGAATATGGTCCTTGTGTCAATACTACCATAATTACAATTCATTCTAAATACATAGCAATTGTCGCAGTGTATGCTGCATGTTTTAAACCTTGTTCTGTATAATCCAACGGTGGATTTAATAATTTATTTACAAACGATGATAAAACTAACAAAATAATTCCCAAACTAATTCCAACTATCATACCCCAATTAATTAATTTGCGCGTGTTTTCCTTAGCTAAATCATGCTTGTTAGCACCTAAATTAGCTAACACTAAAACAGCAACTGTTGATGCGGCACCTGGCCAAATTAAGTTAGTAAATTGAATAATTAAAGCAACAGTTGAAATAGCATCACGATGATCTTGACTATATCTTGTATAAAACATTAATATAATTGTTGTTCCAACTGAAAATAAAATTTCATTTAGTAAAACTTGTCAACCATGAATAAAAATTAATTTAACGACCTTTTTGCTTAATTTCAAATAATTTAAAAAATAATAAGGGTATTGTTTACGAAACAATGTTAGTATTAACATTATTAAAGCATCAATAGATCTTGCAACAATTGTAGCAATAGCCACAAATAAAATTGCCTCAAATGGTGTTACGGCACAATATTTAATCAAAATTGGATCTAAAATAACATTAACAGTTACTGCAATTGAACTTGAAATTAAAGGCATGATTGCATTATTATGTTGACGATAAGCAGTGGCTAATGAAAATGAAAACATGTAAGGAATTAAAGTTAATGATTGTCAACGTAAATAACTAGCTGCTCAATGATTTGTTAAATCATAAATTCGTTTTGCTTCTTCTGAAGCTGAAACATCTAAATTATTTCCTGAATATAATTGAATCATTTGTAATGGTGCTGCCATATAAATAACAATAAATATCATTACAACAATAAAACTAAACAACAACATAAAGTTAATTGTTTCTTTAAATTTTAAATAGTCTTTTTTACCATAATATTGAGCGGCTAAAATCCCCGCTGCTGCTACAATAGAATAGAAAAAAATAGTTGGCAAAAACATTAGTTGTGTCGCTAACACGGTTGCAGTTTTGGCTGCTGTCCCCATATTCTCAGGTGTAAATAAAGCAATGAAAAGACTATCAAAATAATTAACAGACGATAAAATGATAACTTCAAGAATTGCTGGGAAAAATAATTTAGCAGCTGTTTTCATGAAAATTTTATCACCAAATTGATTTATAAACCAATTTGGTTTTATTTTCTGTGATTTTTTAAAAAAATCGAAGCGCATATGTTTCTCCTTTAAAATACTTTTAGTTGCTTTCTAAAAGTTAGATGAATGAGTTTTTATTTAGCTTCAATAAATAATCTATAGTAACTTTTTAAACAATCAATTTTTTAATTTAATTAAATGTTTTGAATTAATTTTTAAAAGATTATCACGAAAAACTCATTATAATATTTGAAATGTGATAGTCTTTTTGGTGGTAAATTAAGAATAATTATAAATAAGCACCTATAATCAAAAAAGTATCATTTGTTTGAAAACTTATTATATTACAACCACGTTCATCCTTAAATCAAGCAGAAATGTTTTTAAATATAACGTTTCAATAATATAAATAAAAAATTTTAAAAGAATGACACTAGTATATAAAAATGATAGCAAAAATTAACGACCTTTTTAAAAGAAAAGATGGTTAATTTTTATATTTTATAAAAATATATAATCTTCCTTCAATTGAAATTAAGATAATATTTAATTTATTTTTTATTGTAATTAAATCTAAATTTAAAACTGTTTTTTCTTATTTCAAATTGATTACTACCTATTAAGTTTTTAGCTCAATTTACCTTTGTAAACAATATGCTTACAAGAATCATCTTTAATAATCAATTTGATTCAAGCAAAGCAAATGTTAGTAGATGTGGGATTTTAGGATTATAAGCAAATGTACTAATTAAAGCAAACATCATTACTCATATAATCATCACCATAGTATCATTAATTGCTCCATAAATAACTCCTGCTCCACGAATGCAATTATTCATAGCAGCATATGGCCCTAAAATAATAATTTCAACAACTAAAACTCATTCTAATTGAGTAGCTAATTTTGCCACTTTCTTAGCATCTTTAATATCTAATAACGAATTTGATAATGGAGGGTTTAATAAAGGATTAATAAATAAAGATAATATAAATAATATAATTGCTAAAATACTAGCTATAATAAGTGATCAAGCGACTGTATATCGTGAATTTTGTTTAGCAGTATTAATTTCGTTTTTTCCTAAATAAGAAAGAACAAGAACTGCAACAATAGTGTTCATTCCTGGTCAAATTAGTTTGGTAAATTGAGCTATTAATAAAGCTGTTGAGATAGAAGAGCGAATGTCAGGATGATATTTATATAAAAATAGTGTTAATAAGATTACTGAAATTGAAAATATAATTTCATTAATAGCAATGAACCACCCGCGTTTAAAAATATTTTTTAATAATCTTCATGTTAAATTTCAATAATTAATAACATATGTTGCCCTTTTTTTAAAGATACAAACACAAAGCAAAACAATTAATAAATCAAAACCACGAGAAGCGATTGTTGAGAATGCTGCAAGTAATATCGCTTGTTTAGGAGTTTTGGCAACTAAAATCATTAATACTGGATCTAAAATACAATTTAGACAAAGTGCGGCAATACTTCCTAATAAAGGAATTGCTGGACGTGCATCTTGACGGTGTGCAATTGCAAAAGGATATGAAAAAGCATAAAAAACTTGTCCAATCATTGCGTATTGTAAATACATAGATGCTCATTCATTAACTTTTTTAAAATTAGTTAACTCATTAGATGTGACCATTGACATATCTTTACTAAAATTATTAATTGATCCTGCATATATTGTTATAGATCAATGTGGAAAAGTTAAATAAATAATTAAAAAAGGGATATTTAATATAACAGATCCTAAAATCATGTAATTTGTTGTTTCTTTAAATTTATGATAATTTTTATTTCCTAAATATTGTGAGCTATAAACACTGCATGCATAACAAACAGCAACGGTAAAAATTGTTGGTAAATATAATAATTGAGTTGCAAGAATTGTTGCTGTTTTAGCATCTAATCCTTGTGATTGAGGGGTATATGTTGCTAGAAAAAAATTATCAACATAAGCAATTGCTGTAATGATAATATTTTGGATAATCGCTGGAAAAAATAATTTTAATGCTTTCTTGATAAATTGTTTATCGCCAAATATTTTTTTAAAATTCAAAAAAGACAATTTTTTTTGTTTAAAACCAATTTGATAAGACAATAAAAATCACTACTTCCATTCTTCTATAACGATAATAATATAATAATTAATTATAGTGTAATTTTTGGAATAATAAATTTAAATTTTACTTCCAGTATTTATTAAGTAAACAAAAAAAGGTTTAGATGCAAAACCTTCTTTTTGTTTTTTAGTGATTATTTAATATTTTAATTGCTGATGACGTTCCGATCCTTGTAGCTCCAGCTTCAATAAAAGATTTAAAATCCTCAAAATTTTTAATTCCACCTGATGCTTTAATTTGTTTTACATCACCTAAAATTCGCTTTCATAGAATAATATCTTCAAGTTTTGCACCTTCTTTTGCAAAACCAGTTGATGTTTTAATGAAATCAGCATTTGATTCTAGAATAATTTTAGCTGCAAATTCTTTTTGCTCCCTGTTCAAAAATGCTGTTTCAACGATCACTTTTAAAATTTTATCCCCACAAACTTCTTTAATTTGATTAATTTCATTTAAGCAATATTCTCTATTACCATTGATTAATTGATTAATATTAATAACCATATCAATTTCATCAGCACCTAAATCTATAGCGTTTTTTGTTTCAAAAACTTTAGTTTCAATTGTGTTAGCGCCTAGTGGAAAACCAATAACTGTGCAAATTTTAACAGACGTTTCTAATAAATAGTGTTTTGCAACTTTAACAAAACAAGGATTAATACAAACTGAATAAAATTCATTTTCTTCTGCTTCTTCACACAACTTATGAATCTCTTCTAAACTTGCGTCAGCTTTTAATAATGTGTGATCTACATAAATATTATACTTATTCATGATATTCCTTATTATTGGTTTAAAAGTTGATTAATAATATTTTTAACACTAAAACCAAATTCATTCATTAATTTATCACCATCTGCACTACGCCCAAATTGATTAGCAGCAATTTGTATAAATTTATTTGTATAAATTGATAATTTTCATCAAAGCATTTCGCTTGATGCTTCAATTGCTAATAGACCATTTTTTGAAGCTAAAAGGCTCTTAATATCTTCAGGTTTTTGACGTAAAAATAAATTTAAGTTAAAAGCAGAAATAACTTTAATTTTAAGTTTATGTTTTTCAAAAAGTTCTTTTGCTGCGTCATTTGCTAAACTAACTTCACTACCACTAGCAATAATCGTGTAATCTACATCTTTACAAAAAGAAAAAGGCGAATTAATAATATATGCTCCCTTACTAAAATCTTGTGGTTTTTGATCATTAATTTGTTTAATAGTTTGACGTGAAGTAATTAAACAAATCGGTTTTTGTTTTTGTTTAAAACTATATGAAAGAGCATATTTAACTTCATAATGATCAGCAGGACGAATTACTTCTACATTTGGAATTGCTCTTAACATTGGTAATTGATCGACAGGTTGATGTGTAGGTCCATCACCACCCACTGCATAACTATCGTGACTAAAAATAAATAAATTAGCTAAATTACTAATGGCAGCTAAACGAATTGCTGGTTTCATATAATCAGAAAAAGCTAAAAAAGTGCCTCCTATCGCTTTAACGCCTTGATGTAAACTTATTCCATTCATCGCTCCTGCCATAGCAAATTCACGAATACCGAATTTAATATATGGATTTTTATGGTTATCGTTAAAAGCGCCTTCGCCTATTTTTGTGAAAGTTGATTTAGCTAAATCAGCAGATAAAACAAGAGCTGATTTTAAATCTTTTAATTGATTAAAATATTGTTTTAAATAACTGCGAGTAGAATCATTTAAATTAGTAATTTTATTTTCATCTAATAATTTATTTAAATCTTCATAATTACCATTAATATAATTTAATAAACGTTGCATTTGTTCTGGATTAGTTTGCATATATTGGTCAACTAATTGTTGTCATTGATTATAAGCACTTTCGCCCCGAGCTACAACATTAAAAAAGAAGTGATCAAAAATTTCCTGATGAAATTCAAAATTATTTGTTTTAATATGAAAACGCTTAGAAAATTTTTCTAATTCATCATTAGAAATTGCTGCAGCATGCGCTTCATTACTATTTTCAAAAGATGTCCCCTCACCAATGATTGTTTTGACTTCAATAAACGTTGGTTTAACATTCTTTTTTCATTTAGCTTCAGCAATTGCTTTGAAAATATTTTCAGGATTATTATCTGTTTTTAGATAATTTCAACCCATTGATTCAACACGCATTTTTAAATCTTCAATATTAACATCACTTACTGCTGAATCTAATTGGTAATCATTTGAATCATGTAAAATAATTAATTTCGATAATTTTAATTTTCCAGCAATACTCATTGCCTCATAACAAATTCCCTCTTGTAGGTCCCCATCGCCAACAATACAATAAGTATAATGATCAATAACACCTTTAAGAGTAGCAAATTCAGTTCTTAAATAACTTTCAGTAATTGCCATACCAACTGCATTAGCAACCCCTTGCCCTAGTGGTCCTGTTGATGCATCAATATAATTATTAGCCAACACTTCAGGATGACCTGGTGTTAAATAATTATCATTGCGAAAATTTTTAATGTCATCTAATGTTAATAATGATGAAAAATAAAAAACAGGATATAATGCCATCGATCCATGACCAGCTGATAAAACCAAACGATCGCGATTAAATCACTTTGGATGTGATTTTGAAATAGTCATTAATCCCTTATAAAGGGTATAAACAATAGGTGCAGCTGAAATACTCATTCCACTGTGACCTTGCTTTGCTTTATTAATAGCCTGTAAAGCAAGTGAACGCATTGCGTTAACATAACGATTCATATTTAATAGTCTCCACTAAATAAAGATATTTAAATTAATTTCTGTTCAATTAAAATTTCTTCTAATTTGTTAATTGCTTTATTTGCATCATTTCCAACGGCACTCAGCTCGATTTTTGTGCCTTGTTTAATTCCTAAAGCCATAATGTTGATAATTGATTTAGCATCTGCTTGACGCCCATTTGCTTTAATAGTAATTGAAGAGCTAAAATCACTAGCTGCATTAACAAAATAAGTGATCGCCTTAATAAATGCTGACAATTCTGCTACGATGGTAAATTCTTTCGATATCATATTAAACCTTTACATAAAATATCATTTTTTAAATGTATTTTAATTATATTACTTAAAGCATTGTCTTGATATCTTATATTATTAATATAATAACTTTTATTATAAAAGTAATTGAAAAATATTAATAAATACTGTTTGAACTAATTCGTTGAATGTGAATTTGTTATCTAAATCTTCTAAATTAATTAACTGTGAATTACGGAGATATTGATTAAAAATCTCGTTAATGTCGTGATTTACTTGTATTGAATAAATTGTTAATAATGATTCGTAATTAATTACTAATGCTCTATTATCAAGATTATAAGAACCTAATAGTGAAATATCATCATCTATTAAAATCGCTTTAGTGTGTAAAAAACCACTATATTCATAAATTTGTACACCGGACTTAATTAATGATTCATAACTTAAACGATTCATTGATATGATAAAACTTCAAGAATCAACCCTTCCAGGTAAAATAATTTTAACTTTTATTCCAGTATTCGCTGCAGCTTTAAGTCCTATTAAAATATCTTCAGTAGGAAATAAATATGGCGTAAAAATTGTAATTGATTTTTTAGCATTAGCGATTGCCATAACCAGAATTTCATTTAAAGAACTTGTTGATTGGTCTGGTCCTGTATGCAAAAGTGTTGTTATTGTTTTATTTTTAATAGAATTATTTATTAAGTCATCTTTTCCTTGACATAAGTAAGGTTGAGGATGCATACTGTCAACAACAACATTAAAATTATTCTTTTTTGAAAATGGTGTATAGTAGTATCAATCATGAATAAATTCTAAAAGAATTGTATGCACAATTCCTCCGGTTAAAATATAATTCAAATCAACTCAATGACCAACCTTAGGATCAATATTTAAATAAGAATCACCAATATTAGAGCCGCCACTAATAGCAACTTCACCATCAATAATTAAATATTTTCGATGCGAACGAAAATTAATAGTACTTCTAAATTTATAATCTGCACGTGCTTTAAAACAAGCAATTTCTATACCATTATTAATTAACTGTTGACGATATTTTTTAGGAAATTTATTTAACGAACCAATTGGGTCATATAATATATATATTTTTATACCATCATTACTTTTTTTTATTAATTCTTTAACAAGAGATTGTAATCAACGACCATTCTCAATAATATAATATTGTAAATAAATTGTTTTTTTTGCTTTTCTTATTAATTTAATAGTTTGTTCATACATTTCGTTTGTCTCTTTAATAATTGTTATTTGATTATCACAATAAATAGGTGTACGAAAAAGTTGGTAATTATACTGACCAATTTTACGAATTAAATTAAGTCCATTATTATTAATAATCATATTTTGAAAACTATGATAAAATTCATAATCTTCAAATTTAAAAAGTGCACGACGGTTTGTAATGATATCATTAATTTTTTTATCTTTAAATGGGCGTGATCCAAAAATCCAAAAGAATATAATTGACACAATTGGTAGAAATAAAAAAATAAACAATCATGATTTTTTAGAACGATCTTGCCGATGTCTTGAAGATAAAATATATAAGCCAATACTAGTATTTAAAATATAAAATGATATTAAAATATCTGTAACAATAACCGTTGGTCGATGGTGAAAATATATTGATATTAAACAAATATTAATTATTAATAATACACAAATAAATGTTATGATGAATTTGGCTAAAATACGTTTTTTCATACTTATTATCGTCCTTTTTATTAATAATTATAATTTATATAAATAAAAAATAGGGTTTCCCCTATTTTTAATTATTAATGGCGGAAACAGTGGGATTCGAACCCACGCGCCGCAGAACGACCTAACACCTTAGCAGGGTGCCCTCTTAACCACTTGAGTATGTTTCCAAAACAATTATTGCTTTATGTATATAACTTATTTATTATATTAAATAATAAGCAATAATGCAATAGTTAAAACTAATGAATTTAAATTATTAAATTGATCGTAATTTATTAACTAAAGAATTTTCATCAATAAATCCTAAACTTGTCGCTTTAATATCTCCATTTTTTAAGTAAAATAAAGCAGGGATTGATTTGACTTGATATTGTGTTGATAATTCTGGAAAATGATCAACATTTATTTTAATAATAGTAAATTCATCACCATGTTTTTTTTCAATTGAATCTAATACTGGAATAAGCATACGACATGGAGGGCATCAATCCGCATAAAAATCAATTAATATTGGTTTAGAATGATTTTCCTTTAATAATTCATTTAAATTTTGGTTACTTTCTAATTTAATTAACATAAATTTTCCTCTTTTTAATATTATATTATTATTGATTAAAATCGTTTTTATCATTGTTTGATTCATCTAAATTACGAATAAAATTACATTCAGGATAACCTGTGCATCCAATAAAATATTTATTTTTACGATTTTTACGTTTAACCAATGATTTTGAACAGTTAGGACATAATTCGTTAAGAATTTCTGGTTTCTCTAACGATTCTGTAAAACGACATGTAGGGAAATTTGAACACGCAATGAACCGAGTGTTCATTCTAGATCTTTTAATCAATAAATCATGAGCACATATAGGACATAATCGATTAATTTTTTCATCAGGAACTTTTTCGATTTTAGTGTATGCTTGTTTTACTTCTTCTTCAAAAATAGGAATAAAAGTGCTAACTAAATCTTTTCAAAATAATTTGCCATTAGCTATAACATCTAAACGTTCTTCCATCTCAGATGTAAATTTAATAGAAATCACTTTTGGAAAGGCTTCTTTTAACTCTTGGATGACTTTAATCCCTAATTCTGTTGGAATTAATTTACTCTTATCTTTATTGATGTAACCCCTAGTGGTGCCAATAGTTGCCATTGTTGCATATGTTGAAGGCCGACCTACTCCGGCATTTTTTAGCGCTGCAATTAAAGAGGCTTCTGTGTAATAAGGCGGTGGCGTAGTTTCTTTTTGTATTAAATCGATCTTTTCTTTTTTAAATTCATCACCAACATTAATTATAGGAGGTTGGAATTCTAATTCATCACGTTGCTTTTTATAAAAATCTAAAATTCAATATCCTAAAGTTCGAATTGTTTTATAAGCTGTATAAAATTTATATTCATTATTAATAAAACGAATTACTTGCTTATTAAAAGTTGGCACTTTCATTAAAGCTGATAGTGTTTTATTTCAAACTAAGTTATACAGCTTATAAATATCTAATGAAACATAATCTTTAATTTCTTCAGGTTTTAAATTAATATCAACAGGGCGAATTGCTTCATGTGCGTCTTGAATATTTACTTCTTTTCTATTAATTTTTTTATTTTTAAAATTTTCTAGATATTCTTTACCAAAATTATTTTCAATAAAGGCATTAGCTTCTTGTAAAAAATCATCACTTAATCGCTCGCTATCTGTTCTTGGATAAGTTATTAAAGCTAATTGCTGATTATTAATTTCTAATCCTTCAAACATTTGTTGAGCTATTAAAGTTGTTTTATTAGCACTTCAACCAAAATTATTGGATGCGATTTGTAATAATTTATCAGTTGTTAATGGTAATTGAACATCGCCTTTGGTGATTTTCAAATCATCAATACTATAAACTTTAAAAATATCACTTAAATCATCAATCACACGCTGTGCATCTTTTTTGTGTAGAAATTTTAAATCACTATCGCTATTATCTTTCTGATTATATAATTCAACATCATAGGGAATTTTTCTTAAAGATACAAAAATGTTTTTTTCAATTATTCCTTCAATTTGATATCATTCTTCTTTAACAAAAGAACGTCTTTCTAACTCTCTCTCAACTACAAATAATAAAGCGATCGATTGTACTCGCCCAGCAGATAAGCCATGTAAGTTTTTTTTAACTAATCCAGAAAGTTTATAACCAATAACTCGATCTAAAATTCGTCTTGCAAATTGCGAATCTACCAAATTAAAATCAATATCATGTTTTTCATCAATAGATTTTAAAATAGCTTTCTGTGTTATTTCATTAAAAGTTATTCTTTTAACACGTTCTTTATCTTTTTTTGGTAATAAATCATAAACATGTCAACTAATAGCTTCACCTTCACGATCAGGGTCAGTTGCTAAATAGATATTATTTGATTTTTTTGCTAAATCAATAATTGTTTTTATTTGTTGTTTTTTCGATAATTTTTTATTTGTTGTTTTTATTATTTCTCATTTTAAATCATAAGTTTTTGGATCATAACCAGATTTTTTTGCGAGCTCACGTAAATGTCCACATGTAGCTATTACATTATAACTATCACCTAAATAAGACTGAATTGATTTAATTTTATTTGGTGACTCTACTACTATTAAATCTTTTTGCATTTTTTCTCCCATTTACTACAATTATTTGCTCGTTTTAACAATTGTAAATAATTATAATATTTTATAGTTGTTGTTTAATTTATTAATTTTGAATTTTTAATAAATTGATAAATACGATTAATGTATTTGGGATAAATTGTGTTTTGCTTAGCTAATTTTTTATCTAATAACACCAAATCTAATTGGCAATCTTTATATTTATTAAAAAGATAATCATCTATTTGATATAACTCATTAACACATAAAATATGGTCGCTTGAAGCGCATGCAAGACGTTTTAAATCCTGTGTAATTTTATTACTATTGTAATCTTTTGGAATTTCAGATATTACACTAACATTGCAGTAACTTAAGTATTTAATGTTTTTAGAACTATAGATTCCCTCATCTGTGATGATACATATTCTAGCTTTAAGAGAAATTAAACGATTGATAAGTGTGTCCAAAACAACACTATCATAATAAATAACAAATCCATATCCTGCTTTTATCAAGGTTAGTATATCATAATTTGATATTGCATTTTCATAATCTATTAACCAAATATTTTTTTTATCAAGTCATTCAACTGTTGTTGAATAATATAAAATAAAAGGAGGTTGGTTAATTTTTTTATATTTATTAGGATATTTTTCATCAGTTAAATCAATAGCTTTAACTTTTTCGTAATCTTCTTTTTCTAAAAATAAATATTCATTTTGATCTTCATGATTTATTAATGCTAAATAAATTTTTGATCAATCCCCTTTATACTTCAAACTGAAATAGTGTGTTAAATAATTCAATGGTATCACCTCATTAAATTATTAGCAATAATGATTTATTTTTAAAATTACTAATCATAATTAACTTTACAAATCAATCCATTAGAAAAATCATTAAATAAATTCTCATAAGCTCTTACATGATCAAATTCATTATTTGTTAATTTATAATTATATAACTCACAAATATGATGAATAAAATCATCAAAATTTAATACCTTATTCATTTTATATTTTATAAATAATTCATTCCTATAATTTTTTAAATAAAAATCATATGCAAAATGTACAATTGATTCTAATTCCAAAACTTCTTTCTTAACACAATTAATCAAAGTCAAAACAAAACTATCACGCTCATTTTGAATATTTTTTAAAAAAATTCCAGGTGTATCAATTAAATAAAAATGTTGATTGATTTGACGAATACATTGAGTCTTAGTAACTCCAGGTCTATTTTCAACTTTTAATACTTTTTTGGGAGCTAAAAAATTAATCAAAGAAGACTTACCAATATTTGGTAGACCAATAATCATACCTACAAATTTAGGATTTATTAATCCTTTCTTTTGGTATATTTTTATTTTTTGATCAAATAATTGATATAAATGTTGAATAATCTTATTTTTATCATTTCTTTTTTTAGTTGAAATTAATAAAAAATTATTATCGAAATTTTTTTTTCAATCACTTAAATCGGCCTTATTAGCCAAATTAATAACTGGTTTGGATGAAACTTCTTTTAATAATTCAATATTACTTGATGTTAATGGACATCTAGCATCAACTAATTGAATAAAAAAATCAACATTTTTAAGATTTTTTAAAATCTCATCTGTTGCTTTTTTCATATGCCCAGGAAATCAATTAATTTTTTTATTAATTATGTCCATAATGTTAAATTCTACACATACTCATTAAAAGATATTAAATATTTATAAATTTGTTTTTGTTGATATTGAATTTTTTGTCTTATATTAAATGTATAATAAGCTAATCTAAAAAAAATAAAAGGAAGATTTTTGATCGGTTTTTTCGGTGGGTATTTATTGATTTTTAAAATTAAATATCTTGCTGTGCTATTATGACAATTGATCCATAAAAGATAAATTATTGTAATAAATAACATTAAAAAACTAATACATGTTAAAATTGTAAAATCTAAATATTTTTTATAATTTAATAATTTTTCATTATATCCATAATTTAAAGCTAAAATTAAATTTGTGATAAAAGAAATTAATCAACCAGCTAAAAATATACTAATAAAAACTCCAATAAATTTATATTTAAAATATAATTTACCTATCAAATAATCTTTTAATTCTTCTAAAACTATTTTCAAAACATTTACCTAAATATTAAATAACTATTAATGTAATTATATATTTTTATTTCGTTTTTTATTTTTAAAAAATTTAATAAATTCCTCAGAATTACTATTTAAATAAGGACGATTAGGTACCTTATGATGATGACGACAAACAGCAGAATAGAATTCCTGACAGCCAATTTTAACAATATCATCATTATAGTCAGCGTGTTTACCATCAATCTTACGCAATGAATGCGTTGCTTCAGCGCCACACTCATTACATATCGCTGTTAGTTTTGTAATTTTATCAGCATAAGTAAAAAGTTTAGCAATTGGACCAAAAGGCTCGCCTTTAAAATTTTTATCTAATCCTGAAATAATAACAACAAAACCATTTTCTGCTAAAATATTTGCAACTTCACAAATCCGATCATCAAAAAATTGAACCTCATCAATGCCAATAACTTTTGTTTCATCATTAAAACTGTTTGACATAATATAATTTAAAATTTCTGGTGCGCTTTCTACTTCAACTGATGGTAAAGACGTTCCTGTTCGTGATTGGATATTTCTAATAGATCTAGTATCAATTTTAGGTTTAAAAACCAAATATTTAACATCTGCATATTCTAAACGATGTAAGCGACGAATTAACTCTGCTGTTTTTCCAGCAAACATTGGGCCAGTAATTAATTCTATTCATCCTATTTTTTTTGAAAATGCATTCACTTTAGCCATAAAAAATTATTTTGTCACATTGAATCTAAAATTGCAAATATCGCCATCTTTCATTAAATATGACTTTCCCTCCATTCTTATTTTTCCCGCTTCTTTCACAGCTTGCTCACTTCCTAATGATATTAAATCATCATAATGCATAATTTCAGCTTTAATAAAACCTTTTTCAAAATCGCTATGAATAATTCCTGCACATTCAGGCGCACTCATTCCGTTTATAAATGTTCAAGCACGTACTTCTTGTTTTCCATATGTAAAATAAGTTTTTAAATTTAATAAATCATAGGTTTGCTTTACAATTTCATCTAATCCTGTTGTTTTAATACCTAAATCTTGAATAAATATTGATTTTGATTCTTCATCTAAAATGCTAATTTCATATTCAATTTTTGCTGACAAAGCAACGCATCTAGCATCATTATCTTGAGCATACTTTTTTACTTTTAAATATTCTTTATTTAAATCAGGATTTGAAATCTCATTTTCTGAAATATTTGCAATATATAAAATTGGTTTTAAAGTTATTAAATTGAAAGAATTAATGATTTTCAATTCTTCTTTGGTTAATTTAACACTTGATGCTAATTGACCCTTTTTTAAAGCTTCTTCAATGTTAAATAAAATAAGATATTCTCTTCTAGCATCTTTATCGCCAGAATCAGCCTTTTTTTTAATACGACTAATTCGTTTTTCAATAATATCAAGGTCAGATAATATTAATTCATAATTAATAACATGAATATCAGATAATGCGTCAATTTTATTATGAACATGAGTAATAGATTTATCATCAAAACAGCGAACGACATGACAAATAGCATCAACATCGCGAATATTATTTAAAAATTGGTTTCCTAGTCCTTCACCTTGCGAAGCGCCTTTAACAAGACCTGCAATATCAACAAATTTGAAAGTGGTCGGCACAATTTTATCAGGCACAACTAAGCTTGCTAATCGTTTTAAACGCGGATCATTAATATTAACAATTCCAATATTTGGTTCGATAGTTGCAAAAGGATAATTAGCTGCCTCAACTCTAGAATTTGTAATTGTATTAAATAATGTGGATTTACCAACGTTTGGCAAACCAACAAGACCTGCTGATAACGACATTTTATTCTCCTTATTTTATTTTATTTAAACTATTAAACTTAGTGAATTTATCAATGATTGTATTTTTAATTGCGTCCACACCATAAGCCAAAATTTTTCGTGGATCATACTGTTTAGAAAGAATTAAATCACCTGCTTTTGTTAAGTGATCTTGTAATGCATTTGAAAATGCAATTTGACATTCTGTATTAATATTAATTTTTGCTACACCCAAATTAATTGCTTTTAAAATTTGTTCATCACTAATACCAGAACCTCCGTGTAAAACAATTGGTTTATTCACTTTATTGTTAATTTCTTTTAATAAATTAAAATTTAAACCTTTTCAATTTTTAGGATATATTCCGTGAATATTTCCAATACCACACGCTAACATATCAATATCTAATAAACACATTTGATAACAATCGTCGATATTAGCTAATTCACCTTCTGAAGTAATCCCATCTTCTGTCCCACCAATTGAACCAACTTCAACTTCTACTGATACACCTTTTAATTTAGTTATTGTCAAAAGTTCTTTAGTGTTTTTAATGTTTTCTTCGATCGAAAGTTTTGATCCATCATACATAATTGAGGAATAACCAGCATTAATAGCTTTTATACATCCTTCATATGTACCATGATCTAAATGCAAACAAACAGGAGTTTTAATATTCATTTGTTTTATTAAATTAACAACCATTGCATAACAATTATCATAACCACCCATATATTTTACGGCACCTTCAGATGTCGCTAATAAAAGAGGAGAGTTTGTTTCTTGAGCTGCTAATAATGCTGCTTTAATTCATTCTAAATTATTGATATTAATTGCAGCCACTGCATAATGATTTTCATAAGCATTTTGAATCATTTTTTTGGCATTAACTAATGGTGACATTTTTTTATTCTTCCTCTTCTAATTCCTCTTCGTCTTCTATTTCATCTTTATTAGAAGTATTATTATTTTTAAATTCTTCATCTTCATCTTCGTAGCCAACAAAATCAGATAAAGAGCTTACTTCATCACTTTCCTCTTGTTCTTTTGATTGTGTTAATTTATCATTTTTAACAATTTCATGTTCATCTTCTTCACGGTCACTATCTTTGTATAAACCAACTTCATACATAGCGTTATTAATTTTTGTAATTTCATCTAGTGATAATCGTTCACGTAAATTTCATTCTAAATCTCCAATTGAAATAAAACGTGTATCTTGTATTAAAGTTGTATATAAAACACCTAATTGTGAATTAACTTCTACTAAATTTACATTACTATTCTTGACAATTTCATTAATAATATCATCAAAAGTAAATGGTTTTTTGTTATAAATTTTATTATTTTTTATTGCATTATAAGCAATATCAACTAATTGACGGCTCATATTAAACTCCTTATATTTTAGTTTTTTTGTATTTAATATATTAGTATTTATTTTAAAGGTTTATAGTAAAAAATTCATTTTTTTATTCGTGGTTGTTTAATTGATTTTAAAGCAGCAATTATTAAACTCTTATCTTCAAGTTTTAATAATTTAATATCATTATTTTTGTTTTCTGTATATAAAAAAACCGGTTCATTAAAATCATAAATTTGTTTATTTGAAGTAATAGTTTCATAAAAATATTTTTTATGAATAATCTTTTGATCTTTTGATTTATCTTTACCATATTCATATTCAAAAGTTACAAAATCTTTATTTTGTAACCAATTTGTTGCTAAAGTTTTTAAAATAAAATCATGTTCATTAGCAAACGATCGCATTAAAGCTGTAAATGAATAATCATCTAAATTTTGAACAGTAGTAATATCTCAAGATTCATTATCTAATCATGGTTTTAAAATTGTTAATAATTGTGAATTTTTGAATATATATTTTTTTTGTTTTTGGTATAAAAAATGCACTCTTGCTAAAGTCTTTTTCATTAAAATATCATAGGTTGTTGGTGTTTTATTTAAATAAACATTTTCAAACATACTCCAACGAGAAATTAAAATGTTTTCTAAAGCGTTTCAAGAGTGTTTTGAAAAACATAATTGATCGTTAAAAACTATTGCCTTTTTAAAGATATTATCATAGTCTATATTTCCATACTTGGTTCCTGTAAAATGAGAATCACGTGGCAAATAATCTAAACGATCTGCATCAATTTGAGATGAAATAACCTGTTGCATTCACATTTTTTTGGATTTATGATCAATTATATTAGCAACATCAACTGGATTGATATTATGTTTAATTAAAATTTTATTAATTTCACTTTTAGGATTAGTAATAATCATTTGACCAATTTTTTCATGATTATAATTTGTTGCATATTCAAAAGTATGGGAAAAAGGACCATGACCAATATCATGTAATAAGGCTGCAGCACAAACAATCTTTTTATTATTTATTAAATCGTGTGTATCAGATTTAATGAATTTTAATGAATCTAAAAGTTTTCTTGTAACTTGGAAAACACCAATACAATGAGTAAAACGTGTATGAGTAGCACTTGGGAAAATTGATCAACTTGATCCTAATTGCTTTATATTATTTAAACGCCAGAACTCTTTTGTTTTAATTAATTCATAAGCTCATTCATATTGTTCATCAAACGTAATTAAACCATGAATAGGATCTTTTATAAAAAAATTTTTTACCATAATAATTATTAATCCAAAATTTTTAAAATATTTATTAAATTTTTTAATACTTTTTCGCGTCCTAAATAACAAATTGTTTTAGCTAATTCTGGACCATGCGAACTAAATGTAGTTGCAATACGAATTGGCATGTATAAATTAGCACCTTTGTTATTAGTTATTATTTGAACTTTTTTAATTATTTCTTTTATAAATTCTTCATTAAATTCATCATTAATTATTAATTGTTCATAAAAAACACGAACAACAGTTATTAATTGGTTTTTTTTAATATAATCTACATCTGATGCTAATACTTTTTTATCACAATTAAATTGTTCAATTACTAAATCAATTAGTTGTTTTAAATTAATAATATGAGATTTAAATAATAGACATTTATTAATAAAAGTATAATCATTCAAAACTAATTCTTTAGTTAATGAATGTTTCTTTATAAAATTGATAAATTCTTCTTGGGTAATATTATTAAAATATTGTGTTGAAAACCAATTCATTTTATTGATATCAAAAAAGGCGGGGGATTTTGATAAATTATTGATATCAAAATTTTTAATCATCGTTTCTAAACTCATTATTTCATCGTTATTTTTTGGGCTTCAACCTAATAATGCAACAAAGTTTGTAATAGCATGTGGCCAATAACCATCTTTTTCATAATCAGACACAAACTGTTTTAAAGATAAATTTCGCTTTGATAATTTTTTTCCTGTCTCATCAACAATAATTGACAAATGACCATATTTAATTTTTGTTATATCATAATTTAAGGCCTGAGCAATCGCTAATTGATAAGGTGTATTAGAAATATGCTCTTCTCCACGAATGACATGTGAAATTTGCATTTCATAATCGTCAATTACAACTGCAAAATTATACATTGCAATCTTATTTGATTTTAAAATGACAGGATCAGTTAAAGCACTACCTGGAATACTAATTTTACCCCGAATTAAATCATCCCAGCTATATTCCATATTTTCATTAATTTTTAAACGAATTGTGTATTCTTTATTTTGTAATAAGTTACTTTCAATTGTTTTTTTATCTAAATTTAAACAGTGCCGTTTGTATTTAGGTGTTTGATGTGATTTTTCAGCTAATTCGCGATCTGCATCTAATTGTTCTTTACTACAAAAACAAAAATAAGCTTTTTTTTGATCAACTAGATCTAATGCTAATTTTTCATAATGTTTTAATTTTTCAGATTGGATATATGGGCCAAAAGCTTTAGGATTACGAATTGATTCGTCTGGAATAATACCCATTCATGCTAAAAAATCTAGTTGGGAATTAATTCCCCCTTCCACATTTCTTTCAATATCAGTATCTTCAATACGAATAATAAAATCCCCACCATAAGCTTTTGCTAGTAAATAATTAAATAAAGCTGTTCGCGCTCCTCCAATATGTAAATAACCGGTTGGTGATGGAGCATAACGTGTTCTAATTTTCATTAATAATTAAATCTTCCTTATTTTTTAATTTTATGCGAATAAATGAACTACCTGCACCACTTAAAATTGTATAAAAACCATCGGATAATAATTCATTATATTTGTATTTAATATTAGGGTATAGCTCAAAACAGTATTCTTGTAGATCATTATGTATGTTAACTACAATATTTTCTTTTAAATTTTTAATAATTGTTTTAAAATTATTTTTAATAACATGTCATGAATTATCATCAAATTTTTCAAAAACAATTTTTGTATTTACATTAACATTCATTAAATAAACTTCATAATTTAATTTAAATTGACCAGTCAAATCCTCTAAAACACTTCCGTAATCACTTATATAAGCTGTTTTATAGCCTGATAAAAAAAAGGGAATATCAGCACCTAATTTATTAACAACATCTTTATAATTAATTTCTTTAATACCCTCAAATTCTAGAATATATTTCATAATGGCTGCAGCGTTTGATGAACCACCACCTAAGCCTGCACCAATTGGAATTCTTTTTTTAATATTAATTCGATAATGATTTTTAATATGATAAGTATGACGAATCCATTCTAATGTCTTATGAACTAAACGGCTATAAACATAAATTTCATTGGTTTCATTGTAGTAATGAACATCATCAATATTTTTTTCTATTTTAGTAATTTCAATATCGTCATAAATATTTTCAACAAGTATAAAAATTGATTCAAAATCGTGTTTTGTAAAATTCTTTTGTTTTTTATAAACACTATATCCTAAATCAATTTTTGCGAATGACTTTACTTTCATAATTTGACCTACAACTTAATTTTATTAATTATCTTATCTATTCAAATAATTAAAGAGTTTTAGAAACATTTTTGGTTCAATGTTTTCAGCTCGTAAATTCATATCTAAATTATAATCAATAAACATTTGATTAATTATTTTAATATCATAAATATTAGATAAGTTATTTTTTAACTTTTTTCGTTTATTTAAAAAACAAATCCGTAAAAATTTACCTAATTCTTCAATATTAAAATTCACACTATTATGAAGATTTTCAAGATGAATGACAGCACTTTGAACTTTAGGTTGGGGATGAAATTCTTTAGCATTAACTTCGAATAGAATTTTTGTTTTACAGAATAATTGTACTAAAACAGTAAAAGCATTATAACCACGTGTATTTACTTTAGCACCAATTCGTTCAGCCATTTCTTTTTGAACCATAATATAAGCATCATTAATTAATTTTGATTGAATAATTTTTAAAACTATCTTTGAAGATATTGCATATGGAAGATTTGCAACAACTTTAATTTTTTGAATTTTTTGAGTATTATTGTAATCTAAAATCAAATTATCTAAATCTACACATAATACATCATTATTTATTATATGAAAATTACTTGTTTTTATGTATGTTTTTAAGTGTGCATATAGTCTTTTATCTAATTCAATAGCAATTAAAATATTTGTTTTTTGTACAAGAATTTCAGTAATTGCTCCTCACCCTGGACCAATTTCTAATATAAGATCATCTTTATTAATATTCGCCACATCTACAATTTTATTTTTTATGTTATTAGACAATAAGAAGTTTTGCCCCATTTTTTTTGATGGAACAAAACTTTCTTGCTTGAGTTTATTTTTAATGAATGATTTATTCAAGTTAGCTAATTCCTAATTTAGCAAGTTTTGATTCAATTCTTGTGGCACTGTTTGAACGGCGTTTCATAATAATAACATGAATGATATACGATTGCAGAATAGTAAAGATCGCATTGAAGAATCAGTATAAACCAATCCCGGAAGCAGAAATTGCTACTACAACAGCTAAAACAATTGCAATAATGTTTTGTGTCATTCGTACACGTTTTAACTGTTGTTTATTTTTAGCACCAACAGTTGTGGCATTACGATTACGTTTTCTAGCTAATAGTTGTGGTATTTTTTGGGATAAGATTTGTACTGGAATAATAATTAATAAGAAGAAAATATAAGGTCATCCTGAAGTTGTGAAATTTGAAAAAATTTCAGAAATAGGTGTTGCTGATAAGTCTCAAATATAAAAAATACTAATAAACTTAAGTGGTCTTAAAATTGTAACAACACGATAAATAATTAAAAAGATCGGCAATGTAATAATCATACTTTCAAAAGGAGCTAAAGGTTTAACGTTATGTTTTTGATATAACTCTTTTGTTTCCATTTGTTTTTTTTGACGAGACTGCATATCTTTTGCGTCTTTATATTTTGCATTAATTTCTGCAATTTTTCCTTGAATTTCAGAAATTCTTTCTGTTTGGATAGTTGCACGTGCTGAGATCAACATTGTAATCACACGAATAATTAATAAAATGATAATTAATCCTAAAATAGCATTTAAACCTGCTGGTCAATCTCTCGTTGCATACATAAAATGTAATAAAATTTGTGCAATTGGCCAAACAAATAAAGCATAGAAAGGACCATAATCAAATGAAAAATCAGACATTGGATATCAAGGACCTGTTCCAGAATAAATTAAATCAAAACGAATGTCATTAGCACCAAGAATAGGATCAACATGAAATCCTAATTCAAAACCATTACCAACAATAGTAGAATTAATTGTTCAGTGGTTTGCCATATTTTGGAAACAACCATATAAGCCAATACCTAAGAAAAATGCATAAACGATGATTTTAAAAACTTTTAATAAAACATTAAAAATTTTTTTCTTACGTTCTTTTTTTATCGAATTTGCATTCGATGCTCCAGCAAAATGTGAAACAGAAATCCGCATTCTATTTGTTAAATTTTGTTTATCAACAGATGACATTTATACCTCCTTTTCAATTCAAAAATTACTTGCTAATTTTATCTTTTTTCTTTGTTAATAATTCGTTGAAGAATTATTTTAAGATTATTACAATTTAAAACAAAAGAACCATCAATAAACGATGGTTTCACTAAGACTACAATATCATAACTTTTAATGGGAATATCAGCAGCAATAAACCATGCTTTAATCAGGCGTCGAATTCGATTGCGTTGTGTTGCTAATTTAAACTTTTTTTTAGAAATGCTTATAGCTAAGCGAACATTTTTTAGATTGGTTTTACGAAAATAAACTACGATTTGATTAGAATGAATTTTTTGTTGTTTCTTAATTGTATCAAGTATATCTTCATTTTTTTTTAGACTAATAAAATTAGCCATAAAAATTATTTTTCACTAGAAACTGTTAGACTATGACGCCCTTTAAGTCTTCTTCTAGCTAAAACATTACGCCCATTTTTTGTTTTCATGCGTGCACGAAAACCATGCACTTTTGCTCTTTTACGATTATTAGGTTGAAACGTTCTTTTCATAAATCCACCGCCCTCTTACTTTTTTGAAAACATAATATGGATTATTATAACATTTTAGTTATTTTTTATTTAATATATTTTTTTAAAAAAGTCAATGATATCTTTTTAAAAATAAACATATATAATATGATAATAGGACAAAGATTATTTATAAAAAATAGAGGTTACTA", "length": 751719, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_000006625.1|AF222894.1", "orig_description": "HMT-799 Ureaplasma parvum ATCC 700970" } ], "run": { "start": "2026-01-25 19:14:56", "end": "2026-01-25 19:22:40", "duration": "7.75 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }