{ "genome": { "genus": "Mycoplasmoides", "species": "genitalium", "strain": "G-37", "taxon": "Mycoplasmoides genitalium G-37", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 580076, "gc": 0.31689123494162835, "n_ratio": 0.0, "n50": 580076, "n90": 580076, "coding_ratio": 0.7886794144215585 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 686, "stop": 1828, "strand": "+", "frame": 2, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360", "KEGG:K02338", "RefSeq:WP_009885562.1", "SO:0001217", "UniParc:UPI0000557BEB", "UniRef:UniRef100_P47247", "UniRef:UniRef50_P47247", "UniRef:UniRef90_P47247" ], "nt": "ATGAAAATATTAATTAATAAAAGTGAATTGAATAAAATTTTGAAAAAAATGAATAACGTTATTATTTCCAATAACAAAATAAAACCACATCATTCATATTTTTTAATAGAGGCAAAAGAAAAAGAAATAAACTTTTATGCTAACAATGAATACTTTTCTGTCAAATGTAATTTAAATAAAAATATTGATATTCTTGAACAAGGCTCCTTAATTGTTAAAGGAAAAATTTTTAACGATCTTATTAATGGCATAAAAGAAGAGATTATTACTATTCAAGAAAAAGATCAAACACTTTTGGTTAAAACAAAAAAAACAAGTATTAATTTAAACACAATTAATGTGAATGAATTTCCAAGAATAAGGTTTAATGAAAAAAACGATTTAAGTGAATTTAATCAATTCAAAATAAATTATTCACTTTTAGTAAAAGGCATTAAAAAAATTTTTCACTCAGTTTCAAATAATCGTGAAATATCTTCTAAATTTAATGGAGTAAATTTCAATGGATCCAATGGAAAAGAAATATTTTTAGAAGCTTCTGACACTTATAAACTATCTGTTTTTGAGATAAAGCAAGAAACAGAACCATTTGATTTCATTTTGGAGAGTAATTTACTTAGTTTCATTAATTCTTTTAATCCTGAAGAAGATAAATCTATTGTTTTTTATTACAGAAAAGATAATAAAGATAGCTTTAGTACAGAAATGTTGATTTCAATGGATAACTTTATGATTAGTTACACATCGGTTAATGAAAAATTTCCAGAGGTAAACTACTTTTTTGAATTTGAACCTGAAACTAAAATAGTTGTTCAAAAAAATGAATTAAAAGATGCACTTCAAAGAATTCAAACTTTGGCTCAAAATGAAAGAACTTTTTTATGCGATATGCAAATTAACAGTTCTGAATTAAAAATAAGAGCTATTGTTAATAATATCGGAAATTCTCTTGAGGAAATTTCTTGTCTTAAATTTGAAGGTTATAAACTTAATATTTCTTTTAACCCAAGTTCTCTATTAGATCACATAGAGTCTTTTGAATCAAATGAAATAAATTTTGATTTCCAAGGAAATAGTAAGTATTTTTTGATAACCTCTAAAAGTGAACCTGAACTTAAGCAAATATTGGTTCCTTCAAGATAA", "aa": "MKILINKSELNKILKKMNNVIISNNKIKPHHSYFLIEAKEKEINFYANNEYFSVKCNLNKNIDILEQGSLIVKGKIFNDLINGIKEEIITIQEKDQTLLVKTKKTSINLNTINVNEFPRIRFNEKNDLSEFNQFKINYSLLVKGIKKIFHSVSNNREISSKFNGVNFNGSNGKEIFLEASDTYKLSVFEIKQETEPFDFILESNLLSFINSFNPEEDKSIVFYYRKDNKDSFSTEMLISMDNFMISYTSVNEKFPEVNYFFEFEPETKIVVQKNELKDALQRIQTLAQNERTFLCDMQINSSELKIRAIVNNIGNSLEEISCLKFEGYKLNISFNPSSLLDHIESFESNEINFDFQGNSKYFLITSKSEPELKQILVPSR", "aa_hexdigest": "dbe7697324ca34cfcc18c9862e72c06e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557BEB", "ncbi_nrp_id": "WP_009885562.1", "uniref100_id": "UniRef100_P47247", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557BEB", "RefSeq:WP_009885562.1", "UniRef:UniRef100_P47247" ] }, "ips": { "uniref100_id": "UniRef100_P47247", "uniref90_id": "UniRef90_P47247" }, "psc": { "uniref90_id": "UniRef90_P47247", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47247", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_P47247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47247" ], "product": "Beta sliding clamp" }, "genes": [ "dnaN" ], "id": "KKNAAMEMLJ_43", "locus": "GCA000027325_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1828, "stop": 2073, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00226.36" ], "nt": "ATGAATCTTTACGATCTTTTAGAACTACCAACTACAGCATCAATAAAAGAAATAAAAATTGCTTATAAAAGATTAGCAAAGCGTTATCACCCTGATGTAAATAAATTAGGTTCGCAAACTTTTGTTGAAATTAATAATGCTTATTCAATATTAAGTGATCCTAACCAAAAGGAAAAATATGATTCAATGCTGAAAGTTAATGATTTTCAAAATCGCATCAAAAATTTAGATATTAGTGTTAGATGA", "aa": "MNLYDLLELPTTASIKEIKIAYKRLAKRYHPDVNKLGSQTFVEINNAYSILSDPNQKEKYDSMLKVNDFQNRIKNLDISVR", "aa_hexdigest": "bd68886341465ed340ba720a06a2fb0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00226.36", "name": "DnaJ", "length": 63, "aa_cov": 0.7407407407407407, "hmm_cov": 1.0, "evalue": 1.7167254092710034e-22, "score": 74.42524719238281, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 9475.772099999996, "isoelectric_point": 9.221831703186034 }, "id": "KKNAAMEMLJ_44", "locus": "GCA000027325_00002" }, { "type": "cds", "sequence": "contig_1", "start": 2605, "stop": 2760, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTAGTTAATAAAGTAAACAAAAGACTGCGTATTTTTTCAAGCTTTTTTGAGAACGATAAATCTAAATTATGGTTCCTTGTTCCAAACGATAAACAAAGTAATCCTAATAAGGGCGTTTTTAACTATAAAACTCAGCACTTTATTGATTAA", "aa": "MKVVNKVNKRLRIFSSFFENDKSKLWFLVPNDKQSNPNKGVFNYKTQHFID", "aa_hexdigest": "7ccff5a9fba33d568e382fcbb1e0eb7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6148.016900000002, "isoelectric_point": 10.092283821105958 }, "id": "KKNAAMEMLJ_45", "locus": "GCA000027325_00003" }, { "type": "cds", "sequence": "contig_1", "start": 2845, "stop": 2979, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "nt": "ATGGAAGAAAATAACAAAGCAAATATCTATGACTCTAGTAGCATTAAGGTCCTTGAAGGACTTGAGGCTGTTAGAAAACGCCCTGGAATGTACATTGGTTCTACTGGCGAAGAAGGTTTGCATCACATGATCTGA", "aa": "MEENNKANIYDSSSIKVLEGLEAVRKRPGMYIGSTGEEGLHHMI", "aa_hexdigest": "838391e1dd8186643f04d3bd2b89c52e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920FT50", "query_cov": 0.9090909090909091, "subject_cov": 0.8, "identity": 0.7, "score": 58.5, "evalue": 1.34e-10, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A920FT50" }, "pscc": { "uniref50_id": "UniRef50_A0A920FT50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "KKNAAMEMLJ_46", "locus": "GCA000027325_00004" }, { "type": "cds", "sequence": "contig_1", "start": 3010, "stop": 3255, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "nt": "ATGGGAGGTTTTGCCAGTTTTGTTAAGCTTACCCTTGAAGATAATTTTGTTACCCGTGTAGAGGATGATGGAAGAGGGATACCTGTTGATATCCATCCTAAGACTAATCGTTCTACAGTTGAAACAGTTTTTACAGTTCTACACGCTGGCGGTAAATTTGATAACGATAGCTATAAAGTGTCAGGTGGTTTACACGGTGTTGGTGCATCAGTTGTTAATGCGCTTAGTTCTTCTTTTAAAGTTTGA", "aa": "MGGFASFVKLTLEDNFVTRVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKV", "aa_hexdigest": "930d6e6912b2909e276c273e3dce4c94", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021B43CD8", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.568, "score": 93.2, "evalue": 2.53e-23, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A380EBM1" }, "pscc": { "uniref50_id": "UniRef50_A0A380EBM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "KKNAAMEMLJ_47", "locus": "GCA000027325_00005" }, { "type": "cds", "sequence": "contig_1", "start": 3319, "stop": 3513, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCCAAGAAGGTAACTCTGAAAAAGAGCATGGAACAATTGTTGAGTTTGTTCCTGATTTCTCTGTAATGGAAAAGAGTGATTACAAACAAACTGTAATTGTAAGCAGACTCCAGCAATTAGCTTTTTTAAACAAGGGAATAAGAATTGACTTTGTTGATAATCGTAAACAAAACCCACAGTCTTTTTCTTGA", "aa": "MVQEGNSEKEHGTIVEFVPDFSVMEKSDYKQTVIVSRLQQLAFLNKGIRIDFVDNRKQNPQSFS", "aa_hexdigest": "5dc3e325e8cc02961f18c22b3972012f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7404.266600000002, "isoelectric_point": 5.678067588806152 }, "id": "KKNAAMEMLJ_48", "locus": "GCA000027325_00006" }, { "type": "cds", "sequence": "contig_1", "start": 3529, "stop": 4557, "strand": "+", "frame": 1, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P22447", "UniRef:UniRef90_P22447" ], "nt": "TTGGTTGAATATATCCACCACCTAAACAACGAAAAAGAACCACTTTTTAATGAAGTTATTGCTGATGAAAAAACTGAAACTGTAAAAGCTGTTAATCGTGATGAAAACTACACAGTAAAGGTTGAAGTTGCTTTTCAATATAACAAAACATACAACCAATCAATTTTCAGTTTTTGTAACAACATTAATACTACAGAAGGTGGAACCCATGTGGAAGGTTTTCGTAATGCACTTGTTAAGATCATTAATCGCTTTGCTGTTGAAAATAAATTCCTAAAAGATAGTGATGAAAAGATTAACCGTGATGATGTTTGTGAAGGATTAACTGCTATTATTTCCATTAAACACCCAAACCCACAATATGAAGGACAAACTAAAAAGAAGTTAGGTAATACTGAGGTAAGACCTTTAGTTAATAGTGTTGTTAGTGAAATCTTTGAACGCTTCATGTTAGAAAACCCACAAGAAGCAAACGCTATCATCAGAAAAACACTTTTAGCTCAAGAAGCGAGAAGAAGAAGTCAAGAGGCTAGGGAGTTAACTCGTCGTAAATCACCTTTTGATAGTGGTTCATTACCAGGTAAATTAGCTGATTGTACAACCAGAGATCCTTCGATTAGTGAACTTTACATTGTTGAGGGTGATAGTGCTGGTGGCACTGCTAAAACAGGAAGAGATCGTTATTTTCAAGCTATCTTACCCTTAAGAGGAAAGATTTTAAACGTTGAAAAATCTAACTTTGAACAAATCTTTAATAATGCAGAAATTTCTGCATTAGTGATGGCAATAGGCTGTGGGATTAAACCTGATTTTGAACTTGAAAAACTTAGATATAGCAAGATTGTGATCATGACAGATGCTGATGTTGATGGTGCACACATAAGAACACTTCTCTTAACTTTCTTTTTTCGCTTTATGTATCCTTTGGTTGAACAAGGCAATATTTTTATTGCTCAACCCCCACTTTATAAAGTGTCATATTCCCATAAGGATTTATACATGCACACTGATGTTCAACTTGAACAGTGA", "aa": "MVEYIHHLNNEKEPLFNEVIADEKTETVKAVNRDENYTVKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKDSDEKINRDDVCEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSVVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSNFEQIFNNAEISALVMAIGCGIKPDFELEKLRYSKIVIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIFIAQPPLYKVSYSHKDLYMHTDVQLEQ", "aa_hexdigest": "a401d9d9670848dc5b513361b388c93d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5R7J3", "query_cov": 1.0, "subject_cov": 0.8260869565217391, "identity": 0.5379999999999999, "score": 366.0, "evalue": 3.5e-122, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_K2BJ35" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 3229, "stop": 4797, "observations": { "mutation": [ 3253, 3511, 4555, 4633 ], "stop": [ 3253, 3511, 4555, 4633 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P22447", "query_cov": 1.0, "subject_cov": 0.5261538461538462, "identity": 0.997, "score": 670.0, "evalue": 2.79e-238, "gene_start": 1, "gene_end": 342, "reference_start": 229, "reference_end": 570, "reference_sequence": "MEENNKANIYDSSSIKVLEGLEAVRKRPGMYIGSTGEEGLHHMIWEIVDNSIDEAMGGFASFVKLTLEDNFVTRVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVFRQNKKYFLSFSDGGKVIGDLVQEGNSEKEHGTIVEFVPDFSVMEKSDYKQTVIVSRLQQLAFLNKGIRIDFVDNRKQNPQSFSWKYDGGLVEYIHHLNNEKEPLFNEVIADEKTETVKAVNRDENYTVKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKDSDEKINRDDVCEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSVVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSNFEQIFNNAEISALVMAIGCGIKPDFELEKLRYSKIVIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIFIAQPPLYKVSYSHKDLYMHTDVQLEQWKSQNPNVKFGLQRYKGLGEMDALQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEKNARSVKNIDI" }, "paralog": false, "identity": 0.9923371647509579, "score": 1017.0, "evalue": 0.0, "description": "Internal stop codon at 3253, 3511, 4555, 4633. Nonsense mutation around 3253, 3511, 4555, 4633.", "psc": { "uniref90_id": "UniRef90_P22447", "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P22447", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P22447", "EC:5.6.2.2", "UniRef:UniRef50_P22447", "COG:COG0187", "COG:L", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P22447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P22447" ], "product": "DNA gyrase subunit B" } }, "id": "KKNAAMEMLJ_49", "locus": "GCA000027325_00007" }, { "type": "cds", "sequence": "contig_1", "start": 4645, "stop": 4797, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCCTAAGGTTAGAACATTGTTAAAAGTTACTGTTGAAGATGCTTCTATTGCTGATAAAGCTTTTTCACTGTTGATGGGTGATGAAGTTCCCCCAAGAAGAGAATTTATTGAAAAAAATGCTCGTAGTGTTAAAAACATTGATATTTAA", "aa": "MDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEKNARSVKNIDI", "aa_hexdigest": "005320cf15fac2ee99e015a052764196", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A926IWG4", "query_cov": 1.0, "subject_cov": 0.8333333333333334, "identity": 0.68, "score": 68.2, "evalue": 3.33e-14, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A926IWG4" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5657.521399999999, "isoelectric_point": 6.141760063171386 }, "id": "KKNAAMEMLJ_50", "locus": "GCA000027325_00008" }, { "type": "cds", "sequence": "contig_1", "start": 4812, "stop": 7322, "strand": "+", "frame": 3, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335", "KEGG:K02469", "RefSeq:WP_009885559.1", "SO:0001217", "UniParc:UPI000012BE8C", "UniRef:UniRef100_P47250", "UniRef:UniRef50_P47719", "UniRef:UniRef90_P47250" ], "nt": "ATGGCAAAGCAACAAGATCAAGTAGATAAGATTCGTGAAAACTTAGACAATTCAACTGTCAAAAGTATTTCATTAGCAAATGAACTTGAGCGTTCATTCATGGAATATGCTATGTCAGTTATTGTTGCTCGTGCTTTACCTGATGCTAGAGATGGACTTAAACCAGTTCATCGTCGTGTTCTTTATGGTGCTTATATTGGTGGCATGCACCATGATCGTCCTTTTAAAAAGTCTGCGAGGATTGTTGGTGATGTAATGAGTAAATTCCACCCTCATGGTGATATGGCAATATATGACACCATGTCAAGAATGGCTCAAGACTTTTCATTAAGATACCTTTTAATTGATGGTCATGGTAATTTTGGTTCTATAGATGGTGATAGACCTGCTGCACAACGTTATACAGAAGCAAGATTATCTAAACTTGCAGCAGAACTTTTAAAAGATATTGATAAAGATACAGTTGACTTTATTGCTAATTATGATGGTGAGGAAAAAGAACCAACTGTTCTACCAGCAGCTTTCCCTAACTTACTTGCAAATGGTTCTAGTGGGATTGCAGTTGGAATGTCAACATCTATTCCTTCCCATAATCTCTCTGAATTAATTGCGGGTTTAATCATGTTAATTGATAATCCTCAATGCACTTTTCAAGAATTATTAACTGTAATTAAAGGACCTGATTTTCCAACAGGAGCTAACATTATCTACACAAAAGGAATTGAAAGCTACTTTGAAACAGGTAAAGGCAATGTAGTAATTCGTTCTAAAGTTGAGATAGAACAATTGCAAACAAGAAGTGCATTAGTTGTAACTGAAATTCCTTACATGGTTAACAAAACTACCTTAATTGAAAAGATTGTAGAACTTGTTAAAGCTGAAGAGATTTCAGGAATTGCTGATATCCGTGATGAATCCTCTCGAGAAGGAATAAGGTTAGTGATTGAAGTAAAACGCGACACTGTACCTGAAGTTTTATTAAATCAACTTTTTAAATCAACAAGATTACAAGTACGCTTCCCTGTTAATATGCTTGCTTTAGTTAAAGGAGCTCCTGTACTTCTCAACATGAAACAAGCTTTGGAAGTATATCTTGATCATCAAATTGATGTTCTTGTTAGAAAAACAAAGTTTGTGCTTAATAAACAACAAGAACGTTATCACATTTTAAGCGGACTTTTAATTGCTGCTTTAAATATTGATGAGGTTGTTGCAATTATTAAAAAATCAGCAAATAACCAGGAAGCAATTAATACATTAAATACAAAGTTTAAGCTTGATGAAATTCAAGCTAAAGCAGTTCTTGACATGCGTTTAAGGAGCTTAAGCGTACTTGAAGTTAACAAACTTCAAACTGAACAAAAAGAGTTAAAAGATTCAATTGAATTTTGTAAGAAAGTGTTAGCTGATCAAAAATTACAGCTAAAAATAATCAAAGAGGAATTGCAAAAAATCAATGATCAGTTTGGTGATGAAAGAAGAAGTGAAATTCTCTATGATATCTCTGAGGAAATTGATGATGAATCATTGATAAAAGTTGAGAATGTAGTGATAACTATGTCTACAAATGGTTATCTAAAAAGGATTGGAGTTGATGCTTATAATCTTCAACATCGTGGTGGAGTTGGGGTTAAAGGGCTAACTACTTATGTTGATGATAGTATTAGTCAATTATTGGTCTGTTCAACTCACTCTGACTTATTATTTTTTACTGATAAGGGTAAGGTTTATAGAATTAGAGCTCATCAAATTCCCTATGGTTTTAGAACAAATAAAGGTATTCCCGCTGTTAACTTAATCAAAATTGAAAAGGATGAAAGAATTTGTTCATTGTTATCTGTTAATAACTATGATGATGGTTATTTCTTTTTCTGTACTAAAAATGGAATTGTTAAAAGAACGAGCTTGAATGAATTCATCAACATCTTAAGTAATGGTAAGCGGGCTATATCTTTTGATGATAATGACACTTTGTATTCAGTAATTAAAACCCACGGAAATGATGAGATTTTTATTGGTTCTACCAATGGATTTGTTGTTCGCTTCCATGAAAATCAACTCAGAGTTCTTTCAAGAACAGCAAGAGGTGTATTTGGTATCAGTTTAAATAAAGGAGAATTTGTTAATGGACTATCAACTTCAAGCAACGGTAGCTTACTTTTATCAGTCGGTCAAAATGGAATAGGTAAATTAACGAGCATAGATAAATATAGACTCACAAAACGTAATGCTAAGGGAGTTAAAACTCTAAGGGTTACTGATAGAACAGGCCCTGTTGTTACAACAACCACTGTTTTTGGTAATGAGGATCTTTTAATGATTTCCTCTGCTGGTAAAATTGTGCGTACCAGTTTACAAGAACTTTCAGAACAAGGTAAAAACACTTCTGGTGTTAAGTTAATTAGATTAAAAGATAATGAACGTTTAGAAAGAGTAACTATCTTTAAAGAAGAGTTAGAAGACAAAGAAATGCAACTAGAAGATGTTGGATCCAAACAAATTACGCAATAA", "aa": "MAKQQDQVDKIRENLDNSTVKSISLANELERSFMEYAMSVIVARALPDARDGLKPVHRRVLYGAYIGGMHHDRPFKKSARIVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGNFGSIDGDRPAAQRYTEARLSKLAAELLKDIDKDTVDFIANYDGEEKEPTVLPAAFPNLLANGSSGIAVGMSTSIPSHNLSELIAGLIMLIDNPQCTFQELLTVIKGPDFPTGANIIYTKGIESYFETGKGNVVIRSKVEIEQLQTRSALVVTEIPYMVNKTTLIEKIVELVKAEEISGIADIRDESSREGIRLVIEVKRDTVPEVLLNQLFKSTRLQVRFPVNMLALVKGAPVLLNMKQALEVYLDHQIDVLVRKTKFVLNKQQERYHILSGLLIAALNIDEVVAIIKKSANNQEAINTLNTKFKLDEIQAKAVLDMRLRSLSVLEVNKLQTEQKELKDSIEFCKKVLADQKLQLKIIKEELQKINDQFGDERRSEILYDISEEIDDESLIKVENVVITMSTNGYLKRIGVDAYNLQHRGGVGVKGLTTYVDDSISQLLVCSTHSDLLFFTDKGKVYRIRAHQIPYGFRTNKGIPAVNLIKIEKDERICSLLSVNNYDDGYFFFCTKNGIVKRTSLNEFINILSNGKRAISFDDNDTLYSVIKTHGNDEIFIGSTNGFVVRFHENQLRVLSRTARGVFGISLNKGEFVNGLSTSSNGSLLLSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTDRTGPVVTTTTVFGNEDLLMISSAGKIVRTSLQELSEQGKNTSGVKLIRLKDNERLERVTIFKEELEDKEMQLEDVGSKQITQ", "aa_hexdigest": "f92bc4b44c4f2d5989364788f6845074", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012BE8C", "ncbi_nrp_id": "WP_009885559.1", "uniref100_id": "UniRef100_P47250", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012BE8C", "RefSeq:WP_009885559.1", "UniRef:UniRef100_P47250" ] }, "ips": { "uniref100_id": "UniRef100_P47250", "uniref90_id": "UniRef90_P47250" }, "psc": { "uniref90_id": "UniRef90_P47250", "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47719", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47719", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47719" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "KKNAAMEMLJ_51", "locus": "GCA000027325_00009" }, { "type": "cds", "sequence": "contig_1", "start": 7294, "stop": 7857, "strand": "+", "frame": 1, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_P47251", "UniRef:UniRef90_P47251" ], "nt": "ATGTTGGATCCAAACAAATTACGCAATAACTATGATTTCTTTAAAAAGAAACTGTTAGAAAGAAATGTAAATGAGCAATTATTAAATCAGTTTATTCAAACTGATAAACTAATGCGCAAAAACTTGCAACAACTTGAACTTGCTAACCAAAAACAAAGCTTGTTGGCAAAACAAGTTGCTAAGCAAAAAGATAATAAAAAGCTATTAGCTGAATCAAAAGAACTTAAGCAGAAGATTGAAAACTTAAATAATGCTTATAAAGATTCACAAAACATTAGTCAAGATTTACTTCTAAATTTTCCTAATATTGCTCATGAATCAGTTCCTGTTGGTAAAAATGAATCAGCAAACTTAGAACTTCTTAAAGAAGGGAGAAAACCAGTTTTTGATTTCAAACCTTTACCACATCGAGAGTTATGTGAAAAGTTAAATTTAGTTGCTTTTGATAAAGCTACTAAGATTAGTGGAACTAGGTTTGTTGCATATACAGATAAAGCAGCTAAACTACTTAGAGCGATAACTAATCTAATGATTGACCTTAATAAAAGCAAGTATCAAGAATGA", "aa": "MLDPNKLRNNYDFFKKKLLERNVNEQLLNQFIQTDKLMRKNLQQLELANQKQSLLAKQVAKQKDNKKLLAESKELKQKIENLNNAYKDSQNISQDLLLNFPNIAHESVPVGKNESANLELLKEGRKPVFDFKPLPHRELCEKLNLVAFDKATKISGTRFVAYTDKAAKLLRAITNLMIDLNKSKYQE", "aa_hexdigest": "d85b489586b61a5325ce0e37450e9200", "start_type": "ATG", "rbs_motif": null, "genes": [ "serS" ], "truncated": "3-prime", "pseudogene": { "start": 7294, "stop": 8160, "observations": { "mutation": [ 7855 ], "stop": [ 7855 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47251", "query_cov": 1.0, "subject_cov": 0.44844124700239807, "identity": 1.0, "score": 349.0, "evalue": 6.3e-118, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MLDPNKLRNNYDFFKKKLLERNVNEQLLNQFIQTDKLMRKNLQQLELANQKQSLLAKQVAKQKDNKKLLAESKELKQKIENLNNAYKDSQNISQDLLLNFPNIAHESVPVGKNESANLELLKEGRKPVFDFKPLPHRELCEKLNLVAFDKATKISGTRFVAYTDKAAKLLRAITNLMIDLNKSKYQEWNLPVVINELSLRSTGQLPKFKDDVFKLENTRYYLSPTLEVQLINLHANEIFNEEDLPKYYTATGINFRQEAGSAGKQTKGTIRLHQFQKTELVKFCKPENAINELEAMVRDAEQILKALKLPFRRLLLCTGDMGFSAEKTYDLEVWMAASNEYREVSSCSSCGDFQARRAMIRYKDINNGKNSYVATLNGTALSIDRIFAAILENFQTKDGKILIPQALKKYLDFDTIK" }, "paralog": false, "identity": 0.9965277777777778, "score": 565.0, "evalue": 1.23e-205, "description": "Internal stop codon at 7855. Nonsense mutation around 7855.", "psc": { "uniref90_id": "UniRef90_P47251", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_P47251", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47251", "EC:6.1.1.11", "UniRef:UniRef50_P47251", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ] }, "pscc": { "uniref50_id": "UniRef50_P47251", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47251" ], "product": "Serine--tRNA ligase" } }, "id": "KKNAAMEMLJ_52", "locus": "GCA000027325_00010" }, { "type": "cds", "sequence": "contig_1", "start": 8107, "stop": 8295, "strand": "+", "frame": 1, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0032988E16" ], "nt": "TTGCATCAGTTTCAAAAAACTGAGTTAGTTAAGTTTTGTAAACCTGAAAATGCTATCAATGAATTGGAAGCAATGGTTAGAGATGCTGAACAAATCTTAAAGGCACTTAAGTTACCTTTTAGAAGGTTATTGTTATGTACTGGTGATATGGGCTTTAGTGCTGAAAAAACATATGATCTTGAAGTTTGA", "aa": "MHQFQKTELVKFCKPENAINELEAMVRDAEQILKALKLPFRRLLLCTGDMGFSAEKTYDLEV", "aa_hexdigest": "e28a48f94f7fd7ff0c03f1b7e954a7db", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002F2627E6", "query_cov": 1.0, "subject_cov": 0.8857142857142857, "identity": 0.5479999999999999, "score": 73.6, "evalue": 5.18e-16, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_UPI0032988E16", "kegg_orthology_id": "K01875" }, "pscc": { "uniref50_id": "UniRef50_UPI0032988E16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0032988E16" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "KKNAAMEMLJ_53", "locus": "GCA000027325_00011" }, { "type": "cds", "sequence": "contig_1", "start": 8296, "stop": 8547, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGGCAGCTAGCAATGAATATCGTGAAGTTTCTTCTTGTTCATCTTGTGGTGATTTTCAAGCAAGAAGAGCTATGATTCGTTACAAAGATATTAACAACGGTAAAAACAGTTATGTTGCTACTTTAAATGGAACAGCATTATCTATTGATAGAATTTTTGCTGCAATTCTAGAAAATTTTCAAACAAAAGATGGCAAAATTCTTATCCCACAAGCATTAAAAAAATACCTTGATTTTGACACAATCAAGTAA", "aa": "MAASNEYREVSSCSSCGDFQARRAMIRYKDINNGKNSYVATLNGTALSIDRIFAAILENFQTKDGKILIPQALKKYLDFDTIK", "aa_hexdigest": "dcc1f77d2836fa16e7d8406aa61056ff", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00345BB37E", "query_cov": 0.891566265060241, "subject_cov": 0.8222222222222222, "identity": 0.5539999999999999, "score": 89.4, "evalue": 1.17e-21, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_A0A3B8WPJ3", "kegg_orthology_id": "K01875" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 57, "aa_cov": 0.6867469879518072, "hmm_cov": 0.9649122807017544, "evalue": 2.056382817150307e-09, "score": 32.797603607177734, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 9314.53189999999, "isoelectric_point": 9.001543235778808 }, "id": "KKNAAMEMLJ_54", "locus": "GCA000027325_00012" }, { "type": "cds", "sequence": "contig_1", "start": 8551, "stop": 9183, "strand": "+", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "RefSeq:WP_009885557.1", "SO:0001217", "UniParc:UPI0000032EAD", "UniRef:UniRef100_P47252", "UniRef:UniRef50_P47252", "UniRef:UniRef90_P47252" ], "nt": "ATGAATAAAGGTGTTTTTGTTGTTATTGAAGGAGTTGATGGAGCGGGCAAAACTGCTTTAATTGAAGGTTTTAAAAAACTTTATCCAACTAAGTTTTTGAACTATCAACTTACTTATACTAGAGAACCTGGTGGTACTTTGTTAGCTGAAAAAATTCGTCAACTTCTTTTAAATGAAACAATGGAACCTCTAACTGAAGCTTATTTGTTTGCCGCAGCTAGAACTGAACATATCAGTAAGCTAATTAAACCAGCAATTGAAAAAGAACAACTAGTTATTTCAGATAGATTTGTTTTCTCTAGTTTTGCATACCAAGGATTAAGCAAAAAAATAGGCATTGATACAGTAAAACAGATTAATCATCATGCGTTAAGAAATATGATGCCAAACTTTACCTTTATTTTGGATTGCAATTTTAAAGAAGCATTACAAAGGATGCAAAAGCGTGGTAATGATAATCTTCTTGATGAATTTATTAAAGGAAAGAATGATTTTGATACAGTTCGTTCTTATTATTTAAGCTTAGTTGATAAAAAAAACTGTTTCTTGATTAATGGTGATAATAAACAAGAACACCTAGAGAAATTTATTGAATTGTTAACAAGATGCTTACAACAACCCACGCATTACTAA", "aa": "MNKGVFVVIEGVDGAGKTALIEGFKKLYPTKFLNYQLTYTREPGGTLLAEKIRQLLLNETMEPLTEAYLFAAARTEHISKLIKPAIEKEQLVISDRFVFSSFAYQGLSKKIGIDTVKQINHHALRNMMPNFTFILDCNFKEALQRMQKRGNDNLLDEFIKGKNDFDTVRSYYLSLVDKKNCFLINGDNKQEHLEKFIELLTRCLQQPTHY", "aa_hexdigest": "b0628416374409bfb1dc17ac3d82a808", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000032EAD", "ncbi_nrp_id": "WP_009885557.1", "uniref100_id": "UniRef100_P47252", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000032EAD", "RefSeq:WP_009885557.1", "UniRef:UniRef100_P47252" ] }, "ips": { "uniref100_id": "UniRef100_P47252", "uniref90_id": "UniRef90_P47252" }, "psc": { "uniref90_id": "UniRef90_P47252", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_P47252", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_P47252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47252" ], "product": "Thymidylate kinase" }, "genes": [ "tmk" ], "id": "KKNAAMEMLJ_55", "locus": "GCA000027325_00013" }, { "type": "cds", "sequence": "contig_1", "start": 9156, "stop": 9323, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTACAACAACCCACGCATTACTAATCATTCAAAGAAAAGGTAGTTTCTTAAAACCTTTTCTTGATAATTATCTTACTAGTATTGTTTGTGAAAACAAAAATGGTTGCAAAAAGTGTATAAACTGTTTGGAAATTCTCAATAATAAATACAACAGCTTATATTGA", "aa": "MLTTTHALLIIQRKGSFLKPFLDNYLTSIVCENKNGCKKCINCLEILNNKYNSLY", "aa_hexdigest": "eaef039d4c156b9a8950d9e6f9012a48", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6339.452299999999, "isoelectric_point": 9.046606636047361 }, "id": "KKNAAMEMLJ_56", "locus": "GCA000027325_00014" }, { "type": "cds", "sequence": "contig_1", "start": 9468, "stop": 9755, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGACTAGTTGAAGATAGTCCGATAAATAGTTATGGTATTTTTACAACTAAAAATGAAAGTTTAATTCTTTCCACTTTTTTAAGTAGAGTACAGAAAGTAGTTTTAAAAAAAGCTAGTAAAGTTCCTTTTAAAGTAAGCAAAAATGATCAAGAAATTATTACAAGTTTTTTTACTGTAGATGAACAAATTGAAGCAATTGAAAATGGAAGTTTTAACCGTTTCAAAATTATCTTAGATGCATGTTTAAACAAAAAAACAGGTACAGAACAAATTTATCATGCTTGA", "aa": "MRLVEDSPINSYGIFTTKNESLILSTFLSRVQKVVLKKASKVPFKVSKNDQEIITSFFTVDEQIEAIENGSFNRFKIILDACLNKKTGTEQIYHA", "aa_hexdigest": "1ad9da980b4fbfcd8b0643c6465d8fab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10816.33169999999, "isoelectric_point": 8.75959339141846 }, "id": "KKNAAMEMLJ_57", "locus": "GCA000027325_00015" }, { "type": "cds", "sequence": "contig_1", "start": 9923, "stop": 11059, "strand": "+", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "EC:3.6.-.-", "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47254", "UniRef:UniRef90_P47254" ], "nt": "ATGAAAAGCGAAATTAATATTTTTGCACTAGCAACTGCACCTTTTAATAGTGCATTACATATTATTAGGTTTTCTGGTCCTGATGTTTATGAGATTTTAAACAAGATAACTAATAAAAAAATAACAAGAAAAGGGATGCAAATTCAACGCACATGGATAGTTGATGAAAACAATAAGCGAATTGATGATGTGCTATTATTTAAATTTGTCTCTCCAAATTCTTATACAGGAGAAGATTTAATTGAAATTTCTTGTCATGGTAACATGTTGATCGTTAATGAAATTTGCGCACTTCTTTTAAAAAAAGGAGGTGTTTATGCCAAACCTGGTGAATTTACCCAAAGGAGTTTTTTAAATGGAAAAATGAGTTTACAACAAGCTAGTGCTGTAAATAAATTGATTTTATCTCCTAACTTATTAGTTAAAGATATAGTCTTAAATAATTTAGCGGGTGAAATGGATCAACAATTAGAACAAATAGCTCAACAAGTTAATCAATTAGTAATGCAAATGGAAGTAAACATTGATTATCCAGAATATCTTGATGAACAAGTAGAACTATCAACTTTAAATAATAAAGTTAAATTGATTATTGAAAAGCTTAAAAGAATTATTGAAAATAGTAAACAACTCAAAAAACTTCACGATCCTTTTAAAATTGCCATTATAGGCGAAACTAATGTAGGTAAATCTTCTTTACTCAACGCTTTATTAAATCAAGATAAAGCGATAGTTTCAAATATTAAAGGTAGTACACGCGATGTTGTTGAAGGGGATTTCAATTTAAATGGTTATTTAATCAAGATCTTAGATACTGCAGGTATCCGTAAACATAAAAGTGGGCTTGAAAAAGCAGGAATTAAAAAAAGCTTTGAATCTATAAAGCAAGCTAATTTGGTTATTTATCTTTTAGATGCAACACATCCAAAGAAAGATCTTGAATTAATTAGTTTTTTTAAGAAAAATAAAAAGGATTTTTTTGTTTTCTATAACAAAAAAGATTTAATTACAAATAAGTTTGAAAATAGTATTTCTGCAAAGCAAAAAGATATTAAAGAATTAGTTGATTTATTAACTAAATATATTAACGAGTTTTATAAAAAAATAGATCAAAAAATCTATCTGATTGAAAATTGA", "aa": "MKSEINIFALATAPFNSALHIIRFSGPDVYEILNKITNKKITRKGMQIQRTWIVDENNKRIDDVLLFKFVSPNSYTGEDLIEISCHGNMLIVNEICALLLKKGGVYAKPGEFTQRSFLNGKMSLQQASAVNKLILSPNLLVKDIVLNNLAGEMDQQLEQIAQQVNQLVMQMEVNIDYPEYLDEQVELSTLNNKVKLIIEKLKRIIENSKQLKKLHDPFKIAIIGETNVGKSSLLNALLNQDKAIVSNIKGSTRDVVEGDFNLNGYLIKILDTAGIRKHKSGLEKAGIKKSFESIKQANLVIYLLDATHPKKDLELISFFKKNKKDFFVFYNKKDLITNKFENSISAKQKDIKELVDLLTKYINEFYKKIDQKIYLIEN", "aa_hexdigest": "3dccb96d80b2770b95cdfa25d59d79f0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47254", "query_cov": 1.0, "subject_cov": 0.8552036199095022, "identity": 1.0, "score": 723.0, "evalue": 9.42e-262, "valid": true, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "ec_ids": [ "3.6.-.-" ], "uniref50_id": "UniRef50_P47254", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47254" ], "product": "tRNA modification GTPase MnmE" }, "genes": [ "mnmE" ], "id": "KKNAAMEMLJ_58", "locus": "GCA000027325_00016" }, { "type": "cds", "sequence": "contig_1", "start": 11251, "stop": 11892, "strand": "+", "frame": 1, "gene": "tatD", "product": "putative metal-dependent hydrolase MG009", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.-.-", "GO:0004536", "GO:0005829", "GO:0046872", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_P47255", "UniRef:UniRef90_P47255" ], "nt": "ATGGAATACTTTGATGCACATTGTCATTTAAATTGTGAACCTTTACTGAGTGAAATTGAAAAAAGCATCGCTAATTTCAAATTAATTAATTTAAAAGCAAATGTTGTAGGTACAGATTTGGATAATTCTAAAATTGCTGTTGAATTAGCTAAAAAATATCCTGATCTTTTAAAAGCAACCATAGGTATCCATCCAAATGATGTTCATTTAGTTGATTTTAAAAAGACAAAAAAACAACTTAATGAACTATTAATAAATAACAGAAATTTCATAAGTTGTATTGGTGAATATGGTTTTGATTATCACTACACAACAGAATTTATTGAATTGCAAAACAAATTCTTTGAGATGCAATTTGAAATAGCTGAAACTAATAAATTGGTTCACATGCTTCATATTCGTGATGCTCATGAAAAAATTTATGAAATATTAACAAGATTAAAGCCAACTCAACCTGTGATTTTTCATTGTTTCAGTCAAGATATAAATATTGCTAAAAAGCTACTATCATTAAAAGATTTAAATATTGACATCTTCTTTTCTATCCCAGGGATAGTTACTTTTAAGAATGCTCAAGCATTACATGAAGCTTTAAAGATTATTCCTAGTGAATTACTTTTAAGTGAAACTGACTCACCGTGA", "aa": "MEYFDAHCHLNCEPLLSEIEKSIANFKLINLKANVVGTDLDNSKIAVELAKKYPDLLKATIGIHPNDVHLVDFKKTKKQLNELLINNRNFISCIGEYGFDYHYTTEFIELQNKFFEMQFEIAETNKLVHMLHIRDAHEKIYEILTRLKPTQPVIFHCFSQDINIAKKLLSLKDLNIDIFFSIPGIVTFKNAQALHEALKIIPSELLLSETDSP", "aa_hexdigest": "fe4aaaf19581833bce1f838a004b8d5f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47255", "query_cov": 1.0, "subject_cov": 0.8129770992366412, "identity": 1.0, "score": 427.0, "evalue": 1.68e-150, "valid": true, "gene": "TatD", "product": "Uncharacterized metal-dependent hydrolase MG009", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47255", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424", "go_ids": [ "GO:0005829", "GO:0004536", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47255" ], "product": "Uncharacterized metal-dependent hydrolase MG009" }, "genes": [ "tatD" ], "id": "KKNAAMEMLJ_59", "locus": "GCA000027325_00017" }, { "type": "cds", "sequence": "contig_1", "start": 12068, "stop": 12244, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTAGAACTGAAAGATTAAACGAATTGTTTTTTGTTTATCATAAAAACTTAAAAAATCAATCTAAATCTAAATATAGCAGAGCAGTTAATTACTTAAAAAGGCGTGGATTCAATTTACAAGATTTTTTAAAAGTTGGGGGTGGTGTTGGTTATTTACAAAATAAAGAATGA", "aa": "MDVRTERLNELFFVYHKNLKNQSKSKYSRAVNYLKRRGFNLQDFLKVGGGVGYLQNKE", "aa_hexdigest": "a751731d7e098c9c81701eadb454df60", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6898.821600000003, "isoelectric_point": 10.070106697082519 }, "id": "KKNAAMEMLJ_60", "locus": "GCA000027325_00018" }, { "type": "cds", "sequence": "contig_1", "start": 13104, "stop": 13331, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATGTTTGATCTCGTCAGCACTATTAACTAAATGTACATCTTTTCCACCGTGTGAATTACGTTTCTTAACGATGACAGGAAATGATTTGATTGTTTCTTTACTAAGAAGAGAAGAATTGACAGTTAGAAATCTATTTTGTTTTAATCATTTATATGTTTCGTATTTATCGTTTGCTATAAAAACAACATTAAAAGGATTAACTAAAAAAGTATTTATTTGA", "aa": "MFKCLISSALLTKCTSFPPCELRFLTMTGNDLIVSLLRREELTVRNLFCFNHLYVSYLSFAIKTTLKGLTKKVFI", "aa_hexdigest": "e7f7e74a783d38a9418c4f0ee17971e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8640.33959999999, "isoelectric_point": 9.505879402160645 }, "id": "KKNAAMEMLJ_61", "locus": "GCA000027325_00019" }, { "type": "cds", "sequence": "contig_1", "start": 13256, "stop": 13564, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAAATTAAATTAAAAAACAGAAAAGCATTAGTTGTTTATGACAACAAGGATGATTTTGAAAAAAATCAAACATTTGCTTTAAGTCTAATCAAGGAATTACAAAAGAAAAAACTAAATGCTGAAGTCTTATTACTTGAAAATAAAGACATTAATTTCGAAGCTAAAATTAATGAAGCTGAATTGATTTTAAATCGTTCAAGAAAAGTAGATTTTTTAAAAACCAATAATCAAATAAATACTTTTTTAGTTAATCCTTTTAATGTTGTTTTTATAGCAAACGATAAATACGAAACATATAAATGA", "aa": "MGKIKLKNRKALVVYDNKDDFEKNQTFALSLIKELQKKKLNAEVLLLENKDINFEAKINEAELILNRSRKVDFLKTNNQINTFLVNPFNVVFIANDKYETYK", "aa_hexdigest": "b8e1a2b672995e11f91a7ff849cdcef4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12009.794899999983, "isoelectric_point": 9.425422859191894 }, "id": "KKNAAMEMLJ_62", "locus": "GCA000027325_00020" }, { "type": "cds", "sequence": "contig_1", "start": 13569, "stop": 14057, "strand": "-", "frame": 1, "gene": "lysX", "product": "putative protein MG012", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0036211", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75097", "UniRef:UniRef90_P47258" ], "nt": "ATGCTCGATGTTTTTATTAATCAAATAAAACAAAGTATGGAGTTTCCTGTTATTGTTAAAAGTGTTTTTGGTTCTTTTGGTGATTATGTTTTTTTGTGTTTAGATGAACAAAAATTAAGAAAAACTTTAATGTCTTTTAATCAACAAGCAATTGTGCAAAAATACATTACTTGCTCTAAAGGTGAATCGGTAAGAGTTATTGTTGTGAACAATAAAGTTATAGGTGCTTTACATACAACTAATAATAGTGATTTTCGTTCTAATCTCAATAAAGGGGCAAAGGCAGAACGCTTTTTTTTGAATAAGGAACAAGAAAATTTAGCAGTTAAAATTAGTAAAGTAATGCAACTTTTTTATTGCGGTATTGATTTTTTGTTTGATCAAGACAGATCATTGATCTTTTGTGAAGTAAATCCTAATGTGCAATTAACAAGAAGCTCAATGTATTTAAATACTAATCTTGCAATTGAGCTTTTAAAAGCAATTTAG", "aa": "MLDVFINQIKQSMEFPVIVKSVFGSFGDYVFLCLDEQKLRKTLMSFNQQAIVQKYITCSKGESVRVIVVNNKVIGALHTTNNSDFRSNLNKGAKAERFFLNKEQENLAVKISKVMQLFYCGIDFLFDQDRSLIFCEVNPNVQLTRSSMYLNTNLAIELLKAI", "aa_hexdigest": "e7242341018933fd7eacc87b25ad2c9f", "start_type": "ATG", "rbs_motif": null, "genes": [ "lysX" ], "truncated": "3-prime", "pseudogene": { "start": 13569, "stop": 14357, "observations": { "mutation": [ 14069, 14219 ], "stop": [ 14069, 14219 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47258", "query_cov": 1.0, "subject_cov": 0.5644599303135889, "identity": 1.0, "score": 316.0, "evalue": 2.55e-107, "gene_start": 1, "gene_end": 162, "reference_start": 126, "reference_end": 287, "reference_sequence": "MKKINVVYNPAFNPISSKLNQTQLLKNASEELDIELKFFTSFDINTTKAKANLPFISNKILFMDKNIALARWLESNGFEVINSSIGINNADNKGLSHAIIAQYPFIKQIKTLLGPQNFDREWNPVMLDVFINQIKQSMEFPVIVKSVFGSFGDYVFLCLDEQKLRKTLMSFNQQAIVQKYITCSKGESVRVIVVNNKVIGALHTTNNSDFRSNLNKGAKAERFFLNKEQENLAVKISKVMQLFYCGIDFLFDQDRSLIFCEVNPNVQLTRSSMYLNTNLAIELLKAI" }, "paralog": false, "identity": 0.9923664122137404, "score": 506.0, "evalue": 7.97e-185, "description": "Internal stop codon at 14069, 14219. Nonsense mutation around 14069, 14219.", "psc": { "uniref90_id": "UniRef90_P47258", "gene": "LysX", "product": "Uncharacterized protein MG012", "uniref50_id": "UniRef50_P75097", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47258", "UniRef:UniRef50_P75097", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75097", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75097" ], "product": "Uncharacterized protein MG012 homolog" } }, "id": "KKNAAMEMLJ_63", "locus": "GCA000027325_00021" }, { "type": "cds", "sequence": "contig_1", "start": 14217, "stop": 14432, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAAACGTTGTTTACAATCCAGCATTTAATCCAATTAGCTCTAAATTAAATCAAACTCAACTTTTAAAAAATGCTAGTGAAGAGTTAGATATAGAACTAAAATTCTTTACTAGTTTTGATATTAATACAACTAAAGCAAAAGCAAATTTACCCTTCATATCCAACAAAATTCTTTTTATGGATAAAAATATTGCTTTAGCTAGATGA", "aa": "MKKINVVYNPAFNPISSKLNQTQLLKNASEELDIELKFFTSFDINTTKAKANLPFISNKILFMDKNIALAR", "aa_hexdigest": "508ea9bc6fc9432f5b22eb89e5f17be6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8105.386799999993, "isoelectric_point": 9.631657218933103 }, "id": "KKNAAMEMLJ_64", "locus": "GCA000027325_00022" }, { "type": "cds", "sequence": "contig_1", "start": 14503, "stop": 15138, "strand": "-", "frame": 3, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "GO:0000105", "GO:0004477", "GO:0004488", "GO:0005829", "GO:0006164", "GO:0009086", "GO:0035999", "SO:0001217", "UniRef:UniRef50_P47259", "UniRef:UniRef90_P47259" ], "nt": "GTGATTATTCAAGCTAATGATGATGATTCATCAGACAGTTTTATTAAACAAAAACTTATTGCTTGTAACACTGTAGGAGCAAAAAGTGAATTAATTAAACTATCTAATCAAATAACTCAAGCAGAGTTAATAGAAAAAATAATTAGTTTAAATCATGATGTAAATGTTACTGGTATCATTTTGCAATTGCCAGTTTATCCACACTTAGATAAAAACTCACTACTAGAAGCAATTAATCCTTTAAAAGATGTTGATGGTTTAACAACTAATCATTTGGCTGAAATTAAACCTTGTATAGTTGAAGCTATAATAACACTAAAAGAACTATTTAACCTTGAATTTAATAATCAAAAAATTGTTGTGGTAGGTTTGGGAATAACTGGTGGCAAACCTATTTATGAATTTTTAAAAACTAGTGGTTATAAAGTTCAAGCATGCGATAAAGATACTCCAAATACATTTGAATTGATTAAAAGTGCTGATATAGTTTTTACTGCTATTGGAAAATCTCATTTTTTTCAAGCTAAAAACTTTAAAAAAGGAGTTATTTTATTTGATATAGGTGTTTCAAGAAACAAGCAAAATAAACTTTGTGGTGATATAAATCCTGAAGGCATTGAAAAAAAAGCTAGATGA", "aa": "MIIQANDDDSSDSFIKQKLIACNTVGAKSELIKLSNQITQAELIEKIISLNHDVNVTGIILQLPVYPHLDKNSLLEAINPLKDVDGLTTNHLAEIKPCIVEAIITLKELFNLEFNNQKIVVVGLGITGGKPIYEFLKTSGYKVQACDKDTPNTFELIKSADIVFTAIGKSHFFQAKNFKKGVILFDIGVSRNKQNKLCGDINPEGIEKKAR", "aa_hexdigest": "422cef830fe9d044569da2be86c1fe31", "start_type": "GTG", "rbs_motif": null, "genes": [ "folD" ], "truncated": "both", "pseudogene": { "start": 14395, "stop": 15216, "observations": { "mutation": [ 14442, 14502, 14505, 15156 ], "stop": [ 14442, 14502, 14505, 15156 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47259", "query_cov": 1.0, "subject_cov": 0.7728937728937729, "identity": 0.995, "score": 412.0, "evalue": 9.95e-145, "gene_start": 1, "gene_end": 211, "reference_start": 27, "reference_end": 237, "reference_sequence": "MSFDGKLKAQSILETYKNFDWSKCKLVIIQANDDDSSDSFIKQKLIACNTVGAKSELIKLSNQITQAELIEKIISLNHDVNVTGIILQLPVYPHLDKNSLLEAINPLKDVDGLTTNHLAEIKPCIVEAIITLKELFNLEFNNQKIVVVGLGITGGKPIYEFLKTSGYKVQACDKDTPNTFELIKSADIVFTAIGKSHFFQAKNFKKGVILFDIGVSRNKQNKLCGDINPEGIEKKARWWTKTPGGVGPFTVLAIIKNLWILHEKNKRCLQSSI" }, "paralog": false, "identity": 0.9853479853479854, "score": 525.0, "evalue": 1.09e-191, "description": "Internal stop codon at 14442, 14502, 14505, 15156. Nonsense mutation around 14442, 14502, 14505, 15156.", "psc": { "uniref90_id": "UniRef90_P47259", "gene": "folD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_P47259", "cog_id": "COG0190", "cog_category": "H", "go_ids": [ "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47259", "UniRef:UniRef50_P47259", "COG:COG0190", "COG:H", "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_P47259", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47259" ], "product": "Bifunctional protein FolD" } }, "id": "KKNAAMEMLJ_65", "locus": "GCA000027325_00023" }, { "type": "tRNA", "sequence": "contig_1", "start": 15294, "stop": 15369, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 68.8, "nt": "GGGGATGTAGCTCAACTGATAGAGCACCTGATTTGCACTCAGGAGGTTGAGGGTTTGAGACCCTTCATCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 15334, 15336 ], "id": "KKNAAMEMLJ_36", "locus": "GCA000027325_00024" }, { "type": "tRNA", "sequence": "contig_1", "start": 15375, "stop": 15451, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 84.3, "nt": "GGAAGTATAGCTCAGTTGGTTAGAGCACACCCCTGATAAGGGTGAGGTCGATGGTTCAAGTCCATTTACTTCCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 15415, 15417 ], "id": "KKNAAMEMLJ_35", "locus": "GCA000027325_00025" }, { "type": "cds", "sequence": "contig_1", "start": 15555, "stop": 16310, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B41887", "UniRef:UniRef90_UPI0001B41887" ], "nt": "ATGGGGCTTGTTTTAAAAGAATTTAACAATAAAATAAGAACAGCATTAATTCTCGCTCCTTTTTTCACTTTCGCTCAGATAGTGATTGATTTAATCATTCCTTCTTTTTTAGCAAGTGCTATTTCAGTTGTGTTTTCAATTGATAAATTAAAACAGGATGAGTCAGGCGGAAAGACAATTTCAGTTGATTTTATTGGTGGGGCTAATATTAATTTTGCTAATGTAAGAGAAGCGCAAATAGTTTTAGCAACCACAGTTATTTTACTTGCATTATGTGGACTTTTTTTTGGTCTAATATCTATTTATTGTGCAAGTTATGTTTCAGCAAACACTTCTTTTCTATTAAGAAAAAAGATCTTTGCAAAATTAATGCGAATCACAACCCCATCACATGACCATTATGGTTCTTCTACTTTATTAGTTAGATTAACAAATGATGTTTATTTGATGGAGGTAATTGCTTTTGATTTTTTAAGGCTAATTATTCGTGCTCCGCTTTTGTTTATAGGTGGATTAGTGTTTGCAGTAACTACAAATCAAGATATGTCAATATCTTTACTAATTACTTTTCCTTTGATTCTTTTGGTAATTGGTATTCTAAACCGTAAATCTATTCCTTTATTTAAAGAAAATCAAAAATCAGTTGATAAGATAAATGAAAGAGTAGAAGAAGATGTTTCAGGTTATAAAGTAATTCAATCTTTTAATCTTCATTCCTTTACAAATAACAAATTTAAGATTGCAAATGAAGGATGA", "aa": "MGLVLKEFNNKIRTALILAPFFTFAQIVIDLIIPSFLASAISVVFSIDKLKQDESGGKTISVDFIGGANINFANVREAQIVLATTVILLALCGLFFGLISIYCASYVSANTSFLLRKKIFAKLMRITTPSHDHYGSSTLLVRLTNDVYLMEVIAFDFLRLIIRAPLLFIGGLVFAVTTNQDMSISLLITFPLILLVIGILNRKSIPLFKENQKSVDKINERVEEDVSGYKVIQSFNLHSFTNNKFKIANEG", "aa_hexdigest": "d012915d6df7302d863a526d83180f26", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 15555, "stop": 16613, "observations": { "mutation": [ 16308 ], "stop": [ 16308 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B41887", "query_cov": 1.0, "subject_cov": 0.6519480519480519, "identity": 1.0, "score": 462.0, "evalue": 8.6e-162, "gene_start": 1, "gene_end": 251, "reference_start": 1, "reference_end": 251, "reference_sequence": "MGLVLKEFNNKIRTALILAPFFTFAQIVIDLIIPSFLASAISVVFSIDKLKQDESGGKTISVDFIGGANINFANVREAQIVLATTVILLALCGLFFGLISIYCASYVSANTSFLLRKKIFAKLMRITTPSHDHYGSSTLLVRLTNDVYLMEVIAFDFLRLIIRAPLLFIGGLVFAVTTNQDMSISLLITFPLILLVIGILNRKSIPLFKENQKSVDKINERVEEDVSGYKVIQSFNLHSFTNNKFKIANEGWKKNSTSSLFINSLNIPFTFFLSSLTIIIALLLVFQLDSSVSVDPLPQDAAIRPNIFAFFQYNFYIVLGFILTSLTMVNFNRSRVALGRIKDILSQPEIKTITNKDQKELLPTLEFRNISFGLGNKNNNNFFTK" }, "paralog": false, "identity": 0.9971590909090909, "score": 666.0, "evalue": 6.19e-245, "description": "Internal stop codon at 16308. Nonsense mutation around 16308.", "psc": { "uniref90_id": "UniRef90_UPI0001B41887", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI0001B41887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B41887", "UniRef:UniRef50_UPI0001B41887" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B41887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B41887" ], "product": "ABC transporter permease" } }, "id": "KKNAAMEMLJ_66", "locus": "GCA000027325_00026" }, { "type": "cds", "sequence": "contig_1", "start": 16536, "stop": 16967, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGTGAACTTTAATCGTTCTAGAGTTGCACTAGGCAGAATTAAAGATATTTTATCTCAGCCTGAAATAAAAACTATTACAAATAAAGATCAAAAAGAATTATTACCTACCTTGGAGTTTAGAAATATCTCTTTTGGTCTTGGCAATAAGAACAATAACAATTTTTTACAAAATCTTAGTTTTAAATTTGAAGCATATAAAACATATGGTATTGTTGGGCCTACAGGCTCTGGAAAATCTTTAATTGCTAACATTATCGGTGGTTTATATGAGCCTAATGAGGGTGAAATTCTCATTGGTGGGGAGAAAATTCAATCTATTGACAGTTTATATCTATCAGAAATGATAGGAATTGTTTTCCAACAAAACATCCTTTTTAAAGGAACAATTTCTTCTAATATAAAAATTGGTATTGAAACTAGAAGTGATTGA", "aa": "MVNFNRSRVALGRIKDILSQPEIKTITNKDQKELLPTLEFRNISFGLGNKNNNNFLQNLSFKFEAYKTYGIVGPTGSGKSLIANIIGGLYEPNEGEILIGGEKIQSIDSLYLSEMIGIVFQQNILFKGTISSNIKIGIETRSD", "aa_hexdigest": "67a4154d88c79d2e2ace51672c06c0d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 76, "aa_cov": 0.5244755244755245, "hmm_cov": 1.0, "evalue": 4.2217928987648756e-17, "score": 58.12670135498047, "start": 56, "stop": 130 } ], "seq_stats": { "molecular_weight": 15873.016799999996, "isoelectric_point": 8.858745765686034 }, "id": "KKNAAMEMLJ_67", "locus": "GCA000027325_00027" }, { "type": "cds", "sequence": "contig_1", "start": 17001, "stop": 17426, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "nt": "ATGAAAAACGCAGCTAAAATAGCTTGCGCTGACACCTTTATTGAAAAGTTTTCTGATAGTTATGATCACAATGTTGAACAGTTAGGTAAAAACTTATCTGGTGGACAAAAACAAAGAGTTGCTATTGCAAGAACTTTAATTACAAAACCAAGAATTTTAGTTTTTGATGATAGTATGAGTGCTCTTGATGCTCTAACTGAAAAAAAAGTAAGAGAAAATATTGAAAATGATTTAAAGCTAACTACCAAAATCATTATTAGTCAAAACATTAATTCAATTAAACACGCAGATAAAATTTTGGTAATTGATAATGGCAGAATTGTTGGTTTTGATAGTGATCAAAAGCTAATGAAAAATTGTTCACTTTATCAAAAGATGAAAGAATCTCAAAAAGATTTGGGAGGTGATTTTGATGCAGTTAACTAG", "aa": "MKNAAKIACADTFIEKFSDSYDHNVEQLGKNLSGGQKQRVAIARTLITKPRILVFDDSMSALDALTEKKVRENIENDLKLTTKIIISQNINSIKHADKILVIDNGRIVGFDSDQKLMKNCSLYQKMKESQKDLGGDFDAVN", "aa_hexdigest": "4b00981fe19cc59148815d4e1c1e9d57", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DF8197", "query_cov": 0.9219858156028369, "subject_cov": 0.8441558441558441, "identity": 0.546, "score": 140.0, "evalue": 3.06e-40, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DF8197" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DF8197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "KKNAAMEMLJ_68", "locus": "GCA000027325_00028" }, { "type": "cds", "sequence": "contig_1", "start": 17512, "stop": 17784, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTATAGTTCTTGGTATTTTATATGGAATTGCTAACCCTATCTTATTAGCACAAGGTCTTGGTTTTATTTTTCCTATTACTAGTAGTAATGGTCGTGCTGTTGACTCAATATATTCATTAATTTACCCAACAAATTTAAATGTATTCATTAGGCTCACAATTGTGAGCGTAACTGTTTTTGTAGCTTATGCATTAATCTTTGTATTTAATGTAGCGCAAAACTATGTAGGGATTAAACTTTACCAACAAACATGTGCTACTTTGCGTTGA", "aa": "MCIVLGILYGIANPILLAQGLGFIFPITSSNGRAVDSIYSLIYPTNLNVFIRLTIVSVTVFVAYALIFVFNVAQNYVGIKLYQQTCATLR", "aa_hexdigest": "6f7dc25746710abec8f20da034aa2610", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9893.671699999995, "isoelectric_point": 9.137958335876466 }, "id": "KKNAAMEMLJ_69", "locus": "GCA000027325_00029" }, { "type": "cds", "sequence": "contig_1", "start": 17800, "stop": 18414, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAGTATGTCAACCAGCTTTTTTGATACGCAAAATAATGGTGATCTTATGAGTAGGTTAACTAATGATATGTATAACATTGATAACCTATTCACTCAAGCTGGTGGACAAGCTATTCAAAGTTTGTTTAATATTTTAACAACCTCAGTATTAATATTTTTATTAAGCCCAGTTATTGCACTTATTTCACTTTCAATTTTAGCTACATTAATTACTTTTTCTTTTGCCTTTCTAAAGAAATCAAAAACTTCATATAGTCAAGTACAAAATAATTTGGGTGATATGTCTGGTTATATTGAAGAGGTTTTAACTAATCATAAGGTTGTTCATGTCTTGAAGTTGCAAGAGATAATGATTAAGGATTTTGATCAATACAACAAATCAATGATCAAACCAACTGTAAGAGGGAATACATATTCGATCTTTCTTTTTTCTTGGTTTGGTTTTATATCAAATATTACTTATCTGGTTTCTATATCAATTGCTACTGCTTTTAGTGTTAATTCTATTCCTTCATTTGGAATTAGTGTTATTAACTATTCATTCATGTTGTCTTACATTGCTTCTTTAAGGCAAATAACTTTAGCATTAGATCAAATCTTTACCCTTTGA", "aa": "MQSMSTSFFDTQNNGDLMSRLTNDMYNIDNLFTQAGGQAIQSLFNILTTSVLIFLLSPVIALISLSILATLITFSFAFLKKSKTSYSQVQNNLGDMSGYIEEVLTNHKVVHVLKLQEIMIKDFDQYNKSMIKPTVRGNTYSIFLFSWFGFISNITYLVSISIATAFSVNSIPSFGISVINYSFMLSYIASLRQITLALDQIFTL", "aa_hexdigest": "8faf67753c8466a8959003904e3ab122", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22909.30110000001, "isoelectric_point": 6.7110593795776365 }, "id": "KKNAAMEMLJ_70", "locus": "GCA000027325_00030" }, { "type": "cds", "sequence": "contig_1", "start": 18886, "stop": 19242, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7NT64" ], "nt": "ATGCGTTTACCAAAAGGATATGACACTTATATTTCCAATAAAGCAGATTATCTTTCTGTTGGTGAAAGGCAATTATTAACAATTGCCAGAGCAGTAATCCGTAATGCTCCAGTTTTGCTCTTAGATGAAGCAACTAGTTCAGTTGATGTCCATTCAGAAAAATTAATTCAAGAATCAATAGGAAGGTTAATGAAAAATAAAACTTCTTTTATAATTTCTCATCGTCTTTCAATTATTCGTGATGCAACATTAATAATGGTTATTAATGATGGTAAAGTACTTGAAATGGGTAATCATGATCAGCTGATGAAACAAAATGGATTTTATGCACGTTTAAAACAATCTTCGGTTCGTTAA", "aa": "MRLPKGYDTYISNKADYLSVGERQLLTIARAVIRNAPVLLLDEATSSVDVHSEKLIQESIGRLMKNKTSFIISHRLSIIRDATLIMVINDGKVLEMGNHDQLMKQNGFYARLKQSSVR", "aa_hexdigest": "5e64f46355b735f8f6a4435ac85fd7d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00131ED865", "query_cov": 0.9576271186440678, "subject_cov": 0.9338842975206612, "identity": 0.5660000000000001, "score": 136.0, "evalue": 2.19e-39, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A7X7NT64" }, "pscc": { "uniref50_id": "UniRef50_A0A7X7NT64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7NT64" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "KKNAAMEMLJ_71", "locus": "GCA000027325_00031" }, { "type": "cds", "sequence": "contig_1", "start": 19252, "stop": 19623, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04434.22" ], "nt": "ATGACTGTTGCTGAAATTAAAAAACTTGCATTAAATAATCAGGTATTTAATGAAGCAAAAGCGCTTTTAGAAAAAGGTAATGTTATTTTTCCAAAAAAATTCTTAAAGCGAAAGAAGATCATTATTGAAGTATTAGATGGTAAGGTTTTTAAGGTTCAAATTAATTTAAAAACCGCTGCTGCACACTTGGATTGTAGTTGCTCTAATGATAAGCAAAATTGCGTTCATATAATTGCAGCGCTTTTGAAATATAATGATCTAAAAAATCAAGATAACAAAGAATTTGACCTTAATAAAGCCGATAAATTAGAGTGCAAAGAAGTTGAAATTCTAATTGAAAATGTTAGCTTAGCAATTGTTAATGGCAGCTGA", "aa": "MTVAEIKKLALNNQVFNEAKALLEKGNVIFPKKFLKRKKIIIEVLDGKVFKVQINLKTAAAHLDCSCSNDKQNCVHIIAALLKYNDLKNQDNKEFDLNKADKLECKEVEILIENVSLAIVNGS", "aa_hexdigest": "91ce7bc8403e9eef42379ea6dc1b9554", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04434.22", "name": "SWIM", "length": 39, "aa_cov": 0.2926829268292683, "hmm_cov": 0.9743589743589743, "evalue": 1.1813960199817542e-08, "score": 29.489242553710938, "start": 50, "stop": 85 } ], "seq_stats": { "molecular_weight": 13824.102099999984, "isoelectric_point": 8.85700511932373 }, "id": "KKNAAMEMLJ_72", "locus": "GCA000027325_00032" }, { "type": "cds", "sequence": "contig_1", "start": 19825, "stop": 20736, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATATGAAAATAACAGTTTGCTTTTTAATTTAGATCAATTCCTAAGCCTAGTTAAGGAAGTTAAAAAACCTTCATTATTCTTATTAAATGAGGATAAAACTGACAATATTCTTTTCTTAAGAAGTCAACATAAAATCAATGGATTAAGCCACGTTTGTGGTTTTTTAAATAATAAGGTTTTTGATTTTGTATCCTACAATGAAAAAACTAAGCAAATTGTTTTACGCTTAGCTTATCTTAATAAGTTCACTGATTTTAAATTTCCATACAACATTAACATCTATAAACTTGCTTTTGGAGAAACATTATTTTTCCATTTTTTAATCCATTTAAAAATGAATGGTTTTAAAAACATCTTTTTTCAAAGTGATGTTGTTATTGTTAAGGAAAGTGAATATCTACCTAAGATGTTTTTGACTATTGAATTTAATACACAAAAAAACAAGTTTATAACTGATGCTTTTTTTAAATATAAGAATAAAAATAGCAATACTTTAACAACTGTTTACCCCCATCGCTATTACTTAGCTCAAAAAACTAATACATCAAACTTCAACCGTTTACTTTTTTATGAACAAGCACTACAAAGGTTTTATGAAGAATTATTTCAAATAGATTATTTAAGAAGATTTGAAAACATTCCCATTAAAGATAAGAATCAAATTGCGCTTTTTAAAACTGTTTTTGATGATTACAAAACCATTGATTTAGCAGAATTGAAACTTACAAGTAATCTCTTAAATTACAAACAGTTACATTTTTCAATAAGTGATATTAAAGCTTTAAAAATTGAAGATAGACAACTAAAAATTGAATTTAAAGCTGGCGGGATAGATCTAAAGTTAATTAAAAGTGTTTTAAGTAACTACTATAAGGGTAATGCTATTTGTATTGGTGAAGATGGTTGA", "aa": "MQYENNSLLFNLDQFLSLVKEVKKPSLFLLNEDKTDNILFLRSQHKINGLSHVCGFLNNKVFDFVSYNEKTKQIVLRLAYLNKFTDFKFPYNINIYKLAFGETLFFHFLIHLKMNGFKNIFFQSDVVIVKESEYLPKMFLTIEFNTQKNKFITDAFFKYKNKNSNTLTTVYPHRYYLAQKTNTSNFNRLLFYEQALQRFYEELFQIDYLRRFENIPIKDKNQIALFKTVFDDYKTIDLAELKLTSNLLNYKQLHFSISDIKALKIEDRQLKIEFKAGGIDLKLIKSVLSNYYKGNAICIGEDG", "aa_hexdigest": "fb7acd6a8e176beb0a5709700e99637a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 36003.21119999999, "isoelectric_point": 9.250906944274899 }, "id": "KKNAAMEMLJ_73", "locus": "GCA000027325_00033" }, { "type": "cds", "sequence": "contig_1", "start": 20743, "stop": 21177, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00176.29" ], "nt": "TTGAACGATGAAAATGCTAAAGCACTAATTTCATTTTGGAGTCAAATTGACTTAAGAAATGCTACTTGTGATGCTAATAACAATTTGCTTCTTGCTAAATACCACTTGTTTGAAGTTGTAGATACTATTAGTAAATACACTGATGTAACTAATTTATTAGATGAAAAAACAGCCTTACAATTAAAAATAGCTAGTGAAAATCAATTTCATCTTTCGTTAGATAATAACCAAATTAATAACTTACGCAAATATCAAAAAGAAGGAGTTAAATGGATAAGGGCATTAGAAGATAATCAGTTTGGTGGAATTTTAGCAGATGAAATGGGGTTAGGTAAAACTGCTCAAGTGATCTTTGCAATGCTGGATAGTTATCAATCAACAAAATCACTTTTACCTAGTTTAATTATCGTTCCAGCATCCTTACTCTTAAATTGA", "aa": "MNDENAKALISFWSQIDLRNATCDANNNLLLAKYHLFEVVDTISKYTDVTNLLDEKTALQLKIASENQFHLSLDNNQINNLRKYQKEGVKWIRALEDNQFGGILADEMGLGKTAQVIFAMLDSYQSTKSLLPSLIIVPASLLLN", "aa_hexdigest": "2f1ca9220bc4611b7188c1caec9439d5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 63, "aa_cov": 0.4097222222222222, "hmm_cov": 1.0, "evalue": 4.2584193826030177e-17, "score": 57.18943405151367, "start": 84, "stop": 142 } ], "seq_stats": { "molecular_weight": 16217.3373, "isoelectric_point": 5.07500591278076 }, "id": "KKNAAMEMLJ_74", "locus": "GCA000027325_00034" }, { "type": "cds", "sequence": "contig_1", "start": 21208, "stop": 21321, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAATAGTTACTGCTAATGGCAATTTTAAAGAACGCTCGCAGGTATATGAATCATTAAAAAATCAGATTTTGTTAATGAGTTTTAATGTCTTAAGAAGTGATATTAAATGA", "aa": "MKIVTANGNFKERSQVYESLKNQILLMSFNVLRSDIK", "aa_hexdigest": "7c29837c88ccd1f8c2be4a057a51fe78", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4316.0114, "isoelectric_point": 9.819711494445798 }, "id": "KKNAAMEMLJ_75", "locus": "GCA000027325_00035" }, { "type": "cds", "sequence": "contig_1", "start": 21577, "stop": 22128, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37", "PFAM:PF00176.29" ], "nt": "ATGAAAAAAACGAGTCCTTTTATTTTAAGAAGGACTAAAAATAAAGTTTTAAAAGAACTACCTAAAAAAATTATTACTGATATCTATGTTGAACTTAGTGAAGAACATCAAAAACTGTATGATAAACAAAAAACAGATGGTTTGAAGGAGATTAAAGAAAGTGATGCTAAAAATGCTTTAAATATCCTTAGTTTAATCTTGAAATTAAGGCATATTTGTAGCTTAGTTAAAGACAATGATGTTAATGATTTTGAAGATAATTCCAAAGCTAATGCAGCTTTAAACATTATTTATGAAGCACTTGAAAATAAACGCAAAGTTATTTTGTTTACTCAATTTTTAGATGTAATTGATTGTTTTAAGCAAACTTTAAAAAATCAAAAGATTGATCACCTGGTATTTGATGGTAGAAAAACTGTGAAGAATAGAAACACTATTATCCAGAAGTTCAATAGTGCTAAAGAACCTTGTGTGATGCTAGCTTCTTTAAAAGCTGGTGGAGTTGGTATTAACTTAACTGCTGCTGAAGTTGTTATTCATTTTGATGTGTGA", "aa": "MKKTSPFILRRTKNKVLKELPKKIITDIYVELSEEHQKLYDKQKTDGLKEIKESDAKNALNILSLILKLRHICSLVKDNDVNDFEDNSKANAALNIIYEALENKRKVILFTQFLDVIDCFKQTLKNQKIDHLVFDGRKTVKNRNTIIQKFNSAKEPCVMLASLKAGGVGINLTAAEVVIHFDV", "aa_hexdigest": "12b022c647741e6586ff330e1bf761d8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 90, "aa_cov": 0.48633879781420764, "hmm_cov": 0.9444444444444444, "evalue": 6.249294145927506e-16, "score": 53.7525749206543, "start": 94, "stop": 182 }, { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 74, "aa_cov": 0.3879781420765027, "hmm_cov": 1.0, "evalue": 2.5884513015298705e-11, "score": 38.20649337768555, "start": 4, "stop": 74 } ], "seq_stats": { "molecular_weight": 20911.2033, "isoelectric_point": 9.315826606750488 }, "id": "KKNAAMEMLJ_76", "locus": "GCA000027325_00036" }, { "type": "cds", "sequence": "contig_1", "start": 22389, "stop": 23558, "strand": "+", "frame": 3, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "EC:1.8.4.-", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085", "RefSeq:WP_009885921.1", "SO:0001217", "UniParc:UPI0000129589", "UniRef:UniRef100_P47265", "UniRef:UniRef50_P78004", "UniRef:UniRef90_P47265" ], "nt": "ATGGCTGCTGGTAAAAGGGATTATTATGAAGTTCTAGGGATATCTAAAAACGCTAGTTCTCAAGACATAAAAAGAGCTTTTAGAAAGCTTGCAATGCAATATCACCCCGATCGTCATAAAGCAGAAAATGAAACTACTCAAAAACAAAATGAGGAAAAGTTTAAAGAGGTTAATGAAGCATATGAAGTTCTAAGTGATGAAGAAAAACGTAAGCTTTATGACCAGTTTGGTCATGAAGGGTTAAATGCTTCTGGTTTTCATGAAGCAGGGTTTAATCCTTTTGACATCTTTAATAGTGTTTTTGGTGAGGGATTTTCCTTTGGAATGGATGGTGATTCACCATTTGATTTCATTTTTAATCGTTCTAAAAAACGTCAACAACAAATTGTTGTTCCCTATAACCTTGATATTGCTTTAGTAATTGAAATTAACTTTTTTGAAATGACTAATGGTTGCAACAAAACCATCAAATATGAAAGAAAAGTTTCATGTCATAGTTGTAATGGTTTTGGCGCTGAAGGCGGGGAAAGTGGATTGGATCTTTGTAAGGATTGTAATGGCAATGGTTTTGTTATTAAAAACCAACGTTCTATCTTTGGAACCATTCAATCCCAAGTCTTGTGTTCAACTTGCAATGGACAAGGAAAACAAATTAAAGTTAAGTGCAAAACTTGTCGTTCTAACAAATACACTGTTACCAATCAAATTAAAGAGATTAATATTCCAGCAGGAATGTATAGTGGTGAAGCTTTAGTTGATGAAAGTGGTGGTAATGAATTTAAAGGTCACTATGGAAAATTAATCATTCAAGTGAATGTATTGGCAAGTAAGATTTTCAAACGTAGTGATAATAATGTTATTGCCAATGTTTTAGTAGATCCAATGGTTGCTATAGTTGGTGGGGTAATTGAACTACCTACTCTTGAAGGGATTAAAGAATTTAATATTAGACCAGGCACTAAGAGTGGCGAACAGATTGTTATTCCTAACGGTGGGATTAAATTCTCAAAGAGTTTTAAAAGAAAAGCTGGGGACTTAATCATTATTATTAGTTATGCACGTCCTTGTGAATACACTAACTTAGAATTGAAAAAATTACGTGAGTTTATCAAACCTAATCAAGAGGTTAAACAATATTTAAATACTTTAAAAAATGAATACAAAACTTAA", "aa": "MAAGKRDYYEVLGISKNASSQDIKRAFRKLAMQYHPDRHKAENETTQKQNEEKFKEVNEAYEVLSDEEKRKLYDQFGHEGLNASGFHEAGFNPFDIFNSVFGEGFSFGMDGDSPFDFIFNRSKKRQQQIVVPYNLDIALVIEINFFEMTNGCNKTIKYERKVSCHSCNGFGAEGGESGLDLCKDCNGNGFVIKNQRSIFGTIQSQVLCSTCNGQGKQIKVKCKTCRSNKYTVTNQIKEINIPAGMYSGEALVDESGGNEFKGHYGKLIIQVNVLASKIFKRSDNNVIANVLVDPMVAIVGGVIELPTLEGIKEFNIRPGTKSGEQIVIPNGGIKFSKSFKRKAGDLIIIISYARPCEYTNLELKKLREFIKPNQEVKQYLNTLKNEYKT", "aa_hexdigest": "012e99a02019b8ef7851895a7b90921e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129589", "ncbi_nrp_id": "WP_009885921.1", "uniref100_id": "UniRef100_P47265", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129589", "RefSeq:WP_009885921.1", "UniRef:UniRef100_P47265" ] }, "ips": { "uniref100_id": "UniRef100_P47265", "uniref90_id": "UniRef90_P47265" }, "psc": { "uniref90_id": "UniRef90_P47265", "gene": "dnaJ", "product": "molecular chaperone DnaJ", "ec_ids": [ "1.8.4.-" ], "uniref50_id": "UniRef50_P78004", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085" ] }, "pscc": { "uniref50_id": "UniRef50_P78004", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78004" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "KKNAAMEMLJ_77", "locus": "GCA000027325_00037" }, { "type": "cds", "sequence": "contig_1", "start": 23638, "stop": 23838, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATATCCATGGCGGACCTGGTTCTGGTACTGATGAAGGATGTTTAAAGTATTTTGATCTTGAAACAACTTGGATTATTTTATTAGATCAAAGAGGTTGTGGTAAGAGTAAGACTAATGATATCTTTTATGAAAATAACACTGATAAATTAGTTAGTGATTTTGAAATTTTACGTCAAAAATTAAACATTAAAAACTGA", "aa": "MYIHGGPGSGTDEGCLKYFDLETTWIILLDQRGCGKSKTNDIFYENNTDKLVSDFEILRQKLNIKN", "aa_hexdigest": "c4a32d14922b18736394d9680fd99bef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7558.449799999998, "isoelectric_point": 5.14542942047119 }, "id": "KKNAAMEMLJ_78", "locus": "GCA000027325_00038" }, { "type": "cds", "sequence": "contig_1", "start": 23968, "stop": 24135, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTAGGAAAAATGTTTTATCCTTATGAACACCAACGCTTTATGGATAGTATTCCTAAAGCTTATCAGAACAGTTATGAACAAATTGTTAACTACTGTTATGATCAATTTCAAAATGGTGATGAATCAACCAAAGAAAAACTTGCTAAAGCTTGGGTGGATTGA", "aa": "MGLGKMFYPYEHQRFMDSIPKAYQNSYEQIVNYCYDQFQNGDESTKEKLAKAWVD", "aa_hexdigest": "4fa629ae8e0979194b861e4c5b6ce087", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6607.3088000000025, "isoelectric_point": 5.156797218322752 }, "id": "KKNAAMEMLJ_79", "locus": "GCA000027325_00039" }, { "type": "cds", "sequence": "contig_1", "start": 24214, "stop": 24468, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATGTCACTATGCAGTTAATAAAAGTTTTTTGGATGAAAACTTCATTCTAGATAACATTAGTGTTCTTAAAAATAAAAGTATTTATTTGGCTCATGGTAGATTTGATCTGATCTGTCCTTTATATCAACCATTAGCATTAAAACAAGCATTCCCTGAATTACAACTTTATGTAACCAATAATGCTGGTCATAGTGGTAGTGATGCTAATAATTTAGCAACTATAAAACACCTTTTAAAAACTTACCTTTAA", "aa": "MECHYAVNKSFLDENFILDNISVLKNKSIYLAHGRFDLICPLYQPLALKQAFPELQLYVTNNAGHSGSDANNLATIKHLLKTYL", "aa_hexdigest": "b972969db37f342178fedff9182e500c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9524.865399999991, "isoelectric_point": 6.8863508224487315 }, "id": "KKNAAMEMLJ_80", "locus": "GCA000027325_00040" }, { "type": "cds", "sequence": "contig_1", "start": 24468, "stop": 24710, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "nt": "ATGAAGCGTTGTTATATTACAACCCCTATCTACTACGCATCAGGTAAGCCACACATAGGTCATGCTTTTACCACTATTTTGGCGGATGTAATTAAGCGTTTTAAAATCCAAAACGGATATGAGGCTTTTTTGCTTGTTGGCAGTGATGAACATGGCAATAAAATAGAAAGTAAAGCTAAAAGTTTAAATTTAGATCCTAAAACATTTGTTGATATTAACGCTCAAGCTTTTAAGTTAATGTGA", "aa": "MKRCYITTPIYYASGKPHIGHAFTTILADVIKRFKIQNGYEAFLLVGSDEHGNKIESKAKSLNLDPKTFVDINAQAFKLM", "aa_hexdigest": "648efa85a2d31d25aea590fb5cab23b8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A093YS71", "query_cov": 0.95, "subject_cov": 0.9743589743589743, "identity": 0.513, "score": 82.0, "evalue": 5.85e-19, "valid": false, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "uniref50_id": "UniRef50_A0A093YS71" }, "pscc": { "uniref50_id": "UniRef50_A0A093YS71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein" }, "genes": [], "id": "KKNAAMEMLJ_81", "locus": "GCA000027325_00041" }, { "type": "cds", "sequence": "contig_1", "start": 25371, "stop": 25766, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATCTTTAAACAACGTTATCTCTCCAGAACAATTAATTGATCAATTTGGTGTTGATGGTACAAGATATTGTTTATTAAAAGAGATGCGTTTAGATAAAGATAATCGTTGTAGTGTTAGCATCTTAAAAGAGATTTATAATGCTGATCTTGCCAATAGTTTTGGAAACCATGTTTCACGTACTTTTGGCATGATTAAAAAGTATCTAAACGGCAAATTAGAATACCAAATTATTACTGATAATGCACTTCAAAAAATAATGATTTTAATAGATGAATCAATCGTTCAATTTGATCATTACTTTAACAGTTATGAATTTTATAGAGCGATTAATCTACTTTTAAAAATTGTTTTTGAATTAAGTAAATTAATTGATGATTTCAAACCATGA", "aa": "MSKSLNNVISPEQLIDQFGVDGTRYCLLKEMRLDKDNRCSVSILKEIYNADLANSFGNHVSRTFGMIKKYLNGKLEYQIITDNALQKIMILIDESIVQFDHYFNSYEFYRAINLLLKIVFELSKLIDDFKP", "aa_hexdigest": "e5e20d209fd73bcb2a13f01980cd2d8e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15297.531499999992, "isoelectric_point": 6.095095252990721 }, "id": "KKNAAMEMLJ_82", "locus": "GCA000027325_00042" }, { "type": "cds", "sequence": "contig_1", "start": 25770, "stop": 26006, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAAATCAGGAATTCTCACTTTTAAAACAACTACTTTTTACTTGTGTTAGGTGTGTGCAGGTATGCTATGTGTTGTTAACACCTATCTTAGTAAATACTGCTTCAAAAGTTTTTCATTTATTTAATTTCGCTGATGATGCCTGTAGAAAAGATCAATTAAGAGATGCAACTTTATTAAAAAAAATTATTATCTCTAATTCAATGGAAGTTTTATTTAAAAGAGTAGATTAA", "aa": "MFKNQEFSLLKQLLFTCVRCVQVCYVLLTPILVNTASKVFHLFNFADDACRKDQLRDATLLKKIIISNSMEVLFKRVD", "aa_hexdigest": "6f5655566eed17e86cdd8dfc17e96edd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9085.770299999996, "isoelectric_point": 9.070653343200682 }, "id": "KKNAAMEMLJ_83", "locus": "GCA000027325_00043" }, { "type": "cds", "sequence": "contig_1", "start": 26036, "stop": 26128, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAGAGTACCTCAATCTTATTTCTCAAGCTAAAGTTATTGCAGAAAAACAATTTAAAGCTAACCCTTTTTCTTTTGAAACAATTTGA", "aa": "MQLEYLNLISQAKVIAEKQFKANPFSFETI", "aa_hexdigest": "dc9fa5bbd9bd6161886b243b351fd3a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3502.0416000000005, "isoelectric_point": 5.985566520690917 }, "id": "KKNAAMEMLJ_84", "locus": "GCA000027325_00044" }, { "type": "cds", "sequence": "contig_1", "start": 26261, "stop": 26473, "strand": "+", "frame": 2, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "COG:COG3343", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355", "KEGG:K03048", "SO:0001217", "UniRef:UniRef50_P47268", "UniRef:UniRef90_P47268" ], "nt": "ATGAAGTTTGATGAATGGAACAAGATATCACAATCTATGTTTGTTACAAAGGAGATTTTTGAAGAAGGTTATGAAGATCTTTCCAATAAAAAAGTAGAACCTGAGGAAGGAGTTGGTGATTTCATTATGGGAAATGATGGTGATGACAATGAAACTGGCAGTGAAATAGTACAAGGTTTAATTAATGATTCATTCAGTGAGGAAAATCAATAG", "aa": "MKFDEWNKISQSMFVTKEIFEEGYEDLSNKKVEPEEGVGDFIMGNDGDDNETGSEIVQGLINDSFSEENQ", "aa_hexdigest": "33251ba9ccc549576565c89ea720e8dd", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 26036, "stop": 26473, "observations": { "mutation": [ 26126, 26243 ], "stop": [ 26126, 26243 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47268", "query_cov": 1.0, "subject_cov": 0.4827586206896552, "identity": 1.0, "score": 139.0, "evalue": 5.57e-41, "gene_start": 1, "gene_end": 70, "reference_start": 76, "reference_end": 145, "reference_sequence": "MQLEYLNLISQAKVIAEKQFKANPFSFETIWKEVVKHFKISKQDEPSLIGRFYQDFLEDPNFVYLGDRKWKLRDFMKFDEWNKISQSMFVTKEIFEEGYEDLSNKKVEPEEGVGDFIMGNDGDDNETGSEIVQGLINDSFSEENQ" }, "paralog": false, "identity": 0.9862068965517241, "score": 287.0, "evalue": 6.06e-102, "description": "Internal stop codon at 26126, 26243. Nonsense mutation around 26126, 26243.", "psc": { "uniref90_id": "UniRef90_P47268", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47268", "cog_id": "COG3343", "cog_category": "K", "kegg_orthology_id": "K03048", "go_ids": [ "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47268", "EC:2.7.7.6", "UniRef:UniRef50_P47268", "COG:COG3343", "COG:K", "KEGG:K03048", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47268", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47268" ], "product": "Probable DNA-directed RNA polymerase subunit delta" } }, "id": "KKNAAMEMLJ_85", "locus": "GCA000027325_00045" }, { "type": "cds", "sequence": "contig_1", "start": 26500, "stop": 27030, "strand": "+", "frame": 1, "gene": null, "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "nt": "ATGCTTCAAAAAGCAAAGCTAGGTAAATATGCAATCCCTCACATTAACATCAATAACTATGAATGGGCCAAAGCTGTTTTAACAGCAGCAAATCAAGCTAATAGCCCAATTATTGTTTCAGTATCTGAAGGTGCTTTAAAGTACATGTCTGGTTATAGTGTTGTTATCCCGCTTGTTAAGGGTTTAATTGAATCACTAAGTGTTAAAGTACCAGTGACATTACATTTAGATCATGGTAGTTATGATGCATGTATCCAAGCATTACAGGCTGGATTTAGTTCAGTAATGTTTGATGGTTCACATTTACCATTTGAAGAAAATTTCAATAAATCTAAAAAGTTAATAGAGATAGCACAAAAAACAAATGCTTCTGTTGAACTTGAAGTTGGTACTATTGGTGGAGAAGAAGATGGTGTTATAGGACAAGGTGAGTTAGCTAATGTTGATGAATGTAAACAAATCGCTAGTTTAAAACCAGATGCTTTAGCAGCAGGAATTGGTAATATCCATGGTATCTATCCTAAGAATTGA", "aa": "MLQKAKLGKYAIPHININNYEWAKAVLTAANQANSPIIVSVSEGALKYMSGYSVVIPLVKGLIESLSVKVPVTLHLDHGSYDACIQALQAGFSSVMFDGSHLPFEENFNKSKKLIEIAQKTNASVELEVGTIGGEEDGVIGQGELANVDECKQIASLKPDALAAGIGNIHGIYPKN", "aa_hexdigest": "b04aa88ba169d0999907406be6cb5ac4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FEF401E", "query_cov": 0.9034090909090909, "subject_cov": 0.9034090909090909, "identity": 0.579, "score": 189.0, "evalue": 1.69e-58, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A3B0P7V3", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "product": "Fructose-bisphosphate aldolase" }, "genes": [], "id": "KKNAAMEMLJ_86", "locus": "GCA000027325_00046" }, { "type": "cds", "sequence": "contig_1", "start": 27049, "stop": 27345, "strand": "+", "frame": 1, "gene": null, "product": "fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "nt": "TTGATTGAAACAATATCAAAAATTACTAACTTACCCTTAGTTTTACATGGTGGCTCTGGAATCTTAGAAAATGATGTTAAAAAAGCAATTAGTTTAGGGATTTGCAAACTAAATATTAATACTGAGTGTCAATTAGCATTTGCACATGAAATTAGAAAATACATTGAATCAAATAAAGACTTGGATCTTAACAAAAAAGGTTATGATCCTAGAAAACTTTTAAAAGAACCTACTCAAGCAATTGTTGATACTTGCTTGGAAAAGATTGATTTGTGTGGTTCTAGAAATAAAGCATAG", "aa": "MIETISKITNLPLVLHGGSGILENDVKKAISLGICKLNINTECQLAFAHEIRKYIESNKDLDLNKKGYDPRKLLKEPTQAIVDTCLEKIDLCGSRNKA", "aa_hexdigest": "9cdc981a63c4f3b529d80113279022e0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A097ST37", "query_cov": 0.9795918367346939, "subject_cov": 0.9696969696969697, "identity": 0.635, "score": 124.0, "evalue": 3.83e-35, "valid": false, "product": "fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A097ST37", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A097ST37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "product": "fructose-bisphosphate aldolase" }, "genes": [], "id": "KKNAAMEMLJ_87", "locus": "GCA000027325_00047" }, { "type": "cds", "sequence": "contig_1", "start": 27346, "stop": 28233, "strand": "+", "frame": 1, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "COG:COG0012", "COG:J", "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47270", "UniRef:UniRef90_P47270" ], "nt": "ATGTTAAGTGCAGGGATAGTTGGTTTACCTAATGTTGGTAAGTCAACTTTATTTAGTGCTATTACTAATTTGCAAGTTGAAATAGCAAACTATCCTTTTGCAACTATAGAACCTAATACTGGCATTGTTAATGTTAGTGATGAGAGATTAGATAAATTAGCTAGCTTAATTAATCCTGAAAAGATAGTTTATACAACCTTTCGTTTTGTTGATATAGCAGGTCTTGTTAAAGGCGCCAGTCAAGGTCAAGGATTGGGTAATCAATTCTTAGCAAACATCCGTGAAGTGGATTTAATTTGTCATGTTGTAAGATGTTTTCAAGATAAAAAAATTGTTCATGTTAACAATACAATAGATCCTGTTTTTGATTTTGAAATTATTGTTAATGAACTAATCCAAGCTGATTTTGAATTAATAACAAACAGAATCGGCAAGCTTAAAAGAAAAGCTGAATCAGGTGATAAAATCGCTAAAGAAGAGTTTGTATTACTTGAAATTGTTTTAAATGGATTAAAACAAGGTCAAATGCCCATTCAGACTCTAAGTGAAAGTGAATTGAAAACAATTAAATCACTTAATCTATTAACAGCTAAACCTATTCTAATAGTAGCCAATGTATCTGAGAATGACTTATTAAACCTTGATAATAATGAAGCTTTAAAAAAATTGAATGCTTTTCTTGATCAAAAAAAGATTCCCAAGGCAATCACAGTTTGTTCTTTAATTGAAAAAGAATTAAGCGGTTTGAAATTAGAACAACGTCAATACTTTTTGGATGAACTTGGCTTAAAAAATTATTCAGGTTTAAACCGAGTAATTCAAGCTGCATATCAAACTTTAAACCTTTGGTCTTTTTTTACTTTTGGTAAAAAAGAAGTTAGAGCATGA", "aa": "MLSAGIVGLPNVGKSTLFSAITNLQVEIANYPFATIEPNTGIVNVSDERLDKLASLINPEKIVYTTFRFVDIAGLVKGASQGQGLGNQFLANIREVDLICHVVRCFQDKKIVHVNNTIDPVFDFEIIVNELIQADFELITNRIGKLKRKAESGDKIAKEEFVLLEIVLNGLKQGQMPIQTLSESELKTIKSLNLLTAKPILIVANVSENDLLNLDNNEALKKLNAFLDQKKIPKAITVCSLIEKELSGLKLEQRQYFLDELGLKNYSGLNRVIQAAYQTLNLWSFFTFGKKEVRA", "aa_hexdigest": "5bab8209b088d69e37c3bd4bb358615b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47270", "query_cov": 1.0, "subject_cov": 0.8038147138964578, "identity": 1.0, "score": 567.0, "evalue": 5.13e-203, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P47270", "cog_id": "COG0012", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47270" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "KKNAAMEMLJ_88", "locus": "GCA000027325_00048" }, { "type": "cds", "sequence": "contig_1", "start": 28342, "stop": 28449, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF06071.19" ], "nt": "ATGAAATCTTTACAAGAAGCTAAAAAACAAGGTTTGATAAGATTGGAAGGCAAAAATTACTTAATAAAAGATGGTGATGTTTGTAACTTTAAATTTAACGTCACTTAA", "aa": "MKSLQEAKKQGLIRLEGKNYLIKDGDVCNFKFNVT", "aa_hexdigest": "6ae3cf5b3cd0c8373b031a994816ed93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF06071.19", "name": "YchF-GTPase_C", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 3.393937513575871e-11, "score": 38.27375411987305, "start": 2, "stop": 33 } ], "seq_stats": { "molecular_weight": 4029.6841000000013, "isoelectric_point": 9.360438728332518 }, "id": "KKNAAMEMLJ_89", "locus": "GCA000027325_00049" }, { "type": "cds", "sequence": "contig_1", "start": 28652, "stop": 29305, "strand": "+", "frame": 2, "gene": "wcaA", "product": "putative glycosyltransferase MG025", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.-.-", "GO:0016757", "SO:0001217", "UniRef:UniRef50_P75086", "UniRef:UniRef90_P47271" ], "nt": "GTGCCAAGTAAGTATTTATTCACCGTTATAATTCCAACATACAATTGTTGTCAATACATAAAAAAGGCATTAGATTCATTGCTTTTACAGAATGAATATTTTTTAAAAACACAAGTACTAATAGTTAACGATGGTTCACTAGACAATACTAAGGAAGTTGTTAGTGACTATTTAATAAAGTACTCCAATATTAGTTATTTTGAAAAAACTAATGGTAATTGGGGTAGTGTTATTAACTATGTTAAAAAAAATAAATTAGCCTTAGGTCAATATATTACTGTTTTAGATAGCGATGATTATTTTTTAAAAGATAGTTTTAAAAAAGTGGCTCGTTTCTTTGGCCATGACATGATCATTGGCGCTTTTTATTGTTATATTAATGAAAATAAAACTCGTTTTTTAAAACCTTATTTCGGTAAGACTGGTGTTATTAAAGAACATACCAAATTAAGAACCCCCCATTCCCAACCTATTGCTAAATTTTACAGTAATAAACTTTTCTATGAACTACATGATCTTAAGGAAAAGTTATTCTTCCAGGATTGTTTAATGTATCACGATGCTATTAACAGAGTTGAAAGTGTTTTTTATTTGCGAGAGCCTTTAGCAGTTTGGTTTTCTACAAGACCTGGCAATTCTACAACAACTTCTTGA", "aa": "MPSKYLFTVIIPTYNCCQYIKKALDSLLLQNEYFLKTQVLIVNDGSLDNTKEVVSDYLIKYSNISYFEKTNGNWGSVINYVKKNKLALGQYITVLDSDDYFLKDSFKKVARFFGHDMIIGAFYCYINENKTRFLKPYFGKTGVIKEHTKLRTPHSQPIAKFYSNKLFYELHDLKEKLFFQDCLMYHDAINRVESVFYLREPLAVWFSTRPGNSTTTS", "aa_hexdigest": "e1b19815b773ebdece348878e0ee55b0", "start_type": "GTG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 28652, "stop": 29548, "observations": { "mutation": [ 29303, 29468 ], "stop": [ 29303, 29468 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47271", "query_cov": 1.0, "subject_cov": 0.7281879194630873, "identity": 1.0, "score": 445.0, "evalue": 4.95e-157, "gene_start": 1, "gene_end": 217, "reference_start": 1, "reference_end": 217, "reference_sequence": "MPSKYLFTVIIPTYNCCQYIKKALDSLLLQNEYFLKTQVLIVNDGSLDNTKEVVSDYLIKYSNISYFEKTNGNWGSVINYVKKNKLALGQYITVLDSDDYFLKDSFKKVARFFGHDMIIGAFYCYINENKTRFLKPYFGKTGVIKEHTKLRTPHSQPIAKFYSNKLFYELHDLKEKLFFQDCLMYHDAINRVESVFYLREPLAVWFSTRPGNSTTTSWENPNKFNAWCEILQKMNLYGAGIVIYIYTMLPGFLKQLKKKQLILNLNHKPAYTWLPKPLAFIFGGLMAFKTRKYIKYPK" }, "paralog": false, "identity": 0.9899328859060402, "score": 608.0, "evalue": 6.35e-224, "description": "Internal stop codon at 29303, 29468. Nonsense mutation around 29303, 29468.", "psc": { "uniref90_id": "UniRef90_P47271", "gene": "WcaA", "product": "Uncharacterized glycosyltransferase MG025", "ec_ids": [ "2.4.-.-" ], "uniref50_id": "UniRef50_P75086", "cog_id": "COG0463", "cog_category": "M", "go_ids": [ "GO:0016757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47271", "EC:2.4.-.-", "UniRef:UniRef50_P75086", "COG:COG0463", "COG:M", "GO:0016757" ] }, "pscc": { "uniref50_id": "UniRef50_P75086", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75086" ], "product": "Uncharacterized glycosyltransferase MG025 homolog" } }, "id": "KKNAAMEMLJ_90", "locus": "GCA000027325_00050" }, { "type": "cds", "sequence": "contig_1", "start": 29351, "stop": 29470, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTGTATGGAGCTGGGATAGTAATCTACATCTATACTATGCTACCTGGATTTCTAAAACAACTAAAGAAAAAACAACTAATACTGAATTTGAACCATAAACCAGCTTACACTTGA", "aa": "MNLYGAGIVIYIYTMLPGFLKQLKKKQLILNLNHKPAYT", "aa_hexdigest": "02c229a5a424075807cca34bce8f46eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4510.453400000001, "isoelectric_point": 9.903326988220215 }, "id": "KKNAAMEMLJ_91", "locus": "GCA000027325_00051" }, { "type": "cds", "sequence": "contig_1", "start": 29552, "stop": 30124, "strand": "+", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043", "KEGG:K02356", "RefSeq:WP_010869294.1", "SO:0001217", "UniParc:UPI0000129D0B", "UniRef:UniRef100_P47272", "UniRef:UniRef50_P75085", "UniRef:UniRef90_P47272" ], "nt": "ATGGCAGAAATGATAGAAGCAAAAAATCTTCGTAATGGGCAAACCATCTTCGGTCCTAACAAAGAGATTTTATTAGTACTGGAAAATACATTTAACAAAACCGCAATGCGCCAGGGAATTGTTAAAACTAAAGTTAAAAACTTAAGAACTGGGGCTATTGTTTGGCTTGAATTTACTGGTGACAAATTAGAACAAGTAATTATTGATAAGAAAAAAATGAATTTCTTATACAAAGATGGTAATAACTTTGTTTTTATGGATCAAAAAGACTACAGTCAGATTGAGATTAATGAAAAAAAATTAGAGTGGGAAAAAAATTTCATTACTGAAGAAATTGAAGTTACTGTTATTACTTATCAAGATGAAATTCTAGGAGTTAATTTACCTGATTTAGTTCCTATTGAAGTTGAGTTTGCTGAAGATGCTATTCAGGGCAATACTGCTAACATGGCAAGAAAAAAAGCACGCCTTGTAACTGGTTATGAACTTGATGTACCCCAATTTATTAATACTGGTGATAAGATTGTAATTGCCACTGTTGATGGCAATTACCGTGAAAGGTTTAACAAATAA", "aa": "MAEMIEAKNLRNGQTIFGPNKEILLVLENTFNKTAMRQGIVKTKVKNLRTGAIVWLEFTGDKLEQVIIDKKKMNFLYKDGNNFVFMDQKDYSQIEINEKKLEWEKNFITEEIEVTVITYQDEILGVNLPDLVPIEVEFAEDAIQGNTANMARKKARLVTGYELDVPQFINTGDKIVIATVDGNYRERFNK", "aa_hexdigest": "ef421207e07def004e20ce4e8c408cf3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129D0B", "ncbi_nrp_id": "WP_010869294.1", "uniref100_id": "UniRef100_P47272", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129D0B", "RefSeq:WP_010869294.1", "UniRef:UniRef100_P47272" ] }, "ips": { "uniref100_id": "UniRef100_P47272", "uniref90_id": "UniRef90_P47272" }, "psc": { "uniref90_id": "UniRef90_P47272", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_P75085", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ] }, "pscc": { "uniref50_id": "UniRef50_P75085", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75085" ], "product": "Elongation factor P" }, "genes": [ "efp" ], "id": "KKNAAMEMLJ_92", "locus": "GCA000027325_00052" }, { "type": "cds", "sequence": "contig_1", "start": 30093, "stop": 30260, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09185.15" ], "nt": "ATGGCAATTACCGTGAAAGGTTTAACAAATAAATTAACTAGAACCCAAAGACGAATTGCTGTAGTGGAATTTATCTTTTCTCTCCTCTTTTTCTTACCTAAAGAAGCTGAAGTTATTCAAGCAGATTTTTTAGAGTATGATACTAAAGAACGACAACTAAATGAATGA", "aa": "MAITVKGLTNKLTRTQRRIAVVEFIFSLLFFLPKEAEVIQADFLEYDTKERQLNE", "aa_hexdigest": "fd22dea1bdfd44b29cbd46360442dd47", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09185.15", "name": "DUF1948", "length": 44, "aa_cov": 0.8, "hmm_cov": 1.0, "evalue": 8.702487041481572e-25, "score": 82.33567810058594, "start": 12, "stop": 55 } ], "seq_stats": { "molecular_weight": 6475.4695999999985, "isoelectric_point": 6.225256538391113 }, "id": "KKNAAMEMLJ_93", "locus": "GCA000027325_00053" }, { "type": "cds", "sequence": "contig_1", "start": 30330, "stop": 30548, "strand": "+", "frame": 3, "gene": "nusB", "product": "putative protein MG027", "db_xrefs": [ "COG:COG0781", "COG:K", "SO:0001217", "UniRef:UniRef50_P47273", "UniRef:UniRef90_P47273" ], "nt": "TTGAAAAATCAATTAGAAATTCAAACTAAATACAATAAAATATCAGGAAAAAAGATTGATCTTTTAACTACTGCAGTTGTTTTATGTGCACTTAGTGAACAAAAGGCACATAATACTGATAAACCACTTTTAATTAGTGAGGCATTGTTGATTATGGATCATTATTCACAAGGTGCTGAAAAAAAACAAACTCATGCTTTATTAGATAAGCTCTTGTAA", "aa": "MKNQLEIQTKYNKISGKKIDLLTTAVVLCALSEQKAHNTDKPLLISEALLIMDHYSQGAEKKQTHALLDKLL", "aa_hexdigest": "98b0d1e966a6e47d2f9d8f098903e42f", "start_type": "TTG", "rbs_motif": null, "genes": [ "nusB" ], "truncated": "5-prime", "pseudogene": { "start": 30093, "stop": 30548, "observations": { "mutation": [ 30258 ], "stop": [ 30258 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47273", "query_cov": 1.0, "subject_cov": 0.4768211920529801, "identity": 0.986, "score": 140.0, "evalue": 1.79e-41, "gene_start": 1, "gene_end": 72, "reference_start": 80, "reference_end": 151, "reference_sequence": "MAITVKGLTNKLTRTQRRIAVVEFIFSLLFFLPKEAEVIQADFLEYDTKERQLNEWQKLIVKAFSENIFSFQKKIEEQQLKNQLEIQTKYNKISGKKIDLLTTAVVLCALSEQKAHNTDKPLLISEALLIMDHYSQGAEKKQTHALLDKLL" }, "paralog": false, "identity": 0.9933774834437086, "score": 286.0, "evalue": 1.61e-101, "description": "Internal stop codon at 30258. Nonsense mutation around 30258.", "psc": { "uniref90_id": "UniRef90_P47273", "gene": "NusB", "product": "Uncharacterized protein MG027", "uniref50_id": "UniRef50_P47273", "cog_id": "COG0781", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47273", "UniRef:UniRef50_P47273", "COG:COG0781", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_P47273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47273" ], "product": "Uncharacterized protein MG027" } }, "id": "KKNAAMEMLJ_94", "locus": "GCA000027325_00054" }, { "type": "cds", "sequence": "contig_1", "start": 30983, "stop": 31153, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAACTGCACATTTTTTTATTGATATAGAGATTTTGAAAGCCAAAAAAGGAGAACCCACTGATCCTAATAAAACTGATAATAGAGCAGTTGTTATTAATCTTGATGAGAATAAAAAGAATGAAAAAGAACAGTCCCCTCCTTCTGCAGAAATGACTAGTCTTTAA", "aa": "MSKTAHFFIDIEILKAKKGEPTDPNKTDNRAVVINLDENKKNEKEQSPPSAEMTSL", "aa_hexdigest": "54fd8293bb6a9a7188abedcdfb7798ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6300.047800000002, "isoelectric_point": 5.7142740249633786 }, "id": "KKNAAMEMLJ_95", "locus": "GCA000027325_00055" }, { "type": "cds", "sequence": "contig_1", "start": 31143, "stop": 31736, "strand": "-", "frame": 1, "gene": "yajL", "product": "putative protein MG029", "db_xrefs": [ "COG:COG0693", "COG:V", "GO:0005737", "SO:0001217", "UniRef:UniRef50_P47275", "UniRef:UniRef90_P47275" ], "nt": "ATGCTGGGGACCGTTTATTTGGTACTAAATAAAATGAAAAAGATACTTGTTATTGTCTATCCAGAGATGAATGATGTTGAATATACTAATGTAATGGTTGTATTTAGTTTCATTAAAACAATACAAACAACATGTTATCACCATTCATTAAAAAAGATAACTGCTAGTAATGGTGTTGTTGAAGTTAACAATATTGTCAACACCATTAACTTATCAGAATTTGATGCAGTTTATATTCCCGGTGGAATAGGAGCTACAAAACACTTAGATAAAGATGAAAAACTGCTTAAAACAATTAATTATTTCAAAGTAAATAACCTTTATCTTTTTGCTATTTGTGACACACCGAATGTTCTATTTAAACATGGTATTATCACTAAAGATGAGATCTATTCTTCTTTTCCAAACCCAAATTTAGTTATGTCTGAAAACAGATCAACAGCAAAAGTAACAGTAGCAAATAAATTAATAACAGCAAGAAGTGCTGGTTGTGCTTTGGAATTTGCAACAGTTATTGTGTGCACTTTTTTAAAAGATAACACTTTAAATGAGCTTGTTCATAAAAGGTTATTTGGTAGTGAAATTAAAGACTAG", "aa": "MLGTVYLVLNKMKKILVIVYPEMNDVEYTNVMVVFSFIKTIQTTCYHHSLKKITASNGVVEVNNIVNTINLSEFDAVYIPGGIGATKHLDKDEKLLKTINYFKVNNLYLFAICDTPNVLFKHGIITKDEIYSSFPNPNLVMSENRSTAKVTVANKLITARSAGCALEFATVIVCTFLKDNTLNELVHKRLFGSEIKD", "aa_hexdigest": "6f4c7a7c877f5790514d8340318d40ec", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47275", "query_cov": 0.9441624365482234, "subject_cov": 1.0, "identity": 1.0, "score": 369.0, "evalue": 4.24e-129, "valid": true, "gene": "YajL", "product": "Uncharacterized protein MG029", "uniref50_id": "UniRef50_P47275", "cog_id": "COG0693", "cog_category": "V", "go_ids": [ "GO:0005737" ] }, "pscc": { "uniref50_id": "UniRef50_P47275", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47275" ], "product": "Uncharacterized protein MG029" }, "genes": [ "yajL" ], "id": "KKNAAMEMLJ_96", "locus": "GCA000027325_00056" }, { "type": "cds", "sequence": "contig_1", "start": 31705, "stop": 32325, "strand": "-", "frame": 3, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0005737", "GO:0006223", "GO:0044206", "RefSeq:WP_009885912.1", "SO:0001217", "UniParc:UPI0000137D2A", "UniRef:UniRef100_P47276", "UniRef:UniRef50_P47276", "UniRef:UniRef90_P47276" ], "nt": "GTGATAAAAAAAGTTCAACATGCTTTAATCTTGAATGAATTGACAAAACTGCGTGATAAAAATACAACAACCTCCCAGTTTCGCATGGCCTTGAATCAAATCACTTCATTACTCTTTTTTGAAGCAACTAAACAGCTACCACTAGCAACAGTTGAAGTTGAAACTCCCTTTGCTAAAACAAAGGGCTACAAATTAAAAAATGACATTGTTCTTGTACCTATTATGCGTGCTGGACTTGGAATGATTGATGCTATTGTTCGCTATTCAGATAAAATCAGAGTTGGTCATTTAGGAATCTATCGTCAAACCCAAACAACCAGTGTAATTTCATACTATAAAAAGATGCCTGAAAACATCTCTGATTCACATGTTATTATTCTTGATCCTATGCTTGCTACTGGAACTACATTGTTAACTGCTATTAAATCTATTAAAGAAGATAAACCTATCAAAATTAGTGTTATTGCTATAGTAGCAGCACCTGAAGGAATTAATAAAGTAGAAAAAATGCATCCTCATGTTGATATATTTCTTGCAGCAATTGATGAAAAGTTAAATGACAATAGATACATAATCCCTGGTCTTGGTGATGCTGGGGACCGTTTATTTGGTACTAAATAA", "aa": "MIKKVQHALILNELTKLRDKNTTTSQFRMALNQITSLLFFEATKQLPLATVEVETPFAKTKGYKLKNDIVLVPIMRAGLGMIDAIVRYSDKIRVGHLGIYRQTQTTSVISYYKKMPENISDSHVIILDPMLATGTTLLTAIKSIKEDKPIKISVIAIVAAPEGINKVEKMHPHVDIFLAAIDEKLNDNRYIIPGLGDAGDRLFGTK", "aa_hexdigest": "9ae84fd6e1678687e79f4aa1b2b1c548", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137D2A", "ncbi_nrp_id": "WP_009885912.1", "uniref100_id": "UniRef100_P47276", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137D2A", "RefSeq:WP_009885912.1", "UniRef:UniRef100_P47276" ] }, "ips": { "uniref100_id": "UniRef100_P47276", "uniref90_id": "UniRef90_P47276" }, "psc": { "uniref90_id": "UniRef90_P47276", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_P47276", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0005737", "GO:0006223", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_P47276", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47276" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "KKNAAMEMLJ_97", "locus": "GCA000027325_00057" }, { "type": "cds", "sequence": "contig_1", "start": 32359, "stop": 32820, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12836.13", "PFAM:PF14579.12" ], "nt": "TTGAGTTACTATGCTTGTTTATTAAGTTTCAAACTAAAGGAACATGATATTAATGGTTTTGAAAAAGGGTATGAATTTATTAAAAACCGTTTAGATGAACTTAATAAGCTTTACAGAATAAAAAAGATTAAACCAAAAGAAGCGGAGCTTTTAACAAGTTATGAAGTTTATTTAGAAATGATGGCACGCAATATAAAGTTGCAACAAATCTCTATTCAAAACTCTAATGCAAGGATGTTTGTTGAACATAATGGTGTTTTAATTGCGCCTTTCATCACTATTCCAGGAATGGGAGAAGCGGTAGCTTCTTCAATTGTAGAAGCAAGAAATGAAAAACCATTTGCTTCCTTAAATGATTTCAAAAAAAGAACCAAAATTACTAAAAAACACGTTGAAACGATGGAACAATTACAACTGTTTGATGAATTTGAACATCAAGATGATCATAAACTTTTCAACTAA", "aa": "MSYYACLLSFKLKEHDINGFEKGYEFIKNRLDELNKLYRIKKIKPKEAELLTSYEVYLEMMARNIKLQQISIQNSNARMFVEHNGVLIAPFITIPGMGEAVASSIVEARNEKPFASLNDFKKRTKITKKHVETMEQLQLFDEFEHQDDHKLFN", "aa_hexdigest": "256d34ff6c898c33f9bb34d867885202", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12836.13", "name": "HHH_3", "length": 44, "aa_cov": 0.28104575163398693, "hmm_cov": 1.0, "evalue": 1.4919630865345365e-09, "score": 32.9807014465332, "start": 93, "stop": 135 }, { "id": "PF14579.12", "name": "HHH_6", "length": 85, "aa_cov": 0.5294117647058824, "hmm_cov": 0.9882352941176471, "evalue": 6.941981510094498e-17, "score": 56.59148406982422, "start": 65, "stop": 145 } ], "seq_stats": { "molecular_weight": 17992.598299999998, "isoelectric_point": 7.817001152038574 }, "id": "KKNAAMEMLJ_98", "locus": "GCA000027325_00058" }, { "type": "cds", "sequence": "contig_1", "start": 32851, "stop": 33054, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATCTAATTAATAAAGGGATGCAAGCAAAAGATGCTTTTGAAATCATGGAAAAAGTAAGAAAAGGTATTAAAGTAAATGCTAAAGAAGTTAGCCTGATGCAAAACTGTGGTGTTGAACAATATTGGATTAATGCTTGTTTAAAAATTAATTATCTATTCCCTAAGGCACATGCTGCAGCTTATGTTTTAATGGCTTGA", "aa": "MLYLINKGMQAKDAFEIMEKVRKGIKVNAKEVSLMQNCGVEQYWINACLKINYLFPKAHAAAYVLMA", "aa_hexdigest": "34910a29dfd04a42bf9e5e4df94ceae6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7654.1149000000005, "isoelectric_point": 9.230857276916506 }, "id": "KKNAAMEMLJ_99", "locus": "GCA000027325_00059" }, { "type": "cds", "sequence": "contig_1", "start": 33130, "stop": 33384, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGTTAAAGCACTTAGCTGATCTAACTAAGATAAATCCCCAAAATATCCCTCACTTTGATAAGAACTTAATTTCGATGTTTTCTTCAAACAAACCATTAAACCTAAAACCAGGCATTGTTGATGAAGTTACAGGAGCGGTTGGTATCCCTGAATTTGGGACTAAATTTGTTAGAAAAATTTTAGAACAGACAAAACCTAAAGACTTTGCTGACTTAATTAGAGTTTCAGGATTAAGCCATGGTAAAAACGTGTGA", "aa": "MLKHLADLTKINPQNIPHFDKNLISMFSSNKPLNLKPGIVDEVTGAVGIPEFGTKFVRKILEQTKPKDFADLIRVSGLSHGKNV", "aa_hexdigest": "6a9bef7ad3a19e47ef211790435f60e8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 88, "aa_cov": 0.9761904761904762, "hmm_cov": 0.8409090909090909, "evalue": 8.085875552572875e-18, "score": 59.38804626464844, "start": 2, "stop": 83 } ], "seq_stats": { "molecular_weight": 9308.783999999992, "isoelectric_point": 9.630883598327635 }, "id": "KKNAAMEMLJ_100", "locus": "GCA000027325_00060" }, { "type": "cds", "sequence": "contig_1", "start": 33463, "stop": 33897, "strand": "-", "frame": 3, "gene": null, "product": "DNA polymerase III alpha subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E0Y6" ], "nt": "ATGATTCGTGATTGTCCTAAATGCCATGAAAAAGCAAGTTTCAAAGGTGATGGTCATAACATTCCTTTTGCTACTTTTATGGGTTTTTCTGGTGATAAAATTCCTGATATCGATCTTAATTTTTCTAGCGAATATCAAGCTAAAGCCCATGATTATGTCAGAAAATTATTTGGAGTTAATAACACCTTCAGGGCAGGAACAATTGCAACAGTTGCTGAAAAAACTGCTTATGGTTATGCTAGAAACTATTTTGAAATCATTAAACGTGTTGATCTTGCAACTACTGCTGAAATTGAAAGATTTAAACAAAAGCTTATAGGAATTAAGAGAACAACAGGGCAACATCCAGGGGGAATTATGATCTTTCCTAGTGATCATTCTGTTTATGAATTTACTCCCTGTGGTTTTCCAGCAGATGATGTTGAAAGTGAATGA", "aa": "MIRDCPKCHEKASFKGDGHNIPFATFMGFSGDKIPDIDLNFSSEYQAKAHDYVRKLFGVNNTFRAGTIATVAEKTAYGYARNYFEIIKRVDLATTAEIERFKQKLIGIKRTTGQHPGGIMIFPSDHSVYEFTPCGFPADDVESE", "aa_hexdigest": "c63ff05b17895e59818e6c4bcc026882", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380E0Y6", "query_cov": 0.8402777777777778, "subject_cov": 0.9453125, "identity": 0.562, "score": 140.0, "evalue": 3.12e-40, "valid": false, "product": "DNA polymerase III alpha subunit", "uniref50_id": "UniRef50_A0A380E0Y6" }, "pscc": { "uniref50_id": "UniRef50_A0A380E0Y6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E0Y6" ], "product": "DNA polymerase III alpha subunit" }, "genes": [], "id": "KKNAAMEMLJ_101", "locus": "GCA000027325_00061" }, { "type": "cds", "sequence": "contig_1", "start": 34195, "stop": 34365, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAAAGAATGAGTTTTTTAGGAGAAGATATTGCCTATAAATTAGTCGTTGAAAACACTAATAAGATAGTGAAGCTACTTGACTTAAATGAATTAGTACCAACTAAAAACAAGCTTTATCCACCAGTGATGCAAGATTCTAACCAAAAATTAATTGATAAAACTTGA", "aa": "MLKRMSFLGEDIAYKLVVENTNKIVKLLDLNELVPTKNKLYPPVMQDSNQKLIDKT", "aa_hexdigest": "5ef16155b97e0ec6570415fb0ddf46ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6491.637900000003, "isoelectric_point": 9.049507713317869 }, "id": "KKNAAMEMLJ_102", "locus": "GCA000027325_00062" }, { "type": "cds", "sequence": "contig_1", "start": 34435, "stop": 35109, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCCTTGTTTTCATCCCATACTTTAAGCAATATTTGTTCTAAACTAAAACTTGAATTTGATGATGAAAGATTACACCGTGCTGAATATGATACAGAAGCTTTAAAAAAAGTTTTCTTTTACTTTAAAAAGCAGTTAAAAGAAATGGGTATTAATACCTTAACAGAGATAGATCAAAACCTAAATAAAAAGTGTCAAATTGATCTGATGAAAAGGGTATTTACAAACACAGCAATCGTTTATGTAAAAAACCAACGGGGCTTTCAAAACCTATATGAGATGTTATCAATTGCATTAACAGATCATAATGCTAACAGACCATTAGTTTTAGCTAGCAGCTTAGCTAAATTTAGAAAATCTTTTTTATTAACAGAAAACCCTGTTCAGGGTGACATCTTTAAAGCTGCATTAACAAAACCAATTAATGAACTTGAAAAAGCAATTGAAAAAGTTGACTTTGTGCTTATTTCACAACCCAATGCTTACCTTGGTTATACACTACGTGAAGGACTGAAAAAAGAACTTATTAATGATGCAATCAAACTAGTTATCAAAACAGCAACAAAACTTAAGAAATTAGTTGCTGTTGCATCTGATGCTTACTTTATCCATCCTTGGGAAAATGAATATTATAAGGCAATAGTTTGTGCTAAGGGCTTAGGTGGGAAATGA", "aa": "MNPLFSSHTLSNICSKLKLEFDDERLHRAEYDTEALKKVFFYFKKQLKEMGINTLTEIDQNLNKKCQIDLMKRVFTNTAIVYVKNQRGFQNLYEMLSIALTDHNANRPLVLASSLAKFRKSFLLTENPVQGDIFKAALTKPINELEKAIEKVDFVLISQPNAYLGYTLREGLKKELINDAIKLVIKTATKLKKLVAVASDAYFIHPWENEYYKAIVCAKGLGGK", "aa_hexdigest": "b05e5e06f8842947487e95cec938c3d0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25642.648900000022, "isoelectric_point": 9.30048313140869 }, "id": "KKNAAMEMLJ_103", "locus": "GCA000027325_00063" }, { "type": "cds", "sequence": "contig_1", "start": 35113, "stop": 35580, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "GTGGCTAAAAAATATGATTTAAAAGCTATCTATGGTTTGGAATTCAACTTAACTGATGATCACATCAAAATTGTTCACAACCCAGATAACACTAAGTTAAGTGATGCCACCTTTGTTATTTTTGATATTGAAACCACTGGATTACATGGTAGGTATGATGATGTTATTGAGTTTTCAGCACGCAAAATTAAGAATAACAGCGAGATAGATCATCAGCAATTCTTTTTAAAAATTGACAAACCTATCCCAAAAACAATCACTGAAATCACCAAAATAACTGATGAGATGCTTGAAGGCGGTATTGATCAACAGCAAGGTTTAGAAAAGATAAGAAATTATCTAGATGATTGTGTTATGGTAGCTCATAATGGTATTAATTTTGATTTACCCTTTTTGCAAACTCAATTTGAAAAATACAACATCAAACCATTAACAAACCCACTGATTGATACCTTATGTTTATCATGA", "aa": "MAKKYDLKAIYGLEFNLTDDHIKIVHNPDNTKLSDATFVIFDIETTGLHGRYDDVIEFSARKIKNNSEIDHQQFFLKIDKPIPKTITEITKITDEMLEGGIDQQQGLEKIRNYLDDCVMVAHNGINFDLPFLQTQFEKYNIKPLTNPLIDTLCLS", "aa_hexdigest": "049ac24d0929f877f3495ed2137092f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 112, "aa_cov": 0.7225806451612903, "hmm_cov": 0.8125, "evalue": 1.7242818763835706e-09, "score": 32.90215301513672, "start": 39, "stop": 150 }, { "id": "PF00929.31", "name": "RNase_T", "length": 124, "aa_cov": 0.7483870967741936, "hmm_cov": 0.9919354838709677, "evalue": 2.6163594835617704e-25, "score": 84.94076538085938, "start": 39, "stop": 154 } ], "seq_stats": { "molecular_weight": 17877.1915, "isoelectric_point": 5.01811008453369 }, "id": "KKNAAMEMLJ_104", "locus": "GCA000027325_00064" }, { "type": "cds", "sequence": "contig_1", "start": 35638, "stop": 35727, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGTTAGTTTTTCATACTAAAATGACTGCTTTTGATGGTATCAATGACATTGAAGAATATGCACAGTTTGCTAAAGAAAGAGGTTGA", "aa": "MELVFHTKMTAFDGINDIEEYAQFAKERG", "aa_hexdigest": "dcdc63ed0322925c47ba882e9f810bcd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3391.782, "isoelectric_point": 4.641665458679199 }, "id": "KKNAAMEMLJ_105", "locus": "GCA000027325_00065" }, { "type": "cds", "sequence": "contig_1", "start": 35902, "stop": 36195, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATGCTGGTTTTAAAAACTTTAACTTCATTGCACTTTATCCTATTGATAAAAATGAATCTAAATTAAAAGTAAAAGCTGTAACTGTTTCTCAATATGATAAGCAGTTTGAAACAAAGGTATTTGCAACAGAATTTATCCCTATCCACAAGATTAACCAACAGATCGATGATGTCAAGATTATTGGGCAAATCTTTGAATTAAAAACCCATGAAAGTTTAACTGGTAAAAAAACACTTAATATCTATGTAACTGATTTTCAATTAGGTGGATCGTTAATTTTGAAATGA", "aa": "MNNAGFKNFNFIALYPIDKNESKLKVKAVTVSQYDKQFETKVFATEFIPIHKINQQIDDVKIIGQIFELKTHESLTGKKTLNIYVTDFQLGGSLILK", "aa_hexdigest": "429e5eb910b192178112f6fa5c312b6e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11109.755599999991, "isoelectric_point": 9.156976509094239 }, "id": "KKNAAMEMLJ_106", "locus": "GCA000027325_00066" }, { "type": "cds", "sequence": "contig_1", "start": 36514, "stop": 36714, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTTAATCTTGAGAATGAAAAAGATAAAAAGGTTCTTGCACTCTCCAATTTTTTGATAGATAACAATATCCTTGACCACAATGAGCTTAACAGCTTAATTGAAAGGATTGAATCAATTTACTTTAATAAATATATTGAAGAGAAGGTTTTTCTATTTGCAATAACTATTAGTCGCCCTTTAACTATTGATATTTGA", "aa": "MVFNLENEKDKKVLALSNFLIDNNILDHNELNSLIERIESIYFNKYIEEKVFLFAITISRPLTIDI", "aa_hexdigest": "c41b364f725ab4630d87c3c3921fab6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7815.942699999991, "isoelectric_point": 4.750114250183105 }, "id": "KKNAAMEMLJ_107", "locus": "GCA000027325_00067" }, { "type": "cds", "sequence": "contig_1", "start": 36977, "stop": 37957, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078", "UniRef:UniRef90_P47278" ], "nt": "ATGAAAACAATTCATAAACTATTTTTAGGTCTTTGTTTACCCGCAACATTAGGTCCTTTACTTGGAATTGTTGTTACAAATACTGACCAAAGTATTAAGTTTACAAGCAAATCAAATTCGATTAATAAGAATAACCAAAATAAAGAGTTGGCTCTACTTAGAGATAATTTGATGAACGAAGCGAAAGTTGATGAACCACTTTCCTTTGAAAAACGGTTTGAAAACTTTAAAAATAAGTATAGTGATATACATAGCTTAAATAACAGTGTTTTTTCACTTCATGACGTTTATGACTTATTAGGTGGATTTAAACAATCATTGACAACATTTTTTGATGAAGTGATTGCCCAACAACAAAAGATCAAGGATGCAGATAAGATCTTTCCAAGTACTAAAGATAATCCACCTAAAGAAGAAAATCCTAATGTTTTAGATACACTAGCTAACTACCAAGGAGCAGGATTTTTCCCTAGTTTAGGTAAAAATGGTTTTAATTTACCTGAAGCAGTGTTCCAAAATTTCACTGATTTTAGGATTAATGACTACAAGATTAAAAATTTTAATGTTGATCTTGTTAGTGAAAATGACATTATTAAACATGATAAAGTTCGTTATGCTTTTGAAGTTAAGTTCAATATTGCTTTAGTTTTATCTATTAATAAGTCAAATGTTGATTTTGACTTTGATTTCATTTTAAAGACTGATAATTTCTCAGACATTGAAAACTTTAATGAAATTTTCAACAGAAAACCTGCTTTACAATTTCGTTTTTATACCAAGATCAATGTACATAAGTTAAGTTTCAATGGTAGTGATTCCACTTACATTGCCAATATCTTGTTACAAGATCAGTTCAACCTATTAGAAATTGATTTAAATAAATCTATTTATGCATTAGATCTTGAAAATGCTAAAGAACGCTTTGATAAGGAATTTGTTCAACCTCTTTATCAAAAACGACGTGAAGCAAAGCTTGCTTGA", "aa": "MKTIHKLFLGLCLPATLGPLLGIVVTNTDQSIKFTSKSNSINKNNQNKELALLRDNLMNEAKVDEPLSFEKRFENFKNKYSDIHSLNNSVFSLHDVYDLLGGFKQSLTTFFDEVIAQQQKIKDADKIFPSTKDNPPKEENPNVLDTLANYQGAGFFPSLGKNGFNLPEAVFQNFTDFRINDYKIKNFNVDLVSENDIIKHDKVRYAFEVKFNIALVLSINKSNVDFDFDFILKTDNFSDIENFNEIFNRKPALQFRFYTKINVHKLSFNGSDSTYIANILLQDQFNLLEIDLNKSIYALDLENAKERFDKEFVQPLYQKRREAKLA", "aa_hexdigest": "102a014d6024e4f5635855d1e1adc428", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 36977, "stop": 38260, "observations": { "mutation": [ 37955, 38237 ], "stop": [ 37955, 38237 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47278", "query_cov": 1.0, "subject_cov": 0.4894894894894895, "identity": 1.0, "score": 635.0, "evalue": 1.68e-224, "gene_start": 1, "gene_end": 326, "reference_start": 1, "reference_end": 326, "reference_sequence": "MKTIHKLFLGLCLPATLGPLLGIVVTNTDQSIKFTSKSNSINKNNQNKELALLRDNLMNEAKVDEPLSFEKRFENFKNKYSDIHSLNNSVFSLHDVYDLLGGFKQSLTTFFDEVIAQQQKIKDADKIFPSTKDNPPKEENPNVLDTLANYQGAGFFPSLGKNGFNLPEAVFQNFTDFRINDYKIKNFNVDLVSENDIIKHDKVRYAFEVKFNIALVLSINKSNVDFDFDFILKTDNFSDIENFNEIFNRKPALQFRFYTKINVHKLSFNGSDSTYIANILLQDQFNLLEIDLNKSIYALDLENAKERFDKEFVQPLYQKRREAKLAWEEEQRRIAEEQRRQEEERARILKELKEKAEKDKRVKEAQNNLQKALGNLDTFFNFFSSGQDRVLLGFDPNKYNVQTREGLFKALQISYSNFKTWTFYISLLGWKEGSVKLLKKPIWNALRDDKAFQYAFGLGPNTSEQQLGRVTLPGYGYEGIRMSDWLRWALGYYTSFTLSPPKNVEANLIGDANDKKHIWISPHTFKLNREYGDGERFKGKAYRFKLSISFELEGHLTAHWWTIAFRGSIPGSWSGKLRVTHEFDGDVPYYRLHTTPPQYRLTDDMKLLFVPHSIQRVTAVGNESINGLLRSQNLHNLERQSYEATAPIDLISYMLYAISDKKPPQK" }, "paralog": false, "identity": 0.9929742388758782, "score": 820.0, "evalue": 6.86e-300, "description": "Internal stop codon at 37955, 38237. Nonsense mutation around 37955, 38237.", "psc": { "uniref90_id": "UniRef90_P47278", "product": "Uncharacterized protein MG032", "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47278", "UniRef:UniRef50_P75078" ] }, "pscc": { "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078" ], "product": "Uncharacterized protein MPN_036" } }, "id": "KKNAAMEMLJ_108", "locus": "GCA000027325_00068" }, { "type": "cds", "sequence": "contig_1", "start": 38312, "stop": 38440, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAGATGATAAAGCATTTCAATATGCTTTTGGTTTAGGTCCAAATACTTCTGAACAACAACTTGGTAGAGTAACCCTACCTGGTTATGGTTATGAAGGAATTAGAATGAGTGATTGGTTGAGGTGA", "aa": "MRDDKAFQYAFGLGPNTSEQQLGRVTLPGYGYEGIRMSDWLR", "aa_hexdigest": "e72461b6d06e8627bac519a5b66a847d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4825.3553, "isoelectric_point": 6.0008562088012685 }, "id": "KKNAAMEMLJ_109", "locus": "GCA000027325_00069" }, { "type": "cds", "sequence": "contig_1", "start": 38735, "stop": 38977, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTTACTATAGATTACATACAACTCCACCACAATATCGTTTAACTGATGATATGAAATTATTGTTTGTTCCGCACAGTATCCAAAGGGTAACTGCAGTTGGTAATGAAAGCATTAATGGTCTTCTCAGATCACAAAACCTTCATAACTTGGAACGTCAATCATATGAAGCGACTGCTCCTATTGATTTAATATCATATATGCTTTATGCAATTAGTGATAAAAAACCACCTCAAAAATAA", "aa": "MPYYRLHTTPPQYRLTDDMKLLFVPHSIQRVTAVGNESINGLLRSQNLHNLERQSYEATAPIDLISYMLYAISDKKPPQK", "aa_hexdigest": "553ab238800d5e6fbb73df9d9f67e94f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9288.560799999996, "isoelectric_point": 9.048605155944824 }, "id": "KKNAAMEMLJ_110", "locus": "GCA000027325_00070" }, { "type": "cds", "sequence": "contig_1", "start": 39538, "stop": 39690, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATTGTACAAGTCCTAGTGTATTTAACATTACTAGGAATTTTCTAACTGAATTTATTGCAACTTTAATATTGATAGGTGGAGTTGTTGCTGCTAGTCACTTTCTTCATAACAACCCAAACTCTGTTCCTCCTGGATTTATGGGGCTTTGA", "aa": "MHCTSPSVFNITRNFLTEFIATLILIGGVVAASHFLHNNPNSVPPGFMGL", "aa_hexdigest": "53c8cee9ccee2d4afc287eae9f50904a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5384.216699999999, "isoelectric_point": 6.804616355895996 }, "id": "KKNAAMEMLJ_111", "locus": "GCA000027325_00071" }, { "type": "cds", "sequence": "contig_1", "start": 39691, "stop": 39813, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGCTGGGATTATTATTGCTTTTGGTGGCGCTACAGGCTCCGCAATTAATCCTGCAAGGGATTTGGGAACTAGAATTGTGTTTCAATTAACTCCAATTAAAAATAAGGATGCGAATTGA", "aa": "MVAGIIIAFGGATGSAINPARDLGTRIVFQLTPIKNKDAN", "aa_hexdigest": "e7c296cb1df5ea928decea3c3b59e0b5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4112.752800000002, "isoelectric_point": 9.984299278259279 }, "id": "KKNAAMEMLJ_112", "locus": "GCA000027325_00072" }, { "type": "cds", "sequence": "contig_1", "start": 40542, "stop": 41069, "strand": "+", "frame": 3, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "COG:COG0124", "COG:J", "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "SO:0001217", "UniRef:UniRef50_P47281", "UniRef:UniRef90_P47281" ], "nt": "ATGAACTTCTTGCAAAAACCAAGGGGAGTTAAAGATTGGTTTGGTGATGAATTAGTTTATTTTAATTGGATTGTTAAAAAAATAAGATCTTTAGCATTTAATTGGGGTTTTAGTGAAGTTAAAACTCCGTTGTTTGAAAATGCACAACTTTTTCAAAGATCTAATGCTAATGCTGATATTGTTCAAAAAGAACTATACCAGTTTTTTGATAAATCTCAAAGAGAATTAGCTTTAAGACCTGAAGCTACTACACCAATAGTAAGACTTGCTTGTGAAAACAAATTAATGCAAGAAGCAAATTTTCCCTTAAAGTTATTTTGCATTGGTTCAATGTATCGTTATGAACGTCCACAAAACAATAGGTTTCGTGAACATTGGCAATTTAGTTGCGAAGTATTTGGTTTTTCCAACCTGTTTATCTTTTTAGATACACTTTTGTTTGCTAACTCTTTGCTTGAAGCACTTGGAATTACTGGATATGTGCTTAAAATTAATAATCTTGCTAACTTTGAAACACTTAGTAAGTGA", "aa": "MNFLQKPRGVKDWFGDELVYFNWIVKKIRSLAFNWGFSEVKTPLFENAQLFQRSNANADIVQKELYQFFDKSQRELALRPEATTPIVRLACENKLMQEANFPLKLFCIGSMYRYERPQNNRFREHWQFSCEVFGFSNLFIFLDTLLFANSLLEALGITGYVLKINNLANFETLSK", "aa_hexdigest": "d5e7ed0c8f541ae38241a30e55e7c24a", "start_type": "ATG", "rbs_motif": null, "genes": [ "hisS" ], "truncated": "3-prime", "pseudogene": { "start": 40542, "stop": 41372, "observations": { "mutation": [ 41067, 41295 ], "stop": [ 41067, 41295 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47281", "query_cov": 1.0, "subject_cov": 0.4227053140096618, "identity": 1.0, "score": 357.0, "evalue": 3.24e-121, "gene_start": 1, "gene_end": 175, "reference_start": 1, "reference_end": 175, "reference_sequence": "MNFLQKPRGVKDWFGDELVYFNWIVKKIRSLAFNWGFSEVKTPLFENAQLFQRSNANADIVQKELYQFFDKSQRELALRPEATTPIVRLACENKLMQEANFPLKLFCIGSMYRYERPQNNRFREHWQFSCEVFGFSNLFIFLDTLLFANSLLEALGITGYVLKINNLANFETLSKWNKALKDYLTPYKLELTELSQKRLEKNPLRILDDKIDQKKSFVKNAPKITDFLDASAKQDSELLKTQLKKHNISFEWTDNLVRGLDYYTGFVFEYVKNQDTILAGGVYDNLVEELSSNPTPALGFACGIERLINCLEIDKKAFILNTKPKQMLVICLFEEALEELVWLAKLWREYNQVTIYPKVIKVDNGIRLANRLGYTFIGIVGKTDFDKKAITIKNLVSKQQTIYTWNELGERNVF" }, "paralog": false, "identity": 0.9927536231884058, "score": 552.0, "evalue": 1.46e-200, "description": "Internal stop codon at 41067, 41295. Nonsense mutation around 41067, 41295.", "psc": { "uniref90_id": "UniRef90_P47281", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_P47281", "cog_id": "COG0124", "cog_category": "J", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47281", "EC:6.1.1.21", "UniRef:UniRef50_P47281", "COG:COG0124", "COG:J", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_P47281", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47281" ], "product": "Histidine--tRNA ligase" } }, "id": "KKNAAMEMLJ_113", "locus": "GCA000027325_00073" }, { "type": "cds", "sequence": "contig_1", "start": 41151, "stop": 41297, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAATTTTAGATGACAAGATAGATCAAAAAAAATCATTTGTTAAAAATGCTCCTAAAATTACTGATTTTTTAGATGCAAGTGCAAAACAAGATTCAGAATTGTTAAAAACACAACTAAAAAAACACAATATTAGTTTTGAATGA", "aa": "MRILDDKIDQKKSFVKNAPKITDFLDASAKQDSELLKTQLKKHNISFE", "aa_hexdigest": "e9f398681deccbdb36eac70c9c1b963f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5577.368899999999, "isoelectric_point": 9.10056667327881 }, "id": "KKNAAMEMLJ_114", "locus": "GCA000027325_00074" }, { "type": "cds", "sequence": "contig_1", "start": 41319, "stop": 41567, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "TTGGATTACTATACTGGATTTGTGTTTGAATATGTAAAAAATCAAGACACAATTTTAGCAGGTGGAGTTTATGATAACTTAGTTGAAGAATTAAGTAGTAATCCAACTCCCGCATTAGGTTTTGCTTGTGGAATTGAACGGTTAATTAACTGTTTAGAAATTGATAAAAAAGCATTTATTTTGAATACTAAACCAAAGCAGATGTTAGTAATTTGCTTATTTGAAGAAGCGCTTGAAGAATTGGTTTGA", "aa": "MDYYTGFVFEYVKNQDTILAGGVYDNLVEELSSNPTPALGFACGIERLINCLEIDKKAFILNTKPKQMLVICLFEEALEELV", "aa_hexdigest": "5b2c62c773554382779f3964497e95a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 49, "aa_cov": 0.573170731707317, "hmm_cov": 0.9795918367346939, "evalue": 2.6610943129868966e-10, "score": 35.041114807128906, "start": 2, "stop": 48 } ], "seq_stats": { "molecular_weight": 9253.618999999997, "isoelectric_point": 4.2809652328491214 }, "id": "KKNAAMEMLJ_115", "locus": "GCA000027325_00075" }, { "type": "cds", "sequence": "contig_1", "start": 42352, "stop": 42888, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATTGCAGGAGTTGAAAAATATTTTCAAATTGCAAGGGTCTATCGTGATGAAGATAGCAGAAAAGATCGTCAACCAGAACACACACAAATTGATTTCGAGATCTCTTTTTGTAACCAAAAAATGATTATGAATCTAGTTGAAAAACTCTTTTTTAGTGTTTTCTTAGATGTTTTTCAAATCAAAATAAAAAAGACTTTTCCTGTTTTTAAATTTTCAGAACTTTTTGAAAGATTTGGTAGCGATAAACCAGATTTACGTTATGGTTTTGAAATAAAAGATTTCACCTCGCTTTTTCAAGATCATCAGAATCAGTTCACTAAATTAATTGAAGCAAAAGGCATTATTGGTGGTATTGAACTTACTAATATTGAGTTAAGTACAGACAAAATTAAAGCATTAAGAAAAATTGCTAAGGACCATGATGTGAGTTTAGAAGTTCATAATAAAAATAATTCAACATTAAAAACTTCAATTAAATGTGATGAAAAAAACACTCTTCTGTTAGTAGCAAATAAATCTAAAAAGAAGGCATGA", "aa": "MIAGVEKYFQIARVYRDEDSRKDRQPEHTQIDFEISFCNQKMIMNLVEKLFFSVFLDVFQIKIKKTFPVFKFSELFERFGSDKPDLRYGFEIKDFTSLFQDHQNQFTKLIEAKGIIGGIELTNIELSTDKIKALRKIAKDHDVSLEVHNKNNSTLKTSIKCDEKNTLLLVANKSKKKA", "aa_hexdigest": "02319725e1814c0aaee1acb097fea956", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 115, "aa_cov": 0.6179775280898876, "hmm_cov": 0.8608695652173913, "evalue": 2.6891916080768653e-30, "score": 100.53925323486328, "start": 1, "stop": 110 } ], "seq_stats": { "molecular_weight": 20805.805700000023, "isoelectric_point": 9.081935310363772 }, "id": "KKNAAMEMLJ_116", "locus": "GCA000027325_00076" }, { "type": "cds", "sequence": "contig_1", "start": 43180, "stop": 43404, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGAATTCTTTGAACATTGACCCAAATAAGTTTGCTTTTCTTCTAGAAGCTTATCAATATGGTGCTCCTGTTCATGGTGGAATGGGACTAGGTATTGATCGTTTAATGATGATTCTTAATCAAACTGATAACATCAGAGAAGTAATCGCTTTTCCTAAGAATAATCATGGTATTGAAGTCCATACAAACGCTCCTGATAAAATTGACAAAGAGGAGGTTAAATGA", "aa": "MNSLNIDPNKFAFLLEAYQYGAPVHGGMGLGIDRLMMILNQTDNIREVIAFPKNNHGIEVHTNAPDKIDKEEVK", "aa_hexdigest": "c282332fe940318a3d13f7bf6d6cb25e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 52, "aa_cov": 0.6486486486486487, "hmm_cov": 1.0, "evalue": 7.289706454466616e-20, "score": 66.27344512939453, "start": 7, "stop": 54 } ], "seq_stats": { "molecular_weight": 8308.420099999996, "isoelectric_point": 5.430363273620604 }, "id": "KKNAAMEMLJ_117", "locus": "GCA000027325_00077" }, { "type": "cds", "sequence": "contig_1", "start": 43401, "stop": 43565, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGATAAAAGAACTAGTGAAATAGAGTTTCACTTAAAGAATCTTTTGGCTTGTGATGCATATAAACTTTCACACCGTTTAATGTATCCACAAGATACACAAAACCTTTATAGTATGTTAACTGCAAGAGGTGTTTATGGTGATTTTAAGGAGTTTGTTTGA", "aa": "MMDKRTSEIEFHLKNLLACDAYKLSHRLMYPQDTQNLYSMLTARGVYGDFKEFV", "aa_hexdigest": "3aea623054ed15a2c98367d5a220241b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6420.354400000002, "isoelectric_point": 6.702988243103028 }, "id": "KKNAAMEMLJ_118", "locus": "GCA000027325_00078" }, { "type": "cds", "sequence": "contig_1", "start": 43593, "stop": 44258, "strand": "+", "frame": 3, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:2.4.2.12", "EC:6.3.4.21", "GO:0004514", "GO:0009435", "GO:0047280", "SO:0001217", "UniRef:UniRef50_P75067", "UniRef:UniRef90_P47283" ], "nt": "TTGAATGTATTTAATGGTTTTGTAAACAGTGTAATTGAAGTTAAAAAAAACAAATTGCTAGCTGCAGCATTGACAGATAAATTAGTTAGTGTTTTTAATGATCATGAATTGGCTAATGAATTCACACAACACATCTGTCATTTAGCTAGTTTCTTAGAGAAAAATAAAAAAATGCCGTTAGTTGCAAAGATCCATGAAAGTGATCAATCATTACCATTTAGAACTCCTTTAATAACTATAGAAGGAGTTGAAAATATTCCAAACAACTTTGTATGGTTAGTTAATTACTTTGAAACTGTACTTCTAGAAAACATTTGGTTGTTTCAAACTGCTTCTACAGTTGCTAAAAGAATTAAATCTTTACTTGAAAAATATGCTAAAGAAACCGCAGATGAAACAAGTTTTATTAATTTTCAATGCCACGACTTTAGTATGCGGGGCATGAGTAGTTTGCAAAGTGCTTTGTATGTTGCTAGAGCACACTTGCAATACTTTACTGGAAGTGACACGATCTTAGGTGGGGATAATTCTCGTTCAATTTTAGCTTCTGAACATTCAGTGATGTGCGCAGATGGTAGTAAACATGAATTGAAAACTTTTCAACGTTTATTGGAAAAGTTTAAAGATAAAAAACTTTCTTTAGTGATTGATTCTTATGACATGTGA", "aa": "MNVFNGFVNSVIEVKKNKLLAAALTDKLVSVFNDHELANEFTQHICHLASFLEKNKKMPLVAKIHESDQSLPFRTPLITIEGVENIPNNFVWLVNYFETVLLENIWLFQTASTVAKRIKSLLEKYAKETADETSFINFQCHDFSMRGMSSLQSALYVARAHLQYFTGSDTILGGDNSRSILASEHSVMCADGSKHELKTFQRLLEKFKDKKLSLVIDSYDM", "aa_hexdigest": "6c1f5a2bd9103dc1729ee323db100032", "start_type": "TTG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 43401, "stop": 44561, "observations": { "mutation": [ 43563, 44256, 44397 ], "stop": [ 43563, 44256, 44397 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47283", "query_cov": 1.0, "subject_cov": 0.4911111111111111, "identity": 0.995, "score": 436.0, "evalue": 3.66e-151, "gene_start": 1, "gene_end": 221, "reference_start": 65, "reference_end": 285, "reference_sequence": "MMDKRTSEIEFHLKNLLACDAYKLSHRLMYPQDTQNLYSMLTARGVYGDFKEFVWNHDFAKEILLNVFNGFVNSVIEVKKNKLLAAALTDKLVSVFNDHELANEFTQHICHLASFLEKNKKMPLVAKIHESDQSLPFRTPLITIEGVENIPNNFVWLVNYFETVLLENIWLFQTASTVAKRIKSLLEKYAKETADETSFINFQCHDFSMRGMSSLQSALYVARAHLQYFTGSDTILGGDNSRSILASEHSVMCADGSKHELKTFQRLLEKFKDKKLSLVIDSYDMWNVLDNIIPRLKNLILMRGATLYLRADSGNYQTLICNPNYKKQDKSTWAMIDYLDHHFSSTINKKGYKVLNKKIGIIYGDGITYQKIEWILNCLKNHGYCSSNIIFGVGSSTYQNLNRDTLGFVYKLTAIKRNNRWIGVKKTPITDLSKSSKGGRYKTKRLITVY" }, "paralog": false, "identity": 0.9922279792746114, "score": 768.0, "evalue": 1.34e-284, "description": "Internal stop codon at 43563, 44256, 44397. Nonsense mutation around 43563, 44256, 44397.", "psc": { "uniref90_id": "UniRef90_P47283", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "2.4.2.12", "6.3.4.21" ], "uniref50_id": "UniRef50_P75067", "cog_id": "COG1488", "cog_category": "H", "go_ids": [ "GO:0004514", "GO:0009435", "GO:0047280" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47283", "EC:2.4.2.12", "EC:6.3.4.21", "UniRef:UniRef50_P75067", "COG:COG1488", "COG:H", "GO:0004514", "GO:0009435", "GO:0047280" ] }, "pscc": { "uniref50_id": "UniRef50_P75067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75067" ], "product": "Nicotinamide phosphoribosyltransferase" } }, "id": "KKNAAMEMLJ_119", "locus": "GCA000027325_00079" }, { "type": "cds", "sequence": "contig_1", "start": 44304, "stop": 44399, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGGTGCTACGCTTTATTTGCGTGCTGATTCTGGTAATTATCAAACTCTTATTTGCAATCCTAATTACAAAAAGCAAGATAAAAGTACATGA", "aa": "MRGATLYLRADSGNYQTLICNPNYKKQDKST", "aa_hexdigest": "38cc5b37f7e62f36e580fa085a08b84f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3550.973400000001, "isoelectric_point": 9.428839683532715 }, "id": "KKNAAMEMLJ_120", "locus": "GCA000027325_00080" }, { "type": "cds", "sequence": "contig_1", "start": 44403, "stop": 44753, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGATTACTTAGATCATCATTTTAGTTCAACTATAAATAAAAAAGGTTATAAGGTTTTAAACAAGAAAATTGGCATTATTTATGGTGATGGAATCACCTATCAAAAGATAGAATGGATCTTAAATTGTTTAAAAAACCATGGTTATTGTTCTTCAAACATTATTTTTGGAGTTGGTAGTAGCACTTATCAAAATTTAAACCGTGATACTTTAGGTTTTGTATACAAATTGACTGCTATTAAAAGAAATAATAGATGGATAGGCGTTAAAAAAACTCCCATAACTGATCTATCTAAAAGTTCAAAAGGCGGTAGATATAAAACAAAGCGATTAATTACAGTTTATTAA", "aa": "MIDYLDHHFSSTINKKGYKVLNKKIGIIYGDGITYQKIEWILNCLKNHGYCSSNIIFGVGSSTYQNLNRDTLGFVYKLTAIKRNNRWIGVKKTPITDLSKSSKGGRYKTKRLITVY", "aa_hexdigest": "ae167cd7ff186095ed04defb24c6fbfc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13328.328799999977, "isoelectric_point": 9.93942928314209 }, "id": "KKNAAMEMLJ_121", "locus": "GCA000027325_00081" }, { "type": "cds", "sequence": "contig_1", "start": 44891, "stop": 45070, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02782.22" ], "nt": "ATGGCAAGTGATCTAGGCTATAAGATTACTAGCATTAAAGCTGATGGGGGGATTGTTAAATCAAACTATTTAATGCAGTTTCAAGCTGATATTGCAGATGTAATTGTTTCTATCCCTAAAAATAAAGAAACCACTGCAGTTGGTGTTTGTTTTTTAGCTGGACTTGCTTGTGGATTTTGA", "aa": "MASDLGYKITSIKADGGIVKSNYLMQFQADIADVIVSIPKNKETTAVGVCFLAGLACGF", "aa_hexdigest": "3f2c8f6f49e1429fbcee68c249e0bd6e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02782.22", "name": "FGGY_C", "length": 54, "aa_cov": 0.9152542372881356, "hmm_cov": 1.0, "evalue": 7.243832261615863e-13, "score": 43.813819885253906, "start": 3, "stop": 56 } ], "seq_stats": { "molecular_weight": 6185.1698, "isoelectric_point": 5.950326347351075 }, "id": "KKNAAMEMLJ_122", "locus": "GCA000027325_00082" }, { "type": "cds", "sequence": "contig_1", "start": 45188, "stop": 45511, "strand": "-", "frame": 2, "gene": null, "product": "FGGY-family carbohydrate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D3F047" ], "nt": "TTGTTTGGTCAACTCTGTACTGAACCTGGAATGGTAAAAAATACCTATGGTACTGGATGTTTTGTACTCATGAACATTGGTGATAAACCAACACTCTCAAAGCACAATCTGCTCACAACAGTAGCATGGCAACTAGAAAATCATCCACCTGTATATGCATTGGAAGGTAGTGTGTTTGTAGCGGGTGCGGCTATAAAATGGTTAAGGGATGCATTAAAAATTATCTATTCAGAAAAGGAAAGTGATTTTTATGCAGAACTTGCAAAAGAAAATGAACAAAACCTAGTTTTTGTACCAGCTTTCAGTGGACTTGGAGCTCCTTGA", "aa": "MFGQLCTEPGMVKNTYGTGCFVLMNIGDKPTLSKHNLLTTVAWQLENHPPVYALEGSVFVAGAAIKWLRDALKIIYSEKESDFYAELAKENEQNLVFVPAFSGLGAP", "aa_hexdigest": "b2b5cbb17958490f9a654d213f18af7c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027D3F047", "query_cov": 1.0, "subject_cov": 0.9304347826086956, "identity": 0.523, "score": 115.0, "evalue": 3.92e-31, "valid": false, "product": "FGGY-family carbohydrate kinase", "uniref50_id": "UniRef50_UPI0027D3F047" }, "pscc": { "uniref50_id": "UniRef50_UPI0027D3F047", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D3F047" ], "product": "FGGY-family carbohydrate kinase" }, "genes": [], "id": "KKNAAMEMLJ_123", "locus": "GCA000027325_00083" }, { "type": "cds", "sequence": "contig_1", "start": 45606, "stop": 45866, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACTCAGAACTTTAGGTAAGATTGAAACTGGTACTTCAAATAAATCACATAACTCTTTGGATCACTCCATTTTGACAATGTCAAATAAAAGAGTTCTTGAAGCATTTGAAACATCTGTAACATGCATTTTTCCATTAGTTAGTTTTCAGATTAATCAGCTATCAATGGTGCCAAATAACAACTTTTTTTGCTCCATTAGTTTCTTTGCTAAAGGAACATTTTTTAAGATTCAAGCTATCTTAGTAGCACTAAAATAG", "aa": "MELRTLGKIETGTSNKSHNSLDHSILTMSNKRVLEAFETSVTCIFPLVSFQINQLSMVPNNNFFCSISFFAKGTFFKIQAILVALK", "aa_hexdigest": "36179cddd9d56581e09050ccd2654431", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9647.159099999992, "isoelectric_point": 9.18437557220459 }, "id": "KKNAAMEMLJ_124", "locus": "GCA000027325_00084" }, { "type": "cds", "sequence": "contig_1", "start": 46004, "stop": 46093, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGTGCTAAAAATAAAGCACAAATCAAATCTCATGAAGTGATTGCAGTTGGTATTACCAATCAAAGAGAAACAATAGTTTTATGA", "aa": "MQSAKNKAQIKSHEVIAVGITNQRETIVL", "aa_hexdigest": "159464c92f69e0a07a6228989c9a85cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3207.7024, "isoelectric_point": 9.697157096862792 }, "id": "KKNAAMEMLJ_125", "locus": "GCA000027325_00085" }, { "type": "cds", "sequence": "contig_1", "start": 46139, "stop": 46276, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCTAAAAAAACAATACATTATTGCCTTAGATGAAGGTACTAGTTCTTGTCGATCAATTGTTTTTGATCACAATCTTAACCAAATAGCAATAGCACAAAACGAATTTAACACTTTTTTTCCTAATAGTGGTTGA", "aa": "MDLKKQYIIALDEGTSSCRSIVFDHNLNQIAIAQNEFNTFFPNSG", "aa_hexdigest": "355f63ae6572bcf6c847359ea0119fdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5091.643299999999, "isoelectric_point": 4.8263353347778315 }, "id": "KKNAAMEMLJ_126", "locus": "GCA000027325_00086" }, { "type": "cds", "sequence": "contig_1", "start": 46297, "stop": 46671, "strand": "-", "frame": 3, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "nt": "ATGGTACCTACTATCCATGGTAAGGGAGTAGTTGTTGCTGAGATGTTAGATGGTAATATCCTTGTGGGACCTAATGCAGTTGAGGGAATTGAAAAAAACAAAACTCGTTCCATAGATTTAGATTCAATTAACCAAATTAAAACTATTGGTAAAAAAATGGTTCCTTCATTGCAATTTGAAAATAGTATTTATAGTTTTGCAGGTTCAAGAGCTATTGATATTGAAACAAATGACTTTGTTATTAGAACAGCAAAAAGCAATCCTAACTTTATCATCCTTGGTGGCATGAAATCCCCTGGTTTAACATCAAGTCCTGCTATTGCTAAGAGAGCAGTTGAACTCCTTAATTTAAAGTTAAAAAAGAAGATAAATTGA", "aa": "MVPTIHGKGVVVAEMLDGNILVGPNAVEGIEKNKTRSIDLDSINQIKTIGKKMVPSLQFENSIYSFAGSRAIDIETNDFVIRTAKSNPNFIILGGMKSPGLTSSPAIAKRAVELLNLKLKKKIN", "aa_hexdigest": "e7198bb38adc51876e45e2edfef62437", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF40EBC", "query_cov": 0.9919354838709677, "subject_cov": 0.8601398601398601, "identity": 0.561, "score": 130.0, "evalue": 1.02e-36, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A449B2Q2" }, "pscc": { "uniref50_id": "UniRef50_A0A449B2Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "product": "Glycerol-3-phosphate dehydrogenase" }, "genes": [], "id": "KKNAAMEMLJ_127", "locus": "GCA000027325_00087" }, { "type": "cds", "sequence": "contig_1", "start": 46975, "stop": 47109, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAAGAAAGGGGAATTAAAAACTCAATTCCTGTTGAAAACATTCAAATATTAGATCAACAACAAACTTTATTGCAAGAACCGTTTATCAATCCAAATGTAGTAGCTAGTTTAAAAGTTGAAGGTAGTTGA", "aa": "MLKERGIKNSIPVENIQILDQQQTLLQEPFINPNVVASLKVEGS", "aa_hexdigest": "e895ac8fa1d30d23f4d04a1af7da3792", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4932.6494, "isoelectric_point": 5.002479362487793 }, "id": "KKNAAMEMLJ_128", "locus": "GCA000027325_00088" }, { "type": "cds", "sequence": "contig_1", "start": 47203, "stop": 47421, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01266.30", "PFAM:PF13450.12", "PFAM:PF00070.33" ], "nt": "ATGCAAACAATTGACGTTTTAATTGTGGGTGGGGGTGTCATCGGTACTAGTTGTGCATATGAACTCAGCCAATATAAATTAAAAGTTGCACTTTTAGAAAAAAATGCTTTTTTGGGTTGTGAAACTTCGCAGGCTAACAGTGGTGTAATCCATTCAGGAATTGATCCTAATCCTAATAAGCTAACTGCCAAATACAACATCTTAGGGAGAAAAATCTGA", "aa": "MQTIDVLIVGGGVIGTSCAYELSQYKLKVALLEKNAFLGCETSQANSGVIHSGIDPNPNKLTAKYNILGRKI", "aa_hexdigest": "c6e04e1b2c34c60998973590ae6100c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01266.30", "name": "DAO", "length": 60, "aa_cov": 0.8055555555555556, "hmm_cov": 1.0, "evalue": 3.4273896220337263e-20, "score": 67.88536834716797, "start": 5, "stop": 62 }, { "id": "PF13450.12", "name": "NAD_binding_8", "length": 36, "aa_cov": 0.5, "hmm_cov": 1.0, "evalue": 1.6106993522398806e-09, "score": 33.05045700073242, "start": 8, "stop": 43 }, { "id": "PF00070.33", "name": "Pyr_redox", "length": 31, "aa_cov": 0.4305555555555556, "hmm_cov": 1.0, "evalue": 1.4233132263657974e-06, "score": 23.84930419921875, "start": 6, "stop": 36 } ], "seq_stats": { "molecular_weight": 7665.8180999999995, "isoelectric_point": 8.661279106140139 }, "id": "KKNAAMEMLJ_129", "locus": "GCA000027325_00089" }, { "type": "cds", "sequence": "contig_1", "start": 47580, "stop": 47855, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAGCTATTATTTGGTAAGTTAGTTTTTAAAAAGAGCTTGTTTTTACTGAGTGGTATGAGCAGTTTAGCAGTTTTTTTAACTGCATGTGGGGCTACCAAAATATTTGATTCCTCTGTACAGCTATTAGTGTCAGATAACTTTTCCACACTTGCTGATAAATCATTTTCACAAATGTCCTATGAAGGAATCAGGAGCTTTTTTAAAAAAAGTAAGGGTGTTGATTTACCTGAAGCAGATAGTTCACAATTACAAGAAGGCAATGGCTTGTGA", "aa": "MGKLLFGKLVFKKSLFLLSGMSSLAVFLTACGATKIFDSSVQLLVSDNFSTLADKSFSQMSYEGIRSFFKKSKGVDLPEADSSQLQEGNGL", "aa_hexdigest": "8af69b5d4bd6c278f255f2095abea66e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9840.283899999993, "isoelectric_point": 8.818388557434083 }, "id": "KKNAAMEMLJ_130", "locus": "GCA000027325_00090" }, { "type": "cds", "sequence": "contig_1", "start": 48333, "stop": 48929, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTATAAACATTTAAGCGATGCACAAGATTCATCTGCACAAGTGACCACTTCTAAACAAACTGTTTTAAAACAACTTCAAGTTGCAAATGGTGAAAAAAGGATTAAAAAGATTAAATGGATTTCACCAAAACAAGGAAGTGATGGAGAAACTATAAACATTCAAGATCACCAATCAGGTTCTTTTTCAGATACTGAACCTAGAGCAATAACAATAGCTAATAATTTAATTGATAAAGGAGTTAATGCTATCATTCCTATTGCTGGACCACAAACGAATTTAGTGGTTACTCAAATTGCTAGAAGACAAGCCCATACTGCAGTTATTGGCGTTGATAGTGCACAGGAATTGCTAGATATTAATATTGATGCTCCAAATAAAGATAAGTTAAAAATGGGGAATAAAAAGATTATTCCCTTCTCTTCTATTAAGGCTTTGGATGTTGCTGTTGAAAGTATCTTATCAACATTAGAAAAAGGTTCCAGCCAAAATGGTTATCAAGGCTTTGGATATAACAACATAGGTACAGTGAAAAACAACTCTGTTGGGGTTAGTGAAGCAGGTTATGAATTTTTAATAGATCCTGTTTTTTGA", "aa": "MIYKHLSDAQDSSAQVTTSKQTVLKQLQVANGEKRIKKIKWISPKQGSDGETINIQDHQSGSFSDTEPRAITIANNLIDKGVNAIIPIAGPQTNLVVTQIARRQAHTAVIGVDSAQELLDINIDAPNKDKLKMGNKKIIPFSSIKALDVAVESILSTLEKGSSQNGYQGFGYNNIGTVKNNSVGVSEAGYEFLIDPVF", "aa_hexdigest": "968034ad7e778f04dbd55bab107cfe01", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21391.945899999984, "isoelectric_point": 7.880244636535644 }, "id": "KKNAAMEMLJ_131", "locus": "GCA000027325_00091" }, { "type": "cds", "sequence": "contig_1", "start": 48945, "stop": 49190, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTATGTCTTTATCAGCAAGTCTAAAAGCTAATGCAGCATCTTCATCAGATAATAAGAAAAAATTATCAGAAGTTGCTACTAAGAAAAATGAAAACGGTTCGACAAAAAATGGTAGTAATGACATCATTGACAAATATGCCAAACTCTTAACAAAATCTAGTTCTTCAACTAGTATGAGAAACGGTAGTTCAGATAGCAATCAACAGAATTTTAAAACAACAGATAATGATGGTGATTGA", "aa": "MQAMSLSASLKANAASSSDNKKKLSEVATKKNENGSTKNGSNDIIDKYAKLLTKSSSSTSMRNGSSDSNQQNFKTTDNDGD", "aa_hexdigest": "be67ae5483e0687045642b172e5e6686", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8581.206899999996, "isoelectric_point": 9.301836967468262 }, "id": "KKNAAMEMLJ_132", "locus": "GCA000027325_00092" }, { "type": "cds", "sequence": "contig_1", "start": 49376, "stop": 49642, "strand": "+", "frame": 2, "gene": "ptsH", "product": "Phosphocarrier protein HPr", "db_xrefs": [ "COG:COG1925", "COG:TG", "GO:0005737", "GO:0009401", "RefSeq:WP_010869302.1", "SO:0001217", "UniParc:UPI00001328F4", "UniRef:UniRef100_P47287", "UniRef:UniRef50_P75061", "UniRef:UniRef90_P47287" ], "nt": "ATGAAAAAATTTCAAGCAGTTATTAAAGACCCAGTGGGAATTCACGCACGTCCTGCTTCTATCCTTGCAAGTGAGGCTAGTAAGTTTAAATCTGAACTTAAACTGGTAGCTCCAAGTGGTGTTGAAGGTAATATTAAATCAATTATTAACTTAATGTCTTTAGGAATTAGACATAATGACAACATTACTATCAAAGCAGATGGAGCTGATGAAGAAGAAGCTTTAGCAGCTATTAAAGCTTGTCTTGAAAAAAATAAAGTTATCTAA", "aa": "MKKFQAVIKDPVGIHARPASILASEASKFKSELKLVAPSGVEGNIKSIINLMSLGIRHNDNITIKADGADEEEALAAIKACLEKNKVI", "aa_hexdigest": "e0b02b7b8a2b84a6d8cee18d7f134960", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001328F4", "ncbi_nrp_id": "WP_010869302.1", "uniref100_id": "UniRef100_P47287", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001328F4", "RefSeq:WP_010869302.1", "UniRef:UniRef100_P47287" ] }, "ips": { "uniref100_id": "UniRef100_P47287", "uniref90_id": "UniRef90_P47287" }, "psc": { "uniref90_id": "UniRef90_P47287", "gene": "ptsH", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_P75061", "cog_id": "COG1925", "cog_category": "TG", "go_ids": [ "GO:0005737", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75061" ], "product": "Phosphocarrier protein HPr" }, "genes": [ "ptsH" ], "id": "KKNAAMEMLJ_133", "locus": "GCA000027325_00093" }, { "type": "cds", "sequence": "contig_1", "start": 49840, "stop": 50163, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920RY59" ], "nt": "TTGAACGAACATTCTTTAATTGAAATTGAAGGTTTGAACAAGACCTTTGATGATGGTTATGTTTCTATAAGAGACATTAGCCTAAATATTAAAAAAGGCGAATTTATTACTATTTTAGGCCCTTCTGGTTGTGGTAAAACTACCCTGTTGAGGTTATTAGCTGGATTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTATTGACATTAAAGACATGGCAATCCATAAGCGTCCTTTTGCGACAGTTTTTCAAGACTATGCTTTATTTTCCCATCTAACTGTTTATAAAAACATTGCTTATGGTCTGAAGGTAATGTGA", "aa": "MNEHSLIEIEGLNKTFDDGYVSIRDISLNIKKGEFITILGPSGCGKTTLLRLLAGFEDPTYGKIKVNGIDIKDMAIHKRPFATVFQDYALFSHLTVYKNIAYGLKVM", "aa_hexdigest": "afb91b292ce699a39b0de5f268b09fee", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920RY59", "query_cov": 0.9719626168224299, "subject_cov": 0.9541284403669725, "identity": 0.519, "score": 112.0, "evalue": 5.42e-30, "valid": false, "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_A0A920RY59" }, "pscc": { "uniref50_id": "UniRef50_A0A920RY59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920RY59" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "KKNAAMEMLJ_134", "locus": "GCA000027325_00094" }, { "type": "cds", "sequence": "contig_1", "start": 50572, "stop": 50724, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGCTGATATTAAAGCAACAAAGAACAAAGAACAAATCCAAAAATTAACTAAAGAACTTCAAACCTTAAAACAAAAATACGCTAATAAAAAAGCAATTGACAAAGAGTATGACAAATTAGTTGTAGCTTACAATAAGAAAGACTATTGA", "aa": "MKADIKATKNKEQIQKLTKELQTLKQKYANKKAIDKEYDKLVVAYNKKDY", "aa_hexdigest": "18160e4ff5543dacd31e6a7f419d6c81", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5947.898900000004, "isoelectric_point": 9.699220085144042 }, "id": "KKNAAMEMLJ_135", "locus": "GCA000027325_00095" }, { "type": "cds", "sequence": "contig_1", "start": 50839, "stop": 51168, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "nt": "ATGGTTGGTTTAAAAGGTAAAGAAGATCGTTTGCCTGATGAATTATCAGGGGGAATGAAACAAAGAGTTGCTTTAGCACGTTCTTTAGTAGTAGAACCTGAAATTCTTTTATTAGATGAACCATTATCTGCACTTGATGCAAAGGTTAGAAAGAATTTACAAAAAGAATTACAACAGATTCATAAAAAAAGTGGATTGACTTTTATCTTAGTAACTCATGATCAAGAAGAGGCTTTAGTTTTATCAGATCGGATAGTGGTTATGAATGAGGGAAACATCTTACAAGTTGGTAATCCTGTTGATATTTATGACTCTCCTAAGACTGAATGA", "aa": "MVGLKGKEDRLPDELSGGMKQRVALARSLVVEPEILLLDEPLSALDAKVRKNLQKELQQIHKKSGLTFILVTHDQEEALVLSDRIVVMNEGNILQVGNPVDIYDSPKTE", "aa_hexdigest": "2ac62bb188addd48c6339b1c99c3a907", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00237D6FE8", "query_cov": 0.9908256880733946, "subject_cov": 0.8503937007874016, "identity": 0.574, "score": 124.0, "evalue": 1.36e-34, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00237D6FE8" }, "pscc": { "uniref50_id": "UniRef50_UPI00237D6FE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "KKNAAMEMLJ_136", "locus": "GCA000027325_00096" }, { "type": "cds", "sequence": "contig_1", "start": 51572, "stop": 51700, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATCTTCTTTATTATTCCAATGGCATGGATTATTGTTAGTGGATTACAAAGTGAAGATGGGGCTAGTATTAGTCAAAAATATGAACCACTTGTTAGTGGCTTAGGTTTTTTTAACAGTTTCTGA", "aa": "MTIFFIIPMAWIIVSGLQSEDGASISQKYEPLVSGLGFFNSF", "aa_hexdigest": "201ce317b84d1ed057cba098cbd0515d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4642.347599999999, "isoelectric_point": 4.136935234069824 }, "id": "KKNAAMEMLJ_137", "locus": "GCA000027325_00097" }, { "type": "cds", "sequence": "contig_1", "start": 51863, "stop": 52357, "strand": "+", "frame": 2, "gene": "potB", "product": "Spermidine/putrescine transport system permease PotB-like protein", "db_xrefs": [ "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47289", "UniRef:UniRef90_P47289" ], "nt": "TTGAAAACCCTACTTGATTTATTAATTGGACTTTCTTTAAACAGAGTTGGTGATAACAACTTAACTTTTGGTTCAGGATATACCTTACTTGGAACAATTTATCTGTTTACTCCTTTTATGTTTTTACCACTTTATAACCACTTCTGTGTTTTACCTAAAAACTTGTTGTTAGCTAGTCAAGATTTGGGTTATAACTGGATTTACAGCTTTGTGAAAGTAGTAATTCCTTTTTCTAAAACCGCAATGTTATCAGGAATTGCTTTAACTTTTTTCCCTGCTTTAACTTCAGTTGCAATTGCTCAGTTTTTAGATAACTCTAACCAAGCCGAAACCCTTGGTAACTACATATTTACCTTGGGTAATAATGGTTATGATAGTGCAATTGAAAGAGGCAGAGCTGCTGGAGCAATTATTATTGCTGCTTTAATTACTTTTGCAATTTACTTTACTGTTGTTTTTTTGCCTAAAATTGTCCGTATTGTTCATAACAAATGA", "aa": "MKTLLDLLIGLSLNRVGDNNLTFGSGYTLLGTIYLFTPFMFLPLYNHFCVLPKNLLLASQDLGYNWIYSFVKVVIPFSKTAMLSGIALTFFPALTSVAIAQFLDNSNQAETLGNYIFTLGNNGYDSAIERGRAAGAIIIAALITFAIYFTVVFLPKIVRIVHNK", "aa_hexdigest": "fa5e4ad6a602f21f6b589cb9d1636ce2", "start_type": "TTG", "rbs_motif": null, "genes": [ "potB" ], "truncated": "both", "pseudogene": { "start": 51563, "stop": 52381, "observations": { "mutation": [ 51698, 51833, 52355 ], "stop": [ 51698, 51833, 52355 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47289", "query_cov": 1.0, "subject_cov": 0.5754385964912281, "identity": 0.9940000000000001, "score": 317.0, "evalue": 9.05e-108, "gene_start": 1, "gene_end": 164, "reference_start": 114, "reference_end": 277, "reference_sequence": "MHIKKKYWLLLPFFLLMTIFFIIPMAWIIVSGLQSEDGASISQKYEPLVSGLGFFNSFWTSLWISIVTVIVALLFSFPFCYFLSQSKNKIFKAFVISIATVPIWSSFLIKLIGLKTLLDLLIGLSLNRVGDNNLTFGSGYTLLGTIYLFTPFMFLPLYNHFCVLPKNLLLASQDLGYNWIYSFVKVVIPFSKTAMLSGIALTFFPALTSVAIAQFLDNSNQAETLGNYIFTLGNNGYDSAIERGRAAGAIIIAALITFAIYFTVVFLPKIVRIVHNKWKQHEKAF" }, "paralog": false, "identity": 0.9669117647058824, "score": 508.0, "evalue": 1.39e-185, "description": "Internal stop codon at 51698, 51833, 52355. Nonsense mutation around 51698, 51833, 52355.", "psc": { "uniref90_id": "UniRef90_P47289", "gene": "potB", "product": "Spermidine/putrescine transport system permease protein PotB homolog", "uniref50_id": "UniRef50_P47289", "cog_id": "COG1176", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47289", "UniRef:UniRef50_P47289", "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47289" ], "product": "Spermidine/putrescine transport system permease protein PotB homolog" } }, "id": "KKNAAMEMLJ_138", "locus": "GCA000027325_00098" }, { "type": "cds", "sequence": "contig_1", "start": 52365, "stop": 52769, "strand": "+", "frame": 3, "gene": "potC", "product": "Spermidine/putrescine transport system permease PotC-like protein", "db_xrefs": [ "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47290", "UniRef:UniRef90_P47290" ], "nt": "ATGAAAAAGCATTTTAAGAATTTAATTAAAAACAGTTATTTCTTTCTGTTAATAACTTTAATCTATTTACCACTTTTAATAGTTGTACTTGTTAGTTTAAACGGTTCTTCTTCAAGAGGAAATATAGTGCTTGATTTTGGTAATGTTTTAAATCCTAATCCTGATTCTAAATCTGCTTATTTAAGATTAGGTGAAACTGATTTTGCAACACCACTAATAAATTCAATCATTATAGGTGTGATCACTGTTTTAGTGTCTGTTCCTATTGCTGTTATCAGTGCGTTTGCGCTTTTAAGAACAAGGAATGCTTTAAAAAAGACAATCTTTGGAATTACTAATTTTTCTTTAGCAACTCCTGATATTATTACTGCTATCTCTTTAGTGTTGTTATTTGCTAACACTTGA", "aa": "MKKHFKNLIKNSYFFLLITLIYLPLLIVVLVSLNGSSSRGNIVLDFGNVLNPNPDSKSAYLRLGETDFATPLINSIIIGVITVLVSVPIAVISAFALLRTRNALKKTIFGITNFSLATPDIITAISLVLLFANT", "aa_hexdigest": "d5eb35154948182a746979f9cd434e24", "start_type": "ATG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "3-prime", "pseudogene": { "start": 52365, "stop": 53072, "observations": { "mutation": [ 52767 ], "stop": [ 52767 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47290", "query_cov": 1.0, "subject_cov": 0.47183098591549294, "identity": 1.0, "score": 238.0, "evalue": 3.68e-77, "gene_start": 1, "gene_end": 134, "reference_start": 1, "reference_end": 134, "reference_sequence": "MKKHFKNLIKNSYFFLLITLIYLPLLIVVLVSLNGSSSRGNIVLDFGNVLNPNPDSKSAYLRLGETDFATPLINSIIIGVITVLVSVPIAVISAFALLRTRNALKKTIFGITNFSLATPDIITAISLVLLFANTWLSFNQQLGFFTIITSHISFSVPYALILIYPKIQKLNPNLILASQDLGYSPLKTFFHITLPYLMPSIFSAVLVVFATSFDDYVITSLVQGSVKTIATELYSFRKGIKAWAIAFGSILILISVLGVCLITLQKYLREKRKEIIKIRQWKNS" }, "paralog": false, "identity": 0.9957446808510638, "score": 445.0, "evalue": 3.85e-161, "description": "Internal stop codon at 52767. Nonsense mutation around 52767.", "psc": { "uniref90_id": "UniRef90_P47290", "gene": "potC", "product": "Spermidine/putrescine transport system permease protein PotC homolog", "uniref50_id": "UniRef50_P47290", "cog_id": "COG1177", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47290", "UniRef:UniRef50_P47290", "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47290" ], "product": "Spermidine/putrescine transport system permease protein PotC homolog" } }, "id": "KKNAAMEMLJ_139", "locus": "GCA000027325_00099" }, { "type": "cds", "sequence": "contig_1", "start": 52830, "stop": 53207, "strand": "+", "frame": 3, "gene": "potC", "product": "Spermidine/putrescine transport system permease PotC-like protein", "db_xrefs": [ "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47290", "UniRef:UniRef90_P47290" ], "nt": "GTGCCTTATGCATTGATTTTGATTTACCCTAAAATTCAAAAATTGAATCCTAATTTAATTCTTGCTTCTCAAGATTTAGGCTATTCGCCTTTAAAAACTTTTTTCCATATTACTCTACCTTATCTAATGCCAAGTATTTTTTCAGCAGTACTAGTAGTATTTGCAACTAGTTTTGATGATTATGTAATTACCTCTTTAGTACAAGGATCAGTAAAAACTATAGCAACTGAACTCTATTCATTTAGAAAAGGAATTAAAGCATGGGCAATCGCCTTTGGGTCTATTCTCATATTGATTAGTGTCTTAGGAGTCTGTTTAATAACCCTGCAAAAGTATTTAAGGGAAAAAAGAAAGGAAATAATCAAAATAAGACAATGA", "aa": "MPYALILIYPKIQKLNPNLILASQDLGYSPLKTFFHITLPYLMPSIFSAVLVVFATSFDDYVITSLVQGSVKTIATELYSFRKGIKAWAIAFGSILILISVLGVCLITLQKYLREKRKEIIKIRQ", "aa_hexdigest": "e75e66aadada292443606bf52a3766c4", "start_type": "GTG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "both", "pseudogene": { "start": 52530, "stop": 53219, "observations": { "mutation": [ 52767, 53205 ], "stop": [ 52767, 53205 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47290", "query_cov": 0.992, "subject_cov": 0.43661971830985913, "identity": 1.0, "score": 229.0, "evalue": 1.58e-73, "gene_start": 2, "gene_end": 125, "reference_start": 157, "reference_end": 280, "reference_sequence": "MKKHFKNLIKNSYFFLLITLIYLPLLIVVLVSLNGSSSRGNIVLDFGNVLNPNPDSKSAYLRLGETDFATPLINSIIIGVITVLVSVPIAVISAFALLRTRNALKKTIFGITNFSLATPDIITAISLVLLFANTWLSFNQQLGFFTIITSHISFSVPYALILIYPKIQKLNPNLILASQDLGYSPLKTFFHITLPYLMPSIFSAVLVVFATSFDDYVITSLVQGSVKTIATELYSFRKGIKAWAIAFGSILILISVLGVCLITLQKYLREKRKEIIKIRQWKNS" }, "paralog": false, "identity": 0.9912663755458515, "score": 427.0, "evalue": 3.86e-154, "description": "Internal stop codon at 52767, 53205. Nonsense mutation around 52767, 53205.", "psc": { "uniref90_id": "UniRef90_P47290", "gene": "potC", "product": "Spermidine/putrescine transport system permease protein PotC homolog", "uniref50_id": "UniRef50_P47290", "cog_id": "COG1177", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47290", "UniRef:UniRef50_P47290", "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47290" ], "product": "Spermidine/putrescine transport system permease protein PotC homolog" } }, "id": "KKNAAMEMLJ_140", "locus": "GCA000027325_00100" }, { "type": "cds", "sequence": "contig_1", "start": 53204, "stop": 53488, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAGTTAAAATATTGCTTTTTCTCACTTTTTGTTAGTCTCTCATCAATATTGAGTAGTTGTGGTTCAACAACATTTGTACTAGCTAACTTTGAATCTTATATTTCGCCCTTATTGCTAGAAAGAGTACAAGAAAAACATCCCTTAACTTTCTTGACTTATCCTAGTAATGAAAAACTAATTAATGGTTTTGCTAACAACACTTATTCAGTAGCAGTAGCATCTACTTATGCAGTTAGTGAATTGATAGAAAGGGATCTATTATCACCAATAGATTGA", "aa": "MKKQLKYCFFSLFVSLSSILSSCGSTTFVLANFESYISPLLLERVQEKHPLTFLTYPSNEKLINGFANNTYSVAVASTYAVSELIERDLLSPID", "aa_hexdigest": "eac83ea179219eaf3c77ac5f95e39326", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10552.020999999988, "isoelectric_point": 5.723197746276856 }, "id": "KKNAAMEMLJ_141", "locus": "GCA000027325_00101" }, { "type": "cds", "sequence": "contig_1", "start": 53735, "stop": 54568, "strand": "+", "frame": 2, "gene": "potD", "product": "putative lipoprotein MG045", "db_xrefs": [ "COG:COG0687", "COG:E", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75056", "UniRef:UniRef90_P47291" ], "nt": "GTGAAACACAAAGATCGCTTTAATGACAATAGGTTAGTTTTCATTGATGATGCTAGAACGATCTTTTCACTTGCTAACATCGTTAATACTAACAACAATTCAGCTGATGTTAATCCAAAGGAAGATGGAATTGGTTATTTCACTAATGTCTATGAAAGCTTTCAAAGACTTGGATTAACAAAATCTAATTTAGATAGTATCTTTGTTAATTCTGATTCCAATATTGTGATCAATGAATTGGCAAGTGGTAGAAGACAAGGAGGAATTGTTTACAATGGTGATGCAGTGTATGCTGCATTGGGCGGTGATTTACGTGATGAATTGAGTGAAGAACAGATTCCTGATGGGAACAACTTTCACATTGTGCAACCCAAAATTTCCCCAGTTGCTTTAGATCTTTTGGTTATCAATAAACAACAATCTAATTTTCAAAAAGAAGCACATGAGATCATTTTTGATCTTGCTTTGGATGGTGCTGATCAAACTAAAGAACAGTTAATTAAAACTGATGAAGAATTGGGTACTGATGATGAAGACTTTTACTTAAAAGGAGCGATGCAAAACTTTAGTTATGTGAACTATGTTTCACCATTAAAAGTAATATCTGATCCAAGTACTGGAATAGTCAGTTCCAAAAAGAATAATGCTGAAATGAAAAGTAAACAAATGTCAACTGATCAAATGACTAGTGAAAAAGAATTTGATTATTACACTGAAACACTTAAAGCATTATTAGAGAAAGAAGATAGTGCAGAATTAAATGAAAATGAAAAAAAACTAGTTGAAACCATTAAGAAAGCTTACACTATTGAAAAAGATAGTTCAATTCGGTGA", "aa": "MKHKDRFNDNRLVFIDDARTIFSLANIVNTNNNSADVNPKEDGIGYFTNVYESFQRLGLTKSNLDSIFVNSDSNIVINELASGRRQGGIVYNGDAVYAALGGDLRDELSEEQIPDGNNFHIVQPKISPVALDLLVINKQQSNFQKEAHEIIFDLALDGADQTKEQLIKTDEELGTDDEDFYLKGAMQNFSYVNYVSPLKVISDPSTGIVSSKKNNAEMKSKQMSTDQMTSEKEFDYYTETLKALLEKEDSAELNENEKKLVETIKKAYTIEKDSSIR", "aa_hexdigest": "a31cc115ef9295240317c8e38aef5917", "start_type": "GTG", "rbs_motif": null, "genes": [ "potD" ], "truncated": "both", "pseudogene": { "start": 53435, "stop": 54652, "observations": { "mutation": [ 53486, 53627, 53711, 54566, 54626 ], "stop": [ 53486, 53627, 53711, 54566, 54626 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47291", "query_cov": 1.0, "subject_cov": 0.5734989648033126, "identity": 0.996, "score": 521.0, "evalue": 2.84e-183, "gene_start": 1, "gene_end": 277, "reference_start": 178, "reference_end": 454, "reference_sequence": "MKKQLKYCFFSLFVSLSSILSSCGSTTFVLANFESYISPLLLERVQEKHPLTFLTYPSNEKLINGFANNTYSVAVASTYAVSELIERDLLSPIDWSQFNLKKSSSSSDKVNNASDAKDLFIDSIKEISQQTKDSKNNELLHWAVPYFLQNLVFVYRGEKISELEQENVSWTDVIKAIVKHKDRFNDNRLVFIDDARTIFSLANIVNTNNNSADVNPKEDGIGYFTNVYESFQRLGLTKSNLDSIFVNSDSNIVINELASGRRQGGIVYNGDAVYAALGGDLRDELSEEQIPDGNNFHIVQPKISPVALDLLVINKQQSNFQKEAHEIIFDLALDGADQTKEQLIKTDEELGTDDEDFYLKGAMQNFSYVNYVSPLKVISDPSTGIVSSKKNNAEMKSKQMSTDQMTSEKEFDYYTETLKALLEKEDSAELNENEKKLVETIKKAYTIEKDSSIRWNQLVEKPISPLQRSNLSLSWLDFKLHWW" }, "paralog": false, "identity": 0.9876543209876543, "score": 771.0, "evalue": 2.56e-284, "description": "Internal stop codon at 53486, 53627, 53711, 54566, 54626. Nonsense mutation around 53486, 53627, 53711, 54566, 54626.", "psc": { "uniref90_id": "UniRef90_P47291", "gene": "PotD", "product": "Uncharacterized lipoprotein MG045", "uniref50_id": "UniRef50_P75056", "cog_id": "COG0687", "cog_category": "E", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47291", "UniRef:UniRef50_P75056", "COG:COG0687", "COG:E", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75056" ], "product": "Uncharacterized lipoprotein MG045 homolog" } }, "id": "KKNAAMEMLJ_142", "locus": "GCA000027325_00102" }, { "type": "cds", "sequence": "contig_1", "start": 54657, "stop": 55271, "strand": "+", "frame": 3, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711", "SO:0001217", "UniRef:UniRef50_P75055", "UniRef:UniRef90_P47292" ], "nt": "ATGGAACAACCGTTGTGTGTTTTAGGGATTGAAACAACCTGTGATGATACAGGTCTTAGTATTGTCATTGATCAAAAAATCAAGAGTAACATTGTTATCTCTTCTGCTAACTTACATGTAAAAACAGGAGGAGTTGTACCTGAAATTGCAGCACGATGCCACGAACAAAATCTCTTTAAAGCAATAAGAGATTTAAATTTTGAGATAAGAGATTTATCTCACATTGCTTATGCATGTAATCCTGGGTTAGCAGGATGTTTACATGTGGGAGCCACTTTTGCTAGAAGCTTAAGTTTCTTATTAGACAAACCATTGTTACCCATCAACCATCTTTATGCGCATATCTTTTCTTGTTTAATTGATCAAGATTTAAATAAGCTGCAATTACCAGCATTAGGCCTTGTAATTTCAGGTGGACATACTGCCATTTATCTAGTTAAATCATTTTATGAACTTGAACTAATTGCTGAAACTAGTGATGATGCAATTGGTGAAGTTTATGACAAGATAGGCAGAGCAATGGGCTTTGATTATCCTGCTGGTAGTAAAATTGATAGTCTTTTTAATAAAGAATTAGTTAAACCTCACTATTTCTTTAAACCTTCTACTAAGTGA", "aa": "MEQPLCVLGIETTCDDTGLSIVIDQKIKSNIVISSANLHVKTGGVVPEIAARCHEQNLFKAIRDLNFEIRDLSHIAYACNPGLAGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSCLIDQDLNKLQLPALGLVISGGHTAIYLVKSFYELELIAETSDDAIGEVYDKIGRAMGFDYPAGSKIDSLFNKELVKPHYFFKPSTK", "aa_hexdigest": "276fba995d1708c809dbb1bf246e6bd0", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 54657, "stop": 55574, "observations": { "mutation": [ 55269 ], "stop": [ 55269 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47292", "query_cov": 1.0, "subject_cov": 0.6476190476190476, "identity": 1.0, "score": 416.0, "evalue": 1.41e-145, "gene_start": 1, "gene_end": 204, "reference_start": 1, "reference_end": 204, "reference_sequence": "MEQPLCVLGIETTCDDTGLSIVIDQKIKSNIVISSANLHVKTGGVVPEIAARCHEQNLFKAIRDLNFEIRDLSHIAYACNPGLAGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSCLIDQDLNKLQLPALGLVISGGHTAIYLVKSFYELELIAETSDDAIGEVYDKIGRAMGFDYPAGSKIDSLFNKELVKPHYFFKPSTKWTKFSYSGLKSQCLNKIKQISANKTRIDWSELASNFQATIIDHYIDHVKNAIKKFAPKMLLVGGGVSANSYLSNRISTLNLPFLIADSKYTSDNGAMIGFYASLLINGDKN" }, "paralog": false, "identity": 0.9967213114754099, "score": 608.0, "evalue": 1.02e-223, "description": "Internal stop codon at 55269. Nonsense mutation around 55269.", "psc": { "uniref90_id": "UniRef90_P47292", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P75055", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47292", "EC:2.3.1.234", "UniRef:UniRef50_P75055", "COG:COG0533", "COG:J", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ] }, "pscc": { "uniref50_id": "UniRef50_P75055", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75055" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "KKNAAMEMLJ_143", "locus": "GCA000027325_00103" }, { "type": "cds", "sequence": "contig_1", "start": 55443, "stop": 55604, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTAGTAGGAGGTGGAGTTAGTGCCAATTCTTATCTATCTAACAGAATTAGTACATTAAATTTACCCTTTTTAATTGCTGATAGCAAATACACCAGTGATAATGGAGCAATGATTGGTTTTTATGCATCACTTTTAATTAATGGCGATAAGAATTAA", "aa": "MLLVGGGVSANSYLSNRISTLNLPFLIADSKYTSDNGAMIGFYASLLINGDKN", "aa_hexdigest": "6946ae4a63279b6a7e7fea716897fbf0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5598.319300000001, "isoelectric_point": 5.793621253967285 }, "id": "KKNAAMEMLJ_144", "locus": "GCA000027325_00104" }, { "type": "cds", "sequence": "contig_1", "start": 55588, "stop": 55818, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00438.25" ], "nt": "ATGGCGATAAGAATTAAAAGTACAAGAGTTGGTAGATTTGTTTCTGAATCAGTGGGATTAGGTCATCCTGATAAAATTTGTGATCAGATTGCAGATAGTATCTTAGACCAATGTTTACTACAGAGTAAAACTAGTCATGTAGCATGTGAAGTCTTTGCTTCTAAAAACCTTATTTTAATAGGTGGTGAGATTTCAACAAGTGGCTATGTTGATGTTGTTCAAACTGCTTGA", "aa": "MAIRIKSTRVGRFVSESVGLGHPDKICDQIADSILDQCLLQSKTSHVACEVFASKNLILIGGEISTSGYVDVVQTA", "aa_hexdigest": "be4374b706773c2248f95f85620d3c9e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00438.25", "name": "S-AdoMet_synt_N", "length": 63, "aa_cov": 0.8289473684210527, "hmm_cov": 1.0, "evalue": 5.1623115595508315e-26, "score": 86.24707794189453, "start": 13, "stop": 75 } ], "seq_stats": { "molecular_weight": 8121.262299999996, "isoelectric_point": 6.01432704925537 }, "id": "KKNAAMEMLJ_145", "locus": "GCA000027325_00105" }, { "type": "cds", "sequence": "contig_1", "start": 55963, "stop": 56478, "strand": "+", "frame": 1, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "COG:COG0192", "COG:H", "EC:2.5.1.6", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005829", "GO:0006556", "GO:0006730", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_P47293", "UniRef:UniRef90_P47293" ], "nt": "GTGAATGAAACAAAGCAACTAATGCCTTTAGGAGTTTTACTAGCACACTCGTTTTTAAAACAAGCAGAAAAACTAACAAAACAATTTGATTTTTTAAAAAATGATATGAAAAGTCAAGTGGTTTTAAACTACAGTTTAAACCAAGTTGAATGTGAAGAAGTTTTACTATCAATTCAACACACTAATGCTATTAGTTTAACAGAATTGAGAAAAGTGATTGAAAATAATGTAATTCTACCTGTTTTAAACCAATATGGTTTTCAAGATAAAAAGCCAACTTGTTTAGTGAATCCTGGTGGTTCTTTTGTTTTAGGTGGACCTATGGCAGATACTGGACTAACTGGTAGAAAAATCATTGTTGACACCTATGGTCCATATGCTCACCATGGTGGTGGTAGCTTTAGTGGCAAAGATCCTAGTAAGGTGGATAGAACAGGTGCTTATTTTGCACGTTTTATCGCAAAACATATTGTAAGTTTAGGCTGGGCCAGTGAGTGTGAAGTCAGTATTAGCTGA", "aa": "MNETKQLMPLGVLLAHSFLKQAEKLTKQFDFLKNDMKSQVVLNYSLNQVECEEVLLSIQHTNAISLTELRKVIENNVILPVLNQYGFQDKKPTCLVNPGGSFVLGGPMADTGLTGRKIIVDTYGPYAHHGGGSFSGKDPSKVDRTGAYFARFIAKHIVSLGWASECEVSIS", "aa_hexdigest": "7cbf5654cc2b87a4fbb0719aa3d9d84b", "start_type": "GTG", "rbs_motif": null, "genes": [ "metK" ], "truncated": "both", "pseudogene": { "start": 55663, "stop": 56739, "observations": { "mutation": [ 55816, 56476, 56584, 56683 ], "stop": [ 55816, 56476, 56584, 56683 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47293", "query_cov": 0.9941520467836257, "subject_cov": 0.44386422976501305, "identity": 1.0, "score": 343.0, "evalue": 2.92e-116, "gene_start": 2, "gene_end": 171, "reference_start": 127, "reference_end": 296, "reference_sequence": "MAIRIKSTRVGRFVSESVGLGHPDKICDQIADSILDQCLLQSKTSHVACEVFASKNLILIGGEISTSGYVDVVQTAWRILRNLGYNETDFSFLSCINNQSLEINQAVLKNNEINAGDQGITVGYAVNETKQLMPLGVLLAHSFLKQAEKLTKQFDFLKNDMKSQVVLNYSLNQVECEEVLLSIQHTNAISLTELRKVIENNVILPVLNQYGFQDKKPTCLVNPGGSFVLGGPMADTGLTGRKIIVDTYGPYAHHGGGSFSGKDPSKVDRTGAYFARFIAKHIVSLGWASECEVSISWVFSKPNPQSITVKCFNTNIQYDEVLINRVVNNYFNWSITKIIDKLKLLDFVKYSDYAVYGHFGNDLSPWEQPTELDKLECLIKNFH" }, "paralog": false, "identity": 0.9888268156424581, "score": 704.0, "evalue": 2.01e-261, "description": "Internal stop codon at 55816, 56476, 56584, 56683. Nonsense mutation around 55816, 56476, 56584, 56683.", "psc": { "uniref90_id": "UniRef90_P47293", "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_P47293", "cog_id": "COG0192", "cog_category": "H", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005829", "GO:0006556", "GO:0006730" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47293", "EC:2.5.1.6", "UniRef:UniRef50_P47293", "COG:COG0192", "COG:H", "KEGG:K00789", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005829", "GO:0006556", "GO:0006730" ] }, "pscc": { "uniref50_id": "UniRef50_P47293", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47293" ], "product": "S-adenosylmethionine synthase" } }, "id": "KKNAAMEMLJ_146", "locus": "GCA000027325_00106" }, { "type": "cds", "sequence": "contig_1", "start": 56715, "stop": 56972, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_009885710.1", "SO:0001217", "UniParc:UPI0001B41721", "UniRef:UniRef100_UPI0001B41721", "UniRef:UniRef50_UPI0001B41721", "UniRef:UniRef90_UPI0001B41721" ], "nt": "ATGCTTAATCAAAAATTTCCATTAGATCCTAATAAAAAAAAGGAACAACAAGGCGTAGTAAAACCAAATCTACCGGTTGTTAAAGAAAAGAAAAAACAGCCATTTAAAAAACCAAATTGGAGTGAATTTAAGCTGTTCAACTTTTTTAAAAAGCACACTTATTTTTGCTTGGTTGTATTAGCTTTTCTTATCTTGATTATTTTACTTAGCAGCTTATTTGCTATTCCACTTAGCCAAATACCTTCAAGTACTGTTTAA", "aa": "MLNQKFPLDPNKKKEQQGVVKPNLPVVKEKKKQPFKKPNWSEFKLFNFFKKHTYFCLVVLAFLILIILLSSLFAIPLSQIPSSTV", "aa_hexdigest": "b41c1537ab0f3685e9bf9b4b5b478b4b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001B41721", "ncbi_nrp_id": "WP_009885710.1", "uniref100_id": "UniRef100_UPI0001B41721", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001B41721", "RefSeq:WP_009885710.1", "UniRef:UniRef100_UPI0001B41721" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001B41721", "uniref90_id": "UniRef90_UPI0001B41721" }, "psc": { "uniref90_id": "UniRef90_UPI0001B41721", "uniref50_id": "UniRef50_UPI0001B41721" }, "pscc": { "uniref50_id": "UniRef50_UPI0001B41721", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B41721" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9889.837899999991, "isoelectric_point": 10.067270088195798 }, "id": "KKNAAMEMLJ_147", "locus": "GCA000027325_00107" }, { "type": "cds", "sequence": "contig_1", "start": 57056, "stop": 57175, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGACTAGAGAAGAAAGGAGACATCCCAAATTAATTAATCGTGATCCTAATAGAAAACAGCGCATCATAAAAGGATCAGGGAGAAAAATGGATGAGTTAAACAAACTGATGAAGGAATGA", "aa": "MTREERRHPKLINRDPNRKQRIIKGSGRKMDELNKLMKE", "aa_hexdigest": "ad263c070bd32515b7247a288903f725", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 39, "aa_cov": 1.0, "hmm_cov": 0.9487179487179487, "evalue": 7.877776000644393e-12, "score": 40.56576919555664, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 4803.603900000001, "isoelectric_point": 10.963251686096193 }, "id": "KKNAAMEMLJ_148", "locus": "GCA000027325_00108" }, { "type": "cds", "sequence": "contig_1", "start": 57194, "stop": 57916, "strand": "-", "frame": 2, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501", "KEGG:K03106", "SO:0001217", "UniRef:UniRef50_P75054", "UniRef:UniRef90_P47294" ], "nt": "ATGTTAGTAGGCTTGGACATCTACAGACCCGCTGCCATTGAACAACTTGAAACGCTTTCACAACAAACTAACAGCGTATTTTTTGCACAAGGCACTCAACCAGTTGCTAAAACAACAAAAGCAGCACTCAGTGCTTTTAAAACTGCAAAATGTCAAACAATCATTTGTGATACCGCTGGTAGATTACAAACAAATGAAACATTAATGGATGAATTGGTAAGTGTTAAAAATGAATTAAATCCTGATGAAATTATCATGGTAGTAGATGGATTAAGCGGTCAGGAAATTATCAATGTTGCTCAAACGTTCCACAAACGTTTAAAACTAACTGGATTTATTATCAGTAAATTAGACAGTGATGCTAGAGCAGGAGCTGCACTTTCATTAGCTTCACTTTTACAAGTACCCATTAAATTAATTGGTGTTTCTGAAAAATTAGATGGATTGGAACAATTTCATCCTGAAAGGATAGCCAATCGGATCTTAGGTTTGGGTGATGTAATGAGTTTAGTTGAAAAAGCTGAACAAGTTTTTGATAAAAAAGATTTAACTAAAACCATCAGCAAGATGTTTTTGGGAAAAATGGATTTAGAAGATCTTTTGATCTACATGCAACAAATGCACAAAATGGGAAGTGTCAGTTCACTGATAAAAATGTTGCCTGCTAACTTTTCTGTATCAGAAGAAAATGCTGAATTAATTGAAAACAAAATTGAACTATGA", "aa": "MLVGLDIYRPAAIEQLETLSQQTNSVFFAQGTQPVAKTTKAALSAFKTAKCQTIICDTAGRLQTNETLMDELVSVKNELNPDEIIMVVDGLSGQEIINVAQTFHKRLKLTGFIISKLDSDARAGAALSLASLLQVPIKLIGVSEKLDGLEQFHPERIANRILGLGDVMSLVEKAEQVFDKKDLTKTISKMFLGKMDLEDLLIYMQQMHKMGSVSSLIKMLPANFSVSEENAELIENKIEL", "aa_hexdigest": "f5f8e6ab771b0e461e8d6a3234e079db", "start_type": "ATG", "rbs_motif": null, "genes": [ "ffh" ], "truncated": "both", "pseudogene": { "start": 56969, "stop": 58216, "observations": { "mutation": [ 57058, 57196, 57946 ], "stop": [ 57058, 57196, 57946 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47294", "query_cov": 1.0, "subject_cov": 0.5381165919282511, "identity": 1.0, "score": 416.0, "evalue": 7.72e-143, "gene_start": 1, "gene_end": 240, "reference_start": 132, "reference_end": 371, "reference_sequence": "MFKAMLSSIVMRTMQKKINAQTITEKDVELVLKEIRIALLDADVNLLVVKNFIKAIRDKTVGQTIEPGQDLQKSLLKTIKTELINILSQPNQELNEKRPLKIMMVGLQGSGKTTTCGKLAYWLEKKYKQKTMLVGLDIYRPAAIEQLETLSQQTNSVFFAQGTQPVAKTTKAALSAFKTAKCQTIICDTAGRLQTNETLMDELVSVKNELNPDEIIMVVDGLSGQEIINVAQTFHKRLKLTGFIISKLDSDARAGAALSLASLLQVPIKLIGVSEKLDGLEQFHPERIANRILGLGDVMSLVEKAEQVFDKKDLTKTISKMFLGKMDLEDLLIYMQQMHKMGSVSSLIKMLPANFSVSEENAELIENKIELWKVLINSMTREERRHPKLINRDPNRKQRIIKGSGRKMDELNKLMKEWNKMQLKATEMGKLLKTGSNPFGGFGQFF" }, "paralog": false, "identity": 0.9927710843373494, "score": 752.0, "evalue": 4.31e-278, "description": "Internal stop codon at 57058, 57196, 57946. Nonsense mutation around 57058, 57196, 57946.", "psc": { "uniref90_id": "UniRef90_P47294", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P75054", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47294", "EC:3.6.5.4", "UniRef:UniRef50_P75054", "COG:COG0541", "COG:U", "KEGG:K03106", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_P75054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75054" ], "product": "Signal recognition particle protein" } }, "id": "KKNAAMEMLJ_149", "locus": "GCA000027325_00109" }, { "type": "cds", "sequence": "contig_1", "start": 57944, "stop": 58270, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28", "PFAM:PF02881.24" ], "nt": "ATGCAAAAAAAAATTAACGCTCAAACGATCACTGAGAAAGATGTAGAGTTAGTTCTAAAAGAGATTAGAATTGCATTGCTTGATGCTGATGTTAACCTGCTTGTTGTTAAAAATTTCATCAAAGCAATTAGAGATAAAACAGTAGGACAAACCATTGAACCTGGTCAAGATTTGCAAAAGTCTCTATTAAAAACAATCAAAACAGAACTAATTAATATCTTAAGCCAACCCAACCAAGAACTAAATGAAAAAAGACCTTTAAAAATAATGATGGTTGGTTTACAAGGATCAGGTAAAACAACAACTTGTGGCAAACTAGCTTATTGA", "aa": "MQKKINAQTITEKDVELVLKEIRIALLDADVNLLVVKNFIKAIRDKTVGQTIEPGQDLQKSLLKTIKTELINILSQPNQELNEKRPLKIMMVGLQGSGKTTTCGKLAY", "aa_hexdigest": "57f2a1083aea8c7b12f9058303ff6528", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 20, "aa_cov": 0.18518518518518517, "hmm_cov": 1.0, "evalue": 1.4649876883851692e-08, "score": 29.62334632873535, "start": 88, "stop": 107 }, { "id": "PF02881.24", "name": "SRP54_N", "length": 57, "aa_cov": 0.5185185185185185, "hmm_cov": 1.0, "evalue": 1.7043736042371655e-15, "score": 52.13430404663086, "start": 7, "stop": 62 } ], "seq_stats": { "molecular_weight": 12073.171199999988, "isoelectric_point": 9.443602943420409 }, "id": "KKNAAMEMLJ_150", "locus": "GCA000027325_00110" }, { "type": "cds", "sequence": "contig_1", "start": 58284, "stop": 58691, "strand": "+", "frame": 3, "gene": null, "product": "Uridine phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9D1M2H1" ], "nt": "ATGCTTGATAACATTGCTTTGAACATGATTTTTAATTATTTATTATTAAATAATGTTTTAATAAAACAATATTGCAATATGACCCCACATATAAGTGCTAAGAAAGATGACATTAGCAAAGTTGTTTTAATGCCAGGTGATCCATTGAGAGCTAAATGGATAGCTGAGCAATTCTTAGATCAAGCTAAATTAGTCAATGAAGTGAGGGGAATGTTTGCTTATACTGGGCAGTATAAATCTAAAACAGTTACAGTAATGGGCCATGGAATGGGGATCCCTTCTATTGGAATTTATTCATATGAGTTGATGAATTTTTATGAGGTTGAAACTATCATTAGAATCGGAAGTTGTGGTGCTTTAGCACCGCAATTAAAATTAAAAGATCTTGTTATTGCTTCAAAAGCATGA", "aa": "MLDNIALNMIFNYLLLNNVLIKQYCNMTPHISAKKDDISKVVLMPGDPLRAKWIAEQFLDQAKLVNEVRGMFAYTGQYKSKTVTVMGHGMGIPSIGIYSYELMNFYEVETIIRIGSCGALAPQLKLKDLVIASKA", "aa_hexdigest": "5e6b0e9cbc242a2599f07e5f94feb7ac", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FEB9C95", "query_cov": 0.8074074074074075, "subject_cov": 0.8449612403100775, "identity": 0.624, "score": 143.0, "evalue": 1.4e-41, "valid": false, "uniref50_id": "UniRef50_A0A9D1M2H1" }, "pscc": { "uniref50_id": "UniRef50_A0A9D1M2H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9D1M2H1" ], "product": "Uridine phosphorylase" }, "genes": [], "id": "KKNAAMEMLJ_151", "locus": "GCA000027325_00111" }, { "type": "cds", "sequence": "contig_1", "start": 58716, "stop": 59078, "strand": "+", "frame": 3, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152", "KEGG:K03784", "SO:0001217", "UniRef:UniRef50_P47295", "UniRef:UniRef90_P47295" ], "nt": "ATGGGTGTTGAAATTCCAGAAGATAAGATCTTATTTGCAACAAGTTCTTTAGTGGAATTAGCAAAAGAAACTGCGATTAAGAACAAGCTTGATTTTCATGAAGGATTAGTATTTTGTGAGGATGCTTTTTATCAAACTAGAAAAGATGTAATTAGTCTTGCTAAAGAAAAAAATAGTTTAGCAGTTGAAATGGAAGCACATGCACTTTATGCTAATGCAATCCTGTTGAAGAAAAAAGCACTTACACTCTTAACAGTATCTGATTCTCTAGTAACTCATGAAGCACTTAGTTCTGAATTAAGACAAAAGTCATTTAAGCAAATGGCTTTATTAGCACTTGAAATGACTCAAAAACTAATCTAA", "aa": "MGVEIPEDKILFATSSLVELAKETAIKNKLDFHEGLVFCEDAFYQTRKDVISLAKEKNSLAVEMEAHALYANAILLKKKALTLLTVSDSLVTHEALSSELRQKSFKQMALLALEMTQKLI", "aa_hexdigest": "b5531ca3f8d05936b458016ffb9425be", "start_type": "ATG", "rbs_motif": null, "genes": [ "deoD" ], "truncated": "5-prime", "pseudogene": { "start": 58416, "stop": 59078, "observations": { "mutation": [ 58689 ], "stop": [ 58689 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47295", "query_cov": 1.0, "subject_cov": 0.5042016806722689, "identity": 1.0, "score": 221.0, "evalue": 2.33e-71, "gene_start": 1, "gene_end": 120, "reference_start": 119, "reference_end": 238, "reference_sequence": "MTPHISAKKDDISKVVLMPGDPLRAKWIAEQFLDQAKLVNEVRGMFAYTGQYKSKTVTVMGHGMGIPSIGIYSYELMNFYEVETIIRIGSCGALAPQLKLKDLVIASKAWSESIYAKDMGVEIPEDKILFATSSLVELAKETAIKNKLDFHEGLVFCEDAFYQTRKDVISLAKEKNSLAVEMEAHALYANAILLKKKALTLLTVSDSLVTHEALSSELRQKSFKQMALLALEMTQKLI" }, "paralog": false, "identity": 0.9954545454545455, "score": 421.0, "evalue": 2.37e-152, "description": "Internal stop codon at 58689. Nonsense mutation around 58689.", "psc": { "uniref90_id": "UniRef90_P47295", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_P47295", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47295", "EC:2.4.2.1", "UniRef:UniRef50_P47295", "COG:COG0813", "COG:F", "KEGG:K03784", "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152" ] }, "pscc": { "uniref50_id": "UniRef50_P47295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47295" ], "product": "Purine nucleoside phosphorylase DeoD-type" } }, "id": "KKNAAMEMLJ_152", "locus": "GCA000027325_00112" }, { "type": "cds", "sequence": "contig_1", "start": 59082, "stop": 59753, "strand": "+", "frame": 3, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_010869307.1", "SO:0001217", "UniParc:UPI00001291A6", "UniRef:UniRef100_P47296", "UniRef:UniRef50_P09924", "UniRef:UniRef90_P47296" ], "nt": "ATGAAATTAGAATACAACCGGATTATTGATAGCACCTTAGTCAAAGCTGATACGCTTCCCCATGAAATAGATACTTTATGTGCTGATGCTCATAAATACCAGTTTTTTGCAGTGTGTGTTAATCCTAGTTATGTTAGTTATGCTAAAAACATCTTGAAAAATACTGCAGTTCAACTCTGTTGTGTTGTTGGTTTCCCCTTAGGACAAACAACCCAAAAACAGAAGGTATATGAAGCTAAGATTGCTATTAAAGAGGGAGCGGATGAAATTGATATGGTAATGAATATTGCTGAGTTTAAAAAACGTTGTGCTTGTGTTATTACTGAAATTAGAGCTGTTAAAAAAGTGTGTGGCAAGCGTAAATTAAAAGTAATTATTGAAACTGCACTTTTAACAAATGATGAAATCAAAGATGCAGTTAATGTTTGCATTGATGGCAATGCAGATTATGTTAAAACTTCCACTGGTTTTTCTTTCCGTGGTGCATCTTTAGAAGATGTTCAGATTATGAATAATGCTGCAGCAAATTTAATTAAAATCAAAGCTTCAGGTGGGATTAAAACAGCAAAGCAATTTATAGATTTATTTCAAGCTGGAGCTAGTAGAATTGGAACTTCAAATGCGGTCCAAATAATGCAAGAATTAAAAAAAATGAACCATGAATATCATTAA", "aa": "MKLEYNRIIDSTLVKADTLPHEIDTLCADAHKYQFFAVCVNPSYVSYAKNILKNTAVQLCCVVGFPLGQTTQKQKVYEAKIAIKEGADEIDMVMNIAEFKKRCACVITEIRAVKKVCGKRKLKVIIETALLTNDEIKDAVNVCIDGNADYVKTSTGFSFRGASLEDVQIMNNAAANLIKIKASGGIKTAKQFIDLFQAGASRIGTSNAVQIMQELKKMNHEYH", "aa_hexdigest": "0788dbd1f6f5fd971cf468eb96eba587", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001291A6", "ncbi_nrp_id": "WP_010869307.1", "uniref100_id": "UniRef100_P47296", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001291A6", "RefSeq:WP_010869307.1", "UniRef:UniRef100_P47296" ] }, "ips": { "uniref100_id": "UniRef100_P47296", "uniref90_id": "UniRef90_P47296" }, "psc": { "uniref90_id": "UniRef90_P47296", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_P09924", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_P09924", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P09924" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "KKNAAMEMLJ_153", "locus": "GCA000027325_00113" }, { "type": "cds", "sequence": "contig_1", "start": 59740, "stop": 60663, "strand": "+", "frame": 1, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "nt": "ATGAATATCATTAATTTAATTAATAAAAAACAACGCGGAAAAGCTTTAAATTTAGCTGAAATCAATTGGTTTGTTAATGCTGTTTTAAACAAAACCATTGCTGATTATCAAATTACTGCATTTTTGATGGCTATTTGGTTTAAAGGGATGAACCCAAATGAACTTTTTTTATTAACAAAAGCAATGGTGGATACTGGTGAAATTATTAAGTTTAATCACCATGGCAAGATTAGTGTTGATAAACATTCAACTGGTGGTATTGGTGATAAGGTTTCTTTAGCATTGGTTCCTATCTTAACTAGTTTAGGATTTAGTGTTGCTAAATTATCAGGAAGAGGCCTTGGTTATACTGGTGGAACAATTGATAAATTAGAAGCAGTTGGAGTTAAAACAGAATTAACTGACCAACAAGCACAAGCATGTTTAGATAAAAATGATTGTTTTATCATCGGACAAAGTAAGGACATCGCACCAGTTGATAAAGTACTTTATGGTTTAAGAGATATTACTGGAACAGTTGATAGTTTGCCTTTAATTGCATCTAGTATTATGTCTAAAAAGCTAGCAGTTATGAACGAGTATATTTTCATTGATCTTAAATATGGAAAAGGTGCCTTTTGTAAAACTAAGAAAATTGCTAACGAACTTGCAAAACTGATGCAAAGTATTGCTAAAAGTTTTAAAAGAAAGCTGTCTGTTAAATTAAGTGATATGAATCAAGTACTTGGTAAAGCTGTTGGCAATGTAATTGAAGTTAATGAAGCTGTTAACTTTCTAAAACAAGATTTAGATCAAGTAGGACAAGATTTTATTGATTTAATGCAAACAATTGTTATTAACATTCTACTTGAAACAAAACAAGCAAAAACCAAACAAAAGGCTATTGAACTTTATCAGGATGTTTTAACTAGTAAAAAAGCATGA", "aa": "MNIINLINKKQRGKALNLAEINWFVNAVLNKTIADYQITAFLMAIWFKGMNPNELFLLTKAMVDTGEIIKFNHHGKISVDKHSTGGIGDKVSLALVPILTSLGFSVAKLSGRGLGYTGGTIDKLEAVGVKTELTDQQAQACLDKNDCFIIGQSKDIAPVDKVLYGLRDITGTVDSLPLIASSIMSKKLAVMNEYIFIDLKYGKGAFCKTKKIANELAKLMQSIAKSFKRKLSVKLSDMNQVLGKAVGNVIEVNEAVNFLKQDLDQVGQDFIDLMQTIVINILLETKQAKTKQKAIELYQDVLTSKKA", "aa_hexdigest": "dbd041b3cc27638aecf487ace3562e61", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A66C754", "query_cov": 0.8045602605863192, "subject_cov": 1.0, "identity": 0.514, "score": 236.0, "evalue": 5.88e-74, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A354QR12", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A354QR12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "KKNAAMEMLJ_154", "locus": "GCA000027325_00114" }, { "type": "cds", "sequence": "contig_1", "start": 60841, "stop": 61005, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "ATGAAGAAAACAGATCAAATTGATCCAATGGCAGGGTTATTTTTAATGAAAAAAGATAATGAGTCTGTGGCAGTTGGAGACACTGTATTAAACCTGTATAGTTCTAGTCCTATTAGCAATGAATATATCTCTGCTGCTCAAAAAACAATAATTATTAATAAATAA", "aa": "MKKTDQIDPMAGLFLMKKDNESVAVGDTVLNLYSSSPISNEYISAAQKTIIINK", "aa_hexdigest": "ac1b7ed80d423bfb0947ccfdb73aaaab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 53, "aa_cov": 0.9629629629629629, "hmm_cov": 1.0, "evalue": 2.581539328328613e-13, "score": 44.597408294677734, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5932.792799999999, "isoelectric_point": 6.026149559020996 }, "id": "KKNAAMEMLJ_155", "locus": "GCA000027325_00115" }, { "type": "cds", "sequence": "contig_1", "start": 61014, "stop": 61406, "strand": "+", "frame": 3, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802", "KEGG:K01489", "RefSeq:WP_010869308.1", "SO:0001217", "UniParc:UPI0000127407", "UniRef:UniRef100_P47298", "UniRef:UniRef50_P75051", "UniRef:UniRef90_P47298" ], "nt": "ATGAAGGTGAATTTAGAGTGGATAATTAAACAGTTACAAATGATAGTTAAAAGAGCATATACTCCCTTTTCTAACTTTAAAGTTGCATGTATGATTATTGCTAACAACCAAACTTTTTTTGGAGTTAACATTGAAAATTCTTCCTTTCCAGTAACTTTGTGTGCTGAAAGAAGCGCCATTGCTAGCATGGTTACAAGTGGTCATAGGAAAATTGATTATGTTTTTGTTTACTTCAATACTAAAAATAAGAGTAACTCACCCTGTGGAATGTGCAGACAAAACTTACTGGAATTTTCCCATCAAAAAACAAAGCTTTTTTGTATTGATAATGATAGTAGTTATAAACAATTTTCCATTGATGAATTATTAATGAATGGTTTTAAAAAGAGCTAA", "aa": "MKVNLEWIIKQLQMIVKRAYTPFSNFKVACMIIANNQTFFGVNIENSSFPVTLCAERSAIASMVTSGHRKIDYVFVYFNTKNKSNSPCGMCRQNLLEFSHQKTKLFCIDNDSSYKQFSIDELLMNGFKKS", "aa_hexdigest": "f7eb58a0e726f681ef33ce0e9201bbeb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000127407", "ncbi_nrp_id": "WP_010869308.1", "uniref100_id": "UniRef100_P47298", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000127407", "RefSeq:WP_010869308.1", "UniRef:UniRef100_P47298" ] }, "ips": { "uniref100_id": "UniRef100_P47298", "uniref90_id": "UniRef90_P47298" }, "psc": { "uniref90_id": "UniRef90_P47298", "gene": "cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_P75051", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802" ] }, "pscc": { "uniref50_id": "UniRef50_P75051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75051" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "KKNAAMEMLJ_156", "locus": "GCA000027325_00116" }, { "type": "cds", "sequence": "contig_1", "start": 61406, "stop": 62353, "strand": "+", "frame": 2, "gene": "manB", "product": "Phosphomannomutase", "db_xrefs": [ "COG:COG1109", "COG:G", "EC:5.4.2.8", "GO:0000287", "GO:0004615", "GO:0005975", "GO:0006166", "GO:0008973", "SO:0001217", "UniRef:UniRef50_P47299", "UniRef:UniRef90_P47299" ], "nt": "ATGGATAAACTTAGATTAGAAGTTGAAAGATGGTTAAATCATCCTAATGTTAATTGGGAGTTAAAACAACAAATTAAGGAGTTGAATGAATCAGAAATTCAAGAACTTTTTAGTTTGGAAAAACCTTTATTTGGCACTGCAGGTGTAAGAAACAAAATGGCACCAGGTTATCATGGTATGAATGTTTTTTCTTATGCCTATTTGACCCAAGGTTATGTTAAGTACATTGAATCCATCAATGAACCAAAGCGTCAACTACGGTTTTTAGTAGCACGTGATACAAGAAAAAATGGTGGTTTATTTTTAGAAACGGTTTGTGATGTAATTACATCTATGGGTCATTTGGCTTATGTGTTTGATGATAACCAGCCAGTTTCAACACCTCTAGTGTCCCATGTCATTTTTAAATATGGTTTTAGTGGAGGTATTAATATCACAGCTAGCCATAACCCTAAAGATGATAATGGTTTTAAGGTTTATGATCATACTGGTGCACAGCTTTTAGACACACAAACAAACCAATTGTTAAGTGATTTACCTTGTGTTACATCTATGCTAGATTTGGAATTACAACCAAATCCAAAGTTTGTCCATACTCTTGACAATGAAAAGGTTTATAAAAACTATTTCAGAGAGTTGAAAAAGGTGTTGGTTATTAACAACAACAATTTCAAAGACATTAAGGTAGTTTTTAGTGGGCTTAATGGGACTTCAGTTTGCTTAATGCAACGCTTTTTAAAGTACCTTGGTTATAGCAATATTATCAGTGTTGAGGAACAAAATTGGTTTGATGAGAATTTTGAAAATGCTCCTAACTTAAATCCAGAGTATAAAGATACATGGATATTAGCACAAAAATATGCTAAGAAAAATAATGCTAAGTTAATTATTATGGCAGACCCTGATGCTGATAGATTTGCAATTGCAGAGTTAAATAATAATCAATGA", "aa": "MDKLRLEVERWLNHPNVNWELKQQIKELNESEIQELFSLEKPLFGTAGVRNKMAPGYHGMNVFSYAYLTQGYVKYIESINEPKRQLRFLVARDTRKNGGLFLETVCDVITSMGHLAYVFDDNQPVSTPLVSHVIFKYGFSGGINITASHNPKDDNGFKVYDHTGAQLLDTQTNQLLSDLPCVTSMLDLELQPNPKFVHTLDNEKVYKNYFRELKKVLVINNNNFKDIKVVFSGLNGTSVCLMQRFLKYLGYSNIISVEEQNWFDENFENAPNLNPEYKDTWILAQKYAKKNNAKLIIMADPDADRFAIAELNNNQ", "aa_hexdigest": "bc52937199f95df1025bd05d86d79105", "start_type": "ATG", "rbs_motif": null, "genes": [ "manB" ], "truncated": "3-prime", "pseudogene": { "start": 61406, "stop": 62656, "observations": { "mutation": [ 62351 ], "stop": [ 62351 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47299", "query_cov": 1.0, "subject_cov": 0.5727272727272728, "identity": 1.0, "score": 640.0, "evalue": 1.42e-228, "gene_start": 1, "gene_end": 315, "reference_start": 1, "reference_end": 315, "reference_sequence": "MDKLRLEVERWLNHPNVNWELKQQIKELNESEIQELFSLEKPLFGTAGVRNKMAPGYHGMNVFSYAYLTQGYVKYIESINEPKRQLRFLVARDTRKNGGLFLETVCDVITSMGHLAYVFDDNQPVSTPLVSHVIFKYGFSGGINITASHNPKDDNGFKVYDHTGAQLLDTQTNQLLSDLPCVTSMLDLELQPNPKFVHTLDNEKVYKNYFRELKKVLVINNNNFKDIKVVFSGLNGTSVCLMQRFLKYLGYSNIISVEEQNWFDENFENAPNLNPEYKDTWILAQKYAKKNNAKLIIMADPDADRFAIAELNNNQWHYFSGNETGAITAYYKLNHKVFKSPYIVSTFVSTYLVNKIAKRYGAFVHRTNVGFKYIGQAINELSQTNELVVGFEEAIGLITSDKLNREKDAYQAAALLLEIARHCKEQNITLLDFYKRILSEFGEYFNLTISHPFKATATDWKEEIKALFNQLINANLTEVAGFKVVKVHLDKQTNILEFGFENGWVKFRFSGTEPKLKFYFDLTNGTREALEKQAKKIYKFFVNLLKLNKA" }, "paralog": false, "identity": 0.9975961538461539, "score": 841.0, "evalue": 2.72e-310, "description": "Internal stop codon at 62351. Nonsense mutation around 62351.", "psc": { "uniref90_id": "UniRef90_P47299", "gene": "manB", "product": "Phosphomannomutase", "ec_ids": [ "5.4.2.8" ], "uniref50_id": "UniRef50_P47299", "cog_id": "COG1109", "cog_category": "G", "go_ids": [ "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47299", "EC:5.4.2.8", "UniRef:UniRef50_P47299", "COG:COG1109", "COG:G", "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_P47299", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47299" ], "product": "Phosphomannomutase" } }, "id": "KKNAAMEMLJ_157", "locus": "GCA000027325_00117" }, { "type": "cds", "sequence": "contig_1", "start": 62459, "stop": 62785, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "TTGGTAAATAAGATTGCTAAAAGATATGGCGCTTTTGTGCATAGAACCAATGTTGGTTTTAAGTACATTGGTCAAGCAATTAATGAGTTATCACAAACAAACGAATTAGTTGTTGGTTTTGAAGAGGCAATTGGTTTAATAACTAGTGATAAATTAAACCGCGAGAAAGATGCTTATCAAGCTGCTGCATTATTGCTTGAGATTGCTAGACATTGCAAAGAACAAAACATCACGCTTTTAGATTTTTATAAAAGAATTCTTTCTGAGTTTGGTGAATATTTCAATTTAACAATATCTCATCCCTTTAAAGCTACTGCTACTGATTGA", "aa": "MVNKIAKRYGAFVHRTNVGFKYIGQAINELSQTNELVVGFEEAIGLITSDKLNREKDAYQAAALLLEIARHCKEQNITLLDFYKRILSEFGEYFNLTISHPFKATATD", "aa_hexdigest": "136ea89dfccaa978498ac34c51588023", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 83, "aa_cov": 0.7685185185185185, "hmm_cov": 0.9036144578313253, "evalue": 5.640199947108665e-14, "score": 47.37049865722656, "start": 1, "stop": 83 } ], "seq_stats": { "molecular_weight": 12285.895599999985, "isoelectric_point": 6.831103324890138 }, "id": "KKNAAMEMLJ_158", "locus": "GCA000027325_00118" }, { "type": "cds", "sequence": "contig_1", "start": 62840, "stop": 63058, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00408.26" ], "nt": "GTGGCTGGTTTTAAAGTAGTTAAAGTCCATCTTGATAAACAAACAAATATCTTAGAGTTTGGTTTTGAAAATGGCTGGGTTAAATTCCGCTTTTCAGGTACTGAACCTAAATTGAAATTTTACTTTGACCTAACTAATGGCACTAGAGAGGCTCTAGAAAAGCAAGCTAAGAAAATTTATAAATTCTTTGTAAATTTACTCAAACTCAACAAAGCTTAA", "aa": "MAGFKVVKVHLDKQTNILEFGFENGWVKFRFSGTEPKLKFYFDLTNGTREALEKQAKKIYKFFVNLLKLNKA", "aa_hexdigest": "9704d7a0affff9369fc6f9fdfe87e693", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00408.26", "name": "PGM_PMM_IV", "length": 54, "aa_cov": 0.75, "hmm_cov": 0.9629629629629629, "evalue": 2.7230216088192256e-08, "score": 28.900632858276367, "start": 7, "stop": 60 } ], "seq_stats": { "molecular_weight": 8461.833499999993, "isoelectric_point": 9.8733491897583 }, "id": "KKNAAMEMLJ_159", "locus": "GCA000027325_00119" }, { "type": "cds", "sequence": "contig_1", "start": 63035, "stop": 63556, "strand": "-", "frame": 2, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "COG:COG0250", "COG:K", "GO:0005829", "GO:0006353", "GO:0006355", "GO:0031564", "GO:0032784", "GO:0140673", "SO:0001217", "UniRef:UniRef50_P47300", "UniRef:UniRef90_P47300" ], "nt": "ATGCTTAAAGGGATATCAATCAATCCTAGCAAACGAATTATGCTAACAAATACTGCCATTATTGAAATGGACAGTGATAAATTTGATGAAAAATTTCAATATATCTTAAAACAAAAACAAGCCATTCAAAAACCAAAAGAGGATGAAGATTCAGAAATTGTTGATGCTGAAAAACTAAAAGAAGCTTTTAAAAAACTACAAAATAGTCAAGAACAAGATGAATGGAAAGAAAAAGCAACGATTATTCAAAGTGAGCAAACCAAACTTGATCCATCAGTATTAGTTCCTTTTTTGGGCAAATATGAAATTCTTGATACTGACAATAAAGTTGAACAACTCTTTGAATTTAGTGTTGGTAATTTAGTAGAGGTACATTTAACTGATACTATTCATGTTCAAGGACAGATAAAAGCACTTTATCAAGGTACAGTTAACAAAGCAGTTGTAGAGATAGAATTAACATCTAAAACCCAATTAATTAACTTACCTTTAGAAAATTTAAGCTTTGTTGAGTTTGAGTAA", "aa": "MLKGISINPSKRIMLTNTAIIEMDSDKFDEKFQYILKQKQAIQKPKEDEDSEIVDAEKLKEAFKKLQNSQEQDEWKEKATIIQSEQTKLDPSVLVPFLGKYEILDTDNKVEQLFEFSVGNLVEVHLTDTIHVQGQIKALYQGTVNKAVVEIELTSKTQLINLPLENLSFVEFE", "aa_hexdigest": "405919f1e35823e1132b4e0609bf5aca", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "3-prime", "pseudogene": { "start": 63035, "stop": 63856, "observations": { "mutation": [ 63664 ], "stop": [ 63664 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47300", "query_cov": 1.0, "subject_cov": 0.5474683544303798, "identity": 1.0, "score": 326.0, "evalue": 1.67e-110, "gene_start": 1, "gene_end": 173, "reference_start": 144, "reference_end": 316, "reference_sequence": "MQASELTPKWYVAPVSIKDEAVVKNLKAKIQALGFNHEIVDVKVLKEREVHEEVYSLKSGKLPRSLKNTTFNKWFVLDDYRYLRVKISEKNLLGRYIYIKMIYSEDAWRIVRNFPGITGIVGSSGRGALPIPLDEKDANNLEQMLKGISINPSKRIMLTNTAIIEMDSDKFDEKFQYILKQKQAIQKPKEDEDSEIVDAEKLKEAFKKLQNSQEQDEWKEKATIIQSEQTKLDPSVLVPFLGKYEILDTDNKVEQLFEFSVGNLVEVHLTDTIHVQGQIKALYQGTVNKAVVEIELTSKTQLINLPLENLSFVEFE" }, "paralog": false, "identity": 0.9963369963369964, "score": 531.0, "evalue": 6.37e-194, "description": "Internal stop codon at 63664. Nonsense mutation around 63664.", "psc": { "uniref90_id": "UniRef90_P47300", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_P47300", "cog_id": "COG0250", "cog_category": "K", "go_ids": [ "GO:0005829", "GO:0006353", "GO:0006355", "GO:0031564", "GO:0032784", "GO:0140673" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47300", "UniRef:UniRef50_P47300", "COG:COG0250", "COG:K", "GO:0005829", "GO:0006353", "GO:0006355", "GO:0031564", "GO:0032784", "GO:0140673" ] }, "pscc": { "uniref50_id": "UniRef50_P47300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47300" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "KKNAAMEMLJ_160", "locus": "GCA000027325_00120" }, { "type": "cds", "sequence": "contig_1", "start": 63662, "stop": 63985, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTAGTGAATTAACACCAAAGTGGTATGTAGCTCCTGTTAGTATTAAAGATGAAGCTGTTGTAAAAAATCTAAAAGCTAAAATTCAAGCTTTAGGATTTAATCATGAGATTGTTGATGTTAAAGTTCTAAAAGAAAGGGAAGTTCATGAAGAAGTTTATTCATTAAAATCAGGAAAACTTCCTCGTTCCTTAAAGAACACTACTTTTAACAAATGGTTTGTTCTTGATGATTACCGTTATCTTAGGGTAAAAATTAGTGAAAAAAATCTCCTTGGTAGATACATCTACATAAAGATGATTTATAGTGAAGATGCTTGA", "aa": "MQASELTPKWYVAPVSIKDEAVVKNLKAKIQALGFNHEIVDVKVLKEREVHEEVYSLKSGKLPRSLKNTTFNKWFVLDDYRYLRVKISEKNLLGRYIYIKMIYSEDA", "aa_hexdigest": "97401e671f986592ffc376eb3d641b5c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12579.512099999985, "isoelectric_point": 9.370753669738768 }, "id": "KKNAAMEMLJ_161", "locus": "GCA000027325_00121" }, { "type": "cds", "sequence": "contig_1", "start": 64145, "stop": 64360, "strand": "-", "frame": 2, "gene": "secE", "product": "preprotein translocase subunit SecE", "db_xrefs": [ "COG:COG0690", "COG:U", "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952", "SO:0001217", "UniRef:UniRef50_P47301", "UniRef:UniRef90_P47301" ], "nt": "ATGGAAAAAAAACTGCCTTTTAGCTTTAAAAAGAAGGAAAAGCTAACTGCTTATGATGATGCTTCAATTCATGAGTTACATAAACAGCTCAAACTTAGAACAGAAGCCAAGAAAAGTAAAGATAAGGAAAGAACTAAAGAAAAAGAAAAGCATGAAAGTTTAGCAAAGGAAAAGAAACCCAAGCTTCCTTTTAAAAAACGAATTGTTAATTTATGA", "aa": "MEKKLPFSFKKKEKLTAYDDASIHELHKQLKLRTEAKKSKDKERTKEKEKHESLAKEKKPKLPFKKRIVNL", "aa_hexdigest": "798ee827a94f4f5c9d633acf03b073b7", "start_type": "ATG", "rbs_motif": null, "genes": [ "secE" ], "truncated": "5-prime", "pseudogene": { "start": 63989, "stop": 64360, "observations": { "mutation": [ 64111, 64147 ], "stop": [ 64111, 64147 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47301", "query_cov": 1.0, "subject_cov": 0.5772357723577236, "identity": 1.0, "score": 112.0, "evalue": 9.56e-31, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MEKKLPFSFKKKEKLTAYDDASIHELHKQLKLRTEAKKSKDKERTKEKEKHESLAKEKKPKLPFKKRIVNLWFGVDKEINKIVWVKGRQLIIIFLLILLVSGLMVGIFFGINQLLITLGIFKN" }, "paralog": false, "identity": 0.983739837398374, "score": 210.0, "evalue": 7.21e-72, "description": "Internal stop codon at 64111, 64147. Nonsense mutation around 64111, 64147.", "psc": { "uniref90_id": "UniRef90_P47301", "gene": "secE", "product": "preprotein translocase subunit SecE", "uniref50_id": "UniRef50_P47301", "cog_id": "COG0690", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47301", "UniRef:UniRef50_P47301", "COG:COG0690", "COG:U", "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_P47301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47301" ], "product": "Uncharacterized protein MG055" } }, "id": "KKNAAMEMLJ_162", "locus": "GCA000027325_00122" }, { "type": "cds", "sequence": "contig_1", "start": 64668, "stop": 64910, "strand": "-", "frame": 1, "gene": null, "product": "MPN070 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75047", "UniRef:UniRef90_Q9ZB81" ], "nt": "ATGAAAGTATTTAAAAAACTCCATAACCTTAATGAGATTCTTTTGTTAATTAGTATCTTTTTTTTAGTTTGTATTATCTCTTTAGTAGGGATTGGTATCATCTTTGATTTAATTAGAAAAGCTAGTTTAAGTGCAATTAGAAGTGATCCTATTTTTTTTAATTTACGAGCAGTTTTAATTGTTTTAGGAGTTTTTGCTATCTGTTTTATGATCATCCAGTTAGTTATCAGCATAATGATTTGA", "aa": "MKVFKKLHNLNEILLLISIFFLVCIISLVGIGIIFDLIRKASLSAIRSDPIFFNLRAVLIVLGVFAICFMIIQLVISIMI", "aa_hexdigest": "72a53a11fe15a0e34b58945654560d0a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 64527, "stop": 64910, "observations": { "mutation": [ 64610, 64670 ], "stop": [ 64610, 64670 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZB81", "query_cov": 1.0, "subject_cov": 0.6299212598425197, "identity": 1.0, "score": 135.0, "evalue": 1.15e-39, "gene_start": 1, "gene_end": 80, "reference_start": 1, "reference_end": 80, "reference_sequence": "MKVFKKLHNLNEILLLISIFFLVCIISLVGIGIIFDLIRKASLSAIRSDPIFFNLRAVLIVLGVFAICFMIIQLVISIMIWKTINTACENIEPKFKKILHWSCFLPFGLLQLYCYQKIKLVKQADNL" }, "paralog": false, "identity": 0.984251968503937, "score": 241.0, "evalue": 1.16e-83, "description": "Internal stop codon at 64610, 64670. Nonsense mutation around 64610, 64670.", "psc": { "uniref90_id": "UniRef90_Q9ZB81", "product": "MPN070 family protein", "uniref50_id": "UniRef50_P75047", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZB81", "UniRef:UniRef50_P75047", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75047", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75047" ], "product": "Uncharacterized protein MG055.2 homolog" } }, "id": "KKNAAMEMLJ_163", "locus": "GCA000027325_00123" }, { "type": "cds", "sequence": "contig_1", "start": 64897, "stop": 64998, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTGGTGTTAAGTTAAAAGATGCATGCAATTATTTAGCTAAAAAAATGCATTTAAAATCAAGTATGCTCTATACACTTTTCCATGAAAGTATTTAA", "aa": "MDIGVKLKDACNYLAKKMHLKSSMLYTLFHESI", "aa_hexdigest": "52022b2b22d050a7493e856737549861", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3829.575, "isoelectric_point": 8.93288440704346 }, "id": "KKNAAMEMLJ_164", "locus": "GCA000027325_00124" }, { "type": "cds", "sequence": "contig_1", "start": 65131, "stop": 65730, "strand": "-", "frame": 3, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677", "KEGG:K07056", "SO:0001217", "UniRef:UniRef50_P47302", "UniRef:UniRef90_P47302" ], "nt": "ATGAAAACACTTAAAGTAGTTGCTACACCAATTGGTAATATTCAAGAGATAAGTGAAAGAGCTAAAAAAGCGTTACAAGATTGTGAAGTGTTATTCTGTGAAGATAGTAGAGTAACAAGAAAAATGCTGGATTTACTCAACATTGATTGCAAGCAAAAAAAGTTTGTTATTAACAACAGTTTTAAAGAAAAACAGAATTTAACTTTTGCTGAGGAATTTATAACTAACTTTAAGTGTTGTCTTGTTAGTGATGCAGGTTATCCTAGTTTATCTGATCCTGGTAATGAAATGATTAATTGGATCATATCAAAAAATAAGGAGATAAGAATCGAAGTTATTAATGGTCCTAGTGCATTAATGTGTGGATTGATCACTAGTGGTTTTAAAACAACACCACTTTTGTTTTTAGGTTTTTTAAGTCACAAACAAAATCAGCTCAAAAATTATTTAAGTACCTATCAAAATCAGAAAAGCACTATCGTTTTTTTTGAAGCAGTGCATAGGTTAGAAAATACTTTAGAAACTGTGAAAAATGTTTTTAAAAACAATGACGTTTTTATAGGAAGAGAATTAACTAAGTTACATGAATCACATTATTGA", "aa": "MKTLKVVATPIGNIQEISERAKKALQDCEVLFCEDSRVTRKMLDLLNIDCKQKKFVINNSFKEKQNLTFAEEFITNFKCCLVSDAGYPSLSDPGNEMINWIISKNKEIRIEVINGPSALMCGLITSGFKTTPLLFLGFLSHKQNQLKNYLSTYQNQKSTIVFFEAVHRLENTLETVKNVFKNNDVFIGRELTKLHESHY", "aa_hexdigest": "ea88c608b672882c3bed7c6d2833ae44", "start_type": "ATG", "rbs_motif": null, "genes": [ "rsmI" ], "truncated": "5-prime", "pseudogene": { "start": 64897, "stop": 65730, "observations": { "mutation": [ 65133 ], "stop": [ 65133 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47302", "query_cov": 1.0, "subject_cov": 0.7184115523465704, "identity": 1.0, "score": 400.0, "evalue": 3.72e-140, "gene_start": 1, "gene_end": 199, "reference_start": 1, "reference_end": 199, "reference_sequence": "MKTLKVVATPIGNIQEISERAKKALQDCEVLFCEDSRVTRKMLDLLNIDCKQKKFVINNSFKEKQNLTFAEEFITNFKCCLVSDAGYPSLSDPGNEMINWIISKNKEIRIEVINGPSALMCGLITSGFKTTPLLFLGFLSHKQNQLKNYLSTYQNQKSTIVFFEAVHRLENTLETVKNVFKNNDVFIGRELTKLHESHYWFNTSENTLPDITLKGEFVIVIDNQNINHQTLSSNQYLVYEIKKLMDIGVKLKDACNYLAKKMHLKSSMLYTLFHESI" }, "paralog": false, "identity": 0.9963898916967509, "score": 552.0, "evalue": 1.75e-202, "description": "Internal stop codon at 65133. Nonsense mutation around 65133.", "psc": { "uniref90_id": "UniRef90_P47302", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_P47302", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47302", "EC:2.1.1.198", "UniRef:UniRef50_P47302", "COG:COG0313", "COG:J", "KEGG:K07056", "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677" ] }, "pscc": { "uniref50_id": "UniRef50_P47302", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47302" ], "product": "Ribosomal RNA small subunit methyltransferase I" } }, "id": "KKNAAMEMLJ_165", "locus": "GCA000027325_00125" }, { "type": "cds", "sequence": "contig_1", "start": 65865, "stop": 66248, "strand": "-", "frame": 1, "gene": "rnmV", "product": "ribonuclease M5", "db_xrefs": [ "COG:COG1658", "COG:J", "EC:3.1.26.8", "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872", "KEGG:K05985", "SO:0001217", "UniRef:UniRef50_P75045", "UniRef:UniRef90_P47303" ], "nt": "ATGGATCAAAAAGCACGTATAAAAATTGATGGGGTAATTGTTTGTGAGGGTAAAACTGACCAAGCTAAATTACAGCAAATTTTTGATGTAGATGTAATTACAACAAATGGTTCAGCTTTAAAAAAAGAAACTATTAACCTAATTAAAAAAATCAGTGAAAAGCAAACTGTTATCTTGTTATTAGATCCAGATCAACAAGGCAAAAAGATTAGAAGTAAATTAGAACAACATCTAACAAACTATTACAACTGTTATGTTGATATGAATGCAAGGTTAAAAAACGCTAAAAAAGCTGGTATTGCTGAAATGGAAACTAGTGCTTTAATTGCTGCTTTAAATAATCGTGTTGCAATCACAAAAAATGCTAATCAAAGCATATTATGA", "aa": "MDQKARIKIDGVIVCEGKTDQAKLQQIFDVDVITTNGSALKKETINLIKKISEKQTVILLLDPDQQGKKIRSKLEQHLTNYYNCYVDMNARLKNAKKAGIAEMETSALIAALNNRVAITKNANQSIL", "aa_hexdigest": "cae419be1a45db980494db4726f97918", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnmV" ], "truncated": "5-prime", "pseudogene": { "start": 65712, "stop": 66248, "observations": { "mutation": [ 65729, 65867 ], "stop": [ 65729, 65867 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47303", "query_cov": 1.0, "subject_cov": 0.7134831460674157, "identity": 1.0, "score": 243.0, "evalue": 9.14e-81, "gene_start": 1, "gene_end": 127, "reference_start": 1, "reference_end": 127, "reference_sequence": "MDQKARIKIDGVIVCEGKTDQAKLQQIFDVDVITTNGSALKKETINLIKKISEKQTVILLLDPDQQGKKIRSKLEQHLTNYYNCYVDMNARLKNAKKAGIAEMETSALIAALNNRVAITKNANQSILWDQYLSLKLNDKKKRLLLCNNLNLPYFNHKQLFKKLNLLNLTFQDVWKHLK" }, "paralog": false, "identity": 0.9887640449438202, "score": 342.0, "evalue": 2.79e-122, "description": "Internal stop codon at 65729, 65867. Nonsense mutation around 65729, 65867.", "psc": { "uniref90_id": "UniRef90_P47303", "gene": "rnmV", "product": "ribonuclease M5", "ec_ids": [ "3.1.26.8" ], "uniref50_id": "UniRef50_P75045", "cog_id": "COG1658", "cog_category": "J", "kegg_orthology_id": "K05985", "go_ids": [ "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47303", "EC:3.1.26.8", "UniRef:UniRef50_P75045", "COG:COG1658", "COG:J", "KEGG:K05985", "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75045", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75045" ], "product": "Ribonuclease M5" } }, "id": "KKNAAMEMLJ_166", "locus": "GCA000027325_00126" }, { "type": "cds", "sequence": "contig_1", "start": 66227, "stop": 67318, "strand": "-", "frame": 2, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "GO:0000287", "GO:0002189", "GO:0004749", "GO:0005524", "GO:0005737", "GO:0006015", "GO:0006164", "GO:0009156", "GO:0016301", "KEGG:K00948", "SO:0001217", "UniRef:UniRef50_P47304", "UniRef:UniRef90_P47304" ], "nt": "ATGGCAAAATTAAAGTGGAAATTTGATTGGCAAAACCTAAAAAGAAATTTGACAAACGTGAAACTATTAAAAAGAAAACAATCCGACGCGAGCTTGAAGCTGAGTATCGATAATAAAAAGCACGTTATTTTTAGTTTATCTAAAAGCAAAACACTGGTTGAAAATATCTGCAAAAAACTAAATATTTCAGAGGGCAAAATGGTTTGCGAACACTTTGCTGATGGTGAAACTTATATCCGTTTTGATGAATCAGTTCGTAACAAAGATATCTATATTTTTCAATCAACCTGTCCTAATGTTAACGATAGCTTAATGGAACTTTTAATTGCTATTGATGCATTGAAAAGAGGTAGTGCTAAAAGTATTACTGCCATTCTACCCTATTATGGATATGCAAGACAAGATAGAAAAACAAAAGGAAGAGAACCAATTACCAGTAAATTGATTGCTGATATGTTAACAAAAGCAGGTGCTAACAGGGTTGTTCTAACTGACATTCATAGTGATCAAACCCAAGGTTTTTTTGATATTCCCGTTGATTCTTTAAGAACTTATCACATCTTTCTTTTTAGAGTTATAGAACTACTTGGTAAAAAAGACTTGGTGGTTGTTTCCCCTGATTATGGTGGGGTTAAAAGAGCAAGGTTAATTGCAAATACACTAGAACTACCATTAGCCATTATTGATAAAAGAAGACCATCTCATAATGTTGCTGAATCAATTAATGTTTTAGGTGAAGTGAAAAATAAAAACTGTTTAATAGTTGATGACATGATAGATACTGGTGGTACAGTAATTGCAGCAGCCAAGCTATTACAAAAAGAACAAGCTAAAAAAGTGTGTGTAATGGCAACTCATGGTTTGTTTAACAATGATGCAGAACAAAAGTTTATGGAAGCATTTGATCAAAAACTAATTGATTTCTTGTTTGTATCAAACTCTATTCCTCAATATAAGTTTAAAGCTGTAAAGCAGTTTGAAGTAGTTGATCTAGCATCTTTATATGAAGAGGTTGTTCTGTGTTACGCTAACAGCTTATCAGTTTCTGCAATATATGAACGTCACATTGAATGGATCAAAAAGCACGTATAA", "aa": "MAKLKWKFDWQNLKRNLTNVKLLKRKQSDASLKLSIDNKKHVIFSLSKSKTLVENICKKLNISEGKMVCEHFADGETYIRFDESVRNKDIYIFQSTCPNVNDSLMELLIAIDALKRGSAKSITAILPYYGYARQDRKTKGREPITSKLIADMLTKAGANRVVLTDIHSDQTQGFFDIPVDSLRTYHIFLFRVIELLGKKDLVVVSPDYGGVKRARLIANTLELPLAIIDKRRPSHNVAESINVLGEVKNKNCLIVDDMIDTGGTVIAAAKLLQKEQAKKVCVMATHGLFNNDAEQKFMEAFDQKLIDFLFVSNSIPQYKFKAVKQFEVVDLASLYEEVVLCYANSLSVSAIYERHIEWIKKHV", "aa_hexdigest": "dd7d383aef5d31152815e2ebc3691c66", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47304", "query_cov": 0.9146005509641874, "subject_cov": 1.0, "identity": 0.997, "score": 650.0, "evalue": 5.34e-235, "valid": true, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_P47304", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948", "go_ids": [ "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ] }, "pscc": { "uniref50_id": "UniRef50_P47304", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47304" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prs" ], "id": "KKNAAMEMLJ_167", "locus": "GCA000027325_00127" }, { "type": "cds", "sequence": "contig_1", "start": 67293, "stop": 67643, "strand": "-", "frame": 1, "gene": "smpB", "product": "SsrA-binding protein", "db_xrefs": [ "COG:COG0691", "COG:O", "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929", "KEGG:K03664", "SO:0001217", "UniRef:UniRef50_P75043", "UniRef:UniRef90_P47305" ], "nt": "ATGTTAATTCTTGTTAACAATCCTAAGGCTAAATATGACTATCATTTAATGGAATCTTATTGTGCTGGAATAGTTTTAAAAGGAAGTGAAGTTAAAGCTTTAAGTTTAGGTCAAGGTAGTTTAAAAGAAGCTTATGTTTTTGTTAAAAACAATGAGCTTTTTTTAGAACAGTTCACTATTCCACCTTATAGTTTTGCAGGTCCATTAAATCACGCTTCAGATAGAATTAAGAAACTTTTATTAAATAAACATGAAATTAAACAAATTATCAATAAAAAACAACAACAATCTTTATCTGTAATCCCAAGTAAAGTATTCTTTAGAAATGGCAAAATTAAAGTGGAAATTTGA", "aa": "MLILVNNPKAKYDYHLMESYCAGIVLKGSEVKALSLGQGSLKEAYVFVKNNELFLEQFTIPPYSFAGPLNHASDRIKKLLLNKHEIKQIINKKQQQSLSVIPSKVFFRNGKIKVEI", "aa_hexdigest": "1c2fff54eab8bedf7143b6f1d9747546", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47305", "query_cov": 1.0, "subject_cov": 0.8, "identity": 1.0, "score": 228.0, "evalue": 2.41e-75, "valid": true, "gene": "smpB", "product": "SsrA-binding protein", "uniref50_id": "UniRef50_P75043", "cog_id": "COG0691", "cog_category": "O", "kegg_orthology_id": "K03664", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929" ] }, "pscc": { "uniref50_id": "UniRef50_P75043", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75043" ], "product": "SsrA-binding protein" }, "genes": [ "smpB" ], "id": "KKNAAMEMLJ_168", "locus": "GCA000027325_00128" }, { "type": "cds", "sequence": "contig_1", "start": 67650, "stop": 67916, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00535.33" ], "nt": "ATGAAATTATCTGTAATTATACCTACTTACAATTGTGCATCATTTATTGAAAAAGCAATTAATTCAATTGTTAAAAATAGACCTAATGATTTGGAAATAGAAGTTTTAATTATTGATGATGGATCAATTGACAATACTAACAAAGTTATTAAGAAAATTCAAGACCAAATTAATAATTTAACTTTGCAGTATTTTTACAAAAGTAATGGTAACTGGGGTAGTGTTATTAATTATGTTAGAAACAATAAACTAGCAAAAGGGGAATGA", "aa": "MKLSVIIPTYNCASFIEKAINSIVKNRPNDLEIEVLIIDDGSIDNTNKVIKKIQDQINNLTLQYFYKSNGNWGSVINYVRNNKLAKGE", "aa_hexdigest": "26cec3462c71ae6ebe902523f5cd7737", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00535.33", "name": "Glycos_transf_2", "length": 84, "aa_cov": 0.9545454545454546, "hmm_cov": 0.9404761904761905, "evalue": 3.1655108728951803e-17, "score": 58.06611251831055, "start": 4, "stop": 87 } ], "seq_stats": { "molecular_weight": 10017.366199999995, "isoelectric_point": 8.732774543762208 }, "id": "KKNAAMEMLJ_169", "locus": "GCA000027325_00129" }, { "type": "cds", "sequence": "contig_1", "start": 67936, "stop": 68094, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTTTTTCAAAAAAAACAATTTCTATTTTTCAAAAATATGCCCAAAAACAAAGATATGATGCGATTATTTTTGACTACTATAAATGCTGAAAAAAGTTTTTGTGAAAAATTCCTACCTATGCAAGGTTTAGAAAAGAAATTAAAGGTGAATTGA", "aa": "MTFFQKKQFLFFKNMPKNKDMMRLFLTTINAEKSFCEKFLPMQGLEKKLKVN", "aa_hexdigest": "941594f13135f7075475b6bd2801b7ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6339.647499999999, "isoelectric_point": 10.01376132965088 }, "id": "KKNAAMEMLJ_170", "locus": "GCA000027325_00130" }, { "type": "cds", "sequence": "contig_1", "start": 68091, "stop": 68303, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAACAAACACCTTTTTGTATTCCCTTAGCTAAGTTTTTTAAAAATGAGGTTTTCTATCAACTTCCTAAACTAAGAGAAAATGTTGGTTTTCAAGACGCTATTTATACGATGCATGCATTACAAATTGCAAATAATGTTTTCCATGTTTCTAAAGCTGGAGGATATTACTTTTTTAAAAGGGTAGGTAACTCTATGAGTATCCCTTGA", "aa": "MKKQTPFCIPLAKFFKNEVFYQLPKLRENVGFQDAIYTMHALQIANNVFHVSKAGGYYFFKRVGNSMSIP", "aa_hexdigest": "21a3cece4e3be010d522798d65e95ecf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8121.4417, "isoelectric_point": 9.779418754577634 }, "id": "KKNAAMEMLJ_171", "locus": "GCA000027325_00131" }, { "type": "cds", "sequence": "contig_1", "start": 68591, "stop": 69037, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTCTTATTAACAGCTAACATAGTGGTATTGCTTATAATCATCATGTTTGCAGCAACACTTGGTATTGGTAGTGCAGCTGGGTTTGCTTTAATTAGTATCTTTTCATTTATCGGTGGTGCATTTGCTTGGAGTTTATCAAGTTCAAATTTAATCCTGCCATATGAATTTAAAGATTACAAGAAGAATGAACTTCCTATTCTTTTTGGATTTTGTTGGGGCTTTGGTTATATTGCTTACACCTTATTTGACATTACCCAATCAGTATTTCTCCAAGCGCCTGTTTTAATTCAAGGAAAAGGTACAAGTATTTTACCAGGGGTAATTGTAAGTATTGTTTTCTTCTTAGGACTTATTGCACTAGCAAACCTAATTGTTAAGTTCTTTCCTGCTTGTTGGATTAAAGATGGTGATCAATTGGTTCAGGAAATGACAAGAAAATGA", "aa": "MHFLLTANIVVLLIIIMFAATLGIGSAAGFALISIFSFIGGAFAWSLSSSNLILPYEFKDYKKNELPILFGFCWGFGYIAYTLFDITQSVFLQAPVLIQGKGTSILPGVIVSIVFFLGLIALANLIVKFFPACWIKDGDQLVQEMTRK", "aa_hexdigest": "f78a76e8d077db6d7b3fa4e0b2926108", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16253.248899999984, "isoelectric_point": 7.752804374694825 }, "id": "KKNAAMEMLJ_172", "locus": "GCA000027325_00132" }, { "type": "cds", "sequence": "contig_1", "start": 69269, "stop": 69979, "strand": "-", "frame": 2, "gene": null, "product": "Major facilitator superfamily transporter MG061", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47307", "UniRef:UniRef90_P47307" ], "nt": "TTGAGAGCAGTAGGTTCTATTCTTTGTGGAGTTGTTGTTTTGAAATTTGGTTATCGTTATGCGGTTTTAATCATGATGGGAATCATGTGTGTTTGTTTTCCCTTTCTTATTATAGGAGACCCTTTGAATGGTCATAATCAATTAACTTTATTAAGACCTTTATCAGATTCAGTAAAAACTCAATTAAGTTCACTAAGTAGCAATTTACAAGTGGGTCAATTACTTGGACCGGTGATGATCAATGGTAAAACGATGCTTGCAGATGGAACAAGTGTTGAACTTATTAAGGGTTTGGATGGTAATAGTATTGGTACAGCTGCATCAATTACTGGTTATGCATTGTTTATTATCTTTAGAAGCACAATTGCTATTGGTGGAACTACCTTAGTAGTTTATACTCAACCAGCAATTGCTAATCTTTCTTCAAACAGAAAAAAATCAATCCTATCTAATGCTAACCTTTGGGGTTTTAACATTGGGATTGCAGTAGTTTTCACACCGTTTTTATTTGAACAAGTTCAACAAGTAGCCTCTCAATACTGGGTTTACATTATGACTGTGATGATTTTGGTTGTGTTTGCTAACTTATGTTTATTTTTATGGTTTGAAAGCAAGATAGATCACATCTTTCCTCAAAAGCAAACTAAGGAAAATATGAGTTTAACTACTCAACCAAAATCCATAGATATCTTAAAGAATAAAACAACATGA", "aa": "MRAVGSILCGVVVLKFGYRYAVLIMMGIMCVCFPFLIIGDPLNGHNQLTLLRPLSDSVKTQLSSLSSNLQVGQLLGPVMINGKTMLADGTSVELIKGLDGNSIGTAASITGYALFIIFRSTIAIGGTTLVVYTQPAIANLSSNRKKSILSNANLWGFNIGIAVVFTPFLFEQVQQVASQYWVYIMTVMILVVFANLCLFLWFESKIDHIFPQKQTKENMSLTTQPKSIDILKNKTT", "aa_hexdigest": "5040f8586b191b71c17a1f3bf0093ea0", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 68966, "stop": 70225, "observations": { "mutation": [ 69040, 69112, 69208, 69271, 69994 ], "stop": [ 69040, 69112, 69208, 69271, 69994 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47307", "query_cov": 1.0, "subject_cov": 0.41622574955908287, "identity": 0.996, "score": 451.0, "evalue": 5.4e-155, "gene_start": 1, "gene_end": 236, "reference_start": 83, "reference_end": 318, "reference_sequence": "MENKSQKKVSDLKLITLWIIVIFGYLLFVVEWFVIDRISGKPTGILTQSTTTLPQYSGWLSSFFTENAGQIATSSTNWTITLLRAVGSILCGVVVLKFGYRYAVLIMMGIMCVCFPFLIIGDPLNGHNQLTLLRPLSDSVKTQLSSLSSNLQVGQLLGPVMINGKTMLADGTSVELIKGLDGNSIGTAASITGYALFIIFRSTIAIGGTTLVVYTQPAIANLSSNRKKSILSNANLWGFNIGIAVVFTPFLFEQVQQVASQYWVYIMTVMILVVFANLCLFLWFESKIDHIFPQKQTKENMSLTTQPKSIDILKNKTTWKLIGVYGIVLILIVNPLTPAWFSILQTVSPSGSNGLISTGVYTTGLATLAIFWVIGYALGFVVFSPFNKTIYDKKRWMHFLLTANIVVLLIIIMFAATLGIGSAAGFALISIFSFIGGAFAWSLSSSNLILPYEFKDYKKNELPILFGFCWGFGYIAYTLFDITQSVFLQAPVLIQGKGTSILPGVIVSIVFFLGLIALANLIVKFFPACWIKDGDQLVQEMTRKWKLNEWQFVIANKQKNRYSELLK" }, "paralog": false, "identity": 0.9856801909307876, "score": 796.0, "evalue": 1.43e-293, "description": "Internal stop codon at 69040, 69112, 69208, 69271, 69994. Nonsense mutation around 69040, 69112, 69208, 69271, 69994.", "psc": { "uniref90_id": "UniRef90_P47307", "product": "Major facilitator superfamily transporter MG061", "uniref50_id": "UniRef50_P47307", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47307", "UniRef:UniRef50_P47307", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47307", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47307" ], "product": "Major facilitator superfamily transporter MG061" } }, "id": "KKNAAMEMLJ_173", "locus": "GCA000027325_00133" }, { "type": "cds", "sequence": "contig_1", "start": 69992, "stop": 70225, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATAAAAGTCAAAAAAAAGTAAGTGATCTGAAACTTATTACCCTGTGGATCATAGTAATTTTTGGGTATCTATTGTTTGTAGTTGAATGGTTTGTAATTGATCGAATTAGTGGTAAACCAACTGGTATACTTACTCAATCAACCACCACTTTACCACAGTACAGTGGTTGGTTAAGTTCTTTCTTTACTGAAAATGCTGGGCAAATAGCCACATCTTCCACCAACTGA", "aa": "MENKSQKKVSDLKLITLWIIVIFGYLLFVVEWFVIDRISGKPTGILTQSTTTLPQYSGWLSSFFTENAGQIATSSTN", "aa_hexdigest": "9f8e71f0f49ac5cd20d65e72a80bf55c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8660.895099999994, "isoelectric_point": 8.06423740386963 }, "id": "KKNAAMEMLJ_174", "locus": "GCA000027325_00134" }, { "type": "tRNA", "sequence": "contig_1", "start": 70393, "stop": 70481, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 76.9, "nt": "GGGAGCGTACTCAAGTGGTTAAGAGGACACCCTGCTAAGGTGTTAGATCGTTCTACGGTGCGTGGGTTCGAATCCCACCGCTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 70446, 70448 ], "id": "KKNAAMEMLJ_34", "locus": "GCA000027325_00135" }, { "type": "cds", "sequence": "contig_1", "start": 70530, "stop": 70796, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00359.27" ], "nt": "ATGTTTAAAAATTTATTAAGACCATCCCTATTTTTTAACTGGAGCCAAAAAACATTTAAAAATAAATTTTCGTTTTTAAAACAAGCAGCAAATGCATTACAAAAACAGGCTGTTATCAATGATAACAATGTTGCATTTGAAGCTTTAAAAAAGCGTGAAGAAGAGATTACAACTGGAATTATTACTAGTTTAGCTTTACCTCACTTACAAAGTCAAAGTGTTATAGAACCTTTTGTTGCTGTATTTAAGGTTAAAAACTTAGATTGA", "aa": "MFKNLLRPSLFFNWSQKTFKNKFSFLKQAANALQKQAVINDNNVAFEALKKREEEITTGIITSLALPHLQSQSVIEPFVAVFKVKNLD", "aa_hexdigest": "69befee4b3c9dac3ce986b5e98e60c3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00359.27", "name": "PTS_EIIA_2", "length": 71, "aa_cov": 0.8068181818181818, "hmm_cov": 1.0, "evalue": 1.275707162267965e-14, "score": 49.511775970458984, "start": 16, "stop": 86 } ], "seq_stats": { "molecular_weight": 10084.609399999996, "isoelectric_point": 9.821838951110838 }, "id": "KKNAAMEMLJ_175", "locus": "GCA000027325_00136" }, { "type": "cds", "sequence": "contig_1", "start": 70824, "stop": 72572, "strand": "+", "frame": 3, "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "db_xrefs": [ "COG:COG1762", "COG:GT", "GO:0005351", "GO:0005886", "GO:0009401", "GO:0016301", "GO:0022877", "GO:0090563", "SO:0001217", "UniRef:UniRef50_P47308", "UniRef:UniRef90_P47308" ], "nt": "TTGATATTTTTAATTGGTGTTCTTGTTGACAAAACCAATTTGCATCTTGATTTCATTAGTAACTTTTCCAAGTTAATGTTGAATGAAACATTTGCAAGTAAGGTTTTAAATGTCACTAGCTATAACGGCTTGATTAAACTAATTGATCTTTTTAACCAACAAAAAGTGCAAGACCAACCAGCTGTTGAAACAAAAAAAGAGTATGACTTTGTTGCTGTAACAGCATGCCCAACTGGCATTGCCCATACTTTCATGGCTAAAGAAGCGTTGGAAGCATTTGCAAAAAAGCATAACTTATATGTAAAAGTTGAAACTCAAGGTACAGATGGGATACAAAATCAACTTACAAGTGACGATATTAATAACGCTAAAGGTGTTATTCTTGCTTGTGATCGTTTAATTGATTTCTCTAGGTTTTATGCAAATAAGAATGTGATTGAAGTGTCAACTACTAAAGCCATTAAAAAACCTGATGAGGTGTATGAATTAATTAAAAACCAAAAGGGTAAACAGCTTGCAAATAGTGCTAAACCAACTAATCAAACCCAATTAGCTGAAAGTGAAGGGGAATTCAACTTTAATAATTTTCACAAGCGGATTTATCGTGCCATTTTAAGTGGTGTTTCTTACATGCTACCATTCGTTGTTTTTGGTGGGATATTAATTGCACTCTCTTTTCTAATTGATATAAATAACGCTAATAATGCTGGTGGTAACTTTGGCACAATTAATCCTGTTGCTAACTGGTTAAATAAGCTAGGAGGAATTTCCTTTAGTTTAATTGTTCCTATCTTATCTGCATACATTGCATATGCATTGGTATCAAGACAGGGATTATTACCTGGTTTTGTTGTTGGTTTAATCTCTTCAGGACAATTTTTATTAAATATTGTTCTGACCAATGGAACAATTGAATGGTTAGCACCATCACAAGTATCAAGTGGCTTTTTTGGTGCTATCTTTGGAGGACTTTTGAGTGCTTGCTTAATAATTGTTCAACAAAACTACATCTATAAAAAATTACCTCAATCTTTGCAGGGAATTAAAAACATCTTGTTTATTCCCTTGTTTGGTACTTTATTCACTGCTGGTTTATTTTGGGTTATTAACATTCCTTTAATTTATCTAAACTATGGATTAAGTCTGTTTTTAAATATTATGAACAGCCCTATCCTAGCACCTTTACTTGGTTTTGTAATTGGGTTGATGATGTGCTTTGATTTAGGGGGGCCAATTAATAAAGCAGCTTATGTTTTTGGTGTTGTTTCTTTACAAAATCAAAATGCAGGAACAATTTCGATGGCTGCAGCTATGCTATCAGGGATGGTACCTCCTTTATCAATTGCTTTGGCAGCTTCCATTCGAAAGAGCTGCTTTGATAAACAGGAATTACCTGCAGCTTATGCTTGTTATCTGATGGGATTGAGTTTTATTAGTGAAGGTGCTATCCCATTTGTTGTTAAAAAACCTAAGGTGATGTTAACTGCTAACTTAATTGCTGGAGCAATTTGTGGAGCATTAACAGGAGCATTTGCCTTATCAATTCGTGCTCCTCATGGCGGTGTTTTTGTGTTTGCACTTTTAAAAACTACTTTACAAGGGATTGAAGGAGCTACATTACAAACTGGAGTTGGCATTGGTTTGGCATTGGTTTGTTTAATAATTAGTATGATAGTTGGTAGTAGTATTATCATTGGCTATGACTTGATTGCAAAACATAACCAAAGAAAGCAAAATCTGAATAGTTAA", "aa": "MIFLIGVLVDKTNLHLDFISNFSKLMLNETFASKVLNVTSYNGLIKLIDLFNQQKVQDQPAVETKKEYDFVAVTACPTGIAHTFMAKEALEAFAKKHNLYVKVETQGTDGIQNQLTSDDINNAKGVILACDRLIDFSRFYANKNVIEVSTTKAIKKPDEVYELIKNQKGKQLANSAKPTNQTQLAESEGEFNFNNFHKRIYRAILSGVSYMLPFVVFGGILIALSFLIDINNANNAGGNFGTINPVANWLNKLGGISFSLIVPILSAYIAYALVSRQGLLPGFVVGLISSGQFLLNIVLTNGTIEWLAPSQVSSGFFGAIFGGLLSACLIIVQQNYIYKKLPQSLQGIKNILFIPLFGTLFTAGLFWVINIPLIYLNYGLSLFLNIMNSPILAPLLGFVIGLMMCFDLGGPINKAAYVFGVVSLQNQNAGTISMAAAMLSGMVPPLSIALAASIRKSCFDKQELPAAYACYLMGLSFISEGAIPFVVKKPKVMLTANLIAGAICGALTGAFALSIRAPHGGVFVFALLKTTLQGIEGATLQTGVGIGLALVCLIISMIVGSSIIIGYDLIAKHNQRKQNLNS", "aa_hexdigest": "e06fc9f177bc2ee2338f14b29aab25d2", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47308", "query_cov": 1.0, "subject_cov": 0.8558823529411764, "identity": 0.998, "score": 1108.0, "evalue": 0.0, "valid": true, "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "uniref50_id": "UniRef50_P47308", "cog_id": "COG1762", "cog_category": "GT", "go_ids": [ "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47308", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47308" ], "product": "PTS system fructose-specific EIIABC component" }, "genes": [ "fruA" ], "id": "KKNAAMEMLJ_176", "locus": "GCA000027325_00137" }, { "type": "cds", "sequence": "contig_1", "start": 72667, "stop": 73434, "strand": "+", "frame": 1, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "db_xrefs": [ "COG:COG1105", "COG:G", "EC:2.7.1.56", "GO:0005524", "GO:0005829", "GO:0008443", "GO:0008662", "SO:0001217", "UniRef:UniRef50_Q49396", "UniRef:UniRef90_Q49396" ], "nt": "ATGAAATCACTTGGTTTTGATCCAACTGTCATTACTTTTTTGGGACAACCCACTAAAAACTTATTTTTAGAGTTGGTAAAACCTTATGATCTAAATATAGTTAGCTTCATTTCTGAAACTAAAACAAGAATTAACCTTAAGTTATTAAAAGATGAAAAAACTACTGAAATTAATGATTTAAGTCCTTTAATAACAGATGCTAATCTAACTGAATTGTTAACTTTTTTAAAAGCTAATGTTAAGAATAATGATTTGGTTATCATCAACGGAAGATTTAAATTTGAAGCTTTAGAAAAAGTTCTAAACTTGGTCTTTACATTAACAGAAAATGTGGTTATAGATGTTGATGAAAGCAAAATGTTAACGCTTTTAAATCAGTCTAAACCACTAGTTATGAAACCTAACATTGATGAGTTTCAAACTATGATTAATACTTTTTTTCACGATCAACAAAGCTTAATAGCAGCAATTAAAAAATTTCATTACTGTAAGCTCTTATTATTATCTGATGGTGACAAAGGAGCTTATCTTTTTGATCAGAATAAGTTATTGTTTGTAAGTTCTATCACTCCTAAACAAGTAGTTAGCACCACAGGAGCAGGTGATACTTTGTTGGCAGTTTTTTTAGCAAATTTGATTCTAAAGGTAGATTTAAAAACTGCTTTGATTAAAGCAACTAACTATGCAAGTGCAACAATTAGTAAGTTAGGTGTTGTTGATAGTAAAGACAAAATTAGTGTTATAACCCCAAAAAGTTACTATTTATAA", "aa": "MKSLGFDPTVITFLGQPTKNLFLELVKPYDLNIVSFISETKTRINLKLLKDEKTTEINDLSPLITDANLTELLTFLKANVKNNDLVIINGRFKFEALEKVLNLVFTLTENVVIDVDESKMLTLLNQSKPLVMKPNIDEFQTMINTFFHDQQSLIAAIKKFHYCKLLLLSDGDKGAYLFDQNKLLFVSSITPKQVVSTTGAGDTLLAVFLANLILKVDLKTALIKATNYASATISKLGVVDSKDKISVITPKSYYL", "aa_hexdigest": "0d21cfdaeeac6761160d13a7af20f43d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49396", "query_cov": 1.0, "subject_cov": 0.8415841584158416, "identity": 1.0, "score": 486.0, "evalue": 9.74e-173, "valid": true, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "ec_ids": [ "2.7.1.56" ], "uniref50_id": "UniRef50_Q49396", "cog_id": "COG1105", "cog_category": "G", "go_ids": [ "GO:0005829", "GO:0008662", "GO:0005524", "GO:0008443" ] }, "pscc": { "uniref50_id": "UniRef50_Q49396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49396" ], "product": "Putative 1-phosphofructokinase" }, "genes": [ "fruK" ], "id": "KKNAAMEMLJ_177", "locus": "GCA000027325_00138" }, { "type": "cds", "sequence": "contig_1", "start": 73445, "stop": 73648, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_009885728.1", "SO:0001217", "UniParc:UPI0000557CF6", "UniRef:UniRef100_UPI0000557CF6", "UniRef:UniRef50_UPI00027B32C6", "UniRef:UniRef90_UPI00027B32C6" ], "nt": "ATGGTAAAAAGAAAGCGAAAGCCTAAGCTTAATTCGCGTAATATTTTAACTATCCAGATTGTTTTAACAATCTTTAGTATGATCTTTTTTCTTACTTTGCTATCTTTAATCTTGTTTTTGAGTTTGCAAAGTAATTTAGCTACAGCTTTAGTTGAAAACAGAAATAAAGCTGTGGAACTTGTAGATAACATTGTCTTTTTTTAA", "aa": "MVKRKRKPKLNSRNILTIQIVLTIFSMIFFLTLLSLILFLSLQSNLATALVENRNKAVELVDNIVFF", "aa_hexdigest": "568c81a3bf058dd7575c3f7fb9d63054", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557CF6", "ncbi_nrp_id": "WP_009885728.1", "uniref100_id": "UniRef100_UPI0000557CF6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557CF6", "RefSeq:WP_009885728.1", "UniRef:UniRef100_UPI0000557CF6" ] }, "ips": { "uniref100_id": "UniRef100_UPI0000557CF6", "uniref90_id": "UniRef90_UPI00027B32C6" }, "psc": { "uniref90_id": "UniRef90_UPI00027B32C6", "uniref50_id": "UniRef50_UPI00027B32C6" }, "pscc": { "uniref50_id": "UniRef50_UPI00027B32C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B32C6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7751.332799999995, "isoelectric_point": 11.0533784866333 }, "id": "KKNAAMEMLJ_178", "locus": "GCA000027325_00139" }, { "type": "cds", "sequence": "contig_1", "start": 73690, "stop": 74580, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAAACCTAATTAAAAATGTTATTCGTTCTTTAAAGAGTGCTAAGATTGCTTTAATAGCGTTAACTTTTTTAATTTTTGTTGCTGTTGGTGGTTTTGTGTTGTTAAATAACACAGTTAATAATTTTAACGCTGCTTTTAACTATGTCACCCACACTGGTAAATTAAGCAATGCCATCATTAATGAGCGTTATGACTTTGGTAAATTAGAGTTTCAAGAACAGACCAATAATTCTCAGAATAGTAGCGACAGTTTTACTTTAACTTTAACTAATGATTCAAGAACAAGTTTTATTAATAATGCCTTGAGAACTAACCCTTCTTTGTATGAAGGATTAGTAACCCAAACTTTTAGCTATCAAAACAAAACTGAAATGACTGAAAAAACCAATATAGTTAATCAGTCTAAAATTATTGCTGCTAACAATCTTAACAATGCATTAAGTAAAGATAAACAGCTCTTAGTTTCAGGTCAACTTGAAAAACTAAATGCTGTTTTTCGGGAATATAAAGCTATTAATATTACTGACAAAAGTGTTTTTAAAAAATTGATAGTATCAGAACCTAATGATTTGGTAAATAGCCTAGTTATTTTTGATGGTCAAAATTTATCTAGCTCCAAACAAAGTGATTTCAATAATTTTTTAAATCAATTTAACGAAATTAAATCAAAGGGTAAAGATAATTTAAGTACTACTTTAAAAACTGGGCAGTATCAGGCATTTTTACAAACTCTTTTTGATTATGCTCAAGCGAGTGAAACAACATTAAAAGATCAGCTTCAAAAGTTAATTTCAAATCCAGATTCAAGTGAAACAAATCAGGTTAAAAATCTCTTTGATACTCCAAGTACGCTTACTAATATTGGGGGTCAATTAACCTTACAATGA", "aa": "MLNLIKNVIRSLKSAKIALIALTFLIFVAVGGFVLLNNTVNNFNAAFNYVTHTGKLSNAIINERYDFGKLEFQEQTNNSQNSSDSFTLTLTNDSRTSFINNALRTNPSLYEGLVTQTFSYQNKTEMTEKTNIVNQSKIIAANNLNNALSKDKQLLVSGQLEKLNAVFREYKAINITDKSVFKKLIVSEPNDLVNSLVIFDGQNLSSSKQSDFNNFLNQFNEIKSKGKDNLSTTLKTGQYQAFLQTLFDYAQASETTLKDQLQKLISNPDSSETNQVKNLFDTPSTLTNIGGQLTLQ", "aa_hexdigest": "b3dbeab7e324e33eb17cf30bebde3cb4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33060.81380000003, "isoelectric_point": 8.856682777404785 }, "id": "KKNAAMEMLJ_179", "locus": "GCA000027325_00140" }, { "type": "cds", "sequence": "contig_1", "start": 75085, "stop": 75393, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "TTGAATTTATTAGCAGCAAGAACAGTTTTTATCCCTAATCTAATTAACACAATTAATTTAGTGGCTTTGTTTTTAACTATTGCTATCCTAACTGTTGCTATAATTGTCAGCATTTTAATCCTGATTAGTTATTTAAAGAAAAACACTGAGCAAATTGGCATTTTAAAAGCAAATGGGTTAAGTGGTAAAAAGATTAACCTTAGCTTGTTAATCTTTGGGTTAATTCCTGCTATAGTAGGTGCTATTTCTGGATATAGCTTTGGAATTGGATTTCAAGACGTAGCTATTCATCTATTTAGTAACTATTGA", "aa": "MNLLAARTVFIPNLINTINLVALFLTIAILTVAIIVSILILISYLKKNTEQIGILKANGLSGKKINLSLLIFGLIPAIVGAISGYSFGIGFQDVAIHLFSNY", "aa_hexdigest": "7259c36bcdd016408920d3f67883d822", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 74, "aa_cov": 0.7156862745098039, "hmm_cov": 1.0, "evalue": 7.646808665903128e-11, "score": 37.230735778808594, "start": 24, "stop": 96 } ], "seq_stats": { "molecular_weight": 10943.102899999989, "isoelectric_point": 9.777226829528807 }, "id": "KKNAAMEMLJ_180", "locus": "GCA000027325_00141" }, { "type": "cds", "sequence": "contig_1", "start": 75436, "stop": 75642, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTTTCACTGTTTGTTATCTTAATTATGAGTAGTATATCGCTTTTAGTGGGATCAATTATCTTAAAGAAGGATGTTGTAAAGATTTTAAAGCATGACAGTGAATTTAAAGTTTCAAGATTAGGACTTAGTTCTAAGAAATTGTTTGCTAGGTTTGGTATTATGACCAGGTTTAGAGTAGCATTAGCATTTAACGCTCCTTGA", "aa": "MFFSLFVILIMSSISLLVGSIILKKDVVKILKHDSEFKVSRLGLSSKKLFARFGIMTRFRVALAFNAP", "aa_hexdigest": "8beeb4b01f9c33f95dd04916f08798f9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7677.318799999996, "isoelectric_point": 11.13389949798584 }, "id": "KKNAAMEMLJ_181", "locus": "GCA000027325_00142" }, { "type": "cds", "sequence": "contig_1", "start": 75664, "stop": 75816, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTCATTTACAATGATGATTTTAAACCTTAGTTTTGCAACTAAAGATAGCTTTGAAAATGCTCAATCAAAAACTAATTTAACTAATCAGAACCACCAATATGAATTTGAACTCGCTTCAGCAACAACACAAAGTGGTTTATTGAAGTGA", "aa": "MSSFTMMILNLSFATKDSFENAQSKTNLTNQNHQYEFELASATTQSGLLK", "aa_hexdigest": "7a78ee8852ef66890264e9313cd3a180", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5620.217699999999, "isoelectric_point": 5.510108375549315 }, "id": "KKNAAMEMLJ_182", "locus": "GCA000027325_00143" }, { "type": "cds", "sequence": "contig_1", "start": 76078, "stop": 76572, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACTAACCAAGCATCTGCTTCCAACACTGCTTTTCAAAATTTTTTAAAAGCAATCATTACTATAAATCCAAGCCAAGGATCGCAATTCATTAAACAAACCCAAGATCCATTAACAAAAAGATTTATCTATGCAATTGACAGTGATAAGGCATTAAAAAATAATAATGAACAAAACGGTTCCCAAAACCACTTAACTTTAAATGATGATTTTGCTAAATTTCTCTACAGTCAATTTGAATTAATTAAAAAGAGTGGGAATGCAAGTAATGAAGATTTAAATGCAATTGATTTTGAAAACCCCCAAACAATCAGAGATTTTTACAACAAGTACAATGCTTTACCACCATTAGATTACAAACTTAGCTTTAATGTAATAGGTTTACCCAAAGAGACAATTGCTGGACAAATTGACACCCCTAAGTATGGATTTTTAACCCTTCATGGTGAATATCAAAATACTCCTATCAAGATTAAAGGTATTAAAGATTGA", "aa": "MPTNQASASNTAFQNFLKAIITINPSQGSQFIKQTQDPLTKRFIYAIDSDKALKNNNEQNGSQNHLTLNDDFAKFLYSQFELIKKSGNASNEDLNAIDFENPQTIRDFYNKYNALPPLDYKLSFNVIGLPKETIAGQIDTPKYGFLTLHGEYQNTPIKIKGIKD", "aa_hexdigest": "d6c71fb82a4e129340b4ab333a220b62", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18527.53399999999, "isoelectric_point": 6.73123722076416 }, "id": "KKNAAMEMLJ_183", "locus": "GCA000027325_00144" }, { "type": "cds", "sequence": "contig_1", "start": 76582, "stop": 77637, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F" ], "nt": "GTGGATAATTTAGGTCCAGTTTTGAGTGATCAAAACAACCACATTATTAATCAAGAATTGTTTAAAAATTATTCTTTTGATCCTTTGATAGTTAACAATTCTGCTGCAAAAAAATACCAACTTGCAATAGGTAGTGAGATTAATATTGCAGTTAACAACAGCTTCAAACGGATTGACAATAAGATCATTAATCAAGATCCTTTAGTGAATGCTACCTTTAGAGTTGTAGGGATTAACAATTCCGCTCATGATCCTGAATTTTTCACTAGTTATAGTACTGCTTTTAAAGTATTGGAATATCCCAATGAATGGTTCGTAAAAAAACTTCCATTTAATAGCTTCTATGCTAATTCGCTTTTAAGTTTTGTTCAATCTACTTCGCTATTTTCTGAATCTGGTATTTTTCCTGCTACTAGTAGTTTTTCAACTAATAACACTGTACTTGTTGAGTTAATTAAAAAAACCATTAATTACAAGAATGGTCAAATGAATCAAACTTCAAGTAATGACTCTTCTAAGAAAGAAAATTACCAAAAATTGCAAAAAGCATTAGGAATATCAACTGATTTGGAGATTAGTAAAGTTAATGAATATGTTGCTATCTTAGCAAGGGTTTATAATGGTTTACCTTACAACTCTACTATTAGCTTTATTAGCAATGTTGCTGCTAACAACGCTTTATTTGGAAATATTGCTAACACCACCAAGCAGATTCAAGCTGTTGTAATTGCAGTGATTATTCCTATAATCATGTTGATTATTCTTTTGGTTTCAACTACCTTAATTCAAGAGTTGAAAAAAATTGCTATTAGATTAAAAGCATTGGGATATTCCAATTTAAAAATTCTCGCTTCATTTTTATCAATATACATCCCTTTATTTGCCTTTGGTTTGTTGATTTCTATCCCCTTTTCTATCTATCTAATTGCACTACATAATGAGGTAATTTTTGCAAGCTCATCGATCTTTTTAGATGCTTTTTTAAGTTTTGAAAGTGCAATTGGTTCAATGTTAGTTTTACTAGCGGTTTTATCAATTACCTTTGTGTTGAATTGA", "aa": "MDNLGPVLSDQNNHIINQELFKNYSFDPLIVNNSAAKKYQLAIGSEINIAVNNSFKRIDNKIINQDPLVNATFRVVGINNSAHDPEFFTSYSTAFKVLEYPNEWFVKKLPFNSFYANSLLSFVQSTSLFSESGIFPATSSFSTNNTVLVELIKKTINYKNGQMNQTSSNDSSKKENYQKLQKALGISTDLEISKVNEYVAILARVYNGLPYNSTISFISNVAANNALFGNIANTTKQIQAVVIAVIIPIIMLIILLVSTTLIQELKKIAIRLKALGYSNLKILASFLSIYIPLFAFGLLISIPFSIYLIALHNEVIFASSSIFLDAFLSFESAIGSMLVLLAVLSITFVLN", "aa_hexdigest": "f9180b4f61982dc951b184d8cb4df88e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0011815B2F", "query_cov": 1.0427350427350428, "subject_cov": 0.8413793103448276, "identity": 0.617, "score": 389.0, "evalue": 1.61e-130, "valid": false, "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI0011815B2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0011815B2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F" ], "product": "ABC transporter permease" }, "genes": [], "id": "KKNAAMEMLJ_184", "locus": "GCA000027325_00145" }, { "type": "cds", "sequence": "contig_1", "start": 77685, "stop": 77885, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTTTCTCTTTAAACAAAATCATAAAACCCAACCAGAAATTCACTAGTAATGAAGCTGAATTTCTACAGATAGCTACTGATTATTTGGAGGAAAGTCAAAACTATCTTCAAAAGGGTTTAAAGCAATTAAAAAAAGAATATAAAAGATCCATTATTTATAACCCTAACCTTGAATATAAACGCTTTGTTAAATGA", "aa": "MDFFSLNKIIKPNQKFTSNEAEFLQIATDYLEESQNYLQKGLKQLKKEYKRSIIYNPNLEYKRFVK", "aa_hexdigest": "98c702ed2232c26b335f4205013eaed0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8026.137799999998, "isoelectric_point": 9.397765922546387 }, "id": "KKNAAMEMLJ_185", "locus": "GCA000027325_00146" }, { "type": "cds", "sequence": "contig_1", "start": 78396, "stop": 79079, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9E0" ], "nt": "GTGTATAAATACATCACTAATGGCATTACTACAAATGCAGTTCTTAAAGGAGTTGATCTTGCCATTAAAAGTCATGATTTTATTGTGATTTTAGGCCCTTCAGGATCTGGTAAAACCACATTACTAAACATTATTTCAGGGATGGATAGAGCTTCTAGTGGTAGTGTTATTGTCAATGGTTATAACATGATTTGTTTAAATGATAGAAAGCTCACTAAATTCCGTCAAAAGTATGTTGGTTACATCTTTCAACAATACGGTTTATTACCTAATTTAACAGTTAGAGAAAACATTGAGATAGGAGCAAATCTTCAACCAGATCCTAGTAAAAGGATCAGCATTGATGCACTTTTAGAAGCGGTTGGGATGGATAGTTTGCAAAAGAAGCTTCCTAATGAATTGAGTGGTGGGCAACAGCAACGTGTTTCCATTGCAAGAGCTTTTGCTAAAAACCCCTTATTAATTTTTGGTGATGAACCTACTGGGGCACTTGATCTTGAGATGACCCAAATTGTTTTAAAACAGTTTTTAGCAATTAAAAAGCGTTATCAAACGACAATGATTATTGTTACCCACAACAATTTAATTGCTAACTTAGCTGATTTAGTTATCTATGTAGCAGATGGAAAAATAAAATCACTACACAGGAACTTAAATCCTAAACAGGTTGAAGAGATCCATTGA", "aa": "MYKYITNGITTNAVLKGVDLAIKSHDFIVILGPSGSGKTTLLNIISGMDRASSGSVIVNGYNMICLNDRKLTKFRQKYVGYIFQQYGLLPNLTVRENIEIGANLQPDPSKRISIDALLEAVGMDSLQKKLPNELSGGQQQRVSIARAFAKNPLLIFGDEPTGALDLEMTQIVLKQFLAIKKRYQTTMIIVTHNNLIANLADLVIYVADGKIKSLHRNLNPKQVEEIH", "aa_hexdigest": "bc7686ff677e6c1249c40cc7f03caf28", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P9E0", "query_cov": 0.8766519823788547, "subject_cov": 0.9900497512437811, "identity": 0.698, "score": 267.0, "evalue": 3.76e-88, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3B0P9E0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P9E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9E0" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "KKNAAMEMLJ_186", "locus": "GCA000027325_00147" }, { "type": "cds", "sequence": "contig_1", "start": 79089, "stop": 79940, "strand": "+", "frame": 3, "gene": "tkt", "product": "transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "EC:2.2.1.1", "GO:0004802", "GO:0005829", "GO:0006098", "GO:0046872", "KEGG:K00615", "SO:0001217", "UniRef:UniRef50_P75611", "UniRef:UniRef90_P47312" ], "nt": "ATGAAATACTTATATGCCACTCAACACCTTACTTTAAATGCTATTAAGCATGCTAAGGGAGGACATGTTGGCATGGCCATTGGTGCAAGTCCTATCTTATTTAGTTTATTTACTAAACACTTTCACTTTGATCCTGACCAACCAAAGTGGATCAACAGAGATCGCTTTGTTTTAAGTGCTGGCCATGGTAGCATGGCATTATATTCAATTTTCCATTTTGCCGGACTTATTTCTAAACAAGAGATCTTACAGCATAAACATGGTCAAATTAACACTTCTTCCCATCCTGAATATGCTCCAAATAACTTCATAGATGCATCAACAGGCCCTTTAGGTCAAGGCTTTGGCATGGCAGTTGGCATGGTGTTAGCACAAAAGTTATTAGCTAATGAATTTAAAGAGCTAAGTGATAAATTGTTTGACCATTACACCTATGTGGTTGTTGGGGATGGAGATCTACAGGAGGGGGTTAGTTATGAAGTTAGTCAAATTGCTGGGTTATATAAATTAAATAAACTAATTGTGCTTCATGATTCAAATAGAGTGCAAATGGATAGTGAAGTAAAAAAAGTTGCTAATGAAAATCTAAAGGTTAGGTTTGAAAACGTTGGTTGGAATTACATCCATACTGATGATCAACTAGAAAATATTGATCAAGCTATTATTAAAGCCAAACAATCAGATAAGCCAACTTTTATTGAAGTGAGAACAACTATTGCTAAAAACACCCACCTTGAAGATCAGTATGGAGGACATTGGTTTATTCCCAATGAAGTGGACTTTCAACTTTTTGAGAAAAGAACAAATACTAACTTTAACTTTTTTAATTATCCAGATAGTATTTACCACTGA", "aa": "MKYLYATQHLTLNAIKHAKGGHVGMAIGASPILFSLFTKHFHFDPDQPKWINRDRFVLSAGHGSMALYSIFHFAGLISKQEILQHKHGQINTSSHPEYAPNNFIDASTGPLGQGFGMAVGMVLAQKLLANEFKELSDKLFDHYTYVVVGDGDLQEGVSYEVSQIAGLYKLNKLIVLHDSNRVQMDSEVKKVANENLKVRFENVGWNYIHTDDQLENIDQAIIKAKQSDKPTFIEVRTTIAKNTHLEDQYGGHWFIPNEVDFQLFEKRTNTNFNFFNYPDSIYH", "aa_hexdigest": "73163c6fd2734abb2256931d156284e5", "start_type": "ATG", "rbs_motif": null, "genes": [ "tkt" ], "truncated": "3-prime", "pseudogene": { "start": 79089, "stop": 80243, "observations": { "mutation": [ 79938 ], "stop": [ 79938 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47312", "query_cov": 1.0, "subject_cov": 0.4367283950617284, "identity": 1.0, "score": 581.0, "evalue": 2.01e-204, "gene_start": 1, "gene_end": 283, "reference_start": 1, "reference_end": 283, "reference_sequence": "MKYLYATQHLTLNAIKHAKGGHVGMAIGASPILFSLFTKHFHFDPDQPKWINRDRFVLSAGHGSMALYSIFHFAGLISKQEILQHKHGQINTSSHPEYAPNNFIDASTGPLGQGFGMAVGMVLAQKLLANEFKELSDKLFDHYTYVVVGDGDLQEGVSYEVSQIAGLYKLNKLIVLHDSNRVQMDSEVKKVANENLKVRFENVGWNYIHTDDQLENIDQAIIKAKQSDKPTFIEVRTTIAKNTHLEDQYGGHWFIPNEVDFQLFEKRTNTNFNFFNYPDSIYHWFKQTVIERQKQIKEDYNNLLISLKDKPLFKKFTNWIDSDFQALYLNQLDEKKVAKKDSATRNYLKDFLNQINNPNSNLYCLNADVSRSCFIKIGDDNLHENPCSRNIQIGIREFAMATIMNGMALHGGIKVMGGTFLAFADYSKPAIRLGALMNLPVFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVCVFRPCDEKETCAGFNYGLLSQDQTTVLVLTRQPLKSIDNTDSLKTLKGGYILLDRKQPDLIIAASGSEVQLAIEFEKVLTKQNVKVRILSVPNITLLLKQDEKYLKSLFDANSSLITIEASSSYEWFCFKKYVKNHAHLGAFSFGESDDGDKVYQQKGFNLERLMKIFTSLRN" }, "paralog": false, "identity": 0.9973958333333334, "score": 785.0, "evalue": 6.44e-287, "description": "Internal stop codon at 79938. Nonsense mutation around 79938.", "psc": { "uniref90_id": "UniRef90_P47312", "gene": "tkt", "product": "transketolase", "ec_ids": [ "2.2.1.1" ], "uniref50_id": "UniRef50_P75611", "cog_id": "COG0021", "cog_category": "G", "kegg_orthology_id": "K00615", "go_ids": [ "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47312", "EC:2.2.1.1", "UniRef:UniRef50_P75611", "COG:COG0021", "COG:G", "KEGG:K00615", "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ] }, "pscc": { "uniref50_id": "UniRef50_P75611", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75611" ], "product": "Transketolase" } }, "id": "KKNAAMEMLJ_187", "locus": "GCA000027325_00148" }, { "type": "cds", "sequence": "contig_1", "start": 79995, "stop": 81035, "strand": "+", "frame": 3, "gene": "tkt", "product": "transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "EC:2.2.1.1", "GO:0004802", "GO:0005829", "GO:0006098", "GO:0046872", "KEGG:K00615", "SO:0001217", "UniRef:UniRef50_P75611", "UniRef:UniRef90_P47312" ], "nt": "TTGCTAATTTCTCTTAAAGACAAACCACTTTTTAAAAAATTTACTAATTGGATTGACAGTGATTTTCAAGCCCTTTATCTTAACCAACTAGATGAAAAGAAAGTAGCAAAAAAAGATAGTGCTACTAGAAACTATTTAAAAGATTTTTTAAACCAAATTAATAATCCTAATTCCAACTTGTATTGCTTAAATGCTGATGTATCACGTTCTTGTTTTATCAAGATAGGTGATGATAATCTCCATGAAAATCCTTGTTCTAGAAATATCCAAATAGGAATTAGGGAGTTTGCAATGGCAACAATAATGAATGGTATGGCACTTCATGGTGGTATTAAAGTGATGGGTGGTACTTTTTTAGCATTTGCTGATTATTCAAAGCCAGCAATTCGCTTAGGTGCATTAATGAACTTACCAGTATTTTATGTTTATACCCATGACTCTTATCAAGTAGGGGGTGATGGTCCTACTCATCAACCCTATGATCAACTACCAATGTTAAGAGCAATTGAAAATGTTTGTGTATTTCGTCCTTGTGATGAAAAGGAAACTTGTGCTGGATTTAACTATGGTCTTTTAAGTCAAGATCAGACAACTGTTTTGGTTTTAACACGTCAACCCTTAAAATCCATTGATAACACTGATAGTTTAAAAACACTGAAGGGTGGTTATATCCTTTTGGATAGAAAACAACCTGATTTAATTATTGCTGCTAGTGGTAGTGAAGTGCAACTTGCAATAGAGTTTGAAAAAGTTTTAACTAAACAAAATGTAAAGGTAAGAATTCTGTCAGTTCCCAATATAACTTTACTTTTAAAACAAGATGAAAAATATCTAAAGAGTTTATTTGATGCTAACAGTTCACTTATCACCATAGAAGCTAGTAGTAGCTATGAGTGGTTTTGCTTTAAGAAGTATGTTAAAAACCATGCTCATTTAGGAGCTTTTAGTTTTGGTGAATCTGATGATGGAGATAAAGTTTATCAGCAAAAAGGGTTTAATCTGGAAAGGTTAATGAAAATATTTACTTCCCTAAGAAATTAA", "aa": "MLISLKDKPLFKKFTNWIDSDFQALYLNQLDEKKVAKKDSATRNYLKDFLNQINNPNSNLYCLNADVSRSCFIKIGDDNLHENPCSRNIQIGIREFAMATIMNGMALHGGIKVMGGTFLAFADYSKPAIRLGALMNLPVFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVCVFRPCDEKETCAGFNYGLLSQDQTTVLVLTRQPLKSIDNTDSLKTLKGGYILLDRKQPDLIIAASGSEVQLAIEFEKVLTKQNVKVRILSVPNITLLLKQDEKYLKSLFDANSSLITIEASSSYEWFCFKKYVKNHAHLGAFSFGESDDGDKVYQQKGFNLERLMKIFTSLRN", "aa_hexdigest": "31b30f354eadb7e09511a3ca7f175f69", "start_type": "TTG", "rbs_motif": null, "genes": [ "tkt" ], "truncated": "5-prime", "pseudogene": { "start": 79695, "stop": 81035, "observations": { "mutation": [ 79938 ], "stop": [ 79938 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47312", "query_cov": 1.0, "subject_cov": 0.5339506172839507, "identity": 0.997, "score": 699.0, "evalue": 1.22e-249, "gene_start": 1, "gene_end": 346, "reference_start": 303, "reference_end": 648, "reference_sequence": "MKYLYATQHLTLNAIKHAKGGHVGMAIGASPILFSLFTKHFHFDPDQPKWINRDRFVLSAGHGSMALYSIFHFAGLISKQEILQHKHGQINTSSHPEYAPNNFIDASTGPLGQGFGMAVGMVLAQKLLANEFKELSDKLFDHYTYVVVGDGDLQEGVSYEVSQIAGLYKLNKLIVLHDSNRVQMDSEVKKVANENLKVRFENVGWNYIHTDDQLENIDQAIIKAKQSDKPTFIEVRTTIAKNTHLEDQYGGHWFIPNEVDFQLFEKRTNTNFNFFNYPDSIYHWFKQTVIERQKQIKEDYNNLLISLKDKPLFKKFTNWIDSDFQALYLNQLDEKKVAKKDSATRNYLKDFLNQINNPNSNLYCLNADVSRSCFIKIGDDNLHENPCSRNIQIGIREFAMATIMNGMALHGGIKVMGGTFLAFADYSKPAIRLGALMNLPVFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVCVFRPCDEKETCAGFNYGLLSQDQTTVLVLTRQPLKSIDNTDSLKTLKGGYILLDRKQPDLIIAASGSEVQLAIEFEKVLTKQNVKVRILSVPNITLLLKQDEKYLKSLFDANSSLITIEASSSYEWFCFKKYVKNHAHLGAFSFGESDDGDKVYQQKGFNLERLMKIFTSLRN" }, "paralog": false, "identity": 0.9977578475336323, "score": 905.0, "evalue": 0.0, "description": "Internal stop codon at 79938. Nonsense mutation around 79938.", "psc": { "uniref90_id": "UniRef90_P47312", "gene": "tkt", "product": "transketolase", "ec_ids": [ "2.2.1.1" ], "uniref50_id": "UniRef50_P75611", "cog_id": "COG0021", "cog_category": "G", "kegg_orthology_id": "K00615", "go_ids": [ "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47312", "EC:2.2.1.1", "UniRef:UniRef50_P75611", "COG:COG0021", "COG:G", "KEGG:K00615", "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ] }, "pscc": { "uniref50_id": "UniRef50_P75611", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75611" ], "product": "Transketolase" } }, "id": "KKNAAMEMLJ_188", "locus": "GCA000027325_00149" }, { "type": "cds", "sequence": "contig_1", "start": 81085, "stop": 81294, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGCCTGGTTGCATCTTACTTTCATCCTGTTCTTTTAGAAGTTATATCCCAACTCCTAGTTTAAGAAATACTGTTGGTAATCACAACAGTTATGTTAATAATACTGTCCCTAAAAACAATTTTTATGAAAAGTTTTATGATCTAACTTTTGCTTTAAATTTCACTAATCAGAAAACTCAAGAGTTTGGTACTGGTTGGTTAATTGACTGA", "aa": "MPGCILLSSCSFRSYIPTPSLRNTVGNHNSYVNNTVPKNNFYEKFYDLTFALNFTNQKTQEFGTGWLID", "aa_hexdigest": "fc002f78baf29bb0d383dafd8541c01e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 39, "aa_cov": 0.4492753623188406, "hmm_cov": 1.0, "evalue": 4.7825653466608957e-08, "score": 28.07671546936035, "start": 39, "stop": 69 } ], "seq_stats": { "molecular_weight": 7916.801200000002, "isoelectric_point": 7.786185264587402 }, "id": "KKNAAMEMLJ_189", "locus": "GCA000027325_00150" }, { "type": "cds", "sequence": "contig_1", "start": 81529, "stop": 81714, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTAGGTTTGCCAATCCTAAGATAAATTCCAAACCAGAAGAGATGAACTACCAAGTTCAAACTCGTTTAAAACAAGATGCAATGGTGTTTATCCAAACCAGTACTTTACCTAAAACTGCTTATGCAGGAATTGATCCTATTAACTTTGATTACCATGAAACTAGTGATGAGAGTGGATTTTGA", "aa": "MGRFANPKINSKPEEMNYQVQTRLKQDAMVFIQTSTLPKTAYAGIDPINFDYHETSDESGF", "aa_hexdigest": "ae25d8c8c9459c672b3e39e998832611", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6972.7134, "isoelectric_point": 5.082394981384277 }, "id": "KKNAAMEMLJ_190", "locus": "GCA000027325_00151" }, { "type": "cds", "sequence": "contig_1", "start": 82620, "stop": 84044, "strand": "+", "frame": 3, "gene": null, "product": "putative lipoprotein MG068", "db_xrefs": [ "GO:0005886", "RefSeq:WP_009885731.1", "SO:0001217", "UniParc:UPI00001391E8", "UniRef:UniRef100_P47314", "UniRef:UniRef50_P47314", "UniRef:UniRef90_P47314" ], "nt": "GTGTTAAGACGTTATCTAACTCTGAGTTTTTCTAGCTTGCTTTTATTAGCACTGCTGTTTTTAACAGGCTGTTCTTTTGTTAGACCACAATTTCGCAGGGGCTTTAGAACCCAGTTTAAAATTAACTCTATTCCAACTGTTAGTGATCCTTATCACATTAACTACGACTTAACTTTTTCATTAAACTTTGCTTCCAACAAAAGAAATACTTATGGTACTGGTTGGTTAATTGATTGGAAAGGAGATGAAAATAACCCTGAGAAAAATGATCCTTTTAAAGTTTATTTAGCTACTAACCTCCATGTGATAGATGCTTTAAGAAATAATAATGACTATGAACCATATAACAAGGATAGTAATAACCAAGCTTTTAATAGTGAAGAGATCACTAGGTTCTTTTCTATAGGTAAATACACATATCCTAGTATTTTTAGTGAATTAAATTTCATTATCAATGCTAGAGAAGCGTTTGTTTCGATTCAAACCTCTACTATTCCTAAAACTGCTTATGCTGCTGTTAACTTTGTTGAAACACAAGGAGAGGATGAAAGTTATACAGATTCATTATCAACTGATAATAAAAGAGATATTTATGCGGATTTTGCTGTGATAGAAATTCCCTTATTCTTAACTAACCATCGCGATTATCAAGTATTTAATGAATTTATTAAACCAGCAATAGAAACATACAAACAACTAGGAAACTCTTCCTTTGAAAAAAAACAACTAGATCAACATAAAAACGACAACTTTTACATGTTAGGTTATCCTTTGGTTGAGAGTAGCATAGATGCTCTAATTTTGAACCAAAGAAGACAGTACAATAACAGTTATACTGAAAAATATACTCCTCAAACTTTAACCAAAGATCAACGTACCATTGACTTAAGCAGAGAAGTTCCTACTTTAATTCAGAACAAAACAGAAAACTCTACAGGAAGTCAATTACTAGTAAATCAATCTTTAAGTAGTACATCTGAAGGGATTATTGAATTTATTAAGTTACCTGAATTTAAACTCAATTATCATAATAAAAGTTACCGTCAATATGGTAGAGGTTTTGCTCTACAAAACACTAACTTTAGACCAGGTTCTTCAGGAACTTTAATGTTAAATAACCAAAAACAGATAGCAGGTATTTATTTTGGTGTTTTAGATTTTGGAGAAGATGTAAGTTTAATGAGTAACATTGGCGTTGGACAAATTCTTCGTGTTCCTCAAAAGAACAATACTAGAAATAGATCAATTGCTACTAATAAAAGCAACTATGATTTGATCTTTGGTGATAGCAATACAACTAACTTTTATGCAAAATTTGCTAGACAAAATAACACCCATCTTTATCAGATGATTTCAAATAGTAAAGATACAAAATTAAAGTATGTGAATACTGTTGAAAAAACAGTAAAAGCTAGTATTAAATAA", "aa": "MLRRYLTLSFSSLLLLALLFLTGCSFVRPQFRRGFRTQFKINSIPTVSDPYHINYDLTFSLNFASNKRNTYGTGWLIDWKGDENNPEKNDPFKVYLATNLHVIDALRNNNDYEPYNKDSNNQAFNSEEITRFFSIGKYTYPSIFSELNFIINAREAFVSIQTSTIPKTAYAAVNFVETQGEDESYTDSLSTDNKRDIYADFAVIEIPLFLTNHRDYQVFNEFIKPAIETYKQLGNSSFEKKQLDQHKNDNFYMLGYPLVESSIDALILNQRRQYNNSYTEKYTPQTLTKDQRTIDLSREVPTLIQNKTENSTGSQLLVNQSLSSTSEGIIEFIKLPEFKLNYHNKSYRQYGRGFALQNTNFRPGSSGTLMLNNQKQIAGIYFGVLDFGEDVSLMSNIGVGQILRVPQKNNTRNRSIATNKSNYDLIFGDSNTTNFYAKFARQNNTHLYQMISNSKDTKLKYVNTVEKTVKASIK", "aa_hexdigest": "26e245a780834f6b3b7e7bd2dd38444c", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001391E8", "ncbi_nrp_id": "WP_009885731.1", "uniref100_id": "UniRef100_P47314", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391E8", "RefSeq:WP_009885731.1", "UniRef:UniRef100_P47314" ] }, "ips": { "uniref100_id": "UniRef100_P47314", "uniref90_id": "UniRef90_P47314" }, "psc": { "uniref90_id": "UniRef90_P47314", "product": "Uncharacterized lipoprotein MG068", "uniref50_id": "UniRef50_P47314", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47314", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47314" ], "product": "Uncharacterized lipoprotein MG068" }, "genes": [], "id": "KKNAAMEMLJ_191", "locus": "GCA000027325_00152" }, { "type": "cds", "sequence": "contig_1", "start": 84082, "stop": 84312, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3523", "UniRef:UniRef90_UPI00027B3523" ], "nt": "ATGGAAAATCAAAACAGTTGTAGTTCTGTAAAAGAAAAAGAGTTATATAATCCAAAAAAGCCTTTTCTTTTTGCGGTAATTTCTTTCTTTGTTTTCATAATATTTTTTGCTTTAGCAGGTTCATTTACCAAATTTGCATTAGAAGAAAGCAGGGGTTATAGTTTTCTTGTTATTGTCTGTTATCTTCTAGGAACAGTTTGTTTTTTTGTTTTTATCTATTTTTGGTTTTGA", "aa": "MENQNSCSSVKEKELYNPKKPFLFAVISFFVFIIFFALAGSFTKFALEESRGYSFLVIVCYLLGTVCFFVFIYFWF", "aa_hexdigest": "d5f8cc2f119890b53d673f693385b9d3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 84082, "stop": 84408, "observations": { "mutation": [ 84310, 84355 ], "stop": [ 84310, 84355 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00027B3523", "query_cov": 0.9342105263157895, "subject_cov": 0.6574074074074074, "identity": 0.986, "score": 135.0, "evalue": 1.1e-39, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MENQNSCSSVKEKELYNPKKSFLFAVISFFVFIIFFALAGSFTKFALEESRGYSFLVIVCYLLGTVCFFVFIYFWFWFLSEFFANRVLVKLWDLPTKKRRKRHAKKQR" }, "paralog": false, "identity": 0.9351851851851852, "score": 198.0, "evalue": 2.62e-67, "description": "Internal stop codon at 84310, 84355. Nonsense mutation around 84310, 84355.", "psc": { "uniref90_id": "UniRef90_UPI00027B3523", "uniref50_id": "UniRef50_UPI00027B3523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00027B3523", "UniRef:UniRef50_UPI00027B3523" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3523" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8931.456899999994, "isoelectric_point": 7.674423408508302 }, "id": "KKNAAMEMLJ_192", "locus": "GCA000027325_00153" }, { "type": "cds", "sequence": "contig_1", "start": 84532, "stop": 84744, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTATTTGGTAATCCTTGTTTAGTAGATCCTAATAAAATGAAATTAAATGCTAATACACTAACAACAACTAAGAGTGTTATTAATATTGATCACAACAAAATTAAAGTTGTTGTTAGAAATTTAACTTTGCTTGTTTCTATTTCATTTAAACAAGTCTTTTTATCATTTGAAATATTTAATTCTTTTTGCACTTTCCAACTAATAAAATAA", "aa": "MVFGNPCLVDPNKMKLNANTLTTTKSVINIDHNKIKVVVRNLTLLVSISFKQVFLSFEIFNSFCTFQLIK", "aa_hexdigest": "5323dbaff72f7840aa10d85d3e23a558", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7977.432699999995, "isoelectric_point": 9.698317527770996 }, "id": "KKNAAMEMLJ_193", "locus": "GCA000027325_00154" }, { "type": "cds", "sequence": "contig_1", "start": 84776, "stop": 85051, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTATCACAAAATGGTGTGATCTTATCAATAGCACTACTTGCTTTTTTTAGTTGGATGGCAATTGTTGGAATTCACAGCTTTTTAGTTGGAATTTTAGTTAATCATCAAACACTTAAAATAAGTAAAAAGTATGTTATTTTAGGTTCGATTTTTCCAATTATGGCATTAACAAATACTCTTGTAATTAGAAAAAAATTAAAAGCTTTATTAGGAGAGGGTAAGGTTCAAAAAGGACTCAAAGTTTTAACTATTAGTTTTATTTGTGTTTGA", "aa": "MQLSQNGVILSIALLAFFSWMAIVGIHSFLVGILVNHQTLKISKKYVILGSIFPIMALTNTLVIRKKLKALLGEGKVQKGLKVLTISFICV", "aa_hexdigest": "4dcb39ac6a88b6efc42fbcfc927a4b2b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9981.20659999999, "isoelectric_point": 10.37175426483154 }, "id": "KKNAAMEMLJ_194", "locus": "GCA000027325_00155" }, { "type": "cds", "sequence": "contig_1", "start": 85319, "stop": 85555, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED97BE8", "UniRef:UniRef90_UPI001ED97BE8" ], "nt": "TTGGCTTTAACCTTAATTGATTTAATGGGTTTAATTTTGGCTTTTGGTATTATTGTTGTTAACAATACAAATTCTTCAAGTGTTGATGCAAAATCAAATTCCTTAGCATTAATTTTTAAGTTCATTTATTTTGGTAGTTCTATAGCAGCCTTTTTAACAGTTTTAAGTTTTGCTAGTTCAGTTGCTTTACTTGTCAATTTAATAAAAAAAGATAAGGGATTAACCTTAAAAAATTAG", "aa": "MALTLIDLMGLILAFGIIVVNNTNSSSVDAKSNSLALIFKFIYFGSSIAAFLTVLSFASSVALLVNLIKKDKGLTLKN", "aa_hexdigest": "6a1291caebbbe8f881efecf830128b75", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 85055, "stop": 85555, "observations": { "mutation": [ 85082, 85226, 85292 ], "stop": [ 85082, 85226, 85292 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001ED97BE8", "query_cov": 1.0, "subject_cov": 0.46987951807228917, "identity": 0.987, "score": 134.0, "evalue": 1.38e-38, "gene_start": 1, "gene_end": 78, "reference_start": 89, "reference_end": 166, "reference_sequence": "MQLIIGFFVWLFPYAGEAGINIGINLSIFNLAQLVGSDINVITYFTTFLTLLFAFLSWAVLHVLACFILVHLTIYKQQEWIKIKAIYILALTLIDLMGLILAFGIIVVNNTNSSSVDAKSNSLALIFKFIYFGSSIAAFLTVLSFASSVALLVNLIKKDKGLTLKN" }, "paralog": false, "identity": 0.9759036144578314, "score": 301.0, "evalue": 7.97e-107, "description": "Internal stop codon at 85082, 85226, 85292. Nonsense mutation around 85082, 85226, 85292.", "psc": { "uniref90_id": "UniRef90_UPI001ED97BE8", "uniref50_id": "UniRef50_UPI001ED97BE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001ED97BE8", "UniRef:UniRef50_UPI001ED97BE8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001ED97BE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED97BE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8281.80509999999, "isoelectric_point": 9.602839851379393 }, "id": "KKNAAMEMLJ_195", "locus": "GCA000027325_00156" }, { "type": "cds", "sequence": "contig_1", "start": 85589, "stop": 85942, "strand": "+", "frame": 2, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDG5", "UniRef:UniRef90_UPI000303F145" ], "nt": "GTGCCTAAGGTGGTATTGGAGTATCACAAGCTCAGTAAGGATGTAGTCAAAGAGAGTTTGGGGGTGGATACATCAGGTTCAACTTTTGATCCAACTAAAAGGTTGAAAAAAGATAGTCCAATGAAGGATTCAAACAAAGACAGTGAGAAACTCACCGAAGCAACTGCTTCATCCATGAGTAGTGGTGGGGCTACATCCACTCGCAAGGCCCTCAAGGTTGAGGTGGAGAAACAAAGTGGATCAACTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCACTAAAGCATAAAGAAAATAGTGGGGAGGTGAAGTTGGATGCGAGTGGGGAGTTTGCCAATGATAAAGCCTGA", "aa": "MPKVVLEYHKLSKDVVKESLGVDTSGSTFDPTKRLKKDSPMKDSNKDSEKLTEATASSMSSGGATSTRKALKVEVEKQSGSTDSLLKNDFAKKPLKHKENSGEVKLDASGEFANDKA", "aa_hexdigest": "2da47419c5fb6ff0636218b74a587264", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000303F145", "query_cov": 1.0, "subject_cov": 0.8602941176470589, "identity": 1.0, "score": 212.0, "evalue": 4.72e-69, "valid": true, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "genes": [], "id": "KKNAAMEMLJ_196", "locus": "GCA000027325_00157" }, { "type": "cds", "sequence": "contig_1", "start": 86025, "stop": 86141, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGTGGGGTAGAAGAGGAAGTAGAAGTAGTAGAAGAAGAAGAAGAAGGGGAGGGGGAGTTGGAGAAGGAATAA", "aa": "MIIDLTPTLMLEKVGSGVEEEVEVVEEEEEGEGELEKE", "aa_hexdigest": "72a0abf369d493f918669942fc75fd7e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4248.641399999999, "isoelectric_point": 4.0500284194946286 }, "id": "KKNAAMEMLJ_197", "locus": "GCA000027325_00158" }, { "type": "cds", "sequence": "contig_1", "start": 86263, "stop": 86535, "strand": "+", "frame": 1, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000311AB1E", "UniRef:UniRef90_UPI000311AB1E" ], "nt": "GTGAAACCTAGCAATAATAGTCAACAGTTTAATCCCAATTCAGATGATAATAAAGTCACTTCAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCGTTGACTTTCACTAATAAGAATAACCCGCAAAGAAACCAACTGTTGCTCAGAGCACTGTTAGGAACGATCCCGGTATTGATCAATAAGAGTGGAGGAAGTGGGAATGAGTTTACCCATACGAGTGAGCAGCAGTGA", "aa": "MKPSNNSQQFNPNSDDNKVTSGGSSKPTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGGSGNEFTHTSEQQ", "aa_hexdigest": "f2b0feb502379e791344cc4c286e80ef", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 86134, "stop": 86616, "observations": { "mutation": [ 86203, 86533 ], "stop": [ 86203, 86533 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000311AB1E", "query_cov": 1.0, "subject_cov": 0.5625, "identity": 0.9890000000000001, "score": 176.0, "evalue": 3.51e-55, "gene_start": 1, "gene_end": 90, "reference_start": 44, "reference_end": 133, "reference_sequence": "MITQHLNQQNTRWVFTPNSSPDIWTGAGYRKGSSNTNGIPFDQVKPSNNSQQFNPNSDDNKVTSGGSSKPTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGGSGNEFTHTSEQQWNETDKLGGNLPGFGEVNGGFYPVLLI" }, "paralog": false, "identity": 0.9875, "score": 327.0, "evalue": 6.49e-117, "description": "Internal stop codon at 86203, 86533. Nonsense mutation around 86203, 86533.", "psc": { "uniref90_id": "UniRef90_UPI000311AB1E", "product": "adhesin", "uniref50_id": "UniRef50_UPI000311AB1E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000311AB1E", "UniRef:UniRef50_UPI000311AB1E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000311AB1E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000311AB1E" ], "product": "adhesin" } }, "id": "KKNAAMEMLJ_198", "locus": "GCA000027325_00159" }, { "type": "cds", "sequence": "contig_1", "start": 87037, "stop": 87210, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGGTGAAAAAATATAAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACCAAGTGTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAGTATACGCTTAATAGTAGTTTGGCCAAAGAGAAGGGCAAAAGCCAAAGGGAGGTGCATCTGGGTTCAAATTCAAATCAGTGA", "aa": "MVKKYKLDVPSVSTTMNQVLQELILEQPLTKYTLNSSLAKEKGKSQREVHLGSNSNQ", "aa_hexdigest": "47de3118857a18c40fb9b5dc0f768a5f", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 86911, "stop": 87275, "observations": { "deletion": [ 87235 ], "mutation": [ 87031, 87208 ], "stop": [ 87031, 87208 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 0.9122807017543859, "subject_cov": 0.4297520661157025, "identity": 0.865, "score": 87.4, "evalue": 5.43e-21, "gene_start": 2, "gene_end": 53, "reference_start": 43, "reference_end": 94, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7235772357723578, "score": 154.0, "evalue": 3.67e-50, "description": "Internal stop codon at 87031, 87208. Frameshift due to deletion around 87235. Nonsense mutation around 87031, 87208.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6433.3256, "isoelectric_point": 9.45623874664307 }, "id": "KKNAAMEMLJ_199", "locus": "GCA000027325_00160" }, { "type": "cds", "sequence": "contig_1", "start": 87338, "stop": 87634, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACTGAAGCAACAACGGCAAAAAATGATGCGCCCAGTGTTTCTGGAGGGAGATCATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGAAAGGATCGGTATCTTGTTTGAAAGTAATGGAGAGGCGAGGAATGTTGTTAGCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATACCAAACCACCAACACCTACAACAAGTTAATTGAACCTGACAAGTGA", "aa": "MGPSRAKGRIVVGGVQLKQQRQKMMRPVFLEGDHQIKVINSPSTSTPSKHWKGSVSCLKVMERRGMLLASFLYFQPNKVKSGQYQTTNTYNKLIEPDK", "aa_hexdigest": "374f454e002c78261d2311a411639370", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11122.892199999991, "isoelectric_point": 10.382713890075681 }, "id": "KKNAAMEMLJ_200", "locus": "GCA000027325_00161" }, { "type": "cds", "sequence": "contig_1", "start": 88321, "stop": 88629, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCCTATTGCAGTTTTGCCTTTAGCAGGTATTTTTTTAGGAATCGGTGATGCCATTTCTTCCAATTCATCTGGCATTGTTGGTGTGAAATTTTTTGGTGAATTTATTAAACAAGGTGGTAATGTAGTTTTTGCTAACTTACCTATTTTGTTTGCAGTTGCAATTGCGATCACCTTTTCTCAAGATGCAGGGGTTGCTGGATTTTCTGCTTTTGTTTTTTGGGCCACAATGAACGCGTTTATGAGTTCATTAATTATTCCTGTTGATGCAAATAATACTGCTTCAGGTTATAACATCCTTTATTGA", "aa": "MIPIAVLPLAGIFLGIGDAISSNSSGIVGVKFFGEFIKQGGNVVFANLPILFAVAIAITFSQDAGVAGFSAFVFWATMNAFMSSLIIPVDANNTASGYNILY", "aa_hexdigest": "c509c2615c2ebfb2fb4f53bc0cc62f8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10582.222899999993, "isoelectric_point": 4.225490379333496 }, "id": "KKNAAMEMLJ_201", "locus": "GCA000027325_00162" }, { "type": "cds", "sequence": "contig_1", "start": 89076, "stop": 89333, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAACCATCTAATAAACCGTTTTTAGCCAATTCATCAAAGTTACCTTTACGTGTAAGACCATCAACAAGTTCTTTTCAACTTAGAAAGTTTGTTGATTCTTTACTCATGTATTTTGAAAGTGCAGATGTACTATCTTGTCCTGTTGTAGTAGCTCCATTCATCATTCCACTTGTTCCATTAGCTAAAGCAGCAGGAATAACTCATTTTCCTGGAGTATTAAAATCTTCTCAACCTATCACTCTGGTTACATAA", "aa": "MLEPSNKPFLANSSKLPLRVRPSTSSFQLRKFVDSLLMYFESADVLSCPVVVAPFIIPLVPLAKAAGITHFPGVLKSSQPITLVT", "aa_hexdigest": "b5bfafef228a34c32e7120f9423cc423", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9225.878999999992, "isoelectric_point": 9.743316459655762 }, "id": "KKNAAMEMLJ_202", "locus": "GCA000027325_00163" }, { "type": "cds", "sequence": "contig_1", "start": 89617, "stop": 89823, "strand": "+", "frame": 1, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF73E8" ], "nt": "ATGCTAATTGGAATTCCAGCAGCTGGACTTGCAATGATCTTAGCTGCTCCTAAGGGTAGAAGAAAAGAAGCTAGTTCTATTATTGGTAGTGCTGCATTCACTAGTTTTCTAACAGGGATCACCGAACCTTTTGAATTTACCTTTCTTTTCTTAGCACCATGGTTATTCTATGGTATCCACGCTGTATTAGCTGCAGTAAGCTTTTGA", "aa": "MLIGIPAAGLAMILAAPKGRRKEASSIIGSAAFTSFLTGITEPFEFTFLFLAPWLFYGIHAVLAAVSF", "aa_hexdigest": "c0e42e1ce3e2c51319e018cf78de9d4e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AF73E8", "query_cov": 0.8088235294117647, "subject_cov": 0.9166666666666666, "identity": 0.5820000000000001, "score": 63.5, "evalue": 4.71e-12, "valid": false, "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI0021AF73E8" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF73E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF73E8" ], "product": "PTS transporter subunit EIIC" }, "genes": [], "id": "KKNAAMEMLJ_203", "locus": "GCA000027325_00164" }, { "type": "cds", "sequence": "contig_1", "start": 89827, "stop": 89919, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTATTGAGTGCTAACGTTGGACAAACCTTCTCAGGTTCTTTCATTGACTTTATCTTGTATGGGGCTTTACCTGATGGTAGGGGTTGA", "aa": "MNLLSANVGQTFSGSFIDFILYGALPDGRG", "aa_hexdigest": "3cf8f7a3c4b9e071c17bda66713ca988", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3161.5407000000005, "isoelectric_point": 4.207074546813964 }, "id": "KKNAAMEMLJ_204", "locus": "GCA000027325_00165" }, { "type": "cds", "sequence": "contig_1", "start": 89965, "stop": 90366, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00367.25" ], "nt": "TTGATTTATTTCCCTACCTTCTATTTCTTGACAATTCGCTTTAACTTAGCAACTCCTGGTAGAGGTGGTAAGTTAATTACTAAAAAGGAATATTTAGCAGCAAAAGCAGCTCAAAAAACTGATCAAACTACTAACACTAACTTTAATCAAACCCAAATTGAAGCTGGTATGTTACTAAGAGCTTATGGTGGAAGTGAAAACATTGCTGAATTAGGGGCTTGCATTACTAAATTAAGAGTAACAGTTAAAAACCCTGAACTTGTTAATGAAACTATTATTAAAGACTTGGGAGCAGCTGGGGTAATGCGTACCACTCCAACATTCTTTGTAGCAGTGTTTGGTACTCGAGCTGCTGTTTATAAATCAGCAATGCAAGATATTATCCAAGGCAAAGTAAATTGA", "aa": "MIYFPTFYFLTIRFNLATPGRGGKLITKKEYLAAKAAQKTDQTTNTNFNQTQIEAGMLLRAYGGSENIAELGACITKLRVTVKNPELVNETIIKDLGAAGVMRTTPTFFVAVFGTRAAVYKSAMQDIIQGKVN", "aa_hexdigest": "842393a5bb6a1c6635edb57534e7da7c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00367.25", "name": "PTS_EIIB", "length": 32, "aa_cov": 0.24060150375939848, "hmm_cov": 1.0, "evalue": 1.6571444125164384e-13, "score": 44.932472229003906, "start": 58, "stop": 89 } ], "seq_stats": { "molecular_weight": 14589.788599999985, "isoelectric_point": 9.716110801696775 }, "id": "KKNAAMEMLJ_205", "locus": "GCA000027325_00166" }, { "type": "cds", "sequence": "contig_1", "start": 90373, "stop": 90954, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00358.26" ], "nt": "TTGCAAAAAGTCTTAGATAAAAATGATAGTACTGTTGAAAAACCAGAAATAAAACCAACCCCAGTTTTAAAAGTTCAAGATGAAATTGTGATCCTCTCACCAGTTAATGGCACCTTAAAACCGCTCACCCAAGTTCCTGATGATACCTTCAAAAATCGTTTGGTAGGAGATGGAATTGCTATCTTACCTAGCGATGGGCACTTCAAAGCACCAGGTGATGTGGGTGTGAAAACTGAACTTGCTTTCCCTACTGGTCATGCCTTTATCTTTGATGTTGATGGTGTGAAAGTAATGCTTCACATTGGGATTGATACAGTAAAAATTAATGCTGATAAAAAACCAGGGGAACAACTTGAAGTGTTTGATGTAAAAACAAAACAAGGAGAATACACTAAATTAAAGAGTGAAAGTGTTGTTGAAGTTGATTTAAAGAAACTTAAACGAAAGTATGATCCAATCACTCCTTTCATTGTGATGCAAGAATCACTTGATAACTTCAAGTTGGTGCCAATTCGCCAACGTGGTGAAATTAAAGTTGGCCAACCTTTATTTAAACTAATTTATAAAGATAAGAAGAGTTAA", "aa": "MQKVLDKNDSTVEKPEIKPTPVLKVQDEIVILSPVNGTLKPLTQVPDDTFKNRLVGDGIAILPSDGHFKAPGDVGVKTELAFPTGHAFIFDVDGVKVMLHIGIDTVKINADKKPGEQLEVFDVKTKQGEYTKLKSESVVEVDLKKLKRKYDPITPFIVMQESLDNFKLVPIRQRGEIKVGQPLFKLIYKDKKS", "aa_hexdigest": "8ecbbbf44713947fe194fb2c4651b80b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00358.26", "name": "PTS_EIIA_1", "length": 134, "aa_cov": 0.6839378238341969, "hmm_cov": 0.917910447761194, "evalue": 2.569453557147754e-29, "score": 96.62474822998047, "start": 30, "stop": 161 } ], "seq_stats": { "molecular_weight": 21561.90710000001, "isoelectric_point": 8.857456398010253 }, "id": "KKNAAMEMLJ_206", "locus": "GCA000027325_00167" }, { "type": "cds", "sequence": "contig_1", "start": 91065, "stop": 91157, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATACAAATACTGAAAAACCTGAGTTAGTTTCCCTTAATAAGTTAAGTGAGATGCGCACTAACATCGGGATGGTTAAACGTTATTGA", "aa": "MSDTNTEKPELVSLNKLSEMRTNIGMVKRY", "aa_hexdigest": "6e9dafaa0e44b65bba63ebdcdfba1eed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3486.004500000001, "isoelectric_point": 8.189886283874515 }, "id": "KKNAAMEMLJ_207", "locus": "GCA000027325_00168" }, { "type": "cds", "sequence": "contig_1", "start": 91167, "stop": 91382, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTCTTTATCGAACCTGAACGTAAGCATAATAACGATTTATTGAAGCTTGATCTACAGTACCAAGCGTTAAAAACTGCTTATAACTTCATTAAGGATGTTGTTAAAAATCACGGACAAATCCTTTTTGTTGGAACAAAGAATGATTATGTTAAAAAACTGGTAATTGATATTGCTAAAAGAGTTAATGTTGCATATATTACCCAGCGCTGA", "aa": "MGFFIEPERKHNNDLLKLDLQYQALKTAYNFIKDVVKNHGQILFVGTKNDYVKKLVIDIAKRVNVAYITQR", "aa_hexdigest": "0389f12c2bf01c4493ad346391329ca1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8338.6413, "isoelectric_point": 9.646162605285646 }, "id": "KKNAAMEMLJ_208", "locus": "GCA000027325_00169" }, { "type": "cds", "sequence": "contig_1", "start": 91641, "stop": 91919, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCACTATGCAACACCAACACCAATCCAGAGCTAGTTGACTTTATTATTCCAGCTAATAACCACCAACCCCAAAGTACTTGTTTATTGATGAATTTACTAGCAGATGCGATAGCAGAAGCGAAGGGTTTTGAAACCTTGTATGCTTACAAACCAGATGAACAGATCCAAATTGAAATTCCTCCCAAACAAGAACGCCAAGTTATTAACCGTTCCAATACCAGAAACATCACTAACCAGCGCTTAAACATTAACCGTCAACAACAAGAAACTTTATAG", "aa": "MALCNTNTNPELVDFIIPANNHQPQSTCLLMNLLADAIAEAKGFETLYAYKPDEQIQIEIPPKQERQVINRSNTRNITNQRLNINRQQQETL", "aa_hexdigest": "c4ec16aa3b78c1dcdc7b7e018ba94fdf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10563.82369999999, "isoelectric_point": 5.242680931091308 }, "id": "KKNAAMEMLJ_209", "locus": "GCA000027325_00170" }, { "type": "cds", "sequence": "contig_1", "start": 92257, "stop": 92652, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAAAGTCTTTCACAAGGGTTTTTAGGAATGGTGAATTAATTAGCATTAATGTTAGTGAAGTTGTTGTAGGAGATATTATTTTTGTTGATGCAGGAGATATTATCCCTGCTGATGGCAAATTACTACAGGTTAATAACTTACGTTGTTTGGAAAGCTTTTTAACTGGTGAATCAACTCCAGTTGATAAGACTATTGATAGCAATGAAAAAGCTACTATTCTTGAACAGACAAACTTAGTTTTTTCAGGGGCACAAGTAGTTTATGGTAGTGGCGTTTTTCAAGTGGAAGCAGTTGGGATTAAAACCCAAGTTGGAAAAATTGCTAAAACTGTTGATGATAGTGTAACTAAACTCTCACCCTTACAACAAAAACTAGAGAAGATAGGAAAGTGA", "aa": "MTKSFTRVFRNGELISINVSEVVVGDIIFVDAGDIIPADGKLLQVNNLRCLESFLTGESTPVDKTIDSNEKATILEQTNLVFSGAQVVYGSGVFQVEAVGIKTQVGKIAKTVDDSVTKLSPLQQKLEKIGK", "aa_hexdigest": "26ea0a1df61a9930a7013063e550e6f4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14114.037299999987, "isoelectric_point": 5.132242774963378 }, "id": "KKNAAMEMLJ_210", "locus": "GCA000027325_00171" }, { "type": "cds", "sequence": "contig_1", "start": 92812, "stop": 93084, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTTGCATTAAGTGTGCAGAAACTAACTAAGCAAAAAGCCATTATTAAGTATTTATCAGTAATTGAAACACTTGGATCAGTACAAATTATCTGTACTGATAAAACTGGTACTTTAACCCAAAACCAGATGAAAGTTGTCGATCACTTCTGTTTTAATTCAACAACCCAAACTGATCTAGCAAGAGCATTGTGCTTGTGTAATAATGCTTCTATTTCCAAAGATGCTAATAAAACAGGTGATCCTACTGAAATTGCTCTCTTGGAATGA", "aa": "MIFALSVQKLTKQKAIIKYLSVIETLGSVQIICTDKTGTLTQNQMKVVDHFCFNSTTQTDLARALCLCNNASISKDANKTGDPTEIALLE", "aa_hexdigest": "2e0900c010e0c7cab633e785977486aa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9851.356999999993, "isoelectric_point": 7.646742820739747 }, "id": "KKNAAMEMLJ_211", "locus": "GCA000027325_00172" }, { "type": "cds", "sequence": "contig_1", "start": 93160, "stop": 93654, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGACAGTTGTTGTTCAAAAAGACAACCGCTTCATTGTGATTGTTAAAGGTGCTCCTGATGTGTTATTACCATTATGTAATAACGTTCAAAATGAAGTAAAGAACATTGAAAACTTACTTGATCAAAGTGCTGGTCAAGGCTTGCGTACCTTAGCAGTTGCTTTAAAGGTTTTATATAAGTTTGATCAAAACGATCAGAAGCAAATTGATGAACTTGAAAACAACCTTGAATTCCTTGGGTTTGTTAGTTTACAAGACCCACCAAGAAAAGAAAGTAAGGAAGCGATTTTAGCGTGCAAGAAAGCTAATATAACCCCAATAATGATTACAGGGGATCATCTTAAAACTGCAACTGTAATTGCTAAAGAGTTAGGCATTTTAACTTTAGATAATCAAGCAGTTTTAGGTAGCGAACTAGATGAAAAGAAGATCTTGGATTACAGGGTATTTGCTAGAGTAACTCCCCAACAAAAATTAGCCATTGTTAGTGCTTGA", "aa": "MTVVVQKDNRFIVIVKGAPDVLLPLCNNVQNEVKNIENLLDQSAGQGLRTLAVALKVLYKFDQNDQKQIDELENNLEFLGFVSLQDPPRKESKEAILACKKANITPIMITGDHLKTATVIAKELGILTLDNQAVLGSELDEKKILDYRVFARVTPQQKLAIVSA", "aa_hexdigest": "814d178d2196d3ffee1a69c6a9a21bcf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 122, "aa_cov": 0.7134146341463414, "hmm_cov": 0.8934426229508197, "evalue": 1.2185439559869646e-09, "score": 33.89188766479492, "start": 46, "stop": 162 } ], "seq_stats": { "molecular_weight": 18194.947099999987, "isoelectric_point": 5.883824729919433 }, "id": "KKNAAMEMLJ_212", "locus": "GCA000027325_00173" }, { "type": "cds", "sequence": "contig_1", "start": 93694, "stop": 94134, "strand": "+", "frame": 1, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA", "UniRef:UniRef90_UPI001FB434DA" ], "nt": "GTGAATGACGCACCTGCATTAATCAAGAGTGATGTAGGGTGTTGTATGGGGATTACTGGGGTTGATATTGCAAAAGATGCTAGTGATCTGATTATTAGTGATGATAATTTCGCTACTATAGTAAATGGTATTGAGGAGGGTAGAAAAACTTTTTTAACTTGTAAACGAGTTTTATTAAACCTGTTTTTAACTTCAATTGCAGGAACAGTTGTAGTTTTATTAGGACTATTCATCTTAGGACAAGTTTTTAAAACTAATTTATTACAACAAGGTCATGACTTTCAGGTGTTTAGTCCTACCCAACTGCTAATTATTAACTTGTTTGTTCATGGTTTTCCTGCTGTTGCATTAGCAGTACAACCTGTTAAAGAAAAATTGATGGTAGGTAGTTTTTCTACTAAAAATCTGTTTTACAACCGCCAGGGATTTGATTTAATCTGA", "aa": "MNDAPALIKSDVGCCMGITGVDIAKDASDLIISDDNFATIVNGIEEGRKTFLTCKRVLLNLFLTSIAGTVVVLLGLFILGQVFKTNLLQQGHDFQVFSPTQLLIINLFVHGFPAVALAVQPVKEKLMVGSFSTKNLFYNRQGFDLI", "aa_hexdigest": "b454b620e8b9ec5f78c6aa2b4224652b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 93529, "stop": 94356, "observations": { "mutation": [ 93652, 94132 ], "stop": [ 93652, 94132 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FB434DA", "query_cov": 1.0, "subject_cov": 0.5270758122743683, "identity": 0.8220000000000001, "score": 240.0, "evalue": 8.36e-78, "gene_start": 1, "gene_end": 146, "reference_start": 56, "reference_end": 201, "reference_sequence": "MGILTDERQAILGVDLDPAKIMEYRVFARVTPQQKLEIVNAWKQAGYTVAVTGDGVNDAPALVTSDVGCCMGQTGVDIAKDAADVIISDDNFATIVNGIEQGRKTFLTCKRVLFNLFLTSIAGTIVVLLGLFVLGEVFREQLSKANHNFQVFTPTQLLIINLFVHGFPAVALAIQPVQEKLMLKPFSTKNLFYNRGGFDLIWQSLLLSFLTLLFYSLGMVYAINDPELGKSGDLINRAGATCGFMVLGGSAALNSLNLMVDRPLVATNPKHYGIVXX" }, "paralog": false, "identity": 0.7709090909090909, "score": 425.0, "evalue": 5.48e-153, "description": "Internal stop codon at 93652, 94132. Nonsense mutation around 93652, 94132.", "psc": { "uniref90_id": "UniRef90_UPI001FB434DA", "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FB434DA", "UniRef:UniRef50_UPI001FB434DA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA" ], "product": "HAD-IC family P-type ATPase" } }, "id": "KKNAAMEMLJ_213", "locus": "GCA000027325_00174" }, { "type": "cds", "sequence": "contig_1", "start": 94170, "stop": 94397, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAATAAAAATACCAGTATAGAAGCAAGTGAACCTATTCAAACTAACTTAAAAAACCAAGGGTTTGTCATAAGCAATGGTTTATCTACCATTAGGTTTAATGAGTTTAAAGCAGCACTAGCACCCAAAATAAAAAAACCGCACGTTGATCCAGCACGATTAATTAGATCCCCGCTAGTTTGTAAATCACGGTTATTAATTGCATATATAATTCCTAAGCTATAG", "aa": "MINKNTSIEASEPIQTNLKNQGFVISNGLSTIRFNEFKAALAPKIKKPHVDPARLIRSPLVCKSRLLIAYIIPKL", "aa_hexdigest": "bca55a16b16ca7405203fd8d8eab7927", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8370.879199999996, "isoelectric_point": 10.294843482971189 }, "id": "KKNAAMEMLJ_214", "locus": "GCA000027325_00175" }, { "type": "cds", "sequence": "contig_1", "start": 94535, "stop": 95782, "strand": "+", "frame": 2, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002", "SO:0001217", "UniRef:UniRef50_P47318", "UniRef:UniRef90_P47318" ], "nt": "ATGGCAATATTTAACTTCCTTAAGTTAATTTCACCCAAAAACAGAATTCTCAGTAAGGCAAATAGGATTGCCAGTGAGGTTGAGAGTTATAAAAACTACTACCGTAACTTAACTGATCAACAGTTATTTGAAGAGTCAAATAAACTAGTTGATCTTGTCACTAAGCAAAATTACACCATTCTAGATGTTTGTGTTGCTGCACTTGCTTTAATTAGAGAAGTGGTTTACCGTGAGACTGGTGAATTTGCATATAGGGTGCAGATCATAGGAGCTTTTATTGTTTTAAGTGGTGATTTTGCTGAGATGATGACTGGTGAAGGTAAGACCTTAACCATTGTTTTAGCAGCATACGTTTCTGCACTTGAAAAGCGTGGTGTGCATGTTGTTACTGTTAATGAATATCTAGCTCAAAGGGATGCTAATAATGCAATGAAGATCTTAAAACGGGTTGGGATGAGTGTCGGTTGTAACTTTGCTAATCTCTCCCCTCAGCTAAAACAAGCTGCATTTAATTGCGATGTTACCTACACCACTAACAGTGAACTGGGGTTTGATTATCTTAGAGATAACATGGTCCACAGTTATCAAGATAAGAAGATCAGAGAGTTGCACTTTGCAATAGTTGATGAAGGTGATTCAGTTTTAATTGATGAGGCGCGAACGCCTTTAATTATTTCAGGTCCTAGTAAAAATGAGTTTGGGTTATATGTTGCAGTTGATCGATTTGTTAAATCATTAACTGAACAGGAGTTTAAGATTGACCCTGAATCACGTGCTGCTTCTTTAACTGAACTTGGGATTAAAAAAGCAGAGCAAACATTTAAAAAAGAAAACCTTTTTGCTTTGGAAAACAGTGATCTTTTTCACAAGATCATGAATGGTTTGACTGCTGTGAAAGTTTTTGAACAGGGCAAAGAGTACATTGTTCGTGATGGCAAGGTTTTAATTGTTGATCACTTTACAGGTAGGATATTGGAAGGGAGAAGTTACAGTAATGGCTTACAACAAGCTGTACAAGCCAAAGAATATGTTGAGATAGAACCTGAAAATGTGATAGTAGCTACCATTACCTACCAATCCTTCTTTAGGCTATACAACCGCTTAGCAGCAGTATCAGGTACTGCTTTAACTGAATCAGAGGAGTTTCTCAAGATTTATAACATGGTTGTAGTACCAGTGCCAACTAACCGTCCTAACATCAGAAAAGACCGTTCTGATAGTGTATTTGGTACCCCACAAATTAAGTGA", "aa": "MAIFNFLKLISPKNRILSKANRIASEVESYKNYYRNLTDQQLFEESNKLVDLVTKQNYTILDVCVAALALIREVVYRETGEFAYRVQIIGAFIVLSGDFAEMMTGEGKTLTIVLAAYVSALEKRGVHVVTVNEYLAQRDANNAMKILKRVGMSVGCNFANLSPQLKQAAFNCDVTYTTNSELGFDYLRDNMVHSYQDKKIRELHFAIVDEGDSVLIDEARTPLIISGPSKNEFGLYVAVDRFVKSLTEQEFKIDPESRAASLTELGIKKAEQTFKKENLFALENSDLFHKIMNGLTAVKVFEQGKEYIVRDGKVLIVDHFTGRILEGRSYSNGLQQAVQAKEYVEIEPENVIVATITYQSFFRLYNRLAAVSGTALTESEEFLKIYNMVVVPVPTNRPNIRKDRSDSVFGTPQIK", "aa_hexdigest": "e958cebffacd0c1992c5bdc837f2fc3d", "start_type": "ATG", "rbs_motif": null, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 94535, "stop": 96085, "observations": { "mutation": [ 95780 ], "stop": [ 95780 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47318", "query_cov": 1.0, "subject_cov": 0.5142503097893433, "identity": 1.0, "score": 801.0, "evalue": 1.93e-286, "gene_start": 1, "gene_end": 415, "reference_start": 1, "reference_end": 415, "reference_sequence": "MAIFNFLKLISPKNRILSKANRIASEVESYKNYYRNLTDQQLFEESNKLVDLVTKQNYTILDVCVAALALIREVVYRETGEFAYRVQIIGAFIVLSGDFAEMMTGEGKTLTIVLAAYVSALEKRGVHVVTVNEYLAQRDANNAMKILKRVGMSVGCNFANLSPQLKQAAFNCDVTYTTNSELGFDYLRDNMVHSYQDKKIRELHFAIVDEGDSVLIDEARTPLIISGPSKNEFGLYVAVDRFVKSLTEQEFKIDPESRAASLTELGIKKAEQTFKKENLFALENSDLFHKIMNGLTAVKVFEQGKEYIVRDGKVLIVDHFTGRILEGRSYSNGLQQAVQAKEYVEIEPENVIVATITYQSFFRLYNRLAAVSGTALTESEEFLKIYNMVVVPVPTNRPNIRKDRSDSVFGTPQIKWMAVVKEIKKIHETSRPILIGTANIDDSELLHNLLLEANIPHEVLNAKNHSREAEIVAKAGQKNAVTISTNMAGRGTDIRLGEGVAEMGGLYVLGTERNESRRIDNQLRGRAARQGDKGETKFFISLGDSLFKRFAHDKIERAISKLGNETFDSAFFSKMLSRTQKRVEAINFDTRKNLIDYDHVLASQRELIYKQRDKFLLANDLSEMIDKMLEKFVQQFCDQYRNQKNQNLINHIALAEALNLEMNMQNTINPKVFENMTFDVAVDKTRNLVAKKISDKVNVLTKPIALNRFRDIIITSMDKHWTEHLDSVFKLREGVVLRSMEHTSPLNVYIKETDILFKTMLQKIAQDVIVQIANLTTPDEFDHSLMQANALKKLAAIKADEKSNQEQ" }, "paralog": false, "identity": 0.9961240310077519, "score": 995.0, "evalue": 0.0, "description": "Internal stop codon at 95780. Nonsense mutation around 95780.", "psc": { "uniref90_id": "UniRef90_P47318", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_P47318", "cog_id": "COG0653", "cog_category": "U", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47318", "EC:7.4.2.8", "UniRef:UniRef50_P47318", "COG:COG0653", "COG:U", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_P47318", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47318" ], "product": "Protein translocase subunit SecA" } }, "id": "KKNAAMEMLJ_215", "locus": "GCA000027325_00176" }, { "type": "cds", "sequence": "contig_1", "start": 95783, "stop": 96697, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21090.3" ], "nt": "ATGGCAGTTGTTAAAGAGATAAAAAAGATCCATGAAACTTCTCGACCTATTCTGATTGGAACTGCTAACATAGATGATTCTGAACTCTTACATAATCTGTTACTAGAAGCTAATATTCCCCATGAGGTTTTAAATGCTAAAAACCATTCAAGAGAAGCGGAGATAGTAACTAAAGCAGGACAGAAGAATGCAGTTACTATTTCAACTAACATGGCTGGAAGAGGAACTGATATCCGTTTAGGTGAAGGGGTTGCTGAAATGGGTGGTCTTTATGTATTGGGAACTGAAAGAAATGAGTCAAGAAGGATTGATAACCAACTAAGAGGGAGAGCTGCTAGACAAGGTGATAAAGGGGAAACTAAGTTCTTTATCTCACTAGGTGATTCATTGTTTAAACGTTTTGCTCATGACAAGATTGAAAGAGCGATTAGCAAATTAGGTAATGAAACATTTGACAGTGCCTTCTTTTCCAAAATGTTAAGTAGAACCCAAAAACGGGTGGAAGCAATTAACTTTGACACTAGAAAAAACCTGATTGATTATGACCATGTTCTTGCAAGTCAAAGGGAATTGATTTACAAACAACGTGATAAGTTTTTATTAGCAAACGATTTAAGTGAAATGATCGACAAAATGCTAGAAAAGTTTGTACAACAGTTTTGTGATCAATATAGAAACCAAAAGAACCAAAACTTAATTAATCACATTGCACTAGCAGAAGCTTTAAATCTTGAGATGAACATGCAAAACACCATTAATCCAAAGGTGTTTGAAAACATGACTTTTGATGTTGCTGTTGATAAAACCCGTAACTTAGTAGCTAAAAAGATTAGTGATAAAGTTAATGTTTTGACCAAACCAATTGCTTTAAACAGGTTTCGTGACATTATCATAACTTCGATGGATAAACATTGA", "aa": "MAVVKEIKKIHETSRPILIGTANIDDSELLHNLLLEANIPHEVLNAKNHSREAEIVTKAGQKNAVTISTNMAGRGTDIRLGEGVAEMGGLYVLGTERNESRRIDNQLRGRAARQGDKGETKFFISLGDSLFKRFAHDKIERAISKLGNETFDSAFFSKMLSRTQKRVEAINFDTRKNLIDYDHVLASQRELIYKQRDKFLLANDLSEMIDKMLEKFVQQFCDQYRNQKNQNLINHIALAEALNLEMNMQNTINPKVFENMTFDVAVDKTRNLVAKKISDKVNVLTKPIALNRFRDIIITSMDKH", "aa_hexdigest": "4064bc92404849854f8e045eadb35974", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21090.3", "name": "P-loop_SecA", "length": 81, "aa_cov": 0.26644736842105265, "hmm_cov": 1.0, "evalue": 2.710908502223993e-63, "score": 208.35220336914062, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 34701.39450000003, "isoelectric_point": 9.228987693786618 }, "id": "KKNAAMEMLJ_216", "locus": "GCA000027325_00177" }, { "type": "cds", "sequence": "contig_1", "start": 96707, "stop": 96955, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAGTGTTTTTAAGTTAAGAGAAGGGGTTGTACTTCGTTCTATGGAACATACGAGTCCTTTAAATGTTTACATTAAAGAAACAGATATCCTTTTTAAAACAATGTTGCAAAAGATTGCTCAAGATGTCATTGTGCAAATTGCTAACCTCACAACTCCAGATGAATTTGATCATAGCTTAATGCAAGCCAATGCTTTAAAGAAACTAGCAGCAATTAAAGCAGATGAAAAATCAAACCAAGAGTAA", "aa": "MDSVFKLREGVVLRSMEHTSPLNVYIKETDILFKTMLQKIAQDVIVQIANLTTPDEFDHSLMQANALKKLAAIKADEKSNQE", "aa_hexdigest": "cb6e634dd1f2b81062d3800bf3643a62", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9303.670099999994, "isoelectric_point": 5.623217964172363 }, "id": "KKNAAMEMLJ_217", "locus": "GCA000027325_00178" }, { "type": "cds", "sequence": "contig_1", "start": 96933, "stop": 98903, "strand": "+", "frame": 3, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887", "KEGG:K03702", "RefSeq:WP_010869318.1", "SO:0001217", "UniParc:UPI0000137EB8", "UniRef:UniRef100_P47319", "UniRef:UniRef50_P47319", "UniRef:UniRef90_P47319" ], "nt": "ATGAAAAATCAAACCAAGAGTAATAGTTTATTTCAACTTTCCACTAACTATATACCTACTGGTGATCAACCTGAAGCAATTAAGAAATTATCAGAATTTAAAACTAAGCAGCAGGTTTTATTGGGGGCCACAGGCACAGGTAAAACCTTTACAATTGCTAATGTAATTCAAAACAGCCAACTCCCAACAGTTGTTATTGCTCATAACAAAACCCTAGCAGGTCAACTCTTCAATGAATTAAAGCAACTGTTTCCTAAAAATGCAGTTGAATATTTTATCTCTTACTTTGATTTTTATCAACCTGAAGCTTACTTACCCAGTAAAGGGATCTACATTGAAAAAAGTGCTACAGTCAATGAAGCGATTAAACGCTTAAGAGTCTCAACACTGCATTCACTTTCAACAAGAAAAGATGTTATTGTAGTAGGTTCTGTTGCTAGTATTTATCCCACCTCATCTCCCAGTGATTTTGTTAAGTATTGCTTGTGGTTTGTGGTTGGCAAAGATTATGATTTGAAAACCATTAAAGATAGGTTAGTTAGTCTTAACTATGTTGTTAATAAACAACAATTAACCCCAGGAAAATTTCGCTTTCAGGGTGATGTTTTGGAGGTATTTCCTGGTTACAGTGATGCTTTTGTGATCAGAATCTCCTTTTTTGATACTAAAGTAGAACAAATTTGTCAAATTGACCCACTAACAAATAAGATTTTAAACCAACTCTTTGAGATTAAGATAGGTCCTGCTGATGAATATGTTGTAAACCAATCTGATCTTGATATAGCAATTAAAAATATTAAACAAGAACTTCAGGAACGAGTTAATTATTTCAATAAGCAAAATCTTGTTGAAAGAGCACAACGTTTAGCCACCATTACTAACCATGATCTCAATGATCTGAAGGCTTGGGGATTTTGTAGTGGAGTTGAAAACTATGCTAGACACTTAGAGTTGAGGATGGCTAACTCAACCCCTTACAGTATCTTTGATTATTTTAAGGGGGATTGGTTACTGGTTATTGATGAATCACACCAAACTTTACCGCAACTTAATGGGATGTATAACACTGATCTTTCAAGAAAGCAAAGCTTAATTGATTATGGTTTTCGACTCCCCTCTGCACTTGATAACAGACCGCTCTCATTTGCTGAATTACAACAAAAAATGCAAAAAGTTATTTATGTTTCAGCAACTCCAAGAGATAAAGAGATTAGTTTAAGTCAGAATAATGTCATTGAACAGTTAGTTAGACCAACTTACTTGGTTGATCCTATTATCGTTGTTAAACCAAAAGATAACCAGGTGGAGGATCTCATTGAAGAGATTATCAACCAACGCCAAAACAACACAAGAACATTTGTTACTGTTTTAACTATTAAGATGGCTGAAAACCTCACTGAATACTTAAAGGAACGCAAAATTAAAGTTGCCTATATCCATAAGGACATTAAAGCATTGGAACGTTTATTGTTAATTAATGACCTGAGAAGAGGTGAATATGAGTGTTTAGTTGGGATTAACCTTTTAAGAGAAGGGTTAGATGTCCCTGAAGTTGCTTTAGTTTGTATCTTTGATGCAGATATCCCAGGACTACCTAGGGATGAGAGAAGTTTAATCCAGATTATTGGACGTGCTGCTAGAAATGAACATGGTCGAGTTGTTATGTATGCTAACCATGTTACTGAACAGATGCAAAAAGCCATTGATGAAACCAAAAGAAGAAGAACTGTTCAAATGGAATATAACAAGCTACATAATAAGACACCAAAAACAGTTGTTAAACCCCTTACCTTTGTTCAACCAATCAAATTAAAAGCTAAGAGTAATGCAGAAAAAAATGCTGCATTAATCAAACAATTAACCAAAGAAATGAAGAAAGCAGCAGCTAATCAAAATTATGAACTTGCCATTGAGATTAGAGATTCCATATTTGAATTGGAAAAAGAAATTGGTAGTAAAATTAAAGTATAG", "aa": "MKNQTKSNSLFQLSTNYIPTGDQPEAIKKLSEFKTKQQVLLGATGTGKTFTIANVIQNSQLPTVVIAHNKTLAGQLFNELKQLFPKNAVEYFISYFDFYQPEAYLPSKGIYIEKSATVNEAIKRLRVSTLHSLSTRKDVIVVGSVASIYPTSSPSDFVKYCLWFVVGKDYDLKTIKDRLVSLNYVVNKQQLTPGKFRFQGDVLEVFPGYSDAFVIRISFFDTKVEQICQIDPLTNKILNQLFEIKIGPADEYVVNQSDLDIAIKNIKQELQERVNYFNKQNLVERAQRLATITNHDLNDLKAWGFCSGVENYARHLELRMANSTPYSIFDYFKGDWLLVIDESHQTLPQLNGMYNTDLSRKQSLIDYGFRLPSALDNRPLSFAELQQKMQKVIYVSATPRDKEISLSQNNVIEQLVRPTYLVDPIIVVKPKDNQVEDLIEEIINQRQNNTRTFVTVLTIKMAENLTEYLKERKIKVAYIHKDIKALERLLLINDLRRGEYECLVGINLLREGLDVPEVALVCIFDADIPGLPRDERSLIQIIGRAARNEHGRVVMYANHVTEQMQKAIDETKRRRTVQMEYNKLHNKTPKTVVKPLTFVQPIKLKAKSNAEKNAALIKQLTKEMKKAAANQNYELAIEIRDSIFELEKEIGSKIKV", "aa_hexdigest": "1d881e29d6810a42afa192d2380797cf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137EB8", "ncbi_nrp_id": "WP_010869318.1", "uniref100_id": "UniRef100_P47319", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137EB8", "RefSeq:WP_010869318.1", "UniRef:UniRef100_P47319" ] }, "ips": { "uniref100_id": "UniRef100_P47319", "uniref90_id": "UniRef90_P47319" }, "psc": { "uniref90_id": "UniRef90_P47319", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "uniref50_id": "UniRef50_P47319", "cog_id": "COG0556", "cog_category": "L", "kegg_orthology_id": "K03702", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47319", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47319" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "KKNAAMEMLJ_218", "locus": "GCA000027325_00179" }, { "type": "cds", "sequence": "contig_1", "start": 98906, "stop": 99112, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47320", "UniRef:UniRef90_P47320" ], "nt": "ATGCGACAGTTTATTAAGCTTAGCCTCTTAGTCTTTGTCTTGCTTTTCTTAAGTGAACTTATCTGTAGGTTCTCTTTACGCTTAGTAAATTCTATTAAAGCGAGATATAAATCTTCAGTATTTTCCTACACTGCTTGTTTGCTATTTTTAAAGAGTTTTCAAAACTTTTCTAATGCTTTTCAGAAGTTAGCTAACTGGGTATTTTGA", "aa": "MRQFIKLSLLVFVLLFLSELICRFSLRLVNSIKARYKSSVFSYTACLLFLKSFQNFSNAFQKLANWVF", "aa_hexdigest": "439da34ab80564d5557d144c90f8276c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 98906, "stop": 99319, "observations": { "mutation": [ 99110, 99296 ], "stop": [ 99110, 99296 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47320", "query_cov": 1.0, "subject_cov": 0.49635036496350365, "identity": 1.0, "score": 126.0, "evalue": 6.01e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MRQFIKLSLLVFVLLFLSELICRFSLRLVNSIKARYKSSVFSYTACLLFLKSFQNFSNAFQKLANWVFWFENDVNELLSIFYFNFDQKSEKVDYNFFNGYKVTAQKVVEKEQLLTCKLSDYYRLFRDKTFWFELINN" }, "paralog": false, "identity": 0.9854014598540146, "score": 267.0, "evalue": 3.47e-94, "description": "Internal stop codon at 99110, 99296. Nonsense mutation around 99110, 99296.", "psc": { "uniref90_id": "UniRef90_P47320", "product": "Uncharacterized protein MG074", "uniref50_id": "UniRef50_P47320", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47320", "UniRef:UniRef50_P47320" ] }, "pscc": { "uniref50_id": "UniRef50_P47320", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47320" ], "product": "Uncharacterized protein MG074" } }, "id": "KKNAAMEMLJ_219", "locus": "GCA000027325_00180" }, { "type": "cds", "sequence": "contig_1", "start": 99383, "stop": 101779, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG075", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47321", "UniRef:UniRef90_P47321" ], "nt": "ATGAAATTAAGTACAATAACTACTATCTGCTTGTCAATATCTGGTGCTTTTGGTACTACTGCAATTGCTTTACCAACTACAGTAGCATTGTTAAAAAACCACCAACAGCAAAACACTGAAAAACAACAAAACCCGATTAAAGATATCCGCTTTGGTTTAAACAATGTACAGGTTCCAAATACCATTCCGTTACACCAAACTGTGGTTGAAGTGACCAACAACAAAGCAATTGTTGATTACAAAGATGCACCCCAAAAATTCTTTTTAGCTAAAAGTGCGTTAAATAATAAACTCCAAGTTGAGTTTGATAAGTTCTTGTTAAGAACAGGAGTGATTAATGCTTTAAATGCAGATTTGAAAGAATGGATAGACCAAACATTATTTATCCCCAATCAAAGTTTTTTTGATCTTAGTGCTAATAAACTCAATTTAACTTTGTCAAATCAAAGCGAAGTGAGTTTAGATCTTGAGTTTATTTTTACTAACTTTAGTGATAAAAATCAACCATTAAAACTCCCCTTTGATGGTAGTGTGGTGGTTAATGCTAACGAGTCATACACCTATTCAGTTAAAGCAACACTACAGAAACTAAAAGTATTGACTTATTCAAGAGCAGATCATTCTGTAGGGATTAGTTATGCAATACCAACAGTGAGTTTAAATGGCAAAACTCAAAATGACTTTAGTTTTAACCCCTTTAAAAGTAATATTAACTTTGCTTTCAAAAATGTCTACAATGCTTTAAATCCGTTTGAAGCACAACAATACCTGGTTGGCCAAGGTAAGTTTTTAAACCAAAAAGTTAATGCTGATGATGTTAAAAATGACATTAATAATCACATTGAAACCCAGTTTAATGTTGCTAAAATCACAGCAACTCTTTTAGGAAAAGCTTTTAAGCAGTTTGGTGAACATAAAAATGGTCAACCACTTTCACTTTTAAAAGTACTAAGTGGATTAAACAATGAGTTTAAACAACTCTTTAATTATGTCAGACCTGGTTTAGGTGATTTTGTTAGTGATTTAATCCAAAGCTCCAGTCAATCAAGTAATAAAAAAACTGTTTACCAGCTATTATTTGAAAACAAGACAACAATTATCCACTTGTTGCAAGATCTTAATATTAGTGAACTGAACTCTGTTTTACCAGTTGTTGATATTTTGTTTGAAGGGATTAACAGTGCAGAATCACTATACCAAAGGATCCAATCATTTAAGGATTTAATTGTTCCTGCTTTAAAAGCTGATAAGCAACTTAAGAGCTTAGAAGCGATTATTTTAGCAGTTTTAGACAATCCTAATACCTATGTTTTTGATCTTGTTTACCAAAACAAATCGATTCTGTTTAATTTGTTAAGTGATTTTCTTAAAAACACCGCAAACACTTTACCATTTCTTCAAGAACAGTTTGATATTGTTAACCACTTATTTGCCAATGAAGCGATCTTTGATCTGTTTTCCAATGCTGACTTTGTTGAGAAGATTGCTGATCTTTTTTTAGCAAAACAAAAGGTACAAGAAGTTAATAATGATGGAACTAAATCAACAAAAATTGTTGATTCGATTTTAGTTGCTACCCTTAAGGGTTTAGTTGGTGATCAGCTCAGTTCAATCACTGAACTGTTAAACATCTATATCTTTGAAAATGAATTTTTAAACAGAAATGATAGTAATAGTTCAGTTAAAAAACAACAAACTGATAGTCTAAAAAATCTGTTTAGCGTAATTGGTGATATTTTAAGTGAGACCAATGTTAACAAGATCACTTTACACGCAGTTAAAAATAATGAACTTCTTTCGTTAGTTGAAACAGCTTCAACACTAAAAATTAAACATCTTAATGTTCAATACAAAGTTTTAGTTGATAAGTTTGAATTGAAAAACAGTTTCATTAAGGAACTATTAAATTTTTTCCCAGATACTAAAGATATCACCCCAACTATTAAAAAGGTACTGTTTGAAAGTGAAAATTATAAAACACTCCGTAAGAAGTATGAAAATGAAGGGTTCCCAGGTTATCACTGGGCTAAGTTCATTGTCCCAGGTACCTTCAATTCTGCTGAAAACACTTTCTATTCTGCTATTGATAAAACTAAATCAATTCGCGATCTGTTTGCTGACATGTTATTTGGTAAGAGTTTAGAGAGTGTTAATGACAGTGATAGTTTTATCAAAATTAATGGTTCATTTACATTAAAGTACCACGGTGATAACTTAAATTTACTCCCTAACTACCACTCATTAATCACTAAGAATGTTGGTTATCAAATAGTTAATGTTAATTTTCATATTGATGCAAGATTGCTAACTGCAGAACTACAAAACACGGTATTTTCAAATCCAAAACCAGTTATTAAATCACCTGTTGAACTCTCCAAATCCTTATTTGAAGTTTGA", "aa": "MKLSTITTICLSISGAFGTTAIALPTTVALLKNHQQQNTEKQQNPIKDIRFGLNNVQVPNTIPLHQTVVEVTNNKAIVDYKDAPQKFFLAKSALNNKLQVEFDKFLLRTGVINALNADLKEWIDQTLFIPNQSFFDLSANKLNLTLSNQSEVSLDLEFIFTNFSDKNQPLKLPFDGSVVVNANESYTYSVKATLQKLKVLTYSRADHSVGISYAIPTVSLNGKTQNDFSFNPFKSNINFAFKNVYNALNPFEAQQYLVGQGKFLNQKVNADDVKNDINNHIETQFNVAKITATLLGKAFKQFGEHKNGQPLSLLKVLSGLNNEFKQLFNYVRPGLGDFVSDLIQSSSQSSNKKTVYQLLFENKTTIIHLLQDLNISELNSVLPVVDILFEGINSAESLYQRIQSFKDLIVPALKADKQLKSLEAIILAVLDNPNTYVFDLVYQNKSILFNLLSDFLKNTANTLPFLQEQFDIVNHLFANEAIFDLFSNADFVEKIADLFLAKQKVQEVNNDGTKSTKIVDSILVATLKGLVGDQLSSITELLNIYIFENEFLNRNDSNSSVKKQQTDSLKNLFSVIGDILSETNVNKITLHAVKNNELLSLVETASTLKIKHLNVQYKVLVDKFELKNSFIKELLNFFPDTKDITPTIKKVLFESENYKTLRKKYENEGFPGYHWAKFIVPGTFNSAENTFYSAIDKTKSIRDLFADMLFGKSLESVNDSDSFIKINGSFTLKYHGDNLNLLPNYHSLITKNVGYQIVNVNFHIDARLLTAELQNTVFSNPKPVIKSPVELSKSLFEV", "aa_hexdigest": "f64c882ea177e9b5365b1e3d8e4f0990", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 99383, "stop": 102082, "observations": { "mutation": [ 101777 ], "stop": [ 101777 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47321", "query_cov": 1.0, "subject_cov": 0.779296875, "identity": 1.0, "score": 1538.0, "evalue": 0.0, "gene_start": 1, "gene_end": 798, "reference_start": 1, "reference_end": 798, "reference_sequence": "MKLSTITTICLSISGAFGTTAIALPTTVALLKNHQQQNTEKQQNPIKDIRFGLNNVQVPNTIPLHQTVVEVTNNKAIVDYKDAPQKFFLAKSALNNKLQVEFDKFLLRTGVINALNADLKEWIDQTLFIPNQSFFDLSANKLNLTLSNQSEVSLDLEFIFTNFSDKNQPLKLPFDGSVVVNANESYTYSVKATLQKLKVLTYSRADHSVGISYAIPTVSLNGKTQNDFSFNPFKSNINFAFKNVYNALNPFEAQQYLVGQGKFLNQKVNADDVKNDINNHIETQFNVAKITATLLGKAFKQFGEHKNGQPLSLLKVLSGLNNEFKQLFNYVRPGLGDFVSDLIQSSSQSSNKKTVYQLLFENKTTIIHLLQDLNISELNSVLPVVDILFEGINSAESLYQRIQSFKDLIVPALKADKQLKSLEAIILAVLDNPNTYVFDLVYQNKSILFNLLSDFLKNTANTLPFLQEQFDIVNHLFANEAIFDLFSNADFVEKIADLFLAKQKVQEVNNDGTKSTKIVDSILVATLKGLVGDQLSSITELLNIYIFENEFLNRNDSNSSVKKQQTDSLKNLFSVIGDILSETNVNKITLHAVKNNELLSLVETASTLKIKHLNVQYKVLVDKFELKNSFIKELLNFFPDTKDITPTIKKVLFESENYKTLRKKYENEGFPGYHWAKFIVPGTFNSAENTFYSAIDKTKSIRDLFADMLFGKSLESVNDSDSFIKINGSFTLKYHGDNLNLLPNYHSLITKNVGYQIVNVNFHIDARLLTAELQNTVFSNPKPVIKSPVELSKSLFEVWKTIFENSVNQILKKEYTFKDNLKFFPFKADGSSRLEFDLSKPDQRVIPFAFVDGYQFQLKKELIPNKETKKEANSSPVLKLYDAVKRNDRQYRPNHHHDDLRNYPSLKSQLELILNLGDKLKANNDFIDDTVVNALQYKTSFKSTLKVNSLGIPINLFFFTLWLKFNLEIPIDGSLTLTSVNVVFPYSLYDTSSNEFTRIVDRLNFTDTNFYLKDAFPNFWFVGF" }, "paralog": false, "identity": 0.9988876529477196, "score": 1759.0, "evalue": 0.0, "description": "Internal stop codon at 101777. Nonsense mutation around 101777.", "psc": { "uniref90_id": "UniRef90_P47321", "product": "Uncharacterized protein MG075", "uniref50_id": "UniRef50_P47321", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47321", "UniRef:UniRef50_P47321", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47321" ], "product": "Uncharacterized protein MG075" } }, "id": "KKNAAMEMLJ_220", "locus": "GCA000027325_00181" }, { "type": "cds", "sequence": "contig_1", "start": 102106, "stop": 102399, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED933E1", "UniRef:UniRef90_UPI001ED933E1" ], "nt": "GTGAAATTTAAGCGATCGACTATCCTTGTAAATTCATTTGAACTAGTATCATACAAACTGTATGGGAAAACAACATTAACGGATGTTAATGTTAATGAACCGTCAATTGGAATTTCTAAGTTAAATTTAAGTCACAATGTGAAAAAGAAAAGGTTAATAGGAATTCCTAAACTGTTAACTTTTAGAGTAGATTTAAAACTAGTTTTATATTGCAAAGCATTAACAACAGTGTCATCTATAAAATCATTATTAGCTTTAAGCTTATCACCAAGGTTTAATATAAGTTCTAGTTGA", "aa": "MKFKRSTILVNSFELVSYKLYGKTTLTDVNVNEPSIGISKLNLSHNVKKKRLIGIPKLLTFRVDLKLVLYCKALTTVSSIKSLLALSLSPRFNISSS", "aa_hexdigest": "f30abffff84eb9517c543fefceed5ee2", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED933E1", "query_cov": 1.0, "subject_cov": 0.941747572815534, "identity": 1.0, "score": 180.0, "evalue": 3.25e-57, "valid": true, "uniref50_id": "UniRef50_UPI001ED933E1" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED933E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED933E1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10843.832299999985, "isoelectric_point": 10.297228813171383 }, "id": "KKNAAMEMLJ_221", "locus": "GCA000027325_00182" }, { "type": "cds", "sequence": "contig_1", "start": 102454, "stop": 102870, "strand": "-", "frame": 3, "gene": null, "product": "MPN214 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_009885931.1", "SO:0001217", "UniParc:UPI0000139204", "UniRef:UniRef100_P47322", "UniRef:UniRef50_P47322", "UniRef:UniRef90_P47322" ], "nt": "ATGGTTTTAAATCAAAACAAAAACAGTAATAAAGCTGAGTATGGTGGGATAGTTGTTAGCGTATTTTATCTAATCCTTTTCTTTTTGATCTTAAACATTACTATTTATTTTCACAAGTCAACCAATTTTACAGTGGTTGTTAAAAATTCAGTACTAACTAGCTTTTTTGTTAACTTATTACTTGTTTGTTTACAGGGGATATTCCGTTTAAAAACTTGTGATGGAATGCGGTATGAAATTAGTAAGTTTAACAGATATCTTAAGTTAGGTAGTGTTTATGCAAAACCATTAGTTAGTTTTAATCAATATCAAGATCAAAGTGCAACTTACAGACAAAAAACTAGTGGGTTTTGGTGGATGAACTTGATTGTTTATCTAGTTGGTAGTTTAGTTAGTGGTTTAGTTTCATTACTCTAG", "aa": "MVLNQNKNSNKAEYGGIVVSVFYLILFFLILNITIYFHKSTNFTVVVKNSVLTSFFVNLLLVCLQGIFRLKTCDGMRYEISKFNRYLKLGSVYAKPLVSFNQYQDQSATYRQKTSGFWWMNLIVYLVGSLVSGLVSLL", "aa_hexdigest": "840fe12a91719bb65803129e7947e092", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139204", "ncbi_nrp_id": "WP_009885931.1", "uniref100_id": "UniRef100_P47322", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139204", "RefSeq:WP_009885931.1", "UniRef:UniRef100_P47322" ] }, "ips": { "uniref100_id": "UniRef100_P47322", "uniref90_id": "UniRef90_P47322" }, "psc": { "uniref90_id": "UniRef90_P47322", "product": "MPN214 family protein", "uniref50_id": "UniRef50_P47322", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47322" ], "product": "Uncharacterized protein MG076" }, "genes": [], "id": "KKNAAMEMLJ_222", "locus": "GCA000027325_00183" }, { "type": "cds", "sequence": "contig_1", "start": 103104, "stop": 103304, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAGTATATTCTTAAACGATTAGGACTAGCAGTAGTTGCGATGTTTATCGTAATGTCTATAGTCTTCTTTTTAGTGAACGCTACTGGTAATGTTCCCTTGTCAGCCACTTCTGCAAGAGATATTGCTGCAGTGCAAGCACAACTACAAGAGTTTGGGTTTAATGACCCTATTATAGTTAGGTATTTTCGCTATTGA", "aa": "MFKYILKRLGLAVVAMFIVMSIVFFLVNATGNVPLSATSARDIAAVQAQLQEFGFNDPIIVRYFRY", "aa_hexdigest": "cf3392981ec65efeae148f757cfeba09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7446.7975000000015, "isoelectric_point": 9.815069770812986 }, "id": "KKNAAMEMLJ_223", "locus": "GCA000027325_00184" }, { "type": "cds", "sequence": "contig_1", "start": 103380, "stop": 104123, "strand": "+", "frame": 3, "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "db_xrefs": [ "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75554", "UniRef:UniRef90_P47323" ], "nt": "GTGTTTGCAAGAGTACCAAATACCTTATATGTGGTTTTAATCTCTTTTTTAATTGGTTCATTGCTAGGGATCTTTTTAGGGATGGTTTCAGGATTGAATAGAGGGAAGTTTTTAGATGCAGCAATTAATGTGTTGGTAGTTTTATTTGTATCTATTCCTTCATTTGTAGTGGGATTAGGGTTACTTAAACTAGCAGGATTTTTAAATCTACCACCACGGTTTATTAACTTTGATGATGCTTTTTTTAGCTTTGATCGTTTCTTGCTTGCATCAATTATCCCGATCCTTTCATTGGTCTTCTATTCATCAGCTGCTTTTACATACAGGATTAGAAATGAGGTGGTGGAAGTGATGAATCAAGACTATATTAAAACTGCAAAAAGTAAGGGACTTGGGATGTTTGCTGTAGCTAGGTATCATATCTTTAGAAACTCGATTATTCCTTCTATTCCCTTGTTTGTATTTGGAATCTCAGGTGCTTTTTCAGGTGGATTTATTATTGAGTCTTTGTTTGGAGTACAAGGGGTATCTAGGATCTTAATTGATTCAGTGCAAGTTAATGAAACTAACATGGTAATGTTTAATATCTTGTTTATCCAAGGGATTCCCTTATTAGCAAGTGTCTTTATTGAATTTATCTATGTTTTAGTTGATCCTAGAATTAGGATTGCAAATAGTTCTAATGTTAGCTTATTAACTAAGTTAAAGTTCTTAAGTTCAAGACACCAATGGTTAATGAAGTGA", "aa": "MFARVPNTLYVVLISFLIGSLLGIFLGMVSGLNRGKFLDAAINVLVVLFVSIPSFVVGLGLLKLAGFLNLPPRFINFDDAFFSFDRFLLASIIPILSLVFYSSAAFTYRIRNEVVEVMNQDYIKTAKSKGLGMFAVARYHIFRNSIIPSIPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQVNETNMVMFNILFIQGIPLLASVFIEFIYVLVDPRIRIANSSNVSLLTKLKFLSSRHQWLMK", "aa_hexdigest": "b7c521ba66acfb3ac96f6e00bcf2a0b3", "start_type": "GTG", "rbs_motif": null, "genes": [ "oppB" ], "truncated": "both", "pseudogene": { "start": 103104, "stop": 104327, "observations": { "mutation": [ 103302, 104121 ], "stop": [ 103302, 104121 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47323", "query_cov": 0.9959514170040485, "subject_cov": 0.6044226044226044, "identity": 1.0, "score": 440.0, "evalue": 5.17e-153, "gene_start": 2, "gene_end": 247, "reference_start": 94, "reference_end": 339, "reference_sequence": "MFKYILKRLGLAVVAMFIVMSIVFFLVNATGNVPLSATSARDIAAVQAQLQEFGFNDPIIVRYFRYWAKLFSFQADALGIYYANPNQTIGEIVFARVPNTLYVVLISFLIGSLLGIFLGMVSGLNRGKFLDAAINVLVVLFVSIPSFVVGLGLLKLAGFLNLPPRFINFDDAFFSFDRFLLASIIPILSLVFYSSAAFTYRIRNEVVEVMNQDYIKTAKSKGLGMFAVARYHIFRNSIIPSIPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQVNETNMVMFNILFIQGIPLLASVFIEFIYVLVDPRIRIANSSNVSLLTKLKFLSSRHQWLMKWNKINSDNAQNIVFNSPLHHQLLELNAIDYKTKTVQLTTEQKTALNISATANFILLGNKCLKLKTIHG" }, "paralog": false, "identity": 0.995085995085995, "score": 761.0, "evalue": 2.91e-282, "description": "Internal stop codon at 103302, 104121. Nonsense mutation around 103302, 104121.", "psc": { "uniref90_id": "UniRef90_P47323", "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "uniref50_id": "UniRef50_P75554", "cog_id": "COG0601", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47323", "UniRef:UniRef50_P75554", "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75554", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75554" ], "product": "Oligopeptide transport system permease protein OppB" } }, "id": "KKNAAMEMLJ_224", "locus": "GCA000027325_00185" }, { "type": "cds", "sequence": "contig_1", "start": 104157, "stop": 104327, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAACTCGCCACTGCACCACCAGCTATTAGAACTCAATGCAATTGATTACAAAACAAAAACAGTTCAACTAACAACTGAACAAAAAACTGCTCTCAATATCAGTGCAACTGCTAACTTTATCTTACTTGGTAACAAGTGTTTAAAACTCAAAACAATCCATGGATAG", "aa": "MFNSPLHHQLLELNAIDYKTKTVQLTTEQKTALNISATANFILLGNKCLKLKTIHG", "aa_hexdigest": "ae026fa01f44486324269dbf017cbde2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6279.291399999998, "isoelectric_point": 9.361599159240722 }, "id": "KKNAAMEMLJ_225", "locus": "GCA000027325_00186" }, { "type": "cds", "sequence": "contig_1", "start": 104320, "stop": 104805, "strand": "+", "frame": 1, "gene": "oppC", "product": "Oligopeptide transport system permease protein OppC", "db_xrefs": [ "COG:COG1173", "COG:EP", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75553", "UniRef:UniRef90_P47324" ], "nt": "ATGGATAGAAATAAAAGTTTTGACCCTAACTTATTTAAAAGGGTTGATATCAACTTATTAAAGCGAAATGATCAGCTTATTGGTAAACCAACTACCAATTCAATAGAAATTATCAAGCGCTTGTTTCAAAACAAGTGGGCCATCTTATTTTTTCTTTTAATAGTTGTTATTGTGCTATTAGCAATTATTGTGCCTTTAACTTCCCCTTTTTCAGCAGTAACTCCTGTTTCAACCAATGCCTTAGCACAAAATCTACCACCACGGTACTTATGGCATAAACCAGGTGACATTTTAGTTCATAAGATTACAGCAAGATCAATTGCTGAAATCTCTCAAGCTAGTGGAGTTTTAGTAGGAACATTACCTAGTGCAAATAGTAATCCCTTAGCAACTAATGTCCAGTATGATATTGCTCCTTTTCAACTCCAAGAATTGCGTAATTATTTCCCTTTATTGGGGACTAATGGACTTGGGATTGATATTTGA", "aa": "MDRNKSFDPNLFKRVDINLLKRNDQLIGKPTTNSIEIIKRLFQNKWAILFFLLIVVIVLLAIIVPLTSPFSAVTPVSTNALAQNLPPRYLWHKPGDILVHKITARSIAEISQASGVLVGTLPSANSNPLATNVQYDIAPFQLQELRNYFPLLGTNGLGIDI", "aa_hexdigest": "1b9067c6ad8d61f120903ceacc121ac9", "start_type": "ATG", "rbs_motif": null, "genes": [ "oppC" ], "truncated": "3-prime", "pseudogene": { "start": 104320, "stop": 105108, "observations": { "mutation": [ 104803, 104815, 104839, 104980, 105070, 105076 ], "stop": [ 104803, 104815, 104839, 104980, 105070, 105076 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47324", "query_cov": 1.0, "subject_cov": 0.42819148936170215, "identity": 1.0, "score": 301.0, "evalue": 2.66e-100, "gene_start": 1, "gene_end": 161, "reference_start": 1, "reference_end": 161, "reference_sequence": "MDRNKSFDPNLFKRVDINLLKRNDQLIGKPTTNSIEIIKRLFQNKWAILFFLLIVVIVLLAIIVPLTSPFSAVTPVSTNALAQNLPPRYLWHKPGDILVHKITARSIAEISQASGVLVGTLPSANSNPLATNVQYDIAPFQLQELRNYFPLLGTNGLGIDIWTLLWASVAKSLWIAVVVAIIAMVFGTIYGAVAGSFVGHMADNIMSRIIEIIDIVPSILWIIVLGATFRFGGVKQFDDSVVIFTLIFVFWTWPATTTRIYILKNKDTEYIQAAKTLGAHQIRIIFVHMLPVVFGRLAVVFVSLIPAVIGYEASLVFLGLKPATDIGLGALLNQVTSSDNVALILSSIVSFAVLTVAARTFANALNDAIDPRVVKR" }, "paralog": false, "identity": 0.9732824427480916, "score": 418.0, "evalue": 1.2e-148, "description": "Internal stop codon at 104803, 104815, 104839, 104980, 105070, 105076. Nonsense mutation around 104803, 104815, 104839, 104980, 105070, 105076.", "psc": { "uniref90_id": "UniRef90_P47324", "gene": "oppC", "product": "Oligopeptide transport system permease protein OppC", "uniref50_id": "UniRef50_P75553", "cog_id": "COG1173", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47324", "UniRef:UniRef50_P75553", "COG:COG1173", "COG:EP", "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75553", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75553" ], "product": "Oligopeptide transport system permease protein OppC" } }, "id": "KKNAAMEMLJ_226", "locus": "GCA000027325_00187" }, { "type": "cds", "sequence": "contig_1", "start": 104869, "stop": 104982, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGTTTGGAACCATTTATGGAGCGGTTGCTGGAAGCTTTGTTGGACATATGGCTGATAACATTATGAGTAGGATCATTGAGATTATTGATATAGTCCCTTCTATTCTTTGA", "aa": "MVFGTIYGAVAGSFVGHMADNIMSRIIEIIDIVPSIL", "aa_hexdigest": "4936c367a942cc35c6979e56c0d499a9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3951.6727999999994, "isoelectric_point": 4.535092353820801 }, "id": "KKNAAMEMLJ_227", "locus": "GCA000027325_00188" }, { "type": "cds", "sequence": "contig_1", "start": 105106, "stop": 105450, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAACAAAGATACAGAGTACATCCAAGCAGCTAAGACCCTAGGGGCACACCAAATCAGAATTATCTTTGTTCATATGTTACCTGTTGTATTTGGGAGATTAGCTGTTGTGTTTGTTAGTTTAATCCCAGCAGTTATTGGTTATGAAGCTTCCTTAGTTTTCCTTGGGTTAAAACCAGCTACTGATATTGGCTTAGGGGCACTTTTAAACCAAGTAACTTCAAGTGATAATGTAGCTTTAATCTTAAGTTCGATTGTTAGCTTTGCAGTTTTAACAGTAGCAGCTAGAACATTTGCTAATGCTTTAAATGATGCAATTGACCCTAGGGTTGTAAAACGATAA", "aa": "MKNKDTEYIQAAKTLGAHQIRIIFVHMLPVVFGRLAVVFVSLIPAVIGYEASLVFLGLKPATDIGLGALLNQVTSSDNVALILSSIVSFAVLTVAARTFANALNDAIDPRVVKR", "aa_hexdigest": "b1cfb3aa042724869febbd9f66897e22", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12190.27969999998, "isoelectric_point": 9.59697322845459 }, "id": "KKNAAMEMLJ_228", "locus": "GCA000027325_00189" }, { "type": "cds", "sequence": "contig_1", "start": 105452, "stop": 106564, "strand": "+", "frame": 2, "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "db_xrefs": [ "COG:COG0444", "COG:EP", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47325", "UniRef:UniRef90_P47325" ], "nt": "ATGGCACTTAAAAGAAGTAATTTCTTTGTTGATAAAGACCAACAACTAAAGGATAATTTGATCTTAGACATCACTGATTTACATGTTAACTTTAAGGTTAAAGATGGGATCTTACATGCTGTTAGAGGGATTGATCTTAAGGTAGAGAGGGGTAGTATTGTAGGGATTGTAGGTGAATCAGGCAGCGGTAAATCAGTGAGTGTTAAATCAATTATTGGTTTTAATGACAATGCACAAACTAAAGCCAAACTGATGAACTTTAAAAACGTTGATATTACCAAACTAAAGAAACACCAGTGGAAGTATTATAGAGGGACATATGTCTCTTATATTTCCCAAGACCCATTGTTTTCTCTAAACCCAACAATGACGATAGGAAAACAAGTAAAAGAAGCGATTTATGTGGCTTCAAAAAGAAGGTATTTCCAAGCTAAATCAGACTTAAAATTTGCTTTATCAAATAAGGAGATTGACAAAAAAACTTATAAAAGTAAACTAAAAGAGATCAAACAAACCTACCAACAAAAAATAAAACCTATCAATGTAGAGAAAAAAACCTTAGAGATCCTGCAGTTCATTGGTATTAATGATGCCAAGAAACGTTTAAAGGCATTCCCAAGTGAGTTTTCAGGAGGGATGAGACAGAGAATTGTGATTGCTATTGCAGTAGCAACTGAACCTGATTTAATTATTGCTGATGAACCTACTACTGCACTTGATGTAACTATTCAAGCTAAGGTATTAACTTTAATTAAACAACTCCGTGATCTACTTAATATCACTATTATCTTTATTAGTCACAATATCTCTTTAATTGCTAATTTCTGTGACTTTGTTTATGTTATGTATGCAGGGAAAATTGTAGAACAGGGTCTGGTTGAAGAGATCTTTACAAATCCACTCCATCCCTATACATGGGCATTGATTTCTTCAATTCCTGAACAGAAAGATAAAAACAAACCACTAACTTCTATCCCTGGAGTTATTCCTAACATGTTAACCCCACCAAAGGGTGATGCTTTCGCTAGTAGAAACCAATATGCTCTAGCAATTGACTTTGAATACCATCCACCCTTTTTTGAAGTTACTAAAACCCATAAAGCAGCAACTTGA", "aa": "MALKRSNFFVDKDQQLKDNLILDITDLHVNFKVKDGILHAVRGIDLKVERGSIVGIVGESGSGKSVSVKSIIGFNDNAQTKAKLMNFKNVDITKLKKHQWKYYRGTYVSYISQDPLFSLNPTMTIGKQVKEAIYVASKRRYFQAKSDLKFALSNKEIDKKTYKSKLKEIKQTYQQKIKPINVEKKTLEILQFIGINDAKKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKVLTLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGKIVEQGLVEEIFTNPLHPYTWALISSIPEQKDKNKPLTSIPGVIPNMLTPPKGDAFASRNQYALAIDFEYHPPFFEVTKTHKAAT", "aa_hexdigest": "b87f297b52a9b1034127f2a9af8514c8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47325", "query_cov": 1.0, "subject_cov": 0.9203980099502488, "identity": 1.0, "score": 706.0, "evalue": 3.85e-256, "valid": true, "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P47325", "cog_id": "COG0444", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P47325", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47325" ], "product": "Oligopeptide transport ATP-binding protein OppD" }, "genes": [ "oppD" ], "id": "KKNAAMEMLJ_229", "locus": "GCA000027325_00190" }, { "type": "cds", "sequence": "contig_1", "start": 106660, "stop": 107547, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGAAAACCAAAACACAAAAAAACCACTTGTTAATGTTAAGGCTTTGAGCATGATGTTCAAGGTCAGAGGAACTCTTTTTAAAGCCCTTGATGAAATTGGTTTTACTGTTAATGAAGGGGACTTCTTTGGGGTTATTGGTGAGAGTGGTAGTGGTAAATCAACCACGGGAAAATGTTTGATTAGATTAAACATTCCTAGTGGTGGAAAGATTGAGATTGCCAACCACTTACTCTCAGGAAAAAAACTTACTAAAGAGAATAACCAGTGGTTAAAACAAAACATCCAAATGGTGTTTCAAGACCCTTATTCATCTATTAACCCTACTAAAAATGTGCTAACTGTGATTTCAGAACCGCTGGTAATTAGTAAAACTGTTTTTGGGGAAACAAAACAATACTTAAAGAGTTTGCAAAAGCTCTCTTTTAAAGTAAAGAAAACATTGTTAAGGAATGATATTGAACTTGAAACCAAGTTTCACAATAACTTTTTTAAAACCGTTATTAAGCAAATTAATGAATCATTGTTTAACTTTGAAGATCTTGATTACAAGGATTTAAAACCATCACATTTAAGGCAAAGAATCATAAATGAAACAGATAAATTCATTGAAAAAATTAGAAGTGAGTTTGCCCTTTTTTATGATTTTTATGCTAACCAATCAGTACCCTTGCAAAAGGCATTAGATGATGCGAATTCCTCTTTAACACCATCTAGTGTTATTGAGTTAAAAAACCAGTTAAAAGCATTACAAAAACAAGCAAAGATTTCAAAGGCAGCATGGGATATTTTACAAGCCCTAAAGCAAAACCAAAAGGAGTTGAAAGATTATGAAAATTATGTCCATTTTGAACTCCAAAAAAAGCCACGAATCTATCTTAATACCTGA", "aa": "MENQNTKKPLVNVKALSMMFKVRGTLFKALDEIGFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKIEIANHLLSGKKLTKENNQWLKQNIQMVFQDPYSSINPTKNVLTVISEPLVISKTVFGETKQYLKSLQKLSFKVKKTLLRNDIELETKFHNNFFKTVIKQINESLFNFEDLDYKDLKPSHLRQRIINETDKFIEKIRSEFALFYDFYANQSVPLQKALDDANSSLTPSSVIELKNQLKALQKQAKISKAAWDILQALKQNQKELKDYENYVHFELQKKPRIYLNT", "aa_hexdigest": "f5dd3d79ad7821b0b22cc203ec96dfe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 112, "aa_cov": 0.37966101694915255, "hmm_cov": 0.8303571428571429, "evalue": 9.941132323181529e-15, "score": 50.444705963134766, "start": 30, "stop": 141 } ], "seq_stats": { "molecular_weight": 33933.77400000002, "isoelectric_point": 9.508587074279784 }, "id": "KKNAAMEMLJ_230", "locus": "GCA000027325_00191" }, { "type": "cds", "sequence": "contig_1", "start": 107590, "stop": 108009, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGCTTACTGATGATATCTTTGCTTTTTCATATAACAGTATGGTTGACAAGAAAAGAAACTTGGTTTTAATTCTTTCTAAATACTATAAGCTGTTACCTTATTTCTATGACCAATCAGTATTTGATAATGCTGATCAATTTGATGAAATTGCTAACCTTATCTTTTTTGATTTAGTTGAAACATTGCTTGGTGTAACTAGTTTATTTAATGATGCATTAGCAGCTGATAAAGTCCCACTAATTAAGTTTGCTAAGTTCTTAAATAAGTTATGTGACTTGCGCTTTTTAACCTTAAAAAAGAGCTTTAAAAAAACAAGAGTAAGTTGTAGCTTTAGTTTTAACAGTGAACCTGAAATCTTGTTTGCCAACAGCTGCTATGATTTGCAACAAATGCCTCAAATCATTAAACCCTTTTGA", "aa": "MQLTDDIFAFSYNSMVDKKRNLVLILSKYYKLLPYFYDQSVFDNADQFDEIANLIFFDLVETLLGVTSLFNDALAADKVPLIKFAKFLNKLCDLRFLTLKKSFKKTRVSCSFSFNSEPEILFANSCYDLQQMPQIIKPF", "aa_hexdigest": "e00889b64f6c127ba3f354341623de78", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16205.699899999976, "isoelectric_point": 6.218492698669433 }, "id": "KKNAAMEMLJ_231", "locus": "GCA000027325_00192" }, { "type": "cds", "sequence": "contig_1", "start": 108073, "stop": 109179, "strand": "+", "frame": 1, "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "db_xrefs": [ "COG:COG4172", "COG:Q", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75551", "UniRef:UniRef90_P47326" ], "nt": "ATGATTGCAAATTACATTACCAAAGCTTTTGAAATTAAAAAAACTATTGATGAAAAACTAAGGGAGTTTAAACAACAAAATTTAGCTTTAAAAAAAGCTTATTCAGCTAACAAGAAAAGTGAGGCAAACAAAGCTTCCATTAATGAGTTAAAAGTCAATTTAAAAACACTTAAAAAACAGCTTAAACAAGAGAAAAATACTACTAAAAAACAATCAAAAAAGGAATTAAAACCACTTTTAAAAGAACACCATACTGCTTTAAAACTCCATGATGAGTTTAACCATGATTTACGCAAGTGGTTCAAAAAACTTAACTTTATGGTTAAGAAATACAACCGACTGGAAAACAGCCAGAAAAAGTTTTGTTTAGTTAAAAAGTTAAAAGCGCTTTTCAAAAAACAGGATGAAACACTGCAAAGTGAATTAAGACCAAAACTAAAAACATTTGGTGTAATTAACTTTGAGTACAAACGTGCAGTCAAAGAGTCCAATGTCTTTCGATTGGTGCATTTTGCTAAAAATATCTTTAAACCATTCTTGTTTTTTAACCTCACCAAGATTTTTATGAGAAATAAGGTCTATGAAGCACTTGATAGTGTTGGTTTAAAAAGAGAACATGCTTACAGATACCCCCATGAATTTTCAGGCGGACAAAGACAAAGAATTGCTATTGCCCGTGCTTTAATCACTAAACCCAAACTGATTATTGCAGATGAATTGATTAGTGCACTTGATGTTTCTATCCAAGCCCAAGTTATTAACATCTTGAAAGACTTGGCTAAAAAACACAACTTAACTGTGCTTTTCATTGCCCATGATTTATCAATGGTGCAAACTGTTTGTAACCGTTTGATCATTATGCATAGGGGCAAGATTGTTGAACGGGGCAGTGTGGATGAGATCTTTTCAAATCCAGTTCATCCCTACACCCGTTCCCTAATAAAAGCATCTCCTAAGTTAAGCAAAATCAATGTTGATCTCGCTTCTTTTGATGAAAACTTCACTTATGATAGTGATTATTCACTAACCAATATGCCCTTTTATATTAAAGTTCCTAACAGTGAAGAACATGAACTTTACTGTACTCAAAAGCAATTTGATAGTTGA", "aa": "MIANYITKAFEIKKTIDEKLREFKQQNLALKKAYSANKKSEANKASINELKVNLKTLKKQLKQEKNTTKKQSKKELKPLLKEHHTALKLHDEFNHDLRKWFKKLNFMVKKYNRLENSQKKFCLVKKLKALFKKQDETLQSELRPKLKTFGVINFEYKRAVKESNVFRLVHFAKNIFKPFLFFNLTKIFMRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSVDEIFSNPVHPYTRSLIKASPKLSKINVDLASFDENFTYDSDYSLTNMPFYIKVPNSEEHELYCTQKQFDS", "aa_hexdigest": "e30e96951f90734f5bf7ef8358b68b75", "start_type": "ATG", "rbs_motif": null, "genes": [ "oppF" ], "truncated": "both", "pseudogene": { "start": 107773, "stop": 109206, "observations": { "mutation": [ 108007, 109177 ], "stop": [ 108007, 109177 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47326", "query_cov": 1.0, "subject_cov": 0.4339622641509434, "identity": 1.0, "score": 656.0, "evalue": 1.21e-229, "gene_start": 1, "gene_end": 368, "reference_start": 472, "reference_end": 839, "reference_sequence": "MENQNTKKPLVNVKALSMMFKVRGTLFKALDEIGFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKIEIANHLLSGKKLTKENNQWLKQNIQMVFQDPYSSINPTKNVLTVISEPLVISKTVFGETKQYLKSLQKLSFKVKKTLLRNDIELETKFHNNFFKTVIKQINESLFNFEDLDYKDLKPSHLRQRIINETDKFIEKIRSEFALFYDFYANQSVPLQKALDDANSSLTPSSVIELKNQLKALQKQAKISKAAWDILQALKQNQKELKDYENYVHFELQKKPRIYLNTWLLTTKSYIKDSKQNMQLTDDIFAFSYNSMVDKKRNLVLILSKYYKLLPYFYDQSVFDNADQFDEIANLIFFDLVETLLGVTSLFNDALAADKVPLIKFAKFLNKLCDLRFLTLKKSFKKTRVSCSFSFNSEPEILFANSCYDLQQMPQIIKPFWEKLFNEQNYQKIIDSVSRLNVMIANYITKAFEIKKTIDEKLREFKQQNLALKKAYSANKKSEANKASINELKVNLKTLKKQLKQEKNTTKKQSKKELKPLLKEHHTALKLHDEFNHDLRKWFKKLNFMVKKYNRLENSQKKFCLVKKLKALFKKQDETLQSELRPKLKTFGVINFEYKRAVKESNVFRLVHFAKNIFKPFLFFNLTKIFMRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSVDEIFSNPVHPYTRSLIKASPKLSKINVDLASFDENFTYDSDYSLTNMPFYIKVPNSEEHELYCTQKQFDSWIKEATPIN" }, "paralog": false, "identity": 0.9958071278825996, "score": 885.0, "evalue": 4.2e-322, "description": "Internal stop codon at 108007, 109177. Nonsense mutation around 108007, 109177.", "psc": { "uniref90_id": "UniRef90_P47326", "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P75551", "cog_id": "COG4172", "cog_category": "Q", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47326", "EC:7.4.2.6", "UniRef:UniRef50_P75551", "COG:COG4172", "COG:Q", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75551", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75551" ], "product": "Oligopeptide transport ATP-binding protein OppF" } }, "id": "KKNAAMEMLJ_232", "locus": "GCA000027325_00193" }, { "type": "cds", "sequence": "contig_1", "start": 109262, "stop": 109675, "strand": "+", "frame": 2, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180", "KEGG:K02867", "RefSeq:WP_009885638.1", "SO:0001217", "UniParc:UPI0000165F74", "UniRef:UniRef100_P47327", "UniRef:UniRef50_P47327", "UniRef:UniRef90_P47327" ], "nt": "ATGGCTAAAAAAACAGTTACAAGAATCGCTAAGATTAACCTAATTGGCGGACAAGCAAAACCTGGCCCTGCGCTTGCTTCTGTAGGGATTAATATGGGTGAGTTTACCAAACAATTTAATGAAAAAACCAAGGATAGACAAGGTGAAACGATCCCTTGTATAATCACTGCTTTTAACGATAAATCATTTACTTTTGTCTTAAAAACTACCCCTGTTAGTAACTTAATTAAACAAGCTGCTAAACTAGAAAAAGGTGCTAAAAATGCAAAAACTATTGTTGGAAAAATCTCCTTACAACAAGCTAAGGAGATTGCGCAATACAAGTTAGTTGATCTTAATGCTAACACAGTTGAAGCAGCATTAAAAATGGTGTTAGGTACAGCTAAACAGATGGGAATAGAGGTAACTGATTAA", "aa": "MAKKTVTRIAKINLIGGQAKPGPALASVGINMGEFTKQFNEKTKDRQGETIPCIITAFNDKSFTFVLKTTPVSNLIKQAAKLEKGAKNAKTIVGKISLQQAKEIAQYKLVDLNANTVEAALKMVLGTAKQMGIEVTD", "aa_hexdigest": "19bb1803ec9bd00a603f99a2e03a8a04", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F74", "ncbi_nrp_id": "WP_009885638.1", "uniref100_id": "UniRef100_P47327", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F74", "RefSeq:WP_009885638.1", "UniRef:UniRef100_P47327" ] }, "ips": { "uniref100_id": "UniRef100_P47327", "uniref90_id": "UniRef90_P47327" }, "psc": { "uniref90_id": "UniRef90_P47327", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_P47327", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P47327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47327" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "KKNAAMEMLJ_233", "locus": "GCA000027325_00194" }, { "type": "cds", "sequence": "contig_1", "start": 109675, "stop": 109992, "strand": "+", "frame": 1, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "COG:COG0081", "COG:J", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843", "KEGG:K02863", "SO:0001217", "UniRef:UniRef50_P47328", "UniRef:UniRef90_P47328" ], "nt": "ATGAAAAAACTATCAAAAAGGATGCAAGCTGTTACCAAGCTCATTGATAAAAACAAACTTTATCCTATCCAAGAAGCATTTGAATTAATTAAAAAAACAGCAATTACTAAGTTTGTCAGTTCAGTTGATATTGCTGTTAGTTTAAACCTTGATACTACTAAAGCTGAACAACAGTTAAGAGGTGCAATTGCTTTTCCTTTTAGTATTGGTAAATCTATCAGAATTTTAGCTATCACTGATGATGAGAAAAAAGCTAGTGAAGCAGGTGCTGATTTTGTTGGTGGGCTTGATAAGATAGAAGCGATAAAAAATGGCTGA", "aa": "MKKLSKRMQAVTKLIDKNKLYPIQEAFELIKKTAITKFVSSVDIAVSLNLDTTKAEQQLRGAIAFPFSIGKSIRILAITDDEKKASEAGADFVGGLDKIEAIKNG", "aa_hexdigest": "a656919bcd0b3a095363b3c59fd9c835", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplA" ], "truncated": "3-prime", "pseudogene": { "start": 109675, "stop": 110295, "observations": { "mutation": [ 109990 ], "stop": [ 109990 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47328", "query_cov": 1.0, "subject_cov": 0.4646017699115044, "identity": 1.0, "score": 197.0, "evalue": 4.55e-62, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MKKLSKRMQAVTKLIDKNKLYPIQEAFELIKKTAITKFVSSVDIAVSLNLDTTKAEQQLRGAIAFPFSIGKSIRILAITDDEKKASEAGADFVGGLDKIEAIKNGWLDFDLIITSPKFMGALGKLGKLLGTRGLMPNPKTETVTDDVVSAIKAYKKGKKEYRTDSFGNIHLSLGKTDTKTEHLVANAMALIDLIKSKRPSTVKGTYIKNIALTTTMGPSLKVKLPD" }, "paralog": false, "identity": 0.9951456310679612, "score": 393.0, "evalue": 5.55e-142, "description": "Internal stop codon at 109990. Nonsense mutation around 109990.", "psc": { "uniref90_id": "UniRef90_P47328", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_P47328", "cog_id": "COG0081", "cog_category": "J", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47328", "UniRef:UniRef50_P47328", "COG:COG0081", "COG:J", "KEGG:K02863", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P47328", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47328" ], "product": "Large ribosomal subunit protein uL1" } }, "id": "KKNAAMEMLJ_234", "locus": "GCA000027325_00195" }, { "type": "cds", "sequence": "contig_1", "start": 110029, "stop": 110355, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "nt": "ATGGGAGCATTAGGTAAACTAGGAAAACTATTAGGAACCAGGGGATTGATGCCAAACCCAAAAACTGAAACAGTTACTGATGATGTAGTTAGTGCTATTAAAGCTTATAAAAAGGGTAAGAAAGAATATCGAACTGATTCATTTGGCAACATCCACCTCTCTTTAGGTAAAACAGATACCAAAACTGAGCACTTGGTTGCTAATGCCATGGCTTTAATAGATTTAATTAAGTCTAAACGTCCTAGCACAGTCAAAGGTACTTACATTAAAAATATTGCTTTGACAACAACAATGGGACCAAGTTTAAAAGTAAAGCTACCTGATTAA", "aa": "MGALGKLGKLLGTRGLMPNPKTETVTDDVVSAIKAYKKGKKEYRTDSFGNIHLSLGKTDTKTEHLVANAMALIDLIKSKRPSTVKGTYIKNIALTTTMGPSLKVKLPD", "aa_hexdigest": "4256e6580f77ad810c76182ec7135e66", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2P6FDS1", "query_cov": 0.9629629629629629, "subject_cov": 0.9629629629629629, "identity": 0.5870000000000001, "score": 117.0, "evalue": 5.77e-32, "valid": false, "product": "Ribosomal protein", "uniref50_id": "UniRef50_A0A2P6FDS1", "kegg_orthology_id": "K02863" }, "pscc": { "uniref50_id": "UniRef50_A0A2P6FDS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "product": "Ribosomal protein" }, "genes": [], "id": "KKNAAMEMLJ_235", "locus": "GCA000027325_00196" }, { "type": "cds", "sequence": "contig_1", "start": 110355, "stop": 110924, "strand": "+", "frame": 3, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "GO:0005737", "GO:0006412", "RefSeq:WP_009885642.1", "SO:0001217", "UniParc:UPI0000132923", "UniRef:UniRef100_P47329", "UniRef:UniRef50_P47329", "UniRef:UniRef90_P47329" ], "nt": "ATGCCCACCTATAAACTAATTGTTGGTTTAGGTAACTTAGGTAAAAAGTATGAGAAAACTCGCCATAATGCTGGTTTTATGGTGTTAGATAGACTAGCTAGTTTATTCCACTTAAACTTTGATAAAACCAACAAGTTAGGTGATTATCTTTTTATTAAAGAAAAAGCAGCAATCTTAGCAAAACCTGCTACCTTTATGAATAATAGCGGTCTTTTTGTGAAATGGTTACAAGATCACTTTCAAATTCCGCTTGCAAACATAATGATAGTCCATGATGAAATAGCGTTTGATTTGGGAGTAATTAGGCTTAAAATGCAAGGGAGTGCTAACAATCATAATGGCATAAAATCAGTAATTAGACATTTAGATACTGAACAGTTCAATCGTTTACGCTTTGGGATTAAATCACAAAATACGAGTAACATATTGCATGAACAGGTAATGAGTGAATTCCAGAATAGTGAACTGACTAAACTGGAAGTTGCGATTACAAAGTCTGTTGAACTGTTGAAGCGTTATATTGAAGGAGAAGAGTTACAAAGGTTAATGGAATATTATCATCATGGCTAG", "aa": "MPTYKLIVGLGNLGKKYEKTRHNAGFMVLDRLASLFHLNFDKTNKLGDYLFIKEKAAILAKPATFMNNSGLFVKWLQDHFQIPLANIMIVHDEIAFDLGVIRLKMQGSANNHNGIKSVIRHLDTEQFNRLRFGIKSQNTSNILHEQVMSEFQNSELTKLEVAITKSVELLKRYIEGEELQRLMEYYHHG", "aa_hexdigest": "9408931b37249c9e048343d2b12aa2c3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000132923", "ncbi_nrp_id": "WP_009885642.1", "uniref100_id": "UniRef100_P47329", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000132923", "RefSeq:WP_009885642.1", "UniRef:UniRef100_P47329" ] }, "ips": { "uniref100_id": "UniRef100_P47329", "uniref90_id": "UniRef90_P47329" }, "psc": { "uniref90_id": "UniRef90_P47329", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_P47329", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045", "GO:0005737", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P47329", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47329" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "KKNAAMEMLJ_236", "locus": "GCA000027325_00197" }, { "type": "cds", "sequence": "contig_1", "start": 110917, "stop": 111129, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGTGAAAAACAATATATAGCAGGGGTTTCAGGTGGATCTGATTCAATGCTAATGCTTAAACTTTACCAAAAGAAGATTGCTTGTGTTGTTCATGTCAATTACAACACCAGATCAACCTCATTACGTGATCAAAAGTTAGTAGAACAATATTGTCAAAAATTAAATATTCCACTGGTTGTTCACACTGTTGATCCTGATCTAGTTTGA", "aa": "MASEKQYIAGVSGGSDSMLMLKLYQKKIACVVHVNYNTRSTSLRDQKLVEQYCQKLNIPLVVHTVDPDLV", "aa_hexdigest": "9bcf35473bb65cd8ff84750fd09c6bd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7869.1032000000005, "isoelectric_point": 8.640069007873539 }, "id": "KKNAAMEMLJ_237", "locus": "GCA000027325_00198" }, { "type": "cds", "sequence": "contig_1", "start": 111256, "stop": 111354, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTAGATGCAAAAAAACGTGCTGTTTACTATGGGATTAAAACCAGGTGTGAATTGTATGGTTTGAAAATCTACCGTCCCCTAATGAAATACTGA", "aa": "MQLDAKKRAVYYGIKTRCELYGLKIYRPLMKY", "aa_hexdigest": "349c372203ed58aa655e24dde8590348", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3913.719500000002, "isoelectric_point": 9.843049049377441 }, "id": "KKNAAMEMLJ_238", "locus": "GCA000027325_00199" }, { "type": "cds", "sequence": "contig_1", "start": 111445, "stop": 111789, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGTTAGAGATAGAAAAATGGTCTAAAATCGAAAAAGAACAGTTTTATATTGCTATCTGTGCAATGAACAAAACAATTGCTCAAAAACTGTTTGTATTAATGAAAAAAGCTAAAAAATGGTTATTACAACCTGATGTTAGGGAATTGAAACGGTTTTCAATTATTGATCAAAAACAGTTAATTTATAGCTATCTTATTTATCACAAGATTAATGTTAATGGTGAGAAAATTGATGCTATCCTTGATTTTATCCAACCTAGTCAACAAAAACAATACCGCTTGCAAAACGATATCTTTTTGATGGTGAAAAACCAGTGTTTAGCACTTTTATACAAAAGCTAA", "aa": "MRLEIEKWSKIEKEQFYIAICAMNKTIAQKLFVLMKKAKKWLLQPDVRELKRFSIIDQKQLIYSYLIYHKINVNGEKIDAILDFIQPSQQKQYRLQNDIFLMVKNQCLALLYKS", "aa_hexdigest": "e11f65335f95187cde31ac200bc29b48", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13758.283499999985, "isoelectric_point": 9.611091804504394 }, "id": "KKNAAMEMLJ_239", "locus": "GCA000027325_00200" }, { "type": "cds", "sequence": "contig_1", "start": 111790, "stop": 112725, "strand": "+", "frame": 1, "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "db_xrefs": [ "COG:COG1493", "COG:T", "EC:2.7.11.-", "EC:2.7.4.-", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0005829", "GO:0006109", "KEGG:K06023", "RefSeq:WP_010869325.1", "SO:0001217", "UniParc:UPI000012CB51", "UniRef:UniRef100_P47331", "UniRef:UniRef50_P75548", "UniRef:UniRef90_P47331" ], "nt": "ATGAAACATTTAACTGTCAAAGCATTAGTTTTGCAATTTAATGATTGTATCCAACTCATTGATGGTAAAAACAACATAGATAATGTGATTACTATCCCTGGGTTAAAAAGGAGTGTTTTTGAACTATTAGGACTATTTTGTAAGCCAATTGGTTCAGTTGCTATTTTAGGTAAACGTGAATTTATTTTTTTAAATCAAAAGCCAGTTGAGCAACAGAAAAAGATTATTGCAAACCTCTTGAAACTCAAACCACCTGCAGTTATTCTAACCAAGTCATTTCTTGATTGTGGTGTTTTGTTAGCTGTTAATCAAACTTATCAAGTTCCTATTTTAAAAACTAACTTGTTTTCAACTGAACTCTCTTTTACTGTTGAAACCTATATTAACGAGCAATTTGCTACTGTTCAAAAGTTACATGGGGTTTTACTTGAAATCTTTGGTGTTGGGGTATTTTTAGAAGGAAAGAGTGGGATTGGTAAATCTGAAAGTGCTTTAGATTTAATTAATAAAAACCACCTTCTAATAGGTGATGATGCTATTGAGATCTACAGATTAGGCAATAGGTTATTTGGTAGAGCACAAGCACTGGCAAAAGGCTTTATGGAGATTAGAGGTCTTGGCATCATTAATATTGAACGTGCTTATGGGTTACAGATTACAAAAGAACAAACTGAAATCCAACTAGCAATTAGTTTGTTGAGTTTAGAGGAGAAAAACAACGCTAGTTTTGAGCGCTTAGGCAGTGATTTAAAACTAAAAAATCTGCTTGGGGTTAAGATTAGTTACTATCAGATCCCTATCTCTTCAGGTAGAAAAACAAGCGAAATTATTGAAAGTGCAGTAATCGATTTTAAACTTAAGAAAAGTGGTTACAATTCAGCAAACGAGTTTATCTTAAAGCAACGTGCCATGTTAGAAGAACAAACTGATGAATAG", "aa": "MKHLTVKALVLQFNDCIQLIDGKNNIDNVITIPGLKRSVFELLGLFCKPIGSVAILGKREFIFLNQKPVEQQKKIIANLLKLKPPAVILTKSFLDCGVLLAVNQTYQVPILKTNLFSTELSFTVETYINEQFATVQKLHGVLLEIFGVGVFLEGKSGIGKSESALDLINKNHLLIGDDAIEIYRLGNRLFGRAQALAKGFMEIRGLGIINIERAYGLQITKEQTEIQLAISLLSLEEKNNASFERLGSDLKLKNLLGVKISYYQIPISSGRKTSEIIESAVIDFKLKKSGYNSANEFILKQRAMLEEQTDE", "aa_hexdigest": "af2ef0197b7a3fac3d8835a6976302ae", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012CB51", "ncbi_nrp_id": "WP_010869325.1", "uniref100_id": "UniRef100_P47331", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012CB51", "RefSeq:WP_010869325.1", "UniRef:UniRef100_P47331" ] }, "ips": { "uniref100_id": "UniRef100_P47331", "uniref90_id": "UniRef90_P47331" }, "psc": { "uniref90_id": "UniRef90_P47331", "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "ec_ids": [ "2.7.11.-", "2.7.4.-" ], "uniref50_id": "UniRef50_P75548", "cog_id": "COG1493", "cog_category": "T", "kegg_orthology_id": "K06023", "go_ids": [ "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0005829", "GO:0006109" ] }, "pscc": { "uniref50_id": "UniRef50_P75548", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75548" ], "product": "HPr kinase/phosphorylase" }, "genes": [ "hprK" ], "id": "KKNAAMEMLJ_240", "locus": "GCA000027325_00201" }, { "type": "cds", "sequence": "contig_1", "start": 112820, "stop": 113626, "strand": "+", "frame": 2, "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "db_xrefs": [ "COG:COG0682", "COG:M", "EC:2.5.1.145", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158", "SO:0001217", "UniRef:UniRef50_P47332", "UniRef:UniRef90_P47332" ], "nt": "ATGTTTGTCTTTAAACTGATTTATTGTTACAAATTACAAGACAACAGTTTTTATTTTTTTATCTTTATTGCTGTTTTAACGATGGTTTTAGGCGCTCGCCTCTGGTCATTTGTAATTGGTGATTCCAATTTTGCTAACAACAATTTCTTTGATTTTCGTAACGGTGGATTGGCCATTCAGGGTGGGATTTTGTTAACCAGTATTGTCGGAGTAATCTATTTCAACTTCTTTTTAAATAGTAAGACCAATAAAACCAAAACGATTGCTGAATTACTGAATAATAAGAATGAAATAAAAGCTGTTTATGTTGAAAGAAATATCTCTGTTCTAGTGATGTTAGATCTGATTGCTCCTTGTGTTTTAATTGGTCAAGCAATTGGCAGATGGGGTAATTTTTTCAACCAAGAAGTTTATGGGTTTGCTTTAGCTGGAACAATGAATGATCCCCAAGCATTGGCTAATACCCAGTGGGGATTTTTAAAGATCTTAATGCCTAAGGTTTGGGATGGGATGTGGATTGATGGTCAGTTTCGCATTCCGCTCTTTTTAATTGAGTCATTTTTTAACACTATTTTCTTTGTGTTAATTTACTTTGTAATGGATTTTATTAGGGGAGTTAAAAGTGGCACAATTGGTTTTAGTTATTTTCTTGCTACTGGAATCATTCGTTTAATCTTGGAAAACTTTAGAGACCAAACCTTTTATTTTCAAACTTCAATAACCACTAGTATTTTGTTTATTGTCGTTGGTATTTTAGGAATTTTTTATTGCCAGTTTATCCATGTCAAATTAAGAAATTACTTCTGA", "aa": "MFVFKLIYCYKLQDNSFYFFIFIAVLTMVLGARLWSFVIGDSNFANNNFFDFRNGGLAIQGGILLTSIVGVIYFNFFLNSKTNKTKTIAELLNNKNEIKAVYVERNISVLVMLDLIAPCVLIGQAIGRWGNFFNQEVYGFALAGTMNDPQALANTQWGFLKILMPKVWDGMWIDGQFRIPLFLIESFFNTIFFVLIYFVMDFIRGVKSGTIGFSYFLATGIIRLILENFRDQTFYFQTSITTSILFIVVGILGIFYCQFIHVKLRNYF", "aa_hexdigest": "41fee8aef0cce8b48f00e2414e72cf1a", "start_type": "ATG", "rbs_motif": null, "genes": [ "lgt" ], "truncated": "both", "pseudogene": { "start": 112718, "stop": 113866, "observations": { "mutation": [ 112733, 113624 ], "stop": [ 112733, 113624 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47332", "query_cov": 1.0, "subject_cov": 0.7015706806282722, "identity": 1.0, "score": 529.0, "evalue": 3.55e-188, "gene_start": 1, "gene_end": 268, "reference_start": 35, "reference_end": 302, "reference_sequence": "MNRPSWSTAFNIGGGFPIQWYGIIVSIGIIFAILMFVFKLIYCYKLQDNSFYFFIFIAVLTMVLGARLWSFVIGDSNFANNNFFDFRNGGLAIQGGILLTSIVGVIYFNFFLNSKTNKTKTIAELLNNKNEIKAVYVERNISVLVMLDLIAPCVLIGQAIGRWGNFFNQEVYGFALAGTMNDPQALANTQWGFLKILMPKVWDGMWIDGQFRIPLFLIESFFNTIFFVLIYFVMDFIRGVKSGTIGFSYFLATGIIRLILENFRDQTFYFQTSITTSILFIVVGILGIFYCQFIHVKLRNYFWTYFFLYAFYKVAAFFTTLFLNNRKQMAQQKFAFYEKSLPNKKRSFFEMKYYNDVTTPKIYRLTDQEMKLFDKLEAVTTS" }, "paralog": false, "identity": 0.9947643979057592, "score": 764.0, "evalue": 2.23e-283, "description": "Internal stop codon at 112733, 113624. Nonsense mutation around 112733, 113624.", "psc": { "uniref90_id": "UniRef90_P47332", "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "ec_ids": [ "2.5.1.145" ], "uniref50_id": "UniRef50_P47332", "cog_id": "COG0682", "cog_category": "M", "go_ids": [ "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47332", "EC:2.5.1.145", "UniRef:UniRef50_P47332", "COG:COG0682", "COG:M", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ] }, "pscc": { "uniref50_id": "UniRef50_P47332", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47332" ], "product": "Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase" } }, "id": "KKNAAMEMLJ_241", "locus": "GCA000027325_00202" }, { "type": "cds", "sequence": "contig_1", "start": 113702, "stop": 113866, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACAACAGAAGTTTGCTTTTTATGAAAAATCACTTCCCAATAAGAAGCGTTCTTTTTTTGAAATGAAGTATTACAATGATGTAACAACACCCAAAATTTATCGTTTAACTGATCAGGAAATGAAGTTATTTGATAAATTAGAGGCAGTTACAACCAGCTAG", "aa": "MAQQKFAFYEKSLPNKKRSFFEMKYYNDVTTPKIYRLTDQEMKLFDKLEAVTTS", "aa_hexdigest": "442510d988d14817b06dcfebc104c88e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6550.491400000001, "isoelectric_point": 9.301321220397949 }, "id": "KKNAAMEMLJ_242", "locus": "GCA000027325_00203" }, { "type": "cds", "sequence": "contig_1", "start": 113895, "stop": 114314, "strand": "+", "frame": 3, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_010869326.1", "SO:0001217", "UniParc:UPI0000134C58", "UniRef:UniRef100_P47333", "UniRef:UniRef50_P92532", "UniRef:UniRef90_P47333" ], "nt": "ATGGCTACAATAGCGCAATTAATTAGAAAACCACGCCAAAAAAAGAAGGTTAAATCAAAGTCACCTGCACTCCATTATAACCTCAACCTTTTAAACAAAAAAACTACCAATGTTTACTCACCACTAAAGCGTGGTGTTTGCACCAGGGTTGGCACCATGACCCCCAGAAAACCTAATTCTGCACTAAGAAAGTATGCTAAGGTTAGACTTACAAATGGCTTTGAAGTACTTGCTTATATCCCAGGAGAAGGTCATAACCTACAAGAACACAGTGTTACTTTATTAAGGGGGGGTAGAGTAAAAGATCTCCCTGGAGTTAGATACCATATTGTTCGTGGTACTTTAGATACAGTTGGTGTTGACAAAAGAAGACAACAACGTTCTGCATATGGCGCTAAAAAACCAAAACCAAAATCTTAA", "aa": "MATIAQLIRKPRQKKKVKSKSPALHYNLNLLNKKTTNVYSPLKRGVCTRVGTMTPRKPNSALRKYAKVRLTNGFEVLAYIPGEGHNLQEHSVTLLRGGRVKDLPGVRYHIVRGTLDTVGVDKRRQQRSAYGAKKPKPKS", "aa_hexdigest": "15b51a4caebf9e021f3072bb40f6189b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134C58", "ncbi_nrp_id": "WP_010869326.1", "uniref100_id": "UniRef100_P47333", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134C58", "RefSeq:WP_010869326.1", "UniRef:UniRef100_P47333" ] }, "ips": { "uniref100_id": "UniRef100_P47333", "uniref90_id": "UniRef90_P47333" }, "psc": { "uniref90_id": "UniRef90_P47333", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "genes": [ "rpsL" ], "id": "KKNAAMEMLJ_243", "locus": "GCA000027325_00204" }, { "type": "cds", "sequence": "contig_1", "start": 114331, "stop": 114798, "strand": "+", "frame": 1, "gene": "rpsG", "product": "30S ribosomal protein S7", "db_xrefs": [ "COG:COG0049", "COG:J", "GO:0000028", "GO:0000049", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "KEGG:K02992", "RefSeq:WP_009885645.1", "SO:0001217", "UniParc:UPI0000135107", "UniRef:UniRef100_P47334", "UniRef:UniRef50_P21469", "UniRef:UniRef90_P47334" ], "nt": "ATGAGAAAAAATCGTGCTTTAAAAAGAACTGTTTTACCCGATCCTGTTTTTAACAACACACTGGTTACAAGGATTATTAATGTCATCATGAAAGATGGCAAGAAGGGTTTAGCACAACGCATCTTGTATGGTGCTTTTGAGATCATTGAAAAACGCACCAACCAACAACCTTTAACTGTCTTTGAAAAAGCAGTTGATAATGTTATGCCCCGCTTAGAGTTAAAAGTGAGAAGAATTGCTGGTTCTAACTACCAAGTACCAACTGAAGTTCCCCCTGACAGAAGGATTGCTTTAGCACTAAGATGGATTGTGATCTTTGCTAACAAAAGAAATGAAAAAACAATGCTTGAACGTGTTGCTAATGAAATTATTGATGCTTTTAATAACACGGGTGCTAGTGTTAAAAAGAAGGATGATACTCACAAGATGGCAGAAGCTAACAAAGCCTTTGCCCACATGCGTTGGTAG", "aa": "MRKNRALKRTVLPDPVFNNTLVTRIINVIMKDGKKGLAQRILYGAFEIIEKRTNQQPLTVFEKAVDNVMPRLELKVRRIAGSNYQVPTEVPPDRRIALALRWIVIFANKRNEKTMLERVANEIIDAFNNTGASVKKKDDTHKMAEANKAFAHMRW", "aa_hexdigest": "c83a70d858afbe8347c2153f5149a144", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135107", "ncbi_nrp_id": "WP_009885645.1", "uniref100_id": "UniRef100_P47334", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135107", "RefSeq:WP_009885645.1", "UniRef:UniRef100_P47334" ] }, "ips": { "uniref100_id": "UniRef100_P47334", "uniref90_id": "UniRef90_P47334" }, "psc": { "uniref90_id": "UniRef90_P47334", "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_P21469", "cog_id": "COG0049", "cog_category": "J", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0000028", "GO:0000049", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P21469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P21469" ], "product": "Small ribosomal subunit protein uS7" }, "genes": [ "rpsG" ], "id": "KKNAAMEMLJ_244", "locus": "GCA000027325_00205" }, { "type": "cds", "sequence": "contig_1", "start": 114808, "stop": 115023, "strand": "+", "frame": 1, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "nt": "ATGTCAAGAACAGTTGATTTAAAAAACTTCCGTAACTTTGGCATTATGGCCCATATTGATGCTGGGAAAACCACCACATCAGAACGTATTTTGTTCCATTCAGGTAGAATTCACAAGATTGGTGAAACCCATGATGGTGAATCAGTGATGGACTGGATGGAACAAGAAAAAGAAAGGGGTATTACTATCACCTCTGCAGCCACTTCAGTGAGCTGA", "aa": "MSRTVDLKNFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVS", "aa_hexdigest": "f943b1f57d508f2d22013d85c521b884", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 0.971830985915493, "subject_cov": 0.9324324324324325, "identity": 0.7390000000000001, "score": 109.0, "evalue": 3.86e-30, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" }, "genes": [ "lepA" ], "id": "KKNAAMEMLJ_245", "locus": "GCA000027325_00206" }, { "type": "cds", "sequence": "contig_1", "start": 115042, "stop": 115167, "strand": "+", "frame": 1, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E2F5E1A", "UniRef:UniRef90_UPI000E2F5E1A" ], "nt": "TTGATTGACACTCCTGGCCATGTTGACTTTACAGTTGAAGTGGAGCGTAGCTTAAGGGTTTTGGATGGAGCAATTGCGGTATTGGATGCTCAAATGGGAGTAGAACCACAAACTGAAACAGTATGA", "aa": "MIDTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETV", "aa_hexdigest": "5fdc6e462382a49e41aa5e7c6538dacc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 115036, "stop": 115254, "observations": { "mutation": [ 115165 ], "stop": [ 115165 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000E2F5E1A", "query_cov": 1.0, "subject_cov": 0.5256410256410257, "identity": 0.927, "score": 79.0, "evalue": 1.92e-18, "gene_start": 1, "gene_end": 41, "reference_start": 8, "reference_end": 48, "reference_sequence": "WRGHRVNIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKIGADFLYSVG" }, "paralog": false, "identity": 0.8055555555555556, "score": 117.0, "evalue": 1.76e-36, "description": "Internal stop codon at 115165. Nonsense mutation around 115165.", "psc": { "uniref90_id": "UniRef90_UPI000E2F5E1A", "product": "GTP-binding protein", "uniref50_id": "UniRef50_UPI000E2F5E1A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000E2F5E1A", "UniRef:UniRef50_UPI000E2F5E1A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000E2F5E1A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E2F5E1A" ], "product": "GTP-binding protein" } }, "id": "KKNAAMEMLJ_246", "locus": "GCA000027325_00207" }, { "type": "cds", "sequence": "contig_1", "start": 115216, "stop": 116874, "strand": "+", "frame": 1, "gene": "fusA", "product": "elongation factor G", "db_xrefs": [ "COG:COG0480", "COG:J", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790", "KEGG:K02355", "SO:0001217", "UniRef:UniRef50_Q5SHN5", "UniRef:UniRef90_P75544" ], "nt": "ATGGATAAAACCGGTGCTAACTTTGAGCGCTCTGTTTTATCAATTCAACAACGCTTGGGAGTGAAAGCTGTTCCTATTCAATTTCCCATAGGTGCTGAAAATGATTTCAATGGCATCATTGATATCATCACTAAAAAAGCTTATTTTTTTGATGGTAATAAAGAGGAAAATGCTATTGAAAAACCAATTCCTGAACAGTATGTTGATCAAGTTGAAAAACTTTACAACAACTTAGTTGAAGAAGTTGCTAGTTTAGATGATCAACTCATGGCTGATTATCTAGATGGTAAACCAATTGAAATTGATGCAATTAAAAATGCAATTAGAAACGGGGTAATTCACTGTAAGTTTTTCCCGGTATTGTGTGGTTCAGCATTTAAAAACAAGGGAATTAAACTCTTACTTGATGCAGTGGTTGATTTTCTCCCTTCACCTGTTGATGTCCCACCTGCTAAAGCAATTGATGCAAACAACAAAGAGATATCTATTAAAGCTAGTGATGATGCTAACTTTATTGGCTTAGCATTTAAAGTTGCTACTGATCCTTTTGTTGGTAGATTAACTTTTATTAGGGTTTATGCAGGAGTTTTAAAATCTGGTTCTTATGTTAAGAATGTTAGAAAAAACAAAAAGGAAAGGGTATCACGTTTAGTGAAAATGCACGCACAAAATCGCAATGAAATTGATGAAATTAGAGCAGGGGATATCTGTGCAGTAATTGGCTTGAAAGATACTACTACTGGAGAAACTTTAACTGATGATAAGCTTGATGTGCAACTAGAAGCAATGCAATTTGCTGAACCAGTGATCTCTTTAGCAGTAGAACCTAAAACTAAAGCAGATCAGGAAAAGATGTCAATTGCTTTATCAAAACTAGCAGAAGAAGATCCTACTTTTAAAACCTTTAGTGATCCTGAAACAGGGCAAACTATTATTGCTGGAATGGGTGAGTTACACCTTGATATCTTAGTTGATAGGATGAAACGTGAATTTAAGGTAGAAGTTAACATTGGTGCACCTCAAGTTAGCTTTCGTGAAACCTTTAAATCAACTAGTGAAGTTGAGGGTAAATACATCAAACAATCAGGTGGTAGAGGTCAATATGGACATGTTAAAATCCGTTTTGAACCTAATAAAGATAAGGGCTTTGAATTTGTTGATAAGATTGTGGGCGGAAGGATTCCAAGGGAATATATTAAACCAGTTCAAACTGGTCTTGAAAATGCAATGAATTCAGGTCCTTTAGCAGGTTACCCAATGATTGATATTAAAGCTACCTTATTTGATGGTTCTTTCCATGAAGTTGACTCAAGTGAAATGGCTTTTAAAATTGCTGCATCCTTAGCTTTAAAAGAAGCAGGTAAACAATGTAACCCAGTTTTACTTGAACCTATTATGGCAATAGAAGTTACTGTACCTGAACAGTACTTTGGGGATACAATGGGTGATATCAGTTCAAGAAGAGGGATCATTGAAGGTACTGAACAACGTGATAATGTTCAACTAATAAAAGCAAAAGTACCTTTAAAAGAGATGTTTGGTTATGCCACTGATTTACGCTCTTTTTCCCAAGGTAGGGGTAATTATGTAATGCAATTTAGCCATTATGCTGAAACTCCTAAAAGCGTTGTTAATGAGATAATTGCTAATAAAAAATAG", "aa": "MDKTGANFERSVLSIQQRLGVKAVPIQFPIGAENDFNGIIDIITKKAYFFDGNKEENAIEKPIPEQYVDQVEKLYNNLVEEVASLDDQLMADYLDGKPIEIDAIKNAIRNGVIHCKFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPPAKAIDANNKEISIKASDDANFIGLAFKVATDPFVGRLTFIRVYAGVLKSGSYVKNVRKNKKERVSRLVKMHAQNRNEIDEIRAGDICAVIGLKDTTTGETLTDDKLDVQLEAMQFAEPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFSDPETGQTIIAGMGELHLDILVDRMKREFKVEVNIGAPQVSFRETFKSTSEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQTGLENAMNSGPLAGYPMIDIKATLFDGSFHEVDSSEMAFKIAASLALKEAGKQCNPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGIIEGTEQRDNVQLIKAKVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIANKK", "aa_hexdigest": "be2b51dd2f0eb0c723dcff08f105a76c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75544", "query_cov": 1.0, "subject_cov": 0.8023255813953488, "identity": 1.0, "score": 1077.0, "evalue": 0.0, "valid": true, "gene": "fusA", "product": "elongation factor G", "uniref50_id": "UniRef50_Q5SHN5", "cog_id": "COG0480", "cog_category": "J", "kegg_orthology_id": "K02355", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHN5" ], "product": "Elongation factor G" }, "genes": [ "fusA" ], "id": "KKNAAMEMLJ_247", "locus": "GCA000027325_00208" }, { "type": "cds", "sequence": "contig_1", "start": 116926, "stop": 117396, "strand": "+", "frame": 1, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0022627", "GO:0070181", "GO:1990904", "KEGG:K02990", "SO:0001217", "UniRef:UniRef50_P47336", "UniRef:UniRef90_P47336" ], "nt": "ATGCACTACAATATCATTCTTTTAGTTGATGGTACGCTTAGTTTAGAACAAGCTAACCAAGTTGAACAAAAACACCAAAAATTGCTTGAAAAGGCAACTGAATTTAAAAGTGAATACTTAGGTTTAAAAGAGTTGGCTTACCCCATTAAAAAGCAACTTTCTGCTCACTATTACAGATGGAGTTTTCATGGTGAAAGCAATTGTACTAAGGAGTTTAAAAGAGCTGCTAACATCAATAAGCAGATAATAAGAGAGTTAATTATTAACAGAGAAAAAGACTATGGTTATTTAGGTTCAGTTAACCCTAAAAAACAACAACTGTCTTTGCAGAAGCTAACCAAGTATAATGAGATTATTGCTAGTGAAAATAATCCTGATAACCCAGATGCGCCTGTCACTTCTGGTCTAGCTTCTGTTAAACCACGGCTATCAAGAGTTGAAAAACAAAAGGAACGTGAACTTGAAAAGTGA", "aa": "MHYNIILLVDGTLSLEQANQVEQKHQKLLEKATEFKSEYLGLKELAYPIKKQLSAHYYRWSFHGESNCTKEFKRAANINKQIIRELIINREKDYGYLGSVNPKKQQLSLQKLTKYNEIIASENNPDNPDAPVTSGLASVKPRLSRVEKQKERELEK", "aa_hexdigest": "97462fe86103323364dc6241cad6a575", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpsF" ], "truncated": "3-prime", "pseudogene": { "start": 116926, "stop": 117552, "observations": { "mutation": [ 117394 ], "stop": [ 117394 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47336", "query_cov": 1.0, "subject_cov": 0.75, "identity": 1.0, "score": 301.0, "evalue": 8.18e-103, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MHYNIILLVDGTLSLEQANQVEQKHQKLLEKATEFKSEYLGLKELAYPIKKQLSAHYYRWSFHGESNCTKEFKRAANINKQIIRELIINREKDYGYLGSVNPKKQQLSLQKLTKYNEIIASENNPDNPDAPVTSGLASVKPRLSRVEKQKERELEKWTVVHQSGNFDTVQINPYRPRIKRFLQNNQQTSQANNNQPRFQNQFKKGAKP" }, "paralog": false, "identity": 0.9951923076923077, "score": 416.0, "evalue": 2.11e-150, "description": "Internal stop codon at 117394. Nonsense mutation around 117394.", "psc": { "uniref90_id": "UniRef90_P47336", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_P47336", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0022627", "GO:0070181", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47336", "UniRef:UniRef50_P47336", "COG:COG0360", "COG:J", "KEGG:K02990", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0022627", "GO:0070181", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47336" ], "product": "Small ribosomal subunit protein bS6" } }, "id": "KKNAAMEMLJ_248", "locus": "GCA000027325_00209" }, { "type": "cds", "sequence": "contig_1", "start": 117745, "stop": 118035, "strand": "+", "frame": 1, "gene": "ssb", "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "COG:COG0629", "COG:L", "GO:0003697", "GO:0006260", "GO:0009295", "SO:0001217", "UniRef:UniRef50_P75542", "UniRef:UniRef90_P47337" ], "nt": "ATGCTTTTTGTAGAAGGAAGATTAACTACATATAAAATTACTAACAGTGAAAATAAAAACACCTATGCTTTACAAGTAACTGCTGATAAGATCTTTCATCCTGATGAAAAAACTACCAATGAAGAACCTATTAAATCAACTGTAGTTGATTCACCCTTTATGAATCCCAAAGCAAGTGTTACAGAAGCTGAGTTTGAACAAGCATTCCCCCATCAAGATGAAACTGATTTTAACAACATTACCCCTATATTTGAAAATGATGTCCAACTAGAGGAGGAAAGTGATGATTAA", "aa": "MLFVEGRLTTYKITNSENKNTYALQVTADKIFHPDEKTTNEEPIKSTVVDSPFMNPKASVTEAEFEQAFPHQDETDFNNITPIFENDVQLEEESDD", "aa_hexdigest": "63c4e090fab5027f158933171c84a084", "start_type": "ATG", "rbs_motif": null, "genes": [ "ssb" ], "truncated": "5-prime", "pseudogene": { "start": 117553, "stop": 118035, "observations": { "mutation": [ 117700 ], "stop": [ 117700 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47337", "query_cov": 1.0, "subject_cov": 0.6, "identity": 1.0, "score": 190.0, "evalue": 2.11e-60, "gene_start": 1, "gene_end": 96, "reference_start": 65, "reference_end": 160, "reference_sequence": "MNRVFLFGKLSFTPNRLQTKNGTLGATFSMECLDSSGFNNAKSFIRVTAWGKVASFIVAQNPGVMLFVEGRLTTYKITNSENKNTYALQVTADKIFHPDEKTTNEEPIKSTVVDSPFMNPKASVTEAEFEQAFPHQDETDFNNITPIFENDVQLEEESDD" }, "paralog": false, "identity": 0.99375, "score": 319.0, "evalue": 8.96e-114, "description": "Internal stop codon at 117700. Nonsense mutation around 117700.", "psc": { "uniref90_id": "UniRef90_P47337", "gene": "ssb", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_P75542", "cog_id": "COG0629", "cog_category": "L", "go_ids": [ "GO:0009295", "GO:0003697", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47337", "UniRef:UniRef50_P75542", "COG:COG0629", "COG:L", "GO:0009295", "GO:0003697", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_P75542", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75542" ], "product": "Single-stranded DNA-binding protein" } }, "id": "KKNAAMEMLJ_249", "locus": "GCA000027325_00210" }, { "type": "cds", "sequence": "contig_1", "start": 118028, "stop": 118342, "strand": "+", "frame": 2, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181", "RefSeq:WP_009885648.1", "SO:0001217", "UniParc:UPI0000557C7D", "UniRef:UniRef100_P47338", "UniRef:UniRef50_P47338", "UniRef:UniRef90_P47338" ], "nt": "ATGATTAATAAAGAACAGGATTTAAACCAATTAGAAACCAACCAAGAACAGAGTGTTGAACAAAACCAAACTGATGAAAAGCGCAAGCCAAAACCAAACTTTAAAAGAGCAAAAAAATATTGTCGATTTTGCGCCATAGGTCAACTAAGGATTGATTTTATTGATGATTTGGAAGCAATCAAACGCTTTCTCAGTCCCTATGCAAAGATTAATCCTAGAAGAATTACAGGTAATTGCAACATGCACCAACGTCATGTAGCTAATGCTCTAAAACGAGCACGTTACCTAGCTTTAGTGCCATTTATTAAAGATTAA", "aa": "MINKEQDLNQLETNQEQSVEQNQTDEKRKPKPNFKRAKKYCRFCAIGQLRIDFIDDLEAIKRFLSPYAKINPRRITGNCNMHQRHVANALKRARYLALVPFIKD", "aa_hexdigest": "f3db5ce4b19d0a295cf2c340b55e09b3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557C7D", "ncbi_nrp_id": "WP_009885648.1", "uniref100_id": "UniRef100_P47338", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557C7D", "RefSeq:WP_009885648.1", "UniRef:UniRef100_P47338" ] }, "ips": { "uniref100_id": "UniRef100_P47338", "uniref90_id": "UniRef90_P47338" }, "psc": { "uniref90_id": "UniRef90_P47338", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_P47338", "cog_id": "COG0238", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47338" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "KKNAAMEMLJ_250", "locus": "GCA000027325_00211" }, { "type": "cds", "sequence": "contig_1", "start": 118345, "stop": 118797, "strand": "+", "frame": 1, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02939", "RefSeq:WP_009885649.1", "SO:0001217", "UniParc:UPI00001341F1", "UniRef:UniRef100_P47339", "UniRef:UniRef50_P75540", "UniRef:UniRef90_P47339" ], "nt": "ATGAAGATAATTTTGAAGCAAGATGTTGCTAAATTAGGCAAGCGGTTTGATGTTGTTGAAGTTAAAGATGGGTTTGCTATCCATTTTTTATTTCCCAAAAAACTAGCTGCACCTTTAACAAAGAAAGCAATTGCTAACCGTGATTTGTTTTTAAAACAACAACAAGAACAATACCAAAAAAATCGTGCCTTAGCTGAAAAATTGAAACTAGTAATTGAACAAACACCATTAACTTTTCAACTCAAACAACACGATGGCAAGCCATATGGTTCAATCATCACCAAACAAATAATTAATTTAGCAAAACAACAAAGACTTGATTTACAGCGCTTTATGTTTAAAGATAATGTGCGCTTACAGTTTGGTGAACACAAACTAATTTTGCACCTTTTTGAGGAGATAACTGCAACTTTAACTGTTATAGTGAACCCTGAAAATGGGACAACAAACTAG", "aa": "MKIILKQDVAKLGKRFDVVEVKDGFAIHFLFPKKLAAPLTKKAIANRDLFLKQQQEQYQKNRALAEKLKLVIEQTPLTFQLKQHDGKPYGSIITKQIINLAKQQRLDLQRFMFKDNVRLQFGEHKLILHLFEEITATLTVIVNPENGTTN", "aa_hexdigest": "bcfbc0281b4519f95d168d7380d55c03", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001341F1", "ncbi_nrp_id": "WP_009885649.1", "uniref100_id": "UniRef100_P47339", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341F1", "RefSeq:WP_009885649.1", "UniRef:UniRef100_P47339" ] }, "ips": { "uniref100_id": "UniRef100_P47339", "uniref90_id": "UniRef90_P47339" }, "psc": { "uniref90_id": "UniRef90_P47339", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_P75540", "cog_id": "COG0359", "cog_category": "J", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75540", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75540" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "KKNAAMEMLJ_251", "locus": "GCA000027325_00212" }, { "type": "cds", "sequence": "contig_1", "start": 118781, "stop": 119410, "strand": "+", "frame": 2, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P47340" ], "nt": "ATGGGACAACAAACTAGTTTTAAATATGCAAATGATAGCAACATTGAACGTGCTGAAAGACGTTTGATGCAAGCAGTTGCTCAAAACAGTGAGGGCATTGATCTAATTTTCAATAAACTTGAACCAATTGATTTTTTTGCAACCCCTTTCAAACTCATTTTTCAAACTGCAAAAGAAAACTACCAATTAAATAACCCTATTATTGGTTCTGGTTTACTAGAAGCGGTTAAGTTTAAACTTGATGCTAATGATCAATCCACTAAAAGTGAACTTGAAATTTTATTCACAAAGATCTTATTAATCCGTTTACCACCTAACCAAACAGAGATTAAAACACTGGTTGATGTTGTTAAAAAAGCTTCTATTTTTCGCAGGTTACAACAGTTTGCTAAGCGTGTTTACAACGAGGAATTTAAGTTAAAAGAAGATCGTTTTGAAGGCTATTTACAAGCTATTCAAGATGATTTTGTCAAGATTATCCACAGTGCTTTTAGTAACATCTTTGCTTTTAGCTATGATGAGATTGCCAATCAAGAGGAAGCATTAATTAAAAAGGTTCACCGTGGGGAATTGATCATCAGTGGACTTTCAAGTGGATTTTTAAAATTAGATCAACTTACATCAGGTTGA", "aa": "MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELEILFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSG", "aa_hexdigest": "9fc704a78e823d94adaf9bdad0485551", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "3-prime", "pseudogene": { "start": 118781, "stop": 119713, "observations": { "mutation": [ 119408 ], "stop": [ 119408 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47340", "query_cov": 1.0, "subject_cov": 0.4465811965811966, "identity": 1.0, "score": 400.0, "evalue": 5.36e-137, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELEILFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSGWKPGELIVIAARPGRGKTALLINFMASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS" }, "paralog": false, "identity": 0.9967741935483871, "score": 595.0, "evalue": 3.54e-216, "description": "Internal stop codon at 119408. Nonsense mutation around 119408.", "psc": { "uniref90_id": "UniRef90_P47340", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47340", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "KKNAAMEMLJ_252", "locus": "GCA000027325_00213" }, { "type": "cds", "sequence": "contig_1", "start": 119480, "stop": 120187, "strand": "+", "frame": 2, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P47340" ], "nt": "ATGGCTAGTGCAGCTAAACAAATTGATCCTAAAACTGATGTGGTCCTCTTCTTTAGTTTAGAGATGCGTAACCGGGAAATTTACCAAAGGCACTTAATGCATGAAAGTCAAACTAGTTACACACTAACCAACCGGCAAAGGATTAATAATGTCTTTGAAGAGTTAATGGAAGCATCTTCAAGGATCAAAAACTTACCTATTAAACTCTTTGATTACAGTAGTTTAACACTCCAAGAGATCAGAAACCAAATTACTGAAGTGAGTAAAACCAGTAATGTTAGGTTAGTAATTATTGACTATTTACAACTTGTTAATGCTTTAAAAAATAACTATGGTTTGACAAGACAACAAGAAGTGACAATGATCTCTCAATCACTTAAAGCATTCGCTAAGGAGTTTAATACCCCTATTATTGCTGCAGCTCAACTTTCTAGAAGGATTGAAGAAAGGAAAGATTCCAGACCAATTCTTTCTGATTTAAGAGAATCAGGTTCAATTGAACAGGATGCGGATATGGTTTTATTTATCCATAGAACTAATGATGATAAAAAAGAACAGGAAGAGGAGAACACAAACTTGTTTGAAGTGGAGCTTATCTTAGAAAAGAACAGAAATGGTCCCAATGGCAAAGTTAAACTAAACTTTCGCAGTGACACTTCTTCTTTTATTAGTCAATATTCCCCTAGTTTTGATGACCAATACAGTTAA", "aa": "MASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS", "aa_hexdigest": "b5651c07e719f73098f7488ccd5f6632", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 119180, "stop": 120187, "observations": { "mutation": [ 119408 ], "stop": [ 119408 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47340", "query_cov": 1.0, "subject_cov": 0.5021367521367521, "identity": 1.0, "score": 441.0, "evalue": 8.85e-153, "gene_start": 1, "gene_end": 235, "reference_start": 234, "reference_end": 468, "reference_sequence": "MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELEILFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSGWKPGELIVIAARPGRGKTALLINFMASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS" }, "paralog": false, "identity": 0.9970149253731343, "score": 644.0, "evalue": 4.72e-235, "description": "Internal stop codon at 119408. Nonsense mutation around 119408.", "psc": { "uniref90_id": "UniRef90_P47340", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47340", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "KKNAAMEMLJ_253", "locus": "GCA000027325_00214" }, { "type": "cds", "sequence": "contig_1", "start": 120191, "stop": 120538, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTGAAGTTTTACAAACTACCTTTAATTACTACAGCATTTAGTTTTGTATTTTTAACTGCTTGTTCAACACCCCAATCTTCCTTTTTTCTCCCTGCACAAACAACTATTAGTACTTTAAAAATTAATGGTATGGAAAACAAAACAGGTTATTTTCTTGAAACGCAGCGTTCTCGTGGTAGTTACAATCCCACCGCTTCACTTACTATGATTAAGTTAGGAGATGAAAAAGAGTTTAAAAACGTAGATACAACCAAGCAAGATGAAGTTTTGTTTGCCAACATTTATGGTGGAATCTCTTCATTGCTTAACTTCAGAATTATCCAACCGATGTTAACCTACTGA", "aa": "MKLKFYKLPLITTAFSFVFLTACSTPQSSFFLPAQTTISTLKINGMENKTGYFLETQRSRGSYNPTASLTMIKLGDEKEFKNVDTTKQDEVLFANIYGGISSLLNFRIIQPMLTY", "aa_hexdigest": "4239874428945bbd1f667f1e9f076b0c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13014.951299999982, "isoelectric_point": 9.300869941711426 }, "id": "KKNAAMEMLJ_254", "locus": "GCA000027325_00215" }, { "type": "cds", "sequence": "contig_1", "start": 120857, "stop": 120985, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGGTATTGAAAATATTGAAGTTGAGTTTAGCTTTGTTAAAACTGGTTTTAATGGAAATGAGATTAAGTTTGAAGATTATGTAACAGATTCTTCCCCCACAACATCTTTATTAAAGCAAGTTTGA", "aa": "MIGIENIEVEFSFVKTGFNGNEIKFEDYVTDSSPTTSLLKQV", "aa_hexdigest": "8ed62026fe40d4ea6cd97c5ff932fbec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4728.241900000001, "isoelectric_point": 4.250954246520997 }, "id": "KKNAAMEMLJ_255", "locus": "GCA000027325_00216" }, { "type": "cds", "sequence": "contig_1", "start": 121214, "stop": 121387, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACTAACTGATTTGCAAAAGAAACAAGATCAAGTGAATCGGTTTGTTGGTTTTATTGGTCAAAACCACTTTACTTTAAGTGCTAACAGCATTAATGAAAAGCAGTTTAACGATGCTAGTACTGCTGATTTTTTTAAAGCAATCTTTCAAAAAGTGAGTATCAATGAATAA", "aa": "MLLTDLQKKQDQVNRFVGFIGQNHFTLSANSINEKQFNDASTADFFKAIFQKVSINE", "aa_hexdigest": "b143ce62319daa644f13bee0d843ce30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6538.271699999999, "isoelectric_point": 6.541849708557129 }, "id": "KKNAAMEMLJ_256", "locus": "GCA000027325_00217" }, { "type": "cds", "sequence": "contig_1", "start": 121571, "stop": 123202, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG096", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47342", "UniRef:UniRef90_P47342" ], "nt": "TTGAAAACACAAAAAAATATCTTTAAGGTCAAGGCCTTACTACTTTCACTTTTTTCTGCTGCTGTTACCATCACTATTTTTGCTTTACCTATCTTTGCAAACAATGGTTCTAAAACTGATTTAGGTTTGCTTTCTAAAAATAGTGCTGATTTTTTAGGTAGTTCAAAAAGAAGTCTTGCTGGCTTTGATACTCCTTTTAGTCCAGATAACCTCCAGTATTTAGAAAAAGAAACTGATTATGATCAGAACTTTAAAAGTTTTACTGAAAAGTTTAAAGATGAAAAAATAACTAACAACCAACTTGGTATTGTTGATATCTATAACTTATTCAGTGGTTTTCACAAGAGTGTCAAAAGCACAGTTGACTTAATGAACCAACTGCAAAAGCAAGTTGAAGCTGCTAATGCTATCTTCCCAGTTGATGATGCTTTTGTTAAATTACCTAAAGTACCTACTGAATTATTTAAGTTAGTTGATGATAATGTCTTTCCTAAGTTAAATCCTAAGGGCTTAAATATCTCTGATAATATTGCTGCACTTTTTGAAAGATATAACCTTAAATCGATTGAACTTAAGAACTTTGACTTAGCTTTCTTGAGAAAAGCTGATGTAATTATCAAAGACAAGGTTCGATATAACTTTGAGATGCAAATGCAGTTTCAAACTGTTTATGTTGGTGGAGGGAATACCGTTATTAACTTAGACTTTACTTTAAAAGCCCAAACCGTTAACTTTGCTAACCTCCAAGATTTACAAAACACTTTTGTTAAAGTTGGTAATGATCTTTCCACCCAACTCTTTTGGATTCCAACTGTTAATAAATTAACTGATAATGCAGGTAATGATCTTACCCATATTGCTAAAACTGTGATTGGTGAATCGTTTTTCCAAACCAATGTTAACTTAGCTAAATCAGTTATTGAATATGATAAGGTTCAACCATTGGTTAAACAAGCTTTTGAAGAGCGAGTTTTAACTCCTTTCAAAAAGGAAAGAGAAGCTGCTAAAAAAGCTTATGAAGAAGAACAACGTCGCTTGGAAGAGGAACGTAAGCGTCAACTAGAAGAGCTAAGGAGAAGAGAAGCTGAGGAGAAAAGAAAAGCTGAAGAAGCAAAACGAAATCAAGAAAAAGCACGCAGAGAAAGAGAAGCTTATGAAAAATCATTTAACTCCTTTAAAGACTTTAAATTTTACTGGTTAACTAAAGGTAAAGATGTTACTAAAAAAGCTGATTTAATTGATGCACTTAAAACCGCTATTGCTACACCAGCATACAGAAATAGAACATTCTCTTTATTAATCAAAGGTTTTGCTAGTGGAGTTGAACGTTATTTCAACGCTAACAAGAATGATAAAGAGTTGAAAAAACTTGCATTTGGTGAAAAAGGGATCCAATTCCCTAGAGCAGATGCTGGGGTAAATGGTCTTTACATGAGTAATTTTTTGAGGCATGAATTGACAAGCAAAGCAAAATTTTCACTTAACCTAAAAGATATAAAAGTTGAAAATACGGTTGAAGATACTCAATTGTATTGGAAGGATAATGGCATTCATTTAAAGCAAGCCAATCCTTATAAATTCAATCTAAATATCAAGATCAAGTACAACGGCTGGTATAATGTCCACTGGTGA", "aa": "MKTQKNIFKVKALLLSLFSAAVTITIFALPIFANNGSKTDLGLLSKNSADFLGSSKRSLAGFDTPFSPDNLQYLEKETDYDQNFKSFTEKFKDEKITNNQLGIVDIYNLFSGFHKSVKSTVDLMNQLQKQVEAANAIFPVDDAFVKLPKVPTELFKLVDDNVFPKLNPKGLNISDNIAALFERYNLKSIELKNFDLAFLRKADVIIKDKVRYNFEMQMQFQTVYVGGGNTVINLDFTLKAQTVNFANLQDLQNTFVKVGNDLSTQLFWIPTVNKLTDNAGNDLTHIAKTVIGESFFQTNVNLAKSVIEYDKVQPLVKQAFEERVLTPFKKEREAAKKAYEEEQRRLEEERKRQLEELRRREAEEKRKAEEAKRNQEKARREREAYEKSFNSFKDFKFYWLTKGKDVTKKADLIDALKTAIATPAYRNRTFSLLIKGFASGVERYFNANKNDKELKKLAFGEKGIQFPRADAGVNGLYMSNFLRHELTSKAKFSLNLKDIKVENTVEDTQLYWKDNGIHLKQANPYKFNLNIKIKYNGWYNVHW", "aa_hexdigest": "5b9ebb73a9977879f73fcb541734dfc4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47342", "query_cov": 0.998158379373849, "subject_cov": 0.8325652841781874, "identity": 0.996, "score": 961.0, "evalue": 0.0, "valid": true, "product": "Uncharacterized protein MG096", "uniref50_id": "UniRef50_P47342" }, "pscc": { "uniref50_id": "UniRef50_P47342", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47342" ], "product": "Uncharacterized protein MG096" }, "genes": [], "id": "KKNAAMEMLJ_257", "locus": "GCA000027325_00218" }, { "type": "cds", "sequence": "contig_1", "start": 123254, "stop": 123523, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAATTTAACATTTGTTGTCAACGGTGACCTATCAGAAATTGTTGATAAACATGATTACCCTGGTACTTTCTTTCAATTTACCGATAAAAATGAATTGCTATTTACCTTAGCAGTTAGAGAACAAATTAAGGTTGATAATAATCATTTTATGGGTCTGTTAAAAAGCCAAAACCTTCATAATCTTCAGCTTGCTTCTGGTGCTACAAAACCTCCTGTTGTTGATTTAGCTAGTTATTTCCACTTTGTATTATTGACTGAAAAATCTTAA", "aa": "MNLTFVVNGDLSEIVDKHDYPGTFFQFTDKNELLFTLAVREQIKVDNNHFMGLLKSQNLHNLQLASGATKPPVVDLASYFHFVLLTEKS", "aa_hexdigest": "faa8681328b3bb4030f9cf2cf21a4b41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 47, "aa_cov": 0.5168539325842697, "hmm_cov": 1.0, "evalue": 3.323503311284362e-13, "score": 44.97151565551758, "start": 1, "stop": 46 } ], "seq_stats": { "molecular_weight": 10112.420499999991, "isoelectric_point": 5.675168800354004 }, "id": "KKNAAMEMLJ_258", "locus": "GCA000027325_00219" }, { "type": "cds", "sequence": "contig_1", "start": 124051, "stop": 124278, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAACTTTTCTAAAAAATCAAAACATTAAACCGCTGTTGGTATTTCTGGGTGTTCAAACTAAAAACTTTGTTGTTAAGAGTATTGGTAATGTTGATGGATTTGAGCATTTATCATATCCCCATCCCTCACCACTAAGTGGTAATTTGTTTCTAACAAACCCTAACGATCTGTTTAAAACAATTAACAATTGGTTGAAACAACATAACCAAAAAATAATTAACTGA", "aa": "MLTFLKNQNIKPLLVFLGVQTKNFVVKSIGNVDGFEHLSYPHPSPLSGNLFLTNPNDLFKTINNWLKQHNQKIIN", "aa_hexdigest": "39ebb89078017ab907b4aa6e583be638", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8589.877999999997, "isoelectric_point": 9.778645133972166 }, "id": "KKNAAMEMLJ_259", "locus": "GCA000027325_00220" }, { "type": "cds", "sequence": "contig_1", "start": 124417, "stop": 125154, "strand": "+", "frame": 1, "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "db_xrefs": [ "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681", "SO:0001217", "UniRef:UniRef50_P47344", "UniRef:UniRef90_P47344" ], "nt": "ATGGGAGAGATTAAAAATACTGCACCAACAAGCGATATTTCCACTTCAGGATTTATCTATTTTGCAGTTGTTTTTCTAATCATTATAGTCTATCTTTTTTTTAAAAACATTCTCTTTTTGTTCTTCTTTAAAAGATATCCTAAAAACACACCTAAAATTGGGGTTAGCAATATTACTACTATTGCTATGATTATTGCTGTTGCTGTATCTGTTGTTTTGGTCTTAATGGCTTTAGCAGGGGGGTTAACAGCAGCGCTGTTCCGTGGTTATCCTGGGTTCCGTGTTACCTTAGAGTTAATTCTAGTGAAGATTTCAGGACTTTTATTTGGTCCTATAATTGGTATTTTTTCAGCAGCTACCATTGACTTTTTAACTGTTATTTTTTCAGGTGGGGTGTTTAATATTGGTTATGTTTTAGGAGCAATCCTAACGGGAATGATTGCTGGAATTTTACGTGAAGTTTTAATTTCAACTTCTTTTTTAAATAACAAAACTTTAAGTGATTTTGCCTATCTTGTTTTATCAGTGGGGATGGTATTTGCCAGTTTTTTAGTAACCCAGTTTTTTGTTATCTCAGTTACCCAAAACTTATCAGCATTTCAAAGTAATGATCAAATTGTTTTACGTTTTAACGCCTCACCTTTAAATTTCAGTATCTCATTGCAAAGATATGTTCAAATTATTTTCTATTTTGCGATGGTTGTAATCATCACAATGGTAGTACTTTACTTTGTTTGA", "aa": "MGEIKNTAPTSDISTSGFIYFAVVFLIIIVYLFFKNILFLFFFKRYPKNTPKIGVSNITTIAMIIAVAVSVVLVLMALAGGLTAALFRGYPGFRVTLELILVKISGLLFGPIIGIFSAATIDFLTVIFSGGVFNIGYVLGAILTGMIAGILREVLISTSFLNNKTLSDFAYLVLSVGMVFASFLVTQFFVISVTQNLSAFQSNDQIVLRFNASPLNFSISLQRYVQIIFYFAMVVIITMVVLYFV", "aa_hexdigest": "5e54c5af3e0ccf8a3d223961fe32b9b2", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatC" ], "truncated": "3-prime", "pseudogene": { "start": 124417, "stop": 125457, "observations": { "mutation": [ 125152, 125374 ], "stop": [ 125152, 125374 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47344", "query_cov": 1.0, "subject_cov": 0.5136268343815513, "identity": 1.0, "score": 427.0, "evalue": 7.38e-147, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 245, "reference_sequence": "MGEIKNTAPTSDISTSGFIYFAVVFLIIIVYLFFKNILFLFFFKRYPKNTPKIGVSNITTIAMIIAVAVSVVLVLMALAGGLTAALFRGYPGFRVTLELILVKISGLLFGPIIGIFSAATIDFLTVIFSGGVFNIGYVLGAILTGMIAGILREVLISTSFLNNKTLSDFAYLVLSVGMVFASFLVTQFFVISVTQNLSAFQSNDQIVLRFNASPLNFSISLQRYVQIIFYFAMVVIITMVVLYFVWIIKQKHFNYAYSKFFFRRYKHANHQFTLFVLTKENWFYLILNVITLATTSLLMINIAFIPIFDTQTTGQTYDFWLLVRLLFAPLIFLLDIIVIYPILLLLTPIMLKGFKTVASETQTKGIKKSFSDMQSLIMPNVISHKKQQLIRKEMQQLAKTIRIDLSDKEVDALVEEFKEITKSFNKVTKIDTTNVQPMYAPFEFSPTPLRKDKPVVDKHAKQLLNNCCEVKTGFVKV" }, "paralog": false, "identity": 0.9942196531791907, "score": 655.0, "evalue": 5.32e-239, "description": "Internal stop codon at 125152, 125374. Nonsense mutation around 125152, 125374.", "psc": { "uniref90_id": "UniRef90_P47344", "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "uniref50_id": "UniRef50_P47344", "cog_id": "COG0721", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47344", "UniRef:UniRef50_P47344", "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P47344", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47344" ], "product": "Uncharacterized protein MG098" } }, "id": "KKNAAMEMLJ_260", "locus": "GCA000027325_00221" }, { "type": "cds", "sequence": "contig_1", "start": 125242, "stop": 125376, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACAAAAGAAAACTGGTTTTATCTAATTCTGAATGTAATTACATTAGCAACCACCAGTTTGCTAATGATTAACATTGCATTTATTCCTATCTTTGATACCCAAACAACTGGACAAACTTATGATTTTTGA", "aa": "MLTKENWFYLILNVITLATTSLLMINIAFIPIFDTQTTGQTYDF", "aa_hexdigest": "1ae888cae4e965623de8ebb0dcb8a1b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5116.9391000000005, "isoelectric_point": 4.0500284194946286 }, "id": "KKNAAMEMLJ_261", "locus": "GCA000027325_00222" }, { "type": "cds", "sequence": "contig_1", "start": 125392, "stop": 125850, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTCCTTTGATCTTCTTACTTGATATTATTGTTATCTATCCAATCTTGTTATTGTTAACCCCAATCATGTTAAAAGGGTTTAAAACAGTAGCATCAGAAACCCAAACAAAAGGAATCAAAAAGAGTTTTTCAGATATGCAAAGCTTAATTATGCCCAATGTTATTAGTCACAAAAAACAGCAGTTAATTAGAAAAGAGATGCAACAGTTAGCAAAAACAATCAGAATTGATTTATCAGACAAAGAAGTGGATGCACTTGTTGAAGAGTTTAAAGAGATCACAAAGAGTTTTAATAAGGTAACTAAAATTGATACCACTAATGTTCAACCGATGTACGCTCCATTTGAATTTAGTCCAACCCCACTAAGAAAAGATAAACCAGTAGTTGATAAACACGCTAAGCAACTACTTAATAACTGTTGTGAAGTTAAAACAGGTTTTGTAAAGGTATAG", "aa": "MFAPLIFLLDIIVIYPILLLLTPIMLKGFKTVASETQTKGIKKSFSDMQSLIMPNVISHKKQQLIRKEMQQLAKTIRIDLSDKEVDALVEEFKEITKSFNKVTKIDTTNVQPMYAPFEFSPTPLRKDKPVVDKHAKQLLNNCCEVKTGFVKV", "aa_hexdigest": "2f66afd55144866347b09c9b0c48e245", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17382.546599999998, "isoelectric_point": 9.416332817077638 }, "id": "KKNAAMEMLJ_262", "locus": "GCA000027325_00223" }, { "type": "cds", "sequence": "contig_1", "start": 125853, "stop": 126662, "strand": "+", "frame": 3, "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "db_xrefs": [ "COG:COG0154", "COG:J", "EC:6.3.5.7", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0030956", "GO:0050567", "SO:0001217", "UniRef:UniRef50_P47345", "UniRef:UniRef90_P47345" ], "nt": "GTGCGATCAAATATTTTAAGTCTCAGGGCGATACTTGATAAAAAACCTAGTGCCATTAACGATGTTTTAACATCAATTAATGCAAAGATAGAACTGAATAAATCAAGTAATTTTTTATTGAAAAATACTGTTGAAATTTATTCAAAAAAAATTAATAAAAGTGATGAAAAGATTCTGCTAAATAACATCCCTTATGTTTTGAAAGATAACATCGCTACTAAAGATATTGTCACCACTGGTGGTTCTTTGTTTTTAAAAAACTATCTTCCCCCTTTTTCAGCAACTGTGTTTGAACTGTTAGAAATGAATGGCGCGTTGCTTGTTGGTAAAGCTAATATGGATGAATTTGGCTTAGGTGGAACAGGTAGTTATTCTGCTTTTGGTGTTGTTCATCACCCTGAAAATTCCAGTTTAATTGCAGGTGGTTCTTCCTCAGGTTCAGCTTACGCAGTTGCTAAAGACATTGTTCCTTTTTCCATTGCAACTGACACTGGTGATTCGATTAGAAGACCTGCTAGTATCTGTAATGTTGTTGGCTTTAAACCAACTTATGGTTTGATATCACGTAATGGGGTATTTCCATATGCCCCAAGTATGGACCATGTGGGGATATTTGCTAAGTTTGTTAGTGATATTGCCATTGTTAGTGATGTTGTTATTAAACATGATAAAACTGATTTTTCTTCCCAAAAATCACCTGATGAAAACCAGTTTTTCAATGAGTTGGCCATTCCCTTTACAAGATCAATTCGCTTCGGTTATTTAAAACCACTAGAAAAACTGTTTAACAAACACCTCCAAAAAAAATGA", "aa": "MRSNILSLRAILDKKPSAINDVLTSINAKIELNKSSNFLLKNTVEIYSKKINKSDEKILLNNIPYVLKDNIATKDIVTTGGSLFLKNYLPPFSATVFELLEMNGALLVGKANMDEFGLGGTGSYSAFGVVHHPENSSLIAGGSSSGSAYAVAKDIVPFSIATDTGDSIRRPASICNVVGFKPTYGLISRNGVFPYAPSMDHVGIFAKFVSDIAIVSDVVIKHDKTDFSSQKSPDENQFFNELAIPFTRSIRFGYLKPLEKLFNKHLQKK", "aa_hexdigest": "cd92cb5d125a5d87cb7868e241d72642", "start_type": "GTG", "rbs_motif": null, "genes": [ "gatA" ], "truncated": "3-prime", "pseudogene": { "start": 125853, "stop": 126965, "observations": { "mutation": [ 126660 ], "stop": [ 126660 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47345", "query_cov": 1.0, "subject_cov": 0.5639412997903563, "identity": 1.0, "score": 517.0, "evalue": 7.86e-182, "gene_start": 1, "gene_end": 269, "reference_start": 1, "reference_end": 269, "reference_sequence": "MRSNILSLRAILDKKPSAINDVLTSINAKIELNKSSNFLLKNTVEIYSKKINKSDEKILLNNIPYVLKDNIATKDIVTTGGSLFLKNYLPPFSATVFELLEMNGALLVGKANMDEFGLGGTGSYSAFGVVHHPENSSLIAGGSSSGSAYAVAKDIVPFSIATDTGDSIRRPASICNVVGFKPTYGLISRNGVFPYAPSMDHVGIFAKFVSDIAIVSDVVIKHDKTDFSSQKSPDENQFFNELAIPFTRSIRFGYLKPLEKLFNKHLQKKWNNLKKTLEQKNYQLIPLDFDVELLKVIDSIYKIISYSEAVSCYSNLTGIVFGQKVFEPNSPSNFDQTITRNRDQFLGKQLKRRFVIGAFATDEKNFEKYFEKAQKIRRVLVDNFLNLFSDVDFVLSPSASCFASTIEDIQANKPYTNIIDDFLQLANFAGSPSITIPWLVQTKDQTIGLSISANCFEDKKLLQIAYWFEQLFDLNHD" }, "paralog": false, "identity": 0.9945945945945946, "score": 724.0, "evalue": 7.9e-266, "description": "Internal stop codon at 126660. Nonsense mutation around 126660.", "psc": { "uniref90_id": "UniRef90_P47345", "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "ec_ids": [ "6.3.5.7" ], "uniref50_id": "UniRef50_P47345", "cog_id": "COG0154", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0006412", "GO:0006424", "GO:0030956", "GO:0050567" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47345", "EC:6.3.5.7", "UniRef:UniRef50_P47345", "COG:COG0154", "COG:J", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0030956", "GO:0050567" ] }, "pscc": { "uniref50_id": "UniRef50_P47345", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47345" ], "product": "Glutamyl-tRNA(Gln) amidotransferase subunit A" } }, "id": "KKNAAMEMLJ_263", "locus": "GCA000027325_00224" }, { "type": "cds", "sequence": "contig_1", "start": 126702, "stop": 127253, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "TTGATTCCACTTGATTTTGATGTGGAACTTCTCAAAGTAATTGATTCTATTTACAAAATAATTAGTTATAGCGAAGCAGTTAGTTGTTATAGTAATTTAACTGGCATTGTCTTTGGTCAAAAGGTGTTTGAACCTAATTCACCAAGTAATTTTGATCAAACTATTACCAGAAACAGAGATCAGTTTTTAGGTAAACAACTAAAAAGAAGATTTGTAATAGGGGCATTTGCAACTGATGAGAAGAATTTTGAAAAGTACTTTGAAAAAGCTCAGAAAATAAGAAGAGTCTTAGTGGATAACTTTCTGAATCTCTTTAGTGATGTTGATTTTGTATTATCACCAAGCGCTTCTTGTTTTGCTAGTACCATTGAAGATATTCAAGCTAATAAGCCATATACAAACATTATTGATGACTTTTTACAATTAGCTAATTTTGCTGGTAGCCCTTCTATAACTATCCCTTGGTTAGTTCAAACAAAAGACCAAACAATTGGTTTAAGTATTAGTGCTAACTGTTTTGAAGATAAAAAACTCTTACAAATTGCTTATTGA", "aa": "MIPLDFDVELLKVIDSIYKIISYSEAVSCYSNLTGIVFGQKVFEPNSPSNFDQTITRNRDQFLGKQLKRRFVIGAFATDEKNFEKYFEKAQKIRRVLVDNFLNLFSDVDFVLSPSASCFASTIEDIQANKPYTNIIDDFLQLANFAGSPSITIPWLVQTKDQTIGLSISANCFEDKKLLQIAY", "aa_hexdigest": "ece9cb55301397cfdc6a817aa5ff7fe5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 180, "aa_cov": 0.9562841530054644, "hmm_cov": 0.9555555555555556, "evalue": 6.919056672724186e-31, "score": 102.76823425292969, "start": 5, "stop": 179 } ], "seq_stats": { "molecular_weight": 20807.522599999997, "isoelectric_point": 5.032376670837402 }, "id": "KKNAAMEMLJ_264", "locus": "GCA000027325_00225" }, { "type": "cds", "sequence": "contig_1", "start": 127279, "stop": 128712, "strand": "+", "frame": 1, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.-", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681", "KEGG:K02434", "RefSeq:WP_009885658.1", "SO:0001217", "UniParc:UPI000012B155", "UniRef:UniRef100_P47346", "UniRef:UniRef50_P75533", "UniRef:UniRef90_P47346" ], "nt": "ATGATTAATTTTGAAGCGATTATTGGAATTGAAGTCCATGTAGTTTTAAATACTGCTAGCAAGATGTTTTCAAAAGCACCTAACCGCGTTGATAATCAAAAAATCAATCATTTTATTGACCCAATAGATTTAGGTTTACCAGGCACTTTGCCTCAAGTTAATGAGTTAGCAGTTTACAAGGCATTATTATTAGCTGATGCATTAAAAATGAAGACAGTAACAAATAAACTTGTTTTTGATCGAAAGCACTATTTTTATCAGGACTTACCTAAGGGTTTTCAAATCACCCAACAAAATTATCCTTTTGCTAAAAATGGTGTAGTTACCATTAATGTTGATGCTATTGAAAAACCAATTTATATTGACCGGTTTCATTTGGAGGAAGATACTGCCAAACAACACTTTAACCATGACCAAATTCTGCTTGATTTTAATAGGTGTGGTGCACCTTTAATTGAAGTTGTTACTCTTCCTGTTATCAACACTGCAAAAGAAGCGAAAGCCTACCTACAGAAGTTGAGACAAATTCTGATTGTTAACAATATCTCCAATGCCAAATTGGAAGATGGTTCAATGCGGAGCGATTGCAATGTTTCAGTACGTTTAAAAGGTCAAAGGCAACTAGGAACTAAAATTGAAATTAAAAACATCAACTCACTTAATAATGTTGAAAAAGCGATTGATCTTGAGATTAACCGCCAAGTTAAAGCACTTATTAATGGTGAAACCTTGAGTCAAGCAACTTTAAGCTTTGATGATAAAACCAACAACAATGTTTTTATGAGAAAAAAAGACAATACGATTGACTATAGGTACTTTATTGAACCTAACATCATGACTAGTAATATTGATGATTTATTATTAGAAAAACCTGTTGCTTTTCAGTTAGAACAGTTTCAAAAAGAACTAATTGATAGTAATGTCAATCCTCAATTAGTCCAATTAGTAGTTGATGATGCAACTATCTTTAGTGCTTTTCAAACTATTAATAGTGTGATTAAAAACCCCCAAGAAACCATCAGGTGGTTATGTATTGAACTAATAGGTCAACTCAATAAAACCAACAGTTCATTAACAGCTAATTTAATTCAAGATCTAATTACCCTAATTGAAATGCTAAAAGCAGCAAAGGTTAACCAAAAACAAGCTAAGCAGTTAATTACTTTAATGATTGAAACTAAAAAAGATCCAAAATCGCTAGCTAAGCTCCATAATTTAGAGCAAATTACTGATCCAAAAGAGTTACAAAAGATCATTAAAAAAATCTTTCAGGAAAATGAAAAAGAGATCCTGAAAAACATTGATAGAATTGAACGGATTCAAAAGTTAATTATTGGTCAAGTTATGCATAAGACCAATAATCGAGCAAACCCCCAACAAGTTTTTATAATTGTTGAAAATATGCTTCATGAAGTTCGGGAAAGAGATAGCTAA", "aa": "MINFEAIIGIEVHVVLNTASKMFSKAPNRVDNQKINHFIDPIDLGLPGTLPQVNELAVYKALLLADALKMKTVTNKLVFDRKHYFYQDLPKGFQITQQNYPFAKNGVVTINVDAIEKPIYIDRFHLEEDTAKQHFNHDQILLDFNRCGAPLIEVVTLPVINTAKEAKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQRQLGTKIEIKNINSLNNVEKAIDLEINRQVKALINGETLSQATLSFDDKTNNNVFMRKKDNTIDYRYFIEPNIMTSNIDDLLLEKPVAFQLEQFQKELIDSNVNPQLVQLVVDDATIFSAFQTINSVIKNPQETIRWLCIELIGQLNKTNSSLTANLIQDLITLIEMLKAAKVNQKQAKQLITLMIETKKDPKSLAKLHNLEQITDPKELQKIIKKIFQENEKEILKNIDRIERIQKLIIGQVMHKTNNRANPQQVFIIVENMLHEVRERDS", "aa_hexdigest": "3b7ee13d7bce7bfc8125a9424777e913", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B155", "ncbi_nrp_id": "WP_009885658.1", "uniref100_id": "UniRef100_P47346", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B155", "RefSeq:WP_009885658.1", "UniRef:UniRef100_P47346" ] }, "ips": { "uniref100_id": "UniRef100_P47346", "uniref90_id": "UniRef90_P47346" }, "psc": { "uniref90_id": "UniRef90_P47346", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.-" ], "uniref50_id": "UniRef50_P75533", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P75533", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75533" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" }, "genes": [ "gatB" ], "id": "KKNAAMEMLJ_265", "locus": "GCA000027325_00226" }, { "type": "cds", "sequence": "contig_1", "start": 128906, "stop": 129355, "strand": "+", "frame": 2, "gene": "mngR", "product": "putative HTH-type transcriptional regulator MG101", "db_xrefs": [ "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700", "SO:0001217", "UniRef:UniRef50_P75532", "UniRef:UniRef90_P47347" ], "nt": "GTGTGTAAAGATGTAGGCTTTAGTTTTTTTCATAAAACCCAGGATAATCTGAAGGTGAAATATGCTAAACTTTCAACTTTGATTAAAAAGCTGTTATCACAGGATGATGCTAGTGTTTTTGCCAATATTGACAGTACTGTGCACCTTGATAAATTTAAGGGAATTGAAGCTAAGTTTTTTGATGAGAATAAAAAACACTTTTTAAACGTTTGTTTTTTTGCTAAAGATGATATTTTAAACATCCTTGATGAAAACAACCTCCAGCAACAGTTCTTCAGGGAGTTTGCTTACAATGGGATTGCTATTGAAAAACGTCATTGTGTTACCTCTGTTGACAAGGAAAGTGGGTGCTTGGTGATGTATGACATGTATTATGATGACAATGATAATTTTGTAGTTGCTAGCAAAAGTAACTTCCTTAACCCTGAATTGAAAGTAATCAATGCTTAA", "aa": "MCKDVGFSFFHKTQDNLKVKYAKLSTLIKKLLSQDDASVFANIDSTVHLDKFKGIEAKFFDENKKHFLNVCFFAKDDILNILDENNLQQQFFREFAYNGIAIEKRHCVTSVDKESGCLVMYDMYYDDNDNFVVASKSNFLNPELKVINA", "aa_hexdigest": "0a610266e88f07daa4cc4fff18420b8c", "start_type": "GTG", "rbs_motif": null, "genes": [ "mngR" ], "truncated": "5-prime", "pseudogene": { "start": 128687, "stop": 129355, "observations": { "mutation": [ 128765 ], "stop": [ 128765 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47347", "query_cov": 1.0, "subject_cov": 0.6711711711711712, "identity": 0.993, "score": 302.0, "evalue": 4.93e-103, "gene_start": 1, "gene_end": 149, "reference_start": 74, "reference_end": 222, "reference_sequence": "MKFGKEIAKKNQIIYRYIILKIQSFEWPANTRIFSERQLEIRFNSSRSQIRSVLATLLNKNIIRYTKNTPGYFVCKDVGFSFFHKTQDNLKVKYAKLSTLIKKLLSQDDASVFANIDSTVHLDKFKGIEAKFFDENKKHFLNVCFFAKDDILNILDENNLQQQFFREFAYNGIAIEKRHCVTSVDKESGCLVMYDMYYDDNDNFVVASKSNFLNPELKVINA" }, "paralog": false, "identity": 0.9954954954954955, "score": 442.0, "evalue": 1.71e-160, "description": "Internal stop codon at 128765. Nonsense mutation around 128765.", "psc": { "uniref90_id": "UniRef90_P47347", "gene": "MngR", "product": "Uncharacterized HTH-type transcriptional regulator MG101", "uniref50_id": "UniRef50_P75532", "cog_id": "COG2188", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003700" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47347", "UniRef:UniRef50_P75532", "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700" ] }, "pscc": { "uniref50_id": "UniRef50_P75532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75532" ], "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog" } }, "id": "KKNAAMEMLJ_266", "locus": "GCA000027325_00227" }, { "type": "cds", "sequence": "contig_1", "start": 129348, "stop": 130295, "strand": "+", "frame": 3, "gene": "trxB", "product": "thioredoxin-disulfide reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "EC:1.8.1.9", "GO:0004791", "GO:0005737", "GO:0019430", "GO:0045454", "GO:0071949", "RefSeq:WP_009885660.1", "SO:0001217", "UniParc:UPI00001376C6", "UniRef:UniRef100_P47348", "UniRef:UniRef50_P47348", "UniRef:UniRef90_P47348" ], "nt": "ATGCTTAAAGTGAATGCTGATTTTTTAACTAAAGATCAAGTTATCTATGATTTAGTGATAGTAGGTGCTGGCCCTGCTGGGATTGCTAGTGCCATTTATGGTAAACGTGCTAACTTAAATTTAGCAATTATTGAAGGAAACACTCCAGGAGGGAAGATAGTAAAAACTAACATTGTGGAAAACTATCCTGGTTTTAAAACCATAACTGGTCCTGAATTAGGTCTTGAGATGTACAACCACTTGTTAGCATTTGAACCAGTTGTTTTTTATAACAACTTAATCAAAATTGATCATCTTAACGATACATTCATCTTGTATTTAGATAACAAAACGACAGTTTTTAGCAAAACTGTTATCTATGCAACAGGGATGGAAGAGAGAAAACTTGGCATTGAAAAGGAAGATTATTTTTATGGTAAAGGGATTAGTTATTGTGCTATTTGTGATGCGGCTCTTTACAAAGGTAAAACAGTTGGTGTTGTAGGAGGAGGTAATTCTGCAATACAGGAAGCAATTTATCTTTCAAGTATTGCTAAAACAGTTCACCTTATTCACAGACGTGAAGTGTTTAGAAGTGATGCATTACTAGTTGAAAAATTAAAAAAAATTAGTAATGTAGTTTTTCATTTAAATGCTACTGTAAAACAGTTAATAGGTCAAGAAAAGCTCCAAACTGTTAAATTGGCAAGCACAGTTGATAAATCAGAAAGTGAAATTGCAATTGATTGTCTCTTTCCTTACATAGGCTTTGAAAGTAATAACAAGCCAGTTTTAGATCTTAAGCTTAATTTAGATCAAAATGGTTTTATTTTAGGAGATGAAAATATGCAAACTAACATTAAGGGTTTTTATGTTGCTGGGGATTGTAGAAGTAAATCATTCCGGCAAATTGCCACTGCAATTAGTGATGGGGTAACAGCTGTTTTAAAGGTTAGGGATGACATTTAG", "aa": "MLKVNADFLTKDQVIYDLVIVGAGPAGIASAIYGKRANLNLAIIEGNTPGGKIVKTNIVENYPGFKTITGPELGLEMYNHLLAFEPVVFYNNLIKIDHLNDTFILYLDNKTTVFSKTVIYATGMEERKLGIEKEDYFYGKGISYCAICDAALYKGKTVGVVGGGNSAIQEAIYLSSIAKTVHLIHRREVFRSDALLVEKLKKISNVVFHLNATVKQLIGQEKLQTVKLASTVDKSESEIAIDCLFPYIGFESNNKPVLDLKLNLDQNGFILGDENMQTNIKGFYVAGDCRSKSFRQIATAISDGVTAVLKVRDDI", "aa_hexdigest": "c62ad9a7e9d36b17e652e480cf0c7955", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001376C6", "ncbi_nrp_id": "WP_009885660.1", "uniref100_id": "UniRef100_P47348", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001376C6", "RefSeq:WP_009885660.1", "UniRef:UniRef100_P47348" ] }, "ips": { "uniref100_id": "UniRef100_P47348", "uniref90_id": "UniRef90_P47348" }, "psc": { "uniref90_id": "UniRef90_P47348", "gene": "trxB", "product": "thioredoxin-disulfide reductase", "ec_ids": [ "1.8.1.9" ], "uniref50_id": "UniRef50_P47348", "cog_id": "COG0492", "cog_category": "O", "go_ids": [ "GO:0004791", "GO:0005737", "GO:0019430", "GO:0045454", "GO:0071949" ] }, "pscc": { "uniref50_id": "UniRef50_P47348", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47348" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "KKNAAMEMLJ_267", "locus": "GCA000027325_00228" }, { "type": "cds", "sequence": "contig_1", "start": 130417, "stop": 131022, "strand": "+", "frame": 1, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "GO:0051301", "SO:0001217", "UniRef:UniRef50_P47349", "UniRef:UniRef90_P47349" ], "nt": "GTGCAATCTGAAGCATTAAAGGAACAATTTGTTCAAAATCTTAAGTTTGACTTTAAGACAAAAGCTAGTAAGAAATACTTTCTTTTTGAGTTTAATGCAGATATTAACGTAATTAACACTCTTTTAAAACTTGATGTGACAACTAGTGAATTGGTAGTTAAACAAGTTTATCTCATTGCTGCTTTTTTAAGTGGAGGTAGTGTTAGTGATTTAATAAACTCCAATAACTTTCACTTGCAAATCAGTTCCAACAATGAGTTTCAAATTCAACAACTTTTAAAATTGTTTAGTTTTTTTAAAAAAACAGTTAAACAAAACCAGTTAGTTGTTTATCTTAAAAGTTATGAAAAGATCTGTAATTTTTTAAAACTGATTCAAGCCTTTGATGGTTATCTTGCTTTTGAAAATAAGCAACTAGAGAAAAGTTTTACTTTAAACCAGTTAAGAAAAAGTAATTTGGAAGTTGCTAACTTAATGAAGACAATCAGATCTAATAATCAAACTAATCAACTCCAACTAAAATCATTTATTAAAAGTAGTAGTTTTGCAAAAAGACCGCTTAATTTTCAGCGTTATTGCTTAATTAAAAGTGATCATCCTGATTGA", "aa": "MQSEALKEQFVQNLKFDFKTKASKKYFLFEFNADINVINTLLKLDVTTSELVVKQVYLIAAFLSGGSVSDLINSNNFHLQISSNNEFQIQQLLKLFSFFKKTVKQNQLVVYLKSYEKICNFLKLIQAFDGYLAFENKQLEKSFTLNQLRKSNLEVANLMKTIRSNNQTNQLQLKSFIKSSSFAKRPLNFQRYCLIKSDHPD", "aa_hexdigest": "30fc90082c1df06ba3d8cd002482d3ca", "start_type": "GTG", "rbs_motif": null, "genes": [ "whiA" ], "truncated": "both", "pseudogene": { "start": 130285, "stop": 131127, "observations": { "mutation": [ 130345, 130405, 131020 ], "stop": [ 130345, 130405, 131020 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47349", "query_cov": 1.0, "subject_cov": 0.7178571428571429, "identity": 0.995, "score": 376.0, "evalue": 1.77e-130, "gene_start": 1, "gene_end": 201, "reference_start": 45, "reference_end": 245, "reference_sequence": "MTFSTQIKAELVQNKLIDKHWNVFLAGFFQNNLKLLYNRNWSFKVQSEALKEQFVQNLKFDFKTKASKKYFLFEFNADINVINTLLKLDVTTSELVVKQVYLIAAFLSGGSVSDLINSNNFHLQISSNNEFQIQQLLKLFSFFKKTVKQNQLVVYLKSYEKICNFLKLIQAFDGYLAFENKQLEKSFTLNQLRKSNLEVANLMKTIRSNNQTNQLQLKSFIKSSSFAKRPLNFQRYCLIKSDHPDWSLEQIANFFFTKYNIKISRSGIQHFSVNLKKLCQ" }, "paralog": false, "identity": 0.9892857142857143, "score": 536.0, "evalue": 7.45e-196, "description": "Internal stop codon at 130345, 130405, 131020. Nonsense mutation around 130345, 130405, 131020.", "psc": { "uniref90_id": "UniRef90_P47349", "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_P47349", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937", "GO:0051301" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47349", "UniRef:UniRef50_P47349", "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_P47349", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47349" ], "product": "Probable cell division protein WhiA" } }, "id": "KKNAAMEMLJ_268", "locus": "GCA000027325_00229" }, { "type": "cds", "sequence": "contig_1", "start": 131149, "stop": 131382, "strand": "+", "frame": 1, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020", "RefSeq:WP_010869333.1", "SO:0001217", "UniParc:UPI00001357BA", "UniRef:UniRef100_P58061", "UniRef:UniRef50_P58061", "UniRef:UniRef90_P58061" ], "nt": "ATGCATCCAATCCAAATAGTAATGTTCATTATGGCTGTTATCTGTTTAATTATTGGACTTTTGCTTTCTAACCATGGTTCTACTGGAGGATTAGCTTCTCTATCAGGTCAGGACTTGGAGATCTTTCGTAAAACCAAAGATAGGGGTTTTGTAAAGATCTTACAGATTATCATGTTTATCTTAGTAGTTTTATTTTTAATTCTTGGGTTGATATTTAGTTTTGCACCAAGATAA", "aa": "MHPIQIVMFIMAVICLIIGLLLSNHGSTGGLASLSGQDLEIFRKTKDRGFVKILQIIMFILVVLFLILGLIFSFAPR", "aa_hexdigest": "ae937ae76cf05dd412b9491e9bfeb245", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001357BA", "ncbi_nrp_id": "WP_010869333.1", "uniref100_id": "UniRef100_P58061", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001357BA", "RefSeq:WP_010869333.1", "UniRef:UniRef100_P58061" ] }, "ips": { "uniref100_id": "UniRef100_P58061", "uniref90_id": "UniRef90_P58061" }, "psc": { "uniref90_id": "UniRef90_P58061", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_P58061", "go_ids": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P58061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P58061" ], "product": "Probable protein-export membrane protein SecG" }, "genes": [ "secG" ], "id": "KKNAAMEMLJ_269", "locus": "GCA000027325_00230" }, { "type": "cds", "sequence": "contig_1", "start": 131385, "stop": 131546, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGTTTTAACTGAACTCCAAAAGCAGATATTTACCATTGTCAAAAAGGAAAATGGTAAACCTATTCCCCCTGGAATAGTGGTAAGAATGATGGAAAATAGTCCTAATTTTCCAGGTAAACATCTCATCTATCGGGCCATTGATGATCTGCTTGATTGA", "aa": "MKVLTELQKQIFTIVKKENGKPIPPGIVVRMMENSPNFPGKHLIYRAIDDLLD", "aa_hexdigest": "5a38b02da334b0cefc910e0643653a80", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6077.189500000001, "isoelectric_point": 9.22350788116455 }, "id": "KKNAAMEMLJ_270", "locus": "GCA000027325_00231" }, { "type": "cds", "sequence": "contig_1", "start": 131679, "stop": 132965, "strand": "+", "frame": 3, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_P47350", "UniRef:UniRef90_P47350" ], "nt": "TTGGATGATGATAAAACTGTGTATTATGTCCATTACTCTAATTTAACTGGAGCTTTAGATGGGGATCTTGTGGAGTTTTGTAAATTAGATAAACCCCAATTTGGTGATAAGTTTGATGCTGCAGTTATTACTATTCTAAAAAGAGCAAGAATCTTGTATGCAGGTAATTTTTTAGTAGATCAAAATGAGTTTGCCTTGGAATACAAAATTGTTGCTGATAACCCTAGATTTTATTTAACTATGATTGTAAATCCTGATTCTATCCCAAATAACTTAGCATCTAACACCAAGATAGCTTTTCAAATTGATGAGTATGATCCTGATAACAACTTATGTAAGGTTTCTGTACAACAAGTTTTGGGTAACAATGATGATCCGCTAATTAATATAAAAGCAATCATGTTGGACAATTCCATTGTCTTTGAAACTAACGATGTAGTTGAACAGCATGCTAACAAGTTAAGTTTTGATACTGAAGAACAACATAAAGCTTACCGTCAGGATTTAACTGATTTAGCTTTTGTGACTGTTGATCCTACAACATCAAAAGACCTTGATGATGCTATTTATGTCAAAACAATACCAACAGGTTTTGTGCTTTATGTAGCTATTGCTGATGTTGCACACTATGTTAATAGAAATAGTGAAATAGACATTGAAGCAAAACACAAAACAAGCTCAATCTATCTACCTGGTCATTATGTTGTGCCCATGCTACCTGAGCAATTGTCAAATCAGCTCTGTTCTTTAAATCCAGCACAAAAACGTTATGTTGTTGTTTGTGAGATTAGTTTTGATAATCAGGGAAGGATTAAAACAAACAAGCTTTACCCAGCAACAATTATTTCCAAAAATCGTTTTAGCTATGATCAGGTTAACAAGTGGTTAAATAATAAATCAGAATTAAACTGTGATGAAACAGTTATCAACAGCTTAAAAGCAGCTTTTACACTAAGTGATCTAATTCAAGCGCAACGTCAAAAACGCGGTACAATTGATCTTTCACACAAAGAAACTGAGATAGTTGTTGATGAACATTATTTTCCCATTAAGATAAATTTTTTGGTTCACGATAAAGCTGAAACCATGATTGAAAATCTCATGGTAGTGGCCAATGAGACAGTTGCTTGGGTGTTAACTAACAACAAAATTGCTTTACCATACAGAGTTCACCCAAGACCAAGCAAAAAGAAGTTACAAAGTTTGATTGAAACAGTTGGTGAGTTGAACATAACTAAACCCCAATTTAACTTAGATACTGTCACTTCAAGCCAAATAGCTAGCTGA", "aa": "MDDDKTVYYVHYSNLTGALDGDLVEFCKLDKPQFGDKFDAAVITILKRARILYAGNFLVDQNEFALEYKIVADNPRFYLTMIVNPDSIPNNLASNTKIAFQIDEYDPDNNLCKVSVQQVLGNNDDPLINIKAIMLDNSIVFETNDVVEQHANKLSFDTEEQHKAYRQDLTDLAFVTVDPTTSKDLDDAIYVKTIPTGFVLYVAIADVAHYVNRNSEIDIEAKHKTSSIYLPGHYVVPMLPEQLSNQLCSLNPAQKRYVVVCEISFDNQGRIKTNKLYPATIISKNRFSYDQVNKWLNNKSELNCDETVINSLKAAFTLSDLIQAQRQKRGTIDLSHKETEIVVDEHYFPIKINFLVHDKAETMIENLMVVANETVAWVLTNNKIALPYRVHPRPSKKKLQSLIETVGELNITKPQFNLDTVTSSQIAS", "aa_hexdigest": "e16b9443815c708c0748b6fe283c4e10", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "both", "pseudogene": { "start": 131385, "stop": 133268, "observations": { "mutation": [ 131544, 132963, 133146 ], "stop": [ 131544, 132963, 133146 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47350", "query_cov": 1.0, "subject_cov": 0.5729585006693441, "identity": 0.995, "score": 842.0, "evalue": 2.18e-303, "gene_start": 1, "gene_end": 428, "reference_start": 121, "reference_end": 548, "reference_sequence": "MGHHHHHHHHHHSSGHIDDDDKMKVLTELQKQIFTIVKKENGKPIPPGIVVRMMENSPNFPGKHLIYRAIDDLLDWAILRKAGGVTNQLLVNYEPAEPLLDKKLQGILTLGNKNSGFIRSLDDDKTVYYVHYSNLTGALDGDLVEFCKLDKPQFGDKFDAAVITILKRARILYAGNFLVDQNEFALEYKIVADNPRFYLTMIVNPDSIPNNLASNTKIAFQIDEYDPDNNLCKVSVQQVLGNNDDPLINIKAIMLDNSIVFETNDVVEQHANKLSFDTEEQHKAYRQDLTDLAFVTVDPTTSKDLADAIYVKTIPTGFVLYVAIADVAHYVNRNSEIDIEAKHKTSSIYLPGHYVVPMLPEQLSNQLCSLNPAQKRYVVVCEISFDNQGRIKTNKLYPATIISKNRFSYDQVNKWLNNKSELNCDETVINSLKAAFTLSDLIQAQRQKRGTIDLSHKETEIVVDEHYFPIKINFLVHDKAETMIENLMVVANETVAWVLTNNKIALPYRVHPRPSKKKLQSLIETVGELNITKPQFNLDTVTSSQIASWLNENKDNPSYEIFVILLLRTLGKAFYSVNPLMHFSIGSNHYTHFTSPIRRYIDLTIHRLLWMHLFTPDQFTDNERDQLKQELEKIADTVNDTEIKIINCERNANDYLTTLLLSKQIGKTFSGFISAITSFGIFMRMDENNFDGLIKITTIPDDFFIFEKEKMVLKGRKTNKVYKIGDRLEAKLSEIDFIQKRAILTLI" }, "paralog": false, "identity": 0.9936204146730463, "score": 1238.0, "evalue": 0.0, "description": "Internal stop codon at 131544, 132963, 133146. Nonsense mutation around 131544, 132963, 133146.", "psc": { "uniref90_id": "UniRef90_P47350", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_P47350", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47350", "EC:3.1.13.1", "UniRef:UniRef50_P47350", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ] }, "pscc": { "uniref50_id": "UniRef50_P47350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47350" ], "product": "Ribonuclease R" } }, "id": "KKNAAMEMLJ_271", "locus": "GCA000027325_00232" }, { "type": "cds", "sequence": "contig_1", "start": 133149, "stop": 133562, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00575.29" ], "nt": "ATGCATCTTTTTACTCCCGATCAATTCACTGATAATGAAAGAGATCAACTCAAACAAGAGTTGGAAAAAATTGCTGATACAGTTAATGATACAGAGATTAAAATTATCAATTGTGAAAGAAATGCCAATGATTATCTAACAACGCTGTTATTATCAAAACAAATTGGCAAAACCTTCAGCGGATTTATTTCAGCAATTACTAGCTTTGGAATTTTTATGAGAATGGATGAAAATAACTTTGATGGGTTAATCAAAATTACAACTATCCCTGATGATTTCTTTATTTTTGAAAAGGAAAAAATGGTATTGAAAGGAAGAAAAACTAATAAGGTTTATAAAATTGGCGATCGTTTGGAAGCTAAACTAAGTGAGATTGATTTCATCCAAAAACGTGCTATTTTAACACTCATATAA", "aa": "MHLFTPDQFTDNERDQLKQELEKIADTVNDTEIKIINCERNANDYLTTLLLSKQIGKTFSGFISAITSFGIFMRMDENNFDGLIKITTIPDDFFIFEKEKMVLKGRKTNKVYKIGDRLEAKLSEIDFIQKRAILTLI", "aa_hexdigest": "9bf5b7c9f3b55addc28ef91f1e12d655", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00575.29", "name": "S1", "length": 82, "aa_cov": 0.5985401459854015, "hmm_cov": 0.8536585365853658, "evalue": 5.0879901480416476e-12, "score": 41.166011810302734, "start": 54, "stop": 135 } ], "seq_stats": { "molecular_weight": 15918.203699999987, "isoelectric_point": 5.487600135803221 }, "id": "KKNAAMEMLJ_272", "locus": "GCA000027325_00233" }, { "type": "cds", "sequence": "contig_1", "start": 133570, "stop": 133686, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTTAATATCCTGCTTTTTATTACCCTTATTTTTTTACTTTTACTGTTTGTTTTTTTAATTGCTTTTGCATTTCTAAACAAACGGGTTAGAAATTATGTTGTACGTACTTGA", "aa": "MVVNILLFITLIFLLLLFVFLIAFAFLNKRVRNYVVRT", "aa_hexdigest": "e7bad74b7219d04b6fd7a3731452fcfd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4544.662900000002, "isoelectric_point": 11.722947120666507 }, "id": "KKNAAMEMLJ_273", "locus": "GCA000027325_00234" }, { "type": "cds", "sequence": "contig_1", "start": 133921, "stop": 134172, "strand": "+", "frame": 1, "gene": "cdaM", "product": "diadenylate cyclase CdaM", "db_xrefs": [ "COG:COG1624", "COG:T", "EC:2.7.7.85", "GO:0004016", "GO:0005524", "GO:0005886", "GO:0006171", "GO:0106408", "KEGG:K18672", "SO:0001217", "UniRef:UniRef50_P47351", "UniRef:UniRef90_P47351" ], "nt": "GTGATTGTTAGAGATTACAAAATTATTAGTGTCTCTTCTTACTTTCCAATGACAAGACAATTAATTGATGTTTCATATGGTTCAAGACACAGAAGTGCCTTGGGATTAAGTGAAAAGTCTGATGCAGTTGTTTTTATTGTTAGTGAAACAACTGGTAAAATATCAGTTGCTTTAAAAGGGGTTATTAAAACCTTGTCTTCCAACTCAGATAGGTTGCAAGATGAAATTATCCATTACTTGTCTTCAAAGTAG", "aa": "MIVRDYKIISVSSYFPMTRQLIDVSYGSRHRSALGLSEKSDAVVFIVSETTGKISVALKGVIKTLSSNSDRLQDEIIHYLSSK", "aa_hexdigest": "5729fce41ac549b731dc69104e4ea5cb", "start_type": "GTG", "rbs_motif": null, "genes": [ "cdaM" ], "truncated": "5-prime", "pseudogene": { "start": 133627, "stop": 134172, "observations": { "mutation": [ 133684 ], "stop": [ 133684 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47351", "query_cov": 0.9879518072289156, "subject_cov": 0.41, "identity": 1.0, "score": 155.0, "evalue": 2.94e-46, "gene_start": 2, "gene_end": 83, "reference_start": 119, "reference_end": 200, "reference_sequence": "MVVNILLFITLIFLLLLFVFLIAFAFLNKRVRNYVVRTWTSVFSKSKQNLDKKNFFDNLTSTLLRLSVDKIGAIIAIEKRDSLDPYINIGYRVSSDFSPELLVTIFYNKSSPLHDGAVIVRDYKIISVSSYFPMTRQLIDVSYGSRHRSALGLSEKSDAVVFIVSETTGKISVALKGVIKTLSSNSDRLQDEIIHYLSSK" }, "paralog": false, "identity": 0.994475138121547, "score": 343.0, "evalue": 4.09e-123, "description": "Internal stop codon at 133684. Nonsense mutation around 133684.", "psc": { "uniref90_id": "UniRef90_P47351", "gene": "cdaM", "product": "diadenylate cyclase CdaM", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_P47351", "cog_id": "COG1624", "cog_category": "T", "kegg_orthology_id": "K18672", "go_ids": [ "GO:0005886", "GO:0004016", "GO:0005524", "GO:0106408", "GO:0006171" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47351", "EC:2.7.7.85", "UniRef:UniRef50_P47351", "COG:COG1624", "COG:T", "KEGG:K18672", "GO:0005886", "GO:0004016", "GO:0005524", "GO:0106408", "GO:0006171" ] }, "pscc": { "uniref50_id": "UniRef50_P47351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47351" ], "product": "Diadenylate cyclase" } }, "id": "KKNAAMEMLJ_274", "locus": "GCA000027325_00235" }, { "type": "cds", "sequence": "contig_1", "start": 134516, "stop": 134728, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01327.26" ], "nt": "ATGACTTTTCAACCAACTAAAACTTGGCTTGTTTTTGATGATAATGCTTTGATTAACAAGCCAACTGAAGCTGTTAATTTTCCAATAGATGAGCAAATTGAAACCTGTATTAAAAAGATGATTGCATATGTTGATGCTTCATATGATGGTAAAGCACAAGAATATGACATTATTCCAGGAATTGGGATAGCTGCTAACCAGATTGGCTATTGA", "aa": "MTFQPTKTWLVFDDNALINKPTEAVNFPIDEQIETCIKKMIAYVDASYDGKAQEYDIIPGIGIAANQIGY", "aa_hexdigest": "f68adf1a996fd0d66b84e5bf1b67bbdd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01327.26", "name": "Pep_deformylase", "length": 61, "aa_cov": 0.8571428571428571, "hmm_cov": 0.8032786885245902, "evalue": 2.0495053437114665e-07, "score": 25.789291381835938, "start": 11, "stop": 70 } ], "seq_stats": { "molecular_weight": 7842.8357000000005, "isoelectric_point": 4.2393590927124025 }, "id": "KKNAAMEMLJ_275", "locus": "GCA000027325_00236" }, { "type": "cds", "sequence": "contig_1", "start": 134769, "stop": 135338, "strand": "+", "frame": 3, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163", "SO:0001217", "UniRef:UniRef50_P47353", "UniRef:UniRef90_P47353" ], "nt": "GTGAACAATCAGGGGAGAATTTTTGTCATTACTGGTCCTAGCGGTGTTGGCAAAAGCACCCTTGTTAAAGCCTTATTAGATCATTTCAAAGAACAACTGTTCTACAGTATCTCTGCAACTACAAGAAAAAAGCGCATTAGTGAAAAAGAGGGAATTGATTATTTTTTTAAAGATAAAGATGAGTTTGAAAACTTAATAAAACAAGATGCTTTCATTGAATGGGCTTGCTATAATAACCATTATTATGGAACGCTCAAGTCTCAAGCTGAACAAGCAATTAAAAGCGGAATTAATTTAATGCTTGAAATTGAGTATCAAGGTGCTTTACAGGTTAAAAGTAAATATCCTCATAACGTTGTTTTAATTTTCATTAAACCACCTTCAATGCAAGAGTTGTTAAAACGTTTAAAAAAGCGTAATGATGAAGATGAAACCACAATTAAAAAACGTTTAGAACAAGCTAAGATAGAGTTTCAACAGATTGATAATTTTAAGTATGTTGTCACTAACAAAGAGTTTGATAAAACCCTTAATGAGTTGAAATCAATCTTACTATCTGAGTTTATTTAA", "aa": "MNNQGRIFVITGPSGVGKSTLVKALLDHFKEQLFYSISATTRKKRISEKEGIDYFFKDKDEFENLIKQDAFIEWACYNNHYYGTLKSQAEQAIKSGINLMLEIEYQGALQVKSKYPHNVVLIFIKPPSMQELLKRLKKRNDEDETTIKKRLEQAKIEFQQIDNFKYVVTNKEFDKTLNELKSILLSEFI", "aa_hexdigest": "95bd95101c47f3b98b91effa28e4a3b9", "start_type": "GTG", "rbs_motif": null, "genes": [ "gmk" ], "truncated": "5-prime", "pseudogene": { "start": 134616, "stop": 135338, "observations": { "mutation": [ 134718 ], "stop": [ 134718 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47353", "query_cov": 1.0, "subject_cov": 0.7875, "identity": 0.995, "score": 367.0, "evalue": 1.71e-127, "gene_start": 1, "gene_end": 189, "reference_start": 52, "reference_end": 240, "reference_sequence": "MIQVSICSSIGKLTASVGLLIKALSSKTSQVLVGWKVIDKNSKVKIILTIEVNNQGRIFVITGPSGVGKSTLVKALLDHFKEQLFYSISATTRKKRISEKEGIDYFFKDKDEFENLIKQDAFIEWACYNNHYYGTLKSQAEQAIKSGINLMLEIEYQGALQVKSKYPHNVVLIFIKPPSMQELLKRLKKRNDEDETTIKKRLEQAKIEFQQIDNFKYVVTNKEFDKTLNELKSILLSEFI" }, "paralog": false, "identity": 0.9916666666666667, "score": 461.0, "evalue": 2.58e-167, "description": "Internal stop codon at 134718. Nonsense mutation around 134718.", "psc": { "uniref90_id": "UniRef90_P47353", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_P47353", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47353", "EC:2.7.4.8", "UniRef:UniRef50_P47353", "COG:COG0194", "COG:F", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_P47353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47353" ], "product": "Guanylate kinase" } }, "id": "KKNAAMEMLJ_276", "locus": "GCA000027325_00237" }, { "type": "cds", "sequence": "contig_1", "start": 135338, "stop": 135553, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTGCTAATGATCATTTCTTTACCGGTTTATCAAAAAAAGGTCCTGTAAGAAAGGAAAATCAGGACTTTTATGGATTTAGTTTTAATCAAAATAACCTACTAATAGTTGTTTGTGATGGTCTTGGTGGTTATAAGGGTGGCAAGATTGCTAGTAACTTAGTAGGTAAACTCTTTCTTAGTTTATTTGAAGGTTTTGAATTTAACCAGTGA", "aa": "MQAANDHFFTGLSKKGPVRKENQDFYGFSFNQNNLLIVVCDGLGGYKGGKIASNLVGKLFLSLFEGFEFNQ", "aa_hexdigest": "9d811b60877e9a5d207cc794be7488c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7867.837799999999, "isoelectric_point": 7.879471015930176 }, "id": "KKNAAMEMLJ_277", "locus": "GCA000027325_00238" }, { "type": "cds", "sequence": "contig_1", "start": 135806, "stop": 136120, "strand": "+", "frame": 2, "gene": "pTC1", "product": "Putative protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "GO:0007165", "GO:0017018", "GO:0033192", "GO:0140791", "GO:0180004", "GO:0180005", "GO:0180006", "GO:0180007", "GO:0180008", "GO:1990439", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_P47354", "UniRef:UniRef90_P47354" ], "nt": "ATGAATGCTGATGAGAAATTACTTTTAAGTTATTCTAATCAACTTTTAGCATTAACAAATACTATTTCCAAAGAAACAAAAAGACCATTAGTTTATGGTTTTTATAACACAAAAATTGAACAACAAGAATTTTTATTATTGTGTTCTGATGGACTTTATAACTTTGTTGAAAAAGAGCTTTTTTTTGAAATTATCACTAATTCTAAAAACCTAAAACAAGCAGTGTTTAATCTTTATAGAAAAAGTATTGAAAATGCAAGTAATGATAATATTACTGCTGCCCTTGTTAATCTGCAAAAATGGAAGCAATCTTAA", "aa": "MNADEKLLLSYSNQLLALTNTISKETKRPLVYGFYNTKIEQQEFLLLCSDGLYNFVEKELFFEIITNSKNLKQAVFNLYRKSIENASNDNITAALVNLQKWKQS", "aa_hexdigest": "bb93c639f64d7b55e5df02060884917c", "start_type": "ATG", "rbs_motif": null, "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 135506, "stop": 136120, "observations": { "mutation": [ 135551, 135716 ], "stop": [ 135551, 135716 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47354", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 204.0, "evalue": 1.15e-64, "gene_start": 1, "gene_end": 104, "reference_start": 157, "reference_end": 260, "reference_sequence": "MQAANDHFFTGLSKKGPVRKENQDFYGFSFNQNNLLIVVCDGLGGYKGGKIASNLVGKLFLSLFEGFEFNQWDETTVKKWFENTLIQARFQLENCFQTVYEAQIQFARMASTLVLGILTKSDIYIFWIGDSRAYLLFENQAKLVTKDHNLYNQLVAMNADEKLLLSYSNQLLALTNTISKETKRPLVYGFYNTKIEQQEFLLLCSDGLYNFVEKELFFEIITNSKNLKQAVFNLYRKSIENASNDNITAALVNLQKWKQS" }, "paralog": false, "identity": 0.9901960784313726, "score": 396.0, "evalue": 1.54e-142, "description": "Internal stop codon at 135551, 135716. Nonsense mutation around 135551, 135716.", "psc": { "uniref90_id": "UniRef90_P47354", "gene": "PTC1", "product": "Putative protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_P47354", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "go_ids": [ "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004", "GO:0007165" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47354", "EC:3.1.3.16", "UniRef:UniRef50_P47354", "COG:COG0631", "COG:T", "KEGG:K20074", "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004", "GO:0007165" ] }, "pscc": { "uniref50_id": "UniRef50_P47354", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47354" ], "product": "Putative protein phosphatase" } }, "id": "KKNAAMEMLJ_278", "locus": "GCA000027325_00239" }, { "type": "cds", "sequence": "contig_1", "start": 136105, "stop": 137028, "strand": "+", "frame": 1, "gene": "sPS1", "product": "Putative serine/threonine-protein kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "EC:2.7.11.1", "GO:0004674", "GO:0004676", "GO:0004677", "GO:0004679", "GO:0004711", "GO:0005524", "GO:0005886", "GO:0007165", "GO:0035175", "GO:0035402", "GO:0035403", "GO:0035979", "GO:0044022", "GO:0044023", "GO:0044024", "GO:0044025", "GO:0072354", "GO:0072371", "GO:0072518", "GO:0106310", "GO:0140823", "GO:0140855", "GO:0140857", "GO:1990244", "SO:0001217", "UniRef:UniRef50_P47355", "UniRef:UniRef90_P47355" ], "nt": "ATGGAAGCAATCTTAAAGATAGGTGATATTGTTGAAAATAAGTATCAAATTGAAAAGCTTTTAAATAGGGGTGGGATGGATTCCTATCTTTTTTTAGCAAAAAATTTGAACTTAAAAAACTATGGACCAGTTCAAAAAAAACAGTATGGACACTTAGTTTTAAAGGTGGTTCAAAAAAATCCTAAGATTAATGAAAATAATTGGAAAAAGTTCCTTGATGAAATGGTAACCACAACTAGAGTGCACCATTCTAACCTAGTGAAAAGTTTTGATGTGGTGAACCCTTTTTTGAAAATAGTTCGTGGTAATAAAACAATTGCTCTCAATCAGATAGTGATGATTGCAATGGAGTATGTTGATGGCCCTTCTTTAAGACAATTGCTTAACAGAAAAGGCTACTTTAGTGTTAGCGAAGTAGTTTATTACTTTACAAAGATAGTAAAAGCCATTGATTATCTCCACAGTTTCAAGCACCAAATTATCCACCGTGATCTTAAACCTGAAAACATCTTATTTACTAGTGATTTAACTGATATTAAACTATTAGATTTTGGCATTGCTTCAACAGTAGTTAAAGTTGCTGAAAAAACCGAAGTTTTAACTGATGAAAATTCACTGTTTGGCACAGTTAGTTATATGATTCCTGATGTTTTGGAAAGCACAGTTAATAAAGCTGGTAAAAAGGTTAGAAAACCACCCAATGCCCAGTATGATATCTACTCGTTAGGAATTATTCTATTTGAGATGTTAGTTGGTAGAGTTCCATTTAACAAGTCAATTAACCCCAACAAGGAAAGAGAAACAATCCAAAAAGCACGCAATTTTGACTTACCTTTAATGCAAGCAACTAGAAGTGATATCCCAAATAGCTTAGAAAATATTGCTTTTCGTTGTACTGCTGTGAAAAGAGAAAACAATAAGTGA", "aa": "MEAILKIGDIVENKYQIEKLLNRGGMDSYLFLAKNLNLKNYGPVQKKQYGHLVLKVVQKNPKINENNWKKFLDEMVTTTRVHHSNLVKSFDVVNPFLKIVRGNKTIALNQIVMIAMEYVDGPSLRQLLNRKGYFSVSEVVYYFTKIVKAIDYLHSFKHQIIHRDLKPENILFTSDLTDIKLLDFGIASTVVKVAEKTEVLTDENSLFGTVSYMIPDVLESTVNKAGKKVRKPPNAQYDIYSLGIILFEMLVGRVPFNKSINPNKERETIQKARNFDLPLMQATRSDIPNSLENIAFRCTAVKRENNK", "aa_hexdigest": "3ae8cd8ba63e45f2ed275564bb089a3d", "start_type": "ATG", "rbs_motif": null, "genes": [ "sPS1" ], "truncated": "3-prime", "pseudogene": { "start": 136105, "stop": 137268, "observations": { "mutation": [ 137026, 137071, 137182 ], "stop": [ 137026, 137071, 137182 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47355", "query_cov": 1.0, "subject_cov": 0.7932816537467701, "identity": 1.0, "score": 602.0, "evalue": 4.8e-216, "gene_start": 1, "gene_end": 307, "reference_start": 1, "reference_end": 307, "reference_sequence": "MEAILKIGDIVENKYQIEKLLNRGGMDSYLFLAKNLNLKNYGPVQKKQYGHLVLKVVQKNPKINENNWKKFLDEMVTTTRVHHSNLVKSFDVVNPFLKIVRGNKTIALNQIVMIAMEYVDGPSLRQLLNRKGYFSVSEVVYYFTKIVKAIDYLHSFKHQIIHRDLKPENILFTSDLTDIKLLDFGIASTVVKVAEKTEVLTDENSLFGTVSYMIPDVLESTVNKAGKKVRKPPNAQYDIYSLGIILFEMLVGRVPFNKSINPNKERETIQKARNFDLPLMQATRSDIPNSLENIAFRCTAVKRENNKWLYSSTKELLEDLANWENEQAMIKPANERVLEGQVEIREMMLEKPLAWYFKTWALSIFTIVFIGLIIAAIVLLLIFNARF" }, "paralog": false, "identity": 0.9922480620155039, "score": 748.0, "evalue": 4.39e-276, "description": "Internal stop codon at 137026, 137071, 137182. Nonsense mutation around 137026, 137071, 137182.", "psc": { "uniref90_id": "UniRef90_P47355", "gene": "SPS1", "product": "Putative serine/threonine-protein kinase", "ec_ids": [ "2.7.11.1" ], "uniref50_id": "UniRef50_P47355", "cog_id": "COG0515", "cog_category": "T", "go_ids": [ "GO:0005886", "GO:0004676", "GO:0004679", "GO:0005524", "GO:0004677", "GO:0044024", "GO:0072371", "GO:1990244", "GO:0035979", "GO:0044025", "GO:0140823", "GO:0035175", "GO:0044022", "GO:0140855", "GO:0035402", "GO:0072354", "GO:0140857", "GO:0035403", "GO:0044023", "GO:0106310", "GO:0004674", "GO:0072518", "GO:0004711", "GO:0007165" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47355", "EC:2.7.11.1", "UniRef:UniRef50_P47355", "COG:COG0515", "COG:T", "GO:0005886", "GO:0004676", "GO:0004679", "GO:0005524", "GO:0004677", "GO:0044024", "GO:0072371", "GO:1990244", "GO:0035979", "GO:0044025", "GO:0140823", "GO:0035175", "GO:0044022", "GO:0140855", "GO:0035402", "GO:0072354", "GO:0140857", "GO:0035403", "GO:0044023", "GO:0106310", "GO:0004674", "GO:0072518", "GO:0004711", "GO:0007165" ] }, "pscc": { "uniref50_id": "UniRef50_P47355", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47355" ], "product": "Putative serine/threonine-protein kinase" } }, "id": "KKNAAMEMLJ_279", "locus": "GCA000027325_00240" }, { "type": "cds", "sequence": "contig_1", "start": 137089, "stop": 137184, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAACCTGCTAATGAAAGGGTTTTAGAAGGGCAGGTGGAAATTAGAGAAATGATGCTTGAAAAGCCCTTAGCTTGGTATTTCAAAACTTGA", "aa": "MIKPANERVLEGQVEIREMMLEKPLAWYFKT", "aa_hexdigest": "c33c19896e15df6b6658bc9374a8b804", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3751.4417000000003, "isoelectric_point": 6.0999265670776355 }, "id": "KKNAAMEMLJ_280", "locus": "GCA000027325_00241" }, { "type": "cds", "sequence": "contig_1", "start": 137381, "stop": 137521, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGGTGATAGGGTTCAACTTGAAGATGGTGCGATCACTAAAGTTTTAGCTAGAAAAAATGAACTTACAAGACCAAGGGTTGCTAATGTTGATCAGATTGTTTTAATCCAATCACTAGTTCAACCTAAGATTAACTGA", "aa": "MVGDRVQLEDGAITKVLARKNELTRPRVANVDQIVLIQSLVQPKIN", "aa_hexdigest": "189b228caee193f8e7f40df2b8cfff7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5139.973, "isoelectric_point": 9.687615776062014 }, "id": "KKNAAMEMLJ_281", "locus": "GCA000027325_00242" }, { "type": "cds", "sequence": "contig_1", "start": 137531, "stop": 138058, "strand": "+", "frame": 2, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872", "KEGG:K06949", "SO:0001217", "UniRef:UniRef50_P75523", "UniRef:UniRef90_P47356" ], "nt": "TTGAAATTATTGGTATATTTCAATGCTAAATTAATTGATGAAATACCTATTTTAATAACAAAAACAGACCTTGATTTTGATCCAATGGAAAAACAAAAGTTAATCGATTTAAAACAGTTTAACTATCAGTTATTTTTTGTTTCTAAAAATGAACCACTACCTTCAGAATTAATCGATATTTTTAGTAAAAAACTAAGTGTTTTTACAGGTCAATCTGGTGTTGGTAAATCTAGCTTAATTAATCGTTTAGATCCTTCTTTAAAACAAAAGATTCAAGCCTTATCAGTTAATAAATTTGGTAAGAATACCACTACTAAAACAACACTTTTTTCATTTAGAGGCGGTTTTATTTGTGACACCCCTGGTTTTAATGTAATTTCTATTAAAAACCTCAAAATTTTAGCAGCCCAACACTTTGTTGGTTTTCAGAAAATGATTAGTAAGTGTCATTTTTCTAACTGTTATCATCAGTATGAAAAAGATTGCTTTGTAACCACAAGTGTTATGAAAAACAGATATCCTTCGTGA", "aa": "MKLLVYFNAKLIDEIPILITKTDLDFDPMEKQKLIDLKQFNYQLFFVSKNEPLPSELIDIFSKKLSVFTGQSGVGKSSLINRLDPSLKQKIQALSVNKFGKNTTTKTTLFSFRGGFICDTPGFNVISIKNLKILAAQHFVGFQKMISKCHFSNCYHQYEKDCFVTTSVMKNRYPS", "aa_hexdigest": "840f77a830b4db4270930cab4d57a428", "start_type": "TTG", "rbs_motif": null, "genes": [ "rsgA" ], "truncated": "both", "pseudogene": { "start": 137255, "stop": 138091, "observations": { "mutation": [ 137360, 137519, 138056 ], "stop": [ 137360, 137519, 138056 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47356", "query_cov": 1.0, "subject_cov": 0.6294964028776978, "identity": 0.9940000000000001, "score": 348.0, "evalue": 1.06e-119, "gene_start": 1, "gene_end": 175, "reference_start": 93, "reference_end": 267, "reference_sequence": "MPDSNFGIVLQSLAKQCKVFWNNQIITAFPQKKLQWKNDFKLMVGDRVQLEDGAITKVLARKNELTRPRVANVDQIVLIQSLVQPKINWIQLLKLLVYFNAKLIDEIPILITKTDLDFDPMEKQKLIDLKQFNYQLFFVSKNEPLPSELIDIFSKKLSVFTGQSGVGKSSLINRLDPSLKQKIQALSVNKFGKNTTTKTTLFSFRGGFICDTPGFNVISIKNLKILAAQHFVGFQKMISKCHFSNCYHQYEKDCFVTTSVMKNRYPSWLYEKYRKMIN" }, "paralog": false, "identity": 0.9892086330935251, "score": 543.0, "evalue": 3.24e-199, "description": "Internal stop codon at 137360, 137519, 138056. Nonsense mutation around 137360, 137519, 138056.", "psc": { "uniref90_id": "UniRef90_P47356", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.6.1.-" ], "uniref50_id": "UniRef50_P75523", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47356", "EC:3.6.1.-", "UniRef:UniRef50_P75523", "COG:COG1162", "COG:J", "KEGG:K06949", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75523" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" } }, "id": "KKNAAMEMLJ_282", "locus": "GCA000027325_00243" }, { "type": "cds", "sequence": "contig_1", "start": 138112, "stop": 138867, "strand": "+", "frame": 1, "gene": "pgi", "product": "glucose-6-phosphate isomerase", "db_xrefs": [ "COG:COG0166", "COG:G", "EC:5.3.1.9", "GO:0004347", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0048029", "GO:0051156", "GO:0097367", "SO:0001217", "UniRef:UniRef50_P47357", "UniRef:UniRef90_P47357" ], "nt": "ATGAGTGATAAATTATTAACAATTGACTTAAGTCATGTTTATGGATTTGATAAAGAAATTATTTTTAAGAAATACCAAAAAAAAGTAGATCAAATTCACCAAGATTTTCTAGCTCATAAACTTGCTGATGGTCACATGACTGGGTGGTATGACCAACCTGATCAAAACCACCAATTCCTTTTAAAAACCATTAATCAAATTGACAAAAAGTTTAAAAGTTTAAAAGTAACTGACATTGTTTATGTTGGTATTGGTGGTTCTTTTACTGGTATTAAAACAGTTTTAGATTTCTTAAAACCAAAACAAAGAACAGGATTAAAAATCCACTTTGTCCCTGACCTTTCTGCTTTTCAAGCTGCAAGTGTTATTAAGGAAATTAAAAATAAATCATGGGCTCTAATTACCACTTCTAAGTCTGGTAGAACCCTAGAACCAGCACTGAATTTCCGCATTTTTAGAAACTTATTAAACAAGCGTTATGGCAACAAACACTACCAAAGAGTAGTTGTTATTACTGATGAAAAAAAGGGATTACTAACCAAAATGGCATCAAATCATGGTTACCAAAAGTTAGTTATTGATTCAAATATCGGTGGGCGTTTTTCAACTCTATCTCCTGCTGGTTTGTTACTAGCCAAACTTTTTGGTCATGATCCTAAGGCCATCTTAAAAGGAACATTACAAGCCAAAAAGGATTTGCAAACAACTTCACTTGAAAACAATTCTGCATACCTTTATGCAGTAGTTAGACATTGA", "aa": "MSDKLLTIDLSHVYGFDKEIIFKKYQKKVDQIHQDFLAHKLADGHMTGWYDQPDQNHQFLLKTINQIDKKFKSLKVTDIVYVGIGGSFTGIKTVLDFLKPKQRTGLKIHFVPDLSAFQAASVIKEIKNKSWALITTSKSGRTLEPALNFRIFRNLLNKRYGNKHYQRVVVITDEKKGLLTKMASNHGYQKLVIDSNIGGRFSTLSPAGLLLAKLFGHDPKAILKGTLQAKKDLQTTSLENNSAYLYAVVRH", "aa_hexdigest": "07eef0244e1c0be1a3f0164ea79da9dd", "start_type": "ATG", "rbs_motif": null, "genes": [ "pgi" ], "truncated": "3-prime", "pseudogene": { "start": 138112, "stop": 139170, "observations": { "mutation": [ 138865 ], "stop": [ 138865 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47357", "query_cov": 1.0, "subject_cov": 0.5823665893271461, "identity": 1.0, "score": 501.0, "evalue": 9.23e-177, "gene_start": 1, "gene_end": 251, "reference_start": 1, "reference_end": 251, "reference_sequence": "MSDKLLTIDLSHVYGFDKEIIFKKYQKKVDQIHQDFLAHKLADGHMTGWYDQPDQNHQFLLKTINQIDKKFKSLKVTDIVYVGIGGSFTGIKTVLDFLKPKQRTGLKIHFVPDLSAFQAASVIKEIKNKSWALITTSKSGRTLEPALNFRIFRNLLNKRYGNKHYQRVVVITDEKKGLLTKMASNHGYQKLVIDSNIGGRFSTLSPAGLLLAKLFGHDPKAILKGTLQAKKDLQTTSLENNSAYLYAVVRHWLYTTKKFKIEVCIAYHSLYEYLLLQHRQLFGESEGKNDKSLFPTFSIFTVDLHSMGQLYQEGEKVFFETVIDVKNPLVNINLPPSDFDNDDELDFLLDKSLNEISDVAIDSVIKAHYQANVSIIKLTLKEQSAFMFGYFYFWLSVATVMSGSLLGHNVFNQPGVEVYKKLMFEKLRSGH" }, "paralog": false, "identity": 0.9971590909090909, "score": 703.0, "evalue": 1.18e-258, "description": "Internal stop codon at 138865. Nonsense mutation around 138865.", "psc": { "uniref90_id": "UniRef90_P47357", "gene": "pgi", "product": "glucose-6-phosphate isomerase", "ec_ids": [ "5.3.1.9" ], "uniref50_id": "UniRef50_P47357", "cog_id": "COG0166", "cog_category": "G", "go_ids": [ "GO:0004347", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0048029", "GO:0051156", "GO:0097367" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47357", "EC:5.3.1.9", "UniRef:UniRef50_P47357", "COG:COG0166", "COG:G", "GO:0004347", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0048029", "GO:0051156", "GO:0097367" ] }, "pscc": { "uniref50_id": "UniRef50_P47357", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47357" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "KKNAAMEMLJ_283", "locus": "GCA000027325_00244" }, { "type": "cds", "sequence": "contig_1", "start": 138919, "stop": 139293, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGAATATTTGTTATTACAGCATCGACAACTTTTTGGTGAATCAGAAGGTAAGAACGATAAATCTTTATTTCCTACTTTTTCGATTTTTACTGTTGACTTACACTCAATGGGACAACTCTATCAAGAAGGGGAAAAAGTGTTTTTTGAAACAGTAATTGATGTTAAAAATCCACTTGTTAATATTAATTTACCTCCATCTGATTTTGACAATGATGATGAACTTGATTTCTTGTTAGATAAAAGCTTAAATGAGATTTCAGATGTTGCAATTGATTCAGTTATTAAAGCGCACTACCAAGCAAATGTAAGCATTATTAAATTAACTTTAAAAGAACAATCTGCATTTATGTTTGGTTATTTTTACTTTTGA", "aa": "MYEYLLLQHRQLFGESEGKNDKSLFPTFSIFTVDLHSMGQLYQEGEKVFFETVIDVKNPLVNINLPPSDFDNDDELDFLLDKSLNEISDVAIDSVIKAHYQANVSIIKLTLKEQSAFMFGYFYF", "aa_hexdigest": "9c21bc0eb807d4b4ad1c0a13b1c0f4ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14374.081199999979, "isoelectric_point": 4.418231391906739 }, "id": "KKNAAMEMLJ_284", "locus": "GCA000027325_00245" }, { "type": "cds", "sequence": "contig_1", "start": 139397, "stop": 140026, "strand": "+", "frame": 2, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "EC:5.1.3.1", "GO:0004750", "GO:0005829", "GO:0005975", "GO:0009052", "GO:0019323", "GO:0046872", "RefSeq:WP_009885669.1", "SO:0001217", "UniParc:UPI0000134621", "UniRef:UniRef100_P47358", "UniRef:UniRef50_P47358", "UniRef:UniRef90_P47358" ], "nt": "GTGGCCACTAAGGTTGTTTTTTCACTCTTACCACTTTTAAATAGGTTTGACAAGTCACTTTTAGAAAGTTACTTTCAAGATGGATTGAGGTTAATCCATTATGATGTGATGGACCAATTTGTTCATAATACTGCTTTTAAAGGTGAATATTTGGATGAATTGAAAACAATAGGTTTTGATGTTAATGTCCATTTAATGGTGGAACAGATCATCCCTCAAATAAATTTTTATCTTTCACAACCTAATGTGAAAAGGATTTCGTTTCATGTTGAACCATTTAGTTTTGCAAAGATTAAAGAACTAATCCAACTAGTTAAAGAAAATGGTAAAGAAGTTGGTCTTGCTTTTAAATTTACAACCAATTTACAACTATACCAACCATTTTTTACAACCATCGACTTTATCACTTTAATGAGTGTTCCTCCTGGTAAAGGTGGTCAAGCTTTTAACGAAGCTGTTTTTACAAATTTAAAGATTGCTAACCATTACAACTTGAAAATTGAGATTGATGGTGGGATTAAAGTTAATAACATTGATCAAATTAAAGCCTTTGTTGATTTCATTGTAATGGGAAGTGGCTTTATAAAATTAGAGCAGTGGCAACGTCAAAAATTGTTGCAAACAATCTAA", "aa": "MATKVVFSLLPLLNRFDKSLLESYFQDGLRLIHYDVMDQFVHNTAFKGEYLDELKTIGFDVNVHLMVEQIIPQINFYLSQPNVKRISFHVEPFSFAKIKELIQLVKENGKEVGLAFKFTTNLQLYQPFFTTIDFITLMSVPPGKGGQAFNEAVFTNLKIANHYNLKIEIDGGIKVNNIDQIKAFVDFIVMGSGFIKLEQWQRQKLLQTI", "aa_hexdigest": "3e9c16fedfdc638449e854ff97b054d3", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134621", "ncbi_nrp_id": "WP_009885669.1", "uniref100_id": "UniRef100_P47358", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134621", "RefSeq:WP_009885669.1", "UniRef:UniRef100_P47358" ] }, "ips": { "uniref100_id": "UniRef100_P47358", "uniref90_id": "UniRef90_P47358" }, "psc": { "uniref90_id": "UniRef90_P47358", "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "ec_ids": [ "5.1.3.1" ], "uniref50_id": "UniRef50_P47358", "cog_id": "COG0036", "cog_category": "G", "go_ids": [ "GO:0005829", "GO:0004750", "GO:0046872", "GO:0005975", "GO:0019323", "GO:0009052" ] }, "pscc": { "uniref50_id": "UniRef50_P47358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47358" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [ "rpe" ], "id": "KKNAAMEMLJ_285", "locus": "GCA000027325_00246" }, { "type": "cds", "sequence": "contig_1", "start": 140040, "stop": 140273, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAAATCAGTTACAGTCAAGCAGTTACTACAAACCCCACGAAAATTTAATAACAAGCAGATTAAACTATCAGGTTGGGTTAAAAATAAACGTGCTAGTGCTAACATCATCTTTCTAGCAATTAGTGATGGCTCTAGTATTAATACCCTACAAGCAGTAGTAAAACAAGAAGATAACCCCCAGGTTTTCTCACTGTTACAAACTGTTAATTTAGCAAGTGCTGTTATGGTTTGA", "aa": "MKSVTVKQLLQTPRKFNNKQIKLSGWVKNKRASANIIFLAISDGSSINTLQAVVKQEDNPQVFSLLQTVNLASAVMV", "aa_hexdigest": "e9b27030ada31fc5733be86c96b6a892", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 47, "aa_cov": 0.5974025974025974, "hmm_cov": 0.9574468085106383, "evalue": 4.3986070536798994e-08, "score": 28.1107120513916, "start": 22, "stop": 67 } ], "seq_stats": { "molecular_weight": 8499.862000000001, "isoelectric_point": 10.461107444763183 }, "id": "KKNAAMEMLJ_286", "locus": "GCA000027325_00247" }, { "type": "cds", "sequence": "contig_1", "start": 140331, "stop": 141266, "strand": "+", "frame": 3, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_P47359", "UniRef:UniRef90_P47359" ], "nt": "GTGAGTTTATTAGCACAAGCAGAGTCTGATTATCCACTGCAAAAAAAAGAACATAGTCAAGAGTTTTTTAGAAGTAATGCGCATCTAAGAGTAAGAGCAAAAACTTACTTTGCAGTGATGAAAATAAGGAGTGTTTTGTCACACGCAATCTTTGAATACTTCTTTAAAAATGATTTTATCTTAGTGCAAAGCCCTATTTTAACTAGTAATGATTGTGAGGGAGCGGGGGAAACATTTGTAATTAAAGATAGTGAAACTTTTTTTAATAAAACGACTTTTTTAACAGTAAGTGGCCAGTTTGGAGCAGAAGCTTTTGCGCAAGCATTTAAAAAGGTTTTCACCTTTGGTCCTACTTTCAGAGCTGAAAAATCCCATACTAATCGTCATCTTAGTGAGTTTTGGATGATCGAACCTGAAATTGCATTTGCTAACTTAAAAGATTTAATGCAGTTAATACAAAACCTAATTAAATTCTTAATTAAAAAAGTGATGGAAAATGCTAGTGATGAACTAAATGTTTTAGCAAAGCAATTTAGCAATGACATTATTAGCAACTTAAAGACAATCATTAGTACTAAAAAATTTCCAATCATTGAATACAGCAAAGCATTAGCGATTCTAAAGGAATCTAGTGATACAAAAAAAACTAATTTTGAACTAAACGACTTTAGTTTTGGTATTGACTTAAAAACAGAACATGAACGCTTTTTGTGCGAACAATATTTTCAAAATCAACCGCTTTTTGTTATTAACTATCCAAAGGAGTTAAAGGCATTTTACATGAAAACAAATACTGACAATAAAACTGTTGCTGCAGTTGATCTTTTATTACCAAAGATTGGTGAGATTTGTGGGGGAAGTGAAAGGGAAAGTGATTTAAACCAACTTAAGAATAGGTGTCAATCTTTAAACATTGACACAAAAAGTTTGAACTGA", "aa": "MSLLAQAESDYPLQKKEHSQEFFRSNAHLRVRAKTYFAVMKIRSVLSHAIFEYFFKNDFILVQSPILTSNDCEGAGETFVIKDSETFFNKTTFLTVSGQFGAEAFAQAFKKVFTFGPTFRAEKSHTNRHLSEFWMIEPEIAFANLKDLMQLIQNLIKFLIKKVMENASDELNVLAKQFSNDIISNLKTIISTKKFPIIEYSKALAILKESSDTKKTNFELNDFSFGIDLKTEHERFLCEQYFQNQPLFVINYPKELKAFYMKTNTDNKTVAAVDLLLPKIGEICGGSERESDLNQLKNRCQSLNIDTKSLN", "aa_hexdigest": "c8604be61c724bce98994c13cdde93fc", "start_type": "GTG", "rbs_motif": null, "genes": [ "asnS" ], "truncated": "both", "pseudogene": { "start": 140040, "stop": 141410, "observations": { "mutation": [ 140271, 141264 ], "stop": [ 140271, 141264 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47359", "query_cov": 1.0, "subject_cov": 0.6820175438596491, "identity": 0.997, "score": 603.0, "evalue": 1.2e-215, "gene_start": 1, "gene_end": 311, "reference_start": 98, "reference_end": 408, "reference_sequence": "MKSVTVKQLLQTPRKFNNKQIKLSGWVKNKRASANIIFLAISDGSSINTLQAVVKQEDNPQVFSLLQTVNLASAVMVWGEIILTPKAKQPLELKLKQVSLLAQAESDYPLQKKEHSQEFFRSNAHLRVRAKTYFAVMKIRSVLSHAIFEYFFKNDFILVQSPILTSNDCEGAGETFVIKDSETFFNKTTFLTVSGQFGAEAFAQAFKKVFTFGPTFRAEKSHTNRHLSEFWMIEPEIAFANLKDLMQLIQNLIKFLIKKVMENASDELNVLAKQFSNDIISNLKTIISTKKFPIIEYSKALAILKESSDTKKTNFELNDFSFGIDLKTEHERFLCEQYFQNQPLFVINYPKELKAFYMKTNTDNKTVAAVDLLLPKIGEICGGSERESDLNQLKNRCQSLNIDTKSLNWYLDMRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRVHGTINF" }, "paralog": false, "identity": 0.9956140350877193, "score": 892.0, "evalue": 0.0, "description": "Internal stop codon at 140271, 141264. Nonsense mutation around 140271, 141264.", "psc": { "uniref90_id": "UniRef90_P47359", "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_P47359", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47359", "EC:6.1.1.22", "UniRef:UniRef50_P47359", "COG:COG0017", "COG:J", "KEGG:K01893", "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_P47359", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47359" ], "product": "Asparagine--tRNA ligase" } }, "id": "KKNAAMEMLJ_287", "locus": "GCA000027325_00248" }, { "type": "cds", "sequence": "contig_1", "start": 141276, "stop": 141410, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGAGGAAATGGGGTTATTTTGCTAGTGCAGGTTTTGGTTTGGGCTTTGATAGATTATTAGCTTATATATGTGGATTGGAAAACATCAGAGATGCTATTCCCTTTCCCCGTGTACATGGCACCATTAACTTCTAA", "aa": "MRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRVHGTINF", "aa_hexdigest": "fe5196cc5a7e35d0d9f2fd7fa4dfda18", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A378BFT2", "query_cov": 1.0, "subject_cov": 0.9166666666666666, "identity": 0.5, "score": 55.5, "evalue": 2.13e-09, "valid": false, "product": "Asparaginyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A378BFT2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 37, "aa_cov": 0.8409090909090909, "hmm_cov": 1.0, "evalue": 1.8286104419199824e-11, "score": 38.68692398071289, "start": 2, "stop": 38 } ], "seq_stats": { "molecular_weight": 4991.7507, "isoelectric_point": 9.183408546447755 }, "id": "KKNAAMEMLJ_288", "locus": "GCA000027325_00249" }, { "type": "cds", "sequence": "contig_1", "start": 141391, "stop": 141669, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACCATTAACTTCTAAATTCGCTGCTTATAAAAAAAAGATTGCAAACTGGTTAACAGTTTACAGAATTTTTATTGCTTTACCTACTATTATTTTTATTGCTTTAGATAATCAACTAGGAGTTTTAGCTAACTTTTCTGTTGGTGCAATTAGCATTAGTTTACAGATCAGTTTATTGATTGGAGGATTTTTGTTTTTAACTGCAGTTATATCAGATTATTTAGATGGATATTTAGCAAGAAAATGGCTAGCAGTTTCTAACTTTGGTAAATTATGA", "aa": "MAPLTSKFAAYKKKIANWLTVYRIFIALPTIIFIALDNQLGVLANFSVGAISISLQISLLIGGFLFLTAVISDYLDGYLARKWLAVSNFGKL", "aa_hexdigest": "243dd067f0be6358a5297264dec86aa4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10113.98109999999, "isoelectric_point": 9.82177448272705 }, "id": "KKNAAMEMLJ_289", "locus": "GCA000027325_00250" }, { "type": "cds", "sequence": "contig_1", "start": 141688, "stop": 141834, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATCAATGGTGTTCTTATTGCACTAGCGATTAATGGATATTTTCACTTTAGCTTATTAATTGTTTTTATAGTCCGTGATCTTGTGTTGGATGGAATGCGGATTTATGCTTATGAGAAAAAGGTGGTTATTGCTGCTAACTGA", "aa": "MIINGVLIALAINGYFHFSLLIVFIVRDLVLDGMRIYAYEKKVVIAAN", "aa_hexdigest": "ea3f372f1427cd7cb5a01ce016ab5adc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5411.513300000001, "isoelectric_point": 8.16035976409912 }, "id": "KKNAAMEMLJ_290", "locus": "GCA000027325_00251" }, { "type": "cds", "sequence": "contig_1", "start": 142081, "stop": 142554, "strand": "+", "frame": 1, "gene": "pncC", "product": "Protein MG115", "db_xrefs": [ "COG:COG1546", "COG:H", "EC:3.5.1.42", "KEGG:K03743", "RefSeq:WP_009885673.1", "SO:0001217", "UniParc:UPI00001392B9", "UniRef:UniRef100_P47361", "UniRef:UniRef50_P47361", "UniRef:UniRef90_P47361" ], "nt": "TTGTTTGCCAATTTAATAGCAGAAAAACTCCAAAAGTTACAACTTAGTGTTGCAACAGCTGAATCAGTTACTGGTGGCTTATTAGCTCATTGTTTAACTTCCATTGATGGTGCTTCTAACTATTTTAATGGCGGTGTTATTGCTTACAATAACCAAGTTAAGATTAACTTACTGAATGTTCAATCCTCCACAATTGCAAACCATGGGGCAGTTTCCAGTTTCTGTGCTAGGGAGATGGCAGTTGGGGTTAAACAAAAGTTTCAAGCTGATGTGGGTATTGCTTGCAGTGGGATAGCAGGTAGTAAAGCAGTTGAAAATAAAGCAATAGGATTACTTTTTTTCTGTATTATTATAGGAAATAAGGCTTATGATTTTGAGTTTGAAATGAACCAAAATAATCGTAAGGATAACATTGAATTATTTACCAATAAGATCTTGGAATCTTTCCACTATTTGTTAACAAAGCTAGCTTAA", "aa": "MFANLIAEKLQKLQLSVATAESVTGGLLAHCLTSIDGASNYFNGGVIAYNNQVKINLLNVQSSTIANHGAVSSFCAREMAVGVKQKFQADVGIACSGIAGSKAVENKAIGLLFFCIIIGNKAYDFEFEMNQNNRKDNIELFTNKILESFHYLLTKLA", "aa_hexdigest": "ee88f60c5e80f1baaaefcce45f207bc5", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001392B9", "ncbi_nrp_id": "WP_009885673.1", "uniref100_id": "UniRef100_P47361", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001392B9", "RefSeq:WP_009885673.1", "UniRef:UniRef100_P47361" ] }, "ips": { "uniref100_id": "UniRef100_P47361", "uniref90_id": "UniRef90_P47361" }, "psc": { "uniref90_id": "UniRef90_P47361", "gene": "PncC", "product": "Protein MG115", "ec_ids": [ "3.5.1.42" ], "uniref50_id": "UniRef50_P47361", "cog_id": "COG1546", "cog_category": "H", "kegg_orthology_id": "K03743" }, "pscc": { "uniref50_id": "UniRef50_P47361", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47361" ], "product": "Protein MG115" }, "genes": [ "pncC" ], "id": "KKNAAMEMLJ_291", "locus": "GCA000027325_00252" }, { "type": "cds", "sequence": "contig_1", "start": 142563, "stop": 143096, "strand": "+", "frame": 3, "gene": "ispD", "product": "putative protein MG116", "db_xrefs": [ "COG:COG1211", "COG:I", "SO:0001217", "UniRef:UniRef50_P75519", "UniRef:UniRef90_P47362" ], "nt": "ATGAAACGTACTTTAAATATAGGTATTGTTTTGTGTGAAAATTTTCTTTCAGACCAACAAAATGCTGTTGATAGTTACACCCAAGTTTATGAAGATGTTAGGATGTTTGAATTTGGGTTAAAACTCTTTCAATCATTACCTTTTAACATCCAAGAAACCCTTATATTTTGTAATGCTGAACAACATAAAATAGTTGATAAAGCTGCTAAAAAATACAAAAACACTACTGTGTTTTTTTCACGTGATACTGATGTAGCTAATGTTTATGAAGCTAAGGTATTTATCCAAGAAAAATACAAATTAACCCAAGATTACAAAAAAAGAGGTGTTAGCTCTTATTATGACAGCTGTTGCTTTATCTTAATTGAAGCGAATCGTCCATTAACTGCAATAAAGACAGTTAAAAGTGTTTATGAAAAAGCACTGATAGAAAAAGCAGCTATTGCAGTATTACCATATAATGGTACACTAATGAATGGTAACAATGATGTTGTCTTTAGCCACTTGCAAGATAAAAATAGGTTTAACTATTGA", "aa": "MKRTLNIGIVLCENFLSDQQNAVDSYTQVYEDVRMFEFGLKLFQSLPFNIQETLIFCNAEQHKIVDKAAKKYKNTTVFFSRDTDVANVYEAKVFIQEKYKLTQDYKKRGVSSYYDSCCFILIEANRPLTAIKTVKSVYEKALIEKAAIAVLPYNGTLMNGNNDVVFSHLQDKNRFNY", "aa_hexdigest": "f6ec4c09c526c983565036166bbab5d9", "start_type": "ATG", "rbs_motif": null, "genes": [ "ispD" ], "truncated": "3-prime", "pseudogene": { "start": 142563, "stop": 143318, "observations": { "mutation": [ 143094 ], "stop": [ 143094 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47362", "query_cov": 1.0, "subject_cov": 0.7051792828685259, "identity": 1.0, "score": 354.0, "evalue": 1.16e-122, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKRTLNIGIVLCENFLSDQQNAVDSYTQVYEDVRMFEFGLKLFQSLPFNIQETLIFCNAEQHKIVDKAAKKYKNTTVFFSRDTDVANVYEAKVFIQEKYKLTQDYKKRGVSSYYDSCCFILIEANRPLTAIKTVKSVYEKALIEKAAIAVLPYNGTLMNGNNDVVFSHLQDKNRFNYWKQSKAYEVQYPQAYTLNKLNQFSKQQFLRARSMLDLMKISNKSPLSIVDGSAYAFRVVTNLDFEILLGILKNG" }, "paralog": false, "identity": 0.9960159362549801, "score": 494.0, "evalue": 1.99e-180, "description": "Internal stop codon at 143094. Nonsense mutation around 143094.", "psc": { "uniref90_id": "UniRef90_P47362", "gene": "IspD", "product": "Uncharacterized protein MG116", "uniref50_id": "UniRef50_P75519", "cog_id": "COG1211", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47362", "UniRef:UniRef50_P75519", "COG:COG1211", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_P75519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75519" ], "product": "Uncharacterized protein MG116 homolog" } }, "id": "KKNAAMEMLJ_292", "locus": "GCA000027325_00253" }, { "type": "cds", "sequence": "contig_1", "start": 143193, "stop": 143318, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGACTTAATGAAGATTTCTAATAAATCACCCTTAAGTATTGTTGATGGTAGTGCTTATGCTTTTAGGGTTGTTACCAACCTTGATTTTGAAATTTTATTAGGTATTTTAAAAAATGGATAA", "aa": "MLDLMKISNKSPLSIVDGSAYAFRVVTNLDFEILLGILKNG", "aa_hexdigest": "8af0f9090e7692277155f57c0d623cce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4497.279299999998, "isoelectric_point": 5.91951961517334 }, "id": "KKNAAMEMLJ_293", "locus": "GCA000027325_00254" }, { "type": "cds", "sequence": "contig_1", "start": 143311, "stop": 143514, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAAATATTTTTCAATTAGTACAAAGTTTTGCTAAAACGCAAAATGTGCGAGCTAATTACACCTCACAAGACAAAAATATTAGTTTAGATTTTGTTAGTTTTGAAACAGTTAGTCAATCACTAACAGGTTTTCTAATTTTTAATAATTTCAATAAATTGTTACAACTAATTGAACTGATAAAACAAAAGCAAACATGA", "aa": "MDKNIFQLVQSFAKTQNVRANYTSQDKNISLDFVSFETVSQSLTGFLIFNNFNKLLQLIELIKQKQT", "aa_hexdigest": "695f58ce4a6841392ec802bbf66dbe4e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7789.825799999995, "isoelectric_point": 9.22402362823486 }, "id": "KKNAAMEMLJ_294", "locus": "GCA000027325_00255" }, { "type": "cds", "sequence": "contig_1", "start": 143602, "stop": 143955, "strand": "+", "frame": 1, "gene": null, "product": "MPN256 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47363", "UniRef:UniRef90_P47363" ], "nt": "TTGCCTGTTTCAGTTGCGGCTTTTAGCAACCAACAACTCAACCAAGTTTATCGCAATTCATCCACAAGTCCTTTGTATCTTAGTTATGTCAAACCAATCGAAGTTCAACAATTTTTTACACTTTCTCCATCCATTAACAATAATCCTTTTTTAAATCAAAATCCTTTAACTGAATCACCATTTGATAACAATAATCAGCTGTTTCAAGCAACAAAGAGCGTTGAACCTTCAATGGAAACAATGGAATTTTCCCGGTTTATGGATGAATTTGATCAGATTACAAAGAACTTTTCTGATATAGAGCTTGAACCTATGCAATTCACCCAAAGTTTTGATGATTGGAGCAAAGACTAG", "aa": "MPVSVAAFSNQQLNQVYRNSSTSPLYLSYVKPIEVQQFFTLSPSINNNPFLNQNPLTESPFDNNNQLFQATKSVEPSMETMEFSRFMDEFDQITKNFSDIELEPMQFTQSFDDWSKD", "aa_hexdigest": "20fe9c081e0b955f3813d61bd343d2d2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 143311, "stop": 143955, "observations": { "mutation": [ 143512, 143533 ], "stop": [ 143512, 143533 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47363", "query_cov": 1.0, "subject_cov": 0.5467289719626168, "identity": 0.991, "score": 229.0, "evalue": 8.6e-75, "gene_start": 1, "gene_end": 117, "reference_start": 98, "reference_end": 214, "reference_sequence": "MDKNIFQLVQSFAKTQNVRANYTSQDKNISLDFVSFETVSQSLTGFLIFNNFNKLLQLIELIKQKQTWLYVDQLWIVDLSNNKALTDATVWIIKQEKLPVSVAAFSNQQLNQVYRNSSTSPLYLSYVKPIEVQQFFTLSPSINNNPFLNQNPLTESPFDNNNQLFQATKSVEPSMETMEFSRFMDEFDQITKNFSDIELEPMQFTQSFDDWSKD" }, "paralog": false, "identity": 0.9906542056074766, "score": 417.0, "evalue": 2.96e-151, "description": "Internal stop codon at 143512, 143533. Nonsense mutation around 143512, 143533.", "psc": { "uniref90_id": "UniRef90_P47363", "product": "MPN256 family protein", "uniref50_id": "UniRef50_P47363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47363", "UniRef:UniRef50_P47363" ] }, "pscc": { "uniref50_id": "UniRef50_P47363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47363" ], "product": "Uncharacterized protein MG117" } }, "id": "KKNAAMEMLJ_295", "locus": "GCA000027325_00256" }, { "type": "cds", "sequence": "contig_1", "start": 143936, "stop": 144676, "strand": "+", "frame": 2, "gene": null, "product": "NAD-dependent epimerase/dehydratase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D", "UniRef:UniRef90_UPI0000557C9D" ], "nt": "ATGATTGGAGCAAAGACTAGGGTTGCAATAGTTGGCGGGATTGGTTACATAGGTAGTTGTTTTGCTAGTTTTATCAAAGAACAAAATGATAAGCTAATTGTTACTGTTATTGATAACAACAAAAATAACCATGTAATTAAACTCTTAAAAAAGATTGGAATTGAATTCTATTTTGCTGATTTACTAGATAGACATAAGCTAACTGAAGTAATTGCAGCAATTCAACCTGATGTGGTATTTCACTTTGCTGCTAAAACAAGTGTAAGTGAATCAGTACATAATCCATTGAAGTACTTTGATTGCAATGTAATTGGTACTTTAAACCTAATTAGTGCAATTAGTAACTTACAGAAGCCAATTAAATTATTTTTCGCTTCTAGTGCTGCAGTGTATGGTCAAACAACTAATAGTTACATTAGTGAAGAGATTGTAATAACTGAAACACAAGCAACCAATCCTTATGGATTGAGTAAGTTTTTAGATGAATTAATCTTAAATGCAGTTGCCAAAAATAGTCAACTACAAGTTGTTTGCTTACGCTTTTTTAATGTGGCAGGTGCAATTCTGCCATTTGGTAATTTTAATGGTAATACCACGCTTTTAATTCCTAACTTAGTAAAAGCCTTTTTAAAACAAACTCCCTTTTTTTTATATGGCAATGATTATGCAACTAAGGATGGTAGTTGCATAAGAGATTACATCCATGTTTATGATATATGTAATGCTCATTTCTTATTATGA", "aa": "MIGAKTRVAIVGGIGYIGSCFASFIKEQNDKLIVTVIDNNKNNHVIKLLKKIGIEFYFADLLDRHKLTEVIAAIQPDVVFHFAAKTSVSESVHNPLKYFDCNVIGTLNLISAISNLQKPIKLFFASSAAVYGQTTNSYISEEIVITETQATNPYGLSKFLDELILNAVAKNSQLQVVCLRFFNVAGAILPFGNFNGNTTLLIPNLVKAFLKQTPFFLYGNDYATKDGSCIRDYIHVYDICNAHFLL", "aa_hexdigest": "75b92aaa9fb3f53da333954460ea821d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557C9D", "query_cov": 0.8780487804878049, "subject_cov": 0.9432314410480349, "identity": 1.0, "score": 424.0, "evalue": 1.59e-149, "valid": true, "product": "NAD-dependent epimerase/dehydratase family protein", "uniref50_id": "UniRef50_UPI0000557C9D" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557C9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D" ], "product": "NAD-dependent epimerase/dehydratase family protein" }, "genes": [], "id": "KKNAAMEMLJ_296", "locus": "GCA000027325_00257" }, { "type": "cds", "sequence": "contig_1", "start": 144994, "stop": 146136, "strand": "+", "frame": 1, "gene": "nupO", "product": "Putative carbohydrate transport ATP-binding protein MG119", "db_xrefs": [ "COG:COG3845", "COG:F", "GO:0005524", "GO:0005886", "GO:0016887", "KEGG:K23537", "SO:0001217", "UniRef:UniRef50_P47365", "UniRef:UniRef90_P47365" ], "nt": "ATGGAGCATATCTCCAAAAGTTTTGACAATGGCAAAATTAAGGCTAATGTTGATGTTAGCTTAGTTGTTTATGAAAATACTGTCCACACCATTTTGGGGGAGAATGGTGCAGGAAAATCAACCCTGACTTCGATTTTATTTGGTTTATATAAACCTGATAGTGGCAAGATCTTTATTGGTGAAAAGCAAGTAAATTTTAAATCTTCTAAAGATGCAGTAAAACATAAAATCGGAATGGTGCACCAGCACTTTAAGTTAATAGAAAACTACACGGTTTTAGATAACATCATTCTAGGGAATGAAAGTAGGTTTGGGTTTTTACCTTTAATTAATCGTAAAGTAAGTGAAGCAAAGATTAAAACCATCATGGAAAAATATGGAATCTTTGTTGATCTTAAACAAAAAGTTAGTAACTTAACAGTAGGTCAGCAACAACGGGTTGAGATCCTAAAGGTTTTATTTCGTGATAGTAATATCCTTATCTTTGATGAACCCACTGCAGTTTTAAGTGATCTTGAAATTCAAAACTTTCTCAAGATTATTGCTAACTTTAAAAAGCTAGGAAAAACAATTGTTTTAATCTCTCATAAATTAAATGAAATTAAACAAGTTGCTGATACAGCTACTGTCTTAAGACTTGGCAAGGTAGTTGGTAGTTTTGATGTTAAAACAACACCAGTTGATAAGATTGCGCTTTTAATGATGGGCAAAGAGTTAAAACAAACTAAAAACACCACAGATTTTGTTGCTAAAGATGAACCTGTTTTAAAAGTTCAAAACCTGAATTTGTTTCTCAATAAATCTTTAGCATACAAGTTCTTAGTGAGGTGCAATAACATCCATAAAGCCCAACAAATTAAGAAAAATAAACCATTAAAAGACTTATGGATAATTAGTTTTTTAAATAAACTAACCACCAGTAACAAAACCCCTAAATTAGTAAAAGGCTTGATTAATAAGTTAGGACTTTCCTATCAAGAAAATACAGATGAAACCATTAGTTTTGCTATCCATAAGGGAGAAATTTTTGCTATTGCTGGGGTTGAGGGTAATGGTCAAAGTCAGCTTGTTAATTTAATTTGTGGAATTGAAAAAGCTGCTAGTAATAAGTTAATTTTTAACAATATTGATATCTCAAGATGA", "aa": "MEHISKSFDNGKIKANVDVSLVVYENTVHTILGENGAGKSTLTSILFGLYKPDSGKIFIGEKQVNFKSSKDAVKHKIGMVHQHFKLIENYTVLDNIILGNESRFGFLPLINRKVSEAKIKTIMEKYGIFVDLKQKVSNLTVGQQQRVEILKVLFRDSNILIFDEPTAVLSDLEIQNFLKIIANFKKLGKTIVLISHKLNEIKQVADTATVLRLGKVVGSFDVKTTPVDKIALLMMGKELKQTKNTTDFVAKDEPVLKVQNLNLFLNKSLAYKFLVRCNNIHKAQQIKKNKPLKDLWIISFLNKLTTSNKTPKLVKGLINKLGLSYQENTDETISFAIHKGEIFAIAGVEGNGQSQLVNLICGIEKAASNKLIFNNIDISR", "aa_hexdigest": "3bc627a29fdeab6d9e6a11687039c729", "start_type": "ATG", "rbs_motif": null, "genes": [ "nupO" ], "truncated": "3-prime", "pseudogene": { "start": 144973, "stop": 146439, "observations": { "mutation": [ 146134, 146269 ], "stop": [ 146134, 146269 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47365", "query_cov": 1.0, "subject_cov": 0.6737588652482269, "identity": 1.0, "score": 731.0, "evalue": 5.73e-263, "gene_start": 1, "gene_end": 380, "reference_start": 8, "reference_end": 387, "reference_sequence": "MEKVAFKMEHISKSFDNGKIKANVDVSLVVYENTVHTILGENGAGKSTLTSILFGLYKPDSGKIFIGEKQVNFKSSKDAVKHKIGMVHQHFKLIENYTVLDNIILGNESRFGFLPLINRKVSEAKIKTIMEKYGIFVDLKQKVSNLTVGQQQRVEILKVLFRDSNILIFDEPTAVLSDLEIQNFLKIIANFKKLGKTIVLISHKLNEIKQVADTATVLRLGKVVGSFDVKTTPVDKIALLMMGKELKQTKNTTDFVAKDEPVLKVQNLNLFLNKSLAYKFLVRCNNIHKAQQIKKNKPLKDLWIISFLNKLTTSNKTPKLVKGLINKLGLSYQENTDETISFAIHKGEIFAIAGVEGNGQSQLVNLICGIEKAASNKLIFNNIDISRWSIRKRINAGISFVLEDRHKYGLILDQTVRFNTVNNQINNRPFSSWNFLKPMEIALYSNTIIKKFDVRGSAEGSAVVRRLSGGNQQKLIIGREMTKQNDLLVLAQVTRGLDIGAIAFIHENILLAKANNKAILLVSYELDEILALADTVAVINKGRIVGMGKRDLMDRQSIGRLIMQ" }, "paralog": false, "identity": 0.9959016393442623, "score": 938.0, "evalue": 0.0, "description": "Internal stop codon at 146134, 146269. Nonsense mutation around 146134, 146269.", "psc": { "uniref90_id": "UniRef90_P47365", "gene": "NupO", "product": "Putative carbohydrate transport ATP-binding protein MG119", "uniref50_id": "UniRef50_P47365", "cog_id": "COG3845", "cog_category": "F", "kegg_orthology_id": "K23537", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47365", "UniRef:UniRef50_P47365", "COG:COG3845", "COG:F", "KEGG:K23537", "GO:0005886", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47365", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47365" ], "product": "Putative carbohydrate transport ATP-binding protein MG119" } }, "id": "KKNAAMEMLJ_297", "locus": "GCA000027325_00258" }, { "type": "cds", "sequence": "contig_1", "start": 146287, "stop": 146667, "strand": "+", "frame": 1, "gene": "mglA", "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "nt": "ATGGAGATTGCTCTTTATAGCAACACTATTATTAAAAAGTTTGATGTTAGGGGCAGTGCTGAGGGTAGTGCTGTTGTAAGAAGACTTTCAGGTGGTAATCAACAGAAACTAATTATTGGTCGAGAAATGACCAAACAAAATGACCTTTTGGTGTTAGCACAAGTAACCAGAGGCCTTGATATTGGTGCTATTGCTTTTATCCATGAAAACATCTTATTAGCTAAAGCTAATAATAAAGCTATCTTATTGGTTTCATATGAACTTGATGAGATCTTAGCACTTGCTGATACAGTGGCTGTTATCAATAAGGGGAGAATAGTTGGTATGGGAAAAAGAGATTTAATGGATCGCCAATCGATAGGTAGATTAATAATGCAATAA", "aa": "MEIALYSNTIIKKFDVRGSAEGSAVVRRLSGGNQQKLIIGREMTKQNDLLVLAQVTRGLDIGAIAFIHENILLAKANNKAILLVSYELDEILALADTVAVINKGRIVGMGKRDLMDRQSIGRLIMQ", "aa_hexdigest": "e869337ed1f0987f1d2aa27fd769dede", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M965", "query_cov": 0.9126984126984127, "subject_cov": 0.9274193548387096, "identity": 0.574, "score": 123.0, "evalue": 4.79e-34, "valid": false, "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "uniref50_id": "UniRef50_A0A223M965" }, "pscc": { "uniref50_id": "UniRef50_A0A223M965", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "product": "Galactose/methyl galactoside import ATP-binding protein MglA" }, "genes": [ "mglA" ], "id": "KKNAAMEMLJ_298", "locus": "GCA000027325_00259" }, { "type": "cds", "sequence": "contig_1", "start": 146758, "stop": 147198, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGAAGAAGTATCCTCTCTTCAGTGGTTTTGATAATCCTCTCTTTTCTTATATCGTTTTTACTGATTATTTCAATTCCTGGAGGTAGAGGTGCGAGCTTCTTTGCACTGTTTACTAAGTTATTTTTAGATAACACTAATACTGAAAATTTCTTAAGACAGATTGCTATTTATATCCTAGCTGGATTAGCATTTAGTTTCTGTATGAGTGTTGGTATTTTCAACATTGGTATCTCAGGGCAGATGATGGCTGGAGCCATCTTTGGGTTTTTAATGATTCTCAAGGTGTTTCCAAGTTCATTTCGACCTGGTTTTGGAGGTCAGATTATTACTGTATTATTGATGGTAATAGGTAGTGTTAGTGTGGCAGTTGTTGTTGCAACTTTAAAGATTTTTTTCAAGGTTAATGAAGTTGTAAGTGCAATTATGTTGAACTGA", "aa": "MQRRSILSSVVLIILSFLISFLLIISIPGGRGASFFALFTKLFLDNTNTENFLRQIAIYILAGLAFSFCMSVGIFNIGISGQMMAGAIFGFLMILKVFPSSFRPGFGGQIITVLLMVIGSVSVAVVVATLKIFFKVNEVVSAIMLN", "aa_hexdigest": "5029e9cca108206d566a5d1a98f2de81", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15812.016799999998, "isoelectric_point": 10.531829261779784 }, "id": "KKNAAMEMLJ_299", "locus": "GCA000027325_00260" }, { "type": "cds", "sequence": "contig_1", "start": 147205, "stop": 147339, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTATTAGTGCTTATTTAGTAGAGACTTACATTAAAGATAATAGTGGGGGTACAGCCCAATTCTTTTCCTTACCACTCCCTGATGAATTTGCTTTATATAACTTCTCTCCTTTAACAAAAAAGTTTGGTTGA", "aa": "MLISAYLVETYIKDNSGGTAQFFSLPLPDEFALYNFSPLTKKFG", "aa_hexdigest": "b0d6505bcbb722cd33230d78499be918", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.5999, "isoelectric_point": 4.780523109436035 }, "id": "KKNAAMEMLJ_300", "locus": "GCA000027325_00261" }, { "type": "cds", "sequence": "contig_1", "start": 147379, "stop": 147807, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCAGTAGTATTAAAATACACAGTTTTTGGACACAAATTAAAGTCAATTGGCAGTAGTGTATTTGGTTCTCAGGCAATGGGTTTTAATGTTAGAAAATACCAGTTCTTATCGTTTATTATCTCAGGAATTTTATCAGGACTATTAGCAACGGTTGTTTACACTGCATCAACTGAAAAAGTATTGACATTTAACAATGTTGGGGATAGTGCTATTTCAGCAGTACCAGCTACTGGTTTTGATGGGATTGCGATTGGTTTAATTGCTTTAAATAACCCCTTTAGGATTGTTATTGTTTCTGTTCTTATTGCTTTTGTTAACATTGGGGCAAGACCTGCTAATTTAAACCCTAATACTGCTAGTTTAGTTTTAGGAATCATGATGTATTTTGCTGCACTTTATAACCTAATGGTTTACTTTAAACCATGA", "aa": "MAVVLKYTVFGHKLKSIGSSVFGSQAMGFNVRKYQFLSFIISGILSGLLATVVYTASTEKVLTFNNVGDSAISAVPATGFDGIAIGLIALNNPFRIVIVSVLIAFVNIGARPANLNPNTASLVLGIMMYFAALYNLMVYFKP", "aa_hexdigest": "186e429f211fcf28888c01fadeded6c7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15146.773499999978, "isoelectric_point": 9.850978660583493 }, "id": "KKNAAMEMLJ_301", "locus": "GCA000027325_00262" }, { "type": "cds", "sequence": "contig_1", "start": 148054, "stop": 148236, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGCAATCCAAAGTGGCAATATTAAACCTTCAACTACTTTTAAGTTGGAATTTGTTAATTTTAAACACCAACAGAAGTTTGTATTAAATTGGTTTAAAAATGAAAGTGAATCACTGCGTGATTTCCAATCACAGTTTGAGAGAATCAATAAGTTAGTGGAAAGGGAGTTTGTTAAGTAA", "aa": "MNAIQSGNIKPSTTFKLEFVNFKHQQKFVLNWFKNESESLRDFQSQFERINKLVEREFVK", "aa_hexdigest": "e000d536240aea502ebf72ce98d2d85b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7282.212999999996, "isoelectric_point": 9.599294090270995 }, "id": "KKNAAMEMLJ_302", "locus": "GCA000027325_00263" }, { "type": "cds", "sequence": "contig_1", "start": 148239, "stop": 148436, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGTTTAGCACAATTAGAAAGTTGGTTTTTTATCGCTCCAGCACTGCTTTTAGCAGTATTGAGTGGTTATCTCGCTGAACGCGTTGGGATCATTAATATTGCTATTAATGGTGGAATGGTGTTTGGTGGGTTATTTATGGCACTCTTATCGTATGGATTTACAAATAACTTAAATCAATCCGCTCCCTCCTGA", "aa": "MLSLAQLESWFFIAPALLLAVLSGYLAERVGIINIAINGGMVFGGLFMALLSYGFTNNLNQSAPS", "aa_hexdigest": "fd18b1108f04c3f653ff47333939cb4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6908.0662999999995, "isoelectric_point": 4.529692649841309 }, "id": "KKNAAMEMLJ_303", "locus": "GCA000027325_00264" }, { "type": "cds", "sequence": "contig_1", "start": 148536, "stop": 148958, "strand": "+", "frame": 3, "gene": "nupQ", "product": "putative protein MG121", "db_xrefs": [ "COG:COG1079", "COG:F", "GO:0005886", "GO:0022857", "KEGG:K23536", "SO:0001217", "UniRef:UniRef50_P47367", "UniRef:UniRef90_P47367" ], "nt": "GTGGGAACTGGGATTAACTTGTTGGCTAGCGGAATTACCCTTTTTATTAGTCAGAATGCTGCTAGTTTGTTTTCCGATACTACCTTAAGAGTAAGGTACTTATTTCCCATCCAAACTACTGTTAGTATAGAAGCAATTGGTGTGTTTGTTTTTAGTTTACTTCTGATTGGGTTTGTATGGTACTTGATGAGTTTTACTAAAACTGGGTTGAGATACCGTGCGGTAGGTGAGAATCCTAATGTAATTGATACCCAAGGGATTAGTGTTTACAAATACCAATGGATAGGCGCAATTTGTTCAATGATGGTAGCTGGATTGAGTGGTAGTTTGTTTGTTTTAAGTGTTTCTAACTTTCCCTTTAACAGCGGAGATGTAAATGGCTTGGGTTTTATTGCTATTGCCATTATGATTATCTCAATGTGA", "aa": "MGTGINLLASGITLFISQNAASLFSDTTLRVRYLFPIQTTVSIEAIGVFVFSLLLIGFVWYLMSFTKTGLRYRAVGENPNVIDTQGISVYKYQWIGAICSMMVAGLSGSLFVLSVSNFPFNSGDVNGLGFIAIAIMIISM", "aa_hexdigest": "eca65363cbce6200ca83e86a8fd98295", "start_type": "GTG", "rbs_motif": null, "genes": [ "nupQ" ], "truncated": "both", "pseudogene": { "start": 148239, "stop": 149159, "observations": { "mutation": [ 148434, 148956 ], "stop": [ 148434, 148956 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47367", "query_cov": 1.0, "subject_cov": 0.45751633986928103, "identity": 0.993, "score": 266.0, "evalue": 7.84e-88, "gene_start": 1, "gene_end": 140, "reference_start": 100, "reference_end": 239, "reference_sequence": "MLSLAQLESWFFIAPALLLAVLSGYLAERVGIINIAINGGMVFGGLFMALLSYGFTNNLNQSAPSWSLFITIPLSVLFSSVIGCLFALAAVKLRADHVIVGTGINLLASGITLFISQNAASLFSDTTLRVRYLFPIQTTVSIEAIGVFVFSLLLIGFVWYLMSFTKTGLRYRAVGENPNVIDTQGISVYKYQWIGAICSMMVAGLSGSLFVLSVSNFPFNSGDVNGLGFIAIAIMIISMWRIIPSIFIGLIFAYAYVFTNSQIGSNSNSYLLRTIPFIISLLVMLLFGFLNVAPKNIGKHFDKGLR" }, "paralog": false, "identity": 0.9934640522875817, "score": 583.0, "evalue": 4.7e-215, "description": "Internal stop codon at 148434, 148956. Nonsense mutation around 148434, 148956.", "psc": { "uniref90_id": "UniRef90_P47367", "gene": "NupQ", "product": "Uncharacterized protein MG121", "uniref50_id": "UniRef50_P47367", "cog_id": "COG1079", "cog_category": "F", "kegg_orthology_id": "K23536", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47367", "UniRef:UniRef50_P47367", "COG:COG1079", "COG:F", "KEGG:K23536", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P47367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47367" ], "product": "Uncharacterized protein MG121" } }, "id": "KKNAAMEMLJ_304", "locus": "GCA000027325_00265" }, { "type": "cds", "sequence": "contig_1", "start": 149199, "stop": 149360, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAAACCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACATTAAAACAATATCTTCCTAGTGATGAATTTGAGATAGTCTCAACCGTTGGTCACATCAGAGAAATGGTGTATAAAAACTTTGGTTTTGATGAAAATACCTATACCCCTATCTGA", "aa": "MIKNLVVIESPNKVKTLKQYLPSDEFEIVSTVGHIREMVYKNFGFDENTYTPI", "aa_hexdigest": "70b39fdba60de8fd39e6d8f91219a05a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6177.061699999999, "isoelectric_point": 5.644021034240722 }, "id": "KKNAAMEMLJ_305", "locus": "GCA000027325_00266" }, { "type": "cds", "sequence": "contig_1", "start": 149529, "stop": 150968, "strand": "+", "frame": 3, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0551", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_P47368", "UniRef:UniRef90_P47368" ], "nt": "TTGGATCAAAAAGATAAAGCTAAGTGCAAACGAATCACTTTCAATGAGATCACTAAAAAAGCAGTAGTAGATGCATTAAAACAACCGCGTAACATCGATCTTAACTGGGTTGAAAGTCAGTTTGCCCGCCAAATCCTTGACAGGATGATAGGTTTTAGATTATCAAGATTATTAAATAGTTATCTGCAAGCAAAGTCTGCAGGTAGAGTTCAATCAGTGGCTTTGCGCTTTCTTGAGGAAAGAGAAAAGGAGATAGCTAAGTTTGTTCCGCGTTTTTGGTGGACAGTTGATGTTTTATTAAACAAAGAAAATAACCAAAAAGTAGTTTGTGCAAACAAGTCTATTCCTTTGGTTTTAAGAGAAATTAACCCTGAATTAAGTGCTAGTTTAAAACTGGATTTTGAAGCTGCTGAAAACGTATCAGGAATTGACTTTTTAAATGAAGCTTCAGCAACCAGATTTGCCAACCAACTGACTGGCGAATATGAAGTTTATTTTATTGATGAACCTAAGATTTACTATTCATCTCCAAACCCAGTTTATACCACCGCTTCACTTCAAAAGGATGCAATTAATAAGTTAGGATGGTCTTCCAAAAAAGTAACAATGGTGGCCCAAAGACTGTATGAAGGGATTAGTGTTAATGGGAAACAAACTGCATTAATTAGTTATCCAAGAACTGATTCAATTAGGATTTCAAACCAATTTCAATCAGAGTGTGAAAAGTACATTGAAAAGGAGTTTGGAAGTCATTATTTAGCTGATAAAAATAAGTTAAAAAGACATAAAAAGGATGAGAAAATCATCCAAGATGCCCATGAAGGGATCCATCCTACTTACATTACTATTACCCCCAATGATCTGAAAAACGGGGTGAAACGCGATGAGTTTCTCCTTTATCGTTTAATATGGATTAGAACAGTTGCTAGTTTAATGGCAGATGCTAAAACATCAAGAACTATTGTTCGTTTTATAAACCAAAAAAACAAGTTTTATACCTCTTCAAAATCACTTTTATTTGATGGTTATCAAAGGTTATATGAAGAGATTAAACCTAATACTAAAGATGAACTTTACATTGATCTTAGTAAGCTTAAAATTGGTGATAAATTTAGTTTTGAAAAGATCAGCGTTAATGAGCATAAAACCAACCCACCACCACGTTACACCCAAGCTAGTTTAATTGAAGAGCTTGAAAAATCTAACATCGGTCGTCCCTCTACTTATAACACTATGGCCAGTGTTAATCTAGAAAGGGGCTATGCTAACTTAGTGAACCGATTTTTTTATATCACTGAGCTTGGTGAAAAAGTTAATAATGAACTTTCCAAGCATTTTGGGAATGTAATTAATAAAGAATTTACCAAGAAGATGGAAAAATCTTTGGATGAAATTGCTGAAAACAAAGTAAACTATCAAGAATTTCTTAAGCAGTTTTGA", "aa": "MDQKDKAKCKRITFNEITKKAVVDALKQPRNIDLNWVESQFARQILDRMIGFRLSRLLNSYLQAKSAGRVQSVALRFLEEREKEIAKFVPRFWWTVDVLLNKENNQKVVCANKSIPLVLREINPELSASLKLDFEAAENVSGIDFLNEASATRFANQLTGEYEVYFIDEPKIYYSSPNPVYTTASLQKDAINKLGWSSKKVTMVAQRLYEGISVNGKQTALISYPRTDSIRISNQFQSECEKYIEKEFGSHYLADKNKLKRHKKDEKIIQDAHEGIHPTYITITPNDLKNGVKRDEFLLYRLIWIRTVASLMADAKTSRTIVRFINQKNKFYTSSKSLLFDGYQRLYEEIKPNTKDELYIDLSKLKIGDKFSFEKISVNEHKTNPPPRYTQASLIEELEKSNIGRPSTYNTMASVNLERGYANLVNRFFYITELGEKVNNELSKHFGNVINKEFTKKMEKSLDEIAENKVNYQEFLKQF", "aa_hexdigest": "c0057832d8c57db8df47d4aca2234c5a", "start_type": "TTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "both", "pseudogene": { "start": 149229, "stop": 151259, "observations": { "mutation": [ 149358, 149367, 150966 ], "stop": [ 149358, 149367, 150966 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47368", "query_cov": 1.0, "subject_cov": 0.6755994358251057, "identity": 0.998, "score": 937.0, "evalue": 0.0, "gene_start": 1, "gene_end": 479, "reference_start": 111, "reference_end": 589, "reference_sequence": "MIKNLVVIESPNKVKTLKQYLPSDEFEIVSTVGHIREMVYKNFGFDENTYTPIWEDWTKNKQKNPKQKHLLSKFEIIKSIKAKASDAQNIFLASDPDREGEAISWHVYDLLDQKDKAKCKRITFNEITKKAVVDALKQPRNIDLNWVESQFARQILDRMIGFRLSRLLNSYLQAKSAGRVQSVALRFLEEREKEIAKFVPRFWWTVDVLLNKENNQKVVCANKSIPLVLREINPELSASLKLDFEAAENVSGIDFLNEASATRFANQLTGEYEVYFIDEPKIYYSSPNPVYTTASLQKDAINKLGWSSKKVTMVAQRLYEGISVNGKQTALISYPRTDSIRISNQFQSECEKYIEKEFGSHYLADKNKLKRHKKDEKIIQDAHEGIHPTYITITPNDLKNGVKRDEFLLYRLIWIRTVASLMADAKTSRTIVRFINQKNKFYTSSKSLLFDGYQRLYEEIKPNTKDELYIDLSKLKIGDKFSFEKISVNEHKTNPPPRYTQASLIEELEKSNIGRPSTYNTMASVNLERGYANLVNRFFYITELGEKVNNELSKHFGNVINKEFTKKMEKSLDEIAENKVNYQEFLKQFWTNFKSDVKLAENSIQKVKKEKELVERDCPKCNQPLVYRYTKRGNEKFVGCSDFPKCKYSEFSNPKPKLTLETLDELCPECNNKLVKRRTKFNAKKTFIGCSNFPNCRFIKKDNAAEFKQ" }, "paralog": false, "identity": 0.9940828402366864, "score": 1280.0, "evalue": 0.0, "description": "Internal stop codon at 149358, 149367, 150966. Nonsense mutation around 149358, 149367, 150966.", "psc": { "uniref90_id": "UniRef90_P47368", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_P47368", "cog_id": "COG0551", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47368", "EC:5.6.2.1", "UniRef:UniRef50_P47368", "COG:COG0551", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47368", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47368" ], "product": "DNA topoisomerase 1" } }, "id": "KKNAAMEMLJ_306", "locus": "GCA000027325_00267" }, { "type": "cds", "sequence": "contig_1", "start": 151017, "stop": 151328, "strand": "+", "frame": 3, "gene": null, "product": "topoisomerase DNA-binding C4 zinc finger domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED90A12" ], "nt": "GTGAAAAAGGAAAAAGAATTGGTTGAAAGAGATTGTCCTAAATGTAATCAACCGTTGGTATATCGTTACACCAAAAGAGGTAATGAGAAGTTTGTTGGTTGTAGTGATTTTCCTAAGTGTAAATACAGTGAGTTTAGTAATCCTAAACCAAAACTAACCTTGGAAACACTTGATGAATTGTGTCCTGAGTGTAACAATAAACTGGTTAAGAGGAGAACTAAATTTAACGCTAAAAAGACCTTTATAGGTTGCAGTAATTTCCCTAACTGCCGTTTTATCAAAAAGGATAATGCTGCTGAATTTAAACAATAA", "aa": "MKKEKELVERDCPKCNQPLVYRYTKRGNEKFVGCSDFPKCKYSEFSNPKPKLTLETLDELCPECNNKLVKRRTKFNAKKTFIGCSNFPNCRFIKKDNAAEFKQ", "aa_hexdigest": "40f7b4a6aae3db1a0e228e74a2cd6e8f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED90A12", "query_cov": 0.8252427184466019, "subject_cov": 1.0, "identity": 0.8590000000000001, "score": 121.0, "evalue": 3.61e-34, "valid": false, "product": "topoisomerase DNA-binding C4 zinc finger domain-containing protein", "uniref50_id": "UniRef50_UPI001ED90A12" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED90A12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED90A12" ], "product": "topoisomerase DNA-binding C4 zinc finger domain-containing protein" }, "genes": [], "id": "KKNAAMEMLJ_307", "locus": "GCA000027325_00268" }, { "type": "cds", "sequence": "contig_1", "start": 151306, "stop": 152298, "strand": "+", "frame": 1, "gene": null, "product": "MPN262 family protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47369", "UniRef:UniRef90_P47369" ], "nt": "ATGCTGCTGAATTTAAACAATAACAGCGTTCTTATTGTTGCGTTTGTAATTGTTTCTTTATTCTTTCTAATAATTGTTGGGTTTGCTTTAAATTTAGCAATTGCTTTTTCACTCCATTTAAAGCAGAATAAAAATAACAAAAAATACATCTTAAATGACCAACAGATCCAGTTAAGATTAACTGAAAAACAAGCCCAATTAACAACTTTACTTAACTTTTATCAACAAAAAATTGAAAGTGTAAACAGAGAAAAAAGTTGGTTAGAAAGTCAGTTACAGGTAATTGATAAAAAGGATTTAAAGCAAGCGCAAAAGTTAACTTTACATTTAAAAAAAGACCAAATATTAGCTCAACTTAATGAAAAGCTGATTCAAAAAAAAGTTGATCAGCCTTTAGTTAATGAACTACAGAAAACCAAACTTTCCTATCTTGAAAGGTTAGTTGATCAAAAGATTAAACTCAGTGAAAATAATTTCAAAAGTGCTTTTCTTAAAACGAAGGTAAAAGAGACAGCATTTAATATCTTTGCAGCTAAAAACAAGGTGAACTGGGAGTATTTTAAACAGGTGTGTGATGCTGATTGCACTTTAAAAAACTTAGAAGATGAAGTGGAAATTACTTTTTCTAATTGGAGTTATTTGAGAAGGATGCAAGCTCTATTAGCGTTTGAAAAACTAATTAGCAAAATCAAAACAGTCAAAATTAATGAATTGGTTATTAATGAAACTTTAGATGAAGTGAAAAACGAAATTAGCCAAACTGCTTTTCAAGCGGGTGAAAAAATAGTTAAGGAATTTCAGATAACTAATTTAAACGAGCAAATTACCAGACTAATTGGCTTGCAAAAATACTATTTTGGCACTGATCAGCTCAATCTCTTAGAACTCGCGGTATTAACAACCAAATTAGTAATTTTACTGAACAAAAAGTTCAAACTAGATTTAGATTTAGAACTTCTTAAAGCAGCTAGCTTGTTTAACTATTTAAAGTGA", "aa": "MLLNLNNNSVLIVAFVIVSLFFLIIVGFALNLAIAFSLHLKQNKNNKKYILNDQQIQLRLTEKQAQLTTLLNFYQQKIESVNREKSWLESQLQVIDKKDLKQAQKLTLHLKKDQILAQLNEKLIQKKVDQPLVNELQKTKLSYLERLVDQKIKLSENNFKSAFLKTKVKETAFNIFAAKNKVNWEYFKQVCDADCTLKNLEDEVEITFSNWSYLRRMQALLAFEKLISKIKTVKINELVINETLDEVKNEISQTAFQAGEKIVKEFQITNLNEQITRLIGLQKYYFGTDQLNLLELAVLTTKLVILLNKKFKLDLDLELLKAASLFNYLK", "aa_hexdigest": "c8ad9fb56cf947bede4fc5699690847a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 151306, "stop": 152601, "observations": { "mutation": [ 152296 ], "stop": [ 152296 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47369", "query_cov": 1.0, "subject_cov": 0.7006369426751592, "identity": 1.0, "score": 597.0, "evalue": 1.21e-212, "gene_start": 1, "gene_end": 330, "reference_start": 1, "reference_end": 330, "reference_sequence": "MLLNLNNNSVLIVAFVIVSLFFLIIVGFALNLAIAFSLHLKQNKNNKKYILNDQQIQLRLTEKQAQLTTLLNFYQQKIESVNREKSWLESQLQVIDKKDLKQAQKLTLHLKKDQILAQLNEKLIQKKVDQPLVNELQKTKLSYLERLVDQKIKLSENNFKSAFLKTKVKETAFNIFAAKNKVNWEYFKQVCDADCTLKNLEDEVEITFSNWSYLRRMQALLAFEKLISKIKTVKINELVINETLDEVKNEISQTAFQAGEKIVKEFQITNLNEQITRLIGLQKYYFGTDQLNLLELAVLTTKLVILLNKKFKLDLDLELLKAASLFNYLKWVDNNQFFQILNTKLNQLLISDQVIAIIQQQELSFYPDQYGMLINGVKTMIREHNTIDFEKLVFLNSTKLIDNFYLYDLNMIHAVEYNNCFYYFVSVKPFEIKSLAELDLFVVLLKTFLAKKQKQNPKAVKLFITTKILAI" }, "paralog": false, "identity": 0.9976798143851509, "score": 831.0, "evalue": 3.28e-307, "description": "Internal stop codon at 152296. Nonsense mutation around 152296.", "psc": { "uniref90_id": "UniRef90_P47369", "product": "MPN262 family protein", "uniref50_id": "UniRef50_P47369", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47369", "UniRef:UniRef50_P47369", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47369", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47369" ], "product": "Uncharacterized protein MG123" } }, "id": "KKNAAMEMLJ_308", "locus": "GCA000027325_00269" }, { "type": "cds", "sequence": "contig_1", "start": 152419, "stop": 152721, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTAATGGAGTGAAAACAATGATTAGAGAGCATAACACCATTGATTTTGAAAAACTGGTTTTTCTCAACTCAACAAAACTTATTGATAATTTTTATCTCTATGATTTAAATATGATCCATGCAGTTGAATATAACAACTGCTTTTATTACTTTGTTAGTGTTAAACCCTTTGAGATTAAGTCATTAGCAGAACTTGATCTTTTTGTTGTTTTGTTAAAAACTTTTTTAGCTAAAAAGCAAAAGCAAAATCCAAAAGCGGTTAAACTGTTTATCACTACCAAAATTTTAGCGATCTAA", "aa": "MLINGVKTMIREHNTIDFEKLVFLNSTKLIDNFYLYDLNMIHAVEYNNCFYYFVSVKPFEIKSLAELDLFVVLLKTFLAKKQKQNPKAVKLFITTKILAI", "aa_hexdigest": "2a9f4881a699bb72b0bf3c1e1bd5ef9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11768.906399999985, "isoelectric_point": 9.212806129455569 }, "id": "KKNAAMEMLJ_309", "locus": "GCA000027325_00270" }, { "type": "cds", "sequence": "contig_1", "start": 153082, "stop": 153771, "strand": "+", "frame": 1, "gene": "cof", "product": "Putative phosphatase MG125", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "SO:0001217", "UniRef:UniRef50_P47371", "UniRef:UniRef90_P47371" ], "nt": "ATGATTGATCTGCTTGGTTTGGATCTGGATGGAACGTTATTATCTAAAACTAAAAAAATTAACAATCCATCAAAATTAGCATTAACTAATTTAATTGCTAAAAAACCAAGTTTAAAGGTGATGATTTTAACTGGTAGATCAGTTTTTTCTACTCTAAAACACGTTGAAAAGCTGAACAGTTTGTTTAAAAAACCAATTGTTGATTATTTTTGTTGTTATGGGGGTGCTAAACTTTATCAAATTGAAGCAAATAAGCCACAAGAAAGATACAAGTTTTGCTTGGAAAACAGTGTTGTTGAAACTACCTTTAGTATTATCAAAAAACACCGCGGATTATGTTTAGCTTACTTAGATAGTTATGTCTCTCCTTACCTTTGTTTAGCTGGTAACAAGCTCCTTGGGTGGTTCACTAAATACTTTTGGTATAGAAAAAGGTGTGTGTTTTTTAACCAGAACCATTTAAAACAAGGTATTCTAAAGATTAGTGTTTACTTTTTAAGTGCAAAAAGGTGTAAAAAAGTTTATGAAATCTTAAAAAATACCTTTCAAGAAAAGGTTAATGTTTTAAGTTTTTCTAATAATTTAATTGAGATAACTCATCATGATGCTAATAAGGGTTATGCAATTGAATATATGGCCAAAAGAGAACAACTTTCACTTAATAGAATAGCAGTTATTGGTGATTCTTGA", "aa": "MIDLLGLDLDGTLLSKTKKINNPSKLALTNLIAKKPSLKVMILTGRSVFSTLKHVEKLNSLFKKPIVDYFCCYGGAKLYQIEANKPQERYKFCLENSVVETTFSIIKKHRGLCLAYLDSYVSPYLCLAGNKLLGWFTKYFWYRKRCVFFNQNHLKQGILKISVYFLSAKRCKKVYEILKNTFQEKVNVLSFSNNLIEITHHDANKGYAIEYMAKREQLSLNRIAVIGDS", "aa_hexdigest": "4d2dd27c5f4598e07b4c9c74f5563782", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47371", "query_cov": 1.0, "subject_cov": 0.8035087719298246, "identity": 1.0, "score": 461.0, "evalue": 2.86e-163, "valid": true, "gene": "Cof", "product": "Putative phosphatase MG125", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P47371", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P47371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47371" ], "product": "Putative phosphatase MG125" }, "genes": [ "cof" ], "id": "KKNAAMEMLJ_310", "locus": "GCA000027325_00271" }, { "type": "cds", "sequence": "contig_1", "start": 153784, "stop": 153939, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAAAAGCTAAATATTCCTTTGCAATGTCAAAATCCCCTTCCCAGTTAAAATTAATTGCTACCAATACCAGTAACAAAACCAACCGTTACCGCTTTAGTACCTTACTTAATTTAATTAGTGAAACAATCATTAATCAAAAAGCTGATTAG", "aa": "MFKKAKYSFAMSKSPSQLKLIATNTSNKTNRYRFSTLLNLISETIINQKAD", "aa_hexdigest": "586c7c2a6f62f259930e18d13f136901", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5843.731199999998, "isoelectric_point": 10.29278049468994 }, "id": "KKNAAMEMLJ_311", "locus": "GCA000027325_00272" }, { "type": "cds", "sequence": "contig_1", "start": 153920, "stop": 154963, "strand": "-", "frame": 2, "gene": "trpS", "product": "tryptophan--tRNA ligase", "db_xrefs": [ "COG:COG0180", "COG:J", "EC:6.1.1.2", "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436", "RefSeq:WP_009885680.1", "SO:0001217", "UniParc:UPI0000136730", "UniRef:UniRef100_P47372", "UniRef:UniRef50_P75510", "UniRef:UniRef90_P47372" ], "nt": "ATGATAAAGCGCGCAATTACAGGGATTCAAGCTTCTGGAAGACAACACCTAGGTAACTTTCTTGGCGTAATGCAAGGTTTAAAACAACTCCAAAGTCAATACCAACTGTTTTTATTTGTTGCTGATCTTCATGCTATTACTGTTGATTTTGAACCAACAATGCTCAAAGATAACAACTTGCAACTTGTTAAAACTTTATTAGCACTAGGACTTGATTATGGAAAAGTGAACTTATTTTTACAAAGTGATCTGATGGAACATACCATGTTAGGTTATCTAATGCTGACACAAAGTAATCTAGGTGAATTACAAAGAATGACCCAATTTAAAACAAAGAAATTAGCGCAAAAAAGAAATAGTAATAACACCATTACTATCCCAACTGGTTTGTTAACTTACCCAGTGTTAATGGCTGCTGATATCTTGCTTTATCAACCTGATATTGTTCCAGTTGGTAATGATCAGAAGCAGCACTTGGAATTAACCAATGATTTAGCTAAACGTGTAGCAAAAAAATTTAAGTTAAAACTGAAATTACCTGTATTTATAGAAAACAAAGATACCAACAGGATCATGGATCTATCAAATCCTTTAAAAAAGATGTCCAAATCAAATCCTGATCAAAATGGTGTTATCTATCTGGATGATAGTAAAGAAACAATCATCAAAAAAGTGCGCAAAGCCACAACTGATAGTTTTAATAAGATTCGTTTTGCTAAAAAAACCCAACCTGGTGTTACTAATTTACTTGTTATTTTAACTGCACTTTTAAAAGAAGAAGTTAACCATAATTTAAGTAAAAAAATCGGCTCTGATCTTGTTAAATATTATCAGAATAAAAGTTATTTAGATTTAAAGAATGACCTCAGTAGTGCTGTTATTAATGTCATAGAATCACTTAAATTTAAAAAAGCACAAATTACTGATGAAATGGTATTAAAAGTCCTAAATGATGGTAAAAACCAAGCTAAAAAAGTTGCTGATGAAACATTAAAAATGTTTTATAAAGCATTTGGTTTAACATCTAATCAGCTTTTTGATTAA", "aa": "MIKRAITGIQASGRQHLGNFLGVMQGLKQLQSQYQLFLFVADLHAITVDFEPTMLKDNNLQLVKTLLALGLDYGKVNLFLQSDLMEHTMLGYLMLTQSNLGELQRMTQFKTKKLAQKRNSNNTITIPTGLLTYPVLMAADILLYQPDIVPVGNDQKQHLELTNDLAKRVAKKFKLKLKLPVFIENKDTNRIMDLSNPLKKMSKSNPDQNGVIYLDDSKETIIKKVRKATTDSFNKIRFAKKTQPGVTNLLVILTALLKEEVNHNLSKKIGSDLVKYYQNKSYLDLKNDLSSAVINVIESLKFKKAQITDEMVLKVLNDGKNQAKKVADETLKMFYKAFGLTSNQLFD", "aa_hexdigest": "662c716c8ef3f4c3e607474ef28e44f4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000136730", "ncbi_nrp_id": "WP_009885680.1", "uniref100_id": "UniRef100_P47372", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000136730", "RefSeq:WP_009885680.1", "UniRef:UniRef100_P47372" ] }, "ips": { "uniref100_id": "UniRef100_P47372", "uniref90_id": "UniRef90_P47372" }, "psc": { "uniref90_id": "UniRef90_P47372", "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_P75510", "cog_id": "COG0180", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_P75510", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75510" ], "product": "Tryptophan--tRNA ligase" }, "genes": [ "trpS" ], "id": "KKNAAMEMLJ_312", "locus": "GCA000027325_00273" }, { "type": "cds", "sequence": "contig_1", "start": 154999, "stop": 155436, "strand": "+", "frame": 1, "gene": "arsC", "product": "putative protein MG127", "db_xrefs": [ "COG:COG1393", "COG:P", "GO:0016491", "KEGG:K16509", "RefSeq:WP_010869345.1", "SO:0001217", "UniParc:UPI0000139302", "UniRef:UniRef100_P47373", "UniRef:UniRef50_P75509", "UniRef:UniRef90_P47373" ], "nt": "ATGAAAAAAGCGGGTAAAAAAAATAGTGATGCAGGTAAGACTTTTATCTTAATCTCATCATCCTGTTCATCCTGTCAGAAAGCCATTGAGTTTTTTGATCAAAACAAAATTAGCTATGTTGTTGAGAATTTTTATAAAAAACCAATTAGTGATAAGCGTTTTAAAGATATTTTAAGTCTTAGTGAGGATGGTACTGAAAGTTTGTTTTCCAAACGTGCTGATCAGATTAAAGCAACTAACAGTGTTAGTGTTGAAGAGTTGAGTATCAGTGAATTAATTAAGCTAGTTAGAGAACGTCCTTCTTTACTAAGAAGGCCTATTATCATCCAATATAATTCTTCAGGAATTCCTAAAAGGATGCGAATTGGTTATAACTCATCTGAAATTAAGGTCTTTGAGCGTAAACTAATAGAACCAAAGCCTATAATACAACAATAA", "aa": "MKKAGKKNSDAGKTFILISSSCSSCQKAIEFFDQNKISYVVENFYKKPISDKRFKDILSLSEDGTESLFSKRADQIKATNSVSVEELSISELIKLVRERPSLLRRPIIIQYNSSGIPKRMRIGYNSSEIKVFERKLIEPKPIIQQ", "aa_hexdigest": "40e8ac7b4528c13aeb66ae30ddcdf3a3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139302", "ncbi_nrp_id": "WP_010869345.1", "uniref100_id": "UniRef100_P47373", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139302", "RefSeq:WP_010869345.1", "UniRef:UniRef100_P47373" ] }, "ips": { "uniref100_id": "UniRef100_P47373", "uniref90_id": "UniRef90_P47373" }, "psc": { "uniref90_id": "UniRef90_P47373", "gene": "ArsC", "product": "Uncharacterized protein MG127", "uniref50_id": "UniRef50_P75509", "cog_id": "COG1393", "cog_category": "P", "kegg_orthology_id": "K16509", "go_ids": [ "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75509", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75509" ], "product": "Uncharacterized protein MG127 homolog" }, "genes": [ "arsC" ], "id": "KKNAAMEMLJ_313", "locus": "GCA000027325_00274" }, { "type": "cds", "sequence": "contig_1", "start": 155444, "stop": 156223, "strand": "+", "frame": 2, "gene": "nadK", "product": "NAD(+) kinase", "db_xrefs": [ "COG:COG0061", "COG:H", "EC:2.7.1.23", "GO:0003951", "GO:0005524", "GO:0005737", "GO:0006741", "GO:0019674", "GO:0046872", "GO:0051287", "KEGG:K00858", "RefSeq:WP_009885682.1", "SO:0001217", "UniParc:UPI00001320B7", "UniRef:UniRef100_P47374", "UniRef:UniRef50_P75508", "UniRef:UniRef90_P47374" ], "nt": "ATGAAATACAAAATTTTTGCTTCCACAACGCCCCAAACTGAACCTGTTCTCAATAAGTTAAGAGCAGTTTTAAAAACTTGGCAAGCGGTTGAGAATGGTTATGAATATGTGTTTGTTTTAGGTGGGGATGGTTTTTTTGTTTCCACACTTGCTAACTATAACTGTGATAGTTGTAAGGTAGTTGGTATCAATACTGGCCACATTGGTTTTTACACTTCTTTTAATGGAGATGATCTTGATGAAAATTTCATTTCAAAACTAACTAGCTTTGAGTTTAAAAAGATTAATTTACTGGAAGTGAAAACTAAAAACCACAGTTTCTTAGTTTTAAATGAACTTGCAGTTTACACCAACACTGCATATCCAATTAACATCTTTATTGATGATAACCACTGGGAATCATACCGCGGTTCAGGGTTGCTAATTGGCCCAAGAACAGGTTCAACTGCCCTAGCAAAATCTGCTAAGGGAGCGGTTATCTTTCCAAATGTTGATGTTGTTCAAATTATTGAACTAAACCCCTTATTACATCCCAACCAAATCACAATTCAATCTCCGATTATTCTGCCAATGCAAACCAAGGTTGAATTTAGGATCAAAAAAGCTTTTAAAGCAGAGCAATTTCCCAATTTTTATGCAGATGGAATCAAGCTTGATTTAAAAAATGAAGATACCAGTATTAGCTTTCAGTTGGTTTTATCAAGATCAATGTTTCACGCTTCTTTAAAAACTAAGGATTTTATTGATAAACTAAAATCAACTTTTATTAAGCAAAGCTAA", "aa": "MKYKIFASTTPQTEPVLNKLRAVLKTWQAVENGYEYVFVLGGDGFFVSTLANYNCDSCKVVGINTGHIGFYTSFNGDDLDENFISKLTSFEFKKINLLEVKTKNHSFLVLNELAVYTNTAYPINIFIDDNHWESYRGSGLLIGPRTGSTALAKSAKGAVIFPNVDVVQIIELNPLLHPNQITIQSPIILPMQTKVEFRIKKAFKAEQFPNFYADGIKLDLKNEDTSISFQLVLSRSMFHASLKTKDFIDKLKSTFIKQS", "aa_hexdigest": "066db6882e51e126553a606259001cdf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001320B7", "ncbi_nrp_id": "WP_009885682.1", "uniref100_id": "UniRef100_P47374", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001320B7", "RefSeq:WP_009885682.1", "UniRef:UniRef100_P47374" ] }, "ips": { "uniref100_id": "UniRef100_P47374", "uniref90_id": "UniRef90_P47374" }, "psc": { "uniref90_id": "UniRef90_P47374", "gene": "nadK", "product": "NAD(+) kinase", "ec_ids": [ "2.7.1.23" ], "uniref50_id": "UniRef50_P75508", "cog_id": "COG0061", "cog_category": "H", "kegg_orthology_id": "K00858", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ] }, "pscc": { "uniref50_id": "UniRef50_P75508", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75508" ], "product": "NAD kinase" }, "genes": [ "nadK" ], "id": "KKNAAMEMLJ_314", "locus": "GCA000027325_00275" }, { "type": "cds", "sequence": "contig_1", "start": 156241, "stop": 156576, "strand": "+", "frame": 1, "gene": "ptsG2", "product": "Putative phosphotransferase enzyme IIB component MG129", "db_xrefs": [ "COG:COG1264", "COG:G", "EC:2.7.1.-", "GO:0008982", "GO:0009401", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75507", "UniRef:UniRef90_Q49397" ], "nt": "TTGGGTTATATTTTTAGTTTTGGTTTACTCTATCTTTGGATAGTAAAAAAATCAAAACAAATTGCTCAACAACCTAACACAAAACTGGTTGAATCAACTTCTATTCCTTTTAAAGTTAAGGACTTTGTTAGTGCTTGTGGTGGTAAGGAAAATTTTGTTAATATAAAAACAACACCAACTCAATTAATAGTTACTTTTAAAGATGTTAACTCAGTGAGCTTAACAAAGCTTAATGCGCTTAATATCAAAGGAATTAACAAAAACCAAAACCAATTTCGTTTTGTACTTGGTAACTTTGTAAATGAATTGAAAAAAAAGATAGAAGATGAACAATAA", "aa": "MGYIFSFGLLYLWIVKKSKQIAQQPNTKLVESTSIPFKVKDFVSACGGKENFVNIKTTPTQLIVTFKDVNSVSLTKLNALNIKGINKNQNQFRFVLGNFVNELKKKIEDEQ", "aa_hexdigest": "33b1d2bd36b0289eaebe26ed1a156f46", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49397", "query_cov": 1.0, "subject_cov": 0.9487179487179487, "identity": 0.991, "score": 216.0, "evalue": 4.16e-71, "valid": true, "gene": "PtsG2", "product": "Putative phosphotransferase enzyme IIB component MG129", "ec_ids": [ "2.7.1.-" ], "uniref50_id": "UniRef50_P75507", "cog_id": "COG1264", "cog_category": "G", "go_ids": [ "GO:0016020", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75507", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75507" ], "product": "Putative phosphotransferase enzyme IIB component MPN_268" }, "genes": [ "ptsG2" ], "id": "KKNAAMEMLJ_315", "locus": "GCA000027325_00276" }, { "type": "cds", "sequence": "contig_1", "start": 156566, "stop": 157858, "strand": "+", "frame": 2, "gene": "rny", "product": "Ribonuclease Y", "db_xrefs": [ "COG:COG1418", "COG:JR", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0005886", "GO:0006402", "SO:0001217", "UniRef:UniRef50_P47376", "UniRef:UniRef90_P47376" ], "nt": "ATGAACAATAACATTACTAACAGTATTGCCCAATTGTTTTTCAACACTTCTTTCTTTGCTTTTCTGTTTTTAATCATTATTGCGTTTAATTTATGCTTGTTTGCCTATCTTTATTTTCAGTACCGAATTTATAAGAAAAACCCTAAAAAAGCTAACAACTTTAAAGCGAATGAATATGAAAAAATTAAGCTATTAAAAAACCAAAATTTCACTGAAAGTAATAAATTAATTGCAACAACTAATGAGTTAAATGAACTTACTAGTCAGCTAGATAATATCTTGGTTAGGATTATCAACAAACCACTAGCAAAGTTAGTTAATGATTTTTTAGATGAACAGATTAAACAGATAGTTAAGCTAGATAAAAACAGTTCTGATTTTCACTCAGAAAGTGATAACCTCCCTTTTTATACCAAACTCTTTAATGATTTTCACTTTGGTGTTGATAAATTAATAAACATTAACATAAAAAACCCTCTTTATAACTGGGTTTATAGCCCCAGTTTTTTAATTAGTGAAAGTGATTTTCGCAAGCTTAATGGTATTAGTGGTATCAATAAAAAGCTTTTGGTTGAAAAACTTAGAATTGAAGACATTGTGTTTACAGATCTAAACAAAAAGTATGAAGTTAATGTCTTGACAGAAAGTCCTATTAAAGCACAAAAAACAGTGTTAACTGTGCGCAATATCCTGATGAACGATTATGTTGATAATGAAAGGATTGAATCATATGTCCAACAAGCTAACTTCTTTTTTACTGAGCACTGTAAAAAGATCGGTAAAGAGATCTTAGAATCACTTAATATTTTTATCTCAAGTAGTTCACTACACCGTCATTTTGGCTTTTTAGCATTTCGCTATTCATTTGGACAAAATGTCTTATCCCATAGCCTTGAAACTGCATTTTTAACTGCCCACTTAGCAGCTTTAATAGAACTTGATAGTGAACTGAGTTTAAAGTGTGGATTGCTCCATGATATTGGTAAATCTAATGATGATAATGGTAAAGAGAGCCATACGATTACAGGCGCTAAACTCGCTGAGCAATTTCAACTACCTGATGACATTAAATACACAATTGCTAACCACCACAATAAACATATTGACAATACCTATTGTCGTTTAACACAAATTGCTGATAAACTATCTGCTGCTAGAATTGGTGCTAGAAGTGATAGTTCGCTTCTTTTTAAACAACTAAAAGATGAGTTGAAAAAGATTGTTGATAAAACTATTAATAATTTTCATACAACGATCTTACTAGGTCAAAGTGGTAGAAGGTTAATGATTTGA", "aa": "MNNNITNSIAQLFFNTSFFAFLFLIIIAFNLCLFAYLYFQYRIYKKNPKKANNFKANEYEKIKLLKNQNFTESNKLIATTNELNELTSQLDNILVRIINKPLAKLVNDFLDEQIKQIVKLDKNSSDFHSESDNLPFYTKLFNDFHFGVDKLININIKNPLYNWVYSPSFLISESDFRKLNGISGINKKLLVEKLRIEDIVFTDLNKKYEVNVLTESPIKAQKTVLTVRNILMNDYVDNERIESYVQQANFFFTEHCKKIGKEILESLNIFISSSSLHRHFGFLAFRYSFGQNVLSHSLETAFLTAHLAALIELDSELSLKCGLLHDIGKSNDDNGKESHTITGAKLAEQFQLPDDIKYTIANHHNKHIDNTYCRLTQIADKLSAARIGARSDSSLLFKQLKDELKKIVDKTINNFHTTILLGQSGRRLMI", "aa_hexdigest": "7aa88a0276c97875906d11a6dc92419e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47376", "query_cov": 1.0, "subject_cov": 0.8884297520661157, "identity": 1.0, "score": 833.0, "evalue": 6.94e-304, "valid": true, "gene": "rny", "product": "Ribonuclease Y", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47376", "cog_id": "COG1418", "cog_category": "JR", "go_ids": [ "GO:0005886", "GO:0003723", "GO:0004521", "GO:0006402" ] }, "pscc": { "uniref50_id": "UniRef50_P47376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47376" ], "product": "Ribonuclease Y" }, "genes": [ "rny" ], "id": "KKNAAMEMLJ_316", "locus": "GCA000027325_00277" }, { "type": "cds", "sequence": "contig_1", "start": 157910, "stop": 158020, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGAAAAGATTAAAGCTGAAATTGCTAAGAATCCAATTACAAATCACTTCCCTATTAAAGTTGTAATTAGATATAACTTTGAACACAGTTTTAACACCAAAAGCTAA", "aa": "MVEKIKAEIAKNPITNHFPIKVVIRYNFEHSFNTKS", "aa_hexdigest": "c84b133a1835ffb48e9ee92a5dc25018", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4244.913700000001, "isoelectric_point": 9.698124122619632 }, "id": "KKNAAMEMLJ_317", "locus": "GCA000027325_00278" }, { "type": "cds", "sequence": "contig_1", "start": 158023, "stop": 158247, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG131", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniParc:UPI000013931D", "UniRef:UniRef100_P47377", "UniRef:UniRef50_P47377", "UniRef:UniRef90_P47377" ], "nt": "ATGCAATACAGTGCTTTAATACCACTTTTTATATTACTAATTAGCTTAGTATTATTTTGTTTCAGCTTTAGAAAAAATCAAAGTGAAAATCAGATAGTGAAAATCTTATTTTTTGCTTATTGCATCGATTTTTTAGCTTTAATTCTTGCTGTAATGTTGCTCACTTTTTTAAGTCATGGGTTGTTAAGTTTAGCGATTTTAATCCCTGTCTTAGTTTTTCAATAA", "aa": "MQYSALIPLFILLISLVLFCFSFRKNQSENQIVKILFFAYCIDFLALILAVMLLTFLSHGLLSLAILIPVLVFQ", "aa_hexdigest": "648b043b78ab5a16d3ba8e9a65220ffd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013931D", "uniref100_id": "UniRef100_P47377", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013931D", "UniRef:UniRef100_P47377" ] }, "ips": { "uniref100_id": "UniRef100_P47377", "uniref90_id": "UniRef90_P47377" }, "psc": { "uniref90_id": "UniRef90_P47377", "product": "Uncharacterized protein MG131", "uniref50_id": "UniRef50_P47377", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47377", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47377" ], "product": "Uncharacterized protein MG131" }, "genes": [], "id": "KKNAAMEMLJ_318", "locus": "GCA000027325_00279" }, { "type": "cds", "sequence": "contig_1", "start": 158323, "stop": 158604, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_237711663.1", "SO:0001217", "UniParc:UPI00016400D0", "UniRef:UniRef100_UPI00016400D0", "UniRef:UniRef50_UPI00027B3546", "UniRef:UniRef90_UPI00027B3546" ], "nt": "ATGGAGTTGAAAACCCCTAACTTTAAGCTAATTGATGAAAAGATTGCTGAATTTAATAAGAGTAATGAAAACCTGATTGTAAAACTACTTCAAAAAGAAAAGGAATTTGCCACAAACCAAGTTACTGTTCAGTTTGATACTCAGTCAAAAAAGTCAGAAGAAGTGAAAAAACCTAGTAAAAAAATACTGAAAAGTTATCACTTTCTAACAGTAGTTATGGCAGTTAATTTGCTAATAACTGTTGGTTTGATAGTTGGCTTAATTTTTGCTTTCAGATATTAG", "aa": "MELKTPNFKLIDEKIAEFNKSNENLIVKLLQKEKEFATNQVTVQFDTQSKKSEEVKKPSKKILKSYHFLTVVMAVNLLITVGLIVGLIFAFRY", "aa_hexdigest": "6c0b7f071c36425f583c689b3dcccc55", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00016400D0", "ncbi_nrp_id": "WP_237711663.1", "uniref100_id": "UniRef100_UPI00016400D0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00016400D0", "RefSeq:WP_237711663.1", "UniRef:UniRef100_UPI00016400D0" ] }, "ips": { "uniref100_id": "UniRef100_UPI00016400D0", "uniref90_id": "UniRef90_UPI00027B3546" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3546", "uniref50_id": "UniRef50_UPI00027B3546" }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3546", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3546" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10748.596999999994, "isoelectric_point": 9.52289905548096 }, "id": "KKNAAMEMLJ_319", "locus": "GCA000027325_00280" }, { "type": "cds", "sequence": "contig_1", "start": 158581, "stop": 159006, "strand": "-", "frame": 3, "gene": "hinT", "product": "putative HIT-like protein MG132", "db_xrefs": [ "COG:COG0537", "COG:FR", "GO:0003824", "GO:0009117", "RefSeq:WP_009885688.1", "SO:0001217", "UniParc:UPI000013B27F", "UniRef:UniRef100_P47378", "UniRef:UniRef50_P47378", "UniRef:UniRef90_P47378" ], "nt": "ATGGAAAAAAACACCACAAGTAGTTGTATCTTTTGTGATATTGTTCAAGGTTCAATTACATCCTATAAAATTGGAGAAAATGAGCATGCCATTGCTTTTTTAGATGCTTTTCCTGTAGCTGATGGTCATACTTTAGTAATCCCTAAAAAACATGCAGTTGATTTTTCTTCAACTGATCAAAAAGAGTTACAAGCAGTTAGTTTATTAGCAAAACAAATCGCTTTAAAACTAAAGATGACACTAAAACCATCTGGTTTAAACTATGTTTCTAATGAAGGGGCAATTGCAGGTCAAGTGGTTTTTCATTTTCACTTACACATAGTACCCAAATATGAAACTGGTAAAGGGTTTGGCTATAATGTCAATAAAACCAACAAACGTTCCTTAGAAGAAAACTACCAACTAATATCTGAAAGCAAAAATTAA", "aa": "MEKNTTSSCIFCDIVQGSITSYKIGENEHAIAFLDAFPVADGHTLVIPKKHAVDFSSTDQKELQAVSLLAKQIALKLKMTLKPSGLNYVSNEGAIAGQVVFHFHLHIVPKYETGKGFGYNVNKTNKRSLEENYQLISESKN", "aa_hexdigest": "0b1ad8ce77d4ad4a7658e9422f306df2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013B27F", "ncbi_nrp_id": "WP_009885688.1", "uniref100_id": "UniRef100_P47378", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013B27F", "RefSeq:WP_009885688.1", "UniRef:UniRef100_P47378" ] }, "ips": { "uniref100_id": "UniRef100_P47378", "uniref90_id": "UniRef90_P47378" }, "psc": { "uniref90_id": "UniRef90_P47378", "gene": "HinT", "product": "Uncharacterized HIT-like protein MG132", "uniref50_id": "UniRef50_P47378", "cog_id": "COG0537", "cog_category": "FR", "go_ids": [ "GO:0003824", "GO:0009117" ] }, "pscc": { "uniref50_id": "UniRef50_P47378", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47378" ], "product": "Uncharacterized HIT-like protein MG132" }, "genes": [ "hinT" ], "id": "KKNAAMEMLJ_320", "locus": "GCA000027325_00281" }, { "type": "cds", "sequence": "contig_1", "start": 159554, "stop": 159670, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGATTACTGCACTGATTAGTGTTGCTAACAGTGGTCAAATGAGCCTGATTTGGTTTACTAATTTTGATACTTTTACTTACCAAAGTAATAGCCTTGCAATCTTTTTAGTATGA", "aa": "MVITALISVANSGQMSLIWFTNFDTFTYQSNSLAIFLV", "aa_hexdigest": "25df52711d8cc99d50c5732025be87d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4244.880099999999, "isoelectric_point": 4.0500284194946286 }, "id": "KKNAAMEMLJ_321", "locus": "GCA000027325_00282" }, { "type": "cds", "sequence": "contig_1", "start": 159798, "stop": 160100, "strand": "+", "frame": 3, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_010869347.1", "SO:0001217", "UniParc:UPI000013932E", "UniRef:UniRef100_P47380", "UniRef:UniRef50_P47380", "UniRef:UniRef90_P47380" ], "nt": "ATGAGTTTTAAAAAGATTGCTGAAATGATGCGTCAAGCAGAACGAGAAACTAAGAAAAAAACATTAGCGTTTGAACAACAAGCCTTTGAATACAACTATAAAAATGGTGCGATTAAGATCACTATTTTAGGTGATCTTACACTTAAATCAATTAACATCGATCCTGTTTTGATTGATGCAAGTGACAAAGTTATTCTAGAGGAGATGATTATAGAAGCTACTAATGAAGCGGTTAGTGATGTGAAAACCAAGTATGATAACTTAGTTGAGAAAACTATGCCAAAAGTTCCAGGTCTTTTCTAA", "aa": "MSFKKIAEMMRQAERETKKKTLAFEQQAFEYNYKNGAIKITILGDLTLKSINIDPVLIDASDKVILEEMIIEATNEAVSDVKTKYDNLVEKTMPKVPGLF", "aa_hexdigest": "c413e76280bd3195594ee858ba01ca99", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013932E", "ncbi_nrp_id": "WP_010869347.1", "uniref100_id": "UniRef100_P47380", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013932E", "RefSeq:WP_010869347.1", "UniRef:UniRef100_P47380" ] }, "ips": { "uniref100_id": "UniRef100_P47380", "uniref90_id": "UniRef90_P47380" }, "psc": { "uniref90_id": "UniRef90_P47380", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_P47380", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_P47380", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47380" ], "product": "Uncharacterized protein MG134" }, "genes": [ "ybaB" ], "id": "KKNAAMEMLJ_322", "locus": "GCA000027325_00283" }, { "type": "cds", "sequence": "contig_1", "start": 160072, "stop": 160263, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGACCTTTCTTAACTTAAGCAATAGTGATCCACAACTTGTGTTTATTGTGGGCTTTAGTTTAAGTTATGTTGCAATTTTACTAATGACACTCTTTGCTAATATCCCTACATTTATGGAAATGAAAAAGGATAAGATCTTTATTAAAAAAGAAAATCTAATTAGAAAAGACCTGGAACTTTTGGCATAG", "aa": "MATFLNLSNSDPQLVFIVGFSLSYVAILLMTLFANIPTFMEMKKDKIFIKKENLIRKDLELLA", "aa_hexdigest": "a115bad6ad2a63a293ff095ede57b6f9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7239.664999999997, "isoelectric_point": 8.064559745788571 }, "id": "KKNAAMEMLJ_323", "locus": "GCA000027325_00284" }, { "type": "cds", "sequence": "contig_1", "start": 160285, "stop": 160914, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG135", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47381", "UniRef:UniRef90_P47381" ], "nt": "ATGCAGACACTTAACTACCTTGTCATCATCCTTATTATTGCTGGTGTAATTTCTGTTTTAGCTTTTACTCCTTTAGTTCGAAAACTAAAGATTAGGTTCTATCTAATTCAAGTACTAGCCATTGTACTGTTTGTCTATGTTTTTTTTGGTAGGCAGATTATTTATTTATTCCCTGATGTTTATGGACAAAATTCCCAATCTTCACAAAATTTAGATTCACTACGACTAAGTAGGATATTTTTATTAGATCTTTGTCCATTTTTTGCTGTTATTGCGCCAGTGTTTGTCTTTTTAAAGCAAAAAAAGATTAGTGGGGTGTTAGCTGTTTTTGGGTTATTTGGAGCTTTGGTTACTCTTTTTGGTGAACTAATTTTTACACCTGTTAATGAACAAGATATTGTGAATTTCATCTTTGTTGGTACAGGAAATAACCAGATTTATTTCATGATGCATTTTCTCAGTTTACTTGTAAGCTTAGCAATTATCTTGTGGGATAACTGTTTTTCTTTAATCTCATTTTTCTATATCCATGTGTTTGCTCTAATTTACTTTAGTTATGTAGCTTTGATGGTATCTGTGTTTAAGGGCCAGATCACAGGTAATACAACTGGTATCTTAGCAAGTGATTGA", "aa": "MQTLNYLVIILIIAGVISVLAFTPLVRKLKIRFYLIQVLAIVLFVYVFFGRQIIYLFPDVYGQNSQSSQNLDSLRLSRIFLLDLCPFFAVIAPVFVFLKQKKISGVLAVFGLFGALVTLFGELIFTPVNEQDIVNFIFVGTGNNQIYFMMHFLSLLVSLAIILWDNCFSLISFFYIHVFALIYFSYVALMVSVFKGQITGNTTGILASD", "aa_hexdigest": "6afb3412eeae92f343019960577df7d4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 160072, "stop": 160914, "observations": { "mutation": [ 160287 ], "stop": [ 160287 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47381", "query_cov": 1.0, "subject_cov": 0.7464285714285714, "identity": 1.0, "score": 383.0, "evalue": 6.29e-133, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MQTLNYLVIILIIAGVISVLAFTPLVRKLKIRFYLIQVLAIVLFVYVFFGRQIIYLFPDVYGQNSQSSQNLDSLRLSRIFLLDLCPFFAVIAPVFVFLKQKKISGVLAVFGLFGALVTLFGELIFTPVNEQDIVNFIFVGTGNNQIYFMMHFLSLLVSLAIILWDNCFSLISFFYIHVFALIYFSYVALMVSVFKGQITGNTTGILASDWTNGEYKNVATFLNLSNSDPQLVFIVGFSLSYVAILLMTLFANIPTFMEMKKDKIFIKKENLIRKDLELLA" }, "paralog": false, "identity": 0.9964285714285714, "score": 537.0, "evalue": 2.49e-196, "description": "Internal stop codon at 160287. Nonsense mutation around 160287.", "psc": { "uniref90_id": "UniRef90_P47381", "product": "Uncharacterized protein MG135", "uniref50_id": "UniRef50_P47381", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47381", "UniRef:UniRef50_P47381", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47381", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47381" ], "product": "Uncharacterized protein MG135" } }, "id": "KKNAAMEMLJ_324", "locus": "GCA000027325_00285" }, { "type": "cds", "sequence": "contig_1", "start": 160963, "stop": 161391, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAGTGATCGTTTAAATGATCAAGCCCAACATCGCTTGCAGAAACTTTTAAGGTTAAAACAAACTAATAATGACCCTTATTTAGTAACAAAAACTAGTCTAACCCATTCTTCAAAAAGCTTTCAAGTTGAATTTGAAAAATGTTCAGAAGAAGAGTTGAAGAAAAAAGCAACTGTCTCACTAGCTGGAAGGATCATTGCTATTAGACAAACCTTTTTAATTATTCAAGATTTTGATGGTCAAGTCCAACTTTACATCAATAAAAAAATCCATCCTAAGTTATTTGATTACTTTAATGAATTTGTTGATATTGGTGATCAAATTGTTGTTAGTGGTAAGCCAATGTTAACTAAAACAAAGGTATTAACTTTAGCTGTTGAAGAGATGAAAATCATTGCTAAGTGTTTATTGGTTCCACCTGAAAAGTGA", "aa": "MSDRLNDQAQHRLQKLLRLKQTNNDPYLVTKTSLTHSSKSFQVEFEKCSEEELKKKATVSLAGRIIAIRQTFLIIQDFDGQVQLYINKKIHPKLFDYFNEFVDIGDQIVVSGKPMLTKTKVLTLAVEEMKIIAKCLLVPPEK", "aa_hexdigest": "5f3f6cc84b125ee95b1304f6806373ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 79, "aa_cov": 0.5211267605633803, "hmm_cov": 0.9493670886075949, "evalue": 2.0187705837423645e-13, "score": 45.21485900878906, "start": 59, "stop": 132 } ], "seq_stats": { "molecular_weight": 16349.955899999994, "isoelectric_point": 9.205779075622559 }, "id": "KKNAAMEMLJ_325", "locus": "GCA000027325_00286" }, { "type": "cds", "sequence": "contig_1", "start": 161458, "stop": 161856, "strand": "+", "frame": 1, "gene": null, "product": "Lysine--tRNA ligase, heat inducible", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X2LQM9" ], "nt": "ATGCGTGATGTTTTTCTGAAACGCACTAAGATTATTAAATCAATCCGTAGCTTTCTTGATCAAAATGGTTTTATTGAAGTTGAAACCCCCACTTTACAAGCTGTTTTAGGAGGAGCTAATGCTAAACCCTTTAAAACCCATTACAATGCTTTAAAAGCGGATTTTTATCTCAGAATTGCTAATGAAATAGCATTAAAAAAACTCATTATTGGTGGATTTAACAAGGTTTATGAAATGGGTAAAATGTTCCGTAATGAAGGGGTTGATACTACCCACAATCCTGAGTTTACCAGTATTGAAATATATCAAGCTTATGCAGATTTTGAAGTCATGCTTGTGCTTGTTGAAAAGCTGATTCAATCACTTTGTGAAAGCTTAAACCAATTTAGCTTTAACTGA", "aa": "MRDVFLKRTKIIKSIRSFLDQNGFIEVETPTLQAVLGGANAKPFKTHYNALKADFYLRIANEIALKKLIIGGFNKVYEMGKMFRNEGVDTTHNPEFTSIEIYQAYADFEVMLVLVEKLIQSLCESLNQFSFN", "aa_hexdigest": "7e3ef15d9340f4a29c6ffafa1d3c30b7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X2LQM9", "query_cov": 0.9242424242424242, "subject_cov": 0.8652482269503546, "identity": 0.5489999999999999, "score": 149.0, "evalue": 6.66e-44, "valid": false, "product": "Lysine--tRNA ligase, heat inducible", "uniref50_id": "UniRef50_A0A2X2LQM9" }, "pscc": { "uniref50_id": "UniRef50_A0A2X2LQM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X2LQM9" ], "product": "Lysine--tRNA ligase, heat inducible" }, "genes": [], "id": "KKNAAMEMLJ_326", "locus": "GCA000027325_00287" }, { "type": "cds", "sequence": "contig_1", "start": 161902, "stop": 162435, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGTTGAACTTATTAAGAAAGTTACAGGGATCGATTTTAATTCAGTAAAAGATGATCAATCTGCCATTTTATTAGCAGAAAAACATCATGTTAAACTAGCAAAACACCAACAAAATAAGCAACACATCATTAATTTGTTTTTTGAACAGTTTTGTGAACAAACATTAATTGAACCTACCTTTGTAACCCATTATCCAAAAGCAGTTTCTCCTTTAGCAAAACAAGATCCTTCAAATCCTGAATTCACCCAACGATTTGAACTTTTTATTAATGGTAAAGAGATTGCTAATGCTTACAGTGAGCTAAACGATCCTTTAGAACAAAGAAAAAGGTTTGAACAACAACTTGAAGAAAAACAGCTTGGTAATGATGAGACAAGTGAACTTGATGAATCGTTTTTAGAAGCATTAAGTTTTGGGATGGTAAACACTGCTGGGCTTGGGATAGGTATTGATCGTTTGGTAATGTTGTTATGTGAATGTAATTCTATCCGTGATGTTGTTTTCTTCCCCCAGTTGCGTGAACATAAATAG", "aa": "MVELIKKVTGIDFNSVKDDQSAILLAEKHHVKLAKHQQNKQHIINLFFEQFCEQTLIEPTFVTHYPKAVSPLAKQDPSNPEFTQRFELFINGKEIANAYSELNDPLEQRKRFEQQLEEKQLGNDETSELDESFLEALSFGMVNTAGLGIGIDRLVMLLCECNSIRDVVFFPQLREHK", "aa_hexdigest": "e535823dc2549131097509eb98553db5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031D0E1C7", "query_cov": 0.9830508474576272, "subject_cov": 0.8093023255813954, "identity": 0.546, "score": 182.0, "evalue": 2.26e-55, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A349PV97", "kegg_orthology_id": "K04567" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 127, "aa_cov": 0.6892655367231638, "hmm_cov": 0.968503937007874, "evalue": 1.4483350441546025e-37, "score": 124.41230773925781, "start": 52, "stop": 173 } ], "seq_stats": { "molecular_weight": 20391.9711, "isoelectric_point": 5.15656986236572 }, "id": "KKNAAMEMLJ_327", "locus": "GCA000027325_00288" }, { "type": "cds", "sequence": "contig_1", "start": 162422, "stop": 163237, "strand": "+", "frame": 2, "gene": "glf", "product": "UDP-galactopyranose mutase", "db_xrefs": [ "COG:COG0562", "COG:M", "EC:5.4.99.9", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660", "KEGG:K01854", "SO:0001217", "UniRef:UniRef50_P75499", "UniRef:UniRef90_Q49398" ], "nt": "GTGAACATAAATAGTTTTGATATTTTAATTGTTGGTGCTGGTATTAGTGGAATAGTACTAGCTAACATCTTAGCTAATCACAATAAAAGGGTTTTAATTGTTGAAAAAAGAGATCATATTGGTGGTAACTGTTATGATAAAGTTGATAGTAAAACTCAACTCTTGTTTCACCAGTATGGACCCCATATTTTCCATACTAACAACCAAACTGTTATTAACTTTATCTCACCCTTCTTTGAACTAAATAACTACCACCATCGGGTTGGTTTAAAATTGAAAAATAACCTAGATTTAACCTTACCCTTTGATTTTCAACAGATCTATAAACTAATGGGAAAAGATGGTAGAAAACTCGTTAGTTTTTTTAAAGAAAATTTCAGTTTAAATACTCATCTATCATTAGCAGAATTACAACTAATTGATAATCCTTTAGCACAAAAACTCTATCAGTTTTTAATTAGTAATGTTTATAAACCATACAGTGTCAAAATGTGGGGTTTACCATTTGCAATGATTAATGAGAATGTTATTAACAGGGTCAAGATAGTTTTAAGTGAACAAAGCAGTTATTTTCCTGATGCAATTATCCAGGGATTACCTAAATCAGGTTATACAAACAGTTTTCTTAAGATGTTAGCCAATCCCTTAATTGATGTGCAGTTAAACTGCAAAGATAACCTTTTAGTTTATCAAGATGAAAAACTGTTTTTTAACAATAACTTAATAGAAAAACCAGTTGTTTACTGTGGCTTAATTGACAAGCTATTTAACTTTTGCTTTGGTCATTTGCAATACCGTTCTCTTGCCTTTAGTTGA", "aa": "MNINSFDILIVGAGISGIVLANILANHNKRVLIVEKRDHIGGNCYDKVDSKTQLLFHQYGPHIFHTNNQTVINFISPFFELNNYHHRVGLKLKNNLDLTLPFDFQQIYKLMGKDGRKLVSFFKENFSLNTHLSLAELQLIDNPLAQKLYQFLISNVYKPYSVKMWGLPFAMINENVINRVKIVLSEQSSYFPDAIIQGLPKSGYTNSFLKMLANPLIDVQLNCKDNLLVYQDEKLFFNNNLIEKPVVYCGLIDKLFNFCFGHLQYRSLAFS", "aa_hexdigest": "caac98b92d30296bd5c84c16b991208b", "start_type": "GTG", "rbs_motif": null, "genes": [ "glf" ], "truncated": "3-prime", "pseudogene": { "start": 162377, "stop": 163540, "observations": { "mutation": [ 163235, 163469 ], "stop": [ 163235, 163469 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49398", "query_cov": 1.0, "subject_cov": 0.6707920792079208, "identity": 0.996, "score": 548.0, "evalue": 4.34e-195, "gene_start": 1, "gene_end": 271, "reference_start": 16, "reference_end": 286, "reference_sequence": "MNVILSVMLFSSPSCVNINSFDILIVGAGISGIVLANILANHNKRVLIVEKRDHIGGNCYDKVDSKTQLLFHQYGPHIFHTNNQTVINFISPFFELNNYHHRVGLKLKNNLDLTLPFDFQQIYKLMGKDGRKLVSFFKENFSLNTHLSLAELQLIDNPLAQKLYQFLISNVYKPYSVKMWGLPFAMINENVINRVKIVLSEQSSYFPDAIIQGLPKSGYTNSFLKMLANPLIDVQLNCKDNLLVYQDEKLFFNNNLIEKPVVYCGLIDKLFNFCFGHLQYRSLAFSWKRFNQKKYQTYPVVNMPLAKSITRSVEYKQLTNQGSFKPQTIVSFETPGSYAINDPRFNEPYYPINNTLNDTLFKKYWKKASKLKNLHLLGRLATYQYIDMDKAILLSIKKAQQLLS" }, "paralog": false, "identity": 0.9922480620155039, "score": 777.0, "evalue": 5.41e-288, "description": "Internal stop codon at 163235, 163469. Nonsense mutation around 163235, 163469.", "psc": { "uniref90_id": "UniRef90_Q49398", "gene": "glf", "product": "UDP-galactopyranose mutase", "ec_ids": [ "5.4.99.9" ], "uniref50_id": "UniRef50_P75499", "cog_id": "COG0562", "cog_category": "M", "kegg_orthology_id": "K01854", "go_ids": [ "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49398", "EC:5.4.99.9", "UniRef:UniRef50_P75499", "COG:COG0562", "COG:M", "KEGG:K01854", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75499" ], "product": "UDP-galactopyranose mutase" } }, "id": "KKNAAMEMLJ_328", "locus": "GCA000027325_00289" }, { "type": "cds", "sequence": "contig_1", "start": 163283, "stop": 163471, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTAGCTAAATCAATCACAAGGAGTGTGGAATACAAACAACTAACAAACCAAGGTTCTTTCAAACCGCAAACCATCGTTAGTTTTGAAACCCCTGGCAGCTATGCAATTAACGATCCTAGGTTTAATGAACCTTATTACCCAATTAACAATACACTAAATGATACTCTTTTTAAAAAGTACTGA", "aa": "MPLAKSITRSVEYKQLTNQGSFKPQTIVSFETPGSYAINDPRFNEPYYPINNTLNDTLFKKY", "aa_hexdigest": "6b8a697082f073d04bad490dc692dfc9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7178.007700000001, "isoelectric_point": 9.04899196624756 }, "id": "KKNAAMEMLJ_329", "locus": "GCA000027325_00290" }, { "type": "cds", "sequence": "contig_1", "start": 163591, "stop": 165387, "strand": "+", "frame": 1, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "COG:COG0481", "COG:J", "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "RefSeq:WP_010869350.1", "SO:0001217", "UniParc:UPI000012E47A", "UniRef:UniRef100_P47384", "UniRef:UniRef50_B1X3K4", "UniRef:UniRef90_P47384" ], "nt": "ATGGAACAAAAAAACATTAGAAATTTTTCTATTATTGCCCATATTGATCATGGTAAATCTACCTTATCAGACCGCTTGTTAGAACATAGTTTAGGCTTTGAAAAAAGACTATTACAAGCGCAAATGCTTGATACTATGGAGATTGAAAGAGAAAGGGGTATTACCATTAAATTAAATGCTGTTGAATTGAAAATTAATGTTGATAACAACAACTATCTTTTTCATTTAATTGACACCCCTGGGCATGTTGATTTTACTTATGAAGTGTCTCGTTCTTTAGCAGCTTGTGAGGGAGTTTTATTGTTAGTAGATGCAACCCAAGGAATTCAAGCACAAACGATTTCCAATGCTTATCTTGCGTTGGAAAATAACCTGGAAATTATCCCAGTTATTAACAAGATAGATATGGATAATGCTGATATTGAAACAACAAAAGATTCACTCCATAACTTATTAGGAGTTGAAAAGAACAGTATCTGTTTAGTATCTGCAAAAGCTAACTTAGGGATTGATCAGTTAATTCAAACAATTATAGCTAAGATCCCCCCACCAAAAGGAGAAATTAATAGACCTTTAAAAGCATTACTCTTTGATAGTTACTATGATCCTTACAAGGGGGTTGTTTGTTTTATTAGGGTATTTGATGGTTGTTTAAAGGTTAATGATAAGGTTCGTTTTATTAAAAGTAATTCTGTTTACCAAATTGTGGAACTAGGGGTTAAAACCCCATTTTTTGAAAAAAGAGATCAATTGCAAGCAGGAGATGTTGGTTGGTTTTCAGCAGGGATAAAAAAACTTCGTGATGTTGGGGTTGGTGATACTATTGTTAGTTTTGATGATCAATTTACAAAACCCCTAGCAGGTTATAAAAAGATCTTACCCATGATCTATTGTGGTTTATATCCAGTTGATAACAGTGATTATCAAAACCTCAAGTTAGCGATGGAAAAGATCATAATCAGTGATGCAGCATTGGAATATGAATATGAAACATCCCAAGCGTTAGGTTTTGGGGTTAGGTGTGGTTTTCTAGGTCTTTTACATATGGATGTTATTAAAGAAAGATTGGAAAGAGAATACAACCTAAAACTCATCTCAGCTCCCCCTTCAGTTGTATATAAGGTGTTGTTAACAAATGGTAAAGAGATTAGTATTGACAATCCCTCTTTGTTACCAGAACGCTCCAAGATTAAAGCAATCAGTGAACCATTTGTAAAAGTCTTTATTGATTTACCTGATCAATATTTGGGCAGTGTTATTGATTTATGCCAAAACTTCAGGGGTCAATATGAAAGTTTAAATGAGATTGATATCAACAGAAAAAGAATCTGTTATCTGATGCCTTTAGGGGAAATTATCTACAGTTTTTTTGATAAGTTAAAGTCGATTAGTAAGGGTTATGCATCGTTAAACTATGAGTTTTATAACTACCAACATAGTCAACTGGAAAAAGTTGAGATCATGTTAAACAAACAAAAGATTGATGCATTATCTTTTATCAGTCATAAAGACTTTGCTTTTAAGCGGGCAAAAAAGTTTTGCACTAAGCTCAAAGAATTGATTCCCAAGCATCTGTTTGAGATCCCTATCCAAGCAACAATAGGGAGTAAAGTAATAGCAAGAGAGACAATCAAAGCAGTTAGAAAGGATGTAATAGCTAAACTTTATGGAGGGGATGTTAGTAGAAAAAAGAAGTTATTAGAGAAGCAAAAAGAGGGTAAAAAACGCTTGAAAGCAGTTGGGAGTGTTCAATTACCCCAAGAGCTATTTAGTCATTTGCTGAAAGATGAAGATTAA", "aa": "MEQKNIRNFSIIAHIDHGKSTLSDRLLEHSLGFEKRLLQAQMLDTMEIERERGITIKLNAVELKINVDNNNYLFHLIDTPGHVDFTYEVSRSLAACEGVLLLVDATQGIQAQTISNAYLALENNLEIIPVINKIDMDNADIETTKDSLHNLLGVEKNSICLVSAKANLGIDQLIQTIIAKIPPPKGEINRPLKALLFDSYYDPYKGVVCFIRVFDGCLKVNDKVRFIKSNSVYQIVELGVKTPFFEKRDQLQAGDVGWFSAGIKKLRDVGVGDTIVSFDDQFTKPLAGYKKILPMIYCGLYPVDNSDYQNLKLAMEKIIISDAALEYEYETSQALGFGVRCGFLGLLHMDVIKERLEREYNLKLISAPPSVVYKVLLTNGKEISIDNPSLLPERSKIKAISEPFVKVFIDLPDQYLGSVIDLCQNFRGQYESLNEIDINRKRICYLMPLGEIIYSFFDKLKSISKGYASLNYEFYNYQHSQLEKVEIMLNKQKIDALSFISHKDFAFKRAKKFCTKLKELIPKHLFEIPIQATIGSKVIARETIKAVRKDVIAKLYGGDVSRKKKLLEKQKEGKKRLKAVGSVQLPQELFSHLLKDED", "aa_hexdigest": "d202830bdfce797022ecf07c0f2ef086", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E47A", "ncbi_nrp_id": "WP_010869350.1", "uniref100_id": "UniRef100_P47384", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E47A", "RefSeq:WP_010869350.1", "UniRef:UniRef100_P47384" ] }, "ips": { "uniref100_id": "UniRef100_P47384", "uniref90_id": "UniRef90_P47384" }, "psc": { "uniref90_id": "UniRef90_P47384", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_B1X3K4", "cog_id": "COG0481", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "genes": [ "lepA" ], "id": "KKNAAMEMLJ_330", "locus": "GCA000027325_00291" }, { "type": "cds", "sequence": "contig_1", "start": 165471, "stop": 167129, "strand": "+", "frame": 3, "gene": "rnj", "product": "Ribonuclease J", "db_xrefs": [ "COG:COG0595", "COG:J", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0004534", "GO:0005737", "GO:0006364", "GO:0008270", "SO:0001217", "UniRef:UniRef50_P75497", "UniRef:UniRef90_P47385" ], "nt": "ATGATAAAGGATTTTAATCCTGGTGATTTTATTGGTAAAAAACCAACTAAAATCTATGCTTTTGGTGGTATCCAAGAAGTTGGTAAAAACATGTATGGGATTGAATATGATGATGAAATCATCATTATTGACTGTGGCATTAAATTTGCTAGTGATGATCTACTTGGCATCAATGGGATTATCCCTAGTTTTGAACACTTAATTGAAAACCAAAGTAAGGTTAAAGCATTGTTTATTACCCATGGTCATGAAGACCATATTGGGGGTGTACCATACCTTTTAAAGCAGGTTGATATTCCTGTTATCTACGCACCAAGGATCGCAGCATCATTAATCTTGAAAAAGGTTAATGAGCACAAGGATGCTAAGCTCAATAAGATAGTTACTTTTGATGATTTTAGTGAGTTTCAAACCAAACACTTCAAAATTGATTTTTACCGGGTAAACCACTCGATTCCCGATGCTTTTGGAATCTGTGTGCAAACCCCTAATGGCAACATTGTTCAAAGCGGTGACTACCGGTTTGATTTTGCTGCTGGGAGTGAGATGTTAGATGTTCATAAAGTAGTGAAAATTGCCGAGCGCAATGTCCATGTTTTTATGAGTGAATCTACTAATGCTGAAGTACCAGGTTTTTCCCAAAGTGAAAAGTTAATTTACAGAAACATCCAAAAGATCTTAAAAGAAGCAAGGGGTAGGGTTATTTTAACTACTTTTGCATCTAACATCACACGGATTAATGAAATTATTGAGATTGCTTTAAACAACAAACGCAAGATCTGTTTATTGGGTAAATCAATGGATGTTAATGTTAATATTTCACGCAAAATTGGATTGATGGCAATTGATAGTAATGATATTGTGGAAGTTCGTGATATCAAAAACTATCCTGATCGTAATATCTTAATCTTGTGCACTGGTTCACAAGGTGAGGAGGCTGCTGCTTTAAACACAATGGCACGTGGTAAGCATAATTGGGTGAGCTTAAAATCAACTGACACCATTATTATGTCTTCAAATCCAATTCCAGGTAATTATGCTGCAGTTGAAAACTTGCTTAATGAACTCTCTAAGTTTGGTGTTGCTATTTATGAAAATTCATCCCAACTAAAACTACATGCCTCAGGTCATGCCACTCAACAAGAGTTACAGTTGATGCTAAATTTAATGTTTCCTAAATACTTAATTCCTATCCATGGTGAATTTAAGATGATGCGAACCATAAAAAACATTGCTAATGAATGTGGCATTAAAAGCGAGGATGTGGCGCTTTTAAGTAATGGCCAAGTAATGTATTTAATTGATGAAGAACTTTATTATTCCAATGAAATTATTAATGCTGATCCTATTTATATAGAGAGTCATAACTCTTCTCCTGATCTTGCAAGAATAATTAAGCAAAGACAAATCCTTAGTCGTGATGGGATGTTTGCTGTTATTGTTGTTTTTGATAAGAATAATAACATCATTGGGATTCCAACCTTAATAACAAGGGGTTGTTTCTTTGCACTTGATTCCAATCCTTTAATGACAAAGATAGCCCATTCTGTTAAAAGAACTTTAGAAAGTGTTATCCAAAGTAAGAAGTTTAATAGTCATGAACAACTAACAAAGGAATTGAAACGAGTTTGTAAGGAAACTGTTTCTTACTTTATCTGA", "aa": "MIKDFNPGDFIGKKPTKIYAFGGIQEVGKNMYGIEYDDEIIIIDCGIKFASDDLLGINGIIPSFEHLIENQSKVKALFITHGHEDHIGGVPYLLKQVDIPVIYAPRIAASLILKKVNEHKDAKLNKIVTFDDFSEFQTKHFKIDFYRVNHSIPDAFGICVQTPNGNIVQSGDYRFDFAAGSEMLDVHKVVKIAERNVHVFMSESTNAEVPGFSQSEKLIYRNIQKILKEARGRVILTTFASNITRINEIIEIALNNKRKICLLGKSMDVNVNISRKIGLMAIDSNDIVEVRDIKNYPDRNILILCTGSQGEEAAALNTMARGKHNWVSLKSTDTIIMSSNPIPGNYAAVENLLNELSKFGVAIYENSSQLKLHASGHATQQELQLMLNLMFPKYLIPIHGEFKMMRTIKNIANECGIKSEDVALLSNGQVMYLIDEELYYSNEIINADPIYIESHNSSPDLARIIKQRQILSRDGMFAVIVVFDKNNNIIGIPTLITRGCFFALDSNPLMTKIAHSVKRTLESVIQSKKFNSHEQLTKELKRVCKETVSYFI", "aa_hexdigest": "ce85e26158e04109b5549b8cf0c48792", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47385", "query_cov": 1.0, "subject_cov": 0.9701230228471002, "identity": 1.0, "score": 1091.0, "evalue": 0.0, "valid": true, "gene": "rnj", "product": "Ribonuclease J", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75497", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0004534", "GO:0003723", "GO:0004521", "GO:0008270", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_P75497", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75497" ], "product": "Ribonuclease J" }, "genes": [ "rnj" ], "id": "KKNAAMEMLJ_331", "locus": "GCA000027325_00292" }, { "type": "cds", "sequence": "contig_1", "start": 167209, "stop": 167556, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAAAAGTGGTATTAGAGTACCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACCCAATCATCCTTCAACCCCACCCAAAGGTTGAAGAGTGGGAGTCCAATGAAGGATACAGGAAAGATGGGGGAGAAACTCAGTGAAACAACTGCTTCATCCATGAGTGGTATGGCTACATCCACTCGAGCCAAGGCCCTTAAGATAGAGGTGGAAAGGGGGAGTAATGTCAATCAAGGCGAACTACAATCCAACGACTTTGCCAAAAAGCCGTTTAAAGATGAGAGCAATAAGAAGTTGGATTCACAGAAGGAGTTTCCCCAAGGAAAGGTTTGA", "aa": "MPKVVLEYHNLNNQVVKESLEVEATQSSFNPTQRLKSGSPMKDTGKMGEKLSETTASSMSGMATSTRAKALKIEVERGSNVNQGELQSNDFAKKPFKDESNKKLDSQKEFPQGKV", "aa_hexdigest": "b1c2a07fc6c7d04004b603c30b07e667", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D4FD3B", "query_cov": 1.0, "subject_cov": 0.8984375, "identity": 0.7390000000000001, "score": 129.0, "evalue": 1.32e-36, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12694.151799999985, "isoelectric_point": 9.155816078186035 }, "id": "KKNAAMEMLJ_332", "locus": "GCA000027325_00293" }, { "type": "cds", "sequence": "contig_1", "start": 167495, "stop": 167737, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGGGGGGTTGAGGAAGCAGAAGTTGAAGGGGAGGGGGAGTTGGAGAAGGGTTTACCATTGCCGATTCAGGGGAGAAAGTCTAAGTCGCCCCCATCCCCCTCTCTCTTGTTATCTCATCTGTACTCAACACCGGTTTTCAAACCTTTCCTTGGGGAAACTCCTTCTGTGAATCCAACTTCTTATTGCTCTCATCTTTAA", "aa": "MIIDLTPTLMLEKVGRGVEEAEVEGEGELEKGLPLPIQGRKSKSPPSPSLLLSHLYSTPVFKPFLGETPSVNPTSYCSHL", "aa_hexdigest": "b76c811aa83009a19664f00a42c3a161", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8674.924099999997, "isoelectric_point": 5.243419837951659 }, "id": "KKNAAMEMLJ_333", "locus": "GCA000027325_00294" }, { "type": "cds", "sequence": "contig_1", "start": 167859, "stop": 168230, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030CFBCE2" ], "nt": "GTGAAACCTAGCAATAGTAGTACCCCCTTTAATCCCACCTCAGCTGGTGGCTCCTCAGCCAAAAAAACAACCACCTATTCCTTTTTACCCAATTCCATCAGTCCCACCAGTGACTGGATCAACGCATTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAACTGTTGTTAAGAGCACTGTTAGGAACTATTCCGGTCTTGATCAATAAGAGTGGGGATAGTAATGATCAATTTAACAAGGATAGTGAGCAGAAATGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTTATAAGATTTTTACTTATTTAATAGTTAAAAAAGTTTTGAATTTTTCTTAG", "aa": "MKPSNSSTPFNPTSAGGSSAKKTTTYSFLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGDSNDQFNKDSEQKWDKTNEKDGNLPGFGEVNGGFYKIFTYLIVKKVLNFS", "aa_hexdigest": "2194e9c61922fc8416e4eb588946e177", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030CFBCE2", "query_cov": 0.983739837398374, "subject_cov": 0.9029850746268657, "identity": 0.8759999999999999, "score": 212.0, "evalue": 3.98e-69, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_UPI0030CFBCE2" }, "pscc": { "uniref50_id": "UniRef50_UPI0030CFBCE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030CFBCE2" ], "product": "adhesin" }, "genes": [], "id": "KKNAAMEMLJ_334", "locus": "GCA000027325_00295" }, { "type": "cds", "sequence": "contig_1", "start": 168634, "stop": 168807, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGAAAAAATATCAGTTAGATGTACCGAATGTTTCAACAACCATGAACGAAGTTTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCCAAAGAGAAGGGTAAGACACAAGTAGCGGTACATCTGGGTAGTGGGCAAGCAAATCAGTGA", "aa": "MKKYQLDVPNVSTTMNEVLQELILEQPLTKYTLNSSLAKEKGKTQVAVHLGSGQANQ", "aa_hexdigest": "2f2ea21062fc4a60f82cd225b046ea13", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 168505, "stop": 168866, "observations": { "deletion": [ 168817 ], "mutation": [ 168625, 168805 ], "stop": [ 168625, 168805, 168829 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 1.0, "subject_cov": 0.47107438016528924, "identity": 0.807, "score": 95.1, "evalue": 4.97e-24, "gene_start": 1, "gene_end": 57, "reference_start": 43, "reference_end": 99, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7520661157024794, "score": 167.0, "evalue": 2.7e-55, "description": "Internal stop codon at 168625, 168805, 168829. Frameshift due to deletion around 168817. Nonsense mutation around 168625, 168805.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6316.175900000002, "isoelectric_point": 8.05663013458252 }, "id": "KKNAAMEMLJ_335", "locus": "GCA000027325_00296" }, { "type": "cds", "sequence": "contig_1", "start": 169330, "stop": 169545, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "TTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACTTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGCTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGA", "aa": "MGYKETTSTNSHNFHSKSFTQLAYLISGIDSVNDQIIFSGFKAGSVGYDSSSSSSSSSSSSSSSTKDQALA", "aa_hexdigest": "553debb1d924d530ade54fe8aa981e1d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 61, "aa_cov": 0.8309859154929577, "hmm_cov": 1.0, "evalue": 1.0647250184557768e-16, "score": 55.99409484863281, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 7398.783599999999, "isoelectric_point": 5.974028205871583 }, "id": "KKNAAMEMLJ_336", "locus": "GCA000027325_00297" }, { "type": "cds", "sequence": "contig_1", "start": 169591, "stop": 169815, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D266AC", "UniRef:UniRef90_UPI00027B3D0D" ], "nt": "GTGACCAACGACACGGGATTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCTCATTCGTTGTTCCTTATTCGGGGAATCATAGTAATCAAATTTCATCAGGAACCATTAAAACTGCTTATCCTGTGAAAAAAGATCAAAAATCAACTGTCAAGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATTTAAACATTAAATAA", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSGNHSNQISSGTIKTAYPVKKDQKSTVKINSLINATPLNSYGDLNIK", "aa_hexdigest": "a9486c0e6c9496c873a96350175ac184", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "3-prime", "pseudogene": { "start": 169621, "stop": 169850, "observations": { "deletion": [ 169807 ], "stop": [ 169810 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00027B3D0D", "query_cov": 0.8108108108108109, "subject_cov": 0.6060606060606061, "identity": 0.983, "score": 118.0, "evalue": 3.96e-33, "gene_start": 13, "gene_end": 72, "reference_start": 26, "reference_end": 85, "reference_sequence": "MTSLLKLLSSKNIKQKLGKGGTAMQGSFSVQDTFSFVVPYSGNHSNQISSGTIKTAYPVKKDQKSTVKINSLINATPLNSYGDLNNHTKLIYPNYLLIY" }, "paralog": false, "identity": 0.8947368421052632, "score": 134.0, "evalue": 4.09e-42, "description": "Internal stop codon at 169810. Frameshift due to deletion around 169807.", "psc": { "uniref90_id": "UniRef90_UPI00027B3D0D", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0030D266AC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00027B3D0D", "UniRef:UniRef50_UPI0030D266AC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0030D266AC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D266AC" ], "product": "MgpC family cytadherence protein" } }, "id": "KKNAAMEMLJ_337", "locus": "GCA000027325_00298" }, { "type": "rRNA", "sequence": "contig_1", "start": 170012, "stop": 171529, "strand": "+", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1443.6, "evalue": 1.8e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGAAGTAGCAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAAACTAGCTAATACCGCATAAGAACTTTAGTTCGCATGAATTAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTAGGGTAATGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTTTGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTCTAGCAGGCAATGGCTGGAGTTTGACTGTACCACTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATCAGTCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGAGCGATCCCTTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTTTAACGAGACTGCTAATGTAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTAGCCAACTTGTAAAAGTGAGCAAATCTGAAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGCTGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCTTTATTGGAAGCGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTT", "id": "KKNAAMEMLJ_38", "locus": "GCA000027325_00299" }, { "type": "rRNA", "sequence": "contig_1", "start": 171729, "stop": 174644, "strand": "+", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9969230769230769, "score": 2681.4, "evalue": 6.1e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "CAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAAAAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGGAGGTGGTAAAAACCTGAGATCCGGAGGTGTCCGAATGGAGCAATCTGGTAGCTTGCAAAAGTTACCATTAATTAATGAATTCATAGTTAATTAAAGCGATACGTGGTGAAGTGAAACATCTCAGTAACCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACCTATCTGAGGATAGGGGTTGTAGGGCTTGCATTATGGAAGTTAAAAGGATAGAAGAAGCTGTTGGAAAGCAGCGCCAAAGAGGGTGATAGCCCCGTATTTGAAATCTTTTTAATACCTAGCAAGAAACCTGAGTAGCTCGAAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAGAGAGCGTTTGTTTGTTATCATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATTAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTAAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTTGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATATTTTTATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAGAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTATCCACCCCCATTATTGGATTTGGGGATAAACCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACACTGAGTGGTGATGTCGAGTGAACGAGTGATCAAGTAGCGAAGGTGGCAATTAATCATGCTTTCAAGAAAAGCTGCTAGGGCTAATTTAACTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATCTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCAATTTATTGCAAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAAAACACCACCATGTAGAGAATAGGTAGGAGCAATTGATGCAAGTTCGCAAGGATTTGTTGATGTGAAATGTGGAATACTACCCTTGGTTATGTTTTGTTCTAATTGGTAACTGTGATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCACAAAGGTACCTTCAGTACGGTTGGAAATCGTATTTAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTTCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCACAGGAAGATTGAAGAGCTTTGCTTCTAGTACGAGAGGACCGGAGCGAGGACACCGCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCTGTTAAAGGAAAGTAAGAGCCGTTATAGACCATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGT", "id": "KKNAAMEMLJ_40", "locus": "GCA000027325_00300" }, { "type": "rRNA", "sequence": "contig_1", "start": 174685, "stop": 174793, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.9159663865546218, "score": 72.8, "evalue": 5.1e-12, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TTTGGTGCTAATATCGCTGTGGAAACACCTGGAACCATCCCGAACCCAGCAGTTAAGCACAGTGGAGCTAAATGTAGGTAGTAATACTGAGAATAGGTAAGCACCAAGC", "id": "KKNAAMEMLJ_39", "locus": "GCA000027325_00301" }, { "type": "cds", "sequence": "contig_1", "start": 175419, "stop": 175667, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557D08", "UniRef:UniRef90_UPI001ED8F9BD" ], "nt": "ATGACCAACTTGTTAAAACTCCTAACAACTAAAAACATCAAAGCGAAATTGGGGAAGGACACCCAATCAATGGGAAATAATAATGGAGGGGGTGTTAGTCAAACCTTTTCCTTTGTTGTTCCTTATTCAATGAATCATACAAATACGGGAACTAGTGGAACCATTAAAACTGCTTATCCAGTGAAAAAAGATGAAGCTTCCCAAGTAGCGATCAATTCCTTGATCAACGCTACGCCATTGAATAGTTAA", "aa": "MTNLLKLLTTKNIKAKLGKDTQSMGNNNGGGVSQTFSFVVPYSMNHTNTGTSGTIKTAYPVKKDEASQVAINSLINATPLNS", "aa_hexdigest": "2b09e1ba64b302c5d55e583a82cc099b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED8F9BD", "query_cov": 0.8170731707317073, "subject_cov": 1.0, "identity": 0.91, "score": 115.0, "evalue": 3.67e-32, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0000557D08" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557D08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557D08" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "KKNAAMEMLJ_338", "locus": "GCA000027325_00302" }, { "type": "cds", "sequence": "contig_1", "start": 175967, "stop": 176257, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACCAACAGGATAAACTTGAATTTAACTTAACAAGATTAAAGGAGATAGATGTTGAAGATGGTAAGCAACTATTTGGGATAGAAACTAGTGTTTATAAACACTTTCAAAATGAAATTGCTCGTTTTTATAAACAAGTAAACAAACACTTTCGTGAAACAGGTAGTGAAAGTTTGTTTTTAGCTTTACCAGTTATTGAAGGGATTAATGAGTTTAACGATATCTTTCGAGCTCCATTACTTTATGTTGGAGTTAAACTCAAAGTTTCCCCACGCTTTGAACGTTTCTGA", "aa": "MNQQDKLEFNLTRLKEIDVEDGKQLFGIETSVYKHFQNEIARFYKQVNKHFRETGSESLFLALPVIEGINEFNDIFRAPLLYVGVKLKVSPRFERF", "aa_hexdigest": "f2df0dde154fc7de8d26cbbee3a24d31", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11369.886599999989, "isoelectric_point": 6.736182212829591 }, "id": "KKNAAMEMLJ_339", "locus": "GCA000027325_00303" }, { "type": "cds", "sequence": "contig_1", "start": 176330, "stop": 177181, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGTTTAAAAATAACTATGATACTACTAAGATAGATGTTAATGATGCTTTAAAGGTATTTAGTGAACTTGAATATGAGTTTAGAATGCCTTTAACTTCTGAATTGAAGAGTTTTAGTAAAAAAGCAAAGAGTGATTTTAATACTGAAAAACGAACTAACTATCTTATTAACAACGTTCTTTTGGGGATCTTTGATGTTAAGGGTGATCAGCTGTTCCAAAACTTTAATGAGATTCTAAACACTGATCCTGATGTATTAGATGAACTTCTAAAAGATAGAAGAGATCTGTTGTTAGAAAACCGGGAATTCCGTGAACAATTTGATTTAAAAGATACCTATCTCTTCAGTCACCTTGATATCTACCAACAGTATGCAGTTAAGCAAGCTTTACTTGGTGATTTAATTATTGAAGGCCCACCTGGCACAGGGAAATCTGAAACAATTGTTAATATCTTAGTTAACCTTGTTTTAAACAACAAAAAAGTATTGTTTGTTTCTGAAAAAGTAACTGCACTTGATGTTGTTTACAACCGTCTTGGTAGTTTTAAACACATCGCACTTTTCAACGCTAGTGTTGCTGCTGAAAAGAAACGCTTTTATAACCAGTTTGCTGAGTTTGAAACTTATTTCACTACTTACTTTTCCAAGAAAGATTTGGATGCTACTTTACCAACATTTGAAGGTAAATGGGTGGATGATATTTTAGGGGCATTTCAAGCATTACAAGCTCTTTATGACACCAAGATAAATTCTGGTGAAAATCTGTTTAGTTTCAAAGAGATTGTCAGTAGCTTTCAGATGTTGGATGCTAGTTACATCAAGATTAAAGAATATGAACGTTTTGATGAGTGA", "aa": "MFKNNYDTTKIDVNDALKVFSELEYEFRMPLTSELKSFSKKAKSDFNTEKRTNYLINNVLLGIFDVKGDQLFQNFNEILNTDPDVLDELLKDRRDLLLENREFREQFDLKDTYLFSHLDIYQQYAVKQALLGDLIIEGPPGTGKSETIVNILVNLVLNNKKVLFVSEKVTALDVVYNRLGSFKHIALFNASVAAEKKRFYNQFAEFETYFTTYFSKKDLDATLPTFEGKWVDDILGAFQALQALYDTKINSGENLFSFKEIVSSFQMLDASYIKIKEYERFDE", "aa_hexdigest": "584d57a02704f1bccee052c8cfe19e65", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 73, "aa_cov": 0.22614840989399293, "hmm_cov": 1.0, "evalue": 1.2566627017545608e-09, "score": 33.314697265625, "start": 118, "stop": 181 } ], "seq_stats": { "molecular_weight": 32937.96540000003, "isoelectric_point": 4.954166221618652 }, "id": "KKNAAMEMLJ_340", "locus": "GCA000027325_00304" }, { "type": "cds", "sequence": "contig_1", "start": 177236, "stop": 177556, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAAGAACTTAGTCAGCGCTGGAATGGTATTGATAATTTCTATCAGTTGCAATCGCTTCTAAACCAAACCAAAAAACGAAAGGTCTTAAACTATGTGTTGGAACACTTTGAACAGTTTAATACAGTTATCAGTCCTAAGCATGTTTTGTTCTACAAGCCTAGCAATAAATCACAATTGCTCTTAAAACAACTGAAACAGGATGTTGAACAATACACTAGTTTACAACGTTTCCAATCTCCTACTAAGTTTGAAACAATCAAGTTGAATTTCATCAACCAAGTTAATGAAAACCCAACCCCATGGTTCTTTTCTTGA", "aa": "MKKELSQRWNGIDNFYQLQSLLNQTKKRKVLNYVLEHFEQFNTVISPKHVLFYKPSNKSQLLLKQLKQDVEQYTSLQRFQSPTKFETIKLNFINQVNENPTPWFFS", "aa_hexdigest": "60f7feb5b772c56f979b4cfafbb6c1d6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12827.560899999988, "isoelectric_point": 9.776324272155762 }, "id": "KKNAAMEMLJ_341", "locus": "GCA000027325_00305" }, { "type": "cds", "sequence": "contig_1", "start": 177584, "stop": 178276, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGGAAAAACTCGTTAGTTTTGAGTCAAACATTATTAAAACAAAACAAGCTTATCTTAATGGGATTGAAAGCTATGTAGCAAGTTGTAAGAAACTGCTTAAAACAACTATTTTAAACAACTTTTTTCAGCTTTATCAAACTAATAAAGATGAACTATTGGAGATCTGCAGACAAGCAAAAAACCCAGTTTTAAAAGAGATTACTTGGTGGTTTAAAAAACATTTTGAACTCTTAAAAAAACTCTTTCCAGTGCACATTATGACCCTTGAGTCTGCAGCAACTCTAACCCCTAACCAACGTGGTTTGTATGACTATGTGGTTATTGATGAAGCTAGTCAAGTATTTCTAGAAAGAGCAATTCCTATCTTATTTAGAGCTGATAAGTACATTATTGCAGGGGACACTAAACAGTTAAAACCAGCTAACTTCTTCCAATCACGTGCTGAGTATGATGTTGATGAGGAGTTTGAAGATGGCAATATAGAAGCTGCTGTTCACTCCAGTTCTCTACTTCATTTTTTAAAGAACCGTTCAAGAATCTTAACTTTACTTAAGTTCCACTACCGCAGTGATTCAGCTGATCTGATTGCTTTTACTAATAACAGGATCTATGACAATGAATTAATCTTTATGAATAAAGCTAATGCTGATCAAAGGGTTTTTATTGTCCATGATGTAATAGATGGTATCTGA", "aa": "MEKLVSFESNIIKTKQAYLNGIESYVASCKKLLKTTILNNFFQLYQTNKDELLEICRQAKNPVLKEITWWFKKHFELLKKLFPVHIMTLESAATLTPNQRGLYDYVVIDEASQVFLERAIPILFRADKYIIAGDTKQLKPANFFQSRAEYDVDEEFEDGNIEAAVHSSSLLHFLKNRSRILTLLKFHYRSDSADLIAFTNNRIYDNELIFMNKANADQRVFIVHDVIDGI", "aa_hexdigest": "e6aee446c4281807e0d2828a137a8f41", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 54, "aa_cov": 0.23043478260869565, "hmm_cov": 1.0, "evalue": 3.027233810938235e-10, "score": 35.340450286865234, "start": 88, "stop": 140 } ], "seq_stats": { "molecular_weight": 26793.49060000002, "isoelectric_point": 6.603235816955567 }, "id": "KKNAAMEMLJ_342", "locus": "GCA000027325_00306" }, { "type": "cds", "sequence": "contig_1", "start": 178313, "stop": 178447, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAACGCTTAGAACAACTAACAACAACCAATGATTACAAAAAGTCACTTGGTGTAATTTGTTTCAATAAAAACCAAGCAGATCTAATTGAATATCTAATTGATAAACAAAACAATCCATTACTTAACGAGTGA", "aa": "MQRLEQLTTTNDYKKSLGVICFNKNQADLIEYLIDKQNNPLLNE", "aa_hexdigest": "3284f53694d67ee22a658d358df2cd28", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5170.8263, "isoelectric_point": 5.145031547546386 }, "id": "KKNAAMEMLJ_343", "locus": "GCA000027325_00307" }, { "type": "cds", "sequence": "contig_1", "start": 178490, "stop": 179068, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "GTGAAAAACATTGAAAATGTGCAGGGTGATGAAAGGGATATCATCATCTTTTCTTTAGGTTATGATCGTTCAGTAAATAGTTATGGTCCTATTAGTAAGCAAGGTGGAGAAAACAGACTAAATGTAGCGATAACTAGAGCTAAACAAAGAATAGAACTGTTTAAGACAAATAGAGGAGAAGACTACAATGGTTTAAGCTCCAGTTCCTTGGGTAGTAAACTGTTAGTTGAATATCTGCTTTATTGTGAAGCGATGGCTAAAAACCAGGGTGAGAAAATCACTTTTCAAGCGGTAAAAAGAAAAGAAACAAAAGCAAAGTATGAACTGGCAGTTGAAAATGATTTCTTCAACCAACTGCAGGCAATTTTTGGTGGAGAGTTTGAGATTAAACGTAACGTTAATGAAGGGGCTTACTTTTTCTCATTTGTCTTTTACTTTAATAATATCCCTTATCTTGCCATTGACTTTAACATCCCCATTCCCACTTCAAGAAAACAAGTTATGGAAGGGATTTTATACCGTGAACAGTTTCTCAAAAAACGTCAATGGAACCTAATTAACATCTGGATTGATGAGTGA", "aa": "MKNIENVQGDERDIIIFSLGYDRSVNSYGPISKQGGENRLNVAITRAKQRIELFKTNRGEDYNGLSSSSLGSKLLVEYLLYCEAMAKNQGEKITFQAVKRKETKAKYELAVENDFFNQLQAIFGGEFEIKRNVNEGAYFFSFVFYFNNIPYLAIDFNIPIPTSRKQVMEGILYREQFLKKRQWNLINIWIDE", "aa_hexdigest": "1457f84b849b54c1a02dc0eef28a2bde", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 50, "aa_cov": 0.2604166666666667, "hmm_cov": 0.94, "evalue": 4.848291045165928e-09, "score": 31.11225128173828, "start": 2, "stop": 51 } ], "seq_stats": { "molecular_weight": 22372.20070000001, "isoelectric_point": 8.510100746154787 }, "id": "KKNAAMEMLJ_344", "locus": "GCA000027325_00308" }, { "type": "cds", "sequence": "contig_1", "start": 179151, "stop": 179834, "strand": "+", "frame": 3, "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "db_xrefs": [ "COG:COG0195", "COG:K", "GO:0003700", "GO:0003723", "GO:0005829", "GO:0006353", "GO:0031564", "SO:0001217", "UniRef:UniRef50_P47387", "UniRef:UniRef90_P47387" ], "nt": "ATGAAGATAACTTTCATTTCTGGACAAGAAGTGTCGTTAGGCACTTCTTTTTTATTGTTTTCAAAAAAAATAGTTATGAATGAATTAAACCAACCCTTACTTGCTATTATTAAAAATGTTGCTAAAACCAAAAACCTTTCTATAGAAGAGGTGGTTTTTTGTTTGAAAACAGCTTTAGAACAAGCCTATAAAAAACACCTTAACTTTGTTAATGTTGAAGTTAACATTAACTTTGATAAGGGGATTATTAATGTTGAACAACTCTTTAATGTTGTTAGTGATGAAAATGAAGATTATGATGACTTTCTTGAAATCCCTTTACAAGCAGCTAACAAAATAAACAGTTCATTGCAATTAGGTGATGTGTTGCGAAAACCAATCCCCTTAAAAAACATTAGTAGTGATCTTATCAATAAGATGATTGCTATCTTTAACCAAAAGATTAGTGAAACAAACTTTAAAGCAGTAATGAGTGAGTTTAGTAGTGAGGTTGGGGAAGTGATTGAAGCGAAAGTTGAAGATATTGATACTAACAAAGAAGGTGGTTTAAAGGGTTATATTATTAACCTTGAAACTACAAAGGGTTATATCTCCAAGCGGGAATTGTCAAAAGGGGAGCGCTTAGAGATAGGTAAAAAATACCTCTTTGTTATCAAAGAAATCCAACGGCAAGCATCGTTATGA", "aa": "MKITFISGQEVSLGTSFLLFSKKIVMNELNQPLLAIIKNVAKTKNLSIEEVVFCLKTALEQAYKKHLNFVNVEVNINFDKGIINVEQLFNVVSDENEDYDDFLEIPLQAANKINSSLQLGDVLRKPIPLKNISSDLINKMIAIFNQKISETNFKAVMSEFSSEVGEVIEAKVEDIDTNKEGGLKGYIINLETTKGYISKRELSKGERLEIGKKYLFVIKEIQRQASL", "aa_hexdigest": "fc7b80363a74d6a3f48bff6e283178bd", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusA" ], "truncated": "3-prime", "pseudogene": { "start": 179151, "stop": 180137, "observations": { "mutation": [ 179832 ], "stop": [ 179832 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47387", "query_cov": 1.0, "subject_cov": 0.4274952919020716, "identity": 1.0, "score": 426.0, "evalue": 8.37e-146, "gene_start": 1, "gene_end": 227, "reference_start": 1, "reference_end": 227, "reference_sequence": "MKITFISGQEVSLGTSFLLFSKKIVMNELNQPLLAIIKNVAKTKNLSIEEVVFCLKTALEQAYKKHLNFVNVEVNINFDKGIINVEQLFNVVSDENEDYDDFLEIPLQAANKINSSLQLGDVLRKPIPLKNISSDLINKMIAIFNQKISETNFKAVMSEFSSEVGEVIEAKVEDIDTNKEGGLKGYIINLETTKGYISKRELSKGERLEIGKKYLFVIKEIQRQASLWPITLSRSDTRLLQFLLTSNTPEIENGTIVIKKIERSPGVKSKIAVISNDPAVDPVAAILGPKGEKIRGISEEFNGEIIDIVFWNEDKLKFLINAILPAEVIGYNILQDDERDTSIEVVVPANQIANVFGFKGVNIRLISNLTGWNSVDVYSEKDASEANIKFTRLSFEPEGLFGIKKRREKIISNDATDKVFYTSKDNVIDDEIIVDLAKDLMVDNKQKQPEQVAKQVVEKSQLEKQVTPKEKEKVQPKAKVHSNSHSKKPAKPNQIFSITVDASDKNLKKDQVDNNQTNPQTKQTFDSFDDL" }, "paralog": false, "identity": 0.9969512195121951, "score": 625.0, "evalue": 1.33e-226, "description": "Internal stop codon at 179832. Nonsense mutation around 179832.", "psc": { "uniref90_id": "UniRef90_P47387", "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "uniref50_id": "UniRef50_P47387", "cog_id": "COG0195", "cog_category": "K", "go_ids": [ "GO:0005829", "GO:0003700", "GO:0003723", "GO:0006353", "GO:0031564" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47387", "UniRef:UniRef50_P47387", "COG:COG0195", "COG:K", "GO:0005829", "GO:0003700", "GO:0003723", "GO:0006353", "GO:0031564" ] }, "pscc": { "uniref50_id": "UniRef50_P47387", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47387" ], "product": "Transcription termination/antitermination protein NusA" } }, "id": "KKNAAMEMLJ_345", "locus": "GCA000027325_00309" }, { "type": "cds", "sequence": "contig_1", "start": 179877, "stop": 180266, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00013.35", "PFAM:PF13184.12" ], "nt": "TTGTTAACTTCAAATACTCCAGAAATTGAAAATGGTACGATTGTAATCAAAAAGATTGAACGTTCCCCAGGAGTGAAATCAAAGATAGCAGTTATCTCCAATGATCCTGCAGTTGACCCAGTTGCTGCTATCTTAGGACCTAAGGGTGAGAAGATTAGGGGGATTAGTGAGGAATTTAATGGTGAGATTATTGACATTGTCTTTTGGAATGAAGACAAGTTAAAGTTCTTAATTAATGCCATTTTACCTGCAGAAGTCATTGGTTATAACATCTTGCAGGATGATGAGCGTGATACTAGTATTGAAGTTGTTGTACCTGCAAACCAAATTGCTAATGTTTTTGGTTTTAAAGGTGTAAACATTAGGTTAATTAGTAATTTAACAGGTTGA", "aa": "MLTSNTPEIENGTIVIKKIERSPGVKSKIAVISNDPAVDPVAAILGPKGEKIRGISEEFNGEIIDIVFWNEDKLKFLINAILPAEVIGYNILQDDERDTSIEVVVPANQIANVFGFKGVNIRLISNLTG", "aa_hexdigest": "d48c40d913118e7b7dc7df232dcadd95", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00013.35", "name": "KH_1", "length": 27, "aa_cov": 0.20930232558139536, "hmm_cov": 0.9629629629629629, "evalue": 5.318202064399279e-09, "score": 30.86907196044922, "start": 41, "stop": 67 }, { "id": "PF13184.12", "name": "KH_5", "length": 66, "aa_cov": 0.5116279069767442, "hmm_cov": 1.0, "evalue": 9.571078223120872e-23, "score": 75.27362823486328, "start": 23, "stop": 88 } ], "seq_stats": { "molecular_weight": 14041.997699999982, "isoelectric_point": 4.736416053771973 }, "id": "KKNAAMEMLJ_346", "locus": "GCA000027325_00310" }, { "type": "cds", "sequence": "contig_1", "start": 180348, "stop": 180746, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGCATCAAAAAAAGAAGGGAAAAGATCATTAGTAATGATGCTACTGATAAAGTCTTTTACACCTCTAAAGACAATGTGATAGATGATGAGATTATTGTTGATTTAGCTAAAGATCTAATGGTTGATAATAAACAAAAACAACCTGAGCAAGTTGCAAAGCAAGTTGTTGAAAAATCACAATTAGAAAAACAAGTTACTCCAAAAGAAAAAGAGAAAGTTCAACCAAAAGCTAAGGTTCATTCTAATAGCCATTCCAAAAAACCAGCTAAACCTAATCAGATTTTTTCTATCACTGTTGATGCTAGTGATAAGAATCTTAAAAAAGATCAAGTTGATAATAACCAAACAAACCCCCAAACAAAACAAACATTTGATAGCTTTGATGATCTTTAA", "aa": "MFGIKKRREKIISNDATDKVFYTSKDNVIDDEIIVDLAKDLMVDNKQKQPEQVAKQVVEKSQLEKQVTPKEKEKVQPKAKVHSNSHSKKPAKPNQIFSITVDASDKNLKKDQVDNNQTNPQTKQTFDSFDDL", "aa_hexdigest": "0733e5c815ecc8572b3ab59a4550d869", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15126.898899999982, "isoelectric_point": 8.961314964294434 }, "id": "KKNAAMEMLJ_347", "locus": "GCA000027325_00311" }, { "type": "cds", "sequence": "contig_1", "start": 180746, "stop": 181018, "strand": "+", "frame": 2, "gene": "ylxR", "product": "putative protein MG141-1", "db_xrefs": [ "COG:AJ", "COG:COG2740", "RefSeq:WP_009885828.1", "SO:0001217", "UniParc:UPI0000557DAE", "UniRef:UniRef100_Q2MHT0", "UniRef:UniRef50_Q2MHT0", "UniRef:UniRef90_Q2MHT0" ], "nt": "ATGCAACTAATAACAAGACTTTGTTTATTAACAAGAAAACATTTTGTTAAAAGAGAACTTTTACGTCTTGTAAAATTAGACAACCAACTTGAAATTGATCTTAATCAAAATCTCAAGGGCAGGGGTTATTATTTGAGTGTTTTTGGTTTAAAGCTAGATAAAAAACACCTCAAAGCTGTAGTTGAAAAACACCTTAAGGTTAGTTGTAATGATGCAAAGCTTACTGCAATGATTACCGCCTTACAACAATTAGCACAAGATGAAAAAAAATAG", "aa": "MQLITRLCLLTRKHFVKRELLRLVKLDNQLEIDLNQNLKGRGYYLSVFGLKLDKKHLKAVVEKHLKVSCNDAKLTAMITALQQLAQDEKK", "aa_hexdigest": "99d2c3eec803f3ec69b4cb1a48b15476", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557DAE", "ncbi_nrp_id": "WP_009885828.1", "uniref100_id": "UniRef100_Q2MHT0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557DAE", "RefSeq:WP_009885828.1", "UniRef:UniRef100_Q2MHT0" ] }, "ips": { "uniref100_id": "UniRef100_Q2MHT0", "uniref90_id": "UniRef90_Q2MHT0" }, "psc": { "uniref90_id": "UniRef90_Q2MHT0", "gene": "YlxR", "product": "Uncharacterized protein MG141.1", "uniref50_id": "UniRef50_Q2MHT0", "cog_id": "COG2740", "cog_category": "AJ" }, "pscc": { "uniref50_id": "UniRef50_Q2MHT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2MHT0" ], "product": "Uncharacterized protein MG141.1" }, "genes": [ "ylxR" ], "id": "KKNAAMEMLJ_348", "locus": "GCA000027325_00312" }, { "type": "cds", "sequence": "contig_1", "start": 181005, "stop": 182864, "strand": "+", "frame": 3, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413", "KEGG:K02519", "RefSeq:WP_010869352.1", "SO:0001217", "UniParc:UPI000012D2DF", "UniRef:UniRef100_P47388", "UniRef:UniRef50_P47388", "UniRef:UniRef90_P47388" ], "nt": "ATGAAAAAAAATAGAGCTTTCAATCAGGTTAAAAAAACAAAGTTTGACGGTAGGATTAAAACCAGTGCCAAACACCAGTTACGTAATGTTAAAACCGGGGTTAAAGATGGTGTTTTTATCTATAAAGGTCCTTTAACTGTTAGTGAGTTTGCAAGTAAAACTAATATCGCTGTTGCTAACATTATCAAACACTTTTTTTTAAATGGTTTGGCACTAACAGTTAATTCAGTTTTAACAAATGAACAGTTAGCAGATGCATGTGTTAACTTTGGGTTTGACTTTAAGATGGAAACTGAAGTTACCCATGAAAATATTGTAGCTAACATCCAGTTTGAAGATAGTGATGATTTATTGCAACCAAGACCACCTATTGTTACTATCATGGGTCATGTTGACCATGGTAAAACTTCGCTTTTAGACACAATTAGAAAAACTAATGTAACTGCTAAGGAGTTTGGCGGAATTACCCAAAAAATTGGTGCTTATCAGGTGAAAAATCACCAAAATAAAACGATTACTTTTATTGATACTCCTGGGCATGAAGCATTTACTTTAATGCGTGCAAGGGGTGCAAAAGTAACTGATATTGTGGTGTTGGTTGTGGCAGCGGATGATGGGATTAAAAAGCAAACAGAGGAAGCAATTAGCCATGCTAAGAGTGCTAACACTCCTATCATTGTTTTTATTAACAAGATGGATAAACCAACTGCTAACCCTGATCTGGTGATCCAACAACTCAATAAGTTTGATTTAGTTCCTGAGGCTTGGGGTGGGAAAACTATCTTTGTAATGGGTAGTGCTTTAACTGGTCAAGGGATTAATGAGTTGCTTGATAATATCTTGTTGCTAGGGGAAGTGGAGGGTTATCAAGCTAACTATAATGCCCATTCATCTGGTTATGCAATTGAAGTACAAACTTCAAAGGGACTTGGCCCTATTGCCAATGTCATTGTAAAAAGGGGTACTTTAAAGTTAGGTGACATTGTGGTGTTAGGGCCTGCATATGGAAGAGTTAGAACGATGCATGATGAAAATGGTAATAGCTTAAAACAAGCAACCCCTTCAAAACCTGTGCAGATCTCAGGGTTTGACATTATGCCTGTTGCTGGGGAAAAGTTCATTGTTTTTGATGATGAGAAGGATGCAAAGTTAATTGCTAACAAGTTTAAAGAACAACAAAAACAAAAAGCTAACAACTTAACAGTTAATCAAACCTTAAAAGAACAGATTAAAAACAAGGAAATTAAGATATTAAATTTGATCTTTAAAGCAGATAGTGATGGTTCATTGCAAGCTATTAAACAAGCAGTTGAAAACATTAATGTTGCTAAGATCTCACTTAGTATCATCCATGCTGCAGTGGGGCAGATATCAGAGAGTGATATTATGCTAGCAAAAGCATCAGGGGCTTTATTGTTTAGTTTAAACTTAGGTTTGAGTCAAACTGTAAAAAACATTGCTAGTTTACAAGGGGTAAAATTAGAAGTTCACTACCATATCCCTAAACTAGCAGAGGAGATTGAAAACATCTTAAAAGGTCAATTAGATCCTGTTTATGAAGAGATTGAAATAGGTAAAGCGGAAGTTTTACAACTCTGGTTCCACTCTAAAATCGGTAATATTGCAGGAACCATTGTTAAATCAGGAAAGATAAAAAGAGGGAATTTATGTAAGTTATTCAGAGATAAAGAGATTATCTTTGAAGGCAGAATTGACTCTTTAAAAAATGAAAAAACGCCTGTTAATTTAATAGAAACAGGGAAGAATTGTGGGATAGTTATTAATGGTTGCAATGATATTAAGATTGGTGATATCATTGTTGCTTATGAAAAGCAGATAGTTAAAGATGGCAAGCTATAG", "aa": "MKKNRAFNQVKKTKFDGRIKTSAKHQLRNVKTGVKDGVFIYKGPLTVSEFASKTNIAVANIIKHFFLNGLALTVNSVLTNEQLADACVNFGFDFKMETEVTHENIVANIQFEDSDDLLQPRPPIVTIMGHVDHGKTSLLDTIRKTNVTAKEFGGITQKIGAYQVKNHQNKTITFIDTPGHEAFTLMRARGAKVTDIVVLVVAADDGIKKQTEEAISHAKSANTPIIVFINKMDKPTANPDLVIQQLNKFDLVPEAWGGKTIFVMGSALTGQGINELLDNILLLGEVEGYQANYNAHSSGYAIEVQTSKGLGPIANVIVKRGTLKLGDIVVLGPAYGRVRTMHDENGNSLKQATPSKPVQISGFDIMPVAGEKFIVFDDEKDAKLIANKFKEQQKQKANNLTVNQTLKEQIKNKEIKILNLIFKADSDGSLQAIKQAVENINVAKISLSIIHAAVGQISESDIMLAKASGALLFSLNLGLSQTVKNIASLQGVKLEVHYHIPKLAEEIENILKGQLDPVYEEIEIGKAEVLQLWFHSKIGNIAGTIVKSGKIKRGNLCKLFRDKEIIFEGRIDSLKNEKTPVNLIETGKNCGIVINGCNDIKIGDIIVAYEKQIVKDGKL", "aa_hexdigest": "b62d9fedc9d3334548359bb3280ff177", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012D2DF", "ncbi_nrp_id": "WP_010869352.1", "uniref100_id": "UniRef100_P47388", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D2DF", "RefSeq:WP_010869352.1", "UniRef:UniRef100_P47388" ] }, "ips": { "uniref100_id": "UniRef100_P47388", "uniref90_id": "UniRef90_P47388" }, "psc": { "uniref90_id": "UniRef90_P47388", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_P47388", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_P47388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47388" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "KKNAAMEMLJ_349", "locus": "GCA000027325_00313" }, { "type": "cds", "sequence": "contig_1", "start": 182851, "stop": 183189, "strand": "+", "frame": 1, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0042254", "GO:0043024", "KEGG:K02834", "RefSeq:WP_009885829.1", "SO:0001217", "UniParc:UPI00001331AF", "UniRef:UniRef100_P47389", "UniRef:UniRef50_P47389", "UniRef:UniRef90_P47389" ], "nt": "ATGGCAAGCTATAGAAAACAACGGATTGAAAATGATATCATCCGCTTAATTAATCGCACGATTATTAATGAGATCTATGATCCTGTTGTTAAGTTAGGTCATGTTAGCCATGTGAAGTTATCAGCTGATTTTTTTCATGCAGTAGTTTATCTTGATTGTTATGATCGTAGTCAGATTCAAACTGTAGTTAATGCTTTTAAAAAGGCTCAGGGCGTTTTTAGTCAAATGTTAGCACAAAATTTGTACCTAGCTAAAAGTGTAAAACTCCACTTTGTGAAGGATGATGCAATTGACAATGCTTTGAAAATAGAACAGATAATTAACTCTTTGAAAAACTAA", "aa": "MASYRKQRIENDIIRLINRTIINEIYDPVVKLGHVSHVKLSADFFHAVVYLDCYDRSQIQTVVNAFKKAQGVFSQMLAQNLYLAKSVKLHFVKDDAIDNALKIEQIINSLKN", "aa_hexdigest": "4500bbaa521ddaacfdfd8700f355eab2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001331AF", "ncbi_nrp_id": "WP_009885829.1", "uniref100_id": "UniRef100_P47389", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001331AF", "RefSeq:WP_009885829.1", "UniRef:UniRef100_P47389" ] }, "ips": { "uniref100_id": "UniRef100_P47389", "uniref90_id": "UniRef90_P47389" }, "psc": { "uniref90_id": "UniRef90_P47389", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_P47389", "cog_id": "COG0858", "cog_category": "J", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0005829", "GO:0006364", "GO:0030490", "GO:0042254", "GO:0043024" ] }, "pscc": { "uniref50_id": "UniRef50_P47389", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47389" ], "product": "Ribosome-binding factor A" }, "genes": [ "rbfA" ], "id": "KKNAAMEMLJ_350", "locus": "GCA000027325_00314" }, { "type": "cds", "sequence": "contig_1", "start": 183214, "stop": 183639, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG144", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47390", "UniRef:UniRef90_P47390" ], "nt": "ATGGACCCACAAAACAAGTCCCCAAAACCACAAGTTAAATCAACTAGATTGGTTGTCAAAAAACAACCAGCAGGTGTTGTTTTTCCCAAATTGAGTATTCCTGTTAATGATTTTGAAAAAACAGTTACTTTAACAAGAGCACAAAAAAAGGAAGCTAAACTTCTCAAAAAAGCCCAAAGGAAAGCTAATAAGCTTAATAATAAACAAGATAGTACCTTTTTCAATTCTGCTTCAGGTGAGACTAATAACACCATTCTTCCTCCTGGTGTTAAAAATCAAGCTGATAATAAAACTAACCGTTTTAGTAAATTTATTAGTTTTTTCACTTCTTCAAAAAACAAACAACCAGATGAAATAACAGAAAGGTTAGTTGATGATCCTACTGTTAAAAACCGTTTTAGTGCTTTTAATAAGAAGCTTATTTGA", "aa": "MDPQNKSPKPQVKSTRLVVKKQPAGVVFPKLSIPVNDFEKTVTLTRAQKKEAKLLKKAQRKANKLNNKQDSTFFNSASGETNNTILPPGVKNQADNKTNRFSKFISFFTSSKNKQPDEITERLVDDPTVKNRFSAFNKKLI", "aa_hexdigest": "235ac0632cab6430a297a537f3864b80", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 183214, "stop": 183942, "observations": { "mutation": [ 183637, 183811, 183856 ], "stop": [ 183637, 183811, 183856 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47390", "query_cov": 1.0070921985815602, "subject_cov": 0.5071428571428571, "identity": 0.972, "score": 259.0, "evalue": 3.86e-85, "gene_start": 1, "gene_end": 141, "reference_start": 1, "reference_end": 142, "reference_sequence": "MDPQNKSPKPPVKSTRLVVKKQPAGVVFPKLSIPVNDFEKTVTLTRAQKKEAKLLKKAQRKANKLNNKQDSIFFNSASVGDTNNTILPPGVKNQADNKTNRFSKFISFFTSSKNKQPDEITERLVDDPTVKNRFSAFNKKLIWVLKDKKLRARAWKIVGYTNLVIVAFFAGLLAVMNKFITLPSVEYPAIALQLPINNALWGISIFVISIVTLPFWTMFILFLMGVKDVRTSRSIHYFIWIVLIINVVLLLVSCLLMIAAYAHLDGYNIWRNLESLNPNN" }, "paralog": false, "identity": 0.9670781893004116, "score": 449.0, "evalue": 9.25e-163, "description": "Internal stop codon at 183637, 183811, 183856. Nonsense mutation around 183637, 183811, 183856.", "psc": { "uniref90_id": "UniRef90_P47390", "product": "Uncharacterized protein MG144", "uniref50_id": "UniRef50_P47390", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47390", "UniRef:UniRef50_P47390", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47390", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47390" ], "product": "Uncharacterized protein MG144" } }, "id": "KKNAAMEMLJ_351", "locus": "GCA000027325_00315" }, { "type": "cds", "sequence": "contig_1", "start": 183706, "stop": 183813, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCATTTTTTGCTGGACTTTTAGCAGTGATGAATAAGTTCATCACCCTTTCCTCAGTTGAATATCCTGCTATTGCTTTACAACTCCCTATTAACAATGCATTATGA", "aa": "MAFFAGLLAVMNKFITLSSVEYPAIALQLPINNAL", "aa_hexdigest": "9d5085af04e692f4f40cff4bc8dfbbab", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3782.5132000000003, "isoelectric_point": 5.752754020690918 }, "id": "KKNAAMEMLJ_352", "locus": "GCA000027325_00316" }, { "type": "cds", "sequence": "contig_1", "start": 183862, "stop": 184020, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATCTTGTTTTTAATGGGAGTAAAGGATGTGAGAACTTCGCGTTCTATCCATTATTTTATCTGGATAGTGTTAATTATTAATGTAGTTTTATTACTAGTTAGTTGCTTGTTGATGATTGCTGCTTATGCCCATCTTGATGGTTATAACATCTGA", "aa": "MFILFLMGVKDVRTSRSIHYFIWIVLIINVVLLLVSCLLMIAAYAHLDGYNI", "aa_hexdigest": "96b80d41985d832101b1e57399461bd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6013.336699999999, "isoelectric_point": 7.959798622131348 }, "id": "KKNAAMEMLJ_353", "locus": "GCA000027325_00317" }, { "type": "cds", "sequence": "contig_1", "start": 184053, "stop": 184862, "strand": "+", "frame": 3, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0006771", "GO:0008531", "GO:0009231", "GO:0009398", "KEGG:K11753", "RefSeq:WP_009885831.1", "SO:0001217", "UniParc:UPI00001338E0", "UniRef:UniRef100_P47391", "UniRef:UniRef50_P47391", "UniRef:UniRef90_P47391" ], "nt": "ATGAAATCACTCTTTATTGGTTATTTTGATGGATTACATCAAGGTCATCTATTTTTAAAGCAGAACAGTAAGTTTGAACCAATGGTGTTATTAATTGATAACCCACCTTTAAAACAAACCAACTGGCTTTATGATTTACAACAACGGGTTGCACAAATAAAAACTTACTTGAAAGCAACTGTAGAAGTATTTGATGTTGCCAAACATAACATGAATGCACTTAGTTTTTTTGAACAACAGATTAAAAGATTGAATTGTGATGAAATTATTGTTGGTACAGATTGGCATTTTGGTAATGATCATAAGGATGGGATCTGGTTAAAGAAACTGTTTAAAAATACTGTTATTGTTAATAAAACAAACCTATCAAGTAGTGTTATCCGTAACTATCTAACTAATAATGAACTTGAAAAAGCTAACCAACTTTTAGTGGAACCTTATTATAGAGTGGGCACAGTAGTACATGGTTTAAAAAAGGCAAGGTTGCTTGGTTTTCCAACTGCTAACATTGTTATGGATAACCACTTATTGACTTTAAATAAGGGGAGTTATATAGTAAGAGTTTTATTAAATAACCAAACTTTTTATGGGATTGGTTTTATTAGCCAAAAGGATCAGGATTTGGTGTGTGAAACCCATATCTTTAACTTTAATAATGAGATTTATGGTTCACTGGTCAAATTTACACTGTTAAAGTTCATTAGAACAATTAGTAAGTTTTCCAGTCAAGCAGCTTTGCAAAAAGCAATTCAAAGTGATGCTAACTTTGCTTTAAAGTGGTTGGAAAACCAAAATTTAGATAAAATTTAA", "aa": "MKSLFIGYFDGLHQGHLFLKQNSKFEPMVLLIDNPPLKQTNWLYDLQQRVAQIKTYLKATVEVFDVAKHNMNALSFFEQQIKRLNCDEIIVGTDWHFGNDHKDGIWLKKLFKNTVIVNKTNLSSSVIRNYLTNNELEKANQLLVEPYYRVGTVVHGLKKARLLGFPTANIVMDNHLLTLNKGSYIVRVLLNNQTFYGIGFISQKDQDLVCETHIFNFNNEIYGSLVKFTLLKFIRTISKFSSQAALQKAIQSDANFALKWLENQNLDKI", "aa_hexdigest": "1b90d804a9a9d34cfb6cb35101a8d0e9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001338E0", "ncbi_nrp_id": "WP_009885831.1", "uniref100_id": "UniRef100_P47391", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001338E0", "RefSeq:WP_009885831.1", "UniRef:UniRef100_P47391" ] }, "ips": { "uniref100_id": "UniRef100_P47391", "uniref90_id": "UniRef90_P47391" }, "psc": { "uniref90_id": "UniRef90_P47391", "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_P47391", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "go_ids": [ "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0006771", "GO:0008531", "GO:0009231", "GO:0009398" ] }, "pscc": { "uniref50_id": "UniRef50_P47391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47391" ], "product": "Bifunctional riboflavin kinase/FMN adenylyltransferase" }, "genes": [ "ribF" ], "id": "KKNAAMEMLJ_354", "locus": "GCA000027325_00318" }, { "type": "cds", "sequence": "contig_1", "start": 184875, "stop": 185000, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAGTGCCCCCAGTGGTTTAACTTTAACTGTTATTATCCTTAGCATCATTCTGCTTGCTTTTATCAGCACAGTTGTATCAGCTTATGAAACAGCAATCACTTCTTTAACCCCTTACAGGTGA", "aa": "MDSAPSGLTLTVIILSIILLAFISTVVSAYETAITSLTPYR", "aa_hexdigest": "aa09da1cac1cae4958c9e0a811a6736d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4343.083299999999, "isoelectric_point": 4.369065666198731 }, "id": "KKNAAMEMLJ_355", "locus": "GCA000027325_00319" }, { "type": "cds", "sequence": "contig_1", "start": 185124, "stop": 185324, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCTAACATCCTTTTTTTAGCACTAGAACAAACAATTAAAAATGAGCTTTTATCAAGTGTTTTAAATTTGGTAGTTAGTGGGGTTTTAATCGTCTCTTTTTGTGAAATTCTACCCAAAACTTTGGGCAGAATTAATGTGATTAGAACCCTGGTTCTATTTGCTTATTTGGTTTATTTTTTTTATCTGATCTTTTGA", "aa": "MVSNILFLALEQTIKNELLSSVLNLVVSGVLIVSFCEILPKTLGRINVIRTLVLFAYLVYFFYLIF", "aa_hexdigest": "704535b5478442b3e2e21541772bb6e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7526.074599999995, "isoelectric_point": 7.899520683288574 }, "id": "KKNAAMEMLJ_356", "locus": "GCA000027325_00320" }, { "type": "cds", "sequence": "contig_1", "start": 185520, "stop": 185915, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00571.33" ], "nt": "GTGGCTTATTGTTATCTTAATGACAGTATTAACTTGATTGCCAAGCAGTTTTTACAAAGGCAGTTTTCCAGAATGCCAGTAGTAGATAAAACTACTAATAAGATAGTTGGTTTTATCCATTTAAAGGATTTTTTTACAGCTAAAGAAGCAAACCCTAAGTCACTTGATTTAAACCAGTTGCTTTATCCAGTTGTTTTAGTTCAAGATTCCACCCCCATCAAACAAGCACTAAGACAGATGCGTTTAAACAGAGCACATTTAGCAGTTGTTAATGATAAACATGAAAAAACAATAGGGATTGTTTCTATGGAGGATATTATTGAAGAGTTGGTGGGTGAAATCTATGATGAACATGATGATATCCAACCGATCCAAGTATTGGATGAAAATGTTTGA", "aa": "MAYCYLNDSINLIAKQFLQRQFSRMPVVDKTTNKIVGFIHLKDFFTAKEANPKSLDLNQLLYPVVLVQDSTPIKQALRQMRLNRAHLAVVNDKHEKTIGIVSMEDIIEELVGEIYDEHDDIQPIQVLDENV", "aa_hexdigest": "dd76cfc44f6e4081f63e19320a9ea60c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00571.33", "name": "CBS", "length": 50, "aa_cov": 0.37404580152671757, "hmm_cov": 0.98, "evalue": 2.1884953222674135e-20, "score": 67.8914566040039, "start": 63, "stop": 111 } ], "seq_stats": { "molecular_weight": 15109.248199999989, "isoelectric_point": 5.275022315979004 }, "id": "KKNAAMEMLJ_357", "locus": "GCA000027325_00321" }, { "type": "cds", "sequence": "contig_1", "start": 185973, "stop": 186149, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAATCAAAAAATATTACTATCCAGCATTATCTCGCTTCACTAGATAATGATAGTTTTGCTTGCCAAAATAAGCTTGACACTCCCTTATTTAGTGTTGAAGTGATAGCTGATAGCGAAGATAAAACCAAAATTCTTTACGAAATTAGAAAGAAGAGTGATGTTATTGCTTAG", "aa": "MVKSKNITIQHYLASLDNDSFACQNKLDTPLFSVEVIADSEDKTKILYEIRKKSDVIA", "aa_hexdigest": "623a728d4b378e88c1b6ba185ec7bab2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6588.471799999999, "isoelectric_point": 5.628106117248534 }, "id": "KKNAAMEMLJ_358", "locus": "GCA000027325_00322" }, { "type": "cds", "sequence": "contig_1", "start": 186136, "stop": 186789, "strand": "+", "frame": 1, "gene": null, "product": "MPN160 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47393", "UniRef:UniRef90_P47393" ], "nt": "ATGTTATTGCTTAGAGCATTATTTTTAGAGTTGAAAACCAACAAAAATTGTAAAGCTTTACTTTTGTTATTAATCCCCTTACTAGTTGGTTTAACCTTGATAATCTATGGGATTGTCTTATTTTCCACTGAAGGGGTAATTGACCATGGTGATCATAACCACTTAAGAGCAAGGTTTCAACTCACTTTAGAGGAGATTATTGTTTTTGTTGTTGGTAGTATTATCTTGTTTTTTACTTTAGCTAGCTTTTGTGTGAGTTGCTTTATGTTAATGAGAAGTCCTAAGCAAAAACAGCTAGAGGTTGATCATGCTAATAAAACTAATTTAAAACCAAAAGCAATAGTTAATTGTGATCTTTTTCAGTTGGGTGATTACTGTGTGTTTACATTTAAAAAACTTAGCTTTAAACAACGGTTTAAGCAAGATTTTTTTGCTAGAAGTAAGTTTTCGTTTCGCAGTGAACTGTATCGTCTTTGTTTGGTGGGAGTGTTAATTGCTCTCAATTTAGCGTTAAGTTTGATTGAAATTCCTGGGATAGTTTTACCTTGGGGTAGTTCGATCCAATTCCGTTTTTTTAATACGGCTATCTTGTTTATTGCTGTTAGGTTGGTGGGATTATTATCTACTTCTTTAGTGGCTTTAATCACCCCCTGA", "aa": "MLLLRALFLELKTNKNCKALLLLLIPLLVGLTLIIYGIVLFSTEGVIDHGDHNHLRARFQLTLEEIIVFVVGSIILFFTLASFCVSCFMLMRSPKQKQLEVDHANKTNLKPKAIVNCDLFQLGDYCVFTFKKLSFKQRFKQDFFARSKFSFRSELYRLCLVGVLIALNLALSLIEIPGIVLPWGSSIQFRFFNTAILFIAVRLVGLLSTSLVALITP", "aa_hexdigest": "c74e5bac676bd9d08eae815fbff10e3a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 186136, "stop": 187092, "observations": { "mutation": [ 186787, 186976 ], "stop": [ 186787, 186976 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47393", "query_cov": 1.0, "subject_cov": 0.5786666666666667, "identity": 1.0, "score": 401.0, "evalue": 1.17e-138, "gene_start": 1, "gene_end": 217, "reference_start": 1, "reference_end": 217, "reference_sequence": "MLLLRALFLELKTNKNCKALLLLLIPLLVGLTLIIYGIVLFSTEGVIDHGDHNHLRARFQLTLEEIIVFVVGSIILFFTLASFCVSCFMLMRSPKQKQLEVDHANKTNLKPKAIVNCDLFQLGDYCVFTFKKLSFKQRFKQDFFARSKFSFRSELYRLCLVGVLIALNLALSLIEIPGIVLPWGSSIQFRFFNTAILFIAVRLVGLLSTSLVALITPWLHLLIHPIHTPISSLFYMVNDFLVLWIFYFFFFHLFKAEVIQTTTVVDNKPFSQLVNTKKTKWTKFFSLLVISFLCGFIEGLGFYFGYFLILGNVSSLGLKIYYDGLQQRDLINSSNVLFFLMTTTAIFSIKYIFEMLFFFSVEKNVVNIANHFGLY" }, "paralog": false, "identity": 0.9937106918238994, "score": 620.0, "evalue": 2.11e-227, "description": "Internal stop codon at 186787, 186976. Nonsense mutation around 186787, 186976.", "psc": { "uniref90_id": "UniRef90_P47393", "product": "MPN160 family protein", "uniref50_id": "UniRef50_P47393", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47393", "UniRef:UniRef50_P47393", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47393" ], "product": "Uncharacterized protein MG147" } }, "id": "KKNAAMEMLJ_359", "locus": "GCA000027325_00323" }, { "type": "cds", "sequence": "contig_1", "start": 187012, "stop": 187263, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTGGCTTTATTGAGGGGTTAGGTTTTTACTTTGGTTATTTTTTAATCCTTGGTAATGTTTCTAGTTTAGGATTGAAGATCTATTATGATGGGTTACAACAACGTGATCTGATTAACAGTAGTAATGTTTTGTTCTTTTTAATGACAACAACTGCTATCTTCAGTATTAAATACATCTTTGAGATGTTATTTTTCTTTAGTGTTGAAAAGAATGTAGTTAATATTGCTAACCACTTTGGTTTGTATTAA", "aa": "MCGFIEGLGFYFGYFLILGNVSSLGLKIYYDGLQQRDLINSSNVLFFLMTTTAIFSIKYIFEMLFFFSVEKNVVNIANHFGLY", "aa_hexdigest": "6ef975fa2b9796bce3755a4651fb6480", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9566.094799999993, "isoelectric_point": 5.538129997253416 }, "id": "KKNAAMEMLJ_360", "locus": "GCA000027325_00324" }, { "type": "cds", "sequence": "contig_1", "start": 187302, "stop": 187454, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTATGTAAAAGTACAGATCATCAATAAATCAACTATTGAACTGTTAGAGGATGCTAAAAAAGGGGAAAAAATTAATTTAGATTTAATTAACCAAGTTGATCAGACCAACATCCTCAATACGATCACTACTAACCAAAAATTAGCATGA", "aa": "MKYVKVQIINKSTIELLEDAKKGEKINLDLINQVDQTNILNTITTNQKLA", "aa_hexdigest": "e403c62e7b38e41ae685bec0fd92ba00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5699.572900000002, "isoelectric_point": 8.034453010559083 }, "id": "KKNAAMEMLJ_361", "locus": "GCA000027325_00325" }, { "type": "cds", "sequence": "contig_1", "start": 187539, "stop": 187793, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATGACAAAGAGCAAGCATTGTTGTTAAAAACCAAATTAGAATTACAAAACCAGTTTCAAAAGCAGATAGAGAACTATATCAATGAGATTAACAAGCTCAAGTTAACCAATAAAGAGCTTGAAATTACTAACCAAAAACAACTAGAAAGCTCACTTAAACTACAGCGAAACGAATTTGAAGAGAAAATCAACCAGCAAAACTTAACGATTGAAAAACTGAAAATTCAACAAGCAAGAAGTAGTATCTGA", "aa": "MLNDKEQALLLKTKLELQNQFQKQIENYINEINKLKLTNKELEITNQKQLESSLKLQRNEFEEKINQQNLTIEKLKIQQARSSI", "aa_hexdigest": "b1080aa3ab92b907a29c4a22b918f316", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10055.436699999993, "isoelectric_point": 8.925728416442873 }, "id": "KKNAAMEMLJ_362", "locus": "GCA000027325_00326" }, { "type": "cds", "sequence": "contig_1", "start": 188007, "stop": 188471, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGTGAGTTTAATGATAGTAAGCATAAATCAAAAAACAAAGATCATATTAGCAAACTGGTCAGGGATGCTAAACGTGCTAACTGCAAGTATGCTTTTTTAATTAGTGAACTTGAACTGGAGACTGAAAATGACATCCAAGTGCGCTTAATGCCAACATTGGAAAGTGGTGTTGAAGTCTATCTAGTTAGACCAATGTTTTTTATCTTAATGCTTAAACTTTTCTATAAGTTAGCTAAGAAGTTGTTTGCCCTTAACCGTTTTCAATCAGTTGAACTAATTGATAAAAATAAGTTAAATGAACAGTTTAAACAGTTGAAAGATAATTTTTTAACCAAAACCTTTTTAGAGATTGAAAAAGTGTGTAAAAGTAACTTAGTTGATATTGAAACACTTGAAAAAGCAGTGGTGAAACTAAGGGTTAGAAACGAACGCGTTTTAGATCAGTTACTTAATAAATGA", "aa": "MKSEFNDSKHKSKNKDHISKLVRDAKRANCKYAFLISELELETENDIQVRLMPTLESGVEVYLVRPMFFILMLKLFYKLAKKLFALNRFQSVELIDKNKLNEQFKQLKDNFLTKTFLEIEKVCKSNLVDIETLEKAVVKLRVRNERVLDQLLNK", "aa_hexdigest": "4ef5e11ec56d49368a7dc844cc105c97", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18255.3119, "isoelectric_point": 9.450823402404787 }, "id": "KKNAAMEMLJ_363", "locus": "GCA000027325_00327" }, { "type": "cds", "sequence": "contig_1", "start": 188607, "stop": 189326, "strand": "+", "frame": 3, "gene": null, "product": "MPN162 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47395", "UniRef:UniRef90_P47395" ], "nt": "ATGAAATTATACCGATCTTTAAAAGCAGCCCTGTTACCAGGGATATGCACTAGCATTTTACTTGCTAGTTGTGCTTCAACAAATACTTATCAAGACCAAAGGAATGCCTTGATTAGTTTGGCTTCTAATCGTGATACTTTAATTGCGAATGCTAAAAAATCCAAAGAAGAAGTGCAAAAAGAAGTTACCAAAATGAATAGTAGTACTTCATCAATGATGACAGCTACCCAAAGTGTTGCAATTACAACACATCAAACTACTGAAAAAACAAATAATTCTAAGTATGATCTAGACAAGCTTTTTAAGGATTACATCCTTTATGTTGTTGATAATTTTTCAGGACTTGTTTTTAAAAGAACTGGCGGTCATAGGATCCAGTTAATCGATAAGGATAAAGAGATTTTGGATGGTGGTAATCTAACTAAACATACCCACCACGATCATAACCATATGCATAATCATGAACATGAACATGAAGAACACCATGATGAGGAAGAGACAGAAGTAGTTGGCAGAGCACTATCTTTTACTAATGGCATCTTTCTAGTGATTGACTATAAAAAAGACTCTGAAAGAAAAAATATGAGTGGTTCAACTACTATGATGCACCAACACCACCATGAAGCTGAAGAACATAAAGAGGAACGTAAGCTCTCTTTAAACTTAAAAGCATACAAATTTAATACCCCTTTTAACATTAGTGAGTTTATTAGTGCTTGA", "aa": "MKLYRSLKAALLPGICTSILLASCASTNTYQDQRNALISLASNRDTLIANAKKSKEEVQKEVTKMNSSTSSMMTATQSVAITTHQTTEKTNNSKYDLDKLFKDYILYVVDNFSGLVFKRTGGHRIQLIDKDKEILDGGNLTKHTHHDHNHMHNHEHEHEEHHDEEETEVVGRALSFTNGIFLVIDYKKDSERKNMSGSTTMMHQHHHEAEEHKEERKLSLNLKAYKFNTPFNISEFISA", "aa_hexdigest": "24b85a02795a7438b94e87d6fd140ede", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47395", "query_cov": 1.0, "subject_cov": 0.8505338078291815, "identity": 1.0, "score": 396.0, "evalue": 1.02e-137, "valid": true, "product": "MPN162 family protein", "uniref50_id": "UniRef50_P47395", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47395", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47395" ], "product": "Uncharacterized lipoprotein MG149" }, "genes": [], "id": "KKNAAMEMLJ_364", "locus": "GCA000027325_00328" }, { "type": "cds", "sequence": "contig_1", "start": 189546, "stop": 189638, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAACAAAACCCTGATCGTTTAAAAAAAGATAGGGAACTTATTTATGCAATTGTTACAGCTAAAGGTATCATTAGCCGTTTCTTTTGA", "aa": "MEQQNPDRLKKDRELIYAIVTAKGIISRFF", "aa_hexdigest": "a2c9ce7421d5ee46c6e43dfc0bd9f152", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3581.1499999999996, "isoelectric_point": 9.518902015686034 }, "id": "KKNAAMEMLJ_365", "locus": "GCA000027325_00329" }, { "type": "cds", "sequence": "contig_1", "start": 189801, "stop": 189956, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_009885834.1", "SO:0001217", "UniParc:UPI0001B417EB", "UniRef:UniRef100_UPI0001B417EB", "UniRef:UniRef50_UPI0001B417EB", "UniRef:UniRef90_UPI0001B417EB" ], "nt": "TTGTTTATCTATTTTAAGGGCCAGAAAGCAATTGAAAACAACTGTAAGTACCTGTTAACAATCCTTGATATTAAGAGTGATGAACTGTTACCTTTTTCGCTTTTAGGTAGTTTAAGAAAAGGTTATATGCTAGATGAAATGCTCTTAGAACAGTAA", "aa": "MFIYFKGQKAIENNCKYLLTILDIKSDELLPFSLLGSLRKGYMLDEMLLEQ", "aa_hexdigest": "9c56816882705e57cdcda1dd5e060d34", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001B417EB", "ncbi_nrp_id": "WP_009885834.1", "uniref100_id": "UniRef100_UPI0001B417EB", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001B417EB", "RefSeq:WP_009885834.1", "UniRef:UniRef100_UPI0001B417EB" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001B417EB", "uniref90_id": "UniRef90_UPI0001B417EB" }, "psc": { "uniref90_id": "UniRef90_UPI0001B417EB", "uniref50_id": "UniRef50_UPI0001B417EB" }, "pscc": { "uniref50_id": "UniRef50_UPI0001B417EB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B417EB" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5988.062999999997, "isoelectric_point": 5.095922660827636 }, "id": "KKNAAMEMLJ_366", "locus": "GCA000027325_00330" }, { "type": "cds", "sequence": "contig_1", "start": 190046, "stop": 190366, "strand": "+", "frame": 2, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627", "RefSeq:WP_009885835.1", "SO:0001217", "UniParc:UPI0000134BB3", "UniRef:UniRef100_P47396", "UniRef:UniRef50_O52331", "UniRef:UniRef90_P75581" ], "nt": "ATGAATAGCGCTGTAAAATATCCTGAGCTGAAGATCAAACTTGAGTCTTATGATAGCACCCTTTTAGATCTCACTATTAAAAAGATAGTTGAGGTTGTAAAGGGTGTGAACATTAAGATTAAAGGTCCTTTACCTTTGCCTACTAAAAAGGAAGTGATCACCATTATCCGCTCTCCCCATGTTGATAAAGCATCCAGAGAGCAGTTTGAAAAAAATACCCACAAGCGCTTAATGATTCTTGTTGATGTTAATCAAGGAGGGATTGATAGTTTAAAAAAGATTAAGATCCCAGTTGGGGTTACACTGCGTTTTTCAAAATAG", "aa": "MNSAVKYPELKIKLESYDSTLLDLTIKKIVEVVKGVNIKIKGPLPLPTKKEVITIIRSPHVDKASREQFEKNTHKRLMILVDVNQGGIDSLKKIKIPVGVTLRFSK", "aa_hexdigest": "81c51a3a40e700b1bc8088cf9449d925", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134BB3", "ncbi_nrp_id": "WP_009885835.1", "uniref100_id": "UniRef100_P47396", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134BB3", "RefSeq:WP_009885835.1", "UniRef:UniRef100_P47396" ] }, "ips": { "uniref100_id": "UniRef100_P47396", "uniref90_id": "UniRef90_P75581" }, "psc": { "uniref90_id": "UniRef90_P75581", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_O52331", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52331", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52331" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "KKNAAMEMLJ_367", "locus": "GCA000027325_00331" }, { "type": "cds", "sequence": "contig_1", "start": 190370, "stop": 190747, "strand": "+", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_P75580", "UniRef:UniRef90_P47397" ], "nt": "ATGGATGTAAGGGGAATATTTGGTGTTAAAGTAGGGATGAGTCAGATCTTTACTGAGCAAAATGAGTGCTTACCTATCACCATTGTTTATTGTGAAGCTAATCAGGTGGCTGGGATTAAAACGATTGCTAAAGATAATTACAACGCCACTCTATTAAGCTTTCAAACTGTTGATGAAAAACAACTTAACAAACCTAAACAAGGGTTCTTTTCCAAACTTAAACTAGAACCTCATAAATATCTGAGGGAAATCAGAAAGATGCAAGGGTTTGAGTTAGGTAAGAAGATCACCCCCCAGGAGTTGTTTAAGATAGGTGAATATGTTGATGTCACTTCACTCACCAAAGGTAGGGGTTTTACAGGAGCGATTAAAAGGTGA", "aa": "MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLEPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKR", "aa_hexdigest": "ab91b20669b8ba3d57eb2675780df1de", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 190370, "stop": 191050, "observations": { "mutation": [ 190745 ], "stop": [ 190745 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47397", "query_cov": 1.0, "subject_cov": 0.48638132295719844, "identity": 1.0, "score": 250.0, "evalue": 2.1e-82, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLEPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTAKKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK" }, "paralog": false, "identity": 0.995575221238938, "score": 448.0, "evalue": 6.23e-163, "description": "Internal stop codon at 190745. Nonsense mutation around 190745.", "psc": { "uniref90_id": "UniRef90_P47397", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_P75580", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47397", "UniRef:UniRef50_P75580", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75580", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75580" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "KKNAAMEMLJ_368", "locus": "GCA000027325_00332" }, { "type": "cds", "sequence": "contig_1", "start": 190766, "stop": 191143, "strand": "+", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_P75580", "UniRef:UniRef90_P47397" ], "nt": "TTGGGTCATGGGGCGGGTTATCCCCACCGCTTTCAGGGTTCTGTGCAAGCAGGTAGAGGTGGTAGTAGTGCGCAGCGTGTTTTTAAGGGTAAGAAGATGTCTGGGCATTATGGTCATGAACAAGTTACGATCCAAAACCTCTTTATTGTTGGCTTTGATGAAATCAATAAGTTAGTGTTAGTTTCAGGCGCAATTGCTGGTCCTGAGGGTGGGATTGTTTTAATTAAAACTGCAAAAAAGAAAACTGGCAAGATAAAAGATATAAAGTTAGCAGTACAAACTGTTAAAGCCCCACAACTAAAAGCACCAAAAAAGCAGAAAACTAAGGTTGAAACCAACCAGGTTAACCCAAAAATTGAAGAAGAGAAAACTAAGTAA", "aa": "MGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTAKKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK", "aa_hexdigest": "ba4659ae07f12691168d010b2341c272", "start_type": "TTG", "rbs_motif": null, "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 190466, "stop": 191143, "observations": { "mutation": [ 190745 ], "stop": [ 190745 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47397", "query_cov": 1.0, "subject_cov": 0.48638132295719844, "identity": 0.992, "score": 233.0, "evalue": 1.45e-75, "gene_start": 1, "gene_end": 125, "reference_start": 133, "reference_end": 257, "reference_sequence": "MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLEPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTAKKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK" }, "paralog": false, "identity": 0.9955555555555555, "score": 443.0, "evalue": 5.94e-161, "description": "Internal stop codon at 190745. Nonsense mutation around 190745.", "psc": { "uniref90_id": "UniRef90_P47397", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_P75580", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47397", "UniRef:UniRef50_P75580", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75580", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75580" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "KKNAAMEMLJ_369", "locus": "GCA000027325_00333" }, { "type": "cds", "sequence": "contig_1", "start": 191143, "stop": 191778, "strand": "+", "frame": 1, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904", "RefSeq:WP_009885836.1", "SO:0001217", "UniParc:UPI00001340F9", "UniRef:UniRef100_P47398", "UniRef:UniRef50_P75579", "UniRef:UniRef90_P47398" ], "nt": "ATGGCTAAACTTAAAGTAATCCAGTTTGATGGTAGTTTTAAAGGTGAGATCCAACCTGCTAACCACCTCCTTTTAAAAAAAGCAGTGATCCAACCAGTGTTTGATGCTATCTTATTAGAACAAGCAGCATGTAGACAAGGCACTCACTCTACTTTAACTAAGGGTGAAGTTAGTGGTGGGGGTAAAAAACCATATAAACAAAAGCACACTGGTAAAGCTAGACAGGGTTCAATAAGAAACCCCCATTATGTGGGGGGTGGTGTTGTTTTTGGTCCTAAACCCAACCGTAACTACAAACTAAAACTAAACAAAAAGGCTTATCAACTTGCTTTAACTAGTGCCTTTGCACAAAAGCTTAACAACAACCAAGTGATAGTTGCTGAAGCCAAGTTGTTTGAACAAACCAATGCCAAAACTAAAAAGATGCTGACGTTTCTCAAGAATGCCAAACTAACTGAGCAAAAACTCTTGTTTGTGATTGATACTATCTCAAAACCACTGTTGTTGAGTACTAACAACCTAAAGCAGATAGTAGTCAAACAGTTTAATAAAGTATCAGTAAGAGATCTACTTTTAGCTAAAACTATCATCATTGAAAAAGCTGCTTTTACAAAACTGGAGGAACGACTTAAATAG", "aa": "MAKLKVIQFDGSFKGEIQPANHLLLKKAVIQPVFDAILLEQAACRQGTHSTLTKGEVSGGGKKPYKQKHTGKARQGSIRNPHYVGGGVVFGPKPNRNYKLKLNKKAYQLALTSAFAQKLNNNQVIVAEAKLFEQTNAKTKKMLTFLKNAKLTEQKLLFVIDTISKPLLLSTNNLKQIVVKQFNKVSVRDLLLAKTIIIEKAAFTKLEERLK", "aa_hexdigest": "4ef0c6907ae54099008de7d7cf4cd805", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001340F9", "ncbi_nrp_id": "WP_009885836.1", "uniref100_id": "UniRef100_P47398", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001340F9", "RefSeq:WP_009885836.1", "UniRef:UniRef100_P47398" ] }, "ips": { "uniref100_id": "UniRef100_P47398", "uniref90_id": "UniRef90_P47398" }, "psc": { "uniref90_id": "UniRef90_P47398", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_P75579", "cog_id": "COG0088", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P75579", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75579" ], "product": "Large ribosomal subunit protein uL4" }, "genes": [ "rplD" ], "id": "KKNAAMEMLJ_370", "locus": "GCA000027325_00334" }, { "type": "cds", "sequence": "contig_1", "start": 191782, "stop": 192102, "strand": "+", "frame": 1, "gene": "rplW", "product": "50S ribosomal protein L23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_009885837.1", "SO:0001217", "UniParc:UPI0000133DA2", "UniRef:UniRef100_P47399", "UniRef:UniRef50_P47399", "UniRef:UniRef90_P47399" ], "nt": "ATGGATGTAACCAACATACTCTTAAAACCAGTCTTAACTGAAAAGAGTTATCTCAACCAGATGGGGGAATTGAAAAAATATGTCTTTGCAATTAACCCTAAAGCTACTAAAACCAAAGTAAAACTAGCGTTTGAAATTATCTATGGGGTTAAACCTTTAAAGATTAACACGCTAATTAGAAAACCAGTGACCATTAGAAATGGCACTAAATACCCTGGGTTTAGTAAGCTAGCAAAACTAGCAGTAATCACCTTACCTAAGGGAATGGATATTGCCATTACTGGTGAGAAAACAACCAAGAAAGAAACAAAGGATCAATAA", "aa": "MDVTNILLKPVLTEKSYLNQMGELKKYVFAINPKATKTKVKLAFEIIYGVKPLKINTLIRKPVTIRNGTKYPGFSKLAKLAVITLPKGMDIAITGEKTTKKETKDQ", "aa_hexdigest": "cb3bde973f635e2c137d7725dd7ff6fe", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DA2", "ncbi_nrp_id": "WP_009885837.1", "uniref100_id": "UniRef100_P47399", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DA2", "RefSeq:WP_009885837.1", "UniRef:UniRef100_P47399" ] }, "ips": { "uniref100_id": "UniRef100_P47399", "uniref90_id": "UniRef90_P47399" }, "psc": { "uniref90_id": "UniRef90_P47399", "gene": "rplW", "product": "50S ribosomal protein L23", "uniref50_id": "UniRef50_P47399", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:1990904", "GO:0005840", "GO:0019843", "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P47399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47399" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "KKNAAMEMLJ_371", "locus": "GCA000027325_00335" }, { "type": "cds", "sequence": "contig_1", "start": 192102, "stop": 192959, "strand": "+", "frame": 3, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "COG:COG0090", "COG:J", "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843", "GO:0022625", "KEGG:K02886", "RefSeq:WP_009885838.1", "SO:0001217", "UniParc:UPI0000133ECD", "UniRef:UniRef100_P47400", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_P47400" ], "nt": "ATGGCAATTAAAAAGATTATTAGTCGTTCTAACAGTGGGATTCACAACGCCACTGTCATTGACTTTAAAAAACTCCTTACCAATTCCAAACCCGAAAAGTCGCTTTTAGTTACTTTAAAAAAACATGCAGGAAGAAACAACCAGGGCAAGATCACTGTTCGCCACCACGGTGGGAGACATAAACGTAAGTACCGTTTAATTGATTTTAAGCGTTACCACTATGACAATTTAAAAGCAACTGTTAAATCGATTGAATATGATCCTAACCGCAGTTGTTTTATCTCCCTTTTACACTATCAGAATGGGGTTAAAACTTACATCATTAGTCCTGATGGGATTAAGGTTGGTGATCAAGTTTATTCATCTGATCATGCCATTGATATCAAACTAGGTTATTGTATGCCCCTTGCTTTTATCCCTGAAGGAACCCAAGTTCATAACATTGAACTTAACCCTAAGGGTGGGGGTAAGATAGCAAGAAGTGCTGGAAGTTATGCGAGGATCTTGGGTCAAGATGAGACTGGTAAATACATCATTCTCCAGTTAATCTCAGGGGAAACTAGGAAGTTTTTAAAGGAGTGTAGAGCTACAGTTGGTGTTGTCTCTAACTTAGATCATAACCTTGTTGTAATTGGTAAAGCAGGGAGAAGTCGTCATAAGGGAATCAGACCAACGGTTAGAGGTTCAGCAATGAACCCTAATGACCACCCGCATGGGGGTGGGGAAGGGAGAAGCCCAGTTGGCAGAGATGCACCAAGAACCCCTTGGGGCAAACGCCATATGGGTGTGAAAACACGTAACATGAAAAAACATTCAACTAACCTGATTATTAGAAACAGAAAAGGAGAACAATACTAA", "aa": "MAIKKIISRSNSGIHNATVIDFKKLLTNSKPEKSLLVTLKKHAGRNNQGKITVRHHGGRHKRKYRLIDFKRYHYDNLKATVKSIEYDPNRSCFISLLHYQNGVKTYIISPDGIKVGDQVYSSDHAIDIKLGYCMPLAFIPEGTQVHNIELNPKGGGKIARSAGSYARILGQDETGKYIILQLISGETRKFLKECRATVGVVSNLDHNLVVIGKAGRSRHKGIRPTVRGSAMNPNDHPHGGGEGRSPVGRDAPRTPWGKRHMGVKTRNMKKHSTNLIIRNRKGEQY", "aa_hexdigest": "b29c106011d890d3aa3a4e50207ce901", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133ECD", "ncbi_nrp_id": "WP_009885838.1", "uniref100_id": "UniRef100_P47400", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133ECD", "RefSeq:WP_009885838.1", "UniRef:UniRef100_P47400" ] }, "ips": { "uniref100_id": "UniRef100_P47400", "uniref90_id": "UniRef90_P47400" }, "psc": { "uniref90_id": "UniRef90_P47400", "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "cog_id": "COG0090", "cog_category": "J", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "KKNAAMEMLJ_372", "locus": "GCA000027325_00336" }, { "type": "cds", "sequence": "contig_1", "start": 192959, "stop": 193222, "strand": "+", "frame": 2, "gene": "rpsS", "product": "30S ribosomal protein S19", "db_xrefs": [ "COG:COG0185", "COG:J", "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "RefSeq:WP_009885839.1", "SO:0001217", "UniParc:UPI0000134DEA", "UniRef:UniRef100_P47401", "UniRef:UniRef50_Q6ME59", "UniRef:UniRef90_P75576" ], "nt": "ATGTCAAGAAGTAGTAAAAAGGGCGCATTTGTTGATGCTCACCTCTTAAAAAAAGTGATTGAAATGAACAAACAAGCCAAGAAAAAACCAATTAAGACTTGGTCAAGAAGAAGTACTATCTTCCCTGAGTTTGTGGGTAACACCTTCAGTGTGCATAACGGTAAAACCTTTATTAATGTTTATGTTACTGATGATATGGTAGGTCATAAGTTGGGTGAGTTTTCCCCAACTAGAAACTTTAAACAACACACTGCTAACCGTTAG", "aa": "MSRSSKKGAFVDAHLLKKVIEMNKQAKKKPIKTWSRRSTIFPEFVGNTFSVHNGKTFINVYVTDDMVGHKLGEFSPTRNFKQHTANR", "aa_hexdigest": "3f214f53aa7f11b4874ded3835d887c3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134DEA", "ncbi_nrp_id": "WP_009885839.1", "uniref100_id": "UniRef100_P47401", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DEA", "RefSeq:WP_009885839.1", "UniRef:UniRef100_P47401" ] }, "ips": { "uniref100_id": "UniRef100_P47401", "uniref90_id": "UniRef90_P75576" }, "psc": { "uniref90_id": "UniRef90_P75576", "gene": "rpsS", "product": "30S ribosomal protein S19", "uniref50_id": "UniRef50_Q6ME59", "cog_id": "COG0185", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q6ME59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6ME59" ], "product": "Small ribosomal subunit protein uS19" }, "genes": [ "rpsS" ], "id": "KKNAAMEMLJ_373", "locus": "GCA000027325_00337" }, { "type": "cds", "sequence": "contig_1", "start": 193225, "stop": 193659, "strand": "+", "frame": 1, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "COG:COG0091", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02890", "RefSeq:WP_009885840.1", "SO:0001217", "UniParc:UPI0000133D78", "UniRef:UniRef100_P47402", "UniRef:UniRef50_P47402", "UniRef:UniRef90_P47402" ], "nt": "ATGATTGCTTTTGCTAAACAATACAGAGTTCACATCTCCCCCCAAAAAGCACGGTTAGTGTGCCAGTTAATTGTGGGTAAGAAGATTAATGATGCGCAAAACATCCTTTTAAATACGCCAAAGAAAGCTGCTTACTTTTTAACTAAGTTACTAAATAGTGCGATTAGTAATGCCACTAATAACCACGGGATGAGCGGGGATCTTTTGTATGTATTTGAATGTGTTGCTAACCAAGGACCTAGCATGAAAAGAACAATCGCTAGAGCCAAAGGTTCAGGGAGTGTTTTAACCAAGCGTTCTTCAAACCTAGTTATTAAGTTATCTGATAATCCCAATGAAAGAAAATTACTCTTAACCCAACAAAAGGAACTGGTGAAAAAAAGAACAATGGGTCATAAAAAAGAGAAAGCAAAGCAAAAGCAAAAACAACAATAA", "aa": "MIAFAKQYRVHISPQKARLVCQLIVGKKINDAQNILLNTPKKAAYFLTKLLNSAISNATNNHGMSGDLLYVFECVANQGPSMKRTIARAKGSGSVLTKRSSNLVIKLSDNPNERKLLLTQQKELVKKRTMGHKKEKAKQKQKQQ", "aa_hexdigest": "5ef95273909311097b2a84d0940b84a8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D78", "ncbi_nrp_id": "WP_009885840.1", "uniref100_id": "UniRef100_P47402", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D78", "RefSeq:WP_009885840.1", "UniRef:UniRef100_P47402" ] }, "ips": { "uniref100_id": "UniRef100_P47402", "uniref90_id": "UniRef90_P47402" }, "psc": { "uniref90_id": "UniRef90_P47402", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_P47402", "cog_id": "COG0091", "cog_category": "J", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47402" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "KKNAAMEMLJ_374", "locus": "GCA000027325_00338" }, { "type": "cds", "sequence": "contig_1", "start": 193818, "stop": 194468, "strand": "+", "frame": 3, "gene": "rpsC", "product": "30S ribosomal protein S3", "db_xrefs": [ "COG:COG0092", "COG:J", "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627", "KEGG:K02982", "SO:0001217", "UniRef:UniRef50_O52338", "UniRef:UniRef90_P47403" ], "nt": "GTGTCTAATGTTGAGATAGAAAGAACCCAAACGACTGTTGATGTTTATGTCTATGCAGCTCAACCTGCTTTATTGATAGGCAGTGAAAACAAAAACATCCAAAAGATTACCAAAATGATCCAAATCATTGTGGGCAGAAAGATTAAACTTGATCTTACTATCAATGAGATCGGCTCTCCGATGTTATCAAGTAGGATCATTGCCCGTGATATTGCTAATGCGATTGAAAACAGAGTACCACTCCGTTCAGCAATGCGCCAAGCTCTAACCAAGGTTTTAAAAGCAGGTGCTAATGGGATTAAGGTATTGGTATCAGGCAGATTAAATGGGGCGGAAATTGCCCGTGACAAGATGTATATTGAGGGCAATATGCCTCTTTCAACTTTAAGAGCAGATATTGACTATGCCTTTGAAAAAGCAAAAACCACCTATGGCATTATTGGGGTGAAAGTATGGATTAACAGGGGGATGATCTATGCAAAGGGTTTAAACAGAACCCCAGCACACATCCTCCATCCCCAAAAGAAACAGCTAAAAACCCCAACTATCAAAAAAACCAATTCAGTAATAGCAAAACAAAAACTCACTGGTAGTGATATTGAAACTGCTAGTTTAAAAGCACTTACTGATAATAATCAAAACCACGAATAG", "aa": "MSNVEIERTQTTVDVYVYAAQPALLIGSENKNIQKITKMIQIIVGRKIKLDLTINEIGSPMLSSRIIARDIANAIENRVPLRSAMRQALTKVLKAGANGIKVLVSGRLNGAEIARDKMYIEGNMPLSTLRADIDYAFEKAKTTYGIIGVKVWINRGMIYAKGLNRTPAHILHPQKKQLKTPTIKKTNSVIAKQKLTGSDIETASLKALTDNNQNHE", "aa_hexdigest": "44fb446fc3d65b5806df197fd2afc470", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47403", "query_cov": 1.0, "subject_cov": 0.8059701492537313, "identity": 0.995, "score": 367.0, "evalue": 1.36e-126, "valid": true, "gene": "rpsC", "product": "30S ribosomal protein S3", "uniref50_id": "UniRef50_O52338", "cog_id": "COG0092", "cog_category": "J", "kegg_orthology_id": "K02982", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52338" ], "product": "Small ribosomal subunit protein uS3" }, "genes": [ "rpsC" ], "id": "KKNAAMEMLJ_375", "locus": "GCA000027325_00339" }, { "type": "cds", "sequence": "contig_1", "start": 194474, "stop": 194668, "strand": "+", "frame": 2, "gene": "rplP", "product": "50S ribosomal protein L16", "db_xrefs": [ "COG:COG0197", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02878", "SO:0001217", "UniRef:UniRef50_P02415", "UniRef:UniRef90_P47404" ], "nt": "ATGTTACAACCAAAAAGAACCAAATACAGAAAACCACATAACGTCAGTTATGAAGGACACACTAAGGGCAATGGTTATGTTGCTTTTGGTGAGTATGGAATTGTTGCTACTAAGGGTAATTGGATCGATGCGAGAGCAATTGAATCAGCGCGGGTTGCTATCTCAAAGTGCTTGGGTAAAACTGGAAAGATGTGA", "aa": "MLQPKRTKYRKPHNVSYEGHTKGNGYVAFGEYGIVATKGNWIDARAIESARVAISKCLGKTGKM", "aa_hexdigest": "b9d8b1d6fece06001424e9d7004b3a9a", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplP" ], "truncated": "3-prime", "pseudogene": { "start": 194474, "stop": 194890, "observations": { "mutation": [ 194666, 194858 ], "stop": [ 194666, 194858 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47404", "query_cov": 1.0, "subject_cov": 0.460431654676259, "identity": 0.922, "score": 124.0, "evalue": 4.4e-35, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MLQPKRTKYRKPHNVSYEGKAKGNSYVAFGEYGLVATKGNWIDARAIESARIAISKCLGKTGKMWIRIFPHMSKTKKPLEVRMGSGKGNPEFWVAVVKQGTVMFEVANIPESQMIKALTRAGHKLPVTWKILKREEVSA" }, "paralog": false, "identity": 0.9130434782608695, "score": 258.0, "evalue": 1.43e-90, "description": "Internal stop codon at 194666, 194858. Nonsense mutation around 194666, 194858.", "psc": { "uniref90_id": "UniRef90_P47404", "gene": "rplP", "product": "50S ribosomal protein L16", "uniref50_id": "UniRef50_P02415", "cog_id": "COG0197", "cog_category": "J", "kegg_orthology_id": "K02878", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47404", "UniRef:UniRef50_P02415", "COG:COG0197", "COG:J", "KEGG:K02878", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P02415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P02415" ], "product": "Large ribosomal subunit protein uL16" } }, "id": "KKNAAMEMLJ_376", "locus": "GCA000027325_00340" }, { "type": "cds", "sequence": "contig_1", "start": 194687, "stop": 194860, "strand": "+", "frame": 2, "gene": null, "product": "Large ribosomal subunit protein uL16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_M5SUM2" ], "nt": "ATGTCAAAAACCAAAAAACCCTTAGAAGTGAGGATGGGTTCAGGGAAAGGTAACCCTGAATTTTGGGTTGCTGTTGTTAAAAAGGGGACAGTGATGTTTGAAGTTGCTAACATCCCTGAACAACAGATGATCAAAGCCTTAACAAGAGCAGGCCATAAACTCCCTGTTACCTGA", "aa": "MSKTKKPLEVRMGSGKGNPEFWVAVVKKGTVMFEVANIPEQQMIKALTRAGHKLPVT", "aa_hexdigest": "40ea8c93f2f0ea231b9d0e2882bcb9b2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_M5SUM2", "query_cov": 0.9298245614035088, "subject_cov": 0.828125, "identity": 0.509, "score": 63.9, "evalue": 2.35e-12, "valid": false, "product": "Large ribosomal subunit protein uL16", "uniref50_id": "UniRef50_M5SUM2" }, "pscc": { "uniref50_id": "UniRef50_M5SUM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_M5SUM2" ], "product": "Large ribosomal subunit protein uL16" }, "genes": [], "id": "KKNAAMEMLJ_377", "locus": "GCA000027325_00341" }, { "type": "cds", "sequence": "contig_1", "start": 194890, "stop": 195084, "strand": "+", "frame": 1, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_UPI0029620CC7", "UniRef:UniRef90_UPI0029620CC7" ], "nt": "ATGACAATCGCTAAGGAGCTGAAGCAAAAGAGCAACGAAGAGTTAGTGAAACTAGTAATTAAGCTTAAGGGTGAACTCTTAGAATACCGCTTTAAACTTGCCCATGGTGAACTTGACAAACCCCATCTGATTGCCAAGGTGAGAAAGTTATTAGCAGTTGTACTTACTATTCTCACTGAACGCAAACTCAACTGA", "aa": "MTIAKELKQKSNEELVKLVIKLKGELLEYRFKLAHGELDKPHLIAKVRKLLAVVLTILTERKLN", "aa_hexdigest": "2b3b8b6dc7f453cfcd94f3666e7c3513", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpmC" ], "truncated": "3-prime", "pseudogene": { "start": 194896, "stop": 195204, "observations": { "mutation": [ 195082, 195148 ], "stop": [ 195082, 195148 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029620CC7", "query_cov": 0.96875, "subject_cov": 0.4460431654676259, "identity": 0.968, "score": 111.0, "evalue": 3.23e-30, "gene_start": 3, "gene_end": 64, "reference_start": 1, "reference_end": 62, "reference_sequence": "MAKELKQKSNEELVKLVIKLKGELLEYRFKLAHGELDKPHLIAKVRKLLAVVLTILTERKINWQVEKDKYKLLSRKTNELIVNSWKQKLSTKPESKQETKKAEVKPKVEPKPLKQETKKVEENKKLKRLKLNLKLNQNL" }, "paralog": false, "identity": 0.9607843137254902, "score": 185.0, "evalue": 6.68e-62, "description": "Internal stop codon at 195082, 195148. Nonsense mutation around 195082, 195148.", "psc": { "uniref90_id": "UniRef90_UPI0029620CC7", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_UPI0029620CC7", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029620CC7", "UniRef:UniRef50_UPI0029620CC7", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029620CC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029620CC7" ], "product": "50S ribosomal protein L29" } }, "id": "KKNAAMEMLJ_378", "locus": "GCA000027325_00342" }, { "type": "cds", "sequence": "contig_1", "start": 195492, "stop": 195716, "strand": "+", "frame": 3, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "SO:0001217", "UniRef:UniRef50_P47406", "UniRef:UniRef90_P47406" ], "nt": "ATGAAGCGCAACCAACGTAAGCAGTTAATTGGCACAGTTGTTAGCACCAAAAATGCTAAAACAGCAACTGTCAAAGTAACATCACGCTTTAAACATCCTTTGTATCACAAATCAGTTATTCGCCATAAAAAGTACCATGTCCATAACTTTGGTGAACTTGTTGCTAATGATGGTGATAGGGTACAAATTATTGAAACAAGACCCCTTTCCGCTTTAAAGCGGTGA", "aa": "MKRNQRKQLIGTVVSTKNAKTATVKVTSRFKHPLYHKSVIRHKKYHVHNFGELVANDGDRVQIIETRPLSALKR", "aa_hexdigest": "0ccbd0b07373a87d1ad21f01e9db1014", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47406", "query_cov": 1.0, "subject_cov": 0.8705882352941177, "identity": 1.0, "score": 144.0, "evalue": 7.83e-44, "valid": true, "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_P47406", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47406" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "KKNAAMEMLJ_379", "locus": "GCA000027325_00343" }, { "type": "cds", "sequence": "contig_1", "start": 195753, "stop": 196121, "strand": "+", "frame": 3, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02874", "RefSeq:WP_009885845.1", "SO:0001217", "UniParc:UPI0000133BE7", "UniRef:UniRef100_P47407", "UniRef:UniRef50_Q5SHP8", "UniRef:UniRef90_P47407" ], "nt": "ATGGTTAGTTTTATGACAAGATTAAATGTAGCTGATAATACAGGCGCTAAGCAAGTAGGTATTATCAAAGTTTTAGGTGCTACATACAAACGTTATGCATTCCTTGGTGATGTTGTTGTTGTATCAGTTAAAGATGCAATCCCTAATGGCATGGTTAAAAAGGGTCAAGTGTTAAGAGCAGTCATTGTTAGAACCAAAAAGGGACAACAACGCCAAGATGGTACCCACCTAAAGTTCCATGACAATGCTTGTGTGCTTATCAAAGAAGATAAATCCCCAAGGGGAACAAGAATCTTTGGACCAGTTGCTAGAGAGTTGAGAGAAAAAGGTTACAACAAGATTTTAAGCTTGGCGGTGGAGGTTGTTTAA", "aa": "MVSFMTRLNVADNTGAKQVGIIKVLGATYKRYAFLGDVVVVSVKDAIPNGMVKKGQVLRAVIVRTKKGQQRQDGTHLKFHDNACVLIKEDKSPRGTRIFGPVARELREKGYNKILSLAVEVV", "aa_hexdigest": "a3dba71f87df18733f7f3013a4c99584", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133BE7", "ncbi_nrp_id": "WP_009885845.1", "uniref100_id": "UniRef100_P47407", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133BE7", "RefSeq:WP_009885845.1", "UniRef:UniRef100_P47407" ] }, "ips": { "uniref100_id": "UniRef100_P47407", "uniref90_id": "UniRef90_P47407" }, "psc": { "uniref90_id": "UniRef90_P47407", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_Q5SHP8", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHP8" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "KKNAAMEMLJ_380", "locus": "GCA000027325_00344" }, { "type": "cds", "sequence": "contig_1", "start": 196121, "stop": 196447, "strand": "+", "frame": 2, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "RefSeq:WP_009885846.1", "SO:0001217", "UniParc:UPI0000133DCB", "UniRef:UniRef100_P47408", "UniRef:UniRef50_O52343", "UniRef:UniRef90_P47408" ], "nt": "ATGCAAAGGATTAGAAAAGGTGATAAGGTAGTTGTGATCACTGGTAAAAACAAGGGTGGTAGTGGGATAGTGCTTAAGGTATTAACCAAGCAAAACAAAGCGATTGTTGAGGGGATCAATAAGGTTACTGTTCACAAAAAAGAACAAGTCAACAAGCGCAGCAAACAAACAAACCCAACTACTAAAGAAGCCCCTTTACCATTAAATAAACTTGCTTTATTTGATCAGAAGGCCAAACAGCAAACAATTGGCAAGATCAAATACCAAATTGATCCTAAAACCAAACAAAAAACAAGAGTCTTTAAGAAGACTAATAATGCCATTTAA", "aa": "MQRIRKGDKVVVITGKNKGGSGIVLKVLTKQNKAIVEGINKVTVHKKEQVNKRSKQTNPTTKEAPLPLNKLALFDQKAKQQTIGKIKYQIDPKTKQKTRVFKKTNNAI", "aa_hexdigest": "9735561e3d016a8dbea25d505dd52901", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DCB", "ncbi_nrp_id": "WP_009885846.1", "uniref100_id": "UniRef100_P47408", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DCB", "RefSeq:WP_009885846.1", "UniRef:UniRef100_P47408" ] }, "ips": { "uniref100_id": "UniRef100_P47408", "uniref90_id": "UniRef90_P47408" }, "psc": { "uniref90_id": "UniRef90_P47408", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_O52343", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_O52343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52343" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "KKNAAMEMLJ_381", "locus": "GCA000027325_00345" }, { "type": "cds", "sequence": "contig_1", "start": 196453, "stop": 196743, "strand": "+", "frame": 1, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380ENH6" ], "nt": "ATGAATAACCTTGAAAAAACCTATAAAACTGAGTTAGTTAATCAACTCCAACAACAGTTGGGCTTTTCTTCCATTATGCAAGTCCCTAAGTTAACAAAAATCGTTGTTAACATGGGAGTTGGGGATGCAATTAGAGACAACAAGTTCCTTGAATCAGCACTAAATGAACTGCACCTGATTACTGGTCAAAAACCCGTTGCTACTAAAGCTAAGAATGCTATCTCAACTTACAAGTTACGTGCTGGCCAATTAATTGGTTGTAAAGTTACTCTAAGAAATAAAAAGATGTGA", "aa": "MNNLEKTYKTELVNQLQQQLGFSSIMQVPKLTKIVVNMGVGDAIRDNKFLESALNELHLITGQKPVATKAKNAISTYKLRAGQLIGCKVTLRNKKM", "aa_hexdigest": "041c65435d155804689965cccf73494f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380ENH6", "query_cov": 0.9791666666666666, "subject_cov": 0.9494949494949495, "identity": 0.5429999999999999, "score": 108.0, "evalue": 6.19e-29, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A380ENH6", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A380ENH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380ENH6" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "KKNAAMEMLJ_382", "locus": "GCA000027325_00346" }, { "type": "cds", "sequence": "contig_1", "start": 196998, "stop": 197180, "strand": "+", "frame": 3, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "COG:COG0199", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_P12878", "UniRef:UniRef90_P47410" ], "nt": "ATGGCTAAAAAATCATTAAAAGTAAAACAATCCCGTCCCAATAAGTTTAGTGTACGCGACTACACCAGGTGTTTAAGGTGTGGGCGTGCTAGAGCAGTGTTAAGCCACTTTGGTGTGTGTAGGTTGTGTTTCCGTGAACTTGCTTATGCAGGAGCAATCCCAGGAGTTAAAAAAGCATCATGA", "aa": "MAKKSLKVKQSRPNKFSVRDYTRCLRCGRARAVLSHFGVCRLCFRELAYAGAIPGVKKAS", "aa_hexdigest": "985e1a9c2861017fe85276491b751686", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47410", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 1.0, "score": 120.0, "evalue": 6.41e-35, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_P12878", "cog_id": "COG0199", "cog_category": "J", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P12878", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P12878" ], "product": "Small ribosomal subunit protein uS14B" }, "genes": [ "rpsZ" ], "id": "KKNAAMEMLJ_383", "locus": "GCA000027325_00347" }, { "type": "cds", "sequence": "contig_1", "start": 197177, "stop": 197602, "strand": "+", "frame": 2, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_010869359.1", "SO:0001217", "UniParc:UPI000013516D", "UniRef:UniRef100_P47411", "UniRef:UniRef50_Q9PQP6", "UniRef:UniRef90_P47411" ], "nt": "ATGATAATCAATAAAGTTCCCAAAGCCCATTTTGATCCAGTTTCTGATCTTTTCACTAAGATCAACAATGCTAGAAAAGCTAAGCTTTTAACTGTTACCACCATCGCTTCTAAGTTAAAGATAGCTATCTTAGAGATTTTGATTAAAGAGGGCTATTTAGCTAACTATCAGGTGTTGGAAAATAAAACTAAAACCAAAAAACTAGTTAGTTTCACATTAAAATACACCCAAAGAAGGATATGTTCTATTAATGGGGTGAAACAGATCTCAAAACCAGGATTAAGAATCTATCGTTCCTTTGAAAAACTTCCCCTTGTTTTAAATGGTCTTGGTATTGCAATTATCTCCACTAGTGATGGAGTGATGACTGATAAAGTAGCAAGGTTAAAGAAGATTGGTGGGGAGATTTTAGCTTACGTTTGGTAA", "aa": "MIINKVPKAHFDPVSDLFTKINNARKAKLLTVTTIASKLKIAILEILIKEGYLANYQVLENKTKTKKLVSFTLKYTQRRICSINGVKQISKPGLRIYRSFEKLPLVLNGLGIAIISTSDGVMTDKVARLKKIGGEILAYVW", "aa_hexdigest": "d93016cb3156fd323ac12380b7f7223e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013516D", "ncbi_nrp_id": "WP_010869359.1", "uniref100_id": "UniRef100_P47411", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013516D", "RefSeq:WP_010869359.1", "UniRef:UniRef100_P47411" ] }, "ips": { "uniref100_id": "UniRef100_P47411", "uniref90_id": "UniRef90_P47411" }, "psc": { "uniref90_id": "UniRef90_P47411", "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q9PQP6", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP6" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "KKNAAMEMLJ_384", "locus": "GCA000027325_00348" }, { "type": "cds", "sequence": "contig_1", "start": 197609, "stop": 198070, "strand": "+", "frame": 2, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "SO:0001217", "UniRef:UniRef50_P47412", "UniRef:UniRef90_P47412" ], "nt": "ATGTCAAAAATAGGAAATAGATCAATCAAAATTGATCCTAGTAAAGTGAGTTTAATGCAAACAACAACACTGCTTACTATTAAAGGACCATTAGGGGAAAACACCATTAAACTACCCAAAAACTTACCCTTAAAGTTTGTTGTTGAAAATGACACTATTAAAGTAACTAATAACAACAACTTAAAACAAACTAAGATCTTACACGGTACTTTCAATGCGTTAGTTAACAACGCAGTTATTGGGGTTACCAAGGGTTTTGAAAAGAAACTCATCCTAGTTGGGGTTGGTTATCGTGCTAATGTGGAAGGGCAATTTCTCAACTTACAATTGGGCTATTCCCATCCTATTAAGGAGTTGATCCCAAACCAACTTACTGTTAAAGTAGAGAAGAACACTGAAATCACCATTAGTGGAATAAAAAAAGAGTTAGTAGGTCAGTTTGCCACTGAAATCAGAAAGTGA", "aa": "MSKIGNRSIKIDPSKVSLMQTTTLLTIKGPLGENTIKLPKNLPLKFVVENDTIKVTNNNNLKQTKILHGTFNALVNNAVIGVTKGFEKKLILVGVGYRANVEGQFLNLQLGYSHPIKELIPNQLTVKVEKNTEITISGIKKELVGQFATEIRK", "aa_hexdigest": "f2fd87274738f6e25dad6fb7a38c7f4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47412", "query_cov": 1.0, "subject_cov": 0.8315217391304348, "identity": 1.0, "score": 297.0, "evalue": 2.13e-101, "valid": true, "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_P47412", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47412" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "KKNAAMEMLJ_385", "locus": "GCA000027325_00349" }, { "type": "cds", "sequence": "contig_1", "start": 198123, "stop": 198512, "strand": "+", "frame": 3, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625", "SO:0001217", "UniRef:UniRef50_P47413", "UniRef:UniRef90_P47413" ], "nt": "TTGTTAGAAAACAAGGTAAAACTGCAGAGGGCAAGAAATAAGATGACAAGAAACGATAAAAGAAGGATTAGACACAAACGGATTGTCAAAAAGATTAGGTTAACTAACCTTAACAACAGGGTTGTACTAATTGTTATCAAGAGTTTAAAAAACATCTCGGTTCAAGCTTGGGACTTTAGTAAGAACGTTGTTTTAACATCAAGTTCCTCACTTCAACTAAAATTAAAAAATGGCAACAAGGAGAATGCTAAACTAGTGGGAATGGATATTGCAACCAAACTCATCAAACTAAACCAAAAGGATGTGGTTTTTGATACTGGGGGTAGTAAGTACCATGGTAGGATTGCTGCTTTAGCAGAAGGAGCGCGAGCTAAGGGTTTAAATTTTTAA", "aa": "MLENKVKLQRARNKMTRNDKRRIRHKRIVKKIRLTNLNNRVVLIVIKSLKNISVQAWDFSKNVVLTSSSSLQLKLKNGNKENAKLVGMDIATKLIKLNQKDVVFDTGGSKYHGRIAALAEGARAKGLNF", "aa_hexdigest": "15e9ca914fcccb25399aefec1d6ff1b5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47413", "query_cov": 0.8914728682170543, "subject_cov": 1.0, "identity": 1.0, "score": 212.0, "evalue": 2.73e-69, "valid": true, "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_P47413", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47413", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47413" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "KKNAAMEMLJ_386", "locus": "GCA000027325_00350" }, { "type": "cds", "sequence": "contig_1", "start": 198517, "stop": 199152, "strand": "+", "frame": 1, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "COG:COG0098", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "KEGG:K02988", "RefSeq:WP_009885854.1", "SO:0001217", "UniParc:UPI000013505F", "UniRef:UniRef100_P47414", "UniRef:UniRef50_Q50301", "UniRef:UniRef90_P47414" ], "nt": "ATGAATGATCAAAAAACTACTAACACTGGCTTGTTAACTTCCACTCTTAAAACCAAGCCCAAACACAACCTTAAACCTTCCAGTGAAGCCATTAAAAAAGCAGTGTCCAAAAAGGAAGGTCATTACAAAAACAAGCGCTTTCAAAAACATAACTTTAATAACAAAAGTGAGTTTGAAGAGAGGATTGTCAAACTCAAACGGATCTCCAAAACCACAAAAGGTGGGAGAAACATGCGCTTTAGTGTCCTTGTTGTTGTTGGTAACAAAAAGGGCAAGGTTGGTTATGGGATTGCTAAGGCATTGGAAGTACCACTTGCCATTAAAAAAGCGATTAAAAAAGCCCATAACTCCATTCATACAGTAGAGATCCATAAGGGTTCAATCTACCACGAAGTGATTGGTAGAAAAGGTGCATCTAAGGTGTTGTTAAAACCTGCACCTTTAGGAACTGGGATCATTGCTGGGGGAGCGATCCGTGCAATTGTAGAGTTAGCTGGTTTTAGTGATATCTATACCAAGAACTTGGGAAGAAACACCCCCATTAACATGATCCATGCCACTATGGATGGGATCTTAAAGCAACTCTCACCCAAAAAAGTGGCATTATTAAGAAATAAACCAATTAGTGATCTATAA", "aa": "MNDQKTTNTGLLTSTLKTKPKHNLKPSSEAIKKAVSKKEGHYKNKRFQKHNFNNKSEFEERIVKLKRISKTTKGGRNMRFSVLVVVGNKKGKVGYGIAKALEVPLAIKKAIKKAHNSIHTVEIHKGSIYHEVIGRKGASKVLLKPAPLGTGIIAGGAIRAIVELAGFSDIYTKNLGRNTPINMIHATMDGILKQLSPKKVALLRNKPISDL", "aa_hexdigest": "7e209050f963e4e037ea509a6ecbdd6c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013505F", "ncbi_nrp_id": "WP_009885854.1", "uniref100_id": "UniRef100_P47414", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013505F", "RefSeq:WP_009885854.1", "UniRef:UniRef100_P47414" ] }, "ips": { "uniref100_id": "UniRef100_P47414", "uniref90_id": "UniRef90_P47414" }, "psc": { "uniref90_id": "UniRef90_P47414", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_Q50301", "cog_id": "COG0098", "cog_category": "J", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q50301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50301" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "KKNAAMEMLJ_387", "locus": "GCA000027325_00351" }, { "type": "cds", "sequence": "contig_1", "start": 199158, "stop": 199610, "strand": "+", "frame": 3, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02876", "RefSeq:WP_010869361.1", "SO:0001217", "UniParc:UPI0000133C15", "UniRef:UniRef100_P47415", "UniRef:UniRef50_P47415", "UniRef:UniRef90_P47415" ], "nt": "ATGGAACTACACCAATTAAAAAGTGTCTCTAAAAGCCGTAACCACAAGTCCAAAGTGGTAGGTAGGGGCCATGGCTCGGGATTAGGTAAAACATCATCACGTGGTCAAAAGGGACAAAAAGCAAGAAAATCAGGTTTAACTAGGTTAGGTTTTGAAGGGGGACAAACACCCCTTTACCGCCGGTTGCCTAAGTATGGGGTTGCTAACAAAGGGATCTTAAAAAAAAGGTGGGTTGTTTTAAATTTGAACAAAGTTGCTAAACTCAATCTCAAAACAGTTACTAGAGCAACTTTGATTGAAAAAAAGGTAATTAGTAAAAAAAATAACCTCCCTTTGAAGTTAATTGGGAACACAAAACTCACTACTCCCATCCACTTTGAAGTGCAAAAAATCTCCAAAAATGCTTTAAATGCAGTGCAAACTAGCAAAGGTAGTGTGAAAATTATCACCTAA", "aa": "MELHQLKSVSKSRNHKSKVVGRGHGSGLGKTSSRGQKGQKARKSGLTRLGFEGGQTPLYRRLPKYGVANKGILKKRWVVLNLNKVAKLNLKTVTRATLIEKKVISKKNNLPLKLIGNTKLTTPIHFEVQKISKNALNAVQTSKGSVKIIT", "aa_hexdigest": "4c3312aadfabef538e5148365a549821", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133C15", "ncbi_nrp_id": "WP_010869361.1", "uniref100_id": "UniRef100_P47415", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133C15", "RefSeq:WP_010869361.1", "UniRef:UniRef100_P47415" ] }, "ips": { "uniref100_id": "UniRef100_P47415", "uniref90_id": "UniRef90_P47415" }, "psc": { "uniref90_id": "UniRef90_P47415", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_P47415", "cog_id": "COG0200", "cog_category": "J", "kegg_orthology_id": "K02876", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47415" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "KKNAAMEMLJ_388", "locus": "GCA000027325_00352" }, { "type": "cds", "sequence": "contig_1", "start": 199610, "stop": 201037, "strand": "+", "frame": 2, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0005048", "GO:0005886", "GO:0006616", "GO:0008320", "GO:0015031", "GO:0016020", "GO:0031522", "GO:0043952", "RefSeq:WP_009885855.1", "SO:0001217", "UniParc:UPI00001357E5", "UniRef:UniRef100_P47416", "UniRef:UniRef50_O52351", "UniRef:UniRef90_P47416" ], "nt": "ATGCAAACTGTTTCTTCACCCAAACAAAAACTTAACTTTGGTCAAAGGTTACTAACTCTATTACAGAACCGTGACTTTATGGTGTCGCTGGTTTTAACAGTGGTACTTTTAATCTTGTTTAGGGTGTTAGCAATTATCCCCTTACCAGGGATTAGGATTAATGAGAGTGTCTTGGATAGAAATTCCAATGACTTTTTTTCACTTTTTAACTTACTTGGGGGTGGGGGATTAAACCAGCTATCGTTGTTTGCAGTTGGGATCAGTCCTTATATCTCAGCCCAAATCATCATGCAACTGCTTTCAACTGATCTAATTCCTCCACTTTCAAAGCTAGTTAACAGTGGGGAAGTGGGGCGAAGAAAGATTGAGATGATCACAAGAATTATCACCTTACCCTTTGCTTTAGTGCAAGCATTTGCTGTGATCCAAATTGCTACTAATGCAGGCACTGGTTCAAGTCCGATTAGTTTAGCTAATAGTGGCAGTGAGTTTATTGCTTTTTATATTATTGCTATGACTGCAGGGACTTATATGGCAGTGTTTTTGGGTGATACTATCTCCAAAAAAGGGGTTGGTAATGGGATTACTTTGTTAATTCTCTCAGGGATTTTATCCCAACTCCCCCAGGGCTTTATTGCTGCTTACAATGTTTTGAGTGGGATAGTAATTACTCTAACCCCACAGTTAACTGCAGCAATTAGCTTCTTTATCTATTTCTTAGCATTCTTAGTTTTACTGTTTGCCACTACCTTTATCACCCAAGCGACCAGAAAGATTCCCATCCAACAATCAGGACAAGGGTTGGTTAGTGAAGTCAAAACCTTACCTTATTTGCCTATTAAGGTGAATGCTGCTGGGGTGATCCCTGTCATCTTTGCATCCAGTATTATGTCTATCCCTGTGACCATTGCCCAGTTTCAACCCCAAACTGAGTCACGGTGGTTTGTGGAGGATTACCTATCACTTTCAACACCCGTAGGGATCTTTTTATATGCAGTTTTGGTTATCCTTTTTTCCTTTTTTTACAGTTACATCCAGATTAACCCAGAACGGTTAGCTAAGAACTTTGAAAAATCTGGCAGATTTATCCCAGGGATTCGACCGGGCAATGATACAGAGAAACACATTGCGCGGGTGTTAATAAGGATTAACTTTATAGGTGCTCCTTTTTTAACTGTTATTGCTATTATCCCTTACATTGTTTCTTATTTCATTAGGTTACCTAACTCCTTGAGTTTAGGGGGGACGGGGATTATTATTATTGTTACTGCTGTAGTTGAATTTATCAGTGCACTGCGTTCAGCTGCTACTGCTACTAACTACCAACAACTAAGGAGAAACTTAGCAATTGAAGTGCAACAAACAGCTAAACAAGATAGTCTAGAGCAGCTTCAAAAAGAAGCACCAGGGATTGGTAACCTATGGTAG", "aa": "MQTVSSPKQKLNFGQRLLTLLQNRDFMVSLVLTVVLLILFRVLAIIPLPGIRINESVLDRNSNDFFSLFNLLGGGGLNQLSLFAVGISPYISAQIIMQLLSTDLIPPLSKLVNSGEVGRRKIEMITRIITLPFALVQAFAVIQIATNAGTGSSPISLANSGSEFIAFYIIAMTAGTYMAVFLGDTISKKGVGNGITLLILSGILSQLPQGFIAAYNVLSGIVITLTPQLTAAISFFIYFLAFLVLLFATTFITQATRKIPIQQSGQGLVSEVKTLPYLPIKVNAAGVIPVIFASSIMSIPVTIAQFQPQTESRWFVEDYLSLSTPVGIFLYAVLVILFSFFYSYIQINPERLAKNFEKSGRFIPGIRPGNDTEKHIARVLIRINFIGAPFLTVIAIIPYIVSYFIRLPNSLSLGGTGIIIIVTAVVEFISALRSAATATNYQQLRRNLAIEVQQTAKQDSLEQLQKEAPGIGNLW", "aa_hexdigest": "6e776e11b4f501d8e4e80c02c2838412", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001357E5", "ncbi_nrp_id": "WP_009885855.1", "uniref100_id": "UniRef100_P47416", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001357E5", "RefSeq:WP_009885855.1", "UniRef:UniRef100_P47416" ] }, "ips": { "uniref100_id": "UniRef100_P47416", "uniref90_id": "UniRef90_P47416" }, "psc": { "uniref90_id": "UniRef90_P47416", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_O52351", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0005048", "GO:0005886", "GO:0006616", "GO:0008320", "GO:0015031", "GO:0016020", "GO:0031522", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_O52351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52351" ], "product": "Protein translocase subunit SecY" }, "genes": [ "secY" ], "id": "KKNAAMEMLJ_389", "locus": "GCA000027325_00353" }, { "type": "cds", "sequence": "contig_1", "start": 201031, "stop": 201675, "strand": "+", "frame": 1, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "RefSeq:WP_009885856.1", "SO:0001217", "UniParc:UPI000012DBCE", "UniRef:UniRef100_P47417", "UniRef:UniRef50_P47417", "UniRef:UniRef90_P47417" ], "nt": "ATGGTAGCACAGTTTAATAAGTTCATTATCTTAGGACCCCCAGGGGCAGGAAAAGGTACAGTTTGTAAACTGCTTAGCAAAACAACTAAGTTAGTCCATATTGCTAGTGGTGATCTGTTTAGAGAAGCCATTAAAAACCAGAGTGTTATTGGTAGAAAGATTGCAGCAATTATCAGTCAGGGTGGTTATGTTGATGATGCCACTACTAACCAGCTTGTTTATGAATATATCACTACCAATCCATTACCAAATGGTTTTATCTTAGATGGTTATCCAAGAACAGAGAACCAGCTTGATTTTCTAAATATTAAACTAACCATTGACATGGTCTTTGAACTAGTTGTTAGTGATCTGAATAAACTGATTACACGGATTGATAACAGGGTTATTTGTAACAACTGTAACAGTGTTTATAACTTGCTTTTTCAAAAACCACTAGTTGAAAATAGTTGTGATCAGTGTTCAGCTAAACTAGTGAAAAGGAGTGATGATAACAAAGCAGTGGTCAAAGCAAGAATGGAGTTATATCAACAAACAATTCAACCAATCCACACTTACTTTTTCAACAAACAACTTTTAGTACAAATTGATTGCTTTTTACCACTAGAAGAACAACTCAAGACAATCAAACAATTTATTAGATAA", "aa": "MVAQFNKFIILGPPGAGKGTVCKLLSKTTKLVHIASGDLFREAIKNQSVIGRKIAAIISQGGYVDDATTNQLVYEYITTNPLPNGFILDGYPRTENQLDFLNIKLTIDMVFELVVSDLNKLITRIDNRVICNNCNSVYNLLFQKPLVENSCDQCSAKLVKRSDDNKAVVKARMELYQQTIQPIHTYFFNKQLLVQIDCFLPLEEQLKTIKQFIR", "aa_hexdigest": "2d2e4a1cf1592a93efcbc610a0b04d94", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012DBCE", "ncbi_nrp_id": "WP_009885856.1", "uniref100_id": "UniRef100_P47417", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DBCE", "RefSeq:WP_009885856.1", "UniRef:UniRef100_P47417" ] }, "ips": { "uniref100_id": "UniRef100_P47417", "uniref90_id": "UniRef90_P47417" }, "psc": { "uniref90_id": "UniRef90_P47417", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_P47417", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_P47417", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47417" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "KKNAAMEMLJ_390", "locus": "GCA000027325_00354" }, { "type": "cds", "sequence": "contig_1", "start": 201678, "stop": 202424, "strand": "+", "frame": 3, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006", "KEGG:K01265", "RefSeq:WP_009885857.1", "SO:0001217", "UniParc:UPI0000125A04", "UniRef:UniRef100_P47418", "UniRef:UniRef50_P47418", "UniRef:UniRef90_P47418" ], "nt": "ATGATCTATCTCAAATCTGCAAATGAAGTTGCAGGGATTAAAAAAGCATGTGCAATCTTCAAAGCAGTTAAGGCATATTTTACAATTGAAAAGTTACTTGGCAAAAAGTTGGTTACCATTGATCGTTTAATCAAACAATTCATTGAACAAAAACAAGCTAAATGTGCGTTTCATGGTTATCTAGGTTTCCCTGGTTTTAACTGTCTATCGTTAAACCAAACGGTTATCCATGGAGTTGCCGATCAAACTGTTTTTAAAGATAGTGATAAACTAACGCTTGACATTGGGATAGACTATCATGGTTATCTTTGTGATGCAGCTTTCACTTTACTTGGTAATAAAGCTGATCCAAAGGCAGTAAAACTGTTAAATGATGTTGAACAAGCATTTAGTAAGGTAATTGAACCTGAGCTATTTGTTAACAATCCGATTGGTAATTTATCCAATGCGATCCAAACTTACTTTGAAAACAAGGGCTATTTTCTTGTCAAAGAGTTTGGGGGTCATGGTTGTGGGATTAAGATCCATGAAGATCCTTTAATCTTAAACTGGGGAGAGAAAAACCAGGGCGTTAGGTTACAAGAGGGGATGGTAATCTGTATTGAACCGATGGTTATGACTGATAGTAGTGAGATAACAATGGCAGCTAACAACTGGAATGTACTAACTTTAAAGAGTAAGTTTAACTGTCATGTGGAACAGATGTATCACATCACAAACAACGGCTTTGAATGTTTAACTAACTAA", "aa": "MIYLKSANEVAGIKKACAIFKAVKAYFTIEKLLGKKLVTIDRLIKQFIEQKQAKCAFHGYLGFPGFNCLSLNQTVIHGVADQTVFKDSDKLTLDIGIDYHGYLCDAAFTLLGNKADPKAVKLLNDVEQAFSKVIEPELFVNNPIGNLSNAIQTYFENKGYFLVKEFGGHGCGIKIHEDPLILNWGEKNQGVRLQEGMVICIEPMVMTDSSEITMAANNWNVLTLKSKFNCHVEQMYHITNNGFECLTN", "aa_hexdigest": "776ed85c8b07d493b4167d8c962b87b0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125A04", "ncbi_nrp_id": "WP_009885857.1", "uniref100_id": "UniRef100_P47418", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125A04", "RefSeq:WP_009885857.1", "UniRef:UniRef100_P47418" ] }, "ips": { "uniref100_id": "UniRef100_P47418", "uniref90_id": "UniRef90_P47418" }, "psc": { "uniref90_id": "UniRef90_P47418", "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_P47418", "cog_id": "COG0024", "cog_category": "J", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_P47418", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47418" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "KKNAAMEMLJ_391", "locus": "GCA000027325_00355" }, { "type": "cds", "sequence": "contig_1", "start": 202424, "stop": 202636, "strand": "+", "frame": 2, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022", "KEGG:K02518", "RefSeq:WP_009885858.1", "SO:0001217", "UniParc:UPI000012D262", "UniRef:UniRef100_P47419", "UniRef:UniRef50_Q50298", "UniRef:UniRef90_P47419" ], "nt": "ATGAAAAACGATAAACTCTTTCTAACAGGTAAGATACTGGAAATTATCCATGGTGATAAGTACCGGGTGATGCTTGAAAACAATGTTGAGGTTGATGCACATCTAGCAGGTAAAATGAAGATGAAAAGAACCAAGATTCTCCCTGGGGATGTTGTTGAGGTGGAATTTTCTCCCTATGATTTGAAACTAGGTAGGATAACCCAAAGAAAATAA", "aa": "MKNDKLFLTGKILEIIHGDKYRVMLENNVEVDAHLAGKMKMKRTKILPGDVVEVEFSPYDLKLGRITQRK", "aa_hexdigest": "cb34f5de7123f10488fac58fbf88077f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012D262", "ncbi_nrp_id": "WP_009885858.1", "uniref100_id": "UniRef100_P47419", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D262", "RefSeq:WP_009885858.1", "UniRef:UniRef100_P47419" ] }, "ips": { "uniref100_id": "UniRef100_P47419", "uniref90_id": "UniRef90_P47419" }, "psc": { "uniref90_id": "UniRef90_P47419", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q50298", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q50298", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50298" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "KKNAAMEMLJ_392", "locus": "GCA000027325_00356" }, { "type": "cds", "sequence": "contig_1", "start": 202760, "stop": 203017, "strand": "+", "frame": 2, "gene": null, "product": "30S ribosomal protein S13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "nt": "ATGGCACGAATCTTAGGGATTGATATCCCCAACCAAAAACGGATCGAGATAGCTTTAACATACATCTTTGGGATTGGTTTGTCAAGTGCAAAAACAATCTTAAAAAAAGCAAAGATTAACCCTGATAAACGCGTTAAAGATCTGAGTGAAGAGGAACTTGTTGCGATTAGAAACGCAGCAAGCGGTTACAAGATTGAGGGTGATTTGAGAAGAGAGATTGCTTTAAACATCAAACACCTAACAGAGATCGGTTCTTGA", "aa": "MARILGIDIPNQKRIEIALTYIFGIGLSSAKTILKKAKINPDKRVKDLSEEELVAIRNAASGYKIEGDLRREIALNIKHLTEIGS", "aa_hexdigest": "11c6e5322ee6e5b4fc1e95a948b562ec", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031B5D25D", "query_cov": 1.011764705882353, "subject_cov": 0.9885057471264368, "identity": 0.57, "score": 95.5, "evalue": 4.27e-24, "valid": false, "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_A0A535XWH1", "kegg_orthology_id": "K02952" }, "pscc": { "uniref50_id": "UniRef50_A0A535XWH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "product": "30S ribosomal protein S13" }, "genes": [], "id": "KKNAAMEMLJ_393", "locus": "GCA000027325_00357" }, { "type": "cds", "sequence": "contig_1", "start": 203320, "stop": 203499, "strand": "+", "frame": 1, "gene": "rpsK", "product": "30S ribosomal protein S11", "db_xrefs": [ "COG:COG0100", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "KEGG:K02948", "SO:0001217", "UniRef:UniRef50_P47422", "UniRef:UniRef90_P47422" ], "nt": "GTGGCTAAAACTGTGAAAGAGATGGGAATGGGGAGTGTTAAGATGTATCTGAAGGGAACAGGTAGAGGAAAAGACACCACGATTAGAAGCTTTGCTAATGCTGGGATTACGATCACAGAAATCAATGAAAAAACCCCTATTCCCCACAATGGCTGCAAGCCTCCTAAGCGTCCGCGCTAA", "aa": "MAKTVKEMGMGSVKMYLKGTGRGKDTTIRSFANAGITITEINEKTPIPHNGCKPPKRPR", "aa_hexdigest": "1ffbae663cd83fb40a4b2d12bf80cca3", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_K2BZH6", "query_cov": 0.9322033898305084, "subject_cov": 0.8461538461538461, "identity": 0.509, "score": 67.4, "evalue": 1.11e-13, "valid": false, "product": "Small ribosomal subunit protein uS11", "uniref50_id": "UniRef50_K2BZH6" }, "genes": [ "rpsK" ], "truncated": "5-prime", "pseudogene": { "start": 203134, "stop": 203499, "observations": { "mutation": [ 203242 ], "stop": [ 203242 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47422", "query_cov": 1.0, "subject_cov": 0.48760330578512395, "identity": 0.983, "score": 123.0, "evalue": 2.97e-35, "gene_start": 1, "gene_end": 59, "reference_start": 63, "reference_end": 121, "reference_sequence": "MAKKKKINVPSGLIHVSCSPNNTIVSATDPSGNVLCWASSGTVGFKGFRKKTPYSAGVAADKVAKTVKEMGMGSVKMYLKGTGRGKDTTIRSFANAGITITEINEKTPIPHNGCKPPKRPR" }, "paralog": false, "identity": 0.9917355371900827, "score": 244.0, "evalue": 1.94e-85, "description": "Internal stop codon at 203242. Nonsense mutation around 203242.", "psc": { "uniref90_id": "UniRef90_P47422", "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_P47422", "cog_id": "COG0100", "cog_category": "J", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47422", "UniRef:UniRef50_P47422", "COG:COG0100", "COG:J", "KEGG:K02948", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47422" ], "product": "Small ribosomal subunit protein uS11" } }, "id": "KKNAAMEMLJ_394", "locus": "GCA000027325_00358" }, { "type": "cds", "sequence": "contig_1", "start": 203515, "stop": 203844, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01193.29" ], "nt": "ATGGAAAAATTTTTAAAGTACGAAATTAAGGTTAACAACAACCAACCAACCAACACTAACCCTAACTATGGGATCTTTGAAGTAGCACCGTTAGAATCAGGATTTGGGATTACCATTGGTAATGCGATGCGCCGAGTGTTACTTAGTTGTATCCCAGGCGCTAGTGTGTTTGCCATTGCCATTAGTGGGGTAAAACAAGAGTTTAGTAATGTGGAGGGTGTGTTGGAAGATGTGACTGAAATGGTGTTAAACTTCAAGCAACTAGTGGTGAGAATCTCTGATCTTTTGTTTGAAGATGGGGAGATGATCGAACCACCCTTAGAAAGGTGA", "aa": "MEKFLKYEIKVNNNQPTNTNPNYGIFEVAPLESGFGITIGNAMRRVLLSCIPGASVFAIAISGVKQEFSNVEGVLEDVTEMVLNFKQLVVRISDLLFEDGEMIEPPLER", "aa_hexdigest": "b0289ee9d10a5f7ece465eca25a24da6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01193.29", "name": "RNA_pol_L", "length": 43, "aa_cov": 0.3944954128440367, "hmm_cov": 0.6976744186046512, "evalue": 2.260111547699439e-10, "score": 35.22905731201172, "start": 30, "stop": 72 } ], "seq_stats": { "molecular_weight": 12135.856599999996, "isoelectric_point": 4.564591789245606 }, "id": "KKNAAMEMLJ_395", "locus": "GCA000027325_00359" }, { "type": "cds", "sequence": "contig_1", "start": 203920, "stop": 204501, "strand": "+", "frame": 1, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P47423", "UniRef:UniRef90_P47423" ], "nt": "GTGATTAATAAGGACCTTTATCTCTTCTCTTTACAAAAGGACATGAAACTAACAGTCAGTGTTTATGTTAAACAGGGTAGGGGCTTTACTAGCTTTCTTGAAAACAGAGAATTGATCAATTCGCTTGGCATTATTGCTACAGATGCTAACTTTTCCCCGGTTTTACACTGTGGTTATGAAGTTCAAGAGGTGAAAACTTCCAAACAAAAGTTAACTGACCATCTCACCTTTAAGATTGCTACTAACGGTGCAATTAAAGCAGTGGATGCGTTTGCTATGGCAGCAAAGATCCTAATTGAACACTTAAACCCAATTGTAAGTGTCAATGAGTCAATTAAGAATTTAACAATTATCCAAGAGAAAGCAGAGGAAAGAAAGGTGAAATCATTTGCCAAGCAAATTGAAGAACTTGACTTTACTGTTAGAACCTTTAACTGTTTGAAAAGAAGTGGGATCCACACACTCCAAGAGTTACTATCAAAGTCATTAACTGACATTAGAGAGATTAGAAACCTAGGTAAGAAATCAGAACGGGAGATTATCAAAAAGGTGCAAGAGTTAGGTTTAAAATTCCGTTCTTAA", "aa": "MINKDLYLFSLQKDMKLTVSVYVKQGRGFTSFLENRELINSLGIIATDANFSPVLHCGYEVQEVKTSKQKLTDHLTFKIATNGAIKAVDAFAMAAKILIEHLNPIVSVNESIKNLTIIQEKAEERKVKSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLTDIREIRNLGKKSEREIIKKVQELGLKFRS", "aa_hexdigest": "8bed4181bdd3df351534ad8cf2774526", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpoA" ], "truncated": "5-prime", "pseudogene": { "start": 203620, "stop": 204501, "observations": { "mutation": [ 203842 ], "stop": [ 203842 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47423", "query_cov": 0.9948186528497409, "subject_cov": 0.5853658536585366, "identity": 1.0, "score": 362.0, "evalue": 4.08e-124, "gene_start": 2, "gene_end": 193, "reference_start": 137, "reference_end": 328, "reference_sequence": "MEKFLKYEIKVNNNQPTNTNPNYGIFEVAPLESGFGITIGNAMRRVLLSCIPGASVFAIAISGVKQEFSNVEGVLEDVTEMVLNFKQLVVRISDLLFEDGEMIEPPLERWPVLKVTAEKKGAVYAKDLECPAGFEVINKDLYLFSLQKDMKLTVSVYVKQGRGFTSFLENRELINSLGIIATDANFSPVLHCGYEVQEVKTSKQKLTDHLTFKIATNGAIKAVDAFAMAAKILIEHLNPIVSVNESIKNLTIIQEKAEERKVKSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLTDIREIRNLGKKSEREIIKKVQELGLKFRS" }, "paralog": false, "identity": 0.9965870307167235, "score": 559.0, "evalue": 1.66e-204, "description": "Internal stop codon at 203842. Nonsense mutation around 203842.", "psc": { "uniref90_id": "UniRef90_P47423", "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47423", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47423", "EC:2.7.7.6", "UniRef:UniRef50_P47423", "COG:COG0202", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P47423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47423" ], "product": "DNA-directed RNA polymerase subunit alpha" } }, "id": "KKNAAMEMLJ_396", "locus": "GCA000027325_00360" }, { "type": "cds", "sequence": "contig_1", "start": 204559, "stop": 204885, "strand": "+", "frame": 1, "gene": "rplQ", "product": "50S ribosomal protein L17", "db_xrefs": [ "COG:COG0203", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02879", "SO:0001217", "UniRef:UniRef50_P47424", "UniRef:UniRef90_P47424" ], "nt": "ATGACAGTGCGTCAGCAAGTGAGTGCAGTGTTAAGTTATGGAAAGATTCAAACCACTTTAAAAAAAGCTAAGAACACCCAAAAAAGGTTAGAGAAGATTATTACCATTGCTAAAGTTGATAACTTTAACAACCGCAGGGCTGTTAAAAAGTGGTTATTAAATACCAATTCATTAGATGTAGATCAACTCACAAACCACCTTTTTAAAAAAGTAGCACCACGTTTTTTAAAGCGTAATGGTGGTTATAGTAGAGTGTTAAAGTTGGGAGTTAGAAGGGGTGATAGTACTGAAATGGCGATCTTACAGCTGATAGATGCTACCAACTAA", "aa": "MTVRQQVSAVLSYGKIQTTLKKAKNTQKRLEKIITIAKVDNFNNRRAVKKWLLNTNSLDVDQLTNHLFKKVAPRFLKRNGGYSRVLKLGVRRGDSTEMAILQLIDATN", "aa_hexdigest": "7a02b700a388aeaa2722ac9241ee35b4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47424", "query_cov": 1.0, "subject_cov": 0.8780487804878049, "identity": 1.0, "score": 208.0, "evalue": 7.13e-68, "valid": true, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P47424", "cog_id": "COG0203", "cog_category": "J", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47424" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [ "rplQ" ], "id": "KKNAAMEMLJ_397", "locus": "GCA000027325_00361" }, { "type": "cds", "sequence": "contig_1", "start": 204872, "stop": 205684, "strand": "+", "frame": 2, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:7.-.-.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47425", "UniRef:UniRef90_P47425" ], "nt": "ATGCTACCAACTAAACAAGCTGCTTGTAGTTTTATTAATGTTGCTTTTTCCTATAATGAACTGCCATTAATTAGGGAACTATCTTTTAGTGTTTATGAGGGGGAATATGTTTGTATTGTTGGTCATAATGGCAGTGGTAAATCAACCATTTCCAAACTGTTAACAGGGTTATTAAAGCCCCAGGCAGGTGAGATTAAGATCTTTGGTAAAACAGTTGATTTTGATAATGTTAGTTACTTGAGAAATAACATTGGGATCATCTTTCAAAACCCTGATAACCAGTTTATTGGGATCACTGTTGAAGATGACATTGCCTTTGGGCTTGAAAACAAGTGTTTTTCAAGACAGAAGATAAAAGCCATTATTGATGAAGTTACCCTACAAACCCAAACTGATGGGTTTATTAAACAAGAACCCCATAACCTATCAGGGGGACAAAAACAACGGGTAGCAATTGCATCTGTTTTAGCACTAAATCCTGCTATTATCATCTTTGATGAATCAACTGCGATGTTAGATCCTAAAGCTAAAAAAACGATTAAGCAGTTTATGGTTAAACTAGCCAAACAGGGCAAGTGTGTGATCTCAATTACCCATGATATGGAAGAAGTTACTAAAGCTGATAAGGTGTTAGTAATGAATGAGGGCAAACTGATCAAACAAGGTAAACCTGTTGAAGTTTTCACTAGTGAACAAGAGTTACAAAAAATCCGTTTAGACATCCCTTTTTCACTCAGTCTTTCAACCAAGATAAGAGGGATCACTAGTACAATTGATTACCAAACCCTGATTAAATCAATTGCCAAGCTGTGA", "aa": "MLPTKQAACSFINVAFSYNELPLIRELSFSVYEGEYVCIVGHNGSGKSTISKLLTGLLKPQAGEIKIFGKTVDFDNVSYLRNNIGIIFQNPDNQFIGITVEDDIAFGLENKCFSRQKIKAIIDEVTLQTQTDGFIKQEPHNLSGGQKQRVAIASVLALNPAIIIFDESTAMLDPKAKKTIKQFMVKLAKQGKCVISITHDMEEVTKADKVLVMNEGKLIKQGKPVEVFTSEQELQKIRLDIPFSLSLSTKIRGITSTIDYQTLIKSIAKL", "aa_hexdigest": "fd1daccdb08b4cf1eb49fb1f1e4864a0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47425", "query_cov": 1.0, "subject_cov": 0.9854014598540146, "identity": 1.0, "score": 521.0, "evalue": 1.69e-186, "valid": true, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "7.-.-.-" ], "uniref50_id": "UniRef50_P47425", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_P47425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47425" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA1" ], "id": "KKNAAMEMLJ_398", "locus": "GCA000027325_00362" }, { "type": "cds", "sequence": "contig_1", "start": 205681, "stop": 206595, "strand": "+", "frame": 1, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:3.6.3.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_009885865.1", "SO:0001217", "UniParc:UPI00001393F5", "UniRef:UniRef100_P47426", "UniRef:UniRef50_Q50293", "UniRef:UniRef90_P47426" ], "nt": "GTGAAAAAAAAGATAGTCCCAATTAACCCTTTAAAAGCAGATGAGATTTTAGCAGTTAGTCACTTATCATGTGTTTTTAACAGTAAAACTAACAATCCCATTAAGGTGATTGATGATTTTTCCTATACCTTTCAAAAGAACCAAATTTACTGTATTATTGGTGATAGTGGCAGTGGTAAATCAACCCTTGTTAACCACTTCAATGGGTTGATAAAACCCAACCAAGGTGATATTTGGGTTAAAGATATCTATATTGGTGCTAAACAACGCAAGATTAAGAACTTTAAAAAACTGCGAAAAACTATCTCAATTGTTTTCCAGTTTCCTGAGTACCAATTGTTTAAAGATACCGTGGAAAAAGACATTATGTTTGGTCCAGTAGCATTAGGTCAATCCAAGTATGATGCGCGCCAAAAAGCGGCTTATTATCTGGAGATGATGGGGTTAAAATACCCTTTTTTAGAACGTAATCCCTTTGAATTGAGTGGGGGGCAGAAAAGAAGGGTAGCGATTGCTGGTATACTTGCAATTGAACCAGAAATTCTAATCTTTGATGAACCAACTGCTGGGCTTGATCCTGAAGGGGAAAGGGAGATGATGCAGTTAATTAAAACTGCCAAACAACAACAAAGAACGGTATTTATGATCACCCACCAGATGGAAAATGTCCTTGAGGTGGCTGATGTGGTTTTGGTTTTAGCTAAGGGTAAACTAGTAAAAGCTGCTAGTCCATATGAAGTGTTTATGGACCAAACTTTCCTTGAAAAAACAACGATTGTTCTCCCCCCTGTGATCCAAGTGATCAAAGATCTAATTGCGATTAATGCTCACTTTAATAAGTTAATTGAGTTGCAACCAAAGAACCTAGAACAGCTTGCATCAGCAATTAACAAGACTATAGCAAACCATGGATAA", "aa": "MKKKIVPINPLKADEILAVSHLSCVFNSKTNNPIKVIDDFSYTFQKNQIYCIIGDSGSGKSTLVNHFNGLIKPNQGDIWVKDIYIGAKQRKIKNFKKLRKTISIVFQFPEYQLFKDTVEKDIMFGPVALGQSKYDARQKAAYYLEMMGLKYPFLERNPFELSGGQKRRVAIAGILAIEPEILIFDEPTAGLDPEGEREMMQLIKTAKQQQRTVFMITHQMENVLEVADVVLVLAKGKLVKAASPYEVFMDQTFLEKTTIVLPPVIQVIKDLIAINAHFNKLIELQPKNLEQLASAINKTIANHG", "aa_hexdigest": "4db8cad8c5695dfd3904d297caf5ae55", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001393F5", "ncbi_nrp_id": "WP_009885865.1", "uniref100_id": "UniRef100_P47426", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393F5", "RefSeq:WP_009885865.1", "UniRef:UniRef100_P47426" ] }, "ips": { "uniref100_id": "UniRef100_P47426", "uniref90_id": "UniRef90_P47426" }, "psc": { "uniref90_id": "UniRef90_P47426", "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "3.6.3.-" ], "uniref50_id": "UniRef50_Q50293", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_Q50293", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50293" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" }, "genes": [ "ecfA2" ], "id": "KKNAAMEMLJ_399", "locus": "GCA000027325_00363" }, { "type": "cds", "sequence": "contig_1", "start": 206588, "stop": 206923, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAACTTTATTAATGGCTATATCCCAAGAAACAGCTTTGTTCACAAGCTGCATCCAACTACTAAACTAGTAATCTTTTTACTGTTAGTTATCTTGGTATTTGTACCAATTGGCTTTGTTTTTCAAAGTGTTATCTTTCTTTTTGTTACCTTTGTTTTTTTTATTGCTAAACTCCCGGGGCGGTTTTACAGTTCAGCAATTAAGTCAATTACGCTGTTATTTCTCTTGTTGTTATTTGTAAACTGGTTTACCTTTCGTGATCCAGGGTTTTATCTTACTAGTGATCAACTTAACAGTTTACCAGCCATTGATAACAGCAAGTTTAGCTTTTGA", "aa": "MDNFINGYIPRNSFVHKLHPTTKLVIFLLLVILVFVPIGFVFQSVIFLFVTFVFFIAKLPGRFYSSAIKSITLLFLLLLFVNWFTFRDPGFYLTSDQLNSLPAIDNSKFSF", "aa_hexdigest": "afd990e90f8e6db4d241d4ea86bf3ffc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12867.17819999998, "isoelectric_point": 9.774519157409667 }, "id": "KKNAAMEMLJ_400", "locus": "GCA000027325_00364" }, { "type": "cds", "sequence": "contig_1", "start": 206933, "stop": 207394, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAACTATCAAGATAATGTTTTTTCCCAGGTTTTTGCTTTTAACAGGGGTAATTTAACCAACTTAAATCAACTTGATTTTTTCTATAAAGCTAACAATGCTGATAGTTACACCAAAGTAAAGGGCATTGATAGTTTAGCAAGTATGCTAGCAAACAATGGCAATGGTTTAAGCAAAGACAAAATTCTGAGTGCTTTTTTAGATCACAATTTAAACCTTTATTTAGCGAGAAGTTGGGGGGCAAATTTTGCTGGGTTTGTTGTTGATTTTAACCCAACAACCCAACTCTTTAAACTCACCCCTTTTCTAGCAAATGCTAGTTATGTTTTAACGTTAAGAGCAGTTATCTTAGCATTCTATGTCACCCAAAAGATCCTAATTATGATCTTATTTGCAACTGTACTCACTTCCACTTCAAGTTCAGTTGAACTAGCATATGGGATTGAAAGGTTATTATGA", "aa": "MFNYQDNVFSQVFAFNRGNLTNLNQLDFFYKANNADSYTKVKGIDSLASMLANNGNGLSKDKILSAFLDHNLNLYLARSWGANFAGFVVDFNPTTQLFKLTPFLANASYVLTLRAVILAFYVTQKILIMILFATVLTSTSSSVELAYGIERLL", "aa_hexdigest": "331d99e514f5958aa36d2962e941fdec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17091.436999999994, "isoelectric_point": 8.891173362731934 }, "id": "KKNAAMEMLJ_401", "locus": "GCA000027325_00365" }, { "type": "cds", "sequence": "contig_1", "start": 207434, "stop": 207850, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCATTGCCATTGCCATTAGGTTTGTCCCTTCCTTGTTACTAGAATCACAACGGATCTTAAATGCCCAAGCCTCCAGGGGTTTAGACTTTCGAAATGGGGGATTTTTAGTGAAGATGCGTTCACTCTCTTCGTTAGTAGTGCCAATGGTTTCCATTGCCTTTCGCAATGCCTCTGAACTTGCTAGTGCAATGGAAGCAAGGGGTTATCACCCTGCAAAGAAGCGCAGTAGTTATAGACAATACAAAATCACTTGGATTGATATTTTAGCGTTATTTTTGGTTTTTGCTTGGTTTGTTGTGATTATCTTTTTAACTATTAGAGGAGCGGTCTTTTTGGATCTAGGTACACCAGAATGGTTATTAACAGGAAAGATTAATGAACAGGTAATCAGGGATCTGAAGGTAAGTGGCTAG", "aa": "MTIAIAIRFVPSLLLESQRILNAQASRGLDFRNGGFLVKMRSLSSLVVPMVSIAFRNASELASAMEARGYHPAKKRSSYRQYKITWIDILALFLVFAWFVVIIFLTIRGAVFLDLGTPEWLLTGKINEQVIRDLKVSG", "aa_hexdigest": "53a7f7fc7624b89475d6c42af38c4bae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15532.232999999984, "isoelectric_point": 10.462010002136228 }, "id": "KKNAAMEMLJ_402", "locus": "GCA000027325_00366" }, { "type": "cds", "sequence": "contig_1", "start": 207933, "stop": 208577, "strand": "+", "frame": 3, "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "db_xrefs": [ "COG:COG0101", "COG:J", "EC:5.4.99.12", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147", "SO:0001217", "UniRef:UniRef50_P47428", "UniRef:UniRef90_P47428" ], "nt": "TTGGAGCAAAGTTTTTCATTAATCATTGGCAGAAAGATAAAGGTAATTGGTTCAGGTAGAACTGATAAAGGGGTACATGCCATCAACCAAACCTTTCATGTTGATATTAATGGTGAAATTAATCTCAATTTGTTAATTAGAAAAATTAACCAGTTGATTAAGCCCCACTGTATAGTTAAAACCTTGGTATTGGTTAACGATAGCTTTCATGCGCGGTTTCAAGTTAAAACCAAGGTGTATGAATATCTGATTAACTGTGGGAATTTAAATCCGTTGCAATTTAACTATGTTTGGCAGTTAAACCAGCAATTGGATCTTGAAAAACTCAAAGCTGATGCCACTTTATTTTTAGGTAAGAAAAACTTTCTTAGCTTCAGTAGTTCGATTCACACTGATTCAATTCGCACAATTAGTAAAATTACCATACAAAAAGAAACTAACCAACTAGTTAGACTAACTTTTTTTGGCAGTGGTTTTCTCAGGAGTCAAGTGAGGATGATAGTTGCTTGTTTAGTGAATTTAAACACTAATAAAATGGCACTTGAAACAGTTGCAAAATTGTTTGAACACCCCAAGAAAGGGAGTTGTGTTGTTAAAGCCCCTAGTTGTGGTTTGTATCTGAAAACAGTGGTATATGAAAAATAG", "aa": "MEQSFSLIIGRKIKVIGSGRTDKGVHAINQTFHVDINGEINLNLLIRKINQLIKPHCIVKTLVLVNDSFHARFQVKTKVYEYLINCGNLNPLQFNYVWQLNQQLDLEKLKADATLFLGKKNFLSFSSSIHTDSIRTISKITIQKETNQLVRLTFFGSGFLRSQVRMIVACLVNLNTNKMALETVAKLFEHPKKGSCVVKAPSCGLYLKTVVYEK", "aa_hexdigest": "746a0dd3964a5b1fb4c3cce16223b794", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47428", "query_cov": 1.0, "subject_cov": 0.8770491803278688, "identity": 0.995, "score": 422.0, "evalue": 6.17e-149, "valid": true, "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "ec_ids": [ "5.4.99.12" ], "uniref50_id": "UniRef50_P47428", "cog_id": "COG0101", "cog_category": "J", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147" ] }, "pscc": { "uniref50_id": "UniRef50_P47428", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47428" ], "product": "tRNA pseudouridine synthase A" }, "genes": [ "truA" ], "id": "KKNAAMEMLJ_403", "locus": "GCA000027325_00367" }, { "type": "cds", "sequence": "contig_1", "start": 208567, "stop": 209211, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGTTACAAGTGGGATCTATCAGTTTTATTAAACAACCAAAGCTTACAAGCAAATTTTTTAAAAATTCAAACAGTTAGTGAAGCGTTAATTAAAGCTTATAACAACGGGTTGTGTTTTACAAATAAAACTAGCTTTGAACAGTTTTTAGCAATCGATGATAAGTTCACTGAACTTGAAAATCGTTACACTAACTACCTTTATAACAAGCAGAATGAAAATAACTTGGATAAGGAGGTTAATGATGCAATTTTTGCATACCAGAGTTTTAAAAATAACCATAACCTTGCTTTCAGTACACTGCAACAGGAGTTATATAACCATGAAAAACTCATTAAGGATTATTTAACTGATCCAAAGCTAGCGGTTTACAAGCGCAACTTAATGTTAGTTTTTCGCGATAAACCCCACCAACTATCTAGTCAAACCCAGAGTTTATTGAGTCAAATTAACCCTTGTTTTAACCAAGCAGAACGGATTTTTAACATCCTTTCAACTGCTGATTTAAACTTGCAACCTGTTGTTTATCAAAACAAAAAATATCCGATTAACAGTGTGAGTGATTATCAGTCCTTACTTGAAAACACTAACAGGGGGATTAGAAAAGCTTGTTATGAGAAGTGGATTGAAATTTATTGA", "aa": "MKNSYKWDLSVLLNNQSLQANFLKIQTVSEALIKAYNNGLCFTNKTSFEQFLAIDDKFTELENRYTNYLYNKQNENNLDKEVNDAIFAYQSFKNNHNLAFSTLQQELYNHEKLIKDYLTDPKLAVYKRNLMLVFRDKPHQLSSQTQSLLSQINPCFNQAERIFNILSTADLNLQPVVYQNKKYPINSVSDYQSLLENTNRGIRKACYEKWIEIY", "aa_hexdigest": "d2d2a3282c9a19d3b832092f49baa0e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25257.233700000015, "isoelectric_point": 8.274855613708496 }, "id": "KKNAAMEMLJ_404", "locus": "GCA000027325_00368" }, { "type": "cds", "sequence": "contig_1", "start": 209242, "stop": 209598, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATTACATCCAACTAGAGAACTTCGCTAAACTAAAGAACCATCCTAGTTACATTGCCCAAACTGCTTTTAATGATGAGATTGAAGTTGGTTTTATTGATTTTGTTTACCAACAAGTAGCTCAGTTTGCCAAAACCTTTCAAGCATTTATTCGCTTAAAAAAACAGATTTATAAACATGTTTTAAAAGTCAATAAAGTTGAACCATATGATCTTACCCTAACACTTTTTAAAACTAAAAAATCATACACGATTGAACAAGCTAAACAAGATGCACTAAAAGTTTTAGATCTACTTGGTGACAACTACATCAAGATAGTTAAAAAGGCTTTTAATGAAAACTGGATTGATTGA", "aa": "MENYIQLENFAKLKNHPSYIAQTAFNDEIEVGFIDFVYQQVAQFAKTFQAFIRLKKQIYKHVLKVNKVEPYDLTLTLFKTKKSYTIEQAKQDALKVLDLLGDNYIKIVKKAFNENWID", "aa_hexdigest": "d426fc31a6c30e1bef5a9e308927d37e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13956.981999999982, "isoelectric_point": 8.981558036804202 }, "id": "KKNAAMEMLJ_405", "locus": "GCA000027325_00369" }, { "type": "cds", "sequence": "contig_1", "start": 209674, "stop": 210318, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTTGATAAAACCAAATCATCACTAAATACGTTGGTACATGAACTTGGTCATTCAGTTCACTCTTGGTATGCATCACAACACCAATCCCAAAACATCGATCCTACTATCTTTTATGCTGAGATTGCTTCTATTGCCAATGAACTGTTGTTGTGTTATTATGAGCTGCAACTTTATAAAAATAACCACAAGCAGTTAATTGCTAGTTTATTGAGTCAAATCAACCATTTTTTTGGCGCTACTACAAGACAAATAATGTTTTCACAATTTGAAAAAGATACGCTTTATTTAATCAGAGTTAACCAGAAACCTGACTTTAAAACTTTGATTAAAATTTATGCAAATACTGCGGTTAAATACCAAGGTTTTAAACCTGAAGTAGTTGCTAATAAACTAAAAAAGACCCAGTATCAAAAATCATTGTCACACATCATTGCTATCCCCCATTTTTATGCAGGTAACTTCTATGTTTACAAGTATGCCATTGGCCAAGTTGCAGGTATTTTAGTAGCTAAAAAAATTAATAGTGGTGATAAAAAGATGAAAGATAATTACTTTAAATTCCTCAGTTCAGGTTCTAGTTTAGCGCCACTTGAAACCATTAAACTCTTAGGGATTGACCTTACTTCACCCCAACCCTGA", "aa": "MNFDKTKSSLNTLVHELGHSVHSWYASQHQSQNIDPTIFYAEIASIANELLLCYYELQLYKNNHKQLIASLLSQINHFFGATTRQIMFSQFEKDTLYLIRVNQKPDFKTLIKIYANTAVKYQGFKPEVVANKLKKTQYQKSLSHIIAIPHFYAGNFYVYKYAIGQVAGILVAKKINSGDKKMKDNYFKFLSSGSSLAPLETIKLLGIDLTSPQP", "aa_hexdigest": "2e829397f13d1898770a6730a78edf15", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24437.906799999997, "isoelectric_point": 9.473322868347168 }, "id": "KKNAAMEMLJ_406", "locus": "GCA000027325_00370" }, { "type": "cds", "sequence": "contig_1", "start": 210391, "stop": 211140, "strand": "+", "frame": 1, "gene": null, "product": "Type II methyltransferase M-MgeORF184P", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0032259", "SO:0001217", "UniRef:UniRef50_Q50290", "UniRef:UniRef90_Q49400" ], "nt": "GTGCACCATTTTAACCGCGCTAAAAAGGCCAAGAATAACGAGTTTTTTACTTTAATTGATGAGATTGAAAACGAAGTAATTAACTACCAAAAGCAGTTTGCAAATAAAACCATTTTTTGTAACTGTAATGATGGTAAAAATTCCCATTTTTTTCAGTTTTTTCAAACTAACTTTAACCAGTTACAACTAAAAAAACTCATTGGGTTTAGTTTTAATAATCTCTCACAAGCTGACAAGTTCACTTTTGATGGAAATAAAGTAACTAAAACCAAATTAAAGGGTAATGGTGATTTTAGTTCTGATGAATCGATTGAAGTGTTAAAACAAGCAGATATAGTTGTAACAAATCCACCCTTTAGTTTGTTTCAAAGTTTCATTGATCTGTTAATACAACACAACAAGCAGTTTCTGGTTTTAGGGTTAAATGCAGCAGTTAGCTATAACCATATTTTTACCTACTTTAAAACTAACAAGTTGTGGTTTGGCTATACCGTTAATAAAACAATGAGTTTTTCAGTTAACAGTGACTATCAACTTTATAACCCCAAAACTAGTAACTTTTTTACAAAAAATGGCAAGTGTTTCCAAAAGATAGCAGGTATCTCTTGGTTTACTAATTTAGGAAAACCACATTATAACCCCTTTTTAAATACCAACTGTTTTTATAAAAACAACGAAAAAAACTATCCCAAGTTTGATTGGTATGATGCTATTTATGTCAACAAGATTAAAAACATCCCTATGGATTGA", "aa": "MHHFNRAKKAKNNEFFTLIDEIENEVINYQKQFANKTIFCNCNDGKNSHFFQFFQTNFNQLQLKKLIGFSFNNLSQADKFTFDGNKVTKTKLKGNGDFSSDESIEVLKQADIVVTNPPFSLFQSFIDLLIQHNKQFLVLGLNAAVSYNHIFTYFKTNKLWFGYTVNKTMSFSVNSDYQLYNPKTSNFFTKNGKCFQKIAGISWFTNLGKPHYNPFLNTNCFYKNNEKNYPKFDWYDAIYVNKIKNIPMD", "aa_hexdigest": "cddcbbd39b52f9ffb7733348c1fcaccb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 210391, "stop": 211344, "observations": { "mutation": [ 211138 ], "stop": [ 211138 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49400", "query_cov": 1.0, "subject_cov": 0.7854889589905363, "identity": 1.0, "score": 513.0, "evalue": 2.74e-183, "gene_start": 1, "gene_end": 249, "reference_start": 1, "reference_end": 249, "reference_sequence": "MHHFNRAKKAKNNEFFTLIDEIENEVINYQKQFANKTIFCNCNDGKNSHFFQFFQTNFNQLQLKKLIGFSFNNLSQADKFTFDGNKVTKTKLKGNGDFSSDESIEVLKQADIVVTNPPFSLFQSFIDLLIQHNKQFLVLGLNAAVSYNHIFTYFKTNKLWFGYTVNKTMSFSVNSDYQLYNPKTSNFFTKNGKCFQKIAGISWFTNLGKPHYNPFLNTNCFYKNNEKNYPKFDWYDAIYVNKIKNIPMDWNGLMGVPLTFLNCYNPKQFELVDCLANPYATLDTLKTNAFVKLNQGDVRNVNGKRRYVRVIIKKQQI" }, "paralog": false, "identity": 0.9936908517350158, "score": 658.0, "evalue": 7.1e-243, "description": "Internal stop codon at 211138. Nonsense mutation around 211138.", "psc": { "uniref90_id": "UniRef90_Q49400", "product": "Type II methyltransferase M.MgeORF184P", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_Q50290", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49400", "EC:2.1.1.72", "UniRef:UniRef50_Q50290", "GO:0003677", "GO:0009007", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_Q50290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50290" ], "product": "Type II methyltransferase M.MpnI" } }, "id": "KKNAAMEMLJ_407", "locus": "GCA000027325_00371" }, { "type": "cds", "sequence": "contig_1", "start": 211150, "stop": 211344, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGTTCCTTTAACCTTTTTAAACTGTTACAACCCCAAGCAGTTTGAATTAGTTGATTGTCTTGCTAACCCTTATGCTACCTTAGATACATTAAAAACAAATGCCTTTGTGAAATTAAATCAGGGTGATGTGAGAAATGTTAATGGTAAAAGAAGGTATGTAAGGGTAATAATTAAAAAACAACAAATTTAG", "aa": "MGVPLTFLNCYNPKQFELVDCLANPYATLDTLKTNAFVKLNQGDVRNVNGKRRYVRVIIKKQQI", "aa_hexdigest": "19e9acc1ba819a4c2e570ba6152ca2bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7371.567700000002, "isoelectric_point": 9.757757377624511 }, "id": "KKNAAMEMLJ_408", "locus": "GCA000027325_00372" }, { "type": "cds", "sequence": "contig_1", "start": 211444, "stop": 212070, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCAAACTATTTTTTTTATCAAGCAGTGTGTTGGGTCCAATTGCCCTATTTACAACTGCTTGTAGTGCTGTTTATAGGTTTGATCAAGTTGATGATGGCAAGATTAAACTAGCAACTGTAACTTCAGCTTCCGCTAGTGGCTCGCTTACTACTATCATCAGTAAATATAATTCACAAAAAGATCCTAATGATTATCCAGTGGAACTGGTTTCACTTGATAGTAGGGGCAGTTATTCTAATGGCAAAAAGGATCTGCAAGCTAAACTGCTAGCTAAGGATAAAAATAACTTTTATAACCTTACTTTTAACTATAGTGATGTAGTTTCAATCCTCTCAAGAAGCCAGATGGAGTTGAGCTTTGATACGGTTGATACTAGTAATTTTGATCCTAGTTTTCTTAGTTTTAACAATAATATTTCCAATGTTAATCCAAATAGCATCTATGCTTTACCTGCTACTGTATCAGGTGAAGTTTTAGTTTTAAATGGACCGGTGTTACATTACATTTTAAGTAGTGCTAAAAAAGATAGTAACACCACCCTTTCAACCCATTCAGCTAGCAATAATAGCAATAAGGGAACAATGGTTGTTGCTAGTGATAGTGAAACATCAAGTTTATGA", "aa": "MKFKLFFLSSSVLGPIALFTTACSAVYRFDQVDDGKIKLATVTSASASGSLTTIISKYNSQKDPNDYPVELVSLDSRGSYSNGKKDLQAKLLAKDKNNFYNLTFNYSDVVSILSRSQMELSFDTVDTSNFDPSFLSFNNNISNVNPNSIYALPATVSGEVLVLNGPVLHYILSSAKKDSNTTLSTHSASNNSNKGTMVVASDSETSSL", "aa_hexdigest": "45ade32ed9956687bfb8a4a4a7b370fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22476.832100000014, "isoelectric_point": 6.079066658020018 }, "id": "KKNAAMEMLJ_409", "locus": "GCA000027325_00373" }, { "type": "cds", "sequence": "contig_1", "start": 212095, "stop": 212259, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTCAAACTAATGAAACCCAAGTTTTAAAAAGTAATTCATCAGAATCTAACCAAACCCAAGCTAGTGATACAGAGATTAAAAAGATTTGGGGTGATTATCAAGAAGTTGATGGAGGGTTGAAAAATTACACCTTTAAAGCTAGTGTTTTTAACAATTGA", "aa": "MNAQTNETQVLKSNSSESNQTQASDTEIKKIWGDYQEVDGGLKNYTFKASVFNN", "aa_hexdigest": "2a354eb9ad66692db4e9e73bce5190c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6060.453400000002, "isoelectric_point": 4.715101432800293 }, "id": "KKNAAMEMLJ_410", "locus": "GCA000027325_00374" }, { "type": "cds", "sequence": "contig_1", "start": 212365, "stop": 213549, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_Q50289", "UniRef:UniRef90_P47431" ], "nt": "TTGGAAAATGCCTTATATACTGCTTTATTTGCTTCTGGAAAAGCTGATTACAATAACTTTCTTTTTAACATCAAAAACCAGCGAATTAATTTCAGTAACTTTTTTAATAAATCCTCAACTGCATTTCAAAACCTTAAAACTATCTTTAACAGCTTCAAATCCTTAATTGATCAAAACGGTTTAATCTCAAATGCACACTTTAACACCCCAGTTAATGACTATGCTAAGTTTAACCAATTAGCTTTTTACACCTCTTCAACTGCACGCTTTCCCTATTCATTTGCAAGTGATAGTGTAAAGCGTTTAATAGTTAATGACAAGACAATTGAAAACAAAAACAATAAGAGTGTTTTTGAGGTTAATTTAAGTAGTGATAGTGATAACAACAGTAACCTAATTGGTACTGTATCACTGGAAAATAGTAAACAAGTTTCACTCTATGAAAAGCAAGTGGATAGTAATAAACAAATTGGTGTTGATGCTTTGTTAATCAAAGATGAAACTTTAATCAACCATCTTAAGAGTTTAAAATCGCAAGTTAGTGCAAAAAGTGCTAGTGAAACTTCCCAAACAAAACAAAACAAAACCTTTTTAGCATTTACAACTGTGAATGCAGATCAAAAAGCAATCTTTGATGTTGGTAAACTTAATGGCAAAACTGCCAAAATCATTATTAATGCTACTGAAACAACTAATGCTAAGATTAGCACCTTACAAGAAAAAGAGGCAATAGTTCTAAAAGCACCCCAACGCTTTGAGAGCACTGATCCATTTCCTATTGCTTTAGTGCAAGGTCCTAGTTTAATAGGGATCCATGCAAATGAAAGAGAGGATATTGAAACCAAAAAGTTTGTGAATTGGTATCTGAATACTAAAGTCCAATGGGAAGAAAATTCTATTAAAACTCCTGCAGAATATGTAGCTGATAAAGCTTCTTATCTTTTACCTTTTAAAAATAGGCTAAATAATACTAACAGTTACAATGAGTTTGTTAAAACTGCAGTTAGTCAGTTTGCTGATAAAAATGTAACTAAATTTGCAGAACCTGCTGATTTTTTATCCAACAAAGTACGTGATGGTGTTAAGAGTAATTTAAATGCTGCAATTAACAACCACAGCATTGATTTTGATAGTTTTATCAATGATTTAACAGACTATCTTGGTAGTGATGTTAAAAACATCTAA", "aa": "MENALYTALFASGKADYNNFLFNIKNQRINFSNFFNKSSTAFQNLKTIFNSFKSLIDQNGLISNAHFNTPVNDYAKFNQLAFYTSSTARFPYSFASDSVKRLIVNDKTIENKNNKSVFEVNLSSDSDNNSNLIGTVSLENSKQVSLYEKQVDSNKQIGVDALLIKDETLINHLKSLKSQVSAKSASETSQTKQNKTFLAFTTVNADQKAIFDVGKLNGKTAKIIINATETTNAKISTLQEKEAIVLKAPQRFESTDPFPIALVQGPSLIGIHANEREDIETKKFVNWYLNTKVQWEENSIKTPAEYVADKASYLLPFKNRLNNTNSYNEFVKTAVSQFADKNVTKFAEPADFLSNKVRDGVKSNLNAAINNHSIDFDSFINDLTDYLGSDVKNI", "aa_hexdigest": "62b80a0f76a80f32b0c82993100703fd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 212071, "stop": 213549, "observations": { "mutation": [ 212257 ], "stop": [ 212257 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47431", "query_cov": 1.0, "subject_cov": 0.5620542082738944, "identity": 0.997, "score": 712.0, "evalue": 2.57e-253, "gene_start": 1, "gene_end": 394, "reference_start": 308, "reference_end": 701, "reference_sequence": "MKFKLFFLSSSVLGPIALFTTACSAVYRFDQVDDGKIKLATVTSASASGSLTTIISKYNSQKDPNDYPVELVSLDSRGSYSNGKKDLQAKLLAKDKNNFYNLTFNYSDVVSILSRSQMELSFDTVDTSNFDPSFLSFNNNISNVNPNSIYALPATVSGEVLVLNGPVLHYILSSAKKDSNTTLSTHSASNNSNKGTMVVASDSETSSLWTKLEAAAKMNAQTNETQVLKSNSSESNQTQASDTEIKKIWGDYQEVDGGLKNYTFKASVFNNWKDLNDFATRIAKSFTKLQTTTKKGEEVQAVFGIGSLENALYTALFASGKADYNNFLFNIKNQRINFSNFFNKSSTAFQNLKTIFNSFKSLIDQNGLISNAHFNTPVNDYAKFNQLAFYTSSTARFPYSFASDSVKRLIVNDKTIENKNNKSVFEVNLSSDSDNNSNLIGTVSLENSKQVSLYEKQVDSNKQIGVDALLIKDETLINHLKSLKSQVSAKSASETSQTKQNKTFLAFTTVNADQKAIFDVGKLNGKTAKIIINATETTNAKISTLQEKEAIVLKAPQRFESTDPFPIALVQGPSLIGIHANEREDIETKKFVNWYLNTKVQWEENSIKTPAEYVADKASYLLPFKNRLNNTNSYNEFVKTAVSQFADKNVTKFAEPADFLSNKVRDGVKSNLNAAINNHSIDFDSFINDLTDYLGSDVKNI" }, "paralog": false, "identity": 0.9471544715447154, "score": 861.0, "evalue": 1.86e-314, "description": "Internal stop codon at 212257. Nonsense mutation around 212257.", "psc": { "uniref90_id": "UniRef90_P47431", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_Q50289", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47431", "UniRef:UniRef50_Q50289", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_Q50289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50289" ], "product": "Uncharacterized lipoprotein MPN_199" } }, "id": "KKNAAMEMLJ_411", "locus": "GCA000027325_00375" }, { "type": "cds", "sequence": "contig_1", "start": 213585, "stop": 213935, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTAAGGTGGTAGTGGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAATCTTCTTCCTCTTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCGGTGAAGGATTCAAACAAAGACAGTGAGAAACTCGAAACAACTGCTTCATCGATGAGTAGTGGGGCTACATCTCCTCGCAAGGCCCTTAAGATAGAGGTGGAGAAAGGCAGTAATGTCAATCAAGGCGAACTAGCAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAGAACAGTAGTGGGACAGAGGTGAAGTTAGCTGCGAATGGGGAGTTTGCAGGAGACAAGGCCTGA", "aa": "MPKVVVEYHNLNNQVVKESLEVESSSSFNPTQRLQKDSPVKDSNKDSEKLETTASSMSSGATSPRKALKIEVEKGSNVNQGELAKNDFAKKPLKHKNSSGTEVKLAANGEFAGDKA", "aa_hexdigest": "114202d0cfccf460b0fb1faaa1c5df66", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030CD7D5B", "query_cov": 0.9655172413793104, "subject_cov": 0.8484848484848485, "identity": 0.795, "score": 153.0, "evalue": 5.73e-46, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12547.812099999985, "isoelectric_point": 8.804012107849122 }, "id": "KKNAAMEMLJ_412", "locus": "GCA000027325_00376" }, { "type": "cds", "sequence": "contig_1", "start": 214200, "stop": 214571, "strand": "+", "frame": 3, "gene": null, "product": "Random genomic clone esh10, partial cds", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "nt": "GTGAAACCTAGCAATAGTAGTCAACAGTTTAATCCCAATTCAGATGATAATAAAGTCACTCAAGGTGGTGGCTCCCCAGCCAAAAAAACAACGTATGACAATTTACCAAACTCCATCAGTCCCACCAGTGACTGGATCAACGCATTGACTTTTACTAATAAGAATAACCCGCAGCGCAATCAACTGTTGCTCAGAAGCTTACTAGGAACTATCCCGGTCTTGATCAATAAGAGTGGAGGAAGTGGGAATGAGTTTACCCATACGAGTGAGCAGAAGTGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTATTATGTTTTTATCAAATCTTTATTTACTTTAA", "aa": "MKPSNSSQQFNPNSDDNKVTQGGGSPAKKTTYDNLPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGGSGNEFTHTSEQKWDKTNEKDGNLPGFGEVNGGFIMFLSNLYLL", "aa_hexdigest": "5e30b77c0bcb35cf72672fb67e05a4cd", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D20171", "query_cov": 0.926829268292683, "subject_cov": 0.9661016949152542, "identity": 0.851, "score": 188.0, "evalue": 6.69e-60, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_Q49206" }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" }, "genes": [], "id": "KKNAAMEMLJ_413", "locus": "GCA000027325_00377" }, { "type": "cds", "sequence": "contig_1", "start": 214975, "stop": 215142, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI002FCE54A6" ], "nt": "GTGAAAAAGTATCAGTTAGATCTACCAAATGTTACCAATGAGATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCCCTTACCAAGTATACCTTAAACAGTAGTTTGGCTAAACAAAAGGGTAAAACCCAAAGGGAGGTGCATCTCTCAAATTCAAATCAGTGA", "aa": "MKKYQLDLPNVTNEMNQVLQELILEQPLTKYTLNSSLAKQKGKTQREVHLSNSNQ", "aa_hexdigest": "f5b73ee8d3f20c77b10343c7b62bfafb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 214828, "stop": 215207, "observations": { "deletion": [ 215158 ], "mutation": [ 214966, 215140 ], "stop": [ 214966, 215140, 215164 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE54A6", "query_cov": 0.9090909090909091, "subject_cov": 0.4032258064516129, "identity": 0.88, "score": 86.3, "evalue": 1.54e-20, "gene_start": 1, "gene_end": 50, "reference_start": 48, "reference_end": 97, "reference_sequence": "MLFKSTQVKLTYSSSGSNNISFENNANKPSYVVEFTNSTTVGIKWSVVKKYQLDVPNVTNEMNQVLQELILEQPLTKYTLNGSLAKQKGKSQVAVHLGGQASQWHSTRHSQSKQQSQPQGFNRI" }, "paralog": false, "identity": 0.7244094488188977, "score": 158.0, "evalue": 1.17e-51, "description": "Internal stop codon at 214966, 215140, 215164. Frameshift due to deletion around 215158. Nonsense mutation around 214966, 215140.", "psc": { "uniref90_id": "UniRef90_UPI002FCE54A6", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE54A6", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6386.229300000001, "isoelectric_point": 9.158008003234862 }, "id": "KKNAAMEMLJ_414", "locus": "GCA000027325_00378" }, { "type": "cds", "sequence": "contig_1", "start": 215270, "stop": 215422, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACAGAAGCAACAACGGCAAAAAATGATGCGCCCAGTGTTTCTGGAAGTGGAACATCAGACACCGCTTCAAAATTCAAAAGTTACCTCAACACCAAGCAAGCGTTAG", "aa": "MGPSRAKGRIVVGGVQQKQQRQKMMRPVFLEVEHQTPLQNSKVTSTPSKR", "aa_hexdigest": "9c3a715692413b01a295a52d9161efcf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5660.5650000000005, "isoelectric_point": 11.849047279357908 }, "id": "KKNAAMEMLJ_415", "locus": "GCA000027325_00379" }, { "type": "cds", "sequence": "contig_1", "start": 215444, "stop": 215881, "strand": "+", "frame": 2, "gene": null, "product": "Adhesin-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49384", "UniRef:UniRef90_Q49384" ], "nt": "ATGGAACAACGGCGAGGAATGTGGTTACCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAAGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCGAGCAGCATGACCAACTTGTTAAACATGTTGTCTAGTAAAAACATCAAACAGAAGTTGGGGAAGGGGGGAACAGCAATGCAGGGAAGTTTCAGTGTCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATACAAATAGTGGAACAACTGGACCCATTAAAACTGCTTATCCGGTGAAAAAAGATCAAAAATCAACTGTCAAGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATTTAAATAATCACACCAAGTTAATTTACCCAAATTATTTACTTATTTATTAA", "aa": "MEQRRGMWLPSFLYFQPNKVKSGQYQQNNTYNKLIEPESATSAASSMTNLLNMLSSKNIKQKLGKGGTAMQGSFSVQDTFSFVVPYSGNHTNSGTTGPIKTAYPVKKDQKSTVKINSLINATPLNSYGDLNNHTKLIYPNYLLIY", "aa_hexdigest": "bd23f25ec36e2a8f2de79c044147f793", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49384", "query_cov": 1.0, "subject_cov": 1.0, "identity": 1.0, "score": 286.0, "evalue": 7.46e-98, "valid": true, "product": "Adhesin-like protein", "uniref50_id": "UniRef50_Q49384" }, "pscc": { "uniref50_id": "UniRef50_Q49384", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49384" ], "product": "Adhesin-like protein" }, "genes": [], "id": "KKNAAMEMLJ_416", "locus": "GCA000027325_00380" }, { "type": "cds", "sequence": "contig_1", "start": 216016, "stop": 216174, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGGTTTTTAAAACCAAATTTCTCGCTCGGTGCTTTGTTTTTAACTTTAAGCCCCATAGCCACTGCATGCATTGCTGAAAAACCAGTTAACAACCGCTTTAACTTTAATAGCGAGCAATTAGCTAGGCTAAGAAAAGCAAGGGTTAACCACTGA", "aa": "MKGFLKPNFSLGALFLTLSPIATACIAEKPVNNRFNFNSEQLARLRKARVNH", "aa_hexdigest": "ba96a79688dc3cbf84dc2154b7a16318", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5845.803, "isoelectric_point": 11.006509971618652 }, "id": "KKNAAMEMLJ_417", "locus": "GCA000027325_00381" }, { "type": "cds", "sequence": "contig_1", "start": 216190, "stop": 216366, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGTTAGCTTTGCAAATAACCACCAAAAACCGATCCGTATCTATGCCATTGATACCCCTGAAAAAGCAGTTTTATCTATACAACGCAAATCAGAGATAGAACTTAAAGAAGCTAATAAAGCAACTGAGTTTGCCAAAAGCTTAATTCCCATTGGTAGTGAGGTGTGGATCTGA", "aa": "MEVSFANNHQKPIRIYAIDTPEKAVLSIQRKSEIELKEANKATEFAKSLIPIGSEVWI", "aa_hexdigest": "5b63f637902c862a17c023ab7011c0ee", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6583.523299999997, "isoelectric_point": 6.557253074645996 }, "id": "KKNAAMEMLJ_418", "locus": "GCA000027325_00382" }, { "type": "cds", "sequence": "contig_1", "start": 216406, "stop": 216678, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418B8", "UniRef:UniRef90_UPI0001B418B8" ], "nt": "GTGTTTTTCAAAACCAATCCATTGCAACTGCACTTTGAATCGTTTGCAGTTGAAATGGTAGCAAATGGTCATGCTTTACCTATTGCTGGTAATGACTTTGATTTTGTTTTTAGTGATTTGGACCCATTTAATCCCCTAAAAATAGTAGGGATTGAACTAGCTAATGGTTTAAACAATGCTTTTAACAACAGAAAAAACATCTTTAGTTATTTAGAAAACAGTTTTCAATCAATAACAATGGTCTATCAACAACGCGGTGTTGACCAAAGTTGA", "aa": "MFFKTNPLQLHFESFAVEMVANGHALPIAGNDFDFVFSDLDPFNPLKIVGIELANGLNNAFNNRKNIFSYLENSFQSITMVYQQRGVDQS", "aa_hexdigest": "240d5fe9c49f5fc1a8f13eb332e8c5a7", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 216355, "stop": 216768, "observations": { "mutation": [ 216364, 216676 ], "stop": [ 216364, 216676 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B418B8", "query_cov": 1.0, "subject_cov": 0.656934306569343, "identity": 0.9890000000000001, "score": 179.0, "evalue": 1.02e-56, "gene_start": 1, "gene_end": 90, "reference_start": 18, "reference_end": 107, "reference_sequence": "MWIWPLNSYSYDREVAAVFFKTNPLQLHFESFAVEMVANGHALPIAGNDFDFVFSDLDPFNPLKIVGIELANGLNNAFNNRKNIFSYLENSFQSITMVYQQRGVDQSWTRYLAPSNDFSSTKLGLGLTIYELKLNNG" }, "paralog": false, "identity": 0.9781021897810219, "score": 270.0, "evalue": 4.68e-95, "description": "Internal stop codon at 216364, 216676. Nonsense mutation around 216364, 216676.", "psc": { "uniref90_id": "UniRef90_UPI0001B418B8", "uniref50_id": "UniRef50_UPI0001B418B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B418B8", "UniRef:UniRef50_UPI0001B418B8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B418B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418B8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10206.387099999996, "isoelectric_point": 4.7775674819946286 }, "id": "KKNAAMEMLJ_419", "locus": "GCA000027325_00383" }, { "type": "cds", "sequence": "contig_1", "start": 216761, "stop": 217090, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF03215.21" ], "nt": "ATGGCTAACAATAAGAGTGCAATTGAGTTGAAAAACATCGTTGTTGATTTTGGTGAATCAGTTGCGATTGACAACATTAACCTTAGTGTTGAAAAACACCAACTAGTTAGCTTACTTGGTCCTAGTGGTTGTGGTAAAACCACTACACTTGCAGTTATTGCAGGACTTATTAAACCAACTAGTGGTCAGGTGTTATTTAATGGTTATGATGTCACCAAAAAACCACCCCAAGAACGTAAACTAGGGCTAGTTTTTCAAAACTATGCACTTTATCCGCACATGAATGTGTTTGAAAACATTGTTTTCCCCCTCTACAGTGATAACTCGTGA", "aa": "MANNKSAIELKNIVVDFGESVAIDNINLSVEKHQLVSLLGPSGCGKTTTLAVIAGLIKPTSGQVLFNGYDVTKKPPQERKLGLVFQNYALYPHMNVFENIVFPLYSDNS", "aa_hexdigest": "b932d116271233ef4cdc0f6ca5d63cc5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034E961FD", "query_cov": 0.8440366972477065, "subject_cov": 0.8679245283018868, "identity": 0.5329999999999999, "score": 114.0, "evalue": 9.31e-31, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A3B9G3L7" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 78, "aa_cov": 0.6972477064220184, "hmm_cov": 1.0, "evalue": 2.895139844567465e-21, "score": 71.6120834350586, "start": 24, "stop": 99 }, { "id": "PF03215.21", "name": "Rad17", "length": 79, "aa_cov": 0.7155963302752294, "hmm_cov": 0.9873417721518988, "evalue": 4.4321896171629685e-08, "score": 28.33935546875, "start": 23, "stop": 100 } ], "seq_stats": { "molecular_weight": 11895.559399999991, "isoelectric_point": 6.710377311706543 }, "id": "KKNAAMEMLJ_420", "locus": "GCA000027325_00384" }, { "type": "cds", "sequence": "contig_1", "start": 217103, "stop": 217396, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAAAACAGTGTTGCAAACCATGAGATTAACTGTTTGTTACTTACTAGCAACGGTGCATCAGTTCAAGAGATTGATCAGCTCAATAAGTTATTTCATGATAGTATTGAAAAACCCAAACAGATCCAATACCAAATTAATGACCTTAATGTTAGTGTTTTTAAAAACTTAAATGAACTAACTGCAAACCTTAAGTTAATACCAAGTAAGCACCAGTTTGCTATTACCAATCTCAACAAACAAACTCTAAAACAGATTAATGAACTGGAAGCTGAGTTTAAAACAAAGTGA", "aa": "MEKNSVANHEINCLLLTSNGASVQEIDQLNKLFHDSIEKPKQIQYQINDLNVSVFKNLNELTANLKLIPSKHQFAITNLNKQTLKQINELEAEFKTK", "aa_hexdigest": "df1742a48338dccf1a178445687dc124", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11119.565499999986, "isoelectric_point": 6.824282646179199 }, "id": "KKNAAMEMLJ_421", "locus": "GCA000027325_00385" }, { "type": "cds", "sequence": "contig_1", "start": 217517, "stop": 218221, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGGAACTAAAACAAACCCTTGTTGAAAACCTTAAGTTAGTTAAAAAAGCGATTAGTGAACAAACTAAGTTAATTAAACAGAGTAGTGATTACACTAAGTTAAAGCAATTAAAACGGTTAATTAAAGTTGAACCTAACCAACTCAAAAAACAATATAAGGTTTTTCTCAATCAGTTAATTAAAAACTATTCACTTAAAACTGATAAGTTAACTGATACTCAACTTAATGAAATTGAACAGATTAAAACCAGAATTGTTTCAATAAAACAGTTTATCAACAAAACTGCACTTGAAGTAGCTAACAAACTAGCGATTACCAAGATTTTAACCAAACGCCCTGATAAGATTTCTGGTGGACAACAACAACGCGTAGCAATTGCTAGAGCAATTGTCAGAAGACCTAAACTATTGTTAATGGATGAACCACTCTCTAACTTAGATGCAAAGCTAAGGGTACAGACAAGACAGTGGATCAGACAGTTTCAACAGGAGTTACAAATTACCACTGTTTTTGTCACCCATGACCAGGAAGAAGCGATGAGTATTAGTGATGTCATTGTTTGTATGTCAACTGGAAAAGTGCAGCAAATCGGCACACCCAGTGAACTTTATTTAAAACCTGCTAATGAGTTTGTTGCGCGCTTTTTAGGCACCCCTGAGATGAACATCATTGAATGTAGTGTCAAAAACAACCAGTTGTTTTGA", "aa": "MELKQTLVENLKLVKKAISEQTKLIKQSSDYTKLKQLKRLIKVEPNQLKKQYKVFLNQLIKNYSLKTDKLTDTQLNEIEQIKTRIVSIKQFINKTALEVANKLAITKILTKRPDKISGGQQQRVAIARAIVRRPKLLLMDEPLSNLDAKLRVQTRQWIRQFQQELQITTVFVTHDQEEAMSISDVIVCMSTGKVQQIGTPSELYLKPANEFVARFLGTPEMNIIECSVKNNQLF", "aa_hexdigest": "35f563f66be9cc9453b00407e146a237", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 143, "aa_cov": 0.5854700854700855, "hmm_cov": 0.7692307692307693, "evalue": 6.70905610294915e-14, "score": 47.75909423828125, "start": 7, "stop": 143 } ], "seq_stats": { "molecular_weight": 27008.450900000003, "isoelectric_point": 9.8450475692749 }, "id": "KKNAAMEMLJ_422", "locus": "GCA000027325_00386" }, { "type": "cds", "sequence": "contig_1", "start": 218300, "stop": 218518, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTCACTACTAACAAAAGTAGTTTGCAAGCTAAACTAATTAACATTGAAAACTTAGGTAAACACTTAGTTGCTACCATTAGTTTGTTTGATACCACCTTATCAATGCGCTTAGAATTGAATAGCCACTTAAAAGTAGGTGATAGTTTAAATTTCATTATTAAAGCTAACAACCTCCATTTTTTTGATATTGATACAAAACAACGGATTGAGATTTAA", "aa": "MVTTNKSSLQAKLINIENLGKHLVATISLFDTTLSMRLELNSHLKVGDSLNFIIKANNLHFFDIDTKQRIEI", "aa_hexdigest": "e837cd6c9e16c876478aabd4345a93c9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8168.404699999995, "isoelectric_point": 8.158812522888184 }, "id": "KKNAAMEMLJ_423", "locus": "GCA000027325_00387" }, { "type": "cds", "sequence": "contig_1", "start": 218614, "stop": 218895, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCCTCCCTGCGCTTTTAACAACAATTTTGTTTACCATTATCCCCTTCTTTTTAAGCTTACAGAAGGGTTTTAGTGCTAACAGTGATCTGTATGATCTCTCCTCCCAATCCTTTAGTTTACGAACCTTTCAGGATCTGTTTAGTGAATCTAACTTTGTGTTGGGCTTACGCAATAGTTTTCTCTATTCACTAATCTCTTTACCCTTTAGCATTATCATTGCTATTGTTATTGCCAGTGCCATAGTATTTGTGTACAAAAAATTGTTAAGAGGGTTTTGA", "aa": "MFLPALLTTILFTIIPFFLSLQKGFSANSDLYDLSSQSFSLRTFQDLFSESNFVLGLRNSFLYSLISLPFSIIIAIVIASAIVFVYKKLLRGF", "aa_hexdigest": "6348c8270d0f9353081b6a4d9b998a0a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10499.301899999988, "isoelectric_point": 9.30054759979248 }, "id": "KKNAAMEMLJ_424", "locus": "GCA000027325_00388" }, { "type": "cds", "sequence": "contig_1", "start": 218902, "stop": 219465, "strand": "+", "frame": 1, "gene": "ugpA", "product": "putative ABC transporter permease protein MG188", "db_xrefs": [ "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75263", "UniRef:UniRef90_P47434" ], "nt": "GTGTTTTTTTTACCCTATGTAACTTCAGGGGTGGCAATCTCGATTGCCTTTGTATATATCTTTGATTCTGCCTCTGGTATTTTAAACACGGTGTTTAATGTCAACACCAAGTGGCTTGATTCAGGTTCACGTGATACATTTAATGCCTTGTGGGCTATCTTAATCTTTGGGGTGTGGAAAAACTTGGCATTTAATGTGTTGATTATCTCAACAGCAATGTTAAGTGTTAATCCCCAACTTTACAAGGTAGCAAGTTTAGATAGTGCCAATCCTGTAAGACAGTTCTTTAAGATTACTCTTCCCTCCATCCGTCCTACTTTAATCTTTCTTACTACCCTTTTAATCTTAGGGGGGATGCAAGTCTTTCCGCTGGCTTTGTTTGAAAACAAACCTGAAGAAGCGGTTGCTAATGGGGGGAATAGTATCTTGCTTTACATCTTTCAACAGATCCAAAGTGGCAATACTAACTTAGCAGGTGCTGCTACTTTAGTGTTGTTTGTGTTGGGAGTTTGTTATGGGTTAGTGTTACGTAATGGCTTTTATCTGATTGAATGGTTGCAGTGA", "aa": "MFFLPYVTSGVAISIAFVYIFDSASGILNTVFNVNTKWLDSGSRDTFNALWAILIFGVWKNLAFNVLIISTAMLSVNPQLYKVASLDSANPVRQFFKITLPSIRPTLIFLTTLLILGGMQVFPLALFENKPEEAVANGGNSILLYIFQQIQSGNTNLAGAATLVLFVLGVCYGLVLRNGFYLIEWLQ", "aa_hexdigest": "23a383ef9e846a29711dce8de3158b3a", "start_type": "GTG", "rbs_motif": null, "genes": [ "ugpA" ], "truncated": "both", "pseudogene": { "start": 218602, "stop": 219510, "observations": { "mutation": [ 218893, 219463 ], "stop": [ 218893, 219463 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47434", "query_cov": 1.0, "subject_cov": 0.5683890577507599, "identity": 0.995, "score": 359.0, "evalue": 3.84e-123, "gene_start": 1, "gene_end": 187, "reference_start": 128, "reference_end": 314, "reference_sequence": "MFKWLLKHHNQPHSLQLGLLDQPLPFWKPFLLFLPALLTTILFTIIPFFLSLQKGFSANSDLYDLSSQSFSLRTFQDLFSESNFVLGLRNSFLYSLISLPFSIIIAIVIASAIVFVYKKLLRGFWQTVFFLPYVTSGVAISIAFVYIFDSASGILNTVFNVNTKWLDSGSRDTFNALWAILIFGVWKNLAFNVLIISTAMLSVNPQLYKVASLDSANPVRQFFKITLPSIRPTLIFLTTLLILGGMQVFPLALFENKPEEAVANGGNSILLYIFQQIQSGNTNLAGAATLVLFVLGVCYGLVLRNGFYLIEWLQWKIKQLYVQKQLTLY" }, "paralog": false, "identity": 0.9933774834437086, "score": 577.0, "evalue": 9.52e-212, "description": "Internal stop codon at 218893, 219463. Nonsense mutation around 218893, 219463.", "psc": { "uniref90_id": "UniRef90_P47434", "gene": "UgpA", "product": "Probable ABC transporter permease protein MG188", "uniref50_id": "UniRef50_P75263", "cog_id": "COG1175", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47434", "UniRef:UniRef50_P75263", "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75263" ], "product": "Probable ABC transporter permease protein MG188 homolog" } }, "id": "KKNAAMEMLJ_425", "locus": "GCA000027325_00389" }, { "type": "cds", "sequence": "contig_1", "start": 220010, "stop": 220165, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCTTTGTTAGCAGTGTTTTTAGTGGGTTTATGTACCGTACTAGCTTTGAAGCCATTCCATCTCAATTAAAAGAGTCAGCACTCATTGATGGGTGTAATGGCTTTAACTACTTTTTGAAGATTGCTTTACCAATGGTGAAATCTACCAGTTGA", "aa": "MPFVSSVFSGFMYRTSFEAIPSQLKESALIDGCNGFNYFLKIALPMVKSTS", "aa_hexdigest": "085b3f2dbe63731aa5df8fccf6c2c25d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5658.5434, "isoelectric_point": 7.899262809753418 }, "id": "KKNAAMEMLJ_426", "locus": "GCA000027325_00390" }, { "type": "cds", "sequence": "contig_1", "start": 220238, "stop": 220438, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTAAACATTAACTTGTGGGTGTTACAACAAGGGATCTTGGATGCTAACAGTAGTGATGAACAGATCAGAACGCTGTTAAATCTCAAGATGAGTGCAGCGATTCTAGCTATCCTTCCGATGTTTATTATCTACTTTTTGTTCCATAAAAGGATTATGAATGCCATTAAAAACAGAGCCAACACCATTAAGGGTTAA", "aa": "MDLNINLWVLQQGILDANSSDEQIRTLLNLKMSAAILAILPMFIIYFLFHKRIMNAIKNRANTIKG", "aa_hexdigest": "4b4661cc8f13f3dff15fd38d9d37aa1a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7572.9792999999945, "isoelectric_point": 9.817390632629394 }, "id": "KKNAAMEMLJ_427", "locus": "GCA000027325_00391" }, { "type": "cds", "sequence": "contig_1", "start": 220401, "stop": 220598, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D36BE5", "UniRef:UniRef90_UPI0002D36BE5" ], "nt": "ATGCCATTAAAAACAGAGCCAACACCATTAAGGGTTAATATGCAAAAGTTTAAACAACTGGTTGGTGCAATGCACAGATGGGTAAAACTAGCACTATTAGTAATCATTGTGTTATTAGGGATTATCTTTTGTCTGTTTGCCATCTATGACATTGCGCAAGTGATCATTACCATTATCAATGAAGGGGCACTTTTATAA", "aa": "MPLKTEPTPLRVNMQKFKQLVGAMHRWVKLALLVIIVLLGIIFCLFAIYDIAQVIITIINEGALL", "aa_hexdigest": "b3c85ec9a08def8a2bd7c629d8694a61", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002D36BE5", "query_cov": 0.8, "subject_cov": 1.0, "identity": 0.981, "score": 89.4, "evalue": 2.08e-22, "valid": true, "uniref50_id": "UniRef50_UPI0002D36BE5" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D36BE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D36BE5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7346.055099999994, "isoelectric_point": 9.514131355285645 }, "id": "KKNAAMEMLJ_428", "locus": "GCA000027325_00392" }, { "type": "cds", "sequence": "contig_1", "start": 220607, "stop": 221374, "strand": "+", "frame": 2, "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.-.-", "GO:0003676", "GO:0004527", "GO:0008441", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_P75144", "UniRef:UniRef90_P22746" ], "nt": "ATGAAAAAAGGATCAATAACTGAAGCAATTAATGCCATTAAACAATTTGATAAGATTGTTATCTTTCACCATGTGCGCCCTGATGGGGATTGTTTAGGAGCACAACAAGGCTTGTTTCACCTCATTAAAGCTAACTTTAAAAATAAGGAGGTGAAGTGTGTTGGTAATAACAACAACCTGTTTAGCTTTATCAACATGACATTTACCAACCAAATTGATGAGAGCTTTTTAAAAGAAGCACTTGCCATTGTGGTCGATGCTAATTACAAAAACAGGATTGAATTGAGAGAACTGTTAGATAAAAACCTGTTTAAAGCAGTGTTAAGGATTGATCACCATCCCAATGAAGATGATCTAAACACTAGCTTTAACTTTGTTGAAGAAAGCTATGTAGCTTGTTGTGAGCAGATAGTGGAGATGGCCACAGTGGCGAAGTGGACCATACCACCAGTGGCTGCTACTTTACTATATATAGGTATCTATACGGATAGTAATAGATTTCTATATAGTAATACATCATATAGAACACTATACTTAGCAGCAATACTATATAAAGCTAAAGCTGATATAAGGATAGTACATGATCATTTAAACCATACTAGTTTAGCAGATCTTAAGTTTAAAAAGTATGTTTATAACCACTTTAAAACCCAAGGACAAGTGATCTATTTTATCTGTACTAAAAAGATCCAAAAGAGACTAAGAATGACTGCAGATCAATGTGCTAGAGTTAACTTGTTAAGTAACATAGCAGATTACAAGATCTGA", "aa": "MKKGSITEAINAIKQFDKIVIFHHVRPDGDCLGAQQGLFHLIKANFKNKEVKCVGNNNNLFSFINMTFTNQIDESFLKEALAIVVDANYKNRIELRELLDKNLFKAVLRIDHHPNEDDLNTSFNFVEESYVACCEQIVEMATVAKWTIPPVAATLLYIGIYTDSNRFLYSNTSYRTLYLAAILYKAKADIRIVHDHLNHTSLADLKFKKYVYNHFKTQGQVIYFICTKKIQKRLRMTADQCARVNLLSNIADYKI", "aa_hexdigest": "c61dc6f49646d92cd8320888303b3198", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P22746", "query_cov": 1.0, "subject_cov": 0.8018867924528302, "identity": 1.0, "score": 512.0, "evalue": 1.47e-182, "valid": true, "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75144", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "go_ids": [ "GO:0008441", "GO:0004527", "GO:0003676" ] }, "pscc": { "uniref50_id": "UniRef50_P75144", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75144" ], "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA" }, "genes": [ "nrnA" ], "id": "KKNAAMEMLJ_429", "locus": "GCA000027325_00393" }, { "type": "cds", "sequence": "contig_1", "start": 222011, "stop": 222532, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "ATGGAAAACCCCTCAACGGTGCAAAGGGGTTTAAATGGCGAGCCTATCTTTGATCCTTTTAAAGGCTTTGGTTTAACTGGTAATGCCCCTACTGATTGGAATGAGATCAAAGGTAAAGTTCCAGTAGAAGTAGTTCAATCCCCCCATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGCATTAGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGAAAGCAACCCAATCATCCTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCAGTGAAGGATTCAAGTAAACAAGGGGAGAAACTCAGTGAAACAACTGCTTCATCCATGAGTAGTGGTATGGCTACATCCACTCGAGCCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCACTAAAGCATAAGAACAGTAGTGGGGAGGTGAAGTTAGAGGCAGAGAAGGAGTTTACTGAGGCCTGA", "aa": "MENPSTVQRGLNGEPIFDPFKGFGLTGNAPTDWNEIKGKVPVEVVQSPHSPNLYFVLLVPKVALEYHNLNNQVVKESLEVKATQSSFNPTQRLQKDSPVKDSSKQGEKLSETTASSMSSGMATSTRAKALKVEVERGSQSDSLLKNDFAKKPLKHKNSSGEVKLEAEKEFTEA", "aa_hexdigest": "58fbcfda93e3fc6c2193b8d31fa5bcf4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 76, "aa_cov": 0.4393063583815029, "hmm_cov": 1.0, "evalue": 6.183651571095011e-19, "score": 63.48705291748047, "start": 9, "stop": 84 } ], "seq_stats": { "molecular_weight": 18975.1096, "isoelectric_point": 7.883210182189941 }, "id": "KKNAAMEMLJ_430", "locus": "GCA000027325_00394" }, { "type": "cds", "sequence": "contig_1", "start": 222542, "stop": 222691, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGACTACTGATCAAATAGCAAGAGAGAAGGGGATGGGGGCGACGGTGGTTAGTTTCTATGATGCACCCTACAGTGAAAACCATACTGCCTTTGGACTTGTTGATCACATCGATCCTAAAAAGATGGTTGAAAACTACCCACCAAGTTGA", "aa": "MTTDQIAREKGMGATVVSFYDAPYSENHTAFGLVDHIDPKKMVENYPPS", "aa_hexdigest": "2f998f47c386bd580846a6925261eb39", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 50, "aa_cov": 0.9795918367346939, "hmm_cov": 1.0, "evalue": 8.553836149263662e-12, "score": 40.0535774230957, "start": 2, "stop": 49 } ], "seq_stats": { "molecular_weight": 5460.048300000002, "isoelectric_point": 4.960191154479979 }, "id": "KKNAAMEMLJ_431", "locus": "GCA000027325_00395" }, { "type": "cds", "sequence": "contig_1", "start": 222746, "stop": 223078, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGTTACAAACAACAGGGTTCTTTAACCCAAGAAGACACCCGGAGTGGTTTGATGAAGGACAAGCTAAGGCAGATAACACTAGCCCTGGCTTTAAGGTAGGGGATACTGATCACAAAAAAGACGGGTTTAAAAAAAACTCTTCTTCTCCAATAGCTTTACCATTTGAAGCATACTTTGCTAACATTGGTAACATGGTTGCTATTGGTAACTCGGTATTTATCTTTGGTGGTAATGGTCATGCTACTAAGATGTTTACCACCAATCCCTTAAGTATTGGGGTATTTAGGATTAAATACACTGATAACTTTAGTAAGTCATCAGTAACAGGTTGA", "aa": "MLQTTGFFNPRRHPEWFDEGQAKADNTSPGFKVGDTDHKKDGFKKNSSSPIALPFEAYFANIGNMVAIGNSVFIFGGNGHATKMFTTNPLSIGVFRIKYTDNFSKSSVTG", "aa_hexdigest": "4897f06be76cbd6d3cc4f1a5c57ee6d1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 107, "aa_cov": 0.9727272727272728, "hmm_cov": 0.794392523364486, "evalue": 2.8996640279179817e-34, "score": 113.79003143310547, "start": 4, "stop": 110 } ], "seq_stats": { "molecular_weight": 12057.375399999988, "isoelectric_point": 9.34657802581787 }, "id": "KKNAAMEMLJ_432", "locus": "GCA000027325_00396" }, { "type": "cds", "sequence": "contig_1", "start": 223088, "stop": 223360, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGTTATTTGGGGGATTAATTAATCCCCAAACCAATGGCTTGAAAGATCTTCCCCTTGGTACCAACAGGTGGTTTGAATATGTACCAAGAATGGCAGTTAGTGGGGTGAAATGGGTTGGTAATCAACTAGTGTTAGCAGGAACACTAACAATGGGTGATACAGCTACTGTACCTAGGTTAAAGTATGATCAACTAGAAAAACACTTAAACCTAGTTGCTCAAGGCCAGGGACTATTGAGAGAAGACTTGCAGATCTTCACTCCCTATGGGTGA", "aa": "MLFGGLINPQTNGLKDLPLGTNRWFEYVPRMAVSGVKWVGNQLVLAGTLTMGDTATVPRLKYDQLEKHLNLVAQGQGLLREDLQIFTPYG", "aa_hexdigest": "906032a5b7f3406e7d53e54cdf52c5f9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 36, "aa_cov": 0.3888888888888889, "hmm_cov": 1.0, "evalue": 2.7556764708305075e-10, "score": 35.104736328125, "start": 1, "stop": 35 } ], "seq_stats": { "molecular_weight": 9986.4679, "isoelectric_point": 8.085705375671388 }, "id": "KKNAAMEMLJ_433", "locus": "GCA000027325_00397" }, { "type": "cds", "sequence": "contig_1", "start": 223406, "stop": 223561, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAGTAAATTTGGTCCCCATTACTTCTTAAATAACCCTGATATCCAGGACAATGTTAATAATGATACGGTTGAAGCATTAATCAGTAGTTACAAAAACACTGATAAGTTAAAACACGTTTATCCTTATCGATACAGTGGTTTGTATGCTTGA", "aa": "MGSKFGPHYFLNNPDIQDNVNNDTVEALISSYKNTDKLKHVYPYRYSGLYA", "aa_hexdigest": "2207507d15f54f091e920decf4a08e5d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5899.470900000001, "isoelectric_point": 6.697020149230957 }, "id": "KKNAAMEMLJ_434", "locus": "GCA000027325_00398" }, { "type": "cds", "sequence": "contig_1", "start": 223646, "stop": 223789, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTGCTATCTTAAATGAAGATCTGTTAACAGGGCTAAGTGATAAGATTTTCTATGGTAAGGAGAATGAGTTTGCTGAAAATGAAGCAGATAGGTTTAACCAACTTTTAAGTTTAAATCCTAATCCTAACACTAACTGA", "aa": "MFAAILNEDLLTGLSDKIFYGKENEFAENEADRFNQLLSLNPNPNTN", "aa_hexdigest": "ec0193487adb4643e5e0fd8d51e4aa81", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5332.817600000001, "isoelectric_point": 4.1193151473999015 }, "id": "KKNAAMEMLJ_435", "locus": "GCA000027325_00399" }, { "type": "cds", "sequence": "contig_1", "start": 223855, "stop": 223992, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGGGGGGTAGAAGAGGAAGCGGAAGTTGAAGGGGAGGGGGAGTTGGAAAAGGGTTTACCATTGCCGATTCAGGGGAGAAAGTCTAAGAACTGA", "aa": "MIIDLTPTLMLEKVGRGVEEEAEVEGEGELEKGLPLPIQGRKSKN", "aa_hexdigest": "b5ffc93e38c9c068836064e565feff4a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4934.6409, "isoelectric_point": 4.605061149597168 }, "id": "KKNAAMEMLJ_436", "locus": "GCA000027325_00400" }, { "type": "cds", "sequence": "contig_1", "start": 224117, "stop": 224581, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACCTAGCAATAATAGTACCCCCTTTGATCCCAATTCAGATGATAATAAAGTCACACCATCAGGTGGCTCCTCCAAACCAACCACCTATCCTGCTTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACTAATAAGAATAACCCGCAGCGCAATCAACTGTTGCTCAGAAGCTTACTAGGAACTATTCCGGTCTTGATCAATAAGAGTGGGGATAGTAATGATCAATTTAACAAGGATAGTGAGCAGAAATGGGATAAAACTGAGACAAATGAGGGTAATTTACCTGGGTTTGGGGAGGTGAATGGGTTGTATAATGCCGCATTACTCCATACCTATGGTTTTTTTGGCACCAATACCAACTCTACTGATCCTAAGATAGGTTTTAAAGCTGATAGTAGTAGTAGTAGTAGTAGTACACTAGTAGGTAGTGGGTTAAACTGA", "aa": "MKPSNNSTPFDPNSDDNKVTPSGGSSKPTTYPALPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGDSNDQFNKDSEQKWDKTETNEGNLPGFGEVNGLYNAALLHTYGFFGTNTNSTDPKIGFKADSSSSSSSTLVGSGLN", "aa_hexdigest": "c1e353a52a9725fe272a242d146ce065", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16475.716099999983, "isoelectric_point": 5.244783973693846 }, "id": "KKNAAMEMLJ_437", "locus": "GCA000027325_00401" }, { "type": "cds", "sequence": "contig_1", "start": 224903, "stop": 225904, "strand": "+", "frame": 2, "gene": "mgpA", "product": "adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F655", "UniRef:UniRef90_UPI002244F655" ], "nt": "GTGAAGGCTTACCAAACCACTAACACCTACAACAAGTTAATTGAACCAGTTGATGCAACAAGTGCAGCAACTAACATGACCAGTTTGTTAAAACTCCTAACAACTAAAAACATCAAAGCGAAATTGGGGAAGGGAACAGCTTCTTCGCAGGGAAATAATAATGGAGGGGGTGTTAGTCAAACGATTAACACCATCACCACTACGGGAAATATTAGTGAAGGTCTAAAAGAAGAAACTAGTATTCAAGCAGAAACACTTAAAAAGTTCTTTGATAGTAAACAAAACAATAAGAGTGAAATAGGGATAGGTGATAGTACATTTACCAAGATGGATGGTAAACTAACTGGCGTAGTATCTACTCCCCTTGTTAACCTTATCAATGGCCAGGGAGCAACTAGTGATAGTGATACTGAAAAAATTAGCTTTAAACCTGGTAACCAGATTGACTTTAATAGGTTATTCACCTTACCAGTAACTGAACTATTTGATCCTAACACGATGTTTGTCTATGACCAGTATGTACCACTATTGGTTAACTTACCTAGTGGCTTTGATCAAGCTTCAATCCGCTTAAAGGTAATTAGTTACTCAGTAGAAAACCAAACCTTAGGAGTTAGATTAGAGTTCAAAGATCCTCAAACCCAACAGTTTATCCCGGTACTAAATGCATCAAGTACAGGTCCCCAAACTGTCTTTCAACCCTTTAACCAGTGGGCAGACTATGTCTTACCTTTGATTGTAACTGTTCCTATAGTAGTGATTATCCTTAGTGTTACTTTGGGATTAACGATTGGAATTCCAATGCACAGAAACAAAAAGGCATTACAAGCAGGGTTTGATCTTTCTAACAAAAAGGTTGATGTCTTGACCAAAGCAGTTGGTAGTGTCTTTAAAGAGATCATTAACAGAACAGGGATCTCTAACGCTCCTAAGAAGTTAAAACAAGCTACCCCAACCAAACCAACTCCTAAAACCCCACCAAAACCTCCAGTAAAACAATAA", "aa": "MKAYQTTNTYNKLIEPVDATSAATNMTSLLKLLTTKNIKAKLGKGTASSQGNNNGGGVSQTINTITTTGNISEGLKEETSIQAETLKKFFDSKQNNKSEIGIGDSTFTKMDGKLTGVVSTPLVNLINGQGATSDSDTEKISFKPGNQIDFNRLFTLPVTELFDPNTMFVYDQYVPLLVNLPSGFDQASIRLKVISYSVENQTLGVRLEFKDPQTQQFIPVLNASSTGPQTVFQPFNQWADYVLPLIVTVPIVVIILSVTLGLTIGIPMHRNKKALQAGFDLSNKKVDVLTKAVGSVFKEIINRTGISNAPKKLKQATPTKPTPKTPPKPPVKQ", "aa_hexdigest": "fd2213354443a4032df19153ba689ad0", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpA" ], "truncated": "5-prime", "pseudogene": { "start": 224603, "stop": 225904, "observations": { "mutation": [ 224735, 224750, 224804 ], "stop": [ 224735, 224750, 224804 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002244F655", "query_cov": 0.990990990990991, "subject_cov": 0.5481727574750831, "identity": 0.9640000000000001, "score": 582.0, "evalue": 1.12e-204, "gene_start": 4, "gene_end": 333, "reference_start": 274, "reference_end": 602, "reference_sequence": "MASLLKSVKPSNGSTSFNPTSMENQVTPSGGSSKKTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGDSNDQFNKDSEQQWNETEKPNGNLPGFGEVNGLYNAALLHTYGFFGTNTNSTDPKIGFKADSSSSSSTLVGSGLNWTSQDVGNLVVINDTSFGFQLGGWFITFTDFIRPRTGYLGITLSSLQDQTIIWADQPWTSFKGSYLDSDGTPKSLWDPTALKSLPNSSTTYDTNPTLSPSFLYFQPNKVKSGQYQQNNTYNKLIEPDNATSAATNMTNLLKLLSSKNIKQKLGKDTQSMGNNNGGGVSQTINTITTTGNISEGLKEETSIQAETLKKFFDSKQNNKSEIGIGDSTFTKMDGKLTGVVSTPLVNLINGQGATSDSDTEKISFKPGNQIDFNRLFTLPVTELFDPNTMFVYDQYVPLLVNLPSGFDQASIRLKVISYSVENQTLGVRLEFKDPQTQQFIPVLNASSTGPQTVFQPFNQWADYVLPLIVTVPIVVIILSVTLGLTIGIPMHRNKKALQAGFDLSNKKVDVLTKAVGSVFKEIINRTGISNAPKKLKQATPTKPTPKTPPKPPVKQ" }, "paralog": false, "identity": 0.9471264367816092, "score": 781.0, "evalue": 2.86e-285, "description": "Internal stop codon at 224735, 224750, 224804. Nonsense mutation around 224735, 224750, 224804.", "psc": { "uniref90_id": "UniRef90_UPI002244F655", "gene": "mgpA", "product": "adhesin P1", "uniref50_id": "UniRef50_UPI002244F655", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002244F655", "UniRef:UniRef50_UPI002244F655" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002244F655", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F655" ], "product": "adhesin P1" } }, "id": "KKNAAMEMLJ_438", "locus": "GCA000027325_00402" }, { "type": "cds", "sequence": "contig_1", "start": 225906, "stop": 226277, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAATGAGAAAACAGATTTATAAAAAAGCATACTGGTTACTATTACCCTTTCTACCATTAGCACTAGCCAATACCTTCCTTGTCAAAGAGGATAGTAAGAATGTTACTGCTTACACCCCCTTCGCCACCCCCATCACCGATTCTAAAAGTGATCTGGTTAGTTTGGCACAACTTGATTCTTCTTATCAAATCGCTGACCAAACCATCCATAACACCAACCTGTTTGTGTTGTTCAAGTCTAGGGATGTAAAAGTTAAGTATGAGTCAAGTGGCAGTAACAACATTAGTTTTGATTCAACTAGTCAAGGTGAAAAACCCTCCTATGTGGTCGAGTTTACTAACTCTACCAACATTGGCATCAAGTGA", "aa": "MKTMRKQIYKKAYWLLLPFLPLALANTFLVKEDSKNVTAYTPFATPITDSKSDLVSLAQLDSSYQIADQTIHNTNLFVLFKSRDVKVKYESSGSNNISFDSTSQGEKPSYVVEFTNSTNIGIK", "aa_hexdigest": "53ed35d3ef01858948dc0eea80e4dc8e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13854.605899999988, "isoelectric_point": 9.09843921661377 }, "id": "KKNAAMEMLJ_439", "locus": "GCA000027325_00403" }, { "type": "cds", "sequence": "contig_1", "start": 226281, "stop": 226457, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "ATGGTGAAAAAGTATCAGTTAGATGTACCGAATGTAAGTAGTGACATGAACCAAGTACTGAAAAATTTAATTCTTGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCCAAAGAGAAGGGCAAAACGCAAAGGGAGGTACATCTGGGTAGTGGGCAAGCAAATCAGTGA", "aa": "MVKKYQLDVPNVSSDMNQVLKNLILEQPLTKYTLNSSLAKEKGKTQREVHLGSGQANQ", "aa_hexdigest": "63eab8ebfae5c2760fa2eb21a3e2dec1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 226158, "stop": 226516, "observations": { "deletion": [ 226467 ], "mutation": [ 226275, 226455 ], "stop": [ 226275, 226455, 226479 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 1.0, "subject_cov": 0.4793388429752066, "identity": 0.845, "score": 97.1, "evalue": 9.01e-25, "gene_start": 1, "gene_end": 58, "reference_start": 42, "reference_end": 99, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7058823529411765, "score": 158.0, "evalue": 1.66e-51, "description": "Internal stop codon at 226275, 226455, 226479. Frameshift due to deletion around 226467. Nonsense mutation around 226275, 226455.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6514.401500000002, "isoelectric_point": 9.45623874664307 }, "id": "KKNAAMEMLJ_440", "locus": "GCA000027325_00404" }, { "type": "cds", "sequence": "contig_1", "start": 226983, "stop": 227189, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "TTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGCTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGA", "aa": "MGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQIIFSGFKAGSVGYDSSSSSSSSSSSSTKDQALA", "aa_hexdigest": "92a9eb9f04f79de9cf631132106f0c99", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 70, "aa_cov": 1.0, "hmm_cov": 0.9428571428571428, "evalue": 1.1043043700893312e-18, "score": 62.48479080200195, "start": 1, "stop": 68 } ], "seq_stats": { "molecular_weight": 7121.509300000002, "isoelectric_point": 5.974028205871583 }, "id": "KKNAAMEMLJ_441", "locus": "GCA000027325_00405" }, { "type": "cds", "sequence": "contig_1", "start": 227235, "stop": 227618, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "nt": "GTGACCAACGACACGGGGCTAAATGGTCCGATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATACAAATAATGGAACAACTGGACCCATTAAAACTGCTTATCCAGTGAAAAAAGATCAAAAATCAACTGTCAAGATCAATTCTTTGATTAACGCTACGCCCTTGAATAGTTATGGGGATGAGGGGATTGGGGTGTTTGATGCGTTAGGTTTAAACTATAACTTTAAATCTAACCAAGAACGTTTACCTTCCAGAACTGATCAGATCTTTGTTTATGGGATTGTCTCCCCTAATGAATTGCGAAGTGCTAAAAGTTCTGCTGATTCAACTGGTAGTGATACAAAGGTAAACTGA", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSGNHTNNGTTGPIKTAYPVKKDQKSTVKINSLINATPLNSYGDEGIGVFDALGLNYNFKSNQERLPSRTDQIFVYGIVSPNELRSAKSSADSTGSDTKVN", "aa_hexdigest": "68ef5d111c384ae5f6513ea65311544c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F305329", "query_cov": 0.8818897637795275, "subject_cov": 0.9824561403508771, "identity": 0.589, "score": 138.0, "evalue": 4.35e-40, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_A0AB38W8G0" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "KKNAAMEMLJ_442", "locus": "GCA000027325_00406" }, { "type": "cds", "sequence": "contig_1", "start": 227823, "stop": 229067, "strand": "+", "frame": 3, "gene": null, "product": "Mgp-operon protein 3", "db_xrefs": [ "GO:0005886", "GO:0007155", "SO:0001217", "UniRef:UniRef50_P22747", "UniRef:UniRef90_P22747" ], "nt": "ATGAGTGGTAAGAAAGCTAACTATGGAGCGGTTGTGTTAACACGGGGGGGTGTTGTTAGATTAAACTTTAACCCTGGTAATGATTCATTGCTTTCAACAACTGATAACAATATAGCACCTATCTCCTTCTCATTTACTCCGTTCACAGCTGCTGAGAGTGCGGTGGATCTCACTACCTTCAAAGAAGTTACCTATAACCAAGAATCAGGGTTATGGAGTTATATCTTTGACAGCTCCTTAAAACCAAGCCATGATGGTAAACAAACTCCTGTCACTGATAACATGGGCTTTAGTGTTATCACTGTCTCAAGAACTGGCATTGAACTAAACCAAGACCAAGCTACTACAACTCTTGATGTAGCACCTAGTGCACTAGCAGTGCAATCAGGGATCCAATCTACCACCCAAACCCTAACTGGAGTACTCCCACTTAGTGAGGAATTCAGTGCAGTTATTGCTAAAGATAGTGATCAAAATAAGATTGATATCTATAAAAACAACAACGGGTTGTTTGAAATTGATACCCAACTAAGTAATAGTGTTGCCACCAACAACGGTGGGTTAGCACCTAGTTACACAGAAAACAGGGTTGATGCATGGGGTAAAGTTGAGTTTGCTGATAACAGTGTATTGCAAGCAAGAAACCTAGTTGATAAAACTGTTGATGAGATCATCAATACCCCTGAAATCTTAAACTCCTTCTTTAGATTCACCCCTGCTTTTGAAGATCAAAAAGCTACCCTTGTTGCTACTAAGCAAAGTGATACATCACTTAGTGTCTCACCAAGGATCCAGTTCTTAGATGGTAATTTCTATGATCTTAACTCTACCATCGCTGGGGTACCTTTAAACATTGGTTTCCCTTCAAGAGTGTTTGCTGGGTTTGCAGCACTCCCTGCATGGGTGATCCCTGTATCAGTAGGTTCTTCAGTTGGGATCTTGTTTATCTTGTTAGTCTTAGGACTTGGGATTGGGATCCCAATGTACAGGGTAAGAAAACTCCAAGATGCATCGTTTGTTAATGTCTTTAAAAAGGTTGATACACTCACAACTGCTGTCGGTAGTGTGTACAAAAAGATTATTACCCAAACTGGTGTGGTGAAAAAAGCACCTAGTGCATTGAAAGCTGCTAATCCTAGTGTTAAAAAACCTGCTGCTTTTTTAAAACCACCTGTTCAACCACCAAGTAAACCTGAAGGGGAACAAAAAGCTGTTGAAGTTAAGTCAGAAGAAACCAAAAGTTAG", "aa": "MSGKKANYGAVVLTRGGVVRLNFNPGNDSLLSTTDNNIAPISFSFTPFTAAESAVDLTTFKEVTYNQESGLWSYIFDSSLKPSHDGKQTPVTDNMGFSVITVSRTGIELNQDQATTTLDVAPSALAVQSGIQSTTQTLTGVLPLSEEFSAVIAKDSDQNKIDIYKNNNGLFEIDTQLSNSVATNNGGLAPSYTENRVDAWGKVEFADNSVLQARNLVDKTVDEIINTPEILNSFFRFTPAFEDQKATLVATKQSDTSLSVSPRIQFLDGNFYDLNSTIAGVPLNIGFPSRVFAGFAALPAWVIPVSVGSSVGILFILLVLGLGIGIPMYRVRKLQDASFVNVFKKVDTLTTAVGSVYKKIITQTGVVKKAPSALKAANPSVKKPAAFLKPPVQPPSKPEGEQKAVEVKSEETKS", "aa_hexdigest": "1518b9af823f8ee5544eafe97dd6744a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 227523, "stop": 229067, "observations": { "mutation": [ 227616 ], "stop": [ 227616 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P22747", "query_cov": 1.0289855072463767, "subject_cov": 0.4018867924528302, "identity": 0.955, "score": 750.0, "evalue": 7.77e-263, "gene_start": 1, "gene_end": 414, "reference_start": 635, "reference_end": 1060, "reference_sequence": "MKTMRKQIYKKAYWLLLPFLPLALANTFLVKEDSKNVTAYTPFATPITDSKSDLVSLAQLDSSYIISDQTIHNTNLFVLFKSTQVKLTYNSSSGSNNQISFDSTNNKPSYIVEFTNSTNIGIKWRMVKKYQLDVPNVSSNMNDVLKELILEQPLTKYTLNSSLAKEKGKTQREVHLGSGQATRWTSQRNQHDLNNNPSPNASTGFKLTTGNAYRKLDQSWPIYQPIDGLKDGKGKDQANWQSSEQSTAASDAPLSTGGGSSSGSFNKYLNTKQALESIGILFDDQTPRNVITQLYYASTSKLAVTNNHIVVMGNSFLPSLWYWVVERSAQENASNKPTWFANTNLDWGEDKQKQFVENQLGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQLIFSGFKAGSVGYDSSSSSSSTKDQALAWSTTTSLDSKTGYKDLVTNDTGLNGPINGSFSIQDTFSFVVPYSGNHTNNGTTGTIKTAYPVKNTEKATVKINSLINATPLNSYGDEGIGVFDALGLNYNFKSNQERLPSRTDQIFVYGIVSPNELRSAKSSADSTSSDTKVNWTNTQSRYLPVPYNYSEGIIDADGFKRPENRGANVTIFSGLKSIAPDGFANSIANFSVGLKAGIDPNPVMSGKKANYGAVVLTRGGVVRLNFDPGNDSLLSTTDNNIAPISFSFTPFTAAESAVDLTTFKEVTYNQESGLWSYIFDNSLKPTHDGKQTPVTDNKGFSVITVSRTGIELNQDQATTTPLDVAPSALAVQSGIQSTTQTLTGVLPLSEEFSAVIAKNSDQNKIDIYKNNNGLFEIDTQLSNSVATNNGGLAPSYTENEVDAWGKVEFADNSVLQARNLVDKTVDEIINTPEILNSFFRFTPAFEDQKATLVATKQSDTSLSVSPRIQFLDGNFYDLNSTIAGVPLNIGFPSRVFAGFAALPAWVIPVSVGSSVGILFILLVLGLGIGIPMYRVRKLQDASFVNVFKKVDTLTTAVGSVYKKIITQTGVVKKAPSALKAANPSVKKPAAFLKPPVQPAKPAAKPPVQPPSKPEGEQKAVEVKSEETKS" }, "paralog": false, "identity": 0.9543726235741445, "score": 966.0, "evalue": 0.0, "description": "Internal stop codon at 227616. Nonsense mutation around 227616.", "psc": { "uniref90_id": "UniRef90_P22747", "product": "Mgp-operon protein 3", "uniref50_id": "UniRef50_P22747", "go_ids": [ "GO:0005886", "GO:0007155" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P22747", "UniRef:UniRef50_P22747", "GO:0005886", "GO:0007155" ] }, "pscc": { "uniref50_id": "UniRef50_P22747", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P22747" ], "product": "Mgp-operon protein 3" } }, "id": "KKNAAMEMLJ_443", "locus": "GCA000027325_00407" }, { "type": "cds", "sequence": "contig_1", "start": 229461, "stop": 229811, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTAAGGTGGTATTAGAGTACCACAAGCTCAGTAAGGATGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCTTTTGATCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATTCAAGTAAACAAGGGGAGAAACTCAGTGAAACAACTGCTTCATCCATGAGTGGTATGGCTACATCTCCTCGCAAGGCCCTTAAGATAGAGGTGGAGAAAGGTTCTTCAGGGTCTGACACCCTCACCAAATCCGACTTTGCTAAAAAGCCACTGAAACATAAAGAAAATAGTGGGACAGAGGTGAAGTTGGATGCACAGAAGGATTTTGCCGGAGAGAAGGCCTGA", "aa": "MPKVVLEYHKLSKDVVKESLEVEATDSFDPTQRLQKDSPMKDSSKQGEKLSETTASSMSGMATSPRKALKIEVEKGSSGSDTLTKSDFAKKPLKHKENSGTEVKLDAQKDFAGEKA", "aa_hexdigest": "54a3f09954959c9b1790b96b6bc6b153", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D24C90", "query_cov": 0.9137931034482759, "subject_cov": 0.8346456692913385, "identity": 0.8109999999999999, "score": 150.0, "evalue": 5.7e-45, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12676.145599999983, "isoelectric_point": 7.873991203308106 }, "id": "KKNAAMEMLJ_444", "locus": "GCA000027325_00408" }, { "type": "cds", "sequence": "contig_1", "start": 230768, "stop": 230944, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49391", "UniRef:UniRef90_UPI002FCE0920" ], "nt": "ATGGTGAAAAAGTATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACGATGTACTGAAAAATTTAATTCTTGAACAACCCCTTACCAAGTATACGCTTAATAGTAGTTTGGCTAAAGAGAAGGGTAAGACACAAGTAGCGGTACATCTGGGTAGTGGGCAAGCAACTAATTGA", "aa": "MVKKYQLDVPNVSSNMNDVLKNLILEQPLTKYTLNSSLAKEKGKTQVAVHLGSGQATN", "aa_hexdigest": "967e7765efdd3b7a0a7ebb25917e5e3c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 230630, "stop": 230971, "observations": { "mutation": [ 230762, 230942 ], "stop": [ 230762, 230942 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE0920", "query_cov": 1.0, "subject_cov": 0.5043478260869565, "identity": 0.862, "score": 96.3, "evalue": 1.54e-24, "gene_start": 1, "gene_end": 58, "reference_start": 50, "reference_end": 106, "reference_sequence": "MLFKSRDVKVKYESSGSNNISFENSNSQNNKPSYVVEFTNSTNVGIKWTMVKKYQLDVPNVSSDMNQVLQDLILEQPLTKYTLNGSLAKEKGKSQVAVHLGGQANNWQSMRHSMA" }, "paralog": false, "identity": 0.7931034482758621, "score": 172.0, "evalue": 2.5e-57, "description": "Internal stop codon at 230762, 230942. Nonsense mutation around 230762, 230942.", "psc": { "uniref90_id": "UniRef90_UPI002FCE0920", "uniref50_id": "UniRef50_Q49391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE0920", "UniRef:UniRef50_Q49391" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6358.258800000003, "isoelectric_point": 9.457012367248538 }, "id": "KKNAAMEMLJ_445", "locus": "GCA000027325_00409" }, { "type": "cds", "sequence": "contig_1", "start": 232282, "stop": 232842, "strand": "+", "frame": 1, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_P75564", "UniRef:UniRef90_P47436" ], "nt": "ATGCTACCAGCAACAGAGTTTACCAATGGTTCTAATAACGCACTATATCAGGTTATTGATAATTTAGTTGAATACTTTAAAAGCTTTTTATTCACAATTAATTTTGATAGTGAACTGACCAGTATTAGTGACTGTTTTGATCTTTTAAATATCCCTAAAGATCATTCCAGTAGGAATGAATCTGATTCTTTTTATATCGATAAAACCAGTTTATTGAGAACCCATTGTACTGCTACCACGCTAAAAGCAGTCAGAACTTCTAAAAAAACTAATAATCCTGATATCAGGGTTGTCTCTTTAGGAGCGGTTTTTCGTAATGATAGTGATGATGCCACCCACTCCCATCAGTTTACCCAACTTGATTTTATGTGGATTAAAAAAGGGCTTTCATTAGCTAATTTAAAGTGGTTTATTAACAATATGATCACCCATTTCTTTGGGGAAAATACTTTTACTAGGTTTAGACTATCCCACTTCCCATTCACTGAACCCTCGTTTGAAATTGACATTAGGTGTTGGTTATGTCAAAATGGTTGTTCTATTTGTAAGCAAACCAAGTGA", "aa": "MLPATEFTNGSNNALYQVIDNLVEYFKSFLFTINFDSELTSISDCFDLLNIPKDHSSRNESDSFYIDKTSLLRTHCTATTLKAVRTSKKTNNPDIRVVSLGAVFRNDSDDATHSHQFTQLDFMWIKKGLSLANLKWFINNMITHFFGENTFTRFRLSHFPFTEPSFEIDIRCWLCQNGCSICKQTK", "aa_hexdigest": "bf61341790ccddf0dd3cf8ca71deaf9c", "start_type": "ATG", "rbs_motif": null, "genes": [ "pheS" ], "truncated": "both", "pseudogene": { "start": 232009, "stop": 233034, "observations": { "mutation": [ 232039, 232840 ], "stop": [ 232039, 232840 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47436", "query_cov": 1.0, "subject_cov": 0.5454545454545454, "identity": 1.0, "score": 377.0, "evalue": 2.77e-130, "gene_start": 1, "gene_end": 186, "reference_start": 92, "reference_end": 277, "reference_sequence": "MIDQNKLITKWKKAFAKAKNLTTLVNLKNTLHNSDLKPLLQKIKTATKLSEKSSLGKLYQSLDIQLTDLLTSYKKTFEINNQVSQKPSLDVMLPATEFTNGSNNALYQVIDNLVEYFKSFLFTINFDSELTSISDCFDLLNIPKDHSSRNESDSFYIDKTSLLRTHCTATTLKAVRTSKKTNNPDIRVVSLGAVFRNDSDDATHSHQFTQLDFMWIKKGLSLANLKWFINNMITHFFGENTFTRFRLSHFPFTEPSFEIDIRCWLCQNGCSICKQTKWIEILGAGIIHPQVMNNMGIGDTENITGIAAGIGIERLAMLKYGIDDIRDFYDNNFKFLTQFTD" }, "paralog": false, "identity": 0.9941348973607038, "score": 681.0, "evalue": 2.13e-252, "description": "Internal stop codon at 232039, 232840. Nonsense mutation around 232039, 232840.", "psc": { "uniref90_id": "UniRef90_P47436", "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_P75564", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47436", "EC:6.1.1.20", "UniRef:UniRef50_P75564", "COG:COG0016", "COG:J", "KEGG:K01889", "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_P75564", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75564" ], "product": "Phenylalanine--tRNA ligase alpha subunit" } }, "id": "KKNAAMEMLJ_446", "locus": "GCA000027325_00410" }, { "type": "cds", "sequence": "contig_1", "start": 232882, "stop": 233034, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGAATAACATGGGAATTGGGGATACTGAAAATATTACTGGGATAGCAGCAGGAATTGGGATTGAACGCTTAGCAATGTTAAAGTATGGGATTGATGATATCCGTGATTTTTATGATAACAACTTTAAGTTTTTAACCCAGTTTACTGACTAA", "aa": "MNNMGIGDTENITGIAAGIGIERLAMLKYGIDDIRDFYDNNFKFLTQFTD", "aa_hexdigest": "c10ad6a6cdb630eab0435e14e5630067", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 47, "aa_cov": 0.94, "hmm_cov": 0.9574468085106383, "evalue": 6.807337216233565e-16, "score": 53.52961349487305, "start": 2, "stop": 48 } ], "seq_stats": { "molecular_weight": 5634.288600000001, "isoelectric_point": 4.293526649475097 }, "id": "KKNAAMEMLJ_447", "locus": "GCA000027325_00411" }, { "type": "cds", "sequence": "contig_1", "start": 233038, "stop": 233511, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTGATATCAAAAAAAACACTTGGCGTTTTAATCCCTGACATCTTTAGTTTTTCTAATGATCAAATTGCCCAAAAGTTAGAACAAATGGGGATTGAAGTGGAATCAATTAAGCAGTTTAACAGCCCTGATTACCTCCAACTTGCAAAGGTTGTATCAATCCAACCCCATCCCCATGACAACAAGCTTTTTATCTGTGAATTACAAATTGATAAAAACAAGTTTATTAATGTTGTTTCCAATGCTAATAACATTAACAATCCTGATAATATCAACAAGTTTGTCATTGTTGCAAAAAAAGGAACTGAGTTACTCAACGGGTTAATTGTTAAAACCCAAAATATTAAAGGGATCATTTCAGAAGGGATTTTATGTAGCTATATTGACATTAACCCCTTCAGTAGACAGATCATTGAAAAAACAGAAGTTGCTGATGCGATTATCATTGATCATGTTAGCAATGATCATGACTGA", "aa": "MLISKKTLGVLIPDIFSFSNDQIAQKLEQMGIEVESIKQFNSPDYLQLAKVVSIQPHPHDNKLFICELQIDKNKFINVVSNANNINNPDNINKFVIVAKKGTELLNGLIVKTQNIKGIISEGILCSYIDINPFSRQIIEKTEVADAIIIDHVSNDHD", "aa_hexdigest": "0376e536e714d56cc3e409b342b40556", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 94, "aa_cov": 0.5987261146496815, "hmm_cov": 0.9148936170212766, "evalue": 8.435154538943523e-14, "score": 46.59867858886719, "start": 47, "stop": 140 } ], "seq_stats": { "molecular_weight": 17611.09349999999, "isoelectric_point": 5.525227546691894 }, "id": "KKNAAMEMLJ_448", "locus": "GCA000027325_00412" }, { "type": "cds", "sequence": "contig_1", "start": 233545, "stop": 233772, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTTGATGTTAAAACCCCAACTAACAGAGCAGATCTTCATAGCTTAATCTTTTTAGCAAAAGAACTTGGGGTACTTTTGAAAACCAAAACCTTTTTAAAACAAAAAAGTAGTGTTGTTAACCATGACTTTTTTAAGTTTCCCCTAAATTTAAAAAACAAGTTAAAAGCGAATTATTTTGGCGGTTTGTTCTTAAGACAAATTAACCAACATAGTTCACCTTGA", "aa": "MIFDVKTPTNRADLHSLIFLAKELGVLLKTKTFLKQKSSVVNHDFFKFPLNLKNKLKANYFGGLFLRQINQHSSP", "aa_hexdigest": "c256bba9dff5f863cb54becac8b7c18a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8648.089899999997, "isoelectric_point": 10.237402153015136 }, "id": "KKNAAMEMLJ_449", "locus": "GCA000027325_00413" }, { "type": "cds", "sequence": "contig_1", "start": 233800, "stop": 234444, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03483.22" ], "nt": "ATGATCAAACCAGTTAACTATTATGTTGATAAAGCTAACTTAGTAACAGTGTTCACCGCTCAGCCAATCCATTGTCATGATGCAGATAGAATTGTTGGTAACATTGAACTTAAACAAGCAACCCATAATGAAACTTTTGTTGGACTTGATGACAAGCAATATGAGATTGAACCAGGGGATATTGTTGTTTGTGATGAGAAGGGCATTATTGCACTGGTAGGGATCATTGGTTCAAAGCGCACAATGGTCCAACCTACAACAACTAACATCTTTTTTGAAGTTGTTAACTGTAACAGTGAAACCATTAAACAAACTGCCAAGCGCTTTTTGATCAATAACTTTGCCAGTAAGTTTATGGTTAAACCGATTAGCTTATTAGCTACTGATAACTGTTTAAACTACTTACAAAACAGTTTACTAACCACTGATAACATTGGCAAAATTAGCCACTTTTCAAGTTCGCTTAAAGTTGAACCATTTAGTAAAAAGCTCACAGTGAATTTCCATAAGATACGCCAACTAATTGGCATTGAAAAAAAGGAACTAACTGATCAAACCATTAAAAAAAGCCTCAGTCAACTAGGGTTTAAAGTTGACAACCAACTTCTCAAAATCCCCAGTTACAGACAAGACATTAATACCTGA", "aa": "MIKPVNYYVDKANLVTVFTAQPIHCHDADRIVGNIELKQATHNETFVGLDDKQYEIEPGDIVVCDEKGIIALVGIIGSKRTMVQPTTTNIFFEVVNCNSETIKQTAKRFLINNFASKFMVKPISLLATDNCLNYLQNSLLTTDNIGKISHFSSSLKVEPFSKKLTVNFHKIRQLIGIEKKELTDQTIKKSLSQLGFKVDNQLLKIPSYRQDINT", "aa_hexdigest": "2e3fc53a1a13632e33e2a11b1dc81f15", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03483.22", "name": "B3_4", "length": 140, "aa_cov": 0.6542056074766355, "hmm_cov": 1.0, "evalue": 3.040482865783297e-41, "score": 135.9696044921875, "start": 2, "stop": 141 } ], "seq_stats": { "molecular_weight": 24162.667700000024, "isoelectric_point": 8.787379264831543 }, "id": "KKNAAMEMLJ_450", "locus": "GCA000027325_00414" }, { "type": "cds", "sequence": "contig_1", "start": 234466, "stop": 234849, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "GTGAAGTTAATTGATATCAATAAGTTAAAACCAATTGGGATCACTAGTAGTTTTAACTTTGAAAAGTCCAGTTACTTTAACACTTTTAATGCTTTAACAAAACTAAGAAAAAAGCTACAAACACTTGGTTTTCACAACGTTATTACCTACCAGTTAACTGATCAAAAAAGTGCAAAAACTTTTAATTTGTTTAACTTAGAAAATTTCATCACCATTAAAAACCCAGTGTCCCAAAACCATTCTGTAATGCGTGTTAGCTTAATTGATTCACTGTTAAAAGTGCTAAAAACCAATAACAACTATAAGAATGAACTGGTGAACATCTTTGAGTTTTCCTTTATTAAAACCCAAAACAATAGTGAACTGCACCTGGCAGTATTATGA", "aa": "MKLIDINKLKPIGITSSFNFEKSSYFNTFNALTKLRKKLQTLGFHNVITYQLTDQKSAKTFNLFNLENFITIKNPVSQNHSVMRVSLIDSLLKVLKTNNNYKNELVNIFEFSFIKTQNNSELHLAVL", "aa_hexdigest": "347863b2eed081ca10fe8d16db906b17", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 90, "aa_cov": 0.7007874015748031, "hmm_cov": 1.0, "evalue": 2.5948311122068317e-21, "score": 71.14582061767578, "start": 30, "stop": 118 } ], "seq_stats": { "molecular_weight": 14717.890799999988, "isoelectric_point": 9.84382266998291 }, "id": "KKNAAMEMLJ_451", "locus": "GCA000027325_00415" }, { "type": "cds", "sequence": "contig_1", "start": 234883, "stop": 235284, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "ATGCAAGGGATAAGCAATGATGTTTTTACTATGAAGGGATTAGCAAAACTCATTGTTGCTAACTTAGGGTTTAGTTGTGACTTTGAACCACTTGATGATAGTGACTATTTTGTTAATAATCAAAGTTTAAAAATAGTAGTTTTTAACGAACAGATCGGTTTTATTGGGCTAATTAAAGAATCATTGTTAAATAACTATGATCTGAACAATAAACCCATTTATTGTCTTGAAATCAACTTAGATAGGATGCTCTCTTCTCTAAACAGGATTGAAAAAAACTACCTTGGTTACAGTAAACTACAACCTGTTTGCAAGGATCTTACCTTTAGTTTTACCAACCCTGCTAGTCACTTTGATCAGTTTGCTAACATGATCAAAAGGATAACTGGCATTGAAAGTTGA", "aa": "MQGISNDVFTMKGLAKLIVANLGFSCDFEPLDDSDYFVNNQSLKIVVFNEQIGFIGLIKESLLNNYDLNNKPIYCLEINLDRMLSSLNRIEKNYLGYSKLQPVCKDLTFSFTNPASHFDQFANMIKRITGIES", "aa_hexdigest": "3b6cebd8dfbbba80434279576b7aaff3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 76, "aa_cov": 0.5639097744360902, "hmm_cov": 0.9736842105263158, "evalue": 1.0559634116442835e-13, "score": 46.27659606933594, "start": 6, "stop": 80 } ], "seq_stats": { "molecular_weight": 15145.228299999984, "isoelectric_point": 5.035218620300292 }, "id": "KKNAAMEMLJ_452", "locus": "GCA000027325_00416" }, { "type": "cds", "sequence": "contig_1", "start": 235309, "stop": 235458, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACAACCAACTGATCACTAAGTACACCGTTCGTTATTTTCTGAAAAATGATGCTAACAAACCACTAACTAACCAAACAATTGAACTTATCACTAATAACTTAAAACTCCAGTGTGAAAAACTAAAAATTAAATTAGATATTTAG", "aa": "MQNNQLITKYTVRYFLKNDANKPLTNQTIELITNNLKLQCEKLKIKLDI", "aa_hexdigest": "758432b39fa1512bcb857ab864ede4d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5822.796399999998, "isoelectric_point": 9.51832180023193 }, "id": "KKNAAMEMLJ_453", "locus": "GCA000027325_00417" }, { "type": "cds", "sequence": "contig_1", "start": 235517, "stop": 236062, "strand": "+", "frame": 2, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0032790", "GO:0043022", "RefSeq:WP_009885737.1", "SO:0001217", "UniParc:UPI0000165F7E", "UniRef:UniRef100_UPI0000165F7E", "UniRef:UniRef50_P47438", "UniRef:UniRef90_P47438" ], "nt": "TTGGCACAAAACGCAAAACACCCTTCTAAAAAAGAACAAAAACCACTCGTTAATGAACAGATTGCTTTCAATCAGTTCACTTTAATTGATGAAAACAGTACTAATTTAGGGATAGTTAAGATGGAAAACGCTTTAAAGTTAGCACAAGAAAAACAGTTAGATCTAGTTCTAATTGCTCCAAACCCAACCAAACCGATCGTTAAGTTGTTGGACTTTGGCAGATATACCTATGATTTAAAGCGTAAGAAAAGACAAGCCAAGAAAAACCAAACAATCATCCAAACCAAAGAAGTTGTTGTCAAACCAACGATTGCTAAACATGATTTAGAATTTAGAGCAAAACAGAGTAAGAATTGGATAGAAAAAGGTCATCATGTCAAGTTTATAGTCCGTGCCTTTGGCAGGGTTAGCACCAGGATAGAGTTAATTGAAAAGGTGTTTGATGACTTTTACCAGTTAGTTAAAGATGTAGTTGAGATCCAAAAACCTTTAACCGCTTCTTCCAAAACGATGTACGCTGCTCTATTAGTACCTTTAAAAAGATAG", "aa": "MAQNAKHPSKKEQKPLVNEQIAFNQFTLIDENSTNLGIVKMENALKLAQEKQLDLVLIAPNPTKPIVKLLDFGRYTYDLKRKKRQAKKNQTIIQTKEVVVKPTIAKHDLEFRAKQSKNWIEKGHHVKFIVRAFGRVSTRIELIEKVFDDFYQLVKDVVEIQKPLTASSKTMYAALLVPLKR", "aa_hexdigest": "10d5dec888ea3d6eadcff8182f502af4", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F7E", "ncbi_nrp_id": "WP_009885737.1", "uniref100_id": "UniRef100_UPI0000165F7E", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F7E", "RefSeq:WP_009885737.1", "UniRef:UniRef100_UPI0000165F7E" ] }, "ips": { "uniref100_id": "UniRef100_UPI0000165F7E", "uniref90_id": "UniRef90_P47438" }, "psc": { "uniref90_id": "UniRef90_P47438", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_P47438", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P47438", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47438" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "KKNAAMEMLJ_454", "locus": "GCA000027325_00418" }, { "type": "cds", "sequence": "contig_1", "start": 236065, "stop": 236244, "strand": "+", "frame": 1, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "KEGG:K02916", "RefSeq:WP_009885738.1", "SO:0001217", "UniParc:UPI0000133FF7", "UniRef:UniRef100_P47439", "UniRef:UniRef50_P47439", "UniRef:UniRef90_P47439" ], "nt": "ATGAAAACCAAAAGTGCTGCAGTAAAACGCTTTAAACTCACCAAATCAGGACAAATTAAGCGCAAACACGCTTATACTTCCCACCTCGCGCCCCACAAATCAACCAAACAAAAGCGCCATTTGCGCAAGCAAGCTACTGTGAGCAACAGTGAATTGAAAAGAATTGGTATTTTAATTTAG", "aa": "MKTKSAAVKRFKLTKSGQIKRKHAYTSHLAPHKSTKQKRHLRKQATVSNSELKRIGILI", "aa_hexdigest": "a92c22b563615ebee0f1d0ca7619ae8f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FF7", "ncbi_nrp_id": "WP_009885738.1", "uniref100_id": "UniRef100_P47439", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FF7", "RefSeq:WP_009885738.1", "UniRef:UniRef100_P47439" ] }, "ips": { "uniref100_id": "UniRef100_P47439", "uniref90_id": "UniRef90_P47439" }, "psc": { "uniref90_id": "UniRef90_P47439", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P47439", "cog_id": "COG0291", "cog_category": "J", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47439", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47439" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "KKNAAMEMLJ_455", "locus": "GCA000027325_00419" }, { "type": "cds", "sequence": "contig_1", "start": 236247, "stop": 236621, "strand": "+", "frame": 3, "gene": "rplT", "product": "50S ribosomal protein L20", "db_xrefs": [ "COG:COG0292", "COG:J", "GO:0000027", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625", "RefSeq:WP_009885739.1", "SO:0001217", "UniParc:UPI0000133D17", "UniRef:UniRef100_P47440", "UniRef:UniRef50_Q7NBC0", "UniRef:UniRef90_P47440" ], "nt": "ATGCGTGTTAAGGGAACAAATACAACCAGGATTAGAAGAAAAAAATGGTTAAAACAAGCTAGTGGTAGCTTTGGGACAAGAAAAGCTTCTTTTAAGGCAGCTAAACAAACTGTTATCCAAGCAAGCAAGTATGCTTACCGTGATAGGAGACAGAAAAAACGTGAGTTTCGTTCGTTGTGGATCTTAAGGTTAAATGCTGCACTGCGTGCACAAGGGATGACTTATTCAGTGTTTATCAATGAATTGAAAAAAGCCAAGATAGTCATTAACAGAAAGGTACTTTCTGAACTAGCAATTAAAGAACCTAATAAGTTAAATCTGATTATCAATACCATCAAAAAACCAACTAATAAACCAACTGTTGCAAAAACTTAG", "aa": "MRVKGTNTTRIRRKKWLKQASGSFGTRKASFKAAKQTVIQASKYAYRDRRQKKREFRSLWILRLNAALRAQGMTYSVFINELKKAKIVINRKVLSELAIKEPNKLNLIINTIKKPTNKPTVAKT", "aa_hexdigest": "7434c48886aa79e5163f3a2be45cec80", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D17", "ncbi_nrp_id": "WP_009885739.1", "uniref100_id": "UniRef100_P47440", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D17", "RefSeq:WP_009885739.1", "UniRef:UniRef100_P47440" ] }, "ips": { "uniref100_id": "UniRef100_P47440", "uniref90_id": "UniRef90_P47440" }, "psc": { "uniref90_id": "UniRef90_P47440", "gene": "rplT", "product": "50S ribosomal protein L20", "uniref50_id": "UniRef50_Q7NBC0", "cog_id": "COG0292", "cog_category": "J", "go_ids": [ "GO:0000027", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q7NBC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7NBC0" ], "product": "Large ribosomal subunit protein bL20" }, "genes": [ "rplT" ], "id": "KKNAAMEMLJ_456", "locus": "GCA000027325_00420" }, { "type": "cds", "sequence": "contig_1", "start": 236593, "stop": 237300, "strand": "-", "frame": 3, "gene": "rnhC", "product": "Ribonuclease HIII", "db_xrefs": [ "COG:COG1039", "COG:L", "EC:3.1.26.4", "GO:0003723", "GO:0004523", "GO:0005737", "GO:0006298", "GO:0032299", "GO:0043137", "GO:0046872", "RefSeq:WP_009885740.1", "SO:0001217", "UniParc:UPI0000557D0F", "UniRef:UniRef100_P47441", "UniRef:UniRef50_P47441", "UniRef:UniRef90_P47441" ], "nt": "TTGCAACATTACAAACCAGCTGATTTTTATTTAATTGGCAGTGATGAATCTGGCAAGGGTGATAGTTTTGGTGGGATTTGTGTTAGTGCTGTTTTAATTGAAAAAAAACAGCTTATTAATCTTGAAAATTTACAAGTAACTGATTCAAAAAAACTCAGTGATCACACTGTCAAACTGTTAGCTAAATCGATCCAAACAACAGTAATGGATCACCATACAATTACATTAGATCCCAAGCAGTATAATGATTTGACAAAATCACTGAAAAATACCAATTTACTTTTGACCAATTTACACTGTCAGCTTTACCAAAAGTTACTGGAAAAAAATCAGTTATTAAGACAAACAGTAACAATTAGCATTGACCAGTTTGCCAATCAAGAGTTATTTGTTAACTATCTAAATAAGCTAACTAATTTCACTGATAAAACAGTGTTATTACCTGATCAGTTTCTAATTAATGGTGAAACTAAGTCACTGGTGATTGCAGCTGCATCTATCTTAGCAAGAGATAGCTTTATTAATCAGCTGCAATTACTCAACCAAAAGGTAAACTATGATTTACCCAAAGGCAGTAGTCATGGCATTGAACAAGCGTTGTTATTTCTTAACCAACAACGCGGTTTTAGTCAAATTGAACAGTTTAAACAGGTGGCAAAACTTAACTTTAAAAACGTTACTAAGTTTTTGCAACAGTTGGTTTATTAG", "aa": "MQHYKPADFYLIGSDESGKGDSFGGICVSAVLIEKKQLINLENLQVTDSKKLSDHTVKLLAKSIQTTVMDHHTITLDPKQYNDLTKSLKNTNLLLTNLHCQLYQKLLEKNQLLRQTVTISIDQFANQELFVNYLNKLTNFTDKTVLLPDQFLINGETKSLVIAAASILARDSFINQLQLLNQKVNYDLPKGSSHGIEQALLFLNQQRGFSQIEQFKQVAKLNFKNVTKFLQQLVY", "aa_hexdigest": "c2dc8a4dbeb8be236918658f2814e7a0", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557D0F", "ncbi_nrp_id": "WP_009885740.1", "uniref100_id": "UniRef100_P47441", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557D0F", "RefSeq:WP_009885740.1", "UniRef:UniRef100_P47441" ] }, "ips": { "uniref100_id": "UniRef100_P47441", "uniref90_id": "UniRef90_P47441" }, "psc": { "uniref90_id": "UniRef90_P47441", "gene": "rnhC", "product": "Ribonuclease HIII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_P47441", "cog_id": "COG1039", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0032299", "GO:0046872", "GO:0003723", "GO:0004523", "GO:0043137", "GO:0006298" ] }, "pscc": { "uniref50_id": "UniRef50_P47441", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47441" ], "product": "Ribonuclease HIII" }, "genes": [ "rnhC" ], "id": "KKNAAMEMLJ_457", "locus": "GCA000027325_00421" }, { "type": "cds", "sequence": "contig_1", "start": 237347, "stop": 237913, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00226.36", "PFAM:PF16713.10" ], "nt": "ATGGCTGAACAGAAACGTGATTATTATGAAGTGTTAGGAATAACCCCTGATGCTGATCAATCAGAGATTAAAAAAGCCTTTCGTAAGCTGGCTAAAAAGTACCATCCTGATCGTAACAACGCGCCTGATGCTGCTAAGATTTTTGCTGAAATTAATGAAGCAAATGATGTTTTATCAAACCCCAAAAAAAGAGCTAACTATGATAAGTATGGTTTTGATGGGGTTGATGGTGAACCTGCTTTTAACTTCCAAGCAGATGTTTTTCAATCCTTTTTTGAAGAGATAGCAAAATCAGGGGTTTTTAACAACCAAACCAATCCTGAACAAAAAGAAAAAAAGAAACGTTACCACTGGTTCTCCAAAAAACCTAAGCAAGAACAACCTGAAATTAACTTAGATCACGTTGTTGAGCAAACCATTAAAAAGGTGCAACAAAACCAAAACCAAAACAAAGACCCAGATGAATTACGTTCTAAGGTCCCTGGAGAGGTTACTGCTAGTGATTGGGAAGCATTGGTTGGTGATACTAGGTATGGGTATTTTGATGAAACAGGGGATTGGAGTTGA", "aa": "MAEQKRDYYEVLGITPDADQSEIKKAFRKLAKKYHPDRNNAPDAAKIFAEINEANDVLSNPKKRANYDKYGFDGVDGEPAFNFQADVFQSFFEEIAKSGVFNNQTNPEQKEKKKRYHWFSKKPKQEQPEINLDHVVEQTIKKVQQNQNQNKDPDELRSKVPGEVTASDWEALVGDTRYGYFDETGDWS", "aa_hexdigest": "8eec91dfc27ac89293ba85081ca7f992", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00226.36", "name": "DnaJ", "length": 63, "aa_cov": 0.32978723404255317, "hmm_cov": 1.0, "evalue": 3.6988033143826847e-28, "score": 92.57559204101562, "start": 7, "stop": 68 }, { "id": "PF16713.10", "name": "EAGR_box", "length": 20, "aa_cov": 0.10638297872340426, "hmm_cov": 1.0, "evalue": 3.435657692549014e-08, "score": 28.173444747924805, "start": 168, "stop": 187 } ], "seq_stats": { "molecular_weight": 21786.700399999994, "isoelectric_point": 5.370170783996582 }, "id": "KKNAAMEMLJ_458", "locus": "GCA000027325_00422" }, { "type": "cds", "sequence": "contig_1", "start": 238187, "stop": 239152, "strand": "+", "frame": 2, "gene": "dnaJ", "product": "DnaJ C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E59EF3", "UniRef:UniRef90_UPI0002E59EF3" ], "nt": "TTGTTAGATCTCAACCTCTTTACTATCCCAACTAAAGCTACTAAGGATGATCTTTTGTTTGACAACATTAACCTCACTACCTATGAACAAGTTGTTGATTATCTCAACAGTCAAGCAACCCCTAATTTAGCTAAAACCGATGGTGAATTGCAAACGATTGATGGTACCAACCCATTGTTATTAGAACAGTGCAAAAAGATCAAAAAACAAGCAGAACAACTCTTTAAAAAACTCTTTTTAAAAAAACAACTCCCCTTCATCACCCAACCTGAAGTTGTTGAGGAAAGTAAAACCAGTTTTGATGAGAACAACGTTAACCTTGTTTACTTTGAAAAGGTCCCTGAAATCCTTTTCATTAACCAACAACCTAAGGAGGTAAAATACACCCGTCAAGTCTTTGATGGGTTGACAAACAAAACAACTAGTGAAACGATTACACTAGAGATCCAACTCCTCCAAACCCCAAAAGAGACTGTTAGTGCCATTTTTAAAGGCTTTGGTAATGACCATGGCAAGGGCTGTGGGGATTTAAAGATTGTTTTTGAAAAGATTAAAAGCCCCTTTTTTCAAGTCAATGAGGATGGCTTGCACTCTGCTTGCATCATTGACCCTTTAGTTGCTTACAACGGCGGGATTATCGATGTGTTTGGGCCCTACACTAACTTCCAAGTTAAGGTAGATGGGGAGATAGACATCAATGCCATTATGAAGTTTGAAAAACTAGGCATTGCTAAAACCAAGCGCAAGGGCGATCTTTTTGTCCATCTCTATTACAGTAGTGTCCCTAAAAAGAAACTCACCACTAACCCCCAAGTTCAACAGTTCTTAGAACTTTTACAAGCTGAATATGAACTGTTGCAAGACAACATCAAGAGCTTAAAGTACTTTAAAAATAACCTAGTTATCCCCAAAAAGCCACTTGATCAACAAAGCTATCAATACCTCTCCCAAGAACCCATTAGTTAG", "aa": "MLDLNLFTIPTKATKDDLLFDNINLTTYEQVVDYLNSQATPNLAKTDGELQTIDGTNPLLLEQCKKIKKQAEQLFKKLFLKKQLPFITQPEVVEESKTSFDENNVNLVYFEKVPEILFINQQPKEVKYTRQVFDGLTNKTTSETITLEIQLLQTPKETVSAIFKGFGNDHGKGCGDLKIVFEKIKSPFFQVNEDGLHSACIIDPLVAYNGGIIDVFGPYTNFQVKVDGEIDINAIMKFEKLGIAKTKRKGDLFVHLYYSSVPKKKLTTNPQVQQFLELLQAEYELLQDNIKSLKYFKNNLVIPKKPLDQQSYQYLSQEPIS", "aa_hexdigest": "3cf905c80b63c1c53a1586ea2039436e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002E59EF3", "query_cov": 1.0, "subject_cov": 0.8941504178272981, "identity": 0.997, "score": 625.0, "evalue": 1.53e-225, "valid": true, "product": "DnaJ C-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0002E59EF3" }, "pscc": { "uniref50_id": "UniRef50_UPI0002E59EF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E59EF3" ], "product": "DnaJ C-terminal domain-containing protein" }, "genes": [ "dnaJ" ], "id": "KKNAAMEMLJ_459", "locus": "GCA000027325_00423" }, { "type": "cds", "sequence": "contig_1", "start": 239164, "stop": 239616, "strand": "+", "frame": 1, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "GO:0000774", "GO:0005737", "GO:0006457", "GO:0042803", "GO:0051082", "GO:0051087", "KEGG:K03687", "SO:0001217", "UniRef:UniRef50_P47443", "UniRef:UniRef90_P47443" ], "nt": "ATGTGTGAAAAATCACAAACAATTAAAGAGCTTTTAAACGCCATTAGAACCTTAGTTGTCAAGAACAATAAAGCTAAGGTTAGTATGATTGAAAAGGAACTGTTAGCTTTTGTTAGTGAACTTGACAAAAAGTTCAAACAACAACTCAACAACTTCAATGAACTACAACAAAAGATCCCACTACTCCAAAAAGCTAACGAAGAGTTTGCTTTAAAGTTTGAAAGGATGCAACGCGAAGCACAAAACCAGATCCAAGCCAAACTAGATGAGTTGAATCTTAAAAATAAAAAGGAGTTAGAACAAGCCAAGAAATATGCGATTGCCAAAACCCTTGACCAACCCTTAAACATCATCGATCAGTTTGAAATCGCGCTTTCATATGCCCAAAAAGACCCTCAAGTAAAAAACTATACCACTGGTTTTACCATGGTACTTGATGCTTTTTCAAGGTGA", "aa": "MCEKSQTIKELLNAIRTLVVKNNKAKVSMIEKELLAFVSELDKKFKQQLNNFNELQQKIPLLQKANEEFALKFERMQREAQNQIQAKLDELNLKNKKELEQAKKYAIAKTLDQPLNIIDQFEIALSYAQKDPQVKNYTTGFTMVLDAFSR", "aa_hexdigest": "dcd1c5028af2b4cc9c5a6cc785425d1b", "start_type": "ATG", "rbs_motif": null, "genes": [ "grpE" ], "truncated": "3-prime", "pseudogene": { "start": 239164, "stop": 239817, "observations": { "mutation": [ 239614 ], "stop": [ 239614 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47443", "query_cov": 1.0, "subject_cov": 0.6912442396313364, "identity": 1.0, "score": 275.0, "evalue": 4.01e-92, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MCEKSQTIKELLNAIRTLVVKNNKAKVSMIEKELLAFVSELDKKFKQQLNNFNELQQKIPLLQKANEEFALKFERMQREAQNQIQAKLDELNLKNKKELEQAKKYAIAKTLDQPLNIIDQFEIALSYAQKDPQVKNYTTGFTMVLDAFSRWLEANGVTKIKIEPGMEFDEKIMSALELVDSNLAKNKVVRVSKSGYKLYDKVIRFASVFVSKGNKKS" }, "paralog": false, "identity": 0.9953917050691244, "score": 412.0, "evalue": 7.74e-149, "description": "Internal stop codon at 239614. Nonsense mutation around 239614.", "psc": { "uniref90_id": "UniRef90_P47443", "gene": "grpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_P47443", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687", "go_ids": [ "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47443", "UniRef:UniRef50_P47443", "COG:COG0576", "COG:O", "KEGG:K03687", "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ] }, "pscc": { "uniref50_id": "UniRef50_P47443", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47443" ], "product": "Protein GrpE" } }, "id": "KKNAAMEMLJ_460", "locus": "GCA000027325_00424" }, { "type": "cds", "sequence": "contig_1", "start": 239617, "stop": 239817, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCAAATGGGGTTACCAAGATTAAGATTGAACCAGGGATGGAATTTGATGAAAAGATTATGTCTGCATTGGAACTAGTTGATTCTAACCTTGCTAAAAACAAGGTAGTAAGAGTCTCAAAATCTGGCTATAAACTCTATGACAAAGTGATCCGCTTTGCATCAGTATTTGTCAGCAAAGGTAATAAAAAATCATAA", "aa": "MEANGVTKIKIEPGMEFDEKIMSALELVDSNLAKNKVVRVSKSGYKLYDKVIRFASVFVSKGNKKS", "aa_hexdigest": "e587f55f73e988ad5231e8e176511e67", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7367.566200000002, "isoelectric_point": 9.646807289123537 }, "id": "KKNAAMEMLJ_461", "locus": "GCA000027325_00425" }, { "type": "cds", "sequence": "contig_1", "start": 239827, "stop": 240195, "strand": "+", "frame": 1, "gene": null, "product": "MPN121 family protein", "db_xrefs": [ "RefSeq:WP_010869370.1", "SO:0001217", "UniParc:UPI0000139460", "UniRef:UniRef100_P47444", "UniRef:UniRef50_P75353", "UniRef:UniRef90_P47444" ], "nt": "ATGAGTGAACAAAAAAGAAGAACAATCCAAATTGCGATAAGTGAAGACCACTATGAAGAGTTACAAAAGGCATTGGAACTACTTAAAGGGACCCAATTACCCTTTTCAACCACTGTTGAACAGTTTGTGGAGTTAATCTTATCTAACTATGTAGCTACTTCCAATAAGATTAGTAGTTTAGCTAAGAGTGGTTTTGATGTAGCTTCATTGCAGCAAGAACTTGAGAAGATAGGTAACCTTAGTGGGGTTGATGATAACCTCAAGGGTTTTCTCTCAGAACTGTTGAAAACCTCAAGGAATGGGTTTAGTAACCCCAATAAAGATGGCAAAAAAAATGATGACGATAATAACTCGTCATCAAAATCATAG", "aa": "MSEQKRRTIQIAISEDHYEELQKALELLKGTQLPFSTTVEQFVELILSNYVATSNKISSLAKSGFDVASLQQELEKIGNLSGVDDNLKGFLSELLKTSRNGFSNPNKDGKKNDDDNNSSSKS", "aa_hexdigest": "864cae9723138c15b3bad2d2b444adbb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139460", "ncbi_nrp_id": "WP_010869370.1", "uniref100_id": "UniRef100_P47444", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139460", "RefSeq:WP_010869370.1", "UniRef:UniRef100_P47444" ] }, "ips": { "uniref100_id": "UniRef100_P47444", "uniref90_id": "UniRef90_P47444" }, "psc": { "uniref90_id": "UniRef90_P47444", "product": "MPN121 family protein", "uniref50_id": "UniRef50_P75353" }, "pscc": { "uniref50_id": "UniRef50_P75353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75353" ], "product": "Uncharacterized protein MG202 homolog" }, "genes": [], "id": "KKNAAMEMLJ_462", "locus": "GCA000027325_00426" }, { "type": "tRNA", "sequence": "contig_1", "start": 240213, "stop": 240286, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 61.8, "nt": "GCCGAATTGGTTTAGGGGCAAAACAGATCCATGGTAAGGATCAGAGAACAGTTCGACTCTGTTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 240276, 240278 ], "id": "KKNAAMEMLJ_33", "locus": "GCA000027325_00427" }, { "type": "cds", "sequence": "contig_1", "start": 240323, "stop": 242068, "strand": "+", "frame": 2, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47445", "UniRef:UniRef90_P47445" ], "nt": "ATGAAAAGTAACTACAGTGCAACTAACATCAAGATCTTAAAGGGTTTGGATGCAGTTAAAAAGCGTCCGGGGATGTACATTGGTTCTACTGATAGTAAGGGTCTGCACCACATGCTATGGGAAATTCTTGCTAACAGTGTTGATGAAGTTTTAGCTGGTTATGCAACCAATATTACTGTTACTTTAGATCTCAACAACACCATTACTGTTAGTGATGATGGCAGGGGTATTCCCTATGAGATCCACCAAGACAGTAACATCTCTACGATCGATACAGTTTTCACCTTTCTCCATGCAGGGGGGAAGTTTGATGATCAGTCATACAAACTAGCAGGGGGATTACATGGGGTTGGTGCATCAGTGGTCAATGCCTTAAGTGATCATTTAGAAGTAACAGTGAAAAGAAATGGTCAGATCTACCAATCAGTTTATCAAGCTGGGGGTAAGATCATCCAAAAAGCCAAAAAGATTGGTGATACAACTAGCCATGGTACCACTGTTAGTTTCCATGCTGACCCTAAGGTCTTTAAAAAGGCTCAATTTGATAGCAACATTATTAAAAGCAGGTTAAAAGAGCTAAGCTTTCTGTTTGCTAAACTAAAGCTCACTTTTACTGATCAAAAAACTAATAAAACCACTGTTTTTTTTAGTACCTCAGGACTAGTTCAGTTCCTTGATGAAATTAATAATACTGTAGAAACACTTGGCCAAAAAACACTGATTAAAGGTGAGAAGGATGGGATTGAAGTGGAAGTGGTTTTCCAGTTTAACCAATCAGATCAAGAGACAATCTTATCATTTGCTAACTCGATTAAAACCTTTGAAGGAGGGAGTCATGAAAATGGGTTTTGTCTTGCCATTAGTGATGTGATCAACAGCTATTGCAGAAAGTACAACTTACTAAAAGAAAAAGATAAAAACTTTCAACTTAGTGAGATCAGACAAGGGTTGAATGCTATTATCAAAGTTAACTTACCTGAAAAAAACATCGCTTTTGAAGGACAAACTAAGAGTAAGTTGTTTTCAAAGGAAGTGAAAAACGTTGTTTATGAATTGGTCCAACAACACTATTTCCAGTTTCTGGAAAGAAACAACAATGATGCTAAATTGATCATTGATAAACTACTCAATGCTAGAAAGATTAAAGAGCAAATCAAACAACAACGTGAGTTGAAAAAAAGTTTATCAAGTCCCCAAAAAGAGAAGATCTTATTTGGGAAGTTAGCACCTTGTCAAACCAAAAAAACCAGTGAAAAAGAGTTGTTTATTGTTGAAGGTGATAGTGCTGGTGGCACTGCTAAAATGGGCCGTGATAGAATTTTTCAAGCTATCTTACCTTTGCGCGGCAAGGTGTTAAATGTTGAAAAAATTAACAATAAGAAGGAAGCGATCACTAACGAAGAGATCCTCACTTTAATCTTTTGTATTGGTACAGGGATTTTAACTAACTTCAACATCAAGGACTTAAAGTACGGAAAGATCATCATTATGACTGATGCAGATAATGATGGCGCACACATCCAAATCCTCTTACTTACCTTCTTTTATAGGTACATGCAACCCTTAATTGAACTGGGCCATGTCTATCTAGCTCTTCCTCCTTTATATAAACTGGAAACCAAAGATAGAAAAACAGTTAAATACCTCTGGAGTGATTTGGAGTTGGAATCAGTCAAACTAAAGCTTAATAACTTCACTTTACAACGATACAAAGGACTTGGAGAGATGAATGCTGATCAGTTGTGA", "aa": "MKSNYSATNIKILKGLDAVKKRPGMYIGSTDSKGLHHMLWEILANSVDEVLAGYATNITVTLDLNNTITVSDDGRGIPYEIHQDSNISTIDTVFTFLHAGGKFDDQSYKLAGGLHGVGASVVNALSDHLEVTVKRNGQIYQSVYQAGGKIIQKAKKIGDTTSHGTTVSFHADPKVFKKAQFDSNIIKSRLKELSFLFAKLKLTFTDQKTNKTTVFFSTSGLVQFLDEINNTVETLGQKTLIKGEKDGIEVEVVFQFNQSDQETILSFANSIKTFEGGSHENGFCLAISDVINSYCRKYNLLKEKDKNFQLSEIRQGLNAIIKVNLPEKNIAFEGQTKSKLFSKEVKNVVYELVQQHYFQFLERNNNDAKLIIDKLLNARKIKEQIKQQRELKKSLSSPQKEKILFGKLAPCQTKKTSEKELFIVEGDSAGGTAKMGRDRIFQAILPLRGKVLNVEKINNKKEAITNEEILTLIFCIGTGILTNFNIKDLKYGKIIIMTDADNDGAHIQILLLTFFYRYMQPLIELGHVYLALPPLYKLETKDRKTVKYLWSDLELESVKLKLNNFTLQRYKGLGEMNADQL", "aa_hexdigest": "277bb6ee9e7f5a712c22257600721f8c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47445", "query_cov": 1.0, "subject_cov": 0.9178515007898894, "identity": 1.0, "score": 1124.0, "evalue": 0.0, "valid": true, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47445", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47445", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47445" ], "product": "DNA topoisomerase 4 subunit B" }, "genes": [ "parE" ], "id": "KKNAAMEMLJ_463", "locus": "GCA000027325_00428" }, { "type": "cds", "sequence": "contig_1", "start": 242078, "stop": 242224, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCAACTACCAGAAAGCTAGTGCAAGTAAAGCTTGATGATCTAATTAACGCTGAAAAGCAAATCAACATCTTTATGGGTGAAAAGAGTGATTTGCGCAAACACTGGATTGAAGCCAACATTAACTTTAGTGTGGAAAACTAA", "aa": "MNPTTRKLVQVKLDDLINAEKQINIFMGEKSDLRKHWIEANINFSVEN", "aa_hexdigest": "028fb2ef8b90c14f4f361c9eeedc31c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5642.425299999999, "isoelectric_point": 6.539689826965333 }, "id": "KKNAAMEMLJ_464", "locus": "GCA000027325_00429" }, { "type": "cds", "sequence": "contig_1", "start": 242224, "stop": 242511, "strand": "+", "frame": 1, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "nt": "ATGGATCAAAAAAACAACAACCTCTTTCAAAAGGCAATTGAAGAAGTCTTTGCAGTTAGCTTTAGTAAGTATGCTAAATACATCATCCAAGATAGAGCTTTACCTGATCTAAGAGATGGGTTAAAACCAGTACAAAGACGGATCTTATATGGGATGTTTCAAATGGGCTTAAAACCCACCACTCCCTATAAAAAATCAGCCCGTGCTGTTGGGGAGATCATGGGGAAATACCACCCCCATGGTGATAGTTCCATTTATGATGCAATTATCAGAATGTCCCAAAGCTGA", "aa": "MDQKNNNLFQKAIEEVFAVSFSKYAKYIIQDRALPDLRDGLKPVQRRILYGMFQMGLKPTTPYKKSARAVGEIMGKYHPHGDSSIYDAIIRMSQS", "aa_hexdigest": "3c5d2d43d1ff1f3ef34dfe1c73e2937b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028540D82", "query_cov": 0.8736842105263158, "subject_cov": 0.83, "identity": 0.6509999999999999, "score": 122.0, "evalue": 1.42e-34, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_Q9XCU2" }, "pscc": { "uniref50_id": "UniRef50_Q9XCU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "KKNAAMEMLJ_465", "locus": "GCA000027325_00430" }, { "type": "cds", "sequence": "contig_1", "start": 242557, "stop": 244569, "strand": "+", "frame": 1, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335", "SO:0001217", "UniRef:UniRef50_P47446", "UniRef:UniRef90_P47446" ], "nt": "GTGGATGGGGATAATGCTGCAGCAATGCGTTACACAGAAACCCGCTTAAGCTTGTATGGATTTGAACTATTAAAAGACATTGATAAAAAGTTAGTTAGTTTTATCAATAACTTTGATGATAGTGAAAAAGAACCAACGGTTTTACCAACCTTACTGCCTAACCTCTTTATCAATGGTGCGAGTGGGATAGCTGCTGGATATGCAACTAATATTGCTCCCCATAACACTAATGAACTATTAGATAGTCTTTGCTTGCGAATAGACCAACCTAATTGTGAACTTAAACAAATTTTAAAAATTGTTAAAGGTCCTGATTTTCCAACAGGGGGTAATGTTTATTTTGAAAAGAGTTTAAGTGATATTTATCAAGCAGGCAAAGGTAAATTTATTATCCAAGCTAAGTATGAAGTTAACAAGAACTTAAACCAGATTGAAATTACCCAAATCCCTTATGAAACACTGAAAGCTAACATTGTCAAACAAATTGAAGAGATTATCTTTGACAATAAACTATCTGCTATTGAAAGTGTCATTGATAGTTCAGATCGCAACGGCATTAGGATCATTATTAAACACAAGGACTTTTTGCCTGCTGAGAAGATCATGGCCTTTTTGTTTAAACACACCCAACTCCAAGTGAACTTTAACCTTAATAACACCGTGATTGCTAACCGCTTTCCCATCCAAATTGGTTTACTAAGTTACCTCGATCATTTTTTAAAGTTTTGTCATGAACTAATTATTAATAAAGCTAAGTATGAACTTGAGCTTGCAAGCAAGCGCTTGGAAATTATTTTAGGACTAATTAAAGCGATTAGTATCATTGATAAAATCATCAAATTAATTAGATCAGCAGTTGACAAAAGTGATGCAAGAGAAAAGTTAATTGATAACTTTAAATTTACTTTTAACCAAGCAGAGGCAATTGTTAGTTTGCGACTTTACCAACTAACTAACACTGATATTTTTGAACTTAACCAAGAACAAAATGAACTTGAAAAAACTGTGATTAGTTCAGAGCAACTAATTGCTAGTGAAAAAGCAAGAAACAAACTCCTAAAAAAACAGTTTGAAGGTTATAAAAAGCAGTTTCACCAGCAACGAAGGTCACAAATATGTGGCTTTATTAACCAAAAAAAGGTGGAGGAAAGTGAGCTAATTGAAAACAAAACTTATGGGGTTTTAATCACTAAAGCTGGTAACTACCATAAGTTTGAATCTAACCAACTATTAAAAAGCACCACTGATTTTAAAAGTGAGAGTGACACAATTATCTTTGCACAAACTATTGCTAATACCGACCAAATTTTTATTGTCACTTCACTAGGTAACATTATTAATATCCCTGTTTATAAATTAGCTTTCAATTCCAAAAATAAACTAGCAAGTTTAGTTAGTAAAAAACCAATCCTTTTGGAGTATGAAACGATTGTTTTTGTTGGAACAATGAACAGTGTAAACCAACCAATCCTTGTTTTAACTTCCAAACTAGGAATGGTTAAACGGATTGATTTAACCAAACTTAACATTAAGCCACTTAAAGCTACTTTGTGTATCTCACTCCGTGATAAAGACCATTTAGTAAGTGCATTTTTACAACAAGATGATAAACTGATCTGTTTAGTGTCTGATCACAACTATTACACTGTTTTTCACACCAATGAGATCCCATTAATTAGTAGTAAGGGGATGGGAGTGAAGGGGATGAAGTTAAAACTAGAGGATCAAATTAAGTTTGTTGTTGCTTTTGAAGCTAATGAACCGTTAGTGATGATATGTAGTGATGGTAGTGTCATTAACTTAAAACAAACTGAACTAGTTGTAGTTAGCAGGATGGCAACTGCAAAAAAACTGCCTGTTAAGAAAGCAATTAACTATTGTTTTAGTGATGCAACTAACACCCAGTTAATTAATTTTCAGGGTAAGAACGGTAGTAAATTAATTACAACTAGTGAACTGAACCAGATGAGTAAAACTGCAATTAGTCAAACCAGGTTTAACAAACTTAATTAG", "aa": "MDGDNAAAMRYTETRLSLYGFELLKDIDKKLVSFINNFDDSEKEPTVLPTLLPNLFINGASGIAAGYATNIAPHNTNELLDSLCLRIDQPNCELKQILKIVKGPDFPTGGNVYFEKSLSDIYQAGKGKFIIQAKYEVNKNLNQIEITQIPYETLKANIVKQIEEIIFDNKLSAIESVIDSSDRNGIRIIIKHKDFLPAEKIMAFLFKHTQLQVNFNLNNTVIANRFPIQIGLLSYLDHFLKFCHELIINKAKYELELASKRLEIILGLIKAISIIDKIIKLIRSAVDKSDAREKLIDNFKFTFNQAEAIVSLRLYQLTNTDIFELNQEQNELEKTVISSEQLIASEKARNKLLKKQFEGYKKQFHQQRRSQICGFINQKKVEESELIENKTYGVLITKAGNYHKFESNQLLKSTTDFKSESDTIIFAQTIANTDQIFIVTSLGNIINIPVYKLAFNSKNKLASLVSKKPILLEYETIVFVGTMNSVNQPILVLTSKLGMVKRIDLTKLNIKPLKATLCISLRDKDHLVSAFLQQDDKLICLVSDHNYYTVFHTNEIPLISSKGMGVKGMKLKLEDQIKFVVAFEANEPLVMICSDGSVINLKQTELVVVSRMATAKKLPVKKAINYCFSDATNTQLINFQGKNGSKLITTSELNQMSKTAISQTRFNKLN", "aa_hexdigest": "f21c8a9988f48d64479f905d741ffc08", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47446", "query_cov": 1.0, "subject_cov": 0.8578745198463509, "identity": 0.9990000000000001, "score": 1249.0, "evalue": 0.0, "valid": true, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47446", "cog_id": "COG0188", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47446", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47446" ], "product": "DNA topoisomerase 4 subunit A" }, "genes": [ "parC" ], "id": "KKNAAMEMLJ_466", "locus": "GCA000027325_00431" }, { "type": "cds", "sequence": "contig_1", "start": 244566, "stop": 244796, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTCATAAACCAAACCAACCAAAAGACCAACATTGTCTTTGGTGATCAATTGGGTTTTAAAGAGATATCAGTTGCTTCTACTTTAATCAATACCACCAGTGAAGCCAAGCACCAACTTGCTATAGTAGGACCAACCAGAATGGACTACCAAAAGATTAAAGCCTTATTAACTACTCTAAAAGAAGAGATAGAAAAATATGATAAAAAGATCCACAACCAAACCTAA", "aa": "MAFINQTNQKTNIVFGDQLGFKEISVASTLINTTSEAKHQLAIVGPTRMDYQKIKALLTTLKEEIEKYDKKIHNQT", "aa_hexdigest": "09cf3b39302cca20f7123ef5ddd3eaaa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8638.835499999997, "isoelectric_point": 9.005153465270997 }, "id": "KKNAAMEMLJ_467", "locus": "GCA000027325_00432" }, { "type": "cds", "sequence": "contig_1", "start": 244803, "stop": 245597, "strand": "-", "frame": 1, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "GO:0045892", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_P75351", "UniRef:UniRef90_P47447" ], "nt": "ATGAAGAATTTAACGCCACGGCAAGCCCAAATTCTCAAAGCAATTATCAATGAATACATTGCTTATGCTATCCCTGTTGGCAGTAAATTACTAACCAAAAAATATTTTAAAAACCTCTCTGGGGGAACATTACGAAATGAGATGGCAGCTTTAGAGAAAAAAGGGTTCTTAAAGAAAAACCACATCTCTTCAGGCCGTGTTCCTTCCCAAATTGGTTATCAGTATTATGTCAAAGTGTTAAATGTTAGTAACACCACTAACGATCTGAAAACCCGCTTAAGAAGTGTTATTCTCCAGCAACACAAAACGATTGATGAGGTGATTGAACTTGGGGTTAAGTTTATCAATGAGATCATTAACCTACCAGTAGTGTTAACTAACTTTAGTAGTGATGAAGTTTTAAAAAAGATTGATCTGATCATCCTTGATAAGTCATTTGCCTTATTTTTATTGGTCTCCGCTAGTGGTAAAGTCTTTAAAAAAACCATTAGCTATGCTAACCAAAGGCAGTTTGAAGACATTGTTATTTGTGTAAGGATCTTTAATGATCGCATCATTGATACCCGCTTTTCAGAGATTAACAACCAACTAGAAGTTTTAAAAGAGATCATTAGAACCAAGGTTCATGAGTATCAGTATGTAATTGATGAGATCTTATTTAAGTTATTTGATTTAGATCAAATTGAGGCAAATAAAAAGATCTATGGGATCCAATACCTTGCTAAACAACCCGAGTTTGCTAACCAAGAGAAACTCACTAAAATCTTAAATCTGCTTGAAGATACTAGTGTTTGA", "aa": "MKNLTPRQAQILKAIINEYIAYAIPVGSKLLTKKYFKNLSGGTLRNEMAALEKKGFLKKNHISSGRVPSQIGYQYYVKVLNVSNTTNDLKTRLRSVILQQHKTIDEVIELGVKFINEIINLPVVLTNFSSDEVLKKIDLIILDKSFALFLLVSASGKVFKKTISYANQRQFEDIVICVRIFNDRIIDTRFSEINNQLEVLKEIIRTKVHEYQYVIDEILFKLFDLDQIEANKKIYGIQYLAKQPEFANQEKLTKILNLLEDTSV", "aa_hexdigest": "681cc5b1d1a7ea27d7e8294c69d49085", "start_type": "ATG", "rbs_motif": null, "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 244566, "stop": 245597, "observations": { "mutation": [ 244805 ], "stop": [ 244805 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47447", "query_cov": 1.0, "subject_cov": 0.7696793002915452, "identity": 1.0, "score": 503.0, "evalue": 1.68e-178, "gene_start": 1, "gene_end": 264, "reference_start": 1, "reference_end": 264, "reference_sequence": "MKNLTPRQAQILKAIINEYIAYAIPVGSKLLTKKYFKNLSGGTLRNEMAALEKKGFLKKNHISSGRVPSQIGYQYYVKVLNVSNTTNDLKTRLRSVILQQHKTIDEVIELGVKFINEIINLPVVLTNFSSDEVLKKIDLIILDKSFALFLLVSASGKVFKKTISYANQRQFEDIVICVRIFNDRIIDTRFSEINNQLEVLKEIIRTKVHEYQYVIDEILFKLFDLDQIEANKKIYGIQYLAKQPEFANQEKLTKILNLLEDTSVWQQMAFINQTNQKTNIVFGDQLGFKEISVASTLINTTSEAKHQLAIVGPTRMDYQKIKALLTTLKEEIEKYDKKIHNQT" }, "paralog": false, "identity": 0.9970845481049563, "score": 657.0, "evalue": 6.79e-242, "description": "Internal stop codon at 244805. Nonsense mutation around 244805.", "psc": { "uniref90_id": "UniRef90_P47447", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_P75351", "cog_id": "COG1420", "cog_category": "K", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355", "GO:0045892" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47447", "UniRef:UniRef50_P75351", "COG:COG1420", "COG:K", "KEGG:K03705", "GO:0003677", "GO:0006355", "GO:0045892" ] }, "pscc": { "uniref50_id": "UniRef50_P75351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75351" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "KKNAAMEMLJ_468", "locus": "GCA000027325_00433" }, { "type": "cds", "sequence": "contig_1", "start": 245966, "stop": 247426, "strand": "+", "frame": 2, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "COG:COG0322", "COG:L", "GO:0003677", "GO:0005737", "GO:0006289", "GO:0006974", "GO:0009380", "GO:0009381", "GO:0009432", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_P47448", "UniRef:UniRef90_P47448" ], "nt": "TTGGAATTAGCCAGAAAATACCAGATTAAAACCACTAAGTGCTTTGGACCGTTTGCTTCAAGTAAGTTTAAGTTACGTGAGATCCATGACCTACTTTTAAAACTCTTTCCTTTAAGAAAGTGTGCACCTCACCAAAAAAACCACCCGTGCTTTTATTTTCAGATGGGTTTATGTATGGGGCAATGTATGCAAACTGATACTAAGGAAAAATACCAACAGGTAATTAGTAACATTGAACAGTTTTTTAATGACCCTAGTGTGGTAATTAACTATTTAAAAGCTGCAGAAAAAAAGGCAAGTGATAATCAGGAATTTGAAAAGGCCCAGCAGTTTCTAACACTGCAAAAAGCAGTTTTAGAGTTAACAAAAACCCACCATACCACTATCATTAAACAAAAATCAAGCCATGATTTTATTGGGTATGTCTTTCAAAATAACGTTTTGGCCATTACCATTTTTTGTTATGAAAAAGGGGAGTTAACTGATAAAGAACAAGCAGTGTTTACCCTAGAGCAAACTGACATTGTGGAAGTTGAAAGTGCTATTATCACCTTTATCTACCACCACTATAAAACTACCCCACTTCCAAGTAAGATTACTGTTTCACTTGATGAAACTAACCTAAAACTTATTAGTGATAGCTTAAAAATTGGTGTTTTTAAGCCCAAGAATGGTAATGAAAAACTGATCTTACAAACTGTTATTGATAATGCCAAACATGCACTTGCAACCAAGTGGTTGAAGTTTACTAGTAACTATGATAAAACCCAGCTCCACAAGGATTTAGCACAACTTCTAAATACTGATTATATCCATAGTCTTGAGATTATTGATGTGTCATTCTATGATCAAAACCATGTTGTTGGTTGCATGTTAAGGTTTGAAGATGGTAAAAAGATCAAACACTTATCAAGAAGATACAACATTAACAGTTTAAAAAAAGGTGATACTAACCACATTGCTTTACTTGTTTACAGAAGGATCTTAAGTGCGATGCAAACCAAAGCTAACCTCCCTTTTAGTGATCTTTTAATTATTGATGGTGGTAAAGCACAAATTAAAAGTGTTAAGCAAGTTTTTAGTCTCTTCAGTAATGTTAAACCACCCATTATCATTGGACTAGTTAAAAACAAAAACCACCAAACTGATCACATTATGTTATCTGATTTCCAAGTTAAAAAGATAGCAATTAACTCCCCACTCTTTCACTATTTAGCAACAATCCAAACTGAAGTTGATGGTTTTGCTAAAAGAAGTGCTTTTAATAAGTTAAGTAACCACCAACTGCAAAACCCGTTGCTACAAATCCCAGGAGTTGGCAAGATAACTGCCCAAATTCTCTTTGATAACTTTCAAACGCTCAATAACATAAAATTAGCTTCAGTTAATGAGTTAAGCCAGTTTATTAAAAAACCATTAGCACAAAAGATTAAAACTTACTTTGCAAAACAAACTGATTAA", "aa": "MELARKYQIKTTKCFGPFASSKFKLREIHDLLLKLFPLRKCAPHQKNHPCFYFQMGLCMGQCMQTDTKEKYQQVISNIEQFFNDPSVVINYLKAAEKKASDNQEFEKAQQFLTLQKAVLELTKTHHTTIIKQKSSHDFIGYVFQNNVLAITIFCYEKGELTDKEQAVFTLEQTDIVEVESAIITFIYHHYKTTPLPSKITVSLDETNLKLISDSLKIGVFKPKNGNEKLILQTVIDNAKHALATKWLKFTSNYDKTQLHKDLAQLLNTDYIHSLEIIDVSFYDQNHVVGCMLRFEDGKKIKHLSRRYNINSLKKGDTNHIALLVYRRILSAMQTKANLPFSDLLIIDGGKAQIKSVKQVFSLFSNVKPPIIIGLVKNKNHQTDHIMLSDFQVKKIAINSPLFHYLATIQTEVDGFAKRSAFNKLSNHQLQNPLLQIPGVGKITAQILFDNFQTLNNIKLASVNELSQFIKKPLAQKIKTYFAKQTD", "aa_hexdigest": "f31a66cbd6605ceeb8450593e39da865", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47448", "query_cov": 1.0, "subject_cov": 0.8140703517587939, "identity": 0.998, "score": 958.0, "evalue": 0.0, "valid": true, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_P47448", "cog_id": "COG0322", "cog_category": "L", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0006974", "GO:0009380", "GO:0009381", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P47448", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47448" ], "product": "UvrABC system protein C" }, "genes": [ "uvrC" ], "id": "KKNAAMEMLJ_469", "locus": "GCA000027325_00434" }, { "type": "cds", "sequence": "contig_1", "start": 247407, "stop": 247565, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00149.33" ], "nt": "TTGCAAAACAAACTGATTAAGGTTTTGGTAATTGCTGATACCCATGGTCAAAACCAGAGGTGGATTGAACTGAAAAACTACCATAACCCTGATGTGATTATCCATGCAGGAGATCACATGACCACTAAACAATTCATGGATCAAAATGCTACTTTTTGA", "aa": "MQNKLIKVLVIADTHGQNQRWIELKNYHNPDVIIHAGDHMTTKQFMDQNATF", "aa_hexdigest": "de0b7a5eb7188aeeb12b65db36c68253", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00149.33", "name": "Metallophos", "length": 42, "aa_cov": 0.6923076923076923, "hmm_cov": 1.0, "evalue": 5.0969488596892605e-08, "score": 28.64311981201172, "start": 6, "stop": 41 } ], "seq_stats": { "molecular_weight": 6104.909600000002, "isoelectric_point": 6.894762992858888 }, "id": "KKNAAMEMLJ_470", "locus": "GCA000027325_00435" }, { "type": "cds", "sequence": "contig_1", "start": 247566, "stop": 247910, "strand": "+", "frame": 3, "gene": "yfcE", "product": "putative metallophosphoesterase MG207", "db_xrefs": [ "COG:COG0622", "COG:R", "EC:3.1.4.-", "GO:0016787", "GO:0046872", "KEGG:K07095", "SO:0001217", "UniRef:UniRef50_P47449", "UniRef:UniRef90_P47449" ], "nt": "GTGGCAGGTAACAACGATTCAATTGGCAATGAAATTGAAATTTTCCAGTTAGGGCAAATTAACTTTGTGTTAATGCATGGTCACCAAGCACCAAGAGATAACCTGAAAAAGTGGTACCAATTATTAGTTTTAAAAGCACAACAATACCCTTGTGATGTTTTAATCTTTGGTCATAGTCACATTGAATATACCAACAAAATTAATATGATCCAGTTAATCAACCCTGGTTCTCTACAACTACCAAGAAACCAAACCAACACCCCTTCATACTGTACCTTTATTGTCAATAAAGACGAGCTAACTGATCTAACTATCCACTATTACCAAGCTTCAAAAGTTAGTTAA", "aa": "MAGNNDSIGNEIEIFQLGQINFVLMHGHQAPRDNLKKWYQLLVLKAQQYPCDVLIFGHSHIEYTNKINMIQLINPGSLQLPRNQTNTPSYCTFIVNKDELTDLTIHYYQASKVS", "aa_hexdigest": "74577a3282b3fd51b2ba120446ad0664", "start_type": "GTG", "rbs_motif": null, "genes": [ "yfcE" ], "truncated": "5-prime", "pseudogene": { "start": 247419, "stop": 247910, "observations": { "mutation": [ 247563 ], "stop": [ 247563 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47449", "query_cov": 1.0, "subject_cov": 0.6993865030674846, "identity": 0.991, "score": 238.0, "evalue": 6.24e-79, "gene_start": 1, "gene_end": 114, "reference_start": 50, "reference_end": 163, "reference_sequence": "MIKVLVIADTHGQNQRWIELKNYHNPDVIIHAGDHMTTKQFMDQNATFWVAGNNDSIGNEIEIFQLGQINFVLMHGHQAPRDNLKKWYQLLVLKAQQYPCDVLIFGHSHIEYTNKINMIQLINPGSLQLPRNQTNTPSYCTFIVNKDELTDLTIHYYQASKVS" }, "paralog": false, "identity": 0.9877300613496932, "score": 336.0, "evalue": 2.77e-120, "description": "Internal stop codon at 247563. Nonsense mutation around 247563.", "psc": { "uniref90_id": "UniRef90_P47449", "gene": "YfcE", "product": "Probable metallophosphoesterase MG207", "ec_ids": [ "3.1.4.-" ], "uniref50_id": "UniRef50_P47449", "cog_id": "COG0622", "cog_category": "R", "kegg_orthology_id": "K07095", "go_ids": [ "GO:0016787", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47449", "EC:3.1.4.-", "UniRef:UniRef50_P47449", "COG:COG0622", "COG:R", "KEGG:K07095", "GO:0016787", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47449", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47449" ], "product": "Probable metallophosphoesterase MG207" } }, "id": "KKNAAMEMLJ_471", "locus": "GCA000027325_00436" }, { "type": "cds", "sequence": "contig_1", "start": 247903, "stop": 248493, "strand": "-", "frame": 3, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "COG:COG1214", "COG:J", "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_009885745.1", "SO:0001217", "UniParc:UPI0000139473", "UniRef:UniRef100_P47450", "UniRef:UniRef50_P47450", "UniRef:UniRef90_P47450" ], "nt": "ATGTTCTTTTTAAGTAAGTATAAGCTGTTTTTAGACTGTGCTTATAAAACCCTTAACATCATTATTTTAGAGATGAAAACCAATGCAGTGGTTGATGAGTTGTCCATTGGTGTTGAACAGAACTTAACTGAACTTGCTGTTTATTATCTGGAGACAATGTTAACAAAAAACAAGTTGAAAAAAAGCAGTATTAAACAGTTTTATGTCACAATTGGCCCTGGTAGTTTCACCGGTCAGAGGATAGCAACTATCATTGCTAAAAGCTGGTGTTTATTGTATCCCAGTTGTGAACTTTATGCTTTAAATTCACTCCGCTTTCAAATCCCATATGAACATGGGATTAGCAAGATTAGTTGTGGTAATGATCAGAACTATTGTGGACTATACAGCCAAACTACTAGTGAAATAAAGCTAATTAGTAAAGCTGATTTTGTGAAGTTATGTAAGGCAAACAATGAACTTCCTATGTATGAAAACTTTGAAAACATTGAGAGTTATAGCAAGCTGTTATTAAGCAACATCGATCACTTTGAAAGAATCGAAGATCCTCTAACCTTACAACCTATCTATCTAAAAGATCCTGTTAACTAA", "aa": "MFFLSKYKLFLDCAYKTLNIIILEMKTNAVVDELSIGVEQNLTELAVYYLETMLTKNKLKKSSIKQFYVTIGPGSFTGQRIATIIAKSWCLLYPSCELYALNSLRFQIPYEHGISKISCGNDQNYCGLYSQTTSEIKLISKADFVKLCKANNELPMYENFENIESYSKLLLSNIDHFERIEDPLTLQPIYLKDPVN", "aa_hexdigest": "99d7d0675e50dd4756b8ad803e1c0ca3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139473", "ncbi_nrp_id": "WP_009885745.1", "uniref100_id": "UniRef100_P47450", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139473", "RefSeq:WP_009885745.1", "UniRef:UniRef100_P47450" ] }, "ips": { "uniref100_id": "UniRef100_P47450", "uniref90_id": "UniRef90_P47450" }, "psc": { "uniref90_id": "UniRef90_P47450", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P47450", "cog_id": "COG1214", "cog_category": "J", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_P47450", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47450" ], "product": "Uncharacterized protein MG208" }, "genes": [ "tsaB" ], "id": "KKNAAMEMLJ_472", "locus": "GCA000027325_00437" }, { "type": "cds", "sequence": "contig_1", "start": 248477, "stop": 249403, "strand": "-", "frame": 2, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG209", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0009982", "GO:0120159", "KEGG:K06180", "RefSeq:WP_009885746.1", "SO:0001217", "UniParc:UPI0000139478", "UniRef:UniRef100_P47451", "UniRef:UniRef50_P47451", "UniRef:UniRef90_P47451" ], "nt": "ATGAAACAGTGTTTTGTTGTTACAACTACCAAACGCTTAGATAGTCTTTTAGCTAGCTTACTGAACCTTTCAAGAGTAAAGGTAGTGAAGCTGATCATGAATGGACAGATTAAAGTTAATGAAAAACTAACTTTTAAAAACAGTTTAATAGTTGCAAAAGATGATGTAATTAAAGTTGAGATTCATGATGAGACAACTAGTGATTTCATTACTAGTGTTGAACCTTATAACTTAAAGCTTGAGGTTCTTTTTGAAGACAAGGATTTGATGGTTATTAACAAACCATCAGGTTTGTTAACCCATCCCACCACTTTCAATGAAAAAGCCAGCTTGTTAGCTGCTTGTATCTTTCACAACAACAAAAACCCTGTTTACTTAGTGCACAGATTGGACCGTGATACTAGTGGGGCAATTGTTGTCTGTAAAAACCAAGCAAGCTTATTAAATTTGCAAAATCAACTGCAAAATCGCACCTTAAAACGTTATTATGTAGCACTAGTCCACTTCCCTTTTAATGCCTTAACTGGTTCAATTAATGCACCTTTAGCAAGGGTTAATAACAACAAGGTAATGTTTAAAATAGCCCAAACTGCTAAAGCAAAGCAAGCAATAACTAAGTTTAAAGTGATTAATCAGAATGAAAAAGCAGCACTAATTAGCTTGGAATTGTTAACAGGTAGAACCCACCAAATTAGAGTGCATCTGAAATTTATCCAACATCCAGTTTATAATGATCCACTGTATGGAATTAAAAGTGAAAAGAAAGATAGCTATGGTCAGTTTCTCCATGCAAACAGGATCTGTTTTATCCATCCCACTTTAAACAAACCTATGGACTTTCACGCCCCACTTGAACCTAAGTTTTCAACGAAACTTAAGAGTTTAAACTTATCTTTAACCGATCCACTCCATGTTCTTTTTAAGTAA", "aa": "MKQCFVVTTTKRLDSLLASLLNLSRVKVVKLIMNGQIKVNEKLTFKNSLIVAKDDVIKVEIHDETTSDFITSVEPYNLKLEVLFEDKDLMVINKPSGLLTHPTTFNEKASLLAACIFHNNKNPVYLVHRLDRDTSGAIVVCKNQASLLNLQNQLQNRTLKRYYVALVHFPFNALTGSINAPLARVNNNKVMFKIAQTAKAKQAITKFKVINQNEKAALISLELLTGRTHQIRVHLKFIQHPVYNDPLYGIKSEKKDSYGQFLHANRICFIHPTLNKPMDFHAPLEPKFSTKLKSLNLSLTDPLHVLFK", "aa_hexdigest": "07f74059485ad672f0972c9e1a18256b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139478", "ncbi_nrp_id": "WP_009885746.1", "uniref100_id": "UniRef100_P47451", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139478", "RefSeq:WP_009885746.1", "UniRef:UniRef100_P47451" ] }, "ips": { "uniref100_id": "UniRef100_P47451", "uniref90_id": "UniRef90_P47451" }, "psc": { "uniref90_id": "UniRef90_P47451", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG209", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47451", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180", "go_ids": [ "GO:0009982", "GO:0003723", "GO:0120159", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47451", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47451" ], "product": "Uncharacterized RNA pseudouridine synthase MG209" }, "genes": [ "rluA" ], "id": "KKNAAMEMLJ_473", "locus": "GCA000027325_00438" }, { "type": "cds", "sequence": "contig_1", "start": 249610, "stop": 249948, "strand": "-", "frame": 3, "gene": "lspA", "product": "signal peptidase II", "db_xrefs": [ "COG:COG0597", "COG:MU", "EC:3.4.23.36", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306", "KEGG:K03101", "SO:0001217", "UniRef:UniRef50_P75484", "UniRef:UniRef90_Q49401" ], "nt": "ATGAAATTAAGAAAAACCAAGTTTTTTTCACAACTTAAACACCAGGTTTTAACTGCAAACCAAAAACCATTTTTATTCTATAAACTGACAATGATTGGGTTTGTTGGCTTTATTATCTTACTGCAAGTTTTCATATTAAGAAATGCGTTAAATGGTGAGATGGATAACACCATGGTAGCAAATAGTGGTTTTATTAATATCTATGTGATTAGAAACAAAGGGGTAGGGTTTAGCTTATTACAAAACCAAACTGGCTTAGTTTACTTTCTCCAGGGATTATTATCAGTAATTGCGTTAGTTTTTCTTGTTTTTATGGTGAAATATAGTTACATCTTTTGA", "aa": "MKLRKTKFFSQLKHQVLTANQKPFLFYKLTMIGFVGFIILLQVFILRNALNGEMDNTMVANSGFINIYVIRNKGVGFSLLQNQTGLVYFLQGLLSVIALVFLVFMVKYSYIF", "aa_hexdigest": "d57fe133b1b7b575ef9c508d83493b3e", "start_type": "ATG", "rbs_motif": null, "genes": [ "lspA" ], "truncated": "5-prime", "pseudogene": { "start": 249403, "stop": 249948, "observations": { "mutation": [ 249612 ], "stop": [ 249612 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49401", "query_cov": 1.0, "subject_cov": 0.6187845303867403, "identity": 1.0, "score": 207.0, "evalue": 1.61e-66, "gene_start": 1, "gene_end": 112, "reference_start": 1, "reference_end": 112, "reference_sequence": "MKLRKTKFFSQLKHQVLTANQKPFLFYKLTMIGFVGFIILLQVFILRNALNGEMDNTMVANSGFINIYVIRNKGVGFSLLQNQTGLVYFLQGLLSVIALVFLVFMVKYSYIFWITTLAFGSLGNFFDRLTSANDSVLDYFIFQNGSSVFNFADCCITFGFIGLFFCFLIQMFKEFKHSKNQ" }, "paralog": false, "identity": 0.994475138121547, "score": 358.0, "evalue": 1.46e-128, "description": "Internal stop codon at 249612. Nonsense mutation around 249612.", "psc": { "uniref90_id": "UniRef90_Q49401", "gene": "lspA", "product": "signal peptidase II", "ec_ids": [ "3.4.23.36" ], "uniref50_id": "UniRef50_P75484", "cog_id": "COG0597", "cog_category": "MU", "kegg_orthology_id": "K03101", "go_ids": [ "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49401", "EC:3.4.23.36", "UniRef:UniRef50_P75484", "COG:COG0597", "COG:MU", "KEGG:K03101", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ] }, "pscc": { "uniref50_id": "UniRef50_P75484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75484" ], "product": "Lipoprotein signal peptidase" } }, "id": "KKNAAMEMLJ_474", "locus": "GCA000027325_00439" }, { "type": "cds", "sequence": "contig_1", "start": 250551, "stop": 250796, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTAGTTGGTTTACCCTTTGAAAAAGGTGCTTATCATAAGCAGTTATATGACCATTCACGTCAGGAGATCTTAGATCTTACTGAAGTTATTAAAAAGCTTTATCTAAAAACAGAGACCAACAACGATAAGCTTGAGTTTGCTGCAACTACTAGTAAAACAACAGCGCAGCTAACTAAAACTATGCCCTTAAACAGTAGTGATCTTGATAAGGATCTAATGGAGTTTAGTGGTGAGAAGTGA", "aa": "MILVGLPFEKGAYHKQLYDHSRQEILDLTEVIKKLYLKTETNNDKLEFAATTSKTTAQLTKTMPLNSSDLDKDLMEFSGEK", "aa_hexdigest": "9b80defb3d37c571d723cb3422c4fe48", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9266.492899999997, "isoelectric_point": 5.603665351867675 }, "id": "KKNAAMEMLJ_475", "locus": "GCA000027325_00440" }, { "type": "cds", "sequence": "contig_1", "start": 250808, "stop": 250996, "strand": "+", "frame": 2, "gene": "rpsU", "product": "30S ribosomal protein S21", "db_xrefs": [ "COG:COG0828", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_009885749.1", "SO:0001217", "UniParc:UPI0000134E7C", "UniRef:UniRef100_P57085", "UniRef:UniRef50_Q8EUK4", "UniRef:UniRef90_P57079" ], "nt": "GTGCCTAAGATTGAAGTTAAAAATGATGATTTAGAGCTAGCTTTAAAAAAGTTTAAAAGGATATCACTGGAAGTACGCAGGTTAGCACAACGCCATGAATACCACTTGCGCAAAGGGATGCGGTTAAGAGAAAAACAAAAGATAGCACAGAAAAAGCGCAGGAAGTTTCGCAGTTTAGCTAGCCATTAA", "aa": "MPKIEVKNDDLELALKKFKRISLEVRRLAQRHEYHLRKGMRLREKQKIAQKKRRKFRSLASH", "aa_hexdigest": "9ff4ed1fff707dabf05dea47bbb375a5", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E7C", "ncbi_nrp_id": "WP_009885749.1", "uniref100_id": "UniRef100_P57085", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E7C", "RefSeq:WP_009885749.1", "UniRef:UniRef100_P57085" ] }, "ips": { "uniref100_id": "UniRef100_P57085", "uniref90_id": "UniRef90_P57079" }, "psc": { "uniref90_id": "UniRef90_P57079", "gene": "rpsU", "product": "30S ribosomal protein S21", "uniref50_id": "UniRef50_Q8EUK4", "cog_id": "COG0828", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EUK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EUK4" ], "product": "Small ribosomal subunit protein bS21" }, "genes": [ "rpsU" ], "id": "KKNAAMEMLJ_476", "locus": "GCA000027325_00441" }, { "type": "cds", "sequence": "contig_1", "start": 250998, "stop": 251396, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG211", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47453", "UniRef:UniRef90_P47453" ], "nt": "ATGGATAATAAAAACCCCCAGAAACTTATTACTAGTGAATTGTTGGCAAACCACCGCTTTAATTTTGCTAAAGATGATAAAGGTGGGTATGATGCTAATGAAGTTGATGCATTCTTAGATCAACTAACCAAGACTTTAATCCACTATGAGGAGATGAAAAACAACGAACAAGAATTGAAAAATGCTTATGACAAGTTGTTTTCAGATCGTGATCAGATTTTAAGTCGTTGTGCTAAATTAGAAGCTGATTTAAACACCTTTTATGAAAATGGTTATGCAAACAAGGTGTTAATTAACCGGGTTCAGGAGTTGGAGGATAAACTTGAAAAACTACCTGATCGTTACACTGAAAAACTAGAAAGGATTGAAAAACTGTTAAAAAAGGTCATTAAACACTGA", "aa": "MDNKNPQKLITSELLANHRFNFAKDDKGGYDANEVDAFLDQLTKTLIHYEEMKNNEQELKNAYDKLFSDRDQILSRCAKLEADLNTFYENGYANKVLINRVQELEDKLEKLPDRYTEKLERIEKLLKKVIKH", "aa_hexdigest": "e87fa732e2b19cb9f0e9d399a42f753a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47453", "query_cov": 1.0, "subject_cov": 0.8979591836734694, "identity": 1.0, "score": 258.0, "evalue": 6.36e-87, "valid": true, "product": "Uncharacterized protein MG211", "uniref50_id": "UniRef50_P47453" }, "pscc": { "uniref50_id": "UniRef50_P47453", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47453" ], "product": "Uncharacterized protein MG211" }, "genes": [], "id": "KKNAAMEMLJ_477", "locus": "GCA000027325_00442" }, { "type": "cds", "sequence": "contig_1", "start": 251444, "stop": 251560, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTTGGGATAGGGATTGATGTTGTGCAATTAAAGCGCTTCTTAACTTTAGTTGAAACTAGTGATTGTTTTGCTAAACGATTGTTAACTAGCAATGAACTAAACAGTTATTGA", "aa": "MVVGIGIDVVQLKRFLTLVETSDCFAKRLLTSNELNSY", "aa_hexdigest": "a13dc5c3893cda2b5abaf11f801c8775", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4273.9681, "isoelectric_point": 5.968969535827637 }, "id": "KKNAAMEMLJ_478", "locus": "GCA000027325_00443" }, { "type": "cds", "sequence": "contig_1", "start": 251781, "stop": 251975, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAACTATTTAAAACAAGTTTTAGATTCATAATAAGGTTTTTACAAATCCTGAGTTTACCAGTTGTTTTTCCTTACTTTTTATTAAGCTTTTTAGCTTGTTTAATTACTAGTAAAAACTATGAATCACTCCCTTATAACTATCCCCCTGAAATCCGATTCAAAAAGGTGTATAGATTGGTATCAATGTGA", "aa": "MDKLFKTSFRFIIRFLQILSLPVVFPYFLLSFLACLITSKNYESLPYNYPPEIRFKKVYRLVSM", "aa_hexdigest": "0580a8defe65587357de48bb24e59c8b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7747.274199999999, "isoelectric_point": 9.841888618469238 }, "id": "KKNAAMEMLJ_479", "locus": "GCA000027325_00444" }, { "type": "cds", "sequence": "contig_1", "start": 252000, "stop": 252587, "strand": "+", "frame": 3, "gene": "plsC", "product": "putative 1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "EC:2.3.1.51", "GO:0003841", "GO:0006654", "GO:0016020", "GO:0016024", "SO:0001217", "UniRef:UniRef50_Q49402", "UniRef:UniRef90_Q49402" ], "nt": "GTGACAGTAAATGACAAGATTATCCCTAAAAAACCAGTTTTAGTGGTAGCTAACCACAAATCTAACCTTGATCCTTTAGTATTAATTAAGGCCTTTGGCAGGTTGAAAAATAGTCCACCATTAACCTTTGTTGCTAAGATTGAACTGAAAGATACAGTCCTTTTTAAACTGATGAAATTAATTGATTGTGTTTTTATTGATCGAAAAAACATCAGACAAATTGCCAATGCATTGGAAACCCAACAACAACTAATTCGCCAGGGCACTGCTATTGCTGTTTTTGCTGAAGGGACTAGGATTTTAAGTAATGACATTGGGGAATTTAAACCAGGAGCACTAAAGGTTGCTTACAATGCTTTTGTACCTATCTTACCAGTTAGTATTGTGGGTAGCTTAGGAAAGATGGAATCAAACAAAAGGCTAAAAGAACATGGTGTTAAGAAAAGTTCAAACTATGAGGTTAAAGTAATCTTTAACAAGCTAATTAACCCAATTAGTTTTAACCAGATTGATTCTAATAACCTTGCTAATAACATTAGAAGCATTATTAGTGATGCATACACTAGTGAAAAACCAAGCAATGATTAG", "aa": "MTVNDKIIPKKPVLVVANHKSNLDPLVLIKAFGRLKNSPPLTFVAKIELKDTVLFKLMKLIDCVFIDRKNIRQIANALETQQQLIRQGTAIAVFAEGTRILSNDIGEFKPGALKVAYNAFVPILPVSIVGSLGKMESNKRLKEHGVKKSSNYEVKVIFNKLINPISFNQIDSNNLANNIRSIISDAYTSEKPSND", "aa_hexdigest": "622239409f0f6bb20e12654bc302dbb7", "start_type": "GTG", "rbs_motif": null, "genes": [ "plsC" ], "truncated": "5-prime", "pseudogene": { "start": 251781, "stop": 252587, "observations": { "mutation": [ 251973 ], "stop": [ 251973 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49402", "query_cov": 1.0, "subject_cov": 0.7276119402985075, "identity": 0.995, "score": 369.0, "evalue": 1.01e-127, "gene_start": 1, "gene_end": 195, "reference_start": 74, "reference_end": 268, "reference_sequence": "MDKLFKTSFRFIIRFLQILSLPVVFPYFLLSFLACLITSKNYESLPYNYPPEIRFKKVYRLVSMWLYIKGIKVVTVNDKIIPKKPVLVVANHKSNLDPLVLIKAFGRLKNSPPLTFVAKIELKDTVLFKLMKLIDCVFIDRKNIRQIANALETQQQLIRQGTAIAVFAEGTRILSNDIGEFKPGALKVAYNAFVPILPVSIVGSLGKMESNKRLKEHGVKKSSNYEVKVIFNKLINPISFNQIDSNNLANNIRSIISDAYTSEKPSND" }, "paralog": false, "identity": 0.996268656716418, "score": 518.0, "evalue": 2.7e-189, "description": "Internal stop codon at 251973. Nonsense mutation around 251973.", "psc": { "uniref90_id": "UniRef90_Q49402", "gene": "plsC", "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase", "ec_ids": [ "2.3.1.51" ], "uniref50_id": "UniRef50_Q49402", "cog_id": "COG0204", "cog_category": "I", "go_ids": [ "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49402", "EC:2.3.1.51", "UniRef:UniRef50_Q49402", "COG:COG0204", "COG:I", "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ] }, "pscc": { "uniref50_id": "UniRef50_Q49402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49402" ], "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "KKNAAMEMLJ_480", "locus": "GCA000027325_00445" }, { "type": "cds", "sequence": "contig_1", "start": 252754, "stop": 252942, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAACCGAATTAACCCCAAATTCAAAACTCACAGTTTTTAACAACTACCAGCAACTTTTAGTTGATAATAATGACCTAATTATCTTTGCAACCCCCACTTTAGTTCAGCTTTTTGATAATGAGATTGACCAGTTAATAGTTATCAATCCCACTAGTAAATCTAAAGATCAGTTTAACTGTAATTGA", "aa": "MQTELTPNSKLTVFNNYQQLLVDNNDLIIFATPTLVQLFDNEIDQLIVINPTSKSKDQFNCN", "aa_hexdigest": "ada697e9a5f1dabb980c0eb2747ea4bf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7115.954700000001, "isoelectric_point": 4.089929389953612 }, "id": "KKNAAMEMLJ_481", "locus": "GCA000027325_00446" }, { "type": "cds", "sequence": "contig_1", "start": 253111, "stop": 253995, "strand": "+", "frame": 1, "gene": null, "product": "segregation/condensation protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "nt": "GTGGATATTGGCTTGATTTCCTTACAATATCTCAATATCATTGCAAATTACACCAATAAAAAAGCCATTGAAAAGATCACTGATTATCTGGTGATTACAAGCAAAATTCTCGCTAAAAAAGCAGATAATCTGCTCAATGATCATCAGGAAGAGAGTGTGTTGGAATATGATTTAGCTACTAATAACTTTCGTGATAAGATGATCGCTAACTTAGTGGAACACAAACGCTATTGTGATTCACTGGGTGAATTTGAAAAACTCAGGGTTAACCGCTTAGCTTACTTTTCCAAAGCTAATGAGATGGAGCAATTTATCAAAACAGCTAATGATCAACTTGTTACAGTTGAAGATCAACTCCCTAACTATATTAGTGTCTTAAAACTCTTTCATGCTATGAACAAGTTACTGGAGATGAGACTCAGCTCTCTTCTAACAAATAAAAACATCACCATTAAAGAGTTATCAGTTGAACAGGTTCAAAAGGAGTTGGTTTTAGCAATCAAACAGTTTAACTACCAAACAGTTTCTTTAAAGCGGGTGTTATTAAAACTTAACCATCCTATCTCTTTAATGTATTTTGTTACTGCTTTTGTGGCACTTTTAGTGCTATTAAACAACCAGGTAATAGGTTTGGAACAAAAAGATTATCACAGTGAACTTTACATCTTTTTACTAGATGAAAACCAACTGAAAACCTTCCAAGAATCACCAGATGAAATGGTAAAAAGAATCCAAGCTCAACAACAGCAAAACGAACTGATAATTGCTAAAAACAAGCAACTAAGAGCTATCAAAAACAAACAAAAGCGAGCTGATTATCTAAAAAAGAAATATGGTGAAAATTACTTAGATAAAACTAACTTAAAAGATGAAAACAACAATTAA", "aa": "MDIGLISLQYLNIIANYTNKKAIEKITDYLVITSKILAKKADNLLNDHQEESVLEYDLATNNFRDKMIANLVEHKRYCDSLGEFEKLRVNRLAYFSKANEMEQFIKTANDQLVTVEDQLPNYISVLKLFHAMNKLLEMRLSSLLTNKNITIKELSVEQVQKELVLAIKQFNYQTVSLKRVLLKLNHPISLMYFVTAFVALLVLLNNQVIGLEQKDYHSELYIFLLDENQLKTFQESPDEMVKRIQAQQQQNELIIAKNKQLRAIKNKQKRADYLKKKYGENYLDKTNLKDENNN", "aa_hexdigest": "15fb8a2ef922a4dc4c61d5687f1bb74c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "query_cov": 0.9727891156462585, "subject_cov": 0.8614457831325302, "identity": 0.552, "score": 279.0, "evalue": 6.7e-90, "valid": false, "gene": "scpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_UPI000AF5CFDF", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_UPI000AF5CFDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "product": "segregation/condensation protein A" }, "genes": [], "id": "KKNAAMEMLJ_482", "locus": "GCA000027325_00447" }, { "type": "cds", "sequence": "contig_1", "start": 253979, "stop": 254602, "strand": "+", "frame": 2, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304", "KEGG:K06024", "RefSeq:WP_009885754.1", "SO:0001217", "UniParc:UPI0000139487", "UniRef:UniRef100_P47456", "UniRef:UniRef50_P75477", "UniRef:UniRef90_P47456" ], "nt": "ATGAAAACAACAATTAATATTGCCACCCCTACCCTAAAAAAACCTAGCAAAGAAGCTAACTTGGTTGCTAGTATCTATGGGTTGTTATTTGTTTGTGGCGCGAAAGGGATCACTTTAAGAGAACTAATTAGGATCTTTAAAAAAGCAGGGATTGAAAAGGTGAAATTAGCACTCTTAGCACTTGAAAGGAAGTTAGCAGATGATGAGCAATCAGGAGTGGAGTTGAAAAAATTTGGTAATAGTTTTTCTTTGGTAACAAAACCAATTATCAAAGACTATCTCCACTTATTATTGGCTCATAAAGTCAAAAATCCCCTTAATTCCAAAGCAATGGAAGTGTTGGCTATCATTGCTTACAACCAACCTTGCACCAGACCCAGAATTAATGAAATTAGGGGAGTTGATTCTTTTCAAATTGTTGATGATCTAATAGCAAAAGAGTTAATTGTGGAGTTAGGGAGAACTGATAAACCAGGTCGACCTTTTATTTATGAAGTGTCAGCTAAGTTCTATGATTTATTTGGCATTGATAGCTTAGATCAACTCCCTAAGATTGAGCATTTTGATCTTGATAAATTTAAGCAAGGTAGCTTTTTTGATTCCAACCGCTATGGTGATGAATAA", "aa": "MKTTINIATPTLKKPSKEANLVASIYGLLFVCGAKGITLRELIRIFKKAGIEKVKLALLALERKLADDEQSGVELKKFGNSFSLVTKPIIKDYLHLLLAHKVKNPLNSKAMEVLAIIAYNQPCTRPRINEIRGVDSFQIVDDLIAKELIVELGRTDKPGRPFIYEVSAKFYDLFGIDSLDQLPKIEHFDLDKFKQGSFFDSNRYGDE", "aa_hexdigest": "1dd1f4609d2ad8e124f79d2eb5f29b0d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139487", "ncbi_nrp_id": "WP_009885754.1", "uniref100_id": "UniRef100_P47456", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139487", "RefSeq:WP_009885754.1", "UniRef:UniRef100_P47456" ] }, "ips": { "uniref100_id": "UniRef100_P47456", "uniref90_id": "UniRef90_P47456" }, "psc": { "uniref90_id": "UniRef90_P47456", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_P75477", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304" ] }, "pscc": { "uniref50_id": "UniRef50_P75477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75477" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "KKNAAMEMLJ_483", "locus": "GCA000027325_00448" }, { "type": "cds", "sequence": "contig_1", "start": 254621, "stop": 255040, "strand": "+", "frame": 2, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005737", "GO:0006002", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75476", "UniRef:UniRef90_P47457" ], "nt": "ATGGATAAAATAGCTATTTTAACTTCGGGTGGTGATGCTAGTGGGATGAATGCCACCATCGCTTATCTAACCAAATATGCAATTGCAAAGCAATTGGAAGTTTTTTATGTAAAAAACGGTTATTATGGCTTGTATCACAACCATTTTATCACCAGTAAGGAACTTGATTTAACTGACTTTTTCTTTATGGGGGGAACAGTAATAGGATCAAGTCGTTTCAAACAGTTTCAAGATCCTAGCTTACGAAAACAAGCAGTTTTAAACCTCAAAAAACGTGGTATTAACAACCTTGTTGTTATTGGTGGGGATGGGAGTTATATGGGTGCTAAAGCACTCAGTGAATTAGGATTAAACTGCTTTTGTTTACCTGGTACGATCGACAATGATGTCAATTCCAGTGAATTTACCATTGGTTTTTGA", "aa": "MDKIAILTSGGDASGMNATIAYLTKYAIAKQLEVFYVKNGYYGLYHNHFITSKELDLTDFFFMGGTVIGSSRFKQFQDPSLRKQAVLNLKKRGINNLVVIGGDGSYMGAKALSELGLNCFCLPGTIDNDVNSSEFTIGF", "aa_hexdigest": "7d4c7a73cc710480e6da8e0a33dd1a92", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X1PN14", "query_cov": 0.9928057553956835, "subject_cov": 0.8846153846153846, "identity": 0.514, "score": 135.0, "evalue": 4e-38, "valid": false, "product": "6-phosphofructokinase", "uniref50_id": "UniRef50_A0A2X1PN14" }, "genes": [ "pfkA" ], "truncated": "3-prime", "pseudogene": { "start": 254621, "stop": 255343, "observations": { "mutation": [ 255038 ], "stop": [ 255038 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47457", "query_cov": 1.0, "subject_cov": 0.43034055727554177, "identity": 1.0, "score": 279.0, "evalue": 1.27e-92, "gene_start": 1, "gene_end": 139, "reference_start": 1, "reference_end": 139, "reference_sequence": "MDKIAILTSGGDASGMNATIAYLTKYAIAKQLEVFYVKNGYYGLYHNHFITSKELDLTDFFFMGGTVIGSSRFKQFQDPSLRKQAVLNLKKRGINNLVVIGGDGSYMGAKALSELGLNCFCLPGTIDNDVNSSEFTIGFWTALEAIRVNVEAIYHTTKSHNRLAIIEVMGRDCSDLTIFGGLATNASFVVTSKNSLDLNGFEKAVRKVLQFQNYCVVLVSENIYGKNGLPSLEMVKEHFENNAIKCNLVSLGHTQRGFSPNSIELFQISLMAKHTIDLVVNNANSQVIGMKNNQAVNYDFNTAFNLPKADRTKLLNQVNTAII" }, "paralog": false, "identity": 0.9958333333333333, "score": 475.0, "evalue": 3.65e-172, "description": "Internal stop codon at 255038. Nonsense mutation around 255038.", "psc": { "uniref90_id": "UniRef90_P47457", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_P75476", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47457", "EC:2.7.1.11", "UniRef:UniRef50_P75476", "COG:COG0205", "COG:G", "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_P75476", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75476" ], "product": "Probable ATP-dependent 6-phosphofructokinase" } }, "id": "KKNAAMEMLJ_484", "locus": "GCA000027325_00449" }, { "type": "cds", "sequence": "contig_1", "start": 255125, "stop": 255592, "strand": "+", "frame": 2, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005737", "GO:0006002", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75476", "UniRef:UniRef90_P47457" ], "nt": "ATGGGGCGTGATTGTAGTGATCTGACCATCTTTGGGGGGTTAGCTACTAATGCTAGTTTTGTTGTTACTAGCAAAAATAGCTTGGATCTCAATGGCTTTGAAAAAGCAGTGAGAAAGGTGTTGCAATTCCAGAACTATTGTGTTGTTTTGGTTAGTGAAAACATCTATGGTAAGAACGGTTTACCTAGTTTAGAAATGGTTAAAGAGCACTTTGAAAACAACGCAATTAAGTGTAACCTAGTTTCACTAGGACACACCCAAAGGGGCTTTAGTCCTAATAGTATCGAACTCTTTCAGATTAGTTTAATGGCTAAACACACGATTGATCTGGTTGTAAATAATGCCAACAGTCAAGTAATAGGGATGAAAAACAACCAAGCAGTTAACTATGATTTTAACACTGCTTTTAATTTACCAAAAGCTGATAGAACCAAGTTACTTAACCAAGTTAACACTGCAATTATTTAA", "aa": "MGRDCSDLTIFGGLATNASFVVTSKNSLDLNGFEKAVRKVLQFQNYCVVLVSENIYGKNGLPSLEMVKEHFENNAIKCNLVSLGHTQRGFSPNSIELFQISLMAKHTIDLVVNNANSQVIGMKNNQAVNYDFNTAFNLPKADRTKLLNQVNTAII", "aa_hexdigest": "36160e26e6bbf3b864ec88eb0d2db9a1", "start_type": "ATG", "rbs_motif": null, "genes": [ "pfkA" ], "truncated": "5-prime", "pseudogene": { "start": 254825, "stop": 255592, "observations": { "mutation": [ 255038 ], "stop": [ 255038 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47457", "query_cov": 1.0, "subject_cov": 0.47987616099071206, "identity": 1.0, "score": 306.0, "evalue": 5.71e-103, "gene_start": 1, "gene_end": 155, "reference_start": 169, "reference_end": 323, "reference_sequence": "MDKIAILTSGGDASGMNATIAYLTKYAIAKQLEVFYVKNGYYGLYHNHFITSKELDLTDFFFMGGTVIGSSRFKQFQDPSLRKQAVLNLKKRGINNLVVIGGDGSYMGAKALSELGLNCFCLPGTIDNDVNSSEFTIGFWTALEAIRVNVEAIYHTTKSHNRLAIIEVMGRDCSDLTIFGGLATNASFVVTSKNSLDLNGFEKAVRKVLQFQNYCVVLVSENIYGKNGLPSLEMVKEHFENNAIKCNLVSLGHTQRGFSPNSIELFQISLMAKHTIDLVVNNANSQVIGMKNNQAVNYDFNTAFNLPKADRTKLLNQVNTAII" }, "paralog": false, "identity": 0.996078431372549, "score": 501.0, "evalue": 2.15e-182, "description": "Internal stop codon at 255038. Nonsense mutation around 255038.", "psc": { "uniref90_id": "UniRef90_P47457", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_P75476", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47457", "EC:2.7.1.11", "UniRef:UniRef50_P75476", "COG:COG0205", "COG:G", "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_P75476", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75476" ], "product": "Probable ATP-dependent 6-phosphofructokinase" } }, "id": "KKNAAMEMLJ_485", "locus": "GCA000027325_00450" }, { "type": "cds", "sequence": "contig_1", "start": 255595, "stop": 256458, "strand": "+", "frame": 1, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006096", "GO:0016301", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_P78031", "UniRef:UniRef90_P47458" ], "nt": "ATGATTGACCATTTAAAAAGAACAAAGATAATCGCTACCTGTGGCCCAGCTTTAACAAAAAGCTTGGTTAGCTTAAAGATGCTTGATGATAATGAGTATGCAGCTATTAAAAAGGTTGCTTATGCCAACATTGAAGCAATTATTAAAAGTGGGGTTAGTGTGATTAGGCTTAACTTCTCTCATGGTACCCATGAAGAACAACAAGTGAGGATCAAGATAGTAAGGGATGTAGCGAAAGCAATGAACATCCCTGTTTCTATTATGTTAGATACAAATGGTCCTGAGATCAGGATAGTAGAAACTAAAAAAGAGGGTTTGAAAATCACCAAAGATAGTGAAGTGATTATCAACACCATGAGTAAAATGATCGCTAGTGACAACCAGTTTGCTGTCAGTGATGCTAGTGGCAAATACAACATGGTTAATGATGTGAATATAGGTCAGAAAATCCTTGTTGATGATGGTAAGTTAACCCTGGTTGTCACAAGGGTTGACAAACAACATAACCAGGTTATCTGTGTTGCAAAAAACGACCACACAGTTTTCACTAAAAAAAGACTTAACCTACCCAACGCACAGTACTCTATCCCTTTTCTCAGTGAAAAGGATCTGAAGGATATTGACTTTGGTTTAAGCCAAGGTATTGACTATATTGCTGCCTCTTTTGTTAATACTGTTGCAGATATTAAACAACTGAGAGATTATCTGAAATTAAAGAATGCTAGTGGGGTGAAGATCATCGCTAAGATTGAATCTAATCATGCTTTAAATAACATTGATAAGATCATTAAAGCTAGCGATGGGATTATGGTTGCTAGGGGTGATTTGGGCCTTGAAATCCCTTATTACCAAGTCCCTTACTGA", "aa": "MIDHLKRTKIIATCGPALTKSLVSLKMLDDNEYAAIKKVAYANIEAIIKSGVSVIRLNFSHGTHEEQQVRIKIVRDVAKAMNIPVSIMLDTNGPEIRIVETKKEGLKITKDSEVIINTMSKMIASDNQFAVSDASGKYNMVNDVNIGQKILVDDGKLTLVVTRVDKQHNQVICVAKNDHTVFTKKRLNLPNAQYSIPFLSEKDLKDIDFGLSQGIDYIAASFVNTVADIKQLRDYLKLKNASGVKIIAKIESNHALNNIDKIIKASDGIMVARGDLGLEIPYYQVPY", "aa_hexdigest": "792210993a97586d312b7cc544bc540e", "start_type": "ATG", "rbs_motif": null, "genes": [ "pyk" ], "truncated": "3-prime", "pseudogene": { "start": 255595, "stop": 256761, "observations": { "mutation": [ 256456 ], "stop": [ 256456 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47458", "query_cov": 1.0, "subject_cov": 0.5649606299212598, "identity": 1.0, "score": 556.0, "evalue": 1.49e-196, "gene_start": 1, "gene_end": 287, "reference_start": 1, "reference_end": 287, "reference_sequence": "MIDHLKRTKIIATCGPALTKSLVSLKMLDDNEYAAIKKVAYANIEAIIKSGVSVIRLNFSHGTHEEQQVRIKIVRDVAKAMNIPVSIMLDTNGPEIRIVETKKEGLKITKDSEVIINTMSKMIASDNQFAVSDASGKYNMVNDVNIGQKILVDDGKLTLVVTRVDKQHNQVICVAKNDHTVFTKKRLNLPNAQYSIPFLSEKDLKDIDFGLSQGIDYIAASFVNTVADIKQLRDYLKLKNASGVKIIAKIESNHALNNIDKIIKASDGIMVARGDLGLEIPYYQVPYWQRYMIKACRFFNKRSITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETASGLYPLNAVAVMQKIDKQSETFFDYQYNVNYYLKNSTANKSRFWHNVVLPLTKKTVPKRKLVNSAFKYDFIVYPTNNINRIYALSNARLAAAVIILTNNKRVYTGHGVDYGIFCYLIDKNPNQLTKAELIELAWKAINHYQAYGDLEKLKQCLAVYNETIINL" }, "paralog": false, "identity": 0.9974226804123711, "score": 752.0, "evalue": 2.75e-276, "description": "Internal stop codon at 256456. Nonsense mutation around 256456.", "psc": { "uniref90_id": "UniRef90_P47458", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_P78031", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006096", "GO:0016301", "GO:0030955" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47458", "EC:2.7.1.40", "UniRef:UniRef50_P78031", "COG:COG0469", "COG:G", "KEGG:K00873", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006096", "GO:0016301", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_P78031", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78031" ], "product": "Pyruvate kinase" } }, "id": "KKNAAMEMLJ_486", "locus": "GCA000027325_00451" }, { "type": "cds", "sequence": "contig_1", "start": 256468, "stop": 256761, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "ATGATTAAAGCTTGTCGCTTTTTTAACAAGCGTTCTATTACTGCAACCCAAATGCTTGATTCACTAGAAAAAAACATCCAACCAACCCGAGCTGAAGTGACTGATGTTTACTTTGCAGTTGATCGGGGTAATGATGCAACTATGTTAAGTGGGGAAACTGCTAGTGGGCTTTACCCTTTAAATGCAGTAGCGGTGATGCAAAAGATTGATAAACAATCAGAAACCTTCTTTGATTACCAGTATAACGTTAACTATTATTTGAAAAACTCCACGGCAAATAAAAGTAGGTTTTGA", "aa": "MIKACRFFNKRSITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETASGLYPLNAVAVMQKIDKQSETFFDYQYNVNYYLKNSTANKSRF", "aa_hexdigest": "d15edec308c290aec65533dd551eabf1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 63, "aa_cov": 0.6494845360824743, "hmm_cov": 1.0, "evalue": 2.1987378249766233e-23, "score": 77.8996353149414, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 11096.425699999994, "isoelectric_point": 8.732000923156736 }, "id": "KKNAAMEMLJ_487", "locus": "GCA000027325_00452" }, { "type": "tRNA", "sequence": "contig_1", "start": 257158, "stop": 257234, "strand": "+", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 60.0, "nt": "GGTGCCTTAGCCAAGTGGACTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 257193, 257195 ], "id": "KKNAAMEMLJ_1", "locus": "GCA000027325_00453" }, { "type": "tRNA", "sequence": "contig_1", "start": 257269, "stop": 257345, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 83.1, "nt": "CGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 257286, 257288 ], "id": "KKNAAMEMLJ_2", "locus": "GCA000027325_00454" }, { "type": "tRNA", "sequence": "contig_1", "start": 257349, "stop": 257425, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 81.0, "nt": "GGCTGGATACCTCAGTTGGTTAGAGGGCCCGGTTCATACCCGGGTTGTCGTGAGTTCGAGTCTCACTCCAGCCACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 257383, 257385 ], "id": "KKNAAMEMLJ_3", "locus": "GCA000027325_00455" }, { "type": "tRNA", "sequence": "contig_1", "start": 257445, "stop": 257521, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 74.3, "nt": "GGATCTATAGCTCAATTGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCAAGTCCTGTTAGATCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "KKNAAMEMLJ_4", "locus": "GCA000027325_00456" }, { "type": "tRNA", "sequence": "contig_1", "start": 257559, "stop": 257650, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 76.9, "nt": "GGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTTTATAAGCACGCGAGGGTTCGAATCCCTCAGTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 257578, 257580 ], "id": "KKNAAMEMLJ_5", "locus": "GCA000027325_00457" }, { "type": "tRNA", "sequence": "contig_1", "start": 257664, "stop": 257740, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 78.9, "nt": "CGCGGGATAGAGCAGTTGGTCAGCTCGTCAGGCTCATAATCTGAAGGTCGAGGGTTCAAATCCCTCTCCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "KKNAAMEMLJ_6", "locus": "GCA000027325_00458" }, { "type": "tRNA", "sequence": "contig_1", "start": 257742, "stop": 257815, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 55.6, "nt": "GGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 257774, 257776 ], "id": "KKNAAMEMLJ_7", "locus": "GCA000027325_00459" }, { "type": "tRNA", "sequence": "contig_1", "start": 257818, "stop": 257893, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 77.8, "nt": "GGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 257851, 257853 ], "id": "KKNAAMEMLJ_8", "locus": "GCA000027325_00460" }, { "type": "cds", "sequence": "contig_1", "start": 258041, "stop": 259159, "strand": "+", "frame": 2, "gene": null, "product": "cytadherence-associated protein P65", "db_xrefs": [ "BlastRules:WP_010869376", "BlastRules:WP_010874665", "RefSeq:WP_010869376.1", "SO:0001217", "UniParc:UPI0000131062", "UniRef:UniRef100_P47459", "UniRef:UniRef50_P47459", "UniRef:UniRef90_P47459" ], "nt": "ATGGAAAAAAATAGATCAGCTTTTCAACAAAACCAACAAGCATCAAACCAACCTTTTAACCAAGATCAAAACCAGTATTACCAAGATCCTAACCAACAACAATTTAACCAATCTGGTTTTGATCCAAATCAACAGCAATTTAATCAACCAGGATTTGATCCTAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAAGCTGGTTTTGATCAAAACCAACAGTATTACCAAGATCCCAATCAACAGCAATTTAATCAACCAGGATTTGATCCTAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAAGCTGGTTTTGATCAAAACCAGTATTACCAAGATCCTAACCAACAACAATTTAACCAATCTGGTTTTGATCAAAACCAGTACTATCAAGATCCTAATCAGCAACAATTTAACCAGCCTAGTTTTGATCTAAATAACCAACAATTTAACCAACCTGGATTCAACCAATCCCCAGCATTTGAAATCACACCTCAAGAGCAAAAAGCTGAACAGGAAATGTTTGGTGAAGAACCACCTCAAGTAGTTAGAGAGATCCATGAACTACCATTTGAAAAGATCCGTTCTTTTTTACAAAGTGATTTTGATAGCTATAACTTTCGCTTAAACTCACTTAAAAGCAAACTGGATAATGCCTTGTATTCACTTGATAAAACAATCCAAAACACCAATGAAAACACTGCTAATCTAGAAGCAATTAGACATAACTTAGAACAAAAGATTCAAAACCAAAGCAAGCAATTAAGAACTAACTTTGATACCCAAAAGCTTGATGATAAGATCAATGAATTGGAGATCAGAATGCAAAAACTAACCAGGAATTTTGAATCTCTAAGTGAACTTTCAAAGCACAACTCTTATCCTAATTACTATGAAAAATTGTTACCAAATGGTGGTGATAGTATGACCAATGTCTTTGAAAAAGCACTAATGATGAATTTATTGAGAACTACATTACCCCCTCAACCCCAAGTTCAATACTACCCTCAACCCTATCCATACATAAGACCTTACTATGATGAACCTATTTACGCTGGGTTTAGAAGAAGGGGTTACCGTGATGACTTCTATGAATAA", "aa": "MEKNRSAFQQNQQASNQPFNQDQNQYYQDPNQQQFNQSGFDPNQQQFNQPGFDPNQQYYQDPNQQQFNQAGFDQNQQYYQDPNQQQFNQPGFDPNQQYYQDPNQQQFNQAGFDQNQYYQDPNQQQFNQSGFDQNQYYQDPNQQQFNQPSFDLNNQQFNQPGFNQSPAFEITPQEQKAEQEMFGEEPPQVVREIHELPFEKIRSFLQSDFDSYNFRLNSLKSKLDNALYSLDKTIQNTNENTANLEAIRHNLEQKIQNQSKQLRTNFDTQKLDDKINELEIRMQKLTRNFESLSELSKHNSYPNYYEKLLPNGGDSMTNVFEKALMMNLLRTTLPPQPQVQYYPQPYPYIRPYYDEPIYAGFRRRGYRDDFYE", "aa_hexdigest": "ab2a50e714eb873baddf5847e6d33415", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000131062", "ncbi_nrp_id": "WP_010869376.1", "uniref100_id": "UniRef100_P47459", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000131062", "RefSeq:WP_010869376.1", "UniRef:UniRef100_P47459" ] }, "ips": { "uniref100_id": "UniRef100_P47459", "uniref90_id": "UniRef90_P47459" }, "psc": { "uniref90_id": "UniRef90_P47459", "product": "Proline-rich P65 protein homolog", "uniref50_id": "UniRef50_P47459" }, "pscc": { "uniref50_id": "UniRef50_P47459", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47459" ], "product": "Proline-rich P65 protein homolog" }, "expert": [ { "type": "expert_proteins", "source": "BlastRules", "rank": 70, "id": "WP_010869376", "gene": null, "product": "cytadherence-associated protein P65", "query_cov": 1.0, "subject_cov": 1.0, "identity": 1.0, "score": 445.0, "evalue": 5.01e-157, "db_xrefs": [ "BlastRules:WP_010869376", "BlastRules:WP_010874665" ] } ], "genes": [], "id": "KKNAAMEMLJ_488", "locus": "GCA000027325_00461" }, { "type": "cds", "sequence": "contig_1", "start": 259098, "stop": 259190, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAACCTATTTACGCTGGGTTTAGAAGAAGGGGTTACCGTGATGACTTCTATGAATAAAAAGCGTGTTTTAACTAATGAAACCATTTGA", "aa": "MMNLFTLGLEEGVTVMTSMNKKRVLTNETI", "aa_hexdigest": "c7d305b1308506acc292e516438af4c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3402.0336000000007, "isoelectric_point": 5.986078071594237 }, "id": "KKNAAMEMLJ_489", "locus": "GCA000027325_00462" }, { "type": "cds", "sequence": "contig_1", "start": 259177, "stop": 262329, "strand": "+", "frame": 1, "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0020035", "SO:0001217", "UniRef:UniRef50_P75471", "UniRef:UniRef90_P47460" ], "nt": "ATGAAACCATTTGATAAAAAACCTTCGCTGCAACCAATTTATGACATTGGTTTTGATGATGGTTATCTCCAAAGTGAGTATGAAAAAAATCGTTCTAAAACCGATGTTGATAAGATCGAAAACCAGCTTTTAAAAGAGATTAAAAGCCTGGAAGATGAACTTAAAAACCTTAAGGGCTTGAAGAATCAAGCAGAAGATAATCCTGAACTTGATAAAAAGATTAACCACTTGGAAGTTGATCTAAACCGTTTGGTTAATGAATATAAAAACTTCCAGTTCCAAAAGAACCACATGGTTGATAAGGTTAGTGAACTTGATAACTTAACCCGTTTTTATAAGAATGAACTAACCCGCTTACAACAAGAAAACGCTGATTTTCTCAACTCCAAGTATGCTAATTTAGCTAACTTCCAAGCTAACTACCACAATAAACTAAATGATTTTCACCGCTTAATAGAAAATCAAAACCAAACCATTAACCGCTTAAACCAAAAGATTAATGGTAACCAAAATCTGATTGATAATAACGTTGCTTTACTGCAAAACCCCAACATCACAGTTGAAAAAAAGAACTACTTACTAAATGTTATTGATCAACTTTACAATGAGCTTGATCAACTTGAGAATCAAAAAAGATTATTAAGTATTGAGTATGAAAATACCTATAGAGAGTTAGTTAGTGCAGATAATGAACTGCAAAATGTTTATGAAAACATCGATCAAAATCAGATCCAGTTTAAACACCAATACCAAACTTATAGAGATGAGTTAAGTCAACTTGAGCGCAAGATCCAGCTCACCAAACAAGAGTTAGTTGATAAAGAATCAGCACTAAGAGTAAAGATAGATGATGCTGATTTTTACATTAATGCCCGTTTAGCTGAACTTGATGATGTAGCTAAACAACTTAGCTTTCAAGATGGTATTACCAAGCAAAATGCTCAACATGTTGAGGATAAGTTAGTTGCTTTAAACAAAGAAAAAGACCGTTTAAATACCCAAAAAGAGGCCTTTTTTAACTTAAGACAATCTGCTTTAATTGATATCAATAAACTCCAGCAGGAAAATGAACTGTTTGCTAAGCACTTAGAACACCAGCAAAATGAGTTTGAACAAAAACAGTCTGATAGCCTCTTAAAGCTTGAAACTGAATATAAGGCTTTACAACACAAGATTAATGAATTTAAAAATGAAAGTGCCACTAAGAGCGAGGAACTTTTAAACCAAGAACGGGAACTATTTGAAAAACGCAGGGAAATTGACACGCTTTTAACCCAAGCATCTTTGGAATATGAACACCAACGTGAGTCAAGTCAACTACTCAAAGATAAGCAGAATGAAGTAAAACAACACTTCCAAAACTTAGAGTATGCTAAAAAGGAGCTAGACAAAGAAAGAAACCTCTTAGATCAACAAAAAAAAGTTGATAGTGAAGCAATCTTTCAACTCAAAGAAAAGGTTGCTCAAGAACGTAAGGAGCTTGAAGAACTATACCTTGTTAAAAAACAAAAACAAGATCAGAAGGAAAATGAACTGTTGTTTTTTGAAAAGCAGTTAAAACAACACCAAGCAGATTTTGAAAATGAATTGGAAGCTAAACAACAGGAGTTGTTTGAAGCTAAACATGCATTGGAACGTTCCTTTATCAAACTTGAAGATAAAGAAAAAGATCTTAACACTAAAGCACAACAGATTGCCAATGAGTTTTCCCAACTAAAAACTGATAAGTCAAAGAGTGCTGATTTTGAACTAATGTTGCAAAATGAGTATGAAAACTTGCAACAAGAAAAACAAAAGTTATTCCAAGAACGTACTTACTTTGAAAGGAATGCTGCGGTTTTATCAAACCGGTTACAACAAAAACGTGAGGAGTTATTACAACAAAAAGAAACGCTTGATCAGCTTACAAAAAGCTTTGAGCAAGAACGGTTAATCAACCAAAGGGAACACAAGGAGTTGGTTGCATCAGTTGAAAAACAAAAGGAGATATTGGGCAAAAAACTCCAAGATTTTTCCCAAACTTCACTGAACGCTTCTAAAAATTTAGCTGAACGGGAGATGGCAATCAAGTTTAAAGAAAAGGAGATAGAAGCAACTGAAAAGCAACTGTTAAATGATGTTAATAATGCTGAAGTTATCCAAGCAGACTTAGCACAACTCAACCAATCACTTAACCAAGAACGCAGTGAATTGCAAAACGCCAAACAAAGGATTGCTGATTTTCACAATGATTCACTAAAAAAACTCAATGAGTATGAACTTAGCTTACAAAAACGGTTGCAAGAATTACAAACCCTTGAGGCTAACCAAAAACAACATTCATATCAAAATCAAGCTTACTTTGAAGGTGAACTTGATAAACTTAACAGAGAAAAACAAGCTTTTTTGAACTTACGTAAGAAACAAACTATGGAGGTTGATGCTATTAAACAAAGGTTGAGTGATAAACATCAAGCTTTAAATATGCAACAAGCAGAGCTAGATAGAAAAACCCATGAGTTAAATAATGCTTTTTTAAACCATGATGCGGATCAAAAGAGTCTACAGGACCAACTAGCAACTGTTAAAGAGACCCAAAAACTAATTGATTTAGAACGTAGTGCACTGCTTGAAAAGCAACGTGAGTTTGCTGAAAATGTTGCTGGTTTTAAGCGCCATTGGTCTAATAAAACTAGTCAACTCCAAAAGATTTATGAACTGACCAAAAAACAGGAAAGTGAGCAAACCCAAAAGGAAACAGAACTAAAGATTGCTTTTAGTGATCTACAAAAAGACTATCAGGTTTTTGAACTCCAAAAGGACCAAGAATTTAGACAAATTGAAGCTAAGCAACGTGAACTTGACAAGTTAGCTGAAAAAAATAATCAGGTCAAACTAGAACTTGATAACAGGTTTCAAGCGCTGCAAAACCAAAAGCAAGACACAGTACAAGCTCAGCTAGAACTGGAACGTGAACAACACCAGTTAAACCTTGAGCAAACTGCTTTCAACCAAGCTAATGAATCACTTTTAAAACAACGTGAACAACTCACCAAAAAGATCCAAGCTTTCCACTATGAGTTGAAAAAGCGTAACCAATTCTTAGCTTTAAAAGGGAAAAGGTTGTTTGCAAAAGAGCAAGATCAACAACGCAAAGATCAGGAGATCAACTGA", "aa": "MKPFDKKPSLQPIYDIGFDDGYLQSEYEKNRSKTDVDKIENQLLKEIKSLEDELKNLKGLKNQAEDNPELDKKINHLEVDLNRLVNEYKNFQFQKNHMVDKVSELDNLTRFYKNELTRLQQENADFLNSKYANLANFQANYHNKLNDFHRLIENQNQTINRLNQKINGNQNLIDNNVALLQNPNITVEKKNYLLNVIDQLYNELDQLENQKRLLSIEYENTYRELVSADNELQNVYENIDQNQIQFKHQYQTYRDELSQLERKIQLTKQELVDKESALRVKIDDADFYINARLAELDDVAKQLSFQDGITKQNAQHVEDKLVALNKEKDRLNTQKEAFFNLRQSALIDINKLQQENELFAKHLEHQQNEFEQKQSDSLLKLETEYKALQHKINEFKNESATKSEELLNQERELFEKRREIDTLLTQASLEYEHQRESSQLLKDKQNEVKQHFQNLEYAKKELDKERNLLDQQKKVDSEAIFQLKEKVAQERKELEELYLVKKQKQDQKENELLFFEKQLKQHQADFENELEAKQQELFEAKHALERSFIKLEDKEKDLNTKAQQIANEFSQLKTDKSKSADFELMLQNEYENLQQEKQKLFQERTYFERNAAVLSNRLQQKREELLQQKETLDQLTKSFEQERLINQREHKELVASVEKQKEILGKKLQDFSQTSLNASKNLAEREMAIKFKEKEIEATEKQLLNDVNNAEVIQADLAQLNQSLNQERSELQNAKQRIADFHNDSLKKLNEYELSLQKRLQELQTLEANQKQHSYQNQAYFEGELDKLNREKQAFLNLRKKQTMEVDAIKQRLSDKHQALNMQQAELDRKTHELNNAFLNHDADQKSLQDQLATVKETQKLIDLERSALLEKQREFAENVAGFKRHWSNKTSQLQKIYELTKKQESEQTQKETELKIAFSDLQKDYQVFELQKDQEFRQIEAKQRELDKLAEKNNQVKLELDNRFQALQNQKQDTVQAQLELEREQHQLNLEQTAFNQANESLLKQREQLTKKIQAFHYELKKRNQFLALKGKRLFAKEQDQQRKDQEIN", "aa_hexdigest": "822b6c008de8ee61aa65ea37b1ae4e8f", "start_type": "ATG", "rbs_motif": null, "genes": [ "hmw2" ], "truncated": "3-prime", "pseudogene": { "start": 259177, "stop": 262632, "observations": { "mutation": [ 262327 ], "stop": [ 262327 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47460", "query_cov": 1.0, "subject_cov": 0.5817174515235457, "identity": 1.0, "score": 1575.0, "evalue": 0.0, "gene_start": 1, "gene_end": 1050, "reference_start": 1, "reference_end": 1050, "reference_sequence": "MKPFDKKPSLQPIYDIGFDDGYLQSEYEKNRSKTDVDKIENQLLKEIKSLEDELKNLKGLKNQAEDNPELDKKINHLEVDLNRLVNEYKNFQFQKNHMVDKVSELDNLTRFYKNELTRLQQENADFLNSKYANLANFQANYHNKLNDFHRLIENQNQTINRLNQKINGNQNLIDNNVALLQNPNITVEKKNYLLNVIDQLYNELDQLENQKRLLSIEYENTYRELVSADNELQNVYENIDQNQIQFKHQYQTYRDELSQLERKIQLTKQELVDKESALRVKIDDADFYINARLAELDDVAKQLSFQDGITKQNAQHVEDKLVALNKEKDRLNTQKEAFFNLRQSALIDINKLQQENELFAKHLEHQQNEFEQKQSDSLLKLETEYKALQHKINEFKNESATKSEELLNQERELFEKRREIDTLLTQASLEYEHQRESSQLLKDKQNEVKQHFQNLEYAKKELDKERNLLDQQKKVDSEAIFQLKEKVAQERKELEELYLVKKQKQDQKENELLFFEKQLKQHQADFENELEAKQQELFEAKHALERSFIKLEDKEKDLNTKAQQIANEFSQLKTDKSKSADFELMLQNEYENLQQEKQKLFQERTYFERNAAVLSNRLQQKREELLQQKETLDQLTKSFEQERLINQREHKELVASVEKQKEILGKKLQDFSQTSLNASKNLAEREMAIKFKEKEIEATEKQLLNDVNNAEVIQADLAQLNQSLNQERSELQNAKQRIADFHNDSLKKLNEYELSLQKRLQELQTLEANQKQHSYQNQAYFEGELDKLNREKQAFLNLRKKQTMEVDAIKQRLSDKHQALNMQQAELDRKTHELNNAFLNHDADQKSLQDQLATVKETQKLIDLERSALLEKQREFAENVAGFKRHWSNKTSQLQKIYELTKKQESEQTQKETELKIAFSDLQKDYQVFELQKDQEFRQIEAKQRELDKLAEKNNQVKLELDNRFQALQNQKQDTVQAQLELEREQHQLNLEQTAFNQANESLLKQREQLTKKIQAFHYELKKRNQFLALKGKRLFAKEQDQQRKDQEINWRFKQFEKEYTDFDEAKKRELEELEKIRRSLSQSNVELERKREKLATDFTNLNKVQHNTQINRDQLNSQIRQFLLERKNFQRFSNEANAKKAFLIKRLRSFASNLKLQKEALAIQKLEFDKRDEQQKKELQQATLQLEQFKFEKQNFDIEKQRQLVAIKTQCEKLSDEKKALNQKLVELKNLSQTYLANKNKAEYSQQQLQQKYTNLLDLKENLERTKDQLDKKHRSIFARLTKFANDLRFEKKQLLKAQRIVDDKNRLLKENERNLHFLSNETERKRAVLEDQISYFEKQRKQATDAILASHKEVKKKEGELQKLLVELETRKTKLNNDFAKFSRQREEFENQRLKLLELQKTLQTQTNSNNFKTKAIQEIENSYKRGMEELNFQKKEFDKNKSRLYEYFRKMRDEIERKESQVKLVLKETQRKANLLEAQANKLNIEKNTIDFKEKELKAFKDKVDQDIDSTNKQRKELNELLNENKLLQQSLIERERAINSKDSLLNKKIETIKRQLHDKEMRVLRLVDRMKLAEQKYQTEINRLRTQTFDSEKQDIKNFFPPLFKINGNDMAFPYLYPWLYPQQKQDDNTLQIRQLFEQQLQFMQQRYENELNELRRQRNLLEKKLDQIQLESQLNNKQSEFSKVESMMEKLLEKTESRLNDFDQKINYLTKKVNQHNTYQPSSYQPTPSYQDSDKQQLLFRIQELEKQNLFQQQFQPAPAVVQQPTSFAAPNITKQQQIAQLNAEINNIKRLIAQKAASK" }, "paralog": false, "identity": 0.9991311902693311, "score": 1906.0, "evalue": 0.0, "description": "Internal stop codon at 262327. Nonsense mutation around 262327.", "psc": { "uniref90_id": "UniRef90_P47460", "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "uniref50_id": "UniRef50_P75471", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47460", "UniRef:UniRef50_P75471", "COG:COG1196", "COG:D", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_P75471", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75471" ], "product": "Cytadherence high molecular weight protein 2" } }, "id": "KKNAAMEMLJ_490", "locus": "GCA000027325_00463" }, { "type": "cds", "sequence": "contig_1", "start": 263104, "stop": 264045, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAAATGAACGTAACCTCCATTTCCTTTCCAATGAAACAGAACGAAAACGAGCAGTTCTCGAAGATCAAATTTCTTACTTTGAAAAACAACGTAAACAAGCTACTGATGCGATCCTAGCATCACATAAAGAAGTTAAAAAGAAGGAAGGTGAACTGCAAAAGTTACTGGTTGAATTAGAAACAAGAAAAACCAAACTCAACAATGATTTTGCAAAATTCTCAAGACAACGTGAAGAGTTTGAAAACCAACGCTTAAAGCTCTTGGAACTGCAAAAAACCCTGCAAACCCAAACTAATTCCAACAACTTTAAAACCAAAGCAATCCAAGAGATTGAAAACAGTTATAAAAGGGGGATGGAAGAACTTAACTTCCAAAAGAAGGAGTTTGATAAGAATAAATCACGCTTATATGAATACTTTAGAAAGATGCGTGATGAGATTGAAAGAAAGGAAAGTCAGGTTAAGTTAGTTTTAAAAGAGACCCAAAGGAAAGCCAACCTCTTAGAAGCACAAGCCAACAAACTTAACATTGAAAAAAACACTATTGACTTTAAAGAAAAAGAGTTAAAAGCCTTTAAAGATAAGGTTGATCAAGACATTGATTCAACCAATAAACAACGCAAGGAGTTAAATGAGCTTTTAAATGAAAACAAGTTATTACAACAATCACTAATCGAAAGAGAAAGGGCTATTAATTCCAAAGATTCACTTCTAAATAAGAAGATAGAAACGATTAAACGCCAACTCCATGATAAGGAGATGCGGGTGTTGCGCTTGGTTGATAGAATGAAATTAGCAGAACAGAAATACCAAACAGAAATCAACCGCTTAAGAACCCAAACTTTCGATTCTGAAAAACAAGACATTAAAAACTTCTTCCCACCGTTATTTAAAATTAACGGTAATGATATGGCCTTTCCTTACTTGTACCCCTGA", "aa": "MKENERNLHFLSNETERKRAVLEDQISYFEKQRKQATDAILASHKEVKKKEGELQKLLVELETRKTKLNNDFAKFSRQREEFENQRLKLLELQKTLQTQTNSNNFKTKAIQEIENSYKRGMEELNFQKKEFDKNKSRLYEYFRKMRDEIERKESQVKLVLKETQRKANLLEAQANKLNIEKNTIDFKEKELKAFKDKVDQDIDSTNKQRKELNELLNENKLLQQSLIERERAINSKDSLLNKKIETIKRQLHDKEMRVLRLVDRMKLAEQKYQTEINRLRTQTFDSEKQDIKNFFPPLFKINGNDMAFPYLYP", "aa_hexdigest": "b6ff1330878229225a030002b5dc376f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37801.716900000036, "isoelectric_point": 9.508780479431152 }, "id": "KKNAAMEMLJ_491", "locus": "GCA000027325_00464" }, { "type": "cds", "sequence": "contig_1", "start": 264118, "stop": 264594, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAACGCTATGAAAATGAGTTAAATGAATTGCGTAGACAACGTAATTTACTTGAGAAAAAACTTGATCAAATCCAACTAGAATCCCAACTTAATAATAAGCAAAGTGAGTTTAGTAAGGTGGAATCAATGATGGAAAAACTACTTGAAAAAACTGAGAGTAGGTTAAATGATTTTGATCAGAAAATTAACTATCTCACCAAGAAAGTTAACCAACACAACACCTATCAACCAAGTTCCTATCAACCAACTCCTTCTTATCAAGACAGTGATAAACAACAGTTGTTATTTAGAATCCAAGAACTGGAAAAACAAAACTTATTCCAACAACAATTTCAACCTGCACCAGCTGTTGTCCAACAACCTACTAGTTTTGCAGCCCCTAACATCACCAAACAACAGCAAATTGCCCAACTTAATGCTGAAATTAACAACATTAAAAGGTTGATTGCCCAAAAAGCAGCAAGTAAATAA", "aa": "MQQRYENELNELRRQRNLLEKKLDQIQLESQLNNKQSEFSKVESMMEKLLEKTESRLNDFDQKINYLTKKVNQHNTYQPSSYQPTPSYQDSDKQQLLFRIQELEKQNLFQQQFQPAPAVVQQPTSFAAPNITKQQQIAQLNAEINNIKRLIAQKAASK", "aa_hexdigest": "c866201a75b0dae332d05662976d194c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18683.82479999999, "isoelectric_point": 9.245685005187987 }, "id": "KKNAAMEMLJ_492", "locus": "GCA000027325_00465" }, { "type": "cds", "sequence": "contig_1", "start": 264597, "stop": 265292, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG218-1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB78", "UniRef:UniRef90_Q9ZB78" ], "nt": "ATGGTTAATAATGAATATCAACAACTAAACACTTTAGTTGAGAGTGATGATGAAGCGGATCTTGTGATTGCTAACCTAGTTAAACAACTCAATGAACTAAAGCAAATCCTTGTTTCACTAGATAATCAAGAAGCAAGTGCCACTGCAGTTACTGATAAAAAGGAAGAGGAATACAACCAAAACCAATCCAGTTTCCATAACTTCAGCAAAGAAACACTGCAAAAGCAAGCAAAACGTGGTTTTCTTTTACTGGAACGCTGTTCGTTGGTTGGGTTACAACAACTAGAGTTGGAGTATGTTAATTTGTTGGGCAGAAGTTTTGATTCTTATCAACAAAAAACAGAGCTTTTAAACAACTTAAAGGAGCTTGTTGATGAACATTTCAGTGATACTGAAAAAATTATCAATACCCTTGAAAAGATCTTTGATGTTATTGGCGGTAGTGAATATACCCCTGTCTTAAACTCGTTTTTTAACAAGCTTTTAAGTGATCCTGATCCAATCCAACGGGAAATTGGCTTAAGACAATTTATCATCACTCTCCGTCAGCGCTTTAAAAAGTTATCACAAAAGATTGACAGTTCTCTCAAACAGATAGAAACAGAGGCTAAAATAGCCACTGAACAGGTTCAAAATAGTGAAGTGATGTTCGGTCCCCCTGATATTGCTAATGATCATGAGTTAAACCTGAACTGA", "aa": "MVNNEYQQLNTLVESDDEADLVIANLVKQLNELKQILVSLDNQEASATAVTDKKEEEYNQNQSSFHNFSKETLQKQAKRGFLLLERCSLVGLQQLELEYVNLLGRSFDSYQQKTELLNNLKELVDEHFSDTEKIINTLEKIFDVIGGSEYTPVLNSFFNKLLSDPDPIQREIGLRQFIITLRQRFKKLSQKIDSSLKQIETEAKIATEQVQNSEVMFGPPDIANDHELNLN", "aa_hexdigest": "13caf5b023013f741b427089ab755c8f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 264597, "stop": 265595, "observations": { "mutation": [ 265290 ], "stop": [ 265290 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZB78", "query_cov": 1.0, "subject_cov": 0.6676300578034682, "identity": 1.0, "score": 431.0, "evalue": 2.07e-150, "gene_start": 1, "gene_end": 231, "reference_start": 1, "reference_end": 231, "reference_sequence": "MVNNEYQQLNTLVESDDEADLVIANLVKQLNELKQILVSLDNQEASATAVTDKKEEEYNQNQSSFHNFSKETLQKQAKRGFLLLERCSLVGLQQLELEYVNLLGRSFDSYQQKTELLNNLKELVDEHFSDTEKIINTLEKIFDVIGGSEYTPVLNSFFNKLLSDPDPIQREIGLRQFIITLRQRFKKLSQKIDSSLKQIETEAKIATEQVQNSEVMFGPPDIANDHELNLNWPDSETDAILSSMENELEAALLAKHQEEPPLIVTPPSLIKPTVSQPEVEVVTPTNNTNFQPQVDLKPTDLKKQQKKKPLNFITRPVFKSNLPPKLSKDDIVHYAHQLLEKNTHNE" }, "paralog": false, "identity": 0.9969879518072289, "score": 644.0, "evalue": 4.51e-237, "description": "Internal stop codon at 265290. Nonsense mutation around 265290.", "psc": { "uniref90_id": "UniRef90_Q9ZB78", "product": "Uncharacterized protein MG218.1", "uniref50_id": "UniRef50_Q9ZB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZB78", "UniRef:UniRef50_Q9ZB78" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB78" ], "product": "Uncharacterized protein MG218.1" } }, "id": "KKNAAMEMLJ_493", "locus": "GCA000027325_00466" }, { "type": "cds", "sequence": "contig_1", "start": 265326, "stop": 265637, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D5328C", "UniRef:UniRef90_UPI0002D5328C" ], "nt": "ATGGAAAATGAATTGGAAGCTGCTTTATTAGCAAAACACCAAGAAGAACCACCGTTAATTGTTACCCCACCCAGCTTAATAAAACCAACTGTTAGTCAACCTGAAGTTGAAGTTGTTACACCTACTAACAACACTAATTTCCAACCCCAAGTTGATCTCAAACCTACTGATTTGAAAAAACAACAGAAGAAAAAACCACTTAACTTTATTACCCGTCCTGTTTTCAAAAGTAATTTGCCACCGAAACTAAGTAAGGATGACATAGTTCATTATGCGCACCAGTTACTTGAAAAAAATACCCATAATGAATAG", "aa": "MENELEAALLAKHQEEPPLIVTPPSLIKPTVSQPEVEVVTPTNNTNFQPQVDLKPTDLKKQQKKKPLNFITRPVFKSNLPPKLSKDDIVHYAHQLLEKNTHNE", "aa_hexdigest": "4163eae9060dcff9184c604758f2730c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 265242, "stop": 265637, "observations": { "mutation": [ 265290 ], "stop": [ 265290 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002D5328C", "query_cov": 1.0, "subject_cov": 0.7923076923076923, "identity": 0.971, "score": 189.0, "evalue": 2.16e-60, "gene_start": 1, "gene_end": 103, "reference_start": 29, "reference_end": 130, "reference_sequence": "MFGPPDIANDHELNLNWPDSETDAILSSMENELEAALLAKHQEPPLIVTLPSLIKPTVSQPEVEVVTPTNNTNFQPQVDLKPTDLKKQHKKKPLNFITRPVFKSNLPPKLSKDDIVHYAHQLLEKNTHNE" }, "paralog": false, "identity": 0.9694656488549618, "score": 253.0, "evalue": 5.65e-88, "description": "Internal stop codon at 265290. Nonsense mutation around 265290.", "psc": { "uniref90_id": "UniRef90_UPI0002D5328C", "uniref50_id": "UniRef50_UPI0002D5328C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002D5328C", "UniRef:UniRef50_UPI0002D5328C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002D5328C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D5328C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11736.33639999999, "isoelectric_point": 6.913292503356933 }, "id": "KKNAAMEMLJ_494", "locus": "GCA000027325_00467" }, { "type": "cds", "sequence": "contig_1", "start": 265597, "stop": 266043, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG219", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139494", "UniRef:UniRef100_P47461", "UniRef:UniRef50_P47461", "UniRef:UniRef90_P47461" ], "nt": "ATGCGCACCAGTTACTTGAAAAAAATACCCATAATGAATAGTGATAGTGATCTAAAACTCCAAAAGGTGTGGATCGAGCGGCATGTTGATCAAGATGAACTTAGTTTAACAACTACTGCAGTTGAACTTAAAAAGAGTGATGAACAAAAACCTGTTGCCATTAAAAGTAGTGACTTTATTGGTCATGAAGAGTTAATCTCTGTTCCAGTTTTACTAATCCCAACCCCTGTTGTTAAAGAGATTGATCAACCAGCAGTTATTCCTCCAGTTAAAGCAAAACCAAAAGCAACTAAAAAGAAAACTCCTGTTAAATCAAAACCAACTAGTAAATCAACTAAACAAACAAAACCTAAACAATCCAAGCCCAAATCAAAACAAGTTCAACAAACCAAAGCTAAACCAACCCAAATTCAAACAAAAAAAAGCAATAAAAAAACCAGATCTTAA", "aa": "MRTSYLKKIPIMNSDSDLKLQKVWIERHVDQDELSLTTTAVELKKSDEQKPVAIKSSDFIGHEELISVPVLLIPTPVVKEIDQPAVIPPVKAKPKATKKKTPVKSKPTSKSTKQTKPKQSKPKSKQVQQTKAKPTQIQTKKSNKKTRS", "aa_hexdigest": "e1e36d938f30c2936c53becc82207197", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139494", "uniref100_id": "UniRef100_P47461", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139494", "UniRef:UniRef100_P47461" ] }, "ips": { "uniref100_id": "UniRef100_P47461", "uniref90_id": "UniRef90_P47461" }, "psc": { "uniref90_id": "UniRef90_P47461", "product": "Uncharacterized protein MG219", "uniref50_id": "UniRef50_P47461" }, "pscc": { "uniref50_id": "UniRef50_P47461", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47461" ], "product": "Uncharacterized protein MG219" }, "genes": [], "id": "KKNAAMEMLJ_495", "locus": "GCA000027325_00468" }, { "type": "cds", "sequence": "contig_1", "start": 266075, "stop": 266278, "strand": "-", "frame": 2, "gene": null, "product": "MPN313 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q49403", "UniRef:UniRef90_Q49403" ], "nt": "ATGATAGTTAATGCACTTACTGGGGTTGCACATGCAGTAAATGACATTTTTCAAAGTACCACAGCTAATGCCAATGGTTCTGATGATGATAATGAGAATAAAAATAATAGTTATCGCTCTAAAAGCAATTACTTTAACACCGCACGGTTTAAACTAGGATTAACCCCAGGTAGTTCTTCCTATTCCTTTCCAGTTTTTAGTTAA", "aa": "MIVNALTGVAHAVNDIFQSTTANANGSDDDNENKNNSYRSKSNYFNTARFKLGLTPGSSSYSFPVFS", "aa_hexdigest": "ef1995f1d2b9f9a0ae7f978eeed0a5e7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 266075, "stop": 266356, "observations": { "mutation": [ 266302 ], "stop": [ 266302 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49403", "query_cov": 1.0, "subject_cov": 0.7204301075268817, "identity": 1.0, "score": 107.0, "evalue": 6.54e-29, "gene_start": 1, "gene_end": 67, "reference_start": 27, "reference_end": 93, "reference_sequence": "MYKLEKAQAKQVVGGLSFWTFSAGLIMIVNALTGVAHAVNDIFQSTTANANGSDDDNENKNNSYRSKSNYFNTARFKLGLTPGSSSYSFPVFS" }, "paralog": false, "identity": 0.989247311827957, "score": 154.0, "evalue": 2.1e-50, "description": "Internal stop codon at 266302. Nonsense mutation around 266302.", "psc": { "uniref90_id": "UniRef90_Q49403", "product": "MPN313 family protein", "uniref50_id": "UniRef50_Q49403", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49403", "UniRef:UniRef50_Q49403", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q49403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49403" ], "product": "Uncharacterized protein MG220" } }, "id": "KKNAAMEMLJ_496", "locus": "GCA000027325_00469" }, { "type": "tRNA", "sequence": "contig_1", "start": 266423, "stop": 266499, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(gcg)", "amino_acid": "Arg", "anti_codon": "gcg", "score": 65.2, "nt": "GTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 266457, 266459 ], "id": "KKNAAMEMLJ_9", "locus": "GCA000027325_00470" }, { "type": "cds", "sequence": "contig_1", "start": 266627, "stop": 267091, "strand": "+", "frame": 2, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143", "SO:0001217", "UniRef:UniRef50_P47463", "UniRef:UniRef90_P47463" ], "nt": "GTGTTTAAAAGTGTCGCAAAGTGTGACAAAGTGGAAAAAATGCTGCTAGGTACCTTTAATCTTACCCTTGATAACAAGAACAGAATTAGCTTGCCAGCTAAGCTCCGTAGTTTCTTTGATAGCAGCATAGTTATTAACCGCGGCTTTGAAAACTGTTTGGAAATTAGAAAACCTGCAGACTTTGAGAGTTATTTTCAAACCTTTAATAACTTCCCTAACACCCAAAAAGACACAAGAACATTAAAACGCTTAATCTTTGCTAATGCTAATCTAGTTGAACTTGATAGTGCAAACAGAATCCTAATCCCTAATAACCTAATTAGTGATGCTAAGTTAGATAAAGAGATCGTGTTAATTGGTCAATTTGACCATCTTGAAGTTTGGGATAAAGTGCAATATGAACAATATCTAGCTAGTTCAGAATCACTAGAGACAGTAGCTGAAAGGATGAAAGATGCTAAATAA", "aa": "MFKSVAKCDKVEKMLLGTFNLTLDNKNRISLPAKLRSFFDSSIVINRGFENCLEIRKPADFESYFQTFNNFPNTQKDTRTLKRLIFANANLVELDSANRILIPNNLISDAKLDKEIVLIGQFDHLEVWDKVQYEQYLASSESLETVAERMKDAK", "aa_hexdigest": "e5c898d6258936bf40e8c63458aea833", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47463", "query_cov": 0.9155844155844156, "subject_cov": 1.0, "identity": 1.0, "score": 276.0, "evalue": 8.68e-94, "valid": true, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "uniref50_id": "UniRef50_P47463", "cog_id": "COG2001", "cog_category": "J", "go_ids": [ "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143" ] }, "pscc": { "uniref50_id": "UniRef50_P47463", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47463" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "KKNAAMEMLJ_497", "locus": "GCA000027325_00471" }, { "type": "cds", "sequence": "contig_1", "start": 267081, "stop": 268010, "strand": "+", "frame": 3, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424", "RefSeq:WP_009885760.1", "SO:0001217", "UniParc:UPI000012F4EC", "UniRef:UniRef100_P47464", "UniRef:UniRef50_P47464", "UniRef:UniRef90_P47464" ], "nt": "ATGCTAAATAACCAACAGATCCACCAGAGTGTACTGATCAATGAAGTGATCCATAACCTCAATATTAACCCTTGTGGTAACTATTTAGATCTAACTGCAGGGTTTGCAGGACACAGTCAAAAGATCTTAGAAAAACTAACAACAGGAACTTTAACAATTAATGATGTTGATAAAGAAAGTATTAATTTTTGCCAAAAGCTTTTTTTTAAAAACAACAACGTTGTTATTATTCACGATAACTTTGCTAACTTCCCAGTTCATCTTAAACAACTATCAATAACCAAGTTTGATGGGATCTTAATGGACCTTGGTGTATCAAGCCATCAACTCAACCAACCTAATCGCGGTTTTAGTTTTAAGAATGATGGACCGATTGACATGCGTATGGACCAATCCAATCAGAAAAATACCGCACTAACAGTTTTAAAAAACTTAACTGAACAAAAGTTAAGTCTAATCCTTAAAAAGTATGGTGATATTAAACACCCTAAACCAATTGCTATTGGATTGAAAAAAGCAGTTCAAACTGAAAAAAATCTTACCACAACTCAACTAGCAAAAGTGGTAAAAGAATGTGCTACTGGATTTGAAAAATACCAATCAAGAAACTATCTTGCCAAAGTTTTTCAAGCAATTAGGATCTATCTTAATGATGAGATTACTAATCTGAAAACTGCGTTAACTTTTATCCCTAATCTTTTAAAAAACAACAGCAGGTTTCTTGTGATTGTTTTTCACTCCATTGAAGAAAAAATTGTAAGGAATTTCATTGCAAAACTAACCAGCTTTATCCAACCTGAAGCTCTACCCATTAAACTCACTCCTGCTTACCAGTTAATTACAAAAAAACCAATCCTACCTTCCCAAAAAGAACTTGAATTAAACCCGCGTTCGCGTAGTGCCAAACTCTTTGTTATCCAAAAAAACTAG", "aa": "MLNNQQIHQSVLINEVIHNLNINPCGNYLDLTAGFAGHSQKILEKLTTGTLTINDVDKESINFCQKLFFKNNNVVIIHDNFANFPVHLKQLSITKFDGILMDLGVSSHQLNQPNRGFSFKNDGPIDMRMDQSNQKNTALTVLKNLTEQKLSLILKKYGDIKHPKPIAIGLKKAVQTEKNLTTTQLAKVVKECATGFEKYQSRNYLAKVFQAIRIYLNDEITNLKTALTFIPNLLKNNSRFLVIVFHSIEEKIVRNFIAKLTSFIQPEALPIKLTPAYQLITKKPILPSQKELELNPRSRSAKLFVIQKN", "aa_hexdigest": "79f1d9d9df40110e3ed1410304d3d671", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012F4EC", "ncbi_nrp_id": "WP_009885760.1", "uniref100_id": "UniRef100_P47464", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012F4EC", "RefSeq:WP_009885760.1", "UniRef:UniRef100_P47464" ] }, "ips": { "uniref100_id": "UniRef100_P47464", "uniref90_id": "UniRef90_P47464" }, "psc": { "uniref90_id": "UniRef90_P47464", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_P47464", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ] }, "pscc": { "uniref50_id": "UniRef50_P47464", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47464" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "KKNAAMEMLJ_498", "locus": "GCA000027325_00472" }, { "type": "cds", "sequence": "contig_1", "start": 268012, "stop": 269247, "strand": "+", "frame": 1, "gene": null, "product": "MPN316 family protein", "db_xrefs": [ "RefSeq:WP_010869381.1", "SO:0001217", "UniParc:UPI00001394A5", "UniRef:UniRef100_P47465", "UniRef:UniRef50_P47465", "UniRef:UniRef90_P47465" ], "nt": "ATGTACAAACCAAAAAATATTAACAGCGTATTAACCTTTTATAAGGATCAGATCCAACTGGTTGTTAGTGATGATCAAAACCAGTTCAACATCTTGTTTTACCAAACAATTGATAACGATGGCTTTTATTCAAAACAACAGTTGAAAAACAAACTAAGACTCAAGTTAGCATTAAACCAACTAGTTGATCAAGCTAACTATTTTCTTGGTTTTAAACTGGAAAAGGTAGTTGTTGTTCTCGCTGAACTGATTGATGATTTGAAGATCCATAATTTCAAGAGTGAGATCTTTTTTACTGGTTATGATTTTGATCATAAAGCGATGATTAAAAAAGAAAAACAACGCTTTTGTGAGCAAAATAACCAACTAACAGTTATGGATACAATGGTTTTAAACTACCATGATGTTATTAACAATAAGATCACCAAAAGCTTTGCATTTAACAAGAGCTATGTAGCTAATTTAGTGGCATATTCCTCTAAAAGTAACCTGATCGGGGAGTTGAAGTTCTTTTTAAAAAGAAACGTTAATCTTAAGGTTAAGAAAATTATTAGTCACCACTTAGCATTAGCCAACTCCTTAAGTAAGAAACAAAACAACATGTTTGTTTATTTAGGACAAAAAACTACTGAACTGATGCTATTTATGGACAATGCTTTAGTTGATGTTATTACCAACCAGTTTGGTAAAAACCACTTTATTGATATTCCAGCTAACCAGGAAAACAAACCACTGCTTGAGTTTTTAGTTGATAACACCACTAAGATTGGTGATTGTTATTCGCTTGGTATGACCTATACAGATGGTGATAGTTACAAAGAGATTAAGGCTTTGACTATTGGTGATTTAATGCAAACAGTTAGTGACAAGATCAAAACCTTAATTGATTTTATTAACAGTGGTTCTCTAACTTTTTTCAACAAGTTTAAAACCTTACCTAAGCTATTGTATTTTTATACAAGATCAAAACAAATTACCAACCTTTTTCAAGCTAATGTTGCACTTATCAATCCCCAGTTTAAAACTGTTGATATTTATAAGAACAAGATCCAGTTTATTAGTGAAAACTACCTGTTAAGCTGTGAAGCGATTAGCTTGCAGATTACCAATAGAATCAAAAACCAAATTAGTTTTGATTTCACAAATGCTGATAATATTCAAAAACCTAAACCAAAAAAACACTTCATGATCTTATCAAAACACCTAACAAAGTTTGTCCAACGCTTGGTTAAATAA", "aa": "MYKPKNINSVLTFYKDQIQLVVSDDQNQFNILFYQTIDNDGFYSKQQLKNKLRLKLALNQLVDQANYFLGFKLEKVVVVLAELIDDLKIHNFKSEIFFTGYDFDHKAMIKKEKQRFCEQNNQLTVMDTMVLNYHDVINNKITKSFAFNKSYVANLVAYSSKSNLIGELKFFLKRNVNLKVKKIISHHLALANSLSKKQNNMFVYLGQKTTELMLFMDNALVDVITNQFGKNHFIDIPANQENKPLLEFLVDNTTKIGDCYSLGMTYTDGDSYKEIKALTIGDLMQTVSDKIKTLIDFINSGSLTFFNKFKTLPKLLYFYTRSKQITNLFQANVALINPQFKTVDIYKNKIQFISENYLLSCEAISLQITNRIKNQISFDFTNADNIQKPKPKKHFMILSKHLTKFVQRLVK", "aa_hexdigest": "9cd5851d12c857a80d991e52316963bc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394A5", "ncbi_nrp_id": "WP_010869381.1", "uniref100_id": "UniRef100_P47465", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394A5", "RefSeq:WP_010869381.1", "UniRef:UniRef100_P47465" ] }, "ips": { "uniref100_id": "UniRef100_P47465", "uniref90_id": "UniRef90_P47465" }, "psc": { "uniref90_id": "UniRef90_P47465", "product": "MPN316 family protein", "uniref50_id": "UniRef50_P47465" }, "pscc": { "uniref50_id": "UniRef50_P47465", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465" ], "product": "Uncharacterized protein MG223" }, "genes": [], "id": "KKNAAMEMLJ_499", "locus": "GCA000027325_00473" }, { "type": "cds", "sequence": "contig_1", "start": 269250, "stop": 270140, "strand": "+", "frame": 3, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258", "GO:0051301", "KEGG:K03531", "SO:0001217", "UniRef:UniRef50_P47466", "UniRef:UniRef90_P47466" ], "nt": "ATGGATGAAAATGAAACTCAATTCAACAAGTTAAACCAAGTTAAAAACAAGCTGAAAATTGGTGTTTTTGGGATTGGAGGTGCTGGTAATAACATTGTTGATGCATCACTTTATCACTATCCTAATTTAGCAAGTGAAAACATCCACTTTTATGCTATAAATTCAGATTTACAACACCTTGCATTTAAAACGAATGTTAAAAATAAACTCTTAATTCAAGACCATACTAACAAGGGCTTTGGAGCGGGGGGTGATCCAGCTAAAGGAGCTAGTTTAGCAATAAGCTTTCAAGAACAGTTTAATACACTTACAGATGGGTATGATTTTTGTATCTTAGTTGCTGGATTTGGTAAGGGTACTGGTACAGGTGCTACCCCAGTTTTTAGCAAGATCTTAAAAACTAAGAAGATCTTAAATGTTGCTATTGTTACCTATCCATCTTTAAACGAGGGATTAACAGTGAGAAACAAAGCCACTAAGGGGCTTGAAATTCTCAACAAAGCAACTGATAGTTACATGCTATTTTGTAATGAAAAATGTACAAATGGTATCTACCAACTAGCAAACACAGAGATAGTCAGTGCCATTAAAAACCTAATAGAACTAATTACTATTCCTTTGCAGCAAAACATTGATTTTGAAGATGTACGTGCCTTTTTTCAAACCAAAAAAACTAACCAAGATCAACAGCTTTTTACTGTTACTCACCCCTTTAGTTTTAGCTTTGATAGTAAAGATAGTATAGAACAGTTTGCTAAACAGTTTAAGAACTTTGAAAAAGTTAGTTATTTTGACCACTCTATAGTAGGAGCTAAAAAAGTGTTATTGAAAGCTAACATTAACCAAAAGATAGTCAAGCTTAACTTCAAGCAGATCCAAGATATTATCTGA", "aa": "MDENETQFNKLNQVKNKLKIGVFGIGGAGNNIVDASLYHYPNLASENIHFYAINSDLQHLAFKTNVKNKLLIQDHTNKGFGAGGDPAKGASLAISFQEQFNTLTDGYDFCILVAGFGKGTGTGATPVFSKILKTKKILNVAIVTYPSLNEGLTVRNKATKGLEILNKATDSYMLFCNEKCTNGIYQLANTEIVSAIKNLIELITIPLQQNIDFEDVRAFFQTKKTNQDQQLFTVTHPFSFSFDSKDSIEQFAKQFKNFEKVSYFDHSIVGAKKVLLKANINQKIVKLNFKQIQDII", "aa_hexdigest": "af84eeb795d1c95ddba768062f8d4188", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47466", "query_cov": 1.0, "subject_cov": 0.8021680216802168, "identity": 1.0, "score": 566.0, "evalue": 1.65e-202, "valid": true, "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_P47466", "cog_id": "COG0206", "cog_category": "D", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_P47466", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47466" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "KKNAAMEMLJ_500", "locus": "GCA000027325_00474" }, { "type": "cds", "sequence": "contig_1", "start": 270189, "stop": 270359, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAACCATCCCTAATATCCAAATTTTTATCCTCAGTGAACACAAAAATCCAGTTTCGCTTCCCATTGATAATAAATCAACTGAAAACAACCAAAATAAGTTGAAACTTTTAGATGAGCTGAAAGAACTTGGCATGAAATATGTTAAGCACCAAAACCAAATCTACTAA", "aa": "MTTIPNIQIFILSEHKNPVSLPIDNKSTENNQNKLKLLDELKELGMKYVKHQNQIY", "aa_hexdigest": "597266b74abfa706df615d518737eda1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6565.529300000002, "isoelectric_point": 8.050183296203613 }, "id": "KKNAAMEMLJ_501", "locus": "GCA000027325_00475" }, { "type": "cds", "sequence": "contig_1", "start": 270405, "stop": 270692, "strand": "+", "frame": 3, "gene": null, "product": "amino acid permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8" ], "nt": "ATGGGTCAAATCAATCGGAAGTTTAGCGAAAAGCAGTTCTTACTTTTTGTTGTTAACTATATTGCTGGATTTGGCTTTATTGCTACTGCTATCTCACTGTTTCGCTTAGGACCTTTTTCTTGGTTAATCTTTCTGCTTGTTAGCTTAGTTAGTTTAATTGTTACCTTATCATTCGCACGGCTTTCATCAATAGATAGTCAAAACTATGGTGGGCCTTATCTTTGGGCTAAGAAAGCGGTTGATAAAGAGAAGATAGCAGGGAGAATGTTTAGCTTTTTTACGGGGTGA", "aa": "MGQINRKFSEKQFLLFVVNYIAGFGFIATAISLFRLGPFSWLIFLLVSLVSLIVTLSFARLSSIDSQNYGGPYLWAKKAVDKEKIAGRMFSFFTG", "aa_hexdigest": "9aa49a3a7eaf04b55bdcdf088691fde6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027DFE8A8", "query_cov": 0.9263157894736842, "subject_cov": 1.0, "identity": 0.67, "score": 122.0, "evalue": 1.42e-34, "valid": false, "product": "amino acid permease", "uniref50_id": "UniRef50_UPI0027DFE8A8" }, "pscc": { "uniref50_id": "UniRef50_UPI0027DFE8A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8" ], "product": "amino acid permease" }, "genes": [], "id": "KKNAAMEMLJ_502", "locus": "GCA000027325_00476" }, { "type": "cds", "sequence": "contig_1", "start": 270620, "stop": 270793, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACTAAGTTACCTCTAATCCCATCAATACCACTAAAGGAATTGAGGATAAAAAGTGGTGCAGTTGCTGCTGAAAGAGGACCAATGATAAAGTTATTTCACCCCGTAAAAAAGCTAAACATTCTCCCTGCTATCTTCTCTTTATCAACCGCTTTCTTAGCCCAAAGATAA", "aa": "MLTKLPLIPSIPLKELRIKSGAVAAERGPMIKLFHPVKKLNILPAIFSLSTAFLAQR", "aa_hexdigest": "dc4015c7b6b0a5f0d0550e2cd38aeccb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6279.680499999999, "isoelectric_point": 11.165940284729004 }, "id": "KKNAAMEMLJ_503", "locus": "GCA000027325_00477" }, { "type": "cds", "sequence": "contig_1", "start": 270909, "stop": 271874, "strand": "+", "frame": 3, "gene": null, "product": "APC family permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2" ], "nt": "GTGATCCTCTCAGCACTAATAGTAGCAATCTATGTTATTGCTAGAGATGGTAATGGTTATAGTCAAAACAATAACTTAGAAAGTGGTTTTTTTGGGAGAAGAGAGATTAGTTTTGCACAGATAGCAACGGTATTTATTACCTTCTTTTATTCTTATGCAGGGGTTGAAGATATCTCAGTGATGACTCCTGATGTTAAAACTAATAACTTTAGAAAGATATTAATTGTCTCTTTTATAGCAGTTTTCCTCTTTTATTTCATTGGGATTATTATTCTAAATGGTTTGCAAAACATTGCTCAAAGAGGTGGGGAAGCCAATTCAATTGGTAATGTAGCGGATATCTTTAAAAAAGCTGCTGGGCTTGGGACTTTAATCTTTTATGGAGTTGGAGCATTGTTTAACAATGTCTCAACCAGACTTTCAACTATTATTGCCAACTCCAGAAAGATTCTTCCGCTTGCTTATGATAACTATTTACCTAGTTTCTTTTACAAGCAAAACAAAAAAGGTGAGTTTCAGAATGCAATTTGGTTTACCTTTGGTACTACTTTAATTGCAATGACTTTGCTTGTCTTTATCCCTTTAGTTGCTTCTAACTTTGATTTTGATAATGCTACTGAGTATGCAGCATCTGTTGGCTCAGCTGCAACTTTGCTACAATATATTTTTGTTTTTTTTATAATCTTTAAGTTTATCTATAAAAAAGAACCACTCTACCAGAAAAAATGGGTTAAAACAACTGAAGAATTATTGTTTTGTTTAGGAACAATTGTCATTGTTTTAATGTTGTTGGTTTATCTGTTTCCTGTTATAGATGGATTTTCAAAATGGGAAACTAAACACACATTAACAATTGTGTTATATGGGGTTTTAAGCCTGATAGGATTGGTACTTTTTTTGCTCCAAGAATACAAACATAAAAACAAGCAAAATGCAAACAAACAAACAACCCAAACAACAGTTTAG", "aa": "MILSALIVAIYVIARDGNGYSQNNNLESGFFGRREISFAQIATVFITFFYSYAGVEDISVMTPDVKTNNFRKILIVSFIAVFLFYFIGIIILNGLQNIAQRGGEANSIGNVADIFKKAAGLGTLIFYGVGALFNNVSTRLSTIIANSRKILPLAYDNYLPSFFYKQNKKGEFQNAIWFTFGTTLIAMTLLVFIPLVASNFDFDNATEYAASVGSAATLLQYIFVFFIIFKFIYKKEPLYQKKWVKTTEELLFCLGTIVIVLMLLVYLFPVIDGFSKWETKHTLTIVLYGVLSLIGLVLFLLQEYKHKNKQNANKQTTQTTV", "aa_hexdigest": "9f336aecad6c91a17b0919ba8400a788", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FB1D5B2", "query_cov": 0.9750778816199377, "subject_cov": 0.8528610354223434, "identity": 0.687, "score": 422.0, "evalue": 3.31e-145, "valid": false, "product": "APC family permease", "uniref50_id": "UniRef50_UPI001FB1D5B2" }, "pscc": { "uniref50_id": "UniRef50_UPI001FB1D5B2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2" ], "product": "APC family permease" }, "genes": [], "id": "KKNAAMEMLJ_504", "locus": "GCA000027325_00478" }, { "type": "cds", "sequence": "contig_1", "start": 271840, "stop": 273117, "strand": "+", "frame": 1, "gene": "potE", "product": "putative protein MG226", "db_xrefs": [ "COG:COG0531", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47468", "UniRef:UniRef90_P47468" ], "nt": "ATGCAAACAAACAAACAACCCAAACAACAGTTTAGTGAAAAGCAATTCATTGCTTTTGTCTTTAACTATATTGCTGGATTTGGCTTTATATCAGTGGTGATGACCATGTTTGATGTTGGGCCATTTTCCTATCTTGTTTTAGGGTTAACTTCGTTTGCTATTTTAGGAGTTGTGCTTTCTTTTTCTCGCCTTTCAGTTCTCTGTGGTAATAGTGCTTATGGAGGGAGTTATTTAATTGCTAAAAAAGCAGTTGGTACTAACAGTAAAACAAAAAGGTTTTTTGTTTTTTTAAGTGGGTGGAATGTATCGTTAACAGGATCTTTTAACGGTGTTGTTATTCCAGCAGTATTAATCTTTTCCTTTGCAGATATTCCAGTAGTTAAAGCGAATAATAACATCATTATTGGCCTTTTAGTAGGTGGGTTTTTGTTGTTTGGCTTACTTACTTTTATCTCGTTATTTGGTTTAAAAATTAACAAGAAAGCAATCTTTTATTTTGCTGTTATTAAGTGGATAGTAGTAATAGGTGGGTTTATCTTAGGGATCTATTTAATTGGTACTACCAATGGTAAAGGTTTTGTTGAAAACAATTTAATTGGGACTAGGGAAAACATTGATTTTTTCAAGATTATCTTTATTAGTCTGGCTTTAACCATTGCTTTTGCAGGGACAGAGGATTTAGCTTCGATTACTCCTGATGTCAAGTCAAATAACTTAAGAAAGTGTTTTTTAATTGCCTTTGGGTGTGTTGTGTTACTTTACCTAGTTGGGTTTGTTATTATCAGTGGACTTGATGGGATTAGAGGTTATGGATTAGCATTAGGTAATAAAGATCCCAAGGCAATTAATAACTATGGATCTATCTACCGTTTGGTAGGAGGAGTTCCTTTACTTGTTATCTATGGACTTGGGTTACTTGTCAATTCCTTAGCATCACGCCTATCAATGACAATTACAACAGCTAGAAAATATGTAGCTTTAGCTCAAGATGGGTTTTTACCCTCTTTTTTAGCAAAAACTAATAAACATAATGAGTATCATCATGCAGTTTTAATTAGTAATCTAATGACTTTATTAGTGATGCTAATTATGGTAATAATCCCCTTTTTACCAGACCATAACAACAATAACAATAGTTTGTTTAATGCTATTGAACAGTTGGTTACAGTTACCATTGAAATGGCTGCAGCCATTTCTTTGATCCAATACTTTATTACCTTTATCTTCTTTTTTATGATCTTTGCTAAAAAGGAAAACCAGAAGTTAATTCCCTTGTGA", "aa": "MQTNKQPKQQFSEKQFIAFVFNYIAGFGFISVVMTMFDVGPFSYLVLGLTSFAILGVVLSFSRLSVLCGNSAYGGSYLIAKKAVGTNSKTKRFFVFLSGWNVSLTGSFNGVVIPAVLIFSFADIPVVKANNNIIIGLLVGGFLLFGLLTFISLFGLKINKKAIFYFAVIKWIVVIGGFILGIYLIGTTNGKGFVENNLIGTRENIDFFKIIFISLALTIAFAGTEDLASITPDVKSNNLRKCFLIAFGCVVLLYLVGFVIISGLDGIRGYGLALGNKDPKAINNYGSIYRLVGGVPLLVIYGLGLLVNSLASRLSMTITTARKYVALAQDGFLPSFLAKTNKHNEYHHAVLISNLMTLLVMLIMVIIPFLPDHNNNNNSLFNAIEQLVTVTIEMAAAISLIQYFITFIFFFMIFAKKENQKLIPL", "aa_hexdigest": "19bbd7d3277a58c4969b42ceb418756b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47468", "query_cov": 1.0, "subject_cov": 0.8638211382113821, "identity": 1.0, "score": 756.0, "evalue": 3.17e-273, "valid": true, "gene": "PotE", "product": "Uncharacterized protein MG226", "uniref50_id": "UniRef50_P47468", "cog_id": "COG0531", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47468", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47468" ], "product": "Uncharacterized protein MG226" }, "genes": [ "potE" ], "id": "KKNAAMEMLJ_505", "locus": "GCA000027325_00479" }, { "type": "cds", "sequence": "contig_1", "start": 273298, "stop": 273462, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_158302286.1", "SO:0001217", "UniParc:UPI0013052C28", "UniRef:UniRef100_UPI0013052C28", "UniRef:UniRef50_UPI0013052C28", "UniRef:UniRef90_UPI0013052C28" ], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGGGGGGTAGAAGAGGAAGCGGAAGTTGAAGGGGAGGGGGAGTTGGAGAAGGGTTTGTTGTTGCCGATTCAAAAGCAATTTCGCTTAATGAACTGTAGATTAGCTATTGTTATTCATTAA", "aa": "MIIDLTPTLMLEKVGRGVEEEAEVEGEGELEKGLLLPIQKQFRLMNCRLAIVIH", "aa_hexdigest": "ef7c4ec712353f4759b7a087d6a71f71", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0013052C28", "ncbi_nrp_id": "WP_158302286.1", "uniref100_id": "UniRef100_UPI0013052C28", "db_xrefs": [ "SO:0001217", "UniParc:UPI0013052C28", "RefSeq:WP_158302286.1", "UniRef:UniRef100_UPI0013052C28" ] }, "ips": { "uniref100_id": "UniRef100_UPI0013052C28", "uniref90_id": "UniRef90_UPI0013052C28" }, "psc": { "uniref90_id": "UniRef90_UPI0013052C28", "uniref50_id": "UniRef50_UPI0013052C28" }, "pscc": { "uniref50_id": "UniRef50_UPI0013052C28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013052C28" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6104.188599999998, "isoelectric_point": 4.783024024963378 }, "id": "KKNAAMEMLJ_506", "locus": "GCA000027325_00480" }, { "type": "cds", "sequence": "contig_1", "start": 273463, "stop": 273642, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTGACTTTATTATCAGAAGAATTGGGATTAAACGTGTTGCTACTACTACTAGGTTTCACCTGGTCAAGGAGATGCCATTATTGTTACCTTGTTTGCGATACCCTGCTCCTGTTCAGATGTCAGGTGAAAAGTTAGGTATAAACACCCACCGGGTGTTTTCTTTATTTAAATGTTGA", "aa": "MSDFIIRRIGIKRVATTTRFHLVKEMPLLLPCLRYPAPVQMSGEKLGINTHRVFSLFKC", "aa_hexdigest": "8df8558b28e62969502c07bf16f53412", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6831.2199, "isoelectric_point": 10.53015308380127 }, "id": "KKNAAMEMLJ_507", "locus": "GCA000027325_00481" }, { "type": "cds", "sequence": "contig_1", "start": 273790, "stop": 273972, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00303.24" ], "nt": "ATGAAACAGTATTTAGATTTAGCTAGTTATGTTTTAGCAAATGGTAAAAAAAGAAAAAACCGTACAGATACAGATACTTTAAGTGTCTTTGGTTACCAGATGAAATTTGACCTTACTAATAGTTTTCCTTTATTGACAACTAAAAAGGTTAATTGGAAGGCAATTGTCCATGAATTGTTGTGA", "aa": "MKQYLDLASYVLANGKKRKNRTDTDTLSVFGYQMKFDLTNSFPLLTTKKVNWKAIVHELL", "aa_hexdigest": "bb6b9ca0c6ac04d4b0d6b8e779825ef8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0037C09292", "query_cov": 1.0, "subject_cov": 0.9375, "identity": 0.6, "score": 75.1, "evalue": 1.01e-16, "valid": false, "product": "thymidylate synthase", "uniref50_id": "UniRef50_A0A967H814" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00303.24", "name": "Thymidylat_synt", "length": 60, "aa_cov": 0.9833333333333333, "hmm_cov": 1.0, "evalue": 7.207984302036317e-23, "score": 76.10872650146484, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 6995.086499999999, "isoelectric_point": 9.755372047424316 }, "id": "KKNAAMEMLJ_508", "locus": "GCA000027325_00482" }, { "type": "cds", "sequence": "contig_1", "start": 274282, "stop": 274653, "strand": "+", "frame": 1, "gene": "thyA", "product": "thymidylate synthase", "db_xrefs": [ "COG:COG0207", "COG:F", "EC:2.1.1.45", "GO:0004799", "GO:0005829", "GO:0006231", "GO:0006235", "GO:0032259", "KEGG:K00560", "SO:0001217", "UniRef:UniRef50_P47469", "UniRef:UniRef90_P47469" ], "nt": "ATGGCATTGGCTCCTTGTCATTCACTCTTTCAGTTCTATGTTGAAGAAGATAAACTAAGCTTACAGCTTTACCAGCGCAGCGGTGATATCTTTCTTGGTGTCCCATTTAACATTGCATCTTACGCCTTACTTGTGTATTTAGTTGCTCATGAAACTAAGTTAAAACCTGGTTATTTTATCCATACACTAGGAGATGCACATATCTATGAAAACCACATTGAACAAATTAAATTACAACTAACAAGAACAACCCTAGACCCCCCTCAAGTGGTTTTGAAAAGTGATAAATCAATCTTTGCTTATAGTTTTGATGATATTGAGTTAGTTGGTTATAATTACCATCCATTTATCTATGGGAGGGTTGCAGTTTAA", "aa": "MALAPCHSLFQFYVEEDKLSLQLYQRSGDIFLGVPFNIASYALLVYLVAHETKLKPGYFIHTLGDAHIYENHIEQIKLQLTRTTLDPPQVVLKSDKSIFAYSFDDIELVGYNYHPFIYGRVAV", "aa_hexdigest": "45fc626ecca00e240ef3748f49293db2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00260828A0", "query_cov": 1.008130081300813, "subject_cov": 0.8104575163398693, "identity": 0.629, "score": 156.0, "evalue": 1.25e-46, "valid": false, "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_A0A059X7H5", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0006231" ] }, "genes": [ "thyA" ], "truncated": "5-prime", "pseudogene": { "start": 273982, "stop": 274653, "observations": { "mutation": [ 274027, 274036, 274168, 274258 ], "stop": [ 274027, 274036, 274168, 274258 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47469", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 1.0, "score": 254.0, "evalue": 2.18e-83, "gene_start": 1, "gene_end": 123, "reference_start": 165, "reference_end": 287, "reference_sequence": "MKQYLDLASYVLANGKKRKNRTDTDTLSVFGYQMKFDLTNSFPLLTTKKVNWKAIVHELLWFIKGDTNIKYLVDNGVNIWNEWPYENFKKSPSFQNETLQEFILKVKTDNEFAKQFADLGPVYGKQWRNFNGVDQLKKVIQEIKENPNSRRLIVSSWNPSELEKMALAPCHSLFQFYVEEDKLSLQLYQRSGDIFLGVPFNIASYALLVYLVAHETKLKPGYFIHTLGDAHIYENHIEQIKLQLTRTTLDPPQVVLKSDKSIFAYSFDDIELVGYNYHPFIYGRVAV" }, "paralog": false, "identity": 0.9820627802690582, "score": 437.0, "evalue": 4.41e-158, "description": "Internal stop codon at 274027, 274036, 274168, 274258. Nonsense mutation around 274027, 274036, 274168, 274258.", "psc": { "uniref90_id": "UniRef90_P47469", "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_P47469", "cog_id": "COG0207", "cog_category": "F", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0005829", "GO:0006231", "GO:0006235", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47469", "EC:2.1.1.45", "UniRef:UniRef50_P47469", "COG:COG0207", "COG:F", "KEGG:K00560", "GO:0004799", "GO:0005829", "GO:0006231", "GO:0006235", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_P47469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47469" ], "product": "Thymidylate synthase" } }, "id": "KKNAAMEMLJ_509", "locus": "GCA000027325_00483" }, { "type": "cds", "sequence": "contig_1", "start": 274674, "stop": 274904, "strand": "+", "frame": 3, "gene": "folA", "product": "Dihydrofolate reductase", "db_xrefs": [ "COG:COG0262", "COG:H", "EC:1.5.1.3", "GO:0004146", "GO:0005829", "GO:0006730", "GO:0046452", "GO:0046654", "GO:0046655", "GO:0050661", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_P78028", "UniRef:UniRef90_P47470" ], "nt": "ATGACACAAGAAGGACTAATAGGTAATAACAACACTTTACCTTGGATGATTAAACAAGAGCTAGCTCACTTTAAAAAAACTACGTTATTTCAAGCTTTGTTAATGGGGAGAAAAACTTACGAATCACTCCCCAAGGTATTTGAAAAAAGAACAATATTTCTCCTTTCAAAAGATCAAAACTACCGTTTTGAAGAAAAGGGAAGTGAAGTGAAAGTTATTAATGATTTTTGA", "aa": "MTQEGLIGNNNTLPWMIKQELAHFKKTTLFQALLMGRKTYESLPKVFEKRTIFLLSKDQNYRFEEKGSEVKVINDF", "aa_hexdigest": "85ac8e532b79faa315a436b16ff85a8f", "start_type": "ATG", "rbs_motif": null, "genes": [ "folA" ], "truncated": "both", "pseudogene": { "start": 274653, "stop": 275135, "observations": { "mutation": [ 274668, 274902 ], "stop": [ 274668, 274902 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47470", "query_cov": 1.0, "subject_cov": 0.475, "identity": 1.0, "score": 154.0, "evalue": 1.32e-46, "gene_start": 1, "gene_end": 76, "reference_start": 8, "reference_end": 83, "reference_sequence": "MLIAIWAMTQEGLIGNNNTLPWMIKQELAHFKKTTLFQALLMGRKTYESLPKVFEKRTIFLLSKDQNYRFEEKGSEVKVINDFWPLIKSYQANKEKDLFICGGKSVYEQTINECDQLIVSIIKKKYKGDQFLKVDLSKFVLNEVVEFEEFNVNYYRKKQQ" }, "paralog": false, "identity": 0.9875, "score": 313.0, "evalue": 5.73e-112, "description": "Internal stop codon at 274668, 274902. Nonsense mutation around 274668, 274902.", "psc": { "uniref90_id": "UniRef90_P47470", "gene": "folA", "product": "Dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_P78028", "cog_id": "COG0262", "cog_category": "H", "kegg_orthology_id": "K00287", "go_ids": [ "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47470", "EC:1.5.1.3", "UniRef:UniRef50_P78028", "COG:COG0262", "COG:H", "KEGG:K00287", "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ] }, "pscc": { "uniref50_id": "UniRef50_P78028", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78028" ], "product": "Dihydrofolate reductase" } }, "id": "KKNAAMEMLJ_510", "locus": "GCA000027325_00484" }, { "type": "cds", "sequence": "contig_1", "start": 274944, "stop": 275135, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00186.24" ], "nt": "TTGTTTATTTGTGGTGGAAAAAGTGTGTATGAACAGACCATTAATGAATGTGATCAGTTAATTGTTTCAATCATTAAAAAGAAGTATAAGGGTGATCAGTTTTTGAAGGTTGATCTCAGTAAATTTGTACTTAATGAAGTTGTAGAGTTTGAGGAATTTAATGTTAATTATTATAGAAAGAAACAACAATAA", "aa": "MFICGGKSVYEQTINECDQLIVSIIKKKYKGDQFLKVDLSKFVLNEVVEFEEFNVNYYRKKQQ", "aa_hexdigest": "158254ec2552fe2e19ebf56e2f50e11b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00186.24", "name": "DHFR_1", "length": 53, "aa_cov": 0.8253968253968254, "hmm_cov": 1.0, "evalue": 7.731575138660066e-10, "score": 33.77817916870117, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 7516.623, "isoelectric_point": 7.714665412902832 }, "id": "KKNAAMEMLJ_511", "locus": "GCA000027325_00485" }, { "type": "cds", "sequence": "contig_1", "start": 275141, "stop": 275272, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCTAACAATAAAAAGTACTTTTTAGAATCATTTTCCCCACTTGGGTATGTAAAGAATAATTTTCAGGGCAACTTACGTTCTGTAAACTGGAATTTGGTTGATGATGAGAAGGATTTGGAAGTGTGA", "aa": "MAANNKKYFLESFSPLGYVKNNFQGNLRSVNWNLVDDEKDLEV", "aa_hexdigest": "a7567c754e2a481a928e9a97b07baeff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5008.533000000001, "isoelectric_point": 4.992987251281736 }, "id": "KKNAAMEMLJ_512", "locus": "GCA000027325_00486" }, { "type": "cds", "sequence": "contig_1", "start": 275492, "stop": 276163, "strand": "+", "frame": 2, "gene": null, "product": "Ribonucleoside-diphosphate reductase subunit beta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2I1IMZ3" ], "nt": "ATGGTAGGGGTACATGCCCGTTCCTATGGAACGATCTTCTCAACTTTATGTACATCAGAACAGATTAACGCTGCTCATGAGTGGGTTGTAAACACTGAAAGTCTCCAGAAAAGAGCAAAGGCATTAATCCCTTACTATACGGGCAATGACCCGTTAAAATCAAAGGTAGCAGCAGCTTTAATGCCTGGGTTTTTACTGTATGGTGGGTTTTATTTGCCTTTTTACTTGTCATCAAGAAAACAACTACCAAATACATCTGATATTATCCGCTTAATCCTTCGTGATAAAGTGATCCATAACTATTACAGTGGTTATAAATACCAACGTAAACTAGAAAAACTCCCTTTAGCAAAACAAAAGGAGATGAAAGCATTTGTTTTTGAACTAATGTATCGGTTAATTGAACTTGAAAAGGACTATTTAAAAGAGCTTTATGAAGGGTTTGGAATTGTTGATGATGCCATTAAGTTCAGTGTTTACAATGCTGGTAAGTTTTTACAGAACTTAGGTTATGACTCCCCGTTTACTGCAGCAGAAACCAGGATTAAACCAGAGATTTTTGCCCAACTATCAGCACGTGCTGATGAAAACCATGACTTTTTCTCAGGAAACGGTTCGTCGTATGTGATGGGAGTTAGTGAAGAGACAAATGATGATGATTGGAACTTTTAA", "aa": "MVGVHARSYGTIFSTLCTSEQINAAHEWVVNTESLQKRAKALIPYYTGNDPLKSKVAAALMPGFLLYGGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKYQRKLEKLPLAKQKEMKAFVFELMYRLIELEKDYLKELYEGFGIVDDAIKFSVYNAGKFLQNLGYDSPFTAAETRIKPEIFAQLSARADENHDFFSGNGSSYVMGVSEETNDDDWNF", "aa_hexdigest": "8cc6ab481636ff6e2182b5b833251dc4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X3CV73", "query_cov": 1.0, "subject_cov": 0.8383458646616542, "identity": 0.507, "score": 231.0, "evalue": 7.19e-73, "valid": false, "product": "ribonucleoside-diphosphate reductase", "uniref50_id": "UniRef50_A0A2I1IMZ3" }, "pscc": { "uniref50_id": "UniRef50_A0A2I1IMZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2I1IMZ3" ], "product": "Ribonucleoside-diphosphate reductase subunit beta" }, "genes": [], "id": "KKNAAMEMLJ_513", "locus": "GCA000027325_00487" }, { "type": "cds", "sequence": "contig_1", "start": 276167, "stop": 276628, "strand": "+", "frame": 2, "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "db_xrefs": [ "COG:COG1780", "COG:F", "GO:0009263", "GO:0010181", "GO:0036211", "KEGG:K03647", "RefSeq:WP_010869386.1", "SO:0001217", "UniParc:UPI00001304DD", "UniRef:UniRef100_P47472", "UniRef:UniRef50_P47472", "UniRef:UniRef90_P47472" ], "nt": "ATGCATAAAGATATCAAACTAGTTAAGGAAACTGAAATTAGAAAACCAATTGGTTCTCCTTTTATTGTCTATTTTTCATCTATCTCCAACAACACCCACCGTTTTATTGAAAAACTGGGTTTTCAACACAAAAGAATCCCAGTTGATATAACCCAAAGCATTACTGTAAGTAATGAGTATGTTTTAATCTGTCCAACTTATAGTGGTGGGGGTAACCAGGTTGAAGGAGCGGTACCCAAGCAAGTTATCCAGTTTTTAAATAACAAGCATAACAGGGAGTTATGCAGAGGAGTTATTGCATCTGGTAACACTAATTTTGGAGATACTTTTTGTCTTGCAGGAACTGTTATTTCCAAAAAACTAAACGTCCCTTTGTTGTATCAGTTTGAACTTTTGGGAACAAAAAATGATGTAGAACAAACCCAAAAAATAATTGCCAATTTCTTTCAAAACAGCAACTAG", "aa": "MHKDIKLVKETEIRKPIGSPFIVYFSSISNNTHRFIEKLGFQHKRIPVDITQSITVSNEYVLICPTYSGGGNQVEGAVPKQVIQFLNNKHNRELCRGVIASGNTNFGDTFCLAGTVISKKLNVPLLYQFELLGTKNDVEQTQKIIANFFQNSN", "aa_hexdigest": "ee688095cd5a7cee5b63bfb1dc2e917b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001304DD", "ncbi_nrp_id": "WP_010869386.1", "uniref100_id": "UniRef100_P47472", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001304DD", "RefSeq:WP_010869386.1", "UniRef:UniRef100_P47472" ] }, "ips": { "uniref100_id": "UniRef100_P47472", "uniref90_id": "UniRef90_P47472" }, "psc": { "uniref90_id": "UniRef90_P47472", "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "uniref50_id": "UniRef50_P47472", "cog_id": "COG1780", "cog_category": "F", "kegg_orthology_id": "K03647", "go_ids": [ "GO:0009263", "GO:0010181", "GO:0036211" ] }, "pscc": { "uniref50_id": "UniRef50_P47472", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47472" ], "product": "Protein NrdI" }, "genes": [ "nrdI" ], "id": "KKNAAMEMLJ_514", "locus": "GCA000027325_00488" }, { "type": "cds", "sequence": "contig_1", "start": 276647, "stop": 278275, "strand": "+", "frame": 2, "gene": null, "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "nt": "ATGACATCCAAAGAAAAAATCCCTACTTTTAATACTGAAGAAGATGTTGAAAGTTACATTTCTTTTAATGCCCAAGCCAAAATCTATGATGATTTTGCAATCGATTTACAAGCAGTTGAAAGCTATATTCAAGAGCATGTAAAACCCAAAACTAAGGTCTTTCATTCCACCAAAGAACGCCTTGATTTTCTGATTAAGAACGATTATTATGATGAGAAGATCATCAACATGTACAGTTTTGAACAGTTTGAAGAGATCACCCATAAAGCATATTCATACCGCTTTCGTTATGCTAACTTCATGGGAGCATTTAAGTTCTATAATGCCTATGCTTTAAAGACATTTGATGGTAAGTACTACTTGGAAAACTATGAGGATAGGGTGGTGATGAATGTATTGATGTTAGCTAATGGTAACTTCAATAAGGCATTAAAACTCTTAAAACAGATTATCCTTAACCGTTTTCAACCAGCAACCCCTACCTTTCTTAATGCTGGTAGAAAGAAACGTGGTGAATTTGTTTCATGTTACCTGTTAAGGATTGAAGATAACATGGAATCAATAGGTAGAGCGATAACAACTACACTACAACTATCAAAACGTGATGGGGGAGTAGCACTTTTGCTTTCCAACTTACGTGAAGCGGGAGCGCCCATCAAAAAGATAGAAAACCAATCATCAGGGATTATCCCAATTATGAAATTGTTAGAGGACTCTTTTTCCTATTCCAACCAACTTGGACAAAGACAAGGAGCGGGAGCGGTGTATCTCCATTGTCACCATCCTGATGTTATGCAGTTTTTAGATACTAAAAGGGAAAATGCTGATGAGAAGATCAGAATTAAATCACTCTCCTTAGGACTTGTGATTCCAGATATCACCTTCCAATTAGCAAAAAATAACGAGATGATGGCACTTTTCAGTCCATATGATATCTATCAGGAGTATGGTAAGGCTTTATCTGATATCTCAGTAACTGAGATGTATTATGAATTGCTTGAAAACCAACGCATTAAAAAGACCTTTATTAGTGCTAGAAAGTTCTTTCAAACAATTGCTGAACTCCACTTTGAAAGTGGTTATCCCTACATCTTGTTTGATGATACAGTTAACAGGAGAAATGCCCACAAAAACAGGATAGTAATGTCTAACCTTTGCAGTGAAATTGTCCAACCATCTTTACCTTCTGAATTCTATTCAGACCTTACTTTTAAAAAGGTAGGTAGTGATATTAGCTGTAACTTGGGGAGTTTAAATATTGCTAGAGCAATGGAAAGTGGTAGTGAGTTAGCTGAATTGATTCAACTAGCAATTGAATCACTGGATTTAGTGTCAAGGATCAGTAGTTTAGAAACCGCTCCTTCCATTAAAAAAGGTAATTCAGAAAACCATGCGTTGGGATTAGGAGCGATGAACTTACATGGATTTTTAGCAACAAATGCTATCTATTATGATTCAAAGGAAGCGGTTGATTTTACTAACATCTTTTTTTATACAGTAGCATACCATGCGTTTAGTGCTTCCAATAAATTAGCATTGGAACTAGGTAAATTTAAAGACTTTGAAAATACTAAATTTGCTGATGGTAGTTACTTTGATAAGTACACTAAGGTAGCTAGTGACTTTTGA", "aa": "MTSKEKIPTFNTEEDVESYISFNAQAKIYDDFAIDLQAVESYIQEHVKPKTKVFHSTKERLDFLIKNDYYDEKIINMYSFEQFEEITHKAYSYRFRYANFMGAFKFYNAYALKTFDGKYYLENYEDRVVMNVLMLANGNFNKALKLLKQIILNRFQPATPTFLNAGRKKRGEFVSCYLLRIEDNMESIGRAITTTLQLSKRDGGVALLLSNLREAGAPIKKIENQSSGIIPIMKLLEDSFSYSNQLGQRQGAGAVYLHCHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFQLAKNNEMMALFSPYDIYQEYGKALSDISVTEMYYELLENQRIKKTFISARKFFQTIAELHFESGYPYILFDDTVNRRNAHKNRIVMSNLCSEIVQPSLPSEFYSDLTFKKVGSDISCNLGSLNIARAMESGSELAELIQLAIESLDLVSRISSLETAPSIKKGNSENHALGLGAMNLHGFLATNAIYYDSKEAVDFTNIFFYTVAYHAFSASNKLALELGKFKDFENTKFADGSYFDKYTKVASDF", "aa_hexdigest": "631bb5a82b2d3b009845328d30d3072b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001C049A88", "query_cov": 0.9022140221402214, "subject_cov": 0.9683168316831683, "identity": 0.6890000000000001, "score": 687.0, "evalue": 8.28e-244, "valid": false, "product": "ribonucleoside-diphosphate reductase subunit alpha", "ec_ids": [ "1.17.4.1" ], "uniref50_id": "UniRef50_UPI0021AFF10F", "go_ids": [ "GO:0004748", "GO:0005971", "GO:0009265" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021AFF10F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha" }, "genes": [], "id": "KKNAAMEMLJ_515", "locus": "GCA000027325_00489" }, { "type": "cds", "sequence": "contig_1", "start": 278354, "stop": 278812, "strand": "+", "frame": 2, "gene": null, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "nt": "TTGGTAGCAAGTATCCAAAAAGATGGACTTGCAAACTCCCATTTAATGGCTATTGCCCCAACTGGATCTATCTCATATCTCTCTTCATGTACCCCTTCACTTCAACCAGTAGTATCTCCTGTTGAAGTGAGAAAAGAAGGGAAGTTAGGACGGATTTATGTCCCTGCTTATAAGCTTGATAATGATAACTATCAGTACTTTAAAGATGGTGCTTATGAACTGGGCTTTGAACCTATTATTAACATAGTAGCAGCAGCCCAACAACATGTTGATCAAGCAATCTCTTTAACCTTGTTTATGACTGATAAAGCTACCACCAGAGATCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTAGTTCTATCTATTATGTCAGAGTAAGACAAGATGTTTTAAAAGATAGTGAAGATCACACTATTAAAATCAAGGATTGTGAGGTTTGTTCTATCTAA", "aa": "MVASIQKDGLANSHLMAIAPTGSISYLSSCTPSLQPVVSPVEVRKEGKLGRIYVPAYKLDNDNYQYFKDGAYELGFEPIINIVAAAQQHVDQAISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQDVLKDSEDHTIKIKDCEVCSI", "aa_hexdigest": "ed87e14e2ff7b0324ee5d6ca5ad0f03d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A655JDN6", "query_cov": 0.9736842105263158, "subject_cov": 1.0422535211267605, "identity": 0.52, "score": 157.0, "evalue": 6.68e-47, "valid": false, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "uniref50_id": "UniRef50_A0A655JDN6" }, "pscc": { "uniref50_id": "UniRef50_A0A655JDN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "product": "Ribonucleotide-diphosphate reductase subunit alpha" }, "genes": [], "id": "KKNAAMEMLJ_516", "locus": "GCA000027325_00490" }, { "type": "cds", "sequence": "contig_1", "start": 278905, "stop": 279207, "strand": "+", "frame": 1, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02888", "RefSeq:WP_009885769.1", "SO:0001217", "UniParc:UPI0000133D46", "UniRef:UniRef100_P47474", "UniRef:UniRef50_P47474", "UniRef:UniRef90_P47474" ], "nt": "ATGCATGCTATTGTGGTTTGTGGTGCTAAGCAGTATTTAGTCCATGAAAACGAGTCTATTTTTGTTGAAAAATTAGCTGGTAAAGTTGGTCAGGAGATCCAACTTGATAAGGTATTGATGCTTGATGAAAAGATAGGCAAACCTTACCTTGAAAAAGCTAAGGTTGTTTGTGTGATTGAAAAACACGGTTTAAAATCGAAAATTAAACTAATTAAACACATCTCCCAAAAACACCACCTCAAGCGTTATGGCCACCGTCAACCCTACACCAAACTAAAAGTGGTACGCTTTATCCATGATTAA", "aa": "MHAIVVCGAKQYLVHENESIFVEKLAGKVGQEIQLDKVLMLDEKIGKPYLEKAKVVCVIEKHGLKSKIKLIKHISQKHHLKRYGHRQPYTKLKVVRFIHD", "aa_hexdigest": "3e7656c95e5048e12de9eb4e3e4b4a27", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D46", "ncbi_nrp_id": "WP_009885769.1", "uniref100_id": "UniRef100_P47474", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D46", "RefSeq:WP_009885769.1", "UniRef:UniRef100_P47474" ] }, "ips": { "uniref100_id": "UniRef100_P47474", "uniref90_id": "UniRef90_P47474" }, "psc": { "uniref90_id": "UniRef90_P47474", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_P47474", "cog_id": "COG0261", "cog_category": "J", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47474", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47474" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "KKNAAMEMLJ_517", "locus": "GCA000027325_00491" }, { "type": "cds", "sequence": "contig_1", "start": 279200, "stop": 279499, "strand": "+", "frame": 2, "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "db_xrefs": [ "COG:COG2868", "COG:S", "EC:3.4.22.-", "GO:0006508", "GO:0008234", "GO:0042254", "KEGG:K07584", "RefSeq:WP_009885770.1", "SO:0001217", "UniParc:UPI00001394CA", "UniRef:UniRef100_P47475", "UniRef:UniRef50_P47475", "UniRef:UniRef90_P47475" ], "nt": "ATGATTAAGATTAATATCTCCCAAAACTTTCTAGTTGCAAAAGGTCATGCTTTGTTTGCTGAGAAGGGTAAGGACATAGTTTGTGCTGCAATTAGTGGAATTATCTTTGGGGGGGTGGCTTGGTTTGAACCTGATAAGATTGAATTTACTGAAAATAAATTAGTACCTAGTATAGCACTGAAACTCATTGACCCAACCCCTAATGTAGCAGTTGCTTTTAGTGTTATTACAGTACAATTAAAAGCAATAGCCAATTCCTATCCTAATCACATAGTTATCAATGAAGAGAGTTATGAGTAA", "aa": "MIKINISQNFLVAKGHALFAEKGKDIVCAAISGIIFGGVAWFEPDKIEFTENKLVPSIALKLIDPTPNVAVAFSVITVQLKAIANSYPNHIVINEESYE", "aa_hexdigest": "6ec548ab6a67574f06e1d557cff348cd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394CA", "ncbi_nrp_id": "WP_009885770.1", "uniref100_id": "UniRef100_P47475", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394CA", "RefSeq:WP_009885770.1", "UniRef:UniRef100_P47475" ] }, "ips": { "uniref100_id": "UniRef100_P47475", "uniref90_id": "UniRef90_P47475" }, "psc": { "uniref90_id": "UniRef90_P47475", "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "ec_ids": [ "3.4.22.-" ], "uniref50_id": "UniRef50_P47475", "cog_id": "COG2868", "cog_category": "S", "kegg_orthology_id": "K07584", "go_ids": [ "GO:0008234", "GO:0006508", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P47475", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47475" ], "product": "Ribosomal processing cysteine protease Prp" }, "genes": [ "prp" ], "id": "KKNAAMEMLJ_518", "locus": "GCA000027325_00492" }, { "type": "cds", "sequence": "contig_1", "start": 279492, "stop": 279806, "strand": "+", "frame": 3, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "COG:COG0211", "COG:J", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "RefSeq:WP_009885771.1", "SO:0001217", "UniParc:UPI0000133E08", "UniRef:UniRef100_P47476", "UniRef:UniRef50_P05657", "UniRef:UniRef90_P47476" ], "nt": "ATGAGTAAAAACAGTTACTGCTACCAAATTAACTTACAGTTTTTCGCTTCCAAAAAAGGGGTTGGTTCCACTAAGAATGGACGTGATTCACATTCTAAGCGCTTGGGCGCTAAGAAGGCAGATGGTCAGATGATTAGAGTTGGTCAGATTATCTATAGACAAAGGGGAACTAAGATCTTTGCAGGACAAAATGTTGCAATGGGTAGTGATAACACCCTCTTTGCTTTGAGCGATGGCATTGTCCGCTTTCAAAAGTTTGGTAGTAAACAAAGCAAAACCCGGGTTAACATCATCAAACACCAACTAAATGCCTAA", "aa": "MSKNSYCYQINLQFFASKKGVGSTKNGRDSHSKRLGAKKADGQMIRVGQIIYRQRGTKIFAGQNVAMGSDNTLFALSDGIVRFQKFGSKQSKTRVNIIKHQLNA", "aa_hexdigest": "a68e3703ca3015bb7367831e969166d2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133E08", "ncbi_nrp_id": "WP_009885771.1", "uniref100_id": "UniRef100_P47476", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133E08", "RefSeq:WP_009885771.1", "UniRef:UniRef100_P47476" ] }, "ips": { "uniref100_id": "UniRef100_P47476", "uniref90_id": "UniRef90_P47476" }, "psc": { "uniref90_id": "UniRef90_P47476", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_P05657", "cog_id": "COG0211", "cog_category": "J", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P05657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05657" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [ "rpmA" ], "id": "KKNAAMEMLJ_519", "locus": "GCA000027325_00493" }, { "type": "cds", "sequence": "contig_1", "start": 279799, "stop": 280644, "strand": "+", "frame": 1, "gene": "nfo", "product": "putative endonuclease 4", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0003906", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833", "SO:0001217", "UniRef:UniRef50_P47477", "UniRef:UniRef90_P47477" ], "nt": "ATGCCTAAGCTACTGGGTAGCTTTATTAGCTTTAAAGCCCCCAATTACTTTGTTCAAAGTGCTCAGGATGCAATTGCTATTGATGCAACTGCTCTAATGGTATTTTTAGGTCCACCCCATTCTGCTTACCGTGTTCCTTTTAACAAGATGCAGTTTAGTTTGGGCTATGAGTTGTTAAAAACAAAGAATATTAATAGTAATGGTTTGGTTGTTCATGCTCCATATATCATTAACTGTGCATCAAAAGACCCACTAAAACAGCAGAATGCTATCAGTGTTTTAACCAATGAGATTCAGCTTTGTAACTTGGCTGGTGCTCACTATTTAGTTTTGCATCCAGGTTCTGCAGTAGCCCAAACAACCAACGAAGCATTAGATAACCTGGTTAAAGTACTCAATCAAGTTATCAATAAAACCAAAACAACAGTTATTTGCCTTGAAACAATGGCTGGTAAGGGTAATGAGATAGGCAGAGACTTAACTGAGTTGAAATATGTTATTGACAGGATCGTTGATAAAGATAGGATCGGGGTTTGTCTTGATACCTGTCACTTCCATGATAGTGGGATTGACTTTAGTGATCTAACTGGTGTTTTTAACACCATTACAACTAAACTTGGTTTTGAGTTTCTCAAAGTAATCCATTTAAATGAATCTAAAAATAATTGTGGTTCTAAAAAAGATAGGCATGCTAATATCAATGCTGGGATGATTGGTTTTGAGAACTTAATGAAGTTCATTAGTCACCCCCAAATTAAGGATTTACCTATTATCTTAGAAACCCCTTCAACTAGTTTAAACTACCCAACTATTTACCGTGAAGAGATTAGTCAAATCCGCAGCTGA", "aa": "MPKLLGSFISFKAPNYFVQSAQDAIAIDATALMVFLGPPHSAYRVPFNKMQFSLGYELLKTKNINSNGLVVHAPYIINCASKDPLKQQNAISVLTNEIQLCNLAGAHYLVLHPGSAVAQTTNEALDNLVKVLNQVINKTKTTVICLETMAGKGNEIGRDLTELKYVIDRIVDKDRIGVCLDTCHFHDSGIDFSDLTGVFNTITTKLGFEFLKVIHLNESKNNCGSKKDRHANINAGMIGFENLMKFISHPQIKDLPIILETPSTSLNYPTIYREEISQIRS", "aa_hexdigest": "1ff46dd14e955ac826d0954a7552843b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47477", "query_cov": 1.0, "subject_cov": 0.9656357388316151, "identity": 1.0, "score": 560.0, "evalue": 1.4e-201, "valid": true, "gene": "nfo", "product": "Probable endonuclease 4", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_P47477", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0008833", "GO:0003677", "GO:0003906", "GO:0008081", "GO:0008270", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_P47477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47477" ], "product": "Probable endonuclease 4" }, "genes": [ "nfo" ], "id": "KKNAAMEMLJ_520", "locus": "GCA000027325_00494" }, { "type": "cds", "sequence": "contig_1", "start": 280664, "stop": 281086, "strand": "+", "frame": 2, "gene": "fur", "product": "putative protein MG236", "db_xrefs": [ "COG:COG0735", "COG:P", "GO:0000976", "GO:0003700", "GO:0008270", "GO:0045892", "GO:1900376", "RefSeq:WP_009885773.1", "SO:0001217", "UniParc:UPI00001394D0", "UniRef:UniRef100_P47478", "UniRef:UniRef50_P75456", "UniRef:UniRef90_P47478" ], "nt": "ATGCTAACTAGTTATGTGAAGGTATTAGAACAAAACAATCTGCGCTTAACAAAACCGCGGATTGCTTTATTAAAGTGTTTAATTGAACACCAAGATTGGCATAATCTCTCCCAAATTAAAACCCACCTTGATTTAGCCAATCAACCCTCAACACTCGCTTCTATCTACAACAACTTAAGAATCCTAGCTAAACTTAAACTGATTAACATCTTTGTAGATCCAGAGCGGTTTGAAACTTACTATTGCCTGCGCCATGCAGAGCATAACCATATCTATCTTTTTGATGAAGTTAAACAGCAGTTTTTTACCTTACCTTTAACAGATGGCCAAATTAAAACATTGCTTGAAACCCAAAACCATACCAGTAAGGTAAAGCTTAATGATTTTTATATTGTTGCTAGGGGTGAGATAAACAATGATTAA", "aa": "MLTSYVKVLEQNNLRLTKPRIALLKCLIEHQDWHNLSQIKTHLDLANQPSTLASIYNNLRILAKLKLINIFVDPERFETYYCLRHAEHNHIYLFDEVKQQFFTLPLTDGQIKTLLETQNHTSKVKLNDFYIVARGEINND", "aa_hexdigest": "d36eeb029ffe79c931482dba3a03810b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D0", "ncbi_nrp_id": "WP_009885773.1", "uniref100_id": "UniRef100_P47478", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D0", "RefSeq:WP_009885773.1", "UniRef:UniRef100_P47478" ] }, "ips": { "uniref100_id": "UniRef100_P47478", "uniref90_id": "UniRef90_P47478" }, "psc": { "uniref90_id": "UniRef90_P47478", "gene": "Fur", "product": "Uncharacterized protein MG236", "uniref50_id": "UniRef50_P75456", "cog_id": "COG0735", "cog_category": "P", "go_ids": [ "GO:0003700", "GO:0000976", "GO:0008270", "GO:0045892", "GO:1900376" ] }, "pscc": { "uniref50_id": "UniRef50_P75456", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75456" ], "product": "Uncharacterized protein MG236 homolog" }, "genes": [ "fur" ], "id": "KKNAAMEMLJ_521", "locus": "GCA000027325_00495" }, { "type": "cds", "sequence": "contig_1", "start": 281079, "stop": 281963, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG237", "db_xrefs": [ "RefSeq:WP_009885774.1", "SO:0001217", "UniParc:UPI00001394D3", "UniRef:UniRef100_P47479", "UniRef:UniRef50_P75455", "UniRef:UniRef90_P47479" ], "nt": "ATGATTAACAAACCAAACCAATTTTTAAACCACCTTGATGGCTTAAAGCAACATTTTTCTGATTATGATTCACTACAAAAATCCTTTAAGAAATATCTATCAGAAAATCAAACTGAACTTAACAATTTCTTTTTTAACCAGTTTGAAAAGATCATTGTTCTGGTCAAAAAAAAGGAGTTTAAAACTGCTCAAGAGAGGTGTGAAGAGGAGTTAGCTACCCCTTATTTTTCAAAGCCATTGGTTGGTTTTTTCCAATCACTATTACAACTAATTAACCATGATCTCATCGAACAGAAAAACCAACAGTTAGCTAACATGAGTTGTGAAAAAATTGTGGAGATGGTGTTAAGTGATTACCCCAATAAACTTAACTTAATCCACTATTTGTTAGCAAAAGAAGCGAGTTTTGTAAACCCTAACCTTTTACAGCGGATGACTTTTGTGTTAACTGATCTTGAACTGTTAGAGTTAAAGCGTTTCTCTTTTTTTAAAGCCCTTAACCAGATACCTGCTTTTAAAAACCACAAAGTAACATACTTTAACAGCAAACTCAAACAGAAGTTTGTAATAACATTAGGTGAATTTGCTTTCCCCCAAACTGATAAAACCAAACAGTTTTTCCAACAACTAATTAAAAAAGTAAGTCAACTGTTTTTAAAAGAACCTGTTAGTTGTGAATTTGCTTATGAAATTATTGATGCATTACTCGTCAGTTTTTTTCCACTCCATCCTAATTTAGAAGTAAACCACTTAGCTAAAAAGATCCACCAGTATGTTAGTAAGATTGTCATTAATGAAGTTGTTGATCTGAAAGATCCAACCACTAAACTAATTGTTGATACACTTTATGAACAGTTAGATAGAGCAATTGGTGAGGAAAATTAA", "aa": "MINKPNQFLNHLDGLKQHFSDYDSLQKSFKKYLSENQTELNNFFFNQFEKIIVLVKKKEFKTAQERCEEELATPYFSKPLVGFFQSLLQLINHDLIEQKNQQLANMSCEKIVEMVLSDYPNKLNLIHYLLAKEASFVNPNLLQRMTFVLTDLELLELKRFSFFKALNQIPAFKNHKVTYFNSKLKQKFVITLGEFAFPQTDKTKQFFQQLIKKVSQLFLKEPVSCEFAYEIIDALLVSFFPLHPNLEVNHLAKKIHQYVSKIVINEVVDLKDPTTKLIVDTLYEQLDRAIGEEN", "aa_hexdigest": "3ddd0b764b47994e1c3f34780f7355db", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D3", "ncbi_nrp_id": "WP_009885774.1", "uniref100_id": "UniRef100_P47479", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D3", "RefSeq:WP_009885774.1", "UniRef:UniRef100_P47479" ] }, "ips": { "uniref100_id": "UniRef100_P47479", "uniref90_id": "UniRef90_P47479" }, "psc": { "uniref90_id": "UniRef90_P47479", "product": "Uncharacterized protein MG237", "uniref50_id": "UniRef50_P75455" }, "pscc": { "uniref50_id": "UniRef50_P75455", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75455" ], "product": "Uncharacterized protein MG237 homolog" }, "genes": [], "id": "KKNAAMEMLJ_522", "locus": "GCA000027325_00496" }, { "type": "cds", "sequence": "contig_1", "start": 282104, "stop": 283327, "strand": "+", "frame": 2, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005737", "GO:0005854", "GO:0015031", "GO:0043022", "GO:0043335", "GO:0044183", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0061077", "GO:0140839", "GO:0140840", "SO:0001217", "UniRef:UniRef50_P47480", "UniRef:UniRef90_P47480" ], "nt": "ATGGCTAAATCGATAAAAATTAAGGGTTTTCGTCCCGGTAAAATCCCCCCTAATTTAGCCAGTCAGTCGATTAATAAAGCTGAATTAATGCAAAAAAGTGCCCAAAACGTCATGAACAGTATTTATGAATCAGTTCAACAAGAAGAGATCGTTGCTAGTAATGATAATGTCATTGATGATTATCCTACCATTGATTTCAAAACGATCACTGAACAAAACTGTGTACTTTTGTTTTACTTTGATCTGATCCCTAACTTTCAACTCCCTGATTACAAAAAGATAAAAGATTTAACACCACTTACCAAGTTAACTGAAGCTGAATTTAACAACGAAATTGAAAAGCTGGCAAAAACTAAAAGCACAATGGTAGATGTTAGTGATAAAAAACTAGCTAATGGTGATATTGCTATCATTGATTTCACTGGGATAGTTGATAACAAAAAACTAGCATCAGCTTCAGCACAAAACTATGAATTGACAATTGGTTCAAATAGCTTTATTAAGGGTTTTGAAACCGGGTTAATAGCAATGAAAGTTAACCAGAAAAAAACTTTAGCACTAACTTTTCCTAGTGATTATCATGTTAAGGAGTTGCAATCAAAACCAGTTACATTTGAAGTAGTTTTAAAAGCAATTAAAAAACTGGAATTCACCCCAATGGATGAAACTAATTTCAAATCCTTTCTCCCTGAACAATTCCAAAGCTTTACTTCTCTAAAGGCATTTAAGAGTTATTTTCATAAGCTAATGGAAAACAAAAAACAAGAGACAATTCTCCAGGAGAATAACCAAAAAATTCGTCAGTTCTTACTTACTAATACCAAACTTCCTTTTCTTCCAGAAGCGTTAATTAAACTAGAAGCTAACCGCTTGTTAAAGCTCCAGCAAAGCCAAGCTGAACAATATAAAATCCCCTTTGAAAAACTCTTAAGTGCTTCTAATATCACCCTAACAGAGTTACAAGATCGCAACATAAAAGAAGCTAAGGAAAATGTTACCTTTGCTTTGGTAATGAAAAAGATAGCTGACATTGAAAAGATTAAGGTTGATAATAACAAGATTAAAGCTGAAATTGAAAATGTTATTGCTGTTGAATATCCCTTTGCTAGTGATGAAATGAAAAAACAACTCTTTTTTAATATGGAACAACAAAAGGAGTTTGTGGAATCAATTATCATCAACAGATTAACAACAACTAAAATCGTTAGCTATTCAACTCATTAG", "aa": "MAKSIKIKGFRPGKIPPNLASQSINKAELMQKSAQNVMNSIYESVQQEEIVASNDNVIDDYPTIDFKTITEQNCVLLFYFDLIPNFQLPDYKKIKDLTPLTKLTEAEFNNEIEKLAKTKSTMVDVSDKKLANGDIAIIDFTGIVDNKKLASASAQNYELTIGSNSFIKGFETGLIAMKVNQKKTLALTFPSDYHVKELQSKPVTFEVVLKAIKKLEFTPMDETNFKSFLPEQFQSFTSLKAFKSYFHKLMENKKQETILQENNQKIRQFLLTNTKLPFLPEALIKLEANRLLKLQQSQAEQYKIPFEKLLSASNITLTELQDRNIKEAKENVTFALVMKKIADIEKIKVDNNKIKAEIENVIAVEYPFASDEMKKQLFFNMEQQKEFVESIIINRLTTTKIVSYSTH", "aa_hexdigest": "4f0adacbb74f6492382dc9af35ead775", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47480", "query_cov": 1.0, "subject_cov": 0.9166666666666666, "identity": 1.0, "score": 763.0, "evalue": 3.29e-277, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_P47480", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005737", "GO:0005854", "GO:0015031", "GO:0043022", "GO:0043335", "GO:0044183", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0061077", "GO:0140839", "GO:0140840" ] }, "pscc": { "uniref50_id": "UniRef50_P47480", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47480" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "KKNAAMEMLJ_523", "locus": "GCA000027325_00497" }, { "type": "cds", "sequence": "contig_1", "start": 283396, "stop": 284373, "strand": "+", "frame": 1, "gene": null, "product": "LON peptidase substrate-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E" ], "nt": "ATGCCCGTTACGAAGAAAAGTCAGATCTTAGTAGTTAGAGGTCAAGTCATTTTTCCTTTTGTTCCCTTTAGTTTGGATGTTGGCAGGCCCCGTTCGCGTAAGATCATCAAAGCGCTTAAAACTCTGAAAACCAAACGTTTGGTTTTAGTAACCCAAAAGTTTACTGGTGAACAAAACCCTGAGTTTAATGACATCTATCATGTCGGTACACTCTGTGAGATTGATGAGATAGTTGATGTTCCAGGGGTTGATAGTAAAACAGTAGACTACCGTATTAAAGGCAGAGGTTTACAACGGGTTTTAATTGAAAAATTCTCAGATGCAGATATTAATGAAGTTAGTTACCAATTACTTAACTCCACAGTTAAAGATGAAGCTAATGTTGACAGGTTCTTACAGCGAATCTTTCCTGAAAAAGAAGAAATTGAACAGTTAATGGAAGGAGCTGAGAAGTTTTTAGAACTTGAAAACATCAGCAAAACAGTTAATGTTCCTAAGGGTTTAAAGCAACTTGATATTATCACCTTTAAACTGGCTAATCTTGTCCCTAACACTGAAAGTATTAAACAAGCTATCTTAGAGGAAAATGAGATAGCAAACCGATTGGAAAAGATTATCCAAGCAGGGATTGAAGATTTACAGAAGATCCAAGATTATGGTAGATCTAAAAACAAGGAAACTGAGTTTGATAAACTTGACAGTAAAATTACCCGCAAAATTAACGAACAACTCTCAAGACAACAACGTGATTTCTATCTTCGTGAAAAGCTAAGAATTATCCGTGAAGAGATAGGGATTAGTTCCAAAAAAGAGGATGAAGTTGCTAGTATTAGAAAGAAACTGGATGAAAACCCTTACCCTGAAGCCATTAAAAAACGGATTTTAAGTGAACTTGAACACTATGAAAACTCTTCCTCCTCTTCCCAAGAATCAACCTTAACCAAAACTTACATTGATACGCTTTTAAACCTGCCTTGA", "aa": "MPVTKKSQILVVRGQVIFPFVPFSLDVGRPRSRKIIKALKTLKTKRLVLVTQKFTGEQNPEFNDIYHVGTLCEIDEIVDVPGVDSKTVDYRIKGRGLQRVLIEKFSDADINEVSYQLLNSTVKDEANVDRFLQRIFPEKEEIEQLMEGAEKFLELENISKTVNVPKGLKQLDIITFKLANLVPNTESIKQAILEENEIANRLEKIIQAGIEDLQKIQDYGRSKNKETEFDKLDSKITRKINEQLSRQQRDFYLREKLRIIREEIGISSKKEDEVASIRKKLDENPYPEAIKKRILSELEHYENSSSSSQESTLTKTYIDTLLNLP", "aa_hexdigest": "055cd860e4b9ddb0b1d959506f0a4f0f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F21836E", "query_cov": 0.803076923076923, "subject_cov": 1.0, "identity": 0.594, "score": 303.0, "evalue": 1.14e-99, "valid": false, "product": "LON peptidase substrate-binding domain-containing protein", "uniref50_id": "UniRef50_UPI001F21836E" }, "pscc": { "uniref50_id": "UniRef50_UPI001F21836E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E" ], "product": "LON peptidase substrate-binding domain-containing protein" }, "genes": [], "id": "KKNAAMEMLJ_524", "locus": "GCA000027325_00498" }, { "type": "cds", "sequence": "contig_1", "start": 284518, "stop": 285783, "strand": "+", "frame": 1, "gene": "lon", "product": "endopeptidase La", "db_xrefs": [ "COG:COG0466", "COG:O", "EC:3.4.21.53", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565", "SO:0001217", "UniRef:UniRef50_P47481", "UniRef:UniRef90_P47481" ], "nt": "ATGTGTTTAGTAGGTCCTCCTGGGGTTGGTAAATCAAGTCTAGCTAAGTCTATTGCAGAAGCATTAGATAAGAAGTTTGTCAAGATCTCATTAGGGGGAGTACATGATGAATCGGAAATCAGAGGTCACCGTAAAACTTACTTAGGTTCTATGCCAGGAAGGATTTTGAAAGGGATGACCCGTGCTAAGGTAATTAATCCCCTCTTTTTACTTGATGAAATTGATAAGATGACCTCCTCCAACCAAGGTTATCCTTCAGGTGCTTTACTTGAAGTATTAGATCCAGAGTTAAATAATAAGTTTAGTGATAACTATGTTGAAGAAGATTATGATCTTTCTAAAGTAATGTTTATCGCTACTGCAAACTACATAGAAGATATCCCTGAAGCTTTACTTGATAGGATGGAGATAATTGAACTCACTTCCTATACAGAACAAGAGAAGATTGAGATAGCAAAAAACCACTTAATTAAGCGTTGCCTTGAGGATGCTGATCTTAACAGTGAAGAATTGAAGTTCACTGATGAAGCAATCAGCTACATCATTAAGTTTTACACAAGAGAAGCGGGGGTTAGACAATTAGAACGATTAATCCAACAAGTTGTAAGAAAGTACATAGTAGCAATGCAAAAAGATGGCATCAAACAAGAAACGATTGATGTAAACGCTGTTAAAAAATACCTTAAGAAGGAGATCTTTGATCACACTATGCGTGATGAAGTGTCTCTACCTGGAATTGTCAACGGGATGGCATACACCCCAACTGGAGGGGACTTACTTCCCATAGAAGTTACCCATGTTGCTGGTAAAGGAGAGTTGATCTTAACTGGTAATTTAAAGCAAACAATGCGAGAAAGCGCTAATGTTGCTTTAGGCTATGTAAAAGCTAATGCAGAGCGTTTTAACATTAATCCTAGTTTGTTTAAAAAGATTGATATTAACATCCATGTTCCAGGTGGGGGAATTCCTAAGGATGGACCTAGTGCTGGTGCTGCTTTGGTAACTGCAATCATCTCATCATTAACTGGTAAGAAAGTAGATCCTACAGTGGCTATGACAGGAGAGATCACTTTAAGAGGCAAAGTGTTGGTTATTGGTGGGGTGAAAGAAAAAACTATCTCAGCTTACCGCGGTGGGGTTACAACTATCTTTATGCCTGAGAAAAACGAGCGCTATTTAGATGAAGTACCCAAAGAGATAGTAGATAAACTTAACATTATCTTTGTTAAGGAATACAGTGATATCTACAACAAGCTTTTCAGTTAG", "aa": "MCLVGPPGVGKSSLAKSIAEALDKKFVKISLGGVHDESEIRGHRKTYLGSMPGRILKGMTRAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFIATANYIEDIPEALLDRMEIIELTSYTEQEKIEIAKNHLIKRCLEDADLNSEELKFTDEAISYIIKFYTREAGVRQLERLIQQVVRKYIVAMQKDGIKQETIDVNAVKKYLKKEIFDHTMRDEVSLPGIVNGMAYTPTGGDLLPIEVTHVAGKGELILTGNLKQTMRESANVALGYVKANAERFNINPSLFKKIDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPTVAMTGEITLRGKVLVIGGVKEKTISAYRGGVTTIFMPEKNERYLDEVPKEIVDKLNIIFVKEYSDIYNKLFS", "aa_hexdigest": "1a44bb6b2e598a29421683a386fb6ae8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025901479", "query_cov": 0.9857482185273159, "subject_cov": 0.8169291338582677, "identity": 0.537, "score": 468.0, "evalue": 1.55e-159, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A7C6Y0Z4", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "genes": [ "lon" ], "truncated": "5-prime", "pseudogene": { "start": 284218, "stop": 285783, "observations": { "mutation": [ 284371, 284374 ], "stop": [ 284371, 284374 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47481", "query_cov": 1.0, "subject_cov": 0.529559748427673, "identity": 1.0, "score": 819.0, "evalue": 1.17e-293, "gene_start": 1, "gene_end": 421, "reference_start": 375, "reference_end": 795, "reference_sequence": "MPVTKKSQILVVRGQVIFPFVPFSLDVGRPRSRKIIKALKTLKTKRLVLVTQKFTGEQNPEFNDIYHVGTLCEIDEIVDVPGVDSKTVDYRIKGRGLQRVLIEKFSDADINEVSYQLLNSTVKDEANVDRFLQRIFPEKEEIEQLMEGAEKFLELENISKTVNVPKGLKQLDIITFKLANLVPNTESIKQAILEENEIANRLEKIIQAGIEDLQKIQDYGRSKNKETEFDKLDSKITRKINEQLSRQQRDFYLREKLRIIREEIGISSKKEDEVASIRKKLDENPYPEAIKKRILSELEHYENSSSSSQESTLTKTYIDTLLNLPWWQKSKDNSDVKNLIKTLDKNHTGLDKVKERIVEYLAVQLRTQKNKGPIMCLVGPPGVGKSSLAKSIAEALDKKFVKISLGGVHDESEIRGHRKTYLGSMPGRILKGMTRAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFIATANYIEDIPEALLDRMEIIELTSYTEQEKIEIAKNHLIKRCLEDADLNSEELKFTDEAISYIIKFYTREAGVRQLERLIQQVVRKYIVAMQKDGIKQETIDVNAVKKYLKKEIFDHTMRDEVSLPGIVNGMAYTPTGGDLLPIEVTHVAGKGELILTGNLKQTMRESANVALGYVKANAERFNINPSLFKKIDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPTVAMTGEITLRGKVLVIGGVKEKTISAYRGGVTTIFMPEKNERYLDEVPKEIVDKLNIIFVKEYSDIYNKLFS" }, "paralog": false, "identity": 0.9961612284069098, "score": 1009.0, "evalue": 0.0, "description": "Internal stop codon at 284371, 284374. Nonsense mutation around 284371, 284374.", "psc": { "uniref90_id": "UniRef90_P47481", "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_P47481", "cog_id": "COG0466", "cog_category": "O", "go_ids": [ "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47481", "EC:3.4.21.53", "UniRef:UniRef50_P47481", "COG:COG0466", "COG:O", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ] }, "pscc": { "uniref50_id": "UniRef50_P47481", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47481" ], "product": "Lon protease" } }, "id": "KKNAAMEMLJ_525", "locus": "GCA000027325_00499" }, { "type": "cds", "sequence": "contig_1", "start": 285780, "stop": 285998, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTAATGCCATCAAAAACCACACCATCCCACCAAAACAAGTTAGCAAGCTAGATATGATTGTTTATTTAGCAGATAAACTAGAACCTAACCGTAAACAGGAACAGTGGAGTGGGGGAATTGAGATTGATCAACTAGTAAAACTAGCTAAAAGCAACTTAAAACAGGCCTATTTAATTACCTTAAAATATGTCCAAAACCTAGTTAAAGACTAA", "aa": "MIINAIKNHTIPPKQVSKLDMIVYLADKLEPNRKQEQWSGGIEIDQLVKLAKSNLKQAYLITLKYVQNLVKD", "aa_hexdigest": "ecff59f73e4a94e84c6848c30caa51b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8290.6983, "isoelectric_point": 9.477255439758302 }, "id": "KKNAAMEMLJ_526", "locus": "GCA000027325_00500" }, { "type": "cds", "sequence": "contig_1", "start": 286062, "stop": 286463, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGCACGTTTGTTTGTTATGAACGAAAACCTTATCCTTTTAATAAAAAAATAGCAAACCAATTTAATGTTAAATACTTGGCTAAATGCCCACTTGAAATCGCTTCAAGTAAACTTTTAAATCAACCTAGAAAAAAACTGATACCACTGGCAGTTTTAAATTACATCAATACCAATCACCTCTATCTAATTCCCACTTTAAAAGCAATGGTTGATGATAAGAGGTTTCAACACTGTTTAAGGGTTGGTAAACTAGCAAAACAACTAGCAATTGCAAACAAACTAGATGCTAAAAGAGCATTTGTTGCTGGGGCTTATCATGACTTGGCCAAACAACTACCAGTTGATCAGTTAGTTAACATTGCAACTAGTGAACTGAAGATAACTAACTACCCAAGTTGA", "aa": "MCTFVCYERKPYPFNKKIANQFNVKYLAKCPLEIASSKLLNQPRKKLIPLAVLNYINTNHLYLIPTLKAMVDDKRFQHCLRVGKLAKQLAIANKLDAKRAFVAGAYHDLAKQLPVDQLVNIATSELKITNYPS", "aa_hexdigest": "68f963d331e9133c1eeced9e4304f3cb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15161.832799999982, "isoelectric_point": 9.77219829559326 }, "id": "KKNAAMEMLJ_527", "locus": "GCA000027325_00501" }, { "type": "cds", "sequence": "contig_1", "start": 286488, "stop": 286832, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01467.32" ], "nt": "TTGAAGCAAAAAATTATTATCTTTGGTGGTTCTTTTGACCCTATTCACAATGCCCATCTCTACATTGCTAAACATGCAATTAAAAAAATAAAAGCGCAAAAGCTTTTTTTTGTACCAACTTACAATGGTATTTTTAAAAATAATTTTCATGCTAGTAATAAGGATAGGATTGCTATGTTAAAGCTAGCAATTAAATCAGTCAACAATGCTTTAGTTTCCAATTTTGATATTAAAACTAAAAACGCTTTTTCAATTAATACTGTTAACCACTTTAAAAGTTGTTATCCAACTAGTGAAATCTATTTTTTAATAGGGAGTGATAAATTAAATGAACTTGAGAAGTGA", "aa": "MKQKIIIFGGSFDPIHNAHLYIAKHAIKKIKAQKLFFVPTYNGIFKNNFHASNKDRIAMLKLAIKSVNNALVSNFDIKTKNAFSINTVNHFKSCYPTSEIYFLIGSDKLNELEK", "aa_hexdigest": "b29232cddfd57f3f64c02283d46472cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01467.32", "name": "CTP_transf_like", "length": 106, "aa_cov": 0.9298245614035088, "hmm_cov": 0.8867924528301887, "evalue": 1.201517315632671e-16, "score": 56.22679138183594, "start": 7, "stop": 112 } ], "seq_stats": { "molecular_weight": 13020.078299999983, "isoelectric_point": 9.834990501403805 }, "id": "KKNAAMEMLJ_528", "locus": "GCA000027325_00502" }, { "type": "cds", "sequence": "contig_1", "start": 286885, "stop": 287343, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCAAAAGGAGATGGAGATCTATAACCTCTTTACTTTTCAAATTGATCTTGATAAAAAACTGTTGTTTGAAAAATCAAATGATCAGAAGAATTATTCCAAGATTAGAACCCATTATTTCAAGCATAAATTCAAAAATAAAAGTGCTGTTTTTCTCAATAAAAACCTGATTAAAAATAGCTTAAATAAGGTTCTTTTAAACTTTTCTGATTTTGTTAGTGGTGCTGGAATTGATACAGTTTTTAACCAAATCATTGATGAAGATCCTGAGGTTTTAAACTATTTAAAGCAAGTTAAAAAAGATCTTTCCAAAGAAAACAACGCTACATCCCAACTAACCTTTAATGTAACTATTAACCCTAAAAACACCCTCGCCAATTTTTTTGAAGGATTTAATATTTATCTCCATTTTAATGAAGAAAACAATACAGTGATAGGTAGTTTCTCACTACAATGA", "aa": "MIQKEMEIYNLFTFQIDLDKKLLFEKSNDQKNYSKIRTHYFKHKFKNKSAVFLNKNLIKNSLNKVLLNFSDFVSGAGIDTVFNQIIDEDPEVLNYLKQVKKDLSKENNATSQLTFNVTINPKNTLANFFEGFNIYLHFNEENNTVIGSFSLQ", "aa_hexdigest": "e5692d975c48de59b7a506c11193554a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17789.02409999999, "isoelectric_point": 8.669853401184085 }, "id": "KKNAAMEMLJ_529", "locus": "GCA000027325_00503" }, { "type": "cds", "sequence": "contig_1", "start": 287425, "stop": 287529, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGAAATTAGTTTTATGCAGATCATTAATTGTTTTTCCAAAACAAAAATTAATAAACATGGTGAAATTGTTTTAAAAAGTTGTGCTTTTAAACAAAAATGA", "aa": "MHEISFMQIINCFSKTKINKHGEIVLKSCAFKQK", "aa_hexdigest": "3159e2c441e4613814c0f5d0ff1c4a2d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3982.7601000000013, "isoelectric_point": 9.512648582458496 }, "id": "KKNAAMEMLJ_530", "locus": "GCA000027325_00504" }, { "type": "cds", "sequence": "contig_1", "start": 287749, "stop": 288234, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGCTCTTTTTAAACGATTGGTACAACGAAAATCAAGCAATAACAACTGCTGACATTGAAAATGTTAATGAGGTTGAAGACATAGGAAAATTAGTTGAAAAATACAGCACTAATCAACCCCAAAAGTTATCGTTAAATTCAACAGTTTATGTATTACAAACAAAACAAAAACAGTTTTTTTTAAAAAATGATTTCTTTTTTAATAATAACGAAGCAAAGCTGTTTTTTCTAATAACTATGAAGCCCAATGTTTTTGGTCTTGATGATACAGCAATAGCAAATAACTTAAACCTTAAAAAAATTAGTGATTTTTTTAAAGAGATTGATTTTAATGATGAGGATATTCTAAATGACTTTAAACAAGAACAGGAAAAGTTATTGGTTAGAAGAACATTTAACCAACTGTTATTTATGAACAAAAATACTGAAATTCTCAGTGTTGTCAATGACAAGCAAAAGTCAGTAATCCATAACATTGTTTGA", "aa": "MLLFLNDWYNENQAITTADIENVNEVEDIGKLVEKYSTNQPQKLSLNSTVYVLQTKQKQFFLKNDFFFNNNEAKLFFLITMKPNVFGLDDTAIANNLNLKKISDFFKEIDFNDEDILNDFKQEQEKLLVRRTFNQLLFMNKNTEILSVVNDKQKSVIHNIV", "aa_hexdigest": "bd400d7f6bad3266f503ba3a15230cd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18969.3497, "isoelectric_point": 4.9406953811645495 }, "id": "KKNAAMEMLJ_531", "locus": "GCA000027325_00505" }, { "type": "cds", "sequence": "contig_1", "start": 288259, "stop": 288747, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAGCCTTTGATTATTCCAAAGCCTTTGAAAAAAACCGAACGAGTGATCCTTCCTTATTGCGATCTAATTTAACTGTTATCAACCGCTTAAGATACCTCAGTGAATACTTTCAAAATGCCTCACTTAAGTATGATCTACTCTACACTAAGGCCAAACAATATATGCAAATTGATAAATTTATCAACGACATGATTCGCAAGGTAAACCATGAGGATGAAATCTTTGGCAAATTTAAAGAAAGAATTTATTTAAGTTTGGGAATTATTAGTGCGGTAGTGTTTGGCATAGTTGAATTTTTTAACTGTGTATGGACTATCTTAACTGTTAGTCAAGAAGTGGTTGATAAAAGTGTTTTGGATCCAAGGAATATTATCTTTATTAGTATAGGTACTATTTTGGTTTTATTTCTTTTGGTTACTATCTTGGTTTTTATGACAAGAAGACTTTATCTGTTTGAAATTAATAAAAAGCATAAAAATTAG", "aa": "MLKAFDYSKAFEKNRTSDPSLLRSNLTVINRLRYLSEYFQNASLKYDLLYTKAKQYMQIDKFINDMIRKVNHEDEIFGKFKERIYLSLGIISAVVFGIVEFFNCVWTILTVSQEVVDKSVLDPRNIIFISIGTILVLFLLVTILVFMTRRLYLFEINKKHKN", "aa_hexdigest": "5d8f68a7283e5c2c9c91b68ce463d716", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19152.35400000002, "isoelectric_point": 9.492792320251464 }, "id": "KKNAAMEMLJ_532", "locus": "GCA000027325_00506" }, { "type": "cds", "sequence": "contig_1", "start": 288753, "stop": 289433, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCAATAAGTTAAACCTAAGTCATTGTATCTCTTTTTACATCTCAGAAGTTTCAGAAGTTTTCTTTGAAAGTATCAACCAACACCCTAGTCGTGATTTTGTTAATAACATTCTTCAAAAGATTAAAACTACTTTAAGTGAAGAAGAGCTTGAAAAGTTAAACAGTATTGAAGAAGTTACTAAGGATGAGAAGATTGTGATCATGCTCAACCATGTTTTGAAAAAGATTGTTTCTAAAACTGGTAGTTCTAAGTGTGACTTATTTAATGTAATCAAACAAGATAGGTTTAATTCTCCAGTGTATATCCAAAGTATTAATGCTTTTGAAAACAACCTTATTAACAATGAATTTGCTGAAAGAAGGTATGACTATTTGATTGAAGTTAATAAAAATTCATACCTTAAAAAATTTGTCAATTCGATCAGAATTTCCTTCTTCTTAGATTTAAGAGCACAAATTTTGTCAGGTTCATTTACCCTTAATTTAGTTAATAAGTCAATTGAAAAACAAAAAAAGACAGAAATTTTTAAAGATATTTTTGTTAATGCCTTAGTTAAGCATTTTATCTGTAATCAACTTTATCCTATCTCTTTAAATTCCTTTATTTTTGACAGTGAAAATCCTAGCAATAAACTCGCTCTAAAAGAACGGATTAAGCTTTTAAAAACAAACTGA", "aa": "MKFNKLNLSHCISFYISEVSEVFFESINQHPSRDFVNNILQKIKTTLSEEELEKLNSIEEVTKDEKIVIMLNHVLKKIVSKTGSSKCDLFNVIKQDRFNSPVYIQSINAFENNLINNEFAERRYDYLIEVNKNSYLKKFVNSIRISFFLDLRAQILSGSFTLNLVNKSIEKQKKTEIFKDIFVNALVKHFICNQLYPISLNSFIFDSENPSNKLALKERIKLLKTN", "aa_hexdigest": "6e0486ba57b45cb497b553a42890351b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26446.24570000004, "isoelectric_point": 9.084514045715334 }, "id": "KKNAAMEMLJ_533", "locus": "GCA000027325_00507" }, { "type": "cds", "sequence": "contig_1", "start": 289512, "stop": 289730, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATGCAGTGATTAACACTTATTTAATCATGTTAATCTCTGTTGAAGAGTTAAGGGTTTACTTTACAAGTAAAGAACCAGCACTGATTTTAAAGGTATTAGATAAAAAAAACACACTAAGAGAAGATCCTGATCAAAACCCTGAAACTGATTTGTATGAACTAATACAGTTTATAGAACAAAACTACTTAAAAAAAGATAAAAAAACCAGCTGA", "aa": "MFYAVINTYLIMLISVEELRVYFTSKEPALILKVLDKKNTLREDPDQNPETDLYELIQFIEQNYLKKDKKTS", "aa_hexdigest": "4ad88746209a892188341895700ea933", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8602.875899999995, "isoelectric_point": 5.017541694641112 }, "id": "KKNAAMEMLJ_534", "locus": "GCA000027325_00508" }, { "type": "cds", "sequence": "contig_1", "start": 289836, "stop": 289952, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAATTGATAATGATAATTTTGTCTCTACAAAACAAGTATTTAGAAACCAATTAGAATTACAACTTTTGCATGGGATTGTAATTAATCCTGAGAAGTATGGAATTGGCATGTGA", "aa": "MEIDNDNFVSTKQVFRNQLELQLLHGIVINPEKYGIGM", "aa_hexdigest": "253ffa8719db5ada7c9320d8eb02a9e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4405.0166, "isoelectric_point": 4.895849418640137 }, "id": "KKNAAMEMLJ_535", "locus": "GCA000027325_00509" }, { "type": "cds", "sequence": "contig_1", "start": 290001, "stop": 290165, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAATCCCAAAAGTGGTAATGATTTTTATCAGTTTGAAAAGGACATAGATGAAAGCATATGTCAAATTAACAAAAAATATCTAACTTTTATTAGCAGTGATAGTAATACCTTTTTAATAGTTAAAAATGATGATGTAAAAGTTATTTCTAACTATGTATGA", "aa": "MLNPKSGNDFYQFEKDIDESICQINKKYLTFISSDSNTFLIVKNDDVKVISNYV", "aa_hexdigest": "d29a41e46fa67643fa85e62367fc1e55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6283.009700000002, "isoelectric_point": 4.663718986511231 }, "id": "KKNAAMEMLJ_536", "locus": "GCA000027325_00510" }, { "type": "cds", "sequence": "contig_1", "start": 290334, "stop": 290627, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAAAATAGATAGTAAAAGTAATCTTAAAACCTCGATTGAAGAGATGGTAAATAGGTTTAAACATGAGCAGCGAATTAATAAAAAGGACAATGAAAGGGTGTTGATGATTTTTGCTTATATTGCTGCTTTTGTAGTAGGATTTATCAATTTTTTCTCAATGGTCTTTACTATTCTCACTGTAAGTGATCTAAATGCTGGGCTTACTGTACCTAACATCATTGTAATCAGTATCGCTAGTGTTTTAGCTTTTATTTTGATTGTGATTGCTGTTTTATTTCGTTTTAAATGA", "aa": "MQKIDSKSNLKTSIEEMVNRFKHEQRINKKDNERVLMIFAYIAAFVVGFINFFSMVFTILTVSDLNAGLTVPNIIVISIASVLAFILIVIAVLFRFK", "aa_hexdigest": "80a06bf5565933fbf5a52316216e565c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11056.121499999985, "isoelectric_point": 9.773100852966305 }, "id": "KKNAAMEMLJ_537", "locus": "GCA000027325_00511" }, { "type": "cds", "sequence": "contig_1", "start": 290957, "stop": 291328, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG243", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47485", "UniRef:UniRef90_P47485" ], "nt": "ATGATCCATGAGTTGTTTTTATGGATTTTGCTTTCAGTTTTGTTTTTAGTGATTGCTAGTTATTTTTTTATTAACCAAAATAGCAGCGAAGCGCTTAATTTTTTCACTAATTTAGATAAGCCTAATGCAATAGCGATAACAATTAGAACCATAACAATTTTGATTAGTTTTTTACAACTGATTTTTATTGGTTATTTTTGTTTTAGTTCTGAAAAACAAGCTTTACAAGAGATACTGTCAAACACCTTTATGGTTCAAGAAAAACACACTTTAAAATCAAAACCAACTAGCTTAAAAACCAACAATCAACCTGATCCAGCTAATTTACCAGGCGTAATAGCTCTTGATGAAGTGGAAAAACTCATTAATTAA", "aa": "MIHELFLWILLSVLFLVIASYFFINQNSSEALNFFTNLDKPNAIAITIRTITILISFLQLIFIGYFCFSSEKQALQEILSNTFMVQEKHTLKSKPTSLKTNNQPDPANLPGVIALDEVEKLIN", "aa_hexdigest": "b0ea5b1cdebdf48a95766632e6b96074", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 290657, "stop": 291328, "observations": { "mutation": [ 290729, 290849 ], "stop": [ 290729, 290849 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47485", "query_cov": 1.0, "subject_cov": 0.5442477876106194, "identity": 1.0, "score": 228.0, "evalue": 3.28e-74, "gene_start": 1, "gene_end": 123, "reference_start": 104, "reference_end": 226, "reference_sequence": "MQIKVINETNKTVQIFQCAKVKHRALAWLCDVFLLAIVLVVIFLITQAFSDNRFLLFLVLSCSQTILWTVYFIFLPFFWDGKTLFRNLLKIKLFAFDKRFLRIMIHELFLWILLSVLFLVIASYFFINQNSSEALNFFTNLDKPNAIAITIRTITILISFLQLIFIGYFCFSSEKQALQEILSNTFMVQEKHTLKSKPTSLKTNNQPDPANLPGVIALDEVEKLIN" }, "paralog": false, "identity": 0.9910313901345291, "score": 429.0, "evalue": 1.09e-155, "description": "Internal stop codon at 290729, 290849. Nonsense mutation around 290729, 290849.", "psc": { "uniref90_id": "UniRef90_P47485", "product": "Uncharacterized protein MG243", "uniref50_id": "UniRef50_P47485", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47485", "UniRef:UniRef50_P47485", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47485", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47485" ], "product": "Uncharacterized protein MG243" } }, "id": "KKNAAMEMLJ_538", "locus": "GCA000027325_00512" }, { "type": "cds", "sequence": "contig_1", "start": 291334, "stop": 292098, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF13604.12", "PFAM:PF00580.27" ], "nt": "ATGAATGAACAACAAAAACAAGCAATTAGTTGTGGAAAAGGGGTTAATGTTGTTTATTCTGGAGCAGGTACTGGTAAAACAACAATTATTACTAATCGCTTTGCATACTTGGTTAATAAAGAAAAAGTTGATCCTAGCAGAATTTTAGCAATCACCTTTACTAAGAAAGCTGCTAAGGAGATGCAGTTTAGAATCTTGAAACTAATAGATAGTTCTTTAGCTGAGAAAACAAATATCTATACATTTCACAGCTTTTGCAATAAGTTTTTAATTCAAACATTAAAAAAGCGCTTTATCATCGATGATGATATTAGCTATTTCCTAAAGGAATTTTTAGCTGATTCAAAACTCGATATCAACCTAGCGAAACAAATTATTGATAACTTTAAAAATACTTTTGCTGATTTTGAAATAAATAAGTTGGATCAAGATGAAAGGTTAATTAGTTTATGTGAGCATTCACTTCTAAATAAAGATGAAGAATATTCCACTTTAAAAACCCAACTGATTAATGCATTCATTAGCTATGAAAAGAATAAGATATTAAACAATAAACTTGATTTTCATGATCTTTTAATTAAAACTTGTAATTTATTGAGTAATGATAATGATTTACTTAATCAGTGGAGTGAACAGTTTCAGCATATTTTAGTTGATGAATTTCAAGATACCAACCAAATCCAATATGAACTGATCAAGATGTTAGTAACTAAAAATAAAAACTTGTTTTTGGTAGGTGATAATAACCAGATGATTTACCGCTGA", "aa": "MNEQQKQAISCGKGVNVVYSGAGTGKTTIITNRFAYLVNKEKVDPSRILAITFTKKAAKEMQFRILKLIDSSLAEKTNIYTFHSFCNKFLIQTLKKRFIIDDDISYFLKEFLADSKLDINLAKQIIDNFKNTFADFEINKLDQDERLISLCEHSLLNKDEEYSTLKTQLINAFISYEKNKILNNKLDFHDLLIKTCNLLSNDNDLLNQWSEQFQHILVDEFQDTNQIQYELIKMLVTKNKNLFLVGDNNQMIYR", "aa_hexdigest": "734c02e5eed2df0bf894bfc136c2e196", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 263, "aa_cov": 0.9803149606299213, "hmm_cov": 0.9391634980988594, "evalue": 3.324189467804304e-08, "score": 28.653270721435547, "start": 2, "stop": 250 }, { "id": "PF13245.12", "name": "AAA_19", "length": 253, "aa_cov": 0.9803149606299213, "hmm_cov": 0.5177865612648221, "evalue": 5.041377269391878e-27, "score": 89.79872131347656, "start": 5, "stop": 253 }, { "id": "PF13604.12", "name": "AAA_30", "length": 65, "aa_cov": 0.24015748031496062, "hmm_cov": 0.9384615384615385, "evalue": 9.920247667647347e-10, "score": 33.5244255065918, "start": 2, "stop": 62 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 263, "aa_cov": 1.0, "hmm_cov": 0.9657794676806084, "evalue": 3.712116539323527e-77, "score": 254.6881561279297, "start": 1, "stop": 254 } ], "seq_stats": { "molecular_weight": 29636.69570000004, "isoelectric_point": 6.902265739440918 }, "id": "KKNAAMEMLJ_539", "locus": "GCA000027325_00513" }, { "type": "cds", "sequence": "contig_1", "start": 292237, "stop": 292596, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12" ], "nt": "ATGGCCTATGAAAAACAAGTTAAAACTGAAAAAAATCTCTTGTTTTCAACTTTAAATTCTGATAAAAAACCTGTTTATTTTCAAGCTGAATCAGTTGAAAATCAAGCCAATTGGATCTTCAATAAAATCAAAGCACTAAACCAAACAGAAAAGATTAATTTTAAGGATATGGCCATCTTGTTTAGAAAGAACAGAGATATTACTACTATGGTTGAATTGATTGAAGCGGATGGAACAATTCCCTTACCTAAACAAAAGAGTTATTTTAACCAACTAGTAAAACTCCAGCGGGTTTTAATTGCGATTTCAACCAGAACAAATCTTGATATTAAAAGAGCTTTGCAAGCCCTAAAAATTTGA", "aa": "MAYEKQVKTEKNLLFSTLNSDKKPVYFQAESVENQANWIFNKIKALNQTEKINFKDMAILFRKNRDITTMVELIEADGTIPLPKQKSYFNQLVKLQRVLIAISTRTNLDIKRALQALKI", "aa_hexdigest": "427d93c4722c36a82a5ec96882383682", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 72, "aa_cov": 0.6050420168067226, "hmm_cov": 0.9722222222222222, "evalue": 2.4012443494766204e-08, "score": 28.814903259277344, "start": 10, "stop": 81 } ], "seq_stats": { "molecular_weight": 13862.067299999977, "isoelectric_point": 9.800693321228025 }, "id": "KKNAAMEMLJ_540", "locus": "GCA000027325_00514" }, { "type": "cds", "sequence": "contig_1", "start": 292888, "stop": 293445, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12", "PFAM:PF13538.12" ], "nt": "TTGGAATTTGACTCAATTATTGAAAACAGCAGTGATACAATCAATTTGCTAACCGTTCATGCAGCAAAAGGACTTGAGTTTGAAGCTGTATTTATTTATGGCATGAATCAAGGGGATTTTCCCTTATTTTTAAGTCAAAATCAAAATGACGAACAACATTTAATTGATGAATTAAAACTGTTTTATGTTGCTATCACAAGAGCAAAACGTTTTTTGTTTATCACTGCGGTTTTACAAATAAATAACAATTCTATAAAACCATCTAGTTTTTTAAATTACATCAATAAAAGTGAGTATTTAGACATTGCTACTATTAACTATGTATTAGAGCAGGATGATGATTTTTTTGATTCAACTAAAAAAACAGACTATACAAAGAAACTAAGAAAAGAAAGTTTAGACATTATAGTGGGTGATTTAGTTACTAGTAGATACTTTGGAAAAGGAGTTGTAGTTGAAGTGAGAGACAAAGAGGTTTTAGTAGCTTTTAAAGACACACGCTATGGGATGAAATGGATCTTAAAAAACCATAAATCACTAACAAAAGCTTTATATTAA", "aa": "MEFDSIIENSSDTINLLTVHAAKGLEFEAVFIYGMNQGDFPLFLSQNQNDEQHLIDELKLFYVAITRAKRFLFITAVLQINNNSIKPSSFLNYINKSEYLDIATINYVLEQDDDFFDSTKKTDYTKKLRKESLDIIVGDLVTSRYFGKGVVVEVRDKEVLVAFKDTRYGMKWILKNHKSLTKALY", "aa_hexdigest": "08120c03d7ba61bab0a9b782373cf333", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 68, "aa_cov": 0.3675675675675676, "hmm_cov": 0.9705882352941176, "evalue": 8.902918480324488e-25, "score": 82.85357666015625, "start": 10, "stop": 77 }, { "id": "PF13538.12", "name": "UvrD_C_2", "length": 59, "aa_cov": 0.31891891891891894, "hmm_cov": 0.8135593220338984, "evalue": 2.5059503444914017e-11, "score": 38.41843032836914, "start": 16, "stop": 74 } ], "seq_stats": { "molecular_weight": 21431.244100000004, "isoelectric_point": 5.657378196716309 }, "id": "KKNAAMEMLJ_541", "locus": "GCA000027325_00515" }, { "type": "cds", "sequence": "contig_1", "start": 293448, "stop": 293945, "strand": "+", "frame": 3, "gene": "fAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "db_xrefs": [ "COG:COG0212", "COG:H", "EC:6.3.3.2", "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872", "RefSeq:WP_009885782.1", "SO:0001217", "UniParc:UPI00001394EB", "UniRef:UniRef100_P47487", "UniRef:UniRef50_P75430", "UniRef:UniRef90_P47487" ], "nt": "ATGGTTGATAAAAACAGTTTAAGAAAATTAATGCTTCTAAAAAGAGCAGAACTAAATGATCTTGAAAAATCGCATTTAGATCAAAAGATTAACCAAAAATTAATGGCTTTTTTAATAACAAGACCAACAATTAAAAATTTAGCACTTTACATTCCCATTAAAAACGAAGTGGCTTTTTTAGATAACTTTCTAGATTTTCTTAAGTTAAATAAAATTACAAGCTGTTTTCCTAGTATTGTTGATCAATTTAACATGAAGTTTATTGATCAAAATAATAATGAAATTAACCCTAATGATATTGATTGTTTTTTTATCCCTTTATTAGCTTTTAATAAGGCAAACCACAGGATTGGTTTTGGTAAGGGTTATTATGACCGTTATTTATCATTAACTAGCAAAAAACAACTAAAAATAGGGATAGCATATGACTTTCAATATGCAGAATTCACTAATGATCCTTGGGATTATCAATTAGATTTAATTATTTGCAATGGATAA", "aa": "MVDKNSLRKLMLLKRAELNDLEKSHLDQKINQKLMAFLITRPTIKNLALYIPIKNEVAFLDNFLDFLKLNKITSCFPSIVDQFNMKFIDQNNNEINPNDIDCFFIPLLAFNKANHRIGFGKGYYDRYLSLTSKKQLKIGIAYDFQYAEFTNDPWDYQLDLIICNG", "aa_hexdigest": "a9380ce962d3874d46c708a41fac1d43", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394EB", "ncbi_nrp_id": "WP_009885782.1", "uniref100_id": "UniRef100_P47487", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394EB", "RefSeq:WP_009885782.1", "UniRef:UniRef100_P47487" ] }, "ips": { "uniref100_id": "UniRef100_P47487", "uniref90_id": "UniRef90_P47487" }, "psc": { "uniref90_id": "UniRef90_P47487", "gene": "FAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "ec_ids": [ "6.3.3.2" ], "uniref50_id": "UniRef50_P75430", "cog_id": "COG0212", "cog_category": "H", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75430", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75430" ], "product": "5-formyltetrahydrofolate cyclo-ligase" }, "genes": [ "fAU1" ], "id": "KKNAAMEMLJ_542", "locus": "GCA000027325_00516" }, { "type": "cds", "sequence": "contig_1", "start": 293938, "stop": 294093, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13277.11" ], "nt": "ATGGATAACATAAAGGTTCTTTTTTTAGGTGATGTTTATGGCAAAGCTGGTAGAAAGATTATTAGTGATCATCTTCCCATAATTAAAAAAAAGTATCAGTTAAATCTAATTATTGCAAATGCTGAAAACACTACTAATGGTAAGGGTTTAAGTTGA", "aa": "MDNIKVLFLGDVYGKAGRKIISDHLPIIKKKYQLNLIIANAENTTNGKGLS", "aa_hexdigest": "fe570e65d19f9feb85cecc0e6cdc0fc2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13277.11", "name": "YmdB", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 3.6684738963467295e-15, "score": 50.94171142578125, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 5627.5596, "isoelectric_point": 9.698381996154787 }, "id": "KKNAAMEMLJ_543", "locus": "GCA000027325_00517" }, { "type": "cds", "sequence": "contig_1", "start": 294139, "stop": 294783, "strand": "+", "frame": 1, "gene": null, "product": "Putative metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PBN9" ], "nt": "ATGGGTAACCATACCTGGTTTCAAAAGCAAGATTTAGAACTTGTTTTAAACCAAGTTGATGTTATTCGCCCACTTAACTTAATGCAAGATTTTAACTATTTTCAGCTTGGCAAAGGGAGTTATTTATTTAGCTTAAATGGTTTGAAAATAAGGATTACTAACTTGTTAGGAACAAGTATTAACTTACCATTTGCAATAACAAACCCATTTGTGGAATTAAAAAAGTTAGTTTTAACTAAAGATTGTGATCTTCATATCGTTGATTTTCATGCTGAAACAACTAGCGAAAAAAATGCTTTTTGCATGGTTTTTGATGGTTATGTTACTGCTATCTTAGGAACCCACACCCATGTTCCTAGTAATGATTTAAGAATCACTCCTAAAGGAAGTGTTTACATTACTGATGTAGGGATGTGTGGTCCTGGATTTGGTAGTGTTATTGGTGCTAATCCCAAGCAATCAATTAAGTTATTTTGTACAGGTGAAAGACAGTTTTTTGAGGTTTCTAATTGTGGTGCACAACTCAATGGCGTCTTCTTTGAAGTTTGTTCAAAAACCAATCAAGTTGTGAAAATAGAACAGATTAGAATAGTTTTAGATGATGAAAAGTATTTAGCTAATGACTACTTTAATTTAGTTGAGTAA", "aa": "MGNHTWFQKQDLELVLNQVDVIRPLNLMQDFNYFQLGKGSYLFSLNGLKIRITNLLGTSINLPFAITNPFVELKKLVLTKDCDLHIVDFHAETTSEKNAFCMVFDGYVTAILGTHTHVPSNDLRITPKGSVYITDVGMCGPGFGSVIGANPKQSIKLFCTGERQFFEVSNCGAQLNGVFFEVCSKTNQVVKIEQIRIVLDDEKYLANDYFNLVE", "aa_hexdigest": "5ad3d6f535a230a8c00420df0697ca96", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PBN9", "query_cov": 0.8738317757009346, "subject_cov": 0.9540816326530612, "identity": 0.54, "score": 195.0, "evalue": 6.88e-60, "valid": false, "product": "Putative metallophosphoesterase", "ec_ids": [ "3.1.4.16" ], "uniref50_id": "UniRef50_A0A3B0PBN9", "kegg_orthology_id": "K09769" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PBN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PBN9" ], "product": "Putative metallophosphoesterase" }, "genes": [], "id": "KKNAAMEMLJ_544", "locus": "GCA000027325_00518" }, { "type": "cds", "sequence": "contig_1", "start": 294966, "stop": 295151, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACAGCTAACACAAAGAATGTAATAAGACTTGCTAAAGAAACATACTTAGTTATTAAAGTAATCATTATCCAAATTAAAAGACAGATTAAAAATCATCATAAAGATATTGCAAGTAAAGATCCACCAGTAGTAGCAATTGCCTTACCACCCTTAAACTTAAAATACAGCGGAAAGATATGA", "aa": "MITANTKNVIRLAKETYLVIKVIIIQIKRQIKNHHKDIASKDPPVVAIALPPLNLKYSGKI", "aa_hexdigest": "0db4a42fbae561feb4819429db8ec9a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6872.260500000002, "isoelectric_point": 10.237402153015136 }, "id": "KKNAAMEMLJ_545", "locus": "GCA000027325_00519" }, { "type": "cds", "sequence": "contig_1", "start": 295250, "stop": 295486, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAGCTAGTGCAATTGCCATTTTGGTCATTTTTAGCCTAGCTTCTGGTTATCTGTTAGGTTCAATTATTTTTGCTGATATTTTCAGCAAAATACTCAAGAAAAACGTCAGGGAATTTGGTTCAAAAAACCCAGGAGCTACTAACTCAATGCGTGTTTTTGGCTTAAAAATTGGTTTTTTGGTGGCTATTTTTGATGCATTTAAAGGTTTTTTTGCTTTTTTATTAACCTGA", "aa": "MNQASAIAILVIFSLASGYLLGSIIFADIFSKILKKNVREFGSKNPGATNSMRVFGLKIGFLVAIFDAFKGFFAFLLT", "aa_hexdigest": "7718ad0da02956cffe9c736a783e03c6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8469.010999999995, "isoelectric_point": 10.118586921691893 }, "id": "KKNAAMEMLJ_546", "locus": "GCA000027325_00520" }, { "type": "cds", "sequence": "contig_1", "start": 295473, "stop": 296129, "strand": "-", "frame": 1, "gene": "trmK", "product": "Putative tRNA methyltransferase MG248", "db_xrefs": [ "COG:COG2384", "COG:J", "EC:2.1.1.-", "GO:0005737", "GO:0008033", "GO:0032259", "GO:0160105", "KEGG:K06967", "RefSeq:WP_009885786.1", "SO:0001217", "UniParc:UPI00001394FC", "UniRef:UniRef100_P47490", "UniRef:UniRef50_P47490", "UniRef:UniRef90_P47490" ], "nt": "ATGAAAAAAAGGATTAGCACAATTGCTAATTTAGTTCAATCATTTAATCCTAAGCTAGTGTATGACATCGGTTGTGATCATTCCTATCTAACATCCTATCTAATAAAAACTAATCAAAACCTAACCATTGTTAACAGTGATATTAGTAAAAATGCTTTATTAAGTAATTACCAAAAATTTAAAAATAACAATAATATCCACTTCTTTGTTAGTGATGGATTTAATAACCTTCCAGAACTAAATATTAATCCCAAAATAGGGGTAATTGCTGGACTTGGTGGATTGAAAATCATTAACATAATTAGCCAAAAAGAAAACTTTATTAATCGTTTTGTAATCCAACCCCAATCTAATTTAATTGAATTACGTAGTTTTTTAAGTTTAAACAGTTGGGATATTGTTAATGAAACACTAGTTCAGGATCGAGAGTTTATATATCCTATTTTGGTTATTGAAAAACTAAAAAAACCGTTTAAATTAACAAAGGAATTGGTTATTTTAGGACCAAAGCTAATTAACTTTAAAGATAAACACTGTTTAATGAAACACTACCAATGCCTATTGAGAGTTTATCAACCCAAACAAAAACCTAGCTTGATGGATCTTAAGATAATTGAAACATTAAATAAGATAATTACAAGCTATGAATCAAGCTAG", "aa": "MKKRISTIANLVQSFNPKLVYDIGCDHSYLTSYLIKTNQNLTIVNSDISKNALLSNYQKFKNNNNIHFFVSDGFNNLPELNINPKIGVIAGLGGLKIINIISQKENFINRFVIQPQSNLIELRSFLSLNSWDIVNETLVQDREFIYPILVIEKLKKPFKLTKELVILGPKLINFKDKHCLMKHYQCLLRVYQPKQKPSLMDLKIIETLNKIITSYESS", "aa_hexdigest": "3e7f95a754f8ca28e1394692b172531c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394FC", "ncbi_nrp_id": "WP_009885786.1", "uniref100_id": "UniRef100_P47490", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394FC", "RefSeq:WP_009885786.1", "UniRef:UniRef100_P47490" ] }, "ips": { "uniref100_id": "UniRef100_P47490", "uniref90_id": "UniRef90_P47490" }, "psc": { "uniref90_id": "UniRef90_P47490", "gene": "TrmK", "product": "Putative tRNA methyltransferase MG248", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47490", "cog_id": "COG2384", "cog_category": "J", "kegg_orthology_id": "K06967", "go_ids": [ "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_P47490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47490" ], "product": "Putative tRNA methyltransferase MG248" }, "genes": [ "trmK" ], "id": "KKNAAMEMLJ_547", "locus": "GCA000027325_00521" }, { "type": "cds", "sequence": "contig_1", "start": 296113, "stop": 296628, "strand": "-", "frame": 3, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFD7AF2", "UniRef:UniRef90_UPI001EFD7AF2" ], "nt": "ATGGTAGAAACCATTAACCGCTTAGCTAAAGCAGAACGGGCTTTGTATCAAGAGTTAGGGCGAGAACCTACTGATGAGGAGTTAGCTGAAAAGATGGGAGGACAAGCTGAAGGATTTAATGTTAAAAAGATTGCTGAAATTAAACGGTTAAGTTTAGATCCAGTTTCGCTTGATAAAACAGTTGGACATGATGAAGAGTCCCAGTTTGGTGATTTTGTTAAAGACACAGACGCTCAAACTCCTGACGAGTTTACCGAAAGCCGTTCAAATTCAGAAAAAATTGATGAATTGTTGAACAATAATCTTTCTGAACAAGAAGAGTTAATTGTTAGAATGCGGATTGGCATGCCCCCTTACAATGAACCTAAAACACTTGATGAAGTAGGTCAAAAGATTTTGATCCCTAGAGAGAAGATCAGACAAATTGAAAACAAAGCAATTAGAAAATTGAGACATGCAGTTAGAAACAATCCTATTAGTATGTCATTTCTAAGAATTAATGAAAAAAAGGATTAG", "aa": "MVETINRLAKAERALYQELGREPTDEELAEKMGGQAEGFNVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVKDTDAQTPDEFTESRSNSEKIDELLNNNLSEQEELIVRMRIGMPPYNEPKTLDEVGQKILIPREKIRQIENKAIRKLRHAVRNNPISMSFLRINEKKD", "aa_hexdigest": "becc7dd6d586894aa9b67a1b80d21d62", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 296113, "stop": 296928, "observations": { "mutation": [ 296697, 296733 ], "stop": [ 296697, 296733 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFD7AF2", "query_cov": 1.0, "subject_cov": 0.5245398773006135, "identity": 0.9009999999999999, "score": 281.0, "evalue": 1.27e-92, "gene_start": 1, "gene_end": 171, "reference_start": 156, "reference_end": 326, "reference_sequence": "MEEHIHEFRANQDLSIIDEDIEELTTKNISNRDKVDDNVRFFLGSLDSSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFNWSLGNKFSTYATWWIKQAITRAIADQARTVRIPVHMVETINRLAKAERALNQELGREPTAEELAEKMGGQAEGFTVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVRDTDAQMPDEFTESRSNYEKIDELLNNCLSEQEELIVRMRIGMPPYNETKTLDEVSQKIKIPREKIRQIETKAIRKLRQAVRNNHMSLSFMRGNEKKD" }, "paralog": false, "identity": 0.9298892988929889, "score": 478.0, "evalue": 5.95e-173, "description": "Internal stop codon at 296697, 296733. Nonsense mutation around 296697, 296733.", "psc": { "uniref90_id": "UniRef90_UPI001EFD7AF2", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_UPI001EFD7AF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFD7AF2", "UniRef:UniRef50_UPI001EFD7AF2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFD7AF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFD7AF2" ], "product": "RNA polymerase sigma factor" } }, "id": "KKNAAMEMLJ_548", "locus": "GCA000027325_00522" }, { "type": "cds", "sequence": "contig_1", "start": 296731, "stop": 297135, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04542.20" ], "nt": "TTGGATGAACTACGTGATAAGGGGATTCAACTCCAACACGATGTTGAAGAGCACATCCATGAATTTCGTGCTAACCAAGACCTTTCAATTATTGATGAAGATATTGAAGAGTTAACAAGTAAGAACATCTCTAACCGTGATAAGGTTGATGATAATGTTAGGTTCTTTTTAGGATCACTTGACTTTTCTAAAATGTTAGATTTTGAATCTGAACAGCGGATTGCCAAGGTTTTAAATAGTACTGATGAAGAGTCACGTAAGTATGCAATTAATCAGTTGGTTACTTCAAACTTAAGACTAGTTGTTTCTATTGCCAAAAAACACCTAGAAAGAGGGTTGGATTTTAATGATTTAATTCAAGAGGGTAATTTGGGGCTTTTAAAAGCTATTTCCAAATTTAACTGA", "aa": "MDELRDKGIQLQHDVEEHIHEFRANQDLSIIDEDIEELTSKNISNRDKVDDNVRFFLGSLDFSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFN", "aa_hexdigest": "0980eb09e85c9b0dd63841bd4f602a37", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04542.20", "name": "Sigma70_r2", "length": 42, "aa_cov": 0.31343283582089554, "hmm_cov": 1.0, "evalue": 7.822898482199519e-13, "score": 43.18305969238281, "start": 93, "stop": 134 } ], "seq_stats": { "molecular_weight": 15517.207499999988, "isoelectric_point": 4.886641502380371 }, "id": "KKNAAMEMLJ_549", "locus": "GCA000027325_00523" }, { "type": "cds", "sequence": "contig_1", "start": 297241, "stop": 297606, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCACTGACAAAAAAACGCTAGGCGAAAAACCCAATTCAACCAAACCAGAACTATCTGAAGAATTAATTGCTGAACTTAAAAAACAGCGTATTCTTGAAAAGAATCGTCCTTACAAAAAGATGATTTATGTTGACAATAAAGTGCAACGCAAACACCGTCATGAAAACATCGCTTTTCTCAAAACCCTTCATGAAAATAAGGAGAGTGATGTTCCTAAAAAAAGAAGGGGTAGAAAACCTAAACACGCTCCTTTAAAAGAAAAAAATAATCTGAAGTTATTTGATATCTTAGAAGGATCGTTAAAAAGCCACATTGAAAATGATGACACCAACACAGTCATCAACCTTCTAACAGAAGCTTGA", "aa": "MSTDKKTLGEKPNSTKPELSEELIAELKKQRILEKNRPYKKMIYVDNKVQRKHRHENIAFLKTLHENKESDVPKKRRGRKPKHAPLKEKNNLKLFDILEGSLKSHIENDDTNTVINLLTEA", "aa_hexdigest": "c31079dcb7bfcc478a226fe210da7c25", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14179.188199999977, "isoelectric_point": 9.724169349670408 }, "id": "KKNAAMEMLJ_550", "locus": "GCA000027325_00524" }, { "type": "cds", "sequence": "contig_1", "start": 297651, "stop": 297785, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAATTAAAGATATTTTTTTAGATGAATTAACTTGTTATCAAGCTAATGATTTTCAAAACTATCTAAAGACCTTTCAAACGTTATTAAAACAACAAAAGCAGCGCTTAAAAAATTTAAAGTTAACGCTATAA", "aa": "MEIKDIFLDELTCYQANDFQNYLKTFQTLLKQQKQRLKNLKLTL", "aa_hexdigest": "dccd2730aebfe8c31a4b258b9260da37", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5380.241800000001, "isoelectric_point": 8.889303779602052 }, "id": "KKNAAMEMLJ_551", "locus": "GCA000027325_00525" }, { "type": "cds", "sequence": "contig_1", "start": 297843, "stop": 297968, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGAATTAAAACAATCCCATCTAGATGAAGTTTTTAAGGAATGTAACTTTAATTTGTTTGATGTTGAACTTTTTATTGAAAAAGCAAGGATCTATTGGAGTGAAAATCAAACTGCTAACTGA", "aa": "MVELKQSHLDEVFKECNFNLFDVELFIEKARIYWSENQTAN", "aa_hexdigest": "9a52b9846b11b0a8189fffd7a06df2f6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4979.554900000001, "isoelectric_point": 4.591988182067872 }, "id": "KKNAAMEMLJ_552", "locus": "GCA000027325_00526" }, { "type": "cds", "sequence": "contig_1", "start": 298011, "stop": 298211, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAAACTACTTGAATATAGTGATGAAAAAACTCTGTATGAAACAGTTTTAAAACACAAAGAAAAACTTGTATCTAAGTTTGATAACAACCGTTTTTACATAAATACTTCAGGCCATGCTCAACCACCACAAGAATTGCAAAAAACCACTGCAGCACTAGTGCAAACAGCTTTTGAAGAAGCAGTTAATGAGTTGTGA", "aa": "MVKLLEYSDEKTLYETVLKHKEKLVSKFDNNRFYINTSGHAQPPQELQKTTAALVQTAFEEAVNEL", "aa_hexdigest": "6c3fd154940092a323ffeee1993b7cce", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7628.5366, "isoelectric_point": 5.585192680358887 }, "id": "KKNAAMEMLJ_553", "locus": "GCA000027325_00527" }, { "type": "cds", "sequence": "contig_1", "start": 298434, "stop": 299063, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08275.17", "PFAM:PF13362.12", "PFAM:PF13662.12" ], "nt": "ATGAATTATTTAGTTGAAAAAAGAAAGCTTAATAAAACATTAATTGAACAGTTTCAGCTAGGACTTGCTTTTCACAATGAAGATAAGTATCTATGTGAAAGTATGGAAAGATACCCCTTCATTAATCCAAAGATAAAACCGAGTGAATTGTATCTTTTTTCAAAAACTAACCAGCAAGGTCTTGGCTTTTTTGACTTTAATACCAAAAAAGCTACCTTTCAAAACCAGATTATGATCCCTATCCATGACTTTAATGGTAACCCGGTTGGTTTTTCAGCAAGAAGTGTTGATAACATCAACAAACTGAAATATAAAAATAGTGCTGATCACGAATTTTTTAAAAAAGGGGAGCTGTTATTTAACTTTCACAGGTTAAATAAAAACCTCAATCAACTCTTTATTGTGGAAGGTTATTTTGATGTTTTTACACTAACAAACTCCAAGTTTGAAGCTGTTGCATTAATGGGATTAGCATTAAATGATGTGCAAATTAAAGCCATTAAAGCTCACTTTAAGGAGTTACAAACCTTAGTTTTAGCACTTGATAATGATGCTAGTGGTCAAAATGCTGTGTTTAGCTTAATTGAAAAACTTAATAACAACAATTTTATTGTGGAAATTGTTCAGTGA", "aa": "MNYLVEKRKLNKTLIEQFQLGLAFHNEDKYLCESMERYPFINPKIKPSELYLFSKTNQQGLGFFDFNTKKATFQNQIMIPIHDFNGNPVGFSARSVDNINKLKYKNSADHEFFKKGELLFNFHRLNKNLNQLFIVEGYFDVFTLTNSKFEAVALMGLALNDVQIKAIKAHFKELQTLVLALDNDASGQNAVFSLIEKLNNNNFIVEIVQ", "aa_hexdigest": "4a2f6bb18690df8547ab732aa2c055b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08275.17", "name": "DNAG_N", "length": 123, "aa_cov": 0.5885167464114832, "hmm_cov": 0.9024390243902439, "evalue": 1.0236928167899873e-22, "score": 75.69379425048828, "start": 2, "stop": 124 }, { "id": "PF13362.12", "name": "Toprim_3", "length": 81, "aa_cov": 0.3684210526315789, "hmm_cov": 0.9382716049382716, "evalue": 3.178363003108194e-08, "score": 29.07499885559082, "start": 132, "stop": 208 }, { "id": "PF13662.12", "name": "Toprim_4", "length": 81, "aa_cov": 0.36363636363636365, "hmm_cov": 1.0, "evalue": 4.0188946855856147e-13, "score": 44.54798126220703, "start": 131, "stop": 206 } ], "seq_stats": { "molecular_weight": 24185.49400000001, "isoelectric_point": 8.503782844543458 }, "id": "KKNAAMEMLJ_554", "locus": "GCA000027325_00528" }, { "type": "cds", "sequence": "contig_1", "start": 299232, "stop": 299474, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01807.25" ], "nt": "ATGGTCAATAAAAGCAACAGTTTAGATGAACTTTTAAAGCAGATTAAAATTACTGAAATTATTCAACACTACGGGGTTAAAATCCAAACTAAGGGTAATAGTCTACTTGCTTTATGTCCTTTTCATGATGATAAAAATCCTTCTATGTCCATTTCCAGTTCTAAAAACATCTTTAAGTGTTGGGCTTGTAATGCAGCTGGCAACGGAATAGCGTTTATCCAAAAGCATGACCAGTTAGATTGA", "aa": "MVNKSNSLDELLKQIKITEIIQHYGVKIQTKGNSLLALCPFHDDKNPSMSISSSKNIFKCWACNAAGNGIAFIQKHDQLD", "aa_hexdigest": "7ae53ff976d465a91306833323e38d78", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01807.25", "name": "Zn_ribbon_DnaG", "length": 76, "aa_cov": 0.95, "hmm_cov": 0.9868421052631579, "evalue": 5.7553232611374046e-27, "score": 88.54666137695312, "start": 5, "stop": 80 } ], "seq_stats": { "molecular_weight": 8904.173199999994, "isoelectric_point": 8.50629711151123 }, "id": "KKNAAMEMLJ_555", "locus": "GCA000027325_00529" }, { "type": "cds", "sequence": "contig_1", "start": 299485, "stop": 299658, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "GTGTGCTTTGAGACAACTGGTAGTATTGGTAAAAGGTATCGAAAAGCAGATGCAATTGGAATAAAGTATGCAGTCACTTTTGACTTTGAAAGTTTAGAAGATAATGCAGTTACCATCAGAGATAGAGATACTTTAGTTCAACAGCGAATTGCTATCAAAGAATTACCACAATGA", "aa": "MCFETTGSIGKRYRKADAIGIKYAVTFDFESLEDNAVTIRDRDTLVQQRIAIKELPQ", "aa_hexdigest": "1f878184f893ddd4ec01356c38fbc426", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_F2R163", "query_cov": 0.9649122807017544, "subject_cov": 0.8333333333333334, "identity": 0.509, "score": 68.2, "evalue": 5.19e-14, "valid": false, "product": "Glycyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A8T5UJC6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 52, "aa_cov": 0.9122807017543859, "hmm_cov": 1.0, "evalue": 4.918706135939206e-16, "score": 53.81874465942383, "start": 5, "stop": 56 } ], "seq_stats": { "molecular_weight": 6523.367, "isoelectric_point": 6.138861274719238 }, "id": "KKNAAMEMLJ_556", "locus": "GCA000027325_00530" }, { "type": "cds", "sequence": "contig_1", "start": 299662, "stop": 299970, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAACAGGGGTGATTTTGATCTAAAACAACACCAAGAGTTTTCAAAAAAGAGCATGAGTTTTTTTGATAGCGAATTAAACCAACATTTCTTACCTTTCATAATCGAACCTGCGGTTGGCATTGAACGGTTATTTTATGCACTAATTGTCAGTAGTTATAGGAGAGAAATTATTAATGAGGAAGAACGGGAAGTATTGAGTTTACCATTTGACTTATGTCCTGAACAAATTATTGTTTTACCACTTGTAAATAAACTTAAAAAAGAAGCATTTTCTGTATTTGAAACGCTAGCAAAAACAAGGTGA", "aa": "MANRGDFDLKQHQEFSKKSMSFFDSELNQHFLPFIIEPAVGIERLFYALIVSSYRREIINEEEREVLSLPFDLCPEQIIVLPLVNKLKKEAFSVFETLAKTR", "aa_hexdigest": "563bb330efc747edeb11aa1c89f3d862", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11980.710599999986, "isoelectric_point": 5.387790870666504 }, "id": "KKNAAMEMLJ_557", "locus": "GCA000027325_00531" }, { "type": "cds", "sequence": "contig_1", "start": 299974, "stop": 300411, "strand": "-", "frame": 3, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "nt": "GTGAGGGATTTTAACTTACTTTTTCAAACTGAAATCGGGGTTGTAAACAACGATAAACGCCTTGTTTTTCTCCGTCCTGAGACTGCTCAAGGTAGCTTTATTAACTTTAAAAATATCTTGCAAGCTAAGAAGCGTAATTTACCTTTTGCTATTGCCCAGTTTGGTAAAAGCTTTCGTAATGAAATCACCCCAGGTAACTTCTTGTTTAGAACTAGAGAGTTTGAACAGTTTGAAATTGAGTGGTTTTGTAAACCTGATGATGCAAATTCGCTGTTTGAAAAACAATTAATAATGGTAGAACAGTTTCTACAAACAGTGTTAAAAATTAACCCAGAATTGTTAAAAAAACATGAATATGATCAATCAGAATTGGCTCATTATGCCAAAAAAACTACTGACTTTTTGTTTAATTTTCCCCACGGATTAAAGGAGTTATGA", "aa": "MRDFNLLFQTEIGVVNNDKRLVFLRPETAQGSFINFKNILQAKKRNLPFAIAQFGKSFRNEITPGNFLFRTREFEQFEIEWFCKPDDANSLFEKQLIMVEQFLQTVLKINPELLKKHEYDQSELAHYAKKTTDFLFNFPHGLKEL", "aa_hexdigest": "4c2afbe9d13e754f2886f84a1fdbfdf8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0017CB9A86", "query_cov": 0.8137931034482758, "subject_cov": 0.8489208633093526, "identity": 0.534, "score": 137.0, "evalue": 5.23e-39, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0017CB9A86" }, "pscc": { "uniref50_id": "UniRef50_UPI0017CB9A86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "KKNAAMEMLJ_558", "locus": "GCA000027325_00532" }, { "type": "cds", "sequence": "contig_1", "start": 300577, "stop": 300804, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGTTTACAACCAAGAAGTTTATGTTCAGTTTCTCAAACAACATGGTTTTGTATTTCAGAGTAGTGAAATTTACAACGGTTTAAACAATAGTTGGGATTTTGGTCCATTAGGTGCAGTTTTAAAACAACAAATCAAACAAGCTTTATATAACTTTTTTATTAAAAATAAAGCTGATGTTCTTTTAGTTGAAACCCCTATTATTCTCAGCGAATTGGTTTGA", "aa": "MAKVYNQEVYVQFLKQHGFVFQSSEIYNGLNNSWDFGPLGAVLKQQIKQALYNFFIKNKADVLLVETPIILSELV", "aa_hexdigest": "99c1362b2bb61d9d81f7f6d847e552ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8638.8954, "isoelectric_point": 6.534346961975097 }, "id": "KKNAAMEMLJ_559", "locus": "GCA000027325_00533" }, { "type": "cds", "sequence": "contig_1", "start": 301043, "stop": 301348, "strand": "-", "frame": 2, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "EC:2.1.1.-", "GO:0003723", "GO:0005737", "GO:0006396", "GO:0008173", "GO:0032259", "SO:0001217", "UniRef:UniRef50_P47494", "UniRef:UniRef90_P47494" ], "nt": "TTGGTAGCAGTTTTAGATACTAACCAACTCTTAATTCCACTTGATCAACTGGTTAAAGTAGTTGAAAATAAGAAGTGCAGTACACTTGTGATGTTAGATGAGATCCAAGACCCTTATAATTTTGGTGCTATACTAAGAACTTGTTTGGCTAGTGAGGTTGATGGAATTATCTTTAAAAAGAATAACCAAGTCCCTATCAACAATACAGTCATGAAAACTAGTATGGGTAGTGTTTTTTATCAAAACTTAGTGCAAGTAGCTAACTTAAGTTATACGATCACTAAGCTAAAAGAAATAGGATTTTGA", "aa": "MVAVLDTNQLLIPLDQLVKVVENKKCSTLVMLDEIQDPYNFGAILRTCLASEVDGIIFKKNNQVPINNTVMKTSMGSVFYQNLVQVANLSYTITKLKEIGF", "aa_hexdigest": "0ab4095d911ecfd522f47747da48ebf0", "start_type": "TTG", "rbs_motif": null, "genes": [ "rlmB" ], "truncated": "both", "pseudogene": { "start": 300824, "stop": 301552, "observations": { "mutation": [ 300835, 301015, 301045, 301387 ], "stop": [ 300835, 301015, 301045, 301387 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47494", "query_cov": 1.0, "subject_cov": 0.41735537190082644, "identity": 0.99, "score": 194.0, "evalue": 7.3e-61, "gene_start": 1, "gene_end": 101, "reference_start": 69, "reference_end": 169, "reference_sequence": "MKKPRQQSCLFGVKAFEEAINNQVHIKLVNISIRHKKLIPLIEAKKINFQIHSTNWFNNQYRDINHQELVAVLDTNQLLIPLDQLVKVVENKKCSTLVMLDEIQDPYNFGAILRTCLASEVDGIIFKKNNQVPINNTVMKTSMGSVFYQNLVQVANLSYTITKLKEIGFWTVVSTLDPIWKPIDYRKVDFAKKILIVGNEDRGVNQLITKNADCRIKIPMNNKINSLNVSVALGIILFAWKN" }, "paralog": false, "identity": 0.9834710743801653, "score": 462.0, "evalue": 6.63e-169, "description": "Internal stop codon at 300835, 301015, 301045, 301387. Nonsense mutation around 300835, 301015, 301045, 301387.", "psc": { "uniref90_id": "UniRef90_P47494", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47494", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005737", "GO:0006396", "GO:0008173", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47494", "EC:2.1.1.-", "UniRef:UniRef50_P47494", "COG:COG0566", "COG:J", "GO:0003723", "GO:0005737", "GO:0006396", "GO:0008173", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_P47494", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47494" ], "product": "Uncharacterized tRNA/rRNA methyltransferase MG252" } }, "id": "KKNAAMEMLJ_560", "locus": "GCA000027325_00534" }, { "type": "cds", "sequence": "contig_1", "start": 301385, "stop": 301552, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACCACGCCAACAAAGTTGCTTATTTGGTGTTAAAGCCTTTGAGGAAGCGATTAATAACCAAGTTCACATTAAATTGGTAAACATTTCCATTCGTCACAAAAAACTAATACCTTTAATTGAAGCTAAAAAAATTAATTTTCAAATCCATTCAACTAACTGA", "aa": "MKKPRQQSCLFGVKAFEEAINNQVHIKLVNISIRHKKLIPLIEAKKINFQIHSTN", "aa_hexdigest": "81f499d59e3fd886272d7cc121b78df0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6395.550499999998, "isoelectric_point": 10.227280616760254 }, "id": "KKNAAMEMLJ_561", "locus": "GCA000027325_00535" }, { "type": "cds", "sequence": "contig_1", "start": 301894, "stop": 302154, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01406.26" ], "nt": "ATGGCTTTATATAACCAGCCCATTACCAAACATTGGATGCATATTGGTCATTTGATGATTGAAAACCAAAAGATGTCAAAGTCATTGCAGAACTTCTTGTTAGCAGTTGATTTTCTTAACTTTCATGATTTTCGTGTTTTGCGTTGGATCTTTTACCAAAAACACTATTTGCATCCTATTGATCTAAACCAATCATTGATTGAAAAAGCTAATAATGATATTCAAAGGATTGCAAAAACACTTAATGTTGCTAGAACCTGA", "aa": "MALYNQPITKHWMHIGHLMIENQKMSKSLQNFLLAVDFLNFHDFRVLRWIFYQKHYLHPIDLNQSLIEKANNDIQRIAKTLNVART", "aa_hexdigest": "9fbfdc96d5f93acce165d133e66bd98f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 70, "aa_cov": 0.813953488372093, "hmm_cov": 1.0, "evalue": 5.674728774933788e-24, "score": 80.05188751220703, "start": 2, "stop": 71 } ], "seq_stats": { "molecular_weight": 10375.995899999987, "isoelectric_point": 9.695351982116701 }, "id": "KKNAAMEMLJ_562", "locus": "GCA000027325_00536" }, { "type": "cds", "sequence": "contig_1", "start": 302326, "stop": 302865, "strand": "-", "frame": 3, "gene": "cysS", "product": "cysteine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.16", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423", "SO:0001217", "UniRef:UniRef50_UPI001F010120", "UniRef:UniRef90_UPI001F010120" ], "nt": "ATGAAGCAATTTGAACATAGGTTTATGATTGTTGACAGTGTTAGTCAAAAACCAACAACACTAGTTCAAAAAACCATTAACATTTATCTCTGTGGACCCACAGTTTATAACGATTTGCACTTAGGCAACACCAGACCATTAATTGTTTTTGATGTTTTAAATAGAGTTTTAAAAAAGGCTAAATATACCGTTAATTTTGTTCAAAACATCACTGATATTGACGATAAGATCATCAAGATTGCTCAACAACAAGAAGTAAGCGAATCAGTTGTTACAAAACAACAAATCACTGCTTACAAATCACTTTTAAAAAAACTAAATATTCTGCCTATTAAACATATTCAAATCACTGAAAAAATCGATAAAATCCCTGACTATATTGATCAATTAGTAAATCAAAACCATGCTTATGTTTCAACTCAAAACAACGTTTATTTTGCAGTTAATTCACTAAAGCAATATGGTTATCTAGCTAACCGAATGGTGCATTTAGAAGAAACTGATACTGATAAAAAGAACAAATTGGATTTTGTACTTTGA", "aa": "MKQFEHRFMIVDSVSQKPTTLVQKTINIYLCGPTVYNDLHLGNTRPLIVFDVLNRVLKKAKYTVNFVQNITDIDDKIIKIAQQQEVSESVVTKQQITAYKSLLKKLNILPIKHIQITEKIDKIPDYIDQLVNQNHAYVSTQNNVYFAVNSLKQYGYLANRMVHLEETDTDKKNKLDFVL", "aa_hexdigest": "ebf4ad70eb3ca52b5351bb899bda96ef", "start_type": "ATG", "rbs_motif": null, "genes": [ "cysS" ], "truncated": "5-prime", "pseudogene": { "start": 302036, "stop": 302865, "observations": { "deletion": [ 302085 ], "mutation": [ 302268, 302301, 302328 ], "stop": [ 302046, 302268, 302301, 302328 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F010120", "query_cov": 1.0, "subject_cov": 0.6485507246376812, "identity": 1.0, "score": 350.0, "evalue": 9.99e-121, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MKQFEHRFMIVDSVSQKPTTLVQKTINIYLCGPTVYNDLHLGNTRPLIVFDVLNRVLKKAKYTVNFVQNITDIDDKIIKIAQQQEVSESVVTKQQITAYKSLLKKLNILPIKHIQITEKIDKIPDYIDQLVNQNHAYVSTQNNVYFAVNSLKQYGYLANRMVHLEETDTDKKNKLDFVLWKITTAGIKWNSKWGLGRPGWHVECAFLIDYCFKNELTIHGGGVDLKFPHHENENALHMALYNQPITKHWMHIGHLMIENQKDVKVIAELLVSSWFS" }, "paralog": false, "identity": 0.9855072463768116, "score": 540.0, "evalue": 8.26e-198, "description": "Internal stop codon at 302046, 302268, 302301, 302328. Frameshift due to deletion around 302085. Nonsense mutation around 302268, 302301, 302328.", "psc": { "uniref90_id": "UniRef90_UPI001F010120", "gene": "cysS", "product": "cysteine--tRNA ligase", "ec_ids": [ "6.1.1.16" ], "uniref50_id": "UniRef50_UPI001F010120", "go_ids": [ "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F010120", "EC:6.1.1.16", "UniRef:UniRef50_UPI001F010120", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F010120", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F010120" ], "product": "cysteine--tRNA ligase" } }, "id": "KKNAAMEMLJ_563", "locus": "GCA000027325_00537" }, { "type": "cds", "sequence": "contig_1", "start": 302852, "stop": 304048, "strand": "-", "frame": 2, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47496", "UniRef:UniRef90_P47496" ], "nt": "TTGGAAGCGTTTGACAAAAAACGAGAACAGTTAACTTTTAATCTTGATGGTTTAGTTATTAAACTAAACAGCTTGCTTTTTTATCAACAATTAGGTGCTACAAGTAAATCACCACGTTGGGCAATAGCATTTAAATTTAGTCCTAAATTTGTTCAAACTAAATTAACAGCAGTTCTTATAACGATTGGTAGAACTGGTAGAGTGAACTATACTGCTAAATTAGAAAGTGTTAATTTAGATGGAACAAAAGTAACAGCTGCTACTTTACATAACTTTGATTACATTAAAACTAAAGACATTAGGATCAATGACACTGTTGTTATCTATAAAGCTGGGGAAATTATCCCTAAAGTACTAAAGGTAAATCTTGAAAAAAGAAAAAATGACACTATCATAATTCAAGAGCAAAAATATTGTCCTTCATGTAATTCAAAACTAGTCAAAATAGTTGATGAAGTTGATCAGTATTGTACCAATGAAACTTGTAAGGAGCGAAACATCCAGTTAATTAACTATTTTGTTTCTAAAACTGCTATGGACATTAACGGGTTGAATATTAATACTATTACCAAACTTTATGAACACAATTTGGTTAGATCTATAGTTGATCTTTATGATTTAAAAGACAAGAAAAACCAAGTTTTAAAATTAGATCTGAAGATTGGTGATAAACTTTTCAACAAGTTAGTTGATAACATTGAAAATTCAAAACAAAAAGGAATGGCTAGATTACTAACAGGACTTGGTATTAAGCATGTTGGTAATGTATTAGCTAAGAATTTAGCTAATCATTTTAAAAATATCAAAGCATTACAGCATGCTAGCTTAGAGAACTTAATTAGTTTAAATGATGTAGGAATAACAGTAGCTGAATCATTGTATAACTGGTTTCATGACCCTAACCATTTGCAGTTAATTGAACAACTTGAATTAAGACAAGTAAAAACAGATCAATTACCACTGAAAATTAACTTTGAAACTAACAGTATTTATTTTCAAAAACGCTTTCTTATTACCGGTAGCTTTAACATTAGTCGTGACCAAATTAAGGATTTATTATCAGCTAAGTTTGATTGCCAGTTTGCAAGTGAAGTCAAACCAACAGTTGACTTTGTTATTGCAGGAAACAAACCAACTTTAAGAAAAATCAATCACGCCAAAGAACTGAACATTCCTATCATTAATGAAGCAATTTGA", "aa": "MEAFDKKREQLTFNLDGLVIKLNSLLFYQQLGATSKSPRWAIAFKFSPKFVQTKLTAVLITIGRTGRVNYTAKLESVNLDGTKVTAATLHNFDYIKTKDIRINDTVVIYKAGEIIPKVLKVNLEKRKNDTIIIQEQKYCPSCNSKLVKIVDEVDQYCTNETCKERNIQLINYFVSKTAMDINGLNINTITKLYEHNLVRSIVDLYDLKDKKNQVLKLDLKIGDKLFNKLVDNIENSKQKGMARLLTGLGIKHVGNVLAKNLANHFKNIKALQHASLENLISLNDVGITVAESLYNWFHDPNHLQLIEQLELRQVKTDQLPLKINFETNSIYFQKRFLITGSFNISRDQIKDLLSAKFDCQFASEVKPTVDFVIAGNKPTLRKINHAKELNIPIINEAI", "aa_hexdigest": "3810e779aa5b76cdb2d5b6862c0b2d4b", "start_type": "TTG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 302846, "stop": 304348, "observations": { "mutation": [ 302854, 304111 ], "stop": [ 302854, 304111 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47496", "query_cov": 1.0, "subject_cov": 0.6039453717754173, "identity": 0.997, "score": 691.0, "evalue": 1.59e-245, "gene_start": 1, "gene_end": 398, "reference_start": 260, "reference_end": 657, "reference_sequence": "MMDVKLKIQQLVNLIKNYDYHYYVLSEPLIDDFEYDMLYKSLQQLEKDHPDLIQIDSPTQRVGGEAVKGFKKLNHNSPMLSLENAFSTKEIANFIDNINFQTNSKNEFVVEPKIDGVSISLTYKNGVLVHALTRGDGSVGEDVLNNVKTIKSIPLTIPFTKTIEIRGEIFVDKKTFLAINNQLEKPFANARNLAAGTIRNLNSEITAQRKLRALFYYIPNGLEESITTQTMVLEQLKQWKFPVSDTIRVFQNKFQLINYLEAFDKKREQLTFNLDGLVIKLNSLLFYQQLGATSKSPRWAIAFKFSPKFVQTKLTAVLITIGRTGRVNYTAKLESVNLDGTKVTAATLHNFDYIKTKDIRINDTVVIYKAGEIIPKVLKVNLEKRKNDTIIIQEQKYCPSCNSKLVKIVDEVDQYCTNETCKERNIQLINYFVSKTAMDINGLNINTITKLYEHNLVRSIVDLYDLKDKKNQVLKLDLKIGDKLFNKLVDNIENSKQKGMARLLTGLGIKHVGNVLAKNLANHFKNIKALQHASLENLISLNDVGITVAESLYNWFHDPNHLQLIEQLELRQVKTDQLPLKINFETNSIYFQKRFLITGSFNISRDQIKDLLSAKFDCQFASEVKPTVDFVIAGNKPTLRKINHAKELNIPIINEAIWT" }, "paralog": false, "identity": 0.996, "score": 886.0, "evalue": 0.0, "description": "Internal stop codon at 302854, 304111. Nonsense mutation around 302854, 304111.", "psc": { "uniref90_id": "UniRef90_P47496", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_P47496", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47496", "EC:6.5.1.2", "UniRef:UniRef50_P47496", "COG:COG0272", "COG:L", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47496", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47496" ], "product": "DNA ligase" } }, "id": "KKNAAMEMLJ_564", "locus": "GCA000027325_00538" }, { "type": "cds", "sequence": "contig_1", "start": 304109, "stop": 304822, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23" ], "nt": "ATGGATGTGAAATTGAAGATTCAACAGCTGGTTAACTTAATAAAAAACTATGACTATCACTACTATGTTTTAAGCGAACCTTTAATTGATGATTTTGAGTATGATATGTTGTATAAGTCACTCCAACAATTAGAAAAAGATCATCCTGATTTAATCCAAATTGATTCCCCTACCCAAAGGGTGGGAGGAGAAGCTGTGAAGGGTTTTAAAAAGTTAAACCATAACAGTCCAATGCTCTCTTTGGAAAATGCTTTTTCAACTAAAGAAATTGCTAATTTTATTGATAATATTAACTTTCAAACAAACTCAAAAAATGAATTTGTAGTTGAACCTAAAATTGATGGAGTTAGTATCTCTCTAACTTATAAAAATGGTGTTTTAGTTCATGCTTTAACCAGAGGAGATGGAAGTGTTGGGGAAGATGTTTTAAATAATGTTAAAACCATTAAATCTATCCCTTTAACAATCCCTTTCACAAAAACAATTGAGATTAGGGGTGAGATTTTTGTTGATAAAAAAACTTTTTTAGCAATTAACAATCAACTTGAAAAACCATTTGCTAATGCAAGGAATCTAGCAGCAGGTACAATACGTAATTTAAACAGTGAAATCACTGCACAGCGCAAATTAAGGGCATTATTTTATTACATCCCTAATGGTTTGGAAGAGTCAATCACTACTCAAACTATGGTTTTAGAACAGCTTAAGCAGTGA", "aa": "MDVKLKIQQLVNLIKNYDYHYYVLSEPLIDDFEYDMLYKSLQQLEKDHPDLIQIDSPTQRVGGEAVKGFKKLNHNSPMLSLENAFSTKEIANFIDNINFQTNSKNEFVVEPKIDGVSISLTYKNGVLVHALTRGDGSVGEDVLNNVKTIKSIPLTIPFTKTIEIRGEIFVDKKTFLAINNQLEKPFANARNLAAGTIRNLNSEITAQRKLRALFYYIPNGLEESITTQTMVLEQLKQ", "aa_hexdigest": "c38251d25be9a4c74c59183158d0b399", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 181, "aa_cov": 0.7172995780590717, "hmm_cov": 1.0, "evalue": 1.743038405700815e-65, "score": 216.0682830810547, "start": 68, "stop": 237 } ], "seq_stats": { "molecular_weight": 26954.529200000026, "isoelectric_point": 6.122093772888182 }, "id": "KKNAAMEMLJ_565", "locus": "GCA000027325_00539" }, { "type": "tRNA", "sequence": "contig_1", "start": 304892, "stop": 304965, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 80.2, "nt": "GCAGATATAGTTCAATGGTAGAACATAACCTTGCCAAGGTTAAGATGCGGGTTCGATTCCCGTTATCTGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 304931, 304933 ], "id": "KKNAAMEMLJ_32", "locus": "GCA000027325_00540" }, { "type": "cds", "sequence": "contig_1", "start": 305001, "stop": 305294, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCTGAAAACCAAATTGCAATCCTGGATTATATTTTTAACCAAGTCAACCAGCCCAATCAACCAAAAATAGTTTGGTTCTCTGGGGAAGGGGAGGATGAGAAAATTAATTTTTTAATCCGCTTAAATGATTTTTTTAAACCAAAATTTGTTGAAAATACTAATGATAGTAGCTTTTTATTAAGTTTTAGAAACCATGTTGAAACTAAAAATTCAACGCCTTTAACCCAAGCTAACTTTGCTAATATTGCCAACAAATTACTAGCGGTTTTATTTGGTTCATTGCAATGA", "aa": "MESENQIAILDYIFNQVNQPNQPKIVWFSGEGEDEKINFLIRLNDFFKPKFVENTNDSSFLLSFRNHVETKNSTPLTQANFANIANKLLAVLFGSLQ", "aa_hexdigest": "8442183bc8b656aa3f9e060de47a4d1b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11153.428599999983, "isoelectric_point": 4.967807579040527 }, "id": "KKNAAMEMLJ_566", "locus": "GCA000027325_00541" }, { "type": "cds", "sequence": "contig_1", "start": 305424, "stop": 306098, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG255", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47497", "UniRef:UniRef90_P47497" ], "nt": "TTGAGTTTTATTAAACAACAATGGCCTATTCTAACAACATTGGTAACAGTAGGGACCACATTAGGTACTCCAGTATTTTCATTAACAATAGCCCAACAAGATGGTATTAAGCAAAATGCGGGGAATGATGTCTTTATCTTTTTGATTATCTTCTCTGTTTTTTCTATTAGTTTAGGGCTTGTTTCATCACTTATCTTTTTAGTCTCCAGCTTATTTTCAATTCGTCAGAAAAAAACGTTGGATGCTTTGGATAAAGTGTTATCAAAATTTATTGATAAATACTTTTTTTTAGATGAAAAAGAGATCAAAAAACAACTTAAATATCAGTTTAAAAACAACGGTGTTTGCTTTTTTTATGGTTTTGATTTTGATCAGGCAGAGTTTCTTGAACAATCAATGAACTTAATGTTGTTGTTAAAGCAAACCAATTGTTTTATTTTAGTTGGTTGTAAAGAAAGTGAAATGACGCTCATTAAAAACAAGATAGAACCTAACATTAACTTAAAACAAAACAGTTTTTATCTGGATTTAAGCAATGAAATTTCACAAGTAGAACAGATCAGCAAATTTAACTTGTTGTTTAGGCAATTGAGGCTCAGTTCTGAACTATTTTACTTAGAAGATTTTTTTGATTATCTAACTACTGCAAAACAGATAGTTAACTTTCTTTTTTAA", "aa": "MSFIKQQWPILTTLVTVGTTLGTPVFSLTIAQQDGIKQNAGNDVFIFLIIFSVFSISLGLVSSLIFLVSSLFSIRQKKTLDALDKVLSKFIDKYFFLDEKEIKKQLKYQFKNNGVCFFYGFDFDQAEFLEQSMNLMLLLKQTNCFILVGCKESEMTLIKNKIEPNINLKQNSFYLDLSNEISQVEQISKFNLLFRQLRLSSELFYLEDFFDYLTTAKQIVNFLF", "aa_hexdigest": "abef48617e61c0bd0d81bff68dcf3226", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 305124, "stop": 306098, "observations": { "mutation": [ 305292, 305322, 305370 ], "stop": [ 305292, 305322, 305370 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47497", "query_cov": 1.0, "subject_cov": 0.6136986301369863, "identity": 0.996, "score": 420.0, "evalue": 5.5e-146, "gene_start": 1, "gene_end": 224, "reference_start": 142, "reference_end": 365, "reference_sequence": "MESENQIAILDYIFNQVNQPNQPKIVWFSGEGEDEKINFLIRLNDFFKPKFVENTNDSSFLLSFRNHVETKNSTPLTQANFANIANKLLAVLFGSLQWKQLNKPTGNWFLVILFLALLWLRQCWLKLQLTKISKFVNQKGILSFIKQQWPILTTLVTVGTTLGTPVFSLTIAQQDGIKQNAGNDVFIFLIIFSVFSISLGLVSSLIFLVSSLFSIRQKKTLDALDKVLSKFIDKYFFLDEKEIKKQLKYQFKNNGVCFFYGFDFDQAEFLEQSMNLMLLLKQTNCFILVGCKESEMTLIKNKIEPNINLKQNSFYLDLSNEISQVEQISKFNLLFRQLRLSSELFYLEDFFDYLTTAKQIVNFLF" }, "paralog": false, "identity": 0.9907407407407407, "score": 622.0, "evalue": 2.34e-228, "description": "Internal stop codon at 305292, 305322, 305370. Nonsense mutation around 305292, 305322, 305370.", "psc": { "uniref90_id": "UniRef90_P47497", "product": "Uncharacterized protein MG255", "uniref50_id": "UniRef50_P47497", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47497", "UniRef:UniRef50_P47497", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47497", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47497" ], "product": "Uncharacterized protein MG255" } }, "id": "KKNAAMEMLJ_567", "locus": "GCA000027325_00542" }, { "type": "tRNA", "sequence": "contig_1", "start": 306617, "stop": 306691, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 54.4, "nt": "CTTCCCTTGGTGCAATGGACAGCACAACTGAGTCCTAATCAGTAAATAGAGGTTCAACTCCTCTAGGGAAGGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 306621, 306623 ], "id": "KKNAAMEMLJ_10", "locus": "GCA000027325_00543" }, { "type": "tRNA", "sequence": "contig_1", "start": 306740, "stop": 306813, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 75.5, "nt": "GGGGGTGTAGTTTAGTGGTAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 306772, 306774 ], "id": "KKNAAMEMLJ_11", "locus": "GCA000027325_00544" }, { "type": "cds", "sequence": "contig_1", "start": 306821, "stop": 307000, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTCAATTCAAACTTTAAAGAATGCTTTAATAAGATTGCAAAAAAAGTTAATTCACTGGACAGTGAATACTATGAGTTTTCTTCTTTTATTGAAAGGATTAGAACTACCTTTGGACTTTTAATTGCTTTAACTGTTTTAAGTAACCTAATCATTATTAGCTTTGTTTTAATTTGA", "aa": "MHFNSNFKECFNKIAKKVNSLDSEYYEFSSFIERIRTTFGLLIALTVLSNLIIISFVLI", "aa_hexdigest": "9614e6544800b86cf8eb54ecc2a5c817", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6879.990399999995, "isoelectric_point": 7.90815944671631 }, "id": "KKNAAMEMLJ_568", "locus": "GCA000027325_00545" }, { "type": "cds", "sequence": "contig_1", "start": 307202, "stop": 307342, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGATTAAATAAAACTATTAAGAATTTCGCTAGTGATTATGGTTTTAAGATTGTTAATAAATATAACAGTTTAACAAGCGGAATTTTTGTGTTTGACTTTGCTGATTATGTTAGTTTTGAAGCCAACCTGACTAATTGA", "aa": "MRLNKTIKNFASDYGFKIVNKYNSLTSGIFVFDFADYVSFEANLTN", "aa_hexdigest": "9349d36f0229c30105ecd7763246f589", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5304.936200000001, "isoelectric_point": 8.063141441345213 }, "id": "KKNAAMEMLJ_569", "locus": "GCA000027325_00546" }, { "type": "cds", "sequence": "contig_1", "start": 307637, "stop": 307930, "strand": "+", "frame": 2, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010869398.1", "SO:0001217", "UniParc:UPI0000133F16", "UniRef:UniRef100_P47499", "UniRef:UniRef50_P47499", "UniRef:UniRef90_P47499" ], "nt": "ATGAAAAAGGCAATCCACTTTCAGAGTCAACCAGTTGTTTTTAACTGTGCTTCATGCAATAGCAACTTTACCATTGACTCCACTGCCAAACAAAAGGATCTTGCCATTGACATTTGTGGAAAATGTCATCCTTTTTACATAGGGCAATTAACCAAACAAACCGTGCATGGACGGGCTGAAAAACTTTCTCAAAAGTTCAACGCTGGAAAGGCTTTTTTAGAAAATAAAACTAAAAAGAGTAACCAAGCTAAAGTTGAAAAACAAACTAGGCACCGTTCTATTAACGAGCTTTAG", "aa": "MKKAIHFQSQPVVFNCASCNSNFTIDSTAKQKDLAIDICGKCHPFYIGQLTKQTVHGRAEKLSQKFNAGKAFLENKTKKSNQAKVEKQTRHRSINEL", "aa_hexdigest": "6c80e470856c3c995d51b22f650d2d60", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133F16", "ncbi_nrp_id": "WP_010869398.1", "uniref100_id": "UniRef100_P47499", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133F16", "RefSeq:WP_010869398.1", "UniRef:UniRef100_P47499" ] }, "ips": { "uniref100_id": "UniRef100_P47499", "uniref90_id": "UniRef90_P47499" }, "psc": { "uniref90_id": "UniRef90_P47499", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_P47499", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47499" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "KKNAAMEMLJ_570", "locus": "GCA000027325_00547" }, { "type": "cds", "sequence": "contig_1", "start": 307930, "stop": 308370, "strand": "+", "frame": 1, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_P47500", "UniRef:UniRef90_P47500" ], "nt": "GTGGATTTTGACAAACAACTCTTTTTCAATGTTGAAAAGATTGTTGAACTTACTGAACAACTTGAAAAAGATCTCAATAAACCTAACCTTAGCTTTGAGCAGATTAAAGTTATTAACAAAGAGTTAAAACATAAACAACCTTTAATAGTTAAATTCAAGGAGTTGCAAAAGCTGGTTGAAAATGCTAATGAAGCTGAACAAATTCTTAACAATTCCAGCTTAAAAGAATTGCATGAAGAAGCTAAAAAAGAACTTGAAAAAATCAAAGCTAGTTTACCTAGTTTAGAAGAGGAAATTAAGTTCCTTTTACTACCGGTAGATGAAAATAACCAAAAGAATGTTATTGTAGAGATCCGTCCAGCTGCTGGTGGGGATGAATCTTGCATCTTCTTAAGTGATCTTTTTAATATGTACAAAAACTATTGTACTAGTAAAAATTGA", "aa": "MDFDKQLFFNVEKIVELTEQLEKDLNKPNLSFEQIKVINKELKHKQPLIVKFKELQKLVENANEAEQILNNSSLKELHEEAKKELEKIKASLPSLEEEIKFLLLPVDENNQKNVIVEIRPAAGGDESCIFLSDLFNMYKNYCTSKN", "aa_hexdigest": "79b475bf50048d446eca0dccdb6baad5", "start_type": "GTG", "rbs_motif": null, "genes": [ "prfA" ], "truncated": "3-prime", "pseudogene": { "start": 307930, "stop": 308673, "observations": { "mutation": [ 308368 ], "stop": [ 308368 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47500", "query_cov": 1.0, "subject_cov": 0.40668523676880225, "identity": 1.0, "score": 273.0, "evalue": 1.4e-89, "gene_start": 1, "gene_end": 146, "reference_start": 1, "reference_end": 146, "reference_sequence": "MDFDKQLFFNVEKIVELTEQLEKDLNKPNLSFEQIKVINKELKHKQPLIVKFKELQKLVENANEAEQILNNSSLKELHEEAKKELEKIKASLPSLEEEIKFLLLPVDENNQKNVIVEIRPAAGGDESCIFLSDLFNMYKNYCTSKNWTVELNEIIPASVGINFVSFAVNGTDVFAKLKFESGVHRVQRVPLTEAKGRVHTSTVTVAVLPQLEEVEITINPSDLRIDTYRASGAGGQHVNRTESAVRITHLPTGIVVACQEGKSQFSNRDKAMKMLRAKLWENAQNKQLSTQADLRKSQVGSGERAEKIRTYNYPQNRITDHRIKLTINKLNTVILGDLDEIIEALQADEKKQQLEKFIS" }, "paralog": false, "identity": 0.9919028340080972, "score": 475.0, "evalue": 1.22e-171, "description": "Internal stop codon at 308368. Nonsense mutation around 308368.", "psc": { "uniref90_id": "UniRef90_P47500", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_P47500", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47500", "UniRef:UniRef50_P47500", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_P47500", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47500" ], "product": "Peptide chain release factor 1" } }, "id": "KKNAAMEMLJ_571", "locus": "GCA000027325_00548" }, { "type": "cds", "sequence": "contig_1", "start": 308404, "stop": 309009, "strand": "+", "frame": 1, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_P47500", "UniRef:UniRef90_P47500" ], "nt": "GTGGGGATTAACTTTGTTTCTTTTGCGGTAAATGGTACTGATGTTTTTGCTAAACTCAAGTTTGAATCAGGAGTACATAGGGTGCAACGTGTTCCTTTAACAGAAGCTAAAGGTAGAGTGCATACCTCAACAGTTACTGTTGCTGTTTTACCTCAATTAGAAGAGGTAGAGATCACCATTAATCCTAGTGATTTGAGAATCGATACTTACCGTGCTTCAGGAGCGGGTGGACAACATGTTAACAGAACTGAAAGTGCAGTTAGAATTACCCATCTACCTACGGGAATTGTTGTGGCTTGCCAAGAGGGTAAATCCCAGTTTTCTAACCGTGATAAAGCAATGAAAATGCTACGTGCTAAGTTATGGGAAAATGCTCAAAATAAACAACTCTCAACCCAAGCGGATTTAAGAAAAAGTCAAGTTGGTAGTGGAGAGAGAGCTGAAAAAATTCGTACCTACAACTATCCTCAAAACAGAATTACAGACCACAGGATTAAATTAACTATTAATAAGCTTAATACTGTTATTTTGGGAGATCTTGATGAGATCATTGAAGCTTTACAAGCTGATGAAAAGAAACAACAGTTGGAAAAGTTTATTAGTTAG", "aa": "MGINFVSFAVNGTDVFAKLKFESGVHRVQRVPLTEAKGRVHTSTVTVAVLPQLEEVEITINPSDLRIDTYRASGAGGQHVNRTESAVRITHLPTGIVVACQEGKSQFSNRDKAMKMLRAKLWENAQNKQLSTQADLRKSQVGSGERAEKIRTYNYPQNRITDHRIKLTINKLNTVILGDLDEIIEALQADEKKQQLEKFIS", "aa_hexdigest": "c2cc6985177949825b26e2cd5375f320", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_K1ZX70", "query_cov": 0.9552238805970149, "subject_cov": 0.8421052631578947, "identity": 0.62, "score": 246.0, "evalue": 7.87e-80, "valid": false, "product": "Peptide chain release factor 1", "uniref50_id": "UniRef50_A0A2C9JR53", "kegg_orthology_id": "K02835" }, "genes": [ "prfA" ], "truncated": "5-prime", "pseudogene": { "start": 308104, "stop": 309009, "observations": { "mutation": [ 308368 ], "stop": [ 308368 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47500", "query_cov": 1.0, "subject_cov": 0.5598885793871866, "identity": 0.995, "score": 386.0, "evalue": 3e-133, "gene_start": 1, "gene_end": 201, "reference_start": 159, "reference_end": 359, "reference_sequence": "MDFDKQLFFNVEKIVELTEQLEKDLNKPNLSFEQIKVINKELKHKQPLIVKFKELQKLVENANEAEQILNNSSLKELHEEAKKELEKIKASLPSLEEEIKFLLLPVDENNQKNVIVEIRPAAGGDESCIFLSDLFNMYKNYCTSKNWTVELNEIIPASVGINFVSFAVNGTDVFAKLKFESGVHRVQRVPLTEAKGRVHTSTVTVAVLPQLEEVEITINPSDLRIDTYRASGAGGQHVNRTESAVRITHLPTGIVVACQEGKSQFSNRDKAMKMLRAKLWENAQNKQLSTQADLRKSQVGSGERAEKIRTYNYPQNRITDHRIKLTINKLNTVILGDLDEIIEALQADEKKQQLEKFIS" }, "paralog": false, "identity": 0.9966777408637874, "score": 583.0, "evalue": 3.66e-213, "description": "Internal stop codon at 308368. Nonsense mutation around 308368.", "psc": { "uniref90_id": "UniRef90_P47500", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_P47500", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47500", "UniRef:UniRef50_P47500", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_P47500", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47500" ], "product": "Peptide chain release factor 1" } }, "id": "KKNAAMEMLJ_572", "locus": "GCA000027325_00549" }, { "type": "cds", "sequence": "contig_1", "start": 309010, "stop": 309135, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCTGTATGAGTTTTTTTTAAATCAAAAGTTAGTTTACCAATCCAGTCCCCATTTTAACGGGGTATTTTTAACAATATTGGAACACTATGGTTTTCAATTTAAAACAATTGATAAACTCTGA", "aa": "MTLYEFFLNQKLVYQSSPHFNGVFLTILEHYGFQFKTIDKL", "aa_hexdigest": "dbaef87e3742785d0b97ab90629f305c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4958.684300000001, "isoelectric_point": 6.697759056091309 }, "id": "KKNAAMEMLJ_573", "locus": "GCA000027325_00550" }, { "type": "cds", "sequence": "contig_1", "start": 309217, "stop": 309492, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13649.12", "PFAM:PF05175.20" ], "nt": "TTGCCCTATTTGTTGGGAACAATTCAACTAAGGAAGCTTACTTTTAAAACTAAGAAAGGAGTTTTTATTCCTCGAATTGATAGCTTAGCACTAATTGCAAGTGTTAACTTAAAAAAAATAAAAACTGCACTTGACCTTTGTTGTGGTTCAGGTACTTTAGCCATTGCTTTAAAAAAGAAGTGTGATACACTTGATGTTTATGGTAGTGATATTGATATCCAAGCATTAAAACTAGCGCAACAAAATGCATTAATTAATAACGTTAGTATTAATTGA", "aa": "MPYLLGTIQLRKLTFKTKKGVFIPRIDSLALIASVNLKKIKTALDLCCGSGTLAIALKKKCDTLDVYGSDIDIQALKLAQQNALINNVSIN", "aa_hexdigest": "74af16969ad8a5c5c05b3ea180c1a804", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13649.12", "name": "Methyltransf_25", "length": 41, "aa_cov": 0.45054945054945056, "hmm_cov": 1.0, "evalue": 2.5325147551339348e-08, "score": 29.63446044921875, "start": 44, "stop": 84 }, { "id": "PF05175.20", "name": "MTS", "length": 80, "aa_cov": 0.8461538461538461, "hmm_cov": 1.0, "evalue": 5.78074342259994e-13, "score": 43.91481399536133, "start": 13, "stop": 89 } ], "seq_stats": { "molecular_weight": 9890.738899999991, "isoelectric_point": 9.585433387756346 }, "id": "KKNAAMEMLJ_574", "locus": "GCA000027325_00551" }, { "type": "cds", "sequence": "contig_1", "start": 309619, "stop": 310050, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTTTCAAAATAAAAATAGTTATTTTGCATACAAGCAGTTGTTTAATCTATTACTAACAAAACGATCAATTAAACAGTTAATTTTTGAATGTTCTTTATTTCAAAAAGAAAGGCTATTAAATTTGTTTTCAATCTTTAAATCAAGGCCGATTTTTAACTTTCAAAAACAGTTTATTGGTATGAAAGTTGATAATCAAAAACTCCCAGTAGTTGATATTAAAAATACCAAAACTATTAAGCAACTTTTAAAAATGGGGCTAGCAGGAATTGTAAATACTGATACACAAATGGGATTAATTAGTTATTCAGAGTCTACTCTTGACAAAATTAAACAACGTGCACTTAACAAACATTATGTATCAATGTTTGGGTTAGAAGAATTAAAGAAGTTACCAAAAAAACTACAACAAATTGCTAGTTACTTTTGA", "aa": "MVFQNKNSYFAYKQLFNLLLTKRSIKQLIFECSLFQKERLLNLFSIFKSRPIFNFQKQFIGMKVDNQKLPVVDIKNTKTIKQLLKMGLAGIVNTDTQMGLISYSESTLDKIKQRALNKHYVSMFGLEELKKLPKKLQQIASYF", "aa_hexdigest": "0149733c06491adca5ee57a3bf28c3e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16795.792999999983, "isoelectric_point": 10.03767910003662 }, "id": "KKNAAMEMLJ_575", "locus": "GCA000027325_00552" }, { "type": "cds", "sequence": "contig_1", "start": 310511, "stop": 311146, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCTCAAAGGTTTTTTAGCTGTTGGTGTTAGTGTTTTTGGTTTTTCTGGTTTACTGATGGCTTGTAGTGTTGTAAGTCAGTTTGATCAAGTGGATGATGGCAAAATTAAGCTAGCATCTTCATTAACTTCGAAACGCGCAGCGGAAGCTTTAGAGACAGTAGTTAAAAAATATAACGATACTAAAGATCCTGGTGATTATCCAATTGAAATAGTACAAATTGCTGGGGGTTATGATGGGGGTAAAAAGGATGTTCAAACCAAGGTTAGTACCAAAGATAAAAATAACTTTTATAACCTGATTTTAAACTATCCAGAAATAGTAAGTACCTTATCAAGGTCTAAAATGGCTTTGAATTTTGATGGGGTTAATGTTGATAAACTCCATCCTAACTTTTTAAGCTTTAACAGCAGAATAGGTGGAATTAGAGATGATGGAATCTATGCTATTCCAATATCTATGTCCACTGATCTGATGGTCATTAATGGCCCTGTTTTACACTATATTCTAAACAGTGCTAGAAAAGAAGGTACACCAACTAGCACTACTGTTCAAGCAACTGTCAGTTCAAGAAGTGCAGAAAAAAAAGGTACATTAGAAATTGCAAATGATAGTGAAACTACTAAACTTTGA", "aa": "MKLKGFLAVGVSVFGFSGLLMACSVVSQFDQVDDGKIKLASSLTSKRAAEALETVVKKYNDTKDPGDYPIEIVQIAGGYDGGKKDVQTKVSTKDKNNFYNLILNYPEIVSTLSRSKMALNFDGVNVDKLHPNFLSFNSRIGGIRDDGIYAIPISMSTDLMVINGPVLHYILNSARKEGTPTSTTVQATVSSRSAEKKGTLEIANDSETTKL", "aa_hexdigest": "dfe8ca7c1eda06638be0967a630571ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22836.78190000001, "isoelectric_point": 8.499463462829592 }, "id": "KKNAAMEMLJ_576", "locus": "GCA000027325_00553" }, { "type": "cds", "sequence": "contig_1", "start": 311663, "stop": 312808, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P47502" ], "nt": "TTGTTTGTTAACCGTGGTGGTTCCTATTCATCCAACTTTGAAAAATTTCACCAATTGGCATTCTCAGTATCTTCTTCTGGAGGATACAGTTATTACTTTGCTAAAGATAATGCTAAGCGCTTAAAGTTTAGTAATTATGCTATTGAATATCCTAGTTTTACCCAAACAATTCAAGCTCCTAATTCTTCAGAAACAGAAAGTAATTTACTTGGTACTTTTAAATTAAGTGAAAAAGATATCAATCTATATAAAGGTTCAATTCCTAGTGGAAAACAACAAGGAGTTGATGCTATCTTAATTAGTAACCCAAACTTAATTAATATTCTTGAACAAGCAAAACAAAAAAACACTGCACAAGGAAGTGAATCAACCACTAACAAGATAATAGGTTATACCACCACTGCAAATGTTAATGTTGATAATCAAAACATCTTTTCTGTTAGCAAACTTAACAACGAACAGTTTCAAAGAAAAATCATTGTTAATGCCACTGAAGAAACACTTGATCAATCCCAAACCTTACAGAGCAATGAATCAATTGTTTTACCAATGCCTGGTAAATACAAATCAACTGATAAAAATAAAGTAATGATCACCCAAGGTCCTAACTTAATAGGCATCCATGCAAATGAAAAAGAAAATATTGAAACTAAAAAATTTGTTAATTGGTTTTTAAATCAAAGTATTACAGATTGGAATAGTAATAATCAGCAAAAAAATAGTGATCAAACAACAAAAACTGCTGCTGAATATTTCACTGATCAAGCTTCTTACATCCTTCCTTTAAAGGAAAAATTTAACAAAAGTTCAGATTTAGAATTGAAAGGCAGTAGTAGTTCTTCTAATTTAACAACCAGTAGTGCTAGCGCCTCTTTGTTAATAAGTAATAATAGTTCAACTGCAAGTTCTCCTGCTCCTAAAAAAACAAACAATAATTCTAATACCTTTACAGCTAAAGCACTAGAATTATTCCAACAAGCTGCTAACAATGAAATTATTCCCTTTAGTGATCCAAGTGACTTTAGGAACGGCACATTCCGTAATAATATAAGCAGTAGTTTTAATGCTGCGGTGAATTCTAAGGTTAGTTTTAATCAATTTGTCCAAAACTTTATTAATAGTTTAGGATCTGGATTTAGAAGATAA", "aa": "MFVNRGGSYSSNFEKFHQLAFSVSSSGGYSYYFAKDNAKRLKFSNYAIEYPSFTQTIQAPNSSETESNLLGTFKLSEKDINLYKGSIPSGKQQGVDAILISNPNLINILEQAKQKNTAQGSESTTNKIIGYTTTANVNVDNQNIFSVSKLNNEQFQRKIIVNATEETLDQSQTLQSNESIVLPMPGKYKSTDKNKVMITQGPNLIGIHANEKENIETKKFVNWFLNQSITDWNSNNQQKNSDQTTKTAAEYFTDQASYILPLKEKFNKSSDLELKGSSSSSNLTTSSASASLLISNNSSTASSPAPKKTNNNSNTFTAKALELFQQAANNEIIPFSDPSDFRNGTFRNNISSSFNAAVNSKVSFNQFVQNFINSLGSGFRR", "aa_hexdigest": "6b187ebffdb5d07938715c5c646df684", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 311363, "stop": 312808, "observations": { "mutation": [ 311366 ], "stop": [ 311366 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47502", "query_cov": 1.0, "subject_cov": 0.4980392156862745, "identity": 0.997, "score": 647.0, "evalue": 8.33e-227, "gene_start": 1, "gene_end": 381, "reference_start": 385, "reference_end": 765, "reference_sequence": "MKLKGFLAVGVSVFGFSGLLMACSVVSQFDQVDDGKIKLASSLTSKRAAEALETVVKKYNDTKDPGDYPIEIVQIAGGYDGGKKDVQTKVSTKDKNNFYNLILNYPEIVSTLSRSKMALNFDGVNVDKLHPNFLSFNSRIGGIRDDGIYAIPISMSTDLMVINGPVLHYILNSARKEGTPTSTTVQATVSSRSAEKKGTLEIANDSETTKLWQNIQTTAQNNSNETTKEQKQVKRSSSSSSTTSTTGETKDTTKSDNKIKEFWGEYQEVDGGLKNFTFKASIFENWNETLDFATRIANSFPEKVKNITNKTGLDLQGVLGVDSSSNALYAAVFAAGQANYDNFFFNIDKRTGYADYSNFLNKDSSYQNLESVYNDFYKLIQANGLFVNRGGSYSSNFEKFHQLAFSVSSSGGYSYYFAKDNAKRLKFSNYAIEYPSFTQTIQAPNSSETESNLLGTFKLSEKDINLYKGSIPSGKQQGVDAILISNPNLINILEQAKQKNTAQGSESTTNKIIGYTTTANVNVDNQNIFSVSKLNNEQFQRKIIVNATEETLDQSQTLQSNESIVLPMPGKYKSTDKNKVMITQGPNLIGIHANEKENIETKKFVNWFLNQSITDWNSNNQQKNSDQTTKTAAEYFTDQASYILPLKEKFNKSSDLELKGSSSSSNLTTSSASASLLISNNSSTASSPAPKKTNNNSNTFTAKALELFQQAANNEIIPFSDPSDFRNGTFRNNISSSFNAAVNSKVSFNQFVQNFINSLGSGFRR" }, "paralog": false, "identity": 0.997920997920998, "score": 867.0, "evalue": 4.15e-316, "description": "Internal stop codon at 311366. Nonsense mutation around 311366.", "psc": { "uniref90_id": "UniRef90_P47502", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47502", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "KKNAAMEMLJ_577", "locus": "GCA000027325_00554" }, { "type": "cds", "sequence": "contig_1", "start": 312846, "stop": 313193, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTAAGGTGGCATTAGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCTTTTGATCCCACCCAAGGGTTGCAAAAAGATAGTCCAGTGAAGGATTCAAGTAAACAAGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTATGGCTACCTCTACAAGAGATAAAGCCCTCAAGATTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAGAACAGTAGTGGGACAGAGGTGAAGTTGGATTCACAGAAGGATTTTCCCCAAGGAAAGGTTTGA", "aa": "MPKVALEYHNLNNQVVKESLEVEATDSFDPTQGLQKDSPVKDSSKQGEKLQETMSSMSGMATSTRDKALKIEVERGSQSDSLLKNDFAKKPLKHKNSSGTEVKLDSQKDFPQGKV", "aa_hexdigest": "4fa8ebd230e0708f120f22a9cd0c58d7", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00030561CF", "query_cov": 0.9565217391304348, "subject_cov": 0.8396946564885496, "identity": 0.8270000000000001, "score": 162.0, "evalue": 1.18e-49, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12712.097699999988, "isoelectric_point": 6.7376600265502935 }, "id": "KKNAAMEMLJ_578", "locus": "GCA000027325_00555" }, { "type": "cds", "sequence": "contig_1", "start": 313108, "stop": 313374, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCTACGCCAATGTTAGAAAAAGTGGGGAGTGGGGTTGAGGAAGCGGAAGTTGAAGGGGAGGGGGAGTTGGAGAAGGGTTTACCATTGCCGATTCAGGGGAGAAAGTCTAAGTCGCCCCCATCCCCCTATTTTTTTCTATCTCATCTGTTTTCAACACCGGTTTTCAAACCTTTCCTTGGGGAAAATCCTTCTGTGAATCCAACTTCACCTCTGTCCCACTACTGTTCTTATGTTTCAGTGGCTTTTTAG", "aa": "MIIDLTPTPMLEKVGSGVEEAEVEGEGELEKGLPLPIQGRKSKSPPSPYFFLSHLFSTPVFKPFLGENPSVNPTSPLSHYCSYVSVAF", "aa_hexdigest": "ca90c77ed984269fd7731720239e81f7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9581.857599999996, "isoelectric_point": 4.980084800720214 }, "id": "KKNAAMEMLJ_579", "locus": "GCA000027325_00556" }, { "type": "cds", "sequence": "contig_1", "start": 313496, "stop": 313768, "strand": "+", "frame": 2, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDQ4", "UniRef:UniRef90_UPI00030FFC1F" ], "nt": "GTGAAACCTAGTAGTAGTAGCAACACGTTTAATCCCAATTCAGATGATAATAAAGTCACACCATCAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACCAATAAGAATAACCCCCAGCGCAATCAGTTGTTGTTAAGAGCGTTATTAGGAACTATTCCGGTCTTGATCAATAAGAGTGGAACGGGAGATCAATTTAACAAGGATAGTGAGCAAAAATGA", "aa": "MKPSSSSNTFNPNSDDNKVTPSGGSSKPTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGTGDQFNKDSEQK", "aa_hexdigest": "51b3e39d83a2b7ed0f2e4d0702238467", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 313367, "stop": 313879, "observations": { "mutation": [ 313436, 313766 ], "stop": [ 313436, 313766 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00030FFC1F", "query_cov": 1.0, "subject_cov": 0.5294117647058824, "identity": 0.9890000000000001, "score": 176.0, "evalue": 9.72e-55, "gene_start": 1, "gene_end": 90, "reference_start": 44, "reference_end": 133, "reference_sequence": "MITQHLNQQNTRWVFTPGSTPDIWTGAGYRKANNNNNGIPFEQVKPSSSSNTFNPNSDDNKVTPSGGSSKPTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGTGDQFNKDSEQKWNETDKLGGNLPGFGEVNGASYKIFTYLIIKKSVRFF" }, "paralog": false, "identity": 0.9882352941176471, "score": 345.0, "evalue": 4.57e-124, "description": "Internal stop codon at 313436, 313766. Nonsense mutation around 313436, 313766.", "psc": { "uniref90_id": "UniRef90_UPI00030FFC1F", "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00030FFC1F", "UniRef:UniRef50_A0A7M3VDQ4" ] } }, "id": "KKNAAMEMLJ_580", "locus": "GCA000027325_00557" }, { "type": "cds", "sequence": "contig_1", "start": 314354, "stop": 314527, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGAAAAAGTATCAGTTAGATGTACCGAATGTTACCAATGAGATGAACGATGTACTGAAAGAATTGATCCTAGAACAACCCCTTACCAAGTATACCTTAAACAGTAGTTTGGCCAAAGAAAAGGGCAAAACCCAAAGGGAGGTGCATCTGGGTAGTGGGCAAGCAACTAATTGA", "aa": "MKKYQLDVPNVTNEMNDVLKELILEQPLTKYTLNSSLAKEKGKTQREVHLGSGQATN", "aa_hexdigest": "03a08e38588e8403f5b59586aa8ad10f", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 314225, "stop": 314586, "observations": { "deletion": [ 314543 ], "mutation": [ 314345, 314525 ], "stop": [ 314345, 314525, 314558 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 0.9824561403508771, "subject_cov": 0.4628099173553719, "identity": 0.804, "score": 91.3, "evalue": 1.64e-22, "gene_start": 1, "gene_end": 56, "reference_start": 43, "reference_end": 98, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7272727272727273, "score": 163.0, "evalue": 1.24e-53, "description": "Internal stop codon at 314345, 314525, 314558. Frameshift due to deletion around 314543. Nonsense mutation around 314345, 314525.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6445.293300000001, "isoelectric_point": 8.03052043914795 }, "id": "KKNAAMEMLJ_581", "locus": "GCA000027325_00558" }, { "type": "cds", "sequence": "contig_1", "start": 314714, "stop": 315232, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49246", "UniRef:UniRef90_UPI0005A23939" ], "nt": "ATGGCAGCAGGGGATGCGCCCCTAAGTACAGGAGGGAGATCATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGAAAGGATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTACCCAACTCTACCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAGGTTAATTGAGCCTGACAATGCAACAAGTGCAGCGAGCAGCATGACCAGCTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAACAGAAGTTGGGGAAGGGGGGAACAGCAATGCAGGGAAATAATGGTGGAGGGGGTGTTAGTCAAACGATTAACACCATTACCACTACGGGAAATATTAGTGGCAATGGAACCATTCAAACGGCTTATCCGGTGAAAAAAGATGAAGCTTCAAATGTAGCGATCAATTCCTTGATTAACGCTACGCCCTTGAATAGTTATGGGGATTTAAATAATGCTAGTTTTTCTAAATAA", "aa": "MAAGDAPLSTGGRSSDQSNKFTKYLNTKQALERIGILFDGDGMRNVVTQLYQPNKVKSGQYQQNNTYNRLIEPDNATSAASSMTSLLKLLSSKNIKQKLGKGGTAMQGNNGGGGVSQTINTITTTGNISGNGTIQTAYPVKKDEASNVAINSLINATPLNSYGDLNNASFSK", "aa_hexdigest": "1320c702c5291ee583dbf81d98e6630f", "start_type": "ATG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "5-prime", "pseudogene": { "start": 314414, "stop": 315232, "observations": { "mutation": [ 314525, 314633 ], "stop": [ 314525, 314633 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005A23939", "query_cov": 1.0, "subject_cov": 0.5134328358208955, "identity": 1.0, "score": 319.0, "evalue": 1.16e-107, "gene_start": 1, "gene_end": 172, "reference_start": 164, "reference_end": 335, "reference_sequence": "MKLTYSSSGSNNQISFDSTSQGEKPSYVVEFTNSTNIGIKWRVVKKYQLDVPNVTNEMNDVLKELILEQPLTKYTLNSSLAKEKGKTQREVHLGSGQATNWRSMRNSIGLNDNPSPNASTGFKLDKGNAYRKLSESWPIYQPIDGTKQGKGKDSNGWSSTEATMAAGDAPLSTGGRSSDQSNKFTKYLNTKQALERIGILFDGDGMRNVVTQLYQPNKVKSGQYQQNNTYNRLIEPDNATSAASSMTSLLKLLSSKNIKQKLGKGGTAMQGNNGGGGVSQTINTITTTGNISGNGTIQTAYPVKKDEASNVAINSLINATPLNSYGDLNNASFSK" }, "paralog": false, "identity": 0.9926470588235294, "score": 531.0, "evalue": 8.65e-194, "description": "Internal stop codon at 314525, 314633. Nonsense mutation around 314525, 314633.", "psc": { "uniref90_id": "UniRef90_UPI0005A23939", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_Q49246", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005A23939", "UniRef:UniRef50_Q49246" ] }, "pscc": { "uniref50_id": "UniRef50_Q49246", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49246" ], "product": "MgPa" } }, "id": "KKNAAMEMLJ_582", "locus": "GCA000027325_00559" }, { "type": "tRNA", "sequence": "contig_1", "start": 315301, "stop": 315377, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 73.0, "nt": "GCGCCCATAGCTCAATCGGATAGAGTGTCTGGCTTCGGACCAGAAGGTTATGGGTTCAAGTCCTATTGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 315360, 315362 ], "id": "KKNAAMEMLJ_31", "locus": "GCA000027325_00560" }, { "type": "cds", "sequence": "contig_1", "start": 315467, "stop": 315697, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_377", "db_xrefs": [ "RefSeq:WP_009885892.1", "SO:0001217", "UniParc:UPI0000557E10", "UniRef:UniRef100_UPI0000557E10", "UniRef:UniRef50_Q9EXD3", "UniRef:UniRef90_UPI0000557E10" ], "nt": "ATGGCAAAAGATAAAAAAAATAAGGTTGATGGAACAGAGCAATCAGTTGATCTATTTGAACGTACAAAACTTGAAGATACACAAGTTTTAAATGAAGTTGAACTTGATGATATTAAAAAGATAACAGAGCTTAGAAAAGAACTTGAACATACTTTTGAACCACAAACAAGAATGCAAATTAAGCGTGAAATTAAAGAAATAGAACGCAAAATGAAACGTTCTAGTCGCTAA", "aa": "MAKDKKNKVDGTEQSVDLFERTKLEDTQVLNEVELDDIKKITELRKELEHTFEPQTRMQIKREIKEIERKMKRSSR", "aa_hexdigest": "216d36a3184e8aa2b7fdf2f737d21396", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557E10", "ncbi_nrp_id": "WP_009885892.1", "uniref100_id": "UniRef100_UPI0000557E10", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557E10", "RefSeq:WP_009885892.1", "UniRef:UniRef100_UPI0000557E10" ] }, "ips": { "uniref100_id": "UniRef100_UPI0000557E10", "uniref90_id": "UniRef90_UPI0000557E10" }, "psc": { "uniref90_id": "UniRef90_UPI0000557E10", "uniref50_id": "UniRef50_Q9EXD3" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD3" ], "product": "Uncharacterized protein MPN_377" }, "genes": [], "id": "KKNAAMEMLJ_583", "locus": "GCA000027325_00561" }, { "type": "cds", "sequence": "contig_1", "start": 315701, "stop": 316063, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTAATTTACATACAAATTCATACTATAACTTTCTCAATTCTGCCCTTTCTCCTAAAAAGCTAGTTAATCTAGCAATTAATGATCAGCAAAAAGCTGTTGCTATTACAGATCCTAATCTTTTTGGCGCTGTTGAATTTTTTATAACTTGTAAGCAAAATAATATTAAACCAATTATTGGTTTAAACTTAACTGTTGAATACCAAAAAAATGATGTTAAGTTATTACTAATTGCTAAATCAAATAAAGGCTTTCAAACGTTGAACAAAATAGCATTAATTCAACAAAAACTTGAAATTAATTCTTTAGTTGATCAACTAACAGATATTGCAGTAATTATCTGTTCTTTAACAACATGA", "aa": "MFVNLHTNSYYNFLNSALSPKKLVNLAINDQQKAVAITDPNLFGAVEFFITCKQNNIKPIIGLNLTVEYQKNDVKLLLIAKSNKGFQTLNKIALIQQKLEINSLVDQLTDIAVIICSLTT", "aa_hexdigest": "a11942cdc52a670fb4f4b451b90467ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13410.536799999982, "isoelectric_point": 8.928629493713384 }, "id": "KKNAAMEMLJ_584", "locus": "GCA000027325_00562" }, { "type": "cds", "sequence": "contig_1", "start": 316196, "stop": 317308, "strand": "+", "frame": 2, "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "COG:COG0587", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0008408", "SO:0001217", "UniRef:UniRef50_Q49405", "UniRef:UniRef90_Q49405" ], "nt": "ATGAAACAAAACCAAACGGGAAAAATAACTACATTTGATTGGGATCTTAAACAAAAATTAAATCAAATTTCAATTAATGAAAATTTAAAAGTAAAGAGTGAAATTCAACCTTTTTTAGATCAAAAAACTGCACAACAATTATTCAGTGAAACAGAACTTAATAATCTGAATGATCTAGTTAATAGATGTGAATTAGATTTGGAGCACCTAAAAGCTGCTTCACTTTCTTTAACTGATAATGATGCAGCAGTTTTAGAAAGTTTGTGCCAAACCAATTTAAAACAGTTTTTAGATAAAAATCAAGATCTAAATAAAAAAGCCTATCAGCTACGTTTAGAGAAGGAATTAAATGTTATCAATAAACTTAATTTTGCTAGCTATTTTTTAGTTGTCAATGATCTTGTTAATTATGCTTTTAAAAAGGACATCTTAATTGGTTCTGGTAGAGGTTCTGCAGTAGGATCATTAGTGGCTTTTTTATTAAACATTACCAAGATAGACCCAGTCCAACACCAGCTTATTTTCGAACGTTTTATCTCAACCCACCGTCAAGATCTACCTGATATTGATATTGATATCATGGAGAATAAAAGAGCAGAAATGATAAATTATCTGTTTGAAAAATATGGCAAAGAAAACTGTGCACAAATTGTTACTTTTCAACGTTTTAAAACCCGTTCTGCTGTTAAAGAAGTTGCTAAATTATTTAATGATTATGGCATTAGTGACATGATCCTAGGAGTGTTACCTAAAGATCAAACTATAACATTCACTGATCTTAAAGCTACTGAAGATAGTGCTTTACAACTTTGTTTACAACAGTTTGGTTTAATTGTTGAATTAGCACTAGCAATAGTTGATTTTCCAAGACAATCAAGTATCCATGCTTCAGGCATAGTTATCGCTTCAAATTCTTTGATTAAAACCATTCCCTTGTTACAGCTTGACAATAATCACTTTTTAACTCAAGTTTCAATGGAATGGTTAAGTTTTTTTAATCTCAATAAGTTTGATCTGCTTGGTTTAATTAACCTTACTATGATTAGCGATGTAATTACCCAAATTAAACCATCTAACCAGACCGTTAACCAGTTTTTAAATACCATTTCTTGA", "aa": "MKQNQTGKITTFDWDLKQKLNQISINENLKVKSEIQPFLDQKTAQQLFSETELNNLNDLVNRCELDLEHLKAASLSLTDNDAAVLESLCQTNLKQFLDKNQDLNKKAYQLRLEKELNVINKLNFASYFLVVNDLVNYAFKKDILIGSGRGSAVGSLVAFLLNITKIDPVQHQLIFERFISTHRQDLPDIDIDIMENKRAEMINYLFEKYGKENCAQIVTFQRFKTRSAVKEVAKLFNDYGISDMILGVLPKDQTITFTDLKATEDSALQLCLQQFGLIVELALAIVDFPRQSSIHASGIVIASNSLIKTIPLLQLDNNHFLTQVSMEWLSFFNLNKFDLLGLINLTMISDVITQIKPSNQTVNQFLNTIS", "aa_hexdigest": "d61307d137169ed6e68e6e1617493579", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaE" ], "truncated": "both", "pseudogene": { "start": 315896, "stop": 317611, "observations": { "mutation": [ 316061, 317306 ], "stop": [ 316061, 317306 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49405", "query_cov": 1.0, "subject_cov": 0.4233409610983982, "identity": 1.0, "score": 710.0, "evalue": 1.91e-250, "gene_start": 1, "gene_end": 370, "reference_start": 166, "reference_end": 535, "reference_sequence": "MFVNLHTNSYYNFLNSALSPKKLVNLAINDQQKAVAITDPNLFGAVEFFITCKQNNIKPIIGLNLTVEYQKNDVKLLLIAKSNKGFQTLNKIALIQQKLEINSLVDQLTDIAVIICSLTTWKSTYKDVYQAKGIEINQTPIAILANAVNCEKTNSDQVVLTVLKQMKQNQTGKITTFDWDLKQKLNQISINENLKVKSEIQPFLDQKTAQQLFSETELNNLNDLVNRCELDLEHLKAASLSLTDNDAAVLESLCQTNLKQFLDKNQDLNKKAYQLRLEKELNVINKLNFASYFLVVNDLVNYAFKKDILIGSGRGSAVGSLVAFLLNITKIDPVQHQLIFERFISTHRQDLPDIDIDIMENKRAEMINYLFEKYGKENCAQIVTFQRFKTRSAVKEVAKLFNDYGISDMILGVLPKDQTITFTDLKATEDSALQLCLQQFGLIVELALAIVDFPRQSSIHASGIVIASNSLIKTIPLLQLDNNHFLTQVSMEWLSFFNLNKFDLLGLINLTMISDVITQIKPSNQTVNQFLNTISWTDQNTFINLVNEDTLGIFQLESFGMKKLLVQIKPKTINQLAIVLALYRPGAQDNINLFINRLHNGYDQSDIDPRILPIVKNTYGVLIFQEQIINIVKVVANYSLEEADSFRRAISKKDVKLIQKNKRNFFERAVQNNFDLKTTTKIFSYIERFANYGFNLSHALGYALLSYWTAWLKTNYPVYFYLWLLNHFQSSKDKQKLIIRTLEKSGIEIYPPLLNKAQPNSVIENKKIYLGLNLIKGINDRYIQNLQKVQHLIQTQNNLQLTDVVSWCLDKTIGDIPLKDLLLLKTMGCFDFFEYTYDFNDAKDFWIKSDHLLFTRMPLEKKDSNFWIKQFFTN" }, "paralog": false, "identity": 0.9964973730297724, "score": 1097.0, "evalue": 0.0, "description": "Internal stop codon at 316061, 317306. Nonsense mutation around 316061, 317306.", "psc": { "uniref90_id": "UniRef90_Q49405", "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q49405", "cog_id": "COG0587", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49405", "EC:2.7.7.7", "UniRef:UniRef50_Q49405", "COG:COG0587", "COG:L", "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q49405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49405" ], "product": "DNA polymerase III subunit alpha" } }, "id": "KKNAAMEMLJ_585", "locus": "GCA000027325_00563" }, { "type": "cds", "sequence": "contig_1", "start": 317381, "stop": 317824, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGAAAAAATTACTGGTTCAGATTAAACCTAAAACCATTAATCAACTAGCAATTGTTCTAGCGCTTTACAGACCAGGTGCACAGGATAACATTAACCTTTTTATTAACCGCTTGCACAATGGTTATGATCAATCTGACATTGATCCTAGGATTTTACCCATTGTGAAAAATACCTATGGAGTTTTAATTTTTCAAGAGCAGATCATTAACATCGTTAAAGTTGTGGCTAACTACTCTTTAGAAGAAGCAGATAGCTTCCGTAGAGCCATTTCTAAAAAGGATGTTAAATTGATCCAAAAAAATAAGCGTAACTTCTTTGAAAGAGCAGTTCAAAATAACTTTGATTTAAAGACTACTACCAAAATTTTTAGCTACATAGAACGCTTTGCTAACTATGGGTTTAACCTTTCTCATGCGTTGGGTTATGCACTGCTTTCATACTGA", "aa": "MKKLLVQIKPKTINQLAIVLALYRPGAQDNINLFINRLHNGYDQSDIDPRILPIVKNTYGVLIFQEQIINIVKVVANYSLEEADSFRRAISKKDVKLIQKNKRNFFERAVQNNFDLKTTTKIFSYIERFANYGFNLSHALGYALLSY", "aa_hexdigest": "a1a9a9b13fbce59c5aa0dabbc9c84b39", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 115, "aa_cov": 0.7687074829931972, "hmm_cov": 1.0, "evalue": 1.1930691937180525e-36, "score": 120.6449966430664, "start": 1, "stop": 113 } ], "seq_stats": { "molecular_weight": 17055.596099999988, "isoelectric_point": 9.785091972351072 }, "id": "KKNAAMEMLJ_586", "locus": "GCA000027325_00564" }, { "type": "cds", "sequence": "contig_1", "start": 318065, "stop": 318238, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAACATTTAATTCAAACTCAAAATAACTTACAACTAACTGATGTAGTAAGTTGGTGTTTGGATAAAACCATTGGTGATATCCCTTTAAAAGATTTACTTTTATTAAAAACTATGGGCTGTTTTGATTTTTTTGAATACACTTATGACTTTAATGATGCAAAGGATTTTTGA", "aa": "MQHLIQTQNNLQLTDVVSWCLDKTIGDIPLKDLLLLKTMGCFDFFEYTYDFNDAKDF", "aa_hexdigest": "69c2fb9a3f3b43dc08c1018e39d14ff5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6720.630399999998, "isoelectric_point": 4.222591590881348 }, "id": "KKNAAMEMLJ_587", "locus": "GCA000027325_00565" }, { "type": "cds", "sequence": "contig_1", "start": 318452, "stop": 319207, "strand": "+", "frame": 2, "gene": "polA", "product": "5'-3' exonuclease", "db_xrefs": [ "COG:COG0258", "COG:L", "EC:3.1.11.-", "GO:0003677", "GO:0008409", "GO:0017108", "GO:0033567", "SO:0001217", "UniRef:UniRef50_P75403", "UniRef:UniRef90_Q49406" ], "nt": "ATGTTACTAATGTGTTGGAATTTAATTAAAGCCAATGTTTATCAATATGGAATTGTAAGTTTTGACACTAAAGCGCCAACTTTCCGTGATCAAATCTATGAAGGATATAAACAAAAAAGGGTTAAAACTCCAGTTGAACTTTTAGTACAAATTCCTCTAATTAAACAAGCGCTTGTTTATTTAGGGTTTTTAGTTTGTGAAAAAGATGGCTTTGAAGCAGATGATTTGATTGGTAGTTATGCCAATTTATTTACAAAGCAAGAAATAACAGTTGATATTTACAGTTCAGATCGAGATATGTTGCAATTAGTAAACGCCTTTACTAATGTGTTTCTCTGTATTAAAGGTACAAAGGAGATGGTTATGTACAACAATGAAAATTTCAAATCACTTTTTTATGGTTTGGCGCCCTATCAAGTTGTTGAATATAAGGGGTTAGTTGGTGATAACAGCGATAATTTAGCAGGGATTAAAGGGATAGGTCCCATCAAAGGGATAGAATTACTCCAACAATATGGAACCATTGATAACATCTACACTAACTTCAATAATCTCCCCAACCAACTTCAAAAACTTTTAAATAACCAAAAGGAAATAGCTAAAACCTTTAGTTTTCTAGCTAAAATTAAAACTGATATTGAACTTGATCAAAACATAGATCTTACTGGTTTAAAACCAATCCAAAAACAAGCGTTAATTCAACTTCTAAGTGAAAACAAAATTAATACTTTAGTTGAAAAATTTTCAAAAATATAA", "aa": "MLLMCWNLIKANVYQYGIVSFDTKAPTFRDQIYEGYKQKRVKTPVELLVQIPLIKQALVYLGFLVCEKDGFEADDLIGSYANLFTKQEITVDIYSSDRDMLQLVNAFTNVFLCIKGTKEMVMYNNENFKSLFYGLAPYQVVEYKGLVGDNSDNLAGIKGIGPIKGIELLQQYGTIDNIYTNFNNLPNQLQKLLNNQKEIAKTFSFLAKIKTDIELDQNIDLTGLKPIQKQALIQLLSENKINTLVEKFSKI", "aa_hexdigest": "8dc861eb7a7681448c5d67fb0955a94d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49406", "query_cov": 1.0, "subject_cov": 0.8625429553264605, "identity": 1.0, "score": 495.0, "evalue": 2.38e-176, "valid": true, "gene": "polA", "product": "5'-3' exonuclease", "ec_ids": [ "3.1.11.-" ], "uniref50_id": "UniRef50_P75403", "cog_id": "COG0258", "cog_category": "L", "go_ids": [ "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ] }, "pscc": { "uniref50_id": "UniRef50_P75403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75403" ], "product": "5'-3' exonuclease" }, "genes": [ "polA" ], "id": "KKNAAMEMLJ_588", "locus": "GCA000027325_00566" }, { "type": "cds", "sequence": "contig_1", "start": 319207, "stop": 320061, "strand": "+", "frame": 1, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "COG:COG0266", "COG:L", "EC:3.2.2.23", "GO:0003684", "GO:0003906", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078", "KEGG:K10563", "RefSeq:WP_010869400.1", "SO:0001217", "UniParc:UPI000012AB8B", "UniRef:UniRef100_P55825", "UniRef:UniRef50_P55825", "UniRef:UniRef90_P55825" ], "nt": "ATGCCTGAACTTCCTGAAGTAACTACTGTTATTAATGAACTTAAAGAAACTGTTTTAAATAAACCTTTAGATCAAGTTCAAGTTAACCTAAGAAAGGTTTTGAAAAATATTGATCCTCAATTGCTGAATAAACAATTAAAAAATCAGTTTTTTACTGATATTAAGCGTAAGGGTAAATATATCATTTTTCTTTTAAGTAATGGTTTGTATTTAGTTTCGCATTTACGTATGGAAGGTAAATACTTTTTTGAAGAAAGAGGTAGTAAATTTAATCAAAAGCATGTTTTAGTAGAATTTCATTTTGATGATGGTAGTCAACTCAATTATCATGACACCAGACAATTTGGAACGTTCCATTTGTATGAAAAGTTAGAACAAGCAGCACAATTAAATAAACTTGCATTTGATCCTCTAGAAGCTGGTTTTGACTATAGGAAAATCTTCCAAAAAGCACAAAATTCAAAACGTAAAGTTAAAACTTTTATTTTAGACCAAACAGTGATTAGTGGAATTGGCAATATTTATGCAGATGAAATCTTATTTGCAAGCAAAATTAATCCTGAAACAATGGTTGATCAACTAACAATTAAAGAGATAGAGATTTTATGTAAAAATGCTACCAAAATTTTAGCTAAAGCAATAGTTATGAAAGGTACTACCATCAGCAGCTTTAGTTTTAAAAAAGATCATACTGGAGGCTATCAAAACTTTTTAAAAGTTCACACTAAAAAAGATCAACCTTGCTCAGTTTGTAACCAATTAATTGTTAAAAAGAAGATTAATGGAAGGGGGAGCTATTTTTGTTTAAACTGTCAAAAAATCACAACCAAAGTTTCTACAAAACTCAATCCATAA", "aa": "MPELPEVTTVINELKETVLNKPLDQVQVNLRKVLKNIDPQLLNKQLKNQFFTDIKRKGKYIIFLLSNGLYLVSHLRMEGKYFFEERGSKFNQKHVLVEFHFDDGSQLNYHDTRQFGTFHLYEKLEQAAQLNKLAFDPLEAGFDYRKIFQKAQNSKRKVKTFILDQTVISGIGNIYADEILFASKINPETMVDQLTIKEIEILCKNATKILAKAIVMKGTTISSFSFKKDHTGGYQNFLKVHTKKDQPCSVCNQLIVKKKINGRGSYFCLNCQKITTKVSTKLNP", "aa_hexdigest": "bbd5555ec735efd141f97aacfaa496b8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012AB8B", "ncbi_nrp_id": "WP_010869400.1", "uniref100_id": "UniRef100_P55825", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012AB8B", "RefSeq:WP_010869400.1", "UniRef:UniRef100_P55825" ] }, "ips": { "uniref100_id": "UniRef100_P55825", "uniref90_id": "UniRef90_P55825" }, "psc": { "uniref90_id": "UniRef90_P55825", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23" ], "uniref50_id": "UniRef50_P55825", "cog_id": "COG0266", "cog_category": "L", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003684", "GO:0003906", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ] }, "pscc": { "uniref50_id": "UniRef50_P55825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55825" ], "product": "Formamidopyrimidine-DNA glycosylase" }, "genes": [ "mutM" ], "id": "KKNAAMEMLJ_589", "locus": "GCA000027325_00567" }, { "type": "cds", "sequence": "contig_1", "start": 320408, "stop": 320554, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTACCATTACTTTAGATGGGGCTATTATCTTTTATCAAAACGAAATCTTAAGTCAAACTTATTTAGATGATAGATTTATTGAAGGGATTAATAACATAGTAAAGCGCTTTCCTGAAGCTGCTTATAAACTTAACAGTGGTTGA", "aa": "MPTITLDGAIIFYQNEILSQTYLDDRFIEGINNIVKRFPEAAYKLNSG", "aa_hexdigest": "f20adc33eaa4cc4f8bdb9c3ebc48e9ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5494.1902, "isoelectric_point": 4.593750190734863 }, "id": "KKNAAMEMLJ_590", "locus": "GCA000027325_00568" }, { "type": "cds", "sequence": "contig_1", "start": 320732, "stop": 320929, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGCAAAAGGAAATAATTGAACTAATTAGTGTTAGTGATGTAAATGCCAAAATCTATGAGCACTCTATGTACATTATTAAAAATGGTGTTTCTAAAGCTAGTGCATTGCAAAGCCTTAACCAGTTTGCAATTCCAATAACAAAAGATAACACTATTGTTTGTGGGGATGGAGATAATGACATTGAAATGATGCAGTGA", "aa": "MQKEIIELISVSDVNAKIYEHSMYIIKNGVSKASALQSLNQFAIPITKDNTIVCGDGDNDIEMMQ", "aa_hexdigest": "d47c9668724ae7cabc2cd0138eac8d98", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 50, "aa_cov": 0.7692307692307693, "hmm_cov": 0.98, "evalue": 3.261159829224751e-11, "score": 38.46260070800781, "start": 16, "stop": 65 } ], "seq_stats": { "molecular_weight": 7217.211100000002, "isoelectric_point": 4.5550428390502935 }, "id": "KKNAAMEMLJ_591", "locus": "GCA000027325_00569" }, { "type": "cds", "sequence": "contig_1", "start": 321046, "stop": 321324, "strand": "+", "frame": 1, "gene": "coaE", "product": "dephospho-CoA kinase", "db_xrefs": [ "COG:COG0237", "COG:H", "EC:2.7.1.24", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937", "KEGG:K00859", "SO:0001217", "UniRef:UniRef50_P47506", "UniRef:UniRef90_P47506" ], "nt": "ATGTTAATTGCAATCGTAGGTAAACCAGGTGTTGGTAAAACCAGTCTATTGCAATATCTCAAAGATAACTATCACTTTTCAGTTTTTTATGCAGATAGTTTTATCCATGAACAGTACCAAAAAAACAATCCAGGTTATCAATTAATCATGGATCATTTTGGCAAAGAGTTTGTCAATCAAACTGAAGTTGATCGTAAAAAACTAGCAAACTATGTTTTTAGTGATGATAAGTTAATCGAAAAACTTTCACTAGTAACAAAACCGCTGTTAATAGCGTGA", "aa": "MLIAIVGKPGVGKTSLLQYLKDNYHFSVFYADSFIHEQYQKNNPGYQLIMDHFGKEFVNQTEVDRKKLANYVFSDDKLIEKLSLVTKPLLIA", "aa_hexdigest": "451dec50d2b01e479ee19b450e831152", "start_type": "ATG", "rbs_motif": null, "genes": [ "coaE" ], "truncated": "3-prime", "pseudogene": { "start": 321046, "stop": 321627, "observations": { "mutation": [ 321322, 321394 ], "stop": [ 321322, 321394 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47506", "query_cov": 1.0, "subject_cov": 0.46464646464646464, "identity": 1.0, "score": 185.0, "evalue": 5.69e-58, "gene_start": 1, "gene_end": 92, "reference_start": 1, "reference_end": 92, "reference_sequence": "MLIAIVGKPGVGKTSLLQYLKDNYHFSVFYADSFIHEQYQKNNPGYQLIMDHFGKEFVNQTEVDRKKLANYVFSDDKLIEKLSLVTKPLLIAWIKSLKTQFQKKLALIEIAVMLNYWNEYRSLFDYVIKLERDDQLVNLALQQRNSHKKVKDLIKEPNCKIDTIFNNDSIATAALKLIKLLETFLERNKCRCDCCHIQ" }, "paralog": false, "identity": 0.9896373056994818, "score": 377.0, "evalue": 4.37e-136, "description": "Internal stop codon at 321322, 321394. Nonsense mutation around 321322, 321394.", "psc": { "uniref90_id": "UniRef90_P47506", "gene": "coaE", "product": "dephospho-CoA kinase", "ec_ids": [ "2.7.1.24" ], "uniref50_id": "UniRef50_P47506", "cog_id": "COG0237", "cog_category": "H", "kegg_orthology_id": "K00859", "go_ids": [ "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47506", "EC:2.7.1.24", "UniRef:UniRef50_P47506", "COG:COG0237", "COG:H", "KEGG:K00859", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ] }, "pscc": { "uniref50_id": "UniRef50_P47506", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47506" ], "product": "Dephospho-CoA kinase" } }, "id": "KKNAAMEMLJ_592", "locus": "GCA000027325_00570" }, { "type": "cds", "sequence": "contig_1", "start": 321424, "stop": 321642, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAATTAGAAAGGGATGATCAGCTAGTTAACTTAGCTTTACAACAACGTAATAGTCATAAAAAAGTTAAGGATTTGATTAAAGAGCCTAATTGCAAAATAGATACAATTTTCAACAACGATTCGATTGCAACAGCTGCTTTAAAGCTAATTAAGTTGCTAGAAACTTTTTTAGAAAGAAATAAATGCCGTTGTGATTGTTGTCATATTCAGTAA", "aa": "MIKLERDDQLVNLALQQRNSHKKVKDLIKEPNCKIDTIFNNDSIATAALKLIKLLETFLERNKCRCDCCHIQ", "aa_hexdigest": "dff1722a4cb657f92f8c5d87688485c6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8396.792599999993, "isoelectric_point": 8.678105354309082 }, "id": "KKNAAMEMLJ_593", "locus": "GCA000027325_00571" }, { "type": "cds", "sequence": "contig_1", "start": 321578, "stop": 322414, "strand": "-", "frame": 2, "gene": "cof", "product": "Putative phosphatase MG265", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "RefSeq:WP_009885896.1", "SO:0001217", "UniParc:UPI0000139535", "UniRef:UniRef100_P47507", "UniRef:UniRef50_P75399", "UniRef:UniRef90_P47507" ], "nt": "ATGGAACTGAAAAACATTATTTTTGACCTTGATGGTACCTTGCTTTCAAGCAACCAAATTCCATTAGAACAAACAGTTGAGTTTTTAAAGGATTTACAGAAAAAAGGGATTAGAATCACTTTTGCTAGTGGTAGAAGCCATATTTTAATTAGAAACACAGCTACCTTTATTACACCAAATCTACCTGTAATTTCTTCCAATGGTGCACTTGTTTATGATTTTGCTAGTGAAAAACCAGTTCATATCAAACCTATTGATAATAAAGTAATACCTGCAATTATGCAAATGTTGTTGGAATTTCAAGAAACATTTTATTTCTATACAGATAAAAAGGTTTTTGCTTTTACACATGAGCTTGATTCAGCTAAAATTCTTTCAACTAGAAGTCAAATAGTAGGAATTGATCTCATTGAAAATAACTACATAGTTAACAAGTTTGAAAAAGCTTTGGATTTTGATTTTAAGCAACATACTATTACAAAGATCTTACTGGTAACTAAAAACAGAGAAAAAGTTCCTTTTCTAGCAAAACAACTAGATCAAATTCAAGATATTAACTATGTGAGTTCAATGACATTTGCTCTTGATATCATGCAAAAAGATGTTAATAAGGCTTATGGATTGAAAGTATTAGTTGATAATTATAATCTTGATCCTGAAAAGACTATGGTCTTTGGTGATGCTGATAATGATGTTGAAATATTTCAAAGTGTTAAATGGCCAGTTGCTTTGGTTAATGGCACTGATTTAGCTAAAAAAAATGCTAAGTTTATTACTGAATATGACAACAATCACAACGGCATTTATTTCTTTCTAAAAAAGTTTCTAGCAACTTAA", "aa": "MELKNIIFDLDGTLLSSNQIPLEQTVEFLKDLQKKGIRITFASGRSHILIRNTATFITPNLPVISSNGALVYDFASEKPVHIKPIDNKVIPAIMQMLLEFQETFYFYTDKKVFAFTHELDSAKILSTRSQIVGIDLIENNYIVNKFEKALDFDFKQHTITKILLVTKNREKVPFLAKQLDQIQDINYVSSMTFALDIMQKDVNKAYGLKVLVDNYNLDPEKTMVFGDADNDVEIFQSVKWPVALVNGTDLAKKNAKFITEYDNNHNGIYFFLKKFLAT", "aa_hexdigest": "a8175bd90a9b8fe365110442e827d3a1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139535", "ncbi_nrp_id": "WP_009885896.1", "uniref100_id": "UniRef100_P47507", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139535", "RefSeq:WP_009885896.1", "UniRef:UniRef100_P47507" ] }, "ips": { "uniref100_id": "UniRef100_P47507", "uniref90_id": "UniRef90_P47507" }, "psc": { "uniref90_id": "UniRef90_P47507", "gene": "Cof", "product": "Putative phosphatase MG265", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P75399", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P75399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75399" ], "product": "Putative phosphatase MPN_383" }, "genes": [ "cof" ], "id": "KKNAAMEMLJ_594", "locus": "GCA000027325_00572" }, { "type": "cds", "sequence": "contig_1", "start": 322433, "stop": 322954, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "TTGGATCGAGTTTATAACTTCTTTTTTAATCATGCTGTTGTTACTGATCAAGTTAGTCAAGAGACAATCTTTGCTTACAATTTGTTTTTAAAAAACAGTTATTGTCATCTTGACAAACATGAACTAAATCTGGTGATTAGTGAAATGATGATCTTTTTAAACTTTCTCTATAAAACCAAAAAAATTAGCTTAAATTATGCAAAGGGATTTTTAACAGTACTGTCGTTTTTTGCGCCCTTTCTTGCTGAAGAATTGAATGAAAAATGTGGACTTGAACCATTTGTTGTTAAACAAGCGATTTCTTTAGTTGATTATCAACTTTTTGAGACTGCTAAAACTAAGGTTATTCTTTCAATTAATGGCAAATTTAAAGCAGCTAAAGAATTTACTAAAGGTAGTTTAGAGATAGATGTTTTAGAATCATTTAAACAGGATAAAGAGATAAATGACATTCTCAACCAACCGATTGAGAGGGTAGTTTATGTTCAGGATCGAATTATTAATGTTCTTTTAAAAAAATAG", "aa": "MDRVYNFFFNHAVVTDQVSQETIFAYNLFLKNSYCHLDKHELNLVISEMMIFLNFLYKTKKISLNYAKGFLTVLSFFAPFLAEELNEKCGLEPFVVKQAISLVDYQLFETAKTKVILSINGKFKAAKEFTKGSLEIDVLESFKQDKEINDILNQPIERVVYVQDRIINVLLKK", "aa_hexdigest": "ffadb7354b04611047220b9ebfa5465f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 132, "aa_cov": 0.6011560693641619, "hmm_cov": 0.9924242424242424, "evalue": 5.2267500939696805e-09, "score": 31.2218074798584, "start": 29, "stop": 132 } ], "seq_stats": { "molecular_weight": 20152.2738, "isoelectric_point": 6.833490562438965 }, "id": "KKNAAMEMLJ_595", "locus": "GCA000027325_00573" }, { "type": "cds", "sequence": "contig_1", "start": 322988, "stop": 323191, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGTTTGACAAGAAGCTAGTATCAACAAAAGAACCATTTCAAAAATTAATTAATCAGGGTATGGTGTTAGGTCCTGATGGTAAAAAGATGTCCAAATCCAAAGGTAATACCATTAACCCCACACCACTTGTTGATTCACATGGAGCAGATGCTTTAAGGTTGTACTTAATGTTTATGGGCCCAATTAGTGCTAGTTTAACTTGA", "aa": "MFDKKLVSTKEPFQKLINQGMVLGPDGKKMSKSKGNTINPTPLVDSHGADALRLYLMFMGPISASLT", "aa_hexdigest": "eb115c2118611b228a8c3c32605c2634", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 52, "aa_cov": 0.7761194029850746, "hmm_cov": 0.9807692307692307, "evalue": 1.92561543725279e-17, "score": 58.140174865722656, "start": 8, "stop": 59 } ], "seq_stats": { "molecular_weight": 7295.5466000000015, "isoelectric_point": 9.698381996154787 }, "id": "KKNAAMEMLJ_596", "locus": "GCA000027325_00574" }, { "type": "cds", "sequence": "contig_1", "start": 323372, "stop": 323860, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF13603.12" ], "nt": "TTGATCCCTGTCTATGTTGCTAACTATGTAATTGAAGGATATGGAACAGATGCTATTATGGGTGTTGGAGCACACAATGAAAATGATAACTTCTTCGCACGTAAACAAAAGTTGAAAATTATCAACGTCATTGATAAAAAAGAACGGCTGCAAAATTCATTTGCATATAACGGATTAACAACTAAAGAAGCACAAGTAGCTATTACTAATGAGTTAATTTCACAAAATAAAGCGAAATTAACAACTGTATATAAACTGCGTGATTGGATCTTCAGTAGACAGCGTTATTGGGGCGAACCTTTTCCAATTATTTTTGATGAAAATAACACTCCTCATTTGGTAGAACAACTCCCTGTTGAATTACCCTTACTTGAGAATTACAAACCAGATGGAAGTGGTAATTCTCCACTAATGAGAAATCAAGCTTGGGTAAACATAGTCAAAGATAACATCCATTACCAAAGGGAAACTAATACCATGCCCCAATGA", "aa": "MIPVYVANYVIEGYGTDAIMGVGAHNENDNFFARKQKLKIINVIDKKERLQNSFAYNGLTTKEAQVAITNELISQNKAKLTTVYKLRDWIFSRQRYWGEPFPIIFDENNTPHLVEQLPVELPLLENYKPDGSGNSPLMRNQAWVNIVKDNIHYQRETNTMPQ", "aa_hexdigest": "6d1f30fdb06f2ff605d0193935d50714", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 37, "aa_cov": 0.22839506172839505, "hmm_cov": 1.0, "evalue": 2.2479128662753992e-07, "score": 24.979543685913086, "start": 75, "stop": 111 }, { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 57, "aa_cov": 0.345679012345679, "hmm_cov": 0.9824561403508771, "evalue": 5.679193164417119e-18, "score": 60.13599395751953, "start": 2, "stop": 57 } ], "seq_stats": { "molecular_weight": 18758.094199999996, "isoelectric_point": 6.838776588439941 }, "id": "KKNAAMEMLJ_597", "locus": "GCA000027325_00575" }, { "type": "cds", "sequence": "contig_1", "start": 324518, "stop": 324703, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTCCCTTATCCCTCAGCAGCAGGATTACATTTAGGACATGTTAGAGCTTATACTATCACTGATGTAATAAGTAGGTATTACAAAGCTAAAGGATTTAATGTGATCCATCCGATTGGTTTTGATGCTTTTGGTTTACCTGCTGAACAGTATGCTATTAACTCTAATCAAAACCCTGGCAGTTGA", "aa": "MFPYPSAAGLHLGHVRAYTITDVISRYYKAKGFNVIHPIGFDAFGLPAEQYAINSNQNPGS", "aa_hexdigest": "92924fc03ef03d109b9b91edf6dabae5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.7540983606557377, "hmm_cov": 1.0, "evalue": 8.871281619057602e-10, "score": 32.897457122802734, "start": 5, "stop": 50 } ], "seq_stats": { "molecular_weight": 6684.463900000002, "isoelectric_point": 8.122839164733886 }, "id": "KKNAAMEMLJ_598", "locus": "GCA000027325_00576" }, { "type": "cds", "sequence": "contig_1", "start": 324812, "stop": 324934, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCACTTTTTTATCCCTGTTGGCTTTGGGTTGTTATTTGGTTTGATTATTGCGATTATTGTTGACATACTCTATCGCTTAACTAAATATGCAATCAAGCGTTCTTATCAAAGCAATTAG", "aa": "MVHFFIPVGFGLLFGLIIAIIVDILYRLTKYAIKRSYQSN", "aa_hexdigest": "57dde39cf5d183e04b35dde3385cf6f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4631.5682000000015, "isoelectric_point": 9.819775962829588 }, "id": "KKNAAMEMLJ_599", "locus": "GCA000027325_00577" }, { "type": "cds", "sequence": "contig_1", "start": 325205, "stop": 325405, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAACCGCAAAGTAGAGATTGATAACTTTACTAAAAATCAACTTTACTTTCAAAATTTACACAGGGTTTACACTGGATTTATGGAAGCGGTTTGTAATGATTTTGGGATTAATTACTGTATTATAGATGCAAAACTACCAATAGTAACTATTATTAAAATGATCCTTGAAAAATTAAAGTTACAAAAGTTAGATTGA", "aa": "MRNRKVEIDNFTKNQLYFQNLHRVYTGFMEAVCNDFGINYCIIDAKLPIVTIIKMILEKLKLQKLD", "aa_hexdigest": "91b11843a60bdb49a0903a8ab9ca84aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7866.274499999997, "isoelectric_point": 9.031456565856931 }, "id": "KKNAAMEMLJ_600", "locus": "GCA000027325_00578" }, { "type": "cds", "sequence": "contig_1", "start": 325502, "stop": 325879, "strand": "-", "frame": 2, "gene": "dck", "product": "putative protein MG268", "db_xrefs": [ "COG:COG1428", "COG:F", "GO:0005524", "GO:0005737", "GO:0019136", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_P47510" ], "nt": "ATGCAACTAAAAAAGCCCCATTTTCAACCAAATAAAATTGCTAATTGTATTGTGATCGGGGGAATGATTGCTTTAGGAAAAACCACCATTGCTAATACATTAGCTAACCACATTCAAGCTGCAAAAGTTGTTTGTGAATTGGAAACTAATGACCAGTTGGTTGAACTTTTACTAGCAAAGATGTATGAACGTAGTGATGAATTGCTCTATTCACCTTTGTTTCAGCTTTATTTTACGCTTAATCGCTTTGGTAAATACCAGAACAATTGCAACACTATCAATCCAACCATTTTTGATCGTTCTATCTTTGAAGACTGGTTGTTTGCTAAGCACAACATCATTCGTCCTGCAGTCTTTTCATACTATAACCAACTGTGA", "aa": "MQLKKPHFQPNKIANCIVIGGMIALGKTTIANTLANHIQAAKVVCELETNDQLVELLLAKMYERSDELLYSPLFQLYFTLNRFGKYQNNCNTINPTIFDRSIFEDWLFAKHNIIRPAVFSYYNQL", "aa_hexdigest": "688c1b80249a21e529d64277cb92260d", "start_type": "ATG", "rbs_motif": null, "genes": [ "dck" ], "truncated": "5-prime", "pseudogene": { "start": 325199, "stop": 325879, "observations": { "mutation": [ 325207, 325432, 325504 ], "stop": [ 325207, 325432, 325504 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47510", "query_cov": 1.0, "subject_cov": 0.5482456140350878, "identity": 1.0, "score": 258.0, "evalue": 5.15e-86, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MQLKKPHFQPNKIANCIVIGGMIALGKTTIANTLANHIQAAKVVCELETNDQLVELLLAKMYERSDELLYSPLFQLYFTLNRFGKYQNNCNTINPTIFDRSIFEDWLFAKHNIIRPAVFSYYNQLWNRLAKELVNKHGVPNLYVILDGDWKLFEKRLFMRNRKVEIDNFTKNQLYFQNLHRVYTGFMEAVCNDFGINYCIIDAKLPIVTIIKMILEKLKLQKLDWKFI" }, "paralog": false, "identity": 0.9867256637168141, "score": 449.0, "evalue": 1.08e-163, "description": "Internal stop codon at 325207, 325432, 325504. Nonsense mutation around 325207, 325432, 325504.", "psc": { "uniref90_id": "UniRef90_P47510", "gene": "Dck", "product": "Uncharacterized protein MG268", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0019136" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47510", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F", "GO:0005737", "GO:0005524", "GO:0019136" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "KKNAAMEMLJ_601", "locus": "GCA000027325_00579" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 325915, "stop": 326010, "strand": "-", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 61.6, "evalue": 5.5e-08, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "ATTTAGAACCATCACATCATTAGAGTCGAATCATGTCAGGCCAGAAATGGAGCAGCATTAAGACTTGTCAGTGAGTGTGATGGTTTTTTATTAAAA", "id": "KKNAAMEMLJ_42", "locus": "GCA000027325_00580" }, { "type": "cds", "sequence": "contig_1", "start": 326030, "stop": 327052, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG269", "db_xrefs": [ "RefSeq:WP_009885900.1", "SO:0001217", "UniParc:UPI0000139545", "UniRef:UniRef100_Q49407", "UniRef:UniRef50_Q49407", "UniRef:UniRef90_Q49407" ], "nt": "ATGAATAATTTTGATTACTATAGAGACTTTGATGACTTTCAAAGAAGAGAAACTATTAACTTCTTTTTAGCCAAGTTTCCCTTTGCTTCCAAAGCCCAGCTTGATGTTTTTTTAGAACAAGCAAAAACTGCTTATGTCAAACTACGAACAAGCAACCCAAAAAATGTTGATTGGAACCAGATGCTTGTTTTGTTATTAAAAAAGTTTGGTCCTGAAGCGAGAAGTGAAAAAGAACGTTTAAAAAGGATCTTAAGTTTGCAAGAACAACTTAAGGTACGCTTGGAAGGAGAGATTAACCGTCAAGTTCAACAGAACAGCGAACTTTTCTCACAACTCAAACAAAGTGAAAGTGAAATTATCCAAATGCAGCAGCTGGTAGAAGCAAAAGAACACCAAATTGAAGCATTAAATAAACAGTTACATGCAATAAAAGAAGCCAATAAGAAACTAATTGAAGAACATGAAAGTATTAATGTAGAGGAATTGTTAAAGGAATATGAAGTGCAGTGTAATGAAGCAATTTATAAACGTGATCAACACATCCAAACCGTATTTGAAGACAAGTTAGCACTTAAAGATGGGGAAATTAGTGAAACCCAATCACTTTTAAAAACCGCTGAAAAAGAAAAGCAAGCATTAAAAAAAGCATATAAGTTAGTTGTAAATTCGCTTCAAAAACACCAAAAGCTCACCACCGAAATAGAAATTGATTTCACCAAGCTTGATGAGATTATTGCAACCATTTTTGATGAAACTAAGAATCCAAAGACTGGCTTTACTAACTTCATTAAGCAGTTTGAAAAAACCAAAGCAAAACTAACAAAAAAGATAGCTGAAATTACTAAACTTGATCATTCAACGCCAACAAATTATCAACAAGAAACTCCAGCGTCACAACAGCAATTAGATCAAGAGAATGAACCGATCAAACCATCTAAAAAATCAAATTCAAGTTCATTGCCTAGAGGCACTACACAACCCAAATCCAATTCTATTAACAGGGTTAGTAAATTAATTGATTAA", "aa": "MNNFDYYRDFDDFQRRETINFFLAKFPFASKAQLDVFLEQAKTAYVKLRTSNPKNVDWNQMLVLLLKKFGPEARSEKERLKRILSLQEQLKVRLEGEINRQVQQNSELFSQLKQSESEIIQMQQLVEAKEHQIEALNKQLHAIKEANKKLIEEHESINVEELLKEYEVQCNEAIYKRDQHIQTVFEDKLALKDGEISETQSLLKTAEKEKQALKKAYKLVVNSLQKHQKLTTEIEIDFTKLDEIIATIFDETKNPKTGFTNFIKQFEKTKAKLTKKIAEITKLDHSTPTNYQQETPASQQQLDQENEPIKPSKKSNSSSLPRGTTQPKSNSINRVSKLID", "aa_hexdigest": "3a06001d4ec238cc99db913137ad725a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139545", "ncbi_nrp_id": "WP_009885900.1", "uniref100_id": "UniRef100_Q49407", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139545", "RefSeq:WP_009885900.1", "UniRef:UniRef100_Q49407" ] }, "ips": { "uniref100_id": "UniRef100_Q49407", "uniref90_id": "UniRef90_Q49407" }, "psc": { "uniref90_id": "UniRef90_Q49407", "product": "Uncharacterized protein MG269", "uniref50_id": "UniRef50_Q49407" }, "pscc": { "uniref50_id": "UniRef50_Q49407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49407" ], "product": "Uncharacterized protein MG269" }, "genes": [], "id": "KKNAAMEMLJ_602", "locus": "GCA000027325_00581" }, { "type": "cds", "sequence": "contig_1", "start": 327045, "stop": 327431, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG269-1", "db_xrefs": [ "RefSeq:WP_009885901.1", "SO:0001217", "UniParc:UPI0000557E21", "UniRef:UniRef100_Q49329", "UniRef:UniRef50_Q49329", "UniRef:UniRef90_Q49329" ], "nt": "ATGGATAGTTTTGTTCAAAAATACACAAATGGCTTCAAATCAATTTTGAACAAGGTTGAAAAAACTGATTTTGCAACTATTAAAAGTGAATTTCAATATAACCAAGCTAACTTGGAATGGGTTGAAAGTAAAGTTAGTGATCTTAACAACTATTTACTAGATCCTAACCAGTTTAGTGATGTAGTTAGTTTTAAAAAAATTGCTAATGAAAAGTTAGATTTGTTTGTTAAAAACCATGGCAATAAACTCCCGTTTTTTCTCTTTACTAGTTTTGTTTTAGCAATCTTTAGTTTTGTTAGTGTTTATGTTAGACACCATTATGATTTAGACTTTAATGATCCTGATGCTATCATTAGCTTCTTTAGAGAATTAGCTTTCCATGAATAA", "aa": "MDSFVQKYTNGFKSILNKVEKTDFATIKSEFQYNQANLEWVESKVSDLNNYLLDPNQFSDVVSFKKIANEKLDLFVKNHGNKLPFFLFTSFVLAIFSFVSVYVRHHYDLDFNDPDAIISFFRELAFHE", "aa_hexdigest": "1146b1a58ef06b100147b7ea7666ba20", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557E21", "ncbi_nrp_id": "WP_009885901.1", "uniref100_id": "UniRef100_Q49329", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557E21", "RefSeq:WP_009885901.1", "UniRef:UniRef100_Q49329" ] }, "ips": { "uniref100_id": "UniRef100_Q49329", "uniref90_id": "UniRef90_Q49329" }, "psc": { "uniref90_id": "UniRef90_Q49329", "product": "Uncharacterized protein MG269.1", "uniref50_id": "UniRef50_Q49329" }, "pscc": { "uniref50_id": "UniRef50_Q49329", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49329" ], "product": "Uncharacterized protein MG269.1" }, "genes": [], "id": "KKNAAMEMLJ_603", "locus": "GCA000027325_00582" }, { "type": "cds", "sequence": "contig_1", "start": 327721, "stop": 327831, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTTTTCACTGTTACTGAAAAAGCAGAAACAATTGTTTTAACTAAAGATGCACTAGCAAAGGTTGAAAAAAGAGCAAAAGAACACTTTCAATCATGGGAGTGA", "aa": "MINFFTVTEKAETIVLTKDALAKVEKRAKEHFQSWE", "aa_hexdigest": "cd5d4224ba381beb8927f39d7f8f2154", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4239.8462, "isoelectric_point": 6.556798362731934 }, "id": "KKNAAMEMLJ_604", "locus": "GCA000027325_00583" }, { "type": "cds", "sequence": "contig_1", "start": 327856, "stop": 328377, "strand": "-", "frame": 3, "gene": null, "product": "biotin/lipoate A/B protein ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0037DCED81" ], "nt": "TTGCTAACTGAATTTAGAAAAAATGAGTTAGTTAAGGTCATCTACTTTTGGCAGAACGCTAACACTATTGTGGTGGGAAGAAACCAAAATACTTATGCTGAGGTTAACTTAAAGGAGTTGGAAAGTGATAAGGTTAACTTGTTTAGACGTTTTTCAGGCGGGGGAGCGGTGTTTCATGACCTTGGTAACATCTGTTTTTCTATTATTTTGCCAAGAACAGGTAAAGTGATGGAAAATGCTTATGAACAAACTACAAGAAATGTGGTGAAGTTCTTAAATAGCTTAAATGTACCTGCTGTATTTCATGGTCGTAATGACCTTGAGATTAATAACAAGAAGTTTTCTGGGTTAGCTGAATATATCGCTAAAGACAGGTTATTAGTCCATGGAACATTATTGTTTGACACTGACTTTTCTAAGTTAGCAAAGTATTTAAATGTTGATAAGACCAAGATAGCAAGTAAGGGTGTTGACAGTGTTGCTAAGCGCGTTGTTAATGTAAAGGAGTATTTACCAAATTGA", "aa": "MLTEFRKNELVKVIYFWQNANTIVVGRNQNTYAEVNLKELESDKVNLFRRFSGGGAVFHDLGNICFSIILPRTGKVMENAYEQTTRNVVKFLNSLNVPAVFHGRNDLEINNKKFSGLAEYIAKDRLLVHGTLLFDTDFSKLAKYLNVDKTKIASKGVDSVAKRVVNVKEYLPN", "aa_hexdigest": "cdfc8bc6ac0e00bc846643ee77219928", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0037DCED81", "query_cov": 0.8901734104046243, "subject_cov": 0.8461538461538461, "identity": 0.519, "score": 162.0, "evalue": 7.59e-48, "valid": false, "product": "biotin/lipoate A/B protein ligase family protein", "uniref50_id": "UniRef50_UPI0037DCED81" }, "pscc": { "uniref50_id": "UniRef50_UPI0037DCED81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0037DCED81" ], "product": "biotin/lipoate A/B protein ligase family protein" }, "genes": [], "id": "KKNAAMEMLJ_605", "locus": "GCA000027325_00584" }, { "type": "cds", "sequence": "contig_1", "start": 328454, "stop": 329467, "strand": "-", "frame": 2, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG1249", "EC:1.8.1.4", "GO:0004148", "GO:0005737", "GO:0006090", "GO:0006103", "GO:0050660", "KEGG:K00382", "SO:0001217", "UniRef:UniRef50_P75393", "UniRef:UniRef90_P47513" ], "nt": "GTGCAAGTAAACAACACAACTTACACCACTAACAACATTATTGTTGCAACCGGATCAAGACCAAGATACTTAACTTTACCAGGGTTTGAAAAAGCACAACAAGCTGGGTTTATCATTGACTCAACCCAAGCTTTGGCTTTAGAGGGAGTACCTAAGAAGTTTGTTGTAGTTGGGGGAGGTGTGATTGGGGTTGAGTTTGCTTTTTTATTTGCTTCATTAGGGAGTGAAGTGACCATTATCCAAGGTGTTGATAGGATTTTGGAGGTTTGTGATAGTGATGTTTCTGAACTGATAAGTAAAACCTTAAAAAACAAAGGAGTTCAGATTATTACCAATGCTCATGTTGTTAGAGCTGAAAACAACCAACTGTTTTACACAGTTAATGGAGTTGAACAGTCTGTAATTGGTGATAAAATCTTAGTTTCTATAGGAAGAATTGCTAACACAGAGTGTTTAGATCAACTTGATTTAAAACGTGACCATAACAACAAAATTGTTTTAAATGAAAAACTACAAACATCAACTACAAACATCTATCTAATAGGTGATGTTAACACGCAAATGATGTTGGCACACTACGCTTACCAACAGGGCAGATATGCTGTTGATCAAATTTTGAACCAAAACCAGGTAAAGCCTGCTGAAAAAAACAAGTGTCCTGCTTGTATTTACACAAATCCTGAAGTTGCTTTTGTAGGTTATAGTGAGATGGAATTGCAAAAAGAAAAGATTGATTATGTCAAATCTTCCTTGCCATTTATTTATAGTGGTAAAGCAATTGCAGATCATGAAACCAATGGGTTTGTCAAGATGATGTTTAATCCTAAAACTGGTGCTATCTTAGGTGGATGTATTATTGCTAGCACTGCTAGTGATATTATCGCTGAGCTTGCTTTGGTGATGGAAAACAACCTCACTGTGTTTGATATTGCCAATTCTATCTCACCCCATCCTACCATGAATGAAATGGTAACTGATGTTTGTAAAAAAGCGATCTTTGATTACTTTAGTTAA", "aa": "MQVNNTTYTTNNIIVATGSRPRYLTLPGFEKAQQAGFIIDSTQALALEGVPKKFVVVGGGVIGVEFAFLFASLGSEVTIIQGVDRILEVCDSDVSELISKTLKNKGVQIITNAHVVRAENNQLFYTVNGVEQSVIGDKILVSIGRIANTECLDQLDLKRDHNNKIVLNEKLQTSTTNIYLIGDVNTQMMLAHYAYQQGRYAVDQILNQNQVKPAEKNKCPACIYTNPEVAFVGYSEMELQKEKIDYVKSSLPFIYSGKAIADHETNGFVKMMFNPKTGAILGGCIIASTASDIIAELALVMENNLTVFDIANSISPHPTMNEMVTDVCKKAIFDYFS", "aa_hexdigest": "80fec97fb6accac563db00b62595a552", "start_type": "GTG", "rbs_motif": null, "genes": [ "lpdA" ], "truncated": "3-prime", "pseudogene": { "start": 328454, "stop": 329767, "observations": { "mutation": [ 329593 ], "stop": [ 329593 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47513", "query_cov": 1.0, "subject_cov": 0.737417943107221, "identity": 0.997, "score": 655.0, "evalue": 2.01e-235, "gene_start": 1, "gene_end": 337, "reference_start": 121, "reference_end": 457, "reference_sequence": "MDYDLIILGAGPAGYIAAEYAGKHKLKTLVIEKQYFGGVCLNVGCIPTKTLLKRAKIIDYLVHAKDYGITINGQAKLDWKQLLKQKQEVVDKLVAGVKTIIKGAKVESIEGEATVIDKNKVQVNNTTYTTNNIIVATGSRPRYLTLPGFEKAQQAGFIIDSTQALALEGVPKKFVVVGGGVIGVEFAFLFASLGSEVTIIQGVDRILEVCDSDVSELISKTLKNKGVQIITNAHVVRAENNQLFYTVNGVEQSVIGDKILVSIGRIANTECLDQLDLKRDHNNKIVLNEKLQTSTTNIYLIGDVNTQMMLAHYAYQQGRYAVDQILNQNQVKPAEKNKCPACIYTNPEVAFVGYSEMELQKEKIDYVKSSLPFIYSGKAIADHETNGFVKMMFNPKTGAILGGCIIASTASDIIAELALVMENNLTVFDIANSISPHPTMNEMVTDVCKKAIFDYFS" }, "paralog": false, "identity": 0.9977116704805492, "score": 854.0, "evalue": 1.85e-316, "description": "Internal stop codon at 329593. Nonsense mutation around 329593.", "psc": { "uniref90_id": "UniRef90_P47513", "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "ec_ids": [ "1.8.1.4" ], "uniref50_id": "UniRef50_P75393", "cog_id": "COG1249", "cog_category": "C", "kegg_orthology_id": "K00382", "go_ids": [ "GO:0004148", "GO:0005737", "GO:0006090", "GO:0006103", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47513", "EC:1.8.1.4", "UniRef:UniRef50_P75393", "COG:COG1249", "COG:C", "KEGG:K00382", "GO:0004148", "GO:0005737", "GO:0006090", "GO:0006103", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75393" ], "product": "Dihydrolipoyl dehydrogenase" } }, "id": "KKNAAMEMLJ_606", "locus": "GCA000027325_00585" }, { "type": "cds", "sequence": "contig_1", "start": 329591, "stop": 329827, "strand": "-", "frame": 2, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "nt": "ATGGATTATGATCTAATTATTTTGGGTGCTGGCCCTGCTGGTTATATTGCTGCGGAGTATGCTGGCAAACATAAACTTAAAACCCTAGTGATTGAAAAGCAATACTTTGGTGGGGTGTGTTTAAATGTTGGGTGTATCCCAACTAAAACGTTGTTAAAAAGAGCAAAGATTATTGATTATTTAGTTCATGCCAAAGATTATGGTATCACTATTAATGGTCAAGCTAAACTTGATTGA", "aa": "MDYDLIILGAGPAGYIAAEYAGKHKLKTLVIEKQYFGGVCLNVGCIPTKTLLKRAKIIDYLVHAKDYGITINGQAKLD", "aa_hexdigest": "8b23540e0848f0b7478a32e15e6e2edb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0010C3BE0E", "query_cov": 0.9358974358974359, "subject_cov": 0.9358974358974359, "identity": 0.562, "score": 96.3, "evalue": 1.25e-24, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A7X1ZRV2" }, "pscc": { "uniref50_id": "UniRef50_A0A7X1ZRV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "KKNAAMEMLJ_607", "locus": "GCA000027325_00586" }, { "type": "cds", "sequence": "contig_1", "start": 329831, "stop": 330985, "strand": "-", "frame": 2, "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "db_xrefs": [ "COG:C", "COG:COG0508", "EC:2.3.1.12", "GO:0004742", "GO:0005737", "GO:0016407", "GO:0031405", "RefSeq:WP_009885904.1", "SO:0001217", "UniParc:UPI0000130BFD", "UniRef:UniRef100_P47514", "UniRef:UniRef50_P47514", "UniRef:UniRef90_P47514" ], "nt": "ATGGCAAATGAGTTTAAATTCACTGATGTTGGTGAGGGTTTACATGAAGGAAAAGTAACTGAAATCTTAAAACAAGTTGGTGATCAGATCAAGATAGATGAAGCTTTATTTGTTGTTGAAACTGATAAAGTTACAACTGAACTACCTTCTCCTTTTGCAGGTACAATTAGTGCTATTAATGTTAAAGTTGGTGATGTTGTTAGCATTGGTCAGGTGATGGCAGTTATTGGTGAAAAGACTAGTACACCACTTGTTGAACCAAAACCTCAACCAACTGAAGAAGTAGCTAAGGTAAAAGAAGCGGGGGCTTCAGTAGTAGGGGAAATTAAGGTTTCTGATAACCTCTTTCCTATCTTTGGAGTAAAACCTCATGCAACTCCAGCTGTTAAAGACACTAAAGTTGCAAGTAGTACTAACATTACTGTAGAAACAACCCAAAAACCAGAAAGTAAAACTGAACAGAAAACCATTGCTATCTCAACAATGCGTAAAGCGATTGCAGAAGCAATGACAAAGTCGCACGCAATTATCCCAACCACTGTATTAACTTTTTATGTTAATGCAACCAAGTTAAAACAATATCGTGAAAGTGTTAATGGTTATGCTTTAAGTAAGTATTCCATGAAAATTTCTTACTTTGCTTTCTTTGTTAAAGCAATTGTTAATGCGCTTAAGAAGTTCCCTGTTTTTAACGCTAGTTATGATCCTGATCAAAACGAAATTGTTTTAAATGATGACATTAATGTAGGAATTGCTGTTGATACTGAAGAAGGTTTAATTGTCCCTAACATTAAGCAAGCCCAAACCAAATCTGTGGTTGAAATTGCCCAAGCAATTGTTGATTTAGCTAACAAAGCTAGAACAAAAAAGATTAAGTTGACTGATTTGAATAAAGGTACTATTTCAGTTACTAACTTCGGTTCATTAGGAGCAGCTGTAGGTACACCTATTATTAAGTACCCTGAGATGTGTATTGTTGCTACTGGTAATTTAGAAGAACGCATTGTTAAAGTGGAAAATGGAATTGCAGTTCATACCATCTTACCTTTAACAATAGCTGCAGACCACCGCTGGGTTGATGGGGCGGATGTTGGTAGGTTTGGTAAGGAGATTGCAAAACAAATTGAGGAATTAATTGATCTTACAGTAGCTTAA", "aa": "MANEFKFTDVGEGLHEGKVTEILKQVGDQIKIDEALFVVETDKVTTELPSPFAGTISAINVKVGDVVSIGQVMAVIGEKTSTPLVEPKPQPTEEVAKVKEAGASVVGEIKVSDNLFPIFGVKPHATPAVKDTKVASSTNITVETTQKPESKTEQKTIAISTMRKAIAEAMTKSHAIIPTTVLTFYVNATKLKQYRESVNGYALSKYSMKISYFAFFVKAIVNALKKFPVFNASYDPDQNEIVLNDDINVGIAVDTEEGLIVPNIKQAQTKSVVEIAQAIVDLANKARTKKIKLTDLNKGTISVTNFGSLGAAVGTPIIKYPEMCIVATGNLEERIVKVENGIAVHTILPLTIAADHRWVDGADVGRFGKEIAKQIEELIDLTVA", "aa_hexdigest": "e84d345c1f8b149350523ebf1a1c2e25", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000130BFD", "ncbi_nrp_id": "WP_009885904.1", "uniref100_id": "UniRef100_P47514", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000130BFD", "RefSeq:WP_009885904.1", "UniRef:UniRef100_P47514" ] }, "ips": { "uniref100_id": "UniRef100_P47514", "uniref90_id": "UniRef90_P47514" }, "psc": { "uniref90_id": "UniRef90_P47514", "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "ec_ids": [ "2.3.1.12" ], "uniref50_id": "UniRef50_P47514", "cog_id": "COG0508", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0016407", "GO:0004742", "GO:0031405" ] }, "pscc": { "uniref50_id": "UniRef50_P47514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47514" ], "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex" }, "genes": [ "pdhC" ], "id": "KKNAAMEMLJ_608", "locus": "GCA000027325_00587" }, { "type": "cds", "sequence": "contig_1", "start": 331235, "stop": 331459, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02780.25" ], "nt": "GTGAAGAAAACAGGAAGACTACTTGTAGTGACTGAAGCGGTGAAAAGTTTCACTACAAGTGCAGAGATTATCACTTCAGTAACTGAAGAACTATTCACTTATCTCAAAAAAGCCCCACAACGGGTAACTGGGTTTGATATTGTTGTGCCTTTAGCTAGAGGTGAAAAATACCAGTTTGAAATTAATGCACGGGTTATTGATGCAGTTAATCAACTTTTAAAATAA", "aa": "MKKTGRLLVVTEAVKSFTTSAEIITSVTEELFTYLKKAPQRVTGFDIVVPLARGEKYQFEINARVIDAVNQLLK", "aa_hexdigest": "f5a9a38b26064ae10e52b945f1686ad8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02780.25", "name": "Transketolase_C", "length": 57, "aa_cov": 0.7702702702702703, "hmm_cov": 1.0, "evalue": 5.365168127894991e-13, "score": 44.06863021850586, "start": 1, "stop": 57 } ], "seq_stats": { "molecular_weight": 8315.6384, "isoelectric_point": 9.453788948059085 }, "id": "KKNAAMEMLJ_609", "locus": "GCA000027325_00588" }, { "type": "cds", "sequence": "contig_1", "start": 331484, "stop": 332215, "strand": "-", "frame": 2, "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "db_xrefs": [ "COG:C", "COG:COG0022", "EC:1.2.4.1", "GO:0004739", "GO:0007584", "GO:0009083", "GO:0140032", "SO:0001217", "UniRef:UniRef50_P75391", "UniRef:UniRef90_P47515" ], "nt": "ATGTCAAAAATCCAAGTAAATAACATTGAAGCGTTAAACAACGCAATGGATCTTGCACTGGAAAGAGATCAAAACGTTGTACTCTATGGCCAGGACGCTGGTTTTGAAGGGGGTGTGTTCCGTGCAACTAAAGGCTTACAACAAAAGTATGGGAGTGAAAGGGTATGGGATTGTCCTATAGCAGAAAACTCTATGGCTGGTATTGGGGTTGGGGCTGCTATAGGTGGTCTTAAACCTATTGTAGAGATCCAGTTTTCAGGCTTTTCATTCCCAGCTATGTTTCAAATCTTTGTCCATGCTGCTAGGATTAGAAACCGTTCTCGTGGTGTATATACCGCTCCACTAGTAGTGAGGATGCCAATGGGTGGGGGGATTAAAGCATTGGAACACCACAGTGAAACATTGGAAGCAATTTATGCACAGATTGCTGGGCTTAAAACAGTGATGCCATCAAATCCTTATGATACCAAAGGACTTTTTCTAGCTGCTATTGAATCACCTGATCCTGTTATCTTTTTTGAACCAAAGAAGCTTTATCGTGCTTTTCGTCAGGAGATTCCTAGTGATTATTACACTGTCCCTATTGGTGAAGCCAACTTGATTAGTGAAGGTAGTGAACTTACAATAGTTAGCTATGGTCCTACAATGTTTGATTTAATTAACTTAGTTTACAGCGGGGAATTGAAAGATAAGGGAATTGAGTTAATTGACTTGCGTACTATCTCCCCTTGA", "aa": "MSKIQVNNIEALNNAMDLALERDQNVVLYGQDAGFEGGVFRATKGLQQKYGSERVWDCPIAENSMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFVHAARIRNRSRGVYTAPLVVRMPMGGGIKALEHHSETLEAIYAQIAGLKTVMPSNPYDTKGLFLAAIESPDPVIFFEPKKLYRAFRQEIPSDYYTVPIGEANLISEGSELTIVSYGPTMFDLINLVYSGELKDKGIELIDLRTISP", "aa_hexdigest": "7647151f52bb756121fcb1af4f6e51f9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FDA3C09", "query_cov": 0.934156378600823, "subject_cov": 0.9700854700854701, "identity": 0.568, "score": 270.0, "evalue": 2.56e-88, "valid": false, "product": "transketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A537VMS1" }, "genes": [ "pdhB" ], "truncated": "5-prime", "pseudogene": { "start": 331235, "stop": 332215, "observations": { "mutation": [ 331486 ], "stop": [ 331486 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47515", "query_cov": 1.0, "subject_cov": 0.745398773006135, "identity": 1.0, "score": 480.0, "evalue": 5.35e-170, "gene_start": 1, "gene_end": 243, "reference_start": 1, "reference_end": 243, "reference_sequence": "MSKIQVNNIEALNNAMDLALERDQNVVLYGQDAGFEGGVFRATKGLQQKYGSERVWDCPIAENSMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFVHAARIRNRSRGVYTAPLVVRMPMGGGIKALEHHSETLEAIYAQIAGLKTVMPSNPYDTKGLFLAAIESPDPVIFFEPKKLYRAFRQEIPSDYYTVPIGEANLISEGSELTIVSYGPTMFDLINLVYSGELKDKGIELIDLRTISPWDKQTVFNSVKKTGRLLVVTEAVKSFTTSAEIITSVTEELFTYLKKAPQRVTGFDIVVPLARGEKYQFEINARVIDAVNQLLK" }, "paralog": false, "identity": 0.9969325153374233, "score": 635.0, "evalue": 1.08e-233, "description": "Internal stop codon at 331486. Nonsense mutation around 331486.", "psc": { "uniref90_id": "UniRef90_P47515", "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "ec_ids": [ "1.2.4.1" ], "uniref50_id": "UniRef50_P75391", "cog_id": "COG0022", "cog_category": "C", "go_ids": [ "GO:0140032", "GO:0004739", "GO:0009083", "GO:0007584" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47515", "EC:1.2.4.1", "UniRef:UniRef50_P75391", "COG:COG0022", "COG:C", "GO:0140032", "GO:0004739", "GO:0009083", "GO:0007584" ] }, "pscc": { "uniref50_id": "UniRef50_P75391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75391" ], "product": "Pyruvate dehydrogenase E1 component subunit beta" } }, "id": "KKNAAMEMLJ_610", "locus": "GCA000027325_00589" }, { "type": "cds", "sequence": "contig_1", "start": 332233, "stop": 332604, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGCAAGATGCTGCTAATTACGCTAGAGGTGGTAATGGACCAGTCTTAATTGAGTTCTTCAGCTACCGGCAAGGTCCTCACACCACTTCAGATGACCCTTCTATCTACAGAACCAAACAAGAAGAGGAGGAGGGAATGAAGAGTGATCCAGTGAAGCGGTTGCGAAACTTCTTGTTTGATAGATCAATTCTTAACCAAGCTCAAGAAGAAGAGATGTTCAGCAAAATTGAACAGGAAATCCAAGCTGCTTATGAAAAGATGGTACTAGATACTCCTGTATCAGTAGATGAGGTGTTTGATTACAACTATCAAGAATTAACCCCTGAACTAGTTGAACAGAAACAGATTGCAAAAAAATACTTTAAAGACTAA", "aa": "MQDAANYARGGNGPVLIEFFSYRQGPHTTSDDPSIYRTKQEEEEGMKSDPVKRLRNFLFDRSILNQAQEEEMFSKIEQEIQAAYEKMVLDTPVSVDEVFDYNYQELTPELVEQKQIAKKYFKD", "aa_hexdigest": "2dffca25e6e8a2e7aacb1e66941160cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 84, "aa_cov": 0.6747967479674797, "hmm_cov": 1.0, "evalue": 4.8799440063706324e-24, "score": 79.86024475097656, "start": 4, "stop": 86 } ], "seq_stats": { "molecular_weight": 14412.853499999996, "isoelectric_point": 4.650248146057129 }, "id": "KKNAAMEMLJ_611", "locus": "GCA000027325_00590" }, { "type": "cds", "sequence": "contig_1", "start": 332758, "stop": 332868, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTGCACTATAAAAAGCAACCTAATGTTGCTGTTACTATGATTGGTGATGGAGGTACAGCTGAAGGGGAATTTTATGAAGCGATGAACATTGCAAGCATCCACAAGTGA", "aa": "MLHYKKQPNVAVTMIGDGGTAEGEFYEAMNIASIHK", "aa_hexdigest": "210f36023b8fa0de2f1e35de77ddb3a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 35, "aa_cov": 0.9722222222222222, "hmm_cov": 1.0, "evalue": 2.4367500547368235e-10, "score": 34.88288497924805, "start": 2, "stop": 36 } ], "seq_stats": { "molecular_weight": 3952.4923, "isoelectric_point": 5.9887495040893555 }, "id": "KKNAAMEMLJ_612", "locus": "GCA000027325_00591" }, { "type": "cds", "sequence": "contig_1", "start": 332971, "stop": 333123, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTAAACTTCGCTCCTAATTTGGGAGAGGAAGCTTTACAGGTTGGAATGGGATTAGGTTTAAATGAAAATGATTGGGTTTGTCCTACGTTTCGTAGTGGGGCTTTAATGTTGTATCGTGGGGTAAAACCAGAACAACTTTTACTCTACTGA", "aa": "MLNFAPNLGEEALQVGMGLGLNENDWVCPTFRSGALMLYRGVKPEQLLLY", "aa_hexdigest": "ffc2ecc1afcb252a67b29e8a824f6f8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 45, "aa_cov": 0.9, "hmm_cov": 1.0, "evalue": 1.2821984037830514e-08, "score": 29.231674194335938, "start": 5, "stop": 49 } ], "seq_stats": { "molecular_weight": 5570.4433, "isoelectric_point": 4.584314918518066 }, "id": "KKNAAMEMLJ_613", "locus": "GCA000027325_00592" }, { "type": "cds", "sequence": "contig_1", "start": 333139, "stop": 333309, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATCTTGATTAAAAATAAAGTTCCAACTACCCTTTATCAGGTTTATGATAATGAGGGTAAATTAATTGATCCTAACCACAAAATTACCCTAACTGATGAACAGTTAAAACACGCTTATTACTTAATGAACTTGAGTAGAATGATGGACAAAAAGATGTTAGTTTGA", "aa": "MAILIKNKVPTTLYQVYDNEGKLIDPNHKITLTDEQLKHAYYLMNLSRMMDKKMLV", "aa_hexdigest": "397073f2a778dd24fee48785947303db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6625.819500000001, "isoelectric_point": 9.00508899688721 }, "id": "KKNAAMEMLJ_614", "locus": "GCA000027325_00593" }, { "type": "cds", "sequence": "contig_1", "start": 333337, "stop": 334419, "strand": "-", "frame": 3, "gene": "nox", "product": "NADH oxidase", "db_xrefs": [ "COG:COG0446", "COG:I", "EC:1.6.3.4", "GO:0008137", "SO:0001217", "UniRef:UniRef50_Q49408", "UniRef:UniRef90_Q49408" ], "nt": "ATGAATGTTGAAAACAAGGTGACACACAAGCCTTTGGAGTTTAACTACACTGACAAATATTGTGGTAATGTTAAGAACTTAATTAGCTGTAAGTTATACCAACATGCACTTACCTTAATCGATAGTTTTCGTAAAGATAAAACCATTAAATCAGTTGCTATTGTTGGTTCTGGTTACATTGGCTTGGAACTTGCTGAAGCAGCTTGGTTATGCAAAAAGCAAGTAACAGTAATTGACTTACTTGATAAGCCTGCTGGTAATAACTTTGATCATGAGTTTACTGATGAACTTGAAAAAGTGATGCAAAAAGATGGGTTAAAACTAATGATGGGTTGCAGTGTTAAGGGCTTTGTTGTTGATAGTACAAACAACGTTGTCAAAGGAGTTGAAACTGATAAGGGAATAGTAAATGCAGACCTTGTGATCCAATCAATTGGGTTTAGACCTAGCACTAAGTTTGTTCCTAAAGATCAAAACTTTGAGTTTATTCACAACGGTTCAATTAAAGTTAATGAATTTCTCCAAGCACTAAATCATAAGGATGTTTATGTCATTGGGGGTTGTGCTGCTATTTACAATGCTGCTAGTGAACAGTATGAAAACATCGATCTTGCTACCAATGCAGTAAAGAGTGGATTAGTAGCTGCGATGCATATCATTGGTAGTAACCAAGTTAAACTCCAATCTATCGTTGGCACCAATGCACTCCATATCTTTGGTTTAAATTTAGCAGCATGTGGATTAACTGAACAGCGTGCTAAGAAGTTAGGTTTTGATGTTGGCATATCAGTTGTTGATGACAATGATCGTCCTGAGTTTATGGGCAGTTATGACAAGGTGCGTTTTAAACTTGTATATGACAAGAAAACCCTAAGAATTTTAGGAGCACAACTCCTTTCTTGGAATACCAATCACAGTGAGATTATTTTCTATATTGCACTTGCAATCCAAAAGCAGATGTTACTAACTGAACTGGGTTTAGTGGATGTTTATTTTCTTCCACATTACAACAAACCGTTTAACTTTGTGTTAGCAACTGTTTTACAAGCACTTGGTTTTAGCTATTACATTCCTAAAAAATAG", "aa": "MNVENKVTHKPLEFNYTDKYCGNVKNLISCKLYQHALTLIDSFRKDKTIKSVAIVGSGYIGLELAEAAWLCKKQVTVIDLLDKPAGNNFDHEFTDELEKVMQKDGLKLMMGCSVKGFVVDSTNNVVKGVETDKGIVNADLVIQSIGFRPSTKFVPKDQNFEFIHNGSIKVNEFLQALNHKDVYVIGGCAAIYNAASEQYENIDLATNAVKSGLVAAMHIIGSNQVKLQSIVGTNALHIFGLNLAACGLTEQRAKKLGFDVGISVVDDNDRPEFMGSYDKVRFKLVYDKKTLRILGAQLLSWNTNHSEIIFYIALAIQKQMLLTELGLVDVYFLPHYNKPFNFVLATVLQALGFSYYIPKK", "aa_hexdigest": "1568316256934a4513c5bb8c74d72aaa", "start_type": "ATG", "rbs_motif": null, "genes": [ "nox" ], "truncated": "3-prime", "pseudogene": { "start": 333337, "stop": 334719, "observations": { "mutation": [ 334431 ], "stop": [ 334431 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49408", "query_cov": 1.0, "subject_cov": 0.7531380753138075, "identity": 1.0, "score": 715.0, "evalue": 1.35e-258, "gene_start": 1, "gene_end": 360, "reference_start": 119, "reference_end": 478, "reference_sequence": "MKKVIVIGINHAGTSFIRTLLSKSKDFKVNAYDRNTNISFLGCGIALAVSGVVKNTDDLFYSNPEELKQMGANIFMSHDVTNIDLIKKQVTVRDLTSNKEFTDQFDQLVIASGAWPICMNVENKVTHKPLEFNYTDKYCGNVKNLISCKLYQHALTLIDSFRKDKTIKSVAIVGSGYIGLELAEAAWLCKKQVTVIDLLDKPAGNNFDHEFTDELEKVMQKDGLKLMMGCSVKGFVVDSTNNVVKGVETDKGIVNADLVIQSIGFRPSTKFVPKDQNFEFIHNGSIKVNEFLQALNHKDVYVIGGCAAIYNAASEQYENIDLATNAVKSGLVAAMHIIGSNQVKLQSIVGTNALHIFGLNLAACGLTEQRAKKLGFDVGISVVDDNDRPEFMGSYDKVRFKLVYDKKTLRILGAQLLSWNTNHSEIIFYIALAIQKQMLLTELGLVDVYFLPHYNKPFNFVLATVLQALGFSYYIPKK" }, "paralog": false, "identity": 0.9978260869565218, "score": 912.0, "evalue": 0.0, "description": "Internal stop codon at 334431. Nonsense mutation around 334431.", "psc": { "uniref90_id": "UniRef90_Q49408", "gene": "nox", "product": "NADH oxidase", "ec_ids": [ "1.6.3.4" ], "uniref50_id": "UniRef50_Q49408", "cog_id": "COG0446", "cog_category": "I", "go_ids": [ "GO:0008137" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49408", "EC:1.6.3.4", "UniRef:UniRef50_Q49408", "COG:COG0446", "COG:I", "GO:0008137" ] }, "pscc": { "uniref50_id": "UniRef50_Q49408", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49408" ], "product": "NADH oxidase" } }, "id": "KKNAAMEMLJ_615", "locus": "GCA000027325_00594" }, { "type": "cds", "sequence": "contig_1", "start": 334429, "stop": 334773, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07992.20" ], "nt": "ATGAAAAAAGTGATTGTGATTGGAATAAATCACGCTGGTACTAGTTTTATTAGAACTTTACTTTCAAAAAGTAAGGACTTTAAGGTTAACGCTTATGATAGAAACACAAACATCTCGTTTCTGGGGTGTGGAATTGCACTTGCTGTTAGTGGTGTTGTTAAAAACACTGATGATCTTTTCTATTCCAACCCTGAGGAGTTGAAACAGATGGGCGCTAACATCTTTATGAGTCATGATGTTACTAACATTGATCTAATCAAAAAACAGGTAACAGTTAGAGATTTAACATCAAATAAAGAGTTCACTGATCAGTTTGATCAACTAGTAATCGCTTCAGGAGCATGA", "aa": "MKKVIVIGINHAGTSFIRTLLSKSKDFKVNAYDRNTNISFLGCGIALAVSGVVKNTDDLFYSNPEELKQMGANIFMSHDVTNIDLIKKQVTVRDLTSNKEFTDQFDQLVIASGA", "aa_hexdigest": "2993d632403822267a7101320478bd4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 115, "aa_cov": 0.9912280701754386, "hmm_cov": 0.9478260869565217, "evalue": 7.407102530149659e-18, "score": 60.00630569458008, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 12586.287599999989, "isoelectric_point": 7.853619194030762 }, "id": "KKNAAMEMLJ_616", "locus": "GCA000027325_00595" }, { "type": "cds", "sequence": "contig_1", "start": 334856, "stop": 335398, "strand": "-", "frame": 2, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_010869402.1", "SO:0001217", "UniParc:UPI0000125CDE", "UniRef:UniRef100_P47518", "UniRef:UniRef50_P75388", "UniRef:UniRef90_P47518" ], "nt": "ATGGATCAAAACTTTAAGTTGCTTGATCAAGCAATCAAGCGCTTTGAAAATTTTCCCAACCAAGGTACATTGTTTTATGACATTACCCCAGTATTTTCCAATCCCCAACTATTTAATTTTGTGCTAACCCAAATGGCACAGTTTATTAAAGCTATTAATGCAGAAGCGATAGTATGTCCTGAAGCGAGGGGTTTTATCTTTGGGGGAGCATTAGCTTCTAAAACCCAACTCCCGTTAGTATTGGTTAGAAAAGCCAATAAACTCCCAGGGCAATTAATTAGTGCTAGCTATGATTTGGAGTACAGAAAACATGCTGTATTGGAGATGTCAACCACTTCATTAATCCAAGCTAATAATGCTAAAAGGTGTGTTATTGTTGATGATGTACTTGCCACTGCTGGAACAGTTGCTGCTATTGACCAATTACTTAAACAGTTAAATGGTGAAACTGTGGGATATTGCTTTTTAATTGAGCTGAAAAAACTCAATGGTAAAGCTAAGTTACAACCAAATGTGGTTAGCAAGATTTTATTACATTACTAG", "aa": "MDQNFKLLDQAIKRFENFPNQGTLFYDITPVFSNPQLFNFVLTQMAQFIKAINAEAIVCPEARGFIFGGALASKTQLPLVLVRKANKLPGQLISASYDLEYRKHAVLEMSTTSLIQANNAKRCVIVDDVLATAGTVAAIDQLLKQLNGETVGYCFLIELKKLNGKAKLQPNVVSKILLHY", "aa_hexdigest": "2101c24b54a703e57741de4f79e240c8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125CDE", "ncbi_nrp_id": "WP_010869402.1", "uniref100_id": "UniRef100_P47518", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125CDE", "RefSeq:WP_010869402.1", "UniRef:UniRef100_P47518" ] }, "ips": { "uniref100_id": "UniRef100_P47518", "uniref90_id": "UniRef90_P47518" }, "psc": { "uniref90_id": "UniRef90_P47518", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_P75388", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_P75388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75388" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "KKNAAMEMLJ_617", "locus": "GCA000027325_00596" }, { "type": "cds", "sequence": "contig_1", "start": 335526, "stop": 336212, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACACAGACTTTTTTATAACCAAAAACCAACATGATTTCTTTTTGAAAAACAACTGGAAGTTTTTGTTTGTATGGCTAATCTTGTTAGCTATAGGAGTTGTAATGCTAGTTTTATATCTTGTTCAACCTAACCTGTTAGGAGAATTTTTAGCAGCAGATGTTGAATCAAGTAACGGGATTATTGCTGGGATAGGAATAGTTAGTTTACTGTACTTAGCTTATTGTTTGATTAGATATGGTGTTGCTTATTGTTTAAGTTATTTGGTTAGCTTTATCTTGTTATGTAGTGGTTTGTTTGCAGTGATGTATCTAACTAACTATCTGTTTAGTATAGACCAATCAACAATCCAGTTAATTACCTTTGTTTATCTGTTTTGGCTCTTTTTTGCAGCTAAGGTATCACAAACAACAACTTGGACTTACTTTTACTGGTTTAAAAGGAGCTTAAAGGACAAGGTATTTATCAAAAACTTATTTAACGATAACTTCAACTCGCAGTGGAAGATAGATCTGATTGAAAGTTCTAGCTTAGTTTTCATTTTTATTATCTACAGTGGTTTTAACTTTGGTGGGATTGATGGCAACTTTAACCTTGTGATCTTTTATCTAATTGCTATTGTTGGTTTATTTGATGTTGCAACTGCTTTTTTACCAATGTTTTGCTTTGGTTTAATCAATGGTTGA", "aa": "MNTDFFITKNQHDFFLKNNWKFLFVWLILLAIGVVMLVLYLVQPNLLGEFLAADVESSNGIIAGIGIVSLLYLAYCLIRYGVAYCLSYLVSFILLCSGLFAVMYLTNYLFSIDQSTIQLITFVYLFWLFFAAKVSQTTTWTYFYWFKRSLKDKVFIKNLFNDNFNSQWKIDLIESSSLVFIFIIYSGFNFGGIDGNFNLVIFYLIAIVGLFDVATAFLPMFCFGLING", "aa_hexdigest": "9a4891a76217b07c8c1a4a4160d0d815", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26266.718400000016, "isoelectric_point": 5.678522300720215 }, "id": "KKNAAMEMLJ_618", "locus": "GCA000027325_00597" }, { "type": "cds", "sequence": "contig_1", "start": 336507, "stop": 336941, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTAGTGATGGTACATACACCCCACCAAACTTTGTAACAGCAGCTACTGTTAAAGAGCTTTTAACAAATCCATTTCCCACAGCAGCTACCATTGCAACTGCAAAAACAAGTCTGGTTAACGCTCCACTTGCAAATACCATCACAGATGTTTCTAATTTTGCTTCTAGTTTTATTGCGTTTGGTATCATCGTTCTTATTGCTGCTGTTCTATTAACACTACGATACAAGCTCTTAGGGTTATACAAAGCATTAGCTTTAGGGTTAAGTGTGGTGTCATCACTGGTTATCTTTAGTGCAGTTGGTGGAGTTGTTGATGTCTTTTCTTTTGTGGGCATCTTCTTTGTTATTGCCATCAACCTAATTAATCTCTTGAATTTAGGAGAGTTATTCTTAAGAAATATTAGAAATAATGCTTCTATTATCGAATCGTGA", "aa": "MSSDGTYTPPNFVTAATVKELLTNPFPTAATIATAKTSLVNAPLANTITDVSNFASSFIAFGIIVLIAAVLLTLRYKLLGLYKALALGLSVVSSLVIFSAVGGVVDVFSFVGIFFVIAINLINLLNLGELFLRNIRNNASIIES", "aa_hexdigest": "ff495a4c71ad10b7750c1290b81a5648", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15169.665199999989, "isoelectric_point": 8.06391506195068 }, "id": "KKNAAMEMLJ_619", "locus": "GCA000027325_00598" }, { "type": "cds", "sequence": "contig_1", "start": 337485, "stop": 337940, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAATGGGGGATTACCTTCTGTTACTCTCACCTTTGAACAAAACCGTGAGCAAGCCAAAACCCGCCAAAGAAAAAGACAAGTTGATGCTCAGGTTAGAAGAAATGCAATTAGTTCAGTTGAACACAACTACCAACTTTCACTTGAAACAACTGATGGAGTGGTTTTATTTGATTCACTGGATAATAATTTTATTAATGCTTCCATTAGGGCAGTTGTTCCCCAAAACACTTCTGTAAACAGTGCTTTAACCTTTGAATACAAGTTAAATAAAAATGTAGTTACCAAAGAAAGTTTACACACCGATTTTCTTGATTTTATCAACTCTAAAAACTTATCAAGTTCTGATTATAATACTGGTAATGGGCAAGCTAGTGTTGAGGGCAATGGTAAATTTTTTAAGCAAAATGCGAATGGAACTTCAAGTAGTGGTAATAAAACTTTAGTATTGTGA", "aa": "MVNGGLPSVTLTFEQNREQAKTRQRKRQVDAQVRRNAISSVEHNYQLSLETTDGVVLFDSLDNNFINASIRAVVPQNTSVNSALTFEYKLNKNVVTKESLHTDFLDFINSKNLSSSDYNTGNGQASVEGNGKFFKQNANGTSSSGNKTLVL", "aa_hexdigest": "b97b2bf3ac8d845a7bbce7e6cb5459ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16649.161899999985, "isoelectric_point": 8.961830711364748 }, "id": "KKNAAMEMLJ_620", "locus": "GCA000027325_00599" }, { "type": "cds", "sequence": "contig_1", "start": 337941, "stop": 338249, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTGTTATTTTTGGGAGTTACAAACTGTTAAATGACAGCAGGTTAGGTGCTGTTTTCAACGGTTCTACCACTACAACTGTTTACTTTTTAAACCATAAGTCAACTAACAATACCTCATTAGATCCTCAGCAAACAAAACCAACCAACGGGATTGAAAACATCACTAACATTGATAGCTTTTTAGATGGTGTTGAAAAAAGTTATGCTAACAGTTTATTCATTCAGGGCTTTTCTTCTGTAAACATTACTAAAAACACTAATGATAAAACTGCTAGTGAACTTGACAACATTGACAAGTCCTGA", "aa": "MFGVIFGSYKLLNDSRLGAVFNGSTTTTVYFLNHKSTNNTSLDPQQTKPTNGIENITNIDSFLDGVEKSYANSLFIQGFSSVNITKNTNDKTASELDNIDKS", "aa_hexdigest": "227633aa5314c59c3396f6de1da7e28e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11178.170399999988, "isoelectric_point": 5.107802009582519 }, "id": "KKNAAMEMLJ_621", "locus": "GCA000027325_00600" }, { "type": "cds", "sequence": "contig_1", "start": 338367, "stop": 339755, "strand": "+", "frame": 3, "gene": "spoT", "product": "putative guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "db_xrefs": [ "COG:COG0317", "COG:TK", "EC:3.1.7.2", "GO:0008893", "GO:0015970", "SO:0001217", "UniRef:UniRef50_P75386", "UniRef:UniRef90_P47520" ], "nt": "ATGGCAACCATTCAGGAAATCGAGTGTGATTTTTTAGCTAAAATAGCACAAAAATTTACTAATGCAGAGATTGAATTAATTAACAAAGCATTCTATCACGCTAAAACTTGGCATGAAAACCAGAAACGGCTTAGCGGTGAACCTTTTTTTATCCATCCTTTAAGAACGGCATTATCACTAGTTGAATGGAACATGGATCCTATCACTATTTGTGCTGGTTTGTTACATGACATCATTGAAGATACAGACCAAACCGAAGCTAATATAGCAATGATTTTTAGCAAAGAAATTGCTGAGCTTGTCACTAAGGTTACAAAGATTACCAATGAATCTAAAAAGCAACGTCATCTCAAAAATAAAAAGGAGAATCTTAACTTAAAAAGCTTTGTTAACATTGCAATCAATTCTCAACAAGAGATAAATGTAATGGTACTAAAACTAGCAGATCGACTTGATAACATCGCTTCCATTGAGTTTCTCCCCATTGAAAAGCAAAAGGTAATTGCAAAAGAAACTTTAGAACTTTATGCAAAGATTGCTGGGAGGATTGGGATGTATCCTGTTAAAACAAAATTAGCAGATCTTTCATTTAAGGTGTTGGATTTAAAAAACTATGATAACACCCTGTCAAAGATTAACAAGCAAAAGGTCTTTTATGACAATGAGTGGGATAACTTCAAACAACAATTAAAAAAAATCTTAGCGCAAAATCAGATAGAATACCAACTTGAAAGTCGGATTAAAGGCATTTACTCTACATATAAAAAACTAACTGTTCATGAACAGAACATCAGTAAGATCCATGATCTTTTTGCTATCCGCTTAATTACTAAATCAGAACTTGATTGTTATCACATCCTTGGTTTAATTCACCTTAATTTTTTAATTGACAGTAAATACTTCAAAGACTATATTGCCTCACCTAAACAAAACCTTTACCAATCAATTCATACCACTGTTCGTTTAAAAGGGTTAAATGTTGAGATCCAAATTAGAACCCAACAGATGGACAATGTTAGTAAGTTTGGCTTAGCTAGTCACTGGATCTACAAAGAACAGAAAGAGGGATTGTTAGCACCTGCTTTGCAACTTAATTACCTAGTGACAAAACAAAAACACTCACATGATTTTCTAAAAAGGATTTTTGGGACTGATATTATCAAGATTAATGTTAGTGCTAGTCATGAACCTAATGTAATTAAGCAAATTAATGTTGATAGCAACAATAAACTCCTTGATATTGCTTTTGAAAACTATCCCAAGCAATTTGCTAAATTAACCAAAATTGAAATTGATGGGGTTGAGATCAATTCTTTTGATACTAGTGTTGAAAATGAGATGCTGATTGAATTTTACTTTGGCAAGAATAACAATTTGAAATCAAAGTGA", "aa": "MATIQEIECDFLAKIAQKFTNAEIELINKAFYHAKTWHENQKRLSGEPFFIHPLRTALSLVEWNMDPITICAGLLHDIIEDTDQTEANIAMIFSKEIAELVTKVTKITNESKKQRHLKNKKENLNLKSFVNIAINSQQEINVMVLKLADRLDNIASIEFLPIEKQKVIAKETLELYAKIAGRIGMYPVKTKLADLSFKVLDLKNYDNTLSKINKQKVFYDNEWDNFKQQLKKILAQNQIEYQLESRIKGIYSTYKKLTVHEQNISKIHDLFAIRLITKSELDCYHILGLIHLNFLIDSKYFKDYIASPKQNLYQSIHTTVRLKGLNVEIQIRTQQMDNVSKFGLASHWIYKEQKEGLLAPALQLNYLVTKQKHSHDFLKRIFGTDIIKINVSASHEPNVIKQINVDSNNKLLDIAFENYPKQFAKLTKIEIDGVEINSFDTSVENEMLIEFYFGKNNNLKSK", "aa_hexdigest": "c2ce9f4107d3fe425fb9c628829ad232", "start_type": "ATG", "rbs_motif": null, "genes": [ "spoT" ], "truncated": "3-prime", "pseudogene": { "start": 338367, "stop": 340058, "observations": { "mutation": [ 339753, 340020 ], "stop": [ 339753, 340020 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47520", "query_cov": 1.0, "subject_cov": 0.6416666666666667, "identity": 1.0, "score": 895.0, "evalue": 0.0, "gene_start": 1, "gene_end": 462, "reference_start": 1, "reference_end": 462, "reference_sequence": "MATIQEIECDFLAKIAQKFTNAEIELINKAFYHAKTWHENQKRLSGEPFFIHPLRTALSLVEWNMDPITICAGLLHDIIEDTDQTEANIAMIFSKEIAELVTKVTKITNESKKQRHLKNKKENLNLKSFVNIAINSQQEINVMVLKLADRLDNIASIEFLPIEKQKVIAKETLELYAKIAGRIGMYPVKTKLADLSFKVLDLKNYDNTLSKINKQKVFYDNEWDNFKQQLKKILAQNQIEYQLESRIKGIYSTYKKLTVHEQNISKIHDLFAIRLITKSELDCYHILGLIHLNFLIDSKYFKDYIASPKQNLYQSIHTTVRLKGLNVEIQIRTQQMDNVSKFGLASHWIYKEQKEGLLAPALQLNYLVTKQKHSHDFLKRIFGTDIIKINVSASHEPNVIKQINVDSNNKLLDIAFENYPKQFAKLTKIEIDGVEINSFDTSVENEMLIEFYFGKNNNLKSKWIRYMNNPIYREKVKKSLAKLAKSGRYSELAFYEKELGEKQLKLASETEIQKRLNTLRIKKMSDYLALIECTNFTNDEHLLFLAKNNDKWNKLTKPLKFAFSKVVFHNSYFEQIEGIFITKIVIEPCCSKIPDMPEQVTGILTKNILSVHRYGCKNLQNKKQLKIIPLYWNIQQLKLKPRKFRSYININGVWSEKTINKICQTIINGDGYIEKIIPKINKQKDEFDLNITLFVNNYQQLLTLMDQITTKNISFSWKYL" }, "paralog": false, "identity": 0.9964476021314387, "score": 1103.0, "evalue": 0.0, "description": "Internal stop codon at 339753, 340020. Nonsense mutation around 339753, 340020.", "psc": { "uniref90_id": "UniRef90_P47520", "gene": "spoT", "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "ec_ids": [ "3.1.7.2" ], "uniref50_id": "UniRef50_P75386", "cog_id": "COG0317", "cog_category": "TK", "go_ids": [ "GO:0008893", "GO:0015970" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47520", "EC:3.1.7.2", "UniRef:UniRef50_P75386", "COG:COG0317", "COG:TK", "GO:0008893", "GO:0015970" ] }, "pscc": { "uniref50_id": "UniRef50_P75386", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75386" ], "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase" } }, "id": "KKNAAMEMLJ_622", "locus": "GCA000027325_00601" }, { "type": "cds", "sequence": "contig_1", "start": 339765, "stop": 340022, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAACCCTATATACCGTGAAAAGGTAAAAAAGAGCTTGGCTAAACTAGCTAAATCTGGTAGATACAGTGAGTTAGCTTTTTATGAAAAAGAACTGGGTGAAAAACAGTTAAAACTTGCTAGTGAAACTGAAATCCAAAAACGCTTAAACACCCTAAGAATTAAAAAAATGAGTGATTACTTAGCGTTAATTGAGTGTACTAACTTTACTAATGATGAACATTTGTTGTTTCTAGCTAAAAACAACGACAAGTGA", "aa": "MNNPIYREKVKKSLAKLAKSGRYSELAFYEKELGEKQLKLASETEIQKRLNTLRIKKMSDYLALIECTNFTNDEHLLFLAKNNDK", "aa_hexdigest": "83f0efcfaca7b795c6eb7c044778a58d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9968.448699999986, "isoelectric_point": 9.380037117004395 }, "id": "KKNAAMEMLJ_623", "locus": "GCA000027325_00602" }, { "type": "cds", "sequence": "contig_1", "start": 340119, "stop": 340262, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGAACCATGTTGTAGTAAGATCCCTGATATGCCTGAACAAGTAACTGGTATCTTAACTAAAAACATTTTAAGTGTTCACCGTTATGGTTGTAAGAATTTACAAAATAAAAAGCAGTTAAAAATTATCCCGTTATATTGA", "aa": "MIEPCCSKIPDMPEQVTGILTKNILSVHRYGCKNLQNKKQLKIIPLY", "aa_hexdigest": "705a2ebcf5d94b3fa38f79fd7e531505", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5400.473400000001, "isoelectric_point": 9.21854381561279 }, "id": "KKNAAMEMLJ_624", "locus": "GCA000027325_00603" }, { "type": "cds", "sequence": "contig_1", "start": 340323, "stop": 340517, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGGAGTGAAAAAACCATTAATAAAATCTGTCAAACAATTATTAATGGTGATGGTTATATTGAAAAAATAATTCCCAAGATCAACAAACAAAAAGATGAATTTGATTTAAACATCACCCTTTTTGTTAATAACTACCAACAACTTCTCACCTTAATGGACCAAATTACCACTAAGAATATCAGCTTTAGTTGA", "aa": "MWSEKTINKICQTIINGDGYIEKIIPKINKQKDEFDLNITLFVNNYQQLLTLMDQITTKNISFS", "aa_hexdigest": "6eee32e0da4f977d666e149445b966b7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7510.615500000003, "isoelectric_point": 6.07798671722412 }, "id": "KKNAAMEMLJ_625", "locus": "GCA000027325_00604" }, { "type": "cds", "sequence": "contig_1", "start": 340526, "stop": 341182, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG279", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010869404.1", "SO:0001217", "UniParc:UPI000013956C", "UniRef:UniRef100_P47521", "UniRef:UniRef50_P47521", "UniRef:UniRef90_P47521" ], "nt": "ATGATTAGACTATTAAAAAAACTTGCAGTTTTTCTTATTATTTTGGTAGGGATTTTATTGTTAGGTGGAATTGCTACTGCTGGTTATTTTGCTTTTACTTACCGTGAACCTATTAACAACTACTATAAAGAGGGATATAACAAGATTAGTGAATATAACACTGAAATTAAAAAGATCTCCCAAAATATCTTCCAAAATAACCTAGTTAAAACACTTTCAGAAGTTGAAAAATCTTTAAACGAAGGGCGAAAATTAACGCAAAACAATTCATTTGCATCAGGATTAGATAGTTCTTTAAATGCACTTGAAGGTTCATTGAAAAAAATTAATAACTTTGATAGTAATGCTGCTTTTACCCAAATTAAACACACATTAAATAACATAACTAGCTTTGTTGATCAAATGCTAGAAAAGTTCCCAAATCCAAACCAAAATGATGATTTTAAGCGGTATTTAACTGAAGTTTCCCAAATCCTTTTCTATACAGGAATCTCAATTATTGGGGCATTTTTTGTTTCTGGTTTTTTATTAATTTTATTTACAAAGAAAGTTTATGGAGTGAGAGTATCACGTTTCAATCCACAACGGCTACTAAAAAAGCACTTAGTTTTACTTTTAAGAGATGAAGAAGTGTATGATGCGGTGTTTGGTAACTAA", "aa": "MIRLLKKLAVFLIILVGILLLGGIATAGYFAFTYREPINNYYKEGYNKISEYNTEIKKISQNIFQNNLVKTLSEVEKSLNEGRKLTQNNSFASGLDSSLNALEGSLKKINNFDSNAAFTQIKHTLNNITSFVDQMLEKFPNPNQNDDFKRYLTEVSQILFYTGISIIGAFFVSGFLLILFTKKVYGVRVSRFNPQRLLKKHLVLLLRDEEVYDAVFGN", "aa_hexdigest": "8a0de76af8c9d3549e1a418cf8cbf872", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013956C", "ncbi_nrp_id": "WP_010869404.1", "uniref100_id": "UniRef100_P47521", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013956C", "RefSeq:WP_010869404.1", "UniRef:UniRef100_P47521" ] }, "ips": { "uniref100_id": "UniRef100_P47521", "uniref90_id": "UniRef90_P47521" }, "psc": { "uniref90_id": "UniRef90_P47521", "product": "Uncharacterized protein MG279", "uniref50_id": "UniRef50_P47521", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47521", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47521" ], "product": "Uncharacterized protein MG279" }, "genes": [], "id": "KKNAAMEMLJ_626", "locus": "GCA000027325_00605" }, { "type": "cds", "sequence": "contig_1", "start": 341175, "stop": 341972, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG280", "db_xrefs": [ "GO:0005886", "RefSeq:WP_009885910.1", "SO:0001217", "UniParc:UPI0000139574", "UniRef:UniRef100_P47522", "UniRef:UniRef50_P47522", "UniRef:UniRef90_P47522" ], "nt": "ATGGTTATTTTTATTAATCGGTTTTCGAAAACCATCTTGCTTTTCTTTGGAATGTTGGTTTTTGTATTTCTGTTAGGGTTTGGTATTACTGCTTTGTATTTTCGTAGTACAGCAGCTAACCTTTATGTTCAAGCTCGTAATTCCATTGATAGTTCTTTCAATTCTGCTAAAGCCTTTGCTAATGCTTTAGCAAATAGTGCTAACCAATTTAGCAAAAGTTCCATTACTAACAATTTAGATCAAGTTAAAAAAGACTTAGAGCAATCTTTACAAAAAGTGGATGAATACAAAAAGAACTTAGAGAGTCAGAACAATCTAGGTAATATTAGTCAAGAAAAGATTAGGGAACTTGATGCAACCAAAAAAGACTTGGAAAACAGTAAAACACAATTAGACAACTTTAAAAATAATCTTGATAAAAATGGTACTGCTAGTTCATCTCCATCTGTAAAAAAACAAGCAACAGCTGATGGTGTAATTAGTGCAGTTAGTGAGTTTTCTACACAAGCACAAAGCATTGTTAGTAGCTATGAAAAGATCAAAAACAACATCCCTTCTTCTGAACAGTTCAATAATTATTATGATGTCACAATGATAACCATTGTTGCTGTATCAGGTGGGATGTTAGCAATTTTGATTACAACCATTGTCTTTTCCTTTTTAACTTCTAAAAAAAGAGGATTGATCAGGTTTTCAAGATTTATTAGTACTGAACAGTTAGCTGATCATGTTAATGATATCTTAGATCGTTATCCTGATTTGGAAGAGGAAGTGATAACAGCATTAGACCATGATTAG", "aa": "MVIFINRFSKTILLFFGMLVFVFLLGFGITALYFRSTAANLYVQARNSIDSSFNSAKAFANALANSANQFSKSSITNNLDQVKKDLEQSLQKVDEYKKNLESQNNLGNISQEKIRELDATKKDLENSKTQLDNFKNNLDKNGTASSSPSVKKQATADGVISAVSEFSTQAQSIVSSYEKIKNNIPSSEQFNNYYDVTMITIVAVSGGMLAILITTIVFSFLTSKKRGLIRFSRFISTEQLADHVNDILDRYPDLEEEVITALDHD", "aa_hexdigest": "fadd889026510e58c74f3bfdbfb95dd6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139574", "ncbi_nrp_id": "WP_009885910.1", "uniref100_id": "UniRef100_P47522", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139574", "RefSeq:WP_009885910.1", "UniRef:UniRef100_P47522" ] }, "ips": { "uniref100_id": "UniRef100_P47522", "uniref90_id": "UniRef90_P47522" }, "psc": { "uniref90_id": "UniRef90_P47522", "product": "Uncharacterized protein MG280", "uniref50_id": "UniRef50_P47522", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47522", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47522" ], "product": "Uncharacterized protein MG280" }, "genes": [], "id": "KKNAAMEMLJ_627", "locus": "GCA000027325_00606" }, { "type": "cds", "sequence": "contig_1", "start": 342033, "stop": 342863, "strand": "-", "frame": 1, "gene": null, "product": "IgG-blocking protein M", "db_xrefs": [ "GO:0005515", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47523", "UniRef:UniRef90_P47523" ], "nt": "TTGCAATTACCAGAATCAGTTAAAAAAGTTTCACTTTATGGTGATTACACTGGTGTTAATGTAGCTAAACAAATCTTTGCAAACGTAGTTGAGTTGGAATTTTATTCCACCAGTAAAGCAAATAGTTTTGGTTTTAACCCATTAGTATTGGGAAGTAAAACTAATGTTATCTATGATCTGTTTGCCTCAAAACCATTTACCCACATTGATTTAACCCAAGTTACACTCCAAAACAGTGATAATTCAGCCATTGATGCTAATAAGCTCAAACAAGCAGTTGGTGATATTTACAACTACAGAAGGTTTGAAAGACAGTTCCAAGGTTATTTTGCTGGGGGATACATTGACAAGTACTTAGTTAAGAATGTTAATACCAACAAGGATAGTGATGATGATTTGGTGTACCGTTCTTTAAAAGAACTTAACCTCCACTTAGAAGAAGCTTACAGAGAAGGAGATAACACCTATTACAGGGTTAATGAAAACTATTACCCTGGTGCTAGCATCTATGAAAATGAACGCGCAAGCAGAGACAGCGAGTTTCAAAATGAAATCTTAAAACGTGCTGAACAAAACGGAGTAACATTTGATGAAAACATCAAAAGAATTACTGCTAGTGGTAAATACAGTGTACAGTTCCAAAAACTAGAAAATGATACAGATAGTAGTCTTGAAAGAATGACTAAAGCAGTTGAAGGGTTGGTTACTGTAATTGGTGAAGAAAAGTTTGAAACAGTTGATATTACTGGTGTTTCCAGTGATACAAATGAAGTTAAATCACTAGCAAAAGAACTTAAAACCAACGCATTGGGTGTTAAACTTAAACTCTAG", "aa": "MQLPESVKKVSLYGDYTGVNVAKQIFANVVELEFYSTSKANSFGFNPLVLGSKTNVIYDLFASKPFTHIDLTQVTLQNSDNSAIDANKLKQAVGDIYNYRRFERQFQGYFAGGYIDKYLVKNVNTNKDSDDDLVYRSLKELNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEFQNEILKRAEQNGVTFDENIKRITASGKYSVQFQKLENDTDSSLERMTKAVEGLVTVIGEEKFETVDITGVSSDTNEVKSLAKELKTNALGVKLKL", "aa_hexdigest": "2832ab3b78cfd7c2e74be53a2d48d8a9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 342033, "stop": 343163, "observations": { "mutation": [ 343049 ], "stop": [ 343049 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47523", "query_cov": 1.0, "subject_cov": 0.49640287769784175, "identity": 0.996, "score": 535.0, "evalue": 1.6e-187, "gene_start": 1, "gene_end": 276, "reference_start": 281, "reference_end": 556, "reference_sequence": "MQFKKHKNSVKFKRKLFWTIGVLGAGALTTFSAVMITNLVNQSGYALVASGRSGNLGFKLFSTQSPSAEVKLKSLSLNDGSYQSEIDLSGGANFREKFRNFANELSEAITNSPKGLDRPVPKTEISGLIKTGDNFITPSFKAGYYDHVASDGSLLSYYQSTEYFNNRVLMPILQTTNGTLMANNRGYDDVFRQVPSFSGWSNTKATTVSTSNNLTYDKWTYFAAKGSPLYDSYPNHFFEDVKTLAIDAKDISALKTTIDSEKPTYLIIRGLSGNGSQLNELQLPESVKKVSLYGDYTGVNVAKQIFANVVELEFYSTSKANSFGFNPLVLGSKTNVIYDLFASKPFTHIDLTQVTLQNSDNSAIDANKLKQAVGDIYNYRRFERQFQGYFAGGYIDKYLVKNVNTNKDSDDDLVYRSLKELNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEFQNEILKRAEQNGVTFDENIKRITASGKYSVQFQKLENDTDSSLERMTKAVEGLVTVIGEEKFETVDITGVSSDTNEVKSLAKELKTNALGVKLKL" }, "paralog": false, "identity": 0.9973404255319149, "score": 741.0, "evalue": 1.89e-271, "description": "Internal stop codon at 343049. Nonsense mutation around 343049.", "psc": { "uniref90_id": "UniRef90_P47523", "product": "IgG-blocking protein M", "uniref50_id": "UniRef50_P47523", "go_ids": [ "GO:0005515", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47523", "UniRef:UniRef50_P47523", "GO:0005515", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47523" ], "product": "Uncharacterized protein MG281" } }, "id": "KKNAAMEMLJ_628", "locus": "GCA000027325_00607" }, { "type": "cds", "sequence": "contig_1", "start": 343047, "stop": 343703, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20757.2" ], "nt": "ATGCAATTTAAAAAACATAAAAATAGTGTGAAATTTAAGCGTAAATTATTTTGGACTATTGGTGTTTTAGGTGCTGGAGCGTTAACCACATTCTCAGCAGTTATGATCACTAACCTAGTTAATCAAAGTGGTTATGCTTTGGTTGCATCAGGTAGGAGTGGTAACCTCGGTTTTAAGTTGTTCTCAACACAATCTCCTAGTGCAGAAGTAAAGTTGAAATCACTTTCTTTAAACGATGGTAGCTATCAGAGTGAGATTGATTTATCTGGTGGTGCTAACTTCCGTGAGAAATTTAGAAACTTTGCTAATGAGTTATCAGAAGCGATCACTAACAGTCCAAAGGGTTTAGACCGTCCTGTTCCTAAAACTGAAATTAGTGGCTTAATTAAAACAGGTGATAACTTTATTACTCCTAGTTTTAAAGCTGGTTATTATGATCATGTTGCTAGTGATGGTTCACTGCTTAGCTATTACCAATCAACTGAATATTTCAATAACCGCGTGTTGATGCCAATCTTGCAAACAACTAACGGTACTCTCATGGCAAACAACCGTGGTTATGATGATGTGTTTAGACAAGTTCCTAGTTTCAGCGGATGGAGTAACACAAAAGCAACAACTGTTTCAACTAGTAATAACCTTACTTATGATAAGTGA", "aa": "MQFKKHKNSVKFKRKLFWTIGVLGAGALTTFSAVMITNLVNQSGYALVASGRSGNLGFKLFSTQSPSAEVKLKSLSLNDGSYQSEIDLSGGANFREKFRNFANELSEAITNSPKGLDRPVPKTEISGLIKTGDNFITPSFKAGYYDHVASDGSLLSYYQSTEYFNNRVLMPILQTTNGTLMANNRGYDDVFRQVPSFSGWSNTKATTVSTSNNLTYDK", "aa_hexdigest": "c52903da0bae22e9a6861f62020bc02e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20757.2", "name": "M_large_dom", "length": 147, "aa_cov": 0.6422018348623854, "hmm_cov": 1.0, "evalue": 3.2291866540498e-85, "score": 281.374755859375, "start": 79, "stop": 218 } ], "seq_stats": { "molecular_weight": 23981.675199999998, "isoelectric_point": 9.587431907653805 }, "id": "KKNAAMEMLJ_629", "locus": "GCA000027325_00608" }, { "type": "tRNA", "sequence": "contig_1", "start": 343884, "stop": 343957, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 73.6, "nt": "GCGAGTATAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGATCGTGTCGGTTCGATTCCGATTACTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 343923, 343925 ], "id": "KKNAAMEMLJ_30", "locus": "GCA000027325_00609" }, { "type": "tRNA", "sequence": "contig_1", "start": 343965, "stop": 344050, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 72.3, "nt": "GCCCAAGTGGCGGAATGGTAGACGCATGGGATTTAAGATCCCACGCCAGTAATGGTGTGCCGGTTCAAGTCCGGCTTTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 344015, 344017 ], "id": "KKNAAMEMLJ_29", "locus": "GCA000027325_00610" }, { "type": "tRNA", "sequence": "contig_1", "start": 344051, "stop": 344125, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 74.0, "nt": "GACTCACTAGCTCAGCGGTAGAGCATTTGACTTTTAATCAAAGGGTCCCGAGTTCGATCCTCGGGTGGGTCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 344098, 344100 ], "id": "KKNAAMEMLJ_28", "locus": "GCA000027325_00611" }, { "type": "tRNA", "sequence": "contig_1", "start": 344172, "stop": 344246, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 70.6, "nt": "TGGGATGTAGCCAAGCGGTAAGGCAATAGACTTTGACTCTATCATGCGATGGTTCGATCCCATCCATCCCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 344212, 344214 ], "id": "KKNAAMEMLJ_27", "locus": "GCA000027325_00612" }, { "type": "tRNA", "sequence": "contig_1", "start": 344251, "stop": 344337, "strand": "-", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 72.6, "nt": "GGACAGGTAGCGAAGTGGCTAAACGCTTCTGACTGTAGATCAGACACCTTTATGGTTTCGGGAGTTCGAATCTCTCCCTGTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 344329, 344331 ], "id": "KKNAAMEMLJ_26", "locus": "GCA000027325_00613" }, { "type": "cds", "sequence": "contig_1", "start": 344365, "stop": 344850, "strand": "-", "frame": 3, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063", "RefSeq:WP_009885993.1", "SO:0001217", "UniParc:UPI000012BAA4", "UniRef:UniRef100_P47524", "UniRef:UniRef50_P47524", "UniRef:UniRef90_P47524" ], "nt": "ATGGAACTAAATAAAAATTACCTAACTCAAGAAGGATTTAAGCAACTGGAAAAAGAACTTGAAAACCTAATCCAAGTTAAACGTCCTGAGATTATCAGACTCTTACAAGAAGCACGTGATCAGGGTGATTTAAGTGAAAATGCTGATTATGATGCAGCTAAAGCACAGCAAGGTGAGATTGAAACTAGGATTGCTGAAATTCAAGATATATTAGCCAACGCTAAGTTAATTAGTGACCACCAAGCAAAAACCAAAGTAACAAAAGTTAGCTTAGGGAGCACTGTTGAGATCTATGATTACAGTTCTAAATCGAACGAAAAATACACAATTGTAGGTACACTTGAAGCAAATCCTGAAGAACACAAAATTTCCAATGAATCACCCCTTGCCCTTGCAATCTATGGGCGTTTAATTGGTGATGAATGTGATGTTGTTGGTATTGAAGTTCCCTATCGTGTTAAAATCCTGAAGATCAGCAACAGATAA", "aa": "MELNKNYLTQEGFKQLEKELENLIQVKRPEIIRLLQEARDQGDLSENADYDAAKAQQGEIETRIAEIQDILANAKLISDHQAKTKVTKVSLGSTVEIYDYSSKSNEKYTIVGTLEANPEEHKISNESPLALAIYGRLIGDECDVVGIEVPYRVKILKISNR", "aa_hexdigest": "d2968165f7cac4163cfd0cda1e5c4990", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012BAA4", "ncbi_nrp_id": "WP_009885993.1", "uniref100_id": "UniRef100_P47524", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012BAA4", "RefSeq:WP_009885993.1", "UniRef:UniRef100_P47524" ] }, "ips": { "uniref100_id": "UniRef100_P47524", "uniref90_id": "UniRef90_P47524" }, "psc": { "uniref90_id": "UniRef90_P47524", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_P47524", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_P47524", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47524" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "KKNAAMEMLJ_630", "locus": "GCA000027325_00614" }, { "type": "cds", "sequence": "contig_1", "start": 345031, "stop": 345165, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W775" ], "nt": "ATGAGAGAAAACAATAGTAATGCTCAAAACAAAAAGAAGATCCGCTTTTTAGCTTTGGGCATCATTATTCTAATCGTACTAATTGCTTTAATTACTATGCTTTTTATCACTGGAGTAATTAGTCGCAACCGTTAA", "aa": "MRENNSNAQNKKKIRFLALGIIILIVLIALITMLFITGVISRNR", "aa_hexdigest": "37053122028846411abf77e2ea861a2f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W775", "query_cov": 0.9090909090909091, "subject_cov": 0.8695652173913043, "identity": 0.65, "score": 49.3, "evalue": 5.65e-07, "valid": false, "uniref50_id": "UniRef50_A0AB38W775" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W775" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5011.0954999999985, "isoelectric_point": 11.999967765808105 }, "id": "KKNAAMEMLJ_631", "locus": "GCA000027325_00615" }, { "type": "cds", "sequence": "contig_1", "start": 345305, "stop": 345631, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATCCAACAGCTTTACAATGATGCAATTGCAAATAAAAATCAATTGTCTGCAATTGCTCTAACTAAATTCCAACCAACTACTAGTTTTTGTTATCAAGTATTCCAAGTACAACTCCCTACCCTTTCTTTTTACAGTGAATATCAAAAGGAAAAAACCCATATCAAAGGTTTTAATCCTGAGCTTTTTTTAATTAATCAAGTTGGTCAAAAACAACTCAATGATCCTTTGGTTTTACGACCTACTAGTGAGATTGCTTTTTGCAACTTATGGAAAAAACAAGAGTTATCTTACCATGATCTACCTTTAATTTATAACCAGTGA", "aa": "MEIQQLYNDAIANKNQLSAIALTKFQPTTSFCYQVFQVQLPTLSFYSEYQKEKTHIKGFNPELFLINQVGQKQLNDPLVLRPTSEIAFCNLWKKQELSYHDLPLIYNQ", "aa_hexdigest": "82917a68bea6990deb1af9ee76d9273a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12659.35619999999, "isoelectric_point": 6.70810375213623 }, "id": "KKNAAMEMLJ_632", "locus": "GCA000027325_00616" }, { "type": "cds", "sequence": "contig_1", "start": 345812, "stop": 346633, "strand": "+", "frame": 2, "gene": "proS", "product": "proline--tRNA ligase", "db_xrefs": [ "COG:COG0442", "COG:J", "EC:6.1.1.15", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101", "KEGG:K01881", "SO:0001217", "UniRef:UniRef50_P47525", "UniRef:UniRef90_P47525" ], "nt": "TTGAAAAGTGAAAGTGAAAAATTTGCAGGTGCTAAAAACACATGGACAATAGAAGCAATTATGCCTGATGGACAAAGTTTACAATGTGCCACTAGCCATGATTTAGGTGACACTTTTACAAAGAGTTTTACTATCAGCTATCAGAGTAAAACTAACCAAAAAATGACTCCAAGTAGTTTTAGTTGTGGGATGTCAACTAGGATCTTAGGAGCAATTTTTTTAACCCACAGCGATGATTATGGTTTGGTTTTACCTTGGTATCTAGCAAGTAAACAAGTCAAGTTATACCTGTTTGATAAAAACAATAACCCTAAAACAAGAGCTTTAGCTTTTTTAGTGAAGGATTTTTTAGAAAAACTCAAAATTCGCTTTAGTTTTATAGAAATTAACAATCAACTAGGTAAACAACTTTTAAAAGGAGAAATAGAAGGTATTCCATTACAGATGATTGTTGATAATGAAAAAACTATTAACATCTTCAACCGCTTAACACGTTTAAAAACCAGCTTAACATTTGCAAATCTCCAAACTGAATTTGTTAATTTAGTTAACAACTACCATACAGAGATGTATAGAAAAGCAAATGATTTAGTTGAACAAAAACTAGCAAGAGTACAAACTTTAAAGGAAATTGAACAAGCATTCAAAAATAAAAAGGCTGTTTTATGTACCGTGAAGTTAACTGGTGAACTTGAACAACACTTAAAGACAAAATACCAAGTTAGTGTTAGGTGTGTTTTTAAAAAGTCAGATGTAACACAAAACTGTCCTTTTACAAATCAACCTTGTTTTGATTCAGTTTTAATTGCACGTGCTTACTAA", "aa": "MKSESEKFAGAKNTWTIEAIMPDGQSLQCATSHDLGDTFTKSFTISYQSKTNQKMTPSSFSCGMSTRILGAIFLTHSDDYGLVLPWYLASKQVKLYLFDKNNNPKTRALAFLVKDFLEKLKIRFSFIEINNQLGKQLLKGEIEGIPLQMIVDNEKTINIFNRLTRLKTSLTFANLQTEFVNLVNNYHTEMYRKANDLVEQKLARVQTLKEIEQAFKNKKAVLCTVKLTGELEQHLKTKYQVSVRCVFKKSDVTQNCPFTNQPCFDSVLIARAY", "aa_hexdigest": "30b484fd8dcc2c15dd577c282f1d0ae7", "start_type": "TTG", "rbs_motif": null, "genes": [ "proS" ], "truncated": "5-prime", "pseudogene": { "start": 345512, "stop": 346633, "observations": { "mutation": [ 345629, 345692, 345755 ], "stop": [ 345629, 345692, 345755 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47525", "query_cov": 1.0, "subject_cov": 0.5652173913043478, "identity": 0.996, "score": 536.0, "evalue": 2.84e-189, "gene_start": 1, "gene_end": 273, "reference_start": 211, "reference_end": 483, "reference_sequence": "MTKKTQDLTSWYDQLLVKAKLICHGEVKGTVCFLNNSWGLWMEIQQLYNDAIANKNQLSAIALTKFQPTTSFCYQVFQVQLPTLSFYSEYQKEKTHIKGFNPELFLINQVGQKQLNDPLVLRPTSEIAFCNLWKKQELSYHDLPLIYNQWTQVFRAEKNTRPFLRNSEFYWQETHGLFVDQSQSEQAAISFWNLYQDLIINKLCIPAFVGLKSESEKFAGAKNTWTIEAIMPDGQSLQCATSHDLGDTFTKSFTISYQSKTNQKMTPSSFSCGMSTRILGAIFLTHSDDYGLVLPWYLASKQVKLYLFDKNNNPKTRALAFLVKDFLEKLKIRFSFIEINNQLGKQLLKGEIEGIPLQMIVDNEKTINIFNRLTRLKTSLTFANLQTEFVNLVNNYHTEMYRKANDLVEQKLARVQTLKEIEQAFKNKKAVLCTVKLTGELEQHLKTKYQVSVRCVFKKSDVTQNCPFTNQPCFDSVLIARAY" }, "paralog": false, "identity": 0.9919571045576407, "score": 738.0, "evalue": 3.83e-271, "description": "Internal stop codon at 345629, 345692, 345755. Nonsense mutation around 345629, 345692, 345755.", "psc": { "uniref90_id": "UniRef90_P47525", "gene": "proS", "product": "proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_P47525", "cog_id": "COG0442", "cog_category": "J", "kegg_orthology_id": "K01881", "go_ids": [ "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47525", "EC:6.1.1.15", "UniRef:UniRef50_P47525", "COG:COG0442", "COG:J", "KEGG:K01881", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ] }, "pscc": { "uniref50_id": "UniRef50_P47525", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47525" ], "product": "Proline--tRNA ligase" } }, "id": "KKNAAMEMLJ_633", "locus": "GCA000027325_00617" }, { "type": "cds", "sequence": "contig_1", "start": 347040, "stop": 347180, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAAAAGAAACTGTTGATTTTATCTATGACATCTATATTTATGGCAAAAAGCCGTGTGATATTGGTTATTCTAATTCCACCTATTACAAAAAACTAAACAAAGCAGCTAATAGTTTTTTTGACCATTTTGTTTGA", "aa": "MLKKETVDFIYDIYIYGKKPCDIGYSNSTYYKKLNKAANSFFDHFV", "aa_hexdigest": "f2deb3d58c90f77abbe22626051eb867", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5480.226300000001, "isoelectric_point": 8.734063911437985 }, "id": "KKNAAMEMLJ_634", "locus": "GCA000027325_00618" }, { "type": "cds", "sequence": "contig_1", "start": 347215, "stop": 347448, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCAATTCTCAACGTACAACGAATTCAAAATAACCAAGTCACTGAGTACACAATGAGTCCAGTGCGTAATTTTGCAAACACAAAAGATGTATATTTTGATGCGCAATTAACAAATATAGAAAGCAAAATTGATAGTAGTAGGGCTCAAATTCACCTTACTATTGCACTAAAATACAACACTAATCTCCCTGATAATATCTTTCAAGCCCACTTCAGTTTAGGCAATTGA", "aa": "MAAILNVQRIQNNQVTEYTMSPVRNFANTKDVYFDAQLTNIESKIDSSRAQIHLTIALKYNTNLPDNIFQAHFSLGN", "aa_hexdigest": "6d6c6b0bfab9efe4f3dd4b95d4f5effe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8772.763199999994, "isoelectric_point": 6.703386116027832 }, "id": "KKNAAMEMLJ_635", "locus": "GCA000027325_00619" }, { "type": "cds", "sequence": "contig_1", "start": 347659, "stop": 347796, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATACTGTAGCTAGTGAATTGTATGCAACAGTAATTAAAGAAACAATCAACTTTGGCAACATGATTAAGATTAATGCACTTGATGGTAGTAAACAGCTAACTAGTAGCCAAGGAAGTTTTAAATACAGTTGA", "aa": "MFDTVASELYATVIKETINFGNMIKINALDGSKQLTSSQGSFKYS", "aa_hexdigest": "f9c6c28bf20885274471409d04788bbd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4949.565500000001, "isoelectric_point": 5.970674705505371 }, "id": "KKNAAMEMLJ_636", "locus": "GCA000027325_00620" }, { "type": "cds", "sequence": "contig_1", "start": 347800, "stop": 348009, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGACTATAGGAATTTAGAACAACTTGATGAGGTGAGAAACTTGATCAATATCAGTTTTGACAAACCAGTTCAAATAGTAAATGTTGATGTCAAAATTCACTATGTTCCAACTAAAGGTAGGTTACAAGAGATAAAACAACAAGGTGAGTTTGAAAATAACCTTGATGTTAATGAAAAGCTTAAACTCAATTTAATAGGCAATTGA", "aa": "MYDYRNLEQLDEVRNLINISFDKPVQIVNVDVKIHYVPTKGRLQEIKQQGEFENNLDVNEKLKLNLIGN", "aa_hexdigest": "c574042e20a6344067741eb2cd5cc493", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8157.187999999998, "isoelectric_point": 5.236087608337402 }, "id": "KKNAAMEMLJ_637", "locus": "GCA000027325_00621" }, { "type": "cds", "sequence": "contig_1", "start": 348100, "stop": 348258, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGCGCTACAGTAGGTAATGATTTCTACACAATTATAGCCAACAACCAGTTTAAGTACGAAACACCTTTAGATGATCTTGAACAAAATGACTTTTTTGAGGTAAATTATTTACCTGTTTACAGTACATACAACTTTAGTGATTTAACTCAGTAA", "aa": "MIGATVGNDFYTIIANNQFKYETPLDDLEQNDFFEVNYLPVYSTYNFSDLTQ", "aa_hexdigest": "ee5c7edd3f97380bf48e0d9f469d5c24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6080.559900000001, "isoelectric_point": 4.0500284194946286 }, "id": "KKNAAMEMLJ_638", "locus": "GCA000027325_00622" }, { "type": "cds", "sequence": "contig_1", "start": 348261, "stop": 348773, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG286", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47528", "UniRef:UniRef90_P47528" ], "nt": "ATGATTTTTTCAATTAGTAAACGAAAGTTAATTTGCGGATTTTTGCTAGTTATTTTAACAATAGGTGGGGTTTTGGGTGGTGTTTATTTAGTTACTAAAAACAACAAGGATAACTACCAAAATGAAAGTAATTTCAACAATCAAGAACAAATTAGTAAAATCCCTAATTTCAAAGCTATTGGTCCTGAAACACAACGTATCTTAAGAGAAAGAAACTATCCTTTAGATGATAGTGGTTATTATGTTTATAAGTATGGTGAAATTAATAGGTATCTAAGAAATGAAAGTGAACTAGATGAATTAATTAACTATAGAGTGATGGTACCTTCACTTAAATTACATCACAAAAGGGTTAATTTTGATAAGGCATTTCTTGAAAGTAAATTAAGAAAATGGATTATCAAAGCAATTAAGCAACATAACTATTTCCAACACTTTGAAAATGAACCAAATTTAAGAGTTCAATATAACATGAATATTCCAGCACAAAAAATTGATGTTAATGCTGTTTGA", "aa": "MIFSISKRKLICGFLLVILTIGGVLGGVYLVTKNNKDNYQNESNFNNQEQISKIPNFKAIGPETQRILRERNYPLDDSGYYVYKYGEINRYLRNESELDELINYRVMVPSLKLHHKRVNFDKAFLESKLRKWIIKAIKQHNYFQHFENEPNLRVQYNMNIPAQKIDVNAV", "aa_hexdigest": "e5b99606183d7ea264581e8c67c2b01e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47528", "query_cov": 1.0, "subject_cov": 0.8673469387755102, "identity": 1.0, "score": 342.0, "evalue": 1.34e-118, "valid": true, "product": "Uncharacterized protein MG286", "uniref50_id": "UniRef50_P47528", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47528", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47528" ], "product": "Uncharacterized protein MG286" }, "genes": [], "id": "KKNAAMEMLJ_639", "locus": "GCA000027325_00623" }, { "type": "tRNA", "sequence": "contig_1", "start": 349128, "stop": 349202, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 70.7, "nt": "AGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCA", "db_xrefs": [], "id": "KKNAAMEMLJ_12", "locus": "GCA000027325_00624" }, { "type": "cds", "sequence": "contig_1", "start": 349282, "stop": 349620, "strand": "+", "frame": 1, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDG5", "UniRef:UniRef90_UPI00030561CF" ], "nt": "GTGCCTAAGGTGGTATTGGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCTTTTGATCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATACAGGAAAGATGGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTGGTGGGGCTACATCTCCTCGCAAAGCCCTCACCATTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCCAAAAAGCCACTGAAACATAAGAACAGTAGTGGGGAGGTGAAGTTGGATGCGAGTGGGGAGTTTACTGAGGCCTGA", "aa": "MPKVVLEYHNLNNQVVKESLEVEATDSFDPTQRLQKDSPMKDTGKMGEKLQETMSSMSGGGATSPRKALTIEVERGSQSDSLLKNDFAKKPLKHKNSSGEVKLDASGEFTEA", "aa_hexdigest": "4620b486cacdb0578c58546d8d72feb7", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00030561CF", "query_cov": 1.0, "subject_cov": 0.8549618320610687, "identity": 1.0, "score": 209.0, "evalue": 2.68e-68, "valid": true, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "genes": [], "id": "KKNAAMEMLJ_640", "locus": "GCA000027325_00625" }, { "type": "cds", "sequence": "contig_1", "start": 349634, "stop": 349804, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCGACATTGATGTTAGAAAAAGTGGGGAGTGGGGAAGAAGAAGAAGAAGAAAGGGAAGCAGTGTGGGAGTTGGAGAAGGGTTTGTTGTTGCCGATTCAGGGGAGAAAGTCTAAGTCGCCCCCATCCCCTTCTCTCTTGCTATTTGATCAGTAG", "aa": "MIIDLTPTLMLEKVGSGEEEEEEREAVWELEKGLLLPIQGRKSKSPPSPSLLLFDQ", "aa_hexdigest": "b3d90d5a9d0c1f407c8c6e8559eb1e7b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6308.1889999999985, "isoelectric_point": 4.403794288635253 }, "id": "KKNAAMEMLJ_641", "locus": "GCA000027325_00626" }, { "type": "cds", "sequence": "contig_1", "start": 349923, "stop": 350327, "strand": "+", "frame": 3, "gene": null, "product": "Random genomic clone esh10, partial cds", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "nt": "GTGTTGCCTAGTAGCAATAGTAGTCAACAGTTTAATCCCAATTCAGATGATAATAAAGTCACTCAAGGTGGTGGCTCCCCAGCCAAAAAAACAACCACCTATTCCTTTTTACCCAATTCCATCAGTCCCACCAGTGACTGGATCAACGCATTGACTTTTACTAACAAAAATAACCCGCAGCGCAATCAACTGTTGCTCAGAAGCTTACTAGGAACTATCCCGGTATTGATCAATAAGAGTGGAACGGGAGATGAGTTTACCCATACGAGTGAGCAGAAATGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTTATCAACTAAATAAAAAACTTATTAGCTTATTTTTATTAGGTTTTTACTTATTTAATAGTTAA", "aa": "MLPSSNSSQQFNPNSDDNKVTQGGGSPAKKTTTYSFLPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGTGDEFTHTSEQKWDKTNEKDGNLPGFGEVNGGFYQLNKKLISLFLLGFYLFNS", "aa_hexdigest": "1d1e67e9d7d8068ed0ed8f787e51722a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D20171", "query_cov": 0.8656716417910447, "subject_cov": 0.9830508474576272, "identity": 0.8190000000000001, "score": 184.0, "evalue": 4.93e-58, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_Q49206" }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" }, "genes": [], "id": "KKNAAMEMLJ_642", "locus": "GCA000027325_00627" }, { "type": "cds", "sequence": "contig_1", "start": 350605, "stop": 350778, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI002962482B" ], "nt": "GTGAAAAAATATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACGAAGTACTGAAAAATTTAATTCTTGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCTAAAGAGAAGGGTAAAACGCAAAGGGAGGTGCATCTGGGTAGTGGGCAAGCAAATCAGTGA", "aa": "MKKYQLDVPNVSSNMNEVLKNLILEQPLTKYTLNSSLAKEKGKTQREVHLGSGQANQ", "aa_hexdigest": "a1f10d14d2ad559051e3001852436a04", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 350452, "stop": 350858, "observations": { "deletion": [ 350803 ], "mutation": [ 350596, 350776 ], "stop": [ 350596, 350776, 350842 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002962482B", "query_cov": 1.0, "subject_cov": 0.456, "identity": 0.86, "score": 91.7, "evalue": 1.29e-22, "gene_start": 1, "gene_end": 57, "reference_start": 48, "reference_end": 103, "reference_sequence": "MLFKSTQVKVKYESSGSNNISFDSTNNKPSYIVEFTNSTNIGIKWSVVKKYKLDVPNVSNEMNEVLKNLILEQPLTKYTLNSSLAKEKGKSQIEVHLGGQASQWQSMRNQHGPKQQSQPKCHYRV" }, "paralog": false, "identity": 0.7851851851851852, "score": 182.0, "evalue": 7.78e-61, "description": "Internal stop codon at 350596, 350776, 350842. Frameshift due to deletion around 350803. Nonsense mutation around 350596, 350776.", "psc": { "uniref90_id": "UniRef90_UPI002962482B", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002962482B", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6415.2705000000005, "isoelectric_point": 9.45623874664307 }, "id": "KKNAAMEMLJ_643", "locus": "GCA000027325_00628" }, { "type": "cds", "sequence": "contig_1", "start": 350876, "stop": 351055, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTCCTGACCAATTTATCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACAGAAGCAACAACGGCAAAAAATGATGCGCCCAGTGTTTCTGGAGGGGGATCAGACACCACTTCAAAATTTAAAAGTTACCTCAACACCAAGCAAGCGTTAG", "aa": "MSPDQFINQLMGPSRAKGRIVVGGVQQKQQRQKMMRPVFLEGDQTPLQNLKVTSTPSKR", "aa_hexdigest": "1baf61847c9e82aa32cf79a2ae53b5a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6667.725600000002, "isoelectric_point": 11.480223655700684 }, "id": "KKNAAMEMLJ_644", "locus": "GCA000027325_00629" }, { "type": "cds", "sequence": "contig_1", "start": 351307, "stop": 351507, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "TTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAGTTGATCTTCAGTGGCTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGA", "aa": "MGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQLIFSGFKAGSVGYDSSSSSSSSSSTKDQALA", "aa_hexdigest": "18866cb2694ea23847f413b01128f43e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 68, "aa_cov": 1.0, "hmm_cov": 0.9705882352941176, "evalue": 1.223428839244514e-19, "score": 65.61052703857422, "start": 1, "stop": 66 } ], "seq_stats": { "molecular_weight": 6947.354700000003, "isoelectric_point": 5.974028205871583 }, "id": "KKNAAMEMLJ_645", "locus": "GCA000027325_00630" }, { "type": "cds", "sequence": "contig_1", "start": 351553, "stop": 351825, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "GTGACCAACGACACGGGGCTAAATGGTCCGATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATACAAATTCAAGTGGTTCATCAGGAACCATTAAAACGGCTTATCCGGTGAAAAACACAGAAAAATCAACTGTCAAGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATGAGGGGATTGGGGTTAGAAATATTTTTATGAGTTTAAAACAAGGTAATGCAGATAACTAG", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSGNHTNSSGSSGTIKTAYPVKNTEKSTVKINSLINATPLNSYGDEGIGVRNIFMSLKQGNADN", "aa_hexdigest": "44a1c0e830c99af79b32178a7a1fc294", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 76, "aa_cov": 0.8444444444444444, "hmm_cov": 0.9868421052631579, "evalue": 2.233026729443789e-24, "score": 81.11206817626953, "start": 3, "stop": 78 } ], "seq_stats": { "molecular_weight": 9504.334599999996, "isoelectric_point": 6.524286460876465 }, "id": "KKNAAMEMLJ_646", "locus": "GCA000027325_00631" }, { "type": "cds", "sequence": "contig_1", "start": 351791, "stop": 352054, "strand": "-", "frame": 2, "gene": null, "product": "DUF237 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCE2A4C", "UniRef:UniRef90_UPI002FCE2A4C" ], "nt": "ATGGATGTCAAGTTCATAGTTGATGGTGATTTGGATTATAACTTAGTTCAAAATACTGATTATCCAGGTTCTCTATTTCAATTTAAAGACAATCAACTACTTTTCACTTTACACGTAAAAGAACAGATAAAAGTTCAAGATGGTAAATTTATGGATTTACTTAAACAGCAAAACTTACATAATTTGGATTTAAGAAATGGGGCTACAAAACCTCCAGTTGTTGATTTAGCTAGTTATCTGCATTACCTTGTTTTAAACTCATAA", "aa": "MDVKFIVDGDLDYNLVQNTDYPGSLFQFKDNQLLFTLHVKEQIKVQDGKFMDLLKQQNLHNLDLRNGATKPPVVDLASYLHYLVLNS", "aa_hexdigest": "229ef08e7f663adb17b609e23f8042f9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 351791, "stop": 352147, "observations": { "mutation": [ 352066, 352099 ], "stop": [ 352066, 352099 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE2A4C", "query_cov": 1.0, "subject_cov": 0.7372881355932204, "identity": 0.9890000000000001, "score": 177.0, "evalue": 2.84e-56, "gene_start": 1, "gene_end": 87, "reference_start": 32, "reference_end": 118, "reference_sequence": "MDIKYQGGYKLTGLFSWFAPFSGIPSSWNGEMDVKFIVDGDLDYNLVQNTDYPGSLFQFKDNQLLFTLHVKEQIKVQDGKFMDLLKQQNLHNLDLRNGATKPPVIDLASYLHYLVLNS" }, "paralog": false, "identity": 0.9661016949152542, "score": 233.0, "evalue": 8.42e-81, "description": "Internal stop codon at 352066, 352099. Nonsense mutation around 352066, 352099.", "psc": { "uniref90_id": "UniRef90_UPI002FCE2A4C", "product": "DUF237 domain-containing protein", "uniref50_id": "UniRef50_UPI002FCE2A4C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE2A4C", "UniRef:UniRef50_UPI002FCE2A4C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FCE2A4C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCE2A4C" ], "product": "DUF237 domain-containing protein" } }, "id": "KKNAAMEMLJ_647", "locus": "GCA000027325_00632" }, { "type": "cds", "sequence": "contig_1", "start": 352892, "stop": 353035, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGGATTTTGAAGTTGATTATGAAGGTGGTAACAACACTGTTAACTTGCAATTTGATTTAAAAGCCCAAACTTCTAATTTTTTGAATTTACAAGAGTTGCAAAATAGCTTTAATGGTAACGATCTAAATACCCAATTGTTTTGA", "aa": "MDFEVDYEGGNNTVNLQFDLKAQTSNFLNLQELQNSFNGNDLNTQLF", "aa_hexdigest": "80f911f4ede0e2ef14fbb3ea82dc4d4c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 49, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.9980447776397105e-10, "score": 35.492130279541016, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 5401.750099999999, "isoelectric_point": 4.0500284194946286 }, "id": "KKNAAMEMLJ_648", "locus": "GCA000027325_00633" }, { "type": "cds", "sequence": "contig_1", "start": 352975, "stop": 353247, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "TTGCCTAAAGTTCCACAGAATTTTTTTAAATTCCTTAATGAAAATTTCTTTCCACAATTGCATCCAAAAGGTTTAAACATTACCGAAAGTATTGTTGAAATATTTAATCAATACAACTTAAAGAAAATTCAATTAAAAGACTTTAATCTTGAATTGGTTAAAAAAATGACATTGTTATTAAAGATAAGATTCGTTATAGCTTTGAAATCAAAATGGATTTTGAAGTTGATTATGAAGGTGGTAACAACACTGTTAACTTGCAATTTGATTTAA", "aa": "MPKVPQNFFKFLNENFFPQLHPKGLNITESIVEIFNQYNLKKIQLKDFNLELVKKMTLLLKIRFVIALKSKWILKLIMKVVTTLLTCNLI", "aa_hexdigest": "a5b6e595e9bbd00c50da02b9aa8c7db6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 79, "aa_cov": 0.8555555555555555, "hmm_cov": 1.0, "evalue": 6.125397408779862e-19, "score": 63.3598518371582, "start": 2, "stop": 78 } ], "seq_stats": { "molecular_weight": 10677.95569999999, "isoelectric_point": 10.009957695007323 }, "id": "KKNAAMEMLJ_649", "locus": "GCA000027325_00634" }, { "type": "cds", "sequence": "contig_1", "start": 353299, "stop": 353682, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_029329864.1", "SO:0001217", "UniParc:UPI0000557DDF", "UniRef:UniRef100_UPI0000557DDF", "UniRef:UniRef50_UPI0000557DDF", "UniRef:UniRef90_UPI0000557DDF", "PFAM:PF03086.20" ], "nt": "TTGAAAGCTATCAAAAAATCTCGTTTTAAAATTAAAACTTTTCTCTTTGTCTCTTTTGCATCAACTTTAACTGCTGGTGTACTTATTGTCCCTTCTATCTATAATGGCTCAGCTGCAAATTTAAGTCAACCTAGTCTAAGAAAATCAAGTACAACAAATAGTAGAGAAAATAGTATTCTACTCCAACAGCAAACAGCTATAACAGAGCAAGAAGAAAACTATATTGCTAAAGAGAAAGATTATGATAAAAACTTTGACTTATTTCAAGAGAAATTTAAGAATGAATTTTATCCTAGGAACTTTAATGTTATAGATATTTTTCATCTTTTAAGTGGTTTTAAATCAGGTATTAAAAAAACAGTTGATTTATTAAATAGGCTGTAA", "aa": "MKAIKKSRFKIKTFLFVSFASTLTAGVLIVPSIYNGSAANLSQPSLRKSSTTNSRENSILLQQQTAITEQEENYIAKEKDYDKNFDLFQEKFKNEFYPRNFNVIDIFHLLSGFKSGIKKTVDLLNRL", "aa_hexdigest": "2e89320a9aa095bb4cee907537be8b99", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557DDF", "ncbi_nrp_id": "WP_029329864.1", "uniref100_id": "UniRef100_UPI0000557DDF", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557DDF", "RefSeq:WP_029329864.1", "UniRef:UniRef100_UPI0000557DDF" ] }, "ips": { "uniref100_id": "UniRef100_UPI0000557DDF", "uniref90_id": "UniRef90_UPI0000557DDF" }, "psc": { "uniref90_id": "UniRef90_UPI0000557DDF", "uniref50_id": "UniRef50_UPI0000557DDF" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557DDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557DDF" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 56, "aa_cov": 0.4409448818897638, "hmm_cov": 0.9642857142857143, "evalue": 9.180324849684582e-10, "score": 33.32433319091797, "start": 72, "stop": 127 } ], "seq_stats": { "molecular_weight": 14578.544099999986, "isoelectric_point": 9.696512413024902 }, "id": "KKNAAMEMLJ_650", "locus": "GCA000027325_00635" }, { "type": "cds", "sequence": "contig_1", "start": 354024, "stop": 354476, "strand": "+", "frame": 3, "gene": "cypl", "product": "ABC transporter thiamine pyrophosphate-binding lipoprotein p37/Cypl", "db_xrefs": [ "GO:0005886", "GO:0009897", "GO:0030976", "SO:0001217", "UniRef:UniRef50_P75371", "UniRef:UniRef90_Q49410" ], "nt": "GTGCTTTTCAAAAAATTTACTTGGGTCATTCCAAGTTTGTTTTTAACAATTATTTCAACATCTTTACTTATTAGTTGTGCAACTAAAAGCGATAACACCTTAATATTTAATATTTCACTTGATCATAACGCTGATACATCAATAGAAAAATTCTTTACTGTTTTTTCAAAAAAACTTAGTGGAAAATTGAATAAAAAAATTAATGTTAACTTTAATATAGTTGATGATTCCTTTACAAAAATTAACAATATTCAAGCTAATAAAGCAGATTTTGCTTTTGTTAATTCACAAGCTATTGCTTCAAATAATTGGTTTGGCTATACGCCATTGATACAAACTTTAACAACAGCTTTTAAAGAAGATTTGGAGCTTGATTATTATGAAGATGGTAATTTACAAAAAAAAGCTGAAAAAACGAATTTGCTTTTTCTAAGTCCACCTTACAAAGAATGA", "aa": "MLFKKFTWVIPSLFLTIISTSLLISCATKSDNTLIFNISLDHNADTSIEKFFTVFSKKLSGKLNKKINVNFNIVDDSFTKINNIQANKADFAFVNSQAIASNNWFGYTPLIQTLTTAFKEDLELDYYEDGNLQKKAEKTNLLFLSPPYKE", "aa_hexdigest": "1cd7cdb8a50970041935547c2b2a0dea", "start_type": "GTG", "rbs_motif": null, "genes": [ "cypl" ], "truncated": "3-prime", "pseudogene": { "start": 354024, "stop": 354779, "observations": { "mutation": [ 354474, 354495, 354609, 354624 ], "stop": [ 354474, 354495, 354609, 354624 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49410", "query_cov": 1.0, "subject_cov": 0.4076086956521739, "identity": 1.0, "score": 291.0, "evalue": 1.16e-96, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MLFKKFTWVIPSLFLTIISTSLLISCATKSDNTLIFNISLDHNADTSIEKFFTVFSKKLSGKLNKKINVNFNIVDDSFTKINNIQANKADFAFVNSQAIASNNWFGYTPLIQTLTTAFKEDLELDYYEDGNLQKKAEKTNLLFLSPPYKEWDDIKQKWTGNRYDFLYEPSKLVSFYRSMILITGSASEITAIKKAWNEKNWNQFMKFGIGHGQTNSASRFELPDLLFRKHFAKNYPGLQNAINSDPDKFAVVRGREIGINKNIKIVFDDANSFSWTQNIKGSKRPFYTPIDPNDRLEILTYSDPLLYDIGIVSNNLSRIYQKAIGEIFIELAQSSEDLYGPSIGYNGYKMINDFEKEVVEIIEKTYGK" }, "paralog": false, "identity": 0.9800796812749004, "score": 487.0, "evalue": 5.39e-176, "description": "Internal stop codon at 354474, 354495, 354609, 354624. Nonsense mutation around 354474, 354495, 354609, 354624.", "psc": { "uniref90_id": "UniRef90_Q49410", "gene": "cypl", "product": "ABC transporter thiamine pyrophosphate-binding lipoprotein p37/Cypl", "uniref50_id": "UniRef50_P75371", "go_ids": [ "GO:0005886", "GO:0009897", "GO:0030976" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49410", "UniRef:UniRef50_P75371", "GO:0005886", "GO:0009897", "GO:0030976" ] }, "pscc": { "uniref50_id": "UniRef50_P75371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75371" ], "product": "High affinity transport system protein p37" } }, "id": "KKNAAMEMLJ_651", "locus": "GCA000027325_00636" }, { "type": "cds", "sequence": "contig_1", "start": 354636, "stop": 354848, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTGGAATTGGTCATGGACAAACAAATTCAGCTTCACGTTTTGAGCTACCTGATCTTTTATTTAGAAAACATTTTGCTAAAAATTATCCCGGATTACAAAATGCAATTAATTCTGATCCCGATAAATTTGCCGTAGTTAGAGGAAGAGAGATAGGTATAAATAAAAACATCAAGATTGTTTTTGATGATGCTAATTCATTTTCTTGA", "aa": "MKFGIGHGQTNSASRFELPDLLFRKHFAKNYPGLQNAINSDPDKFAVVRGREIGINKNIKIVFDDANSFS", "aa_hexdigest": "cfa480c30db29582fa09225b1ac9e7b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7853.800199999999, "isoelectric_point": 9.598198127746581 }, "id": "KKNAAMEMLJ_652", "locus": "GCA000027325_00637" }, { "type": "cds", "sequence": "contig_1", "start": 354939, "stop": 355130, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGACATTGGTATTGTTAGCAACAATTTATCAAGGATATATCAAAAAGCTATTGGTGAAATTTTTATTGAGTTAGCACAATCAAGTGAAGATCTATATGGGCCTTCAATTGGTTATAACGGCTATAAAATGATTAATGATTTTGAGAAAGAAGTTGTTGAAATAATTGAAAAAACCTATGGAAAATAA", "aa": "MYDIGIVSNNLSRIYQKAIGEIFIELAQSSEDLYGPSIGYNGYKMINDFEKEVVEIIEKTYGK", "aa_hexdigest": "76a8ba54349b9eba9d4038b1ede7ec4c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7199.0813, "isoelectric_point": 4.532932472229004 }, "id": "KKNAAMEMLJ_653", "locus": "GCA000027325_00638" }, { "type": "cds", "sequence": "contig_1", "start": 355120, "stop": 355452, "strand": "+", "frame": 1, "gene": "p29", "product": "putative ABC transporter ATP-binding protein p29", "db_xrefs": [ "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47532", "UniRef:UniRef90_P47532" ], "nt": "ATGGAAAATAAACCAATTCTTTCTTTTGAAAAAGTATCAATAATATACAAAAAAGCTCCACTTTTGCAAAACATTAGTTTTAAAGTAATGGCAAAGGAAAATGTTTGCTTATTAGGTAAGTCTGGAGTTGGAAAATCGAGCCTTTTAAACAGTGTTACTAATACAAAAATAGTTAAAAGTGGGTTAGTTTATTTTGATGGTGTTGCTTCAAACAAAAAGGAATACAAAAAACTGAAAAAACAGTGCAGTTATCTGGATCAAATACCAAATTTAATTGACACTGATTATGTATATGAAGCAATTTTAAGATCTGCTAAACAAAAATTAACTTGA", "aa": "MENKPILSFEKVSIIYKKAPLLQNISFKVMAKENVCLLGKSGVGKSSLLNSVTNTKIVKSGLVYFDGVASNKKEYKKLKKQCSYLDQIPNLIDTDYVYEAILRSAKQKLT", "aa_hexdigest": "1d7b689a5bce4d2c65bbf50590f5ae49", "start_type": "ATG", "rbs_motif": null, "genes": [ "p29" ], "truncated": "3-prime", "pseudogene": { "start": 355120, "stop": 355755, "observations": { "mutation": [ 355450, 355483 ], "stop": [ 355450, 355483 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47532", "query_cov": 1.0, "subject_cov": 0.4489795918367347, "identity": 1.0, "score": 214.0, "evalue": 1.04e-68, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MENKPILSFEKVSIIYKKAPLLQNISFKVMAKENVCLLGKSGVGKSSLLNSVTNTKIVKSGLVYFDGVASNKKEYKKLKKQCSYLDQIPNLIDTDYVYEAILRSAKQKLTWLQKLICFEPKWIKDKILAILKEVNLNDYVSCIIKDLSAGQKQRVEIAKLFFKSPKLLLVDEPTTGLDPLTASKIMDLITDFVKREKITLVFVTHDIDLALKYSTRIIALKNHALVLDRLTEKLTKEQLYKIYDN" }, "paralog": false, "identity": 0.990521327014218, "score": 402.0, "evalue": 5.94e-145, "description": "Internal stop codon at 355450, 355483. Nonsense mutation around 355450, 355483.", "psc": { "uniref90_id": "UniRef90_P47532", "gene": "p29", "product": "Probable ABC transporter ATP-binding protein p29", "uniref50_id": "UniRef50_P47532", "cog_id": "COG1120", "cog_category": "PH", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47532", "UniRef:UniRef50_P47532", "COG:COG1120", "COG:PH", "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P47532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47532" ], "product": "Probable ABC transporter ATP-binding protein p29" } }, "id": "KKNAAMEMLJ_654", "locus": "GCA000027325_00639" }, { "type": "cds", "sequence": "contig_1", "start": 355675, "stop": 355857, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTAATTACTGATTTTGTGAAAAGAGAAAAAATAACTTTGGTTTTTGTAACACATGATATAGATTTAGCACTGAAATATAGCACCAGAATTATAGCACTTAAAAATCATGCTTTAGTGTTAGATAGATTAACAGAAAAACTAACAAAAGAACAACTTTATAAAATTTATGATAACTAA", "aa": "MDLITDFVKREKITLVFVTHDIDLALKYSTRIIALKNHALVLDRLTEKLTKEQLYKIYDN", "aa_hexdigest": "88f04202242222c9f20f895f5f2b5583", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7121.301099999999, "isoelectric_point": 8.026007652282715 }, "id": "KKNAAMEMLJ_655", "locus": "GCA000027325_00640" }, { "type": "cds", "sequence": "contig_1", "start": 356164, "stop": 356361, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAAAAATACTTTAAATTGCTGTTTTCAAAAAATGTAGTAATGTATTTTGTATTCCATAAAAAGCTAAACCAGCTAATCGTTAGTGTAGCTGCTAGTTGTTTATTAAATAAATTACTAAATAAAAAACCAAATAAAAGTACGGGAAATGCTCTTAAAACTATAGTTATTAACCTGATCGGTAGAGCAATGTAA", "aa": "MLKKYFKLLFSKNVVMYFVFHKKLNQLIVSVAASCLLNKLLNKKPNKSTGNALKTIVINLIGRAM", "aa_hexdigest": "ea0a6ac4d6a749e99d97b28b6105ad82", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7398.0519, "isoelectric_point": 10.522352409362792 }, "id": "KKNAAMEMLJ_656", "locus": "GCA000027325_00641" }, { "type": "cds", "sequence": "contig_1", "start": 356543, "stop": 356701, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTTGATCTTGTATTAATTCCATTAGTTGTTTTAATAACCTTTCTAATTTTTATAGAAGTTGTTGTTTTTTTATTATCAAGTTTTGTTTTTGAGAAAAATAGTGAAGATTTAAGACCAATTTTAAAAACAACAGTTATTGAAAAACGAAAGTGA", "aa": "MQFDLVLIPLVVLITFLIFIEVVVFLLSSFVFEKNSEDLRPILKTTVIEKRK", "aa_hexdigest": "bdb16a9757650d9cf74e495780c5d553", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6095.363299999998, "isoelectric_point": 6.09100284576416 }, "id": "KKNAAMEMLJ_657", "locus": "GCA000027325_00642" }, { "type": "cds", "sequence": "contig_1", "start": 356720, "stop": 357214, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGTAGTACTAATTAGTCTTAGTTTAGCCAATTTAGTGACTATTGATTACAGAATTAACGATGCTGAATTTTTACAAGATTTTTTTAACCAGTTTTTTCAGTTAAAAAGTAATCTTTTTTCAAGTAATGATCCTAATATAAACCCAATTTTAATGTTAGTTAAACTTACAACTCAAGCTATTAGTTTAATTAGCTTGGTTGTAATCTTTTCTATTCTCTTTGGTTTTATATCATGTAATTTATTTAAAAAGAGATTTTCAATTAGTTTTAAGATCTTATTACTTTTTGTTAGAGTAGTTCCTAGTATTCTGTTGTTTAGGCTACTAGATCCTTTATTTCTAGAAGCAAAAACAACTATTATTTTAGTCTTACTAATTAATCATGGTTCAAGTTATGGCCAATTGATGTCAATTAACTTTAATAAGGCAAATCAAAATATCATCAATAACTATAAAAATCATGGTATGACAAAAGGTTTTATTTTATGA", "aa": "MFIVVLISLSLANLVTIDYRINDAEFLQDFFNQFFQLKSNLFSSNDPNINPILMLVKLTTQAISLISLVVIFSILFGFISCNLFKKRFSISFKILLLFVRVVPSILLFRLLDPLFLEAKTTIILVLLINHGSSYGQLMSINFNKANQNIINNYKNHGMTKGFIL", "aa_hexdigest": "33d04578d4270d64157949ac4975ca5a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18743.166199999992, "isoelectric_point": 9.721719551086426 }, "id": "KKNAAMEMLJ_658", "locus": "GCA000027325_00643" }, { "type": "cds", "sequence": "contig_1", "start": 357221, "stop": 357478, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTTGAAAATAAACCTAATTTAATAAACATTACCAGTGATGCTTATGATAGTGTAATTAGGGATTTAATTTTGTTTGGTAGTTTTGGCGGTTCAATTATTGGTAGTAGAATTACTAATTTTTTTGAAAGAGCTCAATTTGATAATCTAGGTTCTGTTACAATCCCATTAATGGTTTATCTAATTGCAATAGAAATAATTTTTCTTTCAGTTAGATTAACTAGAATTTCAGTTTTTAAGAACTACCTTTACTAA", "aa": "MLVENKPNLINITSDAYDSVIRDLILFGSFGGSIIGSRITNFFERAQFDNLGSVTIPLMVYLIAIEIIFLSVRLTRISVFKNYLY", "aa_hexdigest": "3ebb81aa84edb897d1ee358526302905", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9652.191599999991, "isoelectric_point": 6.081283378601074 }, "id": "KKNAAMEMLJ_659", "locus": "GCA000027325_00644" }, { "type": "cds", "sequence": "contig_1", "start": 357475, "stop": 357891, "strand": "-", "frame": 3, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "COG:COG0816", "COG:JLK", "EC:3.1.-.-", "EC:3.1.21.10", "GO:0000967", "GO:0004518", "GO:0005737", "GO:0006364", "RefSeq:WP_010869411.1", "SO:0001217", "UniParc:UPI00001395BC", "UniRef:UniRef100_Q9ZB76", "UniRef:UniRef50_Q9ZB76", "UniRef:UniRef90_Q9ZB76" ], "nt": "GTGAAATATATATTAGCAATTGATTTTGGCTTGAAAAAAATAGGTACAGCAATTGCCAATACACTAGATAAATATCCTTCTGCATTTCATGTTTTTGAAGTAAAAAATAATTTCAAAACTGCTGTTAACAACTTATTTTTGCGAATTAAAAACGATGGGTATGAACTTGAAAAAATAGTGATAGGTTTTCCCAAATTTCACTATTATTCTGATATTCAAAAGGCAATTAAATCATTTAAACAACTTTTAGAAAAACGTTTTAATTTGCCAATAATTTTGGTTGATGAAAGTAATACTACAAGTGCTGTTAAAGATAAGCTTATAACAATGGATTTAAAACATAAGGATTTTAAAAAGGCTAAAGATACATTAGCTGCAGTTTTAATATTAGAGCGCTTCTTTCAAAACTATCATTAG", "aa": "MKYILAIDFGLKKIGTAIANTLDKYPSAFHVFEVKNNFKTAVNNLFLRIKNDGYELEKIVIGFPKFHYYSDIQKAIKSFKQLLEKRFNLPIILVDESNTTSAVKDKLITMDLKHKDFKKAKDTLAAVLILERFFQNYH", "aa_hexdigest": "b121948389378b0af34e9015063facda", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001395BC", "ncbi_nrp_id": "WP_010869411.1", "uniref100_id": "UniRef100_Q9ZB76", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001395BC", "RefSeq:WP_010869411.1", "UniRef:UniRef100_Q9ZB76" ] }, "ips": { "uniref100_id": "UniRef100_Q9ZB76", "uniref90_id": "UniRef90_Q9ZB76" }, "psc": { "uniref90_id": "UniRef90_Q9ZB76", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.-.-", "3.1.21.10" ], "uniref50_id": "UniRef50_Q9ZB76", "cog_id": "COG0816", "cog_category": "JLK", "go_ids": [ "GO:0000967", "GO:0004518", "GO:0005737", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB76" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "KKNAAMEMLJ_660", "locus": "GCA000027325_00645" }, { "type": "cds", "sequence": "contig_1", "start": 357891, "stop": 358394, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATAGTTCAATTTTTAAAGTTGAGTTAAAAGAATTGCAACAAAGGCTAGATAAATTTATCTTACCTGAAAAAATTACCCAATTAAGAGATGCATCTGATACTTTATTAGCTTTAAAAAATGATATTAACCAGTTAAAAACAAAAAACTATAAAGTATCACAGCAAGCTTTAGCTTTATCAATTAAAAAGCAATTATTATCCTTAGTAGATGAAAATAAAAGTTATGTAATTGCCACTTTTAATGACGTAGAACCTAAACTATTGCTACAAACACTACATGATGTTTTCAATCAAAATCAAACTAAAAATTTCTTGATAATTAATCAATTCAATGAAAGTAATTCATTTATTGTTATAGGAAATAAAACTACCACCATTATTGAAAAATTAAGAAATAGTTTTAATTTAAAGGGCGGAGGCAATGATAAGTTATTTAGAGGTTCTTTTCAGGATAATGTTACCCCTCAAAAGCTTAATGAATTGTTTCAAAATAAATAG", "aa": "MIDSSIFKVELKELQQRLDKFILPEKITQLRDASDTLLALKNDINQLKTKNYKVSQQALALSIKKQLLSLVDENKSYVIATFNDVEPKLLLQTLHDVFNQNQTKNFLIINQFNESNSFIVIGNKTTTIIEKLRNSFNLKGGGNDKLFRGSFQDNVTPQKLNELFQNK", "aa_hexdigest": "f7ea46884a2a110b42bf89cc4437c65e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19203.827, "isoelectric_point": 9.371011543273926 }, "id": "KKNAAMEMLJ_661", "locus": "GCA000027325_00646" }, { "type": "cds", "sequence": "contig_1", "start": 358497, "stop": 358784, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "TTGAATCGTCATTTAACAAGAAATGAACTTGAGAAAGTAGAAAATAAAATTCGCTCTTTGATTAAACAAAAAATAAGCTCAAAAGAGATTTTTACTGATTTTGAAGGGAGTCAAAAACTAAATGCAATTGCTTATTTTGAAGAGGAATATTCTCAACATGAAATATTAAGAGTGATCCGCTTTGGTGATTATAGTGTTGAGTTGTGTGGTGGCACTCATGTAGCTAACACTGCTTCAATTGAAGATTGTTTTATTACTGATTTCTATTCTTTAGGAGCTGGAAGATGA", "aa": "MNRHLTRNELEKVENKIRSLIKQKISSKEIFTDFEGSQKLNAIAYFEEEYSQHEILRVIRFGDYSVELCGGTHVANTASIEDCFITDFYSLGAGR", "aa_hexdigest": "4484f6e68ec478f8e7ae77ca3d693164", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 31, "aa_cov": 0.3263157894736842, "hmm_cov": 1.0, "evalue": 1.3694726646991537e-12, "score": 42.56776809692383, "start": 56, "stop": 86 } ], "seq_stats": { "molecular_weight": 10951.18359999999, "isoelectric_point": 5.577348899841308 }, "id": "KKNAAMEMLJ_662", "locus": "GCA000027325_00647" }, { "type": "cds", "sequence": "contig_1", "start": 358914, "stop": 359312, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCAAACATCGTTCTATCTCTAAGCAAAATAACCAAACTATAAATTTTGAAAAACAAAATATTAATTTAGTTAATTTCAAAACTAAAAGTACTTTTTTTTATCACAAAAATAAAATTAATGCTAAGGTAATTGGTCTTTTTGATGAAAATTATTTACCAGTTAAAGAACTTAATAATCAAAGTGGTTATGTAGTTTTTGACCAAACAGTTTTATATGCTACTTCTGGAGGACAGAGATATGATGAAGGAAGTTGCATTAATCATTCTAATAATAATGATCAAAAAATCAGTTTTCAAGGTGTATTTAAAGGACCTAATAAACAACACTTCCACTACTTTTTAGTAGGTAGTTTTAAACTCAATGATCAAGTAACTTTATCACATGATGAAACTTGA", "aa": "MAKHRSISKQNNQTINFEKQNINLVNFKTKSTFFYHKNKINAKVIGLFDENYLPVKELNNQSGYVVFDQTVLYATSGGQRYDEGSCINHSNNNDQKISFQGVFKGPNKQHFHYFLVGSFKLNDQVTLSHDET", "aa_hexdigest": "ef1292213d18431e6bc67b5fc9f8f670", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15240.800699999993, "isoelectric_point": 9.163229942321777 }, "id": "KKNAAMEMLJ_663", "locus": "GCA000027325_00648" }, { "type": "cds", "sequence": "contig_1", "start": 359331, "stop": 359765, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGAACAGAGTTATTTTCGGATTATTGCTGATCACTTTAAAGCAATCACTTTTACCATTTCAGAAGGAGTTTTACCTGGTCCTAATGAGAGAAATTATGTAGTAAGAAGACTTTTAAGACGTGCTTTAATAGCTTGTAAGAAATTGCAATTAAACTTAGCATTTATTGAAAAGATAATAGATGAAATCATCGCTTCATATGAGAATTATTATCAACATTTAAAAGCTAAAAATGAAACTGTTAAACAGGTAGTTTTAAAAGAGATTAATGCATTTAATAAAACGATTGATTTAGGTTTAGTGCTGTTTGAAAAAAGTGTTAAAAACAATACTCTAACTCCCCAATTAACATTTCAATTGAACGAAACATACGGTTTTCCTGTTGAAATAATAAGAGAACTAGTTAATCAAAAAGGTTTAACTATTGATTGA", "aa": "MKEQSYFRIIADHFKAITFTISEGVLPGPNERNYVVRRLLRRALIACKKLQLNLAFIEKIIDEIIASYENYYQHLKAKNETVKQVVLKEINAFNKTIDLGLVLFEKSVKNNTLTPQLTFQLNETYGFPVEIIRELVNQKGLTID", "aa_hexdigest": "23ca99a13a5b762f994d0c51192e8f18", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16717.290799999988, "isoelectric_point": 9.161424827575683 }, "id": "KKNAAMEMLJ_664", "locus": "GCA000027325_00649" }, { "type": "cds", "sequence": "contig_1", "start": 360183, "stop": 360365, "strand": "-", "frame": 1, "gene": null, "product": "alanine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920MQJ2" ], "nt": "GTGGGTTTTACTTCAAGACATCAAACTTTGTTTGAGATGCTTGGAAATTTTTCAATCGGTGATTATTTTAAAACAGAAGCAATTGATTTTGCTTTTGATCTTTTAGTTAATTATTATCAGCTAGATCCTAAGCGTTTTTATATCACTGTTTATGAAGATGATGAAACTACTTATAAAAGATGA", "aa": "MGFTSRHQTLFEMLGNFSIGDYFKTEAIDFAFDLLVNYYQLDPKRFYITVYEDDETTYKR", "aa_hexdigest": "7d6e2918c56b5049e14b9f42f329ec5e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920MQJ2", "query_cov": 0.9166666666666666, "subject_cov": 0.9649122807017544, "identity": 0.564, "score": 65.5, "evalue": 5.5e-13, "valid": false, "product": "alanine--tRNA ligase", "uniref50_id": "UniRef50_A0A920MQJ2" }, "pscc": { "uniref50_id": "UniRef50_A0A920MQJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920MQJ2" ], "product": "alanine--tRNA ligase" }, "genes": [], "id": "KKNAAMEMLJ_665", "locus": "GCA000027325_00650" }, { "type": "cds", "sequence": "contig_1", "start": 360810, "stop": 361385, "strand": "-", "frame": 1, "gene": null, "product": "putative MG293-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75367" ], "nt": "ATGCATAACAAGCAATTGCTTTTAGCACATAGGGGTTATTCATTCATTGCTCCAGAAAACACCAAACTAGCATTTGATTTAGCTTTTGAATATTGTTTTGATGGAATAGAGCTTGATGTTCATTTAACTAAAGATGAACAGTTAGTTATCATTCATGATGAGACAACATTGAGAACCGCATTAGTTAATAAGGAGGTTGAGTTTGAATCATTAGTTAGTTTAAAAAGAGATGATCATAGTGCTTTTTTTCACCTTAAAATTCAATTTCAATCGATCCTAACTTTAAAAGAGTTCTTAGATCTTTATTTAGATAAATTTAAGTTAATCAATATTGAGATTAAAACTGATCAAAAACCATATTTAGGAATTGAAAAGAAGCTTGTTGACCTAGTTAAAGGTTATGGTAAAAAAGCAATAGATAAGATCTTGTTTTCATCCTTTAACTTTGAATCTTTGCAAAAAGTTTATGATTTAGATAATAGTTACAAAAAAGGTTTTTTATTTTGGACTAAAAAACAGTTTGAAACAATTAGTACAGCTAGAATCCAAAAGATTTGTCAATTCCTCCACCCATGA", "aa": "MHNKQLLLAHRGYSFIAPENTKLAFDLAFEYCFDGIELDVHLTKDEQLVIIHDETTLRTALVNKEVEFESLVSLKRDDHSAFFHLKIQFQSILTLKEFLDLYLDKFKLINIEIKTDQKPYLGIEKKLVDLVKGYGKKAIDKILFSSFNFESLQKVYDLDNSYKKGFLFWTKKQFETISTARIQKICQFLHP", "aa_hexdigest": "052932c87959439b70799977b107973e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021153946", "query_cov": 1.0104712041884816, "subject_cov": 0.8041666666666667, "identity": 0.56, "score": 192.0, "evalue": 1.91e-58, "valid": false, "product": "glycerophosphodiester phosphodiesterase family protein", "uniref50_id": "UniRef50_P75367" }, "pscc": { "uniref50_id": "UniRef50_P75367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75367" ], "product": "Uncharacterized protein MG293 homolog" }, "genes": [], "id": "KKNAAMEMLJ_666", "locus": "GCA000027325_00651" }, { "type": "cds", "sequence": "contig_1", "start": 361399, "stop": 361581, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTGCTGAAGTTGGTAAGAAATATACAGTTGCAGGACAAAGTAATACTAGTAACACTGGTTATCAAATTATCGCTGCTATTTGTTTATCCATTGCTTTGTTTGGTTTGATATGTGGATCAATTGTTTTTATCTCAAATAGCATGGAAATTAAACGATTAAATAAAGCTTCATACCGTTGA", "aa": "MTAEVGKKYTVAGQSNTSNTGYQIIAAICLSIALFGLICGSIVFISNSMEIKRLNKASYR", "aa_hexdigest": "cf0d1d4f4608e3693ae2dd3f8843f71b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6447.482499999996, "isoelectric_point": 9.445279121398926 }, "id": "KKNAAMEMLJ_667", "locus": "GCA000027325_00652" }, { "type": "cds", "sequence": "contig_1", "start": 361591, "stop": 362022, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGATGTATGTGTTTCAAAGCACTTTTGCTTATTACCTTTTACAGATGTTGCAAAATGCATTTTTAGCGCCTGTAGTATTAGTAACTGTAATTGGGGGAATTAGAACTTATGCACTGAGAAGTGCTGTTAGTGTTTATCTTTGTAGATTAGCAGATAAGTGTAAAAGCTATATCTTGTTTTTAATGATATGTACTGTTCTTGGTATTGTATTTGTTCTAGCATTTATTCTACTTGGTTTTGTACAAACAAATTCTGCTAACATCACTTTAATTACTTTCTCTTCCATTCTCTATATCTTTACTGGTATCTTATCATGGGGAATGGTTACAGTCAGATATAACCAAATAGGTGAAATTGATATTGGTAAAAATAACTATGCATCTAGTGTTGGTTTACTTAGTTTTATTGGTTTTTCTACTGATGGATGA", "aa": "MGMYVFQSTFAYYLLQMLQNAFLAPVVLVTVIGGIRTYALRSAVSVYLCRLADKCKSYILFLMICTVLGIVFVLAFILLGFVQTNSANITLITFSSILYIFTGILSWGMVTVRYNQIGEIDIGKNNYASSVGLLSFIGFSTDG", "aa_hexdigest": "f65a846cbf0473432450004e1e6a3c54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15778.59289999998, "isoelectric_point": 8.842435264587401 }, "id": "KKNAAMEMLJ_668", "locus": "GCA000027325_00653" }, { "type": "cds", "sequence": "contig_1", "start": 362050, "stop": 362268, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAATTACCAGCATTTTCTATCCATCAGGAGGTAGTGAGAATGCTGATTCAAAACCATTTGCTGCATACGCTTTTATCATTGCCATAATGCTAGTAATTACTGGATTTACAGTTTTGTTTTTTGTTAAGGAAAAACCAATTGAAAAACAAAGTCAAACCACTTTAGTAAGTTTTAAACGCAATTTAAACCAGATTTTAGTGACCTTAAAAAACTGA", "aa": "MIITSIFYPSGGSENADSKPFAAYAFIIAIMLVITGFTVLFFVKEKPIEKQSQTTLVSFKRNLNQILVTLKN", "aa_hexdigest": "2bef7fdc8ff824425dca24460644bae4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8045.4123999999965, "isoelectric_point": 9.522770118713382 }, "id": "KKNAAMEMLJ_669", "locus": "GCA000027325_00654" }, { "type": "cds", "sequence": "contig_1", "start": 362494, "stop": 362802, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAGTGACAACAAAAAAAGGATTTAAAAAACGCTTAAAGGATTTTTCAAAACAACAAATTCTTGCCTTAATTATTTTGGGAGCAATTGATGTATTTGTAATTGCTGCACCCTATTATGTTAAGAATGTTGTTCCTAACCTCCACTTGTACTTAGGAATTACAGAGGATGAAGTTGCTACTGTTACTTCTATCATTGGTTATGTAACACTTGCCACTCAACTACCAGGAGGGTTTTTAACAAATCGCTTCAGTTCTAGAAAATTGTTATTTCTATCAGCAATTACTACTGGAGCAATTACTTTTTGA", "aa": "MIVTTKKGFKKRLKDFSKQQILALIILGAIDVFVIAAPYYVKNVVPNLHLYLGITEDEVATVTSIIGYVTLATQLPGGFLTNRFSSRKLLFLSAITTGAITF", "aa_hexdigest": "8a988d55027ac4c158b3bd172fa6033d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11208.199799999986, "isoelectric_point": 9.850978660583493 }, "id": "KKNAAMEMLJ_670", "locus": "GCA000027325_00655" }, { "type": "cds", "sequence": "contig_1", "start": 362920, "stop": 363156, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20258.4" ], "nt": "TTGAAAACAACAATTTTATTGGATCAATTTAACTGGTTGTATACACCAAAGCAACTTCCTAGTCAAGTTCTGGTAAGAATTAGACATGCTCAAAAACCAGAAATTGCAAAGTTGAAATTATTATCAGATAATAAACTGGAAATAACATTTAAAAATCCTGTTATAAGTGTTGCATCTGGACAGTTTGGTGTATTATATACACTTGATCAAATTTGTTTAGGAGCAGGATTAATTTAA", "aa": "MKTTILLDQFNWLYTPKQLPSQVLVRIRHAQKPEIAKLKLLSDNKLEITFKNPVISVASGQFGVLYTLDQICLGAGLI", "aa_hexdigest": "c1d2718396eccacb5089be333c7ea795", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20258.4", "name": "tRNA_Me_trans_C", "length": 77, "aa_cov": 0.9743589743589743, "hmm_cov": 0.987012987012987, "evalue": 4.481100471445626e-20, "score": 66.9324722290039, "start": 3, "stop": 78 } ], "seq_stats": { "molecular_weight": 8781.335199999998, "isoelectric_point": 9.517612648010253 }, "id": "KKNAAMEMLJ_671", "locus": "GCA000027325_00656" }, { "type": "cds", "sequence": "contig_1", "start": 363427, "stop": 363732, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03054.22" ], "nt": "ATGATTCAAAGTTTCAAAAAAGGGTTAACCCCAAATCCAGACATCTGGTGTAATCGTTTTATTAAGTTTGGTTTATTGCATGATTTTTGTAAGCAAATTAACCCTAATTCTCTTTTTGCAACTGGTCATTATGCCAAAATAAACATGATAGAAAATCAGCCTTTGCTTTCTATTCCTAAAGATACCAATAAAGATCAAACTTATTTTTTAGCAAATGTTAAAAAAGAACAATTTCAGAATGTTATTTTTCCTTTAGCAGATTTAAAAAAAATAACAGTGAGAAATATTGCTAGAGAAAATAATTGA", "aa": "MIQSFKKGLTPNPDIWCNRFIKFGLLHDFCKQINPNSLFATGHYAKINMIENQPLLSIPKDTNKDQTYFLANVKKEQFQNVIFPLADLKKITVRNIARENN", "aa_hexdigest": "5c07ac57deafb152956cd58897f2ad07", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 102, "aa_cov": 0.9900990099009901, "hmm_cov": 0.9901960784313726, "evalue": 1.3481666273088084e-35, "score": 117.92342376708984, "start": 1, "stop": 100 } ], "seq_stats": { "molecular_weight": 11753.529599999983, "isoelectric_point": 9.554230690002441 }, "id": "KKNAAMEMLJ_672", "locus": "GCA000027325_00657" }, { "type": "cds", "sequence": "contig_1", "start": 363751, "stop": 364023, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGTCAATTATTGCAAAAACAGTTTTTATAGGTTTAAGCGGTGGTGTTGATTCTGCTGTTAGTGCTTTACTTTTAAAAAAGCAATACCAAGAAGTTATTGGTGTTTTTATGGAATGTTGGGATGAGACACTTAATAATGATTTTTATGGTCATAAGAAAATAAATAATAACAAATCAGGTTGTTCATCTTTTCAAGACTTCCAACAGGCTAAAAAAATCGCTAATTCTTTAGGAATTAAGTTAATAAAAAAAAACTTAATTGAAGCTTATTGA", "aa": "MSIIAKTVFIGLSGGVDSAVSALLLKKQYQEVIGVFMECWDETLNNDFYGHKKINNNKSGCSSFQDFQQAKKIANSLGIKLIKKNLIEAY", "aa_hexdigest": "500ce6b67aeecff802c7361978185654", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 79, "aa_cov": 0.8444444444444444, "hmm_cov": 0.7341772151898734, "evalue": 1.3746351548749768e-08, "score": 29.25231170654297, "start": 5, "stop": 80 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 84, "aa_cov": 0.9333333333333333, "hmm_cov": 0.8928571428571429, "evalue": 6.466857585521227e-21, "score": 69.98857116699219, "start": 7, "stop": 90 } ], "seq_stats": { "molecular_weight": 10005.48289999999, "isoelectric_point": 8.928629493713384 }, "id": "KKNAAMEMLJ_673", "locus": "GCA000027325_00658" }, { "type": "cds", "sequence": "contig_1", "start": 364026, "stop": 364415, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG296", "db_xrefs": [ "RefSeq:WP_009885876.1", "SO:0001217", "UniParc:UPI00001395B2", "UniRef:UniRef100_P47538", "UniRef:UniRef50_P75364", "UniRef:UniRef90_P47538" ], "nt": "ATGAAACCACAACTTATAGCTTTTAAAAAATTTTTACAAACTGAATTTCAAGCGGTTGATTTTGAGACTTTTCGCATTAACTTTAATTTGTGTTTAAAACGAGAACAAGACAATATTGTTATTTATGAAGATGATGATTATGATGACCAACCATTTTTTTTCAAACCAATGTTAAGTGATGGTTTTTTTATCCAAACTGAAGTAATTAAACAGCTAGATTATCTAGCAAAAGTTGTAGAAAATCCTAAAGATAGTGATCAACAGTGCTGTCAAAATTTTTATGAAGCATTAATAGTGTTTATTTCTGCACTTGCTATTACAAAAGGCATTAATCCTAATAGATTTCACCAAAGATTAGTAAACAGGTTTGCTATTCACGCTGTTTATTAA", "aa": "MKPQLIAFKKFLQTEFQAVDFETFRINFNLCLKREQDNIVIYEDDDYDDQPFFFKPMLSDGFFIQTEVIKQLDYLAKVVENPKDSDQQCCQNFYEALIVFISALAITKGINPNRFHQRLVNRFAIHAVY", "aa_hexdigest": "83893479caceb5d4407b303c3b3b4750", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001395B2", "ncbi_nrp_id": "WP_009885876.1", "uniref100_id": "UniRef100_P47538", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001395B2", "RefSeq:WP_009885876.1", "UniRef:UniRef100_P47538" ] }, "ips": { "uniref100_id": "UniRef100_P47538", "uniref90_id": "UniRef90_P47538" }, "psc": { "uniref90_id": "UniRef90_P47538", "product": "Uncharacterized protein MG296", "uniref50_id": "UniRef50_P75364" }, "pscc": { "uniref50_id": "UniRef50_P75364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75364" ], "product": "Uncharacterized protein MG296 homolog" }, "genes": [], "id": "KKNAAMEMLJ_674", "locus": "GCA000027325_00659" }, { "type": "cds", "sequence": "contig_1", "start": 364535, "stop": 364867, "strand": "-", "frame": 2, "gene": null, "product": "Cell division protein FTSY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "nt": "ATGAATGAATTGCAAAAAATTTATCAAATTATTCAAAAGGTAAGTGGAAGTGAACCTAGTGAAACACTTTTAGTTTTAGATGGTACAGTAGGTCAAACAGGACTATCACAAGCAAAGGTATTTAATGAATTTTCCAAACTAACAGGGATTGTTTTAACTAAAATGGATGGTTCTGCTAAGGGTGGAATTATTTTAGCTATTAAAGATATGTTTAACCTGCCTGTTAAACTGATAGGTTTTGGTGAAAAAACTAGTGATTTAGCTATCTTTGATCTGGAAAAATATGTTTTAGGTTTACTTAATAACTTAAACTTAGATAATAAAGAAAATTAG", "aa": "MNELQKIYQIIQKVSGSEPSETLLVLDGTVGQTGLSQAKVFNEFSKLTGIVLTKMDGSAKGGIILAIKDMFNLPVKLIGFGEKTSDLAIFDLEKYVLGLLNNLNLDNKEN", "aa_hexdigest": "2c89b26b0ef6df32ef5a2a99fa59dd5e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PYR8", "query_cov": 0.9636363636363636, "subject_cov": 0.8346456692913385, "identity": 0.6509999999999999, "score": 139.0, "evalue": 2.35e-40, "valid": false, "product": "Cell division protein FTSY", "uniref50_id": "UniRef50_A0A3B0PYR8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PYR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "product": "Cell division protein FTSY" }, "genes": [], "id": "KKNAAMEMLJ_675", "locus": "GCA000027325_00660" }, { "type": "cds", "sequence": "contig_1", "start": 365018, "stop": 365575, "strand": "-", "frame": 2, "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "db_xrefs": [ "COG:COG0552", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0006614", "GO:0009306", "GO:0016887", "KEGG:K03110", "SO:0001217", "UniRef:UniRef50_P47539", "UniRef:UniRef90_P47539" ], "nt": "ATGGGCTTTTTAAGCAAGTTAATTGCCAAACTAAAACCAAAAAAATCAGTTGCTAAACAGCTTAAAGAAGAAGTTGAAAAACAAAGCCTTTTTCAAACCAATAATAAAACTTACTATCAGGGTTTGAAAAAATCTGCTACAACTTTCGCTAAAACTATTAATGAACTGTCAAAACGATATGTTAATGTTGACGAACAGTTTAAAGAAAATCTATTTGAAGGGCTAGTTTTGCTTGATGTTGGTTATCATGCTGCAAACAAAATTTGTGATGCTATTATTGAACAGATCAAGCTAAACAGAATTACAGATTTTCAGCTCATTAAAGAGCTAATTATTGACCAAATTATTGTTTATTACATCCAAGATAAACTCTTTGATACTGATTTAATAGTTAAACCTAACTTTACAAATGTTTATCTCTTTGTTGGTGTTAATGGAGTTGGTAAAACAACTACTTTAGCTAAGATAGCGGATTTTTTCATAAAACAAAATAAACGTGTTCTACTTGTTGCAGGTGATACTTTTAGAGCAGGAGCCATTGAACAACTTAATCAGTGA", "aa": "MGFLSKLIAKLKPKKSVAKQLKEEVEKQSLFQTNNKTYYQGLKKSATTFAKTINELSKRYVNVDEQFKENLFEGLVLLDVGYHAANKICDAIIEQIKLNRITDFQLIKELIIDQIIVYYIQDKLFDTDLIVKPNFTNVYLFVGVNGVGKTTTLAKIADFFIKQNKRVLLVAGDTFRAGAIEQLNQ", "aa_hexdigest": "3ce5e31761c7177dd696a15c2d827d00", "start_type": "ATG", "rbs_motif": null, "genes": [ "ftsY" ], "truncated": "5-prime", "pseudogene": { "start": 364715, "stop": 365575, "observations": { "mutation": [ 365020 ], "stop": [ 365020 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47539", "query_cov": 1.0, "subject_cov": 0.5346820809248555, "identity": 1.0, "score": 350.0, "evalue": 2.8e-119, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MGFLSKLIAKLKPKKSVAKQLKEEVEKQSLFQTNNKTYYQGLKKSATTFAKTINELSKRYVNVDEQFKENLFEGLVLLDVGYHAANKICDAIIEQIKLNRITDFQLIKELIIDQIIVYYIQDKLFDTDLIVKPNFTNVYLFVGVNGVGKTTTLAKIADFFIKQNKRVLLVAGDTFRAGAIEQLNQWAKLLNCDIVLPNPKEQTPAVIFRGVKKGIDDKYDFVLCDTSGRLQNKLNLMNELQKIYQIIQKVSGSEPSETLLVLDGTVGQTGLSQAKVFNEFSKLTGIVLTKMDGSAKGGIILAIKDMFNLPVKLIGFGEKTSDLAIFDLEKYVLGLLNNLNLDNKEN" }, "paralog": false, "identity": 0.9965034965034965, "score": 554.0, "evalue": 3.18e-202, "description": "Internal stop codon at 365020. Nonsense mutation around 365020.", "psc": { "uniref90_id": "UniRef90_P47539", "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P47539", "cog_id": "COG0552", "cog_category": "U", "kegg_orthology_id": "K03110", "go_ids": [ "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0006614", "GO:0009306", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47539", "EC:3.6.5.4", "UniRef:UniRef50_P47539", "COG:COG0552", "COG:U", "KEGG:K03110", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0006614", "GO:0009306", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47539" ], "product": "Signal recognition particle receptor FtsY" } }, "id": "KKNAAMEMLJ_676", "locus": "GCA000027325_00661" }, { "type": "cds", "sequence": "contig_1", "start": 365582, "stop": 366418, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13304.12", "PFAM:PF02463.25" ], "nt": "TTGTTAGATGAACAACGTGCTAAATTAGTTTCAGCGCGGGAAGGAAAAATTAGGTTTGAAAATACAATCCAAAATATCACTGAAAAGATTAACAGTGTTTACAAAATGACAATGGAGTTTGCTATTGCTAATCACAACAAACCAGTTAAACTCTCTTCTATGCAAGCCCATAACAAGATTGCTAAACTACAAAATCAATTGAATGAAATGGGGGTAATTAACATGGAATCAATTGCTGAGATAAGTGAAAAGCAAAAGCGCTTTGATGATATTAACGCTGAATATGAATCAGCTCAACAAGCAGTTGAAAATTTGCAAAAAGCTATTACTGAAATTGATGAAATTGCTAGCAATGAGTTTGATCAACTGATCCAAAAATTAAATCAAGAGTTGCCAAAAACTTTCAAATATCTATTTGGTGGTGGTTCTTGCCAAATTCGTTACACTGATCCTAGTAATGTATTGGTTTCAGGGATAGATGTCTTTGCAAATCCTCCTGGTAAAAATATTGCCAACTTAATGTTGTTATCAGGCGGGGAAAAAACTTTAGTTGCACTTTCTGTTTTATTTAGTATTTTGAAAGTAAGTGCTTTTCCACTTGTTATTTTAGATGAAGCGGAAAGTGCACTTGATCCTGCTAATGTGGAGCGCTTTGCTAACATCATTAAAACTGCTAGCAAAAACACCCAATTTTTAATTATTACCCATCGTCAAGGTACAATGATGAAATGTGACATGTTATTGGGTGCTGCAATGCAAACTAAAGGTGTAACTAAAACCTTTGCAGTTGAACTTGAAAATGCTGAAAAATATGTATCTGAAAATGATTCTAATTAA", "aa": "MLDEQRAKLVSAREGKIRFENTIQNITEKINSVYKMTMEFAIANHNKPVKLSSMQAHNKIAKLQNQLNEMGVINMESIAEISEKQKRFDDINAEYESAQQAVENLQKAITEIDEIASNEFDQLIQKLNQELPKTFKYLFGGGSCQIRYTDPSNVLVSGIDVFANPPGKNIANLMLLSGGEKTLVALSVLFSILKVSAFPLVILDEAESALDPANVERFANIIKTASKNTQFLIITHRQGTMMKCDMLLGAAMQTKGVTKTFAVELENAEKYVSENDSN", "aa_hexdigest": "70422232939b4f45122da1d49838dcc0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13304.12", "name": "AAA_21", "length": 194, "aa_cov": 0.6942446043165468, "hmm_cov": 0.8247422680412371, "evalue": 2.6291182550228463e-10, "score": 35.874908447265625, "start": 44, "stop": 236 }, { "id": "PF02463.25", "name": "SMC_N", "length": 198, "aa_cov": 0.7122302158273381, "hmm_cov": 0.5, "evalue": 1.7056575646520053e-29, "score": 97.88218688964844, "start": 61, "stop": 258 } ], "seq_stats": { "molecular_weight": 30970.167400000024, "isoelectric_point": 5.435876655578612 }, "id": "KKNAAMEMLJ_677", "locus": "GCA000027325_00662" }, { "type": "cds", "sequence": "contig_1", "start": 366527, "stop": 367861, "strand": "-", "frame": 2, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261", "KEGG:K03529", "SO:0001217", "UniRef:UniRef50_P47540", "UniRef:UniRef90_P47540" ], "nt": "TTGAAAGAGTTGGAACTTGCTGTTTTGGTTGGGGAATATCTCCAAGCACAAACTGAACTTGATAAGTTTAATTTTCAGATTAACAGTAGTGAACATGACTTTAAAATACACGAACCCCAACTAGAGTTGTTAGAAGAACAGATTGTTATCTTTAATAGTCGCTTTCATAGTGCTGATATGCAATCTAATGAATTGCAAAAAGAGTTACAAGATATTTACCAAAAGATCAATGAATTGGAACAACGTAAAGTCATTATTGATGTGCAGTTAAGACAAGGTTTTTCACAAAAAGATGAAAAGCAAAAAGCTGCAGCTTTAAAAAAACTAATTTTAGTTGATCAAACCCAGCTTGATGGTTTTGAAAACCAACTATCAAATTCCAAAACAACCATCACTGATCTTGAAAAACTAATTAACGAACAAAAATCACTTGTTGATCAGATAAAGTTACAGATTGAAAAAAATACTGCTGATTTAATTTACCAACGTTCCTTAAAAACAATTATTGAACTCCAAACTAATGAGCTTAAAAAAACTAACAATGCCAATATCTTAGTTAAAAATGCTAATGCTTTAACAGGAATTTTAAATACACTAGGAACATTTCTTAAATTTGATAAGCAGTATGAAAAAGCTATCTTAAAAGCACTTGGAAAATCAATTGGTTACCTAGTTGTTAACAACAATAATGCTGCCATCCAAGCAATAGATTTTTTAGTCAAAAATGAGATAGGTAAAGTAACTTTTTTACCTTTAGATGATGTTGCTAGTGATACTAAAATCACAAACGAACACATGGAAATTTTAAAGCAGCTTGATGGTTTTCTTGGTGTTTGTAGTGATCATGTTAAGTGTGATCCATTGTTTCAACCAGTTGTAAATACTCTTTTAGCACAAGTTATTATTGCTAAAGATTTAAATAGTGCCATTAACCTTTCTAACTATACTTACAAGTTATATAGGATCGTTACTTTAGATGGTGAGACAGTTTATGCAGGGGGAATTATCAATGGTGGATTTGAAAAAACAAATCTTAGTGATGGGTATTTATCATCAGCTTCACTTGATAATGAACAGAACATTAACAAACTAGAAAACAACGAACGAGAGTTAAAAAAGGAGCTTACAGAACTAGAAGTAAAGCTAGATGAAATGAACAGAAAACTCAAGTATGAAGAGTTGTTACAAGCCAAGTTTATAGAGCGTATTGTTCAAATTAAAAAAATTATTTTAGAGCTCAAAATGGAATATGAACAACTTACTAACACAACTTTTGATGGTAAAAAAGCTGTTGCTAGTGAAGCTGAATTAATCCATTCATTAAATAGTGCTTGA", "aa": "MKELELAVLVGEYLQAQTELDKFNFQINSSEHDFKIHEPQLELLEEQIVIFNSRFHSADMQSNELQKELQDIYQKINELEQRKVIIDVQLRQGFSQKDEKQKAAALKKLILVDQTQLDGFENQLSNSKTTITDLEKLINEQKSLVDQIKLQIEKNTADLIYQRSLKTIIELQTNELKKTNNANILVKNANALTGILNTLGTFLKFDKQYEKAILKALGKSIGYLVVNNNNAAIQAIDFLVKNEIGKVTFLPLDDVASDTKITNEHMEILKQLDGFLGVCSDHVKCDPLFQPVVNTLLAQVIIAKDLNSAINLSNYTYKLYRIVTLDGETVYAGGIINGGFEKTNLSDGYLSSASLDNEQNINKLENNERELKKELTELEVKLDEMNRKLKYEELLQAKFIERIVQIKKIILELKMEYEQLTNTTFDGKKAVASEAELIHSLNSA", "aa_hexdigest": "795a2de1cb2bb1cdd200c0f7cea4c51a", "start_type": "TTG", "rbs_motif": null, "genes": [ "smc" ], "truncated": "both", "pseudogene": { "start": 366224, "stop": 368161, "observations": { "mutation": [ 366529, 368083 ], "stop": [ 366529, 368083 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47540", "query_cov": 1.0, "subject_cov": 0.45213849287169044, "identity": 0.998, "score": 763.0, "evalue": 3.12e-268, "gene_start": 1, "gene_end": 444, "reference_start": 224, "reference_end": 667, "reference_sequence": "MVFLKRFRAYGFKSYADEITIDFTHSMTGIVGPNGSGKSNVVDALKWVLGERSMKHLRSKSGDDMIFFGSKDKPASKLAEIELTFDNSNRLLHDSRKEISVMRRVYRGSGQSEYFINSNPATLKEISGIFADIGLEKGSLGIISQGSVSWFVEAKPEERRKIFEDASGIGRYTKRKEEVVNQLNRTLINLKQVSVVLNELKKDLKKLTLQAEKAQQFIRVKNELKELELAVLVGEYLQAQTELDKFNFQINSSEHDFKIHEPQLELLEEQIVIFNSRFHSADMQSNELQKELQDIYQKINELEQRKVIIDVQLRQGFSQKDEKQKAAALKKLILVDQTQLDGFENQLSNSKTTITDLEKLINEQKSLVDQIKLQIEKNTADLIYQRSLKTIIELQTNELKKTNNANILVKNANALTGILNTLGTFLKFDKQYEKAILKALGKSIGYLVVNNNNAAIQAIDFLVKNEIGKVTFLPLDDVASDTKITNEHMEILKQLDGFLGVCSDHVKCDPLFQPVVNTLLAQVIIAKDLNSAINLSNYTYKLYRIVTLDGETVYAGGIINGGFEKTNLSDGYLSSASLDNEQNINKLENNERELKKELTELEVKLDEMNRKLKYEELLQAKFIERIVQIKKIILELKMEYEQLTNTTFDGKKAVASEAELIHSLNSAWAKRDEINSKLKLNQELKLQLAKTIKQSEEKIVDLRALLDEQRAKLVSAREGKIRFENTIQNITEKINSVYKMTMEFAIANHNKPVKLSSMQAHNKIAKLQNQLNEMGVINMESIAEISEKQKRFDDINAEYESAQQAVENLQKAITEIDEIASNEFDQLIQKLNQELPKTFKYLFGGGSCQIRYTDPSNVLVSGIDVFANPPGKNIANLMLLSGGEKTLVALSVLFSILKVSAFPLVILDEAESALDPANVERFANIIKTASKNTQFLIITHRQGTMMKCDMLLGAAMQTKGVTKTFAVELENAEKYVSENDSN" }, "paralog": false, "identity": 0.9968992248062015, "score": 1155.0, "evalue": 0.0, "description": "Internal stop codon at 366529, 368083. Nonsense mutation around 366529, 368083.", "psc": { "uniref90_id": "UniRef90_P47540", "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_P47540", "cog_id": "COG1196", "cog_category": "D", "kegg_orthology_id": "K03529", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47540", "UniRef:UniRef50_P47540", "COG:COG1196", "COG:D", "KEGG:K03529", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_P47540", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47540" ], "product": "Chromosome partition protein Smc" } }, "id": "KKNAAMEMLJ_678", "locus": "GCA000027325_00663" }, { "type": "cds", "sequence": "contig_1", "start": 367878, "stop": 368216, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTGTTGTGCTTTTTCAGCTTGTAATGTAAGCTTTTTTAAATCTTTTTTCAGTTCATTTAAAACAACGCTAACTTGTTTTAAGTTAATTAAGGTTCGATTTAACTGGTTAACAACTTCTTCTTTACGCTTGGTATATCTACCAATTCCCGACGCATCTTCAAAGATCTTTCTGCGTTCTTCTGGCTTAGCTTCTACAAATCAAGAGACACTCCCTTGTGAAATAATTCCAAGTGAACCTTTTTCAAGACCAATATCAGCAAAAATACCGCTAATTTCTTTTAAAGTTGCTGGATTGGAGTTAATAAAATATTCACTCTGGCCACTCCCTCTATAA", "aa": "MNCCAFSACNVSFFKSFFSSFKTTLTCFKLIKVRFNWLTTSSLRLVYLPIPDASSKIFLRSSGLASTNQETLPCEIIPSEPFSRPISAKIPLISFKVAGLELIKYSLWPLPL", "aa_hexdigest": "e6ec10abafac792283f12dc0d48fefe9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12563.762599999984, "isoelectric_point": 9.46655368804932 }, "id": "KKNAAMEMLJ_679", "locus": "GCA000027325_00664" }, { "type": "cds", "sequence": "contig_1", "start": 368081, "stop": 368530, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13175.12", "PFAM:PF13304.12", "PFAM:PF13476.12", "PFAM:PF13555.12", "PFAM:PF02463.25" ], "nt": "ATGGTTTTTCTAAAACGTTTTCGCGCATATGGTTTTAAATCATATGCTGATGAAATCACAATTGATTTTACCCATTCAATGACTGGTATTGTTGGTCCTAATGGATCGGGCAAATCCAATGTTGTTGATGCATTAAAGTGGGTTTTAGGGGAGAGAAGTATGAAACATCTCCGTAGTAAATCAGGTGATGATATGATCTTTTTTGGCTCTAAAGACAAACCTGCTAGTAAGTTAGCTGAAATAGAGCTAACCTTTGATAATTCCAACCGTTTATTACATGATTCAAGAAAAGAGATAAGCGTGATGCGTAGGGTTTATAGAGGGAGTGGCCAGAGTGAATATTTTATTAACTCCAATCCAGCAACTTTAAAAGAAATTAGCGGTATTTTTGCTGATATTGGTCTTGAAAAAGGTTCACTTGGAATTATTTCACAAGGGAGTGTCTCTTGA", "aa": "MVFLKRFRAYGFKSYADEITIDFTHSMTGIVGPNGSGKSNVVDALKWVLGERSMKHLRSKSGDDMIFFGSKDKPASKLAEIELTFDNSNRLLHDSRKEISVMRRVYRGSGQSEYFINSNPATLKEISGIFADIGLEKGSLGIISQGSVS", "aa_hexdigest": "17ea2e276da776f1adc2da1b3e150dee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13175.12", "name": "AAA_15", "length": 83, "aa_cov": 0.5503355704697986, "hmm_cov": 0.9879518072289156, "evalue": 1.1010956708625226e-11, "score": 40.01136016845703, "start": 11, "stop": 92 }, { "id": "PF13304.12", "name": "AAA_21", "length": 28, "aa_cov": 0.18791946308724833, "hmm_cov": 0.9285714285714286, "evalue": 3.255213624242689e-11, "score": 38.8541374206543, "start": 27, "stop": 54 }, { "id": "PF13476.12", "name": "AAA_23", "length": 120, "aa_cov": 0.7785234899328859, "hmm_cov": 0.9333333333333333, "evalue": 1.8514500505731484e-18, "score": 62.935909271240234, "start": 11, "stop": 126 }, { "id": "PF13555.12", "name": "AAA_29", "length": 37, "aa_cov": 0.24161073825503357, "hmm_cov": 0.972972972972973, "evalue": 2.151469939832695e-08, "score": 28.913469314575195, "start": 14, "stop": 49 }, { "id": "PF02463.25", "name": "SMC_N", "length": 137, "aa_cov": 0.912751677852349, "hmm_cov": 0.9854014598540146, "evalue": 8.588794713023003e-42, "score": 138.0916290283203, "start": 3, "stop": 138 } ], "seq_stats": { "molecular_weight": 16489.59029999998, "isoelectric_point": 9.360503196716309 }, "id": "KKNAAMEMLJ_680", "locus": "GCA000027325_00665" }, { "type": "cds", "sequence": "contig_1", "start": 368937, "stop": 369590, "strand": "-", "frame": 1, "gene": "pta", "product": "phosphate acetyltransferase", "db_xrefs": [ "COG:C", "COG:COG0280", "EC:2.3.1.8", "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959", "SO:0001217", "UniRef:UniRef50_P47541", "UniRef:UniRef90_P47541" ], "nt": "ATGCTTAATGAATCTAAGCTGATCCAACCTGCAGTTATCTTTCATAATCGTCAGGAAATCCCTGCAAATTTTGATAAAAAAATAACTCATTATGTGATTGATGAGATGGATTTAACTAGCTATGCTAACTTTGTCTATGAAAAACGTAAGCATAAGGGGATGGATTTAAAAGAAGCACAAAAGTTTGTACGTGATCCTAGTTCTTTAGCTGCTACCTTAGTTGCTCTAAAGGTTGTTGATGGTGAGGTTTGTGGTAAAGAATATGCTACAAAAGATACTTTAAGACCAGCTTTACAGTTACTAGCAACTGGTAATTTTGTTTCTAGTGTTTTCATCATGGAAAAAGGTGAAGAACGTTTGTACTTCACTGATTGTGCTTTTGCTGTTTATCCTAACTCCCAAGAGTTAGCAACAATTGCTGAAAACACCTTCAATTTTGCCAAAAGTTTAAATGAGGATGAGATAAAAATGGCTTTTTTAAGCTATTCAACGCTTGGCAGTGGTAAGGGTGAAATGGTGGATAAAGTTGTTTTAGCAACTAAACTATTTTTAGAAAAACACCCTGAATTGCATCAAAGTGTTTGTGGTGAGCTCCAGTTTGATGCTGCTTTTGTTGAAAAGGTTAGGTTACAAAAAGCACCTCAACTAACTTGA", "aa": "MLNESKLIQPAVIFHNRQEIPANFDKKITHYVIDEMDLTSYANFVYEKRKHKGMDLKEAQKFVRDPSSLAATLVALKVVDGEVCGKEYATKDTLRPALQLLATGNFVSSVFIMEKGEERLYFTDCAFAVYPNSQELATIAENTFNFAKSLNEDEIKMAFLSYSTLGSGKGEMVDKVVLATKLFLEKHPELHQSVCGELQFDAAFVEKVRLQKAPQLT", "aa_hexdigest": "e8446035e48f6b6d4d136442da255ba8", "start_type": "ATG", "rbs_motif": null, "genes": [ "pta" ], "truncated": "both", "pseudogene": { "start": 368733, "stop": 369695, "observations": { "mutation": [ 368939, 369620 ], "stop": [ 368939, 369620 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47541", "query_cov": 1.0, "subject_cov": 0.678125, "identity": 1.0, "score": 429.0, "evalue": 2.7e-150, "gene_start": 1, "gene_end": 217, "reference_start": 36, "reference_end": 252, "reference_sequence": "MSVIDIFKKRLQAVSKKPVIIFPEGWSASVLKAVEMLNESKLIQPAVIFHNRQEIPANFDKKITHYVIDEMDLTSYANFVYEKRKHKGMDLKEAQKFVRDPSSLAATLVALKVVDGEVCGKEYATKDTLRPALQLLATGNFVSSVFIMEKGEERLYFTDCAFAVYPNSQELATIAENTFNFAKSLNEDEIKMAFLSYSTLGSGKGEMVDKVVLATKLFLEKHPELHQSVCGELQFDAAFVEKVRLQKAPQLTWKNSANIYVFPNLDAGNIAYKIAQRLGGYDAIGPIVLGLSSPVNDLSRGASVSDIFNVGIITAAQAIK" }, "paralog": false, "identity": 0.99375, "score": 615.0, "evalue": 1.84e-226, "description": "Internal stop codon at 368939, 369620. Nonsense mutation around 368939, 369620.", "psc": { "uniref90_id": "UniRef90_P47541", "gene": "pta", "product": "phosphate acetyltransferase", "ec_ids": [ "2.3.1.8" ], "uniref50_id": "UniRef50_P47541", "cog_id": "COG0280", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47541", "EC:2.3.1.8", "UniRef:UniRef50_P47541", "COG:COG0280", "COG:C", "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959" ] }, "pscc": { "uniref50_id": "UniRef50_P47541", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47541" ], "product": "Phosphate acetyltransferase" } }, "id": "KKNAAMEMLJ_681", "locus": "GCA000027325_00666" }, { "type": "cds", "sequence": "contig_1", "start": 369713, "stop": 369823, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTATCTGATCAGTTTAGTTTTATCTCCACTGGTGGTGGTGCTTCTTTAGCACTAATTGGTGGGGAAGAGTTAGTAGGTATTAGCGATATTCAAAAAAATTCTTAA", "aa": "MQLSDQFSFISTGGGASLALIGGEELVGISDIQKNS", "aa_hexdigest": "72e6128bc3450b00e01137ee446d32a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3671.0473, "isoelectric_point": 4.0500284194946286 }, "id": "KKNAAMEMLJ_682", "locus": "GCA000027325_00667" }, { "type": "cds", "sequence": "contig_1", "start": 369956, "stop": 370948, "strand": "-", "frame": 2, "gene": "pgk", "product": "Phosphoglycerate kinase", "db_xrefs": [ "COG:COG0126", "COG:G", "EC:2.7.2.3", "GO:0004618", "GO:0005524", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0043531", "KEGG:K00927", "SO:0001217", "UniRef:UniRef50_Q7WTU1", "UniRef:UniRef90_P47542" ], "nt": "ATGCTTAATTTCAAAACACTCCAAGCAATTGATTTTCAAAACAAAACCGTTGTTTTAAGAAGTGATTTTAATGTCCCAATGATCAATGGGGTTATTAGTGATAGTGAAAGAATTTTAGCTGGTTTGGATACTATTAAGTTCTTAGTTAAAAAGAACTGCAAGATAGTGCTACTATCACACCTTTCAAGGATTAAGAGTTTAGAAGATAAACTAAACAACAAAAAATCTTTAAAGCCGGTTGCTGAATTACTCCAACAACTCTTACCAACTGTAAAGGTTCAATTTTCTTGTAAAAACACTGGTGCTGAAGTTAAACAAAAAGTGCAAGCATTAGCATTCGGTGAAATCCTTCTCCTTGAAAACACTCGCTATTGTGATGTAAACGATAAAGGAGAAATTGTTAAATTAGAAAGTAAAAATGATCCTGAACTAGCGAAATTCTGGGCTAGTTTAGGGGAAATTTTTGTTAATGATGCATTTGGTACTGCCCATAGAAAACATGCTTCTAATGCAGGAATTGCAAAGTATGTTGCAAAATCCTGTATTGGGTTTTTAATGGAAAAAGAACTAAAGAACCTCTCTTACCTAATTCAAAGCCCACAAAAACCCTTTGTTGTTGTTTTGGGTGGTGCGAAAGTATCAGATAAACTAAAGGTAGTTGAAAACTTACTAAAACTTGCTGATAATATCTTAATTGGCGGAGGGATGGTAAATACCTTTCTTAAAGCAAAAGGCAAAGCTACTGCTAATTCCCTAGTTGAAAAAGAGTTAATTGATGTTGCTAAGCAAATCTTGGATAAAGATACTCATAATAAGATTGTGCTGGCAATTGATCAGGTAATGGGTTCTGAATTTAAAGATCAAACTGGCATTACTTTAGATGTTAGTGACAAAATTCAAGAACAATATCAATCCTATATGTCTCTAGATGTTGGATCTAAAACAATTGCTTTATTTGAAAGTTATTTAAAAACAGCCAAAACTATCTTTTGA", "aa": "MLNFKTLQAIDFQNKTVVLRSDFNVPMINGVISDSERILAGLDTIKFLVKKNCKIVLLSHLSRIKSLEDKLNNKKSLKPVAELLQQLLPTVKVQFSCKNTGAEVKQKVQALAFGEILLLENTRYCDVNDKGEIVKLESKNDPELAKFWASLGEIFVNDAFGTAHRKHASNAGIAKYVAKSCIGFLMEKELKNLSYLIQSPQKPFVVVLGGAKVSDKLKVVENLLKLADNILIGGGMVNTFLKAKGKATANSLVEKELIDVAKQILDKDTHNKIVLAIDQVMGSEFKDQTGITLDVSDKIQEQYQSYMSLDVGSKTIALFESYLKTAKTIF", "aa_hexdigest": "faf60e5e33d37683a04722a02167dbd8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47542", "query_cov": 1.0, "subject_cov": 0.8029197080291971, "identity": 1.0, "score": 633.0, "evalue": 1.35e-227, "valid": true, "gene": "pgk", "product": "Phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_Q7WTU1", "cog_id": "COG0126", "cog_category": "G", "kegg_orthology_id": "K00927", "go_ids": [ "GO:0005829", "GO:0043531", "GO:0005524", "GO:0004618", "GO:0006094", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q7WTU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7WTU1" ], "product": "Phosphoglycerate kinase" }, "genes": [ "pgk" ], "id": "KKNAAMEMLJ_683", "locus": "GCA000027325_00668" }, { "type": "cds", "sequence": "contig_1", "start": 370950, "stop": 371963, "strand": "-", "frame": 1, "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "COG:COG0057", "COG:G", "EC:1.2.1.12", "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0006735", "GO:0009986", "GO:0016620", "GO:0044650", "GO:0050661", "GO:0051287", "GO:0140032", "RefSeq:WP_009885879.1", "SO:0001217", "UniParc:UPI000012AEC4", "UniRef:UniRef100_P47543", "UniRef:UniRef50_P47543", "UniRef:UniRef90_P47543" ], "nt": "ATGGCAGCAAAGAATAGAACCATTAAGGTTGCAATCAATGGTTTTGGAAGAATTGGAAGACTTGTTTTTCGTTCTCTTCTCAGTAAGGCAAATGTTGAAGTTGTAGCAATTAATGATTTGACCCAACCTGAAGTTTTAGCGCACCTGTTGAAATATGATTCAGCTCATGGTGAATTGAAAAGAAAGATTACTGTTAAACAAAACATCTTGCAAATTGATAGAAAAAAGGTTTATGTTTTTAGTGAAAAAGATCCCCAAAATTTACCTTGGGATGAACATGATATTGATGTAGTAATTGAATCAACTGGTAGGTTTGTAAGTGAAGAGGGTGCTTCTCTCCATTTAAAAGCAGGTGCTAAAAGAGTAATTATTTCCGCACCCGCTAAAGAAAAAACTATCAGGACAGTTGTTTACAATGTTAATCACAAAACCATTAGTAGCGATGATAAGATCATCTCAGCAGCTAGCTGTACTACTAACTGTTTAGCACCATTAGTTCATGTACTTGAAAAGAACTTTGGGATTGTTTATGGAACGATGCTAACAGTTCATGCATATACTGCAGATCAACGCTTACAAGATGCTCCTCATAATGACTTACGTCGTGCTCGTGCTGCAGCTGTTAACATTGTGCCAACAACAACAGGAGCAGCTAAAGCAATTGGGCTTGTTGTTCCAGAAGCAAATGGCAAACTTAATGGGATGTCACTCCGTGTTCCAGTGTTAACTGGTTCTATTGTAGAGTTAAGTGTTGTACTTGAAAAAAGTCCATCTGTTGAACAAGTAAATCAAGCCATGAAGCGATTTGCTTCCGCTTCTTTTAAATATTGTGAAGATCCTATTGTATCTAGCGATGTGGTAAGTTCTGAATATGGTTCAATTTTCGATTCTAAACTAACCAATATTGTTGAAGTTGATGGCATGAAACTTTATAAGGTGTATGCATGGTATGATAATGAATCTTCCTATGTACACCAACTAGTGAGAGTAGTTAGCTATTGTGCTAAGCTCTAA", "aa": "MAAKNRTIKVAINGFGRIGRLVFRSLLSKANVEVVAINDLTQPEVLAHLLKYDSAHGELKRKITVKQNILQIDRKKVYVFSEKDPQNLPWDEHDIDVVIESTGRFVSEEGASLHLKAGAKRVIISAPAKEKTIRTVVYNVNHKTISSDDKIISAASCTTNCLAPLVHVLEKNFGIVYGTMLTVHAYTADQRLQDAPHNDLRRARAAAVNIVPTTTGAAKAIGLVVPEANGKLNGMSLRVPVLTGSIVELSVVLEKSPSVEQVNQAMKRFASASFKYCEDPIVSSDVVSSEYGSIFDSKLTNIVEVDGMKLYKVYAWYDNESSYVHQLVRVVSYCAKL", "aa_hexdigest": "01db5001e72ea8a645150eda93526624", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012AEC4", "ncbi_nrp_id": "WP_009885879.1", "uniref100_id": "UniRef100_P47543", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012AEC4", "RefSeq:WP_009885879.1", "UniRef:UniRef100_P47543" ] }, "ips": { "uniref100_id": "UniRef100_P47543", "uniref90_id": "UniRef90_P47543" }, "psc": { "uniref90_id": "UniRef90_P47543", "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "ec_ids": [ "1.2.1.12" ], "uniref50_id": "UniRef50_P47543", "cog_id": "COG0057", "cog_category": "G", "go_ids": [ "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0006735", "GO:0009986", "GO:0016620", "GO:0044650", "GO:0050661", "GO:0051287", "GO:0140032" ] }, "pscc": { "uniref50_id": "UniRef50_P47543", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47543" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [ "gap" ], "id": "KKNAAMEMLJ_684", "locus": "GCA000027325_00669" }, { "type": "cds", "sequence": "contig_1", "start": 372108, "stop": 372440, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGCAGTTATATTTAAATTACTCCCAACTGTTAAAAGTGAAATTATAAGAATTAAACAAGCTCAAGCTACTAGAGGTTTTATCTATAACAAGTGTTCGTTTTTAAATCCTTTCAAGATTAAAACCCTTTTTATTCCCGTATTACTTTCAACAGTTAAAAAAACAGAAACAACAGCGTTTGCTCTACAAGCTAAAGGTTATGATTTAAATAACACTAACAGAACTCATTATCCTTTAAAATACAACTTATTAAACGGGGTATTTTTATTAGTGGGTTTACTTTTATTTAGTATTTTATTAATTGCTAATAACTGGAATTTAGTTTACTGA", "aa": "MLAVIFKLLPTVKSEIIRIKQAQATRGFIYNKCSFLNPFKIKTLFIPVLLSTVKKTETTAFALQAKGYDLNNTNRTHYPLKYNLLNGVFLLVGLLLFSILLIANNWNLVY", "aa_hexdigest": "49bee9ae139ee3bd1b583fedbdb8e75d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12565.911899999983, "isoelectric_point": 10.063337516784667 }, "id": "KKNAAMEMLJ_685", "locus": "GCA000027325_00670" }, { "type": "cds", "sequence": "contig_1", "start": 372618, "stop": 372761, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTTTCTTGTGGTTTAATGTAATTGTTAACGGTTTTATCTTTTTACCAAATACTGCTTTAAGCGTTGATCAGAACCATAATTTTTTAGGAAGTTTTATCTATTCAGGTGGTAATAATTTTGGTGGTGTTAGCTGGTGA", "aa": "MLFFLWFNVIVNGFIFLPNTALSVDQNHNFLGSFIYSGGNNFGGVSW", "aa_hexdigest": "a1e6d754f68a9901808a4d6829d752be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5302.9676, "isoelectric_point": 5.060625648498534 }, "id": "KKNAAMEMLJ_686", "locus": "GCA000027325_00671" }, { "type": "cds", "sequence": "contig_1", "start": 372926, "stop": 373999, "strand": "-", "frame": 2, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding protein MG303", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_010869419.1", "SO:0001217", "UniParc:UPI00001395D4", "UniRef:UniRef100_P47545", "UniRef:UniRef50_P75356", "UniRef:UniRef90_P47545" ], "nt": "ATGCAAACACCTCAAATAAAACTAGATTTAATTGAACAAAAATACCATCTTCAAGCTCAAAAAGCGAATCTTAAACTAGCAAAATTAGATTCAAAAAAACTGTTTTCTTTTTATAAAAAGGTTGATAAAATCATTAATCCTTTTTACTTTTTAAACAGTAAAAAAAGCATTCCATTTTTTAATAGCAAATTACTGAACATTGAACAAGATCCATTGTTTTTTAACAATATTAGTGTCTTTGTCAATAAGCATAATGATGGTCAGATCATTAAATGTTGTTCAGGGGTAATAGAACCTAATAAAATTACAGTTATTTTTGGTGAAAGTGGTAGTGGTAAAACTACACTAATAAAACAGTTAGGTTTATTTGAAAAACCTAGTTTTGGCTATATAAATTGTGCTAATTATTGTTATTTTGCAAACCAATACCAACAAAAGATAACAAAAAACTTTAAGCAAAAAATTGGTTATATTTTGCAAAAAGCAGAAGATCAGTTTTTTTGTGATTCTATTTTAGAAGAAGTTTTAACAGGAGCAGTGAACCTTAAACTTTGTCATAAAAAAGATGTTAATTATGCCAAAAAATACCTTGATCTATGTGGATTGGAAAACATCCCGTTAATAAAAAATCCCATTGAATTAAGCGATGGACAGAAGAAGCGTTTGGCATTAGCTAGTGTGTTAGCAATGCAAGTTAAGTTTTTAATCCTTGATGAACCTACTGTTGGGTTAGATCAAATGGCGATTAGCAACTTAAGCAAAATGCTAGTAGCAATGAAACAAACAACAAGGATTGTTATTGTTAGCCATGATGTTGATTTTATCTTTGAAACTGCTGATAAAATCATCCATCTCCACCAGGGAAAGATAATTCATCAAACCACTGTTAATGATTTTTTTTCAAACACCAGTTGGTTGATGCAATATGGAATCACCCCTCCAGTAATTATCCAAGCAGTTAAGATGTTTAATGAAAAGGGAATAGCTTTTAAAAACCAAGCAGAAATTAAAAACCTTGATGATCTTATTAGTGAACTAAAAAACTTATTTTCATGCAAAAAACCAGTTTTTTAA", "aa": "MQTPQIKLDLIEQKYHLQAQKANLKLAKLDSKKLFSFYKKVDKIINPFYFLNSKKSIPFFNSKLLNIEQDPLFFNNISVFVNKHNDGQIIKCCSGVIEPNKITVIFGESGSGKTTLIKQLGLFEKPSFGYINCANYCYFANQYQQKITKNFKQKIGYILQKAEDQFFCDSILEEVLTGAVNLKLCHKKDVNYAKKYLDLCGLENIPLIKNPIELSDGQKKRLALASVLAMQVKFLILDEPTVGLDQMAISNLSKMLVAMKQTTRIVIVSHDVDFIFETADKIIHLHQGKIIHQTTVNDFFSNTSWLMQYGITPPVIIQAVKMFNEKGIAFKNQAEIKNLDDLISELKNLFSCKKPVF", "aa_hexdigest": "c997a1eeb2e0f5acd2b08de7ef39d05a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001395D4", "ncbi_nrp_id": "WP_010869419.1", "uniref100_id": "UniRef100_P47545", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001395D4", "RefSeq:WP_010869419.1", "UniRef:UniRef100_P47545" ] }, "ips": { "uniref100_id": "UniRef100_P47545", "uniref90_id": "UniRef90_P47545" }, "psc": { "uniref90_id": "UniRef90_P47545", "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG303", "uniref50_id": "UniRef50_P75356", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P75356", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75356" ], "product": "Putative ABC transporter ATP-binding protein MG303 homolog" }, "genes": [ "ecfA2" ], "id": "KKNAAMEMLJ_687", "locus": "GCA000027325_00672" }, { "type": "cds", "sequence": "contig_1", "start": 373981, "stop": 374793, "strand": "-", "frame": 3, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding protein MG304", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_009885882.1", "SO:0001217", "UniParc:UPI0000557DFF", "UniRef:UniRef100_P47546", "UniRef:UniRef50_P47546", "UniRef:UniRef90_P47546" ], "nt": "TTGCTACAAGTTAAAAACCTCAGTTTTAAATACCCAAAACACCAAAACAATGTGTTAAATCAGATTAGTTTTAATGTTCAAGATGGGTGTCATTTAGCAGTAATAGGTCATAACGGTTCAGGTAAATCAACCCTAGTTAAACTCCTTGGCGGATTTCTAAAAGCTAAAAAAGGAACTATCTTTTTCAATGATAAGGAGTTGACTTCTGTTGGTTTTAATAAGATTGGAATCTTATTACAAGACCCTGATGTGCAATTACTTGCTGATACACTCCACCAGGAATTGATTTTTACTTTGGAAAACCATGGAGTTTTAGCTAGTGAAATGGATAAGATTATTAATGAAGTTTTAACTGTTGTTGAATTGAAAGATAAACAATTTACACCACTTAAAAAACTTTCATTTGGTGAAAAACAACGTGCTGTTTTTGCTTGTTTATTGGCTGTCAAACCACAAATTTATCTGCTTGATGAGGCATTTAGTATGCTTGATAATAAGATCAGCAATAAACTTAAAAAATTTGTGTTTGATACCATCAAAAAACAAAACAAAATAGTTATCAATATTACCCATGATTTTAATGATCTGTTTCTAGCAGATGAGATTATCTTTTTAAGTAAAGGATCGCTAATTAAAAAGTTTGCTCCTGAAGCGATATATGAGCAGCTTGATTTGTTTCATAACCACCACTTTAATCTCCCTTTTCCACTTTTATTGGCCCATAAGGTGGCTAAACAAATAAACAAAAAAACACCAAGTTTAAGTTTTGAATTGAATGATGTAGTTAACTGGATATGCAAACACCTCAAATAA", "aa": "MLQVKNLSFKYPKHQNNVLNQISFNVQDGCHLAVIGHNGSGKSTLVKLLGGFLKAKKGTIFFNDKELTSVGFNKIGILLQDPDVQLLADTLHQELIFTLENHGVLASEMDKIINEVLTVVELKDKQFTPLKKLSFGEKQRAVFACLLAVKPQIYLLDEAFSMLDNKISNKLKKFVFDTIKKQNKIVINITHDFNDLFLADEIIFLSKGSLIKKFAPEAIYEQLDLFHNHHFNLPFPLLLAHKVAKQINKKTPSLSFELNDVVNWICKHLK", "aa_hexdigest": "4d033138b35b5fe289f313b2d8a63aef", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557DFF", "ncbi_nrp_id": "WP_009885882.1", "uniref100_id": "UniRef100_P47546", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557DFF", "RefSeq:WP_009885882.1", "UniRef:UniRef100_P47546" ] }, "ips": { "uniref100_id": "UniRef100_P47546", "uniref90_id": "UniRef90_P47546" }, "psc": { "uniref90_id": "UniRef90_P47546", "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG304", "uniref50_id": "UniRef50_P47546", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P47546", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47546" ], "product": "Putative ABC transporter ATP-binding protein MG304" }, "genes": [ "ecfA2" ], "id": "KKNAAMEMLJ_688", "locus": "GCA000027325_00673" }, { "type": "cds", "sequence": "contig_1", "start": 374979, "stop": 376706, "strand": "-", "frame": 1, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_P9WMJ9", "UniRef:UniRef90_P47547" ], "nt": "ATGAGTGCAGACAATGGTTTAATTATTGGCATTGACCTTGGAACTACCAATTCTTGTGTTTCTGTAATGGAAGGTGGGAGACCTGTTGTATTAGAAAATCCTGAAGGTAAAAGAACAACACCTTCCATTGTTTCCTATAAAAACAATGAAATTATAGTAGGTGATGCTGCTAAAAGACAGATGGTTACAAACCCAAATACCATTGTCTCCATCAAGAGGTTGATGGGTACCTCAAATAAAGTAAAAGTCCAAAATGCTGATGGTACAACTAAGGAATTAAGTCCTGAACAAGTTTCAGCGCAAATCCTTAGTTATCTTAAGGACTTTGCTGAAAAAAAGATTGGTAAAAAGATTTCAAGAGCAGTTATTACTGTTCCTGCATACTTTAATGATGCGGAAAGAAACGCTACTAAAACCGCAGGTAAGATTGCTGGTTTAAATGTTGAAAGGATCATTAACGAACCAACTGCTGCTGCTTTAGCTTATGGGATTGATAAAGCATCAAGAGAGATGAAAGTCTTGGTTTATGACTTGGGTGGTGGAACTTTTGATGTATCTTTACTTGACATTGCAGAAGGTACTTTTGAAGTACTTGCAACTGCTGGGGACAACCGTTTGGGAGGTGATGATTGGGATAACAAGATCATTGAATATATCTCAGCCTACATTGCCAAAGAACACCAGGGTTTAAACTTATCAAAAGATAAGATGGCAATGCAACGGCTTAAAGAAGCAGCTGAACGTGCTAAGATTGAACTTTCCGCTCAACTTGAAACGATTATTTCTCTACCATTTTTAACTGTTACCCAAAAAGGTCCTGTTAACGTTGAGTTAAAACTAACCCGTGCTAAGTTTGAGGAGTTAACAAAACCACTACTTGAAAGAACAAGAAACCCTATTTCAGATGTTATCAAGGAAGCTAAGATTAAACCTGAAGAGATTAATGAAATTCTTTTAGTTGGTGGTTCTACAAGGATGCCTGCAGTTCAAAAGCTAGTTGAATCAATGGTACCAGGTAAAAAACCAAACCGTTCTATTAATCCTGATGAAGTTGTTGCTATTGGCGCTGCTATTCAAGGTGGGGTTTTACGTGGTGATGTTAAGGATGTTTTACTTTTAGATGTAACTCCATTAACCCTTTCTATTGAAACTTTAGGTGGTGTGGCTACTCCTTTAATTAAGAGAAATACAACTATCCCAGTAAGTAAAAGTCAAATCTTTTCAACTGCTCAAGATAACCAAGAATCAGTTGATGTGGTTGTATGTCAAGGGGAAAGACCAATGTCTAGAGATAATAAGTCATTAGGAAGATTTAACTTAGGTGGTATTCAACCAGCACCTAAAGGTAAACCCCAAATTGAGATTACCTTTAGTTTGGATGCCAATGGGATCTTAAATGTTAAAGCTAAGGATTTAACCACGCAAAAGGAAAACAGTATTACCATTAGTGACAACGGTAATCTTTCTGAGGAGGAGATCCAAAAGATGATCCGTGATGCTGAAGCTAACAAGGAACGGGATAACATCATCCGTGAACGTATTGAATTACGTAATGAAGGGGAAGGTATTGTTAATACCATCAAAGAGATATTAGCAAGTCCTGATGCTAAGAATTTCCCTAAAGAAGAAAAAGAGAAGTTAGAAAAGCTAACAGGTAACATTGATGCTGCTATTAAAGCTAATGACTATGCCAAACTCAAAGTGGAAATTGAAAACTTTAAGAAGTGA", "aa": "MSADNGLIIGIDLGTTNSCVSVMEGGRPVVLENPEGKRTTPSIVSYKNNEIIVGDAAKRQMVTNPNTIVSIKRLMGTSNKVKVQNADGTTKELSPEQVSAQILSYLKDFAEKKIGKKISRAVITVPAYFNDAERNATKTAGKIAGLNVERIINEPTAAALAYGIDKASREMKVLVYDLGGGTFDVSLLDIAEGTFEVLATAGDNRLGGDDWDNKIIEYISAYIAKEHQGLNLSKDKMAMQRLKEAAERAKIELSAQLETIISLPFLTVTQKGPVNVELKLTRAKFEELTKPLLERTRNPISDVIKEAKIKPEEINEILLVGGSTRMPAVQKLVESMVPGKKPNRSINPDEVVAIGAAIQGGVLRGDVKDVLLLDVTPLTLSIETLGGVATPLIKRNTTIPVSKSQIFSTAQDNQESVDVVVCQGERPMSRDNKSLGRFNLGGIQPAPKGKPQIEITFSLDANGILNVKAKDLTTQKENSITISDNGNLSEEEIQKMIRDAEANKERDNIIRERIELRNEGEGIVNTIKEILASPDAKNFPKEEKEKLEKLTGNIDAAIKANDYAKLKVEIENFKK", "aa_hexdigest": "b49bf5cdab9e5602a3ee02aa698930a5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47547", "query_cov": 1.0, "subject_cov": 0.9663865546218487, "identity": 1.0, "score": 1083.0, "evalue": 0.0, "valid": true, "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_P9WMJ9", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_P9WMJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P9WMJ9" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "KKNAAMEMLJ_689", "locus": "GCA000027325_00674" }, { "type": "cds", "sequence": "contig_1", "start": 376944, "stop": 377207, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTAGCAATTGTTTCCTACTTTAGTTTGTTTGGTGTTTTTATGATCACAAAAACTGTCAACACAACTAATAACGTTATTGATCAATACAGCTTAATTGATCTTTTTAACATTACTAATATTGCTGTTACCGCTTCTAACTTTATTCCAGTTGCTAGTGGGGAAGGTGCAACACAATTTGTGATGACTAGTTTTCTCAATGCATTCAAACCAACTGATCAATTCCTCCATGATCAGATTAAAGATGGTGTTTTTTTATGA", "aa": "MVVAIVSYFSLFGVFMITKTVNTTNNVIDQYSLIDLFNITNIAVTASNFIPVASGEGATQFVMTSFLNAFKPTDQFLHDQIKDGVFL", "aa_hexdigest": "0e818fcc072ad2cf1ab783cceb27ea3d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9603.948199999993, "isoelectric_point": 4.451254844665528 }, "id": "KKNAAMEMLJ_690", "locus": "GCA000027325_00675" }, { "type": "cds", "sequence": "contig_1", "start": 377703, "stop": 377975, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGTTAACCACCAATACCTTTTTTAACACTAAAAACATCGTTGCTTTTAGCTTTTTTTTGGTTTTTCTAATCGTTATTAGTGTTATTGTTACTATCTTTTTTTTAGGTGTTAGTGTTGATGATGTGAAAACTATTATCACTGCAATTAACTATCAGAATTGGGGTTGGATATTTGTTGTTATCTTAGGGTTTTTAGTTTCAGTTTTGTGGAATGTAATCATTAATTGGTGGGTATCAAGGAGATTTTGTTTTTATGCTAGTTGGTGA", "aa": "MAKLTTNTFFNTKNIVAFSFFLVFLIVISVIVTIFFLGVSVDDVKTIITAINYQNWGWIFVVILGFLVSVLWNVIINWWVSRRFCFYASW", "aa_hexdigest": "da1c4f127275045829134d49d3f9934c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10539.441099999998, "isoelectric_point": 9.514002418518068 }, "id": "KKNAAMEMLJ_691", "locus": "GCA000027325_00676" }, { "type": "cds", "sequence": "contig_1", "start": 377975, "stop": 379027, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGGTAATCCTAATAATGGCAATATTGATGAATTGTTTAAAAAAGCTTTTTTAGAGAGTGTTGATGAATCAGGTTTTAATGTTAACTTTAAAGCAGTTATTGACCATTATCGCTTCATTTTTACTTTGCAATGGTTAATTGAAAACAACCTAAAAAACTTTAAAGATATTTTACAAGCTAACTTAAAGTATGGTGAAATTGCCTATATTGCTTATGATAAGAATATTATCAGTAACAACACCAATAATCCTCAGGGAATATTTGGCTCTGTTTTTAACTATGAAAATGATGAACATGCTACAACAGCTAATGCCAACCAAACTATTGATCCTAATAATTTCTTTTTTAAAACCAAAACAAGTCCTAACACTACACCTGCTACTACGATGTTAAGCACCAGACAAGCAGTATCAACAAGTAATAATGGTACTTATGGTTTTACTGGTTTGAATACAACTAACAGTTCCATGTTAGAAAACAATACTAACCAGGCACTTTTAAACCATATCGCTGCCAATTCCTTAAAACAATATGGTTCAAAGGATGATCTTAAAAAATTTATTAGTGAAACAAAAGACCAATTAGTTTTAGATAACATTGCCCGACAGCTAAGTAGACTAACTCCAAGTAGTAGTAGTAGTAATGGCAAAACACTTTCAGCTTATTTTCAGGTCGATGCAATTAACAATTCTGCTTTAGATTTCAAAGCTAAACAAGCGTTGTTATTAGCTGTTTTAGACCAATATTCCAGTTATTTTAATTCATCAAATAGTCCAAGTATTTCAAAAAGAAGTACCCAAACCAACCAAAAATTTAGTGAATTTAACTTTGGTGGTGATAGTTACAACTATCTTCAGTTCACTAAAAGTGACATTGATTCACTTAGTTTAACAAGTTCTACCAATATTGAAAGTGATATAGTAGCAGCTTTAGTTTTATTTCAAGCTAGTGATACAGGCACCCAACAACTTGCTTTAAGTGCAATTGAAAAACCCCAATTTAGGATTGGTGATAAAAGAATTCAATCAGGCTTAAACTTGCTTAAGTAA", "aa": "MYGNPNNGNIDELFKKAFLESVDESGFNVNFKAVIDHYRFIFTLQWLIENNLKNFKDILQANLKYGEIAYIAYDKNIISNNTNNPQGIFGSVFNYENDEHATTANANQTIDPNNFFFKTKTSPNTTPATTMLSTRQAVSTSNNGTYGFTGLNTTNSSMLENNTNQALLNHIAANSLKQYGSKDDLKKFISETKDQLVLDNIARQLSRLTPSSSSSNGKTLSAYFQVDAINNSALDFKAKQALLLAVLDQYSSYFNSSNSPSISKRSTQTNQKFSEFNFGGDSYNYLQFTKSDIDSLSLTSSTNIESDIVAALVLFQASDTGTQQLALSAIEKPQFRIGDKRIQSGLNLLK", "aa_hexdigest": "45e598f59d95c5711f9933ff31388b56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38871.57880000007, "isoelectric_point": 5.976699638366699 }, "id": "KKNAAMEMLJ_692", "locus": "GCA000027325_00677" }, { "type": "cds", "sequence": "contig_1", "start": 379049, "stop": 379756, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGCATTCAATGATTACAAAGAACTAATGCAAAAATTTGCACCAGTTGCATTAGCAACAAATGTAATCAGAGATTTGTTTTTACAAATGGATGCATTAGATAACAAACTAACAAGTAGAACAACTGAACTTAATACCAATGTTAACCAAATTAATCCAACCCCTTGGTTAAATGGCTTATCTTCCCATTTGCCATATGTTCGCAAGTCTGGTCATTATGAAAAACTCAATAACTATTTCTTGTTTTTAATTACTAAAACACTTTGGAAAAAAGTAGGTAGTGAAAAAATAAATATTAGTGAAAACAACAATAAGTTAAAAACTACAAAAGCTGATGTTGATAAGATAAGAGATGAAATTCTGAGCGACATAAATAACAAAGTAACAGAATTTGTTAACCAATTGAAAGTTACAGAAAAATCAAAACCTAACTTTAGCAACATTATTCTAGTTGATATTAACAATGATCAATCACTTACTAACAGTGCCAATTGGAGTTTAAATGCGTTGTTAGATGTAACAACTGTAAACCCTCTTTCCTTTGCATTGTTAAAAAATGCTTTTACTAGCAATCAACAGTTTGAAAAAGCTAAAAAGTTATTTGAAGAGATTAAATCTAAAAATGGATCTTCATCAACTTCATCAAGTAGTGATGCAGATAGTTTAGCTAAAGTAATTAGCAATTACTATTACATGACATGA", "aa": "MQKAFNDYKELMQKFAPVALATNVIRDLFLQMDALDNKLTSRTTELNTNVNQINPTPWLNGLSSHLPYVRKSGHYEKLNNYFLFLITKTLWKKVGSEKINISENNNKLKTTKADVDKIRDEILSDINNKVTEFVNQLKVTEKSKPNFSNIILVDINNDQSLTNSANWSLNALLDVTTVNPLSFALLKNAFTSNQQFEKAKKLFEEIKSKNGSSSTSSSSDADSLAKVISNYYYMT", "aa_hexdigest": "675dc3db95f98a847ed45261eceb5c4f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26675.922800000026, "isoelectric_point": 9.155235862731931 }, "id": "KKNAAMEMLJ_693", "locus": "GCA000027325_00678" }, { "type": "cds", "sequence": "contig_1", "start": 379922, "stop": 380353, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCTGTGAACACAATACAACAGACAGTTCACAAAACAATTTAAAGAGTCTTATTAAAGATGCTTTTATAAGTTCTAATGATAGTTCCAATTTAGGAAAACTAGCAAAGCAAATCCACCAAACAACTACAAGTGATATGATGGTTTCAACTAAAGCTAGTTCATCAACAACAAGCAGTTATCTTGTTTGGGATGCAGCTATTCCTAATAACAAAACAAATAATGGTGCTAGCACTGTTAGTGCAAACTGTGCAAATGCTACTGTTCAAAATACAAGCCATAACTCTAATAACCAATTGAAGCTAAGACTAGTTAGAAACGGAGAGGGAGTTGCTGTAATTGGAATTGATGGAGGTAGTTATTATCTGACAAAAAATAGTAGTAGCAAAACTGAAAGAGACATTGAAAAGCAAAAGCAGTTTTTAATGTGA", "aa": "MFCEHNTTDSSQNNLKSLIKDAFISSNDSSNLGKLAKQIHQTTTSDMMVSTKASSSTTSSYLVWDAAIPNNKTNNGASTVSANCANATVQNTSHNSNNQLKLRLVRNGEGVAVIGIDGGSYYLTKNSSSKTERDIEKQKQFLM", "aa_hexdigest": "13139f6c43c45cbaa3b7401ad532b5c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15470.92739999998, "isoelectric_point": 8.928565025329593 }, "id": "KKNAAMEMLJ_694", "locus": "GCA000027325_00679" }, { "type": "cds", "sequence": "contig_1", "start": 380675, "stop": 380899, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCAAAGTTACAAGCACAAATCTTTAATCTCTGGGTTGAATATACCGATCCTACTTTAATCAGTCAAGCTACTTTTAAATATGCAGCACCACAACAAGGTTTGGGTCAAGTTTACAACACTGAAAAACTAAAAGATAAATTAACCCCTTCTTATGCGTTTCCTTTCTTTCCTAAAGATGGGGAAATTCCTCAGAACCAGAATGTTGGCAACAAACGCTGA", "aa": "MFAKLQAQIFNLWVEYTDPTLISQATFKYAAPQQGLGQVYNTEKLKDKLTPSYAFPFFPKDGEIPQNQNVGNKR", "aa_hexdigest": "e9a883727d7d1cffa9a817c3bf33fddb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8485.5487, "isoelectric_point": 9.004250907897951 }, "id": "KKNAAMEMLJ_695", "locus": "GCA000027325_00680" }, { "type": "cds", "sequence": "contig_1", "start": 381089, "stop": 381508, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_442", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "nt": "ATGAAAAAACTTTTAAAAAAATCAAAGTTCTGGTGGTTTTTACTTTGTGGTTTGTCAGTAAGCACAATACTTGTAGCTTGTTCAACTCCAACAAACAGTGCTTTGCAAACAGTTTTTTCACCAACCTCAACTAATTTTTTTCATGGTCAAAAGGGTAGTTTAAAAGAAGGACTTATTACTGCTTTAAAAACTCCTTCAGCAAACAAACATTTTGTTATAGGACCACTTTTAAAAGCACTTGAGGCTTGGTATGAAAACAATGAAGACAAAGCCATAAACAAGTTTTTAAAAGACACTAAAAAGAATGTTGATGATCAATATAAAAATACTGTTAATGGGTTAATATCACCACCCAGAAACAGAGCAGTATTTATTCAACAAGATCTATTAGATAAAAGTGGTGGCAGTGAGGCTAGTTGA", "aa": "MKKLLKKSKFWWFLLCGLSVSTILVACSTPTNSALQTVFSPTSTNFFHGQKGSLKEGLITALKTPSANKHFVIGPLLKALEAWYENNEDKAINKFLKDTKKNVDDQYKNTVNGLISPPRNRAVFIQQDLLDKSGGSEAS", "aa_hexdigest": "10e6f857f9c6bac6b02964ccfe5aeb4d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75336", "query_cov": 0.9784172661870504, "subject_cov": 0.9006622516556292, "identity": 0.603, "score": 159.0, "evalue": 9.37e-48, "valid": false, "product": "Uncharacterized lipoprotein MPN_442", "uniref50_id": "UniRef50_P75336", "go_ids": [ "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "product": "Uncharacterized lipoprotein MPN_442" }, "genes": [], "id": "KKNAAMEMLJ_696", "locus": "GCA000027325_00681" }, { "type": "cds", "sequence": "contig_1", "start": 381505, "stop": 381681, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGTTTTTGACTGAAAATAACCAAAAAACTACCAAAAAACCGCTTTCAACTTTACAAATTAAGAAAATTAAAGCTATCAAAGCTAAATATAAAAAGGTTAAACCTAACTACAAAAAATACCAAAAACAACAGATTCAAAACCTTTTAATTAATAAGAAAAAACCAAGATCATGA", "aa": "MKFLTENNQKTTKKPLSTLQIKKIKAIKAKYKKVKPNYKKYQKQQIQNLLINKKKPRS", "aa_hexdigest": "db354a7ab134bfb140c51409a315f68c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6960.372600000004, "isoelectric_point": 10.623052024841307 }, "id": "KKNAAMEMLJ_697", "locus": "GCA000027325_00682" }, { "type": "cds", "sequence": "contig_1", "start": 381853, "stop": 382269, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37" ], "nt": "TTGTTTGCCTTTTTTAGTGCTACTTTATTCAATCAACAACTCCAAATAATTAAAAAACAAGTAATTAATACTAAAGTTATTAATCTCCATCCAAAGCAATGGATTCACCCCTTAGTTAAGCATTTTGTTGTTCATCTCAATACTGAAAACCGTTTTTCTGGTTTATTAGCCTTATTAAAACACCACCAAAATCAGCAAATTATTGTTTTCTGTTCTAATCAAAAATCTTTAAAACAATTAACACAATTGCTTTCAAATAACAATATTAGTTTTGGTTCAATTTATGGTAGTTTAACCTATCAAGAACGTAAAAATAATTTCACAAAAGCAACTAATAATAAATTGAAATTACTAGTTGTTTCTGATCTATTTTCACGTGGGATAGATTTAAATTATTTCAGTGTGGTGATTTCTTGA", "aa": "MFAFFSATLFNQQLQIIKKQVINTKVINLHPKQWIHPLVKHFVVHLNTENRFSGLLALLKHHQNQQIIVFCSNQKSLKQLTQLLSNNNISFGSIYGSLTYQERKNNFTKATNNKLKLLVVSDLFSRGIDLNYFSVVIS", "aa_hexdigest": "7e2a69c49f7eff7cbea2f7755ce40229", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 85, "aa_cov": 0.6159420289855072, "hmm_cov": 1.0, "evalue": 1.2428145570684257e-17, "score": 59.229312896728516, "start": 53, "stop": 137 } ], "seq_stats": { "molecular_weight": 15959.388299999986, "isoelectric_point": 10.178349113464353 }, "id": "KKNAAMEMLJ_698", "locus": "GCA000027325_00683" }, { "type": "cds", "sequence": "contig_1", "start": 382747, "stop": 383556, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAGTGCACAGCAAACCAATAACCAGCAAAGAAGGTTTGGTTTCCATGGTATTGTTACCAACACTTCATCCAACAACTTACCAGATGCAGTTAGAAATCGCCTCTTTACAAGTTTTGTTTCCCAATCTGAAAAATCAAGTTCTAATGGTGGTCAAGCACAATTACAATCTACTCAATCAAGTGGTAGTAATGAAACAATTTACAAAGGTGCTTTATTTAGTTTTGGTTCTCTAACAAAACTTATAGAAACAATTGATAACATCCCAACCCAAGCTGAGTTTGATGCACTTTATAACCATTTAACAAGTAACCTTAATATTAACGTTACTGGTGTTGATAGATCAAAATCCTTACAAGAACAAAAAACTAATTTGAAGAATTTTGCTAATTCAAACTTTAACAATACTCAAACTGTTCAATTAAAACAAGCTCAAAGTAAAACTAATAATAGTAACTTTAATGATGTGTTTAGTAGGTTTGAAGGGTATATAGGTACAAATAAAACCAGTAATTATAGCAGTTATAACTTTTTACAAGATAACCAGATCTATCATGCAGTTTATGCAAAACAGATTAACCTTGAAGATGTAAGTATGCTTGGTAGTGATAGTTTGAATAGTACAGATTCAAATAATTCAAAAAGACTTGATCTTTCACTTGAAGAATTTTTAAGTACAGTTGCGCTTGAAGCGCTTAATCCTAATAATCAAACCCAAGCTATTAATGCATTGATAGCAAATGCCAAAAATGGCTTAGTTAGAGTTGGTGATAACAGATTGTTTTCAGCAATCAGTTCACAGTGA", "aa": "MQKSAQQTNNQQRRFGFHGIVTNTSSNNLPDAVRNRLFTSFVSQSEKSSSNGGQAQLQSTQSSGSNETIYKGALFSFGSLTKLIETIDNIPTQAEFDALYNHLTSNLNINVTGVDRSKSLQEQKTNLKNFANSNFNNTQTVQLKQAQSKTNNSNFNDVFSRFEGYIGTNKTSNYSSYNFLQDNQIYHAVYAKQINLEDVSMLGSDSLNSTDSNNSKRLDLSLEEFLSTVALEALNPNNQTQAINALIANAKNGLVRVGDNRLFSAISSQ", "aa_hexdigest": "5210017a7e8bff392c49153ed5b0fd3c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29728.128400000034, "isoelectric_point": 7.891591072082519 }, "id": "KKNAAMEMLJ_699", "locus": "GCA000027325_00684" }, { "type": "cds", "sequence": "contig_1", "start": 383656, "stop": 383787, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGTAAGTTCTTCTAAATCTGTATTAGTAAAAATGGCTAACAACGTTGCTTCTCAAACTAATCAAGACTTTACCAAGCAAGAACAACAAAATCCTAACTATGTATTTGGTTCGAGTGCTTATAATTGA", "aa": "MSVSSSKSVLVKMANNVASQTNQDFTKQEQQNPNYVFGSSAYN", "aa_hexdigest": "434ac94a7d5d89db34df5fe6aa1e22bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4730.1202, "isoelectric_point": 8.1392786026001 }, "id": "KKNAAMEMLJ_700", "locus": "GCA000027325_00685" }, { "type": "cds", "sequence": "contig_1", "start": 384148, "stop": 385464, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAAGCTAGTGATATGTTTGATACCTTTGATCCTAGCTTTTCAGCAGCTTTTATCCAGGGCTATTTAGCTTTACAAAAAAAATCCAAAGGTGCAGAACAAACTGAATACACTAAATTAGAAAAAGATAAATCAATCATTGAAAATTTCTTTGTTGAAAATAATTCAGCTAAGGCTGCAATGAAAAGTGCAAGTAGTAGTTCACAAACAACTACTGTTCATAAAACAGATCTAGCTAAGATCTTTAAAGAAAATGAAACTACTAAATCAACAGATGTTTTTAAAGGTGAATATCAAAAAAAGTTCAGTAATGCTACTAGTACAAGTAACAGTGATAGTAGTAATAATTCTGCAATCGTTGATCTAAAAGAACTTAAAAAAGATAACAATAGTCAACCTGATTTAATCTTAGCACGTGGTAAAGATGGTATCCATCTAATGGGTGTTGATGGTGGTGGTTATTATCTCTCTGAATCAGGACGTGATGTAAATAAACAAAAACAATTCTTATTATTCCGTGCACTACAAACCAAATATGGCTTAATTGATACAAACACTACTTATGACTTTAAGTTGTTTGATGAAGTGAAAAAACACTTTGATAAGAACAGAGTTTTATTTTTATTTAATGCCCTTTTTAAATTAGTAGACAGCAAAGAAAGTAACTTTTTAAGTTTCCCTCAGTTTAAAAAGTTTAGTGATAGCATTATCACTGTTAAAAACGAGTTGAAGGATTTAGTTGAAAGTCAATATCAACAAATTGTTTTTAATGAAGTTGCTACTGCAGAAAACAAAGTTGCTTTAAAACTAGCAGAACGTAACCAACCTTTCATTGATAATGAGCGAAATAAACAAATATGGATGAACGGTTTAGCTGCTGTTTTACCTTATGAACAAGATAGTAAAACAGGACACTACAATGAACTTGGTATTTACTATAAAGACATCATTGATAAAGTTAGTTCAAATACAAGTAATAGTAATAGTAATAGTAATAGTAGTAGTAGTGATCCTTTTTCAAAAAAGATAATTGACAAGTTAAAAGAGAATAAAAAGAAAGTTGAAGCAGCAGTTAAAAAGCATGTTGATGAACTAAAAGTTTCGGTAATTCCCTCACCGCAATATTCACAAATTATCCTTGTTGATACCAAACTTTCATCTGATCCTAGAAACACTTCTCTAGCACTTAATTTAGCTTTAAATGCTGTTTTATCAAGTGATGAGTTACAAAATACCATTAGACGAGATTATTTTGTCAATGATGATCAGTTTAAACAAGCGATTGATTTAGATAAATTAACTTTTAAAAACTGA", "aa": "MLKASDMFDTFDPSFSAAFIQGYLALQKKSKGAEQTEYTKLEKDKSIIENFFVENNSAKAAMKSASSSSQTTTVHKTDLAKIFKENETTKSTDVFKGEYQKKFSNATSTSNSDSSNNSAIVDLKELKKDNNSQPDLILARGKDGIHLMGVDGGGYYLSESGRDVNKQKQFLLFRALQTKYGLIDTNTTYDFKLFDEVKKHFDKNRVLFLFNALFKLVDSKESNFLSFPQFKKFSDSIITVKNELKDLVESQYQQIVFNEVATAENKVALKLAERNQPFIDNERNKQIWMNGLAAVLPYEQDSKTGHYNELGIYYKDIIDKVSSNTSNSNSNSNSSSSDPFSKKIIDKLKENKKKVEAAVKKHVDELKVSVIPSPQYSQIILVDTKLSSDPRNTSLALNLALNAVLSSDELQNTIRRDYFVNDDQFKQAIDLDKLTFKN", "aa_hexdigest": "e7e00997f54a50ead8d402586c071917", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 49520.05660000003, "isoelectric_point": 8.498367500305175 }, "id": "KKNAAMEMLJ_701", "locus": "GCA000027325_00686" }, { "type": "cds", "sequence": "contig_1", "start": 385987, "stop": 386349, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAATTGTTGCTGTTGCCTGTGCACAACCAAACTCCAGAACCATTGAAAATCTCTTTAGACCCAGTAGTGCTTTTACTGATAAAAATGATGGTAGTATTAATGCCACACTTTATAAAGCACTTGAAAATAGAGAAGGACTAACGCAATATTTAACGATGCGCCTAGCGCCAGTATTGCGCAATTTTTATGAAGAAAATGTTGATGATGACATTAAGAGAAATTTAAGAACTTTTAATACTGATACAGACAATAGTTTTGTTAATCAAGAACAAAACTTAAGAAATCAGTATCGGGGTGATTATTTAGTTAGGTTACAAACTGATATTTTAGATAACACTGGTGGAAATCAAGCCAACTGA", "aa": "MGIVAVACAQPNSRTIENLFRPSSAFTDKNDGSINATLYKALENREGLTQYLTMRLAPVLRNFYEENVDDDIKRNLRTFNTDTDNSFVNQEQNLRNQYRGDYLVRLQTDILDNTGGNQAN", "aa_hexdigest": "f6b597b7100c4a3218f8b2035cabfd0a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13696.93039999998, "isoelectric_point": 4.871010780334472 }, "id": "KKNAAMEMLJ_702", "locus": "GCA000027325_00687" }, { "type": "cds", "sequence": "contig_1", "start": 386463, "stop": 386678, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGACTTGAAATAGAAAACGGGCTTGAATTTGTCAATGATCCTGTGGTAAATGAACTTGGCAAGATCTGTTTTTTTCATCCTTTTACAGGTAATTTAACAAACAAACTTAGTTTCAGAAGTCATTTCAATAGATACAGTTTTTATGCCATTAACTACCCAGGGCATGGTAATAGTGTTATTAACAATCCAAAGCAACTTGAATTTAGTTACTGA", "aa": "MRLEIENGLEFVNDPVVNELGKICFFHPFTGNLTNKLSFRSHFNRYSFYAINYPGHGNSVINNPKQLEFSY", "aa_hexdigest": "0559d41656c46e83637a9a9b1e1bb444", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8287.231399999997, "isoelectric_point": 6.815188407897949 }, "id": "KKNAAMEMLJ_703", "locus": "GCA000027325_00688" }, { "type": "cds", "sequence": "contig_1", "start": 386679, "stop": 387092, "strand": "+", "frame": 3, "gene": "menH", "product": "Putative esterase/lipase 1", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0016020", "GO:0016298", "SO:0001217", "UniRef:UniRef50_P75333", "UniRef:UniRef90_Q49412" ], "nt": "TTGGAAATTACAAAACAGTTTTTTGATAAACATAACTTAAAGGATGTAATCTTATTTGGTCATTCTATTGGCGGTGGTTTAGCAGTTGCTTTAACTAACTATTTAAGCAGTGATCAATATAAAGCAGTCTTGTTAGAAGCACCATTAAATCCTGCTATTGTTGAAACTCCTTTAAATATTGTTCAAAATCTCATTCCTGATCCTGATAGTGATTTTGCTGTTATTCAAAAGTGTCTTGTTTACAATATTGAGAAAAAACTTGGGGCAAACTTTAAAGAATATTGTGAAAGAGAAAAGCAAAAATCAATTCACCAAAACCAACGCCTTAAAGTGATGTTAGAACCTTCTACACTCAAACAAAACATAGTTTTAATCAATGCAGCTTTTTTAAAGTTAAATTGTCCTGCTTTGTGA", "aa": "MEITKQFFDKHNLKDVILFGHSIGGGLAVALTNYLSSDQYKAVLLEAPLNPAIVETPLNIVQNLIPDPDSDFAVIQKCLVYNIEKKLGANFKEYCEREKQKSIHQNQRLKVMLEPSTLKQNIVLINAAFLKLNCPAL", "aa_hexdigest": "117a70ad617aa154477b429457924bb0", "start_type": "TTG", "rbs_motif": null, "genes": [ "menH" ], "truncated": "both", "pseudogene": { "start": 386463, "stop": 387269, "observations": { "mutation": [ 386676, 387090 ], "stop": [ 386676, 387090 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49412", "query_cov": 1.0, "subject_cov": 0.5111940298507462, "identity": 0.993, "score": 266.0, "evalue": 2.03e-88, "gene_start": 1, "gene_end": 137, "reference_start": 73, "reference_end": 209, "reference_sequence": "MRLEIENGLEFVNDPVVNELGKICFFHPFTGNLTNKLSFRSHFNRYSFYAINYPGHGNSVINNPKQLEFSYWLEITKQFFDKHNLKDVILFGHSIGGGLAVALTNYLSSDQYKAVLLEAPLNPAIVETPLNIVQNLIPDPDSDFAVIQKCLVYNIEKKLGANFKEYCEREKQKSIHQNQRLKVMLEPSTLKQNIVLINAAFLKLNCPALWIHGKQDGIIKYLPSKAYYESLNNKQIQFKAIEAAAHTPYFEQPQKFLSLVNDFFQLIS" }, "paralog": false, "identity": 0.9925373134328358, "score": 536.0, "evalue": 2.8e-197, "description": "Internal stop codon at 386676, 387090. Nonsense mutation around 386676, 387090.", "psc": { "uniref90_id": "UniRef90_Q49412", "gene": "MenH", "product": "Putative esterase/lipase 1", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75333", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0016020", "GO:0016298" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49412", "EC:3.1.-.-", "UniRef:UniRef50_P75333", "COG:COG0596", "COG:HR", "GO:0016020", "GO:0016298" ] }, "pscc": { "uniref50_id": "UniRef50_P75333", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75333" ], "product": "Putative esterase/lipase 1" } }, "id": "KKNAAMEMLJ_704", "locus": "GCA000027325_00689" }, { "type": "cds", "sequence": "contig_1", "start": 387297, "stop": 387893, "strand": "-", "frame": 1, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274", "KEGG:K02986", "SO:0001217", "UniRef:UniRef50_P46775", "UniRef:UniRef90_P47553" ], "nt": "ATGAAATATACTGGTAGTATTTTCAAACGATCAAGACGTTTAGGTTTTTCTTTACTTGAAAACAACAAAGAGTTTTCCAAAGGAAAAAAACGTAAATCTATTCCCGGTCAACATGGAAATAGGTTTCGTTCCTCAACTTTATCAGGTTATGCCCAACAACTCCAAGAAAAACAAAGGATGCAATACATGTATGGTATTACTGATAAACAATTTCGTAGGTTATTTCGCTTTGTTTTAAAACAAAAAGGTAACTTAACAGTTAATTTATTTAGAGTTTTAGAATCACGTTTGGATAACATAGTTTACAGAATGGGTTTTGCACCAACAAGAAAGAGTGCAAGACAAATGGTAAACCACGGTCATGTGATTTTAAATGATCAAACTGTTGACACCCCTTCAATCATCATTAATCCCGGGGATAAAGTCCGTTTAAAAGCAAGAATAACTAAATCCCCATTAGTTAAAAATTTTATTGAAAACAGTGTTATCTCATCATTTGTGGAAACCAACAAAAAGGCATTTGAAGGTACTTATATAAGATTCCCAGAGCGTAGCGAACTACCTGCTGGAATAAATGAATCTTACGTTGTTGAGTGA", "aa": "MKYTGSIFKRSRRLGFSLLENNKEFSKGKKRKSIPGQHGNRFRSSTLSGYAQQLQEKQRMQYMYGITDKQFRRLFRFVLKQKGNLTVNLFRVLESRLDNIVYRMGFAPTRKSARQMVNHGHVILNDQTVDTPSIIINPGDKVRLKARITKSPLVKNFIENSVISSFVETNKKAFEGTYIRFPERSELPAGINESYVVE", "aa_hexdigest": "e0a00388f78d267ac1d8e235e27a2c68", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47553", "query_cov": 1.0, "subject_cov": 0.9658536585365853, "identity": 1.0, "score": 384.0, "evalue": 6.92e-135, "valid": true, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_P46775", "cog_id": "COG0522", "cog_category": "J", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274" ] }, "pscc": { "uniref50_id": "UniRef50_P46775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P46775" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "KKNAAMEMLJ_705", "locus": "GCA000027325_00690" }, { "type": "cds", "sequence": "contig_1", "start": 387916, "stop": 390051, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTATGAAAGTAAATCTGAACCAATTCATGACTATAAATTTGGGAGTGATTTATCTCAATCAAACAGCAATAATTCGTTAGAGTCTGAACCTGTTAAGTTTAATAGTGAAACTGCACCTGATGCACACTTTGAAAGTCAATCTGAACCAGTTGATCAAGTTCAATACGACATTTATCAAAATGAAGAGCTAAAACCAACTCTTGACCAGCCTTCTTCAGATGATTATTTTGCAAAACAACCAACTGATGAAAACTATGGTTTTGATAACGACCTACCACCAGAAGTAAAACAACCTGAATCAGTTGTTGACCAGCCTTCTTCAGATGATCATTTTGCAAAACAACCTGAATCAACAACTGATAGCTACAGCTTTGACAGTGATTTACCTCAACCAACCCTTGACCAACCTTCTTTAGATGATCATGTTCAGTACAACTTTGATCACCATGAAGAGCTCAAACCAGTTGCTGAAGAACAAAATAATTATCAAGTTGGATTTGATCAAGTTCAAGCTAATCTTGATAATAATGAGGAAATACAACCAACTGCTGAAAAAGAAGTAACTACTGATTTTGAAAGTAAACAAGCCCAAGTAGTTGATAGTTACCAATTACCAATTGATACAGATCAACAAGACCAAACAACTTTTTCTTCTTCATTTGAAACTCAACCAACAGTTGAGCAGTTTGATCAAGTAAATAGCGAAGTTAACGATCAATTCAAACCTGAAATAACTAAGGAGCCAGTTTTAGAAAGTAGTTTCAATAAACAAGATGTAGTTGAAACTAGTAACTACACTAATAATTTACAGAAATTTGATATTCAGTCTGATAATAAGATAACTATCACTACCAAAAAAAGTTCACCCCAAATCCCTACAACTTTACCTATTAGTTTTGTATCCAACCGTATTGAATATAAACCAGTTGAAACTTTAGCTTTAGATAATAAAGAAAGTCAGCAAGAACAAATTACCATTAATTCCATAACTGAAGATTCCAAAACACTTGCAAAAACATTATCTGTCCAATTGCAACAAATTAATAGCTTAAATAATCAATCAATTGTTACTAGTGAATCAGTAAGACTCGATAAAAAAGATGATCAACTAACTATCAATACTGTTAATAGCGAAGACCAACAACCTAAAATTGAAGTTTTTGTCAAAGCAAAAGAACCTGTTGAAGAACACTCAATTACTCAAAATAAGCAATCGGTTGAAGATAAAAGTGAACTTGATAATTTCAATAAAAAATCAGATCTTTACAAGATTATTAGTGAATTGAAACGTGGTGAATTAAACCCTACAATTAACTTTGATGCAATCTTTCAAATGAATGATTATCAGATGTCTGTTAAACAATCATTTATCCATCTCAATGATTTTGTTACCAACTATAAGAACCAAATTAGTGAACGTTACTTAATAATTAAAAAGGAATTACAAAGTGAACTTTCCCGTTTAATTGATCAAAATGAGAACCTTAACGTTCAATTTAATAACGCTAAGAATCTAACAACACTTCAAAAAGAAGAAATGATCAGATCATTAGCTAGTGATTTTGCTATTGCTTACAAACCAAGTAATAGTTATGAACAACTGCAAAAATCTGGAGAAATTATGCGTCATGTTCAACGTGCTATCACTGAAAATGAAAAGAAGATAGAAAGCATTCAAGGTAGTTTAAAACAACTTAAAACTGTTTATAACTCTTGTTGTGAAACTATTATGAACAACATTAATAAGCTTGATAATACGCTCCGCTTTGCAAAGAAAGAAAAAGATCCTTTACTTTTAAGCAATTTTGATAGTGTAACAGATAATGGGTTAGTAGAACCTAATCAGTTAATGGATGATTTAATAGATTTTTCCAACACTTTTGATAACATCTCCAATGAACAGTTAGATGATTTTATCTATGAAAACATGGATCGTAACATCGATTTTGAATTTGAAGGTTTTAACAATGATTTTGTTGACATTGATGCCAAAGTAATGGATTCAATGAGTGCTTTTAGTGTTAATGATTTGGATATAGAAACACTAGTTCCTGATCGTACTTCCAATTTCAGTTCATTATTAGATGAAGACTTATTTGAAAGCAGTGGTGATTTTAGCCTTGATTATTAA", "aa": "MHYESKSEPIHDYKFGSDLSQSNSNNSLESEPVKFNSETAPDAHFESQSEPVDQVQYDIYQNEELKPTLDQPSSDDYFAKQPTDENYGFDNDLPPEVKQPESVVDQPSSDDHFAKQPESTTDSYSFDSDLPQPTLDQPSLDDHVQYNFDHHEELKPVAEEQNNYQVGFDQVQANLDNNEEIQPTAEKEVTTDFESKQAQVVDSYQLPIDTDQQDQTTFSSSFETQPTVEQFDQVNSEVNDQFKPEITKEPVLESSFNKQDVVETSNYTNNLQKFDIQSDNKITITTKKSSPQIPTTLPISFVSNRIEYKPVETLALDNKESQQEQITINSITEDSKTLAKTLSVQLQQINSLNNQSIVTSESVRLDKKDDQLTINTVNSEDQQPKIEVFVKAKEPVEEHSITQNKQSVEDKSELDNFNKKSDLYKIISELKRGELNPTINFDAIFQMNDYQMSVKQSFIHLNDFVTNYKNQISERYLIIKKELQSELSRLIDQNENLNVQFNNAKNLTTLQKEEMIRSLASDFAIAYKPSNSYEQLQKSGEIMRHVQRAITENEKKIESIQGSLKQLKTVYNSCCETIMNNINKLDNTLRFAKKEKDPLLLSNFDSVTDNGLVEPNQLMDDLIDFSNTFDNISNEQLDDFIYENMDRNIDFEFEGFNNDFVDIDAKVMDSMSAFSVNDLDIETLVPDRTSNFSSLLDEDLFESSGDFSLDY", "aa_hexdigest": "08e9aa9dec8c5759134d2ec131c03d21", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 81644.07640000037, "isoelectric_point": 4.2757360458374025 }, "id": "KKNAAMEMLJ_706", "locus": "GCA000027325_00691" }, { "type": "cds", "sequence": "contig_1", "start": 390564, "stop": 390959, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGAGGTAAATCACTGTCAAAGCTGTAGCTATCAGTTGTTGATTCAGGTTGTTTTGCAAAATGATCATCTGAAGAAGGTTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTTTCATCAGTTGGTTGTTTTGCAAAATAATCATCTGAAGAAGGCTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCGTTATCAAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCACTGTCAAAACCATAGTTTTCATCAGTTGGTTGAGATTCTTTAG", "aa": "MVEVNHCQSCSYQLLIQVVLQNDHLKKVGQQLIQVVLLLVVGRYQNHSFHQLVVLQNNHLKKAGQQLIQVVLLLVVGRYQNHSYLQLIQVVLLLVVNRYQNHSYLQLIQVVLLLVVNHCQNHSFHQLVEIL", "aa_hexdigest": "628564ab6aa71480e9a05bc876c5b9b7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15224.8124, "isoelectric_point": 9.153624153137208 }, "id": "KKNAAMEMLJ_707", "locus": "GCA000027325_00692" }, { "type": "cds", "sequence": "contig_1", "start": 390994, "stop": 391335, "strand": "-", "frame": 3, "gene": null, "product": "EAGR box-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418BE", "UniRef:UniRef90_UPI0001B418BE" ], "nt": "ATGGCTAAAAACAAGCAATCGGTTTTTGAAGAAAAAAACTATACCCAAACCGAACCTGAAAACATTTTTGGTGATCTTTATGATGGTAAATCCACTGTTGAAGAAGACCCTAATATTAAGGTAGCATATGATGCTGATGGTAATGGCTATTATATAGCTTTTAACAAAGAAACAGGGGTTTACTATGATCCTTATGGAGATACTGAATATGATATCTCCCAACTCTTTGATGAAAATGGGAATCCGTTTGTTTTTGATGAAAAGCAAGAAGAGAATGATTATTTAAAGTATGTTGGTAACCCTGACTATGGTTCTTATGATGAAAATGGTGAGTGGGTTTGA", "aa": "MAKNKQSVFEEKNYTQTEPENIFGDLYDGKSTVEEDPNIKVAYDADGNGYYIAFNKETGVYYDPYGDTEYDISQLFDENGNPFVFDEKQEENDYLKYVGNPDYGSYDENGEWV", "aa_hexdigest": "a6cebfdf4836e9cf17baf04b409741cd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 390895, "stop": 391335, "observations": { "mutation": [ 390996 ], "stop": [ 390996 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B418BE", "query_cov": 1.0, "subject_cov": 0.6420454545454546, "identity": 1.0, "score": 236.0, "evalue": 5.35e-78, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MAKNKQSVFEEKNYTQTEPENIFGDLYDGKSTVEEDPNIKVAYDADGNGYYIAFNKETGVYYDPYGDTEYDISQLFDENGNPFVFDEKQEENDYLKYVGNPDYGSYDENGEWVWSGYFENDQWISTKESQPTDENYGFDSDLPPEVKQPESVEDNYGFDNDLPPEVKQPESVVDNY" }, "paralog": false, "identity": 0.9931506849315068, "score": 312.0, "evalue": 1.12e-110, "description": "Internal stop codon at 390996. Nonsense mutation around 390996.", "psc": { "uniref90_id": "UniRef90_UPI0001B418BE", "product": "EAGR box-containing protein", "uniref50_id": "UniRef50_UPI0001B418BE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B418BE", "UniRef:UniRef50_UPI0001B418BE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B418BE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418BE" ], "product": "EAGR box-containing protein" } }, "id": "KKNAAMEMLJ_708", "locus": "GCA000027325_00693" }, { "type": "cds", "sequence": "contig_1", "start": 391395, "stop": 392108, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG313", "db_xrefs": [ "GO:0022857", "SO:0001217", "UniRef:UniRef50_Q50364", "UniRef:UniRef90_Q49414" ], "nt": "TTGTTCGGATGGATCTTTGGTCCTATTTGGGGTTTTATTTTTGGATTTTTATCTGATACTATTGATTGGTTAACAAAGGGTTATGTGTGGTTTTGGATGTTTGCATTGCAAAAGCCACTCTTTGCTTTTCTTGCTGGCATAGTTAAAGGGATTTATCTAGTTAGAAAAAATGCCAGTAATTACAAGGTTGATTTTTGGGTGTTACAAACACTATTAGTAACTTTTTTTGTTGTTAGTGTAACACTGCTTTTACTTTATCTAACTAATAATGAGTTTCAGCAAATTGGAACAAAAAATTTTCAAACTGCTGATCTTAGTGTTAATGTTGTTGTTTTACAAATCATAACTTTAGTTAGCTTTGTTATTTTCTTTTTAGTAACAGAAGGATTTTTAGGGTTTGTTTATGTAAAAAAGAGAAATAAAGAACAAGCACTTTTAACTATTTATAGCTTGGTTCTAATGGTTTTAATGTCAGTAGTTGTTTCTATCCTTTTGGGGACAATTGCTGCCATTGAGTTTATTACTTTTATTAATAATGGCAGACCAAGTAATAACTTTGTTTTGTATGGGGTTTATTTCTTTCTTTTACCAAGAGTATTGGTGCAAGCGTTATTATTACCTATCTATGTAGCTTTGTTTTACCCTTTAATAGGGATTGTGGAAAATAATTTAAAAAATTATTTATTACTATTTACGCTTTCATGGAAGAGTTAG", "aa": "MFGWIFGPIWGFIFGFLSDTIDWLTKGYVWFWMFALQKPLFAFLAGIVKGIYLVRKNASNYKVDFWVLQTLLVTFFVVSVTLLLLYLTNNEFQQIGTKNFQTADLSVNVVVLQIITLVSFVIFFLVTEGFLGFVYVKKRNKEQALLTIYSLVLMVLMSVVVSILLGTIAAIEFITFINNGRPSNNFVLYGVYFFLLPRVLVQALLLPIYVALFYPLIGIVENNLKNYLLLFTLSWKS", "aa_hexdigest": "42a2eb2c5f01879fe6485caf3da6f1cb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49414", "query_cov": 1.0, "subject_cov": 0.8257839721254355, "identity": 0.97, "score": 423.0, "evalue": 2.55e-148, "valid": true, "product": "Uncharacterized protein MG313", "uniref50_id": "UniRef50_Q50364", "go_ids": [ "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_Q50364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50364" ], "product": "Uncharacterized protein MG313 homolog" }, "genes": [], "id": "KKNAAMEMLJ_709", "locus": "GCA000027325_00694" }, { "type": "cds", "sequence": "contig_1", "start": 392134, "stop": 392247, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATACATTAGTAACACTAATTGAAAAGATAGAAAAGATAAAGGTTAGTGCTAATAAAACACTAGCTCAAACTAACAGTTGTAAACTCTTTAAAAGTCTCAACGGATAG", "aa": "MLNTLVTLIEKIEKIKVSANKTLAQTNSCKLFKSLNG", "aa_hexdigest": "71157081c8d4015556eb9638c6fb3a7d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4092.8644000000004, "isoelectric_point": 9.748022651672361 }, "id": "KKNAAMEMLJ_710", "locus": "GCA000027325_00695" }, { "type": "cds", "sequence": "contig_1", "start": 392303, "stop": 392491, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGTTTTTAGCATTGTTCCTTTTAGCATCATTAGTGTAATTGCTTTTTCATTAAGTCCTAAATTAAACTTAGAACAAACTAATGAGATATTTAATAAGTACTTTCAAGAGGAATTGGCAAAACCAATTACTAGTGATGAAAGTAAAACTACTTTAGATATCCCCCCACCTACTATTTTTGGTTAA", "aa": "MFVFSIVPFSIISVIAFSLSPKLNLEQTNEIFNKYFQEELAKPITSDESKTTLDIPPPTIFG", "aa_hexdigest": "d7a15b400e85a2dcbb20be8117125756", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6995.002, "isoelectric_point": 4.4943956375122065 }, "id": "KKNAAMEMLJ_711", "locus": "GCA000027325_00696" }, { "type": "cds", "sequence": "contig_1", "start": 392582, "stop": 393016, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCCATCTTTTTCATTTCGATAGCATACGTTGTGATGGTCAATGTCTTTTTTACCCACTTTGTTGATAAGAACAGTCCACTTTTCAATGTTAATAATGACCAAAATACTGTTCAGCAAAGTAATGCAGATACAACAAATCTAAACCGCTTTTTTGCTTTAAGTTCATCTAACTTGATTACCATGATCTTCCTGGCTTTTGGTGGTGCTTCTTTTTTCTTTGCTTTTCAGGGTAAACCCTATAGCTTTGCAACTAGAGGTCAAACCAATAGAGCAATGCGGTATTTACGTAGTGAATTTGGGGTCAAAGAGTTTCCCAAAATTAATGATAACTACCGTTATAAGGTAAGAATAAAATGGATCTTTTGGACTATCTTTATCTTTGTCTTTTTAAATTGTATCCCTGGTAATATAGTAAGAAGTGGCTTTTGA", "aa": "MIAIFFISIAYVVMVNVFFTHFVDKNSPLFNVNNDQNTVQQSNADTTNLNRFFALSSSNLITMIFLAFGGASFFFAFQGKPYSFATRGQTNRAMRYLRSEFGVKEFPKINDNYRYKVRIKWIFWTIFIFVFLNCIPGNIVRSGF", "aa_hexdigest": "7ff1363e8eec5e165fb015a5add2ab4e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16759.191099999993, "isoelectric_point": 10.076618003845212 }, "id": "KKNAAMEMLJ_712", "locus": "GCA000027325_00697" }, { "type": "cds", "sequence": "contig_1", "start": 393041, "stop": 393634, "strand": "-", "frame": 2, "gene": null, "product": "MPN449 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363", "UniRef:UniRef90_Q49415" ], "nt": "ATGACGTTTAGTGAAATTCTAACTAAGGTACAAAATAATCTTGACATTGTTTTTAATAACGAAACTTTAAGAACAAGGATAAAAACTGATAGTGATTTTGCCAAAACAATTTTGGCACAACTCAAATTACTTTATTTTTTAGAGGAAAAACAAAAAAGAGTTAAAACAAAAAAACCAGACCACTTTCTTTTTGGTAGTTTTCATGATAAGTTTATCCAATTAGGGCAAAACCAATTAAGTGAAAAAGAACTTAAAGCTGCTAAGTTTGATCTAACTGATGCGTTAGATTTAGCTAATTATCTTAATGTTGCTGTTAAAAACTTGTTTAATAAAGAGTTGAATAGCTTTACCAAACTTGCTGAAACTCAAGTTAAACCCGTTAGTGAGTTACAAGAAAACAATAAAACAGTTAAAGATAACCCAAGTTTTCAAACTATAAACAATTCTCAACAGCTAAACTCAGGACTTGAGAATAATATTTTGCAACAAACCCTCCAAGTTCGTGCCAGAGATCGTGCCTTTGGTAGGTTTACCTCTGAAAAACTAGTTGGTAAGATCTTTGAGTTTCAGTTTAAGAGTCAGTGGATTAAATGA", "aa": "MTFSEILTKVQNNLDIVFNNETLRTRIKTDSDFAKTILAQLKLLYFLEEKQKRVKTKKPDHFLFGSFHDKFIQLGQNQLSEKELKAAKFDLTDALDLANYLNVAVKNLFNKELNSFTKLAETQVKPVSELQENNKTVKDNPSFQTINNSQQLNSGLENNILQQTLQVRARDRAFGRFTSEKLVGKIFEFQFKSQWIK", "aa_hexdigest": "7975f1c4f06773e25fcd0ddce6176951", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 392738, "stop": 393634, "observations": { "mutation": [ 393043 ], "stop": [ 393043 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49415", "query_cov": 1.0, "subject_cov": 0.44469525959367945, "identity": 1.0, "score": 375.0, "evalue": 7.48e-128, "gene_start": 1, "gene_end": 197, "reference_start": 1, "reference_end": 197, "reference_sequence": "MTFSEILTKVQNNLDIVFNNETLRTRIKTDSDFAKTILAQLKLLYFLEEKQKRVKTKKPDHFLFGSFHDKFIQLGQNQLSEKELKAAKFDLTDALDLANYLNVAVKNLFNKELNSFTKLAETQVKPVSELQENNKTVKDNPSFQTINNSQQLNSGLENNILQQTLQVRARDRAFGRFTSEKLVGKIFEFQFKSQWIKWAQLAIFISMIAIFFISIAYVVMVNVFFTHFVDKNSPLFNVNNDQNTVQQSNADTTNLNRFFALSSSNLITMIFLAFGGASFFFAFQGKPYSFATRGQTNRAMRYLRSEFGVKEFPKINDNYRYKVRIKWIFWTIFIFVFLNCIPGNIVRSGFWNAALIIRLFSENQLISVSPLFASYLIGIAWLFVFSIVPFSIISVIAFSLSPKLNLEQTNEIFNKYFQEELAKPITSDESKTTLDIPPPTIFG" }, "paralog": false, "identity": 0.9966442953020134, "score": 579.0, "evalue": 1.55e-210, "description": "Internal stop codon at 393043. Nonsense mutation around 393043.", "psc": { "uniref90_id": "UniRef90_Q49415", "product": "MPN449 family protein", "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49415", "UniRef:UniRef50_Q50363" ] }, "pscc": { "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363" ], "product": "Uncharacterized protein MG314 homolog" } }, "id": "KKNAAMEMLJ_713", "locus": "GCA000027325_00698" }, { "type": "cds", "sequence": "contig_1", "start": 393658, "stop": 394122, "strand": "-", "frame": 3, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360", "SO:0001217", "UniRef:UniRef50_Q49416", "UniRef:UniRef90_Q49416" ], "nt": "ATGGGGGTAATTTACCAAGAAATTAACAAACTTAGCTTGTTAGGTAAAAATGAAATCAAAGATAACAAATTAGTTGAAACAGTTATTTGTGATTATCAACCTGTTCAAATTTACAAATTAACCAAAGCTCTAACAAATTCACAAATTGTAAAGGCATTTAAATATATTGATGAATTGGCAAGTACAAAACCAAATTTTGCCACCCAATTTTTAGAATTTTTTAGTGGGGAGTTGTTATTAGCATTGATGGTTAAAAGTTGTAATCCAAAGCAACTAACTAACATTAATCTAAATGTTAATCAGTTTCGCTTAATTGCTATTCAAAGTCAGTACCATAACTTTACTAGCAAAGTTTTAGTAAATATAATTAATGCAATTCAAAAATTGGATATTAAATTAAAGCACAATGATGGCTTTGCCATTCCGTTGTTAAAAAACTTTGCTTTAAGCTTTTTTACTAACTAA", "aa": "MGVIYQEINKLSLLGKNEIKDNKLVETVICDYQPVQIYKLTKALTNSQIVKAFKYIDELASTKPNFATQFLEFFSGELLLALMVKSCNPKQLTNINLNVNQFRLIAIQSQYHNFTSKVLVNIINAIQKLDIKLKHNDGFAIPLLKNFALSFFTN", "aa_hexdigest": "3684d242b37a7e23703ab1ee9e3b5517", "start_type": "ATG", "rbs_motif": null, "genes": [ "holA" ], "truncated": "3-prime", "pseudogene": { "start": 393658, "stop": 394422, "observations": { "mutation": [ 394149, 394224 ], "stop": [ 394149, 394224 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49416", "query_cov": 1.0, "subject_cov": 0.5185185185185185, "identity": 1.0, "score": 305.0, "evalue": 9.4e-103, "gene_start": 1, "gene_end": 154, "reference_start": 144, "reference_end": 297, "reference_sequence": "MTIIYGQDIGLIHQKLSQIKSNSPYKTIWFKDLKQLYDLFSQPLFGSNNEKFIVNNCSFLEKTSLTRQENLCLEKLKTTDVVLTVYTDNPFSGIKTIKSITTVFCDKLDWKSMHKAIGEVCRELNLKLDLEIIDWLANALPLNMGVIYQEINKLSLLGKNEIKDNKLVETVICDYQPVQIYKLTKALTNSQIVKAFKYIDELASTKPNFATQFLEFFSGELLLALMVKSCNPKQLTNINLNVNQFRLIAIQSQYHNFTSKVLVNIINAIQKLDIKLKHNDGFAIPLLKNFALSFFTN" }, "paralog": false, "identity": 0.9921259842519685, "score": 493.0, "evalue": 1.79e-179, "description": "Internal stop codon at 394149, 394224. Nonsense mutation around 394149, 394224.", "psc": { "uniref90_id": "UniRef90_Q49416", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q49416", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49416", "EC:2.7.7.7", "UniRef:UniRef50_Q49416", "COG:COG1466", "COG:L", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_Q49416", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49416" ], "product": "Probable DNA polymerase III subunit delta" } }, "id": "KKNAAMEMLJ_714", "locus": "GCA000027325_00699" }, { "type": "cds", "sequence": "contig_1", "start": 394222, "stop": 394551, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATAATTTATGGTCAAGATATTGGGCTAATTCACCAAAAATTAAGTCAAATCAAAAGCAATTCTCCATATAAAACTATTTGGTTTAAAGATCTTAAGCAACTTTATGATCTCTTTTCACAACCATTATTTGGTTCTAATAATGAAAAGTTTATTGTCAATAATTGCAGTTTTTTGGAAAAAACAAGCTTAACTAGACAAGAAAACTTGTGTTTAGAAAAACTAAAAACAACTGATGTAGTTTTAACTGTTTATACTGACAATCCTTTTAGTGGGATTAAAACCATTAAATCAATCACAACTGTTTTTTGTGATAAATTGGATTGA", "aa": "MTIIYGQDIGLIHQKLSQIKSNSPYKTIWFKDLKQLYDLFSQPLFGSNNEKFIVNNCSFLEKTSLTRQENLCLEKLKTTDVVLTVYTDNPFSGIKTIKSITTVFCDKLD", "aa_hexdigest": "dd63d7d34df4e7b189e0a0c82e03bf02", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12562.38229999998, "isoelectric_point": 8.422810554504398 }, "id": "KKNAAMEMLJ_715", "locus": "GCA000027325_00700" }, { "type": "cds", "sequence": "contig_1", "start": 394739, "stop": 395110, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTACCTGTTTTTAGTAGGATTTGCCTTTTCAGCACTAAGAGTTTTTATCTCAACACTGTTAAAACAGGTTTTTAAAAAACAGTTACCAGAAGATAACTTAAGTTTAACTGCACTTTTAATCATTCTTATTAGTAACCATGCTTTAAATAACTTTGGTTTTAATTTTTCTTTTCTAGCTTGTTTTGTATTGTTATTTGTCAACAAACTAAAGTTATTAAAAGCTTTAAAACCGCTTGTCAGTTCTAGTTTAATCTTGATTGTTATCAGCCCCTTAAGCTTGTATTTAAATTCAAGACTAAATGCTTTTAGTGTCTTGTTTAACCTTTTGTTTAGCCCAATTGCCTTATTTTATTTTTGTGTTAGTTGA", "aa": "MIYLFLVGFAFSALRVFISTLLKQVFKKQLPEDNLSLTALLIILISNHALNNFGFNFSFLACFVLLFVNKLKLLKALKPLVSSSLILIVISPLSLYLNSRLNAFSVLFNLLFSPIALFYFCVS", "aa_hexdigest": "9a7bc2cd2c7f55cedc503890d5f89855", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13871.699499999977, "isoelectric_point": 9.864645957946777 }, "id": "KKNAAMEMLJ_716", "locus": "GCA000027325_00701" }, { "type": "cds", "sequence": "contig_1", "start": 395772, "stop": 396242, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAACTTCAGGTTTACAAATTAAAGTTGTACCAAGATCAGCAGCTTTACTTCAATCAAAATTAGACACTGGTTTTCAACCTAGACAAGTTGAACGCACAACTGATTCAGATATCACTGTTAGTGTCTCTTCCCACGCTTCTCTTCTAGAGAAAATTAATGCTTTAAACCACCAAAGGATAATGAGTGATATTGCTCTAAAGAGTGATAACACTATAAAAAGTAGCAATTTCAGCCGTTTTTATCCTGAAAATGAGTATGTTGCAACTAAATACAGTGATCCACTTTACAGCGATACCAATCAAAGTTTAACTAGTGATCGTTTTTCTCTTGATTTTGACTACACACCAAAATCTAGAGTCAACAATTACACACCATTAAGATCAACTAATTTCCAAAATAATGCTATTTCTAACTATCGTTTTAGCAGAACACCAAGTAGTTATTATCCCTTAACAAGAAGACCATGA", "aa": "MPTSGLQIKVVPRSAALLQSKLDTGFQPRQVERTTDSDITVSVSSHASLLEKINALNHQRIMSDIALKSDNTIKSSNFSRFYPENEYVATKYSDPLYSDTNQSLTSDRFSLDFDYTPKSRVNNYTPLRSTNFQNNAISNYRFSRTPSSYYPLTRRP", "aa_hexdigest": "51c7cc00049032a9ae60be18c858b9dc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17803.540099999987, "isoelectric_point": 9.51767711639404 }, "id": "KKNAAMEMLJ_717", "locus": "GCA000027325_00702" }, { "type": "cds", "sequence": "contig_1", "start": 396282, "stop": 397424, "strand": "-", "frame": 1, "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q57081", "UniRef:UniRef90_Q57081" ], "nt": "ATGAACGATAAACAGAAAGCTAAAATAAACAAAGCCTATAACAAGCTTTTAAAAAAGATTAACAAAAGATATCCTGATGTTAGTGTTGTTTATGCCCGTGATCATAAAAATAAGGTTCATGCTCTATACCAAGATCCTGAATCAGGTAATATCTTCTCTTTAGAAAAAAGAAAGCAGTTAGCTAGTAACTATCCTTTGTTTGAATTAACTTCAGATAACCCTATTAGCTTTACAAATAACATTGTTAGCTTAAATGCTTATGATGATAAGAACAACTTGGTAACTGTTCAATATGATCAGGATAACAACACTTTCTATGACCAAAATGGTAATGTTTTGGATGTTTCTAGCTATACTGATGAGAAAAAGGTCCCTTTAATTAACTATTTAAGCAGTACTCAAACTTCTCAAGAACAACCAACCCAACAAGATTATCCTAGTATTGATGCAGGTCTTCCTAAGATAGAAGTTGATGACCAACCAAAAGCAGCTCAACACACTACTCTAGAAACTGAAAGTGAACCTGATGTCTTTGAATTAAATGACAGTTTAAATCAACCCCAACAACCTACAGAAAACTTGGGTGATGACCAGTTTGTTGAAAAAGAAGTGCCACCTACTCAACAGCTCCATCAGGATTTAGTCCACCAACAACCCGTTCAAGTTGATAGTGGATCTCAAAACCATAGTTTTAATAATTCCCCTTCTTTAAAACCACCACTAGTTAATAAACCAGCAAAGTTAGTTCAACCTGAAGTAAAACATATTCCTCAAGTTGAAGTTCAACCGAAACCTCAAATAGTTGAACCCAAAATTGAACCAAAACCAGAAGTGAAACATGTATCACATGTTGAAATTCAACCAAAACCAGAAGTGAAACCTGTTGTTGATTCAGTACCAGAAGTTAAACAACCTGAAGTAAAACATGTACCACATGTTGAAGTTCAACCAAAACCTGTTGTTGATTTAAAACCTCAAAGGATTGAACCAAGAATTGAATCAAAACCAGAAGTAATAAAACATATTCCTCAAGTTGAAGTTCAACCGAAAGCTCAAATGGTTGAACCAAGAATTGAACCAAAACCTGAAACAAAATACATCCCTCAAGTAGAATCAACTCCTCAAGTTGAAGTTCACCACTGA", "aa": "MNDKQKAKINKAYNKLLKKINKRYPDVSVVYARDHKNKVHALYQDPESGNIFSLEKRKQLASNYPLFELTSDNPISFTNNIVSLNAYDDKNNLVTVQYDQDNNTFYDQNGNVLDVSSYTDEKKVPLINYLSSTQTSQEQPTQQDYPSIDAGLPKIEVDDQPKAAQHTTLETESEPDVFELNDSLNQPQQPTENLGDDQFVEKEVPPTQQLHQDLVHQQPVQVDSGSQNHSFNNSPSLKPPLVNKPAKLVQPEVKHIPQVEVQPKPQIVEPKIEPKPEVKHVSHVEIQPKPEVKPVVDSVPEVKQPEVKHVPHVEVQPKPVVDLKPQRIEPRIESKPEVIKHIPQVEVQPKAQMVEPRIEPKPETKYIPQVESTPQVEVHH", "aa_hexdigest": "a954525c6cdec5e2a07970869b5132a3", "start_type": "ATG", "rbs_motif": null, "genes": [ "hmw3" ], "truncated": "5-prime", "pseudogene": { "start": 395979, "stop": 397424, "observations": { "mutation": [ 396284 ], "stop": [ 396284 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q57081", "query_cov": 1.0, "subject_cov": 0.6343906510851419, "identity": 0.9890000000000001, "score": 610.0, "evalue": 5.39e-215, "gene_start": 1, "gene_end": 380, "reference_start": 1, "reference_end": 380, "reference_sequence": "MNDKQKAKINKAYNKLLKKINKRYPDVSVVYARDHKNKVHALYQDPESGNIFSLEKRKQLASNYPLFELTSDNPISFTNNIVSLNAYDDKNNLVTVQYDQDNNTFYDQNGNVLDVSSYTDEKKVPLINYLSSTQTSQEQPTQQDYPSIDAGLPKIEVDDQPKAAQHTTLETESEPDVFELNDSLNQPQQPTENLGDDQFVEKEVPPTQQLHQDLVHQQPVQVDSGSQNHSFNNSPSLKPPLVNKPAKLVQPEVKHIPQVEVQPKPQIVEPKIEPKPEVKHVSHVEIQPKPEVKPVVDSVPEVKQPEVKHVPHVEVQPKPVVDLKPQRIEPRIESKPEVIKHIPQVEVQPKAQMVEPRIEPKPETKYIPQVESTPQVEVHHWKPEVKTEYQPQQPLPTSGLQIKVVPRSAALLQSKLDTGFQPRQVERTTDSDITVSVSSHASLLEKINALNHQRIMSDIALKSDNTIKSSNFSRFYPENEYVATKYSDPLYSDTNQSLTSDRFSLDFDYTPKSRVNNYTPLRSTNFQNNAISNYRFSRTPSSYYPLTRRPWRLTNISSYRSSFHSPTRLSSFRRTSLPFSSSYDGLRRYPSRSYWSKDF" }, "paralog": false, "identity": 0.9958419958419958, "score": 834.0, "evalue": 1.15e-305, "description": "Internal stop codon at 396284. Nonsense mutation around 396284.", "psc": { "uniref90_id": "UniRef90_Q57081", "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "uniref50_id": "UniRef50_Q57081", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q57081", "UniRef:UniRef50_Q57081", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q57081", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q57081" ], "product": "Cytadherence high molecular weight protein 3" } }, "id": "KKNAAMEMLJ_718", "locus": "GCA000027325_00703" }, { "type": "cds", "sequence": "contig_1", "start": 397439, "stop": 397783, "strand": "-", "frame": 2, "gene": null, "product": "P32 adhesin", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q49417", "UniRef:UniRef90_Q49417" ], "nt": "ATGCAACAACGCCCTGGTTTTAACCAACCAAACCAACAGTTCCAACCACACAATAATTTCAACCCAAGGATGAACCCAAACATGCAGCGCCCTGGGTTTAATCCAAACATGCAACAACGCCCTGGTTTTAACCAACCAAACCAACAGTTCCAACCACACAATAATTTCAACCCAAGGATGAACCCAAACATGCAACGCCCTGGATTTAACCAACCCCATCCTAACCAATTTGCACAACCAAATAACTTTAATCCAAACATGCAACAACGCCCTGGGTTTAATCCAAATATGCAACAACGTCCTAACCCATCACAATTGATGCCAAAAGGCGGTTTAAAACCCTAA", "aa": "MQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNPNMQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNQPHPNQFAQPNNFNPNMQQRPGFNPNMQQRPNPSQLMPKGGLKP", "aa_hexdigest": "8ed87d76763a2a42be80d8ee7e295471", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 397439, "stop": 398083, "observations": { "mutation": [ 398080 ], "stop": [ 398080 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49417", "query_cov": 1.0, "subject_cov": 0.40714285714285714, "identity": 0.9390000000000001, "score": 110.0, "evalue": 1.56e-27, "gene_start": 1, "gene_end": 114, "reference_start": 167, "reference_end": 280, "reference_sequence": "MELNGFLRYKKLFIVLALLFTTILIVSLSLLAFALVVKTNGSELGVVFHQTEDNTTVIQGRSIVEQPWFIPTVAGSFGFSALAIILGLAIGLPIVKRKEKRLLEEKERQEQIAEQLQRISDQQEQQTVEIDPQQSQAQPSQPQVQQPLQPQFQQRVPLLRPAFNPNMQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNPNMQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNQPHPNQFAQPNNFNPNMQQRPGFNPNMQQRPNPSQLMPKGGLKP" }, "paralog": false, "identity": 0.9906542056074766, "score": 233.0, "evalue": 6.03e-78, "description": "Internal stop codon at 398080. Nonsense mutation around 398080.", "psc": { "uniref90_id": "UniRef90_Q49417", "product": "P32 adhesin", "uniref50_id": "UniRef50_Q49417", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49417", "UniRef:UniRef50_Q49417", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q49417", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49417" ], "product": "P32 adhesin" } }, "id": "KKNAAMEMLJ_719", "locus": "GCA000027325_00704" }, { "type": "cds", "sequence": "contig_1", "start": 397795, "stop": 398004, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGGTCTTAAAAGCGGAACACGCTGTTGAAACTGTGGTTGAAGGGGTTGTTGAACTTGGGGTTGGGAAGGCTGAGCTTGGGATTGTTGAGGATCAATTTCAACTGTTTGTTGTTCTTGTTGATCTGAGATTCTTTGTAATTGCTCAGCAATCTGTTCTTGGCGTTCCTTCTCTTCAAGTAAACGTTTTTCTTTGCGCTTTACAATAG", "aa": "MQVLKAEHAVETVVEGVVELGVGKAELGIVEDQFQLFVVLVDLRFFVIAQQSVLGVPSLQVNVFLCALQ", "aa_hexdigest": "2c2914b64d56c59a9343b0f1daf73d56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7510.7654999999995, "isoelectric_point": 4.375886344909667 }, "id": "KKNAAMEMLJ_720", "locus": "GCA000027325_00705" }, { "type": "cds", "sequence": "contig_1", "start": 398078, "stop": 398281, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGTTAAATGGATTTTTGAGATACAAAAAACTTTTTATAGTTCTTGCTTTACTTTTTACAACAATCCTAATTGTTAGTTTATCGTTACTAGCATTTGCACTTGTTGTGAAAACTAATGGTAGTGAACTAGGGGTTGTTTTTCATCAAACAGAAGATAATACAACTGTAATTCAGGGCAGATCAATTGTTGAACAGCCCTGA", "aa": "MELNGFLRYKKLFIVLALLFTTILIVSLSLLAFALVVKTNGSELGVVFHQTEDNTTVIQGRSIVEQP", "aa_hexdigest": "9d7993b6226778def9d21e86b92e2024", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7450.733800000002, "isoelectric_point": 6.532982826232909 }, "id": "KKNAAMEMLJ_721", "locus": "GCA000027325_00706" }, { "type": "cds", "sequence": "contig_1", "start": 398298, "stop": 398834, "strand": "-", "frame": 1, "gene": null, "product": "MG319/MPN454 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010869428.1", "SO:0001217", "UniParc:UPI0000139608", "UniRef:UniRef100_P47561", "UniRef:UniRef50_P47561", "UniRef:UniRef90_P47561" ], "nt": "ATGCGCTTGTTTCGATTTCTCTTTAAGCTTTGCTTTCTTTTATTGGTATTGGTAGGCTTTGCTTATCTTTTCTTAGCTATCTTTTACTTTGGTTCTCTAAACCCATTTGAGTTAGCACAACCAATGGATGTTTTCAACAGGTTCTTTTCAAAAGAAGCGTTGGATAACATCTCTTCAAACAATGGTGCAACTGCAACTGCTCAAACAAGTAGCTTATTACAACTTTTAGAAGGTTCAAGTAATGGTTTGGATAACCGTTTTCCAACTGAAAAAAGTGCATTTTATGCTATCCCAGGATATGTAGATTTTTTGAAAAATGCTAAGTTGCCTGGATTTGTTGAACAGTTTACACCTTATCTAACTAAGTATGTAATTCCCCTAGGAATGGCATTTGTTAGTGGTTTAATAGGTACTTTAATTGTTAACTTCTTTCTCAATAAAATCACTCGTTCAATTAAAAGAAGAAAAAGGAACATGAAAAAGCAAGAACAAGAAGAGTATTATGATGATTCAAGATCAAGAAGAAAAAGGAACTAA", "aa": "MRLFRFLFKLCFLLLVLVGFAYLFLAIFYFGSLNPFELAQPMDVFNRFFSKEALDNISSNNGATATAQTSSLLQLLEGSSNGLDNRFPTEKSAFYAIPGYVDFLKNAKLPGFVEQFTPYLTKYVIPLGMAFVSGLIGTLIVNFFLNKITRSIKRRKRNMKKQEQEEYYDDSRSRRKRN", "aa_hexdigest": "b27dbdd9f01959166de9f05ca322577a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139608", "ncbi_nrp_id": "WP_010869428.1", "uniref100_id": "UniRef100_P47561", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139608", "RefSeq:WP_010869428.1", "UniRef:UniRef100_P47561" ] }, "ips": { "uniref100_id": "UniRef100_P47561", "uniref90_id": "UniRef90_P47561" }, "psc": { "uniref90_id": "UniRef90_P47561", "product": "MG319/MPN454 family protein", "uniref50_id": "UniRef50_P47561", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47561", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47561" ], "product": "Uncharacterized protein MG319" }, "genes": [], "id": "KKNAAMEMLJ_722", "locus": "GCA000027325_00707" }, { "type": "cds", "sequence": "contig_1", "start": 399043, "stop": 399294, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTCACATCTTATTATGGGCTTTTGTAATTAGTATTGTAGCGAGCTTATTGAGCTTTATTTTGAACTTTACAGTGTTTGCTTCTACAACAAACCTACTTGATAGAATTATCCCTGGCTTATCTTTAATAGTAGGGGGAATCTTTTCATTAATTTCAGTTTATTTTGTGAGTTTACAGATCCGTAATGAACAAGATTTGATTAAATACTATGAAAGTGAAGATTATGAAATGGCAAGAAGACAGAGCTGA", "aa": "MFHILLWAFVISIVASLLSFILNFTVFASTTNLLDRIIPGLSLIVGGIFSLISVYFVSLQIRNEQDLIKYYESEDYEMARRQS", "aa_hexdigest": "661d40e5410c0b23eadfa555bff4be3f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9552.070799999996, "isoelectric_point": 5.045847511291503 }, "id": "KKNAAMEMLJ_723", "locus": "GCA000027325_00708" }, { "type": "cds", "sequence": "contig_1", "start": 399346, "stop": 399798, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG320", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47562", "UniRef:UniRef90_P47562" ], "nt": "ATGATTAATTCAACAAAAGGCTATATTGATCAAAACGGTTTAGCAGCAAAGCAGTTTGTACAAACAAAACAGCTTTCTGTAATCCGTTTAACTTTTATGGTAGCTGCTTTTGGGATCTTTTTTATCTTTCTTGTTGCATTAACAGTACAACAATTGCTTTCAAGAAGCACACTAATTGATTTAGCTAGTGATTTTAGAACACTTAGTACTATTGCAGTTATTACTAGTTTTGTCTCTTTAATTCTTTATTTTGTTACTGCTTTTAAATTAAGGAATCCCAACACTAGTTTGACATGGTTTTGGGCCTTAATCATTACTGATGTGATTAGTTATGGGATAACCCTTGGGATCTTATTAACTTTAGCAACGACATTTTCAAAACAAGTTAACTTTGAAGCTAATGACATTGTTTATGCTTTTTTAGGTGCTAGTTTGGTGTTTGGTTCAGTGTGA", "aa": "MINSTKGYIDQNGLAAKQFVQTKQLSVIRLTFMVAAFGIFFIFLVALTVQQLLSRSTLIDLASDFRTLSTIAVITSFVSLILYFVTAFKLRNPNTSLTWFWALIITDVISYGITLGILLTLATTFSKQVNFEANDIVYAFLGASLVFGSV", "aa_hexdigest": "ed0cab07a6b88a66d50d846d23b06080", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 399043, "stop": 399798, "observations": { "mutation": [ 399348 ], "stop": [ 399348 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47562", "query_cov": 1.0, "subject_cov": 0.5244755244755245, "identity": 1.0, "score": 273.0, "evalue": 1.14e-90, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MINSTKGYIDQNGLAAKQFVQTKQLSVIRLTFMVAAFGIFFIFLVALTVQQLLSRSTLIDLASDFRTLSTIAVITSFVSLILYFVTAFKLRNPNTSLTWFWALIITDVISYGITLGILLTLATTFSKQVNFEANDIVYAFLGASLVFGSVWGLSALPSQKRRYQQTQTLFHILLWAFVISIVASLLSFILNFTVFASTTNLLDRIIPGLSLIVGGIFSLISVYFVSLQIRNEQDLIKYYESEDYEMARRQSWRSALFFGAWLISSFMNLVYFILRIILITKNFSRV" }, "paralog": false, "identity": 0.9960159362549801, "score": 473.0, "evalue": 8.47e-172, "description": "Internal stop codon at 399348. Nonsense mutation around 399348.", "psc": { "uniref90_id": "UniRef90_P47562", "product": "Uncharacterized protein MG320", "uniref50_id": "UniRef50_P47562", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47562", "UniRef:UniRef50_P47562", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47562" ], "product": "Uncharacterized protein MG320" } }, "id": "KKNAAMEMLJ_724", "locus": "GCA000027325_00709" }, { "type": "tRNA", "sequence": "contig_1", "start": 399868, "stop": 399958, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(gga)", "amino_acid": "Ser", "anti_codon": "gga", "score": 70.9, "nt": "GGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAAAACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 399868, 399870 ], "id": "KKNAAMEMLJ_13", "locus": "GCA000027325_00710" }, { "type": "tRNA", "sequence": "contig_1", "start": 399960, "stop": 400048, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 78.7, "nt": "GGAGATTTACCCAAGTGGCTGAAGGGGGCGCTCTCGAAAAGCGTTAGGTGGTTATCCACGCGTGGGTTCAAATCCCACAATCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 399994, 399996 ], "id": "KKNAAMEMLJ_14", "locus": "GCA000027325_00711" }, { "type": "cds", "sequence": "contig_1", "start": 400305, "stop": 400709, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGCTGCATCCCAAAACAATGTCTTTAATCAAATTGAAAATAAACTTCAAAATAACACAACAACTAACGGAAAAGACTGGTGTAGTTGCATAACAAGTGCTGATCTTTTTAAAGATGATCCCTATGTCATTAAGAACTTTGGTACTAATGGTAATAACGGTACATCCGCTTCATTAGCATTCACTAAAAAAGCATTAAGCTTACTGAAATTTTTAGTTGATAATAAGGTATTGAAAGCTGATAAAGTTAAAGAAGCAATTAAAGATCCTGATAAATATCTATCAAAAAGAAGCAAGATAGATGACAACAAACCAGCAAATGTATCAGATATCCACATTGGTTATGAATTGAAAGATCTTTATGACAACGCTGCTCAACTTAACCGATTTAACAGCATTTGA", "aa": "MLAASQNNVFNQIENKLQNNTTTNGKDWCSCITSADLFKDDPYVIKNFGTNGNNGTSASLAFTKKALSLLKFLVDNKVLKADKVKEAIKDPDKYLSKRSKIDDNKPANVSDIHIGYELKDLYDNAAQLNRFNSI", "aa_hexdigest": "0c05512b4442278a62ee9499d205f5fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14949.676499999981, "isoelectric_point": 8.798983573913574 }, "id": "KKNAAMEMLJ_725", "locus": "GCA000027325_00712" }, { "type": "cds", "sequence": "contig_1", "start": 401901, "stop": 402923, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAAAAAAGATACTTGTTGCTCGGTTCTACACTGACAGTTAGTGCAGCACTAATCCTAAGTGCTTGTGCATCCTCACAGTTAAACAAAGGGGTGTTTTTCACCTCTTCTTCAGCAGATTTGTTAAAGAACAACTCTGTTCCATTGAGCATGTTTAACGTTTCACCCACTTCATCTTTTTTTGGTAATGCTTATGCAGGGTTAACTAACTATGTTGCTACTGGTACAAACCGTGATGATGGGGTTAATGTTACTGACCAAATTAAAGAAAAATTAGTGTTAGAACTTGCTTTATCAGTTACTGGCTATAAAAAGACAACACACAGTGGCCTTAAAGGTAGAGCACAAAAAAACGGTTCAACAGATTCTTCTGATGGATCTAGTAAGAATGATTACACTAAGTTCAATGAGTGGGATGCAATAGGCACAATTTATCGTGATAGTTCAAAGTCTATTACTGAAGATCCTAACTATCACAAGATAACCCAAGAAGCAACCAGATATGAATTTACCATTAACACTAGTTTGAGCTGGGTTGATAATGCTGGTAGAGAAGTCAAACAAAACAACCAACCAGTTAAACTCTCCTCTAAAGACTTTGAAAGGGGTTTTGAGACCTATATTCTCTCTTCTAACTTAGGTTTTAACCGGAATGGTTATTTCATTGACCTAATGGGTCTTGATGTTGAAAAAACCGTTGGTATGGATAAAACCAACGGTTCTAGTGATGGCAATGGTAAAGGTATTGAGATCACTGATGAAAATTATGATGTTGAAAACTATAGAAGTGTAGATGATAACAAGTTTAATGTTTATCTAACTTCTCCTTTTCCTTTCTTTCTATCAATGATGTCAAAGGAATTCTTCTTTCCAATTCCTCACACCCATCCTAAGGTAAAAGCCCTTAAACTAGGTAAAGATTCACCGCTTAAATATAACCAAAATAACAACAGAAAGATTTTAGATCAAGCAAATACTAACTTTGATGGTATCTATGGAGGGGGTGTTAATGCTTGA", "aa": "MKLKKRYLLLGSTLTVSAALILSACASSQLNKGVFFTSSSADLLKNNSVPLSMFNVSPTSSFFGNAYAGLTNYVATGTNRDDGVNVTDQIKEKLVLELALSVTGYKKTTHSGLKGRAQKNGSTDSSDGSSKNDYTKFNEWDAIGTIYRDSSKSITEDPNYHKITQEATRYEFTINTSLSWVDNAGREVKQNNQPVKLSSKDFERGFETYILSSNLGFNRNGYFIDLMGLDVEKTVGMDKTNGSSDGNGKGIEITDENYDVENYRSVDDNKFNVYLTSPFPFFLSMMSKEFFFPIPHTHPKVKALKLGKDSPLKYNQNNNRKILDQANTNFDGIYGGGVNA", "aa_hexdigest": "f598b9970a192e0d7d34215a830f041a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37644.521600000015, "isoelectric_point": 8.31663112640381 }, "id": "KKNAAMEMLJ_726", "locus": "GCA000027325_00713" }, { "type": "tRNA", "sequence": "contig_1", "start": 403134, "stop": 403218, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 64.0, "nt": "GCACTCGTGGCGGAATGGTAGACGCGCTAGACTTAGGATCTAGTTTCATCTAGAAGTGGGGGTTCAAGTCCCTTCGGGTGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 403198, 403200 ], "id": "KKNAAMEMLJ_25", "locus": "GCA000027325_00714" }, { "type": "tRNA", "sequence": "contig_1", "start": 403224, "stop": 403299, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 81.4, "nt": "GCATCTTTAGCTCAGTTGGTAGAGCAAATGACTCTTAATCATTGGGTCGTGGGTTCGATCCCCTCAAGATGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 403293, 403295 ], "id": "KKNAAMEMLJ_24", "locus": "GCA000027325_00715" }, { "type": "tRNA", "sequence": "contig_1", "start": 403306, "stop": 403381, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 90.9, "nt": "GCCGACTTAGCTCAGTTGGTAGAGCAATTGACTTGTAATCAATAGGTCGTAGGTTCAAATCCTATAGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 403346, 403348 ], "id": "KKNAAMEMLJ_23", "locus": "GCA000027325_00716" }, { "type": "tRNA", "sequence": "contig_1", "start": 403383, "stop": 403458, "strand": "-", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 81.7, "nt": "GGATTGTTAGCTCAGCTGGGAGAGCATATGCCTTACAAGCATAGGGTCGGGGGTTCGATCCCCTCACAATCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 403423, 403425 ], "id": "KKNAAMEMLJ_22", "locus": "GCA000027325_00717" }, { "type": "tRNA", "sequence": "contig_1", "start": 403467, "stop": 403541, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 80.2, "nt": "GCCGAATTAGCTCAAGGGCAGAGCGATGCACTCGTAATGCATAGGCTACAGGTTCAATTCCTGTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 403507, 403509 ], "id": "KKNAAMEMLJ_21", "locus": "GCA000027325_00718" }, { "type": "tRNA", "sequence": "contig_1", "start": 403544, "stop": 403620, "strand": "-", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 58.9, "nt": "GGCGCGTTGGTGAAGAGACTTAACACACACGCCTTTCACGCGTGCATTCACGGGTTTGAATCCCGTACGCGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 403584, 403586 ], "id": "KKNAAMEMLJ_20", "locus": "GCA000027325_00719" }, { "type": "tRNA", "sequence": "contig_1", "start": 403627, "stop": 403701, "strand": "-", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 84.0, "nt": "GGCCACATAGCTCAGCGGTAGAGCAACCGGCTGTTAACCGGTTGGTCACAGGTTCGATCCCTGTTGTGGCCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 403667, 403669 ], "id": "KKNAAMEMLJ_19", "locus": "GCA000027325_00720" }, { "type": "cds", "sequence": "contig_1", "start": 403723, "stop": 405033, "strand": "-", "frame": 3, "gene": "trkG", "product": "putative cation transporter MG322", "db_xrefs": [ "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001", "SO:0001217", "UniRef:UniRef50_P75323", "UniRef:UniRef90_P47564" ], "nt": "TTGTTTAACTTTCACAAGAAGGAACAATACAGTTTTTATGAAAAGTTAATGTTGCAATCAGAACGAGGTGGTTCTAAGCTAGGTAATACTAGTGAGATGATCTTAGTATCTATCATCTTTCTTTTTATCGTTGAACTAATTTATGGATTTTTATATGGTATTTTGTTTTATTTCATCCCAGGCTTTGAACCTGCTAACTTGTTTGCAGATCATGCAAAAGTTTCAACTCAATTAAAAGCTTTAGTAGTTGATTCAAACCAAACAATAGCAGCTTTTAATGATATTAATAAGGCTTTTCAAGCAGGTTTTTTCCATTCCTTATCAGCAGTTAATAATGCTGGGATAGATCTGATAGGGGGTAGTTCTTTTGTTCCTTATAGAAATGGACTTGGTATTATTATTCAGTGGTTAACTATTAGCCAAATTATCTTTGGGGGAATTGGTTATCCTTGTTTGTTTGATGGCTTTGAAGCCATTAAAAAAAAGATTAAGTATGGTAGACACACAAAACACCAATTTAGTCTATTTACCAAGTTGACAGTAATTACTAATATCGTTGTAATCCTGCTTTTTTTCACCTTACTTTTAATGGTGGAATTTATTGCTAGTGATAGTTTAACTAACACTATTGTTAATTTTAGTGATGAAAAAAAGAGTTTAATAAATACCCAATTGCAATCACAATCTAACCAAGCAATCCATGCGTCAGTTTTTGGTAATAACCCTAATGCAAGTAGGGTAATGCAGCTCTTTTTTATGGTTATTTCATCCCGTTCAGCAGGTTTTAGTGTTTTCCCTGTTGCTAGTGAGATTCAAACTACAAAAATAATTATTGCATTGGCAATGTTTATTGGTGCTAGTCCCTCTTCTACTGCTGGGGGGATTAGAACAACTACGCTAGCAGTAATCTTTTTAGCTCTAGTTGCTAAGTTTAAAGGTCAAAAGGAAGTAAAAGCATTTAAGCGTTCAATCGATCAAACTACAGTAATAGATGCTTTTTTAGTACTAATCATAAGCTTAATTGCAGTTTTACTAACAGCTGTTCTTCTACCTTTAAGTATGGAACAACCAGTTAGTTTCATTGATGCTTTATTTGAAACAACTAGTGCTTTTGGAACAGTTGGACTTTCAAGTGGAGCTACTGTTAACATTGCTTTAGATCCAAATAGAAATACCTTTAATTTCCTTGCTTTATGTCTATTAATGGTTATGGGACAGGTTGGTGTGTCCAGTTCTGTGCTAACTTTTGTTAGAAAACATCCCAAAGCAAATAGTTATTCATATCCTAAGGAAGCTGTTAAAATTGGCTAG", "aa": "MFNFHKKEQYSFYEKLMLQSERGGSKLGNTSEMILVSIIFLFIVELIYGFLYGILFYFIPGFEPANLFADHAKVSTQLKALVVDSNQTIAAFNDINKAFQAGFFHSLSAVNNAGIDLIGGSSFVPYRNGLGIIIQWLTISQIIFGGIGYPCLFDGFEAIKKKIKYGRHTKHQFSLFTKLTVITNIVVILLFFTLLLMVEFIASDSLTNTIVNFSDEKKSLINTQLQSQSNQAIHASVFGNNPNASRVMQLFFMVISSRSAGFSVFPVASEIQTTKIIIALAMFIGASPSSTAGGIRTTTLAVIFLALVAKFKGQKEVKAFKRSIDQTTVIDAFLVLIISLIAVLLTAVLLPLSMEQPVSFIDALFETTSAFGTVGLSSGATVNIALDPNRNTFNFLALCLLMVMGQVGVSSSVLTFVRKHPKANSYSYPKEAVKIG", "aa_hexdigest": "63f9e400e6396f9a432f9b16a268dc94", "start_type": "TTG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 403723, "stop": 405333, "observations": { "mutation": [ 405039, 405234 ], "stop": [ 405039, 405234 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47564", "query_cov": 1.0, "subject_cov": 0.7716814159292036, "identity": 0.998, "score": 808.0, "evalue": 1.01e-292, "gene_start": 1, "gene_end": 436, "reference_start": 130, "reference_end": 565, "reference_sequence": "MTRRQNQMVKLTTWLKKIGWGETITQRIFCFYIYCILFGSLLLFLPIALQDNYQKVVSYGIDWQGKRFEQKTDYNFLDALFLSTSAFSDTGLSTVVVSKTYSIFGQIVLAVLLQLGGIGFVVIAFLAWRLFNFHKKEQYSFYEKLMLQSERGGSKLGNTSEMILVSIIFLFIVELIYGFLYGILFYFIPGFEPANLFADHAKVSTQLKALVVDSNQTIAAFNDINKAFQAGFFHSLSAVNNAGIDLIGGSSFVPYRNGLGIIIQWLTISQIIFGGIGYPCLFDGFEAIKKKIKYGRHTKHQFSLFTKLTVITNIVVILLFFTLLLMVEFIASDSLTNTIVNFSDEKKSLINTQLQSQSNQAIHASVFGNNPNASRVMQLFFMVISSRSAGFSVFPVASEIQTTKIIIALAMFIGASPSSTAGGIRTTTLAVIFLALVAKFKGQKEVKAFKRSIDQTTVIDAFLVLIISLIAVLLTAVLLPLSMEQPVSFIDALFETTSAFGTVGLSSGATVNIALDPNRNTFNFLALCLLMVMGQVGVSSSVLTFVRKHPKANSYSYPKEAVKIG" }, "paralog": false, "identity": 0.996268656716418, "score": 1023.0, "evalue": 0.0, "description": "Internal stop codon at 405039, 405234. Nonsense mutation around 405039, 405234.", "psc": { "uniref90_id": "UniRef90_P47564", "gene": "TrkG", "product": "Uncharacterized cation transporter MG322", "uniref50_id": "UniRef50_P75323", "cog_id": "COG0168", "cog_category": "P", "go_ids": [ "GO:0005886", "GO:0008324", "GO:0030001" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47564", "UniRef:UniRef50_P75323", "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001" ] }, "pscc": { "uniref50_id": "UniRef50_P75323", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75323" ], "product": "Uncharacterized cation transporter MG322 homolog" } }, "id": "KKNAAMEMLJ_727", "locus": "GCA000027325_00721" }, { "type": "cds", "sequence": "contig_1", "start": 405456, "stop": 406139, "strand": "+", "frame": 3, "gene": "trkA", "product": "putative protein MG323", "db_xrefs": [ "COG:COG0569", "COG:PT", "GO:0006813", "GO:0008324", "RefSeq:WP_010869432.1", "SO:0001217", "UniParc:UPI0000139619", "UniRef:UniRef100_P47565", "UniRef:UniRef50_P75322", "UniRef:UniRef90_P47565" ], "nt": "ATGAAACGTGCAGATTTTTGCATTATTGGTTTAGGTAGATTTGGGATGCAGGTTGCACAATCACTAAAAGAAAACAACTTTAACTTACTTTTAATTGATCTTGATGATAAAAAAACTGACACCGCCTCGCAACAGTTTGATTATGTTATTTGTTGTGATGCTAGTAATCTAACTGCTTTAGAAGAGTTACAAATTGATGAATTTGCTGGGGTTATTGTTGGGGTTACCAACATAGAAGCGAGCATCATGATATGTGCTAATTTAAGGGAATTAGGACAAAAAAACATTATTGCTAAAGCCAAAAATGAAGTACATAAAAGGGTGTTAAGCACAATGGGAATTAGAGAAGCTTTGATCCCTGAAAAGATTGTTGGTAAAAATCTGGTTATCCGCTTAATCCATGGGCTTGAAAATGAAATTATCAACCTTGGTAATGAGATAATTTTCATCCGTTCAGCAGTTAACAACAAGGCTTTTTTTAACAAAAGGTTAGAAGAGATTAACTTTAGGCAAAACACCGATGCTAACATTATCTCCATCATGCGCAGTAATAAAACTGTTGTTTTTCCTTTAGGACCAAATACTGAGATCCAACCAGGGGATATTATCACTGCAGTTTGTCAACAAAAAAGTTTAAATAAGTACTTAAATTACATCAATCCTAAAACCAAAAATAAAAATTAA", "aa": "MKRADFCIIGLGRFGMQVAQSLKENNFNLLLIDLDDKKTDTASQQFDYVICCDASNLTALEELQIDEFAGVIVGVTNIEASIMICANLRELGQKNIIAKAKNEVHKRVLSTMGIREALIPEKIVGKNLVIRLIHGLENEIINLGNEIIFIRSAVNNKAFFNKRLEEINFRQNTDANIISIMRSNKTVVFPLGPNTEIQPGDIITAVCQQKSLNKYLNYINPKTKNKN", "aa_hexdigest": "bc98d52b4d6eeab8face0013d7c32f12", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139619", "ncbi_nrp_id": "WP_010869432.1", "uniref100_id": "UniRef100_P47565", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139619", "RefSeq:WP_010869432.1", "UniRef:UniRef100_P47565" ] }, "ips": { "uniref100_id": "UniRef100_P47565", "uniref90_id": "UniRef90_P47565" }, "psc": { "uniref90_id": "UniRef90_P47565", "gene": "TrkA", "product": "Uncharacterized protein MG323", "uniref50_id": "UniRef50_P75322", "cog_id": "COG0569", "cog_category": "PT", "go_ids": [ "GO:0008324", "GO:0006813" ] }, "pscc": { "uniref50_id": "UniRef50_P75322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75322" ], "product": "Uncharacterized protein MG323 homolog" }, "genes": [ "trkA" ], "id": "KKNAAMEMLJ_728", "locus": "GCA000027325_00722" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 406179, "stop": 406519, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 251.3, "evalue": 2.2e-27, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "GCTGTCGGTTAGTTGCTGCTTTAAAGATATATTTAAAGTAGAGGAAAGTCCATGCTTGCACTTGCTGGAATGCAAGTAGTGTTTGTGTAAATCAAAGCGATAAGATTTAGGATGTGATAGCCATAACGACATAACTAGTCTAAGGTTAATACTATGATGATGTTTTGAAAGTGCCACAGAGACGAGCTTGGTGAAAACCAGGTATGAAACGCGGTAAACTCCACAAGCAAGAAACCTAAATTTTGGTAAGGGAATGTTGCTTTAAGAACAAATAATTAAAGTAATGACAGCAATTGCTGTAGATAAATAACTGACTTTAAAACAGAACATGGCTTATTGAC", "id": "KKNAAMEMLJ_41", "locus": "GCA000027325_00723" }, { "type": "tmRNA", "sequence": "contig_1", "start": 406542, "stop": 406929, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 406625, "stop": 406705, "aa": "DKENNEVLVDPNLIINQQASVNFAFA", "nt": "GACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAA" }, "nt": "GGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA", "id": "KKNAAMEMLJ_37", "locus": "GCA000027325_00724" }, { "type": "cds", "sequence": "contig_1", "start": 407080, "stop": 407238, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATTTTTGGTGTTATTAGACCAACAGTTTTTTGAAAGAGATACACAAAAAATTCAATCAGCAACGCGGTTTAGCTTGTTTTTTAATGTTGAAAATAACAATGGTGTTTATCTCTCTAAATTAAATAGTTTTGGTGTATTTGCTACCGCTTGA", "aa": "MVLFLVLLDQQFFERDTQKIQSATRFSLFFNVENNNGVYLSKLNSFGVFATA", "aa_hexdigest": "e984ef0aaf7f2c60a8f119f77d0000b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6009.7965, "isoelectric_point": 5.971413612365722 }, "id": "KKNAAMEMLJ_729", "locus": "GCA000027325_00725" }, { "type": "cds", "sequence": "contig_1", "start": 407470, "stop": 407739, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTCTAAAAAGAAAAATAGTGGTGGTGGTTGGATTAATAAGATATCTGATAAACTCAGTGAAATAAATGTCGCTGAGTATCAAGTTGTTTCCAAACACAGTAACTATCTGTTTTACAGCAGGTTTGGCTTACTTGATACTTTTGTTTACTTTTTATTTTTTCTCTGCTTTTTTCTAAACAACGTTTTGTTTTTAGCTGGCGTTTTTCACACTAAACAATTCACTTTTAACGAAGTTAACGGCTTTAATCAGTTTTACCTTTTTTGA", "aa": "MLSKKKNSGGGWINKISDKLSEINVAEYQVVSKHSNYLFYSRFGLLDTFVYFLFFLCFFLNNVLFLAGVFHTKQFTFNEVNGFNQFYLF", "aa_hexdigest": "fd133266959697cd97b6eddd4a3a3c52", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10493.975099999987, "isoelectric_point": 9.131962776184086 }, "id": "KKNAAMEMLJ_730", "locus": "GCA000027325_00726" }, { "type": "cds", "sequence": "contig_1", "start": 407759, "stop": 408406, "strand": "-", "frame": 2, "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "db_xrefs": [ "COG:COG0006", "COG:E", "EC:3.4.11.9", "GO:0004177", "GO:0005829", "GO:0006508", "GO:0008237", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47566", "UniRef:UniRef90_P47566" ], "nt": "TTGCCTAGTGAGATTCAAGCCATTGAAAAAGCAGTTGATATTACAAGAAAAGTAGCAGTTAAGTTAAAGCGTTTTATTAAACCAAAAATGACTGAGCTTTTCATTTCACAATGGATTACTAATGAGCTTGTGAAGCAGGGTGGGGCTAAAAACTCATTTGATCCTATAGTAGCTACTGGTAAAAACGGTGCTAACCCACATCACAAACCAACCAAAACAATAGTTAAGGAAGGTGATTTTATCACTTGTGATTTTGGCACCATTTACAACGGTTATTGCTCTGATATTACAAGAACCTTTTTAGTTGGTAAAAAACCTAAAAGTGCAAAGTTATTATCTGCATACAAAAAAGTTGAAGAGGCTAATTTGGCTGGTATAAATGCAGTAAACACTACTTTAACAGGTTCACAAGTTGATAAGGTGTGCCGCGATATTATTGAAAACTCTGAGTTTAAAGACTTTTTTGTGCATAGTACTGGTCATGGCGTTGGTATTGATATCCATGAAATGCCAAATGTTTCCCAATCTTACAACAAGTTATTGTGTGAAAATGGGGTTGTAACCATTGAACCTGGGATCTATATTCCTAACCTTGGTGGAATTAGGATAGAAGATATGGTACTTGTTAAAAAAGAAAAATCAGTTTGA", "aa": "MPSEIQAIEKAVDITRKVAVKLKRFIKPKMTELFISQWITNELVKQGGAKNSFDPIVATGKNGANPHHKPTKTIVKEGDFITCDFGTIYNGYCSDITRTFLVGKKPKSAKLLSAYKKVEEANLAGINAVNTTLTGSQVDKVCRDIIENSEFKDFFVHSTGHGVGIDIHEMPNVSQSYNKLLCENGVVTIEPGIYIPNLGGIRIEDMVLVKKEKSV", "aa_hexdigest": "c1439c6283541e84a60c4a4643c85ca5", "start_type": "TTG", "rbs_motif": null, "genes": [ "pepP" ], "truncated": "both", "pseudogene": { "start": 407729, "stop": 408706, "observations": { "mutation": [ 407761, 408469, 408694 ], "stop": [ 407761, 408469, 408694 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47566", "query_cov": 1.0, "subject_cov": 0.6073446327683616, "identity": 0.995, "score": 432.0, "evalue": 4.99e-151, "gene_start": 1, "gene_end": 215, "reference_start": 130, "reference_end": 344, "reference_sequence": "MISELQQKITVLKDLLKTNKADAILIGSDQNRFWLTNFPSSAGWLIITSNKAKLFIDGRYYEAARNFINPIVEVELFVSFKQVKAFCESNGINHLLIEGDYLTFNYQDWIQAICKQYTVINAQEIRRVKLPSEIQAIEKAVDITRKVAVKLKRFIKPKMTELFISQWITNELVKQGGAKNSFDPIVATGKNGANPHHKPTKTIVKEGDFITCDFGTIYNGYCSDITRTFLVGKKPKSAKLLSAYKKVEEANLAGINAVNTTLTGSQVDKVCRDIIENSEFKDFFVHSTGHGVGIDIHEMPNVSQSYNKLLCENGVVTIEPGIYIPNLGGIRIEDMVLVKKEKSVWLSKSIPRAF" }, "paralog": false, "identity": 0.9907692307692307, "score": 641.0, "evalue": 5.37e-236, "description": "Internal stop codon at 407761, 408469, 408694. Nonsense mutation around 407761, 408469, 408694.", "psc": { "uniref90_id": "UniRef90_P47566", "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "ec_ids": [ "3.4.11.9" ], "uniref50_id": "UniRef50_P47566", "cog_id": "COG0006", "cog_category": "E", "go_ids": [ "GO:0005829", "GO:0004177", "GO:0046872", "GO:0008237", "GO:0006508" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47566", "EC:3.4.11.9", "UniRef:UniRef50_P47566", "COG:COG0006", "COG:E", "GO:0005829", "GO:0004177", "GO:0046872", "GO:0008237", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47566", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47566" ], "product": "Putative Xaa-Pro aminopeptidase" } }, "id": "KKNAAMEMLJ_731", "locus": "GCA000027325_00727" }, { "type": "cds", "sequence": "contig_1", "start": 408467, "stop": 408634, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGATGGTAGGTATTATGAAGCAGCAAGGAACTTTATTAATCCTATTGTTGAAGTTGAATTATTTGTTAGTTTTAAACAAGTTAAAGCTTTTTGTGAATCTAATGGTATCAATCACCTTTTAATTGAGGGTGACTATCTTACCTTTAACTATCAGGATTGA", "aa": "MFIDGRYYEAARNFINPIVEVELFVSFKQVKAFCESNGINHLLIEGDYLTFNYQD", "aa_hexdigest": "b613da37f195ebf056763eb64660f9e1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6483.252399999999, "isoelectric_point": 4.63461742401123 }, "id": "KKNAAMEMLJ_732", "locus": "GCA000027325_00728" }, { "type": "cds", "sequence": "contig_1", "start": 408692, "stop": 408793, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTGAATTACAACAAAAAATTACAGTTCTTAAAGATCTTTTAAAAACCAATAAAGCTGATGCTATCCTAATTGGTTCTGATCAAAACCGCTTTTGA", "aa": "MISELQQKITVLKDLLKTNKADAILIGSDQNRF", "aa_hexdigest": "630404c05c9db6326146da9695555f66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.3478999999998, "isoelectric_point": 8.189692878723147 }, "id": "KKNAAMEMLJ_733", "locus": "GCA000027325_00729" }, { "type": "cds", "sequence": "contig_1", "start": 408793, "stop": 408954, "strand": "-", "frame": 3, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "COG:COG0267", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_009885961.1", "SO:0001217", "UniParc:UPI0000132FB9", "UniRef:UniRef100_P47567", "UniRef:UniRef50_P47567", "UniRef:UniRef90_P47567" ], "nt": "ATGGCTGTTAAAAGAAGCACACGACTAGGTTGTAATGAATGTTCTGAGATTAATTATTTAACCTTTAAAAACGTCAAGAAAAATCCAGAAAAACTAGCACTTAATAAGTTTTGTTCACGTTGTCGTAAGGTAGTATTGCACAAAGAGGTAAAACGTAAATAA", "aa": "MAVKRSTRLGCNECSEINYLTFKNVKKNPEKLALNKFCSRCRKVVLHKEVKRK", "aa_hexdigest": "57bb09256735e72ca12fdf19d5cbdcfd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000132FB9", "ncbi_nrp_id": "WP_009885961.1", "uniref100_id": "UniRef100_P47567", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000132FB9", "RefSeq:WP_009885961.1", "UniRef:UniRef100_P47567" ] }, "ips": { "uniref100_id": "UniRef100_P47567", "uniref90_id": "UniRef90_P47567" }, "psc": { "uniref90_id": "UniRef90_P47567", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P47567", "cog_id": "COG0267", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47567", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47567" ], "product": "Large ribosomal subunit protein bL33A" }, "genes": [ "rpmG" ], "id": "KKNAAMEMLJ_734", "locus": "GCA000027325_00730" }, { "type": "cds", "sequence": "contig_1", "start": 408971, "stop": 409465, "strand": "-", "frame": 2, "gene": "degV", "product": "DegV domain-containing protein MG326", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "SO:0001217", "UniRef:UniRef50_P75312", "UniRef:UniRef90_P47568" ], "nt": "TTGGTCGAAGATATAAAGGCATTGGTTGACAAGGGTTGTGATAATCAAACAATTAAAGCAAAGGTTGAATCACATAAACAAAATATCCTTTCTGCAGTTACTTTAAAAAACCTAGTGCAAATGCGTAAAGGTGGGAGAATCTCTGGTTTGAAAAAGTTCATTACTACTTTACTAAGAGTTAAACCTATTATCTTGTTTGACAAGGGAGTTAATACTCTAGGAGCAAAAGTTTTTAGTTTTAGTCAAGCGGTTGAGAAGATATTTGGTTTTGTTAAAACTAAGTTTGGTGATAACTACAAAATTAAACGGATCGGTTTTTGCTATTCTTTTTGTAAAAATTACGCTAATGAAATTAAAAAAATAATTACTGATTTCATTGAGCATAATAAGATTAACTTTCAAAATGAAATTGAAAATGCTTTCATTACAAGTGTGATTATTGTTCACACTGGCATTGATGCTTTTTCTATTAGTTTACTTATTGACAATAAATAA", "aa": "MVEDIKALVDKGCDNQTIKAKVESHKQNILSAVTLKNLVQMRKGGRISGLKKFITTLLRVKPIILFDKGVNTLGAKVFSFSQAVEKIFGFVKTKFGDNYKIKRIGFCYSFCKNYANEIKKIITDFIEHNKINFQNEIENAFITSVIIVHTGIDAFSISLLIDNK", "aa_hexdigest": "a4cdaab366d37b9349a46bdab2e1a3b7", "start_type": "TTG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "3-prime", "pseudogene": { "start": 408971, "stop": 409765, "observations": { "mutation": [ 409468 ], "stop": [ 409468 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47568", "query_cov": 1.0, "subject_cov": 0.5559322033898305, "identity": 0.9940000000000001, "score": 318.0, "evalue": 4.44e-108, "gene_start": 1, "gene_end": 164, "reference_start": 132, "reference_end": 295, "reference_sequence": "MKKTAIITDSTASIKPGEINGVYILPLQVIVDGEKSFRDGIEIDYDHVHKLLKENPHGLNISTSLPRQSDLLKIFEEIKTKYDRFIFLPLSKGLSGTYDMLVQLAKELSEQNKDKEFLVFETSDIAISLKWLVEDIKALVDKGCDNQTIKAKVESHKQNILSAVTLKNLVQMRKGGRISGLKKFITTLLRVKPIILFDKGVNTLGAKVFSFSQAVEKIFGFVKTKFGDNYKIKRIGFCYSFCKNYANEIKKIITDFIEHNKINFQNEIENAFITSVIIVHTGIDAFSISLLIDNK" }, "paralog": false, "identity": 0.9962121212121212, "score": 516.0, "evalue": 2.56e-188, "description": "Internal stop codon at 409468. Nonsense mutation around 409468.", "psc": { "uniref90_id": "UniRef90_P47568", "gene": "DegV", "product": "DegV domain-containing protein MG326", "uniref50_id": "UniRef50_P75312", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47568", "UniRef:UniRef50_P75312", "COG:COG1307", "COG:I", "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P75312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75312" ], "product": "DegV domain-containing protein MG326 homolog" } }, "id": "KKNAAMEMLJ_735", "locus": "GCA000027325_00731" }, { "type": "cds", "sequence": "contig_1", "start": 409466, "stop": 409858, "strand": "-", "frame": 2, "gene": "degV", "product": "DegV domain-containing protein MG326", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "SO:0001217", "UniRef:UniRef50_P75312", "UniRef:UniRef90_P47568" ], "nt": "ATGAAGAAAACAGCTATCATCACTGATTCAACTGCTTCTATTAAACCTGGGGAGATTAACGGCGTTTACATCTTGCCTTTACAAGTAATTGTTGATGGTGAAAAAAGCTTTCGTGATGGGATAGAAATTGATTATGATCATGTTCATAAACTTCTAAAAGAAAACCCACATGGACTTAACATCTCAACTTCCTTACCACGTCAAAGTGATCTTCTTAAGATCTTTGAAGAGATTAAAACTAAGTATGATCGCTTTATTTTTCTGCCTTTAAGTAAAGGTTTAAGTGGTACTTATGATATGTTGGTTCAATTAGCAAAAGAGCTTAGTGAACAAAATAAAGACAAAGAATTTTTAGTGTTTGAAACTAGTGATATTGCTATTTCTCTAAAATGA", "aa": "MKKTAIITDSTASIKPGEINGVYILPLQVIVDGEKSFRDGIEIDYDHVHKLLKENPHGLNISTSLPRQSDLLKIFEEIKTKYDRFIFLPLSKGLSGTYDMLVQLAKELSEQNKDKEFLVFETSDIAISLK", "aa_hexdigest": "3bb653f8ccc2d08412055983b432fbcb", "start_type": "ATG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "5-prime", "pseudogene": { "start": 409163, "stop": 409858, "observations": { "mutation": [ 409468 ], "stop": [ 409468 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47568", "query_cov": 1.0, "subject_cov": 0.4406779661016949, "identity": 1.0, "score": 257.0, "evalue": 2.29e-84, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKKTAIITDSTASIKPGEINGVYILPLQVIVDGEKSFRDGIEIDYDHVHKLLKENPHGLNISTSLPRQSDLLKIFEEIKTKYDRFIFLPLSKGLSGTYDMLVQLAKELSEQNKDKEFLVFETSDIAISLKWLVEDIKALVDKGCDNQTIKAKVESHKQNILSAVTLKNLVQMRKGGRISGLKKFITTLLRVKPIILFDKGVNTLGAKVFSFSQAVEKIFGFVKTKFGDNYKIKRIGFCYSFCKNYANEIKKIITDFIEHNKINFQNEIENAFITSVIIVHTGIDAFSISLLIDNK" }, "paralog": false, "identity": 0.9956709956709957, "score": 446.0, "evalue": 2.43e-161, "description": "Internal stop codon at 409468. Nonsense mutation around 409468.", "psc": { "uniref90_id": "UniRef90_P47568", "gene": "DegV", "product": "DegV domain-containing protein MG326", "uniref50_id": "UniRef50_P75312", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47568", "UniRef:UniRef50_P75312", "COG:COG1307", "COG:I", "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P75312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75312" ], "product": "DegV domain-containing protein MG326 homolog" } }, "id": "KKNAAMEMLJ_736", "locus": "GCA000027325_00732" }, { "type": "cds", "sequence": "contig_1", "start": 409871, "stop": 410677, "strand": "-", "frame": 2, "gene": "menH", "product": "Putative esterase/lipase 2", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0052689", "RefSeq:WP_010869433.1", "SO:0001217", "UniParc:UPI000012A1B5", "UniRef:UniRef100_Q49418", "UniRef:UniRef50_P75311", "UniRef:UniRef90_Q49418" ], "nt": "ATGCTAACGAGTAATAAAAATACTCTTTTTAACTCAATATTTGCCTTTAAACCTAAAAAAAGAAAAAACGTTTTTATTTTTTTACATGGATTTGGAAGCGAATATGCCAGTTTTAGTCGTATTTTTAGCTTGTTTAAAAAGAAAAAGTGGCCTTTCTTTACTTTTAATTTTCCCGGTCATGGAGACAATGAATCAACAGATACAGATCAATTAAAACTAAACCACTTTGTTGATTTGGTTTGTGATTTTATTGTTCAAAAAAAACTAAATAACGTCATTTTAATAGGTCATAGTATGGGAGGTGCTGTGGCTGTTTTAGTTAACAAAGTTATACCACTAAAAATTAAGGCTTTAATATTAGTAGCTCCTATGAATCAAACTTCTTTCTCAGTTAATAAAAAGCGGATTTTAGATACTTTTTTTAAGCGTAATAACAGCAACCATAAGGATTTTGTTGAACATGAAGAAAAACGTAAATCACTTTTGAAAATAGCTATCAATGCATTTAAAAAAAGAACTACCTTTAAAACACTTTACAGTGATATGGTTCAAAACGCTAAATATGGTAATGACTCACTTGAAAGGGCTTATGAAATGATTGGTAATAAACCTACCTTAGTTATTTTAGGAGCTAATGATATTGTCACGCCAACCAAAGCTTCTGTAGACTATCTAGCTAATAAAAGTGACAAGATTATTTTTAAGGTTATTGATGGTGTTGGTCATTCACCACACGACTCAGCGCCTAAACTTTTTTTTGATTATGTGCTAGAGTTTCTAGATAATTTAAAGAAACAAAGGTACTAA", "aa": "MLTSNKNTLFNSIFAFKPKKRKNVFIFLHGFGSEYASFSRIFSLFKKKKWPFFTFNFPGHGDNESTDTDQLKLNHFVDLVCDFIVQKKLNNVILIGHSMGGAVAVLVNKVIPLKIKALILVAPMNQTSFSVNKKRILDTFFKRNNSNHKDFVEHEEKRKSLLKIAINAFKKRTTFKTLYSDMVQNAKYGNDSLERAYEMIGNKPTLVILGANDIVTPTKASVDYLANKSDKIIFKVIDGVGHSPHDSAPKLFFDYVLEFLDNLKKQRY", "aa_hexdigest": "3bffeff7e1f08834f23bc25cb54b35ef", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012A1B5", "ncbi_nrp_id": "WP_010869433.1", "uniref100_id": "UniRef100_Q49418", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012A1B5", "RefSeq:WP_010869433.1", "UniRef:UniRef100_Q49418" ] }, "ips": { "uniref100_id": "UniRef100_Q49418", "uniref90_id": "UniRef90_Q49418" }, "psc": { "uniref90_id": "UniRef90_Q49418", "gene": "MenH", "product": "Putative esterase/lipase 2", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75311", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0052689" ] }, "pscc": { "uniref50_id": "UniRef50_P75311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75311" ], "product": "Putative esterase/lipase 2" }, "genes": [ "menH" ], "id": "KKNAAMEMLJ_737", "locus": "GCA000027325_00733" }, { "type": "cds", "sequence": "contig_1", "start": 410664, "stop": 411134, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATGCTAATGAACAATACCAAGATTTACTCTCTGCTTTTGAAGAAACAAACAGTGAATTGGAGAAAGCCAAGCAAAGTTTAAGTGCATCTGATAGTGAAAATAATCAGTTAAAACAACAAATAAATTCACTTGAAAATGCTAAAAAAGAACTTCAATTTACTCCAGTTACCAGTGATGAACATCTTGATGAACTAGAAACATTAAAAATTGAAAAAGAACAACTTTTTTTAGAAAACCAAGCACTGCAAAATCAGTTGCAATATTTCAATGACATTAGTGCTAATCAAACTGAAGAGATCAAAGAAGCTAGTGATGAAGATAAACCAGTTGAAATAAAAAAACCACGAATTAAAAAACGTGATTTTGTTATCCAAAATAAGGATGATAAACTCGCTAAACTTTCTAAGAAAGAACGCATCCAAGCATATGCAGAACGTTTAGCTAAAATCAATGCTAACGAGTAA", "aa": "MNDANEQYQDLLSAFEETNSELEKAKQSLSASDSENNQLKQQINSLENAKKELQFTPVTSDEHLDELETLKIEKEQLFLENQALQNQLQYFNDISANQTEEIKEASDEDKPVEIKKPRIKKRDFVIQNKDDKLAKLSKKERIQAYAERLAKINANE", "aa_hexdigest": "97b4ac2e7bea49e75e88f8111ed3bb99", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18115.818199999998, "isoelectric_point": 4.790356254577637 }, "id": "KKNAAMEMLJ_738", "locus": "GCA000027325_00734" }, { "type": "cds", "sequence": "contig_1", "start": 411549, "stop": 412934, "strand": "-", "frame": 1, "gene": "yhgE", "product": "putative protein MG328", "db_xrefs": [ "COG:COG1511", "COG:S", "SO:0001217", "UniRef:UniRef50_Q49419", "UniRef:UniRef90_Q49419" ], "nt": "ATGGCAGTAGATAAGGAACTTGAAATTAGTGATTTTGATAATGAGCTTGATGAAAAAACACTTTTAAAAGAATTAGTACAAAGAACTAATAATATCCTTTTTTCACCATCTAAAATTACTGCTATTCCCTTTGAAAGAAATTTATTGGAGAAAACATTTTTTGGTACTGTTGATGAAGCTGAGAAAGAAAAGTCAATAGTTTCTTTTTTTAATTGGATGATAGATTTAAAGGTTTTAGATAAAAAATGGGATAAGAATGTTTTAAATCATTATGCAAATCAACTTAAAACTAGAGAGGAAGAGCAACAGACAGTTGATCAAACTATGGCTTTTCAAGAAGTTGATGATCAATCAGTTTTAACCAAGGAAATTAAAACTGGTTTTCAGGAACTAAAACCATCAGTGATTACTGCTGAAGATGATAAGGATGAGATAAAACCAGAAGCTACTAAACAAGTATCTTTTGAAGAGTTGTTTAACCAACCTAGTGAAGAGATTAATGAAACAAAAAAACCAGAAGTTCAAATATTTTCAACTGATAAGGTTAAAGAACCTGAACAATTTGATGATTTCTATTCCATTGAAAATTTAACCAAAGCTATCAATCCAGTTCATAAAACGATCCAATATGATCAGAATGATGATCAACCTTTTGTTGTTAAAAGGATCTTAAAAGAGCAACATCCAACAAAAAAAGTTGATGAATTGGATGACTATAATAACAAAGAGCTTTTATTGGAAAATGCTGATTTAAAAAAACAAATCGATGATCTTAAAGAGAATAATAATGATCAAATATTTGATTTAGAACAGGAAATCGATGATCTTAAAAGGAGGTTATCTGAGGAAAAAAGTAAGCACTTACATACCAAGAAATTACAGGATGATCTTTTGCAAGAAAATAGGGATTTATATGAGCAACTGCAAAATAAACCAGTTGCTATTAATCCTTTAAGTGATGAAGTTAATGAAGAGTTGGAGAATTTAAAACAAGAAAAAGCTTTGCTTTCAGATCAATTAGATGCATTGAAAAATAAGAGCAGTAATGTTCAACAACAATTAGCTTTATTACCAGTTTTAAACAACCAAATAAACGAACTTCAAAACCAGTTACTAACAGCAAGAGAAGCAAACCAAAGATTGGATCTAGTTGAACAAGAAAATGATTTTTTGAAAAATGAACTGAAAAAACTCCATGACAACACATCTAATGATGAGAATGAAAAATATGATGATCTTTTAAATCAGTATGAACTACTTTTTGATGAGAATGAAACAAAATTTGATAAATTGCAAGTGAAACAACAAGCGCTAAACTTAGATTATCAAAAAACAATTTCAGCACTTAAACATGAAAATGATGTTTTATTAGATGAAATTGAGTGA", "aa": "MAVDKELEISDFDNELDEKTLLKELVQRTNNILFSPSKITAIPFERNLLEKTFFGTVDEAEKEKSIVSFFNWMIDLKVLDKKWDKNVLNHYANQLKTREEEQQTVDQTMAFQEVDDQSVLTKEIKTGFQELKPSVITAEDDKDEIKPEATKQVSFEELFNQPSEEINETKKPEVQIFSTDKVKEPEQFDDFYSIENLTKAINPVHKTIQYDQNDDQPFVVKRILKEQHPTKKVDELDDYNNKELLLENADLKKQIDDLKENNNDQIFDLEQEIDDLKRRLSEEKSKHLHTKKLQDDLLQENRDLYEQLQNKPVAINPLSDEVNEELENLKQEKALLSDQLDALKNKSSNVQQQLALLPVLNNQINELQNQLLTAREANQRLDLVEQENDFLKNELKKLHDNTSNDENEKYDDLLNQYELLFDENETKFDKLQVKQQALNLDYQKTISALKHENDVLLDEIE", "aa_hexdigest": "39a7ce403a1940804be2cc5321a31ad8", "start_type": "ATG", "rbs_motif": null, "genes": [ "yhgE" ], "truncated": "5-prime", "pseudogene": { "start": 411246, "stop": 412934, "observations": { "mutation": [ 411551 ], "stop": [ 411551 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49419", "query_cov": 1.0, "subject_cov": 0.6097883597883598, "identity": 0.998, "score": 813.0, "evalue": 2.83e-291, "gene_start": 1, "gene_end": 461, "reference_start": 1, "reference_end": 461, "reference_sequence": "MAVDKELEISDFDNELDEKTLLKELVQRTNNILFSPSKITAIPFERNLLEKTFFGTVDEAEKEKSIVSFFNWMIDLKVLDKKWDKNVLNHYANQLKTREEEQQTVDQTMAFQEVDDQSVLTKEIKTGFQELKPSVITAEDDKDEIKPEATKQVSFEELFNQPSEEINETKKPEVQIFSTDKVKEPEQFDDFYSIENLTKAINPVHKTIQYDQNDDQPFVVKRILKEQHPTKKVDELDDYNNKELLLENADLKKQIDDLKENNNDQIFDLEQEIDDLKRRLSEEKSKHLHTKKLQDDLLQENRDLYEQLQNKPVAINPLSDEVNEELENLKQEKALLSDQLDALKNKSSNVQQQLALLPVLNNQINELQNQLLTAREANQRLDLVEQENDFLKNELKKLHDNTSNDENEKYDDLLNQYELLFDENETKFDKLQVKQQALNLDYQKTISALKHENDVLLDEIEWTRSKDNDFNNTKNSFEEQKKALDEKLNGLTIQNQQLQDKIAELEEWNEEKSNLNTNQLVNLQQQLKDSQNLFNVAQDKLATLEEVNLALNEKINDLEDELSGSENSNNLLAKLQADHEILQESYGKLKTDFEKLKKNKLNDANEQYQDLLSAFEETNSELEKAKQSLSASDSENNQLKQQINSLENAKKELQFTPVTSDEHLDELETLKIEKEQLFLENQALQNQLQYFNDISANQTEEIKEASDEDKPVEIKKPRIKKRDFVIQNKDDKLAKLSKKERIQAYAERLAKINANE" }, "paralog": false, "identity": 0.998220640569395, "score": 1056.0, "evalue": 0.0, "description": "Internal stop codon at 411551. Nonsense mutation around 411551.", "psc": { "uniref90_id": "UniRef90_Q49419", "gene": "YhgE", "product": "Uncharacterized protein MG328", "uniref50_id": "UniRef50_Q49419", "cog_id": "COG1511", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49419", "UniRef:UniRef50_Q49419", "COG:COG1511", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_Q49419", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49419" ], "product": "Uncharacterized protein MG328" } }, "id": "KKNAAMEMLJ_739", "locus": "GCA000027325_00735" }, { "type": "cds", "sequence": "contig_1", "start": 412973, "stop": 414319, "strand": "-", "frame": 2, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "RefSeq:WP_009885980.1", "SO:0001217", "UniParc:UPI0000129EF8", "UniRef:UniRef100_P47571", "UniRef:UniRef50_P75309", "UniRef:UniRef90_P47571" ], "nt": "GTGTTTACTGTTGCAATAATAGGTCGTACAAACGTTGGTAAATCAACTTTATTTAATCGCTTAATTCAAAAGCCAATGGCTATTGTTTCAGATACTCCTAACACGACAAGAGATAGGATCTTTGGAATTGGTGAATGGTTAAAAAGAAAGATAGCTTTTATTGACACAGGTGGATTAATTGCAAAACAAACTCCCTTGCAACAACTAATTGCATTACAAGTACAAGCAGCACTTAGTCAAGCCAAAGCTATTATTTTTTTAGTGTCACTACAAGAACAACTAAATAGCGATGATTTTTATGTAGCTAAGGTTCTTAAAAAAAATAAGGATAAACCAGTAATACTAGTGGTAAATAAAGCTGAAAATTTTAACCCTAAAACTGCTGAAGAAACATTAAAAGATTATTACAGCTTAGGGTTTGGAAGACCAGTTGTTATTAGTGCTGCTCATGGAATTGGAATTGGTGATTTAATGGATCTCTTAGTTAAACAAAATCAATTACTCCCTAATGAAAATAATGATGATTTAGCAAAAATAAGGTTTTGTGTAATTGGCAAACCAAATGTTGGTAAAAGCTCACTTATTAATCAACTTGTAAAACAAAATCGTGTTTTAGTTTCAAATGAGAGTGGTACAACTAGAGATGCTATTGATGTTCCTTTAAAAGTTAATGGTGAAAAATTTCTTTTGATTGATACTGCTGGTATTAAAAGAAAAGGCAAAATTAACATGGGAATTGAAACCGCATCTTACATCAAAACAAAGCTAGCTATTGCCCGTAGTAACGTTATCCTTTTGATGGTAGATGGATCAAAACCTATTAGTGAACAAGATGAAGTGATTGGTGGATTGGCACAAGCTGCACTTATCCCTGTTATTATTCTTGTGAATAAATGGGATCTAGTTTTAAAAAACAATAACACCACAAATGCATATAAAAAAATGCTGAAATTGCATTTTAAACACCTCGATTTTGCGCCTGTTTTGTTTATTAGTGTTTTGAAAAATCAGCGCTTAAATACTATTTTTGAACAACTTAAAATTATTCAAAGCCAACTTGAAACTAAGGTTGCAACACCACTTTTAAATGATGTTATTCAGCAAGCACAACTTTACAATCAACCTCCACTTTTTAAAGGCAAGCGTTTGCAGATAACTTACGCAGTACAAACCAAAAGTCAAATTCCCCATTTTGTTTTATTTTGCAATGACCCTAAGTATCTTCATTTTTCTTATGCACGTTTTTTAGAGAATAAGATTAGAGAAAACTTTGGTTTTAATTCTGTACCTATTAGCCTTTACTTTAAATCAAAAAACGCACGGATAAGAACAAAGCCAGAAGTTTAA", "aa": "MFTVAIIGRTNVGKSTLFNRLIQKPMAIVSDTPNTTRDRIFGIGEWLKRKIAFIDTGGLIAKQTPLQQLIALQVQAALSQAKAIIFLVSLQEQLNSDDFYVAKVLKKNKDKPVILVVNKAENFNPKTAEETLKDYYSLGFGRPVVISAAHGIGIGDLMDLLVKQNQLLPNENNDDLAKIRFCVIGKPNVGKSSLINQLVKQNRVLVSNESGTTRDAIDVPLKVNGEKFLLIDTAGIKRKGKINMGIETASYIKTKLAIARSNVILLMVDGSKPISEQDEVIGGLAQAALIPVIILVNKWDLVLKNNNTTNAYKKMLKLHFKHLDFAPVLFISVLKNQRLNTIFEQLKIIQSQLETKVATPLLNDVIQQAQLYNQPPLFKGKRLQITYAVQTKSQIPHFVLFCNDPKYLHFSYARFLENKIRENFGFNSVPISLYFKSKNARIRTKPEV", "aa_hexdigest": "0df3e9498c716aaf6a2a8376a4a18112", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129EF8", "ncbi_nrp_id": "WP_009885980.1", "uniref100_id": "UniRef100_P47571", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129EF8", "RefSeq:WP_009885980.1", "UniRef:UniRef100_P47571" ] }, "ips": { "uniref100_id": "UniRef100_P47571", "uniref90_id": "UniRef90_P47571" }, "psc": { "uniref90_id": "UniRef90_P47571", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75309", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P75309", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75309" ], "product": "GTPase Der" }, "genes": [ "der" ], "id": "KKNAAMEMLJ_740", "locus": "GCA000027325_00736" }, { "type": "cds", "sequence": "contig_1", "start": 414323, "stop": 414769, "strand": "-", "frame": 2, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "COG:COG0283", "COG:F", "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431", "SO:0001217", "UniRef:UniRef50_P47572", "UniRef:UniRef90_P47572" ], "nt": "GTGTATTATAACAATGCTGATATTACAACAGTTATTACAACCCAATCAGTTGCTAACATTGCTAGTAAAATAGCTGTTGATCCTAACATTAGAAAAATTGCAGTTATTAAACAACAGAAACTAGCAGAAAATAAAAACATAGTGATGGATGGTAGAGACATAGGAACAGTAGTTTTAAAAAATGCTCAATTGAAGTTTTTTTTAGATGCTAAAGTTGAAATTAGAGCGCAGCGAAGATTACAAGATATGGGAATTTCTCTATCAAATGAAAAAAAACTAAAGGAACTAATTCAAGAATTAAAGCAACGTGATCAAATTGATAGTTCTAGAACTGCAGACCCATTAAAAAAAGCCCAGGACGCTATTTATCTTGACACTTCTGAACTAAGTTTTGATGCAGTAGTAAAACAAACCCTCAAAGAAGCAAAGAAGGTTTTTAAACTTTAA", "aa": "MYYNNADITTVITTQSVANIASKIAVDPNIRKIAVIKQQKLAENKNIVMDGRDIGTVVLKNAQLKFFLDAKVEIRAQRRLQDMGISLSNEKKLKELIQELKQRDQIDSSRTADPLKKAQDAIYLDTSELSFDAVVKQTLKEAKKVFKL", "aa_hexdigest": "d73d108ff95f14321377c73a3df6cde7", "start_type": "GTG", "rbs_motif": null, "genes": [ "cmk" ], "truncated": "3-prime", "pseudogene": { "start": 414323, "stop": 414976, "observations": { "mutation": [ 414790, 414970 ], "stop": [ 414790, 414970 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47572", "query_cov": 1.0, "subject_cov": 0.6820276497695853, "identity": 0.993, "score": 269.0, "evalue": 7.1e-90, "gene_start": 1, "gene_end": 148, "reference_start": 70, "reference_end": 217, "reference_sequence": "MNWQIAIDGPSSSGKSSVAKKIAEELDFFYFSSGKMYRAFAYVMQVNRLNIDLFLKIINQINWRFEKDAVYYNNADITTVITTQSVANIASKIAVDPNIRKIAVIKQQKLAENKNIVMDGRDIGTVVLKNAQLKFFLDAKVEIRAQRRLQDMGISLSNEKKLKELIQELKQRDQIDSSRTADPLKKAQDAIYLDTSELSFDAVVKQTLKEAKKVFKL" }, "paralog": false, "identity": 0.9907834101382489, "score": 405.0, "evalue": 3.93e-146, "description": "Internal stop codon at 414790, 414970. Nonsense mutation around 414790, 414970.", "psc": { "uniref90_id": "UniRef90_P47572", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_P47572", "cog_id": "COG0283", "cog_category": "F", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47572", "EC:2.7.4.25", "UniRef:UniRef50_P47572", "COG:COG0283", "COG:F", "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ] }, "pscc": { "uniref50_id": "UniRef50_P47572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47572" ], "product": "Cytidylate kinase" } }, "id": "KKNAAMEMLJ_741", "locus": "GCA000027325_00737" }, { "type": "cds", "sequence": "contig_1", "start": 415034, "stop": 415243, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557EBC", "UniRef:UniRef90_UPI0000557EBC" ], "nt": "ATGCTAAACAGCATGAAACTTATTAAAGTTATCACTAAGTTCTTAAAAATAGCCTTATTAGCATTAAACAAATTAGGTTCGATGTTATCAATGCTTTTTTTAAAAGCAAAACTTTCAGTTGAAAAAACTGTTAGTATCTTGTTGTATTCTTTTAAATCACTAGTTAAATTGCTTGTTTTTTTTAATCATTGTTTCTCATTAGCTTCATAA", "aa": "MLNSMKLIKVITKFLKIALLALNKLGSMLSMLFLKAKLSVEKTVSILLYSFKSLVKLLVFFNHCFSLAS", "aa_hexdigest": "d66a2511a7f38532eb179d83cb5663cc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557EBC", "query_cov": 0.8260869565217391, "subject_cov": 1.0, "identity": 1.0, "score": 95.1, "evalue": 1.43e-24, "valid": true, "uniref50_id": "UniRef50_UPI0000557EBC" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557EBC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557EBC" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7781.673299999997, "isoelectric_point": 10.252229881286617 }, "id": "KKNAAMEMLJ_742", "locus": "GCA000027325_00738" }, { "type": "cds", "sequence": "contig_1", "start": 415291, "stop": 415488, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B4188E", "UniRef:UniRef90_UPI0001B4188E" ], "nt": "TTGATTAATTACCATCCTTTTAGTGAATTTAATAAAAATCCAGTTAAACATCACACTGAACCAAATAAACTGTTTAAAACACTTCAGGAATTAATAGTTGATTTAAAAAATACTGACTTTAAATTATTAGAAGAAAAAGTTGATAGGATGTGGTTAAACGCAGCATATAACCAAACTTCTAGCGGTTATGAAAGCTGA", "aa": "MINYHPFSEFNKNPVKHHTEPNKLFKTLQELIVDLKNTDFKLLEEKVDRMWLNAAYNQTSSGYES", "aa_hexdigest": "1918e9d197e5c33827961012463bbd57", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 415193, "stop": 415623, "observations": { "deletion": [ 415221 ], "mutation": [ 415293, 415524 ], "stop": [ 415293, 415524 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B4188E", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 0.985, "score": 135.0, "evalue": 2e-39, "gene_start": 1, "gene_end": 65, "reference_start": 46, "reference_end": 110, "reference_sequence": "MGRVEKFRFYRQSFDNNKIVKKALINAQKNTESWKKQLNKINQKILINYHPFSEFNKNPVKHHTEPNKLFKTLQELIVDLKNTDFKLLEEKVDRMWLNAAYNQTSSGYESWISDDKGIEKINHLSKFYEANEKQWLKKNKQFN" }, "paralog": false, "identity": 0.972027972027972, "score": 275.0, "evalue": 2.44e-97, "description": "Internal stop codon at 415293, 415524. Frameshift due to deletion around 415221. Nonsense mutation around 415293, 415524.", "psc": { "uniref90_id": "UniRef90_UPI0001B4188E", "uniref50_id": "UniRef50_UPI0001B4188E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B4188E", "UniRef:UniRef50_UPI0001B4188E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B4188E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B4188E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7743.653400000003, "isoelectric_point": 6.254642295837402 }, "id": "KKNAAMEMLJ_743", "locus": "GCA000027325_00739" }, { "type": "cds", "sequence": "contig_1", "start": 415522, "stop": 415623, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAGAGTAGAAAAATTTCGCTTTTATAGACAAAGTTTTGATAACAATAAGATAGTTAAAAAAGCTTTAATTAACGCTCAGAAGAACACTGAAAGCTGA", "aa": "MGRVEKFRFYRQSFDNNKIVKKALINAQKNTES", "aa_hexdigest": "218d173b86910ef8438da546ab36ae66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3961.5091, "isoelectric_point": 10.282916831970212 }, "id": "KKNAAMEMLJ_744", "locus": "GCA000027325_00740" }, { "type": "cds", "sequence": "contig_1", "start": 415611, "stop": 416330, "strand": "-", "frame": 1, "gene": "tACO1", "product": "putative transcriptional regulatory protein MG332", "db_xrefs": [ "COG:COG0217", "COG:KJ", "GO:0003677", "GO:0005829", "GO:0006355", "RefSeq:WP_009885985.1", "SO:0001217", "UniParc:UPI000013963B", "UniRef:UniRef100_P47574", "UniRef:UniRef50_P47574", "UniRef:UniRef90_P47574" ], "nt": "ATGCCACGTAAGCATCTAATTGCTAATCAAACTAATAAAAAACAACAAACAAGTGCAAAACAACTTCAAAAACTAGCAAAAAGAATAGCTTCAGCTGTTAAAAAAGGTGGAACTAATATCCAGTCAAATCCACATCTAAAAGTTGCAGTTGATCTTGCTTTAGCTAAGGGTCTAAGCATGGATTCAATTAAAAGAAATATCCATGGTAGTGAAAAAGATACAACTAAAATTAGCGAGTTTTGTTATGAGATTTTTGGACCAAATGGTGTTGGAATTATTGTGTTTGGACTAACTGATAATCCTAACCGTTTACTCAGTAGTTTAAACGGTTATTTAGCTAAACTAAAAGGACAATTAGCCAAGCCAAATAGCGTCAAGATTAATTTTCAAGAAGAAGGGATTATCTTTGTTAATAAAAATAACTATTTGAAAGATGATCTAATTGAATTATTAATTTTGGACAATATTAACTTAATTGATGTTGATTATGATGAAGAGTGTTTTGAAATTAGCTTGCATTCAAATAGTTATTTTCATGCAAAGGAGCTGTTGAAAAAAAACAATTTTTCAATAGTAGATAGTGAAATTAAATTGGTACCTCTTTTAACTGTTGATTTAGATAGAAATCAGCAAACTTTATTATCACGTTTTCTCAATGCTTGTGAGGAAGATGATGACATTCAGTTTGTTGTTCATAATGCCAACCCATGGGAAGAGTAG", "aa": "MPRKHLIANQTNKKQQTSAKQLQKLAKRIASAVKKGGTNIQSNPHLKVAVDLALAKGLSMDSIKRNIHGSEKDTTKISEFCYEIFGPNGVGIIVFGLTDNPNRLLSSLNGYLAKLKGQLAKPNSVKINFQEEGIIFVNKNNYLKDDLIELLILDNINLIDVDYDEECFEISLHSNSYFHAKELLKKNNFSIVDSEIKLVPLLTVDLDRNQQTLLSRFLNACEEDDDIQFVVHNANPWEE", "aa_hexdigest": "8eb09ed5c376415173eb966cf3d8aec2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013963B", "ncbi_nrp_id": "WP_009885985.1", "uniref100_id": "UniRef100_P47574", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013963B", "RefSeq:WP_009885985.1", "UniRef:UniRef100_P47574" ] }, "ips": { "uniref100_id": "UniRef100_P47574", "uniref90_id": "UniRef90_P47574" }, "psc": { "uniref90_id": "UniRef90_P47574", "gene": "TACO1", "product": "Probable transcriptional regulatory protein MG332", "uniref50_id": "UniRef50_P47574", "cog_id": "COG0217", "cog_category": "KJ", "go_ids": [ "GO:0005829", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47574", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47574" ], "product": "Probable transcriptional regulatory protein MG332" }, "genes": [ "tACO1" ], "id": "KKNAAMEMLJ_745", "locus": "GCA000027325_00741" }, { "type": "cds", "sequence": "contig_1", "start": 416337, "stop": 416474, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTTTTGCAGGTGTTACACATTTTGAGAGTGTTTTAATTGATGACACTAAAATTCTTTACAAAGATAAAAACAAGCAAGAGGTAGTTGATCTGTTTGCACACAAAGTTGACCAAGTTGCTAATAATTTTTAA", "aa": "MFGFAGVTHFESVLIDDTKILYKDKNKQEVVDLFAHKVDQVANNF", "aa_hexdigest": "82e4ebb11438c9b9f04963f8376f769a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5172.820200000002, "isoelectric_point": 5.393304252624512 }, "id": "KKNAAMEMLJ_746", "locus": "GCA000027325_00742" }, { "type": "cds", "sequence": "contig_1", "start": 416511, "stop": 416675, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02525.22" ], "nt": "ATGACCAACTTTAATTATCCTGCTAGTTTAAAAAACTTTATTGATCATGTCTTTGTAGCTAATAAAACCTTTCAAGACAAATATGTTACTAAAGGTGCTTCTAAGGGATTACTTACTAATCTTAAGGTAGTTGTTTTAGCGTCACAAGGTGCACCATTAGGATGA", "aa": "MTNFNYPASLKNFIDHVFVANKTFQDKYVTKGASKGLLTNLKVVVLASQGAPLG", "aa_hexdigest": "cd4115273f6ad6fa7b62f103ec3e3d2f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02525.22", "name": "Flavodoxin_2", "length": 52, "aa_cov": 0.9629629629629629, "hmm_cov": 0.9807692307692307, "evalue": 3.3187702183034294e-13, "score": 44.84715270996094, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5857.734000000002, "isoelectric_point": 9.778580665588379 }, "id": "KKNAAMEMLJ_747", "locus": "GCA000027325_00743" }, { "type": "cds", "sequence": "contig_1", "start": 416808, "stop": 416930, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAGTTATTATTGTTGATGCTTCTGTAACCCCTTCTGGTTCTTACACCCACCTTTTATTAGATCGTTTCCTTCAAACTTATAAAAAACAAAATAGTAGTGTTGAATTTATTGATTGA", "aa": "MKKVIIVDASVTPSGSYTHLLLDRFLQTYKKQNSSVEFID", "aa_hexdigest": "8f3084ab4a5b37a563b88e61619cbfe8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4573.226500000001, "isoelectric_point": 8.12071170806885 }, "id": "KKNAAMEMLJ_748", "locus": "GCA000027325_00744" }, { "type": "cds", "sequence": "contig_1", "start": 417795, "stop": 418028, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTATTTAACTCCTTTTTTGAAACTAAAAAACTGCCATTTAAAACTGTTTTAATCCACGGTTTAGTACGCGATGAACAAAATCGTAAGATGTCAAAATCACTGAATAATGGCATTGATCCTGTTGATCTTATTAGAAATTATGGAGCGGATGCAGTGCGCTTATTTTTGTGTTCAAATCACACTCCAGGAGATGATCTAATTTTCAGCGAACAAAAAATAAAAAGTGCATGA", "aa": "MLFNSFFETKKLPFKTVLIHGLVRDEQNRKMSKSLNNGIDPVDLIRNYGADAVRLFLCSNHTPGDDLIFSEQKIKSA", "aa_hexdigest": "4ca030f6146eb7eee758fc78ef2bb7eb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001D01CCB6", "query_cov": 0.974025974025974, "subject_cov": 0.8620689655172413, "identity": 0.547, "score": 90.1, "evalue": 4.21e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PEY5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 61, "aa_cov": 0.7922077922077922, "hmm_cov": 0.9836065573770492, "evalue": 3.2944208853669248e-21, "score": 70.54769897460938, "start": 7, "stop": 67 } ], "seq_stats": { "molecular_weight": 8784.024499999996, "isoelectric_point": 8.889432716369633 }, "id": "KKNAAMEMLJ_749", "locus": "GCA000027325_00745" }, { "type": "cds", "sequence": "contig_1", "start": 418500, "stop": 418661, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATGTACTCCTGCACACGACTTTAATGACTATGAAATCAACACTAAATATAAATTTGATTTTCTAAGCTGCATTGACAGTAACGGTATTCTCAATCAAAATGCAAGTAAATTTCAAGGCCTTAGTGTTTTACAAGCAAGAAATAAAATTGTTAAATGA", "aa": "MKCTPAHDFNDYEINTKYKFDFLSCIDSNGILNQNASKFQGLSVLQARNKIVK", "aa_hexdigest": "c4bd564551a58b5abec6317514ccb895", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6069.855600000002, "isoelectric_point": 8.685003471374515 }, "id": "KKNAAMEMLJ_750", "locus": "GCA000027325_00746" }, { "type": "cds", "sequence": "contig_1", "start": 418749, "stop": 418937, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATACTGCTATATCAAATATTGAAGTTATCAATAAACCTGTTAATCAACATCTTCATTATGTTGTTTATAAACTAGCGAATGATAGTAAACAAGAACTAATAGTTGCAACAACAAGACCAGAAACTATCTTTGCTGATGTTTGTCTATTGGTAAACCCAAAAGATAAGCGCTATACTAATTTCTGA", "aa": "MNTAISNIEVINKPVNQHLHYVVYKLANDSKQELIVATTRPETIFADVCLLVNPKDKRYTNF", "aa_hexdigest": "6d270352913e18d0c0d424f6414d3ca7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7132.138000000002, "isoelectric_point": 7.9005521774292005 }, "id": "KKNAAMEMLJ_751", "locus": "GCA000027325_00747" }, { "type": "cds", "sequence": "contig_1", "start": 419470, "stop": 420045, "strand": "-", "frame": 3, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872", "RefSeq:WP_010869438.1", "SO:0001217", "UniParc:UPI000003226F", "UniRef:UniRef100_P47577", "UniRef:UniRef50_P47577", "UniRef:UniRef90_P47577" ], "nt": "ATGGATGCACATTTTTTGAAGAGTGCAAGTGATTTAAAAGATTGTCCTCAAGATAATATCCCAGAGATTTGTTTCATGGGAAGAAGTAATGTTGGTAAATCAAGTTTGATTAATGCTTTTTTTAAAAAGAAACTGGCTAAAACTTCAGCAACACCAGGTCGAACACAATTACTAAATTACTTTGAATATAAAGACAAAAGATTTGTTGATTTACCAGGTTATGGCTTTGCTAAAATAAACAAAAATAAGAAAGATTTTATTACTAATTTACTTACCCAATTTTTAAATTTTCGTAGTAATCTAGTTGGTGTTGTTTTGATAGTAGATAGTGGAGTTGTTACTGTTCAAGATCAGGAAGTGGTTAAAATTATTTTGCAAACAGGATTGAACTTTTTGATTGTTGCTAATAAATTTGACAAACTAAATCAAAGTGAAAGATATTTTTCGCTTAAGAATATTGCAAATTTTTTAAAAGTTAATTTTGATAAATGTGTTTTTGCAAGTACAAAAACACATCATAATCTTGCTTTAGTTCACAAAAAAATTTTCGAATTATTCGTTGAAGATGAAAGATAA", "aa": "MDAHFLKSASDLKDCPQDNIPEICFMGRSNVGKSSLINAFFKKKLAKTSATPGRTQLLNYFEYKDKRFVDLPGYGFAKINKNKKDFITNLLTQFLNFRSNLVGVVLIVDSGVVTVQDQEVVKIILQTGLNFLIVANKFDKLNQSERYFSLKNIANFLKVNFDKCVFASTKTHHNLALVHKKIFELFVEDER", "aa_hexdigest": "9ba36bf1d7661f2035a6696ed6757c31", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000003226F", "ncbi_nrp_id": "WP_010869438.1", "uniref100_id": "UniRef100_P47577", "db_xrefs": [ "SO:0001217", "UniParc:UPI000003226F", "RefSeq:WP_010869438.1", "UniRef:UniRef100_P47577" ] }, "ips": { "uniref100_id": "UniRef100_P47577", "uniref90_id": "UniRef90_P47577" }, "psc": { "uniref90_id": "UniRef90_P47577", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_P47577", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47577" ], "product": "Probable GTP-binding protein EngB" }, "genes": [ "yihA" ], "id": "KKNAAMEMLJ_752", "locus": "GCA000027325_00748" }, { "type": "cds", "sequence": "contig_1", "start": 420053, "stop": 420274, "strand": "-", "frame": 2, "gene": "yneF", "product": "UPF0154 MG335-1-like protein", "db_xrefs": [ "COG:COG3763", "COG:S", "GO:0016020", "KEGG:K09976", "RefSeq:WP_009885966.1", "SO:0001217", "UniParc:UPI0000139652", "UniRef:UniRef100_Q49310", "UniRef:UniRef50_Q9EXD7", "UniRef:UniRef90_Q9EXD7" ], "nt": "ATGAATGATCTAGCACTTGCATTAGGTTTAGGTATCCCTCTTTCGCTTTTGGTGGGAATGATTCTTGGTTATTTTATTTCTATAAAAATCTTTAAAAAACAGATGCGTGACAATCCACCTATTACTGAAAATCAAATTAAAGCAATGTATGCAAAAATGGGTAGAAAATTATCTGAAACCCAGGTAAAAGAGATAATGCGCTCCATTAAAAACCAAAAATAA", "aa": "MNDLALALGLGIPLSLLVGMILGYFISIKIFKKQMRDNPPITENQIKAMYAKMGRKLSETQVKEIMRSIKNQK", "aa_hexdigest": "4ea5b5ae143c256ba0c296af9bf55d2d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139652", "ncbi_nrp_id": "WP_009885966.1", "uniref100_id": "UniRef100_Q49310", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139652", "RefSeq:WP_009885966.1", "UniRef:UniRef100_Q49310" ] }, "ips": { "uniref100_id": "UniRef100_Q49310", "uniref90_id": "UniRef90_Q9EXD7" }, "psc": { "uniref90_id": "UniRef90_Q9EXD7", "gene": "YneF", "product": "UPF0154 protein MG335.1 homolog", "uniref50_id": "UniRef50_Q9EXD7", "cog_id": "COG3763", "cog_category": "S", "kegg_orthology_id": "K09976", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD7" ], "product": "UPF0154 protein MG335.1 homolog" }, "genes": [ "yneF" ], "id": "KKNAAMEMLJ_753", "locus": "GCA000027325_00749" }, { "type": "cds", "sequence": "contig_1", "start": 420333, "stop": 420845, "strand": "+", "frame": 3, "gene": "wcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.1.-", "GO:0005886", "GO:0006071", "GO:0009246", "GO:0016758", "GO:0046467", "GO:0046509", "KEGG:K19004", "SO:0001217", "UniRef:UniRef50_Q9ZB73", "UniRef:UniRef90_Q9ZB73" ], "nt": "ATGGATAAACTTGTTAGTATATTAGTTCCTTGTTACAAATCAAAACCTTTTTTAAAACGTTTTTTTAATTCACTTTTAAAGCAAGATCTTAATCAAGCTAAAATTATTTTTTTCAATGACAATGTTGCTGATGAAACCTATGAAGTTTTGCAAAAATTCAAAAAAGAACACAATAACTTAGCAATTGAAGTCTATTGTGACAAACAGAATGAAGGTATTGGAAAAGTGCGTGATAAGCTAGTGAATCTAGTAACAACACCTTATTTTTATTTTATCGATCCTGATGATTGTTTTAACAACAAAAATGTCATAAAAGAGATTGTTGAATCAATTAAAAAAGAAGATTTTGATCTTGGTGTATTAAAAAGTATGGTCTATTTATGCTTCTTAAAACATGATTTCATTATTAAATTTTTGCCTTTAAAAGGTATTTTTCAAGGCAGAGTAAAATTAATTAATAATAACAATGTTAATAAATTAAATTACATCAAAAATAATGATCAATATATTTGA", "aa": "MDKLVSILVPCYKSKPFLKRFFNSLLKQDLNQAKIIFFNDNVADETYEVLQKFKKEHNNLAIEVYCDKQNEGIGKVRDKLVNLVTTPYFYFIDPDDCFNNKNVIKEIVESIKKEDFDLGVLKSMVYLCFLKHDFIIKFLPLKGIFQGRVKLINNNNVNKLNYIKNNDQYI", "aa_hexdigest": "b0c887849268e8f6998972891a0c9469", "start_type": "ATG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 420333, "stop": 421148, "observations": { "mutation": [ 420843, 420924, 421137 ], "stop": [ 420843, 420924, 421137 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZB73", "query_cov": 1.0, "subject_cov": 0.49853372434017595, "identity": 1.0, "score": 345.0, "evalue": 1.22e-117, "gene_start": 1, "gene_end": 170, "reference_start": 1, "reference_end": 170, "reference_sequence": "MDKLVSILVPCYKSKPFLKRFFNSLLKQDLNQAKIIFFNDNVADETYEVLQKFKKEHNNLAIEVYCDKQNEGIGKVRDKLVNLVTTPYFYFIDPDDCFNNKNVIKEIVESIKKEDFDLGVLKSMVYLCFLKHDFIIKFLPLKGIFQGRVKLINNNNVNKLNYIKNNDQYIWNIVINTDFFRKLNLTFESRLFEDIPIWYPMFFSSQKIVFIDVIGTNYFIRNDSLSTTISAPRYLNLIQCYEKLYVNLSQNGSLASFIDPNHKIEARFWRRQMFVWFALFSFEYFKKNFSESKKILEKLFVFLEKNGVYERVFQTKNQGIYYIWVQRLKYFKHVLESKSDN" }, "paralog": false, "identity": 0.988929889298893, "score": 538.0, "evalue": 2.57e-196, "description": "Internal stop codon at 420843, 420924, 421137. Nonsense mutation around 420843, 420924, 421137.", "psc": { "uniref90_id": "UniRef90_Q9ZB73", "gene": "WcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "ec_ids": [ "2.4.1.-" ], "uniref50_id": "UniRef50_Q9ZB73", "cog_id": "COG0463", "cog_category": "M", "kegg_orthology_id": "K19004", "go_ids": [ "GO:0005886", "GO:0046509", "GO:0016758", "GO:0009246", "GO:0006071", "GO:0046467" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZB73", "EC:2.4.1.-", "UniRef:UniRef50_Q9ZB73", "COG:COG0463", "COG:M", "KEGG:K19004", "GO:0005886", "GO:0046509", "GO:0016758", "GO:0009246", "GO:0006071", "GO:0046467" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB73" ], "product": "Processive diacylglycerol beta-glycosyltransferase" } }, "id": "KKNAAMEMLJ_754", "locus": "GCA000027325_00750" }, { "type": "cds", "sequence": "contig_1", "start": 420933, "stop": 421139, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTTCATCACAAAAAATTGTTTTTATTGATGTGATAGGAACAAATTATTTTATTCGTAATGATAGTTTATCAACTACTATTAGTGCTCCACGCTACTTAAATTTAATCCAATGTTATGAAAAGCTATATGTAAATCTCAGCCAAAATGGTTCTCTTGCAAGTTTTATTGATCCAAATCATAAGATTGAAGCTAGGTTTTGA", "aa": "MFFSSQKIVFIDVIGTNYFIRNDSLSTTISAPRYLNLIQCYEKLYVNLSQNGSLASFIDPNHKIEARF", "aa_hexdigest": "2301ad5b52bd201c850b9dca25639653", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7853.892699999998, "isoelectric_point": 7.8923646926879885 }, "id": "KKNAAMEMLJ_755", "locus": "GCA000027325_00751" }, { "type": "cds", "sequence": "contig_1", "start": 421467, "stop": 421850, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGTCTGCAATTAAATTTAATCCTAGTTCATTCAGAAAAAACTTTAAATGGTTTGAAAATAACAAAAATTGGATTAATTTTGATAATGCTGCTACTTCCATTGCACTTGATGTTGTGGCTGAAGCAAGCAAAGAATATTACCAGTATTTTTGTGTCAATCCTCATAACAAAAATCCTGAAATTAACCAAAAACTTATTGCTATTATTGAAGAAACAAGAGATTTATTAGCAAAATTTTTCAATGCTAAAAAAAATGAAATAATTTTTACAAGTTCTGCAACTGAATCGCTTAACTTATTCGCCTTTGGATTAAGCTCTTTAGTAAAAAGTAATGATGAAATCATTCTCAAAGAAGATGAACATGCTGCTAATGTTTTTCCCTGA", "aa": "MSAIKFNPSSFRKNFKWFENNKNWINFDNAATSIALDVVAEASKEYYQYFCVNPHNKNPEINQKLIAIIEETRDLLAKFFNAKKNEIIFTSSATESLNLFAFGLSSLVKSNDEIILKEDEHAANVFP", "aa_hexdigest": "d2254112d2d3c44a17244c1b4c4922ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 104, "aa_cov": 0.8110236220472441, "hmm_cov": 1.0, "evalue": 3.433536758627499e-27, "score": 90.3916015625, "start": 25, "stop": 127 } ], "seq_stats": { "molecular_weight": 14573.285499999982, "isoelectric_point": 5.6869913101196286 }, "id": "KKNAAMEMLJ_756", "locus": "GCA000027325_00752" }, { "type": "cds", "sequence": "contig_1", "start": 422238, "stop": 422693, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "TTGGATAATAACTTTAAGATAATTTTTAAAGAAGGTCCTTCCAAATTTGAAGCTGGAACGCTAAATATTATGGCTATTTATGCTTTGAATAAACAGTTAAAATTCATGCAAAAAGAATTTAATTTCAGTGAAATGGTGTTTTACAGCAAACAATTAAAAAATTTAGCTTATCAACTGCTAAGTCAAAATCCTAATATCGTTTTAGCTAATCATGATCAAGATGTTCCTATCTTTGCTTTTAAGCATAAATATATTAATTCTGCAGATCTAGCAACTTTTTTAAACATTAAAAAAATAATTGTTAGACAAGGATCCATCTGTGTTGGTAAATTTAAAAATAAAGAGAGTTTTTTACGTGTTTCTCTACTCCATTACAACACAAAAGAGGAATTACTTTATTTAGAAAAATTATTAAAAACTAGTAAGAATTCCATTATTAATGAACTAATATATTAG", "aa": "MDNNFKIIFKEGPSKFEAGTLNIMAIYALNKQLKFMQKEFNFSEMVFYSKQLKNLAYQLLSQNPNIVLANHDQDVPIFAFKHKYINSADLATFLNIKKIIVRQGSICVGKFKNKESFLRVSLLHYNTKEELLYLEKLLKTSKNSIINELIY", "aa_hexdigest": "97b774aef007d732051569d90b9d2d75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 133, "aa_cov": 0.8410596026490066, "hmm_cov": 0.9924812030075187, "evalue": 3.1145061504239515e-30, "score": 100.40299987792969, "start": 7, "stop": 133 } ], "seq_stats": { "molecular_weight": 17636.471600000004, "isoelectric_point": 9.49923915863037 }, "id": "KKNAAMEMLJ_757", "locus": "GCA000027325_00753" }, { "type": "cds", "sequence": "contig_1", "start": 422697, "stop": 423113, "strand": "+", "frame": 3, "gene": "iscU", "product": "putative protein MG337", "db_xrefs": [ "COG:COG0822", "COG:O", "GO:0005506", "GO:0016226", "GO:0051536", "RefSeq:WP_009885968.1", "SO:0001217", "UniParc:UPI0000139647", "UniRef:UniRef100_P47579", "UniRef:UniRef50_P47579", "UniRef:UniRef90_P47579" ], "nt": "ATGGATATTAGAGCAAGAACAAAAATTATTGATATTTATTCAAATTTAAAATACAAAAAACCTCTCAAAAGTTTTCAAAAAATTTTAACAACAAGTGATAGTGATAATTGTGAAGACTTTTTTAATATAGGTTTAAATATTGATAAAAATAAAATTACTGCAATTGGTTTTGATGGTGATGGTTGTATTATCTCTACAATTGCAACTGAATTAAGTATTAAAGCAATTGAAAATAAAACTATTAACCAAGCAAAAAAAATATTATCAGATTTAATTGCAACTTATAAAGATAAAAATTCAGCAAACCAAGTTGTTGAAGAATTAAAGCTTTTAATTGAAATGAATGTAACTGAAAAAAGGTTACAGTGTTTGCTTTTAACACCAAGCAACTTATTGCAATGGTTTAAAAATTTTTAG", "aa": "MDIRARTKIIDIYSNLKYKKPLKSFQKILTTSDSDNCEDFFNIGLNIDKNKITAIGFDGDGCIISTIATELSIKAIENKTINQAKKILSDLIATYKDKNSANQVVEELKLLIEMNVTEKRLQCLLLTPSNLLQWFKNF", "aa_hexdigest": "0dc246878c31421d62ae0f5649a611e9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139647", "ncbi_nrp_id": "WP_009885968.1", "uniref100_id": "UniRef100_P47579", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139647", "RefSeq:WP_009885968.1", "UniRef:UniRef100_P47579" ] }, "ips": { "uniref100_id": "UniRef100_P47579", "uniref90_id": "UniRef90_P47579" }, "psc": { "uniref90_id": "UniRef90_P47579", "gene": "IscU", "product": "Uncharacterized protein MG337", "uniref50_id": "UniRef50_P47579", "cog_id": "COG0822", "cog_category": "O", "go_ids": [ "GO:0005506", "GO:0051536", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_P47579", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47579" ], "product": "Uncharacterized protein MG337" }, "genes": [ "iscU" ], "id": "KKNAAMEMLJ_758", "locus": "GCA000027325_00754" }, { "type": "cds", "sequence": "contig_1", "start": 423100, "stop": 423276, "strand": "-", "frame": 3, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDF155B", "UniRef:UniRef90_UPI002FDF155B" ], "nt": "ATGTCTTTACTTATTGAACAAGCTTTAGATGAAGGCACTCAAGAGTTGGCATTTTTAGATTTAATTCGTGAAAATCAAAAGCAACACCAAAGAGAACATCAACAGATTTTAGTTGGTGATAAACGCTTATATGATATCCTAGGACAAACGTTAGCTATTAATGCTAAAAATTTTTAA", "aa": "MSLLIEQALDEGTQELAFLDLIRENQKQHQREHQQILVGDKRLYDILGQTLAINAKNF", "aa_hexdigest": "94939ef73fd11265fe013b81829fdd69", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 423100, "stop": 423534, "observations": { "mutation": [ 423324 ], "stop": [ 423324 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FDF155B", "query_cov": 1.0, "subject_cov": 0.4027777777777778, "identity": 1.0, "score": 111.0, "evalue": 2.89e-30, "gene_start": 1, "gene_end": 58, "reference_start": 87, "reference_end": 144, "reference_sequence": "MLNVVDRLDDLYFHKFEGYVGLSKAANTDGSNTEGYKTVQTVPLTNDSSIQTLSYVIQITNDDVNKISSNWKNNKANSLGLKKEIFMSLLIEQALDEGTQELAFLDLIRENQKQHQREHQQILVGDKRLYDILGQTLAINAKNF" }, "paralog": false, "identity": 0.9861111111111112, "score": 279.0, "evalue": 5.87e-99, "description": "Internal stop codon at 423324. Nonsense mutation around 423324.", "psc": { "uniref90_id": "UniRef90_UPI002FDF155B", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI002FDF155B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FDF155B", "UniRef:UniRef50_UPI002FDF155B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FDF155B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDF155B" ], "product": "DUF3713 domain-containing protein" } }, "id": "KKNAAMEMLJ_759", "locus": "GCA000027325_00755" }, { "type": "cds", "sequence": "contig_1", "start": 423322, "stop": 423678, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATAAAACCAGTTCTGATGCTGAACTTAATGCTTTTGGTGAGTTATTACATCGCTCAGTTAATGTTGATACAAATAATTTAAGTCGCTTTAACTCTAGAGGTGAACCTTTAATAAGTTTTGATAATAAAAAAAAGTTTTTATTAAATGTAGTTGATAGACTAGATGATCTTTATTTTCATAAATTTGAAGGTTATGTTGGTTTATCTAAAGCTGCAAATACAGATGGTTCCAATACAGAAGGATATAAAACTGTGCAAACAGTTCCATTAACAAATGACTCTAGTATCCAGACTCTTAGTTATGTTATTCAGATAACAAATGATGATGTAAATAAGATTTCTAGTAATTGA", "aa": "MINKTSSDAELNAFGELLHRSVNVDTNNLSRFNSRGEPLISFDNKKKFLLNVVDRLDDLYFHKFEGYVGLSKAANTDGSNTEGYKTVQTVPLTNDSSIQTLSYVIQITNDDVNKISSN", "aa_hexdigest": "40d7784c5e3ec3fe7bbebb82ad286c73", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13179.420299999983, "isoelectric_point": 5.2397253036499025 }, "id": "KKNAAMEMLJ_760", "locus": "GCA000027325_00756" }, { "type": "cds", "sequence": "contig_1", "start": 423682, "stop": 423939, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGAAAGTGAAAACACTTATAGATTTACTGATGAACCGTTTAATAATTCTGTAGCTTTATCTAATAAATCACAAGGATCAAGTGATAAAAAATATTTTTATGGATTTAATGGTTTAACAATTAATTCCAATCAAAGTATTTCAACAGCTAGTGCTGGTTTAACACAACAGCTTTTTAATAATTACGGACAGTTAATTACAGCAACTGATAAAGCAGGCGCTTTAAGTCAATATAAGGATAAGTTTACCTTGTGA", "aa": "MLESENTYRFTDEPFNNSVALSNKSQGSSDKKYFYGFNGLTINSNQSISTASAGLTQQLFNNYGQLITATDKAGALSQYKDKFTL", "aa_hexdigest": "ac5100230865ba6ce0437b357380d75e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9393.166299999992, "isoelectric_point": 6.079976081848144 }, "id": "KKNAAMEMLJ_761", "locus": "GCA000027325_00757" }, { "type": "cds", "sequence": "contig_1", "start": 424192, "stop": 424350, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCAAATGCAAAATAAAGCTGCCCAATCTCATACTTCTAGTAGTGTTAATCAAACCCTATTAGATTTAGCAAAGAAAGCATTTGAAAAGGAATTGGAAGATCCTAATCAGGATGCTGAGTACAAATACATGCGCTTTTTGCAAGCGTTAATGTGA", "aa": "MLQMQNKAAQSHTSSSVNQTLLDLAKKAFEKELEDPNQDAEYKYMRFLQALM", "aa_hexdigest": "76498baee34a784911fae9d47657d9b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6035.8148, "isoelectric_point": 5.6171361923217775 }, "id": "KKNAAMEMLJ_762", "locus": "GCA000027325_00758" }, { "type": "cds", "sequence": "contig_1", "start": 424420, "stop": 424617, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATCTTGATCTTATATTATCACTTTTAACTGATTCAGGAATTAGAACAGGTACTGTTGCAAACATCTTTAAAAATTGGTACTTTAAAAACACTAATTCATTCACTAATAATTTTGATACTTCAAATAAAGAATTAAAAGGTGAATTTTCTGATTTTAATGATCTTGTTAAACAGGCACTTTATATCCGTTCTTGA", "aa": "MDLDLILSLLTDSGIRTGTVANIFKNWYFKNTNSFTNNFDTSNKELKGEFSDFNDLVKQALYIRS", "aa_hexdigest": "9221fa0a0c36ddd2fea0e3142500d2c3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7486.2958, "isoelectric_point": 5.03322925567627 }, "id": "KKNAAMEMLJ_763", "locus": "GCA000027325_00759" }, { "type": "cds", "sequence": "contig_1", "start": 424687, "stop": 425205, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAAATGGAACTTCTAATAATAACGGTCAAAAAGATGTATGTGATTTAGCTAAAAAACTTTTAAAAAACAATACCAATTTATCTGAAACTATTAAAAAACAACAAGATTTTAATAATTCACTTTCACAAATAAAATCAAGTTATGAGTCTTATATTAAAGCTGCTAATAAACTTAATAACTTTAGAACAGATTTAGCTAATATAGAAAAACTAGAACAAGCAATTGTTGATCGTGCTAACAACTACATTAAACTTCAAAAGGAAGCTAAAGAAAGTTCAATTGGTTGGGGTCAACCTCTTCCATATAAAAGAGCAAATGATGGTAGTTATCCTTCATTAGCAAAATTTTTTAATAACAACTCTGATCAAAGTAGTGCTCAAACTTTAACTTTGAAAACCACTGCTGCAGCTATTACTTCTGATAATGAACCAACTCGTGAAAAAAATAATCAAACTTTAAAGACTTTAACAACTGAAGTAGAAAATAAAGCCAAAGAATTAGTTGAAAAATGA", "aa": "MLENGTSNNNGQKDVCDLAKKLLKNNTNLSETIKKQQDFNNSLSQIKSSYESYIKAANKLNNFRTDLANIEKLEQAIVDRANNYIKLQKEAKESSIGWGQPLPYKRANDGSYPSLAKFFNNNSDQSSAQTLTLKTTAAAITSDNEPTREKNNQTLKTLTTEVENKAKELVEK", "aa_hexdigest": "8aeecd8dd1ab99f285420dd9af9bdee1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19280.205400000006, "isoelectric_point": 9.025138664245606 }, "id": "KKNAAMEMLJ_764", "locus": "GCA000027325_00760" }, { "type": "cds", "sequence": "contig_1", "start": 425251, "stop": 425556, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCAAATTCATCTCAAGCACAAGTAGTAACAAGTAATTTATTAGTTAGTCTAAGTTCAAAGACAACTCAAAAACAGCAACAGAAACCTGTTTATGTTAGAGGTGATGATGCCATATATGCATTTCATATTGATGGAGGTAATTATTTTTTAGAGAATTCATCACCTAACAAACGTAATTTTGAAAAACAAGCAGAAGTTTTATTAATGCGATTTTTACAAGGACAAACTAATAATTTCTCTAAAGATAATGTCAGTTTTTCAGTTGATTTATTTGGTTCTAACAGCGAATTTAGAAGTTGA", "aa": "MESNSSQAQVVTSNLLVSLSSKTTQKQQQKPVYVRGDDAIYAFHIDGGNYFLENSSPNKRNFEKQAEVLLMRFLQGQTNNFSKDNVSFSVDLFGSNSEFRS", "aa_hexdigest": "7dbc9403cfa7e941b6890b1c8db80b6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11392.426599999992, "isoelectric_point": 6.553274345397949 }, "id": "KKNAAMEMLJ_765", "locus": "GCA000027325_00761" }, { "type": "cds", "sequence": "contig_1", "start": 425617, "stop": 425757, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAGAGTGCTATGACAAAAAGTCAAGAAGTTACTTCAGACAATAATGGTTTTAATGTTAAAAGTGAATTTTTAAAAATTAATCCCTCGCTTTCTTCTTCTGGTAGTGATAATAGTAGTAACACGCAGTCTTTTTGA", "aa": "MMKSAMTKSQEVTSDNNGFNVKSEFLKINPSLSSSGSDNSSNTQSF", "aa_hexdigest": "6956f923ce8b266dcbd79b6fcabb3f5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4963.360500000002, "isoelectric_point": 5.971186256408691 }, "id": "KKNAAMEMLJ_766", "locus": "GCA000027325_00762" }, { "type": "cds", "sequence": "contig_1", "start": 425936, "stop": 426100, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTTTACTAATTAAAACTTTGCTTGCACTATACTTGTTTTCAAAGTCAAGTAAATCATTTCTGGTATTAGTAGCAGCTGAACTTTGTGATGAACCACTAGAATTAGCAGTTTGTAATTGTTTATCAAACTCTTCAAATAATTTTGCAGAGTTTGTTTGA", "aa": "MLFLLIKTLLALYLFSKSSKSFLVLVAAELCDEPLELAVCNCLSNSSNNFAEFV", "aa_hexdigest": "29d48c29d2fb0bf6049ef0aff8176cfc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5974.032999999998, "isoelectric_point": 4.584314918518066 }, "id": "KKNAAMEMLJ_767", "locus": "GCA000027325_00763" }, { "type": "cds", "sequence": "contig_1", "start": 426262, "stop": 426405, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATTCAAGTGATACAAGCGTTGCAACAACTAAAGAAAACATTGAAAAAACAGAAAATAAACTTAAGTTTATTAACAGCAATTTAAATGATGTAAATGGTGATTTTTTTACTAATCTTCAAGCTTATTTATTTTCACAGTGA", "aa": "MNSSDTSVATTKENIEKTENKLKFINSNLNDVNGDFFTNLQAYLFSQ", "aa_hexdigest": "19d2d05f462909308cc65baeeb6a2ea3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5332.773000000001, "isoelectric_point": 4.593750190734863 }, "id": "KKNAAMEMLJ_768", "locus": "GCA000027325_00764" }, { "type": "cds", "sequence": "contig_1", "start": 426658, "stop": 426861, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTGCAACAGCAATAATTACAGCCTGTTCCACAATAAACACTGCTAATCTTTTTCCTACTCTCAATCGATCCCAAAAACTAATTGGTTTTAGTGATCAAAATATCATAAACCCAGATGTAGTTTTAAAGTCTGCTTTAAATGATGGTAGTGGTGTTGATTCTATCTTACGTGTTAGTTTTGGATCAGCATTACAAAAATGA", "aa": "MGATAIITACSTINTANLFPTLNRSQKLIGFSDQNIINPDVVLKSALNDGSGVDSILRVSFGSALQK", "aa_hexdigest": "8d840155f7d4cb0b7eb1aebb2784c033", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7008.939300000002, "isoelectric_point": 7.934011268615723 }, "id": "KKNAAMEMLJ_769", "locus": "GCA000027325_00765" }, { "type": "cds", "sequence": "contig_1", "start": 427090, "stop": 428112, "strand": "-", "frame": 3, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "COG:COG0468", "COG:L", "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664", "KEGG:K03553", "RefSeq:WP_009885970.1", "SO:0001217", "UniParc:UPI00001334FA", "UniRef:UniRef100_P47581", "UniRef:UniRef50_P78014", "UniRef:UniRef90_P47581" ], "nt": "ATGGCTCAAAAAGAAATAATTAATAAGAAAAATACTCAAAAAAATAGTAGTTTTATTGAAAGTAATAATTTGACAAGTTTTGATTTTTTTGATGCAAAGAAAAACAGTGAAATTGAAACAATTTCAACTGGAAGTTTAAATTTAGATGAAGCATTAGGGTCTGGTGGTCTACCTTTAGGTAGGATAGTAGAACTATATGGAAATGAATCATCTGGAAAAACAACTATTGCACTAAATGCAGTCGCTAGTTTTCAGAAAGCAGGTAAAACAGCATGTTATATTGATGCTGAAGGTGCACTTGATTTAGCATATGCTAAATCAATTGGTATTGATCTAAATAAACTTTTGATTGCTCATCCTAGGCATGGTGAAAACGCTTTTGCTCTTATCGAATCATTAATTAAAACAAACAAGATATCTTTAATTGTTATTGACTCTGTAGCAGCGTTAATTCCTAAACAAGAGTTAGAAGGCACAATTGAAGAACAAACTATTGGCTTGCATGCAAGAATGATGTCAAAAGGTTTGCGAAGAATACAATCGATATTACCAGATTCTAAAACTTGTGTTTTATTCATTAATCAGTTACGCGAAAAACCAGGAGTGATGTTTGGAAATAACGAAGTTACAACAGGAGGAAAAGCTCTAAGATTTTATAGTTCATTAAGAATGGAAGCTAAGCGTGTTGAATTACTTAAGGATAAATTCAACAATTATGTTGGCATAAAAACAAAAGTAATGGTATCTAAAAATAAGATTGCTAAACCTTTTGGTGTTGCTATATTAGAAATCATGTTTAACCGTGGTTTTGTACATGAACATGAAGTTATTGACTTAGCACTTAAATTTAATGTTGTTGTAAGAGCTGGAAATTCTTATTCTTTTAACAATGAAAGCATTGCTGTTGGTAAAGAAAAATTATTAAATGTTTTATCAGAAAAGCCAGCATTATTTGAACAAATAAAAGAACTAACTGTTCAACAATTGGCTAATAAAAATTCATTTCAACAAACAGCTAGCTAA", "aa": "MAQKEIINKKNTQKNSSFIESNNLTSFDFFDAKKNSEIETISTGSLNLDEALGSGGLPLGRIVELYGNESSGKTTIALNAVASFQKAGKTACYIDAEGALDLAYAKSIGIDLNKLLIAHPRHGENAFALIESLIKTNKISLIVIDSVAALIPKQELEGTIEEQTIGLHARMMSKGLRRIQSILPDSKTCVLFINQLREKPGVMFGNNEVTTGGKALRFYSSLRMEAKRVELLKDKFNNYVGIKTKVMVSKNKIAKPFGVAILEIMFNRGFVHEHEVIDLALKFNVVVRAGNSYSFNNESIAVGKEKLLNVLSEKPALFEQIKELTVQQLANKNSFQQTAS", "aa_hexdigest": "de552d34d44dec5d67f423ae90a06d72", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001334FA", "ncbi_nrp_id": "WP_009885970.1", "uniref100_id": "UniRef100_P47581", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001334FA", "RefSeq:WP_009885970.1", "UniRef:UniRef100_P47581" ] }, "ips": { "uniref100_id": "UniRef100_P47581", "uniref90_id": "UniRef90_P47581" }, "psc": { "uniref90_id": "UniRef90_P47581", "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_P78014", "cog_id": "COG0468", "cog_category": "L", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664" ] }, "pscc": { "uniref50_id": "UniRef50_P78014", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78014" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "KKNAAMEMLJ_770", "locus": "GCA000027325_00766" }, { "type": "cds", "sequence": "contig_1", "start": 428454, "stop": 428627, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "ATGGTGAAAAAGTATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAATATACCTTAAACAGTAGTTTGGCCAAACAAAAGGGCAAAAGCCAGATAGAGGTACATCTTGGTTCAAATTCAAATCAGTGA", "aa": "MVKKYQLDVPNVSSNMNQVLQELILEQPLTKYTLNSSLAKQKGKSQIEVHLGSNSNQ", "aa_hexdigest": "e9aa813ca5b821c7f9e5c38f859b0737", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 428325, "stop": 428692, "observations": { "deletion": [ 428625 ], "mutation": [ 428448 ], "stop": [ 428448 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 0.9298245614035088, "subject_cov": 0.4380165289256198, "identity": 0.943, "score": 97.1, "evalue": 8.64e-25, "gene_start": 1, "gene_end": 53, "reference_start": 42, "reference_end": 94, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7398373983739838, "score": 166.0, "evalue": 7.67e-55, "description": "Internal stop codon at 428448. Frameshift due to deletion around 428625. Nonsense mutation around 428448.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6415.267000000002, "isoelectric_point": 9.158652687072756 }, "id": "KKNAAMEMLJ_771", "locus": "GCA000027325_00767" }, { "type": "cds", "sequence": "contig_1", "start": 429159, "stop": 429359, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "TTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGCTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGA", "aa": "MGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQIIFSGFKAGSVGYDSSSSSSSSSSTKDQALA", "aa_hexdigest": "2cc593df1a42b541ced6bffe665bb9e4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 68, "aa_cov": 1.0, "hmm_cov": 0.9705882352941176, "evalue": 3.059693225364716e-19, "score": 64.30823516845703, "start": 1, "stop": 66 } ], "seq_stats": { "molecular_weight": 6947.354700000003, "isoelectric_point": 5.974028205871583 }, "id": "KKNAAMEMLJ_772", "locus": "GCA000027325_00768" }, { "type": "cds", "sequence": "contig_1", "start": 429405, "stop": 429662, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "GTGACCAACGACACGGGATTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCTCATTCGTTGTTCCTTATTCGGGGAATCATAGTAATCAAATTTCATCAGGAACCATTAAAACTGCTTATCCAGTGAAAAAAGATGAAGCTTCCCAAGTAGCGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGAAGTGGAATCTTCTTCCTCTTTCAACCCCACCCAAAGGTTGCAAAAAGATAG", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSGNHSNQISSGTIKTAYPVKKDEASQVAINSLINATPLNSYGSGIFFLFQPHPKVAKR", "aa_hexdigest": "cc6ddbfbd77176b13c7eef933ff9c5c6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 68, "aa_cov": 0.8, "hmm_cov": 1.0, "evalue": 2.814387254053358e-21, "score": 70.96951293945312, "start": 3, "stop": 70 } ], "seq_stats": { "molecular_weight": 9135.115399999997, "isoelectric_point": 9.15826587677002 }, "id": "KKNAAMEMLJ_773", "locus": "GCA000027325_00769" }, { "type": "cds", "sequence": "contig_1", "start": 429607, "stop": 429900, "strand": "+", "frame": 1, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2", "UniRef:UniRef90_UPI001EE6771E" ], "nt": "ATGGAAGTGGAATCTTCTTCCTCTTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCGGTGAAGGATTCAAACAAAGACAGTGAGAAACTCAGTGAAACAACTGCTTCATCCATGAGTGGTATGGCTACATCTCCTCGCAAAGCCCTCAAAGTGGAGGTGAAAAAACAAAGTGGATCAAGTGACACCCTCACCAAAAACGACTTTGCTAAAAAGCCACTGCAGCATAAGAACAGTAGTGGGACAGAGGTGAAGTTAGATGCGAGTGGGGAGTTTGGTGACAACAAAGCCTGA", "aa": "MEVESSSSFNPTQRLQKDSPVKDSNKDSEKLSETTASSMSGMATSPRKALKVEVKKQSGSSDTLTKNDFAKKPLQHKNSSGTEVKLDASGEFGDNKA", "aa_hexdigest": "a721469546af9a4541042ec5166b8434", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 429607, "stop": 430116, "observations": { "mutation": [ 429898, 430099 ], "stop": [ 429898, 430099 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EE6771E", "query_cov": 1.0, "subject_cov": 0.5739644970414202, "identity": 1.0, "score": 173.0, "evalue": 1.03e-53, "gene_start": 1, "gene_end": 97, "reference_start": 1, "reference_end": 97, "reference_sequence": "MEVESSSSFNPTQRLQKDSPVKDSNKDSEKLSETTASSMSGMATSPRKALKVEVKKQSGSSDTLTKNDFAKKPLQHKNSSGTEVKLDASGEFGDNKAWKPLLTTEQIAREKRMGMPTPPPLLLLLLLLLLLLLLLLLLLLLLLLLLLPQPPSPLFLTSMLGLNQWSLNI" }, "paralog": false, "identity": 0.9881656804733728, "score": 256.0, "evalue": 5.19e-89, "description": "Internal stop codon at 429898, 430099. Nonsense mutation around 429898, 430099.", "psc": { "uniref90_id": "UniRef90_UPI001EE6771E", "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EE6771E", "UniRef:UniRef50_A0A7M3VDH2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2" ], "product": "MgpB adhesin protein" } }, "id": "KKNAAMEMLJ_774", "locus": "GCA000027325_00770" }, { "type": "cds", "sequence": "contig_1", "start": 429914, "stop": 430105, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_332897093.1", "SO:0001217", "UniParc:UPI0003200FB3", "UniRef:UniRef100_UPI0003200FB3", "UniRef:UniRef50_UPI0003200FB3", "UniRef:UniRef90_UPI0003200FB3" ], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGGGGGGTTGAGGAAGTAGAAGTAGTAGTAGTAGTAGTAGTAGTAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGGGAGGGGGAGTTGGCATCCCCATCCTCTTCTCTCTTGCTATTTGCTCGGTAG", "aa": "MIIDLTPTLMLEKVGRGVEEVEVVVVVVVVEEEEEEEEEEEEEEEEGEGELASPSSSLLLFAR", "aa_hexdigest": "8c6b4894fe151a009afaa970700ca28d", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003200FB3", "ncbi_nrp_id": "WP_332897093.1", "uniref100_id": "UniRef100_UPI0003200FB3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003200FB3", "RefSeq:WP_332897093.1", "UniRef:UniRef100_UPI0003200FB3" ] }, "ips": { "uniref100_id": "UniRef100_UPI0003200FB3", "uniref90_id": "UniRef90_UPI0003200FB3" }, "psc": { "uniref90_id": "UniRef90_UPI0003200FB3", "uniref50_id": "UniRef50_UPI0003200FB3" }, "pscc": { "uniref50_id": "UniRef50_UPI0003200FB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003200FB3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7047.687999999992, "isoelectric_point": 4.0500284194946286 }, "id": "KKNAAMEMLJ_775", "locus": "GCA000027325_00771" }, { "type": "cds", "sequence": "contig_1", "start": 430368, "stop": 430556, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCE5FDA", "UniRef:UniRef90_UPI002FCE5FDA" ], "nt": "TTGACTTTCACTAACAAGAATAATCCGCAGCGCAATCAACTGTTGCTCAGAAGCTTACTAGGAACTATCCCGGTCTTGATCAATAAGAGTGGAACGGGAGATGAGTTTAACCATACGAGTGAGCAGAAGTGGGATAAAACTGAGACCAAGGAAGGCAACCTCCCAGGGTTTGGGGAAGTGAATGGGTGA", "aa": "MTFTNKNNPQRNQLLLRSLLGTIPVLINKSGTGDEFNHTSEQKWDKTETKEGNLPGFGEVNG", "aa_hexdigest": "857f2059d8b13c9379accb5cfeb0aaeb", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 430137, "stop": 430583, "observations": { "mutation": [ 430167, 430356, 430554 ], "stop": [ 430167, 430356, 430554 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE5FDA", "query_cov": 1.0, "subject_cov": 0.44285714285714284, "identity": 0.919, "score": 122.0, "evalue": 1.19e-34, "gene_start": 1, "gene_end": 62, "reference_start": 70, "reference_end": 131, "reference_sequence": "MFIPNSSPDIWTGAGYRKGNDTNGIPFDQVKPSSPSFDPNSSDNKVNSKPTTYSFLPNSISPTSDWINALTFTNKNNPQRNQLLLRGLLGTIPVLINKSGTGDEFTHTNDQKWDKTETKEGNLPGFGEVNGWKIVTIFLN" }, "paralog": false, "identity": 0.7905405405405406, "score": 224.0, "evalue": 3.19e-77, "description": "Internal stop codon at 430167, 430356, 430554. Nonsense mutation around 430167, 430356, 430554.", "psc": { "uniref90_id": "UniRef90_UPI002FCE5FDA", "product": "adhesin", "uniref50_id": "UniRef50_UPI002FCE5FDA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE5FDA", "UniRef:UniRef50_UPI002FCE5FDA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FCE5FDA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCE5FDA" ], "product": "adhesin" } }, "id": "KKNAAMEMLJ_776", "locus": "GCA000027325_00772" }, { "type": "cds", "sequence": "contig_1", "start": 430580, "stop": 432103, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "GTGAAAGAAAAAGTATCTGATGAGATCCAAAACTTAATTAAACAAGAACAGTACCGTGATAATTCCATTGTGGTAATGGCTGATTCAGGTGCTAGATGTAACATTTCTAACTTTACCCAGTTATTTGGGATGCGAGGCTTAATGTCTAAAAGCTTTAACTATGAAAGAAATAACCAATCTAAGATCATTAAAGATACGATAGAAGTTCCTATTAAACACTCCTTTTTTGAAGGTTTGACCATTAATGAATACTTCAACTCTTCTTATGGAGCGAGAAAAGGGATGACAGATACTGCAATGAAAACAGCAAAGTCTGGTTATATGACAAGAAAGCTAGTAGATGCTACTCATGAATTAATTATTAACCATGATGATTGTGGAACAAGAAAAGGAATTGTTGTTGAAGCAATTGTTGAAACCAAAACCAAATCCTTGATTGAATCATTATTTGACAGGATTGTTAATCGCTACTCAATTACTCCTATAGTTGATCCTGAAACACAAAAAACTATTGTAGAAGCTAACAGTCTTATTACAACGCAATTAGCTAAACAGATTTGTGCAACATCTATTAAAGAAGTTTTAGTTAGATCTGTTATCTATTGTGAAAGGGAAAATGGTATCTGTCAATACTGCTTTGGCATTGACTTGTCAACTGGTAAGTTGGTGGAATTGGGAACTGCTGTTGGGGTGATTGCTGCTCAATCAATTGGTGAACCTGGGACCCAATTGACAATGCGTACTTTCCATACTGGGGGAGTTTCAACTGAAAACAACTTAGCACAAGGCTTTGAACGTTTAAAACAGATCTTTGAAGTAGTTACTCCTAAAGATTTTGAAAAAGCAGTTATCTCTGAAGTGAAAGGAACAGTTAAATCAATTACTACTGTTCAAAACGCTCAGGAAGTAGTGATTAAATCAAACGTTGATGAGAGGATTTATACTATCCCTTTCAGTGCTCAAATACGTGTTCATGTTGGTGATCAAGTTTCACCAGGTTCTAAGATTACAGAAGGTTCTGTTGATATTAAACAACTTTTGCGAATTGCAGGTATCCAAAGGGTAAGACAATACATGATTGTAGAGATCCAAAAAGTGTATAGGATTCAAGGGATAGATATTGCTGATAAGTATGTAGAAATTATTATCAGACAACTAACTAATTTGTTGCAAGTAACAGATGCAGGTAACAGCAATTTATTTGTTGGTCAGTTAGTGCATAGCCATTATCTCAATGAACTAAATAAGAGCTTACTTTTAGCTGGAAAGATGCCTGTTATTGCAATTAATCAGGTGTTTGGAATTGATGAAGCGGCAAGTAAATCTAACTCCTTTTTAAGTGCTGCATCCTTCCAAGATACCAAAAAAATCCTAACTGATGCTGCTGTTAAGAACCAAGTAGACTATCTTTTAGGTTTAAAAGAGAATGTTATTATTGGTGGAAAAATTCCAGCAGGAACAGGATTTTTAACTGATGAAGAGTTAACTTTCTTAGGTAGCAAAACAGTTGCTGAAGAGTATTAA", "aa": "MKEKVSDEIQNLIKQEQYRDNSIVVMADSGARCNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFFEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTKSLIESLFDRIVNRYSITPIVDPETQKTIVEANSLITTQLAKQICATSIKEVLVRSVIYCERENGICQYCFGIDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVTPKDFEKAVISEVKGTVKSITTVQNAQEVVIKSNVDERIYTIPFSAQIRVHVGDQVSPGSKITEGSVDIKQLLRIAGIQRVRQYMIVEIQKVYRIQGIDIADKYVEIIIRQLTNLLQVTDAGNSNLFVGQLVHSHYLNELNKSLLLAGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKNQVDYLLGLKENVIIGGKIPAGTGFLTDEELTFLGSKTVAEEY", "aa_hexdigest": "f7e3aefd754ef7e1500b8d418f8d1e5d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 78, "aa_cov": 0.14595660749506903, "hmm_cov": 0.9871794871794872, "evalue": 2.2267448334140948e-17, "score": 58.036529541015625, "start": 2, "stop": 75 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 339, "aa_cov": 0.6331360946745562, "hmm_cov": 0.7581120943952803, "evalue": 1.690978068386074e-58, "score": 193.2397918701172, "start": 78, "stop": 398 } ], "seq_stats": { "molecular_weight": 56151.773200000054, "isoelectric_point": 6.6935529708862305 }, "id": "KKNAAMEMLJ_777", "locus": "GCA000027325_00773" }, { "type": "cds", "sequence": "contig_1", "start": 432110, "stop": 434155, "strand": "-", "frame": 2, "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "db_xrefs": [ "COG:COG0086", "COG:K", "EC:2.7.7.6", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270", "KEGG:K03046", "SO:0001217", "UniRef:UniRef50_P47582", "UniRef:UniRef90_P47582" ], "nt": "ATGGGACATATTGCACTTGTGAGTCCTGTAGCTCACATTTGGATGTCAAAAGAATTACCATCTCCTTCCAAAATATCATTAGTTTTAAACATCTCTTACAAAGAGGTTGAACAGGTTTTGTACTTTGTTAACTACATAGTACTTGATACAGGTAAGATCAAAGATGATAAAATCATGCCTTTTAAGTTCAAAGAAGTTTTGGACTTAACTGGTAAGGGTTCACTTTCAACACGACAAAAAATGCGTCGTGTGATAGGTTATATCTTCAGAAATCTCATTAAAAGTAAGAGTAGTGAAGATTACCGTAAGGGAAAAATCTTTTATGAAAGTTTAAAAAACAGCTCTCTCCCCTTTTCTCTAAATGATGCTTTTAATTACATTAAGAAGTACACTGGTTTTAGGGTTGGAATAGGGGCTGAAGCAATTTTGGAATTGCTTAATAAAATCGATCTTAACTTGGAATTTAGCAGGTTAAATGATGCTTTAAGAAAAGCCAAGAAAGATAGTGTTGAAGATGCTAAAGTTAAGAAGATCTTAAGACAACTGGAAACTATTAGTTGGTTTAGAAATTCTAAGCTTCATCCTAAAAACATGATCTTACATACTGTTCCAGTTATCCCCCCTGATATCAGACCTATTATCCAACTTGATGGTGCTAAGTTTACCACCAGTGACATCAACAATTTTTACCGCAGGGTAATCATTAGAAATGACCGATTAAGAAGGATTTTGGAAGATGGTACTGTACCTTCTATTGTTGTTAACAATGAAAAAAGACTTTTACAAGAGTCTGTTGATGCTTTATTTGATAACTCTTCACGTCATAAACCATCACTTTCCAAAGACAAACGGTCATTGAAATCTTTAACAGATCGTTTAAAAGGAAAACAAGGTTTATTTAGACACAACTTACTTGGTAAAAGAGTTGATTATTCAGGTAGAAGTGTAATTGTGGTTGGCCCTGAATTGAAGATGTATGAAGTTGGGATCCCAGCACTAATGATCTTAAAGCTGTTTAAACCCTTTATTATCCATGGATTGATCAATAAGTTTGATGAAAATGGTAATGAGATTAGACCAATTGCCGCTTCCATCAGACAAGCTGAAGATATGATTAAAAACCAGGATGATCTTATCTGGGGAATAGTTTATGATGTTATCAAAGATCGTCCCGTTTTACTAAATCGTGCTCCAACCCTACATAGGTTAGGGATCCAAGCATTTGAACCAAGAATTGTTGATGGTAAAGCAATTAGATTACACCCATTAGTAACTACTGCATTTAATGCTGATTTTGATGGTGATCAGATGGCAGTTCATGTTCCTTTAAGTGAGAATGCAGTTAATGAAGCAAGAGCTGTTCTGCTTGCATCAAAACATATCTTAGGTTTGAAAGATGGAAGACCTATTGTAACTCCTACTCAGGACATGGTTTTAGGTAACTATTATCTAACCACAGAGAGAAAAGGACAGTTGGGAGAGGGGATTATCTTCAGCACAGTTTATGAAGCACGTGCTGCTTATGAAAGTCAAAAGGTTCATTTACATGCTATTGTAGGGATAAGTACTAAAGCATTTCCCAACAAGAAGTTTGCATGCCAAGGAACTTTAATAACAACAGTTGGTAAGATTATCTTTAATGATGTTTTAGGCAATAATGTTCCTTATATTAATGACGGGGAATTTGATGAAAATGCATGTCCCGAAAAGTTCATTGTGAAACAGGGAGAAGATGTAAGACAATCAATTTTAAAGCATCAAATTATCCCTGCATTTTCCAAAAAGGTTATTTCCAAGTTAATCGATCTACTCTATCTTTTATTGGAATTTAAAGACCTTCCTAAAACACTTGATAATATCAAAGCACTTGGCTTTAAGTACTCTACTTTTTCTTCAACTACTGTTTCAGTATTTGATATCCCTAAGTACACCAATAAACAAAATTACTTTGATAGTGCTGATCAACAGGTGCTGAAATACAAACAGTTTTATAACAAGGGTTTGTTAACCGATGATGAACGTTATAAACGCGTTGTGAAGTTATGA", "aa": "MGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDDKIMPFKFKEVLDLTGKGSLSTRQKMRRVIGYIFRNLIKSKSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNLEFSRLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPSIVVNNEKRLLQESVDALFDNSSRHKPSLSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDENGNEIRPIAASIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAVLLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQLGEGIIFSTVYEARAAYESQKVHLHAIVGISTKAFPNKKFACQGTLITTVGKIIFNDVLGNNVPYINDGEFDENACPEKFIVKQGEDVRQSILKHQIIPAFSKKVISKLIDLLYLLLEFKDLPKTLDNIKALGFKYSTFSSTTVSVFDIPKYTNKQNYFDSADQQVLKYKQFYNKGLLTDDERYKRVVKL", "aa_hexdigest": "92b91b1eb9ecd9e3efc01938141ad06c", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoC" ], "truncated": "both", "pseudogene": { "start": 431807, "stop": 434455, "observations": { "mutation": [ 432112, 434188, 434362 ], "stop": [ 432112, 434188, 434362 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47582", "query_cov": 1.0, "subject_cov": 0.5270897832817337, "identity": 1.0, "score": 1324.0, "evalue": 0.0, "gene_start": 1, "gene_end": 681, "reference_start": 102, "reference_end": 782, "reference_sequence": "MTTTRRNKRNNKLYKNIKAIKLSIASNDTILNWSEGEVTKAETINYKSLKPEPGGLFDEAIFGPVKDYECACGKFKKIKYRGVRCDRCGVWVTESIVRRERMGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDDKIMPFKFKEVLDLTGKGSLSTRQKMRRVIGYIFRNLIKSKSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNLEFSRLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPSIVVNNEKRLLQESVDALFDNSSRHKPSLSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDENGNEIRPIAASIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAVLLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQLGEGIIFSTVYEARAAYESQKVHLHAIVGISTKAFPNKKFACQGTLITTVGKIIFNDVLGNNVPYINDGEFDENACPEKFIVKQGEDVRQSILKHQIIPAFSKKVISKLIDLLYLLLEFKDLPKTLDNIKALGFKYSTFSSTTVSVFDIPKYTNKQNYFDSADQQVLKYKQFYNKGLLTDDERYKRVVKLWNNVKEKVSDEIQNLIKQEQYRDNSIVVMADSGARCNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFFEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTKSLIESLFDRIVNRYSITPIVDPETQKTIVEANSLITTQLAKQICATSIKEVLVRSVIYCERENGICQYCFGIDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVTPKDFEKAVISEVKGTVKSITTVQNAQEVVIKSNVDERIYTIPFSAQIRVHVGDQVSPGSKITEGSVDIKQLLRIAGIQRVRQYMIVEIQKVYRIQGIDIADKYVEIIIRQLTNLLQVTDAGNSNLFVGQLVHSHYLNELNKSLLLAGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKNQVDYLLGLKENVIIGGKIPAGTGFLTDEELTFLGSKTVAEEY" }, "paralog": false, "identity": 0.9965986394557823, "score": 1731.0, "evalue": 0.0, "description": "Internal stop codon at 432112, 434188, 434362. Nonsense mutation around 432112, 434188, 434362.", "psc": { "uniref90_id": "UniRef90_P47582", "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47582", "cog_id": "COG0086", "cog_category": "K", "kegg_orthology_id": "K03046", "go_ids": [ "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47582", "EC:2.7.7.6", "UniRef:UniRef50_P47582", "COG:COG0086", "COG:K", "KEGG:K03046", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47582", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47582" ], "product": "DNA-directed RNA polymerase subunit beta'" } }, "id": "KKNAAMEMLJ_778", "locus": "GCA000027325_00774" }, { "type": "cds", "sequence": "contig_1", "start": 434360, "stop": 434458, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAACAACAAGACGTAATAAAAGAAATAACAAGCTTTATAAAAACATTAAAGCAATTAAACTTTCCATCGCTTCCAATGACACCATTTTGAACTGA", "aa": "MTTTRRNKRNNKLYKNIKAIKLSIASNDTILN", "aa_hexdigest": "05503160a2613d897970a8709258a958", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3734.3353000000006, "isoelectric_point": 11.165940284729004 }, "id": "KKNAAMEMLJ_779", "locus": "GCA000027325_00775" }, { "type": "cds", "sequence": "contig_1", "start": 434468, "stop": 434737, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04560.25" ], "nt": "TTGCAAGAACTTTTAACCATTAAATCTGATGATGTACAAGGAAGAAATAGGGCTTATGCTGCTATTGTTAAAGGTGCAGCTTTCCCAGAGCCTGGTATCCCTGAATCATTTAAATTATTGACAAAAGAATTACAGGGCTTGGCTTTATCTGTTTCATTTATCTATGATGACAACACCCAACAAGACTCCAATAATGTTTCCATCTTGCAAAGTGATGGGGAACAAGATGAATTTTTCAATGATTTTGAATTTGACACTGAGGGTTATTAG", "aa": "MQELLTIKSDDVQGRNRAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQSDGEQDEFFNDFEFDTEGY", "aa_hexdigest": "7ad708e8f50a0819af35d6c2caab9c68", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04560.25", "name": "RNA_pol_Rpb2_7", "length": 64, "aa_cov": 0.6067415730337079, "hmm_cov": 1.0, "evalue": 3.4693155402357114e-13, "score": 44.94830322265625, "start": 1, "stop": 54 } ], "seq_stats": { "molecular_weight": 9971.774399999997, "isoelectric_point": 4.0500284194946286 }, "id": "KKNAAMEMLJ_780", "locus": "GCA000027325_00776" }, { "type": "cds", "sequence": "contig_1", "start": 434768, "stop": 435844, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "TTGTCTACTAAAAATGGTGATGAACAAATTACCCGTGATATCCCTAATGTTAGTGATGCAAACAAACGCTATCTTGATGAGAATGGCATCATTATGGTGGGTGCTGAAGTTAAAGAAGGGGATGTTTTGGTTGGTAAAGTTTCCCCTAAAGGTCAAGTCGAAGTCTCTCCTGAAGAAAAGCTATTTAAAGCCATCTTCCCTGAAAGTGTTCAAAACGTGAGAGACTCTTCACTTAAAGTTTCCCATGGTGGGGATGGTATTGTTTCAGCTGTAAAACGTTTTTCAATTGCTAATGGTGATGAACTTAATGATGGTGTGATTGAAATGATCAAGGTTTATGTGGTTCAAAAACGTAAGATTCAAATTGGTGATAAATTAGCTGGTAGACACGGAAATAAAGGAGTTATTTCTAAAGTGGTGCCTATTGAAGATATGCCCCATTTAGAAGATGGAACCCCAGTTGATATTCTGCTCAACCCCCTTGGTGTTCCTAGTCGGATGAACATAGGACAAATTTTTGAAACCCACTTGGGTTATGCAGCACACAAGCTAGCAGTTCGTTCTTTAATTAGTAGTTGTTTTGATCAAAATAAAGCTAAGGAGTTTGCCATTGAAATCAATCAACCTCAAGCAAGGGTTGAAAGATTAATTAAAGGTTTAAAAAACCAAATCAATGATCGCAATATTAAAAGTGAAAAAGAAGCACTTGAAAAACTCGATAACAGTGACATTAGTTTAGTTTTGAAAGAGATAGGGATGTCTTTTGATGATCTTATTTACAAAATTGCAACCCCTATTTTCCAAGGAGTGAACTTCTTAGATCTCCAAGATGTTATGCAAGAAGCAGGATTAGATCCCCAAAAAAATCAGGGTAAGTTTAAACTCATTGATGGTAGGAGTGGAATGCCATTTGAAAGACCTATTTCACTTGGAATTATGTACATGATGAAGCTGAATCACATGGTTGATGATAAGATCCATGCTCGTGCTGTTGGCCCTTATTCTAAGATCACTCAACAACCATTAGGTGGTAAATCGCAAAATGGTGGACAGCGGTTTGGTGAGATGGAAGTGTGA", "aa": "MSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKVSHGGDGIVSAVKRFSIANGDELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPIEDMPHLEDGTPVDILLNPLGVPSRMNIGQIFETHLGYAAHKLAVRSLISSCFDQNKAKEFAIEINQPQARVERLIKGLKNQINDRNIKSEKEALEKLDNSDISLVLKEIGMSFDDLIYKIATPIFQGVNFLDLQDVMQEAGLDPQKNQGKFKLIDGRSGMPFERPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEV", "aa_hexdigest": "4b6f0dffe8a81e6dd3363169bc072d80", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 351, "aa_cov": 0.9636871508379888, "hmm_cov": 0.7435897435897436, "evalue": 5.491459755297721e-95, "score": 313.65203857421875, "start": 3, "stop": 347 } ], "seq_stats": { "molecular_weight": 39573.02310000004, "isoelectric_point": 6.421294212341309 }, "id": "KKNAAMEMLJ_781", "locus": "GCA000027325_00777" }, { "type": "cds", "sequence": "contig_1", "start": 435944, "stop": 438640, "strand": "-", "frame": 2, "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "COG:COG0085", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "KEGG:K03043", "SO:0001217", "UniRef:UniRef50_P47715", "UniRef:UniRef90_P47583" ], "nt": "ATGTCACAAAAATCTAATTTCTTTCAAAAACGTTATTCCCCTACAGCTACCAGAAGGTATTATGGCAAAATTGAGACCAATTTTATCCAACCAAATTTAGCTGATATTCAGATTAAAAGCTACCAAAAATTCTTAGATCATGATCTTGAAAAATTAATTGCCTCATATTTTCCAATCAAATCCCCTAATGATCGCTACACTATCAATTTTAGGGGATTACACAGAACTGAACCAGAACGTGATGAAGCACAATCACGTGCTCAATCTAAAACTTATGAAGTTGGTATTTATGCTGATCTTGAATTAGTTGATAATGATAAAGGAACAGTTAAAAAAGCACGGAAATCAAAGAAAAATATTGCTAGCAATACAAATGGTGTATTTTTAGCTAGCATGCCTTTAATAACCCATGATGGGGTTTTTATTATCAATGGGATAGAAAAGTTTGTTATTTCCCAAATAACCCGTTCTCCAGGGATATACATGCTAACAAAATCCCAACTAAAACTATCCAACTCCCGTAAAAGAGTACAGGAAGGTTATGTTTGTGAGGTTTTACCTGCTAATGGTTCAGTGATGCTTATATACATCTCCAATAAAAAAAAGATTGAAGATGCTTTTGTACAAATTCTTTTAAGAGATGCGGTAAGAGAAGGTGCTAAGATTTTCCCTATTACAACACTTTTAAAAGCGTTTGGTTTAAATAATCGTGAGATCCTTAAAATCTTTAAAAACAATGAATTTATCAAACGTTCATTGGAAGCGGAAATTTACAATGCTAAGGATTTTTTAAGCAATGTTGATCCTGAAATCAAAAACCTTTTAAAAGAATTTAGAGATGGTAAAACTGATTTAAGAAGAAAAGGGATCGCTTCTGATCAGAAATTAAGATCACTTGTTAATGAATATGTAACGCTTGAGAAACAATATAATGCGCTAAAACAAACAAGTCCAAATGATTCTAGTTTAACTGCACTTGAACTGGAAATGGAAAACAAAATGGATAGTGTTATTACTGAAAGAGCTGCTAAACACATTGTCAATGAACTTTCTATTTCACTGCGTGATATTGAAAACACTGAAGAGTGTCATGAAGTGAGTTTCCATGCACTTTTATGTGCGCGTTTTTTTAGAAACAAGAGGTACAACCTCTCTAATGCAGGGAGATACAAAGTATCTAGAAAGTTACGTTTAACAGAACGTATTTATCAAAAAACTCTGGCATGTGATTTGTTCTTAAAAGATGGCAAGCTACTTTTAAAAAAAGGTACTTTACTTTTAAAAGAAGAGATTGACAAAATCAAACAAGCTGCTAAGAACAATGAAATTAGTTTTGTTAATAAAATGCAACTTACAACTGATGGTAAGGCTGTTGATTTAGCAAAAGAATCACTCTTTTATGAAACGATAGATGTATATATCACTAATGATAATCTTAGTGTTTCAGTACCAGTTATAGGGATCCATAACGAAAATGATCTGAACAAAGCAATGACTCTCAGTGATTTCATCGCTTCAATTAGTTATGTGATTAATTTACCTTATGGAATTGGTAAATATGATGATATTGATCACCTTGGTAATAAGCGGGTTAAGTTAATTAATGAATTAATTACTGCTAAATTAGAAAGCGGCTTCACTAGAATGGAGCGCTTTTTAAAAGAAAAGTTAACTATTGCTGATGGAGTTAACCGTGGCCAGCAAATTAATGAAGAGGGTCAGGTTATTGAACAAGGTGAAAAAAAGGAATTAACTATTAAATCTTTAATCAACTCAAAACCAATTCAAATTGTGATTAAAGACTTCTTCAATACCCACCAATTAACCCAATTTTTAGACCACCAAAACCCTTTATCAGAATTGAGTAATAAAAGAAGGATTTCAGCAATGGGACCTGGGGGAATATCAAGAGAGGACCCTAATTTAGATATCCGTGATGTGCATTATTCTCAGTACGGTAGAATTTGCCCTATTGAAACACCTGAAGGGATGAACATAGGGTTGATCATGTCTTTAGCTAGCTTTGCTAAGATTGATGAAAACGGATTTTTAATGGCACCTTATCGCAAAATCAAAGCTGGGGTAATTACTGATGAGGTGGAATATTTAACTGCGCTTAGAGAAGATGAACATATTATTGCTGAGATCTCTTCACTTGTCAATATTAGTAACGATAACAAGATCTTAGATAAGGAAATTATTGGTAGGTATCGATCTATGCAAGGACTTTATGATCCTTTAAAGATTGATTACATTGATGTAGCACCACACCAAGTTGTTTCCATTGGTTCTTCTTTAATCCCCTTTTTGGAAAATGATGATTCAGCTAGAGCATTAATGGGAACCAACATGCAACGTCAGGCCTATCCTTTAATAAAGCCATATGCTCCTGCAGTAGGTACTGGTCAAGAACACAAAATTGCTAGTGATTCAGGTTTAACAATGTCCTCTCCTTGCTCAGGTGTTGTTAGTTATGTTGATAACAGTAAGATTATTATTACAAGTGATAGTTCTAAAAAAGAGACAGTTAACCTAGTTAAATTTGAACGTTCCAACCAAAATACTTGTTATAACCACAAACCAATTGTTGAAATAGGCCAAAGGGTTAATAAGGATGAAATCATTGTTGATGGCCCTGCTGTTAATAAGAGTGAGTTGGCATTAGGACAGAATGTTTTAGTTGCTTTTACAACTTGA", "aa": "MSQKSNFFQKRYSPTATRRYYGKIETNFIQPNLADIQIKSYQKFLDHDLEKLIASYFPIKSPNDRYTINFRGLHRTEPERDEAQSRAQSKTYEVGIYADLELVDNDKGTVKKARKSKKNIASNTNGVFLASMPLITHDGVFIINGIEKFVISQITRSPGIYMLTKSQLKLSNSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGLNNREILKIFKNNEFIKRSLEAEIYNAKDFLSNVDPEIKNLLKEFRDGKTDLRRKGIASDQKLRSLVNEYVTLEKQYNALKQTSPNDSSLTALELEMENKMDSVITERAAKHIVNELSISLRDIENTEECHEVSFHALLCARFFRNKRYNLSNAGRYKVSRKLRLTERIYQKTLACDLFLKDGKLLLKKGTLLLKEEIDKIKQAAKNNEISFVNKMQLTTDGKAVDLAKESLFYETIDVYITNDNLSVSVPVIGIHNENDLNKAMTLSDFIASISYVINLPYGIGKYDDIDHLGNKRVKLINELITAKLESGFTRMERFLKEKLTIADGVNRGQQINEEGQVIEQGEKKELTIKSLINSKPIQIVIKDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKAGVITDEVEYLTALREDEHIIAEISSLVNISNDNKILDKEIIGRYRSMQGLYDPLKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPAVGTGQEHKIASDSGLTMSSPCSGVVSYVDNSKIIITSDSSKKETVNLVKFERSNQNTCYNHKPIVEIGQRVNKDEIIVDGPAVNKSELALGQNVLVAFTT", "aa_hexdigest": "e1edf472b24a7ab2df8bbdfc56c4613a", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoB" ], "truncated": "5-prime", "pseudogene": { "start": 435641, "stop": 438640, "observations": { "mutation": [ 435946 ], "stop": [ 435946 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47583", "query_cov": 1.0, "subject_cov": 0.6460431654676259, "identity": 1.0, "score": 1720.0, "evalue": 0.0, "gene_start": 1, "gene_end": 898, "reference_start": 1, "reference_end": 898, "reference_sequence": "MSQKSNFFQKRYSPTATRRYYGKIETNFIQPNLADIQIKSYQKFLDHDLEKLIASYFPIKSPNDRYTINFRGLHRTEPERDEAQSRAQSKTYEVGIYADLELVDNDKGTVKKARKSKKNIASNTNGVFLASMPLITHDGVFIINGIEKFVISQITRSPGIYMLTKSQLKLSNSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGLNNREILKIFKNNEFIKRSLEAEIYNAKDFLSNVDPEIKNLLKEFRDGKTDLRRKGIASDQKLRSLVNEYVTLEKQYNALKQTSPNDSSLTALELEMENKMDSVITERAAKHIVNELSISLRDIENTEECHEVSFHALLCARFFRNKRYNLSNAGRYKVSRKLRLTERIYQKTLACDLFLKDGKLLLKKGTLLLKEEIDKIKQAAKNNEISFVNKMQLTTDGKAVDLAKESLFYETIDVYITNDNLSVSVPVIGIHNENDLNKAMTLSDFIASISYVINLPYGIGKYDDIDHLGNKRVKLINELITAKLESGFTRMERFLKEKLTIADGVNRGQQINEEGQVIEQGEKKELTIKSLINSKPIQIVIKDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKAGVITDEVEYLTALREDEHIIAEISSLVNISNDNKILDKEIIGRYRSMQGLYDPLKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPAVGTGQEHKIASDSGLTMSSPCSGVVSYVDNSKIIITSDSSKKETVNLVKFERSNQNTCYNHKPIVEIGQRVNKDEIIVDGPAVNKSELALGQNVLVAFTTWNGYNYEDAIVISERLVKEDILTSLTINEYVAQCLSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKVSHGGDGIVSAVKRFSIANGDELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPIEDMPHLEDGTPVDILLNPLGVPSRMNIGQIFETHLGYAAHKLAVRSLISSCFDQNKAKEFAIEINQPQARVERLIKGLKNQINDRNIKSEKEALEKLDNSDISLVLKEIGMSFDDLIYKIATPIFQGVNFLDLQDVMQEAGLDPQKNQGKFKLIDGRSGMPFERPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEVWALEAYGAAYNLQELLTIKSDDVQGRNRAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQSDGEQDEFFNDFEFDTEGY" }, "paralog": false, "identity": 0.998998998998999, "score": 1931.0, "evalue": 0.0, "description": "Internal stop codon at 435946. Nonsense mutation around 435946.", "psc": { "uniref90_id": "UniRef90_P47583", "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47715", "cog_id": "COG0085", "cog_category": "K", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47583", "EC:2.7.7.6", "UniRef:UniRef50_P47715", "COG:COG0085", "COG:K", "KEGG:K03043", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_P47715", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47715" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "KKNAAMEMLJ_782", "locus": "GCA000027325_00778" }, { "type": "cds", "sequence": "contig_1", "start": 438730, "stop": 439236, "strand": "-", "frame": 3, "gene": "ssuE", "product": "putative protein MG342", "db_xrefs": [ "COG:C", "COG:COG0431", "GO:0005524", "GO:0005829", "GO:0010181", "GO:0016491", "RefSeq:WP_009885799.1", "SO:0001217", "UniParc:UPI000013965C", "UniRef:UniRef100_P47584", "UniRef:UniRef50_P75270", "UniRef:UniRef90_P47584" ], "nt": "ATGTCAAAAGATCAAAAGTCATTAATTTTAATGCTCTCCAATTCGCAACATTCCATTAACAGGAAATTTGCTAATGAATTAAAACATTCTTTAAGTTGTGAATTGATTGAGTTAAAAGACTATCAAGTTGATTTTTATTCTGTTGATTTAGAAGCAACTAACTTTCCTGACAAAATTAAAACTTTAGTTAGAAAAATCAAAGAACATAGCAATTTAATTTTTGTTACCCCTGAACACAACGGTTTTATCCCTGCTTTTGCTAAAAACATCATTGACTGGATGACTCGTGATGAGCAGTATGGTAGAAATCAATTTTTAAAAGGTCTTAATGGAGTTATTTGTTGTGTAACTCCAGCAACTGCGGGTGGGGGTAAAACTGTTCTAGAATTACTAGATAAATTTTTAAGTTTTAGCGGTCTTAATGTTAAAGGAACAGTTTTAGTCAACGGTTATCATGAGAACTTTGATTTTAAGCCTTTTATTGAACAAGTTAAAAAATTAATATAA", "aa": "MSKDQKSLILMLSNSQHSINRKFANELKHSLSCELIELKDYQVDFYSVDLEATNFPDKIKTLVRKIKEHSNLIFVTPEHNGFIPAFAKNIIDWMTRDEQYGRNQFLKGLNGVICCVTPATAGGGKTVLELLDKFLSFSGLNVKGTVLVNGYHENFDFKPFIEQVKKLI", "aa_hexdigest": "3f163e57e377939a2d444c66587e0aa1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013965C", "ncbi_nrp_id": "WP_009885799.1", "uniref100_id": "UniRef100_P47584", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013965C", "RefSeq:WP_009885799.1", "UniRef:UniRef100_P47584" ] }, "ips": { "uniref100_id": "UniRef100_P47584", "uniref90_id": "UniRef90_P47584" }, "psc": { "uniref90_id": "UniRef90_P47584", "gene": "SsuE", "product": "Uncharacterized protein MG342", "uniref50_id": "UniRef50_P75270", "cog_id": "COG0431", "cog_category": "C", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0010181", "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75270" ], "product": "Uncharacterized protein MG342 homolog" }, "genes": [ "ssuE" ], "id": "KKNAAMEMLJ_783", "locus": "GCA000027325_00779" }, { "type": "cds", "sequence": "contig_1", "start": 439315, "stop": 439497, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTGATTTGGAATTCCACTTAATTGTTGAAATAATGAAAGGTTTTAATTTTGATGAGAATTGTATCTGTTTACTTGAAGGAAATAAAGAACAAAACCCCAATTTACCAACCGTTAGTTTAGATACTAAGGTTGCTGATATTAAGACTTATCAAGGTTGCTATTTTCTCAATAATTAA", "aa": "MIFDLEFHLIVEIMKGFNFDENCICLLEGNKEQNPNLPTVSLDTKVADIKTYQGCYFLNN", "aa_hexdigest": "c9af8b3911cbb767f1463288a6edf938", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6944.911500000004, "isoelectric_point": 4.395041084289551 }, "id": "KKNAAMEMLJ_784", "locus": "GCA000027325_00780" }, { "type": "cds", "sequence": "contig_1", "start": 439594, "stop": 439989, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAATAGTTTTTTTATTAATTAATTCACCAGAAGAAAAAACAACTATTAAGCTTTCTGATTCGCTAATTAAAGAAATTAAAAAACAAAAAATAAAACATGAAGTTTATTCTTTAAGATCATTTCAAAATAAGTTTTTTAAGTTAGTTAGAAAATTAGAAAAAAAGCACAAGTCTAAAAATAACGTAATGTTTGTTGAACTAAACGGTGTGTTTGGTTATGAAACTAACTTTGAAAAAAGTAACATTGATTTTAGTTTCACTAGTTTTCAAGACAGATTCACAATTGAAAAAAAGTTAAAAATCACCAGCCATTTTATCTTGCCTCATAAATACTTGTTAAAAAATTTAGAAAACATTAAGCATCCTAACTTTGAGCAAAAGAACTTAATTTGA", "aa": "MKIVFLLINSPEEKTTIKLSDSLIKEIKKQKIKHEVYSLRSFQNKFFKLVRKLEKKHKSKNNVMFVELNGVFGYETNFEKSNIDFSFTSFQDRFTIEKKLKITSHFILPHKYLLKNLENIKHPNFEQKNLI", "aa_hexdigest": "6cf12a82c5c16982bc8a65fc2ac0d402", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15738.292599999992, "isoelectric_point": 9.8639368057251 }, "id": "KKNAAMEMLJ_785", "locus": "GCA000027325_00781" }, { "type": "cds", "sequence": "contig_1", "start": 440116, "stop": 440355, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCATAAAATTAATGATCAAACCCTCGTTAATGAGCAACGCCTATTAGCTTATGATTTTTTTCAAAATAGCAATAAGGTTGGACTTTTATCAACATTACAATATCTAGATTTTGTTAATTTTTTAGTTAGATCTAAAAAAGTAAATTATCTGCTTGTTAACAAAGTAAGTCTTCCAATATATCAAAAGATTTATTTTGATAACTTCCCTTTTTTAGGTTTTGATAATAACTTGTGA", "aa": "MSHKINDQTLVNEQRLLAYDFFQNSNKVGLLSTLQYLDFVNFLVRSKKVNYLLVNKVSLPIYQKIYFDNFPFLGFDNNL", "aa_hexdigest": "cfd8b67c0df74949332d557a25b5fc2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9368.678999999995, "isoelectric_point": 9.00418643951416 }, "id": "KKNAAMEMLJ_786", "locus": "GCA000027325_00782" }, { "type": "cds", "sequence": "contig_1", "start": 440359, "stop": 440643, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAAAGCTTTCTAATAATATTTTGAAAGAACTACCAGTTTTAAATAAGACTTATAAAAATTTAAAGTTACCAGTGTTTTTGGTTTTAGCTCAAAATGATTTATTTATGCCAACAAAATTAACATTAAGCTATTTCAATAAATACCTAATTAAGAACAATAATTTGCAATCATCTGTTATCTTAAACAGTGAACACCAAATGTTTAATAGTAAATATGAAAGCTTTTGTAAAGCAATGGATGATATCCTGAATCATAATAAACTAAGTAAAATTTATTAA", "aa": "MAKKLSNNILKELPVLNKTYKNLKLPVFLVLAQNDLFMPTKLTLSYFNKYLIKNNNLQSSVILNSEHQMFNSKYESFCKAMDDILNHNKLSKIY", "aa_hexdigest": "9eedfe8da8d8eb0270fad1306074968c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11015.864299999994, "isoelectric_point": 9.608835411071777 }, "id": "KKNAAMEMLJ_787", "locus": "GCA000027325_00783" }, { "type": "cds", "sequence": "contig_1", "start": 440671, "stop": 440880, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGCTGCTGTTTGTAGTTATGCCATGAATTTATTAAATGCTAAACGTGTTGAAAAACTCATACTTTTAGCACCTCTTTCATACTGTAACTTATTGAGATATTTCAAAATTAAAAGTTCTTTTAAAAAAGATAAGGCAGAAAGAATGGCTAATTTTAAAGCAATGTTTCAAACTAAATTTAGTAATTTAACTGATGAAAACAGCTGA", "aa": "MGAAVCSYAMNLLNAKRVEKLILLAPLSYCNLLRYFKIKSSFKKDKAERMANFKAMFQTKFSNLTDENS", "aa_hexdigest": "53fff15810565e49c23b518d71d95a70", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7917.299999999997, "isoelectric_point": 9.745379447937012 }, "id": "KKNAAMEMLJ_788", "locus": "GCA000027325_00784" }, { "type": "cds", "sequence": "contig_1", "start": 441001, "stop": 441180, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAGGTTTTCAGATCTTAATCAGATTGATATTTCTAAGCTTGAAGTTTTCTTTCAGCCAGCAAAAAAAACAAAAAAACAAACGGTTGTTTTTGCACATGGATTTTCTGTTTTTCATAGCTACTTTCAAAGTTTTTATAAAACCCTTACTGATTATGACTATTACGCTCCACTTTGA", "aa": "MKRFSDLNQIDISKLEVFFQPAKKTKKQTVVFAHGFSVFHSYFQSFYKTLTDYDYYAPL", "aa_hexdigest": "aab2fd7edb693215896599d9c390f889", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7066.0082, "isoelectric_point": 9.30203037261963 }, "id": "KKNAAMEMLJ_789", "locus": "GCA000027325_00785" }, { "type": "cds", "sequence": "contig_1", "start": 441347, "stop": 441562, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACCTCTTTACAAAACCGACTGTTTTTAAAGTTGCTAACTCTAAGAATTTAGAAAATATCTATAAAACTTTTACTAGTATTAAAAATGCTGCTTTCAAAGAAATTGAAAAGCTAAGAAAAGAAGGGTTGATTTCTAAAAATAATCAAATTGAATTAACCGTTGGAATTAATAAAAAAATACCCAAAAAATTAAAGGATAATCTCGCACTTTGA", "aa": "MNLFTKPTVFKVANSKNLENIYKTFTSIKNAAFKEIEKLRKEGLISKNNQIELTVGINKKIPKKLKDNLAL", "aa_hexdigest": "68d5682c9058b674823a24a2206c3b2e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8121.540599999993, "isoelectric_point": 10.056761741638184 }, "id": "KKNAAMEMLJ_790", "locus": "GCA000027325_00786" }, { "type": "cds", "sequence": "contig_1", "start": 441755, "stop": 441892, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGATTATAAGTTTTCACTAGAAGACAAAATAGTTATCCATAAAACTAATTCACTAGTAGAACAAATTGAGAAATTTTTAGAGAAATATAATTTTTTAGGTTGCCTAAAAGTGATTAATAAGTTTGTTTTATGA", "aa": "MDDYKFSLEDKIVIHKTNSLVEQIEKFLEKYNFLGCLKVINKFVL", "aa_hexdigest": "6c768a02f337ced0c35d4b18980563ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5394.306199999999, "isoelectric_point": 6.534574317932129 }, "id": "KKNAAMEMLJ_791", "locus": "GCA000027325_00787" }, { "type": "cds", "sequence": "contig_1", "start": 442031, "stop": 442276, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGAAATAAATAAATATGGTTCAATAGCTGATCTTTATATTGAAGGTTCTGATCAATATCGGGGTTGGTTCAACTCTTCTTCAAATTGCGGAATTATTCAAAATGATTTAATCCCTTTTAAATCACTTGTTTCACATGGTTTTACACTTGATGAAAATGGCAATAAAATGTCAAAGTCATTAGGAAACATAGTTGATCCTTTAAAAATTTGTGATCAATATGGAGCGGATATATTAAGGTTGTGA", "aa": "MEINKYGSIADLYIEGSDQYRGWFNSSSNCGIIQNDLIPFKSLVSHGFTLDENGNKMSKSLGNIVDPLKICDQYGADILRL", "aa_hexdigest": "4bb8445ed36ea9a405cc1e4836f8a11b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 72, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.1401150067850364e-26, "score": 88.53526306152344, "start": 10, "stop": 81 } ], "seq_stats": { "molecular_weight": 9033.086399999993, "isoelectric_point": 4.791606712341309 }, "id": "KKNAAMEMLJ_792", "locus": "GCA000027325_00788" }, { "type": "cds", "sequence": "contig_1", "start": 442682, "stop": 442954, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATACATGTTGTTGATAATGAGGGAACAGGTATTGTTCACAGCTCCCCTGCATTTGGAATTGATGATTTTTATCTTTGTCAAAAAAACAAGATTAAAGAAGTTTTGATTTCTATTGATGAGAAAGGTGTATTTAATAACTTACTTAATGATAAAGAACTTGAGAATTGTTTTTATCTTAAAGCAAATGATCTAATTATTAATCGTTTAAAACAAAACAATAGCTTTATTTTTTCTGAAGTTATTTCCCACCGCGAACCACATGATTGA", "aa": "MGIHVVDNEGTGIVHSSPAFGIDDFYLCQKNKIKEVLISIDEKGVFNNLLNDKELENCFYLKANDLIINRLKQNNSFIFSEVISHREPHD", "aa_hexdigest": "6224d419f170cbc0dc5f76aa7e67cbae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10306.548399999989, "isoelectric_point": 5.225913429260252 }, "id": "KKNAAMEMLJ_793", "locus": "GCA000027325_00789" }, { "type": "cds", "sequence": "contig_1", "start": 443321, "stop": 443686, "strand": "-", "frame": 2, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029621904", "UniRef:UniRef90_UPI0029621904" ], "nt": "GTGGGACATGCTCTTAACAAGATTTTAAAAGACTTCATTTTACGTAGTTGGTTATATGAAGGATATGATGTTGTTTTTATTCCTGGTTGGGATTGTCATGGACTACCAATAGAACATGCAGTTAGTAAGAAAAACCCTAGTAGTTATAGCAATCTTTCAACTGTTGAAAAAAGAAAATTATGTCATCAGTTTGCACTTTCACAAATTGCAGTTCAAAAAGAACAATTTCAAAGACTGGGACTTTTAAATGATTTTCAAAACTGTTATTACACAATAGATGAGAGTTTTCAATTTAAGGAACTTGAACTATTTTTACAAGCAATTAAAAAAGGGCTCATTTTTCAAGATTTAAAACCAACTTATTGA", "aa": "MGHALNKILKDFILRSWLYEGYDVVFIPGWDCHGLPIEHAVSKKNPSSYSNLSTVEKRKLCHQFALSQIAVQKEQFQRLGLLNDFQNCYYTIDESFQFKELELFLQAIKKGLIFQDLKPTY", "aa_hexdigest": "7e4deceb4c8eb1dcf961f4576070d4c5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 443280, "stop": 443734, "observations": { "deletion": [ 443359 ], "stop": [ 443293 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029621904", "query_cov": 0.9752066115702479, "subject_cov": 0.7712418300653595, "identity": 0.941, "score": 230.0, "evalue": 6.68e-76, "gene_start": 1, "gene_end": 118, "reference_start": 17, "reference_end": 134, "reference_sequence": "MKILHDGPPYANGSIHVGHALNKILKDFILRSWLYEGYDVVFIPGWDCHGLPIEHAVSKKNPSSYSNLSTVEKRKLCHQFALSQIAVQKEQFQRLGLLNDFQNCYYTIDESFQFKELELFLQAIKKRAHFSRFKTNLLITNFKNFTCWSGNWI" }, "paralog": false, "identity": 0.9867549668874173, "score": 306.0, "evalue": 2.58e-108, "description": "Internal stop codon at 443293. Frameshift due to deletion around 443359.", "psc": { "uniref90_id": "UniRef90_UPI0029621904", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0029621904", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029621904", "UniRef:UniRef50_UPI0029621904" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029621904", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029621904" ], "product": "class I tRNA ligase family protein" } }, "id": "KKNAAMEMLJ_794", "locus": "GCA000027325_00790" }, { "type": "cds", "sequence": "contig_1", "start": 443922, "stop": 444089, "strand": "+", "frame": 3, "gene": "spoU", "product": "tRNA/rRNA methyltransferase SpoU type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A917CEE2" ], "nt": "ATGTATAAATCAGTAATAAACATAGTTTTATTTTGTCCAGAAATTCCTAATAACACTGGCAACATCGTACGTAGTTGCACTGCTTTTAAAGCTAATCTACACTTAATTAAACCTTATGGCTTTTTCTTAAATGATAAAAGGATGGTTAGAGCTGGTTTAAATTGTTGA", "aa": "MYKSVINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNC", "aa_hexdigest": "e695ddec9a2106217f086fb914ef11b9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0023B9359B", "query_cov": 0.9090909090909091, "subject_cov": 0.819672131147541, "identity": 0.62, "score": 65.9, "evalue": 3.47e-13, "valid": false, "product": "tRNA (cytidine(34)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_A0A917CEE2", "kegg_orthology_id": "K03216" }, "pscc": { "uniref50_id": "UniRef50_A0A917CEE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A917CEE2" ], "product": "tRNA/rRNA methyltransferase SpoU type domain-containing protein" }, "genes": [ "spoU" ], "id": "KKNAAMEMLJ_795", "locus": "GCA000027325_00791" }, { "type": "cds", "sequence": "contig_1", "start": 444207, "stop": 444320, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAATAAATTACCAAACGAACTTTACTTTGTTTTTGGTCAGGAAACAAAGGGATTACCTAAAACAATCATGGATAACTTTAAACAAAACCAAATTAGAATTCCCATTTGA", "aa": "MTNKLPNELYFVFGQETKGLPKTIMDNFKQNQIRIPI", "aa_hexdigest": "b71224ab4b6b21218cc93ad966e91af1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4368.0843, "isoelectric_point": 9.398732948303223 }, "id": "KKNAAMEMLJ_796", "locus": "GCA000027325_00792" }, { "type": "cds", "sequence": "contig_1", "start": 444413, "stop": 444754, "strand": "+", "frame": 2, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0030488", "GO:0036265", "GO:0043527", "SO:0001217", "UniRef:UniRef50_P47589", "UniRef:UniRef90_P47589" ], "nt": "GTGCGCTTAAGAAAAGTTAAAAACGCTCTTTTAAAAATTAATCAAAGTCCTTATTTTTATTCAAAAGATAAGTTTGCTAAGTTTACTAAAAAACAATTAGTGCTGGAATTGGGTTGTGGTAAGGGTACTTTTTTAATCAAAGAAGCACAAAAAAATAACAATTTTCTTTTTATAGGAATTGAACGTGAACCTACAATTGTTTTAAAAGCAATTAACAAAATTAACAAGTTGGATTTTAATTTGGAAAATATCTTATTGTTGTGTACAGATGCAAAACAACTTGATGATTATTTTCAAGCTGAATCTGTTCAAAAAATCTTTATTAATTTCCCTGATCCTTGA", "aa": "MRLRKVKNALLKINQSPYFYSKDKFAKFTKKQLVLELGCGKGTFLIKEAQKNNNFLFIGIEREPTIVLKAINKINKLDFNLENILLLCTDAKQLDDYFQAESVQKIFINFPDP", "aa_hexdigest": "ebf3cc8848cdb8fad7990322f4f92b93", "start_type": "GTG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 444413, "stop": 445045, "observations": { "mutation": [ 444752, 444812 ], "stop": [ 444752, 444812 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47589", "query_cov": 1.0, "subject_cov": 0.5380952380952381, "identity": 1.0, "score": 224.0, "evalue": 4.29e-73, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MRLRKVKNALLKINQSPYFYSKDKFAKFTKKQLVLELGCGKGTFLIKEAQKNNNFLFIGIEREPTIVLKAINKINKLDFNLENILLLCTDAKQLDDYFQAESVQKIFINFPDPWPKKRHIQRRLTSPDFLKLFWNLLVKNGLIEFKTDNDKLFEYTLTTLQENSQIFEIIHQITDLNNSEFSFQNSITEYEQRFMELEIPIKKLVIKKII" }, "paralog": false, "identity": 0.9857142857142858, "score": 407.0, "evalue": 9.91e-148, "description": "Internal stop codon at 444752, 444812. Nonsense mutation around 444752, 444812.", "psc": { "uniref90_id": "UniRef90_P47589", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_P47589", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0030488", "GO:0036265", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47589", "EC:2.1.1.33", "UniRef:UniRef50_P47589", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0030488", "GO:0036265", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_P47589", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47589" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "KKNAAMEMLJ_797", "locus": "GCA000027325_00793" }, { "type": "cds", "sequence": "contig_1", "start": 444890, "stop": 445045, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAAAATAGTCAAATTTTTGAAATTATCCATCAAATAACTGATCTTAACAATTCTGAATTCAGTTTTCAAAATAGTATCACTGAATATGAACAGCGCTTTATGGAATTAGAAATTCCAATTAAAAAACTAGTGATTAAGAAAATAATTTAA", "aa": "MQENSQIFEIIHQITDLNNSEFSFQNSITEYEQRFMELEIPIKKLVIKKII", "aa_hexdigest": "7a662a05397a943d6bd9c36add1aa56d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6172.043699999997, "isoelectric_point": 4.876922035217285 }, "id": "KKNAAMEMLJ_798", "locus": "GCA000027325_00794" }, { "type": "tRNA", "sequence": "contig_1", "start": 445078, "stop": 445153, "strand": "+", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 71.9, "nt": "GCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 445084, 445086 ], "id": "KKNAAMEMLJ_15", "locus": "GCA000027325_00795" }, { "type": "cds", "sequence": "contig_1", "start": 445197, "stop": 445718, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MG348", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47590", "UniRef:UniRef90_P47590" ], "nt": "GTGGATAGAAAAAACGAACTTGCAAGTCAAGAAGATAAAGACAATTCCAATAAGTTAAGAAGATCAGATGCTTCTGCAGTTCGCTATAGAGAAATAGTTGATTTTATTCAACGTACTTATGGTGGTAATGTAGCTAACTTAATCAATCAATCAAATGTGCATGCTCAAACAGTTGGTAATGATGTAACTAAAGCAATTGTGATTGCTTTTAGAAAAGATAACCTTAATAAGATAAGAGCTAATTTTTATGGTTTGGATAACTCTACAAATCCTAATGCACCTGGATCAGGTCAGGGTGATTCAACCCCACCAGCTTCAAGTGGTGAAGGGGGCGGTAGTGATGGCAGTTCAGGTGGTGACTCAAGTAGTGGTAATGGACAAAATACTACTCCTACTTCACCACAATCTTCTCAACCAGCAGTTCAAAGATCACAAAAATCTTATGGAATTAAACAACACGCAGTAAGAGTTAGTGTTGATTTTTTAAATTTCTTGGATAGTGTTTATACTCCACTAAACTAG", "aa": "MDRKNELASQEDKDNSNKLRRSDASAVRYREIVDFIQRTYGGNVANLINQSNVHAQTVGNDVTKAIVIAFRKDNLNKIRANFYGLDNSTNPNAPGSGQGDSTPPASSGEGGGSDGSSGGDSSSGNGQNTTPTSPQSSQPAVQRSQKSYGIKQHAVRVSVDFLNFLDSVYTPLN", "aa_hexdigest": "9738ae84d53004541df9d7f7c059d0ac", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 445197, "stop": 446018, "observations": { "mutation": [ 445745, 445856 ], "stop": [ 445745, 445856 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47590", "query_cov": 1.0, "subject_cov": 0.5372670807453416, "identity": 0.9940000000000001, "score": 295.0, "evalue": 4.14e-98, "gene_start": 1, "gene_end": 173, "reference_start": 150, "reference_end": 322, "reference_sequence": "MKRLFWNLKHKKAWLVLLLGTGMILSSCSNIDQSFFRELSVESVEKNTAYNQLPVNSTTFRNLVFGTRSYNDGNYVVVIATETDSSQINFLNGSTNQGTSSLSWGGTLGTTIRQVQNRYSTYPNGVKFLIWNDIAPGSVKWNPYARFPVVDRKNELASQEDKDNSNKLRRSDASAVRYREIVDFIQRTYGGNVANLINQSNVHAQTVGNDVTKAIVIAFRKDNLNKIRANFYGLDNSTNPNAPGSGQGDSTPPASSGEGGGSDGSSGGDSSSGNGQNTTPTSPQSSQPAVQRSQKSYGIKQHAVRVSVDFLNFLDSVYTPLN" }, "paralog": false, "identity": 0.9926739926739927, "score": 510.0, "evalue": 1.86e-185, "description": "Internal stop codon at 445745, 445856. Nonsense mutation around 445745, 445856.", "psc": { "uniref90_id": "UniRef90_P47590", "product": "Uncharacterized lipoprotein MG348", "uniref50_id": "UniRef50_P47590", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47590", "UniRef:UniRef50_P47590", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47590", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47590" ], "product": "Uncharacterized lipoprotein MG348" } }, "id": "KKNAAMEMLJ_799", "locus": "GCA000027325_00796" }, { "type": "cds", "sequence": "contig_1", "start": 445854, "stop": 446099, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAAGTTCTTGTAGTAACATCGATCAGTCTTTTTTTCGTGAACTTTCTGTTGAAAGTGTGGAAAAAAATACAGCTTACAACCAATTGCCTGTTAACAGTACAACATTTAGAAACTTAGTTTTTGGAACCAGATCATATAACGATGGTAATTATGTGGTTGTTATTGCAACCGAAACAGATAGTTCCCAAATTAATTTTCTAAACGGTTCTACTAATCAAGGTACAAGTTCTCTTAGTTGA", "aa": "MILSSCSNIDQSFFRELSVESVEKNTAYNQLPVNSTTFRNLVFGTRSYNDGNYVVVIATETDSSQINFLNGSTNQGTSSLS", "aa_hexdigest": "c86275855398790d5a21928bf08b0e14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8910.615799999998, "isoelectric_point": 4.440682792663575 }, "id": "KKNAAMEMLJ_800", "locus": "GCA000027325_00797" }, { "type": "tRNA", "sequence": "contig_1", "start": 446178, "stop": 446265, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(gag)", "amino_acid": "Leu", "anti_codon": "gag", "score": 63.4, "nt": "GTCGGAGTGGTGGAATGGTAGACACGCAAGCTTGAGGTGCTTGTGGTCGTTAAAGACTGTGCCAGTTCAAGTCTGGTCTCCGACACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 446259, 446261 ], "id": "KKNAAMEMLJ_18", "locus": "GCA000027325_00798" }, { "type": "cds", "sequence": "contig_1", "start": 446618, "stop": 446734, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACCCAACTATTACCGTGTGATCAAAAAAGCAACATCATTTTCTGGATTAGAAATGATCTCTCATGCTTATGGTCAAATTGCGGGAGTGGAAGTTGTTGGGTTTTATTTATGA", "aa": "MQPNYYRVIKKATSFSGLEMISHAYGQIAGVEVVGFYL", "aa_hexdigest": "0915135e70d0a1e36d478ffd60a4f290", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4268.9082, "isoelectric_point": 8.119873619079591 }, "id": "KKNAAMEMLJ_801", "locus": "GCA000027325_00799" }, { "type": "cds", "sequence": "contig_1", "start": 446888, "stop": 447130, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAACTTTTTTTTCACCAAAACGCTCTGAAATAACTTTTTGTATTATTGATCCTTTAGATTGGAAAGAATTTAAGCAAAACAAACAATTAAAAGAAAAACTAGTTGAGGCAATGGGAAAAGTTGAATATGACCGAAACTGCTTAGCTTTTGATCAAATCGACAATCTTCAATTTGATAATGCGCTTGAAATCTCTGCTAACTTTGAAGTTAATTTCACTGCAAACCAAAGCGATGTTTGA", "aa": "MRTFFSPKRSEITFCIIDPLDWKEFKQNKQLKEKLVEAMGKVEYDRNCLAFDQIDNLQFDNALEISANFEVNFTANQSDV", "aa_hexdigest": "ce70d05047fff2972ce81ab9881046c1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9427.553499999996, "isoelectric_point": 4.678099250793457 }, "id": "KKNAAMEMLJ_802", "locus": "GCA000027325_00800" }, { "type": "cds", "sequence": "contig_1", "start": 447248, "stop": 447589, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACAAATTACTGATTGCATCATCAACAGTAGTACTCAAGAGAATGAGCTTGATTTTCAAAAGTTAGAAATGATGTTTTTTCAAATAGTAAAAAATGATACAGCTCCCATTTTAGAAACAGTTTCAAACAACAAAGACTTTTTTTATAAGAATGAAATTTTAGATGAATCAACAAAAAAAGCAATAACAGACTGTCATGTAAACTTCAATTCTGAAAAATACCTTTTTCTTCTATATGGAAAGATAGATGAATCGCAATTGCAATTAGTTAGACAATTGAGAAGTGATTATCAACTATTAGATAAAGTAATTAATTTAGTATTAGACTTTTCCTTTTGA", "aa": "MQQITDCIINSSTQENELDFQKLEMMFFQIVKNDTAPILETVSNNKDFFYKNEILDESTKKAITDCHVNFNSEKYLFLLYGKIDESQLQLVRQLRSDYQLLDKVINLVLDFSF", "aa_hexdigest": "4b32108ca77a32f3d05836aefe90cf79", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13359.054599999985, "isoelectric_point": 4.549472618103027 }, "id": "KKNAAMEMLJ_803", "locus": "GCA000027325_00801" }, { "type": "cds", "sequence": "contig_1", "start": 447790, "stop": 448005, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTTCACCAGATCAAAAAGATAAAAAGTCATTATTTAAAAAACATTTTGCTTATTTGAAATTAACTGAAAAAGAACTTAATGATCCTAAGATACAAACACTTTTAGAAGTTGCGTGGAATCATTTTGATCAGTGTAGAAAAATTAAAGAAAGAAGATGTAGTAATAAAGGTGATATCCATCTTCAAGCTGTTAGAGAGTGGTTACCTTGA", "aa": "MKISPDQKDKKSLFKKHFAYLKLTEKELNDPKIQTLLEVAWNHFDQCRKIKERRCSNKGDIHLQAVREWLP", "aa_hexdigest": "55370079a5ce544a8a31a663ee684bed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8563.8949, "isoelectric_point": 9.553714942932128 }, "id": "KKNAAMEMLJ_804", "locus": "GCA000027325_00802" }, { "type": "cds", "sequence": "contig_1", "start": 448189, "stop": 448449, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTGATTCCAATGATCCAAAATTTACCAGTAACTCCAAGTGATAAAAATAGTTTTGGTTATGCATACAAAAAGATATCTAATCTTTTTGAAACTGAAAAGAAAACAAAGAAAAGGATATTTTTTTTCAATTTACAAAAGAATAACATTAACAATATGGCAGCTTGTATGTTAACTTGTGAAATAGCTAAGAAAAATATAAAAGTTGCTTTAATTTATTGTGAAGAATTTGTTAGCAGATATGACAAAAGTTATTGA", "aa": "MDLIPMIQNLPVTPSDKNSFGYAYKKISNLFETEKKTKKRIFFFNLQKNNINNMAACMLTCEIAKKNIKVALIYCEEFVSRYDKSY", "aa_hexdigest": "f509a4fcb46e1b13e096fd9f505fc189", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10100.777699999995, "isoelectric_point": 9.324723243713382 }, "id": "KKNAAMEMLJ_805", "locus": "GCA000027325_00803" }, { "type": "tRNA", "sequence": "contig_1", "start": 448783, "stop": 448864, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 59.5, "nt": "CCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 448816, 448818 ], "id": "KKNAAMEMLJ_16", "locus": "GCA000027325_00804" }, { "type": "cds", "sequence": "contig_1", "start": 449408, "stop": 449617, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTAATAAACTGGTTTTTTTGCCAAAAAAATCATACCATCAGTTATTTAATATTACTTTTAGTGCAATGATGCTTGCTTTGGCCATCATTACATCATTAATTAGTGAATTTATTAGTATTCCTTTTTTCTCACAACTAAAACTAACTTTTGATATTTCCGTTGTTTTTTTAGTTGCATGTGCTTTTTTTGTGTCACTAGGTTGA", "aa": "MNGNKLVFLPKKSYHQLFNITFSAMMLALAIITSLISEFISIPFFSQLKLTFDISVVFLVACAFFVSLG", "aa_hexdigest": "c07cd3cd2697cda8ebd3008fec0aad61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7725.240299999996, "isoelectric_point": 9.035582542419434 }, "id": "KKNAAMEMLJ_806", "locus": "GCA000027325_00805" }, { "type": "cds", "sequence": "contig_1", "start": 449665, "stop": 449826, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGGACAAATTTTTAATTGATGTTATTGTAGAAATCCCTAAAAACAGCAAAATAAAGTATGAGTATGATCGTCAAACTGGTCAAATTCGCGTTGATAGAATCCTATTTGGAAGTGAATCATATCCACAAAACTACGGTTTTATTAAAAATACATTAGATTGA", "aa": "MDKFLIDVIVEIPKNSKIKYEYDRQTGQIRVDRILFGSESYPQNYGFIKNTLD", "aa_hexdigest": "1e81362bdd10d07a3c9581d7b9a7943b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 45, "aa_cov": 0.8490566037735849, "hmm_cov": 1.0, "evalue": 8.833373649973968e-18, "score": 59.66098403930664, "start": 8, "stop": 52 } ], "seq_stats": { "molecular_weight": 6300.1115, "isoelectric_point": 6.145397758483886 }, "id": "KKNAAMEMLJ_807", "locus": "GCA000027325_00806" }, { "type": "cds", "sequence": "contig_1", "start": 449887, "stop": 450105, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "GTGCCTACAAGAATTGTAGGAGCACTTGAGATGATTGATGATGGGGAAATTGATACTAAGTTATTAGGAGTTATTGATTGTGACCCTAGATATAAAGAAATTAATCAAATTAGTGATTTACCTAAACATAGAATAGAAGAAATTCTTATCTTTTTAAAAACTTATAAATTACTTCAAAAAAAGACTGTAATTATTAAGGGTTTAAAAGATGTTTGTTGA", "aa": "MPTRIVGALEMIDDGEIDTKLLGVIDCDPRYKEINQISDLPKHRIEEILIFLKTYKLLQKKTVIIKGLKDVC", "aa_hexdigest": "acb30d4e67b259dd1258e2fa1f954240", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 69, "aa_cov": 0.9583333333333334, "hmm_cov": 1.0, "evalue": 3.8365040099222085e-19, "score": 64.08428192138672, "start": 2, "stop": 70 } ], "seq_stats": { "molecular_weight": 8282.800799999994, "isoelectric_point": 6.551682853698731 }, "id": "KKNAAMEMLJ_808", "locus": "GCA000027325_00807" }, { "type": "cds", "sequence": "contig_1", "start": 450414, "stop": 450722, "strand": "+", "frame": 3, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "COG:COG3331", "COG:R", "EC:3.1.21.10", "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_Q49422", "UniRef:UniRef90_Q49422" ], "nt": "ATGTATTTTGATTTTGAAGCAAAACAAACAAATAAAAGTAATTTTCCAATTGCTCAAATAGCAGAACACCAATTAAATCATTTGAAAAGAATTGACCAAATTGGAGGAGTTAGCTTCTTGCTAATTTACTTTCAAACTAAAGATCAAATTTTTGCATTTCACACTAAGGATCTTCTAGAAACAATAAAAAATCAAGAAAGCAAAACAATTAAAAGGGAATTGATAGAACAAAAATCTCAGAAAGTTCCACTATTGTATCCTGGTATTATTGATTTAATTTCAATAATTCAAAGCTTTAAAAATTATTAG", "aa": "MYFDFEAKQTNKSNFPIAQIAEHQLNHLKRIDQIGGVSFLLIYFQTKDQIFAFHTKDLLETIKNQESKTIKRELIEQKSQKVPLLYPGIIDLISIIQSFKNY", "aa_hexdigest": "765350889a981d7f6d3b937009619494", "start_type": "ATG", "rbs_motif": null, "genes": [ "recU" ], "truncated": "5-prime", "pseudogene": { "start": 450222, "stop": 450722, "observations": { "mutation": [ 450303 ], "stop": [ 450303 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49422", "query_cov": 1.0, "subject_cov": 0.6144578313253012, "identity": 1.0, "score": 202.0, "evalue": 3.09e-65, "gene_start": 1, "gene_end": 102, "reference_start": 65, "reference_end": 166, "reference_sequence": "MINANRGMLLETIVNQTIARLKDHPDIWLEKRFLPIKPIAFRHAHVSGNVSQKSKTDYYGIYKGMYFDFEAKQTNKSNFPIAQIAEHQLNHLKRIDQIGGVSFLLIYFQTKDQIFAFHTKDLLETIKNQESKTIKRELIEQKSQKVPLLYPGIIDLISIIQSFKNY" }, "paralog": false, "identity": 0.9939759036144579, "score": 328.0, "evalue": 3.08e-117, "description": "Internal stop codon at 450303. Nonsense mutation around 450303.", "psc": { "uniref90_id": "UniRef90_Q49422", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_Q49422", "cog_id": "COG3331", "cog_category": "R", "kegg_orthology_id": "K03700", "go_ids": [ "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49422", "EC:3.1.21.10", "UniRef:UniRef50_Q49422", "COG:COG3331", "COG:R", "KEGG:K03700", "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q49422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49422" ], "product": "Holliday junction resolvase RecU" } }, "id": "KKNAAMEMLJ_809", "locus": "GCA000027325_00808" }, { "type": "cds", "sequence": "contig_1", "start": 450734, "stop": 451048, "strand": "-", "frame": 2, "gene": "hupA", "product": "putative MG353-like protein", "db_xrefs": [ "COG:B", "COG:COG0776", "GO:0003677", "GO:0030527", "SO:0001217", "UniRef:UniRef50_P47595", "UniRef:UniRef90_P47595" ], "nt": "ATGGAAAAAACATCAAATACAAGTAAGCCACTTTCTCGTAGTGAAATCAATAAAATAATTGCAGTTGCTACTGGTATAAAAGAAAAAAAAATTAAGGAAATCTTTAAATACCTTAACACATTGTTACTAAATGAATTGGTAAGTAGAAGTGTTTGTATATTACCTGAAAATTTAGGTAAATTAAGGATTACTATTAGAAATGCACGTTATCAGAAGGATATGCAAACAGGTGAGATTAGACATATCCCACCAAAACCATTAGTGCGCTACAGTCCAAGCAAAACGATCAAAGAAACTGCAGCTAAAGTGCGTTGA", "aa": "MEKTSNTSKPLSRSEINKIIAVATGIKEKKIKEIFKYLNTLLLNELVSRSVCILPENLGKLRITIRNARYQKDMQTGEIRHIPPKPLVRYSPSKTIKETAAKVR", "aa_hexdigest": "2f0e07af14c4a3b6afd04bd9a12ba70a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47595", "query_cov": 1.0, "subject_cov": 0.9541284403669725, "identity": 1.0, "score": 193.0, "evalue": 2.41e-62, "valid": true, "gene": "HupA", "product": "Uncharacterized protein MG353 homolog", "uniref50_id": "UniRef50_P47595", "cog_id": "COG0776", "cog_category": "B", "go_ids": [ "GO:0003677", "GO:0030527" ] }, "pscc": { "uniref50_id": "UniRef50_P47595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47595" ], "product": "Uncharacterized protein MG353" }, "genes": [ "hupA" ], "id": "KKNAAMEMLJ_810", "locus": "GCA000027325_00809" }, { "type": "cds", "sequence": "contig_1", "start": 451197, "stop": 451610, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG354", "db_xrefs": [ "RefSeq:WP_009885812.1", "SO:0001217", "UniParc:UPI000013968F", "UniRef:UniRef100_P47596", "UniRef:UniRef50_P47596", "UniRef:UniRef90_P47596" ], "nt": "ATGGAACAAAACAATATTAAAGAACAACTTATTTCTTTCTTTAATCAAGCATGTTCTACCCACCAAGAAAGACTTGATTTTATCTGTTCTACAAGAGAAAGTGATACTTTTTCTAGTGTTGATGTACCACTTGAACCCATTAAAAATATTATTGAAATAACTAAAGATGAAAATCAACAAATTGAAATTACAAAAATAGCTGTTAATAACATTAAAACATTATCTTCTGTTGGTGCAACCGGTCAGTATATGGCATCTTTCTTTTCAACGAATAGTGAGCCAGCTATAATATTTTGCGTCATTTATTTTTTATATCACTTTGGTTTTTTAAAAGATAACAATAAAAAACAAATAATAAAAAAGGCTTATGAAACTATTGCTGATAACATTGCTGACTATTTAAATGAAAATTAG", "aa": "MEQNNIKEQLISFFNQACSTHQERLDFICSTRESDTFSSVDVPLEPIKNIIEITKDENQQIEITKIAVNNIKTLSSVGATGQYMASFFSTNSEPAIIFCVIYFLYHFGFLKDNNKKQIIKKAYETIADNIADYLNEN", "aa_hexdigest": "7aae0a269f2241d48b8b839d81d6664a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013968F", "ncbi_nrp_id": "WP_009885812.1", "uniref100_id": "UniRef100_P47596", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013968F", "RefSeq:WP_009885812.1", "UniRef:UniRef100_P47596" ] }, "ips": { "uniref100_id": "UniRef100_P47596", "uniref90_id": "UniRef90_P47596" }, "psc": { "uniref90_id": "UniRef90_P47596", "product": "Uncharacterized protein MG354", "uniref50_id": "UniRef50_P47596" }, "pscc": { "uniref50_id": "UniRef50_P47596", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47596" ], "product": "Uncharacterized protein MG354" }, "genes": [], "id": "KKNAAMEMLJ_811", "locus": "GCA000027325_00810" }, { "type": "cds", "sequence": "contig_1", "start": 451613, "stop": 452497, "strand": "-", "frame": 2, "gene": "clpB", "product": "Chaperone protein ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "GO:0005524", "GO:0005737", "GO:0016887", "GO:0034605", "SO:0001217", "UniRef:UniRef50_P47597", "UniRef:UniRef90_P47597" ], "nt": "TTGCACTTAGGTGATGAAATCAAAAAAAGAGTTAAAGGACAAGATGAAGCCATCGATGCTGTTGTTAACACTGTAATTAGAGGTAGAGTAAATATAAATGACCCAAACAAACCTATTGGTTCTTTCATCTTTTTAGGTTCTACTGGTGTTGGTAAAACTGAACTTGCCAAATCATTAGCAGAAGTTCTTTTTGACAATGAAAAAGCTCTGATTCGTTTTGATATGAGTGAATATATGGAAAAACATTCAGTAGCTAAATTAATTGGTGCACCCCCAGGGTACATAGGTTATGAACAATCAGGTTTGCTAACTGAAGCGGTTAGAAGAAAACCTTATAGCGTCTTGTTATTTGATGAAATTGAAAAGGCACATCCTGATGTAACTAATGTTTTATTACAAGTTTTAGATGATGGTACTTTAAAAGATTCACAAGGAAGGGTTGTTAATTTCAAAAATACTTTGATAATTATGACTTCTAACCTAGGTTCAAATTTTCTTTTAGAAGGAAAAAAAGATTTGGCCATTCAAAGTCTAAAGAAACATTTCCGTCCTGAATTTATAAATCGTATTGATGAGATAGTATTTTTCAATGTTCTTGAGAAAGATACAGTTTTATCGATAATCAACAGCTTGTTGGCACAACTTTCAAAACGCTTGAATAAACAAAATTTATTTTTTAATTTTGATTCAAATCTAACAGAGTTTATCTATAAAAGTAGTTTTGATCAACAGTTTGGTGCAAGACCAATTAAGCGCTTTATTGATCATAGTGTTGCAACTTTAATAGCTAAATATATCCTTCAGGGAAAGATAAAAAAAGGTGTTGGATACAACATTGCAGTTGTTAAAGACAATATTACCATTACACAAAATAATAAGTCTTAA", "aa": "MHLGDEIKKRVKGQDEAIDAVVNTVIRGRVNINDPNKPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKALIRFDMSEYMEKHSVAKLIGAPPGYIGYEQSGLLTEAVRRKPYSVLLFDEIEKAHPDVTNVLLQVLDDGTLKDSQGRVVNFKNTLIIMTSNLGSNFLLEGKKDLAIQSLKKHFRPEFINRIDEIVFFNVLEKDTVLSIINSLLAQLSKRLNKQNLFFNFDSNLTEFIYKSSFDQQFGARPIKRFIDHSVATLIAKYILQGKIKKGVGYNIAVVKDNITITQNNKS", "aa_hexdigest": "2769379323d5850f6787d64dc1c2fdfb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00265DA75C", "query_cov": 0.9659863945578231, "subject_cov": 0.8279883381924198, "identity": 0.5770000000000001, "score": 323.0, "evalue": 3.73e-107, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A661I2M2" }, "genes": [ "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 451613, "stop": 452797, "observations": { "mutation": [ 452794 ], "stop": [ 452794 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47597", "query_cov": 1.0, "subject_cov": 0.4117647058823529, "identity": 0.997, "score": 569.0, "evalue": 1.96e-198, "gene_start": 1, "gene_end": 294, "reference_start": 421, "reference_end": 714, "reference_sequence": "MNINFTPAGENRNFLQEIGRNINDEVLKNKVDPIIGRDNEIRRLIEILSRKSKNNPVLIGEPGVGKTAIVEGFVRRVVSNDVPLNLRDVEIYELSLSGLIAGTKFQGEFEKRINTILKQVKESNGRIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYIEKDGALERRFQKILINEPSSQEALTIMRGLKTRWELFHNITIFDSALVAAVEMSTRYINERNLPDKAIDLIDEAAAKIKTEMSSEPVAIDSLKREIINLETEYAALKQDKENDNKQSKKEYLEKLKKQLDALKQKRDSLINEWKKEKADFENINKLKKEIEEFQTKLETYQSEGNYESASKILYSDIPRLKKELESAQQKYATSKHDLFKTEVSENEIAEVISQTTGIPLKKLLESEKDKLLHLGDEIKKRVKGQDEAIDAVVNTVIRGRVNINDPNKPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKALIRFDMSEYMEKHSVAKLIGAPPGYIGYEQSGLLTEAVRRKPYSVLLFDEIEKAHPDVTNVLLQVLDDGTLKDSQGRVVNFKNTLIIMTSNLGSNFLLEGKKDLAIQSLKKHFRPEFINRIDEIVFFNVLEKDTVLSIINSLLAQLSKRLNKQNLFFNFDSNLTEFIYKSSFDQQFGARPIKRFIDHSVATLIAKYILQGKIKKGVGYNIAVVKDNITITQNNKS" }, "paralog": false, "identity": 0.9974619289340102, "score": 738.0, "evalue": 1.74e-267, "description": "Internal stop codon at 452794. Nonsense mutation around 452794.", "psc": { "uniref90_id": "UniRef90_P47597", "gene": "clpB", "product": "Chaperone protein ClpB", "uniref50_id": "UniRef50_P47597", "cog_id": "COG0542", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47597", "UniRef:UniRef50_P47597", "COG:COG0542", "COG:O", "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ] }, "pscc": { "uniref50_id": "UniRef50_P47597", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47597" ], "product": "Chaperone protein ClpB" } }, "id": "KKNAAMEMLJ_812", "locus": "GCA000027325_00811" }, { "type": "cds", "sequence": "contig_1", "start": 452792, "stop": 453064, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17871.7" ], "nt": "ATGTCAACTCGTTATATTAATGAACGTAATTTACCTGATAAAGCCATTGATCTTATTGATGAGGCTGCTGCTAAGATCAAAACAGAAATGTCATCTGAACCAGTTGCAATAGATAGTCTTAAACGTGAAATAATCAATCTTGAGACAGAGTATGCAGCTCTTAAACAAGATAAGGAAAATGATAACAAACAATCAAAGAAAGAATATTTAGAGAAACTAAAAAAACAATTAGATGCTCTTAAACAAAAGCGTGATTCACTTATAAATGAATGA", "aa": "MSTRYINERNLPDKAIDLIDEAAAKIKTEMSSEPVAIDSLKREIINLETEYAALKQDKENDNKQSKKEYLEKLKKQLDALKQKRDSLINE", "aa_hexdigest": "c2923b5f1575f1c68caf0e08cd475f95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17871.7", "name": "AAA_lid_9", "length": 67, "aa_cov": 0.7444444444444445, "hmm_cov": 1.0, "evalue": 3.727843770945765e-20, "score": 66.9158706665039, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 10451.764399999991, "isoelectric_point": 5.613271141052246 }, "id": "KKNAAMEMLJ_813", "locus": "GCA000027325_00812" }, { "type": "cds", "sequence": "contig_1", "start": 453119, "stop": 453757, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF13191.12", "PFAM:PF01637.24", "PFAM:PF05621.17" ], "nt": "ATGAATATTAATTTCACACCTGCTGGTGAAAATCGTAATTTTTTGCAAGAAATTGGTCGTAATATTAACGATGAAGTATTAAAAAATAAGGTCGATCCTATTATTGGAAGAGATAACGAAATTCGTCGTTTAATTGAGATATTAAGTCGTAAAAGCAAAAACAATCCTGTTTTAATTGGTGAACCTGGAGTTGGTAAAACCGCAATAGTAGAAGGTTTTGTTAGAAGAGTTGTTAGTAATGATGTACCTTTAAATTTAAGGGATGTAGAAATTTATGAACTATCTCTTTCTGGATTAATTGCTGGCACTAAATTCCAAGGTGAATTTGAAAAAAGAATTAATACCATTCTTAAGCAAGTAAAAGAATCAAATGGCAGGATTATTCTTTTTATTGATGAAATTCACCAAATAGTTGGATTAGGACGTAATTCTAGCAGTGGTGCAATGGATATTGCCAATATATTAAAGCCGATGCTAGCTCGAGGAGAAATAAAAGTAATTGGCGCTACTACTCTAAAAGAATACCGGGAATACATTGAAAAAGATGGCGCTTTAGAACGTAGATTTCAAAAAATTCTTATTAACGAGCCTAGTAGTCAAGAGGCACTAACAATTATGCGTGGTTTAAAAACACGTTGA", "aa": "MNINFTPAGENRNFLQEIGRNINDEVLKNKVDPIIGRDNEIRRLIEILSRKSKNNPVLIGEPGVGKTAIVEGFVRRVVSNDVPLNLRDVEIYELSLSGLIAGTKFQGEFEKRINTILKQVKESNGRIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYIEKDGALERRFQKILINEPSSQEALTIMRGLKTR", "aa_hexdigest": "4575529ee5f0e31e572a6ac6ce721931", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001C4E8114", "query_cov": 0.8584905660377359, "subject_cov": 0.9479166666666666, "identity": 0.626, "score": 239.0, "evalue": 1.97e-77, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_J9G8B9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 142, "aa_cov": 0.6367924528301887, "hmm_cov": 0.8732394366197183, "evalue": 4.368663348945362e-18, "score": 61.113277435302734, "start": 57, "stop": 191 }, { "id": "PF13191.12", "name": "AAA_16", "length": 51, "aa_cov": 0.22641509433962265, "hmm_cov": 1.0, "evalue": 1.279644058464101e-10, "score": 37.13175964355469, "start": 34, "stop": 81 }, { "id": "PF01637.24", "name": "ATPase_2", "length": 190, "aa_cov": 0.8066037735849056, "hmm_cov": 0.9947368421052631, "evalue": 7.825735143473433e-11, "score": 37.25398635864258, "start": 37, "stop": 207 }, { "id": "PF05621.17", "name": "TniB", "length": 168, "aa_cov": 0.7311320754716981, "hmm_cov": 0.9702380952380952, "evalue": 7.087279253043963e-11, "score": 36.96131896972656, "start": 40, "stop": 194 } ], "seq_stats": { "molecular_weight": 23865.323100000016, "isoelectric_point": 9.449405097961428 }, "id": "KKNAAMEMLJ_814", "locus": "GCA000027325_00813" }, { "type": "cds", "sequence": "contig_1", "start": 454394, "stop": 454753, "strand": "-", "frame": 2, "gene": "cotS", "product": "putative protein MG356", "db_xrefs": [ "COG:COG0510", "COG:H", "GO:0004305", "GO:0005737", "GO:0006646", "SO:0001217", "UniRef:UniRef50_Q49423", "UniRef:UniRef90_Q49423" ], "nt": "ATGGATTTAAAAAAAGCGGTTGCTTTTCTTTGTGAAAAACTTCAAGTTGTAGAAACAGATATTTTCAAGATAGAGCAAATTCATTCTGGTTTTACTAATTTTTCTTTTTTTTGTGAATTGAAAGATCATAGTAAATTTCAAATAAGACTTTCAAGAAAAGATGTTATTTTAGACAGAAGAAATGAACAAATTATTTTGCAACTAGTAGAACCTTTTTTTTCAAACCCATTTGTTTATTTTGATGTAACAACAGGTAATGCTATAAAAAGGTGGATAGAAGGCACTCAACCTAAAAGAGCAACTGATCTATTTTTATGCAGATTGGTTGAATCTTTAAAAAAAGTTCATTCAGTTGACTGA", "aa": "MDLKKAVAFLCEKLQVVETDIFKIEQIHSGFTNFSFFCELKDHSKFQIRLSRKDVILDRRNEQIILQLVEPFFSNPFVYFDVTTGNAIKRWIEGTQPKRATDLFLCRLVESLKKVHSVD", "aa_hexdigest": "94cdca7201e50f6974daa0173640bf07", "start_type": "ATG", "rbs_motif": null, "genes": [ "cotS" ], "truncated": "5-prime", "pseudogene": { "start": 454091, "stop": 454753, "observations": { "mutation": [ 454192, 454231, 454396 ], "stop": [ 454192, 454231, 454396 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49423", "query_cov": 1.0, "subject_cov": 0.425, "identity": 1.0, "score": 242.0, "evalue": 7.53e-79, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MDLKKAVAFLCEKLQVVETDIFKIEQIHSGFTNFSFFCELKDHSKFQIRLSRKDVILDRRNEQIILQLVEPFFSNPFVYFDVTTGNAIKRWIEGTQPKRATDLFLCRLVESLKKVHSVDWKPFANELLIFDPLVYFEKTKLSAFYKRLYLNLTKKHIDIPKTLCHHDATFDNLVWTPKKQVILIDFEWSCVDNPYYEIANIIREELTIETANKLIDIYGGLKRKLVFETVIYVLLFAFQWTEIMPQTEAILNYRKWVEKRLEYFLKALFPSIWKQAQKNS" }, "paralog": false, "identity": 0.9863636363636363, "score": 435.0, "evalue": 1.69e-157, "description": "Internal stop codon at 454192, 454231, 454396. Nonsense mutation around 454192, 454231, 454396.", "psc": { "uniref90_id": "UniRef90_Q49423", "gene": "CotS", "product": "Uncharacterized protein MG356", "uniref50_id": "UniRef50_Q49423", "cog_id": "COG0510", "cog_category": "H", "go_ids": [ "GO:0005737", "GO:0004305", "GO:0006646" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49423", "UniRef:UniRef50_Q49423", "COG:COG0510", "COG:H", "GO:0005737", "GO:0004305", "GO:0006646" ] }, "pscc": { "uniref50_id": "UniRef50_Q49423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49423" ], "product": "Uncharacterized protein MG356" } }, "id": "KKNAAMEMLJ_815", "locus": "GCA000027325_00814" }, { "type": "cds", "sequence": "contig_1", "start": 454766, "stop": 455278, "strand": "-", "frame": 2, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "GO:0000287", "GO:0005524", "GO:0005737", "GO:0006083", "GO:0006085", "GO:0008776", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_P75245", "UniRef:UniRef90_P47599" ], "nt": "ATGGGATTCACTCCCCTTGAAGGATTAATAATGGGAACACGTAGTGGTGATATTGATCCTGCCATTGTTAGTTACATTGCTGAACAGCAAAAGCTTTCATGTAATGATGTTGTAAATGAATTAAATAAAAAGAGTGGAATGTTTGCTATAACAGGTAGTTCTGACATGCGTGATATTTTTGATAAACCAGAAATTAATGATATTGCTATAAAAATGTATGTTAATCGTGTTGCTGACTATATTGCTAAATACCTAAATCAACTTTCAGGTGAAATTGATAGCTTGGTATTTACTGGGGGAGTTGGTGAAAATGCTAGTTATTGTGTGCAATTAATAATTGAAAAAGTTGCTTCACTTGGTTTTAAAACTAACAGTAATTTATTTGGAAATTATCAAGATAGTTCTCTAATTTCAACAAATGAAAGCAAGTATCAAATTTTTAGAGTTCGTACAAATGAGGAATTGATGATTGTAGAAGATGCTTTGAGAGTAAGTACAAACATTAAAAAATAA", "aa": "MGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK", "aa_hexdigest": "7ec6ac10e44b468ab21afd7c5c0886d4", "start_type": "ATG", "rbs_motif": null, "genes": [ "ackA" ], "truncated": "3-prime", "pseudogene": { "start": 454766, "stop": 455578, "observations": { "mutation": [ 455452 ], "stop": [ 455452 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47599", "query_cov": 1.0, "subject_cov": 0.43256997455470736, "identity": 1.0, "score": 332.0, "evalue": 6.89e-112, "gene_start": 1, "gene_end": 170, "reference_start": 224, "reference_end": 393, "reference_sequence": "MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPENWEKNNLVRRYGFHGTSYKYINEFLEKKFNKKPLNLIVCHLGNGASVCAIKQGKSLNTSMGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK" }, "paralog": false, "identity": 0.9962962962962963, "score": 532.0, "evalue": 3.24e-193, "description": "Internal stop codon at 455452. Nonsense mutation around 455452.", "psc": { "uniref90_id": "UniRef90_P47599", "gene": "ackA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_P75245", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925", "go_ids": [ "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47599", "EC:2.7.2.1", "UniRef:UniRef50_P75245", "COG:COG0282", "COG:C", "KEGG:K00925", "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ] }, "pscc": { "uniref50_id": "UniRef50_P75245", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75245" ], "product": "Acetate kinase" } }, "id": "KKNAAMEMLJ_816", "locus": "GCA000027325_00815" }, { "type": "cds", "sequence": "contig_1", "start": 455450, "stop": 455947, "strand": "-", "frame": 2, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "GO:0000287", "GO:0005524", "GO:0005737", "GO:0006083", "GO:0006085", "GO:0008776", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_P75245", "UniRef:UniRef90_P47599" ], "nt": "ATGCAAAGTCACAAAATCTTGGTTGTTAATGCAGGTAGCAGTTCAATTAAATTTCAACTTTTTAATGATAAAAAACAAGTACTAGCTAAAGGACTTTGTGAACGTATTTTCATTGATGGTTTTTTTAAGCTTGAATTTAATCAAAAAAAGATAGAAGAAAAGGTTCAATTTAATGATCATAATCTTGCTGTTAAGCATTTTTTAAATGCGCTTAAAAAAAACAAAATTATTACTGAACTTTCAGAAATTGGGCTAATAGGGCATAGAGTAGTACAAGGAGCAAATTATTTTACAGATGCAGTTCTTGTTGATACACATTCACTAGCAAAAATAAAAGAATTCATTAAGTTAGCACCGCTTCATAATAAACCAGAAGCAGATGTTATTGAAATTTTTCTAAAAGAGATAAAAACTGCTAAGAATGTTGCTGTATTTGATACCACTTTTCACACTACTATTCCAAGGGAAAATTATCTTTATGCAGTTCCTGAAAATTGA", "aa": "MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPEN", "aa_hexdigest": "74bbfad2789e566929303c95e3086fd4", "start_type": "ATG", "rbs_motif": null, "genes": [ "ackA" ], "truncated": "5-prime", "pseudogene": { "start": 455147, "stop": 455947, "observations": { "mutation": [ 455452 ], "stop": [ 455452 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47599", "query_cov": 1.0, "subject_cov": 0.4198473282442748, "identity": 1.0, "score": 328.0, "evalue": 2.62e-110, "gene_start": 1, "gene_end": 165, "reference_start": 1, "reference_end": 165, "reference_sequence": "MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPENWEKNNLVRRYGFHGTSYKYINEFLEKKFNKKPLNLIVCHLGNGASVCAIKQGKSLNTSMGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK" }, "paralog": false, "identity": 0.9962406015037594, "score": 528.0, "evalue": 1.26e-191, "description": "Internal stop codon at 455452. Nonsense mutation around 455452.", "psc": { "uniref90_id": "UniRef90_P47599", "gene": "ackA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_P75245", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925", "go_ids": [ "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47599", "EC:2.7.2.1", "UniRef:UniRef50_P75245", "COG:COG0282", "COG:C", "KEGG:K00925", "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ] }, "pscc": { "uniref50_id": "UniRef50_P75245", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75245" ], "product": "Acetate kinase" } }, "id": "KKNAAMEMLJ_817", "locus": "GCA000027325_00816" }, { "type": "cds", "sequence": "contig_1", "start": 456055, "stop": 456264, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014894048.1", "SO:0001217", "UniParc:UPI00027B3165", "UniRef:UniRef100_UPI00027B3165", "UniRef:UniRef50_A0AAN4XFQ7", "UniRef:UniRef90_UPI00027B3165" ], "nt": "ATGAAAATTAAAAAATGCTATATTATCGCTGTTATTTCAGCTTTTATTGGTTTTCTTTTTTACTTTTTATCTTCACTTATAGGGATAACAGATGTTAATAATGCAAAAACAGTAGCAACTGTATTTTTTGTATTAGCAACATTAATAGTTGTTAATGCTATTGTCTTCTGGATTCTTTATCTATTCAAAAAAAAGAACGTAGTTAAGTAA", "aa": "MKIKKCYIIAVISAFIGFLFYFLSSLIGITDVNNAKTVATVFFVLATLIVVNAIVFWILYLFKKKNVVK", "aa_hexdigest": "2aec87b6506e412f4fc76993107521a6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3165", "ncbi_nrp_id": "WP_014894048.1", "uniref100_id": "UniRef100_UPI00027B3165", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3165", "RefSeq:WP_014894048.1", "UniRef:UniRef100_UPI00027B3165" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3165", "uniref90_id": "UniRef90_UPI00027B3165" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3165", "uniref50_id": "UniRef50_A0AAN4XFQ7" }, "pscc": { "uniref50_id": "UniRef50_A0AAN4XFQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XFQ7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7823.556999999997, "isoelectric_point": 9.937430763244631 }, "id": "KKNAAMEMLJ_818", "locus": "GCA000027325_00817" }, { "type": "cds", "sequence": "contig_1", "start": 456327, "stop": 456554, "strand": "+", "frame": 3, "gene": null, "product": "MPN534 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75244", "UniRef:UniRef90_UPI0001B417BC" ], "nt": "ATGGAAACTATCAGAGAAACACTTTCTTCTATACAAAGAAGCAATTTTTTATTAAATGAAAGCGCACAAATTCTCAATACGTGTGTAAACAAGAACACATTGCTTGACGATAAGCTGCGCAATTCGATTAATTTTGCACTTAGCATCATTAAGCTAGCAGACTTTTGTCTAAAGGATGATACTGAAAAAATTAATCAATTCTTAGGTAAAACATATGATTTACCATGA", "aa": "METIRETLSSIQRSNFLLNESAQILNTCVNKNTLLDDKLRNSINFALSIIKLADFCLKDDTEKINQFLGKTYDLP", "aa_hexdigest": "db9c1096daeeb392e83726590a16739b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 456327, "stop": 456752, "observations": { "mutation": [ 456552 ], "stop": [ 456552 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B417BC", "query_cov": 1.0, "subject_cov": 0.5319148936170213, "identity": 1.0, "score": 148.0, "evalue": 1.92e-44, "gene_start": 1, "gene_end": 75, "reference_start": 1, "reference_end": 75, "reference_sequence": "METIRETLSSIQRSNFLLNESAQILNTCVNKNTLLDDKLRNSINFALSIIKLADFCLKDDTEKINQFLGKTYDLPWEFQFNQKDKHIECTVKDNFGREKLINLIFQIGDAIETYHTTLIRFKIPKHCLNARDQIKKIMEGK" }, "paralog": false, "identity": 0.9929078014184397, "score": 281.0, "evalue": 1.72e-99, "description": "Internal stop codon at 456552. Nonsense mutation around 456552.", "psc": { "uniref90_id": "UniRef90_UPI0001B417BC", "product": "MPN534 family protein", "uniref50_id": "UniRef50_P75244", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B417BC", "UniRef:UniRef50_P75244" ] }, "pscc": { "uniref50_id": "UniRef50_P75244", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75244" ], "product": "Uncharacterized protein MPN_534" } }, "id": "KKNAAMEMLJ_819", "locus": "GCA000027325_00818" }, { "type": "cds", "sequence": "contig_1", "start": 456872, "stop": 457372, "strand": "+", "frame": 2, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518", "SO:0001217", "UniRef:UniRef50_Q49424", "UniRef:UniRef90_Q49424" ], "nt": "TTGGAAAAAAATAAGCAAATTTTTTGTCATATTATTAAAAAAATTGTCGCTAACCAAATTATAGAAGAGGCTTTTGCCTTTAATACTCTAGAAGAAAAAGAGTGGTTCTGTAGATTAATAGAACTCAATGGTATTGGTAGTAAAACTGCACTTAATTTGCTCAATAATGACCTTGAGGAAATTAAACAATACATTCTGGAAAATAACTACAGTGCATTATGTGGTATTAACGGTGTAAATAACAAAATAGCTCGTGCACTTTTATCACTTGAAATATTTGAAAAATCTGAAAATAATAAAAATATTAAAGGAGTTCAAGTTGCTGATGGTTATGATGAATTGTTTGAAACACTAAAGTCACTTGGTTACAAACAACAAGAAATTCAGGATGCACTAAAAATGATAGAAGTAAAACCTGATTTTGATATAAGTCAGTTAGTTGCAGAAGTAATTAAATTAATGTCTTTTAAGAATAATGAAATTACAAATAAAACCGCCTAA", "aa": "MEKNKQIFCHIIKKIVANQIIEEAFAFNTLEEKEWFCRLIELNGIGSKTALNLLNNDLEEIKQYILENNYSALCGINGVNNKIARALLSLEIFEKSENNKNIKGVQVADGYDELFETLKSLGYKQQEIQDALKMIEVKPDFDISQLVAEVIKLMSFKNNEITNKTA", "aa_hexdigest": "914cde5cfa7e1f2a78d3a2c8ce195e3d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49424", "query_cov": 1.0, "subject_cov": 0.8097560975609757, "identity": 0.9940000000000001, "score": 323.0, "evalue": 4.61e-111, "valid": true, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q49424", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49424" ], "product": "Holliday junction branch migration complex subunit RuvA" }, "genes": [ "ruvA" ], "id": "KKNAAMEMLJ_820", "locus": "GCA000027325_00819" }, { "type": "cds", "sequence": "contig_1", "start": 457347, "stop": 458270, "strand": "+", "frame": 3, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.-", "EC:3.6.4.12", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "RefSeq:WP_009885816.1", "SO:0001217", "UniParc:UPI0000135382", "UniRef:UniRef100_Q49425", "UniRef:UniRef50_Q49425", "UniRef:UniRef90_Q49425" ], "nt": "ATGAAATTACAAATAAAACCGCCTAATACCTTTGATGAATTTGTAGGAAAACAAGAAATAATTAGTCAAATTCAATTAAGTATTAAAGCATCTAAATTAAATAAAACACAACTAGATCATATCTTGTTATATGGCCCACCTGGTGTGGGTAAAACTACTTTAGCCAGATTAATAGCAAATGAATTGAAAACAAAGTTGCAAATTATTCAAGGTGGACATTTACAAAAACCAAGCGATTTCTTAAACGCAATTTCACTCATTAAAAAAGGTGATGTTCTTTTTATAGATGAGATCCATGCCGTAGCACCTAATGTCATGGAACTAATGTATCCAGTTATGGATGTGTTCAAAATACAAGTATTAATTGGCAAGGATTTTAATTCCAAGATAGTTGAAATGAAGGTAAATCCTTTTACTCTAATTGGTGCAACTACACAACTTGGTAAAATCATCAATCCTTTAGAAGATAGATTTGGCGTTATCTTAAACATTAACTATTATTCAAATGCTGAAATTGAAAAGATGGTAAGTATCTATGGAAAGCAAATGAAGTTAGAGCTAAATTCAAATGAAATTTCAGCTATCACTGAACATAGTAAACAAACACCAAGAATTGCAATTAGAATAGTTAGAAGAATATTTGAACAAAAAATTGTTAATAAAAAAATAGACCTTGAGGGTTTGTTTAAGAATTTAATGATTTATAAAAATGGTCTGCAAAGTATTGATGTCCAATATCTTGAGGTTTTAAATCGCCAAAATGAACCACAAGGAATTAAGTCAATTAGTTCCATGTTAGGTATAGACAGACACACTATAGAAAATAAAATTGAACCTTTTTTGTTGCGTGAAAATATGATTCAAAAAACCAAAAAAGGCAGGATTATTACAAATAGCGGAAGAGAATATTTAGTTAACTTTTAA", "aa": "MKLQIKPPNTFDEFVGKQEIISQIQLSIKASKLNKTQLDHILLYGPPGVGKTTLARLIANELKTKLQIIQGGHLQKPSDFLNAISLIKKGDVLFIDEIHAVAPNVMELMYPVMDVFKIQVLIGKDFNSKIVEMKVNPFTLIGATTQLGKIINPLEDRFGVILNINYYSNAEIEKMVSIYGKQMKLELNSNEISAITEHSKQTPRIAIRIVRRIFEQKIVNKKIDLEGLFKNLMIYKNGLQSIDVQYLEVLNRQNEPQGIKSISSMLGIDRHTIENKIEPFLLRENMIQKTKKGRIITNSGREYLVNF", "aa_hexdigest": "029e8b1d2ad1ef50aa3ed7b7ed65658a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135382", "ncbi_nrp_id": "WP_009885816.1", "uniref100_id": "UniRef100_Q49425", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135382", "RefSeq:WP_009885816.1", "UniRef:UniRef100_Q49425" ] }, "ips": { "uniref100_id": "UniRef100_Q49425", "uniref90_id": "UniRef90_Q49425" }, "psc": { "uniref90_id": "UniRef90_Q49425", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.-", "3.6.4.12" ], "uniref50_id": "UniRef50_Q49425", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49425" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "KKNAAMEMLJ_821", "locus": "GCA000027325_00820" }, { "type": "cds", "sequence": "contig_1", "start": 458260, "stop": 458547, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATATTCAAACGATTATAGAAAACTACTGTCAATAACAAAACGATTATTCAACAGACTATTAATTAATACTGAAAAGAACGTTCGATTAATAGGTATTAGTTTTTTTGATTTAAAAAAGATAGATACAGATGAAGGACAGAAAAAATCTTTATTTTATCAATTCATACCAAAATCAATTAGCAAATTAAGCGAAGAATCTTCACTTGATAAATTAATTTTTGATATTAATGAAAGTTTTGGTTTTGAAATTATTAAAAGAGCTAATAAGCTTAAAAGTTAA", "aa": "MKKYSNDYRKLLSITKRLFNRLLINTEKNVRLIGISFFDLKKIDTDEGQKKSLFYQFIPKSISKLSEESSLDKLIFDINESFGFEIIKRANKLKS", "aa_hexdigest": "187c5e3628d36d6c5a42578e9c0f0fd3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11227.981499999989, "isoelectric_point": 9.800693321228025 }, "id": "KKNAAMEMLJ_822", "locus": "GCA000027325_00821" }, { "type": "cds", "sequence": "contig_1", "start": 458932, "stop": 459495, "strand": "-", "frame": 3, "gene": "dinP", "product": "putative protein MG360", "db_xrefs": [ "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0009432", "GO:0042276", "SO:0001217", "UniRef:UniRef50_P75241", "UniRef:UniRef90_Q49426" ], "nt": "ATGATTAACACCTTTACATATTTTGAACCAGAATATTTAATTGATAAAAATTTAATTTTTCTTTATTTTGATTTTGATGCTTTTTTTGCATCTGTTGAAGAATTAGAAAATCCAGAATTAGTTAATCAACCCTTAATAGTTGGTAATCGCTTTTCAAGAAGTGTAGTTTCAACTTGTAACTATGTTGCTCGTAGTTATGGAATTAGATCTGGAATGTCTATTTTAAAAGCATTAGAACTATGTCCAAATGCTATTTTTGCACATTCTAATTTTCGTAATTACAGAAAACATTCTAAACGTATTTTTTCAGTTATTGAAAGCACTTTTTCATTAAAAATTGATGTTCTTTCTGTTGATGAAGGGGTTGCTTGTTTTCAAAACATTTCATTTAAAAAGGCATTTTTAATAGCGAAAAAAATAAAAAATTTTGTTTTTCAAAACTTGAGAATCAAAATTTCAATTGGCATCTCAGATCATTTTTTAATTGCTAAGATTTTTTCAAATCAAGCAAAACCATTTGGAATTAAAAGTTGTTCAGTTAAGGATATTAAAAAAAAACTTTGA", "aa": "MINTFTYFEPEYLIDKNLIFLYFDFDAFFASVEELENPELVNQPLIVGNRFSRSVVSTCNYVARSYGIRSGMSILKALELCPNAIFAHSNFRNYRKHSKRIFSVIESTFSLKIDVLSVDEGVACFQNISFKKAFLIAKKIKNFVFQNLRIKISIGISDHFLIAKIFSNQAKPFGIKSCSVKDIKKKL", "aa_hexdigest": "d6153e2b814a3478ebb588cb1d487661", "start_type": "ATG", "rbs_motif": null, "genes": [ "dinP" ], "truncated": "5-prime", "pseudogene": { "start": 458629, "stop": 459495, "observations": { "mutation": [ 458763, 458796, 458934 ], "stop": [ 458763, 458796, 458934 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49426", "query_cov": 1.0, "subject_cov": 0.45498783454987834, "identity": 1.0, "score": 367.0, "evalue": 5.43e-125, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MINTFTYFEPEYLIDKNLIFLYFDFDAFFASVEELENPELVNQPLIVGNRFSRSVVSTCNYVARSYGIRSGMSILKALELCPNAIFAHSNFRNYRKHSKRIFSVIESTFSLKIDVLSVDEGVACFQNISFKKAFLIAKKIKNFVFQNLRIKISIGISDHFLIAKIFSNQAKPFGIKSCSVKDIKKKLWPLPITEIPGIGEKHIDLVFKNNFYKINDLAVCEDASLLKKVFGNFWESLKAVSLGKWYTDNNNQVKSRSFAVSETLEDLNYSNNQLNKKLTQIFDQLFIRLQLSSQVCKGIVVQLKSNDFIVNSHSNKMKKYSNDYRKLLSITKRLFNRLLINTEKNVRLIGISFFDLKKIDTDEGQKKSLFYQFIPKSISKLSEESSLDKLIFDINESFGFEIIKRANKLKS" }, "paralog": false, "identity": 0.9895833333333334, "score": 558.0, "evalue": 5.44e-203, "description": "Internal stop codon at 458763, 458796, 458934. Nonsense mutation around 458763, 458796, 458934.", "psc": { "uniref90_id": "UniRef90_Q49426", "gene": "DinP", "product": "Uncharacterized protein MG360", "uniref50_id": "UniRef50_P75241", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49426", "UniRef:UniRef50_P75241", "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75241", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75241" ], "product": "Uncharacterized protein MG360 homolog" } }, "id": "KKNAAMEMLJ_823", "locus": "GCA000027325_00822" }, { "type": "cds", "sequence": "contig_1", "start": 459615, "stop": 460103, "strand": "+", "frame": 3, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02864", "RefSeq:WP_009885818.1", "SO:0001217", "UniParc:UPI0000133B39", "UniRef:UniRef100_P36263", "UniRef:UniRef50_P36263", "UniRef:UniRef90_P36263" ], "nt": "ATGGTAGATAGTAAGAAAAATAAAAAACAGCAGGTTACGGATTTTTCTAATTTACTCTCTCAAAGTAAAGGATTTGTTATTTTTGACTATTCAGGAATGTCTGCTGTTGATGCAACTTTAATGAGAAAAAAGTTGTTTAATAAGGGTAGTAAGATAAAAATTGTTAAAAACAATATCTTAAGACGTGCTTTAAAAACTAGTAATTTTGAAGGTGTTGATGAATCGGTCATCAAAGGAAAAATTGCAGTTGCTGTTGGTATTAACGAGATCTTAGAAACCTTAAAAGTTGTTGATAGTGTAGTTAAAGAAAAAGAGTTAATGAAATTTGTTTGTGGTCATTTTGATAACCGTATTTTTAATAGTGATGACTTACAAAAAATAGCAAAACTCCCTGGTAGAAATGAACTTTATGGAATGTTTCTTTCAGTTCTACAAGCACCATTACGAAAATTTCTCTATGCTCTTCAGGCAGTAAGGAATGCTAAGTAA", "aa": "MVDSKKNKKQQVTDFSNLLSQSKGFVIFDYSGMSAVDATLMRKKLFNKGSKIKIVKNNILRRALKTSNFEGVDESVIKGKIAVAVGINEILETLKVVDSVVKEKELMKFVCGHFDNRIFNSDDLQKIAKLPGRNELYGMFLSVLQAPLRKFLYALQAVRNAK", "aa_hexdigest": "4d14f11ca59c4d52f196969e98ce2e5e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133B39", "ncbi_nrp_id": "WP_009885818.1", "uniref100_id": "UniRef100_P36263", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133B39", "RefSeq:WP_009885818.1", "UniRef:UniRef100_P36263" ] }, "ips": { "uniref100_id": "UniRef100_P36263", "uniref90_id": "UniRef90_P36263" }, "psc": { "uniref90_id": "UniRef90_P36263", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_P36263", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P36263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36263" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "KKNAAMEMLJ_824", "locus": "GCA000027325_00823" }, { "type": "cds", "sequence": "contig_1", "start": 460126, "stop": 460494, "strand": "+", "frame": 1, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_009885819.1", "SO:0001217", "UniParc:UPI00001341B3", "UniRef:UniRef100_P36255", "UniRef:UniRef50_P36255", "UniRef:UniRef90_P36255" ], "nt": "ATGGGAAAACTAGATAAAAAACAATTAATTGAATCTCTAAAAGAGATGACTATAGTTGAAATTGATGAAATAATCAAGGCTGTTGAAGAAGCATTTGGTGTAACTGCAACTCCAATAGTAGCTGCTGGCGCAGCTGGTGCTACACAAGAAGCTGCTAGCGAAGTTAGTGTAAAGGTAACAGGATATGCTGATAATGCTAAGTTAGCTGTTTTAAAACTTTATCGTGAAATTACTGGAGTTGGTTTAATGGAAGCTAAAACTGCAGTTGAAAAATTACCTTGCGTTGTAAAACAAGATATTAAACCAGAAGAAGCAGAAGAACTTAAAAAGCGTTTTGTTGAAGTTGGTGCAACTGTTGAAGTTAAATAA", "aa": "MGKLDKKQLIESLKEMTIVEIDEIIKAVEEAFGVTATPIVAAGAAGATQEAASEVSVKVTGYADNAKLAVLKLYREITGVGLMEAKTAVEKLPCVVKQDIKPEEAEELKKRFVEVGATVEVK", "aa_hexdigest": "2a89c681ac8f1bc6764b695478e7c7bb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001341B3", "ncbi_nrp_id": "WP_009885819.1", "uniref100_id": "UniRef100_P36255", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341B3", "RefSeq:WP_009885819.1", "UniRef:UniRef100_P36255" ] }, "ips": { "uniref100_id": "UniRef100_P36255", "uniref90_id": "UniRef90_P36255" }, "psc": { "uniref90_id": "UniRef90_P36255", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_P36255", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P36255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36255" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "KKNAAMEMLJ_825", "locus": "GCA000027325_00824" }, { "type": "cds", "sequence": "contig_1", "start": 460497, "stop": 460670, "strand": "+", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_009885820.1", "SO:0001217", "UniParc:UPI0000165F83", "UniRef:UniRef100_P47603", "UniRef:UniRef50_P47603", "UniRef:UniRef90_P47603" ], "nt": "ATGGCAGTACAACAACGGCGTTCTAGTAAACACCGTCGTGATAAAAGACGTTCTCACGATGCACTTACTCTACAAACTTTAAGTGTTTGTAAGAAATGTGGTAAGAAGAAGTTATCACATCGTGTGTGCTCTTGTGGTATGTACGGTGAACTAAGAGTTAAAAAAGCTCACTAA", "aa": "MAVQQRRSSKHRRDKRRSHDALTLQTLSVCKKCGKKKLSHRVCSCGMYGELRVKKAH", "aa_hexdigest": "cf34ba30d94ac5e4214a0fce93736145", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F83", "ncbi_nrp_id": "WP_009885820.1", "uniref100_id": "UniRef100_P47603", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F83", "RefSeq:WP_009885820.1", "UniRef:UniRef100_P47603" ] }, "ips": { "uniref100_id": "UniRef100_P47603", "uniref90_id": "UniRef90_P47603" }, "psc": { "uniref90_id": "UniRef90_P47603", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_P47603", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_P47603", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47603" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "KKNAAMEMLJ_826", "locus": "GCA000027325_00825" }, { "type": "cds", "sequence": "contig_1", "start": 460685, "stop": 460951, "strand": "-", "frame": 2, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02968", "RefSeq:WP_009885821.1", "SO:0001217", "UniParc:UPI0000134E49", "UniRef:UniRef100_P55750", "UniRef:UniRef50_P55750", "UniRef:UniRef90_P55750" ], "nt": "ATGGCTAATATTAAATCTAACGAAAAACGATTACGTCAAGACATTAAGAGAAATTTAAATAATAAAGGACAAAAAACTAAACTAAAAACTAATGTTAAAAAATTTAATAAAGAGATTAATTTAGATAATCTCAGTTCTGTTTATTCTCAAGCAGATCGTTTAGCCAGAAAAGGGATTATTTCTTTAAACAGAGCTAAGCGTTTAAAATCAAAAAATGCTGTTATTTTGCATAAAAGTAATACAAATTCAACTGCAAAAAAACAATAA", "aa": "MANIKSNEKRLRQDIKRNLNNKGQKTKLKTNVKKFNKEINLDNLSSVYSQADRLARKGIISLNRAKRLKSKNAVILHKSNTNSTAKKQ", "aa_hexdigest": "5720b3c8f1e572f2bd77a0be8e9a894d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E49", "ncbi_nrp_id": "WP_009885821.1", "uniref100_id": "UniRef100_P55750", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E49", "RefSeq:WP_009885821.1", "UniRef:UniRef100_P55750" ] }, "ips": { "uniref100_id": "UniRef100_P55750", "uniref90_id": "UniRef90_P55750" }, "psc": { "uniref90_id": "UniRef90_P55750", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_P55750", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P55750", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55750" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "KKNAAMEMLJ_827", "locus": "GCA000027325_00826" }, { "type": "cds", "sequence": "contig_1", "start": 461015, "stop": 461170, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGGTGGACAACAAGGGAGTTTTTTTGGGCTTTTAGTAATTGTTATTCCAATAATTTTGTTAATTGTTTTTTTTTCCAAGAAAAAAGGGGCACAAAAAAATGATTTTAGTGGTGAAGGAGGTAATCGATCATCAAGAAAAGATGAAGTGTGA", "aa": "MDGGQQGSFFGLLVIVIPIILLIVFFSKKKGAQKNDFSGEGGNRSSRKDEV", "aa_hexdigest": "c9c382566edc608eb2106c3ee9515271", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5529.328600000002, "isoelectric_point": 9.397443580627442 }, "id": "KKNAAMEMLJ_828", "locus": "GCA000027325_00827" }, { "type": "cds", "sequence": "contig_1", "start": 461189, "stop": 461545, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604", "UniRef:UniRef90_P47604" ], "nt": "TTGCAAGAAAAGAATGAACGTGGTAAAGAAATTATTAAAACTTTTGTAGCTAAAAAACCAAACCCTTTACATTCAAAAAAAGACCGCAAGCTTTTCAATCAAGAGATACAGGCATATATTACTAGTAATAACTTAGGAAAAAGCGAAGCAAAACGTTATAAAAATGAGCAAACTCGCTTAATGCAAAGAGAACTTTATTGTATTTATTTTGTTACAAAAGATGCTAAATCAACTGAAGTTGATGATGCTAGGATTATAGAAGCTGAGGTTTATCAAAAACCTACAAAAACCAAAAGTACTCCAGAGCGGCTAATTCGTATACTTGGTTTAAAAAATTTTGAAACTGAAATGCAATGA", "aa": "MQEKNERGKEIIKTFVAKKPNPLHSKKDRKLFNQEIQAYITSNNLGKSEAKRYKNEQTRLMQRELYCIYFVTKDAKSTEVDDARIIEAEVYQKPTKTKSTPERLIRILGLKNFETEMQ", "aa_hexdigest": "1f6d90f48589cd81fa683cff549a11aa", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 461015, "stop": 461689, "observations": { "mutation": [ 461168, 461543 ], "stop": [ 461168, 461543 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47604", "query_cov": 1.0, "subject_cov": 0.5267857142857143, "identity": 0.992, "score": 228.0, "evalue": 5.04e-74, "gene_start": 1, "gene_end": 118, "reference_start": 59, "reference_end": 176, "reference_sequence": "MDGGQQGSFFGLLVIVIPIILLIVFFSKKKGAQKNDFSGEGGNRSSRKDEVWKTIKQFLQEKNERGKEIIKTFVAKKPNPLHSKKDRKLFNQEIQAYITSNNLGKSEAKRYKNEQTRLMQRELYCIYFVTKDAKSTEVDDARIIEAEVYQKPTKTKSTPERLIRILGLKNFETEMQWIQPLMVREEKRKEKEEQKKLKLAARELKKKKKKKIKKPKEIRNQKNV" }, "paralog": false, "identity": 0.9866071428571429, "score": 341.0, "evalue": 2.88e-121, "description": "Internal stop codon at 461168, 461543. Nonsense mutation around 461168, 461543.", "psc": { "uniref90_id": "UniRef90_P47604", "product": "Uncharacterized protein MG364", "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47604", "UniRef:UniRef50_P47604" ] }, "pscc": { "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604" ], "product": "Uncharacterized protein MG364" } }, "id": "KKNAAMEMLJ_829", "locus": "GCA000027325_00828" }, { "type": "cds", "sequence": "contig_1", "start": 461558, "stop": 461689, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCGTGAAGAAAAGAGAAAAGAAAAAGAAGAACAGAAAAAACTTAAATTAGCTGCAAGAGAACTAAAAAAGAAGAAAAAGAAGAAAATAAAAAAACCAAAAGAAATCAGAAATCAGAAAAATGTTTAA", "aa": "MVREEKRKEKEEQKKLKLAARELKKKKKKKIKKPKEIRNQKNV", "aa_hexdigest": "fb7dc0fd268b06683a3ce1714668d93a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5344.465800000003, "isoelectric_point": 10.49488887786865 }, "id": "KKNAAMEMLJ_830", "locus": "GCA000027325_00829" }, { "type": "cds", "sequence": "contig_1", "start": 461682, "stop": 462047, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00551.24" ], "nt": "ATGTTTAAAATTGTTTTCTTTGGTACTTCAACGCTTTCAAAAAAATGTTTAGAACAACTTTTTTACGATAATGATTTTGAAATTTGTGCTGTTGTAACTCAGCCAGACAAAATTAATCATCGTAACAATAAAATAGTACCTTCTGATGTTAAGTCTTTTTGTTTGGAAAAAAACATAACTTTTTTTCAACCAAAACAAAGCATAAGCATAAAAGCTGATCTAGAAAAATTAAAAGCTGATATTGGTATTTGCGTTTCATTTGGTCAGTATCTTCATCAAGATATTATTGATCTTTTTCCAAATAAAGTAATTAACTTACATCCTTCTAAGTTACCACTACTTCGTGGTGGTGCACCATTACATTGA", "aa": "MFKIVFFGTSTLSKKCLEQLFYDNDFEICAVVTQPDKINHRNNKIVPSDVKSFCLEKNITFFQPKQSISIKADLEKLKADIGICVSFGQYLHQDIIDLFPNKVINLHPSKLPLLRGGAPLH", "aa_hexdigest": "905fc30a3e5ee055ff22e215af100c8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00551.24", "name": "Formyl_trans_N", "length": 123, "aa_cov": 0.9752066115702479, "hmm_cov": 0.975609756097561, "evalue": 1.3051956945807554e-17, "score": 59.202640533447266, "start": 3, "stop": 120 } ], "seq_stats": { "molecular_weight": 13764.949899999981, "isoelectric_point": 8.693255424499512 }, "id": "KKNAAMEMLJ_831", "locus": "GCA000027325_00830" }, { "type": "cds", "sequence": "contig_1", "start": 462619, "stop": 463560, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031D7E73", "UniRef:UniRef90_UPI00031D7E73" ], "nt": "ATGATTACAAAGAGTTTTTTCCTTAAAAATTTTGATCGCTCTAAAGAATTAATTCCTCTTTCATATAACGATGTTTTTAGCGCTGTTAATCAGTTTCTGAAAAGCTATACTGACGTTAATGATATTGATATTATTGAAGAAAATTTGATTGAAGATCCAGTTTTTGAACTTGATGTTTTAGAACTTTTAAACGAGGATCCAATGCTATTATTGGACAGTAAAAATCCCCGTGTTCAAGAAGCTAAAATTATTGCTAATAAAGCAAAAAAAAATATTAAAGATTATTTTAATCTTCCCATTTTTTTTGATACAGATAGTTTAGATAAAAATGTTTCTGTTTATCAAAAAGCTGAGCTTACTGAAAAGAAAATTCAAGAAATAATAACATCAAAAAAATCAGCTATTATTTTTAAACCTATTTTTGAAATAGAAGATTGCTTAATTCAACCAGATGCTATTATTGTTCATGAAAAAGGACTTTGTGAATTTGTTGTTATAAAAGCTACAACCAATACAAAAAGAAAATATTTTTTGGAAATAATTTATGACTTTGTGTTATTTAAAAAAATAGGTAAGTATAAACTTTTAAACTATTATTTTTGTACTGTTAAATACGAATTACAAAATAAAAATAATGTTTCTTTCTTTTTAAATACTGAAATAAAAACATCAAAAAACAGTTTTAGTTTAAGTTCAAAAGAAAAAGATTATTTTAAGAATAAACCTTTTAATCACCCTGAAAAAATTGCTTACATACACAAAAAAAAGAGCAATGGAGTTAATGGTTTTTTGATTGTAAAACTCATTGATAACCTTATTAAAAACAATATAGTTGATTTAAACAAAATAAGTGATTTTGTTACTAAAGAAATTGACAGTAAAAGTGTTCGTAATATTCAACCACTTATTAAAAATGCGGCTAAAATACAAATTAATTTTTGA", "aa": "MITKSFFLKNFDRSKELIPLSYNDVFSAVNQFLKSYTDVNDIDIIEENLIEDPVFELDVLELLNEDPMLLLDSKNPRVQEAKIIANKAKKNIKDYFNLPIFFDTDSLDKNVSVYQKAELTEKKIQEIITSKKSAIIFKPIFEIEDCLIQPDAIIVHEKGLCEFVVIKATTNTKRKYFLEIIYDFVLFKKIGKYKLLNYYFCTVKYELQNKNNVSFFLNTEIKTSKNSFSLSSKEKDYFKNKPFNHPEKIAYIHKKKSNGVNGFLIVKLIDNLIKNNIVDLNKISDFVTKEIDSKSVRNIQPLIKNAAKIQINF", "aa_hexdigest": "2e1dfd7af8b61c3537278b86f29a960c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 462619, "stop": 463863, "observations": { "mutation": [ 463558, 463735 ], "stop": [ 463558, 463735 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00031D7E73", "query_cov": 1.0, "subject_cov": 0.6879120879120879, "identity": 0.997, "score": 597.0, "evalue": 6.9e-213, "gene_start": 1, "gene_end": 313, "reference_start": 1, "reference_end": 313, "reference_sequence": "MITKSFFLKNFDRSKELIPLSYNDVFSAVNQFLKSYTDVNDIDIIEENLIEDPVFELDVLELLNEDPMLLLDSKNPRVQEAKIIANKAKKNIKDYFNLPIFFDTDSLDKNVSVYQKAELTEKKIQKIITSKKSAIIFKPIFEIEDCLIQPDAIIVHEKGLCEFVVIKATTNTKRKYFLEIIYDFVLFKKIGKYKLLNYYFCTVKYELQNKNNVSFFLNTEIKTSKNSFSLSSKEKDYFKNKPFNHPEKIAYIHKKKSNGVNGFLIVKLIDNLIKNNIVDLNKISDFVTKEIDSKSVRNIQPLIKNAAKIQINFWDQIQDIKKYQELKINQIVFNYSENFDSFWSNYLLRNLIKLVFAHKYNEIFKLSGKLANWSQLTYAYKENKSITINQLLHELNQKKSKANFNNSTNKISFFLEAWNSEKGFAIGNKFKNTWNKLKKRKFILILKQLVHQSES" }, "paralog": false, "identity": 0.9927536231884058, "score": 809.0, "evalue": 3.11e-299, "description": "Internal stop codon at 463558, 463735. Nonsense mutation around 463558, 463735.", "psc": { "uniref90_id": "UniRef90_UPI00031D7E73", "uniref50_id": "UniRef50_UPI00031D7E73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00031D7E73", "UniRef:UniRef50_UPI00031D7E73" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00031D7E73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031D7E73" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 36505.9634, "isoelectric_point": 8.857198524475098 }, "id": "KKNAAMEMLJ_832", "locus": "GCA000027325_00831" }, { "type": "cds", "sequence": "contig_1", "start": 464215, "stop": 464496, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACATTGATTAATGAACAAATATATAAAGAAAAAGTAAAAGCTATTGTTGACAATCTTTTTGACTTAGCTGATATTTTTACTATTGAAAATAACTGTTTGGCTTTCAAACAGCTAAACGGTTTTTCCTCTATCAAAAAGGTTTTAACTATTATAGATGAAAGCTTTTTAAAGGCAAGTAAATCAATTGGTTATCAAAATTTAAAAATCCAAAAAGGTGATGTAGCACAAGAAGTTGCTTTATCGCGTTTTCTAAATTGCTTAAATAAAAATGAATGA", "aa": "MATLINEQIYKEKVKAIVDNLFDLADIFTIENNCLAFKQLNGFSSIKKVLTIIDESFLKASKSIGYQNLKIQKGDVAQEVALSRFLNCLNKNE", "aa_hexdigest": "02ef597de60abf94d47f042022153718", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10515.09699999999, "isoelectric_point": 7.716768455505371 }, "id": "KKNAAMEMLJ_833", "locus": "GCA000027325_00832" }, { "type": "cds", "sequence": "contig_1", "start": 464646, "stop": 464768, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTCTTTTACAAGTTTTTTAAAAAGTAGTAAAGGAAGCCATTTTTTTAGTGATTTAAAAGAGAAGATAGAAAATCAAAAGATGTGTAAGAAACTAGCTATTAAACCTAAAAAAAATTAG", "aa": "MASFTSFLKSSKGSHFFSDLKEKIENQKMCKKLAIKPKKN", "aa_hexdigest": "57e6d5bc23b1c41667eb7dd61ca46cd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4606.4355000000005, "isoelectric_point": 10.015501976013184 }, "id": "KKNAAMEMLJ_834", "locus": "GCA000027325_00833" }, { "type": "cds", "sequence": "contig_1", "start": 464829, "stop": 465125, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCTAAAATTAGGTGATTTTGTTAAGTTAAGCAATGGTGCTGAACTAACTGAAAACACTGTTGGTGATGTACTTGAAGCTTTGGTTGGCGCCATTTATGAAGATATGGGGATGAAAAAAGCAACTGAATTTGTTGAAAAATATATTTTTGAAAGAACTTTTTCTGAGATTTTAAAATACGATTTCTTTTCACTTTTTCAAGAGCAAAAATTACCTGAACCAAGAGTTAGGGTAAGCTTAACTTCAAATAATTTGGTACTTAGTATAATTGAACTTGATGGTGATATTATTTGA", "aa": "MELKLGDFVKLSNGAELTENTVGDVLEALVGAIYEDMGMKKATEFVEKYIFERTFSEILKYDFFSLFQEQKLPEPRVRVSLTSNNLVLSIIELDGDII", "aa_hexdigest": "df634c168a522092a11f0653ccece72c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11154.69699999999, "isoelectric_point": 4.43170223236084 }, "id": "KKNAAMEMLJ_835", "locus": "GCA000027325_00834" }, { "type": "cds", "sequence": "contig_1", "start": 465424, "stop": 466410, "strand": "-", "frame": 3, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811", "KEGG:K03621", "RefSeq:WP_009885824.1", "SO:0001217", "UniParc:UPI0000131C65", "UniRef:UniRef100_Q49427", "UniRef:UniRef50_Q49427", "UniRef:UniRef90_Q49427" ], "nt": "ATGGCTTTTAGATTTGCAGTTGATTGTTTAGGTTTTGAAAACAAACCTAGTGAAGCAATTGAAGCAGTATTGAAATATTGGAGTTTTCATCAGGACTTAAATTTCATTCTGATCGGTGATGAAAAGGCTTTTGATGGTCTTGATATACTTCCAAAAAATATTACAAAAAAACTTGCTAATTCTTTCATTGAAATGACCGACACTCCACTAAGTGCAAGAAGAAAAGTTAACAGTTCAATGCAAATAGCCATAAACTTAGTTCGTGAAGGTAATGCTGATGTTGTAATTTCAGCAGGCTCTTCAGCAGTTTATGCTTCTTTAACAAATGATGCTTTTGGCAAAATTAATAAAAATACTAAGAGTGCTTTTATGTCTTATGTACCGACAGATAACAATAATTGGTTTTATTTTTTGGATGTTGGAGCTAATAAATATTTCACAGGAAAAGAACTTTATTTTTTAGGTTTAATGGCTGATATTTTTGTTAAAAAAACAACTACAAAAAAAACTCCTAAAATTGGACTATTGAATATAGGAACAGAAGAAAATAAAGGTTTTGATTATCATCAAGAAGCATTTAAGCTTTTAAAAGCTGATAAAAATCTTAACTTTTTAGGATTTGTGGAATCAAGGTTTTTACTGGATGGAATATGTGATATTTTGATAGCTGATGGATATAGCGGCAATTTAGTTTTAAAAGCAATGGAAGGTACTTTTAAAACGATAGCACGGATTCTTAAGAGGGGTTATAAAAGAAATCCTCTTGCTGGCTTATTTAGCTTAGGTATTATCAAAAGTGTAGCCAAAAAATTTGATTACAAAAATAACGCTGGAGCTGTTGTAATGGGTTTAAATAAATTAGCTTTAAAAACTCACGGTTCTGCTGATAAACAACAATTTTTAAGCACGATTAGATTAGCACATCTAAGCTTAAAATCTGATCTTATTAATGCAATTAAAACTTCTTTGAATAACTATGAAGAATAA", "aa": "MAFRFAVDCLGFENKPSEAIEAVLKYWSFHQDLNFILIGDEKAFDGLDILPKNITKKLANSFIEMTDTPLSARRKVNSSMQIAINLVREGNADVVISAGSSAVYASLTNDAFGKINKNTKSAFMSYVPTDNNNWFYFLDVGANKYFTGKELYFLGLMADIFVKKTTTKKTPKIGLLNIGTEENKGFDYHQEAFKLLKADKNLNFLGFVESRFLLDGICDILIADGYSGNLVLKAMEGTFKTIARILKRGYKRNPLAGLFSLGIIKSVAKKFDYKNNAGAVVMGLNKLALKTHGSADKQQFLSTIRLAHLSLKSDLINAIKTSLNNYEE", "aa_hexdigest": "7153b7d2f88026ae33926ff1dfa6f4e8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000131C65", "ncbi_nrp_id": "WP_009885824.1", "uniref100_id": "UniRef100_Q49427", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000131C65", "RefSeq:WP_009885824.1", "UniRef:UniRef100_Q49427" ] }, "ips": { "uniref100_id": "UniRef100_Q49427", "uniref90_id": "UniRef90_Q49427" }, "psc": { "uniref90_id": "UniRef90_Q49427", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q49427", "cog_id": "COG0416", "cog_category": "I", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q49427", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49427" ], "product": "Phosphate acyltransferase" }, "genes": [ "plsX" ], "id": "KKNAAMEMLJ_836", "locus": "GCA000027325_00835" }, { "type": "cds", "sequence": "contig_1", "start": 466410, "stop": 467564, "strand": "-", "frame": 1, "gene": "fakA", "product": "putative protein MG369", "db_xrefs": [ "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071", "SO:0001217", "UniRef:UniRef50_P75231", "UniRef:UniRef90_P47609" ], "nt": "GTGAAAAAAACTCCCCAAATGTTACCTGTATTAAAAGCATCAGGTGTGGTTGATTCAGGTGCTTATGGTTTTGCTTGTTTTCTTGAAGGAATGTTGTCATATTATGGGGGCGAATCTAATTTAGATGACAATACTTTATCAACTATAGAAATTAAATTTAACAAGTTTAAAGAACAACATGCTAATGAAGATGAATTTGGTTATTGTACAGAATATGTTTTAAGACTAGGTTTAAAAATAAATCAGACAGTTGAAAAGCAAAAATTTCATCAAAAAAAGTTTGAAAGTAAAGTAAATAGAATTGCCAATTCTGTAGTAATTGCTAGTGATAAAGACAATGGTTTTGTTAAAGTTCATGCTCATACTTTAAAACCACATCTTTTATTAGAAATGGGTCTTAATTATGGTGAATTTGAATTTGTCAAAATTGATAACATGAATTTACAGGTTAATAACAAGAATAACTCACCAACAAAAAGAGTTTTAAAACCTGCTATAGTAGCTACTGTACCTACAGAAGCATTTGCTGAAAGAATTCGTGAAGATCATGATATTAACGCCATATTATGTACTGATGATACTGGGGCTCCTTCTGTTTTTTCTTTACTTGAGGCTGTTAAGTTAACCAATTCTAGTAATGTAATCTTTTTGTTACATGATAAAAACTATTTTCTATCTGCCAATGAAACAATTAAACAGTTAAAACACCAAAAGATTAATGCAGATTATGTTATTACAGCAAATCCTGTGGAGTCAATTGCTGCTTTAACTGTATTTAACAGTGATCTTTCTATTCACACTAATGTGAAAGCAATGAAGCGCTTTATTAAAGAGTTTGCTTCCGCTACGATAACACAAGCTTCAAAGTCATATAAAGAAAACAAAGTAATGGTGAATAAAAATGATTTTATTGCTGTTACAAATAAAAGTATTATTGCTAGTGAATCTCAACTTACAGACTGTTTTTTTAAAACAATTGATATCCTTTCAAAAAAAGTGAAAAAACCTGAGTTCCTTTTGGCTTATTATGGCAAAGACATCACTGAACAAGATGCGAAAAAAATGCAAGCTTTAGTTGAAAAAAAATATAAATTGTTTTGTGAATTTTCACCTGGAGAACAAAAAGTGTTCTCTTACATAATAGGCATACAGTAA", "aa": "MKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSYYGGESNLDDNTLSTIEIKFNKFKEQHANEDEFGYCTEYVLRLGLKINQTVEKQKFHQKKFESKVNRIANSVVIASDKDNGFVKVHAHTLKPHLLLEMGLNYGEFEFVKIDNMNLQVNNKNNSPTKRVLKPAIVATVPTEAFAERIREDHDINAILCTDDTGAPSVFSLLEAVKLTNSSNVIFLLHDKNYFLSANETIKQLKHQKINADYVITANPVESIAALTVFNSDLSIHTNVKAMKRFIKEFASATITQASKSYKENKVMVNKNDFIAVTNKSIIASESQLTDCFFKTIDILSKKVKKPEFLLAYYGKDITEQDAKKMQALVEKKYKLFCEFSPGEQKVFSYIIGIQ", "aa_hexdigest": "941082cccee5a48c9a779484d694c9f6", "start_type": "GTG", "rbs_motif": null, "genes": [ "fakA" ], "truncated": "3-prime", "pseudogene": { "start": 466410, "stop": 467864, "observations": { "mutation": [ 467573 ], "stop": [ 467573 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47609", "query_cov": 1.0, "subject_cov": 0.6894075403949731, "identity": 0.997, "score": 752.0, "evalue": 2.22e-271, "gene_start": 1, "gene_end": 384, "reference_start": 174, "reference_end": 557, "reference_sequence": "MSSVNLSSFIDMLRLGCNNIAKNYEYINQLNVFPVPDGDTGTNMKVTITEAIKKLENEKSHIKSFSELGKNFTRDLLLFSRGNSGVIFSQIMKGFFSNIIVNKTSNNSELSIEDVANAFIVAQEVAYKNVSKPVEGTMLTVARLISNEFKSQKNRPKTLEKLFEQAVKVAWQAVKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSYYGGESNLDDNTLSTIEIKFNKFKEQHANEDEFGYCTEYVLRLGLKINQTVEKQKFHQKKFESKVNRIANSVVIASDKDNGFVKVHAHTLKPHLLLEMGLNYGEFEFVKIDNMNLQVNNKNNSPTKRVLKPAIVATVPTEAFAERIREDHDINAILCTDDTGAPSVFSLLEAVKLTNSSNVIFLLHDKNYFLSANETIKQLKHQKINADYVITANPVESIAALTVFNSDLSIHTNVKAMKRFIKEFASATITQASKSYKENKVMVNKNDFIAVTNKSIIASESQLTDCFFKTIDILSKKVKKPEFLLAYYGKDITEQDAKKMQALVEKKYKLFCEFSPGEQKVFSYIIGIQ" }, "paralog": false, "identity": 0.9979338842975206, "score": 941.0, "evalue": 0.0, "description": "Internal stop codon at 467573. Nonsense mutation around 467573.", "psc": { "uniref90_id": "UniRef90_P47609", "gene": "FakA", "product": "Uncharacterized protein MG369", "uniref50_id": "UniRef50_P75231", "cog_id": "COG1461", "cog_category": "I", "go_ids": [ "GO:0004371", "GO:0006071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47609", "UniRef:UniRef50_P75231", "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071" ] }, "pscc": { "uniref50_id": "UniRef50_P75231", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75231" ], "product": "Uncharacterized protein MG369 homolog" } }, "id": "KKNAAMEMLJ_837", "locus": "GCA000027325_00836" }, { "type": "cds", "sequence": "contig_1", "start": 467571, "stop": 468083, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATCAGTAAATTTATCTTCTTTTATCGATATGTTACGCCTTGGCTGTAATAACATCGCTAAAAATTATGAATACATAAACCAGTTGAATGTTTTTCCTGTTCCTGATGGTGACACTGGAACAAACATGAAAGTAACAATAACAGAAGCCATCAAGAAACTAGAAAATGAAAAAAGTCATATAAAAAGCTTTAGTGAACTTGGTAAAAACTTTACTAGAGATTTACTTTTGTTTTCACGTGGCAATTCAGGGGTTATCTTTTCACAAATAATGAAAGGATTTTTCAGTAACATAATCGTAAATAAAACAAGCAATAATTCAGAGTTGAGTATAGAAGATGTTGCAAATGCATTCATTGTTGCTCAAGAAGTGGCTTATAAAAACGTATCAAAACCAGTAGAAGGAACTATGCTAACAGTTGCTCGTTTAATAAGCAATGAGTTTAAGAGTCAAAAAAATCGTCCTAAAACACTTGAAAAACTTTTTGAACAAGCTGTTAAAGTAGCTTGA", "aa": "MSSVNLSSFIDMLRLGCNNIAKNYEYINQLNVFPVPDGDTGTNMKVTITEAIKKLENEKSHIKSFSELGKNFTRDLLLFSRGNSGVIFSQIMKGFFSNIIVNKTSNNSELSIEDVANAFIVAQEVAYKNVSKPVEGTMLTVARLISNEFKSQKNRPKTLEKLFEQAVKVA", "aa_hexdigest": "3d8ff73212fe5eef2bbff17ed045ab71", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18991.6027, "isoelectric_point": 9.16187610626221 }, "id": "KKNAAMEMLJ_838", "locus": "GCA000027325_00837" }, { "type": "cds", "sequence": "contig_1", "start": 468152, "stop": 468421, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTAATAACAATTAGATTAATTAGTGGTAGGACTCACCAAATTCGTGCGTGTTTAAATTTATTTTCAAATCAACTAGTTGGTGATAAAAAATACAGCTTGATTCAATTTAAAAATCGTAATAATAAATATAAGCATCAAGCACTTCACGCTTATGAATTAGTTTTTCCTAAACTAGAAACGAGCAAGTTTCCCATTTTAACAAATTATTCATTAATGCAATTCAAGAGTAAAATAGTACCTTGGTTTGAATATTTAATTCAATAG", "aa": "MSLITIRLISGRTHQIRACLNLFSNQLVGDKKYSLIQFKNRNNKYKHQALHAYELVFPKLETSKFPILTNYSLMQFKSKIVPWFEYLIQ", "aa_hexdigest": "e85fdb441f3f920480a09b477d5db21e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10607.384099999994, "isoelectric_point": 10.00628299713135 }, "id": "KKNAAMEMLJ_839", "locus": "GCA000027325_00838" }, { "type": "cds", "sequence": "contig_1", "start": 468530, "stop": 469123, "strand": "-", "frame": 2, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG370", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0009982", "GO:0140098", "SO:0001217", "UniRef:UniRef50_P47610", "UniRef:UniRef90_P47610" ], "nt": "ATGAGAATAGCTAATAAATTTTTAGTTCCTAAAGAAAGTGAAAACCAACGAATTGACCAATTTTGTTTAAAAATCCTTCCCTTAATTAAAAGAAGTGACTTTTTTAAGTATTTACGCTTAGGAAAGGTTTTGTTAAATAAAACAAAACCACAGGTCAACACAAGATTAAAAACTAAAGATGAGATAGCTTTTCTTTTTGATATAAATCCCTATTTACAAACTAATAATGACTATTTATCTCTAGATTTGGTAAAAGATAAGCTAAAAATTATATTTGAGGATGAAAATATTATTGTAGTTGACAAACCAACTGGAATAGTTTGCCAACCTGATAAAAAACATAGCATTATTAATTTATCAAATATGCTTTTAAAACACTGTGGATATAGACAATTTGACAGTAATAAACTTAACTTTTATCCCCAGTTTGCACATCGTATTGATCGTAATACTTCTGGAATTGTTATTGGTGCTAAAACAAACAAAGCTTTAAAAGAATTGAACAAAGTTTTTAAAAATAACCATTTAACCAAAAGATATAAAGGCTTAGTTTTTGGTCAATTTAACCATCTTGGCTTACAAACTGCTTATTGA", "aa": "MRIANKFLVPKESENQRIDQFCLKILPLIKRSDFFKYLRLGKVLLNKTKPQVNTRLKTKDEIAFLFDINPYLQTNNDYLSLDLVKDKLKIIFEDENIIVVDKPTGIVCQPDKKHSIINLSNMLLKHCGYRQFDSNKLNFYPQFAHRIDRNTSGIVIGAKTNKALKELNKVFKNNHLTKRYKGLVFGQFNHLGLQTAY", "aa_hexdigest": "cf3bab7d7220f2de23be0691d9409eea", "start_type": "ATG", "rbs_motif": null, "genes": [ "rluA" ], "truncated": "5-prime", "pseudogene": { "start": 468227, "stop": 469123, "observations": { "mutation": [ 468493, 468532 ], "stop": [ 468493, 468532 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47610", "query_cov": 1.0, "subject_cov": 0.6099071207430341, "identity": 1.0, "score": 396.0, "evalue": 8.17e-138, "gene_start": 1, "gene_end": 197, "reference_start": 1, "reference_end": 197, "reference_sequence": "MRIANKFLVPKESENQRIDQFCLKILPLIKRSDFFKYLRLGKVLLNKTKPQVNTRLKTKDEIAFLFDINPYLQTNNDYLSLDLVKDKLKIIFEDENIIVVDKPTGIVCQPDKKHSIINLSNMLLKHCGYRQFDSNKLNFYPQFAHRIDRNTSGIVIGAKTNKALKELNKVFKNNHLTKRYKGLVFGQFNHLGLQTAYWKKDNNNGIVTVKWKPFPEAKKISTFFENSSYIAQKDMSLITIRLISGRTHQIRACLNLFSNQLVGDKKYSLIQFKNRNNKYKHQALHAYELVFPKLETSKFPILTNYSLMQFKSKIVPWFEYLIQ" }, "paralog": false, "identity": 0.9932885906040269, "score": 593.0, "evalue": 9.13e-218, "description": "Internal stop codon at 468493, 468532. Nonsense mutation around 468493, 468532.", "psc": { "uniref90_id": "UniRef90_P47610", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG370", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47610", "cog_id": "COG0564", "cog_category": "J", "go_ids": [ "GO:0140098", "GO:0009982", "GO:0003723", "GO:0000455" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47610", "EC:5.4.99.-", "UniRef:UniRef50_P47610", "COG:COG0564", "COG:J", "GO:0140098", "GO:0009982", "GO:0003723", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47610", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47610" ], "product": "Uncharacterized RNA pseudouridine synthase MG370" } }, "id": "KKNAAMEMLJ_840", "locus": "GCA000027325_00839" }, { "type": "cds", "sequence": "contig_1", "start": 469110, "stop": 469229, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAATGGTGGTGGACATAAATATGCAGCTGCTTTTGTTTTGGATGAAAAAAATCAATTTATGAAATTAGTACAGATAATGGATGATTTTCTTGCCAAACAAAATGAGAATAGCTAA", "aa": "MFNGGGHKYAAAFVLDEKNQFMKLVQIMDDFLAKQNENS", "aa_hexdigest": "6349d3033f3deba959e08d9f64c10586", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4451.0236, "isoelectric_point": 5.498172187805174 }, "id": "KKNAAMEMLJ_841", "locus": "GCA000027325_00840" }, { "type": "cds", "sequence": "contig_1", "start": 469278, "stop": 469532, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACTGGTTTTAACCGCAATAAGGTTCATGATGCAGTTTATTTAAAGCCATTACTAGAACATAAGTACTTTAGCTATGTACTTAGCAAAGCCAAAATAACTAAAAATGGGTTAGCTTATGCATTGATTAAAAAAGGAGCATATAAACATTTTGGTGTTGTCTCTCCTTTACCAATGGTTCATGCTTTGAATAACATAAAAGGAGTAAAAATATGGACTACTGTTTACTTTAACGAGTCCATTAAAAAATGA", "aa": "METGFNRNKVHDAVYLKPLLEHKYFSYVLSKAKITKNGLAYALIKKGAYKHFGVVSPLPMVHALNNIKGVKIWTTVYFNESIKK", "aa_hexdigest": "3973dcb71ca265a51be8444ae6032056", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9612.270499999997, "isoelectric_point": 9.953547859191893 }, "id": "KKNAAMEMLJ_842", "locus": "GCA000027325_00841" }, { "type": "cds", "sequence": "contig_1", "start": 469578, "stop": 469676, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGTTATTTAATTTTGCAGATGGGTTACAAATTAAATGATGAGATTGCATCATATCTTTATGCAGGAATAATTACTGATACACAGCGATTCTGA", "aa": "MIGYLILQMGYKLNDEIASYLYAGIITDTQRF", "aa_hexdigest": "59cfb9f83911f8505c9aa190a1ea6976", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3715.294899999999, "isoelectric_point": 4.556577491760254 }, "id": "KKNAAMEMLJ_843", "locus": "GCA000027325_00842" }, { "type": "cds", "sequence": "contig_1", "start": 469707, "stop": 470084, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGATTAGTATCGATCCACAATTCATTAAGAATTTTTCAAAAAAGGTTAAACAGTTTGATAAGTTCAGTTTATTTGTACATGTAAACCCAGACTTTGATGCATTTGGATCTGCTTTTGCTTTCAAAACTTTCCTAAATACTTTTTTCAGTGAAAAAAAAGCTTATGTTATGGGTTCATACAATATTAATGCAGACGGTAGAGAACTTTTTCCTTTTGAGCAAACTGACATAAATGATGATTTTGTAAAAGAATCTTTAGCTATTATTTTTGATACTTCCAACCAAGAAAGAGTTTTAACTCAAAAACATAAGTTGGCAAAAGAGACAGTTAGGATAGATCACCATCCTAGAACTGAAAAATTTGCTGATATGGAATGA", "aa": "MISIDPQFIKNFSKKVKQFDKFSLFVHVNPDFDAFGSAFAFKTFLNTFFSEKKAYVMGSYNINADGRELFPFEQTDINDDFVKESLAIIFDTSNQERVLTQKHKLAKETVRIDHHPRTEKFADME", "aa_hexdigest": "4cfe512bd2c9cd15a27e88cd27053a61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 100, "aa_cov": 0.784, "hmm_cov": 0.75, "evalue": 4.4583955021874725e-11, "score": 38.24043655395508, "start": 24, "stop": 121 } ], "seq_stats": { "molecular_weight": 14628.388799999979, "isoelectric_point": 6.077304649353027 }, "id": "KKNAAMEMLJ_844", "locus": "GCA000027325_00843" }, { "type": "cds", "sequence": "contig_1", "start": 470077, "stop": 471234, "strand": "-", "frame": 3, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "EC:2.8.1.4", "GO:0000049", "GO:0002937", "GO:0004810", "GO:0005524", "GO:0005829", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0140741", "KEGG:K03151", "RefSeq:WP_009885937.1", "SO:0001217", "UniParc:UPI0000136E19", "UniRef:UniRef100_P47612", "UniRef:UniRef50_P47612", "UniRef:UniRef90_P47612" ], "nt": "ATGGAATTGAATAGTGAAGATGTATTAGTTGCGCGTTATGGTGAATTGGTTTTAAAAGGAAAAAACCGTTCTTATTTCACAAAACAATTAAAAATTAACATCAAAAAAGCTTTTAAAAAACTGGAAATTAACAATAGTATTGTTTATGAATTTGATAGAATTGTTGTCTTTGATATTAAAAAAGAACAAAGAGCGATTCTCCAAGAATTATTCAGTTTTTTGCCTGGAATTAGTTTGTTCTTTTTTGCATCACAGATAGTAAGGGAAGAAAACAAACTATTAGATCTTCTTTTTAATTTATTTAAAGATTTTAATTCCTTTAAATTAGAAGTTAAGAGACGTGATAAAAATTTTGCTGAAAATTCAAGTAATTTTAAGAAGTATTTAGCAGTTAAACTATTTGAAAAATACCAATTAAAAGGAGTAATTAATAACCCTGAAATAATAGCTAATATAGAGATTTTGAAGGAGCATTTTCTTGTTTTTACAGAACGTTTTAAAGGTAAAGGTGGATTACCTGTTTATAGTAGTGGTAAAGCTTTAGTCTTACTTTCAGGAGGAATTGATAGCCCAGTTGCAGCTTCACTTGTAATGCAACGTGGTTTTAACATTGATTTTATCACCTTTATTAATGAACCAAATAAGAATCAAAAAACAATTGAAAAAATCACAAGGTTAGCAAATTTAATTTCTTTTAATAAAACGATTTGTAGTGGTAAATTATTAGTTTTTGATTTCACTGCAATTCAAAAAGAGTTAATTCATATTAGTAATGAATCTTATCGTATCGTTTTAATGCGTCGTGTTTTTTATAAAGCTGCTAGTATGTTTAAGTATGATTGTCTAGTTACAGGTGAGGTTTTGGGACAAGTTGCATCCCAAACCATTGAAAATTTAAAAGTTATTCAATCAGCAACACCAGATACATTTATAGTTCGCCCCTTAATTGGCTTTAGTAAAGATAAAATTATTGAATTAGCTAAATTTTTTAATACTTTTGATATTTCTATTGAACAACATTTAGATACTTGTAGTGAGTTTTCTCCCAAAAATCCTACAACGAAAGCTAAATTGATAAATGTTGAAAAGCTTGAAAGTGAGCTTATTTTTTTAAATGATTTAATTGAAAAGGGAGTAAGTGAATTAAGCAATGATTAG", "aa": "MELNSEDVLVARYGELVLKGKNRSYFTKQLKINIKKAFKKLEINNSIVYEFDRIVVFDIKKEQRAILQELFSFLPGISLFFFASQIVREENKLLDLLFNLFKDFNSFKLEVKRRDKNFAENSSNFKKYLAVKLFEKYQLKGVINNPEIIANIEILKEHFLVFTERFKGKGGLPVYSSGKALVLLSGGIDSPVAASLVMQRGFNIDFITFINEPNKNQKTIEKITRLANLISFNKTICSGKLLVFDFTAIQKELIHISNESYRIVLMRRVFYKAASMFKYDCLVTGEVLGQVASQTIENLKVIQSATPDTFIVRPLIGFSKDKIIELAKFFNTFDISIEQHLDTCSEFSPKNPTTKAKLINVEKLESELIFLNDLIEKGVSELSND", "aa_hexdigest": "da46482a56b14043bb349542cd756c8a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000136E19", "ncbi_nrp_id": "WP_009885937.1", "uniref100_id": "UniRef100_P47612", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000136E19", "RefSeq:WP_009885937.1", "UniRef:UniRef100_P47612" ] }, "ips": { "uniref100_id": "UniRef100_P47612", "uniref90_id": "UniRef90_P47612" }, "psc": { "uniref90_id": "UniRef90_P47612", "gene": "thiI", "product": "Probable tRNA sulfurtransferase", "ec_ids": [ "2.8.1.4" ], "uniref50_id": "UniRef50_P47612", "cog_id": "COG0301", "cog_category": "HJ", "kegg_orthology_id": "K03151", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0004810", "GO:0000049", "GO:0140741", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0002937" ] }, "pscc": { "uniref50_id": "UniRef50_P47612", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47612" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [ "thiI" ], "id": "KKNAAMEMLJ_845", "locus": "GCA000027325_00844" }, { "type": "cds", "sequence": "contig_1", "start": 471305, "stop": 471466, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCAATGTAAATAAAGGACTTTTTGCAATTGCCTTTTTGAAACCAGAAGATTATGTCTTACCAGAACAATTTAATGCTAGCAATAGAGAAATTAGGTTAATTCCTATCAAGATAGATCATAAGGGATTTAGTGTTTTAGTTGATAAGGCGAGAATTTGA", "aa": "MRNVNKGLFAIAFLKPEDYVLPEQFNASNREIRLIPIKIDHKGFSVLVDKARI", "aa_hexdigest": "f1bd4561ac382f04c59513d350014452", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6126.139099999999, "isoelectric_point": 9.693288993835449 }, "id": "KKNAAMEMLJ_846", "locus": "GCA000027325_00845" }, { "type": "cds", "sequence": "contig_1", "start": 471533, "stop": 471892, "strand": "-", "frame": 2, "gene": null, "product": "MPN551 family DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227", "UniRef:UniRef90_P47613" ], "nt": "ATGGTCAAAATTTATGAAGATGAATTTGATGAAACACTGGCAAAAGCTGGAAACATAATAGAACCTAAAATACGTGATTATGTCAATTTAAAAACTGGTTTTAATTTTCATTCTTATGATCCTAAAAAAGTTCAATTTGATTTATTTAAGGATGATAGTGTTTTTGGTGGGATACCTGATGGTGAACCTCTTGATGAAAATGGTGAATTAGCTTATCAAAATGATCTTCCTATGTTAGAGATTAAAACAACTTCTTGCGATAGTCTTATTTATAAAAAAATCAATGGAAATCTCAAGATGATTCTTGATGAAAATGGTATGCCTATTGTTAAAAAGAAAGATGGCAAAAAAGATAGTTGA", "aa": "MVKIYEDEFDETLAKAGNIIEPKIRDYVNLKTGFNFHSYDPKKVQFDLFKDDSVFGGIPDGEPLDENGELAYQNDLPMLEIKTTSCDSLIYKKINGNLKMILDENGMPIVKKKDGKKDS", "aa_hexdigest": "c7c7b9ad22adf906217200395552bd5b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 471230, "stop": 472066, "observations": { "mutation": [ 471247, 471307, 471535, 471901 ], "stop": [ 471247, 471307, 471535, 471901 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47613", "query_cov": 1.0, "subject_cov": 0.4234875444839858, "identity": 1.0, "score": 242.0, "evalue": 5.48e-79, "gene_start": 1, "gene_end": 119, "reference_start": 59, "reference_end": 177, "reference_sequence": "MGFIKQYKTDFDIVDNQIVLSEQYFLRNKALFKKITGTRFGKVLGLSEYESSFKTWANMVKIYEDEFDETLAKAGNIIEPKIRDYVNLKTGFNFHSYDPKKVQFDLFKDDSVFGGIPDGEPLDENGELAYQNDLPMLEIKTTSCDSLIYKKINGNLKMILDENGMPIVKKKDGKKDSWFDSNGKIIISTLYYCQIGLYLYLRNVNKGLFAIAFLKPEDYVLPEQFNASNREIRLIPIKIDHKGFSVLVDKARIWYENYILTGKSPKLTESDIQWLKENGIE" }, "paralog": false, "identity": 0.9856115107913669, "score": 545.0, "evalue": 6.93e-201, "description": "Internal stop codon at 471247, 471307, 471535, 471901. Nonsense mutation around 471247, 471307, 471535, 471901.", "psc": { "uniref90_id": "UniRef90_P47613", "product": "MPN551 family DNA-binding protein", "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47613", "UniRef:UniRef50_P75227" ] }, "pscc": { "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227" ], "product": "Uncharacterized protein MG373 homolog" } }, "id": "KKNAAMEMLJ_847", "locus": "GCA000027325_00846" }, { "type": "cds", "sequence": "contig_1", "start": 471899, "stop": 472066, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTTATAAAGCAATATAAAACTGATTTTGATATAGTTGATAATCAAATTGTTCTATCAGAACAGTATTTTTTAAGAAATAAAGCTCTATTTAAAAAAATAACCGGTACTCGCTTTGGCAAGGTTTTAGGTTTATCTGAATATGAGTCATCATTTAAAACTTGA", "aa": "MGFIKQYKTDFDIVDNQIVLSEQYFLRNKALFKKITGTRFGKVLGLSEYESSFKT", "aa_hexdigest": "7517bb11e32be49a605a3309da56ba93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6444.3674999999985, "isoelectric_point": 9.397701454162597 }, "id": "KKNAAMEMLJ_848", "locus": "GCA000027325_00847" }, { "type": "cds", "sequence": "contig_1", "start": 472067, "stop": 472423, "strand": "-", "frame": 2, "gene": null, "product": "MPN552 family protein", "db_xrefs": [ "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75226", "UniRef:UniRef90_P47614" ], "nt": "ATGGATGTTAGTTCTATTTATGTATTTAGTCCTCAGTATCATTTAGACTTTCAACTTATGGAACATATTTCTAGTGGTCAGCCTAAGTTTCATTACAGCAATTTTTATAACGAAAATTTTTTATTTACTAGCACAAAAAATTCCAAGTTTAGTGCTTTAGAAACATATGTTAAAAGCAAGAATTGCTCTTTAAAAGATGTTCATTATTTAAATGTGACAGAAGTTCCAATTAATAACATTAATCAATTAGCAAGTGTTGTATTTTTAAGCAAAAAACAACCAGATAATGATGAGGAATTTAATAATCCTGAAATATCAATTGTTTTAAAAGGAATTTGTGAACAGTTGATGAAATAA", "aa": "MDVSSIYVFSPQYHLDFQLMEHISSGQPKFHYSNFYNENFLFTSTKNSKFSALETYVKSKNCSLKDVHYLNVTEVPINNINQLASVVFLSKKQPDNDEEFNNPEISIVLKGICEQLMK", "aa_hexdigest": "bc1bb6186708604d079a0f72d40bf52e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 472067, "stop": 472723, "observations": { "mutation": [ 472483, 472675 ], "stop": [ 472483, 472675 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47614", "query_cov": 1.0, "subject_cov": 0.4306569343065693, "identity": 1.0, "score": 236.0, "evalue": 7.99e-77, "gene_start": 1, "gene_end": 118, "reference_start": 157, "reference_end": 274, "reference_sequence": "MDRKIVVLDINTANFFNTTKDLEVWKNFFNFIQANNHQLVFMSSCWQQAVLYLLDLLSLDDVDIIAESGAIIWISKTNEFIYQSFLDSVSIDTIVHHAIITNSGVFAIGKSKISEEPNTTINYFISLEKYKQFKLIWLTEFDQTLKYQNFLKNLSEMDVSSIYVFSPQYHLDFQLMEHISSGQPKFHYSNFYNENFLFTSTKNSKFSALETYVKSKNCSLKDVHYLNVTEVPINNINQLASVVFLSKKQPDNDEEFNNPEISIVLKGICEQLMK" }, "paralog": false, "identity": 0.9908256880733946, "score": 426.0, "evalue": 5.9e-154, "description": "Internal stop codon at 472483, 472675. Nonsense mutation around 472483, 472675.", "psc": { "uniref90_id": "UniRef90_P47614", "product": "MPN552 family protein", "uniref50_id": "UniRef50_P75226", "go_ids": [ "GO:0005524" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47614", "UniRef:UniRef50_P75226", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_P75226", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75226" ], "product": "Uncharacterized protein MG374 homolog" } }, "id": "KKNAAMEMLJ_849", "locus": "GCA000027325_00848" }, { "type": "cds", "sequence": "contig_1", "start": 472817, "stop": 472945, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAAATCACCAGAATTAGCTTTAATAATTTTGTTAAAAGAGTTAGAAGAGATGGATAGAAAAATAGTTGTTTTAGATATTAATACAGCAAATTTTTTTAACACTACAAAGGATTTAGAAGTATGA", "aa": "MKKSPELALIILLKELEEMDRKIVVLDINTANFFNTTKDLEV", "aa_hexdigest": "f87a55e59762405bae8c3c7f2ba4f7fe", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4876.728700000001, "isoelectric_point": 4.8126939773559565 }, "id": "KKNAAMEMLJ_850", "locus": "GCA000027325_00849" }, { "type": "cds", "sequence": "contig_1", "start": 472884, "stop": 473468, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25", "PFAM:PF00587.30" ], "nt": "ATGGAGAATGTTTTAAAAGATTTAAATATCCAATATCAAAAAGAGATAGGAGCTGCTGCTTTTTATGGACCAAAAATTGATTTTCAGTTCAAAACAATCTTTAAAAAAATGATTACTATTGCCACCATTCAACTAGATTTTTTACTACCAGAAAAATTTGATCTAACTTATATAGATAAAAAAAATACACTAAAAAAACCAGTTATTATCCATGTTGGAATTATTGGAACTTATGAAAGGTTTATTGCTGCTTTACTTGAAAAAACAAGTGGTAATTTTCCTTTATGGTTAGCACCTGTTCAAGCCGTAATTATTCCTGTTAATATCCAAAAGCATTTAAAGGCAGCAAAAAAACTTTATAACAAATTGCTAAAAGAAAACATCCGTGTAAATTTAGATGATAATCAAGATCGCTTAGCTAAAAAAGTTAGACAAGCAATCATTGAAAAAATTCCTTTACAACTTATTGTTGGAGATAAAGAAATAGAGAATTTAGAGAAGTTGACATGCCGTGGTTTTAAAGGTGAAAAAATCACCAGAATTAGCTTTAATAATTTTGTTAAAAGAGTTAGAAGAGATGGATAG", "aa": "MENVLKDLNIQYQKEIGAAAFYGPKIDFQFKTIFKKMITIATIQLDFLLPEKFDLTYIDKKNTLKKPVIIHVGIIGTYERFIAALLEKTSGNFPLWLAPVQAVIIPVNIQKHLKAAKKLYNKLLKENIRVNLDDNQDRLAKKVRQAIIEKIPLQLIVGDKEIENLEKLTCRGFKGEKITRISFNNFVKRVRRDG", "aa_hexdigest": "2267b94af11ba60b986562197adba930", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 93, "aa_cov": 0.4690721649484536, "hmm_cov": 0.989247311827957, "evalue": 6.810352249977035e-22, "score": 72.59778594970703, "start": 101, "stop": 191 }, { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 92, "aa_cov": 0.44329896907216493, "hmm_cov": 0.9782608695652174, "evalue": 9.086210219482296e-16, "score": 53.49976348876953, "start": 3, "stop": 88 } ], "seq_stats": { "molecular_weight": 22423.315200000012, "isoelectric_point": 9.805141639709472 }, "id": "KKNAAMEMLJ_851", "locus": "GCA000027325_00850" }, { "type": "cds", "sequence": "contig_1", "start": 473487, "stop": 473963, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "GTGCATCACCAGCAACTATTGTTTGGTTTTAATACTGTTTGTTCTCCTGTATTAGCTAACATAGAGCTTTTTAAAATTAGCGGCCACTATCAGCACTATAAGGAAGATATGTTTCCTGCTATTAAACTTGATAGTCAAGCAATGATGCTTCGTCCTATGACATGTCCTCATCACTGTCTGATTTTCAAACAAAAACGATATTCATATAAAAAAATGCCACAGCGCTTTTCAGAAGATTCTATTTTGCATCGTTTTGAAGCCTCTGGAGGATTAATAGGATTAGAAAGAGTGAGGTGCATGACTTTACTTGATAATCACATTTTTTGTCGTGCAGATCAAATTAAAAGTGAGATTAAAAACGCATTTAATTTAATTCAAAAAGTTAATAAAAAATTTGGATTTATATTTGATAGGATAGATCTTTCTCTACATGATCCTAAAAATCAATCAAAATTTATTGATAATCCTGGTTTATGA", "aa": "MHHQQLLFGFNTVCSPVLANIELFKISGHYQHYKEDMFPAIKLDSQAMMLRPMTCPHHCLIFKQKRYSYKKMPQRFSEDSILHRFEASGGLIGLERVRCMTLLDNHIFCRADQIKSEIKNAFNLIQKVNKKFGFIFDRIDLSLHDPKNQSKFIDNPGL", "aa_hexdigest": "01c2181845851cad57c667f04accbeb6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 101, "aa_cov": 0.6329113924050633, "hmm_cov": 0.9801980198019802, "evalue": 4.9177862942966725e-18, "score": 60.883846282958984, "start": 46, "stop": 145 } ], "seq_stats": { "molecular_weight": 18530.4717, "isoelectric_point": 9.259739112854003 }, "id": "KKNAAMEMLJ_852", "locus": "GCA000027325_00851" }, { "type": "cds", "sequence": "contig_1", "start": 474006, "stop": 474188, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAGATCCTTCACAATTACAACTTCAGAGGATTAATGGCATTTTTGCTCAATCAAAAAAAGAATTAGAACAATTAATAAAAGAAAATGAAGAACGCTTGAAGAAGGATCACAGATCTTTAGGTAAACAATTAGAGTTATTTAGCTTTGACCCACTGATCGGTGCAGGTCTTCCTATTTGA", "aa": "MGDPSQLQLQRINGIFAQSKKELEQLIKENEERLKKDHRSLGKQLELFSFDPLIGAGLPI", "aa_hexdigest": "2d1ceafb525218cb246457be55844758", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6846.820599999997, "isoelectric_point": 6.556002616882325 }, "id": "KKNAAMEMLJ_853", "locus": "GCA000027325_00852" }, { "type": "cds", "sequence": "contig_1", "start": 474261, "stop": 474578, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCAGGAGTTCTTTTATTACAAATATGGTTAAAATGCAAATATGCTAATGTTCAGTTTGGTGAACATGGTTTTAATGGAGATGAATTTTATTTAGATTTTTATATAAATGAGAATTTTTCTACAAAACAGTTTGCAAAAATAGAATCTGATTTAAACTCTCTTTCTAGTAAACTAGAAGGGATTTCTCAAAAATTTGTTTCTTTAGATGAAGCATTAAGTTTTTTTGAAAATGATCAGTTTACAAAAAACTTATTAAAAAAAAGCAACTTAAACAAATTTAAAATCACCTTTTTTGAAAATAAACATTTTTGA", "aa": "MLAGVLLLQIWLKCKYANVQFGEHGFNGDEFYLDFYINENFSTKQFAKIESDLNSLSSKLEGISQKFVSLDEALSFFENDQFTKNLLKKSNLNKFKITFFENKHF", "aa_hexdigest": "b4b0e43b237b64ae68489a9309012765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12294.846999999982, "isoelectric_point": 6.08395481109619 }, "id": "KKNAAMEMLJ_854", "locus": "GCA000027325_00853" }, { "type": "cds", "sequence": "contig_1", "start": 474650, "stop": 474799, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224", "UniRef:UniRef90_P47616" ], "nt": "TTGTTTGGTGAACGAAGTTTTACTGATTTTTTTGTTTTTTATGCAAATGGTCAATTAGCATTTGAACTTGAAGCATATACTCAAAAATTTAAAACAATTAGCATCGAAGGTATTCTGCGCACTTACCTAGAAAAACGCAGTGGAATTTGA", "aa": "MFGERSFTDFFVFYANGQLAFELEAYTQKFKTISIEGILRTYLEKRSGI", "aa_hexdigest": "447289fe1f00fc36f0ecc097a52e7c81", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 474578, "stop": 474892, "observations": { "mutation": [ 474652, 474847 ], "stop": [ 474652, 474847 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47616", "query_cov": 1.0, "subject_cov": 0.47115384615384615, "identity": 0.98, "score": 96.7, "evalue": 5.41e-25, "gene_start": 1, "gene_end": 49, "reference_start": 32, "reference_end": 80, "reference_sequence": "MLNCVFLEGEIESTKWSHKKTGFLVTIKQKRLFGERSFTDFFVFYANGQLAFELEAYTQKFKTISIEGILRTYLEKRSGIWKTTIEVVKIMKPNSKVMIDYQES" }, "paralog": false, "identity": 0.9807692307692307, "score": 199.0, "evalue": 4.59e-68, "description": "Internal stop codon at 474652, 474847. Nonsense mutation around 474652, 474847.", "psc": { "uniref90_id": "UniRef90_P47616", "product": "Uncharacterized protein MG376", "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47616", "UniRef:UniRef50_P75224" ] }, "pscc": { "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224" ], "product": "Uncharacterized protein MG376 homolog" } }, "id": "KKNAAMEMLJ_855", "locus": "GCA000027325_00854" }, { "type": "cds", "sequence": "contig_1", "start": 474898, "stop": 475056, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTATGAAAAAACTAATCAGAATGTTAGAGAATATCTTGATGATAAACAAAAGTTTGAAGGTATTAGAACTGCATTAATAGAAGAAAGAATGGTTTTAGAAACAATTAACCACTTTAAATTTCATTTCAACTTAACAGGTCAATTACCTAGCTAA", "aa": "MYYEKTNQNVREYLDDKQKFEGIRTALIEERMVLETINHFKFHFNLTGQLPS", "aa_hexdigest": "79e6bb9022c550ad92e75000777a3f5d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6337.1151999999975, "isoelectric_point": 6.043030738830566 }, "id": "KKNAAMEMLJ_856", "locus": "GCA000027325_00855" }, { "type": "cds", "sequence": "contig_1", "start": 475099, "stop": 475479, "strand": "-", "frame": 3, "gene": null, "product": "MPN555 family protein chaperone", "db_xrefs": [ "GO:0006457", "GO:0015031", "SO:0001217", "UniRef:UniRef50_P75223", "UniRef:UniRef90_P47617" ], "nt": "ATGGCTACTAATCTTAAATCTATTGCAAAGTTGCAAAAACCTATTCAGTATGATAAGGTAATTGAAGTTGATCGTATTTTTGCAGATCCTGCTTTTATTGAGCAACATCGGCAAAGAATTTTAGCTTCTTTTAAAGATGCTAAGGAAAGTGCTCTTTACCACGAACTAACTCATATTGTTATTAAAGATAATTTGTTTTCAGCAGCAATGAATGAAATTGTTAGTTATTTTGAATTTCAAATTAACCCTGAAGAGTTAAAAAATGTTGTTGAAGGCTTGAAAAGAGATGTTGTTAAAGATGCTGATGAAAAAACAATTCAATCAATAGCTGAAAAAATCATTAAAAAAGCTTTAGTATTTAATTTCTTACAAAAGGAATGA", "aa": "MATNLKSIAKLQKPIQYDKVIEVDRIFADPAFIEQHRQRILASFKDAKESALYHELTHIVIKDNLFSAAMNEIVSYFEFQINPEELKNVVEGLKRDVVKDADEKTIQSIAEKIIKKALVFNFLQKE", "aa_hexdigest": "9f972991ffd1a8e89b288a8765ade07b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 474898, "stop": 475479, "observations": { "mutation": [ 475101 ], "stop": [ 475101 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47617", "query_cov": 1.0, "subject_cov": 0.6528497409326425, "identity": 1.0, "score": 241.0, "evalue": 1.69e-79, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MATNLKSIAKLQKPIQYDKVIEVDRIFADPAFIEQHRQRILASFKDAKESALYHELTHIVIKDNLFSAAMNEIVSYFEFQINPEELKNVVEGLKRDVVKDADEKTIQSIAEKIIKKALVFNFLQKEWKVEVSDDIVKRVISLYYEKTNQNVREYLDDKQKFEGIRTALIEERMVLETINHFKFHFNLTGQLPS" }, "paralog": false, "identity": 0.9948186528497409, "score": 372.0, "evalue": 6.25e-134, "description": "Internal stop codon at 475101. Nonsense mutation around 475101.", "psc": { "uniref90_id": "UniRef90_P47617", "product": "MPN555 family protein chaperone", "uniref50_id": "UniRef50_P75223", "go_ids": [ "GO:0006457", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47617", "UniRef:UniRef50_P75223", "GO:0006457", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75223" ], "product": "Uncharacterized protein MG377 homolog" } }, "id": "KKNAAMEMLJ_857", "locus": "GCA000027325_00856" }, { "type": "cds", "sequence": "contig_1", "start": 475526, "stop": 475966, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGTTATCTCAAAATAATGGCACAATTATCAAAATTGATTTAGATATAGCTAATTTGCAAAACTCTGCTAATCCAGTTTATTATGTTCAATATGCGTTTGCAAGAATGAATAGTATTCTTAGAATTGCAAATTCTGATCAATTAAAAGAAATTACTGATTGCAGTCTTTTGATTAATGAAAAAGAGATATCACTTTTAAATCAACTTGTCTATTATCCTTTTATGTTGCAAAAAGCTATGGAAACAGGCGAATTGCACTTATTAACTAACTTTTTATATGAAACTGCTAGTTTATTTCATTCCTGGTATAAAGTTTGCAAAATTAATGATGATAAAAATTCACTTTTATCAGCACAAAGACTTGCTTTATTGAGATCATTACAATTTATAGTTAAACAAATCCTTGATGTTTTGAAGATTTCAACACCACAACAAATGTAA", "aa": "MLSQNNGTIIKIDLDIANLQNSANPVYYVQYAFARMNSILRIANSDQLKEITDCSLLINEKEISLLNQLVYYPFMLQKAMETGELHLLTNFLYETASLFHSWYKVCKINDDKNSLLSAQRLALLRSLQFIVKQILDVLKISTPQQM", "aa_hexdigest": "87c6248af5377605c2de863a68a2612b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 118, "aa_cov": 0.8082191780821918, "hmm_cov": 0.9915254237288136, "evalue": 1.428788422543505e-32, "score": 107.3184814453125, "start": 29, "stop": 146 } ], "seq_stats": { "molecular_weight": 16834.410199999988, "isoelectric_point": 6.714128684997559 }, "id": "KKNAAMEMLJ_858", "locus": "GCA000027325_00857" }, { "type": "cds", "sequence": "contig_1", "start": 475970, "stop": 476146, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTGTGCATTTGATGCTTTAAAAAATACCACTAAAACTTCTTTAAAAATTTTTGCATTACAACTGGTTACTCTCTATAAAAATAAAGAGCTAGTACGTTTGTCAAAACGTGCTGGAAATGTAATCACAATTGAAACAATGCTTTCAATGATTAGTGAAGATGCTGCTAGATGA", "aa": "MYCAFDALKNTTKTSLKIFALQLVTLYKNKELVRLSKRAGNVITIETMLSMISEDAAR", "aa_hexdigest": "5cf8facb3ea03ab024ad0e3e0b54929d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6554.7204, "isoelectric_point": 9.57898654937744 }, "id": "KKNAAMEMLJ_859", "locus": "GCA000027325_00858" }, { "type": "cds", "sequence": "contig_1", "start": 476411, "stop": 476863, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAAAAACACCTTGCTTTACTAAGAAAAATATAAAGATTTTAATTGAATCTGTATCAGCAAATCCTACCGGAAGGATCCATTTAGGTCATGTGAGAATAGCTTTTTTTGGTGATGTTTTAAACAATTTAGCCAAGCTGTTGGGTTATACAACAGTCTGTGAATATTGGGTAAATGATTATGGACAACAAGCACGAGTTTTTAGCTTTAGTGTTTATCAAAGTTTGCAGTTAAAAAAAAATATTGCTATCCAGCAACATCCTGATGGATATAGTGGAATAGTAATAGATAAAATTGCTAGTGAAATTGAAAATTTTCCAGTTGATAATTTAAATTTTGAAGAGTTTTGTAAAACATCATTCTTAGATCATTTTTTAGTTAATTGCACCCAAAAAGTTTTGTCTTTAATTAAAAGTGATTTGAATAAAATCCATGTTTTTATTGATAGTTGA", "aa": "MIKTPCFTKKNIKILIESVSANPTGRIHLGHVRIAFFGDVLNNLAKLLGYTTVCEYWVNDYGQQARVFSFSVYQSLQLKKNIAIQQHPDGYSGIVIDKIASEIENFPVDNLNFEEFCKTSFLDHFLVNCTQKVLSLIKSDLNKIHVFIDS", "aa_hexdigest": "7816ca1076a1f28b8e3604d69c9ac6ab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17073.515300000006, "isoelectric_point": 7.690679359436037 }, "id": "KKNAAMEMLJ_860", "locus": "GCA000027325_00859" }, { "type": "cds", "sequence": "contig_1", "start": 476960, "stop": 477139, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTATCATCAATGATTTAAAAGAATGCATTAGCGCTTTAAAGCTTAAATTTGATGACCAAAAGGAACTTGTTAAACTAGTTAAAAATAATAGTTTTAATGGTTTTTCTTCAACTATTATTTTCCAACTAAAAAGTGAAAATCATAAAAAAATTGCAGATAGTATTGTTGAGTGA", "aa": "MFFIINDLKECISALKLKFDDQKELVKLVKNNSFNGFSSTIIFQLKSENHKKIADSIVE", "aa_hexdigest": "ac98275191574655d733bb35ae25a931", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6820.880599999999, "isoelectric_point": 7.868640327453613 }, "id": "KKNAAMEMLJ_861", "locus": "GCA000027325_00860" }, { "type": "cds", "sequence": "contig_1", "start": 477168, "stop": 478472, "strand": "+", "frame": 3, "gene": "mnmG", "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IKD5" ], "nt": "ATGTCTTTTATCATAACAGTAATAGGTGCTGGGCATGCTGGATTGGAAGCCGCTTTCATTGTAAGCAAATTCAACATCAAAGTAAACCTTTTAGTTCTTGATATAAATCATTTAGGTTCTTGTCCATGTAATCCTTCAATTGGTGGACCTGCTAAGGGAATTGTTACTAGGGAAATTGATGTTTTAGGAGGTATGCAAGCAATTGCTGCTGATAACAATGCCTTACAATATAAATTACTAAATAGTTCAAAAGGACCTGCTGTGCAAGCTATCAGAGCACAAATTGACAAAATAGGTTATAAAAACTGGTTTCAAAGTCAAGTTAAATTAAATAAAAACATTAATCTAATTCAATCTGAAGCAATCAATTTAATTGTTAGAAATGAAAAAATAAAAGGCGTTATTTTAAAAGACGGAAGTGAACTTTTAAGTGATGCGGTTATTATCACTACCGGAACGTACCTAAGATCAAAAACATACTGTGGTAATACAGTTAAAAATCAAGGACCTGATCAATCTAAAAATAGTGAAAAATTAAGCACAAACTTAATTAACAGAGGTTTTAAAACAATTCGTTTAAAAACAGGAACTCCGCCAAGAATTTTAAAAACTTCACTTGACTATAATCAAATGGAATTAGAAATTAATAATAATCAAAACCTTGCTTTTAGTACTACAAATAAAAATTTCTTACCACTTGAAAAACAAATACCTTGTTACTTAGTTCATACCAATCAAAAAATTCACGATCTAATCCTTAAAAACTTAAAAAAATCTGCAATGTTTAATGGTAGTATTTCAGCACAAGGACCACTTTATTGTCCAAGCATTGAAGACAAAGTTTTTAAGTTCTCTCAAAAACCTCGTCACCAAATTTTTGTAGAACCTGAATCATTGAGTCTAGATACTATTTATTTAGCAGGATTATCAACTTCTTTTACACCAGAAATTCAAAAAGAAATCATCCAGCTTTTACCTGGTTTTCAAAATGCAGAAATTAAAAAGTTTGGTTACGCTATTGAATATGATGCTTTTCTATCTAATCAACTAAAACCAACACTTGAAACGAAGTTAATAGAAAACTTGTATTTTGCTGGACAAATTAATGGCACTAGCGGTTATGAAGAAGCTGCTGGTCAAGGTTTGATGGCAGGAATTAATGCTGCTTTAAAATTATTAAAAAAACCACCATTTATTTTGCAACGTAATGAGGCTTATATTGGGGTTATGATTAATGATTTAGTTACTAAAACAATCAGTGATCCATACCGTTTGTTAACATCCAGAGCAGAATATAGACTATGA", "aa": "MSFIITVIGAGHAGLEAAFIVSKFNIKVNLLVLDINHLGSCPCNPSIGGPAKGIVTREIDVLGGMQAIAADNNALQYKLLNSSKGPAVQAIRAQIDKIGYKNWFQSQVKLNKNINLIQSEAINLIVRNEKIKGVILKDGSELLSDAVIITTGTYLRSKTYCGNTVKNQGPDQSKNSEKLSTNLINRGFKTIRLKTGTPPRILKTSLDYNQMELEINNNQNLAFSTTNKNFLPLEKQIPCYLVHTNQKIHDLILKNLKKSAMFNGSISAQGPLYCPSIEDKVFKFSQKPRHQIFVEPESLSLDTIYLAGLSTSFTPEIQKEIIQLLPGFQNAEIKKFGYAIEYDAFLSNQLKPTLETKLIENLYFAGQINGTSGYEEAAGQGLMAGINAALKLLKKPPFILQRNEAYIGVMINDLVTKTISDPYRLLTSRAEYRL", "aa_hexdigest": "c2091ad575b32d3d0cf2e229f8e9ec2d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D362B2", "query_cov": 0.9423963133640553, "subject_cov": 0.9975609756097561, "identity": 0.531, "score": 435.0, "evalue": 9.68e-148, "valid": false, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_A0A654IKD5", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A654IKD5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IKD5" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" }, "genes": [ "mnmG" ], "id": "KKNAAMEMLJ_862", "locus": "GCA000027325_00861" }, { "type": "cds", "sequence": "contig_1", "start": 478473, "stop": 479006, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21680.2", "PFAM:PF13932.14" ], "nt": "TTGAGAAATGACAATGTTCAAGAACGGCTCATTAAAAAAAGCTTTGAACTTGGTTTAACAGATAAAAAAACATATGAATTGTTCCTTAAAAAGGAAAAGAAAAAACAGGAATTAATTTCATTTTTAAAAAACACTCAAGTAGGCAAGGTTAAAGCATTGAAATTCACTAATAAAAATACCGCTCAATCACTTTATGACTTCAACAAACGAAGTGAAATAAATTTAGATAAATTGATCAAAGATCTTCCTGAAAAATACCAATTAGATTCAGAAACACTTAAACAAATTGAAATTGAAATTAAATATGAGGGTTACATAAAGAAAAATGAAAAGTATTTTAAGGGTTTAGATAAATTAAGCAAAATTAAAATTCCTCATACTTTTGATTACCATAAGGTTAAGAATTTAGCTAGTGAAGCTATTTTTAAACTATCTAACTTTAAGCCTAGTAATTTAGCAATTGCAAGTCAAATAGCTGGAGTGAACTTTAATGACATTATAGCCATAAAACATTTTTTAAAAACTTATGAATAA", "aa": "MRNDNVQERLIKKSFELGLTDKKTYELFLKKEKKKQELISFLKNTQVGKVKALKFTNKNTAQSLYDFNKRSEINLDKLIKDLPEKYQLDSETLKQIEIEIKYEGYIKKNEKYFKGLDKLSKIKIPHTFDYHKVKNLASEAIFKLSNFKPSNLAIASQIAGVNFNDIIAIKHFLKTYE", "aa_hexdigest": "9d59526094681e52fd896cf9b3f67b0f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21680.2", "name": "GIDA_C_1st", "length": 94, "aa_cov": 0.4915254237288136, "hmm_cov": 1.0, "evalue": 6.075968604936116e-19, "score": 63.734519958496094, "start": 23, "stop": 109 }, { "id": "PF13932.14", "name": "SAM_GIDA_C", "length": 53, "aa_cov": 0.2994350282485876, "hmm_cov": 1.0, "evalue": 2.5866056035045633e-16, "score": 54.344112396240234, "start": 117, "stop": 169 } ], "seq_stats": { "molecular_weight": 20800.948500000013, "isoelectric_point": 9.61631374359131 }, "id": "KKNAAMEMLJ_863", "locus": "GCA000027325_00862" }, { "type": "cds", "sequence": "contig_1", "start": 478999, "stop": 479577, "strand": "+", "frame": 1, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "COG:COG0357", "COG:J", "EC:2.1.1.-", "EC:2.1.1.170", "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043", "KEGG:K03501", "RefSeq:WP_009885942.1", "SO:0001217", "UniParc:UPI000012B473", "UniRef:UniRef100_P47620", "UniRef:UniRef50_P75220", "UniRef:UniRef90_P47620" ], "nt": "ATGAATAATGCTAATTTTGAAAAATATGTTGATTTAGTTTTTGAAGCAAACAAAAATTTCAACTTAACAGGATTTAAAACAAAAGAAGCTATTTATCAGAATTTAGTTATAGAAATATTGACATTATTTAAAGGATATGAAAAATTTTTTATTGACAAAACTGTAGCAGACTTGGGAAGTGGAAATGGTTCGCCTGGGATAATATTAAAACTGTTATTTCAAAAAATAAAAAAGTTAGTTTTAATTGATAGTAAACACAAAAAAATTAGCTTTTTAAATAAATTAACTAAGCAACTAAATCTGGAGAAAACTGTTGCAATTTGTGAACGAATTGAAGTACATAAAAATCACTATGATGTTATCTGTTCTCGTGGTCTAAGTACGATTATTAAAGTTAATGATTTAGCATTTTCCTTGCTTAACTCAAAAGGTATTATTTTTCATATAAAACAAAGCTTAGACCAATACATTGAATTTGAAAAATCAAATCAGAAGAATCAATTTAACTTGTTATTTATAAAGCACTTTACTAGTCAGAATAAAAAACTAATTTTGATAGCTTTACAAAAAAATGATTAA", "aa": "MNNANFEKYVDLVFEANKNFNLTGFKTKEAIYQNLVIEILTLFKGYEKFFIDKTVADLGSGNGSPGIILKLLFQKIKKLVLIDSKHKKISFLNKLTKQLNLEKTVAICERIEVHKNHYDVICSRGLSTIIKVNDLAFSLLNSKGIIFHIKQSLDQYIEFEKSNQKNQFNLLFIKHFTSQNKKLILIALQKND", "aa_hexdigest": "a4edbded6dab27c0c17cc18c7eaea59b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B473", "ncbi_nrp_id": "WP_009885942.1", "uniref100_id": "UniRef100_P47620", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B473", "RefSeq:WP_009885942.1", "UniRef:UniRef100_P47620" ] }, "ips": { "uniref100_id": "UniRef100_P47620", "uniref90_id": "UniRef90_P47620" }, "psc": { "uniref90_id": "UniRef90_P47620", "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.-", "2.1.1.170" ], "uniref50_id": "UniRef50_P75220", "cog_id": "COG0357", "cog_category": "J", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043" ] }, "pscc": { "uniref50_id": "UniRef50_P75220", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75220" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "KKNAAMEMLJ_864", "locus": "GCA000027325_00863" }, { "type": "cds", "sequence": "contig_1", "start": 479882, "stop": 480226, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG381", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75219", "UniRef:UniRef90_P47621" ], "nt": "ATGTTGATTGATGTTCAACATCATAAAAAGTTACAAAAATGTTTATTAAGAGAAATTCATCTTTTAGTTTGTTTAATTAATAGCAATCAATTTAGTGATGAATTAATGCAAATTATTGAGTGGTATCAGTATTTAAAAAAACACTCTAGTAAACTTTTTGTTATTACTGCAAGTAGTGATAAAAAACCAGTAATAGAGCCAACTATAAATGAATATAAAGCTATTTTTGGAGAGTATTTATCTTCATTTCACCTAGATCTAAAAAATAATCAAAGTAATGATTTATTCCAGAAATTACTTGATCAGATCAAAATAAAAGCTACCTCAAAAACTAGTTTGAGATAG", "aa": "MLIDVQHHKKLQKCLLREIHLLVCLINSNQFSDELMQIIEWYQYLKKHSSKLFVITASSDKKPVIEPTINEYKAIFGEYLSSFHLDLKNNQSNDLFQKLLDQIKIKATSKTSLR", "aa_hexdigest": "deb57aafa407fa60704d744034546606", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 479582, "stop": 480226, "observations": { "mutation": [ 479585 ], "stop": [ 479585 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47621", "query_cov": 1.0, "subject_cov": 0.5229357798165137, "identity": 1.0, "score": 224.0, "evalue": 8.23e-73, "gene_start": 1, "gene_end": 114, "reference_start": 105, "reference_end": 218, "reference_sequence": "MIKLGWASFSNTEFAQDIFIKFANQFYKQDDAGTILFELKKTLGVNQLDEIEKNKKVIIVNQFQNSLGKFLLFNKENTKRINSLANEHISSFLKKIFRLSGFKDMLIDVQHHKKLQKCLLREIHLLVCLINSNQFSDELMQIIEWYQYLKKHSSKLFVITASSDKKPVIEPTINEYKAIFGEYLSSFHLDLKNNQSNDLFQKLLDQIKIKATSKTSLR" }, "paralog": false, "identity": 0.9953271028037384, "score": 419.0, "evalue": 3.72e-152, "description": "Internal stop codon at 479585. Nonsense mutation around 479585.", "psc": { "uniref90_id": "UniRef90_P47621", "product": "Uncharacterized protein MG381", "uniref50_id": "UniRef50_P75219", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47621", "UniRef:UniRef50_P75219" ] }, "pscc": { "uniref50_id": "UniRef50_P75219", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75219" ], "product": "Uncharacterized protein MG381 homolog" } }, "id": "KKNAAMEMLJ_865", "locus": "GCA000027325_00864" }, { "type": "tRNA", "sequence": "contig_1", "start": 480240, "stop": 480315, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 73.5, "nt": "GCGTCGGTAGCTCAGTGGATAGAGTACACGCCTTCTAAGCGTGTTGTCGTGGGTTCAAATCCCTCCCGATGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 480280, 480282 ], "id": "KKNAAMEMLJ_17", "locus": "GCA000027325_00865" }, { "type": "cds", "sequence": "contig_1", "start": 480691, "stop": 480894, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00485.24" ], "nt": "ATGGATGAAAAAGGGATTTTAGTTGCAATTAGTGGTGGTAGTTGCTCAGGAAAAACTACTGTTGCTGAAATGATTTATCAACTTTTAAGTAAAAAATTAAAAGTTGCGATCATCTGTCAAGATAACTATTACAAGTCCTATAAAAATAAGCCATTATTAAAAAGAAAAACAATAAACTTTGATCATCCTGATGCTTTTGATTGA", "aa": "MDEKGILVAISGGSCSGKTTVAEMIYQLLSKKLKVAIICQDNYYKSYKNKPLLKRKTINFDHPDAFD", "aa_hexdigest": "a0000b9afdf58a61839e54b3f3972c66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00485.24", "name": "PRK", "length": 69, "aa_cov": 0.8955223880597015, "hmm_cov": 1.0, "evalue": 8.855391829382882e-07, "score": 23.982669830322266, "start": 8, "stop": 67 } ], "seq_stats": { "molecular_weight": 7541.7423, "isoelectric_point": 9.171868705749514 }, "id": "KKNAAMEMLJ_866", "locus": "GCA000027325_00866" }, { "type": "cds", "sequence": "contig_1", "start": 481329, "stop": 481484, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCATTACTTATAAGGAACTTGATCAATATTTAAAAGGTGATTTAATACTTAGTTCAGAAAAGCAAAAAATTGTTTTAGATTTGAAAGCAAAAGCAGAGCATAAACATAATTCACCTTTGAAATTTAAACATCTCTATAATTTCCAGAACTAA", "aa": "MGITYKELDQYLKGDLILSSEKQKIVLDLKAKAEHKHNSPLKFKHLYNFQN", "aa_hexdigest": "9fda724ba694131d3d3ba14dab2e308e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6016.9207, "isoelectric_point": 9.302675056457517 }, "id": "KKNAAMEMLJ_867", "locus": "GCA000027325_00867" }, { "type": "cds", "sequence": "contig_1", "start": 481632, "stop": 482075, "strand": "-", "frame": 1, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "COG:COG0171", "COG:H", "EC:6.3.1.5", "GO:0003952", "GO:0004359", "GO:0005524", "GO:0005737", "GO:0008795", "GO:0009435", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47623", "UniRef:UniRef90_P47623" ], "nt": "ATGACTAATTTAATTAAATATTTAAAAGAACTCCAAAACTGGCTGTTTGATTATGTAAAAAAATCTAAAGCTAAAGGTGTTATTTTTGGCTTATCTGGAGGAATTGATTCAGCAGTTGTTGCTGCTATTGCTAAAGAAACTTTTGGTTTTGAAAACCATTTAGCTTTAATAATGCATATTAATAATTCAAAACTTGATTTTCAAGCAACTAGTGAACTTGTTAAAAAAATGCAATTTAATAGTATTAACATTGAACTGGAAGAGAGTTTCAATCTGTTAGTAAAAACCCTTGGAATAGATCCAAAAAAAGATTTTTTAACAGCTGGTAACATTAAAGCACGTTTACGGATGATAACTTTATATGCTTATGCTCAAAAACACAACTTCTTAGTTTTAGGTACTGGTAATTTTGTAGAGTATACACTTGGTTATTTCACAAAATGA", "aa": "MTNLIKYLKELQNWLFDYVKKSKAKGVIFGLSGGIDSAVVAAIAKETFGFENHLALIMHINNSKLDFQATSELVKKMQFNSINIELEESFNLLVKTLGIDPKKDFLTAGNIKARLRMITLYAYAQKHNFLVLGTGNFVEYTLGYFTK", "aa_hexdigest": "b32fde3d78a1939af3058617b114fb9f", "start_type": "ATG", "rbs_motif": null, "genes": [ "nadE" ], "truncated": "5-prime", "pseudogene": { "start": 481329, "stop": 482075, "observations": { "mutation": [ 481634 ], "stop": [ 481634 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47623", "query_cov": 1.0, "subject_cov": 0.592741935483871, "identity": 1.0, "score": 290.0, "evalue": 8.23e-98, "gene_start": 1, "gene_end": 147, "reference_start": 1, "reference_end": 147, "reference_sequence": "MTNLIKYLKELQNWLFDYVKKSKAKGVIFGLSGGIDSAVVAAIAKETFGFENHLALIMHINNSKLDFQATSELVKKMQFNSINIELEESFNLLVKTLGIDPKKDFLTAGNIKARLRMITLYAYAQKHNFLVLGTGNFVEYTLGYFTKWGDGACDIAPLAWLLKEDVYKLAKHFNIPEIVITRAPTASLFEGQTDETEMGITYKELDQYLKGDLILSSEKQKIVLDLKAKAEHKHNSPLKFKHLYNFQN" }, "paralog": false, "identity": 0.9959677419354839, "score": 491.0, "evalue": 1.32e-179, "description": "Internal stop codon at 481634. Nonsense mutation around 481634.", "psc": { "uniref90_id": "UniRef90_P47623", "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "ec_ids": [ "6.3.1.5" ], "uniref50_id": "UniRef50_P47623", "cog_id": "COG0171", "cog_category": "H", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0004359", "GO:0046872", "GO:0003952", "GO:0008795", "GO:0009435" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47623", "EC:6.3.1.5", "UniRef:UniRef50_P47623", "COG:COG0171", "COG:H", "GO:0005737", "GO:0005524", "GO:0004359", "GO:0046872", "GO:0003952", "GO:0008795", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_P47623", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47623" ], "product": "NH(3)-dependent NAD(+) synthetase" } }, "id": "KKNAAMEMLJ_868", "locus": "GCA000027325_00868" }, { "type": "cds", "sequence": "contig_1", "start": 482251, "stop": 483231, "strand": "-", "frame": 3, "gene": null, "product": "GTPase Obg", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2S3C0" ], "nt": "TTGCAAGCTGATCATAACTGCGATTCACTTTTTTTCTTGAAGAATAAAAAACACCTGTTTGCAGAAGATGGACAAAATGGTAAACCAGATTTAGCACATGGTAAAAATGGTTCAGATCTTTTAATTAAAGTGCCAATAGGAACAACAGTTAAAAACTTAGAAAATAATAGTGTATTGGTGGATTTTGTTCATGATAAACAGAGTTTTATTTTGTGTTTTGGCGGAAAAGGCGGAAAAGGAAATGCTGCTTTTAAAAGCCCAATTATGCGTGCTCCTAATCTATATGAAAATGGTGATAAAGGAGAGATTTTAAACGTTAGTTTGGAAGTTAAATATTTAGCTAATGTAGGAATTGTTGGTTTTCCAAATTCAGGTAAATCAACACTAATCTCCAAACTTTCAAATGCCAAACCTAAAATTGCAAACTATCGCTTTACTACTTTAATACCCGTACTTGGTGTAGTTAAATATCAAAATAATAGTTTAGTTTTTGCTGATATTCCTGGTTTGATTGAAAATGCTAGTGAAGGAAGTGGGTTAGGACATGATTTTTTACGGCATATAGAGAGGTGTGAAATATTAATTCATTTAATTTCACTTGATCCTGTAGATAATGATGATCCTTGCAAAGCATATTTACAAATAATGGATGAACTTTCAAAATATTCTCCCCTTCTAGTTAAAAAAAAGATGCTTGTTGTAGCTAATAAAATTGATGTTAATGAAGGTGAGAAACGGTTTAAAAAATTAGAAAAATTTCTTCAAAAAAAATCTATTTCTGTATTAAAAATTTCTGCTTTAAAGAAAGAATTAGGCAATTTACTTGATAGAGTTTTTGAACTTTACAATAAAACAATTTCCCAATTTGGAGCTAATAAGTTTAGTTTGCCAATGGAACTTGAGAAGCATTATGTTTTTCAAAATACCAATGAAAATAATAACGACCCTTTAAATATTGAAAAAGATAGTTTAAACAGATGA", "aa": "MQADHNCDSLFFLKNKKHLFAEDGQNGKPDLAHGKNGSDLLIKVPIGTTVKNLENNSVLVDFVHDKQSFILCFGGKGGKGNAAFKSPIMRAPNLYENGDKGEILNVSLEVKYLANVGIVGFPNSGKSTLISKLSNAKPKIANYRFTTLIPVLGVVKYQNNSLVFADIPGLIENASEGSGLGHDFLRHIERCEILIHLISLDPVDNDDPCKAYLQIMDELSKYSPLLVKKKMLVVANKIDVNEGEKRFKKLEKFLQKKSISVLKISALKKELGNLLDRVFELYNKTISQFGANKFSLPMELEKHYVFQNTNENNNDPLNIEKDSLNR", "aa_hexdigest": "7b8985391b5fb4e4d156ae57c3e0421d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432RYP9", "query_cov": 0.8773006134969326, "subject_cov": 0.8461538461538461, "identity": 0.5, "score": 274.0, "evalue": 3.27e-87, "valid": false, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q2S3C0", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q2S3C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2S3C0" ], "product": "GTPase Obg" }, "genes": [], "id": "KKNAAMEMLJ_869", "locus": "GCA000027325_00869" }, { "type": "cds", "sequence": "contig_1", "start": 483369, "stop": 483545, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAACTTTTTTTTAATCAGATCAAAATATGCAATAATAATTGCTGAAAATCTCTTAAAGACAGGTAAATCTAAAACTAAAAATCGACAAAAGTTGCAACAAATTCAAAGTGATCTAGAAAGAAAAAAATCTGCAGAAATCGTTTTTAAAATAAAGAAAAAGAAACCAAAATAA", "aa": "MTNFFLIRSKYAIIIAENLLKTGKSKTKNRQKLQQIQSDLERKKSAEIVFKIKKKKPK", "aa_hexdigest": "407bc0392a647678fedd91e7da762425", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6850.172500000002, "isoelectric_point": 10.582372474670407 }, "id": "KKNAAMEMLJ_870", "locus": "GCA000027325_00870" }, { "type": "cds", "sequence": "contig_1", "start": 484070, "stop": 484516, "strand": "-", "frame": 2, "gene": "ugpQ", "product": "putative protein MG385", "db_xrefs": [ "COG:COG0584", "COG:I", "GO:0006629", "GO:0008081", "SO:0001217", "UniRef:UniRef50_P47625", "UniRef:UniRef90_P47625" ], "nt": "TTGAATTTAAACCAAATTAATTTAGTTGATCTTAAAAAGGTTAACCTTGCAAGTGAATTTAAGCTTAAGGTAACTTCACAACAAATTCAAACTTTAAAGGAAGTTTTAACGCAATTTATTCAGCCCTTTAAATATTTGCTGATTCATATTAAAGATGATAAAGAAAATAACAATTCATTATTAGAACAGTTGAATTTGCTTTGCAAAGATTTTGTCTTAGCTAAAGAAAAAATGATTTTGTTAAGTAGTAATTTTCATATCATTAAGTTAATAAATGAGACTTTCAAAGGCTTTAAAACCGGTTTTGTAATTGCAAACAAAAAAGCTTTATTAGTAGCTTCTAGAGACGAATTAATGAGATATTGCCGTTTTTTAGTGCCAAACGAGAGTTTTTATCATAAGAACTGCAAAGAAATTCAAAACCTGGGATTACCAGTTATTCTTTGA", "aa": "MNLNQINLVDLKKVNLASEFKLKVTSQQIQTLKEVLTQFIQPFKYLLIHIKDDKENNNSLLEQLNLLCKDFVLAKEKMILLSSNFHIIKLINETFKGFKTGFVIANKKALLVASRDELMRYCRFLVPNESFYHKNCKEIQNLGLPVIL", "aa_hexdigest": "5e07803e801b973a2b11c61d93e0224b", "start_type": "TTG", "rbs_motif": null, "genes": [ "ugpQ" ], "truncated": "both", "pseudogene": { "start": 483989, "stop": 484699, "observations": { "mutation": [ 484072, 484594 ], "stop": [ 484072, 484594 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47625", "query_cov": 1.0, "subject_cov": 0.6271186440677966, "identity": 0.993, "score": 287.0, "evalue": 9.43e-97, "gene_start": 1, "gene_end": 148, "reference_start": 62, "reference_end": 209, "reference_sequence": "MRKQFLIAYRGYSSIAPENTKLAFQAAQVFDFDGIWIDIQLTKDKQIVVTHKENFKVSNKNLNLNQINLVDLKKVNLASEFKLKVTSQQIQTLKEVLTQFIQPFKYLLIHIKDDKENNNSLLEQLNLLCKDFVLAKEKMILLSSNFHIIKLINETFKGFKTGFVIANKKALLVASRDELMRYCRFLVPNESFYHKNCKEIQNLGLPVILWVIKGLLRYQFYENDRFVKFQITAQIY" }, "paralog": false, "identity": 0.9915254237288136, "score": 457.0, "evalue": 3.16e-166, "description": "Internal stop codon at 484072, 484594. Nonsense mutation around 484072, 484594.", "psc": { "uniref90_id": "UniRef90_P47625", "gene": "UgpQ", "product": "Uncharacterized protein MG385", "uniref50_id": "UniRef50_P47625", "cog_id": "COG0584", "cog_category": "I", "go_ids": [ "GO:0008081", "GO:0006629" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47625", "UniRef:UniRef50_P47625", "COG:COG0584", "COG:I", "GO:0008081", "GO:0006629" ] }, "pscc": { "uniref50_id": "UniRef50_P47625", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47625" ], "product": "Uncharacterized protein MG385" } }, "id": "KKNAAMEMLJ_871", "locus": "GCA000027325_00871" }, { "type": "cds", "sequence": "contig_1", "start": 484592, "stop": 484699, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGAAAACAGTTTTTAATAGCTTATCGCGGTTATAGTTCTATTGCACCAGAAAACACCAAATTAGCATTTCAAGCAGCCCAAGTTTTTGATTTTGATGGTATTTGA", "aa": "MRKQFLIAYRGYSSIAPENTKLAFQAAQVFDFDGI", "aa_hexdigest": "096cf6b28b10c2b2b4fc0ba311cdc327", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3997.532699999999, "isoelectric_point": 8.188854789733888 }, "id": "KKNAAMEMLJ_872", "locus": "GCA000027325_00872" }, { "type": "cds", "sequence": "contig_1", "start": 484947, "stop": 485216, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCATTATGAACCTGATGGAGAATGAGTTTGAGCAGGATTCTTTGGTGATGATCAAAAATGAAATAAGGATGCAACTGTTAAATGAGCTAGAGAAAGAGATTATTTACCACTTATTGGTGATGAAGTTTATGGTAGGTACAACAATAAAGGTGAGTGGATTTGATATGGTTTCTATGATGAATCTGGAGACTGAGTTCTTGTTGATGAACAATGAAAGAATCGTCAACCACGAATTAACGAGGCACCTAAGTTTTGAGAGAAATTAA", "aa": "MVIMNLMENEFEQDSLVMIKNEIRMQLLNELEKEIIYHLLVMKFMVGTTIKVSGFDMVSMMNLETEFLLMNNERIVNHELTRHLSFERN", "aa_hexdigest": "8707d5869708f37541a4b5a16d0e55d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10682.52679999999, "isoelectric_point": 4.9306348800659165 }, "id": "KKNAAMEMLJ_873", "locus": "GCA000027325_00873" }, { "type": "cds", "sequence": "contig_1", "start": 485242, "stop": 487647, "strand": "-", "frame": 3, "gene": null, "product": "Protein P200", "db_xrefs": [ "GO:0020035", "SO:0001217", "UniRef:UniRef50_Q49429", "UniRef:UniRef90_Q49429" ], "nt": "TTGGAATATAAAGTAGATTTCCTTGAAACTGAACCAAAATCATTATTTGATGAAAAAACAACAATAGTTGTAGAAAGTGAACCACCTTTCATTCAACCTGACCTATCACTTGAATTAGATTCTGTAAATGATGTTGATAAATCATTAGAAACTAAAACTACATCTGTTGAATTAAATCATGAAGAAATTGGTAATGAATTTATTAATTTAGATGTTTCTGAAAAAGAAGTTCAAGAACAACCAACAACACAACTAGAAACTGATAGTGAGTTCGTATTACCAACATATCAAATAGTTGAAGACAGTTTCACTGAAAGTGCTGAAACTCCTAATGAATTTTCATCTGAACAAAAAGATACGCTTGAATTCATTAGTCAAACTCAAGAGGTTGAGACTTCTGAATCAAATGTACCCACAGTTGAACAAGAAACAAAATTATTTGAACATCAAGATGAAAATAACTTATTCACTCCTCTACCGCTTGATTTAACTGAAATCATTGAATCAAATGCTTTATTTGATTCAAAACCAGACGAAAAAGAGAGTTCTGATTCAGAACTCCAACCTACTTTCAAAGAAATTAAATTAGATTCAACTGTTGAAGTTCCTCAAGAATCATCACAAGTTGAAGCTACTTTTGATACTGTTCAACCAGAAGCAGTTTTTGATGAAATTAAAACTCAAGAACTACAGCCTGAAGCTACTACTGAAGTTGTTTTTGATGATCATTTTCAACCAGATGTTCAACCTGAACAAACTCCTCAAGAAGCTAAATTTGATTCACCAGTCGAAATTCCCCAAGAATCATCGCAAGCTGAATTTCATGCTGAACAAATCTCTGATGAAATTAAATTAGAAGAAAAAACTGAAGCAGTTTTTGATCATCAACAATTAGAAAATCAGTCTGAAGAAACAGTTGTTACACCTACTGAAGTTACTGCTTTTGAACCAGAAACAATTGAAACCCAATTAGAACCTAGCTCTGAAGATCAACCATCTGAACCTGCATTAGATCAAAATCATCCAGAAATTGTTACTGCTGAAGTTGAACAAATCTTTGATGGAACTAAGTTAGAAGATTTAAAACTAGAGGAAGCTAACTTTGATAATGTTGAAAATAATGAAGTTCAACCTAAAGAAACAGAAGCTGAAATTACTTTTGATGAAACTAAAGAACTTCAACAGGAAACCTCCTCTGAACCATTATCAACAGAAGAATTAAAATCTGAAGCTACTTTTGATAATGTATCTGAAGCTGAATCAGAAGCAGTTTTTGAAAAACCTCAATTAGAAACACAGACTGAAAAAATATTAGAAGAGGAACCTAAATCTGAACCAGTTGATCAATTAATAACTGAAGCTAGCTTTGATACTGTTAAACATGAAGCAGTTTTTGATAAAAATCAAACTCAAACTGAGGGATTGGAAGAACCTCAAGTTTCTAGTGAAGCTGAAGTAGTTGATCAAACAACTACTGATACTGTTGGAGAACCTGAAGCAGTTTTTGATGTTCAACCTGAAAAAACTACTGAAGTTAAGTTTGATGATGTTGAAAATCAACAAAAAGTTATTTCTGAACCTCAAGTAGAACAACAACCTGGAGAAGCAGTTTTTGAACCATCTGCAGAAGCTAAATTTGATTCACCAGTTGAATCGGTTCAAGATTCACAACCTGAACCTGTCCTTGAAGAAGTTCAAACTCAACCAGAAATTCAACCTGTAGAATCTCAACCAGAAGCTACCTTTGATACTGTTCAACCTGAACAAACTCCTCAAGAAGCTAAATTTGATTCACCAGTTGAAACTGTTGAACAACCTGAATTTAGTAGTGAACCAACTCAACAACATGTTGAATCAGAAGCTAGTTTTGATGAACCTAATTATGACTTTGATGAACCTAACTATGATTTTGATCAACCTAGTTATGATTCAGATCTACAACCATCAGAACCTCAATATGATGTAGATGAACCTAATTATGACTTTGATGAACCAAATTATGAAATTGAATCAAAACCATCAGAGCCACAATTTGAACCTCAAGTAGAACAACAACCTGGAGAAGCAGTTTTTGAACCATCTGCAGAAGCTAAATTTGATTCACCAGTTGAATCGGTTCAAGATTCACAACCTGAACCTCTCCTTGAAGAAGTTCAAACTCAACCAGAAATTCAACCTGTAGAATCTCAACCAGAAGCTACTTTTGATACTGTTCAACCTGAACAAACTCCTCAAGAAGCTAAATTTGATTCACCAGTTGAAACTATTCAAGAACCTCAAGTTTCTAGTGAACCTGAAGTAGTTGTTCAACCTAACTTTGAAGAACGAAAACCTGAAACAGTATTGGAAGAACCTCAAGCTGATGAAATTCAACCAGAAGCTAGTGAAGAAGAATCACTTGATTGA", "aa": "MEYKVDFLETEPKSLFDEKTTIVVESEPPFIQPDLSLELDSVNDVDKSLETKTTSVELNHEEIGNEFINLDVSEKEVQEQPTTQLETDSEFVLPTYQIVEDSFTESAETPNEFSSEQKDTLEFISQTQEVETSESNVPTVEQETKLFEHQDENNLFTPLPLDLTEIIESNALFDSKPDEKESSDSELQPTFKEIKLDSTVEVPQESSQVEATFDTVQPEAVFDEIKTQELQPEATTEVVFDDHFQPDVQPEQTPQEAKFDSPVEIPQESSQAEFHAEQISDEIKLEEKTEAVFDHQQLENQSEETVVTPTEVTAFEPETIETQLEPSSEDQPSEPALDQNHPEIVTAEVEQIFDGTKLEDLKLEEANFDNVENNEVQPKETEAEITFDETKELQQETSSEPLSTEELKSEATFDNVSEAESEAVFEKPQLETQTEKILEEEPKSEPVDQLITEASFDTVKHEAVFDKNQTQTEGLEEPQVSSEAEVVDQTTTDTVGEPEAVFDVQPEKTTEVKFDDVENQQKVISEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPVLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETVEQPEFSSEPTQQHVESEASFDEPNYDFDEPNYDFDQPSYDSDLQPSEPQYDVDEPNYDFDEPNYEIESKPSEPQFEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPLLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETIQEPQVSSEPEVVVQPNFEERKPETVLEEPQADEIQPEASEEESLD", "aa_hexdigest": "9844bd744fa8328635d85550869b10f5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 484939, "stop": 487947, "observations": { "mutation": [ 484959, 485001, 485022, 485130, 485154, 485184, 485190, 485244 ], "stop": [ 484959, 485001, 485022, 485130, 485154, 485184, 485190, 485244 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49429", "query_cov": 1.0, "subject_cov": 0.49566831683168316, "identity": 0.9990000000000001, "score": 1052.0, "evalue": 0.0, "gene_start": 1, "gene_end": 801, "reference_start": 636, "reference_end": 1436, "reference_sequence": "MPKTTKNKNKNTTPKSKTKKYLESANKKSVTKPKKEQDKVENLFDQPFLGEIKKNILKKTKSFNSKKKETVKSKSKSPIDFFDETKRGVFIVPPETDILSRRELNQKTVVNTVPNQTSSYPTINENKLVELNNQPETKVLETKKDSFTTTIREKKLNPEDSQAFWYIFVGDRKYGFWKNHTWVWLGYFDQLQRWNYFKVIETVEVPQEHAAFIKQRPADIDFWRPLVGNPNYGFVQNNTWIWKGFFDKKLNWIPDPVRFTEEALGHTDSLVDEIEKKTISEQPYWEQENDIVVTVFNTKSLASSLENELLLENSSEEQPVIEEVKPRRNEVIFRNPVTKLHFEKEKFEFLNPVKETNETIPLIEIVKEEVKVESEVEAPVEIEPEAACEPETTIPEVETVFVYEDDLKGLDSNQTQAGNVPEVETVFVYEDDLKGLDSIIKDDQQHDEIAKHVEHLSQDYSKEIKDSAKADLSNISDDIDSVWKEFGSFTDETQKSVEEKSQVDEIILDANNDFINESLFRDEVVNNIDSQINETVSEQQFEPTYSVNEFQQEFSEPVVSDEKIKETNSDESVNTDLTALFSEKLVNEVLLTNEYVDVNAPFSTETEVKVSSELPKSELVDEITFINNDPKPQEGLEYKVDFLETEPKSLFDEKTTIVVESEPPFIQPDLSLELDSVNDVDKSLETKTTSVELNHEEIGNEFINLDVSEKEVQEQPTTQLETDSEFVLPTYQIVEDSFTESAETPNEFSSEQKDTLEFISQTQEVETSESNVPTVEQETKLFEHQDENNLFTPLPLDLTEIIESNALFDSKPDEKESSDSELQPTFKEIKLDSTVEVPQESSQVEATFDTVQPEAVFDEIKTQELQPEATTEVVFDDHFQPDVQPEQTPQEAKFDSPVEIPQESSQAEFHAEQISDEIKLEEKTEAVFDHQQLENQSEETVVTPTEVTAFEPETIETQLEPSSEDQPSEPALDQNHPEIVTAEVEQIFDGTKLEDLKLEEANFDNVENNEVQPKETEAEITFDETKELQQETSSEPLSTEELKSEATFDNVSEAESEAVFEKPQLETQTEKILEEEPKSEPVDQLITEASFDTVKHEAVFDKNQTQTEGLEEPQVSSEAEVVDQTTTDTVGEPEAVFDVQPEKTTEVKFDDVENQQKVISEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPVLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETVEQPEFSSEPTQQHVESEASFDEPNYDFDEPNYDFDQPSYDSDLQPSEPQYDVDEPNYDFDEPNYEIESKPSEPQFEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPLLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETIQEPQVSSEPEVVVQPNFEERKPETVLEEPQADEIQPEASEEESLDWELLVGNNSYGHYEPDGEWVWAGFFGDDQKWNKDATVKWARERDYLPLIGDEVYGRYNNKGEWIWYGFYDESGDWVLVDEQWKNRQPRINEAPKFWEKLIGNEEYGYYEDNEWNWYDGEFDSEGNWLVFQSEETENLNEDITKDIPALEGYDIDSIDADEWLSQFSADDAKDVFGSNDKK" }, "paralog": false, "identity": 0.9890219560878244, "score": 1460.0, "evalue": 0.0, "description": "Internal stop codon at 484959, 485001, 485022, 485130, 485154, 485184, 485190, 485244. Nonsense mutation around 484959, 485001, 485022, 485130, 485154, 485184, 485190, 485244.", "psc": { "uniref90_id": "UniRef90_Q49429", "product": "Protein P200", "uniref50_id": "UniRef50_Q49429", "go_ids": [ "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49429", "UniRef:UniRef50_Q49429", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q49429", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49429" ], "product": "Protein P200" } }, "id": "KKNAAMEMLJ_874", "locus": "GCA000027325_00874" }, { "type": "cds", "sequence": "contig_1", "start": 488009, "stop": 488176, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCATTATTAGCATCTAAAATAATTTCATCTACTTGTGATTTTTCTTCTACACTTTTTTGTGTTTCATCAGTAAAAGAACCGAATTCTTTTCAAACTGAATCAATATCATCAGAAATATTAGATAAATCTGCTTTAGCACTATCTTTTATTTCTTTAGAATAA", "aa": "MKSLLASKIISSTCDFSSTLFCVSSVKEPNSFQTESISSEILDKSALALSFISLE", "aa_hexdigest": "092228fd981c62e73b5e6ecaad045e11", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5921.717399999998, "isoelectric_point": 4.8005872726440435 }, "id": "KKNAAMEMLJ_875", "locus": "GCA000027325_00875" }, { "type": "cds", "sequence": "contig_1", "start": 488474, "stop": 488758, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCATTAGTTTCTTTAACTGGATTTAAGAATTCAAATTTTTCTTTTTCAAAATGTAATTTAGTTACTGGATTACGGAAGATAACTTCATTTCTTCTTGGTTTAACTTCTTCAATTACCGGCTGCTCTTCACTTGAATTTTCAAGTAATAGTTCGTTTTCTAGAGAAGAAGCTAAGCTCTTGGTATTAAATACTGTAACAACAATGTCATTTTCCTGTTCTCAGTATGGTTGTTCAGAAATAGTCTTTTTCTCTATTTCATCAACTAAGCTATCAGTATGA", "aa": "MVSLVSLTGFKNSNFSFSKCNLVTGLRKITSFLLGLTSSITGCSSLEFSSNSSFSREEAKLLVLNTVTTMSFSCSQYGCSEIVFFSISSTKLSV", "aa_hexdigest": "4efbb505a0c6d021d68f16798ce2e917", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10122.533399999993, "isoelectric_point": 8.836504173278808 }, "id": "KKNAAMEMLJ_876", "locus": "GCA000027325_00876" }, { "type": "cds", "sequence": "contig_1", "start": 489058, "stop": 489552, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAAACAACAAAGAATAAAAACAAAAACACCACCCCTAAATCAAAAACCAAAAAGTATTTAGAATCAGCTAACAAGAAGAGTGTTACTAAACCAAAAAAAGAACAAGATAAAGTTGAAAATCTTTTTGATCAACCTTTTTTAGGTGAAATCAAAAAAAACATTCTCAAGAAAACAAAGTCTTTTAATAGTAAGAAAAAGGAAACAGTTAAATCAAAGAGCAAATCACCTATAGATTTTTTTGATGAAACCAAGCGCGGTGTTTTTATTGTTCCACCTGAAACAGATATCTTAAGTAGAAGAGAACTTAATCAAAAAACTGTTGTTAATACAGTTCCAAATCAAACAAGCAGTTATCCAACCATTAATGAAAATAAGTTGGTTGAATTAAACAATCAACCAGAAACCAAAGTTCTAGAAACAAAAAAAGATAGTTTTACAACAACTATTAGAGAAAAAAAATTAAATCCAGAAGATTCACAAGCGTTCTGA", "aa": "MPKTTKNKNKNTTPKSKTKKYLESANKKSVTKPKKEQDKVENLFDQPFLGEIKKNILKKTKSFNSKKKETVKSKSKSPIDFFDETKRGVFIVPPETDILSRRELNQKTVVNTVPNQTSSYPTINENKLVELNNQPETKVLETKKDSFTTTIREKKLNPEDSQAF", "aa_hexdigest": "4710bbf8d9536695c6d5ba638c063d09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18893.3926, "isoelectric_point": 9.87360706329346 }, "id": "KKNAAMEMLJ_877", "locus": "GCA000027325_00877" }, { "type": "cds", "sequence": "contig_1", "start": 489839, "stop": 490042, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07650.23" ], "nt": "TTGTTAAAAATTCACCTAGTAATTAGTGTTCCAAAATTAAGCCAAAAAAAGATTATCATTGGAAAAAATGCAGAAATGATCAAAGCAATTGGGATAGCAACTCGCAAAAAACTATTAAATCATTTTGACTGTGATATTTTTATTGATATTTTTGTAAAAACAGAAAAACAAAAATTGCCTGTTTATAGTTTTCTTTCTAAATAA", "aa": "MLKIHLVISVPKLSQKKIIIGKNAEMIKAIGIATRKKLLNHFDCDIFIDIFVKTEKQKLPVYSFLSK", "aa_hexdigest": "fe21dd93fe1b44978f05834f70f587a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07650.23", "name": "KH_2", "length": 58, "aa_cov": 0.8059701492537313, "hmm_cov": 0.9827586206896551, "evalue": 5.369584697762552e-11, "score": 37.331119537353516, "start": 4, "stop": 57 } ], "seq_stats": { "molecular_weight": 7697.348799999998, "isoelectric_point": 9.945747184753419 }, "id": "KKNAAMEMLJ_878", "locus": "GCA000027325_00878" }, { "type": "cds", "sequence": "contig_1", "start": 490232, "stop": 490711, "strand": "-", "frame": 2, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "EC:3.6.5.-", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0019843", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_P47627", "UniRef:UniRef90_P47627" ], "nt": "ATGAAAGTTTTAAAAGTTGGTGTTTTAGGACCTACTAATGCAGGAAAATCAACGCTAATTAATTTTTTACATAACGATGATAGTCTGATGGTATCATCCATGAATAACACAACGCTTCTTTCTATTTCCACTGAGGTAATTAACCAAGCTAATAAAAATATTGTTTTTATTGATGTCCCTGGTTTTACTGAGAAAAAACATTCAAATTATGAATTAATAACTAAAGAAATTCGCAAAGCATTAAGTGGAATTGATGTATTACTTTTAGTTGTTAGAAGTGATCAGAATAACAAGATAGAATTTTTAAAAACTCAATTACAACAATTAAAGCGCTATCAAAATTTAACAAGAATTTTTTTAATTAATAAATTTCATCAAAAAAGTTTAAGTGAAGTTAATAAAGCAATTATTTTAGAAGAGTTTAAACCACAAAAAACAATTGAAATAAACTTGCTTAAGTTTGATAAAAACCTCTTTTGA", "aa": "MKVLKVGVLGPTNAGKSTLINFLHNDDSLMVSSMNNTTLLSISTEVINQANKNIVFIDVPGFTEKKHSNYELITKEIRKALSGIDVLLLVVRSDQNNKIEFLKTQLQQLKRYQNLTRIFLINKFHQKSLSEVNKAIILEEFKPQKTIEINLLKFDKNLF", "aa_hexdigest": "fe69406db23df748a8201ca25f056780", "start_type": "ATG", "rbs_motif": null, "genes": [ "era" ], "truncated": "5-prime", "pseudogene": { "start": 489929, "stop": 490711, "observations": { "mutation": [ 490234 ], "stop": [ 490234 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47627", "query_cov": 1.0, "subject_cov": 0.5482758620689655, "identity": 1.0, "score": 300.0, "evalue": 8.66e-101, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MKVLKVGVLGPTNAGKSTLINFLHNDDSLMVSSMNNTTLLSISTEVINQANKNIVFIDVPGFTEKKHSNYELITKEIRKALSGIDVLLLVVRSDQNNKIEFLKTQLQQLKRYQNLTRIFLINKFHQKSLSEVNKAIILEEFKPQKTIEINLLKFDKNLFWSIFKQVELRYNIFRKDINFIDANNDDFKILEGLREQIIFYCKNEIPHIARIEIIEKSFNKEKNLLKIHLVISVPKLSQKKIIIGKNAEMIKAIGIATRKKLLNHFDCDIFIDIFVKTEKQKLPVYSFLSK" }, "paralog": false, "identity": 0.9961538461538462, "score": 498.0, "evalue": 1.25e-181, "description": "Internal stop codon at 490234. Nonsense mutation around 490234.", "psc": { "uniref90_id": "UniRef90_P47627", "gene": "era", "product": "GTPase Era", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P47627", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0019843", "GO:0043024", "GO:0070181" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47627", "EC:3.6.5.-", "UniRef:UniRef50_P47627", "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0019843", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47627", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47627" ], "product": "GTPase Era" } }, "id": "KKNAAMEMLJ_879", "locus": "GCA000027325_00879" }, { "type": "cds", "sequence": "contig_1", "start": 490840, "stop": 491163, "strand": "-", "frame": 3, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "EC:3.1.-.-", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47628", "UniRef:UniRef90_P47628" ], "nt": "TTGAAGTCATCTTTTAGCATTAATTCAAGCCGCCTTTTTAAGCGCTTTTTTGGTAAAAATTACCAAGCAGGAGTCGAACTTTGTTTCCAAATTATTAAAAGCAGTTTAAACCTATCGTTTGACCCAGAATTTGCGCTTTTAATTGTTTCCTGTTCAAAAATGAAAAAACTGAACAAACAGTTTTTAAAACGTAGAGGTTGTACTGATGTAATTAGTTTGTGCTATAACGAAACTGAAACCGGTTTTTCACTAGCAATCGGCGAAATTTTTTTGTGTCCTAAATACATCTTTAAAAAAGCTAAAGAGATTGGTTGTAACAATTGA", "aa": "MKSSFSINSSRLFKRFFGKNYQAGVELCFQIIKSSLNLSFDPEFALLIVSCSKMKKLNKQFLKRRGCTDVISLCYNETETGFSLAIGEIFLCPKYIFKKAKEIGCNN", "aa_hexdigest": "09db2ba6b472e48feac7092f8ce790c2", "start_type": "TTG", "rbs_motif": null, "genes": [ "ybeY" ], "truncated": "5-prime", "pseudogene": { "start": 490699, "stop": 491163, "observations": { "mutation": [ 490710, 490842 ], "stop": [ 490710, 490842 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47628", "query_cov": 1.0, "subject_cov": 0.6948051948051948, "identity": 1.0, "score": 218.0, "evalue": 1.45e-71, "gene_start": 1, "gene_end": 107, "reference_start": 1, "reference_end": 107, "reference_sequence": "MKSSFSINSSRLFKRFFGKNYQAGVELCFQIIKSSLNLSFDPEFALLIVSCSKMKKLNKQFLKRRGCTDVISLCYNETETGFSLAIGEIFLCPKYIFKKAKEIGCNNWFLLTRCLIHGILHLFEFNHEESEAFESVTMFFQDAIHEEILSVWKF" }, "paralog": false, "identity": 0.9805194805194806, "score": 304.0, "evalue": 6.54e-108, "description": "Internal stop codon at 490710, 490842. Nonsense mutation around 490710, 490842.", "psc": { "uniref90_id": "UniRef90_P47628", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47628", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47628", "EC:3.1.-.-", "UniRef:UniRef50_P47628", "COG:COG0319", "COG:J", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47628", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47628" ], "product": "Endoribonuclease YbeY" } }, "id": "KKNAAMEMLJ_880", "locus": "GCA000027325_00880" }, { "type": "cds", "sequence": "contig_1", "start": 491201, "stop": 491395, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG389", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47629", "UniRef:UniRef90_P47629" ], "nt": "ATGTTTAAAAATGAAAAAGATGAAAATCTTTTTAACAACGTTTCATGGACTAATTTTCAAGCAGAAACAACAGAAAAATCAGCAAAAAAAGCTTTTAGACTTTACAAAAAGAAAATTTCTACATCAGAAATTTCATCAGAATTGAACAAAAATCTAGTAAAGGTAGATTTAAGTGTTACTCTAGAACAGGGCTGA", "aa": "MFKNEKDENLFNNVSWTNFQAETTEKSAKKAFRLYKKKISTSEISSELNKNLVKVDLSVTLEQG", "aa_hexdigest": "3811cb96904f9a648d7c9e2d2717cc78", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 491147, "stop": 491530, "observations": { "mutation": [ 491203, 491422 ], "stop": [ 491203, 491422 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47629", "query_cov": 1.0, "subject_cov": 0.5039370078740157, "identity": 1.0, "score": 119.0, "evalue": 1.46e-33, "gene_start": 1, "gene_end": 64, "reference_start": 46, "reference_end": 109, "reference_sequence": "MKQKILGITIAFIILLLTTVAILFSVKVKQYLNVNLWTTQQQNFLMFKNEKDENLFNNVSWTNFQAETTEKSAKKAFRLYKKKISTSEISSELNKNLVKVDLSVTLEQGWYNITIMLPSKDLFEVIF" }, "paralog": false, "identity": 0.984251968503937, "score": 238.0, "evalue": 1.08e-82, "description": "Internal stop codon at 491203, 491422. Nonsense mutation around 491203, 491422.", "psc": { "uniref90_id": "UniRef90_P47629", "product": "Uncharacterized protein MG389", "uniref50_id": "UniRef50_P47629", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47629", "UniRef:UniRef50_P47629", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47629", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47629" ], "product": "Uncharacterized protein MG389" } }, "id": "KKNAAMEMLJ_881", "locus": "GCA000027325_00881" }, { "type": "cds", "sequence": "contig_1", "start": 491420, "stop": 491530, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCAGAAAATTTTAGGAATAACAATTGCTTTTATTATTTTGCTTTTAACAACAGTTGCTATCTTGTTTTCAGTAAAGGTAAAACAATATCTTAATGTAAATCTTTGA", "aa": "MKQKILGITIAFIILLLTTVAILFSVKVKQYLNVNL", "aa_hexdigest": "46036968c72f159cecceacf64166a32e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4061.0538000000006, "isoelectric_point": 10.176350593566895 }, "id": "KKNAAMEMLJ_882", "locus": "GCA000027325_00882" }, { "type": "cds", "sequence": "contig_1", "start": 491534, "stop": 491881, "strand": "-", "frame": 2, "gene": null, "product": "ABC-type enterochelin transport system, ATPase component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N885" ], "nt": "ATGCAAACAGAAGCAAATGATTTTGGCTTTAGTGAGGCTTTATTTAATAATCAGAATCCACATATCTATCAGTTACTTTTTGATGCAGGTGTTCAAAATCAAACAAAGTTAAGTTCAGGACAAAAACAAATTTTACAACTATTCTTACTTAGCAACATTAAGAATAAGGTGATCTTACTTGATGAATGCATGAATGCAATAGCACCTGAAATTAAAAACCGTGTTTATCAGCTATTAGTAAAACCACTTACTTTAAATAATTTTGTAGTCTTAGTTGAGCATGATCTGAGTTTTGCAAGTGAAGCTCAAAACAAGATTAACCTAACTAATTACTTAAGAAATAGTTAA", "aa": "MQTEANDFGFSEALFNNQNPHIYQLLFDAGVQNQTKLSSGQKQILQLFLLSNIKNKVILLDECMNAIAPEIKNRVYQLLVKPLTLNNFVVLVEHDLSFASEAQNKINLTNYLRNS", "aa_hexdigest": "d9e4a607d87bdad315209b09af887035", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAV5N885", "query_cov": 0.8695652173913043, "subject_cov": 0.9803921568627451, "identity": 0.67, "score": 132.0, "evalue": 3.65e-38, "valid": false, "product": "ABC-type enterochelin transport system, ATPase component", "uniref50_id": "UniRef50_A0AAV5N885" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N885", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N885" ], "product": "ABC-type enterochelin transport system, ATPase component" }, "genes": [], "id": "KKNAAMEMLJ_883", "locus": "GCA000027325_00883" }, { "type": "cds", "sequence": "contig_1", "start": 492143, "stop": 492823, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACAAAAAAGCTTTGTTTACTTTAGTAATAAATCACCAAATCAATTAATAGAACGACAATTTTATTTAAAAGAAGTTCTTTCCTTTTTTAACTTTTATATTCCCAATTTAATTATTAGTTGTGTTGTTGCACTAATTATCGGTGTTTTAATAGGAATTAATCAATTAGAATTTTTATTGATTGCCATTGCACAAATAGTAGTCAATGCTGGCTTATTTTGTTATGACTTCTTTTTTACTAAAAAAATTACAAAGAAGGAAATTCCTTATGTTGAATTGCAAAACAAAATTAGTTTACAGCTAGATGAAAATTTGCGTGAAGAACAAAATAAAAAGCGCTTTAATTTCTTAATGTTGAATTTTCGTAAAGCACTTTTACAAAATCAAAACATTAACAACCAGAAGGAAATTAATCGTCTAACTATAGAAAACATTAAAAGCTTCTTCCAACAAGGTTTTGATTTTGCTATATTAGGTTTGGGGGTGATTGGTATTATTGAACAGCGTTATCAATTGAGCTTTTTATTCTATGTTTTTGGTATCCAATCACTTTTTTCAACTTATGCTACAAGAATTGTTCAATTTGGTGCTGCAATAAACATTTATCACTATTGTCGTGAGAAATTAGTTAACTTGTTTATAGAAACCAAAAAGGATGAGGGTATTAAAGTAAATTGA", "aa": "MQQKSFVYFSNKSPNQLIERQFYLKEVLSFFNFYIPNLIISCVVALIIGVLIGINQLEFLLIAIAQIVVNAGLFCYDFFFTKKITKKEIPYVELQNKISLQLDENLREEQNKKRFNFLMLNFRKALLQNQNINNQKEINRLTIENIKSFFQQGFDFAILGLGVIGIIEQRYQLSFLFYVFGIQSLFSTYATRIVQFGAAINIYHYCREKLVNLFIETKKDEGIKVN", "aa_hexdigest": "2ba9b9a482bff619f5503df6163b3eb5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26553.785900000013, "isoelectric_point": 9.24955310821533 }, "id": "KKNAAMEMLJ_884", "locus": "GCA000027325_00884" }, { "type": "cds", "sequence": "contig_1", "start": 493223, "stop": 493516, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATCATTTATCAAGAACAACAGAATGAGTGTGGAATTTGCGTAATTGGTATGCTTGCCAATGCAATCCATGATGAAAAGTATGTTCATGATGAATTACTAGAACAAATTAACTTACCACCAAATGGCTTAAGCTTATTTGAAATGGAAAGTTATGGCAAAAAGTTTGGTCTTGAAATCAACAGTTATCAATTAACATTTCAAGAACTAAAAGAACTTGATAGCAAATTCATAATAGTTCATTTTAAAGATCACTTTGTCATAGTTAAAAACAAACATGAAAATAGTTGA", "aa": "MKIIYQEQQNECGICVIGMLANAIHDEKYVHDELLEQINLPPNGLSLFEMESYGKKFGLEINSYQLTFQELKELDSKFIIVHFKDHFVIVKNKHENS", "aa_hexdigest": "2f88fd873904c40c1cdda9a37a3c97f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11357.908999999987, "isoelectric_point": 5.241998863220213 }, "id": "KKNAAMEMLJ_885", "locus": "GCA000027325_00885" }, { "type": "cds", "sequence": "contig_1", "start": 493624, "stop": 493857, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCACCCTGATTATTTAAAACCTTTACAGCTAACAAAACTTGCTGATTTGCAAAATTCTACTAGTGCAAGAGGTGCTGGATCTTCAAGAGCAGCTTGTTTCCTTGCAGAATTTAGAGAAGGTGTATCTTTGATCCATTGTGATATTGCATCAACTGCTTCCATTGAGAACCTTGGACAAGGTGTTTTAGTGAGAACCTTGTACGAACGTGCTAGTCAGCTTGCAAATAAATAA", "aa": "MHPDYLKPLQLTKLADLQNSTSARGAGSSRAACFLAEFREGVSLIHCDIASTASIENLGQGVLVRTLYERASQLANK", "aa_hexdigest": "35eaeb6e96a5625f289f9cfdcb7639f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 74, "aa_cov": 0.8571428571428571, "hmm_cov": 0.972972972972973, "evalue": 1.404292822556103e-10, "score": 35.99742126464844, "start": 2, "stop": 67 } ], "seq_stats": { "molecular_weight": 8305.357199999993, "isoelectric_point": 7.827573966979982 }, "id": "KKNAAMEMLJ_886", "locus": "GCA000027325_00886" }, { "type": "cds", "sequence": "contig_1", "start": 493912, "stop": 494967, "strand": "-", "frame": 3, "gene": "pepA", "product": "leucyl aminopeptidase", "db_xrefs": [ "COG:COG0260", "COG:E", "EC:3.4.11.1", "GO:0005737", "GO:0006508", "GO:0008233", "GO:0030145", "GO:0070006", "SO:0001217", "UniRef:UniRef50_P47631", "UniRef:UniRef90_P47631" ], "nt": "ATGAGAATAAATAAACCTTTTAGTGATGATAGCAACACAGTTGTTTTTGTGAGTTCAAAAACATATGGTGTAAAAGAAGAAGCTGCACATAATCCTAATGTTGAATTTGGTGTTGTTTTACCAACTGATTTTCCTGCTTTCAACCGTGCTTTAGTTCAATTTCTTAAAAGAAAGAAAACCAAATTAAACATTAATCTTGACAGTCTTATAGAACTTTATAAGAAAAATGAAAATAGTGGTTGTTTTCATACTGCGATAAAAACTGTTATTACAAGTGTTACTTTTTGTGAAACTACTCCTTTCACAATGAAAACCAAACCTGAAAAAAATGTTGAAGTTGCTGTTCAATGTGCTGTTGAATATCACAACTTAGTTAAAGAATATGAAACAGTAGGCGAATATGTTAACCTAGCAAGAGAATTACAAGACACTCCTTCAGATCTACTTTATTCAGAAGTATTTGTTAAACATTTTGAAAAGGCTGCAAGTAAATTGCCTGTAAAAATAAAAGTTCTTAAACAATCAGATCTAATTAAGAAAAAAATGGGTTTACTTTTAGGGGTTAATCAAGGCTCTGAAAGAGAAGCACGTTTACTTGTTATTAGTTATCAAGCTAATAAAAATTCCAAAGAAAAACTTGCTTTTGTAGGGAAAGGAATTACTTATGATTCAGGCGGAATGAACATTAAAACAGGTGATTATATGCGTGGCATGAAATATGACATGAGTGGTGCGGCTATAGTATGTTCTACTGTTTTGGCATTAGCTAAAAACAAGGTTAAAACCAATGTTGTTGCAGTAGCAGCTCTTACTGAAAATCTTCCTGGTGCTAAGGCGCAACGTCCTGATGACATTAAGATAGCATACAATGGTAAAAGTGTTGAAATAGATAACACTGATGCTGAAGGAAGATTGGTTTTAGCTGATGCTATTACATATGCTGCTAAGGATTTAGCTGCTACACATATTATTGATGTAGCAACCCTTACTGGTTTAATGTCATACATATTGAGTACTACCTATACAGGTATTTTCAGTACTTGTGATCACCAGTGA", "aa": "MRINKPFSDDSNTVVFVSSKTYGVKEEAAHNPNVEFGVVLPTDFPAFNRALVQFLKRKKTKLNINLDSLIELYKKNENSGCFHTAIKTVITSVTFCETTPFTMKTKPEKNVEVAVQCAVEYHNLVKEYETVGEYVNLARELQDTPSDLLYSEVFVKHFEKAASKLPVKIKVLKQSDLIKKKMGLLLGVNQGSEREARLLVISYQANKNSKEKLAFVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGAKAQRPDDIKIAYNGKSVEIDNTDAEGRLVLADAITYAAKDLAATHIIDVATLTGLMSYILSTTYTGIFSTCDHQ", "aa_hexdigest": "d65736e1226c5a8f9886944768b8f03f", "start_type": "ATG", "rbs_motif": null, "genes": [ "pepA" ], "truncated": "5-prime", "pseudogene": { "start": 493624, "stop": 494967, "observations": { "mutation": [ 493869, 493914 ], "stop": [ 493869, 493914 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47631", "query_cov": 1.0, "subject_cov": 0.785234899328859, "identity": 1.0, "score": 672.0, "evalue": 2.42e-242, "gene_start": 1, "gene_end": 351, "reference_start": 1, "reference_end": 351, "reference_sequence": "MRINKPFSDDSNTVVFVSSKTYGVKEEAAHNPNVEFGVVLPTDFPAFNRALVQFLKRKKTKLNINLDSLIELYKKNENSGCFHTAIKTVITSVTFCETTPFTMKTKPEKNVEVAVQCAVEYHNLVKEYETVGEYVNLARELQDTPSDLLYSEVFVKHFEKAASKLPVKIKVLKQSDLIKKKMGLLLGVNQGSEREARLLVISYQANKNSKEKLAFVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGAKAQRPDDIKIAYNGKSVEIDNTDAEGRLVLADAITYAAKDLAATHIIDVATLTGLMSYILSTTYTGIFSTCDHQWESFKKAACSAGEPVWRLPMHPDYLKPLQLTKLADLQNSTSARGAGSSRAACFLAEFREGVSLIHCDIASTASIENLGQGVLVRTLYERASQLANK" }, "paralog": false, "identity": 0.9955257270693513, "score": 868.0, "evalue": 7.95e-322, "description": "Internal stop codon at 493869, 493914. Nonsense mutation around 493869, 493914.", "psc": { "uniref90_id": "UniRef90_P47631", "gene": "pepA", "product": "leucyl aminopeptidase", "ec_ids": [ "3.4.11.1" ], "uniref50_id": "UniRef50_P47631", "cog_id": "COG0260", "cog_category": "E", "go_ids": [ "GO:0005737", "GO:0030145", "GO:0070006", "GO:0008233", "GO:0006508" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47631", "EC:3.4.11.1", "UniRef:UniRef50_P47631", "COG:COG0260", "COG:E", "GO:0005737", "GO:0030145", "GO:0070006", "GO:0008233", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47631", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47631" ], "product": "Probable cytosol aminopeptidase" } }, "id": "KKNAAMEMLJ_887", "locus": "GCA000027325_00887" }, { "type": "cds", "sequence": "contig_1", "start": 494984, "stop": 496615, "strand": "-", "frame": 2, "gene": "groL", "product": "chaperonin GroEL", "db_xrefs": [ "COG:COG0459", "COG:O", "EC:5.6.1.7", "GO:0005524", "GO:0009408", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0051085", "GO:0140662", "GO:1990220", "RefSeq:WP_009885631.1", "SO:0001217", "UniParc:UPI0000557C61", "UniRef:UniRef100_P47632", "UniRef:UniRef50_P47632", "UniRef:UniRef90_P47632" ], "nt": "ATGGCAAAGGAATTAATCTTTGGTAAAGATGCGAGAACCCGCTTGTTGCAGGGTATTAATAAGATAGCAAATGCTGTTAAAGTAACAGTAGGTCCTAAAGGCCAAAATGTTATTTTAGAGAGAAAATTTGCAAACCCATTAATTACTAACGATGGGGTTACAATCGCAAAAGAAATAGAACTTAGTGATCCAGTTGAAAATATTGGTGCTAAGGTTATTTCAGTTGCTGCAGTGTCAACTAATGACATTGCTGGGGATGGTACAACAACAGCTACCATATTAGCACAAGAAATGACAAACCGTGGTATTGAAATTATCAATAAAGGTGCTAATCCTGTTAACATCCGCAGGGGTATTGAAGATGCAAGCTTACTTATTATTAAAGAACTTGAAAAGTACTCTAAAAAAATTAATACTAACGAAGAGATAGAACAAGTTGCAGCTATCTCTTCAGGTTCTAAAGAAATTGGTAAACTGATCGCTCAAGCAATGGCTTTAGTTGGTAAAAATGGCGTGATAACAACTGATGATGCAAAAACCATTAATACAACATTAGAAACCACTGAAGGAATTGAATTTAAAGGAACATATGCATCACCTTATATGGTTAGTGATCAAGAAAAAATGGAAGTTGTTTTAGAACAACCTAAAATCTTAGTAAGCTCTTTAAAAATTAACACAATTAAAGAAATTCTTCCGCTTTTAGAAGGTAGTGTTGAAAATGGTAATCCATTATTAATTGTTGCACCTGACTTTGCAGAAGAAGTTGTTACTACTTTAGCAGTTAATAAACTCAGGGGCACCATTAATGTTGTTGCTGTTAAATGTAATGAATATGGTGAACGTCAAAAAGCAGCTTTAGAAGATTTAGCAATTAGTAGTGGAACCTTAGCATATAATAACGAAATTAATAGTGGTTTTAAAGATGTTACTGTTGATAATTTAGGTGATGCTAGAAAGGTTCAAATAGCTAAAGAAAAAACTACTGTTATTGGTGGTAAAGGCAATAAGGATAAAATCAAAAAGCATGTTGAACTTCTAAACGGAAGATTAAAACAAACCACTGACAAGTATGATTCTGATTTAATTAAAGAAAGAATTGCTTATTTAAGTCAAGGTGTTGCTGTTATCCGTGTTGGTGGTGCAACTGAACTCGCACAAAAAGAATTAAAACTCAGAATCGAAGACGCTTTAAATTCCACCAAAGCTGCAGTTGAAGAAGGGATTATCGCTGGAGGTGGTGTTGGTTTATTAAATGCTTCTTGTGTTTTAACTAACAGTAAACTAAAAGAACGATATGAAAATGAAACTAGTGTTGAAAACATTAAAGAAATCCTACTTGGTTTTGAAATTGTGCAAAAGTCTCTAGAAGCACCAGCGCGTCAAATTATTCAAAACTCAGGAGTTGACCCAGTTAAAATTCTCAGTGAATTGAAAAATGAAAAAACTGGTGTTGGCTTTGATGCTGAGACTAAAAAGAAGGTTGATATGATTGCAAATGGAATCATTGATCCCACCAAAGTAACTAAAACTGCACTTGAAAAAGCTGCTTCTGTAGCTAGTTCATTAATTACTACTAATGTTGCTGTGTATGATGTTAAAGAGAGAAAAGATAACTCCTTTTCAGAATAA", "aa": "MAKELIFGKDARTRLLQGINKIANAVKVTVGPKGQNVILERKFANPLITNDGVTIAKEIELSDPVENIGAKVISVAAVSTNDIAGDGTTTATILAQEMTNRGIEIINKGANPVNIRRGIEDASLLIIKELEKYSKKINTNEEIEQVAAISSGSKEIGKLIAQAMALVGKNGVITTDDAKTINTTLETTEGIEFKGTYASPYMVSDQEKMEVVLEQPKILVSSLKINTIKEILPLLEGSVENGNPLLIVAPDFAEEVVTTLAVNKLRGTINVVAVKCNEYGERQKAALEDLAISSGTLAYNNEINSGFKDVTVDNLGDARKVQIAKEKTTVIGGKGNKDKIKKHVELLNGRLKQTTDKYDSDLIKERIAYLSQGVAVIRVGGATELAQKELKLRIEDALNSTKAAVEEGIIAGGGVGLLNASCVLTNSKLKERYENETSVENIKEILLGFEIVQKSLEAPARQIIQNSGVDPVKILSELKNEKTGVGFDAETKKKVDMIANGIIDPTKVTKTALEKAASVASSLITTNVAVYDVKERKDNSFSE", "aa_hexdigest": "5502f1565b9d7b37257e1363472a1713", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557C61", "ncbi_nrp_id": "WP_009885631.1", "uniref100_id": "UniRef100_P47632", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557C61", "RefSeq:WP_009885631.1", "UniRef:UniRef100_P47632" ] }, "ips": { "uniref100_id": "UniRef100_P47632", "uniref90_id": "UniRef90_P47632" }, "psc": { "uniref90_id": "UniRef90_P47632", "gene": "groL", "product": "chaperonin GroEL", "ec_ids": [ "5.6.1.7" ], "uniref50_id": "UniRef50_P47632", "cog_id": "COG0459", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0009408", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0051085", "GO:0140662", "GO:1990220" ] }, "pscc": { "uniref50_id": "UniRef50_P47632", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47632" ], "product": "Chaperonin GroEL" }, "genes": [ "groL" ], "id": "KKNAAMEMLJ_888", "locus": "GCA000027325_00888" }, { "type": "cds", "sequence": "contig_1", "start": 496628, "stop": 496960, "strand": "-", "frame": 2, "gene": "groES", "product": "co-chaperone GroES", "db_xrefs": [ "COG:COG0234", "COG:O", "GO:0005524", "GO:0005737", "GO:0044183", "GO:0046872", "GO:0051082", "GO:0051085", "GO:0051087", "RefSeq:WP_010869464.1", "SO:0001217", "UniParc:UPI0000127634", "UniRef:UniRef100_P47633", "UniRef:UniRef50_P47633", "UniRef:UniRef90_P47633" ], "nt": "ATGAACATAACGCCAATTCATGACAACGTCTTGGTTTCACTTGTGGAATCAAACAAAGAAGAAGTCTCAAAAAAAGGGATTATTACCTCATTGGCAAGTAATGATAAAAGCGATGCTAATGCTAATAAAGGGATTGTAATTGCTCTTGGTGCTGGTCCTGCATATGGCAAAACAGAAAAACCAAAATATGCTTTTGGTGTTGGTGATATTATTTACTTTAAGGAGTATAGTGGTATCTCTTTTGAGAATGAGGGAAACAAGTACAAAATTATTGGATTTGAGGATGTACTTGCCTTTGAAAAACCAGAAAGTGGTAAGCAAAGAAAAAGATAA", "aa": "MNITPIHDNVLVSLVESNKEEVSKKGIITSLASNDKSDANANKGIVIALGAGPAYGKTEKPKYAFGVGDIIYFKEYSGISFENEGNKYKIIGFEDVLAFEKPESGKQRKR", "aa_hexdigest": "00e270f14d81d7b2ac3f76a93d713102", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000127634", "ncbi_nrp_id": "WP_010869464.1", "uniref100_id": "UniRef100_P47633", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000127634", "RefSeq:WP_010869464.1", "UniRef:UniRef100_P47633" ] }, "ips": { "uniref100_id": "UniRef100_P47633", "uniref90_id": "UniRef90_P47633" }, "psc": { "uniref90_id": "UniRef90_P47633", "gene": "groES", "product": "co-chaperone GroES", "uniref50_id": "UniRef50_P47633", "cog_id": "COG0234", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0044183", "GO:0051087", "GO:0051082", "GO:0051085" ] }, "pscc": { "uniref50_id": "UniRef50_P47633", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47633" ], "product": "Co-chaperonin GroES" }, "genes": [ "groES" ], "id": "KKNAAMEMLJ_889", "locus": "GCA000027325_00889" }, { "type": "cds", "sequence": "contig_1", "start": 497086, "stop": 497412, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "GTGTCAAAAGGTACTGAAACCCACTTATTTTCATTAGTGGTTGGTAATGGTAAAGATGTTGCGTTGTGGTTACAAAAAGCTAACATTGTTTTGAATATGAATACAATCCCTTTTGAAACAAAATCTGCTTTTAGTCCTTCAGGTATTAGACTTGGAACTCCTGCAATGACAACCAGAGGTTTTAAAACTAATGACTTTATTTTTGTTGCCAGTTTGATTGATAAGGTTATTAAAAGTAATGGTAATCAAAAGGTAATTAGTCAAACAAAAACAGCTGTTTTAAATCTCTTAAAACGCTTTCCGCTCTATAAGGGTTTAGCTTATTAA", "aa": "MSKGTETHLFSLVVGNGKDVALWLQKANIVLNMNTIPFETKSAFSPSGIRLGTPAMTTRGFKTNDFIFVASLIDKVIKSNGNQKVISQTKTAVLNLLKRFPLYKGLAY", "aa_hexdigest": "316b9f1848202e548b155262d8a54a9e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 76, "aa_cov": 0.6666666666666666, "hmm_cov": 0.9868421052631579, "evalue": 1.1545520529916897e-17, "score": 58.72493362426758, "start": 2, "stop": 73 } ], "seq_stats": { "molecular_weight": 11863.782799999984, "isoelectric_point": 10.197689628601072 }, "id": "KKNAAMEMLJ_890", "locus": "GCA000027325_00890" }, { "type": "cds", "sequence": "contig_1", "start": 497437, "stop": 498306, "strand": "-", "frame": 3, "gene": null, "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E8WS34" ], "nt": "ATGTTTTCAAAGGTAAGACTTTTACTTAATAAAGAGTTACAACGTCAAAGAGAAAACATTTGTTTAATTGCTTCAGAAAATTACGTTAGCCAAGACATATTAGCTGTAACTGGTTCAGTATTAACAAATAAATATGCAGAAGGCTATCCCAGTAAACGTTTTTATCAAGGCTGTGAAGTTGTTGATGAATCTGAAAACTTAGCCATTGAAAGTTGCAAAACTTTATTTGGAGCACAATGGGCTAATGTCCAACCTCATTCTGGATCATCTGCTAACTATGCAGTTTACTTAGCATTGTTAAAACCAGGAGATACTATCTTAGGATTAGATCTTAATTGTGGTGGTCATTTAACCCATGGTAGCCCCGTTAATTTTTCAGGTAAGCAATATCAAGCAGTAACTTATTCGTTAGATTTTGAAACAGAAACTCTTGATTATGATGCAATTCTTCAAATTGCTCTCGAACACAAACCAAAGTTAATTATTTGTGGTTTTTCTAACTATTCTAGGACTGTTGACTTTAAAAAATTTAGTGCAATTGCAAAACAAGTTAATGCGTATCTTTTAGCTGATATTGCCCATATTGCTGGTTTCATCGCTGCAGGTTTGCACCAAAACCCTTTGCCTTTTGTGGATGTTGTCACTTCAACAACTCATAAAACTTTGCGTGGTCCTAGGGGGGGTATCATTATGTCTAACAACCAAGCAATTATCAAAAAGCTTGATAGTGGAGTATTTCCTGGATGTCAGGGTGGACCTTTACAACATGTGATAGCAGCTAAATATGTTTGTTTTAAAGAAGCTTTGAATCCAAAGTTTAAGCAGTATATGCAACAAGTTAAAGATAATGCTTTAGCAATGGCAAATTGA", "aa": "MFSKVRLLLNKELQRQRENICLIASENYVSQDILAVTGSVLTNKYAEGYPSKRFYQGCEVVDESENLAIESCKTLFGAQWANVQPHSGSSANYAVYLALLKPGDTILGLDLNCGGHLTHGSPVNFSGKQYQAVTYSLDFETETLDYDAILQIALEHKPKLIICGFSNYSRTVDFKKFSAIAKQVNAYLLADIAHIAGFIAAGLHQNPLPFVDVVTSTTHKTLRGPRGGIIMSNNQAIIKKLDSGVFPGCQGGPLQHVIAAKYVCFKEALNPKFKQYMQQVKDNALAMAN", "aa_hexdigest": "bb78f6aee34a47b0e9d9b560a512a0d4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025FA9D98", "query_cov": 0.972318339100346, "subject_cov": 0.8593272171253823, "identity": 0.612, "score": 362.0, "evalue": 1.22e-122, "valid": false, "product": "serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A2E8WS34", "kegg_orthology_id": "K00600" }, "pscc": { "uniref50_id": "UniRef50_A0A2E8WS34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E8WS34" ], "product": "Serine hydroxymethyltransferase" }, "genes": [], "id": "KKNAAMEMLJ_891", "locus": "GCA000027325_00891" }, { "type": "cds", "sequence": "contig_1", "start": 498316, "stop": 499023, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MG395", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47635", "UniRef:UniRef90_P47635" ], "nt": "TTGGGTTATCCTGTACTTGAAAGAGGTTATGATCAAAACCGCTTATTAGAACAAAAAGAAGAGTTTGCTAGAACTCATAACTTTAATCAAAAAAGTCAGTTATGGAGTAAAAACACATATTTAGTTTCAACTGCTAAAGAGATCCCTGTAATTACAAAAAATGCCAGAAAAGATGGTCAAATAGGTAGTGAAATATTTTCCAAAAAAACCCAAGACAGTCATGTTGATAAAGTTATTAAACACGAAAAAGGGATTGTTACATTTCAAAACTTCAAAAACTTTAAACTTAAATATCATGACAAAGAGTACCAACAGTATGGATATGGTTTAATGCTTGATGATACCAATCTCCCTGGTGGTTCTTCTGGAAGTGCTATATTTAACAACAACCAAAAAATTAACAGTATTTATTTTGGTGTTTTAGAGGTATATAAGAATTCCAAAACACATAAAGATAACATTGGTATGTCTCAACTTTTAAGAACTTCTAAACGTGAAAGTGAAAAAAATAAAACCAGAACTACTAATAATAGGGATAAATTTCAGCACTATGATTTAATTTTTGGTGACAGTAACACAAAATCTTTCTATGCACAATTTGCTAAAAAGCACAATACCCACTTGTACGACCAAATTAAAAGTAGTGAAAAGGAAGAATTTAAATATGTTGATAAGAACAATCAAAAAACACCGTTTTTATTACGTTAA", "aa": "MGYPVLERGYDQNRLLEQKEEFARTHNFNQKSQLWSKNTYLVSTAKEIPVITKNARKDGQIGSEIFSKKTQDSHVDKVIKHEKGIVTFQNFKNFKLKYHDKEYQQYGYGLMLDDTNLPGGSSGSAIFNNNQKINSIYFGVLEVYKNSKTHKDNIGMSQLLRTSKRESEKNKTRTTNNRDKFQHYDLIFGDSNTKSFYAQFAKKHNTHLYDQIKSSEKEEFKYVDKNNQKTPFLLR", "aa_hexdigest": "ebf38e281e3d97b819d72760d69bbfb1", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 498316, "stop": 499323, "observations": { "mutation": [ 499044, 499143 ], "stop": [ 499044, 499143 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47635", "query_cov": 1.0, "subject_cov": 0.4484732824427481, "identity": 0.996, "score": 462.0, "evalue": 5.13e-160, "gene_start": 1, "gene_end": 235, "reference_start": 290, "reference_end": 524, "reference_sequence": "MLKIDMWFKLKSLGFSLISLQALLASCSAVSPVPVPIEEKNDSTTDNNATPFKDEQSDQGTEVNQQPKVEQKVYNRNFKFNTSFIPEESDIYRKGYDLTFTLNFTSFSNDSYGTGWLIDWKGDENTTQKQGSFFAYIATNLHVADGLRNIGDHWPYSKTDDQREFNEYESTVYFSIGKYTNKTDITKLYQEEKLEQRKVNDSLLSIQTSNIPKTAYTATNFLKGVNSIKPVYADFAVIELELNLENLRDWQIFNEFIKPAINTYKSLGDSTNIFETKDLEQHWNHSHYLLGYPVLERGYDQNRLLEQKEEFARTHNFNQKSQLWSKNTYLVSTAKEIPVITKNARKDGQIGSEIFSKKTQDSHVDKVIKHEKGIVTFQNFKNFKLKYHDKEYQQYGYGLMLDDTNLPGGSSGSAIFNNNQKINSIYFGVLEVYKNSKTHKDNIGMSQLLRTSKRESEKNKTRTTNNRDKFQHYDLIFGDSNTKSFYAQFAKKHNTHLYDQIKSSEKEEFKYVDKNNQKTPFLLR" }, "paralog": false, "identity": 0.9940298507462687, "score": 671.0, "evalue": 1.15e-244, "description": "Internal stop codon at 499044, 499143. Nonsense mutation around 499044, 499143.", "psc": { "uniref90_id": "UniRef90_P47635", "product": "Uncharacterized lipoprotein MG395", "uniref50_id": "UniRef50_P47635", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47635", "UniRef:UniRef50_P47635", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47635", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47635" ], "product": "Uncharacterized lipoprotein MG395" } }, "id": "KKNAAMEMLJ_892", "locus": "GCA000027325_00892" }, { "type": "cds", "sequence": "contig_1", "start": 499543, "stop": 499890, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAGATTGATATGTGGTTTAAACTAAAAAGTTTAGGTTTTTCACTAATTTCATTACAAGCACTTTTAGCTTCTTGTTCTGCTGTTTCACCTGTTCCTGTTCCTATAGAAGAAAAAAACGACAGCACAACAGATAACAACGCTACTCCTTTTAAAGATGAACAATCTGATCAAGGAACAGAAGTAAATCAGCAACCTAAGGTAGAACAGAAGGTTTACAACAGAAACTTTAAATTTAATACAAGTTTTATCCCAGAAGAAAGTGATATATACAGAAAAGGATATGATTTAACTTTTACTTTAAATTTCACTAGCTTTAGTAATGACTCTTATGGCACTGGTTGA", "aa": "MLKIDMWFKLKSLGFSLISLQALLASCSAVSPVPVPIEEKNDSTTDNNATPFKDEQSDQGTEVNQQPKVEQKVYNRNFKFNTSFIPEESDIYRKGYDLTFTLNFTSFSNDSYGTG", "aa_hexdigest": "073ee48bcc61b1cf34fc5616d8cad6a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13006.326699999987, "isoelectric_point": 4.706632423400878 }, "id": "KKNAAMEMLJ_893", "locus": "GCA000027325_00893" }, { "type": "cds", "sequence": "contig_1", "start": 500051, "stop": 500182, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTAATGAAAGCAAATGCAACCATCAAAAAAGTTAAAAATGAAAATAGAACTACTATTGACTCAGGTAAACTAACTAAATTAAAACCAAAAAAATTAATCCCGTTAGAATTTGCATTGGCTTGA", "aa": "MKKLMKANATIKKVKNENRTTIDSGKLTKLKPKKLIPLEFALA", "aa_hexdigest": "fd1ad18d8bb42f3478c65d5b9d54ca67", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4853.9209, "isoelectric_point": 10.420943641662596 }, "id": "KKNAAMEMLJ_894", "locus": "GCA000027325_00894" }, { "type": "cds", "sequence": "contig_1", "start": 500261, "stop": 500719, "strand": "-", "frame": 2, "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "db_xrefs": [ "COG:COG0698", "COG:G", "EC:5.3.1.6", "GO:0004751", "GO:0009052", "GO:0019316", "RefSeq:WP_009885626.1", "SO:0001217", "UniParc:UPI000013973C", "UniRef:UniRef100_P47636", "UniRef:UniRef50_P47636", "UniRef:UniRef90_P47636" ], "nt": "ATGAGTTTTAATATCTTTATTGCTTCTGATCATACCGGTTTAACTCTTAAAAAAATTATTAGTGAACACCTAAAGACTAAGCAGTTTAATGTAGTTGATCTTGGCCCTAATTATTTTGATGCTAATGATGATTATCCTGACTTTGCTTTTTTGGTAGCTGATAAGGTCAAGAAAAATTCTGATAAAGATCTTGGGATACTAATTTGCGGTACTGGTGTTGGGGTTTGTATGGCAGCAAATAAAGTAAAAGGAGTATTAGCGGCTTTGGTTGTTAGTGAGAAAACAGCAGCTTTAGCTAGACAACATGATAATGCCAATGTTTTATGTCTTTCCAGTCGTTTTGTAACTGACAGTGAAAACATTAAAATAGTGGATGATTTTCTCAAAGCTAATTTTGAAGGTGGTAGACACCAAAGACGGATAGATAAGATTATTCGATATGAAAAAGAAACTGAATAG", "aa": "MSFNIFIASDHTGLTLKKIISEHLKTKQFNVVDLGPNYFDANDDYPDFAFLVADKVKKNSDKDLGILICGTGVGVCMAANKVKGVLAALVVSEKTAALARQHDNANVLCLSSRFVTDSENIKIVDDFLKANFEGGRHQRRIDKIIRYEKETE", "aa_hexdigest": "36c336623729202195316291c6d57640", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013973C", "ncbi_nrp_id": "WP_009885626.1", "uniref100_id": "UniRef100_P47636", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013973C", "RefSeq:WP_009885626.1", "UniRef:UniRef100_P47636" ] }, "ips": { "uniref100_id": "UniRef100_P47636", "uniref90_id": "UniRef90_P47636" }, "psc": { "uniref90_id": "UniRef90_P47636", "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "ec_ids": [ "5.3.1.6" ], "uniref50_id": "UniRef50_P47636", "cog_id": "COG0698", "cog_category": "G", "go_ids": [ "GO:0004751", "GO:0009052", "GO:0019316" ] }, "pscc": { "uniref50_id": "UniRef50_P47636", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47636" ], "product": "Probable ribose-5-phosphate isomerase B" }, "genes": [ "rpiB" ], "id": "KKNAAMEMLJ_895", "locus": "GCA000027325_00895" }, { "type": "cds", "sequence": "contig_1", "start": 500720, "stop": 501487, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG397", "db_xrefs": [ "GO:0000921", "GO:0005940", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47637", "UniRef:UniRef90_P47637" ], "nt": "GTGGTTGAAAATATTGATGATTTTCGTAATCAAATTCAACAATATGAAAAGAAAAATGAGCTTTTAAAAACAACAATTAAAGCCATTGAATTAGTGTTCCAAAATGAAACAGATGTGCATGATTTAATTATTGAGCTTAATGAGAACTGCGATCTAATTACTAAAAATATTAATAACTTAAACCATCAAAGTTTAGCTCAGAATCAAATCAATTATGAAAAACTGTTTTACTTGATGAGTCAAACAAATCAAGCTTTAGAAAAACTGAAAAATTCACTAACTGATTTACTTAATTTAGCAGTTAATAAGTTTGATGACTATCGCTTTTTTGTCTATACTTTAGATGATTTCCGATTTAAATTTTTTCAGATTGAAAATTTAATTAGCGATCAAGATTTAAAGGTTCCTGCTAAAACATTGCAAGTAATTAATAAATCGAAATTAGAACTTGATAACATCTTCACTGAAGCTAATAATAACTATTTAGGTACTTTTAATTTGTTTAGTGAAAAGATAGATTTATTGCAAATTCAATTGACTGAAGTTATTTGTGATGTTGTTAGTTTAGTTAACTTAAGGCTAATGTGCAAACAAGCTTTTCTTTTTGCAAATAAATATCGTCAAAAGAGTAGTCATATCGATAGATCATTAGAACAGTTAACAAAACTATACAATCGACAAAACTATCCTGATACATTAGAAGGATTAATTAATCTTTTAACTAAAATTAAAACCAATACAAAACAAGACTATCTGGAACTGAATTAA", "aa": "MVENIDDFRNQIQQYEKKNELLKTTIKAIELVFQNETDVHDLIIELNENCDLITKNINNLNHQSLAQNQINYEKLFYLMSQTNQALEKLKNSLTDLLNLAVNKFDDYRFFVYTLDDFRFKFFQIENLISDQDLKVPAKTLQVINKSKLELDNIFTEANNNYLGTFNLFSEKIDLLQIQLTEVICDVVSLVNLRLMCKQAFLFANKYRQKSSHIDRSLEQLTKLYNRQNYPDTLEGLINLLTKIKTNTKQDYLELN", "aa_hexdigest": "80047e9a8a58eeb76ee53dffe107920a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 500720, "stop": 501787, "observations": { "mutation": [ 501532 ], "stop": [ 501532 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47637", "query_cov": 1.0, "subject_cov": 0.450530035335689, "identity": 0.996, "score": 488.0, "evalue": 3.15e-169, "gene_start": 1, "gene_end": 255, "reference_start": 312, "reference_end": 566, "reference_sequence": "MLVFLILLPFLLLVIFGAYLLVCLFVRQNNLLLKKTKQLYNNLKLSNFNCVISPFQLLRNEKQALEQSIKVLKQFQIKFDNEINSAFEQLTVYSKPNNIYRFIWFNRNIKLLNKNLQHLYTKQQEYINLTKNAIVYFNNSYDCLVFYRQAFCFLNQFINNFLIEKYDSLFYLNVLNRISLLFKEIENCIQAKDVEKQIAFLNKLHNILSQTIITANRQYMFDIKLSYLTHHFKVLIQKVKQMELMKNKVINSQDLLKLHELINNITIKLKDCTNFLNNLNLMLCERNMDEVQEAISWLFQAVSSKEKSINLVVENIDDFRNQIQQYEKKNELLKTTIKAIELVFQNETDVHDLIIELNENCDLITKNINNLNHQSLAQNQINYEKLFYLMSQTNQALEKLKNSLTDLLNLAVNKFDDYRFFVYTLDDFRFKFFQIENLISDQDLKVPAKTLQVINKSKLELDNIFTEANNNYLGTFNLFSEKIDLLQIQLTEVICDVVSLVNLRLMCKQAFLFANKYRQKSSHIDRSLEQLTKLYNRQNYPDTLEGLINLLTKIKTNTKQDYLELN" }, "paralog": false, "identity": 0.9971830985915493, "score": 693.0, "evalue": 1.72e-252, "description": "Internal stop codon at 501532. Nonsense mutation around 501532.", "psc": { "uniref90_id": "UniRef90_P47637", "product": "Uncharacterized protein MG397", "uniref50_id": "UniRef50_P47637", "go_ids": [ "GO:0016020", "GO:0005940", "GO:0000921" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47637", "UniRef:UniRef50_P47637", "GO:0016020", "GO:0005940", "GO:0000921" ] }, "pscc": { "uniref50_id": "UniRef50_P47637", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47637" ], "product": "Uncharacterized protein MG397" } }, "id": "KKNAAMEMLJ_896", "locus": "GCA000027325_00896" }, { "type": "cds", "sequence": "contig_1", "start": 501530, "stop": 501880, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAGAGATAGAAAATTGTATTCAAGCTAAAGATGTAGAAAAGCAAATTGCTTTTTTAAACAAATTACATAACATTCTCAGTCAAACAATTATTACTGCTAACAGGCAATATATGTTTGATATTAAGTTAAGTTATTTAACCCATCACTTTAAGGTTTTAATTCAAAAGGTAAAACAAATGGAATTAATGAAGAATAAAGTAATTAACAGTCAAGATCTCTTAAAACTTCATGAATTGATTAATAACATTACCATTAAATTAAAGGATTGTACCAATTTTCTTAATAACTTAAATTTAATGCTATGCGAAAGGAATATGGATGAAGTTCAAGAGGCGATTAGTTGA", "aa": "MFKEIENCIQAKDVEKQIAFLNKLHNILSQTIITANRQYMFDIKLSYLTHHFKVLIQKVKQMELMKNKVINSQDLLKLHELINNITIKLKDCTNFLNNLNLMLCERNMDEVQEAIS", "aa_hexdigest": "68b67205dfed8ddc1ff903b3d46ce711", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13736.085999999985, "isoelectric_point": 8.439765739440919 }, "id": "KKNAAMEMLJ_897", "locus": "GCA000027325_00897" }, { "type": "cds", "sequence": "contig_1", "start": 502109, "stop": 502420, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGTTTTTCTAATCTTGCTCCCTTTCTTACTACTAGTAATTTTTGGTGCTTATCTATTAGTTTGTCTTTTTGTTAGACAAAATAACTTACTTTTAAAAAAAACCAAACAACTCTATAATAACTTGAAATTAAGCAATTTCAATTGTGTGATTAGTCCATTTCAATTACTAAGAAATGAAAAACAAGCGTTAGAACAATCCATTAAAGTTTTAAAACAGTTTCAAATCAAATTTGATAATGAAATCAATTCAGCTTTTGAGCAACTAACAGTTTACAGTAAACCAAATAATATTTATCGTTTCATCTGA", "aa": "MLVFLILLPFLLLVIFGAYLLVCLFVRQNNLLLKKTKQLYNNLKLSNFNCVISPFQLLRNEKQALEQSIKVLKQFQIKFDNEINSAFEQLTVYSKPNNIYRFI", "aa_hexdigest": "8ed337e99d3e8149333b4e559996cda4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12188.453599999986, "isoelectric_point": 9.697221565246583 }, "id": "KKNAAMEMLJ_898", "locus": "GCA000027325_00898" }, { "type": "cds", "sequence": "contig_1", "start": 502422, "stop": 502823, "strand": "-", "frame": 1, "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "db_xrefs": [ "COG:C", "COG:COG0355", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0045261", "GO:0046933", "RefSeq:WP_010869467.1", "SO:0001217", "UniParc:UPI00001264F9", "UniRef:UniRef100_P47638", "UniRef:UniRef50_P47638", "UniRef:UniRef90_P47638" ], "nt": "ATGAAGTTATTGCGCTTTTTGGTACTTAGTCCTAGTGGCATAAAACTAGATAAAACCATTATTAGTGCGCAAGTTAAAACTACTGAAGGTTACATAGGATTAAATTTTAATCGCGCTCCTTTGATTGCTGCTATTCAATCCCATCTGTGCAAAATTATTTTTGCTGATCAAACAAAAAGAGAAGCAATTATTGGTGCTGGTTTAATGCTTATTAAAAAAACAGAAGCTAAGATTTTCACAGAAAATTTTGTTTTTGCTGATGAAGTTGATATTAATGAAACCTTAAAAAGAAAAACAGAACTTGAAAGAAAAATTCACCATATCAAGGATGCTAAGCTAAACGTTAAAATTGAACAAAATTTAATGTTTGAACTATTAAAACTTTCAAGTAAGAAAAAATAA", "aa": "MKLLRFLVLSPSGIKLDKTIISAQVKTTEGYIGLNFNRAPLIAAIQSHLCKIIFADQTKREAIIGAGLMLIKKTEAKIFTENFVFADEVDINETLKRKTELERKIHHIKDAKLNVKIEQNLMFELLKLSSKKK", "aa_hexdigest": "9e3ba339b581709bd5a4fab56172c3fa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001264F9", "ncbi_nrp_id": "WP_010869467.1", "uniref100_id": "UniRef100_P47638", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001264F9", "RefSeq:WP_010869467.1", "UniRef:UniRef100_P47638" ] }, "ips": { "uniref100_id": "UniRef100_P47638", "uniref90_id": "UniRef90_P47638" }, "psc": { "uniref90_id": "UniRef90_P47638", "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47638", "cog_id": "COG0355", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045261", "GO:0005524", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47638", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47638" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "KKNAAMEMLJ_899", "locus": "GCA000027325_00899" }, { "type": "cds", "sequence": "contig_1", "start": 502828, "stop": 504258, "strand": "-", "frame": 3, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961", "SO:0001217", "UniParc:UPI000012645F", "UniRef:UniRef100_P47639", "UniRef:UniRef50_P50002", "UniRef:UniRef90_Q50331" ], "nt": "TTGATAAAAAAAGAAAACCTAACATATGGTAAAGTTCACCAAGTCATTGGTCCTGTAGTTGATGTTATCTTTTCAGAAAGTAAACAATTACCTAGAGTTTATGATTGTTTGAGTGTACAACTAAAAAAAAGTGAGCTTTTTTTAGAAGCAACCCAATTAATAGGTGATGACATTGTTCGTTGCATTGCATTAGGTCCTACAGAAGGATTAGCACGTAATGTTAAAGTTACTAACTATAACCATCCAATAGAGGTACCTGTTGGCAAAAATGTATTGGGAAGGATGTTCAATGTTTTAGGTGAACCCATTGATGGAAAAGAACCATTACCAAAAAAACCAAAGCTATCAATCCATCGTAACCCACCTGCTTTTGATGAACAACCAAATACTGTTGATATTTTTGAAACAGGAATAAAAGTAATTGATCTTTTAACTCCTTACGTTAGGGGGGGTAAAATTGGTTTATTTGGAGGAGCTGGTGTTGGGAAAACTGTTTTGGTGCAAGAATTAATTCATAACATTGCCAAAGAACATTCTGGTTTAAGTGTATTTGCTGGAGTTGGTGAAAGAACAAGAGAAGGTAATGATCTTTACTATGAAATGATTCAAGGTGGGGTGATTGATAAAACAGTTTTAGTTTTTGGCCAAATGAATGAACCACCAGGAGCTAGAATGAGAGTTGCTTTAACTGCTTTAACAATGGCAGAATATTTTCGTGATCATGATAATCAGAATGTGCTGTTATTCATTGACAATATTTTTCGTTTTACTCAAGCAGGTAGTGAGGTTTCAGCATTACTTGGTAGAATGCCATCTGCTGTTGGCTATCAACCAACTTTAGCTATTGAAATGGGTAAGTTACAAGAAAGAATTGCTTCTACCAAAACAGGTTCTATTACATCTGTTCAAGCTATCTATGTTCCAGCAGATGATCTAACAGACCCAGCACCTGCAACAACATTTACCCATCTTGATGCTAAAACAGTGTTGGATCGTAATATTGCAGCACTAGGTATTTTTCCAGCAATTAATCCTTTAGAATCAACAAGTCGTTTATTAGATCCTAGTGTTGTTGGTATCAACCATTATAAAGTCGCTTTAGGAGTGCAAAATATCTTGCAGCGTTTTGCAGAATTACAAGATATCATTGCTATACTAGGGATTGATGAATTGTCTGATGAAGATAAGATTATTGTTGAAAGAGCAAGAAGGATACGTAACTTTTTATCCCAACCTTTTTTTGTTGCTGAAAAGTTTTCAGGTATTGCAGGTAAATATGTATCTTTAAATGATACTGTTCAATCTTTTAAAGAAATTTTGGAAGGTAAGCATGATCATTTGCCTGAACAAGCATTCTTTTATGTTGGAACCATTCAAGAGGCTGTTGAAAAAGCAAAAAGATTAAATCAAGAGTTTGATAAAACTAAATAG", "aa": "MIKKENLTYGKVHQVIGPVVDVIFSESKQLPRVYDCLSVQLKKSELFLEATQLIGDDIVRCIALGPTEGLARNVKVTNYNHPIEVPVGKNVLGRMFNVLGEPIDGKEPLPKKPKLSIHRNPPAFDEQPNTVDIFETGIKVIDLLTPYVRGGKIGLFGGAGVGKTVLVQELIHNIAKEHSGLSVFAGVGERTREGNDLYYEMIQGGVIDKTVLVFGQMNEPPGARMRVALTALTMAEYFRDHDNQNVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAIEMGKLQERIASTKTGSITSVQAIYVPADDLTDPAPATTFTHLDAKTVLDRNIAALGIFPAINPLESTSRLLDPSVVGINHYKVALGVQNILQRFAELQDIIAILGIDELSDEDKIIVERARRIRNFLSQPFFVAEKFSGIAGKYVSLNDTVQSFKEILEGKHDHLPEQAFFYVGTIQEAVEKAKRLNQEFDKTK", "aa_hexdigest": "cd9861372bfe463ce716a9f0783997bc", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012645F", "uniref100_id": "UniRef100_P47639", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012645F", "UniRef:UniRef100_P47639" ] }, "ips": { "uniref100_id": "UniRef100_P47639", "uniref90_id": "UniRef90_Q50331" }, "psc": { "uniref90_id": "UniRef90_Q50331", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P50002", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P50002", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P50002" ], "product": "ATP synthase subunit beta, sodium ion specific" }, "genes": [ "atpD" ], "id": "KKNAAMEMLJ_900", "locus": "GCA000027325_00900" }, { "type": "cds", "sequence": "contig_1", "start": 504260, "stop": 504655, "strand": "-", "frame": 2, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_P47640" ], "nt": "ATGGATGCATTTAAAGAGTTTAAACTTGATCGAATTTGTATGGTTTACACTAAATTTAAAAACTCATTAATCCAACAATCTCAGCTCTTTCAAGTTTTTCCTTTCGATGTTGAAACTTTTAAAACTTTAAATCCGGTTGTAACTGATCAACAACTTGATTTTGAGCCAGATCAAGCCACGATAATTAATTTAATTACTCCACAGTTTTTTGATGTGGCTCTGTATGGTGGCCTTGTTGAAACTAAGTTATGTGAATCAGCTTCTAGACAAAATGCAATGGAAGCTGCTACAAAGAATGCTAAAGATTTACTTGATAAATACACTTTACAATTTAACAAGCTAAGACAAAACTCTATTACAGAAGAGATTATTGAAGTTATAGGAGGTATGAATTAA", "aa": "MDAFKEFKLDRICMVYTKFKNSLIQQSQLFQVFPFDVETFKTLNPVVTDQQLDFEPDQATIINLITPQFFDVALYGGLVETKLCESASRQNAMEAATKNAKDLLDKYTLQFNKLRQNSITEEIIEVIGGMN", "aa_hexdigest": "b53748a57bc025a0916f30df669a8818", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "3-prime", "pseudogene": { "start": 504260, "stop": 504955, "observations": { "mutation": [ 504754 ], "stop": [ 504754 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47640", "query_cov": 1.0, "subject_cov": 0.46953405017921146, "identity": 1.0, "score": 259.0, "evalue": 1.23e-85, "gene_start": 1, "gene_end": 131, "reference_start": 149, "reference_end": 279, "reference_sequence": "MAFIQEIKRRMNTVKSTIKITNAMKMVSRAKFIKFKKQFQEISLFFNEFYKAVGQVVVSLKEPKKKPDNQKTLWIMMSSSLGLCGQHNSNMNKLLKANFKADDKIFFLGRKNQSFWNKNSQYNPAVGFIDIQDRDINFDYCQTIFDQIMDAFKEFKLDRICMVYTKFKNSLIQQSQLFQVFPFDVETFKTLNPVVTDQQLDFEPDQATIINLITPQFFDVALYGGLVETKLCESASRQNAMEAATKNAKDLLDKYTLQFNKLRQNSITEEIIEVIGGMN" }, "paralog": false, "identity": 0.9956709956709957, "score": 461.0, "evalue": 1.16e-167, "description": "Internal stop codon at 504754. Nonsense mutation around 504754.", "psc": { "uniref90_id": "UniRef90_P47640", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47640", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "KKNAAMEMLJ_901", "locus": "GCA000027325_00901" }, { "type": "cds", "sequence": "contig_1", "start": 504752, "stop": 505099, "strand": "-", "frame": 2, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_P47640" ], "nt": "ATGGCTTTTATACAAGAAATTAAGCGCAGAATGAATACAGTAAAATCCACCATTAAGATAACTAATGCAATGAAAATGGTGTCACGCGCTAAGTTTATTAAGTTCAAAAAACAGTTTCAAGAAATTAGTTTGTTTTTTAATGAATTTTATAAAGCTGTTGGCCAAGTAGTTGTTTCTTTAAAAGAACCAAAAAAGAAACCAGATAACCAAAAAACTTTATGGATAATGATGAGTTCTTCTTTAGGACTTTGTGGACAGCATAATTCGAACATGAATAAGTTATTAAAAGCTAATTTTAAAGCTGATGATAAAATCTTTTTTTTAGGTAGAAAAAACCAATCATTTTGA", "aa": "MAFIQEIKRRMNTVKSTIKITNAMKMVSRAKFIKFKKQFQEISLFFNEFYKAVGQVVVSLKEPKKKPDNQKTLWIMMSSSLGLCGQHNSNMNKLLKANFKADDKIFFLGRKNQSF", "aa_hexdigest": "8bfd9797ada4917b2a62320d75c157af", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "5-prime", "pseudogene": { "start": 504449, "stop": 505099, "observations": { "mutation": [ 504754 ], "stop": [ 504754 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47640", "query_cov": 1.0, "subject_cov": 0.4121863799283154, "identity": 1.0, "score": 224.0, "evalue": 8.42e-72, "gene_start": 1, "gene_end": 115, "reference_start": 1, "reference_end": 115, "reference_sequence": "MAFIQEIKRRMNTVKSTIKITNAMKMVSRAKFIKFKKQFQEISLFFNEFYKAVGQVVVSLKEPKKKPDNQKTLWIMMSSSLGLCGQHNSNMNKLLKANFKADDKIFFLGRKNQSFWNKNSQYNPAVGFIDIQDRDINFDYCQTIFDQIMDAFKEFKLDRICMVYTKFKNSLIQQSQLFQVFPFDVETFKTLNPVVTDQQLDFEPDQATIINLITPQFFDVALYGGLVETKLCESASRQNAMEAATKNAKDLLDKYTLQFNKLRQNSITEEIIEVIGGMN" }, "paralog": false, "identity": 0.9953703703703703, "score": 430.0, "evalue": 1.33e-155, "description": "Internal stop codon at 504754. Nonsense mutation around 504754.", "psc": { "uniref90_id": "UniRef90_P47640", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47640", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "KKNAAMEMLJ_902", "locus": "GCA000027325_00902" }, { "type": "cds", "sequence": "contig_1", "start": 505099, "stop": 505887, "strand": "-", "frame": 3, "gene": null, "product": "ATP synthase alpha chain", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "nt": "TTGATTGTATTTGATGACCTTTCTAAGCATGCTGTTGCTTACAGAACTCTTTCACTCTTGTTAAAAAGACCACCTGGTAGAGAAGCTTTTCCAGGAGATGTTTTTTATTTACATTCAAGACTTTTGGAACGTGCATGCAAGTTAAATGATGAAAATGGTGGTGGCTCAATTACAGCTTTACCAATTATAGAAACTCAAGCTGGTGATATCTCTGCATATATTCCTACAAATGTTATTTCAATTACTGATGGCCAACTGTTTATGGTTAGTAGTCTATTTAACGCTGGACAACGCCCTGCAATTCAAATTGGTTTATCAGTTTCAAGGGTTGGTAGTGCAGCACAAACAAAAGCGATTAAACAGCAAACTGGCAGTTTAAAACTAGAACTTGCTCAGTATAGTGAACTTGATAGTTTTAGTCAATTTGGTAGTGATCTTGATGAAAATACAAAAAAGGTTTTAGAGCATGGTAAAAGAGTAATGGAAATGATTAAACAACCAAATGGTAAACCTTACTCTCAAGTCCATGAAGCATTATTTTTATTTGCTATTAACAAAGCTTTCATTAAGTTTATTCCAGTTGATGAAATTGCTAAATTTAAACAAAGGATAACAGAAGAATTTAATGGTTCCCATCCTCTGTTTAAAGAGTTATCTAACAAAAAAGAATTTACTGAGGATTTAGAAAGTAAAACTAAAACCGCTTTTAAAATGCTTGTGAAACGTTTTATCAGTACATTAACAGATTATGATATTACCAAATTTGGTAGTATTGAGGAACTTAATTAA", "aa": "MIVFDDLSKHAVAYRTLSLLLKRPPGREAFPGDVFYLHSRLLERACKLNDENGGGSITALPIIETQAGDISAYIPTNVISITDGQLFMVSSLFNAGQRPAIQIGLSVSRVGSAAQTKAIKQQTGSLKLELAQYSELDSFSQFGSDLDENTKKVLEHGKRVMEMIKQPNGKPYSQVHEALFLFAINKAFIKFIPVDEIAKFKQRITEEFNGSHPLFKELSNKKEFTEDLESKTKTAFKMLVKRFISTLTDYDITKFGSIEELN", "aa_hexdigest": "8f4965ef61aad525a43c35b53ea2ecc4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q1RKD9", "query_cov": 0.9351145038167938, "subject_cov": 0.8419243986254296, "identity": 0.58, "score": 279.0, "evalue": 7.85e-91, "valid": false, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q1RKD9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q1RKD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "product": "ATP synthase alpha chain" }, "genes": [], "id": "KKNAAMEMLJ_903", "locus": "GCA000027325_00903" }, { "type": "cds", "sequence": "contig_1", "start": 505909, "stop": 506655, "strand": "-", "frame": 3, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGGCAGATAAACTAAATGAATACGTAGCATTAATCAAAACTGAAATTAAAAAGTATTCCAAAAAAATATTTAACAGTGAAATTGGTCAAGTCATTAGTGTTGCTGATGGAATTGCCAAGGTTAGTGGACTTGAAAATGCTTTATTAAATGAGTTAATTCAATTTGAAAATAATATTCAAGGAATAGTATTAAACCTTGAACAAAATACAGTCGGAATAGCACTTTTTGGTGACTATTCTTCGTTACGAGAAGGCAGTACCGCTAAAAGAACCCACAGTGTAATGAAAACTCCTGTTGGTGATGTTATGCTTGGTAGAATCGTCAATGCACTTGGTGAAGCAATTGATGGTAGAGGTGATATTAAAGCTACTGAATATGATCAAATAGAAAAAATTGCTCCAGGTGTAATGAAAAGGAAAAGTGTTAACCAACCACTTGAAACTGGAATCTTAACAATTGATGCTTTATTTCCTATAGGTAAAGGACAACGTGAATTAATTGTTGGTGATAGACAAACAGGTAAAACTGCTATTGCGATTGACACTATCATTAATCAAAAAGATAAAGATGTTTATTGTGTTTATGTAGCAATTGGTCAAAAAAATTCATCAGTAGCACAAATTGTACACCAACTTGAAGTTAATGATTCAATGAAATACACTACAGTGGTTTGTGCTACAGCTAGTGACTCTGATTCCATGGTTTATTTAAGTCCTTTTACAGGAATAACTATTGCTGAATATTGA", "aa": "MADKLNEYVALIKTEIKKYSKKIFNSEIGQVISVADGIAKVSGLENALLNELIQFENNIQGIVLNLEQNTVGIALFGDYSSLREGSTAKRTHSVMKTPVGDVMLGRIVNALGEAIDGRGDIKATEYDQIEKIAPGVMKRKSVNQPLETGILTIDALFPIGKGQRELIVGDRQTGKTAIAIDTIINQKDKDVYCVYVAIGQKNSSVAQIVHQLEVNDSMKYTTVVCATASDSDSMVYLSPFTGITIAEY", "aa_hexdigest": "f82f3fb9500a29f04daa791e2578ff9f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.9637096774193549, "subject_cov": 0.9372549019607843, "identity": 0.565, "score": 264.0, "evalue": 1.17e-85, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "KKNAAMEMLJ_904", "locus": "GCA000027325_00904" }, { "type": "cds", "sequence": "contig_1", "start": 506671, "stop": 507201, "strand": "-", "frame": 3, "gene": "atpH", "product": "ATP synthase F1 subunit delta", 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"24fcfa4384aa9db8bfb4f51d519b298b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001264A9", "ncbi_nrp_id": "WP_009885621.1", "uniref100_id": "UniRef100_P47642", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001264A9", "RefSeq:WP_009885621.1", "UniRef:UniRef100_P47642" ] }, "ips": { "uniref100_id": "UniRef100_P47642", "uniref90_id": "UniRef90_P47642" }, "psc": { "uniref90_id": "UniRef90_P47642", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47642", "cog_id": "COG0712", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47642", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47642" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "KKNAAMEMLJ_905", "locus": "GCA000027325_00905" }, { "type": "cds", "sequence": "contig_1", "start": 507194, "stop": 507439, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTAAAAAGTGAAATAGAAAAAACAGCAAATCGCCAAGCTAACTTAATGATTTTTCAAGCTCGTCAGGAAATTGAAAAAGAAAGACGTTCTCTTAAAGAACAATCTATTAAAGAGAGTGTGGAATTGGCTATGTTGGCTGCACAAGAACTAATTCTCAAGAAAATAGATCAAAAATCAGATAGAGAATTTATTGATAAGTTTATTAGAGATTTAGAAGCTAACGAAACAGAAGATGATTAA", "aa": "MQLKSEIEKTANRQANLMIFQARQEIEKERRSLKEQSIKESVELAMLAAQELILKKIDQKSDREFIDKFIRDLEANETEDD", "aa_hexdigest": "a8c108098cbf6bdcdc66eb92a6854592", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9596.800399999991, "isoelectric_point": 4.959622764587402 }, "id": "KKNAAMEMLJ_906", "locus": "GCA000027325_00906" }, { "type": "cds", "sequence": "contig_1", "start": 507823, "stop": 508131, "strand": "-", "frame": 3, "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0005886", "GO:0008289", "GO:0015986", "GO:0045263", "GO:0046933", "RefSeq:WP_009885619.1", "SO:0001217", "UniParc:UPI00001265D0", "UniRef:UniRef100_P47644", "UniRef:UniRef50_P47644", "UniRef:UniRef90_P47644" ], "nt": "ATGGAACATGTTAATGAAATTTTAGCTACAGTTGGTGTTATATTACAACAAACTCAAACTACCCAGGATGTTAACGCTAGTGCTAAGCTAGGTGCTTATATAGGTGCTGGTGTTACTATGATTGCAGGTTCAACTGTAGGGATTGGACAAGGTTATATTTTTGGTAAAGCTGTTGAGGCAATAGCAAGAAATCCTGAAGTTGAAAAACAGGTTTTTAAACTAATTTTCATTGGTTCTGCTGTTTCTGAATCTACAGCAATTTATGGACTTTTAATTTCCTTTATCTTAATTTTTGTAGCAGGAGCTTAA", "aa": "MEHVNEILATVGVILQQTQTTQDVNASAKLGAYIGAGVTMIAGSTVGIGQGYIFGKAVEAIARNPEVEKQVFKLIFIGSAVSESTAIYGLLISFILIFVAGA", "aa_hexdigest": "a5fc1b6dc3117211faa233e9c535be74", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001265D0", "ncbi_nrp_id": "WP_009885619.1", "uniref100_id": "UniRef100_P47644", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001265D0", "RefSeq:WP_009885619.1", "UniRef:UniRef100_P47644" ] }, "ips": { "uniref100_id": "UniRef100_P47644", "uniref90_id": "UniRef90_P47644" }, "psc": { "uniref90_id": "UniRef90_P47644", "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47644", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045263", "GO:0008289", "GO:0046933", "GO:0015986" ] }, "pscc": { "uniref50_id": "UniRef50_P47644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47644" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "KKNAAMEMLJ_907", "locus": "GCA000027325_00907" }, { "type": "cds", "sequence": "contig_1", "start": 508134, "stop": 508262, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAGGGTTATGTTTTTGTAATGTTGACTTATAATTATTGGGCTAAAATGCGCAATCAAGGTTTGGAAAATAATAATGCAAGTGAATTACACTTTAAAGGCATAAAGGTAATTCAAGAAAATATTTAG", "aa": "MQGYVFVMLTYNYWAKMRNQGLENNNASELHFKGIKVIQENI", "aa_hexdigest": "c1fd5741c6543a65a1800a45fed40ab2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4993.696999999999, "isoelectric_point": 8.120518302917482 }, "id": "KKNAAMEMLJ_908", "locus": "GCA000027325_00908" }, { "type": "cds", "sequence": "contig_1", "start": 508288, "stop": 508389, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTAATACTGGGGTTATTAAACCTGCAAAAACTGTTCCTAAGCTAAGTGCTAATGGTTGGTTATTAATACTTGAAAAAATAAAAATTCAAAAGTTATAA", "aa": "MCNTGVIKPAKTVPKLSANGWLLILEKIKIQKL", "aa_hexdigest": "59765f25d69fbbb03ccc30b587ed89f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3649.5006000000003, "isoelectric_point": 10.025236701965333 }, "id": "KKNAAMEMLJ_909", "locus": "GCA000027325_00909" }, { "type": "cds", "sequence": "contig_1", "start": 508420, "stop": 508710, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCCCATAACCTTAATGAAATAGAAAAAAGCGGTGCAAATCCACTCAATATACTAAAAGGATTTGGAATGAAAGTAGAATACTTTTTTCCTTTAACAGTAATTCCAAAGGCAAACTCTTTAAAAAAATTTCATCTTTGGTATCTAATCCCCATAACAACAATCCCAATAAAAGTTGCAAGTCCTAAAGAAAAAGTAAATGTTAAAGATGATGTTGGTGGTGAAATACCACCAAGCAAGCTAACTAAGTTGCTTGATACTATGTACAGAAGCAACATTAAAAAGTAG", "aa": "MFPHNLNEIEKSGANPLNILKGFGMKVEYFFPLTVIPKANSLKKFHLWYLIPITTIPIKVASPKEKVNVKDDVGGEIPPSKLTKLLDTMYRSNIKK", "aa_hexdigest": "52e48c4e32d4aa890d5b2d0a5b8d4c0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10895.842099999989, "isoelectric_point": 9.73641834259033 }, "id": "KKNAAMEMLJ_910", "locus": "GCA000027325_00910" }, { "type": "cds", "sequence": "contig_1", "start": 509003, "stop": 509182, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGACATGTTTTCTTTTCGATTTAGATATTAAATTGATAAAAAAATATTCTAAAGGTAACCAAACAAATGATAACACTAAATTTAGTATTGGAAAAAACAATGTTGAAATTATTGTTGCAATAGTGTTAAAAATTGACGGAGTGACAACAAAAGAAATAATCACAGGAATTAAATAA", "aa": "MATCFLFDLDIKLIKKYSKGNQTNDNTKFSIGKNNVEIIVAIVLKIDGVTTKEIITGIK", "aa_hexdigest": "10eba45f6b21e8374251f8065f309968", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6559.688700000001, "isoelectric_point": 9.20764865875244 }, "id": "KKNAAMEMLJ_911", "locus": "GCA000027325_00911" }, { "type": "cds", "sequence": "contig_1", "start": 509198, "stop": 509470, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAAAAATGCAAAGAATGAAACTGCTTTTTGCTTTTTGACATTTTTCAAACTTGCAAAGAAAGATGCTAATCTTAAAACTGATAGTGAATTTAAAACAGCAAAAGGTAATGGCAACATTCAACCAAATAAAATGTCCAAATTATTGCCTTTTGTTAAAACAACAACTGCTATTAAAATTAGTACAAATAACCAAGCTACAAAAATAATTAAGGTAATAATTTTTGCAACCTTTCTATTGTTTCAATTGAACAAAACTTTTAGATGTTAA", "aa": "MKKNAKNETAFCFLTFFKLAKKDANLKTDSEFKTAKGNGNIQPNKMSKLLPFVKTTTAIKISTNNQATKIIKVIIFATFLLFQLNKTFRC", "aa_hexdigest": "ade6e1ebbd4216ea000fa20191d633df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10211.04439999999, "isoelectric_point": 10.232824897766111 }, "id": "KKNAAMEMLJ_912", "locus": "GCA000027325_00912" }, { "type": "cds", "sequence": "contig_1", "start": 509502, "stop": 509876, "strand": "-", "frame": 1, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A925XKF8" ], "nt": "ATGAACCAATTAACTAAAACCATAGGTAGCCATATTCAAATTGTTGGTGATGACACTTACTGTACTAATGCAGAACTTGCTAAAAAAGGTGTTGCACAAAATACAACAAACTCGATATTGATTAAATTAAATCAAATTGGTTCTATTAGTGAAACGATTCAAACAATTGAAGTTGCAAAAAAAGCTAACTGGAGTCAAGTAATTTCACATCGCAGTGGTGAAACAGAAGATACAACTATTGCTGATTTGGCAGTTGCTGCCCAAACTGGTCAAATTAAAACCGGTTCAATGTCACGCTCAGAAAGAATAGCTAAATACAATCGTTTGTTGTACATAGAAATTGAACTTGGTGATAAAGGAAAATACTTAGGTTGA", "aa": "MNQLTKTIGSHIQIVGDDTYCTNAELAKKGVAQNTTNSILIKLNQIGSISETIQTIEVAKKANWSQVISHRSGETEDTTIADLAVAAQTGQIKTGSMSRSERIAKYNRLLYIEIELGDKGKYLG", "aa_hexdigest": "2117d4f4c162a431da0d04778d0fe3dc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1F9NV54", "query_cov": 0.9435483870967742, "subject_cov": 0.9435483870967742, "identity": 0.615, "score": 149.0, "evalue": 4.1e-44, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A925XKF8" }, "pscc": { "uniref50_id": "UniRef50_A0A925XKF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A925XKF8" ], "product": "Enolase" }, "genes": [], "id": "KKNAAMEMLJ_913", "locus": "GCA000027325_00913" }, { "type": "cds", "sequence": "contig_1", "start": 510093, "stop": 510836, "strand": "-", "frame": 1, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2U821" ], "nt": "ATGGGAAGTTCAAATCTAAACATCAATTCAAAAATAACCGATATTTTTGCTTATCAAGTTTTTGATTCTCGGGGTGTTCCAACAGTAGCTTGTGTTGTTAAATTGGCATCTGGTCATGTAGGTGAAGCGATGGTTCCATCAGGTGCTTCTACAGGTGAGAAAGAAGCAATTGAATTACGTGATAATGATCCAAAAAATTATTTTGGTAAAGGCGTTAACGAAGCCGTTGATAACGTTAATAAAGTTATTGCCCCTAAGCTTATTGGCTTAAATGCATTTGATCAATTAACAGTGGATCAAGCAATGATTAAACTAGACAATACTCCCAACAAAGCAAAATTAGGAGCAAATGCTATATTATCTGTTTCACTTGCAGTATCAAAAGCAGCAGCAAAAGCACAAAACAGCTCATTATTTCAATACATTTCAAATAAATTAATTGGATTAAATACAACAAATTTTGTTTTACCTGTGCCAATGTTAAATGTAATTAATGGTGGTGCTCATGCTGATAACTATATTGATTTTCAAGAGTTCATGATCATGCCTTTAGGTGCTAAAAAGATGCATGAAGCTTTAAAAATGGCTAGTGAAACTTTTCATGCTTTACAAAATCTTTTAAAAAAGCGTGGATTAAACACAAATAAAGGAGATGAAGGTGGATTTGCGCCTAACTTAAAACTTGCAGAAGATGCACTTGACATCATGGTTGAAGCCATTAAATTAGCTGGATATAAGCCTTGA", "aa": "MGSSNLNINSKITDIFAYQVFDSRGVPTVACVVKLASGHVGEAMVPSGASTGEKEAIELRDNDPKNYFGKGVNEAVDNVNKVIAPKLIGLNAFDQLTVDQAMIKLDNTPNKAKLGANAILSVSLAVSKAAAKAQNSSLFQYISNKLIGLNTTNFVLPVPMLNVINGGAHADNYIDFQEFMIMPLGAKKMHEALKMASETFHALQNLLKKRGLNTNKGDEGGFAPNLKLAEDALDIMVEAIKLAGYKP", "aa_hexdigest": "78df07f21508be188daa8961fa3017b9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00125F4A82", "query_cov": 0.9595141700404858, "subject_cov": 0.9753086419753086, "identity": 0.5820000000000001, "score": 255.0, "evalue": 2.35e-82, "valid": false, "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_A0A9E2U821", "kegg_orthology_id": "K01689" }, "pscc": { "uniref50_id": "UniRef50_A0A9E2U821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2U821" ], "product": "Enolase" }, "genes": [], "id": "KKNAAMEMLJ_914", "locus": "GCA000027325_00914" }, { "type": "cds", "sequence": "contig_1", "start": 511373, "stop": 512050, "strand": "-", "frame": 2, "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "db_xrefs": [ "COG:COG0704", "COG:P", "GO:0006817", "GO:0030643", "GO:0045936", "RefSeq:WP_009885614.1", "SO:0001217", "UniParc:UPI0000139775", "UniRef:UniRef100_P47649", "UniRef:UniRef50_P47649", "UniRef:UniRef90_P47649" ], "nt": "ATGGAAAACATTAATTACCAAATTTTAAAGCGTTCAGAAAAGAAATTATTAAAGCTTTTTTTTGAGTATTTTAAGCACGTAATCAATGCACATGAAACATTAAATCAATTACTTTGTGAAGATAATTTAGAAAAACGCAAAGAACTGATTAAAACTATTTATGAAATGGAGGACCAATCTAATAGGTCTGAATTTAAATTAATTAATGAATCAATTTGGACAATCTCTAAAAATTCTCCATTAGCTAGCCATTTACGCCTAACAATCACAATAATTATGTCAAGTCGTGACTTGGAACGCATTTGTGATTATGCTAATAATTTAACTAAGTTTATAGAACATTATTTGCATCTTGATATAAAGATTTTTAATAATCTAGTTACTTTGCATCAAAGTGCGCTCAATAACCTTAAGAAAACCTTTGAAAGTTTACAAGATAAAGAAAAGCCACTTACATTACAATTTGAAAATGCAACCAAAACTATTATTGAATTTGAGCACCAATATCGCCTTGTATTAACAAAATACTATGAAACCATTAATAAAAATGAGGTTACTGAAAGGATTTTCTTAATTGATCTTGTAGCTTCTGTTAAACATATTGAACGGATTAATGATTATTGCTATAACATCATTAAATCCTTTTTATTTATTAAAAATCCTGAAATATTTAATTAG", "aa": "MENINYQILKRSEKKLLKLFFEYFKHVINAHETLNQLLCEDNLEKRKELIKTIYEMEDQSNRSEFKLINESIWTISKNSPLASHLRLTITIIMSSRDLERICDYANNLTKFIEHYLHLDIKIFNNLVTLHQSALNNLKKTFESLQDKEKPLTLQFENATKTIIEFEHQYRLVLTKYYETINKNEVTERIFLIDLVASVKHIERINDYCYNIIKSFLFIKNPEIFN", "aa_hexdigest": "7d23fa54e12868ad1047f677b660b29f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139775", "ncbi_nrp_id": "WP_009885614.1", "uniref100_id": "UniRef100_P47649", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139775", "RefSeq:WP_009885614.1", "UniRef:UniRef100_P47649" ] }, "ips": { "uniref100_id": "UniRef100_P47649", "uniref90_id": "UniRef90_P47649" }, "psc": { "uniref90_id": "UniRef90_P47649", "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "uniref50_id": "UniRef50_P47649", "cog_id": "COG0704", "cog_category": "P", "go_ids": [ "GO:0006817", "GO:0030643", "GO:0045936" ] }, "pscc": { "uniref50_id": "UniRef50_P47649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47649" ], "product": "Uncharacterized protein MG409" }, "genes": [ "phoU" ], "id": "KKNAAMEMLJ_915", "locus": "GCA000027325_00915" }, { "type": "cds", "sequence": "contig_1", "start": 512053, "stop": 512397, "strand": "-", "frame": 3, "gene": "pstB", "product": "Phosphate import ATP-binding protein PstB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H5ZIP6" ], "nt": "GTGAAAGATAATTTACATAGGAATGCAAACACCCTTTCTGGTGGACAACAACAACGCTTGTGTATTGCGCGTGCTATTGCTTTACAACCAGATGTTCTTTTGATGGATGAACCTACCAGTGCTTTAGACTCAATTGCCACAAACTCTATTGAACTTCTAATTCAACAACTAAAAGAAAAATTCACAATTGTTATTGTTACTCACTCTATGGCTCAAACAATTAGAATAACTGATGAAACGATTTTTTTTGCTGATGGAAGAGTAATTGAACAAGGCACTACAAAACAGATATTTACAAAGCCTAAGCAAAAAGCAACAAATAGTTATATAAGTGGGAAAAATTAG", "aa": "MKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKFTIVIVTHSMAQTIRITDETIFFADGRVIEQGTTKQIFTKPKQKATNSYISGKN", "aa_hexdigest": "27bc3ce0688b10abfcd82c5b5794130f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2H5ZIP6", "query_cov": 0.9912280701754386, "subject_cov": 0.9826086956521739, "identity": 0.628, "score": 135.0, "evalue": 6.34e-39, "valid": false, "product": "Phosphate import ATP-binding protein PstB", "uniref50_id": "UniRef50_A0A2H5ZIP6" }, "pscc": { "uniref50_id": "UniRef50_A0A2H5ZIP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H5ZIP6" ], "product": "Phosphate import ATP-binding protein PstB" }, "genes": [ "pstB" ], "id": "KKNAAMEMLJ_916", "locus": "GCA000027325_00916" }, { "type": "cds", "sequence": "contig_1", "start": 512404, "stop": 512559, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCACTAGTGTTGGCATGGTTTTTCAAAAATTAACTCCTTTTAATTTTTCTATTTTTGAAAACATTGCTTATGGCATAAGAGCACATGGTATTCACAATAAAAATGCTATCAATGAAATAGTAAGACAGGCATTGATATCAGCAGCATTGTGA", "aa": "MRTSVGMVFQKLTPFNFSIFENIAYGIRAHGIHNKNAINEIVRQALISAAL", "aa_hexdigest": "73a271350e22243bf8d8a872801ce725", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5703.6006, "isoelectric_point": 10.266477394104005 }, "id": "KKNAAMEMLJ_917", "locus": "GCA000027325_00917" }, { "type": "cds", "sequence": "contig_1", "start": 512770, "stop": 513003, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGCTTGAAAAAATTAAGCATTACCGAAAGCTTTATGAACAACAAATCAAAGAATATAAAAAGAAAATTACTGGTTTAAATAATGAAACAGATGCAAATGAAATCTCCCGTATTAAGAATGAAATTGAAATTTTAAACCGTCTAATAAAGATTAAAAACACCAAAGATAATGTCATTAAAAAGGATTTTGATGAAAAAAATGTATTTGAAATTCGAAATTTCAACTTCTGA", "aa": "MKLEKIKHYRKLYEQQIKEYKKKITGLNNETDANEISRIKNEIEILNRLIKIKNTKDNVIKKDFDEKNVFEIRNFNF", "aa_hexdigest": "50beeb59b346aa4761fdbccacd785efb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9458.854499999992, "isoelectric_point": 9.583563804626461 }, "id": "KKNAAMEMLJ_918", "locus": "GCA000027325_00918" }, { "type": "cds", "sequence": "contig_1", "start": 513027, "stop": 513656, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTAAAAATGTTTTTAACTTTGTAATTGATTCACTAAGTTCAATGCCATCTATTATTTATGGATTATTTGGACTTTCTTTCTTTTTAAGAGTCTTGCAGTTAAGTGCTGGAGGAGCTAATGGTACTAGTTTAATAGCAGGCATTCTAACTATTAGTGTTGTTATATTACTCTTCCTTATAAGAACTTGTCAACAAGCACTAAATAATGTCAGTTGGGATTTAAGAATTAGTGCTTTTGCTTTAGGTATAAGTAAACGTGAAGTTATTTTCAAAATAGTTTTACCTAGTGCTTTGAAAGGATTAATAGTTGCATTAATTTTGTCAATCAACAGAATTATTGCTGAAACTGCACCCTTCTTTATCACTTCAGGGTTATCATCTAGTAATTTATTTCATTTGTCATTGCCAGGTCAAACACTAACAACAAGGATATATGGACAGTTATTTTCTATTAATAGCAATGCAATAAGTGTTATGTTAGAAACATCATTGGTCTCTGTTGTTTTCTTAATTCTTTTAATCTTTTTCAGTTCTTATTTAATCCCGAGTTTATTTTTGTTAAATAAACAAAAATGGCTAGTAATTAAAAGTAAATTTCAGTCCTTTAAATTATGGAAAAGAACATAA", "aa": "MVKNVFNFVIDSLSSMPSIIYGLFGLSFFLRVLQLSAGGANGTSLIAGILTISVVILLFLIRTCQQALNNVSWDLRISAFALGISKREVIFKIVLPSALKGLIVALILSINRIIAETAPFFITSGLSSSNLFHLSLPGQTLTTRIYGQLFSINSNAISVMLETSLVSVVFLILLIFFSSYLIPSLFLLNKQKWLVIKSKFQSFKLWKRT", "aa_hexdigest": "da7a747a1cc06c8a8bee03c732f05d80", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23113.41250000001, "isoelectric_point": 10.48998928070068 }, "id": "KKNAAMEMLJ_919", "locus": "GCA000027325_00919" }, { "type": "cds", "sequence": "contig_1", "start": 513681, "stop": 513857, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGCAATTTTGCTGTTTGTATTTATTAATGGAAGTGTTGCTATTAATAATAATGGTTCTACTGTTTTTTCATTTGAAGCTGATTCAACTGGCAGAGCACTAGTAAATACTCTAGTAATTATTTTGATTACTATCACCATTACTTTTCCACTAGCACTTTTAATTGCAATTTGA", "aa": "MLAILLFVFINGSVAINNNGSTVFSFEADSTGRALVNTLVIILITITITFPLALLIAI", "aa_hexdigest": "4b00b38fded486895dad3aca19e75e35", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6151.298899999996, "isoelectric_point": 4.369065666198731 }, "id": "KKNAAMEMLJ_920", "locus": "GCA000027325_00920" }, { "type": "cds", "sequence": "contig_1", "start": 514131, "stop": 514781, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAGTTTTATTGTATCAATAGGAGCTTTAATTATTGCTAGTTATATAGGGGTTAGAACTTCATTTTTCCTTGTTTATCGATGCAAACCAAAAATAAGAAAAAAACTTTCACTTATTATTGATATCCTTTCAGGAATACCATCTGTAATTTTTGGATTATTTGCATCACAAATATTAAGCATTTTCTTTCGGGATATCTTGAAATTACCGCCGCTTTCACTTTTAAATGTGATAGCTATGCTTTCTTTTATGATCATTCCTATTGTTATTTCATTAACAACAAATACATTAACTTATGTAAATAACGATCTAATTAGTGTTGTTGTTTCCTTAGGGGAAAATAAAACAAGTGCGATCTACAAAATTATTAAAAAAGAAATTAAACCACAATTAACAGTTATTTTGACCTTAGCCTTTGCGAGAGCAATTAGTGAAACAATGGCTGTTAACTTTGTTTTGCAGAGTGTTAACTATCAAGAGGTAATTAACAACAATCGTTTTTTTACTTCTGATTTAAAAACACTGGGATCAGTTATTTCCACTTTTATTTTTTCAGAAAATGGAGATGAACAGATTAATGGTGTTTTATATATCTTTGGAATCATAATTTTGATATTAGTTTCATTGTTAAATTTCTTTGCCATTTGA", "aa": "MVSFIVSIGALIIASYIGVRTSFFLVYRCKPKIRKKLSLIIDILSGIPSVIFGLFASQILSIFFRDILKLPPLSLLNVIAMLSFMIIPIVISLTTNTLTYVNNDLISVVVSLGENKTSAIYKIIKKEIKPQLTVILTLAFARAISETMAVNFVLQSVNYQEVINNNRFFTSDLKTLGSVISTFIFSENGDEQINGVLYIFGIIILILVSLLNFFAI", "aa_hexdigest": "fc853dcdcdd492bec880a4c58a73b54a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24009.50100000001, "isoelectric_point": 9.518257331848144 }, "id": "KKNAAMEMLJ_921", "locus": "GCA000027325_00921" }, { "type": "cds", "sequence": "contig_1", "start": 514791, "stop": 514991, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAAGAAGATTAAAAAACGTTTAAAAAAAGAGAATCTTTTAAGAATCTTTTCAAAGACTTTAGCATTCTTGTTTTTAGTTTTATTTATAAGTTTTTTTGTTTTTCTTTTAACAGAAGCAACAAAAATTGGACCTGATTTTGCAAAGTCCTTGTTTAATCTTGAATTTAATTTAGGTAATAAACAGGCAGGAATTTGA", "aa": "MQKKIKKRLKKENLLRIFSKTLAFLFLVLFISFFVFLLTEATKIGPDFAKSLFNLEFNLGNKQAGI", "aa_hexdigest": "7bb897ee20a5264b8e15e2030168972f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7669.207399999995, "isoelectric_point": 10.358860588073728 }, "id": "KKNAAMEMLJ_922", "locus": "GCA000027325_00922" }, { "type": "cds", "sequence": "contig_1", "start": 515249, "stop": 515635, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATGAAAAAGATTACCCTTTAACAAGTTTTCCAAGAACTGGTGGGTCATTTGCTTCAGGAACAGCAGAAGCTTTTTTAAATTTTTCCGCCTTAAAATCAGATAAAACTTTAGATTCTCAAACTAGAGATATTTTGAAAGGAATTATTAATTATGGACCTCTTGCTAAACCTACTAGTGAAACAAATATTGAAGCTTTTAATACTTTTGTAACTAACTTACAAGATCCTAATCTTTTCGGGATGATTTACCTTAGTTTAGGATTTATTCAAAACAACCTTCAAGCTATTAAAAATAATGGTTTTGAGATACTACCAATTAAATATGAGAATAAGGAAGTTTTGCCTTCTAATCAAACTGTTTCCCAAAATGAGTATAAATGA", "aa": "MSNEKDYPLTSFPRTGGSFASGTAEAFLNFSALKSDKTLDSQTRDILKGIINYGPLAKPTSETNIEAFNTFVTNLQDPNLFGMIYLSLGFIQNNLQAIKNNGFEILPIKYENKEVLPSNQTVSQNEYK", "aa_hexdigest": "de5df9895df02f71adb9041ae27c8b70", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14250.831799999985, "isoelectric_point": 5.070288276672362 }, "id": "KKNAAMEMLJ_923", "locus": "GCA000027325_00923" }, { "type": "cds", "sequence": "contig_1", "start": 515819, "stop": 516124, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAAAGTTTAGAAATTTCTTTAAATTGACGCTACTAACATTAGCATCTGCTTTTTTTCTTAGTGGTTGTGCTAACATAAACCTGATTAGTGCTGTTGGTTCTTCTTCAGTTCAACCTTTACTAAATAAATTAAGTTCTTATTATGTTCTAAATGAAAATAATGACAATGATAAGCTTGTTGAAATTAGTGTGCAAGCAGGCGGATCAAATGCTGGAATCAGAGCAATCATTAACGGCTTTGCTGATATCGGAAATGTCTCTAAAAACCCTAAGGAATATGCAAAAGAGAATGAAAAAAAATGA", "aa": "MLKFRNFFKLTLLTLASAFFLSGCANINLISAVGSSSVQPLLNKLSSYYVLNENNDNDKLVEISVQAGGSNAGIRAIINGFADIGNVSKNPKEYAKENEKK", "aa_hexdigest": "7a1afa657b5932eb86f633012480eda9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10959.39219999999, "isoelectric_point": 9.131189155578614 }, "id": "KKNAAMEMLJ_924", "locus": "GCA000027325_00924" }, { "type": "cds", "sequence": "contig_1", "start": 516527, "stop": 517270, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTCATTATCAAAAATAGTTTTAAATCTAAGGATAAGAATATTGATTTAGATATCAACAGTAACTCTTTAACATTTGTGGATAACCCTAAAAAATATTTCAAACATCTAGAGCCTACTAATGAAAAGAAAGGTATATTTTATGTTTTAGCAGAAATAAATAATAGCAATGAACTATTCAAATATGGTTCTGAAAGTGATTCTGAAATTATTAAAGACAAAATGTATTTTCTATCACAAAATCAAAAAAACCCTAAATTAAGAACATATTTATTTAAATTTCATACTAACAAACTTTTTGTTGATAAAAATGACCTGGGATTTAAGTTAGAAAAAGACAAGGTATTTTTATCAGTTGATAACTTAAAAATTGCTGAATTAGATTTAAAGTCATCTTCATTTAAATTTCTAGAGGATTATCAACTTGATTTTCATGAACCTTTTAGTTTAAATGACGAACAATTACTAGTTGATAAACTCAATATTACTCTAAGTGAAAAAAGATTGCAAACAACTAAAAACGTCCGCTTTAACCTAAAGAATAAATTTATTAACATCCATTTAGTAGAAAATAAAAATCAATTTAATTTAGTTTTTGATGTCGATGTTAGATCAAAAAAATTATTTATTAAAGGTGTTAATAATGATAACCAAGTATTTAGTATTAGCTATGATTTAAAAATAACAAATAATCAAACACTTTTAATTGTAGATGCCAATGGTTTTGATAATTCTATTTGA", "aa": "MAFIIKNSFKSKDKNIDLDINSNSLTFVDNPKKYFKHLEPTNEKKGIFYVLAEINNSNELFKYGSESDSEIIKDKMYFLSQNQKNPKLRTYLFKFHTNKLFVDKNDLGFKLEKDKVFLSVDNLKIAELDLKSSSFKFLEDYQLDFHEPFSLNDEQLLVDKLNITLSEKRLQTTKNVRFNLKNKFINIHLVENKNQFNLVFDVDVRSKKLFIKGVNNDNQVFSISYDLKITNNQTLLIVDANGFDNSI", "aa_hexdigest": "d93d3864bc9fca0b3ae220163c7b82e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28982.705900000044, "isoelectric_point": 8.726843452453611 }, "id": "KKNAAMEMLJ_925", "locus": "GCA000027325_00925" }, { "type": "cds", "sequence": "contig_1", "start": 517376, "stop": 518014, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTGATATTAATTCGCTTGTTAGTCAAACTGAAAAAACTCTAAATAAAGAAACAATAGGTACAAAAATTAAACCAACTATTAAATTTAAAGATAAATTCATAAATGCAATTAAAGAAGTTAGTTTAATTAATCAAAGAATTGATGAATCAATTGATAAAAATGAAGCGTTAAAAAGCTTTAATATTTCAACCAATAATCAAAGTAATTTTTATCCAAATTTAGATTATTTATACAACCTATTGCAAATGTCACCAAACAATAAAGAAGAACTATTCTTTATTAGAAATTTACCAAGAATGGTCAAAACCATTTTTGATAGATCAACATTAACTGTTAAAGTAAAAATTGGTAATTCTGTAAATGAAATAACATTGTTAAGAAATAACAAGAATTATTTTGATTTAAGTTCAATTGAGGAGTTTTTAACAGAAAAACAGAAAGCAGATAATGAAATGAATATTGAATTTTTAGCACTTAATTTTTTTGTAGATGGATATGAATCAGAAAATATTAGTAACTATAGTGTTGAACCACTGTTTGACAGTATTAAAAAACTTTCAACTATCAAAAGAACTAAGGATGGTTTTGAATACAAATTTAAATATAGAAAAGATTTTAATGAACAGCGTTGA", "aa": "MEFDINSLVSQTEKTLNKETIGTKIKPTIKFKDKFINAIKEVSLINQRIDESIDKNEALKSFNISTNNQSNFYPNLDYLYNLLQMSPNNKEELFFIRNLPRMVKTIFDRSTLTVKVKIGNSVNEITLLRNNKNYFDLSSIEEFLTEKQKADNEMNIEFLALNFFVDGYESENISNYSVEPLFDSIKKLSTIKRTKDGFEYKFKYRKDFNEQR", "aa_hexdigest": "9df99f3453272cb1153c648f073701fe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25017.10970000002, "isoelectric_point": 7.727454185485839 }, "id": "KKNAAMEMLJ_926", "locus": "GCA000027325_00926" }, { "type": "cds", "sequence": "contig_1", "start": 518024, "stop": 518317, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTATAAATGAGTTTAAAGAGAAAATGAAAAAGGATAGAAAAATCATTGAAGCATTTACTAATAATCGTGAAATAACTCAAACGATATTAAGACTAGGTAATGGATTAGTAGTTAGACCAGAAACTGAAGATAGTAGACGTGAAGCATTCAACAGTAAATGGGATATTTTTAAACATACACCTATATTACCTGAACAAGAAGTTGTTCATCATCAAATAAGTTTTTATCTAAGGCAAAATAATCCTGATGAAGTTTTACCAATTAGTTTCAGTAATCTTTCAAAGCTTTGA", "aa": "MSINEFKEKMKKDRKIIEAFTNNREITQTILRLGNGLVVRPETEDSRREAFNSKWDIFKHTPILPEQEVVHHQISFYLRQNNPDEVLPISFSNLSKL", "aa_hexdigest": "877648ccd427e491af86a706198662e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11495.98579999999, "isoelectric_point": 8.070232963562013 }, "id": "KKNAAMEMLJ_927", "locus": "GCA000027325_00927" }, { "type": "cds", "sequence": "contig_1", "start": 519104, "stop": 519355, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTCATATCTTTTTATTCCTTTATTAGTTTCTTTTCTTTTTCCCGTTGCTTTAGCTAATGCTTCACTTGAAAAAAACTTGGTAGATACTAATCTTTATCTCTCTCCAAACACAATTCAAAACAGAAGCTATACAGCTGGATTTAATTTAAAAAACGATTATTTTCGAAAAAGCTTTAATTTTGATAATGAAGTAATTCAAAGACCTAATCAAAATAATTATTTTGCAAAAACTTATTCGAATGAATGA", "aa": "MRSYLFIPLLVSFLFPVALANASLEKNLVDTNLYLSPNTIQNRSYTAGFNLKNDYFRKSFNFDNEVIQRPNQNNYFAKTYSNE", "aa_hexdigest": "e0c9180b16a3e8b9dd28cf214476f950", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9725.829599999994, "isoelectric_point": 9.047960472106933 }, "id": "KKNAAMEMLJ_928", "locus": "GCA000027325_00928" }, { "type": "cds", "sequence": "contig_1", "start": 519468, "stop": 519962, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002EE4522", "UniRef:UniRef90_UPI0002EE4522" ], "nt": "ATGAATCTTTTAATAGATAAAAAGAAATCTAGCTTAGATCTTTTTCCTAAAGAGATCAAATTAACTACTCTAAAGATAAATAGTTCGCTTCATTTTTATTTAAATGTTGATGCAATTAAAAATCAGCTCAATATTGAAGTTAATATAAGTAAAAATAGATTAACTAGCCTTGTTTATGATATTGCTATCAAAAATGAAAATGAATTGCAAATTAGAACAACAAATAACTACTTAAATAAATATATTTGGTTTGATCTTGATAAGAAAAATAATCAGAAACTGAAAAATGAGCTTAAGTTATTTCTCTCATTAAAAAAATTTCAATTTAAAAAAGAACCTAATTTTAGCTTAAAGAAAAACAGCTATAGTTTTCAAATAGACAAAATTATTCAATCTAATAGTGAAGATAAAAAAACAGATATTATAGTATATTTGATTATTGGTTTTAGTGTTTTAGTTTTGTTTATCACTGTTTTTATTTACTTTCATAAGTGA", "aa": "MNLLIDKKKSSLDLFPKEIKLTTLKINSSLHFYLNVDAIKNQLNIEVNISKNRLTSLVYDIAIKNENELQIRTTNNYLNKYIWFDLDKKNNQKLKNELKLFLSLKKFQFKKEPNFSLKKNSYSFQIDKIIQSNSEDKKTDIIVYLIIGFSVLVLFITVFIYFHK", "aa_hexdigest": "2f74aa835235b30e1b57e012b9b89a87", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002EE4522", "query_cov": 1.0, "subject_cov": 0.9162011173184358, "identity": 1.0, "score": 303.0, "evalue": 7.19e-104, "valid": true, "uniref50_id": "UniRef50_UPI0002EE4522" }, "pscc": { "uniref50_id": "UniRef50_UPI0002EE4522", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002EE4522" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19476.589199999995, "isoelectric_point": 9.657186698913574 }, "id": "KKNAAMEMLJ_929", "locus": "GCA000027325_00929" }, { "type": "cds", "sequence": "contig_1", "start": 520140, "stop": 520640, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTCTATTCAGAAAACTAAAAATGGTTTTCAATATAGTTTAAAGTTTAGATCTGAATACAATGAACATCATATTTTAAAAGATCATTTTTTAATTCCTATTGTTACAAATCAGAAAAATTTCAGTGTTAATGACATTGAAAAAAACGGATTAAATAGTTATCAAAGAGAACAAATTACATATGCAATTAAAAATAGTTTTACTTCTCAAAAAGAAAACAATTTAAATATTTCATCCGCTACAATAAAATACATAAAAGATCCTGAGAAATTAATTAAAAAATCTTTAATTAAGCCCAGTGTTAAAAATGGTATATTCTATGTTTCAGCACAAATAATTAACAGTAATGATTTAACTAAATGGGGTTCTAAAAATGATAGCGAAATCATTAAAGATAAGATGTATTTCTTAGAACAAAATAAAAACTTTCCTGCAATAAGAACTTATCTTTTTCAAATGAGAACCAAAAAGTTAGTTTTAAATGTTAATGATATTTGA", "aa": "MPSIQKTKNGFQYSLKFRSEYNEHHILKDHFLIPIVTNQKNFSVNDIEKNGLNSYQREQITYAIKNSFTSQKENNLNISSATIKYIKDPEKLIKKSLIKPSVKNGIFYVSAQIINSNDLTKWGSKNDSEIIKDKMYFLEQNKNFPAIRTYLFQMRTKKLVLNVNDI", "aa_hexdigest": "1789ac8c6b497b4f70dc97ab62b91294", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19459.105399999997, "isoelectric_point": 9.697866249084473 }, "id": "KKNAAMEMLJ_930", "locus": "GCA000027325_00930" }, { "type": "cds", "sequence": "contig_1", "start": 520689, "stop": 521333, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F2F2D0", "UniRef:UniRef90_UPI0002F2F2D0" ], "nt": "TTGATTGATACTAATCTTTATCTAGTTAAAGATGATTTTCAATCACAAAATCAATTAACTATAGCAACAAATCAACTTGCTAAGATCATTGTTAACCAAATTGAATTTGATTCAAATTCTTTAATAGCTAATCCAACTACTGTTTTAAACAAAGAACTAATAGGATCAAAAATAACACCAAAGCTGAAATTTAGTGATCAATTTTCTAATGCAATTGAAATGGTAAGTAAGCTAAATCAAGAGTTTGATCAATTAGCAAATAAAGATAAAACTTTTTTTCAATTTGCTTTAGATTTATTAGAAAAACAGGAAGAATCTAAATTTGATTTTGAACCAAAAGATGAAAGAATTGATGCAATATTTTTCTTAAGTAATTTAATTAACATTAATCCAAAACAAGAGAAAACATTAAATTTTATTCGGATATTACCCAACCTAATTAAAAGTATTTTTAAAGATACTACCATCACGATAAATATAAAGATAGGTGGCAAAAATAAAGTAATTACTTTTATAGAAAATGGCAGTAACGTTTTTTTATTAAGTGATGTTGAAAATTTCTTAAATGCTGATCAAACTGGTATTAATTTTTATGAAATTGAGTTTTTAACTTTTGATTTTATTGTTGTGAATAAAACAGGATGA", "aa": "MIDTNLYLVKDDFQSQNQLTIATNQLAKIIVNQIEFDSNSLIANPTTVLNKELIGSKITPKLKFSDQFSNAIEMVSKLNQEFDQLANKDKTFFQFALDLLEKQEESKFDFEPKDERIDAIFFLSNLININPKQEKTLNFIRILPNLIKSIFKDTTITINIKIGGKNKVITFIENGSNVFLLSDVENFLNADQTGINFYEIEFLTFDFIVVNKTG", "aa_hexdigest": "da89400994f088c781a1f598fc9493ea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 520386, "stop": 521414, "observations": { "mutation": [ 520691, 521390, 521408 ], "stop": [ 520691, 521390, 521408 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002F2F2D0", "query_cov": 1.0, "subject_cov": 0.4562899786780384, "identity": 0.995, "score": 407.0, "evalue": 8.9e-140, "gene_start": 1, "gene_end": 214, "reference_start": 28, "reference_end": 241, "reference_sequence": "MSWKRYLKWVSFAIIPLLFANTSIKSKLIDTNLYLVKDDFQSQNQLTIATNQLAKIIVNQIEFDSNSLIANPTTVLNKELIGSKITPKLKFSDQFSNAIEMVSKLNQEFDQLANKDKTFFQFALDLLEKQEESKFDFEPKDERIDAIFFLSNLININPKQEKTLNFIRILPNLIKSIFKDTTITINIKIGGKNKVITFIENGSNVFLLSDVENFLNADQTGINFYEIEFLTFDFIVVNKTGWTLKNQPVDSFFKSVKNLPSIQKTKNGFQYSLKFRSEYNEHHILKDHFLIPIVTNQKNFSVNDIEKNGLNSYQREQITYAIKNSFTSQKENNLNISSATIKYIKDPEKLIKKSLIKPSVKNGIFYVSAQIINSNDLTKWGSKNDSEIIKDKMYFLEQNKNFPAIRTYLFQMRTKKLVLNVNDIWFKSSGDKLRVIVNNVEIDEFNPKENNTSIFWKLWSSYKWLFFFS" }, "paralog": false, "identity": 0.9912280701754386, "score": 656.0, "evalue": 2.92e-240, "description": "Internal stop codon at 520691, 521390, 521408. Nonsense mutation around 520691, 521390, 521408.", "psc": { "uniref90_id": "UniRef90_UPI0002F2F2D0", "uniref50_id": "UniRef50_UPI0002F2F2D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002F2F2D0", "UniRef:UniRef50_UPI0002F2F2D0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002F2F2D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F2F2D0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 24550.77500000003, "isoelectric_point": 4.768586921691894 }, "id": "KKNAAMEMLJ_931", "locus": "GCA000027325_00931" }, { "type": "cds", "sequence": "contig_1", "start": 521470, "stop": 521868, "strand": "-", "frame": 3, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627", "KEGG:K02996", "RefSeq:WP_010869475.1", "SO:0001217", "UniParc:UPI00001351B7", "UniRef:UniRef100_P47656", "UniRef:UniRef50_P31782", "UniRef:UniRef90_P47656" ], "nt": "ATGGATAAAAAATCTTTTTATGGACTTGGTCGTCGTAAATCTTCTACTGCTAAAGTTTATTTATATCAAAGCAAAGATAAGGGTAAAATAACCATTAATCATCGTAATCCTAGCGATTATTTTCCAAATAAATTGGTGATTCAAGATATGGAACAACCCTTAGAGTTAACCAAACTTAAGGATAACTTTGATATCAATGTTGTTGTTAAAGGTGGAGGATTTACTGGGCAGGCAGGAGCCATTAGATTAGGTATTGTTCGTGCTTTAATAAAATTTAATCCAGATCTTAAAAAGTTATTAAAAACCAAAAAATTAACAACACGTGATAAGCGTGCTAAGGAACGCAAAAAATTTGGTTTATATGGTGCTAGACGTGCACCACAATTTACTAAGCGTTAG", "aa": "MDKKSFYGLGRRKSSTAKVYLYQSKDKGKITINHRNPSDYFPNKLVIQDMEQPLELTKLKDNFDINVVVKGGGFTGQAGAIRLGIVRALIKFNPDLKKLLKTKKLTTRDKRAKERKKFGLYGARRAPQFTKR", "aa_hexdigest": "094428f8d3565fd2dfcbc16c293a5f25", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001351B7", "ncbi_nrp_id": "WP_010869475.1", "uniref100_id": "UniRef100_P47656", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001351B7", "RefSeq:WP_010869475.1", "UniRef:UniRef100_P47656" ] }, "ips": { "uniref100_id": "UniRef100_P47656", "uniref90_id": "UniRef90_P47656" }, "psc": { "uniref90_id": "UniRef90_P47656", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_P31782", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P31782", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P31782" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "KKNAAMEMLJ_932", "locus": "GCA000027325_00932" }, { "type": "cds", "sequence": "contig_1", "start": 521880, "stop": 522065, "strand": "-", "frame": 1, "gene": "rplM", "product": "50S ribosomal protein L13", "db_xrefs": [ "COG:COG0102", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625", "KEGG:K02871", "SO:0001217", "UniRef:UniRef50_P47657", "UniRef:UniRef90_P47657" ], "nt": "ATGGGTGGAATTAAAAAAACTAGTGGAAGGGATATGATAAACAAAAATTCAGATAAACTTGTTTTCAATGCTGTTAAGGGAATGTTACCTGATAATCGTTTAAGCAGAAGATTAATAACTAAAGTACATGTTTTTAAGAATGATAAGCACAACATGGAAGCACAAAAACCAACATTACTTAATTGA", "aa": "MGGIKKTSGRDMINKNSDKLVFNAVKGMLPDNRLSRRLITKVHVFKNDKHNMEAQKPTLLN", "aa_hexdigest": "d8458e5909285375f3e86844599ca9a5", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplM" ], "truncated": "3-prime", "pseudogene": { "start": 521880, "stop": 522314, "observations": { "mutation": [ 522086 ], "stop": [ 522086 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47657", "query_cov": 1.0, "subject_cov": 0.4178082191780822, "identity": 1.0, "score": 122.0, "evalue": 1.36e-34, "gene_start": 1, "gene_end": 61, "reference_start": 84, "reference_end": 144, "reference_sequence": "MQKTSMLTKEEAIKNRKWYLVDASGLVLGKLAVKAANLIRGKNKANFTPNQDCGDHLIIINSDQVVLTGNKKDNEFWYHHSQYMGGIKKTSGRDMINKNSDKLVFNAVKGMLPDNRLSRRLITKVHVFKNDKHNMEAQKPTLLNWS" }, "paralog": false, "identity": 0.9930555555555556, "score": 289.0, "evalue": 1.11e-102, "description": "Internal stop codon at 522086. Nonsense mutation around 522086.", "psc": { "uniref90_id": "UniRef90_P47657", "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_P47657", "cog_id": "COG0102", "cog_category": "J", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47657", "UniRef:UniRef50_P47657", "COG:COG0102", "COG:J", "KEGG:K02871", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47657" ], "product": "Large ribosomal subunit protein uL13" } }, "id": "KKNAAMEMLJ_933", "locus": "GCA000027325_00933" }, { "type": "cds", "sequence": "contig_1", "start": 522084, "stop": 522314, "strand": "-", "frame": 1, "gene": null, "product": "50S ribosomal protein L13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025A24A7C" ], "nt": "ATGCAAAAAACATCGATGCTTACAAAGGAAGAAGCCATTAAAAACAGGAAGTGGTATCTTGTTGACGCTAGTGGTTTGGTTTTAGGCAAATTAGCAGTTAAAGCTGCAAATTTAATTAGAGGAAAAAATAAAGCTAATTTTACTCCTAATCAAGATTGTGGAGATCATCTAATAATTATTAACAGTGATCAAGTGGTTTTAACTGGAAATAAAAAAGACAATGAGTTTTGA", "aa": "MQKTSMLTKEEAIKNRKWYLVDASGLVLGKLAVKAANLIRGKNKANFTPNQDCGDHLIIINSDQVVLTGNKKDNEF", "aa_hexdigest": "cca961f7519060653a5ca6c9aeb7432b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025A24A7C", "query_cov": 0.9342105263157895, "subject_cov": 0.8658536585365854, "identity": 0.535, "score": 84.7, "evalue": 4.78e-20, "valid": false, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_UPI0025A24A7C", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0025A24A7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025A24A7C" ], "product": "50S ribosomal protein L13" }, "genes": [], "id": "KKNAAMEMLJ_934", "locus": "GCA000027325_00934" }, { "type": "cds", "sequence": "contig_1", "start": 522352, "stop": 522468, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAACTTAACCCAATTGGAAAACTTAGCTAAGCCTTTTTTAAAAGAAAAGAAACGCTCTTTATCACAAAAAATGGTGGATAAATACTTTAAAGGACTTTTTGAAGAAAAGTAA", "aa": "MLNLTQLENLAKPFLKEKKRSLSQKMVDKYFKGLFEEK", "aa_hexdigest": "c693fa80aa9868ed8366ba16f4a65cf7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4574.410099999999, "isoelectric_point": 9.698317527770996 }, "id": "KKNAAMEMLJ_935", "locus": "GCA000027325_00935" }, { "type": "cds", "sequence": "contig_1", "start": 522811, "stop": 523917, "strand": "-", "frame": 3, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_P47658", "UniRef:UniRef90_P47658" ], "nt": "GTGTGTAAAAGTATTAACACTAATAGTGCCATTGATATAGTTGAGATAGATGCAGCTTCTAAAAATGGTATTAATGATATTAGAGAGTTGGTAGAAAATGTTTTCAATCATCCCTTCACATTTAAAAAAAAGGTTTATATTTTAGATGAAGCACACATGTTAACCACCCAATCATGGGGTGGCTTGTTAAAAACTTTAGAAGAATCACCACCTTATGTTCTTTTTATTTTTACAACTACTGAATTTAACAAGATTCCATTAACAATTTTGTCCAGATGTCAAAGCTTCTTTTTTAAAAAAATAACTAGTGATTTAATCCTTGAAAGATTAAATGATATAGCAAAAAAAGAAAAGATTAAGATAGAGAAAGATGCATTAATAAAAATTGCTGATTTATCCCAAGGTTCATTGCGTGATGGGCTTAGCTTACTAGATCAAATTAGCAATTTCTCTGATAGTGAAAAAATTAGTATTACTGATGTTGAAAAAACATTTAATATCGTTGATAGAAATGCAAAATTTACTTTTATTAAAGCAGTTTTATCAGGAGATATAAAAGAAGCATTTAACTTGCTTGATGATTTTGAAAGTAATGGTCTAAATTTCACTTACTTCTTAAGAGAGTTGTTTGCGCTAACAGTTAATTTATATGCGTATGCAAAACTAAAAAATATTAATGTTTTAGATTCAACTGAAAAAACAATGATAGAAACACTTAATTTTGAAAAGCAGCATTATGCGTTTTTAATTAAGGCCATTGAAGAAAATACTAATTTTGGTTTAAGTCAATTAACTTTAATTGATAGGCTAAAAGCTATTGTTATTAGCTATAATGAATTTTTTAATCAAAAACCTTTAACAATAAGCTCACCAAGTAATGAAAAATCTCTACACTTAGAAACTGAATATTTGGAAAAGAAAAAAATAAAAAAATCAAATCACAAACAAGACCAAAAGCACTTTTCTTTATTTGAAAAATCTTTTATAGATAAATCAGAAAAGACACCTAAAAATGATGAAGTTACTAATAACAAATTCTTAGATACAAGCAAACTAAATCTTGCCAATATTGCTCTTGCAATTAATGCATTTAATGACAATAAATGA", "aa": "MCKSINTNSAIDIVEIDAASKNGINDIRELVENVFNHPFTFKKKVYILDEAHMLTTQSWGGLLKTLEESPPYVLFIFTTTEFNKIPLTILSRCQSFFFKKITSDLILERLNDIAKKEKIKIEKDALIKIADLSQGSLRDGLSLLDQISNFSDSEKISITDVEKTFNIVDRNAKFTFIKAVLSGDIKEAFNLLDDFESNGLNFTYFLRELFALTVNLYAYAKLKNINVLDSTEKTMIETLNFEKQHYAFLIKAIEENTNFGLSQLTLIDRLKAIVISYNEFFNQKPLTISSPSNEKSLHLETEYLEKKKIKKSNHKQDQKHFSLFEKSFIDKSEKTPKNDEVTNNKFLDTSKLNLANIALAINAFNDNK", "aa_hexdigest": "ea6d560d97375f74a2b53a47aee34959", "start_type": "GTG", "rbs_motif": null, "genes": [ "dnaX" ], "truncated": "both", "pseudogene": { "start": 522508, "stop": 524145, "observations": { "mutation": [ 522612, 522813, 523950 ], "stop": [ 522612, 522813, 523950 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47658", "query_cov": 1.0, "subject_cov": 0.6164154103852596, "identity": 0.997, "score": 697.0, "evalue": 1.4e-249, "gene_start": 1, "gene_end": 368, "reference_start": 77, "reference_end": 444, "reference_sequence": "MHQVFYQKYRPINFKQTLGQESIRKILVNAINRDKLPNGYIFSGERGTGKTTFAKIIAKAINCLNWDQIDVCNSCDVCKSINTNSAIDIVEIDAASKNGINDIRELVENVFNHPFTFKKKVYILDEAHMLTTQSWGGLLKTLEESPPYVLFIFTTTEFNKIPLTILSRCQSFFFKKITSDLILERLNDIAKKEKIKIEKDALIKIADLSQGSLRDGLSLLDQISNFSDSEKISITDVEKTFNIVDRNAKFTFIKAVLSGDIKEAFNLLDDFESNGLNFTYFLRELFALTVNLYAYAKLKNINVLDSTEKTMIETLNFEKQHYAFLIKAIEENTNFGLSQLTLIDRLKAIVISYNEFFNQKPLTISSPSNEKSLHLETEYLEKKKIKKSNHKQDQKHFSLFEKSFIDKSEKTPKNDEVTNNKFLDTSKLNLANIALAINAFNDNKWINHFQNLLSVFQTKFNDKDKQNNLSYFNNFIDKYSARDIVKATKIVKASSFGIVILFEDQKIAMRLWKEAIEEGNVQATIFQIFNQNLFLASFSEHQYKTTITEETKNQKYQTEVLNLTQLENLAKPFLKEKKRSLSQKMVDKYFKGLFEEK" }, "paralog": false, "identity": 0.9944954128440368, "score": 1058.0, "evalue": 0.0, "description": "Internal stop codon at 522612, 522813, 523950. Nonsense mutation around 522612, 522813, 523950.", "psc": { "uniref90_id": "UniRef90_P47658", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47658", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47658", "EC:2.7.7.7", "UniRef:UniRef50_P47658", "COG:COG2812", "COG:L", "KEGG:K02343", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47658", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47658" ], "product": "DNA polymerase III subunit gamma/tau" } }, "id": "KKNAAMEMLJ_936", "locus": "GCA000027325_00936" }, { "type": "cds", "sequence": "contig_1", "start": 523948, "stop": 524145, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF13177.12" ], "nt": "ATGCACCAAGTTTTTTATCAAAAATATCGGCCAATCAATTTCAAACAAACCCTAGGACAAGAATCGATAAGAAAAATCTTGGTGAATGCTATTAACAGGGATAAACTACCTAATGGTTATATCTTTTCAGGTGAAAGAGGAACAGGTAAAACTACTTTTGCAAAGATAATAGCAAAAGCGATAAACTGCTTAAATTGA", "aa": "MHQVFYQKYRPINFKQTLGQESIRKILVNAINRDKLPNGYIFSGERGTGKTTFAKIIAKAINCLN", "aa_hexdigest": "d3470f6a56b9b116dda894a5ae7cc9fc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028A06DEE", "query_cov": 0.9538461538461539, "subject_cov": 0.96875, "identity": 0.5, "score": 69.3, "evalue": 2.39e-14, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A7V9ZZE4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 22, "aa_cov": 0.3384615384615385, "hmm_cov": 1.0, "evalue": 7.308952306327042e-06, "score": 21.570632934570312, "start": 41, "stop": 62 }, { "id": "PF13177.12", "name": "DNA_pol3_delta2", "length": 46, "aa_cov": 0.7076923076923077, "hmm_cov": 1.0, "evalue": 1.763813390402356e-09, "score": 32.901039123535156, "start": 19, "stop": 64 } ], "seq_stats": { "molecular_weight": 7458.626899999999, "isoelectric_point": 10.176801872253417 }, "id": "KKNAAMEMLJ_937", "locus": "GCA000027325_00937" }, { "type": "cds", "sequence": "contig_1", "start": 524151, "stop": 525785, "strand": "-", "frame": 1, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "nt": "ATGTCTGAAATAACATGTAAAAAGTGTCATGGAAAAAAATTAATAAAAGACGCTTTAAGTGTTAAGTTAGGAGGAATTGACATTATTAGCTTTACTGAACTTTCCATTGATAAAAGTATTGATTTTCTATTAAAACTAGAGTTAAATGATGAGCAAAAGAAGATCGGTGAATTAGCTTTAAAAGAGATTATTAATCGTCTTTCTTTTCTTAAAAATGTTGGTTTAGATTATCTTAATCTTGCAAGAAGAGCTTCTACGCTGTCAGGTGGAGAAGCACAAAGAATTAGATTAGCTACCCAAATTGGTTCTCAACTTACTGGTGTTTTATATGTAATGGATGAACCTTCTATTGGATTGCATCAAAAAGACAATATGCGTTTAATTAAAACAATGATGGTAATGCGTGATTTAGGTAACACCTTATTAGTAGTTGAGCATGACAGTGAAACAATGTTAGCGGCAGATTATTTAATTGATATTGGTCCTAAAGCAGGTAATGAAGGTGGTGAATTAGTTGCTTGCGGTACACCTTTACAAGTAATGGAAAACTCAAACTCAATTACTGGACAATATCTTAGTGGTAAAAAACAAATCTCCATTCCAAAAAATAGACATAGTGGTAATGGTAAAACAATTATTATCAAGGGTGCTAAAGTTAATAATTTGAAAAATATTAATGTCACCATTCCTTTAAATAAATTGGTTTTGATAACAGGGGTTTCAGGTTCTGGAAAATCCTCTTTAATTAATCAAACATTAGTTCCAGCTTTAGAAAGAATTCTTTATCGTAAAGGTGTTAAAAAAGATACATATAAGGAAATAATTGGTGCTAACAACATTGATAAGATAATTGTTGTCTCTCAAGACCCAATTGGTAGAACACCACGTTCTAATCCTGCAACCTATATTAGTGTTTTTGATGATATTCGTGATTTATTTGCCAACACAAAAGAAGCTAAAGCAAGAGGATATACAAATTCACGATTTTCTTTTAATGTTCCAGGTGGTAGGTGTGATAAGTGTTTTGGTGATGGTGTGATTCGCATTGAAATGCATTTTTTACCTGATGTTTATGTCAAATGTGAAGTATGTAATGGCAAGAAGTACAATTCACAAACACTGGAAATTAAATATTTGGGAAAATCAATTTTTGATGTTTTACAAATGTCTTGTAAAGAAGCTTATGAATTTTTTAAAGCTATCCCAAATATATCACGTAAACTAAGGTTGTTATGTGATGTTGGTTTAGAATATTTGCAATTAGGTATTAATGTCACTTTTCTTTCAGGTGGGGAAGCACAGAGAATTAAGTTATCTAAGTTTTTACAAAAAAAATCTACTGGTAAAACTTTGTTTGTTTTAGATGAACCCTCTACTGGCTTACATTTAGAAGATATAAACAAACTATTAACAATAATTCAAAGAATCATTAAGAATGGTGATACAGTAGTTGTTATAGAACATAACTTAGATATTATTAAGGTTGCTGACTATATCATTGATTTAGGTCCTGAAGGTGGTGACAATGGTGGTCAAATTGTTGCTCAAGGAACACCTGAACAACTTATAAACCAAGTTAATAAATCTTATACTGCCCAATATTTGTCCAAAATTTTAAAACCAGATTCAATTTAA", "aa": "MSEITCKKCHGKKLIKDALSVKLGGIDIISFTELSIDKSIDFLLKLELNDEQKKIGELALKEIINRLSFLKNVGLDYLNLARRASTLSGGEAQRIRLATQIGSQLTGVLYVMDEPSIGLHQKDNMRLIKTMMVMRDLGNTLLVVEHDSETMLAADYLIDIGPKAGNEGGELVACGTPLQVMENSNSITGQYLSGKKQISIPKNRHSGNGKTIIIKGAKVNNLKNINVTIPLNKLVLITGVSGSGKSSLINQTLVPALERILYRKGVKKDTYKEIIGANNIDKIIVVSQDPIGRTPRSNPATYISVFDDIRDLFANTKEAKARGYTNSRFSFNVPGGRCDKCFGDGVIRIEMHFLPDVYVKCEVCNGKKYNSQTLEIKYLGKSIFDVLQMSCKEAYEFFKAIPNISRKLRLLCDVGLEYLQLGINVTFLSGGEAQRIKLSKFLQKKSTGKTLFVLDEPSTGLHLEDINKLLTIIQRIIKNGDTVVVIEHNLDIIKVADYIIDLGPEGGDNGGQIVAQGTPEQLINQVNKSYTAQYLSKILKPDSI", "aa_hexdigest": "7fc3ae9816ba064af80282cdda0ced0c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGE6", "query_cov": 0.9301470588235294, "subject_cov": 0.9980276134122288, "identity": 0.6859999999999999, "score": 709.0, "evalue": 1.47e-252, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0PGE6", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PGE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "product": "UvrABC system protein A" }, "genes": [], "id": "KKNAAMEMLJ_938", "locus": "GCA000027325_00938" }, { "type": "cds", "sequence": "contig_1", "start": 526029, "stop": 526547, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17760.6" ], "nt": "GTGCAATTATTAGCACCTACTGTTAAAAATCAGCGCGGCATTTTTACAAATGAATTTATTAAATACAAGCAATTAGGTTTTCTTAGAGTCTTAGTTGATGGCCAGATTTACACCTTAGATGATGAAATTAAACTTGATAAAAATACTAAACACAACATTAGTGTAGTGATCGATAGAATTATCATCAATAAAGATAATCAAACTTATTCAAGGATAGTTGATAGCATTGAAACCATTGATAGGTTAACTAATGGCAAGATAGAAGTTCTTAAGGAAGATGGAACAATATTAAATTTCAGCAAAAATCATGGTTGTGATAAATGTGGTTTTTCTATTAGTGAATTGGAACCAAGATTATTTTCCTTTAACTCCCCTTTAGGTTCATGTTCATATTGCAAAGGACTTGGTTTTAGTTATGAACCTGATGTAGACAAGATAATTGCTGATTCTAAACTTTCTATTAACCAAGGAGCCATTGATATTTTTAAAAATATTGTGCATGGAACTTCTTTGGATTGA", "aa": "MQLLAPTVKNQRGIFTNEFIKYKQLGFLRVLVDGQIYTLDDEIKLDKNTKHNISVVIDRIIINKDNQTYSRIVDSIETIDRLTNGKIEVLKEDGTILNFSKNHGCDKCGFSISELEPRLFSFNSPLGSCSYCKGLGFSYEPDVDKIIADSKLSINQGAIDIFKNIVHGTSLD", "aa_hexdigest": "b8f3495ab2700b05fcf00e9b121af830", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17760.6", "name": "UvrA_inter", "length": 92, "aa_cov": 0.5348837209302325, "hmm_cov": 0.9782608695652174, "evalue": 6.64332932900444e-29, "score": 95.11275482177734, "start": 1, "stop": 92 } ], "seq_stats": { "molecular_weight": 19364.896699999994, "isoelectric_point": 5.9281023025512685 }, "id": "KKNAAMEMLJ_939", "locus": "GCA000027325_00939" }, { "type": "cds", "sequence": "contig_1", "start": 526987, "stop": 527250, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTAGTGATTTAAACAAAATTAACAAAAATGAAAGTCTAAAGCTTTACTTGCTATTTAAACTTTTGTTAAATCCTAAGTTACTAATGATAAACTGCTGCAATGATTTCACTAACCACGCTTATAATTTCATTCGTGGACTTTTGATCTCATATCAAAATAAACAAGGTATTGCGCTTATTTTCAATGATCCTAATAATAAGTTAGTTAAAAATTTTTCAATTAAAAACATTGACTTTGAAACCGGAATGAAAAAATAA", "aa": "MLFSDLNKINKNESLKLYLLFKLLLNPKLLMINCCNDFTNHAYNFIRGLLISYQNKQGIALIFNDPNNKLVKNFSIKNIDFETGMKK", "aa_hexdigest": "429b3469fa69df6b62b9c4719dd5368c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10172.93089999999, "isoelectric_point": 9.517290306091308 }, "id": "KKNAAMEMLJ_940", "locus": "GCA000027325_00940" }, { "type": "cds", "sequence": "contig_1", "start": 527251, "stop": 527520, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGATATTGTATTTTAAACAAAACATTGCACTTAAGACTTAAAAAAACAAACAAGAAAATTAAGAGAACTTTTTTTATTGATTCAGAAGAGTGCAATATCAATCGTTTAGAAGCTTCAAATAAGCTTTATCTTAATGTTTTGAATTCACAATTAAAAACAATTAATTTCTTTTTAAGATCACAAAAAAATCTTAAAAAACTTAACTTTATTAACAACATTAATTTATTGATAAATCAAACCAAAAAGAATGGTATTAGCAGCTGA", "aa": "MKRYCILNKTLHLRLKKTNKKIKRTFFIDSEECNINRLEASNKLYLNVLNSQLKTINFFLRSQKNLKKLNFINNINLLINQTKKNGISS", "aa_hexdigest": "ccc4e5b3dee370ba2bf23391e0954ee2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10586.416799999986, "isoelectric_point": 10.466264915466308 }, "id": "KKNAAMEMLJ_941", "locus": "GCA000027325_00941" }, { "type": "cds", "sequence": "contig_1", "start": 527971, "stop": 528324, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGCACGTTTCATTCTAACGAAAAAAGAGGTTCATGAATTTTTGATCAAAGCACGTTTATCTTTTATTAAAAATTTTGTTAAACAATCAAACAATTTGTATGTTCATGAAAAATTGGTTAATGAACTAAAAGAAAACATCTTATTAAATCAAAATCATTATAGAGGTAAGCATAATAACAGTTTTTCAGTTCTAATCCAAAAAACAGTTTTCAAACAGTTTATTGATACAGTGCAATATGCAATTGGTTTAATCAGCATGAGAAAAAGTTTGGAGACTTTTAACTTTGCATTTCAAATAAAGCAATATTTTTATGAAAGCTTATTAGCTAATTATCAAAAAATTAACTGA", "aa": "MSARFILTKKEVHEFLIKARLSFIKNFVKQSNNLYVHEKLVNELKENILLNQNHYRGKHNNSFSVLIQKTVFKQFIDTVQYAIGLISMRKSLETFNFAFQIKQYFYESLLANYQKIN", "aa_hexdigest": "eb02fa39a72aecf1f5b43725808ba7c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13998.116299999987, "isoelectric_point": 9.859166145324707 }, "id": "KKNAAMEMLJ_942", "locus": "GCA000027325_00942" }, { "type": "cds", "sequence": "contig_1", "start": 528358, "stop": 528510, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAATTAAGAAAATGGATTTACTTTCTCCAAAATTACTTGTAGAAACTATTGATGAAAGTATTAATTTAGTCAATAAAATCTTTGCTAATATTCAAAATCTTTATCAAGAAATTATCAGCTTAAAATCAGGTCCTAATTTGAACTGA", "aa": "MKEIKKMDLLSPKLLVETIDESINLVNKIFANIQNLYQEIISLKSGPNLN", "aa_hexdigest": "285690f0b19ed777462c4dd447e6441b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5714.691599999999, "isoelectric_point": 6.090263938903808 }, "id": "KKNAAMEMLJ_943", "locus": "GCA000027325_00943" }, { "type": "cds", "sequence": "contig_1", "start": 528604, "stop": 529041, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAATTGAAAATGTTAATCAAAAAACTAGTAAAGTCATTAATCAGTTTTTTACATCAAAATTTATTAATTCAATCTACTTTGAACAAGTTGATTTTGAATACAATAATTTTTTAACTTTAATTGAAAAAAACGAAAGAGAACTTGTTGATTTTAATTGCATGTTTTTCAATCAATTATTTACTTTTAATAACAAAGTAATAAAAAGTTTTTTTGAGCGTTACTATGCATTGAACGAACAAAAAAATAACTTAAAAAACAAACTGAAAATAGCTGCTTATGAAGCTAAATCACGAGATAACAGTTATTTTGAAAAATTGCAAATTGAAAGAGCTAAACAAAGACTTTTTGATGCTAAGAAAACATTCTCAAAACAACATAAAAATGCTTTGCAATTAATTAATTCATTCACCTTTTCTTTAATTTTTAGTGGTTGA", "aa": "MKIENVNQKTSKVINQFFTSKFINSIYFEQVDFEYNNFLTLIEKNERELVDFNCMFFNQLFTFNNKVIKSFFERYYALNEQKNNLKNKLKIAAYEAKSRDNSYFEKLQIERAKQRLFDAKKTFSKQHKNALQLINSFTFSLIFSG", "aa_hexdigest": "c9c279d5abd121adf29633adf5310fc5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17488.78919999999, "isoelectric_point": 9.542755317687988 }, "id": "KKNAAMEMLJ_944", "locus": "GCA000027325_00944" }, { "type": "cds", "sequence": "contig_1", "start": 529087, "stop": 529278, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGTTAATAACCATCAATATTTACCGCTTCAAAAAATTAAGTTTAATTTATGTGAAGAGATAGATTTAAGAAATAAAAATAAGTGGTTATTTCCTAATTCATTTAGCATTTTTCTAAAACAAGATTTAAGTTATAAAAAAGATCAAAATTACTTTAATTTAGTGAATATACCTAAACAGTTAAAGTGA", "aa": "MLVNNHQYLPLQKIKFNLCEEIDLRNKNKWLFPNSFSIFLKQDLSYKKDQNYFNLVNIPKQLK", "aa_hexdigest": "47b12d9341e17987ac73c366ebaa765d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7716.933899999998, "isoelectric_point": 9.56325626373291 }, "id": "KKNAAMEMLJ_945", "locus": "GCA000027325_00945" }, { "type": "cds", "sequence": "contig_1", "start": 529330, "stop": 529494, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAGAAAATATCTCTTTTTGATAATCTAGAACAGTGGGTTGATTTGTCATTATTAATGATTCGTACCTCCTTTTTAGATGCTAACTATTTTGGGGTTGCAAAGCTATCATTATTTAAGTATGGTAACTATCTCATTGATAATTTAAATCAAGCACAATGA", "aa": "MLKKISLFDNLEQWVDLSLLMIRTSFLDANYFGVAKLSLFKYGNYLIDNLNQAQ", "aa_hexdigest": "bb841f550bd964534f6c153e74b74ce0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6317.288900000001, "isoelectric_point": 5.955214500427247 }, "id": "KKNAAMEMLJ_946", "locus": "GCA000027325_00946" }, { "type": "cds", "sequence": "contig_1", "start": 529532, "stop": 530977, "strand": "-", "frame": 2, "gene": "rnjA", "product": "putative protein MG423", "db_xrefs": [ "COG:COG0595", "COG:J", "GO:0003723", "GO:0005886", "GO:0046872", "RefSeq:WP_234943733.1", "SO:0001217", "UniParc:UPI0000557C32", "UniRef:UniRef100_UPI0000557C32", "UniRef:UniRef50_P47662", "UniRef:UniRef90_P47662" ], "nt": "TTGTTCCATACAGTAGGATTTTTTCCTATTTACACAAGTTCAATAGGCGCTTCTATAATTAAAAGCAAAATTAATGAAAACAAACTTAATATTGCACGTGATAAATTGGAAATTCATGAATTAAAACCACTTGAAACAATCGAAATTTCTAATCATTCTATTACCCCTTTTAAAGTATCAAGTTCTTTACCATCATCATTTGGATTTGCTTTAAATACTGATAATGGTTACATCGTTTTTATTGATGATTTCATTGTTTTAAATGATAAGAACATTGCTTTTGAAAATCAACTAAATCAAATTATCCCTAAACTTTCTGACAATACATTGTTATTAATAACAGGAGTTGGTTTAGTTGGTAGAAACAGTGGTTTTACAACACCTAAACACAAGTCATTAGAACAGTTAAACAGGATCATAACTCCAGCCAAAGGCAGAATATTTGTAGCTTGTTATGATTCAAATGCTTACAGTGTTATGACTCTTGCCCAGATTGCAAGGATGCAAAATCGTCCTTTTATTATCTATTCACAATCATTTGTGCATCTCTTTAACACAATTGTGCGTCAAAAATTGTTTAATAACACGCATTTAAATACCATTTCTATTGAGGAGATTAACAATTCAACCAACAGTATTGTTGTTCTCACCTCACCACCTGATAAGTTGTATGCTAAATTATTCAAAATTGGAATGAACGAAGATGAAAGAATTCGTTATCGTAAGAGTGATACTTTTATCTTTATGACTCCTAAAGTTGCTGGCTATGAAGAGATAGAAGCACAAATCCTTGATGATATTGCACGTAATGAAGTTAGTTATTACAACTTAGGTAGAGAAATCCTTTCTATCCAAGCAAGTGATGAAGATATGAAGTTTTTAGTTAGTTCTTTAAAACCAAAATATATTATTCCAACTGGTGGACTTTATAGGGATTTTATAAACTTCACAATGGTTTTAAAACAAGCTGGCGCTGAGCAAAATCAAATCTTAATTCTTTTTAACGGTGAAGTTTTAACTATTGAAAACAAAAAACTAGATAGCAAAAAAAATGAATTGAAATTAAATCCTAAGTGTGTTGATTCTGCTGGATTACAAGAAATTGGAGCTAGCATTATGTTTGAACGTGATCAAATGTCAGAATCTGGAGTTGTGATCATAATCATTTATTTTGATCAAAAAAAGAGTGAATTCTTAAATGAGATAACTTATTCTTTTTTAGGAGTATCTTTAGATGTACCAGAAAAAGATAAACTAAAAACAAAAATGGAAGAATTAATTAAAAAACAAATTAATGATATTAAGGATTTCACCACGATTAAAAAACGAATAGGTAAAGAAATTTCAAAAGAATTGAAGGTTTCTATTAAACGAGCAGTAATGAATTTATTCACCAAGATGACATCAAAAGCACCCTTAATTCTTTCAACTATTATTTCTATTTAA", "aa": "MFHTVGFFPIYTSSIGASIIKSKINENKLNIARDKLEIHELKPLETIEISNHSITPFKVSSSLPSSFGFALNTDNGYIVFIDDFIVLNDKNIAFENQLNQIIPKLSDNTLLLITGVGLVGRNSGFTTPKHKSLEQLNRIITPAKGRIFVACYDSNAYSVMTLAQIARMQNRPFIIYSQSFVHLFNTIVRQKLFNNTHLNTISIEEINNSTNSIVVLTSPPDKLYAKLFKIGMNEDERIRYRKSDTFIFMTPKVAGYEEIEAQILDDIARNEVSYYNLGREILSIQASDEDMKFLVSSLKPKYIIPTGGLYRDFINFTMVLKQAGAEQNQILILFNGEVLTIENKKLDSKKNELKLNPKCVDSAGLQEIGASIMFERDQMSESGVVIIIIYFDQKKSEFLNEITYSFLGVSLDVPEKDKLKTKMEELIKKQINDIKDFTTIKKRIGKEISKELKVSIKRAVMNLFTKMTSKAPLILSTIISI", "aa_hexdigest": "c10c51f8547b12bdf68ad890009385fe", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557C32", "ncbi_nrp_id": "WP_234943733.1", "uniref100_id": "UniRef100_UPI0000557C32", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557C32", "RefSeq:WP_234943733.1", "UniRef:UniRef100_UPI0000557C32" ] }, "ips": { "uniref100_id": "UniRef100_UPI0000557C32", "uniref90_id": "UniRef90_P47662" }, "psc": { "uniref90_id": "UniRef90_P47662", "gene": "RnjA", "product": "Uncharacterized protein MG423", "uniref50_id": "UniRef50_P47662", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0046872", "GO:0003723" ] }, "pscc": { "uniref50_id": "UniRef50_P47662", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47662" ], "product": "Uncharacterized protein MG423" }, "genes": [ "rnjA" ], "id": "KKNAAMEMLJ_947", "locus": "GCA000027325_00947" }, { "type": "cds", "sequence": "contig_1", "start": 531056, "stop": 531217, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F5EBD4", "UniRef:UniRef90_UPI0002F5EBD4" ], "nt": "ATGGCTAAGATTAAATTCTTTGCGCTCGGCGGTCAAGATGAACGTGGTAAAAATTGCTATGTGCTTGAAATTGACAATGATGTGTTTATCTTTAATGTTGGTAGCTTAACACCAACAACAGCAGTTTTAGGTGTTAAAAAAATTATTCCTGATTTTAGTTGA", "aa": "MAKIKFFALGGQDERGKNCYVLEIDNDVFIFNVGSLTPTTAVLGVKKIIPDFS", "aa_hexdigest": "c9b5373594c7ede41c36b5c1622af55e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002F5EBD4", "query_cov": 1.0, "subject_cov": 0.803030303030303, "identity": 1.0, "score": 110.0, "evalue": 7.18e-31, "valid": true, "uniref50_id": "UniRef50_UPI0002F5EBD4" }, "pscc": { "uniref50_id": "UniRef50_UPI0002F5EBD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F5EBD4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5821.718500000001, "isoelectric_point": 6.024103355407715 }, "id": "KKNAAMEMLJ_948", "locus": "GCA000027325_00948" }, { "type": "cds", "sequence": "contig_1", "start": 531220, "stop": 531480, "strand": "-", "frame": 3, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_009885608.1", "SO:0001217", "UniParc:UPI0000134D0F", "UniRef:UniRef100_P47663", "UniRef:UniRef50_P47663", "UniRef:UniRef90_P47663" ], "nt": "ATGAAAATTGATAAAGAACAAATCATTAAGGCTCATCAACTTCACAAAAACGATGTTGGCAGTGTGCAAGTACAAATCTCTATATTAACAGATCAGATTAAAAAATTAACAGACCACCTGTTAGCAAACAAAAAGGATTTTATTTCTAAGCGTGGTTTATATACAAAGGTATCAAAAAGAAAACGGCTACTTAAATATCTAAAAGAACGTAATATTGAAACCTACCGTGATTTGATTAAAAATTTAAACCTCCGGGGTTAA", "aa": "MKIDKEQIIKAHQLHKNDVGSVQVQISILTDQIKKLTDHLLANKKDFISKRGLYTKVSKRKRLLKYLKERNIETYRDLIKNLNLRG", "aa_hexdigest": "6aeee8d790f9c955a6872e2df8a5c4fe", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134D0F", "ncbi_nrp_id": "WP_009885608.1", "uniref100_id": "UniRef100_P47663", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134D0F", "RefSeq:WP_009885608.1", "UniRef:UniRef100_P47663" ] }, "ips": { "uniref100_id": "UniRef100_P47663", "uniref90_id": "UniRef90_P47663" }, "psc": { "uniref90_id": "UniRef90_P47663", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P47663", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47663" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "KKNAAMEMLJ_949", "locus": "GCA000027325_00949" }, { "type": "cds", "sequence": "contig_1", "start": 531669, "stop": 532007, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "ATGGACTCAACCTTTCATGAGCTTGGGATCTCTCAAACTTTAATTGAAACGCTTAATGCGCTTCATATTAATAAGCCAACAAAAATTCAACAAATCTCTATCCCTCAGTTTTTATCAGAAAAAAACTTAATAGTTCACTCGCCAACAGGAACTGGTAAAACTGCTGCTTTTGCAATTCCCATAATTGAGAAGCTATTAAAAGAAGATCAAACAGCAAAACCAACTTTAGTAATTGCTCCAACAAGAGAATTAGTAGAACAGATTAAAACCACATTTTCAAATATTGCTAAAAATAAAAAACTAAGAATTATTAGTTTAATTGGTGGTGTACCTGCTTGA", "aa": "MDSTFHELGISQTLIETLNALHINKPTKIQQISIPQFLSEKNLIVHSPTGTGKTAAFAIPIIEKLLKEDQTAKPTLVIAPTRELVEQIKTTFSNIAKNKKLRIISLIGGVPA", "aa_hexdigest": "50c2997dd1d88c2d2a506f2a77ce5c13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 84, "aa_cov": 0.75, "hmm_cov": 0.9880952380952381, "evalue": 2.3682780086619133e-25, "score": 84.29902648925781, "start": 27, "stop": 110 } ], "seq_stats": { "molecular_weight": 12266.26289999998, "isoelectric_point": 9.573248863220215 }, "id": "KKNAAMEMLJ_950", "locus": "GCA000027325_00950" }, { "type": "cds", "sequence": "contig_1", "start": 532056, "stop": 533003, "strand": "+", "frame": 3, "gene": "srmB", "product": "putative ATP-dependent RNA helicase MG425", "db_xrefs": [ "COG:COG0513", "COG:L", "EC:3.6.4.13", "GO:0003689", "GO:0003724", "GO:0005524", "GO:0005829", "GO:0009409", "GO:0016887", "GO:0033592", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "SO:0001217", "UniRef:UniRef50_P47664", "UniRef:UniRef90_P47664" ], "nt": "ATGGGTAGAATTATGGATCTTTTAGAGCGTAAAGCAATTCATTTTAGCGATTTAGAACACCTAATTATTGATGAAGTTGATTTAATGTTAGACCGTGGTTTTAAAAAACAAATTTTTAATTTACTAGAACAAATCAATTCCTTTAAACAAATTGCTGTTTATTCAGCTAGTTACAACCAAGAAGCTATTAACATTGCCAAGCAAATTACTAATAATGGGATCTTTATTGGATCACCTGAATTTAATAAAGACGCAAATACCAATAATGATAAACTAATCAAACAATTTGTTTGTTATCTATTTTCAGATCAAAAAAAGCAAGCTTTATACAGCCTTATAAAAACAGCACAAGTTAAGTCAATCATTGTTTTTTGTGACACTAAAAAACTAGTTGATGATCTTCATGTATTTTTAAGAAAAAATGAATTAAGAACTTTTGCACTTCATGGTGATAAAAAACAATTTATTAGAGAGAGAAATCTTAAAATCTTTGCCAATACAAAACAACCCACGATTCTAGTAACTACTGATCTTATTGGTCGTGGTATCCATGTTGAAGCAATCGATATGGTTATCAATTATTCAGCTTGTTTAAATCTAGAAGCTTATATAAATAGAATGGGAAGGACTGGCAGAAACAATCATAAAGGGACATGTGTAACTTTCTGCACCTCACAAGAAAAGAAAGTCTTTCTGAAAATGGTTGAGAAAATCACTGATAATCGAATAGCTGAATGTAAACAAATGGAAATAAAGTTAATTCCTTTAAAAAATAAAGCTAAAACTAAAAAAGGTGGTATTTCACTTGATTGTGTTCAGAAAATATATGCCAATGCAAAACCATATGACCGTAATAAACGTGTCCCTTTAGCAAGTGATCTTTTCAAAAGTCGTATGCGCCAGCCTGAAAAAGCTATGCAAAAGCAAAAAATTCATGACAATGACTGA", "aa": "MGRIMDLLERKAIHFSDLEHLIIDEVDLMLDRGFKKQIFNLLEQINSFKQIAVYSASYNQEAINIAKQITNNGIFIGSPEFNKDANTNNDKLIKQFVCYLFSDQKKQALYSLIKTAQVKSIIVFCDTKKLVDDLHVFLRKNELRTFALHGDKKQFIRERNLKIFANTKQPTILVTTDLIGRGIHVEAIDMVINYSACLNLEAYINRMGRTGRNNHKGTCVTFCTSQEKKVFLKMVEKITDNRIAECKQMEIKLIPLKNKAKTKKGGISLDCVQKIYANAKPYDRNKRVPLASDLFKSRMRQPEKAMQKQKIHDND", "aa_hexdigest": "5fdbd963bca825f461d3965ffe050405", "start_type": "ATG", "rbs_motif": null, "genes": [ "srmB" ], "truncated": "both", "pseudogene": { "start": 531756, "stop": 533018, "observations": { "mutation": [ 532005, 533001 ], "stop": [ 532005, 533001 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47664", "query_cov": 1.0, "subject_cov": 0.7015590200445434, "identity": 1.0, "score": 618.0, "evalue": 2.55e-221, "gene_start": 1, "gene_end": 315, "reference_start": 130, "reference_end": 444, "reference_sequence": "MDSTFHELGISQTLIETLNALHINKPTKIQQISIPQFLSEKNLIVHSPTGTGKTAAFAIPIIEKLLKEDQTAKPTLVIAPTRELVEQIKTTFSNIAKNKKLRIISLIGGVPAWKQIKKIKTNPQIIVGTMGRIMDLLERKAIHFSDLEHLIIDEVDLMLDRGFKKQIFNLLEQINSFKQIAVYSASYNQEAINIAKQITNNGIFIGSPEFNKDANTNNDKLIKQFVCYLFSDQKKQALYSLIKTAQVKSIIVFCDTKKLVDDLHVFLRKNELRTFALHGDKKQFIRERNLKIFANTKQPTILVTTDLIGRGIHVEAIDMVINYSACLNLEAYINRMGRTGRNNHKGTCVTFCTSQEKKVFLKMVEKITDNRIAECKQMEIKLIPLKNKAKTKKGGISLDCVQKIYANAKPYDRNKRVPLASDLFKSRMRQPEKAMQKQKIHDNDWQSNM" }, "paralog": false, "identity": 0.9952380952380953, "score": 818.0, "evalue": 8.42e-303, "description": "Internal stop codon at 532005, 533001. Nonsense mutation around 532005, 533001.", "psc": { "uniref90_id": "UniRef90_P47664", "gene": "SrmB", "product": "Probable ATP-dependent RNA helicase MG425", "ec_ids": [ "3.6.4.13" ], "uniref50_id": "UniRef50_P47664", "cog_id": "COG0513", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003724", "GO:0033592", "GO:0009409" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47664", "EC:3.6.4.13", "UniRef:UniRef50_P47664", "COG:COG0513", "COG:L", "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003724", "GO:0033592", "GO:0009409" ] }, "pscc": { "uniref50_id": "UniRef50_P47664", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47664" ], "product": "Probable ATP-dependent RNA helicase MG425" } }, "id": "KKNAAMEMLJ_951", "locus": "GCA000027325_00951" }, { "type": "cds", "sequence": "contig_1", "start": 533041, "stop": 533235, "strand": "+", "frame": 1, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_009885606.1", "SO:0001217", "UniParc:UPI0000133E3F", "UniRef:UniRef100_P47665", "UniRef:UniRef50_Q6MTC5", "UniRef:UniRef90_P47665" ], "nt": "ATGGCAAAAAAAGACCAACTTACCTTAAGAGGGCCTTTGTATGGCAATAATCGTTCTCATTCCAAAACTATTACAAGAAGAAAATGGAATGTAAACCTTCAGTCATGCAAAATTAAAGATACTAATGGTAAAGTAACACGGATTCTAGTTTCAACTAAAACAATTCGTACCCTTAAAAAACAAAATCGTTTCTAA", "aa": "MAKKDQLTLRGPLYGNNRSHSKTITRRKWNVNLQSCKIKDTNGKVTRILVSTKTIRTLKKQNRF", "aa_hexdigest": "e60ec7143344c99d1a5fc9f7fa1f4c04", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133E3F", "ncbi_nrp_id": "WP_009885606.1", "uniref100_id": "UniRef100_P47665", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133E3F", "RefSeq:WP_009885606.1", "UniRef:UniRef100_P47665" ] }, "ips": { "uniref100_id": "UniRef100_P47665", "uniref90_id": "UniRef90_P47665" }, "psc": { "uniref90_id": "UniRef90_P47665", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q6MTC5", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MTC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MTC5" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "KKNAAMEMLJ_952", "locus": "GCA000027325_00952" }, { "type": "cds", "sequence": "contig_1", "start": 533271, "stop": 533561, "strand": "+", "frame": 3, "gene": "yhfA", "product": "Hydroperoxide reductase", "db_xrefs": [ "COG:COG1765", "COG:R", "EC:1.11.1.-", "GO:0004601", "GO:0005737", "SO:0001217", "UniRef:UniRef50_P47666", "UniRef:UniRef90_P47666" ], "nt": "ATGGATAAAAAATACGATATCACAGCTGTTTTGAACGATGATAGTTCTATTAATGCAGTAAGCGATAACTTCCAAATAACACTGGATGCACGACCAAAAGAAAAATCTAAAGGCATTAATCCTCTGTCAGCTTTTTTGGCTGGTTTAGCTGCATGTGAACTTGCAACTGCTAATGCGATGGCAGCTGCTAAGATGATAACTTTGAACAAGGCACTGATTAACATCAAAGGTTATCGTTTAACAAATCCTAGTGATGGTTATTTTGGCCTACGTGAACTTAACATTCACTGA", "aa": "MDKKYDITAVLNDDSSINAVSDNFQITLDARPKEKSKGINPLSAFLAGLAACELATANAMAAAKMITLNKALINIKGYRLTNPSDGYFGLRELNIH", "aa_hexdigest": "e50dd3359b8c28aa987740bc630a4faf", "start_type": "ATG", "rbs_motif": null, "genes": [ "yhfA" ], "truncated": "3-prime", "pseudogene": { "start": 533271, "stop": 533696, "observations": { "mutation": [ 533559 ], "stop": [ 533559 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47666", "query_cov": 1.0, "subject_cov": 0.6808510638297872, "identity": 1.0, "score": 185.0, "evalue": 1.09e-58, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "MDKKYDITAVLNDDSSINAVSDNFQITLDARPKEKSKGINPLSAFLAGLAACELATANAMAAAKMITLNKALINIKGYRLTNPSDGYFGLRELNIHWEIHSPNEEEEIKEFIDFVSKRCPAHNTLHGTSNFKINISVTLVH" }, "paralog": false, "identity": 0.9929078014184397, "score": 278.0, "evalue": 4.28e-98, "description": "Internal stop codon at 533559. Nonsense mutation around 533559.", "psc": { "uniref90_id": "UniRef90_P47666", "gene": "YhfA", "product": "Hydroperoxide reductase", "ec_ids": [ "1.11.1.-" ], "uniref50_id": "UniRef50_P47666", "cog_id": "COG1765", "cog_category": "R", "go_ids": [ "GO:0005737", "GO:0004601" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47666", "EC:1.11.1.-", "UniRef:UniRef50_P47666", "COG:COG1765", "COG:R", "GO:0005737", "GO:0004601" ] }, "pscc": { "uniref50_id": "UniRef50_P47666", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47666" ], "product": "Hydroperoxide reductase" } }, "id": "KKNAAMEMLJ_953", "locus": "GCA000027325_00953" }, { "type": "cds", "sequence": "contig_1", "start": 534026, "stop": 534322, "strand": "+", "frame": 2, "gene": "gerE", "product": "putative protein MG428", "db_xrefs": [ "COG:COG5905", "COG:DK", "GO:0003700", "GO:0006352", "SO:0001217", "UniRef:UniRef50_P47667", "UniRef:UniRef90_P47667" ], "nt": "ATGGTGAATAAAGTTGAATTGCTTGATTCTAAAAGTAAATACTCATTAGAAAAGCAAAACACAGTTCTAAATACACCTGAAAACTATTTACGGAGTTTAGAATTTAAAGAAATTATTACTAAAGCTTTTAATAAAGCTAAAAACGATCAAGAGAGAAAAGTTTTTTCTTTATATGTAAAGGGCTATAAAAACTTTGAGATAGCAAAAAAGCTCAATATTAGTCCTAGAAGAGTGAGATATTTATTAGATCTTTTTAAAAGCTACATCAAATTGCTAACAGAAAGATATGGATATTAA", "aa": "MVNKVELLDSKSKYSLEKQNTVLNTPENYLRSLEFKEIITKAFNKAKNDQERKVFSLYVKGYKNFEIAKKLNISPRRVRYLLDLFKSYIKLLTERYGY", "aa_hexdigest": "a60561533f3a89e19fe4e179790c3501", "start_type": "ATG", "rbs_motif": null, "genes": [ "gerE" ], "truncated": "5-prime", "pseudogene": { "start": 533807, "stop": 534322, "observations": { "mutation": [ 533861, 533870 ], "stop": [ 533861, 533870 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47667", "query_cov": 1.0, "subject_cov": 0.5730994152046783, "identity": 1.0, "score": 188.0, "evalue": 1.88e-59, "gene_start": 1, "gene_end": 98, "reference_start": 74, "reference_end": 171, "reference_sequence": "MKNNISDVKLGLLAAKIYWKSWRFLELTEDDIISIALHAEQDSKKRFNPEFGLSFDNYLKLNGANFIRSSFRSMVNKVELLDSKSKYSLEKQNTVLNTPENYLRSLEFKEIITKAFNKAKNDQERKVFSLYVKGYKNFEIAKKLNISPRRVRYLLDLFKSYIKLLTERYGY" }, "paralog": false, "identity": 0.9883040935672515, "score": 328.0, "evalue": 3.16e-117, "description": "Internal stop codon at 533861, 533870. Nonsense mutation around 533861, 533870.", "psc": { "uniref90_id": "UniRef90_P47667", "gene": "GerE", "product": "Uncharacterized protein MG428", "uniref50_id": "UniRef50_P47667", "cog_id": "COG5905", "cog_category": "DK", "go_ids": [ "GO:0003700", "GO:0006352" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47667", "UniRef:UniRef50_P47667", "COG:COG5905", "COG:DK", "GO:0003700", "GO:0006352" ] }, "pscc": { "uniref50_id": "UniRef50_P47667", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47667" ], "product": "Uncharacterized protein MG428" } }, "id": "KKNAAMEMLJ_954", "locus": "GCA000027325_00954" }, { "type": "cds", "sequence": "contig_1", "start": 534319, "stop": 534549, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "ATGTGTGGTGAAATGGCCAGTGATCAATATGCAATACCGTTGCTTTTAGGTTTAGGGTTAACAGAGTTATCAATGAGTGCAAGTTCAATGTTTAAAGCAAGAATGGTTATTGCTAAAATAACAATTAATGAATGTAAATCATTAGTTGAAAAAGCATTGAAATTAACTAGTGATAGTGCTGTTAGAAAACTAGTAGAAAATTTCTTTAAAAAGAAAAATATCTTTATTTAA", "aa": "MCGEMASDQYAIPLLLGLGLTELSMSASSMFKARMVIAKITINECKSLVEKALKLTSDSAVRKLVENFFKKKNIFI", "aa_hexdigest": "7507b1111836ac45260a9c1b52b55ee4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 40, "aa_cov": 0.5263157894736842, "hmm_cov": 1.0, "evalue": 3.708362957959849e-11, "score": 37.77103805541992, "start": 1, "stop": 40 } ], "seq_stats": { "molecular_weight": 8417.043499999992, "isoelectric_point": 9.301256752014158 }, "id": "KKNAAMEMLJ_955", "locus": "GCA000027325_00955" }, { "type": "cds", "sequence": "contig_1", "start": 534556, "stop": 535053, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "TTGGTAATAATTAGAACATTAGACATTGGTGGAGATAAAAAACTAAATTATTTTCAATTTCCTCATGAAGATAACCCTTTCTTAGGTTATCGTGCTATCCGTTTAACATTAGATAAACAAGCTGTTTTTAAGACCCAACTTCGTGCTTTATTAAGAGCTTCTGATTATGGAAATTTAGGAATTATGTTTCCAATGGTTGCAACTCTTGATGAATTAGTTCAAGTTAAGCAACTTTTAACAAAAGTTCAACAAGAATTTAATGAAACTAAGAAATTTAAATTAGGAATAATGATTGAAATTCCATCAGCAGCATTAGCAGCTGATTGCTTAGGAAAACATGTTGATTTTTTCTCAATAGGTAGCAATGATTTAATCCAATATAGTTTTGCTGCTGATAGGATGAATAAAAACGTTAGTTATCTATACCAACCATTAAATCCAGCATTACTACGCTTAATTAAACTAGTTGTAGAAGGTGGTAAATTAAATAATGTTTGA", "aa": "MVIIRTLDIGGDKKLNYFQFPHEDNPFLGYRAIRLTLDKQAVFKTQLRALLRASDYGNLGIMFPMVATLDELVQVKQLLTKVQQEFNETKKFKLGIMIEIPSAALAADCLGKHVDFFSIGSNDLIQYSFAADRMNKNVSYLYQPLNPALLRLIKLVVEGGKLNNV", "aa_hexdigest": "5720b2644b7a7a4a680dc830d9719160", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 164, "aa_cov": 0.9636363636363636, "hmm_cov": 1.0, "evalue": 4.8522150355026945e-70, "score": 231.05059814453125, "start": 2, "stop": 160 } ], "seq_stats": { "molecular_weight": 18667.6638, "isoelectric_point": 9.161424827575683 }, "id": "KKNAAMEMLJ_956", "locus": "GCA000027325_00956" }, { "type": "cds", "sequence": "contig_1", "start": 535114, "stop": 535302, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "TTGGAATCCAACTTTCAAAATGAATTAAAACAATATACCAATAAATTAGTAAAAACCTTAGATGGATATGAAGTTATTGTCGCTTCTAATATTGGTAATGTAAAAGATATGGATTTAGCAGTTGAGTATAACACGAATGGAATTGGTCTTTTTAGAACTGAATTTCTCTATATGAGTAGTCAAGATTGA", "aa": "MESNFQNELKQYTNKLVKTLDGYEVIVASNIGNVKDMDLAVEYNTNGIGLFRTEFLYMSSQD", "aa_hexdigest": "d56c37f0a4f39f9dad85ac763f34f5bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 54, "aa_cov": 0.8709677419354839, "hmm_cov": 1.0, "evalue": 1.0912058194562304e-11, "score": 39.51490783691406, "start": 8, "stop": 61 } ], "seq_stats": { "molecular_weight": 7100.877000000001, "isoelectric_point": 4.4865518569946286 }, "id": "KKNAAMEMLJ_957", "locus": "GCA000027325_00957" }, { "type": "cds", "sequence": "contig_1", "start": 535318, "stop": 536037, "strand": "-", "frame": 3, "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "db_xrefs": [ "COG:COG1080", "COG:G", "EC:2.7.3.9", "GO:0005737", "GO:0008965", "GO:0009401", "GO:0016301", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47668", "UniRef:UniRef90_P47668" ], "nt": "ATGAAAAAAATAATTGGGATTGGTGTTTCAGATGGCATTGCTGTTGCAAAAGCTTTCATTATTCAAACACCTCAATTTGACGTTAAAAAATACACAAATGTCAAAATGACACCCACCCAAGCAAAAAAGCTTTTAAGCAGTGCTTTTCAAAAAGCAAAAAAAGACCTTGAAGAGATTAAGACAATTACTGTTAAGAATATTAATCAAGAAGCAGGAATGATTTTTGATGCACACATCCAAATTCTTAATGATCCTACTATCACTGAGCAATTAGAACAACAACTAAATAAAAACATCCATCCTGTTATTGCTGTTGATAATGTTTTTCAACAAACAGCTTTAATGTTTAGTGAAATGGATGATAAGTATTTTAAAGAACGTGCCAGTGATATATTAGATTTACACCAAAGGCTCTTAAGTTATTTAACTGGAGTAAAACTTAATGATTTAATCAGGATTAAAAGTGATGTCATTATAGTAGCTAATGATTTAACACCAAGTCAAACAGCAACTCTTAATAAAAAGTATGTCAAGGGTTTTTTAACTGAGAGTGGTGGTAAAACAAGTCATGCTGCCATTATGGCTCGTTCTATGGAAATTCCTGCCATAGTTGGTTTAAAAAATATTACCAGTAAAGTTGAAGATGGCAAAACAGTTGGTATCAATGGTAGAAAAGGTATTGTGGGTTTTGACTTTTCAAGTAAAGACATAACTCAATGA", "aa": "MKKIIGIGVSDGIAVAKAFIIQTPQFDVKKYTNVKMTPTQAKKLLSSAFQKAKKDLEEIKTITVKNINQEAGMIFDAHIQILNDPTITEQLEQQLNKNIHPVIAVDNVFQQTALMFSEMDDKYFKERASDILDLHQRLLSYLTGVKLNDLIRIKSDVIIVANDLTPSQTATLNKKYVKGFLTESGGKTSHAAIMARSMEIPAIVGLKNITSKVEDGKTVGINGRKGIVGFDFSSKDITQ", "aa_hexdigest": "76bf1acaedda6abaf39537fab7eb4675", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsI" ], "truncated": "5-prime", "pseudogene": { "start": 535015, "stop": 536037, "observations": { "mutation": [ 535116, 535320 ], "stop": [ 535116, 535320 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47668", "query_cov": 1.0, "subject_cov": 0.4178321678321678, "identity": 1.0, "score": 457.0, "evalue": 2.69e-157, "gene_start": 1, "gene_end": 239, "reference_start": 1, "reference_end": 239, "reference_sequence": "MKKIIGIGVSDGIAVAKAFIIQTPQFDVKKYTNVKMTPTQAKKLLSSAFQKAKKDLEEIKTITVKNINQEAGMIFDAHIQILNDPTITEQLEQQLNKNIHPVIAVDNVFQQTALMFSEMDDKYFKERASDILDLHQRLLSYLTGVKLNDLIRIKSDVIIVANDLTPSQTATLNKKYVKGFLTESGGKTSHAAIMARSMEIPAIVGLKNITSKVEDGKTVGINGRKGIVGFDFSSKDITQWKQEKELESNFQNELKQYTNKLVKTLDGYEVIVASNIGNVKDMDLAVEYNTNGIGLFRTEFLYMSSQDWPDESVQFEAYKTVLQKAKNDLVIIRTLDIGGDKKLNYFQFPHEDNPFLGYRAIRLTLDKQAVFKTQLRALLRASDYGNLGIMFPMVATLDELVQVKQLLTKVQQEFNETKKFKLGIMIEIPSAALAADCLGKHVDFFSIGSNDLIQYSFAADRMNKNVSYLYQPLNPALLRLIKLVVEGGKLNNVWTGMCGEMASDQYAIPLLLGLGLTELSMSASSMFKARMVIAKITINECKSLVEKALKLTSDSAVRKLVENFFKKKNIFI" }, "paralog": false, "identity": 0.9941176470588236, "score": 651.0, "evalue": 4.21e-236, "description": "Internal stop codon at 535116, 535320. Nonsense mutation around 535116, 535320.", "psc": { "uniref90_id": "UniRef90_P47668", "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "ec_ids": [ "2.7.3.9" ], "uniref50_id": "UniRef50_P47668", "cog_id": "COG1080", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47668", "EC:2.7.3.9", "UniRef:UniRef50_P47668", "COG:COG1080", "COG:G", "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47668", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47668" ], "product": "Phosphoenolpyruvate-protein phosphotransferase" } }, "id": "KKNAAMEMLJ_958", "locus": "GCA000027325_00958" }, { "type": "cds", "sequence": "contig_1", "start": 536041, "stop": 537564, "strand": "-", "frame": 3, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0005975", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537", "KEGG:K15633", "RefSeq:WP_009885603.1", "SO:0001217", "UniParc:UPI0000557C28", "UniRef:UniRef100_P47669", "UniRef:UniRef50_P75167", "UniRef:UniRef90_P47669" ], "nt": "ATGCATAAAAAAGTTTTATTAGCAATCCTTGATGGTTATGGGATCTCAAATGCTATTTATGGTAATGCAGTACAAAATGCAAATACCCCAATGCTAGATGAATTAATCAATTCATATCCTTGTGTACTTTTAGATGCATCTGGGGAAGCAGTTGGATTGCCTATGGGTCAAATAGGTAACTCTGAGGTAGGTCATCTAAATATTGGGGCAGGTCGAGTTGTTTATACTGGACTTTCTTTGATTAATCAACATATTAAGGATCGTAGTTTTTTTGCAAATAAAGCTTTTTTAAAAACCATAGAACATGTAGAAAAAAACCATTCAAAAATCCATTTAATTGGGTTATTTTCCAATGGAGGAGTGCATAGTCATAATGAACATCTATTAGCACTCATTGAATTGTTTTCAAAACATGCAAAGGTAGTATTACATTTATTTGGTGATGGTAGAGATGTAGCACCTTGTAGCTTAAAACAAGATCTTGAGAAATTAATGATATTTCTAAAAAACTATCCTAATGTTGTTATTGGAACTATTGGGGGAAGATACTATGGAATGGATCGTGATCAACGCTGGGATCGTGAAATGATTGCTTATAAAGCTTTATTAGGAGTTTCAAAAAATAAATTCAATGACCCAATTGGTTATATTGAAACGCAATATCAGAACCAAATTACTGATGAATTTATTTATCCTGCAATTAATGCCAATTTAAATTCTGATCAGTTTGCATTAAACAATAATGATGGAGTTATTTTCTTTAATTTTAGACCTGATAGAGCAAGACAAATGTCCCATTTGATCTTTAACAGCAATTATTACAACTATCAACCTGAATTGAAACGAAAAGAAAATTTATTTTTTGTAACAATGATGAATTATGAGGGAATTGTACCTAGCGAATTTGCTTTTCCACCTCAAACCATTAAAAATAGTCTTGGTGAAGTAATTGCTAATAATAATTTGAAGCAATTGAGGATTGCAGAAACTGAAAAGTATGCTCACGTTACTTTCTTTTTTGATGGTGGTTTTGAAGTTAATCTCAGCAATGAAACAAAGACATTAATTCCTTCTTTAAAAGTTGCTACATATGATTTAGCTCCCGAAATGTCATGTAAAGCTATTACTGATGCACTACTAGAAAAGCTTAATAACTTTGATTTTACTGTTTTAAATTTTGCTAATCCTGATATGGTAGGTCATACTGGTAACTATCAAGCTTGCATTAAAGCTCTTGAAGCACTCGATGTTCAAATTAAACGAATAGTTGATTTTTGTAAAGCTAATCAAATAACTATGTTTTTAACTGCAGATCATGGGAATGCAGAAGTGATGATTGATAATAATAACAATCCAGTTACTAAACACACTATTAATCCTGTACCATTTGTATGTACTGACAAAAATGTTAACTTTAATCAAACTGGAATTTTAGCTAATATTGCTCCTACTATCTTGGAATATCTTAACCTTAGCAAACCAAAAGAGATGACTGCAAAATCCTTATTAAAAAATAACAATTAA", "aa": "MHKKVLLAILDGYGISNAIYGNAVQNANTPMLDELINSYPCVLLDASGEAVGLPMGQIGNSEVGHLNIGAGRVVYTGLSLINQHIKDRSFFANKAFLKTIEHVEKNHSKIHLIGLFSNGGVHSHNEHLLALIELFSKHAKVVLHLFGDGRDVAPCSLKQDLEKLMIFLKNYPNVVIGTIGGRYYGMDRDQRWDREMIAYKALLGVSKNKFNDPIGYIETQYQNQITDEFIYPAINANLNSDQFALNNNDGVIFFNFRPDRARQMSHLIFNSNYYNYQPELKRKENLFFVTMMNYEGIVPSEFAFPPQTIKNSLGEVIANNNLKQLRIAETEKYAHVTFFFDGGFEVNLSNETKTLIPSLKVATYDLAPEMSCKAITDALLEKLNNFDFTVLNFANPDMVGHTGNYQACIKALEALDVQIKRIVDFCKANQITMFLTADHGNAEVMIDNNNNPVTKHTINPVPFVCTDKNVNFNQTGILANIAPTILEYLNLSKPKEMTAKSLLKNNN", "aa_hexdigest": "097c042439f6da10ae27224b1b6e3ca6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557C28", "ncbi_nrp_id": "WP_009885603.1", "uniref100_id": "UniRef100_P47669", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557C28", "RefSeq:WP_009885603.1", "UniRef:UniRef100_P47669" ] }, "ips": { "uniref100_id": "UniRef100_P47669", "uniref90_id": "UniRef90_P47669" }, "psc": { "uniref90_id": "UniRef90_P47669", "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_P75167", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0005975", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_P75167", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75167" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "KKNAAMEMLJ_959", "locus": "GCA000027325_00959" }, { "type": "cds", "sequence": "contig_1", "start": 537803, "stop": 538291, "strand": "-", "frame": 2, "gene": "tpiA", "product": "triose-phosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_P78010", "UniRef:UniRef90_P47670" ], "nt": "ATGCGCACAAGGTATTTAATTGGCAATTGGAAAACAAATAAAAATTTAAAAGACGCAGTTAGTTTTGTTGAACAATTTCAACAAAATAAACTTAATTACAATGCCAAAATTGGGATAGCACCTGTTTATGTTCATCTCACTGAAATAAAAAAAATAATTAGTGATAGTCTCCTTTTATTTGCCCAAGACGCTAACTTTATTGAAAGTGGTTCATATACTGGAACTGTAAGCTTTACTCAACTTCAAGACATTGGTGTTAACAACAGTATTATTGGTCATTCTGAAAGAAGAAAATACTATAACGAAACCAGTGCAGTTATTAATCAAAAGCTCTTTGCTTGTCTAAAAGCATCCATGCAAGTAGTTTTATGTATTGGTGAGGCTTTAGGACAAGAGATTAGCTTTCTTAAAACTGATCTTACTAATTGCTTAGATACGATTGACAAAAGCTTAATTAAAAATTTAGTTATTGCTTATGAACCTTTGTGA", "aa": "MRTRYLIGNWKTNKNLKDAVSFVEQFQQNKLNYNAKIGIAPVYVHLTEIKKIISDSLLLFAQDANFIESGSYTGTVSFTQLQDIGVNNSIIGHSERRKYYNETSAVINQKLFACLKASMQVVLCIGEALGQEISFLKTDLTNCLDTIDKSLIKNLVIAYEPL", "aa_hexdigest": "5cf18151a5dbc8ac21e7a780e95117e6", "start_type": "ATG", "rbs_motif": null, "genes": [ "tpiA" ], "truncated": "5-prime", "pseudogene": { "start": 537557, "stop": 538291, "observations": { "mutation": [ 537805 ], "stop": [ 537805 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47670", "query_cov": 1.0, "subject_cov": 0.6639344262295082, "identity": 1.0, "score": 320.0, "evalue": 1.77e-109, "gene_start": 1, "gene_end": 162, "reference_start": 1, "reference_end": 162, "reference_sequence": "MRTRYLIGNWKTNKNLKDAVSFVEQFQQNKLNYNAKIGIAPVYVHLTEIKKIISDSLLLFAQDANFIESGSYTGTVSFTQLQDIGVNNSIIGHSERRKYYNETSAVINQKLFACLKASMQVVLCIGEALGQEISFLKTDLTNCLDTIDKSLIKNLVIAYEPLWAIGTGKTATPEVANQTIKTIREYINDLYDENVANNISILYGGSVDHNNIQKLAIMEQIDGFLVGKASLEIKNFLEMARVYA" }, "paralog": false, "identity": 0.9959016393442623, "score": 475.0, "evalue": 3.56e-173, "description": "Internal stop codon at 537805. Nonsense mutation around 537805.", "psc": { "uniref90_id": "UniRef90_P47670", "gene": "tpiA", "product": "triose-phosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_P78010", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47670", "EC:5.3.1.1", "UniRef:UniRef50_P78010", "COG:COG0149", "COG:G", "KEGG:K01803", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ] }, "pscc": { "uniref50_id": "UniRef50_P78010", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78010" ], "product": "Triosephosphate isomerase" } }, "id": "KKNAAMEMLJ_960", "locus": "GCA000027325_00960" }, { "type": "cds", "sequence": "contig_1", "start": 538351, "stop": 538563, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "ATGTTTGAAGCTGAAGGAGGATATTCACGCCAAAAAACCCAAGTGCTTGTAACTAATTGTTTGCTTATTAAAGCTGCTAAGCTTTTAGAAGATGTGAGAAAGTTTGATCGTGATGCTTTGTTTTCCATTACTTTTATTAAAAAGCTTGATGGCTATATCTATGATAGACGTACCAATAAGCAAACAAAACATGGAACTGAAAACAAGTCATAA", "aa": "MFEAEGGYSRQKTQVLVTNCLLIKAAKLLEDVRKFDRDALFSITFIKKLDGYIYDRRTNKQTKHGTENKS", "aa_hexdigest": "ceb0fef955ca27160845bc926d8f4be4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 49, "aa_cov": 0.7, "hmm_cov": 1.0, "evalue": 2.6818345780299517e-09, "score": 31.88802719116211, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 8143.274599999996, "isoelectric_point": 9.560741996765138 }, "id": "KKNAAMEMLJ_961", "locus": "GCA000027325_00961" }, { "type": "cds", "sequence": "contig_1", "start": 538576, "stop": 538851, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTAATACAGTTAGCTTTATTATTGGCTATGTAATTGGTACTTATTTAACTGGTAGTTTATCAGTGCAATCTTATGTTGGTGATGATAAACACCAGCCATTTGGAGTTGCTTTCTTTTTATCTCCAAACCTCGTTTTTACTCTATTGATGAACATTGTGTTGGGATTATTTACTTCTTTTTATTTCCCTAAGTATCAATTTGTAAAAGTAGAAGTTTATGGTAAGCACATAGAAAAAATTCGTAACTATCTACTTGATAATCAACAGTGA", "aa": "MLINTVSFIIGYVIGTYLTGSLSVQSYVGDDKHQPFGVAFFLSPNLVFTLLMNIVLGLFTSFYFPKYQFVKVEVYGKHIEKIRNYLLDNQQ", "aa_hexdigest": "a3d3a56c52259c9c7a9bcb3893067ea4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10451.071599999992, "isoelectric_point": 7.983909797668457 }, "id": "KKNAAMEMLJ_962", "locus": "GCA000027325_00962" }, { "type": "cds", "sequence": "contig_1", "start": 539023, "stop": 539355, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTCCATTAGCCAAGGATTAGCTCGTCTTGTTAATTTTTTTATTACTTCACAAAATATTAATGTTGATTCAGCAACTATATTTAATGCTATTTTTTGGTTAACACAAATTCTTTTTAATGTTCCTTTCTTTATTTTTGGATGGTTTAAAATCTCCAAAAAATTTACCCTATTAACCCTTTATTTTGTAGCTGTTTCAAATCTTTTTGGTTTTTTCTTTTCATACATCCCTGGAATAGATAACTTCTTTTTGTTTGCAAATTTAACTACAGCAAAAGATGGTGGATTTGAAAATTTAATTAATGAAAAGGGAGTACAACTTATATTTTGA", "aa": "MASISQGLARLVNFFITSQNINVDSATIFNAIFWLTQILFNVPFFIFGWFKISKKFTLLTLYFVAVSNLFGFFFSYIPGIDNFFLFANLTTAKDGGFENLINEKGVQLIF", "aa_hexdigest": "d07475790e5d554120dc696c3924d2e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12564.50529999998, "isoelectric_point": 8.063850593566894 }, "id": "KKNAAMEMLJ_963", "locus": "GCA000027325_00963" }, { "type": "cds", "sequence": "contig_1", "start": 539633, "stop": 539749, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACGAAAATAGAGCTAATAAAAGAATTGCGTAAATCAACACAAGCAAGTGTTATGGATTGTAAACAAGCTTTGGAAAAAAATAATGATGATTTTGAGAAAGCTGTTAAGTGA", "aa": "MATKIELIKELRKSTQASVMDCKQALEKNNDDFEKAVK", "aa_hexdigest": "a22b1c8f6cd0bba9263dc05eb1fb1408", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4355.0216, "isoelectric_point": 7.870574378967285 }, "id": "KKNAAMEMLJ_964", "locus": "GCA000027325_00964" }, { "type": "cds", "sequence": "contig_1", "start": 539846, "stop": 540238, "strand": "+", "frame": 2, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "SO:0001217", "UniRef:UniRef50_P47246", "UniRef:UniRef90_P47246" ], "nt": "ATGGTTGAGATAAACTCACAAACTGATTTTGTAGCCAAAAATCAAGAGTTAAAAGAATTTTCAGATTTAATGCTTGAAAAAATATTTGAAAAAGTAAATCCAAAAACAGAATTAGTTGAAATTGAAAAAATTCAAATTAATAATGATGAAAAAGTTAGTGAAAAACTAGCATTAATTGCTTCTAAAACTGATGAGAAAATAGTACTTAGAAGAGTAGTTGTATTTGAAACTAAAACTAATCAAATTTTCACCTATTTACATGCCAATAAAAGAATTGGGGTAATTATTGAGATTCAAGGAAAACTCAACGAAGATGATGGTAAGCATTTAGCAATGCATATTGCTGCTAATTCACCACAATTTATTGATCAAAGTGATGTTAATCAAACATGA", "aa": "MVEINSQTDFVAKNQELKEFSDLMLEKIFEKVNPKTELVEIEKIQINNDEKVSEKLALIASKTDEKIVLRRVVVFETKTNQIFTYLHANKRIGVIIEIQGKLNEDDGKHLAMHIAANSPQFIDQSDVNQT", "aa_hexdigest": "c5609d87f049217d1d811b231ae7842f", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsf" ], "truncated": "both", "pseudogene": { "start": 539633, "stop": 540529, "observations": { "mutation": [ 539747, 540236 ], "stop": [ 539747, 540236 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47246", "query_cov": 1.0, "subject_cov": 0.436241610738255, "identity": 1.0, "score": 244.0, "evalue": 1.81e-79, "gene_start": 1, "gene_end": 130, "reference_start": 72, "reference_end": 201, "reference_sequence": "MATKIELIKELRKSTQASVMDCKQALEKNNDDFEKAVKWLRENGIVKSTKKLNKVASEGIIVLKSNLHKAIMVEINSQTDFVAKNQELKEFSDLMLEKIFEKVNPKTELVEIEKIQINNDEKVSEKLALIASKTDEKIVLRRVVVFETKTNQIFTYLHANKRIGVIIEIQGKLNEDDGKHLAMHIAANSPQFIDQSDVNQTWLQNERNIIRSQAELEVKENPKKAIFLEKTIEGRVNKLLIDTCLINQKYLIDETKTIGQFLKEKQAKVLKFIRYEVGEGIIKETVDFVSEVNAQIKQ" }, "paralog": false, "identity": 0.9932885906040269, "score": 562.0, "evalue": 3.97e-207, "description": "Internal stop codon at 539747, 540236. Nonsense mutation around 539747, 540236.", "psc": { "uniref90_id": "UniRef90_P47246", "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_P47246", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47246", "UniRef:UniRef50_P47246", "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_P47246", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47246" ], "product": "Elongation factor Ts" } }, "id": "KKNAAMEMLJ_965", "locus": "GCA000027325_00965" }, { "type": "cds", "sequence": "contig_1", "start": 540510, "stop": 541241, "strand": "+", "frame": 3, "gene": "pyrH", "product": "UMP kinase", "db_xrefs": [ "COG:COG0528", "COG:F", "EC:2.7.4.22", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210", "KEGG:K09903", "RefSeq:WP_009885599.1", "SO:0001217", "UniParc:UPI0000132DAF", "UniRef:UniRef100_P47672", "UniRef:UniRef50_P47672", "UniRef:UniRef90_P47672" ], "nt": "ATGCACAAATCAAACAATAAAATCCGCCAAAGAATAATCATTAAACTTAGTGGTGCTGGGCTAACCAAAGAAAATTCTCAACCCTTTTCTAATGATTTTTTTGAAACTATTATTAATCAATTAAAAGTTTTAAAAGAAAGCTATCAAGTAGGAATTGTTATTGGTGGGGGTAACATTATCAGAGGTAATAATTGCCAAGAATTTAACATTGCTGAATACCATGGTCATCAACTTGGTATTATAGCAACAGTAGTTAATGGCTATTTTTTAAAAGCAAAGTTAGATGCACATAATTTGAAAAGTGCTTTACTAAGTGCAATTAGTTGTCCTAGTTTAGCAGTGCAAATTCTTTCACAGCAAACTATTGATAAAGCTTTTGAAGAGAATGACTTTGTCATTTTTTCAGGTGGCACTGGTAATCCTTATTTTTCCACTGACACTGCATTAGCTTTAAGAGCAGTGCAAACAAAAGCAGTTGCTATTCTGATTGGAAAAAATGGTGTTGATGGTGTTTATACAGCTGATCCTAAAAAAGATAAAAATGCAACCTTTTTACCAACACTCAACTATGACCATGCCATTAAAAATGATTTGAAAATTATGGATATTACTGCTTTTACTATGTGTAAGGAAAATAATCTGAAAATAATTATTTTTAACATTAATGCTGAGAATGCATTATTAGATGCATTAAACAAAAAAGGTCGCTTTACTATAATTGAAAATAACTAA", "aa": "MHKSNNKIRQRIIIKLSGAGLTKENSQPFSNDFFETIINQLKVLKESYQVGIVIGGGNIIRGNNCQEFNIAEYHGHQLGIIATVVNGYFLKAKLDAHNLKSALLSAISCPSLAVQILSQQTIDKAFEENDFVIFSGGTGNPYFSTDTALALRAVQTKAVAILIGKNGVDGVYTADPKKDKNATFLPTLNYDHAIKNDLKIMDITAFTMCKENNLKIIIFNINAENALLDALNKKGRFTIIENN", "aa_hexdigest": "b21388d51fa95f76184a6f024415a5ea", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000132DAF", "ncbi_nrp_id": "WP_009885599.1", "uniref100_id": "UniRef100_P47672", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000132DAF", "RefSeq:WP_009885599.1", "UniRef:UniRef100_P47672" ] }, "ips": { "uniref100_id": "UniRef100_P47672", "uniref90_id": "UniRef90_P47672" }, "psc": { "uniref90_id": "UniRef90_P47672", "gene": "pyrH", "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_P47672", "cog_id": "COG0528", "cog_category": "F", "kegg_orthology_id": "K09903", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ] }, "pscc": { "uniref50_id": "UniRef50_P47672", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47672" ], "product": "Uridylate kinase" }, "genes": [ "pyrH" ], "id": "KKNAAMEMLJ_966", "locus": "GCA000027325_00966" }, { "type": "cds", "sequence": "contig_1", "start": 541364, "stop": 541792, "strand": "+", "frame": 2, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023", "SO:0001217", "UniRef:UniRef50_P47673", "UniRef:UniRef90_P47673" ], "nt": "TTGATTGAAAGTTATGGACAAAAAATGCCTTTAATATCTTTAGCTCAAGTGACTATTAATCCGCCAAGAGAAATAATCATAAAACCATTTGATCCTAAGAGTAATACTAATGCTATTTACAGTGAAATTCAGCGGGCAAACATTGGTGTTCAACCAGTTATTGATGGTGAAAAAATTCGTGTTAATTTTCCCCAAATTACTCAAGAAACTCGCTTAGAAAATATTAAGCACGTTAAAAAAATAATAGAGCAAATTTATCAAGAACTGAGGGTTGTAAGAAGAGATGCATTACAAATGATTAAAAAAGATAATCACAATGAGGATTTAGAAAACTCTTTAAAAGCTGAAATAGAAAAAATTAACAAAAATTATTCTAATCAATTAGAAGAGATTCAAAAAGACAAAGAAAAAGAATTGCTAACAATTTAA", "aa": "MIESYGQKMPLISLAQVTINPPREIIIKPFDPKSNTNAIYSEIQRANIGVQPVIDGEKIRVNFPQITQETRLENIKHVKKIIEQIYQELRVVRRDALQMIKKDNHNEDLENSLKAEIEKINKNYSNQLEEIQKDKEKELLTI", "aa_hexdigest": "1026a425a53f60a7b5e25902a53eb132", "start_type": "TTG", "rbs_motif": null, "genes": [ "frr" ], "truncated": "5-prime", "pseudogene": { "start": 541241, "stop": 541792, "observations": { "mutation": [ 541298 ], "stop": [ 541298 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47673", "query_cov": 1.0, "subject_cov": 0.7759562841530054, "identity": 0.993, "score": 264.0, "evalue": 1.68e-88, "gene_start": 1, "gene_end": 142, "reference_start": 42, "reference_end": 183, "reference_sequence": "MTKAHYIDFFKQAADKKIQWLKEELTKIRTGRPNPKIFDNLLIESYGQKMPLISLAQVTINPPREIIIKPFDPKSNTNAIYSEIQRANIGVQPVIDGEKIRVNFPQITQETRLENIKHVKKIIEQIYQELRVVRRDALQMIKKDNHNEDLENSLKAEIEKINKNYSNQLEEIQKDKEKELLTI" }, "paralog": false, "identity": 0.994535519125683, "score": 357.0, "evalue": 1.37e-127, "description": "Internal stop codon at 541298. Nonsense mutation around 541298.", "psc": { "uniref90_id": "UniRef90_P47673", "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_P47673", "cog_id": "COG0233", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47673", "UniRef:UniRef50_P47673", "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ] }, "pscc": { "uniref50_id": "UniRef50_P47673", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47673" ], "product": "Ribosome-recycling factor" } }, "id": "KKNAAMEMLJ_967", "locus": "GCA000027325_00967" }, { "type": "cds", "sequence": "contig_1", "start": 541789, "stop": 542094, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTGAACAACTCTCAAATCATCAGAGAACCAATTACCCCAACAATTAAAATAATGATTATGCTAAAGCTGAAACGAACAGCTTTAAAGTTTTCATTAATCTCAGTACTAATAACACTAGTAACACCACTTGCTTGTTCTACTTTTAATGTTCTTGAATTGAAATCTGCTGATCACGGTGATCAAAAGTTAAAACCATCAGCAAATGCGCTAATTAAAAGAAAAAAGCAAAATACAACTAATAAAGCAATGAATACTGAAGTTCGCTTTTTGATGAATTGTTTTGCTTTTTCATTCATTTAA", "aa": "MFVNNSQIIREPITPTIKIMIMLKLKRTALKFSLISVLITLVTPLACSTFNVLELKSADHGDQKLKPSANALIKRKKQNTTNKAMNTEVRFLMNCFAFSFI", "aa_hexdigest": "1f5a7a4795e76c8d92c36f3edb1f81c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11444.656099999982, "isoelectric_point": 10.24945774078369 }, "id": "KKNAAMEMLJ_968", "locus": "GCA000027325_00968" }, { "type": "cds", "sequence": "contig_1", "start": 542266, "stop": 542355, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGCAATAAAGCAAGCAATAGCTCCTAAGCTATATGAAAGTTGTAAGCTAAACTTAATTCTTGTTTTACTCATGCAAGCAATGTAA", "aa": "MLAIKQAIAPKLYESCKLNLILVLLMQAM", "aa_hexdigest": "fa9b394f8ba216dce46217d0ad3627f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3261.1224, "isoelectric_point": 9.102242851257323 }, "id": "KKNAAMEMLJ_969", "locus": "GCA000027325_00969" }, { "type": "cds", "sequence": "contig_1", "start": 542365, "stop": 542535, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCTAATGATGCCAAAAAAAGCTCCTTCTCATGTTTTTGATGGTGAAATTTTAATTAAAGGATTTTTACCAAAGCGCTTTCCAAAAAGAAAAGAAAATGTATCAACACTGACTGCTAAACTCATCAATAAAAAGATTGTAGTTCAACCCCTGATAACAGAAAAGTAA", "aa": "MVLMMPKKAPSHVFDGEILIKGFLPKRFPKRKENVSTLTAKLINKKIVVQPLITEK", "aa_hexdigest": "8633a74e7d51186d921461d7934546ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6402.811500000002, "isoelectric_point": 10.358860588073728 }, "id": "KKNAAMEMLJ_970", "locus": "GCA000027325_00970" }, { "type": "cds", "sequence": "contig_1", "start": 543066, "stop": 543581, "strand": "+", "frame": 3, "gene": "hsdS", "product": "Putative type I specificity subunit S-MgeORF438P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_Q49434" ], "nt": "TTGTTTGATTTAAAAAAAGGTGAAATGCTTGAAAAAGAGTTAATTACACCTGAGGGAAAATATGAATATTTTAACGGTGGTGTAAAAAATTCAGGAAGAACTGACAAGTTCAATACTTTTAAGAACACTATTAGTGTAATTGTTGGTGGATCTTGTGGTTATGTAAGGCTAGCTGATAAAAATTTCTTTTGTGGTCAAAGTAATTGCACATTAAATTTGTTAGATCCGCTTGAACTTGATCTTAAATTTGCTTACTATGCTTTGAAATCACAACAAGAAAGAATAGAGGCTTTAGCTTTTGGGACCACGATTCAAAACATTAGAATTTCAGATTTAAAAGAATTGGAAATTCCTTTCACATCAAATAAGAATGAACAGCATGCTATTGCAAATACTTTAAGTGTTTTTGATGAGAGACTAGAAAACTTAGCTTCTTTAATTGAGATTAACAGGAAACTAAGAGATGAATATGCTCACAAACTCTTTAGCTTAGATGAAGCTTTTCTAAGTCATTGA", "aa": "MFDLKKGEMLEKELITPEGKYEYFNGGVKNSGRTDKFNTFKNTISVIVGGSCGYVRLADKNFFCGQSNCTLNLLDPLELDLKFAYYALKSQQERIEALAFGTTIQNIRISDLKELEIPFTSNKNEQHAIANTLSVFDERLENLASLIEINRKLRDEYAHKLFSLDEAFLSH", "aa_hexdigest": "e5fdeeacefa01ea5afc6057b9fd7a38f", "start_type": "TTG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 543006, "stop": 543884, "observations": { "mutation": [ 543042, 543579 ], "stop": [ 543042, 543579 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49434", "query_cov": 1.0, "subject_cov": 0.4211822660098522, "identity": 0.9940000000000001, "score": 340.0, "evalue": 7.1e-115, "gene_start": 1, "gene_end": 171, "reference_start": 44, "reference_end": 214, "reference_sequence": "MGHHHHHHHHHHSSGHIDDDDKHMTPKLKLNNNINWTKRTIDSLFDLKKGEMLEKELITPEGKYEYFNGGVKNSGRTDKFNTFKNTISVIVGGSCGYVRLADKNFFCGQSNCTLNLLDPLELDLKFAYYALKSQQERIEALAFGTTIQNIRISDLKELEIPFTSNKNEQHAIANTLSVFDERLENLASLIEINRKLRDEYAHKLFSLDEAFLSHWKLEALQSQMHEITLGEIFNFKSGKYLKSEERLEEGKFPYYGAGIDNTGFVAEPNTEKDTISIISNGYSLGNIRYHEIPWFNGTGSIALEPMNNEIYVPFFYCALKYLQKDIKERMKSDDSPFLSLKLAGEIKVPYVKSFQLQRKAGKIVFLLDQKLDQYKKELSSLTVIRDTLLKKLFPDMTERTKSIKDY" }, "paralog": false, "identity": 0.9931506849315068, "score": 581.0, "evalue": 2.57e-212, "description": "Internal stop codon at 543042, 543579. Nonsense mutation around 543042, 543579.", "psc": { "uniref90_id": "UniRef90_Q49434", "gene": "HsdS", "product": "Putative type I specificity subunit S.MgeORF438P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49434", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "KKNAAMEMLJ_971", "locus": "GCA000027325_00971" }, { "type": "cds", "sequence": "contig_1", "start": 543606, "stop": 544157, "strand": "+", "frame": 3, "gene": "hsdS", "product": "Putative type I specificity subunit S-MgeORF438P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_Q49434" ], "nt": "ATGCATGAAATTACTTTAGGGGAAATATTTAATTTCAAAAGTGGTAAATATCTAAAGAGCGAGGAGAGATTAGAAGAAGGGAAATTTCCTTATTATGGAGCGGGAATTGATAACACTGGTTTTGTAGCTGAACCTAATACTGAAAAAGACACAATTTCTATTATTTCTAATGGTTATTCACTAGGTAACATCAGGTATCATGAAATTCCTTGGTTTAATGGCACAGGTAGCATTGCATTAGAACCCATGAATAATGAAATCTATGTACCATTCTTTTACTGTGCTTTAAAGTATTTGCAAAAAGATATTAAAGAAAGAATGAAAAGTGATGATTCACCTTTTTTATCCTTAAAACTAGCAGGTGAAATTAAAGTGCCTTATGTTAAGTCATTTCAACTGCAAAGAAAGGCAGGAAAAATCGTCTTTTTGTTAGATCAAAAATTAGACCAATATAAAAAAGAACTAAGTTCTTTAACAGTGATTCGTGACACTTTGTTAAAAAAATTATTCCCCGATATGACTGAAAGAACTAAATCTATTAAGGATTATTAA", "aa": "MHEITLGEIFNFKSGKYLKSEERLEEGKFPYYGAGIDNTGFVAEPNTEKDTISIISNGYSLGNIRYHEIPWFNGTGSIALEPMNNEIYVPFFYCALKYLQKDIKERMKSDDSPFLSLKLAGEIKVPYVKSFQLQRKAGKIVFLLDQKLDQYKKELSSLTVIRDTLLKKLFPDMTERTKSIKDY", "aa_hexdigest": "7d31abd2f905c0cba3c8bd336bca395d", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "5-prime", "pseudogene": { "start": 543306, "stop": 544157, "observations": { "mutation": [ 543579 ], "stop": [ 543579 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49434", "query_cov": 1.0, "subject_cov": 0.45073891625615764, "identity": 1.0, "score": 368.0, "evalue": 9.67e-126, "gene_start": 1, "gene_end": 183, "reference_start": 224, "reference_end": 406, "reference_sequence": "MGHHHHHHHHHHSSGHIDDDDKHMTPKLKLNNNINWTKRTIDSLFDLKKGEMLEKELITPEGKYEYFNGGVKNSGRTDKFNTFKNTISVIVGGSCGYVRLADKNFFCGQSNCTLNLLDPLELDLKFAYYALKSQQERIEALAFGTTIQNIRISDLKELEIPFTSNKNEQHAIANTLSVFDERLENLASLIEINRKLRDEYAHKLFSLDEAFLSHWKLEALQSQMHEITLGEIFNFKSGKYLKSEERLEEGKFPYYGAGIDNTGFVAEPNTEKDTISIISNGYSLGNIRYHEIPWFNGTGSIALEPMNNEIYVPFFYCALKYLQKDIKERMKSDDSPFLSLKLAGEIKVPYVKSFQLQRKAGKIVFLLDQKLDQYKKELSSLTVIRDTLLKKLFPDMTERTKSIKDY" }, "paralog": false, "identity": 0.9964664310954063, "score": 556.0, "evalue": 2.24e-202, "description": "Internal stop codon at 543579. Nonsense mutation around 543579.", "psc": { "uniref90_id": "UniRef90_Q49434", "gene": "HsdS", "product": "Putative type I specificity subunit S.MgeORF438P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49434", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "KKNAAMEMLJ_972", "locus": "GCA000027325_00972" }, { "type": "cds", "sequence": "contig_1", "start": 544562, "stop": 544915, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MG439", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P47677" ], "nt": "ATGACTGGTATAGTTGGTAGTGACAATACTAGTACTTCATTAATCAAATCCTTTTTAGCTAGTACAAGTGGTGTAAGTGAAAGTGATTTTACTGGTGATAAAGCTAATTTTGATGGTGATGTTATCTTTACTTATACTCCTCCAACAGATAATAGGAGGGTAAGTGAAAGTACTTTTAGTAGTATACCTGCTTCAATTAACTTTCCTTTTGATATCAAAATTGACATGAGTACTTCTCATGAAAAGTTGAATTTATTACTTTCAACAAATGAACAAGTGAAGAAAATTAGAACAAGAACTTTTAAGGGAAAGTCAATCGATCTTTTACCGTTTTTTTATTACACATTGCTTTAA", "aa": "MTGIVGSDNTSTSLIKSFLASTSGVSESDFTGDKANFDGDVIFTYTPPTDNRRVSESTFSSIPASINFPFDIKIDMSTSHEKLNLLLSTNEQVKKIRTRTFKGKSIDLLPFFYYTLL", "aa_hexdigest": "a03fffb2e085b6b3d2d4fdce297da6b4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 544562, "stop": 545215, "observations": { "mutation": [ 544957, 545173 ], "stop": [ 544957, 545173 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47677", "query_cov": 1.0, "subject_cov": 0.43014705882352944, "identity": 1.0, "score": 226.0, "evalue": 6.37e-73, "gene_start": 1, "gene_end": 117, "reference_start": 156, "reference_end": 272, "reference_sequence": "MKLRKIFLLPLISLSTLSVACSSTDPGLEKAQAYFQRNNIELSKNNAVTFLKKGYSSDKEEVINTVFASWKTTLLDYQLLEKPLDYSRFAKAFGVNKSKEDVTPNISAKGLYFDETYPGISGQIALVLGVKSQKVVNFQYSWKNNLDFKVQIHLKMTGIVGSDNTSTSLIKSFLASTSGVSESDFTGDKANFDGDVIFTYTPPTDNRRVSESTFSSIPASINFPFDIKIDMSTSHEKLNLLLSTNEQVKKIRTRTFKGKSIDLLPFFYYTLL" }, "paralog": false, "identity": 0.9907834101382489, "score": 419.0, "evalue": 2.9e-151, "description": "Internal stop codon at 544957, 545173. Nonsense mutation around 544957, 545173.", "psc": { "uniref90_id": "UniRef90_P47677", "product": "Uncharacterized lipoprotein MG439", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47677", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "KKNAAMEMLJ_973", "locus": "GCA000027325_00973" }, { "type": "cds", "sequence": "contig_1", "start": 545171, "stop": 545380, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAAACTCAGAAAGATCTTTTTATTACCATTAATTTCACTTAGTACTTTATCTGTAGCCTGTTCATCAACTGATCCTGGTTTAGAAAAAGCACAAGCTTATTTTCAGAGAAACAATATAGAACTTTCAAAAAATAATGCTGTTACTTTTTTAAAAAAGGGTTATAGTTCTGATAAGGAAGAGGTAATTAATACTGTATTTGCCAGTTGA", "aa": "MKLRKIFLLPLISLSTLSVACSSTDPGLEKAQAYFQRNNIELSKNNAVTFLKKGYSSDKEEVINTVFAS", "aa_hexdigest": "a6b45fd7970a198ce4bbfbb90c70e952", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.5507246376811594, "hmm_cov": 1.0, "evalue": 5.3421532672813134e-09, "score": 30.970706939697266, "start": 32, "stop": 69 } ], "seq_stats": { "molecular_weight": 7669.7820999999985, "isoelectric_point": 9.25103588104248 }, "id": "KKNAAMEMLJ_974", "locus": "GCA000027325_00974" }, { "type": "cds", "sequence": "contig_1", "start": 545380, "stop": 545952, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG440", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P47678" ], "nt": "TTGCAAGACGAAAAGATTCTTGAAAAACAAGACCCAAGTAGATTTGTAAAAGCATTTGGCAGTGGTAAGAGTAAGGAAGATGTTGAACCGAGCACTGGTGTTAAAGGCTTACGTTTAGTTGAAAGATACACGCAAAATGTTGCAAGTATTATTAATAATGTAATTAAATTGAGTAGTCAAACAGTTACAGATTTTAGTTTTTACTATAACAGTTCTCGTGATTTCAAAGTCCAAATAAGGTTACAAGCTACTGGAACTTTTGACAGTGCTCAAGCAAAATCATATTTGAGTCAAATAGGATTAAGTGATAGTGATATAAAAGATAAAAATTCTATTTCAGCTGATTTAATTTTTACTTATACTCCACCTTCCAGTAATTTATTTTCCAAATCTAGTTTTGATACTCTATTGAGAAAAATTAATTTTAATACCAATTTAAGGTTACAAATTATTGGTAAAGATGAATTGATGCAAAAGTTATTACAAAGTAATTTTGTAGGTCAATTAGCTGATCAAAACTTCCAAGATCAAACTATCGATCTTTTACCATATGTGCTTTATTCCATTTTGTAA", "aa": "MQDEKILEKQDPSRFVKAFGSGKSKEDVEPSTGVKGLRLVERYTQNVASIINNVIKLSSQTVTDFSFYYNSSRDFKVQIRLQATGTFDSAQAKSYLSQIGLSDSDIKDKNSISADLIFTYTPPSSNLFSKSSFDTLLRKINFNTNLRLQIIGKDELMQKLLQSNFVGQLADQNFQDQTIDLLPYVLYSIL", "aa_hexdigest": "aa48a3e9d5860d5cfb0b2536a55cf1cc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 545380, "stop": 546198, "observations": { "mutation": [ 545964, 546186 ], "stop": [ 545964, 546186 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47678", "query_cov": 1.0, "subject_cov": 0.6934306569343066, "identity": 0.995, "score": 359.0, "evalue": 4.59e-124, "gene_start": 1, "gene_end": 190, "reference_start": 85, "reference_end": 274, "reference_sequence": "MLKKQFWTKAGIISSLMVLGSLITACSNLSSGTNFFSLNQLRNSFSKNSELNQDKKELVTSLRDSFEVDSKSATNVLLDAWRFSLQDEKILEKQDPSRFVKAFGSGKSKEDVEPSTGVKGLRLVERYTQNVASIINNVIKLSSQTVTDFSFYYNSSRDFKVQIRLQATGTFDSAQAKSYLSQIGLSDSDIKDKNSISADLIFTYTPPSSNLFSKSSFDTLLRKINFNTNLRLQIIGKDELMQKLLQSNFVGQLADQNFQDQTIDLLPYVLYSIL" }, "paralog": false, "identity": 0.9889705882352942, "score": 510.0, "evalue": 4.37e-186, "description": "Internal stop codon at 545964, 546186. Nonsense mutation around 545964, 546186.", "psc": { "uniref90_id": "UniRef90_P47678", "product": "Uncharacterized protein MG440", "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47678", "UniRef:UniRef50_P47678" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" } }, "id": "KKNAAMEMLJ_975", "locus": "GCA000027325_00975" }, { "type": "cds", "sequence": "contig_1", "start": 545962, "stop": 546156, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGTATTAGGTTCTTTAATTACAGCATGCTCTAACTTAAGCAGTGGAACAAATTTTTCCAGTTTAAACCAACTTAGAAATAGTTTCAGTAAAAATAGTGAATTAAATCAAGACAAGAAAGAGTTAGTTACTTCACTAAGAGATAGTTTTGAAGTGGATTCAAAAAGTGCTACAAACGTTCTATTAGATGCTTGA", "aa": "MVLGSLITACSNLSSGTNFSSLNQLRNSFSKNSELNQDKKELVTSLRDSFEVDSKSATNVLLDA", "aa_hexdigest": "11ffeae36c3bc70ac7bf65898d4e2301", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.609375, "hmm_cov": 1.0, "evalue": 9.772673577846942e-11, "score": 36.658443450927734, "start": 26, "stop": 64 } ], "seq_stats": { "molecular_weight": 6926.618800000002, "isoelectric_point": 5.042550849914551 }, "id": "KKNAAMEMLJ_976", "locus": "GCA000027325_00976" }, { "type": "cds", "sequence": "contig_1", "start": 546515, "stop": 546709, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG441", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47679", "UniRef:UniRef90_P47679" ], "nt": "ATGAGTGTTAGTTTTCTTAGATCTAAATTTAGTTTAAAAGCATCAGTATTTGCATTTTTTGTACTGTTTCTTTTTTGTTTAAAAATAATATTAGTTTTATTTAGAAACTTTGGAAAAAGATTTAAGCATTTTTTGTTTAATCAGACTTCTTTATATCTTTTAGTTAGACTTTTTCAAAAAACAGAAATAGTTTGA", "aa": "MSVSFLRSKFSLKASVFAFFVLFLFCLKIILVLFRNFGKRFKHFLFNQTSLYLLVRLFQKTEIV", "aa_hexdigest": "d920382a78db0e86c7695572178ea31b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 546302, "stop": 546709, "observations": { "mutation": [ 546517 ], "stop": [ 546517 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47679", "query_cov": 1.0, "subject_cov": 0.47058823529411764, "identity": 1.0, "score": 115.0, "evalue": 8.95e-32, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MSVSFLRSKFSLKASVFAFFVLFLFCLKIILVLFRNFGKRFKHFLFNQTSLYLLVRLFQKTEIVWNLIANIHFFIKTQIQNLGIRLSRESISNETFQAVKLFHVNNLGLQEQEVINSKLSDYFCFFKYRNLLFVNW" }, "paralog": false, "identity": 0.9925925925925926, "score": 262.0, "evalue": 3.9e-92, "description": "Internal stop codon at 546517. Nonsense mutation around 546517.", "psc": { "uniref90_id": "UniRef90_P47679", "product": "Uncharacterized protein MG441", "uniref50_id": "UniRef50_P47679", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47679", "UniRef:UniRef50_P47679", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47679", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47679" ], "product": "Uncharacterized protein MG441" } }, "id": "KKNAAMEMLJ_977", "locus": "GCA000027325_00977" }, { "type": "cds", "sequence": "contig_1", "start": 546880, "stop": 547692, "strand": "-", "frame": 3, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254", "SO:0001217", "UniParc:UPI00001397F8", "UniRef:UniRef100_Q49435", "UniRef:UniRef50_P75135", "UniRef:UniRef90_Q49435" ], "nt": "ATGGACACATATACCTCCGCCAAAATTAATTGGTTCCCGGGTCACATGAAAAAGATCCATGATCAATTAAAAAAGTTATCTAGTCAAATTGATGGCATCATTGAGATAGTAGATGCTAGAGCACCAACTTTAACTCATAATTCAGAAATAATTAGTTATTTTTTAAATAAACCAAAATTAATACTGGCATTAAAGACAGATTTAGCACAATACAAACCAAATAAAAAGATCTTATTTGGTTCATTAAAAGAACCTTTTAAACTCAAAAAAAAAGTTCTAAAGACATTAACAACTTTATTTGCAAATAAAAGACAACAATTAAAAGCAAAAGGCTTATTAATCAAACAATTTAGATTAGCAGTTATTGGCATGCCCAATGTAGGTAAATCTAGTTTAATTAACCTTTTGATAAATAAAAATCACTTAAAAGTAGCTAACCGCGCTGGTATTACTAAATCATTAAATTGGATTCAAATTAGCCCTGAATTATTATTAAGTGATACCCCAGGAGTTTTTTTGAAAAGAATTGATGAAATTCAAATTGGATATAAACTAGTTTTGACTAATGTTATTAGAAGAGAAGTAGTTAATATAGAAGAAGTGGGAATGTTTGCTTTTAATTATCTTAAGAAACATTACAAACAGCTTTTACCATTTGAAGCGGATAGCTTTATTAACTTTTTGGAAAAATTTGCTAAAGTGAGAGGCTTAATTAAGAAAGCTAATGAACTAAACACTAACTTAGCATGTGAAATTTTTATTAATGAACTTATTAATGGGAAATATGGCAAATTAAGTTATGAACTTAATTAG", "aa": "MDTYTSAKINWFPGHMKKIHDQLKKLSSQIDGIIEIVDARAPTLTHNSEIISYFLNKPKLILALKTDLAQYKPNKKILFGSLKEPFKLKKKVLKTLTTLFANKRQQLKAKGLLIKQFRLAVIGMPNVGKSSLINLLINKNHLKVANRAGITKSLNWIQISPELLLSDTPGVFLKRIDEIQIGYKLVLTNVIRREVVNIEEVGMFAFNYLKKHYKQLLPFEADSFINFLEKFAKVRGLIKKANELNTNLACEIFINELINGKYGKLSYELN", "aa_hexdigest": "acc8691ae58f6c46ed2f9284e43966b1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001397F8", "uniref100_id": "UniRef100_Q49435", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001397F8", "UniRef:UniRef100_Q49435" ] }, "ips": { "uniref100_id": "UniRef100_Q49435", "uniref90_id": "UniRef90_Q49435" }, "psc": { "uniref90_id": "UniRef90_Q49435", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_P75135", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75135", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75135" ], "product": "Probable ribosome biogenesis GTPase A" }, "genes": [ "ylqF" ], "id": "KKNAAMEMLJ_978", "locus": "GCA000027325_00978" }, { "type": "cds", "sequence": "contig_1", "start": 547664, "stop": 548056, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "TTGCAAGATGTCAATAATTATCGTGATTCTGCTTGGGAAGTTAGTTTATTTTTCTCACCTAATTTAATTGCAACTTTTTTTTCAATTTTGTTAACAGGAACAGTAGTTAGTTATCTTTTCCCTCGTTATAATTTTGCTGAAATTAAAGTATTTACTGATAAGCTTGAAGAAGTTAGAAAAGCATTGTTAAGTGATAATGCTAATCACAGTTTATCTATTCAAGAAACGCTTGGTGGTTATTCTTTACTCAAAAAGAAGATGATAGTTTCTGTTTCCATGTATGTAGAAATTCCCCATTTAATTAAGATTATTAGACAAATTGACAAGGATTGTTTGGTATCTATTACAAGAATCAGGGGAATAGATGGACACATATACCTCCGCCAAAATTAA", "aa": "MQDVNNYRDSAWEVSLFFSPNLIATFFSILLTGTVVSYLFPRYNFAEIKVFTDKLEEVRKALLSDNANHSLSIQETLGGYSLLKKKMIVSVSMYVEIPHLIKIIRQIDKDCLVSITRIRGIDGHIYLRQN", "aa_hexdigest": "20399d3d976eaedb0230aa5010c7455a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 47, "aa_cov": 0.36153846153846153, "hmm_cov": 1.0, "evalue": 5.117527664161779e-10, "score": 34.19164276123047, "start": 77, "stop": 123 } ], "seq_stats": { "molecular_weight": 14988.261899999989, "isoelectric_point": 8.7098237991333 }, "id": "KKNAAMEMLJ_979", "locus": "GCA000027325_00979" }, { "type": "cds", "sequence": "contig_1", "start": 548354, "stop": 548851, "strand": "-", "frame": 2, "gene": "yitT", "product": "putative protein MG443", "db_xrefs": [ "COG:COG1284", "COG:S", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47681", "UniRef:UniRef90_P47681" ], "nt": "ATGAAATTTTTTAACAACTTATTTAAAAAAGAATCAAAGATAACAGTCGCATCTGGTTCTAAACGCGTAAGGATTTCTAACTCTTTTTTGATGTTTAGCAATCTTTATGAGGCTAAAAAACCATTAAAGTATGTATTAGTTTACTTACTATCAATTATTAATGCTTTTCTTTTACTTATTTTTATTCAAAAAACAGGTTTATATAGCTTTGGAATTAGTTCTTTAACTCAAGGTTTTGCTAGATTAGTGTTTGTTTTGTTAAAAAGTTTTGATGAAACTCAAAGATTATTAATTTTTAACATCCTTTACTGGCTTTTATATGTCTTTATCAACATCCCTTTAATTATTTTTTCTTATAAAAAAATAGGTAAAAACTTTACTATTCTTTCAACCCATTTTGTTGTTGCATCTAACGTATTTGGTTTTTTAATCAGTATTATTCCAGGTTCTGATAATTTACCACCAATGTTAGCTTCTATAACAGATACTAACTTTTGA", "aa": "MKFFNNLFKKESKITVASGSKRVRISNSFLMFSNLYEAKKPLKYVLVYLLSIINAFLLLIFIQKTGLYSFGISSLTQGFARLVFVLLKSFDETQRLLIFNILYWLLYVFINIPLIIFSYKKIGKNFTILSTHFVVASNVFGFLISIIPGSDNLPPMLASITDTNF", "aa_hexdigest": "21a3f0cfa91d3ef15159bd66e9bc5049", "start_type": "ATG", "rbs_motif": null, "genes": [ "yitT" ], "truncated": "5-prime", "pseudogene": { "start": 548051, "stop": 548851, "observations": { "mutation": [ 548305, 548356 ], "stop": [ 548305, 548356 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47681", "query_cov": 1.0, "subject_cov": 0.4177215189873418, "identity": 1.0, "score": 308.0, "evalue": 1.51e-102, "gene_start": 1, "gene_end": 165, "reference_start": 1, "reference_end": 165, "reference_sequence": "MKFFNNLFKKESKITVASGSKRVRISNSFLMFSNLYEAKKPLKYVLVYLLSIINAFLLLIFIQKTGLYSFGISSLTQGFARLVFVLLKSFDETQRLLIFNILYWLLYVFINIPLIIFSYKKIGKNFTILSTHFVVASNVFGFLISIIPGSDNLPPMLASITDTNFWKAAKDLNQSAGFVPFLWSDTSQGNVIISTFIYAAIYGFYNGISVSLLYILGGSAGGADFLTQYYARKKNRSVGSILFYVNSFILIIAILIGSFVAGSLLLQDVNNYRDSAWEVSLFFSPNLIATFFSILLTGTVVSYLFPRYNFAEIKVFTDKLEEVRKALLSDNANHSLSIQETLGGYSLLKKKMIVSVSMYVEIPHLIKIIRQIDKDCLVSITRIRGIDGHIYLRQN" }, "paralog": false, "identity": 0.9924812030075187, "score": 504.0, "evalue": 2.55e-182, "description": "Internal stop codon at 548305, 548356. Nonsense mutation around 548305, 548356.", "psc": { "uniref90_id": "UniRef90_P47681", "gene": "YitT", "product": "Uncharacterized protein MG443", "uniref50_id": "UniRef50_P47681", "cog_id": "COG1284", "cog_category": "S", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47681", "UniRef:UniRef50_P47681", "COG:COG1284", "COG:S", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47681", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47681" ], "product": "Uncharacterized protein MG443" } }, "id": "KKNAAMEMLJ_980", "locus": "GCA000027325_00980" }, { "type": "cds", "sequence": "contig_1", "start": 548867, "stop": 549226, "strand": "-", "frame": 2, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_010869484.1", "SO:0001217", "UniParc:UPI0000133CB2", "UniRef:UniRef100_P47682", "UniRef:UniRef50_P47682", "UniRef:UniRef90_P47682" ], "nt": "ATGAAAAAAATAAATAAACAAGCATTAATTGATGCAGTAGAACAAAAACAGTTAAAGGAATATGTTCCTGAATTTGGAGCAGGAGATGAAGTTAATGTTGCTATTAAGTTACGTGAAAAAGAAAAAGTTCGAGTTCAAAACTTCACTGGAACTGTTTTAAGAAGAAGGGGAAGAGGGATTAGCGAAACTTTCATGGTAAGAAAAACCACTGATGGAATTCCTATTGAAAAAAACTTTCAAATCCACAACCCTAATATAGACATAGAAGTAAAACGCAGGGGTAAAGTAAGAAGAGCATACATCTCTTATATGCGTGAAAGATCTGGTAAGTCAGCAAAAATTAAAGAAAAAAAGTCTTAA", "aa": "MKKINKQALIDAVEQKQLKEYVPEFGAGDEVNVAIKLREKEKVRVQNFTGTVLRRRGRGISETFMVRKTTDGIPIEKNFQIHNPNIDIEVKRRGKVRRAYISYMRERSGKSAKIKEKKS", "aa_hexdigest": "69ed898e384468424d405a0c31417fa3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133CB2", "ncbi_nrp_id": "WP_010869484.1", "uniref100_id": "UniRef100_P47682", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133CB2", "RefSeq:WP_010869484.1", "UniRef:UniRef100_P47682" ] }, "ips": { "uniref100_id": "UniRef100_P47682", "uniref90_id": "UniRef90_P47682" }, "psc": { "uniref90_id": "UniRef90_P47682", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_P47682", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47682", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47682" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "KKNAAMEMLJ_981", "locus": "GCA000027325_00981" }, { "type": "cds", "sequence": "contig_1", "start": 549210, "stop": 549752, "strand": "-", "frame": 1, "gene": null, "product": "tRNA (guanine-N(1)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "nt": "ATGGCTTATGGTGGAGGAAGTGGCATGGTTTTAAAAGCTGAACCTATTATTAATTGTTTAAATTTTTATAAAGCCCCAAATTCTCATGTAGTTTTACTCTCCCCAGAAGGTGAACAATTTTCTCAGAATTGTGCTAAAAAACTTACAAAATACGAACACTTAATTTTGTTATCTGGTCACTATGAAGGTTTTGATCAAAGAATTTATAAATATATTGATCAAATTGTTTCTTTAGGTGATTTTGTTTTAAGTGGTGGGGAACTTGTAGCACTAAGTGTTATTGATGCTACTGTTAGATTAATTAAAGGAGTTATTAATGATCAGAGTCTTATTTGTGAATCATTCAATGATAATCTATTAGATTTTCCTGTTTATACAAGGCCATACGATTTAAAAGGCGATAAAGTTCCTGAAGTTTTACTTTCAGGAGATCACCAAAAGATTGAATCATTTCGTAAAGAACAGCAAATCTTAAAAACTGCAAAATACAGACCTGATTTATACAAAAAATACTTAGAAAATAAAAATGAAAAAAATAAATAA", "aa": "MAYGGGSGMVLKAEPIINCLNFYKAPNSHVVLLSPEGEQFSQNCAKKLTKYEHLILLSGHYEGFDQRIYKYIDQIVSLGDFVLSGGELVALSVIDATVRLIKGVINDQSLICESFNDNLLDFPVYTRPYDLKGDKVPEVLLSGDHQKIESFRKEQQILKTAKYRPDLYKKYLENKNEKNK", "aa_hexdigest": "d4452856f851b30e01abd183ade9b5d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A534NV99", "query_cov": 0.9444444444444444, "subject_cov": 0.821256038647343, "identity": 0.512, "score": 167.0, "evalue": 3.19e-49, "valid": false, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_B1GZ20", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_B1GZ20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [], "id": "KKNAAMEMLJ_982", "locus": "GCA000027325_00982" }, { "type": "cds", "sequence": "contig_1", "start": 549792, "stop": 549905, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAATCACTGTTTTAACACTTTTTGAAAACACTATTTGGCCTTACTTAAATAGTTCTATTATGTTACAAGCTCAAAAAGCAAATTTAGTTCAATTTGAAGTAGTAAATTGA", "aa": "MKITVLTLFENTIWPYLNSSIMLQAQKANLVQFEVVN", "aa_hexdigest": "1cc73688319b6ba21c945286dd1b2383", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4298.030700000001, "isoelectric_point": 5.897352409362793 }, "id": "KKNAAMEMLJ_983", "locus": "GCA000027325_00983" }, { "type": "cds", "sequence": "contig_1", "start": 550019, "stop": 550174, "strand": "-", "frame": 2, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627", "SO:0001217", "UniRef:UniRef50_P47684", "UniRef:UniRef90_P47684" ], "nt": "ATGCGGATGGGAAGAGTACACTATCCGCTTTATAGAATAGTAGCGGTTGATTCGCGAGTAAAGCGTAATGGAAAGTATATCGCTTTAATTGGACATCTAAATCCAGCTTTAAAGGAAAATAAGTGTAAATTAGATGAAACTGTTGCCTTAGATTGA", "aa": "MRMGRVHYPLYRIVAVDSRVKRNGKYIALIGHLNPALKENKCKLDETVALD", "aa_hexdigest": "b576cd2be2db58e53cd9ebf649b721c7", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpsP" ], "truncated": "5-prime", "pseudogene": { "start": 549905, "stop": 550192, "observations": { "mutation": [ 550021 ], "stop": [ 550021 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47684", "query_cov": 1.0, "subject_cov": 0.5368421052631579, "identity": 1.0, "score": 106.0, "evalue": 7.08e-29, "gene_start": 1, "gene_end": 51, "reference_start": 7, "reference_end": 57, "reference_sequence": "MVKIRLMRMGRVHYPLYRIVAVDSRVKRNGKYIALIGHLNPALKENKCKLDETVALDWLNKGAIPTDTVRSLFSESGLWKKFIESKNKKETSPKK" }, "paralog": false, "identity": 0.9789473684210527, "score": 187.0, "evalue": 1.86e-63, "description": "Internal stop codon at 550021. Nonsense mutation around 550021.", "psc": { "uniref90_id": "UniRef90_P47684", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_P47684", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47684", "UniRef:UniRef50_P47684", "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47684" ], "product": "Small ribosomal subunit protein bS16" } }, "id": "KKNAAMEMLJ_984", "locus": "GCA000027325_00984" }, { "type": "cds", "sequence": "contig_1", "start": 550803, "stop": 550940, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAACTGCAAATGCTGCCTTAGCAGGTTTAATTAATATTGCTTTTGGTCAAATTCACACTAATCTGTTTATGGATAAATATTTCGCTAATCCACCACAAAATAAGTTGGTCAAATTTATTGAAAAGTACTCATAA", "aa": "MLTANAALAGLINIAFGQIHTNLFMDKYFANPPQNKLVKFIEKYS", "aa_hexdigest": "9c40812b28e08467067f1c353f298c22", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5057.8851, "isoelectric_point": 9.30383548736572 }, "id": "KKNAAMEMLJ_985", "locus": "GCA000027325_00985" }, { "type": "cds", "sequence": "contig_1", "start": 551040, "stop": 551969, "strand": "-", "frame": 1, "gene": "norM", "product": "putative protein MG447", "db_xrefs": [ "COG:COG0534", "COG:V", "GO:0005886", "GO:0015297", "GO:0042910", "SO:0001217", "UniRef:UniRef50_P47685", "UniRef:UniRef90_P47685" ], "nt": "TTGGTTTTATCTTTTGCAAAAGAATGGTTGCATTATAATCTTGATCAATCAAGTTTTGAACAAAATTTTCAAGCTAATAGCTTCCAACAGTTTTTTAACAAAAAGGCTATTGATGTAGCATCTGAATATGTTTATATTTTGATTGGTCTTAACATCATTCCTATGCTTTCAAGGTTGTTTTTTTATTTAGCACAATCAGAGGGAAGACAACTTTTTATTGCAATTGTTCCGCCTATTGCTAATTTGATTAACATATTAATAGTTTTTTTATTAGTTAGATATTCTTCTTTAGGAGTAATAGGATCAGCTGTGGCTGGAATACTAGGATACTTAATTAATTTTTTAGCTTATATTATTTATCTAATTTATTTAAACAAAAGAAATCTTACTTATTTAACTTTTAAAACTATTAAATTAAACAAAATTGACTTTAATCTACTAGTTGTAGTTTCATTAATAGGAATGGCATCCTTTTTTAGAAATGGATCACTTTCTATTGTTACTACATTTTATGAGAGTTTTTTGGTTAATTTGACAAAAGCAACTACTGATAAAAATGATGTTTTTTATCTGACACTTTTAACTGGACCTATTGCTATTAGTAATTTAGCTTCAGCAGCTATTTTTGGTTTATTACAAGGAGTTAGAACAGTTTCATCATATAAGTTTGGACAAAAAAAATATGATGAAATTAAAAAAATTAACATTTATACAGTTATTATCTGTATATCGTTCGGTAGTTTAATCTATTTGTTAACAGCAGTTGCTTTTGGAAAACAGATCTTATCTAGTTTTTTTGATGTTTCAGATCAAAATTTGGATTTAGCAAATTACTTTTCATTAATAGTGCAAGCACAAGTTTTCTTTGTAGCCACAGGCGCTAATAGTCAACAATATTTTCAAAATACTAACAGAGTGCTTTATTCTTGA", "aa": "MVLSFAKEWLHYNLDQSSFEQNFQANSFQQFFNKKAIDVASEYVYILIGLNIIPMLSRLFFYLAQSEGRQLFIAIVPPIANLINILIVFLLVRYSSLGVIGSAVAGILGYLINFLAYIIYLIYLNKRNLTYLTFKTIKLNKIDFNLLVVVSLIGMASFFRNGSLSIVTTFYESFLVNLTKATTDKNDVFYLTLLTGPIAISNLASAAIFGLLQGVRTVSSYKFGQKKYDEIKKINIYTVIICISFGSLIYLLTAVAFGKQILSSFFDVSDQNLDLANYFSLIVQAQVFFVATGANSQQYFQNTNRVLYS", "aa_hexdigest": "da3c17d4ed64e215a038c41e3230e806", "start_type": "TTG", "rbs_motif": null, "genes": [ "norM" ], "truncated": "both", "pseudogene": { "start": 550803, "stop": 552269, "observations": { "mutation": [ 550943, 551042, 552020 ], "stop": [ 550943, 551042, 552020 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47685", "query_cov": 1.0, "subject_cov": 0.5648994515539305, "identity": 0.997, "score": 501.0, "evalue": 5.04e-174, "gene_start": 1, "gene_end": 309, "reference_start": 160, "reference_end": 468, "reference_sequence": "MNSKRDRFEKQLLIKNVFESKQLFLTILRFTVPTFFFALFSAAYVFIDQIMVIKFVPRSELNPDSIFTDQSLIDEFKNSAFYKSDSFLSSGLNIKQFIKTVLNISQPLIVLLNAINIFIPLGTGVIFSKAIGRNDQNKIQEAWNTGLISTTVFGLITQFLVLSFAKEWLHYNLDQSSFEQNFQANSFQQFFNKKAIDVASEYVYILIGLNIIPMLSRLFFYLAQSEGRQLFIAIVPPIANLINILIVFLLVRYSSLGVIGSAVAGILGYLINFLAYIIYLIYLNKRNLTYLTFKTIKLNKIDFNLLVVVSLIGMASFFRNGSLSIVTTFYESFLVNLTKATTDKNDVFYLTLLTGPIAISNLASAAIFGLLQGVRTVSSYKFGQKKYDEIKKINIYTVIICISFGSLIYLLTAVAFGKQILSSFFDVSDQNLDLANYFSLIVQAQVFFVATGANSQQYFQNTNRVLYSWIVSLTHGLFVFIPLLFIFQAITLQTNNIEVFIWLLTANAALAGLINIAFGQIHTNLFMDKYFANPPQNKLVKFIEKYS" }, "paralog": false, "identity": 0.9938524590163934, "score": 852.0, "evalue": 1.49e-314, "description": "Internal stop codon at 550943, 551042, 552020. Nonsense mutation around 550943, 551042, 552020.", "psc": { "uniref90_id": "UniRef90_P47685", "gene": "NorM", "product": "Uncharacterized protein MG447", "uniref50_id": "UniRef50_P47685", "cog_id": "COG0534", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015297", "GO:0042910" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47685", "UniRef:UniRef50_P47685", "COG:COG0534", "COG:V", "GO:0005886", "GO:0015297", "GO:0042910" ] }, "pscc": { "uniref50_id": "UniRef50_P47685", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47685" ], "product": "Uncharacterized protein MG447" } }, "id": "KKNAAMEMLJ_986", "locus": "GCA000027325_00986" }, { "type": "cds", "sequence": "contig_1", "start": 552018, "stop": 552446, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAGTAAAAGGGATCGTTTTGAAAAACAACTCCTAATTAAAAATGTTTTTGAATCAAAACAGCTTTTTCTAACTATTCTTAGATTTACTGTTCCAACTTTTTTCTTTGCACTTTTTAGTGCTGCTTATGTATTTATAGATCAAATCATGGTGATTAAGTTTGTTCCTAGAAGTGAACTTAATCCTGATTCTATTTTTACTGATCAGAGTTTAATTGATGAATTTAAAAATTCTGCTTTTTATAAAAGTGATAGTTTTTTAAGCTCTGGACTTAATATTAAACAATTTATTAAAACTGTTTTAAATATTAGTCAACCTTTGATTGTTTTGTTAAACGCCATTAATATTTTTATTCCTCTAGGAACTGGAGTTATTTTTTCTAAAGCAATTGGTAGAAATGATCAAAATAAAATTCAGGAAGCTTGA", "aa": "MNSKRDRFEKQLLIKNVFESKQLFLTILRFTVPTFFFALFSAAYVFIDQIMVIKFVPRSELNPDSIFTDQSLIDEFKNSAFYKSDSFLSSGLNIKQFIKTVLNISQPLIVLLNAINIFIPLGTGVIFSKAIGRNDQNKIQEA", "aa_hexdigest": "6abbd272c12a14bf8db4d667cd274ee9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16280.81949999998, "isoelectric_point": 9.474934577941895 }, "id": "KKNAAMEMLJ_987", "locus": "GCA000027325_00987" }, { "type": "cds", "sequence": "contig_1", "start": 552447, "stop": 552632, "strand": "-", "frame": 1, "gene": null, "product": "peptide-methionine (R)-S-oxide reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU5ZP34" ], "nt": "ATGATAAGAACTGAAGTTCGTGCTAAAAACAGTGATAGCCACTTGGGACATGTTTTTAATGATGGACCGAGTGAATTAGGTGGATTGCGCTATTGTATTAATTCTGCAGCTTTAAAATTTATTCCTTTTGAAGAGATGGAAAAATTAGGATACAAGGATTACATCCATCTTTTTAAAAATAAATAA", "aa": "MIRTEVRAKNSDSHLGHVFNDGPSELGGLRYCINSAALKFIPFEEMEKLGYKDYIHLFKNK", "aa_hexdigest": "cb3735859ffda3bb46315f0f2a15d0ca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAU5ZP34", "query_cov": 0.9508196721311475, "subject_cov": 0.8656716417910447, "identity": 0.638, "score": 86.3, "evalue": 4.27e-21, "valid": false, "product": "peptide-methionine (R)-S-oxide reductase", "uniref50_id": "UniRef50_A0AAU5ZP34" }, "pscc": { "uniref50_id": "UniRef50_A0AAU5ZP34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU5ZP34" ], "product": "peptide-methionine (R)-S-oxide reductase" }, "genes": [], "id": "KKNAAMEMLJ_988", "locus": "GCA000027325_00988" }, { "type": "cds", "sequence": "contig_1", "start": 552696, "stop": 552899, "strand": "-", "frame": 1, "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "db_xrefs": [ "COG:COG0229", "COG:O", "EC:1.8.4.12", "GO:0006979", "GO:0030091", "GO:0033743", "SO:0001217", "UniRef:UniRef50_Q8E026", "UniRef:UniRef90_P47686" ], "nt": "ATGAGTAAATACCAAAAAAAGAGTGAAGCTGAGCTAAAACGTACTTTAACCAAACTCCAGTTTGATGTAACGCAAAATGCTCACACTGAGCCACCTTACATTAATGAATATAACCGCAATTTTGAAAAGGGAATTTATGTTGATATAACATCTGGTGAACCACTTTTTATTTCAACTGATAAATTTAAATCTGGTTGTGGTTGA", "aa": "MSKYQKKSEAELKRTLTKLQFDVTQNAHTEPPYINEYNRNFEKGIYVDITSGEPLFISTDKFKSGCG", "aa_hexdigest": "0f07e3d21a7b38b6e7204dc83e7933ea", "start_type": "ATG", "rbs_motif": null, "genes": [ "msrB" ], "truncated": "5-prime", "pseudogene": { "start": 552447, "stop": 552899, "observations": { "mutation": [ 552698 ], "stop": [ 552698 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47686", "query_cov": 1.0, "subject_cov": 0.44666666666666666, "identity": 1.0, "score": 139.0, "evalue": 4.02e-41, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MSKYQKKSEAELKRTLTKLQFDVTQNAHTEPPYINEYNRNFEKGIYVDITSGEPLFISTDKFKSGCGWPAFSKPIDKNLIANYRDESHGMIRTEVRAKNSDSHLGHVFNDGPSELGGLRYCINSAALKFIPFEEMEKLGYKDYIHLFKNK" }, "paralog": false, "identity": 0.9933333333333333, "score": 308.0, "evalue": 5.55e-110, "description": "Internal stop codon at 552698. Nonsense mutation around 552698.", "psc": { "uniref90_id": "UniRef90_P47686", "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "ec_ids": [ "1.8.4.12" ], "uniref50_id": "UniRef50_Q8E026", "cog_id": "COG0229", "cog_category": "O", "go_ids": [ "GO:0033743", "GO:0030091", "GO:0006979" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47686", "EC:1.8.4.12", "UniRef:UniRef50_Q8E026", "COG:COG0229", "COG:O", "GO:0033743", "GO:0030091", "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_Q8E026", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8E026" ], "product": "Peptide methionine sulfoxide reductase MsrB" } }, "id": "KKNAAMEMLJ_989", "locus": "GCA000027325_00989" }, { "type": "cds", "sequence": "contig_1", "start": 552896, "stop": 553297, "strand": "-", "frame": 2, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "db_xrefs": [ "GO:0000049", "SO:0001217", "UniRef:UniRef50_P47687", "UniRef:UniRef90_P47687" ], "nt": "ATGAAAAGAATAAGTGAGCTTTTAGGTTATGATCTTATTTCATTAGCAAATAAAGTTCCTTTTGTTGTTTGTGAAGTTGTTTCAGTTATTCCTATTGCAAACACCCATCTAAAAAGGTGTAAAGTTAACACTGGTTTGACAAAAAGCTTGGATATTGTTTGTGGAGCTAATAATGTTAGAGTTGGTATGAAAACAGTTTTAGTACAAGTAGGTGGAGTGTTACCTAGCGGTACAGTTATCAAAAAAACTAAAATAGCTGGTTTTGATTCAGTTGGCATGTTATGTTCTGAAAAAGAACTTAATTTAAAACAGAAAAATGAGGGTATAATTGAGATTAATCCTAAGATCAAAGTAGGAAAACCATTTATTGATGTATATTTAAATAAACAACATTCCAAATGA", "aa": "MKRISELLGYDLISLANKVPFVVCEVVSVIPIANTHLKRCKVNTGLTKSLDIVCGANNVRVGMKTVLVQVGGVLPSGTVIKKTKIAGFDSVGMLCSEKELNLKQKNEGIIEINPKIKVGKPFIDVYLNKQHSK", "aa_hexdigest": "d4902a162519a9d81a112745d4fbe60f", "start_type": "ATG", "rbs_motif": null, "genes": [ "ytpR" ], "truncated": "both", "pseudogene": { "start": 552863, "stop": 553548, "observations": { "deletion": [ 553347 ], "mutation": [ 553425 ], "stop": [ 552897, 553425 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47687", "query_cov": 1.0, "subject_cov": 0.5833333333333334, "identity": 1.0, "score": 258.0, "evalue": 1.44e-85, "gene_start": 1, "gene_end": 133, "reference_start": 85, "reference_end": 217, "reference_sequence": "MFDISKDFVSIFYRKETLKNCMFGIIGSRSETTLRQQNDKDWSFFVNDANRITGFNFFNIKKSFKKFFLSHRFNEGLNYPSLKIMKRISELLGYDLISLANKVPFVVCEVVSVIPIANTHLKRCKVNTGLTKSLDIVCGANNVRVGMKTVLVQVGGVLPSGTVIKKTKIAGFDSVGMLCSEKELNLKQKNEGIIEINPKIKVGKPFIDVYLNKQHSKWVNTKKRVKLS" }, "paralog": false, "identity": 0.9912280701754386, "score": 440.0, "evalue": 4.68e-160, "description": "Internal stop codon at 552897, 553425. Frameshift due to deletion around 553347. Nonsense mutation around 553425.", "psc": { "uniref90_id": "UniRef90_P47687", "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "uniref50_id": "UniRef50_P47687", "go_ids": [ "GO:0000049" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47687", "UniRef:UniRef50_P47687", "GO:0000049" ] }, "pscc": { "uniref50_id": "UniRef50_P47687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47687" ], "product": "Uncharacterized protein MG449" } }, "id": "KKNAAMEMLJ_990", "locus": "GCA000027325_00990" }, { "type": "cds", "sequence": "contig_1", "start": 553423, "stop": 553548, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGATATATCTAAAGATTTTGTTTCTATCTTTTATCGTAAAGAAACATTAAAAAACTGTATGTTTGGCATCATAGGAAGTAGAAGTGAAACAACTTTACGCCAACAAAATGACAAAGACTGA", "aa": "MFDISKDFVSIFYRKETLKNCMFGIIGSRSETTLRQQNDKD", "aa_hexdigest": "0beeec6f9cc5fde636670048409328d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4863.506200000002, "isoelectric_point": 7.900616645812989 }, "id": "KKNAAMEMLJ_991", "locus": "GCA000027325_00991" }, { "type": "cds", "sequence": "contig_1", "start": 553558, "stop": 553827, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAGTTTTTTAACAAAGCACAATCTGCTAGCAGTGCTCATAAGTTAGCAGTTCAATTTTTAGAAAAAAAGCTATTAAAAAAAGAAGTTAATTTCAGAAGAGCGGCTTTGTTGACAACAATGTTTGAAACTGATAAAAATCAACTCATAAAGCAAGAATTTGTTACGCTTCTCAACAATGCAGTTGATGTAAGTGAGCACTTGCTTTCACCTGTAATTTGTACACACACAGGTATCAATAGTTATGCCTTTTTAATACAAACAGAATAA", "aa": "MQFFNKAQSASSAHKLAVQFLEKKLLKKEVNFRRAALLTTMFETDKNQLIKQEFVTLLNNAVDVSEHLLSPVICTHTGINSYAFLIQTE", "aa_hexdigest": "30e4fe475650da5e6f639ddfeeb58777", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10142.645699999992, "isoelectric_point": 8.853910636901855 }, "id": "KKNAAMEMLJ_992", "locus": "GCA000027325_00992" }, { "type": "cds", "sequence": "contig_1", "start": 554122, "stop": 554430, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGATCGCTTTTTTAGTTGATTCTGTCAGTAATCTAAAGGAAGATAAAAATAGTCATCTCTATGTTTTACCGCTTTATATTATTGAAACAATTGCTGAACACCAAGAAACATTCAAATCAGGTTTTAACATTGATCTTAAAACCTTAACTGATAAGATGATTAACGCCCCTAAAGGTGTTAAGTTTTCAACATCACAAACTTCTGAAGAAGAGGTTCGTGACAAAGTTAAGAGCATAATTAATGATTATGATCTTATCATAGGAATCCCTATTGATAAAGAGATCTCAACCTCCTATTTAAATTGA", "aa": "MRIAFLVDSVSNLKEDKNSHLYVLPLYIIETIAEHQETFKSGFNIDLKTLTDKMINAPKGVKFSTSQTSEEEVRDKVKSIINDYDLIIGIPIDKEISTSYLN", "aa_hexdigest": "f28183294358239411cc3406fbfb9a8f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11623.14019999999, "isoelectric_point": 5.078984642028807 }, "id": "KKNAAMEMLJ_993", "locus": "GCA000027325_00993" }, { "type": "cds", "sequence": "contig_1", "start": 554430, "stop": 555614, "strand": "-", "frame": 1, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "COG:COG0050", "COG:J", "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020", "RefSeq:WP_009885583.1", "SO:0001217", "UniParc:UPI0000129DB5", "UniRef:UniRef100_P13927", "UniRef:UniRef50_P23568", "UniRef:UniRef90_P23568" ], "nt": "ATGGCAAGAGAGAAATTTGACCGTTCCAAACCACATGTCAATGTTGGTACCATTGGTCACATTGACCATGGTAAAACCACTTTAACAGCTGCTATCTGTACAGTTTTAGCAAAGGAAGGAAAATCAGCTGCAACGCGTTATGATGAAATTGATAAAGCCCCTGAAGAAAAAGCAAGGGGAATCACAATTAACTCTGCACACGTAGAATATTCTTCTGACAAACGTCACTATGCCCATGTTGACTGTCCTGGACATGCTGACTACATTAAAAATATGATCACAGGTGCTGCACAAATGGATGGAGCTATTCTAGTTGTTTCAGCAACTGATAGTGTGATGCCCCAAACCCGCGAGCACATCTTACTTGCCCGCCAAGTAGGGGTTCCTAAAATGGTAGTTTTTCTAAACAAGTGTGATATTGCTAGTGATGAAGAGGTACAAGAACTTGTTGCTGAAGAAGTACGTGATCTGTTAACTTCCTATGGTTTTGATGGTAAGAACACTCCTATTATTTATGGCTCAGCTTTAAAAGCATTGGAAGGTGATCCAAAGTGGGAGGCTAAGATCCATGATTTGATTAAAGCAGTTGATGAATGGATTCCAACTCCTACACGTGAAGTAGATAAACCTTTCTTATTAGCAATTGAAGATACGATGACCATTACTGGTAGAGGTACAGTTGTTACAGGAAGAGTTGAAAGAGGTGAACTCAAAGTAGGTCAAGAAGTTGAAATTGTTGGTTTAAAACCAATTAGAAAAGCAGTTGTTACTGGAATTGAAATGTTCAAAAAGGAACTTGATTCAGCAATGGCTGGTGACAATGCTGGGGTATTATTACGTGGTGTTGAACGTAAAGAAGTTGAAAGAGGTCAAGTTTTAGCAAAACCAGGCTCTATTAAACCGCACAAGAAATTTAAAGCTGAGATCTATGCTTTAAAGAAAGAAGAAGGTGGTAGACACACTGGTTTTTTAAACGGTTACCGTCCTCAATTCTATTTCCGTACCACTGATGTAACTGGTTCTATTGCTTTAGCTGAAAATACTGAAATGGTTCTACCTGGTGATAATGCTTCTATTACTGTTGAGTTAATTGCTCCTATCGCTTGTGAAAAAGGTAGTAAGTTCTCAATTCGTGAAGGTGGTAGAACTGTAGGGGCAGGCACTGTAACAGAAGTTCTAGAATAG", "aa": "MAREKFDRSKPHVNVGTIGHIDHGKTTLTAAICTVLAKEGKSAATRYDEIDKAPEEKARGITINSAHVEYSSDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSATDSVMPQTREHILLARQVGVPKMVVFLNKCDIASDEEVQELVAEEVRDLLTSYGFDGKNTPIIYGSALKALEGDPKWEAKIHDLIKAVDEWIPTPTREVDKPFLLAIEDTMTITGRGTVVTGRVERGELKVGQEVEIVGLKPIRKAVVTGIEMFKKELDSAMAGDNAGVLLRGVERKEVERGQVLAKPGSIKPHKKFKAEIYALKKEEGGRHTGFLNGYRPQFYFRTTDVTGSIALAENTEMVLPGDNASITVELIAPIACEKGSKFSIREGGRTVGAGTVTEVLE", "aa_hexdigest": "61ef27a09c235dba715d0dea52d18464", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129DB5", "ncbi_nrp_id": "WP_009885583.1", "uniref100_id": "UniRef100_P13927", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129DB5", "RefSeq:WP_009885583.1", "UniRef:UniRef100_P13927" ] }, "ips": { "uniref100_id": "UniRef100_P13927", "uniref90_id": "UniRef90_P23568" }, "psc": { "uniref90_id": "UniRef90_P23568", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_P23568", "cog_id": "COG0050", "cog_category": "J", "go_ids": [ "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P23568", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P23568" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "KKNAAMEMLJ_994", "locus": "GCA000027325_00994" }, { "type": "cds", "sequence": "contig_1", "start": 555784, "stop": 555933, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCACTAGTGCTTTTGTTTTAACTGGAATTGTTGAAAGTCTTTTAACATTTGGAACTATTATTGAACAAATTGATAAATTCACTGATCAGACTAATGTGATGTTATTAATTTATGCAGTTATCTACACTTTTAATCCAAAAAGTTGA", "aa": "MVTSAFVLTGIVESLLTFGTIIEQIDKFTDQTNVMLLIYAVIYTFNPKS", "aa_hexdigest": "78fe6aba90e9a10c824b534ff9edf733", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5487.3813, "isoelectric_point": 4.316091728210449 }, "id": "KKNAAMEMLJ_995", "locus": "GCA000027325_00995" }, { "type": "cds", "sequence": "contig_1", "start": 555934, "stop": 556311, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG452", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75125", "UniRef:UniRef90_P47690" ], "nt": "TTGTTAAAAAACCAACAATTCTTTTTAAGTGCATTAGCTTATATATTATTTACTTTTATTGGCTATAACCTAATTTTGTCAATAGCTGGTATAGCTTATAAATCAACAAATCCATATAAGTTAACAAGTAGTATTTTTCTCCATGTAATTGCACCAATAGCATTCTTCATAGCAAGTTTTATCAAAATAAAACATGAGAAAGATGTCAATATTAACATGTTCTTTAAAAGCCTATTATTATTCATGATCTATCCTTTAATATATGGGCTTTATTTAGTAACTATTCCATATGTAAGGCATTATCTTTTTAATGGTAGGCCATCTACTTATACCATTTATGGCAGCATTACAAATACTAAAAATAATCCTTTTGCTTGA", "aa": "MLKNQQFFLSALAYILFTFIGYNLILSIAGIAYKSTNPYKLTSSIFLHVIAPIAFFIASFIKIKHEKDVNINMFFKSLLLFMIYPLIYGLYLVTIPYVRHYLFNGRPSTYTIYGSITNTKNNPFA", "aa_hexdigest": "e15510d147283391b09147e91d31a9e7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 555667, "stop": 556452, "observations": { "mutation": [ 555727, 555730, 555931, 556309, 556438 ], "stop": [ 555727, 555730, 555931, 556309, 556438 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47690", "query_cov": 1.0, "subject_cov": 0.4789272030651341, "identity": 0.992, "score": 239.0, "evalue": 8.65e-78, "gene_start": 1, "gene_end": 125, "reference_start": 90, "reference_end": 214, "reference_sequence": "MLIIFKDLNLVKTNNCQIIGWWKQLSLPQKTFLSFIPLFLVTSAFVLTGIVESLLTFGTIIEQIDKFTDQTNVMLLIYAVIYTFNPKSWLLKNQQFFLSALAYILFTFIGYNLILSIAGIAYKSTNPYKLTSSIFLHVIAPIAFFIASFIKIKHEKDVNINMFFKSLLLFMIYPLIYGLYLVTIPYVRHYLFNGRPSTYTIYGSITNTKNNPFAWLVVFAVLFIYFPLSYLAIYLLQLKLIKKAIQPQFNLPFTLNKWKQK" }, "paralog": false, "identity": 0.9808429118773946, "score": 494.0, "evalue": 7.73e-181, "description": "Internal stop codon at 555727, 555730, 555931, 556309, 556438. Nonsense mutation around 555727, 555730, 555931, 556309, 556438.", "psc": { "uniref90_id": "UniRef90_P47690", "product": "Uncharacterized protein MG452", "uniref50_id": "UniRef50_P75125", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47690", "UniRef:UniRef50_P75125", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75125", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75125" ], "product": "Uncharacterized protein MG452 homolog" } }, "id": "KKNAAMEMLJ_996", "locus": "GCA000027325_00996" }, { "type": "cds", "sequence": "contig_1", "start": 556437, "stop": 557315, "strand": "+", "frame": 3, "gene": "galU", "product": "UTP--glucose-1-phosphate uridylyltransferase GalU", "db_xrefs": [ "COG:COG1210", "COG:M", "EC:2.7.7.9", "GO:0003983", "GO:0006011", "GO:0009058", "RefSeq:WP_009885581.1", "SO:0001217", "UniParc:UPI0000053FBB", "UniRef:UniRef100_P47691", "UniRef:UniRef50_P47691", "UniRef:UniRef90_P47691" ], "nt": "ATGAAAACAAAAATAAGAAAAGCAGTTATTCCTGCTGCTGGGTTGGGTGTTAGGTTACTACCAGCAACAAAAGCAATTCCCAAAGAGATGTTACCATTGGTAAATAAACCTACTATCCAATACATAGTAGAGGAAGCAGTTAAAAGTGGCATTGAACAGATTCTTGTCATTGTTTCATCCAAAAAAACAGCTATATTAGATCATTTTGATTATGATCTGATCTTAGAAAATGCCTTAATTCAAAAAAATAAATTGCAGGAGCATAAAGAGATTGAAGATATTGCTAATTTAGCACATATCTTTTTTGTTAGACAAAAAAATCAAGATGGTTTGGGAGATGCAATCTTGTTTGCTGAATCTTTTGTTGGTAATGAAGACTTTGCAGTATTGTTAGGTGATGATGTTGTTTTTAGTAAAGAACCTGCTTTAAAACAATGCTTGGAAGCTTATTATGAAACTAATTGTCAAACAATCGGTGTACAAGAAGTAGATCCTTGTCATGTTGATAAGTATGGAATTATCACCCCTGAAGGTGATTACAAAAATAAAGATCTTATTAAGGTTTTAGCAATGACTGAAAAACCTAAACCAAAAGATGCTAAAAGTAATTTAGCAATCTTAGGGCGATATGTACTCAAACCATCTATTTTCAAAGCACTTAGAAGTGTACCTTATGGAGTTGGTGGTGAGTTGCAACTAACTGATGGTTTAAATTTTTGTTTGAAAAATGAAAACTTTTATGCAAGAAAGTTTACTGGTACTAGGTTTGATGTTGGCACAAAGAGTGGTTTTATTAAAGCAAATTTATTTACTGCTTTAAACAATAAAGATATTAGTAAAAAAGAAGTTTTAGAACTTTTAAATTTAGTTAAAGCTTAA", "aa": "MKTKIRKAVIPAAGLGVRLLPATKAIPKEMLPLVNKPTIQYIVEEAVKSGIEQILVIVSSKKTAILDHFDYDLILENALIQKNKLQEHKEIEDIANLAHIFFVRQKNQDGLGDAILFAESFVGNEDFAVLLGDDVVFSKEPALKQCLEAYYETNCQTIGVQEVDPCHVDKYGIITPEGDYKNKDLIKVLAMTEKPKPKDAKSNLAILGRYVLKPSIFKALRSVPYGVGGELQLTDGLNFCLKNENFYARKFTGTRFDVGTKSGFIKANLFTALNNKDISKKEVLELLNLVKA", "aa_hexdigest": "1107b311c79eb86ee11611e83b4f1807", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000053FBB", "ncbi_nrp_id": "WP_009885581.1", "uniref100_id": "UniRef100_P47691", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000053FBB", "RefSeq:WP_009885581.1", "UniRef:UniRef100_P47691" ] }, "ips": { "uniref100_id": "UniRef100_P47691", "uniref90_id": "UniRef90_P47691" }, "psc": { "uniref90_id": "UniRef90_P47691", "gene": "galU", "product": "UTP--glucose-1-phosphate uridylyltransferase GalU", "ec_ids": [ "2.7.7.9" ], "uniref50_id": "UniRef50_P47691", "cog_id": "COG1210", "cog_category": "M", "go_ids": [ "GO:0003983", "GO:0006011", "GO:0009058" ] }, "pscc": { "uniref50_id": "UniRef50_P47691", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47691" ], "product": "UTP--glucose-1-phosphate uridylyltransferase" }, "genes": [ "galU" ], "id": "KKNAAMEMLJ_997", "locus": "GCA000027325_00997" }, { "type": "cds", "sequence": "contig_1", "start": 557305, "stop": 557721, "strand": "-", "frame": 3, "gene": "osmC", "product": "Organic hydroperoxide resistance protein-like", "db_xrefs": [ "COG:COG1764", "COG:V", "EC:1.11.1.28", "GO:0006979", "KEGG:K04063", "SO:0001217", "UniRef:UniRef50_P47692", "UniRef:UniRef90_P47692" ], "nt": "TTGATTTATAAAACTGTTGCACAAACTGAAACTGGCAGAGAAGGTAGTGTTAAAACTTTAGATGGTTTTCAAACAAAACTTAGTTTTCCCAAACCTGATTTATCAGTTCAAACAGAAAATAATCCTGAGCAGTTATTTGCATCTGCTTATGCAAGTTGTTTTTCTCAAGCAGTAATTGTTGTTATGCAACAACATCAATTTAGTTTTTCAAAAAAACCAGTTGTAAGTGTTAAAGTAGAACTCCATCAAGAAAATGGACTATTTCACATTAAGGCTGGTGTTGAATTAACTACTAATAGTAATGACCAAGAAGTTGGTAAAAAACTAATTCAAAAAGCCCATGAAATGTGTCCTTTTAGTCGATTGATTAGAAATGAAAACTTCCTTGGTTTAACTTTAAATGGTATTAAGCTTTAA", "aa": "MIYKTVAQTETGREGSVKTLDGFQTKLSFPKPDLSVQTENNPEQLFASAYASCFSQAVIVVMQQHQFSFSKKPVVSVKVELHQENGLFHIKAGVELTTNSNDQEVGKKLIQKAHEMCPFSRLIRNENFLGLTLNGIKL", "aa_hexdigest": "61c01b224f98155a3aea4c6d3e4fbb5e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47692", "query_cov": 1.0, "subject_cov": 0.9857142857142858, "identity": 0.993, "score": 273.0, "evalue": 5.1e-93, "valid": true, "gene": "OsmC", "product": "Organic hydroperoxide resistance protein-like", "ec_ids": [ "1.11.1.28" ], "uniref50_id": "UniRef50_P47692", "cog_id": "COG1764", "cog_category": "V", "kegg_orthology_id": "K04063", "go_ids": [ "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_P47692", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47692" ], "product": "Organic hydroperoxide resistance protein-like" }, "genes": [ "osmC" ], "id": "KKNAAMEMLJ_998", "locus": "GCA000027325_00998" }, { "type": "cds", "sequence": "contig_1", "start": 557726, "stop": 558124, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTAATATAGATAATATTTTAGTAAAAATGTTAAATAACATATTGCAATTTCTCAAAGAAAGAGAACTTTATTCACAAGCTAATTTTGAAACAGAACTAGATAACCATTTAAAAGAGAAAAAAAATAACTTTTATGTTGGTTTTGATCCAACTGCTAATTCTTTACATATTGGCAATTATGTTTTAATTCACATTGCAAAATTATTAAAAGACATGGGGCATACTCCGCACATAGTTCTAGGGAGTGCAACTGCTTTAATTGGTGATCCTACTGGCAGAATTGAATTAAGGAAAATTTTAGAAGAAAAAGAAATTGTAAAAAACACCAAAACAATTAAAAAACAAATCAAACAGTTTTTAGGTGATGTAATTATTCATGAAAACAAAGTTTGA", "aa": "MLINIDNILVKMLNNILQFLKERELYSQANFETELDNHLKEKKNNFYVGFDPTANSLHIGNYVLIHIAKLLKDMGHTPHIVLGSATALIGDPTGRIELRKILEEKEIVKNTKTIKKQIKQFLGDVIIHENKV", "aa_hexdigest": "e103fb34ae6eaf0f11076bda4d427ce9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15157.552899999984, "isoelectric_point": 8.780739021301269 }, "id": "KKNAAMEMLJ_999", "locus": "GCA000027325_00999" }, { "type": "cds", "sequence": "contig_1", "start": 558239, "stop": 558463, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTAAACTATATGATCTTACAAGCATATGACTTTTATTATCTACATAAAAACCATAATGTCACTTTACAAATAGGTGGAAGTGATCAGTGGGCTAATATTTTGGCTGGTGCTAACTTAATTAAAAGAAAAAATAATGCTAGTGTTTTTGGATTAACTGCTAATTTATTAGTTAAAGCTAACGGAGAAAAAATGGGTAAAACTAGTAGCGGAGCATTATGA", "aa": "MELNYMILQAYDFYYLHKNHNVTLQIGGSDQWANILAGANLIKRKNNASVFGLTANLLVKANGEKMGKTSSGAL", "aa_hexdigest": "37383a3b60e18ebc69a1cf554932a275", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8145.2681, "isoelectric_point": 9.398539543151855 }, "id": "KKNAAMEMLJ_1000", "locus": "GCA000027325_01000" }, { "type": "cds", "sequence": "contig_1", "start": 558548, "stop": 558880, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01479.30", "PFAM:PF22421.1" ], "nt": "ATGCTAACAATGCTTGATAAAAAAGTAATAGATGAATTGTGTAATTTAAAAGGCCCAAAAATTAAACAAACCAAGCAAATGCTAGCCTTTTTAATTACTGAATTAATCCATGGCACTAAAAAAGCAAAAGAAGCACAACAACGATCTGAACTAATATTTAGTAATCAACCAGATCTTGATATTAAGTTAGTAAAAACAAGCACTAATCTAATTGATTATTTAGTTGAAACTAAATTTATTAAAAGTAAATCAGAAGCAAGAAGATTAATTAGTCAAAAAGGTTTGACAATTAACAATAAACACGTTTTAGACTTAAACCAAATAATTGAATGA", "aa": "MLTMLDKKVIDELCNLKGPKIKQTKQMLAFLITELIHGTKKAKEAQQRSELIFSNQPDLDIKLVKTSTNLIDYLVETKFIKSKSEARRLISQKGLTINNKHVLDLNQIIE", "aa_hexdigest": "d061672e48a5804e7774690d6964603d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01479.30", "name": "S4", "length": 39, "aa_cov": 0.35454545454545455, "hmm_cov": 1.0, "evalue": 1.3293303977134068e-09, "score": 32.76255798339844, "start": 71, "stop": 109 }, { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 57, "aa_cov": 0.5181818181818182, "hmm_cov": 0.9649122807017544, "evalue": 5.819874102563797e-11, "score": 37.292030334472656, "start": 53, "stop": 109 } ], "seq_stats": { "molecular_weight": 12634.803399999983, "isoelectric_point": 9.519611167907716 }, "id": "KKNAAMEMLJ_1001", "locus": "GCA000027325_01001" }, { "type": "cds", "sequence": "contig_1", "start": 558939, "stop": 559655, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG456", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47694", "UniRef:UniRef90_P47694" ], "nt": "ATGGGTAATTTCAAGGCAATAGAATCATTTGAATTTTTTGAACAAGGTCCTATAAGCAGCGATATATTACCAAGTTTTTATCCAACTGCTCTTTACAATTTTGAACCACTTCCAAGACAATTTAAAGTTACTTATAAAGATGGTAGTGAATTTGTTTTTGGTCATATTTATGCAATTAAAAGAAGCAGTAATAAAACAGAAAAAGTAGCTTGCTTGATTGCTATTACACCAGCAATTAATAGTGAAAATCATTTCTTTTTAACTGATGCTAACTATTCTTTAAATAAGAATGTGGCTCAGTTTGAAGCACTTACTGAAAACAAAAAGCAAGAAAACATCTCACTTTTTGTTGAAAAAGATTCAAATTTTTCTTTTCAGAATTTAGATACAGAGGTATTAAATAAAGTGCTTTTTAATCCTTTGAATGTATATGCCAAATTTAATGTCTATAATGACACAGTTCATACTTACTTGTTTATGAGTGTGCCTACAACCTTTATGGATACTAAACTAAAAGTGAATGAAGCATTTGATGATCTAGTTACAAACATTAAACTCCAAGCAAGCTATGATTTCAAAACATTAAATTCAATGCAGAAAGTTGTTGATTTACTTCATAACAAATTAATCAAAAAACCAGAAAGTAAAAGCTCTAGTCAAAAAAGTGTTGAAACTGAAATAGAAAAAGAAGTTAAAGATAAATTAGCTAAGAATTAA", "aa": "MGNFKAIESFEFFEQGPISSDILPSFYPTALYNFEPLPRQFKVTYKDGSEFVFGHIYAIKRSSNKTEKVACLIAITPAINSENHFFLTDANYSLNKNVAQFEALTENKKQENISLFVEKDSNFSFQNLDTEVLNKVLFNPLNVYAKFNVYNDTVHTYLFMSVPTTFMDTKLKVNEAFDDLVTNIKLQASYDFKTLNSMQKVVDLLHNKLIKKPESKSSSQKSVETEIEKEVKDKLAKN", "aa_hexdigest": "aae99eb35654d2e235fba60e0322e4ea", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 558939, "stop": 559943, "observations": { "mutation": [ 559667 ], "stop": [ 559667 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47694", "query_cov": 1.0, "subject_cov": 0.7125748502994012, "identity": 1.0, "score": 459.0, "evalue": 6.86e-162, "gene_start": 1, "gene_end": 238, "reference_start": 97, "reference_end": 334, "reference_sequence": "MSDLTKFNKFFLTPNKLNAFLRVIGLCGLFSVIAISFGIYSYTRNEIPNIASLFLIVLGSVVLFLAFVIHFAALFKRNKLLKKVNKENRTNLWQKEMGNFKAIESFEFFEQGPISSDILPSFYPTALYNFEPLPRQFKVTYKDGSEFVFGHIYAIKRSSNKTEKVACLIAITPAINSENHFFLTDANYSLNKNVAQFEALTENKKQENISLFVEKDSNFSFQNLDTEVLNKVLFNPLNVYAKFNVYNDTVHTYLFMSVPTTFMDTKLKVNEAFDDLVTNIKLQASYDFKTLNSMQKVVDLLHNKLIKKPESKSSSQKSVETEIEKEVKDKLAKN" }, "paralog": false, "identity": 0.9970059880239521, "score": 654.0, "evalue": 4.15e-241, "description": "Internal stop codon at 559667. Nonsense mutation around 559667.", "psc": { "uniref90_id": "UniRef90_P47694", "product": "Uncharacterized protein MG456", "uniref50_id": "UniRef50_P47694", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47694", "UniRef:UniRef50_P47694", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47694", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47694" ], "product": "Uncharacterized protein MG456" } }, "id": "KKNAAMEMLJ_1002", "locus": "GCA000027325_01002" }, { "type": "cds", "sequence": "contig_1", "start": 559665, "stop": 559943, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATCTAACTAAATTTAACAAGTTCTTTTTGACACCAAATAAACTCAATGCTTTTCTAAGAGTAATTGGACTTTGTGGTCTTTTTAGTGTTATTGCTATCTCATTTGGTATTTATAGTTATACACGTAATGAAATACCAAATATAGCATCACTTTTTTTAATTGTTTTAGGTAGTGTTGTTTTGTTTTTAGCATTTGTTATCCATTTTGCTGCTCTTTTTAAAAGAAACAAGCTATTAAAAAAAGTTAACAAAGAAAACAGAACTAATTTATGA", "aa": "MSDLTKFNKFFLTPNKLNAFLRVIGLCGLFSVIAISFGIYSYTRNEIPNIASLFLIVLGSVVLFLAFVIHFAALFKRNKLLKKVNKENRTNL", "aa_hexdigest": "85b08d3165963612172bb14e8b2f55bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10457.435899999984, "isoelectric_point": 10.358344841003415 }, "id": "KKNAAMEMLJ_1003", "locus": "GCA000027325_01003" }, { "type": "cds", "sequence": "contig_1", "start": 560291, "stop": 560512, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACAAACAGTTAGTGTTGTTGATTTTATTGATAACTTCTTGGTTAACTTACTATCATTTAAGCAAGAATTAAATGCAATAGAAGTTGGTTATGGTAATGAAGCTTGGAATTGTTTTAAAGTACTAAATGATCAAAGTAAAACAATTACTGATTTTTTTACAGTAATTAAAAATAATCCCCAATTGTTTGACAGTGAATATCACTACATTTTGATTTGA", "aa": "MKQTVSVVDFIDNFLVNLLSFKQELNAIEVGYGNEAWNCFKVLNDQSKTITDFFTVIKNNPQLFDSEYHYILI", "aa_hexdigest": "d49fdaf039ab9cdc6a2134aa7bc293af", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8524.595899999998, "isoelectric_point": 4.5550428390502935 }, "id": "KKNAAMEMLJ_1004", "locus": "GCA000027325_01004" }, { "type": "cds", "sequence": "contig_1", "start": 560496, "stop": 561923, "strand": "-", "frame": 1, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6LLW0" ], "nt": "GTGAAATTTACCAATATTGCTGGACTTCAAGAGGAAAAGCATGAGTTGCTTGAGATAGTTGATTATTTAAAAAATCCATTGAAATATGCCCAGATGGGAGCAAGATCCCCACGTGGGGTAATTTTATACGGTCCACCTGGGACAGGTAAAACATTATTAGCTAAAGCAGTAGCTGGTGAAGCTGGTGTTCCTTTCTTTCAATCAACGGGTTCTGGATTTGAAGATATGCTTGTTGGTGTTGGTGCTAAACGAGTTAGAGATCTTTTCAATAAAGCTAAAAAGGCTGCTCCTTGTATTATTTTTATTGATGAAATTGATTCAGTTGGTTCTAAACGGGGTAGAGTTGAACTCTCTTCTTATTCTGTTGTTGAGCAAACCTTAAACCAATTGTTAGCTGAAATGGATGGATTTACAAGCAGAACAGGTGTTGTTGTAATGGCAGCTACAAATAGGTTAGATGTATTAGATGATGCATTATTAAGACCTGGAAGATTTGATAGACATATTCAAATCAATCTCCCTGATATTAAAGAAAGGGAAGGGATTTTAAAAGTTCATGCTGAAAATAAAAATCTCTCTTCTAAGATAAGTCTTTTAGATGTTGCTAAGAGAACTCCTGGGTTTTCAGGTGCTCAATTAGAAAATGTTATCAATGAAGCTACATTGTTAGCAGTTAGAGACAACCGTACCACAATTAACATTAATGACATTGATGAAGCAATTGATAGAGTAATAGCTGGTCCTGCTAAAAAGTCACGTGTAATTAGTGATGAAGATAGAAAACTAGTTGCTTATCATGAGGCTGGTCATGCCTTGGTTGGTTTACATGTCCACAGTAATGATGAAGTACAAAAGATTACCATTATTCCTCGTGGTCAAGCAGGGGGTTACACACTTTCAACACCTAAGAGTGGTGATCTTAACCTAAAAAGAAAATCTGATTTACTTGCAATGATAGCAACTGCTATGGGCGGTAGAGCTGCTGAAGAGGAAATCTATGGTAATTTAGAAATTACTACTGGCGCTTCTAGCGATTTTTATAAAGCAACTAATATTGCAAGAGCAATGGTAACCCAGCTTGGGATGTCTAAATTAGGTCAAGTGCAATATGTACCAAGTCAAGGGACACTCCCTTCTAATGTAAAACTTTATTCAGAACAAACTGCTAAAGATATTGACAATGAGATTAATTTCATTATTGAAGAACAGTATAAGAAAGCAAAAACAATCATTAAGAGTAACCGTAAGGAACTAGAATTGCTTGTAGAAGCACTTTTAATTGCTGAAACTATTTTGAAAAGTGATATTGACTTCATCCATAAAAACACTAAACTACCACCAGAAATCTTATTGCAAAAGCAAGAACAACAAGCAAAGCAAAAACTAAATAAATCTGAAGTAAAACCAGAAAGTGAAACAAACAGTTAG", "aa": "MKFTNIAGLQEEKHELLEIVDYLKNPLKYAQMGARSPRGVILYGPPGTGKTLLAKAVAGEAGVPFFQSTGSGFEDMLVGVGAKRVRDLFNKAKKAAPCIIFIDEIDSVGSKRGRVELSSYSVVEQTLNQLLAEMDGFTSRTGVVVMAATNRLDVLDDALLRPGRFDRHIQINLPDIKEREGILKVHAENKNLSSKISLLDVAKRTPGFSGAQLENVINEATLLAVRDNRTTININDIDEAIDRVIAGPAKKSRVISDEDRKLVAYHEAGHALVGLHVHSNDEVQKITIIPRGQAGGYTLSTPKSGDLNLKRKSDLLAMIATAMGGRAAEEEIYGNLEITTGASSDFYKATNIARAMVTQLGMSKLGQVQYVPSQGTLPSNVKLYSEQTAKDIDNEINFIIEEQYKKAKTIIKSNRKELELLVEALLIAETILKSDIDFIHKNTKLPPEILLQKQEQQAKQKLNKSEVKPESETNS", "aa_hexdigest": "a4de579dd6ef6f0a905f228af8bba544", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A929X996", "query_cov": 0.9852631578947368, "subject_cov": 0.8283185840707965, "identity": 0.551, "score": 470.0, "evalue": 9.03e-159, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A7V6LLW0", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7V6LLW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6LLW0" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "KKNAAMEMLJ_1005", "locus": "GCA000027325_01005" }, { "type": "cds", "sequence": "contig_1", "start": 562014, "stop": 562172, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAATGGTGGTTCTAGTAATAACACAAAGAGTATTGCAGGTTTTGAAACCCTTGGTACTTTCATTGCTCCTGATACTAGAGCTAGAGATGTATTAAATGGTTTGTTTGGCTTGCTACCAATTATTATCTTTGTAGTTTTCTTTTTACTCTTTTGA", "aa": "MMNGGSSNNTKSIAGFETLGTFIAPDTRARDVLNGLFGLLPIIIFVVFFLLF", "aa_hexdigest": "cd04ed211c784284b2dcf23ac4be6cc0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5637.570399999995, "isoelectric_point": 5.87689037322998 }, "id": "KKNAAMEMLJ_1006", "locus": "GCA000027325_01006" }, { "type": "cds", "sequence": "contig_1", "start": 562605, "stop": 562763, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTGGTTCAAGGCTTTTATTGCACTTTTAAGTAAACATAAAAAGATAATTTTCGTCACTTTATTAACACTTATTAGTTTCATTACCATTGGCAGTATAATTACAGTTAATGTAGTTCAAACCAAAAAAACCCAAATCGGTCAAAAACTAACATAG", "aa": "MRWFKAFIALLSKHKKIIFVTLLTLISFITIGSIITVNVVQTKKTQIGQKLT", "aa_hexdigest": "4d3a29634f9a271594a38a2b92c049f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5932.243300000001, "isoelectric_point": 11.471713829040528 }, "id": "KKNAAMEMLJ_1007", "locus": "GCA000027325_01007" }, { "type": "cds", "sequence": "contig_1", "start": 562782, "stop": 563309, "strand": "-", "frame": 1, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0634", "COG:F", "EC:2.4.2.8", "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657", "KEGG:K00760", "RefSeq:WP_009885573.1", "SO:0001217", "UniParc:UPI000012CB64", "UniRef:UniRef100_P47696", "UniRef:UniRef50_P47696", "UniRef:UniRef90_P47696" ], "nt": "ATGGGTATTAAATCTATTGTTATTAATGAACAACAGATAGAAGAAGGCTGTCAAAAAGCAGTTAATTGGTGCAATGCTAAATTTAATAATAAAAAGGTAATTGTTCTTGGCATTCTAAAAGGTTGCATCCCTTTCCTTGGCAAAGTGATAAGTAAATTTAGTTTTGACCTCCAACTAGATTTTATGGCAGTTGCTTCTTATCATGGTTCACATGTACAAAAACAACCACCTAAGATTGTGCTTGATATGTCCCATGACCCTAAAGATAAAGACATCCTTTTAATAGAAGATATTGTTGATAGTGGTAGATCTATTAAATTAGTTATTGATCTTCTAAAAACAAGGCATGCTAAATCAATAACTTTAATTAGCTTAATTGAAAAGATTAAACCCAAAGCCTTTGATATTAATATTGATTTTTCTTGTTTTAAAGTAAAAGATAATTTTTTGGTTGGCTTTGGTCTTGACTATGATGGTTTTTATCGTAACCTACCTTATGTTGGTGTGTTTGAACCAGACAATCCCTAA", "aa": "MGIKSIVINEQQIEEGCQKAVNWCNAKFNNKKVIVLGILKGCIPFLGKVISKFSFDLQLDFMAVASYHGSHVQKQPPKIVLDMSHDPKDKDILLIEDIVDSGRSIKLVIDLLKTRHAKSITLISLIEKIKPKAFDINIDFSCFKVKDNFLVGFGLDYDGFYRNLPYVGVFEPDNP", "aa_hexdigest": "4242e5c1e66cb0cecb4953ec06aac97f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012CB64", "ncbi_nrp_id": "WP_009885573.1", "uniref100_id": "UniRef100_P47696", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012CB64", "RefSeq:WP_009885573.1", "UniRef:UniRef100_P47696" ] }, "ips": { "uniref100_id": "UniRef100_P47696", "uniref90_id": "UniRef90_P47696" }, "psc": { "uniref90_id": "UniRef90_P47696", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_P47696", "cog_id": "COG0634", "cog_category": "F", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657" ] }, "pscc": { "uniref50_id": "UniRef50_P47696", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47696" ], "product": "Hypoxanthine-guanine phosphoribosyltransferase" }, "genes": [ "hpt" ], "id": "KKNAAMEMLJ_1008", "locus": "GCA000027325_01008" }, { "type": "cds", "sequence": "contig_1", "start": 563512, "stop": 563820, "strand": "-", "frame": 3, "gene": "ispF", "product": "putative protein MG459", "db_xrefs": [ "COG:COG0245", "COG:I", "GO:0008685", "GO:0016114", "SO:0001217", "UniRef:UniRef50_Q49436", "UniRef:UniRef90_Q49436" ], "nt": "ATGCAGTTAAGAGTTGGCCTAGGAAGCAAAAAATACCACATAAAATTAAGGAAAGAAAGTAAAAATAAATTTTGGTTAGGCGGTGTTGATTTTGAGAATTCTAAATATATTTATGATCTTGAAAACAGTGATGAAGTTAGTGATGTTATCCAATTAGCAGTTGCTGATGCTTTATTTGGTGCAACTGCATTAGGTGATGGTCATATTGTTTTCAATAAAGGAAAATCAACAGTCCAATTTAAAGGTACTGCAAAAAAAGAAGCACCAAGAGTCTTAGCAAGAACTTACAATTTCATCAGAAAAAACTGA", "aa": "MQLRVGLGSKKYHIKLRKESKNKFWLGGVDFENSKYIYDLENSDEVSDVIQLAVADALFGATALGDGHIVFNKGKSTVQFKGTAKKEAPRVLARTYNFIRKN", "aa_hexdigest": "3b1ee3dd0f6b51c00f9bed5437326814", "start_type": "ATG", "rbs_motif": null, "genes": [ "ispF" ], "truncated": "5-prime", "pseudogene": { "start": 563311, "stop": 563820, "observations": { "mutation": [ 563514 ], "stop": [ 563514 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49436", "query_cov": 1.0, "subject_cov": 0.6035502958579881, "identity": 1.0, "score": 202.0, "evalue": 3.4e-65, "gene_start": 1, "gene_end": 102, "reference_start": 1, "reference_end": 102, "reference_sequence": "MQLRVGLGSKKYHIKLRKESKNKFWLGGVDFENSKYIYDLENSDEVSDVIQLAVADALFGATALGDGHIVFNKGKSTVQFKGTAKKEAPRVLARTYNFIRKNWIINNIDISLEIPQEQKVDDYKHAIFAFICSALRISELTINLKVRKPLDSNEINCLAVVLVERQKTK" }, "paralog": false, "identity": 0.9940828402366864, "score": 330.0, "evalue": 4.19e-118, "description": "Internal stop codon at 563514. Nonsense mutation around 563514.", "psc": { "uniref90_id": "UniRef90_Q49436", "gene": "IspF", "product": "Uncharacterized protein MG459", "uniref50_id": "UniRef50_Q49436", "cog_id": "COG0245", "cog_category": "I", "go_ids": [ "GO:0008685", "GO:0016114" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49436", "UniRef:UniRef50_Q49436", "COG:COG0245", "COG:I", "GO:0008685", "GO:0016114" ] }, "pscc": { "uniref50_id": "UniRef50_Q49436", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49436" ], "product": "Uncharacterized protein MG459" } }, "id": "KKNAAMEMLJ_1009", "locus": "GCA000027325_01009" }, { "type": "cds", "sequence": "contig_1", "start": 563993, "stop": 564931, "strand": "+", "frame": 2, "gene": "ldh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "EC:1.1.1.27", "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006090", "GO:0006096", "KEGG:K00016", "RefSeq:WP_009885571.1", "SO:0001217", "UniParc:UPI000012E33D", "UniRef:UniRef100_P47698", "UniRef:UniRef50_P78007", "UniRef:UniRef90_P47698" ], "nt": "ATGAAAGGACCTAAAATTGCAATAGTAGGATCAGGTGCTGTTGGTACTAGTTTTCTATATGCTGCAATGACTCGTGCACTTGGTAGTGAATACATGATTATAGATATCAATGAAAAAGCTAAAGTTGGTAATGTATTTGATCTTCAAGATGCTTCCTCATCATGCCCAAATTTTGGCAAAGTAGTTGCTGGAGAATACAGTCAATTAAAAGATTATGATTTTATTTTCATTAGTGCAGGAAGACCTCAAAAACAAGGTGGGGAAACTAGGTTGCAGCTACTTGAAGGCAATGTAGAAATTATGAAAAGCATTGCCAAAGAAATCAAAAAATCTGGTTTTAATGGAGTTACATTAATCGCTTCAAACCCAGTTGATATCATGTCATATACATATCTTAAAGTTACTGGATTTGAACCTAATAAAGTAATTGGCTCTGGTACTTTACTTGATAGTGCAAGATTAAGATATGCAATTGCAACTAAGTACCAAATGAGTTCAAAGGATGTACAAGCATATGTTATTGGTGAACATGGTGATAGTTCAGTAAGTATTATTTCATCAGCTAAAATTGCAGGACTATCACTAAAACATTTTTCTAAAGCTAGTGATATTGAAAAAGAATTTGGTGAAATTGACCAATTTATCAGACGCAGAGCTTATGAAATTATTGAACGCAAAGGTGCTACTTTCTATGGAATTGGTGAAGCTAGTGCTGATGTAGCTGAACAAATTTTGAAAGATACTAAAGAAGTTAGAGTAGTGGCTCCTTTACTTACTGGTCAGTATGGAGCGAAGGATATGATGTTTGGAACTCCTTGTGTACTTTCAAGAAAAGGTATTGAAAAGATCTTGGAAATAGAACTTTCAAATACTGAAAAAGTTGCGCTTGAAAATTCAATTAAAGTTTTGAAAGACAACATTAAACTAGCAAAGCTTTAG", "aa": "MKGPKIAIVGSGAVGTSFLYAAMTRALGSEYMIIDINEKAKVGNVFDLQDASSSCPNFGKVVAGEYSQLKDYDFIFISAGRPQKQGGETRLQLLEGNVEIMKSIAKEIKKSGFNGVTLIASNPVDIMSYTYLKVTGFEPNKVIGSGTLLDSARLRYAIATKYQMSSKDVQAYVIGEHGDSSVSIISSAKIAGLSLKHFSKASDIEKEFGEIDQFIRRRAYEIIERKGATFYGIGEASADVAEQILKDTKEVRVVAPLLTGQYGAKDMMFGTPCVLSRKGIEKILEIELSNTEKVALENSIKVLKDNIKLAKL", "aa_hexdigest": "99940a250ddf1c25b4687a3ef06d9c6e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E33D", "ncbi_nrp_id": "WP_009885571.1", "uniref100_id": "UniRef100_P47698", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E33D", "RefSeq:WP_009885571.1", "UniRef:UniRef100_P47698" ] }, "ips": { "uniref100_id": "UniRef100_P47698", "uniref90_id": "UniRef90_P47698" }, "psc": { "uniref90_id": "UniRef90_P47698", "gene": "ldh", "product": "L-lactate dehydrogenase", "ec_ids": [ "1.1.1.27" ], "uniref50_id": "UniRef50_P78007", "cog_id": "COG0039", "cog_category": "C", "kegg_orthology_id": "K00016", "go_ids": [ "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006090", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P78007", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78007" ], "product": "L-lactate dehydrogenase" }, "genes": [ "ldh" ], "id": "KKNAAMEMLJ_1010", "locus": "GCA000027325_01010" }, { "type": "cds", "sequence": "contig_1", "start": 565075, "stop": 565344, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAAAAAGTTTATTAACTTGTTTTGTGAAGTTAGATGATTATGTTTTTCACTCATTTTTAGTTAATACATTTGAAACAACTAAAGATGCAATATTAAAAACCTTACTAGATAGTTATCTTAACACTCTGAAATTTCAAGTAAAGTTTTATGAAAGTTGTGAAAAACGTGATCTTGATTTTGAATTAAAAGTAAAAGAATATCAAACTCCTAGTTATTTCATTACTAAATTCAATAACCAATTTAAGGGTTTTTATGAAGGATGA", "aa": "MFKKSLLTCFVKLDDYVFHSFLVNTFETTKDAILKTLLDSYLNTLKFQVKFYESCEKRDLDFELKVKEYQTPSYFITKFNNQFKGFYEG", "aa_hexdigest": "bc75fa5df9c9026d37728f81e0de5c91", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10731.238099999993, "isoelectric_point": 7.705798530578614 }, "id": "KKNAAMEMLJ_1011", "locus": "GCA000027325_01011" }, { "type": "cds", "sequence": "contig_1", "start": 565447, "stop": 566205, "strand": "-", "frame": 3, "gene": "ydhJ", "product": "putative protein MG461", "db_xrefs": [ "COG:COG1078", "COG:R", "GO:0006203", "GO:0008832", "SO:0001217", "UniRef:UniRef50_P47699", "UniRef:UniRef90_P47699" ], "nt": "ATGCAACAAACCTTTTTTAAAGATCCTATTTTAGGAGAAATTATTTTTGATGAAAACACAAAGTGGATGTATGAATTGGTCAATACAAAAGCTTTTCAAAGGTTAAGAAACATCAAACAATTAGGAATTAACTTCCATTTTTATCCTAGTGGAGTTCACACTAGGTACGCCCATTCTTTAGGAGTTTATGAACTGATAAGAAGGATCTTAAATAGTTCTGCATTTTTAAATATTGATCAAATTAAAAAACAAACTGTTTTAGTAGCTGGTTTGTTGCATGATCTTGGTCATGGACCTCATTCTCATGCTTTTGAAATCTATTTTGCTAAAAATCCTGATTTTAAAAAACAACTTTTTATTCATGAAAAGGTTACTTCAATGTTAGTTAATAGTGAACCTATTGTCTCTATCTTAAAAGCTAATAAGATAGATCCAAATCTGATTGGCGCTTTAATTGATGAAAATCAAAATATTCAACCTATTAACTGGTGGATGAGACAGCTAATCTCTTCAGATCTTGACACAGACAGAATGGATTATTTATTACGTGATGCTTATTTTACAGGTACCAGTCACTCATTAGTTGATTATCAATCAATTATCAATGGAATGGAATGTGTAGATAATCAGGGCACTTATGAAATTGTTTTTCAAGAAAAATGCTTGCCTTTCATTGAAAACTTCTTGATAACAAGACACCACATGTATCAATCAATCTACAGTGATGGTAGAAGTATTGCTACAGAACTTAACTTATGA", "aa": "MQQTFFKDPILGEIIFDENTKWMYELVNTKAFQRLRNIKQLGINFHFYPSGVHTRYAHSLGVYELIRRILNSSAFLNIDQIKKQTVLVAGLLHDLGHGPHSHAFEIYFAKNPDFKKQLFIHEKVTSMLVNSEPIVSILKANKIDPNLIGALIDENQNIQPINWWMRQLISSDLDTDRMDYLLRDAYFTGTSHSLVDYQSIINGMECVDNQGTYEIVFQEKCLPFIENFLITRHHMYQSIYSDGRSIATELNL", "aa_hexdigest": "41720e1b1bc383fcce95409d307405d8", "start_type": "ATG", "rbs_motif": null, "genes": [ "ydhJ" ], "truncated": "5-prime", "pseudogene": { "start": 565144, "stop": 566205, "observations": { "mutation": [ 565449 ], "stop": [ 565449 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47699", "query_cov": 1.0, "subject_cov": 0.5929411764705882, "identity": 1.0, "score": 515.0, "evalue": 2.59e-182, "gene_start": 1, "gene_end": 252, "reference_start": 1, "reference_end": 252, "reference_sequence": "MQQTFFKDPILGEIIFDENTKWMYELVNTKAFQRLRNIKQLGINFHFYPSGVHTRYAHSLGVYELIRRILNSSAFLNIDQIKKQTVLVAGLLHDLGHGPHSHAFEIYFAKNPDFKKQLFIHEKVTSMLVNSEPIVSILKANKIDPNLIGALIDENQNIQPINWWMRQLISSDLDTDRMDYLLRDAYFTGTSHSLVDYQSIINGMECVDNQGTYEIVFQEKCLPFIENFLITRHHMYQSIYSDGRSIATELNLWFVFQRIKALIEEDNFNFHNFKNVESVIKPLLKNQLFKKSLLTCFVKLDDYVFHSFLVNTFETTKDAILKTLLDSYLNTLKFQVKFYESCEKRDLDFELKVKEYQTPSYFITKFNNQFKGFYEGWNKHKNELKIKTSQNKIKNLSEISMLVKRSNELFFENSFYRWANVFYQN" }, "paralog": false, "identity": 0.9971671388101983, "score": 712.0, "evalue": 2.17e-262, "description": "Internal stop codon at 565449. Nonsense mutation around 565449.", "psc": { "uniref90_id": "UniRef90_P47699", "gene": "YdhJ", "product": "Uncharacterized protein MG461", "uniref50_id": "UniRef50_P47699", "cog_id": "COG1078", "cog_category": "R", "go_ids": [ "GO:0008832", "GO:0006203" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47699", "UniRef:UniRef50_P47699", "COG:COG1078", "COG:R", "GO:0008832", "GO:0006203" ] }, "pscc": { "uniref50_id": "UniRef50_P47699", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47699" ], "product": "Uncharacterized protein MG461" } }, "id": "KKNAAMEMLJ_1012", "locus": "GCA000027325_01012" }, { "type": "cds", "sequence": "contig_1", "start": 566186, "stop": 566344, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "GTGTTTAACTTAAAAGGTAAACAACTTTTTATGCCAATAAGGTTAATTTTTACAAATAAGGAGCATGGACCTGATTTAGCACATATTATTGAAATTTTTGATAAAGAAAGTGCAATAAACTTAATTAAGCAATTTATTAATGCAACAAACCTTTTTTAA", "aa": "MFNLKGKQLFMPIRLIFTNKEHGPDLAHIIEIFDKESAINLIKQFINATNLF", "aa_hexdigest": "df98cf08eec54401ee998c3ec3e4ea52", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 43, "aa_cov": 0.8269230769230769, "hmm_cov": 1.0, "evalue": 2.6299374816923956e-08, "score": 29.451568603515625, "start": 3, "stop": 45 } ], "seq_stats": { "molecular_weight": 6074.122299999998, "isoelectric_point": 8.181634330749514 }, "id": "KKNAAMEMLJ_1013", "locus": "GCA000027325_01013" }, { "type": "cds", "sequence": "contig_1", "start": 566387, "stop": 566608, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTTAAGGTTGATTATTTAAAAGATAAAAACAGGGAAATAAGTTTACTTTTTAAAAATCAAATAACCCATGGTGTTCAAATAAACGAATTGATAAGAGAATCTTTTGCCACTAAAATAGGTGTTGAAAACTTAGCTAAGAAAAGTCATATTTTGTTTAAAAACATCAAACTTTTTTTAGAACAGCTTGCCAAATCTTTACAAGGGTTGGAAGAATGA", "aa": "MDVKVDYLKDKNREISLLFKNQITHGVQINELIRESFATKIGVENLAKKSHILFKNIKLFLEQLAKSLQGLEE", "aa_hexdigest": "4d338d3cba2ef8fdbfe49036ece3fb04", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8469.808099999997, "isoelectric_point": 9.004702186584474 }, "id": "KKNAAMEMLJ_1014", "locus": "GCA000027325_01014" }, { "type": "cds", "sequence": "contig_1", "start": 566783, "stop": 567025, "strand": "-", "frame": 2, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "nt": "ATGGAAATTACTGATGTTTTAAGGGGAGCTGAGCACATCTCTAACACTGCATACCAACTTGCTATATATCAAGCATTAGGTTTTAAAAGAATTCCCCGCTTTGGTCATCTTTCAGTTATTGTTGATGAAAGTGGCAAAAAACTTTCTAAACGTGATGAGAAAACTACTCAGTTTATTGAGCAGTTTAAACAACAAGGCTATCTACCTGAAGCATTATTAAATTTCTTAGCACTCTTAGGTTGA", "aa": "MEITDVLRGAEHISNTAYQLAIYQALGFKRIPRFGHLSVIVDESGKKLSKRDEKTTQFIEQFKQQGYLPEALLNFLALLG", "aa_hexdigest": "648d89fc9c6321a5d78c0a33260bc56c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003594285F", "query_cov": 0.875, "subject_cov": 0.8235294117647058, "identity": 0.6, "score": 85.9, "evalue": 2.12e-20, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI003594285F" }, "pscc": { "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "product": "glutamate--tRNA ligase family protein" }, "genes": [], "id": "KKNAAMEMLJ_1015", "locus": "GCA000027325_01015" }, { "type": "cds", "sequence": "contig_1", "start": 567143, "stop": 567640, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00749.27" ], "nt": "ATGGAAAAAATTAGAACACGTTATGCACCATCCCCAACAGGATATCTGCATGTTGGTGGTACAAGAACAGCAATCTTTAACTTTTTACTAGCCAAGCACTTTAATGGTGAGTTTATTATCAGGATAGAAGATACTGATACTGAAAGAAACATAAAAGAAGGAATTAATTCACAATTTGATAACTTGCGTTGGCTTGGAGTCATTGCAGATGAATCGGTTTATAACCCTGGCAATTATGGTCCATATCTGCAATCACAAAAACTAGCAGTTTATAAAAAACTAGCATTTGATTTAATTGAAAAAAATCTGGCATATCGTTGCTTCTGTAGCAAAGAAAAATTAGAGTCAGATAGAAAACAAGCCATTAATAACCACAAAACCCCTAAATACTTAGGTCATTGTCGTAATTTACATTCCAAGAAAATTACTAATCACTTAGAAAAAAATGATCCTTTTACTATCCGCTTAAAAATAAACAATGAAGCTGAATATAGTTGA", "aa": "MEKIRTRYAPSPTGYLHVGGTRTAIFNFLLAKHFNGEFIIRIEDTDTERNIKEGINSQFDNLRWLGVIADESVYNPGNYGPYLQSQKLAVYKKLAFDLIEKNLAYRCFCSKEKLESDRKQAINNHKTPKYLGHCRNLHSKKITNHLEKNDPFTIRLKINNEAEYS", "aa_hexdigest": "a1bd8d8224c3b4a54fc6b7dc7310f2bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00749.27", "name": "tRNA-synt_1c", "length": 163, "aa_cov": 0.9757575757575757, "hmm_cov": 0.950920245398773, "evalue": 1.2456162572420699e-54, "score": 180.3483428955078, "start": 3, "stop": 163 } ], "seq_stats": { "molecular_weight": 19212.628600000015, "isoelectric_point": 9.282238578796388 }, "id": "KKNAAMEMLJ_1016", "locus": "GCA000027325_01016" }, { "type": "cds", "sequence": "contig_1", "start": 567627, "stop": 568406, "strand": "-", "frame": 1, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0031167", "GO:0052908", "RefSeq:WP_010869490.1", "SO:0001217", "UniParc:UPI000012E0AD", "UniRef:UniRef100_P47701", "UniRef:UniRef50_P47701", "UniRef:UniRef90_P47701" ], "nt": "GTGAATAGTTTTTTTCCTTCACGTAAATTAGGTCAAAATTTTACGGTTAATTTAAGCGTCATTAAAAGAATTTTTGCTTTTGTTAAAAATTTAAATCCACAAGCAATTGTTGAAATAGGTGTTGGTAAAGGAGCGTTAACAAATTATTTGTTAAAACTCAAAATACCTTACAAGGGGATAGAAATTGATAAACGCTTAATTGAATATCTTCTAGTTGAAAAGATATTAACTGAAGACCAACTAGTTAAAGGCGATATTCTCAAAAAGGACTTTAATAGTTTTTTTGAAAATTTAAGTCCATTATTGTGTGGTAATATCCCATATAGCATTACATCTCCAATAATCAATAAGTTTTTAGAATCAAAGCTTCGAAGCTTTGTTTTAATGACACAAAAAGAGTTTGCTAATCGGCTTCTGGCAAAGGTTAATTCTAGTGATTATAGTGCCTTTGGTGCTTTTTGTCAATACTATTTGACTATTACAACAGTTTTTAAAATTGATAGGCATGCTTTTAAACCTAAACCTAAGGTGGATTCAACCTTAATATTATTAGAAAAAAATAAATCGGTTAGTTATGACTTTAAGTTTGGTCTGTTTTTAAAGCAATGTTTCAATCAAAGAAGGAAGATGTTAATTAATAACTTAAAGCATTTTTTTGCAGTTGATTATTTACTTAACATTATTCAAAAGCAAAATTTAAAGACTAGCATTAGAGCGCAAGAGTTGAGTCCTTGTCAACTCTTTAATCTTTACCAAAACATTTGTAATGGAAAAAATTAG", "aa": "MNSFFPSRKLGQNFTVNLSVIKRIFAFVKNLNPQAIVEIGVGKGALTNYLLKLKIPYKGIEIDKRLIEYLLVEKILTEDQLVKGDILKKDFNSFFENLSPLLCGNIPYSITSPIINKFLESKLRSFVLMTQKEFANRLLAKVNSSDYSAFGAFCQYYLTITTVFKIDRHAFKPKPKVDSTLILLEKNKSVSYDFKFGLFLKQCFNQRRKMLINNLKHFFAVDYLLNIIQKQNLKTSIRAQELSPCQLFNLYQNICNGKN", "aa_hexdigest": "54b49b755d41550070f0724426524017", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E0AD", "ncbi_nrp_id": "WP_010869490.1", "uniref100_id": "UniRef100_P47701", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E0AD", "RefSeq:WP_010869490.1", "UniRef:UniRef100_P47701" ] }, "ips": { "uniref100_id": "UniRef100_P47701", "uniref90_id": "UniRef90_P47701" }, "psc": { "uniref90_id": "UniRef90_P47701", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_P47701", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0031167", "GO:0052908" ] }, "pscc": { "uniref50_id": "UniRef50_P47701", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47701" ], "product": "Ribosomal RNA small subunit methyltransferase A" }, "genes": [ "rsmA" ], "id": "KKNAAMEMLJ_1017", "locus": "GCA000027325_01017" }, { "type": "cds", "sequence": "contig_1", "start": 568399, "stop": 568713, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGTTTTACCAGTCCAATTTCTCTCTCAAAAACTTCCTCAAGTGTGGGCAAGTAAGCGTAATGAGAATGCGAAAGCCCATTCTCAAAAAAGTATTGAACAACTCAACAAAACTAAAAAGATGCAATTAATTTTTTACTTTGTGTTTGCTGCTATTACTGCATTTAGTGCTGCTGGAGTTGGTGTTTATTGGTTTTTAAATGCTTTGTTTACTTTATTACAATCTTATTTAACCCATGTTTTCATTGTCAAAAGAAGGGAAAAAAGAAAGCAAAATTATTCCAAATTAGATTTGATTTTAGAGCGTGAATAG", "aa": "MIVLPVQFLSQKLPQVWASKRNENAKAHSQKSIEQLNKTKKMQLIFYFVFAAITAFSAAGVGVYWFLNALFTLLQSYLTHVFIVKRREKRKQNYSKLDLILERE", "aa_hexdigest": "3b9395dc01ddeff6ab975b8eff9e27a7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12209.249899999986, "isoelectric_point": 10.167453956604003 }, "id": "KKNAAMEMLJ_1018", "locus": "GCA000027325_01018" }, { "type": "cds", "sequence": "contig_1", "start": 568732, "stop": 569160, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCAACCAGAGTTCCACTAGGTAGCGGGGTGGAACTTGGGTTTAACATGATTCTTTCATTAATAGTACTTTTACTATTAGTTCGCTTGATAACTATTGTGATTACCTTAAACTCTACTTTAGCACTTGAAAAGATGAATGAAGTGCAAGGTAAATTAGCAGAAATTAACGCTAAATATAAAGGGGCAATAGATTTACAAAGTAAAAGAAACAGACAGTTAGAGATAATGTCACTTTATAAAAAACACAACATTAAATCATCTGCTGCTTTTGTTCAAGTTTTTGTAACTTTACCTATCTTTTTAATTATTTATAGGATTGTTACTACTTTAAGACCAATTAAAGCAATCATCTTATTTAACTTTTGGGATCTTTCAAAAGTACCTTTAACAGAAATTTTCTCTAATTTCACTACAACTGGTTGA", "aa": "MYATRVPLGSGVELGFNMILSLIVLLLLVRLITIVITLNSTLALEKMNEVQGKLAEINAKYKGAIDLQSKRNRQLEIMSLYKKHNIKSSAAFVQVFVTLPIFLIIYRIVTTLRPIKAIILFNFWDLSKVPLTEIFSNFTTTG", "aa_hexdigest": "4e3e1113c7868ad5bacbfa31eb30f007", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16044.083099999989, "isoelectric_point": 9.992873573303225 }, "id": "KKNAAMEMLJ_1019", "locus": "GCA000027325_01019" }, { "type": "cds", "sequence": "contig_1", "start": 569883, "stop": 570029, "strand": "-", "frame": 1, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_009885567.1", "SO:0001217", "UniParc:UPI0000133FB9", "UniRef:UniRef100_P47704", "UniRef:UniRef50_P0A7P5", "UniRef:UniRef90_P47704" ], "nt": "ATGAAACGAACATACCAACCAAGCAAATTAAAGCGTGCTAAAACCCATGGTTTTATGGCTAGGATGGCAACTGCACAAGGACGTAAAGTTTTAAGGCAAAGACGTTTTAAAAATCGTGCTCAACTCACGGTTTCCAGTGAGCGTTAA", "aa": "MKRTYQPSKLKRAKTHGFMARMATAQGRKVLRQRRFKNRAQLTVSSER", "aa_hexdigest": "b7a51c6f33bc053523970850e910c399", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FB9", "ncbi_nrp_id": "WP_009885567.1", "uniref100_id": "UniRef100_P47704", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FB9", "RefSeq:WP_009885567.1", "UniRef:UniRef100_P47704" ] }, "ips": { "uniref100_id": "UniRef100_P47704", "uniref90_id": "UniRef90_P47704" }, "psc": { "uniref90_id": "UniRef90_P47704", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P0A7P5", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P0A7P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A7P5" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "KKNAAMEMLJ_1020", "locus": "GCA000027325_01020" }, { "type": "cds", "sequence": "contig_1", "start": 570055, "stop": 570450, "strand": "-", "frame": 3, "gene": "lolD", "product": "Putative ABC transporter ATP-binding protein MG467", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75110", "UniRef:UniRef90_P47705" ], "nt": "TTGTTAAGTGCTTATGATAATATTGCCATTTCACAAAACTTAATTCCCAAATATCAAAGACGACTTGATATAGAAGAGTTGGCTGAAAAATTAGATATTAAAGAAATCTTGTATAAATTTCCTTATGAGATGTCAGGTGGGCAAAAACAAAGAGTGGCCATCGCCCGTGCTATTATTAAAGAACCTAAACTATTACTTTGTGATGAACCTACTGGTGCACTTGATAGTAATTCTGCGGAAAATATCATTAATTTATTACAAACAATTAACAAAACATATAAGCAAACCATCCTAATGGTTACCCATGATGTAAGCCTAACAAGGATAGCTAATAGAATCATTAAAATTTCAGATGGTAAAATAGTATCTAACCAATTAGTCAGACCCTTAGTTTAG", "aa": "MLSAYDNIAISQNLIPKYQRRLDIEELAEKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPKLLLCDEPTGALDSNSAENIINLLQTINKTYKQTILMVTHDVSLTRIANRIIKISDGKIVSNQLVRPLV", "aa_hexdigest": "cedc8223e3c5267caaa023ccf721204d", "start_type": "TTG", "rbs_motif": null, "genes": [ "lolD" ], "truncated": "3-prime", "pseudogene": { "start": 570055, "stop": 570750, "observations": { "mutation": [ 570723 ], "stop": [ 570723 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47705", "query_cov": 1.0, "subject_cov": 0.4212218649517685, "identity": 0.992, "score": 249.0, "evalue": 4.28e-81, "gene_start": 1, "gene_end": 131, "reference_start": 181, "reference_end": 311, "reference_sequence": "MKKGNKTVWNECIDILDNVKSPSFSANFDDYFKKSKSKPPKKNKKVLNNIKKAELKLKKKANKKQKANTLYIPPFAQQAKGIVITINKMWKNVHNDDSKQEISILSDVSLQIAYGEIVIILGSSGSGKTTLLNLIGGYDSISLGSCIVANCPLEKCTSEQLLTYRKNNLGYVYQRYNLIELLSAYDNIAISQNLIPKYQRRLDIEELAEKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPKLLLCDEPTGALDSNSAENIINLLQTINKTYKQTILMVTHDVSLTRIANRIIKISDGKIVSNQLVRPLV" }, "paralog": false, "identity": 0.9956709956709957, "score": 444.0, "evalue": 3.67e-160, "description": "Internal stop codon at 570723. Nonsense mutation around 570723.", "psc": { "uniref90_id": "UniRef90_P47705", "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG467", "uniref50_id": "UniRef50_P75110", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47705", "UniRef:UniRef50_P75110", "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75110" ], "product": "Putative ABC transporter ATP-binding protein MG467 homolog" } }, "id": "KKNAAMEMLJ_1021", "locus": "GCA000027325_01021" }, { "type": "cds", "sequence": "contig_1", "start": 571033, "stop": 571386, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATCAAGTAGATGCGTTTCTTTTAGGAACTATTATTCCTTTCATTTTCATTACTTGTGTTGTACTTGGTATCTCAATGTTAGAAGAGATGAAAAGAATCTTTATTTCACTTAAGGCTATTGGCTATAGAGATGTACAAAATTTAATTAGTTTACTTACCTTCTTTATCCCTGCATTTGTTTTAGCATTACTAATTTCTATTGGAGTTTTAGCTGGTGTTTTAATTGGTATCCAAGCGGTTGTTTTCAATGTTGCACAAGTCTTTTTAACTAATGTATTTGAGTTCTTGCCATACATGGTAGGGATAGTATTATTTGGTGTTACTATCTTTGTAATTGGTTCTTACTTTTGA", "aa": "MNQVDAFLLGTIIPFIFITCVVLGISMLEEMKRIFISLKAIGYRDVQNLISLLTFFIPAFVLALLISIGVLAGVLIGIQAVVFNVAQVFLTNVFEFLPYMVGIVLFGVTIFVIGSYF", "aa_hexdigest": "88d4140422110cf46f82bfa01e97becf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12914.61039999999, "isoelectric_point": 4.935693550109861 }, "id": "KKNAAMEMLJ_1022", "locus": "GCA000027325_01022" }, { "type": "cds", "sequence": "contig_1", "start": 571522, "stop": 573243, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTAAACCTGTTAATGTCTTAATTGATCAAGGCAATGAGACCAAGTTTTCAGTTCTTCTTCATCTTTTAAAACATAAAAGTCATACAATGTCACCCTTGACCAAGTTTAGGGTTTCATTAATCGTTTCACGTTTTTCACGTTTATTTACCTATGTAGGATTGAGTTCAGTTGCACTTTTATTAATTGGGATTGCAGGTACTATTCCCCAAAAATTTAGTGCAGCACAAACAAGCACTTCCTTAAATAGAAACTTTAACTATAAGTTGAATTTACAAACACCAACAGAGCAATCTGGATGGTATGCAATTCAACCCTATTCACACTTTGGAGTTACTGATAATAATAATGGTATTAAAACCTTGTATAACGAAAGTGTTCAAGCAAATTCTCAAAATGAGCACCCTTATAAACCAAGTAATTTAAAACTTAAAAACCGCCAGGATCAACCAATAAAAGCAGCAGATGGAACTGAGCTTGAATTGGGCAACTTACTTTTGCCTAGTTATGGTGGTGCTCAACAGTTAAACACTGATGAGAATTTCTTTAGGCATGCCTCTCTATCAAAATGGATTATTGACTTTCCTATTAGGGTTGGCGGTTCTAACATTAACCCTTGGGAAATTGTTGAAAAATCAATTCCTAAACAGATTACTCAGTTACTTTCAGCATCTAGTGATCAGTTTTTAATTTCAGTATTAACTGATGACTTTTTCAATAACTTAAATGCTAATGGGTTTTTAATTAGAAACCCTAGAACTAATCAAATTCAGTTAGATGCTAGTAGGGTACTAACTACAATTGATGTATTTAATCCTGGTGGGGTTAAATTTAATGATAGCTTTCTTAGTTTTATGTTGAAAGTTTATGGGGATTTTGAACTTGCAAAACAAGATTCTAAGTTAAATTTTGGGATTGTTCCTGTTGACCCTGCCATAGAAGAAACCTACACTTATGTTGAAGGCCCATTTGGTTTTCAAGAAGATAATCTTGATGAGAATTCACCATACACTTTAACAGGGATTAACCCTGAATCAAGTTTTGTTAATCTGATAGATGGTAGTGGTAACTCTTTAAGAAATTTAATTAGTTCTGATCAGGAAATGAATGTAATTGTTAATGCTGGTTTTCAATATGCTAACAACATTAATATTGGTGATTATGTTTATATAAAACCGAAAAATACTGCCACAAGATACTCTGAAAAATTTCTAAAAGCACCCTTAAATAATTCAACAGTAGCATTTAAAGTTGTTGGTGTTTCTACTGATGCTTTTGGACAAGAACTTTATATTAACCAACACATTGCTAATAACTTATTAAAACTAAGTGGTAATCAGGGCAGAGGTATTATTAGAGATGTTATTAAAAAGACAAATGGCCAATCTCAAAGTAGTGATGAATATGAAATTGATTATGTAAAACCAAACGGTTATGTTCCTTTTAATGGTGTATTTTCCAAAGAATTAAAACCTTCTTTATTAAATAAGGCATTAGTATTAAACTCGATAATTGGTGTTTGGGGTAATTTCACTGACTTTGGTAATAATTTCCAAAATTTAGTAAGGAATAAACTTGATAAAGTTATTACATCCATACTACCTACTGATCCTGAAATTATTAATAAGTTAGCACAAGAAAAACAGATTATTAATACTACTTCAATGAATTATGAATCGCTTCGTAAAGAACTAGTTAATAAATACAAAACAGAATGA", "aa": "MFSKPVNVLIDQGNETKFSVLLHLLKHKSHTMSPLTKFRVSLIVSRFSRLFTYVGLSSVALLLIGIAGTIPQKFSAAQTSTSLNRNFNYKLNLQTPTEQSGWYAIQPYSHFGVTDNNNGIKTLYNESVQANSQNEHPYKPSNLKLKNRQDQPIKAADGTELELGNLLLPSYGGAQQLNTDENFFRHASLSKWIIDFPIRVGGSNINPWEIVEKSIPKQITQLLSASSDQFLISVLTDDFFNNLNANGFLIRNPRTNQIQLDASRVLTTIDVFNPGGVKFNDSFLSFMLKVYGDFELAKQDSKLNFGIVPVDPAIEETYTYVEGPFGFQEDNLDENSPYTLTGINPESSFVNLIDGSGNSLRNLISSDQEMNVIVNAGFQYANNINIGDYVYIKPKNTATRYSEKFLKAPLNNSTVAFKVVGVSTDAFGQELYINQHIANNLLKLSGNQGRGIIRDVIKKTNGQSQSSDEYEIDYVKPNGYVPFNGVFSKELKPSLLNKALVLNSIIGVWGNFTDFGNNFQNLVRNKLDKVITSILPTDPEIINKLAQEKQIINTTSMNYESLRKELVNKYKTE", "aa_hexdigest": "ad8e3e1e3bec3d295148268c1832a30c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 64159.68070000007, "isoelectric_point": 7.075681495666504 }, "id": "KKNAAMEMLJ_1023", "locus": "GCA000027325_01023" }, { "type": "cds", "sequence": "contig_1", "start": 573259, "stop": 574032, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "TTGCAAACACCAAACAATTTCCCTAACTGGTTAAATGATCTACCTGATCGTTATAAATTTAGTATTAACGGACTAACATTTGCAATTTTAGGTATAGGTGAATCAGTAGAAACTGGTTACCCAGTGTTATCTTTACAATCTCCGTTGCCTAATACCCAAGATGAAGCGTTAATCTTTGTTAATGATCAAGCTTACAGAAGTATTCTGTTTGCTGTACCAGCAGCTAACCAAGAAAATTATTATGCTTTTAAGTCAACAGATTTAAAACAGCACACTGACCAAGACCCAGTGCAGTTTATTGCTAATAGATTAGAGGGATATCTAGATGTTCCTAGAAGTGATTTAGCATTTAATGTTAAAGATATTTCTAAGTTTAATTATCTGACAACAGCAAGAAACTATTTTCCTGATTTAGTGCAAAGCTATTTGGCAATAGTAAGTACTGTTATTGCTATATTTTTGATTATTCTTGCTCTTTATTTAATTATTCTTTTAATTAAGTCATTTATTAAAAAGAATCAAACTGAGTTTTCTATTATCAGAGCAGGGGGATTTTCAACAACTAAATTTATTGTTGGAATGAGTGTGTTTGCTGGTATTGTTGCAATTGTTTCTAGTTTTTTAGGAGTTTTATTTGCCTTTCTTCTAGAAGGACAAGTAAAGGGAATCATAAATAGGTATTGGTTTATTGCTTTACCTGAAAATAGCTTTAACTGGTTATCGTTTTTTGGTTCTTTCTTTATAACCTTTTTTGTTTTTGAATTTATTAGTTGA", "aa": "MQTPNNFPNWLNDLPDRYKFSINGLTFAILGIGESVETGYPVLSLQSPLPNTQDEALIFVNDQAYRSILFAVPAANQENYYAFKSTDLKQHTDQDPVQFIANRLEGYLDVPRSDLAFNVKDISKFNYLTTARNYFPDLVQSYLAIVSTVIAIFLIILALYLIILLIKSFIKKNQTEFSIIRAGGFSTTKFIVGMSVFAGIVAIVSSFLGVLFAFLLEGQVKGIINRYWFIALPENSFNWLSFFGSFFITFFVFEFIS", "aa_hexdigest": "3a8d471eaf58702ca7def26266c0fc43", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 108, "aa_cov": 0.42023346303501946, "hmm_cov": 0.9814814814814815, "evalue": 5.026523550964881e-11, "score": 37.818782806396484, "start": 149, "stop": 256 } ], "seq_stats": { "molecular_weight": 29132.26780000003, "isoelectric_point": 5.189138603210448 }, "id": "KKNAAMEMLJ_1024", "locus": "GCA000027325_01024" }, { "type": "cds", "sequence": "contig_1", "start": 574042, "stop": 574212, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAGATCCCTATTACTGTTAAAAATGGGGTCAATCCATTAATATTACCTACTGATCAACAAAATTTAGTTGTGCAAACCCCTGAAGCACACGGAGCGGTTGTTTCTCAACAATGGTTGTTTAGACACAATAAAGAAATTCTTCCCCAGGAAGGGGAATATGCTTGA", "aa": "MLEIPITVKNGVNPLILPTDQQNLVVQTPEAHGAVVSQQWLFRHNKEILPQEGEYA", "aa_hexdigest": "de0f0ed0d6112401acaecc8ac71561fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6305.134699999999, "isoelectric_point": 5.013165092468261 }, "id": "KKNAAMEMLJ_1025", "locus": "GCA000027325_01025" }, { "type": "cds", "sequence": "contig_1", "start": 574351, "stop": 574500, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGCTAGTTTAAAAAATACCCAAGCTTTAAGTCCAAAATCTACTAAATTTCCCATTTATCCAAAGTTAGCAAATATTATTGCTCAAGCCCAATTACCAGAAGCTACTAATATCCCAACTACTGCTTTAGATGCTTTAAAACAATGA", "aa": "MTASLKNTQALSPKSTKFPIYPKLANIIAQAQLPEATNIPTTALDALKQ", "aa_hexdigest": "bcf5b4d866410aac7ef0c2d45bb43c5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5253.076300000001, "isoelectric_point": 9.699606895446777 }, "id": "KKNAAMEMLJ_1026", "locus": "GCA000027325_01026" }, { "type": "cds", "sequence": "contig_1", "start": 574855, "stop": 575058, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGTAAGAAATAATAATCTTTCTTTTCAACCTTCAAGCAACAATCTCCAATTAGTTCAAAATCAAAACAGTCAAGCAAGTAATGGAATAGCTGATCCTAACTTTTCAAATGTTCAAACTGCATACAATAAAATTCACCAGTCCAATAACACTCCTGAAAAAACCCTTGATGCAGTTTATGCAGCAGTACTTGATCAATGA", "aa": "MMVRNNNLSFQPSSNNLQLVQNQNSQASNGIADPNFSNVQTAYNKIHQSNNTPEKTLDAVYAAVLDQ", "aa_hexdigest": "210297acbff90a9b93b828ac5725b3b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7408.989800000003, "isoelectric_point": 5.4106969833374015 }, "id": "KKNAAMEMLJ_1027", "locus": "GCA000027325_01027" }, { "type": "cds", "sequence": "contig_1", "start": 575081, "stop": 575341, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTTTATCACTATCACTAAAACCTGGTAAGGAATTGAGAATTGTATCTTTCTCAGATTCAACTTTCTTTTTAAGTCAATCTTTATTAATTTTTAGTTCAAATTTCTTAAGTTGCTTAAGTTTATTTTCAAGATCTCAATCTCCAAAAGAGGAATTATTGGCTATAGTTTTAGCATTAGTTTCTAACCACTTTAACTGTTCATTATCTAGCTTATTTAATTTAGAAATATCTAAATTAAAAGTTAATGTAAAACTTTGA", "aa": "MSLSLSLKPGKELRIVSFSDSTFFLSQSLLIFSSNFLSCLSLFSRSQSPKEELLAIVLALVSNHFNCSLSSLFNLEISKLKVNVKL", "aa_hexdigest": "7c143e4416dd000689cf7bf59f10e6e2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9544.11399999999, "isoelectric_point": 9.184117698669432 }, "id": "KKNAAMEMLJ_1028", "locus": "GCA000027325_01028" }, { "type": "cds", "sequence": "contig_1", "start": 575470, "stop": 576345, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCTTTTTTTAAACAGATATTTAAATCGCTTAAAAAGTTCTTCTTTTTATTATTTGGAATTATCTTTGTTTTATTTAGCATTATTTTCCTTGAAACATCTATTGTTCAGTTAAGTAACAACTTAGTTAGTACCTACACTACATTAGTATCTAAAACTAACTCTTCTGATATAGTAGCTCCTGCTATTTTAAAGGAAGCTAATCCTGTTTATATAGCCAGTTTAACTAATGATAGTGGTTATTTTTCCAAAATAAAGATAGATGATAAAAAAATTAACTATCTTTTTCCTTATCAAGAAAATGATTTTGGAAGTGATTCAGGACAAAGTAATGGTAGTGGTGATAACCAAAACAAAACCATTCCCCGCAAAGGCGATGTTAATGAAAAAGATAAACTCTTTTTAGCAAGAAAAAGAGGTATTTTAAAAGCATATGGAGAAGCCAATATTGCTGAAAAAAGAATTTATAAAGGGTTAGCAGTTTCTTTTAATAATACTGATTCTTTTAATGGTAGTGATATTAGTGATTCCATTACAAATAGACACATAATATCAGACCCTCAAAACCTTATTTATGATGCTAGTGGTAACCTTCTAGGTTATTTTGCTGATGGTTTAATTAAAGAAACTATCTCATTGCGTGCTGGCATTGCGCGTTTTCCTGGTGATAAAGGAAAAAGCACAGGAACACAAGTAAAAATTACACAAAAGCAACAAACAAACAATGATCCACAAAAAGACAGTACTGTTAATTCACTTTATAAAACCAATAACAAAGATAAAGTTTGGTTTAAGAGTGATGAGACCAAAGCAGATAATACTGATATATCTGCTAACTATCTATTTACAGGTGGTAATGAAGCTGCTAACTGA", "aa": "MFSFFKQIFKSLKKFFFLLFGIIFVLFSIIFLETSIVQLSNNLVSTYTTLVSKTNSSDIVAPAILKEANPVYIASLTNDSGYFSKIKIDDKKINYLFPYQENDFGSDSGQSNGSGDNQNKTIPRKGDVNEKDKLFLARKRGILKAYGEANIAEKRIYKGLAVSFNNTDSFNGSDISDSITNRHIISDPQNLIYDASGNLLGYFADGLIKETISLRAGIARFPGDKGKSTGTQVKITQKQQTNNDPQKDSTVNSLYKTNNKDKVWFKSDETKADNTDISANYLFTGGNEAAN", "aa_hexdigest": "b1172e505ade3b7679e11e304c2fe200", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 32349.930600000014, "isoelectric_point": 9.046026420593261 }, "id": "KKNAAMEMLJ_1029", "locus": "GCA000027325_01029" }, { "type": "cds", "sequence": "contig_1", "start": 576357, "stop": 577205, "strand": "-", "frame": 1, "gene": "lolD", "product": "Putative ABC transporter ATP-binding protein MG468-1", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_Q50316", "UniRef:UniRef90_Q9ZB70" ], "nt": "ATGGTTTTAAAAACAAAAGAGAACAAAAAGTTTGATATTTACTTAAAATCATCAGATTTTGCTGTTAGTAAAAAAGCATCTAAATTAATTAAAAAGTTAAATAAAAAGCACCCCAAAAGAAAATCACTTAATTCATTTGAAGCTAAAAAGTATGATATTTACTTCAAAGAAGTTTGTAAAGCTGTTACAAATGGCATTAACAACCAACTTATTTGTAACCACATTAATTTAAAAATTCTTCCAGGAGAATTTGTTGTTATTCTTGGTAAATCTGGTAGTGGTAAAACCAGCTTACTTTCTTTAATTTCCGCATTAGATCGACCAACTAGCGGCGATAGTTTTGTTTGTGGAACTAACACCATATGTTGTAGTGATGCAAAACTAACCGCGTTAAGAAATAAAAACGTTGGTTATATTTTTCAACAATATGGTTTATTGCGTGATCTTGACGTTGATGATAATATTAAGCTTGCTCTACCATTAAAAAAAAGGTTTAATAATAATCTTGAAGAATTATTAGAACGCTTGGAATTAAAAGAACATAGACATAAGAAAGTTCATAAACTCTCTGGTGGACAACAACAACGTGTTGCTATTGCTCGGGCTTTAATTAAAGAACCTAAAATTTTGTTTGGTGATGAACCTACTGGAGCAGTTAATATTGATATTTCCAAAAAAATATTGCAATTTTTTGTGGAATATAATCGTGACAAAGGTACTACTATAGTAATAGTTACCCACAACGAAAAGATAGTGGAATTAGCTAAACGGGTAATTAAAATCCATGATGGCAAGATAATCGTTGATTATTTAAATCAGAATCCAAAAACCATCGAACAAATTAACTGA", "aa": "MVLKTKENKKFDIYLKSSDFAVSKKASKLIKKLNKKHPKRKSLNSFEAKKYDIYFKEVCKAVTNGINNQLICNHINLKILPGEFVVILGKSGSGKTSLLSLISALDRPTSGDSFVCGTNTICCSDAKLTALRNKNVGYIFQQYGLLRDLDVDDNIKLALPLKKRFNNNLEELLERLELKEHRHKKVHKLSGGQQQRVAIARALIKEPKILFGDEPTGAVNIDISKKILQFFVEYNRDKGTTIVIVTHNEKIVELAKRVIKIHDGKIIVDYLNQNPKTIEQIN", "aa_hexdigest": "ef08384f9e865f7a80c59c3d5d757ed0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9ZB70", "query_cov": 1.0, "subject_cov": 0.9929577464788732, "identity": 1.0, "score": 542.0, "evalue": 1.64e-194, "valid": true, "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG468.1", "uniref50_id": "UniRef50_Q50316", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q50316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50316" ], "product": "Putative ABC transporter ATP-binding protein MG468.1 homolog" }, "genes": [ "lolD" ], "id": "KKNAAMEMLJ_1030", "locus": "GCA000027325_01030" }, { "type": "cds", "sequence": "contig_1", "start": 577268, "stop": 578581, "strand": "-", "frame": 2, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "GO:0003688", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006260", "GO:0006270", "GO:0006275", "GO:0016887", "KEGG:K02313", "RefSeq:WP_010869493.1", "SO:0001217", "UniParc:UPI000012952C", "UniRef:UniRef100_P35888", "UniRef:UniRef50_P35888", "UniRef:UniRef90_P35888" ], "nt": "ATGGAACAATTTAATGCCTTTAAATCTCTATTAAAAAAGCATTATGAAAAAACAATAGGTTTTCATGATAAATACATTAAAGACATTAATCGTTTCGTATTTAAAAATAATGTTCTTTTAATTCTTTTAGAAAATGAATTTGCTCGTAATTCCTTAAATGATAATTCTGAAATTATTCATTTAGCTGAAAGTTTGTATGAAGGAATTAAAAGTGTTAATTTTGTTAATGAGCAAGATTTCTTTTTTAACTTAGCAAAATTAGAAGAAAATAGTCGTGATACTCTTTATCAAAATTCTGGATTGAGTAAAAACTATACTTTTCAAAACTTTGTAATTAGTGAAGGAAATAAAAGAGCTTATGAAGCAGGCGTTAGATTAGCTGAAACTCAAGATAACGAATTTTCACCGCTTTTTATTTACGGAGAAACCGGTCTTGGTAAAACTCACCTACTACAAGCAATAGGAAATGAAAAATTTCGTAATTTTCCAAATGCCAGAGTAAAGTATGTTGTTTCAAGTGATTTTGCCCAAGAAGTTGTTGATGCTTTTTATCAAAGGGATAAAGGTATAGAAAAACTAAAAAAAAATTATGAAAATTTAGATTTAGTTTTAATAGATGACACTCAAATATTTGGCAGAAAAGAAAAAACCTTAGAAATTCTTTTCAATATTTTTAATAACCTAGTTCTAAATAAAAAACAAATTGTTTTAGTTTCTGATAAGGCTCCTGATGAACTAATTGATATTGATGCAAGAATGATTTCTCGCTTTAAATCAGGATTATTACTAAAGATAGAAAAGCATAATTTGTCTTCACTTTGTGAAATACTTACTGTTAAATTAAAAGAAAAAGATCCTAACATCCAAATAACTAATGAGGCAAGACATGATGCAGCACAAATTTCAGGTAACGATGTGCGTGCTTTAAATGGAATTGCAACAAAGTTATTATTTTTTGCTAAAACTTCAAAACAAAATTTAATAAATACTGAAAATTTAAAAGAAATTCTTTTTGAAGAATTTGAGAAGTTTCATAAAAAGAGCTTTGATCCTTATTTATTAATAGAGAACGTTTGCCGTAGATTTAATGTTCCTATGGACAGTGTACTTTCAGAAAATCGTAAAGCAGAACTTGTCCGTGTTCGTGATGTGTGTAATTACCTTTTGCGTCAAAAGTACAACATGCAATTTCAACAAATTGGCAAAATATTTAAGAGAAGTCATTCAAGTGTATTAATGGCAGTTAAAAGAGTTGCTAAAATGATTGAAAATGACAGTTCATTACGGGATGTAATTACATCATTAGTAATTTAG", "aa": "MEQFNAFKSLLKKHYEKTIGFHDKYIKDINRFVFKNNVLLILLENEFARNSLNDNSEIIHLAESLYEGIKSVNFVNEQDFFFNLAKLEENSRDTLYQNSGLSKNYTFQNFVISEGNKRAYEAGVRLAETQDNEFSPLFIYGETGLGKTHLLQAIGNEKFRNFPNARVKYVVSSDFAQEVVDAFYQRDKGIEKLKKNYENLDLVLIDDTQIFGRKEKTLEILFNIFNNLVLNKKQIVLVSDKAPDELIDIDARMISRFKSGLLLKIEKHNLSSLCEILTVKLKEKDPNIQITNEARHDAAQISGNDVRALNGIATKLLFFAKTSKQNLINTENLKEILFEEFEKFHKKSFDPYLLIENVCRRFNVPMDSVLSENRKAELVRVRDVCNYLLRQKYNMQFQQIGKIFKRSHSSVLMAVKRVAKMIENDSSLRDVITSLVI", "aa_hexdigest": "78cb41d1b32b566578d71be19e91d6ce", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012952C", "ncbi_nrp_id": "WP_010869493.1", "uniref100_id": "UniRef100_P35888", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012952C", "RefSeq:WP_010869493.1", "UniRef:UniRef100_P35888" ] }, "ips": { "uniref100_id": "UniRef100_P35888", "uniref90_id": "UniRef90_P35888" }, "psc": { "uniref90_id": "UniRef90_P35888", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_P35888", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "go_ids": [ "GO:0005737", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0003688", "GO:0006260", "GO:0006270", "GO:0006275" ] }, "pscc": { "uniref50_id": "UniRef50_P35888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35888" ], "product": "Chromosomal replication initiator protein DnaA" }, "genes": [ "dnaA" ], "id": "KKNAAMEMLJ_1031", "locus": "GCA000027325_01031" }, { "type": "oriC", "sequence": "contig_1", "start": 578582, "stop": 579223, "strand": "?", "product": "origin of replication", "nt": "TATTCTTCTATAACATTGTCAAGAATGATAGTTAAAATTCTCGAAATTGGGATATTAACTGCTTTGGAGTAATTTCTAACTTTTTGTCATACTCTTTGACTTGTATAGAAGTGTACACCTGTATCTAGTTTTTCTTGGCGTTCAACAGGAACTATTCCTGGTATTTTTGTTTTAGGTTGGGGAGGAATAGGCTGTGGTTGTGTGAATTGTTGTTGAAAATTTTGATTTTTTTGCTGTAAGAAACCATTATTATGATATTGAAAATTTTGTTCCTCTTGAAAATATCTCTCTTTTTTTGGTTTTCCAGAAAAATTTGATGAAAAAGATTTTTCTTCATTTCAATTTTCAAGATTATTTTCATTTTGTTGATTTATTTGCTCAGGCTGTTGAAATGAATTATTTTTTGATCAAAAAGATTTTGGAAAGGTTTTTTCAAAAGCAGATAAAGGTCCAAAATCAAATGAAGATGAATCTTTGTCAAAAGATGTTTCTTCTCTTTTTGACAAATTTTGTTTTTGATTAAACTTATTTTTATTTTGGGGTGTTACTTTTTCTTTTATGGAAAACAAATCTTCTTCTAAAAGACTTTGTTCTGGGTCATCATCTTGTGCTAAATCAAAGAAAAAACGTTTCTTTTTGTTA", "id": "KKNAAMEMLJ_1034" }, { "type": "cds", "sequence": "contig_1", "start": 578989, "stop": 579234, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCATTAATAACAAAAAGAAACGTTTTTTCTTTGATTTAGCACAAGATGATGACCCAGAACAAAGTCTTTTAGAAGAAGATTTGTTTTCCATAAAAGAAAAAGTAACACCCCAAAATAAAAATAAGTTTAATCAAAAACAAAATTTGTCAAAAAGAGAAGAAACATCTTTTGACAAAGATTCATCTTCATTTGATTTTGGACCTTTATCTGCTTTTGAAAAAACCTTTCCAAAATCTTTTTGA", "aa": "MSINNKKKRFFFDLAQDDDPEQSLLEEDLFSIKEKVTPQNKNKFNQKQNLSKREETSFDKDSSSFDFGPLSAFEKTFPKSF", "aa_hexdigest": "a134ba9314829478f9d26a2092170a99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9534.465299999993, "isoelectric_point": 5.4164945602416985 }, "id": "KKNAAMEMLJ_1032", "locus": "GCA000027325_01032" }, { "type": "cds", "sequence": "contig_1", "start": 579224, "stop": 580033, "strand": "-", "frame": 2, "gene": "parA", "product": "ParA family protein MG470", "db_xrefs": [ "COG:COG1192", "COG:DN", "KEGG:K03496", "RefSeq:WP_009885563.1", "SO:0001217", "UniParc:UPI000013131F", "UniRef:UniRef100_P47706", "UniRef:UniRef50_P47706", "UniRef:UniRef90_P47706" ], "nt": "ATGATCATTTCTTTTGTAAATAATAAAGGCGGCGTATTAAAAACTACAATGGCAACTAATGTTGCAGGATCATTGGTTAAACTTTGTCCAGAAAGGCGAAAAGTCATTCTTGATCTTGACGGGCAGGGGAATGTTTCTGCATCATTTGGACAAAACCCTGAAAGATTGAATAACACGTTAATTGACATTTTGTTGAAGGTTCCAAAATTCAGTGGTTCAAATAATTTCATTGAAATAGATGATTGCTTGCTATCTGTATATGAGGGACTTGATATTCTTCCTTGTAATTTTGAACTTAATTTTGCTGATATAGACATATCACGTAAAAAATATAAGGCCTCAGATATAGCAGAAATAGTTAAGCAACTAGCAAAAAGATATGAATTTGTTTTATTAGATACACCACCAAATATGGCAACTTTAGTTTCTACTGCAATGAGTTTATCAGATGTCATAGTTATTCCCTTTGAACCAGATCAATATTCGATGCTAGGTTTGATGAGAATAGTTGAAACAATAGATACATTTAAGGAAAAAAATACAAATCTTAAAACTATTTTAGTTCCTACAAAAGTTAACGTTCGTACTCGTCTTCATAATGAAGTAATAGATTTAGCTAAAACAAAAGCTAAAAAAAATAATGTAGCTTTTTCTAAAAATTTTGTTTCTCTGACATCAAAATCATCAGCAGCTGTAGGTTATGAAAAATTGCCAATATCATTAGTATCATCACCAAGTAAAAAATATCTAAATGAGTATTTAGAAATAACAAAAGAAATCCTAAATTTAGCAAATTACAATGTCCATTAA", "aa": "MIISFVNNKGGVLKTTMATNVAGSLVKLCPERRKVILDLDGQGNVSASFGQNPERLNNTLIDILLKVPKFSGSNNFIEIDDCLLSVYEGLDILPCNFELNFADIDISRKKYKASDIAEIVKQLAKRYEFVLLDTPPNMATLVSTAMSLSDVIVIPFEPDQYSMLGLMRIVETIDTFKEKNTNLKTILVPTKVNVRTRLHNEVIDLAKTKAKKNNVAFSKNFVSLTSKSSAAVGYEKLPISLVSSPSKKYLNEYLEITKEILNLANYNVH", "aa_hexdigest": "e8f293204ff28c39358c46de4a80ed83", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013131F", "ncbi_nrp_id": "WP_009885563.1", "uniref100_id": "UniRef100_P47706", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013131F", "RefSeq:WP_009885563.1", "UniRef:UniRef100_P47706" ] }, "ips": { "uniref100_id": "UniRef100_P47706", "uniref90_id": "UniRef90_P47706" }, "psc": { "uniref90_id": "UniRef90_P47706", "gene": "ParA", "product": "ParA family protein MG470", "uniref50_id": "UniRef50_P47706", "cog_id": "COG1192", "cog_category": "DN", "kegg_orthology_id": "K03496" }, "pscc": { "uniref50_id": "UniRef50_P47706", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47706" ], "product": "ParA family protein MG470" }, "genes": [ "parA" ], "id": "KKNAAMEMLJ_1033", "locus": "GCA000027325_01033" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Mycoplasmoides genitalium] [strain=G-37] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TAAGTTATTATTTAGTTAATACTTTTAACAATATTATTAAGGTATTTAAAAAATACTATTATAGTATTTAACATAGTTAAATACCTTCCTTAATACTGTTAAATTATATTCAATCAATACATATATAATATTATTAAAATACTTGATAAGTATTATTTAGATATTAGACAAATACTAATTTTATATTGCTTTAATACTTAATAAATACTACTTATGTATTAAGTAAATATTACTGTAATACTAATAACAATATTATTACAATATGCTAGAATAATATTGCTAGTATCAATAATTACTAATATAGTATTAGGAAAATACCATAATAATATTTCTACATAATACTAAGTTAATACTATGTGTAGAATAATAAATAATCAGATTAAAAAAATTTTATTTATCTGAAACATATTTAATCAATTGAACTGATTATTTTCAGCAGTAATAATTACATATGTACATAGTACATATGTAAAATATCATTAATTTCTGTTATATATAATAGTATCTATTTTAGAGAGTATTAATTATTACTATAATTAAGCATTTATGCTTAATTATAAGCTTTTTATGAACAAAATTATAGACATTTTAGTTCTTATAATAAATAATAGATATTAAAGAAAATAAAAAAATAGAAATAAATATCATAACCCTTGATAACCCAGAAATTAATACTTAATCAAAAATGAAAATATTAATTAATAAAAGTGAATTGAATAAAATTTTGAAAAAAATGAATAACGTTATTATTTCCAATAACAAAATAAAACCACATCATTCATATTTTTTAATAGAGGCAAAAGAAAAAGAAATAAACTTTTATGCTAACAATGAATACTTTTCTGTCAAATGTAATTTAAATAAAAATATTGATATTCTTGAACAAGGCTCCTTAATTGTTAAAGGAAAAATTTTTAACGATCTTATTAATGGCATAAAAGAAGAGATTATTACTATTCAAGAAAAAGATCAAACACTTTTGGTTAAAACAAAAAAAACAAGTATTAATTTAAACACAATTAATGTGAATGAATTTCCAAGAATAAGGTTTAATGAAAAAAACGATTTAAGTGAATTTAATCAATTCAAAATAAATTATTCACTTTTAGTAAAAGGCATTAAAAAAATTTTTCACTCAGTTTCAAATAATCGTGAAATATCTTCTAAATTTAATGGAGTAAATTTCAATGGATCCAATGGAAAAGAAATATTTTTAGAAGCTTCTGACACTTATAAACTATCTGTTTTTGAGATAAAGCAAGAAACAGAACCATTTGATTTCATTTTGGAGAGTAATTTACTTAGTTTCATTAATTCTTTTAATCCTGAAGAAGATAAATCTATTGTTTTTTATTACAGAAAAGATAATAAAGATAGCTTTAGTACAGAAATGTTGATTTCAATGGATAACTTTATGATTAGTTACACATCGGTTAATGAAAAATTTCCAGAGGTAAACTACTTTTTTGAATTTGAACCTGAAACTAAAATAGTTGTTCAAAAAAATGAATTAAAAGATGCACTTCAAAGAATTCAAACTTTGGCTCAAAATGAAAGAACTTTTTTATGCGATATGCAAATTAACAGTTCTGAATTAAAAATAAGAGCTATTGTTAATAATATCGGAAATTCTCTTGAGGAAATTTCTTGTCTTAAATTTGAAGGTTATAAACTTAATATTTCTTTTAACCCAAGTTCTCTATTAGATCACATAGAGTCTTTTGAATCAAATGAAATAAATTTTGATTTCCAAGGAAATAGTAAGTATTTTTTGATAACCTCTAAAAGTGAACCTGAACTTAAGCAAATATTGGTTCCTTCAAGATAATGAATCTTTACGATCTTTTAGAACTACCAACTACAGCATCAATAAAAGAAATAAAAATTGCTTATAAAAGATTAGCAAAGCGTTATCACCCTGATGTAAATAAATTAGGTTCGCAAACTTTTGTTGAAATTAATAATGCTTATTCAATATTAAGTGATCCTAACCAAAAGGAAAAATATGATTCAATGCTGAAAGTTAATGATTTTCAAAATCGCATCAAAAATTTAGATATTAGTGTTAGATGACATGAAAATTTCATGGAAGAACTCGAACTTCGTAAGAACTGAGAATTTGATTTTTTTTCATCTGATGAAGATTTCTTTTATTCTCCATTTACAAAAAACAAATATGCTTCCTTTTTAGATAAAGATGTTTCTTTAGCTTTTTTTCAGCTTTACAGCAAGGGCAAAATAGATCATCAATTGGAAAAATCTTTATTGAAAAGAAGAGATGTAAAAGAAGCTTGTCAACAGAATAAAAATTTTATTGAAGTTATAAAAGAGCAATATAACTATTTTGGTTGAATTGAAGCTAAGCGTTATTTCAATATTAATGTTGAACTTGAGCTCACACAGAGAGAGATAAGAGATAGAGATGTTGTTAACCTACCTTTAAAAATTAAAGTTATTAATAATGATTTTCCAAATCAACTCTGATATGAAATTTATAAAAACTATTCATTTCGCTTATCTTGAGATATAAAAAATGGTGAAATTGCTGAATTTTTCAATAAAGGTAATAGAGCTTTAGGATGAAAAGGTGACTTAATTGTCAGAATGAAAGTAGTTAATAAAGTAAACAAAAGACTGCGTATTTTTTCAAGCTTTTTTGAGAACGATAAATCTAAATTATGGTTCCTTGTTCCAAACGATAAACAAAGTAATCCTAATAAGGGCGTTTTTAACTATAAAACTCAGCACTTTATTGATTAAAAAACCTTTCATTTTTAATGTGTTATAATTATTTGTTATGCCATAAATTTAGTTTGTGGCAAAAGCTTCTGTACTGTTTATTTAATGGAAGAAAATAACAAAGCAAATATCTATGACTCTAGTAGCATTAAGGTCCTTGAAGGACTTGAGGCTGTTAGAAAACGCCCTGGAATGTACATTGGTTCTACTGGCGAAGAAGGTTTGCATCACATGATCTGAGAGATAGTAGACAACTCAATTGATGAAGCAATGGGAGGTTTTGCCAGTTTTGTTAAGCTTACCCTTGAAGATAATTTTGTTACCCGTGTAGAGGATGATGGAAGAGGGATACCTGTTGATATCCATCCTAAGACTAATCGTTCTACAGTTGAAACAGTTTTTACAGTTCTACACGCTGGCGGTAAATTTGATAACGATAGCTATAAAGTGTCAGGTGGTTTACACGGTGTTGGTGCATCAGTTGTTAATGCGCTTAGTTCTTCTTTTAAAGTTTGAGTTTTTCGTCAAAATAAAAAGTATTTTCTCAGCTTTAGCGATGGAGGAAAGGTAATTGGAGATTTGGTCCAAGAAGGTAACTCTGAAAAAGAGCATGGAACAATTGTTGAGTTTGTTCCTGATTTCTCTGTAATGGAAAAGAGTGATTACAAACAAACTGTAATTGTAAGCAGACTCCAGCAATTAGCTTTTTTAAACAAGGGAATAAGAATTGACTTTGTTGATAATCGTAAACAAAACCCACAGTCTTTTTCTTGAAAATATGATGGGGGATTGGTTGAATATATCCACCACCTAAACAACGAAAAAGAACCACTTTTTAATGAAGTTATTGCTGATGAAAAAACTGAAACTGTAAAAGCTGTTAATCGTGATGAAAACTACACAGTAAAGGTTGAAGTTGCTTTTCAATATAACAAAACATACAACCAATCAATTTTCAGTTTTTGTAACAACATTAATACTACAGAAGGTGGAACCCATGTGGAAGGTTTTCGTAATGCACTTGTTAAGATCATTAATCGCTTTGCTGTTGAAAATAAATTCCTAAAAGATAGTGATGAAAAGATTAACCGTGATGATGTTTGTGAAGGATTAACTGCTATTATTTCCATTAAACACCCAAACCCACAATATGAAGGACAAACTAAAAAGAAGTTAGGTAATACTGAGGTAAGACCTTTAGTTAATAGTGTTGTTAGTGAAATCTTTGAACGCTTCATGTTAGAAAACCCACAAGAAGCAAACGCTATCATCAGAAAAACACTTTTAGCTCAAGAAGCGAGAAGAAGAAGTCAAGAGGCTAGGGAGTTAACTCGTCGTAAATCACCTTTTGATAGTGGTTCATTACCAGGTAAATTAGCTGATTGTACAACCAGAGATCCTTCGATTAGTGAACTTTACATTGTTGAGGGTGATAGTGCTGGTGGCACTGCTAAAACAGGAAGAGATCGTTATTTTCAAGCTATCTTACCCTTAAGAGGAAAGATTTTAAACGTTGAAAAATCTAACTTTGAACAAATCTTTAATAATGCAGAAATTTCTGCATTAGTGATGGCAATAGGCTGTGGGATTAAACCTGATTTTGAACTTGAAAAACTTAGATATAGCAAGATTGTGATCATGACAGATGCTGATGTTGATGGTGCACACATAAGAACACTTCTCTTAACTTTCTTTTTTCGCTTTATGTATCCTTTGGTTGAACAAGGCAATATTTTTATTGCTCAACCCCCACTTTATAAAGTGTCATATTCCCATAAGGATTTATACATGCACACTGATGTTCAACTTGAACAGTGAAAAAGTCAAAACCCTAACGTAAAGTTTGGGTTACAAAGATATAAAGGACTTGGAGAAATGGATGCATTGCAGCTGTGAGAAACAACAATGGATCCTAAGGTTAGAACATTGTTAAAAGTTACTGTTGAAGATGCTTCTATTGCTGATAAAGCTTTTTCACTGTTGATGGGTGATGAAGTTCCCCCAAGAAGAGAATTTATTGAAAAAAATGCTCGTAGTGTTAAAAACATTGATATTTAATTTGGTTAGTATAAATGGCAAAGCAACAAGATCAAGTAGATAAGATTCGTGAAAACTTAGACAATTCAACTGTCAAAAGTATTTCATTAGCAAATGAACTTGAGCGTTCATTCATGGAATATGCTATGTCAGTTATTGTTGCTCGTGCTTTACCTGATGCTAGAGATGGACTTAAACCAGTTCATCGTCGTGTTCTTTATGGTGCTTATATTGGTGGCATGCACCATGATCGTCCTTTTAAAAAGTCTGCGAGGATTGTTGGTGATGTAATGAGTAAATTCCACCCTCATGGTGATATGGCAATATATGACACCATGTCAAGAATGGCTCAAGACTTTTCATTAAGATACCTTTTAATTGATGGTCATGGTAATTTTGGTTCTATAGATGGTGATAGACCTGCTGCACAACGTTATACAGAAGCAAGATTATCTAAACTTGCAGCAGAACTTTTAAAAGATATTGATAAAGATACAGTTGACTTTATTGCTAATTATGATGGTGAGGAAAAAGAACCAACTGTTCTACCAGCAGCTTTCCCTAACTTACTTGCAAATGGTTCTAGTGGGATTGCAGTTGGAATGTCAACATCTATTCCTTCCCATAATCTCTCTGAATTAATTGCGGGTTTAATCATGTTAATTGATAATCCTCAATGCACTTTTCAAGAATTATTAACTGTAATTAAAGGACCTGATTTTCCAACAGGAGCTAACATTATCTACACAAAAGGAATTGAAAGCTACTTTGAAACAGGTAAAGGCAATGTAGTAATTCGTTCTAAAGTTGAGATAGAACAATTGCAAACAAGAAGTGCATTAGTTGTAACTGAAATTCCTTACATGGTTAACAAAACTACCTTAATTGAAAAGATTGTAGAACTTGTTAAAGCTGAAGAGATTTCAGGAATTGCTGATATCCGTGATGAATCCTCTCGAGAAGGAATAAGGTTAGTGATTGAAGTAAAACGCGACACTGTACCTGAAGTTTTATTAAATCAACTTTTTAAATCAACAAGATTACAAGTACGCTTCCCTGTTAATATGCTTGCTTTAGTTAAAGGAGCTCCTGTACTTCTCAACATGAAACAAGCTTTGGAAGTATATCTTGATCATCAAATTGATGTTCTTGTTAGAAAAACAAAGTTTGTGCTTAATAAACAACAAGAACGTTATCACATTTTAAGCGGACTTTTAATTGCTGCTTTAAATATTGATGAGGTTGTTGCAATTATTAAAAAATCAGCAAATAACCAGGAAGCAATTAATACATTAAATACAAAGTTTAAGCTTGATGAAATTCAAGCTAAAGCAGTTCTTGACATGCGTTTAAGGAGCTTAAGCGTACTTGAAGTTAACAAACTTCAAACTGAACAAAAAGAGTTAAAAGATTCAATTGAATTTTGTAAGAAAGTGTTAGCTGATCAAAAATTACAGCTAAAAATAATCAAAGAGGAATTGCAAAAAATCAATGATCAGTTTGGTGATGAAAGAAGAAGTGAAATTCTCTATGATATCTCTGAGGAAATTGATGATGAATCATTGATAAAAGTTGAGAATGTAGTGATAACTATGTCTACAAATGGTTATCTAAAAAGGATTGGAGTTGATGCTTATAATCTTCAACATCGTGGTGGAGTTGGGGTTAAAGGGCTAACTACTTATGTTGATGATAGTATTAGTCAATTATTGGTCTGTTCAACTCACTCTGACTTATTATTTTTTACTGATAAGGGTAAGGTTTATAGAATTAGAGCTCATCAAATTCCCTATGGTTTTAGAACAAATAAAGGTATTCCCGCTGTTAACTTAATCAAAATTGAAAAGGATGAAAGAATTTGTTCATTGTTATCTGTTAATAACTATGATGATGGTTATTTCTTTTTCTGTACTAAAAATGGAATTGTTAAAAGAACGAGCTTGAATGAATTCATCAACATCTTAAGTAATGGTAAGCGGGCTATATCTTTTGATGATAATGACACTTTGTATTCAGTAATTAAAACCCACGGAAATGATGAGATTTTTATTGGTTCTACCAATGGATTTGTTGTTCGCTTCCATGAAAATCAACTCAGAGTTCTTTCAAGAACAGCAAGAGGTGTATTTGGTATCAGTTTAAATAAAGGAGAATTTGTTAATGGACTATCAACTTCAAGCAACGGTAGCTTACTTTTATCAGTCGGTCAAAATGGAATAGGTAAATTAACGAGCATAGATAAATATAGACTCACAAAACGTAATGCTAAGGGAGTTAAAACTCTAAGGGTTACTGATAGAACAGGCCCTGTTGTTACAACAACCACTGTTTTTGGTAATGAGGATCTTTTAATGATTTCCTCTGCTGGTAAAATTGTGCGTACCAGTTTACAAGAACTTTCAGAACAAGGTAAAAACACTTCTGGTGTTAAGTTAATTAGATTAAAAGATAATGAACGTTTAGAAAGAGTAACTATCTTTAAAGAAGAGTTAGAAGACAAAGAAATGCAACTAGAAGATGTTGGATCCAAACAAATTACGCAATAACTATGATTTCTTTAAAAAGAAACTGTTAGAAAGAAATGTAAATGAGCAATTATTAAATCAGTTTATTCAAACTGATAAACTAATGCGCAAAAACTTGCAACAACTTGAACTTGCTAACCAAAAACAAAGCTTGTTGGCAAAACAAGTTGCTAAGCAAAAAGATAATAAAAAGCTATTAGCTGAATCAAAAGAACTTAAGCAGAAGATTGAAAACTTAAATAATGCTTATAAAGATTCACAAAACATTAGTCAAGATTTACTTCTAAATTTTCCTAATATTGCTCATGAATCAGTTCCTGTTGGTAAAAATGAATCAGCAAACTTAGAACTTCTTAAAGAAGGGAGAAAACCAGTTTTTGATTTCAAACCTTTACCACATCGAGAGTTATGTGAAAAGTTAAATTTAGTTGCTTTTGATAAAGCTACTAAGATTAGTGGAACTAGGTTTGTTGCATATACAGATAAAGCAGCTAAACTACTTAGAGCGATAACTAATCTAATGATTGACCTTAATAAAAGCAAGTATCAAGAATGAAACCTGCCAGTTGTTATTAATGAATTAAGTTTAAGATCAACCGGACAACTACCTAAGTTTAAAGATGATGTTTTTAAACTAGAAAACACCCGTTATTATCTTTCTCCAACTTTAGAGGTACAACTTATCAATTTACATGCTAATGAAATTTTTAATGAAGAAGATTTACCTAAATACTACACTGCAACAGGTATTAACTTTCGTCAAGAAGCGGGTAGTGCTGGTAAACAAACCAAAGGAACTATTAGATTGCATCAGTTTCAAAAAACTGAGTTAGTTAAGTTTTGTAAACCTGAAAATGCTATCAATGAATTGGAAGCAATGGTTAGAGATGCTGAACAAATCTTAAAGGCACTTAAGTTACCTTTTAGAAGGTTATTGTTATGTACTGGTGATATGGGCTTTAGTGCTGAAAAAACATATGATCTTGAAGTTTGAATGGCAGCTAGCAATGAATATCGTGAAGTTTCTTCTTGTTCATCTTGTGGTGATTTTCAAGCAAGAAGAGCTATGATTCGTTACAAAGATATTAACAACGGTAAAAACAGTTATGTTGCTACTTTAAATGGAACAGCATTATCTATTGATAGAATTTTTGCTGCAATTCTAGAAAATTTTCAAACAAAAGATGGCAAAATTCTTATCCCACAAGCATTAAAAAAATACCTTGATTTTGACACAATCAAGTAATTTATGAATAAAGGTGTTTTTGTTGTTATTGAAGGAGTTGATGGAGCGGGCAAAACTGCTTTAATTGAAGGTTTTAAAAAACTTTATCCAACTAAGTTTTTGAACTATCAACTTACTTATACTAGAGAACCTGGTGGTACTTTGTTAGCTGAAAAAATTCGTCAACTTCTTTTAAATGAAACAATGGAACCTCTAACTGAAGCTTATTTGTTTGCCGCAGCTAGAACTGAACATATCAGTAAGCTAATTAAACCAGCAATTGAAAAAGAACAACTAGTTATTTCAGATAGATTTGTTTTCTCTAGTTTTGCATACCAAGGATTAAGCAAAAAAATAGGCATTGATACAGTAAAACAGATTAATCATCATGCGTTAAGAAATATGATGCCAAACTTTACCTTTATTTTGGATTGCAATTTTAAAGAAGCATTACAAAGGATGCAAAAGCGTGGTAATGATAATCTTCTTGATGAATTTATTAAAGGAAAGAATGATTTTGATACAGTTCGTTCTTATTATTTAAGCTTAGTTGATAAAAAAAACTGTTTCTTGATTAATGGTGATAATAAACAAGAACACCTAGAGAAATTTATTGAATTGTTAACAAGATGCTTACAACAACCCACGCATTACTAATCATTCAAAGAAAAGGTAGTTTCTTAAAACCTTTTCTTGATAATTATCTTACTAGTATTGTTTGTGAAAACAAAAATGGTTGCAAAAAGTGTATAAACTGTTTGGAAATTCTCAATAATAAATACAACAGCTTATATTGATTTGATCAAATTAATCCTTTCAAAAGAGAAAATGCCCTTCAGTTAGCAAGAATTTTTAACCGTGAAAGAACAAGTGTAAACAATAAAAATATTTATCTAATTGAAGAAATTGAAAAATTAAGCAGTAATTCTATAAATAGTTTATTGAGACTAGTTGAAGATAGTCCGATAAATAGTTATGGTATTTTTACAACTAAAAATGAAAGTTTAATTCTTTCCACTTTTTTAAGTAGAGTACAGAAAGTAGTTTTAAAAAAAGCTAGTAAAGTTCCTTTTAAAGTAAGCAAAAATGATCAAGAAATTATTACAAGTTTTTTTACTGTAGATGAACAAATTGAAGCAATTGAAAATGGAAGTTTTAACCGTTTCAAAATTATCTTAGATGCATGTTTAAACAAAAAAACAGGTACAGAACAAATTTATCATGCTTGACAAATTTTTAGAGATTTTTCTAATAGTGAAATTGCTCAGTTAATTACTCTAATAATTAATAAAACTGAAAATATAGATAAAAAATCAATTTTGTTTAATTGTTTAAAAGTTTTGCCATATAATCCTCCAAAATCCACTTTGTTTGCTAATTTAGTTAGTTGATAGTTATGAAAAGCGAAATTAATATTTTTGCACTAGCAACTGCACCTTTTAATAGTGCATTACATATTATTAGGTTTTCTGGTCCTGATGTTTATGAGATTTTAAACAAGATAACTAATAAAAAAATAACAAGAAAAGGGATGCAAATTCAACGCACATGGATAGTTGATGAAAACAATAAGCGAATTGATGATGTGCTATTATTTAAATTTGTCTCTCCAAATTCTTATACAGGAGAAGATTTAATTGAAATTTCTTGTCATGGTAACATGTTGATCGTTAATGAAATTTGCGCACTTCTTTTAAAAAAAGGAGGTGTTTATGCCAAACCTGGTGAATTTACCCAAAGGAGTTTTTTAAATGGAAAAATGAGTTTACAACAAGCTAGTGCTGTAAATAAATTGATTTTATCTCCTAACTTATTAGTTAAAGATATAGTCTTAAATAATTTAGCGGGTGAAATGGATCAACAATTAGAACAAATAGCTCAACAAGTTAATCAATTAGTAATGCAAATGGAAGTAAACATTGATTATCCAGAATATCTTGATGAACAAGTAGAACTATCAACTTTAAATAATAAAGTTAAATTGATTATTGAAAAGCTTAAAAGAATTATTGAAAATAGTAAACAACTCAAAAAACTTCACGATCCTTTTAAAATTGCCATTATAGGCGAAACTAATGTAGGTAAATCTTCTTTACTCAACGCTTTATTAAATCAAGATAAAGCGATAGTTTCAAATATTAAAGGTAGTACACGCGATGTTGTTGAAGGGGATTTCAATTTAAATGGTTATTTAATCAAGATCTTAGATACTGCAGGTATCCGTAAACATAAAAGTGGGCTTGAAAAAGCAGGAATTAAAAAAAGCTTTGAATCTATAAAGCAAGCTAATTTGGTTATTTATCTTTTAGATGCAACACATCCAAAGAAAGATCTTGAATTAATTAGTTTTTTTAAGAAAAATAAAAAGGATTTTTTTGTTTTCTATAACAAAAAAGATTTAATTACAAATAAGTTTGAAAATAGTATTTCTGCAAAGCAAAAAGATATTAAAGAATTAGTTGATTTATTAACTAAATATATTAACGAGTTTTATAAAAAAATAGATCAAAAAATCTATCTGATTGAAAATTGACAGCAAATTTTAATTGAAAAAATTAAAGAACAATTAGAACAGTTTTTAAAGCAACAAAAAAAATATTTATTTTTCGATGTTTTAGTTACCCATCTAAGAGAAGCTCAACAAGATATTCTTAAACTACTAGGTAAGGATGTAGGTTTTGATTTAGTTAATGAAATTTTTAATAATTTTTGTTTAGGAAAATAATGGAATACTTTGATGCACATTGTCATTTAAATTGTGAACCTTTACTGAGTGAAATTGAAAAAAGCATCGCTAATTTCAAATTAATTAATTTAAAAGCAAATGTTGTAGGTACAGATTTGGATAATTCTAAAATTGCTGTTGAATTAGCTAAAAAATATCCTGATCTTTTAAAAGCAACCATAGGTATCCATCCAAATGATGTTCATTTAGTTGATTTTAAAAAGACAAAAAAACAACTTAATGAACTATTAATAAATAACAGAAATTTCATAAGTTGTATTGGTGAATATGGTTTTGATTATCACTACACAACAGAATTTATTGAATTGCAAAACAAATTCTTTGAGATGCAATTTGAAATAGCTGAAACTAATAAATTGGTTCACATGCTTCATATTCGTGATGCTCATGAAAAAATTTATGAAATATTAACAAGATTAAAGCCAACTCAACCTGTGATTTTTCATTGTTTCAGTCAAGATATAAATATTGCTAAAAAGCTACTATCATTAAAAGATTTAAATATTGACATCTTCTTTTCTATCCCAGGGATAGTTACTTTTAAGAATGCTCAAGCATTACATGAAGCTTTAAAGATTATTCCTAGTGAATTACTTTTAAGTGAAACTGACTCACCGTGATTAACCCCTTCTCCTTTTCGAGGCAAAGTTAACTGACCTGAATATGTAGTTCATACTGTTAGCACTGTTGCTGAAATAAAAAAAATAGAAATTGCTGAAATGAAGCGAATTATTGTTAAAAATGCAAAAAAATTATTTTGACATTAAAAGTTAAATAAAGCAATTTATTTAACAAATGGATGTTAGAACTGAAAGATTAAACGAATTGTTTTTTGTTTATCATAAAAACTTAAAAAATCAATCTAAATCTAAATATAGCAGAGCAGTTAATTACTTAAAAAGGCGTGGATTCAATTTACAAGATTTTTTAAAAGTTGGGGGTGGTGTTGGTTATTTACAAAATAAAGAATGATTAAATTTACCTTTATACAGTTTTGATGGTAATTTAATTGGTTTTCTAAACAGAAAAGTTAGTTATAAAAAGGAATTTTTATATACACCTTTTAATAAACCTCCTTCAAAGAGCGAAGCTTTTGTAGGACTCAGAGAATTAGTTATTAAAGACAATAGCATATATCTTGTTGAAGGTGATTTTGACTGGTTAGCTTTTCGCAAAGCAGGTATATTAAATTCTCTTCCTCTTTGTGGTTTGACTATTTCAAATCAACAAGTTCAATGATTAAAACAAAAAAAGATTAAAAAGATTTTTATCTGTTTTGATAATGATTTAGCTGGAAAGAATGGAGCAAAAAATTTAAAAGAATATCTAACTAAGCAAGGATTTATAACAAAAGTTATAGAAATTAAAGCTGCCGCAAAAGACTGAAATGATTTGTTTTTATTAAACAACTCAAATTGATCAGCGGTTTTAACTAATCAACTTCTTTTTTAAGAATTTTTTAAATTTACTAATAATTGTTCTGATAATTATTTTAGTGATATTTAAATCTGGACAAAGCTGAACTAAAGCTCTCGCACCAGCAGCATCTTCAATTTCATTAACAATAACCCTATTATATCTATTTAAAAAGAAGTCAATAGCATAATAACCTTCCCTTAGGCGTTTAGCTATTTTCTTTATTTTTCTTTTAGTAAATCACTTTAATTTAAACAAGGAAACTTCAGCACCTTGTGAAAAGTTAGCTTTAAATTGATTAGCATTAGAAATTTTTTTAATAACTTTAATTATTTTTCCAAACAAAATATAAGCACGATATTCAACTGTGCCAATTGATAAAAAAGGTTGAACAATTCATTCTGTTGCATTTTCAATGTTTAAATGTTTGATCTCGTCAGCACTATTAACTAAATGTACATCTTTTCCACCGTGTGAATTACGTTTCTTAACGATGACAGGAAATGATTTGATTGTTTCTTTACTAAGAAGAGAAGAATTGACAGTTAGAAATCTATTTTGTTTTAATCATTTATATGTTTCGTATTTATCGTTTGCTATAAAAACAACATTAAAAGGATTAACTAAAAAAGTATTTATTTGATTATTGGTTTTTAAAAAATCTACTTTTCTTGAACGATTTAAAATCAATTCAGCTTCATTAATTTTAGCTTCGAAATTAATGTCTTTATTTTCAAGTAATAAGACTTCAGCATTTAGTTTTTTCTTTTGTAATTCCTTGATTAGACTTAAAGCAAATGTTTGATTTTTTTCAAAATCATCCTTGTTGTCATAAACAACTAATGCTTTTCTGTTTTTTAATTTAATTTTTCCCATTAATCTAAATTGCTTTTAAAAGCTCAATTGCAAGATTAGTATTTAAATACATTGAGCTTCTTGTTAATTGCACATTAGGATTTACTTCACAAAAGATCAATGATCTGTCTTGATCAAACAAAAAATCAATACCGCAATAAAAAAGTTGCATTACTTTACTAATTTTAACTGCTAAATTTTCTTGTTCCTTATTCAAAAAAAAGCGTTCTGCCTTTGCCCCTTTATTGAGATTAGAACGAAAATCACTATTATTAGTTGTATGTAAAGCACCTATAACTTTATTGTTCACAACAATAACTCTTACCGATTCACCTTTAGAGCAAGTAATGTATTTTTGCACAATTGCTTGTTGATTAAAAGACATTAAAGTTTTTCTTAATTTTTGTTCATCTAAACACAAAAAAACATAATCACCAAAAGAACCAAAAACACTTTTAACAATAACAGGAAACTCCATACTTTGTTTTATTTGATTAATAAAAACATCGAGCATTACTGGATTTCACTCCCTGTCAAAATTTTGAGGTCCTAAAAGTGTTTTAATCTGCTTTATGAATGGATATTGTGCAATGATAGCGTGACTAAGTCCTTTATTATCTGCATTGTTAATTCCAATTGAACTGTTAATTACTTCAAAACCATTGCTTTCTAGTCATCTAGCTAAAGCAATATTTTTATCCATAAAAAGAATTTTGTTGGATATGAAGGGTAAATTTGCTTTTGCTTTAGTTGTATTAATATCAAAACTAGTAAAGAATTTTAGTTCTATATCTAACTCTTCACTAGCATTTTTTAAAAGTTGAGTTTGATTTAATTTAGAGCTAATTGGATTAAATGCTGGATTGTAAACAACGTTTATTTTTTTCATGCAATATTCAAAGATTTTTTATTATTGCTAAAACTGTAAAAGGGCCAACACCGCCAGGCGTTTTAGTTCATCATCTAGCTTTTTTTTCAATGCCTTCAGGATTTATATCACCACAAAGTTTATTTTGCTTGTTTCTTGAAACACCTATATCAAATAAAATAACTCCTTTTTTAAAGTTTTTAGCTTGAAAAAAATGAGATTTTCCAATAGCAGTAAAAACTATATCAGCACTTTTAATCAATTCAAATGTATTTGGAGTATCTTTATCGCATGCTTGAACTTTATAACCACTAGTTTTTAAAAATTCATAAATAGGTTTGCCACCAGTTATTCCCAAACCTACCACAACAATTTTTTGATTATTAAATTCAAGGTTAAATAGTTCTTTTAGTGTTATTATAGCTTCAACTATACAAGGTTTAATTTCAGCCAAATGATTAGTTGTTAAACCATCAACATCTTTTAAAGGATTAATTGCTTCTAGTAGTGAGTTTTTATCTAAGTGTGGATAAACTGGCAATTGCAAAATGATACCAGTAACATTTACATCATGATTTAAACTAATTATTTTTTCTATTAACTCTGCTTGAGTTATTTGATTAGATAGTTTAATTAATTCACTTTTTGCTCCTACAGTGTTACAAGCAATAAGTTTTTGTTTAATAAAACTGTCTGATGAATCATCATCATTAGCTTGAATAATCACTAACTTACATTTTGATCAATCAAAATTCTTGTAAGTTTCTAAGATTGATTGCGCTTTTAGTTTTCCATCAAATGACATCTTATTTTGCCTAATATAAAACAAAACGTCTAACTTCTTATTTATGCAAAGGTAAGTTAGACGTTGGAATTCTTTAATGGTGGAGATGAAGGGTCTCAAACCCTCAACCTCCTGAGTGCAAATCAGGTGCTCTATCAGTTGAGCTACATCCCCATTATTGGTGGAAGTAAATGGACTTGAACCATCGACCTCACCCTTATCAGGGGTGTGCTCTAACCAACTGAGCTATACTTCCAAGCATAATCCTAAGGGTATTTAACTAATTATTATAACAATTTTAATTTAACCAAAATACCCCTCGAATTTTAACAGTTTTTATAATCAAAACAGCTAATTTTATGGGGCTTGTTTTAAAAGAATTTAACAATAAAATAAGAACAGCATTAATTCTCGCTCCTTTTTTCACTTTCGCTCAGATAGTGATTGATTTAATCATTCCTTCTTTTTTAGCAAGTGCTATTTCAGTTGTGTTTTCAATTGATAAATTAAAACAGGATGAGTCAGGCGGAAAGACAATTTCAGTTGATTTTATTGGTGGGGCTAATATTAATTTTGCTAATGTAAGAGAAGCGCAAATAGTTTTAGCAACCACAGTTATTTTACTTGCATTATGTGGACTTTTTTTTGGTCTAATATCTATTTATTGTGCAAGTTATGTTTCAGCAAACACTTCTTTTCTATTAAGAAAAAAGATCTTTGCAAAATTAATGCGAATCACAACCCCATCACATGACCATTATGGTTCTTCTACTTTATTAGTTAGATTAACAAATGATGTTTATTTGATGGAGGTAATTGCTTTTGATTTTTTAAGGCTAATTATTCGTGCTCCGCTTTTGTTTATAGGTGGATTAGTGTTTGCAGTAACTACAAATCAAGATATGTCAATATCTTTACTAATTACTTTTCCTTTGATTCTTTTGGTAATTGGTATTCTAAACCGTAAATCTATTCCTTTATTTAAAGAAAATCAAAAATCAGTTGATAAGATAAATGAAAGAGTAGAAGAAGATGTTTCAGGTTATAAAGTAATTCAATCTTTTAATCTTCATTCCTTTACAAATAACAAATTTAAGATTGCAAATGAAGGATGAAAAAAGAATAGTACAAGTTCTTTATTTATAAACTCACTTAACATTCCATTTACCTTTTTTTTAAGCAGTTTAACTATTATTATTGCTCTACTATTAGTTTTTCAATTAGATAGTAGTGTTTCAGTTGACCCATTACCGCAGGATGCAGCTATTAGACCTAATATCTTTGCTTTTTTCCAATATAACTTTTACATTGTTTTAGGGTTTATCTTAACCTCTTTAACAATGGTGAACTTTAATCGTTCTAGAGTTGCACTAGGCAGAATTAAAGATATTTTATCTCAGCCTGAAATAAAAACTATTACAAATAAAGATCAAAAAGAATTATTACCTACCTTGGAGTTTAGAAATATCTCTTTTGGTCTTGGCAATAAGAACAATAACAATTTTTTACAAAATCTTAGTTTTAAATTTGAAGCATATAAAACATATGGTATTGTTGGGCCTACAGGCTCTGGAAAATCTTTAATTGCTAACATTATCGGTGGTTTATATGAGCCTAATGAGGGTGAAATTCTCATTGGTGGGGAGAAAATTCAATCTATTGACAGTTTATATCTATCAGAAATGATAGGAATTGTTTTCCAACAAAACATCCTTTTTAAAGGAACAATTTCTTCTAATATAAAAATTGGTATTGAAACTAGAAGTGATTGAAAGAATCAATCTGATTTACAAAAGAATGAAGCGATGAAAAACGCAGCTAAAATAGCTTGCGCTGACACCTTTATTGAAAAGTTTTCTGATAGTTATGATCACAATGTTGAACAGTTAGGTAAAAACTTATCTGGTGGACAAAAACAAAGAGTTGCTATTGCAAGAACTTTAATTACAAAACCAAGAATTTTAGTTTTTGATGATAGTATGAGTGCTCTTGATGCTCTAACTGAAAAAAAAGTAAGAGAAAATATTGAAAATGATTTAAAGCTAACTACCAAAATCATTATTAGTCAAAACATTAATTCAATTAAACACGCAGATAAAATTTTGGTAATTGATAATGGCAGAATTGTTGGTTTTGATAGTGATCAAAAGCTAATGAAAAATTGTTCACTTTATCAAAAGATGAAAGAATCTCAAAAAGATTTGGGAGGTGATTTTGATGCAGTTAACTAGTGTAAAACCAAGTTCTTGAAAGATTTTAAAGAGATTAATTACTTCAATGGAAGGAAGTTGAAGTTATAAATTACTTTATGTTTTTTTGTGTATAGTTCTTGGTATTTTATATGGAATTGCTAACCCTATCTTATTAGCACAAGGTCTTGGTTTTATTTTTCCTATTACTAGTAGTAATGGTCGTGCTGTTGACTCAATATATTCATTAATTTACCCAACAAATTTAAATGTATTCATTAGGCTCACAATTGTGAGCGTAACTGTTTTTGTAGCTTATGCATTAATCTTTGTATTTAATGTAGCGCAAAACTATGTAGGGATTAAACTTTACCAACAAACATGTGCTACTTTGCGTTGAAAGGCATATTTAAAAATGCAGAGTATGTCAACCAGCTTTTTTGATACGCAAAATAATGGTGATCTTATGAGTAGGTTAACTAATGATATGTATAACATTGATAACCTATTCACTCAAGCTGGTGGACAAGCTATTCAAAGTTTGTTTAATATTTTAACAACCTCAGTATTAATATTTTTATTAAGCCCAGTTATTGCACTTATTTCACTTTCAATTTTAGCTACATTAATTACTTTTTCTTTTGCCTTTCTAAAGAAATCAAAAACTTCATATAGTCAAGTACAAAATAATTTGGGTGATATGTCTGGTTATATTGAAGAGGTTTTAACTAATCATAAGGTTGTTCATGTCTTGAAGTTGCAAGAGATAATGATTAAGGATTTTGATCAATACAACAAATCAATGATCAAACCAACTGTAAGAGGGAATACATATTCGATCTTTCTTTTTTCTTGGTTTGGTTTTATATCAAATATTACTTATCTGGTTTCTATATCAATTGCTACTGCTTTTAGTGTTAATTCTATTCCTTCATTTGGAATTAGTGTTATTAACTATTCATTCATGTTGTCTTACATTGCTTCTTTAAGGCAAATAACTTTAGCATTAGATCAAATCTTTACCCTTTGAAACTTAGTTCAATTAGGGGTTGTTAGTGCAGAAAGAGTATTTAAGGTATTAGATCTTAATGTAGAGAAAGATACTGCTACTATTGACAAATTACCTGATATTAAAGGTAATATAAGGTTTGAAAATGTAGCATTTGGTTACAATAAAGATAAACCTACTTTAACAGGAATTAACTTTAGTGTTAAACATGGAGATGTAGTTGCAATAGTAGGTCCTACAGGAGCTGGTAAATCAACTATTATTAATCTATTGATGAAATTCTATAAACCTTTTGAAGGAAAGATTTATATGGATAACTTTGAAATTAGTGATGTAACTAAAAAAGCATGAAGAGAAAAGATTTCTATAGTATTACAAGATTCATTCTTATTTAGCGGCACAATTAAAGAAAATATTCGTTTAGGCAGACAGGATGCTACTGATGATGAGATTATCGCTGCATGTAAAACTGCTAATGCTCATGATTTCATCATGCGTTTACCAAAAGGATATGACACTTATATTTCCAATAAAGCAGATTATCTTTCTGTTGGTGAAAGGCAATTATTAACAATTGCCAGAGCAGTAATCCGTAATGCTCCAGTTTTGCTCTTAGATGAAGCAACTAGTTCAGTTGATGTCCATTCAGAAAAATTAATTCAAGAATCAATAGGAAGGTTAATGAAAAATAAAACTTCTTTTATAATTTCTCATCGTCTTTCAATTATTCGTGATGCAACATTAATAATGGTTATTAATGATGGTAAAGTACTTGAAATGGGTAATCATGATCAGCTGATGAAACAAAATGGATTTTATGCACGTTTAAAACAATCTTCGGTTCGTTAATAAATTCTAATGACTGTTGCTGAAATTAAAAAACTTGCATTAAATAATCAGGTATTTAATGAAGCAAAAGCGCTTTTAGAAAAAGGTAATGTTATTTTTCCAAAAAAATTCTTAAAGCGAAAGAAGATCATTATTGAAGTATTAGATGGTAAGGTTTTTAAGGTTCAAATTAATTTAAAAACCGCTGCTGCACACTTGGATTGTAGTTGCTCTAATGATAAGCAAAATTGCGTTCATATAATTGCAGCGCTTTTGAAATATAATGATCTAAAAAATCAAGATAACAAAGAATTTGACCTTAATAAAGCCGATAAATTAGAGTGCAAAGAAGTTGAAATTCTAATTGAAAATGTTAGCTTAGCAATTGTTAATGGCAGCTGAAAATTAAAGATAGGTTTTGTAATTAATATTGATAAAGTTCAAACTAATACAACTGCTTTACGTTTTTATTGTTGTGATAATAAAGATGTTTATTTTCTACATACTGAAGATGAACAACTTTTCAGGATTGCTTTAGATAAATTTAATAGTGTTGAAAGACAAACATTATTAATTTTTGATCAGCTAAATAAAACAAAGCAAATGCAATATGAAAATAACAGTTTGCTTTTTAATTTAGATCAATTCCTAAGCCTAGTTAAGGAAGTTAAAAAACCTTCATTATTCTTATTAAATGAGGATAAAACTGACAATATTCTTTTCTTAAGAAGTCAACATAAAATCAATGGATTAAGCCACGTTTGTGGTTTTTTAAATAATAAGGTTTTTGATTTTGTATCCTACAATGAAAAAACTAAGCAAATTGTTTTACGCTTAGCTTATCTTAATAAGTTCACTGATTTTAAATTTCCATACAACATTAACATCTATAAACTTGCTTTTGGAGAAACATTATTTTTCCATTTTTTAATCCATTTAAAAATGAATGGTTTTAAAAACATCTTTTTTCAAAGTGATGTTGTTATTGTTAAGGAAAGTGAATATCTACCTAAGATGTTTTTGACTATTGAATTTAATACACAAAAAAACAAGTTTATAACTGATGCTTTTTTTAAATATAAGAATAAAAATAGCAATACTTTAACAACTGTTTACCCCCATCGCTATTACTTAGCTCAAAAAACTAATACATCAAACTTCAACCGTTTACTTTTTTATGAACAAGCACTACAAAGGTTTTATGAAGAATTATTTCAAATAGATTATTTAAGAAGATTTGAAAACATTCCCATTAAAGATAAGAATCAAATTGCGCTTTTTAAAACTGTTTTTGATGATTACAAAACCATTGATTTAGCAGAATTGAAACTTACAAGTAATCTCTTAAATTACAAACAGTTACATTTTTCAATAAGTGATATTAAAGCTTTAAAAATTGAAGATAGACAACTAAAAATTGAATTTAAAGCTGGCGGGATAGATCTAAAGTTAATTAAAAGTGTTTTAAGTAACTACTATAAGGGTAATGCTATTTGTATTGGTGAAGATGGTTGATATGATTTGAACGATGAAAATGCTAAAGCACTAATTTCATTTTGGAGTCAAATTGACTTAAGAAATGCTACTTGTGATGCTAATAACAATTTGCTTCTTGCTAAATACCACTTGTTTGAAGTTGTAGATACTATTAGTAAATACACTGATGTAACTAATTTATTAGATGAAAAAACAGCCTTACAATTAAAAATAGCTAGTGAAAATCAATTTCATCTTTCGTTAGATAATAACCAAATTAATAACTTACGCAAATATCAAAAAGAAGGAGTTAAATGGATAAGGGCATTAGAAGATAATCAGTTTGGTGGAATTTTAGCAGATGAAATGGGGTTAGGTAAAACTGCTCAAGTGATCTTTGCAATGCTGGATAGTTATCAATCAACAAAATCACTTTTACCTAGTTTAATTATCGTTCCAGCATCCTTACTCTTAAATTGAAAAAGTGAGTTCCAAAAGTTTGCACCTCATGTGAAAATAGTTACTGCTAATGGCAATTTTAAAGAACGCTCGCAGGTATATGAATCATTAAAAAATCAGATTTTGTTAATGAGTTTTAATGTCTTAAGAAGTGATATTAAATGAATATCTCAAAAAAAGTTCCATTATGTAGTAATTGATGAAGCACAAGGAATCAAAAATGAAAATTCTACTGTAACAAAAGCCGCTAAAAAAATTAAAGGTAATTTTTGCTTAGCACTTACCGGTACTCCTATTGAAAATCGTTTGCTTGATCTTTGATCTTGTTTTGATTTTGTTTTACCAAATTTCCTTGGTAATAAAAAACAGTTTTCAGATCAATTTGAAAAAGAAAAAAATGATGAAAGCTTTCAAAAATTAATGAAAAAAACGAGTCCTTTTATTTTAAGAAGGACTAAAAATAAAGTTTTAAAAGAACTACCTAAAAAAATTATTACTGATATCTATGTTGAACTTAGTGAAGAACATCAAAAACTGTATGATAAACAAAAAACAGATGGTTTGAAGGAGATTAAAGAAAGTGATGCTAAAAATGCTTTAAATATCCTTAGTTTAATCTTGAAATTAAGGCATATTTGTAGCTTAGTTAAAGACAATGATGTTAATGATTTTGAAGATAATTCCAAAGCTAATGCAGCTTTAAACATTATTTATGAAGCACTTGAAAATAAACGCAAAGTTATTTTGTTTACTCAATTTTTAGATGTAATTGATTGTTTTAAGCAAACTTTAAAAAATCAAAAGATTGATCACCTGGTATTTGATGGTAGAAAAACTGTGAAGAATAGAAACACTATTATCCAGAAGTTCAATAGTGCTAAAGAACCTTGTGTGATGCTAGCTTCTTTAAAAGCTGGTGGAGTTGGTATTAACTTAACTGCTGCTGAAGTTGTTATTCATTTTGATGTGTGATGAAACAGTGCTGTTGAAAATCAAGCAACTGATAGAGCACATCGTATTGGTCAAAGTAAAACTGTACAAGTTTATAGAATTATTGCTAAAAATACTATTGAAGAGCGAGTTTGTCAAGTTCAGAATCAGAAACAAGAACTTGTTAAAAAAACCTTGGTTGAGGATGTAAATTTCTTTAAATCTCTTTCACATGAAGAACTCTTAAAGCTTTTTGAATAAAGCAAGAATTATAATTAACACTCTAAGGATGCAAGTGATAAATGGCTGCTGGTAAAAGGGATTATTATGAAGTTCTAGGGATATCTAAAAACGCTAGTTCTCAAGACATAAAAAGAGCTTTTAGAAAGCTTGCAATGCAATATCACCCCGATCGTCATAAAGCAGAAAATGAAACTACTCAAAAACAAAATGAGGAAAAGTTTAAAGAGGTTAATGAAGCATATGAAGTTCTAAGTGATGAAGAAAAACGTAAGCTTTATGACCAGTTTGGTCATGAAGGGTTAAATGCTTCTGGTTTTCATGAAGCAGGGTTTAATCCTTTTGACATCTTTAATAGTGTTTTTGGTGAGGGATTTTCCTTTGGAATGGATGGTGATTCACCATTTGATTTCATTTTTAATCGTTCTAAAAAACGTCAACAACAAATTGTTGTTCCCTATAACCTTGATATTGCTTTAGTAATTGAAATTAACTTTTTTGAAATGACTAATGGTTGCAACAAAACCATCAAATATGAAAGAAAAGTTTCATGTCATAGTTGTAATGGTTTTGGCGCTGAAGGCGGGGAAAGTGGATTGGATCTTTGTAAGGATTGTAATGGCAATGGTTTTGTTATTAAAAACCAACGTTCTATCTTTGGAACCATTCAATCCCAAGTCTTGTGTTCAACTTGCAATGGACAAGGAAAACAAATTAAAGTTAAGTGCAAAACTTGTCGTTCTAACAAATACACTGTTACCAATCAAATTAAAGAGATTAATATTCCAGCAGGAATGTATAGTGGTGAAGCTTTAGTTGATGAAAGTGGTGGTAATGAATTTAAAGGTCACTATGGAAAATTAATCATTCAAGTGAATGTATTGGCAAGTAAGATTTTCAAACGTAGTGATAATAATGTTATTGCCAATGTTTTAGTAGATCCAATGGTTGCTATAGTTGGTGGGGTAATTGAACTACCTACTCTTGAAGGGATTAAAGAATTTAATATTAGACCAGGCACTAAGAGTGGCGAACAGATTGTTATTCCTAACGGTGGGATTAAATTCTCAAAGAGTTTTAAAAGAAAAGCTGGGGACTTAATCATTATTATTAGTTATGCACGTCCTTGTGAATACACTAACTTAGAATTGAAAAAATTACGTGAGTTTATCAAACCTAATCAAGAGGTTAAACAATATTTAAATACTTTAAAAAATGAATACAAAACTTAATGTAAAAGGTTATCTAAATGTTGGTGATAACCATCAACTTTATTACTGAACACAAGGAAATCCTAATGGTAAACCGGTTTTGTATATCCATGGCGGACCTGGTTCTGGTACTGATGAAGGATGTTTAAAGTATTTTGATCTTGAAACAACTTGGATTATTTTATTAGATCAAAGAGGTTGTGGTAAGAGTAAGACTAATGATATCTTTTATGAAAATAACACTGATAAATTAGTTAGTGATTTTGAAATTTTACGTCAAAAATTAAACATTAAAAACTGAACACTCTTTGGTGGTAGTTGGGGTTCTGCACTTGCTTTAGTTTATGCAATTAAACACCCACAAGTAGTTGATAAGATCTTTTTAAGAGCACTTTTTTTAGCTAGAGAAAAAGACTGATCTGAAGCTTTAATGGGATTAGGAAAAATGTTTTATCCTTATGAACACCAACGCTTTATGGATAGTATTCCTAAAGCTTATCAGAACAGTTATGAACAAATTGTTAACTACTGTTATGATCAATTTCAAAATGGTGATGAATCAACCAAAGAAAAACTTGCTAAAGCTTGGGTGGATTGAGAATCAACATTACTTTCACCTATTAACAAAATTCATTCAACAGCAACAGACTTTAAATTAGTTGAAAAACTAGCTTTATTGGAATGTCACTATGCAGTTAATAAAAGTTTTTTGGATGAAAACTTCATTCTAGATAACATTAGTGTTCTTAAAAATAAAAGTATTTATTTGGCTCATGGTAGATTTGATCTGATCTGTCCTTTATATCAACCATTAGCATTAAAACAAGCATTCCCTGAATTACAACTTTATGTAACCAATAATGCTGGTCATAGTGGTAGTGATGCTAATAATTTAGCAACTATAAAACACCTTTTAAAAACTTACCTTTAATGAAGCGTTGTTATATTACAACCCCTATCTACTACGCATCAGGTAAGCCACACATAGGTCATGCTTTTACCACTATTTTGGCGGATGTAATTAAGCGTTTTAAAATCCAAAACGGATATGAGGCTTTTTTGCTTGTTGGCAGTGATGAACATGGCAATAAAATAGAAAGTAAAGCTAAAAGTTTAAATTTAGATCCTAAAACATTTGTTGATATTAACGCTCAAGCTTTTAAGTTAATGTGAAAGACCCTTAATATTAGTTTTGATCACTTTATTAGAACAACTGATGAAATCCATAAACAACAAGTTCAAAAAACATTTCAAGATTTATATGACAAAAAACTAATTTATCAAAGTGAATGAAAAGGGGCATATTGTGTTGAGTGTGAACAAAATTACTTTACTTTTAATAAACAAACAATGTTATGTGAAATAGGTCATAATCTCAGTCTTGTCCAAGAACCTTGCTGATTTATTTCTTTTTCTTCTACTAAAAATTGAATTGAAACAACGATAGGAAAAAATCAACTTAACATTATTCCTAAATCACGTGCTTCTGAATTAAAAAATAACTTTATAAACAATGGTTTAAACGATTTAGCATTAACAAGAAAAAATGTTACTTGAGGAATAAAAGTTCCTTTTGATCCAAATCAAACAATCTATGTTTGGTTTGATGCATTGTTTTCTTATATCACCAATTTAGGATTTAGAAATGGTGATCCTAATTTTATAAAGTGATGAAATAATGACAATAAAGAAAGAGAAGTTATCCATCTTATATCACGTGAAATCACCAGATTTCACTGCATCTATTGACCGATTTTTCTACACTTACTTGATATTAAGTTACCAACCCAATTTTTATCACATGGCTGGATAGTTGATGGTGAAGGGAGAAAGATGTCAAAATCTTTAAACAACGTTATCTCTCCAGAACAATTAATTGATCAATTTGGTGTTGATGGTACAAGATATTGTTTATTAAAAGAGATGCGTTTAGATAAAGATAATCGTTGTAGTGTTAGCATCTTAAAAGAGATTTATAATGCTGATCTTGCCAATAGTTTTGGAAACCATGTTTCACGTACTTTTGGCATGATTAAAAAGTATCTAAACGGCAAATTAGAATACCAAATTATTACTGATAATGCACTTCAAAAAATAATGATTTTAATAGATGAATCAATCGTTCAATTTGATCATTACTTTAACAGTTATGAATTTTATAGAGCGATTAATCTACTTTTAAAAATTGTTTTTGAATTAAGTAAATTAATTGATGATTTCAAACCATGAGAATTGTTTAAAAATCAGGAATTCTCACTTTTAAAACAACTACTTTTTACTTGTGTTAGGTGTGTGCAGGTATGCTATGTGTTGTTAACACCTATCTTAGTAAATACTGCTTCAAAAGTTTTTCATTTATTTAATTTCGCTGATGATGCCTGTAGAAAAGATCAATTAAGAGATGCAACTTTATTAAAAAAAATTATTATCTCTAATTCAATGGAAGTTTTATTTAAAAGAGTAGATTAAGAAAAGTAGTATTAGAATTTTATTGATTTATGCAATTAGAGTACCTCAATCTTATTTCTCAAGCTAAAGTTATTGCAGAAAAACAATTTAAAGCTAACCCTTTTTCTTTTGAAACAATTTGAAAAGAAGTAGTTAAACATTTCAAGATTTCAAAACAAGATGAACCAAGCTTAATTGGTCGTTTTTATCAAGATTTTCTTGAGGATCCTAACTTTGTCTATTTAGGTGATAGAAAATGAAAACTTCGTGATTTTATGAAGTTTGATGAATGGAACAAGATATCACAATCTATGTTTGTTACAAAGGAGATTTTTGAAGAAGGTTATGAAGATCTTTCCAATAAAAAAGTAGAACCTGAGGAAGGAGTTGGTGATTTCATTATGGGAAATGATGGTGATGACAATGAAACTGGCAGTGAAATAGTACAAGGTTTAATTAATGATTCATTCAGTGAGGAAAATCAATAGTAGATATGCTTGTTAACTTTAAATTGATGCTTCAAAAAGCAAAGCTAGGTAAATATGCAATCCCTCACATTAACATCAATAACTATGAATGGGCCAAAGCTGTTTTAACAGCAGCAAATCAAGCTAATAGCCCAATTATTGTTTCAGTATCTGAAGGTGCTTTAAAGTACATGTCTGGTTATAGTGTTGTTATCCCGCTTGTTAAGGGTTTAATTGAATCACTAAGTGTTAAAGTACCAGTGACATTACATTTAGATCATGGTAGTTATGATGCATGTATCCAAGCATTACAGGCTGGATTTAGTTCAGTAATGTTTGATGGTTCACATTTACCATTTGAAGAAAATTTCAATAAATCTAAAAAGTTAATAGAGATAGCACAAAAAACAAATGCTTCTGTTGAACTTGAAGTTGGTACTATTGGTGGAGAAGAAGATGGTGTTATAGGACAAGGTGAGTTAGCTAATGTTGATGAATGTAAACAAATCGCTAGTTTAAAACCAGATGCTTTAGCAGCAGGAATTGGTAATATCCATGGTATCTATCCTAAGAATTGAAAAGGATTAAACTTTCCTTTGATTGAAACAATATCAAAAATTACTAACTTACCCTTAGTTTTACATGGTGGCTCTGGAATCTTAGAAAATGATGTTAAAAAAGCAATTAGTTTAGGGATTTGCAAACTAAATATTAATACTGAGTGTCAATTAGCATTTGCACATGAAATTAGAAAATACATTGAATCAAATAAAGACTTGGATCTTAACAAAAAAGGTTATGATCCTAGAAAACTTTTAAAAGAACCTACTCAAGCAATTGTTGATACTTGCTTGGAAAAGATTGATTTGTGTGGTTCTAGAAATAAAGCATAGATGTTAAGTGCAGGGATAGTTGGTTTACCTAATGTTGGTAAGTCAACTTTATTTAGTGCTATTACTAATTTGCAAGTTGAAATAGCAAACTATCCTTTTGCAACTATAGAACCTAATACTGGCATTGTTAATGTTAGTGATGAGAGATTAGATAAATTAGCTAGCTTAATTAATCCTGAAAAGATAGTTTATACAACCTTTCGTTTTGTTGATATAGCAGGTCTTGTTAAAGGCGCCAGTCAAGGTCAAGGATTGGGTAATCAATTCTTAGCAAACATCCGTGAAGTGGATTTAATTTGTCATGTTGTAAGATGTTTTCAAGATAAAAAAATTGTTCATGTTAACAATACAATAGATCCTGTTTTTGATTTTGAAATTATTGTTAATGAACTAATCCAAGCTGATTTTGAATTAATAACAAACAGAATCGGCAAGCTTAAAAGAAAAGCTGAATCAGGTGATAAAATCGCTAAAGAAGAGTTTGTATTACTTGAAATTGTTTTAAATGGATTAAAACAAGGTCAAATGCCCATTCAGACTCTAAGTGAAAGTGAATTGAAAACAATTAAATCACTTAATCTATTAACAGCTAAACCTATTCTAATAGTAGCCAATGTATCTGAGAATGACTTATTAAACCTTGATAATAATGAAGCTTTAAAAAAATTGAATGCTTTTCTTGATCAAAAAAAGATTCCCAAGGCAATCACAGTTTGTTCTTTAATTGAAAAAGAATTAAGCGGTTTGAAATTAGAACAACGTCAATACTTTTTGGATGAACTTGGCTTAAAAAATTATTCAGGTTTAAACCGAGTAATTCAAGCTGCATATCAAACTTTAAACCTTTGGTCTTTTTTTACTTTTGGTAAAAAAGAAGTTAGAGCATGAACATTTAAAAAGGGTTGAAATGCTCCTCAGTGTGCTGGGCAAATTCATTCTGATTTTGAAAAAGGATTTATTAAAGTTGAAGTAATTAGTTGAGATCAATTGTTTGCAATGAAATCTTTACAAGAAGCTAAAAAACAAGGTTTGATAAGATTGGAAGGCAAAAATTACTTAATAAAAGATGGTGATGTTTGTAACTTTAAATTTAACGTCACTTAAAAATTAATCTATTTTCAAAATGAGTTTTTTCAACATTTATTAATTAAAAATTAATGCTTTTTAAAGTTGAATCTTAATTCATTTTTCAATACTTTTTTAGGCTTCCTATTCTTTAATTAAATTAATACGCTTTTCTAAAAGTAAAAGTTAAGAAAAGTACTTTTATTCAAACAAAAACGTTTTTATAATTTTAGAGATAGTTGTGCCAAGTAAGTATTTATTCACCGTTATAATTCCAACATACAATTGTTGTCAATACATAAAAAAGGCATTAGATTCATTGCTTTTACAGAATGAATATTTTTTAAAAACACAAGTACTAATAGTTAACGATGGTTCACTAGACAATACTAAGGAAGTTGTTAGTGACTATTTAATAAAGTACTCCAATATTAGTTATTTTGAAAAAACTAATGGTAATTGGGGTAGTGTTATTAACTATGTTAAAAAAAATAAATTAGCCTTAGGTCAATATATTACTGTTTTAGATAGCGATGATTATTTTTTAAAAGATAGTTTTAAAAAAGTGGCTCGTTTCTTTGGCCATGACATGATCATTGGCGCTTTTTATTGTTATATTAATGAAAATAAAACTCGTTTTTTAAAACCTTATTTCGGTAAGACTGGTGTTATTAAAGAACATACCAAATTAAGAACCCCCCATTCCCAACCTATTGCTAAATTTTACAGTAATAAACTTTTCTATGAACTACATGATCTTAAGGAAAAGTTATTCTTCCAGGATTGTTTAATGTATCACGATGCTATTAACAGAGTTGAAAGTGTTTTTTATTTGCGAGAGCCTTTAGCAGTTTGGTTTTCTACAAGACCTGGCAATTCTACAACAACTTCTTGAGAAAATCCAAATAAATTTAATGCTTGGTGTGAAATTCTCCAAAAAATGAATTTGTATGGAGCTGGGATAGTAATCTACATCTATACTATGCTACCTGGATTTCTAAAACAACTAAAGAAAAAACAACTAATACTGAATTTGAACCATAAACCAGCTTACACTTGATTACCTAAACCTTTAGCGTTTATTTTTGGTGGTTTAATGGCATTTAAAACCAGAAAATACATTAAATATCCTAAGTAATTTATGGCAGAAATGATAGAAGCAAAAAATCTTCGTAATGGGCAAACCATCTTCGGTCCTAACAAAGAGATTTTATTAGTACTGGAAAATACATTTAACAAAACCGCAATGCGCCAGGGAATTGTTAAAACTAAAGTTAAAAACTTAAGAACTGGGGCTATTGTTTGGCTTGAATTTACTGGTGACAAATTAGAACAAGTAATTATTGATAAGAAAAAAATGAATTTCTTATACAAAGATGGTAATAACTTTGTTTTTATGGATCAAAAAGACTACAGTCAGATTGAGATTAATGAAAAAAAATTAGAGTGGGAAAAAAATTTCATTACTGAAGAAATTGAAGTTACTGTTATTACTTATCAAGATGAAATTCTAGGAGTTAATTTACCTGATTTAGTTCCTATTGAAGTTGAGTTTGCTGAAGATGCTATTCAGGGCAATACTGCTAACATGGCAAGAAAAAAAGCACGCCTTGTAACTGGTTATGAACTTGATGTACCCCAATTTATTAATACTGGTGATAAGATTGTAATTGCCACTGTTGATGGCAATTACCGTGAAAGGTTTAACAAATAAATTAACTAGAACCCAAAGACGAATTGCTGTAGTGGAATTTATCTTTTCTCTCCTCTTTTTCTTACCTAAAGAAGCTGAAGTTATTCAAGCAGATTTTTTAGAGTATGATACTAAAGAACGACAACTAAATGAATGACAAAAACTAATTGTTAAAGCATTTAGTGAAAATATCTTCTCTTTTCAAAAGAAAATTGAAGAACAACAATTGAAAAATCAATTAGAAATTCAAACTAAATACAATAAAATATCAGGAAAAAAGATTGATCTTTTAACTACTGCAGTTGTTTTATGTGCACTTAGTGAACAAAAGGCACATAATACTGATAAACCACTTTTAATTAGTGAGGCATTGTTGATTATGGATCATTATTCACAAGGTGCTGAAAAAAAACAAACTCATGCTTTATTAGATAAGCTCTTGTAATGAAACGTAACTGAAGACAACACTATAATGTTTTTTTAGCTAATCTAGTTTTAGTATTTGGCTTTGCTTTAAATATTTTGGTTGCAAAACAATCTTTAAATAACACAACGCCCCAGTTCAGGTTTTTGTTTGTAACTCCTTTTCTTGGCGTTGTCATAGGTGCTGTTCTCTATTTTTTTGATGTTAAGTGGTTTTTAATTGACTATCCATACAAGAAATTTCACTTTCAAAAAAAATGAGCAATTGTTTATTTATCAGGGGTTATTGTATTTTTTTTAAATGTTTTAATTGGAGTAGTTTTACTTGTTGTAATGGTTAATTACATTACTAACCAAATTCTTGAAAGGGAATATGAAAGATTATTTACAAATTCATTACCTTATTTATGATCAACAACAGGAACTAGTATTGTTTTAAGTCTTATTAGTATAGGAATGAGTAAAACTGCACATTTTTTTATTGATATAGAGATTTTGAAAGCCAAAAAAGGAGAACCCACTGATCCTAATAAAACTGATAATAGAGCAGTTGTTATTAATCTTGATGAGAATAAAAAGAATGAAAAAGAACAGTCCCCTCCTTCTGCAGAAATGACTAGTCTTTAATTTCACTACCAAATAACCTTTTATGAACAAGCTCATTTAAAGTGTTATCTTTTAAAAAAGTGCACACAATAACTGTTGCAAATTCCAAAGCACAACCAGCACTTCTTGCTGTTATTAATTTATTTGCTACTGTTACTTTTGCTGTTGATCTGTTTTCAGACATAACTAAATTTGGGTTTGGAAAAGAAGAATAGATCTCATCTTTAGTGATAATACCATGTTTAAATAGAACATTCGGTGTGTCACAAATAGCAAAAAGATAAAGGTTATTTACTTTGAAATAATTAATTGTTTTAAGCAGTTTTTCATCTTTATCTAAGTGTTTTGTAGCTCCTATTCCACCGGGAATATAAACTGCATCAAATTCTGATAAGTTAATGGTGTTGACAATATTGTTAACTTCAACAACACCATTACTAGCAGTTATCTTTTTTAATGAATGGTGATAACATGTTGTTTGTATTGTTTTAATGAAACTAAATACAACCATTACATTAGTATATTCAACATCATTCATCTCTGGATAGACAATAACAAGTATCTTTTTCATTTTATTTAGTACCAAATAAACGGTCCCCAGCATCACCAAGACCAGGGATTATGTATCTATTGTCATTTAACTTTTCATCAATTGCTGCAAGAAATATATCAACATGAGGATGCATTTTTTCTACTTTATTAATTCCTTCAGGTGCTGCTACTATAGCAATAACACTAATTTTGATAGGTTTATCTTCTTTAATAGATTTAATAGCAGTTAACAATGTAGTTCCAGTAGCAAGCATAGGATCAAGAATAATAACATGTGAATCAGAGATGTTTTCAGGCATCTTTTTATAGTATGAAATTACACTGGTTGTTTGGGTTTGACGATAGATTCCTAAATGACCAACTCTGATTTTATCTGAATAGCGAACAATAGCATCAATCATTCCAAGTCCAGCACGCATAATAGGTACAAGAACAATGTCATTTTTTAATTTGTAGCCCTTTGTTTTAGCAAAGGGAGTTTCAACTTCAACTGTTGCTAGTGGTAGCTGTTTAGTTGCTTCAAAAAAGAGTAATGAAGTGATTTGATTCAAGGCCATGCGAAACTGGGAGGTTGTTGTATTTTTATCACGCAGTTTTGTCAATTCATTCAAGATTAAAGCATGTTGAACTTTTTTTATCACGTTAGTTATCTTTAAATAAGTTTAAACTAAAAATTAGTTGAAAAGTTTATGATCATCTTGATGTTCAAATTCATCAAACAGTTGTAATTGTTCCATCGTTTCAACGTGTTTTTTAGTAATTTTGGTTCTTTTTTTGAAATCATTTAAGGAAGCAAATGGTTTTTCATTTCTTGCTTCTACAATTGAAGAAGCTACCGCTTCTCCCATTCCTGGAATAGTGATGAAAGGCGCAATTAAAACACCATTATGTTCAACAAACATCCTTGCATTAGAGTTTTGAATAGAGATTTGTTGCAACTTTATATTGCGTGCCATCATTTCTAAATAAACTTCATAACTTGTTAAAAGCTCCGCTTCTTTTGGTTTAATCTTTTTTATTCTGTAAAGCTTATTAAGTTCATCTAAACGGTTTTTAATAAATTCATACCCTTTTTCAAAACCATTAATATCATGTTCCTTTAGTTTGAAACTTAATAAACAAGCATAGTAACTCAAAGGGTGATAAAGCTTAAATCAAGCAATTCTTCAAGCCATTAAAACATAAGCTGCAGCATGTGCCTTAGGGAATAGATAATTAATTTTTAAACAAGCATTAATCCAATATTGTTCAACACCACAGTTTTGCATCAGGCTAACTTCTTTAGCATTTACTTTAATACCTTTTCTTACTTTTTCCATGATTTCAAAAGCATCTTTTGCTTGCATCCCTTTATTAATTAGATAAAGCATGATGTCATCTCTACAAGCAATTACATCTCTTAAAGTTAACCTATTGCTTTTAATTAGTTTTTGTGCATTATCTGCTCACACGTTTTTACCATGGCTTAATCCTGAAACTCTAATTAAGTCAGCAAAGTCTTTAGGTTTTGTCTGTTCTAAAATTTTTCTAACAAATTTAGTCCCAAATTCAGGGATACCAACCGCTCCTGTAACTTCATCAACAATGCCTGGTTTTAGGTTTAATGGTTTGTTTGAAGAAAACATCGAAATTAAGTTCTTATCAAAGTGAGGGATATTTTGGGGATTTATCTTAGTTAGATCAGCTAAGTGCTTTAACATTGTTGGATCATCTTGACCTAAGATATCAAGTTTTAATATGGCATCACCTAATGCATCATATTCAAAATGGGTGGTCTTTCATTCACTTTCAACATCATCTGCTGGAAAACCACAGGGAGTAAATTCATAAACAGAATGATCACTAGGAAAGATCATAATTCCCCCTGGATGTTGCCCTGTTGTTCTCTTAATTCCTATAAGCTTTTGTTTAAATCTTTCAATTTCAGCAGTAGTTGCAAGATCAACACGTTTAATGATTTCAAAATAGTTTCTAGCATAACCATAAGCAGTTTTTTCAGCAACTGTTGCAATTGTTCCTGCCCTGAAGGTGTTATTAACTCCAAATAATTTTCTGACATAATCATGGGCTTTAGCTTGATATTCGCTAGAAAAATTAAGATCGATATCAGGAATTTTATCACCAGAAAAACCCATAAAAGTAGCAAAAGGAATGTTATGACCATCACCTTTGAAACTTGCTTTTTCATGGCATTTAGGACAATCACGAATCATCAAATCATATCCATCATCAACACTGTCACTAACTTCAAAGTAATGACATTGTTCACAAAGATAATGAGCAGCTAATGGATTGATTTCTGATATACCAATTAAATTAGCAATTAAAGAAGAGCCTATAGAACCACGTGGACCTACAAAATAACCATCTTTAACAGATTGTTCTACTAATAGATGGGAAATTCAAAAGACAATTCCAAAACCATTACTAATGATTGCATTTAGTTCTTTTTCAATTCTTTCCTTAATAAGTTTAGGTAAATTTTTACCATACCTTTTTTCAGCTTGTTTTCAAGTTTTATCAATTAATTTTTGGTTAGAATCTTGCATCACTGGTGGATAAAGCTTGTTTTTAGTTGGTACTAATTCATTTAAGTCAAGTAGCTTCACTATCTTATTAGTGTTTTCAACGACTAATTTATAGGCAATATCTTCTCCTAAAAAACTCATTCTTTTTAGCATCTCATCAGTGGTGTGAAGAAATACTTCAGGAACGGTTTGTTCTTTTTCTTTATTGTTAAAGTGTCTATGTCATTTCCCACCTAAGCCCTTAGCACAAACTATTGCCTTATAATATTCATTTTCCCAAGGATGGATAAAGTAAGCATCAGATGCAACAGCAACTAATTTCTTAAGTTTTGTTGCTGTTTTGATAACTAGTTTGATTGCATCATTAATAAGTTCTTTTTTCAGTCCTTCACGTAGTGTATAACCAAGGTAAGCATTGGGTTGTGAAATAAGCACAAAGTCAACTTTTTCAATTGCTTTTTCAAGTTCATTAATTGGTTTTGTTAATGCAGCTTTAAAGATGTCACCCTGAACAGGGTTTTCTGTTAATAAAAAAGATTTTCTAAATTTAGCTAAGCTGCTAGCTAAAACTAATGGTCTGTTAGCATTATGATCTGTTAATGCAATTGATAACATCTCATATAGGTTTTGAAAGCCCCGTTGGTTTTTTACATAAACGATTGCTGTGTTTGTAAATACCCTTTTCATCAGATCAATTTGACACTTTTTATTTAGGTTTTGATCTATCTCTGTTAAGGTATTAATACCCATTTCTTTTAACTGCTTTTTAAAGTAAAAGAAAACTTTTTTTAAAGCTTCTGTATCATATTCAGCACGGTGTAATCTTTCATCATCAAATTCAAGTTTTAGTTTAGAACAAATATTGCTTAAAGTATGGGATGAAAACAAGGGATTCAATGCTCATGATAAACATAAGGTATCAATCAGTGGGTTTGTTAATGGTTTGATGTTGTATTTTTCAAATTGAGTTTGCAAAAAGGGTAAATCAAAATTAATACCATTATGAGCTACCATAACACAATCATCTAGATAATTTCTTATCTTTTCTAAACCTTGCTGTTGATCAATACCGCCTTCAAGCATCTCATCAGTTATTTTGGTGATTTCAGTGATTGTTTTTGGGATAGGTTTGTCAATTTTTAAAAAGAATTGCTGATGATCTATCTCGCTGTTATTCTTAATTTTGCGTGCTGAAAACTCAATAACATCATCATACCTACCATGTAATCCAGTGGTTTCAATATCAAAAATAACAAAGGTGGCATCACTTAACTTAGTGTTATCTGGGTTGTGAACAATTTTGATGTGATCATCAGTTAAGTTGAATTCCAAACCATAGATAGCTTTTAAATCATATTTTTTAGCCACTTCATAAAACTTGGGATAAATATGGATATTATCTTTATCTGTAACAGTTATTGCTTTTCAACCTCTTTCTTTAGCAAACTGTGCATATTCTTCAATGTCATTGATACCATCAAAAGCAGTCATTTTAGTATGAAAAACTAACTCCACTCGCTTTTGTTTTGATAAATCTAACCTTTTATAGTTATTAGGTATTTCAACAGGATTTATCTCTCTAACAATTCCATATAAGATTTGAGTATTAGGGTCACGTTCTACTTGGATATGGGCTTTTATCCAATTGCCAATAGTAATTCCTTCAATCTTTTTTTCATCAGTATAGGATCATTTCAAAATTAACGATCCACCTAATTGAAAATCAGTTACATAGATATTAAGTGTTTTTTTACCAGTTAAACTTTCATGGGTTTTTAATTCAAAGATTTGCCCAATAATCTTGACATCATCGATCTGTTGGTTAATCTTGTGGATAGGGATAAATTCTGTTGCAAATACCTTTGTTTCAAACTGCTTATCATATTGAGAAACAGTTACAGCTTTTACTTTTAATTTAGATTCATTTTTATCAATAGGATAAAGTGCAATGAAGTTAAAGTTTTTAAAACCAGCATTATTCATCCAAAAAATGAAAGATTTACTTTTTTGAACTAATCATTCAGTTAATTCTTTGGTTTGACATTGTGCTTTTAATTCGTCATTTTCATAACTAAGAAAATTGGGATTACTTAACTCTTGTTCAATGAGAATTTTGTATTTCTTATCTTTTGAAAAGAAACTGTGAAAATAGTCTTTAATAATAGCTATCGTTACAGAAGAAAAACTATTTTTTGATTTAAAGAATGGCTCATTCTCCTTAAAGGTAATGGTAAGTTTAAAAGATTCATTATCAGCTTTAAATCCCTCATTTAATTCATTAAATCCTTCATATAAAGCATTTCAAATATCAATAGTTAAAGGGCGACTAATAGTTATTGCAAATAGAAAAACCTTCTCTTCAATATATTTATTAAAGTAAATTGATTCAATCCTTTCAATTAAGCTGTTAAGCTCATTGTGGTCAAGGATATTGTTATCTATCAAAAAATTGGAGAGTGCAAGAACCTTTTTATCTTTTTCATTCTCAAGATTAAATACCATAAGCGTTTATTAAAAAAATCAAAATAAAAGATCGAAATTTATCAATAATTATTTTTAGTTTTAGCCACTTTTTCGTAAAAAAAAAAAAAAAAAGCAGTTTTTGCTATTTTAGACTAAAAGTGGTTATATACCAAAATTTAAACCTTCTTTTGGTTAGTTCTTACTAACAGTTTTAAATGTTACACAAGTTTTTCAAAGTGTGAATTTAAAAACTTCAAATAGTTGTTTATTCTGTATCTAATTAACCTTTAATCTTTCAATAATGAAAACAATTCATAAACTATTTTTAGGTCTTTGTTTACCCGCAACATTAGGTCCTTTACTTGGAATTGTTGTTACAAATACTGACCAAAGTATTAAGTTTACAAGCAAATCAAATTCGATTAATAAGAATAACCAAAATAAAGAGTTGGCTCTACTTAGAGATAATTTGATGAACGAAGCGAAAGTTGATGAACCACTTTCCTTTGAAAAACGGTTTGAAAACTTTAAAAATAAGTATAGTGATATACATAGCTTAAATAACAGTGTTTTTTCACTTCATGACGTTTATGACTTATTAGGTGGATTTAAACAATCATTGACAACATTTTTTGATGAAGTGATTGCCCAACAACAAAAGATCAAGGATGCAGATAAGATCTTTCCAAGTACTAAAGATAATCCACCTAAAGAAGAAAATCCTAATGTTTTAGATACACTAGCTAACTACCAAGGAGCAGGATTTTTCCCTAGTTTAGGTAAAAATGGTTTTAATTTACCTGAAGCAGTGTTCCAAAATTTCACTGATTTTAGGATTAATGACTACAAGATTAAAAATTTTAATGTTGATCTTGTTAGTGAAAATGACATTATTAAACATGATAAAGTTCGTTATGCTTTTGAAGTTAAGTTCAATATTGCTTTAGTTTTATCTATTAATAAGTCAAATGTTGATTTTGACTTTGATTTCATTTTAAAGACTGATAATTTCTCAGACATTGAAAACTTTAATGAAATTTTCAACAGAAAACCTGCTTTACAATTTCGTTTTTATACCAAGATCAATGTACATAAGTTAAGTTTCAATGGTAGTGATTCCACTTACATTGCCAATATCTTGTTACAAGATCAGTTCAACCTATTAGAAATTGATTTAAATAAATCTATTTATGCATTAGATCTTGAAAATGCTAAAGAACGCTTTGATAAGGAATTTGTTCAACCTCTTTATCAAAAACGACGTGAAGCAAAGCTTGCTTGAGAAGAAGAACAGAGACGCATTGCTGAAGAACAACGTAGACAAGAAGAGGAGAGAGCTAGAATCTTAAAAGAGTTAAAAGAAAAAGCTGAGAAAGATAAAAGAGTTAAAGAAGCACAAAACAACCTTCAAAAAGCACTTGGTAACTTAGATACTTTCTTTAACTTCTTTAGCAGTGGTCAAGATAGAGTTTTACTTGGTTTTGATCCAAATAAATACAATGTGCAAACTCGTGAAGGTTTGTTTAAAGCATTACAAATTTCCTATTCTAACTTCAAAACTTGAACATTCTATATCTCCTTGTTGGGGTGAAAAGAAGGTAGTGTTAAACTGTTGAAAAAACCTATCTGAAATGCCTTGAGAGATGATAAAGCATTTCAATATGCTTTTGGTTTAGGTCCAAATACTTCTGAACAACAACTTGGTAGAGTAACCCTACCTGGTTATGGTTATGAAGGAATTAGAATGAGTGATTGGTTGAGGTGAGCATTGGGTTACTATACTAGTTTCACTTTAAGTCCACCTAAAAATGTTGAAGCTAATCTTATAGGTGATGCTAATGATAAAAAACACATTTGAATCTCACCTCATACTTTCAAATTAAACAGAGAGTATGGTGATGGTGAAAGATTCAAAGGTAAAGCATATCGTTTTAAACTATCAATAAGTTTTGAACTAGAAGGTCATTTAACTGCCCACTGATGAACAATTGCCTTTAGAGGTAGTATTCCTGGAAGCTGAAGTGGTAAGTTAAGAGTTACCCATGAGTTTGATGGTGATGTGCCTTACTATAGATTACATACAACTCCACCACAATATCGTTTAACTGATGATATGAAATTATTGTTTGTTCCGCACAGTATCCAAAGGGTAACTGCAGTTGGTAATGAAAGCATTAATGGTCTTCTCAGATCACAAAACCTTCATAACTTGGAACGTCAATCATATGAAGCGACTGCTCCTATTGATTTAATATCATATATGCTTTATGCAATTAGTGATAAAAAACCACCTCAAAAATAAGTTTTATATCATTAACGTTTAAATAAATATTATTGCTAAGATTACTTTAAACTTGTTAGCAAACTTTAATCATTTTTTAGCTAAATTAAACAACCAGTTAATTAGTAAACAGTTCCAGAATTAAAATCAAATATTAGTTAGTTTTTATTTTGTATTTTCAAAATTCTACTCAATTAGGATGATGGTTTCTTGCTGAATTGATAGGAACATTTATCCTAATTATCTTTGGTAATGGTGCAGTTGCCCAAGTTAATTTAAAGAAGATGGCTACAAGTGAAACAAAAGCCAAGTTTTTAACAGTTGCACTTACTTGAGGAATAGGTGTTTTATTTGGTGTTTTAACTGCTAATGCTATCTTTAAGGGTAGTGGTCATTTAAACCCTGCTATATCATTATTTTATGCAATTAATGGCAGTATCAAATCACCTACTGCATTAATATGACCTGGTTTTGTAATTGGGATTTTAGCTCAATTCTTAGGTGCAATGATAGCTCAAACAACACTTAACTTTTTATTTTGAAAACAACTATCATCAACCGATCCACAAACAGTTCTAGCAATGCATTGTACAAGTCCTAGTGTATTTAACATTACTAGGAATTTTCTAACTGAATTTATTGCAACTTTAATATTGATAGGTGGAGTTGTTGCTGCTAGTCACTTTCTTCATAACAACCCAAACTCTGTTCCTCCTGGATTTATGGGGCTTTGATTGGTTGCTGGGATTATTATTGCTTTTGGTGGCGCTACAGGCTCCGCAATTAATCCTGCAAGGGATTTGGGAACTAGAATTGTGTTTCAATTAACTCCAATTAAAAATAAGGATGCGAATTGAAAGTACAGCTGAATTCCAGTAATTGCTCCTTTATCTGCAGGATTAGTTTTATCAATAATTATTGGGTTTTCCCCTGCACCTGTTCTTTAAAGTTTTTGGTTTTAACAGTAACGTGCTTTTTAGTTAAAAATGAGTGGTGCAGTTTGCATCTAGCTTCATAAGCTTCTGCATCACCAATTAAGACAAGGTTATCATTAGTATTTTTATTTACTAGTCTTTGGGTTCGTTGTGCTAAACTACCACAAACATTGCAAATAGCATCAAGTTTATTAACTACATCAGCTATTGCTAAAAGTTGGGGAATACAACCAAAAGGCTCAGCTCTAAAATCAGTATCAAGCCCAGAAATAATTACATTTGTTCCTATCTCATTGAGAGTTGTAACAACTTCTATAATCTCATTTGAAAAGAATTGTGCTTCATCAATGGCAACAATTTGATAGTTTTTATCAACTAAGTGATCATAGATCTCAAAAGGAGAATTAATAGTTATTGCTTGATCATATTCACCGTTGCGTGATTTGACAATATTAGTTTGTCTAGTATCAATTATTGGTTTGAAGATAACAACTGAAATTTTGGCTATCTTTCACCGTTTTATTTTGTGAAGTAATTTCTCTGTTTTTCCAGAAAACATTGGGCCACAAATAACTTCAGTTCAGCCCTTTTTGGTTTGAAAAGATGGTTGATATTTACCCATATAATTTCGAAATTATAATTAATGACACATGAACTTCTTGCAAAAACCAAGGGGAGTTAAAGATTGGTTTGGTGATGAATTAGTTTATTTTAATTGGATTGTTAAAAAAATAAGATCTTTAGCATTTAATTGGGGTTTTAGTGAAGTTAAAACTCCGTTGTTTGAAAATGCACAACTTTTTCAAAGATCTAATGCTAATGCTGATATTGTTCAAAAAGAACTATACCAGTTTTTTGATAAATCTCAAAGAGAATTAGCTTTAAGACCTGAAGCTACTACACCAATAGTAAGACTTGCTTGTGAAAACAAATTAATGCAAGAAGCAAATTTTCCCTTAAAGTTATTTTGCATTGGTTCAATGTATCGTTATGAACGTCCACAAAACAATAGGTTTCGTGAACATTGGCAATTTAGTTGCGAAGTATTTGGTTTTTCCAACCTGTTTATCTTTTTAGATACACTTTTGTTTGCTAACTCTTTGCTTGAAGCACTTGGAATTACTGGATATGTGCTTAAAATTAATAATCTTGCTAACTTTGAAACACTTAGTAAGTGAAATAAAGCCCTAAAAGATTATTTAACTCCATATAAATTAGAACTAACTGAGCTTTCTCAAAAAAGATTAGAAAAAAATCCTTTGAGAATTTTAGATGACAAGATAGATCAAAAAAAATCATTTGTTAAAAATGCTCCTAAAATTACTGATTTTTTAGATGCAAGTGCAAAACAAGATTCAGAATTGTTAAAAACACAACTAAAAAAACACAATATTAGTTTTGAATGAACAGACAATCTAGTTAGAGGATTGGATTACTATACTGGATTTGTGTTTGAATATGTAAAAAATCAAGACACAATTTTAGCAGGTGGAGTTTATGATAACTTAGTTGAAGAATTAAGTAGTAATCCAACTCCCGCATTAGGTTTTGCTTGTGGAATTGAACGGTTAATTAACTGTTTAGAAATTGATAAAAAAGCATTTATTTTGAATACTAAACCAAAGCAGATGTTAGTAATTTGCTTATTTGAAGAAGCGCTTGAAGAATTGGTTTGACTAGCTAAATTATGAAGGGAATATAACCAAGTAACTATTTATCCTAAGGTTATTAAAGTTGATAATGGGATTAGATTAGCAAATCGCTTGGGTTATACTTTCATTGGCATTGTTGGAAAAACTGATTTTGACAAAAAAGCTATTACAATCAAAAACTTAGTATCTAAACAACAGACCATTTACACTTGAAATGAACTTGGAGAACGAAATGTGTTTTAACCAACGAATTTTAATTGGCTCAATTTCAACTGAACAACTCAATAAAACAATAGTTATTATTGGGTGAATTAAACGGATTAAAAAGTTAGGTGAAATTAACTTTATTATCGTTGGTGATAAATCAGGAACTATCCAAGTAACTTGCAAAGATAAAGAACAGATTCAACAACTTACAAGAGAAGACATAGTTATTGTTAAAGCCAAATTACAACGCTTAGATAGTGTTAGATTTGAACTGATAAATCCAACTATTAAACTTTTTTCAAAGTCAAAAACTCCTCCTTTAATTATTGAAGATGAAACTGATGCTTTAGAAGAAGTTAGGTTAAAATACCGTTACCTTGATCTGAGAAGACGTTTGATGCAAAAACGATTGTTATTGCGTCATCAATTTATATTAGCAATTCGTAACTGATTTAACCAGCAGGGTTTTATTGAAATAGAAACACCTACCTTATCCAAATCAACTCCTGAGGGAGCACAAGACTTTTTAGTTCCTGCAAGAATTAGAAAAGATTGTTTTTATGCTTTAGTTCAAAGTCCACAAATCTATAAGCAGCTCTTAATGATTGCAGGAGTTGAAAAATATTTTCAAATTGCAAGGGTCTATCGTGATGAAGATAGCAGAAAAGATCGTCAACCAGAACACACACAAATTGATTTCGAGATCTCTTTTTGTAACCAAAAAATGATTATGAATCTAGTTGAAAAACTCTTTTTTAGTGTTTTCTTAGATGTTTTTCAAATCAAAATAAAAAAGACTTTTCCTGTTTTTAAATTTTCAGAACTTTTTGAAAGATTTGGTAGCGATAAACCAGATTTACGTTATGGTTTTGAAATAAAAGATTTCACCTCGCTTTTTCAAGATCATCAGAATCAGTTCACTAAATTAATTGAAGCAAAAGGCATTATTGGTGGTATTGAACTTACTAATATTGAGTTAAGTACAGACAAAATTAAAGCATTAAGAAAAATTGCTAAGGACCATGATGTGAGTTTAGAAGTTCATAATAAAAATAATTCAACATTAAAAACTTCAATTAAATGTGATGAAAAAAACACTCTTCTGTTAGTAGCAAATAAATCTAAAAAGAAGGCATGAACTGCTTTAGGAGCAATTAGAAATGAGTTGAAATACCACTTGGATATTGTCAAACCTAACCAATACAGCTTTTGTTGAGTTGTTGATTTCCCTCTCTATGATTTTGATGAGAAAACAAATCAGTGAATATCAAATCACAACATCTTTTCAAAACCTAAACAAGAATGAATTGATAATTTTGAATCAAATAAAAACGAAGCATTAAGCGAACAGTTTGATCTTGTTTTAAATGGTTTTGAAATTGGTAGTGGTTCAATAAGAATTAATGATCCAATTGTTCAAAAAAGACTAATGAATTCTTTGAACATTGACCCAAATAAGTTTGCTTTTCTTCTAGAAGCTTATCAATATGGTGCTCCTGTTCATGGTGGAATGGGACTAGGTATTGATCGTTTAATGATGATTCTTAATCAAACTGATAACATCAGAGAAGTAATCGCTTTTCCTAAGAATAATCATGGTATTGAAGTCCATACAAACGCTCCTGATAAAATTGACAAAGAGGAGGTTAAATGATGGATAAAAGAACTAGTGAAATAGAGTTTCACTTAAAGAATCTTTTGGCTTGTGATGCATATAAACTTTCACACCGTTTAATGTATCCACAAGATACACAAAACCTTTATAGTATGTTAACTGCAAGAGGTGTTTATGGTGATTTTAAGGAGTTTGTTTGAAACCATGATTTTGCTAAAGAGATCCTTTTGAATGTATTTAATGGTTTTGTAAACAGTGTAATTGAAGTTAAAAAAAACAAATTGCTAGCTGCAGCATTGACAGATAAATTAGTTAGTGTTTTTAATGATCATGAATTGGCTAATGAATTCACACAACACATCTGTCATTTAGCTAGTTTCTTAGAGAAAAATAAAAAAATGCCGTTAGTTGCAAAGATCCATGAAAGTGATCAATCATTACCATTTAGAACTCCTTTAATAACTATAGAAGGAGTTGAAAATATTCCAAACAACTTTGTATGGTTAGTTAATTACTTTGAAACTGTACTTCTAGAAAACATTTGGTTGTTTCAAACTGCTTCTACAGTTGCTAAAAGAATTAAATCTTTACTTGAAAAATATGCTAAAGAAACCGCAGATGAAACAAGTTTTATTAATTTTCAATGCCACGACTTTAGTATGCGGGGCATGAGTAGTTTGCAAAGTGCTTTGTATGTTGCTAGAGCACACTTGCAATACTTTACTGGAAGTGACACGATCTTAGGTGGGGATAATTCTCGTTCAATTTTAGCTTCTGAACATTCAGTGATGTGCGCAGATGGTAGTAAACATGAATTGAAAACTTTTCAACGTTTATTGGAAAAGTTTAAAGATAAAAAACTTTCTTTAGTGATTGATTCTTATGACATGTGAAATGTCCTTGATAACATTATTCCAAGGTTAAAAAACTTAATCTTAATGCGTGGTGCTACGCTTTATTTGCGTGCTGATTCTGGTAATTATCAAACTCTTATTTGCAATCCTAATTACAAAAAGCAAGATAAAAGTACATGAGCAATGATCGATTACTTAGATCATCATTTTAGTTCAACTATAAATAAAAAAGGTTATAAGGTTTTAAACAAGAAAATTGGCATTATTTATGGTGATGGAATCACCTATCAAAAGATAGAATGGATCTTAAATTGTTTAAAAAACCATGGTTATTGTTCTTCAAACATTATTTTTGGAGTTGGTAGTAGCACTTATCAAAATTTAAACCGTGATACTTTAGGTTTTGTATACAAATTGACTGCTATTAAAAGAAATAATAGATGGATAGGCGTTAAAAAAACTCCCATAACTGATCTATCTAAAAGTTCAAAAGGCGGTAGATATAAAACAAAGCGATTAATTACAGTTTATTAATCAATTTCTTTCCATTTTAAAGCACGTTCAACTGCTTTATGTCAACTGTTAATTTTGGTTTTTCTTATGTTTGGGTCCATAGTGCTTTTGAACTTTTTATCAAGAGTAGTGAGTTTTTCAAGTTGATGAATGTCTTTTCAAAATCCACAAGCAAGTCCAGCTAAAAAACAAACACCAACTGCAGTGGTTTCTTTATTTTTAGGGATAGAAACAATTACATCTGCAATATCAGCTTGAAACTGCATTAAATAGTTTGATTTAACAATCCCCCCATCAGCTTTAATGCTAGTAATCTTATAGCCTAGATCACTTGCCATTGCATTTAATAAATCATTAGTTTGAAAAGCAATTGACTCTAAGCTAGCTTTTACTATGTGCTCTCTTTTAGTGCTTGCTTCAATTCCTAAGATAATACCCCTAGCACTAGCATCTCATCAAGGAGCTCCAAGTCCACTGAAAGCTGGTACAAAAACTAGGTTTTGTTCATTTTCTTTTGCAAGTTCTGCATAAAAATCACTTTCCTTTTCTGAATAGATAATTTTTAATGCATCCCTTAACCATTTTATAGCCGCACCCGCTACAAACACACTACCTTCCAATGCATATACAGGTGGATGATTTTCTAGTTGCCATGCTACTGTTGTGAGCAGATTGTGCTTTGAGAGTGTTGGTTTATCACCAATGTTCATGAGTACAAAACATCCAGTACCATAGGTATTTTTTACCATTCCAGGTTCAGTACAGAGTTGACCAAACAAAGCTGCTTGCTGGTCTCCTAAAACTGCTCTAATTGGTACAATACCTTTAGCATTACTAGATCAGTGATTAGTTTCAATATCACCAAAGTAAGCATTGGAACTCAGAACTTTAGGTAAGATTGAAACTGGTACTTCAAATAAATCACATAACTCTTTGGATCACTCCATTTTGACAATGTCAAATAAAAGAGTTCTTGAAGCATTTGAAACATCTGTAACATGCATTTTTCCATTAGTTAGTTTTCAGATTAATCAGCTATCAATGGTGCCAAATAACAACTTTTTTTGCTCCATTAGTTTCTTTGCTAAAGGAACATTTTTTAAGATTCAAGCTATCTTAGTAGCACTAAAATAGGGGTTAATAGGTAATCCAGTTTTTTGTTTTACTTTGGTTTGGATTAACTTATCCTCATTGAATTTTTGACATAGTGCTGCAGTTCTTTGATCCTGTCAAACGATGGCATTATAAACAGGCAAACCATTTTCTTTATTTCATAAAACTATTGTTTCTCTTTGATTGGTAATACCAACTGCAATCACTTCATGAGATTTGATTTGTGCTTTATTTTTAGCACTTTGCATGGTAGCTAGTTGGGCTGATCAAATTTCTAGTGGATCTTGTTCAACTCAACCACTATTAGGAAAAAAAGTGTTAAATTCGTTTTGTGCTATTGCTATTTGGTTAAGATTGTGATCAAAAACAATTGATCGACAAGAACTAGTACCTTCATCTAAGGCAATAATGTATTGTTTTTTTAGATCCATGATAAGTTGTAATTAGGATTTCAATTTATCTTCTTTTTTAACTTTAAATTAAGGAGTTCAACTGCTCTCTTAGCAATAGCAGGACTTGATGTTAAACCAGGGGATTTCATGCCACCAAGGATGATAAAGTTAGGATTGCTTTTTGCTGTTCTAATAACAAAGTCATTTGTTTCAATATCAATAGCTCTTGAACCTGCAAAACTATAAATACTATTTTCAAATTGCAATGAAGGAACCATTTTTTTACCAATAGTTTTAATTTGGTTAATTGAATCTAAATCTATGGAACGAGTTTTGTTTTTTTCAATTCCCTCAACTGCATTAGGTCCCACAAGGATATTACCATCTAACATCTCAGCAACAACTACTCCCTTACCATGGATAGTAGGTACCATAAAAATAATTGTGTTAATCTTAAGGTTGTTTTGATTTTTTAAAACTAGATATTGTCCTTTTCTTGTTGTTTGTTTAAAATTATCAACTTGTGTAGTTTCTGCTAGTCAATCTGCATAATGACCAGCTGCATCAATTAACTTTTTGGTTTTAAATTGTGGTGTTGTTTCATTGTTAATAAAAACTAAAAAATCATCATCAGAATCTATTTCAATTTTTGTAACTTTTTTATTTGAGTAGATGGCAACATTGTTTTGTAAACTAGCAAGGGCTAAACACTTGGTTGCAATTAAAGGGTCAATTAGTCAACTACCTTCAACTTTTAAACTAGCTACTACATTTGGATTGATAAACGGTTCTTGCAATAAAGTTTGTTGTTGATCTAATATTTGAATGTTTTCAACAGGAATTGAGTTTTTAATTCCCCTTTCTTTTAACAAATTTAATTGAAGTTTTTCTTCATTGTTGAAAGCAACAATTAAAGTAGCAATTTTTTTCCTTGGGAAGATTAGTTTTTTAAATCAATCCTCAATTCAGATTTTTCTCCCTAAGATGTTGTATTTGGCAGTTAGCTTATTAGGATTAGGATCAATTCCTGAATGGATTACACCACTGTTAGCCTGCGAAGTTTCACAACCCAAAAAAGCATTTTTTTCTAAAAGTGCAACTTTTAATTTATATTGGCTGAGTTCATATGCACAACTAGTACCGATGACACCCCCACCCACAATTAAAACGTCAATTGTTTGCATTAATTAATCTTCTAGTATTTAGTTAGTTATTTTGCAAAAAAGGATTAGTTTTATAGTTGTGATTTACTTTTTGGTATCTCTATAAAAAGTAGTATCAAACATTTTTTTATCTTTTGATTTCTTTTTACTAAAAATAATTAGCTAAAATACCGTCATATATGGGAAAGCTATTATTTGGTAAGTTAGTTTTTAAAAAGAGCTTGTTTTTACTGAGTGGTATGAGCAGTTTAGCAGTTTTTTTAACTGCATGTGGGGCTACCAAAATATTTGATTCCTCTGTACAGCTATTAGTGTCAGATAACTTTTCCACACTTGCTGATAAATCATTTTCACAAATGTCCTATGAAGGAATCAGGAGCTTTTTTAAAAAAAGTAAGGGTGTTGATTTACCTGAAGCAGATAGTTCACAATTACAAGAAGGCAATGGCTTGTGAAAACGTCCTGGTTTTACATTAAGTGATAGAATTGCTACTTTTAATAACATCAAAAATGATGGCTCTGATGTTATTGTTGCAACAGGTTTTAACCAACAAGAATCACTTCAAGCAATTACTTCTGATGACATTAGGTTTCAAAGTGATAAAGAAAGCTTAGCTAAAACAGGTTTTATTTTTGTTGATGGAGCTATTGAAAAGGAGTTTAATAAAAGAAATGGTGTTCCTCAATTTAAGTCAACTCCTACCAATATCTCATCAGTTGCTTTTAGAAGTGATGATGGTTCTTTTTTAACTGGAGTTGCTACTGCAGTTTACTTAAATCTTAACCAAGAATATTTCCTTGATAAAAGTGGTTGGTCAACTAATAGTAGTAATAATAACGAACTAACTGTAAGTGGTTTTGTAGGTATTGCTCTACCTTCAACGCTTTCTTTTCTAAATGGTTTTCGACTAGGTATTGCTTATTTTAATGAAGTGATTTATAAACATTTAAGCGATGCACAAGATTCATCTGCACAAGTGACCACTTCTAAACAAACTGTTTTAAAACAACTTCAAGTTGCAAATGGTGAAAAAAGGATTAAAAAGATTAAATGGATTTCACCAAAACAAGGAAGTGATGGAGAAACTATAAACATTCAAGATCACCAATCAGGTTCTTTTTCAGATACTGAACCTAGAGCAATAACAATAGCTAATAATTTAATTGATAAAGGAGTTAATGCTATCATTCCTATTGCTGGACCACAAACGAATTTAGTGGTTACTCAAATTGCTAGAAGACAAGCCCATACTGCAGTTATTGGCGTTGATAGTGCACAGGAATTGCTAGATATTAATATTGATGCTCCAAATAAAGATAAGTTAAAAATGGGGAATAAAAAGATTATTCCCTTCTCTTCTATTAAGGCTTTGGATGTTGCTGTTGAAAGTATCTTATCAACATTAGAAAAAGGTTCCAGCCAAAATGGTTATCAAGGCTTTGGATATAACAACATAGGTACAGTGAAAAACAACTCTGTTGGGGTTAGTGAAGCAGGTTATGAATTTTTAATAGATCCTGTTTTTTGAAAAAATACTAGTTCAATGCAAGCTATGTCTTTATCAGCAAGTCTAAAAGCTAATGCAGCATCTTCATCAGATAATAAGAAAAAATTATCAGAAGTTGCTACTAAGAAAAATGAAAACGGTTCGACAAAAAATGGTAGTAATGACATCATTGACAAATATGCCAAACTCTTAACAAAATCTAGTTCTTCAACTAGTATGAGAAACGGTAGTTCAGATAGCAATCAACAGAATTTTAAAACAACAGATAATGATGGTGATTGAACTATTGTTGGTGATGAATTAGGTAAATATAAGTCTAGTGAACTGCCTATTTTTACAGGTAGTTCTTCATACCCAACTTTTCAAACTGAAGCACAAAATGTTTTAGATGGTGGAGCGAATGTTGCTTCAACACAAGGCTTTAAATGAAGCTTTAAACAAATTTAGAATTTTGATTACTTAAAGATATGAAAAAATTTCAAGCAGTTATTAAAGACCCAGTGGGAATTCACGCACGTCCTGCTTCTATCCTTGCAAGTGAGGCTAGTAAGTTTAAATCTGAACTTAAACTGGTAGCTCCAAGTGGTGTTGAAGGTAATATTAAATCAATTATTAACTTAATGTCTTTAGGAATTAGACATAATGACAACATTACTATCAAAGCAGATGGAGCTGATGAAGAAGAAGCTTTAGCAGCTATTAAAGCTTGTCTTGAAAAAAATAAAGTTATCTAACTTAGCATATTTTAATCAATTAAATCTGTTTATTTTTTAATAGTAAAAACACAATTTAACCTTGTTTCTATTTAACAAAATTGGTTTGAAAAAGGCTATCAATTTTGCTGTAAAAAATTAAGATATTTTTTATCAAAAATTAGCATAAAAAATTGTTATACTAATTAACGTTTTTTATTGAAAATTAAGTATTTAAATTGAACGAACATTCTTTAATTGAAATTGAAGGTTTGAACAAGACCTTTGATGATGGTTATGTTTCTATAAGAGACATTAGCCTAAATATTAAAAAAGGCGAATTTATTACTATTTTAGGCCCTTCTGGTTGTGGTAAAACTACCCTGTTGAGGTTATTAGCTGGATTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTATTGACATTAAAGACATGGCAATCCATAAGCGTCCTTTTGCGACAGTTTTTCAAGACTATGCTTTATTTTCCCATCTAACTGTTTATAAAAACATTGCTTATGGTCTGAAGGTAATGTGAACAAAGTTAGATGAAATTCCAAAACTTGTAAGTGATTATCAAAAGCAACTTGCTCTTAAGCATTTAAAGCTAGAAAGAAAAATAGAGCAGTTACAAAAAAACAATTCTAATGCTCAAAGAATAAAGAAATTAAAGGAAAAATTACAAAAACTTTTAGAAATTAACAAACAAAAAGTTATTGAGTTTGAAAATAAAGAAAAACTACGTAGAGAAGATATTTACAAGAATTTAGAGCAATTAACAAAAGAATGGGATCTACTTTCTCAAAAGAAACTAAAAGAAGTTGAACAACAAAAACAAGCAATTGATAAAAGTTTTGAAAAAGTAGAGAATAAATACAAAAAAGATCCTTGGTTTTTTCAACACAGTGAAATACGTTTAAAACAATATCAGAAGAAAAAAACTGAGTTGAAAGCTGATATTAAAGCAACAAAGAACAAAGAACAAATCCAAAAATTAACTAAAGAACTTCAAACCTTAAAACAAAAATACGCTAATAAAAAAGCAATTGACAAAGAGTATGACAAATTAGTTGTAGCTTACAATAAGAAAGACTATTGAACTTCTTATTGAGAAACATACACACTTCAACAAAAAGAAGCTTTTGAAAAACGTTATCTTTCAAGAAAACTAACTAAAGCTGAACAAAATAAAAAAGTTAGTGATGTTATTGAAATGGTTGGTTTAAAAGGTAAAGAAGATCGTTTGCCTGATGAATTATCAGGGGGAATGAAACAAAGAGTTGCTTTAGCACGTTCTTTAGTAGTAGAACCTGAAATTCTTTTATTAGATGAACCATTATCTGCACTTGATGCAAAGGTTAGAAAGAATTTACAAAAAGAATTACAACAGATTCATAAAAAAAGTGGATTGACTTTTATCTTAGTAACTCATGATCAAGAAGAGGCTTTAGTTTTATCAGATCGGATAGTGGTTATGAATGAGGGAAACATCTTACAAGTTGGTAATCCTGTTGATATTTATGACTCTCCTAAGACTGAATGAATTGCTAATTTCATTGGTCAAGCTAACATCTTTAAAGGTACTTATTTAGGAGAAAAAAAGATTCAGTTACAGAGTGGTGAAATCATTCAAACTGATGTTGATAATAACTATGTTGTAGGTAAGCAATATAAGATCTTAATTCGTCCTGAAGACTTTGATCTTGTTCCTGAAAATAAAGGTTTTTTTAATGTTCGTGTTATTGATAAAAACTACAAAGGATTGCTTTGAAAGATAACCACACAATTAAAAGATAACACTATTGTTGATTTGGAGAGTGTTAATGAAGTTGATGTAAATAAGACCTTTGGTGTTTTATTTGATCCTATAGATGTTCATTTAATGGAAGTTTAACAAGATGCACATTAAGAAAAAATACTGACTTCTGCTCCCCTTCTTTTTATTAATGACAATCTTCTTTATTATTCCAATGGCATGGATTATTGTTAGTGGATTACAAAGTGAAGATGGGGCTAGTATTAGTCAAAAATATGAACCACTTGTTAGTGGCTTAGGTTTTTTTAACAGTTTCTGAACCAGTTTGTGGATCTCAATAGTGACTGTAATTGTTGCATTGTTGTTTTCTTTTCCTTTTTGTTACTTTCTCTCCCAATCAAAAAACAAAATTTTTAAAGCGTTTGTTATTTCAATTGCAACAGTTCCTATTTGAAGTAGTTTTCTTATTAAGTTAATTGGATTGAAAACCCTACTTGATTTATTAATTGGACTTTCTTTAAACAGAGTTGGTGATAACAACTTAACTTTTGGTTCAGGATATACCTTACTTGGAACAATTTATCTGTTTACTCCTTTTATGTTTTTACCACTTTATAACCACTTCTGTGTTTTACCTAAAAACTTGTTGTTAGCTAGTCAAGATTTGGGTTATAACTGGATTTACAGCTTTGTGAAAGTAGTAATTCCTTTTTCTAAAACCGCAATGTTATCAGGAATTGCTTTAACTTTTTTCCCTGCTTTAACTTCAGTTGCAATTGCTCAGTTTTTAGATAACTCTAACCAAGCCGAAACCCTTGGTAACTACATATTTACCTTGGGTAATAATGGTTATGATAGTGCAATTGAAAGAGGCAGAGCTGCTGGAGCAATTATTATTGCTGCTTTAATTACTTTTGCAATTTACTTTACTGTTGTTTTTTTGCCTAAAATTGTCCGTATTGTTCATAACAAATGAAAACAACATGAAAAAGCATTTTAAGAATTTAATTAAAAACAGTTATTTCTTTCTGTTAATAACTTTAATCTATTTACCACTTTTAATAGTTGTACTTGTTAGTTTAAACGGTTCTTCTTCAAGAGGAAATATAGTGCTTGATTTTGGTAATGTTTTAAATCCTAATCCTGATTCTAAATCTGCTTATTTAAGATTAGGTGAAACTGATTTTGCAACACCACTAATAAATTCAATCATTATAGGTGTGATCACTGTTTTAGTGTCTGTTCCTATTGCTGTTATCAGTGCGTTTGCGCTTTTAAGAACAAGGAATGCTTTAAAAAAGACAATCTTTGGAATTACTAATTTTTCTTTAGCAACTCCTGATATTATTACTGCTATCTCTTTAGTGTTGTTATTTGCTAACACTTGATTAAGTTTTAACCAGCAGTTAGGTTTTTTTACCATTATTACTTCCCATATCTCTTTTTCAGTGCCTTATGCATTGATTTTGATTTACCCTAAAATTCAAAAATTGAATCCTAATTTAATTCTTGCTTCTCAAGATTTAGGCTATTCGCCTTTAAAAACTTTTTTCCATATTACTCTACCTTATCTAATGCCAAGTATTTTTTCAGCAGTACTAGTAGTATTTGCAACTAGTTTTGATGATTATGTAATTACCTCTTTAGTACAAGGATCAGTAAAAACTATAGCAACTGAACTCTATTCATTTAGAAAAGGAATTAAAGCATGGGCAATCGCCTTTGGGTCTATTCTCATATTGATTAGTGTCTTAGGAGTCTGTTTAATAACCCTGCAAAAGTATTTAAGGGAAAAAAGAAAGGAAATAATCAAAATAAGACAATGAAAAAACAGTTAAAATATTGCTTTTTCTCACTTTTTGTTAGTCTCTCATCAATATTGAGTAGTTGTGGTTCAACAACATTTGTACTAGCTAACTTTGAATCTTATATTTCGCCCTTATTGCTAGAAAGAGTACAAGAAAAACATCCCTTAACTTTCTTGACTTATCCTAGTAATGAAAAACTAATTAATGGTTTTGCTAACAACACTTATTCAGTAGCAGTAGCATCTACTTATGCAGTTAGTGAATTGATAGAAAGGGATCTATTATCACCAATAGATTGAAGTCAGTTTAATCTGAAAAAAAGTAGTAGTTCAAGTGATAAAGTAAATAATGCCAGTGATGCAAAGGATTTGTTTATTGATTCAATTAAAGAGATCAGTCAACAAACCAAAGATAGTAAAAACAATGAATTACTGCATTGAGCAGTTCCTTATTTTCTTCAAAACTTAGTGTTTGTTTATCGTGGTGAAAAAATTAGTGAACTTGAACAGGAAAATGTTTCATGAACTGATGTAATTAAAGCAATTGTGAAACACAAAGATCGCTTTAATGACAATAGGTTAGTTTTCATTGATGATGCTAGAACGATCTTTTCACTTGCTAACATCGTTAATACTAACAACAATTCAGCTGATGTTAATCCAAAGGAAGATGGAATTGGTTATTTCACTAATGTCTATGAAAGCTTTCAAAGACTTGGATTAACAAAATCTAATTTAGATAGTATCTTTGTTAATTCTGATTCCAATATTGTGATCAATGAATTGGCAAGTGGTAGAAGACAAGGAGGAATTGTTTACAATGGTGATGCAGTGTATGCTGCATTGGGCGGTGATTTACGTGATGAATTGAGTGAAGAACAGATTCCTGATGGGAACAACTTTCACATTGTGCAACCCAAAATTTCCCCAGTTGCTTTAGATCTTTTGGTTATCAATAAACAACAATCTAATTTTCAAAAAGAAGCACATGAGATCATTTTTGATCTTGCTTTGGATGGTGCTGATCAAACTAAAGAACAGTTAATTAAAACTGATGAAGAATTGGGTACTGATGATGAAGACTTTTACTTAAAAGGAGCGATGCAAAACTTTAGTTATGTGAACTATGTTTCACCATTAAAAGTAATATCTGATCCAAGTACTGGAATAGTCAGTTCCAAAAAGAATAATGCTGAAATGAAAAGTAAACAAATGTCAACTGATCAAATGACTAGTGAAAAAGAATTTGATTATTACACTGAAACACTTAAAGCATTATTAGAGAAAGAAGATAGTGCAGAATTAAATGAAAATGAAAAAAAACTAGTTGAAACCATTAAGAAAGCTTACACTATTGAAAAAGATAGTTCAATTCGGTGAAACCAATTGGTCGAAAAACCAATTTCTCCCTTACAACGTAGTAATTTATCGTTATCTTGATTAGACTTTAAATTACACTGGTGATAATATGGAACAACCGTTGTGTGTTTTAGGGATTGAAACAACCTGTGATGATACAGGTCTTAGTATTGTCATTGATCAAAAAATCAAGAGTAACATTGTTATCTCTTCTGCTAACTTACATGTAAAAACAGGAGGAGTTGTACCTGAAATTGCAGCACGATGCCACGAACAAAATCTCTTTAAAGCAATAAGAGATTTAAATTTTGAGATAAGAGATTTATCTCACATTGCTTATGCATGTAATCCTGGGTTAGCAGGATGTTTACATGTGGGAGCCACTTTTGCTAGAAGCTTAAGTTTCTTATTAGACAAACCATTGTTACCCATCAACCATCTTTATGCGCATATCTTTTCTTGTTTAATTGATCAAGATTTAAATAAGCTGCAATTACCAGCATTAGGCCTTGTAATTTCAGGTGGACATACTGCCATTTATCTAGTTAAATCATTTTATGAACTTGAACTAATTGCTGAAACTAGTGATGATGCAATTGGTGAAGTTTATGACAAGATAGGCAGAGCAATGGGCTTTGATTATCCTGCTGGTAGTAAAATTGATAGTCTTTTTAATAAAGAATTAGTTAAACCTCACTATTTCTTTAAACCTTCTACTAAGTGAACTAAGTTTTCCTATTCTGGTTTAAAATCTCAGTGTTTAAACAAGATTAAACAAATAAGTGCTAATAAAACCCGAATTGATTGGAGTGAATTAGCATCCAATTTTCAAGCTACTATTATTGATCATTACATTGATCATGTTAAAAATGCAATTAAAAAATTTGCCCCTAAAATGTTGTTAGTAGGAGGTGGAGTTAGTGCCAATTCTTATCTATCTAACAGAATTAGTACATTAAATTTACCCTTTTTAATTGCTGATAGCAAATACACCAGTGATAATGGAGCAATGATTGGTTTTTATGCATCACTTTTAATTAATGGCGATAAGAATTAAAAGTACAAGAGTTGGTAGATTTGTTTCTGAATCAGTGGGATTAGGTCATCCTGATAAAATTTGTGATCAGATTGCAGATAGTATCTTAGACCAATGTTTACTACAGAGTAAAACTAGTCATGTAGCATGTGAAGTCTTTGCTTCTAAAAACCTTATTTTAATAGGTGGTGAGATTTCAACAAGTGGCTATGTTGATGTTGTTCAAACTGCTTGAAGAATTTTAAGAAATTTAGGTTACAACGAGACTGATTTCAGTTTTTTAAGCTGTATCAACAACCAATCACTAGAAATTAATCAAGCAGTTTTAAAAAATAATGAGATTAATGCAGGAGATCAAGGCATTACTGTTGGTTATGCAGTGAATGAAACAAAGCAACTAATGCCTTTAGGAGTTTTACTAGCACACTCGTTTTTAAAACAAGCAGAAAAACTAACAAAACAATTTGATTTTTTAAAAAATGATATGAAAAGTCAAGTGGTTTTAAACTACAGTTTAAACCAAGTTGAATGTGAAGAAGTTTTACTATCAATTCAACACACTAATGCTATTAGTTTAACAGAATTGAGAAAAGTGATTGAAAATAATGTAATTCTACCTGTTTTAAACCAATATGGTTTTCAAGATAAAAAGCCAACTTGTTTAGTGAATCCTGGTGGTTCTTTTGTTTTAGGTGGACCTATGGCAGATACTGGACTAACTGGTAGAAAAATCATTGTTGACACCTATGGTCCATATGCTCACCATGGTGGTGGTAGCTTTAGTGGCAAAGATCCTAGTAAGGTGGATAGAACAGGTGCTTATTTTGCACGTTTTATCGCAAAACATATTGTAAGTTTAGGCTGGGCCAGTGAGTGTGAAGTCAGTATTAGCTGAGTCTTTTCAAAACCCAATCCACAATCTATTACTGTTAAGTGTTTTAACACTAACATACAGTATGATGAAGTGTTAATTAATAGAGTTGTAAATAACTATTTCAACTGATCGATTACTAAAATTATTGACAAGCTAAAATTACTTGATTTTGTTAAGTATTCTGATTATGCAGTTTATGGACATTTTGGTAATGATCTTTCACCATGAGAACAGCCCACTGAATTGGATAAATTAGAATGCTTAATCAAAAATTTCCATTAGATCCTAATAAAAAAAAGGAACAACAAGGCGTAGTAAAACCAAATCTACCGGTTGTTAAAGAAAAGAAAAAACAGCCATTTAAAAAACCAAATTGGAGTGAATTTAAGCTGTTCAACTTTTTTAAAAAGCACACTTATTTTTGCTTGGTTGTATTAGCTTTTCTTATCTTGATTATTTTACTTAGCAGCTTATTTGCTATTCCACTTAGCCAAATACCTTCAAGTACTGTTTAAAAGAATTGTCCAAATCCACCAAACGGGTTACTACCTGTTTTTAATAGTTTACCCATTTCTGTTGCTTTTAGTTGCATCTTATTTCATTCCTTCATCAGTTTGTTTAACTCATCCATTTTTCTCCCTGATCCTTTTATGATGCGCTGTTTTCTATTAGGATCACGATTAATTAATTTGGGATGTCTCCTTTCTTCTCTAGTCATAGAGTTAATTAAAACCTTTCATAGTTCAATTTTGTTTTCAATTAATTCAGCATTTTCTTCTGATACAGAAAAGTTAGCAGGCAACATTTTTATCAGTGAACTGACACTTCCCATTTTGTGCATTTGTTGCATGTAGATCAAAAGATCTTCTAAATCCATTTTTCCCAAAAACATCTTGCTGATGGTTTTAGTTAAATCTTTTTTATCAAAAACTTGTTCAGCTTTTTCAACTAAACTCATTACATCACCCAAACCTAAGATCCGATTGGCTATCCTTTCAGGATGAAATTGTTCCAATCCATCTAATTTTTCAGAAACACCAATTAATTTAATGGGTACTTGTAAAAGTGAAGCTAATGAAAGTGCAGCTCCTGCTCTAGCATCACTGTCTAATTTACTGATAATAAATCCAGTTAGTTTTAAACGTTTGTGGAACGTTTGAGCAACATTGATAATTTCCTGACCGCTTAATCCATCTACTACCATGATAATTTCATCAGGATTTAATTCATTTTTAACACTTACCAATTCATCCATTAATGTTTCATTTGTTTGTAATCTACCAGCGGTATCACAAATGATTGTTTGACATTTTGCAGTTTTAAAAGCACTGAGTGCTGCTTTTGTTGTTTTAGCAACTGGTTGAGTGCCTTGTGCAAAAAATACGCTGTTAGTTTGTTGTGAAAGCGTTTCAAGTTGTTCAATGGCAGCGGGTCTGTAGATGTCCAAGCCTACTAACATTGTTTTTTGCTTGTATTTCTTTTCAAGTCAATAAGCTAGTTTGCCACAAGTTGTTGTTTTACCTGATCCTTGTAAACCAACCATCATTATTTTTAAAGGTCTTTTTTCATTTAGTTCTTGGTTGGGTTGGCTTAAGATATTAATTAGTTCTGTTTTGATTGTTTTTAATAGAGACTTTTGCAAATCTTGACCAGGTTCAATGGTTTGTCCTACTGTTTTATCTCTAATTGCTTTGATGAAATTTTTAACAACAAGCAGGTTAACATCAGCATCAAGCAATGCAATTCTAATCTCTTTTAGAACTAACTCTACATCTTTCTCAGTGATCGTTTGAGCGTTAATTTTTTTTTGCATCGTGCGCATAACGATGCTTGATAACATTGCTTTGAACATGATTTTTAATTATTTATTATTAAATAATGTTTTAATAAAACAATATTGCAATATGACCCCACATATAAGTGCTAAGAAAGATGACATTAGCAAAGTTGTTTTAATGCCAGGTGATCCATTGAGAGCTAAATGGATAGCTGAGCAATTCTTAGATCAAGCTAAATTAGTCAATGAAGTGAGGGGAATGTTTGCTTATACTGGGCAGTATAAATCTAAAACAGTTACAGTAATGGGCCATGGAATGGGGATCCCTTCTATTGGAATTTATTCATATGAGTTGATGAATTTTTATGAGGTTGAAACTATCATTAGAATCGGAAGTTGTGGTGCTTTAGCACCGCAATTAAAATTAAAAGATCTTGTTATTGCTTCAAAAGCATGAAGTGAGTCTATTTATGCTAAAGACATGGGTGTTGAAATTCCAGAAGATAAGATCTTATTTGCAACAAGTTCTTTAGTGGAATTAGCAAAAGAAACTGCGATTAAGAACAAGCTTGATTTTCATGAAGGATTAGTATTTTGTGAGGATGCTTTTTATCAAACTAGAAAAGATGTAATTAGTCTTGCTAAAGAAAAAAATAGTTTAGCAGTTGAAATGGAAGCACATGCACTTTATGCTAATGCAATCCTGTTGAAGAAAAAAGCACTTACACTCTTAACAGTATCTGATTCTCTAGTAACTCATGAAGCACTTAGTTCTGAATTAAGACAAAAGTCATTTAAGCAAATGGCTTTATTAGCACTTGAAATGACTCAAAAACTAATCTAATCTATGAAATTAGAATACAACCGGATTATTGATAGCACCTTAGTCAAAGCTGATACGCTTCCCCATGAAATAGATACTTTATGTGCTGATGCTCATAAATACCAGTTTTTTGCAGTGTGTGTTAATCCTAGTTATGTTAGTTATGCTAAAAACATCTTGAAAAATACTGCAGTTCAACTCTGTTGTGTTGTTGGTTTCCCCTTAGGACAAACAACCCAAAAACAGAAGGTATATGAAGCTAAGATTGCTATTAAAGAGGGAGCGGATGAAATTGATATGGTAATGAATATTGCTGAGTTTAAAAAACGTTGTGCTTGTGTTATTACTGAAATTAGAGCTGTTAAAAAAGTGTGTGGCAAGCGTAAATTAAAAGTAATTATTGAAACTGCACTTTTAACAAATGATGAAATCAAAGATGCAGTTAATGTTTGCATTGATGGCAATGCAGATTATGTTAAAACTTCCACTGGTTTTTCTTTCCGTGGTGCATCTTTAGAAGATGTTCAGATTATGAATAATGCTGCAGCAAATTTAATTAAAATCAAAGCTTCAGGTGGGATTAAAACAGCAAAGCAATTTATAGATTTATTTCAAGCTGGAGCTAGTAGAATTGGAACTTCAAATGCGGTCCAAATAATGCAAGAATTAAAAAAAATGAACCATGAATATCATTAATTTAATTAATAAAAAACAACGCGGAAAAGCTTTAAATTTAGCTGAAATCAATTGGTTTGTTAATGCTGTTTTAAACAAAACCATTGCTGATTATCAAATTACTGCATTTTTGATGGCTATTTGGTTTAAAGGGATGAACCCAAATGAACTTTTTTTATTAACAAAAGCAATGGTGGATACTGGTGAAATTATTAAGTTTAATCACCATGGCAAGATTAGTGTTGATAAACATTCAACTGGTGGTATTGGTGATAAGGTTTCTTTAGCATTGGTTCCTATCTTAACTAGTTTAGGATTTAGTGTTGCTAAATTATCAGGAAGAGGCCTTGGTTATACTGGTGGAACAATTGATAAATTAGAAGCAGTTGGAGTTAAAACAGAATTAACTGACCAACAAGCACAAGCATGTTTAGATAAAAATGATTGTTTTATCATCGGACAAAGTAAGGACATCGCACCAGTTGATAAAGTACTTTATGGTTTAAGAGATATTACTGGAACAGTTGATAGTTTGCCTTTAATTGCATCTAGTATTATGTCTAAAAAGCTAGCAGTTATGAACGAGTATATTTTCATTGATCTTAAATATGGAAAAGGTGCCTTTTGTAAAACTAAGAAAATTGCTAACGAACTTGCAAAACTGATGCAAAGTATTGCTAAAAGTTTTAAAAGAAAGCTGTCTGTTAAATTAAGTGATATGAATCAAGTACTTGGTAAAGCTGTTGGCAATGTAATTGAAGTTAATGAAGCTGTTAACTTTCTAAAACAAGATTTAGATCAAGTAGGACAAGATTTTATTGATTTAATGCAAACAATTGTTATTAACATTCTACTTGAAACAAAACAAGCAAAAACCAAACAAAAGGCTATTGAACTTTATCAGGATGTTTTAACTAGTAAAAAAGCATGAAATCGCTTTTTATCTTTTATTGAATCTCAAGGAGGAAATGTTGAATTATTTACTCAAAAAGAAGGTTTTTTTAAACCTAAGTATAAGGCATCTATAAAAGCTGAAAAAAGTGGTATACTACATTTTACTGATCCAATTGATTTAGCTAAAATTGGGATTAATCTAGGGGCAGGTAGGATGAAGAAAACAGATCAAATTGATCCAATGGCAGGGTTATTTTTAATGAAAAAAGATAATGAGTCTGTGGCAGTTGGAGACACTGTATTAAACCTGTATAGTTCTAGTCCTATTAGCAATGAATATATCTCTGCTGCTCAAAAAACAATAATTATTAATAAATAAAAATTCCTATGAAGGTGAATTTAGAGTGGATAATTAAACAGTTACAAATGATAGTTAAAAGAGCATATACTCCCTTTTCTAACTTTAAAGTTGCATGTATGATTATTGCTAACAACCAAACTTTTTTTGGAGTTAACATTGAAAATTCTTCCTTTCCAGTAACTTTGTGTGCTGAAAGAAGCGCCATTGCTAGCATGGTTACAAGTGGTCATAGGAAAATTGATTATGTTTTTGTTTACTTCAATACTAAAAATAAGAGTAACTCACCCTGTGGAATGTGCAGACAAAACTTACTGGAATTTTCCCATCAAAAAACAAAGCTTTTTTGTATTGATAATGATAGTAGTTATAAACAATTTTCCATTGATGAATTATTAATGAATGGTTTTAAAAAGAGCTAATGGATAAACTTAGATTAGAAGTTGAAAGATGGTTAAATCATCCTAATGTTAATTGGGAGTTAAAACAACAAATTAAGGAGTTGAATGAATCAGAAATTCAAGAACTTTTTAGTTTGGAAAAACCTTTATTTGGCACTGCAGGTGTAAGAAACAAAATGGCACCAGGTTATCATGGTATGAATGTTTTTTCTTATGCCTATTTGACCCAAGGTTATGTTAAGTACATTGAATCCATCAATGAACCAAAGCGTCAACTACGGTTTTTAGTAGCACGTGATACAAGAAAAAATGGTGGTTTATTTTTAGAAACGGTTTGTGATGTAATTACATCTATGGGTCATTTGGCTTATGTGTTTGATGATAACCAGCCAGTTTCAACACCTCTAGTGTCCCATGTCATTTTTAAATATGGTTTTAGTGGAGGTATTAATATCACAGCTAGCCATAACCCTAAAGATGATAATGGTTTTAAGGTTTATGATCATACTGGTGCACAGCTTTTAGACACACAAACAAACCAATTGTTAAGTGATTTACCTTGTGTTACATCTATGCTAGATTTGGAATTACAACCAAATCCAAAGTTTGTCCATACTCTTGACAATGAAAAGGTTTATAAAAACTATTTCAGAGAGTTGAAAAAGGTGTTGGTTATTAACAACAACAATTTCAAAGACATTAAGGTAGTTTTTAGTGGGCTTAATGGGACTTCAGTTTGCTTAATGCAACGCTTTTTAAAGTACCTTGGTTATAGCAATATTATCAGTGTTGAGGAACAAAATTGGTTTGATGAGAATTTTGAAAATGCTCCTAACTTAAATCCAGAGTATAAAGATACATGGATATTAGCACAAAAATATGCTAAGAAAAATAATGCTAAGTTAATTATTATGGCAGACCCTGATGCTGATAGATTTGCAATTGCAGAGTTAAATAATAATCAATGACATTATTTTTCAGGTAATGAAACAGGAGCAATTACTGCTTACTATAAACTTAATCATAAGGTTTTTAAATCACCTTACATTGTCTCAACTTTTGTCTCAACTTATTTGGTAAATAAGATTGCTAAAAGATATGGCGCTTTTGTGCATAGAACCAATGTTGGTTTTAAGTACATTGGTCAAGCAATTAATGAGTTATCACAAACAAACGAATTAGTTGTTGGTTTTGAAGAGGCAATTGGTTTAATAACTAGTGATAAATTAAACCGCGAGAAAGATGCTTATCAAGCTGCTGCATTATTGCTTGAGATTGCTAGACATTGCAAAGAACAAAACATCACGCTTTTAGATTTTTATAAAAGAATTCTTTCTGAGTTTGGTGAATATTTCAATTTAACAATATCTCATCCCTTTAAAGCTACTGCTACTGATTGAAAAGAAGAGATTAAAGCTTTATTTAATCAACTTATAAATGCTAATTTAACTGAAGTGGCTGGTTTTAAAGTAGTTAAAGTCCATCTTGATAAACAAACAAATATCTTAGAGTTTGGTTTTGAAAATGGCTGGGTTAAATTCCGCTTTTCAGGTACTGAACCTAAATTGAAATTTTACTTTGACCTAACTAATGGCACTAGAGAGGCTCTAGAAAAGCAAGCTAAGAAAATTTATAAATTCTTTGTAAATTTACTCAAACTCAACAAAGCTTAAATTTTCTAAAGGTAAGTTAATTAATTGGGTTTTAGATGTTAATTCTATCTCTACAACTGCTTTGTTAACTGTACCTTGATAAAGTGCTTTTATCTGTCCTTGAACATGAATAGTATCAGTTAAATGTACCTCTACTAAATTACCAACACTAAATTCAAAGAGTTGTTCAACTTTATTGTCAGTATCAAGAATTTCATATTTGCCCAAAAAAGGAACTAATACTGATGGATCAAGTTTGGTTTGCTCACTTTGAATAATCGTTGCTTTTTCTTTCCATTCATCTTGTTCTTGACTATTTTGTAGTTTTTTAAAAGCTTCTTTTAGTTTTTCAGCATCAACAATTTCTGAATCTTCATCCTCTTTTGGTTTTTGAATGGCTTGTTTTTGTTTTAAGATATATTGAAATTTTTCATCAAATTTATCACTGTCCATTTCAATAATGGCAGTATTTGTTAGCATAATTCGTTTGCTAGGATTGATTGATATCCCTTTAAGCATTTGTTCTAAATTATTAGCATCTTTTTCATCTAAAGGGATAGGTAAAGCACCCCTGCCAGAAGAACCTACTATGCCAGTGATCCCAGGGAAGTTACGAACAATTCTTCAAGCATCTTCACTATAAATCATCTTTATGTAGATGTATCTACCAAGGAGATTTTTTTCACTAATTTTTACCCTAAGATAACGGTAATCATCAAGAACAAACCATTTGTTAAAAGTAGTGTTCTTTAAGGAACGAGGAAGTTTTCCTGATTTTAATGAATAAACTTCTTCATGAACTTCCCTTTCTTTTAGAACTTTAACATCAACAATCTCATGATTAAATCCTAAAGCTTGAATTTTAGCTTTTAGATTTTTTACAACAGCTTCATCTTTAATACTAACAGGAGCTACATACCACTTTGGTGTTAATTCACTAGCTTGCATAAATTAATTTTTAAATATTCCCAACGTAATTAACAATTGATTGATACCAAAAAAGATTCCTACCATCAGTCCACTAACTAGCAAAATTAAAAGAAAAATTATGATAAGTTGTCTACCTTTTACTCAAACAATTTTGTTGATCTCTTTATCAACTCCAAATCATAAATTAACAATTCGTTTTTTAAAAGGAAGCTTGGGTTTCTTTTCCTTTGCTAAACTTTCATGCTTTTCTTTTTCTTTAGTTCTTTCCTTATCTTTACTTTTCTTGGCTTCTGTTCTAAGTTTGAGCTGTTTATGTAACTCATGAATTGAAGCATCATCATAAGCAGTTAGCTTTTCCTTCTTTTTAAAGCTAAAAGGCAGTTTTTTTTCCATGGCTTTTTAAAAAGAATCTAGATGCTTAGTTTTTTGATTGCACTGCTTGCAGTACTTGTTGATGATAAGTCGCTGCAAAGGTTGTTTTGTTCACCGTTTTACATAGTTACGACTTAAACAGTCTTGACAGACAAAAATAATCTTTTTGCGCAACTTCAGTAGCAAATTATAAATTATCTGCTTGTTTTACTAATTTAATCTTTTGATAGCAATAGAGCTGTAAAAGGCCAAAGGGTAAAAAACAAGATCAGTGTAGAATTTTTTTGAATTTAGGTTCAATATTTTCACATGCAGTATTAATTGTTTTTCAAATCATTATGCTGATAACTAACTGGATGATCATAAAACAGATAGCAAAAACTCCTAAAACAATTAAAACTGCTCGTAAATTAAAAAAAATAGGATCACTTCTAATTGCACTTAAACTAGCTTTTCTAATTAAATCAAAGATGATACCAATCCCTACTAAAGAGATAATACAAACTAAAAAAAAGATACTAATTAACAAAAGAATCTCATTAAGGTTATGGAGTTTTTTAAATACTTTCATGGAAAAGTGTATAGAGCATACTTGATTTTAAATGCATTTTTTTAGCTAAATAATTGCATGCATCTTTTAACTTAACACCAATATCCATTAACTTCTTTATTTCATATACTAAGTATTGATTGGAAGATAATGTTTGGTGATTAATATTTTGATTATCAATAACAATTACAAATTCTCCTTTTAAAGTGATGTCAGGTAAAGTGTTTTCACTGGTGTTAAATCAATAATGTGATTCATGTAACTTAGTTAATTCTCTTCCTATAAAAACGTCATTGTTTTTAAAAACATTTTTCACAGTTTCTAAAGTATTTTCTAACCTATGCACTGCTTCAAAAAAAACGATAGTGCTTTTCTGATTTTGATAGGTACTTAAATAATTTTTGAGCTGATTTTGTTTGTGACTTAAAAAACCTAAAAACAAAAGTGGTGTTGTTTTAAAACCACTAGTGATCAATCCACACATTAATGCACTAGGACCATTAATAACTTCGATTCTTATCTCCTTATTTTTTGATATGATCCAATTAATCATTTCATTACCAGGATCAGATAAACTAGGATAACCTGCATCACTAACAAGACAACACTTAAAGTTAGTTATAAATTCCTCAGCAAAAGTTAAATTCTGTTTTTCTTTAAAACTGTTGTTAATAACAAACTTTTTTTGCTTGCAATCAATGTTGAGTAAATCCAGCATTTTTCTTGTTACTCTACTATCTTCACAGAATAACACTTCACAATCTTGTAACGCTTTTTTAGCTCTTTCACTTATCTCTTGAATATTACCAATTGGTGTAGCAACTACTTTAAGTGTTTTCATACATCTTGAAAAGTTAGATTTAATAAGTTAAGTTTTTTAAATAGTTGCTTGTGGTTAAAATAAGGTAAATTAAGATTATTGCATAAAAGTAATCGCTTTTTCTTATCATTTAACTTTAAGCTTAAATACTGATCTCATAATATGCTTTGATTAGCATTTTTTGTGATTGCAACACGATTATTTAAAGCAGCAATTAAAGCACTAGTTTCCATTTCAGCAATACCAGCTTTTTTAGCGTTTTTTAACCTTGCATTCATATCAACATAACAGTTGTAATAGTTTGTTAGATGTTGTTCTAATTTACTTCTAATCTTTTTGCCTTGTTGATCTGGATCTAATAACAAGATAACAGTTTGCTTTTCACTGATTTTTTTAATTAGGTTAATAGTTTCTTTTTTTAAAGCTGAACCATTTGTTGTAATTACATCTACATCAAAAATTTGCTGTAATTTAGCTTGGTCAGTTTTACCCTCACAAACAATTACCCCATCAATTTTTATACGTGCTTTTTGATCCATTCAATGTGACGTTCATATATTGCAGAAACTGATAAGCTGTTAGCGTAACACAGAACAACCTCTTCATATAAAGATGCTAGATCAACTACTTCAAACTGCTTTACAGCTTTAAACTTATATTGAGGAATAGAGTTTGATACAAACAAGAAATCAATTAGTTTTTGATCAAATGCTTCCATAAACTTTTGTTCTGCATCATTGTTAAACAAACCATGAGTTGCCATTACACACACTTTTTTAGCTTGTTCTTTTTGTAATAGCTTGGCTGCTGCAATTACTGTACCACCAGTATCTATCATGTCATCAACTATTAAACAGTTTTTATTTTTCACTTCACCTAAAACATTAATTGATTCAGCAACATTATGAGATGGTCTTCTTTTATCAATAATGGCTAATGGTAGTTCTAGTGTATTTGCAATTAACCTTGCTCTTTTAACCCCACCATAATCAGGGGAAACAACCACCAAGTCTTTTTTACCAAGTAGTTCTATAACTCTAAAAAGAAAGATGTGATAAGTTCTTAAAGAATCAACGGGAATATCAAAAAAACCTTGGGTTTGATCACTATGAATGTCAGTTAGAACAACCCTGTTAGCACCTGCTTTTGTTAACATATCAGCAATCAATTTACTGGTAATTGGTTCTCTTCCTTTTGTTTTTCTATCTTGTCTTGCATATCCATAATAGGGTAGAATGGCAGTAATACTTTTAGCACTACCTCTTTTCAATGCATCAATAGCAATTAAAAGTTCCATTAAGCTATCGTTAACATTAGGACAGGTTGATTGAAAAATATAGATATCTTTGTTACGAACTGATTCATCAAAACGGATATAAGTTTCACCATCAGCAAAGTGTTCGCAAACCATTTTGCCCTCTGAAATATTTAGTTTTTTGCAGATATTTTCAACCAGTGTTTTGCTTTTAGATAAACTAAAAATAACGTGCTTTTTATTATCGATACTCAGCTTCAAGCTCGCGTCGGATTGTTTTCTTTTTAATAGTTTCACGTTTGTCAAATTTCTTTTTAGGTTTTGCCAATCAAATTTCCACTTTAATTTTGCCATTTCTAAAGAATACTTTACTTGGGATTACAGATAAAGATTGTTGTTGTTTTTTATTGATAATTTGTTTAATTTCATGTTTATTTAATAAAAGTTTCTTAATTCTATCTGAAGCGTGATTTAATGGACCTGCAAAACTATAAGGTGGAATAGTGAACTGTTCTAAAAAAAGCTCATTGTTTTTAACAAAAACATAAGCTTCTTTTAAACTACCTTGACCTAAACTTAAAGCTTTAACTTCACTTCCTTTTAAAACTATTCCAGCACAATAAGATTCCATTAAATGATAGTCATATTTAGCCTTAGGATTGTTAACAAGAATTAACATTTATCAATGAAATTATCTGTAATTATACCTACTTACAATTGTGCATCATTTATTGAAAAAGCAATTAATTCAATTGTTAAAAATAGACCTAATGATTTGGAAATAGAAGTTTTAATTATTGATGATGGATCAATTGACAATACTAACAAAGTTATTAAGAAAATTCAAGACCAAATTAATAATTTAACTTTGCAGTATTTTTACAAAAGTAATGGTAACTGGGGTAGTGTTATTAATTATGTTAGAAACAATAAACTAGCAAAAGGGGAATGAGTAACAGTATTGGATAGTGATGACATTTTTTCAAAAAAAACAATTTCTATTTTTCAAAAATATGCCCAAAAACAAAGATATGATGCGATTATTTTTGACTACTATAAATGCTGAAAAAAGTTTTTGTGAAAAATTCCTACCTATGCAAGGTTTAGAAAAGAAATTAAAGGTGAATTGAAAAAACAAACACCTTTTTGTATTCCCTTAGCTAAGTTTTTTAAAAATGAGGTTTTCTATCAACTTCCTAAACTAAGAGAAAATGTTGGTTTTCAAGACGCTATTTATACGATGCATGCATTACAAATTGCAAATAATGTTTTCCATGTTTCTAAAGCTGGAGGATATTACTTTTTTAAAAGGGTAGGTAACTCTATGAGTATCCCTTGACACAGTTCTAGGTTTGATATTGAAGTACAAATCTGCAAGGATCTGATTGAAAATAATGCGCAAGAGATCGCTTTAGTGCATTTACTTCGTTTAAAATTTCGTAATTTAGTTGATGATAAAAAGATTAAATTTACAGTTAAAAGAGACTTTTGTTTTAGTGGTTTTAGTTGGTATAGTAGGTTAATTTTATCTCTGATGTATAACTTCTGATTGAAACGTTATTTCAACAGTTCTGAATAACGATTTTTTTGCTTATTTGCAATTACAAACTGTCATTCATTGAGTTTTCATTTTCTTGTCATTTCCTGAACCAATTGATCACCATCTTTAATCCAACAAGCAGGAAAGAACTTAACAATTAGGTTTGCTAGTGCAATAAGTCCTAAGAAGAAAACAATACTTACAATTACCCCTGGTAAAATACTTGTACCTTTTCCTTGAATTAAAACAGGCGCTTGGAGAAATACTGATTGGGTAATGTCAAATAAGGTGTAAGCAATATAACCAAAGCCCCAACAAAATCCAAAAAGAATAGGAAGTTCATTCTTCTTGTAATCTTTAAATTCATATGGCAGGATTAAATTTGAACTTGATAAACTCCAAGCAAATGCACCACCGATAAATGAAAAGATACTAATTAAAGCAAACCCAGCTGCACTACCAATACCAAGTGTTGCTGCAAACATGATGATTATAAGCAATACCACTATGTTAGCTGTTAATAAGAAGTGCATTCACCTCTTCTTGTCATAAATGGTTTTATTAAATGGTGAAAAAACAACAAAGCCCAAAGCATAACCAATAACTCAAAAAATAGCAAGTGTTGCTAAACCTGTTGTGTACACACCAGTACTTATCAAACCATTTGATCCTGATGGGGAAACAGTTTGCAAGATGCTAAATCAAGCTGGAGTTAACGGATTGACTATTAGTATTAAAACTATGCCATAAACACCAATTAATTTTCATGTTGTTTTATTCTTTAAGATATCTATGGATTTTGGTTGAGTAGTTAAACTCATATTTTCCTTAGTTTGCTTTTGAGGAAAGATGTGATCTATCTTGCTTTCAAACCATAAAAATAAACATAAGTTAGCAAACACAACCAAAATCATCACAGTCATAATGTAAACCCAGTATTGAGAGGCTACTTGTTGAACTTGTTCAAATAAAAACGGTGTGAAAACTACTGCAATCCCAATGTTAAAACCCCAAAGGTTAGCATTAGATAGGATTGATTTTTTTCTGTTTGAAGAAAGATTAGCAATTGCTGGTTGAGTATAAACTACTAAGGTAGTTCCACCAATAGCAATTGTGCTTCTAAAGATAATAAACAATGCATAACCAGTAATTGATGCAGCTGTACCAATACTATTACCATCCAAACCCTTAATAAGTTCAACACTTGTTCCATCTGCAAGCATCGTTTTACCATTGATCATCACCGGTCCAAGTAATTGACCCACTTGTAAATTGCTACTTAGTGAACTTAATTGAGTTTTTACTGAATCTGATAAAGGTCTTAATAAAGTTAATTGATTATGACCATTCAAAGGGTCTCCTATAATAAGAAAGGGAAAACAAACACACATGATTCCCATCATGATTAAAACCGCATAACGATAACCAAATTTCAAAACAACAACTCCACAAAGAATAGAACCTACTGCTCTCAATAAAGTGATAGTTCAGTTGGTGGAAGATGTGGCTATTTGCCCAGCATTTTCAGTAAAGAAAGAACTTAACCAACCACTGTACTGTGGTAAAGTGGTGGTTGATTGAGTAAGTATACCAGTTGGTTTACCACTAATTCGATCAATTACAAACCATTCAACTACAAACAATAGATACCCAAAAATTACTATGATCCACAGGGTAATAAGTTTCAGATCACTTACTTTTTTTTGACTTTTATTTTCCACAAATTAAGACAGAATTTACATTAGACTAATTTTAAAAGCTGTAGCTAATTGTTTTTTAGAACAAACTGAATCAAAGAATTTGAAATTGTCTTTCATTGTTAACAGCATTTAACAACAAAATTGAATAAAAATAAAAAACAGACCCTGATGGTCTGTTGGATATTTAATGGCGGAAGCGGTGGGATTCGAACCCACGCACCGTAGAACGATCTAACACCTTAGCAGGGTGTCCTCTTAACCACTTGAGTACGCTCCCAGTTTGTTGTTAAATTTATTATAAATATGCAACTAATTAGCGCTTTAGATGTTTAAAAATTTATTAAGACCATCCCTATTTTTTAACTGGAGCCAAAAAACATTTAAAAATAAATTTTCGTTTTTAAAACAAGCAGCAAATGCATTACAAAAACAGGCTGTTATCAATGATAACAATGTTGCATTTGAAGCTTTAAAAAAGCGTGAAGAAGAGATTACAACTGGAATTATTACTAGTTTAGCTTTACCTCACTTACAAAGTCAAAGTGTTATAGAACCTTTTGTTGCTGTATTTAAGGTTAAAAACTTAGATTGACAATCATTAGATCAAAAACCAGTTAAATTGATATTTTTAATTGGTGTTCTTGTTGACAAAACCAATTTGCATCTTGATTTCATTAGTAACTTTTCCAAGTTAATGTTGAATGAAACATTTGCAAGTAAGGTTTTAAATGTCACTAGCTATAACGGCTTGATTAAACTAATTGATCTTTTTAACCAACAAAAAGTGCAAGACCAACCAGCTGTTGAAACAAAAAAAGAGTATGACTTTGTTGCTGTAACAGCATGCCCAACTGGCATTGCCCATACTTTCATGGCTAAAGAAGCGTTGGAAGCATTTGCAAAAAAGCATAACTTATATGTAAAAGTTGAAACTCAAGGTACAGATGGGATACAAAATCAACTTACAAGTGACGATATTAATAACGCTAAAGGTGTTATTCTTGCTTGTGATCGTTTAATTGATTTCTCTAGGTTTTATGCAAATAAGAATGTGATTGAAGTGTCAACTACTAAAGCCATTAAAAAACCTGATGAGGTGTATGAATTAATTAAAAACCAAAAGGGTAAACAGCTTGCAAATAGTGCTAAACCAACTAATCAAACCCAATTAGCTGAAAGTGAAGGGGAATTCAACTTTAATAATTTTCACAAGCGGATTTATCGTGCCATTTTAAGTGGTGTTTCTTACATGCTACCATTCGTTGTTTTTGGTGGGATATTAATTGCACTCTCTTTTCTAATTGATATAAATAACGCTAATAATGCTGGTGGTAACTTTGGCACAATTAATCCTGTTGCTAACTGGTTAAATAAGCTAGGAGGAATTTCCTTTAGTTTAATTGTTCCTATCTTATCTGCATACATTGCATATGCATTGGTATCAAGACAGGGATTATTACCTGGTTTTGTTGTTGGTTTAATCTCTTCAGGACAATTTTTATTAAATATTGTTCTGACCAATGGAACAATTGAATGGTTAGCACCATCACAAGTATCAAGTGGCTTTTTTGGTGCTATCTTTGGAGGACTTTTGAGTGCTTGCTTAATAATTGTTCAACAAAACTACATCTATAAAAAATTACCTCAATCTTTGCAGGGAATTAAAAACATCTTGTTTATTCCCTTGTTTGGTACTTTATTCACTGCTGGTTTATTTTGGGTTATTAACATTCCTTTAATTTATCTAAACTATGGATTAAGTCTGTTTTTAAATATTATGAACAGCCCTATCCTAGCACCTTTACTTGGTTTTGTAATTGGGTTGATGATGTGCTTTGATTTAGGGGGGCCAATTAATAAAGCAGCTTATGTTTTTGGTGTTGTTTCTTTACAAAATCAAAATGCAGGAACAATTTCGATGGCTGCAGCTATGCTATCAGGGATGGTACCTCCTTTATCAATTGCTTTGGCAGCTTCCATTCGAAAGAGCTGCTTTGATAAACAGGAATTACCTGCAGCTTATGCTTGTTATCTGATGGGATTGAGTTTTATTAGTGAAGGTGCTATCCCATTTGTTGTTAAAAAACCTAAGGTGATGTTAACTGCTAACTTAATTGCTGGAGCAATTTGTGGAGCATTAACAGGAGCATTTGCCTTATCAATTCGTGCTCCTCATGGCGGTGTTTTTGTGTTTGCACTTTTAAAAACTACTTTACAAGGGATTGAAGGAGCTACATTACAAACTGGAGTTGGCATTGGTTTGGCATTGGTTTGTTTAATAATTAGTATGATAGTTGGTAGTAGTATTATCATTGGCTATGACTTGATTGCAAAACATAACCAAAGAAAGCAAAATCTGAATAGTTAATTACGCTTGCGCAATTGATTATTATGTTGATTTAAACAAGCAAAAAAATAGTGTTTTAATACCTGGTGGTAAGGGGATTAATGTTGCTATTGTAATGAAATCACTTGGTTTTGATCCAACTGTCATTACTTTTTTGGGACAACCCACTAAAAACTTATTTTTAGAGTTGGTAAAACCTTATGATCTAAATATAGTTAGCTTCATTTCTGAAACTAAAACAAGAATTAACCTTAAGTTATTAAAAGATGAAAAAACTACTGAAATTAATGATTTAAGTCCTTTAATAACAGATGCTAATCTAACTGAATTGTTAACTTTTTTAAAAGCTAATGTTAAGAATAATGATTTGGTTATCATCAACGGAAGATTTAAATTTGAAGCTTTAGAAAAAGTTCTAAACTTGGTCTTTACATTAACAGAAAATGTGGTTATAGATGTTGATGAAAGCAAAATGTTAACGCTTTTAAATCAGTCTAAACCACTAGTTATGAAACCTAACATTGATGAGTTTCAAACTATGATTAATACTTTTTTTCACGATCAACAAAGCTTAATAGCAGCAATTAAAAAATTTCATTACTGTAAGCTCTTATTATTATCTGATGGTGACAAAGGAGCTTATCTTTTTGATCAGAATAAGTTATTGTTTGTAAGTTCTATCACTCCTAAACAAGTAGTTAGCACCACAGGAGCAGGTGATACTTTGTTGGCAGTTTTTTTAGCAAATTTGATTCTAAAGGTAGATTTAAAAACTGCTTTGATTAAAGCAACTAACTATGCAAGTGCAACAATTAGTAAGTTAGGTGTTGTTGATAGTAAAGACAAAATTAGTGTTATAACCCCAAAAAGTTACTATTTATAATTAATATTTGATGGTAAAAAGAAAGCGAAAGCCTAAGCTTAATTCGCGTAATATTTTAACTATCCAGATTGTTTTAACAATCTTTAGTATGATCTTTTTTCTTACTTTGCTATCTTTAATCTTGTTTTTGAGTTTGCAAAGTAATTTAGCTACAGCTTTAGTTGAAAACAGAAATAAAGCTGTGGAACTTGTAGATAACATTGTCTTTTTTTAAAAGAACTGATTTTTAAATTTGTCTCTGACTAATTTATTAGTGTGTTAAACCTAATTAAAAATGTTATTCGTTCTTTAAAGAGTGCTAAGATTGCTTTAATAGCGTTAACTTTTTTAATTTTTGTTGCTGTTGGTGGTTTTGTGTTGTTAAATAACACAGTTAATAATTTTAACGCTGCTTTTAACTATGTCACCCACACTGGTAAATTAAGCAATGCCATCATTAATGAGCGTTATGACTTTGGTAAATTAGAGTTTCAAGAACAGACCAATAATTCTCAGAATAGTAGCGACAGTTTTACTTTAACTTTAACTAATGATTCAAGAACAAGTTTTATTAATAATGCCTTGAGAACTAACCCTTCTTTGTATGAAGGATTAGTAACCCAAACTTTTAGCTATCAAAACAAAACTGAAATGACTGAAAAAACCAATATAGTTAATCAGTCTAAAATTATTGCTGCTAACAATCTTAACAATGCATTAAGTAAAGATAAACAGCTCTTAGTTTCAGGTCAACTTGAAAAACTAAATGCTGTTTTTCGGGAATATAAAGCTATTAATATTACTGACAAAAGTGTTTTTAAAAAATTGATAGTATCAGAACCTAATGATTTGGTAAATAGCCTAGTTATTTTTGATGGTCAAAATTTATCTAGCTCCAAACAAAGTGATTTCAATAATTTTTTAAATCAATTTAACGAAATTAAATCAAAGGGTAAAGATAATTTAAGTACTACTTTAAAAACTGGGCAGTATCAGGCATTTTTACAAACTCTTTTTGATTATGCTCAAGCGAGTGAAACAACATTAAAAGATCAGCTTCAAAAGTTAATTTCAAATCCAGATTCAAGTGAAACAAATCAGGTTAAAAATCTCTTTGATACTCCAAGTACGCTTACTAATATTGGGGGTCAATTAACCTTACAATGAACAGAAAATAGCCTAACAAAACAGATAGTTATCTTTGATCCTAGTAGTTATGAAACAATAGTCGCTCCTGGTAACTGAACTTATCAACAACAATTAGGTAAAGAAGTTTATCCTGATATTAACAACTGAGAAAGTATTAAAAAACTACCACTTGAACAATTTGAAAGTGAATTTTTAAAAATTGATCAAAAGTATAAGATCAGTATTGATAATATCGATTATTTAGTTATTGGGGTTGGAATTAGTCCAGATTTTGTTTATCCTGTTTTTAGTGCATCTTTAATTGTTCCTAACATTGAAAATGAACAACTTTACTATGTTAACCAAACTGGATATGAAAGAACTTTTTCCTCTTTTTTAACAAATCCAGTTGAAACAGCAATAGTAGCAAGATTGATTAATCTTGAAAGTGATCTTAATACTATCAACCAGTGAGCTGTTGAAAACATGTCATGACCAACAAACATTAAAGCTGCATACAGTAGTTCTGATACCACTAATATTTTGAATTTATTAGCAGCAAGAACAGTTTTTATCCCTAATCTAATTAACACAATTAATTTAGTGGCTTTGTTTTTAACTATTGCTATCCTAACTGTTGCTATAATTGTCAGCATTTTAATCCTGATTAGTTATTTAAAGAAAAACACTGAGCAAATTGGCATTTTAAAAGCAAATGGGTTAAGTGGTAAAAAGATTAACCTTAGCTTGTTAATCTTTGGGTTAATTCCTGCTATAGTAGGTGCTATTTCTGGATATAGCTTTGGAATTGGATTTCAAGACGTAGCTATTCATCTATTTAGTAACTATTGATTTATACCAACAGCAACATCAAGTTTTTCAGTAGTAGGATTGTTGTTTTTTTCACTGTTTGTTATCTTAATTATGAGTAGTATATCGCTTTTAGTGGGATCAATTATCTTAAAGAAGGATGTTGTAAAGATTTTAAAGCATGACAGTGAATTTAAAGTTTCAAGATTAGGACTTAGTTCTAAGAAATTGTTTGCTAGGTTTGGTATTATGACCAGGTTTAGAGTAGCATTAGCATTTAACGCTCCTTGAAAATTAGTTTTTCTAACCTTGATGAGTTCATTTACAATGATGATTTTAAACCTTAGTTTTGCAACTAAAGATAGCTTTGAAAATGCTCAATCAAAAACTAATTTAACTAATCAGAACCACCAATATGAATTTGAACTCGCTTCAGCAACAACACAAAGTGGTTTATTGAAGTGACAGTTATTTGCAGAACTAGGTACAACTGATAAAAGAAGTGAAAGTAGTGTAAAGCTTGCAAATAAAAGGATGGATATTAGTAATGTTGATGCATCTAAAGATTGAAAGAACCAACAAGTAATTAATTTTTTAAGCGATGCTAGTGGCTTTAGTAATGATTTAAATTACCTTGAAAACATTGTTCAATCCAAGATAGGTTTAGACTATTCATTGGGATTTAACAATATTGTTTCAAATCCCTGAAGGTTAAGTGAAACATTAATGCCAACTAACCAAGCATCTGCTTCCAACACTGCTTTTCAAAATTTTTTAAAAGCAATCATTACTATAAATCCAAGCCAAGGATCGCAATTCATTAAACAAACCCAAGATCCATTAACAAAAAGATTTATCTATGCAATTGACAGTGATAAGGCATTAAAAAATAATAATGAACAAAACGGTTCCCAAAACCACTTAACTTTAAATGATGATTTTGCTAAATTTCTCTACAGTCAATTTGAATTAATTAAAAAGAGTGGGAATGCAAGTAATGAAGATTTAAATGCAATTGATTTTGAAAACCCCCAAACAATCAGAGATTTTTACAACAAGTACAATGCTTTACCACCATTAGATTACAAACTTAGCTTTAATGTAATAGGTTTACCCAAAGAGACAATTGCTGGACAAATTGACACCCCTAAGTATGGATTTTTAACCCTTCATGGTGAATATCAAAATACTCCTATCAAGATTAAAGGTATTAAAGATTGAAAAGATAAAGTGGATAATTTAGGTCCAGTTTTGAGTGATCAAAACAACCACATTATTAATCAAGAATTGTTTAAAAATTATTCTTTTGATCCTTTGATAGTTAACAATTCTGCTGCAAAAAAATACCAACTTGCAATAGGTAGTGAGATTAATATTGCAGTTAACAACAGCTTCAAACGGATTGACAATAAGATCATTAATCAAGATCCTTTAGTGAATGCTACCTTTAGAGTTGTAGGGATTAACAATTCCGCTCATGATCCTGAATTTTTCACTAGTTATAGTACTGCTTTTAAAGTATTGGAATATCCCAATGAATGGTTCGTAAAAAAACTTCCATTTAATAGCTTCTATGCTAATTCGCTTTTAAGTTTTGTTCAATCTACTTCGCTATTTTCTGAATCTGGTATTTTTCCTGCTACTAGTAGTTTTTCAACTAATAACACTGTACTTGTTGAGTTAATTAAAAAAACCATTAATTACAAGAATGGTCAAATGAATCAAACTTCAAGTAATGACTCTTCTAAGAAAGAAAATTACCAAAAATTGCAAAAAGCATTAGGAATATCAACTGATTTGGAGATTAGTAAAGTTAATGAATATGTTGCTATCTTAGCAAGGGTTTATAATGGTTTACCTTACAACTCTACTATTAGCTTTATTAGCAATGTTGCTGCTAACAACGCTTTATTTGGAAATATTGCTAACACCACCAAGCAGATTCAAGCTGTTGTAATTGCAGTGATTATTCCTATAATCATGTTGATTATTCTTTTGGTTTCAACTACCTTAATTCAAGAGTTGAAAAAAATTGCTATTAGATTAAAAGCATTGGGATATTCCAATTTAAAAATTCTCGCTTCATTTTTATCAATATACATCCCTTTATTTGCCTTTGGTTTGTTGATTTCTATCCCCTTTTCTATCTATCTAATTGCACTACATAATGAGGTAATTTTTGCAAGCTCATCGATCTTTTTAGATGCTTTTTTAAGTTTTGAAAGTGCAATTGGTTCAATGTTAGTTTTACTAGCGGTTTTATCAATTACCTTTGTGTTGAATTGATTAGAGTTGAACAAAATTAAGATTGACAAGGAAATCAAAAACTCCTAATGGATTTTTTCTCTTTAAACAAAATCATAAAACCCAACCAGAAATTCACTAGTAATGAAGCTGAATTTCTACAGATAGCTACTGATTATTTGGAGGAAAGTCAAAACTATCTTCAAAAGGGTTTAAAGCAATTAAAAAAAGAATATAAAAGATCCATTATTTATAACCCTAACCTTGAATATAAACGCTTTGTTAAATGAAAAGAAAATTTCACTGAAACATTTGAAAGTTATTATGACAGGTTTTTTATTACCAAATACAACCATTATTCACTAAGCTTACTTTTTAGCTTTATTAATGAACAGATTGAAACAGTTATTGCTAGTTACAACTCATTTCTAAATGAGCATAATAAGTTAGCTTTTAATAAAGTTAGTTTTAGTTTTGAAAAGAAACTTTTTGAAGCTACACAACAGTTTAATAACTTAGAAAAAAACACTGCTATTAGTGATGATTTACCGCTCCAGTTTAAAGTTAGAACAACTCAACTAAAAGCCCAAAGAGAAAGGGAATTGAAGAACTTGTTGAATAAAATCAAGCTTAAAAATTTAAGTGAAAAAAAACAAGAAATTTTGTTAAATAACTGGTTTAATAGCAACGAACGTTTGTTTTTAAAAAATGAAGTGAAAAAGGTTAATTGACTAAACTCGCCAAGACAAAAACAACAAGCAGCTCAAATTGATGATCAAAACATTATTGAATTGAAAAATGTGTATAAATACATCACTAATGGCATTACTACAAATGCAGTTCTTAAAGGAGTTGATCTTGCCATTAAAAGTCATGATTTTATTGTGATTTTAGGCCCTTCAGGATCTGGTAAAACCACATTACTAAACATTATTTCAGGGATGGATAGAGCTTCTAGTGGTAGTGTTATTGTCAATGGTTATAACATGATTTGTTTAAATGATAGAAAGCTCACTAAATTCCGTCAAAAGTATGTTGGTTACATCTTTCAACAATACGGTTTATTACCTAATTTAACAGTTAGAGAAAACATTGAGATAGGAGCAAATCTTCAACCAGATCCTAGTAAAAGGATCAGCATTGATGCACTTTTAGAAGCGGTTGGGATGGATAGTTTGCAAAAGAAGCTTCCTAATGAATTGAGTGGTGGGCAACAGCAACGTGTTTCCATTGCAAGAGCTTTTGCTAAAAACCCCTTATTAATTTTTGGTGATGAACCTACTGGGGCACTTGATCTTGAGATGACCCAAATTGTTTTAAAACAGTTTTTAGCAATTAAAAAGCGTTATCAAACGACAATGATTATTGTTACCCACAACAATTTAATTGCTAACTTAGCTGATTTAGTTATCTATGTAGCAGATGGAAAAATAAAATCACTACACAGGAACTTAAATCCTAAACAGGTTGAAGAGATCCATTGAATTTAGATTATGAAATACTTATATGCCACTCAACACCTTACTTTAAATGCTATTAAGCATGCTAAGGGAGGACATGTTGGCATGGCCATTGGTGCAAGTCCTATCTTATTTAGTTTATTTACTAAACACTTTCACTTTGATCCTGACCAACCAAAGTGGATCAACAGAGATCGCTTTGTTTTAAGTGCTGGCCATGGTAGCATGGCATTATATTCAATTTTCCATTTTGCCGGACTTATTTCTAAACAAGAGATCTTACAGCATAAACATGGTCAAATTAACACTTCTTCCCATCCTGAATATGCTCCAAATAACTTCATAGATGCATCAACAGGCCCTTTAGGTCAAGGCTTTGGCATGGCAGTTGGCATGGTGTTAGCACAAAAGTTATTAGCTAATGAATTTAAAGAGCTAAGTGATAAATTGTTTGACCATTACACCTATGTGGTTGTTGGGGATGGAGATCTACAGGAGGGGGTTAGTTATGAAGTTAGTCAAATTGCTGGGTTATATAAATTAAATAAACTAATTGTGCTTCATGATTCAAATAGAGTGCAAATGGATAGTGAAGTAAAAAAAGTTGCTAATGAAAATCTAAAGGTTAGGTTTGAAAACGTTGGTTGGAATTACATCCATACTGATGATCAACTAGAAAATATTGATCAAGCTATTATTAAAGCCAAACAATCAGATAAGCCAACTTTTATTGAAGTGAGAACAACTATTGCTAAAAACACCCACCTTGAAGATCAGTATGGAGGACATTGGTTTATTCCCAATGAAGTGGACTTTCAACTTTTTGAGAAAAGAACAAATACTAACTTTAACTTTTTTAATTATCCAGATAGTATTTACCACTGATTCAAACAAACTGTTATTGAAAGACAAAAACAAATTAAAGAAGATTACAACAATTTGCTAATTTCTCTTAAAGACAAACCACTTTTTAAAAAATTTACTAATTGGATTGACAGTGATTTTCAAGCCCTTTATCTTAACCAACTAGATGAAAAGAAAGTAGCAAAAAAAGATAGTGCTACTAGAAACTATTTAAAAGATTTTTTAAACCAAATTAATAATCCTAATTCCAACTTGTATTGCTTAAATGCTGATGTATCACGTTCTTGTTTTATCAAGATAGGTGATGATAATCTCCATGAAAATCCTTGTTCTAGAAATATCCAAATAGGAATTAGGGAGTTTGCAATGGCAACAATAATGAATGGTATGGCACTTCATGGTGGTATTAAAGTGATGGGTGGTACTTTTTTAGCATTTGCTGATTATTCAAAGCCAGCAATTCGCTTAGGTGCATTAATGAACTTACCAGTATTTTATGTTTATACCCATGACTCTTATCAAGTAGGGGGTGATGGTCCTACTCATCAACCCTATGATCAACTACCAATGTTAAGAGCAATTGAAAATGTTTGTGTATTTCGTCCTTGTGATGAAAAGGAAACTTGTGCTGGATTTAACTATGGTCTTTTAAGTCAAGATCAGACAACTGTTTTGGTTTTAACACGTCAACCCTTAAAATCCATTGATAACACTGATAGTTTAAAAACACTGAAGGGTGGTTATATCCTTTTGGATAGAAAACAACCTGATTTAATTATTGCTGCTAGTGGTAGTGAAGTGCAACTTGCAATAGAGTTTGAAAAAGTTTTAACTAAACAAAATGTAAAGGTAAGAATTCTGTCAGTTCCCAATATAACTTTACTTTTAAAACAAGATGAAAAATATCTAAAGAGTTTATTTGATGCTAACAGTTCACTTATCACCATAGAAGCTAGTAGTAGCTATGAGTGGTTTTGCTTTAAGAAGTATGTTAAAAACCATGCTCATTTAGGAGCTTTTAGTTTTGGTGAATCTGATGATGGAGATAAAGTTTATCAGCAAAAAGGGTTTAATCTGGAAAGGTTAATGAAAATATTTACTTCCCTAAGAAATTAAAATTATCTTAATGTTGTATAGGTTTTGAAAAACAGGATTGGCAATTTTTATGCCTGGTTGCATCTTACTTTCATCCTGTTCTTTTAGAAGTTATATCCCAACTCCTAGTTTAAGAAATACTGTTGGTAATCACAACAGTTATGTTAATAATACTGTCCCTAAAAACAATTTTTATGAAAAGTTTTATGATCTAACTTTTGCTTTAAATTTCACTAATCAGAAAACTCAAGAGTTTGGTACTGGTTGGTTAATTGACTGAAAAGGAGATGAAACTAAAGATCTTAATACATTAACTATTGCTAGTTCTTCTATTATTTCCTCTGTAAGTAATCATTCTTTAAAAGAAAAACAAGATGACAAGCTTTTTATTGCTTATATTGCCACCAATTTACATCTGATAGATGGTTTAAAGAATGATCATGATTATCAACCATACAATAAAGATGGAAATGGTCTTAGTTTTCCATTTGATCAAAAAACCCAATCATTCTTATTGGGTAGGTTTGCCAATCCTAAGATAAATTCCAAACCAGAAGAGATGAACTACCAAGTTCAAACTCGTTTAAAACAAGATGCAATGGTGTTTATCCAAACCAGTACTTTACCTAAAACTGCTTATGCAGGAATTGATCCTATTAACTTTGATTACCATGAAACTAGTGATGAGAGTGGATTTTGAACTAAAAAACAAAGCACTGCAAACTTCCCTAGAACAAGAACATTAAAAAGTTATGCTGATTTTGCAGTTTTAGAGGTACCCTTATTCTTAGATAATGCTAATGATGCCAAGATTTATCAAGAGTGAATTAGACCAGCAGTTCAAGCTTATAAGGAGCTAGGGGATGTTGAAAATATTTTTGCTAAAACCCCATACGCTGAATATATTAATAACACCTACTACTTATTGGGTTATCCTGTTACTAATAACAATAAGTATCAGTTTATCTTAGGTCAAGATGAAAAGTGAAAGTTTTCTCAACAAACTTCTGTATTAAAGCACTATCAAAAACAACCTCTTCAACAAAGAACAGTTTATGTTGAACGTGATGATGGTCTTCCTACATTAACTTTTAATGAAGATAAACTCACTCATGTTCAAGGTACTGATCTAATTAATGTTGATCAGATTACCGATACTAATTTAGGAAATGGCTTAATAAATTATGCTGGTTTATCACGCTTTACTTTAAGCTATCACAATGTTGAATATAAGTTATTTGGTTATGGCACTATTTTAAATAATACTAATTTTCCAGGTGGATCTTCTGGTAGTGCTGTTTTCAACAAGGAAAAACAACTAACAAGTATTTACTTTGGTTCACTGATTAATGTAACAACTGGAAATAACAGGAATGTTAATTTAGGTTTGGGTCAAATTCTTCGTACTTATAACACTAATAATAGTAAGCACAGTGCACCATCATCATATGATTTAATTTTTGGTGATAAAAACACCATCAAGTTTTATGCACAGTTTGCAAAAGAAAAGCAAACTCATCTTTGAAATAAAATTCAAACATCTGTTAACTCTTCAATCAGCTTTTACAAAGACAAAAAATAATAATTAACTAACAATTTGTATTTGTGTTAAGACGTTATCTAACTCTGAGTTTTTCTAGCTTGCTTTTATTAGCACTGCTGTTTTTAACAGGCTGTTCTTTTGTTAGACCACAATTTCGCAGGGGCTTTAGAACCCAGTTTAAAATTAACTCTATTCCAACTGTTAGTGATCCTTATCACATTAACTACGACTTAACTTTTTCATTAAACTTTGCTTCCAACAAAAGAAATACTTATGGTACTGGTTGGTTAATTGATTGGAAAGGAGATGAAAATAACCCTGAGAAAAATGATCCTTTTAAAGTTTATTTAGCTACTAACCTCCATGTGATAGATGCTTTAAGAAATAATAATGACTATGAACCATATAACAAGGATAGTAATAACCAAGCTTTTAATAGTGAAGAGATCACTAGGTTCTTTTCTATAGGTAAATACACATATCCTAGTATTTTTAGTGAATTAAATTTCATTATCAATGCTAGAGAAGCGTTTGTTTCGATTCAAACCTCTACTATTCCTAAAACTGCTTATGCTGCTGTTAACTTTGTTGAAACACAAGGAGAGGATGAAAGTTATACAGATTCATTATCAACTGATAATAAAAGAGATATTTATGCGGATTTTGCTGTGATAGAAATTCCCTTATTCTTAACTAACCATCGCGATTATCAAGTATTTAATGAATTTATTAAACCAGCAATAGAAACATACAAACAACTAGGAAACTCTTCCTTTGAAAAAAAACAACTAGATCAACATAAAAACGACAACTTTTACATGTTAGGTTATCCTTTGGTTGAGAGTAGCATAGATGCTCTAATTTTGAACCAAAGAAGACAGTACAATAACAGTTATACTGAAAAATATACTCCTCAAACTTTAACCAAAGATCAACGTACCATTGACTTAAGCAGAGAAGTTCCTACTTTAATTCAGAACAAAACAGAAAACTCTACAGGAAGTCAATTACTAGTAAATCAATCTTTAAGTAGTACATCTGAAGGGATTATTGAATTTATTAAGTTACCTGAATTTAAACTCAATTATCATAATAAAAGTTACCGTCAATATGGTAGAGGTTTTGCTCTACAAAACACTAACTTTAGACCAGGTTCTTCAGGAACTTTAATGTTAAATAACCAAAAACAGATAGCAGGTATTTATTTTGGTGTTTTAGATTTTGGAGAAGATGTAAGTTTAATGAGTAACATTGGCGTTGGACAAATTCTTCGTGTTCCTCAAAAGAACAATACTAGAAATAGATCAATTGCTACTAATAAAAGCAACTATGATTTGATCTTTGGTGATAGCAATACAACTAACTTTTATGCAAAATTTGCTAGACAAAATAACACCCATCTTTATCAGATGATTTCAAATAGTAAAGATACAAAATTAAAGTATGTGAATACTGTTGAAAAAACAGTAAAAGCTAGTATTAAATAAAACAAAATAGTGACTTTCTTTTTTTATACTCATAAAAATGGAAAATCAAAACAGTTGTAGTTCTGTAAAAGAAAAAGAGTTATATAATCCAAAAAAGCCTTTTCTTTTTGCGGTAATTTCTTTCTTTGTTTTCATAATATTTTTTGCTTTAGCAGGTTCATTTACCAAATTTGCATTAGAAGAAAGCAGGGGTTATAGTTTTCTTGTTATTGTCTGTTATCTTCTAGGAACAGTTTGTTTTTTTGTTTTTATCTATTTTTGGTTTTGATTCTTATCTGAATTTTTTGCAAATAGAGTTCTAGTTAAGTTATGAGATTTACCTACTAAAAAGAGAAGAAAAAGACATGCAAAAAAACAAAGATAATTAGTTAAGTTTTAATAACTCAGTTGGCAAATAAATTAGGGAGTTATTAGAACGCTATTTTTTATTAATTTAAGTGTTTGTTTAAAATTTATTAAGTACTTTTTTAACTTGTTAAAAAAGTAGTTATTTTATTAGTTGGAAAGTGCAAAAAGAATTAAATATTTCAAATGATAAAAAGACTTGTTTAAATGAAATAGAAACAAGCAAAGTTAAATTTCTAACAACAACTTTAATTTTGTTGTGATCAATATTAATAACACTCTTAGTTGTTGTTAGTGTATTAGCATTTAATTTCATTTTATTAGGATCTACTAAACAAGGATTACCAAATACCAATGAAATAGAGACACTAAAAAGTTCATTAGCCTTGCAATTATCACAAAATGGTGTGATCTTATCAATAGCACTACTTGCTTTTTTTAGTTGGATGGCAATTGTTGGAATTCACAGCTTTTTAGTTGGAATTTTAGTTAATCATCAAACACTTAAAATAAGTAAAAAGTATGTTATTTTAGGTTCGATTTTTCCAATTATGGCATTAACAAATACTCTTGTAATTAGAAAAAAATTAAAAGCTTTATTAGGAGAGGGTAAGGTTCAAAAAGGACTCAAAGTTTTAACTATTAGTTTTATTTGTGTTTGAAGCTTGCAATTGATAATAGGCTTTTTTGTATGACTTTTTCCTTATGCTGGTGAGGCAGGAATTAATATAGGAATTAATTTATCTATTTTTAATTTGGCTCAGTTAGTAGGCTCAGATATAAATGTAATAACTTACTTTACAACTTTTTTAACGCTGCTTTTTGCATTTCTTTCATGAGCCGTATTGCATGTTTTAGCTTGCTTTATTTTGGTACATTTGACTATTTATAAACAACAAGAATGAATAAAAATTAAAGCTATTTACATTTTGGCTTTAACCTTAATTGATTTAATGGGTTTAATTTTGGCTTTTGGTATTATTGTTGTTAACAATACAAATTCTTCAAGTGTTGATGCAAAATCAAATTCCTTAGCATTAATTTTTAAGTTCATTTATTTTGGTAGTTCTATAGCAGCCTTTTTAACAGTTTTAAGTTTTGCTAGTTCAGTTGCTTTACTTGTCAATTTAATAAAAAAAGATAAGGGATTAACCTTAAAAAATTAGTTAACCTCCCCCAACCTCTATTTTCTGTTACTAGTGCCTAAGGTGGTATTGGAGTATCACAAGCTCAGTAAGGATGTAGTCAAAGAGAGTTTGGGGGTGGATACATCAGGTTCAACTTTTGATCCAACTAAAAGGTTGAAAAAAGATAGTCCAATGAAGGATTCAAACAAAGACAGTGAGAAACTCACCGAAGCAACTGCTTCATCCATGAGTAGTGGTGGGGCTACATCCACTCGCAAGGCCCTCAAGGTTGAGGTGGAGAAACAAAGTGGATCAACTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCACTAAAGCATAAAGAAAATAGTGGGGAGGTGAAGTTGGATGCGAGTGGGGAGTTTGCCAATGATAAAGCCTGAAAACCGGTGTTGAAAACAGATGAGATAACAAGAGAGAGGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAATGGTTATTCCTTCTCCAACTCCCCCTCCCCTTCTTCTTCTTCTTCTACTACTTCTACTTCCTCTTCTACCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACACTTAAATCAGCAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATCTGAACGGGAGCAGGGTATCGCAAGGGTTCATCAAACACCAACGGCATTCCCTTCGACCAGGTGAAACCTAGCAATAATAGTCAACAGTTTAATCCCAATTCAGATGATAATAAAGTCACTTCAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCGTTGACTTTCACTAATAAGAATAACCCGCAAAGAAACCAACTGTTGCTCAGAGCACTGTTAGGAACGATCCCGGTATTGATCAATAAGAGTGGAGGAAGTGGGAATGAGTTTACCCATACGAGTGAGCAGCAGTGAAACGAAACAGATAAATTAGGAGGTAACCTCCCGGGGTTTGGGGAGGTGAATGGCGGTTTTTACCCAGTTTTACTTATTTAATAGTTAAAAAAGCTTTAGATTTTTATCAAATTTTTATCTATTTAATATTTAAGAAAGTCGCAAATTTTTATCAGTTTATTGGTCAAAGAAGTCGCAAATTTTTCTTAGTTTTTTATTTGCTTAATGGTTAAAAAAGCGTTAGTTTTTACCTTTATTTAATTAATAAACTTCGCCACCCCCATCACCGATTCTAAGGCTGATCTGGTTAGTTTGGCACAACTAGATGATTCCTATCAAATCTCCGACCAAACCATCCATAACACCAACCTGTTTGTGTTGTTCAAGTCCAAGGATGTGAAGCTTACATATAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAGTCAAGCTAACAAACCCGCCTACATCGTTGAATTTACTAATTCCACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAATATAAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACCAAGTGTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAGTATACGCTTAATAGTAGTTTGGCCAAAGAGAAGGGCAAAAGCCAAAGGGAGGTGCATCTGGGTTCAAATTCAAATCAGTGACGATCGATGCGTGACCAACACGGCTTAAACAACAATCCCAGCCCAAATGCTTCAACTGGATTTAAACTCACTACCGGCAACGCATATAGAAAACTAAGTGAGTCCTGACCAATTTATCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACTGAAGCAACAACGGCAAAAAATGATGCGCCCAGTGTTTCTGGAGGGAGATCATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGAAAGGATCGGTATCTTGTTTGAAAGTAATGGAGAGGCGAGGAATGTTGTTAGCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATACCAAACCACCAACACCTACAACAAGTTAATTGAACCTGACAAGTGACAATCAAGTAGTGATTTGAACAATATGACCAACTTGTTAAAACTCCTAACAACTAAAAATGTGAAAGCAAAGTTGGGGAAGACAGCCCAATCTCAGGAAAATAGTGGGGGGGTGAAATGATAAAAATGTTTTTAAATAACTACATTAATTCTCTTAAACTTACTAAATTATTTTTCCATTTCGAACTATTATTTGAAAAAAGTTATTAATTGCAAGTTGATAAAGAAAATGAAGCAAAATAACAAGAAAGAATTATCTAAATTTAAATAGACTAGAATTAAATTAAGACCAAAATGTTGCATGCAAACAACATATGCTCTTTTAGTTGTTTTTGGAATTTTATTAGTTCTAATTGGACTAGGATTTTTATTTTGAGCTTTAAAAATTTCACTTAAAGAACAAAAGAAAGGTCAAATTACTAATGATTTGACTTCCAATAACTCACTTAAAAATGAAGTTCCTAGAGGGAGTTGATGAAAAAGAAACAACAAATTAGTTTTATTTTTGCTTGCAATAGTTTTTTTAATGCTAGGAATAGGTGGACTTGTTTCCCTTCCTAAGCTGTTTGGTTAATAAAATTTAGTAGTTTTAAAATGCAGATTAGTTTAGTTAAAATCCGCAATAAGTTTAAACAAAGAAACCGTGGTTCTTTTCGTCAGTGAGTTGGTAAGCTTTCCAACGGTTTGATGATCCCTATTGCAGTTTTGCCTTTAGCAGGTATTTTTTTAGGAATCGGTGATGCCATTTCTTCCAATTCATCTGGCATTGTTGGTGTGAAATTTTTTGGTGAATTTATTAAACAAGGTGGTAATGTAGTTTTTGCTAACTTACCTATTTTGTTTGCAGTTGCAATTGCGATCACCTTTTCTCAAGATGCAGGGGTTGCTGGATTTTCTGCTTTTGTTTTTTGGGCCACAATGAACGCGTTTATGAGTTCATTAATTATTCCTGTTGATGCAAATAATACTGCTTCAGGTTATAACATCCTTTATTGAAAAGCAGTACCTCAGTCAGCAATTGCTTCTACTTTAGGATTAAATTCACTTTCAACTTCAGTTTTTGGTGGGATTATAGTAGGGGCTTTAACTGCATATTTATATAACAAGTTTTATGCAATTAGATTGCCTGATGTAATTGGGTTTTTTAGTGGTACTAGGTTTGTTCCTATTATTTGTATGACTATTGCTATTCCAGTAGCATTACTTTTATTGATGGTTTGACCTGGTGTTTCTATCTTATTAAATTTAATAGGAACTGGGCTTGGAATCTTAGGTGGAAGAGGATATGGTGCTAACAGTTTAATCTTTGGATATATAGAAAGAGCACTAATTCCTTTTGGAGTACATCATGCCTTTTATGCACCATTATGATATACAAGTGCAGGCGGTAGTTTGCAAGAAATTGCAAATCAACAAGTTTGGATTAGAGCTCCTGGTAGTGATTATGTAACCAGAGTGATAGGTTGAGAAGATTTTAATACTCCAGGAAAATGAGTTATTCCTGCTGCTTTAGCTAATGGAACAAGTGGAATGATGAATGGAGCTACTACAACAGGACAAGATAGTACATCTGCACTTTCAAAATACATGAGTAAAGAATCAACAAACTTTCTAAGTTGAAAAGAACTTGTTGATGGTCTTACACGTAAAGGTAACTTTGATGAATTGGCTAAAAACGGTTTATTAGATGGTTCTAACAAGATTTGAATTGGTTTAAACCAGTCAGGGATCTTAGGTAAAAAAGTACTGTTAAGTGATGGTAAGGACTACACTATTACCTTTAAAACTTTTGCTAACACCACGCCAACATTCTGAAGCCATGGTGCTCATGCACTTTTACCAATTAGTGGAACTCCAAGTGCAATAACTAATGGAGTTACTGTTAATGGTACTGCTAATTCTAAAACCTATAATGTCAGTCAGTTCACTGTTGCAGTTCCTTCTTTAAACCCAGCACAATATTCCCAAGGTAAATTCCCATTCATGCTAATTGGAATTCCAGCAGCTGGACTTGCAATGATCTTAGCTGCTCCTAAGGGTAGAAGAAAAGAAGCTAGTTCTATTATTGGTAGTGCTGCATTCACTAGTTTTCTAACAGGGATCACCGAACCTTTTGAATTTACCTTTCTTTTCTTAGCACCATGGTTATTCTATGGTATCCACGCTGTATTAGCTGCAGTAAGCTTTTGATTAATGAACTTATTGAGTGCTAACGTTGGACAAACCTTCTCAGGTTCTTTCATTGACTTTATCTTGTATGGGGCTTTACCTGATGGTAGGGGTTGATTAGCAAACTCTTACTTAGTACCTATTATTGGTATCTTTTTAGCATTGATTTATTTCCCTACCTTCTATTTCTTGACAATTCGCTTTAACTTAGCAACTCCTGGTAGAGGTGGTAAGTTAATTACTAAAAAGGAATATTTAGCAGCAAAAGCAGCTCAAAAAACTGATCAAACTACTAACACTAACTTTAATCAAACCCAAATTGAAGCTGGTATGTTACTAAGAGCTTATGGTGGAAGTGAAAACATTGCTGAATTAGGGGCTTGCATTACTAAATTAAGAGTAACAGTTAAAAACCCTGAACTTGTTAATGAAACTATTATTAAAGACTTGGGAGCAGCTGGGGTAATGCGTACCACTCCAACATTCTTTGTAGCAGTGTTTGGTACTCGAGCTGCTGTTTATAAATCAGCAATGCAAGATATTATCCAAGGCAAAGTAAATTGAACAGAGTTGCAAAAAGTCTTAGATAAAAATGATAGTACTGTTGAAAAACCAGAAATAAAACCAACCCCAGTTTTAAAAGTTCAAGATGAAATTGTGATCCTCTCACCAGTTAATGGCACCTTAAAACCGCTCACCCAAGTTCCTGATGATACCTTCAAAAATCGTTTGGTAGGAGATGGAATTGCTATCTTACCTAGCGATGGGCACTTCAAAGCACCAGGTGATGTGGGTGTGAAAACTGAACTTGCTTTCCCTACTGGTCATGCCTTTATCTTTGATGTTGATGGTGTGAAAGTAATGCTTCACATTGGGATTGATACAGTAAAAATTAATGCTGATAAAAAACCAGGGGAACAACTTGAAGTGTTTGATGTAAAAACAAAACAAGGAGAATACACTAAATTAAAGAGTGAAAGTGTTGTTGAAGTTGATTTAAAGAAACTTAAACGAAAGTATGATCCAATCACTCCTTTCATTGTGATGCAAGAATCACTTGATAACTTCAAGTTGGTGCCAATTCGCCAACGTGGTGAAATTAAAGTTGGCCAACCTTTATTTAAACTAATTTATAAAGATAAGAAGAGTTAATCAATAAAACTCGATAATAACTAAAAGCCATAAAACCTTGGTTTTGTGGTTTTTAGCTTGTTTATAACAATATTATTGCAGTTTTACTGCATAATGTAAAATTACACAGCATGTCAGATACAAATACTGAAAAACCTGAGTTAGTTTCCCTTAATAAGTTAAGTGAGATGCGCACTAACATCGGGATGGTTAAACGTTATTGAAACCCAAAGATGGGATTCTTTATCGAACCTGAACGTAAGCATAATAACGATTTATTGAAGCTTGATCTACAGTACCAAGCGTTAAAAACTGCTTATAACTTCATTAAGGATGTTGTTAAAAATCACGGACAAATCCTTTTTGTTGGAACAAAGAATGATTATGTTAAAAAACTGGTAATTGATATTGCTAAAAGAGTTAATGTTGCATATATTACCCAGCGCTGATTAGGTGGTACTTTAACTAACTTTAAAACCCTTTCTATCTCAATTAACAAACTCAATAAATTAGTTGAACAGCAAAAGCAAAATGCAAATGATCTAACCAAGAAAGAAAACCTGTTACTTTCAAGAGAGATTGAAAGACTTGAAAAGTTCTTTGGTGGGGTCAAAAATTTAAAAAGACTTCCTAATCTAATAGTTATAGATGATCCTGTTTATGAAAAAAATGCAGTTTTAGAAGCAAACAGCTTAAAAATCCCTGTTGTGGCACTATGCAACACCAACACCAATCCAGAGCTAGTTGACTTTATTATTCCAGCTAATAACCACCAACCCCAAAGTACTTGTTTATTGATGAATTTACTAGCAGATGCGATAGCAGAAGCGAAGGGTTTTGAAACCTTGTATGCTTACAAACCAGATGAACAGATCCAAATTGAAATTCCTCCCAAACAAGAACGCCAAGTTATTAACCGTTCCAATACCAGAAACATCACTAACCAGCGCTTAAACATTAACCGTCAACAACAAGAAACTTTATAGAGCAGTGAACAGTTGAACAGGACTTAGTGAACAAGCGGCAATTAAAAGTCGTCAAGAACATGGTGCTAATTTTCTTCCTGAGAAAAAAGCTACCCCTTTTTGGTTGTTATTTCTTCAACAATTTAAAAGTTTAGTTGTTATTCTTTTACTGCTAGCTAGCTTGTTATCGTTTGTAGTTGCTATTGTCAGTGGTTTGAGAAGTAACTGAAACTTTAACCATGATCTGATTATTGAATGGGTTCAACCTTTTATTATCTTATTAACTGTTTTTGCCAATTCACTAATTGGTTCTATCCAGGAATTTAAAGCCCAGAAATCTGCTAGTGCTTTAAAGTCCTTGACAAAGTCTTTCACAAGGGTTTTTAGGAATGGTGAATTAATTAGCATTAATGTTAGTGAAGTTGTTGTAGGAGATATTATTTTTGTTGATGCAGGAGATATTATCCCTGCTGATGGCAAATTACTACAGGTTAATAACTTACGTTGTTTGGAAAGCTTTTTAACTGGTGAATCAACTCCAGTTGATAAGACTATTGATAGCAATGAAAAAGCTACTATTCTTGAACAGACAAACTTAGTTTTTTCAGGGGCACAAGTAGTTTATGGTAGTGGCGTTTTTCAAGTGGAAGCAGTTGGGATTAAAACCCAAGTTGGAAAAATTGCTAAAACTGTTGATGATAGTGTAACTAAACTCTCACCCTTACAACAAAAACTAGAGAAGATAGGAAAGTGATTTAGTTGGTTTGGGCTTGGTCTTTTTGCTGTAGTTTTTCTTGTCCAAACTGCTTTATTAGGATTTGATAATTTCACTAATAACTGATCAATAGCTTTAATTGGTGCTATTGCGCTTGTTGTTGCAATTATCCCTGAAGGGCTTGTTACTTTTATTAATGTGATCTTTGCATTAAGTGTGCAGAAACTAACTAAGCAAAAAGCCATTATTAAGTATTTATCAGTAATTGAAACACTTGGATCAGTACAAATTATCTGTACTGATAAAACTGGTACTTTAACCCAAAACCAGATGAAAGTTGTCGATCACTTCTGTTTTAATTCAACAACCCAAACTGATCTAGCAAGAGCATTGTGCTTGTGTAATAATGCTTCTATTTCCAAAGATGCTAATAAAACAGGTGATCCTACTGAAATTGCTCTCTTGGAATGAAAAGATCGCAGTCAATTAGATTTAAAAACCTATTACAGGGTTTATGAAAAAGCCTTTGATTCAATCAGAAAACTTATGACAGTTGTTGTTCAAAAAGACAACCGCTTCATTGTGATTGTTAAAGGTGCTCCTGATGTGTTATTACCATTATGTAATAACGTTCAAAATGAAGTAAAGAACATTGAAAACTTACTTGATCAAAGTGCTGGTCAAGGCTTGCGTACCTTAGCAGTTGCTTTAAAGGTTTTATATAAGTTTGATCAAAACGATCAGAAGCAAATTGATGAACTTGAAAACAACCTTGAATTCCTTGGGTTTGTTAGTTTACAAGACCCACCAAGAAAAGAAAGTAAGGAAGCGATTTTAGCGTGCAAGAAAGCTAATATAACCCCAATAATGATTACAGGGGATCATCTTAAAACTGCAACTGTAATTGCTAAAGAGTTAGGCATTTTAACTTTAGATAATCAAGCAGTTTTAGGTAGCGAACTAGATGAAAAGAAGATCTTGGATTACAGGGTATTTGCTAGAGTAACTCCCCAACAAAAATTAGCCATTGTTAGTGCTTGAAAAGAAGCGGGATTTACAGTTAGTGTTACTGGTGATGGGGTGAATGACGCACCTGCATTAATCAAGAGTGATGTAGGGTGTTGTATGGGGATTACTGGGGTTGATATTGCAAAAGATGCTAGTGATCTGATTATTAGTGATGATAATTTCGCTACTATAGTAAATGGTATTGAGGAGGGTAGAAAAACTTTTTTAACTTGTAAACGAGTTTTATTAAACCTGTTTTTAACTTCAATTGCAGGAACAGTTGTAGTTTTATTAGGACTATTCATCTTAGGACAAGTTTTTAAAACTAATTTATTACAACAAGGTCATGACTTTCAGGTGTTTAGTCCTACCCAACTGCTAATTATTAACTTGTTTGTTCATGGTTTTCCTGCTGTTGCATTAGCAGTACAACCTGTTAAAGAAAAATTGATGGTAGGTAGTTTTTCTACTAAAAATCTGTTTTACAACCGCCAGGGATTTGATTTAATCTGACAATCACTATTCTTAAGCTTTTTAACTTTATTGTTCTATAGCTTAGGAATTATATATGCAATTAATAACCGTGATTTACAAACTAGCGGGGATCTAATTAATCGTGCTGGATCAACGTGCGGTTTTTTTATTTTGGGTGCTAGTGCTGCTTTAAACTCATTAAACCTAATGGTAGATAAACCATTGCTTATGACAAACCCTTGGTTTTTTAAGTTAGTTTGAATAGGTTCACTTGCTTCTATACTGGTATTTTTATTGATCATCTTTATCAACCCTTTAGGGTTAGTGTTTAATGTCTTGCAAGATTTAACTAATCACCCAGTTTTAATAAGCTATAGTTTTGGGGGAGTTATTTTGTATATGGGGATGAATGAAGTTGTTAAACTTATTAGATTAGGTTATGGCAATATTTAACTTCCTTAAGTTAATTTCACCCAAAAACAGAATTCTCAGTAAGGCAAATAGGATTGCCAGTGAGGTTGAGAGTTATAAAAACTACTACCGTAACTTAACTGATCAACAGTTATTTGAAGAGTCAAATAAACTAGTTGATCTTGTCACTAAGCAAAATTACACCATTCTAGATGTTTGTGTTGCTGCACTTGCTTTAATTAGAGAAGTGGTTTACCGTGAGACTGGTGAATTTGCATATAGGGTGCAGATCATAGGAGCTTTTATTGTTTTAAGTGGTGATTTTGCTGAGATGATGACTGGTGAAGGTAAGACCTTAACCATTGTTTTAGCAGCATACGTTTCTGCACTTGAAAAGCGTGGTGTGCATGTTGTTACTGTTAATGAATATCTAGCTCAAAGGGATGCTAATAATGCAATGAAGATCTTAAAACGGGTTGGGATGAGTGTCGGTTGTAACTTTGCTAATCTCTCCCCTCAGCTAAAACAAGCTGCATTTAATTGCGATGTTACCTACACCACTAACAGTGAACTGGGGTTTGATTATCTTAGAGATAACATGGTCCACAGTTATCAAGATAAGAAGATCAGAGAGTTGCACTTTGCAATAGTTGATGAAGGTGATTCAGTTTTAATTGATGAGGCGCGAACGCCTTTAATTATTTCAGGTCCTAGTAAAAATGAGTTTGGGTTATATGTTGCAGTTGATCGATTTGTTAAATCATTAACTGAACAGGAGTTTAAGATTGACCCTGAATCACGTGCTGCTTCTTTAACTGAACTTGGGATTAAAAAAGCAGAGCAAACATTTAAAAAAGAAAACCTTTTTGCTTTGGAAAACAGTGATCTTTTTCACAAGATCATGAATGGTTTGACTGCTGTGAAAGTTTTTGAACAGGGCAAAGAGTACATTGTTCGTGATGGCAAGGTTTTAATTGTTGATCACTTTACAGGTAGGATATTGGAAGGGAGAAGTTACAGTAATGGCTTACAACAAGCTGTACAAGCCAAAGAATATGTTGAGATAGAACCTGAAAATGTGATAGTAGCTACCATTACCTACCAATCCTTCTTTAGGCTATACAACCGCTTAGCAGCAGTATCAGGTACTGCTTTAACTGAATCAGAGGAGTTTCTCAAGATTTATAACATGGTTGTAGTACCAGTGCCAACTAACCGTCCTAACATCAGAAAAGACCGTTCTGATAGTGTATTTGGTACCCCACAAATTAAGTGAATGGCAGTTGTTAAAGAGATAAAAAAGATCCATGAAACTTCTCGACCTATTCTGATTGGAACTGCTAACATAGATGATTCTGAACTCTTACATAATCTGTTACTAGAAGCTAATATTCCCCATGAGGTTTTAAATGCTAAAAACCATTCAAGAGAAGCGGAGATAGTAACTAAAGCAGGACAGAAGAATGCAGTTACTATTTCAACTAACATGGCTGGAAGAGGAACTGATATCCGTTTAGGTGAAGGGGTTGCTGAAATGGGTGGTCTTTATGTATTGGGAACTGAAAGAAATGAGTCAAGAAGGATTGATAACCAACTAAGAGGGAGAGCTGCTAGACAAGGTGATAAAGGGGAAACTAAGTTCTTTATCTCACTAGGTGATTCATTGTTTAAACGTTTTGCTCATGACAAGATTGAAAGAGCGATTAGCAAATTAGGTAATGAAACATTTGACAGTGCCTTCTTTTCCAAAATGTTAAGTAGAACCCAAAAACGGGTGGAAGCAATTAACTTTGACACTAGAAAAAACCTGATTGATTATGACCATGTTCTTGCAAGTCAAAGGGAATTGATTTACAAACAACGTGATAAGTTTTTATTAGCAAACGATTTAAGTGAAATGATCGACAAAATGCTAGAAAAGTTTGTACAACAGTTTTGTGATCAATATAGAAACCAAAAGAACCAAAACTTAATTAATCACATTGCACTAGCAGAAGCTTTAAATCTTGAGATGAACATGCAAAACACCATTAATCCAAAGGTGTTTGAAAACATGACTTTTGATGTTGCTGTTGATAAAACCCGTAACTTAGTAGCTAAAAAGATTAGTGATAAAGTTAATGTTTTGACCAAACCAATTGCTTTAAACAGGTTTCGTGACATTATCATAACTTCGATGGATAAACATTGAACTGAACACTTGGATAGTGTTTTTAAGTTAAGAGAAGGGGTTGTACTTCGTTCTATGGAACATACGAGTCCTTTAAATGTTTACATTAAAGAAACAGATATCCTTTTTAAAACAATGTTGCAAAAGATTGCTCAAGATGTCATTGTGCAAATTGCTAACCTCACAACTCCAGATGAATTTGATCATAGCTTAATGCAAGCCAATGCTTTAAAGAAACTAGCAGCAATTAAAGCAGATGAAAAATCAAACCAAGAGTAATAGTTTATTTCAACTTTCCACTAACTATATACCTACTGGTGATCAACCTGAAGCAATTAAGAAATTATCAGAATTTAAAACTAAGCAGCAGGTTTTATTGGGGGCCACAGGCACAGGTAAAACCTTTACAATTGCTAATGTAATTCAAAACAGCCAACTCCCAACAGTTGTTATTGCTCATAACAAAACCCTAGCAGGTCAACTCTTCAATGAATTAAAGCAACTGTTTCCTAAAAATGCAGTTGAATATTTTATCTCTTACTTTGATTTTTATCAACCTGAAGCTTACTTACCCAGTAAAGGGATCTACATTGAAAAAAGTGCTACAGTCAATGAAGCGATTAAACGCTTAAGAGTCTCAACACTGCATTCACTTTCAACAAGAAAAGATGTTATTGTAGTAGGTTCTGTTGCTAGTATTTATCCCACCTCATCTCCCAGTGATTTTGTTAAGTATTGCTTGTGGTTTGTGGTTGGCAAAGATTATGATTTGAAAACCATTAAAGATAGGTTAGTTAGTCTTAACTATGTTGTTAATAAACAACAATTAACCCCAGGAAAATTTCGCTTTCAGGGTGATGTTTTGGAGGTATTTCCTGGTTACAGTGATGCTTTTGTGATCAGAATCTCCTTTTTTGATACTAAAGTAGAACAAATTTGTCAAATTGACCCACTAACAAATAAGATTTTAAACCAACTCTTTGAGATTAAGATAGGTCCTGCTGATGAATATGTTGTAAACCAATCTGATCTTGATATAGCAATTAAAAATATTAAACAAGAACTTCAGGAACGAGTTAATTATTTCAATAAGCAAAATCTTGTTGAAAGAGCACAACGTTTAGCCACCATTACTAACCATGATCTCAATGATCTGAAGGCTTGGGGATTTTGTAGTGGAGTTGAAAACTATGCTAGACACTTAGAGTTGAGGATGGCTAACTCAACCCCTTACAGTATCTTTGATTATTTTAAGGGGGATTGGTTACTGGTTATTGATGAATCACACCAAACTTTACCGCAACTTAATGGGATGTATAACACTGATCTTTCAAGAAAGCAAAGCTTAATTGATTATGGTTTTCGACTCCCCTCTGCACTTGATAACAGACCGCTCTCATTTGCTGAATTACAACAAAAAATGCAAAAAGTTATTTATGTTTCAGCAACTCCAAGAGATAAAGAGATTAGTTTAAGTCAGAATAATGTCATTGAACAGTTAGTTAGACCAACTTACTTGGTTGATCCTATTATCGTTGTTAAACCAAAAGATAACCAGGTGGAGGATCTCATTGAAGAGATTATCAACCAACGCCAAAACAACACAAGAACATTTGTTACTGTTTTAACTATTAAGATGGCTGAAAACCTCACTGAATACTTAAAGGAACGCAAAATTAAAGTTGCCTATATCCATAAGGACATTAAAGCATTGGAACGTTTATTGTTAATTAATGACCTGAGAAGAGGTGAATATGAGTGTTTAGTTGGGATTAACCTTTTAAGAGAAGGGTTAGATGTCCCTGAAGTTGCTTTAGTTTGTATCTTTGATGCAGATATCCCAGGACTACCTAGGGATGAGAGAAGTTTAATCCAGATTATTGGACGTGCTGCTAGAAATGAACATGGTCGAGTTGTTATGTATGCTAACCATGTTACTGAACAGATGCAAAAAGCCATTGATGAAACCAAAAGAAGAAGAACTGTTCAAATGGAATATAACAAGCTACATAATAAGACACCAAAAACAGTTGTTAAACCCCTTACCTTTGTTCAACCAATCAAATTAAAAGCTAAGAGTAATGCAGAAAAAAATGCTGCATTAATCAAACAATTAACCAAAGAAATGAAGAAAGCAGCAGCTAATCAAAATTATGAACTTGCCATTGAGATTAGAGATTCCATATTTGAATTGGAAAAAGAAATTGGTAGTAAAATTAAAGTATAGCAATGCGACAGTTTATTAAGCTTAGCCTCTTAGTCTTTGTCTTGCTTTTCTTAAGTGAACTTATCTGTAGGTTCTCTTTACGCTTAGTAAATTCTATTAAAGCGAGATATAAATCTTCAGTATTTTCCTACACTGCTTGTTTGCTATTTTTAAAGAGTTTTCAAAACTTTTCTAATGCTTTTCAGAAGTTAGCTAACTGGGTATTTTGATTTGAAAATGATGTTAATGAACTGTTATCTATCTTTTACTTTAACTTTGATCAGAAGAGTGAAAAAGTAGATTACAACTTCTTTAATGGTTATAAAGTTACTGCTCAAAAAGTAGTTGAAAAAGAACAGTTATTAACTTGTAAGTTAAGTGATTACTACCGTTTATTTAGAGATAAAACCTTTTGATTTGAACTTATCAACAACTAATAAGATCCTTATTGGCTGTTTAAGTTTGCTCTCTTTATAAATTCATAACTTTTCTCTTAACATATGAAATTAAGTACAATAACTACTATCTGCTTGTCAATATCTGGTGCTTTTGGTACTACTGCAATTGCTTTACCAACTACAGTAGCATTGTTAAAAAACCACCAACAGCAAAACACTGAAAAACAACAAAACCCGATTAAAGATATCCGCTTTGGTTTAAACAATGTACAGGTTCCAAATACCATTCCGTTACACCAAACTGTGGTTGAAGTGACCAACAACAAAGCAATTGTTGATTACAAAGATGCACCCCAAAAATTCTTTTTAGCTAAAAGTGCGTTAAATAATAAACTCCAAGTTGAGTTTGATAAGTTCTTGTTAAGAACAGGAGTGATTAATGCTTTAAATGCAGATTTGAAAGAATGGATAGACCAAACATTATTTATCCCCAATCAAAGTTTTTTTGATCTTAGTGCTAATAAACTCAATTTAACTTTGTCAAATCAAAGCGAAGTGAGTTTAGATCTTGAGTTTATTTTTACTAACTTTAGTGATAAAAATCAACCATTAAAACTCCCCTTTGATGGTAGTGTGGTGGTTAATGCTAACGAGTCATACACCTATTCAGTTAAAGCAACACTACAGAAACTAAAAGTATTGACTTATTCAAGAGCAGATCATTCTGTAGGGATTAGTTATGCAATACCAACAGTGAGTTTAAATGGCAAAACTCAAAATGACTTTAGTTTTAACCCCTTTAAAAGTAATATTAACTTTGCTTTCAAAAATGTCTACAATGCTTTAAATCCGTTTGAAGCACAACAATACCTGGTTGGCCAAGGTAAGTTTTTAAACCAAAAAGTTAATGCTGATGATGTTAAAAATGACATTAATAATCACATTGAAACCCAGTTTAATGTTGCTAAAATCACAGCAACTCTTTTAGGAAAAGCTTTTAAGCAGTTTGGTGAACATAAAAATGGTCAACCACTTTCACTTTTAAAAGTACTAAGTGGATTAAACAATGAGTTTAAACAACTCTTTAATTATGTCAGACCTGGTTTAGGTGATTTTGTTAGTGATTTAATCCAAAGCTCCAGTCAATCAAGTAATAAAAAAACTGTTTACCAGCTATTATTTGAAAACAAGACAACAATTATCCACTTGTTGCAAGATCTTAATATTAGTGAACTGAACTCTGTTTTACCAGTTGTTGATATTTTGTTTGAAGGGATTAACAGTGCAGAATCACTATACCAAAGGATCCAATCATTTAAGGATTTAATTGTTCCTGCTTTAAAAGCTGATAAGCAACTTAAGAGCTTAGAAGCGATTATTTTAGCAGTTTTAGACAATCCTAATACCTATGTTTTTGATCTTGTTTACCAAAACAAATCGATTCTGTTTAATTTGTTAAGTGATTTTCTTAAAAACACCGCAAACACTTTACCATTTCTTCAAGAACAGTTTGATATTGTTAACCACTTATTTGCCAATGAAGCGATCTTTGATCTGTTTTCCAATGCTGACTTTGTTGAGAAGATTGCTGATCTTTTTTTAGCAAAACAAAAGGTACAAGAAGTTAATAATGATGGAACTAAATCAACAAAAATTGTTGATTCGATTTTAGTTGCTACCCTTAAGGGTTTAGTTGGTGATCAGCTCAGTTCAATCACTGAACTGTTAAACATCTATATCTTTGAAAATGAATTTTTAAACAGAAATGATAGTAATAGTTCAGTTAAAAAACAACAAACTGATAGTCTAAAAAATCTGTTTAGCGTAATTGGTGATATTTTAAGTGAGACCAATGTTAACAAGATCACTTTACACGCAGTTAAAAATAATGAACTTCTTTCGTTAGTTGAAACAGCTTCAACACTAAAAATTAAACATCTTAATGTTCAATACAAAGTTTTAGTTGATAAGTTTGAATTGAAAAACAGTTTCATTAAGGAACTATTAAATTTTTTCCCAGATACTAAAGATATCACCCCAACTATTAAAAAGGTACTGTTTGAAAGTGAAAATTATAAAACACTCCGTAAGAAGTATGAAAATGAAGGGTTCCCAGGTTATCACTGGGCTAAGTTCATTGTCCCAGGTACCTTCAATTCTGCTGAAAACACTTTCTATTCTGCTATTGATAAAACTAAATCAATTCGCGATCTGTTTGCTGACATGTTATTTGGTAAGAGTTTAGAGAGTGTTAATGACAGTGATAGTTTTATCAAAATTAATGGTTCATTTACATTAAAGTACCACGGTGATAACTTAAATTTACTCCCTAACTACCACTCATTAATCACTAAGAATGTTGGTTATCAAATAGTTAATGTTAATTTTCATATTGATGCAAGATTGCTAACTGCAGAACTACAAAACACGGTATTTTCAAATCCAAAACCAGTTATTAAATCACCTGTTGAACTCTCCAAATCCTTATTTGAAGTTTGAAAGACTATCTTTGAAAACTCAGTTAACCAAATCTTAAAAAAAGAATATACTTTCAAAGATAATCTAAAGTTCTTTCCTTTTAAAGCTGATGGCTCCTCGCGCTTAGAATTTGATCTATCAAAACCTGATCAACGTGTTATTCCTTTTGCTTTTGTTGATGGTTATCAATTCCAACTAAAAAAAGAGTTAATTCCTAACAAAGAAACAAAAAAAGAAGCTAATTCTTCACCAGTTTTAAAGCTTTATGATGCAGTTAAAAGAAACGATAGACAATATCGTCCTAATCACCACCATGATGATCTGAGAAACTATCCTAGCCTTAAATCTCAACTAGAACTTATATTAAACCTTGGTGATAAGCTTAAAGCTAATAATGATTTTATAGATGACACTGTTGTTAATGCTTTGCAATATAAAACTAGTTTTAAATCTACTCTAAAAGTTAACAGTTTAGGAATTCCTATTAACCTTTTCTTTTTCACATTGTGACTTAAATTTAACTTAGAAATTCCAATTGACGGTTCATTAACATTAACATCCGTTAATGTTGTTTTCCCATACAGTTTGTATGATACTAGTTCAAATGAATTTACAAGGATAGTCGATCGCTTAAATTTCACTGATACCAACTTCTATCTAAAAGATGCATTTCCTAATTTCTGGTTTGTTGGTTTCTAGAGTAATGAAACTAAACCACTAACTAAACTACCAACTAGATAAACAATCAAGTTCATCCACCAAAACCCACTAGTTTTTTGTCTGTAAGTTGCACTTTGATCTTGATATTGATTAAAACTAACTAATGGTTTTGCATAAACACTACCTAACTTAAGATATCTGTTAAACTTACTAATTTCATACCGCATTCCATCACAAGTTTTTAAACGGAATATCCCCTGTAAACAAACAAGTAATAAGTTAACAAAAAAGCTAGTTAGTACTGAATTTTTAACAACCACTGTAAAATTGGTTGACTTGTGAAAATAAATAGTAATGTTTAAGATCAAAAAGAAAAGGATTAGATAAAATACGCTAACAACTATCCCACCATACTCAGCTTTATTACTGTTTTTGTTTTGATTTAAAACCATCTAGTAATCGTTATTAGTATCATTTTTTTATAAAATAATCAAGAACATCAGCACATGATCTTTATCACTAAATTTATGTTTGGTTTCAAATGAAGTTGGATTATAAATCCTGCTTTATTGTAATCATGCTTTAGATTTAGAAGCTGGTTACTTTTGTTTCAGGTAACCAGTACCATTTCCAAAAAACTGAGAACCTGAAAAAAACAGGTTCTTTTTTTATTTCTATTTAGTAAATGTTCAAGTATATTCTTAAACGATTAGGACTAGCAGTAGTTGCGATGTTTATCGTAATGTCTATAGTCTTCTTTTTAGTGAACGCTACTGGTAATGTTCCCTTGTCAGCCACTTCTGCAAGAGATATTGCTGCAGTGCAAGCACAACTACAAGAGTTTGGGTTTAATGACCCTATTATAGTTAGGTATTTTCGCTATTGAGCTAAGCTATTTTCCTTTCAAGCTGATGCTTTAGGAATTTATTATGCAAACCCTAACCAAACAATTGGTGAGATTGTGTTTGCAAGAGTACCAAATACCTTATATGTGGTTTTAATCTCTTTTTTAATTGGTTCATTGCTAGGGATCTTTTTAGGGATGGTTTCAGGATTGAATAGAGGGAAGTTTTTAGATGCAGCAATTAATGTGTTGGTAGTTTTATTTGTATCTATTCCTTCATTTGTAGTGGGATTAGGGTTACTTAAACTAGCAGGATTTTTAAATCTACCACCACGGTTTATTAACTTTGATGATGCTTTTTTTAGCTTTGATCGTTTCTTGCTTGCATCAATTATCCCGATCCTTTCATTGGTCTTCTATTCATCAGCTGCTTTTACATACAGGATTAGAAATGAGGTGGTGGAAGTGATGAATCAAGACTATATTAAAACTGCAAAAAGTAAGGGACTTGGGATGTTTGCTGTAGCTAGGTATCATATCTTTAGAAACTCGATTATTCCTTCTATTCCCTTGTTTGTATTTGGAATCTCAGGTGCTTTTTCAGGTGGATTTATTATTGAGTCTTTGTTTGGAGTACAAGGGGTATCTAGGATCTTAATTGATTCAGTGCAAGTTAATGAAACTAACATGGTAATGTTTAATATCTTGTTTATCCAAGGGATTCCCTTATTAGCAAGTGTCTTTATTGAATTTATCTATGTTTTAGTTGATCCTAGAATTAGGATTGCAAATAGTTCTAATGTTAGCTTATTAACTAAGTTAAAGTTCTTAAGTTCAAGACACCAATGGTTAATGAAGTGAAACAAGATTAACAGTGATAATGCCCAAAATATTGTGTTTAACTCGCCACTGCACCACCAGCTATTAGAACTCAATGCAATTGATTACAAAACAAAAACAGTTCAACTAACAACTGAACAAAAAACTGCTCTCAATATCAGTGCAACTGCTAACTTTATCTTACTTGGTAACAAGTGTTTAAAACTCAAAACAATCCATGGATAGAAATAAAAGTTTTGACCCTAACTTATTTAAAAGGGTTGATATCAACTTATTAAAGCGAAATGATCAGCTTATTGGTAAACCAACTACCAATTCAATAGAAATTATCAAGCGCTTGTTTCAAAACAAGTGGGCCATCTTATTTTTTCTTTTAATAGTTGTTATTGTGCTATTAGCAATTATTGTGCCTTTAACTTCCCCTTTTTCAGCAGTAACTCCTGTTTCAACCAATGCCTTAGCACAAAATCTACCACCACGGTACTTATGGCATAAACCAGGTGACATTTTAGTTCATAAGATTACAGCAAGATCAATTGCTGAAATCTCTCAAGCTAGTGGAGTTTTAGTAGGAACATTACCTAGTGCAAATAGTAATCCCTTAGCAACTAATGTCCAGTATGATATTGCTCCTTTTCAACTCCAAGAATTGCGTAATTATTTCCCTTTATTGGGGACTAATGGACTTGGGATTGATATTTGAACCTTGTTGTGAGCTTCTGTTGCCAAGTCATTGTGAATTGCAGTAGTAGTAGCAATTATAGCAATGGTGTTTGGAACCATTTATGGAGCGGTTGCTGGAAGCTTTGTTGGACATATGGCTGATAACATTATGAGTAGGATCATTGAGATTATTGATATAGTCCCTTCTATTCTTTGAATTATTGTCTTAGGAGCTACATTCCGCTTTGGTGGGGTTAAACAATTTGATGATAGTGTTGTAATCTTTACTTTAATCTTTGTGTTTTGAACATGACCTGCTACTACAACCAGAATTTACATTTTGAAAAACAAAGATACAGAGTACATCCAAGCAGCTAAGACCCTAGGGGCACACCAAATCAGAATTATCTTTGTTCATATGTTACCTGTTGTATTTGGGAGATTAGCTGTTGTGTTTGTTAGTTTAATCCCAGCAGTTATTGGTTATGAAGCTTCCTTAGTTTTCCTTGGGTTAAAACCAGCTACTGATATTGGCTTAGGGGCACTTTTAAACCAAGTAACTTCAAGTGATAATGTAGCTTTAATCTTAAGTTCGATTGTTAGCTTTGCAGTTTTAACAGTAGCAGCTAGAACATTTGCTAATGCTTTAAATGATGCAATTGACCCTAGGGTTGTAAAACGATAAAATGGCACTTAAAAGAAGTAATTTCTTTGTTGATAAAGACCAACAACTAAAGGATAATTTGATCTTAGACATCACTGATTTACATGTTAACTTTAAGGTTAAAGATGGGATCTTACATGCTGTTAGAGGGATTGATCTTAAGGTAGAGAGGGGTAGTATTGTAGGGATTGTAGGTGAATCAGGCAGCGGTAAATCAGTGAGTGTTAAATCAATTATTGGTTTTAATGACAATGCACAAACTAAAGCCAAACTGATGAACTTTAAAAACGTTGATATTACCAAACTAAAGAAACACCAGTGGAAGTATTATAGAGGGACATATGTCTCTTATATTTCCCAAGACCCATTGTTTTCTCTAAACCCAACAATGACGATAGGAAAACAAGTAAAAGAAGCGATTTATGTGGCTTCAAAAAGAAGGTATTTCCAAGCTAAATCAGACTTAAAATTTGCTTTATCAAATAAGGAGATTGACAAAAAAACTTATAAAAGTAAACTAAAAGAGATCAAACAAACCTACCAACAAAAAATAAAACCTATCAATGTAGAGAAAAAAACCTTAGAGATCCTGCAGTTCATTGGTATTAATGATGCCAAGAAACGTTTAAAGGCATTCCCAAGTGAGTTTTCAGGAGGGATGAGACAGAGAATTGTGATTGCTATTGCAGTAGCAACTGAACCTGATTTAATTATTGCTGATGAACCTACTACTGCACTTGATGTAACTATTCAAGCTAAGGTATTAACTTTAATTAAACAACTCCGTGATCTACTTAATATCACTATTATCTTTATTAGTCACAATATCTCTTTAATTGCTAATTTCTGTGACTTTGTTTATGTTATGTATGCAGGGAAAATTGTAGAACAGGGTCTGGTTGAAGAGATCTTTACAAATCCACTCCATCCCTATACATGGGCATTGATTTCTTCAATTCCTGAACAGAAAGATAAAAACAAACCACTAACTTCTATCCCTGGAGTTATTCCTAACATGTTAACCCCACCAAAGGGTGATGCTTTCGCTAGTAGAAACCAATATGCTCTAGCAATTGACTTTGAATACCATCCACCCTTTTTTGAAGTTACTAAAACCCATAAAGCAGCAACTTGATTGCTGCATCCCCAAGCCCCTAAAGTTGAACCACCTCAAGCGGTTATTGATAACATTACCTTAACCAAAAAAGCACTGCAATTTAAAGATCAATAATGGAAAACCAAAACACAAAAAAACCACTTGTTAATGTTAAGGCTTTGAGCATGATGTTCAAGGTCAGAGGAACTCTTTTTAAAGCCCTTGATGAAATTGGTTTTACTGTTAATGAAGGGGACTTCTTTGGGGTTATTGGTGAGAGTGGTAGTGGTAAATCAACCACGGGAAAATGTTTGATTAGATTAAACATTCCTAGTGGTGGAAAGATTGAGATTGCCAACCACTTACTCTCAGGAAAAAAACTTACTAAAGAGAATAACCAGTGGTTAAAACAAAACATCCAAATGGTGTTTCAAGACCCTTATTCATCTATTAACCCTACTAAAAATGTGCTAACTGTGATTTCAGAACCGCTGGTAATTAGTAAAACTGTTTTTGGGGAAACAAAACAATACTTAAAGAGTTTGCAAAAGCTCTCTTTTAAAGTAAAGAAAACATTGTTAAGGAATGATATTGAACTTGAAACCAAGTTTCACAATAACTTTTTTAAAACCGTTATTAAGCAAATTAATGAATCATTGTTTAACTTTGAAGATCTTGATTACAAGGATTTAAAACCATCACATTTAAGGCAAAGAATCATAAATGAAACAGATAAATTCATTGAAAAAATTAGAAGTGAGTTTGCCCTTTTTTATGATTTTTATGCTAACCAATCAGTACCCTTGCAAAAGGCATTAGATGATGCGAATTCCTCTTTAACACCATCTAGTGTTATTGAGTTAAAAAACCAGTTAAAAGCATTACAAAAACAAGCAAAGATTTCAAAGGCAGCATGGGATATTTTACAAGCCCTAAAGCAAAACCAAAAGGAGTTGAAAGATTATGAAAATTATGTCCATTTTGAACTCCAAAAAAAGCCACGAATCTATCTTAATACCTGACTTTTAACAACCAAAAGCTACATTAAAGATTCCAAGCAAAACATGCAGCTTACTGATGATATCTTTGCTTTTTCATATAACAGTATGGTTGACAAGAAAAGAAACTTGGTTTTAATTCTTTCTAAATACTATAAGCTGTTACCTTATTTCTATGACCAATCAGTATTTGATAATGCTGATCAATTTGATGAAATTGCTAACCTTATCTTTTTTGATTTAGTTGAAACATTGCTTGGTGTAACTAGTTTATTTAATGATGCATTAGCAGCTGATAAAGTCCCACTAATTAAGTTTGCTAAGTTCTTAAATAAGTTATGTGACTTGCGCTTTTTAACCTTAAAAAAGAGCTTTAAAAAAACAAGAGTAAGTTGTAGCTTTAGTTTTAACAGTGAACCTGAAATCTTGTTTGCCAACAGCTGCTATGATTTGCAACAAATGCCTCAAATCATTAAACCCTTTTGAGAGAAGCTTTTTAATGAACAGAACTACCAAAAGATTATTGATTCAGTTTCAAGACTGAATGTAATGATTGCAAATTACATTACCAAAGCTTTTGAAATTAAAAAAACTATTGATGAAAAACTAAGGGAGTTTAAACAACAAAATTTAGCTTTAAAAAAAGCTTATTCAGCTAACAAGAAAAGTGAGGCAAACAAAGCTTCCATTAATGAGTTAAAAGTCAATTTAAAAACACTTAAAAAACAGCTTAAACAAGAGAAAAATACTACTAAAAAACAATCAAAAAAGGAATTAAAACCACTTTTAAAAGAACACCATACTGCTTTAAAACTCCATGATGAGTTTAACCATGATTTACGCAAGTGGTTCAAAAAACTTAACTTTATGGTTAAGAAATACAACCGACTGGAAAACAGCCAGAAAAAGTTTTGTTTAGTTAAAAAGTTAAAAGCGCTTTTCAAAAAACAGGATGAAACACTGCAAAGTGAATTAAGACCAAAACTAAAAACATTTGGTGTAATTAACTTTGAGTACAAACGTGCAGTCAAAGAGTCCAATGTCTTTCGATTGGTGCATTTTGCTAAAAATATCTTTAAACCATTCTTGTTTTTTAACCTCACCAAGATTTTTATGAGAAATAAGGTCTATGAAGCACTTGATAGTGTTGGTTTAAAAAGAGAACATGCTTACAGATACCCCCATGAATTTTCAGGCGGACAAAGACAAAGAATTGCTATTGCCCGTGCTTTAATCACTAAACCCAAACTGATTATTGCAGATGAATTGATTAGTGCACTTGATGTTTCTATCCAAGCCCAAGTTATTAACATCTTGAAAGACTTGGCTAAAAAACACAACTTAACTGTGCTTTTCATTGCCCATGATTTATCAATGGTGCAAACTGTTTGTAACCGTTTGATCATTATGCATAGGGGCAAGATTGTTGAACGGGGCAGTGTGGATGAGATCTTTTCAAATCCAGTTCATCCCTACACCCGTTCCCTAATAAAAGCATCTCCTAAGTTAAGCAAAATCAATGTTGATCTCGCTTCTTTTGATGAAAACTTCACTTATGATAGTGATTATTCACTAACCAATATGCCCTTTTATATTAAAGTTCCTAACAGTGAAGAACATGAACTTTACTGTACTCAAAAGCAATTTGATAGTTGAATCAAAGAGGCTACGCCGATAAATTAATTTAAAATTTTCCAAAAATGTGGGAGCTAATTAAGCGTTTGTACCACAGCAAAATATGGCTAAAAAAACAGTTACAAGAATCGCTAAGATTAACCTAATTGGCGGACAAGCAAAACCTGGCCCTGCGCTTGCTTCTGTAGGGATTAATATGGGTGAGTTTACCAAACAATTTAATGAAAAAACCAAGGATAGACAAGGTGAAACGATCCCTTGTATAATCACTGCTTTTAACGATAAATCATTTACTTTTGTCTTAAAAACTACCCCTGTTAGTAACTTAATTAAACAAGCTGCTAAACTAGAAAAAGGTGCTAAAAATGCAAAAACTATTGTTGGAAAAATCTCCTTACAACAAGCTAAGGAGATTGCGCAATACAAGTTAGTTGATCTTAATGCTAACACAGTTGAAGCAGCATTAAAAATGGTGTTAGGTACAGCTAAACAGATGGGAATAGAGGTAACTGATTAATGAAAAAACTATCAAAAAGGATGCAAGCTGTTACCAAGCTCATTGATAAAAACAAACTTTATCCTATCCAAGAAGCATTTGAATTAATTAAAAAAACAGCAATTACTAAGTTTGTCAGTTCAGTTGATATTGCTGTTAGTTTAAACCTTGATACTACTAAAGCTGAACAACAGTTAAGAGGTGCAATTGCTTTTCCTTTTAGTATTGGTAAATCTATCAGAATTTTAGCTATCACTGATGATGAGAAAAAAGCTAGTGAAGCAGGTGCTGATTTTGTTGGTGGGCTTGATAAGATAGAAGCGATAAAAAATGGCTGATTAGATTTTGATCTAATTATCACTTCTCCCAAGTTCATGGGAGCATTAGGTAAACTAGGAAAACTATTAGGAACCAGGGGATTGATGCCAAACCCAAAAACTGAAACAGTTACTGATGATGTAGTTAGTGCTATTAAAGCTTATAAAAAGGGTAAGAAAGAATATCGAACTGATTCATTTGGCAACATCCACCTCTCTTTAGGTAAAACAGATACCAAAACTGAGCACTTGGTTGCTAATGCCATGGCTTTAATAGATTTAATTAAGTCTAAACGTCCTAGCACAGTCAAAGGTACTTACATTAAAAATATTGCTTTGACAACAACAATGGGACCAAGTTTAAAAGTAAAGCTACCTGATTAATGCCCACCTATAAACTAATTGTTGGTTTAGGTAACTTAGGTAAAAAGTATGAGAAAACTCGCCATAATGCTGGTTTTATGGTGTTAGATAGACTAGCTAGTTTATTCCACTTAAACTTTGATAAAACCAACAAGTTAGGTGATTATCTTTTTATTAAAGAAAAAGCAGCAATCTTAGCAAAACCTGCTACCTTTATGAATAATAGCGGTCTTTTTGTGAAATGGTTACAAGATCACTTTCAAATTCCGCTTGCAAACATAATGATAGTCCATGATGAAATAGCGTTTGATTTGGGAGTAATTAGGCTTAAAATGCAAGGGAGTGCTAACAATCATAATGGCATAAAATCAGTAATTAGACATTTAGATACTGAACAGTTCAATCGTTTACGCTTTGGGATTAAATCACAAAATACGAGTAACATATTGCATGAACAGGTAATGAGTGAATTCCAGAATAGTGAACTGACTAAACTGGAAGTTGCGATTACAAAGTCTGTTGAACTGTTGAAGCGTTATATTGAAGGAGAAGAGTTACAAAGGTTAATGGAATATTATCATCATGGCTAGTGAAAAACAATATATAGCAGGGGTTTCAGGTGGATCTGATTCAATGCTAATGCTTAAACTTTACCAAAAGAAGATTGCTTGTGTTGTTCATGTCAATTACAACACCAGATCAACCTCATTACGTGATCAAAAGTTAGTAGAACAATATTGTCAAAAATTAAATATTCCACTGGTTGTTCACACTGTTGATCCTGATCTAGTTTGAAAGAAGAACTTTCAAAATCAGGCACGGAAAATCCGCTTTGATCAGTTTAAAAAGACTGCAAAGCTATACCAGACCAACAAGTTATTATTAGCTCATCACCGTGATGATTTCATTGAGCAGGCCAAAATGCAACTAGATGCAAAAAAACGTGCTGTTTACTATGGGATTAAAACCAGGTGTGAATTGTATGGTTTGAAAATCTACCGTCCCCTAATGAAATACTGAAAAGATGAAATCATTGCCCTCTGTAGACAAGACCATATCCCTTATGAGATTGATGAAACTAACAAGTTACCTATTTATAAGCGCAATGAAGTGAGGTTAGAGATAGAAAAATGGTCTAAAATCGAAAAAGAACAGTTTTATATTGCTATCTGTGCAATGAACAAAACAATTGCTCAAAAACTGTTTGTATTAATGAAAAAAGCTAAAAAATGGTTATTACAACCTGATGTTAGGGAATTGAAACGGTTTTCAATTATTGATCAAAAACAGTTAATTTATAGCTATCTTATTTATCACAAGATTAATGTTAATGGTGAGAAAATTGATGCTATCCTTGATTTTATCCAACCTAGTCAACAAAAACAATACCGCTTGCAAAACGATATCTTTTTGATGGTGAAAAACCAGTGTTTAGCACTTTTATACAAAAGCTAAATGAAACATTTAACTGTCAAAGCATTAGTTTTGCAATTTAATGATTGTATCCAACTCATTGATGGTAAAAACAACATAGATAATGTGATTACTATCCCTGGGTTAAAAAGGAGTGTTTTTGAACTATTAGGACTATTTTGTAAGCCAATTGGTTCAGTTGCTATTTTAGGTAAACGTGAATTTATTTTTTTAAATCAAAAGCCAGTTGAGCAACAGAAAAAGATTATTGCAAACCTCTTGAAACTCAAACCACCTGCAGTTATTCTAACCAAGTCATTTCTTGATTGTGGTGTTTTGTTAGCTGTTAATCAAACTTATCAAGTTCCTATTTTAAAAACTAACTTGTTTTCAACTGAACTCTCTTTTACTGTTGAAACCTATATTAACGAGCAATTTGCTACTGTTCAAAAGTTACATGGGGTTTTACTTGAAATCTTTGGTGTTGGGGTATTTTTAGAAGGAAAGAGTGGGATTGGTAAATCTGAAAGTGCTTTAGATTTAATTAATAAAAACCACCTTCTAATAGGTGATGATGCTATTGAGATCTACAGATTAGGCAATAGGTTATTTGGTAGAGCACAAGCACTGGCAAAAGGCTTTATGGAGATTAGAGGTCTTGGCATCATTAATATTGAACGTGCTTATGGGTTACAGATTACAAAAGAACAAACTGAAATCCAACTAGCAATTAGTTTGTTGAGTTTAGAGGAGAAAAACAACGCTAGTTTTGAGCGCTTAGGCAGTGATTTAAAACTAAAAAATCTGCTTGGGGTTAAGATTAGTTACTATCAGATCCCTATCTCTTCAGGTAGAAAAACAAGCGAAATTATTGAAAGTGCAGTAATCGATTTTAAACTTAAGAAAAGTGGTTACAATTCAGCAAACGAGTTTATCTTAAAGCAACGTGCCATGTTAGAAGAACAAACTGATGAATAGACCAAGTTGATCAACTGCATTTAATATTGGTGGTGGATTTCCCATCCAGTGGTATGGGATCATTGTCTCAATTGGCATTATTTTTGCCATTTTAATGTTTGTCTTTAAACTGATTTATTGTTACAAATTACAAGACAACAGTTTTTATTTTTTTATCTTTATTGCTGTTTTAACGATGGTTTTAGGCGCTCGCCTCTGGTCATTTGTAATTGGTGATTCCAATTTTGCTAACAACAATTTCTTTGATTTTCGTAACGGTGGATTGGCCATTCAGGGTGGGATTTTGTTAACCAGTATTGTCGGAGTAATCTATTTCAACTTCTTTTTAAATAGTAAGACCAATAAAACCAAAACGATTGCTGAATTACTGAATAATAAGAATGAAATAAAAGCTGTTTATGTTGAAAGAAATATCTCTGTTCTAGTGATGTTAGATCTGATTGCTCCTTGTGTTTTAATTGGTCAAGCAATTGGCAGATGGGGTAATTTTTTCAACCAAGAAGTTTATGGGTTTGCTTTAGCTGGAACAATGAATGATCCCCAAGCATTGGCTAATACCCAGTGGGGATTTTTAAAGATCTTAATGCCTAAGGTTTGGGATGGGATGTGGATTGATGGTCAGTTTCGCATTCCGCTCTTTTTAATTGAGTCATTTTTTAACACTATTTTCTTTGTGTTAATTTACTTTGTAATGGATTTTATTAGGGGAGTTAAAAGTGGCACAATTGGTTTTAGTTATTTTCTTGCTACTGGAATCATTCGTTTAATCTTGGAAAACTTTAGAGACCAAACCTTTTATTTTCAAACTTCAATAACCACTAGTATTTTGTTTATTGTCGTTGGTATTTTAGGAATTTTTTATTGCCAGTTTATCCATGTCAAATTAAGAAATTACTTCTGAACTTATTTCTTTCTTTATGCCTTTTATAAAGTAGCTGCTTTTTTCACTACACTTTTTTTGAATAACAGAAAGCAAATGGCACAACAGAAGTTTGCTTTTTATGAAAAATCACTTCCCAATAAGAAGCGTTCTTTTTTTGAAATGAAGTATTACAATGATGTAACAACACCCAAAATTTATCGTTTAACTGATCAGGAAATGAAGTTATTTGATAAATTAGAGGCAGTTACAACCAGCTAGATTTTGTTAGAATACTTCAGTTGTCTATATGGCTACAATAGCGCAATTAATTAGAAAACCACGCCAAAAAAAGAAGGTTAAATCAAAGTCACCTGCACTCCATTATAACCTCAACCTTTTAAACAAAAAAACTACCAATGTTTACTCACCACTAAAGCGTGGTGTTTGCACCAGGGTTGGCACCATGACCCCCAGAAAACCTAATTCTGCACTAAGAAAGTATGCTAAGGTTAGACTTACAAATGGCTTTGAAGTACTTGCTTATATCCCAGGAGAAGGTCATAACCTACAAGAACACAGTGTTACTTTATTAAGGGGGGGTAGAGTAAAAGATCTCCCTGGAGTTAGATACCATATTGTTCGTGGTACTTTAGATACAGTTGGTGTTGACAAAAGAAGACAACAACGTTCTGCATATGGCGCTAAAAAACCAAAACCAAAATCTTAACTTGATCAGTTAAATAATGAGAAAAAATCGTGCTTTAAAAAGAACTGTTTTACCCGATCCTGTTTTTAACAACACACTGGTTACAAGGATTATTAATGTCATCATGAAAGATGGCAAGAAGGGTTTAGCACAACGCATCTTGTATGGTGCTTTTGAGATCATTGAAAAACGCACCAACCAACAACCTTTAACTGTCTTTGAAAAAGCAGTTGATAATGTTATGCCCCGCTTAGAGTTAAAAGTGAGAAGAATTGCTGGTTCTAACTACCAAGTACCAACTGAAGTTCCCCCTGACAGAAGGATTGCTTTAGCACTAAGATGGATTGTGATCTTTGCTAACAAAAGAAATGAAAAAACAATGCTTGAACGTGTTGCTAATGAAATTATTGATGCTTTTAATAACACGGGTGCTAGTGTTAAAAAGAAGGATGATACTCACAAGATGGCAGAAGCTAACAAAGCCTTTGCCCACATGCGTTGGTAGTTATTTATTATGTCAAGAACAGTTGATTTAAAAAACTTCCGTAACTTTGGCATTATGGCCCATATTGATGCTGGGAAAACCACCACATCAGAACGTATTTTGTTCCATTCAGGTAGAATTCACAAGATTGGTGAAACCCATGATGGTGAATCAGTGATGGACTGGATGGAACAAGAAAAAGAAAGGGGTATTACTATCACCTCTGCAGCCACTTCAGTGAGCTGAAAAAACTGCAGCTTAAACTTGATTGACACTCCTGGCCATGTTGACTTTACAGTTGAAGTGGAGCGTAGCTTAAGGGTTTTGGATGGAGCAATTGCGGTATTGGATGCTCAAATGGGAGTAGAACCACAAACTGAAACAGTATGAAGACAAGCTTCACGCTATGAAGTACCACGGGTAATCTTTGTTAATAAGATGGATAAAACCGGTGCTAACTTTGAGCGCTCTGTTTTATCAATTCAACAACGCTTGGGAGTGAAAGCTGTTCCTATTCAATTTCCCATAGGTGCTGAAAATGATTTCAATGGCATCATTGATATCATCACTAAAAAAGCTTATTTTTTTGATGGTAATAAAGAGGAAAATGCTATTGAAAAACCAATTCCTGAACAGTATGTTGATCAAGTTGAAAAACTTTACAACAACTTAGTTGAAGAAGTTGCTAGTTTAGATGATCAACTCATGGCTGATTATCTAGATGGTAAACCAATTGAAATTGATGCAATTAAAAATGCAATTAGAAACGGGGTAATTCACTGTAAGTTTTTCCCGGTATTGTGTGGTTCAGCATTTAAAAACAAGGGAATTAAACTCTTACTTGATGCAGTGGTTGATTTTCTCCCTTCACCTGTTGATGTCCCACCTGCTAAAGCAATTGATGCAAACAACAAAGAGATATCTATTAAAGCTAGTGATGATGCTAACTTTATTGGCTTAGCATTTAAAGTTGCTACTGATCCTTTTGTTGGTAGATTAACTTTTATTAGGGTTTATGCAGGAGTTTTAAAATCTGGTTCTTATGTTAAGAATGTTAGAAAAAACAAAAAGGAAAGGGTATCACGTTTAGTGAAAATGCACGCACAAAATCGCAATGAAATTGATGAAATTAGAGCAGGGGATATCTGTGCAGTAATTGGCTTGAAAGATACTACTACTGGAGAAACTTTAACTGATGATAAGCTTGATGTGCAACTAGAAGCAATGCAATTTGCTGAACCAGTGATCTCTTTAGCAGTAGAACCTAAAACTAAAGCAGATCAGGAAAAGATGTCAATTGCTTTATCAAAACTAGCAGAAGAAGATCCTACTTTTAAAACCTTTAGTGATCCTGAAACAGGGCAAACTATTATTGCTGGAATGGGTGAGTTACACCTTGATATCTTAGTTGATAGGATGAAACGTGAATTTAAGGTAGAAGTTAACATTGGTGCACCTCAAGTTAGCTTTCGTGAAACCTTTAAATCAACTAGTGAAGTTGAGGGTAAATACATCAAACAATCAGGTGGTAGAGGTCAATATGGACATGTTAAAATCCGTTTTGAACCTAATAAAGATAAGGGCTTTGAATTTGTTGATAAGATTGTGGGCGGAAGGATTCCAAGGGAATATATTAAACCAGTTCAAACTGGTCTTGAAAATGCAATGAATTCAGGTCCTTTAGCAGGTTACCCAATGATTGATATTAAAGCTACCTTATTTGATGGTTCTTTCCATGAAGTTGACTCAAGTGAAATGGCTTTTAAAATTGCTGCATCCTTAGCTTTAAAAGAAGCAGGTAAACAATGTAACCCAGTTTTACTTGAACCTATTATGGCAATAGAAGTTACTGTACCTGAACAGTACTTTGGGGATACAATGGGTGATATCAGTTCAAGAAGAGGGATCATTGAAGGTACTGAACAACGTGATAATGTTCAACTAATAAAAGCAAAAGTACCTTTAAAAGAGATGTTTGGTTATGCCACTGATTTACGCTCTTTTTCCCAAGGTAGGGGTAATTATGTAATGCAATTTAGCCATTATGCTGAAACTCCTAAAAGCGTTGTTAATGAGATAATTGCTAATAAAAAATAGATACTTAATAAAAATATAACTTTACTCAATGAGTTTTTCCAAAAAGTTTTTATGCACTACAATATCATTCTTTTAGTTGATGGTACGCTTAGTTTAGAACAAGCTAACCAAGTTGAACAAAAACACCAAAAATTGCTTGAAAAGGCAACTGAATTTAAAAGTGAATACTTAGGTTTAAAAGAGTTGGCTTACCCCATTAAAAAGCAACTTTCTGCTCACTATTACAGATGGAGTTTTCATGGTGAAAGCAATTGTACTAAGGAGTTTAAAAGAGCTGCTAACATCAATAAGCAGATAATAAGAGAGTTAATTATTAACAGAGAAAAAGACTATGGTTATTTAGGTTCAGTTAACCCTAAAAAACAACAACTGTCTTTGCAGAAGCTAACCAAGTATAATGAGATTATTGCTAGTGAAAATAATCCTGATAACCCAGATGCGCCTGTCACTTCTGGTCTAGCTTCTGTTAAACCACGGCTATCAAGAGTTGAAAAACAAAAGGAACGTGAACTTGAAAAGTGAACGGTTGTTCACCAATCAGGTAACTTTGATACTGTACAGATCAATCCTTATCGTCCTAGGATAAAACGCTTTTTACAAAACAACCAACAAACCTCCCAAGCTAATAATAACCAACCTCGTTTTCAAAATCAATTTAAAAAAGGAGCAAAACCTTAGATGAACCGGGTCTTCTTGTTTGGTAAACTCAGTTTTACTCCCAACCGTTTACAGACAAAAAATGGTACGTTAGGAGCTACTTTTTCCATGGAATGTCTTGATTCCAGTGGTTTTAATAATGCCAAATCATTCATTAGAGTAACTGCTTGAGGTAAAGTTGCTAGTTTTATTGTTGCTCAAAATCCTGGGGTGATGCTTTTTGTAGAAGGAAGATTAACTACATATAAAATTACTAACAGTGAAAATAAAAACACCTATGCTTTACAAGTAACTGCTGATAAGATCTTTCATCCTGATGAAAAAACTACCAATGAAGAACCTATTAAATCAACTGTAGTTGATTCACCCTTTATGAATCCCAAAGCAAGTGTTACAGAAGCTGAGTTTGAACAAGCATTCCCCCATCAAGATGAAACTGATTTTAACAACATTACCCCTATATTTGAAAATGATGTCCAACTAGAGGAGGAAAGTGATGATTAATAAAGAACAGGATTTAAACCAATTAGAAACCAACCAAGAACAGAGTGTTGAACAAAACCAAACTGATGAAAAGCGCAAGCCAAAACCAAACTTTAAAAGAGCAAAAAAATATTGTCGATTTTGCGCCATAGGTCAACTAAGGATTGATTTTATTGATGATTTGGAAGCAATCAAACGCTTTCTCAGTCCCTATGCAAAGATTAATCCTAGAAGAATTACAGGTAATTGCAACATGCACCAACGTCATGTAGCTAATGCTCTAAAACGAGCACGTTACCTAGCTTTAGTGCCATTTATTAAAGATTAAATATGAAGATAATTTTGAAGCAAGATGTTGCTAAATTAGGCAAGCGGTTTGATGTTGTTGAAGTTAAAGATGGGTTTGCTATCCATTTTTTATTTCCCAAAAAACTAGCTGCACCTTTAACAAAGAAAGCAATTGCTAACCGTGATTTGTTTTTAAAACAACAACAAGAACAATACCAAAAAAATCGTGCCTTAGCTGAAAAATTGAAACTAGTAATTGAACAAACACCATTAACTTTTCAACTCAAACAACACGATGGCAAGCCATATGGTTCAATCATCACCAAACAAATAATTAATTTAGCAAAACAACAAAGACTTGATTTACAGCGCTTTATGTTTAAAGATAATGTGCGCTTACAGTTTGGTGAACACAAACTAATTTTGCACCTTTTTGAGGAGATAACTGCAACTTTAACTGTTATAGTGAACCCTGAAAATGGGACAACAAACTAGTTTTAAATATGCAAATGATAGCAACATTGAACGTGCTGAAAGACGTTTGATGCAAGCAGTTGCTCAAAACAGTGAGGGCATTGATCTAATTTTCAATAAACTTGAACCAATTGATTTTTTTGCAACCCCTTTCAAACTCATTTTTCAAACTGCAAAAGAAAACTACCAATTAAATAACCCTATTATTGGTTCTGGTTTACTAGAAGCGGTTAAGTTTAAACTTGATGCTAATGATCAATCCACTAAAAGTGAACTTGAAATTTTATTCACAAAGATCTTATTAATCCGTTTACCACCTAACCAAACAGAGATTAAAACACTGGTTGATGTTGTTAAAAAAGCTTCTATTTTTCGCAGGTTACAACAGTTTGCTAAGCGTGTTTACAACGAGGAATTTAAGTTAAAAGAAGATCGTTTTGAAGGCTATTTACAAGCTATTCAAGATGATTTTGTCAAGATTATCCACAGTGCTTTTAGTAACATCTTTGCTTTTAGCTATGATGAGATTGCCAATCAAGAGGAAGCATTAATTAAAAAGGTTCACCGTGGGGAATTGATCATCAGTGGACTTTCAAGTGGATTTTTAAAATTAGATCAACTTACATCAGGTTGAAAACCAGGAGAGTTAATAGTAATAGCAGCTCGCCCAGGTAGAGGTAAAACTGCCCTTTTGATTAATTTTATGGCTAGTGCAGCTAAACAAATTGATCCTAAAACTGATGTGGTCCTCTTCTTTAGTTTAGAGATGCGTAACCGGGAAATTTACCAAAGGCACTTAATGCATGAAAGTCAAACTAGTTACACACTAACCAACCGGCAAAGGATTAATAATGTCTTTGAAGAGTTAATGGAAGCATCTTCAAGGATCAAAAACTTACCTATTAAACTCTTTGATTACAGTAGTTTAACACTCCAAGAGATCAGAAACCAAATTACTGAAGTGAGTAAAACCAGTAATGTTAGGTTAGTAATTATTGACTATTTACAACTTGTTAATGCTTTAAAAAATAACTATGGTTTGACAAGACAACAAGAAGTGACAATGATCTCTCAATCACTTAAAGCATTCGCTAAGGAGTTTAATACCCCTATTATTGCTGCAGCTCAACTTTCTAGAAGGATTGAAGAAAGGAAAGATTCCAGACCAATTCTTTCTGATTTAAGAGAATCAGGTTCAATTGAACAGGATGCGGATATGGTTTTATTTATCCATAGAACTAATGATGATAAAAAAGAACAGGAAGAGGAGAACACAAACTTGTTTGAAGTGGAGCTTATCTTAGAAAAGAACAGAAATGGTCCCAATGGCAAAGTTAAACTAAACTTTCGCAGTGACACTTCTTCTTTTATTAGTCAATATTCCCCTAGTTTTGATGACCAATACAGTTAATTTATGAAACTGAAGTTTTACAAACTACCTTTAATTACTACAGCATTTAGTTTTGTATTTTTAACTGCTTGTTCAACACCCCAATCTTCCTTTTTTCTCCCTGCACAAACAACTATTAGTACTTTAAAAATTAATGGTATGGAAAACAAAACAGGTTATTTTCTTGAAACGCAGCGTTCTCGTGGTAGTTACAATCCCACCGCTTCACTTACTATGATTAAGTTAGGAGATGAAAAAGAGTTTAAAAACGTAGATACAACCAAGCAAGATGAAGTTTTGTTTGCCAACATTTATGGTGGAATCTCTTCATTGCTTAACTTCAGAATTATCCAACCGATGTTAACCTACTGAAACTTAGTTAACAACTCATTAAGTCAGATTGGTAGTACTAATGACTTAATTACATTCAAAGATAGTGGATATAAAGATCAACTTGCAAAAGCGCTTGCTAACAATCTCATAGTTGCTGATGAAGGTAATAATAACTTTTGGTTTGGTTTAAAAGCCCTAAAGTTTGATACGGTTAAACTCCAAGCTAATAATACTGCTACTAGTTCAACAAGAGCTTCAACTACTCAAAATACTAATAATAAGATTGATGCACGTGAAAAAATCACCATTAATGGGAATGGTGGAACAAATAATGATCAAAATGCTACTGTTCAAAAGTTGATAGGTATTGAAAATATTGAAGTTGAGTTTAGCTTTGTTAAAACTGGTTTTAATGGAAATGAGATTAAGTTTGAAGATTATGTAACAGATTCTTCCCCCACAACATCTTTATTAAAGCAAGTTTGAAAAAGTAAGTGAAATACCGAGTTGACCCACACCAGCTTTAAATTCAAACTCAATTCTTTCAATGTTTTGTTAACTTATCAATTGGAAGCAAACCAAAAAAGCCAATATCTTCCCAATGGCTTTAGCTTTCTTTTTCCATCTAATTTAGAAGGAAAGATAGATAGTTCAAAAAGCTATTGAAACAATCTTGTTGATTTTTCAAAAAGAACCACAAATGAAGAGAACACAATGCTACTAACTGATTTGCAAAAGAAACAAGATCAAGTGAATCGGTTTGTTGGTTTTATTGGTCAAAACCACTTTACTTTAAGTGCTAACAGCATTAATGAAAAGCAGTTTAACGATGCTAGTACTGCTGATTTTTTTAAAGCAATCTTTCAAAAAGTGAGTATCAATGAATAACAATTTTAGCCATTCAGTTTTGAAAAGAAAAGTTTAAATTAATGGTAAAATTATTCATTAAACTTTCCTTGTTAGTTGTAGTTTTCAATACTGTTTGTATTTCGAAGTTCATTTTAAAAAATTCGTAGTTCTAGATCTTTAAAATATGTTTATTTAATTGTCTTTTTTAATTTCATAAATTGTTTGAAAACACAAAAAAATATCTTTAAGGTCAAGGCCTTACTACTTTCACTTTTTTCTGCTGCTGTTACCATCACTATTTTTGCTTTACCTATCTTTGCAAACAATGGTTCTAAAACTGATTTAGGTTTGCTTTCTAAAAATAGTGCTGATTTTTTAGGTAGTTCAAAAAGAAGTCTTGCTGGCTTTGATACTCCTTTTAGTCCAGATAACCTCCAGTATTTAGAAAAAGAAACTGATTATGATCAGAACTTTAAAAGTTTTACTGAAAAGTTTAAAGATGAAAAAATAACTAACAACCAACTTGGTATTGTTGATATCTATAACTTATTCAGTGGTTTTCACAAGAGTGTCAAAAGCACAGTTGACTTAATGAACCAACTGCAAAAGCAAGTTGAAGCTGCTAATGCTATCTTCCCAGTTGATGATGCTTTTGTTAAATTACCTAAAGTACCTACTGAATTATTTAAGTTAGTTGATGATAATGTCTTTCCTAAGTTAAATCCTAAGGGCTTAAATATCTCTGATAATATTGCTGCACTTTTTGAAAGATATAACCTTAAATCGATTGAACTTAAGAACTTTGACTTAGCTTTCTTGAGAAAAGCTGATGTAATTATCAAAGACAAGGTTCGATATAACTTTGAGATGCAAATGCAGTTTCAAACTGTTTATGTTGGTGGAGGGAATACCGTTATTAACTTAGACTTTACTTTAAAAGCCCAAACCGTTAACTTTGCTAACCTCCAAGATTTACAAAACACTTTTGTTAAAGTTGGTAATGATCTTTCCACCCAACTCTTTTGGATTCCAACTGTTAATAAATTAACTGATAATGCAGGTAATGATCTTACCCATATTGCTAAAACTGTGATTGGTGAATCGTTTTTCCAAACCAATGTTAACTTAGCTAAATCAGTTATTGAATATGATAAGGTTCAACCATTGGTTAAACAAGCTTTTGAAGAGCGAGTTTTAACTCCTTTCAAAAAGGAAAGAGAAGCTGCTAAAAAAGCTTATGAAGAAGAACAACGTCGCTTGGAAGAGGAACGTAAGCGTCAACTAGAAGAGCTAAGGAGAAGAGAAGCTGAGGAGAAAAGAAAAGCTGAAGAAGCAAAACGAAATCAAGAAAAAGCACGCAGAGAAAGAGAAGCTTATGAAAAATCATTTAACTCCTTTAAAGACTTTAAATTTTACTGGTTAACTAAAGGTAAAGATGTTACTAAAAAAGCTGATTTAATTGATGCACTTAAAACCGCTATTGCTACACCAGCATACAGAAATAGAACATTCTCTTTATTAATCAAAGGTTTTGCTAGTGGAGTTGAACGTTATTTCAACGCTAACAAGAATGATAAAGAGTTGAAAAAACTTGCATTTGGTGAAAAAGGGATCCAATTCCCTAGAGCAGATGCTGGGGTAAATGGTCTTTACATGAGTAATTTTTTGAGGCATGAATTGACAAGCAAAGCAAAATTTTCACTTAACCTAAAAGATATAAAAGTTGAAAATACGGTTGAAGATACTCAATTGTATTGGAAGGATAATGGCATTCATTTAAAGCAAGCCAATCCTTATAAATTCAATCTAAATATCAAGATCAAGTACAACGGCTGGTATAATGTCCACTGGTGAAACTGGCTCCCTGCTAAAATTTTAGGGATTCCTACAGACTGAAGTGGTGAGATGAATTTAACATTTGTTGTCAACGGTGACCTATCAGAAATTGTTGATAAACATGATTACCCTGGTACTTTCTTTCAATTTACCGATAAAAATGAATTGCTATTTACCTTAGCAGTTAGAGAACAAATTAAGGTTGATAATAATCATTTTATGGGTCTGTTAAAAAGCCAAAACCTTCATAATCTTCAGCTTGCTTCTGGTGCTACAAAACCTCCTGTTGTTGATTTAGCTAGTTATTTCCACTTTGTATTATTGACTGAAAAATCTTAATCTATTAAGAAATTTTTGATCAGTTACTTTTACCTAGATAAATTTAAAGTTTATTTGTGGTAATGGATGATCTATTCCAAAGAATGGTTAGCTGTGTTCTACCGTCATGAAGAGCTTTTATTGATGAGGAAGTTAAAAAACCTTATTTTCAAGCTTTATTAGAAAAATTAAAGGCTTTAAAAGCAACAATAATTCCAAAACCAGAACTTATTTTCCGTGTTTTTAGCTTCTTTAAGCCAATTGATACAAAGGTAATTATCTTTGGTCAAGATCCCTATCCTAGTCCTAATGATGCTTGTGGACTTGCTTTTGCATCCAATAATTCCAAAACCCCTGCCAGCTTAAAAAGAATAATTTTACGTTTAGAAAAAGAATATCCTTCGCTTAAACAAGAAAGTAGTTGACAACAAAACTTCCTATTGAATTGAGCAGAACAGGGCGTTTTATTACTAAATGGAATTTTAACAACTACTGTATTTATACGCAACGCCCATAAAAATTGGGGTTGGGAGGAGTTTAACTGTAATTTGCTAACTTTTCTAAAAAATCAAAACATTAAACCGCTGTTGGTATTTCTGGGTGTTCAAACTAAAAACTTTGTTGTTAAGAGTATTGGTAATGTTGATGGATTTGAGCATTTATCATATCCCCATCCCTCACCACTAAGTGGTAATTTGTTTCTAACAAACCCTAACGATCTGTTTAAAACAATTAACAATTGGTTGAAACAACATAACCAAAAAATAATTAACTGAGCAGTTGTTAAAAATGCTAGTTTTGACCAATTAAGTTAATTAAAAACAAAGCTGTAGTTGACACTTGTTTATTGATTAGAAAAACAACAACCTTGTAAAATTATTTAACTAAGTGATTATTACTTAATTGCACTTTAGATGGGAGAGATTAAAAATACTGCACCAACAAGCGATATTTCCACTTCAGGATTTATCTATTTTGCAGTTGTTTTTCTAATCATTATAGTCTATCTTTTTTTTAAAAACATTCTCTTTTTGTTCTTCTTTAAAAGATATCCTAAAAACACACCTAAAATTGGGGTTAGCAATATTACTACTATTGCTATGATTATTGCTGTTGCTGTATCTGTTGTTTTGGTCTTAATGGCTTTAGCAGGGGGGTTAACAGCAGCGCTGTTCCGTGGTTATCCTGGGTTCCGTGTTACCTTAGAGTTAATTCTAGTGAAGATTTCAGGACTTTTATTTGGTCCTATAATTGGTATTTTTTCAGCAGCTACCATTGACTTTTTAACTGTTATTTTTTCAGGTGGGGTGTTTAATATTGGTTATGTTTTAGGAGCAATCCTAACGGGAATGATTGCTGGAATTTTACGTGAAGTTTTAATTTCAACTTCTTTTTTAAATAACAAAACTTTAAGTGATTTTGCCTATCTTGTTTTATCAGTGGGGATGGTATTTGCCAGTTTTTTAGTAACCCAGTTTTTTGTTATCTCAGTTACCCAAAACTTATCAGCATTTCAAAGTAATGATCAAATTGTTTTACGTTTTAACGCCTCACCTTTAAATTTCAGTATCTCATTGCAAAGATATGTTCAAATTATTTTCTATTTTGCGATGGTTGTAATCATCACAATGGTAGTACTTTACTTTGTTTGAATAATCAAGCAAAAGCACTTTAACTATGCTTATTCCAAGTTTTTCTTTCGTAGATATAAACATGCTAATCACCAATTTACCTTATTTGTGTTAACAAAAGAAAACTGGTTTTATCTAATTCTGAATGTAATTACATTAGCAACCACCAGTTTGCTAATGATTAACATTGCATTTATTCCTATCTTTGATACCCAAACAACTGGACAAACTTATGATTTTTGATTATTAGTTAGATTATTGTTTGCTCCTTTGATCTTCTTACTTGATATTATTGTTATCTATCCAATCTTGTTATTGTTAACCCCAATCATGTTAAAAGGGTTTAAAACAGTAGCATCAGAAACCCAAACAAAAGGAATCAAAAAGAGTTTTTCAGATATGCAAAGCTTAATTATGCCCAATGTTATTAGTCACAAAAAACAGCAGTTAATTAGAAAAGAGATGCAACAGTTAGCAAAAACAATCAGAATTGATTTATCAGACAAAGAAGTGGATGCACTTGTTGAAGAGTTTAAAGAGATCACAAAGAGTTTTAATAAGGTAACTAAAATTGATACCACTAATGTTCAACCGATGTACGCTCCATTTGAATTTAGTCCAACCCCACTAAGAAAAGATAAACCAGTAGTTGATAAACACGCTAAGCAACTACTTAATAACTGTTGTGAAGTTAAAACAGGTTTTGTAAAGGTATAGTTGTGCGATCAAATATTTTAAGTCTCAGGGCGATACTTGATAAAAAACCTAGTGCCATTAACGATGTTTTAACATCAATTAATGCAAAGATAGAACTGAATAAATCAAGTAATTTTTTATTGAAAAATACTGTTGAAATTTATTCAAAAAAAATTAATAAAAGTGATGAAAAGATTCTGCTAAATAACATCCCTTATGTTTTGAAAGATAACATCGCTACTAAAGATATTGTCACCACTGGTGGTTCTTTGTTTTTAAAAAACTATCTTCCCCCTTTTTCAGCAACTGTGTTTGAACTGTTAGAAATGAATGGCGCGTTGCTTGTTGGTAAAGCTAATATGGATGAATTTGGCTTAGGTGGAACAGGTAGTTATTCTGCTTTTGGTGTTGTTCATCACCCTGAAAATTCCAGTTTAATTGCAGGTGGTTCTTCCTCAGGTTCAGCTTACGCAGTTGCTAAAGACATTGTTCCTTTTTCCATTGCAACTGACACTGGTGATTCGATTAGAAGACCTGCTAGTATCTGTAATGTTGTTGGCTTTAAACCAACTTATGGTTTGATATCACGTAATGGGGTATTTCCATATGCCCCAAGTATGGACCATGTGGGGATATTTGCTAAGTTTGTTAGTGATATTGCCATTGTTAGTGATGTTGTTATTAAACATGATAAAACTGATTTTTCTTCCCAAAAATCACCTGATGAAAACCAGTTTTTCAATGAGTTGGCCATTCCCTTTACAAGATCAATTCGCTTCGGTTATTTAAAACCACTAGAAAAACTGTTTAACAAACACCTCCAAAAAAAATGAAATAATCTCAAAAAAACCTTAGAACAAAAAAACTACCAGTTGATTCCACTTGATTTTGATGTGGAACTTCTCAAAGTAATTGATTCTATTTACAAAATAATTAGTTATAGCGAAGCAGTTAGTTGTTATAGTAATTTAACTGGCATTGTCTTTGGTCAAAAGGTGTTTGAACCTAATTCACCAAGTAATTTTGATCAAACTATTACCAGAAACAGAGATCAGTTTTTAGGTAAACAACTAAAAAGAAGATTTGTAATAGGGGCATTTGCAACTGATGAGAAGAATTTTGAAAAGTACTTTGAAAAAGCTCAGAAAATAAGAAGAGTCTTAGTGGATAACTTTCTGAATCTCTTTAGTGATGTTGATTTTGTATTATCACCAAGCGCTTCTTGTTTTGCTAGTACCATTGAAGATATTCAAGCTAATAAGCCATATACAAACATTATTGATGACTTTTTACAATTAGCTAATTTTGCTGGTAGCCCTTCTATAACTATCCCTTGGTTAGTTCAAACAAAAGACCAAACAATTGGTTTAAGTATTAGTGCTAACTGTTTTGAAGATAAAAAACTCTTACAAATTGCTTATTGATTTGAACAACTTTTTGATTTAAACCATGATTAATTTTGAAGCGATTATTGGAATTGAAGTCCATGTAGTTTTAAATACTGCTAGCAAGATGTTTTCAAAAGCACCTAACCGCGTTGATAATCAAAAAATCAATCATTTTATTGACCCAATAGATTTAGGTTTACCAGGCACTTTGCCTCAAGTTAATGAGTTAGCAGTTTACAAGGCATTATTATTAGCTGATGCATTAAAAATGAAGACAGTAACAAATAAACTTGTTTTTGATCGAAAGCACTATTTTTATCAGGACTTACCTAAGGGTTTTCAAATCACCCAACAAAATTATCCTTTTGCTAAAAATGGTGTAGTTACCATTAATGTTGATGCTATTGAAAAACCAATTTATATTGACCGGTTTCATTTGGAGGAAGATACTGCCAAACAACACTTTAACCATGACCAAATTCTGCTTGATTTTAATAGGTGTGGTGCACCTTTAATTGAAGTTGTTACTCTTCCTGTTATCAACACTGCAAAAGAAGCGAAAGCCTACCTACAGAAGTTGAGACAAATTCTGATTGTTAACAATATCTCCAATGCCAAATTGGAAGATGGTTCAATGCGGAGCGATTGCAATGTTTCAGTACGTTTAAAAGGTCAAAGGCAACTAGGAACTAAAATTGAAATTAAAAACATCAACTCACTTAATAATGTTGAAAAAGCGATTGATCTTGAGATTAACCGCCAAGTTAAAGCACTTATTAATGGTGAAACCTTGAGTCAAGCAACTTTAAGCTTTGATGATAAAACCAACAACAATGTTTTTATGAGAAAAAAAGACAATACGATTGACTATAGGTACTTTATTGAACCTAACATCATGACTAGTAATATTGATGATTTATTATTAGAAAAACCTGTTGCTTTTCAGTTAGAACAGTTTCAAAAAGAACTAATTGATAGTAATGTCAATCCTCAATTAGTCCAATTAGTAGTTGATGATGCAACTATCTTTAGTGCTTTTCAAACTATTAATAGTGTGATTAAAAACCCCCAAGAAACCATCAGGTGGTTATGTATTGAACTAATAGGTCAACTCAATAAAACCAACAGTTCATTAACAGCTAATTTAATTCAAGATCTAATTACCCTAATTGAAATGCTAAAAGCAGCAAAGGTTAACCAAAAACAAGCTAAGCAGTTAATTACTTTAATGATTGAAACTAAAAAAGATCCAAAATCGCTAGCTAAGCTCCATAATTTAGAGCAAATTACTGATCCAAAAGAGTTACAAAAGATCATTAAAAAAATCTTTCAGGAAAATGAAAAAGAGATCCTGAAAAACATTGATAGAATTGAACGGATTCAAAAGTTAATTATTGGTCAAGTTATGCATAAGACCAATAATCGAGCAAACCCCCAACAAGTTTTTATAATTGTTGAAAATATGCTTCATGAAGTTCGGGAAAGAGATAGCTAAAAAAAATCAAATTATTTATCGCTATATAATCCTTAAAATTCAAAGCTTTGAATGACCTGCTAACACCCGTATCTTCTCAGAACGCCAACTAGAAATCCGTTTCAACTCCTCACGGAGTCAAATTCGCTCTGTTCTCGCTACGCTTTTAAACAAAAATATAATCCGCTACACTAAAAATACCCCTGGTTATTTTGTGTGTAAAGATGTAGGCTTTAGTTTTTTTCATAAAACCCAGGATAATCTGAAGGTGAAATATGCTAAACTTTCAACTTTGATTAAAAAGCTGTTATCACAGGATGATGCTAGTGTTTTTGCCAATATTGACAGTACTGTGCACCTTGATAAATTTAAGGGAATTGAAGCTAAGTTTTTTGATGAGAATAAAAAACACTTTTTAAACGTTTGTTTTTTTGCTAAAGATGATATTTTAAACATCCTTGATGAAAACAACCTCCAGCAACAGTTCTTCAGGGAGTTTGCTTACAATGGGATTGCTATTGAAAAACGTCATTGTGTTACCTCTGTTGACAAGGAAAGTGGGTGCTTGGTGATGTATGACATGTATTATGATGACAATGATAATTTTGTAGTTGCTAGCAAAAGTAACTTCCTTAACCCTGAATTGAAAGTAATCAATGCTTAAAGTGAATGCTGATTTTTTAACTAAAGATCAAGTTATCTATGATTTAGTGATAGTAGGTGCTGGCCCTGCTGGGATTGCTAGTGCCATTTATGGTAAACGTGCTAACTTAAATTTAGCAATTATTGAAGGAAACACTCCAGGAGGGAAGATAGTAAAAACTAACATTGTGGAAAACTATCCTGGTTTTAAAACCATAACTGGTCCTGAATTAGGTCTTGAGATGTACAACCACTTGTTAGCATTTGAACCAGTTGTTTTTTATAACAACTTAATCAAAATTGATCATCTTAACGATACATTCATCTTGTATTTAGATAACAAAACGACAGTTTTTAGCAAAACTGTTATCTATGCAACAGGGATGGAAGAGAGAAAACTTGGCATTGAAAAGGAAGATTATTTTTATGGTAAAGGGATTAGTTATTGTGCTATTTGTGATGCGGCTCTTTACAAAGGTAAAACAGTTGGTGTTGTAGGAGGAGGTAATTCTGCAATACAGGAAGCAATTTATCTTTCAAGTATTGCTAAAACAGTTCACCTTATTCACAGACGTGAAGTGTTTAGAAGTGATGCATTACTAGTTGAAAAATTAAAAAAAATTAGTAATGTAGTTTTTCATTTAAATGCTACTGTAAAACAGTTAATAGGTCAAGAAAAGCTCCAAACTGTTAAATTGGCAAGCACAGTTGATAAATCAGAAAGTGAAATTGCAATTGATTGTCTCTTTCCTTACATAGGCTTTGAAAGTAATAACAAGCCAGTTTTAGATCTTAAGCTTAATTTAGATCAAAATGGTTTTATTTTAGGAGATGAAAATATGCAAACTAACATTAAGGGTTTTTATGTTGCTGGGGATTGTAGAAGTAAATCATTCCGGCAAATTGCCACTGCAATTAGTGATGGGGTAACAGCTGTTTTAAAGGTTAGGGATGACATTTAGTACACAGATTAAAGCTGAACTGGTCCAAAACAAATTAATTGATAAACACTGAAATGTCTTTTTAGCAGGTTTTTTTCAAAACAACTTAAAGCTACTTTACAACCGTAACTGAAGTTTTAAAGTGCAATCTGAAGCATTAAAGGAACAATTTGTTCAAAATCTTAAGTTTGACTTTAAGACAAAAGCTAGTAAGAAATACTTTCTTTTTGAGTTTAATGCAGATATTAACGTAATTAACACTCTTTTAAAACTTGATGTGACAACTAGTGAATTGGTAGTTAAACAAGTTTATCTCATTGCTGCTTTTTTAAGTGGAGGTAGTGTTAGTGATTTAATAAACTCCAATAACTTTCACTTGCAAATCAGTTCCAACAATGAGTTTCAAATTCAACAACTTTTAAAATTGTTTAGTTTTTTTAAAAAAACAGTTAAACAAAACCAGTTAGTTGTTTATCTTAAAAGTTATGAAAAGATCTGTAATTTTTTAAAACTGATTCAAGCCTTTGATGGTTATCTTGCTTTTGAAAATAAGCAACTAGAGAAAAGTTTTACTTTAAACCAGTTAAGAAAAAGTAATTTGGAAGTTGCTAACTTAATGAAGACAATCAGATCTAATAATCAAACTAATCAACTCCAACTAAAATCATTTATTAAAAGTAGTAGTTTTGCAAAAAGACCGCTTAATTTTCAGCGTTATTGCTTAATTAAAAGTGATCATCCTGATTGATCTTTAGAACAGATAGCAAACTTTTTTTTCACAAAATATAACATAAAGATTAGCCGCAGTGGAATCCAACATTTTAGTGTTAATCTAAAAAAACTATGCCAGTAGTTTAAAATTAGTTCACAAGCAATGCATCCAATCCAAATAGTAATGTTCATTATGGCTGTTATCTGTTTAATTATTGGACTTTTGCTTTCTAACCATGGTTCTACTGGAGGATTAGCTTCTCTATCAGGTCAGGACTTGGAGATCTTTCGTAAAACCAAAGATAGGGGTTTTGTAAAGATCTTACAGATTATCATGTTTATCTTAGTAGTTTTATTTTTAATTCTTGGGTTGATATTTAGTTTTGCACCAAGATAACAATGAAGGTTTTAACTGAACTCCAAAAGCAGATATTTACCATTGTCAAAAAGGAAAATGGTAAACCTATTCCCCCTGGAATAGTGGTAAGAATGATGGAAAATAGTCCTAATTTTCCAGGTAAACATCTCATCTATCGGGCCATTGATGATCTGCTTGATTGAGCCATCTTAAGGAAAGCTGGTGGGGTTACAAACCAGCTATTAGTTAACTATGAACCTGCTGAGCCTTTACTTGATAAAAAACTACAAGGGATTTTAACCTTAGGAAATAAGAATAGTGGTTTTATCCGCTCTTTGGATGATGATAAAACTGTGTATTATGTCCATTACTCTAATTTAACTGGAGCTTTAGATGGGGATCTTGTGGAGTTTTGTAAATTAGATAAACCCCAATTTGGTGATAAGTTTGATGCTGCAGTTATTACTATTCTAAAAAGAGCAAGAATCTTGTATGCAGGTAATTTTTTAGTAGATCAAAATGAGTTTGCCTTGGAATACAAAATTGTTGCTGATAACCCTAGATTTTATTTAACTATGATTGTAAATCCTGATTCTATCCCAAATAACTTAGCATCTAACACCAAGATAGCTTTTCAAATTGATGAGTATGATCCTGATAACAACTTATGTAAGGTTTCTGTACAACAAGTTTTGGGTAACAATGATGATCCGCTAATTAATATAAAAGCAATCATGTTGGACAATTCCATTGTCTTTGAAACTAACGATGTAGTTGAACAGCATGCTAACAAGTTAAGTTTTGATACTGAAGAACAACATAAAGCTTACCGTCAGGATTTAACTGATTTAGCTTTTGTGACTGTTGATCCTACAACATCAAAAGACCTTGATGATGCTATTTATGTCAAAACAATACCAACAGGTTTTGTGCTTTATGTAGCTATTGCTGATGTTGCACACTATGTTAATAGAAATAGTGAAATAGACATTGAAGCAAAACACAAAACAAGCTCAATCTATCTACCTGGTCATTATGTTGTGCCCATGCTACCTGAGCAATTGTCAAATCAGCTCTGTTCTTTAAATCCAGCACAAAAACGTTATGTTGTTGTTTGTGAGATTAGTTTTGATAATCAGGGAAGGATTAAAACAAACAAGCTTTACCCAGCAACAATTATTTCCAAAAATCGTTTTAGCTATGATCAGGTTAACAAGTGGTTAAATAATAAATCAGAATTAAACTGTGATGAAACAGTTATCAACAGCTTAAAAGCAGCTTTTACACTAAGTGATCTAATTCAAGCGCAACGTCAAAAACGCGGTACAATTGATCTTTCACACAAAGAAACTGAGATAGTTGTTGATGAACATTATTTTCCCATTAAGATAAATTTTTTGGTTCACGATAAAGCTGAAACCATGATTGAAAATCTCATGGTAGTGGCCAATGAGACAGTTGCTTGGGTGTTAACTAACAACAAAATTGCTTTACCATACAGAGTTCACCCAAGACCAAGCAAAAAGAAGTTACAAAGTTTGATTGAAACAGTTGGTGAGTTGAACATAACTAAACCCCAATTTAACTTAGATACTGTCACTTCAAGCCAAATAGCTAGCTGATTAAATGAAAACAAAGATAATCCTAGTTATGAGATCTTTGTAATCCTCTTATTAAGAACACTAGGCAAAGCTTTTTATAGTGTTAATCCCCTGATGCACTTCAGCATTGGTTCTAACCACTATACCCACTTTACTTCACCGATTAGAAGGTATATAGATCTAACCATTCACAGGTTGTTGTGAATGCATCTTTTTACTCCCGATCAATTCACTGATAATGAAAGAGATCAACTCAAACAAGAGTTGGAAAAAATTGCTGATACAGTTAATGATACAGAGATTAAAATTATCAATTGTGAAAGAAATGCCAATGATTATCTAACAACGCTGTTATTATCAAAACAAATTGGCAAAACCTTCAGCGGATTTATTTCAGCAATTACTAGCTTTGGAATTTTTATGAGAATGGATGAAAATAACTTTGATGGGTTAATCAAAATTACAACTATCCCTGATGATTTCTTTATTTTTGAAAAGGAAAAAATGGTATTGAAAGGAAGAAAAACTAATAAGGTTTATAAAATTGGCGATCGTTTGGAAGCTAAACTAAGTGAGATTGATTTCATCCAAAAACGTGCTATTTTAACACTCATATAATTAGCCAATGGTTGTTAATATCCTGCTTTTTATTACCCTTATTTTTTTACTTTTACTGTTTGTTTTTTTAATTGCTTTTGCATTTCTAAACAAACGGGTTAGAAATTATGTTGTACGTACTTGAACTAGTGTTTTCAGTAAGTCCAAACAAAACCTTGATAAAAAGAACTTTTTTGACAATTTAACTTCAACTTTATTAAGACTTTCAGTTGATAAAATTGGGGCTATTATTGCCATTGAAAAACGTGATTCACTTGATCCTTATATCAACATTGGTTATCGGGTTAGTTCTGATTTTTCCCCTGAACTGTTAGTTACTATTTTTTACAACAAATCATCTCCTTTACATGATGGAGCGGTGATTGTTAGAGATTACAAAATTATTAGTGTCTCTTCTTACTTTCCAATGACAAGACAATTAATTGATGTTTCATATGGTTCAAGACACAGAAGTGCCTTGGGATTAAGTGAAAAGTCTGATGCAGTTGTTTTTATTGTTAGTGAAACAACTGGTAAAATATCAGTTGCTTTAAAAGGGGTTATTAAAACCTTGTCTTCCAACTCAGATAGGTTGCAAGATGAAATTATCCATTACTTGTCTTCAAAGTAGGATTAATCACTTTTCATTCTGGTTTTTTAAACCATGGATTCAAAGGGTTAATTCTTTGGTAAAAAAAGCGTCCCTGTAAGTGATCAAATTCATGCTGTAAACACATTCCAAATAGTCCAGTTGCTTTAATGGTAATCTCTTTTTTTTCAAACCAATCATAACCTTTAATAGTGATCCATTCACTACGAATTACATAACCTTTGTGCTGCTTTTTAACACTTAAACACCCCTCACCACTTTCTAAAAATGCTTTATTTTCACTTTGATCAATAATTTTAGGATTGATCAGTAGGCACTTTTTTTCTTTGTTTAAATCATTGAGGTGGATGTAAAACAGTTGTTTTCAATAGCCAATCTGGTTAGCAGCTATCCCAATTCCTGGAATAATGTCATATTCTTGTGCTTTACCATCATATGAAGCATCAACATATGCAATCATCTTTTTAATACAGGTTTCAATTTGCTCATCTATTGGAAAATTAACAGCTTCAGTTGGCTTGTTAATCAAAGCATTATCATCAAAAACAAGCCAAGTTTTAGTTGGTTGAAAAGTCATTGATAAAAACAGTAAAGTTAAAATTATTCTAACAATTGAAGTGAACAATCAGGGGAGAATTTTTGTCATTACTGGTCCTAGCGGTGTTGGCAAAAGCACCCTTGTTAAAGCCTTATTAGATCATTTCAAAGAACAACTGTTCTACAGTATCTCTGCAACTACAAGAAAAAAGCGCATTAGTGAAAAAGAGGGAATTGATTATTTTTTTAAAGATAAAGATGAGTTTGAAAACTTAATAAAACAAGATGCTTTCATTGAATGGGCTTGCTATAATAACCATTATTATGGAACGCTCAAGTCTCAAGCTGAACAAGCAATTAAAAGCGGAATTAATTTAATGCTTGAAATTGAGTATCAAGGTGCTTTACAGGTTAAAAGTAAATATCCTCATAACGTTGTTTTAATTTTCATTAAACCACCTTCAATGCAAGAGTTGTTAAAACGTTTAAAAAAGCGTAATGATGAAGATGAAACCACAATTAAAAAACGTTTAGAACAAGCTAAGATAGAGTTTCAACAGATTGATAATTTTAAGTATGTTGTCACTAACAAAGAGTTTGATAAAACCCTTAATGAGTTGAAATCAATCTTACTATCTGAGTTTATTTAATGCAAGCTGCTAATGATCATTTCTTTACCGGTTTATCAAAAAAAGGTCCTGTAAGAAAGGAAAATCAGGACTTTTATGGATTTAGTTTTAATCAAAATAACCTACTAATAGTTGTTTGTGATGGTCTTGGTGGTTATAAGGGTGGCAAGATTGCTAGTAACTTAGTAGGTAAACTCTTTCTTAGTTTATTTGAAGGTTTTGAATTTAACCAGTGAGATGAAACTACAGTTAAAAAGTGGTTTGAAAATACTTTAATACAAGCACGCTTTCAACTAGAAAATTGTTTTCAAACAGTTTATGAAGCACAAATCCAGTTTGCTAGGATGGCTAGCACCTTGGTCTTGGGAATTTTAACTAAAAGTGATATTTATATCTTTTGAATTGGTGATTCCAGAGCTTACTTATTATTTGAAAACCAAGCTAAGTTAGTTACTAAGGATCACAATCTTTATAACCAGTTAGTTGCTATGAATGCTGATGAGAAATTACTTTTAAGTTATTCTAATCAACTTTTAGCATTAACAAATACTATTTCCAAAGAAACAAAAAGACCATTAGTTTATGGTTTTTATAACACAAAAATTGAACAACAAGAATTTTTATTATTGTGTTCTGATGGACTTTATAACTTTGTTGAAAAAGAGCTTTTTTTTGAAATTATCACTAATTCTAAAAACCTAAAACAAGCAGTGTTTAATCTTTATAGAAAAAGTATTGAAAATGCAAGTAATGATAATATTACTGCTGCCCTTGTTAATCTGCAAAAATGGAAGCAATCTTAAAGATAGGTGATATTGTTGAAAATAAGTATCAAATTGAAAAGCTTTTAAATAGGGGTGGGATGGATTCCTATCTTTTTTTAGCAAAAAATTTGAACTTAAAAAACTATGGACCAGTTCAAAAAAAACAGTATGGACACTTAGTTTTAAAGGTGGTTCAAAAAAATCCTAAGATTAATGAAAATAATTGGAAAAAGTTCCTTGATGAAATGGTAACCACAACTAGAGTGCACCATTCTAACCTAGTGAAAAGTTTTGATGTGGTGAACCCTTTTTTGAAAATAGTTCGTGGTAATAAAACAATTGCTCTCAATCAGATAGTGATGATTGCAATGGAGTATGTTGATGGCCCTTCTTTAAGACAATTGCTTAACAGAAAAGGCTACTTTAGTGTTAGCGAAGTAGTTTATTACTTTACAAAGATAGTAAAAGCCATTGATTATCTCCACAGTTTCAAGCACCAAATTATCCACCGTGATCTTAAACCTGAAAACATCTTATTTACTAGTGATTTAACTGATATTAAACTATTAGATTTTGGCATTGCTTCAACAGTAGTTAAAGTTGCTGAAAAAACCGAAGTTTTAACTGATGAAAATTCACTGTTTGGCACAGTTAGTTATATGATTCCTGATGTTTTGGAAAGCACAGTTAATAAAGCTGGTAAAAAGGTTAGAAAACCACCCAATGCCCAGTATGATATCTACTCGTTAGGAATTATTCTATTTGAGATGTTAGTTGGTAGAGTTCCATTTAACAAGTCAATTAACCCCAACAAGGAAAGAGAAACAATCCAAAAAGCACGCAATTTTGACTTACCTTTAATGCAAGCAACTAGAAGTGATATCCCAAATAGCTTAGAAAATATTGCTTTTCGTTGTACTGCTGTGAAAAGAGAAAACAATAAGTGACTTTACAGTTCCACAAAAGAACTTTTAGAAGATTTGGCGAATTGAGAAAATGAACAAGCGATGATTAAACCTGCTAATGAAAGGGTTTTAGAAGGGCAGGTGGAAATTAGAGAAATGATGCTTGAAAAGCCCTTAGCTTGGTATTTCAAAACTTGAGCCTTATCAATCTTTACTATCGTTTTTATAGGGTTAATTATTGCTGCAATAGTTCTTTTATTAATTTTTAATGCCAGATTCTAACTTTGGGATAGTGCTTCAGTCATTAGCAAAACAATGCAAAGTATTTTGGAATAACCAGATAATAACTGCTTTTCCTCAAAAGAAGTTACAGTGAAAAAATGATTTTAAGTTGATGGTTGGTGATAGGGTTCAACTTGAAGATGGTGCGATCACTAAAGTTTTAGCTAGAAAAAATGAACTTACAAGACCAAGGGTTGCTAATGTTGATCAGATTGTTTTAATCCAATCACTAGTTCAACCTAAGATTAACTGAATTCAACTGTTGAAATTATTGGTATATTTCAATGCTAAATTAATTGATGAAATACCTATTTTAATAACAAAAACAGACCTTGATTTTGATCCAATGGAAAAACAAAAGTTAATCGATTTAAAACAGTTTAACTATCAGTTATTTTTTGTTTCTAAAAATGAACCACTACCTTCAGAATTAATCGATATTTTTAGTAAAAAACTAAGTGTTTTTACAGGTCAATCTGGTGTTGGTAAATCTAGCTTAATTAATCGTTTAGATCCTTCTTTAAAACAAAAGATTCAAGCCTTATCAGTTAATAAATTTGGTAAGAATACCACTACTAAAACAACACTTTTTTCATTTAGAGGCGGTTTTATTTGTGACACCCCTGGTTTTAATGTAATTTCTATTAAAAACCTCAAAATTTTAGCAGCCCAACACTTTGTTGGTTTTCAGAAAATGATTAGTAAGTGTCATTTTTCTAACTGTTATCATCAGTATGAAAAAGATTGCTTTGTAACCACAAGTGTTATGAAAAACAGATATCCTTCGTGATTGTATGAGAAGTATAGAAAAATGATTAATTAAAATCAACTGCAAAAGTGTTTATGAGTGATAAATTATTAACAATTGACTTAAGTCATGTTTATGGATTTGATAAAGAAATTATTTTTAAGAAATACCAAAAAAAAGTAGATCAAATTCACCAAGATTTTCTAGCTCATAAACTTGCTGATGGTCACATGACTGGGTGGTATGACCAACCTGATCAAAACCACCAATTCCTTTTAAAAACCATTAATCAAATTGACAAAAAGTTTAAAAGTTTAAAAGTAACTGACATTGTTTATGTTGGTATTGGTGGTTCTTTTACTGGTATTAAAACAGTTTTAGATTTCTTAAAACCAAAACAAAGAACAGGATTAAAAATCCACTTTGTCCCTGACCTTTCTGCTTTTCAAGCTGCAAGTGTTATTAAGGAAATTAAAAATAAATCATGGGCTCTAATTACCACTTCTAAGTCTGGTAGAACCCTAGAACCAGCACTGAATTTCCGCATTTTTAGAAACTTATTAAACAAGCGTTATGGCAACAAACACTACCAAAGAGTAGTTGTTATTACTGATGAAAAAAAGGGATTACTAACCAAAATGGCATCAAATCATGGTTACCAAAAGTTAGTTATTGATTCAAATATCGGTGGGCGTTTTTCAACTCTATCTCCTGCTGGTTTGTTACTAGCCAAACTTTTTGGTCATGATCCTAAGGCCATCTTAAAAGGAACATTACAAGCCAAAAAGGATTTGCAAACAACTTCACTTGAAAACAATTCTGCATACCTTTATGCAGTAGTTAGACATTGACTATACACCACAAAAAAATTCAAAATTGAAGTTTGCATTGCTTATCACAGTTTGTATGAATATTTGTTATTACAGCATCGACAACTTTTTGGTGAATCAGAAGGTAAGAACGATAAATCTTTATTTCCTACTTTTTCGATTTTTACTGTTGACTTACACTCAATGGGACAACTCTATCAAGAAGGGGAAAAAGTGTTTTTTGAAACAGTAATTGATGTTAAAAATCCACTTGTTAATATTAATTTACCTCCATCTGATTTTGACAATGATGATGAACTTGATTTCTTGTTAGATAAAAGCTTAAATGAGATTTCAGATGTTGCAATTGATTCAGTTATTAAAGCGCACTACCAAGCAAATGTAAGCATTATTAAATTAACTTTAAAAGAACAATCTGCATTTATGTTTGGTTATTTTTACTTTTGACTCTCTGTTGCTACAGTGATGAGTGGATCATTATTAGGGCATAATGTCTTTAATCAACCTGGCGTTGAAGTTTATAAAAAGTTAATGTTTGAAAAACTAAGAAGTGGCCACTAAGGTTGTTTTTTCACTCTTACCACTTTTAAATAGGTTTGACAAGTCACTTTTAGAAAGTTACTTTCAAGATGGATTGAGGTTAATCCATTATGATGTGATGGACCAATTTGTTCATAATACTGCTTTTAAAGGTGAATATTTGGATGAATTGAAAACAATAGGTTTTGATGTTAATGTCCATTTAATGGTGGAACAGATCATCCCTCAAATAAATTTTTATCTTTCACAACCTAATGTGAAAAGGATTTCGTTTCATGTTGAACCATTTAGTTTTGCAAAGATTAAAGAACTAATCCAACTAGTTAAAGAAAATGGTAAAGAAGTTGGTCTTGCTTTTAAATTTACAACCAATTTACAACTATACCAACCATTTTTTACAACCATCGACTTTATCACTTTAATGAGTGTTCCTCCTGGTAAAGGTGGTCAAGCTTTTAACGAAGCTGTTTTTACAAATTTAAAGATTGCTAACCATTACAACTTGAAAATTGAGATTGATGGTGGGATTAAAGTTAATAACATTGATCAAATTAAAGCCTTTGTTGATTTCATTGTAATGGGAAGTGGCTTTATAAAATTAGAGCAGTGGCAACGTCAAAAATTGTTGCAAACAATCTAATTAAACTTTATTGATGAAATCAGTTACAGTCAAGCAGTTACTACAAACCCCACGAAAATTTAATAACAAGCAGATTAAACTATCAGGTTGGGTTAAAAATAAACGTGCTAGTGCTAACATCATCTTTCTAGCAATTAGTGATGGCTCTAGTATTAATACCCTACAAGCAGTAGTAAAACAAGAAGATAACCCCCAGGTTTTCTCACTGTTACAAACTGTTAATTTAGCAAGTGCTGTTATGGTTTGAGGGGAAATTATCTTAACCCCAAAAGCTAAACAACCACTGGAGTTGAAATTAAAGCAGGTGAGTTTATTAGCACAAGCAGAGTCTGATTATCCACTGCAAAAAAAAGAACATAGTCAAGAGTTTTTTAGAAGTAATGCGCATCTAAGAGTAAGAGCAAAAACTTACTTTGCAGTGATGAAAATAAGGAGTGTTTTGTCACACGCAATCTTTGAATACTTCTTTAAAAATGATTTTATCTTAGTGCAAAGCCCTATTTTAACTAGTAATGATTGTGAGGGAGCGGGGGAAACATTTGTAATTAAAGATAGTGAAACTTTTTTTAATAAAACGACTTTTTTAACAGTAAGTGGCCAGTTTGGAGCAGAAGCTTTTGCGCAAGCATTTAAAAAGGTTTTCACCTTTGGTCCTACTTTCAGAGCTGAAAAATCCCATACTAATCGTCATCTTAGTGAGTTTTGGATGATCGAACCTGAAATTGCATTTGCTAACTTAAAAGATTTAATGCAGTTAATACAAAACCTAATTAAATTCTTAATTAAAAAAGTGATGGAAAATGCTAGTGATGAACTAAATGTTTTAGCAAAGCAATTTAGCAATGACATTATTAGCAACTTAAAGACAATCATTAGTACTAAAAAATTTCCAATCATTGAATACAGCAAAGCATTAGCGATTCTAAAGGAATCTAGTGATACAAAAAAAACTAATTTTGAACTAAACGACTTTAGTTTTGGTATTGACTTAAAAACAGAACATGAACGCTTTTTGTGCGAACAATATTTTCAAAATCAACCGCTTTTTGTTATTAACTATCCAAAGGAGTTAAAGGCATTTTACATGAAAACAAATACTGACAATAAAACTGTTGCTGCAGTTGATCTTTTATTACCAAAGATTGGTGAGATTTGTGGGGGAAGTGAAAGGGAAAGTGATTTAAACCAACTTAAGAATAGGTGTCAATCTTTAAACATTGACACAAAAAGTTTGAACTGATATCTTGATATGAGGAAATGGGGTTATTTTGCTAGTGCAGGTTTTGGTTTGGGCTTTGATAGATTATTAGCTTATATATGTGGATTGGAAAACATCAGAGATGCTATTCCCTTTCCCCGTGTACATGGCACCATTAACTTCTAAATTCGCTGCTTATAAAAAAAAGATTGCAAACTGGTTAACAGTTTACAGAATTTTTATTGCTTTACCTACTATTATTTTTATTGCTTTAGATAATCAACTAGGAGTTTTAGCTAACTTTTCTGTTGGTGCAATTAGCATTAGTTTACAGATCAGTTTATTGATTGGAGGATTTTTGTTTTTAACTGCAGTTATATCAGATTATTTAGATGGATATTTAGCAAGAAAATGGCTAGCAGTTTCTAACTTTGGTAAATTATGAGACCCCATTGCTGATAAAGTGATTATCAATGGTGTTCTTATTGCACTAGCGATTAATGGATATTTTCACTTTAGCTTATTAATTGTTTTTATAGTCCGTGATCTTGTGTTGGATGGAATGCGGATTTATGCTTATGAGAAAAAGGTGGTTATTGCTGCTAACTGACTTGGAAAATGAAAAACTATCATGCAGATGGTTGGTATTGTTTTTAGTTGTTTTGTTTGGAGTTTTAAACAAAGTGAAATAGCTTCTTTGAATAGTGGACTGTTCTTTTGATTACTAACTCAACTGCCATATTATTTAGCAGCAGTTTTTTCAATTTGGTCTTTCATTGTTTATAACATCCAAATATATCAGCAACTAAAGGCTTATAACTCCAAGTTATAATCCTTATTAGGCATTGGAATAACTTTGTTTGCCAATTTAATAGCAGAAAAACTCCAAAAGTTACAACTTAGTGTTGCAACAGCTGAATCAGTTACTGGTGGCTTATTAGCTCATTGTTTAACTTCCATTGATGGTGCTTCTAACTATTTTAATGGCGGTGTTATTGCTTACAATAACCAAGTTAAGATTAACTTACTGAATGTTCAATCCTCCACAATTGCAAACCATGGGGCAGTTTCCAGTTTCTGTGCTAGGGAGATGGCAGTTGGGGTTAAACAAAAGTTTCAAGCTGATGTGGGTATTGCTTGCAGTGGGATAGCAGGTAGTAAAGCAGTTGAAAATAAAGCAATAGGATTACTTTTTTTCTGTATTATTATAGGAAATAAGGCTTATGATTTTGAGTTTGAAATGAACCAAAATAATCGTAAGGATAACATTGAATTATTTACCAATAAGATCTTGGAATCTTTCCACTATTTGTTAACAAAGCTAGCTTAATCTAAGTTATGAAACGTACTTTAAATATAGGTATTGTTTTGTGTGAAAATTTTCTTTCAGACCAACAAAATGCTGTTGATAGTTACACCCAAGTTTATGAAGATGTTAGGATGTTTGAATTTGGGTTAAAACTCTTTCAATCATTACCTTTTAACATCCAAGAAACCCTTATATTTTGTAATGCTGAACAACATAAAATAGTTGATAAAGCTGCTAAAAAATACAAAAACACTACTGTGTTTTTTTCACGTGATACTGATGTAGCTAATGTTTATGAAGCTAAGGTATTTATCCAAGAAAAATACAAATTAACCCAAGATTACAAAAAAAGAGGTGTTAGCTCTTATTATGACAGCTGTTGCTTTATCTTAATTGAAGCGAATCGTCCATTAACTGCAATAAAGACAGTTAAAAGTGTTTATGAAAAAGCACTGATAGAAAAAGCAGCTATTGCAGTATTACCATATAATGGTACACTAATGAATGGTAACAATGATGTTGTCTTTAGCCACTTGCAAGATAAAAATAGGTTTAACTATTGAAAACAAAGCAAGGCTTATGAAGTACAATACCCCCAAGCATATACTTTAAATAAACTTAATCAGTTTTCAAAACAGCAATTTCTAAGGGCTAGAAGTATGTTGGACTTAATGAAGATTTCTAATAAATCACCCTTAAGTATTGTTGATGGTAGTGCTTATGCTTTTAGGGTTGTTACCAACCTTGATTTTGAAATTTTATTAGGTATTTTAAAAAATGGATAAAAATATTTTTCAATTAGTACAAAGTTTTGCTAAAACGCAAAATGTGCGAGCTAATTACACCTCACAAGACAAAAATATTAGTTTAGATTTTGTTAGTTTTGAAACAGTTAGTCAATCACTAACAGGTTTTCTAATTTTTAATAATTTCAATAAATTGTTACAACTAATTGAACTGATAAAACAAAAGCAAACATGATTATATGTAGATCAGCTCTGAATTGTTGATCTTTCCAATAACAAAGCCTTAACTGATGCTACTGTTTGGATTATTAAACAAGAAAAATTGCCTGTTTCAGTTGCGGCTTTTAGCAACCAACAACTCAACCAAGTTTATCGCAATTCATCCACAAGTCCTTTGTATCTTAGTTATGTCAAACCAATCGAAGTTCAACAATTTTTTACACTTTCTCCATCCATTAACAATAATCCTTTTTTAAATCAAAATCCTTTAACTGAATCACCATTTGATAACAATAATCAGCTGTTTCAAGCAACAAAGAGCGTTGAACCTTCAATGGAAACAATGGAATTTTCCCGGTTTATGGATGAATTTGATCAGATTACAAAGAACTTTTCTGATATAGAGCTTGAACCTATGCAATTCACCCAAAGTTTTGATGATTGGAGCAAAGACTAGGGTTGCAATAGTTGGCGGGATTGGTTACATAGGTAGTTGTTTTGCTAGTTTTATCAAAGAACAAAATGATAAGCTAATTGTTACTGTTATTGATAACAACAAAAATAACCATGTAATTAAACTCTTAAAAAAGATTGGAATTGAATTCTATTTTGCTGATTTACTAGATAGACATAAGCTAACTGAAGTAATTGCAGCAATTCAACCTGATGTGGTATTTCACTTTGCTGCTAAAACAAGTGTAAGTGAATCAGTACATAATCCATTGAAGTACTTTGATTGCAATGTAATTGGTACTTTAAACCTAATTAGTGCAATTAGTAACTTACAGAAGCCAATTAAATTATTTTTCGCTTCTAGTGCTGCAGTGTATGGTCAAACAACTAATAGTTACATTAGTGAAGAGATTGTAATAACTGAAACACAAGCAACCAATCCTTATGGATTGAGTAAGTTTTTAGATGAATTAATCTTAAATGCAGTTGCCAAAAATAGTCAACTACAAGTTGTTTGCTTACGCTTTTTTAATGTGGCAGGTGCAATTCTGCCATTTGGTAATTTTAATGGTAATACCACGCTTTTAATTCCTAACTTAGTAAAAGCCTTTTTAAAACAAACTCCCTTTTTTTTATATGGCAATGATTATGCAACTAAGGATGGTAGTTGCATAAGAGATTACATCCATGTTTATGATATATGTAATGCTCATTTCTTATTATGAAAGTGGTTAAATGATCATCGCCAAATTAAATTTGAAACCTTTAACTTGGGGAGTGGGATAGGAACTTCTAATTTAGAAGTTATTGATATTGCTAAAAAAGTGTTTTATCCTAGTAGATTAAATTTAGAAATTAGACCAAAAAGAAGCTGAGATCCAGCAATTTTAGTAGCAAATGTTGCTAAAGCAAAACAAACCTTTCAATTCAAAATAACGCGTAATTTGAAAGATATGATAAGTGATGAGCGTAATTTTTATGAGAATTTTTATAATGACGCTTATTAAGTGGTGTTTAATTAATGGAAAAAGTTGCCTTCAAAATGGAGCATATCTCCAAAAGTTTTGACAATGGCAAAATTAAGGCTAATGTTGATGTTAGCTTAGTTGTTTATGAAAATACTGTCCACACCATTTTGGGGGAGAATGGTGCAGGAAAATCAACCCTGACTTCGATTTTATTTGGTTTATATAAACCTGATAGTGGCAAGATCTTTATTGGTGAAAAGCAAGTAAATTTTAAATCTTCTAAAGATGCAGTAAAACATAAAATCGGAATGGTGCACCAGCACTTTAAGTTAATAGAAAACTACACGGTTTTAGATAACATCATTCTAGGGAATGAAAGTAGGTTTGGGTTTTTACCTTTAATTAATCGTAAAGTAAGTGAAGCAAAGATTAAAACCATCATGGAAAAATATGGAATCTTTGTTGATCTTAAACAAAAAGTTAGTAACTTAACAGTAGGTCAGCAACAACGGGTTGAGATCCTAAAGGTTTTATTTCGTGATAGTAATATCCTTATCTTTGATGAACCCACTGCAGTTTTAAGTGATCTTGAAATTCAAAACTTTCTCAAGATTATTGCTAACTTTAAAAAGCTAGGAAAAACAATTGTTTTAATCTCTCATAAATTAAATGAAATTAAACAAGTTGCTGATACAGCTACTGTCTTAAGACTTGGCAAGGTAGTTGGTAGTTTTGATGTTAAAACAACACCAGTTGATAAGATTGCGCTTTTAATGATGGGCAAAGAGTTAAAACAAACTAAAAACACCACAGATTTTGTTGCTAAAGATGAACCTGTTTTAAAAGTTCAAAACCTGAATTTGTTTCTCAATAAATCTTTAGCATACAAGTTCTTAGTGAGGTGCAATAACATCCATAAAGCCCAACAAATTAAGAAAAATAAACCATTAAAAGACTTATGGATAATTAGTTTTTTAAATAAACTAACCACCAGTAACAAAACCCCTAAATTAGTAAAAGGCTTGATTAATAAGTTAGGACTTTCCTATCAAGAAAATACAGATGAAACCATTAGTTTTGCTATCCATAAGGGAGAAATTTTTGCTATTGCTGGGGTTGAGGGTAATGGTCAAAGTCAGCTTGTTAATTTAATTTGTGGAATTGAAAAAGCTGCTAGTAATAAGTTAATTTTTAACAATATTGATATCTCAAGATGATCAATTAGAAAACGGATTAATGCTGGGATTAGTTTTGTTTTGGAAGATAGACATAAATATGGCTTGATCTTAGATCAAACCGTGAGGTTTAATACGGTTAATAACCAGATTAATAACCGTCCTTTTAGTAGTTGAAACTTTTTAAAACCAATGGAGATTGCTCTTTATAGCAACACTATTATTAAAAAGTTTGATGTTAGGGGCAGTGCTGAGGGTAGTGCTGTTGTAAGAAGACTTTCAGGTGGTAATCAACAGAAACTAATTATTGGTCGAGAAATGACCAAACAAAATGACCTTTTGGTGTTAGCACAAGTAACCAGAGGCCTTGATATTGGTGCTATTGCTTTTATCCATGAAAACATCTTATTAGCTAAAGCTAATAATAAAGCTATCTTATTGGTTTCATATGAACTTGATGAGATCTTAGCACTTGCTGATACAGTGGCTGTTATCAATAAGGGGAGAATAGTTGGTATGGGAAAAAGAGATTTAATGGATCGCCAATCGATAGGTAGATTAATAATGCAATAAAAGACTATGACAATGTGGCAATTTAAAAGTTACTTTAAACACCACCTGGTGTTTTGAAAAGACCGATTTTTACATAGCTCTGAGAAACAAATGCAAAGAAGAAGTATCCTCTCTTCAGTGGTTTTGATAATCCTCTCTTTTCTTATATCGTTTTTACTGATTATTTCAATTCCTGGAGGTAGAGGTGCGAGCTTCTTTGCACTGTTTACTAAGTTATTTTTAGATAACACTAATACTGAAAATTTCTTAAGACAGATTGCTATTTATATCCTAGCTGGATTAGCATTTAGTTTCTGTATGAGTGTTGGTATTTTCAACATTGGTATCTCAGGGCAGATGATGGCTGGAGCCATCTTTGGGTTTTTAATGATTCTCAAGGTGTTTCCAAGTTCATTTCGACCTGGTTTTGGAGGTCAGATTATTACTGTATTATTGATGGTAATAGGTAGTGTTAGTGTGGCAGTTGTTGTTGCAACTTTAAAGATTTTTTTCAAGGTTAATGAAGTTGTAAGTGCAATTATGTTGAACTGAATTGTAGTGCTTATTAGTGCTTATTTAGTAGAGACTTACATTAAAGATAATAGTGGGGGTACAGCCCAATTCTTTTCCTTACCACTCCCTGATGAATTTGCTTTATATAACTTCTCTCCTTTAACAAAAAAGTTTGGTTGATTAGCTTCACTTATTATTGCTTTCATTAGTGTTATTATTGTGGCAGTAGTATTAAAATACACAGTTTTTGGACACAAATTAAAGTCAATTGGCAGTAGTGTATTTGGTTCTCAGGCAATGGGTTTTAATGTTAGAAAATACCAGTTCTTATCGTTTATTATCTCAGGAATTTTATCAGGACTATTAGCAACGGTTGTTTACACTGCATCAACTGAAAAAGTATTGACATTTAACAATGTTGGGGATAGTGCTATTTCAGCAGTACCAGCTACTGGTTTTGATGGGATTGCGATTGGTTTAATTGCTTTAAATAACCCCTTTAGGATTGTTATTGTTTCTGTTCTTATTGCTTTTGTTAACATTGGGGCAAGACCTGCTAATTTAAACCCTAATACTGCTAGTTTAGTTTTAGGAATCATGATGTATTTTGCTGCACTTTATAACCTAATGGTTTACTTTAAACCATGAAGATACCTAGTGAAGCTGAACATTGGAAAGATAAATCTCACCACATATGAAACATATGAAAACAAACTAGCTGCTAACCTAGAGTGACTAAGTTTCCAACGCTTCTTGTCAAAACAGAAAAAAAAGAATGACAAAACTAAATTTAATTGGTTTGATACTAGTTTATTTGAACAATATGCAAAAAACAAACAAGAAATTGTTCAAGAATACCATCACAATTGTGCAACTAATTTAATTGCTTGGTGATTGAATGCAATCCAAAGTGGCAATATTAAACCTTCAACTACTTTTAAGTTGGAATTTGTTAATTTTAAACACCAACAGAAGTTTGTATTAAATTGGTTTAAAAATGAAAGTGAATCACTGCGTGATTTCCAATCACAGTTTGAGAGAATCAATAAGTTAGTGGAAAGGGAGTTTGTTAAGTAACAATGTTAAGTTTAGCACAATTAGAAAGTTGGTTTTTTATCGCTCCAGCACTGCTTTTAGCAGTATTGAGTGGTTATCTCGCTGAACGCGTTGGGATCATTAATATTGCTATTAATGGTGGAATGGTGTTTGGTGGGTTATTTATGGCACTCTTATCGTATGGATTTACAAATAACTTAAATCAATCCGCTCCCTCCTGATCACTATTTATCACCATTCCCTTATCAGTTTTATTTAGTAGTGTTATAGGTTGTTTATTTGCACTAGCAGCAGTTAAGTTAAGAGCAGATCATGTTATTGTGGGAACTGGGATTAACTTGTTGGCTAGCGGAATTACCCTTTTTATTAGTCAGAATGCTGCTAGTTTGTTTTCCGATACTACCTTAAGAGTAAGGTACTTATTTCCCATCCAAACTACTGTTAGTATAGAAGCAATTGGTGTGTTTGTTTTTAGTTTACTTCTGATTGGGTTTGTATGGTACTTGATGAGTTTTACTAAAACTGGGTTGAGATACCGTGCGGTAGGTGAGAATCCTAATGTAATTGATACCCAAGGGATTAGTGTTTACAAATACCAATGGATAGGCGCAATTTGTTCAATGATGGTAGCTGGATTGAGTGGTAGTTTGTTTGTTTTAAGTGTTTCTAACTTTCCCTTTAACAGCGGAGATGTAAATGGCTTGGGTTTTATTGCTATTGCCATTATGATTATCTCAATGTGAAGAATTATCCCTAGCATCTTTATTGGGTTAATCTTTGCATATGCCTATGTTTTTACCAATAGTCAAATAGGATCTAATAGTAATTCCTACTTGTTAAGAACGATCCCTTTCATCATCTCATTACTAGTAATGTTGTTATTTGGTTTTCTTAATGTTGCCCCAAAAAATATAGGTAAACATTTTGACAAGGGTTTAAGATAAACAAAAACCTTATTTATAGTTAAGTAAGTAGTTTTATTAATGATTAAAAACCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACATTAAAACAATATCTTCCTAGTGATGAATTTGAGATAGTCTCAACCGTTGGTCACATCAGAGAAATGGTGTATAAAAACTTTGGTTTTGATGAAAATACCTATACCCCTATCTGAGAAGATTGAACTAAAAATAAACAGAAAAATCCCAAACAGAAACACCTGCTCAGTAAGTTTGAGATCATCAAATCAATCAAAGCTAAAGCTAGTGATGCACAAAACATTTTTTTAGCTTCTGACCCTGATAGAGAAGGGGAAGCCATCTCTTGGCATGTCTATGATTTATTGGATCAAAAAGATAAAGCTAAGTGCAAACGAATCACTTTCAATGAGATCACTAAAAAAGCAGTAGTAGATGCATTAAAACAACCGCGTAACATCGATCTTAACTGGGTTGAAAGTCAGTTTGCCCGCCAAATCCTTGACAGGATGATAGGTTTTAGATTATCAAGATTATTAAATAGTTATCTGCAAGCAAAGTCTGCAGGTAGAGTTCAATCAGTGGCTTTGCGCTTTCTTGAGGAAAGAGAAAAGGAGATAGCTAAGTTTGTTCCGCGTTTTTGGTGGACAGTTGATGTTTTATTAAACAAAGAAAATAACCAAAAAGTAGTTTGTGCAAACAAGTCTATTCCTTTGGTTTTAAGAGAAATTAACCCTGAATTAAGTGCTAGTTTAAAACTGGATTTTGAAGCTGCTGAAAACGTATCAGGAATTGACTTTTTAAATGAAGCTTCAGCAACCAGATTTGCCAACCAACTGACTGGCGAATATGAAGTTTATTTTATTGATGAACCTAAGATTTACTATTCATCTCCAAACCCAGTTTATACCACCGCTTCACTTCAAAAGGATGCAATTAATAAGTTAGGATGGTCTTCCAAAAAAGTAACAATGGTGGCCCAAAGACTGTATGAAGGGATTAGTGTTAATGGGAAACAAACTGCATTAATTAGTTATCCAAGAACTGATTCAATTAGGATTTCAAACCAATTTCAATCAGAGTGTGAAAAGTACATTGAAAAGGAGTTTGGAAGTCATTATTTAGCTGATAAAAATAAGTTAAAAAGACATAAAAAGGATGAGAAAATCATCCAAGATGCCCATGAAGGGATCCATCCTACTTACATTACTATTACCCCCAATGATCTGAAAAACGGGGTGAAACGCGATGAGTTTCTCCTTTATCGTTTAATATGGATTAGAACAGTTGCTAGTTTAATGGCAGATGCTAAAACATCAAGAACTATTGTTCGTTTTATAAACCAAAAAAACAAGTTTTATACCTCTTCAAAATCACTTTTATTTGATGGTTATCAAAGGTTATATGAAGAGATTAAACCTAATACTAAAGATGAACTTTACATTGATCTTAGTAAGCTTAAAATTGGTGATAAATTTAGTTTTGAAAAGATCAGCGTTAATGAGCATAAAACCAACCCACCACCACGTTACACCCAAGCTAGTTTAATTGAAGAGCTTGAAAAATCTAACATCGGTCGTCCCTCTACTTATAACACTATGGCCAGTGTTAATCTAGAAAGGGGCTATGCTAACTTAGTGAACCGATTTTTTTATATCACTGAGCTTGGTGAAAAAGTTAATAATGAACTTTCCAAGCATTTTGGGAATGTAATTAATAAAGAATTTACCAAGAAGATGGAAAAATCTTTGGATGAAATTGCTGAAAACAAAGTAAACTATCAAGAATTTCTTAAGCAGTTTTGAACAAATTTTAAATCTGATGTTAAACTAGCTGAAAATTCAATTCAAAAAGTGAAAAAGGAAAAAGAATTGGTTGAAAGAGATTGTCCTAAATGTAATCAACCGTTGGTATATCGTTACACCAAAAGAGGTAATGAGAAGTTTGTTGGTTGTAGTGATTTTCCTAAGTGTAAATACAGTGAGTTTAGTAATCCTAAACCAAAACTAACCTTGGAAACACTTGATGAATTGTGTCCTGAGTGTAACAATAAACTGGTTAAGAGGAGAACTAAATTTAACGCTAAAAAGACCTTTATAGGTTGCAGTAATTTCCCTAACTGCCGTTTTATCAAAAAGGATAATGCTGCTGAATTTAAACAATAACAGCGTTCTTATTGTTGCGTTTGTAATTGTTTCTTTATTCTTTCTAATAATTGTTGGGTTTGCTTTAAATTTAGCAATTGCTTTTTCACTCCATTTAAAGCAGAATAAAAATAACAAAAAATACATCTTAAATGACCAACAGATCCAGTTAAGATTAACTGAAAAACAAGCCCAATTAACAACTTTACTTAACTTTTATCAACAAAAAATTGAAAGTGTAAACAGAGAAAAAAGTTGGTTAGAAAGTCAGTTACAGGTAATTGATAAAAAGGATTTAAAGCAAGCGCAAAAGTTAACTTTACATTTAAAAAAAGACCAAATATTAGCTCAACTTAATGAAAAGCTGATTCAAAAAAAAGTTGATCAGCCTTTAGTTAATGAACTACAGAAAACCAAACTTTCCTATCTTGAAAGGTTAGTTGATCAAAAGATTAAACTCAGTGAAAATAATTTCAAAAGTGCTTTTCTTAAAACGAAGGTAAAAGAGACAGCATTTAATATCTTTGCAGCTAAAAACAAGGTGAACTGGGAGTATTTTAAACAGGTGTGTGATGCTGATTGCACTTTAAAAAACTTAGAAGATGAAGTGGAAATTACTTTTTCTAATTGGAGTTATTTGAGAAGGATGCAAGCTCTATTAGCGTTTGAAAAACTAATTAGCAAAATCAAAACAGTCAAAATTAATGAATTGGTTATTAATGAAACTTTAGATGAAGTGAAAAACGAAATTAGCCAAACTGCTTTTCAAGCGGGTGAAAAAATAGTTAAGGAATTTCAGATAACTAATTTAAACGAGCAAATTACCAGACTAATTGGCTTGCAAAAATACTATTTTGGCACTGATCAGCTCAATCTCTTAGAACTCGCGGTATTAACAACCAAATTAGTAATTTTACTGAACAAAAAGTTCAAACTAGATTTAGATTTAGAACTTCTTAAAGCAGCTAGCTTGTTTAACTATTTAAAGTGAGTAGATAATAACCAGTTCTTTCAAATATTAAACACAAAACTTAACCAGCTTTTAATTAGTGATCAAGTTATTGCAATCATCCAACAACAAGAACTTAGTTTTTATCCTGATCAGTATGGGATGTTAATTAATGGAGTGAAAACAATGATTAGAGAGCATAACACCATTGATTTTGAAAAACTGGTTTTTCTCAACTCAACAAAACTTATTGATAATTTTTATCTCTATGATTTAAATATGATCCATGCAGTTGAATATAACAACTGCTTTTATTACTTTGTTAGTGTTAAACCCTTTGAGATTAAGTCATTAGCAGAACTTGATCTTTTTGTTGTTTTGTTAAAAACTTTTTTAGCTAAAAAGCAAAAGCAAAATCCAAAAGCGGTTAAACTGTTTATCACTACCAAAATTTTAGCGATCTAATTACATTAGATTTTATTAGAATTGTTTCAACTAATAAATTGGCCTTATGGTAACAGAAATTAGAAGTCTTAAACAACTTGAAGAGATCTTTTCAGCTAAGAAAAATGTTATTGTTGACTTTTGAGCAGCATGATGTGGTCCTTGTAAACTAACCAGCCCTGAGTTTCAAAAAGCAGCAGATGAATTTAGTGATGCTCAGTTTGTTAAGGTTAATGTTGATGATCATACTGATATAGCAGCAGCTTATAACATTACCTCTTTACCAACTATTGTTGTTTTTGAAAACGGGGTTGAAAAAAAGAGAGCCATTGGCTTTATGCCAAAAACCAAAATTATTGATCTTTTCAATAACTAAAGTCCATGATTGATCTGCTTGGTTTGGATCTGGATGGAACGTTATTATCTAAAACTAAAAAAATTAACAATCCATCAAAATTAGCATTAACTAATTTAATTGCTAAAAAACCAAGTTTAAAGGTGATGATTTTAACTGGTAGATCAGTTTTTTCTACTCTAAAACACGTTGAAAAGCTGAACAGTTTGTTTAAAAAACCAATTGTTGATTATTTTTGTTGTTATGGGGGTGCTAAACTTTATCAAATTGAAGCAAATAAGCCACAAGAAAGATACAAGTTTTGCTTGGAAAACAGTGTTGTTGAAACTACCTTTAGTATTATCAAAAAACACCGCGGATTATGTTTAGCTTACTTAGATAGTTATGTCTCTCCTTACCTTTGTTTAGCTGGTAACAAGCTCCTTGGGTGGTTCACTAAATACTTTTGGTATAGAAAAAGGTGTGTGTTTTTTAACCAGAACCATTTAAAACAAGGTATTCTAAAGATTAGTGTTTACTTTTTAAGTGCAAAAAGGTGTAAAAAAGTTTATGAAATCTTAAAAAATACCTTTCAAGAAAAGGTTAATGTTTTAAGTTTTTCTAATAATTTAATTGAGATAACTCATCATGATGCTAATAAGGGTTATGCAATTGAATATATGGCCAAAAGAGAACAACTTTCACTTAATAGAATAGCAGTTATTGGTGATTCTTGAAATGATTATGCAATGTTCAAAAAAGCTAAATATTCCTTTGCAATGTCAAAATCCCCTTCCCAGTTAAAATTAATTGCTACCAATACCAGTAACAAAACCAACCGTTACCGCTTTAGTACCTTACTTAATTTAATTAGTGAAACAATCATTAATCAAAAAGCTGATTAGATGTTAAACCAAATGCTTTATAAAACATTTTTAATGTTTCATCAGCAACTTTTTTAGCTTGGTTTTTACCATCATTTAGGACTTTTAATACCATTTCATCAGTAATTTGTGCTTTTTTAAATTTAAGTGATTCTATGACATTAATAACAGCACTACTGAGGTCATTCTTTAAATCTAAATAACTTTTATTCTGATAATATTTAACAAGATCAGAGCCGATTTTTTTACTTAAATTATGGTTAACTTCTTCTTTTAAAAGTGCAGTTAAAATAACAAGTAAATTAGTAACACCAGGTTGGGTTTTTTTAGCAAAACGAATCTTATTAAAACTATCAGTTGTGGCTTTGCGCACTTTTTTGATGATTGTTTCTTTACTATCATCCAGATAGATAACACCATTTTGATCAGGATTTGATTTGGACATCTTTTTTAAAGGATTTGATAGATCCATGATCCTGTTGGTATCTTTGTTTTCTATAAATACAGGTAATTTCAGTTTTAACTTAAATTTTTTTGCTACACGTTTAGCTAAATCATTGGTTAATTCCAAGTGCTGCTTCTGATCATTACCAACTGGAACAATATCAGGTTGATAAAGCAAGATATCAGCAGCCATTAACACTGGGTAAGTTAACAAACCAGTTGGGATAGTAATGGTGTTATTACTATTTCTTTTTTGCGCTAATTTCTTTGTTTTAAATTGGGTCATTCTTTGTAATTCACCTAGATTACTTTGTGTCAGCATTAGATAACCTAACATGGTATGTTCCATCAGATCACTTTGTAAAAATAAGTTCACTTTTCCATAATCAAGTCCTAGTGCTAATAAAGTTTTAACAAGTTGCAAGTTGTTATCTTTGAGCATTGTTGGTTCAAAATCAACAGTAATAGCATGAAGATCAGCAACAAATAAAAACAGTTGGTATTGACTTTGGAGTTGTTTTAAACCTTGCATTACGCCAAGAAAGTTACCTAGGTGTTGTCTTCCAGAAGCTTGAATCCCTGTAATTGCGCGCTTTATCATTATTAGAATTGATAGGGTAATTAAAGGTTATTTTAATGAAAAAAGCGGGTAAAAAAAATAGTGATGCAGGTAAGACTTTTATCTTAATCTCATCATCCTGTTCATCCTGTCAGAAAGCCATTGAGTTTTTTGATCAAAACAAAATTAGCTATGTTGTTGAGAATTTTTATAAAAAACCAATTAGTGATAAGCGTTTTAAAGATATTTTAAGTCTTAGTGAGGATGGTACTGAAAGTTTGTTTTCCAAACGTGCTGATCAGATTAAAGCAACTAACAGTGTTAGTGTTGAAGAGTTGAGTATCAGTGAATTAATTAAGCTAGTTAGAGAACGTCCTTCTTTACTAAGAAGGCCTATTATCATCCAATATAATTCTTCAGGAATTCCTAAAAGGATGCGAATTGGTTATAACTCATCTGAAATTAAGGTCTTTGAGCGTAAACTAATAGAACCAAAGCCTATAATACAACAATAACATCTAAATGAAATACAAAATTTTTGCTTCCACAACGCCCCAAACTGAACCTGTTCTCAATAAGTTAAGAGCAGTTTTAAAAACTTGGCAAGCGGTTGAGAATGGTTATGAATATGTGTTTGTTTTAGGTGGGGATGGTTTTTTTGTTTCCACACTTGCTAACTATAACTGTGATAGTTGTAAGGTAGTTGGTATCAATACTGGCCACATTGGTTTTTACACTTCTTTTAATGGAGATGATCTTGATGAAAATTTCATTTCAAAACTAACTAGCTTTGAGTTTAAAAAGATTAATTTACTGGAAGTGAAAACTAAAAACCACAGTTTCTTAGTTTTAAATGAACTTGCAGTTTACACCAACACTGCATATCCAATTAACATCTTTATTGATGATAACCACTGGGAATCATACCGCGGTTCAGGGTTGCTAATTGGCCCAAGAACAGGTTCAACTGCCCTAGCAAAATCTGCTAAGGGAGCGGTTATCTTTCCAAATGTTGATGTTGTTCAAATTATTGAACTAAACCCCTTATTACATCCCAACCAAATCACAATTCAATCTCCGATTATTCTGCCAATGCAAACCAAGGTTGAATTTAGGATCAAAAAAGCTTTTAAAGCAGAGCAATTTCCCAATTTTTATGCAGATGGAATCAAGCTTGATTTAAAAAATGAAGATACCAGTATTAGCTTTCAGTTGGTTTTATCAAGATCAATGTTTCACGCTTCTTTAAAAACTAAGGATTTTATTGATAAACTAAAATCAACTTTTATTAAGCAAAGCTAATGAAATGGTTGTTGTGATTGGGTTATATTTTTAGTTTTGGTTTACTCTATCTTTGGATAGTAAAAAAATCAAAACAAATTGCTCAACAACCTAACACAAAACTGGTTGAATCAACTTCTATTCCTTTTAAAGTTAAGGACTTTGTTAGTGCTTGTGGTGGTAAGGAAAATTTTGTTAATATAAAAACAACACCAACTCAATTAATAGTTACTTTTAAAGATGTTAACTCAGTGAGCTTAACAAAGCTTAATGCGCTTAATATCAAAGGAATTAACAAAAACCAAAACCAATTTCGTTTTGTACTTGGTAACTTTGTAAATGAATTGAAAAAAAAGATAGAAGATGAACAATAACATTACTAACAGTATTGCCCAATTGTTTTTCAACACTTCTTTCTTTGCTTTTCTGTTTTTAATCATTATTGCGTTTAATTTATGCTTGTTTGCCTATCTTTATTTTCAGTACCGAATTTATAAGAAAAACCCTAAAAAAGCTAACAACTTTAAAGCGAATGAATATGAAAAAATTAAGCTATTAAAAAACCAAAATTTCACTGAAAGTAATAAATTAATTGCAACAACTAATGAGTTAAATGAACTTACTAGTCAGCTAGATAATATCTTGGTTAGGATTATCAACAAACCACTAGCAAAGTTAGTTAATGATTTTTTAGATGAACAGATTAAACAGATAGTTAAGCTAGATAAAAACAGTTCTGATTTTCACTCAGAAAGTGATAACCTCCCTTTTTATACCAAACTCTTTAATGATTTTCACTTTGGTGTTGATAAATTAATAAACATTAACATAAAAAACCCTCTTTATAACTGGGTTTATAGCCCCAGTTTTTTAATTAGTGAAAGTGATTTTCGCAAGCTTAATGGTATTAGTGGTATCAATAAAAAGCTTTTGGTTGAAAAACTTAGAATTGAAGACATTGTGTTTACAGATCTAAACAAAAAGTATGAAGTTAATGTCTTGACAGAAAGTCCTATTAAAGCACAAAAAACAGTGTTAACTGTGCGCAATATCCTGATGAACGATTATGTTGATAATGAAAGGATTGAATCATATGTCCAACAAGCTAACTTCTTTTTTACTGAGCACTGTAAAAAGATCGGTAAAGAGATCTTAGAATCACTTAATATTTTTATCTCAAGTAGTTCACTACACCGTCATTTTGGCTTTTTAGCATTTCGCTATTCATTTGGACAAAATGTCTTATCCCATAGCCTTGAAACTGCATTTTTAACTGCCCACTTAGCAGCTTTAATAGAACTTGATAGTGAACTGAGTTTAAAGTGTGGATTGCTCCATGATATTGGTAAATCTAATGATGATAATGGTAAAGAGAGCCATACGATTACAGGCGCTAAACTCGCTGAGCAATTTCAACTACCTGATGACATTAAATACACAATTGCTAACCACCACAATAAACATATTGACAATACCTATTGTCGTTTAACACAAATTGCTGATAAACTATCTGCTGCTAGAATTGGTGCTAGAAGTGATAGTTCGCTTCTTTTTAAACAACTAAAAGATGAGTTGAAAAAGATTGTTGATAAAACTATTAATAATTTTCATACAACGATCTTACTAGGTCAAAGTGGTAGAAGGTTAATGATTTGACTTGAAACTAAAAACCAAAATCAACTGTTAAGTAATGAGCAAATTATTGAAATGGTTGAAAAGATTAAAGCTGAAATTGCTAAGAATCCAATTACAAATCACTTCCCTATTAAAGTTGTAATTAGATATAACTTTGAACACAGTTTTAACACCAAAAGCTAAGGATGCAATACAGTGCTTTAATACCACTTTTTATATTACTAATTAGCTTAGTATTATTTTGTTTCAGCTTTAGAAAAAATCAAAGTGAAAATCAGATAGTGAAAATCTTATTTTTTGCTTATTGCATCGATTTTTTAGCTTTAATTCTTGCTGTAATGTTGCTCACTTTTTTAAGTCATGGGTTGTTAAGTTTAGCGATTTTAATCCCTGTCTTAGTTTTTCAATAATAATGTTTTTTGTTATGGTTATTAGTCACTATCCCCTTATGAAAAGACTATTTGGAAATTAAAATTCTAAGTACCATGGAGTTGAAAACCCCTAACTTTAAGCTAATTGATGAAAAGATTGCTGAATTTAATAAGAGTAATGAAAACCTGATTGTAAAACTACTTCAAAAAGAAAAGGAATTTGCCACAAACCAAGTTACTGTTCAGTTTGATACTCAGTCAAAAAAGTCAGAAGAAGTGAAAAAACCTAGTAAAAAAATACTGAAAAGTTATCACTTTCTAACAGTAGTTATGGCAGTTAATTTGCTAATAACTGTTGGTTTGATAGTTGGCTTAATTTTTGCTTTCAGATATTAGTTGGTAGTTTTCTTCTAAGGAACGTTTGTTGGTTTTATTGACATTATAGCCAAACCCTTTACCAGTTTCATATTTGGGTACTATGTGTAAGTGAAAATGAAAAACCACTTGACCTGCAATTGCCCCTTCATTAGAAACATAGTTTAAACCAGATGGTTTTAGTGTCATCTTTAGTTTTAAAGCGATTTGTTTTGCTAATAAACTAACTGCTTGTAACTCTTTTTGATCAGTTGAAGAAAAATCAACTGCATGTTTTTTAGGGATTACTAAAGTATGACCATCAGCTACAGGAAAAGCATCTAAAAAAGCAATGGCATGCTCATTTTCTCCAATTTTATAGGATGTAATTGAACCTTGAACAATATCACAAAAGATACAACTACTTGTGGTGTTTTTTTCCATAGTAATGGTTATTTATAACAAAAAGCATTCATAAGATTAAAAACATTGATGCAAAAATAGGGATTGATCAAATCCAAACATAACTGTTATATTTAGTTTGCGTAAAAAATCCATACACTGAATAGGAGTTTTCACCATTATCCCAAACATTTAAAAATGGAATAGTTTGGAGATAAAAAGCATAGATACTGGGATAAATGCCTGCTTTTAAAAGAAAGGATTGCAAATTCCTTGGCGGTTTGATAAATTTATAAGTTTTAAAAAGTAATCCAACATAAATAAAGTAAAACAACGGATGGATAACATGGAGTAATAGCTCTGAAAAAGCATTCCAGTCATTAATAAAAGAACCATTAAAACTATATGGTAATTGCGTTGTTATAGTTGAAAACCAACCCTGTACATCAGGTTCTATATTAACAATACCTGTGAAGCGTGATAAAGCATAAAAGTTAAAGAAAATAACTGTAAAAACTAGATAACCTGTAACACTTAAAACCAGTGAACTGTTTTCAAATCATCTGCTTTTATGGTTAAGAAAGTAGTATCATACTAAAAAGATTGCAAGGCTATTACTTTGGTAAGTAAAAGTATCAAAATTAGTAAACCAAATCAGGCTCATTTGACCACTGTTAGCAACACTAATCAGTGCAGTAATCACCATAAAGATTTGGGTGAATAGTGAAAAAGGTGCTAGAAAAATTAAATAATAATCGCATTTATTGTTAAGATAAAAGCAACGGTAAAAAATTCCAATTGATTTTTTAAGCACGATTTTTATTTAATGATATATGAGTTTTAAAAAGATTGCTGAAATGATGCGTCAAGCAGAACGAGAAACTAAGAAAAAAACATTAGCGTTTGAACAACAAGCCTTTGAATACAACTATAAAAATGGTGCGATTAAGATCACTATTTTAGGTGATCTTACACTTAAATCAATTAACATCGATCCTGTTTTGATTGATGCAAGTGACAAAGTTATTCTAGAGGAGATGATTATAGAAGCTACTAATGAAGCGGTTAGTGATGTGAAAACCAAGTATGATAACTTAGTTGAGAAAACTATGCCAAAAGTTCCAGGTCTTTTCTAATTAGATTTTCTTTTTTAATAAAGATCTTATCCTTTTTCATTTCCATAAATGTAGGGATATTAGCAAAGAGTGTCATTAGTAAAATTGCAACATAACTTAAACTAAAGCCCACAATAAACACAAGTTGTGGATCACTATTGCTTAAGTTAAGAAAGGTCGCCACATTTTTATATTCACCATTAGTTCAATCACTTGCTAAGATACCAGTTGTATTACCTGTGATCTGGCCCTTAAACACAGATACCATCAAAGCTACATAACTAAAGTAAATTAGAGCAAACACATGGATATAGAAAAATGAGATTAAAGAAAAACAGTTATCCCACAAGATAATTGCTAAGCTTACAAGTAAACTGAGAAAATGCATCATGAAATAAATCTGGTTATTTCCTGTACCAACAAAGATGAAATTCACAATATCTTGTTCATTAACAGGTGTAAAAATTAGTTCACCAAAAAGAGTAACCAAAGCTCCAAATAACCCAAAAACAGCTAACACCCCACTAATCTTTTTTTGCTTTAAAAAGACAAACACTGGCGCAATAACAGCAAAAAATGGACAAAGATCTAATAAAAATATCCTACTTAGTCGTAGTGAATCTAAATTTTGTGAAGATTGGGAATTTTGTCCATAAACATCAGGGAATAAATAAATAATCTGCCTACCAAAAAAAACATAGACAAACAGTACAATGGCTAGTACTTGAATTAGATAGAACCTAATCTTTAGTTTTCGAACTAAAGGAGTAAAAGCTAAAACAGAAATTACACCAGCAATAATAAGGATGATGACAAGGTAGTTAAGTGTCTGCATTACTTATTGCTTAAAATTAAGATAAATAATTTTCCAATTTTGTTTTCAATGAGTGATCGTTTAAATGATCAAGCCCAACATCGCTTGCAGAAACTTTTAAGGTTAAAACAAACTAATAATGACCCTTATTTAGTAACAAAAACTAGTCTAACCCATTCTTCAAAAAGCTTTCAAGTTGAATTTGAAAAATGTTCAGAAGAAGAGTTGAAGAAAAAAGCAACTGTCTCACTAGCTGGAAGGATCATTGCTATTAGACAAACCTTTTTAATTATTCAAGATTTTGATGGTCAAGTCCAACTTTACATCAATAAAAAAATCCATCCTAAGTTATTTGATTACTTTAATGAATTTGTTGATATTGGTGATCAAATTGTTGTTAGTGGTAAGCCAATGTTAACTAAAACAAAGGTATTAACTTTAGCTGTTGAAGAGATGAAAATCATTGCTAAGTGTTTATTGGTTCCACCTGAAAAGTGACATGGACTTACTGATATTGAAACCCGCGCTCGCAAGCGCTTTCTTGATCTTACCTATAACTTAGCAATGCGTGATGTTTTTCTGAAACGCACTAAGATTATTAAATCAATCCGTAGCTTTCTTGATCAAAATGGTTTTATTGAAGTTGAAACCCCCACTTTACAAGCTGTTTTAGGAGGAGCTAATGCTAAACCCTTTAAAACCCATTACAATGCTTTAAAAGCGGATTTTTATCTCAGAATTGCTAATGAAATAGCATTAAAAAAACTCATTATTGGTGGATTTAACAAGGTTTATGAAATGGGTAAAATGTTCCGTAATGAAGGGGTTGATACTACCCACAATCCTGAGTTTACCAGTATTGAAATATATCAAGCTTATGCAGATTTTGAAGTCATGCTTGTGCTTGTTGAAAAGCTGATTCAATCACTTTGTGAAAGCTTAAACCAATTTAGCTTTAACTGAAATAACAAAACGATTAATCTAAAAACACCATTTCATAAGATAACAATGGTTGAACTTATTAAGAAAGTTACAGGGATCGATTTTAATTCAGTAAAAGATGATCAATCTGCCATTTTATTAGCAGAAAAACATCATGTTAAACTAGCAAAACACCAACAAAATAAGCAACACATCATTAATTTGTTTTTTGAACAGTTTTGTGAACAAACATTAATTGAACCTACCTTTGTAACCCATTATCCAAAAGCAGTTTCTCCTTTAGCAAAACAAGATCCTTCAAATCCTGAATTCACCCAACGATTTGAACTTTTTATTAATGGTAAAGAGATTGCTAATGCTTACAGTGAGCTAAACGATCCTTTAGAACAAAGAAAAAGGTTTGAACAACAACTTGAAGAAAAACAGCTTGGTAATGATGAGACAAGTGAACTTGATGAATCGTTTTTAGAAGCATTAAGTTTTGGGATGGTAAACACTGCTGGGCTTGGGATAGGTATTGATCGTTTGGTAATGTTGTTATGTGAATGTAATTCTATCCGTGATGTTGTTTTCTTCCCCCAGTTGCGTGAACATAAATAGTTTTGATATTTTAATTGTTGGTGCTGGTATTAGTGGAATAGTACTAGCTAACATCTTAGCTAATCACAATAAAAGGGTTTTAATTGTTGAAAAAAGAGATCATATTGGTGGTAACTGTTATGATAAAGTTGATAGTAAAACTCAACTCTTGTTTCACCAGTATGGACCCCATATTTTCCATACTAACAACCAAACTGTTATTAACTTTATCTCACCCTTCTTTGAACTAAATAACTACCACCATCGGGTTGGTTTAAAATTGAAAAATAACCTAGATTTAACCTTACCCTTTGATTTTCAACAGATCTATAAACTAATGGGAAAAGATGGTAGAAAACTCGTTAGTTTTTTTAAAGAAAATTTCAGTTTAAATACTCATCTATCATTAGCAGAATTACAACTAATTGATAATCCTTTAGCACAAAAACTCTATCAGTTTTTAATTAGTAATGTTTATAAACCATACAGTGTCAAAATGTGGGGTTTACCATTTGCAATGATTAATGAGAATGTTATTAACAGGGTCAAGATAGTTTTAAGTGAACAAAGCAGTTATTTTCCTGATGCAATTATCCAGGGATTACCTAAATCAGGTTATACAAACAGTTTTCTTAAGATGTTAGCCAATCCCTTAATTGATGTGCAGTTAAACTGCAAAGATAACCTTTTAGTTTATCAAGATGAAAAACTGTTTTTTAACAATAACTTAATAGAAAAACCAGTTGTTTACTGTGGCTTAATTGACAAGCTATTTAACTTTTGCTTTGGTCATTTGCAATACCGTTCTCTTGCCTTTAGTTGAAAAAGATTTAACCAAAAAAAATACCAAACCTACCCTGTTGTTAATATGCCTTTAGCTAAATCAATCACAAGGAGTGTGGAATACAAACAACTAACAAACCAAGGTTCTTTCAAACCGCAAACCATCGTTAGTTTTGAAACCCCTGGCAGCTATGCAATTAACGATCCTAGGTTTAATGAACCTTATTACCCAATTAACAATACACTAAATGATACTCTTTTTAAAAAGTACTGAAAAAAAGCAAGTAAGTTAAAGAATCTACACCTTTTGGGAAGATTAGCAACCTACCAATACATTGATATGGATAAAGCAATCCTACTTAGTATTAAAAAAGCCCAACAACTGTTAAGTTAATGGAACAAAAAAACATTAGAAATTTTTCTATTATTGCCCATATTGATCATGGTAAATCTACCTTATCAGACCGCTTGTTAGAACATAGTTTAGGCTTTGAAAAAAGACTATTACAAGCGCAAATGCTTGATACTATGGAGATTGAAAGAGAAAGGGGTATTACCATTAAATTAAATGCTGTTGAATTGAAAATTAATGTTGATAACAACAACTATCTTTTTCATTTAATTGACACCCCTGGGCATGTTGATTTTACTTATGAAGTGTCTCGTTCTTTAGCAGCTTGTGAGGGAGTTTTATTGTTAGTAGATGCAACCCAAGGAATTCAAGCACAAACGATTTCCAATGCTTATCTTGCGTTGGAAAATAACCTGGAAATTATCCCAGTTATTAACAAGATAGATATGGATAATGCTGATATTGAAACAACAAAAGATTCACTCCATAACTTATTAGGAGTTGAAAAGAACAGTATCTGTTTAGTATCTGCAAAAGCTAACTTAGGGATTGATCAGTTAATTCAAACAATTATAGCTAAGATCCCCCCACCAAAAGGAGAAATTAATAGACCTTTAAAAGCATTACTCTTTGATAGTTACTATGATCCTTACAAGGGGGTTGTTTGTTTTATTAGGGTATTTGATGGTTGTTTAAAGGTTAATGATAAGGTTCGTTTTATTAAAAGTAATTCTGTTTACCAAATTGTGGAACTAGGGGTTAAAACCCCATTTTTTGAAAAAAGAGATCAATTGCAAGCAGGAGATGTTGGTTGGTTTTCAGCAGGGATAAAAAAACTTCGTGATGTTGGGGTTGGTGATACTATTGTTAGTTTTGATGATCAATTTACAAAACCCCTAGCAGGTTATAAAAAGATCTTACCCATGATCTATTGTGGTTTATATCCAGTTGATAACAGTGATTATCAAAACCTCAAGTTAGCGATGGAAAAGATCATAATCAGTGATGCAGCATTGGAATATGAATATGAAACATCCCAAGCGTTAGGTTTTGGGGTTAGGTGTGGTTTTCTAGGTCTTTTACATATGGATGTTATTAAAGAAAGATTGGAAAGAGAATACAACCTAAAACTCATCTCAGCTCCCCCTTCAGTTGTATATAAGGTGTTGTTAACAAATGGTAAAGAGATTAGTATTGACAATCCCTCTTTGTTACCAGAACGCTCCAAGATTAAAGCAATCAGTGAACCATTTGTAAAAGTCTTTATTGATTTACCTGATCAATATTTGGGCAGTGTTATTGATTTATGCCAAAACTTCAGGGGTCAATATGAAAGTTTAAATGAGATTGATATCAACAGAAAAAGAATCTGTTATCTGATGCCTTTAGGGGAAATTATCTACAGTTTTTTTGATAAGTTAAAGTCGATTAGTAAGGGTTATGCATCGTTAAACTATGAGTTTTATAACTACCAACATAGTCAACTGGAAAAAGTTGAGATCATGTTAAACAAACAAAAGATTGATGCATTATCTTTTATCAGTCATAAAGACTTTGCTTTTAAGCGGGCAAAAAAGTTTTGCACTAAGCTCAAAGAATTGATTCCCAAGCATCTGTTTGAGATCCCTATCCAAGCAACAATAGGGAGTAAAGTAATAGCAAGAGAGACAATCAAAGCAGTTAGAAAGGATGTAATAGCTAAACTTTATGGAGGGGATGTTAGTAGAAAAAAGAAGTTATTAGAGAAGCAAAAAGAGGGTAAAAAACGCTTGAAAGCAGTTGGGAGTGTTCAATTACCCCAAGAGCTATTTAGTCATTTGCTGAAAGATGAAGATTAACATTTTTATAATTTGTTATTCTTTTGTATTGGTTGATACTTTAACAAAGTATTCACAATAAAATTTCAACACTAATATAAACAATGATAAAGGATTTTAATCCTGGTGATTTTATTGGTAAAAAACCAACTAAAATCTATGCTTTTGGTGGTATCCAAGAAGTTGGTAAAAACATGTATGGGATTGAATATGATGATGAAATCATCATTATTGACTGTGGCATTAAATTTGCTAGTGATGATCTACTTGGCATCAATGGGATTATCCCTAGTTTTGAACACTTAATTGAAAACCAAAGTAAGGTTAAAGCATTGTTTATTACCCATGGTCATGAAGACCATATTGGGGGTGTACCATACCTTTTAAAGCAGGTTGATATTCCTGTTATCTACGCACCAAGGATCGCAGCATCATTAATCTTGAAAAAGGTTAATGAGCACAAGGATGCTAAGCTCAATAAGATAGTTACTTTTGATGATTTTAGTGAGTTTCAAACCAAACACTTCAAAATTGATTTTTACCGGGTAAACCACTCGATTCCCGATGCTTTTGGAATCTGTGTGCAAACCCCTAATGGCAACATTGTTCAAAGCGGTGACTACCGGTTTGATTTTGCTGCTGGGAGTGAGATGTTAGATGTTCATAAAGTAGTGAAAATTGCCGAGCGCAATGTCCATGTTTTTATGAGTGAATCTACTAATGCTGAAGTACCAGGTTTTTCCCAAAGTGAAAAGTTAATTTACAGAAACATCCAAAAGATCTTAAAAGAAGCAAGGGGTAGGGTTATTTTAACTACTTTTGCATCTAACATCACACGGATTAATGAAATTATTGAGATTGCTTTAAACAACAAACGCAAGATCTGTTTATTGGGTAAATCAATGGATGTTAATGTTAATATTTCACGCAAAATTGGATTGATGGCAATTGATAGTAATGATATTGTGGAAGTTCGTGATATCAAAAACTATCCTGATCGTAATATCTTAATCTTGTGCACTGGTTCACAAGGTGAGGAGGCTGCTGCTTTAAACACAATGGCACGTGGTAAGCATAATTGGGTGAGCTTAAAATCAACTGACACCATTATTATGTCTTCAAATCCAATTCCAGGTAATTATGCTGCAGTTGAAAACTTGCTTAATGAACTCTCTAAGTTTGGTGTTGCTATTTATGAAAATTCATCCCAACTAAAACTACATGCCTCAGGTCATGCCACTCAACAAGAGTTACAGTTGATGCTAAATTTAATGTTTCCTAAATACTTAATTCCTATCCATGGTGAATTTAAGATGATGCGAACCATAAAAAACATTGCTAATGAATGTGGCATTAAAAGCGAGGATGTGGCGCTTTTAAGTAATGGCCAAGTAATGTATTTAATTGATGAAGAACTTTATTATTCCAATGAAATTATTAATGCTGATCCTATTTATATAGAGAGTCATAACTCTTCTCCTGATCTTGCAAGAATAATTAAGCAAAGACAAATCCTTAGTCGTGATGGGATGTTTGCTGTTATTGTTGTTTTTGATAAGAATAATAACATCATTGGGATTCCAACCTTAATAACAAGGGGTTGTTTCTTTGCACTTGATTCCAATCCTTTAATGACAAAGATAGCCCATTCTGTTAAAAGAACTTTAGAAAGTGTTATCCAAAGTAAGAAGTTTAATAGTCATGAACAACTAACAAAGGAATTGAAACGAGTTTGTAAGGAAACTGTTTCTTACTTTATCTGAAAAAATAAAAACCGTAATCCCTTAATTTCAACTGTGCTTTCCTGGATCTAATTCCCCCAACCTCTATTTTCTGTTACTAGTGCCAAAAGTGGTATTAGAGTACCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACCCAATCATCCTTCAACCCCACCCAAAGGTTGAAGAGTGGGAGTCCAATGAAGGATACAGGAAAGATGGGGGAGAAACTCAGTGAAACAACTGCTTCATCCATGAGTGGTATGGCTACATCCACTCGAGCCAAGGCCCTTAAGATAGAGGTGGAAAGGGGGAGTAATGTCAATCAAGGCGAACTACAATCCAACGACTTTGCCAAAAAGCCGTTTAAAGATGAGAGCAATAAGAAGTTGGATTCACAGAAGGAGTTTCCCCAAGGAAAGGTTTGAAAACCGGTGTTGAGTACAGATGAGATAACAAGAGAGAGGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAATGGTAAACCCTTCTCCAACTCCCCCTCCCCTTCAACTTCTGCTTCCTCAACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACATTTAAATAAAGAAAACACCCGGTGGGTGTTTATACCTAACTTTTCACCTGACATCTGAACAGGAGCAGGTTATAGAAAAGCTAATAACAACAATAACGGCATTCCTTTTGAACAGGTGAAACCTAGCAATAGTAGTACCCCCTTTAATCCCACCTCAGCTGGTGGCTCCTCAGCCAAAAAAACAACCACCTATTCCTTTTTACCCAATTCCATCAGTCCCACCAGTGACTGGATCAACGCATTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAACTGTTGTTAAGAGCACTGTTAGGAACTATTCCGGTCTTGATCAATAAGAGTGGGGATAGTAATGATCAATTTAACAAGGATAGTGAGCAGAAATGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTTATAAGATTTTTACTTATTTAATAGTTAAAAAAGTTTTGAATTTTTCTTAGTTTTTTATTTGTTTAATATTTAAGAAAGTCTCAAATTTTTATCAGTTTATTGGTCAAAGAAGTCGCAAATTTTTCTTAGTTTTTTATTTGCTTAATGGTTAAAAAAGCGTTAGTTTTTACCTTTATTTAATTAATAAAAACATTAAACTTCGCCACCCCCATCACCGATTCTAAAAGTGATCTGGTTAGTTTGGCACAACTTGATTCTTCCTATCAAATCTCCGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCCAAGGATGTGAAGCTTACATATAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAGTCAAGCTAACAAACCCGCCTACATCGTTGAATTTACTAATTCCACCAACATTGGCATCAAGTGAAGGGTAGTGAAAAAATATCAGTTAGATGTACCGAATGTTTCAACAACCATGAACGAAGTTTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCCAAAGAGAAGGGTAAGACACAAGTAGCGGTACATCTGGGTAGTGGGCAAGCAAATCAGTGAACCAGTCAACGCAACCAACATGACCTAAACAACAATCCCAGTCCCAATGCTTCAACTGGGTTTAAACTCACTACCGGCAATGCGTATAGAAAATTGGATCAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAACGAAGCTAAAAGTGATGCGCCCCTAAGTACAGGAGGGGGTGCTTCTTCTGGAACATTTAATAAATACCTCAACACCAAGCAAGCGTTAGAGAGCATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTACCCAACTCTATTATGCTTCTACCAGCAAGCTAGCAGTCACCAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGAGCGGAGTGCACAGGAAAATGCAAGTAACAAACCCACCTGGTTTGCTAATACCAATTTAGACTGAGGGGAAGACAAACAAAAACAATTTGTTGAGAACCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACTTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGCTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAAGGATCTAGTGACCAACGACACGGGATTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCTCATTCGTTGTTCCTTATTCGGGGAATCATAGTAATCAAATTTCATCAGGAACCATTAAAACTGCTTATCCTGTGAAAAAAGATCAAAAATCAACTGTCAAGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATTTAAACATTAAATAAAGAGAATTCACCCAAATTATTTACTTATTTATTAACTATTGTTACCCAATTTTTCTCTTTTTATTTGTTGTTTTTTTACTAATTAAATAAGCAGTCTTTCTTACAAAAAAGAAAAATTCATATATAATCTTTGCGCTGTTAACACCTTTGTTAACGCCAAAAATGTTCTTTCAAAACTGGATGCAATCTGTCAATTTTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGAAGTAGCAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAAACTAGCTAATACCGCATAAGAACTTTAGTTCGCATGAATTAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTAGGGTAATGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTTTGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTCTAGCAGGCAATGGCTGGAGTTTGACTGTACCACTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATCAGTCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGAGCGATCCCTTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTTTAACGAGACTGCTAATGTAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTAGCCAACTTGTAAAAGTGAGCAAATCTGAAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGCTGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCTTTATTGGAAGCGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTTCAAATGGAGTTTTTATTTTTTATTTATCTTAAACACCCATTAATTTTTTCGGTGTTAAAACCCAAATCAATGTTTGGTCTCACAACTAACACATTTGGTCAGTTTGTATCCAGTTCTGAAAGAATGTTTTTGAACAGTTCTTTCAAAACTGAAAACGACAATCTTTCTAGTTCCAAAAATAAATACCAAAGGATCAATACAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAAAAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGGAGGTGGTAAAAACCTGAGATCCGGAGGTGTCCGAATGGAGCAATCTGGTAGCTTGCAAAAGTTACCATTAATTAATGAATTCATAGTTAATTAAAGCGATACGTGGTGAAGTGAAACATCTCAGTAACCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACCTATCTGAGGATAGGGGTTGTAGGGCTTGCATTATGGAAGTTAAAAGGATAGAAGAAGCTGTTGGAAAGCAGCGCCAAAGAGGGTGATAGCCCCGTATTTGAAATCTTTTTAATACCTAGCAAGAAACCTGAGTAGCTCGAAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAGAGAGCGTTTGTTTGTTATCATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATTAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTAAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTTGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATATTTTTATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAGAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTATCCACCCCCATTATTGGATTTGGGGATAAACCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACACTGAGTGGTGATGTCGAGTGAACGAGTGATCAAGTAGCGAAGGTGGCAATTAATCATGCTTTCAAGAAAAGCTGCTAGGGCTAATTTAACTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATCTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCAATTTATTGCAAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAAAACACCACCATGTAGAGAATAGGTAGGAGCAATTGATGCAAGTTCGCAAGGATTTGTTGATGTGAAATGTGGAATACTACCCTTGGTTATGTTTTGTTCTAATTGGTAACTGTGATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCACAAAGGTACCTTCAGTACGGTTGGAAATCGTATTTAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTTCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCACAGGAAGATTGAAGAGCTTTGCTTCTAGTACGAGAGGACCGGAGCGAGGACACCGCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCTGTTAAAGGAAAGTAAGAGCCGTTATAGACCATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGTTGATAAAAGGTATTTATCAACTAAAAGATTGTCGTTTTTGTTTGGTGCTAATATCGCTGTGGAAACACCTGGAACCATCCCGAACCCAGCAGTTAAGCACAGTGGAGCTAAATGTAGGTAGTAATACTGAGAATAGGTAAGCACCAAGCAAAAATTAAGGACTATAGGTTTAAAAACCTATGGTCTTTTTTTATTTAATGAATAGTTTTTATAACTAATTTATCTAAAAAAATCGAACAACCTTTGACTAAGTATACGCTCAATGGCAGTTTGGCCAAAGAGAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAACTAATTGATCCACTCAACGCGCCACTCCATTGGTCTGAATGACAATCCCAGCCCAAATGCTTCAACTGGGTTTAAACTCACTACCGGCAACGCTTATAGAAAATTGGATCAATCCTGACCAATTTATCAACTAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAATGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGCCTCTTGTTTCAACAAGTGGAGGTGGTTCTTCTGGAACATTTAATAAATACCTCAACACCAAACAAGCGTTAGAAAGGATCGGCATCTTGTTTGAAAGTAATGGAGAGGCGAGGAATGTGGTTAGCCTCCTTCCAACTCTACCAACCCAACAAGGTGAAGGCTTACCAAACCACTAACACCTACAACAGGTTAATTGAACCTGACAAGTGACAATCAAGTAGTGATTTGAACAATATGACCAACTTGTTAAAACTCCTAACAACTAAAAACATCAAAGCGAAATTGGGGAAGGACACCCAATCAATGGGAAATAATAATGGAGGGGGTGTTAGTCAAACCTTTTCCTTTGTTGTTCCTTATTCAATGAATCATACAAATACGGGAACTAGTGGAACCATTAAAACTGCTTATCCAGTGAAAAAAGATGAAGCTTCCCAAGTAGCGATCAATTCCTTGATCAACGCTACGCCATTGAATAGTTAAAAACCTGATTTATTTTTATAGAAACAGTTACTTTGAAGATTCACTACTAATAATTTAAATTAAGCACTCATACTAATATATTAATCCCTTTAGATTGCTTAACTCTTTAAAACTTCCCTTTATAATCAAATATCGCCATGAACGACTGACAGTGACTGAAAAACAGACTTGTTAATTCCAAAACCAAATCTGTTAGTTTCTGATTACCACAAACCTCAAGTAATATCATTGATATTGCTGAATTAATTAAGTGCTGTAGTGAGTTAAAAAACACTTCTATTAATGGTTTAATTGACTTTTTGAACCAACAGGATAAACTTGAATTTAACTTAACAAGATTAAAGGAGATAGATGTTGAAGATGGTAAGCAACTATTTGGGATAGAAACTAGTGTTTATAAACACTTTCAAAATGAAATTGCTCGTTTTTATAAACAAGTAAACAAACACTTTCGTGAAACAGGTAGTGAAAGTTTGTTTTTAGCTTTACCAGTTATTGAAGGGATTAATGAGTTTAACGATATCTTTCGAGCTCCATTACTTTATGTTGGAGTTAAACTCAAAGTTTCCCCACGCTTTGAACGTTTCTGATTAGAAATTAACAAAGAAGAGATCTTTTTAAACCCTACTATTATTGGAGTTGAAACCAACAAACGTAATAGTTTGTTTAAAAATAACTATGATACTACTAAGATAGATGTTAATGATGCTTTAAAGGTATTTAGTGAACTTGAATATGAGTTTAGAATGCCTTTAACTTCTGAATTGAAGAGTTTTAGTAAAAAAGCAAAGAGTGATTTTAATACTGAAAAACGAACTAACTATCTTATTAACAACGTTCTTTTGGGGATCTTTGATGTTAAGGGTGATCAGCTGTTCCAAAACTTTAATGAGATTCTAAACACTGATCCTGATGTATTAGATGAACTTCTAAAAGATAGAAGAGATCTGTTGTTAGAAAACCGGGAATTCCGTGAACAATTTGATTTAAAAGATACCTATCTCTTCAGTCACCTTGATATCTACCAACAGTATGCAGTTAAGCAAGCTTTACTTGGTGATTTAATTATTGAAGGCCCACCTGGCACAGGGAAATCTGAAACAATTGTTAATATCTTAGTTAACCTTGTTTTAAACAACAAAAAAGTATTGTTTGTTTCTGAAAAAGTAACTGCACTTGATGTTGTTTACAACCGTCTTGGTAGTTTTAAACACATCGCACTTTTCAACGCTAGTGTTGCTGCTGAAAAGAAACGCTTTTATAACCAGTTTGCTGAGTTTGAAACTTATTTCACTACTTACTTTTCCAAGAAAGATTTGGATGCTACTTTACCAACATTTGAAGGTAAATGGGTGGATGATATTTTAGGGGCATTTCAAGCATTACAAGCTCTTTATGACACCAAGATAAATTCTGGTGAAAATCTGTTTAGTTTCAAAGAGATTGTCAGTAGCTTTCAGATGTTGGATGCTAGTTACATCAAGATTAAAGAATATGAACGTTTTGATGAGTGAGTGCGCGTCTTTTCAAATCCATTGTGATTAGAAAAACACTTAAGTTACCAAGAGTTGAAAAAAGAACTTAGTCAGCGCTGGAATGGTATTGATAATTTCTATCAGTTGCAATCGCTTCTAAACCAAACCAAAAAACGAAAGGTCTTAAACTATGTGTTGGAACACTTTGAACAGTTTAATACAGTTATCAGTCCTAAGCATGTTTTGTTCTACAAGCCTAGCAATAAATCACAATTGCTCTTAAAACAACTGAAACAGGATGTTGAACAATACACTAGTTTACAACGTTTCCAATCTCCTACTAAGTTTGAAACAATCAAGTTGAATTTCATCAACCAAGTTAATGAAAACCCAACCCCATGGTTCTTTTCTTGATTTATCCAATTTCATGCCAAGCCACTGTTGGAAAAACTCGTTAGTTTTGAGTCAAACATTATTAAAACAAAACAAGCTTATCTTAATGGGATTGAAAGCTATGTAGCAAGTTGTAAGAAACTGCTTAAAACAACTATTTTAAACAACTTTTTTCAGCTTTATCAAACTAATAAAGATGAACTATTGGAGATCTGCAGACAAGCAAAAAACCCAGTTTTAAAAGAGATTACTTGGTGGTTTAAAAAACATTTTGAACTCTTAAAAAAACTCTTTCCAGTGCACATTATGACCCTTGAGTCTGCAGCAACTCTAACCCCTAACCAACGTGGTTTGTATGACTATGTGGTTATTGATGAAGCTAGTCAAGTATTTCTAGAAAGAGCAATTCCTATCTTATTTAGAGCTGATAAGTACATTATTGCAGGGGACACTAAACAGTTAAAACCAGCTAACTTCTTCCAATCACGTGCTGAGTATGATGTTGATGAGGAGTTTGAAGATGGCAATATAGAAGCTGCTGTTCACTCCAGTTCTCTACTTCATTTTTTAAAGAACCGTTCAAGAATCTTAACTTTACTTAAGTTCCACTACCGCAGTGATTCAGCTGATCTGATTGCTTTTACTAATAACAGGATCTATGACAATGAATTAATCTTTATGAATAAAGCTAATGCTGATCAAAGGGTTTTTATTGTCCATGATGTAATAGATGGTATCTGAAAAAACAACCGGAATCTCCAAGAAGCACGTGATGTTGTGCAACGCTTAGAACAACTAACAACAACCAATGATTACAAAAAGTCACTTGGTGTAATTTGTTTCAATAAAAACCAAGCAGATCTAATTGAATATCTAATTGATAAACAAAACAATCCATTACTTAACGAGTGAAGAGAAAGACAAAATGATGTTGGTGAATATGAAGGATTATTTGTGAAAAACATTGAAAATGTGCAGGGTGATGAAAGGGATATCATCATCTTTTCTTTAGGTTATGATCGTTCAGTAAATAGTTATGGTCCTATTAGTAAGCAAGGTGGAGAAAACAGACTAAATGTAGCGATAACTAGAGCTAAACAAAGAATAGAACTGTTTAAGACAAATAGAGGAGAAGACTACAATGGTTTAAGCTCCAGTTCCTTGGGTAGTAAACTGTTAGTTGAATATCTGCTTTATTGTGAAGCGATGGCTAAAAACCAGGGTGAGAAAATCACTTTTCAAGCGGTAAAAAGAAAAGAAACAAAAGCAAAGTATGAACTGGCAGTTGAAAATGATTTCTTCAACCAACTGCAGGCAATTTTTGGTGGAGAGTTTGAGATTAAACGTAACGTTAATGAAGGGGCTTACTTTTTCTCATTTGTCTTTTACTTTAATAATATCCCTTATCTTGCCATTGACTTTAACATCCCCATTCCCACTTCAAGAAAACAAGTTATGGAAGGGATTTTATACCGTGAACAGTTTCTCAAAAAACGTCAATGGAACCTAATTAACATCTGGATTGATGAGTGAAAATTAAACCCAATTGGGGTGATTTCTAAAATCAGATCAAGTTTAGCAGTGCATCAAAACCAGCATGAAGAAATATAATTAGATGAAGATAACTTTCATTTCTGGACAAGAAGTGTCGTTAGGCACTTCTTTTTTATTGTTTTCAAAAAAAATAGTTATGAATGAATTAAACCAACCCTTACTTGCTATTATTAAAAATGTTGCTAAAACCAAAAACCTTTCTATAGAAGAGGTGGTTTTTTGTTTGAAAACAGCTTTAGAACAAGCCTATAAAAAACACCTTAACTTTGTTAATGTTGAAGTTAACATTAACTTTGATAAGGGGATTATTAATGTTGAACAACTCTTTAATGTTGTTAGTGATGAAAATGAAGATTATGATGACTTTCTTGAAATCCCTTTACAAGCAGCTAACAAAATAAACAGTTCATTGCAATTAGGTGATGTGTTGCGAAAACCAATCCCCTTAAAAAACATTAGTAGTGATCTTATCAATAAGATGATTGCTATCTTTAACCAAAAGATTAGTGAAACAAACTTTAAAGCAGTAATGAGTGAGTTTAGTAGTGAGGTTGGGGAAGTGATTGAAGCGAAAGTTGAAGATATTGATACTAACAAAGAAGGTGGTTTAAAGGGTTATATTATTAACCTTGAAACTACAAAGGGTTATATCTCCAAGCGGGAATTGTCAAAAGGGGAGCGCTTAGAGATAGGTAAAAAATACCTCTTTGTTATCAAAGAAATCCAACGGCAAGCATCGTTATGACCAATTACTTTATCAAGAAGTGATACCCGCTTACTACAGTTTTTGTTAACTTCAAATACTCCAGAAATTGAAAATGGTACGATTGTAATCAAAAAGATTGAACGTTCCCCAGGAGTGAAATCAAAGATAGCAGTTATCTCCAATGATCCTGCAGTTGACCCAGTTGCTGCTATCTTAGGACCTAAGGGTGAGAAGATTAGGGGGATTAGTGAGGAATTTAATGGTGAGATTATTGACATTGTCTTTTGGAATGAAGACAAGTTAAAGTTCTTAATTAATGCCATTTTACCTGCAGAAGTCATTGGTTATAACATCTTGCAGGATGATGAGCGTGATACTAGTATTGAAGTTGTTGTACCTGCAAACCAAATTGCTAATGTTTTTGGTTTTAAAGGTGTAAACATTAGGTTAATTAGTAATTTAACAGGTTGAAATAGTGTTGATGTTTACAGTGAAAAAGATGCAAGTGAAGCCAACATTAAATTCACGAGGTTAAGCTTTGAACCTGAAGGGTTGTTTGGCATCAAAAAAAGAAGGGAAAAGATCATTAGTAATGATGCTACTGATAAAGTCTTTTACACCTCTAAAGACAATGTGATAGATGATGAGATTATTGTTGATTTAGCTAAAGATCTAATGGTTGATAATAAACAAAAACAACCTGAGCAAGTTGCAAAGCAAGTTGTTGAAAAATCACAATTAGAAAAACAAGTTACTCCAAAAGAAAAAGAGAAAGTTCAACCAAAAGCTAAGGTTCATTCTAATAGCCATTCCAAAAAACCAGCTAAACCTAATCAGATTTTTTCTATCACTGTTGATGCTAGTGATAAGAATCTTAAAAAAGATCAAGTTGATAATAACCAAACAAACCCCCAAACAAAACAAACATTTGATAGCTTTGATGATCTTTAATGCAACTAATAACAAGACTTTGTTTATTAACAAGAAAACATTTTGTTAAAAGAGAACTTTTACGTCTTGTAAAATTAGACAACCAACTTGAAATTGATCTTAATCAAAATCTCAAGGGCAGGGGTTATTATTTGAGTGTTTTTGGTTTAAAGCTAGATAAAAAACACCTCAAAGCTGTAGTTGAAAAACACCTTAAGGTTAGTTGTAATGATGCAAAGCTTACTGCAATGATTACCGCCTTACAACAATTAGCACAAGATGAAAAAAAATAGAGCTTTCAATCAGGTTAAAAAAACAAAGTTTGACGGTAGGATTAAAACCAGTGCCAAACACCAGTTACGTAATGTTAAAACCGGGGTTAAAGATGGTGTTTTTATCTATAAAGGTCCTTTAACTGTTAGTGAGTTTGCAAGTAAAACTAATATCGCTGTTGCTAACATTATCAAACACTTTTTTTTAAATGGTTTGGCACTAACAGTTAATTCAGTTTTAACAAATGAACAGTTAGCAGATGCATGTGTTAACTTTGGGTTTGACTTTAAGATGGAAACTGAAGTTACCCATGAAAATATTGTAGCTAACATCCAGTTTGAAGATAGTGATGATTTATTGCAACCAAGACCACCTATTGTTACTATCATGGGTCATGTTGACCATGGTAAAACTTCGCTTTTAGACACAATTAGAAAAACTAATGTAACTGCTAAGGAGTTTGGCGGAATTACCCAAAAAATTGGTGCTTATCAGGTGAAAAATCACCAAAATAAAACGATTACTTTTATTGATACTCCTGGGCATGAAGCATTTACTTTAATGCGTGCAAGGGGTGCAAAAGTAACTGATATTGTGGTGTTGGTTGTGGCAGCGGATGATGGGATTAAAAAGCAAACAGAGGAAGCAATTAGCCATGCTAAGAGTGCTAACACTCCTATCATTGTTTTTATTAACAAGATGGATAAACCAACTGCTAACCCTGATCTGGTGATCCAACAACTCAATAAGTTTGATTTAGTTCCTGAGGCTTGGGGTGGGAAAACTATCTTTGTAATGGGTAGTGCTTTAACTGGTCAAGGGATTAATGAGTTGCTTGATAATATCTTGTTGCTAGGGGAAGTGGAGGGTTATCAAGCTAACTATAATGCCCATTCATCTGGTTATGCAATTGAAGTACAAACTTCAAAGGGACTTGGCCCTATTGCCAATGTCATTGTAAAAAGGGGTACTTTAAAGTTAGGTGACATTGTGGTGTTAGGGCCTGCATATGGAAGAGTTAGAACGATGCATGATGAAAATGGTAATAGCTTAAAACAAGCAACCCCTTCAAAACCTGTGCAGATCTCAGGGTTTGACATTATGCCTGTTGCTGGGGAAAAGTTCATTGTTTTTGATGATGAGAAGGATGCAAAGTTAATTGCTAACAAGTTTAAAGAACAACAAAAACAAAAAGCTAACAACTTAACAGTTAATCAAACCTTAAAAGAACAGATTAAAAACAAGGAAATTAAGATATTAAATTTGATCTTTAAAGCAGATAGTGATGGTTCATTGCAAGCTATTAAACAAGCAGTTGAAAACATTAATGTTGCTAAGATCTCACTTAGTATCATCCATGCTGCAGTGGGGCAGATATCAGAGAGTGATATTATGCTAGCAAAAGCATCAGGGGCTTTATTGTTTAGTTTAAACTTAGGTTTGAGTCAAACTGTAAAAAACATTGCTAGTTTACAAGGGGTAAAATTAGAAGTTCACTACCATATCCCTAAACTAGCAGAGGAGATTGAAAACATCTTAAAAGGTCAATTAGATCCTGTTTATGAAGAGATTGAAATAGGTAAAGCGGAAGTTTTACAACTCTGGTTCCACTCTAAAATCGGTAATATTGCAGGAACCATTGTTAAATCAGGAAAGATAAAAAGAGGGAATTTATGTAAGTTATTCAGAGATAAAGAGATTATCTTTGAAGGCAGAATTGACTCTTTAAAAAATGAAAAAACGCCTGTTAATTTAATAGAAACAGGGAAGAATTGTGGGATAGTTATTAATGGTTGCAATGATATTAAGATTGGTGATATCATTGTTGCTTATGAAAAGCAGATAGTTAAAGATGGCAAGCTATAGAAAACAACGGATTGAAAATGATATCATCCGCTTAATTAATCGCACGATTATTAATGAGATCTATGATCCTGTTGTTAAGTTAGGTCATGTTAGCCATGTGAAGTTATCAGCTGATTTTTTTCATGCAGTAGTTTATCTTGATTGTTATGATCGTAGTCAGATTCAAACTGTAGTTAATGCTTTTAAAAAGGCTCAGGGCGTTTTTAGTCAAATGTTAGCACAAAATTTGTACCTAGCTAAAAGTGTAAAACTCCACTTTGTGAAGGATGATGCAATTGACAATGCTTTGAAAATAGAACAGATAATTAACTCTTTGAAAAACTAACAGCTTGAAGTAAAATTAATCCTAATGGACCCACAAAACAAGTCCCCAAAACCACAAGTTAAATCAACTAGATTGGTTGTCAAAAAACAACCAGCAGGTGTTGTTTTTCCCAAATTGAGTATTCCTGTTAATGATTTTGAAAAAACAGTTACTTTAACAAGAGCACAAAAAAAGGAAGCTAAACTTCTCAAAAAAGCCCAAAGGAAAGCTAATAAGCTTAATAATAAACAAGATAGTACCTTTTTCAATTCTGCTTCAGGTGAGACTAATAACACCATTCTTCCTCCTGGTGTTAAAAATCAAGCTGATAATAAAACTAACCGTTTTAGTAAATTTATTAGTTTTTTCACTTCTTCAAAAAACAAACAACCAGATGAAATAACAGAAAGGTTAGTTGATGATCCTACTGTTAAAAACCGTTTTAGTGCTTTTAATAAGAAGCTTATTTGAGTTCTAAAGGATAAAAAACTAAGAGCAAGAGCGTGGAAGATTGTTGGTTATACCAATTTAGTTATTGTGGCATTTTTTGCTGGACTTTTAGCAGTGATGAATAAGTTCATCACCCTTTCCTCAGTTGAATATCCTGCTATTGCTTTACAACTCCCTATTAACAATGCATTATGAGGGATTTCTATCTTTGTTATTAGCATTGTTACTTTACCGTTTTGAACAATGTTTATCTTGTTTTTAATGGGAGTAAAGGATGTGAGAACTTCGCGTTCTATCCATTATTTTATCTGGATAGTGTTAATTATTAATGTAGTTTTATTACTAGTTAGTTGCTTGTTGATGATTGCTGCTTATGCCCATCTTGATGGTTATAACATCTGAAGAAACTTAGAATCACTTAACCCTAATAACTAATGAAATCACTCTTTATTGGTTATTTTGATGGATTACATCAAGGTCATCTATTTTTAAAGCAGAACAGTAAGTTTGAACCAATGGTGTTATTAATTGATAACCCACCTTTAAAACAAACCAACTGGCTTTATGATTTACAACAACGGGTTGCACAAATAAAAACTTACTTGAAAGCAACTGTAGAAGTATTTGATGTTGCCAAACATAACATGAATGCACTTAGTTTTTTTGAACAACAGATTAAAAGATTGAATTGTGATGAAATTATTGTTGGTACAGATTGGCATTTTGGTAATGATCATAAGGATGGGATCTGGTTAAAGAAACTGTTTAAAAATACTGTTATTGTTAATAAAACAAACCTATCAAGTAGTGTTATCCGTAACTATCTAACTAATAATGAACTTGAAAAAGCTAACCAACTTTTAGTGGAACCTTATTATAGAGTGGGCACAGTAGTACATGGTTTAAAAAAGGCAAGGTTGCTTGGTTTTCCAACTGCTAACATTGTTATGGATAACCACTTATTGACTTTAAATAAGGGGAGTTATATAGTAAGAGTTTTATTAAATAACCAAACTTTTTATGGGATTGGTTTTATTAGCCAAAAGGATCAGGATTTGGTGTGTGAAACCCATATCTTTAACTTTAATAATGAGATTTATGGTTCACTGGTCAAATTTACACTGTTAAAGTTCATTAGAACAATTAGTAAGTTTTCCAGTCAAGCAGCTTTGCAAAAAGCAATTCAAAGTGATGCTAACTTTGCTTTAAAGTGGTTGGAAAACCAAAATTTAGATAAAATTTAAAATCATCCAAATATGGACAGTGCCCCCAGTGGTTTAACTTTAACTGTTATTATCCTTAGCATCATTCTGCTTGCTTTTATCAGCACAGTTGTATCAGCTTATGAAACAGCAATCACTTCTTTAACCCCTTACAGGTGAAAGAACTATATCAAGACTAACAACAAGCAAGATAAACTATCAACTAAGATAATAAACCACTTTCAAAACCACTATTCAAGTTGTTTAATTACTATCCTAATTACTAACAACATAGTGGCCATTATGGTTTCTAACATCCTTTTTTTAGCACTAGAACAAACAATTAAAAATGAGCTTTTATCAAGTGTTTTAAATTTGGTAGTTAGTGGGGTTTTAATCGTCTCTTTTTGTGAAATTCTACCCAAAACTTTGGGCAGAATTAATGTGATTAGAACCCTGGTTCTATTTGCTTATTTGGTTTATTTTTTTTATCTGATCTTTTGACCAATTACTAAGCTAACCAGTTTAATTCTCAAAAAGTATGAAAACCCCTTACCTGTTTCAAGGAAAGATGTTTATTATTTTATTGATGAAATTGAACAAAACGGTTTATTTTCCAAAGAAGATAGTTTACTGATTAAAAAAACCTTAATCTTTGACCAAGTACTAGTTAAAAAGGTAATGATCAAGTGAAAAAAAGTGGCTTATTGTTATCTTAATGACAGTATTAACTTGATTGCCAAGCAGTTTTTACAAAGGCAGTTTTCCAGAATGCCAGTAGTAGATAAAACTACTAATAAGATAGTTGGTTTTATCCATTTAAAGGATTTTTTTACAGCTAAAGAAGCAAACCCTAAGTCACTTGATTTAAACCAGTTGCTTTATCCAGTTGTTTTAGTTCAAGATTCCACCCCCATCAAACAAGCACTAAGACAGATGCGTTTAAACAGAGCACATTTAGCAGTTGTTAATGATAAACATGAAAAAACAATAGGGATTGTTTCTATGGAGGATATTATTGAAGAGTTGGTGGGTGAAATCTATGATGAACATGATGATATCCAACCGATCCAAGTATTGGATGAAAATGTTTGACTTGTTTTACCTAATGTAAAAGCAGCCTACTTTTTTAATAAGTGAATTAAGCCAGATTTGGTAAAATCAAAAAATATTACTATCCAGCATTATCTCGCTTCACTAGATAATGATAGTTTTGCTTGCCAAAATAAGCTTGACACTCCCTTATTTAGTGTTGAAGTGATAGCTGATAGCGAAGATAAAACCAAAATTCTTTACGAAATTAGAAAGAAGAGTGATGTTATTGCTTAGAGCATTATTTTTAGAGTTGAAAACCAACAAAAATTGTAAAGCTTTACTTTTGTTATTAATCCCCTTACTAGTTGGTTTAACCTTGATAATCTATGGGATTGTCTTATTTTCCACTGAAGGGGTAATTGACCATGGTGATCATAACCACTTAAGAGCAAGGTTTCAACTCACTTTAGAGGAGATTATTGTTTTTGTTGTTGGTAGTATTATCTTGTTTTTTACTTTAGCTAGCTTTTGTGTGAGTTGCTTTATGTTAATGAGAAGTCCTAAGCAAAAACAGCTAGAGGTTGATCATGCTAATAAAACTAATTTAAAACCAAAAGCAATAGTTAATTGTGATCTTTTTCAGTTGGGTGATTACTGTGTGTTTACATTTAAAAAACTTAGCTTTAAACAACGGTTTAAGCAAGATTTTTTTGCTAGAAGTAAGTTTTCGTTTCGCAGTGAACTGTATCGTCTTTGTTTGGTGGGAGTGTTAATTGCTCTCAATTTAGCGTTAAGTTTGATTGAAATTCCTGGGATAGTTTTACCTTGGGGTAGTTCGATCCAATTCCGTTTTTTTAATACGGCTATCTTGTTTATTGCTGTTAGGTTGGTGGGATTATTATCTACTTCTTTAGTGGCTTTAATCACCCCCTGATTACACCTGTTAATCCATCCTATTCACACCCCCATTAGTAGTTTGTTTTACATGGTTAATGACTTTTTGGTGCTGTGGATCTTTTATTTCTTTTTTTTCCATCTCTTTAAAGCAGAGGTGATCCAAACTACTACTGTAGTTGACAACAAACCTTTTAGTCAGTTAGTTAATACTAAAAAGACGAAGTGAACAAAGTTTTTTTCCTTACTTGTAATTAGCTTTTTGTGTGGCTTTATTGAGGGGTTAGGTTTTTACTTTGGTTATTTTTTAATCCTTGGTAATGTTTCTAGTTTAGGATTGAAGATCTATTATGATGGGTTACAACAACGTGATCTGATTAACAGTAGTAATGTTTTGTTCTTTTTAATGACAACAACTGCTATCTTCAGTATTAAATACATCTTTGAGATGTTATTTTTCTTTAGTGTTGAAAAGAATGTAGTTAATATTGCTAACCACTTTGGTTTGTATTAATCTAAACTTTTTGCAACTAATTAATTAGTTAACTAATAATGAAGTATGTAAAAGTACAGATCATCAATAAATCAACTATTGAACTGTTAGAGGATGCTAAAAAAGGGGAAAAAATTAATTTAGATTTAATTAACCAAGTTGATCAGACCAACATCCTCAATACGATCACTACTAACCAAAAATTAGCATGAGAAAAGGAGTTAAGTGCTCAATTTATCAATCAGCAGAATGAGTTAATTAAAAACTTTGAAATTGAGATCATTAAGTTAAAAACTATGCTTAATGACAAAGAGCAAGCATTGTTGTTAAAAACCAAATTAGAATTACAAAACCAGTTTCAAAAGCAGATAGAGAACTATATCAATGAGATTAACAAGCTCAAGTTAACCAATAAAGAGCTTGAAATTACTAACCAAAAACAACTAGAAAGCTCACTTAAACTACAGCGAAACGAATTTGAAGAGAAAATCAACCAGCAAAACTTAACGATTGAAAAACTGAAAATTCAACAAGCAAGAAGTAGTATCTGAGCAGTTGCTAAAAAAGGGAATGAACTTGAAAAGTGGTGTGAAAACCAGTATGAGTCTTATGCAGATAGCTTTGAAAACTGTCAGTTTACTAGGTATAAAACTGAAATTAACTTATTAGATGAAAATGATTTTCCTAATGAAAAAGCAGATTACATCTTTAGTTTCTTTGGTGAAAAAACCAATAAAATTCCGTTTTTATCAATTTGCTGTGAGATGAAAAGTGAGTTTAATGATAGTAAGCATAAATCAAAAAACAAAGATCATATTAGCAAACTGGTCAGGGATGCTAAACGTGCTAACTGCAAGTATGCTTTTTTAATTAGTGAACTTGAACTGGAGACTGAAAATGACATCCAAGTGCGCTTAATGCCAACATTGGAAAGTGGTGTTGAAGTCTATCTAGTTAGACCAATGTTTTTTATCTTAATGCTTAAACTTTTCTATAAGTTAGCTAAGAAGTTGTTTGCCCTTAACCGTTTTCAATCAGTTGAACTAATTGATAAAAATAAGTTAAATGAACAGTTTAAACAGTTGAAAGATAATTTTTTAACCAAAACCTTTTTAGAGATTGAAAAAGTGTGTAAAAGTAACTTAGTTGATATTGAAACACTTGAAAAAGCAGTGGTGAAACTAAGGGTTAGAAACGAACGCGTTTTAGATCAGTTACTTAATAAATGAACTAAGAAAATTGATAGCTTTGATTTACAGTTAACTAAGAAAATTACTAATAACTACTAGGGTTTAATTTGTTAGCTTATTTAGAAAAATTCAAAATAAGCAAATTATAATTAGGTGTCTTTCTTTACTAAAAATATGAAATTATACCGATCTTTAAAAGCAGCCCTGTTACCAGGGATATGCACTAGCATTTTACTTGCTAGTTGTGCTTCAACAAATACTTATCAAGACCAAAGGAATGCCTTGATTAGTTTGGCTTCTAATCGTGATACTTTAATTGCGAATGCTAAAAAATCCAAAGAAGAAGTGCAAAAAGAAGTTACCAAAATGAATAGTAGTACTTCATCAATGATGACAGCTACCCAAAGTGTTGCAATTACAACACATCAAACTACTGAAAAAACAAATAATTCTAAGTATGATCTAGACAAGCTTTTTAAGGATTACATCCTTTATGTTGTTGATAATTTTTCAGGACTTGTTTTTAAAAGAACTGGCGGTCATAGGATCCAGTTAATCGATAAGGATAAAGAGATTTTGGATGGTGGTAATCTAACTAAACATACCCACCACGATCATAACCATATGCATAATCATGAACATGAACATGAAGAACACCATGATGAGGAAGAGACAGAAGTAGTTGGCAGAGCACTATCTTTTACTAATGGCATCTTTCTAGTGATTGACTATAAAAAAGACTCTGAAAGAAAAAATATGAGTGGTTCAACTACTATGATGCACCAACACCACCATGAAGCTGAAGAACATAAAGAGGAACGTAAGCTCTCTTTAAACTTAAAAGCATACAAATTTAATACCCCTTTTAACATTAGTGAGTTTATTAGTGCTTGACATCATAAAGAATCTCATAATAGTGACACAGAGTTCAATAACCTTCACAATAAGTATGACAAGGAAGAATTGGATATTATTGACTATAACTTTGAAGAAAAAGCTGTTGATGAAACAATTGCTTAACTAAAAAAGATAGTTATTAACTTAATTTAGAATTAACTAATGACAGTGTACACTTACACTGTTTTTTTATTTAATTGAGAAAATATTGGGTTGATGGAACAACAAAACCCTGATCGTTTAAAAAAAGATAGGGAACTTATTTATGCAATTGTTACAGCTAAAGGTATCATTAGCCGTTTCTTTTGATCAATCCTTAGTTTTTTAATTACTAACCTTATCTTCTTTTTTGCAGCTTTTGTAGCGCTCTTAATTTATCTGTTAGCAAGTGTTGATAATCAGTTTGCATTTGTTTTTATTGCTGCAATTATCTTCATTATTTTTTACAACATCTTCTTTTTAAGTTACCTGTTGTTTATCTATTTTAAGGGCCAGAAAGCAATTGAAAACAACTGTAAGTACCTGTTAACAATCCTTGATATTAAGAGTGATGAACTGTTACCTTTTTCGCTTTTAGGTAGTTTAAGAAAAGGTTATATGCTAGATGAAATGCTCTTAGAACAGTAAATATTTGCTACAATCATAACGCTTTAGTTTTTAGTTGATACACCAAAATCCGTAGTCAATTTATTAACTAACTAGTGAACTAGATTTTGATGAATAGCGCTGTAAAATATCCTGAGCTGAAGATCAAACTTGAGTCTTATGATAGCACCCTTTTAGATCTCACTATTAAAAAGATAGTTGAGGTTGTAAAGGGTGTGAACATTAAGATTAAAGGTCCTTTACCTTTGCCTACTAAAAAGGAAGTGATCACCATTATCCGCTCTCCCCATGTTGATAAAGCATCCAGAGAGCAGTTTGAAAAAAATACCCACAAGCGCTTAATGATTCTTGTTGATGTTAATCAAGGAGGGATTGATAGTTTAAAAAAGATTAAGATCCCAGTTGGGGTTACACTGCGTTTTTCAAAATAGGTTATGGATGTAAGGGGAATATTTGGTGTTAAAGTAGGGATGAGTCAGATCTTTACTGAGCAAAATGAGTGCTTACCTATCACCATTGTTTATTGTGAAGCTAATCAGGTGGCTGGGATTAAAACGATTGCTAAAGATAATTACAACGCCACTCTATTAAGCTTTCAAACTGTTGATGAAAAACAACTTAACAAACCTAAACAAGGGTTCTTTTCCAAACTTAAACTAGAACCTCATAAATATCTGAGGGAAATCAGAAAGATGCAAGGGTTTGAGTTAGGTAAGAAGATCACCCCCCAGGAGTTGTTTAAGATAGGTGAATATGTTGATGTCACTTCACTCACCAAAGGTAGGGGTTTTACAGGAGCGATTAAAAGGTGAAACTTTAAGATAGGTCCTTTGGGTCATGGGGCGGGTTATCCCCACCGCTTTCAGGGTTCTGTGCAAGCAGGTAGAGGTGGTAGTAGTGCGCAGCGTGTTTTTAAGGGTAAGAAGATGTCTGGGCATTATGGTCATGAACAAGTTACGATCCAAAACCTCTTTATTGTTGGCTTTGATGAAATCAATAAGTTAGTGTTAGTTTCAGGCGCAATTGCTGGTCCTGAGGGTGGGATTGTTTTAATTAAAACTGCAAAAAAGAAAACTGGCAAGATAAAAGATATAAAGTTAGCAGTACAAACTGTTAAAGCCCCACAACTAAAAGCACCAAAAAAGCAGAAAACTAAGGTTGAAACCAACCAGGTTAACCCAAAAATTGAAGAAGAGAAAACTAAGTAATGGCTAAACTTAAAGTAATCCAGTTTGATGGTAGTTTTAAAGGTGAGATCCAACCTGCTAACCACCTCCTTTTAAAAAAAGCAGTGATCCAACCAGTGTTTGATGCTATCTTATTAGAACAAGCAGCATGTAGACAAGGCACTCACTCTACTTTAACTAAGGGTGAAGTTAGTGGTGGGGGTAAAAAACCATATAAACAAAAGCACACTGGTAAAGCTAGACAGGGTTCAATAAGAAACCCCCATTATGTGGGGGGTGGTGTTGTTTTTGGTCCTAAACCCAACCGTAACTACAAACTAAAACTAAACAAAAAGGCTTATCAACTTGCTTTAACTAGTGCCTTTGCACAAAAGCTTAACAACAACCAAGTGATAGTTGCTGAAGCCAAGTTGTTTGAACAAACCAATGCCAAAACTAAAAAGATGCTGACGTTTCTCAAGAATGCCAAACTAACTGAGCAAAAACTCTTGTTTGTGATTGATACTATCTCAAAACCACTGTTGTTGAGTACTAACAACCTAAAGCAGATAGTAGTCAAACAGTTTAATAAAGTATCAGTAAGAGATCTACTTTTAGCTAAAACTATCATCATTGAAAAAGCTGCTTTTACAAAACTGGAGGAACGACTTAAATAGGCTATGGATGTAACCAACATACTCTTAAAACCAGTCTTAACTGAAAAGAGTTATCTCAACCAGATGGGGGAATTGAAAAAATATGTCTTTGCAATTAACCCTAAAGCTACTAAAACCAAAGTAAAACTAGCGTTTGAAATTATCTATGGGGTTAAACCTTTAAAGATTAACACGCTAATTAGAAAACCAGTGACCATTAGAAATGGCACTAAATACCCTGGGTTTAGTAAGCTAGCAAAACTAGCAGTAATCACCTTACCTAAGGGAATGGATATTGCCATTACTGGTGAGAAAACAACCAAGAAAGAAACAAAGGATCAATAATGGCAATTAAAAAGATTATTAGTCGTTCTAACAGTGGGATTCACAACGCCACTGTCATTGACTTTAAAAAACTCCTTACCAATTCCAAACCCGAAAAGTCGCTTTTAGTTACTTTAAAAAAACATGCAGGAAGAAACAACCAGGGCAAGATCACTGTTCGCCACCACGGTGGGAGACATAAACGTAAGTACCGTTTAATTGATTTTAAGCGTTACCACTATGACAATTTAAAAGCAACTGTTAAATCGATTGAATATGATCCTAACCGCAGTTGTTTTATCTCCCTTTTACACTATCAGAATGGGGTTAAAACTTACATCATTAGTCCTGATGGGATTAAGGTTGGTGATCAAGTTTATTCATCTGATCATGCCATTGATATCAAACTAGGTTATTGTATGCCCCTTGCTTTTATCCCTGAAGGAACCCAAGTTCATAACATTGAACTTAACCCTAAGGGTGGGGGTAAGATAGCAAGAAGTGCTGGAAGTTATGCGAGGATCTTGGGTCAAGATGAGACTGGTAAATACATCATTCTCCAGTTAATCTCAGGGGAAACTAGGAAGTTTTTAAAGGAGTGTAGAGCTACAGTTGGTGTTGTCTCTAACTTAGATCATAACCTTGTTGTAATTGGTAAAGCAGGGAGAAGTCGTCATAAGGGAATCAGACCAACGGTTAGAGGTTCAGCAATGAACCCTAATGACCACCCGCATGGGGGTGGGGAAGGGAGAAGCCCAGTTGGCAGAGATGCACCAAGAACCCCTTGGGGCAAACGCCATATGGGTGTGAAAACACGTAACATGAAAAAACATTCAACTAACCTGATTATTAGAAACAGAAAAGGAGAACAATACTAATGTCAAGAAGTAGTAAAAAGGGCGCATTTGTTGATGCTCACCTCTTAAAAAAAGTGATTGAAATGAACAAACAAGCCAAGAAAAAACCAATTAAGACTTGGTCAAGAAGAAGTACTATCTTCCCTGAGTTTGTGGGTAACACCTTCAGTGTGCATAACGGTAAAACCTTTATTAATGTTTATGTTACTGATGATATGGTAGGTCATAAGTTGGGTGAGTTTTCCCCAACTAGAAACTTTAAACAACACACTGCTAACCGTTAGTTATGATTGCTTTTGCTAAACAATACAGAGTTCACATCTCCCCCCAAAAAGCACGGTTAGTGTGCCAGTTAATTGTGGGTAAGAAGATTAATGATGCGCAAAACATCCTTTTAAATACGCCAAAGAAAGCTGCTTACTTTTTAACTAAGTTACTAAATAGTGCGATTAGTAATGCCACTAATAACCACGGGATGAGCGGGGATCTTTTGTATGTATTTGAATGTGTTGCTAACCAAGGACCTAGCATGAAAAGAACAATCGCTAGAGCCAAAGGTTCAGGGAGTGTTTTAACCAAGCGTTCTTCAAACCTAGTTATTAAGTTATCTGATAATCCCAATGAAAGAAAATTACTCTTAACCCAACAAAAGGAACTGGTGAAAAAAAGAACAATGGGTCATAAAAAAGAGAAAGCAAAGCAAAAGCAAAAACAACAATAACTATGGGACAAAAAGTAAATTCAAACGGCTTAAGGTTTGGCATTAATAAGAACTGGATCTCACGGTGAACTGCCAGTTCCAACCAACAAACAGCAACCTGATTAGTACAAGATGAGAAGATCCGTAACCTCTTTTTTATCAACTATCGCAACGCTCAGGTGTCTAATGTTGAGATAGAAAGAACCCAAACGACTGTTGATGTTTATGTCTATGCAGCTCAACCTGCTTTATTGATAGGCAGTGAAAACAAAAACATCCAAAAGATTACCAAAATGATCCAAATCATTGTGGGCAGAAAGATTAAACTTGATCTTACTATCAATGAGATCGGCTCTCCGATGTTATCAAGTAGGATCATTGCCCGTGATATTGCTAATGCGATTGAAAACAGAGTACCACTCCGTTCAGCAATGCGCCAAGCTCTAACCAAGGTTTTAAAAGCAGGTGCTAATGGGATTAAGGTATTGGTATCAGGCAGATTAAATGGGGCGGAAATTGCCCGTGACAAGATGTATATTGAGGGCAATATGCCTCTTTCAACTTTAAGAGCAGATATTGACTATGCCTTTGAAAAAGCAAAAACCACCTATGGCATTATTGGGGTGAAAGTATGGATTAACAGGGGGATGATCTATGCAAAGGGTTTAAACAGAACCCCAGCACACATCCTCCATCCCCAAAAGAAACAGCTAAAAACCCCAACTATCAAAAAAACCAATTCAGTAATAGCAAAACAAAAACTCACTGGTAGTGATATTGAAACTGCTAGTTTAAAAGCACTTACTGATAATAATCAAAACCACGAATAGTTAAGATGTTACAACCAAAAAGAACCAAATACAGAAAACCACATAACGTCAGTTATGAAGGACACACTAAGGGCAATGGTTATGTTGCTTTTGGTGAGTATGGAATTGTTGCTACTAAGGGTAATTGGATCGATGCGAGAGCAATTGAATCAGCGCGGGTTGCTATCTCAAAGTGCTTGGGTAAAACTGGAAAGATGTGAATCAGGATCTTCCCCCACATGTCAAAAACCAAAAAACCCTTAGAAGTGAGGATGGGTTCAGGGAAAGGTAACCCTGAATTTTGGGTTGCTGTTGTTAAAAAGGGGACAGTGATGTTTGAAGTTGCTAACATCCCTGAACAACAGATGATCAAAGCCTTAACAAGAGCAGGCCATAAACTCCCTGTTACCTGAAAACTAATGAAAAGAGAGGAGAACAGTTAATGACAATCGCTAAGGAGCTGAAGCAAAAGAGCAACGAAGAGTTAGTGAAACTAGTAATTAAGCTTAAGGGTGAACTCTTAGAATACCGCTTTAAACTTGCCCATGGTGAACTTGACAAACCCCATCTGATTGCCAAGGTGAGAAAGTTATTAGCAGTTGTACTTACTATTCTCACTGAACGCAAACTCAACTGACAAGTTGAAAAAGATAAGTACAAGTTACTTTCAAGAAAAACCAATGAACTTATTGTTAACAGTTGAAAGCAAAAACTATCAACTAAACCTGAATCCAAACAAGAAACTAAAAAGGCTGAAGTTAAACCTAAGGTTGAATCAAAGCCTGAATCCAAACAAGAAACTAAAAAGGCTGAAGTTAAACCTTTAAAACAAGAAACTAAAAAAGTTGAAGTTAAACCTAAAGTTGAACCAAAACCTTTAAAACAAGAAACTAAAAAGGTTGAAGCTAGGATTGAAACTAAGACTAAAGTTGAATCAAAACCTTTAAAACAAGAAGTTAAAAAGGTTGAAGCTAAAAAATCTGTTTCAAAACCCCAAAAACCAGTTAAAGCCAAAATGATTAAAACAAAGGAGAAAAAACAATAATGAAGCGCAACCAACGTAAGCAGTTAATTGGCACAGTTGTTAGCACCAAAAATGCTAAAACAGCAACTGTCAAAGTAACATCACGCTTTAAACATCCTTTGTATCACAAATCAGTTATTCGCCATAAAAAGTACCATGTCCATAACTTTGGTGAACTTGTTGCTAATGATGGTGATAGGGTACAAATTATTGAAACAAGACCCCTTTCCGCTTTAAAGCGGTGAAGGATTGTCAAAATCATTGAAAGAGCAAAATAGTTTATGGTTAGTTTTATGACAAGATTAAATGTAGCTGATAATACAGGCGCTAAGCAAGTAGGTATTATCAAAGTTTTAGGTGCTACATACAAACGTTATGCATTCCTTGGTGATGTTGTTGTTGTATCAGTTAAAGATGCAATCCCTAATGGCATGGTTAAAAAGGGTCAAGTGTTAAGAGCAGTCATTGTTAGAACCAAAAAGGGACAACAACGCCAAGATGGTACCCACCTAAAGTTCCATGACAATGCTTGTGTGCTTATCAAAGAAGATAAATCCCCAAGGGGAACAAGAATCTTTGGACCAGTTGCTAGAGAGTTGAGAGAAAAAGGTTACAACAAGATTTTAAGCTTGGCGGTGGAGGTTGTTTAATGCAAAGGATTAGAAAAGGTGATAAGGTAGTTGTGATCACTGGTAAAAACAAGGGTGGTAGTGGGATAGTGCTTAAGGTATTAACCAAGCAAAACAAAGCGATTGTTGAGGGGATCAATAAGGTTACTGTTCACAAAAAAGAACAAGTCAACAAGCGCAGCAAACAAACAAACCCAACTACTAAAGAAGCCCCTTTACCATTAAATAAACTTGCTTTATTTGATCAGAAGGCCAAACAGCAAACAATTGGCAAGATCAAATACCAAATTGATCCTAAAACCAAACAAAAAACAAGAGTCTTTAAGAAGACTAATAATGCCATTTAACTGTTATGAATAACCTTGAAAAAACCTATAAAACTGAGTTAGTTAATCAACTCCAACAACAGTTGGGCTTTTCTTCCATTATGCAAGTCCCTAAGTTAACAAAAATCGTTGTTAACATGGGAGTTGGGGATGCAATTAGAGACAACAAGTTCCTTGAATCAGCACTAAATGAACTGCACCTGATTACTGGTCAAAAACCCGTTGCTACTAAAGCTAAGAATGCTATCTCAACTTACAAGTTACGTGCTGGCCAATTAATTGGTTGTAAAGTTACTCTAAGAAATAAAAAGATGTGATCCTTTCTGGAAAAATTAATCTATATTGCTCTGCCCAGAGTAAGGGACTTTCGCGGTTTATCACTGCGCTCTTTTGATGGGAAAGGTAACTATACGATTGGCATTAAAGAACAGATTATCTTCCCTGAAATTGTCTATGATGATATCAAAAGAATTAGGGGTTTTGACATCACTATTGTCACTTCCACCAACAAAGATAGTGAAGCACTTGCTTTACTGAGAGCACTAAAGATGCCGTTTGTAAAAGAATAGATATGGCTAAAAAATCATTAAAAGTAAAACAATCCCGTCCCAATAAGTTTAGTGTACGCGACTACACCAGGTGTTTAAGGTGTGGGCGTGCTAGAGCAGTGTTAAGCCACTTTGGTGTGTGTAGGTTGTGTTTCCGTGAACTTGCTTATGCAGGAGCAATCCCAGGAGTTAAAAAAGCATCATGATAATCAATAAAGTTCCCAAAGCCCATTTTGATCCAGTTTCTGATCTTTTCACTAAGATCAACAATGCTAGAAAAGCTAAGCTTTTAACTGTTACCACCATCGCTTCTAAGTTAAAGATAGCTATCTTAGAGATTTTGATTAAAGAGGGCTATTTAGCTAACTATCAGGTGTTGGAAAATAAAACTAAAACCAAAAAACTAGTTAGTTTCACATTAAAATACACCCAAAGAAGGATATGTTCTATTAATGGGGTGAAACAGATCTCAAAACCAGGATTAAGAATCTATCGTTCCTTTGAAAAACTTCCCCTTGTTTTAAATGGTCTTGGTATTGCAATTATCTCCACTAGTGATGGAGTGATGACTGATAAAGTAGCAAGGTTAAAGAAGATTGGTGGGGAGATTTTAGCTTACGTTTGGTAAAAAATTATGTCAAAAATAGGAAATAGATCAATCAAAATTGATCCTAGTAAAGTGAGTTTAATGCAAACAACAACACTGCTTACTATTAAAGGACCATTAGGGGAAAACACCATTAAACTACCCAAAAACTTACCCTTAAAGTTTGTTGTTGAAAATGACACTATTAAAGTAACTAATAACAACAACTTAAAACAAACTAAGATCTTACACGGTACTTTCAATGCGTTAGTTAACAACGCAGTTATTGGGGTTACCAAGGGTTTTGAAAAGAAACTCATCCTAGTTGGGGTTGGTTATCGTGCTAATGTGGAAGGGCAATTTCTCAACTTACAATTGGGCTATTCCCATCCTATTAAGGAGTTGATCCCAAACCAACTTACTGTTAAAGTAGAGAAGAACACTGAAATCACCATTAGTGGAATAAAAAAAGAGTTAGTAGGTCAGTTTGCCACTGAAATCAGAAAGTGAAGAAAACCTGAGCCTTATAAGGGTAAAGGGGTACTTTACTTTAACGAAGTAATTGTTAGAAAACAAGGTAAAACTGCAGAGGGCAAGAAATAAGATGACAAGAAACGATAAAAGAAGGATTAGACACAAACGGATTGTCAAAAAGATTAGGTTAACTAACCTTAACAACAGGGTTGTACTAATTGTTATCAAGAGTTTAAAAAACATCTCGGTTCAAGCTTGGGACTTTAGTAAGAACGTTGTTTTAACATCAAGTTCCTCACTTCAACTAAAATTAAAAAATGGCAACAAGGAGAATGCTAAACTAGTGGGAATGGATATTGCAACCAAACTCATCAAACTAAACCAAAAGGATGTGGTTTTTGATACTGGGGGTAGTAAGTACCATGGTAGGATTGCTGCTTTAGCAGAAGGAGCGCGAGCTAAGGGTTTAAATTTTTAAAGCTATGAATGATCAAAAAACTACTAACACTGGCTTGTTAACTTCCACTCTTAAAACCAAGCCCAAACACAACCTTAAACCTTCCAGTGAAGCCATTAAAAAAGCAGTGTCCAAAAAGGAAGGTCATTACAAAAACAAGCGCTTTCAAAAACATAACTTTAATAACAAAAGTGAGTTTGAAGAGAGGATTGTCAAACTCAAACGGATCTCCAAAACCACAAAAGGTGGGAGAAACATGCGCTTTAGTGTCCTTGTTGTTGTTGGTAACAAAAAGGGCAAGGTTGGTTATGGGATTGCTAAGGCATTGGAAGTACCACTTGCCATTAAAAAAGCGATTAAAAAAGCCCATAACTCCATTCATACAGTAGAGATCCATAAGGGTTCAATCTACCACGAAGTGATTGGTAGAAAAGGTGCATCTAAGGTGTTGTTAAAACCTGCACCTTTAGGAACTGGGATCATTGCTGGGGGAGCGATCCGTGCAATTGTAGAGTTAGCTGGTTTTAGTGATATCTATACCAAGAACTTGGGAAGAAACACCCCCATTAACATGATCCATGCCACTATGGATGGGATCTTAAAGCAACTCTCACCCAAAAAAGTGGCATTATTAAGAAATAAACCAATTAGTGATCTATAAAAACAATGGAACTACACCAATTAAAAAGTGTCTCTAAAAGCCGTAACCACAAGTCCAAAGTGGTAGGTAGGGGCCATGGCTCGGGATTAGGTAAAACATCATCACGTGGTCAAAAGGGACAAAAAGCAAGAAAATCAGGTTTAACTAGGTTAGGTTTTGAAGGGGGACAAACACCCCTTTACCGCCGGTTGCCTAAGTATGGGGTTGCTAACAAAGGGATCTTAAAAAAAAGGTGGGTTGTTTTAAATTTGAACAAAGTTGCTAAACTCAATCTCAAAACAGTTACTAGAGCAACTTTGATTGAAAAAAAGGTAATTAGTAAAAAAAATAACCTCCCTTTGAAGTTAATTGGGAACACAAAACTCACTACTCCCATCCACTTTGAAGTGCAAAAAATCTCCAAAAATGCTTTAAATGCAGTGCAAACTAGCAAAGGTAGTGTGAAAATTATCACCTAATGCAAACTGTTTCTTCACCCAAACAAAAACTTAACTTTGGTCAAAGGTTACTAACTCTATTACAGAACCGTGACTTTATGGTGTCGCTGGTTTTAACAGTGGTACTTTTAATCTTGTTTAGGGTGTTAGCAATTATCCCCTTACCAGGGATTAGGATTAATGAGAGTGTCTTGGATAGAAATTCCAATGACTTTTTTTCACTTTTTAACTTACTTGGGGGTGGGGGATTAAACCAGCTATCGTTGTTTGCAGTTGGGATCAGTCCTTATATCTCAGCCCAAATCATCATGCAACTGCTTTCAACTGATCTAATTCCTCCACTTTCAAAGCTAGTTAACAGTGGGGAAGTGGGGCGAAGAAAGATTGAGATGATCACAAGAATTATCACCTTACCCTTTGCTTTAGTGCAAGCATTTGCTGTGATCCAAATTGCTACTAATGCAGGCACTGGTTCAAGTCCGATTAGTTTAGCTAATAGTGGCAGTGAGTTTATTGCTTTTTATATTATTGCTATGACTGCAGGGACTTATATGGCAGTGTTTTTGGGTGATACTATCTCCAAAAAAGGGGTTGGTAATGGGATTACTTTGTTAATTCTCTCAGGGATTTTATCCCAACTCCCCCAGGGCTTTATTGCTGCTTACAATGTTTTGAGTGGGATAGTAATTACTCTAACCCCACAGTTAACTGCAGCAATTAGCTTCTTTATCTATTTCTTAGCATTCTTAGTTTTACTGTTTGCCACTACCTTTATCACCCAAGCGACCAGAAAGATTCCCATCCAACAATCAGGACAAGGGTTGGTTAGTGAAGTCAAAACCTTACCTTATTTGCCTATTAAGGTGAATGCTGCTGGGGTGATCCCTGTCATCTTTGCATCCAGTATTATGTCTATCCCTGTGACCATTGCCCAGTTTCAACCCCAAACTGAGTCACGGTGGTTTGTGGAGGATTACCTATCACTTTCAACACCCGTAGGGATCTTTTTATATGCAGTTTTGGTTATCCTTTTTTCCTTTTTTTACAGTTACATCCAGATTAACCCAGAACGGTTAGCTAAGAACTTTGAAAAATCTGGCAGATTTATCCCAGGGATTCGACCGGGCAATGATACAGAGAAACACATTGCGCGGGTGTTAATAAGGATTAACTTTATAGGTGCTCCTTTTTTAACTGTTATTGCTATTATCCCTTACATTGTTTCTTATTTCATTAGGTTACCTAACTCCTTGAGTTTAGGGGGGACGGGGATTATTATTATTGTTACTGCTGTAGTTGAATTTATCAGTGCACTGCGTTCAGCTGCTACTGCTACTAACTACCAACAACTAAGGAGAAACTTAGCAATTGAAGTGCAACAAACAGCTAAACAAGATAGTCTAGAGCAGCTTCAAAAAGAAGCACCAGGGATTGGTAACCTATGGTAGCACAGTTTAATAAGTTCATTATCTTAGGACCCCCAGGGGCAGGAAAAGGTACAGTTTGTAAACTGCTTAGCAAAACAACTAAGTTAGTCCATATTGCTAGTGGTGATCTGTTTAGAGAAGCCATTAAAAACCAGAGTGTTATTGGTAGAAAGATTGCAGCAATTATCAGTCAGGGTGGTTATGTTGATGATGCCACTACTAACCAGCTTGTTTATGAATATATCACTACCAATCCATTACCAAATGGTTTTATCTTAGATGGTTATCCAAGAACAGAGAACCAGCTTGATTTTCTAAATATTAAACTAACCATTGACATGGTCTTTGAACTAGTTGTTAGTGATCTGAATAAACTGATTACACGGATTGATAACAGGGTTATTTGTAACAACTGTAACAGTGTTTATAACTTGCTTTTTCAAAAACCACTAGTTGAAAATAGTTGTGATCAGTGTTCAGCTAAACTAGTGAAAAGGAGTGATGATAACAAAGCAGTGGTCAAAGCAAGAATGGAGTTATATCAACAAACAATTCAACCAATCCACACTTACTTTTTCAACAAACAACTTTTAGTACAAATTGATTGCTTTTTACCACTAGAAGAACAACTCAAGACAATCAAACAATTTATTAGATAAAGATGATCTATCTCAAATCTGCAAATGAAGTTGCAGGGATTAAAAAAGCATGTGCAATCTTCAAAGCAGTTAAGGCATATTTTACAATTGAAAAGTTACTTGGCAAAAAGTTGGTTACCATTGATCGTTTAATCAAACAATTCATTGAACAAAAACAAGCTAAATGTGCGTTTCATGGTTATCTAGGTTTCCCTGGTTTTAACTGTCTATCGTTAAACCAAACGGTTATCCATGGAGTTGCCGATCAAACTGTTTTTAAAGATAGTGATAAACTAACGCTTGACATTGGGATAGACTATCATGGTTATCTTTGTGATGCAGCTTTCACTTTACTTGGTAATAAAGCTGATCCAAAGGCAGTAAAACTGTTAAATGATGTTGAACAAGCATTTAGTAAGGTAATTGAACCTGAGCTATTTGTTAACAATCCGATTGGTAATTTATCCAATGCGATCCAAACTTACTTTGAAAACAAGGGCTATTTTCTTGTCAAAGAGTTTGGGGGTCATGGTTGTGGGATTAAGATCCATGAAGATCCTTTAATCTTAAACTGGGGAGAGAAAAACCAGGGCGTTAGGTTACAAGAGGGGATGGTAATCTGTATTGAACCGATGGTTATGACTGATAGTAGTGAGATAACAATGGCAGCTAACAACTGGAATGTACTAACTTTAAAGAGTAAGTTTAACTGTCATGTGGAACAGATGTATCACATCACAAACAACGGCTTTGAATGTTTAACTAACTAATGAAAAACGATAAACTCTTTCTAACAGGTAAGATACTGGAAATTATCCATGGTGATAAGTACCGGGTGATGCTTGAAAACAATGTTGAGGTTGATGCACATCTAGCAGGTAAAATGAAGATGAAAAGAACCAAGATTCTCCCTGGGGATGTTGTTGAGGTGGAATTTTCTCCCTATGATTTGAAACTAGGTAGGATAACCCAAAGAAAATAATTAAAATATTATGAAGGTTAGAGCAAGCGTAAAACCAATTTGTAAAGATTGTAAGATCATCAAACGTCACCGCATCTTAAGGGTGATCTGCAAAACCAAAAAACACAAGCAAAGGCAAGGATAATGGCACGAATCTTAGGGATTGATATCCCCAACCAAAAACGGATCGAGATAGCTTTAACATACATCTTTGGGATTGGTTTGTCAAGTGCAAAAACAATCTTAAAAAAAGCAAAGATTAACCCTGATAAACGCGTTAAAGATCTGAGTGAAGAGGAACTTGTTGCGATTAGAAACGCAGCAAGCGGTTACAAGATTGAGGGTGATTTGAGAAGAGAGATTGCTTTAAACATCAAACACCTAACAGAGATCGGTTCTTGAAAAGGGATTAGACACAGAAAAAACCTGCCAGTAAGAGGACAACGCACTAGAACCAACGCAAGAACCAGAAAAGGCCCTAGAAAAACAGTGGCTAACAAGAAAATTGAAAGTAAGTAATGGCTAAGAAAAAAAAGATTAATGTTCCCAGTGGTTTGATCCATGTCTCCTGTTCACCTAACAATACCATAGTATCAGCCACTGATCCCAGTGGTAATGTCTTGTGCTGAGCGAGCAGTGGTACAGTAGGATTCAAAGGTTTTAGAAAGAAAACCCCTTACTCAGCAGGGGTAGCAGCTGATAAGGTGGCTAAAACTGTGAAAGAGATGGGAATGGGGAGTGTTAAGATGTATCTGAAGGGAACAGGTAGAGGAAAAGACACCACGATTAGAAGCTTTGCTAATGCTGGGATTACGATCACAGAAATCAATGAAAAAACCCCTATTCCCCACAATGGCTGCAAGCCTCCTAAGCGTCCGCGCTAATCAAAACAACAACTTATGGAAAAATTTTTAAAGTACGAAATTAAGGTTAACAACAACCAACCAACCAACACTAACCCTAACTATGGGATCTTTGAAGTAGCACCGTTAGAATCAGGATTTGGGATTACCATTGGTAATGCGATGCGCCGAGTGTTACTTAGTTGTATCCCAGGCGCTAGTGTGTTTGCCATTGCCATTAGTGGGGTAAAACAAGAGTTTAGTAATGTGGAGGGTGTGTTGGAAGATGTGACTGAAATGGTGTTAAACTTCAAGCAACTAGTGGTGAGAATCTCTGATCTTTTGTTTGAAGATGGGGAGATGATCGAACCACCCTTAGAAAGGTGACCAGTTTTAAAAGTTACTGCTGAAAAAAAGGGTGCAGTATATGCAAAGGATCTTGAGTGTCCAGCTGGTTTTGAAGTGATTAATAAGGACCTTTATCTCTTCTCTTTACAAAAGGACATGAAACTAACAGTCAGTGTTTATGTTAAACAGGGTAGGGGCTTTACTAGCTTTCTTGAAAACAGAGAATTGATCAATTCGCTTGGCATTATTGCTACAGATGCTAACTTTTCCCCGGTTTTACACTGTGGTTATGAAGTTCAAGAGGTGAAAACTTCCAAACAAAAGTTAACTGACCATCTCACCTTTAAGATTGCTACTAACGGTGCAATTAAAGCAGTGGATGCGTTTGCTATGGCAGCAAAGATCCTAATTGAACACTTAAACCCAATTGTAAGTGTCAATGAGTCAATTAAGAATTTAACAATTATCCAAGAGAAAGCAGAGGAAAGAAAGGTGAAATCATTTGCCAAGCAAATTGAAGAACTTGACTTTACTGTTAGAACCTTTAACTGTTTGAAAAGAAGTGGGATCCACACACTCCAAGAGTTACTATCAAAGTCATTAACTGACATTAGAGAGATTAGAAACCTAGGTAAGAAATCAGAACGGGAGATTATCAAAAAGGTGCAAGAGTTAGGTTTAAAATTCCGTTCTTAATAAAATAGAGCTATGTCATACATTAATAAAGAGGGGAAAACCACAGCTTGAAGAGTGATGACAGTGCGTCAGCAAGTGAGTGCAGTGTTAAGTTATGGAAAGATTCAAACCACTTTAAAAAAAGCTAAGAACACCCAAAAAAGGTTAGAGAAGATTATTACCATTGCTAAAGTTGATAACTTTAACAACCGCAGGGCTGTTAAAAAGTGGTTATTAAATACCAATTCATTAGATGTAGATCAACTCACAAACCACCTTTTTAAAAAAGTAGCACCACGTTTTTTAAAGCGTAATGGTGGTTATAGTAGAGTGTTAAAGTTGGGAGTTAGAAGGGGTGATAGTACTGAAATGGCGATCTTACAGCTGATAGATGCTACCAACTAAACAAGCTGCTTGTAGTTTTATTAATGTTGCTTTTTCCTATAATGAACTGCCATTAATTAGGGAACTATCTTTTAGTGTTTATGAGGGGGAATATGTTTGTATTGTTGGTCATAATGGCAGTGGTAAATCAACCATTTCCAAACTGTTAACAGGGTTATTAAAGCCCCAGGCAGGTGAGATTAAGATCTTTGGTAAAACAGTTGATTTTGATAATGTTAGTTACTTGAGAAATAACATTGGGATCATCTTTCAAAACCCTGATAACCAGTTTATTGGGATCACTGTTGAAGATGACATTGCCTTTGGGCTTGAAAACAAGTGTTTTTCAAGACAGAAGATAAAAGCCATTATTGATGAAGTTACCCTACAAACCCAAACTGATGGGTTTATTAAACAAGAACCCCATAACCTATCAGGGGGACAAAAACAACGGGTAGCAATTGCATCTGTTTTAGCACTAAATCCTGCTATTATCATCTTTGATGAATCAACTGCGATGTTAGATCCTAAAGCTAAAAAAACGATTAAGCAGTTTATGGTTAAACTAGCCAAACAGGGCAAGTGTGTGATCTCAATTACCCATGATATGGAAGAAGTTACTAAAGCTGATAAGGTGTTAGTAATGAATGAGGGCAAACTGATCAAACAAGGTAAACCTGTTGAAGTTTTCACTAGTGAACAAGAGTTACAAAAAATCCGTTTAGACATCCCTTTTTCACTCAGTCTTTCAACCAAGATAAGAGGGATCACTAGTACAATTGATTACCAAACCCTGATTAAATCAATTGCCAAGCTGTGAAAAAAAAGATAGTCCCAATTAACCCTTTAAAAGCAGATGAGATTTTAGCAGTTAGTCACTTATCATGTGTTTTTAACAGTAAAACTAACAATCCCATTAAGGTGATTGATGATTTTTCCTATACCTTTCAAAAGAACCAAATTTACTGTATTATTGGTGATAGTGGCAGTGGTAAATCAACCCTTGTTAACCACTTCAATGGGTTGATAAAACCCAACCAAGGTGATATTTGGGTTAAAGATATCTATATTGGTGCTAAACAACGCAAGATTAAGAACTTTAAAAAACTGCGAAAAACTATCTCAATTGTTTTCCAGTTTCCTGAGTACCAATTGTTTAAAGATACCGTGGAAAAAGACATTATGTTTGGTCCAGTAGCATTAGGTCAATCCAAGTATGATGCGCGCCAAAAAGCGGCTTATTATCTGGAGATGATGGGGTTAAAATACCCTTTTTTAGAACGTAATCCCTTTGAATTGAGTGGGGGGCAGAAAAGAAGGGTAGCGATTGCTGGTATACTTGCAATTGAACCAGAAATTCTAATCTTTGATGAACCAACTGCTGGGCTTGATCCTGAAGGGGAAAGGGAGATGATGCAGTTAATTAAAACTGCCAAACAACAACAAAGAACGGTATTTATGATCACCCACCAGATGGAAAATGTCCTTGAGGTGGCTGATGTGGTTTTGGTTTTAGCTAAGGGTAAACTAGTAAAAGCTGCTAGTCCATATGAAGTGTTTATGGACCAAACTTTCCTTGAAAAAACAACGATTGTTCTCCCCCCTGTGATCCAAGTGATCAAAGATCTAATTGCGATTAATGCTCACTTTAATAAGTTAATTGAGTTGCAACCAAAGAACCTAGAACAGCTTGCATCAGCAATTAACAAGACTATAGCAAACCATGGATAACTTTATTAATGGCTATATCCCAAGAAACAGCTTTGTTCACAAGCTGCATCCAACTACTAAACTAGTAATCTTTTTACTGTTAGTTATCTTGGTATTTGTACCAATTGGCTTTGTTTTTCAAAGTGTTATCTTTCTTTTTGTTACCTTTGTTTTTTTTATTGCTAAACTCCCGGGGCGGTTTTACAGTTCAGCAATTAAGTCAATTACGCTGTTATTTCTCTTGTTGTTATTTGTAAACTGGTTTACCTTTCGTGATCCAGGGTTTTATCTTACTAGTGATCAACTTAACAGTTTACCAGCCATTGATAACAGCAAGTTTAGCTTTTGAAACATTAGTTTGTTTAACTATCAAGATAATGTTTTTTCCCAGGTTTTTGCTTTTAACAGGGGTAATTTAACCAACTTAAATCAACTTGATTTTTTCTATAAAGCTAACAATGCTGATAGTTACACCAAAGTAAAGGGCATTGATAGTTTAGCAAGTATGCTAGCAAACAATGGCAATGGTTTAAGCAAAGACAAAATTCTGAGTGCTTTTTTAGATCACAATTTAAACCTTTATTTAGCGAGAAGTTGGGGGGCAAATTTTGCTGGGTTTGTTGTTGATTTTAACCCAACAACCCAACTCTTTAAACTCACCCCTTTTCTAGCAAATGCTAGTTATGTTTTAACGTTAAGAGCAGTTATCTTAGCATTCTATGTCACCCAAAAGATCCTAATTATGATCTTATTTGCAACTGTACTCACTTCCACTTCAAGTTCAGTTGAACTAGCATATGGGATTGAAAGGTTATTATGACCTTTAAAACTCATTAAAATACCTGTTAATGTCTTTGCAATGACCATTGCCATTGCCATTAGGTTTGTCCCTTCCTTGTTACTAGAATCACAACGGATCTTAAATGCCCAAGCCTCCAGGGGTTTAGACTTTCGAAATGGGGGATTTTTAGTGAAGATGCGTTCACTCTCTTCGTTAGTAGTGCCAATGGTTTCCATTGCCTTTCGCAATGCCTCTGAACTTGCTAGTGCAATGGAAGCAAGGGGTTATCACCCTGCAAAGAAGCGCAGTAGTTATAGACAATACAAAATCACTTGGATTGATATTTTAGCGTTATTTTTGGTTTTTGCTTGGTTTGTTGTGATTATCTTTTTAACTATTAGAGGAGCGGTCTTTTTGGATCTAGGTACACCAGAATGGTTATTAACAGGAAAGATTAATGAACAGGTAATCAGGGATCTGAAGGTAAGTGGCTAGGTACTTGGGGATTGTTAGTTATGATGGCAGTTACTTTAAAGGGTGAGCGATTCAACCAAACCTAGCTACTATCCAAGGTTTATTGGAGCAAAGTTTTTCATTAATCATTGGCAGAAAGATAAAGGTAATTGGTTCAGGTAGAACTGATAAAGGGGTACATGCCATCAACCAAACCTTTCATGTTGATATTAATGGTGAAATTAATCTCAATTTGTTAATTAGAAAAATTAACCAGTTGATTAAGCCCCACTGTATAGTTAAAACCTTGGTATTGGTTAACGATAGCTTTCATGCGCGGTTTCAAGTTAAAACCAAGGTGTATGAATATCTGATTAACTGTGGGAATTTAAATCCGTTGCAATTTAACTATGTTTGGCAGTTAAACCAGCAATTGGATCTTGAAAAACTCAAAGCTGATGCCACTTTATTTTTAGGTAAGAAAAACTTTCTTAGCTTCAGTAGTTCGATTCACACTGATTCAATTCGCACAATTAGTAAAATTACCATACAAAAAGAAACTAACCAACTAGTTAGACTAACTTTTTTTGGCAGTGGTTTTCTCAGGAGTCAAGTGAGGATGATAGTTGCTTGTTTAGTGAATTTAAACACTAATAAAATGGCACTTGAAACAGTTGCAAAATTGTTTGAACACCCCAAGAAAGGGAGTTGTGTTGTTAAAGCCCCTAGTTGTGGTTTGTATCTGAAAACAGTGGTATATGAAAAATAGTTACAAGTGGGATCTATCAGTTTTATTAAACAACCAAAGCTTACAAGCAAATTTTTTAAAAATTCAAACAGTTAGTGAAGCGTTAATTAAAGCTTATAACAACGGGTTGTGTTTTACAAATAAAACTAGCTTTGAACAGTTTTTAGCAATCGATGATAAGTTCACTGAACTTGAAAATCGTTACACTAACTACCTTTATAACAAGCAGAATGAAAATAACTTGGATAAGGAGGTTAATGATGCAATTTTTGCATACCAGAGTTTTAAAAATAACCATAACCTTGCTTTCAGTACACTGCAACAGGAGTTATATAACCATGAAAAACTCATTAAGGATTATTTAACTGATCCAAAGCTAGCGGTTTACAAGCGCAACTTAATGTTAGTTTTTCGCGATAAACCCCACCAACTATCTAGTCAAACCCAGAGTTTATTGAGTCAAATTAACCCTTGTTTTAACCAAGCAGAACGGATTTTTAACATCCTTTCAACTGCTGATTTAAACTTGCAACCTGTTGTTTATCAAAACAAAAAATATCCGATTAACAGTGTGAGTGATTATCAGTCCTTACTTGAAAACACTAACAGGGGGATTAGAAAAGCTTGTTATGAGAAGTGGATTGAAATTTATTGAACTAATAGAAACAGCTTAAGTTTAAGTTTGGTGGAAAATTACATCCAACTAGAGAACTTCGCTAAACTAAAGAACCATCCTAGTTACATTGCCCAAACTGCTTTTAATGATGAGATTGAAGTTGGTTTTATTGATTTTGTTTACCAACAAGTAGCTCAGTTTGCCAAAACCTTTCAAGCATTTATTCGCTTAAAAAAACAGATTTATAAACATGTTTTAAAAGTCAATAAAGTTGAACCATATGATCTTACCCTAACACTTTTTAAAACTAAAAAATCATACACGATTGAACAAGCTAAACAAGATGCACTAAAAGTTTTAGATCTACTTGGTGACAACTACATCAAGATAGTTAAAAAGGCTTTTAATGAAAACTGGATTGATTGACTAGCAGATAAAAACAAGTACACAGGGGCATATTCAATCTCCAATGTTAAGGGCTTAGAGCACTTTTTTATCTTAATGAACTTTGATAAAACCAAATCATCACTAAATACGTTGGTACATGAACTTGGTCATTCAGTTCACTCTTGGTATGCATCACAACACCAATCCCAAAACATCGATCCTACTATCTTTTATGCTGAGATTGCTTCTATTGCCAATGAACTGTTGTTGTGTTATTATGAGCTGCAACTTTATAAAAATAACCACAAGCAGTTAATTGCTAGTTTATTGAGTCAAATCAACCATTTTTTTGGCGCTACTACAAGACAAATAATGTTTTCACAATTTGAAAAAGATACGCTTTATTTAATCAGAGTTAACCAGAAACCTGACTTTAAAACTTTGATTAAAATTTATGCAAATACTGCGGTTAAATACCAAGGTTTTAAACCTGAAGTAGTTGCTAATAAACTAAAAAAGACCCAGTATCAAAAATCATTGTCACACATCATTGCTATCCCCCATTTTTATGCAGGTAACTTCTATGTTTACAAGTATGCCATTGGCCAAGTTGCAGGTATTTTAGTAGCTAAAAAAATTAATAGTGGTGATAAAAAGATGAAAGATAATTACTTTAAATTCCTCAGTTCAGGTTCTAGTTTAGCGCCACTTGAAACCATTAAACTCTTAGGGATTGACCTTACTTCACCCCAACCCTGACAAGAAGCACATAACGAAGTAAAGCGTTGGCTTAAAATTGTGAAACAAAGCTTTAAAAAACTCCAAAAATAAGTGCACCATTTTAACCGCGCTAAAAAGGCCAAGAATAACGAGTTTTTTACTTTAATTGATGAGATTGAAAACGAAGTAATTAACTACCAAAAGCAGTTTGCAAATAAAACCATTTTTTGTAACTGTAATGATGGTAAAAATTCCCATTTTTTTCAGTTTTTTCAAACTAACTTTAACCAGTTACAACTAAAAAAACTCATTGGGTTTAGTTTTAATAATCTCTCACAAGCTGACAAGTTCACTTTTGATGGAAATAAAGTAACTAAAACCAAATTAAAGGGTAATGGTGATTTTAGTTCTGATGAATCGATTGAAGTGTTAAAACAAGCAGATATAGTTGTAACAAATCCACCCTTTAGTTTGTTTCAAAGTTTCATTGATCTGTTAATACAACACAACAAGCAGTTTCTGGTTTTAGGGTTAAATGCAGCAGTTAGCTATAACCATATTTTTACCTACTTTAAAACTAACAAGTTGTGGTTTGGCTATACCGTTAATAAAACAATGAGTTTTTCAGTTAACAGTGACTATCAACTTTATAACCCCAAAACTAGTAACTTTTTTACAAAAAATGGCAAGTGTTTCCAAAAGATAGCAGGTATCTCTTGGTTTACTAATTTAGGAAAACCACATTATAACCCCTTTTTAAATACCAACTGTTTTTATAAAAACAACGAAAAAAACTATCCCAAGTTTGATTGGTATGATGCTATTTATGTCAACAAGATTAAAAACATCCCTATGGATTGAAATGGATTGATGGGAGTTCCTTTAACCTTTTTAAACTGTTACAACCCCAAGCAGTTTGAATTAGTTGATTGTCTTGCTAACCCTTATGCTACCTTAGATACATTAAAAACAAATGCCTTTGTGAAATTAAATCAGGGTGATGTGAGAAATGTTAATGGTAAAAGAAGGTATGTAAGGGTAATAATTAAAAAACAACAAATTTAGTTTTTTCAACATTTAACAACTCTGTTTTTACAAAAGTTAGCTTAATTTTGCTAAATTGTTTTTTGATAGCACACTGCTGTCAACGAACCATGTTGATAAATGAAATTCAAACTATTTTTTTTATCAAGCAGTGTGTTGGGTCCAATTGCCCTATTTACAACTGCTTGTAGTGCTGTTTATAGGTTTGATCAAGTTGATGATGGCAAGATTAAACTAGCAACTGTAACTTCAGCTTCCGCTAGTGGCTCGCTTACTACTATCATCAGTAAATATAATTCACAAAAAGATCCTAATGATTATCCAGTGGAACTGGTTTCACTTGATAGTAGGGGCAGTTATTCTAATGGCAAAAAGGATCTGCAAGCTAAACTGCTAGCTAAGGATAAAAATAACTTTTATAACCTTACTTTTAACTATAGTGATGTAGTTTCAATCCTCTCAAGAAGCCAGATGGAGTTGAGCTTTGATACGGTTGATACTAGTAATTTTGATCCTAGTTTTCTTAGTTTTAACAATAATATTTCCAATGTTAATCCAAATAGCATCTATGCTTTACCTGCTACTGTATCAGGTGAAGTTTTAGTTTTAAATGGACCGGTGTTACATTACATTTTAAGTAGTGCTAAAAAAGATAGTAACACCACCCTTTCAACCCATTCAGCTAGCAATAATAGCAATAAGGGAACAATGGTTGTTGCTAGTGATAGTGAAACATCAAGTTTATGAACTAAATTAGAAGCTGCAGCAAAAATGAATGCTCAAACTAATGAAACCCAAGTTTTAAAAAGTAATTCATCAGAATCTAACCAAACCCAAGCTAGTGATACAGAGATTAAAAAGATTTGGGGTGATTATCAAGAAGTTGATGGAGGGTTGAAAAATTACACCTTTAAAGCTAGTGTTTTTAACAATTGAAAAGACCTAAATGACTTTGCCACCAGGATTGCAAAATCCTTTACAAAACTGCAAACTACCACTAAAAAAGGGGAGGAAGTACAAGCTGTATTTGGGATTGGTAGTTTGGAAAATGCCTTATATACTGCTTTATTTGCTTCTGGAAAAGCTGATTACAATAACTTTCTTTTTAACATCAAAAACCAGCGAATTAATTTCAGTAACTTTTTTAATAAATCCTCAACTGCATTTCAAAACCTTAAAACTATCTTTAACAGCTTCAAATCCTTAATTGATCAAAACGGTTTAATCTCAAATGCACACTTTAACACCCCAGTTAATGACTATGCTAAGTTTAACCAATTAGCTTTTTACACCTCTTCAACTGCACGCTTTCCCTATTCATTTGCAAGTGATAGTGTAAAGCGTTTAATAGTTAATGACAAGACAATTGAAAACAAAAACAATAAGAGTGTTTTTGAGGTTAATTTAAGTAGTGATAGTGATAACAACAGTAACCTAATTGGTACTGTATCACTGGAAAATAGTAAACAAGTTTCACTCTATGAAAAGCAAGTGGATAGTAATAAACAAATTGGTGTTGATGCTTTGTTAATCAAAGATGAAACTTTAATCAACCATCTTAAGAGTTTAAAATCGCAAGTTAGTGCAAAAAGTGCTAGTGAAACTTCCCAAACAAAACAAAACAAAACCTTTTTAGCATTTACAACTGTGAATGCAGATCAAAAAGCAATCTTTGATGTTGGTAAACTTAATGGCAAAACTGCCAAAATCATTATTAATGCTACTGAAACAACTAATGCTAAGATTAGCACCTTACAAGAAAAAGAGGCAATAGTTCTAAAAGCACCCCAACGCTTTGAGAGCACTGATCCATTTCCTATTGCTTTAGTGCAAGGTCCTAGTTTAATAGGGATCCATGCAAATGAAAGAGAGGATATTGAAACCAAAAAGTTTGTGAATTGGTATCTGAATACTAAAGTCCAATGGGAAGAAAATTCTATTAAAACTCCTGCAGAATATGTAGCTGATAAAGCTTCTTATCTTTTACCTTTTAAAAATAGGCTAAATAATACTAACAGTTACAATGAGTTTGTTAAAACTGCAGTTAGTCAGTTTGCTGATAAAAATGTAACTAAATTTGCAGAACCTGCTGATTTTTTATCCAACAAAGTACGTGATGGTGTTAAGAGTAATTTAAATGCTGCAATTAACAACCACAGCATTGATTTTGATAGTTTTATCAATGATTTAACAGACTATCTTGGTAGTGATGTTAAAAACATCTAATAATTTATTCCCCCAACCTCTATTTTCTGTTACTAGTGCCTAAGGTGGTAGTGGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAATCTTCTTCCTCTTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCGGTGAAGGATTCAAACAAAGACAGTGAGAAACTCGAAACAACTGCTTCATCGATGAGTAGTGGGGCTACATCTCCTCGCAAGGCCCTTAAGATAGAGGTGGAGAAAGGCAGTAATGTCAATCAAGGCGAACTAGCAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAGAACAGTAGTGGGACAGAGGTGAAGTTAGCTGCGAATGGGGAGTTTGCAGGAGACAAGGCCTGAAAACCATTGTTGACTACCGAGCAAATAAAAGATAATAGGGGGATGGGGGCTTTCTCCAACTCCCCCTCCCCTTCAACTTCCGCTTCCTCAACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACATTTAAATCAGCAAAACACCCGGTGGGTGTTTATACCTAACTTTTCACCTGACATCTGAACAGGAGCAGGTTATAGAAAAGCTAATAACAACAATAACGGCATTCCTTTTGAACAGGTGAAACCTAGCAATAGTAGTCAACAGTTTAATCCCAATTCAGATGATAATAAAGTCACTCAAGGTGGTGGCTCCCCAGCCAAAAAAACAACGTATGACAATTTACCAAACTCCATCAGTCCCACCAGTGACTGGATCAACGCATTGACTTTTACTAATAAGAATAACCCGCAGCGCAATCAACTGTTGCTCAGAAGCTTACTAGGAACTATCCCGGTCTTGATCAATAAGAGTGGAGGAAGTGGGAATGAGTTTACCCATACGAGTGAGCAGAAGTGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTATTATGTTTTTATCAAATCTTTATTTACTTTAATAGTTAAAAAAGTTTTGAATTTTTCTTAGTTTTTTATTTGTTTAATATTTAAGAAAGTCTCAAATTTTTATCAGTTTATTGGTCAAAGAAGTCGCAAATTTTCTTAATTTATTTATTCAATAGTTAAAAAAGCGTTAGGTTTTTCTTAGTTTTTTTATTTGTTTAATATTTAAAAATTTCTTGATTTTTATCCTTAATTTAATTAATAAAACCTTTACCCCTATTATCACAAACCATCCATAACACCAACCTGTTTGTGTTGTTCAAGTCTAGGGATGTAAAAGTTAAGTATGAGTCAAGTGGCAGTAACAACATTAGTTTTGATTCAACTAGTCAAGGTGAAAAACCATCCTATGTGGTCGAGTTTACTAACTCTACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAGTATCAGTTAGATCTACCAAATGTTACCAATGAGATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCCCTTACCAAGTATACCTTAAACAGTAGTTTGGCTAAACAAAAGGGTAAAACCCAAAGGGAGGTGCATCTCTCAAATTCAAATCAGTGACAATCGATGCGTAATCAACATGACCTAAACAACAATCCCAGCCCCAATGCTTCAACTGGATTTAAATTAGACAAAGGCAATGCATATAGAAAACTAAGTGAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACAGAAGCAACAACGGCAAAAAATGATGCGCCCAGTGTTTCTGGAAGTGGAACATCAGACACCGCTTCAAAATTCAAAAGTTACCTCAACACCAAGCAAGCGTTAGAGAGCATCGGCATTTTGTTTGATGGAACAACGGCGAGGAATGTGGTTACCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAAGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCGAGCAGCATGACCAACTTGTTAAACATGTTGTCTAGTAAAAACATCAAACAGAAGTTGGGGAAGGGGGGAACAGCAATGCAGGGAAGTTTCAGTGTCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATACAAATAGTGGAACAACTGGACCCATTAAAACTGCTTATCCGGTGAAAAAAGATCAAAAATCAACTGTCAAGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATTTAAATAATCACACCAAGTTAATTTACCCAAATTATTTACTTATTTATTAACCATTGTTACCCAATTTTTCAACCACTTCCCCACTCTATAAATTGTTATTTTTTAACAATTTGAACAATTAAATTGCAAAAAATAACTATATTAATGTAAATTTAGCTAACAAGCTTAACTGGTTGTTTTTGAGATGAAAGGGTTTTTAAAACCAAATTTCTCGCTCGGTGCTTTGTTTTTAACTTTAAGCCCCATAGCCACTGCATGCATTGCTGAAAAACCAGTTAACAACCGCTTTAACTTTAATAGCGAGCAATTAGCTAGGCTAAGAAAAGCAAGGGTTAACCACTGAAGAGATGGGGATACTTTGGAAGTTAGCTTTGCAAATAACCACCAAAAACCGATCCGTATCTATGCCATTGATACCCCTGAAAAAGCAGTTTTATCTATACAACGCAAATCAGAGATAGAACTTAAAGAAGCTAATAAAGCAACTGAGTTTGCCAAAAGCTTAATTCCCATTGGTAGTGAGGTGTGGATCTGACCACTAAATAGCTATAGCTATGATCGTGAAGTAGCTGCAGTGTTTTTCAAAACCAATCCATTGCAACTGCACTTTGAATCGTTTGCAGTTGAAATGGTAGCAAATGGTCATGCTTTACCTATTGCTGGTAATGACTTTGATTTTGTTTTTAGTGATTTGGACCCATTTAATCCCCTAAAAATAGTAGGGATTGAACTAGCTAATGGTTTAAACAATGCTTTTAACAACAGAAAAAACATCTTTAGTTATTTAGAAAACAGTTTTCAATCAATAACAATGGTCTATCAACAACGCGGTGTTGACCAAAGTTGAACAAGGTATTTAGCTCCTAGTAATGATTTTTCCTCTACTAAACTGGGGTTGGGATTAACCATCTATGAATTGAAACTAAACAATGGCTAACAATAAGAGTGCAATTGAGTTGAAAAACATCGTTGTTGATTTTGGTGAATCAGTTGCGATTGACAACATTAACCTTAGTGTTGAAAAACACCAACTAGTTAGCTTACTTGGTCCTAGTGGTTGTGGTAAAACCACTACACTTGCAGTTATTGCAGGACTTATTAAACCAACTAGTGGTCAGGTGTTATTTAATGGTTATGATGTCACCAAAAAACCACCCCAAGAACGTAAACTAGGGCTAGTTTTTCAAAACTATGCACTTTATCCGCACATGAATGTGTTTGAAAACATTGTTTTCCCCCTCTACAGTGATAACTCGTGAAAACAAGCAGTTTTGGAAAAAAACAGTGTTGCAAACCATGAGATTAACTGTTTGTTACTTACTAGCAACGGTGCATCAGTTCAAGAGATTGATCAGCTCAATAAGTTATTTCATGATAGTATTGAAAAACCCAAACAGATCCAATACCAAATTAATGACCTTAATGTTAGTGTTTTTAAAAACTTAAATGAACTAACTGCAAACCTTAAGTTAATACCAAGTAAGCACCAGTTTGCTATTACCAATCTCAACAAACAAACTCTAAAACAGATTAATGAACTGGAAGCTGAGTTTAAAACAAAGTGAAAGTTACAAAAACAAACCCCAATTAAGAGTGGGGTTGAACACAATGCCAAACTCCAAGCAATTAAACAACACTTTAGTTATGAAAAACAACGGTTAAAAAAACACTATTTCAAAACTAAAGTGGAACTAAAACAAACCCTTGTTGAAAACCTTAAGTTAGTTAAAAAAGCGATTAGTGAACAAACTAAGTTAATTAAACAGAGTAGTGATTACACTAAGTTAAAGCAATTAAAACGGTTAATTAAAGTTGAACCTAACCAACTCAAAAAACAATATAAGGTTTTTCTCAATCAGTTAATTAAAAACTATTCACTTAAAACTGATAAGTTAACTGATACTCAACTTAATGAAATTGAACAGATTAAAACCAGAATTGTTTCAATAAAACAGTTTATCAACAAAACTGCACTTGAAGTAGCTAACAAACTAGCGATTACCAAGATTTTAACCAAACGCCCTGATAAGATTTCTGGTGGACAACAACAACGCGTAGCAATTGCTAGAGCAATTGTCAGAAGACCTAAACTATTGTTAATGGATGAACCACTCTCTAACTTAGATGCAAAGCTAAGGGTACAGACAAGACAGTGGATCAGACAGTTTCAACAGGAGTTACAAATTACCACTGTTTTTGTCACCCATGACCAGGAAGAAGCGATGAGTATTAGTGATGTCATTGTTTGTATGTCAACTGGAAAAGTGCAGCAAATCGGCACACCCAGTGAACTTTATTTAAAACCTGCTAATGAGTTTGTTGCGCGCTTTTTAGGCACCCCTGAGATGAACATCATTGAATGTAGTGTCAAAAACAACCAGTTGTTTTGAAACAACCATCTGTTAGTTACTGAGAGTTTTAAGCTTAATGTAGAGAAACTCTTAGTTGGGTTTAGGTATGAACAACTAGTGGTCACTACTAACAAAAGTAGTTTGCAAGCTAAACTAATTAACATTGAAAACTTAGGTAAACACTTAGTTGCTACCATTAGTTTGTTTGATACCACCTTATCAATGCGCTTAGAATTGAATAGCCACTTAAAAGTAGGTGATAGTTTAAATTTCATTATTAAAGCTAACAACCTCCATTTTTTTGATATTGATACAAAACAACGGATTGAGATTTAACAATGTTTAAGTGGTTATTAAAACATCATAATCAACCTCATAGCCTCCAGTTAGGGTTACTAGACCAACCATTACCGTTCTGAAAGCCCTTTTTGTTGTTCCTCCCTGCGCTTTTAACAACAATTTTGTTTACCATTATCCCCTTCTTTTTAAGCTTACAGAAGGGTTTTAGTGCTAACAGTGATCTGTATGATCTCTCCTCCCAATCCTTTAGTTTACGAACCTTTCAGGATCTGTTTAGTGAATCTAACTTTGTGTTGGGCTTACGCAATAGTTTTCTCTATTCACTAATCTCTTTACCCTTTAGCATTATCATTGCTATTGTTATTGCCAGTGCCATAGTATTTGTGTACAAAAAATTGTTAAGAGGGTTTTGACAGACCGTGTTTTTTTTACCCTATGTAACTTCAGGGGTGGCAATCTCGATTGCCTTTGTATATATCTTTGATTCTGCCTCTGGTATTTTAAACACGGTGTTTAATGTCAACACCAAGTGGCTTGATTCAGGTTCACGTGATACATTTAATGCCTTGTGGGCTATCTTAATCTTTGGGGTGTGGAAAAACTTGGCATTTAATGTGTTGATTATCTCAACAGCAATGTTAAGTGTTAATCCCCAACTTTACAAGGTAGCAAGTTTAGATAGTGCCAATCCTGTAAGACAGTTCTTTAAGATTACTCTTCCCTCCATCCGTCCTACTTTAATCTTTCTTACTACCCTTTTAATCTTAGGGGGGATGCAAGTCTTTCCGCTGGCTTTGTTTGAAAACAAACCTGAAGAAGCGGTTGCTAATGGGGGGAATAGTATCTTGCTTTACATCTTTCAACAGATCCAAAGTGGCAATACTAACTTAGCAGGTGCTGCTACTTTAGTGTTGTTTGTGTTGGGAGTTTGTTATGGGTTAGTGTTACGTAATGGCTTTTATCTGATTGAATGGTTGCAGTGAAAAATTAAACAGCTTTATGTTCAAAAACAACTTACGCTTTACTAGTTGAATTAATCAACACAAGTTTTACCAACTTGATCTGAGCTTAAAAACCCGCTCGATCAAACAAATTGTTTTAACGTTGGTTTTCAAAACATTGGTATTGGGGTTTTTTGGGTTAATTGTCATCTTTCCCTTTTATCTGATGGTTGTGGTTAGTTTTGCTAGTGATGAAAGAGCATTAGACACAAGAACCCCAATCCTTTGACCTGATAGTTGAAACTTTGATAACTTTAGTAGGGTGTTAAGTGATGGGAAATATCTCAATGCAATAGTTGTCAATACTTTAGTAACGGTACTTTCAGTGTTACTAACATTGTTTTTTACCATTTGCATGGGTTATAGTTTTTCACTACGGAAGTGAAAATACAAAAAACTGGTGTGGTTTTTCTTTCTTAGTGTGTTAATACTGCCTGAGTCTGCGCTTTTAATTGGTCAGTATCGGATTGTAATAGTTGCTAACTGAAATAACCCCAACAGTCCCTTGATTGTTCTGGGACTCATTATGCCCTTTGTTAGCAGTGTTTTTAGTGGGTTTATGTACCGTACTAGCTTTGAAGCCATTCCATCTCAATTAAAAGAGTCAGCACTCATTGATGGGTGTAATGGCTTTAACTACTTTTTGAAGATTGCTTTACCAATGGTGAAATCTACCAGTTGAACAGTGGGGATTTTAACTGCATTTAGTGCTTGAAATTCCTATTTATGACCATTACTGTTGTTGGGCAACAGGGTGGATTTAAACATTAACTTGTGGGTGTTACAACAAGGGATCTTGGATGCTAACAGTAGTGATGAACAGATCAGAACGCTGTTAAATCTCAAGATGAGTGCAGCGATTCTAGCTATCCTTCCGATGTTTATTATCTACTTTTTGTTCCATAAAAGGATTATGAATGCCATTAAAAACAGAGCCAACACCATTAAGGGTTAATATGCAAAAGTTTAAACAACTGGTTGGTGCAATGCACAGATGGGTAAAACTAGCACTATTAGTAATCATTGTGTTATTAGGGATTATCTTTTGTCTGTTTGCCATCTATGACATTGCGCAAGTGATCATTACCATTATCAATGAAGGGGCACTTTTATAATCTTTGTTATGAAAAAAGGATCAATAACTGAAGCAATTAATGCCATTAAACAATTTGATAAGATTGTTATCTTTCACCATGTGCGCCCTGATGGGGATTGTTTAGGAGCACAACAAGGCTTGTTTCACCTCATTAAAGCTAACTTTAAAAATAAGGAGGTGAAGTGTGTTGGTAATAACAACAACCTGTTTAGCTTTATCAACATGACATTTACCAACCAAATTGATGAGAGCTTTTTAAAAGAAGCACTTGCCATTGTGGTCGATGCTAATTACAAAAACAGGATTGAATTGAGAGAACTGTTAGATAAAAACCTGTTTAAAGCAGTGTTAAGGATTGATCACCATCCCAATGAAGATGATCTAAACACTAGCTTTAACTTTGTTGAAGAAAGCTATGTAGCTTGTTGTGAGCAGATAGTGGAGATGGCCACAGTGGCGAAGTGGACCATACCACCAGTGGCTGCTACTTTACTATATATAGGTATCTATACGGATAGTAATAGATTTCTATATAGTAATACATCATATAGAACACTATACTTAGCAGCAATACTATATAAAGCTAAAGCTGATATAAGGATAGTACATGATCATTTAAACCATACTAGTTTAGCAGATCTTAAGTTTAAAAAGTATGTTTATAACCACTTTAAAACCCAAGGACAAGTGATCTATTTTATCTGTACTAAAAAGATCCAAAAGAGACTAAGAATGACTGCAGATCAATGTGCTAGAGTTAACTTGTTAAGTAACATAGCAGATTACAAGATCTGACTTTTCTTTATTGAACAAGCTAATAATGAGATCAGGATAGAACTGAGGAGTAATGGGATTAATGTCAGAGATATAGCCATTAAGTATGGTGGGGGAGGACATAATAATGCAAGTGGAGCGATCATTACTAACAAAAAACAAATTAGTGATGTTGTTAGTGATTGTGTGAAAAAAATTGTTTATAATTAAGTTTGTATGCACCAACCAAAGAAAAGACTGGCTAAGAAGTCTTGAGCCTTTCTAACCGCTGCACTTACCCTTGGGGTTATAACAGGTGTAGGTGGTTATTTTCTCTTTAACCAAAATAAGCAACGTAGTAGCGTGAGCAACTTTGCTTACCAACCCAAGCAGTTAAGTGTTAAACACCAACAAGCAGTTGATGAAACCTTAACCCCTTGGACTTGAAACAATAACAACTTCTCTTCACTAAAGATTACTGGAGAGAACCCAGGATCATTTGGATTAGTAAGAAGCCAAAATGACAACTTAAATATTTCAAGTGTTACAAAGAATTCTAGTGATGATAATCTCAAGTATCTCAATGCTGTTGAGAAATACCTTGATGGTCAGCAAAACTTTGCAATCAGAAGGTATGATAACAACGGTAGAGCTTTATATGATATTAACTTAGCAAAAATGGAAAACCCCTCAACGGTGCAAAGGGGTTTAAATGGCGAGCCTATCTTTGATCCTTTTAAAGGCTTTGGTTTAACTGGTAATGCCCCTACTGATTGGAATGAGATCAAAGGTAAAGTTCCAGTAGAAGTAGTTCAATCCCCCCATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGCATTAGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGAAAGCAACCCAATCATCCTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCAGTGAAGGATTCAAGTAAACAAGGGGAGAAACTCAGTGAAACAACTGCTTCATCCATGAGTAGTGGTATGGCTACATCCACTCGAGCCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCACTAAAGCATAAGAACAGTAGTGGGGAGGTGAAGTTAGAGGCAGAGAAGGAGTTTACTGAGGCCTGAAAACCATTGTTGACTACTGATCAAATAGCAAGAGAGAAGGGGATGGGGGCGACGGTGGTTAGTTTCTATGATGCACCCTACAGTGAAAACCATACTGCCTTTGGACTTGTTGATCACATCGATCCTAAAAAGATGGTTGAAAACTACCCACCAAGTTGAAAGACCCCGAAGTGAAACCACCATGGGATCTGGGATTACAACGCAAGAAACCTCTTGTTACAAACAACAGGGTTCTTTAACCCAAGAAGACACCCGGAGTGGTTTGATGAAGGACAAGCTAAGGCAGATAACACTAGCCCTGGCTTTAAGGTAGGGGATACTGATCACAAAAAAGACGGGTTTAAAAAAAACTCTTCTTCTCCAATAGCTTTACCATTTGAAGCATACTTTGCTAACATTGGTAACATGGTTGCTATTGGTAACTCGGTATTTATCTTTGGTGGTAATGGTCATGCTACTAAGATGTTTACCACCAATCCCTTAAGTATTGGGGTATTTAGGATTAAATACACTGATAACTTTAGTAAGTCATCAGTAACAGGTTGACCATATGCAGTGTTATTTGGGGGATTAATTAATCCCCAAACCAATGGCTTGAAAGATCTTCCCCTTGGTACCAACAGGTGGTTTGAATATGTACCAAGAATGGCAGTTAGTGGGGTGAAATGGGTTGGTAATCAACTAGTGTTAGCAGGAACACTAACAATGGGTGATACAGCTACTGTACCTAGGTTAAAGTATGATCAACTAGAAAAACACTTAAACCTAGTTGCTCAAGGCCAGGGACTATTGAGAGAAGACTTGCAGATCTTCACTCCCTATGGGTGAGCTAATCGTCCTGATATTCCTGTAGGAGCATGACTCCAAGATGAAATGGGCAGTAAATTTGGTCCCCATTACTTCTTAAATAACCCTGATATCCAGGACAATGTTAATAATGATACGGTTGAAGCATTAATCAGTAGTTACAAAAACACTGATAAGTTAAAACACGTTTATCCTTATCGATACAGTGGTTTGTATGCTTGACAGTTATTTAACTGGTCTAACAAACTAACCAACACTCCCCTATCAGCTAACTTTGTTAATGAAAACAGTTATGCACCAAACAGTTTGTTTGCTGCTATCTTAAATGAAGATCTGTTAACAGGGCTAAGTGATAAGATTTTCTATGGTAAGGAGAATGAGTTTGCTGAAAATGAAGCAGATAGGTTTAACCAACTTTTAAGTTTAAATCCTAATCCTAACACTAACTGAGCTAGGTATTTAAACGTAGTACAACGTTTTACTACCGGACCTAACCTTGATAGTTCTACCTTCGATCAGTTCTTAGACTTTCTCCCCTGAATCGGCAATGGTAAACCCTTTTCCAACTCCCCCTCCCCTTCAACTTCCGCTTCCTCTTCTACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACATTTAAATAAAGAAAACACCCGGTGGGTGTTTATACCTAACTTTTCACCTGACATCTGAACAGGAGCAGGGTATCGCGTTCAAAGTGCTAATCAGAAAAACGGCATTCCTTTTGAACAGGTGAAACCTAGCAATAATAGTACCCCCTTTGATCCCAATTCAGATGATAATAAAGTCACACCATCAGGTGGCTCCTCCAAACCAACCACCTATCCTGCTTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACTAATAAGAATAACCCGCAGCGCAATCAACTGTTGCTCAGAAGCTTACTAGGAACTATTCCGGTCTTGATCAATAAGAGTGGGGATAGTAATGATCAATTTAACAAGGATAGTGAGCAGAAATGGGATAAAACTGAGACAAATGAGGGTAATTTACCTGGGTTTGGGGAGGTGAATGGGTTGTATAATGCCGCATTACTCCATACCTATGGTTTTTTTGGCACCAATACCAACTCTACTGATCCTAAGATAGGTTTTAAAGCTGATAGTAGTAGTAGTAGTAGTAGTACACTAGTAGGTAGTGGGTTAAACTGAACTAGTCAGGATGTAGGTAATCTTGTTGTAATCAATGACACCAGCTTTGGGTTTCAACTTGGTGGTTGGTTTATTACCTTCACTGACTTTATCAGACCAAGAACTGGTTATCTAGGGATTACCTTAAGTAGCTTACAAGATCAAACCATTATCTGAGCAGATCAGCCTTGAACTAGTTTCAAAGGCAGTTATCTAGACAGTGATGGTACCCCTAAATCACTGTGAGATCCAACTGCTTTAAAATCCCTTCCAAATAGTTCAACTACCTATGATACCAATCCTACCCTCTCACCCTCCTTCCAACTCTACCAACCCAACAAGGTGAAGGCTTACCAAACCACTAACACCTACAACAAGTTAATTGAACCAGTTGATGCAACAAGTGCAGCAACTAACATGACCAGTTTGTTAAAACTCCTAACAACTAAAAACATCAAAGCGAAATTGGGGAAGGGAACAGCTTCTTCGCAGGGAAATAATAATGGAGGGGGTGTTAGTCAAACGATTAACACCATCACCACTACGGGAAATATTAGTGAAGGTCTAAAAGAAGAAACTAGTATTCAAGCAGAAACACTTAAAAAGTTCTTTGATAGTAAACAAAACAATAAGAGTGAAATAGGGATAGGTGATAGTACATTTACCAAGATGGATGGTAAACTAACTGGCGTAGTATCTACTCCCCTTGTTAACCTTATCAATGGCCAGGGAGCAACTAGTGATAGTGATACTGAAAAAATTAGCTTTAAACCTGGTAACCAGATTGACTTTAATAGGTTATTCACCTTACCAGTAACTGAACTATTTGATCCTAACACGATGTTTGTCTATGACCAGTATGTACCACTATTGGTTAACTTACCTAGTGGCTTTGATCAAGCTTCAATCCGCTTAAAGGTAATTAGTTACTCAGTAGAAAACCAAACCTTAGGAGTTAGATTAGAGTTCAAAGATCCTCAAACCCAACAGTTTATCCCGGTACTAAATGCATCAAGTACAGGTCCCCAAACTGTCTTTCAACCCTTTAACCAGTGGGCAGACTATGTCTTACCTTTGATTGTAACTGTTCCTATAGTAGTGATTATCCTTAGTGTTACTTTGGGATTAACGATTGGAATTCCAATGCACAGAAACAAAAAGGCATTACAAGCAGGGTTTGATCTTTCTAACAAAAAGGTTGATGTCTTGACCAAAGCAGTTGGTAGTGTCTTTAAAGAGATCATTAACAGAACAGGGATCTCTAACGCTCCTAAGAAGTTAAAACAAGCTACCCCAACCAAACCAACTCCTAAAACCCCACCAAAACCTCCAGTAAAACAATAAGATGAAAACAATGAGAAAACAGATTTATAAAAAAGCATACTGGTTACTATTACCCTTTCTACCATTAGCACTAGCCAATACCTTCCTTGTCAAAGAGGATAGTAAGAATGTTACTGCTTACACCCCCTTCGCCACCCCCATCACCGATTCTAAAAGTGATCTGGTTAGTTTGGCACAACTTGATTCTTCTTATCAAATCGCTGACCAAACCATCCATAACACCAACCTGTTTGTGTTGTTCAAGTCTAGGGATGTAAAAGTTAAGTATGAGTCAAGTGGCAGTAACAACATTAGTTTTGATTCAACTAGTCAAGGTGAAAAACCCTCCTATGTGGTCGAGTTTACTAACTCTACCAACATTGGCATCAAGTGAACGATGGTGAAAAAGTATCAGTTAGATGTACCGAATGTAAGTAGTGACATGAACCAAGTACTGAAAAATTTAATTCTTGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCCAAAGAGAAGGGCAAAACGCAAAGGGAGGTACATCTGGGTAGTGGGCAAGCAAATCAGTGAACCAGTCAACGCAACCAACATGACCTAAACAACAATCCCAGTCCCAATGCTTCAACTGGGTTTAAACTCACTACCGGCAATGCATATAGAAAACTAAGTGAGTCCTGACCAATTTATGAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACTGAAGAAAACGAAGCTAAAAATGATGCGCCCAGTGTTTCTGGAGGGGGATCATCTTCTGGAACATTTAATAAATACCTCAACACCAAGCAAGCGTTAGAGAGCATCGGTATCTTGTTTGATGATCAAACCCCAAGAAATGTTATCACCCAACTCTATTATGCTTCTACTAGCAAGCTAGCAGTCACCAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCATGTGGTACTGGGTGGTGGAGCGGAGTGCACAGGAAAATGCAAGTAACAAACCCACCTGGTTTGCTAATACCAATTTAGACTGAGGAGAAGACAAACAAAAACAATTTGTTGAGAACCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGCTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAAGGATCTAGTGACCAACGACACGGGGCTAAATGGTCCGATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATACAAATAATGGAACAACTGGACCCATTAAAACTGCTTATCCAGTGAAAAAAGATCAAAAATCAACTGTCAAGATCAATTCTTTGATTAACGCTACGCCCTTGAATAGTTATGGGGATGAGGGGATTGGGGTGTTTGATGCGTTAGGTTTAAACTATAACTTTAAATCTAACCAAGAACGTTTACCTTCCAGAACTGATCAGATCTTTGTTTATGGGATTGTCTCCCCTAATGAATTGCGAAGTGCTAAAAGTTCTGCTGATTCAACTGGTAGTGATACAAAGGTAAACTGATCAAACACCCAATCACGTTACCTCCCTGTTCCCTATAACTATTCAGAAGGGATCATTGATGCAGATGGATTTAAGCGTCCTGAAAACAGGGGTGCTAGTGTAACTACCTTCTCAGGGCTTAAATCAATTGCCCCTGATGGTTTTGCTAACTCAATAGCTAACTTCTCAGTTGGGTTAAAAGCAGGAATTGATCCTAACCCAGTGATGAGTGGTAAGAAAGCTAACTATGGAGCGGTTGTGTTAACACGGGGGGGTGTTGTTAGATTAAACTTTAACCCTGGTAATGATTCATTGCTTTCAACAACTGATAACAATATAGCACCTATCTCCTTCTCATTTACTCCGTTCACAGCTGCTGAGAGTGCGGTGGATCTCACTACCTTCAAAGAAGTTACCTATAACCAAGAATCAGGGTTATGGAGTTATATCTTTGACAGCTCCTTAAAACCAAGCCATGATGGTAAACAAACTCCTGTCACTGATAACATGGGCTTTAGTGTTATCACTGTCTCAAGAACTGGCATTGAACTAAACCAAGACCAAGCTACTACAACTCTTGATGTAGCACCTAGTGCACTAGCAGTGCAATCAGGGATCCAATCTACCACCCAAACCCTAACTGGAGTACTCCCACTTAGTGAGGAATTCAGTGCAGTTATTGCTAAAGATAGTGATCAAAATAAGATTGATATCTATAAAAACAACAACGGGTTGTTTGAAATTGATACCCAACTAAGTAATAGTGTTGCCACCAACAACGGTGGGTTAGCACCTAGTTACACAGAAAACAGGGTTGATGCATGGGGTAAAGTTGAGTTTGCTGATAACAGTGTATTGCAAGCAAGAAACCTAGTTGATAAAACTGTTGATGAGATCATCAATACCCCTGAAATCTTAAACTCCTTCTTTAGATTCACCCCTGCTTTTGAAGATCAAAAAGCTACCCTTGTTGCTACTAAGCAAAGTGATACATCACTTAGTGTCTCACCAAGGATCCAGTTCTTAGATGGTAATTTCTATGATCTTAACTCTACCATCGCTGGGGTACCTTTAAACATTGGTTTCCCTTCAAGAGTGTTTGCTGGGTTTGCAGCACTCCCTGCATGGGTGATCCCTGTATCAGTAGGTTCTTCAGTTGGGATCTTGTTTATCTTGTTAGTCTTAGGACTTGGGATTGGGATCCCAATGTACAGGGTAAGAAAACTCCAAGATGCATCGTTTGTTAATGTCTTTAAAAAGGTTGATACACTCACAACTGCTGTCGGTAGTGTGTACAAAAAGATTATTACCCAAACTGGTGTGGTGAAAAAAGCACCTAGTGCATTGAAAGCTGCTAATCCTAGTGTTAAAAAACCTGCTGCTTTTTTAAAACCACCTGTTCAACCACCAAGTAAACCTGAAGGGGAACAAAAAGCTGTTGAAGTTAAGTCAGAAGAAACCAAAAGTTAGTTTTTAACCTTTCAATAACCTAAAACACAATCTTTAAAACAAGGTTGTGTTTTTTTGTTTTTTGTCACTTTTCACTAAACTTGCAATTTAGAGAGTGGATATGAAAAGAACAGTTAAAAAAATAAAACCTGACCACTTGTTTAATAAAAAGCAGTGGCACTTACTGAGTGAAGAGATCAGTGATAACCCAATGATTAAGCGTTATTGACTCAAACAACAAAAGCGTCAGCTTGCCAAACTAAAAAAACGCCGTTATTTTAAAAATTAACTATGTATTCTACTTTAAGACAATATAAACCATTGAAAAGAAAGAAATGGTTAAACAAGATCTCAAAACGGAAGTTAAAACAACTTTATAACAAAGCTTATTAGTTTTGTTTTTTATTTTTACTAGTGCCTAAGGTGGTATTAGAGTACCACAAGCTCAGTAAGGATGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCTTTTGATCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATTCAAGTAAACAAGGGGAGAAACTCAGTGAAACAACTGCTTCATCCATGAGTGGTATGGCTACATCTCCTCGCAAGGCCCTTAAGATAGAGGTGGAGAAAGGTTCTTCAGGGTCTGACACCCTCACCAAATCCGACTTTGCTAAAAAGCCACTGAAACATAAAGAAAATAGTGGGACAGAGGTGAAGTTGGATGCACAGAAGGATTTTGCCGGAGAGAAGGCCTGAAAGCCATTGTTGACTACTGATCAAATAGCAAGAGAGAAGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAACAACAAACCCTTCTCCAACTCCCACACTGCTTCCCTTTCTGTTAGTTCAAATACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACACTTAAACAAAGAGAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCTGACATCTGAACTGGGGCTGGGTATCGCAAACAAGGTAACAATAATGGCATCCCTTTTGATAATGTGAAACCTAGCAATAGTAGTACCCCCTTTAATCCCAATTCAGATGATAATAAAGTCACTTCAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGAAGCAATGCATTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAACTGTTGCTCAGAAGCTTACTAGGAACTATCCCGGTATTGATCAATAAGAGTGGAACGGGAGATCAATTTAACAAGGATAGTGAGCAAAAATGAAACGAAACAGATAAATTAGGAGGCAACCTCCCGGGGCTTGGGGAGGTGAATGGCGGTTTTTATCAACTAAATAAAAACTTATTAGCTTATTTTTATTAGGTTTTTACTTATTTAATAGTTAAAAAAGTTTTGAATTTTTCTTAGTTTTTTATTTGTTTAATAGTTAAAAAACACTAGGCTTTACCTTTATTTAATTAATAAAACCTTTACCCCTATTACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCCAAGGATGTGAAGCTTACATATAGTTCAAGTGGCAGTAACAACATTAGTTTTGATTCAACTAACAACAAACCCTCCTATGTGGTCGAATTTACTAATTCCACCAATGTTGGCATCAAGTGAACGATGGTGAAAAAGTATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACGATGTACTGAAAAATTTAATTCTTGAACAACCCCTTACCAAGTATACGCTTAATAGTAGTTTGGCTAAAGAGAAGGGTAAGACACAAGTAGCGGTACATCTGGGTAGTGGGCAAGCAACTAATTGACGATCGATGCGCAACTCCATCAGTCTAAACAACAATCCCAGCCCCAATGCTTCAACTGGGTTTAAATTAGACAAAGGCAATGCATATAGAAAACTAAGTGAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATCAAGCGAATTGGAGTTCAACAGAAGAATCAACGGCAGCTAGTGATGCGCCCCTAAGTACAGGAGGGGGATCATCTTCTGGAACATTTAATAAATACCTCAACACCAAGCAAGCGTTGGAAAGCATCGGTATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTAGCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATACCAAACCACCAACACCTACAACAGGTTAATTGAACCTGACAAGTGACAATCAAATAGTGATTTGACTAACATGACCAGCTTGTTAAAACTCCTAACAACTAAAAACATCAAAGCGAAATTGGGGAAGGGAACAGCAATGCAGGGAAGCAAGACACCTTTTCCTTCGTTGTTCCTTATTCAGATAGTCATAGTAATCAAACTTCATCAGGAACCATTAAAACGGCTTATCCTGTGAAAAGTGATCAAAAATCATCAGTAGCGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGACAATAAAAAAACTACCTGTCAACAAAAAACAGGTAGTTAATTATTTATCCAATTAGTAAGTTGCCTATGGGTTAACAAAGAGATGCACTTAATATACGACCAACCTAATTTATTTGAAAGCGTATCTTTTATTAATGAATGTGGAACTAAGCAACTATTATTTAAACACAGCAAAAGCGTGTAATTTTTTATTAAAAGCGATTTAGTGGGAACAGAAATAAAAGATTAACATGACTTTATACAACTTCTGTTCCCATCTACTAAATTGCACAAAAAAAGCTTTTTGACAAAAAAATAGTTATTTTTTGTAATTGCTTAATTTAATAACAAACTAAACTATTATTGTTACTTAATGATTTTTTGATCTATATAGCACAATTAATATAACTTCATGATTGATCAAAACAAGTTAATTACTAAGTGAAAAAAAGCATTTGCAAAAGCTAAGAATTTAACTACTTTAGTTAATCTTAAGAACACTTTACACAACAGTGATTTAAAGCCATTACTCCAAAAGATTAAAACCGCTACAAAACTAAGTGAAAAAAGTAGTTTAGGTAAGCTTTATCAATCACTTGATATTCAACTAACTGATCTGTTAACTAGTTACAAAAAAACCTTTGAAATAAATAACCAAGTTAGTCAAAAACCTTCACTTGATGTGATGCTACCAGCAACAGAGTTTACCAATGGTTCTAATAACGCACTATATCAGGTTATTGATAATTTAGTTGAATACTTTAAAAGCTTTTTATTCACAATTAATTTTGATAGTGAACTGACCAGTATTAGTGACTGTTTTGATCTTTTAAATATCCCTAAAGATCATTCCAGTAGGAATGAATCTGATTCTTTTTATATCGATAAAACCAGTTTATTGAGAACCCATTGTACTGCTACCACGCTAAAAGCAGTCAGAACTTCTAAAAAAACTAATAATCCTGATATCAGGGTTGTCTCTTTAGGAGCGGTTTTTCGTAATGATAGTGATGATGCCACCCACTCCCATCAGTTTACCCAACTTGATTTTATGTGGATTAAAAAAGGGCTTTCATTAGCTAATTTAAAGTGGTTTATTAACAATATGATCACCCATTTCTTTGGGGAAAATACTTTTACTAGGTTTAGACTATCCCACTTCCCATTCACTGAACCCTCGTTTGAAATTGACATTAGGTGTTGGTTATGTCAAAATGGTTGTTCTATTTGTAAGCAAACCAAGTGAATTGAGATCTTAGGGGCGGGGATCATCCATCCCCAGGTGATGAATAACATGGGAATTGGGGATACTGAAAATATTACTGGGATAGCAGCAGGAATTGGGATTGAACGCTTAGCAATGTTAAAGTATGGGATTGATGATATCCGTGATTTTTATGATAACAACTTTAAGTTTTTAACCCAGTTTACTGACTAAAATATGTTGATATCAAAAAAAACACTTGGCGTTTTAATCCCTGACATCTTTAGTTTTTCTAATGATCAAATTGCCCAAAAGTTAGAACAAATGGGGATTGAAGTGGAATCAATTAAGCAGTTTAACAGCCCTGATTACCTCCAACTTGCAAAGGTTGTATCAATCCAACCCCATCCCCATGACAACAAGCTTTTTATCTGTGAATTACAAATTGATAAAAACAAGTTTATTAATGTTGTTTCCAATGCTAATAACATTAACAATCCTGATAATATCAACAAGTTTGTCATTGTTGCAAAAAAAGGAACTGAGTTACTCAACGGGTTAATTGTTAAAACCCAAAATATTAAAGGGATCATTTCAGAAGGGATTTTATGTAGCTATATTGACATTAACCCCTTCAGTAGACAGATCATTGAAAAAACAGAAGTTGCTGATGCGATTATCATTGATCATGTTAGCAATGATCATGACTGAAACCAATACCTCTCGTTTTTAAGTTTGGATGATGTGATCTTTGATGTTAAAACCCCAACTAACAGAGCAGATCTTCATAGCTTAATCTTTTTAGCAAAAGAACTTGGGGTACTTTTGAAAACCAAAACCTTTTTAAAACAAAAAAGTAGTGTTGTTAACCATGACTTTTTTAAGTTTCCCCTAAATTTAAAAAACAAGTTAAAAGCGAATTATTTTGGCGGTTTGTTCTTAAGACAAATTAACCAACATAGTTCACCTTGAACAGTTAAAGGACTGTTAATTAACCAAATGATCAAACCAGTTAACTATTATGTTGATAAAGCTAACTTAGTAACAGTGTTCACCGCTCAGCCAATCCATTGTCATGATGCAGATAGAATTGTTGGTAACATTGAACTTAAACAAGCAACCCATAATGAAACTTTTGTTGGACTTGATGACAAGCAATATGAGATTGAACCAGGGGATATTGTTGTTTGTGATGAGAAGGGCATTATTGCACTGGTAGGGATCATTGGTTCAAAGCGCACAATGGTCCAACCTACAACAACTAACATCTTTTTTGAAGTTGTTAACTGTAACAGTGAAACCATTAAACAAACTGCCAAGCGCTTTTTGATCAATAACTTTGCCAGTAAGTTTATGGTTAAACCGATTAGCTTATTAGCTACTGATAACTGTTTAAACTACTTACAAAACAGTTTACTAACCACTGATAACATTGGCAAAATTAGCCACTTTTCAAGTTCGCTTAAAGTTGAACCATTTAGTAAAAAGCTCACAGTGAATTTCCATAAGATACGCCAACTAATTGGCATTGAAAAAAAGGAACTAACTGATCAAACCATTAAAAAAAGCCTCAGTCAACTAGGGTTTAAAGTTGACAACCAACTTCTCAAAATCCCCAGTTACAGACAAGACATTAATACCTGACAAGACATTAGTGAAGAGATTGTGAAGTTAATTGATATCAATAAGTTAAAACCAATTGGGATCACTAGTAGTTTTAACTTTGAAAAGTCCAGTTACTTTAACACTTTTAATGCTTTAACAAAACTAAGAAAAAAGCTACAAACACTTGGTTTTCACAACGTTATTACCTACCAGTTAACTGATCAAAAAAGTGCAAAAACTTTTAATTTGTTTAACTTAGAAAATTTCATCACCATTAAAAACCCAGTGTCCCAAAACCATTCTGTAATGCGTGTTAGCTTAATTGATTCACTGTTAAAAGTGCTAAAAACCAATAACAACTATAAGAATGAACTGGTGAACATCTTTGAGTTTTCCTTTATTAAAACCCAAAACAATAGTGAACTGCACCTGGCAGTATTATGAGTTGAAAAACTGTTTACTTCTAGTTTCAATCCTATGCAAGGGATAAGCAATGATGTTTTTACTATGAAGGGATTAGCAAAACTCATTGTTGCTAACTTAGGGTTTAGTTGTGACTTTGAACCACTTGATGATAGTGACTATTTTGTTAATAATCAAAGTTTAAAAATAGTAGTTTTTAACGAACAGATCGGTTTTATTGGGCTAATTAAAGAATCATTGTTAAATAACTATGATCTGAACAATAAACCCATTTATTGTCTTGAAATCAACTTAGATAGGATGCTCTCTTCTCTAAACAGGATTGAAAAAAACTACCTTGGTTACAGTAAACTACAACCTGTTTGCAAGGATCTTACCTTTAGTTTTACCAACCCTGCTAGTCACTTTGATCAGTTTGCTAACATGATCAAAAGGATAACTGGCATTGAAAGTTGAAAGTTAATTAGTGTCTTTGAAACTATGCAAAACAACCAACTGATCACTAAGTACACCGTTCGTTATTTTCTGAAAAATGATGCTAACAAACCACTAACTAACCAAACAATTGAACTTATCACTAATAACTTAAAACTCCAGTGTGAAAAACTAAAAATTAAATTAGATATTTAGAATTACTTATTACTATCAAGATAGTTACCAACTATTTTGTTAGTAAAACTTAATACAATTGGCACAAAACGCAAAACACCCTTCTAAAAAAGAACAAAAACCACTCGTTAATGAACAGATTGCTTTCAATCAGTTCACTTTAATTGATGAAAACAGTACTAATTTAGGGATAGTTAAGATGGAAAACGCTTTAAAGTTAGCACAAGAAAAACAGTTAGATCTAGTTCTAATTGCTCCAAACCCAACCAAACCGATCGTTAAGTTGTTGGACTTTGGCAGATATACCTATGATTTAAAGCGTAAGAAAAGACAAGCCAAGAAAAACCAAACAATCATCCAAACCAAAGAAGTTGTTGTCAAACCAACGATTGCTAAACATGATTTAGAATTTAGAGCAAAACAGAGTAAGAATTGGATAGAAAAAGGTCATCATGTCAAGTTTATAGTCCGTGCCTTTGGCAGGGTTAGCACCAGGATAGAGTTAATTGAAAAGGTGTTTGATGACTTTTACCAGTTAGTTAAAGATGTAGTTGAGATCCAAAAACCTTTAACCGCTTCTTCCAAAACGATGTACGCTGCTCTATTAGTACCTTTAAAAAGATAGTTATGAAAACCAAAAGTGCTGCAGTAAAACGCTTTAAACTCACCAAATCAGGACAAATTAAGCGCAAACACGCTTATACTTCCCACCTCGCGCCCCACAAATCAACCAAACAAAAGCGCCATTTGCGCAAGCAAGCTACTGTGAGCAACAGTGAATTGAAAAGAATTGGTATTTTAATTTAGTTATGCGTGTTAAGGGAACAAATACAACCAGGATTAGAAGAAAAAAATGGTTAAAACAAGCTAGTGGTAGCTTTGGGACAAGAAAAGCTTCTTTTAAGGCAGCTAAACAAACTGTTATCCAAGCAAGCAAGTATGCTTACCGTGATAGGAGACAGAAAAAACGTGAGTTTCGTTCGTTGTGGATCTTAAGGTTAAATGCTGCACTGCGTGCACAAGGGATGACTTATTCAGTGTTTATCAATGAATTGAAAAAAGCCAAGATAGTCATTAACAGAAAGGTACTTTCTGAACTAGCAATTAAAGAACCTAATAAGTTAAATCTGATTATCAATACCATCAAAAAACCAACTAATAAACCAACTGTTGCAAAAACTTAGTAACGTTTTTAAAGTTAAGTTTTGCCACCTGTTTAAACTGTTCAATTTGACTAAAACCGCGTTGTTGGTTAAGAAATAACAACGCTTGTTCAATGCCATGACTACTGCCTTTGGGTAAATCATAGTTTACCTTTTGGTTGAGTAATTGCAGCTGATTAATAAAGCTATCTCTTGCTAAGATAGATGCAGCTGCAATCACCAGTGACTTAGTTTCACCATTAATTAGAAACTGATCAGGTAATAACACTGTTTTATCAGTGAAATTAGTTAGCTTATTTAGATAGTTAACAAATAACTCTTGATTGGCAAACTGGTCAATGCTAATTGTTACTGTTTGTCTTAATAACTGATTTTTTTCCAGTAACTTTTGGTAAAGCTGACAGTGTAAATTGGTCAAAAGTAAATTGGTATTTTTCAGTGATTTTGTCAAATCATTATACTGCTTGGGATCTAATGTAATTGTATGGTGATCCATTACTGTTGTTTGGATCGATTTAGCTAACAGTTTGACAGTGTGATCACTGAGTTTTTTTGAATCAGTTACTTGTAAATTTTCAAGATTAATAAGCTGTTTTTTTTCAATTAAAACAGCACTAACACAAATCCCACCAAAACTATCACCCTTGCCAGATTCATCACTGCCAATTAAATAAAAATCAGCTGGTTTGTAATGTTGCAACTATCTAGTTAATTAATAAAATTATACTTACTCTGATTGATTCTTAATGGCTGAACAGAAACGTGATTATTATGAAGTGTTAGGAATAACCCCTGATGCTGATCAATCAGAGATTAAAAAAGCCTTTCGTAAGCTGGCTAAAAAGTACCATCCTGATCGTAACAACGCGCCTGATGCTGCTAAGATTTTTGCTGAAATTAATGAAGCAAATGATGTTTTATCAAACCCCAAAAAAAGAGCTAACTATGATAAGTATGGTTTTGATGGGGTTGATGGTGAACCTGCTTTTAACTTCCAAGCAGATGTTTTTCAATCCTTTTTTGAAGAGATAGCAAAATCAGGGGTTTTTAACAACCAAACCAATCCTGAACAAAAAGAAAAAAAGAAACGTTACCACTGGTTCTCCAAAAAACCTAAGCAAGAACAACCTGAAATTAACTTAGATCACGTTGTTGAGCAAACCATTAAAAAGGTGCAACAAAACCAAAACCAAAACAAAGACCCAGATGAATTACGTTCTAAGGTCCCTGGAGAGGTTACTGCTAGTGATTGGGAAGCATTGGTTGGTGATACTAGGTATGGGTATTTTGATGAAACAGGGGATTGGAGTTGAAAGGGTTACTTTGATGAACAGGGCAAATGGGTTTGAAACGAACCAGTTGATTCTGAAACCAGTGAGGTATCAGTTGAACCTGAACCAACCCCAGTTGCCCCTGAAGCTAGTTTTGAAGAAGCTCAACCTGAAATTAATGCTGAACCAGAAGCTAGTTTTGAATCAACTCCAACTCCAGAACCAGTTGCCCCAGAAGCTAGTTTTGAAGAAGCTCAACCTGAACCAACTCCAATTCCTGAACCAATCCCAACCCCAGTTCAAGTTCAACCCCTGTTGTTAGATCTCAACCTCTTTACTATCCCAACTAAAGCTACTAAGGATGATCTTTTGTTTGACAACATTAACCTCACTACCTATGAACAAGTTGTTGATTATCTCAACAGTCAAGCAACCCCTAATTTAGCTAAAACCGATGGTGAATTGCAAACGATTGATGGTACCAACCCATTGTTATTAGAACAGTGCAAAAAGATCAAAAAACAAGCAGAACAACTCTTTAAAAAACTCTTTTTAAAAAAACAACTCCCCTTCATCACCCAACCTGAAGTTGTTGAGGAAAGTAAAACCAGTTTTGATGAGAACAACGTTAACCTTGTTTACTTTGAAAAGGTCCCTGAAATCCTTTTCATTAACCAACAACCTAAGGAGGTAAAATACACCCGTCAAGTCTTTGATGGGTTGACAAACAAAACAACTAGTGAAACGATTACACTAGAGATCCAACTCCTCCAAACCCCAAAAGAGACTGTTAGTGCCATTTTTAAAGGCTTTGGTAATGACCATGGCAAGGGCTGTGGGGATTTAAAGATTGTTTTTGAAAAGATTAAAAGCCCCTTTTTTCAAGTCAATGAGGATGGCTTGCACTCTGCTTGCATCATTGACCCTTTAGTTGCTTACAACGGCGGGATTATCGATGTGTTTGGGCCCTACACTAACTTCCAAGTTAAGGTAGATGGGGAGATAGACATCAATGCCATTATGAAGTTTGAAAAACTAGGCATTGCTAAAACCAAGCGCAAGGGCGATCTTTTTGTCCATCTCTATTACAGTAGTGTCCCTAAAAAGAAACTCACCACTAACCCCCAAGTTCAACAGTTCTTAGAACTTTTACAAGCTGAATATGAACTGTTGCAAGACAACATCAAGAGCTTAAAGTACTTTAAAAATAACCTAGTTATCCCCAAAAAGCCACTTGATCAACAAAGCTATCAATACCTCTCCCAAGAACCCATTAGTTAGAATTTGTTAATATGTGTGAAAAATCACAAACAATTAAAGAGCTTTTAAACGCCATTAGAACCTTAGTTGTCAAGAACAATAAAGCTAAGGTTAGTATGATTGAAAAGGAACTGTTAGCTTTTGTTAGTGAACTTGACAAAAAGTTCAAACAACAACTCAACAACTTCAATGAACTACAACAAAAGATCCCACTACTCCAAAAAGCTAACGAAGAGTTTGCTTTAAAGTTTGAAAGGATGCAACGCGAAGCACAAAACCAGATCCAAGCCAAACTAGATGAGTTGAATCTTAAAAATAAAAAGGAGTTAGAACAAGCCAAGAAATATGCGATTGCCAAAACCCTTGACCAACCCTTAAACATCATCGATCAGTTTGAAATCGCGCTTTCATATGCCCAAAAAGACCCTCAAGTAAAAAACTATACCACTGGTTTTACCATGGTACTTGATGCTTTTTCAAGGTGATTGGAAGCAAATGGGGTTACCAAGATTAAGATTGAACCAGGGATGGAATTTGATGAAAAGATTATGTCTGCATTGGAACTAGTTGATTCTAACCTTGCTAAAAACAAGGTAGTAAGAGTCTCAAAATCTGGCTATAAACTCTATGACAAAGTGATCCGCTTTGCATCAGTATTTGTCAGCAAAGGTAATAAAAAATCATAATAACAACTTATGAGTGAACAAAAAAGAAGAACAATCCAAATTGCGATAAGTGAAGACCACTATGAAGAGTTACAAAAGGCATTGGAACTACTTAAAGGGACCCAATTACCCTTTTCAACCACTGTTGAACAGTTTGTGGAGTTAATCTTATCTAACTATGTAGCTACTTCCAATAAGATTAGTAGTTTAGCTAAGAGTGGTTTTGATGTAGCTTCATTGCAGCAAGAACTTGAGAAGATAGGTAACCTTAGTGGGGTTGATGATAACCTCAAGGGTTTTCTCTCAGAACTGTTGAAAACCTCAAGGAATGGGTTTAGTAACCCCAATAAAGATGGCAAAAAAAATGATGACGATAATAACTCGTCATCAAAATCATAGTTATATAAAACTTTAAGTGGTGCCGAATAACAGAGTCGAACTGTTCTCTGATCCTTACCATGGATCTGTTTTGCCCCTAAACCAATTCGGCAGCAAAGCTAATTTCAATTTTAAATTCAAACTTTAGATGAAAAGTAACTACAGTGCAACTAACATCAAGATCTTAAAGGGTTTGGATGCAGTTAAAAAGCGTCCGGGGATGTACATTGGTTCTACTGATAGTAAGGGTCTGCACCACATGCTATGGGAAATTCTTGCTAACAGTGTTGATGAAGTTTTAGCTGGTTATGCAACCAATATTACTGTTACTTTAGATCTCAACAACACCATTACTGTTAGTGATGATGGCAGGGGTATTCCCTATGAGATCCACCAAGACAGTAACATCTCTACGATCGATACAGTTTTCACCTTTCTCCATGCAGGGGGGAAGTTTGATGATCAGTCATACAAACTAGCAGGGGGATTACATGGGGTTGGTGCATCAGTGGTCAATGCCTTAAGTGATCATTTAGAAGTAACAGTGAAAAGAAATGGTCAGATCTACCAATCAGTTTATCAAGCTGGGGGTAAGATCATCCAAAAAGCCAAAAAGATTGGTGATACAACTAGCCATGGTACCACTGTTAGTTTCCATGCTGACCCTAAGGTCTTTAAAAAGGCTCAATTTGATAGCAACATTATTAAAAGCAGGTTAAAAGAGCTAAGCTTTCTGTTTGCTAAACTAAAGCTCACTTTTACTGATCAAAAAACTAATAAAACCACTGTTTTTTTTAGTACCTCAGGACTAGTTCAGTTCCTTGATGAAATTAATAATACTGTAGAAACACTTGGCCAAAAAACACTGATTAAAGGTGAGAAGGATGGGATTGAAGTGGAAGTGGTTTTCCAGTTTAACCAATCAGATCAAGAGACAATCTTATCATTTGCTAACTCGATTAAAACCTTTGAAGGAGGGAGTCATGAAAATGGGTTTTGTCTTGCCATTAGTGATGTGATCAACAGCTATTGCAGAAAGTACAACTTACTAAAAGAAAAAGATAAAAACTTTCAACTTAGTGAGATCAGACAAGGGTTGAATGCTATTATCAAAGTTAACTTACCTGAAAAAAACATCGCTTTTGAAGGACAAACTAAGAGTAAGTTGTTTTCAAAGGAAGTGAAAAACGTTGTTTATGAATTGGTCCAACAACACTATTTCCAGTTTCTGGAAAGAAACAACAATGATGCTAAATTGATCATTGATAAACTACTCAATGCTAGAAAGATTAAAGAGCAAATCAAACAACAACGTGAGTTGAAAAAAAGTTTATCAAGTCCCCAAAAAGAGAAGATCTTATTTGGGAAGTTAGCACCTTGTCAAACCAAAAAAACCAGTGAAAAAGAGTTGTTTATTGTTGAAGGTGATAGTGCTGGTGGCACTGCTAAAATGGGCCGTGATAGAATTTTTCAAGCTATCTTACCTTTGCGCGGCAAGGTGTTAAATGTTGAAAAAATTAACAATAAGAAGGAAGCGATCACTAACGAAGAGATCCTCACTTTAATCTTTTGTATTGGTACAGGGATTTTAACTAACTTCAACATCAAGGACTTAAAGTACGGAAAGATCATCATTATGACTGATGCAGATAATGATGGCGCACACATCCAAATCCTCTTACTTACCTTCTTTTATAGGTACATGCAACCCTTAATTGAACTGGGCCATGTCTATCTAGCTCTTCCTCCTTTATATAAACTGGAAACCAAAGATAGAAAAACAGTTAAATACCTCTGGAGTGATTTGGAGTTGGAATCAGTCAAACTAAAGCTTAATAACTTCACTTTACAACGATACAAAGGACTTGGAGAGATGAATGCTGATCAGTTGTGAGATACTACTATGAATCCAACTACCAGAAAGCTAGTGCAAGTAAAGCTTGATGATCTAATTAACGCTGAAAAGCAAATCAACATCTTTATGGGTGAAAAGAGTGATTTGCGCAAACACTGGATTGAAGCCAACATTAACTTTAGTGTGGAAAACTAATGGATCAAAAAAACAACAACCTCTTTCAAAAGGCAATTGAAGAAGTCTTTGCAGTTAGCTTTAGTAAGTATGCTAAATACATCATCCAAGATAGAGCTTTACCTGATCTAAGAGATGGGTTAAAACCAGTACAAAGACGGATCTTATATGGGATGTTTCAAATGGGCTTAAAACCCACCACTCCCTATAAAAAATCAGCCCGTGCTGTTGGGGAGATCATGGGGAAATACCACCCCCATGGTGATAGTTCCATTTATGATGCAATTATCAGAATGTCCCAAAGCTGAAAGAACAACTGAACAACTGTTTCTATCCATGGTAACAATGGTTCAGTGGATGGGGATAATGCTGCAGCAATGCGTTACACAGAAACCCGCTTAAGCTTGTATGGATTTGAACTATTAAAAGACATTGATAAAAAGTTAGTTAGTTTTATCAATAACTTTGATGATAGTGAAAAAGAACCAACGGTTTTACCAACCTTACTGCCTAACCTCTTTATCAATGGTGCGAGTGGGATAGCTGCTGGATATGCAACTAATATTGCTCCCCATAACACTAATGAACTATTAGATAGTCTTTGCTTGCGAATAGACCAACCTAATTGTGAACTTAAACAAATTTTAAAAATTGTTAAAGGTCCTGATTTTCCAACAGGGGGTAATGTTTATTTTGAAAAGAGTTTAAGTGATATTTATCAAGCAGGCAAAGGTAAATTTATTATCCAAGCTAAGTATGAAGTTAACAAGAACTTAAACCAGATTGAAATTACCCAAATCCCTTATGAAACACTGAAAGCTAACATTGTCAAACAAATTGAAGAGATTATCTTTGACAATAAACTATCTGCTATTGAAAGTGTCATTGATAGTTCAGATCGCAACGGCATTAGGATCATTATTAAACACAAGGACTTTTTGCCTGCTGAGAAGATCATGGCCTTTTTGTTTAAACACACCCAACTCCAAGTGAACTTTAACCTTAATAACACCGTGATTGCTAACCGCTTTCCCATCCAAATTGGTTTACTAAGTTACCTCGATCATTTTTTAAAGTTTTGTCATGAACTAATTATTAATAAAGCTAAGTATGAACTTGAGCTTGCAAGCAAGCGCTTGGAAATTATTTTAGGACTAATTAAAGCGATTAGTATCATTGATAAAATCATCAAATTAATTAGATCAGCAGTTGACAAAAGTGATGCAAGAGAAAAGTTAATTGATAACTTTAAATTTACTTTTAACCAAGCAGAGGCAATTGTTAGTTTGCGACTTTACCAACTAACTAACACTGATATTTTTGAACTTAACCAAGAACAAAATGAACTTGAAAAAACTGTGATTAGTTCAGAGCAACTAATTGCTAGTGAAAAAGCAAGAAACAAACTCCTAAAAAAACAGTTTGAAGGTTATAAAAAGCAGTTTCACCAGCAACGAAGGTCACAAATATGTGGCTTTATTAACCAAAAAAAGGTGGAGGAAAGTGAGCTAATTGAAAACAAAACTTATGGGGTTTTAATCACTAAAGCTGGTAACTACCATAAGTTTGAATCTAACCAACTATTAAAAAGCACCACTGATTTTAAAAGTGAGAGTGACACAATTATCTTTGCACAAACTATTGCTAATACCGACCAAATTTTTATTGTCACTTCACTAGGTAACATTATTAATATCCCTGTTTATAAATTAGCTTTCAATTCCAAAAATAAACTAGCAAGTTTAGTTAGTAAAAAACCAATCCTTTTGGAGTATGAAACGATTGTTTTTGTTGGAACAATGAACAGTGTAAACCAACCAATCCTTGTTTTAACTTCCAAACTAGGAATGGTTAAACGGATTGATTTAACCAAACTTAACATTAAGCCACTTAAAGCTACTTTGTGTATCTCACTCCGTGATAAAGACCATTTAGTAAGTGCATTTTTACAACAAGATGATAAACTGATCTGTTTAGTGTCTGATCACAACTATTACACTGTTTTTCACACCAATGAGATCCCATTAATTAGTAGTAAGGGGATGGGAGTGAAGGGGATGAAGTTAAAACTAGAGGATCAAATTAAGTTTGTTGTTGCTTTTGAAGCTAATGAACCGTTAGTGATGATATGTAGTGATGGTAGTGTCATTAACTTAAAACAAACTGAACTAGTTGTAGTTAGCAGGATGGCAACTGCAAAAAAACTGCCTGTTAAGAAAGCAATTAACTATTGTTTTAGTGATGCAACTAACACCCAGTTAATTAATTTTCAGGGTAAGAACGGTAGTAAATTAATTACAACTAGTGAACTGAACCAGATGAGTAAAACTGCAATTAGTCAAACCAGGTTTAACAAACTTAATTAGGTTTGGTTGTGGATCTTTTTATCATATTTTTCTATCTCTTCTTTTAGAGTAGTTAATAAGGCTTTAATCTTTTGGTAGTCCATTCTGGTTGGTCCTACTATAGCAAGTTGGTGCTTGGCTTCACTGGTGGTATTGATTAAAGTAGAAGCAACTGATATCTCTTTAAAACCCAATTGATCACCAAAGACAATGTTGGTCTTTTGGTTGGTTTGGTTTATGAATGCCATCTGTTGTCAAACACTAGTATCTTCAAGCAGATTTAAGATTTTAGTGAGTTTCTCTTGGTTAGCAAACTCGGGTTGTTTAGCAAGGTATTGGATCCCATAGATCTTTTTATTTGCCTCAATTTGATCTAAATCAAATAACTTAAATAAGATCTCATCAATTACATACTGATACTCATGAACCTTGGTTCTAATGATCTCTTTTAAAACTTCTAGTTGGTTGTTAATCTCTGAAAAGCGGGTATCAATGATGCGATCATTAAAGATCCTTACACAAATAACAATGTCTTCAAACTGCCTTTGGTTAGCATAGCTAATGGTTTTTTTAAAGACTTTACCACTAGCGGAGACCAATAAAAATAAGGCAAATGACTTATCAAGGATGATCAGATCAATCTTTTTTAAAACTTCATCACTACTAAAGTTAGTTAACACTACTGGTAGGTTAATGATCTCATTGATAAACTTAACCCCAAGTTCAATCACCTCATCAATCGTTTTGTGTTGCTGGAGAATAACACTTCTTAAGCGGGTTTTCAGATCGTTAGTGGTGTTACTAACATTTAACACTTTGACATAATACTGATAACCAATTTGGGAAGGAACACGGCCTGAAGAGATGTGGTTTTTCTTTAAGAACCCTTTTTTCTCTAAAGCTGCCATCTCATTTCGTAATGTTCCCCCAGAGAGGTTTTTAAAATATTTTTTGGTTAGTAATTTACTGCCAACAGGGATAGCATAAGCAATGTATTCATTGATAATTGCTTTGAGAATTTGGGCTTGCCGTGGCGTTAAATTCTTCATTAGTTATCTAATTTTATCTTTTAACAATAACCAGCTTGACCACTAATCTAAAACAAAAGTTAAAAACTGCACCTAAAAAACCGGGGTGTTATTTGTGAAAAGATAGTAACGGTAAAGTTTTATATGTTGGTAAAGCTAGTAATATTTTCAACAGGGTCCACCAGTATTTTCAAAAGAATAATCCTTATAAAACCCAGCTATTATCAAGCCAAATTAGTGATGTTGATTTTTTCATTCTCAAAGATGAAAATGATGCTTTAAATCTGGAAGCAAAGCTCATTAATCAATATCAACCCCGCTTTAACTTAGTTTTAAAACAAAACAATGGTTATCTTTATTTTTATATCACTAAAGCCAAAAAACCTACCTTGGAATTAGCCAGAAAATACCAGATTAAAACCACTAAGTGCTTTGGACCGTTTGCTTCAAGTAAGTTTAAGTTACGTGAGATCCATGACCTACTTTTAAAACTCTTTCCTTTAAGAAAGTGTGCACCTCACCAAAAAAACCACCCGTGCTTTTATTTTCAGATGGGTTTATGTATGGGGCAATGTATGCAAACTGATACTAAGGAAAAATACCAACAGGTAATTAGTAACATTGAACAGTTTTTTAATGACCCTAGTGTGGTAATTAACTATTTAAAAGCTGCAGAAAAAAAGGCAAGTGATAATCAGGAATTTGAAAAGGCCCAGCAGTTTCTAACACTGCAAAAAGCAGTTTTAGAGTTAACAAAAACCCACCATACCACTATCATTAAACAAAAATCAAGCCATGATTTTATTGGGTATGTCTTTCAAAATAACGTTTTGGCCATTACCATTTTTTGTTATGAAAAAGGGGAGTTAACTGATAAAGAACAAGCAGTGTTTACCCTAGAGCAAACTGACATTGTGGAAGTTGAAAGTGCTATTATCACCTTTATCTACCACCACTATAAAACTACCCCACTTCCAAGTAAGATTACTGTTTCACTTGATGAAACTAACCTAAAACTTATTAGTGATAGCTTAAAAATTGGTGTTTTTAAGCCCAAGAATGGTAATGAAAAACTGATCTTACAAACTGTTATTGATAATGCCAAACATGCACTTGCAACCAAGTGGTTGAAGTTTACTAGTAACTATGATAAAACCCAGCTCCACAAGGATTTAGCACAACTTCTAAATACTGATTATATCCATAGTCTTGAGATTATTGATGTGTCATTCTATGATCAAAACCATGTTGTTGGTTGCATGTTAAGGTTTGAAGATGGTAAAAAGATCAAACACTTATCAAGAAGATACAACATTAACAGTTTAAAAAAAGGTGATACTAACCACATTGCTTTACTTGTTTACAGAAGGATCTTAAGTGCGATGCAAACCAAAGCTAACCTCCCTTTTAGTGATCTTTTAATTATTGATGGTGGTAAAGCACAAATTAAAAGTGTTAAGCAAGTTTTTAGTCTCTTCAGTAATGTTAAACCACCCATTATCATTGGACTAGTTAAAAACAAAAACCACCAAACTGATCACATTATGTTATCTGATTTCCAAGTTAAAAAGATAGCAATTAACTCCCCACTCTTTCACTATTTAGCAACAATCCAAACTGAAGTTGATGGTTTTGCTAAAAGAAGTGCTTTTAATAAGTTAAGTAACCACCAACTGCAAAACCCGTTGCTACAAATCCCAGGAGTTGGCAAGATAACTGCCCAAATTCTCTTTGATAACTTTCAAACGCTCAATAACATAAAATTAGCTTCAGTTAATGAGTTAAGCCAGTTTATTAAAAAACCATTAGCACAAAAGATTAAAACTTACTTTGCAAAACAAACTGATTAAGGTTTTGGTAATTGCTGATACCCATGGTCAAAACCAGAGGTGGATTGAACTGAAAAACTACCATAACCCTGATGTGATTATCCATGCAGGAGATCACATGACCACTAAACAATTCATGGATCAAAATGCTACTTTTTGAGTGGCAGGTAACAACGATTCAATTGGCAATGAAATTGAAATTTTCCAGTTAGGGCAAATTAACTTTGTGTTAATGCATGGTCACCAAGCACCAAGAGATAACCTGAAAAAGTGGTACCAATTATTAGTTTTAAAAGCACAACAATACCCTTGTGATGTTTTAATCTTTGGTCATAGTCACATTGAATATACCAACAAAATTAATATGATCCAGTTAATCAACCCTGGTTCTCTACAACTACCAAGAAACCAAACCAACACCCCTTCATACTGTACCTTTATTGTCAATAAAGACGAGCTAACTGATCTAACTATCCACTATTACCAAGCTTCAAAAGTTAGTTAACAGGATCTTTTAGATAGATAGGTTGTAAGGTTAGAGGATCTTCGATTCTTTCAAAGTGATCGATGTTGCTTAATAACAGCTTGCTATAACTCTCAATGTTTTCAAAGTTTTCATACATAGGAAGTTCATTGTTTGCCTTACATAACTTCACAAAATCAGCTTTACTAATTAGCTTTATTTCACTAGTAGTTTGGCTGTATAGTCCACAATAGTTCTGATCATTACCACAACTAATCTTGCTAATCCCATGTTCATATGGGATTTGAAAGCGGAGTGAATTTAAAGCATAAAGTTCACAACTGGGATACAATAAACACCAGCTTTTAGCAATGATAGTTGCTATCCTCTGACCGGTGAAACTACCAGGGCCAATTGTGACATAAAACTGTTTAATACTGCTTTTTTTCAACTTGTTTTTTGTTAACATTGTCTCCAGATAATAAACAGCAAGTTCAGTTAAGTTCTGTTCAACACCAATGGACAACTCATCAACCACTGCATTGGTTTTCATCTCTAAAATAATGATGTTAAGGGTTTTATAAGCACAGTCTAAAAACAGCTTATACTTACTTAAAAAGAACATGGAGTGGATCGGTTAAAGATAAGTTTAAACTCTTAAGTTTCGTTGAAAACTTAGGTTCAAGTGGGGCGTGAAAGTCCATAGGTTTGTTTAAAGTGGGATGGATAAAACAGATCCTGTTTGCATGGAGAAACTGACCATAGCTATCTTTCTTTTCACTTTTAATTCCATACAGTGGATCATTATAAACTGGATGTTGGATAAATTTCAGATGCACTCTAATTTGGTGGGTTCTACCTGTTAACAATTCCAAGCTAATTAGTGCTGCTTTTTCATTCTGATTAATCACTTTAAACTTAGTTATTGCTTGCTTTGCTTTAGCAGTTTGGGCTATTTTAAACATTACCTTGTTGTTATTAACCCTTGCTAAAGGTGCATTAATTGAACCAGTTAAGGCATTAAAAGGGAAGTGGACTAGTGCTACATAATAACGTTTTAAGGTGCGATTTTGCAGTTGATTTTGCAAATTTAATAAGCTTGCTTGGTTTTTACAGACAACAATTGCCCCACTAGTATCACGGTCCAATCTGTGCACTAAGTAAACAGGGTTTTTGTTGTTGTGAAAGATACAAGCAGCTAACAAGCTGGCTTTTTCATTGAAAGTGGTGGGATGGGTTAACAAACCTGATGGTTTGTTAATAACCATCAAATCCTTGTCTTCAAAAAGAACCTCAAGCTTTAAGTTATAAGGTTCAACACTAGTAATGAAATCACTAGTTGTCTCATCATGAATCTCAACTTTAATTACATCATCTTTTGCAACTATTAAACTGTTTTTAAAAGTTAGTTTTTCATTAACTTTAATCTGTCCATTCATGATCAGCTTCACTACCTTTACTCTTGAAAGGTTCAGTAAGCTAGCTAAAAGACTATCTAAGCGTTTGGTAGTTGTAACAACAAAACACTGTTTCATTACTGGTTTTTGGAATGTTTAAACTCTTTGAACATCTGGATTAAAAAACAAAAGAATAAACCTATAAAACCAAAGGTAATACAACAATCAGCAAAGTTAAATACTGAACTACCATTCTGAAAGATAAAGTAATCTAACACTGAATCATTAGCTGAAGTTAAACGATCAAAGAAGTTTCCAAGTGAACCAAATGCTAAAGTTGTAATTCAAAAGATGTAACTATATTTCACCATAAAAACAAGAAAAACTAACGCAATTACTGATAATAATCCCTGGAGAAAGTAAACTAAGCCAGTTTGGTTTTGTAATAAGCTAAACCCTACCCCTTTGTTTCTAATCACATAGATATTAATAAAACCACTATTTGCTACCATGGTGTTATCCATCTCACCATTTAACGCATTTCTTAATATGAAAACTTGCAGTAAGATAATAAAGCCAACAAACCCAATCATTGTCAGTTTATAGAATAAAAATGGTTTTTGGTTTGCAGTTAAAACCTGGTGTTTAAGTTGTGAAAAAAACTTGGTTTTTCTTAATTTCATCTACTTGTATATTCAGTTAAAATTCACTAATTAACTAACAAAAATTATTAAGGAGTATTAACACTTTTTTACTAGTTTTTAAACTAACTTTTTTAAGTGTAATCCTAGTTTAATTTCTGGTAGTTTTCTTTACTAATAGCTACTTGTAAAAAATTAAAAGTTAGAATTAATGGCACTATTTAGTATATAGATATCAGGCTTTGGGGAAAACAAAAAACAAATCAGACTGACAGATCTTTCTAGAAGATTACCGTTTTTATTTTGAAACAGATTTTGATTGGGTTACATACCTGAACAACTGTTTAAACAGCTATCCTGATTTTGACATCATCAAGTTCATTAAGAAGTATGGCCCTGAGTGTGAAAAGAGCTTTCTAAGCTGACAGAGCAAAGCTAAGAGTGATGTTTACAGTGAACTGACTAACAAGATTAAAAAACAACAGTTCTCAGAACAGTTAATTTACCAGCTAGTCCAACTTGATGCTTTACGAACTAACTATTTAATTGGTTCGTTGTTTTCAGATAACAAAACCCAGCGCAAACTCCTGAAGCGTTCTTGAAAAAATGCTAAGAAAGAAGGTTATACAAAACAAGAATGGTTGATGATCTTAGTTGGTTTACCCTTTGAAAAAGGTGCTTATCATAAGCAGTTATATGACCATTCACGTCAGGAGATCTTAGATCTTACTGAAGTTATTAAAAAGCTTTATCTAAAAACAGAGACCAACAACGATAAGCTTGAGTTTGCTGCAACTACTAGTAAAACAACAGCGCAGCTAACTAAAACTATGCCCTTAAACAGTAGTGATCTTGATAAGGATCTAATGGAGTTTAGTGGTGAGAAGTGAGGTGATAATTAGTGCCTAAGATTGAAGTTAAAAATGATGATTTAGAGCTAGCTTTAAAAAAGTTTAAAAGGATATCACTGGAAGTACGCAGGTTAGCACAACGCCATGAATACCACTTGCGCAAAGGGATGCGGTTAAGAGAAAAACAAAAGATAGCACAGAAAAAGCGCAGGAAGTTTCGCAGTTTAGCTAGCCATTAAGATGGATAATAAAAACCCCCAGAAACTTATTACTAGTGAATTGTTGGCAAACCACCGCTTTAATTTTGCTAAAGATGATAAAGGTGGGTATGATGCTAATGAAGTTGATGCATTCTTAGATCAACTAACCAAGACTTTAATCCACTATGAGGAGATGAAAAACAACGAACAAGAATTGAAAAATGCTTATGACAAGTTGTTTTCAGATCGTGATCAGATTTTAAGTCGTTGTGCTAAATTAGAAGCTGATTTAAACACCTTTTATGAAAATGGTTATGCAAACAAGGTGTTAATTAACCGGGTTCAGGAGTTGGAGGATAAACTTGAAAAACTACCTGATCGTTACACTGAAAAACTAGAAAGGATTGAAAAACTGTTAAAAAAGGTCATTAAACACTGAACTGATGGGGAGGACATTAGTAACTTTGAAGATGAGTTTTTTTAAAGATGGTTGTTGGGATAGGGATTGATGTTGTGCAATTAAAGCGCTTCTTAACTTTAGTTGAAACTAGTGATTGTTTTGCTAAACGATTGTTAACTAGCAATGAACTAAACAGTTATTGAAAGCTAAACAATAACCAAAGAGCTAATTTTCTAGCAGTGCATTGAACTTTAAAAGAAGCGATTTATAAAGCTACCAGTCACATCAAACCACTTTTCACTAAACTTGAAATTTATAAACTTAACAACCAGTACCGGTGTGAATTTATCCAAAACATCAACCTGTTGTTATCAGTTAGTTACACTAATTGCCATGTTAGTGCTATCTGTTTAGCACAACAAAATGGATAAACTATTTAAAACAAGTTTTAGATTCATAATAAGGTTTTTACAAATCCTGAGTTTACCAGTTGTTTTTCCTTACTTTTTATTAAGCTTTTTAGCTTGTTTAATTACTAGTAAAAACTATGAATCACTCCCTTATAACTATCCCCCTGAAATCCGATTCAAAAAGGTGTATAGATTGGTATCAATGTGACTTTACATTAAGGGAATTAAAGTAGTGACAGTAAATGACAAGATTATCCCTAAAAAACCAGTTTTAGTGGTAGCTAACCACAAATCTAACCTTGATCCTTTAGTATTAATTAAGGCCTTTGGCAGGTTGAAAAATAGTCCACCATTAACCTTTGTTGCTAAGATTGAACTGAAAGATACAGTCCTTTTTAAACTGATGAAATTAATTGATTGTGTTTTTATTGATCGAAAAAACATCAGACAAATTGCCAATGCATTGGAAACCCAACAACAACTAATTCGCCAGGGCACTGCTATTGCTGTTTTTGCTGAAGGGACTAGGATTTTAAGTAATGACATTGGGGAATTTAAACCAGGAGCACTAAAGGTTGCTTACAATGCTTTTGTACCTATCTTACCAGTTAGTATTGTGGGTAGCTTAGGAAAGATGGAATCAAACAAAAGGCTAAAAGAACATGGTGTTAAGAAAAGTTCAAACTATGAGGTTAAAGTAATCTTTAACAAGCTAATTAACCCAATTAGTTTTAACCAGATTGATTCTAATAACCTTGCTAATAACATTAGAAGCATTATTAGTGATGCATACACTAGTGAAAAACCAAGCAATGATTAGTTTAATTTTTGATAAAAGCAACAGTTTTAACCCCCAACTTTGTGTTGAACTTCTCAACCACTTTAAAGTGGTTATGCTAACTAAAACCATCGTTGTTGACTATCCCAGCTTTCAAGCATGAAAAGCACAACTCAAACCGTTTAAATTAGCAGTTTTTAGTGATAATTTGCAAACCGAATTAACCCCAAATTCAAAACTCACAGTTTTTAACAACTACCAGCAACTTTTAGTTGATAATAATGACCTAATTATCTTTGCAACCCCCACTTTAGTTCAGCTTTTTGATAATGAGATTGACCAGTTAATAGTTATCAATCCCACTAGTAAATCTAAAGATCAGTTTAACTGTAATTGAAACGACTTTGTTTTAATTAAACAAACTAATTTCAAGAACCATCAAGTTGGTTATTTTGATAAAAAAAGTAGTTATGAACCACCTTTCACTGTTGTTACAGACCACTTCTTTGGGAACCTAACTGATTTATTTAGTCTTTTAGTTGATAAAAAGGTAAGTGTAAATGATGTGGATATTGGCTTGATTTCCTTACAATATCTCAATATCATTGCAAATTACACCAATAAAAAAGCCATTGAAAAGATCACTGATTATCTGGTGATTACAAGCAAAATTCTCGCTAAAAAAGCAGATAATCTGCTCAATGATCATCAGGAAGAGAGTGTGTTGGAATATGATTTAGCTACTAATAACTTTCGTGATAAGATGATCGCTAACTTAGTGGAACACAAACGCTATTGTGATTCACTGGGTGAATTTGAAAAACTCAGGGTTAACCGCTTAGCTTACTTTTCCAAAGCTAATGAGATGGAGCAATTTATCAAAACAGCTAATGATCAACTTGTTACAGTTGAAGATCAACTCCCTAACTATATTAGTGTCTTAAAACTCTTTCATGCTATGAACAAGTTACTGGAGATGAGACTCAGCTCTCTTCTAACAAATAAAAACATCACCATTAAAGAGTTATCAGTTGAACAGGTTCAAAAGGAGTTGGTTTTAGCAATCAAACAGTTTAACTACCAAACAGTTTCTTTAAAGCGGGTGTTATTAAAACTTAACCATCCTATCTCTTTAATGTATTTTGTTACTGCTTTTGTGGCACTTTTAGTGCTATTAAACAACCAGGTAATAGGTTTGGAACAAAAAGATTATCACAGTGAACTTTACATCTTTTTACTAGATGAAAACCAACTGAAAACCTTCCAAGAATCACCAGATGAAATGGTAAAAAGAATCCAAGCTCAACAACAGCAAAACGAACTGATAATTGCTAAAAACAAGCAACTAAGAGCTATCAAAAACAAACAAAAGCGAGCTGATTATCTAAAAAAGAAATATGGTGAAAATTACTTAGATAAAACTAACTTAAAAGATGAAAACAACAATTAATATTGCCACCCCTACCCTAAAAAAACCTAGCAAAGAAGCTAACTTGGTTGCTAGTATCTATGGGTTGTTATTTGTTTGTGGCGCGAAAGGGATCACTTTAAGAGAACTAATTAGGATCTTTAAAAAAGCAGGGATTGAAAAGGTGAAATTAGCACTCTTAGCACTTGAAAGGAAGTTAGCAGATGATGAGCAATCAGGAGTGGAGTTGAAAAAATTTGGTAATAGTTTTTCTTTGGTAACAAAACCAATTATCAAAGACTATCTCCACTTATTATTGGCTCATAAAGTCAAAAATCCCCTTAATTCCAAAGCAATGGAAGTGTTGGCTATCATTGCTTACAACCAACCTTGCACCAGACCCAGAATTAATGAAATTAGGGGAGTTGATTCTTTTCAAATTGTTGATGATCTAATAGCAAAAGAGTTAATTGTGGAGTTAGGGAGAACTGATAAACCAGGTCGACCTTTTATTTATGAAGTGTCAGCTAAGTTCTATGATTTATTTGGCATTGATAGCTTAGATCAACTCCCTAAGATTGAGCATTTTGATCTTGATAAATTTAAGCAAGGTAGCTTTTTTGATTCCAACCGCTATGGTGATGAATAACCTTATATAATTTACAACATGGATAAAATAGCTATTTTAACTTCGGGTGGTGATGCTAGTGGGATGAATGCCACCATCGCTTATCTAACCAAATATGCAATTGCAAAGCAATTGGAAGTTTTTTATGTAAAAAACGGTTATTATGGCTTGTATCACAACCATTTTATCACCAGTAAGGAACTTGATTTAACTGACTTTTTCTTTATGGGGGGAACAGTAATAGGATCAAGTCGTTTCAAACAGTTTCAAGATCCTAGCTTACGAAAACAAGCAGTTTTAAACCTCAAAAAACGTGGTATTAACAACCTTGTTGTTATTGGTGGGGATGGGAGTTATATGGGTGCTAAAGCACTCAGTGAATTAGGATTAAACTGCTTTTGTTTACCTGGTACGATCGACAATGATGTCAATTCCAGTGAATTTACCATTGGTTTTTGAACTGCTTTAGAAGCAATTCGGGTTAATGTTGAAGCAATTTATCACACCACCAAATCCCATAACCGCTTAGCAATCATAGAAGTGATGGGGCGTGATTGTAGTGATCTGACCATCTTTGGGGGGTTAGCTACTAATGCTAGTTTTGTTGTTACTAGCAAAAATAGCTTGGATCTCAATGGCTTTGAAAAAGCAGTGAGAAAGGTGTTGCAATTCCAGAACTATTGTGTTGTTTTGGTTAGTGAAAACATCTATGGTAAGAACGGTTTACCTAGTTTAGAAATGGTTAAAGAGCACTTTGAAAACAACGCAATTAAGTGTAACCTAGTTTCACTAGGACACACCCAAAGGGGCTTTAGTCCTAATAGTATCGAACTCTTTCAGATTAGTTTAATGGCTAAACACACGATTGATCTGGTTGTAAATAATGCCAACAGTCAAGTAATAGGGATGAAAAACAACCAAGCAGTTAACTATGATTTTAACACTGCTTTTAATTTACCAAAAGCTGATAGAACCAAGTTACTTAACCAAGTTAACACTGCAATTATTTAACGATGATTGACCATTTAAAAAGAACAAAGATAATCGCTACCTGTGGCCCAGCTTTAACAAAAAGCTTGGTTAGCTTAAAGATGCTTGATGATAATGAGTATGCAGCTATTAAAAAGGTTGCTTATGCCAACATTGAAGCAATTATTAAAAGTGGGGTTAGTGTGATTAGGCTTAACTTCTCTCATGGTACCCATGAAGAACAACAAGTGAGGATCAAGATAGTAAGGGATGTAGCGAAAGCAATGAACATCCCTGTTTCTATTATGTTAGATACAAATGGTCCTGAGATCAGGATAGTAGAAACTAAAAAAGAGGGTTTGAAAATCACCAAAGATAGTGAAGTGATTATCAACACCATGAGTAAAATGATCGCTAGTGACAACCAGTTTGCTGTCAGTGATGCTAGTGGCAAATACAACATGGTTAATGATGTGAATATAGGTCAGAAAATCCTTGTTGATGATGGTAAGTTAACCCTGGTTGTCACAAGGGTTGACAAACAACATAACCAGGTTATCTGTGTTGCAAAAAACGACCACACAGTTTTCACTAAAAAAAGACTTAACCTACCCAACGCACAGTACTCTATCCCTTTTCTCAGTGAAAAGGATCTGAAGGATATTGACTTTGGTTTAAGCCAAGGTATTGACTATATTGCTGCCTCTTTTGTTAATACTGTTGCAGATATTAAACAACTGAGAGATTATCTGAAATTAAAGAATGCTAGTGGGGTGAAGATCATCGCTAAGATTGAATCTAATCATGCTTTAAATAACATTGATAAGATCATTAAAGCTAGCGATGGGATTATGGTTGCTAGGGGTGATTTGGGCCTTGAAATCCCTTATTACCAAGTCCCTTACTGACAAAGGTACATGATTAAAGCTTGTCGCTTTTTTAACAAGCGTTCTATTACTGCAACCCAAATGCTTGATTCACTAGAAAAAAACATCCAACCAACCCGAGCTGAAGTGACTGATGTTTACTTTGCAGTTGATCGGGGTAATGATGCAACTATGTTAAGTGGGGAAACTGCTAGTGGGCTTTACCCTTTAAATGCAGTAGCGGTGATGCAAAAGATTGATAAACAATCAGAAACCTTCTTTGATTACCAGTATAACGTTAACTATTATTTGAAAAACTCCACGGCAAATAAAAGTAGGTTTTGACACAACGTTGTTTTACCTTTAACAAAAAAGACTGTTCCTAAAAGAAAACTTGTTAACAGTGCCTTTAAGTATGACTTTATTGTCTATCCTACTAATAACATTAACAGGATCTATGCATTATCAAACGCACGCTTAGCAGCAGCAGTTATTATTTTAACCAACAACAAACGGGTTTACACTGGCCATGGTGTTGATTATGGGATCTTCTGTTATTTAATTGATAAAAACCCCAACCAGCTAACCAAAGCTGAACTGATTGAACTTGCTTGAAAAGCAATTAACCACTATCAGGCTTATGGTGATTTAGAAAAACTCAAACAGTGTTTAGCTGTCTATAATGAAACAATTATCAATCTTTAGATAAAAATTGGTGAGAGTTTGTAAAATATTGTACACGGTGCCTTAGCCAAGTGGACTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCATGTACATGAAAGTTAACTACTCTCCATGTGCAAACGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCAAAAGGCTGGATACCTCAGTTGGTTAGAGGGCCCGGTTCATACCCGGGTTGTCGTGAGTTCGAGTCTCACTCCAGCCACCAAAGTTACTTAAATATTAAAGGATCTATAGCTCAATTGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCAAGTCCTGTTAGATCCACCACTTTGCTGTGTTAGAATCATATTTGCCGCAATTTTGTGGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTTTATAAGCACGCGAGGGTTCGAATCCCTCAGTCTCCGCCAAATAATATTTAATCGCGGGATAGAGCAGTTGGTCAGCTCGTCAGGCTCATAATCTGAAGGTCGAGGGTTCAAATCCCTCTCCCGCAACCATGGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCATTGGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCATTAAAAGTTTTTAAAAAAGCCCTAAAAAGGGGCTTTTTTAGTGTTACCACCAATTAAAATATTTTTATAGCAAGCAGTTGCATATTTTTAATATAAGTTAGAATTATTGGTATAGTGTCTTCAGCTGTTTAATTCATATTAAAGCGCATGGAAAAAAATAGATCAGCTTTTCAACAAAACCAACAAGCATCAAACCAACCTTTTAACCAAGATCAAAACCAGTATTACCAAGATCCTAACCAACAACAATTTAACCAATCTGGTTTTGATCCAAATCAACAGCAATTTAATCAACCAGGATTTGATCCTAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAAGCTGGTTTTGATCAAAACCAACAGTATTACCAAGATCCCAATCAACAGCAATTTAATCAACCAGGATTTGATCCTAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAAGCTGGTTTTGATCAAAACCAGTATTACCAAGATCCTAACCAACAACAATTTAACCAATCTGGTTTTGATCAAAACCAGTACTATCAAGATCCTAATCAGCAACAATTTAACCAGCCTAGTTTTGATCTAAATAACCAACAATTTAACCAACCTGGATTCAACCAATCCCCAGCATTTGAAATCACACCTCAAGAGCAAAAAGCTGAACAGGAAATGTTTGGTGAAGAACCACCTCAAGTAGTTAGAGAGATCCATGAACTACCATTTGAAAAGATCCGTTCTTTTTTACAAAGTGATTTTGATAGCTATAACTTTCGCTTAAACTCACTTAAAAGCAAACTGGATAATGCCTTGTATTCACTTGATAAAACAATCCAAAACACCAATGAAAACACTGCTAATCTAGAAGCAATTAGACATAACTTAGAACAAAAGATTCAAAACCAAAGCAAGCAATTAAGAACTAACTTTGATACCCAAAAGCTTGATGATAAGATCAATGAATTGGAGATCAGAATGCAAAAACTAACCAGGAATTTTGAATCTCTAAGTGAACTTTCAAAGCACAACTCTTATCCTAATTACTATGAAAAATTGTTACCAAATGGTGGTGATAGTATGACCAATGTCTTTGAAAAAGCACTAATGATGAATTTATTGAGAACTACATTACCCCCTCAACCCCAAGTTCAATACTACCCTCAACCCTATCCATACATAAGACCTTACTATGATGAACCTATTTACGCTGGGTTTAGAAGAAGGGGTTACCGTGATGACTTCTATGAATAAAAAGCGTGTTTTAACTAATGAAACCATTTGATAAAAAACCTTCGCTGCAACCAATTTATGACATTGGTTTTGATGATGGTTATCTCCAAAGTGAGTATGAAAAAAATCGTTCTAAAACCGATGTTGATAAGATCGAAAACCAGCTTTTAAAAGAGATTAAAAGCCTGGAAGATGAACTTAAAAACCTTAAGGGCTTGAAGAATCAAGCAGAAGATAATCCTGAACTTGATAAAAAGATTAACCACTTGGAAGTTGATCTAAACCGTTTGGTTAATGAATATAAAAACTTCCAGTTCCAAAAGAACCACATGGTTGATAAGGTTAGTGAACTTGATAACTTAACCCGTTTTTATAAGAATGAACTAACCCGCTTACAACAAGAAAACGCTGATTTTCTCAACTCCAAGTATGCTAATTTAGCTAACTTCCAAGCTAACTACCACAATAAACTAAATGATTTTCACCGCTTAATAGAAAATCAAAACCAAACCATTAACCGCTTAAACCAAAAGATTAATGGTAACCAAAATCTGATTGATAATAACGTTGCTTTACTGCAAAACCCCAACATCACAGTTGAAAAAAAGAACTACTTACTAAATGTTATTGATCAACTTTACAATGAGCTTGATCAACTTGAGAATCAAAAAAGATTATTAAGTATTGAGTATGAAAATACCTATAGAGAGTTAGTTAGTGCAGATAATGAACTGCAAAATGTTTATGAAAACATCGATCAAAATCAGATCCAGTTTAAACACCAATACCAAACTTATAGAGATGAGTTAAGTCAACTTGAGCGCAAGATCCAGCTCACCAAACAAGAGTTAGTTGATAAAGAATCAGCACTAAGAGTAAAGATAGATGATGCTGATTTTTACATTAATGCCCGTTTAGCTGAACTTGATGATGTAGCTAAACAACTTAGCTTTCAAGATGGTATTACCAAGCAAAATGCTCAACATGTTGAGGATAAGTTAGTTGCTTTAAACAAAGAAAAAGACCGTTTAAATACCCAAAAAGAGGCCTTTTTTAACTTAAGACAATCTGCTTTAATTGATATCAATAAACTCCAGCAGGAAAATGAACTGTTTGCTAAGCACTTAGAACACCAGCAAAATGAGTTTGAACAAAAACAGTCTGATAGCCTCTTAAAGCTTGAAACTGAATATAAGGCTTTACAACACAAGATTAATGAATTTAAAAATGAAAGTGCCACTAAGAGCGAGGAACTTTTAAACCAAGAACGGGAACTATTTGAAAAACGCAGGGAAATTGACACGCTTTTAACCCAAGCATCTTTGGAATATGAACACCAACGTGAGTCAAGTCAACTACTCAAAGATAAGCAGAATGAAGTAAAACAACACTTCCAAAACTTAGAGTATGCTAAAAAGGAGCTAGACAAAGAAAGAAACCTCTTAGATCAACAAAAAAAAGTTGATAGTGAAGCAATCTTTCAACTCAAAGAAAAGGTTGCTCAAGAACGTAAGGAGCTTGAAGAACTATACCTTGTTAAAAAACAAAAACAAGATCAGAAGGAAAATGAACTGTTGTTTTTTGAAAAGCAGTTAAAACAACACCAAGCAGATTTTGAAAATGAATTGGAAGCTAAACAACAGGAGTTGTTTGAAGCTAAACATGCATTGGAACGTTCCTTTATCAAACTTGAAGATAAAGAAAAAGATCTTAACACTAAAGCACAACAGATTGCCAATGAGTTTTCCCAACTAAAAACTGATAAGTCAAAGAGTGCTGATTTTGAACTAATGTTGCAAAATGAGTATGAAAACTTGCAACAAGAAAAACAAAAGTTATTCCAAGAACGTACTTACTTTGAAAGGAATGCTGCGGTTTTATCAAACCGGTTACAACAAAAACGTGAGGAGTTATTACAACAAAAAGAAACGCTTGATCAGCTTACAAAAAGCTTTGAGCAAGAACGGTTAATCAACCAAAGGGAACACAAGGAGTTGGTTGCATCAGTTGAAAAACAAAAGGAGATATTGGGCAAAAAACTCCAAGATTTTTCCCAAACTTCACTGAACGCTTCTAAAAATTTAGCTGAACGGGAGATGGCAATCAAGTTTAAAGAAAAGGAGATAGAAGCAACTGAAAAGCAACTGTTAAATGATGTTAATAATGCTGAAGTTATCCAAGCAGACTTAGCACAACTCAACCAATCACTTAACCAAGAACGCAGTGAATTGCAAAACGCCAAACAAAGGATTGCTGATTTTCACAATGATTCACTAAAAAAACTCAATGAGTATGAACTTAGCTTACAAAAACGGTTGCAAGAATTACAAACCCTTGAGGCTAACCAAAAACAACATTCATATCAAAATCAAGCTTACTTTGAAGGTGAACTTGATAAACTTAACAGAGAAAAACAAGCTTTTTTGAACTTACGTAAGAAACAAACTATGGAGGTTGATGCTATTAAACAAAGGTTGAGTGATAAACATCAAGCTTTAAATATGCAACAAGCAGAGCTAGATAGAAAAACCCATGAGTTAAATAATGCTTTTTTAAACCATGATGCGGATCAAAAGAGTCTACAGGACCAACTAGCAACTGTTAAAGAGACCCAAAAACTAATTGATTTAGAACGTAGTGCACTGCTTGAAAAGCAACGTGAGTTTGCTGAAAATGTTGCTGGTTTTAAGCGCCATTGGTCTAATAAAACTAGTCAACTCCAAAAGATTTATGAACTGACCAAAAAACAGGAAAGTGAGCAAACCCAAAAGGAAACAGAACTAAAGATTGCTTTTAGTGATCTACAAAAAGACTATCAGGTTTTTGAACTCCAAAAGGACCAAGAATTTAGACAAATTGAAGCTAAGCAACGTGAACTTGACAAGTTAGCTGAAAAAAATAATCAGGTCAAACTAGAACTTGATAACAGGTTTCAAGCGCTGCAAAACCAAAAGCAAGACACAGTACAAGCTCAGCTAGAACTGGAACGTGAACAACACCAGTTAAACCTTGAGCAAACTGCTTTCAACCAAGCTAATGAATCACTTTTAAAACAACGTGAACAACTCACCAAAAAGATCCAAGCTTTCCACTATGAGTTGAAAAAGCGTAACCAATTCTTAGCTTTAAAAGGGAAAAGGTTGTTTGCAAAAGAGCAAGATCAACAACGCAAAGATCAGGAGATCAACTGACGCTTTAAACAGTTTGAAAAGGAATATACTGATTTTGATGAAGCTAAGAAAAGGGAACTTGAAGAGCTTGAAAAGATCAGAAGAAGTTTAAGTCAAAGCAACGTTGAATTAGAGAGAAAAAGAGAAAAACTGGCTACTGATTTCACTAATTTAAATAAGGTTCAACACAACACCCAAATTAACCGTGATCAACTTAACAGTCAGATCAGACAGTTCTTATTAGAACGCAAAAACTTCCAACGCTTTAGTAATGAAGCTAATGCTAAAAAAGCCTTTTTAATTAAGCGCTTAAGAAGCTTTGCATCCAATCTAAAACTCCAAAAAGAAGCGTTAGCAATCCAAAAACTAGAGTTTGATAAGCGTGATGAACAACAGAAAAAAGAGTTACAGCAAGCTACTTTACAACTAGAACAGTTCAAGTTTGAAAAGCAAAACTTTGACATTGAAAAACAACGCCAACTAGTTGCTATTAAAACTCAGTGTGAAAAACTTAGTGATGAAAAAAAGGCACTAAACCAAAAGCTAGTTGAACTAAAAAACTTATCCCAAACCTATCTTGCTAATAAGAATAAGGCTGAATACTCCCAGCAACAACTCCAACAGAAATACACCAATTTACTTGATCTGAAGGAAAACTTAGAGAGAACCAAAGATCAATTAGATAAAAAACATCGTTCTATCTTCGCTAGATTAACTAAGTTTGCAAATGACTTACGTTTTGAAAAAAAGCAACTGTTAAAAGCACAGCGCATAGTTGATGATAAAAACCGTCTTTTGAAAGAAAATGAACGTAACCTCCATTTCCTTTCCAATGAAACAGAACGAAAACGAGCAGTTCTCGAAGATCAAATTTCTTACTTTGAAAAACAACGTAAACAAGCTACTGATGCGATCCTAGCATCACATAAAGAAGTTAAAAAGAAGGAAGGTGAACTGCAAAAGTTACTGGTTGAATTAGAAACAAGAAAAACCAAACTCAACAATGATTTTGCAAAATTCTCAAGACAACGTGAAGAGTTTGAAAACCAACGCTTAAAGCTCTTGGAACTGCAAAAAACCCTGCAAACCCAAACTAATTCCAACAACTTTAAAACCAAAGCAATCCAAGAGATTGAAAACAGTTATAAAAGGGGGATGGAAGAACTTAACTTCCAAAAGAAGGAGTTTGATAAGAATAAATCACGCTTATATGAATACTTTAGAAAGATGCGTGATGAGATTGAAAGAAAGGAAAGTCAGGTTAAGTTAGTTTTAAAAGAGACCCAAAGGAAAGCCAACCTCTTAGAAGCACAAGCCAACAAACTTAACATTGAAAAAAACACTATTGACTTTAAAGAAAAAGAGTTAAAAGCCTTTAAAGATAAGGTTGATCAAGACATTGATTCAACCAATAAACAACGCAAGGAGTTAAATGAGCTTTTAAATGAAAACAAGTTATTACAACAATCACTAATCGAAAGAGAAAGGGCTATTAATTCCAAAGATTCACTTCTAAATAAGAAGATAGAAACGATTAAACGCCAACTCCATGATAAGGAGATGCGGGTGTTGCGCTTGGTTGATAGAATGAAATTAGCAGAACAGAAATACCAAACAGAAATCAACCGCTTAAGAACCCAAACTTTCGATTCTGAAAAACAAGACATTAAAAACTTCTTCCCACCGTTATTTAAAATTAACGGTAATGATATGGCCTTTCCTTACTTGTACCCCTGACTATATCCTCAACAAAAGCAAGATGATAATACTCTGCAAATTCGTCAGCTTTTTGAACAGCAGCTGCAGTTCATGCAACAACGCTATGAAAATGAGTTAAATGAATTGCGTAGACAACGTAATTTACTTGAGAAAAAACTTGATCAAATCCAACTAGAATCCCAACTTAATAATAAGCAAAGTGAGTTTAGTAAGGTGGAATCAATGATGGAAAAACTACTTGAAAAAACTGAGAGTAGGTTAAATGATTTTGATCAGAAAATTAACTATCTCACCAAGAAAGTTAACCAACACAACACCTATCAACCAAGTTCCTATCAACCAACTCCTTCTTATCAAGACAGTGATAAACAACAGTTGTTATTTAGAATCCAAGAACTGGAAAAACAAAACTTATTCCAACAACAATTTCAACCTGCACCAGCTGTTGTCCAACAACCTACTAGTTTTGCAGCCCCTAACATCACCAAACAACAGCAAATTGCCCAACTTAATGCTGAAATTAACAACATTAAAAGGTTGATTGCCCAAAAAGCAGCAAGTAAATAAAGATGGTTAATAATGAATATCAACAACTAAACACTTTAGTTGAGAGTGATGATGAAGCGGATCTTGTGATTGCTAACCTAGTTAAACAACTCAATGAACTAAAGCAAATCCTTGTTTCACTAGATAATCAAGAAGCAAGTGCCACTGCAGTTACTGATAAAAAGGAAGAGGAATACAACCAAAACCAATCCAGTTTCCATAACTTCAGCAAAGAAACACTGCAAAAGCAAGCAAAACGTGGTTTTCTTTTACTGGAACGCTGTTCGTTGGTTGGGTTACAACAACTAGAGTTGGAGTATGTTAATTTGTTGGGCAGAAGTTTTGATTCTTATCAACAAAAAACAGAGCTTTTAAACAACTTAAAGGAGCTTGTTGATGAACATTTCAGTGATACTGAAAAAATTATCAATACCCTTGAAAAGATCTTTGATGTTATTGGCGGTAGTGAATATACCCCTGTCTTAAACTCGTTTTTTAACAAGCTTTTAAGTGATCCTGATCCAATCCAACGGGAAATTGGCTTAAGACAATTTATCATCACTCTCCGTCAGCGCTTTAAAAAGTTATCACAAAAGATTGACAGTTCTCTCAAACAGATAGAAACAGAGGCTAAAATAGCCACTGAACAGGTTCAAAATAGTGAAGTGATGTTCGGTCCCCCTGATATTGCTAATGATCATGAGTTAAACCTGAACTGACCTGATAGTGAAACAGATGCTATCTTAAGTTCAATGGAAAATGAATTGGAAGCTGCTTTATTAGCAAAACACCAAGAAGAACCACCGTTAATTGTTACCCCACCCAGCTTAATAAAACCAACTGTTAGTCAACCTGAAGTTGAAGTTGTTACACCTACTAACAACACTAATTTCCAACCCCAAGTTGATCTCAAACCTACTGATTTGAAAAAACAACAGAAGAAAAAACCACTTAACTTTATTACCCGTCCTGTTTTCAAAAGTAATTTGCCACCGAAACTAAGTAAGGATGACATAGTTCATTATGCGCACCAGTTACTTGAAAAAAATACCCATAATGAATAGTGATAGTGATCTAAAACTCCAAAAGGTGTGGATCGAGCGGCATGTTGATCAAGATGAACTTAGTTTAACAACTACTGCAGTTGAACTTAAAAAGAGTGATGAACAAAAACCTGTTGCCATTAAAAGTAGTGACTTTATTGGTCATGAAGAGTTAATCTCTGTTCCAGTTTTACTAATCCCAACCCCTGTTGTTAAAGAGATTGATCAACCAGCAGTTATTCCTCCAGTTAAAGCAAAACCAAAAGCAACTAAAAAGAAAACTCCTGTTAAATCAAAACCAACTAGTAAATCAACTAAACAAACAAAACCTAAACAATCCAAGCCCAAATCAAAACAAGTTCAACAAACCAAAGCTAAACCAACCCAAATTCAAACAAAAAAAAGCAATAAAAAAACCAGATCTTAATCTGGTTTTTTTAGTGTTAACAACAGTAGTTTTAACTAAAAACTGGAAAGGAATAGGAAGAACTACCTGGGGTTAATCCTAGTTTAAACCGTGCGGTGTTAAAGTAATTGCTTTTAGAGCGATAACTATTATTTTTATTCTCATTATCATCATCAGAACCATTGGCATTAGCTGTGGTACTTTGAAAAATGTCATTTACTGCATGTGCAACCCCAGTAAGTGCATTAACTATCATAATAAGTCCTGCACTAAAAGTTCAGAAAGATAATCCACCAACAACTTGCTTAGCTTGTGCTTTTTCTAATTTATACATATAACTCCTTTTGTCTATTAATCACATCTAAATCTAAAAGTGCCAAAGTTTTATAATTGATCAACTGTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCATTAACTTTATTTGCCTATCAGTTAAATAACTGGTAGGCTTTTTTATTGTTTTGTAGTTTATCAAGGGTTAATTTAAGTTGTAGTCATTTCATTTTGGACAAAAAGAAATTTTTATGCTAAGATAAAAGTGTTTAAAAGTGTCGCAAAGTGTGACAAAGTGGAAAAAATGCTGCTAGGTACCTTTAATCTTACCCTTGATAACAAGAACAGAATTAGCTTGCCAGCTAAGCTCCGTAGTTTCTTTGATAGCAGCATAGTTATTAACCGCGGCTTTGAAAACTGTTTGGAAATTAGAAAACCTGCAGACTTTGAGAGTTATTTTCAAACCTTTAATAACTTCCCTAACACCCAAAAAGACACAAGAACATTAAAACGCTTAATCTTTGCTAATGCTAATCTAGTTGAACTTGATAGTGCAAACAGAATCCTAATCCCTAATAACCTAATTAGTGATGCTAAGTTAGATAAAGAGATCGTGTTAATTGGTCAATTTGACCATCTTGAAGTTTGGGATAAAGTGCAATATGAACAATATCTAGCTAGTTCAGAATCACTAGAGACAGTAGCTGAAAGGATGAAAGATGCTAAATAACCAACAGATCCACCAGAGTGTACTGATCAATGAAGTGATCCATAACCTCAATATTAACCCTTGTGGTAACTATTTAGATCTAACTGCAGGGTTTGCAGGACACAGTCAAAAGATCTTAGAAAAACTAACAACAGGAACTTTAACAATTAATGATGTTGATAAAGAAAGTATTAATTTTTGCCAAAAGCTTTTTTTTAAAAACAACAACGTTGTTATTATTCACGATAACTTTGCTAACTTCCCAGTTCATCTTAAACAACTATCAATAACCAAGTTTGATGGGATCTTAATGGACCTTGGTGTATCAAGCCATCAACTCAACCAACCTAATCGCGGTTTTAGTTTTAAGAATGATGGACCGATTGACATGCGTATGGACCAATCCAATCAGAAAAATACCGCACTAACAGTTTTAAAAAACTTAACTGAACAAAAGTTAAGTCTAATCCTTAAAAAGTATGGTGATATTAAACACCCTAAACCAATTGCTATTGGATTGAAAAAAGCAGTTCAAACTGAAAAAAATCTTACCACAACTCAACTAGCAAAAGTGGTAAAAGAATGTGCTACTGGATTTGAAAAATACCAATCAAGAAACTATCTTGCCAAAGTTTTTCAAGCAATTAGGATCTATCTTAATGATGAGATTACTAATCTGAAAACTGCGTTAACTTTTATCCCTAATCTTTTAAAAAACAACAGCAGGTTTCTTGTGATTGTTTTTCACTCCATTGAAGAAAAAATTGTAAGGAATTTCATTGCAAAACTAACCAGCTTTATCCAACCTGAAGCTCTACCCATTAAACTCACTCCTGCTTACCAGTTAATTACAAAAAAACCAATCCTACCTTCCCAAAAAGAACTTGAATTAAACCCGCGTTCGCGTAGTGCCAAACTCTTTGTTATCCAAAAAAACTAGTATGTACAAACCAAAAAATATTAACAGCGTATTAACCTTTTATAAGGATCAGATCCAACTGGTTGTTAGTGATGATCAAAACCAGTTCAACATCTTGTTTTACCAAACAATTGATAACGATGGCTTTTATTCAAAACAACAGTTGAAAAACAAACTAAGACTCAAGTTAGCATTAAACCAACTAGTTGATCAAGCTAACTATTTTCTTGGTTTTAAACTGGAAAAGGTAGTTGTTGTTCTCGCTGAACTGATTGATGATTTGAAGATCCATAATTTCAAGAGTGAGATCTTTTTTACTGGTTATGATTTTGATCATAAAGCGATGATTAAAAAAGAAAAACAACGCTTTTGTGAGCAAAATAACCAACTAACAGTTATGGATACAATGGTTTTAAACTACCATGATGTTATTAACAATAAGATCACCAAAAGCTTTGCATTTAACAAGAGCTATGTAGCTAATTTAGTGGCATATTCCTCTAAAAGTAACCTGATCGGGGAGTTGAAGTTCTTTTTAAAAAGAAACGTTAATCTTAAGGTTAAGAAAATTATTAGTCACCACTTAGCATTAGCCAACTCCTTAAGTAAGAAACAAAACAACATGTTTGTTTATTTAGGACAAAAAACTACTGAACTGATGCTATTTATGGACAATGCTTTAGTTGATGTTATTACCAACCAGTTTGGTAAAAACCACTTTATTGATATTCCAGCTAACCAGGAAAACAAACCACTGCTTGAGTTTTTAGTTGATAACACCACTAAGATTGGTGATTGTTATTCGCTTGGTATGACCTATACAGATGGTGATAGTTACAAAGAGATTAAGGCTTTGACTATTGGTGATTTAATGCAAACAGTTAGTGACAAGATCAAAACCTTAATTGATTTTATTAACAGTGGTTCTCTAACTTTTTTCAACAAGTTTAAAACCTTACCTAAGCTATTGTATTTTTATACAAGATCAAAACAAATTACCAACCTTTTTCAAGCTAATGTTGCACTTATCAATCCCCAGTTTAAAACTGTTGATATTTATAAGAACAAGATCCAGTTTATTAGTGAAAACTACCTGTTAAGCTGTGAAGCGATTAGCTTGCAGATTACCAATAGAATCAAAAACCAAATTAGTTTTGATTTCACAAATGCTGATAATATTCAAAAACCTAAACCAAAAAAACACTTCATGATCTTATCAAAACACCTAACAAAGTTTGTCCAACGCTTGGTTAAATAACTATGGATGAAAATGAAACTCAATTCAACAAGTTAAACCAAGTTAAAAACAAGCTGAAAATTGGTGTTTTTGGGATTGGAGGTGCTGGTAATAACATTGTTGATGCATCACTTTATCACTATCCTAATTTAGCAAGTGAAAACATCCACTTTTATGCTATAAATTCAGATTTACAACACCTTGCATTTAAAACGAATGTTAAAAATAAACTCTTAATTCAAGACCATACTAACAAGGGCTTTGGAGCGGGGGGTGATCCAGCTAAAGGAGCTAGTTTAGCAATAAGCTTTCAAGAACAGTTTAATACACTTACAGATGGGTATGATTTTTGTATCTTAGTTGCTGGATTTGGTAAGGGTACTGGTACAGGTGCTACCCCAGTTTTTAGCAAGATCTTAAAAACTAAGAAGATCTTAAATGTTGCTATTGTTACCTATCCATCTTTAAACGAGGGATTAACAGTGAGAAACAAAGCCACTAAGGGGCTTGAAATTCTCAACAAAGCAACTGATAGTTACATGCTATTTTGTAATGAAAAATGTACAAATGGTATCTACCAACTAGCAAACACAGAGATAGTCAGTGCCATTAAAAACCTAATAGAACTAATTACTATTCCTTTGCAGCAAAACATTGATTTTGAAGATGTACGTGCCTTTTTTCAAACCAAAAAAACTAACCAAGATCAACAGCTTTTTACTGTTACTCACCCCTTTAGTTTTAGCTTTGATAGTAAAGATAGTATAGAACAGTTTGCTAAACAGTTTAAGAACTTTGAAAAAGTTAGTTATTTTGACCACTCTATAGTAGGAGCTAAAAAAGTGTTATTGAAAGCTAACATTAACCAAAAGATAGTCAAGCTTAACTTCAAGCAGATCCAAGATATTATCTGAACTAAAATTGACAACTACCAACTTGAGATTAGGTTAGGGGTTGATTTTGTGACAACCATCCCTAATATCCAAATTTTTATCCTCAGTGAACACAAAAATCCAGTTTCGCTTCCCATTGATAATAAATCAACTGAAAACAACCAAAATAAGTTGAAACTTTTAGATGAGCTGAAAGAACTTGGCATGAAATATGTTAAGCACCAAAACCAAATCTACTAATTAATTTAATTTATCGTTTAGAATTGCTATCTTAAGCAATAGTTTATGGGTCAAATCAATCGGAAGTTTAGCGAAAAGCAGTTCTTACTTTTTGTTGTTAACTATATTGCTGGATTTGGCTTTATTGCTACTGCTATCTCACTGTTTCGCTTAGGACCTTTTTCTTGGTTAATCTTTCTGCTTGTTAGCTTAGTTAGTTTAATTGTTACCTTATCATTCGCACGGCTTTCATCAATAGATAGTCAAAACTATGGTGGGCCTTATCTTTGGGCTAAGAAAGCGGTTGATAAAGAGAAGATAGCAGGGAGAATGTTTAGCTTTTTTACGGGGTGAAATAACTTTATCATTGGTCCTCTTTCAGCAGCAACTGCACCACTTTTTATCCTCAATTCCTTTAGTGGTATTGATGGGATTAGAGGTAACTTAGTTAACACTTGAATCCTAATTGCAATAGGTTTTTCTTTTTATGTATTACTAGCATTTATCTCAACCAAAGGAACCTCACTAAACAAGAAACTAATAGCACTATTTGCTTCAGTAAAGTGGATTGTGATCCTCTCAGCACTAATAGTAGCAATCTATGTTATTGCTAGAGATGGTAATGGTTATAGTCAAAACAATAACTTAGAAAGTGGTTTTTTTGGGAGAAGAGAGATTAGTTTTGCACAGATAGCAACGGTATTTATTACCTTCTTTTATTCTTATGCAGGGGTTGAAGATATCTCAGTGATGACTCCTGATGTTAAAACTAATAACTTTAGAAAGATATTAATTGTCTCTTTTATAGCAGTTTTCCTCTTTTATTTCATTGGGATTATTATTCTAAATGGTTTGCAAAACATTGCTCAAAGAGGTGGGGAAGCCAATTCAATTGGTAATGTAGCGGATATCTTTAAAAAAGCTGCTGGGCTTGGGACTTTAATCTTTTATGGAGTTGGAGCATTGTTTAACAATGTCTCAACCAGACTTTCAACTATTATTGCCAACTCCAGAAAGATTCTTCCGCTTGCTTATGATAACTATTTACCTAGTTTCTTTTACAAGCAAAACAAAAAAGGTGAGTTTCAGAATGCAATTTGGTTTACCTTTGGTACTACTTTAATTGCAATGACTTTGCTTGTCTTTATCCCTTTAGTTGCTTCTAACTTTGATTTTGATAATGCTACTGAGTATGCAGCATCTGTTGGCTCAGCTGCAACTTTGCTACAATATATTTTTGTTTTTTTTATAATCTTTAAGTTTATCTATAAAAAAGAACCACTCTACCAGAAAAAATGGGTTAAAACAACTGAAGAATTATTGTTTTGTTTAGGAACAATTGTCATTGTTTTAATGTTGTTGGTTTATCTGTTTCCTGTTATAGATGGATTTTCAAAATGGGAAACTAAACACACATTAACAATTGTGTTATATGGGGTTTTAAGCCTGATAGGATTGGTACTTTTTTTGCTCCAAGAATACAAACATAAAAACAAGCAAAATGCAAACAAACAAACAACCCAAACAACAGTTTAGTGAAAAGCAATTCATTGCTTTTGTCTTTAACTATATTGCTGGATTTGGCTTTATATCAGTGGTGATGACCATGTTTGATGTTGGGCCATTTTCCTATCTTGTTTTAGGGTTAACTTCGTTTGCTATTTTAGGAGTTGTGCTTTCTTTTTCTCGCCTTTCAGTTCTCTGTGGTAATAGTGCTTATGGAGGGAGTTATTTAATTGCTAAAAAAGCAGTTGGTACTAACAGTAAAACAAAAAGGTTTTTTGTTTTTTTAAGTGGGTGGAATGTATCGTTAACAGGATCTTTTAACGGTGTTGTTATTCCAGCAGTATTAATCTTTTCCTTTGCAGATATTCCAGTAGTTAAAGCGAATAATAACATCATTATTGGCCTTTTAGTAGGTGGGTTTTTGTTGTTTGGCTTACTTACTTTTATCTCGTTATTTGGTTTAAAAATTAACAAGAAAGCAATCTTTTATTTTGCTGTTATTAAGTGGATAGTAGTAATAGGTGGGTTTATCTTAGGGATCTATTTAATTGGTACTACCAATGGTAAAGGTTTTGTTGAAAACAATTTAATTGGGACTAGGGAAAACATTGATTTTTTCAAGATTATCTTTATTAGTCTGGCTTTAACCATTGCTTTTGCAGGGACAGAGGATTTAGCTTCGATTACTCCTGATGTCAAGTCAAATAACTTAAGAAAGTGTTTTTTAATTGCCTTTGGGTGTGTTGTGTTACTTTACCTAGTTGGGTTTGTTATTATCAGTGGACTTGATGGGATTAGAGGTTATGGATTAGCATTAGGTAATAAAGATCCCAAGGCAATTAATAACTATGGATCTATCTACCGTTTGGTAGGAGGAGTTCCTTTACTTGTTATCTATGGACTTGGGTTACTTGTCAATTCCTTAGCATCACGCCTATCAATGACAATTACAACAGCTAGAAAATATGTAGCTTTAGCTCAAGATGGGTTTTTACCCTCTTTTTTAGCAAAAACTAATAAACATAATGAGTATCATCATGCAGTTTTAATTAGTAATCTAATGACTTTATTAGTGATGCTAATTATGGTAATAATCCCCTTTTTACCAGACCATAACAACAATAACAATAGTTTGTTTAATGCTATTGAACAGTTGGTTACAGTTACCATTGAAATGGCTGCAGCCATTTCTTTGATCCAATACTTTATTACCTTTATCTTCTTTTTTATGATCTTTGCTAAAAAGGAAAACCAGAAGTTAATTCCCTTGTGAGAAAAGGTTAGTTATGTAATTAGCTTTGCTTTGGTAAGTGTGTTGTTGTTTGTACCACTTTTCCCTTTTAATCAGTGAACAGTGTTTAACACCTTTAAGATAGTTGTTCTAATTTGTTTTTATCTACTTGGTGTTGGTTTTTTTGGTTATGCTGAATGGAAAAATAAAAACAAATACCAATTAATGAATAACAATAGCTAATCTACAGTTCATTAAGCGAAATTGCTTTTGAATCGGCAACAACAAACCCTTCTCCAACTCCCCCTCCCCTTCAACTTCCGCTTCCTCTTCTACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACATTTAAATAAAGAAAACACCCGGTGGGTGTTTATACCTAACTTTTCACCTGACATCTGAACAGGAGCAGGGTATCGCAAACAAGGTAACAATAATGGCATCTCCTTGACCAGGTGAAACCTAGTAGTAGTAGCAACACGTTTAATCCCAATTCTTCTGATAATAAAGTCACTCAAGGTGGTGGCTCCCCAGCCAAAAAAACAACCACCTATCCTGCTTTACCAAACTCCATCAGTCCCACCAGTGACTGTTAAAATGTTTATTAAAAAAACAAAATAAAAAGCGGTTTTATACAATGTATAACCTGTCTAAAAGACAATTTCATGAAACAGTATTTAGATTTAGCTAGTTATGTTTTAGCAAATGGTAAAAAAAGAAAAAACCGTACAGATACAGATACTTTAAGTGTCTTTGGTTACCAGATGAAATTTGACCTTACTAATAGTTTTCCTTTATTGACAACTAAAAAGGTTAATTGGAAGGCAATTGTCCATGAATTGTTGTGATTTATTAAGGGTGATACCAACATTAAGTACTTAGTTGATAATGGGGTGAACATCTGAAATGAATGACCATATGAAAACTTTAAAAAATCACCAAGTTTTCAAAACGAAACACTCCAAGAATTTATCTTAAAGGTTAAAACTGATAATGAGTTTGCTAAACAATTTGCTGATTTGGGTCCTGTTTATGGCAAGCAATGACGTAATTTTAATGGTGTTGATCAACTCAAAAAAGTCATCCAAGAGATTAAAGAAAATCCCAACTCAAGAAGGCTAATTGTCTCAAGCTGAAACCCTAGTGAATTGGAAAAAATGGCATTGGCTCCTTGTCATTCACTCTTTCAGTTCTATGTTGAAGAAGATAAACTAAGCTTACAGCTTTACCAGCGCAGCGGTGATATCTTTCTTGGTGTCCCATTTAACATTGCATCTTACGCCTTACTTGTGTATTTAGTTGCTCATGAAACTAAGTTAAAACCTGGTTATTTTATCCATACACTAGGAGATGCACATATCTATGAAAACCACATTGAACAAATTAAATTACAACTAACAAGAACAACCCTAGACCCCCCTCAAGTGGTTTTGAAAAGTGATAAATCAATCTTTGCTTATAGTTTTGATGATATTGAGTTAGTTGGTTATAATTACCATCCATTTATCTATGGGAGGGTTGCAGTTTAATGCTAATTGCTATCTGAGCGATGACACAAGAAGGACTAATAGGTAATAACAACACTTTACCTTGGATGATTAAACAAGAGCTAGCTCACTTTAAAAAAACTACGTTATTTCAAGCTTTGTTAATGGGGAGAAAAACTTACGAATCACTCCCCAAGGTATTTGAAAAAAGAACAATATTTCTCCTTTCAAAAGATCAAAACTACCGTTTTGAAGAAAAGGGAAGTGAAGTGAAAGTTATTAATGATTTTTGACCACTAATTAAAAGTTACCAAGCAAATAAAGAAAAGGATTTGTTTATTTGTGGTGGAAAAAGTGTGTATGAACAGACCATTAATGAATGTGATCAGTTAATTGTTTCAATCATTAAAAAGAAGTATAAGGGTGATCAGTTTTTGAAGGTTGATCTCAGTAAATTTGTACTTAATGAAGTTGTAGAGTTTGAGGAATTTAATGTTAATTATTATAGAAAGAAACAACAATAAGAGATATGGCAGCTAACAATAAAAAGTACTTTTTAGAATCATTTTCCCCACTTGGGTATGTAAAGAATAATTTTCAGGGCAACTTACGTTCTGTAAACTGGAATTTGGTTGATGATGAGAAGGATTTGGAAGTGTGAAACAGGATTGTTCAGAACTTTTGGTTACCTGAAAAGATCCCTGTATCCAATGACATCCCCTCATGAAAGAAACTCTCAAAGGATTGACAGGATCTGATCACTAAGACCTTTACTGGTTTAACACTACTTGATACTATCCAAGCTACCATTGGTGACATCTGTCAAATTGATCATGCTCTAACTGATCATGAGCAGGTTATTTATGCAAACTTTGCTTTTATGGTAGGGGTACATGCCCGTTCCTATGGAACGATCTTCTCAACTTTATGTACATCAGAACAGATTAACGCTGCTCATGAGTGGGTTGTAAACACTGAAAGTCTCCAGAAAAGAGCAAAGGCATTAATCCCTTACTATACGGGCAATGACCCGTTAAAATCAAAGGTAGCAGCAGCTTTAATGCCTGGGTTTTTACTGTATGGTGGGTTTTATTTGCCTTTTTACTTGTCATCAAGAAAACAACTACCAAATACATCTGATATTATCCGCTTAATCCTTCGTGATAAAGTGATCCATAACTATTACAGTGGTTATAAATACCAACGTAAACTAGAAAAACTCCCTTTAGCAAAACAAAAGGAGATGAAAGCATTTGTTTTTGAACTAATGTATCGGTTAATTGAACTTGAAAAGGACTATTTAAAAGAGCTTTATGAAGGGTTTGGAATTGTTGATGATGCCATTAAGTTCAGTGTTTACAATGCTGGTAAGTTTTTACAGAACTTAGGTTATGACTCCCCGTTTACTGCAGCAGAAACCAGGATTAAACCAGAGATTTTTGCCCAACTATCAGCACGTGCTGATGAAAACCATGACTTTTTCTCAGGAAACGGTTCGTCGTATGTGATGGGAGTTAGTGAAGAGACAAATGATGATGATTGGAACTTTTAAGTTATGCATAAAGATATCAAACTAGTTAAGGAAACTGAAATTAGAAAACCAATTGGTTCTCCTTTTATTGTCTATTTTTCATCTATCTCCAACAACACCCACCGTTTTATTGAAAAACTGGGTTTTCAACACAAAAGAATCCCAGTTGATATAACCCAAAGCATTACTGTAAGTAATGAGTATGTTTTAATCTGTCCAACTTATAGTGGTGGGGGTAACCAGGTTGAAGGAGCGGTACCCAAGCAAGTTATCCAGTTTTTAAATAACAAGCATAACAGGGAGTTATGCAGAGGAGTTATTGCATCTGGTAACACTAATTTTGGAGATACTTTTTGTCTTGCAGGAACTGTTATTTCCAAAAAACTAAACGTCCCTTTGTTGTATCAGTTTGAACTTTTGGGAACAAAAAATGATGTAGAACAAACCCAAAAAATAATTGCCAATTTCTTTCAAAACAGCAACTAGTATTTATAGTTATCCACTATGACATCCAAAGAAAAAATCCCTACTTTTAATACTGAAGAAGATGTTGAAAGTTACATTTCTTTTAATGCCCAAGCCAAAATCTATGATGATTTTGCAATCGATTTACAAGCAGTTGAAAGCTATATTCAAGAGCATGTAAAACCCAAAACTAAGGTCTTTCATTCCACCAAAGAACGCCTTGATTTTCTGATTAAGAACGATTATTATGATGAGAAGATCATCAACATGTACAGTTTTGAACAGTTTGAAGAGATCACCCATAAAGCATATTCATACCGCTTTCGTTATGCTAACTTCATGGGAGCATTTAAGTTCTATAATGCCTATGCTTTAAAGACATTTGATGGTAAGTACTACTTGGAAAACTATGAGGATAGGGTGGTGATGAATGTATTGATGTTAGCTAATGGTAACTTCAATAAGGCATTAAAACTCTTAAAACAGATTATCCTTAACCGTTTTCAACCAGCAACCCCTACCTTTCTTAATGCTGGTAGAAAGAAACGTGGTGAATTTGTTTCATGTTACCTGTTAAGGATTGAAGATAACATGGAATCAATAGGTAGAGCGATAACAACTACACTACAACTATCAAAACGTGATGGGGGAGTAGCACTTTTGCTTTCCAACTTACGTGAAGCGGGAGCGCCCATCAAAAAGATAGAAAACCAATCATCAGGGATTATCCCAATTATGAAATTGTTAGAGGACTCTTTTTCCTATTCCAACCAACTTGGACAAAGACAAGGAGCGGGAGCGGTGTATCTCCATTGTCACCATCCTGATGTTATGCAGTTTTTAGATACTAAAAGGGAAAATGCTGATGAGAAGATCAGAATTAAATCACTCTCCTTAGGACTTGTGATTCCAGATATCACCTTCCAATTAGCAAAAAATAACGAGATGATGGCACTTTTCAGTCCATATGATATCTATCAGGAGTATGGTAAGGCTTTATCTGATATCTCAGTAACTGAGATGTATTATGAATTGCTTGAAAACCAACGCATTAAAAAGACCTTTATTAGTGCTAGAAAGTTCTTTCAAACAATTGCTGAACTCCACTTTGAAAGTGGTTATCCCTACATCTTGTTTGATGATACAGTTAACAGGAGAAATGCCCACAAAAACAGGATAGTAATGTCTAACCTTTGCAGTGAAATTGTCCAACCATCTTTACCTTCTGAATTCTATTCAGACCTTACTTTTAAAAAGGTAGGTAGTGATATTAGCTGTAACTTGGGGAGTTTAAATATTGCTAGAGCAATGGAAAGTGGTAGTGAGTTAGCTGAATTGATTCAACTAGCAATTGAATCACTGGATTTAGTGTCAAGGATCAGTAGTTTAGAAACCGCTCCTTCCATTAAAAAAGGTAATTCAGAAAACCATGCGTTGGGATTAGGAGCGATGAACTTACATGGATTTTTAGCAACAAATGCTATCTATTATGATTCAAAGGAAGCGGTTGATTTTACTAACATCTTTTTTTATACAGTAGCATACCATGCGTTTAGTGCTTCCAATAAATTAGCATTGGAACTAGGTAAATTTAAAGACTTTGAAAATACTAAATTTGCTGATGGTAGTTACTTTGATAAGTACACTAAGGTAGCTAGTGACTTTTGAACATGTAAAACAGAAAAAGTTCAAGCCCTTTTTGATAAATACCAAGTAAAAATTCCAACTCAGGAAAATTGGAAGCAATTGGTAGCAAGTATCCAAAAAGATGGACTTGCAAACTCCCATTTAATGGCTATTGCCCCAACTGGATCTATCTCATATCTCTCTTCATGTACCCCTTCACTTCAACCAGTAGTATCTCCTGTTGAAGTGAGAAAAGAAGGGAAGTTAGGACGGATTTATGTCCCTGCTTATAAGCTTGATAATGATAACTATCAGTACTTTAAAGATGGTGCTTATGAACTGGGCTTTGAACCTATTATTAACATAGTAGCAGCAGCCCAACAACATGTTGATCAAGCAATCTCTTTAACCTTGTTTATGACTGATAAAGCTACCACCAGAGATCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTAGTTCTATCTATTATGTCAGAGTAAGACAAGATGTTTTAAAAGATAGTGAAGATCACACTATTAAAATCAAGGATTGTGAGGTTTGTTCTATCTAACTATTAAAGCAGTTAGAATTTGTTAGAATTACTTGTTTTAAAACTATCTTAATCCCTAATATATAAATTAGAAAGGCAACGGTTTTGAGAAGATGCATGCTATTGTGGTTTGTGGTGCTAAGCAGTATTTAGTCCATGAAAACGAGTCTATTTTTGTTGAAAAATTAGCTGGTAAAGTTGGTCAGGAGATCCAACTTGATAAGGTATTGATGCTTGATGAAAAGATAGGCAAACCTTACCTTGAAAAAGCTAAGGTTGTTTGTGTGATTGAAAAACACGGTTTAAAATCGAAAATTAAACTAATTAAACACATCTCCCAAAAACACCACCTCAAGCGTTATGGCCACCGTCAACCCTACACCAAACTAAAAGTGGTACGCTTTATCCATGATTAAGATTAATATCTCCCAAAACTTTCTAGTTGCAAAAGGTCATGCTTTGTTTGCTGAGAAGGGTAAGGACATAGTTTGTGCTGCAATTAGTGGAATTATCTTTGGGGGGGTGGCTTGGTTTGAACCTGATAAGATTGAATTTACTGAAAATAAATTAGTACCTAGTATAGCACTGAAACTCATTGACCCAACCCCTAATGTAGCAGTTGCTTTTAGTGTTATTACAGTACAATTAAAAGCAATAGCCAATTCCTATCCTAATCACATAGTTATCAATGAAGAGAGTTATGAGTAAAAACAGTTACTGCTACCAAATTAACTTACAGTTTTTCGCTTCCAAAAAAGGGGTTGGTTCCACTAAGAATGGACGTGATTCACATTCTAAGCGCTTGGGCGCTAAGAAGGCAGATGGTCAGATGATTAGAGTTGGTCAGATTATCTATAGACAAAGGGGAACTAAGATCTTTGCAGGACAAAATGTTGCAATGGGTAGTGATAACACCCTCTTTGCTTTGAGCGATGGCATTGTCCGCTTTCAAAAGTTTGGTAGTAAACAAAGCAAAACCCGGGTTAACATCATCAAACACCAACTAAATGCCTAAGCTACTGGGTAGCTTTATTAGCTTTAAAGCCCCCAATTACTTTGTTCAAAGTGCTCAGGATGCAATTGCTATTGATGCAACTGCTCTAATGGTATTTTTAGGTCCACCCCATTCTGCTTACCGTGTTCCTTTTAACAAGATGCAGTTTAGTTTGGGCTATGAGTTGTTAAAAACAAAGAATATTAATAGTAATGGTTTGGTTGTTCATGCTCCATATATCATTAACTGTGCATCAAAAGACCCACTAAAACAGCAGAATGCTATCAGTGTTTTAACCAATGAGATTCAGCTTTGTAACTTGGCTGGTGCTCACTATTTAGTTTTGCATCCAGGTTCTGCAGTAGCCCAAACAACCAACGAAGCATTAGATAACCTGGTTAAAGTACTCAATCAAGTTATCAATAAAACCAAAACAACAGTTATTTGCCTTGAAACAATGGCTGGTAAGGGTAATGAGATAGGCAGAGACTTAACTGAGTTGAAATATGTTATTGACAGGATCGTTGATAAAGATAGGATCGGGGTTTGTCTTGATACCTGTCACTTCCATGATAGTGGGATTGACTTTAGTGATCTAACTGGTGTTTTTAACACCATTACAACTAAACTTGGTTTTGAGTTTCTCAAAGTAATCCATTTAAATGAATCTAAAAATAATTGTGGTTCTAAAAAAGATAGGCATGCTAATATCAATGCTGGGATGATTGGTTTTGAGAACTTAATGAAGTTCATTAGTCACCCCCAAATTAAGGATTTACCTATTATCTTAGAAACCCCTTCAACTAGTTTAAACTACCCAACTATTTACCGTGAAGAGATTAGTCAAATCCGCAGCTGATTTAAAACTTACCAACCAGATGCTAACTAGTTATGTGAAGGTATTAGAACAAAACAATCTGCGCTTAACAAAACCGCGGATTGCTTTATTAAAGTGTTTAATTGAACACCAAGATTGGCATAATCTCTCCCAAATTAAAACCCACCTTGATTTAGCCAATCAACCCTCAACACTCGCTTCTATCTACAACAACTTAAGAATCCTAGCTAAACTTAAACTGATTAACATCTTTGTAGATCCAGAGCGGTTTGAAACTTACTATTGCCTGCGCCATGCAGAGCATAACCATATCTATCTTTTTGATGAAGTTAAACAGCAGTTTTTTACCTTACCTTTAACAGATGGCCAAATTAAAACATTGCTTGAAACCCAAAACCATACCAGTAAGGTAAAGCTTAATGATTTTTATATTGTTGCTAGGGGTGAGATAAACAATGATTAACAAACCAAACCAATTTTTAAACCACCTTGATGGCTTAAAGCAACATTTTTCTGATTATGATTCACTACAAAAATCCTTTAAGAAATATCTATCAGAAAATCAAACTGAACTTAACAATTTCTTTTTTAACCAGTTTGAAAAGATCATTGTTCTGGTCAAAAAAAAGGAGTTTAAAACTGCTCAAGAGAGGTGTGAAGAGGAGTTAGCTACCCCTTATTTTTCAAAGCCATTGGTTGGTTTTTTCCAATCACTATTACAACTAATTAACCATGATCTCATCGAACAGAAAAACCAACAGTTAGCTAACATGAGTTGTGAAAAAATTGTGGAGATGGTGTTAAGTGATTACCCCAATAAACTTAACTTAATCCACTATTTGTTAGCAAAAGAAGCGAGTTTTGTAAACCCTAACCTTTTACAGCGGATGACTTTTGTGTTAACTGATCTTGAACTGTTAGAGTTAAAGCGTTTCTCTTTTTTTAAAGCCCTTAACCAGATACCTGCTTTTAAAAACCACAAAGTAACATACTTTAACAGCAAACTCAAACAGAAGTTTGTAATAACATTAGGTGAATTTGCTTTCCCCCAAACTGATAAAACCAAACAGTTTTTCCAACAACTAATTAAAAAAGTAAGTCAACTGTTTTTAAAAGAACCTGTTAGTTGTGAATTTGCTTATGAAATTATTGATGCATTACTCGTCAGTTTTTTTCCACTCCATCCTAATTTAGAAGTAAACCACTTAGCTAAAAAGATCCACCAGTATGTTAGTAAGATTGTCATTAATGAAGTTGTTGATCTGAAAGATCCAACCACTAAACTAATTGTTGATACACTTTATGAACAGTTAGATAGAGCAATTGGTGAGGAAAATTAAAATTAAGTTAGCACTAGTAGATACAAAAGATGAAGTTATACAAAGTTCTTAACAGTAAAACAACTGATAAAAGTCTTTGTTTGGAAGTTGAGATTGATCCAAATTACTGACAAGCTACCCAAAAAAAACTAGTAGGTGAAATGGCTAAATCGATAAAAATTAAGGGTTTTCGTCCCGGTAAAATCCCCCCTAATTTAGCCAGTCAGTCGATTAATAAAGCTGAATTAATGCAAAAAAGTGCCCAAAACGTCATGAACAGTATTTATGAATCAGTTCAACAAGAAGAGATCGTTGCTAGTAATGATAATGTCATTGATGATTATCCTACCATTGATTTCAAAACGATCACTGAACAAAACTGTGTACTTTTGTTTTACTTTGATCTGATCCCTAACTTTCAACTCCCTGATTACAAAAAGATAAAAGATTTAACACCACTTACCAAGTTAACTGAAGCTGAATTTAACAACGAAATTGAAAAGCTGGCAAAAACTAAAAGCACAATGGTAGATGTTAGTGATAAAAAACTAGCTAATGGTGATATTGCTATCATTGATTTCACTGGGATAGTTGATAACAAAAAACTAGCATCAGCTTCAGCACAAAACTATGAATTGACAATTGGTTCAAATAGCTTTATTAAGGGTTTTGAAACCGGGTTAATAGCAATGAAAGTTAACCAGAAAAAAACTTTAGCACTAACTTTTCCTAGTGATTATCATGTTAAGGAGTTGCAATCAAAACCAGTTACATTTGAAGTAGTTTTAAAAGCAATTAAAAAACTGGAATTCACCCCAATGGATGAAACTAATTTCAAATCCTTTCTCCCTGAACAATTCCAAAGCTTTACTTCTCTAAAGGCATTTAAGAGTTATTTTCATAAGCTAATGGAAAACAAAAAACAAGAGACAATTCTCCAGGAGAATAACCAAAAAATTCGTCAGTTCTTACTTACTAATACCAAACTTCCTTTTCTTCCAGAAGCGTTAATTAAACTAGAAGCTAACCGCTTGTTAAAGCTCCAGCAAAGCCAAGCTGAACAATATAAAATCCCCTTTGAAAAACTCTTAAGTGCTTCTAATATCACCCTAACAGAGTTACAAGATCGCAACATAAAAGAAGCTAAGGAAAATGTTACCTTTGCTTTGGTAATGAAAAAGATAGCTGACATTGAAAAGATTAAGGTTGATAATAACAAGATTAAAGCTGAAATTGAAAATGTTATTGCTGTTGAATATCCCTTTGCTAGTGATGAAATGAAAAAACAACTCTTTTTTAATATGGAACAACAAAAGGAGTTTGTGGAATCAATTATCATCAACAGATTAACAACAACTAAAATCGTTAGCTATTCAACTCATTAGCACTCAAAGCTTGTGAGTGCTAAGAAATGTGTTAAAATTTATTGAAATTCCCTAATTAACTTTTAAATATGCCCGTTACGAAGAAAAGTCAGATCTTAGTAGTTAGAGGTCAAGTCATTTTTCCTTTTGTTCCCTTTAGTTTGGATGTTGGCAGGCCCCGTTCGCGTAAGATCATCAAAGCGCTTAAAACTCTGAAAACCAAACGTTTGGTTTTAGTAACCCAAAAGTTTACTGGTGAACAAAACCCTGAGTTTAATGACATCTATCATGTCGGTACACTCTGTGAGATTGATGAGATAGTTGATGTTCCAGGGGTTGATAGTAAAACAGTAGACTACCGTATTAAAGGCAGAGGTTTACAACGGGTTTTAATTGAAAAATTCTCAGATGCAGATATTAATGAAGTTAGTTACCAATTACTTAACTCCACAGTTAAAGATGAAGCTAATGTTGACAGGTTCTTACAGCGAATCTTTCCTGAAAAAGAAGAAATTGAACAGTTAATGGAAGGAGCTGAGAAGTTTTTAGAACTTGAAAACATCAGCAAAACAGTTAATGTTCCTAAGGGTTTAAAGCAACTTGATATTATCACCTTTAAACTGGCTAATCTTGTCCCTAACACTGAAAGTATTAAACAAGCTATCTTAGAGGAAAATGAGATAGCAAACCGATTGGAAAAGATTATCCAAGCAGGGATTGAAGATTTACAGAAGATCCAAGATTATGGTAGATCTAAAAACAAGGAAACTGAGTTTGATAAACTTGACAGTAAAATTACCCGCAAAATTAACGAACAACTCTCAAGACAACAACGTGATTTCTATCTTCGTGAAAAGCTAAGAATTATCCGTGAAGAGATAGGGATTAGTTCCAAAAAAGAGGATGAAGTTGCTAGTATTAGAAAGAAACTGGATGAAAACCCTTACCCTGAAGCCATTAAAAAACGGATTTTAAGTGAACTTGAACACTATGAAAACTCTTCCTCCTCTTCCCAAGAATCAACCTTAACCAAAACTTACATTGATACGCTTTTAAACCTGCCTTGATGACAAAAGAGCAAAGATAACAGTGATGTTAAAAACTTAATTAAGACGTTAGATAAAAACCACACTGGTTTAGATAAGGTTAAAGAAAGGATTGTTGAGTATTTAGCAGTACAACTAAGAACCCAAAAAAACAAAGGTCCTATTATGTGTTTAGTAGGTCCTCCTGGGGTTGGTAAATCAAGTCTAGCTAAGTCTATTGCAGAAGCATTAGATAAGAAGTTTGTCAAGATCTCATTAGGGGGAGTACATGATGAATCGGAAATCAGAGGTCACCGTAAAACTTACTTAGGTTCTATGCCAGGAAGGATTTTGAAAGGGATGACCCGTGCTAAGGTAATTAATCCCCTCTTTTTACTTGATGAAATTGATAAGATGACCTCCTCCAACCAAGGTTATCCTTCAGGTGCTTTACTTGAAGTATTAGATCCAGAGTTAAATAATAAGTTTAGTGATAACTATGTTGAAGAAGATTATGATCTTTCTAAAGTAATGTTTATCGCTACTGCAAACTACATAGAAGATATCCCTGAAGCTTTACTTGATAGGATGGAGATAATTGAACTCACTTCCTATACAGAACAAGAGAAGATTGAGATAGCAAAAAACCACTTAATTAAGCGTTGCCTTGAGGATGCTGATCTTAACAGTGAAGAATTGAAGTTCACTGATGAAGCAATCAGCTACATCATTAAGTTTTACACAAGAGAAGCGGGGGTTAGACAATTAGAACGATTAATCCAACAAGTTGTAAGAAAGTACATAGTAGCAATGCAAAAAGATGGCATCAAACAAGAAACGATTGATGTAAACGCTGTTAAAAAATACCTTAAGAAGGAGATCTTTGATCACACTATGCGTGATGAAGTGTCTCTACCTGGAATTGTCAACGGGATGGCATACACCCCAACTGGAGGGGACTTACTTCCCATAGAAGTTACCCATGTTGCTGGTAAAGGAGAGTTGATCTTAACTGGTAATTTAAAGCAAACAATGCGAGAAAGCGCTAATGTTGCTTTAGGCTATGTAAAAGCTAATGCAGAGCGTTTTAACATTAATCCTAGTTTGTTTAAAAAGATTGATATTAACATCCATGTTCCAGGTGGGGGAATTCCTAAGGATGGACCTAGTGCTGGTGCTGCTTTGGTAACTGCAATCATCTCATCATTAACTGGTAAGAAAGTAGATCCTACAGTGGCTATGACAGGAGAGATCACTTTAAGAGGCAAAGTGTTGGTTATTGGTGGGGTGAAAGAAAAAACTATCTCAGCTTACCGCGGTGGGGTTACAACTATCTTTATGCCTGAGAAAAACGAGCGCTATTTAGATGAAGTACCCAAAGAGATAGTAGATAAACTTAACATTATCTTTGTTAAGGAATACAGTGATATCTACAACAAGCTTTTCAGTTAGTCTTTAACTAGGTTTTGGACATATTTTAAGGTAATTAAATAGGCCTGTTTTAAGTTGCTTTTAGCTAGTTTTACTAGTTGATCAATCTCAATTCCCCCACTCCACTGTTCCTGTTTACGGTTAGGTTCTAGTTTATCTGCTAAATAAACAATCATATCTAGCTTGCTAACTTGTTTTGGTGGGATGGTGTGGTTTTTGATGGCATTAATAATCATTTTATCTTTAACACCAAATCAGTTTTTTAAGATATAAGCCCCCACATAGCTATGTAATACTTTTCAACTTGGGTAGTTAGTTATCTTCAGTTCACTAGTTGCAATGTTAACTAACTGATCAACTGGTAGTTGTTTGGCCAAGTCATGATAAGCCCCAGCAACAAATGCTCTTTTAGCATCTAGTTTGTTTGCAATTGCTAGTTGTTTTGCTAGTTTACCAACCCTTAAACAGTGTTGAAACCTCTTATCATCAACCATTGCTTTTAAAGTGGGAATTAGATAGAGGTGATTGGTATTGATGTAATTTAAAACTGCCAGTGGTATCAGTTTTTTTCTAGGTTGATTTAAAAGTTTACTTGAAGCGATTTCAAGTGGGCATTTAGCCAAGTATTTAACATTAAATTGGTTTGCTATTTTTTTATTAAAAGGATAAGGTTTTCGTTCATAACAAACAAACGTGCACAAATCTTTTAACTGTTGGATGTGATCTCACTTCTCAAGTTCATTTAATTTATCACTCCCTATTAAAAAATAGATTTCACTAGTTGGATAACAACTTTTAAAGTGGTTAACAGTATTAATTGAAAAAGCGTTTTTAGTTTTAATATCAAAATTGGAAACTAAAGCATTGTTGACTGATTTAATTGCTAGCTTTAACATAGCAATCCTATCCTTATTACTAGCATGAAAATTATTTTTAAAAATACCATTGTAAGTTGGTACAAAAAAAAGCTTTTGCGCTTTTATTTTTTTAATTGCATGTTTAGCAATGTAGAGATGGGCATTGTGAATAGGGTCAAAAGAACCACCAAAGATAATAATTTTTTGCTTCAAAAAAGATCTTAAAACATTTAATTCTAACTAAAATAATTGTGACACAAGAGCAATGATCCAAAAGGAGATGGAGATCTATAACCTCTTTACTTTTCAAATTGATCTTGATAAAAAACTGTTGTTTGAAAAATCAAATGATCAGAAGAATTATTCCAAGATTAGAACCCATTATTTCAAGCATAAATTCAAAAATAAAAGTGCTGTTTTTCTCAATAAAAACCTGATTAAAAATAGCTTAAATAAGGTTCTTTTAAACTTTTCTGATTTTGTTAGTGGTGCTGGAATTGATACAGTTTTTAACCAAATCATTGATGAAGATCCTGAGGTTTTAAACTATTTAAAGCAAGTTAAAAAAGATCTTTCCAAAGAAAACAACGCTACATCCCAACTAACCTTTAATGTAACTATTAACCCTAAAAACACCCTCGCCAATTTTTTTGAAGGATTTAATATTTATCTCCATTTTAATGAAGAAAACAATACAGTGATAGGTAGTTTCTCACTACAATGACACATCAAAAAAACTGATCTGTTTTCTGAAACAAAAAACATTGCCATTAACAATTTAATTCACACTTTTTGTAAGAACAACATGCATGAAATTAGTTTTATGCAGATCATTAATTGTTTTTCCAAAACAAAAATTAATAAACATGGTGAAATTGTTTTAAAAAGTTGTGCTTTTAAACAAAAATGACAAAATGTTGTTGCTGAAAAATACCCATTCTCAACTGCATCTAAAGATTTGGAGAAAATTAATGATTTTTTTGATGCTCTTTTTGTAATGCTATTATTGGTATGTCACCTCAATAAAAACCTGTTATGATTATGTGAAAAAACTGATTTTTTTGAATGAAAACCAAGTCAGAAAACTGCACTTTTTAAAGCTAATGATAGTGGAGCATACTTAGCTAGAATGCTGCTCTTTTTAAACGATTGGTACAACGAAAATCAAGCAATAACAACTGCTGACATTGAAAATGTTAATGAGGTTGAAGACATAGGAAAATTAGTTGAAAAATACAGCACTAATCAACCCCAAAAGTTATCGTTAAATTCAACAGTTTATGTATTACAAACAAAACAAAAACAGTTTTTTTTAAAAAATGATTTCTTTTTTAATAATAACGAAGCAAAGCTGTTTTTTCTAATAACTATGAAGCCCAATGTTTTTGGTCTTGATGATACAGCAATAGCAAATAACTTAAACCTTAAAAAAATTAGTGATTTTTTTAAAGAGATTGATTTTAATGATGAGGATATTCTAAATGACTTTAAACAAGAACAGGAAAAGTTATTGGTTAGAAGAACATTTAACCAACTGTTATTTATGAACAAAAATACTGAAATTCTCAGTGTTGTCAATGACAAGCAAAAGTCAGTAATCCATAACATTGTTTGAACCATTACTTATAGTAAAGCGATCATGTTAAAAGCCTTTGATTATTCCAAAGCCTTTGAAAAAAACCGAACGAGTGATCCTTCCTTATTGCGATCTAATTTAACTGTTATCAACCGCTTAAGATACCTCAGTGAATACTTTCAAAATGCCTCACTTAAGTATGATCTACTCTACACTAAGGCCAAACAATATATGCAAATTGATAAATTTATCAACGACATGATTCGCAAGGTAAACCATGAGGATGAAATCTTTGGCAAATTTAAAGAAAGAATTTATTTAAGTTTGGGAATTATTAGTGCGGTAGTGTTTGGCATAGTTGAATTTTTTAACTGTGTATGGACTATCTTAACTGTTAGTCAAGAAGTGGTTGATAAAAGTGTTTTGGATCCAAGGAATATTATCTTTATTAGTATAGGTACTATTTTGGTTTTATTTCTTTTGGTTACTATCTTGGTTTTTATGACAAGAAGACTTTATCTGTTTGAAATTAATAAAAAGCATAAAAATTAGGAATCATGAAATTCAATAAGTTAAACCTAAGTCATTGTATCTCTTTTTACATCTCAGAAGTTTCAGAAGTTTTCTTTGAAAGTATCAACCAACACCCTAGTCGTGATTTTGTTAATAACATTCTTCAAAAGATTAAAACTACTTTAAGTGAAGAAGAGCTTGAAAAGTTAAACAGTATTGAAGAAGTTACTAAGGATGAGAAGATTGTGATCATGCTCAACCATGTTTTGAAAAAGATTGTTTCTAAAACTGGTAGTTCTAAGTGTGACTTATTTAATGTAATCAAACAAGATAGGTTTAATTCTCCAGTGTATATCCAAAGTATTAATGCTTTTGAAAACAACCTTATTAACAATGAATTTGCTGAAAGAAGGTATGACTATTTGATTGAAGTTAATAAAAATTCATACCTTAAAAAATTTGTCAATTCGATCAGAATTTCCTTCTTCTTAGATTTAAGAGCACAAATTTTGTCAGGTTCATTTACCCTTAATTTAGTTAATAAGTCAATTGAAAAACAAAAAAAGACAGAAATTTTTAAAGATATTTTTGTTAATGCCTTAGTTAAGCATTTTATCTGTAATCAACTTTATCCTATCTCTTTAAATTCCTTTATTTTTGACAGTGAAAATCCTAGCAATAAACTCGCTCTAAAAGAACGGATTAAGCTTTTAAAAACAAACTGAAATTCCCTCTTTTTTGATAAGTTTTACAACTGTTTAAACAATAAAAATAAGCAGCAACTTCAAGAAACTAGTGATGAGATGTTTTATGCAGTGATTAACACTTATTTAATCATGTTAATCTCTGTTGAAGAGTTAAGGGTTTACTTTACAAGTAAAGAACCAGCACTGATTTTAAAGGTATTAGATAAAAAAAACACACTAAGAGAAGATCCTGATCAAAACCCTGAAACTGATTTGTATGAACTAATACAGTTTATAGAACAAAACTACTTAAAAAAAGATAAAAAAACCAGCTGAAATAAAAAAAAGGTACAGGATCTTGAACAGTTACTTGAAGAGATTAACAAGATTAATTTAGAAACAAAAAATGAATCTTTAGCTTACCCTGATGAGATAACAGAATTGGAAATTGATAATGATAATTTTGTCTCTACAAAACAAGTATTTAGAAACCAATTAGAATTACAACTTTTGCATGGGATTGTAATTAATCCTGAGAAGTATGGAATTGGCATGTGAAGTAGTTATTTTGCTGATTGAAGTGAGTACAAAAATTTAATAGAACAGATGCTCAATCCCAAAAGTGGTAATGATTTTTATCAGTTTGAAAAGGACATAGATGAAAGCATATGTCAAATTAACAAAAAATATCTAACTTTTATTAGCAGTGATAGTAATACCTTTTTAATAGTTAAAAATGATGATGTAAAAGTTATTTCTAACTATGTATGAGCACAACTTTTCTTTGAAACAAGAAGGTGAATTATCAATGACATTGAATTTGACTTGTATGAAAAAGGATTTGATAAAAGCCACTTTTCACGTAACATTGCTTTACTTGAAAGTCTCAGTTTTAGTTGATTAGATCCTTTTTATGGTTTGACATCAATTAAAGAGATCATGCAGAAAATAGATAGTAAAAGTAATCTTAAAACCTCGATTGAAGAGATGGTAAATAGGTTTAAACATGAGCAGCGAATTAATAAAAAGGACAATGAAAGGGTGTTGATGATTTTTGCTTATATTGCTGCTTTTGTAGTAGGATTTATCAATTTTTTCTCAATGGTCTTTACTATTCTCACTGTAAGTGATCTAAATGCTGGGCTTACTGTACCTAACATCATTGTAATCAGTATCGCTAGTGTTTTAGCTTTTATTTTGATTGTGATTGCTGTTTTATTTCGTTTTAAATGAAAACACATTAAGCACTAATAATGCAAATTAAAGTTATTAATGAAACAAATAAAACTGTTCAAATTTTTCAATGTGCTAAAGTAAAACATAGGGCATTAGCTTGATTGTGCGATGTATTTTTATTAGCTATTGTACTTGTGGTTATCTTTTTAATTACTCAAGCCTTTAGTGATAACCGTTTTCTTTTATTTTTAGTTTTAAGTTGTTCACAAACAATCCTGTGAACGGTTTATTTCATTTTTTTACCATTTTTTTGGGATGGTAAAACTTTGTTTCGCAACCTTTTGAAGATTAAGCTATTTGCTTTTGACAAAAGGTTTTTGAGAATAATGATCCATGAGTTGTTTTTATGGATTTTGCTTTCAGTTTTGTTTTTAGTGATTGCTAGTTATTTTTTTATTAACCAAAATAGCAGCGAAGCGCTTAATTTTTTCACTAATTTAGATAAGCCTAATGCAATAGCGATAACAATTAGAACCATAACAATTTTGATTAGTTTTTTACAACTGATTTTTATTGGTTATTTTTGTTTTAGTTCTGAAAAACAAGCTTTACAAGAGATACTGTCAAACACCTTTATGGTTCAAGAAAAACACACTTTAAAATCAAAACCAACTAGCTTAAAAACCAACAATCAACCTGATCCAGCTAATTTACCAGGCGTAATAGCTCTTGATGAAGTGGAAAAACTCATTAATTAATAACAATGAATGAACAACAAAAACAAGCAATTAGTTGTGGAAAAGGGGTTAATGTTGTTTATTCTGGAGCAGGTACTGGTAAAACAACAATTATTACTAATCGCTTTGCATACTTGGTTAATAAAGAAAAAGTTGATCCTAGCAGAATTTTAGCAATCACCTTTACTAAGAAAGCTGCTAAGGAGATGCAGTTTAGAATCTTGAAACTAATAGATAGTTCTTTAGCTGAGAAAACAAATATCTATACATTTCACAGCTTTTGCAATAAGTTTTTAATTCAAACATTAAAAAAGCGCTTTATCATCGATGATGATATTAGCTATTTCCTAAAGGAATTTTTAGCTGATTCAAAACTCGATATCAACCTAGCGAAACAAATTATTGATAACTTTAAAAATACTTTTGCTGATTTTGAAATAAATAAGTTGGATCAAGATGAAAGGTTAATTAGTTTATGTGAGCATTCACTTCTAAATAAAGATGAAGAATATTCCACTTTAAAAACCCAACTGATTAATGCATTCATTAGCTATGAAAAGAATAAGATATTAAACAATAAACTTGATTTTCATGATCTTTTAATTAAAACTTGTAATTTATTGAGTAATGATAATGATTTACTTAATCAGTGGAGTGAACAGTTTCAGCATATTTTAGTTGATGAATTTCAAGATACCAACCAAATCCAATATGAACTGATCAAGATGTTAGTAACTAAAAATAAAAACTTGTTTTTGGTAGGTGATAATAACCAGATGATTTACCGCTGAAGAGGGGCGGTAAACGGGATCATAACTGCTTTAAAGCATGACTTTAATGTTCCGAAAAGCAATGAATTCTTTATTAATCAAAATTACCGTTGCGATCAGAATATTTTAGCAGTTGCTAACCAAATTCTTTTAAAAATTATGGCCTATGAAAAACAAGTTAAAACTGAAAAAAATCTCTTGTTTTCAACTTTAAATTCTGATAAAAAACCTGTTTATTTTCAAGCTGAATCAGTTGAAAATCAAGCCAATTGGATCTTCAATAAAATCAAAGCACTAAACCAAACAGAAAAGATTAATTTTAAGGATATGGCCATCTTGTTTAGAAAGAACAGAGATATTACTACTATGGTTGAATTGATTGAAGCGGATGGAACAATTCCCTTACCTAAACAAAAGAGTTATTTTAACCAACTAGTAAAACTCCAGCGGGTTTTAATTGCGATTTCAACCAGAACAAATCTTGATATTAAAAGAGCTTTGCAAGCCCTAAAAATTTGATCAAATGATTTAAAGGAATTGTGAAAACAGAGTGATAAAACAAACCTATTTGATTTTCTTAAATGATCAGAATTAAATCAAAAAAACCATAGTTCAAAACTTAAAGCTACTGGTTATTTTAATCTGCTGATTAAGTTAGCAGAGGATCAGCAAATTAACCTTTTGTTTACTGAACTGTTTAAAAAACTCAAAGTGGATCAAACTATTGAAAATCTGCTTTGAAAAAAACTAACTGAATTTCAAAAAGATAAAACTGAATTTAGCTTATCAGAGTTTATTACTAGCTTAGCATTGGAATTTGACTCAATTATTGAAAACAGCAGTGATACAATCAATTTGCTAACCGTTCATGCAGCAAAAGGACTTGAGTTTGAAGCTGTATTTATTTATGGCATGAATCAAGGGGATTTTCCCTTATTTTTAAGTCAAAATCAAAATGACGAACAACATTTAATTGATGAATTAAAACTGTTTTATGTTGCTATCACAAGAGCAAAACGTTTTTTGTTTATCACTGCGGTTTTACAAATAAATAACAATTCTATAAAACCATCTAGTTTTTTAAATTACATCAATAAAAGTGAGTATTTAGACATTGCTACTATTAACTATGTATTAGAGCAGGATGATGATTTTTTTGATTCAACTAAAAAAACAGACTATACAAAGAAACTAAGAAAAGAAAGTTTAGACATTATAGTGGGTGATTTAGTTACTAGTAGATACTTTGGAAAAGGAGTTGTAGTTGAAGTGAGAGACAAAGAGGTTTTAGTAGCTTTTAAAGACACACGCTATGGGATGAAATGGATCTTAAAAAACCATAAATCACTAACAAAAGCTTTATATTAACAATGGTTGATAAAAACAGTTTAAGAAAATTAATGCTTCTAAAAAGAGCAGAACTAAATGATCTTGAAAAATCGCATTTAGATCAAAAGATTAACCAAAAATTAATGGCTTTTTTAATAACAAGACCAACAATTAAAAATTTAGCACTTTACATTCCCATTAAAAACGAAGTGGCTTTTTTAGATAACTTTCTAGATTTTCTTAAGTTAAATAAAATTACAAGCTGTTTTCCTAGTATTGTTGATCAATTTAACATGAAGTTTATTGATCAAAATAATAATGAAATTAACCCTAATGATATTGATTGTTTTTTTATCCCTTTATTAGCTTTTAATAAGGCAAACCACAGGATTGGTTTTGGTAAGGGTTATTATGACCGTTATTTATCATTAACTAGCAAAAAACAACTAAAAATAGGGATAGCATATGACTTTCAATATGCAGAATTCACTAATGATCCTTGGGATTATCAATTAGATTTAATTATTTGCAATGGATAACATAAAGGTTCTTTTTTTAGGTGATGTTTATGGCAAAGCTGGTAGAAAGATTATTAGTGATCATCTTCCCATAATTAAAAAAAAGTATCAGTTAAATCTAATTATTGCAAATGCTGAAAACACTACTAATGGTAAGGGTTTAAGTTGAAACCACTACCAAATACTAAAACAAGCAGGAATTGATTACATCACTATGGGTAACCATACCTGGTTTCAAAAGCAAGATTTAGAACTTGTTTTAAACCAAGTTGATGTTATTCGCCCACTTAACTTAATGCAAGATTTTAACTATTTTCAGCTTGGCAAAGGGAGTTATTTATTTAGCTTAAATGGTTTGAAAATAAGGATTACTAACTTGTTAGGAACAAGTATTAACTTACCATTTGCAATAACAAACCCATTTGTGGAATTAAAAAAGTTAGTTTTAACTAAAGATTGTGATCTTCATATCGTTGATTTTCATGCTGAAACAACTAGCGAAAAAAATGCTTTTTGCATGGTTTTTGATGGTTATGTTACTGCTATCTTAGGAACCCACACCCATGTTCCTAGTAATGATTTAAGAATCACTCCTAAAGGAAGTGTTTACATTACTGATGTAGGGATGTGTGGTCCTGGATTTGGTAGTGTTATTGGTGCTAATCCCAAGCAATCAATTAAGTTATTTTGTACAGGTGAAAGACAGTTTTTTGAGGTTTCTAATTGTGGTGCACAACTCAATGGCGTCTTCTTTGAAGTTTGTTCAAAAACCAATCAAGTTGTGAAAATAGAACAGATTAGAATAGTTTTAGATGATGAAAAGTATTTAGCTAATGACTACTTTAATTTAGTTGAGTAATCTTGCTTTCCTTACCATGTAAAATTCTGATTATGTTTGCACGGTGTAATCAGAAAACAACCACAGTTAAAGGTCAATATCACAAGCAAAAAAATAAAATGATATACCATTCATTTTGATAAGTAATCGACTTTAGAGGATCACTGTTAAAGAAGTATAAATAATCAAGTCAGGGTATTAAGATGATTACAGCTAACACAAAGAATGTAATAAGACTTGCTAAAGAAACATACTTAGTTATTAAAGTAATCATTATCCAAATTAAAAGACAGATTAAAAATCATCATAAAGATATTGCAAGTAAAGATCCACCAGTAGTAGCAATTGCCTTACCACCCTTAAACTTAAAATACAGCGGAAAGATATGACCTATTGTAGCTGCAAAACAACTTAAATAACTTAAAAAATAGGTGCTTTGATACACTTTTTCTGTTAAATAACCTTGTAAACCAAAACGGAATAAAATTCAGGTTAATAAAAAAGCAAAAAAACCTTTAAATGCATCAAAAATAGCCACCAAAAAACCAATTTTTAAGCCAAAAACACGCATTGAGTTAGTAGCTCCTGGGTTTTTTGAACCAAATTCCCTGACGTTTTTCTTGAGTATTTTGCTGAAAATATCAGCAAAAATAATTGAACCTAACAGATAACCAGAAGCTAGGCTAAAAATGACCAAAATGGCAATTGCACTAGCTTGATTCATAGCTTGTAATTATCTTATTTAATGTTTCAATTATCTTAAGATCCATCAAGCTAGGTTTTTGTTTGGGTTGATAAACTCTCAATAGGCATTGGTAGTGTTTCATTAAACAGTGTTTATCTTTAAAGTTAATTAGCTTTGGTCCTAAAATAACCAATTCCTTTGTTAATTTAAACGGTTTTTTTAGTTTTTCAATAACCAAAATAGGATATATAAACTCTCGATCCTGAACTAGTGTTTCATTAACAATATCCCAACTGTTTAAACTTAAAAAACTACGTAATTCAATTAAATTAGATTGGGGTTGGATTACAAAACGATTAATAAAGTTTTCTTTTTGGCTAATTATGTTAATGATTTTCAATCCACCAAGTCCAGCAATTACCCCTATTTTGGGATTAATATTTAGTTCTGGAAGGTTATTAAATCCATCACTAACAAAGAAGTGGATATTATTGTTATTTTTAAATTTTTGGTAATTACTTAATAAAGCATTTTTACTAATATCACTGTTAACAATGGTTAGGTTTTGATTAGTTTTTATTAGATAGGATGTTAGATAGGAATGATCACAACCGATGTCATACACTAGCTTAGGATTAAATGATTGAACTAAATTAGCAATTGTGCTAATCCTTTTTTTCATTAATTCTTAGAAATGACATACTAATAGGATTGTTTCTAACTGCATGTCTCAATTTTCTAATTGCTTTGTTTTCAATTTGTCTGATCTTCTCTCTAGGGATCAAAATCTTTTGACCTACTTCATCAAGTGTTTTAGGTTCATTGTAAGGGGGCATGCCAATCCGCATTCTAACAATTAACTCTTCTTGTTCAGAAAGATTATTGTTCAACAATTCATCAATTTTTTCTGAATTTGAACGGCTTTCGGTAAACTCGTCAGGAGTTTGAGCGTCTGTGTCTTTAACAAAATCACCAAACTGGGACTCTTCATCATGTCCAACTGTTTTATCAAGCGAAACTGGATCTAAACTTAACCGTTTAATTTCAGCAATCTTTTTAACATTAAATCCTTCAGCTTGTCCTCCCATCTTTTCAGCTAACTCCTCATCAGTAGGTTCTCGCCCTAACTCTTGATACAAAGCCCGTTCTGCTTTAGCTAAGCGGTTAATGGTTTCTACCATATGAACAGGGATCCTTACTGTTCTTGCTTGATCAGCTATTGCTCTTGTAATTGCTTGTTTAATCCATCAAGTAGCATAAGTTGAAAACTTATTCCCTAAAGATCAGTTAAATTTGGAAATAGCTTTTAAAAGCCCCAAATTACCCTCTTGAATTAAATCATTAAAATCCAACCCTCTTTCTAGGTGTTTTTTGGCAATAGAAACAACTAGTCTTAAGTTTGAAGTAACCAACTGATTAATTGCATACTTACGTGACTCTTCATCAGTACTATTTAAAACCTTGGCAATCCGCTGTTCAGATTCAAAATCTAACATTTTAGAAAAGTCAAGTGATCCTAAAAAGAACCTAACATTATCATCAACCTTATCACGGTTAGAGATGTTCTTACTTGTTAACTCTTCAATATCTTCATCAATAATTGAAAGGTCTTGGTTAGCACGAAATTCATGGATGTGCTCTTCAACATCGTGTTGGAGTTGAATCCCCTTATCACGTAGTTCATCCAAAACATAGATAATTTCATCTTCAGGTAGTTCAAACTTAGCGAGAACACTAATAATTTCCTTATTTGAAAGCGTGATGTTTTTTTGTTTCTTTTTGCTTTTCTTTTCTCAAGCTTCTGTTAGAAGGTTGATGACTGTGTTGGTGTCATCATTTTCAATGTGGCTTTTTAACGATCCTTCTAAGATATCAAATAACTTCAGATTATTTTTTTCTTTTAAAGGAGCGTGTTTAGGTTTTCTACCCCTTCTTTTTTTAGGAACATCACTCTCCTTATTTTCATGAAGGGTTTTGAGAAAAGCGATGTTTTCATGACGGTGTTTGCGTTGCACTTTATTGTCAACATAAATCATCTTTTTGTAAGGACGATTCTTTTCAAGAATACGCTGTTTTTTAAGTTCAGCAATTAATTCTTCAGATAGTTCTGGTTTGGTTGAATTGGGTTTTTCGCCTAGCGTTTTTTTGTCAGTGGACATTTAGCTCAATGCGACTAGCATTATAATTTTAGCTTATATAACTTTTATAGCGTTAACTTTAAATTTTTTAAGCGCTGCTTTTGTTGTTTTAATAACGTTTGAAAGGTCTTTAGATAGTTTTGAAAATCATTAGCTTGATAACAAGTTAATTCATCTAAAAAAATATCTTTAATTTCCAATAACCTTGCTTTATTGTTTAAAAGGTAATTTTGATCTAAAACACTTTCAAAACCAACTCAGTTAGCAGTTTGATTTTCACTCCAATAGATCCTTGCTTTTTCAATAAAAAGTTCAACATCAAACAAATTAAAGTTACATTCCTTAAAAACTTCATCTAGATGGGATTGTTTTAATTCAACCAAAAAGCGTTTATCAATTAAAGCAAACGCAAAGATTTCAGGTTTTCACAACTCATTAACTGCTTCTTCAAAAGCTGTTTGCACTAGTGCTGCAGTGGTTTTTTGCAATTCTTGTGGTGGTTGAGCATGGCCTGAAGTATTTATGTAAAAACGGTTGTTATCAAACTTAGATACAAGTTTTTCTTTGTGTTTTAAAACTGTTTCATACAGAGTTTTTTCATCACTATATTCAAGTAGTTTAACCAAATTTTTAATGAGAAAAATTAAAAAACTATGGTCGTTTAAAATTGTTTGGTTTTTTGTTAAAAAAGCAATGATTTTATTAGTAATAACCCTTTGATCAAGTTGTTGCTTTTTAAAAAAACTAACAAGATATTCAATTAGATTTTGTCTTTTGTTTGCTTGTAATATAACTTGCTCACTACCCTTGTTTAAATACAGTTCATCCCAATCTTTATAGTTGTGTTCTCACTGAACAATTTCCACAATAAAATTGTTGTTATTAAGTTTTTCAATTAAGCTAAACACAGCATTTTGACCACTAGCATCATTATCAAGTGCTAAAACTAAGGTTTGTAACTCCTTAAAGTGAGCTTTAATGGCTTTAATTTGCACATCATTTAATGCTAATCCCATTAATGCAACAGCTTCAAACTTGGAGTTTGTTAGTGTAAAAACATCAAAATAACCTTCCACAATAAAGAGTTGATTGAGGTTTTTATTTAACCTGTGAAAGTTAAATAACAGCTCCCCTTTTTTAAAAAATTCGTGATCAGCACTATTTTTATATTTCAGTTTGTTGATGTTATCAACACTTCTTGCTGAAAAACCAACCGGGTTACCATTAAAGTCATGGATAGGGATCATAATCTGGTTTTGAAAGGTAGCTTTTTTGGTATTAAAGTCAAAAAAGCCAAGACCTTGCTGGTTAGTTTTTGAAAAAAGATACAATTCACTCGGTTTTATCTTTGGATTAATGAAGGGGTATCTTTCCATACTTTCACATAGATACTTATCTTCATTGTGAAAAGCAAGTCCTAGCTGAAACTGTTCAATTAATGTTTTATTAAGCTTTCTTTTTTCAACTAAATAATTCATCCCATTTGGGTTTGTTTCTCTTTTTAATCTGGTTTGATAATAAGTAATTAAAGCATTGTTTATCTCCCAATATCGTTTTTGTTTTGGATCAACTTTTGTTAGTAAATTACTGTTCCAATTTTCTAACTTAATTCCACAAATTTCAATTGCTTTTTTAAGTGCAGTTTTTCAATCTAACTGGTCATGCTTTTGGATAAACGCTATTCCGTTGCCAGCTGCATTACAAGCCCAACACTTAAAGATGTTTTTAGAACTGGAAATGGACATAGAAGGATTTTTATCATCATGAAAAGGACATAAAGCAAGTAGACTATTACCCTTAGTTTGGATTTTAACCCCGTAGTGTTGAATAATTTCAGTAATTTTAATCTGCTTTAAAAGTTCATCTAAACTGTTGCTTTTATTGACCATTTTTCATGAATCATTGTGGTAATTCTTTGATAGCAATTCGCTGTTGAACTAAAGTATCTCTATCTCTGATGGTAACTGCATTATCTTCTAAACTTTCAAAGTCAAAAGTGACTGCATACTTTATTCCAATTGCATCTGCTTTTCGATACCTTTTACCAATACTACCAGTTGTCTCAAAGCACACTCTTCACCTTGTTTTTGCTAGCGTTTCAAATACAGAAAATGCTTCTTTTTTAAGTTTATTTACAAGTGGTAAAACAATAATTTGTTCAGGACATAAGTCAAATGGTAAACTCAATACTTCCCGTTCTTCCTCATTAATAATTTCTCTCCTATAACTACTGACAATTAGTGCATAAAATAACCGTTCAATGCCAACCGCAGGTTCGATTATGAAAGGTAAGAAATGTTGGTTTAATTCGCTATCAAAAAAACTCATGCTCTTTTTTGAAAACTCTTGGTGTTGTTTTAGATCAAAATCACCCCTGTTAGCCAAGCCTCATAACTCCTTTAATCCGTGGGGAAAATTAAACAAAAAGTCAGTAGTTTTTTTGGCATAATGAGCCAATTCTGATTGATCATATTCATGTTTTTTTAACAATTCTGGGTTAATTTTTAACACTGTTTGTAGAAACTGTTCTACCATTATTAATTGTTTTTCAAACAGCGAATTTGCATCATCAGGTTTACAAAACCACTCAATTTCAAACTGTTCAAACTCTCTAGTTCTAAACAAGAAGTTACCTGGGGTGATTTCATTACGAAAGCTTTTACCAAACTGGGCAATAGCAAAAGGTAAATTACGCTTCTTAGCTTGCAAGATATTTTTAAAGTTAATAAAGCTACCTTGAGCAGTCTCAGGACGGAGAAAAACAAGGCGTTTATCGTTGTTTACAACCCCGATTTCAGTTTGAAAAAGTAAGTTAAAATCCCTCACTTCTGATCAATTTTGGTTATGACAATTAGGACAGTTAACTTTAAAACTATTCCAATCCTTTTTAGCTTTTATTTCAGCATTAATTTGATCAACACGAAAGCGGTATTTACAACTCTTACAATCAACTAAAGTATCAACAAAGTTAGCTAAATGTCCTGATGCTTTTCAAACCAATTCGCTGAGAATAATAGGGGTTTCAACTAAAAGAACATCAGCTTTATTTTTAATAAAAAAGTTATATAAAGCTTGTTTGATTTGTTGTTTTAAAACTGCACCTAATGGACCAAAATCCCAACTATTGTTTAAACCGTTGTAAATTTCACTACTCTGAAATACAAAACCATGTTGTTTGAGAAACTGAACATAAACTTCTTGGTTGTAAACTTTAGCCATTGTTAATTAATCTTAAAAATTAGTTTTTTCAAGCAAACAAGATAATCCCTAAAGCAACACTTACATTTAAAGAATTGATCTTATTGTTCATAGGAATTTTGATTCTACAATCTGCATTCTTTGTTATTAGTTGATTCACACCCCTATCCTCATTGCCAACAATTAAGATTTTTTTAGCAAAATCAACTTTGCGATAATCAATTGGTTTTCAAATAGGATCCAGAGTTGAAACAACAGTTCAAAATCCTATTTCTTTTAGCTTAGTGATCGTATAACTTAAGTTAGCTACTTGCACTAAGTTTTGATAAAAAACACTACCCATACTAGTTTTCATGACTGTATTGTTGATAGGGACTTGGTTATTCTTTTTAAAGATAATTCCATCAACCTCACTAGCCAAACAAGTTCTTAGTATAGCACCAAAATTATAAGGGTCTTGGATCTCATCTAACATCACAAGTGTACTGCACTTCTTATTTTCAACTACTTTAACCAGTTGATCAAGTGGAATTAAGAGTTGGTTAGTATCTAAAACTGCTACCAATTCCTGGTGGTTAATATCTCTATACTGATTGTTAAATCAGTTAGTTGAATGGATTTGAAAATTAATTTTTTTAGCTTCAATTAAAGGTATTAGTTTTTTGTGACGAATGGAAATGTTTACCAATTTAATGTGAACTTGGTTATTAATCGCTTCCTCAAAGGCTTTAACACCAAATAAGCAACTTTGTTGGCGTGGTTTTTTCATTATTACAGCAACATTTTTTTAATTAGTTTTTTTCTAATTTGATCAGCTTTATCCATTGCTTTTTGTTTGTGTAATATTGATCACTCTTTAATTAAACGCACATTAAGTTTAGTATGGATAGATTTAAAGCTAATTCCTAATAAATCAATTGATCATTCCAAGAAACTAAGTTGTTCTCTCAGCACACTAAAGTCCTTAGTTTTAATACTTGTATTAATTTTTTTTATTAGTTTTCAGATAGCAGCAACTGCATTGGCAAAGTTGAGATTATCAAGTAAAGCTGAAAAAACTGGATCATATTGCTTGGGACTAATCAACTCAGATTGTTCTGAATAAACTAATCAGGTTCTAGCAACATTAAGTGTTTTTGCAATCCTTTGAATATCATTATTAGCTTTTTCAATCAATGATTGGTTTAGATCAATAGGATGCAAATAGTGTTTTTGGTAAAAGATCCAACGCAAAACACGAAAATCATGAAAGTTAAGAAAATCAACTGCTAACAAGAAGTTCTGCAATGACTTTGACATCTTTTGGTTTTCAATCATCAAATGACCAATATGCATCCAATGTTTGGTAATGGGCTGGTTATATAAAGCCATGTGTAAGGCATTTTCATTTTCATGGTGGGGGAACTTTAAATCAACTCCTCCTCCGTGGATCGTGAGTTCATTTTTGAAACAATAATCAATTAAGAAGGCACATTCAACATGTCAACCTGGTCTGCCAAGTCCCCACTTACTATTTCATTTAATCCCTGCAGTAGTAATCTTTCAAAGTACAAAATCCAATTTGTTCTTTTTATCAGTATCAGTTTCTTCTAAATGCACCATTCGGTTAGCTAGATAACCATATTGCTTTAGTGAATTAACTGCAAAATAAACGTTGTTTTGAGTTGAAACATAAGCATGGTTTTGATTTACTAATTGATCAATATAGTCAGGGATTTTATCGATTTTTTCAGTGATTTGAATATGTTTAATAGGCAGAATATTTAGTTTTTTTAAAAGTGATTTGTAAGCAGTGATTTGTTGTTTTGTAACAACTGATTCGCTTACTTCTTGTTGTTGAGCAATCTTGATGATCTTATCGTCAATATCAGTGATGTTTTGAACAAAATTAACGGTATATTTAGCCTTTTTTAAAACTCTATTTAAAACATCAAAAACAATTAATGGTCTGGTGTTGCCTAAGTGCAAATCGTTATAAACTGTGGGTCCACAGAGATAAATGTTAATGGTTTTTTGAACTAGTGTTGTTGGTTTTTGACTAACACTGTCAACAATCATAAACCTATGTTCAAATTGCTTCATTAATGATAGGAATGTTCAGTTCTTTGGCGTGATTGATTTTTCTTAAAGTTGGTTTGTTTCCTGCAATAACAAAGTCAACTGTTGGTTTGACTTCACTTGCAAACTGGCAATCAAACTTAGCTGATAATAAATCCTTAATTTGGTCACGACTAATGTTAAAGCTACCGGTAATAAGAAAGCGTTTTTGAAAATAAATACTGTTAGTTTCAAAGTTAATTTTCAGTGGTAATTGATCTGTTTTTACTTGTCTTAATTCAAGTTGTTCAATTAACTGCAAATGGTTAGGGTCATGAAACCAGTTATACAATGATTCAGCTACTGTTATTCCTACATCATTTAAACTAATTAAGTTCTCTAAGCTAGCATGCTGTAATGCTTTGATATTTTTAAAATGATTAGCTAAATTCTTAGCTAATACATTACCAACATGCTTAATACCAAGTCCTGTTAGTAATCTAGCCATTCCTTTTTGTTTTGAATTTTCAATGTTATCAACTAACTTGTTGAAAAGTTTATCACCAATCTTCAGATCTAATTTTAAAACTTGGTTTTTCTTGTCTTTTAAATCATAAAGATCAACTATAGATCTAACCAAATTGTGTTCATAAAGTTTGGTAATAGTATTAATATTCAACCCGTTAATGTCCATAGCAGTTTTAGAAACAAAATAGTTAATTAACTGGATGTTTCGCTCCTTACAAGTTTCATTGGTACAATACTGATCAACTTCATCAACTATTTTGACTAGTTTTGAATTACATGAAGGACAATATTTTTGCTCTTGAATTATGATAGTGTCATTTTTTCTTTTTTCAAGATTTACCTTTAGTACTTTAGGGATAATTTCCCCAGCTTTATAGATAACAACAGTGTCATTGATCCTAATGTCTTTAGTTTTAATGTAATCAAAGTTATGTAAAGTAGCAGCTGTTACTTTTGTTCCATCTAAATTAACACTTTCTAATTTAGCAGTATAGTTCACTCTACCAGTTCTACCAATCGTTATAAGAACTGCTGTTAATTTAGTTTGAACAAATTTAGGACTAAATTTAAATGCTATTGCCCAACGTGGTGATTTACTTGTAGCACCTAATTGTTGATAAAAAAGCAAGCTGTTTAGTTTAATAACTAAACCATCAAGATTAAAAGTTAACTGTTCTCGTTTTTTGTCAAACGCTTCCAAGTAATTAATTAATTGAAATTTGTTTTGAAAAACCCTGATGGTATCACTAACTGGGAATTTTCACTGCTTAAGCTGTTCTAAAACCATAGTTTGAGTAGTGATTGACTCTTCCAAACCATTAGGGATGTAATAAAATAATGCCCTTAATTTGCGCTGTGCAGTGATTTCACTGTTTAAATTACGTATTGTACCTGCTGCTAGATTCCTTGCATTAGCAAATGGTTTTTCAAGTTGATTGTTAATTGCTAAAAAAGTTTTTTTATCAACAAAAATCTCACCCCTAATCTCAATTGTTTTTGTGAAAGGGATTGTTAAAGGGATAGATTTAATGGTTTTAACATTATTTAAAACATCTTCCCCAACACTTCCATCTCCTCTGGTTAAAGCATGAACTAAAACACCATTTTTATAAGTTAGAGAGATACTAACTCCATCAATTTTAGGTTCAACTACAAATTCATTTTTTGAGTTTGTTTGAAAGTTAATATTATCAATAAAATTAGCAATTTCTTTAGTTGAAAAAGCATTTTCCAAAGAGAGCATTGGACTGTTATGGTTTAACTTTTTAAAACCCTTCACAGCTTCTCCTCCCACCCTTTGGGTAGGGGAATCAATTTGGATTAAATCAGGATGATCTTTTTCTAATTGTTGGAGTGACTTATACAACATATCATACTCAAAATCATCAATTAAAGGTTCGCTTAAAACATAGTAGTGATAGTCATAGTTTTTTATTAAGTTAACCAGCTGTTGAATCTTCAATTTCACATCCATCACTACTATAAATAAGAAAAATCCTGACTTAGTTGATACCAATCAGGATTTTACAAAGAAGTTTTAATTTGGAGCAGATAACGGGAATCGAACCCGCATCTTAACCTTGGCAAGGTTATGTTCTACCATTGAACTATATCTGCGTTGTTACTTTAATATTTAGTATATTTTAACTTGCATGGAATCTGAAAACCAAATTGCAATCCTGGATTATATTTTTAACCAAGTCAACCAGCCCAATCAACCAAAAATAGTTTGGTTCTCTGGGGAAGGGGAGGATGAGAAAATTAATTTTTTAATCCGCTTAAATGATTTTTTTAAACCAAAATTTGTTGAAAATACTAATGATAGTAGCTTTTTATTAAGTTTTAGAAACCATGTTGAAACTAAAAATTCAACGCCTTTAACCCAAGCTAACTTTGCTAATATTGCCAACAAATTACTAGCGGTTTTATTTGGTTCATTGCAATGAAAACAGTTAAATAAACCAACTGGAAACTGATTTCTTGTAATTTTGTTTTTAGCTTTATTATGGTTAAGACAATGCTGACTAAAACTTCAGTTAACTAAGATAAGTAAATTTGTAAACCAAAAGGGCATTTTGAGTTTTATTAAACAACAATGGCCTATTCTAACAACATTGGTAACAGTAGGGACCACATTAGGTACTCCAGTATTTTCATTAACAATAGCCCAACAAGATGGTATTAAGCAAAATGCGGGGAATGATGTCTTTATCTTTTTGATTATCTTCTCTGTTTTTTCTATTAGTTTAGGGCTTGTTTCATCACTTATCTTTTTAGTCTCCAGCTTATTTTCAATTCGTCAGAAAAAAACGTTGGATGCTTTGGATAAAGTGTTATCAAAATTTATTGATAAATACTTTTTTTTAGATGAAAAAGAGATCAAAAAACAACTTAAATATCAGTTTAAAAACAACGGTGTTTGCTTTTTTTATGGTTTTGATTTTGATCAGGCAGAGTTTCTTGAACAATCAATGAACTTAATGTTGTTGTTAAAGCAAACCAATTGTTTTATTTTAGTTGGTTGTAAAGAAAGTGAAATGACGCTCATTAAAAACAAGATAGAACCTAACATTAACTTAAAACAAAACAGTTTTTATCTGGATTTAAGCAATGAAATTTCACAAGTAGAACAGATCAGCAAATTTAACTTGTTGTTTAGGCAATTGAGGCTCAGTTCTGAACTATTTTACTTAGAAGATTTTTTTGATTATCTAACTACTGCAAAACAGATAGTTAACTTTCTTTTTTAAAACAAAACCTAACCTTGATCAATTTCAAGAAAATCAAAAGAAATTATTTGATTTTTTAGCGTTATGAGCATTAGTAATTGGTACTGATTTTGAGTTTAATAACGTGTTGTGATCATTTAATAACCACTTTGTAATTGATAACAGTTTTAAACAGGAATATGACAAACCCAACATTACTGCTTTCTTTAATCGTTCTTTGCAATTTTTTCAAGAAAATAGCTTGGTTTTAAAACCTGAATTGTTTAGTTTGCAAAAATACACAAAGGATGTTTATGGTTTAAATGTTATCAACCAACTAAACTTAAATAAGCACCCTATGCTAATTCCATTAACATGGGATAAAAAGCAAAAGTTTATTAGCTTTATTGAAAGCTGTGTTCAAAAATACAGTCAAGTTAAAAAAGATAACCAGGTATTTAGTTTAACAGTTGGTAAGCGCGTCTTTTTTCTATTATTAATAAATAAGCAGTTTAAACAAATTAAACTTGAAACAGCACTAAAGTACCTTGGCTTTAAAACTTCCCTTGGTGCAATGGACAGCACAACTGAGTCCTAATCAGTAAATAGAGGTTCAACTCCTCTAGGGAAGGCCAATTTATGATAAAAACTTAGTTAAATTGCTAGGTTTTTATCTAGTTTTAGGGGGTGTAGTTTAGTGGTAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCATTTACAGATGCACTTCAATTCAAACTTTAAAGAATGCTTTAATAAGATTGCAAAAAAAGTTAATTCACTGGACAGTGAATACTATGAGTTTTCTTCTTTTATTGAAAGGATTAGAACTACCTTTGGACTTTTAATTGCTTTAACTGTTTTAAGTAACCTAATCATTATTAGCTTTGTTTTAATTTGATTTTTTACTGATGGCTTTGGTCAGTTAAGGCTGTTATTCTTTACGCTTTTTATCCCCTTTTTTATCTCTTTATTAGTAGCAATCTTTCTAATCTTTCTTAACAATTCATTCCGTAATTTTTTTCAGATTAATGAAAAGAACTGGCTGTTCTTATGGACCTGTGTTTTTTCCTCATTACCAATCTTTAACTTGTGGTTAATTGTGAGATTAAATAAAACTATTAAGAATTTCGCTAGTGATTATGGTTTTAAGATTGTTAATAAATATAACAGTTTAACAAGCGGAATTTTTGTGTTTGACTTTGCTGATTATGTTAGTTTTGAAGCCAACCTGACTAATTGAAAAAACACAAACGATAAAAATCGTAATTTTGTTAACTTCTTTGAAACAATAAGCAAAGAAAAAACTGGTGTTGTTCAAAAACCAGTTCTTAACTTTCAAAGATTATATGTTAATCGTTTGTACTATCAAAGTAAGCTAAGTGTTGGTAGCAACCAACAGACTCCCCAAACTGCTTTTGATAACCTAAGAAACTATGTTGAAAACAAACAGCGTGAAACAGTAAGGGTTAAGCAGTATATCCTCACTTAATTAAGTGAATATATTAATATTTATTGGTCTTTGTCAAAAAATAAGATGAAAAAGGCAATCCACTTTCAGAGTCAACCAGTTGTTTTTAACTGTGCTTCATGCAATAGCAACTTTACCATTGACTCCACTGCCAAACAAAAGGATCTTGCCATTGACATTTGTGGAAAATGTCATCCTTTTTACATAGGGCAATTAACCAAACAAACCGTGCATGGACGGGCTGAAAAACTTTCTCAAAAGTTCAACGCTGGAAAGGCTTTTTTAGAAAATAAAACTAAAAAGAGTAACCAAGCTAAAGTTGAAAAACAAACTAGGCACCGTTCTATTAACGAGCTTTAGTGGATTTTGACAAACAACTCTTTTTCAATGTTGAAAAGATTGTTGAACTTACTGAACAACTTGAAAAAGATCTCAATAAACCTAACCTTAGCTTTGAGCAGATTAAAGTTATTAACAAAGAGTTAAAACATAAACAACCTTTAATAGTTAAATTCAAGGAGTTGCAAAAGCTGGTTGAAAATGCTAATGAAGCTGAACAAATTCTTAACAATTCCAGCTTAAAAGAATTGCATGAAGAAGCTAAAAAAGAACTTGAAAAAATCAAAGCTAGTTTACCTAGTTTAGAAGAGGAAATTAAGTTCCTTTTACTACCGGTAGATGAAAATAACCAAAAGAATGTTATTGTAGAGATCCGTCCAGCTGCTGGTGGGGATGAATCTTGCATCTTCTTAAGTGATCTTTTTAATATGTACAAAAACTATTGTACTAGTAAAAATTGAACGGTTGAACTTAATGAAATTATCCCTGCAAGTGTGGGGATTAACTTTGTTTCTTTTGCGGTAAATGGTACTGATGTTTTTGCTAAACTCAAGTTTGAATCAGGAGTACATAGGGTGCAACGTGTTCCTTTAACAGAAGCTAAAGGTAGAGTGCATACCTCAACAGTTACTGTTGCTGTTTTACCTCAATTAGAAGAGGTAGAGATCACCATTAATCCTAGTGATTTGAGAATCGATACTTACCGTGCTTCAGGAGCGGGTGGACAACATGTTAACAGAACTGAAAGTGCAGTTAGAATTACCCATCTACCTACGGGAATTGTTGTGGCTTGCCAAGAGGGTAAATCCCAGTTTTCTAACCGTGATAAAGCAATGAAAATGCTACGTGCTAAGTTATGGGAAAATGCTCAAAATAAACAACTCTCAACCCAAGCGGATTTAAGAAAAAGTCAAGTTGGTAGTGGAGAGAGAGCTGAAAAAATTCGTACCTACAACTATCCTCAAAACAGAATTACAGACCACAGGATTAAATTAACTATTAATAAGCTTAATACTGTTATTTTGGGAGATCTTGATGAGATCATTGAAGCTTTACAAGCTGATGAAAAGAAACAACAGTTGGAAAAGTTTATTAGTTAGATGACTCTGTATGAGTTTTTTTTAAATCAAAAGTTAGTTTACCAATCCAGTCCCCATTTTAACGGGGTATTTTTAACAATATTGGAACACTATGGTTTTCAATTTAAAACAATTGATAAACTCTGAAAAAGTAAGCTTCTAATTACTAGTGAGTTAACTGATAAAATCAAACAACAATTAAAGTGTTATTTTATTGAAAAGATCCCTTTGCCCTATTTGTTGGGAACAATTCAACTAAGGAAGCTTACTTTTAAAACTAAGAAAGGAGTTTTTATTCCTCGAATTGATAGCTTAGCACTAATTGCAAGTGTTAACTTAAAAAAAATAAAAACTGCACTTGACCTTTGTTGTGGTTCAGGTACTTTAGCCATTGCTTTAAAAAAGAAGTGTGATACACTTGATGTTTATGGTAGTGATATTGATATCCAAGCATTAAAACTAGCGCAACAAAATGCATTAATTAATAACGTTAGTATTAATTGAATTGAAGCAGATTGATTTGATTGTTTTAACAAGATAAAAACTCCGATTGATTTAATTGTTACAAACCCACCTTATCTGAAAAAAACACAACTAAATAAAACATTAAATTATGAGCCTAAGCACAGCTTGGTTTTTCAAAATAAAAATAGTTATTTTGCATACAAGCAGTTGTTTAATCTATTACTAACAAAACGATCAATTAAACAGTTAATTTTTGAATGTTCTTTATTTCAAAAAGAAAGGCTATTAAATTTGTTTTCAATCTTTAAATCAAGGCCGATTTTTAACTTTCAAAAACAGTTTATTGGTATGAAAGTTGATAATCAAAAACTCCCAGTAGTTGATATTAAAAATACCAAAACTATTAAGCAACTTTTAAAAATGGGGCTAGCAGGAATTGTAAATACTGATACACAAATGGGATTAATTAGTTATTCAGAGTCTACTCTTGACAAAATTAAACAACGTGCACTTAACAAACATTATGTATCAATGTTTGGGTTAGAAGAATTAAAGAAGTTACCAAAAAAACTACAACAAATTGCTAGTTACTTTTGACCAGGTAGTTATACCTTTATTAAAAATAACAAGAGCTACAGGGTTCCTAAAAACTTGGGCTTATTAAACCTTTTTAATGCAATTGGTAGGGTTTTTTGTACTAGTGCTAATATCAGTAATCAAAAACCATACACCAAATTAAGTGATTATCAAAACGATAGTTACTGAATAAAGCAACCTTGTTTTATTATTAGAAGCACTTCTAAAGTGCAATCAAATAACACACCTTCACTTGTCTATAATTTAGATACAAAACAGTTGGTTCGCACCACAGCTAAACAAACAAAACAGTTTCATAAATTAATAACTAAACACCAGTTAGCTATCTAATACACAAACCAAAATGAAACCAAAGATAATAACAAGCTTTTCAATATAAAAATGCTTAATTCAGTTTTATTTTTGAAATAAAACTATTTTTTTTAATTAGTATATAGCATCAACTACTAAAACATAAGGGATGAAGCTCAAAGGTTTTTTAGCTGTTGGTGTTAGTGTTTTTGGTTTTTCTGGTTTACTGATGGCTTGTAGTGTTGTAAGTCAGTTTGATCAAGTGGATGATGGCAAAATTAAGCTAGCATCTTCATTAACTTCGAAACGCGCAGCGGAAGCTTTAGAGACAGTAGTTAAAAAATATAACGATACTAAAGATCCTGGTGATTATCCAATTGAAATAGTACAAATTGCTGGGGGTTATGATGGGGGTAAAAAGGATGTTCAAACCAAGGTTAGTACCAAAGATAAAAATAACTTTTATAACCTGATTTTAAACTATCCAGAAATAGTAAGTACCTTATCAAGGTCTAAAATGGCTTTGAATTTTGATGGGGTTAATGTTGATAAACTCCATCCTAACTTTTTAAGCTTTAACAGCAGAATAGGTGGAATTAGAGATGATGGAATCTATGCTATTCCAATATCTATGTCCACTGATCTGATGGTCATTAATGGCCCTGTTTTACACTATATTCTAAACAGTGCTAGAAAAGAAGGTACACCAACTAGCACTACTGTTCAAGCAACTGTCAGTTCAAGAAGTGCAGAAAAAAAAGGTACATTAGAAATTGCAAATGATAGTGAAACTACTAAACTTTGACAGAACATCCAAACCACTGCTCAAAACAACAGTAATGAAACAACTAAGGAGCAAAAACAAGTAAAAAGATCTAGTAGTTCTTCATCTACAACATCTACTACTGGTGAAACTAAAGATACTACAAAATCAGATAACAAGATTAAAGAGTTTTGGGGTGAATATCAAGAAGTGGATGGAGGGTTAAAGAATTTTACCTTTAAAGCAAGCATCTTTGAAAACTGAAATGAAACGTTAGATTTTGCTACTAGAATAGCAAACTCTTTTCCTGAAAAGGTTAAAAATATAACAAATAAAACTGGGCTTGATTTACAAGGTGTTTTAGGAGTTGATAGTAGTTCTAATGCACTTTATGCAGCAGTTTTTGCAGCTGGTCAAGCTAACTATGATAACTTCTTTTTTAACATCGATAAAAGAACTGGTTATGCAGATTACTCTAACTTTTTAAATAAAGATAGTTCATACCAAAATTTAGAGAGTGTTTACAATGACTTTTATAAATTAATCCAAGCTAATGGTTTGTTTGTTAACCGTGGTGGTTCCTATTCATCCAACTTTGAAAAATTTCACCAATTGGCATTCTCAGTATCTTCTTCTGGAGGATACAGTTATTACTTTGCTAAAGATAATGCTAAGCGCTTAAAGTTTAGTAATTATGCTATTGAATATCCTAGTTTTACCCAAACAATTCAAGCTCCTAATTCTTCAGAAACAGAAAGTAATTTACTTGGTACTTTTAAATTAAGTGAAAAAGATATCAATCTATATAAAGGTTCAATTCCTAGTGGAAAACAACAAGGAGTTGATGCTATCTTAATTAGTAACCCAAACTTAATTAATATTCTTGAACAAGCAAAACAAAAAAACACTGCACAAGGAAGTGAATCAACCACTAACAAGATAATAGGTTATACCACCACTGCAAATGTTAATGTTGATAATCAAAACATCTTTTCTGTTAGCAAACTTAACAACGAACAGTTTCAAAGAAAAATCATTGTTAATGCCACTGAAGAAACACTTGATCAATCCCAAACCTTACAGAGCAATGAATCAATTGTTTTACCAATGCCTGGTAAATACAAATCAACTGATAAAAATAAAGTAATGATCACCCAAGGTCCTAACTTAATAGGCATCCATGCAAATGAAAAAGAAAATATTGAAACTAAAAAATTTGTTAATTGGTTTTTAAATCAAAGTATTACAGATTGGAATAGTAATAATCAGCAAAAAAATAGTGATCAAACAACAAAAACTGCTGCTGAATATTTCACTGATCAAGCTTCTTACATCCTTCCTTTAAAGGAAAAATTTAACAAAAGTTCAGATTTAGAATTGAAAGGCAGTAGTAGTTCTTCTAATTTAACAACCAGTAGTGCTAGCGCCTCTTTGTTAATAAGTAATAATAGTTCAACTGCAAGTTCTCCTGCTCCTAAAAAAACAAACAATAATTCTAATACCTTTACAGCTAAAGCACTAGAATTATTCCAACAAGCTGCTAACAATGAAATTATTCCCTTTAGTGATCCAAGTGACTTTAGGAACGGCACATTCCGTAATAATATAAGCAGTAGTTTTAATGCTGCGGTGAATTCTAAGGTTAGTTTTAATCAATTTGTCCAAAACTTTATTAATAGTTTAGGATCTGGATTTAGAAGATAATTAGTTAACCTCCCCCAACCTCTATTTTCTGTTACTAGTGCCTAAGGTGGCATTAGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCTTTTGATCCCACCCAAGGGTTGCAAAAAGATAGTCCAGTGAAGGATTCAAGTAAACAAGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTATGGCTACCTCTACAAGAGATAAAGCCCTCAAGATTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAGAACAGTAGTGGGACAGAGGTGAAGTTGGATTCACAGAAGGATTTTCCCCAAGGAAAGGTTTGAAAACCGGTGTTGAAAACAGATGAGATAGAAAAAAATAGGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAATGGTAAACCCTTCTCCAACTCCCCCTCCCCTTCAACTTCCGCTTCCTCAACCCCACTCCCCACTTTTTCTAACATTGGCGTAGGGGTTAAATCAATGATCACTCAACACTTAAATCAGCAAAACACCCGGTGGGTGTTTACACCTGGTAGTACACCAGACATTTGAACAGGAGCAGGTTATAGAAAAGCTAATAACAACAATAACGGCATCCCTTTTGAACAGGTGAAACCTAGTAGTAGTAGCAACACGTTTAATCCCAATTCAGATGATAATAAAGTCACACCATCAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACCAATAAGAATAACCCCCAGCGCAATCAGTTGTTGTTAAGAGCGTTATTAGGAACTATTCCGGTCTTGATCAATAAGAGTGGAACGGGAGATCAATTTAACAAGGATAGTGAGCAAAAATGAAACGAAACAGATAAATTAGGAGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGTGCTTCTTATAAGATTTTTACTTATTTAATAATTAAAAAAAGTGTTAGGTTTTTTTAGTTTTTTATCTATTTAATATTTAAGAAATTCTCAAATTTTTCTTAGTTTTTTATTTGTTTAATAGTTAAAAAAAGCGTTATGTTTTATCTATTTTATTAGTTAAAAAAGTTTTGAATTTTTATCTATTTTTAGTTAATAAAAGTCTTATGTTTTTATCAAATTTTTATCTGTTTTTTGGTTAAAAAAGTTTTAGATTTTCTTTCTTAAATTTATTTATTAAATAGTTAATAAAAGTGTTAAGTTTTATCTATTTTTAATTAATAAAACCTCGACCCCTCTTCCTATCAAATCGCTGACCAAACCATCCATAACACCAACCTGTTTGTGTTGTTCAAGTCTAGGGATGTGAAGCTTACATATAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAGTCAAGGTGAAAAACCCTCCTATGTGGTCGAGTTTACTAATTCCACCAACATTGGCATCAAGTGAAGGGTAGTGAAAAAGTATCAGTTAGATGTACCGAATGTTACCAATGAGATGAACGATGTACTGAAAGAATTGATCCTAGAACAACCCCTTACCAAGTATACCTTAAACAGTAGTTTGGCCAAAGAAAAGGGCAAAACCCAAAGGGAGGTGCATCTGGGTAGTGGGCAAGCAACTAATTGACGATCGATGCGTAACTCCATTGGTCTGAATGACAATCCCAGCCCCAATGCTTCAACTGGGTTTAAATTAGACAAAGGCAATGCATATAGAAAACTAAGTGAATCCTGACCAATTTATCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAATGGGTGGAGTTCAACTGAAGCAACAATGGCAGCAGGGGATGCGCCCCTAAGTACAGGAGGGAGATCATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGAAAGGATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTACCCAACTCTACCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAGGTTAATTGAGCCTGACAATGCAACAAGTGCAGCGAGCAGCATGACCAGCTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAACAGAAGTTGGGGAAGGGGGGAACAGCAATGCAGGGAAATAATGGTGGAGGGGGTGTTAGTCAAACGATTAACACCATTACCACTACGGGAAATATTAGTGGCAATGGAACCATTCAAACGGCTTATCCGGTGAAAAAAGATGAAGCTTCAAATGTAGCGATCAATTCCTTGATTAACGCTACGCCCTTGAATAGTTATGGGGATTTAAATAATGCTAGTTTTTCTAAATAATTAAATTGTTAATAACAAAAAAATCTCTATTAAAAAAACCAACTTTAAAGTTGGTTTGAAATTCTAAATGGCGCGCCCAATAGGACTTGAACCCATAACCTTCTGGTCCGAAGCCAGACACTCTATCCGATTGAGCTATGGGCGCATATATTAATAAATTTTATTAATATAAGCAAGAACAATCTAATTCTTTATTAGAATTTAAACGATTTCATCTAATAGTTCAAATCACATATGGCAAAAGATAAAAAAAATAAGGTTGATGGAACAGAGCAATCAGTTGATCTATTTGAACGTACAAAACTTGAAGATACACAAGTTTTAAATGAAGTTGAACTTGATGATATTAAAAAGATAACAGAGCTTAGAAAAGAACTTGAACATACTTTTGAACCACAAACAAGAATGCAAATTAAGCGTGAAATTAAAGAAATAGAACGCAAAATGAAACGTTCTAGTCGCTAATTGATGTTTGTTAATTTACATACAAATTCATACTATAACTTTCTCAATTCTGCCCTTTCTCCTAAAAAGCTAGTTAATCTAGCAATTAATGATCAGCAAAAAGCTGTTGCTATTACAGATCCTAATCTTTTTGGCGCTGTTGAATTTTTTATAACTTGTAAGCAAAATAATATTAAACCAATTATTGGTTTAAACTTAACTGTTGAATACCAAAAAAATGATGTTAAGTTATTACTAATTGCTAAATCAAATAAAGGCTTTCAAACGTTGAACAAAATAGCATTAATTCAACAAAAACTTGAAATTAATTCTTTAGTTGATCAACTAACAGATATTGCAGTAATTATCTGTTCTTTAACAACATGAAAATCTACTTATAAGGATGTTTATCAAGCAAAAGGAATTGAAATAAATCAAACCCCGATTGCCATTCTTGCAAATGCTGTTAACTGTGAAAAAACTAATAGCGATCAAGTAGTTTTAACAGTTTTGAAACAAATGAAACAAAACCAAACGGGAAAAATAACTACATTTGATTGGGATCTTAAACAAAAATTAAATCAAATTTCAATTAATGAAAATTTAAAAGTAAAGAGTGAAATTCAACCTTTTTTAGATCAAAAAACTGCACAACAATTATTCAGTGAAACAGAACTTAATAATCTGAATGATCTAGTTAATAGATGTGAATTAGATTTGGAGCACCTAAAAGCTGCTTCACTTTCTTTAACTGATAATGATGCAGCAGTTTTAGAAAGTTTGTGCCAAACCAATTTAAAACAGTTTTTAGATAAAAATCAAGATCTAAATAAAAAAGCCTATCAGCTACGTTTAGAGAAGGAATTAAATGTTATCAATAAACTTAATTTTGCTAGCTATTTTTTAGTTGTCAATGATCTTGTTAATTATGCTTTTAAAAAGGACATCTTAATTGGTTCTGGTAGAGGTTCTGCAGTAGGATCATTAGTGGCTTTTTTATTAAACATTACCAAGATAGACCCAGTCCAACACCAGCTTATTTTCGAACGTTTTATCTCAACCCACCGTCAAGATCTACCTGATATTGATATTGATATCATGGAGAATAAAAGAGCAGAAATGATAAATTATCTGTTTGAAAAATATGGCAAAGAAAACTGTGCACAAATTGTTACTTTTCAACGTTTTAAAACCCGTTCTGCTGTTAAAGAAGTTGCTAAATTATTTAATGATTATGGCATTAGTGACATGATCCTAGGAGTGTTACCTAAAGATCAAACTATAACATTCACTGATCTTAAAGCTACTGAAGATAGTGCTTTACAACTTTGTTTACAACAGTTTGGTTTAATTGTTGAATTAGCACTAGCAATAGTTGATTTTCCAAGACAATCAAGTATCCATGCTTCAGGCATAGTTATCGCTTCAAATTCTTTGATTAAAACCATTCCCTTGTTACAGCTTGACAATAATCACTTTTTAACTCAAGTTTCAATGGAATGGTTAAGTTTTTTTAATCTCAATAAGTTTGATCTGCTTGGTTTAATTAACCTTACTATGATTAGCGATGTAATTACCCAAATTAAACCATCTAACCAGACCGTTAACCAGTTTTTAAATACCATTTCTTGAACTGATCAAAACACCTTTATAAACTTAGTAAATGAAGATACACTAGGAATCTTTCAACTTGAATCGTTTGGCATGAAAAAATTACTGGTTCAGATTAAACCTAAAACCATTAATCAACTAGCAATTGTTCTAGCGCTTTACAGACCAGGTGCACAGGATAACATTAACCTTTTTATTAACCGCTTGCACAATGGTTATGATCAATCTGACATTGATCCTAGGATTTTACCCATTGTGAAAAATACCTATGGAGTTTTAATTTTTCAAGAGCAGATCATTAACATCGTTAAAGTTGTGGCTAACTACTCTTTAGAAGAAGCAGATAGCTTCCGTAGAGCCATTTCTAAAAAGGATGTTAAATTGATCCAAAAAAATAAGCGTAACTTCTTTGAAAGAGCAGTTCAAAATAACTTTGATTTAAAGACTACTACCAAAATTTTTAGCTACATAGAACGCTTTGCTAACTATGGGTTTAACCTTTCTCATGCGTTGGGTTATGCACTGCTTTCATACTGAACAGCTTGACTTAAAACTAACTATCCTGTTTATTTTTATTTATGGTTATTAAACCATTTTCAATCTAGTAAAGACAAACAAAAACTAATTATTAGAACTTTAGAAAAAAGTGGTATTGAAATTTATCCACCTCTTTTAAATAAAGCTCAACCAAATAGTGTTATAGAAAATAAAAAAATTTATTTAGGTCTAAACCTAATTAAGGGAATTAATGACAGGTACATCCAAAACTTACAAAAAGTGCAACATTTAATTCAAACTCAAAATAACTTACAACTAACTGATGTAGTAAGTTGGTGTTTGGATAAAACCATTGGTGATATCCCTTTAAAAGATTTACTTTTATTAAAAACTATGGGCTGTTTTGATTTTTTTGAATACACTTATGACTTTAATGATGCAAAGGATTTTTGAATTAAAAGCGATCACCTATTGTTTACCAGAATGCCTTTAGAAAAAAAGGATAGTAATTTTTGAATTAAACAATTTTTTACCAATTAGTTGAACATGAAAAAAGCAATCCTGATTGATGGCAATTCCTTAGCATACCGTGCTTATTTTGCAACGTGAAAACAAGTTGAATATGCTAAGCAAAATAATTTACCTTTCAATAATGCAATAAGAACAATGTTACTAATGTGTTGGAATTTAATTAAAGCCAATGTTTATCAATATGGAATTGTAAGTTTTGACACTAAAGCGCCAACTTTCCGTGATCAAATCTATGAAGGATATAAACAAAAAAGGGTTAAAACTCCAGTTGAACTTTTAGTACAAATTCCTCTAATTAAACAAGCGCTTGTTTATTTAGGGTTTTTAGTTTGTGAAAAAGATGGCTTTGAAGCAGATGATTTGATTGGTAGTTATGCCAATTTATTTACAAAGCAAGAAATAACAGTTGATATTTACAGTTCAGATCGAGATATGTTGCAATTAGTAAACGCCTTTACTAATGTGTTTCTCTGTATTAAAGGTACAAAGGAGATGGTTATGTACAACAATGAAAATTTCAAATCACTTTTTTATGGTTTGGCGCCCTATCAAGTTGTTGAATATAAGGGGTTAGTTGGTGATAACAGCGATAATTTAGCAGGGATTAAAGGGATAGGTCCCATCAAAGGGATAGAATTACTCCAACAATATGGAACCATTGATAACATCTACACTAACTTCAATAATCTCCCCAACCAACTTCAAAAACTTTTAAATAACCAAAAGGAAATAGCTAAAACCTTTAGTTTTCTAGCTAAAATTAAAACTGATATTGAACTTGATCAAAACATAGATCTTACTGGTTTAAAACCAATCCAAAAACAAGCGTTAATTCAACTTCTAAGTGAAAACAAAATTAATACTTTAGTTGAAAAATTTTCAAAAATATAATGCCTGAACTTCCTGAAGTAACTACTGTTATTAATGAACTTAAAGAAACTGTTTTAAATAAACCTTTAGATCAAGTTCAAGTTAACCTAAGAAAGGTTTTGAAAAATATTGATCCTCAATTGCTGAATAAACAATTAAAAAATCAGTTTTTTACTGATATTAAGCGTAAGGGTAAATATATCATTTTTCTTTTAAGTAATGGTTTGTATTTAGTTTCGCATTTACGTATGGAAGGTAAATACTTTTTTGAAGAAAGAGGTAGTAAATTTAATCAAAAGCATGTTTTAGTAGAATTTCATTTTGATGATGGTAGTCAACTCAATTATCATGACACCAGACAATTTGGAACGTTCCATTTGTATGAAAAGTTAGAACAAGCAGCACAATTAAATAAACTTGCATTTGATCCTCTAGAAGCTGGTTTTGACTATAGGAAAATCTTCCAAAAAGCACAAAATTCAAAACGTAAAGTTAAAACTTTTATTTTAGACCAAACAGTGATTAGTGGAATTGGCAATATTTATGCAGATGAAATCTTATTTGCAAGCAAAATTAATCCTGAAACAATGGTTGATCAACTAACAATTAAAGAGATAGAGATTTTATGTAAAAATGCTACCAAAATTTTAGCTAAAGCAATAGTTATGAAAGGTACTACCATCAGCAGCTTTAGTTTTAAAAAAGATCATACTGGAGGCTATCAAAACTTTTTAAAAGTTCACACTAAAAAAGATCAACCTTGCTCAGTTTGTAACCAATTAATTGTTAAAAAGAAGATTAATGGAAGGGGGAGCTATTTTTGTTTAAACTGTCAAAAAATCACAACCAAAGTTTCTACAAAACTCAATCCATAAATTTTTTAAAACTTTAAATATCTTAAAATTAACTTTTAACAAACTAATTGAAAACTTTATTTAACAAAGTAATGCTGTAATTAATCAATTAGTTAATGAACATTAAATAACATTTATGAAAAACGAAATTAAATATCTTTATTCTGACTTGGATGGTACTATTGTTAGCTGAAATCCTAAAACAGAATTTGTTTATCAAAATAAAAGTTATAAAAATTTCCATGAAGTTAGTGATGCTACTATTAGTGCTTTTTACCGATTGCAACAAAAGGGAATTAAGGTTGGTATTGTTACTGGTAGGGATTATTGTCGGGTGTTATGACTTGAAAAACAACTTAGAACAGGATTGCCTACCATTACTTTAGATGGGGCTATTATCTTTTATCAAAACGAAATCTTAAGTCAAACTTATTTAGATGATAGATTTATTGAAGGGATTAATAACATAGTAAAGCGCTTTCCTGAAGCTGCTTATAAACTTAACAGTGGTTGAATTAGTTACTTTACTAAAAACCCTTCTGTTATCTTTGAAATTGATTATGCTTTTCTTGGCTATTTCAACCCTAACACCAAACTACAAAAAAAGTTTATAGACAGTACTGAAAATTGAGATCTTAATAAACTAAAAGTTAATCAGGTTTACTTTGATATTGATACTTGCCCCTTAGCAATGCAAAAGGAAATAATTGAACTAATTAGTGTTAGTGATGTAAATGCCAAAATCTATGAGCACTCTATGTACATTATTAAAAATGGTGTTTCTAAAGCTAGTGCATTGCAAAGCCTTAACCAGTTTGCAATTCCAATAACAAAAGATAACACTATTGTTTGTGGGGATGGAGATAATGACATTGAAATGATGCAGTGAGCTAAACACAGTGTCTCACTAATCGGTAGTAATCCCAAATGCTTTGCTCTAGCAAAATACCACACTGATAGTGTTGACAATGATGGTATTGCTAACTGGATTGAAAAAAACTTGTTATGTTAATTGCAATCGTAGGTAAACCAGGTGTTGGTAAAACCAGTCTATTGCAATATCTCAAAGATAACTATCACTTTTCAGTTTTTTATGCAGATAGTTTTATCCATGAACAGTACCAAAAAAACAATCCAGGTTATCAATTAATCATGGATCATTTTGGCAAAGAGTTTGTCAATCAAACTGAAGTTGATCGTAAAAAACTAGCAAACTATGTTTTTAGTGATGATAAGTTAATCGAAAAACTTTCACTAGTAACAAAACCGCTGTTAATAGCGTGAATCAAATCCTTAAAAACCCAGTTTCAAAAAAAGCTAGCACTAATAGAGATTGCTGTGATGCTTAACTATTGAAATGAATATAGATCGTTGTTTGATTATGTGATTAAATTAGAAAGGGATGATCAGCTAGTTAACTTAGCTTTACAACAACGTAATAGTCATAAAAAAGTTAAGGATTTGATTAAAGAGCCTAATTGCAAAATAGATACAATTTTCAACAACGATTCGATTGCAACAGCTGCTTTAAAGCTAATTAAGTTGCTAGAAACTTTTTTAGAAAGAAATAAATGCCGTTGTGATTGTTGTCATATTCAGTAATAAACTTAGCATTTTTTTTAGCTAAATCAGTGCCATTAACCAAAGCAACTGGCCATTTAACACTTTGAAATATTTCAACATCATTATCAGCATCACCAAAGACCATAGTCTTTTCAGGATCAAGATTATAATTATCAACTAATACTTTCAATCCATAAGCCTTATTAACATCTTTTTGCATGATATCAAGAGCAAATGTCATTGAACTCACATAGTTAATATCTTGAATTTGATCTAGTTGTTTTGCTAGAAAAGGAACTTTTTCTCTGTTTTTAGTTACCAGTAAGATCTTTGTAATAGTATGTTGCTTAAAATCAAAATCCAAAGCTTTTTCAAACTTGTTAACTATGTAGTTATTTTCAATGAGATCAATTCCTACTATTTGACTTCTAGTTGAAAGAATTTTAGCTGAATCAAGCTCATGTGTAAAAGCAAAAACCTTTTTATCTGTATAGAAATAAAATGTTTCTTGAAATTCCAACAACATTTGCATAATTGCAGGTATTACTTTATTATCAATAGGTTTGATATGAACTGGTTTTTCACTAGCAAAATCATAAACAAGTGCACCATTGGAAGAAATTACAGGTAGATTTGGTGTAATAAAGGTAGCTGTGTTTCTAATTAAAATATGGCTTCTACCACTAGCAAAAGTGATTCTAATCCCTTTTTTCTGTAAATCCTTTAAAAACTCAACTGTTTGTTCTAATGGAATTTGGTTGCTTGAAAGCAAGGTACCATCAAGGTCAAAAATAATGTTTTTCAGTTCCATTTAGCTATATACTTTTATCTATTTTTTTAAAAGAACATTAATAATTCGATCCTGAACATAAACTACCCTCTCAATCGGTTGGTTGAGAATGTCATTTATCTCTTTATCCTGTTTAAATGATTCTAAAACATCTATCTCTAAACTACCTTTAGTAAATTCTTTAGCTGCTTTAAATTTGCCATTAATTGAAAGAATAACCTTAGTTTTAGCAGTCTCAAAAAGTTGATAATCAACTAAAGAAATCGCTTGTTTAACAACAAATGGTTCAAGTCCACATTTTTCATTCAATTCTTCAGCAAGAAAGGGCGCAAAAAACGACAGTACTGTTAAAAATCCCTTTGCATAATTTAAGCTAATTTTTTTGGTTTTATAGAGAAAGTTTAAAAAGATCATCATTTCACTAATCACCAGATTTAGTTCATGTTTGTCAAGATGACAATAACTGTTTTTTAAAAACAAATTGTAAGCAAAGATTGTCTCTTGACTAACTTGATCAGTAACAACAGCATGATTAAAAAAGAAGTTATAAACTCGATCCAATCACCTTCTCATCCCGTTTAACCCTTCATCATTTCAAGTTAAACTAGCACTAATTGGGCCCATAAACATTAAGTACAACCTTAAAGCATCTGCTCCATGTGAATCAACAAGTGGTGTGGGGTTAATGGTATTACCTTTGGATTTGGACATCTTTTTACCATCAGGACCTAACACCATACCCTGATTAATTAATTTTTGAAATGGTTCTTTTGTTGATACTAGCTTCTTGTCAAACAAAAATTTGTGTCAAAAACGGGCATACAAAAGGTGTAAAACTGCATGTTCCGCACCCCCAACATAAAGATCAACTGGAAGGTATTGATCAAATAATTTCTTCGCTTCTTTTGAATCAATTGGTCAAAAATTAGGGTTTTTAATCAACATTAAATAACCCAGATAATACCAACAAGAACCAGCTCATTGGGGCATGGTATTAGTTTCCCTTTGGTAATGGATGTTATCTTTGACTATGTTTACCCAAGCTTGATTTCTCATTAGTGGAGAATTACCACTTCCATCTGGTTTGTAATTCTCAAGTAAGGGTAATTCAACAGGGAGTTGTTCTACCAAATGAGGAGTGTTATTTTCATCAAAAATAATTGGAAAAGGTTCGCCCCAATAACGCTGTCTACTGAAGATCCAATCACGCAGTTTATATACAGTTGTTAATTTCGCTTTATTTTGTGAAATTAACTCATTAGTAATAGCTACTTGTGCTTCTTTAGTTGTTAATCCGTTATATGCAAATGAATTTTGCAGCCGTTCTTTTTTATCAATGACGTTGATAATTTTCAACTTTTGTTTACGTGCGAAGAAGTTATCATTTTCATTGTGTGCTCCAACACCCATAATAGCATCTGTTCCATATCCTTCAATTACATAGTTAGCAACATAGACAGGGATCAATTCATTTGTAAGAGGGTGAATAGCATTTGTTAATAAATCTATCCCATCATATAAAGTTGCTTTTTGCTTTAAAGTTGTGGTTTTCTGGAGTTGTTTTTTTAAAAAACTAGCTACTTTTTTATTTGTTTCTGCTATCTTTTTTGCTAACCAATGGTTGGTTGAAACTGCAAGAAAACTAACCCCAAAAATTGTTTGTGGTTTAGTTGTAAAAATTGCAATAGCTTCCTTATGATCTTTTAGTTGAAAGTTAATAGTAACACCTTTACTTTTACCAATTCAGTTCCGTTGCATCTCTTTAATTGGTTCAGGTCAATCAAGTGTATCTAAGCCTTCAAGAAGTGCATCAGCAAAAGTAGTGATTTTCAAAACTCACTGTTTCATCTTGCGTTTTTCAACTGAAAATGAACCCCTTTCACTAACTGCGTTGCCATTACTATCAATTAAAACTTCTTCATTAGCCAATACAGTACCTAGCTGTTCACACCAATTAACATCAATATCAACTAATTCCGCTAGGTTTGCTTTAAACAGCTCACTGAAGATCCATTGTGTGTATTTGTAATAACGTGGATCAGTTGTTTTGAGACTTAAATGATAGTCATAATCAAAACCAAAACTAGTTAATTGATTAATAAAGTTATTAATGTTTTGATCTGTTCAACTGCCAGGGTTTTGATTAGAGTTAATAGCATACTGTTCAGCAGGTAAACCAAAAGCATCAAAACCAATCGGATGGATCACATTAAATCCTTTAGCTTTGTAATACCTACTTATTACATCAGTGATAGTATAAGCTCTAACATGTCCTAAATGTAATCCTGCTGCTGAGGGATAAGGGAACATGTCAAGGACATAATATTTCTTTTTGTTACTATCGCTTTCAAAGCGGTTAACATCTTTGTTTTTTCATTTTTTTAACCACTTTTCTTCAATTAAATTGTGATTGTACATCTAATTGCTTTGATAAGAACGCTTGATTGCATATTTAGTTAAGCGATAGAGTATGTCAACAATAATCGCAATAATCAAACCAAATAACAACCCAAAGCCAACAGGGATAAAAAAGTGAACCATTGTTTTAAATAAGACTGGTTGGTTTGCAACTGTGGCATTAAACCAACTGTTAATGTTGGGAAAAATCGTTAAAATAATCCCACTAGCATCACTGGAAGCGAGTAAATCAGCCCACTGAGCTGTTTGGTATGTATTAAGTCAAAAACTACCAATAAAAATAAAAAACCCAATTACCATCAAAAAAACTAGGATTGCAATTTTCACTAGTTTAAACAAAAGTGTCATTGTAGTTTTCAGTAAGTTATTTCAATTATAAAGTTAGATAAATTTTCAATCTAACTTTTGTAACTTTAATTTTTCAAGGATCATTTTAATAATAGTTACTATTGGTAGTTTTGCATCTATAATACAGTAATTAATCCCAAAATCATTACAAACCGCTTCCATAAATCCAGTGTAAACCCTGTGTAAATTTTGAAAGTAAAGTTGATTTTTAGTAAAGTTATCAATCTCTACTTTGCGGTTACGCATAAATAGTCTTTTTTCAAATAATTTTCAATCCCCATCCAAAATGACATATAAATTAGGAACCCCATGCTTATTAACTAGTTCTTTTGCTAATCTATTTCACAGTTGGTTATAGTATGAAAAGACTGCAGGACGAATGATGTTGTGCTTAGCAAACAACCAGTCTTCAAAGATAGAACGATCAAAAATGGTTGGATTGATAGTGTTGCAATTGTTCTGGTATTTACCAAAGCGATTAAGCGTAAAATAAAGCTGAAACAAAGGTGAATAGAGCAATTCATCACTACGTTCATACATCTTTGCTAGTAAAAGTTCAACCAACTGGTCATTAGTTTCCAATTCACAAACAACTTTTGCAGCTTGAATGTGGTTAGCTAATGTATTAGCAATGGTGGTTTTTCCTAAAGCAATCATTCCCCCGATCACAATACAATTAGCAATTTTATTTGGTTGAAAATGGGGCTTTTTTAGTTGCATATAAACACTTTTTAATATGTTGGGTTAAGGTTTATTTTTAATAAAAAACCATCACACTCACTGACAAGTCTTAATGCTGCTCCATTTCTGGCCTGACATGATTCGACTCTAATGATGTGATGGTTCTAAATTCTAACAATTATTTAAATCTTAATCAATTAATTTACTAACCCTGTTAATAGAATTGGATTTGGGTTGTGTAGTGCCTCTAGGCAATGAACTTGAATTTGATTTTTTAGATGGTTTGATCGGTTCATTCTCTTGATCTAATTGCTGTTGTGACGCTGGAGTTTCTTGTTGATAATTTGTTGGCGTTGAATGATCAAGTTTAGTAATTTCAGCTATCTTTTTTGTTAGTTTTGCTTTGGTTTTTTCAAACTGCTTAATGAAGTTAGTAAAGCCAGTCTTTGGATTCTTAGTTTCATCAAAAATGGTTGCAATAATCTCATCAAGCTTGGTGAAATCAATTTCTATTTCGGTGGTGAGCTTTTGGTGTTTTTGAAGCGAATTTACAACTAACTTATATGCTTTTTTTAATGCTTGCTTTTCTTTTTCAGCGGTTTTTAAAAGTGATTGGGTTTCACTAATTTCCCCATCTTTAAGTGCTAACTTGTCTTCAAATACGGTTTGGATGTGTTGATCACGTTTATAAATTGCTTCATTACACTGCACTTCATATTCCTTTAACAATTCCTCTACATTAATACTTTCATGTTCTTCAATTAGTTTCTTATTGGCTTCTTTTATTGCATGTAACTGTTTATTTAATGCTTCAATTTGGTGTTCTTTTGCTTCTACCAGCTGCTGCATTTGGATAATTTCACTTTCACTTTGTTTGAGTTGTGAGAAAAGTTCGCTGTTCTGTTGAACTTGACGGTTAATCTCTCCTTCCAAGCGTACCTTAAGTTGTTCTTGCAAACTTAAGATCCTTTTTAAACGTTCTTTTTCACTTCTCGCTTCAGGACCAAACTTTTTTAATAACAAAACAAGCATCTGGTTCCAATCAACATTTTTTGGGTTGCTTGTTCGTAGTTTGACATAAGCAGTTTTTGCTTGTTCTAAAAAAACATCAAGCTGGGCTTTGGAAGCAAAGGGAAACTTGGCTAAAAAGAAGTTAATAGTTTCTCTTCTTTGAAAGTCATCAAAGTCTCTATAGTAATCAAAATTATTCATGGAAAGCTAATTCTCTAAAGAAGCTAATGATAGCATCAGGATCATTAAAGTCTAAATCATAATGGTGTCTAACATAAACACTAACAAAACTAAAGATTGCTAAAACAAAACTAGTAAAGAGAAAAAACGGGAGTTTATTGCCATGGTTTTTAACAAACAAATCTAACTTTTCATTAGCAATTTTTTTAAAACTAACTACATCACTAAACTGGTTAGGATCTAGTAAATAGTTGTTAAGATCACTAACTTTACTTTCAACCCATTCCAAGTTAGCTTGGTTATATTGAAATTCACTTTTAATAGTTGCAAAATCAGTTTTTTCAACCTTGTTCAAAATTGATTTGAAGCCATTTGTGTATTTTTGAACAAAACTATCCATTTTACTTGTTATCAAATATTACTCCTAAGAGTTGCTCAGGTTTTAAACTGCCAAAGTAATCACTAAAATGATCAAGTTCATTGAAGAGTTTTTCAAAGGTTTTATAATCGTACTTCTGACCAATTAGTTTTTTTGTTACTGGGGTGATATCAACAACACTTAAAAAGTCCCCATAAAACTTAATATCAACAACTGTTCCTTTCTCTACTTGAACATTGCACTCAAATAAACCAGCATTATTAAAATAACGCTTGTTTTTAAAGTTGTATTCATAAGTTTTACCAAAGTTTCACTCCCATGATTGAAAGTGTTCTTTTGCTCTTTTTTCAACCTTTGCTAGTGCATCTTTAGTTAAAACAATTGTTTCTGCTTTTTCAGTAACAGTGAAAAAATTAATCATCTCTTCTAAAAATTTTGCTGTTGTTCAATTTGGTAAATACTCCTTTACATTAACAACGCGCTTAGCAACACTGTCAACACCCTTACTTGCTATCTTGGTCTTATCAACATTTAAATACTTTGCTAACTTAGAAAAGTCAGTGTCAAACAATAATGTTCCATGGACTAATAACCTGTCTTTAGCGATATATTCAGCTAACCCAGAAAACTTCTTGTTATTAATCTCAAGGTCATTACGACCATGAAATACAGCAGGTACATTTAAGCTATTTAAGAACTTCACCACATTTCTTGTAGTTTGTTCATAAGCATTTTCCATCACTTTACCTGTTCTTGGCAAAATAATAGAAAAACAGATGTTACCAAGGTCATGAAACACCGCTCCCCCGCCTGAAAAACGTCTAAACAAGTTAACCTTATCACTTTCCAACTCCTTTAAGTTAACCTCAGCATAAGTATTTTGGTTTCTTCCCACCACAATAGTGTTAGCGTTCTGCCAAAAGTAGATGACCTTAACTAACTCATTTTTTCTAAATTCAGTTAGCAATCACTCCTCTAAAGCTGCATTAAAATACGGATTGAAAACAGGGGAAGTAATAATGAAAGTTTGCATCAACTACCAATTAACTAAAGTAATCAAAGATCGCTTTTTTACAAACATCAGTTACCATTTCATTCATGGTAGGATGGGGTGAGATAGAATTGGCAATATCAAACACAGTGAGGTTGTTTTCCATCACCAAAGCAAGCTCAGCGATAATATCACTAGCAGTGCTAGCAATAATACATCCACCTAAGATAGCACCAGTTTTAGGATTAAACATCATCTTGACAAACCCATTGGTTTCATGATCTGCAATTGCTTTACCACTATAAATAAATGGCAAGGAAGATTTGACATAATCAATCTTTTCTTTTTGCAATTCCATCTCACTATAACCTACAAAAGCAACTTCAGGATTTGTGTAAATACAAGCAGGACACTTGTTTTTTTCAGCAGGCTTTACCTGGTTTTGGTTCAAAATTTGATCAACAGCATATCTGCCCTGTTGGTAAGCGTAGTGTGCCAACATCATTTGCGTGTTAACATCACCTATTAGATAGATGTTTGTAGTTGATGTTTGTAGTTTTTCATTTAAAACAATTTTGTTGTTATGGTCACGTTTTAAATCAAGTTGATCTAAACACTCTGTGTTAGCAATTCTTCCTATAGAAACTAAGATTTTATCACCAATTACAGACTGTTCAACTCCATTAACTGTGTAAAACAGTTGGTTGTTTTCAGCTCTAACAACATGAGCATTGGTAATAATCTGAACTCCTTTGTTTTTTAAGGTTTTACTTATCAGTTCAGAAACATCACTATCACAAACCTCCAAAATCCTATCAACACCTTGGATAATGGTCACTTCACTCCCTAATGAAGCAAATAAAAAAGCAAACTCAACCCCAATCACACCTCCCCCAACTACAACAAACTTCTTAGGTACTCCCTCTAAAGCCAAAGCTTGGGTTGAGTCAATGATAAACCCAGCTTGTTGTGCTTTTTCAAACCCTGGTAAAGTTAAGTATCTTGGTCTTGATCCGGTTGCAACAATAATGTTGTTAGTGGTGTAAGTTGTGTTGTTTACTTGCACCTTGTTTTTATCTATAACAGTAGCTTCACCTTCAATACTTTCTACCTTAGCACCCTTAATAATTGTTTTTACCCCTGCAACTAATTTATCAACTACTTCCTGTTTTTGTTTTAACAGTTGTTTTCAATCAAGTTTAGCTTGACCATTAATAGTGATACCATAATCTTTGGCATGAACTAAATAATCAATAATCTTTGCTCTTTTTAACAACGTTTTAGTTGGGATACACCCAACATTTAAACACACCCCACCAAAGTATTGCTTTTCAATCACTAGGGTTTTAAGTTTATGTTTGCCAGCATACTCCGCAGCAATATAACCAGCAGGGCCAGCACCCAAAATAATTAGATCATAATCCATAAATTAAGCTACTGTAAGATCAATTAATTCCTCAATTTGTTTTGCAATCTCCTTACCAAACCTACCAACATCCGCCCCATCAACCCAGCGGTGGTCTGCAGCTATTGTTAAAGGTAAGATGGTATGAACTGCAATTCCATTTTCCACTTTAACAATGCGTTCTTCTAAATTACCAGTAGCAACAATACACATCTCAGGGTACTTAATAATAGGTGTACCTACAGCTGCTCCTAATGAACCGAAGTTAGTAACTGAAATAGTACCTTTATTCAAATCAGTCAACTTAATCTTTTTTGTTCTAGCTTTGTTAGCTAAATCAACAATTGCTTGGGCAATTTCAACCACAGATTTGGTTTGGGCTTGCTTAATGTTAGGGACAATTAAACCTTCTTCAGTATCAACAGCAATTCCTACATTAATGTCATCATTTAAAACAATTTCGTTTTGATCAGGATCATAACTAGCGTTAAAAACAGGGAACTTCTTAAGCGCATTAACAATTGCTTTAACAAAGAAAGCAAAGTAAGAAATTTTCATGGAATACTTACTTAAAGCATAACCATTAACACTTTCACGATATTGTTTTAACTTGGTTGCATTAACATAAAAAGTTAATACAGTGGTTGGGATAATTGCGTGCGACTTTGTCATTGCTTCTGCAATCGCTTTACGCATTGTTGAGATAGCAATGGTTTTCTGTTCAGTTTTACTTTCTGGTTTTTGGGTTGTTTCTACAGTAATGTTAGTACTACTTGCAACTTTAGTGTCTTTAACAGCTGGAGTTGCATGAGGTTTTACTCCAAAGATAGGAAAGAGGTTATCAGAAACCTTAATTTCCCCTACTACTGAAGCCCCCGCTTCTTTTACCTTAGCTACTTCTTCAGTTGGTTGAGGTTTTGGTTCAACAAGTGGTGTACTAGTCTTTTCACCAATAACTGCCATCACCTGACCAATGCTAACAACATCACCAACTTTAACATTAATAGCACTAATTGTACCTGCAAAAGGAGAAGGTAGTTCAGTTGTAACTTTATCAGTTTCAACAACAAATAAAGCTTCATCTATCTTGATCTGATCACCAACTTGTTTTAAGATTTCAGTTACTTTTCCTTCATGTAAACCCTCACCAACATCAGTGAATTTAAACTCATTTGCCATATCGTATTGATAGTACTTAAAGTTTAAATTTAATAGTAGTGATCTATAACAAATAAAAAAAGCCAGTTTAGTTACTGGCTTTTTAGTTGTTAGACAAAAATTTTGTCTTACTTTTTACAGCAGCTGTTGCACTTAGCGCATTCGCACTTGCATTGGCAGCATTTCTTGTTGTCTTCCATCACTTTAATTTATGAAATGAGGTACCTAGCTCTAAAAAATAAAGCTAGGTAACTATATTTTAAAAAAGTGTTATTTTAAAAGTTGATTAACTGCATCAATAACCCGTGCATTAATTTCAAACTGGTATTTTTCACCTCTAGCTAAAGGCACAACAATATCAAACCCAGTTACCCGTTGTGGGGCTTTTTTGAGATAAGTGAATAGTTCTTCAGTTACTGAAGTGATAATCTCTGCACTTGTAGTGAAACTTTTCACCGCTTCAGTCACTACAAGTAGTCTTCCTGTTTTCTTCACTGAGTTAAATACTGTTTGTTTATCTCAAGGGGAGATAGTACGCAAGTCAATTAACTCAATTCCCTTATCTTTCAATTCCCCGCTGTAAACTAAGTTAATTAAATCAAACATTGTAGGACCATAGCTAACTATTGTAAGTTCACTACCTTCACTAATCAAGTTGGCTTCACCAATAGGGACAGTGTAATAATCACTAGGAATCTCCTGACGAAAAGCACGATAAAGCTTCTTTGGTTCAAAAAAGATAACAGGATCAGGTGATTCAATAGCAGCTAGAAAAAGTCCTTTGGTATCATAAGGATTTGATGGCATCACTGTTTTAAGCCCAGCAATCTGTGCATAAATTGCTTCCAATGTTTCACTGTGGTGTTCCAATGCTTTAATCCCCCCACCCATTGGCATCCTCACTACTAGTGGAGCGGTATATACACCACGAGAACGGTTTCTAATCCTAGCAGCATGGACAAAGATTTGAAACATAGCTGGGAATGAAAAGCCTGAAAACTGGATCTCTACAATAGGTTTAAGACCACCTATAGCAGCCCCAACCCCAATACCAGCCATAGAGTTTTCTGCTATAGGACAATCCCATACCCTTTCACTCCCATACTTTTGTTGTAAGCCTTTAGTTGCACGGAACACACCCCCTTCAAAACCAGCGTCCTGGCCATAGAGTACAACGTTTTGATCTCTTTCCAGTGCAAGATCCATTGCGTTGTTTAACGCTTCAATGTTATTTACTTGGATTTTTGACATAGTTATCTTTTTTTAAATTAGTCTTTAAAGTATTTTTTTGCAATCTGTTTCTGTTCAACTAGTTCAGGGGTTAATTCTTGATAGTTGTAATCAAACACCTCATCTACTGATACAGGAGTATCTAGTACCATCTTTTCATAAGCAGCTTGGATTTCCTGTTCAATTTTGCTGAACATCTCTTCTTCTTGAGCTTGGTTAAGAATTGATCTATCAAACAAGAAGTTTCGCAACCGCTTCACTGGATCACTCTTCATTCCCTCCTCCTCTTCTTGTTTGGTTCTGTAGATAGAAGGGTCATCTGAAGTGGTGTGAGGACCTTGCCGGTAGCTGAAGAACTCAATTAAGACTGGTCCATTACCACCTCTAGCGTAATTAGCAGCATCTTGCATCGCTTCATAACTAGCAATTAGATCATTACCATCAACCCTTACCCTTGGGATCCCACATGCTATTGCTTTAACGCTTAGATCACTAACAGCAGATTCAAGTTTAGTTCTTGTTGAGATAGCAAACTGATTGTTGTTAATACAAAAAACAGTGTTTCACTTGTGGATGCTTGCAATGTTCATCGCTTCATAAAATTCCCCTTCAGCTGTACCTCCATCACCAATCATAGTAACAGCAACATTAGGTTGCTTTTTATAGTGCAACATGTAACCTAATCCAGCAGCATGGGAATACTGAGCACCAATGGTGATGTTAATAGGTAAAGTTTTGTATTTAGCATCTATCTGACTACCTTTTTCATTACCATTTCAGTAGAGTAAAAGTTGTTCTGGTTTTACCCCACGATACAACATTAAAGCCCCACTACGAAACGTAGGACAAACCCAATCATTTTCATTTAAACCTAATCCCATTCCAACCTGTAAAGCTTCCTCTCCCAAATTAGGAGCGAAGTTTAACATCTTACCAGCACGCTGTCAAACTAACATCTTTTTGTCCATCATTCTACTCAAGTTCATTAAGTAATAAGCGTGTTTTAACTGTTCATCAGTTAGGGTAATTTTGTGGTTAGGATCAATTAATTTACCCTCATTATCATAAACCTGATAAAGGGTAGTTGGAACTTTATTTTTAATCAAGATTGCCATATATTTCTATGCTAATTTAAAACTAAGCTATTTTTTAGGAATGTAATAGCTAAAACCAAGTGCTTGTAAAACAGTTGCTAACACAAAGTTAAACGGTTTGTTGTAATGTGGAAGAAAATAAACATCCACTAAACCCAGTTCAGTTAGTAACATCTGCTTTTGGATTGCAAGTGCAATATAGAAAATAATCTCACTGTGATTGGTATTCCAAGAAAGGAGTTGTGCTCCTAAAATTCTTAGGGTTTTCTTGTCATATACAAGTTTAAAACGCACCTTGTCATAACTGCCCATAAACTCAGGACGATCATTGTCATCAACAACTGATATGCCAACATCAAAACCTAACTTCTTAGCACGCTGTTCAGTTAATCCACATGCTGCTAAATTTAAACCAAAGATATGGAGTGCATTGGTGCCAACGATAGATTGGAGTTTAACTTGGTTACTACCAATGATATGCATCGCAGCTACTAATCCACTCTTTACTGCATTGGTAGCAAGATCGATGTTTTCATACTGTTCACTAGCAGCATTGTAAATAGCAGCACAACCCCCAATGACATAAACATCCTTATGATTTAGTGCTTGGAGAAATTCATTAACTTTAATTGAACCGTTGTGAATAAACTCAAAGTTTTGATCTTTAGGAACAAACTTAGTGCTAGGTCTAAACCCAATTGATTGGATCACAAGGTCTGCATTTACTATTCCCTTATCAGTTTCAACTCCTTTGACAACGTTGTTTGTACTATCAACAACAAAGCCCTTAACACTGCAACCCATCATTAGTTTTAACCCATCTTTTTGCATCACTTTTTCAAGTTCATCAGTAAACTCATGATCAAAGTTATTACCAGCAGGCTTATCAAGTAAGTCAATTACTGTTACTTGCTTTTTGCATAACCAAGCTGCTTCAGCAAGTTCCAAGCCAATGTAACCAGAACCAACAATAGCAACTGATTTAATGGTTTTATCTTTACGAAAACTATCGATTAAGGTAAGTGCATGTTGGTATAACTTACAGCTAATTAAGTTCTTAACATTACCACAATATTTGTCAGTGTAGTTAAACTCCAAAGGCTTGTGTGTCACCTTGTTTTCAACATTCATACATATAGGTCATGCTCCTGAAGCGATTACTAGTTGATCAAACTGATCAGTGAACTCTTTATTTGATGTTAAATCTCTAACTGTTACCTGTTTTTTGATTAGATCAATGTTAGTAACATCATGACTCATAAAGATGTTAGCGCCCATCTGTTTCAACTCCTCAGGGTTGGAATAGAAAAGATCATCAGTGTTTTTAACAACACCACTAACAGCAAGTGCAATTCCACACCCCAGAAACGAGATGTTTGTGTTTCTATCATAAGCGTTAACCTTAAAGTCCTTACTTTTTGAAAGTAAAGTTCTAATAAAACTAGTACCAGCGTGATTTATTCCAATCACAATCACTTTTTTCATATATCTTAGCTGCAGTAATTTTTTAAGCTATTTAAGGATAATTTGTGCAGTAGTGATTCGATCTTTTAAAATTAAGCTTTTGCTAGTAATGTAATAAAATCTTGCTAACCACATTTGGTTGTAACTTAGCTTTACCATTGAGTTTTTTCAGCTCAATTAAAAAGCAATATCCCACAGTTTCACCATTTAACTGTTTAAGTAATTGGTCAATAGCAGCAACTGTTCCAGCAGTGGCAAGTACATCATCAACAATAACACACCTTTTAGCATTATTAGCTTGGATTAATGAAGTGGTTGACATCTCCAATACAGCATGTTTTCTGTACTCCAAATCATAGCTAGCACTAATTAATTGCCCTGGGAGTTTATTGGCTTTTCTAACCAATACTAACGGGAGTTGGGTTTTAGAAGCTAATGCTCCCCCAAAGATAAAACCCCTCGCTTCAGGACATACTATCGCTTCTGCATTAATAGCTTTAATAAACTGTGCCATTTGGGTTAGCACAAAATTAAATAGTTGGGGATTGGAAAATACTGGGGTAATGTCATAAAACAATGTACCTTGGTTGGGAAAATTTTCAAAGCGCTTGATTGCTTGATCAAGCAACTTAAAGTTTTGATCCATAAATATCTTTTTTTTAAAAACTGTTAATTCCTGCAATTAACTGCTCTTCAATCTGATCAATCTCTTTAAAAGGATGTTTTTTGTGTTTTAAGCTCAAACGGGAATGAACATAGTTGAAAGGACTGAGTCAACCATTGATTAAACCAAAGCAAAACATTGGTAAAAAAGCAGTTGCAACATCAAATAAACCAACAATAGCAATTAGATAAAAGATCACAAGGTTAAAGTTGCCATCAATCCCACCAAAGTTAAAACCACTGTAGATAATAAAAATGAAAACTAAGCTAGAACTTTCAATCAGATCTATCTTCCACTGCGAGTTGAAGTTATCGTTAAATAAGTTTTTGATAAATACCTTGTCCTTTAAGCTCCTTTTAAACCAGTAAAAGTAAGTCCAAGTTGTTGTTTGTGATACCTTAGCTGCAAAAAAGAGCCAAAACAGATAAACAAAGGTAATTAACTGGATTGTTGATTGGTCTATACTAAACAGATAGTTAGTTAGATACATCACTGCAAACAAACCACTACATAACAAGATAAAGCTAACCAAATAACTTAAACAATAAGCAACACCATATCTAATCAAACAATAAGCTAAGTACAGTAAACTAACTATTCCTATCCCAGCAATAATCCCGTTACTTGATTCAACATCTGCTGCTAAAAATTCTCCTAACAGGTTAGGTTGAACAAGATATAAAACTAGCATTACAACTCCTATAGCTAACAAGATTAGCCATACAAACAAAAACTTCCAGTTGTTTTTCAAAAAGAAATCATGTTGGTTTTTGGTTATAAAAAAGTCTGTGTTCAAGTCACTAGTTGAACTTAAAAGTGAATAGTTACTAGAAGTTTTAGTTAGTGCTTTTGCATCCTTCTGATAGAGAAAAAGCTTTCAGTTTGCACCTGATTCTGAACTGACAAACAGTGAGATTAAAACGATGCTGATTCCATAACTAAAAAAGTAAGAAGTAATGGCTGATATTGCCATCAAATTAGCTAGTTGTTGGACCTGATAATTAGAAAGATAGATAACAACTAAAGCACTAATTAACCAGGTAATGTGAAACTCAAGATTAGCAAAAAAGCTTCTTTTTAAACACAGTTTTCACGATTCGATAATAGAAGCATTATTTCTAATATTTCTTAAGAATAACTCTCCTAAATTCAAGAGATTAATTAGGTTGATGGCAATAACAAAGAAGATGCCCACAAAAGAAAAGACATCAACAACTCCACCAACTGCACTAAAGATAACCAGTGATGACACCACACTTAACCCTAAAGCTAATGCTTTGTATAACCCTAAGAGCTTGTATCGTAGTGTTAATAGAACAGCAGCAATAAGAACGATGATACCAAACGCAATAAAACTAGAAGCAAAATTAGAAACATCTGTGATGGTATTTGCAAGTGGAGCGTTAACCAGACTTGTTTTTGCAGTTGCAATGGTAGCTGCTGTGGGAAATGGATTTGTTAAAAGCTCTTTAACAGTAGCTGCTGTTACAAAGTTTGGTGGGGTGTATGTACCATCACTACTCAAGAAATTACCAACTTGAAACTTAATATTACCACCATATTCTATCCGTTGGTTGTTAGTGTCTAAAAAGCTTTGTAGAGTAGGATTAATCTTAACAGGATCACCGGTTTTGCTAACCGGATCTTCAGTTCTAATTTCACTGATAATATAGTTATGGAATAACCCTGAAAAACCACTGCTATCTGCACTCCTTAACTTTCTTGTATCAGCACTATTTGAAACTGTAATTACATCTGAACTATTGTTGTTATTGTTATTATTCTGGTTATTATTGTCATTACTGTTGCTAGCAACCGCTGAAAAGTGGTTAGGAGCAGCGTAGATATAATAAAGGTTTTTGAGAGTAATGTCACTTGCTTTTTTAATAGGAGATGCCCCATTAGCATCAGTGTTTAAAAACAGATTATCACCACTAGCAAAGCTACCCTTTGCATGTAAAAATTCCCAGAGACTCTTCTCTCTTTCATTAAAGGTGAGATAATCACTACTACCCTCAACTACATTAAAGATATTACGGACATAGTTTAATGCGCCTTGTTTATCTTTTCACAATACTAAAGTTTTATTACCACTACTTGAAGTTCCATTCGCATTTTGCTTAAAAAATTTACCATTGCCCTCAACACTAGCTTGCCCATTACCAGTATTATAATCAGAACTTGATAAGTTTTTAGAGTTGATAAAATCAAGAAAATCGGTGTGTAAACTTTCTTTGGTAACTACATTTTTATTTAACTTGTATTCAAAGGTTAAAGCACTGTTTACAGAAGTGTTTTGGGGAACAACTGCCCTAATGGAAGCATTAATAAAATTATTATCCAGTGAATCAAATAAAACCACTCCATCAGTTGTTTCAAGTGAAAGTTGGTAGTTGTGTTCAACTGAACTAATTGCATTTCTTCTAACCTGAGCATCAACTTGTCTTTTTCTTTGGCGGGTTTTGGCTTGCTCACGGTTTTGTTCAAAGGTGAGAGTAACAGAAGGTAATCCCCCATTAACCAATCAGGACTTGTCAATGTTGTCAAGTTCACTAGCAGTTTTATCATTAGTGTTTTTAGTAATGTTTACAGAAGAAAAGCCCTGAATGAATAAACTGTTAGCATAACTTTTTTCAACACCATCTAAAAAGCTATCAATGTTAGTGATGTTTTCAATCCCGTTGGTTGGTTTTGTTTGCTGAGGATCTAATGAGGTATTGTTAGTTGACTTATGGTTTAAAAAGTAAACAGTTGTAGTGGTAGAACCGTTGAAAACAGCACCTAACCTGCTGTCATTTAACAGTTTGTAACTCCCAAAAATAACACCAAACAAACCCAGACAAACAAGTCCAAGAATAGTTCCTATTTTTAGGATTCAGTCCAAAGAAAAGCGCTTTTTGAACCTCACTAACTATTTTTAATAACTTAAATTTTATATAAATATTAAGTAATGGCAACCATTCAGGAAATCGAGTGTGATTTTTTAGCTAAAATAGCACAAAAATTTACTAATGCAGAGATTGAATTAATTAACAAAGCATTCTATCACGCTAAAACTTGGCATGAAAACCAGAAACGGCTTAGCGGTGAACCTTTTTTTATCCATCCTTTAAGAACGGCATTATCACTAGTTGAATGGAACATGGATCCTATCACTATTTGTGCTGGTTTGTTACATGACATCATTGAAGATACAGACCAAACCGAAGCTAATATAGCAATGATTTTTAGCAAAGAAATTGCTGAGCTTGTCACTAAGGTTACAAAGATTACCAATGAATCTAAAAAGCAACGTCATCTCAAAAATAAAAAGGAGAATCTTAACTTAAAAAGCTTTGTTAACATTGCAATCAATTCTCAACAAGAGATAAATGTAATGGTACTAAAACTAGCAGATCGACTTGATAACATCGCTTCCATTGAGTTTCTCCCCATTGAAAAGCAAAAGGTAATTGCAAAAGAAACTTTAGAACTTTATGCAAAGATTGCTGGGAGGATTGGGATGTATCCTGTTAAAACAAAATTAGCAGATCTTTCATTTAAGGTGTTGGATTTAAAAAACTATGATAACACCCTGTCAAAGATTAACAAGCAAAAGGTCTTTTATGACAATGAGTGGGATAACTTCAAACAACAATTAAAAAAAATCTTAGCGCAAAATCAGATAGAATACCAACTTGAAAGTCGGATTAAAGGCATTTACTCTACATATAAAAAACTAACTGTTCATGAACAGAACATCAGTAAGATCCATGATCTTTTTGCTATCCGCTTAATTACTAAATCAGAACTTGATTGTTATCACATCCTTGGTTTAATTCACCTTAATTTTTTAATTGACAGTAAATACTTCAAAGACTATATTGCCTCACCTAAACAAAACCTTTACCAATCAATTCATACCACTGTTCGTTTAAAAGGGTTAAATGTTGAGATCCAAATTAGAACCCAACAGATGGACAATGTTAGTAAGTTTGGCTTAGCTAGTCACTGGATCTACAAAGAACAGAAAGAGGGATTGTTAGCACCTGCTTTGCAACTTAATTACCTAGTGACAAAACAAAAACACTCACATGATTTTCTAAAAAGGATTTTTGGGACTGATATTATCAAGATTAATGTTAGTGCTAGTCATGAACCTAATGTAATTAAGCAAATTAATGTTGATAGCAACAATAAACTCCTTGATATTGCTTTTGAAAACTATCCCAAGCAATTTGCTAAATTAACCAAAATTGAAATTGATGGGGTTGAGATCAATTCTTTTGATACTAGTGTTGAAAATGAGATGCTGATTGAATTTTACTTTGGCAAGAATAACAATTTGAAATCAAAGTGAATTAGGTATATGAATAACCCTATATACCGTGAAAAGGTAAAAAAGAGCTTGGCTAAACTAGCTAAATCTGGTAGATACAGTGAGTTAGCTTTTTATGAAAAAGAACTGGGTGAAAAACAGTTAAAACTTGCTAGTGAAACTGAAATCCAAAAACGCTTAAACACCCTAAGAATTAAAAAAATGAGTGATTACTTAGCGTTAATTGAGTGTACTAACTTTACTAATGATGAACATTTGTTGTTTCTAGCTAAAAACAACGACAAGTGAAATAAACTAACAAAACCACTTAAGTTTGCTTTTTCAAAAGTAGTTTTTCACAACTCTTACTTTGAACAAATTGAAGGTATTTTTATCACCAAAATAGTGATTGAACCATGTTGTAGTAAGATCCCTGATATGCCTGAACAAGTAACTGGTATCTTAACTAAAAACATTTTAAGTGTTCACCGTTATGGTTGTAAGAATTTACAAAATAAAAAGCAGTTAAAAATTATCCCGTTATATTGAAATATCCAGCAGTTAAAACTAAAACCACGTAAGTTTCGCAGTTACATTAACATTAACGGAGTGTGGAGTGAAAAAACCATTAATAAAATCTGTCAAACAATTATTAATGGTGATGGTTATATTGAAAAAATAATTCCCAAGATCAACAAACAAAAAGATGAATTTGATTTAAACATCACCCTTTTTGTTAATAACTACCAACAACTTCTCACCTTAATGGACCAAATTACCACTAAGAATATCAGCTTTAGTTGAAAATACCTTTAGTTACCAAACACCGCATCATACACTTCTTCATCTCTTAAAAGTAAAACTAAGTGCTTTTTTAGTAGCCGTTGTGGATTGAAACGTGATACTCTCACTCCATAAACTTTCTTTGTAAATAAAATTAATAAAAAACCAGAAACAAAAAATGCCCCAATAATTGAGATTCCTGTATAGAAAAGGATTTGGGAAACTTCAGTTAAATACCGCTTAAAATCATCATTTTGGTTTGGATTTGGGAACTTTTCTAGCATTTGATCAACAAAGCTAGTTATGTTATTTAATGTGTGTTTAATTTGGGTAAAAGCAGCATTACTATCAAAGTTATTAATTTTTTTCAATGAACCTTCAAGTGCATTTAAAGAACTATCTAATCCTGATGCAAATGAATTGTTTTGCGTTAATTTTCGCCCTTCGTTTAAAGATTTTTCAACTTCTGAAAGTGTTTTAACTAGGTTATTTTGGAAGATATTTTGGGAGATCTTTTTAATTTCAGTGTTATATTCACTAATCTTGTTATATCCCTCTTTATAGTAGTTGTTAATAGGTTCACGGTAAGTAAAAGCAAAATAACCAGCAGTAGCAATTCCACCTAACAATAAAATCCCTACCAAAATAATAAGAAAAACTGCAAGTTTTTTTAATAGTCTAATCATGGTCTAATGCTGTTATCACTTCCTCTTCCAAATCAGGATAACGATCTAAGATATCATTAACATGATCAGCTAACTGTTCAGTACTAATAAATCTTGAAAACCTGATCAATCCTCTTTTTTTAGAAGTTAAAAAGGAAAAGACAATGGTTGTAATCAAAATTGCTAACATCCCACCTGATACAGCAACAATGGTTATCATTGTGACATCATAATAATTATTGAACTGTTCAGAAGAAGGGATGTTGTTTTTGATCTTTTCATAGCTACTAACAATGCTTTGTGCTTGTGTAGAAAACTCACTAACTGCACTAATTACACCATCAGCTGTTGCTTGTTTTTTTACAGATGGAGATGAACTAGCAGTACCATTTTTATCAAGATTATTTTTAAAGTTGTCTAATTGTGTTTTACTGTTTTCCAAGTCTTTTTTGGTTGCATCAAGTTCCCTAATCTTTTCTTGACTAATATTACCTAGATTGTTCTGACTCTCTAAGTTCTTTTTGTATTCATCCACTTTTTGTAAAGATTGCTCTAAGTCTTTTTTAACTTGATCTAAATTGTTAGTAATGGAACTTTTGCTAAATTGGTTAGCACTATTTGCTAAAGCATTAGCAAAGGCTTTAGCAGAATTGAAAGAACTATCAATGGAATTACGAGCTTGAACATAAAGGTTAGCTGCTGTACTACGAAAATACAAAGCAGTAATACCAAACCCTAACAGAAATACAAAAACCAACATTCCAAAGAAAAGCAAGATGGTTTTCGAAAACCGATTAATAAAAATAACCATTTAAATACTTGTACTTTTAACCAAACTAATGGTTATAAAAGTGTTTTGAAAACAAAACTACTAGAGTTTAAGTTTAACACCCAATGCGTTGGTTTTAAGTTCTTTTGCTAGTGATTTAACTTCATTTGTATCACTGGAAACACCAGTAATATCAACTGTTTCAAACTTTTCTTCACCAATTACAGTAACCAACCCTTCAACTGCTTTAGTCATTCTTTCAAGACTACTATCTGTATCATTTTCTAGTTTTTGGAACTGTACACTGTATTTACCACTAGCAGTAATTCTTTTGATGTTTTCATCAAATGTTACTCCGTTTTGTTCAGCACGTTTTAAGATTTCATTTTGAAACTCGCTGTCTCTGCTTGCGCGTTCATTTTCATAGATGCTAGCACCAGGGTAATAGTTTTCATTAACCCTGTAATAGGTGTTATCTCCTTCTCTGTAAGCTTCTTCTAAGTGGAGGTTAAGTTCTTTTAAAGAACGGTACACCAAATCATCATCACTATCCTTGTTGGTATTAACATTCTTAACTAAGTACTTGTCAATGTATCCCCCAGCAAAATAACCTTGGAACTGTCTTTCAAACCTTCTGTAGTTGTAAATATCACCAACTGCTTGTTTGAGCTTATTAGCATCAATGGCTGAATTATCACTGTTTTGGAGTGTAACTTGGGTTAAATCAATGTGGGTAAATGGTTTTGAGGCAAACAGATCATAGATAACATTAGTTTTACTTCCCAATACTAATGGGTTAAAACCAAAACTATTTGCTTTACTGGTGGAATAAAATTCCAACTCAACTACGTTTGCAAAGATTTGTTTAGCTACATTAACACCAGTGTAATCACCATAAAGTGAAACTTTTTTAACTGATTCTGGTAATTGCAATTCATTAAGTTGTGAACCATTACCACTTAAACCTCTAATAATTAGGTAAGTAGGTTTTTCTGAATCTATTGTTGTTTTTAAAGCACTGATATCTTTAGCATCAATTGCGAGTGTTTTAACATCTTCAAAGAAGTGGTTTGGGTATGAGTCATAAAGTGGTGAACCTTTAGCAGCAAAATAAGTTCACTTATCATAAGTAAGGTTATTACTAGTTGAAACAGTTGTTGCTTTTGTGTTACTCCATCCGCTGAAACTAGGAACTTGTCTAAACACATCATCATAACCACGGTTGTTTGCCATGAGAGTACCGTTAGTTGTTTGCAAGATTGGCATCAACACGCGGTTATTGAAATATTCAGTTGATTGGTAATAGCTAAGCAGTGAACCATCACTAGCAACATGATCATAATAACCAGCTTTAAAACTAGGAGTAATAAAGTTATCACCTGTTTTAATTAAGCCACTAATTTCAGTTTTAGGAACAGGACGGTCTAAACCCTTTGGACTGTTAGTGATCGCTTCTGATAACTCATTAGCAAAGTTTCTAAATTTCTCACGGAAGTTAGCACCACCAGATAAATCAATCTCACTCTGATAGCTACCATCGTTTAAAGAAAGTGATTTCAACTTTACTTCTGCACTAGGAGATTGTGTTGAGAACAACTTAAAACCGAGGTTACCACTCCTACCTGATGCAACCAAAGCATAACCACTTTGATTAACTAGGTTAGTGATCATAACTGCTGAGAATGTGGTTAACGCTCCAGCACCTAAAACACCAATAGTCCAAAATAATTTACGCTTAAATTTCACACTATTTTTATGTTTTTTAAATTGCATTATTTTTTGTATTATTAGAAACTTTTAATTTGTAAACAGTAGTCTACTTATCAATTTTAAATTAAATCCACTAGTAAACTTCAATAACCCTTTCTCTGAAAGAGCTTGGTAATTTATTAAACAAATAAAAAACACCCTTAAATTGCTTTAAGGATGTAATGAGTTTAAATCTTTAATTTTTGGAGCGAGTAATCGGAATCGAACCGACACGATCAGCTTGGAAGGCTGATGTTCTACCATTAAACTATACTCGCACAAATTTGGTGCCCAAAGCCGGACTTGAACCGGCACACCATTACTGGCGTGGGATCTTAAATCCCATGCGTCTACCATTCCGCCACTTGGGCTGGTGACCCACCCGAGGATCGAACTCGGGACCCTTTGATTAAAAGTCAAATGCTCTACCGCTGAGCTAGTGAGTCTTTTTGATAGGGAGTTAACCCTATCTTTAGAAATTTAAGCTTTAACTGGCTGGGATGGATGGGATCGAACCATCGCATGATAGAGTCAAAGTCTATTGCCTTACCGCTTGGCTACATCCCAAAAATGGTGGACAGGGAGAGATTCGAACTCCCGAAACCATAAAGGTGTCTGATCTACAGTCAGAAGCGTTTAGCCACTTCGCTACCTGTCCATTACTACCAGCAAGATATTTTACAAATTATCTGTTGCTGATCTTCAGGATTTTAACACGATAGGGAACTTCAATACCAACAACATCACATTCATCACCAATTAAACGCCCATAGATTGCAAGGGCAAGGGGTGATTCATTGGAAATTTTGTGTTCTTCAGGATTTGCTTCAAGTGTACCTACAATTGTGTATTTTTCGTTCGATTTAGAACTGTAATCATAGATCTCAACAGTGCTCCCTAAGCTAACTTTTGTTACTTTGGTTTTTGCTTGGTGGTCACTAATTAACTTAGCGTTGGCTAATATATCTTGAATTTCAGCAATCCTAGTTTCAATCTCACCTTGCTGTGCTTTAGCTGCATCATAATCAGCATTTTCACTTAAATCACCCTGATCACGTGCTTCTTGTAAGAGTCTGATAATCTCAGGACGTTTAACTTGGATTAGGTTTTCAAGTTCTTTTTCCAGTTGCTTAAATCCTTCTTGAGTTAGGTAATTTTTATTTAGTTCCATGACAAAATTAAAAGCAGCAAAAACACTTTTATTATTTAATTATTTCCCCTTTTTTTGCCAATTTTTGCTAAAAAAATAGAAAATCAGTATTGCAGTAATATAGATATTAAATAGTAAATTTCTATTTAACTTCGAAATAATAAACTCAAGATCAACAGAAGATTGTGAGTTTTAATACAGATGAGAGAAAACAATAGTAATGCTCAAAACAAAAAGAAGATCCGCTTTTTAGCTTTGGGCATCATTATTCTAATCGTACTAATTGCTTTAATTACTATGCTTTTTATCACTGGAGTAATTAGTCGCAACCGTTAAGATTACTTTTTGCTAGATGACAAAAAAAACACAAGATCTCACTAGTTGGTATGACCAACTGCTAGTTAAAGCAAAGTTAATTTGTCATGGTGAAGTTAAAGGTACAGTTTGTTTTTTAAATAACAGTTGAGGCTTATGGATGGAAATCCAACAGCTTTACAATGATGCAATTGCAAATAAAAATCAATTGTCTGCAATTGCTCTAACTAAATTCCAACCAACTACTAGTTTTTGTTATCAAGTATTCCAAGTACAACTCCCTACCCTTTCTTTTTACAGTGAATATCAAAAGGAAAAAACCCATATCAAAGGTTTTAATCCTGAGCTTTTTTTAATTAATCAAGTTGGTCAAAAACAACTCAATGATCCTTTGGTTTTACGACCTACTAGTGAGATTGCTTTTTGCAACTTATGGAAAAAACAAGAGTTATCTTACCATGATCTACCTTTAATTTATAACCAGTGAACTCAGGTTTTTCGTGCAGAAAAAAACACCAGACCTTTTTTGAGAAACAGTGAGTTTTACTGACAAGAAACTCATGGGCTTTTTGTGGATCAGAGCCAATCTGAACAAGCTGCTATTAGCTTTTGAAATTTATATCAGGATTTAATTATTAACAAACTTTGTATCCCTGCTTTTGTTGGTTTGAAAAGTGAAAGTGAAAAATTTGCAGGTGCTAAAAACACATGGACAATAGAAGCAATTATGCCTGATGGACAAAGTTTACAATGTGCCACTAGCCATGATTTAGGTGACACTTTTACAAAGAGTTTTACTATCAGCTATCAGAGTAAAACTAACCAAAAAATGACTCCAAGTAGTTTTAGTTGTGGGATGTCAACTAGGATCTTAGGAGCAATTTTTTTAACCCACAGCGATGATTATGGTTTGGTTTTACCTTGGTATCTAGCAAGTAAACAAGTCAAGTTATACCTGTTTGATAAAAACAATAACCCTAAAACAAGAGCTTTAGCTTTTTTAGTGAAGGATTTTTTAGAAAAACTCAAAATTCGCTTTAGTTTTATAGAAATTAACAATCAACTAGGTAAACAACTTTTAAAAGGAGAAATAGAAGGTATTCCATTACAGATGATTGTTGATAATGAAAAAACTATTAACATCTTCAACCGCTTAACACGTTTAAAAACCAGCTTAACATTTGCAAATCTCCAAACTGAATTTGTTAATTTAGTTAACAACTACCATACAGAGATGTATAGAAAAGCAAATGATTTAGTTGAACAAAAACTAGCAAGAGTACAAACTTTAAAGGAAATTGAACAAGCATTCAAAAATAAAAAGGCTGTTTTATGTACCGTGAAGTTAACTGGTGAACTTGAACAACACTTAAAGACAAAATACCAAGTTAGTGTTAGGTGTGTTTTTAAAAAGTCAGATGTAACACAAAACTGTCCTTTTACAAATCAACCTTGTTTTGATTCAGTTTTAATTGCACGTGCTTACTAACAAAGTACTGTCAATCACTACCCAATAAAACCCTTGATTATTTGGTTTTATTTAATTTATTTCTAAATATTTTTTACCAGTAATTTTTGTTTAAAAACAAACTCGTTTTGTAAAAGAAAAAAGTTAGAGTTGTTGTATTTCAATAACGATTATTTAGCCTCTAAAATCGATTAGACGATCTTCTGATCGTTTAAGATTTATTCAAATTCAACATGACTTTATGACTTTTACAAACAAAGAAAAAAATTGCAAACAAAGCAAATCATTAGCAGCGTTGATGACAAAATTTAAGCGCTCGCAATTAATCTTGAAACACCAAGCTAATAACATAGCACTGGAGTTGTGAAATGAAAATGATATTAACCTATCAAAACAGCTTATTGAGTTAATAGAAGATACTTTTTCAATGCTTAAAAAAGAAACTGTTGATTTTATCTATGACATCTATATTTATGGCAAAAAGCCGTGTGATATTGGTTATTCTAATTCCACCTATTACAAAAAACTAAACAAAGCAGCTAATAGTTTTTTTGACCATTTTGTTTGAGATTCGTCAATATTAGACAAAAGGATCATAACTAATGGCAGCAATTCTCAACGTACAACGAATTCAAAATAACCAAGTCACTGAGTACACAATGAGTCCAGTGCGTAATTTTGCAAACACAAAAGATGTATATTTTGATGCGCAATTAACAAATATAGAAAGCAAAATTGATAGTAGTAGGGCTCAAATTCACCTTACTATTGCACTAAAATACAACACTAATCTCCCTGATAATATCTTTCAAGCCCACTTCAGTTTAGGCAATTGACAAAGTGATAAGATTCAACTTCAAAAAGCTCCTGATAAAAAACACGATAGTTTAAACAGCATTAAATATTTTTATGCATTTCTAGATGTACCCCGTTCAGCACTAGCAAAAAAAGAAATTAATAGGTTTAGTAATGTGGTTGCTAGAGTATTGAGAATAAGTTTTCGCTTGCAAGATCAATCTGAAAAAGGGAATTGAAGTGACTATCATTTGTTTGATACTGTAGCTAGTGAATTGTATGCAACAGTAATTAAAGAAACAATCAACTTTGGCAACATGATTAAGATTAATGCACTTGATGGTAGTAAACAGCTAACTAGTAGCCAAGGAAGTTTTAAATACAGTTGAACAATGTATGACTATAGGAATTTAGAACAACTTGATGAGGTGAGAAACTTGATCAATATCAGTTTTGACAAACCAGTTCAAATAGTAAATGTTGATGTCAAAATTCACTATGTTCCAACTAAAGGTAGGTTACAAGAGATAAAACAACAAGGTGAGTTTGAAAATAACCTTGATGTTAATGAAAAGCTTAAACTCAATTTAATAGGCAATTGAAATTTTGACAAACATAACAAGAAGCTAATCAGTGATATTTCAGGTACTGGAATCTTTTTACCCCAGGGTGGTTATGGAAGTTATGAAATAATGATTGGCGCTACAGTAGGTAATGATTTCTACACAATTATAGCCAACAACCAGTTTAAGTACGAAACACCTTTAGATGATCTTGAACAAAATGACTTTTTTGAGGTAAATTATTTACCTGTTTACAGTACATACAACTTTAGTGATTTAACTCAGTAAGTATGATTTTTTCAATTAGTAAACGAAAGTTAATTTGCGGATTTTTGCTAGTTATTTTAACAATAGGTGGGGTTTTGGGTGGTGTTTATTTAGTTACTAAAAACAACAAGGATAACTACCAAAATGAAAGTAATTTCAACAATCAAGAACAAATTAGTAAAATCCCTAATTTCAAAGCTATTGGTCCTGAAACACAACGTATCTTAAGAGAAAGAAACTATCCTTTAGATGATAGTGGTTATTATGTTTATAAGTATGGTGAAATTAATAGGTATCTAAGAAATGAAAGTGAACTAGATGAATTAATTAACTATAGAGTGATGGTACCTTCACTTAAATTACATCACAAAAGGGTTAATTTTGATAAGGCATTTCTTGAAAGTAAATTAAGAAAATGGATTATCAAAGCAATTAAGCAACATAACTATTTCCAACACTTTGAAAATGAACCAAATTTAAGAGTTCAATATAACATGAATATTCCAGCACAAAAAATTGATGTTAATGCTGTTTGATCTTATAAAAAAGATAATGATGCTGCTACTGGTAAGCCAATCCGATATTGAGATCAATTTGAGCTTAAACTTAAATAATAAATCTTTATAATTTTTACTAGATTTACTAATGCAAACGCATGAAATTCTTTTAAAAATTAAAGAAATTGCTAAATCAAAAAACTTTAATCTTAATTTAGATGAAAAGACAATAAATCAACCACTTCGTGAGTTGAAAATCGATTCACTTGATATGTTTAGTATTGTTGTTAGTCTAGAAAATGAATTTGGGATTAGTTTTGATGATGAAAAGTTAATGAATCTAAAAAATCTTGCTGACTTGGTTTTAGAAGTTAAAAACCTTTTAGCAAAAAAAGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCATAAAATATAAAAGCTTAGTAAGTTTTTACTAAGCTTTTTTCAATCCCCCCATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTATTGGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCTTTTGATCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATACAGGAAAGATGGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTGGTGGGGCTACATCTCCTCGCAAAGCCCTCACCATTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCCAAAAAGCCACTGAAACATAAGAACAGTAGTGGGGAGGTGAAGTTGGATGCGAGTGGGGAGTTTACTGAGGCCTGAAAACCATTGTTGACTACTGATCAAATAGCAAGAGAGAAGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAACAACAAACCCTTCTCCAACTCCCACACTGCTTCCCTTTCTTCTTCTTCTTCTTCCCCACTCCCCACTTTTTCTAACATCAATGTCGGGGTTAAATCAATGATCACTCATCTCAACAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACGGGAGCAGGTTATAGAAAAGCTAATAACAACAATAACGGCATCTCCTTGACAAGTGTGTTGCCTAGTAGCAATAGTAGTCAACAGTTTAATCCCAATTCAGATGATAATAAAGTCACTCAAGGTGGTGGCTCCCCAGCCAAAAAAACAACCACCTATTCCTTTTTACCCAATTCCATCAGTCCCACCAGTGACTGGATCAACGCATTGACTTTTACTAACAAAAATAACCCGCAGCGCAATCAACTGTTGCTCAGAAGCTTACTAGGAACTATCCCGGTATTGATCAATAAGAGTGGAACGGGAGATGAGTTTACCCATACGAGTGAGCAGAAATGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTTATCAACTAAATAAAAAACTTATTAGCTTATTTTTATTAGGTTTTTACTTATTTAATAGTTAAAAAAGTTTTGAATTTTTCTTAGTTTTTTATTTGTTTAATAGTTAAAAAACACTAGGCTTTACCTTTATTTAATTAATAAAACCTTTACCCCTATTACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTAGGGATGTAAAAGTTAAGTATGACAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAGTCAAGCTAACAAACCCGCCTACATCGTTGAATTTACTAATTCCACCAACATTGGCATCAAGTGAAGGGTAGTGAAAAAATATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACGAAGTACTGAAAAATTTAATTCTTGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCTAAAGAGAAGGGTAAAACGCAAAGGGAGGTGCATCTGGGTAGTGGGCAAGCAAATCAGTGACGATCGATGCGTAATCAACATGGCCTAAACAACAATCCCAGTCCCAATGCATCAACCGGATTTAAACTCACTACCGGCAACGCATATAGAAAATTAAATGAGTCCTGACCAATTTATCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACAGAAGCAACAACGGCAAAAAATGATGCGCCCAGTGTTTCTGGAGGGGGATCAGACACCACTTCAAAATTTAAAAGTTACCTCAACACCAAGCAAGCGTTAGAGAGCATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTACCCAGCTCTATTATGCTTCTACTAGCAAGCTAGCAGTCACCAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGAGCGGAGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAATACCAATTTAAACTGAGGGGAAGATAAACAAAAACAATTTGTTGAGAACCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAGTTGATCTTCAGTGGCTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAGGGATCTAGTGACCAACGACACGGGGCTAAATGGTCCGATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATACAAATTCAAGTGGTTCATCAGGAACCATTAAAACGGCTTATCCGGTGAAAAACACAGAAAAATCAACTGTCAAGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATGAGGGGATTGGGGTTAGAAATATTTTTATGAGTTTAAAACAAGGTAATGCAGATAACTAGCTAAATCAACAACTGGAGGTTTTGTAGCCCCATTTCTTAAATCCAAATTATGTAAGTTTTGCTGTTTAAGTAAATCCATAAATTTACCATCTTGAACTTTTATCTGTTCTTTTACGTGTAAAGTGAAAAGTAGTTGATTGTCTTTAAATTGAAATAGAGAACCTGGATAATCAGTATTTTGAACTAAGTTATAATCCAAATCACCATCAACTATGAACTTGACATCCATTTCACCATTTCATGATGAAGGGATACCAGAAAAAGGTGCAAATCAACTAAATAACCCAGTTAATTTGTATCCACCTTGGTACTTTATGTCAATTTCAAATTTAAATTTGTATGGATAATGTTGCCTAAATTCAATTCCATTATTTTTTCAATCAATGGTTGGATTATTTTCCTTGTTTTCAATTTTTATGCTTGTTAAGTTTAGTTTTATGTCAGTATAGGAAGTTAGCTTTCCTCTTAATCAGTCAGACATATAAAGACCTTCAAGCCCTTGTCTAGGAAACTGAATTCCTTTTTCACTAAAGGCTTTCTTTGCATTTTCACTTTCATCTTTTTTATTATTTTTGTAATAATCTGACGTTGCTTTAGTAAAGCTATTAATTAAAAGTGAAAAAGTTTTATTTTTATCAGCAGCAAAAGAAGTTTTTAGAGCATCTATTAGTTGAGTCTTATCAGTAACATCTTTCTTTTGATTTTTTCAATAATCTTTAAATTCTTTAGCATTTTCAAATGATTTATTAAATTGTTCTTGTTGTTCTTTTAAGCGCTTAGCTTCCTCTTCTTTTCGCTTAAGTTCTTCTTCTTGTTTTCTTCTTTCCTCAGCTAAAATTCGTTGTTGTTCTTCATAATCAGCTTTAGCTTTTTCACGTTCTTTTTTGAATGGTTCAATTATTTCTTTTTCAAATTTTGTTTTAGCTAAATCTAGTTGTTCATCAATTTTTAAAACTGAATTAAAGATATTTGTATTGGAAAGAAAAAGTGAATCACTTACAGCAGTTTTAGCTATTGAAGTGAGATCATTTTGATTTTTATCAACAAGTTTATTAATTAGAGGTTTTCAAAACAATTGGGTATTTAGATCGTTACCATTAAAGCTATTTTGCAACTCTTGTAAATTCAAAAAATTAGAAGTTTGGGCTTTTAAATCAAATTGCAAGTTAACAGTGTTGTTACCACCTTCATAATCAACTTCAAAATCCATTTTGATTTCAAAGCTATAACGAATCTTATCTTTAATAACAATGTCATTTTTTTAACCAATTCAAGATTAAAGTCTTTTAATTGAATTTTCTTTAAGTTGTATTGATTAAATATTTCAACAATACTTTCGGTAATGTTTAAACCTTTTGGATGCAATTGTGGAAAGAAATTTTCATTAAGGAATTTAAAAAAATTCTGTGGAACTTTAGGCAATTTATCTTCATCATTAATTGGAAAAATTTTGTTAGTTTCATTAATGCTTGATTACAGCCTATTTAATAAATCAACTGTTTTTTTAATACCTGATTTAAAACCACTTAAAAGATGAAAAATATCTATAACATTAAAGTTCCTAGGATAAAATTCATTCTTAAATTTCTCTTGAAATAAGTCAAAGTTTTTATCATAATCTTTCTCTTTAGCAATATAGTTTTCTTCTTGCTCTGTTATAGCTGTTTGCTGTTGGAGTAGAATACTATTTTCTCTACTATTTGTTGTACTTGATTTTCTTAGACTAGGTTGACTTAAATTTGCAGCTGAGCCATTATAGATAGAAGGGACAATAAGTACACCAGCAGTTAAAGTTGATGCAAAAGAGACAAAGAGAAAAGTTTTAATTTTAAAACGAGATTTTTTGATAGCTTTCAAAGTTAATAATTAATATTGAAAATAATTAGCTAAAACTAAAAAAGTTACAAACGAAATTGAATTTGTTTAAAACTAAAACAGACAGGGAAAATTTAACATTATATTCTAACTCTGAATAGCAAAAAATATAAGAATAGCTTTAATTCAAAAAAGTAATTCGGATCTTAAAAAATATTTTTTAATAAAGATTAATTGCCAGCAACTTGAATCTAAGTAATAAAGTGAATATTTATTTTTATTAAAAACTTATATTCAAATTTGTAGTCTATTTTTAACTAATATAAAAAAGTTGAATATTTAAAAACAATTAATTTTGATAATTAATAATCCAGGATTAATAAGTGCTTTTCAAAAAATTTACTTGGGTCATTCCAAGTTTGTTTTTAACAATTATTTCAACATCTTTACTTATTAGTTGTGCAACTAAAAGCGATAACACCTTAATATTTAATATTTCACTTGATCATAACGCTGATACATCAATAGAAAAATTCTTTACTGTTTTTTCAAAAAAACTTAGTGGAAAATTGAATAAAAAAATTAATGTTAACTTTAATATAGTTGATGATTCCTTTACAAAAATTAACAATATTCAAGCTAATAAAGCAGATTTTGCTTTTGTTAATTCACAAGCTATTGCTTCAAATAATTGGTTTGGCTATACGCCATTGATACAAACTTTAACAACAGCTTTTAAAGAAGATTTGGAGCTTGATTATTATGAAGATGGTAATTTACAAAAAAAAGCTGAAAAAACGAATTTGCTTTTTCTAAGTCCACCTTACAAAGAATGAGATGATATCAAACAAAAATGAACTGGAAATCGTTATGACTTTCTTTATGAACCTTCGAAGTTAGTTTCTTTTTACAGATCAATGATTTTAATAACTGGTTCAGCTAGTGAAATTACAGCTATTAAAAAAGCGTGAAATGAAAAAAACTGAAATCAGTTTATGAAATTTGGAATTGGTCATGGACAAACAAATTCAGCTTCACGTTTTGAGCTACCTGATCTTTTATTTAGAAAACATTTTGCTAAAAATTATCCCGGATTACAAAATGCAATTAATTCTGATCCCGATAAATTTGCCGTAGTTAGAGGAAGAGAGATAGGTATAAATAAAAACATCAAGATTGTTTTTGATGATGCTAATTCATTTTCTTGAACACAAAATATTAAAGGTTCAAAAAGACCTTTTTACACTCCAATTGATCCTAACGATAGATTAGAAATTCTCACTTATAGTGATCCGCTTTTGTATGACATTGGTATTGTTAGCAACAATTTATCAAGGATATATCAAAAAGCTATTGGTGAAATTTTTATTGAGTTAGCACAATCAAGTGAAGATCTATATGGGCCTTCAATTGGTTATAACGGCTATAAAATGATTAATGATTTTGAGAAAGAAGTTGTTGAAATAATTGAAAAAACCTATGGAAAATAAACCAATTCTTTCTTTTGAAAAAGTATCAATAATATACAAAAAAGCTCCACTTTTGCAAAACATTAGTTTTAAAGTAATGGCAAAGGAAAATGTTTGCTTATTAGGTAAGTCTGGAGTTGGAAAATCGAGCCTTTTAAACAGTGTTACTAATACAAAAATAGTTAAAAGTGGGTTAGTTTATTTTGATGGTGTTGCTTCAAACAAAAAGGAATACAAAAAACTGAAAAAACAGTGCAGTTATCTGGATCAAATACCAAATTTAATTGACACTGATTATGTATATGAAGCAATTTTAAGATCTGCTAAACAAAAATTAACTTGATTACAAAAATTAATTTGTTTTGAACCTAAATGAATTAAAGATAAGATCTTAGCAATACTAAAAGAAGTTAATCTTAATGATTATGTTAGTTGTATTATTAAAGATCTTTCTGCTGGACAAAAACAAAGAGTTGAAATTGCTAAGCTTTTTTTTAAATCACCAAAGCTACTTTTAGTTGATGAACCAACCACAGGATTAGATCCTTTAACCGCTTCTAAAATAATGGATTTAATTACTGATTTTGTGAAAAGAGAAAAAATAACTTTGGTTTTTGTAACACATGATATAGATTTAGCACTGAAATATAGCACCAGAATTATAGCACTTAAAAATCATGCTTTAGTGTTAGATAGATTAACAGAAAAACTAACAAAAGAACAACTTTATAAAATTTATGATAACTAAGTTGTTTTTTCACCAAGTTGGCGATAATAAAAAACGCTTAATTTGGTATTGAAAACTATTAATAATAATTGCTGTTTTGGCTATTGTCATTTACAGTTGAATAGATAACTTTTCTAGCTTTAATCAGTTTGGTTTAAATGTTTTTATTAATAACATTACAAGCTTATTTACCCCCAATCTTAACCACGAATATACACTAGTAAGATTCCTAGCACAAACTGCTTTTTTTGTTACAGGAGGTAGCTTTTTAGGATTTATTTTTGCGATCCTATTTTCCTATTGAACTGCATTTAAAATTCAACCCTTTTACATTGCTCTACCGATCAGGTTAATAACTATAGTTTTAAGAGCATTTCCCGTACTTTTATTTGGTTTTTTATTTAGTAATTTATTTAATAAACAACTAGCAGCTACACTAACGATTAGCTGGTTTAGCTTTTTATGGAATACAAAATACATTACTACATTTTTTGAAAACAGCAATTTAAAGTATTTTTTTAACAAAAAAATTAGAGAAGGAAGTGGTTTTAAAGCTTTTTGAACTACTATTTTTCTTAGTGAAAATGAGCGACTATGGTTGTTTTTTCTTTATAGTTTAGAAGCAAATTTTCGTTGAACTACGCTTCTAAGTATATTTGGCATTGGTGGCATAGGACAACTAATTGTTGATCCTTTAAGTATAAGGGTGCAGTTTGATCTTGTATTAATTCCATTAGTTGTTTTAATAACCTTTCTAATTTTTATAGAAGTTGTTGTTTTTTTATTATCAAGTTTTGTTTTTGAGAAAAATAGTGAAGATTTAAGACCAATTTTAAAAACAACAGTTATTGAAAAACGAAAGTGAAAAAGAATTATATTTATTTTGTTTATTGTAGTACTAATTAGTCTTAGTTTAGCCAATTTAGTGACTATTGATTACAGAATTAACGATGCTGAATTTTTACAAGATTTTTTTAACCAGTTTTTTCAGTTAAAAAGTAATCTTTTTTCAAGTAATGATCCTAATATAAACCCAATTTTAATGTTAGTTAAACTTACAACTCAAGCTATTAGTTTAATTAGCTTGGTTGTAATCTTTTCTATTCTCTTTGGTTTTATATCATGTAATTTATTTAAAAAGAGATTTTCAATTAGTTTTAAGATCTTATTACTTTTTGTTAGAGTAGTTCCTAGTATTCTGTTGTTTAGGCTACTAGATCCTTTATTTCTAGAAGCAAAAACAACTATTATTTTAGTCTTACTAATTAATCATGGTTCAAGTTATGGCCAATTGATGTCAATTAACTTTAATAAGGCAAATCAAAATATCATCAATAACTATAAAAATCATGGTATGACAAAAGGTTTTATTTTATGAAACTATTTGTTAGTTGAAAATAAACCTAATTTAATAAACATTACCAGTGATGCTTATGATAGTGTAATTAGGGATTTAATTTTGTTTGGTAGTTTTGGCGGTTCAATTATTGGTAGTAGAATTACTAATTTTTTTGAAAGAGCTCAATTTGATAATCTAGGTTCTGTTACAATCCCATTAATGGTTTATCTAATTGCAATAGAAATAATTTTTCTTTCAGTTAGATTAACTAGAATTTCAGTTTTTAAGAACTACCTTTACTAATGATAGTTTTGAAAGAAGCGCTCTAATATTAAAACTGCAGCTAATGTATCTTTAGCCTTTTTAAAATCCTTATGTTTTAAATCCATTGTTATAAGCTTATCTTTAACAGCACTTGTAGTATTACTTTCATCAACCAAAATTATTGGCAAATTAAAACGTTTTTCTAAAAGTTGTTTAAATGATTTAATTGCCTTTTGAATATCAGAATAATAGTGAAATTTGGGAAAACCTATCACTATTTTTTCAAGTTCATACCCATCGTTTTTAATTCGCAAAAATAAGTTGTTAACAGCAGTTTTGAAATTATTTTTTACTTCAAAAACATGAAATGCAGAAGGATATTTATCTAGTGTATTGGCAATTGCTGTACCTATTTTTTTCAAGCCAAAATCAATTGCTAATATATATTTCACTATTTATTTTGAAACAATTCATTAAGCTTTTGAGGGGTAACATTATCCTGAAAAGAACCTCTAAATAACTTATCATTGCCTCCGCCCTTTAAATTAAAACTATTTCTTAATTTTTCAATAATGGTGGTAGTTTTATTTCCTATAACAATAAATGAATTACTTTCATTGAATTGATTAATTATCAAGAAATTTTTAGTTTGATTTTGATTGAAAACATCATGTAGTGTTTGTAGCAATAGTTTAGGTTCTACGTCATTAAAAGTGGCAATTACATAACTTTTATTTTCATCTACTAAGGATAATAATTGCTTTTTAATTGATAAAGCTAAAGCTTGCTGTGATACTTTATAGTTTTTTGTTTTTAACTGGTTAATATCATTTTTTAAAGCTAATAAAGTATCAGATGCATCTCTTAATTGGGTAATTTTTTCAGGTAAGATAAATTTATCTAGCCTTTGTTGCAATTCTTTTAACTCAACTTTAAAAATTGAACTATCAATCAAAGATAGAACTTTTTCAAGTTCTGATTTTAATTGGATTAATTTTTGATTTTCTGCTTTTAAATAGTTGTTAATAGTTTCATTACTGCTAATGATTTCAATCCTTCATCTTCCAGCTCCTAAAGAATAGAAATCAGTAATAAAACAATCTTCAATTGAAGCAGTGTTAGCTACATGAGTGCCACCACACAACTCAACACTATAATCACCAAAGCGGATCACTCTTAATATTTCATGTTGAGAATATTCCTCTTCAAAATAAGCAATTGCATTTAGTTTTTGACTCCCTTCAAAATCAGTAAAAATCTCTTTTGAGCTTATTTTTTGTTTAATCAAAGAGCGAATTTTATTTTCTACTTTCTCAAGTTCATTTCTTGTTAAATGACGATTCAAATTAAAGTCAATAGTTGCTTTTTGCGCAGATTTAAAAGCACCACTTTGTTTAATAAGTGGATCAATTTCTTTTTGTAAAGCTGCATGTAAAAGGTGTTCTAAACTATGGTTGTTAGCAGCAAGTTTTCTTCAAGTTTCATCATGTGATAAAGTTACTTGATCATTGAGTTTAAAACTACCTACTAAAAAGTAGTGGAAGTGTTGTTTATTAGGTCCTTTAAATACACCTTGAAAACTGATTTTTTGATCATTATTATTAGAATGATTAATGCAACTTCCTTCATCATATCTCTGTCCTCCAGAAGTAGCATATAAAACTGTTTGGTCAAAAACTACATAACCACTTTGATTATTAAGTTCTTTAACTGGTAAATAATTTTCATCAAAAAGACCAATTACCTTAGCATTAATTTTATTTTTGTGATAAAAAAAAGTACTTTTAGTTTTGAAATTAACTAAATTAATATTTTGTTTTTCAAAATTTATAGTTTGGTTATTTTGCTTAGAGATAGAACGATGTTTGGCCATTAACTGATCAAATACTGTTCAATCAATAGTTAAACCTTTTTGATTAACTAGTTCTCTTATTATTTCAACAGGAAAACCGTATGTTTCGTTCAATTGAAATGTTAATTGGGGAGTTAGAGTATTGTTTTTAACACTTTTTTCAAACAGCACTAAACCTAAATCAATCGTTTTATTAAATGCATTAATCTCTTTTAAAACTACCTGTTTAACAGTTTCATTTTTAGCTTTTAAATGTTGATAATAATTCTCATATGAAGCGATGATTTCATCTATTATCTTTTCAATAAATGCTAAGTTTAATTGCAATTTCTTACAAGCTATTAAAGCACGTCTTAAAAGTCTTCTTACTACATAATTTCTCTCATTAGGACCAGGTAAAACTCCTTCTGAAATGGTAAAAGTGATTGCTTTAAAGTGATCAGCAATAATCCGAAAATAACTCTGTTCTTTCACTTTTTGAGGATCGAATGTAAAGTAAGAGTTGGGATCATATTTAAATGGACAAAAAGCTTCAATTATTTTGATTAGCTTTAAAAAGATGTCAGTATCAAAATTGGTTGGACTATTTTGTAATACTGAAACAAGTCTTTCTATTCCAGCACCTGTATCAATATTTTTTTGAGCAAGTTCTGTATAGTTGCCATTACCATCATTATTAAATTGACTAAAAACAATGTTTCATATCTCAACATAACGATCATTTTCAATGTCCTCAAAGAAAAGTTTTTCACCAATTTTTTTAGGATCAAATTTCTCACCACGATCATAATAGATTTCAGTGCAAGGTCCACAAGGTCCTAAACCTAAGTCTCAAAAGTTACGACTTTTGTCACACTTAATAATGTGATTTTTATCAATTTTATGCTTAATTCATCTTTTATAAGTAGTTTCATCATCTTCATAAACAGTGATATAAAAACGCTTAGGATCTAGCTGATAATAATTAACTAAAAGATCAAAAGCAAAATCAATTGCTTCTGTTTTAAAATAATCACCGATTGAAAAATTTCCAAGCATCTCAAACAAAGTTTGATGTCTTGAAGTAAAACCCACATTTTCAATATCATTTACCCTTAAACATATCTGTGCATTAACAAGGCGTTTAGATGGTGGTGTTTTTCTAGCACTGAAATAATCTTTTAAAGTAGCAACTCCTGAATTGATTCATAATAATGATGGGTCGTTGATCGGAATTAGTGATTTTGAAGCTAAAACCAGATGGTCTTTCTTTGCAAAATAATCTAATCAGGTTTGCCTTACTTTATCAGTTGTTCAATTCATTGCTTAATTTTAAATGCAATTTACCAGTGATAAAAATAACTATAGTTCTTTTATTTGCTAATTTATTTTTATTTCAAACTTTTTGTTAGCAATTTGTGCATAAACATGATTATCAGCTAAGAACTGCTGAAACTTATTTTGACTGTTTACTGTTCATAAATTTAAAGGTAAGTTAAGTTTTTTGATCATTTGGGGATACTTTTCATATATTTTGGTTCATGGGTGGAGGAATTGACAAATCTTTTGGATTCTAGCTGTACTAATTGTTTCAAACTGTTTTTTAGTCCAAAATAAAAAACCTTTTTTGTAACTATTATCTAAATCATAAACTTTTTGCAAAGATTCAAAGTTAAAGGATGAAAACAAGATCTTATCTATTGCTTTTTTACCATAACCTTTAACTAGGTCAACAAGCTTCTTTTCAATTCCTAAATATGGTTTTTGATCAGTTTTAATCTCAATATTGATTAACTTAAATTTATCTAAATAAAGATCTAAGAACTCTTTTAAAGTTAGGATCGATTGAAATTGAATTTTAAGGTGAAAAAAAGCACTATGATCATCTCTTTTTAAACTAACTAATGATTCAAACTCAACCTCCTTATTAACTAATGCGGTTCTCAATGTTGTCTCATCATGAATGATAACTAACTGTTCATCTTTAGTTAAATGAACATCAAGCTCTATTCCATCAAAACAATATTCAAAAGCTAAATCAAATGCTAGTTTGGTGTTTTCTGGAGCAATGAATGAATAACCCCTATGTGCTAAAAGCAATTGCTTGTTATGCATTATCAAGATCTCTTCAACGGTATGAAGCTTTATTTAATCGTTTAATTTCCATGCTATTTGAGATAAAAACAATTGATCCACATATCAAACCAAACAAAGCAATGGATAAACAAATAGCAGCGATAATTTGATAACCAGTGTTACTAGTATTACTTTGTCCTGCAACTGTATATTTCTTACCAACTTCAGCAGTCACAGTGTAAAGTCATCCATCAGTAGAAAAACCAATAAAACTAAGTAAACCAACACTAGATGCATAGTTATTTTTACCAATATCAATTTCACCTATTTGGTTATATCTGACTGTAACCATTCCCCATGATAAGATACCAGTAAAGATATAGAGAATGGAAGAGAAAGTAATTAAAGTGATGTTAGCAGAATTTGTTTGTACAAAACCAAGTAGAATAAATGCTAGAACAAATACAATACCAAGAACAGTACATATCATTAAAAACAAGATATAGCTTTTACACTTATCTGCTAATCTACAAAGATAAACACTAACAGCACTTCTCAGTGCATAAGTTCTAATTCCCCCAATTACAGTTACTAATACTACAGGCGCTAAAAATGCATTTTGCAACATCTGTAAAAGGTAATAAGCAAAAGTGCTTTGAAACACATACATCCCCATTAAAAAGAAGGATAGTAACCATAACTTTCAGTTTTTTAAGGTCACTAAAATCTGGTTTAAATTGCGTTTAAAACTTACTAAAGTGGTTTGACTTTGTTTTTCAATTGGTTTTTCCTTAACAAAAAACAAAACTGTAAATCCAGTAATTACTAGCATTATGGCAATGATAAAAGCGTATGCAGCAAATGGTTTTGAATCAGCATTCTCACTACCTCCTGATGGATAGAAAATGCTGGTAATTATCAATGCAATTAGAAAGATAAATATTAAACCTCATATTCCATTAGCAGCTCCCTGAATTCCAAAACCAAGTGCTTGGTTTTCTTTTGTTGCTTGTTGACTAGCTAGTTTTCATAATGGTGTTCAAAAGATTAAAGTACTTGTTATCCCTCATAACCCTCATATTACACAATACTGAATAAATAAAGCATCATGGCTTTGTTGGTTTTTAGTTAAGATGTTGGCTGCTAATCAAAAAGTAATTGCTCCAGTAGTAATTGCTGATAGAAATAACAATTTTCTAGAACTGAAGCGATTTGTTAAAAACCCTCCTGGTAGTTGAGTGGCAAGTGTTACATAACCAATGATAGAAGTAACAGTAGCAACTTCATCCTCTGTAATTCCTAAGTACAAGTGGAGGTTAGGAACAACATTCTTAACATAATAGGGTGCAGCAATTACAAATACATCAATTGCTCCCAAAATAATTAAGGCAAGAATTTGTTGTTTTGAAAAATCCTTTAAGCGTTTTTTAAATCCTTTTTTTGTTGTCACTATCACTAATGATAAAGTGGGATAAATTAAATATACATCTAATAATAAAAGTGGATGGAAGTGAAACGTTCTTCACTTACTTTTAAAAAGAAAAATAATGTTTACTAGAAAAACTATTTTTTCTTAAATTAATCCTGCTCCTAAACAAATTTGATCAAGTGTATATAATACACCAAACTGTCCAGATGCAACACTTATAACAGGATTTTTAAATGTTATTTCCAGTTTATTATCTGATAATAATTTCAACTTTGCAATTTCTGGTTTTTGAGCATGTCTAATTCTTACCAGAACTTGACTAGGAAGTTGCTTTGGTGTATACAACCAGTTAAATTGATCCAATAAAATTGTTGTTTTCAATAATTCTTCTTTGTCACAAGAAACAAATAATTCATTAGTTTCAATATCCTTAGCAACAACAAAATGACGTTGTTTTAACCCCCCTAAATTTAATCCACTGCGTTGACCAATCGTATAAAACCAAACACCATCATGTTCACTAATAGTTTGTTTGGTTTTTCAATCCTTAATTAATCCTTTTTTTACAGGTAAATAGTTTTTTAAAAAATCACTGAAATGTCTTTCACCAATAAAACAAATTCCAGTTGAATCTTTTTTATCTGCAACTTCTCAATTATTTTCTCTAGCAATATTTCTCACTGTTATTTTTTTTAAATCTGCTAAAGGAAAAATAACATTCTGAAATTGTTCTTTTTTAACATTTGCTAAAAAATAAGTTTGATCTTTATTGGTATCTTTAGGAATAGAAAGCAAAGGCTGATTTTCTATCATGTTTATTTTGGCATAATGACCAGTTGCAAAAAGAGAATTAGGGTTAATTTGCTTACAAAAATCATGCAATAAACCAAACTTAATAAAACGATTACACCAGATGTCTGGATTTGGGGTTAACCCTTTTTTGAAACTTTGAATCATAGGTAAAAAAACTTTGTTTCAATAAGCTTCAATTAAGTTTTTTTTTATTAACTTAATTCCTAAAGAATTAGCGATTTTTTTAGCCTGTTGGAAGTCTTGAAAAGATGAACAACCTGATTTGTTATTATTTATTTTCTTATGACCATAAAAATCATTATTAAGTGTCTCATCCCAACATTCCATAAAAACACCAATAACTTCTTGGTATTGCTTTTTTAAAAGTAAAGCACTAACAGCAGAATCAACACCACCGCTTAAACCTATAAAAACTGTTTTTGCAATAATTGACATTTTTAATAAACAGCGTGAATAGCAAACCTGTTTACTAATCTTTGGTGAAATCTATTAGGATTAATGCCTTTTGTAATAGCAAGTGCAGAAATAAACACTATTAATGCTTCATAAAAATTTTGACAGCACTGTTGATCACTATCTTTAGGATTTTCTACAACTTTTGCTAGATAATCTAGCTGTTTAATTACTTCAGTTTGGATAAAAAAACCATCACTTAACATTGGTTTGAAAAAAAATGGTTGGTCATCATAATCATCATCTTCATAAATAACAATATTGTCTTGTTCTCGTTTTAAACACAAATTAAAGTTAATGCGAAAAGTCTCAAAATCAACCGCTTGAAATTCAGTTTGTAAAAATTTTTTAAAAGCTATAAGTTGTGGTTTCATTACACCAGTCTAAAAAGTTTTAACACTTCTTGTTCAAGATAGCAATCCTTAAACAGTTTATTTCAGTATTACTTAAATAAACTACTAACCAAAATACTGTAAAAGGTTTAATTTAAAAACTAATTTTCTTTATTATCTAAGTTTAAGTTATTAAGTAAACCTAAAACATATTTTTCCAGATCAAAGATAGCTAAATCACTAGTTTTTTCACCAAAACCTATCAGTTTAACAGGCAGGTTAAACATATCTTTAATAGCTAAAATAATTCCACCCTTAGCAGAACCATCCATTTTAGTTAAAACAATCCCTGTTAGTTTGGAAAATTCATTAAATACCTTTGCTTGTGATAGTCCTGTTTGACCTACTGTACCATCTAAAACTAAAAGTGTTTCACTAGGTTCACTTCCACTTACCTTTTGAATAATTTGATAAATTTTTTGCAATTCATTCATTAAGTTAAGCTTGTTTTGCAATCTTCCTGATGTATCACATAAAACAAAGTCATATTTATCATCAATCCCTTTCTTTACGCCACGAAAGATAACAGCTGGAGTTTGTTCTTTAGGGTTTGGAAGTACAATGTCACAGTTTAACAGCTTTGCTCACTGATTAAGTTGTTCAATGGCTCCTGCTCTAAAAGTATCACCTGCAACAAGTAGAACACGTTTATTTTGTTTTATGAAAAAATCCGCTATCTTAGCTAAAGTAGTTGTTTTACCAACTCCATTAACACCAACAAAGAGATAAACATTTGTAAAGTTAGGTTTAACTATTAAATCAGTATCAAAGAGTTTATCTTGGATGTAATAAACAATAATTTGGTCAATAATTAGCTCTTTAATGAGCTGAAAATCTGTAATTCTGTTTAGCTTGATCTGTTCAATAATAGCATCACAAATTTTGTTTGCAGCATGATAACCAACATCAAGCAAAACTAGCCCTTCAAATAGATTTTCTTTAAACTGTTCGTCAACATTAACATATCGTTTTGACAGTTCATTAATAGTTTTAGCGAAAGTTGTAGCAGATTTTTTCAAACCCTGATAGTAAGTTTTATTATTGGTTTGAAAAAGGCTTTGTTTTTCAACTTCTTCTTTAAGCTGTTTAGCAACTGATTTTTTTGGTTTTAGTTTGGCAATTAACTTGCTTAAAAAGCCCATTATTAGTTAATTAGAATCATTTTCAGATACATATTTTTCAGCATTTTCAAGTTCAACTGCAAAGGTTTTAGTTACACCTTTAGTTTGCATTGCAGCACCCAATAACATGTCACATTTCATCATTGTACCTTGACGATGGGTAATAATTAAAAATTGGGTGTTTTTGCTAGCAGTTTTAATGATGTTAGCAAAGCGCTCCACATTAGCAGGATCAAGTGCACTTTCCGCTTCATCTAAAATAACAAGTGGAAAAGCACTTACTTTCAAAATACTAAATAAAACAGAAAGTGCAACTAAAGTTTTTTCCCCGCCTGATAACAACATTAAGTTGGCAATATTTTTACCAGGAGGATTTGCAAAGACATCTATCCCTGAAACCAATACATTACTAGGATCAGTGTAACGAATTTGGCAAGAACCACCACCAAATAGATATTTGAAAGTTTTTGGCAACTCTTGATTTAATTTTTGGATCAGTTGATCAAACTCATTGCTAGCAATTTCATCAATTTCAGTAATAGCTTTTTGCAAATTTTCAACTGCTTGTTGAGCTGATTCATATTCAGCGTTAATATCATCAAAGCGCTTTTGCTTTTCACTTATCTCAGCAATTGATTCCATGTTAATTACCCCCATTTCATTCAATTGATTTTGTAGTTTAGCAATCTTGTTATGGGCTTGCATAGAAGAGAGTTTAACTGGTTTGTTGTGATTAGCAATAGCAAACTCCATTGTCATTTTGTAAACACTGTTAATCTTTTCAGTGATATTTTGGATTGTATTTTCAAACCTAATTTTTCCTTCCCGCGCTGAAACTAATTTAGCACGTTGTTCATCTAACAATGCCCTAAGATCAACTATCTTCTCTTCACTTTGTTTAATTGTTTTTGCTAGTTGTAACTTAAGTTCTTGATTAAGCTTTAACTTAGAGTTAATTTCATCACGTTTAGCTCAAGCACTATTTAATGAATGGATTAATTCAGCTTCACTAGCAACAGCTTTTTTACCATCAAAAGTTGTGTTAGTAAGTTGTTCATATTCCATTTTGAGCTCTAAAATAATTTTTTTAATTTGAACAATACGCTCTATAAACTTGGCTTGTAACAACTCTTCATACTTGAGTTTTCTGTTCATTTCATCTAGCTTTACTTCTAGTTCTGTAAGCTCCTTTTTTAACTCTCGTTCGTTGTTTTCTAGTTTGTTAATGTTCTGTTCATTATCAAGTGAAGCTGATGATAAATACCCATCACTAAGATTTGTTTTTTCAAATCCACCATTGATAATTCCCCCTGCATAAACTGTCTCACCATCTAAAGTAACGATCCTATATAACTTGTAAGTATAGTTAGAAAGGTTAATGGCACTATTTAAATCTTTAGCAATAATAACTTGTGCTAAAAGAGTATTTACAACTGGTTGAAACAATGGATCACACTTAACATGATCACTACAAACACCAAGAAAACCATCAAGCTGCTTTAAAATTTCCATGTGTTCGTTTGTGATTTTAGTATCACTAGCAACATCATCTAAAGGTAAAAAAGTTACTTTACCTATCTCATTTTTGACTAAAAAATCTATTGCTTGGATGGCAGCATTATTGTTGTTAACAACTAGGTAACCAATTGATTTTCCAAGTGCTTTTAAGATAGCTTTTTCATACTGCTTATCAAATTTAAGAAATGTTCCTAGTGTATTTAAAATTCCTGTTAAAGCATTAGCATTTTTAACTAAGATATTGGCATTGTTAGTTTTTTTAAGCTCATTAGTTTGGAGTTCAATAATTGTTTTTAAGGAACGTTGGTAAATTAAATCAGCAGTATTTTTTTCAATCTGTAACTTTATCTGATCAACAAGTGATTTTTGTTCGTTAATTAGTTTTTCAAGATCAGTGATGGTTGTTTTGGAATTTGATAGTTGGTTTTCAAAACCATCAAGCTGGGTTTGATCAACTAAAATTAGTTTTTTTAAAGCTGCAGCTTTTTGCTTTTCATCTTTTTGTGAAAAACCTTGTCTTAACTGCACATCAATAATGACTTTACGTTGTTCCAATTCATTGATCTTTTGGTAAATATCTTGTAACTCTTTTTGCAATTCATTAGATTGCATATCAGCACTATGAAAGCGACTATTAAAGATAACAATCTGTTCTTCTAACAACTCTAGTTGGGGTTCGTGTATTTTAAAGTCATGTTCACTACTGTTAATCTGAAAATTAAACTTATCAAGTTCAGTTTGTGCTTGGAGATATTCCCCAACCAAAACAGCAAGTTCCAACTCTTTCAATTCGTTTTTAACCCTAATGAATTGTTGTGCTTTTTCAGCTTGTAATGTAAGCTTTTTTAAATCTTTTTTCAGTTCATTTAAAACAACGCTAACTTGTTTTAAGTTAATTAAGGTTCGATTTAACTGGTTAACAACTTCTTCTTTACGCTTGGTATATCTACCAATTCCCGACGCATCTTCAAAGATCTTTCTGCGTTCTTCTGGCTTAGCTTCTACAAATCAAGAGACACTCCCTTGTGAAATAATTCCAAGTGAACCTTTTTCAAGACCAATATCAGCAAAAATACCGCTAATTTCTTTTAAAGTTGCTGGATTGGAGTTAATAAAATATTCACTCTGGCCACTCCCTCTATAAACCCTACGCATCACGCTTATCTCTTTTCTTGAATCATGTAATAAACGGTTGGAATTATCAAAGGTTAGCTCTATTTCAGCTAACTTACTAGCAGGTTTGTCTTTAGAGCCAAAAAAGATCATATCATCACCTGATTTACTACGGAGATGTTTCATACTTCTCTCCCCTAAAACCCACTTTAATGCATCAACAACATTGGATTTGCCCGATCCATTAGGACCAACAATACCAGTCATTGAATGGGTAAAATCAATTGTGATTTCATCAGCATATGATTTAAAACCATATGCGCGAAAACGTTTTAGAAAAACCATTTGTTCTAAAAAGCTTTAATTTGGATGTTGTTATTAGATTATACTAGTTGAAGTAAACTAAACTAGGCTAATTATTAGGTTACTAAAAAATAAGCTAATAACGCTTCATTTAAAAAAGCTTCTATGTTTTAATAGTAACTAGTTTATTTAATTTAAAAATTACATTAATTGCTTTTAGAAAAGCAATATCATTTAAAAGTTTTTATTTAATTGCTTGAGCGGCAGTGATAATTCCAACATTAAAAATGTCACTGACACTAGCTCCCCTTGAAAGATCATTCACTGGACTTGAAAGTCCAAGAACAATAGGACCAATTGCATCATACCCTCCAAGTCTTTGGGCGATTTTATAAGCAATGTTACCAGCATCTAAATTAGGAAAAACATAGATATTAGCACTATTTTTTCAAGTTAGTTGAGGTGCTTTTTGTAACCTAACCTTTTCAACAAAAGCAGCATCAAACTGGAGCTCACCACAAACACTTTGATGCAATTCAGGGTGTTTTTCTAAAAATAGTTTAGTTGCTAAAACAACTTTATCCACCATTTCACCCTTACCACTGCCAAGCGTTGAATAGCTTAAAAAAGCCATTTTTATCTCATCCTCATTTAAACTTTTGGCAAAATTGAAGGTGTTTTCAGCAATTGTTGCTAACTCTTGGGAGTTAGGATAAACAGCAAAAGCACAATCAGTGAAGTACAAACGTTCTTCACCTTTTTCCATGATGAAAACACTAGAAACAAAATTACCAGTTGCTAGTAACTGTAAAGCTGGTCTTAAAGTATCTTTTGTAGCATATTCTTTACCACAAACCTCACCATCAACAACCTTTAGAGCAACTAAGGTAGCAGCTAAAGAACTAGGATCACGTACAAACTTTTGTGCTTCTTTTAAATCCATCCCCTTATGCTTACGTTTTTCATAGACAAAGTTAGCATAGCTAGTTAAATCCATCTCATCAATCACATAATGAGTTATTTTTTTATCAAAATTTGCAGGGATTTCCTGACGATTATGAAAGATAACTGCAGGTTGGATCAGCTTAGATTCATTAAGCATTTCAACTGCTTTTAAAACACTTGCTGATCAACCTTCTGGAAAGATAATTACAGGTTTTTTACTAACAGCTTGTAATCGTTTTTTAAAAATATCAATAACACTCATTGTTTTATTAATTTGGTTTAAGAATTTTTTTGAATATCGCTAATACCTACTAACTCTTCCCCACCAATTAGTGCTAAAGAAGCACCACCACCAGTGGAGATAAAACTAAACTGATCAGATAGTTGCATTTGCTTAACTGCTGCAGCTGAATCCCCACCACCAATAACGCTAAAAGCAGTTTTATTTTTAGCAATAATCTCACCGATTTTTGAAGTTCCTTTAGCAAAGTTAGTAAATTCAAAAACTCCAAGGGGACCGTTTCAAAAGATAGTTTTGGCTGTTTTTAAATAACTTTCAAATAAAGCAATTGTTTTAGATCCAACATCTAGAGACATATAGGATTGATATTGTTCTTGAATTTTGTCACTAACATCTAAAGTAATGCCAGTTTGATCTTTAAATTCAGAACCCATTACCTGATCAATTGCCAGCACAATCTTATTATGAGTATCTTTATCCAAGATTTGCTTAGCAACATCAATTAACTCTTTTTCAACTAGGGAATTAGCAGTAGCTTTGCCTTTTGCTTTAAGAAAGGTATTTACCATCCCTCCGCCAATTAAGATATTATCAGCAAGTTTTAGTAAGTTTTCAACTACCTTTAGTTTATCTGATACTTTCGCACCACCCAAAACAACAACAAAGGGTTTTTGTGGGCTTTGAATTAGGTAAGAGAGGTTCTTTAGTTCTTTTTCCATTAAAAACCCAATACAGGATTTTGCAACATACTTTGCAATTCCTGCATTAGAAGCATGTTTTCTATGGGCAGTACCAAATGCATCATTAACAAAAATTTCCCCTAAACTAGCCCAGAATTTCGCTAGTTCAGGATCATTTTTACTTTCTAATTTAACAATTTCTCCTTTATCGTTTACATCACAATAGCGAGTGTTTTCAAGGAGAAGGATTTCACCGAATGCTAATGCTTGCACTTTTTGTTTAACTTCAGCACCAGTGTTTTTACAAGAAAATTGAACCTTTACAGTTGGTAAGAGTTGTTGGAGTAATTCAGCAACCGGCTTTAAAGATTTTTTGTTGTTTAGTTTATCTTCTAAACTCTTAATCCTTGAAAGGTGTGATAGTAGCACTATCTTGCAGTTCTTTTTAACTAAGAACTTAATAGTATCCAAACCAGCTAAAATTCTTTCACTATCACTAATAACCCCATTGATCATTGGGACATTAAAATCACTTCTTAAAACAACGGTTTTGTTTTGAAAATCAATTGCTTGGAGTGTTTTGAAATTAAGCATATTAGAGCTTAGCACAATAGCTAACTACTCTCACTAGTTGGTGTACATAGGAAGATTCATTATCATACCATGCATACACCTTATAAAGTTTCATGCCATCAACTTCAACAATATTGGTTAGTTTAGAATCGAAAATTGAACCATATTCAGAACTTACCACATCGCTAGATACAATAGGATCTTCACAATATTTAAAAGAAGCGGAAGCAAATCGCTTCATGGCTTGATTTACTTGTTCAACAGATGGACTTTTTTCAAGTACAACACTTAACTCTACAATAGAACCAGTTAACACTGGAACACGGAGTGACATCCCATTAAGTTTGCCATTTGCTTCTGGAACAACAAGCCCAATTGCTTTAGCTGCTCCTGTTGTTGTTGGCACAATGTTAACAGCTGCAGCACGAGCACGACGTAAGTCATTATGAGGAGCATCTTGTAAGCGTTGATCTGCAGTATATGCATGAACTGTTAGCATCGTTCCATAAACAATCCCAAAGTTCTTTTCAAGTACATGAACTAATGGTGCTAAACAGTTAGTAGTACAGCTAGCTGCTGAGATGATCTTATCATCGCTACTAATGGTTTTGTGATTAACATTGTAAACAACTGTCCTGATAGTTTTTTCTTTAGCGGGTGCGGAAATAATTACTCTTTTAGCACCTGCTTTTAAATGGAGAGAAGCACCCTCTTCACTTACAAACCTACCAGTTGATTCAATTACTACATCAATATCATGTTCATCCCAAGGTAAATTTTGGGGATCTTTTTCACTAAAAACATAAACCTTTTTTCTATCAATTTGCAAGATGTTTTGTTTAACAGTAATCTTTCTTTTCAATTCACCATGAGCTGAATCATATTTCAACAGGTGCGCTAAAACTTCAGGTTGGGTCAAATCATTAATTGCTACAACTTCAACATTTGCCTTACTGAGAAGAGAACGAAAAACAAGTCTTCCAATTCTTCCAAAACCATTGATTGCAACCTTAATGGTTCTATTCTTTGCTGCCATACTTTAATTTAATAATTAGATTTTAATACTTTAGCCTATTGCAATTAATTCTATCTGTCAAAAATACAGCAAATTATTGCTATTAATTGCTCTTAAAAAAATAAAGTTTTGCTTATCAAAATTGAAACTATAATTAGGATTTTCTCAGTAAACTAAATTCCAGTTATTAGCAATTAATAAAATACTAAATAAAAGTAAACCCACTAATAAAAATACCCCGTTTAATAAGTTGTATTTTAAAGGATAATGAGTTCTGTTAGTGTTATTTAAATCATAACCTTTAGCTTGTAGAGCAAACGCTGTTGTTTCTGTTTTTTTAACTGTTGAAAGTAATACGGGAATAAAAAGGGTTTTAATCTTGAAAGGATTTAAAAACGAACACTTGTTATAGATAAAACCTCTAGTAGCTTGAGCTTGTTTAATTCTTATAATTTCACTTTTAACAGTTGGGAGTAATTTAAATATAACTGCAAGCAAGATACTAATTGGTTGAACCTTAATATGAAATAACTTAAGAAACTTAAAAAACCTCTCAACTGCTCAAGCCAATTCATAGATAGAACTTGAAGTTGTCAGTAAAAAAGAAGCACTGAAAAGCATACTAATCCTTAATGCAATTACAAAGGATCGTAAAAATGAACGTAGATTAAAACTTCACCAGCTAACACCACCAAAATTATTACCACCTGAATAGATAAAACTTCCTAAAAAATTATGGTTCTGATCAACGCTTAAAGCAGTATTTGGTAAAAAGATAAAACCGTTAACAATTACATTAAACCACAAGAAAAATAACATCCAACTCAAAATAATTAATGCACTTTTAACCCTTTTCTCACTAATTACAACTAGGGTTAAAAAAACTAAGTTAATAATTACAAGACCATATAATCCTAGCGGTAGAAAAGCAACAACTAAACTAATTAGTCAAAACCACAATTTTAGAAGTGGGTCAATTTTGTTTAAAAAACTGGTTTTTTGCATGAAAATAAGTTTTTTAGTTCACTAATAAGATCATCAAGGTTTTTAATTTCTGCTTGGTTTTTAAAAGCTATTCCCTTTTCATTAAACATCTTAACTGCTTGGATAATTACTGGAGGGGTGATTCCATATTGCATCAACCAACTGGTGTTTGAAAAAAAATCATTAACAGTGGTTTGATGAATTATCTTTCCCTGGTGGAGATGGATGATTTTATCAGCAGTTTCAAAGATAAAATCAACATCATGGCTAACAATAACAATCCTTGTTGTTTGTTTCATTGCTACTAGCATTTTGCTTAAGTTGCTAATCGCCATTTGATCTAACCCAACAGTAGGTTCATCAAGGATTAAAAACTTAACTTGCATTGCTAACACACTAGCTAATGCCAAACGCTTCTTCTGTCCATCGCTTAATTCAATGGGATTTTTTATTAACGGGATGTTTTCCAATCCACATAGATCAAGGTATTTTTTGGCATAATTAACATCTTTTTTATGACAAAGTTTAAGGTTCACTGCTCCTGTTAAAACTTCTTCTAAAATAGAATCACAAAAAAACTGATCTTCTGCTTTTTGCAAAATATAACCAATTTTTTGCTTAAAGTTTTTTGTTATCTTTTGTTGGTATTGGTTTGCAAAATAACAATAATTAGCACAATTTATATAGCCAAAACTAGGTTTTTCAAATAAACCTAACTGTTTTATTAGTGTAGTTTTACCACTACCACTTTCACCAAAAATAACTGTAATTTTATTAGGTTCTATTACCCCTGAACAACATTTAATGATCTGACCATCATTATGCTTATTGACAAAGACACTAATATTGTTAAAAAACAATGGATCTTGTTCAATGTTCAGTAATTTGCTATTAAAAAATGGAATGCTTTTTTTACTGTTTAAAAAGTAAAAAGGATTAATGATTTTATCAACCTTTTTATAAAAAGAAAACAGTTTTTTTGAATCTAATTTTGCTAGTTTAAGATTCGCTTTTTGAGCTTGAAGATGGTATTTTTGTTCAATTAAATCTAGTTTTATTTGAGGTGTTTGCATATCCAGTTAACTACATCATTCAATTCAAAACTTAAACTTGGTGTTTTTTTGTTTATTTGTTTAGCCACCTTATGGGCCAATAAAAGTGGAAAAGGGAGATTAAAGTGGTGGTTATGAAACAAATCAAGCTGCTCATATATCGCTTCAGGAGCAAACTTTTTAATTAGCGATCCTTTACTTAAAAAGATAATCTCATCTGCTAGAAACAGATCATTAAAATCATGGGTAATATTGATAACTATTTTGTTTTGTTTTTTGATGGTATCAAACACAAATTTTTTAAGTTTATTGCTGATCTTATTATCAAGCATACTAAATGCCTCATCAAGCAGATAAATTTGTGGTTTGACAGCCAATAAACAAGCAAAAACAGCACGTTGTTTTTCACCAAATGAAAGTTTTTTAAGTGGTGTAAATTGTTTATCTTTCAATTCAACAACAGTTAAAACTTCATTAATAATCTTATCCATTTCACTAGCTAAAACTCCATGGTTTTCCAAAGTAAAAATCAATTCCTGGTGGAGTGTATCAGCAAGTAATTGCACATCAGGGTCTTGTAATAAGATTCCAATCTTATTAAAACCAACAGAAGTCAACTCCTTATCATTGAAAAAGATAGTTCCTTTTTTAGCTTTTAGAAATCCGCCAAGGAGTTTAACTAGGGTTGATTTACCTGAACCGTTATGACCTATTACTGCTAAATGACACCCATCTTGAACATTAAAACTAATCTGATTTAACACATTGTTTTGGTGTTTTGGGTATTTAAAACTGAGGTTTTTAACTTGTAGCAAACTAAGCTTATTTATAAATTTTAAAAATAAGTCAGAGCTAAAAATCTGTTTTTTGGTAGATAAAAAACAAAAAAGCAGCTGTTGTGCTGCTCTTTTTTATTTGGAATATGTTCTATATGAACAGGTTATTTTGCTTGTGGACCTTGTTCACCAGTTGGGTTATATTTTTTTGCCATCTCTTCTCTTCACTTCTTAAAGTTTTCAATTTCCACTTTGAGTTTGGCATAGTCATTAGCTTTAATAGCAGCATCAATGTTACCTGTTAGCTTTTCTAACTTCTCTTTTTCTTCTTTAGGGAAATTCTTAGCATCAGGACTTGCTAATATCTCTTTGATGGTATTAACAATACCTTCCCCTTCATTACGTAATTCAATACGTTCACGGATGATGTTATCCCGTTCCTTGTTAGCTTCAGCATCACGGATCATCTTTTGGATCTCCTCCTCAGAAAGATTACCGTTGTCACTAATGGTAATACTGTTTTCCTTTTGCGTGGTTAAATCCTTAGCTTTAACATTTAAGATCCCATTGGCATCCAAACTAAAGGTAATCTCAATTTGGGGTTTACCTTTAGGTGCTGGTTGAATACCACCTAAGTTAAATCTTCCTAATGACTTATTATCTCTAGACATTGGTCTTTCCCCTTGACATACAACCACATCAACTGATTCTTGGTTATCTTGAGCAGTTGAAAAGATTTGACTTTTACTTACTGGGATAGTTGTATTTCTCTTAATTAAAGGAGTAGCCACACCACCTAAAGTTTCAATAGAAAGGGTTAATGGAGTTACATCTAAAAGTAAAACATCCTTAACATCACCACGTAAAACCCCACCTTGAATAGCAGCGCCAATAGCAACAACTTCATCAGGATTAATAGAACGGTTTGGTTTTTTACCTGGTACCATTGATTCAACTAGCTTTTGAACTGCAGGCATCCTTGTAGAACCACCAACTAAAAGAATTTCATTAATCTCTTCAGGTTTAATCTTAGCTTCCTTGATAACATCTGAAATAGGGTTTCTTGTTCTTTCAAGTAGTGGTTTTGTTAACTCCTCAAACTTAGCACGGGTTAGTTTTAACTCAACGTTAACAGGACCTTTTTGGGTAACAGTTAAAAATGGTAGAGAAATAATCGTTTCAAGTTGAGCGGAAAGTTCAATCTTAGCACGTTCAGCTGCTTCTTTAAGCCGTTGCATTGCCATCTTATCTTTTGATAAGTTTAAACCCTGGTGTTCTTTGGCAATGTAGGCTGAGATATATTCAATGATCTTGTTATCCCAATCATCACCTCCCAAACGGTTGTCCCCAGCAGTTGCAAGTACTTCAAAAGTACCTTCTGCAATGTCAAGTAAAGATACATCAAAAGTTCCACCACCCAAGTCATAAACCAAGACTTTCATCTCTCTTGATGCTTTATCAATCCCATAAGCTAAAGCAGCAGCAGTTGGTTCGTTAATGATCCTTTCAACATTTAAACCAGCAATCTTACCTGCGGTTTTAGTAGCGTTTCTTTCCGCATCATTAAAGTATGCAGGAACAGTAATAACTGCTCTTGAAATCTTTTTACCAATCTTTTTTTCAGCAAAGTCCTTAAGATAACTAAGGATTTGCGCTGAAACTTGTTCAGGACTTAATTCCTTAGTTGTACCATCAGCATTTTGGACTTTTACTTTATTTGAGGTACCCATCAACCTCTTGATGGAGACAATGGTATTTGGGTTTGTAACCATCTGTCTTTTAGCAGCATCACCTACTATAATTTCATTGTTTTTATAGGAAACAATGGAAGGTGTTGTTCTTTTACCTTCAGGATTTTCTAATACAACAGGTCTCCCACCTTCCATTACAGAAACACAAGAATTGGTAGTTCCAAGGTCAATGCCAATAATTAAACCATTGTCTGCACTCATACTTTTTTTTTAGATCAATAACAATTACTCAGTAATTATAAACAAAAATTAGCACTCAAACACTAAAAGTGCTAATTAAAGTTTGTTTTATTTATCTTTTTTAGTTTCTAAGCTAACAGTGTTAACTGTTTTCAACGGTACATTAACGGTCTTTTTAAATTCCCATATAACTTGAACAATTCACACAACAAAACAAATCCCAGTGAAAATAGCAGGTAGATAAACAGATAAAAGTCTTCATAAAAAAACACCATCTTTAATCTGATCATGGAGGAATTGATCAGTTGGTTTGAATGCATTGAGAAAACTAGTCATCACAAATTGTGTTGCACCTTCCCCACTAGCAACTGGAATAAAGTTAGAAGCGGTAACAGCAATATTAGTAATGTTAAAAAGATCAATTAAGCTGTATTGATCAATAACGTTATTAGTTGTGTTGACAGTTTTTGTGATCATAAAAACACCAAACAAACTAAAGTAGGAAACAATTGCTACAACCATGTTAGCTAGTAATTTAAAGATTGTTAACCCTCACCTTCGCATCTCAATTCCATACAACTTATTAAATTCAGCTTTTTCTATAAAACGTTGGTAGATCTGCTCTTTAGTTAAATAAGGACGTTTAAGTCACTTTCGAAACTGGTTTACAAGTGAATAGATCAAAACATGCATCTTTTTATTGAAAGCAATAACAATAAATAAGATAGCAACAACAACATCAAAGATCATCCCAGTAAGACTTAGTCAGTAAGAGACAAAGCTATTATGGTTATTTGCTAACAATTGATAGTTTTTAGAAAGAACAAAAAAAGAAGGTCAAGTTATTAACGCTTGCGATAGATTTCAAAATGCTCCTGTAGAAGTGACAATTAAAACTGCAGTTTGTTTTTTCATCCCCTTTTTAATGAATCAATACAAGCGAAATGGATCTTGTCCTAGTGATAAGGGGGTTACAATTTGAAAAAACTGTACTACAAAACCAAATAATAACCACTCTCACCAACTAGCATAAAAACAAAATCTCCTTGATACCCACCAATTAATGATTACATTCCACAAAACTGAAACTAAAAACCCTAAGATAACAACAAATATCCAACCCCAATTCTGATAGTTAATTGCAGTGATAATAGTTTTCACATCATCAACACTAACACCTAAAAAAAAGATAGTAACAATAACACTAATAACGATTAGAAAAACCAAAAAAAAGCTAAAAGCAACGATGTTTTTAGTGTTAAAAAAGGTATTGGTGGTTAACTTTGCCATTACTTAAGCAAGTTTAAGCCTGATTGAATTCTTTTATCACCAATCCTAAATTGGGGTTTTTCAATTGCACTTAAAGCAAGTTGTTGGGTGCCTGTATCACTAGCTTGAAATAAAACTAAAGCTGCTACTATATCACTTTCAATATTGGTAGAACTTGTTAAACTAAGTGAATCAATGTCACTTTTAGTGAACTGAAGATAGTTGTAACTATCACCACCAAAGTTAAATTCACTAAATTTTTGGTTGGTTTGGGTACTTCTTTTTGAAATACTTGGACTATTTGATGAATTAAAATAACTGGAATATTGGTCTAAAACAGCTAATAACAACGCTTGTTTAGCTTTGAAATCTAAAGCAGAATTGTTAATTGCATCGACCTGAAAATAAGCTGAAAGTGTTTTGCCATTACTACTACTACTACTTGGAGTTAGTCTACTTAGCTGTCGGGCAATGTTATCTAAAACTAATTGGTCTTTTGTTTCACTAATAAATTTTTTAAGATCATCCTTTGAACCATATTGTTTTAAGGAATTGGCAGCGATATGGTTTAAAAGTGCCTGGTTAGTATTGTTTTCTAACATGGAACTGTTAGTTGTATTCAAACCAGTAAAACCATAAGTACCATTATTACTTGTTGATACTGCTTGTCTGGTGCTTAACATCGTAGTAGCAGGTGTAGTGTTAGGACTTGTTTTGGTTTTAAAAAAGAAATTATTAGGATCAATAGTTTGGTTGGCATTAGCTGTTGTAGCATGTTCATCATTTTCATAGTTAAAAACAGAGCCAAATATTCCCTGAGGATTATTGGTGTTGTTACTGATAATATTCTTATCATAAGCAATATAGGCAATTTCACCATACTTTAAGTTAGCTTGTAAAATATCTTTAAAGTTTTTTAGGTTGTTTTCAATTAACCATTGCAAAGTAAAAATGAAGCGATAATGGTCAATAACTGCTTTAAAGTTAACATTAAAACCTGATTCATCAACACTCTCTAAAAAAGCTTTTTTAAACAATTCATCAATATTGCCATTATTAGGATTACCATACATGGCTTTATTGGTTAATTTGCTTCATGTCATGTAATAGTAATTGCTAATTACTTTAGCTAAACTATCTGCATCACTACTTGATGAAGTTGATGAAGATCCATTTTTAGATTTAATCTCTTCAAATAACTTTTTAGCTTTTTCAAACTGTTGATTGCTAGTAAAAGCATTTTTTAACAATGCAAAGGAAAGAGGGTTTACAGTTGTTACATCTAACAACGCATTTAAACTCCAATTGGCACTGTTAGTAAGTGATTGATCATTGTTAATATCAACTAGAATAATGTTGCTAAAGTTAGGTTTTGATTTTTCTGTAACTTTCAATTGGTTAACAAATTCTGTTACTTTGTTATTTATGTCGCTCAGAATTTCATCTCTTATCTTATCAACATCAGCTTTTGTAGTTTTTAACTTATTGTTGTTTTCACTAATATTTATTTTTTCACTACCTACTTTTTTCCAAAGTGTTTTAGTAATTAAAAACAAGAAATAGTTATTGAGTTTTTCATAATGACCAGACTTGCGAACATATGGCAAATGGGAAGATAAGCCATTTAACCAAGGGGTTGGATTAATTTGGTTAACATTGGTATTAAGTTCAGTTGTTCTACTTGTTAGTTTGTTATCTAATGCATCCATTTGTAAAAACAAATCTCTGATTACATTTGTTGCTAATGCAACTGGTGCAAATTTTTGCATTAGTTCTTTGTAATCATTGAATGCTTTTTGCATCGCATCACTACCTTTATCTTTTTTTTGAAAATCAGTATTTATTAGTGCGTTTACTAATATTAATTCCTGATTTTTTTCAAACCAAGTTTTCAGTGTTTCATTTAATGGAAAACTAAAGGAATATAAACTGTTTTTAAAGGTGTTTGTTTTGGCTTGAAAAGCTCTTCACATTAAAAACTGCTTTTGCTTTTCAATGTCTCTTTCAGTTTTGCTACTACTATTTTTTGTCAGATAATAACTACCTCCATCAATTCCAATTACAGCAACTCCCTCTCCGTTTCTAACTAGTCTTAGCTTCAATTGGTTATTAGAGTTATGGCTTGTATTTTGAACAGTAGCATTTGCACAGTTTGCACTAACAGTGCTAGCACCATTATTTGTTTTGTTATTAGGAATAGCTGCATCCCAAACAAGATAACTGCTTGTTGTTGATGAACTAGCTTTAGTTGAAACCATCATATCACTTGTAGTTGTTTGGTGGATTTGCTTTGCTAGTTTTCCTAAATTGGAACTATCATTAGAACTTATAAAAGCATCTTTAATAAGACTCTTTAAATTGTTTTGTGAACTGTCTGTTGTATTGTGTTCACAGAACAATGTATTACTCTTAGTATGGTTTTCAATTTTAGTTGATTCAACAACAATGGTATTGTTAAGTTCTTTATTAAGCTCTTTAATTGTGGGAAGTTGATCTTTATCTTGATTTAAAAGATCTAGTTTTAAATTTAAACTAGCACCTAAATTAGCTGCTTCTAGTTGATCAACAATGTTTAGACTATCTACTATTTGCTTGGTTTGAGTGTTATCAGAAAGAGTTGTTGGAAAATCAATCAGGTTGCCTTGATTTTGATCTAAGATGTATTTTTCTAAACCTGTTTTTGCTGTATTTAATCCGTCTTTTCCCTCAATTAACTGCTCTCAGCGTTTGTTGCCAACATTCTGGTTCTGAGGAATTTCCCCATCTTTAGGAAAGAAAGGAAACGCATAAGAAGGGGTTAATTTATCTTTTAGTTTTTCAGTGTTGTAAACTTGACCCAAACCTTGTTGTGGTGCTGCATATTTAAAAGTAGCTTGACTGATTAAAGTAGGATCGGTATATTCAACCCAGAGATTAAAGATTTGTGCTTGTAACTTTGCAAACAATGCATCATTAGTTTCACTAAAACGTGGTTCTTGAAAACCAAAGTCCTTTCATTTTTCAGGTTGGGATAGTTCATCATAGATATAAGGACCAGTTGAGAGTTGTCCATTAGGTTTATAAACTAAGAAATCCTTAGCGAATAATTTAGCGGTAAAATCACTAATAAGTTGATTGAAAAGTTGTTGGGATTTTCAACTAGCCTCACTGCCACCACTTTTATCTAATAGATCTTGTTGAATAAATACTGCTCTGTTTCTGGGTGGTGATATTAACCCATTAACAGTATTTTTATATTGATCATCAACATTCTTTTTAGTGTCTTTTAAAAACTTGTTTATGGCTTTGTCTTCATTGTTTTCATACCAAGCCTCAAGTGCTTTTAAAAGTGGTCCTATAACAAAATGTTTGTTTGCTGAAGGAGTTTTTAAAGCAGTAATAAGTCCTTCTTTTAAACTACCCTTTTGACCATGAAAAAAATTAGTTGAGGTTGGTGAAAAAACTGTTTGCAAAGCACTGTTTGTTGGAGTTGAACAAGCTACAAGTATTGTGCTTACTGACAAACCACAAAGTAAAAACCACCAGAACTTTGATTTTTTTAAAAGTTTTTTCATGATCTTGGTTTTTTCTTATTAATTAAAAGGTTTTGAATCTGTTGTTTTTGGTATTTTTTGTAGTTAGGTTTAACCTTTTTATATTTAGCTTTGATAGCTTTAATTTTCTTAATTTGTAAAGTTGAAAGCGGTTTTTTGGTAGTTTTTTGGTTATTTTCAGTCAAAAACTTCAAATCACCATTACTAGTTAAACTAACGTTTTGAAATTTAATTCCTTTTGCTATTAGTTTATTTAATTTACTTTGATTTTGGTCATTTCAAAAAAGATATGATCTGCCCCAACTGTTAAGTCGTGCTACACGACCAGAACGGTGGATGTAAAAACTATCAATTTTAGGTAGATCTCAAGAAATCACCACACTGAAATAATTTAAATCTATCCCACGTGAAAATAGATCAGAAACAACTAGTAATTTCAATTTATTATTAGTTGCTTTTGTGAAATTATTTTTACGTTCTTGATAGGTTAAACTACCATAAATTGAACCAAAACTAATATTGTTATTTGAAAGCAATTGTGTTAATTGTTTTAAAGATTTTTGATTAGAACAGAAAACAATAATTTGCTGATTTTGGTGGTGTTTTAATAAGGCTAATAAACCAGAAAAACGGTTTTCAGTATTGAGATGAACAACAAAATGCTTAACTAAGGGGTGAATCCATTGCTTTGGATGGAGATTAATAACTTTAGTATTAATTACTTGTTTTTTAATTATTTGGAGTTGTTGATTGAATAAAGTAGCACTAAAAAAGGCAAACAAGGGCTTTGCTTTTTGAAAAAGATGAACACATTCAAGTCACTGCTGGATTGAACTTTGCTCTAAAAACATGTCAATTTCATCAAAAACAACGTATCTAACTTTGCTAGTAAAAAGGTATTTTTCGTTTAGTAAACTAACAATTAGTTGTGCTTTTGAACTAAATGAAGTGGCAAAAGTAAAGGTTTTAAAGTACTTTTTGATTTCAGTTAAGATGTTGATAATTTGTCATTGTAACTCTTTGGTTGGTACAAAAATAACTGCTTGAGGTTGATCTAAACTGGTGTTAATCTTATCTAATAGAGGTAAAAGATAAGCAAAGGTTTTGCCACTACCAGTTTCAGCAATACCAATGATATTTTGGAATGGTCATAATTTAAAAACTGCTTGCTGAATTTTGGTAAATTCAACAATGCGTTTCTTATCTAAAAACTGCCTAATAGAAGAGGAAAATTGCATCTTAAACTAGAATTTACGCACTCACTGTGAACTGATTGCTGAAAACAATCTGTTATCACCAACTCTAACTAAGCCATTTTTGGCATTTGCTATCAATGCATTAATAGCTTGGGTTTGATTATTAGGATTAAGCGCTTCAAGCGCAACTGTACTTAAAAATTCTTCAAGTGAAAGATCAAGTCTTTTTGAATTATTTGAATCTGTACTATTCAAACTATCACTACCAAGCATACTTACATCTTCAAGGTTAATCTGTTTTGCATAAACTGCATGATAGATCTGGTTATCTTGTAAAAAGTTATAACTGCTATAATTACTGGTTTTATTTGTACCTATATACCCTTCAAACCTACTAAACACATCATTAAAGTTACTATTATTAGTTTTACTTTGAGCTTGTTTTAATTGAACAGTTTGAGTATTGTTAAAGTTTGAATTAGCAAAATTCTTCAAATTAGTTTTTTGTTCTTGTAAGGATTTTGATCTATCAACACCAGTAACGTTAATATTAAGGTTACTTGTTAAATGGTTATAAAGTGCATCAAACTCAGCTTGGGTTGGGATGTTATCAATTGTTTCTATAAGTTTTGTTAGAGAACCAAAACTAAATAAAGCACCTTTGTAAATTGTTTCATTACTACCACTTGATTGAGTAGATTGTAATTGTGCTTGACCACCATTAGAACTTGATTTTTCAGATTGGGAAACAAAACTTGTAAAGAGGCGATTTCTAACTGCATCTGGTAAGTTGTTGGATGAAGTGTTGGTAACAATACCATGGAAACCAAACCTTCTTTGCTGGTTATTGGTTTGCTGTGCACTTTTTTGCATTAAAGTTAATGATGTACTGCTACTACCATTGTTCTTTGTATAGTAAAGACTTGAAATATCATTTGCAGCACTGTTAACTGTTGGGGTTTTGTTGTTGGTTCAATTATAAGCACTCGAACCAAATACATAGTTAGGATTTTGTTGTTCTTGCTTGGTAAAGTCTTGATTAGTTTGAGAAGCAACGTTGTTAGCCATTTTTACTAATACAGATTTAGAAGAACTTACACTCATTGCTTGACTAGCAGTATTATTTCTGTCTTTATCACTAGCTAAACTTCAAACCAAGAAACTATTTGTTGTTCTTGATAGTTTAGCTTGCAAGTTCTGTTTTAGGTTTTTAAGGTTATCTTTTAATAGCCATTGAACTGTATAAAGATAAGAATAGTAATTGATTAAGTCATTGCTATCCAAGCGTTCTTCTAAGTTTAAGCTGTTGTATAAACTATCTAAAACCCCATTAATTTTGGGTTTATTATCAGTTGAACTTTCATTAACACCATTACCAAAAATAGAAGGATTAGCTTGTTTTTGAAAAAGATCAAATAGGTAGGTATATTTAAAGATATTTCAGTTTTCATTATTGAGACTGTTTCAGTTTTTAAAAGTTAATTTATCTAAATCAATCGCTTGTTTAAACTGATCATCATTGACAAAATAATCTCGTCTAATGGTATTTTGTAACTCATCACTTGATAAAACAGCATTTAAAGCTAAATTAAGTGCTAGAGAAGTGTTTCTAGGATCAGATGAAAGTTTGGTATCAACAAGGATAATTTGTGAATATTGCGGTGAGGGAATTACCGAAACTTTTAGTTCATCAACATGCTTTTTAACTGCTGCTTCAACTTTCTTTTTATTCTCTTTTAACTTGTCAATTATCTTTTTTGAAAAAGGATCACTACTACTACTATTACTATTACTATTACTATTACTTGTATTTGAACTAACTTTATCAATGATGTCTTTATAGTAAATACCAAGTTCATTGTAGTGTCCTGTTTTACTATCTTGTTCATAAGGTAAAACAGCAGCTAAACCGTTCATCCATATTTGTTTATTTCGCTCATTATCAATGAAAGGTTGGTTACGTTCTGCTAGTTTTAAAGCAACTTTGTTTTCTGCAGTAGCAACTTCATTAAAAACAATTTGTTGATATTGACTTTCAACTAAATCCTTCAACTCGTTTTTAACAGTGATAATGCTATCACTAAACTTTTTAAACTGAGGGAAACTTAAAAAGTTACTTTCTTTGCTGTCTACTAATTTAAAAAGGGCATTAAATAAAAATAAAACTCTGTTCTTATCAAAGTGTTTTTTCACTTCATCAAACAACTTAAAGTCATAAGTAGTGTTTGTATCAATTAAGCCATATTTGGTTTGTAGTGCACGGAATAATAAGAATTGTTTTTGTTTATTTACATCACGTCCTGATTCAGAGAGATAATAACCACCACCATCAACACCCATTAGATGGATACCATCTTTACCACGTGCTAAGATTAAATCAGGTTGACTATTGTTATCTTTTTTAAGTTCTTTTAGATCAACGATTGCAGAATTATTACTACTATCACTGTTACTTGTACTAGTAGCATTACTGAACTTTTTTTGATATTCACCTTTAAAAACATCTGTTGATTTAGTAGTTTCATTTTCTTTAAAGATCTTAGCTAGATCTGTTTTATGAACAGTAGTTGTTTGTGAACTACTACTTGCACTTTTCATTGCAGCCTTAGCTGAATTATTTTCAACAAAGAAATTTTCAATGATTGATTTATCTTTTTCTAATTTAGTGTATTCAGTTTGTTCTGCACCTTTGGATTTTTTTTGTAAAGCTAAATAGCCCTGGATAAAAGCTGCTGAAAAGCTAGGATCAAAGGTATCAAACATATCACTAGCTTTTAACAACAATTTACCACCACTATCACTAGAAAACTTATTAGGGATGTCAATGGTTTTATTGTTAGTATTTACATAGCTTTGTAAATTACTAGCTAGGATATGAAAACCATTTGCTCCTTTTATGGAGTTATCTTGTTGGTTATATTTGTTAAATACTTGAAATTGATAGGAAGGGGTTGGAGATTTAATTAGATCAGGATTAAAATAGTTATCAAACCCATCATTAGGAGTTTCATTAGTAAATACTACTCTTGAAACTAAGTTAGGGTTTTCATTAGTAACGAACTGATCTAATAATTTATCTTGAATGGCAGCATAAACTGCATCATTATTGATCCTAAGTCTTTTATTTTTATCAACAAACTTAGCTTGTATTTTGATATTATTCCAATTACTTTGGTTTTCAATTAAACCTTTTAATGGAGTTGATAATACGCCAACACTTTTATCAACATATTCAACAAAGTTCTTTGTAAAAAGCTTATTGATAAAATCATCAACAATTTTGTTGTTAACATCACGGAGCTTTCAGTTGGCTTGATTTCCACCAGTGTTATCTAAAATATCAGTTTGTAACCTAACTAAATAATCACCCCGATACTGATTTCTTAAGTTTTGTTCTTGATTAACAAAACTATTGTCTGTATCAGTATTAAAAGTTCTTAAATTTCTCTTAATGTCATCATCAACATTTTCTTCATAAAAATTGCGCAATACTGGCGCTAGGCGCATCGTTAAATATTGCGTTAGTCCTTCTCTATTTTCAAGTGCTTTATAAAGTGTGGCATTAATACTACCATCATTTTTATCAGTAAAAGCACTACTGGGTCTAAAGAGATTTTCAATGGTTCTGGAGTTTGGTTGTGCACAGGCAACAGCAACAATTCCCAAACTTACAGCTATGCTTGAAAGCAGCAATGGTCATTTTACTCGGTGTAAAAAACGTTTCATTGCACAGTCAAAAATTTCAATAATTTTAAAATTTATATTTTTATTATTACCACATGCGACTTGAAATAGAAAACGGGCTTGAATTTGTCAATGATCCTGTGGTAAATGAACTTGGCAAGATCTGTTTTTTTCATCCTTTTACAGGTAATTTAACAAACAAACTTAGTTTCAGAAGTCATTTCAATAGATACAGTTTTTATGCCATTAACTACCCAGGGCATGGTAATAGTGTTATTAACAATCCAAAGCAACTTGAATTTAGTTACTGATTGGAAATTACAAAACAGTTTTTTGATAAACATAACTTAAAGGATGTAATCTTATTTGGTCATTCTATTGGCGGTGGTTTAGCAGTTGCTTTAACTAACTATTTAAGCAGTGATCAATATAAAGCAGTCTTGTTAGAAGCACCATTAAATCCTGCTATTGTTGAAACTCCTTTAAATATTGTTCAAAATCTCATTCCTGATCCTGATAGTGATTTTGCTGTTATTCAAAAGTGTCTTGTTTACAATATTGAGAAAAAACTTGGGGCAAACTTTAAAGAATATTGTGAAAGAGAAAAGCAAAAATCAATTCACCAAAACCAACGCCTTAAAGTGATGTTAGAACCTTCTACACTCAAACAAAACATAGTTTTAATCAATGCAGCTTTTTTAAAGTTAAATTGTCCTGCTTTGTGAATCCATGGTAAGCAGGATGGAATTATTAAGTACCTTCCATCCAAGGCTTATTATGAATCATTAAACAATAAGCAAATTCAATTTAAAGCCATAGAAGCAGCGGCTCATACCCCTTACTTTGAACAACCACAAAAGTTTTTAAGTTTAGTTAATGATTTCTTTCAATTAATAAGTTAAAGCAGTTTATTTAACTAAACGCTTGTATCACTCAACAACGTAAGATTCATTTATTCCAGCAGGTAGTTCGCTACGCTCTGGGAATCTTATATAAGTACCTTCAAATGCCTTTTTGTTGGTTTCCACAAATGATGAGATAACACTGTTTTCAATAAAATTTTTAACTAATGGGGATTTAGTTATTCTTGCTTTTAAACGGACTTTATCCCCGGGATTAATGATGATTGAAGGGGTGTCAACAGTTTGATCATTTAAAATCACATGACCGTGGTTTACCATTTGTCTTGCACTCTTTCTTGTTGGTGCAAAACCCATTCTGTAAACTATGTTATCCAAACGTGATTCTAAAACTCTAAATAAATTAACTGTTAAGTTACCTTTTTGTTTTAAAACAAAGCGAAATAACCTACGAAATTGTTTATCAGTAATACCATACATGTATTGCATCCTTTGTTTTTCTTGGAGTTGTTGGGCATAACCTGATAAAGTTGAGGAACGAAACCTATTTCCATGTTGACCGGGAATAGATTTACGTTTTTTTCCTTTGGAAAACTCTTTGTTGTTTTCAAGTAAAGAAAAACCTAAACGTCTTGATCGTTTGAAAATACTACCAGTATATTTCATGTGTTTTCGATTTTAAATTATCTTAATAATCAAGGCTAAAATCACCACTGCTTTCAAATAAGTCTTCATCTAATAATGAACTGAAATTGGAAGTACGATCAGGAACTAGTGTTTCTATATCCAAATCATTAACACTAAAAGCACTCATTGAATCCATTACTTTGGCATCAATGTCAACAAAATCATTGTTAAAACCTTCAAATTCAAAATCGATGTTACGATCCATGTTTTCATAGATAAAATCATCTAACTGTTCATTGGAGATGTTATCAAAAGTGTTGGAAAAATCTATTAAATCATCCATTAACTGATTAGGTTCTACTAACCCATTATCTGTTACACTATCAAAATTGCTTAAAAGTAAAGGATCTTTTTCTTTCTTTGCAAAGCGGAGCGTATTATCAAGCTTATTAATGTTGTTCATAATAGTTTCACAACAAGAGTTATAAACAGTTTTAAGTTGTTTTAAACTACCTTGAATGCTTTCTATCTTCTTTTCATTTTCAGTGATAGCACGTTGAACATGACGCATAATTTCTCCAGATTTTTGCAGTTGTTCATAACTATTACTTGGTTTGTAAGCAATAGCAAAATCACTAGCTAATGATCTGATCATTTCTTCTTTTTGAAGTGTTGTTAGATTCTTAGCGTTATTAAATTGAACGTTAAGGTTCTCATTTTGATCAATTAAACGGGAAAGTTCACTTTGTAATTCCTTTTTAATTATTAAGTAACGTTCACTAATTTGGTTCTTATAGTTGGTAACAAAATCATTGAGATGGATAAATGATTGTTTAACAGACATCTGATAATCATTCATTTGAAAGATTGCATCAAAGTTAATTGTAGGGTTTAATTCACCACGTTTCAATTCACTAATAATCTTGTAAAGATCTGATTTTTTATTGAAATTATCAAGTTCACTTTTATCTTCAACCGATTGCTTATTTTGAGTAATTGAGTGTTCTTCAACAGGTTCTTTTGCTTTGACAAAAACTTCAATTTTAGGTTGTTGGTCTTCGCTATTAACAGTATTGATAGTTAGTTGATCATCTTTTTTATCGAGTCTTACTGATTCACTAGTAACAATTGATTGATTATTTAAGCTATTAATTTGTTGCAATTGGACAGATAATGTTTTTGCAAGTGTTTTGGAATCTTCAGTTATGGAATTAATGGTAATTTGTTCTTGCTGACTTTCTTTATTATCTAAAGCTAAAGTTTCAACTGGTTTATATTCAATACGGTTGGATACAAAACTAATAGGTAAAGTTGTAGGGATTTGGGGTGAACTTTTTTTGGTAGTGATAGTTATCTTATTATCAGACTGAATATCAAATTTCTGTAAATTATTAGTGTAGTTACTAGTTTCAACTACATCTTGTTTATTGAAACTACTTTCTAAAACTGGCTCCTTAGTTATTTCAGGTTTGAATTGATCGTTAACTTCGCTATTTACTTGATCAAACTGCTCAACTGTTGGTTGAGTTTCAAATGAAGAAGAAAAAGTTGTTTGGTCTTGTTGATCTGTATCAATTGGTAATTGGTAACTATCAACTACTTGGGCTTGTTTACTTTCAAAATCAGTAGTTACTTCTTTTTCAGCAGTTGGTTGTATTTCCTCATTATTATCAAGATTAGCTTGAACTTGATCAAATCCAACTTGATAATTATTTTGTTCTTCAGCAACTGGTTTGAGCTCTTCATGGTGATCAAAGTTGTACTGAACATGATCATCTAAAGAAGGTTGGTCAAGGGTTGGTTGAGGTAAATCACTGTCAAAGCTGTAGCTATCAGTTGTTGATTCAGGTTGTTTTGCAAAATGATCATCTGAAGAAGGCTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTTTCATCAGTTGGTTGTTTTGCAAAATAATCATCTGAAGAAGGCTGGTCAAGAGTTGGTTTTAGCTCTTCATTTTGATAAATGTCGTATTGAACTTGATCAACTGGTTCAGATTGACTTTCAAAGTGTGCATCAGGTGCAGTTTCACTATTAAACTTAACAGGTTCAGACTCTAACGAATTATTGCTGTTTGATTGAGATAAATCACTCCCAAATTTATAGTCATGAATTGGTTCAGATTTACTTTCATAATGCACATCATCAGATGCAGTTTCACTATTACTATTAAGTTGAATGAATTCAGAATTTAATGAATCATTGTTAGTTGCATCTTTGTTGTTTTCAGAGTAAAGATTAGATTCAGAATTTAAGTCACTAGTTTCAACTACATCTTGTTTATTGAAACTACTTTCTAAAACTGGCTCCTTAGTTATTTCAGGTTTGAATTGATCGTTAACTTCGCTATTTACTTGATCAAACTGCTCAACTGTTGGTTGAGTTTCAAATGAAGAAGAAAAAGTTGTTTGGTCTTGTTGATCTGTATCAATTGGTAATTGGTAACTATCAACTACTTGGGCTTGTTTACTTTCAAAATCAGTAGTTACTTTTTTTTCAGCAGTTGGTTGTATTTCCTCATTATTATCAAGATTAGCTTGAACTTGATCAAATCCAACTTGATAATTATTTTGTTCTTCAGCAACTGGTTTGAGCTCTTCATGGTGATCAAAGTTGTACTGAACATGATCATCTAAAGAAGGTTGGTCAAGGGTTGGTTGAGGTAAATCACTGTCAAAGCTGTAGCTATCAGTTGTTGATTCAGGTTGTTTTGCAAAATGATCATCTGAAGAAGGTTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTTTCATCAGTTGGTTGTTTTGCAAAATAATCATCTGAAGAAGGCTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCGTTATCAAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCACTGTCAAAACCATAGTTTTCATCAGTTGGTTGAGATTCTTTAGTGGAGATCCACTGGTCATTTTCAAAGTAACCAGATCAAACCCACTCACCATTTTCATCATAAGAACCATAGTCAGGGTTACCAACATACTTTAAATAATCATTCTCTTCTTGCTTTTCATCAAAAACAAACGGATTCCCATTTTCATCAAAGAGTTGGGAGATATCATATTCAGTATCTCCATAAGGATCATAGTAAACCCCTGTTTCTTTGTTAAAAGCTATATAATAGCCATTACCATCAGCATCATATGCTACCTTAATATTAGGGTCTTCTTCAACAGTGGATTTACCATCATAAAGATCACCAAAAATGTTTTCAGGTTCGGTTTGGGTATAGTTTTTTTCTTCAAAAACCGATTGCTTGTTTTTAGCCATCAAGAAAAGTTATTTATGCCAGTTTAGTCAAGCTTGATTATAAAACTTATCGCTTCGCTCTAACTCTTCCATGAAAGCGTAAATAGTAATAAATAATTTTTTAAATTATTTTCCACAATCCCTATTAAAGGGTAAAACAAAGCTACATAGATAGGTAATAATAACGCTTGCACCAATACTCTTGGTAAAAGAAAGAAATAAACCCCATACAAAACAAAGTTATTACTTGGTCTGCCATTATTAATAAAAGTAATAAACTCAATGGCAGCAATTGTCCCCAAAAGGATAGAAACAACTACTGACATTAAAACCATTAGAACCAAGCTATAAATAGTTAAAAGTGCTTGTTCTTTATTTCTCTTTTTTACATAAACAAACCCTAAAAATCCTTCTGTTACTAAAAAGAAAATAACAAAGCTAACTAAAGTTATGATTTGTAAAACAACAACATTAACACTAAGATCAGCAGTTTGAAAATTTTTTGTTCCAATTTGCTGAAACTCATTATTAGTTAGATAAAGTAAAAGCAGTGTTACACTAACAACAAAAAAAGTTACTAATAGTGTTTGTAACACCCAAAAATCAACCTTGTAATTACTGGCATTTTTTCTAACTAGATAAATCCCTTTAACTATGCCAGCAAGAAAAGCAAAGAGTGGCTTTTGCAATGCAAACATCCAAAACCACACATAACCCTTTGTTAACCAATCAATAGTATCAGATAAAAATCCAAAAATAAAACCCCAAATAGGACCAAAGATCCATCCGAACAATGCAAAGGGAATCCTTAGAAAACTAATGCTTAATACATTAGTAACACTAATTGAAAAGATAGAAAAGATAAAGGTTAGTGCTAATAAAACACTAGCTCAAACTAACAGTTGTAAACTCTTTAAAAGTCTCAACGGATAGTAAGGAAACAAAAAGTTTCAAAACAGTTTTTTAGATTGAAGCTTGAATGTGAATTTTAACCAAAAATAGTAGGTGGGGGGATATCTAAAGTAGTTTTACTTTCATCACTAGTAATTGGTTTTGCCAATTCCTCTTGAAAGTACTTATTAAATATCTCATTAGTTTGTTCTAAGTTTAATTTAGGACTTAATGAAAAAGCAATTACACTAATGATGCTAAAAGGAACAATGCTAAAAACAAACAATCAGGCGATTCCTATCAGATAACTTGCAAATAATGGTGAAACACTAATTAGTTGGTTTTCACTAAATAAGCGGATGATTAAAGCTGCATTTCAAAAGCCACTTCTTACTATATTACCAGGGATACAATTTAAAAAGACAAAGATAAAGATAGTCCAAAAGATCCATTTTATTCTTACCTTATAACGGTAGTTATCATTAATTTTGGGAAACTCTTTGACCCCAAATTCACTACGTAAATACCGCATTGCTCTATTGGTTTGACCTCTAGTTGCAAAGCTATAGGGTTTACCCTGAAAAGCAAAGAAAAAAGAAGCACCACCAAAAGCCAGGAAGATCATGGTAATCAAGTTAGATGAACTTAAAGCAAAAAAGCGGTTTAGATTTGTTGTATCTGCATTACTTTGCTGAACAGTATTTTGGTCATTATTAACATTGAAAAGTGGACTGTTCTTATCAACAAAGTGGGTAAAAAAGACATTGACCATCACAACGTATGCTATCGAAATGAAAAAGATGGCAATCATAGAGATGAAAATCGCTAGTTGTGCTCATTTAATCCACTGACTCTTAAACTGAAACTCAAAGATCTTACCAACTAGTTTTTCAGAGGTAAACCTACCAAAGGCACGATCTCTGGCACGAACTTGGAGGGTTTGTTGCAAAATATTATTCTCAAGTCCTGAGTTTAGCTGTTGAGAATTGTTTATAGTTTGAAAACTTGGGTTATCTTTAACTGTTTTATTGTTTTCTTGTAACTCACTAACGGGTTTAACTTGAGTTTCAGCAAGTTTGGTAAAGCTATTCAACTCTTTATTAAACAAGTTTTTAACAGCAACATTAAGATAATTAGCTAAATCTAACGCATCAGTTAGATCAAACTTAGCAGCTTTAAGTTCTTTTTCACTTAATTGGTTTTGCCCTAATTGGATAAACTTATCATGAAAACTACCAAAAAGAAAGTGGTCTGGTTTTTTTGTTTTAACTCTTTTTTGTTTTTCCTCTAAAAAATAAAGTAATTTGAGTTGTGCCAAAATTGTTTTGGCAAAATCACTATCAGTTTTTATCCTTGTTCTTAAAGTTTCGTTATTAAAAACAATGTCAAGATTATTTTGTACCTTAGTTAGAATTTCACTAAACGTCATTTTGATAAGTAATTTTAGAGAGGTTAGTTAGTAAAAAAGCTTAAAGCAAAGTTTTTTAACAACGGAATGGCAAAGCCATCATTGTGCTTTAATTTAATATCCAATTTTTGAATTGCATTAATTATATTTACTAAAACTTTGCTAGTAAAGTTATGGTACTGACTTTGAATAGCAATTAAGCGAAACTGATTAACATTTAGATTAATGTTAGTTAGTTGCTTTGGATTACAACTTTTAACCATCAATGCTAATAACAACTCCCCACTAAAAAATTCTAAAAATTGGGTGGCAAAATTTGGTTTTGTACTTGCCAATTCATCAATATATTTAAATGCCTTTACAATTTGTGAATTTGTTAGAGCTTTGGTTAATTTGTAAATTTGAACAGGTTGATAATCACAAATAACTGTTTCAACTAATTTGTTATCTTTGATTTCATTTTTACCTAACAAGCTAAGTTTGTTAATTTCTTGGTAAATTACCCCCATATTTAAAGGTAAAGCATTCGCTAATCAATCAATTATTTCCAAATCAAGTTTTAAGTTAAGTTCTCTACAAACTTCACCAATTGCTTTATGCATTGATTTTCAATCCAATTTATCACAAAAAACAGTTGTGATTGATTTAATGGTTTTAATCCCACTAAAAGGATTGTCAGTATAAACAGTTAAAACTACATCAGTTGTTTTTAGTTTTTCTAAACACAAGTTTTCTTGTCTAGTTAAGCTTGTTTTTTCCAAAAAACTGCAATTATTGACAATAAACTTTTCATTATTAGAACCAAATAATGGTTGTGAAAAGAGATCATAAAGTTGCTTAAGATCTTTAAACCAAATAGTTTTATATGGAGAATTGCTTTTGATTTGACTTAATTTTTGGTGAATTAGCCCAATATCTTGACCATAAATTATTGTCATAATAAGCTACTGTAAAAATAGTATATAAAAGTCCCAATAAGCCATAATAAACAAAAAAGAAAATCAGATTAGGTTTAGGTATTTGTAGAAAAACTGTTACTTTTAATGATCATTCACTTAGCATTTTAAGTGGGAGATAAATACCAAAACTAGCTTGACCAAAAACACCAATAAAGGGTAAAATAATTCAACTAACACAAAAATAAAATAAGGCAATTGGGCTAAACAAAAGGTTAAACAAGACACTAAAAGCATTTAGTCTTGAATTTAAATACAAGCTTAAGGGGCTGATAACAATCAAGATTAAACTAGAACTGACAAGCGGTTTTAAAGCTTTTAATAACTTTAGTTTGTTGACAAATAACAATACAAAACAAGCTAGAAAAGAAAAATTAAAACCAAAGTTATTTAAAGCATGGTTACTAATAAGAATGATTAAAAGTGCAGTTAAACTTAAGTTATCTTCTGGTAACTGTTTTTTAAAAACCTGTTTTAACAGTGTTGAGATAAAAACTCTTAGTGCTGAAAAGGCAAATCCTACTAAAAACAGGTAAATCAATAAAACAGCAAAACCACTAAGTTTGTTTAAGTACCATCTTTTTCATAAAAACCGTTCCATTAAATTAAACAAGAAACTTAAGTGAAAACCACTGATAACAAACAAATGGACAATGTTTAGTTTTAAAGCATTTTGGTACAAATTGTTTTTAGTGGTTTCATTAATTAAAAATAGTTTTAAATACTGATTTAACTCACCTTTAGTGGTCTGATCAATTCAATTAATGAAAGGGGTACGAATAAAGTTATTTTTAACAATTTGATTTATCTTGACTGCTGGAACAAAATAACAAAAAGCAATAAAAAAACCAACAATTGTTAAGTTCAATAAACAAAACTTTCAGTCATACCAAAGACTAAAAAGCGTTATCAAAATGATCCAAATTGACAGTGCAACAAACTGTTCTTGTTGTACTAAAAAGAAGCCTGGGATAAAACTGAGTAAAACAAAAAAATAAAACAGCTTTGTTTGCATGCTGTTTTATTAATAGATTTATTTTGAACTTTTTAACAAATTAAAAGTCCTTAGATCAATATGATCTTGATGGATAACGTCTAAGTCCATCATAGCTAGAACTAAATGGTAAACTAGTTCTTCTAAAACTTGATAGTCTTGTTGGTGAATGGAATGATGAACGGTATGAACTGATATTAGTTAATCTTCATGGTCTTCTTGTTAAGGGATAATAACTACTTGGTGTTCTGCTAAAACGATAGTTAGAAATAGCATTATTTTGGAAATTAGTTGATCTTAATGGTGTGTAATTGTTGACTCTAGATTTTGGTGTGTAGTCAAAATCAAGAGAAAAACGATCACTAGTTAAACTTTGATTGGTATCGCTGTAAAGTGGATCACTGTATTTAGTTGCAACATACTCATTTTCAGGATAAAAACGGCTGAAATTGCTACTTTTTATAGTGTTATCACTCTTTAGAGCAATATCACTCATTATCCTTTGGTGGTTTAAAGCATTAATTTTCTCTAGAAGAGAAGCGTGGGAAGAGACACTAACAGTGATATCTGAATCAGTTGTGCGTTCAACTTGTCTAGGTTGAAAACCAGTGTCTAATTTTGATTGAAGTAAAGCTGCTGATCTTGGTACAACTTTAATTTGTAAACCTGAAGTTGGCAATGGTTGTTGGGGTTGATATTCAGTTTTTACTTCTGGTTTTCAGTGGTGAACTTCAACTTGAGGAGTTGATTCTACTTGAGGGATGTATTTTGTTTCAGGTTTTGGTTCAATTCTTGGTTCAACCATTTGAGCTTTCGGTTGAACTTCAACTTGAGGAATATGTTTTATTACTTCTGGTTTTGATTCAATTCTTGGTTCAATCCTTTGAGGTTTTAAATCAACAACAGGTTTTGGTTGAACTTCAACATGTGGTACATGTTTTACTTCAGGTTGTTTAACTTCTGGTACTGAATCAACAACAGGTTTCACTTCTGGTTTTGGTTGAATTTCAACATGTGATACATGTTTCACTTCTGGTTTTGGTTCAATTTTGGGTTCAACTATTTGAGGTTTCGGTTGAACTTCAACTTGAGGAATATGTTTTACTTCAGGTTGAACTAACTTTGCTGGTTTATTAACTAGTGGTGGTTTTAAAGAAGGGGAATTATTAAAACTATGGTTTTGAGATCCACTATCAACTTGAACGGGTTGTTGGTGGACTAAATCCTGATGGAGCTGTTGAGTAGGTGGCACTTCTTTTTCAACAAACTGGTCATCACCCAAGTTTTCTGTAGGTTGTTGGGGTTGATTTAAACTGTCATTTAATTCAAAGACATCAGGTTCACTTTCAGTTTCTAGAGTAGTGTGTTGAGCTGCTTTTGGTTGGTCATCAACTTCTATCTTAGGAAGACCTGCATCAATACTAGGATAATCTTGTTGGGTTGGTTGTTCTTGAGAAGTTTGAGTACTGCTTAAATAGTTAATTAAAGGGACCTTTTTCTCATCAGTATAGCTAGAAACATCCAAAACATTACCATTTTGGTCATAGAAAGTGTTGTTATCCTGATCATATTGAACAGTTACCAAGTTGTTCTTATCATCATAAGCATTTAAGCTAACAATGTTATTTGTAAAGCTAATAGGGTTATCTGAAGTTAATTCAAACAAAGGATAGTTACTAGCTAACTGCTTTCTTTTTTCTAAAGAGAAGATATTACCTGATTCAGGATCTTGGTATAGAGCATGAACCTTATTTTTATGATCACGGGCATAAACAACACTAACATCAGGATATCTTTTGTTAATCTTTTTTAAAAGCTTGTTATAGGCTTTGTTTATTTTAGCTTTCTGTTTATCGTTCATAAAAAATTACTAAATTAGGGTTTTAAACCGCCTTTTGGCATCAATTGTGATGGGTTAGGACGTTGTTGCATATTTGGATTAAACCCAGGGCGTTGTTGCATGTTTGGATTAAAGTTATTTGGTTGTGCAAATTGGTTAGGATGGGGTTGGTTAAATCCAGGGCGTTGCATGTTTGGGTTCATCCTTGGGTTGAAATTATTGTGTGGTTGGAACTGTTGGTTTGGTTGGTTAAAACCAGGGCGTTGTTGCATGTTTGGATTAAACCCAGGGCGCTGCATGTTTGGGTTCATCCTTGGGTTGAAATTATTGTGTGGTTGGAACTGTTGGTTTGGTTGGTTAAAACCAGGGCGTTGTTGCATGTTTGGATTAAATGCAGGTCTTAAAAGCGGAACACGCTGTTGAAACTGTGGTTGAAGGGGTTGTTGAACTTGGGGTTGGGAAGGCTGAGCTTGGGATTGTTGAGGATCAATTTCAACTGTTTGTTGTTCTTGTTGATCTGAGATTCTTTGTAATTGCTCAGCAATCTGTTCTTGGCGTTCCTTCTCTTCAAGTAAACGTTTTTCTTTGCGCTTTACAATAGGTAAACCTATCGCAAGTCCAAGGATAATAGCTAAAGCACTAAAACCAAATGAACCTGCTACTGTAGGGATAAATCAGGGCTGTTCAACAATTGATCTGCCCTGAATTACAGTTGTATTATCTTCTGTTTGATGAAAAACAACCCCTAGTTCACTACCATTAGTTTTCACAACAAGTGCAAATGCTAGTAACGATAAACTAACAATTAGGATTGTTGTAAAAAGTAAAGCAAGAACTATAAAAAGTTTTTTGTATCTCAAAAATCCATTTAACTCCATCTTTATCAAAACTTAATTAGTTCCTTTTTCTTCTTGATCTTGAATCATCATAATACTCTTCTTGTTCTTGCTTTTTCATGTTCCTTTTTCTTCTTTTAATTGAACGAGTGATTTTATTGAGAAAGAAGTTAACAATTAAAGTACCTATTAAACCACTAACAAATGCCATTCCTAGGGGAATTACATACTTAGTTAGATAAGGTGTAAACTGTTCAACAAATCCAGGCAACTTAGCATTTTTCAAAAAATCTACATATCCTGGGATAGCATAAAATGCACTTTTTTCAGTTGGAAAACGGTTATCCAAACCATTACTTGAACCTTCTAAAAGTTGTAATAAGCTACTTGTTTGAGCAGTTGCAGTTGCACCATTGTTTGAAGAGATGTTATCCAACGCTTCTTTTGAAAAGAACCTGTTGAAAACATCCATTGGTTGTGCTAACTCAAATGGGTTTAGAGAACCAAAGTAAAAGATAGCTAAGAAAAGATAAGCAAAGCCTACCAATACCAATAAAAGAAAGCAAAGCTTAAAGAGAAATCGAAACAAGCGCATTTGTATTAGTCAAAAATAAAACTACTCTGTGAATTTTAACTTTATTTAGTTAAGCAAATGGTTTATGTAATTAATTAAATTAACTATAGAGCTTTAGAAAGCTCTAAACTCTAGAAAAATTTTTAGTAATTAGGATGATTCTTAGGATGAAATAGACCAAATTCATGAAGCTGCTAATTAATCAAGCCCCAAAGAATAAAGCAGATCTTCAGCTCTGTCTTCTTGCCATTTCATAATCTTCACTTTCATAGTATTTAATCAAATCTTGTTCATTACGGATCTGTAAACTCACAAAATAAACTGAAATTAATGAAAAGATTCCCCCTACTATTAAAGATAAGCCAGGGATAATTCTATCAAGTAGGTTTGTTGTAGAAGCAAACACTGTAAAGTTCAAAATAAAGCTCAATAAGCTCGCTACAATACTAATTACAAAAGCCCATAATAAGATGTGAAACAAAGTTTGGGTTTGTTGATATCGCCTTTTTTGACTTGGTAGCGCACTTAATCCTCACACTGAACCAAACACCAAACTAGCACCTAAAAAAGCATAAACAATGTCATTAGCTTCAAAGTTAACTTGTTTTGAAAATGTCGTTGCTAAAGTTAATAAGATCCCAAGGGTTATCCCATAACTAATCACATCAGTAATGATTAAGGCCCAAAACCATGTCAAACTAGTGTTGGGATTCCTTAATTTAAAAGCAGTAACAAAATAAAGAATTAAAGAGACAAAACTAGTAATAACTGCAATAGTACTAAGTGTTCTAAAATCACTAGCTAAATCAATTAGTGTGCTTCTTGAAAGCAATTGTTGTACTGTTAATGCAACAAGAAAGATAAAAAAGATCCCAAAAGCAGCTACCATAAAAGTTAAACGGATTACAGAAAGCTGTTTTGTTTGTACAAACTGCTTTGCTGCTAAACCGTTTTGATCAATATAGCCTTTTGTTGAATTAATCATGACAATTTGACTTGTTAAATTTTAATTTGTCAATATAAAAAAACTAATAATTGCAAAATATATAGATAAGGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAAAACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCAGGGAGATTTACCCAAGTGGCTGAAGGGGGCGCTCTCGAAAAGCGTTAGGTGGTTATCCACGCGTGGGTTCAAATCCCACAATCTCCGCCAAATTCTTAACTGTATAAACAAAAAACACACCATTTTGGTGTGTTTTTTTATGTCCATAAGTTAGTTGAATTTACTCATCAATGTAACCTGAATCACGCATTGTAGCAATCCCTGAAGGGGCATTACGGAGTGAAAATCATGGTTTTAAAAGCGAAGGAATAGTTTCATTTAACCCAGGAGCGGAGACTGGTAGAACAGGAAAGTAACTATCTACTACAGTAATTAAGAACTTAGCATTATCAGTGTCCTGTTCAGCTCAAATGCTGTTAAATCGGTTAAGTTGAGCAGCGTTGTCATAAAGATCTTTCAATTCATAACCAATGTGGATATCTGATACATTTGCTGGTTTGTTGTCATCTATCTTGCTTCTTTTTGATAGATATTTATCAGGATCTTTAATTGCTTCTTTAACTTTATCAGCTTTCAATACCTTATTATCAACTAAAAATTTCAGTAAGCTTAATGCTTTTTTAGTGAATGCTAATGAAGCGGATGTACCGTTATTACCATTAGTACCAAAGTTCTTAATGACATAGGGATCATCTTTAAAAAGATCAGCACTTGTTATGCAACTACACCAGTCTTTTCCGTTAGTTGTTGTGTTATTTTGAAGTTTATTTTCAATTTGATTAAAGACATTGTTTTGGGATGCAGCTAACAATAAAGTTCTGACAATATGACTACCTGAATTGTAAGTAATTACTTCACCAATACCCTCACTGTTAAGCTGTGTAGCAGCATCAAGTCAGGTACCAACCCCATCATAATCAGGACTCCAATAAGAAAAACCAAGTACACTTGATAAACCTATGTATCATTCGTTTCTTGTTGGACCTTGTGCATCTCCTAAGATAATCTCAGGGATAATTAAATTTTCACCATTATCATTACCAAGTTCAGTTAAAGCTTGTTTTAAAGCGTTATAGTAATCAACTTGGTTAGAGGAGAGTGATGCATTTCCTAGTTTAATGTTGAAGTGTAATGGATTGTTACCAGTAAACCCAACTTTTTTTAATGCACTGATAAAACTTTGCTTGGTAACACTTACTTGTTTTGAAGAAACTTGACTAGTTTGACTACTGCTGCCACTAGTAGAAGCTGTTCTTACTTGAGCACCATTAGTTTTTGTAGAGCTATAGTAACTTAACTTAACAGTTGCAGTATCAAGAACATTCTTATCCTTTTGAAAAGTTCCAGTTTGTTCAGGGATTAAACCAAGTCTTTGGTTGTTGTTAATCTTGTAATAATAATCATTCTCAATTCCATCTGTATTTGTACCACTACTAGTTCCATTTGCTGGTTTTTCTTTAAAGATCCCAAAAGGAACAGTAGAATAGTTAACATCTCCTGAGTTGGGCAGATCAATGATTGCTAAGTTCTTTCAGTTGATCAAACCATTAATTCCAGCACGGATAGTCAACGCTTCTTCAGATTCACTGTTATATAAGAACTTAGCTTCTGTCTCAGTGATGTTTGCATTTAGTTGAACAGTACTGTCATTAGCAACATAAGGTTTACCTGAATAAGTTACTTGAGCACTTTGCGCAGTTTTTTGGATTTTTAATCACCTCAGATCCCCTGTACCATTAAACCTTGATCTTGCATCTTGTTGTTGGGAATAGGGAACAGCTGAAGCTGATAAGCCCCCAGCGATATAACTAGAATAGAACACTTCAGGAGTAGCTCCTGGTTGGAAATAACTAACTATAGCAGGGATAGGTTTTTCGTTTTCTTGTCTTGTTTTAGGAAGATTAGGGGTGATAATTGCATCATAAATCTGGTTGCGTTTAAAGACAATTTGTGCTTGGTTAAAACTCTCAACATAGTAAGGGCCAACTGATCAAGTGTCTTTTCAAGCATTAACACCCCCTCCATAGATACCATCAAAGTTAGTATTTGCTTGATCTAAAATCTTTCTGTTGTTATTTTGGTTATATTTAAGCGGTGAATCTTTACCTAGTTTAAGGGCTTTTACCTTAGGATGGGTGTGAGGAATTGGAAAGAAGAATTCCTTTGACATCATTGATAGAAAGAAAGGAAAAGGAGAAGTTAGATAAACATTAAACTTGTTATCATCTACACTTCTATAGTTTTCAACATCATAATTTTCATCAGTGATCTCAATACCTTTACCATTGCCATCACTAGAACCGTTGGTTTTATCCATACCAACGGTTTTTTCAACATCAAGACCCATTAGGTCAATGAAATAACCATTCCGGTTAAAACCTAAGTTAGAAGAGAGAATATAGGTCTCAAAACCCCTTTCAAAGTCTTTAGAGGAGAGTTTAACTGGTTGGTTGTTTTGTTTGACTTCTCTACCAGCATTATCAACCCAGCTCAAACTAGTGTTAATGGTAAATTCATATCTGGTTGCTTCTTGGGTTATCTTGTGATAGTTAGGATCTTCAGTAATAGACTTTGAACTATCACGATAAATTGTGCCTATTGCATCCCACTCATTGAACTTAGTGTAATCATTCTTACTAGATCCATCAGAAGAATCTGTTGAACCGTTTTTTTGTGCTCTACCTTTAAGGCCACTGTGTGTTGTCTTTTTATAGCCAGTAACTGATAAAGCAAGTTCTAACACTAATTTTTCTTTAATTTGGTCAGTAACATTAACCCCATCATCACGGTTTGTACCAGTAGCAACATAGTTAGTTAACCCTGCATAAGCATTACCAAAAAAAGATGAAGTGGGTGAAACGTTAAACATGCTCAATGGAACAGAGTTGTTCTTTAACAAATCTGCTGAAGAAGAGGTGAAAAACACCCCTTTGTTTAACTGTGAGGATGCACAAGCACTTAGGATTAGTGCTGCACTAACTGTCAGTGTAGAACCGAGCAACAAGTATCTTTTTTTTAATTTCATTGTGTTCTTTCATAAATAAATCGGTTAATTGATTGCTGATTAGTAAAAAAGAGTTAAAAAACAGCAATTTACCCTTAGTTAAAAACTAAAAGTAGTTTTATTCTAGTTTGCAATCACATTAGATTGCAAAATCAAAAACTAATGTTTTTATCAATTGCTCACTTTTTAAACAAGAAAATGCTTAATACAAAGTTAGCAAAACTAAAAACATGGTGCACCCGAAGGGACTTGAACCCCCACTTCTAGATGAAACTAGATCCTAAGTCTAGCGCGTCTACCATTCCGCCACGAGTGCATTATTGGTGCATCTTGAGGGGATCGAACCCACGACCCAATGATTAAGAGTCATTTGCTCTACCAACTGAGCTAAAGATGCAGATAGTGGTGCCGACTATAGGATTTGAACCTACGACCTATTGATTACAAGTCAATTGCTCTACCAACTGAGCTAAGTCGGCATGGTGGATTGTGAGGGGATCGAACCCCCGACCCTATGCTTGTAAGGCATATGCTCTCCCAGCTGAGCTAACAATCCATCACTTATGGTGCCGAATACAGGAATTGAACCTGTAGCCTATGCATTACGAGTGCATCGCTCTGCCCTTGAGCTAATTCGGCATTGGTGACGCGTACGGGATTCAAACCCGTGAATGCACGCGTGAAAGGCGTGTGTGTTAAGTCTCTTCACCAACGCGCCAAATAATGGCGGCCACAACAGGGATCGAACCTGTGACCAACCGGTTAACAGCCGGTTGCTCTACCGCTGAGCTATGTGGCCTGAGAATAAATTCTAGAATTTCTAGCCAATTTTAACAGCTTCCTTAGGATATGAATAACTATTTGCTTTGGGATGTTTTCTAACAAAAGTTAGCACAGAACTGGACACACCAACCTGTCCCATAACCATTAATAGACATAAAGCAAGGAAATTAAAGGTATTTCTATTTGGATCTAAAGCAATGTTAACAGTAGCTCCACTTGAAAGTCCAACTGTTCCAAAAGCACTAGTTGTTTCAAATAAAGCATCAATGAAACTAACTGGTTGTTCCATACTTAAAGGTAGAAGAACAGCTGTTAGTAAAACTGCAATTAAGCTTATGATTAGTACTAAAAAAGCATCTATTACTGTAGTTTGATCGATTGAACGCTTAAATGCTTTTACTTCCTTTTGACCTTTAAACTTAGCAACTAGAGCTAAAAAGATTACTGCTAGCGTAGTTGTTCTAATCCCCCCAGCAGTAGAAGAGGGACTAGCACCAATAAACATTGCCAATGCAATAATTATTTTTGTAGTTTGAATCTCACTAGCAACAGGGAAAACACTAAAACCTGCTGAACGGGATGAAATAACCATAAAAAAGAGCTGCATTACCCTACTTGCATTAGGGTTATTACCAAAAACTGACGCATGGATTGCTTGGTTAGATTGTGATTGCAATTGGGTATTTATTAAACTCTTTTTTTCATCACTAAAATTAACAATAGTGTTAGTTAAACTATCACTAGCAATAAATTCCACCATTAAAAGTAAGGTGAAAAAAAGCAGGATTACAACGATATTAGTAATTACTGTCAACTTGGTAAATAGACTAAATTGGTGTTTTGTGTGTCTACCATACTTAATCTTTTTTTTAATGGCTTCAAAGCCATCAAACAAACAAGGATAACCAATTCCCCCAAAGATAATTTGGCTAATAGTTAACCACTGAATAATAATACCAAGTCCATTTCTATAAGGAACAAAAGAACTACCCCCTATCAGATCTATCCCAGCATTATTAACTGCTGATAAGGAATGGAAAAAACCTGCTTGAAAAGCCTTATTAATATCATTAAAAGCTGCTATTGTTTGGTTTGAATCAACTACTAAAGCTTTTAATTGAGTTGAAACTTTTGCATGATCTGCAAACAAGTTAGCAGGTTCAAAGCCTGGGATGAAATAAAACAAAATACCATATAAAAATCCATAAATTAGTTCAACGATAAAAAGAAAGATGATAGATACTAAGATCATCTCACTAGTATTACCTAGCTTAGAACCACCTCGTTCTGATTGCAACATTAACTTTTCATAAAAACTGTATTGTTCCTTCTTGTGAAAGTTAAACAATCGTCATGCTAAAAAAGCAATAACAACAAATCCAATCCCTCCTAACTGGAGTAATACTGCTAAAACTATCTGGCCAAAGATACTGTATGTTTTTGATACAACAACAGTAGAAAGTCCTGTATCACTAAAAGCACTGGTTGATAAAAATAATGCATCTAAAAAGTTGTAATCAGTTTTCTGTTCAAATCTTTTTCCCTGTCAATCAATTCCATAACTAACCACTTTTTGGTAGTTATCTTGGAGTGCAATTGGCAAAAATAACAGCAAACTTCCAAACAAGATGCAATAGATATAAAAACAAAAAATTCGTTGGGTAATAGTCTCACCTCAGCCAATTTTTTTTAACCAAGTGGTGAGTTTTACCATCTGGTTTTGTCTTCTTGTCATTAAAAGAACGCTATCAAATATAATTTTACCAAGGTATGAAACGTGCAGATTTTTGCATTATTGGTTTAGGTAGATTTGGGATGCAGGTTGCACAATCACTAAAAGAAAACAACTTTAACTTACTTTTAATTGATCTTGATGATAAAAAAACTGACACCGCCTCGCAACAGTTTGATTATGTTATTTGTTGTGATGCTAGTAATCTAACTGCTTTAGAAGAGTTACAAATTGATGAATTTGCTGGGGTTATTGTTGGGGTTACCAACATAGAAGCGAGCATCATGATATGTGCTAATTTAAGGGAATTAGGACAAAAAAACATTATTGCTAAAGCCAAAAATGAAGTACATAAAAGGGTGTTAAGCACAATGGGAATTAGAGAAGCTTTGATCCCTGAAAAGATTGTTGGTAAAAATCTGGTTATCCGCTTAATCCATGGGCTTGAAAATGAAATTATCAACCTTGGTAATGAGATAATTTTCATCCGTTCAGCAGTTAACAACAAGGCTTTTTTTAACAAAAGGTTAGAAGAGATTAACTTTAGGCAAAACACCGATGCTAACATTATCTCCATCATGCGCAGTAATAAAACTGTTGTTTTTCCTTTAGGACCAAATACTGAGATCCAACCAGGGGATATTATCACTGCAGTTTGTCAACAAAAAAGTTTAAATAAGTACTTAAATTACATCAATCCTAAAACCAAAAATAAAAATTAAAAAAAGGATCATCATGATCCTTTTTTTAGATAGCATGCTGTCAATAAGCCATGTTCTGTTTTAAAGTCAGTTATTTATCTACAGCAATTGCTGTCATTACTTTAATTATTTGTTCTTAAAGCAACATTCCCTTACCAAAATTTAGGTTTCTTGCTTGTGGAGTTTACCGCGTTTCATACCTGGTTTTCACCAAGCTCGTCTCTGTGGCACTTTCAAAACATCATCATAGTATTAACCTTAGACTAGTTATGTCGTTATGGCTATCACATCCTAAATCTTATCGCTTTGATTTACACAAACACTACTTGCATTCCAGCAAGTGCAAGCATGGACTTTCCTCTACTTTAAATATATCTTTAAAGCAGCAACTAACCGACAGCGTCAAAATTATAATTTGGTGTTGGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCATTTATCAACTTTAAGTTGGCCTTACACTCCCCTAGTTGGGAGTTTTTATTTTGCCTTACTCTTTTTAAAGAGTTGTTTTAATTTGGTTTTGTTGTAATTAAAAGCAAAGATAGCTATAAAAAAGAAAGAATAAAAAACTAAGCTAATCGCTCAAGCGGTAGCAAATACACCAAAACTATTTAATTTAGAGAGATAAACACCATTGTTATTTTCAACATTAAAAAACAAGCTAAACCGCGTTGCTGATTGAATTTTTTGTGTATCTCTTTCAAAAAACTGTTGGTCTAATAACACCAAAAATAAAACCATTAGCAAGATCCATAAGAGATAAACAACAATACTGATAAAAAATCCAGTAGTATAGCCAATGCGCTCTCACTTAGTAGTCTGACTTTTAAAAAATAATGAAACAGTTCATCTAAAACTTAGAAAAACTAATAATGCTAAACTAATTAACCCAAAAACTAAGTTAGCAATTCCATATTGACTAATCTGATAAGTAATACTAGCTTGAATAATATCAGTTGGTTTTTCAACAGTTCAAAAAAGGTAAAACTGATTAAAGCCGTTAACTTCGTTAAAAGTGAATTGTTTAGTGTGAAAAACGCCAGCTAAAAACAAAACGTTGTTTAGAAAAAAGCAGAGAAAAAATAAAAAGTAAACAAAAGTATCAAGTAAGCCAAACCTGCTGTAAAACAGATAGTTACTGTGTTTGGAAACAACTTGATACTCAGCGACATTTATTTCACTGAGTTTATCAGATATCTTATTAATCCAACCACCACCACTATTTTTCTTTTTAGAAAGCACGTGGGATAGATTTAGATAATCAAACTGATTTTTCTTTTTTAACAAGTACCATATCTTCTATCCTAATTCCACCAAGGTTAGGAATATAGATCCCAGGTTCAATGGTTACAACCCCATTTTCACACAATAACTTGTTGTAAGATTGGGAAACATTTGGCATTTCATGGATATCAATACCAACGCCATGACCAGTACTATGCACAAAAAAGTCTTTAAACTCAGAGTTTTCAATAATATCGCGGCACACCTTATCAACTTGTGAACCTGTTAAAGTAGTGTTTACTGCATTTATACCAGCCAAATTAGCCTCTTCAACTTTTTTGTATGCAGATAATAACTTTGCACTTTTAGGTTTTTTACCAACTAAAAAGGTTCTTGTAATATCAGAGCAATAACCGTTGTAAATGGTGCCAAAATCACAAGTGATAAAATCACCTTCCTTAACTATTGTTTTGGTTGGTTTGTGATGTGGGTTAGCACCGTTTTTACCAGTAGCTACTATAGGATCAAATGAGTTTTTAGCCCCACCCTGCTTCACAAGCTCATTAGTAATCCATTGTGAAATGAAAAGCTCAGTCATTTTTGGTTTAATAAAACGCTTTAACTTAACTGCTACTTTTCTTGTAATATCAACTGCTTTTTCAATGGCTTGAATCTCACTAGGCAACTTTACTCTTCTAATCTCTTGGGCATTAATTACTGTGTATTGTTTACAAATAGCTTGAATTCAATCCTGATAGTTAAAGGTAAGATAGTCACCCTCAATTAAAAGGTGATTGATACCATTAGATTCACAAAAAGCTTTAACTTGTTTAAAACTAACAAATAATTCAACTTCAACAATAGGATTAATAAAGTTCCTTGCTGCTTCATAATACCTACCATCAATAAACAATTTTGCTTTATTGCTGGTGATGATTAACCACCCTGCACTACTTGGAAAATTAGTTAATCAAAAGCGGTTTTGATCAGAACCAATTAGGATAGCATCAGCTTTATTGGTTTTTAAAAGATCTTTAAGAACTGTAATTTTTTGTTGTAATTCACTAATCATTATTTACGTTTTACCTCTTTGTGCAATACTACCTTACGACAACGTGAACAAAACTTATTAAGTGCTAGTTTTTCTGGATTTTTCTTGACGTTTTTAAAGGTTAAATAATTAATCTCAGAACATTCATTACAACCTAGTCGTGTGCTTCTTTTAACAGCCATAATTAAATTATCTTAATTATTTATTGTCAATAAGTAAACTAATAGAAAAAGCATCAATGCCAGTGTGAACAATAATCACACTTGTAATGAAAGCATTTTCAATTTCATTTTGAAAGTTAATCTTATTATGCTCAATGAAATCAGTAATTATTTTTTTAATTTCATTAGCGTAATTTTTACAAAAAGAATAGCAAAAACCGATCCGTTTAATTTTGTAGTTATCACCAAACTTAGTTTTAACAAAACCAAATATCTTCTCAACCGCTTGACTAAAACTAAAAACTTTTGCTCCTAGAGTATTAACTCCCTTGTCAAACAAGATAATAGGTTTAACTCTTAGTAAAGTAGTAATGAACTTTTTCAAACCAGAGATTCTCCCACCTTTACGCATTTGCACTAGGTTTTTTAAAGTAACTGCAGAAAGGATATTTTGTTTATGTGATTCAACCTTTGCTTTAATTGTTTGATTATCACAACCCTTGTCAACCAATGCCTTTATATCTTCGACCAATCATTTTAGAGAAATAGCAATATCACTAGTTTCAAACACTAAAAATTCTTTGTCTTTATTTTGTTCACTAAGCTCTTTTGCTAATTGAACCAACATATCATAAGTACCACTTAAACCTTTACTTAAAGGCAGAAAAATAAAGCGATCATACTTAGTTTTAATCTCTTCAAAGATCTTAAGAAGATCACTTTGACGTGGTAAGGAAGTTGAGATGTTAAGTCCATGTGGGTTTTCTTTTAGAAGTTTATGAACATGATCATAATCAATTTCTATCCCATCACGAAAGCTTTTTTCACCATCAACAATTACTTGTAAAGGCAAGATGTAAACGCCGTTAATCTCCCCAGGTTTAATAGAAGCAGTTGAATCAGTGATGATAGCTGTTTTCTTCATGAATTAATCTCCTTAGTACCTTTGTTTCTTTAAATTATCTAGAAACTCTAGCACATAATCAAAAAAAAGTTTAGGCGCTGAGTCGTGTGGTGAATGACCAACACCATCAATAACCTTAAAAATAATCTTGTCACTTTTATTAGCTAGATAGTCTACAGAAGCTTTGGTTGGCGTGACAATATCATTAGCTCCTAAAATAACTAAGGTAGGTTTATTACCAATCATTTCATAAGCCCTTTCAAGTGAGTCATTACCATATTTAGCGTTTTGAACCATATCACTGTAAAGTGTTTTAAAGGTAGTTCTTTTTTTAAATGCATTGATAGCTATTTTCAAAAGTGATTTACGTTTTTCTTCATGTTCAACAAAATCCTTATGGTTGCTGTTATTACGCTTAAAAAAAGTATCTAAAATCCGCTTTTTATTAACTGAGAAAGAAGTTTGATTCATAGGAGCTACTAATATTAAAGCCTTAATTTTTAGTGGTATAACTTTGTTAACTAAAACAGCCACAGCACCTCCCATACTATGACCTATTAAAATGACGTTATTTAGTTTTTTTTGAACAATAAAATCACAAACCAAATCAACAAAGTGGTTTAGTTTTAATTGATCTGTATCTGTTGATTCATTGTCTCCATGACCGGGAAAATTAAAAGTAAAGAAAGGCCACTTTTTCTTTTTAAACAAGCTAAAAATACGACTAAAACTGGCATATTCGCTTCCAAATCCATGTAAAAAAATAAAAACGTTTTTTCTTTTTTTAGGTTTAAAGGCAAATATTGAGTTAAAAAGAGTATTTTTATTACTCGTTAGCATTGATTTTAGCTAAACGTTCTGCATATGCTTGGATGCGTTCTTTCTTAGAAAGTTTAGCGAGTTTATCATCCTTATTTTGGATAACAAAATCACGTTTTTTAATTCGTGGTTTTTTTATTTCAACTGGTTTATCTTCATCACTAGCTTCTTTGATCTCTTCAGTTTGATTAGCACTAATGTCATTGAAATATTGCAACTGATTTTGCAGTGCTTGGTTTTCTAAAAAAAGTTGTTCTTTTTCAATTTTTAATGTTTCTAGTTCATCAAGATGTTCATCACTGGTAACTGGAGTAAATTGAAGTTCTTTTTTAGCATTTTCAAGTGAATTTATTTGTTGTTTTAACTGATTATTTTCACTATCAGATGCACTTAAACTTTGCTTGGCTTTCTCCAATTCACTGTTTGTTTCTTCAAAAGCAGAGAGTAAATCTTGGTATTGTTCATTAGCATCATTCAATTTATTCTTTTTAAGCTTTTCAAAATCAGTTTTTAATTTGCCATAGCTTTCTTGTAAGATTTCATGATCAGCTTGGAGCTTTGCTAATAAGTTATTGCTATTCTCAGAGCCTGATAATTCATCTTCTAAATCATTAATTTTTTCATTAAGTGCTAAATTAACTTCTTCAAGCGTGGCTAATTTATCTTGAGCAACATTAAAAAGATTTTGAGAGTCTTTGAGTTGCTGTTGGAGATTTACTAACTGATTGGTATTTAAATTACTCTTCTCTTCATTCCATTCTTCTAACTCTGCAATTTTGTCTTGCAGTTGTTGATTTTGAATTGTTAAACCATTAAGTTTTTCATCGAGTGCTTTTTTCTGTTCTTCAAATGAATTTTTAGTGTTATTGAAGTCATTATCTTTAGACCTTGTTCACTCAATTTCATCTAATAAAACATCATTTTCATGTTTAAGTGCTGAAATTGTTTTTTGATAATCTAAGTTTAGCGCTTGTTGTTTCACTTGCAATTTATCAAATTTTGTTTCATTCTCATCAAAAAGTAGTTCATACTGATTTAAAAGATCATCATATTTTTCATTCTCATCATTAGATGTGTTGTCATGGAGTTTTTTCAGTTCATTTTTCAAAAAATCATTTTCTTGTTCAACTAGATCCAATCTTTGGTTTGCTTCTCTTGCTGTTAGTAACTGGTTTTGAAGTTCGTTTATTTGGTTGTTTAAAACTGGTAATAAAGCTAATTGTTGTTGAACATTACTGCTCTTATTTTTCAATGCATCTAATTGATCTGAAAGCAAAGCTTTTTCTTGTTTTAAATTCTCCAACTCTTCATTAACTTCATCACTTAAAGGATTAATAGCAACTGGTTTATTTTGCAGTTGCTCATATAAATCCCTATTTTCTTGCAAAAGATCATCCTGTAATTTCTTGGTATGTAAGTGCTTACTTTTTTCCTCAGATAACCTCCTTTTAAGATCATCGATTTCCTGTTCTAAATCAAATATTTGATCATTATTATTCTCTTTAAGATCATCGATTTGTTTTTTTAAATCAGCATTTTCCAATAAAAGCTCTTTGTTATTATAGTCATCCAATTCATCAACTTTTTTTGTTGGATGTTGCTCTTTTAAGATCCTTTTAACAACAAAAGGTTGATCATCATTCTGATCATATTGGATCGTTTTATGAACTGGATTGATAGCTTTGGTTAAATTTTCAATGGAATAGAAATCATCAAATTGTTCAGGTTCTTTAACCTTATCAGTTGAAAATATTTGAACTTCTGGTTTTTTTGTTTCATTAATCTCTTCACTAGGTTGGTTAAACAACTCTTCAAAAGATACTTGTTTAGTAGCTTCTGGTTTTATCTCATCCTTATCATCTTCAGCAGTAATCACTGATGGTTTTAGTTCCTGAAAACCAGTTTTAATTTCCTTGGTTAAAACTGATTGATCATCAACTTCTTGAAAAGCCATAGTTTGATCAACTGTCTGTTGCTCTTCCTCTCTAGTTTTAAGTTGATTTGCATAATGATTTAAAACATTCTTATCCCATTTTTTATCTAAAACCTTTAAATCTATCATCCAATTAAAAAAAGAAACTATTGACTTTTCTTTCTCAGCTTCATCAACAGTACCAAAAAATGTTTTCTCCAATAAATTTCTTTCAAAGGGAATAGCAGTAATTTTAGATGGTGAAAAAAGGATATTATTAGTTCTTTGTACTAATTCTTTTAAAAGTGTTTTTTCATCAAGCTCATTATCAAAATCACTAATTTCAAGTTCCTTATCTACTGCCATGGATTATGTTGGAATATTAGATAAATAAGTATATTATCTTAAACTTCTGGCTTTGTTCTTATCCGTGCGTTTTTTGATTTAAAGTAAAGGCTAATAGGTACAGAATTAAAACCAAAGTTTTCTCTAATCTTATTCTCTAAAAAACGTGCATAAGAAAAATGAAGATACTTAGGGTCATTGCAAAATAAAACAAAATGGGGAATTTGACTTTTGGTTTGTACTGCGTAAGTTATCTGCAAACGCTTGCCTTTAAAAAGTGGAGGTTGATTGTAAAGTTGTGCTTGCTGAATAACATCATTTAAAAGTGGTGTTGCAACCTTAGTTTCAAGTTGGCTTTGAATAATTTTAAGTTGTTCAAAAATAGTATTTAAGCGCTGATTTTTCAAAACACTAATAAACAAAACAGGCGCAAAATCGAGGTGTTTAAAATGCAATTTCAGCATTTTTTTATATGCATTTGTGGTGTTATTGTTTTTTAAAACTAGATCCCATTTATTCACAAGAATAATAACAGGGATAAGTGCAGCTTGTGCCAATCCACCAATCACTTCATCTTGTTCACTAATAGGTTTTGATCCATCTACCATCAAAAGGATAACGTTACTACGGGCAATAGCTAGCTTTGTTTTGATGTAAGATGCGGTTTCAATTCCCATGTTAATTTTGCCTTTTCTTTTAATACCAGCAGTATCAATCAAAAGAAATTTTTCACCATTAACTTTTAAAGGAACATCAATAGCATCTCTAGTTGTACCACTCTCATTTGAAACTAAAACACGATTTTGTTTTACAAGTTGATTAATAAGTGAGCTTTTACCAACATTTGGTTTGCCAATTACACAAAACCTTATTTTTGCTAAATCATCATTATTTTCATTAGGGAGTAATTGATTTTGTTTAACTAAGAGATCCATTAAATCACCAATTCCAATTCCATGAGCAGCACTAATAACAACTGGTCTTCCAAACCCTAAGCTGTAATAATCTTTTAATGTTTCTTCAGCAGTTTTAGGGTTAAAATTTTCAGCTTTATTTACCACTAGTATTACTGGTTTATCCTTATTTTTTTTAAGAACCTTAGCTACATAAAAATCATCGCTATTTAGTTGTTCTTGTAGTGACACTAAAAAAATAATAGCTTTGGCTTGACTAAGTGCTGCTTGTACTTGTAATGCAATTAGTTGTTGCAAGGGAGTTTGTTTTGCAATTAATCCACCTGTGTCAATAAAAGCTATCTTTCTTTTTAACCATTCACCAATTCCAAAGATCCTATCTCTTGTCGTGTTAGGAGTATCTGAAACAATAGCCATTGGCTTTTGAATTAAGCGATTAAATAAAGTTGATTTACCAACGTTTGTACGACCTATTATTGCAACAGTAAACACAAATTAAAGTTTAAAAACCTTCTTTGCTTCTTTGAGGGTTTGTTTTACTACTGCATCAAAACTTAGTTCAGAAGTGTCAAGATAAATAGCGTCCTGGGCTTTTTTTAATGGGTCTGCAGTTCTAGAACTATCAATTTGATCACGTTGCTTTAATTCTTGAATTAGTTCCTTTAGTTTTTTTTCATTTGATAGAGAAATTCCCATATCTTGTAATCTTCGCTGCGCTCTAATTTCAACTTTAGCATCTAAAAAAAACTTCAATTGAGCATTTTTTAAAACTACTGTTCCTATGTCTCTACCATCCATCACTATGTTTTTATTTTCTGCTAGTTTCTGTTGTTTAATAACTGCAATTTTTCTAATGTTAGGATCAACAGCTATTTTACTAGCAATGTTAGCAACTGATTGGGTTGTAATAACTGTTGTAATATCAGCATTGTTATAATACACAGCATCTTTCTCAAAGCGTCAGTTAATTTGATTAATGATTTTTAAAAAAAGATCAATATTTAATCTATTCACTTGCATTACATAGGCAAAGGCACGATACATTTTTCCACTAGAAAAATAAAAAAAATCAAGTTCTTCAGCTATTTTTTTAGCAACACTGGACTTTCCAGAACTACTTGGACCATCAATTGCTATTTGTCAATTCATAACTAAATTTAAACAAATGAAACAAAACTAACAATCAAAAAAATAAGAAAAAACAAAATGCTAAACAGCATGAAACTTATTAAAGTTATCACTAAGTTCTTAAAAATAGCCTTATTAGCATTAAACAAATTAGGTTCGATGTTATCAATGCTTTTTTTAAAAGCAAAACTTTCAGTTGAAAAAACTGTTAGTATCTTGTTGTATTCTTTTAAATCACTAGTTAAATTGCTTGTTTTTTTTAATCATTGTTTCTCATTAGCTTCATAAAATTTACTTAAGTGGTTAATCTTTTCTATTCCCTTATCATCACTAATTCAGCTTTCATAACCGCTAGAAGTTTGGTTATATGCTGCGTTTAACCACATCCTATCAACTTTTTCTTCTAATAATTTAAAGTCAGTATTTTTTAAATCAACTATTAATTCCTGAAGTGTTTTAAACAGTTTATTTGGTTCAGTGTGATGTTTAACTGGATTTTTATTAAATTCACTAAAAGGATGGTAATTAATCAAGATTTTCTGATTAATTTTATTAAGCTGTTTTTTTCAGCTTTCAGTGTTCTTCTGAGCGTTAATTAAAGCTTTTTTAACTATCTTATTGTTATCAAAACTTTGTCTATAAAAGCGAAATTTTTCTACTCTTCCCATGGGTTGGCATTATGAACAACAAACTGAATGTCATCATCTTCCTCACAAGCATTGAGAAAACGTGATAATAAAGTTTGCTGATTTCTATCTAAATCAACAGTTAAAAGAGGTACCAATTTAATTTCACTATCTACTATTGAAAAATTGTTTTTTTTCAACAGCTCCTTTGCATGAAAATAACTATTTGAATGCAAGCTAATTTCAAAACACTCTTCATCATAATCAACATCAATTAAGTTAATATTGTCCAAAATTAATAATTCAATTAGATCATCTTTCAAATAGTTATTTTTATTAACAAAGATAATCCCTTCTTCTTGAAAATTAATCTTGACGCTATTTGGCTTGGCTAATTGTCCTTTTAGTTTAGCTAAATAACCGTTTAAACTACTGAGTAAACGGTTAGGATTATCAGTTAGTCCAAACACAATAATTCCAACACCATTTGGTCCAAAAATCTCATAACAAAACTCGCTAATTTTAGTTGTATCTTTTTCACTACCATGGATATTTCTTTTAATTGAATCCATGCTTAGACCCTTAGCTAAAGCAAGATCAACTGCAACTTTTAGATGTGGATTTGACTGGATATTAGTTCCACCTTTTTTAACAGCTGAAGCTATTCTTTTTGCTAGTTTTTGAAGTTGTTTTGCACTTGTTTGTTGTTTTTTATTAGTTTGATTAGCAATTAGATGCTTACGTGGCATGTAAGATTAAAAATTATTAGCAACTTGGTCAACTTTGTGTGCAAACAGATCAACTACCTCTTGCTTGTTTTTATCTTTGTAAAGAATTTTAGTGTCATCAATTAAAACACTCTCAAAATGTGTAACACCTGCAAAACCAAACAAGCCCTTTAAATTACTAACATGATCAGCTCAAGGGTATCATCCTAATGGTGCACCTTGTGACGCTAAAACAACTACCTTAAGATTAGTAAGTAATCCCTTAGAAGCACCTTTAGTAACATATTTGTCTTGAAAGGTTTTATTAGCTACAAAGACATGATCAATAAAGTTTTTTAAACTAGCAGGATAATTAAAGTTGGTCATTGGTGCAAGGATAACAATTCCATAAGCTGTTTTTAGAGCATCAATATAATAATCACTATTTTCAAAACTAAAAAAATTACTAGCGTTTTGGGTATTATAAGATATCTTACCTACTGGTAATTCATTTAAATTTCAATCAATAAATTCAACACTACTATTTTGTTTTTTATAAGTTTGAAGGAAACGATCTAATAAAAGGTGGGTGTAAGAACCAGAAGGGGTTACAGAAGCATCAACAATAATAACTTTCTTCATAGCTAATAATTAAAAGTCTAATTTTTATTGTTAATTCTAATGAGCTTTAGTTAATTTAGCAATTAATTGGTTGGTTTCAGTAAGTTTTTTTTGATATTCTTCCAATTTTAAAAATTCACTTTTTACCTTTTCTTTGGGTGCTTTTTCTAGAAAGCTTTTATTTTTAACAATAGCTTGGCTACGTTTTACTTCACTTTCAAAAAAAGCTTGTTGCTTCTGTAGAGATTTAAGTCTTTGGGTTGTATCATCAACAATTTGGTATTTAAAACTAACTTTTTTATTAGTTTCAATTTTCAGTTCAATTCAACTAAAGTTAAAGTATTGTTTAACATCAACAGCATTTTTACCAGATAAGATAACAACTAGTTTTTGTTGTGAATTAAGCATGTACTGTTTTCTGTAATTGCGTAAGTCATTAATAGCAGCTAAAACAAGATCAAAAAGTTTGGGAATTTTAATTTTAGTTGCAAGAGGTCATGTTGCTTGCATAACACTTTTATTGTTAAATTGCTGATAAATACGCTCAGATAAAAAAGGAACAGTGATACTAAGCAAAATAGCAATATTAGATAAAACTGATTTAGCAGTATAAAAAAGCTGTGGTTTTAGTTGATTTGGTTCTTTTTTAATTGCTTCAATGAAAGTATTGCAAAAATCATCCCAAACAAATTTAACAAGAATTTGGTTTGCTAATGCTAACTGGAATTTATCTAGTAGCTTAGTTATTTTTTGAATTACTTTATCTAATTTAGCTAAGATTCAAGTTTCACTTAATGAAAGTTTGTCCAAGTCATAACTAATTTCTTGATCATTTTCTAGTTGGATAACAAACTTAGTAACATTCCACAATTTATTTAAAAAATTTCATGCACTTTTTATTTTTTGTTCGCTGAAAATTAGATCATCTCCTGGAGTGTGATTTGAACACAAAAATAAGCGCACTGCATCCGCTCCATAATTTCTAATAAGATCAACAGGATCAATGCCATTATTCAGTGATTTTGACATCTTACGATTTTGTTCATCGCGTACTAAACCGTGGATTAAAACAGTTTTAAATGGCAGTTTTTTAGTTTCAAAAAAGGAGTTAAATAACATTCTTAAAACTCAGAAAAATAGAATATCATAACCTGTAACTAAAAGCTCAGTTTCATGAAAAGAGTCATCCTGTTCTCAATTCAAACAAATTAAAGGTCAAAGCGAAGAAGAAAATCAAGTATCAAGTACATCTTTTGATCTAGTGTAGTTTTGTAAATTTTTTGAAGGTTTTTCACCAACAACTATTTCACCTGTATTGTTTTCAAATCAAACAGGAATTTTATGACCCCAAATTAACTGTCTTGAAATACACCATGGTTTGAGTTTATTCAACCAATTTGACACTTGCTTATTAAAGCGTTTGGGAATAAAATCAGGATATTTTTTTAAATATAAGTGATCTTTTAACTTTGGTAAATCAACAAATCACTGTTTTGAAAGCATGGGTTCTACAACAGTGCCACTGCGTTCAGAAAAACCAACATTACTAGTTAATGGTATTGATTTAACAAGTAATTTATTTTTTTCTAATCATTTAACAATTTTATTTCTTGCTTGTAAAACACTAAGGCCTTGAAATTTACTTGCATTTTGATTGAGAATACCGTTACTGTCAATGCAGCTTAGAAAATCAAATTTATATTTAGTGTTGATTTCATAGTCATTAAAGTCGTGTGCAGGAGTACATTTCAATATTCCTGTACCAAATTTAATGTCAACATAGCTATCTGTTACAACAGGAATTTGTTTTCCTGTTAAAGGGTTAACTACTAATTTATTTCAGAAATTAGTATAGCGCTTATCTTTTGGGTTTACCAATAGACAAACATCAGCAAAGATAGTTTCTGGTCTTGTTGTTGCAACTATTAGTTCTTGTTTACTATCATTCGCTAGTTTATAAACAACATAATGAAGATGTTGATTAACAGGTTTATTGATAACTTCAATATTTGATATAGCAGTATTCAATTTTGTATCTCAATTAACAAGCGTGTATGCTTGATAAATAAAACCGTTTTCATAAAGGTTTTTAAAACAATTGTTAACAATTTTATTAGCTTGTTCTGAAAGCGTGAATTTAGTTTCAGATCAATTTAAGCAAACTCCTAAACTCTTTAGTTGATTTTTAATTATTTCGCTTTGATTTAATGCCCAATTCATGATCATTTCAGATTTTTTATCATCATCTGCATCAAAATATTTTTGATTTTCTTTTAATGCTATTTTTTCATATTTCGTTTGAGTAGCAATGCCAGCATGATCAAAGCCAGGAATTCAGTTAATACTAAATCCCTGCATCTTTTTAAAACGCATGATTTGATCAGTAATACTAACCTCAAAAGCATGACCAATATGAAGAGTACCTGTTAGATTTGGAGGGGGAAGAATTGCTGTAAAAGAATTGTTTTTATCTTTAGGTTTAAAAAAACCAGCATTATTTCAAATTTCATAAAGCCCATCACTAACTAAGTTGAAATCATAGTTTTTTTGAAAACTAAATTTATCTTTCATCTTCAACGAATAATTCGAAAATTTTTTTGTGAACTAAAGCAAGATTATGATGTGTTTTTGTACTTGCAAAAACACATTTATCAAAATTAACTTTTAAAAAATTTGCAATATTCTTAAGCGAAAAATATCTTTCACTTTGATTTAGTTTGTCAAATTTATTAGCAACAATCAAAAAGTTCAATCCTGTTTGCAAAATAATTTTAACCACTTCCTGATCTTGAACAGTAACAACTCCACTATCTACTATCAAAACAACACCAACTAGATTACTACGAAAATTTAAAAATTGGGTAAGTAAATTAGTAATAAAATCTTTCTTATTTTTGTTTATTTTAGCAAAGCCATAACCTGGTAAATCAACAAATCTTTTGTCTTTATATTCAAAGTAATTTAGTAATTGTGTTCGACCTGGTGTTGCTGAAGTTTTAGCCAGTTTCTTTTTAAAAAAAGCATTAATCAAACTTGATTTACCAACATTACTTCTTCCCATGAAACAAATCTCTGGGATATTATCTTGAGGACAATCTTTTAAATCACTTGCACTCTTCAAAAAATGTGCATCCATGTGTATTTTATTTTTGGTTTTTAATGGAGCGCATTATCTCTTTTACCTGGGTTTCAGATAATTTTCTACCCATTTTTGCATACATTGCTTTAATTTGATTTTCAGTAATAGGTGGATTGTCACGCATCTGTTTTTTAAAGATTTTTATAGAAATAAAATAACCAAGAATCATTCCCACCAAAAGCGAAAGAGGGATACCTAAACCTAATGCAAGTGCTAGATCATTCATAGCTATATATTAATAATTAAACATTTTAAAAGTCTTATAAAATTAAATAATCGAATTAATGGATAAACTTGTTAGTATATTAGTTCCTTGTTACAAATCAAAACCTTTTTTAAAACGTTTTTTTAATTCACTTTTAAAGCAAGATCTTAATCAAGCTAAAATTATTTTTTTCAATGACAATGTTGCTGATGAAACCTATGAAGTTTTGCAAAAATTCAAAAAAGAACACAATAACTTAGCAATTGAAGTCTATTGTGACAAACAGAATGAAGGTATTGGAAAAGTGCGTGATAAGCTAGTGAATCTAGTAACAACACCTTATTTTTATTTTATCGATCCTGATGATTGTTTTAACAACAAAAATGTCATAAAAGAGATTGTTGAATCAATTAAAAAAGAAGATTTTGATCTTGGTGTATTAAAAAGTATGGTCTATTTATGCTTCTTAAAACATGATTTCATTATTAAATTTTTGCCTTTAAAAGGTATTTTTCAAGGCAGAGTAAAATTAATTAATAATAACAATGTTAATAAATTAAATTACATCAAAAATAATGATCAATATATTTGAAATATTGTTATAAACACAGATTTTTTTAGGAAGCTAAATTTAACTTTTGAATCAAGGTTATTTGAAGATATACCAATCTGATATCCGATGTTTTTTTCATCACAAAAAATTGTTTTTATTGATGTGATAGGAACAAATTATTTTATTCGTAATGATAGTTTATCAACTACTATTAGTGCTCCACGCTACTTAAATTTAATCCAATGTTATGAAAAGCTATATGTAAATCTCAGCCAAAATGGTTCTCTTGCAAGTTTTATTGATCCAAATCATAAGATTGAAGCTAGGTTTTGAAGAAGGCAAATGTTTGTTTGATTTGCACTTTTCAGCTTTGAATACTTTAAGAAAAATTTTTCTGAATCAAAAAAAATTCTTGAAAAACTATTTGTTTTTTTGGAAAAAAATGGAGTTTATGAACGTGTTTTTCAAACAAAAAATCAAGGTATTTACTATATTTGGGTACAGCGACTAAAATATTTTAAACATGTTTTGGAATCTAAATCAGATAACTAATTAAGTTCTCTTTAAAAAATCAATGAGATTAAAGTTATTTAAAGCTTATTGTTTAATTTACTTAAATTAAGAATCTTTATCTTGTTAAATTAATACTTAACTGGTTTAATGTCTGCAATTAAATTTAATCCTAGTTCATTCAGAAAAAACTTTAAATGGTTTGAAAATAACAAAAATTGGATTAATTTTGATAATGCTGCTACTTCCATTGCACTTGATGTTGTGGCTGAAGCAAGCAAAGAATATTACCAGTATTTTTGTGTCAATCCTCATAACAAAAATCCTGAAATTAACCAAAAACTTATTGCTATTATTGAAGAAACAAGAGATTTATTAGCAAAATTTTTCAATGCTAAAAAAAATGAAATAATTTTTACAAGTTCTGCAACTGAATCGCTTAACTTATTCGCCTTTGGATTAAGCTCTTTAGTAAAAAGTAATGATGAAATCATTCTCAAAGAAGATGAACATGCTGCTAATGTTTTTCCCTGAGTAAATCTAGCAAAAGAAAATAAAGCCAAACTAAAAATAATTAAAAAAACACCAAATAAATCTTGAACTGATGCTTTTTTAAAAGCTTGTACACCATCAACAAAACTATTAGTTATAACTGCAACATCTAATCTTTTTGGAAATAGTATTGACTATGAAAAAATTTCTAAACACTTAAAAAAAATATCACCAAATAGCTTTATTGTTGTAGATGCAGTACAAGCTGTACCACACCATAAAATCGATATTACAAGTGCTAATATTGATTTTTTAACTTTTTCTACACATAAATTTTATGGACCTACTGGTCTTGGCATTGCCTTTATCAAAAGCGAATTACAATCACGACTAAAACCCTTTAAATTAGGTGGTGATATTTTTAAATCATTGGATAATAACTTTAAGATAATTTTTAAAGAAGGTCCTTCCAAATTTGAAGCTGGAACGCTAAATATTATGGCTATTTATGCTTTGAATAAACAGTTAAAATTCATGCAAAAAGAATTTAATTTCAGTGAAATGGTGTTTTACAGCAAACAATTAAAAAATTTAGCTTATCAACTGCTAAGTCAAAATCCTAATATCGTTTTAGCTAATCATGATCAAGATGTTCCTATCTTTGCTTTTAAGCATAAATATATTAATTCTGCAGATCTAGCAACTTTTTTAAACATTAAAAAAATAATTGTTAGACAAGGATCCATCTGTGTTGGTAAATTTAAAAATAAAGAGAGTTTTTTACGTGTTTCTCTACTCCATTACAACACAAAAGAGGAATTACTTTATTTAGAAAAATTATTAAAAACTAGTAAGAATTCCATTATTAATGAACTAATATATTAGCTAATGGATATTAGAGCAAGAACAAAAATTATTGATATTTATTCAAATTTAAAATACAAAAAACCTCTCAAAAGTTTTCAAAAAATTTTAACAACAAGTGATAGTGATAATTGTGAAGACTTTTTTAATATAGGTTTAAATATTGATAAAAATAAAATTACTGCAATTGGTTTTGATGGTGATGGTTGTATTATCTCTACAATTGCAACTGAATTAAGTATTAAAGCAATTGAAAATAAAACTATTAACCAAGCAAAAAAAATATTATCAGATTTAATTGCAACTTATAAAGATAAAAATTCAGCAAACCAAGTTGTTGAAGAATTAAAGCTTTTAATTGAAATGAATGTAACTGAAAAAAGGTTACAGTGTTTGCTTTTAACACCAAGCAACTTATTGCAATGGTTTAAAAATTTTTAGCATTAATAGCTAACGTTTGTCCTAGGATATCATATAAGCGTTTATCACCAACTAAAATCTGTTGATGTTCTCTTTGGTGTTGCTTTTGATTTTCACGAATTAAATCTAAAAATGCCAACTCTTGAGTGCCTTCATCTAAAGCTTGTTCAATAAGTAAAGACATAAAGATCTCTTTCTTTAAACCTAATGAATTAGCTTTATTATTTTTTCAATTACTAGAAATCTTATTTACATCATCATTTGTTATCTGAATAACATAACTAAGAGTCTGGATACTAGAGTCATTTGTTAATGGAACTGTTTGCACAGTTTTATATCCTTCTGTATTGGAACCATCTGTATTTGCAGCTTTAGATAAACCAACATAACCTTCAAATTTATGAAAATAAAGATCATCTAGTCTATCAACTACATTTAATAAAAACTTTTTTTTATTATCAAAACTTATTAAAGGTTCACCTCTAGAGTTAAAGCGACTTAAATTATTTGTATCAACATTAACTGAGCGATGTAATAACTCACCAAAAGCATTAAGTTCAGCATCAGAACTGGTTTTATTAATCAAGCTTCACAAGGTAAACTTATCCTTATATTGACTTAAAGCGCCTGCTTTATCAGTTGCTGTAATTAACTGTCCGTAATTATTAAAAAGCTGTTGTGTTAAACCAGCACTAGCTGTTGAAATACTTTGATTGGAATTAATTGTTAAACCATTAAATCCATAAAAATATTTTTTATCACTTGATCCTTGTGATTTATTAGATAAAGCTACAGAATTATTAAACGGTTCATCAGTAAATCTATAAGTGTTTTCACTTTCAAGCAATGAATCGGGTTGAATTGGTGTTTGTTTATCATTGAATCAGTTTATTTCAGAACCTTGAGTATAGTTAGGATTTTGTAAAAACCTATTAAAAGGGTTTTCATTAGCTGATGAAGTACTGGATTTTGTTTTTTGACTAACAGTAGCGCTAGGGTTTTTATCATATCCTACTGTTCATGAAACAAATGCTCTAGTGCCAATTGGAATTGCTTGTTGTAAAAGATTCTTATAGTTTTGAGCACCATTTTTAACAAGTCACATTAACGCTTGCAAAAAGCGCATGTATTTGTACTCAGCATCCTGATTAGGATCTTCCAATTCCTTTTCAAATGCTTTCTTTGCTAAATCTAATAGGGTTTGATTAACACTACTAGAAGTATGAGATTGGGCAGCTTTATTTTGCATTTGCAACATAGTTGAAGTTGAATTTACACTACCCAAATCCTTATAGTAACCAAAGCGTTCTTTACTAGTTAAATTTTGTCAAGAACGGATATAAAGTGCCTGTTTAACAAGATCATTAAAATCAGAAAATTCACCTTTTAATTCTTTATTTGAAGTATCAAAATTATTAGTGAATGAATTAGTGTTTTTAAAGTACCAATTTTTAAAGATGTTTGCAACAGTACCTGTTCTAATTCCTGAATCAGTTAAAAGTGATAATATAAGATCAAGATCCAAGTCATTTAATTGTGATGATTTTAGGGTAATAATCTCTGAATATTGACTAGAAGAATAAGTTGTAGCTTTTCATTTTTCAACTAATTCTTTGGCTTTATTTTCTACTTCAGTTGTTAAAGTCTTTAAAGTTTGATTATTTTTTTCACGAGTTGGTTCATTATCAGAAGTAATAGCTGCAGCAGTGGTTTTCAAAGTTAAAGTTTGAGCACTACTTTGATCAGAGTTGTTATTAAAAAATTTTGCTAATGAAGGATAACTACCATCATTTGCTCTTTTATATGGAAGAGGTTGACCCCAACCAATTGAACTTTCTTTAGCTTCCTTTTGAAGTTTAATGTAGTTGTTAGCACGATCAACAATTGCTTGTTCTAGTTTTTCTATATTAGCTAAATCTGTTCTAAAGTTATTAAGTTTATTAGCAGCTTTAATATAAGACTCATAACTTGATTTTATTTGTGAAAGTGAATTATTAAAATCTTGTTGTTTTTTAATAGTTTCAGATAAATTGGTATTGTTTTTTAAAAGTTTTTTAGCTAAATCACATACATCTTTTTGACCGTTATTATTAGAAGTTCCATTTTCTAACATAGTTGTTAAAGCTGTGTAAAGTTTTAGTGTTGTATTTCTATTTGCTCAACTTCTAAATTCGCTGTTAGAACCAAATAAATCAACTGAAAAACTGACATTATCTTTAGAGAAATTATTAGTTTGTCCTTGTAAAAATCGCATTAATAAAACTTCTGCTTGTTTTTCAAAATTACGTTTGTTAGGTGATGAATTCTCTAAAAAATAATTACCTCCATCAATATGAAATGCATATATGGCATCATCACCTCTAACATAAACAGGTTTCTGTTGCTGTTTTTGAGTTGTCTTTGAACTTAGACTAACTAATAAATTACTTGTTACTACTTGTGCTTGAGATGAATTTGATTCCATTCTAACTGCATCAAAAATAGTTGCAGTTTGACTACTATTATTTAAAGCTTGTACTTGTTTTCAAAAAGACTGCGTGTTACTACTATTATCACTACCAGAAGAAGAAAGCGAGGGATTAATTTTTAAAAATTCACTTTTAACATTAAAACCATTATTGTCTGAAGTAACTTCTTGACTTTTTGTCATAGCACTCTTCATCATAACTGTTGTTGCACTTGAACTTTTAATAAATTTATCAAGAGGATTTAAATTGCTACTAGTTCCACCTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTAATTCTGTTTTATTATTCAGTAAGTAATGGTATTGATCAATAACTGCAGCAGATAAGTTAACAGTTTTTAAAACAGAGAGAATGTTATTTTTACTAATTAAAACTTTGCTTGCACTATACTTGTTTTCAAAGTCAAGTAAATCATTTCTGGTATTAGTAGCAGCTGAACTTTGTGATGAACCACTAGAATTAGCAGTTTGTAATTGTTTATCAAACTCTTCAAATAATTTTGCAGAGTTTGTTTGACCACCACTTTCACTAGGATCTTGAAAAGCAGGAAAGTTATAAGAAAGATTTAGATTGGAAGCTCCAATTGCATTTTCATCATAAATACTGCCAAGTCCAGCAATTGGAGATTGGTAATTGAAAGAAGCTTGATTTAATAAAAATGGGTTTTCTTCTATTAATCACTGTGAAAATAAATAAGCTTGAAGATTAGTAAAAAAATCACCATTTACATCATTTAAATTGCTGTTAATAAACTTAAGTTTATTTTCTGTTTTTTCAATGTTTTCTTTAGTTGTTGCAACGCTTGTATCACTTGAATTCACTAAACTAACATTTGCACTAATGTTATTGAATATTAAAGATTGAAAATCAGTAATTAATCTATCATAGAGTTTAAGTTTTTTTCAACTTTCTTGATTACCACCGAACTGATCATATTCTTTTTGTACTTCAATAGGTCACTTTACTCTATCAGCACGTTTCTTTTCATCTATCAAGTTATCATGTTCATCTTCAACATTTTCTTCAAATATCTTTAATCGATTAGAGATGTTTCTATCTGCATTTGCTTGGTATCATTTTTGTAATGCTGATCCAAAACTAACACGTAAGATAGAATCAACACCACTACCATCATTTAAAGCAGACTTTAAAACTACATCTGGGTTTATGATATTTTGATCACTAAAACCAATTAGTTTTTGGGATCGATTGAGAGTAGGAAAAAGATTAGCAGTGTTTATTGTGGAACAGGCTGTAATTATTGCTGTTGCACCCACTCCACTAAATGCTAAAGCAATTAATGATCAGCGCTTAAAACGATGAGCCATCTTATACTAGCAGTTTGATAATTATCACATTTAAAAATCTAGCTAATATAATTGCAAATCTAGATTATTAAAAAATGTATATTAATTATAAATAAGTTAATTTTTCTAATTTAAGCATTAAAAAAGCTTAAATCTCTCTAATTAAAATGCTTTACCAGAAAATCAAAAAATTAGTTTTTAGCTAGCTGTTTGTTGAAATGAATTTTTATTAGCCAATTGTTGAACAGTTAGTTCTTTTATTTGTTCAAATAATGCTGGCTTTTCTGATAAAACATTTAATAATTTTTCTTTACCAACAGCAATGCTTTCATTGTTAAAAGAATAAGAATTTCCAGCTCTTACAACAACATTAAATTTAAGTGCTAAGTCAATAACTTCATGTTCATGTACAAAACCACGGTTAAACATGATTTCTAATATAGCAACACCAAAAGGTTTAGCAATCTTATTTTTAGATACCATTACTTTTGTTTTTATGCCAACATAATTGTTGAATTTATCCTTAAGTAATTCAACACGCTTAGCTTCCATTCTTAATGAACTATAAAATCTTAGAGCTTTTCCTCCTGTTGTAACTTCGTTATTTCCAAACATCACTCCTGGTTTTTCGCGTAACTGATTAATGAATAAAACACAAGTTTTAGAATCTGGTAATATCGATTGTATTCTTCGCAAACCTTTTGACATCATTCTTGCATGCAAGCCAATAGTTTGTTCTTCAATTGTGCCTTCTAACTCTTGTTTAGGAATTAACGCTGCTACAGAGTCAATAACAATTAAAGATATCTTGTTTGTTTTAATTAATGATTCGATAAGAGCAAAAGCGTTTTCACCATGCCTAGGATGAGCAATCAAAAGTTTATTTAGATCAATACCAATTGATTTAGCATATGCTAAATCAAGTGCACCTTCAGCATCAATATAACATGCTGTTTTACCTGCTTTCTGAAAACTAGCGACTGCATTTAGTGCAATAGTTGTTTTTCCAGATGATTCATTTCCATATAGTTCTACTATCCTACCTAAAGGTAGACCACCAGACCCTAATGCTTCATCTAAATTTAAACTTCCAGTTGAAATTGTTTCAATTTCACTGTTTTTCTTTGCATCAAAAAAATCAAAACTTGTCAAATTATTACTTTCAATAAAACTACTATTTTTTTGAGTATTTTTCTTATTAATTATTTCTTTTTGAGCCATCTTTCATTACACCTTATAAATAAGTGCAAAAAAATAAAAATGACATTTTTTTATTAGAAATTTTGTTTATTTTTTAATTTTTCTTAAAAAAATCTCTTTTTAGGAGTTTATTTTTTTATATTAGTTAACTTGTTTGGTTTGTTGACATAAACACTCCTAAAACTCCTATATATTATTAGTAAATATATAGATTTTTGTTCAAGTCTACTCAGGTGAAGCTTACATACAACAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAGTCAAGGTGAAAAACCATCCTACATCGTTGAGTTTACTAATTCCACCAATGTTGGCATCAAGTGAACGATGGTGAAAAAGTATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAATATACCTTAAACAGTAGTTTGGCCAAACAAAAGGGCAAAAGCCAGATAGAGGTACATCTTGGTTCAAATTCAAATCAGTGACGATCGATGCGCAACTCCATTGGCTTAAACAACAATCCCAGCCCCAATGCTTCAACTGGATTTAAACTCACTACCGGCAATGCATATAGAAAATTAGATCAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAATGGGTGGAGTTCAACTGAAGAAAACGAAGCTAAAAATGATGCGCCCCTAAGTACAGGAGGGGGAACATCAGACAACGCTTCAAAATTCACCAAGTACCTCAACACCAAACAAGCATTGGAAAGGATCGGTATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTACCCAACTCTATTATGCTTCTACTAGCAAGCTAGCAGTCACCAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCATGTGGTACTGGGTGGTGGAGCGGAGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGACTGAGGGGAAGACAAACAAAAACAATTTGTAGAGAACCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGCTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAAGGATTTGGTGACCAACGACACGGGATTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCTCATTCGTTGTTCCTTATTCGGGGAATCATAGTAATCAAATTTCATCAGGAACCATTAAAACTGCTTATCCAGTGAAAAAAGATGAAGCTTCCCAAGTAGCGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGAAGTGGAATCTTCTTCCTCTTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCGGTGAAGGATTCAAACAAAGACAGTGAGAAACTCAGTGAAACAACTGCTTCATCCATGAGTGGTATGGCTACATCTCCTCGCAAAGCCCTCAAAGTGGAGGTGAAAAAACAAAGTGGATCAAGTGACACCCTCACCAAAAACGACTTTGCTAAAAAGCCACTGCAGCATAAGAACAGTAGTGGGACAGAGGTGAAGTTAGATGCGAGTGGGGAGTTTGGTGACAACAAAGCCTGAAAACCATTGTTGACTACCGAGCAAATAGCAAGAGAGAAGAGGATGGGGATGCCAACTCCCCCTCCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTACTACTACTACTACTACTACTTCTACTTCCTCAACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACATTTAAATAAAGAAAACACCCGGTGGGTGTTTACACCTGGTAGTACACCAGACATCTGAACGGGAGCAGGGTATCGCGTTCAAAGTGCTAATCAGAAAAACGGCATTCCTTTTGAACAGGTGAAACCTAGCAATAATAGTACCCCCTTTGATCCCAATTCAGATGATAATAAAGTCACTTCAGGTAGCTCCTCCAAACCAACCACCTATCCTGCTTTACCCAACAGTATCAGTCCCACCAGTGACTGAAGCAATGCGTTGACTTTCACTAACAAGAATAATCCGCAGCGCAATCAACTGTTGCTCAGAAGCTTACTAGGAACTATCCCGGTCTTGATCAATAAGAGTGGAACGGGAGATGAGTTTAACCATACGAGTGAGCAGAAGTGGGATAAAACTGAGACCAAGGAAGGCAACCTCCCAGGGTTTGGGGAAGTGAATGGGTGAAAAATAGTAACTATTTTTTTAAATTAATACTCTTCAGCAACTGTTTTGCTACCTAAGAAAGTTAACTCTTCATCAGTTAAAAATCCTGTTCCTGCTGGAATTTTTCCACCAATAATAACATTCTCTTTTAAACCTAAAAGATAGTCTACTTGGTTCTTAACAGCAGCATCAGTTAGGATTTTTTTGGTATCTTGGAAGGATGCAGCACTTAAAAAGGAGTTAGATTTACTTGCCGCTTCATCAATTCCAAACACCTGATTAATTGCAATAACAGGCATCTTTCCAGCTAAAAGTAAGCTCTTATTTAGTTCATTGAGATAATGGCTATGCACTAACTGACCAACAAATAAATTGCTGTTACCTGCATCTGTTACTTGCAACAAATTAGTTAGTTGTCTGATAATAATTTCTACATACTTATCAGCAATATCTATCCCTTGAATCCTATACACTTTTTGGATCTCTACAATCATGTATTGTCTTACCCTTTGGATACCTGCAATTCGCAAAAGTTGTTTAATATCAACAGAACCTTCTGTAATCTTAGAACCTGGTGAAACTTGATCACCAACATGAACACGTATTTGAGCACTGAAAGGGATAGTATAAATCCTCTCATCAACGTTTGATTTAATCACTACTTCCTGAGCGTTTTGAACAGTAGTAATTGATTTAACTGTTCCTTTCACTTCAGAGATAACTGCTTTTTCAAAATCTTTAGGAGTAACTACTTCAAAGATCTGTTTTAAACGTTCAAAGCCTTGTGCTAAGTTGTTTTCAGTTGAAACTCCCCCAGTATGGAAAGTACGCATTGTCAATTGGGTCCCAGGTTCACCAATTGATTGAGCAGCAATCACCCCAACAGCAGTTCCCAATTCCACCAACTTACCAGTTGACAAGTCAATGCCAAAGCAGTATTGACAGATACCATTTTCCCTTTCACAATAGATAACAGATCTAACTAAAACTTCTTTAATAGATGTTGCACAAATCTGTTTAGCTAATTGCGTTGTAATAAGACTGTTAGCTTCTACAATAGTTTTTTGTGTTTCAGGATCAACTATAGGAGTAATTGAGTAGCGATTAACAATCCTGTCAAATAATGATTCAATCAAGGATTTGGTTTTGGTTTCAACAATTGCTTCAACAACAATTCCTTTTCTTGTTCCACAATCATCATGGTTAATAATTAATTCATGAGTAGCATCTACTAGCTTTCTTGTCATATAACCAGACTTTGCTGTTTTCATTGCAGTATCTGTCATCCCTTTTCTCGCTCCATAAGAAGAGTTGAAGTATTCATTAATGGTCAAACCTTCAAAAAAGGAGTGTTTAATAGGAACTTCTATCGTATCTTTAATGATCTTAGATTGGTTATTTCTTTCATAGTTAAAGCTTTTAGACATTAAGCCTCGCATCCCAAATAACTGGGTAAAGTTAGAAATGTTACATCTAGCACCTGAATCAGCCATTACCACAATGGAATTATCACGGTACTGTTCTTGTTTAATTAAGTTTTGGATCTCATCAGATACTTTTTCTTTCACATTGTTTCATAACTTCACAACGCGTTTATAACGTTCATCATCGGTTAACAAACCCTTGTTATAAAACTGTTTGTATTTCAGCACCTGTTGATCAGCACTATCAAAGTAATTTTGTTTATTGGTGTACTTAGGGATATCAAATACTGAAACAGTAGTTGAAGAAAAAGTAGAGTACTTAAAGCCAAGTGCTTTGATATTATCAAGTGTTTTAGGAAGGTCTTTAAATTCCAATAAAAGATAGAGTAGATCGATTAACTTGGAAATAACCTTTTTGGAAAATGCAGGGATAATTTGATGCTTTAAAATTGATTGTCTTACATCTTCTCCCTGTTTCACAATGAACTTTTCGGGACATGCATTTTCATCAAATTCCCCGTCATTAATATAAGGAACATTATTGCCTAAAACATCATTAAAGATAATCTTACCAACTGTTGTTATTAAAGTTCCTTGGCATGCAAACTTCTTGTTGGGAAATGCTTTAGTACTTATCCCTACAATAGCATGTAAATGAACCTTTTGACTTTCATAAGCAGCACGTGCTTCATAAACTGTGCTGAAGATAATCCCCTCTCCCAACTGTCCTTTTCTCTCTGTGGTTAGATAATAGTTACCTAAAACCATGTCCTGAGTAGGAGTTACAATAGGTCTTCCATCTTTCAAACCTAAGATATGTTTTGATGCAAGCAGAACAGCTCTTGCTTCATTAACTGCATTCTCACTTAAAGGAACATGAACTGCCATCTGATCACCATCAAAATCAGCATTAAATGCAGTAGTTACTAATGGGTGTAATCTAATTGCTTTACCATCAACAATTCTTGGTTCAAATGCTTGGATCCCTAACCTATGTAGGGTTGGAGCACGATTTAGTAAAACGGGACGATCTTTGATAACATCATAAACTATTCCCCAGATAAGATCATCCTGGTTTTTAATCATATCTTCAGCTTGTCTGATGGAAGCGGCAATTGGTCTAATCTCATTACCATTTTCATCAAACTTATTGATCAATCCATGGATAATAAAGGGTTTAAACAGCTTTAAGATCATTAGTGCTGGGATCCCAACTTCATACATCTTCAATTCAGGGCCAACCACAATTACACTTCTACCTGAATAATCAACTCTTTTACCAAGTAAGTTGTGTCTAAATAAACCTTGTTTTCCTTTTAAACGATCTGTTAAAGATTTCAATGACCGTTTGTCTTTGGAAAGTGATGGTTTATGACGTGAAGAGTTATCAAATAAAGCATCAACAGACTCTTGTAAAAGTCTTTTTTCATTGTTAACAACAATAGAAGGTACAGTACCATCTTCCAAAATCCTTCTTAATCGGTCATTTCTAATGATTACCCTGCGGTAAAAATTGTTGATGTCACTGGTGGTAAACTTAGCACCATCAAGTTGGATAATAGGTCTGATATCAGGGGGGATAACTGGAACAGTATGTAAGATCATGTTTTTAGGATGAAGCTTAGAATTTCTAAACCAACTAATAGTTTCCAGTTGTCTTAAGATCTTCTTAACTTTAGCATCTTCAACACTATCTTTCTTGGCTTTTCTTAAAGCATCATTTAACCTGCTAAATTCCAAGTTAAGATCGATTTTATTAAGCAATTCCAAAATTGCTTCAGCCCCTATTCCAACCCTAAAACCAGTGTACTTCTTAATGTAATTAAAAGCATCATTTAGAGAAAAGGGGAGAGAGCTGTTTTTTAAACTTTCATAAAAGATTTTTCCCTTACGGTAATCTTCACTACTCTTACTTTTAATGAGATTTCTGAAGATATAACCTATCACACGACGCATTTTTTGTCGTGTTGAAAGTGAACCCTTACCAGTTAAGTCCAAAACTTCTTTGAACTTAAAAGGCATGATTTTATCATCTTTGATCTTACCTGTATCAAGTACTATGTAGTTAACAAAGTACAAAACCTGTTCAACCTCTTTGTAAGAGATGTTTAAAACTAATGATATTTTGGAAGGAGATGGTAATTCTTTTGACATCCAAATGTGAGCTACAGGACTCACAAGTGCAATATGTCCCATCCTTTCTCTACGTACAATAGATTCAGTAACTCACACCCCACAGCGATCACACCTCACACCACGGTATTTAATCTTTTTGAACTTGCCACAAGCACATTCATAGTCCTTAACAGGTCCAAAGATTGCTTCATCAAACAAGCCTCCAGGTTCTGGTTTTAATGATTTATAGTTAATGGTTTCAGCTTTTGTAACTTCCCCTTCAGATCAGTTCAAAATGGTGTCATTGGAAGCGATGGAAAGTTTAATTGCTTTAATGTTTTTATAAAGCTTGTTATTTCTTTTATTACGTCTTGTTGTTGTCATTGTTAATTTCTAATAACCCTCAGTGTCAAATTCAAAATCATTGAAAAATTCATCTTGTTCCCCATCACTTTGCAAGATGGAAACATTATTGGAGTCTTGTTGGGTGTTGTCATCATAGATAAATGAAACAGATAAAGCCAAGCCCTGTAATTCTTTTGTCAATAATTTAAATGATTCAGGGATACCAGGCTCTGGGAAAGCTGCACCTTTAACAATAGCAGCATAAGCCCTATTTCTTCCTTGTACATCATCAGATTTAATGGTTAAAAGTTCTTGCAAGTTATAAGCAGCTCCATAAGCTTCTAATGCTCACACTTCCATCTCACCAAACCGCTGTCCACCATTTTGCGATTTACCACCTAATGGTTGTTGAGTGATCTTAGAATAAGGGCCAACAGCACGAGCATGGATCTTATCATCAACCATGTGATTCAGCTTCATCATGTACATAATTCCAAGTGAAATAGGTCTTTCAAATGGCATTCCACTCCTACCATCAATGAGTTTAAACTTACCCTGATTTTTTTGGGGATCTAATCCTGCTTCTTGCATAACATCTTGGAGATCTAAGAAGTTCACTCCTTGGAAAATAGGGGTTGCAATTTTGTAAATAAGATCATCAAAAGACATCCCTATCTCTTTCAAAACTAAACTAATGTCACTGTTATCGAGTTTTTCAAGTGCTTCTTTTTCACTTTTAATATTGCGATCATTGATTTGGTTTTTTAAACCTTTAATTAATCTTTCAACCCTTGCTTGAGGTTGATTGATTTCAATGGCAAACTCCTTAGCTTTATTTTGATCAAAACAACTACTAATTAAAGAACGAACTGCTAGCTTGTGTGCTGCATAACCCAAGTGGGTTTCAAAAATTTGTCCTATGTTCATCCGACTAGGAACACCAAGGGGGTTGAGCAGAATATCAACTGGGGTTCCATCTTCTAAATGGGGCATATCTTCAATAGGCACCACTTTAGAAATAACTCCTTTATTTCCGTGTCTACCAGCTAATTTATCACCAATTTGAATCTTACGTTTTTGAACCACATAAACCTTGATCATTTCAATCACACCATCATTAAGTTCATCACCATTAGCAATTGAAAAACGTTTTACAGCTGAAACAATACCATCCCCACCATGGGAAACTTTAAGTGAAGAGTCTCTCACGTTTTGAACACTTTCAGGGAAGATGGCTTTAAATAGCTTTTCTTCAGGAGAGACTTCGACTTGACCTTTAGGGGAAACTTTACCAACCAAAACATCCCCTTCTTTAACTTCAGCACCCACCATAATGATGCCATTCTCATCAAGATAGCGTTTGTTTGCATCACTAACATTAGGGATATCACGGGTAATTTGTTCATCACCATTTTTAGTAGACAAACATTGAGCAACATACTCATTAATGGTTAATGAAGTGAGAATATCTTCCTTAACTAATCGTTCTGAAATGACAATTGCATCTTCATAGTTATAACCATTTCAAGTTGTAAAAGCAACTAAAACATTCTGTCCTAATGCCAACTCACTCTTATTAACAGCAGGGCCATCAACAATGATTTCATCCTTATTAACCCTTTGGCCTATTTCAACAATTGGTTTGTGGTTATAACAAGTATTTTGGTTGGAACGTTCAAATTTAACTAGGTTAACTGTCTCTTTTTTAGAACTATCACTTGTAATAATAATCTTACTGTTATCAACATAACTAACAACACCTGAGCAAGGAGAGGACATTGTTAAACCTGAATCACTAGCAATTTTGTGTTCTTGACCAGTACCTACTGCAGGAGCATATGGCTTTATTAAAGGATAGGCCTGACGTTGCATGTTGGTTCCCATTAATGCTCTAGCTGAATCATCATTTTCCAAAAAGGGGATTAAAGAAGAACCAATGGAAACAACTTGGTGTGGTGCTACATCAATGTAATCAATCTTTAAAGGATCATAAAGTCCTTGCATAGATCGATACCTACCAATAATTTCCTTATCTAAGATCTTGTTATCGTTACTAATATTGACAAGTGAAGAGATCTCAGCAATAATATGTTCATCTTCTCTAAGCGCAGTTAAATATTCCACCTCATCAGTAATTACCCCAGCTTTGATTTTGCGATAAGGTGCCATTAAAAATCCGTTTTCATCAATCTTAGCAAAGCTAGCTAAAGACATGATCAACCCTATGTTCATCCCTTCAGGTGTTTCAATAGGGCAAATTCTACCGTACTGAGAATAATGCACATCACGGATATCTAAATTAGGGTCCTCTCTTGATATTCCCCCAGGTCCCATTGCTGAAATCCTTCTTTTATTACTCAATTCTGATAAAGGGTTTTGGTGGTCTAAAAATTGGGTTAATTGGTGGGTATTGAAGAAGTCTTTAATCACAATTTGAATTGGTTTTGAGTTGATTAAAGATTTAATAGTTAATTCCTTTTTTTCACCTTGTTCAATAACCTGACCCTCTTCATTAATTTGCTGGCCACGGTTAACTCCATCAGCAATAGTTAACTTTTCTTTTAAAAAGCGCTCCATTCTAGTGAAGCCGCTTTCTAATTTAGCAGTAATTAATTCATTAATTAACTTAACCCGCTTATTACCAAGGTGATCAATATCATCATATTTACCAATTCCATAAGGTAAATTAATCACATAACTAATTGAAGCGATGAAATCACTGAGAGTCATTGCTTTGTTCAGATCATTTTCGTTATGGATCCCTATAACTGGTACTGAAACACTAAGATTATCATTAGTGATATATACATCTATCGTTTCATAAAAGAGTGATTCTTTTGCTAAATCAACAGCCTTACCATCAGTTGTAAGTTGCATTTTATTAACAAAACTAATTTCATTGTTCTTAGCAGCTTGTTTGATTTTGTCAATCTCTTCTTTTAAAAGTAAAGTACCTTTTTTTAAAAGTAGCTTGCCATCTTTTAAGAACAAATCACATGCCAGAGTTTTTTGATAAATACGTTCTGTTAAACGTAACTTTCTAGATACTTTGTATCTCCCTGCATTAGAGAGGTTGTACCTCTTGTTTCTAAAAAAACGCGCACATAAAAGTGCATGGAAACTCACTTCATGACACTCTTCAGTGTTTTCAATATCACGCAGTGAAATAGAAAGTTCATTGACAATGTGTTTAGCAGCTCTTTCAGTAATAACACTATCCATTTTGTTTTCCATTTCCAGTTCAAGTGCAGTTAAACTAGAATCATTTGGACTTGTTTGTTTTAGCGCATTATATTGTTTCTCAAGCGTTACATATTCATTAACAAGTGATCTTAATTTCTGATCAGAAGCGATCCCTTTTCTTCTTAAATCAGTTTTACCATCTCTAAATTCTTTTAAAAGGTTTTTGATTTCAGGATCAACATTGCTTAAAAAATCCTTAGCATTGTAAATTTCCGCTTCCAATGAACGTTTGATAAATTCATTGTTTTTAAAGATTTTAAGGATCTCACGATTATTTAAACCAAACGCTTTTAAAAGTGTTGTAATAGGGAAAATCTTAGCACCTTCTCTTACCGCATCTCTTAAAAGAATTTGTACAAAAGCATCTTCAATCTTTTTTTTATTGGAGATGTATATAAGCATCACTGAACCATTAGCAGGTAAAACCTCACAAACATAACCTTCCTGTACTCTTTTACGGGAGTTGGATAGTTTTAGTTGGGATTTTGTTAGCATGTATATCCCTGGAGAACGGGTTATTTGGGAAATAACAAACTTTTCTATCCCATTGATAATAAAAACCCCATCATGGGTTATTAAAGGCATGCTAGCTAAAAATACACCATTTGTATTGCTAGCAATATTTTTCTTTGATTTCCGTGCTTTTTTAACTGTTCCTTTATCATTATCAACTAATTCAAGATCAGCATAAATACCAACTTCATAAGTTTTAGATTGAGCACGTGATTGTGCTTCATCACGTTCTGGTTCAGTTCTGTGTAATCCCCTAAAATTGATAGTGTAGCGATCATTAGGGGATTTGATTGGAAAATATGAGGCAATTAATTTTTCAAGATCATGATCTAAGAATTTTTGGTAGCTTTTAATCTGAATATCAGCTAAATTTGGTTGGATAAAATTGGTCTCAATTTTGCCATAATACCTTCTGGTAGCTGTAGGGGAATAACGTTTTTGAAAGAAATTAGATTTTTGTGACATTTGTGGGGTGAAAAGCGGATCAAAACATTAAAAAGCCACTAACAGCGTTATAAAACGCATGCTAATGGACTTAATCACAGTTCGCTAAATTATATTAATTTTTTAACTTGTTCAATAAAAGGCTTAAAATCAAAGTTCTCATGATAACCGTTGACTAAAACTGTTCCTTTAACATTAAGACCGCTAAAACTTAAAAATTTATCTAGTAATTCTAGAACAGTTTTACCCCCACCCGCAGTTGCTGGAGTTACACAACAAATAACTCCATTAAGACCTTTTAAAAATTGATTTCTACCATACTGCTCATCACGAGTCATCCAGTCAATGATGTTTTTAGCAAAAGCAGGGATAAAACCGTTGTGTTCAGGGGTAACAAAAATTAAATTGCTATGTTCTTTGATTTTTCTAACTAAAGTTTTAATTTTGTCAGGAAAGTTAGTTGCTTCTAAATCAACAGAATAAAAATCAACTTGATAGTCTTTTAACTCAATCAATTCACAACTTAAAGAATGTTTTAATTCATTAGCAAATTTCCTGTTAATGGAATGTTGCGAATTGGAGAGCATTAAAATTAATGACTTTTGATCTTTTGACATAAATTAACACTTAAATTTTAAATAAAAAGTATTTAAAAACAATTTAATAGAAGCGTATAGCTATTTTTTTCAATGTTGTTAATTATTGAGAAAATAGCAACCTTGATAAGTCTTAATATCAGCAACCTTAGTATCTAAACTAACGGTTGGTAAATTGGGGTTTTGTTCTTTATTTCCTTCAAGTAAACAGATACAATTCTCATCAAAATTAAAACCTTTCATTATTTCAACAATTAAGTGGAATTCCAAATCAAAAATCAAAGCATCAACTTTTAATTTAGTAGGAGAATTTGAAAATACTTGTAATTTGGAATAATGCAGTAAAAACTGCTGATTAAAATCTTTAACAGTTTGTTGTCAAATTAAGTTCTTTTGCTCAAAGTTAGGATGCTTAATGTTTTCTAAATTTTTTAACAAGTATTTATGAGGCAAGATAAAATGGCTGGTGATTTTTAACTTTTTTTCAATTGTGAATCTGTCTTGAAAACTAGTGAAACTAAAATCAATGTTACTTTTTTCAAAGTTAGTTTCATAACCAAACACACCGTTTAGTTCAACAAACATTACGTTATTTTTAGACTTGTGCTTTTTTTCTAATTTTCTAACTAACTTAAAAAACTTATTTTGAAATGATCTTAAAGAATAAACTTCATGTTTTATTTTTTGTTTTTTAATTTCTTTAATTAGCGAATCAGAAAGCTTAATAGTTGTTTTTTCTTCTGGTGAATTAATTAATAAAAAAACTATTTTCAAACCCTTAAAAGTTTTCAGTATTTTTAAAAACTTATTAATTTCTGTATCTGTATTTTTGGTTTTTTCAACAAAAGCAAATACAGTATCGTTTTGACTTTTATTTCTTAATGCTAAACCAAATGCACTTCACAAGTTATTATCAAAACCTAAAAAAGGGAAGTTATCAAAATAAATCTTTTGATATATTGGAAGACTTACTTTGTTAACAAGCAGATAATTTACTTTTTTAGATCTAACTAAAAAATTAACAAAATCTAGATATTGTAATGTTGATAAAAGTCCAACCTTATTGCTATTTTGAAAAAAATCATAAGCTAATAGGCGTTGCTCATTAACGAGGGTTTGATCATTAATTTTATGAGACATTGTTTAATAAATTTTACTTAGTTTATTATGATTCAGGATATCATCCATTGCTTTACAAAAGCTTTCATATTTACTATTAAACATTTGGTGTTCACTGTTTAAGATAACAGATGATTGCAAATTATTGTTCTTAATTAGGTATTTATTGAAATAGCTTAATGTTAATTTTGTTGGCATAAATAAATCATTTTGAGCTAAAACCAAAAACACTGGTAACTTTAAATTTTTATAAGTCTTATTTAAAACTGGTAGTTCTTTCAAAATATTATTAGAAAGCTTTTTAGCCATTTTACTGTGTTTTGACAGCTCATTTTCTCAGCTGTTTTCATCAGTTAAATTACTAAATTTAGTTTGAAACATTGCTTTAAAATTAGCCATTCTTTCTGCCTTATCTTTTTTAAAAGAACTTTTAATTTTGAAATATCTCAATAAGTTACAGTATGAAAGAGGTGCTAAAAGTATGAGTTTTTCAACACGTTTAGCATTTAATAAATTCATGGCATAACTACAAACAGCAGCACCCATACTGTGTCCTATTAGAACGATGTTTTCCAGATCCTTATTTTCTATAAAAGCAGCTAATAGTTCTCCATAATGGATTGGAGATAATTCCTTATAATCAAAACCATTTACATTATGACCAGGTCAAAGTGGAGCGTAATAGTCATAATCAGTAAGGGTTTTATAAAAACTTTGAAAGTAGCTATGAAAAACAGAAAATCCATGTGCAAAAACAACCGTTTGTTTTTTTGTTTTTTTTGCTGGCTGAAAGAAAACTTCAAGCTTAGAAATATCAATCTGATTAAGATCTGAAAACCTTTTCACAATTATTTTTTTAACACACTTTAAAACAACGTGAGCACAAATTATGATCTAATTTTTGCTTAATGATTGTTTGAAAATTTCAGCATCTTTCACACATTGTTTTTTTAGTTTTTTTAACTTTAATTTCATTTTCATTATTTGTTAAATTAACACTGTTTACATTAAGTCAAAGTGCGAGATTATCCTTTAATTTTTTGGGTATTTTTTTATTAATTCCAACGGTTAATTCAATTTGATTATTTTTAGAAATCAACCCTTCTTTTCTTAGCTTTTCAATTTCTTTGAAAGCAGCATTTTTAATACTAGTAAAAGTTTTATAGATATTTTCTAAATTCTTAGAGTTAGCAACTTTAAAAACAGTCGGTTTTGTAAAGAGGTTCACACTTATTGGTTTTTTATTGAATGAATAGTTTTTTCAAGCATCTTCTGCAGTGTGGGGAATAAAGATATTTAAAAAACTGATTAGTTGTGTAAAAATATAGTTTAAAACAGCTTGTTTAGCTAAACGATTAGGATTATTTTTAGCATCACAATATAAGGTGTCTTTAATTATTTCAAAGTATCAGCTTGATAGTCATAAAACAAACTTATTAATCACTTTTAGGCAACCTAAAAAATTATATTTCTCTAAAAATTTCTCAATTTGTTCTACTAGTGAATTAGTTTTATGGATAACTATTTTGTCTTCTAGTGAAAACTTATAATCATCCATTGATGTAAAGTTAAATCCATTAATATTACCCAAAATAAAACGTAGTAAACTATTTCTAATTCTGCGGTATTGTTCAGCAACTTGTTTAAGAATATTAACACCTATTTTGTTATCAATTTGTCAATCAGTATTAGCAACTCACAACCTTAATATATCCGCTCCATATTGATCACAAATTTTTAAAGGATCAACTATGTTTCCTAATGACTTTGACATTTTATTGCCATTTTCATCAAGTGTAAAACCATGTGAAACAAGTGATTTAAAAGGGATTAAATCATTTTGAATAATTCCGCAATTTGAAGAAGAGTTGAACCAACCCCGATATTGATCAGAACCTTCAATATAAAGATCAGCTATTGAACCATATTTATTTATTTCCAAAACATTATAGGAAGAACCTGAGTCAAATCAAACATCTAATGTATCAATCTCCTTGTGATACTTAACTCATTTTTTGGTTTTATCAGGTTTTAAAAAACAAGTTACATCTTTTTCAAATCAACTATCAATACCATGCTTTTTCAATTGTTTAATTGTGTATTGAATTGTTGAAAAATCTAACAATGGTTTGTTATTTGCATAAACAATTGGTATAGGCAAGCCCCATACTCTTTGACGTGAGATACATCATTCATCACGTTGTAAAAGCATCTCTTTTAATCTCAATTGATTTTTTGAATTTAAAAAATTAACTTGATTAATTTGTTTTTTTAACTGCTTTTTTATTGATTTAGTTTTAATGAATAATTGTTTGGAAGCACGGTATATAACTGGAGTTTTTGAGCGTCAATCATGTGGTTCGCGGTGGGAAATAACTTCAGAAAAAATAAAGCTATTGTTTTGTTTTAAACGATTAATAATTAGATCATTTGCTTTAAGATAAAAACAATTCTCAAGTTCTTTATCATTAAGTAAGTTATTAAATACACCTTTCTCATCAATAGAAATCAAAACTTCTTTAATCTTGTTTTTTTGACAAAGATAAAAATCATCAATTCCAAATGCAGGGGAGCTGTGAACAATACCTGTTCCCTCATTATCAACAACATGTATTCCCATTAGAACTGGTAAAACCTTGTTATAAAAACAATGAGAATAGCTTGAATTTTTTAAATTTGAACCCTTGAATTTTTTTAGTTTAATTGCATTTGTTCAATTTAACTTATTTGTAAAAACTTCAAATAATTTTTCCAAGATAACAAATTTTTGTTGGTTATATTCAAAAAGAAGATAATCAAAATCAGGATGAATGGCAATTGCTTGATTAGTTGGTAGTGTTCAAGGAGTTGTTGTTCAAACTAATAAATTAGCATTTTCATCTAAAAAATCACTTTTAGAAACTTTAAAAGTTAAATAAAGTGCAATTGAATTAACTTCTTTATATTCAATTTCCGCTTCAGCAAGTGAAGTTCTTGAAATTGGTGATCAATAAGTTGGTTTTAAATCTTGAAAAATGAGCCCTTTTTTAATTGCTTGTAAAAATAGTTCAAGTTCCTTAAATTGAAAACTCTCATCTATTGTGTAATAACAGTTTTGAAAATCATTTAAAAGTCCCAGTCTTTGAAATTGTTCTTTTTGAACTGCAATTTGTGAAAGTGCAAACTGATGACATAATTTTCTTTTTTCAACAGTTGAAAGATTGCTATAACTACTAGGGTTTTTCTTACTAACTGCATGTTCTATTGGTAGTCCATGACAATCCCAACCAGGAATAAAAACAACATCATATCCTTCATATAACCAACTACGTAAAATGAAGTCTTTTAAAATCTTGTTAAGAGCATGTCCCACATGAATACTACCATTTGCATAAGGTGGTCCATCATGCAGTATTTTTATCTGTTTTCCTTTATTCTGTTTTTTTAATTTTTGAAAGACTTTTTTATCTTTTCAAAAATCATGAAAATTCTTTTCACTAGTAGATAAATTTGCCTGCATCGCAAAGGATGTTTTAGGCATTAACAATGTCTTTTTTAAGTCCATTAAATCAGAGTAATTTTATTAATATTTATCTAACTTACATCTGATGTATAAATCAGTAATAAACATAGTTTTATTTTGTCCAGAAATTCCTAATAACACTGGCAACATCGTACGTAGTTGCACTGCTTTTAAAGCTAATCTACACTTAATTAAACCTTATGGCTTTTTCTTAAATGATAAAAGGATGGTTAGAGCTGGTTTAAATTGTTGAGATAAAATTCAATTATTTGAACACAAATCATGAGAACATTTCTTACAAGCAACCACTGAAAATAAAACTATTTGGCTTTTAACTAAAAGTGGTGATAAAACTCCTGATCAAATTTGCATGACAAATAAATTACCAAACGAACTTTACTTTGTTTTTGGTCAGGAAACAAAGGGATTACCTAAAACAATCATGGATAACTTTAAACAAAACCAAATTAGAATTCCCATTTGAAATAGTGTTAGAAGTATTAATCTTGCTAATGCAGTTGTCTGTATTTTGTATGAATATTCAAAGCAAAATCAATACTCTAATTTAGATAAACAGTGCGCTTAAGAAAAGTTAAAAACGCTCTTTTAAAAATTAATCAAAGTCCTTATTTTTATTCAAAAGATAAGTTTGCTAAGTTTACTAAAAAACAATTAGTGCTGGAATTGGGTTGTGGTAAGGGTACTTTTTTAATCAAAGAAGCACAAAAAAATAACAATTTTCTTTTTATAGGAATTGAACGTGAACCTACAATTGTTTTAAAAGCAATTAACAAAATTAACAAGTTGGATTTTAATTTGGAAAATATCTTATTGTTGTGTACAGATGCAAAACAACTTGATGATTATTTTCAAGCTGAATCTGTTCAAAAAATCTTTATTAATTTCCCTGATCCTTGACCTAAAAAGCGTCATATACAAAGACGTCTAACAAGTCCAGATTTTTTGAAACTTTTTTGAAATTTACTAGTAAAAAATGGCTTAATTGAGTTTAAGACTGATAATGATAAGTTATTTGAATATACTTTAACAACATTGCAAGAAAATAGTCAAATTTTTGAAATTATCCATCAAATAACTGATCTTAACAATTCTGAATTCAGTTTTCAAAATAGTATCACTGAATATGAACAGCGCTTTATGGAATTAGAAATTCCAATTAAAAAACTAGTGATTAAGAAAATAATTTAATTAAAGTTATTAGAATAATTATTAGCTTGATGGCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCATTAAAGATTCAAAGAGCAGCAGTTAGCTGCTTTTTTTTATTAGCTAGTTTAGTGGAGTATAAACACTATCCAAGAAATTTAAAAAATCAACACTAACTCTTACTGCGTGTTGTTTAATTCCATAAGATTTTTGTGATCTTTGAACTGCTGGTTGAGAAGATTGTGGTGAAGTAGGAGTAGTATTTTGTCCATTACCACTACTTGAGTCACCACCTGAACTGCCATCACTACCGCCCCCTTCACCACTTGAAGCTGGTGGGGTTGAATCACCCTGACCTGATCCAGGTGCATTAGGATTTGTAGAGTTATCCAAACCATAAAAATTAGCTCTTATCTTATTAAGGTTATCTTTTCTAAAAGCAATCACAATTGCTTTAGTTACATCATTACCAACTGTTTGAGCATGCACATTTGATTGATTGATTAAGTTAGCTACATTACCACCATAAGTACGTTGAATAAAATCAACTATTTCTCTATAGCGAACTGCAGAAGCATCTGATCTTCTTAACTTATTGGAATTGTCTTTATCTTCTTGACTTGCAAGTTCGTTTTTTCTATCCACAACAGGAAAACGTGCATATGGGTTTCACTTTACTGAACCAGGAGCAATATCATTCCAAATTAAAAATTTAACTCCGTTTGGATAAGTAGAATAACGGTTTTGCACCTGTCTGATGGTGGTACCCAAGGTACCTCCTCAACTAAGAGAACTTGTACCTTGATTAGTAGAACCGTTTAGAAAATTAATTTGGGAACTATCTGTTTCGGTTGCAATAACAACCACATAATTACCATCGTTATATGATCTGGTTCCAAAAACTAAGTTTCTAAATGTTGTACTGTTAACAGGCAATTGGTTGTAAGCTGTATTTTTTTCCACACTTTCAACAGAAAGTTCACGAAAAAAAGACTGATCGATGTTACTACAAGAACTTAAAATCATTCCTGTTCCTAGTAATAAAACTAACCATGCTTTTTTATGTTTTAGATTTCAAAAAAGACGCTTCATTTCTAAATTGACTGGTGTCGGAGACCAGACTTGAACTGGCACAGTCTTTAACGACCACAAGCACCTCAAGCTTGCGTGTCTACCATTCCACCACTCCGACTATTTGCTTGTAGTTAATATTGTTTATATTAATAAAAATTTTAATACAAGAGTTAACAAGGCTATTAGCTTGTACTAGCTTAAACTAGGAATATAAAGGTGTTATTTCAAGCTAAATTCCCGCTTTTTTCTCCTTAAATTCAACTTTTTTTATCCGATTATTGGTAAATAAATCAGTTTAAAAAGTTATTAACAAAATGTTTATAAGTCTCAAAAAAGGGTTGTTTTAGACTGTTTTTAATGTCAATAAGTTGTAAATAAATGGCAGAAAAATCTTTTTTTAGCCTTTTAATGCAAAGTAATTTCAATAAATGGAGGATAGCGTCTTATACTCTTTTTACTTTTGAACGTATATGCAACCCAACTATTACCGTGTGATCAAAAAAGCAACATCATTTTCTGGATTAGAAATGATCTCTCATGCTTATGGTCAAATTGCGGGAGTGGAAGTTGTTGGGTTTTATTTATGACTAATAACAGAAGCTAATATTCAGGCGTTCAACAGTGAAATTAGAACCCCCATTTCACGATTACAAAATGCCTTTAATCCTTTTGAAATTAAAAAAAACCAAGTCTCTGAATGGATTTATAAAACTATTAGCAAATTAGAGAGTCTTGGTTTAGTGAGAACTTTTTTTTCACCAAAACGCTCTGAAATAACTTTTTGTATTATTGATCCTTTAGATTGGAAAGAATTTAAGCAAAACAAACAATTAAAAGAAAAACTAGTTGAGGCAATGGGAAAAGTTGAATATGACCGAAACTGCTTAGCTTTTGATCAAATCGACAATCTTCAATTTGATAATGCGCTTGAAATCTCTGCTAACTTTGAAGTTAATTTCACTGCAAACCAAAGCGATGTTTGATTTAGCTTTAACTTCGAGGAACTACATAAAGAACTTGTAAAAAACAAACTTTTAATTTCTTTAGATGAAAAAGCTAAGACTTTAATTAATGGTTATTTTGAAAAATACAAGCTTTCATTGCAACAAATTACTGATTGCATCATCAACAGTAGTACTCAAGAGAATGAGCTTGATTTTCAAAAGTTAGAAATGATGTTTTTTCAAATAGTAAAAAATGATACAGCTCCCATTTTAGAAACAGTTTCAAACAACAAAGACTTTTTTTATAAGAATGAAATTTTAGATGAATCAACAAAAAAAGCAATAACAGACTGTCATGTAAACTTCAATTCTGAAAAATACCTTTTTCTTCTATATGGAAAGATAGATGAATCGCAATTGCAATTAGTTAGACAATTGAGAAGTGATTATCAACTATTAGATAAAGTAATTAATTTAGTATTAGACTTTTCCTTTTGAAAAAATAACGGAATGTGAAGAGAAAAATACATTCTAAAAATTGCCCAATCCATCAAAATTAATAACAGTCAAAACAGTTATGAAAAAACCCTTAATAATTTTATTAGAGCACTAACTTTAAATAAAAAGCATTCTTTAAATAATATAAAACCAGTTGAAAAAACAATTTCCTTTACTGAGTATTTTGAATTTATTAAATAATGAAAATTTCACCAGATCAAAAAGATAAAAAGTCATTATTTAAAAAACATTTTGCTTATTTGAAATTAACTGAAAAAGAACTTAATGATCCTAAGATACAAACACTTTTAGAAGTTGCGTGGAATCATTTTGATCAGTGTAGAAAAATTAAAGAAAGAAGATGTAGTAATAAAGGTGATATCCATCTTCAAGCTGTTAGAGAGTGGTTACCTTGAGAATTTAACGCTAATCAACTTAAAAAAAATAGTACAAAAGAAACAACGCAAGTTAGTGAAAATGAATTTATGGGTAATGTTATGCTCATGCAAACTATTTGTCCTAAATTAGTTAATAAAGCAAACTGATTTGATTTAACTTATGAGCGTTTTATTGTTACAAAACCAAACTGATTTAAATACATGGATTTGATTCCAATGATCCAAAATTTACCAGTAACTCCAAGTGATAAAAATAGTTTTGGTTATGCATACAAAAAGATATCTAATCTTTTTGAAACTGAAAAGAAAACAAAGAAAAGGATATTTTTTTTCAATTTACAAAAGAATAACATTAACAATATGGCAGCTTGTATGTTAACTTGTGAAATAGCTAAGAAAAATATAAAAGTTGCTTTAATTTATTGTGAAGAATTTGTTAGCAGATATGACAAAAGTTATTGAAAGGTAGATGATGATTTAAATTTACTTGATGAGGCAAAAGTAATCATATTCATTGGTTTAGGTCAGGAAAGTTTTCATAACAAAAACTATATCTTGTTTATGACAAGGCTTTTTATAAATTGTTTTTTGAAAAGAAAAGATGTTTTCTTTTTTAGCACTACTTATAGTGATGGTAATGGATTAATTCAAACATTTAAAAACCAAATTATTAGTAGTGCAAATTGAGTAAAACACTTTTTTGAACAACTTAATGATTTATTAATGATTAATATTTAAAAATTACATTCTTTCACCAATAAATAGCTAAGTTTATGTAAAATTTATCTTGTATTACCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCATAATTAAATACTTAAATTTACACTACGAAATTACAAGCTCAGTTTAAAGCTGAGCTTGTTTTATTTTTATAGTGATTTCTATTTAGCTAATGCTCTTTTTCAATAAAACTTTTGAAAGGAACGCATAATTGGCACTCCAAAACAACTAACAAGTCCAAATTTCACCAAATTAAAAAACGAATATAAACAGAAAATTCCCAGCCAATAGTTATTAATTCCAAAGAAAAAGAAATGAAAATCAGTGTTCTTGTTATATTGTTCAGCAACTATTAAAAAGTTAGGTGTTTGAACATAACCAAAGTAGTATCAAAACAAAGGTGTTATCAAAATACCGTTAAGCGTTGTTAGTAATAAAGCATTGGATAAGGTTGTAAATAAAAACACAAAGATTCAACAAAATCTTCTTTTTGAAAATAAACAAAAATAAAGCCTTGTAAATAAGATAGTCGATAAGTTAGCTAAAGTTACAGTTAGAACACCAATAACATTATTAGTATTTCAAAAAAAACTAGTTAAAGCAGAAGCTAATGTAATTGTTAAGCTTCAACCTAGTGACACAAAAAAAGCACATGCAACTAAAAAAACAACGGAAATATCAAAAGTTAGTTTTAGTTGTGAGAAAAAAGGAATACTAATAAATTCACTAATTAATGATGTAATGATGGCCAAAGCAAGCATCATTGCACTAAAAGTAATATTAAATAACTGATGGTATGATTTTTTTGGCAAAAAAACCAGTTTATTACCATTCATTATTTAAAAGACTCTTGAATTATTAGTTAATAATAATATTTATTGATATGGACAAATTTTTAATTGATGTTATTGTAGAAATCCCTAAAAACAGCAAAATAAAGTATGAGTATGATCGTCAAACTGGTCAAATTCGCGTTGATAGAATCCTATTTGGAAGTGAATCATATCCACAAAACTACGGTTTTATTAAAAATACATTAGATTGAGATGGGGATGAACTTGATTGTTTTATCTTTGCAGATCAACCATTTTTGCCTGCAACAGTTGTGCCTACAAGAATTGTAGGAGCACTTGAGATGATTGATGATGGGGAAATTGATACTAAGTTATTAGGAGTTATTGATTGTGACCCTAGATATAAAGAAATTAATCAAATTAGTGATTTACCTAAACATAGAATAGAAGAAATTCTTATCTTTTTAAAAACTTATAAATTACTTCAAAAAAAGACTGTAATTATTAAGGGTTTAAAAGATGTTTGTTGAGCTAAAAAAGAATATGAAATTTGTTTGCAATTAATGAAAGATTATGGTCATTTATCAAAAGATCAATTTATCCAAAAAATGCAAATTCTTCATCCAGAACATTACCAAAAGTAAATATGATAAATGCCAATCGCGGAATGCTTTTAGAGACAATTGTTAATCAAACAATTGCTAGGTTAAAGGATCATCCAGATATTTGACTGGAAAAGCGGTTCTTACCTATTAAGCCTATAGCTTTTCGTCATGCTCATGTATCTGGCAACGTATCACAAAAATCAAAAACTGATTATTATGGAATTTACAAGGGAATGTATTTTGATTTTGAAGCAAAACAAACAAATAAAAGTAATTTTCCAATTGCTCAAATAGCAGAACACCAATTAAATCATTTGAAAAGAATTGACCAAATTGGAGGAGTTAGCTTCTTGCTAATTTACTTTCAAACTAAAGATCAAATTTTTGCATTTCACACTAAGGATCTTCTAGAAACAATAAAAAATCAAGAAAGCAAAACAATTAAAAGGGAATTGATAGAACAAAAATCTCAGAAAGTTCCACTATTGTATCCTGGTATTATTGATTTAATTTCAATAATTCAAAGCTTTAAAAATTATTAGTCTGCGTACTTTCAACGCACTTTAGCTGCAGTTTCTTTGATCGTTTTGCTTGGACTGTAGCGCACTAATGGTTTTGGTGGGATATGTCTAATCTCACCTGTTTGCATATCCTTCTGATAACGTGCATTTCTAATAGTAATCCTTAATTTACCTAAATTTTCAGGTAATATACAAACACTTCTACTTACCAATTCATTTAGTAACAATGTGTTAAGGTATTTAAAGATTTCCTTAATTTTTTTTTCTTTTATACCAGTAGCAACTGCAATTATTTTATTGATTTCACTACGAGAAAGTGGCTTACTTGTATTTGATGTTTTTTCCATATAATTATTTTTTAAAACTTTATTAGCTCTGTTATTGCTAAAAACAAAAGAGAAACAATTTAACGCATTCTATTTTATTATTGATTCAACAATTAAATTAAAACACTTATAAAGTAAATTTTTAGACAATTATTTCTATTCTTAAGACATGGAACAAAACAATATTAAAGAACAACTTATTTCTTTCTTTAATCAAGCATGTTCTACCCACCAAGAAAGACTTGATTTTATCTGTTCTACAAGAGAAAGTGATACTTTTTCTAGTGTTGATGTACCACTTGAACCCATTAAAAATATTATTGAAATAACTAAAGATGAAAATCAACAAATTGAAATTACAAAAATAGCTGTTAATAACATTAAAACATTATCTTCTGTTGGTGCAACCGGTCAGTATATGGCATCTTTCTTTTCAACGAATAGTGAGCCAGCTATAATATTTTGCGTCATTTATTTTTTATATCACTTTGGTTTTTTAAAAGATAACAATAAAAAACAAATAATAAAAAAGGCTTATGAAACTATTGCTGATAACATTGCTGACTATTTAAATGAAAATTAGGTTTAAGACTTATTATTTTGTGTAATGGTAATATTGTCTTTAACAACTGCAATGTTGTATCCAACACCTTTTTTTATCTTTCCCTGAAGGATATATTTAGCTATTAAAGTTGCAACACTATGATCAATAAAGCGCTTAATTGGTCTTGCACCAAACTGTTGATCAAAACTACTTTTATAGATAAACTCTGTTAGATTTGAATCAAAATTAAAAAATAAATTTTGTTTATTCAAGCGTTTTGAAAGTTGTGCCAACAAGCTGTTGATTATCGATAAAACTGTATCTTTCTCAAGAACATTGAAAAATACTATCTCATCAATACGATTTATAAATTCAGGACGGAAATGTTTCTTTAGACTTTGAATGGCCAAATCTTTTTTTCCTTCTAAAAGAAAATTTGAACCTAGGTTAGAAGTCATAATTATCAAAGTATTTTTGAAATTAACAACCCTTCCTTGTGAATCTTTTAAAGTACCATCATCTAAAACTTGTAATAAAACATTAGTTACATCAGGATGTGCCTTTTCAATTTCATCAAATAACAAGACGCTATAAGGTTTTCTTCTAACCGCTTCAGTTAGCAAACCTGATTGTTCATAACCTATGTACCCTGGGGGTGCACCAATTAATTTAGCTACTGAATGTTTTTCCATATATTCACTCATATCAAAACGAATCAGAGCTTTTTCATTGTCAAAAAGAACTTCTGCTAATGATTTGGCAAGTTCAGTTTTACCAACACCAGTAGAACCTAAAAAGATGAAAGAACCAATAGGTTTGTTTGGGTCATTTATATTTACTCTACCTCTAATTACAGTGTTAACAACAGCATCGATGGCTTCATCTTGTCCTTTAACTCTTTTTTTGATTTCATCACCTAAGTGCAAAAGTTTATCCTTTTCACTTTCTAATAGTTTTTTAAGTGGAATTCCTGTTGTTTGTGAAATAACTTCAGCAATTTCATTTTCAGAAACTTCAGTTTTAAATAAATCGTGCTTAGAAGTTGCATATTTTTGTTGTGCACTTTCCAGTTCTTTTTTAAGTCTTGGGATATCAGAGTACAGAATTTTAGATGCACTTTCATAATTTCCTTCACTTTGGTATGTCTCTAGTTTGGTTTGAAATTCTTCAATCTCTTTTTTGAGCTTATTAATGTTTTCAAAATCAGCCTTTTCCTTTTTTCATTCATTTATAAGTGAATCACGCTTTTGTTTAAGAGCATCTAATTGTTTTTTTAGTTTCTCTAAATATTCTTTCTTTGATTGTTTGTTATCATTTTCCTTATCTTGTTTAAGAGCTGCATACTCTGTCTCAAGATTGATTATTTCACGTTTAAGACTATCTATTGCAACTGGTTCAGATGACATTTCTGTTTTGATCTTAGCAGCAGCCTCATCAATAAGATCAATGGCTTTATCAGGTAAATTACGTTCATTAATATAACGAGTTGACATTTCAACAGCAGCTACTAAAGCACTATCAAAAATAGTGATGTTATGAAAGAGTTCTCAACGTGTTTTTAAACCACGCATAATTGTTAGTGCCTCTTGACTACTAGGCTCGTTAATAAGAATTTTTTGAAATCTACGTTCTAAAGCGCCATCTTTTTCAATGTATTCCCGGTATTCTTTTAGAGTAGTAGCGCCAATTACTTTTATTTCTCCTCGAGCTAGCATCGGCTTTAATATATTGGCAATATCCATTGCACCACTGCTAGAATTACGTCCTAATCCAACTATTTGGTGAATTTCATCAATAAAAAGAATAATCCTGCCATTTGATTCTTTTACTTGCTTAAGAATGGTATTAATTCTTTTTTCAAATTCACCTTGGAATTTAGTGCCAGCAATTAATCCAGAAAGAGATAGTTCATAAATTTCTACATCCCTTAAATTTAAAGGTACATCATTACTAACAACTCTTCTAACAAAACCTTCTACTATTGCGGTTTTACCAACTCCAGGTTCACCAATTAAAACAGGATTGTTTTTGCTTTTACGACTTAATATCTCAATTAAACGACGAATTTCGTTATCTCTTCCAATAATAGGATCGACCTTATTTTTTAATACTTCATCGTTAATATTACGACCAATTTCTTGCAAAAAATTACGATTTTCACCAGCAGGTGTGAAATTAATATTCATTTTAATTGCTCACTCCTTTTAATGCAAAAATTATATAAAATTAGCACTCAAGGCATTCGAGTGCTAATTAAAAACTAGGTTTTTTGTTTATCTCTAATTTATCTAATCAGAGTTGATATTTTTGTCAAAATAATTAGAAACCTAAAATAACTATTAACTATTTTTTTGTGCTTGTTTTCAAATTGAGGGAAATAATGCTTTTAAAAAGTATTCTAATCGTTTTTCAACTCATTTTCGATAGTTTAAAATTGCTTCTGTTTGGGGCATAATCTCAGTTCATTGAAAAGCAAATAATAAAACATAAATAACTGTTTCAAAAACAAGCTTACGCTTAAGTCCACCATAAATATCAATAAGTTTATTTGCTGTTTCTATGGTTAGTTCTTCACGAATAATATTAGCTATTTCATAATAAGGATTATCAACACAAGATCATTCAAAATCAATAAGGATTACTTGCTTTTTAGGTGTTCAAACCAAATTATCAAAAGTAGCATCATGATGACAGAGAGTTTTTGGTATGTCAATATGCTTCTTAGTTAAATTAAGATATAACCGTTTATAAAAAGCGGAAAGCTTAGTTTTTTCAAAATAAACCAAAGGATCAAATATTAAAAGTTCATTAGCAAATGGTTTTCAGTCAACTGAATGAACTTTTTTTAAAGATTCAACCAATCTGCATAAAAATAGATCAGTTGCTCTTTTAGGTTGAGTGCCTTCTATCCACCTTTTTATAGCATTACCTGTTGTTACATCAAAATAAACAAATGGGTTTGAAAAAAAAGGTTCTACTAGTTGCAAAATAATTTGTTCATTTCTTCTGTCTAAAATAACATCTTTTCTTGAAAGTCTTATTTGAAATTTACTATGATCTTTCAATTCACAAAAAAAAGAAAAATTAGTAAAACCAGAATGAATTTGCTCTATCTTGAAAATATCTGTTTCTACAACTTGAAGTTTTTCACAAAGAAAAGCAACCGCTTTTTTTAAATCCATTAATTTTGATGATTATTTTTTAATGTTTGTACTTACTCTCAAAGCATCTTCTACAATCATCAATTCCTCATTTGTACGAACTCTAAAAATTTGATACTTGCTTTCATTTGTTGAAATTAGAGAACTATCTTGATAATTTCCAAATAAATTACTGTTAGTTTTAAAACCAAGTGAAGCAACTTTTTCAATTATTAATTGCACACAATAACTAGCATTTTCACCAACTCCCCCAGTAAATACCAAGCTATCAATTTCACCTGAAAGTTGATTTAGGTATTTAGCAATATAGTCAGCAACACGATTAACATACATTTTTATAGCAATATCATTAATTTCTGGTTTATCAAAAATATCACGCATGTCAGAACTACCTGTTATAGCAAACATTCCACTCTTTTTATTTAATTCATTTACAACATCATTACATGAAAGCTTTTGCTGTTCAGCAATGTAACTAACAATGGCAGGATCAATATCACCACTACGTGTTCCCATTATTAATCCTTCAAGGGGAGTGAATCCCATCGATGTGTTTAGTGATTTGCCTTGTTTAATCGCACAAACACTTGCACCATTACCAAGATGACAAACAATTAAATTAAGTGGTTTTTTATTAAACTTTTTTTCTAAAAACTCGTTAATGTATTTATAAGAAGTTCCATGAAAACCATATCTTCTTACTAAGTTATTTTTCTCTCAATTTTCAGGAACTGCATAAAGATAATTTTCCCTTGGAATAGTAGTGTGAAAAGTGGTATCAAATACAGCAACATTCTTAGCAGTTTTTATCTCTTTTAGAAAAATTTCAATAACATCTGCTTCTGGTTTATTATGAAGCGGTGCTAACTTAATGAATTCTTTTATTTTTGCTAGTGAATGTGTATCAACAAGAACTGCATCTGTAAAATAATTTGCTCCTTGTACTACTCTATGCCCTATTAGCCCAATTTCTGAAAGTTCAGTAATAATTTTGTTTTTTTTAAGCGCATTTAAAAAATGCTTAACAGCAAGATTATGATCATTAAATTGAACCTTTTCTTCTATCTTTTTTTGATTAAATTCAAGCTTAAAAAAACCATCAATGAAAATACGTTCACAAAGTCCTTTAGCTAGTACTTGTTTTTTATCATTAAAAAGTTGAAATTTAATTGAACTGCTACCTGCATTAACAACCAAGATTTTGTGACTTTGCATTTAACCGAAGAATATTTACCTTATTTATTAAAAAATAATACTAAATTAATATAGTGTGGAATAATTAAATGTTTTAACTAAAAAGAAATATTATTAAATCTTAAACAATGAAAATTAAAAAATGCTATATTATCGCTGTTATTTCAGCTTTTATTGGTTTTCTTTTTTACTTTTTATCTTCACTTATAGGGATAACAGATGTTAATAATGCAAAAACAGTAGCAACTGTATTTTTTGTATTAGCAACATTAATAGTTGTTAATGCTATTGTCTTCTGGATTCTTTATCTATTCAAAAAAAAGAACGTAGTTAAGTAAATTTATTTGTCACTAATAGTTTTTTAGCACATTAATTTGATAGAAAACAAATTGAATGTAATATGGAAACTATCAGAGAAACACTTTCTTCTATACAAAGAAGCAATTTTTTATTAAATGAAAGCGCACAAATTCTCAATACGTGTGTAAACAAGAACACATTGCTTGACGATAAGCTGCGCAATTCGATTAATTTTGCACTTAGCATCATTAAGCTAGCAGACTTTTGTCTAAAGGATGATACTGAAAAAATTAATCAATTCTTAGGTAAAACATATGATTTACCATGAGAGTTTCAATTTAACCAAAAAGACAAGCATATAGAATGCACTGTCAAAGACAATTTTGGTCGTGAGAAACTAATAAATTTGATTTTTCAAATTGGTGATGCTATTGAAACTTATCATACTACTTTAATAAGATTCAAAATTCCCAAGCATTGTTTGAATGCAAGGGATCAAATCAAAAAAATAATGGAGGGCAAATAAAAATGATTACATCTATCTTTGGAAAAGTTACTTTTGTAGGCAAAAGAAAAATAATTGTTGAGCACAACTGGATTTCATATTGATTTAATACAAAAGAAAACCATAAATTTGAAAAAAATTTGGAAAAAAATAAGCAAATTTTTTGTCATATTATTAAAAAAATTGTCGCTAACCAAATTATAGAAGAGGCTTTTGCCTTTAATACTCTAGAAGAAAAAGAGTGGTTCTGTAGATTAATAGAACTCAATGGTATTGGTAGTAAAACTGCACTTAATTTGCTCAATAATGACCTTGAGGAAATTAAACAATACATTCTGGAAAATAACTACAGTGCATTATGTGGTATTAACGGTGTAAATAACAAAATAGCTCGTGCACTTTTATCACTTGAAATATTTGAAAAATCTGAAAATAATAAAAATATTAAAGGAGTTCAAGTTGCTGATGGTTATGATGAATTGTTTGAAACACTAAAGTCACTTGGTTACAAACAACAAGAAATTCAGGATGCACTAAAAATGATAGAAGTAAAACCTGATTTTGATATAAGTCAGTTAGTTGCAGAAGTAATTAAATTAATGTCTTTTAAGAATAATGAAATTACAAATAAAACCGCCTAATACCTTTGATGAATTTGTAGGAAAACAAGAAATAATTAGTCAAATTCAATTAAGTATTAAAGCATCTAAATTAAATAAAACACAACTAGATCATATCTTGTTATATGGCCCACCTGGTGTGGGTAAAACTACTTTAGCCAGATTAATAGCAAATGAATTGAAAACAAAGTTGCAAATTATTCAAGGTGGACATTTACAAAAACCAAGCGATTTCTTAAACGCAATTTCACTCATTAAAAAAGGTGATGTTCTTTTTATAGATGAGATCCATGCCGTAGCACCTAATGTCATGGAACTAATGTATCCAGTTATGGATGTGTTCAAAATACAAGTATTAATTGGCAAGGATTTTAATTCCAAGATAGTTGAAATGAAGGTAAATCCTTTTACTCTAATTGGTGCAACTACACAACTTGGTAAAATCATCAATCCTTTAGAAGATAGATTTGGCGTTATCTTAAACATTAACTATTATTCAAATGCTGAAATTGAAAAGATGGTAAGTATCTATGGAAAGCAAATGAAGTTAGAGCTAAATTCAAATGAAATTTCAGCTATCACTGAACATAGTAAACAAACACCAAGAATTGCAATTAGAATAGTTAGAAGAATATTTGAACAAAAAATTGTTAATAAAAAAATAGACCTTGAGGGTTTGTTTAAGAATTTAATGATTTATAAAAATGGTCTGCAAAGTATTGATGTCCAATATCTTGAGGTTTTAAATCGCCAAAATGAACCACAAGGAATTAAGTCAATTAGTTCCATGTTAGGTATAGACAGACACACTATAGAAAATAAAATTGAACCTTTTTTGTTGCGTGAAAATATGATTCAAAAAACCAAAAAAGGCAGGATTATTACAAATAGCGGAAGAGAATATTTAGTTAACTTTTAAGCTTATTAGCTCTTTTAATAATTTCAAAACCAAAACTTTCATTAATATCAAAAATTAATTTATCAAGTGAAGATTCTTCGCTTAATTTGCTAATTGATTTTGGTATGAATTGATAAAATAAAGATTTTTTCTGTCCTTCATCTGTATCTATCTTTTTTAAATCAAAAAAACTAATACCTATTAATCGAACGTTCTTTTCAGTATTAATTAATAGTCTGTTGAATAATCGTTTTGTTATTGACAGTAGTTTTCTATAATCGTTTGAATATTTTTTCATTTTATTTGAATGAGAATTAACAATAAAATCATTGCTTTTCAATTGCACAACTATCCCTTTACACACTTGTGAAGAGAGTTGTAATCTTATAAAGAGCTGATCAAATATTTGCGTTAACTTTTTATTTAACTGATTGTTTGAATAATTTAAATCTTCTAACGTTTCACTGACTGCAAAAGAGCGAGACTTAACCTGATTGTTATTATCTGTATATCATTTGCCTAGTGAAACTGCTTTTAAAGATTCTCAAAAATTACCAAATACTTTTTTTAAAAGCGAAGCATCTTCACAAACTGCTAGATCATTTATTTTGTAAAAATTATTTTTAAAAACTAAATCAATATGTTTTTCTCCTATCCCTGGAATTTCAGTAATTGGTAGTGGTCAAAGTTTTTTTTTAATATCCTTAACTGAACAACTTTTAATTCCAAATGGTTTTGCTTGATTTGAAAAAATCTTAGCAATTAAAAAATGATCTGAGATGCCAATTGAAATTTTGATTCTCAAGTTTTGAAAAACAAAATTTTTTATTTTTTTCGCTATTAAAAATGCCTTTTTAAATGAAATGTTTTGAAAACAAGCAACCCCTTCATCAACAGAAAGAACATCAATTTTTAATGAAAAAGTGCTTTCAATAACTGAAAAAATACGTTTAGAATGTTTTCTGTAATTACGAAAATTAGAATGTGCAAAAATAGCATTTGGACATAGTTCTAATGCTTTTAAAATAGACATTCCAGATCTAATTCCATAACTACGAGCAACATAGTTACAAGTTGAAACTACACTTCTTGAAAAGCGATTACCAACTATTAAGGGTTGATTAACTAATTCTGGATTTTCTAATTCTTCAACAGATGCAAAAAAAGCATCAAAATCAAAATAAAGAAAAATTAAATTTTTATCAATTAAATATTCTGGTTCAAAATATGTAAAGGTGTTAATCATATTTATCAAGTTATGTTATTATTTACTTGCAATAAAACCAAAGAAAATAACGAGTTTTTAACTTTAATAAAGTTATCGAGGATTTATCATCGTTTGTGCTATTGCGAATATTGTAGTTAATGGTAGATAGTAAGAAAAATAAAAAACAGCAGGTTACGGATTTTTCTAATTTACTCTCTCAAAGTAAAGGATTTGTTATTTTTGACTATTCAGGAATGTCTGCTGTTGATGCAACTTTAATGAGAAAAAAGTTGTTTAATAAGGGTAGTAAGATAAAAATTGTTAAAAACAATATCTTAAGACGTGCTTTAAAAACTAGTAATTTTGAAGGTGTTGATGAATCGGTCATCAAAGGAAAAATTGCAGTTGCTGTTGGTATTAACGAGATCTTAGAAACCTTAAAAGTTGTTGATAGTGTAGTTAAAGAAAAAGAGTTAATGAAATTTGTTTGTGGTCATTTTGATAACCGTATTTTTAATAGTGATGACTTACAAAAAATAGCAAAACTCCCTGGTAGAAATGAACTTTATGGAATGTTTCTTTCAGTTCTACAAGCACCATTACGAAAATTTCTCTATGCTCTTCAGGCAGTAAGGAATGCTAAGTAAATTAAATAAATAGAAAAATATTATGGGAAAACTAGATAAAAAACAATTAATTGAATCTCTAAAAGAGATGACTATAGTTGAAATTGATGAAATAATCAAGGCTGTTGAAGAAGCATTTGGTGTAACTGCAACTCCAATAGTAGCTGCTGGCGCAGCTGGTGCTACACAAGAAGCTGCTAGCGAAGTTAGTGTAAAGGTAACAGGATATGCTGATAATGCTAAGTTAGCTGTTTTAAAACTTTATCGTGAAATTACTGGAGTTGGTTTAATGGAAGCTAAAACTGCAGTTGAAAAATTACCTTGCGTTGTAAAACAAGATATTAAACCAGAAGAAGCAGAAGAACTTAAAAAGCGTTTTGTTGAAGTTGGTGCAACTGTTGAAGTTAAATAAAGATGGCAGTACAACAACGGCGTTCTAGTAAACACCGTCGTGATAAAAGACGTTCTCACGATGCACTTACTCTACAAACTTTAAGTGTTTGTAAGAAATGTGGTAAGAAGAAGTTATCACATCGTGTGTGCTCTTGTGGTATGTACGGTGAACTAAGAGTTAAAAAAGCTCACTAATTCACAGATAAATATTATTGTTTTTTTGCAGTTGAATTTGTATTACTTTTATGCAAAATAACAGCATTTTTTGATTTTAAACGCTTAGCTCTGTTTAAAGAAATAATCCCTTTTCTGGCTAAACGATCTGCTTGAGAATAAACAGAACTGAGATTATCTAAATTAATCTCTTTATTAAATTTTTTAACATTAGTTTTTAGTTTAGTTTTTTGTCCTTTATTATTTAAATTTCTCTTAATGTCTTGACGTAATCGTTTTTCGTTAGATTTAATATTAGCCATTTAGGTTATTAATTAGTTTTAGAGTATATTATTATCTTGATTATATTGTAACTAGAACCATATATGGATGGTGGACAACAAGGGAGTTTTTTTGGGCTTTTAGTAATTGTTATTCCAATAATTTTGTTAATTGTTTTTTTTTCCAAGAAAAAAGGGGCACAAAAAAATGATTTTAGTGGTGAAGGAGGTAATCGATCATCAAGAAAAGATGAAGTGTGAAAAACAATTAAACAGTTTTTGCAAGAAAAGAATGAACGTGGTAAAGAAATTATTAAAACTTTTGTAGCTAAAAAACCAAACCCTTTACATTCAAAAAAAGACCGCAAGCTTTTCAATCAAGAGATACAGGCATATATTACTAGTAATAACTTAGGAAAAAGCGAAGCAAAACGTTATAAAAATGAGCAAACTCGCTTAATGCAAAGAGAACTTTATTGTATTTATTTTGTTACAAAAGATGCTAAATCAACTGAAGTTGATGATGCTAGGATTATAGAAGCTGAGGTTTATCAAAAACCTACAAAAACCAAAAGTACTCCAGAGCGGCTAATTCGTATACTTGGTTTAAAAAATTTTGAAACTGAAATGCAATGAATTCAACCATTAATGGTTCGTGAAGAAAAGAGAAAAGAAAAAGAAGAACAGAAAAAACTTAAATTAGCTGCAAGAGAACTAAAAAAGAAGAAAAAGAAGAAAATAAAAAAACCAAAAGAAATCAGAAATCAGAAAAATGTTTAAAATTGTTTTCTTTGGTACTTCAACGCTTTCAAAAAAATGTTTAGAACAACTTTTTTACGATAATGATTTTGAAATTTGTGCTGTTGTAACTCAGCCAGACAAAATTAATCATCGTAACAATAAAATAGTACCTTCTGATGTTAAGTCTTTTTGTTTGGAAAAAAACATAACTTTTTTTCAACCAAAACAAAGCATAAGCATAAAAGCTGATCTAGAAAAATTAAAAGCTGATATTGGTATTTGCGTTTCATTTGGTCAGTATCTTCATCAAGATATTATTGATCTTTTTCCAAATAAAGTAATTAACTTACATCCTTCTAAGTTACCACTACTTCGTGGTGGTGCACCATTACATTGAACCATTATTAATGGTTTTAAAAAATCTGCATTGAGTGTAATTCAATTGGTTAAAAAAATGGATGCAGGTCCGATTTGAAAACAACAAGATTTTTTAGTTAATAATGACTGAAATACTGGTGATTTATCCATATATGTAGAAGAACATTCACCCTCTTTTTTAATTGAATGTACTAAAGAAATTCTCAATAAAAAAGGGAAATGATTTGAACAAATAGGTGAACCTACTTTTGGATTAAACATAAGAAAAGAACAAGAACATCTTGATCTTAATCAGATTTACAAGAGTTTTTTAAACTGAGTAAAAGGTTTAGCTCCCAAACCTGGTGGTTGGTTAAGCTTTGAAGGAAAAAACATCAAAATTTTCAAAGCTAAATATGTTAGTAAAAGTAATTACAAACATCAATTAGGAGAGATAGTTAATATATCTCGAAAAGGAATTAATATTGCTTTAAAAAGCAATGAAATTATTTCAATTGAAAAAATTCAAATACCTGGAAAAAGGGTGATGGAAGTAAGTGAAATAATAAACGGAAAACATCCTTTTGTTGTTGGTAAATGTTTCAAATAGAATGATTACAAAGAGTTTTTTCCTTAAAAATTTTGATCGCTCTAAAGAATTAATTCCTCTTTCATATAACGATGTTTTTAGCGCTGTTAATCAGTTTCTGAAAAGCTATACTGACGTTAATGATATTGATATTATTGAAGAAAATTTGATTGAAGATCCAGTTTTTGAACTTGATGTTTTAGAACTTTTAAACGAGGATCCAATGCTATTATTGGACAGTAAAAATCCCCGTGTTCAAGAAGCTAAAATTATTGCTAATAAAGCAAAAAAAAATATTAAAGATTATTTTAATCTTCCCATTTTTTTTGATACAGATAGTTTAGATAAAAATGTTTCTGTTTATCAAAAAGCTGAGCTTACTGAAAAGAAAATTCAAGAAATAATAACATCAAAAAAATCAGCTATTATTTTTAAACCTATTTTTGAAATAGAAGATTGCTTAATTCAACCAGATGCTATTATTGTTCATGAAAAAGGACTTTGTGAATTTGTTGTTATAAAAGCTACAACCAATACAAAAAGAAAATATTTTTTGGAAATAATTTATGACTTTGTGTTATTTAAAAAAATAGGTAAGTATAAACTTTTAAACTATTATTTTTGTACTGTTAAATACGAATTACAAAATAAAAATAATGTTTCTTTCTTTTTAAATACTGAAATAAAAACATCAAAAAACAGTTTTAGTTTAAGTTCAAAAGAAAAAGATTATTTTAAGAATAAACCTTTTAATCACCCTGAAAAAATTGCTTACATACACAAAAAAAAGAGCAATGGAGTTAATGGTTTTTTGATTGTAAAACTCATTGATAACCTTATTAAAAACAATATAGTTGATTTAAACAAAATAAGTGATTTTGTTACTAAAGAAATTGACAGTAAAAGTGTTCGTAATATTCAACCACTTATTAAAAATGCGGCTAAAATACAAATTAATTTTTGAGATCAAATACAAGATATAAAAAAATATCAAGAACTCAAAATAAATCAAATTGTTTTTAATTACAGTGAAAATTTTGATTCTTTTTGGAGTAACTATTTATTAAGAAATTTAATTAAATTAGTTTTTGCTCATAAATACAATGAAATTTTTAAATTATCTGGTAAATTAGCTAATTGAAGTCAATTAACATATGCATATAAAGAAAATAAATCAATAACTATAAATCAACTGCTTCACGAATTAAATCAGAAAAAAAGCAAGGCTAATTTTAATAACTCAACTAATAAAATAAGTTTTTTTCTTGAAGCGTGAAATAGTGAAAAAGGTTTTGCAATTGGCAATAAATTTAAAAATACTTGAAATAAACTTAAAAAAAAGAAAGTTTATTTTGATTTTGAAACAATTAGTTCATCAATCAGAATCATAAATAATTCATTACCATTTAGTCAAATTGTTACCCAATGCTCATTAATAGTTGATAAAAATGAAATAGATGATAAAAGAAAACTTAATTGTGAAAATCTAATTTTTGATCCCTTATTTATTAGCGTTAATGACTTTAAAAAAGTAATTGATTCACTCTATCAAAACAATTGCAGTGATTATAGTTTTGTTGTTTTTAATAAATCTTTTGAAAAAAACAGATTATTGGAAATGGCTACATTGATTAATGAACAAATATATAAAGAAAAAGTAAAAGCTATTGTTGACAATCTTTTTGACTTAGCTGATATTTTTACTATTGAAAATAACTGTTTGGCTTTCAAACAGCTAAACGGTTTTTCCTCTATCAAAAAGGTTTTAACTATTATAGATGAAAGCTTTTTAAAGGCAAGTAAATCAATTGGTTATCAAAATTTAAAAATCCAAAAAGGTGATGTAGCACAAGAAGTTGCTTTATCGCGTTTTCTAAATTGCTTAAATAAAAATGAATGAAATCAAGTAGCTTTTGAATTAAAAAAATATTGTGAAAATGATGTCAGAGCGATGATTTCAATTGTTTTATTTATCCAAGATTTAATCAAAAAAAATGATCTATTTACTTTTTATTCAGAAAATTAGAACCTGCTTTTCTACTATTTCACCTAATTTTTTTTAGGTTTAATAGCTAGTTTCTTACACATCTTTTGATTTTCTATCTTCTCTTTTAAATCACTAAAAAAATGGCTTCCTTTACTACTTTTTAAAAAACTTGTAAAAGAAGCCATTGCGTTGTGCTCTAAAACACTTTTATCATCGTAATTTTTGTTATTTGGGATAGCTTGTGATCAAATAATATCACCATCAAGTTCAATTATACTAAGTACCAAATTATTTGAAGTTAAGCTTACCCTAACTCTTGGTTCAGGTAATTTTTGCTCTTGAAAAAGTGAAAAGAAATCGTATTTTAAAATCTCAGAAAAAGTTCTTTCAAAAATATATTTTTCAACAAATTCAGTTGCTTTTTTCATCCCCATATCTTCATAAATGGCGCCAACCAAAGCTTCAAGTACATCACCAACAGTGTTTTCAGTTAGTTCAGCACCATTGCTTAACTTAACAAAATCACCTAATTTTAGCTCCATACCAATACGATTAAGATTTTCACCCTTAACAATTTCAATCTTAGTTCTTGTTAAAAGACCTTCGTTATATTTAGGATAAAGTTCAAATAGTTTTTTAGCAACAACAAAGTCAATTAAAGCATCACCTAAAAACTCTAAGCGATCGTAACTTTCACTAACATCTTCATGTTCATTGATGTAAGAAGCGTGAATAAAAGCTTTTTCAAAAAATTCTCAATTATTAGGAAAAATATCTAAATTCTTTAAAAAAGTTGCTAGTTTTTTATCAAAAATCTTATTATTTTTTAGTTTCAAAACTTTATTCTTCATAGTTATTCAAAGAAGTTTTAATTGCATTAATAAGATCAGATTTTAAGCTTAGATGTGCTAATCTAATCGTGCTTAAAAATTGTTGTTTATCAGCAGAACCGTGAGTTTTTAAAGCTAATTTATTTAAACCCATTACAACAGCTCCAGCGTTATTTTTGTAATCAAATTTTTTGGCTACACTTTTGATAATACCTAAGCTAAATAAGCCAGCAAGAGGATTTCTTTTATAACCCCTCTTAAGAATCCGTGCTATCGTTTTAAAAGTACCTTCCATTGCTTTTAAAACTAAATTGCCGCTATATCCATCAGCTATCAAAATATCACATATTCCATCCAGTAAAAACCTTGATTCCACAAATCCTAAAAAGTTAAGATTTTTATCAGCTTTTAAAAGCTTAAATGCTTCTTGATGATAATCAAAACCTTTATTTTCTTCTGTTCCTATATTCAATAGTCCAATTTTAGGAGTTTTTTTTGTAGTTGTTTTTTTAACAAAAATATCAGCCATTAAACCTAAAAAATAAAGTTCTTTTCCTGTGAAATATTTATTAGCTCCAACATCCAAAAAATAAAACCAATTATTGTTATCTGTCGGTACATAAGACATAAAAGCACTCTTAGTATTTTTATTAATTTTGCCAAAAGCATCATTTGTTAAAGAAGCATAAACTGCTGAAGAGCCTGCTGAAATTACAACATCAGCATTACCTTCACGAACTAAGTTTATGGCTATTTGCATTGAACTGTTAACTTTTCTTCTTGCACTTAGTGGAGTGTCGGTCATTTCAATGAAAGAATTAGCAAGTTTTTTTGTAATATTTTTTGGAAGTATATCAAGACCATCAAAAGCCTTTTCATCACCGATCAGAATGAAATTTAAGTCCTGATGAAAACTCCAATATTTCAATACTGCTTCAATTGCTTCACTAGGTTTGTTTTCAAAACCTAAACAATCAACTGCAAATCTAAAAGCCATTACTGTATGCCTATTATGTAAGAGAACACTTTTTGTTCTCCAGGTGAAAATTCACAAAACAATTTATATTTTTTTTCAACTAAAGCTTGCATTTTTTTCGCATCTTGTTCAGTGATGTCTTTGCCATAATAAGCCAAAAGGAACTCAGGTTTTTTCACTTTTTTTGAAAGGATATCAATTGTTTTAAAAAAACAGTCTGTAAGTTGAGATTCACTAGCAATAATACTTTTATTTGTAACAGCAATAAAATCATTTTTATTCACCATTACTTTGTTTTCTTTATATGACTTTGAAGCTTGTGTTATCGTAGCGGAAGCAAACTCTTTAATAAAGCGCTTCATTGCTTTCACATTAGTGTGAATAGAAAGATCACTGTTAAATACAGTTAAAGCAGCAATTGACTCCACAGGATTTGCTGTAATAACATAATCTGCATTAATCTTTTGGTGTTTTAACTGTTTAATTGTTTCATTGGCAGATAGAAAATAGTTTTTATCATGTAACAAAAAGATTACATTACTAGAATTGGTTAACTTAACAGCCTCAAGTAAAGAAAAAACAGAAGGAGCCCCAGTATCATCAGTACATAATATGGCGTTAATATCATGATCTTCACGAATTCTTTCAGCAAATGCTTCTGTAGGTACAGTAGCTACTATAGCAGGTTTTAAAACTCTTTTTGTTGGTGAGTTATTCTTGTTATTAACCTGTAAATTCATGTTATCAATTTTGACAAATTCAAATTCACCATAATTAAGACCCATTTCTAATAAAAGATGTGGTTTTAAAGTATGAGCATGAACTTTAACAAAACCATTGTCTTTATCACTAGCAATTACTACAGAATTGGCAATTCTATTTACTTTACTTTCAAACTTTTTTTGATGAAATTTTTGCTTTTCAACTGTCTGATTTATTTTTAAACCTAGTCTTAAAACATATTCTGTACAATAACCAAATTCATCTTCATTAGCATGTTGTTCTTTAAACTTGTTAAATTTAATTTCTATAGTTGATAAAGTATTGTCATCTAAATTAGATTCGCCCCCATAATATGACAACATTCCTTCAAGAAAACAAGCAAAACCATAAGCACCTGAATCAACCACACCTGATGCTTTTAATACAGGTAACATTTGGGGAGTTTTTTTCACTGCTTGTCAAGCTACTTTAACAGCTTGTTCAAAAAGTTTTTCAAGTGTTTTAGGACGATTTTTTTGACTCTTAAACTCATTGCTTATTAAACGAGCAACTGTTAGCATAGTTCCTTCTACTGGTTTTGATACGTTTTTATAAGCCACTTCTTGAGCAACAATGAATGCATTTGCAACATCTTCTATACTCAACTCTGAATTATTGCTTGTTTTATTTACGATTATGTTACTGAAAAATCCTTTCATTATTTGTGAAAAGATAACCCCTGAATTGCCACGTGAAAACAAAAGTAAATCTCTAGTAAAGTTTTTACCAAGTTCACTAAAGCTTTTTATATGACTTTTTTCATTTTCTAGTTTCTTGATGGCTTCTGTTATTGTTACTTTCATGTTTGTTCCAGTGTCACCATCAGGAACAGGAAAAACATTCAACTGGTTTATGTATTCATAATTTTTAGCGATGTTATTACAGCCAAGGCGTAACATATCGATAAAAGAAGATAAATTTACTGATGACATCTAAAGTAAAACCCTAGCTTATAAGGGATAAAAAAGAAGTTTATAACCACAATAATTTTAAATTTTAACTATTGAATTAAATATTCAAACCAAGGTACTATTTTACTCTTGAATTGCATTAATGAATAATTTGTTAAAATGGGAAACTTGCTCGTTTCTAGTTTAGGAAAAACTAATTCATAAGCGTGAAGTGCTTGATGCTTATATTTATTATTACGATTTTTAAATTGAATCAAGCTGTATTTTTTATCACCAACTAGTTGATTTGAAAATAAATTTAAACACGCACGAATTTGGTGAGTCCTACCACTAATTAATCTAATTGTTATTAAAGACATATCTTTTTGAGCTATGTATGAAGAATTTTCAAAAAAAGTGCTTATCTTTTTTGCTTCTGGAAAAGGTTTTCATTTTACTGTTACAATGCCATTATTGTTATCTTTTTTTCAATAAGCAGTTTGTAAGCCAAGATGGTTAAATTGACCAAAAACTAAGCCTTTATATCTTTTGGTTAAATGGTTATTTTTAAAAACTTTGTTCAATTCTTTTAAAGCTTTGTTTGTTTTAGCACCAATAACAATTCCAGAAGTATTACGATCAATACGATGTGCAAACTGGGGATAAAAGTTAAGTTTATTACTGTCAAATTGTCTATATCCACAGTGTTTTAAAAGCATATTTGATAAATTAATAATGCTATGTTTTTTATCAGGTTGGCAAACTATTCCAGTTGGTTTGTCAACTACAATAATATTTTCATCCTCAAATATAATTTTTAGCTTATCTTTTACCAAATCTAGAGATAAATAGTCATTATTAGTTTGTAAATAGGGATTTATATCAAAAAGAAAAGCTATCTCATCTTTAGTTTTTAATCTTGTGTTGACCTGTGGTTTTGTTTTATTTAACAAAACCTTTCCTAAGCGTAAATACTTAAAAAAGTCACTTCTTTTAATTAAGGGAAGGATTTTTAAACAAAATTGGTCAATTCGTTGGTTTTCACTTTCTTTAGGAACTAAAAATTTATTAGCTATTCTCATTTTGTTTGGCAAGAAAATCATCCATTATCTGTACTAATTTCATAAATTGATTTTTTTCATCCAAAACAAAAGCAGCTGCATATTTATGTCCACCACCATTGAACATTTGTGCAAAGTTATTAATTGGAATATTACGTGAGCGAATGGAACCAATTCATTTTTTAATGGACTCGTTAAAGTAAACAGTAGTCCATATTTTTACTCCTTTTATGTTATTCAAAGCATGAACCATTGGTAAAGGAGAGACAACACCAAAATGTTTATATGCTCCTTTTTTAATCAATGCATAAGCTAACCCATTTTTAGTTATTTTGGCTTTGCTAAGTACATAGCTAAAGTACTTATGTTCTAGTAATGGCTTTAAATAAACTGCATCATGAACCTTATTGCGGTTAAAACCAGTTTCCATCAATTTTGCTGTTAGGGCAAATGTTTGGGGTGTAGTAGTTGGACCTCAGAATCGCTGTGTATCAGTAATTATTCCTGCATAAAGATATGATGCAATCTCATCATTTAATTTGTAACCCATCTGCAAAATTAAATAACCAATCATCTCAGCAGTTGCTGAAAAAGAAGAATCAATTCATTCCATATCAGCAAATTTTTCAGTTCTAGGATGGTGATCTATCCTAACTGTCTCTTTTGCCAACTTATGTTTTTGAGTTAAAACTCTTTCTTGGTTGGAAGTATCAAAAATAATAGCTAAAGATTCTTTTACAAAATCATCATTTATGTCAGTTTGCTCAAAAGGAAAAAGTTCTCTACCGTCTGCATTAATATTGTATGAACCCATAACATAAGCTTTTTTTTCACTGAAAAAAGTATTTAGGAAAGTTTTGAAAGCAAAAGCAGATCCAAATGCATCAAAGTCTGGGTTTACATGTACAAATAAACTGAACTTATCAAACTGTTTAACCTTTTTTGAAAAATTCTTAATGAATTGTGGATCGATACTAATCATTGCTTAATTCACTTACTCCCTTTTCAATTAAATCATTTAAAAAAATAAGCTCACTTTCAAGCTTTTCAACATTTATCAATTTAGCTTTCGTTGTAGGATTTTTGGGAGAAAACTCACTACAAGTATCTAAATGTTGTTCAATAGAAATATCAAAAGTATTAAAAAATTTAGCTAATTCAATAATTTTATCTTTACTAAAGCCAATTAAGGGGCGAACTATAAATGTATCTGGTGTTGCTGATTGAATAACTTTTAAATTTTCAATGGTTTGGGATGCAACTTGTCCCAAAACCTCACCTGTAACTAGACAATCATACTTAAACATACTAGCAGCTTTATAAAAAACACGACGCATTAAAACGATACGATAAGATTCATTACTAATATGAATTAACTCTTTTTGAATTGCAGTGAAATCAAAAACTAATAATTTACCACTACAAATCGTTTTATTAAAAGAAATTAAATTTGCTAACCTTGTGATTTTTTCAATTGTTTTTTGATTCTTATTTGGTTCATTAATAAAGGTGATAAAATCAATGTTAAAACCACGTTGCATTACAAGTGAAGCTGCAACTGGGCTATCAATTCCTCCTGAAAGTAAGACTAAAGCTTTACCACTACTATAAACAGGTAATCCACCTTTACCTTTAAAACGTTCTGTAAAAACAAGAAAATGCTCCTTCAAAATCTCTATATTAGCTATTATTTCAGGGTTATTAATTACTCCTTTTAATTGGTATTTTTCAAATAGTTTAACTGCTAAATACTTCTTAAAATTACTTGAATTTTCAGCAAAATTTTTATCACGTCTCTTAACTTCTAATTTAAAGGAATTAAAATCTTTAAATAAATTAAAAAGAAGATCTAATAGTTTGTTTTCTTCCCTTACTATCTGTGATGCAAAAAAGAACAAACTAATTCCAGGCAAAAAACTGAATAATTCTTGGAGAATCGCTCTTTGTTCTTTTTTAATATCAAAGACAACAATTCTATCAAATTCATAAACAATACTATTGTTAATTTCCAGTTTTTTAAAAGCTTTTTTGATGTTAATTTTTAATTGTTTTGTGAAATAAGAACGGTTTTTTCCTTTTAAAACCAATTCACCATAACGCGCAACTAATACATCTTCACTATTCAATTCCATTTTCCTTTAATCATTGAATATCACTTTCTGTTAGTTTAGGACTTTTACCAGTTAATATGTAATTTTCATATCAAATTCTCGCCTTATCAACTAAAACACTAAATCCCTTATGATCTATCTTGATAGGAATTAACCTAATTTCTCTATTGCTAGCATTAAATTGTTCTGGTAAGACATAATCTTCTGGTTTCAAAAAGGCAATTGCAAAAAGTCCTTTATTTACATTGCGCAAATATAGATAAAGACCAATTTGACAATAATATAAAGTACTGATAATAATTTTTCCATTACTGTCAAATCAACTATCTTTTTTGCCATCTTTCTTTTTAACAATAGGCATACCATTTTCATCAAGAATCATCTTGAGATTTCCATTGATTTTTTTATAAATAAGACTATCGCAAGAAGTTGTTTTAATCTCTAACATAGGAAGATCATTTTGATAAGCTAATTCACCATTTTCATCAAGAGGTTCACCATCAGGTATCCCACCAAAAACACTATCATCCTTAAATAAATCAAATTGAACTTTTTTAGGATCATAAGAATGAAAATTAAAACCAGTTTTTAAATTGACATAATCACGTATTTTAGGTTCTATTATGTTTCCAGCTTTTGCCAGTGTTTCATCAAATTCATCTTCATAAATTTTGACCATATTAGCTCAAGTTTTAAATGATGACTCATATTCAGATAAACCTAAAACCTTGCCAAAGCGAGTACCGGTTATTTTTTTAAATAGAGCTTTATTTCTTAAAAAATACTGTTCTGATAGAACAATTTGATTATCAACTATATCAAAATCAGTTTTATATTGCTTTATAAATCCCATTTATTTCATCAACTGTTCACAAATTCCTTTTAAAACAATTGATATTTCAGGATTATTAAATTCCTCATCATTATCTGGTTGTTTTTTGCTTAAAAATACAACACTTGCTAATTGATTAATGTTATTAATTGGAACTTCTGTCACATTTAAATAATGAACATCTTTTAAAGAGCAATTCTTGCTTTTAACATATGTTTCTAAAGCACTAAACTTGGAATTTTTTGTGCTAGTAAATAAAAAATTTTCGTTATAAAAATTGCTGTAATGAAACTTAGGCTGACCACTAGAAATATGTTCCATAAGTTGAAAGTCTAAATGATACTGAGGACTAAATACATAAATAGAACTAACATCCATTTCACTGAGATTTTTTAAGAAATTTTGGTACTTTAATGTTTGGTCAAATTCCGTTAATCAAATTAATTTGAATTGCTTATATTTTTCTAATGAAATAAAGTAATTAATTGTTGTATTAGGTTCCTCACTAATCTTGCTTTTTCCAATTGCAAAAACACCAGAATTAGTAATAATAGCATGGTGTACAATAGTGTCTATACTAACAGAATCTAAAAAAGATTGATAGATAAATTCATTGGTTTTGCTAATTCATATAATCGCGCCTGATTCTGCAATTATATCAACATCATCTAACGAGAGTAAATCAAGTAAATAAAGCACTGCTTGTTGTCAACAACTTGACATAAAAACAAGTTGATGGTTATTCGCTTGAATGAAATTAAAAAAGTTTTTTCATACTTCTAAATCCTTTGTAGTGTTAAAAAAATTTGCTGTATTAATATCTAAAACAACTATTTTTCTATCCATCTCTTCTAACTCTTTTAACAAAATTATTAAAGCTAATTCTGGTGATTTTTTCACCTTTAAAACCACGGCATGTCAACTTCTCTAAATTCTCTATTTCTTTATCTCCAACAATAAGTTGTAAAGGAATTTTTTCAATGATTGCTTGTCTAACTTTTTTAGCTAAGCGATCTTGATTATCATCTAAATTTACACGGATGTTTTCTTTTAGCAATTTGTTATAAAGTTTTTTTGCTGCCTTTAAATGCTTTTGGATATTAACAGGAATAATTACGGCTTGAACAGGTGCTAACCATAAAGGAAAATTACCACTTGTTTTTTCAAGTAAAGCAGCAATAAACCTTTCATAAGTTCCAATAATTCCAACATGGATAATAACTGGTTTTTTTAGTGTATTTTTTTTATCTATATAAGTTAGATCAAATTTTTCTGGTAGTAAAAAATCTAGTTGAATGGTGGCAATAGTAATCATTTTTTTAAAGATTGTTTTGAACTGAAAATCAATTTTTGGTCCATAAAAAGCAGCAGCTCCTATCTCTTTTTGATATTGGATATTTAAATCTTTTAAAACATTCTCCATTTGGCTTTCAGATTCTCTTCATAAACCAGGATTATCAATAAATTTTGATTGATTTTTAGGATCATGTAGAGAAAGATCTATCCTATCAAATATAAATCCAAATTTTTTATTAACTTTTTGAATTAAATTAAATGCGTTTTTAATCTCACTTTTAATTTGATCTGCACGACAAAAAATGTGATTATCAAGTAAAGTCATGCACCTCACTCTTTCTAATCCTATTAATCCTCCAGAGGCTTCAAAACGATGCAAAATAGAATCTTCTGAAAAGCGCTGTGGCATTTTTTTATATGAATATCGTTTTTGTTTGAAAATCAGACAGTGATGAGGACATGTCATAGGACGAAGCATCATTGCTTGACTATCAAGTTTAATAGCAGGAAACATATCTTCCTTATAGTGCTGATAGTGGCCGCTAATTTTAAAAAGCTCTATGTTAGCTAATACAGGAGAACAAACAGTATTAAAACCAAACAATAGTTGCTGGTGATGCACAAAATTACCGATTATATTCCTTAGTGTTGTACCCTTTGCTAATCAAATAGGAAGACCTGCACCGATCAGTGGGTCAAAGCTAAATAACTCTAATTGTTTACCTAAAGATCTGTGATCCTTCTTCAAGCGTTCTTCATTTTCTTTTATTAATTGTTCTAATTCTTTTTTTGATTGAGCAAAAATGCCATTAATCCTCTGAAGTTGTAATTGTGAAGGATCTCCCAAAAAATAATTTACGCTTACATTTAATAGCTTAATAAAACTTTTTTTAATAAAAGTTAAAGTTAAATCTTCTATTCAAAAATGTTTATTTTCAAAAAAGGTGATTTTAAATTTGTTTAAGTTGCTTTTTTTTAATAAGTTTTTTGTAAACTGATCATTTTCAAAAAAACTTAATGCTTCATCTAAAGAAACAAATTTTTGAGAAATCCCTTCTAGTTTACTAGAAAGAGAGTTTAAATCAGATTCTATTTTTGCAAACTGTTTTGTAGAAAAATTCTCATTTATATAAAAATCTAAATAAAATTCATCTCCATTAAAACCATGTTCACCAAACTGAACATTAGCATATTTGCATTTTAACCATATTTGTAATAAAAGAACTCCTGCAAGCATTAAGATTCTTGATAATCAATCATTACTTTTGAATTTGGTTTCATAATTTTGACAACTTCGATTGTTGTTTTTCAAATTCCACTGCGTTTTTCTAGGTAAGTGCGCAGAATACCTTCGATGCTAATTGTTTTAAATTTTTGAGTATATGCTTCAAGTTCAAATGCTAATTGACCATTTGCATAAAAAACAAAAAAATCAGTAAAACTTCGTTCACCAAACAAACGTTTCTGCTTAATAGTTACTAAAAATCCTGTCTTTTTGTGGCTTCACTTTGTGCTTTCTATTTCACCTTCCAAAAAAACGCAATTTAACATGCTTTTTAGCTAGGTAATTGACCTGTTAAGTTGAAATGAAATTTAAAGTGGTTAATTGTTTCTAAAACCATTCTTTCTTCTATTAATGCAGTTCTAATACCTTCAAACTTTTGTTTATCATCAAGATATTCTCTAACATTCTGATTAGTTTTTTCATAATACAAGCTAATAACTCTTTTAACAATATCATCACTAACTTCAACCTTTCATTCCTTTTGTAAGAAATTAAATACTAAAGCTTTTTTAATGATTTTTTCAGCTATTGATTGAATTGTTTTTTCATCAGCATCTTTAACAACATCTCTTTTCAAGCCTTCAACAACATTTTTTAACTCTTCAGGGTTAATTTGAAATTCAAAATAACTAACAATTTCATTCATTGCTGCTGAAAACAAATTATCTTTAATAACAATATGAGTTAGTTCGTGGTAAAGAGCACTTTCCTTAGCATCTTTAAAAGAAGCTAAAATTCTTTGCCGATGTTGCTCAATAAAAGCAGGATCTGCAAAAATACGATCAACTTCAATTACCTTATCATACTGAATAGGTTTTTGCAACTTTGCAATAGATTTAAGATTAGTAGCCATATTTTTAGAAACTTATTAAATTTTAATTCCTATATGCGTGAAGAATTTACATTTGTTGTGGTGTTGAAATCTTCAAAACATCAAGGATTTGTTTAACTATAAATTGTAATGATCTCAATAAAGCAAGTCTTTGTGCTGATAAAAGTGAATTTTTATCATCATTAATTTTGCAAACTTTATACCAGGAATGAAATAAACTAGCAGTTTCATATAAAAAGTTAGTTAATAAGTGCAATTCGCCTGTTTCCATAGCTTTTTGCAACATAAAAGGATAATAGACAAGTTGATTTAAAAGTGATATCTCTTTTTCATTAATCAAAAGACTGCAATCAGTAATTTCTTTTAATTGATCAGAATTTGCAATTCTAAGAATACTATTCATTCTTGCAAACGCATATTGAACATAATAAACTGGATTAGCAGAGTTTTGCAAATTAGCTATATCTAAATCAATTTTGATAATTGTGCCATTATTTTGAGATAACATAAATCATCTAGCAGCATCTTCACTAATCATTGAAAGCATTGTTTCAATTGTGATTACATTTCCAGCACGTTTTGACAAACGTACTAGCTCTTTATTTTTATAGAGAGTAACCAGTTGTAATGCAAAAATTTTTAAAGAAGTTTTAGTGGTATTTTTTAAAGCATCAAATGCACAATACATCCTTTTAATATGTCCTTCATGATCAGTGCCTCAAACATTAAATAGAATGTCAAAGCCACGTTGTAATTTTTCTAAGTGATAAGCAACATCAGTTCCAAAATAAGAAGCTCTTTTATCACTTCTAATTAAAACTCTATCCTTATCATCTCCATAAAGCGTAGTTTTTAGTCAGAGTGCATTATCTTGATAAAAATAACTATTTGGTTTAAGTTGTTCTAAAAGATCATTAAAATTTGTTTTTTTAACAATTTCGCTTTCAAATTTTCAACTATCAATAAAAACATGGATTTTATTCAAATCACTTTTAATTAAAGACAAAACTTTTTGGGTGCAATTAACTAAAAAATGATCTAAGAATGATGTTTTACAAAACTCTTCAAAATTTAAATTATCAACTGGAAAATTTTCAATTTCACTAGCAATTTTATCTATTACTATTCCACTATATCCATCAGGATGTTGCTGGATAGCAATATTTTTTTTTAACTGCAAACTTTGATAAACACTAAAGCTAAAAACTCGTGCTTGTTGTCCATAATCATTTACCCAATATTCACAGACTGTTGTATAACCCAACAGCTTGGCTAAATTGTTTAAAACATCACCAAAAAAAGCTATTCTCACATGACCTAAATGGATCCTTCCGGTAGGATTTGCTGATACAGATTCAATTAAAATCTTTATATTTTTCTTAGTAAAGCAAGGTGTTTTTATCAAGTATTCTAAGTACTTTTGATAGCTAATTTGAAAGTTTATAAAATTATTGTTAGCAATAAAAACATTTTGGTAGTTATCCTTTTTATTTTTTAAAAATCACTCAACAATACTATCTGCAATTTTTTTATGATTTTCACTTTTTAGTTGGAAAATAATAGTTGAAGAAAAACCATTAAAACTATTATTTTTAACTAGTTTAACAAGTTCCTTTTGGTCATCAAATTTAAGCTTTAAAGCGCTAATGCATTCTTTTAAATCATTGATGATAAAAAACATTACTTTAAATTATATTTAATGATGCAAAATGTCTTTTATCATAACAGTAATAGGTGCTGGGCATGCTGGATTGGAAGCCGCTTTCATTGTAAGCAAATTCAACATCAAAGTAAACCTTTTAGTTCTTGATATAAATCATTTAGGTTCTTGTCCATGTAATCCTTCAATTGGTGGACCTGCTAAGGGAATTGTTACTAGGGAAATTGATGTTTTAGGAGGTATGCAAGCAATTGCTGCTGATAACAATGCCTTACAATATAAATTACTAAATAGTTCAAAAGGACCTGCTGTGCAAGCTATCAGAGCACAAATTGACAAAATAGGTTATAAAAACTGGTTTCAAAGTCAAGTTAAATTAAATAAAAACATTAATCTAATTCAATCTGAAGCAATCAATTTAATTGTTAGAAATGAAAAAATAAAAGGCGTTATTTTAAAAGACGGAAGTGAACTTTTAAGTGATGCGGTTATTATCACTACCGGAACGTACCTAAGATCAAAAACATACTGTGGTAATACAGTTAAAAATCAAGGACCTGATCAATCTAAAAATAGTGAAAAATTAAGCACAAACTTAATTAACAGAGGTTTTAAAACAATTCGTTTAAAAACAGGAACTCCGCCAAGAATTTTAAAAACTTCACTTGACTATAATCAAATGGAATTAGAAATTAATAATAATCAAAACCTTGCTTTTAGTACTACAAATAAAAATTTCTTACCACTTGAAAAACAAATACCTTGTTACTTAGTTCATACCAATCAAAAAATTCACGATCTAATCCTTAAAAACTTAAAAAAATCTGCAATGTTTAATGGTAGTATTTCAGCACAAGGACCACTTTATTGTCCAAGCATTGAAGACAAAGTTTTTAAGTTCTCTCAAAAACCTCGTCACCAAATTTTTGTAGAACCTGAATCATTGAGTCTAGATACTATTTATTTAGCAGGATTATCAACTTCTTTTACACCAGAAATTCAAAAAGAAATCATCCAGCTTTTACCTGGTTTTCAAAATGCAGAAATTAAAAAGTTTGGTTACGCTATTGAATATGATGCTTTTCTATCTAATCAACTAAAACCAACACTTGAAACGAAGTTAATAGAAAACTTGTATTTTGCTGGACAAATTAATGGCACTAGCGGTTATGAAGAAGCTGCTGGTCAAGGTTTGATGGCAGGAATTAATGCTGCTTTAAAATTATTAAAAAAACCACCATTTATTTTGCAACGTAATGAGGCTTATATTGGGGTTATGATTAATGATTTAGTTACTAAAACAATCAGTGATCCATACCGTTTGTTAACATCCAGAGCAGAATATAGACTATGATTGAGAAATGACAATGTTCAAGAACGGCTCATTAAAAAAAGCTTTGAACTTGGTTTAACAGATAAAAAAACATATGAATTGTTCCTTAAAAAGGAAAAGAAAAAACAGGAATTAATTTCATTTTTAAAAAACACTCAAGTAGGCAAGGTTAAAGCATTGAAATTCACTAATAAAAATACCGCTCAATCACTTTATGACTTCAACAAACGAAGTGAAATAAATTTAGATAAATTGATCAAAGATCTTCCTGAAAAATACCAATTAGATTCAGAAACACTTAAACAAATTGAAATTGAAATTAAATATGAGGGTTACATAAAGAAAAATGAAAAGTATTTTAAGGGTTTAGATAAATTAAGCAAAATTAAAATTCCTCATACTTTTGATTACCATAAGGTTAAGAATTTAGCTAGTGAAGCTATTTTTAAACTATCTAACTTTAAGCCTAGTAATTTAGCAATTGCAAGTCAAATAGCTGGAGTGAACTTTAATGACATTATAGCCATAAAACATTTTTTAAAAACTTATGAATAATGCTAATTTTGAAAAATATGTTGATTTAGTTTTTGAAGCAAACAAAAATTTCAACTTAACAGGATTTAAAACAAAAGAAGCTATTTATCAGAATTTAGTTATAGAAATATTGACATTATTTAAAGGATATGAAAAATTTTTTATTGACAAAACTGTAGCAGACTTGGGAAGTGGAAATGGTTCGCCTGGGATAATATTAAAACTGTTATTTCAAAAAATAAAAAAGTTAGTTTTAATTGATAGTAAACACAAAAAAATTAGCTTTTTAAATAAATTAACTAAGCAACTAAATCTGGAGAAAACTGTTGCAATTTGTGAACGAATTGAAGTACATAAAAATCACTATGATGTTATCTGTTCTCGTGGTCTAAGTACGATTATTAAAGTTAATGATTTAGCATTTTCCTTGCTTAACTCAAAAGGTATTATTTTTCATATAAAACAAAGCTTAGACCAATACATTGAATTTGAAAAATCAAATCAGAAGAATCAATTTAACTTGTTATTTATAAAGCACTTTACTAGTCAGAATAAAAAACTAATTTTGATAGCTTTACAAAAAAATGATTAAGTTAGGTTGAGCGTCTTTTAGTAATACAGAATTTGCTCAAGATATCTTTATAAAGTTTGCCAATCAATTCTATAAACAAGATGATGCTGGAACAATTTTATTTGAACTAAAAAAAACATTAGGAGTTAATCAACTAGATGAAATTGAAAAAAATAAAAAAGTTATTATTGTTAATCAGTTTCAAAATAGCTTAGGCAAATTTTTACTTTTTAATAAAGAAAATACTAAAAGAATAAACAGTTTAGCTAATGAACATATAAGTTCTTTTTTAAAAAAAATTTTCAGATTATCTGGATTTAAAGATATGTTGATTGATGTTCAACATCATAAAAAGTTACAAAAATGTTTATTAAGAGAAATTCATCTTTTAGTTTGTTTAATTAATAGCAATCAATTTAGTGATGAATTAATGCAAATTATTGAGTGGTATCAGTATTTAAAAAAACACTCTAGTAAACTTTTTGTTATTACTGCAAGTAGTGATAAAAAACCAGTAATAGAGCCAACTATAAATGAATATAAAGCTATTTTTGGAGAGTATTTATCTTCATTTCACCTAGATCTAAAAAATAATCAAAGTAATGATTTATTCCAGAAATTACTTGATCAGATCAAAATAAAAGCTACCTCAAAAACTAGTTTGAGATAGCTAAAAATATAAATGGCGCATCGGGAGGGATTTGAACCCACGACAACACGCTTAGAAGGCGTGTACTCTATCCACTGAGCTACCGACGCATTTCTTTACTAGAAACTATCATATGAATCTTCAATAAGACTCTAATAAAAGTTTAGATGATTTTTAACTTTTTTATTGTATTCATGCAATAAAAATTAATTTAAATAAAAGAAATTTATTAAAAAATCCTTAAAGCACAAATATCAATAATTTCCTTTTCTATTGATTCTTAACTATTTTTAAAAAAATAGTCAATTTATTTAGTCACAAAAACCTTAGTTAAAAATAAATTTAAGTTGCAATTGGATTGAACATATTATTTAAACTTTCTTTTAAATTAAGTGTTTTAAAAGTTGATTTTTAATAAATTAATTAACAAAATAAGATAAATAGTTAAAAACTCAATAATAAACGCTTTAAAATATTTCACTTTGATGGATGAAAAAGGGATTTTAGTTGCAATTAGTGGTGGTAGTTGCTCAGGAAAAACTACTGTTGCTGAAATGATTTATCAACTTTTAAGTAAAAAATTAAAAGTTGCGATCATCTGTCAAGATAACTATTACAAGTCCTATAAAAATAAGCCATTATTAAAAAGAAAAACAATAAACTTTGATCATCCTGATGCTTTTGATTGAAAACTTTTAAGATCACACATTGAAGATCTTCTAAACGGTAGTATAGTTAATGTTCCTTTATATGACTACATTAACTATACCAGAGCTAAAAAAACAGCAAAAATTGGTCCAATTGATGTTGTTATTCTAGAGGGTTTAATGCCATGATTTGATGAAAAATTATCAAGACTTTCTAAGCTAAAAATATTTATAGAAACAAATGGGGAAGAACGTTTAATTAGAAGAATAGAAAGAGACTGACAAAGGGGAAGAAATATTGATTCTATTATTAAACAGTGACGCGAAATAGTAGCACCAATGTATGAAATATTTGTAGAAAAAATGAAGCGAAATGCTGATTTAATTCTGCCTTGAAGTCAACGCAGAGAAGTAAGTACAAGTGTATTGGATGTCGCAATTGAACACTTATTTCACAAAACTGTTGAAAAAAATAATTAGTTCTGGAAATTATAGAGATGTTTAAATTTCAAAGGTGAATTATGTTTATGCTCTGCTTTTGCTTTCAAATCTAAAACAATTTTTTGCTTTTCTGAACTAAGTATTAAATCACCTTTTAAATATTGATCAAGTTCCTTATAAGTAATGCCCATCTCTGTCTCATCAGTTTGCCCTTCAAAAAGACTAGCAGTTGGCGCTCTTGTGATTACAATTTCAGGAATATTAAAATGCTTAGCTAATTTGTAAACGTCCTCTTTTAAAAGCCATGCTAAAGGAGCAATATCACAAGCTCCATCTCCTCATTTTGTGAAATAACCAAGTGTATACTCTACAAAATTACCAGTACCTAAAACTAAGAAGTTGTGTTTTTGAGCATAAGCATATAAAGTTATCATCCGTAAACGTGCTTTAATGTTACCAGCTGTTAAAAAATCTTTTTTTGGATCTATTCCAAGGGTTTTTACTAACAGATTGAAACTCTCTTCCAGTTCAATGTTAATACTATTAAATTGCATTTTTTTAACAAGTTCACTAGTTGCTTGAAAATCAAGTTTTGAATTATTAATATGCATTATTAAAGCTAAATGGTTTTCAAAACCAAAAGTTTCTTTAGCAATAGCAGCAACAACTGCTGAATCAATTCCTCCAGATAAGCCAAAAATAACACCTTTAGCTTTAGATTTTTTTACATAATCAAACAGCCAGTTTTGGAGTTCTTTTAAATATTTAATTAAATTAGTCATTAATTACAAATTCACAACCATCAAAGAAAATAATATCACCTTTTTTAGCACCAACGGATTTTAATTGATCTTCAATACCGATTTCTTTAATTTTATTTCCTAAGCGACGAATGTTATCAAGCGTAGTTTGTGGAATTTTATCAAATCAATATCTTAGACGTTTACAATTAACAATTCATCTGTTTAAACTATCTTTTTCAATATTTAAAGGGTCGTTATTATTTTCATTGGTATTTTGAAAAACATAATGCTTCTCAAGTTCCATTGGCAAACTAAACTTATTAGCTCCAAATTGGGAAATTGTTTTATTGTAAAGTTCAAAAACTCTATCAAGTAAATTGCCTAATTCTTTCTTTAAAGCAGAAATTTTTAATACAGAAATAGATTTTTTTTGAAGAAATTTTTCTAATTTTTTAAACCGTTTCTCACCTTCATTAACATCAATTTTATTAGCTACAACAAGCATCTTTTTTTTAACTAGAAGGGGAGAATATTTTGAAAGTTCATCCATTATTTGTAAATATGCTTTGCAAGGATCATCATTATCTACAGGATCAAGTGAAATTAAATGAATTAATATTTCACACCTCTCTATATGCCGTAAAAAATCATGTCCTAACCCACTTCCTTCACTAGCATTTTCAATCAAACCAGGAATATCAGCAAAAACTAAACTATTATTTTGATATTTAACTACACCAAGTACGGGTATTAAAGTAGTAAAGCGATAGTTTGCAATTTTAGGTTTGGCATTTGAAAGTTTGGAGATTAGTGTTGATTTACCTGAATTTGGAAAACCAACAATTCCTACATTAGCTAAATATTTAACTTCCAAACTAACGTTTAAAATCTCTCCTTTATCACCATTTTCATATAGATTAGGAGCACGCATAATTGGGCTTTTAAAAGCAGCATTTCCTTTTCCGCCTTTTCCGCCAAAACACAAAATAAAACTCTGTTTATCATGAACAAAATCCACCAATACACTATTATTTTCTAAGTTTTTAACTGTTGTTCCTATTGGCACTTTAATTAAAAGATCTGAACCATTTTTACCATGTGCTAAATCTGGTTTACCATTTTGTCCATCTTCTGCAAACAGGTGTTTTTTATTCTTCAAGAAAAAAAGTGAATCGCAGTTATGATCAGCTTGCAAAATAACATTACCACCATTACCACCATTACCACCACCAGGACCACCTTTATCATAATGTGCTTCTCTTTTTCATGCTATGATTCCATTACCACCATTTCCAGCAGTAAAGCGACACTCACAATAATCTGTAATAGCCATTATTTTGGTTTCTTTTTCTTTATTTTAAAAACGATTTCTGCAGATTTTTTTCTTTCTAGATCACTTTGAATTTGTTGCAACTTTTGTCGATTTTTAGTTTTAGATTTACCTGTCTTTAAGAGATTTTCAGCAATTATTATTGCATATTTTGATCTGATTAAAAAAAAGTTAGTCATTTTATTCAAAAGAAAAAAACTTAGCATTAATGTTATTAGAACTAAAAAAAATCGAAGTAAGATAATTCAACTATTTCAAGCCATTGGTATTATTCCTGATAAAAAAAGAACTATTAAAGTAAAAGCAGTAGAATAGCAAAAAGGAATTAATTCCCAAATAATGTATTTAGTAAAAGCAAATAAAAAAAAGGCTAAAAAAAGATAAATTAGTAAAGGAACAAATAAAAATCACAACAAAACTAAGTCAAAAATGGGCTCTATTTCCTTACCCATAAATGTTCTGTTTTGTGCTGCAATTAAAAAATTAAAGCCTAGTAAATTGATTTCACCAAAAAGAATTCAAATCAAAAGATAATAAATAAAAACTAAAAAGAAACTAAAACTTCCTATTAGTCAATTAGGGGTTTTAGTTTTTTTATTTGACTTGAAAAAATAATTCAAAACTAATAAATTTGTGCAGTAATTTGAAATTTCACAAAGCGATCGTTTTCATAAAATTGATAACGAAGAAGACCCTTGATAACTCAAAGAATAACTGGTAATCCCAGGTTTTGAATTTCTTTGCAGTTCTTATGATAAAAACTCTCGTTTGGCACTAAAAAACGGCAATATCTCATTAATTCGTCTCTAGAAGCTACTAATAAAGCTTTTTTGTTTGCAATTACAAAACCGGTTTTAAAGCCTTTGAAAGTCTCATTTATTAACTTAATGATATGAAAATTACTACTTAACAAAATCATTTTTTCTTTAGCTAAGACAAAATCTTTGCAAAGCAAATTCAACTGTTCTAATAATGAATTGTTATTTTCTTTATCATCTTTAATATGAATCAGCAAATATTTAAAGGGCTGAATAAATTGCGTTAAAACTTCCTTTAAAGTTTGAATTTGTTGTGAAGTTACCTTAAGCTTAAATTCACTTGCAAGGTTAACCTTTTTAAGATCAACTAAATTAATTTGGTTTAAATTCAAGTTTTTATTAGAAACTTTAAAGTTTTCTTTATGAGTTACTACTATTTGTTTATCTTTGGTTAATTGAATATCAATTCAAATACCATCAAAATCAAAAACTTGGGCTGCTTGAAATGCTAATTTGGTGTTTTCTGGTGCAATAGAACTATAACCGCGATAAGCTATTAAAAACTGTTTTCGCATTACTATTTTTTATCATTACTGCCAAAAACATCCTTAGCATCATCTGCTGAAAATTGACTAAGTCATTCATCTGCGTCAATAGAGTCAATATCATAACCTTCAAGAGCAGGAATATCCTTAGTTATATCTTCATTTAAATTCTCAGTTTCCTCAGACTGGAATACTAATCAGTTACCTTCACTATCAAATTCCCCATCATATCAGTTTCATTCATTGTCTTCATAGTAACCATATTCTTCATTTCCGATTAATTTCTCTCAAAACTTAGGTGCCTCGTTAATTCGTGGTTGACGATTCTTTCATTGTTCATCAACAAGAACTCAGTCTCCAGATTCATCATAGAAACCATATCAAATCCACTCACCTTTATTGTTGTACCTACCATAAACTTCATCACCAATAAGTGGTAAATAATCTCTTTCTCTAGCTCATTTAACAGTTGCATCCTTATTTCATTTTTGATCATCACCAAAGAATCCTGCTCAAACTCATTCTCCATCAGGTTCATAATGACCATAACTATTGTTACCAACTAATAATTCTCAATCAAGTGATTCTTCTTCACTAGCTTCTGGTTGAATTTCATCAGCTTGAGGTTCTTCCAATACTGTTTCAGGTTTTCGTTCTTCAAAGTTAGGTTGAACAACTACTTCAGGTTCACTAGAAACTTGAGGTTCTTGAATAGTTTCAACTGGTGAATCAAATTTAGCTTCTTGAGGAGTTTGTTCAGGTTGAACAGTATCAAAAGTAGCTTCTGGTTGAGATTCTACAGGTTGAATTTCTGGTTGAGTTTGAACTTCTTCAAGGAGAGGTTCAGGTTGTGAATCTTGAACCGATTCAACTGGTGAATCAAATTTAGCTTCTGCAGATGGTTCAAAAACTGCTTCTCCAGGTTGTTGTTCTACTTGAGGTTCAAATTGTGGCTCTGATGGTTTTGATTCAATTTCATAATTTGGTTCATCAAAGTCATAATTAGGTTCATCTACATCATATTGAGGTTCTGATGGTTGTAGATCTGAATCATAACTAGGTTGATCAAAATCATAGTTAGGTTCATCAAAGTCATAATTAGGTTCATCAAAACTAGCTTCTGATTCAACATGTTGTTGAGTTGGTTCACTACTAAATTCAGGTTGTTCAACAGTTTCAACTGGTGAATCAAATTTAGCTTCTTGAGGAGTTTGTTCAGGTTGAACAGTATCAAAGGTAGCTTCTGGTTGAGATTCTACAGGTTGAATTTCTGGTTGAGTTTGAACTTCTTCAAGGACAGGTTCAGGTTGTGAATCTTGAACCGATTCAACTGGTGAATCAAATTTAGCTTCTGCAGATGGTTCAAAAACTGCTTCTCCAGGTTGTTGTTCTACTTGAGGTTCAGAAATAACTTTTTGTTGATTTTCAACATCATCAAACTTAACTTCAGTAGTTTTTTCAGGTTGAACATCAAAAACTGCTTCAGGTTCTCCAACAGTATCAGTAGTTGTTTGATCAACTACTTCAGCTTCACTAGAAACTTGAGGTTCTTCCAATCCCTCAGTTTGAGTTTGATTTTTATCAAAAACTGCTTCATGTTTAACAGTATCAAAGCTAGCTTCAGTTATTAATTGATCAACTGGTTCAGATTTAGGTTCCTCTTCTAATATTTTTTCAGTCTGTGTTTCTAATTGAGGTTTTTCAAAAACTGCTTCTGATTCAGCTTCAGATACATTATCAAAAGTAGCTTCAGATTTTAATTCTTCTGTTGATAATGGTTCAGAGGAGGTTTCCTGTTGAAGTTCTTTAGTTTCATCAAAAGTAATTTCAGCTTCTGTTTCTTTAGGTTGAACTTCATTATTTTCAACATTATCAAAGTTAGCTTCCTCTAGTTTTAAATCTTCTAACTTAGTTCCATCAAAGATTTGTTCAACTTCAGCAGTAACAATTTCTGGATGATTTTGATCTAATGCAGGTTCAGATGGTTGATCTTCAGAGCTAGGTTCTAATTGGGTTTCAATTGTTTCTGGTTCAAAAGCAGTAACTTCAGTAGGTGTAACAACTGTTTCTTCAGACTGATTTTCTAATTGTTGATGATCAAAAACTGCTTCAGTTTTTTCTTCTAATTTAATTTCATCAGAGATTTGTTCAGCATGAAATTCAGCTTGCGATGATTCTTGGGGAATTTCGACTGGTGAATCAAATTTAGCTTCTTGAGGAGTTTGTTCAGGTTGAACATCTGGTTGAAAATGATCATCAAAAACAACTTCAGTAGTAGCTTCAGGCTGTAGTTCTTGAGTTTTAATTTCATCAAAAACTGCTTCTGGTTGAACAGTATCAAAAGTAGCTTCAACTTGTGATGATTCTTGAGGAACTTCAACAGTTGAATCTAATTTAATTTCTTTGAAAGTAGGTTGGAGTTCTGAATCAGAACTCTCTTTTTCGTCTGGTTTTGAATCAAATAAAGCATTTGATTCAATGATTTCAGTTAAATCAAGCGGTAGAGGAGTGAATAAGTTATTTTCATCTTGATGTTCAAATAATTTTGTTTCTTGTTCAACTGTGGGTACATTTGATTCAGAAGTCTCAACCTCTTGAGTTTGACTAATGAATTCAAGCGTATCTTTTTGTTCAGATGAAAATTCATTAGGAGTTTCAGCACTTTCAGTGAAACTGTCTTCAACTATTTGATATGTTGGTAATACGAACTCACTATCAGTTTCTAGTTGTGTTGTTGGTTGTTCTTGAACTTCTTTTTCAGAAACATCTAAATTAATAAATTCATTACCAATTTCTTCATGATTTAATTCAACAGATGTAGTTTTAGTTTCTAATGATTTATCAACATCATTTACAGAATCTAATTCAAGTGATAGGTCAGGTTGAATGAAAGGTGGTTCACTTTCTACAACTATTGTTGTTTTTTCATCAAATAATGATTTTGGTTCAGTTTCAAGGAAATCTACTTTATATTCCAATCCTTCTTGAGGTTTTGGATCATTATTAATAAAAGTTATTTCATCAACTAGTTCACTTTTGGGAAGTTCACTTGAAACCTTAACTTCTGTTTCTGTTGAAAAAGGAGCATTAACATCAACATATTCATTTGTTAAGAGTACCTCATTAACAAGTTTTTCACTAAATAAAGCAGTTAAATCAGTATTAACACTTTCATCACTGTTTGTTTCTTTTATCTTTTCATCTGAAACAACAGGTTCGCTAAATTCTTGTTGAAATTCATTAACAGAATAAGTAGGTTCAAATTGTTGTTCTGAAACTGTTTCATTTATTTGAGAATCAATATTATTAACAACTTCATCTCTAAAAAGGGATTCATTAATGAAATCATTATTAGCATCTAAAATAATTTCATCTACTTGTGATTTTTCTTCTACACTTTTTTGTGTTTCATCAGTAAAAGAACCGAATTCTTTTCAAACTGAATCAATATCATCAGAAATATTAGATAAATCTGCTTTAGCACTATCTTTTATTTCTTTAGAATAATCTTGACTTAAATGTTCAACATGTTTAGCAATTTCATCATGTTGTTGGTCGTCTTTAATAATAGAATCTAAGCCTTTAAGATCATCTTCGTAAACAAAAACGGTCTCAACTTCTGGAACATTTCCTGCTTGAGTTTGATTGGAATCTAAGCCCTTAAGATCATCTTCGTAAACAAAAACAGTCTCAACTTCTGGAATAGTAGTTTCAGGTTCACAAGCTGCTTCAGGTTCAATTTCAACAGGAGCCTCAACTTCAGATTCAACCTTAACTTCCTCTTTAACGATTTCAATTAAAGGGATGGTTTCATTAGTTTCTTTAACTGGATTTAAGAATTCAAATTTTTCTTTTTCAAAATGTAATTTAGTTACTGGATTACGGAAGATAACTTCATTTCTTCTTGGTTTAACTTCTTCAATTACCGGCTGCTCTTCACTTGAATTTTCAAGTAATAGTTCGTTTTCTAGAGAAGAAGCTAAGCTCTTGGTATTAAATACTGTAACAACAATGTCATTTTCCTGTTCTCAGTATGGTTGTTCAGAAATAGTCTTTTTCTCTATTTCATCAACTAAGCTATCAGTATGACCAAGTGCTTCTTCAGTAAAGCGAACAGGATCAGGGATCCAATTTAGTTTCTTGTCAAAAAATCCTTTTCAGATCCATGTATTATTTTGAACAAAACCATAGTTAGGGTTGCCTACAAGTGGTCTTCAAAAATCAATATCAGCTGGTCTTTGTTTAATGAAAGCTGCATGTTCTTGAGGAACTTCAACTGTTTCAATTACCTTAAAGTAGTTTCATCTTTGTAATTGGTCAAAATAACCTAGTCAAACTCAAGTATGATTTTTTCAAAAACCATATTTTCTATCACCAACAAAGATATATCAGAACGCTTGTGAATCTTCTGGATTTAATTTTTTTTCTCTAATAGTTGTTGTAAAACTATCTTTTTTTGTTTCTAGAACTTTGGTTTCTGGTTGATTGTTTAATTCAACCAACTTATTTTCATTAATGGTTGGATAACTGCTTGTTTGATTTGGAACTGTATTAACAACAGTTTTTTGATTAAGTTCTCTTCTACTTAAGATATCTGTTTCAGGTGGAACAATAAAAACACCGCGCTTGGTTTCATCAAAAAAATCTATAGGTGATTTGCTCTTTGATTTAACTGTTTCCTTTTTCTTACTATTAAAAGACTTTGTTTTCTTGAGAATGTTTTTTTTGATTTCACCTAAAAAAGGTTGATCAAAAAGATTTTCAACTTTATCTTGTTCTTTTTTTGGTTTAGTAACACTCTTCTTGTTAGCTGATTCTAAATACTTTTTGGTTTTTGATTTAGGGGTGGTGTTTTTGTTTTTATTCTTTGTTGTTTTTGGCATTAATAAAAATACTTAGCACTAGTGACTAAAGTTATCTGACTCACTAAATTGTAATTTTTAATATCAAGATAACAAAAGTTAGCTATATATGCTAAAAAATAAATAGATTTTAATTACTATTTATATTAATTTATTTAATGTAGATTTAATAGTGTTTCAGCTTCTTTTTGCAAAAGAAAAATAGTTATAAAATCAAAAATTAATCACTTTAATTTCAAAAAAATTTGACAGCTAAAGCTTTACATTTTATTAACAACAGAACAAATAATTTTTAGCTTTTAAAAATTTATTTAGAAAGAAAACTATAAACAGGCAATTTTTGTTTTTCTGTTTTTACAAAAATATCAATAAAAATATCACAGTCAAAATGATTTAATAGTTTTTTGCGAGTTGCTATCCCAATTGCTTTGATCATTTCTGCATTTTTTCCAATGATAATCTTTTTTTGGCTTAATTTTGGAACACTAATTACTAGGTGAATTTTTAACAAGTTTTTTTCTTTATTGAAACTCTTTTCAATAATTTCAATTCTTGCTATATGGGGAATTTCGTTTTTGCAATAAAAAATTATTTGTTCACGTAATCCTTCAAGAATCTTAAAGTCATCGTTGTTTGCATCAATAAAATTGATATCTTTACGAAAGATATTATACCTAAGTTCAACTTGTTTAAAAATTGATCAAAAGAGGTTTTTATCAAACTTAAGCAAGTTTATTTCAATTGTTTTTTGTGGTTTAAACTCTTCTAAAATAATTGCTTTATTAACTTCACTTAAACTTTTTTGATGAAATTTATTAATTAAAAAAATTCTTGTTAAATTTTGATAGCGCTTTAATTGTTGTAATTGAGTTTTTAAAAATTCTATCTTGTTATTCTGATCACTTCTAACAACTAAAAGTAATACATCAATTCCACTTAATGCTTTGCGAATTTCTTTAGTTATTAATTCATAATTTGAATGTTTTTTCTCAGTAAAACCAGGGACATCAATAAAAACAATATTTTTATTAGCTTGGTTAATTACCTCAGTGGAAATAGAAAGAAGCGTTGTGTTATTCATGGATGATACCATCAGACTATCATCGTTATGTAAAAAATTAATTAGCGTTGATTTTCCTGCATTAGTAGGTCCTAAAACACCAACTTTTAAAACTTTCATACTGATAAGATCTCTTCATGAATTGCATCTTGAAAAAACATAGTAACGCTTTCAAATGCTTCACTTTCTTCATGATTAAATTCAAAGAGGTGTAAAATGCCGTGTATTAAGCATCTAGTTAAAAGAAATCAATTGTTACAACCAATCTCTTTAGCTTTTTTAAAGATGTATTTAGGACACAAAAAAATTTCGCCGATTGCTAGTGAAAAACCGGTTTCAGTTTCGTTATAGCACAAACTAATTACATCAGTACAACCTCTACGTTTTAAAAACTGTTTGTTCAGTTTTTTCATTTTTGAACAGGAAACAATTAAAAGCGCAAATTCTGGGTCAAACGATAGGTTTAAACTGCTTTTAATAATTTGGAAACAAAGTTCGACTCCTGCTTGGTAATTTTTACCAAAAAAGCGCTTAAAAAGGCGGCTTGAATTAATGCTAAAAGATGACTTCAAACAAATCTTTTGATGGTAACATGATAGTAATATTATATCAGCCCTGTTCTAGAGTAACACTTAAATCTACCTTTACTAGATTTTTGTTCAATTCTGATGAAATTTCTGATGTAGAAATTTTCTTTTTGTAAAGTCTAAAAGCTTTTTTTGCTGATTTTTCTGTTGTTTCTGCTTGAAAATTAGTCCATGAAACGTTGTTAAAAAGATTTTCATCTTTTTCATTTTTAAACATTAAAAAATTTTGTTGTTGCGTAGTTCAAAGATTTACATTAAGATATTGTTTTACCTTTACTGAAAACAAGATAGCAACTGTTGTTAAAAGCAAAATAATAAAAGCAATTGTTATTCCTAAAATTTTCTGCTTCATAATTTAACTATTTCTTAAGTAATTAGTTAGGTTAATCTTGTTTTGAGCTTCACTTGCAAAACTCAGATCATGCTCAACTAAGACTACAAAATTATTTAAAGTAAGTGGTTTTACTAATAGCTGATAAACACGGTTTTTAATTTCAGGTGCTATTGCATTCATGCATTCATCAAGTAAGATCACCTTATTCTTAATGTTGCTAAGTAAGAATAGTTGTAAAATTTGTTTTTGTCCTGAACTTAACTTTGTTTGATTTTGAACACCTGCATCAAAAAGTAACTGATAGATATGTGGATTCTGATTATTAAATAAAGCCTCACTAAAGCCAAAATCATTTGCTTCTGTTTGCATTAAATTTTGACCCTTTAGATAATAAACGTTTTCAAAAAGCTGTTCTTTTGAACATCTTTTTAAATCGACGTTATTAAATTTAATATTGCCCGTATATTCAAAACCTCTACCAGTTAATATTTTTAAAAAAGTAGATTTACCACTACCATTTTGTCCAAAAATAACTGTTTCATTTTTGATTTTTAAACTAAGGTTAGCTAAATCAACGTGTTGATTAAGTGTAACTGATAAATTTTCTAGACTAATTTCATCAGGACACTGTCAATTTACTTTAATACCCTCATCCTTTTTGGTTTCTATAAACAAGTTAACTAATTTCTCACGACAATAGTGATAAATGTTTATTGCAGCACCAAATTGAACAATTCTTGTAGCATAAGTTGAAAAAAGTGATTGGATACCAAAAACATAGAATAAAAAGCTCAATTGATAACGCTGTTCAATAATACCAATCACCCCCAAACCTAATATAGCAAAATCAAAACCTTGTTGGAAGAAGCTTTTAATGTTTTCTATAGTTAGACGATTAATTTCCTTCTGGTTGTTAATGTTTTGATTTTGTAAAAGTGCTTTACGAAAATTCAACATTAAGAAATTAAAGCGCTTTTTATTTTGTTCTTCACGCAAATTTTCATCTAGCTGTAAACTAATTTTGTTTTGCAATTCAACATAAGGAATTTCCTTCTTTGTAATTTTTTTAGTAAAAAAGAAGTCATAACAAAATAAGCCAGCATTGACTACTATTTGTGCAATGGCAATCAATAAAAATTCTAATTGATTAATTCCTATTAAAACACCGATAATTAGTGCAACAACACAACTAATAATTAAATTGGGAATATAAAAGTTAAAAAAGGAAAGAACTTCTTTTAAATAAAATTGTCGTTCTATTAATTGATTTGGTGATTTATTACTAAAGTAAACAAAGCTTTTTTGTTGCAAACTTTGAATAATTCAACCCAAATATCCACGGTATTGTTTTCAAAATAAAAAATTCCTAATTAGTTGCAAAATAACTTGCAAAAGCAAGTTTAATCCCTTTAGACATGAAAAATAAACTACCAAAACAACTAAATTAACTGCAGTTCCAAAATCAACAGTATTAGTTATTATGGTACGCGATGCTGTTGCTAATAATGTAGAGATACCAATAATAATCAGTTCAATAAAAACATAAAAAGTGACTAAATTAAAATCAAAAAAGTTACTGAAAAAATTACTTTTATTTACTGGTGGAATCTCTTTAAATGCTTTTGCAACAGTTGCTGCATAACCCGTTCAGAGTTTTTCTAGCTTTTCATCAGTTAAAAGGTATTTTCCTTTTGCAGGATCATATACCTCTCAACTATTTTCATGTTTGTTTTTAACTATGACAAAGTGATCTTTAAAATGAACTATTATGAATTTGCTATCAAGTTCTTTTAGTTCTTGAAATGTTAATTGATAACTGTTGATTTCAAGACCAAACTTTTTGCCATAACTTTCCATTTCAAATAAGCTTAAGCCATTTGGTGGTAAGTTAATTTGTTCTAGTAATTCATCATGAACATACTTTTCATCATGGATTGCATTGGCAAGCATACCAATTACGCAAATTCCACACTCATTCTGTTGTTCTTGATAAATGATTTTCATCGCCTTTGTTTTGTCTTATTAATAGCAATGAAAAGTGAAAAATGACAAATTTGTAAGCTGTTACAAACACTACAAACTGTTTTATTAGTTAAAAATAAAGTTACTTATTATTTATTTGCAAGCTGACTAGCACGTTCGTACAAGGTTCTCACTAAAACACCTTGTCCAAGGTTCTCAATGGAAGCAGTTGATGCAATATCACAATGGATCAAAGATACACCTTCTCTAAATTCTGCAAGGAAACAAGCTGCTCTTGAAGATCCAGCACCTCTTGCACTAGTAGAATTTTGCAAATCAGCAAGTTTTGTTAGCTGTAAAGGTTTTAAATAATCAGGGTGCATAGGTAATCTTCATACAGGTTCACCTGCACTACATGCTGCTTTTTTAAAAGATTCTCACTGGTGATCACAAGTACTGAAAATACCTGTATAGGTAGTACTCAATATGTATGACATTAAACCAGTAAGGGTTGCTACATCAATAATATGTGTAGCAGCTAAATCCTTAGCAGCATATGTAATAGCATCAGCTAAAACCAATCTTCCTTCAGCATCAGTGTTATCTATTTCAACACTTTTACCATTGTATGCTATCTTAATGTCATCAGGACGTTGCGCCTTAGCACCAGGAAGATTTTCAGTAAGAGCTGCTACTGCAACAACATTGGTTTTAACCTTGTTTTTAGCTAATGCCAAAACAGTAGAACATACTATAGCCGCACCACTCATGTCATATTTCATGCCACGCATATAATCACCTGTTTTAATGTTCATTCCGCCTGAATCATAAGTAATTCCTTTCCCTACAAAAGCAAGTTTTTCTTTGGAATTTTTATTAGCTTGATAACTAATAACAAGTAAACGTGCTTCTCTTTCAGAGCCTTGATTAACCCCTAAAAGTAAACCCATTTTTTTCTTAATTAGATCTGATTGTTTAAGAACTTTTATTTTTACAGGCAATTTACTTGCAGCCTTTTCAAAATGTTTAACAAATACTTCTGAATAAAGTAGATCTGAAGGAGTGTCTTGTAATTCTCTTGCTAGGTTAACATATTCGCCTACTGTTTCATATTCTTTAACTAAGTTGTGATATTCAACAGCACATTGAACAGCAACTTCAACATTTTTTTCAGGTTTGGTTTTCATTGTGAAAGGAGTAGTTTCACAAAAAGTAACACTTGTAATAACAGTTTTTATCGCAGTATGAAAACAACCACTATTTTCATTTTTCTTATAAAGTTCTATAAGACTGTCAAGATTAATGTTTAATTTGGTTTTCTTTCTTTTAAGAAATTGAACTAAAGCACGGTTGAAAGCAGGAAAATCAGTTGGTAAAACAACACCAAATTCAACATTAGGATTATGTGCAGCTTCTTCTTTTACACCATATGTTTTTGAACTCACAAAAACAACTGTGTTGCTATCATCACTAAAAGGTTTATTTATTCTCATTCTATATCTCTATTTATTATTCTGAAAAGGAGTTATCTTTTCTCTCTTTAACATCATACACAGCAACATTAGTAGTAATTAATGAACTAGCTACAGAAGCAGCTTTTTCAAGTGCAGTTTTAGTTACTTTGGTGGGATCAATGATTCCATTTGCAATCATATCAACCTTCTTTTTAGTCTCAGCATCAAAGCCAACACCAGTTTTTTCATTTTTCAATTCACTGAGAATTTTAACTGGGTCAACTCCTGAGTTTTGAATAATTTGACGCGCTGGTGCTTCTAGAGACTTTTGCACAATTTCAAAACCAAGTAGGATTTCTTTAATGTTTTCAACACTAGTTTCATTTTCATATCGTTCTTTTAGTTTACTGTTAGTTAAAACACAAGAAGCATTTAATAAACCAACACCACCTCCAGCGATAATCCCTTCTTCAACTGCAGCTTTGGTGGAATTTAAAGCGTCTTCGATTCTGAGTTTTAATTCTTTTTGTGCGAGTTCAGTTGCACCACCAACACGGATAACAGCAACACCTTGACTTAAATAAGCAATTCTTTCTTTAATTAAATCAGAATCATACTTGTCAGTGGTTTGTTTTAATCTTCCGTTTAGAAGTTCAACATGCTTTTTGATTTTATCCTTATTGCCTTTACCACCAATAACAGTAGTTTTTTCTTTAGCTATTTGAACCTTTCTAGCATCACCTAAATTATCAACAGTAACATCTTTAAAACCACTATTAATTTCGTTATTATATGCTAAGGTTCCACTACTAATTGCTAAATCTTCTAAAGCTGCTTTTTGACGTTCACCATATTCATTACATTTAACAGCAACAACATTAATGGTGCCCCTGAGTTTATTAACTGCTAAAGTAGTAACAACTTCTTCTGCAAAGTCAGGTGCAACAATTAATAATGGATTACCATTTTCAACACTACCTTCTAAAAGCGGAAGAATTTCTTTAATTGTGTTAATTTTTAAAGAGCTTACTAAGATTTTAGGTTGTTCTAAAACAACTTCCATTTTTTCTTGATCACTAACCATATAAGGTGATGCATATGTTCCTTTAAATTCAATTCCTTCAGTGGTTTCTAATGTTGTATTAATGGTTTTTGCATCATCAGTTGTTATCACGCCATTTTTACCAACTAAAGCCATTGCTTGAGCGATCAGTTTACCAATTTCTTTAGAACCTGAAGAGATAGCTGCAACTTGTTCTATCTCTTCGTTAGTATTAATTTTTTTAGAGTACTTTTCAAGTTCTTTAATAATAAGTAAGCTTGCATCTTCAATACCCCTGCGGATGTTAACAGGATTAGCACCTTTATTGATAATTTCAATACCACGGTTTGTCATTTCTTGTGCTAATATGGTAGCTGTTGTTGTACCATCCCCAGCAATGTCATTAGTTGACACTGCAGCAACTGAAATAACCTTAGCACCAATATTTTCAACTGGATCACTAAGTTCTATTTCTTTTGCGATTGTAACCCCATCGTTAGTAATTAATGGGTTTGCAAATTTTCTCTCTAAAATAACATTTTGGCCTTTAGGACCTACTGTTACTTTAACAGCATTTGCTATCTTATTAATACCCTGCAACAAGCGGGTTCTCGCATCTTTACCAAAGATTAATTCCTTTGCCATAATTGTTTAATTTTATCTTTTTCTTTGCTTACCACTTTCTGGTTTTTCAAAGGCAAGTACATCCTCAAATCCAATAATTTTGTACTTGTTTCCCTCATTCTCAAAAGAGATACCACTATACTCCTTAAAGTAAATAATATCACCAACACCAAAAGCATATTTTGGTTTTTCTGTTTTGCCATATGCAGGACCAGCACCAAGAGCAATTACAATCCCTTTATTAGCATTAGCATCGCTTTTATCATTACTTGCCAATGAGGTAATAATCCCTTTTTTTGAGACTTCTTCTTTGTTTGATTCCACAAGTGAAACCAAGACGTTGTCATGAATTGGCGTTATGTTCATAATAAAAATATTTTAATAATTAAAAACTAATTTAAAAGAAAGTAAAAGTTATCTTAAATTTATCTGATATTAGCGCTAAACATTAATTAATATTTAGTGAGATATTGTTAATTTTTAAATACAAATTAATAAGCTAAACCCTTATAGAGCGGAAAGCGTTTTAAGAGATTTAAAACAGCTGTTTTTGTTTGACTAATTACCTTTTGATTACCATTACTTTTAATAACCTTATCAATCAAACTGGCAACAAAAATAAAGTCATTAGTTTTAAAACCTCTGGTTGTCATTGCAGGAGTTCCAAGTCTAATACCTGAAGGACTAAAAGCAGATTTTGTTTCAAAAGGGATTGTATTCATATTCAAAACAATGTTAGCTTTTTGTAACCACAACGCAACATCTTTACCATTACCAACCACTAATGAAAATAAGTGGGTTTCAGTACCTTTTGACACAACACGATAACCCTGCTTTAAAAATCAATTTGCCATTGCTAAAGCATTATCTTTAACTTGTTGCATATACTGCTTAAACTTTGGATTCAAAGCTTCTTTAAAACAAACATATTTAGCTGCTATCACATGTTGTAAAGGTCCACCCTGACATCCAGGAAATACTCCACTATCAAGCTTTTTGATAATTGCTTGGTTGTTAGACATAATGATACCCCCCCTAGGACCACGCAAAGTTTTATGAGTTGTTGAAGTGACAACATCCACAAAAGGCAAAGGGTTTTGGTGCAAACCTGCAGCGATGAAACCAGCAATATGGGCAATATCAGCTAAAAGATACGCATTAACTTGTTTTGCAATTGCACTAAATTTTTTAAAGTCAACAGTCCTAGAATAGTTAGAAAAACCACAAATAATTAACTTTGGTTTGTGTTCGAGAGCAATTTGAAGAATTGCATCATAATCAAGAGTTTCTGTTTCAAAATCTAACGAATAAGTTACTGCTTGATATTGCTTACCTGAAAAATTAACGGGGCTACCATGGGTTAAATGACCACCACAATTAAGATCTAATCCTAAGATAGTATCTCCTGGTTTTAACAATGCTAAGTAAACTGCATAGTTAGCAGATGATCCAGAATGAGGTTGGACATTAGCCCATTGTGCTCCAAATAAAGTTTTGCAACTTTCAATGGCTAAGTTTTCAGATTCATCAACAACTTCACAGCCTTGATAAAAACGTTTACTGGGATAGCCTTCTGCATATTTATTTGTTAATACTGAACCAGTTACAGCTAATATGTCTTGGCTAACGTAATTTTCTGAAGCAATTAAACAAATGTTTTCTCTTTGACGTTGTAACTCTTTATTAAGTAAAAGTCTTACCTTTGAAAACATTCAACTTAATTAACGTAATAAAAACGGTGTTTTTTGATTGTTCTTATCAACATATTTAAATTCTTCCTTTTCACTACTTTTAATTTGGTCGTACAAGTGGGTATTGTGCTTTTTAGCAAATTGTGCATAGAAAGATTTTGTGTTACTGTCACCAAAAATTAAATCATAGTGCTGAAATTTATCCCTATTATTAGTAGTTCTGGTTTTATTTTTTTCACTTTCACGTTTAGAAGTTCTTAAAAGTTGAGACATACCAATGTTATCTTTATGTGTTTTGGAATTCTTATATACCTCTAAAACACCAAAATAAATACTGTTAATTTTTTGGTTGTTGTTAAATATAGCACTTCCAGAAGAACCACCAGGGAGATTGGTATCATCAAGCATTAAACCATATCCATACTGTTGGTACTCTTTGTCATGATATTTAAGTTTAAAGTTTTTGAAGTTTTGAAATGTAACAATCCCTTTTTCGTGTTTAATAACTTTATCAACATGACTGTCTTGGGTTTTTTTGGAAAATATTTCACTACCTATTTGACCATCTTTTCTGGCATTTTTTGTAATTACAGGGATCTCTTTAGCAGTTGAAACTAAATATGTGTTTTTACTCCATAACTGACTTTTTTGATTAAAGTTATGAGTTCTAGCAAACTCTTCTTTTTGTTCTAATAAGCGGTTTTGATCATAACCTCTTTCAAGTACAGGATAACCCAAAAGATAGTGGCTATGGTTTCAATGTTGTTCTAAATCTTTGGTTTCAAATATATTAGTGCTATCACCCAAACTTTTATAAGTATTAATTGCTGGCTTAATGAATTCATTGAAGATTTGTCAATCACGAAGATTTTCTAAATTAAGTTCCAGTTCTATAACAGCAAAGTCTGCATAAACAGGCTTAATACTGTTAACACCTTTCAAGAAATTAGTTGCGGTATACGCAGTTTTAGGAATATTTGATGTTTGAATAGAAAGCAGTGAATCATTTACCTTTCTTTGTTCTAATTTTTCTTCTTGATAGAGTTTTGTAATATCTGTTTTGTTAGTGTATTTACCAATTGAAAAATAAACAGTTGATTCATATTCATTAAACTCCCTTTGATCATCTGTTTTGCTGTAAGGTCAATGATCACCGATATTTCTCAACCCATCAGCAACATGCAAATTGGTTGCAATGTAAGCGAAAAAAGAACCTTGCTTTTGGGTTGTGTTTTCATCACCTTTTCAGTCAATTAATCAACCAGTGCCATAAGAGTCATTACTAAAGCTAGTGAAATTTAAAGTAAAAGTTAAATCATATCCTTTTCTGTATATATCACTTTCTTCTGGGATAAAACTTGTATTAAATTTAAAGTTTCTGTTGTAAACCTTCTGTTCTACCTTAGGTTGCTGATTTACTTCTGTTCCTTGATCAGATTGTTCATCTTTAAAAGGAGTAGCGTTGTTATCTGTTGTGCTGTCGTTTTTTTCTTCTATAGGAACAGGAACAGGTGAAACAGCAGAACAAGAAGCTAAAAGTGCTTGTAATGAAATTAGTGAAAAACCTAAACTTTTTAGTTTAAACCACATATCAATCTTTAACATTTTAATACAAAAATATTTCAAAACCTTTAATTAATGAAGTTAAGAAGCACTTGTTAAACTTAGGCTTTAGCTTCAAAAATAATTGAGTTCATCTTTTCACTGATAAATTTAATTATTTTTATTCTGCTTAGTTTTAGATGCAATTAAAAAGAAGAAGCTAATGAAAAAACTAATGAAAGCAAATGCAACCATCAAAAAAGTTAAAAATGAAAATAGAACTACTATTGACTCAGGTAAACTAACTAAATTAAAACCAAAAAAATTAATCCCGTTAGAATTTGCATTGGCTTGATAACTACTATCTAAAGCTAGAAAGCCAACTACAAAGCTACCAATTAGAAGAATTAAACCAAAAATAAATCAAAAAACACTATTCAGTTTCTTTTTCATATCGAATAATCTTATCTATCCGTCTTTGGTGTCTACCACCTTCAAAATTAGCTTTGAGAAAATCATCCACTATTTTAATGTTTTCACTGTCAGTTACAAAACGACTGGAAAGACATAAAACATTGGCATTATCATGTTGTCTAGCTAAAGCTGCTGTTTTCTCACTAACAACCAAAGCCGCTAATACTCCTTTTACTTTATTTGCTGCCATACAAACCCCAACACCAGTACCGCAAATTAGTATCCCAAGATCTTTATCAGAATTTTTCTTGACCTTATCAGCTACCAAAAAAGCAAAGTCAGGATAATCATCATTAGCATCAAAATAATTAGGGCCAAGATCAACTACATTAAACTGCTTAGTCTTTAGGTGTTCACTAATAATTTTTTTAAGAGTTAAACCGGTATGATCAGAAGCAATAAAGATATTAAAACTCATTTAATTCAGTTCCAGATAGTCTTGTTTTGTATTGGTTTTAATTTTAGTTAAAAGATTAATTAATCCTTCTAATGTATCAGGATAGTTTTGTCGATTGTATAGTTTTGTTAACTGTTCTAATGATCTATCGATATGACTACTCTTTTGACGATATTTATTTGCAAAAAGAAAAGCTTGTTTGCACATTAGCCTTAAGTTAACTAAACTAACAACATCACAAATAACTTCAGTCAATTGAATTTGCAATAAATCTATCTTTTCACTAAACAAATTAAAAGTACCTAAATAGTTATTATTAGCTTCAGTGAAGATGTTATCAAGTTCTAATTTCGATTTATTAATTACTTGCAATGTTTTAGCAGGAACCTTTAAATCTTGATCGCTAATTAAATTTTCAATCTGAAAAAATTTAAATCGGAAATCATCTAAAGTATAGACAAAAAAGCGATAGTCATCAAACTTATTAACTGCTAAATTAAGTAAATCAGTTAGTGAATTTTTCAGTTTTTCTAAAGCTTGATTTGTTTGACTCATCAAGTAAAACAGTTTTTCATAATTGATTTGATTCTGAGCTAAACTTTGATGGTTTAAGTTATTAATATTTTTAGTAATTAGATCGCAGTTCTCATTAAGCTCAATAATTAAATCATGCACATCTGTTTCATTTTGGAACACTAATTCAATGGCTTTAATTGTTGTTTTTAAAAGCTCATTTTTCTTTTCATATTGTTGAATTTGATTACGAAAATCATCAATATTTTCAACCACTAAATTAATGCTTTTTTCTTTGGAACTAACTGCTTGAAAAAGTCAACTAATCGCCTCTTGAACTTCATCCATATTCCTTTCGCATAGCATTAAATTTAAGTTATTAAGAAAATTGGTACAATCCTTTAATTTAATGGTAATGTTATTAATCAATTCATGAAGTTTTAAGAGATCTTGACTGTTAATTACTTTATTCTTCATTAATTCCATTTGTTTTACCTTTTGAATTAAAACCTTAAAGTGATGGGTTAAATAACTTAACTTAATATCAAACATATATTGCCTGTTAGCAGTAATAATTGTTTGACTGAGAATGTTATGTAATTTGTTTAAAAAAGCAATTTGCTTTTCTACATCTTTAGCTTGAATACAATTTTCTATCTCTTTAAACAAGAGCGAAATCCTGTTAAGAACATTTAAATAGAAAAGCGAATCATATTTTTCAATTAAAAAATTATTAATAAATTGGTTTAAAAAACAGAAAGCCTGACGATAAAAAACTAAACAATCATAGCTATTGTTGAAATAAACAATTGCGTTCTTAGTTAGATTAATGTATTCTTGTTGTTTTGTATATAGATGTTGAAGATTTTTATTTAAGAGCTTAATATTTCTATTAAATCAGATGAAACGATAAATATTATTTGGTTTACTGTAAACTGTTAGTTGCTCAAAAGCTGAATTGATTTCATTATCAAATTTGATTTGAAACTGTTTTAAAACTTTAATGGATTGTTCTAACGCTTGTTTTTCATTTCTTAGTAATTGAAATGGACTAATCACACAATTGAAATTGCTTAATTTCAAGTTATTATAGAGTTGTTTGGTTTTTTTTAAAAGTAAGTTATTTTGTCTAACAAAAAGACAAACTAATAGATAAGCACCAAAAATTACTAGTAGTAAGAAAGGGAGCAAGATTAGAAAAACCAGCATTTTATTTTTTCTTACTTGAAAGTTTTAATAGTTCAAACATTAAATTTTGTTCAATTTTAACGTTTAGCTTAGCATCCTTGATATGGTGAATTTTTCTTTCAAGTTCTGTTTTTCTTTTTAAGGTTTCATTAATATCAACTTCATCAGCAAAAACAAAATTTTCTGTGAAAATCTTAGCTTCTGTTTTTTTAATAAGCATTAAACCAGCACCAATAATTGCTTCTCTTTTTGTTTGATCAGCAAAAATAATTTTGCACAGATGGGATTGAATAGCAGCAATCAAAGGAGCGCGATTAAAATTTAATCCTATGTAACCTTCAGTAGTTTTAACTTGCGCACTAATAATGGTTTTATCTAGTTTTATGCCACTAGGACTAAGTACCAAAAAGCGCAATAACTTCATAAAACTATTTAGTTTTATCAAACTCTTGATTTAATCTTTTTGCTTTTTCAACAGCCTCTTGAATGGTTCCAACATAAAAGAATGCTTGTTCAGGCAAATGATCATGCTTACCTTCCAAAATTTCTTTAAAAGATTGAACAGTATCATTTAAAGATACATATTTACCTGCAATACCTGAAAACTTTTCAGCAACAAAAAAAGGTTGGGATAAAAAGTTACGTATCCTTCTTGCTCTTTCAACAATAATCTTATCTTCATCAGACAATTCATCAATCCCTAGTATAGCAATGATATCTTGTAATTCTGCAAAACGCTGCAAGATATTTTGCACTCCTAAAGCGACTTTATAATGGTTGATACCAACAACACTAGGATCTAATAAACGACTTGTTGATTCTAAAGGATTAATTGCTGGAAAAATACCTAGTGCTGCAATATTACGATCCAACACTGTTTTAGCATCAAGATGGGTAAATGTTGTTGCAGGTGCTGGGTCTGTTAGATCATCTGCTGGAACATAGATAGCTTGAACAGATGTAATAGAACCTGTTTTGGTAGAAGCAATTCTTTCTTGTAACTTACCCATTTCAATAGCTAAAGTTGGTTGATAGCCAACAGCAGATGGCATTCTACCAAGTAATGCTGAAACCTCACTACCTGCTTGAGTAAAACGAAAAATATTGTCAATGAATAACAGCACATTCTGATTATCATGATCACGAAAATATTCTGCCATTGTTAAAGCAGTTAAAGCAACTCTCATTCTAGCTCCTGGTGGTTCATTCATTTGGCCAAAAACTAAAACTGTTTTATCAATCACCCCACCTTGAATCATTTCATAGTAAAGATCATTACCTTCTCTTGTTCTTTCACCAACTCCAGCAAATACACTTAAACCAGAATGTTCTTTGGCAATGTTATGAATTAATTCTTGCACCAAAACAGTTTTCCCAACACCAGCTCCTCCAAATAAACCAATTTTACCCCCCCTAACGTAAGGAGTTAAAAGATCAATTACTTTTATTCCTGTTTCAAAAATATCAACAGTATTTGGTTGTTCATCAAAAGCAGGTGGGTTACGATGGATTGATAGCTTTGGTTTTTTTGGTAATGGTTCTTTTCCATCAATGGGTTCACCTAAAACATTGAACATCCTTCCCAATACATTTTTGCCAACAGGTACCTCTATTGGATGGTTATAGTTAGTAACTTTAACATTACGTGCTAATCCTTCTGTAGGACCTAATGCAATGCAACGAACAATGTCATCACCTATTAATTGGGTTGCTTCTAAAAAAAGCTCACTTTTTTTTAGTTGTACACTCAAACAATCATAAACTCTAGGTAATTGTTTACTTTCTGAAAAGATAACATCAACTACAGGACCAATGACTTGGTGAACTTTACCATATGTTAGGTTTTCTTTTTTTATCAATTTAATTCATACCTCCTATAACTTCAATAATCTCTTCTGTAATAGAGTTTTGTCTTAGCTTGTTAAATTGTAAAGTGTATTTATCAAGTAAATCTTTAGCATTCTTTGTAGCAGCTTCCATTGCATTTTGTCTAGAAGCTGATTCACATAACTTAGTTTCAACAAGGCCACCATACAGAGCCACATCAAAAAACTGTGGAGTAATTAAATTAATTATCGTGGCTTGATCTGGCTCAAAATCAAGTTGTTGATCAGTTACAACCGGATTTAAAGTTTTAAAAGTTTCAACATCGAAAGGAAAAACTTGAAAGAGCTGAGATTGTTGGATTAATGAGTTTTTAAATTTAGTGTAAACCATACAAATTCGATCAAGTTTAAACTCTTTAAATGCATCCATAATCTGATCAAATATCGTTTGACAATAATCAAAATTAATATCACGATCTTGGATATCAATAAATCCAACAGCAGGATTATATTGACTATTTTTATTTCAAAATGATTGGTTTTTTCTACCTAAAAAAAAGATTTTATCATCAGCTTTAAAATTAGCTTTTAATAACTTATTCATGTTCGAATTATGCTGTCCACAAAGTCCTAAAGAAGAACTCATCATTATCCATAAAGTTTTTTGGTTATCTGGTTTCTTTTTTGGTTCTTTTAAAGAAACAACTACTTGGCCAACAGCTTTATAAAATTCATTAAAAAACAAACTAATTTCTTGAAACTGTTTTTTGAACTTAATAAACTTAGCGCGTGACACCATTTTCATTGCATTAGTTATCTTAATGGTGGATTTTACTGTATTCATTCTGCGCTTAATTTCTTGTATAAAAGCCATTAATTAAGTTCCTCAATACTACCAAATTTGGTAATATCATAATCTGTTAATGTACTGATAAAACGTTTCACAAGCATTTTAAAAGCGGTTTTAGTTTTACTTTCTAAATCCTCAGTAAATTCTTTTTTGTTAGATAACTCTTTAAACAGAGGATGGGAACCATTAAATTCTTCTGTTATCCTTTGTTTAAATTTAGCAATTTCATCAACTGGAATAAACTTAATGAAAGCTTTGTTAATAGCAAATAAAAATAATGCTTCATGGACTTGAGAGTAAGGTTTACCATTTGGTTGTTTAATCATTTCCATTACTCTTTTACCATGCTCTAAAACCTTTTTTGTATTTTCATCAAGATCACTACCAAATTGACTAAAACTATCAAGTTCACTATACTGAGCAAGTTCTAGTTTTAAACTGCCAGTTTGCTGTTTAATCGCTTTTGTTTGTGCTGCACTACCAACCCTTGAAACTGATAAACCAATTTGAATTGCAGGGCGTTGTCCAGCGTTAAATAGACTACTAACCATAAACAGTTGGCCATCAGTAATTGAAATAACATTTGTAGGAATATATGCAGAGATATCACCAGCTTGAGTTTCTATAATTGGTAAAGCTGTAATTGAGCCACCACCATTTTCATCATTTAACTTGCATGCACGTTCCAAAAGTCTTGAATGTAAATAAAAAACATCTCCTGGAAAAGCTTCTCTACCAGGTGGTCTTTTTAACAAGAGTGAAAGAGTTCTGTAAGCAACAGCATGCTTAGAAAGGTCATCAAATACAATCAAAACATCCTTTCCTTTTTTAAGTCAATATTCAGCAATAGTTATTCCTGTAAAAGGACTTAAATAAACCATGGAATCAGAGTCACTAGCTGTAGCACAAACCACTGTAGTGTATTTCATTGAATCATTAACTTCAAGTTGGTGTACAATTTGTGCTACTGATGAATTTTTTTGACCAATTGCTACATAAACACAATAAACATCTTTATCTTTTTGATTAATGATAGTGTCAATCGCAATAGCAGTTTTACCTGTTTGTCTATCACCAACAATTAATTCACGTTGTCCTTTACCTATAGGAAATAAAGCATCAATTGTTAAGATTCCAGTTTCAAGTGGTTGGTTAACACTTTTCCTTTTCATTACACCTGGAGCAATTTTTTCTATTTGATCATATTCAGTAGCTTTAATATCACCTCTACCATCAATTGCTTCACCAAGTGCATTGACGATTCTACCAAGCATAACATCACCAACAGGAGTTTTCATTACACTGTGGGTTCTTTTAGCGGTACTGCCTTCTCGTAACGAAGAATAGTCACCAAAAAGTGCTATTCCGACTGTATTTTGTTCAAGGTTTAATACTATTCCTTGAATATTATTTTCAAATTGAATTAACTCATTTAATAAAGCATTTTCAAGTCCACTAACCTTGGCAATTCCATCAGCAACACTAATGACTTGACCAATTTCACTGTTAAATATTTTTTTGGAATACTTTTTAATTTCAGTTTTGATTAATGCTACGTATTCATTTAGTTTATCTGCCATAACTTCTCAATTAACTTAAATAAAATGGGCCATTATGCGTTTTAATTCATCACGCACATTTTTTTCAAATAAATGGGAACTTGATTCTATCCTAATTCCTGAAATTAAACTGCGATCAATCTCAGTTTTATAAACAACCTTTGTTTTAAAACGTTTTTCCATTATTGCAATAATTTTGTTAAGTTGTTTTGAGCTTAATTCAAAAGCAGTAATTATTTTGATAAATTGAATGTGTTTTTGACTTTCAACATTATCAAAAAAATACTTAATTGCCTGTTCAACTAAAGTAAAGTAACCCTTTAAAATAATTACTTTTAAAAAATCAACAAAAACTTGACAAAAATGATTTTTAAACAACTTATCAACAAGTCTTATTTTATCTGGTTTTGTTAGTGTATAAGAATTAAGTAAGAACGATAATGAACCATCTTTAAAATTACGCATTAATTTCAGAAAAAAATGGCACTCTTCATAAATTTTCTTTACTTGTTTTTGCTCTTCACTTAATTGAAAAAGTGCAGTTCCAAATGCTTGTGCATTAATCATCTTCTGTTTCGTTAGCTTCTAAATCTCTAATAAACTTATCAATAAATTCTCTATCTGATTTTTGATCTATTTTCTTGAGAATTAGTTCTTGTGCAGCCAACATAGCCAATTCCACACTCTCTTTAATAGATTGTTCTTTAAGAGAACGTCTTTCTTTTTCAATTTCCTGACGAGCTTGAAAAATCATTAAGTTAGCTTGGCGATTTGCTGTTTTTTCTATTTCACTTTTTAATTGCAAAGCTTCATAGTTAGCTTGATCAACAATTTCTTTAGAAACTATTAGTGCTTTTTCATGCCTTTGATTAGATTCTTCAAGTAGATTTCTTGCTTGTTTTTCTAATTCATTAGCTTGTTTGATTTGTGCTTCTAGTAAATTTTTACGGTTATTTAAAAACCTTTGAGTTGGTTTTCAAAACAAGAAAATCATCAGTGTTAGTAAGATGAAAAATGCTAGTAAATGAGTAATAAATACCCAAAAGTTAGGAAAAAGTTCATTTATTACTGAACTACTCTTAATTTCTCTAACATTCTCAGTACAAGAAACCAAAAATAAGCTGAGTGTAAAAAAGCTAAAAACTAATAAGCTTCATTTAAAGACAAGTTTTTTTGCCTTTACCATCCTTAAGCTCCTGCTACAAAAATTAAGATAAAGGAAATTAAAAGTCCATAAATTGCTGTAGATTCAGAAACAGCAGAACCAATGAAAATTAGTTTAAAAACCTGTTTTTCAACTTCAGGATTTCTTGCTATTGCCTCAACAGCTTTACCAAAAATATAACCTTGTCCAATCCCTACAGTTGAACCTGCAATCATAGTAACACCAGCACCTATATAAGCACCTAGCTTAGCACTAGCGTTAACATCCTGGGTAGTTTGAGTTTGTTGTAATATAACACCAACTGTAGCTAAAATTTCATTAACATGTTCCATAACTAAATATTTTCTTGAATTACCTTTATGCCTTTAAAGTGTAATTCACTTGCATTATTATTTTCCAAACCTTGATTGCGCATTTTAGCCCAATAATTATAAGTCAACATTACAAAAACATAACCCTGCAATACACCTGCAATTACATCAAAATAGATGTGTAATACTGGGGTTATTAAACCTGCAAAAACTGTTCCTAAGCTAAGTGCTAATGGTTGGTTATTAATACTTGAAAAAATAAAAATTCAAAAGTTATAAAAAAGCGCCAAAATAACTGTGCCCGCTAATATGTTTCCCCATAACCTTAATGAAATAGAAAAAAGCGGTGCAAATCCACTCAATATACTAAAAGGATTTGGAATGAAAGTAGAATACTTTTTTCCTTTAACAGTAATTCCAAAGGCAAACTCTTTAAAAAAATTTCATCTTTGGTATCTAATCCCCATAACAACAATCCCAATAAAAGTTGCAAGTCCTAAAGAAAAAGTAAATGTTAAAGATGATGTTGGTGGTGAAATACCACCAAGCAAGCTAACTAAGTTGCTTGATACTATGTACAGAAGCAACATTAAAAAGTAGGGAGCAAATTTCTTATTTTCCTCTCCTAAAAGATCTGCTGTTGTATCTTGTACCCAAACAAACAACATTTGAAATAAAAGCAAAAAATATGAATTATTTTTTAAAGAATCTGCTTTTTTTAACTTAAGCTTATAAAAAATAAAAAAAGTTAGAAGCAGTACAAAAACAATAAAAATACCAAGAATTTGATCAGTAGGGGCAAATGGTTTTCAACCGCTAATTGGTGAGATATCAAAAATACTTTGATTGGAAATGAAATCTATTTGATTAGTTTCTTTTAAAACTATCTCCCGTGGCGACATGTTTTCTTTTCGATTTAGATATTAAATTGATAAAAAAATATTCTAAAGGTAACCAAACAAATGATAACACTAAATTTAGTATTGGAAAAAACAATGTTGAAATTATTGTTGCAATAGTGTTAAAAATTGACGGAGTGACAACAAAAGAAATAATCACAGGAATTAAATAAATTAAATATCTAGCTGTGAAAAAAAATGCAAAGAATGAAACTGCTTTTTGCTTTTTGACATTTTTCAAACTTGCAAAGAAAGATGCTAATCTTAAAACTGATAGTGAATTTAAAACAGCAAAAGGTAATGGCAACATTCAACCAAATAAAATGTCCAAATTATTGCCTTTTGTTAAAACAACAACTGCTATTAAAATTAGTACAAATAACCAAGCTACAAAAATAATTAAGGTAATAATTTTTGCAACCTTTCTATTGTTTCAATTGAACAAAACTTTTAGATGTTAAAGTTTTTAGGTTTAATGTTTGTAAAGGTATTTCAACCTAAGTATTTTCCTTTATCACCAAGTTCAATTTCTATGTACAACAAACGATTGTATTTAGCTATTCTTTCTGAGCGTGACATTGAACCGGTTTTAATTTGACCAGTTTGGGCAGCAACTGCCAAATCAGCAATAGTTGTATCTTCTGTTTCACCACTGCGATGTGAAATTACTTGACTCCAGTTAGCTTTTTTTGCAACTTCAATTGTTTGAATCGTTTCACTAATAGAACCAATTTGATTTAATTTAATCAATATCGAGTTTGTTGTATTTTGTGCAACACCTTTTTTAGCAAGTTCTGCATTAGTACAGTAAGTGTCATCACCAACAATTTGAATATGGCTACCTATGGTTTTAGTTAATTGGTTCATCCCTTCTCAATCATTTTCACTCAAACCATCTTCTATTGAAATAATTGGATATTTTTTTGTTAATTTTTCTAAGTAAGCAATCATTTCTTTGCTTGTTAAACTCCAATCCTTTGCATTAAGGATATTAGCTTTTATTCCTTTCTTGAAAACATAAAGTTTTTTATCTTCGTCATAAAACTCACTAGCAGCAACATCAATGGCAATAGCAATATCATCTCAAGGCTTATATCCAGCTAATTTAATGGCTTCAACCATGATGTCAAGTGCATCTTCTGCAAGTTTTAAGTTAGGCGCAAATCCACCTTCATCTCCTTTATTTGTGTTTAATCCACGCTTTTTTAAAAGATTTTGTAAAGCATGAAAAGTTTCACTAGCCATTTTTAAAGCTTCATGCATCTTTTTAGCACCTAAAGGCATGATCATGAACTCTTGAAAATCAATATAGTTATCAGCATGAGCACCACCATTAATTACATTTAACATTGGCACAGGTAAAACAAAATTTGTTGTATTTAATCCAATTAATTTATTTGAAATGTATTGAAATAATGAGCTGTTTTGTGCTTTTGCTGCTGCTTTTGATACTGCAAGTGAAACAGATAATATAGCATTTGCTCCTAATTTTGCTTTGTTGGGAGTATTGTCTAGTTTAATCATTGCTTGATCCACTGTTAATTGATCAAATGCATTTAAGCCAATAAGCTTAGGGGCAATAACTTTATTAACGTTATCAACGGCTTCGTTAACGCCTTTACCAAAATAATTTTTTGGATCATTATCACGTAATTCAATTGCTTCTTTCTCACCTGTAGAAGCACCTGATGGAACCATCGCTTCACCTACATGACCAGATGCCAATTTAACAACACAAGCTACTGTTGGAACACCCCGAGAATCAAAAACTTGATAAGCAAAAATATCGGTTATTTTTGAATTGATGTTTAGATTTGAACTTCCCATATTAAATAGAAAAATATTGTTAATAAAATTATTATATGTTTTAAGATTTCTATTAATTCAAGTAATATGAAAGAAATTTATTTTGGTGGTGGTTGTTTTTGAGGAATAGAAAAATATTTTCAACTTATTAAGGGTGTTAAAAAAACATCTGTTGGTTATCTCAACTCTAGGATTAGAAATCCTAGTTATGAGCAGGTTTGTTCTGGTTATACTAATGCTGTTGAAGCTGTAAAAGTTGAATACGAAGAAAAAGAAATTTCTCTTTCAGAATTAATTGAAGCACTTTTTGAAGTTATTGATCCAACTATAAGAAATAGACAAGGTAATGATATTGGAACACAATATCGTACTGGTATTTATTGAACTGATAGCAGTGATGAAAAAATAATTAATGATAAGTTCTTAAAACTTCAAAAAAACTACAGTAAACCAATTGTTACAGAAAATAAAAAAGTAGAAAATTATTATCTTGCTGAAGAATACCATCAGGATTATTTAAAAAAGAATCCAAACGGTTATTGCCACATCAAATTTGACTAATTAAATATTTCAGGATTTTTAATAAATAAAAAGGATTTAATGATGTTATAGCAATAATCATTAATCCGTTCAATATGTTTAACAGAAGCTACAAGATCAATTAAGAAAATCCTTTCAGTAACCTCATTTTTATTAATGGTTTCATAGTATTTTGTTAATACAAGGCGATATTGGTGCTCAAATTCAATAATAGTTTTGGTTGCATTTTCAAATTGTAATGTAAGTGGCTTTTCTTTATCTTGTAAACTTTCAAAGGTTTTCTTAAGGTTATTGAGCGCACTTTGATGCAAAGTAACTAGATTATTAAAAATCTTTATATCAAGATGCAAATAATGTTCTATAAACTTAGTTAAATTATTAGCATAATCACAAATGCGTTCCAAGTCACGACTTGACATAATTATTGTGATTGTTAGGCGTAAATGGCTAGCTAATGGAGAATTTTTAGAGATTGTCCAAATTGATTCATTAATTAATTTAAATTCAGACCTATTAGATTGGTCCTCCATTTCATAAATAGTTTTAATCAGTTCTTTGCGTTTTTCTAAATTATCTTCACAAAGTAATTGATTTAATGTTTCATGTGCATTGATTACGTGCTTAAAATACTCAAAAAAAAGCTTTAATAATTTCTTTTCTGAACGCTTTAAAATTTGGTAATTAATGTTTTCCATAGCTAATTTTTCCCACTTATATAACTATTTGTTGCTTTTTGCTTAGGCTTTGTAAATATCTGTTTTGTAGTGCCTTGTTCAATTACTCTTCCATCAGCAAAAAAAATCGTTTCATCAGTTATTCTAATTGTTTGAGCCATAGAGTGAGTAACAATAACAATTGTGAATTTTTCTTTTAGTTGTTGAATTAGAAGTTCAATAGAGTTTGTGGCAATTGAGTCTAAAGCACTGGTAGGTTCATCCATCAAAAGAACATCTGGTTGTAAAGCAATAGCACGCGCAATACACAAGCGTTGTTGTTGTCCACCAGAAAGGGTGTTTGCATTCCTATGTAAATTATCTTTCACTTCATCTCACAATGCTGCTGATATCAATGCCTGTCTTACTATTTCATTGATAGCATTTTTATTGTGAATACCATGTGCTCTTATGCCATAAGCAATGTTTTCAAAAATAGAAAAATTAAAAGGAGTTAATTTTTGAAAAACCATGCCAACACTAGTGCGCAATGTTAAATCATTAATAATTCCTGAATTGATATTCTTACCAAGAAAATATATGTCACCTGTTCAACGTGTGTTTTCATTTAAATCATTTAATTTATTTAAGCACCTAATAAAGGTGGATTTACCGCATCCTGATTTACCTATTAAAGCAGTTATTTTATTGCGTTTAATGTCAAGATTGATATCAAATAATACTTGTTTGTTTTTGTTATATCAGAAGTTGAAATTTCGAATTTCAAATACATTTTTTTCATCAAAATCCTTTTTAATGACATTATCTTTGGTGTTTTTAATCTTTATTAGACGGTTTAAAATTTCAATTTCATTCTTAATACGGGAGATTTCATTTGCATCTGTTTCATTATTTAAACCAGTAATTTTCTTTTTATATTCTTTGATTTGTTGTTCATAAAGCTTTCGGTAATGCTTAATTTTTTCAAGCTTCAATTGAAAATTTTTTCAAAGTGCTTTTATGTTCTTTTCCATAATTTAAAGGACTGAAATTTACTTTTAATTACTAGCCATTTTTGTTTATTTAACAAAAATAAACTCGGGATTAAATAAGAACTGAAAAAGATTAAAAGAATTAAGAAAACAACAGAGACCAATGATGTTTCTAACATAACACTTATTGCATTGCTATTAATAGAAAATAACTGTCCATATATCCTTGTTGTTAGTGTTTGACCTGGCAATGACAAATGAAATAAATTACTAGATGATAACCCTGAAGTGATAAAGAAGGGTGCAGTTTCAGCAATAATTCTGTTGATTGACAAAATTAATGCAACTATTAATCCTTTCAAAGCACTAGGTAAAACTATTTTGAAAATAACTTCACGTTTACTTATACCTAAAGCAAAAGCACTAATTCTTAAATCCCAACTGACATTATTTAGTGCTTGTTGACAAGTTCTTATAAGGAAGAGTAATATAACAACACTAATAGTTAGAATGCCTGCTATTAAACTAGTACCATTAGCTCCTCCAGCACTTAACTGCAAGACTCTTAAAAAGAAAGAAAGTCCAAATAATCCATAAATAATAGATGGCATTGAACTTAGTGAATCAATTACAAAGTTAAAAACATTTTTAACCACTTTTGAATTATTGTACTCGTTAAGTCAAATTGCAATTAAAAGTGCTAGTGGAAAAGTAATGGTGATAGTAATCAAAATAATTACTAGAGTATTTACTAGTGCTCTGCCAGTTGAATCAGCTTCAAATGAAAAAACAGTAGAACCATTATTATTAATAGCAACACTTCCATTAATAAATACAAACAGCAAAATTGCTAACACAAATCCAAAAGCTAAAAAAGTACAAATTAATTCCTGGAATATTTTTAAAAAATAATTAAGTTTTATTCAAACAACACTTTGAAGCCTTTCTTTAAAAAAAAGTGAACGTTCATTGATGTTGTTTACTTTTATTTTTTTAACACTTTGTCTTGTTGATGTTAAATCAACAAAAAGTGCACTAATATTATTGGGAATGAATCACACAACTTGATAAATAAAATTACTAATCTTTTTTAAAAAGGGATAGCGTTCCAGTGTTTTTGGATTAGCTGATCAAATGGCAAAGAAATTTAACAATGAAACTAATATCAAAATTATGATTCCAAAGATATATAAAACACCATTAATCTGTTCATCTCCATTTTCTGAAAAAATAAAAGTGGAAATAACTGATCCCAGTGTTTTTAAATCAGAAGTAAAAAAACGATTGTTGTTAATTACCTCTTGATAGTTAACACTCTGCAAAACAAAGTTAACAGCCATTGTTTCACTAATTGCTCTCGCAAAGGCTAAGGTCAAAATAACTGTTAATTGTGGTTTAATTTCTTTTTTAATAATTTTGTAGATCGCACTTGTTTTATTTTCCCCTAAGGAAACAACAACACTAATTAGATCGTTATTTACATAAGTTAATGTATTTGTTGTTAATGAAATAACAATAGGAATGATCATAAAAGAAAGCATAGCTATCACATTTAAAAGTGAAAGCGGCGGTAATTTCAAGATATCCCGAAAGAAAATGCTTAATATTTGTGATGCAAATAATCCAAAAATTACAGATGGTATTCCTGAAAGGATATCAATAATAAGTGAAAGTTTTTTTCTTATTTTTGGTTTGCATCGATAAACAAGGAAAAATGAAGTTCTAACCCCTATATAACTAGCAATAATTAAAGCTCCTATTGATACAATAAAACTTACCAATAAGGGGAATCAAATTCCTGCCTGTTTATTACCTAAATTAAATTCAAGATTAAACAAGGACTTTGCAAAATCAGGTCCAATTTTTGTTGCTTCTGTTAAAAGAAAAACAAAAAAACTTATAAATAAAACTAAAAACAAGAATGCTAAAGTCTTTGAAAAGATTCTTAAAAGATTCTCTTTTTTTAAACGTTTTTTAATCTTCTTTTGCACTAAAAAGCTCCAAAGATAGGATTACTAAAAGCAAAGTCATCAACTCAAAAATTTTCTAAATTCATTGGTGATGAGTCATTTGTTTTAAACATCTTCTTTTTCTCATCTTCAGTTAATTGCAAAATACCAAATTCATCATAAATATTTTTAATTACATCTTTTTTTCCATTAAAAAATAATCAATTAAAAAAAGTTTTAATTTCATCAATATTCTTATTCTCTTCAGAAAGTGAAACTATTGAATTTAAAGGACGTACTCATTTATACTCATTTTGGGAAACAGTTTGATTAGAAGGCAAAACTTCCTTATTCTCATATTTAATTGGTAGTATCTCAAAACCATTATTTTTAATAGCTTGAAGGTTGTTTTGAATAAATCCTAAACTAAGGTAAATCATCCCGAAAAGATTAGGATCTTGTAAGTTAGTTACAAAAGTATTAAAAGCTTCAATATTTGTTTCACTAGTAGGTTTAGCAAGAGGTCCATAATTAATAATTCCTTTCAAAATATCTCTAGTTTGAGAATCTAAAGTTTTATCTGATTTTAAGGCGGAAAAATTTAAAAAAGCTTCTGCTGTTCCTGAAGCAAATGACCCACCAGTTCTTGGAAAACTTGTTAAAGGGTAATCTTTTTCATTTGACATATTTTTTGTATCCTTTTTTACAAACTTATCAATGTTAATTGTTTTAACACCAGCAAATAAGTCGTAGAGATCATTTATATTATCTTTTGTTAAAAGTAACTTACCTTTTAACTCCTGTGGTGCCTTATAAATTACTGCAATAGCATCTTTGCCAATAGTTAAAGTTTTCAGCTTTTTATCTCTTCATTTTTTTTCATTCTCTTTTGCATATTCCTTAGGGTTTTTAGAGACATTTCCGATATCAGCAAAGCCGTTAATGATTGCTCTGATTCCAGCATTTGATCCGCCTGCTTGCACACTAATTTCAACAAGCTTATCATTGTCATTATTTTCATTTAGAACATAATAAGAACTTAATTTATTTAGTAAAGGTTGAACTGAAGAAGAACCAACAGCACTAATCAGGTTTATGTTAGCACAACCACTAAGAAAAAAAGCAGATGCTAATGTTAGTAGCGTCAATTTAAAGAAATTTCTAAACTTTAGCACTTTTTAAACAGATTAAATCTTTTCTAAAGAGAATTCGAATTGACACTCAGGTTATTATTTAGTCTAGTTTTTTCACAAGCCAATTAATTTAAATGAAATGCATTTTTATTAATTGCAAATTTAATTTCTTTTCTTAACAATTTTCTTTTTTCTTCATTTTAAAAACAAAACAGCAGAACTTATCATTAAGCTAATGATAATTCCAAAAATGACTCATAATATTAACTCAACATTTGTATCTTGTTTTTTAATTTCATTTTTTTCTAACTTATCAACTTCATAGGATTTATCTTGAGATTTAAGATTAAAATCTGGAACTCTCTTGAATTGCAGGTTATTTTGTTTTAGATAAAATGATAATGCTTTGAAAAGTTGTGTTTGATTTTCACTAGTAATATCAAATCAAATAGAATTATCAAAACCATTGGCATCTACAATTAAAAGTGTTTGATTATTTGTTATTTTTAAATCATAGCTAATACTAAATACTTGGTTATCATTATTAACACCTTTAATAAATAATTTTTTTGATCTAACATCGACATCAAAAACTAAATTAAATTGATTTTTATTTTCTACTAAATGGATGTTAATAAATTTATTCTTTAGGTTAAAGCGGACGTTTTTAGTTGTTTGCAATCTTTTTTCACTTAGAGTAATATTGAGTTTATCAACTAGTAATTGTTCGTCATTTAAACTAAAAGGTTCATGAAAATCAAGTTGATAATCCTCTAGAAATTTAAATGAAGATGACTTTAAATCTAATTCAGCAATTTTTAAGTTATCAACTGATAAAAATACCTTGTCTTTTTCTAACTTAAATCCCAGGTCATTTTTATCAACAAAAAGTTTGTTAGTATGAAATTTAAATAAATATGTTCTTAATTTAGGGTTTTTTTGATTTTGTGATAGAAAATACATTTTGTCTTTAATAATTTCAGAATCACTTTCAGAACCATATTTGAATAGTTCATTGCTATTATTTATTTCTGCTAAAACATAAAATATACCTTTCTTTTCATTAGTAGGCTCTAGATGTTTGAAATATTTTTTAGGGTTATCCACAAATGTTAAAGAGTTACTGTTGATATCTAAATCAATATTCTTATCCTTAGATTTAAAACTATTTTTGATAATGAAAGCCATTTGTCTTTTTTCATAATCATTAAACTTTTCATTACGTTTTTTAAGTTCAGCAACATTAAAGTTTTGAACATTTTTATTTAGTGGAATACGAAAATCTTTAGCAATTCAACGCTGTTCATTAAAATCTTTTCTATATTTAAATTTGTATTCAAAACCATCCTTAGTTCTTTTGATAGTTGAAAGTTTTTTAATACTGTCAAACAGTGGTTCAACACTATAGTTACTAATATTTTCTGATTCATATCCATCTACAAAAAAATTAAGTGCTAAAAATTCAATATTCATTTCATTATCTGCTTTCTGTTTTTCTGTTAAAAACTCCTCAATTGAACTTAAATCAAAATAATTCTTGTTATTTCTTAACAATGTTATTTCATTTACAGAATTACCAATTTTTACTTTAACAGTTAATGTTGATCTATCAAAAATGGTTTTGACCATTCTTGGTAAATTTCTAATAAAGAATAGTTCTTCTTTATTGTTTGGTGACATTTGCAATAGGTTGTATAAATAATCTAAATTTGGATAAAAATTACTTTGATTATTGGTTGAAATATTAAAGCTTTTTAACGCTTCATTTTTATCAATTGATTCATCAATTCTTTGATTAATTAAACTAACTTCTTTAATTGCATTTATGAATTTATCTTTAAATTTAATAGTTGGTTTAATTTTTGTACCTATTGTTTCTTTATTTAGAGTTTTTTCAGTTTGACTAACAAGCGAATTAATATCAAATTCCAACTGTGAAATTCAAAGCTTTGAAAGATTACTGAAACTAATTGGTAAAACTTCATCAGGATTATTTTGCCTTAGATAAAAACTTATTTGATGATGAACAACTTCTTGTTCAGGTAATATAGGTGTATGTTTAAAAATATCCCATTTACTGTTGAATGCTTCACGTCTACTATCTTCAGTTTCTGGTCTAACTACTAATCCATTACCTAGTCTTAATATCGTTTGAGTTATTTCACGATTATTAGTAAATGCTTCAATGATTTTTCTATCCTTTTTCATTTTCTCTTTAAACTCATTTATACTCATCCAATGAGCATCAACTTGTTTGTTGCTGTTAATAGAAAAAAATCAACTGCTTCTACCAGGAAAAGGTTCGTTTCACTGAGCGTTATTCTGTTCAAAAACTGGAGGAACTTTCACTAGTTCTAAATCTCATCAGAAACGATAAGCTCTAATTGGTGTCATTGCTAAACCTCATCAGAAATCTGGAGCTACAAAAATATTATTTTTTCAGTCAGCAAAAAATCCTCTTGATTCTTGGCCAAGATAATATCACATAGGTGCATTAAAATCAATTACATAATTACCATTATTACCTTTTCTTAATCATTTAATATTTCTAATAACATCTTCTGGGGTTGGTTTAATCATTCTTAAAGTCGATCCTCACATGTGAACAAACTTAGCTTCAATAAAATATGCTTTTTTATGACTGGGCTTTGTAAGGTCTCAAAAATAAGGATTATCAGGTTTATATCAACTTTTGTTTCAAGAACCATCTGGATTTGTACCAGTTATTTCTATATTCCTAGAAGGAACTTCGGTTCATTTTTCATTAGTATGACCATCTCTTGGAAAAAAATTAGAAATTGCTTGACTATGAAAATAAAATCTCTGCTCACTATGGATATCAGATGACCATGAGTTTCTAATTCCAGGGTATCTAAATGTAGATGAAAATGCATGTGCAATATAACTACTCTCAATTTTTAACTTTATGTTCTTTAGATAATAAATATTGTTGTATCAGTGAAAGTTATTATCTCTAATAATTTGTTTGTAATTATCACTATATTTATTAACTAATTCACTTCATTCATTCGAATAAGTTTTTGCAAAATAATTATTTTGATTAGGTCTTTGAATTACTTCATTATCAAAATTAAAGCTTTTTCGAAAATAATCGTTTTTTAAATTAAATCCAGCTGTATAGCTTCTGTTTTGAATTGTGTTTGGAGAGAGATAAAGATTAGTATCTACCAAGTTTTTTTCAAGTGAAGCATTAGCTAAAGCAACGGGAAAAAGAAAAGAAACTAATAAAGGAATAAAAAGATATGAACGCATAATTTATCTCATTAAATAAATGTGTATTTAATGAAATATTGACAATTTTTATTGCTAGTTCTTGTTAAAAATTATCTCTTGTTTTATTTTTTATCATCTTTTGTTTTTTATTTCACTTATGAAAGTAAATAAAAACAGTGATAAACAAAACTAAAACACTAAAACCAATAATCAAATATACTATAATATCTGTTTTTTTATCTTCACTATTAGATTGAATAATTTTGTCTATTTGAAAACTATAGCTGTTTTTCTTTAAGCTAAAATTAGGTTCTTTTTTAAATTGAAATTTTTTTAATGAGAGAAATAACTTAAGCTCATTTTTCAGTTTCTGATTATTTTTCTTATCAAGATCAAACCAAATATATTTATTTAAGTAGTTATTTGTTGTTCTAATTTGCAATTCATTTTCATTTTTGATAGCAATATCATAAACAAGGCTAGTTAATCTATTTTTACTTATATTAACTTCAATATTGAGCTGATTTTTAATTGCATCAACATTTAAATAAAAATGAAGCGAACTATTTATCTTTAGAGTAGTTAATTTGATCTCTTTAGGAAAAAGATCTAAGCTAGATTTCTTTTTATCTATTAAAAGATTCATTTCACTTAAAGCCAAATTTAGTTTTTTAATTAATAGTTCTTTATTAGCTAAAGAAAAATAATCATTTATATGAACTTCATAACTTTCAAAAAATGAGGTATTATTTTCTTTTGGATTAAATTCATCTATTTCAACATTATTAACAATTACCCTTAATTTATCACCGCTTGATTTAAATCAAATATCATTAACATTTAAAACTAACTTTTTGGTTCTCATTTGAAAAAGATAAGTTCTTATTGCAGGAAAGTTTTTATTTTGTTCTAAGAAATACATCTTATCTTTAATGATTTCGCTATCATTTTTAGAACCCCATTTAGTTAAATCATTACTGTTAATTATTTGTGCTGAAACATAGAATATACCATTTTTAACACTGGGCTTAATTAAAGATTTTTTAATTAATTTCTCAGGATCTTTTATGTATTTTATTGTAGCGGATGAAATATTTAAATTGTTTTCTTTTTGAGAAGTAAAACTATTTTTAATTGCATATGTAATTTGTTCTCTTTGATAACTATTTAATCCGTTTTTTTCAATGTCATTAACACTGAAATTTTTCTGATTTGTAACAATAGGAATTAAAAAATGATCTTTTAAAATATGATGTTCATTGTATTCAGATCTAAACTTTAAACTATATTGAAAACCATTTTTAGTTTTCTGAATAGAAGGCAAATTTTTAACTGACTTAAAAAAAGAATCAACTGGTTGATTTTTTAATGTTCATCCTGTTTTATTCACAACAATAAAATCAAAAGTTAAAAACTCAATTTCATAAAAATTAATACCAGTTTGATCAGCATTTAAGAAATTTTCAACATCACTTAATAAAAAAACGTTACTGCCATTTTCTATAAAAGTAATTACTTTATTTTTGCCACCTATCTTTATATTTATCGTGATGGTAGTATCTTTAAAAATACTTTTAATTAGGTTGGGTAATATCCGAATAAAATTTAATGTTTTCTCTTGTTTTGGATTAATGTTAATTAAATTACTTAAGAAAAATATTGCATCAATTCTTTCATCTTTTGGTTCAAAATCAAATTTAGATTCTTCCTGTTTTTCTAATAAATCTAAAGCAAATTGAAAAAAAGTTTTATCTTTATTTGCTAATTGATCAAACTCTTGATTTAGCTTACTTACCATTTCAATTGCATTAGAAAATTGATCACTAAATTTCAGCTTTGGTGTTATTTTTGATCCTATTAGTTCTTTGTTTAAAACAGTAGTTGGATTAGCTATTAAAGAATTTGAATCAAATTCAATTTGGTTAACAATGATCTTAGCAAGTTGATTTGTTGCTATAGTTAATTGATTTTGTGATTGAAAATCATCTTTAACTAGATAAAGATTAGTATCAATCAACTTACTTTTTATGGAAGTGTTAGCAAATAATAAGGGAATTATCGCAAAGGAAACTCACTTTAAATAGCGTTTTCATGACATAATAAGCTTTATATTCCATAAATTAAATAAAACTTATTAAGTGACAGTTTTTTTACTAACGCTTAGTAAATTGTGGTGCACGTCTAGCACCATATAAACCAAATTTTTTGCGTTCCTTAGCACGCTTATCACGTGTTGTTAATTTTTTGGTTTTTAATAACTTTTTAAGATCTGGATTAAATTTTATTAAAGCACGAACAATACCTAATCTAATGGCTCCTGCCTGCCCAGTAAATCCTCCACCTTTAACAACAACATTGATATCAAAGTTATCCTTAAGTTTGGTTAACTCTAAGGGTTGTTCCATATCTTGAATCACCAATTTATTTGGAAAATAATCGCTAGGATTACGATGATTAATGGTTATTTTACCCTTATCTTTGCTTTGATATAAATAAACTTTAGCAGTAGAAGATTTACGACGACCAAGTCCATAAAAAGATTTTTTATCCATATCTTTTAACTTCAATTAAGTAATGTTGGTTTTTGTGCTTCCATGTTGTGCTTATCATTCTTAAAAACATGTACTTTAGTTATTAATCTTCTGCTTAAACGATTATCAGGTAACATTCCCTTAACAGCATTGAAAACAAGTTTATCTGAATTTTTGTTTATCATATCCCTTCCACTAGTTTTTTTAATTCCACCCATGTATTGAGAGTGATGATATCAAAACTCATTGTCTTTTTTATTTCCAGTTAAAACCACTTGATCACTGTTAATAATTATTAGATGATCTCCACAATCTTGATTAGGAGTAAAATTAGCTTTATTTTTTCCTCTAATTAAATTTGCAGCTTTAACTGCTAATTTGCCTAAAACCAAACCACTAGCGTCAACAAGATACCACTTCCTGTTTTTAATGGCTTCTTCCTTTGTAAGCATCGATGTTTTTTGCATATATTTAGATACAAAACCGCATTCTATTTTAATATTATTACTTTTCTTCAAAAAGTCCTTTAAAGTATTTATCCACCATTTTTTGTGATAAAGAGCGTTTCTTTTCTTTTAAAAAAGGCTTAGCTAAGTTTTCCAATTGGGTTAAGTTCAACACTTCAGTTTGATACTTTTGATTTTTTGTTTCTTCAGTAATTGTTGTTTTATATTGATGCTCACTAAAAGATGCTAAGAATAAATTTTGGTTAAAAATCTGAAATATAGTTGCTTGTACATTACCTTCTTCTATTGCCTCTTTTCAAAGTCTCATAGCAATTTTTTGATCTTCAAATAAGATAACTATCCCAAATGAAGAAGCTTTTACTATTTTGGTTGCTTTCACTATATCACGAGCACTATATTTGTCTATAAAGTTATTGAAATAACTTAAATTATTCTGTTTATCTTTATCATTAAATTTAGTTTGAAAAACACTTAAAAGATTTTGAAAATGATTAATTCATTTATTGTCATTAAATGCATTAATTGCAAGAGCAATATTGGCAAGATTTAGTTTGCTTGTATCTAAGAATTTGTTATTAGTAACTTCATCATTTTTAGGTGTCTTTTCTGATTTATCTATAAAAGATTTTTCAAATAAAGAAAAGTGCTTTTGGTCTTGTTTGTGATTTGATTTTTTTATTTTTTTCTTTTCCAAATATTCAGTTTCTAAGTGTAGAGATTTTTCATTACTTGGTGAGCTTATTGTTAAAGGTTTTTGATTAAAAAATTCATTATAGCTAATAACAATAGCTTTTAGCCTATCAATTAAAGTTAATTGACTTAAACCAAAATTAGTATTTTCTTCAATGGCCTTAATTAAAAACGCATAATGCTGCTTTTCAAAATTAAGTGTTTCTATCATTGTTTTTTCAGTTGAATCTAAAACATTAATATTTTTTAGTTTTGCATACGCATATAAATTAACTGTTAGCGCAAACAACTCTCTTAAGAAGTAAGTGAAATTTAGACCATTACTTTCAAAATCATCAAGCAAGTTAAATGCTTCTTTTATATCTCCTGATAAAACTGCTTTAATAAAAGTAAATTTTGCATTTCTATCAACGATATTAAATGTTTTTTCAACATCAGTAATACTAATTTTTTCACTATCAGAGAAATTGCTAATTTGATCTAGTAAGCTAAGCCCATCACGCAATGAACCTTGGGATAAATCAGCAATTTTTATTAATGCATCTTTCTCTATCTTAATCTTTTCTTTTTTTGCTATATCATTTAATCTTTCAAGGATTAAATCACTAGTTATTTTTTTAAAAAAGAAGCTTTGACATCTGGACAAAATTGTTAATGGAATCTTGTTAAATTCAGTAGTTGTAAAAATAAAAAGAACATAAGGTGGTGATTCTTCTAAAGTTTTTAACAAGCCACCCCATGATTGGGTGGTTAACATGTGTGCTTCATCTAAAATATAAACCTTTTTTTTAAATGTGAAGGGATGATTGAAAACATTTTCTACCAACTCTCTAATATCATTAATACCATTTTTAGAAGCTGCATCTATCTCAACTATATCAATGGCACTATTAGTGTTAATACTTTTACACACATCACAACTATTACAAACATCAATTTGATCTCAATTTAAGCAGTTTATCGCTTTTGCTATTATCTTTGCAAAAGTAGTTTTACCTGTTCCTCTTTCACCTGAAAAGATATAACCATTAGGTAGTTTATCCCTGTTAATAGCATTCACCAAGATTTTTCTTATCGATTCTTGTCCTAGGGTTTGTTTGAAATTGATTGGCCGATATTTTTGATAAAAAACTTGGTGCATAATTTTTAAATTGAATCTGGTTTTAAAATTTTGGACAAATATTGGGCAGTATAAGATTTATTAACTTGGTTTATAAGTTGTTCAGGTGTTCCTTGAGCAACAATTTGACCACCATTGTCACCACCTTCAGGACCTAAATCAATGATATAGTCAGCAACCTTAATAATATCTAAGTTATGTTCTATAACAACTACTGTATCACCATTCTTAATGATTCTTTGAATTATTGTTAATAGTTTGTTTATATCTTCTAAATGTAAGCCAGTAGAGGGTTCATCTAAAACAAACAAAGTTTTACCAGTAGATTTTTTTTGTAAAAACTTAGATAACTTAATTCTCTGTGCTTCCCCACCTGAAAGAAAAGTGACATTAATACCTAATTGCAAATATTCTAAACCAACATCACATAACAACCTTAGTTTACGTGATATATTTGGGATAGCTTTAAAAAATTCATAAGCTTCTTTACAAGACATTTGTAAAACATCAAAAATTGATTTTCCCAAATATTTAATTTCCAGTGTTTGTGAATTGTACTTCTTGCCATTACATACTTCACATTTGACATAAACATCAGGTAAAAAATGCATTTCAATGCGAATCACACCATCACCAAAACACTTATCACACCTACCACCTGGAACATTAAAAGAAAATCGTGAATTTGTATATCCTCTTGCTTTAGCTTCTTTTGTGTTGGCAAATAAATCACGAATATCATCAAAAACACTAATATAGGTTGCAGGATTAGAACGTGGTGTTCTACCAATTGGGTCTTGAGAGACAACAATTATCTTATCAATGTTGTTAGCACCAATTATTTCCTTATATGTATCTTTTTTAACACCTTTACGATAAAGAATTCTTTCTAAAGCTGGAACTAATGTTTGATTAATTAAAGAGGATTTTCCAGAACCTGAAACCCCTGTTATCAAAACCAATTTATTTAAAGGAATGGTGACATTAATATTTTTCAAATTATTAACTTTAGCACCCTTGATAATAATTGTTTTACCATTACCACTATGTCTATTTTTTGGAATGGAGATTTGTTTTTTACCACTAAGATATTGTCCAGTAATTGAGTTTGAGTTTTCCATTACTTGTAAAGGTGTACCGCAAGCAACTAATTCACCACCTTCATTACCTGCTTTAGGACCAATATCAATTAAATAATCTGCCGCTAACATTGTTTCACTGTCATGCTCAACTACTAATAAGGTGTTACCTAAATCACGCATTACCATCATTGTTTTAATTAAACGCATATTGTCTTTTTGATGCAATCCAATAGAAGGTTCATCCATTACATATAAAACACCAGTAAGTTGAGAACCAATTTGGGTAGCTAATCTAATTCTTTGTGCTTCTCCACCTGACAGCGTAGAAGCTCTTCTTGCAAGATTAAGATAATCTAAACCAACATTTTTAAGAAAAGAAAGACGATTAATAATCTCTTTTAAAGCTAATTCACCGATCTTCTTTTGCTCATCATTTAACTCTAGTTTTAATAGAAAATCAATACTTTTATCAATGGAAAGTTCAGTAAAGCTAATAATGTCAATTCCTCCTAACTTAACACTTAAAGCGTCTTTTATTAATTTTTTTCCATGACACTTTTTACATGTTATTTCAGACATGTATGCAGAATATCATTCTCTACTTACTTGGCTGTTTGTTTCTAGGTGTCTTCTTTTAATTAAATTAGCTATCCCTTCATAATGCTCAAAGCGGATATTCTTAGCACCTGAATTGGAAATTGTTTTTATTTCAATAGGTTCATCACTTCCTTCTAAAATTAAATTAAGTTGTGACTTATCTAACTGTTCAATTGGTTTATCTAATGGAATTTTATAGTGATTAACTAAAGATAAAAAGCGCTGTCAATCCAAAGAAGTTCCATGCACAATATTTTTAAAAATATCAATGGCTCCTTGGTTAATAGAAAGTTTAGAATCAGCAATTATCTTGTCTACATCAGGTTCATAACTAAAACCAAGTCCTTTGCAATATGAACATGAACCTAAAGGGGAGTTAAAGGAAAATAATCTTGGTTCCAATTCACTAATAGAAAAACCACATTTATCACAACCATGATTTTTGCTGAAATTTAATATTGTTCCATCTTCCTTAAGAACTTCTATCTTGCCATTAGTTAACCTATCAATGGTTTCAATGCTATCAACTATCCTTGAATAAGTTTGATTATCTTTATTGATGATAATTCTATCGATCACTACACTAATGTTGTGTTTAGTATTTTTATCAAGTTTAATTTCATCATCTAAGGTGTAAATCTGGCCATCAACTAAGACTCTAAGAAAACCTAATTGCTTGTATTTAATAAATTCATTTGTAAAAATGCCGCGCTGATTTTTAACAGTAGGTGCTAATAATTGCACCTTTGATTTATTAGGTAAATCAAAAATCTGATTAGCAATTTGGTTAATTGTTTGCGTTTGAATAGAACCATGACCATTAGGACAATAAGGGGTCCCAATTCTAGCTCATAAAAGTCTTAGATAATCATAGATCTCAGTTACTGTACCCACAGTTGAACGTGGGTTATGTGAAGTGGTTTTTTGATCAATGGAAATTGCTGGTGATAATCCTTCTATAAGATCAACATCAGGTTTATCACTGTTACCTAAAAATTGGCGTGCATAAGAAGATAGAGACTCTAAATATCTTCTTCTCCCCTCAGCATAAATTGTGTTAAATGCTAAGGAAGATTTACCTGATCCTGATAGACCAGTAATAACAACAAATTGATTTTTAGGGATATCAATGTTAATGTTTTTAAGGTTATTTTCTCTAGCACCTTTAACCCTTATAAAATCATTATTTTTTCATTCCGGTTTCAAAGTCAATGTTTTTAATTGAAAAATTTTTAACTAACTTATTATTAGGATCATTGAAAATAAGCGCAATACCTTGTTTATTTTGATATGAGATCAAAAGTCCACGAATGAAATTATAAGCGTGGTTAGTGAAATCATTGCAGCAGTTTATCATTAGTAACTTAGGATTTAACAAAAGTTTAAATAGCAAGTAAAGCTTTAGACTTTCATTTTTGTTAATTTTGTTTAAATCACTAAATAACATTCAGCTGCTAATACCATTCTTTTTGGTTTGATTTATCAATAAATTAATGTTGTTAATAAAGTTAAGTTTTTTAAGATTTTTTTGTGATCTTAAAAAGAAATTAATTGTTTTTAATTGTGAATTCAAAACATTAAGATAAAGCTTATTTGAAGCTTCTAAACGATTGATATTGCACTCTTCTGAATCAATAAAAAAAGTTCTCTTAATTTTCTTGTTTGTTTTTTTAAGTCTTAAGTGCAATGTTTTGTTTAAAATACAATATCTTTTCATGATGAATTTAATCTTTTGTAACTTTTGATTTGTTTCATCGCGAAAAATTCACTTAACTAAATTAATGTCTTTTAAAAGTAATTTGTATTTTTGTTTGCATTTTTTTAGCTTTTGTTTTAGATCATTCTTGGTTTCTTTATTTGTTGTATCTGTTGTTGAAGAAAAACCGCGTGATGCTAACAGGAAACTAACAGGGGTTTCAAAATTTAGATCTAAATATTTATTAATAACTTTGTCAACTAGAAAATTTCACTTTGGGGAGTTTAAATCATTTTTTAACTTATCTTTTAGCAAACTAAAATGTGGTTCACTACGTCCTTCATTTCAAATAAAATCATTAGAACCACAACTTAAAAACTCATATTTATTAAAGAATTGTTGAAATTTTTTACTTAGGCTATTTCAAAGTGAAATAAGAACAAAATACAAGTTATTTAACTCATATTTTTGTCAGTTAATTTTTTGATAATTAGCTAATAAGCTTTCATAAAAATATTGCTTTATTTGAAATGCAAAGTTAAAAGTCTCCAAACTTTTTCTCATGCTGATTAAACCAATTGCATATTGCACTGTATCAATAAACTGTTTGAAAACTGTTTTTTGGATTAGAACTGAAAAACTGTTATTATGCTTACCTCTATAATGATTTTGATTTAATAAGATGTTTTCTTTTAGTTCATTAACCAATTTTTCATGAACATACAAATTGTTTGATTGTTTAACAAAATTTTTAATAAAAGATAAACGTGCTTTGATCAAAAATTCATGAACCTCTTTTTTCGTTAGAATGAAACGTGCAGACATTATTTTAAGTTCTTGATTAATTGTCTGGTACTTTCAGTTCAAATTAGGACCTGATTTTAAGCTGATAATTTCTTGATAAAGATTTTGAATATTAGCAAAGATTTTATTGACTAAATTAATACTTTCATCAATAGTTTCTACAAGTAATTTTGGAGAAAGTAAATCCATTTTCTTAATTTCTTTCAAAAGTAAAATTCTTGTTTTAATCCGCTTAGATACAATCTTTTTTTCTAAAATTCTTTTTCTATAAAAATTAAAAAAACTTCTTCACTTAATCTTTCAACCACTAAAAATTAAAGAAAAGGTGAATGAATTAATTAATTGCAAAGCATTTTTATGTTGTTTTGAGAATGTTTTCTTAGCATCAAAAAGTCTTTGTTTAGCTCTTTCAATTTGCAATTTTTCAAAATAACTGTTATCTCGTGATTTAGCTTCATAAGCAGCTATTTTCAGTTTGTTTTTTAAGTTATTTTTTTGTTCGTTCAATGCATAGTAACGCTCAAAAAAACTTTTTATTACTTTGTTATTAAAAGTAAATAATTGATTGAAAAACATGCAATTAAAATCAACAAGTTCTCTTTCGTTTTTTTCAATTAAAGTTAAAAAATTATTGTATTCAAAATCAACTTGTTCAAAGTAGATTGAATTAATAAATTTTGATGTAAAAAACTGATTAATGACTTTACTAGTTTTTTGATTAACATTTTCAATTTTCAAAATTGAAAACCTAAATAAATCATTAAGCGCATTAAAACCTAAAAATCACTTTAACTGTTTAGGTATATTCACTAAATTAAAGTAATTTTGATCTTTTTTATAACTTAAATCTTGTTTTAGAAAAATGCTAAATGAATTAGGAAATAACCACTTATTTTTATTTCTTAAATCTATCTCTTCACATAAATTAAACTTAATTTTTTGAAGCGGTAAATATTGATGGTTATTAACAAGCAAAAACCCAGAATAGGGTTGTTTATTTAACTGTAAAACTTGTGAAAGGGTTTTTCATTGTGCTTGATTTAAATTATCAATGAGATAGTTACCATACTTAAATAATGATAGCTTTGCAACCCCAAAATAGTTAGCATCTAAAAAGGAGGTACGAATCATTAATAATGACAAATCAACCCACTGTTCTAGATTATCAAAAAGAGATATTTTCTTTAGCATGTACCTTTCAAATTTTAATTGCTAATCTTTAAGGTACTTAAATAGAAATAATAGTTGAAAGAATTAAGGGTGCTTTTGATGTCATCTTGGTGAATAAATTCATTACTGCTCGTTTAATAGAAACCTTCAATTCTTTTGAAATTTCTTTACCTATTCGTTTTTTAATCGTGGTGAAATCCTTAATATCATTAATTTGTTTTTTAATTAATTCTTCCATTTTTGTTTTTAGTTTATCTTTTTCTGGTACATCTAAAGATACTCCTAAAAAAGAATAAGTTATCTCATTTAAGAATTCACTCTTTTTTTGATCAAAATAAATGATTATGATCACAACTCCAGATTCTGACATTTGATCACGTTCAAACATAATGCTAGCTCCAATTTCTTGTAATCCAGCAGAATCAACACACTTAGGATTTAATTTCAATTCATTTTTTTTGCTATCTAGTTTTTTGTTTTCAATAGTTAAAACTTCACCGTTAAAAAGAATTAAGATTTGATTTTGCTCAGCGCCAGCTTGTTTTAAAACCATTGTGAAGTTTATAAAATCCCTATAAAGTCCACCAGTTGGAATAATATATTTTGGTTTTAAAGAACTAACTAAAAACTTCATATCTTCATCACTTGCTTGGATAGAAAGGATTTCTCTACCTAAGTTGTAATAACTAACTTCATTACGTGCAATATCATCAAGGATTTGTGCTTCTATCTCTTCATAGCCAGCAACTTTAGGAGTCATAAAGATAAAAGTATCACTCTTACGATAACGAATTCTTTCATCTTCGTTCATTCCAATTTTGAATAATTTAGCATACAACTTATCAGGTGGTGAGGTGAGAACAACAATACTGTTGGTTGAATTGTTAATCTCCTCAATAGAAATGGTATTTAAATGCGTGTTATTAAACAATTTTTGACGCACAATTGTGTTAAAGAGATGCACAAATGATTGTGAATAGATAATAAAAGGACGATTTTGCATCCTTGCAATCTGGGCAAGAGTCATAACACTGTAAGCATTTGAATCATAACAAGCTACAAATATTCTGCCTTTGGCTGGAGTTATGATCCTGTTTAACTGTTCTAATGACTTGTGTTTAGGTGTTGTAAAACCACTGTTTCTACCAACTAAACCAACTCCTGTTATTAATAACAATGTATTGTCAGAAAGTTTAGGGATAATTTGATTTAGTTGATTTTCAAAAGCAATGTTCTTATCATTTAAAACAATGAAATCATCAATAAAAACGATGTAACCATTATCAGTATTTAAAGCAAATCCAAATGATGATGGTAAAGAACTTGATACTTTAAAAGGGGTAATAGAATGATTAGAAATTTCGATTGTTTCAAGTGGTTTTAATTCATGAATTTCCAATTTATCACGTGCAATATTAAGTTTGTTTTCATTAATTTTGCTTTTAATTATAGAAGCGCCTATTGAACTTGTGTAAATAGGAAAAAATCCTACTGTATGGAACAAAAATTCTAATGATCCTAAGTTTTCAGTAATGGCATTACCTATAAAAATACCTTTAACTCTTGCTTGATTTTCTTGAATTCAACTAAAATCAGGAATAATTTTTTTAACACCTAAAACTGCTGTTGTTGGTGTTAAGCTACCAACATTAAAGATAAACACATCATTGTCAATTTCAAGCACATAGCAATTTTTACCACGTTCATCTTGACCGCCGAGCGCAAAGAATTTAATCTTAGCCATTTTTAACCCCGGAGGTTTAAATTTTTAATCAAATCACGGTAGGTTTCAATATTACGTTCTTTTAGATATTTAAGTAGCCGTTTTCTTTTTGATACCTTTGTATATAAACCACGCTTAGAAATAAAATCCTTTTTGTTTGCTAACAGGTGGTCTGTTAATTTTTTAATCTGATCTGTTAATATAGAGATTTGTACTTGCACACTGCCAACATCGTTTTTGTGAAGTTGATGAGCCTTAATGATTTGTTCTTTATCAATTTTCATTAATTAATAAACACGTTGAATTATAACAAAATTAGAAAACGGCTCATTGCCAAGGTTAGAAGTAATAAATTCTCCTACTTACTTATTACTTATAATAAATATAGTTTTTATTTTTATTAATAAATTCTCTCGATATTTTCGATATTTAAATCTATACATCTAAACAAATTAACAAAGCCATTTAACTTATGGACTCAACCTTTCATGAGCTTGGGATCTCTCAAACTTTAATTGAAACGCTTAATGCGCTTCATATTAATAAGCCAACAAAAATTCAACAAATCTCTATCCCTCAGTTTTTATCAGAAAAAAACTTAATAGTTCACTCGCCAACAGGAACTGGTAAAACTGCTGCTTTTGCAATTCCCATAATTGAGAAGCTATTAAAAGAAGATCAAACAGCAAAACCAACTTTAGTAATTGCTCCAACAAGAGAATTAGTAGAACAGATTAAAACCACATTTTCAAATATTGCTAAAAATAAAAAACTAAGAATTATTAGTTTAATTGGTGGTGTACCTGCTTGAAAACAAATCAAAAAAATCAAAACAAATCCCCAAATAATAGTTGGTACTATGGGTAGAATTATGGATCTTTTAGAGCGTAAAGCAATTCATTTTAGCGATTTAGAACACCTAATTATTGATGAAGTTGATTTAATGTTAGACCGTGGTTTTAAAAAACAAATTTTTAATTTACTAGAACAAATCAATTCCTTTAAACAAATTGCTGTTTATTCAGCTAGTTACAACCAAGAAGCTATTAACATTGCCAAGCAAATTACTAATAATGGGATCTTTATTGGATCACCTGAATTTAATAAAGACGCAAATACCAATAATGATAAACTAATCAAACAATTTGTTTGTTATCTATTTTCAGATCAAAAAAAGCAAGCTTTATACAGCCTTATAAAAACAGCACAAGTTAAGTCAATCATTGTTTTTTGTGACACTAAAAAACTAGTTGATGATCTTCATGTATTTTTAAGAAAAAATGAATTAAGAACTTTTGCACTTCATGGTGATAAAAAACAATTTATTAGAGAGAGAAATCTTAAAATCTTTGCCAATACAAAACAACCCACGATTCTAGTAACTACTGATCTTATTGGTCGTGGTATCCATGTTGAAGCAATCGATATGGTTATCAATTATTCAGCTTGTTTAAATCTAGAAGCTTATATAAATAGAATGGGAAGGACTGGCAGAAACAATCATAAAGGGACATGTGTAACTTTCTGCACCTCACAAGAAAAGAAAGTCTTTCTGAAAATGGTTGAGAAAATCACTGATAATCGAATAGCTGAATGTAAACAAATGGAAATAAAGTTAATTCCTTTAAAAAATAAAGCTAAAACTAAAAAAGGTGGTATTTCACTTGATTGTGTTCAGAAAATATATGCCAATGCAAAACCATATGACCGTAATAAACGTGTCCCTTTAGCAAGTGATCTTTTCAAAAGTCGTATGCGCCAGCCTGAAAAAGCTATGCAAAAGCAAAAAATTCATGACAATGACTGACAAAGTAATATGTAATAACAATTTATAAAATACTTGTATGGCAAAAAAAGACCAACTTACCTTAAGAGGGCCTTTGTATGGCAATAATCGTTCTCATTCCAAAACTATTACAAGAAGAAAATGGAATGTAAACCTTCAGTCATGCAAAATTAAAGATACTAATGGTAAAGTAACACGGATTCTAGTTTCAACTAAAACAATTCGTACCCTTAAAAAACAAAATCGTTTCTAATTTAAATTTAAGTTAAACTTAATCCATAAGCATATATGGATAAAAAATACGATATCACAGCTGTTTTGAACGATGATAGTTCTATTAATGCAGTAAGCGATAACTTCCAAATAACACTGGATGCACGACCAAAAGAAAAATCTAAAGGCATTAATCCTCTGTCAGCTTTTTTGGCTGGTTTAGCTGCATGTGAACTTGCAACTGCTAATGCGATGGCAGCTGCTAAGATGATAACTTTGAACAAGGCACTGATTAACATCAAAGGTTATCGTTTAACAAATCCTAGTGATGGTTATTTTGGCCTACGTGAACTTAACATTCACTGAGAAATTCACTCTCCTAATGAGGAAGAAGAGATTAAAGAATTCATTGATTTTGTAAGTAAACGTTGTCCTGCTCATAACACTTTGCATGGAACTAGCAATTTTAAGATAAATATTAGCGTTACTTTAGTCCACTAAAACTTATTAAAAAAGCGAAAAATACCTGTAATTTTGCAGGTATTTTTTTATTTAAAAAAAGGATAAATTTAAGAAATTAAATTTCGCTTTAGCAGTCGATAATTGATCATATGAAAAATAATATTAGTGATGTAAAGTTGGGACTGTTAGCAGCAAAAATTTATTGAAAATCTTGACGCTTTTTAGAGTTAACAGAAGATGACATTATCTCTATTGCACTTCATGCAGAGCAAGATTCTAAGAAGCGTTTTAATCCTGAATTTGGCTTAAGTTTTGACAACTATCTCAAATTAAATGGAGCAAATTTCATTAGATCAAGTTTTAGAAGTATGGTGAATAAAGTTGAATTGCTTGATTCTAAAAGTAAATACTCATTAGAAAAGCAAAACACAGTTCTAAATACACCTGAAAACTATTTACGGAGTTTAGAATTTAAAGAAATTATTACTAAAGCTTTTAATAAAGCTAAAAACGATCAAGAGAGAAAAGTTTTTTCTTTATATGTAAAGGGCTATAAAAACTTTGAGATAGCAAAAAAGCTCAATATTAGTCCTAGAAGAGTGAGATATTTATTAGATCTTTTTAAAAGCTACATCAAATTGCTAACAGAAAGATATGGATATTAAATAAAGATATTTTTCTTTTTAAAGAAATTTTCTACTAGTTTTCTAACAGCACTATCACTAGTTAATTTCAATGCTTTTTCAACTAATGATTTACATTCATTAATTGTTATTTTAGCAATAACCATTCTTGCTTTAAACATTGAACTTGCACTCATTGATAACTCTGTTAACCCTAAACCTAAAAGCAACGGTATTGCATATTGATCACTGGCCATTTCACCACACATTCCAGTTCAAACATTATTTAATTTACCACCTTCTACAACTAGTTTAATTAAGCGTAGTAATGCTGGATTTAATGGTTGGTATAGATAACTAACGTTTTTATTCATCCTATCAGCAGCAAAACTATATTGGATTAAATCATTGCTACCTATTGAGAAAAAATCAACATGTTTTCCTAAGCAATCAGCTGCTAATGCTGCTGATGGAATTTCAATCATTATTCCTAATTTAAATTTCTTAGTTTCATTAAATTCTTGTTGAACTTTTGTTAAAAGTTGCTTAACTTGAACTAATTCATCAAGAGTTGCAACCATTGGAAACATAATTCCTAAATTTCCATAATCAGAAGCTCTTAATAAAGCACGAAGTTGGGTCTTAAAAACAGCTTGTTTATCTAATGTTAAACGGATAGCACGATAACCTAAGAAAGGGTTATCTTCATGAGGAAATTGAAAATAATTTAGTTTTTTATCTCCACCAATGTCTAATGTTCTAATTATTACCAAATCATTTTTAGCTTTTTGTAAAACAGTTTTATAAGCTTCAAATTGAACTGATTCATCAGGTCAATCTTGACTACTCATATAGAGAAATTCAGTTCTAAAAAGACCAATTCCATTCGTGTTATACTCAACTGCTAAATCCATATCTTTTACATTACCAATATTAGAAGCGACAATAACTTCATATCCATCTAAGGTTTTTACTAATTTATTGGTATATTGTTTTAATTCATTTTGAAAGTTGGATTCCAACTCTTTTTCTTGTTTTCATTGAGTTATGTCTTTACTTGAAAAGTCAAAACCCACAATACCTTTTCTACCATTGATACCAACTGTTTTGCCATCTTCAACTTTACTGGTAATATTTTTTAAACCAACTATGGCAGGAATTTCCATAGAACGAGCCATAATGGCAGCATGACTTGTTTTACCACCACTCTCAGTTAAAAAACCCTTGACATACTTTTTATTAAGAGTTGCTGTTTGACTTGGTGTTAAATCATTAGCTACTATAATGACATCACTTTTAATCCTGATTAAATCATTAAGTTTTACTCCAGTTAAATAACTTAAGAGCCTTTGGTGTAAATCTAATATATCACTGGCACGTTCTTTAAAATACTTATCATCCATTTCACTAAACATTAAAGCTGTTTGTTGAAAAACATTATCAACAGCAATAACAGGATGGATGTTTTTATTTAGTTGTTGTTCTAATTGCTCAGTGATAGTAGGATCATTAAGAATTTGGATGTGTGCATCAAAAATCATTCCTGCTTCTTGATTAATATTCTTAACAGTAATTGTCTTAATCTCTTCAAGGTCTTTTTTTGCTTTTTGAAAAGCACTGCTTAAAAGCTTTTTTGCTTGGGTGGGTGTCATTTTGACATTTGTGTATTTTTTAACGTCAAATTGAGGTGTTTGAATAATGAAAGCTTTTGCAACAGCAATGCCATCTGAAACACCAATCCCAATTATTTTTTTCATAGGTTAATTGTTATTTTTTAATAAGGATTTTGCAGTCATCTCTTTTGGTTTGCTAAGGTTAAGATATTCCAAGATAGTAGGAGCAATATTAGCTAAAATTCCAGTTTGATTAAAGTTAACATTTTTGTCAGTACATACAAATGGTACAGGATTAATAGTGTGTTTAGTAACTGGATTGTTATTATTATCAATCATCACTTCTGCATTCCCATGATCTGCAGTTAAAAACATAGTTATTTGATTAGCTTTACAAAAATCAACTATTCGTTTAATTTGAACATCGAGTGCTTCAAGAGCTTTAATGCAAGCTTGATAGTTACCAGTATGACCTACCATATCAGGATTAGCAAAATTTAAAACAGTAAAATCAAAGTTATTAAGCTTTTCTAGTAGTGCATCAGTAATAGCTTTACATGACATTTCGGGAGCTAAATCATATGTAGCAACTTTTAAAGAAGGAATTAATGTCTTTGTTTCATTGCTGAGATTAACTTCAAAACCACCATCAAAAAAGAAAGTAACGTGAGCATACTTTTCAGTTTCTGCAATCCTCAATTGCTTCAAATTATTATTAGCAATTACTTCACCAAGACTATTTTTAATGGTTTGAGGTGGAAAAGCAAATTCGCTAGGTACAATTCCCTCATAATTCATCATTGTTACAAAAAATAAATTTTCTTTTCGTTTCAATTCAGGTTGATAGTTGTAATAATTGCTGTTAAAGATCAAATGGGACATTTGTCTTGCTCTATCAGGTCTAAAATTAAAGAAAATAACTCCATCATTATTGTTTAATGCAAACTGATCAGAATTTAAATTGGCATTAATTGCAGGATAAATAAATTCATCAGTAATTTGGTTCTGATATTGCGTTTCAATATAACCAATTGGGTCATTGAATTTATTTTTTGAAACTCCTAATAAAGCTTTATAAGCAATCATTTCACGATCCCAGCGTTGATCACGATCCATTCCATAGTATCTTCCCCCAATAGTTCCAATAACAACATTAGGATAGTTTTTTAGAAATATCATTAATTTCTCAAGATCTTGTTTTAAGCTACAAGGTGCTACATCTCTACCATCACCAAATAAATGTAATACTACCTTTGCATGTTTTGAAAACAATTCAATGAGTGCTAATAGATGTTCATTATGACTATGCACTCCTCCATTGGAAAATAACCCAATTAAATGGATTTTTGAATGGTTTTTTTCTACATGTTCTATGGTTTTTAAAAAAGCTTTATTTGCAAAAAAACTACGATCCTTAATATGTTGATTAATCAAAGAAAGTCCAGTATAAACAACTCGACCTGCCCCAATATTTAGATGACCTACCTCAGAGTTACCTATTTGACCCATAGGCAATCCAACTGCTTCCCCAGATGCATCTAAAAGTACACAAGGATATGAATTGATTAATTCATCTAGCATTGGGGTATTTGCATTTTGTACTGCATTACCATAAATAGCATTTGAGATCCCATAACCATCAAGGATTGCTAATAAAACTTTTTTATGCATATACCCTAGCCATTTCTAAAAAGTTTTTAATTTCTAAAGATGCTTTACCAACTAAAAATCCATCAATTTGTTCCATTATTGCTAGTTTTTGGATATTATTATGATCAACTGATCCGCCATATAGAATTGAGATATTGTTAGCAACATTTTCATCATATAAGTCATTAATATATTCCCTAATGGTTTTAATGGTTTGATTTGCAACTTCAGGAGTTGCTGTTTTACCTGTCCCAATTGCTCACAAAGGTTCATAAGCAATAACTAAATTTTTAATTAAGCTTTTGTCAATCGTATCTAAGCAATTAGTAAGATCAGTTTTAAGAAAGCTAATCTCTTGTCCTAAAGCCTCACCAATACATAAAACTACTTGCATGGATGCTTTTAGACAAGCAAAGAGCTTTTGATTAATAACTGCACTGGTTTCGTTATAGTATTTTCTTCTTTCAGAATGACCAATAATACTGTTGTTAACACCAATGTCTTGAAGTTGAGTAAAGCTTACAGTTCCAGTATATGAACCACTTTCAATAAAGTTAGCGTCTTGGGCAAATAAAAGGAGACTATCACTAATTATTTTTTTTATTTCAGTGAGATGAACATAAACAGGTGCTATCCCAATTTTGGCATTGTAATTAAGTTTATTTTGTTGAAATTGTTCAACAAAACTAACTGCGTCTTTTAAATTTTTATTTGTTTTCCAATTGCCAATTAAATACCTTGTGCGCATATGAAAGATTTTATAAGTTTTCAAAAACCATTTTCTTTTCCAACTAATTGATCATTAAGTTATGACTTGTTTTCAGTTCCATGTTTTGTTTGCTTATTGGTACGTCTATCATAGATATAGCCATCAAGCTTTTTAATAAAAGTAATGGAAAACAAAGCATCACGATCAAACTTTCTCACATCTTCTAAAAGCTTAGCAGCTTTAATAAGCAAACAATTAGTTACAAGCACTTGGGTTTTTTGGCGTGAATATCCTCCTTCAGCTTCAAACATTGTGATTGAAAATCACTGTTGATTATCAAGTAGATAGTTACGAATTTTTTCTATGTGCTTACCATAAACTTCTACTTTTACAAATTGATACTTAGGGAAATAAAAAGAAGTAAATAATCCCAACACAATGTTCATCAATAGAGTAAAAACGAGGTTTGGAGATAAAAAGAAAGCAACTCCAAATGGCTGGTGTTTATCATCACCAACATAAGATTGCACTGATAAACTACCAGTTAAATAAGTACCAATTACATAGCCAATAATAAAGCTAACTGTATTAATTAGCATCAAAATTCCACCAATGTCTTTGTATTTTTTTTCAGAATATCAGAATGCTAAAAAGTCAAGACCGCCTGTAGAAGCGTCAATAATTAAAATAACTGAATAAAAAACTGCTTGTAAAAACCCCCAAATAAGTCCATAAAAAAGTAAAGATACTTGTTTTTCAGCAGATTTTTCTCAAAATATAAGTTGTACTCCCTTTTCATTAATTAAATTTTCAAATCCACCATCTTTTGCTGTAGTTAAATTTGCAAACAAAAAGAAGTTATCTATTCCAGGGATGTATGAAAAGAAAAAACCAAAAAGATTTGAAACAGCTACAAAATAAAGGGTTAATAGGGTAAATTTTTTGGAGATTTTAAACCATCCAAAAATAAAGAAAGGAACATTAAAAAGAATTTGTGTTAACCAAAAAATAGCATTAAATATAGTTGCTGAATCAACATTAATATTTTGTGAAGTAATAAAAAAATTAACAAGACGAGCTAATCCTTGGCTAATGGAAGCCATACCAATGTCATAAAGTCCTGAAAACTGAACAAAAATAACACCAAGTAATCCTCAAAAAAAAGCAACTATTGTCAAAATTACAAGTTGTAAATAAAGCTTTTTAAGGTTGTATATAGATTGAAACTTAAGAAATGATCCAGAAAGATGAATCCTTACAAAATTACCTGAAATTTTGATGTTTTTATCTTTCATAGTTAAGAACAATTATTTTAATAATTTTGGTAATTAATAATTTCTTATTTACTATCTTATTAGTAATATTAAGCTTAGTGCAATAATGGCAACGAAAATAGAGCTAATAAAAGAATTGCGTAAATCAACACAAGCAAGTGTTATGGATTGTAAACAAGCTTTGGAAAAAAATAATGATGATTTTGAGAAAGCTGTTAAGTGATTAAGAGAAAATGGCATTGTTAAATCAACCAAAAAATTAAATAAGGTTGCAAGTGAAGGAATTATTGTTTTAAAAAGCAATTTACACAAGGCAATTATGGTTGAGATAAACTCACAAACTGATTTTGTAGCCAAAAATCAAGAGTTAAAAGAATTTTCAGATTTAATGCTTGAAAAAATATTTGAAAAAGTAAATCCAAAAACAGAATTAGTTGAAATTGAAAAAATTCAAATTAATAATGATGAAAAAGTTAGTGAAAAACTAGCATTAATTGCTTCTAAAACTGATGAGAAAATAGTACTTAGAAGAGTAGTTGTATTTGAAACTAAAACTAATCAAATTTTCACCTATTTACATGCCAATAAAAGAATTGGGGTAATTATTGAGATTCAAGGAAAACTCAACGAAGATGATGGTAAGCATTTAGCAATGCATATTGCTGCTAATTCACCACAATTTATTGATCAAAGTGATGTTAATCAAACATGACTTCAAAATGAAAGAAATATTATCCGTTCCCAAGCAGAATTAGAGGTTAAAGAAAATCCTAAAAAAGCAATTTTTTTAGAAAAAACTATTGAAGGTAGAGTTAACAAATTACTAATTGATACCTGCTTAATTAACCAAAAATACTTAATTGATGAAACTAAAACAATTGGTCAATTTTTAAAAGAAAAACAAGCTAAGGTTCTTAAATTTATTAGGTATGAAGTGGGAGAGGGGATTATAAAGGAAACTGTTGATTTTGTTAGTGAAGTAAATGCACAAATCAAACAATAAAATCCGCCAAAGAATAATCATTAAACTTAGTGGTGCTGGGCTAACCAAAGAAAATTCTCAACCCTTTTCTAATGATTTTTTTGAAACTATTATTAATCAATTAAAAGTTTTAAAAGAAAGCTATCAAGTAGGAATTGTTATTGGTGGGGGTAACATTATCAGAGGTAATAATTGCCAAGAATTTAACATTGCTGAATACCATGGTCATCAACTTGGTATTATAGCAACAGTAGTTAATGGCTATTTTTTAAAAGCAAAGTTAGATGCACATAATTTGAAAAGTGCTTTACTAAGTGCAATTAGTTGTCCTAGTTTAGCAGTGCAAATTCTTTCACAGCAAACTATTGATAAAGCTTTTGAAGAGAATGACTTTGTCATTTTTTCAGGTGGCACTGGTAATCCTTATTTTTCCACTGACACTGCATTAGCTTTAAGAGCAGTGCAAACAAAAGCAGTTGCTATTCTGATTGGAAAAAATGGTGTTGATGGTGTTTATACAGCTGATCCTAAAAAAGATAAAAATGCAACCTTTTTACCAACACTCAACTATGACCATGCCATTAAAAATGATTTGAAAATTATGGATATTACTGCTTTTACTATGTGTAAGGAAAATAATCTGAAAATAATTATTTTTAACATTAATGCTGAGAATGCATTATTAGATGCATTAAACAAAAAAGGTCGCTTTACTATAATTGAAAATAACTAATGACAAAAGCACATTACATTGATTTTTTTAAACAAGCAGCTGATAAAAAAATTCAATGATTAAAAGAAGAGTTAACAAAGATTAGAACAGGTAGGCCAAATCCTAAAATCTTTGATAATCTTTTGATTGAAAGTTATGGACAAAAAATGCCTTTAATATCTTTAGCTCAAGTGACTATTAATCCGCCAAGAGAAATAATCATAAAACCATTTGATCCTAAGAGTAATACTAATGCTATTTACAGTGAAATTCAGCGGGCAAACATTGGTGTTCAACCAGTTATTGATGGTGAAAAAATTCGTGTTAATTTTCCCCAAATTACTCAAGAAACTCGCTTAGAAAATATTAAGCACGTTAAAAAAATAATAGAGCAAATTTATCAAGAACTGAGGGTTGTAAGAAGAGATGCATTACAAATGATTAAAAAAGATAATCACAATGAGGATTTAGAAAACTCTTTAAAAGCTGAAATAGAAAAAATTAACAAAAATTATTCTAATCAATTAGAAGAGATTCAAAAAGACAAAGAAAAAGAATTGCTAACAATTTAAATGAATGAAAAAGCAAAACAATTCATCAAAAAGCGAACTTCAGTATTCATTGCTTTATTAGTTGTATTTTGCTTTTTTCTTTTAATTAGCGCATTTGCTGATGGTTTTAACTTTTGATCACCGTGATCAGCAGATTTCAATTCAAGAACATTAAAAGTAGAACAAGCAAGTGGTGTTACTAGTGTTATTAGTACTGAGATTAATGAAAACTTTAAAGCTGTTCGTTTCAGCTTTAGCATAATCATTATTTTAATTGTTGGGGTAATTGGTTCTCTGATGATTTGAGAGTTGTTCACAAACATACTAAAAAATAAACCAAAACTAAGCTTAAGTTTAACGTTGTTAAATGCTGGAATAATTATTTTTGGGATGATTGGTACTTTTGTTGTTGTTTATTTTTACAAATGAAATGCAACTGTTAATGGTATTTGAACATTAAGTTTTACTCTTTCTGTGGTTTTACTTTGAATAATTTACATTGCTTGCATGAGTAAAACAAGAATTAAGTTTAGCTTACAACTTTCATATAGCTTAGGAGCTATTGCTTGCTTTATTGCTAGCATAGGTACTATTTACTTTTCTGTTATCAGGGGTTGAACTACAATCTTTTTATTGATGAGTTTAGCAGTCAGTGTTGATACATTTTCTTTTCTTTTTGGAAAGCGCTTTGGTAAAAATCCTTTAATTAAAATTTCACCATCAAAAACATGAGAAGGAGCTTTTTTTGGCATCATTAGCACCATTGTTGTTGTTGCTTTACTTTGTGTTTTATATTCAATTCCTTTCTTTGTAGCAAAGCCTACTTTTAATCAAACAAATGGAATAGCGCTCAATACACCCCAAAATTATGATAGCCATAATCTTATTACCAATATTTTTTTAATTGCCTTTATCTCTGGAGGAAGTAGTTTTTATATCTACTGGTGGGTAAGCACTTTAGCTTTAATTTTTACAGGATCTGTTTTTGCAATAGGCGGTGATCTTTTTTTTAGTTATATTAAACGCTTAATTAGTATCAAAGATTTTTCTAAGGTTTTAGGTAAACATGGGGGAGTTTTAGATCGATTTGATTCAAGTTCTTTTTTAATTAGTTTCTTCTTTGTTTATCATTTAATAGCAGGAACCATTTCCAACCAAAGGTTGTTGATGGAACCTAATACTTATTTCAGTGCAATCACTAGTATTCAAAGCTAGTATTTAGAATTAATAAAGTATGACTCCAAAACTAAAGCTAAATAACAACATAAACTGAACCAAAAGAACAATTGATTCTTTGTTTGATTTAAAAAAAGGTGAAATGCTTGAAAAAGAGTTAATTACACCTGAGGGAAAATATGAATATTTTAACGGTGGTGTAAAAAATTCAGGAAGAACTGACAAGTTCAATACTTTTAAGAACACTATTAGTGTAATTGTTGGTGGATCTTGTGGTTATGTAAGGCTAGCTGATAAAAATTTCTTTTGTGGTCAAAGTAATTGCACATTAAATTTGTTAGATCCGCTTGAACTTGATCTTAAATTTGCTTACTATGCTTTGAAATCACAACAAGAAAGAATAGAGGCTTTAGCTTTTGGGACCACGATTCAAAACATTAGAATTTCAGATTTAAAAGAATTGGAAATTCCTTTCACATCAAATAAGAATGAACAGCATGCTATTGCAAATACTTTAAGTGTTTTTGATGAGAGACTAGAAAACTTAGCTTCTTTAATTGAGATTAACAGGAAACTAAGAGATGAATATGCTCACAAACTCTTTAGCTTAGATGAAGCTTTTCTAAGTCATTGAAAACTAGAAGCATTACAAAGCCAAATGCATGAAATTACTTTAGGGGAAATATTTAATTTCAAAAGTGGTAAATATCTAAAGAGCGAGGAGAGATTAGAAGAAGGGAAATTTCCTTATTATGGAGCGGGAATTGATAACACTGGTTTTGTAGCTGAACCTAATACTGAAAAAGACACAATTTCTATTATTTCTAATGGTTATTCACTAGGTAACATCAGGTATCATGAAATTCCTTGGTTTAATGGCACAGGTAGCATTGCATTAGAACCCATGAATAATGAAATCTATGTACCATTCTTTTACTGTGCTTTAAAGTATTTGCAAAAAGATATTAAAGAAAGAATGAAAAGTGATGATTCACCTTTTTTATCCTTAAAACTAGCAGGTGAAATTAAAGTGCCTTATGTTAAGTCATTTCAACTGCAAAGAAAGGCAGGAAAAATCGTCTTTTTGTTAGATCAAAAATTAGACCAATATAAAAAAGAACTAAGTTCTTTAACAGTGATTCGTGACACTTTGTTAAAAAAATTATTCCCCGATATGACTGAAAGAACTAAATCTATTAAGGATTATTAATCAAAAACTTAATTCTTTCTTTCTAAAATTACTTTAATATCTCTTTTCTTGCAAAATTAGATAATCTTTTCACACCAATTGAATAACCTGATTCAAAATATCCACCATTATTTTGATAGATAAAATTAACAAATACAAAAATTTACTTTCTTTATTTTTAATTTATCGATAAACAGTTCTTACAGCGCCAATATTAGTAACACTTTTTTTGGTTTAGCATTTACTTAATTTTTAGTTTCTTTAACAAATTGAATTGTTATAAAAATGCGATTATTTTCTTGGATTTTAACTGCTTAACTTTTTTTATTTTGATATAAAGTTAACTAATAGAAAAGTATTTATCTTCTTTAGTTTTAAGATCAAAATATATCAGGTTTTATTTTTAAAAAAATTAAAAAATCTTATTAAAGCAATGTGTAATAAAAAAACGGTAAAAGATCGATTGACTTTCCCTTAAAAGTTCTTGTTCTAATTTTCTTCACTTGTTCATTTGTTGAAAGTAATAAATTCAACTTTTCATGAGAAGTACTCATGTCAATTTTGATATCAAAAGGAAAGTTAATTGAAGCAGGTATACTACTAAAAGTACTTTCACTTACCCTCCTATTATCTGTTGGAGGAGTATAAGTAAAGATAACATCACCATCAAAATTAGCTTTATCACCAGTAAAATCACTTTCACTTACACCACTTGTACTAGCTAAAAAGGATTTGATTAATGAAGTACTAGTATTGTCACTACCAACTATACCAGTCATTTTTAAGTGAATTTGAACTTTAAAATCTAAGTTATTTTTTCAACTGTATTGAAAATTAACTACCTTTTGACTTTTAACTCCTAAAACTAAGGCGATTTGACCACTTATACCTGGATAAGTTTCATCAAAATATAAACCTTTTGCACTGATGTTAGGAGTAACATCTTCCTTTGATTTATTAACTCCAAATGCTTTAGCAAATCTGCTATAATCAAGTGGTTTTTCTAATAGTTGATAATCTAATAAAGTTGTTTTTCAACTGGCAAATACAGTATTAATTACCTCTTCCTTATCAGAACTATAACCCTTTTTTAAAAAAGTAACAGCATTATTTTTTGAAAGTTCTATATTGTTTCTCTGAAAATAAGCTTGTGCTTTTTCTAAACCAGGATCAGTTGATGAACAGGCTACAGATAAAGTACTAAGTGAAATTAATGGTAATAAAAAGATCTTTCTGAGTTTCATTACAAAATGGAATAAAGCACATATGGTAAAAGATCGATAGTTTGATCTTGGAAGTTTTGATCAGCTAATTGACCTACAAAATTACTTTGTAATAACTTTTGCATCAATTCATCTTTACCAATAATTTGTAACCTTAAATTGGTATTAAAATTAATTTTTCTCAATAGAGTATCAAAACTAGATTTGGAAAATAAATTACTGGAAGGTGGAGTATAAGTAAAAATTAAATCAGCTGAAATAGAATTTTTATCTTTTATATCACTATCACTTAATCCTATTTGACTCAAATATGATTTTGCTTGAGCACTGTCAAAAGTTCCAGTAGCTTGTAACCTTATTTGGACTTTGAAATCACGAGAACTGTTATAGTAAAAACTAAAATCTGTAACTGTTTGACTACTCAATTTAATTACATTATTAATAATACTTGCAACATTTTGCGTGTATCTTTCAACTAAACGTAAGCCTTTAACACCAGTGCTCGGTTCAACATCTTCCTTACTCTTACCACTGCCAAATGCTTTTACAAATCTACTTGGGTCTTGTTTTTCAAGAATCTTTTCGTCTTGCAAACTAAATCTTCAAGCATCTAATAGAACGTTTGTAGCACTTTTTGAATCCACTTCAAAACTATCTCTTAGTGAAGTAACTAACTCTTTCTTGTCTTGATTTAATTCACTATTTTTACTGAAACTATTTCTAAGTTGGTTTAAACTGGAAAAATTTGTTCCACTGCTTAAGTTAGAGCATGCTGTAATTAAAGAACCTAATACCATTAAAGAAGAAATGATGCCAGCTTTTGTTCAAAATTGTTTTTTTTAACATTGTTTAAGTTAACAGATTTTCTTATTTGTAATTTTTTGATCATTAATAGAGGTAAACTTCAACAACAATCATTTAGAAGTTTTGTTTCTATTTTTATCAATTAACAAATAGAAGGTTTCTATACTTAAAAAAACAAAAATAATCACTTAATTTTGAATTAATAACTTCTTGTTCTTGCAAGCCTAGATTATTGACATGAAACAGTTTTACTGCTTGAAAAGTTTCATTGGAAATCGATTCTCTACTTAATCTAATTCCAAGATTTTGAATTTGTGTTTTTATAAAAAAATGAATATTAGCAATTAAGTTTCAAACTATTTCTGTTTTTTGAAAAAGTCTAACTAAAAGATATAAAGAAGTCTGATTAAACAAAAAATGCTTAAATCTTTTTCCAAAGTTTCTAAATAAAACTAATATTATTTTTAAACAAAAAAGAAACAGTACAAAAAATGCAAATACTGATGCTTTTAAACTAAATTTAGATCTAAGAAAACTAACACTCATTGCGTTTTAAGCAAACTAAAAACAACAATTAAGAATGATTTTTCAAAAGTTGCAACTATAATTTTAAAACTTTTATTGATTTCTTTTGTATTAAAAACATTTGCACAACTTACTCTAAATTAATATCTTTTTACTAATAATAAGAGATTTTTTTAAAACTAATGGAATTACTAATTAAGTTCATAACTTAATTTGCCATATTTCCCATTAATAAGTTCATTAATAAAAATTTCACATGCTAAGTTAGTGTTTAGTTCATTAGCTTTCTTAATTAAGCCTCTCACTTTAGCAAATTTTTCCAAAAAGTTAATAAAGCTATCCGCTTCAAATGGTAAAAGCTGTTTGTAATGTTTCTTAAGATAATTAAAAGCAAACATTCCCACTTCTTCTATATTAACTACTTCTCTTCTAATAACATTAGTCAAAACTAGTTTATATCCAATTTGAATTTCATCAATTCTTTTCAAAAAAACTCCTGGGGTATCACTTAATAATAATTCAGGGCTAATTTGAATCCAATTTAATGATTTAGTAATACCAGCGCGGTTAGCTACTTTTAAGTGATTTTTATTTATCAAAAGGTTAATTAAACTAGATTTACCTACATTGGGCATGCCAATAACTGCTAATCTAAATTGTTTGATTAATAAGCCTTTTGCTTTTAATTGTTGTCTTTTATTTGCAAATAAAGTTGTTAATGTCTTTAGAACTTTTTTTTTGAGTTTAAAAGGTTCTTTTAATGAACCAAATAAGATCTTTTTATTTGGTTTGTATTGTGCTAAATCTGTCTTTAATGCCAGTATTAATTTTGGTTTATTTAAAAAATAACTAATTATTTCTGAATTATGAGTTAAAGTTGGTGCTCTAGCATCTACTATCTCAATGATGCCATCAATTTGACTAGATAACTTTTTTAATTGATCATGGATCTTTTTCATGTGACCCGGGAACCAATTAATTTTGGCGGAGGTATATGTGTCCATCTATTCCCCTGATTCTTGTAATAGATACCAAACAATCCTTGTCAATTTGTCTAATAATCTTAATTAAATGGGGAATTTCTACATACATGGAAACAGAAACTATCATCTTCTTTTTGAGTAAAGAATAACCACCAAGCGTTTCTTGAATAGATAAACTGTGATTAGCATTATCACTTAACAATGCTTTTCTAACTTCTTCAAGCTTATCAGTAAATACTTTAATTTCAGCAAAATTATAACGAGGGAAAAGATAACTAACTACTGTTCCTGTTAACAAAATTGAAAAAAAAGTTGCAATTAAATTAGGTGAGAAAAATAAACTAACTTCCCAAGCAGAATCACGATAATTATTGACATCTTGCAACAATAAACTACCAGCAACAAAAGAACCAATTAGAATAGCAATAATCAGAATAAAACTATTTACATAAAAAAGAATTGATCCTACTGATCTATTTTTCTTACGAGCATAATATTGGGTTAAGAAGTCCGCTCCCCCAGCAGAACCACCTAGTATGTAAAGTAATGAAACTGATATGCCATTATAAAAACCATAAATAGCTGCATAAATAAATGTGGAAATAATTACATTACCTTGGGAAGTATCACTTCATAAAAAAGGGACAAAACCGGCACTTTGATTTAAATCTTTCGCTGCTTTTCAAAAGTTAGTATCTGTTATAGAAGCTAACATTGGTGGTAAATTATCAGAACCTGGAATAATACTGATTAAAAAACCAAATACGTTAGATGCAACAACAAAATGGGTTGAAAGAATAGTAAAGTTTTTACCTATTTTTTTATAAGAAAAAATAATTAAAGGGATGTTGATAAAGACATATAAAAGCCAGTAAAGGATGTTAAAAATTAATAATCTTTGAGTTTCATCAAAACTTTTTAACAAAACAAACACTAATCTAGCAAAACCTTGAGTTAAAGAACTAATTCCAAAGCTATATAAACCTGTTTTTTGAATAAAAATAAGTAAAAGAAAAGCATTAATAATTGATAGTAAGTAAACTAATACATACTTTAATGGTTTTTTAGCCTCATAAAGATTGCTAAACATCAAAAAAGAGTTAGAAATCCTTACGCGTTTAGAACCAGATGCGACTGTTATCTTTGATTCTTTTTTAAATAAGTTGTTAAAAAATTTCATTTGTAACAAACTTTTTTAAGACTTTTTTTCTTTAATTTTTGCTGACTTACCAGATCTTTCACGCATATAAGAGATGTATGCTCTTCTTACTTTACCCCTGCGTTTTACTTCTATGTCTATATTAGGGTTGTGGATTTGAAAGTTTTTTTCAATAGGAATTCCATCAGTGGTTTTTCTTACCATGAAAGTTTCGCTAATCCCTCTTCCCCTTCTTCTTAAAACAGTTCCAGTGAAGTTTTGAACTCGAACTTTTTCTTTTTCACGTAACTTAATAGCAACATTAACTTCATCTCCTGCTCCAAATTCAGGAACATATTCCTTTAACTGTTTTTGTTCTACTGCATCAATTAATGCTTGTTTATTTATTTTTTTCATTTTTATTTTCTAAGTATTTTTTGTATAAATCAGGTCTGTATTTTGCAGTTTTTAAGATTTGCTGTTCTTTACGAAATGATTCAATCTTTTGGTGATCTCCTGAAAGTAAAACTTCAGGAACTTTATCGCCTTTTAAATCGTATGGCCTTGTATAAACAGGAAAATCTAATAGATTATCATTGAATGATTCACAAATAAGACTCTGATCATTAATAACTCCTTTAATTAATCTAACAGTAGCATCAATAACACTTAGTGCTACAAGTTCCCCACCACTTAAAACAAAATCACCTAAAGAAACAATTTGATCAATATATTTATAAATTCTTTGATCAAAACCTTCATAGTGACCAGATAACAAAATTAAGTGTTCGTATTTTGTAAGTTTTTTAGCACAATTCTGAGAAAATTGTTCACCTTCTGGGGAGAGTAAAACTACATGAGAATTTGGGGCTTTATAAAAATTTAAACAATTAATAATAGGTTCAGCTTTTAAAACCATGCCACTTCCTCCACCATAAGCCATATCATCCACAGTTTTATGTTTATCATTGCAAAAATTTCTTCAATTTACTACTTCAAATTGAACTAAATTTGCTTTTTGAGCTTGTAACATAATAGAACTATTTAAGTAAGGCCAAATAGTGTTTTCAAAAAGTGTTAAAACAGTGATTTTCACTACTTTTTAGGACTTGTTTCTTTCTTATTTTTACTTTCAATAAATTTCTTCCACAAACCAGATTCACTAAATAAAGAACGGACTGTATCAGTTGGAATTGCCCCTTTATTAAGTCAATCTAAGGCAACAGTTTCATCTAATTTACACTTATTTTCCTTTAAAGCTGGATTTAGATGTCCAATTAAAGCGATATACTTTCCATTACGCTTTACTCGCGAATCAACCGCTACTATTCTATAAAGCGGATAGTGTACTCTTCCCATCCGCATCAATCTTATTTTGACCAATGATTTTTTTCTTAATAAAGCGGCTAATTATAACTTTTTAAAGTTCTATTTTATCTATGCTTAAGAATAACTTAATCAGCTATTTTTTATTCGTCTAGTTAATAAATTTAATCATCCAATAAGGCCCGATTTTCATTTTGCATGTCATCTGCAAACTCATTATTTTTTTAGATAAATAAGTTCTTATCTTTATATCTAACAAAGTCTTAATTATTTTCTGTTGTTTAGCATATTATTTTAAGGCGTTAAACTTGTTTTCAGAATTAATGATGAAATAGTTGTTTTGCTATTCACAATAGCAAAACTTAAATTTGAATTATGTTTTTGTTTATTAATCTGTTCAATATGTTCTTCTCAAATTAACCTATATTTTTATCGGTTAATTCTTTTTTAATTTCATATAAGTAGATTAATGTAGTTCCACTATTAACAAATTTTTGTTTATTTTTAAGGAATTCCAATAGTTTTTGTCACTTTCATTTGGGAGGAGAGATTTACCATTTTTAATTGAATTAATTACAAAATTTTTTTTAACAAATAATTAGTTGTTTAATAATCGAAATTATTAATTAGTTTTTTTAGCATTCATACTGTAATTGGATTTTTCTAATTATGAGTACTTTTCAATAAATTTGACCAACTTATTTTGTGGTGGATTAGCGAAATATTTATCCATAAACAGATTAGTGTGAATTTGACCAAAAGCAATATTAATTAAACCTGCTAAGGCAGCATTTGCAGTTAACAATCAAATAAAAACTTCAATGTTATTTGTTTGAAGTGTTATTGCTTGAAAAATAAAAAGTAAAGGAATAAATACAAACAATCCGTGTGTTAAAGAGACAATTCAAGAATAAAGCACTCTGTTAGTATTTTGAAAATATTGTTGACTATTAGCGCCTGTGGCTACAAAGAAAACTTGTGCTTGCACTATTAATGAAAAGTAATTTGCTAAATCCAAATTTTGATCTGAAACATCAAAAAAACTAGATAAGATCTGTTTTCCAAAAGCAACTGCTGTTAACAAATAGATTAAACTACCGAACGATATACAGATAATAACTGTATAAATGTTAATTTTTTTAATTTCATCATATTTTTTTTGTCCAAACTTATATGATGAAACTGTTCTAACTCCTTGTAATAAACCAAAAATAGCTGCTGAAGCTAAATTACTAATAGCAATAGGTCCAGTTAAAAGTGTCAGATAAAAAACATCATTTTTATCAGTAGTTGCTTTTGTCAAATTAACCAAAAAACTCTCATAAAATGTAGTAACAATAGAAAGTGATCCATTTCTAAAAAAGGATGCCATTCCTATTAATGAAACTACAACTAGTAGATTAAAGTCAATTTTGTTTAATTTAATAGTTTTAAAAGTTAAATAAGTAAGATTTCTTTTGTTTAAATAAATTAGATAAATAATATAAGCTAAAAAATTAATTAAGTATCCTAGTATTCCAGCCACAGCTGATCCTATTACTCCTAAAGAAGAATATCTAACTAATAAAAAAACTATTAATATGTTAATCAAATTAGCAATAGGCGGAACAATTGCAATAAAAAGTTGTCTTCCCTCTGATTGTGCTAAATAAAAAAACAACCTTGAAAGCATAGGAATGATGTTAAGACCAATCAAAATATAAACATATTCAGATGCTACATCAATAGCCTTTTTGTTAAAAAACTGTTGGAAGCTATTAGCTTGAAAATTTTGTTCAAAACTTGATTGATCAAGATTATAATGCAACCATTCTTTTGCAAAAGATAAAACCAAAAATTGGGTAATTAGTCCAAATACAGTTGTTGATATTAATCCGGTATTTCAAGCTTCCTGAATTTTATTTTGATCATTTCTACCAATTGCTTTAGAAAAAATAACTCCAGTTCCTAGAGGAATAAAAATATTAATGGCGTTTAACAAAACAATCAAAGGTTGACTAATATTTAAAACAGTTTTAATAAATTGTTTAATATTAAGTCCAGAGCTTAAAAAACTATCACTTTTATAAAAAGCAGAATTTTTAAATTCATCAATTAAACTCTGATCAGTAAAAATAGAATCAGGATTAAGTTCACTTCTAGGAACAAACTTAATCACCATGATTTGATCTATAAATACATAAGCAGCACTAAAAAGTGCAAAGAAAAAAGTTGGAACAGTAAATCTAAGAATAGTTAGAAAAAGCTGTTTTGATTCAAAAACATTTTTAATTAGGAGTTGTTTTTCAAAACGATCCCTTTTACTGTTCACTTATTTATTTTTAAAAAGATGGATGTAATCCTTGTATCCTAATTTTTCCATCTCTTCAAAAGGAATAAATTTTAAAGCTGCAGAATTAATACAATAGCGCAATCCACCTAATTCACTCGGTCCATCATTAAAAACATGTCCCAAGTGGCTATCACTGTTTTTAGCACGAACTTCAGTTCTTATCATTCCGTGAGATTCATCACGGTAGTTAGCAATTAAATTTTTATCAATGGGTTTTGAAAAAGCAGGTCAACCACAACCAGATTTAAATTTATCAGTTGAAATAAAAAGTGGTTCACCAGATGTTATATCAACATAAATTCCCTTTTCAAAATTGCGGTTATATTCATTAATGTAAGGTGGCTCAGTGTGAGCATTTTGCGTTACATCAAACTGGAGTTTGGTTAAAGTACGTTTTAGCTCAGCTTCACTCTTTTTTTGGTATTTACTCATTTGGAATGTTGTTTATTTAAATATACATCAATAAATGGTTTTCCTACTTTGATCTTAGGATTAATCTCAATTATACCCTCATTTTTCTGTTTTAAATTAAGTTCTTTTTCAGAACATAACATGCCAACTGAATCAAAACCAGCTATTTTAGTTTTTTTGATAACTGTACCGCTAGGTAACACTCCACCTACTTGTACTAAAACTGTTTTCATACCAACTCTAACATTATTAGCTCCACAAACAATATCCAAGCTTTTTGTCAAACCAGTGTTAACTTTACACCTTTTTAGATGGGTGTTTGCAATAGGAATAACTGAAACAACTTCACAAACAACAAAAGGAACTTTATTTGCTAATGAAATAAGATCATAACCTAAAAGCTCACTTATTCTTTTCATTATTTTTAATGAAGGATAGTTTAGACCTTCATTAAAGCGGTGTGATAAAAAAACTTCTTAAAACTTTTTTTGATATTAAAAAAGTTAAAACCAGTAATTCTATTTGCATCATTAACAAAAAAACTTCAGTCTTTGTCATTTTGTTGGCGTAAAGTTGTTTCACTTCTACTTCCTATGATGCCAAACATACAGTTTTTTAATGTTTCTTTACGATAAAAGATAGAAACAAAATCTTTAGATATATCAAACATAAAAGTTAGTTATTCTGTTTGTATTAAAAAGGCATAACTATTGATACCTGTGTGTGTACAAATTACAGGTGAAAGCAAGTGCTCACTTACATCAACTGCATTGTTGAGAAGCGTAACAAATTCTTGCTTTATGAGTTGATTTTTATCAGTTTCAAACATTGTTGTCAACAAAGCCGCTCTTCTGAAATTAACTTCTTTTTTTAATAGCTTTTTTTCTAAAAATTGAACTGCTAACTTATGAGCACTGCTAGCAGATTGTGCTTTGTTAAAAAACTGCAATTTGCCTGTTTCACCAAGGAAACTAATTAAGAGATGAAATTTAAAGCTTTTGATAATAAATGACTTTAAATTAGATAATCTTCCTCCAGCTACTAACGGTTTTGTATCAGTTACAAATAAAATTGCACCGCACTTATTACGGAAGTTATAAACAAATTCATCTAAGCCTGCTCTTGAATACTGGTTGTTTTTAAGTCAAACCTTAATATCACTAATCAATCAAGCTATTAAAACTTCAACTATTCTGCTATCTAAAACATGAAACTTGTCTTCAAATTCCTTTTCAACAATCTTTCAATTTAAATAGGAGGTTGAGATCTCTTTATCAATAGGGATTCCTATGATAAGATCATAATCATTAATTATGCTCTTAACTTTGTCACGAACCTCTTCTTCAGAAGTTTGTGATGTTGAAAACTTAACACCTTTAGGGGCGTTAATCATCTTATCAGTTAAGGTTTTAAGATCAATGTTAAAACCTGATTTGAATGTTTCTTGGTGTTCAGCAATTGTTTCAATAATATAAAGCGGTAAAACATAGAGATGACTATTTTTATCTTCCTTTAGATTACTGACAGAATCAACTAAAAAAGCGATCCTCACTATTCTAGAACTTCTGTTACAGTGCCTGCCCCTACAGTTCTACCACCTTCACGAATTGAGAACTTACTACCTTTTTCACAAGCGATAGGAGCAATTAACTCAACAGTAATAGAAGCATTATCACCAGGTAGAACCATTTCAGTATTTTCAGCTAAAGCAATAGAACCAGTTACATCAGTGGTACGGAAATAGAATTGAGGACGGTAACCGTTTAAAAAACCAGTGTGTCTACCACCTTCTTCTTTCTTTAAAGCATAGATCTCAGCTTTAAATTTCTTGTGCGGTTTAATAGAGCCTGGTTTTGCTAAAACTTGACCTCTTTCAACTTCTTTACGTTCAACACCACGTAATAATACCCCAGCATTGTCACCAGCCATTGCTGAATCAAGTTCCTTTTTGAACATTTCAATTCCAGTAACAACTGCTTTTCTAATTGGTTTTAAACCAACAATTTCAACTTCTTGACCTACTTTGAGTTCACCTCTTTCAACTCTTCCTGTAACAACTGTACCTCTACCAGTAATGGTCATCGTATCTTCAATTGCTAATAAGAAAGGTTTATCTACTTCACGTGTAGGAGTTGGAATCCATTCATCAACTGCTTTAATCAAATCATGGATCTTAGCCTCCCACTTTGGATCACCTTCCAATGCTTTTAAAGCTGAGCCATAAATAATAGGAGTGTTCTTACCATCAAAACCATAGGAAGTTAACAGATCACGTACTTCTTCAGCAACAAGTTCTTGTACCTCTTCATCACTAGCAATATCACACTTGTTTAGAAAAACTACCATTTTAGGAACCCCTACTTGGCGGGCAAGTAAGATGTGCTCGCGGGTTTGGGGCATCACACTATCAGTTGCTGAAACAACTAGAATAGCTCCATCCATTTGTGCAGCACCTGTGATCATATTTTTAATGTAGTCAGCATGTCCAGGACAGTCAACATGGGCATAGTGACGTTTGTCAGAAGAATATTCTACGTGTGCAGAGTTAATTGTGATTCCCCTTGCTTTTTCTTCAGGGGCTTTATCAATTTCATCATAACGCGTTGCAGCTGATTTTCCTTCCTTTGCTAAAACTGTACAGATAGCAGCTGTTAAAGTGGTTTTACCATGGTCAATGTGACCAATGGTACCAACATTGACATGTGGTTTGGAACGGTCAAATTTCTCTCTTGCCATTTGTTTAAATTATGTTGTTAATTTTAAATAAGTTTAAGATAAGCTGGAGATAATGCTTATAATCTTCAAGGACTTAAATTTAGTGAAAACAAACAATTGCCAAATTATTGGCTGATGAAAACAACTTTCCTTACCACAAAAGACATTCTTAAGTTTCATTCCCTTATTTTTGGTCACTAGTGCTTTTGTTTTAACTGGAATTGTTGAAAGTCTTTTAACATTTGGAACTATTATTGAACAAATTGATAAATTCACTGATCAGACTAATGTGATGTTATTAATTTATGCAGTTATCTACACTTTTAATCCAAAAAGTTGATTGTTAAAAAACCAACAATTCTTTTTAAGTGCATTAGCTTATATATTATTTACTTTTATTGGCTATAACCTAATTTTGTCAATAGCTGGTATAGCTTATAAATCAACAAATCCATATAAGTTAACAAGTAGTATTTTTCTCCATGTAATTGCACCAATAGCATTCTTCATAGCAAGTTTTATCAAAATAAAACATGAGAAAGATGTCAATATTAACATGTTCTTTAAAAGCCTATTATTATTCATGATCTATCCTTTAATATATGGGCTTTATTTAGTAACTATTCCATATGTAAGGCATTATCTTTTTAATGGTAGGCCATCTACTTATACCATTTATGGCAGCATTACAAATACTAAAAATAATCCTTTTGCTTGATTAGTTGTATTTGCAGTTTTATTTATCTATTTCCCCTTGAGTTACTTAGCTATATATCTATTACAACTTAAGTTAATAAAAAAAGCCATACAACCGCAATTTAATTTGCCTTTTACATTAAATAAATGAAAACAAAAATAAGAAAAGCAGTTATTCCTGCTGCTGGGTTGGGTGTTAGGTTACTACCAGCAACAAAAGCAATTCCCAAAGAGATGTTACCATTGGTAAATAAACCTACTATCCAATACATAGTAGAGGAAGCAGTTAAAAGTGGCATTGAACAGATTCTTGTCATTGTTTCATCCAAAAAAACAGCTATATTAGATCATTTTGATTATGATCTGATCTTAGAAAATGCCTTAATTCAAAAAAATAAATTGCAGGAGCATAAAGAGATTGAAGATATTGCTAATTTAGCACATATCTTTTTTGTTAGACAAAAAAATCAAGATGGTTTGGGAGATGCAATCTTGTTTGCTGAATCTTTTGTTGGTAATGAAGACTTTGCAGTATTGTTAGGTGATGATGTTGTTTTTAGTAAAGAACCTGCTTTAAAACAATGCTTGGAAGCTTATTATGAAACTAATTGTCAAACAATCGGTGTACAAGAAGTAGATCCTTGTCATGTTGATAAGTATGGAATTATCACCCCTGAAGGTGATTACAAAAATAAAGATCTTATTAAGGTTTTAGCAATGACTGAAAAACCTAAACCAAAAGATGCTAAAAGTAATTTAGCAATCTTAGGGCGATATGTACTCAAACCATCTATTTTCAAAGCACTTAGAAGTGTACCTTATGGAGTTGGTGGTGAGTTGCAACTAACTGATGGTTTAAATTTTTGTTTGAAAAATGAAAACTTTTATGCAAGAAAGTTTACTGGTACTAGGTTTGATGTTGGCACAAAGAGTGGTTTTATTAAAGCAAATTTATTTACTGCTTTAAACAATAAAGATATTAGTAAAAAAGAAGTTTTAGAACTTTTAAATTTAGTTAAAGCTTAATACCATTTAAAGTTAAACCAAGGAAGTTTTCATTTCTAATCAATCGACTAAAAGGACACATTTCATGGGCTTTTTGAATTAGTTTTTTACCAACTTCTTGGTCATTACTATTAGTAGTTAATTCAACACCAGCCTTAATGTGAAATAGTCCATTTTCTTGATGGAGTTCTACTTTAACACTTACAACTGGTTTTTTTGAAAAACTAAATTGATGTTGTTGCATAACAACAATTACTGCTTGAGAAAAACAACTTGCATAAGCAGATGCAAATAACTGCTCAGGATTATTTTCTGTTTGAACTGATAAATCAGGTTTGGGAAAACTAAGTTTTGTTTGAAAACCATCTAAAGTTTTAACACTACCTTCTCTGCCAGTTTCAGTTTGTGCAACAGTTTTATAAATCAATGCCATGTTAATTAATATAGATAATATTTTAGTAAAAATGTTAAATAACATATTGCAATTTCTCAAAGAAAGAGAACTTTATTCACAAGCTAATTTTGAAACAGAACTAGATAACCATTTAAAAGAGAAAAAAAATAACTTTTATGTTGGTTTTGATCCAACTGCTAATTCTTTACATATTGGCAATTATGTTTTAATTCACATTGCAAAATTATTAAAAGACATGGGGCATACTCCGCACATAGTTCTAGGGAGTGCAACTGCTTTAATTGGTGATCCTACTGGCAGAATTGAATTAAGGAAAATTTTAGAAGAAAAAGAAATTGTAAAAAACACCAAAACAATTAAAAAACAAATCAAACAGTTTTTAGGTGATGTAATTATTCATGAAAACAAAGTTTGATTAGAAAAACTTAATTACATTGAAGTTATCCGTGAATTAGGTGCTTTTTTTTCAGTTAACAAGATGTTAAGCACAGACGCATTTAGTGCTAGGTGAGAAAAAGGACTAACTCTAATGGAATTAAACTATATGATCTTACAAGCATATGACTTTTATTATCTACATAAAAACCATAATGTCACTTTACAAATAGGTGGAAGTGATCAGTGGGCTAATATTTTGGCTGGTGCTAACTTAATTAAAAGAAAAAATAATGCTAGTGTTTTTGGATTAACTGCTAATTTATTAGTTAAAGCTAACGGAGAAAAAATGGGTAAAACTAGTAGCGGAGCATTATGACTTGATGAAAATAAAACTAGTGTTTTTGATTTTTATCAATACTGGATTAACCTTGATGATCAAAGCTTAAAAAAGACTTTTTTAATGCTAACAATGCTTGATAAAAAAGTAATAGATGAATTGTGTAATTTAAAAGGCCCAAAAATTAAACAAACCAAGCAAATGCTAGCCTTTTTAATTACTGAATTAATCCATGGCACTAAAAAAGCAAAAGAAGCACAACAACGATCTGAACTAATATTTAGTAATCAACCAGATCTTGATATTAAGTTAGTAAAAACAAGCACTAATCTAATTGATTATTTAGTTGAAACTAAATTTATTAAAAGTAAATCAGAAGCAAGAAGATTAATTAGTCAAAAAGGTTTGACAATTAACAATAAACACGTTTTAGACTTAAACCAAATAATTGAATGAAAAGAAGAGTTACAAATTATTAGAAAAGGTAAAAAAAGTTTTTTAACAATTAAAACTGTTAATTCTTAGCTAATTTATCTTTAACTTCTTTTTCTATTTCAGTTTCAACACTTTTTTGACTAGAGCTTTTACTTTCTGGTTTTTTGATTAATTTGTTATGAAGTAAATCAACAACTTTCTGCATTGAATTTAATGTTTTGAAATCATAGCTTGCTTGGAGTTTAATGTTTGTAACTAGATCATCAAATGCTTCATTCACTTTTAGTTTAGTATCCATAAAGGTTGTAGGCACACTCATAAACAAGTAAGTATGAACTGTGTCATTATAGACATTAAATTTGGCATATACATTCAAAGGATTAAAAAGCACTTTATTTAATACCTCTGTATCTAAATTCTGAAAAGAAAAATTTGAATCTTTTTCAACAAAAAGTGAGATGTTTTCTTGCTTTTTGTTTTCAGTAAGTGCTTCAAACTGAGCCACATTCTTATTTAAAGAATAGTTAGCATCAGTTAAAAAGAAATGATTTTCACTATTAATTGCTGGTGTAATAGCAATCAAGCAAGCTACTTTTTCTGTTTTATTACTGCTTCTTTTAATTGCATAAATATGACCAAAAACAAATTCACTACCATCTTTATAAGTAACTTTAAATTGTCTTGGAAGTGGTTCAAAATTGTAAAGAGCAGTTGGATAAAAACTTGGTAATATATCGCTGCTTATAGGACCTTGTTCAAAAAATTCAAATGATTCTATTGCCTTGAAATTACCCATCTCTTTTTGTCATAAATTAGTTCTGTTTTCTTTGTTAACTTTTTTTAATAGCTTGTTTCTTTTAAAAAGAGCAGCAAAATGGATAACAAATGCTAAAAACAAAACAACACTACCTAAAACAATTAAAAAAAGTGATGCTATATTTGGTATTTCATTACGTGTATAACTATAAATACCAAATGAGATAGCAATAACACTAAAAAGACCACAAAGTCCAATTACTCTTAGAAAAGCATTGAGTTTATTTGGTGTCAAAAAGAACTTGTTAAATTTAGTTAGATCTGACATATTTGTATAAAAGATTGTATGAAAATATAAACCAAAGCAAGCTCAACAGTTGTTTTATTACAAGATCTTTTTCCTAAAAACTGCAAGTTGCCAAACTTAGCACGCATCTTTATTAAACGAAGATAGAGCTCATCAAATTTTATATCAAGTATTTTGTATCTTTTGTTTGTTTTGGAAATAAAAGTGCAAAAGTACTTTAGAAAATAAAAAATCAGATCCCAAATTCGTTTTTGTCCTAAACTAATAGTTTTGTTTTTTAGTAATACTTCAGTTTGCTTTAAAACATTTTTAAAAGTAATAACAAGATCATCATTACTGTCATTTAAATAAGTTTGTATGTTTTCAATTCAAATCAAAATGTAGTGATATTCACTGTCAAACAATTGGGGATTATTTTTAATTACTGTAAAAAAATCAGTAATTGTTTTACTTTGATCATTTAGTACTTTAAAACAATTCCAAGCTTCATTACCATAACCAACTTCTATTGCATTTAATTCTTGCTTAAATGATAGTAAGTTAACCAAGAAGTTATCAATAAAATCAACAACACTAACTGTTTGTTTCACTTTCTGGTTTTACTTCAGATTTATTTAGTTTTTGCTTTGCTTGTTGTTCTTGCTTTTGCAATAAGATTTCTGGTGGTAGTTTAGTGTTTTTATGGATGAAGTCAATATCACTTTTCAAAATAGTTTCAGCAATTAAAAGTGCTTCTACAAGCAATTCTAGTTCCTTACGGTTACTCTTAATGATTGTTTTTGCTTTCTTATACTGTTCTTCAATAATGAAATTAATCTCATTGTCAATATCTTTAGCAGTTTGTTCTGAATAAAGTTTTACATTAGAAGGGAGTGTCCCTTGACTTGGTACATATTGCACTTGACCTAATTTAGACATCCCAAGCTGGGTTACCATTGCTCTTGCAATATTAGTTGCTTTATAAAAATCGCTAGAAGCGCCAGTAGTAATTTCTAAATTACCATAGATTTCCTCTTCAGCAGCTCTACCGCCCATAGCAGTTGCTATCATTGCAAGTAAATCAGATTTTCTTTTTAGGTTAAGATCACCACTCTTAGGTGTTGAAAGTGTGTAACCCCCTGCTTGACCACGAGGAATAATGGTAATCTTTTGTACTTCATCATTACTGTGGACATGTAAACCAACCAAGGCATGACCAGCCTCATGATAAGCAACTAGTTTTCTATCTTCATCACTAATTACACGTGACTTTTTAGCAGGACCAGCTATTACTCTATCAATTGCTTCATCAATGTCATTAATGTTAATTGTGGTACGGTTGTCTCTAACTGCTAACAATGTAGCTTCATTGATAACATTTTCTAATTGAGCACCTGAAAACCCAGGAGTTCTCTTAGCAACATCTAAAAGACTTATCTTAGAAGAGAGATTTTTATTTTCAGCATGAACTTTTAAAATCCCTTCCCTTTCTTTAATATCAGGGAGATTGATTTGAATATGTCTATCAAATCTTCCAGGTCTTAATAATGCATCATCTAATACATCTAACCTATTTGTAGCTGCCATTACAACAACACCTGTTCTGCTTGTAAATCCATCCATTTCAGCTAACAATTGGTTTAAGGTTTGCTCAACAACAGAATAAGAAGAGAGTTCAACTCTACCCCGTTTAGAACCAACTGAATCAATTTCATCAATAAAAATAATACAAGGAGCAGCCTTTTTAGCTTTATTGAAAAGATCTCTAACTCGTTTAGCACCAACACCAACAAGCATATCTTCAAATCCAGAACCCGTTGATTGAAAGAAAGGAACACCAGCTTCACCAGCTACTGCTTTAGCTAATAATGTTTTACCTGTCCCAGGTGGACCGTATAAAATTACCCCACGTGGGGATCTTGCTCCCATCTGGGCATATTTCAATGGATTTTTTAAATAATCAACTATCTCAAGCAACTCATGCTTTTCCTCTTGAAGTCCAGCAATATTGGTAAATTTCACAGTTGACTTAGCCAACTTAGCTTGGGTTTTGCCAATAGAAAAAATATTATCTTCTTCTCTGCCCCCTGCAGATATACCCCTAGCACTTCTTCAAAAGAGTAAAAAGAAAACTACAAAGATAATAATTGGTAGCAAGCCAAACAAACCATTTAATACATCTCTAGCTCTAGTATCAGGAGCAATGAAAGTACCAAGGGTTTCAAAACCTGCAATACTCTTTGTGTTATTACTAGAACCACCATTCATCATGGTTCCATTACTATTAACGGTAATCTGACCATTACTTTGGTTAATTGATAAGTTAGCAATATTAAAGATAACATTGCCATTACTATTAACAGTTTTGTGAGCAGTAACAGTTAATGGACTATTTAAACCATCAAAGGTAATGGAAACTTGGAGAATGGTATCAGTAACATAAGTTGAACCATTTATTTGTTTAAATGTCAGTTCATTACTAAAACCGCTTACTTTTGCTGTTAAAGTGCTGTTACTACCTCCATTTAATTTTCAGCTTTCAACTACTGCAGTAGCAGCTCTTGGACTGAAAATATAAGCTAAAACACCAATAACAACAGCTAAAATGATGACTCATCAAAAGACTTTTCAAGCAGTTTTACGTGAAAAATTATTTTTTTCAGTAGTTGTTTGTTCTACTAAACCCTTATTTCTTTTTTTCATCTATGTTAGTTTTTGACCGATTTGGGTTTTTTTGGTTTGAACTACATTAACTGTAATTATACTGCCAATGGTAATGAAACTAATAAGTGTTAATAAAGTGACGAAAATTATCTTTTTATGTTTACTTAAAAGTGCAATAAAAGCCTTGAACCAACGCATAAAATTTTAATATCTGTTTTAGGGATTGTCTGGTTCAAACACACCAACATAAGGTAGGTTACGATAAAAACCATCATAGTCAAGACCAAAGCCAACCAAAAAATTATCTTTTACTTTAAAACAAGAAAAATCAATATTAATATCAAAGGCTTTGGGTTTAATCTTTTCAATTAAGCTAATTAAAGTTATTGATTTAGCATGCCTTGTTTTTAGAAGATCAATAACTAATTTAATAGATCTACCACTATCAACAATATCTTCTATTAAAAGGATGTCTTTATCTTTAGGGTCATGGGACATATCAAGCACAATCTTAGGTGGTTGTTTTTGTACATGTGAACCATGATAAGAAGCAACTGCCATAAAATCTAGTTGGAGGTCAAAACTAAATTTACTTATCACTTTGCCAAGGAAAGGGATGCAACCTTTTAGAATGCCAAGAACAATTACCTTTTTATTATTAAATTTAGCATTGCACCAATTAACTGCTTTTTGACAGCCTTCTTCTATCTGTTGTTCATTAATAACAATAGATTTAATACCCATCTTATTTAGTTTTTTGACGTTCTACTAAAACAACAGCTAAGCAATTAATTTCATTACTGTCTAAAGGTTTTCTAACTTTGAGATTAATTGTTAATTCTGAAATTCTTAAAGCACTACAAATAAAAGCAAAGATAGCGTGTTTATAATCATCAACCTTTTGCTCTTGAGGAATCTCTAAGGAGATATCAATATTATTGATAATTCAGTTTTTTCTGATGAAATTGTAAGTTCTTGCTAAGACTCTTGGTGCTTCTTTTTTTGCAGTACCTTTAAATTGGACTGTTGATTTTCCTTTATTGAAAACAATATGACCATCACCTAATGCAGTTGCACCAAATAAAGCATCAGCAACTGCTAATTGGATAACATCACTAACTTCATCACTGTTTTCAAGATCATAAATATATTTAGAATTCTCAAAATCAACACCGCCTAACCAAAATTTATTTTTACTTTCTTTCCTTAATTTTATGTGGTATTTTTTGCTTCCTAGGCCAACTCTTAACTGCATTGTTACAAATAATTATAATATCGCTCATCTTTAAATAACAGTGTTGTTTTGTAATCCAAAAAAAGTAAATTCAGGCAACAGTTTTTTGACTTCAAATATCCTTATTTAGACAAAGCAAAATTTATTAAAGTGAACCAAAATAAAATTGAAGTATATCTCTAGATAAATAAATATGAAAGGACCTAAAATTGCAATAGTAGGATCAGGTGCTGTTGGTACTAGTTTTCTATATGCTGCAATGACTCGTGCACTTGGTAGTGAATACATGATTATAGATATCAATGAAAAAGCTAAAGTTGGTAATGTATTTGATCTTCAAGATGCTTCCTCATCATGCCCAAATTTTGGCAAAGTAGTTGCTGGAGAATACAGTCAATTAAAAGATTATGATTTTATTTTCATTAGTGCAGGAAGACCTCAAAAACAAGGTGGGGAAACTAGGTTGCAGCTACTTGAAGGCAATGTAGAAATTATGAAAAGCATTGCCAAAGAAATCAAAAAATCTGGTTTTAATGGAGTTACATTAATCGCTTCAAACCCAGTTGATATCATGTCATATACATATCTTAAAGTTACTGGATTTGAACCTAATAAAGTAATTGGCTCTGGTACTTTACTTGATAGTGCAAGATTAAGATATGCAATTGCAACTAAGTACCAAATGAGTTCAAAGGATGTACAAGCATATGTTATTGGTGAACATGGTGATAGTTCAGTAAGTATTATTTCATCAGCTAAAATTGCAGGACTATCACTAAAACATTTTTCTAAAGCTAGTGATATTGAAAAAGAATTTGGTGAAATTGACCAATTTATCAGACGCAGAGCTTATGAAATTATTGAACGCAAAGGTGCTACTTTCTATGGAATTGGTGAAGCTAGTGCTGATGTAGCTGAACAAATTTTGAAAGATACTAAAGAAGTTAGAGTAGTGGCTCCTTTACTTACTGGTCAGTATGGAGCGAAGGATATGATGTTTGGAACTCCTTGTGTACTTTCAAGAAAAGGTATTGAAAAGATCTTGGAAATAGAACTTTCAAATACTGAAAAAGTTGCGCTTGAAAATTCAATTAAAGTTTTGAAAGACAACATTAAACTAGCAAAGCTTTAGTTTTGATAGAAAACATTAGCTCATCTATAAAAAGAGTTTTCAAAAAATAACTCGTTACTTCTCTTAACAAGCATACTAATTTCACTGAGATTTTTAATCTTGTTTTGAGAAGTTTTTATTTTTAATTCATTTTTGTGCTTATTTCATCCTTCATAAAAACCCTTAAATTGGTTATTGAATTTAGTAATGAAATAACTAGGAGTTTGATATTCTTTTACTTTTAATTCAAAATCAAGATCACGTTTTTCACAACTTTCATAAAACTTTACTTGAAATTTCAGAGTGTTAAGATAACTATCTAGTAAGGTTTTTAATATTGCATCTTTAGTTGTTTCAAATGTATTAACTAAAAATGAGTGAAAAACATAATCATCTAACTTCACAAAACAAGTTAATAAACTTTTTTTAAACAACTGGTTTTTTAAAAGCGGTTTAATTACACTTTCAACATTCTTAAAGTTATGAAAATTAAAGTTGTCTTCTTCAATTAAAGCTTTAATCCGTTGAAAAACAAATCATAAGTTAAGTTCTGTAGCAATACTTCTACCATCACTGTAGATTGATTGATACATGTGGTGTCTTGTTATCAAGAAGTTTTCAATGAAAGGCAAGCATTTTTCTTGAAAAACAATTTCATAAGTGCCCTGATTATCTACACATTCCATTCCATTGATAATTGATTGATAATCAACTAATGAGTGACTGGTACCTGTAAAATAAGCATCACGTAATAAATAATCCATTCTGTCTGTGTCAAGATCTGAAGAGATTAGCTGTCTCATCCACCAGTTAATAGGTTGAATATTTTGATTTTCATCAATTAAAGCGCCAATCAGATTTGGATCTATCTTATTAGCTTTTAAGATAGAGACAATAGGTTCACTATTAACTAACATTGAAGTAACCTTTTCATGAATAAAAAGTTGTTTTTTAAAATCAGGATTTTTAGCAAAATAGATTTCAAAAGCATGAGAATGAGGTCCATGACCAAGATCATGCAACAAACCAGCTACTAAAACAGTTTGTTTTTTAATTTGATCAATATTTAAAAATGCAGAACTATTTAAGATCCTTCTTATCAGTTCATAAACTCCTAAAGAATGGGCGTACCTAGTGTGAACTCCACTAGGATAAAAATGGAAGTTAATTCCTAATTGTTTGATGTTTCTTAACCTTTGAAAAGCTTTTGTATTGACCAATTCATACATCCACTTTGTGTTTTCATCAAAAATAATTTCTCCTAAAATAGGATCTTTAAAAAAGGTTTGTTGCATTAATAAATTGCTTAATTAAGTTTATTGCACTTTCTTTATCAAAAATTTCAATAATATGTGCTAAATCAGGTCCATGCTCCTTATTTGTAAAAATTAACCTTATTGGCATAAAAAGTTGTTTACCTTTTAAGTTAAACACTGCTCCTACTTTGTTAATAGTTGTTTTAATTTGCTCAGCTTTTCATTCTTCCAACCCTTGTAAAGATTTGGCAAGCTGTTCTAAAAAAAGTTTGATGTTTTTAAACAAAATATGACTTTTCTTAGCTAAGTTTTCAACACCTATTTTAGTGGCAAAAGATTCTCTTATCAATTCGTTTATTTGAACACCATGGGTTATTTGATTTTTAAAAAGTAAACTTATTTCCCTGTTTTTATCTTTTAAATAATCAACCTTAACATCCAAGTAATTATCAATGAAATTGAAATAAGCATTATCAGTTAATTGTTTAATGTAATTAGCATTGATTCATTGCAGCTTTTTAATATCAAAAAAAGCAGGAGCACTAACAACTCTACTTAAACTAAAGTTTTCAATTAACTGTTTCAAATTAAAAAACTCCTGGTTGTACTGTGGATGTCAACCTAAGAGTGCTAAGAAATTTAATAATGCTTCAGGTAGATAGCCTTGTTGTTTAAACTGCTCAATAAACTGAGTAGTTTTCTCATCACGTTTAGAAAGTTTTTTGCCACTTTCATCAACAATAACTGAAAGATGACCAAAGCGGGGAATTCTTTTAAAACCTAATGCTTGATATATAGCAAGTTGGTATGCAGTGTTAGAGATGTGCTCAGCTCCCCTTAAAACATCAGTAATTTCCATATCATAATCATCAATAACAACTGCAAAGTTATAAGTAGCAACACCATTAGCTTTAAGAATAACTATATCTGTTAACGCACTGCCGGGAATAGTAATTTGTCCCCTAACCAGATCATTTCAACTATATTCAGCTTCATTGTTTATTTTTAAGCGGATAGTAAAAGGATCATTTTTTTCTAAGTGATTAGTAATTTTCTTGGAATGTAAATTACGACAATGACCTAAGTATTTAGGGGTTTTGTGGTTATTAATGGCTTGTTTTCTATCTGACTCTAATTTTTCTTTGCTACAGAAGCAACGATATGCCAGATTTTTTTCAATTAAATCAAATGCTAGTTTTTTATAAACTGCTAGTTTTTGTGATTGCAGATATGGACCATAATTGCCAGGGTTATAAACCGATTCATCTGCAATGACTCCAAGCCAACGCAAGTTATCAAATTGTGAATTAATTCCTTCTTTTATGTTTCTTTCAGTATCAGTATCTTCTATCCTGATAATAAACTCACCATTAAAGTGCTTGGCTAGTAAAAAGTTAAAGATTGCTGTTCTTGTACCACCAACATGCAGATATCCTGTTGGGGATGGTGCATAACGTGTTCTAATTTTTTCCATTACAAATGTTTTGGTAAAGATTAAAGAGTTGACAAGGACTCAACTCTTGCGCTCTAATGCTAGTCTTTAAATTTTGCTTTTGAATAATGTTAAGTAAATAATCAACTGCAAAAAAATGCTTTAAGTTATTAATTAACATCTTCCTTCTTTGATTGAAACATTGCTTTAAAAACAGACCAAACTTAAAGTCATAACTAACCGATTTATTTTTTTCTAATAATATTAAGGTTGAATCCACCTTAGGTTTAGGTTTAAAAGCATGCCTATCAATTTTAAAAACTGTTGTAATAGTCAAATAGTATTGACAAAAAGCACCAAAGGCACTATAATCACTAGAATTAACCTTTGCCAGAAGCCGATTAGCAAACTCTTTTTGTGTCATTAAAACAAAGCTTCGAAGCTTTGATTCTAAAAACTTATTGATTATTGGAGATGTAATGCTATATGGGATATTACCACACAATAATGGACTTAAATTTTCAAAAAAACTATTAAAGTCCTTTTTGAGAATATCGCCTTTAACTAGTTGGTCTTCAGTTAATATCTTTTCAACTAGAAGATATTCAATTAAGCGTTTATCAATTTCTATCCCCTTGTAAGGTATTTTGAGTTTTAACAAATAATTTGTTAACGCTCCTTTACCAACACCTATTTCAACAATTGCTTGTGGATTTAAATTTTTAACAAAAGCAAAAATTCTTTTAATGACGCTTAAATTAACCGTAAAATTTTGACCTAATTTACGTGAAGGAAAAAAACTATTCACGCTCTAAAATCAAATCTAATTTGGAATAATTTTGCTTTCTTTTTTCCCTTCTTTTGACAATGAAAACATGGGTTAAATAAGATTGTAATAAAGTAAACAAAGCATTTAAAAACCAATAAACACCAACTCCAGCAGCACTAAATGCAGTAATAGCAGCAAACACAAAGTAAAAAATTAATTGCATCTTTTTAGTTTTGTTGAGTTGTTCAATACTTTTTTGAGAATGGGCTTTCGCATTCTCATTACGCTTACTTGCCCACACTTGAGGAAGTTTTTGAGAGAGAAATTGGACTGGTAAAACAATCACTAAAAAGATGATGAAAGGTCAACCAGTTGTAGTGAAATTAGAGAAAATTTCTGTTAAAGGTACTTTTGAAAGATCCCAAAAGTTAAATAAGATGATTGCTTTAATTGGTCTTAAAGTAGTAACAATCCTATAAATAATTAAAAAGATAGGTAAAGTTACAAAAACTTGAACAAAAGCAGCAGATGATTTAATGTTGTGTTTTTTATAAAGTGACATTATCTCTAACTGTCTGTTTCTTTTACTTTGTAAATCTATTGCCCCTTTATATTTAGCGTTAATTTCTGCTAATTTACCTTGCACTTCATTCATCTTTTCAAGTGCTAAAGTAGAGTTTAAGGTAATCACAATAGTTATCAAGCGAACTAATAGTAAAAGTACTATTAATGAAAGAATCATGTTAAACCCAAGTTCCACCCCGCTACCTAGTGGAACTCTGGTTGCATACATAATTGGTAAAACTATTTGCGCTGCAGGCCAGACAAATCATCCATAAAAAGGGCCATATGCTAAGGTGTAATCACTAAAGGTGAAATAAGGGCCAAAGTTATTTGAAATCAAATCATACCTATAATCACCAGTAGTACCAAATTTATAACCTATCTCAAGTCCTGAACCTAAGACTTGATTAGTACCTGTTCAGGGTTGTGCTAAGGTTTGAGTACAGCCCCAAAGCCCAACAATAGTTAAAAAAATAAAGATAAAAACCTTAATAACCTTTATGAAAATAGCTGAAAACTTTTTGAAGTTTTTTCAGTTGTTTTTTTCATTAACAACTGCTGCTGATCAAAAAGGATTAAAGGTGGTTTTTATCTCTTTATTTGTTTGCGCTAGTTTGATAGGCATCTACTTCTTTTATCCGCTTTAATAGTTGCAAAAAAAGTTTTTGTTTTTCTTTAAATGTTAATTCAATAAAGCCTTTGTTGACAATAACTAAAATATCTCAAGGTTCTAAATTATTACTAATTTGTTGAAAGATACTACGGATCTGACGCTTAATTAGGTTACGTTGTACTGCTAGCTTATATTTAGTTTTTGCAATTGATATTGCAACCCTTCAAGTAGAATGATTATTCTTAATAAAATAAGCGTTAATAAAGGTACCAAAGAACCTGGTTTTACTTTGAAGAATGGTTGTAAAAACCTTGCGTTCTCTTAAGCTGTGACTCTTTTTAACGCTCACTGGAAACCGTGAGTTGAGCACGATTTTTAAAACGTCTTTGCCTTAAAACTTTACGTCCTTGTGCAGTTGCCATCCTAGCCATAAAACCATGGGTTTTAGCACGCTTTAATTTGCTTGGTTGGTATGTTCGTTTCATGAAACAATTAATCAGGTGATTATAACTAAACTAAGGGTCTGACTAATTGGTTAGATACTATTTTACCATCTGAAATTTTAATGATTCTATTAGCTATCCTTGTTAGGCTTACATCATGGGTAACCATTAGGATGGTTTGCTTATATGTTTTGTTAATTGTTTGTAATAAATTAATGATATTTTCCGCAGAATTACTATCAAGTGCACCAGTAGGTTCATCACAAAGTAATAGTTTAGGTTCTTTAATAATAGCACGGGCGATGGCCACTCTTTGTTTTTGCCCACCTGACATCTCATAAGGAAATTTATACAAGATTTCTTTAATATCTAATTTTTCAGCCAACTCTTCTATATCAAGTCGTCTTTGATATTTGGGAATTAAGTTTTGTGAAATGGCAATATTATCATAAGCACTTAACAATTCAATCAGATTATAACGCTGATAGACATAACCAAGGTTATTCTTTCGATAAGTTAAAAGTTGTTCACTAGTACATTTTTCTAATGGACAGTTTGCTACGATACAACTCCCTAACGAGATAGAATCATAACCACCTATCAGGTTTAAAAGGGTTGTTTTACCTGATCCTGAAGAACCTAAAATGATTACTATTTCACCATAAGCTATCTGTAAAGAAACATCTGAAAGGATAGAAATCTCTTGCTTTGAATCATCATTATGAACATTTTTTCACATCTTATTGATGGTAATAACAATTCCCTTAGCTTGTTGAGCAAAAGGAGGGATATATAAGGTATTGGCTTTTTGCTTTTTATTAGCTTTCTTTTTGAGCTTTAGCTCAGCTTTTTTAATGTTATTTAAAACTTTTTTATTCTTCTTAGGAGGTTTAGATTTACTCTTTTTGAAATAATCATCGAAATTGGCACTAAAAGAAGGACTTTTAACGTTATCTAAGATATCAATACATTCATTTCAAACAGTTTTATTGCCTTTTTTCATTAACTAAAACCCTTCCTTCAGTTCAGCTGAGCGTAACTTAATTCAAAAGTAAGAACCAATTACAAAGATAGTAACACCAAATAATACTATCCCTACCATGTATGGCAAGAACTCAAATACATTAGTTAAAAAGACTTGTGCAACATTGAAAACAACCGCTTGGATACCAATTAAAACACCAGCTAAAACTCCAATAGAAATTAGTAATGCTAAAACAAATGCAGGGATAAAGAAGGTAAGTAAACTAATTAAATTTTGTACATCTCTATAGCCAATAGCCTTAAGTGAAATAAAGATTCTTTTCATCTCTTCTAACATTGAGATACCAAGTACAACACAAGTAATGAAAATGAAAGGAATAATAGTTCCTAAAAGAAACGCATCTACTTGATTCACAGTGTTAAACAAAACTTCAGCATTATTTCTAATGATCTGAGCACTAGTTCCAGCTGCATCAATATTGAGTACAGGAGCAATAATGTTATTACCAAAAATACTACTTGCATTTTGGGAAAGTAAATTAACACTATTTCATTCTGTTTTGTATTTATTAACTAGTTCTTTACGAAGCGATTCATAATTCATTGAAGTAGTATTAATAATCTGTTTTTCTTGTGCTAACTTATTAATAATTTCAGGATCAGTAGGTAGTATGGATGTAATAACTTTATCAAGTTTATTCCTTACTAAATTTTGGAAATTATTACCAAAGTCAGTGAAATTACCCCAAACACCAATTATCGAGTTTAATACTAATGCCTTATTTAATAAAGAAGGTTTTAATTCTTTGGAAAATACACCATTAAAAGGAACATAACCGTTTGGTTTTACATAATCAATTTCATATTCATCACTACTTTGAGATTGGCCATTTGTCTTTTTAATAACATCTCTAATAATACCTCTGCCCTGATTACCACTTAGTTTTAATAAGTTATTAGCAATGTGTTGGTTAATATAAAGTTCTTGTCCAAAAGCATCAGTAGAAACACCAACAACTTTAAATGCTACTGTTGAATTATTTAAGGGTGCTTTTAGAAATTTTTCAGAGTATCTTGTGGCAGTATTTTTCGGTTTTATATAAACATAATCACCAATATTAATGTTGTTAGCATATTGAAAACCAGCATTAACAATTACATTCATTTCCTGATCAGAACTAATTAAATTTCTTAAAGAGTTACCACTACCATCTATCAGATTAACAAAACTTGATTCAGGGTTAATCCCTGTTAAAGTGTATGGTGAATTCTCATCAAGATTATCTTCTTGAAAACCAAATGGGCCTTCAACATAAGTGTAGGTTTCTTCTATGGCAGGGTCAACAGGAACAATCCCAAAATTTAACTTAGAATCTTGTTTTGCAAGTTCAAAATCCCCATAAACTTTCAACATAAAACTAAGAAAGCTATCATTAAATTTAACCCCACCAGGATTAAATACATCAATTGTAGTTAGTACCCTACTAGCATCTAACTGAATTTGATTAGTTCTAGGGTTTCTAATTAAAAACCCATTAGCATTTAAGTTATTGAAAAAGTCATCAGTTAATACTGAAATTAAAAACTGATCACTAGATGCTGAAAGTAACTGAGTAATCTGTTTAGGAATTGATTTTTCAACAATTTCCCAAGGGTTAATGTTAGAACCGCCAACCCTAATAGGAAAGTCAATAATCCATTTTGATAGAGAGGCATGCCTAAAGAAATTCTCATCAGTGTTTAACTGTTGAGCACCACCATAACTAGGCAAAAGTAAGTTGCCCAATTCAAGCTCAGTTCCATCTGCTGCTTTTATTGGTTGATCCTGGCGGTTTTTAAGTTTTAAATTACTTGGTTTATAAGGGTGCTCATTTTGAGAATTTGCTTGAACACTTTCGTTATACAAGGTTTTAATACCATTATTATTATCAGTAACTCCAAAGTGTGAATAGGGTTGAATTGCATACCATCCAGATTGCTCTGTTGGTGTTTGTAAATTCAACTTATAGTTAAAGTTTCTATTTAAGGAAGTGCTTGTTTGTGCTGCACTAAATTTTTGGGGAATAGTACCTGCAATCCCAATTAATAAAAGTGCAACTGAACTCAATCCTACATAGGTAAATAAACGTGAAAAACGTGAAACGATTAATGAAACCCTAAACTTGGTCAAGGGTGACATTGTATGACTTTTATGTTTTAAAAGATGAAGAAGAACTGAAAACTTGGTCTCATTGCCTTGATCAATTAAGACATTAACAGGTTTACTAAACAACTGTTTAAAGGCAATTCAACTAATAAATTCAAAAACAAAAAAGGTTATAAAGAAAGAACCAAAAAACGATAACCAGTTAAAGCTATTTTCAGGTAAAGCAATAAACCAATACCTATTTATGATTCCCTTTACTTGTCCTTCTAGAAGAAAGGCAAATAAAACTCCTAAAAAACTAGAAACAATTGCAACAATACCAGCAAACACACTCATTCCAACAATAAATTTAGTTGTTGAAAATCCCCCTGCTCTGATAATAGAAAACTCAGTTTGATTCTTTTTAATAAATGACTTAATTAAAAGAATAATTAAATAAAGAGCAAGAATAATCAAAAATATAGCAATAACAGTACTTACTATTGCCAAATAGCTTTGCACTAAATCAGGAAAATAGTTTCTTGCTGTTGTCAGATAATTAAACTTAGAAATATCTTTAACATTAAATGCTAAATCACTTCTAGGAACATCTAGATATCCCTCTAATCTATTAGCAATAAACTGCACTGGGTCTTGGTCAGTGTGCTGTTTTAAATCTGTTGACTTAAAAGCATAATAATTTTCTTGGTTAGCTGCTGGTACAGCAAACAGAATACTTCTGTAAGCTTGATCATTAACAAAGATTAACGCTTCATCTTGGGTATTAGGCAACGGAGATTGTAAAGATAACACTGGGTAACCAGTTTCTACTGATTCACCTATACCTAAAATTGCAAATGTTAGTCCGTTAATACTAAATTTATAACGATCAGGTAGATCATTTAACCAGTTAGGGAAATTGTTTGGTGTTTGCAATGCAGTTTTTCAAGCATATTCCCCTTCCTGGGGAAGAATTTCTTTATTGTGTCTAAACAACCATTGTTGAGAAACAACCGCTCCGTGTGCTTCAGGGGTTTGCACAACTAAATTTTGTTGATCAGTAGGTAATATTAATGGATTGACCCCATTTTTAACAGTAATAGGGATCTCAAGCATTTGGCGATTTGTCTCTATTAATTCATTAGGATCTTGAAAAGCAGGAGTAAAATAACTTAAAAGTGCAAGATAATTATTAGCTGCTTTTCGTTTATCTTCTTCCTTAAGATTATTAAATCCATTGGCATCTAAATTAGTTCATTGTTTTAAAGCATCTAAAGCAGTAGTTGGGATATTAGTAGCTTCTGGTAATTGGGCTTGAGCAATAATATTTGCTAACTTTGGATAAATGGGAAATTTAGTAGATTTTGGACTTAAAGCTTGGGTATTTTTTAAACTAGCAGTCATTCCACCACTACTGGTGCTTGGTTTAAATTCATAATCAATTTTTTGTAAAAATCCATTATTAGCATCTTGACCACCATAAATTGTGTCTAAAGGGTTTGATTCATTTTGCTCAGGTTTAAATAACCAAGATTTAAGATCAACGCCATCAATTAAAGAAAGATCGTTGTATCTCTGTTCATCTTTGTTGGAAACTAAAAAACTTGCTGACTCTGCTGAACTAACATTGTTAGCTATTGCGATATTTTGCTTTGAATAATCAATGTTATTGAAAGCAGTGGCTTTTAAAAAATGATCACGGTACTTTTCTAAAAGATCAACTGTTTTTTTAACAAGATCCTCTTGAAAGATACTACGTCATTGATCAAGTACTGCTGCATAAACTGCATCAAGGGTTTTTTCAGGAGTGTTATTGGACTGGTGAATTTTATTGTATGCAGTTTGAACATTTGAAAAGTTAGGATCAGCTATTCCATTACTTGCTTGACTGTTTTGATTTTGAACTAATTGGAGATTGTTGCTTGAAGGTTGAAAAGAAAGATTATTATTTCTTACCATCATTCCATTCTGAGTTTTAAAGATTGTGTCTTTATCACTATCACTAAAACCTGGTAAGGAATTGAGAATTGTATCTTTCTCAGATTCAACTTTCTTTTTAAGTCAATCTTTATTAATTTTTAGTTCAAATTTCTTAAGTTGCTTAAGTTTATTTTCAAGATCTCAATCTCCAAAAGAGGAATTATTGGCTATAGTTTTAGCATTAGTTTCTAACCACTTTAACTGTTCATTATCTAGCTTATTTAATTTAGAAATATCTAAATTAAAAGTTAATGTAAAACTTTGATTATCTTTACTTTCTTTTTGGGTTTGAAATTCTTCAACAATTTCTTTAAAAGGATTGACATCTTTCCAAAATTGAGAACCAGGATCGATTTCTAAATCAATTGGGATGTTTGCATAAAGATTGGGAAATCAGTTAGCAGCTTCATTACCACCTGTAAATAGATAGTTAGCAGATATATCAGTATTATCTGCTTTGGTCTCATCACTCTTAAACCAAACTTTATCTTTGTTATTGGTTTTATAAAGTGAATTAACAGTACTGTCTTTTTGTGGATCATTGTTTGTTTGTTGCTTTTGTGTAATTTTTACTTGTGTTCCTGTGCTTTTTCCTTTATCACCAGGAAAACGCGCAATGCCAGCACGCAATGAGATAGTTTCTTTAATTAAACCATCAGCAAAATAACCTAGAAGGTTACCACTAGCATCATAAATAAGGTTTTGAGGGTCTGATATTATGTGTCTATTTGTAATGGAATCACTAATATCACTACCATTAAAAGAATCAGTATTATTAAAAGAAACTGCTAACCCTTTATAAATTCTTTTTTCAGCAATATTGGCTTCTCCATATGCTTTTAAAATACCTCTTTTTCTTGCTAAAAAGAGTTTATCTTTTTCATTAACATCGCCTTTGCGGGGAATGGTTTTGTTTTGGTTATCACCACTACCATTACTTTGTCCTGAATCACTTCCAAAATCATTTTCTTGATAAGGAAAAAGATAGTTAATTTTTTTATCATCTATCTTTATTTTGGAAAAATAACCACTATCATTAGTTAAACTGGCTATATAAACAGGATTAGCTTCCTTTAAAATAGCAGGAGCTACTATATCAGAAGAGTTAGTTTTAGATACTAATGTAGTGTAGGTACTAACTAAGTTGTTACTTAACTGAACAATAGATGTTTCAAGGAAAATAATGCTAAATAAAACAAAGATAATTCCAAATAATAAAAAGAAGAACTTTTTAAGCGATTTAAATATCTGTTTAAAAAAAGAAAACATCTCAATTAAACTCAGTTAATTTGTTCGATGGTTTTTGGATTCTGATTTAAATAATCAACGATTATCTTGCCATCATGGATTTTAATTACCCGTTTAGCTAATTCCACTATCTTTTCGTTGTGGGTAACTATTACTATAGTAGTACCTTTGTCACGATTATATTCCACAAAAAATTGCAATATTTTTTTGGAAATATCAATATTAACTGCTCCAGTAGGTTCATCACCAAACAAAATTTTAGGTTCTTTAATTAAAGCCCGAGCAATAGCAACACGTTGTTGTTGTCCACCAGAGAGTTTATGAACTTTCTTATGTCTATGTTCTTTTAATTCCAAGCGTTCTAATAATTCTTCAAGATTATTATTAAACCTTTTTTTTAATGGTAGAGCAAGCTTAATATTATCATCAACGTCAAGATCACGCAATAAACCATATTGTTGAAAAATATAACCAACGTTTTTATTTCTTAACGCGGTTAGTTTTGCATCACTACAACATATGGTGTTAGTTCCACAAACAAAACTATCGCCGCTAGTTGGTCGATCTAATGCGGAAATTAAAGAAAGTAAGCTGGTTTTACCACTACCAGATTTACCAAGAATAACAACAAATTCTCCTGGAAGAATTTTTAAATTAATGTGGTTACAAATAAGTTGGTTGTTAATGCCATTTGTAACAGCTTTACAAACTTCTTTGAAGTAAATATCATACTTTTTAGCTTCAAATGAATTAAGTGATTTTCTTTTGGGGTGCTTTTTATTTAACTTTTTAATTAATTTAGATGCTTTTTTACTAACAGCAAAATCTGATGATTTTAAGTAAATATCAAACTTTTTGTTCTCTTTTGTTTTTAAAACCATAAACAATCATTAAGTCATCTAAAAAAATACCCTGAGAACACTATTAGAAAATTTTAATTTTGCTAAATTACTAATGATGTAATTACATCCCGTAATGAACTGTCATTTTCAATCATTTTAGCAACTCTTTTAACTGCCATTAATACACTTGAATGACTTCTCTTAAATATTTTGCCAATTTGTTGAAATTGCATGTTGTACTTTTGACGCAAAAGGTAATTACACACATCACGAACACGGACAAGTTCTGCTTTACGATTTTCTGAAAGTACACTGTCCATAGGAACATTAAATCTACGGCAAACGTTCTCTATTAATAAATAAGGATCAAAGCTCTTTTTATGAAACTTCTCAAATTCTTCAAAAAGAATTTCTTTTAAATTTTCAGTATTTATTAAATTTTGTTTTGAAGTTTTAGCAAAAAATAATAACTTTGTTGCAATTCCATTTAAAGCACGCACATCGTTACCTGAAATTTGTGCTGCATCATGTCTTGCCTCATTAGTTATTTGGATGTTAGGATCTTTTTCTTTTAATTTAACAGTAAGTATTTCACAAAGTGAAGACAAATTATGCTTTTCTATCTTTAGTAATAATCCTGATTTAAAGCGAGAAATCATTCTTGCATCAATATCAATTAGTTCATCAGGAGCCTTATCAGAAACTAAAACAATTTGTTTTTTATTTAGAACTAGGTTATTAAAAATATTGAAAAGAATTTCTAAGGTTTTTTCTTTTCTGCCAAATATTTGAGTGTCATCTATTAAAACTAAATCTAAATTTTCATAATTTTTTTTTAGTTTTTCTATACCTTTATCCCTTTGATAAAAAGCATCAACAACTTCTTGGGCAAAATCACTTGAAACAACATACTTTACTCTGGCATTTGGAAAATTACGAAATTTTTCATTTCCTATTGCTTGTAGTAGGTGAGTTTTACCAAGACCGGTTTCTCCGTAAATAAAAAGCGGTGAAAATTCGTTATCTTGAGTTTCAGCTAATCTAACGCCTGCTTCATAAGCTCTTTTATTTCCTTCACTAATTACAAAGTTTTGAAAAGTATAGTTTTTACTCAATCCAGAATTTTGATAAAGAGTATCACGACTATTTTCTTCTAATTTTGCTAAGTTAAAAAAGAAATCTTGCTCATTAACAAAATTAACACTTTTAATTCCTTCATACAAACTTTCAGCTAAATGAATAATTTCAGAATTATCATTTAAGGAATTACGAGCAAATTCATTTTCTAAAAGAATTAAAAGAACATTATTTTTAAATACGAAACGATTAATGTCTTTAATGTATTTATCATGAAAACCTATTGTTTTTTCATAATGCTTTTTTAATAGAGATTTAAAGGCATTAAATTGTTCCATTATTCTTCTATAACATTGTCAAGAATGATAGTTAAAATTCTCGAAATTGGGATATTAACTGCTTTGGAGTAATTTCTAACTTTTTGTCATACTCTTTGACTTGTATAGAAGTGTACACCTGTATCTAGTTTTTCTTGGCGTTCAACAGGAACTATTCCTGGTATTTTTGTTTTAGGTTGGGGAGGAATAGGCTGTGGTTGTGTGAATTGTTGTTGAAAATTTTGATTTTTTTGCTGTAAGAAACCATTATTATGATATTGAAAATTTTGTTCCTCTTGAAAATATCTCTCTTTTTTTGGTTTTCCAGAAAAATTTGATGAAAAAGATTTTTCTTCATTTCAATTTTCAAGATTATTTTCATTTTGTTGATTTATTTGCTCAGGCTGTTGAAATGAATTATTTTTTGATCAAAAAGATTTTGGAAAGGTTTTTTCAAAAGCAGATAAAGGTCCAAAATCAAATGAAGATGAATCTTTGTCAAAAGATGTTTCTTCTCTTTTTGACAAATTTTGTTTTTGATTAAACTTATTTTTATTTTGGGGTGTTACTTTTTCTTTTATGGAAAACAAATCTTCTTCTAAAAGACTTTGTTCTGGGTCATCATCTTGTGCTAAATCAAAGAAAAAACGTTTCTTTTTGTTATTAATGGACATTGTAATTTGCTAAATTTAGGATTTCTTTTGTTATTTCTAAATACTCATTTAGATATTTTTTACTTGGTGATGATACTAATGATATTGGCAATTTTTCATAACCTACAGCTGCTGATGATTTTGATGTCAGAGAAACAAAATTTTTAGAAAAAGCTACATTATTTTTTTTAGCTTTTGTTTTAGCTAAATCTATTACTTCATTATGAAGACGAGTACGAACGTTAACTTTTGTAGGAACTAAAATAGTTTTAAGATTTGTATTTTTTTCCTTAAATGTATCTATTGTTTCAACTATTCTCATCAAACCTAGCATCGAATATTGATCTGGTTCAAAGGGAATAACTATGACATCTGATAAACTCATTGCAGTAGAAACTAAAGTTGCCATATTTGGTGGTGTATCTAATAAAACAAATTCATATCTTTTTGCTAGTTGCTTAACTATTTCTGCTATATCTGAGGCCTTATATTTTTTACGTGATATGTCTATATCAGCAAAATTAAGTTCAAAATTACAAGGAAGAATATCAAGTCCCTCATATACAGATAGCAAGCAATCATCTATTTCAATGAAATTATTTGAACCACTGAATTTTGGAACCTTCAACAAAATGTCAATTAACGTGTTATTCAATCTTTCAGGGTTTTGTCCAAATGATGCAGAAACATTCCCCTGCCCGTCAAGATCAAGAATGACTTTTCGCCTTTCTGGACAAAGTTTAACCAATGATCCTGCAACATTAGTTGCCATTGTAGTTTTTAATACGCCGCCTTTATTATTTACAAAAGAAATGATCATATATTTAAATGATTATAATATTTCTTTAATACTAAAAAAATAC", "length": 580076, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_000027325.1|L43967.2", "orig_description": "HMT-616 Mycoplasmoides genitalium G-37" } ], "run": { "start": "2026-01-26 07:22:59", "end": "2026-01-26 07:31:19", "duration": "8.34 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }