{ "genome": { "genus": "Mycoplasmoides", "species": "pneumoniae", "strain": "FH", "taxon": "Mycoplasmoides pneumoniae FH", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 811088, "gc": 0.40003674077288776, "n_ratio": 0.0, "n50": 811088, "n90": 811088, "coding_ratio": 0.7963303611938531 }, "features": [ { "type": "oriC", "sequence": "contig_1", "start": 29, "stop": 690, "strand": "?", "product": "origin of replication", "nt": "GGTATTAAGATGCTACCAACGTGGTATTAAAATGTGCCCAACCGCGAAAAAGAAAGTGGTATATAGGAAAATGGAAAACAAATTTGTTTACCGTTTACTATTAAATACTAATCTTCTATATAGTATAGAGAAACTTTTTCTTTAACATAATATTATCTTAATATTATTTACCTACTAATAGCTTAATATTATTAGTATTTATTTAGTATTATGCTAATACTATGCAGATATTATCTTAATATTATCTATAGTATTAGGCTAATATTATTCTTAATATTTATTAAGGTACTAAAGCATTACCTATAGGTGATATTATGACAATACTAAAGTGGTTAGTATTATTAGGGTATTATTCAAAGTATTCTCCAACACTATTCCCTTAGCTCTAACTTAATATCCCTTTAATACCAAGATGGTTAAACAATGAAGAAAATTAGTTGTTTGTTCTGGCCCTTTACTATATATGTACATAGTACATATATAAGAAAGGCTACCATGACATTATATATAATAGGCATCCAGAAATAAAAGCCAAATTAATCTATAATTAAGTATTTATACTTAATTATAGTGATTTTATTAACAAAATTAGTGACATTTGGCCATCCATAATAAATAAAATCAAATAAACAAAACAAAAAGAAAAAAACTATAAAAAGGGC", "id": "CNOCLNGIAL_1433" }, { "type": "cds", "sequence": "contig_1", "start": 691, "stop": 1833, "strand": "+", "frame": 1, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360", "KEGG:K02338", "RefSeq:WP_014325278.1", "SO:0001217", "UniParc:UPI0001DD643E", "UniRef:UniRef100_A0AAX0SSD5", "UniRef:UniRef50_P47247", "UniRef:UniRef90_Q50313" ], "nt": "ATGAAAGTTTTGATTAATAAGAATGAGTTGAACAAAATCCTCAAAAAACTCAACAATGTAATCGTATCTAACAATAAGATGAAACCATACCACTCTTATTTATTAATAGAGGCTACAGAAAAGGAAATTAACTTCTATGCTAACAACGAGTACTTTTCTGCTAAATGTACCTTAGCCGAAAACATTGATGTACTTGAAGAAGGTGAAGTAATTGTTAAAGGCAAAATCTTTAGCGAACTCATTAACGGAATTAAAGAAGACATCATTATTATTCAAGAGAAAGATCAAACTCTTTTAGTCAAAACAAAAAAAACAAACATTAACCTTAACACGATTGATAAGAAAGAATTCCCCAGAATCCGTTTCAACCAAAACGTTGATTTGAAGGAATTTGATGAACTTAAAATCCAACACAGCCTTTTAACTAAAGGACTTAAAAAGATTGCCCATGCTGTTTCTACATTTAGAGAATCCACTAGAAAATTCAACGGGGTTAACTTCAACGGTTCCAATGGTAAACAAATCTTTTTAGAGGCATCGGATTCTTATAAGCTCTCTGTTTATGAAATCAAACAAAAAACCGATCCATTTAATTTCATTGTCGAAACTAATCTTTTGAGCTTCATCAATTCTTTTAACCCTGAAGGTGGTGATTTAATCAGTATCTTCTTCCGCAAAGAACACAAGGATGATTTAAGTACCGAATTACTGATTAAGTTAGATAACTTCTTAATTAACTACACCTCAATTAACGAAAGCTTTCCGCGGGTAATGCAGTTGTTTGACTTTGAACCAGAAACCAAAGTAACCATTCAAAAAAACGAACTTAAAGATGCATTACAAAGAATCTTGACGTTAGCTCAAAACGAGCGCTTCTTTCTCTGTGACATGCAAGTAACCAACTCCCACCTCAAAATTAATTCCAACGTTCAAAACATTGGTGCATCCTTAGAAGAAGTTACTTGCCTTAAGTTCGAAGGTCACAAACTCAACATCGCTGTTAATGCGCTTTCCCTCTTGGAACACATTGACTCATTTGATACTGATGAAATTGAGCTTTACTTCCAGGGCAGTAACAAATACTTTTTAATTAGTTCGAACAACGAACCTGAACTTAAAGAAATCCTAGTTCCTTCCAAGTAA", "aa": "MKVLINKNELNKILKKLNNVIVSNNKMKPYHSYLLIEATEKEINFYANNEYFSAKCTLAENIDVLEEGEVIVKGKIFSELINGIKEDIIIIQEKDQTLLVKTKKTNINLNTIDKKEFPRIRFNQNVDLKEFDELKIQHSLLTKGLKKIAHAVSTFRESTRKFNGVNFNGSNGKQIFLEASDSYKLSVYEIKQKTDPFNFIVETNLLSFINSFNPEGGDLISIFFRKEHKDDLSTELLIKLDNFLINYTSINESFPRVMQLFDFEPETKVTIQKNELKDALQRILTLAQNERFFLCDMQVTNSHLKINSNVQNIGASLEEVTCLKFEGHKLNIAVNALSLLEHIDSFDTDEIELYFQGSNKYFLISSNNEPELKEILVPSK", "aa_hexdigest": "3a85738d3f3ec1acc1ab810cb8dc3a0f", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0001DD643E", "ncbi_nrp_id": "WP_014325278.1", "uniref100_id": "UniRef100_A0AAX0SSD5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD643E", "RefSeq:WP_014325278.1", "UniRef:UniRef100_A0AAX0SSD5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SSD5", "uniref90_id": "UniRef90_Q50313" }, "psc": { "uniref90_id": "UniRef90_Q50313", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47247", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_P47247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47247" ], "product": "Beta sliding clamp" }, "genes": [ "dnaN" ], "id": "CNOCLNGIAL_43", "locus": "GCA000143945_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1837, "stop": 2082, "strand": "+", "frame": 1, "gene": null, "product": "Chaperone protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8W2", "UniRef:UniRef90_A0AB38W8W2" ], "nt": "ATGACGCTTTATGACTTACTAGAGCTGCCCCAAACTGCTACACTACAGGAAATCAAAACAGCTTACAAACGCTTAGCTAAACGTTATCACCCTGACATCAATAAACAAGGGGCAGATACCTTTGTCAAGATTAATAACGCCTATGCCGTTTTAAGCGATACCACCCAAAAAGCGGAGTATGACGCTATGCTACGCTTTAGTGAATTTGAAGACCGAGTTAAACGCCTGGACTTCTCGATTAAGTGA", "aa": "MTLYDLLELPQTATLQEIKTAYKRLAKRYHPDINKQGADTFVKINNAYAVLSDTTQKAEYDAMLRFSEFEDRVKRLDFSIK", "aa_hexdigest": "ada1fc6b9036bcb5b7b7243b6261452f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1837, "stop": 2305, "observations": { "insertion": [ 2239 ], "mutation": [ 2080 ], "stop": [ 2080 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8W2", "query_cov": 1.0, "subject_cov": 0.5225806451612903, "identity": 1.0, "score": 161.0, "evalue": 1.79e-49, "gene_start": 1, "gene_end": 81, "reference_start": 1, "reference_end": 81, "reference_sequence": "MTLYDLLELPQTATLQEIKTAYKRLAKRYHPDINKQGADTFVKINNAYAVLSDTTQKAEYDAMLRFSEFEDRVKRLDFSIKWHEQFMEELQFHHNWDFDFIRNREYTQPTPTNNKYSSFLDKDVSLPFYQLYSKASWTLTWKTHFLDVIRSSKPS" }, "paralog": false, "identity": 0.9870967741935484, "score": 309.0, "evalue": 5.56e-110, "description": "Internal stop codon at 2080. Frameshift due to insertion around 2239. Nonsense mutation around 2080.", "psc": { "uniref90_id": "UniRef90_A0AB38W8W2", "product": "Chaperone protein", "uniref50_id": "UniRef50_A0AB38W8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8W2", "UniRef:UniRef50_A0AB38W8W2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8W2" ], "product": "Chaperone protein" } }, "id": "CNOCLNGIAL_44", "locus": "GCA000143945_00002" }, { "type": "cds", "sequence": "contig_1", "start": 2095, "stop": 2358, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGAATTACAGTTCCACCACAACTGGGACTTTGACTTTATCCGCAACAGGGAATACACCCAGCCAACCCCTACCAACAATAAGTATTCTTCCTTTTTAGACAAGGATGTATCCTTGGCCTTTTACCAACTTTACTCCAAGGGCAAGTTGGACTTTGACTTGGAAGACACACTTCTTAGACGTCATTCGATCAAGCAAGCCTTCTTAAAGGGCAAGAAGCTTAACGATGTTTTAAAAGAGCAGTACAACTATTTAGGTTGA", "aa": "MEELQFHHNWDFDFIRNREYTQPTPTNNKYSSFLDKDVSLAFYQLYSKGKLDFDLEDTLLRRHSIKQAFLKGKKLNDVLKEQYNYLG", "aa_hexdigest": "1884f69e138bdc17e4d04b1d4c8b15b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10555.729799999992, "isoelectric_point": 6.824566841125488 }, "id": "CNOCLNGIAL_45", "locus": "GCA000143945_00003" }, { "type": "cds", "sequence": "contig_1", "start": 2416, "stop": 2529, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTGAAGGTGGGGTGGTCAACCTCCCTTTAAAGATCAAAGTCATTTCCAATAACTACCCTGGACAAATGTGGTACGAACTCAACAAGAACTATTCCTTCCGGCTTCTTTGA", "aa": "MREGGVVNLPLKIKVISNNYPGQMWYELNKNYSFRLL", "aa_hexdigest": "a612c54cf5a52c79fd9448aaf9c7bdd7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4386.1042, "isoelectric_point": 9.696383476257324 }, "id": "CNOCLNGIAL_46", "locus": "GCA000143945_00004" }, { "type": "cds", "sequence": "contig_1", "start": 2853, "stop": 4058, "strand": "+", "frame": 3, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P22447", "UniRef:UniRef90_P22447" ], "nt": "ATGGAAGACAATAACAAAACGCAAGCTTACGATTCCAGTAGCATTAAGATTCTTGAAGGCTTAGAGGCCGTGCGTAAGCGCCCCGGCATGTACATCGGTTCCACCGGCGAAGAAGGGTTGCACCACATGATCTGGGAAATCATTGATAACTCAATTGACGAGGCCATGGGTGGCTTTGCGAGTACTGTCAAACTAACCCTTAAAGACAACTTTGTCACCATTGTGGAGGATGATGGCCGGGGCATTCCCGTTGACATCCATCCTAAGACCAACCGCTCCACCGTTGAAACAGTGTTTACGGTACTCCACGCTGGGGGTAAGTTTGACAACGATAGCTATAAGGTGTCAGGGGGACTCCACGGTGTGGGTGCCTCGGTTGTTAATGCTTTAAGTTCCTCCTTTAAGGTATGGGTAGCCAGGGAGCACCAACAGTACTTCCTAGCGTTCCACAACGGTGGGGAAGTGATTGGTGATCTTGTCAACGAAGGTAAGTGTGACAAGGAACACGGTACCAAGGTTGAGTTTGTCCCGGACTTTACCGTGATGGAAAAGAGTGATTACAAACAAACGGTAATTGCTAGTAGGTTGCAACAATTAGCCTTTCTCAACAAAGGGATCCAAATTGACTTTGTTGATGAACGCCGTCAAAATCCGCAAAGCTTTTCTTGGAAGTATGATGGTGGCTTAGTCCAATACATCCACCACCTCAACAACGAAAAGGAACCTTTATTTGAGGACATTATCTTTGGTGAAAAAACCGATACTGTTAAATCAGTTAGCCGTGATGAGAGCTACACAATTAAGGTGGAAGTGGCCTTTCAGTACAACAAGACGTATAACCAATCAATCTTTAGTTTTTGTAACAACATTAATACCACTGAAGGCGGTACCCACGTTGAAGGCTTTCGCAATGCCTTAGTGAAGATCATTAACCGCTTTGCTGTCGAAAACAAGTTCTTAAAGGAAACGGATGAAAAGATTACCCGCGATGACATCTGTGAAGGGTTAACGGCCATTATCTCAATTAAGCACCCCAACCCCCAGTATGAGGGTCAAACCAAAAAGAAATTGGGTAACACCGAAGTGCGTCCTCTAGTTAACAGTATTGTTAGTGAAATCTTTGAGCGCTTTATGTTGGAAAACCCCCCAGGAAGCCAACGCCATTATTCGCAAAACGCTGTTAGCGCAAGAAGCACGGCGCCGTAG", "aa": "MEDNNKTQAYDSSSIKILEGLEAVRKRPGMYIGSTGEEGLHHMIWEIIDNSIDEAMGGFASTVKLTLKDNFVTIVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVAREHQQYFLAFHNGGEVIGDLVNEGKCDKEHGTKVEFVPDFTVMEKSDYKQTVIASRLQQLAFLNKGIQIDFVDERRQNPQSFSWKYDGGLVQYIHHLNNEKEPLFEDIIFGEKTDTVKSVSRDESYTIKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKETDEKITRDDICEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSIVSEIFERFMLENPPGSQRHYSQNAVSARSTAP", "aa_hexdigest": "bb81f462640458c3f98d9f941be3e93b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00113D9B84", "query_cov": 0.9875311720698254, "subject_cov": 0.9565217391304348, "identity": 0.518, "score": 385.0, "evalue": 8.12e-129, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A9D9C2D1" }, "genes": [ "gyrB" ], "truncated": "3-prime", "pseudogene": { "start": 2853, "stop": 4359, "observations": { "insertion": [ 3996 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P22447", "query_cov": 0.9526184538653366, "subject_cov": 0.5876923076923077, "identity": 0.885, "score": 694.0, "evalue": 5.6e-247, "gene_start": 1, "gene_end": 382, "reference_start": 1, "reference_end": 382, "reference_sequence": "MEENNKANIYDSSSIKVLEGLEAVRKRPGMYIGSTGEEGLHHMIWEIVDNSIDEAMGGFASFVKLTLEDNFVTRVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVFRQNKKYFLSFSDGGKVIGDLVQEGNSEKEHGTIVEFVPDFSVMEKSDYKQTVIVSRLQQLAFLNKGIRIDFVDNRKQNPQSFSWKYDGGLVEYIHHLNNEKEPLFNEVIADEKTETVKAVNRDENYTVKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKDSDEKINRDDVCEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSVVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSNFEQIFNNAEISALVMAIGCGIKPDFELEKLRYSKIVIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIFIAQPPLYKVSYSHKDLYMHTDVQLEQWKSQNPNVKFGLQRYKGLGEMDALQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEKNARSVKNIDI" }, "paralog": false, "identity": 0.906187624750499, "score": 919.0, "evalue": 0.0, "description": "Frameshift due to insertion around 3996.", "psc": { "uniref90_id": "UniRef90_P22447", "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P22447", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P22447", "EC:5.6.2.2", "UniRef:UniRef50_P22447", "COG:COG0187", "COG:L", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P22447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P22447" ], "product": "DNA gyrase subunit B" } }, "id": "CNOCLNGIAL_47", "locus": "GCA000143945_00005" }, { "type": "cds", "sequence": "contig_1", "start": 4261, "stop": 4806, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7Y1SYY1" ], "nt": "GTGGAAAAGTCCCACTTTGAACAAATCTTTAATAATGTGGAAATCTCGGCTTTAGTCATGGCCGTTGGCTGTGGCATTAAACCAGACTTCGAGTTGGAAAAACTGCGGTATAACAAGATCATTATCATGACCGATGCTGATGTCGATGGGGCGCACATCCGTACCCTCCTTTTAACCTTCTTCTTTCGCTTTATGTATCCCTTGGTGGAACAGGGCAACATTTACATTGCCCAACCCCCACTGTATAAGGTCTCTTACTCTAATAAGGACTTATACATGCAAACCGATGTCCAACTAGAGGAGTGGAAGCAACAACACCCTAATCTGAAATACAACTTACAACGCTACAAGGGTTTAGGGGAAATGGATGCCATCCAACTGTGGGAAACCACGATGGATCCCAAGGTGCGTACCTTACTAAAGGTAACCGTTGAAGATGCTTCAATTGCTGATAAAGCCTTCTCCTTGTTAATGGGCGATGAAGTTCCCCCAAGACGGGAATTCATCGAACAAAACGCACGTAACGTAAAGAACATTGATATCTAA", "aa": "MEKSHFEQIFNNVEISALVMAVGCGIKPDFELEKLRYNKIIIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIYIAQPPLYKVSYSNKDLYMQTDVQLEEWKQQHPNLKYNLQRYKGLGEMDAIQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEQNARNVKNIDI", "aa_hexdigest": "782f69594eecd4393f3b2cfcd0e817ab", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A316RL35", "query_cov": 1.011049723756906, "subject_cov": 0.8672985781990521, "identity": 0.596, "score": 216.0, "evalue": 2.09e-68, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A7Y1SYY1" }, "pscc": { "uniref50_id": "UniRef50_A0A7Y1SYY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7Y1SYY1" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "CNOCLNGIAL_48", "locus": "GCA000143945_00006" }, { "type": "cds", "sequence": "contig_1", "start": 4806, "stop": 7325, "strand": "+", "frame": 3, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335", "KEGG:K02469", "RefSeq:WP_014574838.1", "SO:0001217", "UniParc:UPI0001DD643F", "UniRef:UniRef100_A0AAX0SQM1", "UniRef:UniRef50_P47719", "UniRef:UniRef90_P22446" ], "nt": "ATGGCAAAGCAACAAGATCAAATTGACAAAATTCGTCAGGAATTAGCACAATCAGCAATTAAAAACATCTCCCTCTCCAGTGAACTAGAACGTTCCTTCATGGAGTATGCGATGTCGGTGATAGTGGCTCGCGCGCTCCCAGATGCGAGGGATGGACTTAAACCTGTGCACCGCCGTGTGCTCTATGGTGCTTACACTGGGGGCATGCACCACGATCGCCCCTTTAAAAAGTCAGCACGGATTGTCGGTGATGTAATGAGTAAGTTCCACCCCCACGGGGACATGGCCATTTACGACACAATGTCGCGGATGGCGCAGGACTTTTCTTTGCGTTACCTCTTAATTGACGGACATGGTAACTTTGGCTCGATTGATGGCGATCGTCCCGCAGCACAACGTTATACGGAAGCACGGTTGTCGAAATTAGCGGGGGAACTGTTACGTGACATTGACAAGGACACGGTCGACTTTGTCGCTAACTATGATGGGGAAGAACAAGAACCAACGGTTTTACCAGCTGCCTTTCCTAATTTATTAGCTAATGGTTCGAGTGGGATTGCGGTAGGGATGTCCACTTCCATTCCGTCTCACAACTTATCGGAGTTAATCCAGGGCTTAATCCTTTTAATTGACAACCCTGACTGCACCATTAATGATTTATTAGGTGTCATTAAAGGACCGGACTTTCCCACCGGAGCCAACATTATTTACACCAAGGGGATTGAAAGCTACTTTGAAACTGGGAAGGGTAATGTGGTCATTCGTTCTAAAGTCAGCATTGAACAACTACCAACCCGGGCTGCTTTAGTAGTCACGGAAATTCCCTACATGGTCAACAAGACCAGTTTAATTGAAAAGATTGTCGAGTTAGTCAAGGCTGAGGAAATCACCGGGATTGCCGATATCCGTGATGAGTCATCCAGGGAAGGAATTCGCTTGGTGATTGAAGTGAAGCGCGACACCGTTCCGGAAGTCTTATTAAACCAACTGTTCAAATCAACCCGTTTACAGGTGCGGTTCCCCGTGAACATGTTGGCACTGGTTAAGGGTGCACCTAAATTGCTCAACATGAAGCAAGCGTTAACGGTTTACCTCGAGCACCAACTCGATGTTCTGATTCGCAAAACCCAGTTTAACCTCAAGAAGTACCAGGAGCGCTTTCATATCTTAAGTGGCCTTTTAATTGCGGCGTTGAACATTGATGAGGTAATTGCGATCATTAAAAAGTCCGCTAATAACCAGGTAGCAATGGAAGCACTCCACGAACGCTTCGGGTTAGATGAAATCCAAGCACGGGCGGTTCTGGACATGCGTTTACGTAGCTTAAGTGTCTTGGAAGTCAATAAACTACAAACCGAACAACAAGAGTTGAAAGCTTTAATTGAGTTCTGTCAACAGGTGTTAGCTGACAAACAACTGCAACTCAAACTAATTAAGGAGCAGTTAACCAAGATTAACGAACAGTTTGGCGATCCACGCCGTAGTGAAATCCTGTACGGTATTAGTGAAGACATTGATGATGAGGACTTAATTACCCAGGAAAACGTGGTAATCACGATGTCCACTAACGGTTATCTCAAACGGATTGGGGTAGACTCTTATAACCTGCAACACCGTGGTGGGGTGGGTGTTAAGGGTTTAACTACCTACACCGATGACAGTATTAGCCAGCTGTTAGTCTGCTCGACCCACTCGGACCTCTTATTCTTTACGGACAAGGGAAAGGTCTACCGCATCCGGGCCCACCAAATTCCCCCGGGTTTTCGTACTAACAAGGGGATTCCGGCGGTTAACCTGATTAAGATCGACAAGGACGAGAAGATCTGTGCCTTAATTTCGGTGAATGATTACCAAAATGGTTACTTCTTCTTTTGTACCAAGAACGGTACCATTAAGCGCACCAGTCTAAGTGAGTTTGCCAACATCTTGAGTATTGGTAAACGCGCCATCCTCTTTAAGGAAAACGATGTTTTGTTCTCGGTTATTCGCACCAGTGGTCAAGATGACATCTTTATCGGTTCGACCGCGGGGTTTGTCGTGCGTTTCCACGAAGACACCGTCCGTCCGCTGTCCCGCGCTGCCATGGGGGTCTTGGGGATTAATCTCAACCAGGGCGAATTTGTCAATGGTCTGTCCACTTCCAGTAACGGGAGTTTATTACTCTCTGTGGGTCAAAATGGCATTGGTAAGCTCACCAGTATTGACAAATATCGTTTAACCAAGCGGAATGCTAAGGGAGTTAAAACACTACGAGTAACTGCTAAAACTGGTCCGGTTGTGACCACTACGACCGTCTTTGGTAACGAAGATTTGTTAATGATTTCTTCAGCTGGTAAGATTGTCCGGATTAGTCTGGAACAACTTTCAGAACAGGGAAAAAACACTTCTGGTGTTAAACTAATCAAACTAAAAGAGAAGGAACGCTTAGAGACTGTTACTATCTTTAAAAAGGAAGAAGCCATTAAAACCACAACAGCAACCGAGACAGACGATGTTGGATCGAAACAAATTACGCAATAA", "aa": "MAKQQDQIDKIRQELAQSAIKNISLSSELERSFMEYAMSVIVARALPDARDGLKPVHRRVLYGAYTGGMHHDRPFKKSARIVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGNFGSIDGDRPAAQRYTEARLSKLAGELLRDIDKDTVDFVANYDGEEQEPTVLPAAFPNLLANGSSGIAVGMSTSIPSHNLSELIQGLILLIDNPDCTINDLLGVIKGPDFPTGANIIYTKGIESYFETGKGNVVIRSKVSIEQLPTRAALVVTEIPYMVNKTSLIEKIVELVKAEEITGIADIRDESSREGIRLVIEVKRDTVPEVLLNQLFKSTRLQVRFPVNMLALVKGAPKLLNMKQALTVYLEHQLDVLIRKTQFNLKKYQERFHILSGLLIAALNIDEVIAIIKKSANNQVAMEALHERFGLDEIQARAVLDMRLRSLSVLEVNKLQTEQQELKALIEFCQQVLADKQLQLKLIKEQLTKINEQFGDPRRSEILYGISEDIDDEDLITQENVVITMSTNGYLKRIGVDSYNLQHRGGVGVKGLTTYTDDSISQLLVCSTHSDLLFFTDKGKVYRIRAHQIPPGFRTNKGIPAVNLIKIDKDEKICALISVNDYQNGYFFFCTKNGTIKRTSLSEFANILSIGKRAILFKENDVLFSVIRTSGQDDIFIGSTAGFVVRFHEDTVRPLSRAAMGVLGINLNQGEFVNGLSTSSNGSLLLSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTAKTGPVVTTTTVFGNEDLLMISSAGKIVRISLEQLSEQGKNTSGVKLIKLKEKERLETVTIFKKEEAIKTTTATETDDVGSKQITQ", "aa_hexdigest": "6519b9e49c1722c59e92d5f6fcd7da89", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD643F", "ncbi_nrp_id": "WP_014574838.1", "uniref100_id": "UniRef100_A0AAX0SQM1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD643F", "RefSeq:WP_014574838.1", "UniRef:UniRef100_A0AAX0SQM1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQM1", "uniref90_id": "UniRef90_P22446" }, "psc": { "uniref90_id": "UniRef90_P22446", "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47719", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47719", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47719" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "CNOCLNGIAL_49", "locus": "GCA000143945_00007" }, { "type": "cds", "sequence": "contig_1", "start": 7297, "stop": 7860, "strand": "+", "frame": 1, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_P47251", "UniRef:UniRef90_P75107" ], "nt": "ATGTTGGATCGAAACAAATTACGCAATAACCTTGACTTCTTTAAAAAGAAGTTAGTTGAACGGGGTGTAAGTGAAAGCCAGTTCGAGGCGTACGTGCAAGCTGATAAAGCAATGCGCAAACTGTTGCACCAAATTGAACTGGCCAACCAAAAGCAAACCCTGTTAGCGAAACAGGTTGCCAAGAAGAAGGGTGATCTGAAGTTATTAAAGGAATCCAAGGAGTTAAAGCAAAAACTAGAACAGCTCAACATCGCTTTTAAGGAAGCGGAAACCTTAAGCCAAGAGTTACTGTTAAACCTCCCTAACATTGCTGATGAATCAGTACCAGTTGGACGGGATGAAACTGCTAACTTAGAACTGCTCAAAGAAGGGCGTAAACCGGTGTTTGACTTTACTCCCTTACCCCACTGGGAGTTGTGTGAACGCTTACAGTTAGTTGCCTTTGATAAGGCCACTAAGCTAACGGGTGCACGGTTTGTCGCTTACACTGATAAAGCAGCGAAACTGCTCCGCGCCATTGCTAGCTTAATGATTGACTTCAACAAAAACAAATACCAGGAGTGA", "aa": "MLDRNKLRNNLDFFKKKLVERGVSESQFEAYVQADKAMRKLLHQIELANQKQTLLAKQVAKKKGDLKLLKESKELKQKLEQLNIAFKEAETLSQELLLNLPNIADESVPVGRDETANLELLKEGRKPVFDFTPLPHWELCERLQLVAFDKATKLTGARFVAYTDKAAKLLRAIASLMIDFNKNKYQE", "aa_hexdigest": "fa3c4a06c3b28842259c6adc9c566d7e", "start_type": "ATG", "rbs_motif": null, "genes": [ "serS" ], "truncated": "3-prime", "pseudogene": { "start": 7297, "stop": 8163, "observations": { "mutation": [ 7858 ], "stop": [ 7858 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75107", "query_cov": 1.0, "subject_cov": 0.4452380952380952, "identity": 0.9840000000000001, "score": 343.0, "evalue": 1.85e-115, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MLDRNKLRNNLDFFKKKLVERGVSESQFEAYVQADKAMRKLLHQIELANQKQTLLAQQVAKKKGDPKLLKESKELKQKLEQLNIAFKEAETLSQELLLNLPNIADESVPVGRDETANLELLKEGRKPVFDFTPLPHWELCERLQLVAFDKATKLTGARFVAYTDKAAKLLRAIASLMIDLNKNKYQEWNVPVIVNETSLTGTGQLPKFKDDVFKLENTRYYLSPTLEVQLANLHANEIFTEGELPKYYTATGVNFRQEAGSAGKQTKGTIRLHQFQKVELVKFCKPSEAIHELEEMTRDAEQILLELKIPFRRLLLCSGDMGFSAQKTYDLEVWMAGCNEYREVSSCSSCGDFQARRAMIRYKDLTTGKNTYVATLNGTALAIDRIFAAILEHYQTKAGEVMIPQALLKYLDFDKITKPK" }, "paralog": false, "identity": 0.9861111111111112, "score": 555.0, "evalue": 2.51e-201, "description": "Internal stop codon at 7858. Nonsense mutation around 7858.", "psc": { "uniref90_id": "UniRef90_P75107", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_P47251", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75107", "EC:6.1.1.11", "UniRef:UniRef50_P47251", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ] }, "pscc": { "uniref50_id": "UniRef50_P47251", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47251" ], "product": "Serine--tRNA ligase" } }, "id": "CNOCLNGIAL_50", "locus": "GCA000143945_00008" }, { "type": "cds", "sequence": "contig_1", "start": 7864, "stop": 8559, "strand": "+", "frame": 1, "gene": null, "product": "Serine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A540V7F7" ], "nt": "GTGCCGGTCATTGTGAACGAGACTAGTCTTACTGGTACGGGTCAGCTACCCAAATTTAAGGATGATGTCTTTAAGTTAGAAAACACGCGTTACTACCTCTCCCCGACCCTAGAGGTGCAACTAGCCAACCTCCATGCCAACGAAATCTTCACGGAGGGTGAACTCCCCAAATACTATACCGCTACTGGGGTGAACTTTCGCCAGGAAGCGGGCAGTGCTGGTAAACAAACTAAGGGTACGATTCGCCTACACCAGTTCCAAAAGGTAGAACTAGTGAAGTTTTGTAAACCAAGTGAAGCGATTCACGAACTGGAAGAAATGACCCGGGACGCTGAGCAAATTTTGTTAGAACTCAAAATTCCCTTTCGCCGTTTGTTACTCTGTAGCGGTGACATGGGTTTTAGTGCCCAGAAAACCTATGACTTGGAAGTGTGGATGGCGGGTTGCAATGAGTACCGTGAAGTTTCGTCCTGTTCTTCTTGTGGTGACTTTCAAGCACGCAGAGCAATGATCCGCTATAAGGACTTAACCACAGGCAAGAATACCTATGTGGCTACCTTAAACGGCACCGCTTTAGCGATTGACCGCATCTTTGCAGCCATCTTGGAACACTACCAAACGAAGGCAGGGGAAGTAATGATTCCCCAAGCCTTGTTGAAATACTTAGACTTTGACAAAATTACCAAACCAAAATAA", "aa": "MPVIVNETSLTGTGQLPKFKDDVFKLENTRYYLSPTLEVQLANLHANEIFTEGELPKYYTATGVNFRQEAGSAGKQTKGTIRLHQFQKVELVKFCKPSEAIHELEEMTRDAEQILLELKIPFRRLLLCSGDMGFSAQKTYDLEVWMAGCNEYREVSSCSSCGDFQARRAMIRYKDLTTGKNTYVATLNGTALAIDRIFAAILEHYQTKAGEVMIPQALLKYLDFDKITKPK", "aa_hexdigest": "8ec45be77fe2508c618d0add3afe44e1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4FJT5", "query_cov": 0.9567099567099567, "subject_cov": 0.8804780876494024, "identity": 0.588, "score": 255.0, "evalue": 1.68e-82, "valid": false, "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_A0A540V7F7", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_A0A540V7F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A540V7F7" ], "product": "Serine--tRNA ligase" }, "genes": [], "id": "CNOCLNGIAL_51", "locus": "GCA000143945_00009" }, { "type": "cds", "sequence": "contig_1", "start": 8564, "stop": 8860, "strand": "+", "frame": 2, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_P47252", "UniRef:UniRef90_P75106" ], "nt": "ATGAAACAAGGCGTGTTTGTGGCCATAGAGGGCGTGGATGGTGCGGGGAAAACCGTCCTCCTAGAAGCTTTTAAACAGCGCTTTCCCCAAAGCTTTTTGGGCTTTAAAACGCTTTTTAGCAGGGAACCCGGGGGCACACCGTTAGCGGAAAAAATCCGGGCACTGCTCCTGCATGAAGCAATGGAACCACTTACAGAAGCTTATCTGTTTGCGGCATCGCGCACCGAACACGTGCGCCAGTTAATCCAACCAGCGTTACAACAAAAACAGTTAGTGATAGTAGACCGCTTTGTGTGA", "aa": "MKQGVFVAIEGVDGAGKTVLLEAFKQRFPQSFLGFKTLFSREPGGTPLAEKIRALLLHEAMEPLTEAYLFAASRTEHVRQLIQPALQQKQLVIVDRFV", "aa_hexdigest": "2021ebc7f345e281ef75aac8228ee1cf", "start_type": "ATG", "rbs_motif": null, "genes": [ "tmk" ], "truncated": "3-prime", "pseudogene": { "start": 8564, "stop": 9163, "observations": { "mutation": [ 8858 ], "stop": [ 8858 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75106", "query_cov": 1.0, "subject_cov": 0.4666666666666667, "identity": 1.0, "score": 191.0, "evalue": 3.93e-60, "gene_start": 1, "gene_end": 98, "reference_start": 1, "reference_end": 98, "reference_sequence": "MKQGVFVAIEGVDGAGKTVLLEAFKQRFPQSFLGFKTLFSREPGGTPLAEKIRALLLHEAMEPLTEAYLFAASRTEHVRQLIQPALQQKQLVIVDRFVWSSYAYQGLIKKVGLDVVKKLNADAVGDSMPDFTFIVDCDFETALNRMAKRGQDNLLDNTVKKQADFNTMRQYYHSLVDNKRVFLLDGQNQTGCLEQFIEQLSQCLTQPTLS" }, "paralog": false, "identity": 0.9949748743718593, "score": 390.0, "evalue": 3.98e-141, "description": "Internal stop codon at 8858. Nonsense mutation around 8858.", "psc": { "uniref90_id": "UniRef90_P75106", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_P47252", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75106", "EC:2.7.4.9", "UniRef:UniRef50_P47252", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_P47252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47252" ], "product": "Thymidylate kinase" } }, "id": "CNOCLNGIAL_52", "locus": "GCA000143945_00010" }, { "type": "cds", "sequence": "contig_1", "start": 8906, "stop": 9196, "strand": "+", "frame": 2, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_P47252", "UniRef:UniRef90_P75106" ], "nt": "GTGGTCAAAAAGCTCAATGCTGATGCGGTGGGGGATAGTATGCCCGACTTTACCTTTATTGTTGATTGTGACTTTGAGACTGCTTTAAACCGGATGGCCAAACGCGGTCAGGACAATTTATTGGACAATACTGTCAAAAAGCAAGCGGACTTTAATACTATGCGCCAGTATTACCACAGTTTGGTTGATAACAAACGCGTCTTTCTCTTAGACGGGCAAAACCAAACGGGTTGTCTGGAACAATTTATTGAACAATTAAGCCAATGTTTAACCCAACCCACGCTCTCTTAA", "aa": "MVKKLNADAVGDSMPDFTFIVDCDFETALNRMAKRGQDNLLDNTVKKQADFNTMRQYYHSLVDNKRVFLLDGQNQTGCLEQFIEQLSQCLTQPTLS", "aa_hexdigest": "267853f6b6a38c42afecbf2e79d4afa8", "start_type": "GTG", "rbs_motif": "AAAAA", "genes": [ "tmk" ], "truncated": "5-prime", "pseudogene": { "start": 8606, "stop": 9196, "observations": { "mutation": [ 8858 ], "stop": [ 8858 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75106", "query_cov": 1.0, "subject_cov": 0.45714285714285713, "identity": 0.99, "score": 193.0, "evalue": 4.43e-61, "gene_start": 1, "gene_end": 96, "reference_start": 115, "reference_end": 210, "reference_sequence": "MKQGVFVAIEGVDGAGKTVLLEAFKQRFPQSFLGFKTLFSREPGGTPLAEKIRALLLHEAMEPLTEAYLFAASRTEHVRQLIQPALQQKQLVIVDRFVWSSYAYQGLIKKVGLDVVKKLNADAVGDSMPDFTFIVDCDFETALNRMAKRGQDNLLDNTVKKQADFNTMRQYYHSLVDNKRVFLLDGQNQTGCLEQFIEQLSQCLTQPTLS" }, "paralog": false, "identity": 0.9948979591836735, "score": 385.0, "evalue": 4.99e-139, "description": "Internal stop codon at 8858. Nonsense mutation around 8858.", "psc": { "uniref90_id": "UniRef90_P75106", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_P47252", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75106", "EC:2.7.4.9", "UniRef:UniRef50_P47252", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_P47252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47252" ], "product": "Thymidylate kinase" } }, "id": "CNOCLNGIAL_53", "locus": "GCA000143945_00011" }, { "type": "cds", "sequence": "contig_1", "start": 9169, "stop": 9927, "strand": "+", "frame": 1, "gene": "holB", "product": "DNA polymerase III subunit delta'", "db_xrefs": [ "COG:COG0470", "COG:L", "EC:2.7.7.7", "SO:0001217", "UniRef:UniRef50_P47253", "UniRef:UniRef90_P75105" ], "nt": "ATGTTTAACCCAACCCACGCTCTCTTAATTATTCAAAGACGGGGTAGCTATCTCCAACCAGTTTTAACGGAGTACCTAACCCGGGTCGTTTGTGAACAGCAAACGGGGTGTCAAACATGTCCGAGTTGCTTGGAAATTCTCCATGGTACTTACAACAACTTTTACAGTTTTGACCAAGCCAACCCCTTTAAAAGGGAACATGCCTTGCACCTTAGTGAAGTGTTGAACCGCCAAAGTGAATCCAACCAAAAACAGCTGTACCTGATTAAAAACTTAGAAACGCTCACCGCTACGGCCATGAACAGCTTGCTCCGTTTAATTGAGGAACACCCGGTGAATACCTATGGTGTTTTTACCACGAAAAACGAAAACATGATCTTACCCACCATTTTGAGTCGCGTCCAAAAAGTAGTACTCAAAAAAGCGACCCAGTTACCGTTCCAAGTGGACAGTAAGGACCAGGCGATTCTCAAAAGTTTCTTTAGTGTAGATGAACAGTTACAAGCATTGGACAACGGTAGTTTTACCCGCTTAAAAACGATTATTACGACACTTACCAATAAGAAAAACACAGCGAGCACGGTTCACAAGGCTTGGGTGTTGTTTAAACAATTAAACCAAACGGAAACGGCTCAAGTACTGAACTTTATGGTGGATTACACCAAGGACTTAACCAAAAAGGACCGCTTGTTAAACATGGTGCAAAATTTGGTGTTTAACCCACCGAAAGCAGCGTTGTTTGCTAACCTCATTAACTGA", "aa": "MFNPTHALLIIQRRGSYLQPVLTEYLTRVVCEQQTGCQTCPSCLEILHGTYNNFYSFDQANPFKREHALHLSEVLNRQSESNQKQLYLIKNLETLTATAMNSLLRLIEEHPVNTYGVFTTKNENMILPTILSRVQKVVLKKATQLPFQVDSKDQAILKSFFSVDEQLQALDNGSFTRLKTIITTLTNKKNTASTVHKAWVLFKQLNQTETAQVLNFMVDYTKDLTKKDRLLNMVQNLVFNPPKAALFANLIN", "aa_hexdigest": "5057d37fe88f8afd130232460ea4c1c1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75105", "query_cov": 1.0, "subject_cov": 0.9960474308300395, "identity": 0.996, "score": 493.0, "evalue": 5.08e-176, "valid": true, "gene": "HolB", "product": "DNA polymerase III subunit delta'", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47253", "cog_id": "COG0470", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_P47253", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47253" ], "product": "Uncharacterized protein MG007" }, "genes": [ "holB" ], "id": "CNOCLNGIAL_54", "locus": "GCA000143945_00012" }, { "type": "cds", "sequence": "contig_1", "start": 9932, "stop": 11260, "strand": "+", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "EC:3.6.-.-", "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872", "RefSeq:WP_014325281.1", "SO:0001217", "UniParc:UPI0001DD6442", "UniRef:UniRef100_A0AAV5N6V1", "UniRef:UniRef50_P47254", "UniRef:UniRef90_P75104" ], "nt": "ATGGACACCAAACAAACCATGTTTGCCCTCGCTACTGCGCCCTTCAACAGTGCCATTCACATTATTAGGTTATCTGGTCCCGATGTTTATCGCATTATTAACCAAATAACCAATAAGGAAGTCAAACCGTTGGGTATGCGGATTCAACGCGTTTGGTTAATTGATCACAACCAGAAAAAAGTGGATGATGTGTTGCTGTTTAAATTTGTCGCACCTAACTCTTACACTGGGGAAGATCTGATTGAAATTTCGTGCCATGGCAGCATGGTAATAGTCAACGAAATTATTGGTTTATTGTTAAAGCACGGGGCGGTACAAGCCCAACCCGGGGAGTTTACGCAGCGCGGCTATCTCAACGGTAAGATGAGTTTAAACCAAGCGGCTTCTGTTAATAACTTAGTACTTTCACCGAATACAACCCTAAAGGATGTCGCTTTAAATGCGTTAGCTGGTCAAGTTGATGCCAGGTTAGAACCCTTAGTGGAAAAGTTGGGTCAACTAGTAATGCAAATGGAAGTGAACTTGGATTACCCCGAGTACACTGATGAACAGCGCGAACTGGTGACAATGAACCAAGCAGTCTTGCAAATAACCCAAATCTTAAACCAAATCGTGGTTGGTCAAGACCAGTTACAACGGCTCAAAGATCCGTTTAAGATTGCCATTATTGGTAATACTAATGTTGGTAAGTCCTCCTTATTGAATGCTCTGTTAGATCAAGATAAAGCGATAGTATCCGCCATTAAAGGCAGTACCCGTGACATTGTTGAGGGTGACTTTGCTCTAAACGGTCACTTCGTCAAAATCCTCGACACAGCGGGAATTCGCCAACACCAAAGTGCGCTCGAAAAAGCGGGCATTCAAAAAACCTTTGGCGCCATTAAAACCGCTAACTTAGTAATTTACCTTTTGGATGCCCGGCAGCCAGAACCTGATCCCAAGATTATTGCGCGATTAAAGAAGCTGAAGAAGGACTTCTTTTTGGTCCACAATAAAGCAGACTTAGTGCAGCAAAGCTTTCAGGTAAGTATTTCTGCCAAACAAAAACAGATCCAACCACTTGTCGATTTATTAACCCAATACCTACACCAGTTTTACAGTGTGGAGCAGAACCAGCTCTACTTAATTAGTGATTGGCAAACGATCCTGCTCCAAAAAGCAATAGCCGAACTAGAACATTTTTTAATTAAACAACAAAACTGCCTTTTCTTTGATATCCTAGTTGTTCATTTACGTGCTGCTCACGAATACATTCTCCAAGTCTTGGGTAAAAACACCAACTATGACCTGATTAACGAAATCTTTAAGCACTTTTGCTTAGGTAAGTAA", "aa": "MDTKQTMFALATAPFNSAIHIIRLSGPDVYRIINQITNKEVKPLGMRIQRVWLIDHNQKKVDDVLLFKFVAPNSYTGEDLIEISCHGSMVIVNEIIGLLLKHGAVQAQPGEFTQRGYLNGKMSLNQAASVNNLVLSPNTTLKDVALNALAGQVDARLEPLVEKLGQLVMQMEVNLDYPEYTDEQRELVTMNQAVLQITQILNQIVVGQDQLQRLKDPFKIAIIGNTNVGKSSLLNALLDQDKAIVSAIKGSTRDIVEGDFALNGHFVKILDTAGIRQHQSALEKAGIQKTFGAIKTANLVIYLLDARQPEPDPKIIARLKKLKKDFFLVHNKADLVQQSFQVSISAKQKQIQPLVDLLTQYLHQFYSVEQNQLYLISDWQTILLQKAIAELEHFLIKQQNCLFFDILVVHLRAAHEYILQVLGKNTNYDLINEIFKHFCLGK", "aa_hexdigest": "a72c9d6a2c9cf09ed7ea4eb120148f99", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6442", "ncbi_nrp_id": "WP_014325281.1", "uniref100_id": "UniRef100_A0AAV5N6V1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6442", "RefSeq:WP_014325281.1", "UniRef:UniRef100_A0AAV5N6V1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N6V1", "uniref90_id": "UniRef90_P75104" }, "psc": { "uniref90_id": "UniRef90_P75104", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "ec_ids": [ "3.6.-.-" ], "uniref50_id": "UniRef50_P47254", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47254" ], "product": "tRNA modification GTPase MnmE" }, "genes": [ "mnmE" ], "id": "CNOCLNGIAL_55", "locus": "GCA000143945_00013" }, { "type": "cds", "sequence": "contig_1", "start": 11260, "stop": 11937, "strand": "+", "frame": 1, "gene": "tatD", "product": "putative metal-dependent hydrolase MPN_009", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.-.-", "GO:0004536", "GO:0005829", "GO:0046872", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_P47255", "UniRef:UniRef90_P78005" ], "nt": "ATGGAATACTTTGATGCGCACTGTCACTTAAACTGTGAACCCCTACTGGAACAGCACGAAAAGAGTTTGGCCAACTTTAGGTTAATTGGGTTAAAGGCCAATGTTGTGGGCACTAATTTAACTAACTCCCAAATTGCGGTGAATTTAGCTAAACAGCACCCTGATTTACTCAAGGCAGGGGTGGGTATTCACCCTAATGATGTCCAGCTTTTTGATTTAAAAGCAGCGCAAGCAACACTAGAAAAATTAGTAAGTACGCATCGCTCATTCATTAGTTGTATTGGTGAATATGGCTTTGATTACCACTACACTAAGGACTACATTACCCAACAAGAACAGTTCTTTTTGATGCAGTTCCAACTAGCAGAACAGTACCAGTTAGTGCACATGTTGCATGTCCGTGATGTGCACGAAAGGATATACGAAGTTTTGAAAAGGTTAAAACCTAAACAACCAGTGGTGTTTCACTGTTTTAGTGAAGACACCAATACTGCTTTAAAGCTGCTAACTTTAAGAGAAGTAGGACTAAAGGTTTATTTTTCGATTCCGGGGATTGTGACCTTTAAGAATGCGAAAAACTTGCAAGCCGCTTTATCAGTTATTCCAACAGAGCTACTTTTAAGTGAAACCGATTCGCCTTATTTAGCACCAGTACCTTTTCGGGGCAAAACTAATTGA", "aa": "MEYFDAHCHLNCEPLLEQHEKSLANFRLIGLKANVVGTNLTNSQIAVNLAKQHPDLLKAGVGIHPNDVQLFDLKAAQATLEKLVSTHRSFISCIGEYGFDYHYTKDYITQQEQFFLMQFQLAEQYQLVHMLHVRDVHERIYEVLKRLKPKQPVVFHCFSEDTNTALKLLTLREVGLKVYFSIPGIVTFKNAKNLQAALSVIPTELLLSETDSPYLAPVPFRGKTN", "aa_hexdigest": "446ae871dd19e49f0a599f40c2e6e3ca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P78005", "query_cov": 1.0044444444444445, "subject_cov": 0.8625954198473282, "identity": 0.991, "score": 443.0, "evalue": 1.09e-156, "valid": true, "gene": "TatD", "product": "Uncharacterized metal-dependent hydrolase MPN_009", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47255", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424", "go_ids": [ "GO:0005829", "GO:0004536", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47255" ], "product": "Uncharacterized metal-dependent hydrolase MG009" }, "genes": [ "tatD" ], "id": "CNOCLNGIAL_56", "locus": "GCA000143945_00014" }, { "type": "cds", "sequence": "contig_1", "start": 12242, "stop": 12352, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTACTCGCCTTCGCTTAACGACATTAAGAGCATTTTGAACAAATATACCTCTAAGGACTATGAGTTAAAATGCGAAAATAGGTATGATGGCAAGCTTGAATTATGA", "aa": "MAYSPSLNDIKSILNKYTSKDYELKCENRYDGKLEL", "aa_hexdigest": "ef6ad5242060c2922cad14c787ae9fd9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4243.7669000000005, "isoelectric_point": 6.057411003112793 }, "id": "CNOCLNGIAL_57", "locus": "GCA000143945_00015" }, { "type": "cds", "sequence": "contig_1", "start": 12377, "stop": 12637, "strand": "+", "frame": 2, "gene": null, "product": "DUF16 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6444", "UniRef:UniRef100_A0A0H3DL80", "UniRef:UniRef50_A0A0H3DL80", "UniRef:UniRef90_A0A0H3DL80" ], "nt": "GTGAAAACACCTGGCACTAGGTACGTCACCCATAAGCAACTTGATGAAAAGCTCAAAAATTTTGTCACTAAAACAGAATTTAAAGAGTTCCAAACTGTAGTTATGGAGAGTTTTGCTGTTCAAAACCAGAATATTGATGCTCAGGGTGAGCAGATCAAAGAACTTCAAGTTGAACAAAAAGCTCAAGGAAAGACCCTGCAATTGATACTTGAAGCTCTCCAGGGTATAAACAAACGTCTAGACAACTTAGAGTCCAAATAA", "aa": "MKTPGTRYVTHKQLDEKLKNFVTKTEFKEFQTVVMESFAVQNQNIDAQGEQIKELQVEQKAQGKTLQLILEALQGINKRLDNLESK", "aa_hexdigest": "75de747e0fb9f4f2ad11881c86013141", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6444", "uniref100_id": "UniRef100_A0A0H3DL80", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6444", "UniRef:UniRef100_A0A0H3DL80" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DL80", "uniref90_id": "UniRef90_A0A0H3DL80" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DL80", "product": "DUF16 domain-containing protein", "uniref50_id": "UniRef50_A0A0H3DL80" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DL80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DL80" ], "product": "DUF16 domain-containing protein" }, "genes": [], "id": "CNOCLNGIAL_58", "locus": "GCA000143945_00016" }, { "type": "cds", "sequence": "contig_1", "start": 12945, "stop": 13109, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTATTCTTACAGAGGATTCAATATCCACAAAGGTGACAAACATTATTTCAACAGTTTCCTTACTCTTAAAGCGAATTCATATTTACCATTTACGTCAAAAAATTTTGATGTCTATTCCAAAAGAATTAGGTTATCTGTTTCGTTTCATTGAATACTAA", "aa": "MKIILTEDSISTKVTNIISTVSLLLKRIHIYHLRQKILMSIPKELGYLFRFIEY", "aa_hexdigest": "258acaabdd536dd76234507b2e94fe36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6421.700299999997, "isoelectric_point": 9.774519157409667 }, "id": "CNOCLNGIAL_59", "locus": "GCA000143945_00017" }, { "type": "cds", "sequence": "contig_1", "start": 13285, "stop": 13518, "strand": "-", "frame": 3, "gene": null, "product": "lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019135978", "UniRef:UniRef90_UPI0019135978" ], "nt": "ATGAAATTTAAATTCTTATTAACCCCACTATTATCTTCAGTTTTATTTCTTAGTGCTTGTAGCGCCACTTTCGAAGCTGATCTCAAAAATCTTATTAAGGAAACAGATGGTAAAGACCTTGATGTTTCTAAATTAATTATTACTTCGGAAGGGAAACAAATCTTAATAGGTTATTTAAAAAAATCTTACGAAGTTAACTCTGAAAAAACTACTGAACTATTACTAAATGCCTGA", "aa": "MKFKFLLTPLLSSVLFLSACSATFEADLKNLIKETDGKDLDVSKLIITSEGKQILIGYLKKSYEVNSEKTTELLLNA", "aa_hexdigest": "e623a3d940a2248e5c58d780707e6d4c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 13225, "stop": 13503, "observations": { "mutation": [ 13239, 13287 ], "stop": [ 13239, 13287 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019135978", "query_cov": 0.935064935064935, "subject_cov": 0.7741935483870968, "identity": 1.0, "score": 135.0, "evalue": 5.17e-40, "gene_start": 6, "gene_end": 77, "reference_start": 2, "reference_end": 73, "reference_sequence": "MLLTPLLSSVLFLSACSATFEADLKNLIKETDGKDLDVSKLIITSEGKQILIGYLKKSYEVNSEKTTELLLNAWKQSQEKNQIGIDLFNWTKS" }, "paralog": false, "identity": 0.9565217391304348, "score": 166.0, "evalue": 1.22e-54, "description": "Internal stop codon at 13239, 13287. Nonsense mutation around 13239, 13287.", "psc": { "uniref90_id": "UniRef90_UPI0019135978", "product": "lipoprotein", "uniref50_id": "UniRef50_UPI0019135978", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019135978", "UniRef:UniRef50_UPI0019135978" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0019135978", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019135978" ], "product": "lipoprotein" } }, "id": "CNOCLNGIAL_60", "locus": "GCA000143945_00018" }, { "type": "cds", "sequence": "contig_1", "start": 13690, "stop": 13902, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATCAAGATCTTAATAATTTTTCGATAAATGCTAAGTTGAACTATAACTTTAATTGGCATGGAAATTATTCTTCCAATGGGTTTGCTGCCAAAAAAGGTGACAAACATGTTTTTGATCTTCCTCTTGCAATTAAGCCATCTTCAAAGAACCAATCTAAAACGATAAGTTTCATTACTAAGACAGGGGAAGGCGTTGACACAGAATGA", "aa": "MEYQDLNNFSINAKLNYNFNWHGNYSSNGFAAKKGDKHVFDLPLAIKPSSKNQSKTISFITKTGEGVDTE", "aa_hexdigest": "a387d55e91d6b487754fcba3a42cbbf1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7872.618200000002, "isoelectric_point": 8.00570011138916 }, "id": "CNOCLNGIAL_61", "locus": "GCA000143945_00019" }, { "type": "cds", "sequence": "contig_1", "start": 14020, "stop": 14250, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCEF156", "PFAM:PF00938.23" ], "nt": "ATGAAATTTAAATACCTTTCAATTCCGTTTTTATCTTCAACTTTATTGTTTAGTGCTTATGCTTCTATCCAGTCTGATCTAAGAAATCTTATTCAAGAAACCACTAAACAAGACGTTGATGTGTACAAAGTAATCAAAACTTCTGAAGGCAGAAAAAACTTAATAACTTCTCTAAAGAAATCTTACGAAGTTAATCCTGATAAAACCACAAAACTATTGTTAGATGCCTGA", "aa": "MKFKYLSIPFLSSTLLFSAYASIQSDLRNLIQETTKQDVDVYKVIKTSEGRKNLITSLKKSYEVNPDKTTKLLLDA", "aa_hexdigest": "7003eb05d30df8ae726557f53f8a5aab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCEF156", "query_cov": 0.9473684210526315, "subject_cov": 0.9863013698630136, "identity": 0.708, "score": 94.7, "evalue": 4.09e-24, "valid": false, "uniref50_id": "UniRef50_UPI001FCEF156" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCEF156", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCEF156" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 32, "aa_cov": 0.42105263157894735, "hmm_cov": 1.0, "evalue": 1.3498092509371095e-06, "score": 23.492643356323242, "start": 45, "stop": 76 } ], "seq_stats": { "molecular_weight": 8689.978299999995, "isoelectric_point": 9.435415458679199 }, "id": "CNOCLNGIAL_62", "locus": "GCA000143945_00020" }, { "type": "cds", "sequence": "contig_1", "start": 14763, "stop": 14978, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F9400A", "UniRef:UniRef100_A0AB38W8J2", "UniRef:UniRef50_A0AB38W8J2", "UniRef:UniRef90_A0AB38W8J2" ], "nt": "ATGTTGTTTTGTTTGAAGTCTCTTTACTTTAAATTGCAAAAATTTTTAGCAATTTTATTGGCAGTTTTTGAAATTCTGGTCAATTTATTTAGTGAAGGAAAAATTATGTCTTTACTCAATATATTTATTAGATCAACGATGATTTTGATCCTTGCTGGCAACAAAGTTGCACGTTTTAGTTTTCTAAACCTTGAGCAGAAAGGAGGAATTTATTAA", "aa": "MLFCLKSLYFKLQKFLAILLAVFEILVNLFSEGKIMSLLNIFIRSTMILILAGNKVARFSFLNLEQKGGIY", "aa_hexdigest": "05c35bec620a92818791b77a240368a2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002F9400A", "uniref100_id": "UniRef100_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F9400A", "UniRef:UniRef100_A0AB38W8J2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8J2", "uniref90_id": "UniRef90_A0AB38W8J2" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8J2", "uniref50_id": "UniRef50_A0AB38W8J2" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8J2" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 14685, "stop": 14978, "observations": { "mutation": [ 14745 ], "stop": [ 14745 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8J2", "query_cov": 1.0, "subject_cov": 0.7319587628865979, "identity": 0.986, "score": 126.0, "evalue": 1.46e-36, "gene_start": 1, "gene_end": 71, "reference_start": 27, "reference_end": 97, "reference_sequence": "HLRDVNTSLMFLNTLKNLFGWLGSLLMLFCLKSLYFKLQKFLAILLAVFEILVNLFSEGKIMSLLNIFIRSTMMLILAGNKVARFSFLNLEQKGGIY" }, "paralog": false, "identity": 0.979381443298969, "score": 182.0, "evalue": 5.12e-61, "description": "Internal stop codon at 14745. Nonsense mutation around 14745.", "psc": { "uniref90_id": "UniRef90_A0AB38W8J2", "uniref50_id": "UniRef50_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8J2", "UniRef:UniRef50_A0AB38W8J2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8J2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8148.923999999995, "isoelectric_point": 9.806624412536618 }, "id": "CNOCLNGIAL_63", "locus": "GCA000143945_00021" }, { "type": "cds", "sequence": "contig_1", "start": 14978, "stop": 15751, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_013", "db_xrefs": [ "RefSeq:WP_014325283.1", "SO:0001217", "UniParc:UPI0001DD6445", "UniRef:UniRef100_A0AAV5N6T9", "UniRef:UniRef50_P75100", "UniRef:UniRef90_P75100" ], "nt": "ATGCCTAAAGTTATTTCCCAAAAGGAATTTAATATCCTTTCCCGGACAAAGGAAAGAGTTAATTTTCAAATTGTCTATACCAAGCAAAGAAATGATTTGCACAAAGCTGCTAAGGAGTACTTTTTTAAAGACAAAGACTTTACAATAATAGAAGATACACCGGACACACCTGACCAACCAGATGGACCATGTATTACTAAAGGCCCAAAGGAACCAGGTGGTCCTGAAGAGCCAAATGGTCCGGATAAACCAGAAGATCCTAAAGATCCTGATACGCCAGATGTTTCTGAAGGCCCTAAAGAACAAGGTGGTCAGAAAGATCCAGGTGGTCCGGATGATCCCAATGAAGGAAAGCAGAAAGTGTCCAAACCAGATAAATACGTAACCCACCGTGAGCTTGATGAAAAGCTTAAAGACTTTGCCACCAAAGCTGATTTCAAACGCGTCGAAGACAAAGTTGATGTTTTATTTGAACTGCAAAAAACGCAAGGCGAACAAATAAAGGTTCAAGGTGAGCAGATCAAAGCTCAAGGAAAACAAATTGAACAATTAACTGAAACTGTCAAAGTGCAAGGCGAGCAGATTAGGGCTCAAGGAGAACAAATCAAAGCTCAGAGCGAAGAAATAAAAGAAATTAAGGTTGAGCAAAAAGCCCAAGGCGAGCAAATCAAAGAACTTCAAGTAGAACAAAAAGCCCAAGGTAAAACCCTACAGTTAATTTTAAAGACGCTAGAAAAAATGAACGAGCGTCTAGAAAAATTGGAATCTAAATAG", "aa": "MPKVISQKEFNILSRTKERVNFQIVYTKQRNDLHKAAKEYFFKDKDFTIIEDTPDTPDQPDGPCITKGPKEPGGPEEPNGPDKPEDPKDPDTPDVSEGPKEQGGQKDPGGPDDPNEGKQKVSKPDKYVTHRELDEKLKDFATKADFKRVEDKVDVLFELQKTQGEQIKVQGEQIKAQGKQIEQLTETVKVQGEQIRAQGEQIKAQSEEIKEIKVEQKAQGEQIKELQVEQKAQGKTLQLILKTLEKMNERLEKLESK", "aa_hexdigest": "ff5daef767ae27e0b646c6ca7ec8506d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6445", "ncbi_nrp_id": "WP_014325283.1", "uniref100_id": "UniRef100_A0AAV5N6T9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6445", "RefSeq:WP_014325283.1", "UniRef:UniRef100_A0AAV5N6T9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N6T9", "uniref90_id": "UniRef90_P75100" }, "psc": { "uniref90_id": "UniRef90_P75100", "product": "UPF0134 protein MPN_013", "uniref50_id": "UniRef50_P75100" }, "pscc": { "uniref50_id": "UniRef50_P75100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75100" ], "product": "UPF0134 protein MPN_013" }, "genes": [], "id": "CNOCLNGIAL_64", "locus": "GCA000143945_00022" }, { "type": "cds", "sequence": "contig_1", "start": 15853, "stop": 16425, "strand": "+", "frame": 1, "gene": "dnaG", "product": "putative MG010-like protein", "db_xrefs": [ "COG:COG0358", "COG:L", "GO:0003896", "GO:0005737", "SO:0001217", "UniRef:UniRef50_P75099", "UniRef:UniRef90_P75099" ], "nt": "ATGGAACTTAGAAATACAAAATTAGAACAACTCTTTTACATCTATCACCGCAACCTGAAGTATAGATGTCCAATGCAGTACCTCACTAAAAGGGGCTTTAACTTACAAGACCTCTTGTCAGTTGGTGGTGGATTAGCTTACTTGGGCGAAAAACAGTGGTTAAATTTATCACTGTACAACTTTAACAACCAGTTAGTTGGTTTTTTAAGTCGGAAGGTAGGTTTTGAAAAGAAGTTCCTTTATTTACCTATTAATAAACCACCAAGTAAAAGTGAATCTTTTTTAGGTTTAAAACACTTACCAACGGAAACGAACACCATTTACCTAGTTGAAGGTGATTTTGATTGGTTAGCTTTCCGTAAGGGTGGTATTTTGAACTGCTTGCCTTTATGTGGATTAACTTTATCAGATAAACAAATGAAGTTTTTTCAACAAAGTAAGATCGAAAAAGTGGTGCTTTGCTTAGACAATGATTTTGCTGGTAAAGTAGCTGCTGCTAACCTGGAACGCATTTTAAAAAATGCTGGTTTTCAGGTTAAGGTAGTTCAGCTAAAGGGTAAAGTTAAGGACTGA", "aa": "MELRNTKLEQLFYIYHRNLKYRCPMQYLTKRGFNLQDLLSVGGGLAYLGEKQWLNLSLYNFNNQLVGFLSRKVGFEKKFLYLPINKPPSKSESFLGLKHLPTETNTIYLVEGDFDWLAFRKGGILNCLPLCGLTLSDKQMKFFQQSKIEKVVLCLDNDFAGKVAAANLERILKNAGFQVKVVQLKGKVKD", "aa_hexdigest": "88f2bd83a79eaee97ec33021e7f80ae2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75099", "query_cov": 1.0, "subject_cov": 0.8962264150943396, "identity": 1.0, "score": 389.0, "evalue": 9.48e-137, "valid": true, "gene": "DnaG", "product": "Uncharacterized protein MG010 homolog", "uniref50_id": "UniRef50_P75099", "cog_id": "COG0358", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0003896" ] }, "pscc": { "uniref50_id": "UniRef50_P75099", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75099" ], "product": "Uncharacterized protein MG010 homolog" }, "genes": [ "dnaG" ], "id": "CNOCLNGIAL_65", "locus": "GCA000143945_00023" }, { "type": "cds", "sequence": "contig_1", "start": 16468, "stop": 16692, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCACAAAATTAAACGGATTGCTAAAAAGCTAGGTCATGGTTACTACGCCATTGACTTCTTCTTAAATAAGTACAACCGAATAGTGGTCAATGAAATCGAGGATGCTGCTGGTGCTAGGGCACTAACCAAAATGTGTCCTGACTTAAACCTACCAAGGGTAATTATTAAATCGTCCTTAACTCACTTTAAACATCATTTAAAGCGCCAAATGATCCCTTAA", "aa": "MKHKIKRIAKKLGHGYYAIDFFLNKYNRIVVNEIEDAAGARALTKMCPDLNLPRVIIKSSLTHFKHHLKRQMIP", "aa_hexdigest": "7e6ec999ceeedb8b58d445c113b12acb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8642.251299999996, "isoelectric_point": 10.201557731628416 }, "id": "CNOCLNGIAL_66", "locus": "GCA000143945_00024" }, { "type": "cds", "sequence": "contig_1", "start": 16696, "stop": 17325, "strand": "-", "frame": 3, "gene": "lysX", "product": "putative MG011-like protein", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47257", "UniRef:UniRef90_P75098" ], "nt": "ATGGCCAAAATTAAGTTAAAAAACAGAAAAGCACTCGTTGTTTACAATAAGACCGATTTTGACAAGAACAAGCACTTTGCTCAAGCACTAGTCGATGAACTGAACAAGAAAAAACTAGTTGGGCATATTTTGCTGTTAGATGATGAAACCGCTGATCACAAACACATTAAAAATGTGGAATTGATCATTAACCGTTCGCGCAGAATTGATTTTTTAACCAAACACAACTTTCTCAACAGTTTTCTCATTAATCCCCAAAATATCGTTTTGGTAGCCAATGATAAGTATGAAACTTACAGGTGGTTAAAGCAGCATAAGTTCTTGACCGTTGATACCACCATTTTTGATCCCAAAAAGATTAAGACTTTTCCGATTGTGATCAAAAAGCGTGATTCTCATGGTGGGGAAGATGTGCACTTAATTCAAAATGCGGAAGAAATTAAGCAGTTACCAATTCAAAATCCTAATGAGTGGATTGTCCAACCATTTTTGTCAATTGGTAAAGTGGAATACCGGGCTTACATCTTGTTTGGTAAGGTGTTAAAAACAATTAGAAGGACTGCTAGTGGTGATGATTTTAGGGCTAACTACTCACAAAATGCTGCTGTTGATTTGTTTAAACTAAAGTGA", "aa": "MAKIKLKNRKALVVYNKTDFDKNKHFAQALVDELNKKKLVGHILLLDDETADHKHIKNVELIINRSRRIDFLTKHNFLNSFLINPQNIVLVANDKYETYRWLKQHKFLTVDTTIFDPKKIKTFPIVIKKRDSHGGEDVHLIQNAEEIKQLPIQNPNEWIVQPFLSIGKVEYRAYILFGKVLKTIRRTASGDDFRANYSQNAAVDLFKLK", "aa_hexdigest": "e0883f97cc6f6a5e67c92068a48361f8", "start_type": "ATG", "rbs_motif": null, "genes": [ "lysX" ], "truncated": "5-prime", "pseudogene": { "start": 16468, "stop": 17325, "observations": { "mutation": [ 16698 ], "stop": [ 16698 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75098", "query_cov": 1.0, "subject_cov": 0.7333333333333333, "identity": 0.995, "score": 417.0, "evalue": 1.47e-146, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MAKIKLKNRKALVVYNKTDFDKNKHFAQALVDELNKKKLVGHILLLDDETADHKHIKNVELIINRSRRIDFLTKHNFLNSFLINPQNIVLVANDKYETYRWLKQHKFLTVDTTIFDPKKIKTFPIVIKKRDSYGGEDVHLIQNAEEIKQLPIQNPNEWIVQPFLSIGKVEYRAYILFGKVLKTIRRTASGDDFRANYSQNAAVDLFKLKWYIKHKIKRIAKKLGHGYYAIDFFLNKYNRIVVNEIEDAAGARALTKMCPDLNLPRVIIKSSLTHFKHHLKRQMIP" }, "paralog": false, "identity": 0.9894736842105263, "score": 567.0, "evalue": 5.7e-208, "description": "Internal stop codon at 16698. Nonsense mutation around 16698.", "psc": { "uniref90_id": "UniRef90_P75098", "gene": "LysX", "product": "Uncharacterized protein MG011 homolog", "uniref50_id": "UniRef50_P47257", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75098", "UniRef:UniRef50_P47257", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P47257", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47257" ], "product": "Uncharacterized protein MG011" } }, "id": "CNOCLNGIAL_67", "locus": "GCA000143945_00025" }, { "type": "cds", "sequence": "contig_1", "start": 17325, "stop": 17813, "strand": "-", "frame": 1, "gene": "lysX", "product": "putative MG012-like protein", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0036211", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75097", "UniRef:UniRef90_P75097" ], "nt": "TTGGAACAATTCATCGAACAAATTAAGCGCTGTTTTCAGTTTCCAGTGATTGTCAAAAGTATCTATGGTTCTTTTGGTGATTACGTTTTTTTGTGCAAAGATGAACAGAAGTTACGTCAAACCTTAAGCGGGCTAACTGAACAGATCATTGTGCAGCAATACATTGCCACTTCTAATAGTGAAGCGGTCAGGGTAATTGTGGTCAATAACCAGGTAGTTGGGGCCTTACATACTCAAAATGAGGGTGATTTTCGCTCTAATTTAAATAAGGGAGCGGTTGGTGAACCATATCAACTAAGTCAGGAAGAAACCAAATTAGCAATTACAATTAGCCAAGCAATGCAACTCTTTTATTGTGGCATTGACTTTTTATTTGACCAGGATCGTTCGTTAATTTTCTGCGAAGTTAATTCCAATGTACAGCTCACCAAAAGCTCGATGTATTTGAAAACTAACCTCGCCATTCAACTTTTAGCATCAATCGCATAA", "aa": "MEQFIEQIKRCFQFPVIVKSIYGSFGDYVFLCKDEQKLRQTLSGLTEQIIVQQYIATSNSEAVRVIVVNNQVVGALHTQNEGDFRSNLNKGAVGEPYQLSQEETKLAITISQAMQLFYCGIDFLFDQDRSLIFCEVNSNVQLTKSSMYLKTNLAIQLLASIA", "aa_hexdigest": "da3b53ab4ef40bccb9279677950a0a35", "start_type": "TTG", "rbs_motif": null, "genes": [ "lysX" ], "truncated": "3-prime", "pseudogene": { "start": 17325, "stop": 18113, "observations": { "mutation": [ 17828 ], "stop": [ 17828 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75097", "query_cov": 1.0, "subject_cov": 0.5625, "identity": 0.9940000000000001, "score": 313.0, "evalue": 3.07e-106, "gene_start": 1, "gene_end": 162, "reference_start": 127, "reference_end": 288, "reference_sequence": "MKHISAVYNPAFTNIASKLNQTELLKDAAQSLNIQLDFFTCFDINTNQDKTKLPFKSNTILFLDKNIALAQWLESVGLRVINSSIAINNADNKALSHAVLAQHPTIKQIPTLIGPQNFRLAWYPEKLEQFIEQIKRCFQFPVIVKSIYGSFGDYVFLCKDEQKLRQTLSGLTEQIIVQQYIATSNSEAVRVIVVNNQVVGALHTQNEGDFRSNLNKGAVGEPYQLSQEETKLAITISQAMQLFYCGIDFLFDQDRSLIFCEVNSNVQLTKSSMYLKTNLAIQLLASIA" }, "paralog": false, "identity": 0.9961832061068703, "score": 510.0, "evalue": 3.55e-186, "description": "Internal stop codon at 17828. Nonsense mutation around 17828.", "psc": { "uniref90_id": "UniRef90_P75097", "gene": "LysX", "product": "Uncharacterized protein MG012 homolog", "uniref50_id": "UniRef50_P75097", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75097", "UniRef:UniRef50_P75097", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75097", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75097" ], "product": "Uncharacterized protein MG012 homolog" } }, "id": "CNOCLNGIAL_68", "locus": "GCA000143945_00026" }, { "type": "cds", "sequence": "contig_1", "start": 17826, "stop": 18191, "strand": "-", "frame": 1, "gene": "lysX", "product": "putative MG012-like protein", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0036211", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75097", "UniRef:UniRef90_P75097" ], "nt": "ATGAAGCACATCAGCGCCGTATATAACCCTGCTTTTACTAACATTGCTTCCAAATTAAACCAAACGGAACTGTTAAAGGATGCAGCCCAGAGCTTAAACATCCAACTCGATTTCTTTACTTGCTTTGATATTAATACCAATCAGGATAAGACCAAACTACCCTTTAAGTCCAATACCATTTTGTTTTTGGATAAAAACATAGCGTTAGCGCAATGGTTAGAATCAGTTGGTTTGCGCGTTATTAACAGTTCGATTGCCATTAATAACGCGGACAATAAGGCCTTGAGTCATGCTGTTTTAGCGCAACACCCAACCATTAAACAGATTCCTACATTAATTGGCCCACAGAACTTTCGTTTAGCTTGA", "aa": "MKHISAVYNPAFTNIASKLNQTELLKDAAQSLNIQLDFFTCFDINTNQDKTKLPFKSNTILFLDKNIALAQWLESVGLRVINSSIAINNADNKALSHAVLAQHPTIKQIPTLIGPQNFRLA", "aa_hexdigest": "e3eadba98d3468ed554dbc7a60e2d616", "start_type": "ATG", "rbs_motif": "AAAAA", "genes": [ "lysX" ], "truncated": "5-prime", "pseudogene": { "start": 17523, "stop": 18191, "observations": { "mutation": [ 17828 ], "stop": [ 17828 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75097", "query_cov": 1.0, "subject_cov": 0.4201388888888889, "identity": 1.0, "score": 241.0, "evalue": 3e-78, "gene_start": 1, "gene_end": 121, "reference_start": 1, "reference_end": 121, "reference_sequence": "MKHISAVYNPAFTNIASKLNQTELLKDAAQSLNIQLDFFTCFDINTNQDKTKLPFKSNTILFLDKNIALAQWLESVGLRVINSSIAINNADNKALSHAVLAQHPTIKQIPTLIGPQNFRLAWYPEKLEQFIEQIKRCFQFPVIVKSIYGSFGDYVFLCKDEQKLRQTLSGLTEQIIVQQYIATSNSEAVRVIVVNNQVVGALHTQNEGDFRSNLNKGAVGEPYQLSQEETKLAITISQAMQLFYCGIDFLFDQDRSLIFCEVNSNVQLTKSSMYLKTNLAIQLLASIA" }, "paralog": false, "identity": 0.9954954954954955, "score": 435.0, "evalue": 3.48e-157, "description": "Internal stop codon at 17828. Nonsense mutation around 17828.", "psc": { "uniref90_id": "UniRef90_P75097", "gene": "LysX", "product": "Uncharacterized protein MG012 homolog", "uniref50_id": "UniRef50_P75097", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75097", "UniRef:UniRef50_P75097", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75097", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75097" ], "product": "Uncharacterized protein MG012 homolog" } }, "id": "CNOCLNGIAL_69", "locus": "GCA000143945_00027" }, { "type": "cds", "sequence": "contig_1", "start": 18259, "stop": 18873, "strand": "-", "frame": 3, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "GO:0000105", "GO:0004477", "GO:0004488", "GO:0005829", "GO:0006164", "GO:0009086", "GO:0035999", "SO:0001217", "UniRef:UniRef50_P47259", "UniRef:UniRef90_P75096" ], "nt": "GTGTCATCTGATGCCTTTATTCGCCAAAAAATGAATGCTTGTAATACTCTAGGGGCCCAGAGTGAACTAATTAAGTACCACGAAAGTGTCACTGAGAAAGAATTGTTAGAAAAAATTCAACAACTCAACACTGATCCTGAGGTAACAGGGATTATCTTGCAACTGCCAGTGTATCCCCATTTAAAACAAAACAAGCTCTTAAAAGCAATTGATCCTTACAAGGATGTGGATTACTTAACTGGTAACTGCCCCCTACCCATTAGGTCCTGTGTGGTGGAAGCGGTGTTAATTTTGAAAGAACACTTTCACCATGATTTCACCAACAAGCAGATTGTGGTGGTGGGTTTAGGCGTCACTGGTGGCGGGCCGATCTTTCACTATTTAAAGGAAACGGGTCATCAGGTGGTGGGATGTGACAAGCATACCCCCAACACCATGGAGCTAATTAAAACCGCTGACGTGGTGATCACAGCCATTGGCAAGCCCCATTTCTTTAAGACCACTAACTTTAAACCAGGTGTTATTCTTTATGATGTGGGGGTATCACGTAATGCCTTAAACAAACTGTGTGGTGACATCGATCCTAATGGGATAGAGAAAGTTGCCAAATGGTGA", "aa": "MSSDAFIRQKMNACNTLGAQSELIKYHESVTEKELLEKIQQLNTDPEVTGIILQLPVYPHLKQNKLLKAIDPYKDVDYLTGNCPLPIRSCVVEAVLILKEHFHHDFTNKQIVVVGLGVTGGGPIFHYLKETGHQVVGCDKHTPNTMELIKTADVVITAIGKPHFFKTTNFKPGVILYDVGVSRNALNKLCGDIDPNGIEKVAKW", "aa_hexdigest": "e7056c48dbf7c84d900d48e48921404d", "start_type": "GTG", "rbs_motif": null, "genes": [ "folD" ], "truncated": "both", "pseudogene": { "start": 18166, "stop": 18975, "observations": { "mutation": [ 18201, 18261, 18915 ], "stop": [ 18201, 18261, 18915 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75096", "query_cov": 1.0, "subject_cov": 0.758364312267658, "identity": 0.99, "score": 416.0, "evalue": 2.76e-146, "gene_start": 1, "gene_end": 204, "reference_start": 35, "reference_end": 238, "reference_sequence": "MSFDGKQLAHEILLTYKNRDWSQVKLVILQTNNDVSSDAFIRQKMNACNTLGAQSELIKYHESVTEKELLEKIQQLNTDPEVTGIILQLPVYPHLKQNKLLKAIDPYKDVDYLTGNCPLPIRSCVVEAVLILKEHFHHDFTNKQIVVVGLGVTGGGPIFHYLKETGHQVVGCDKHTPNTMELIKTADVVITAIGKPHFFKTTNFKPGVILYDVGVSRNVLNKLCGDIDPNGIEKVAKWWSPTPGGVGPFTVLAIMKNLWVLYEAHQRRI" }, "paralog": false, "identity": 0.9851301115241635, "score": 536.0, "evalue": 7.96e-196, "description": "Internal stop codon at 18201, 18261, 18915. Nonsense mutation around 18201, 18261, 18915.", "psc": { "uniref90_id": "UniRef90_P75096", "gene": "folD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_P47259", "cog_id": "COG0190", "cog_category": "H", "go_ids": [ "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75096", "UniRef:UniRef50_P47259", "COG:COG0190", "COG:H", "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_P47259", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47259" ], "product": "Bifunctional protein FolD" } }, "id": "CNOCLNGIAL_70", "locus": "GCA000143945_00028" }, { "type": "tRNA", "sequence": "contig_1", "start": 19060, "stop": 19135, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 68.8, "nt": "GGGGATGTAGCTCAACTGATAGAGCACCTGATTTGCACTCAGGAGGTTGAGGGTTTGAGACCCTTCATCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 19100, 19102 ], "id": "CNOCLNGIAL_36", "locus": "GCA000143945_00029" }, { "type": "tRNA", "sequence": "contig_1", "start": 19141, "stop": 19217, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 84.3, "nt": "GGAAGTATAGCTCAGTTGGTTAGAGCACACCCCTGATAAGGGTGAGGTCGATGGTTCAAGTCCATTTACTTCCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 19181, 19183 ], "id": "CNOCLNGIAL_35", "locus": "GCA000143945_00030" }, { "type": "cds", "sequence": "contig_1", "start": 19311, "stop": 20066, "strand": "+", "frame": 3, "gene": "mdlB", "product": "Putative ABC transporter ATP-binding MG014-like protein", "db_xrefs": [ "COG:COG1132", "COG:V", "GO:0005524", "GO:0005886", "GO:0015421", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47260", "UniRef:UniRef90_P75095" ], "nt": "ATGGGTCTTGTTTTAAAACAATTTAACAGGAAAATAAGAACAGCTTTAATTCTGGCGCCGCTTTTCACCTTTGCACAGATTATTATTGATCTTATTATTCCGTCCTTTTTGGCCAGTGCTATTGCCGTAGTGTTCTCAATTGTCACGCTTAAACAAAAAGAAGCATCAGGCGAGGGAGTTGCTGTTGATTTTATTGCTGAAAGTAAGTTAAGTTTCCAAAGTGTCCAGGAAGCGCAAATAGTGTTAGCGACCTCAGTGATCTTGTTAGCACTCTTTGGTTTAGTGTTTGGGTTAATTTCCATTTTTTGTGCCAGCATAGTAGCGGGGAATACCTCTTACTTTTTAAGAAGGAAGATTTTTCGCAAGATTATGCACATTACCGCACCTTCTCATGACCAATATGGCTCTTCTACGTTGTTGGTAAGGTTGACCAATGACGTTTACTTAATGGAAATCATGACCTTTGATTTTTTGCGGTTAATTGTTCGTGCACCTTTCTTGTTTATAGGGGGTCTAGCCTTTGCTATTGCCACTAACTCTGACATGTCGATTTCGCTAGCCATTACCTTTCCTTTGACCTTCTTAGTGATTGGGATTCTCAACAAGAAATCGACCCCGTTATTTAAGAACAACCAGAAATCGGTGGACCAAATTAACGAACGCGTGGAGGAAGATGTGTCGGGTTACAAGGTAGTGCAGTCGTTTAACTTAAAGGACACGGAATGTCTCAAGTTTAAGGCAGCTAATGCAAGGTGA", "aa": "MGLVLKQFNRKIRTALILAPLFTFAQIIIDLIIPSFLASAIAVVFSIVTLKQKEASGEGVAVDFIAESKLSFQSVQEAQIVLATSVILLALFGLVFGLISIFCASIVAGNTSYFLRRKIFRKIMHITAPSHDQYGSSTLLVRLTNDVYLMEIMTFDFLRLIVRAPFLFIGGLAFAIATNSDMSISLAITFPLTFLVIGILNKKSTPLFKNNQKSVDQINERVEEDVSGYKVVQSFNLKDTECLKFKAANAR", "aa_hexdigest": "b14ef64c41b6695270d5f377985cf1b4", "start_type": "ATG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "3-prime", "pseudogene": { "start": 19311, "stop": 20369, "observations": { "mutation": [ 20064 ], "stop": [ 20064 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75095", "query_cov": 1.0, "subject_cov": 0.4028892455858748, "identity": 1.0, "score": 454.0, "evalue": 1.78e-155, "gene_start": 1, "gene_end": 251, "reference_start": 1, "reference_end": 251, "reference_sequence": "MGLVLKQFNRKIRTALILAPLFTFAQIIIDLIIPSFLASAIAVVFSIVTLKQKEASGEGVAVDFIAESKLSFQSVQEAQIVLATSVILLALFGLVFGLISIFCASIVAGNTSYFLRRKIFRKIMHITAPSHDQYGSSTLLVRLTNDVYLMEIMTFDFLRLIVRAPFLFIGGLAFAIATNSDMSISLAITFPLTFLVIGILNKKSTPLFKNNQKSVDQINERVEEDVSGYKVVQSFNLKDTECLKFKAANARWTKSSTNSLFVNTLNIPFTFFFSSMTIIIALLLVFQLDNSVRVDPLPDNAAIRPSIFAFFQYNFYIVLGLILTSLTMVNFTRSRVALGRIKDVLNKPEIQQHVVPDQTVLAPSLEFKNVAFGLGNKENRDFLQDLNFKFEAGKTYGIVGPTGSGKSLIANIIGGLYEPNQGEIFVGGQSIKTIDSDYLAKMIGIVFQQNILFKGTIASNIKIGLETREDWKHEPDSKKDAAMKRAAAIACADTFIEKFSDTYDHTVEQLGKNLSGGQKQRVAIARTVITKPQILVLDDSMSALDALTEKKVRENIANELPGTTKIIISQNINSIKYAHKIMVIDNGRIAGFDSDAKLMQSCDIYVKMKQAQKDQGGDFDAVA" }, "paralog": false, "identity": 0.9971590909090909, "score": 662.0, "evalue": 1.59e-239, "description": "Internal stop codon at 20064. Nonsense mutation around 20064.", "psc": { "uniref90_id": "UniRef90_P75095", "gene": "MdlB", "product": "Putative ABC transporter ATP-binding protein MG014 homolog", "uniref50_id": "UniRef50_P47260", "cog_id": "COG1132", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75095", "UniRef:UniRef50_P47260", "COG:COG1132", "COG:V", "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47260", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47260" ], "product": "Putative ABC transporter ATP-binding protein MG014" } }, "id": "CNOCLNGIAL_71", "locus": "GCA000143945_00031" }, { "type": "cds", "sequence": "contig_1", "start": 20088, "stop": 21182, "strand": "+", "frame": 3, "gene": "mdlB", "product": "Putative ABC transporter ATP-binding MG014-like protein", "db_xrefs": [ "COG:COG1132", "COG:V", "GO:0005524", "GO:0005886", "GO:0015421", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47260", "UniRef:UniRef90_P75095" ], "nt": "TTGTTTGTCAATACACTGAACATTCCCTTTACCTTCTTCTTTTCCAGTATGACTATCATCATTGCGTTGCTTTTAGTCTTCCAACTAGATAATTCAGTCAGAGTGGATCCCCTACCAGATAATGCTGCCATTAGACCCAGCATCTTTGCTTTCTTCCAGTACAACTTCTATATTGTCTTGGGTTTAATCTTGACCTCGCTAACAATGGTCAACTTTACCCGTTCCCGCGTGGCCTTGGGTCGGATTAAGGATGTTTTGAATAAACCGGAAATCCAACAACATGTGGTTCCTGATCAAACAGTTCTAGCCCCTAGCTTGGAATTCAAAAACGTGGCCTTTGGGTTAGGTAACAAGGAAAACCGGGACTTCTTGCAAGACCTTAACTTTAAGTTTGAGGCCGGTAAGACTTACGGAATTGTGGGACCTACTGGTTCGGGTAAGTCTTTAATAGCTAATATTATTGGGGGTTTATATGAACCCAACCAAGGTGAAATCTTTGTTGGTGGTCAAAGTATTAAAACGATTGACAGTGACTATTTAGCCAAGATGATCGGGATTGTCTTTCAGCAAAACATTCTCTTTAAGGGCACGATTGCTTCCAACATTAAGATAGGCCTGGAAACCAGGGAAGACTGGAAGCACGAACCAGATAGCAAAAAGGATGCTGCCATGAAACGCGCTGCTGCCATTGCTTGTGCTGATACCTTCATCGAAAAGTTCAGCGATACCTATGACCATACGGTGGAACAGTTAGGCAAAAACCTCTCTGGTGGGCAAAAACAACGCGTCGCCATTACTAGAACCGTTATTACTAAACCACAAATCTTAGTGTTGGATGACAGTATGAGTGCGCTGGACGCTTTAACCGAAAAGAAGGTCCGGGAAAACATTGCCAACGAACTTCCGGGCACCACTAAGATCATTATTAGTCAAAACATTAATTCGATTAAGTACGCGCACAAAATTATGGTGATTGATAACGGTCGGATTGCTGGGTTTGACAGTGATGCTAAGCTCATGCAGAGCTGTGACATCTATGTCAAGATGAAGCAAGCACAAAAAGACCAAGGAGGTGATTTCGATGCTGTCGCATAA", "aa": "MFVNTLNIPFTFFFSSMTIIIALLLVFQLDNSVRVDPLPDNAAIRPSIFAFFQYNFYIVLGLILTSLTMVNFTRSRVALGRIKDVLNKPEIQQHVVPDQTVLAPSLEFKNVAFGLGNKENRDFLQDLNFKFEAGKTYGIVGPTGSGKSLIANIIGGLYEPNQGEIFVGGQSIKTIDSDYLAKMIGIVFQQNILFKGTIASNIKIGLETREDWKHEPDSKKDAAMKRAAAIACADTFIEKFSDTYDHTVEQLGKNLSGGQKQRVAITRTVITKPQILVLDDSMSALDALTEKKVRENIANELPGTTKIIISQNINSIKYAHKIMVIDNGRIAGFDSDAKLMQSCDIYVKMKQAQKDQGGDFDAVA", "aa_hexdigest": "a60b99f2f5cf40b827408b54fea6b089", "start_type": "TTG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "5-prime", "pseudogene": { "start": 19788, "stop": 21182, "observations": { "mutation": [ 20064 ], "stop": [ 20064 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75095", "query_cov": 1.0, "subject_cov": 0.5842696629213483, "identity": 0.995, "score": 702.0, "evalue": 3.16e-251, "gene_start": 1, "gene_end": 364, "reference_start": 260, "reference_end": 623, "reference_sequence": "MGLVLKQFNRKIRTALILAPLFTFAQIIIDLIIPSFLASAIAVVFSIVTLKQKEASGEGVAVDFIAESKLSFQSVQEAQIVLATSVILLALFGLVFGLISIFCASIVAGNTSYFLRRKIFRKIMHITAPSHDQYGSSTLLVRLTNDVYLMEIMTFDFLRLIVRAPFLFIGGLAFAIATNSDMSISLAITFPLTFLVIGILNKKSTPLFKNNQKSVDQINERVEEDVSGYKVVQSFNLKDTECLKFKAANARWTKSSTNSLFVNTLNIPFTFFFSSMTIIIALLLVFQLDNSVRVDPLPDNAAIRPSIFAFFQYNFYIVLGLILTSLTMVNFTRSRVALGRIKDVLNKPEIQQHVVPDQTVLAPSLEFKNVAFGLGNKENRDFLQDLNFKFEAGKTYGIVGPTGSGKSLIANIIGGLYEPNQGEIFVGGQSIKTIDSDYLAKMIGIVFQQNILFKGTIASNIKIGLETREDWKHEPDSKKDAAMKRAAAIACADTFIEKFSDTYDHTVEQLGKNLSGGQKQRVAIARTVITKPQILVLDDSMSALDALTEKKVRENIANELPGTTKIIISQNINSIKYAHKIMVIDNGRIAGFDSDAKLMQSCDIYVKMKQAQKDQGGDFDAVA" }, "paralog": false, "identity": 0.9956896551724138, "score": 897.0, "evalue": 0.0, "description": "Internal stop codon at 20064. Nonsense mutation around 20064.", "psc": { "uniref90_id": "UniRef90_P75095", "gene": "MdlB", "product": "Putative ABC transporter ATP-binding protein MG014 homolog", "uniref50_id": "UniRef50_P47260", "cog_id": "COG1132", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75095", "UniRef:UniRef50_P47260", "COG:COG1132", "COG:V", "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47260", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47260" ], "product": "Putative ABC transporter ATP-binding protein MG014" } }, "id": "CNOCLNGIAL_72", "locus": "GCA000143945_00032" }, { "type": "cds", "sequence": "contig_1", "start": 21229, "stop": 21540, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGGTCGCACTTCCTCCAAGGTGCTGTATGTCATGGTCTGTGCCATCTTTGGTATTCTCACTGGGGTTACTAACTCGATTCTCTTGGCCCAGGGCCTGGGTTTTATTTTTCCTACCACTAATACCGAAACTGATGGTATCCAATCAGTGTACTTACTGGTATTTGCGCACAACTTACCGGTCATGGAGCGCTTGACGATTGTTTGTGTTACCGTGGTTGTGGCTTACATTCTCATCTTTAGTTTTAACGTAGCGCAGAACTATTTAGGTTTAAAGCTGTACCAAGAGATCTGTGCCTTGTTGCGCTGA", "aa": "MQGRTSSKVLYVMVCAIFGILTGVTNSILLAQGLGFIFPTTNTETDGIQSVYLLVFAHNLPVMERLTIVCVTVVVAYILIFSFNVAQNYLGLKLYQEICALLR", "aa_hexdigest": "31c83c977d03b970aba79cd36d9158f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11365.43409999999, "isoelectric_point": 7.711539268493653 }, "id": "CNOCLNGIAL_73", "locus": "GCA000143945_00033" }, { "type": "cds", "sequence": "contig_1", "start": 21565, "stop": 22170, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCACTAGTTTCTTTGATACGCAGAACAACGGGGATTTAATGAGTCGCTTGACCAACGACGTTTACAACATTAACAACCTGTATGCCCAAGTGGGTGGACAGACAATTCAGAGTTTGTTTATCTTAATGACAACAGCGACGATTCTGTTTGTTTTAAGTCCAGTCATTGCCTTAATTTCGCTAACAGTCTTAATTGCTCTAATTGCCCTTTCCTTTCTCTTTTTAAAGAAGGCACGCGCTGCTTATGCAAAGGTGCAAAACAACTTAGGGGACATGTCGGGTTACATTGAAGAAGTGTTGTCGAACCATAAGGTAGTTCATGTCTTGAAACTGCAAGAGGTGATGATTGATAACTTTGACAAGTACAACCGCCCGATGGTTAACCCCACCATTAAGGCCAATACCTATGCGGTGTTTATTTACTCCTGGTTTGGTTTTATTTCCAATATTACTTACTTAGCTTCGATCTCAATTGCCACGGCCTTTAGTGTGAATAACATTCCCTCCTTTGGGGTGAGTGCGATTAACTATTCGTTTATGCTGTCTTACATTGCGGCCTTACGTCAAACAGCACTCCCTTTAAACCAGATCTTTAGCTTGTGA", "aa": "MSTSFFDTQNNGDLMSRLTNDVYNINNLYAQVGGQTIQSLFILMTTATILFVLSPVIALISLTVLIALIALSFLFLKKARAAYAKVQNNLGDMSGYIEEVLSNHKVVHVLKLQEVMIDNFDKYNRPMVNPTIKANTYAVFIYSWFGFISNITYLASISIATAFSVNNIPSFGVSAINYSFMLSYIAALRQTALPLNQIFSL", "aa_hexdigest": "cbb029256f4e08b4f3171f348f58c13d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22366.792800000003, "isoelectric_point": 8.803302955627444 }, "id": "CNOCLNGIAL_74", "locus": "GCA000143945_00034" }, { "type": "cds", "sequence": "contig_1", "start": 22219, "stop": 22500, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGGATCTAGAAAGTCCCCAAAAACAGGCCACCATTACTAAGTTACCCAACATTAAGGGCAACATCCGTTTTGAAAAGGTAGCTTTTGGTTACAGTGCGGACAAACCAATTTTAACGGGGATTGATTTCTCGGTTAAACACGGTGACATTGTGGCCATAGTCGGTCCTACTGGAGCGGGTAAGTCCACCATTATTAACCTGTTGATGAAGTTCTACAAACCGTTTGCTGGGAAGATCTACATGGACAACTTTGAGATTAGTGAGGTGAGTGAAACTGCTTGA", "aa": "MDLESPQKQATITKLPNIKGNIRFEKVAFGYSADKPILTGIDFSVKHGDIVAIVGPTGAGKSTIINLLMKFYKPFAGKIYMDNFEISEVSETA", "aa_hexdigest": "b9822d01461eb319aa53df4a7546dea7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 51, "aa_cov": 0.5483870967741935, "hmm_cov": 1.0, "evalue": 2.4638801124586492e-14, "score": 49.54983139038086, "start": 38, "stop": 88 } ], "seq_stats": { "molecular_weight": 10167.684999999998, "isoelectric_point": 7.947614097595215 }, "id": "CNOCLNGIAL_75", "locus": "GCA000143945_00035" }, { "type": "cds", "sequence": "contig_1", "start": 22519, "stop": 22998, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGCTCCAAGACCCCTTCTTGTTTAGTGGCACGATTAAGGAAAACATCCGCATGGGACGGCAAGATGCCACTGATGAGGAAATTATAGAGGCGTGCAAGGTGGCCAATGCTCATGACTTTATTATGCGTTTACCCCAAGGTTACAACACCTTTATTTCCAACAAAACTGATTATCTCTCGGTCGGTGAGCGCCAACTGTTAACTATTGCCCGCGCTGTTATTCGTAATGCCCCGGTGTTATTATTGGATGAAGCCACGAGCTCCATCGATGTACACTCCGAAAAATTGATCCAACAATCAATAGGCAGACTAATGAAGGATAAAACATCGTTTATAATATCCCATCGACTCTCAATCATCCGTAATGCGACGCTGATAATTGTGATTAATGATGGAAAAGTTTTGGAAATGGGTAACCATGAACAATTAATGCGGCAAAACGGTTTTTATGCTCGATTAAAGCGCTCTGCTGTGAAGTAA", "aa": "MLQDPFLFSGTIKENIRMGRQDATDEEIIEACKVANAHDFIMRLPQGYNTFISNKTDYLSVGERQLLTIARAVIRNAPVLLLDEATSSIDVHSEKLIQQSIGRLMKDKTSFIISHRLSIIRNATLIIVINDGKVLEMGNHEQLMRQNGFYARLKRSAVK", "aa_hexdigest": "eec678936ab4730ac6829e1efe0efd98", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00307619A1", "query_cov": 0.9811320754716981, "subject_cov": 0.8082901554404145, "identity": 0.551, "score": 176.0, "evalue": 3.12e-53, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A354MUK2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 88, "aa_cov": 0.5345911949685535, "hmm_cov": 0.8181818181818182, "evalue": 1.5622950596755653e-11, "score": 40.47453308105469, "start": 3, "stop": 87 } ], "seq_stats": { "molecular_weight": 18043.70329999999, "isoelectric_point": 9.12822360992432 }, "id": "CNOCLNGIAL_76", "locus": "GCA000143945_00036" }, { "type": "cds", "sequence": "contig_1", "start": 23008, "stop": 23382, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04434.22" ], "nt": "ATGACGATTGCTGAAATAAGGAAATTTGCACAAACCACGCAAAAGTTTGTCGAAGCGGAAAACCTGTTTGAGCATGGTAACGTAGTCTTGCCCAAAAAATACCTCAATAAGGCGCGGGGCATGGCTGAAGTATTGTACAACAGCCAGGTCATTAAAGTAAGTTTTACGGCTAAAGATGGTGAGCTAACGTGTAAGTGTAGTTGTTTGGCTAACGTTGACAACTGCGTGCACATTGTAGCTGTATTGCTCAAATACCACCAAATGCTAGTGGAGTCCAAACGCAGTTTTAACTTAGCTGAAGCCTTTCACTTGGACTGTGATCAGGCCGAAATGCTGATTGAAAACCTCAGTTTAGAGATTATTGCCGGGGGATGA", "aa": "MTIAEIRKFAQTTQKFVEAENLFEHGNVVLPKKYLNKARGMAEVLYNSQVIKVSFTAKDGELTCKCSCLANVDNCVHIVAVLLKYHQMLVESKRSFNLAEAFHLDCDQAEMLIENLSLEIIAGG", "aa_hexdigest": "1c9ab90bbbc57fb82fa825fca0a254e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04434.22", "name": "SWIM", "length": 29, "aa_cov": 0.20967741935483872, "hmm_cov": 1.0, "evalue": 6.223794363379662e-08, "score": 27.563405990600586, "start": 60, "stop": 85 } ], "seq_stats": { "molecular_weight": 13894.024299999988, "isoelectric_point": 6.063549613952636 }, "id": "CNOCLNGIAL_77", "locus": "GCA000143945_00037" }, { "type": "cds", "sequence": "contig_1", "start": 23440, "stop": 24540, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCTTTTATTGTTGTGATGCGACCTTTGTGTACTTCCTTCACTTGGAAAACGACACTTTGCACTCAGTCGAATTGTCCAAGTTTAAACCAGAGGAACGCGCTACCTTACTCTTCTTTGATAAATTGTGCAAGCAGTTTACAGTGGGGTATGACCGCAACAGTTTGCTCTTTCCGTTAGCGGGTTTTTTAAAGGAGTTACAAGCCAACACGGAGCCCACCATCTTTGTCTTTAACGATGACAAAATTGACAACATCCTCTTTTTAAGGATTTCCAAAAAGCACCATGGTTTAAACCATGTTTGTGGTTTTAGTGGCAAGAAGGTATTTGACTTTGTTACTTATAAACAAAAGGAAAAGCAGATTGTGTTGCGCTCAGCGTACCTGAGTAAGTTCACTGACTTTAAGTTTCCTTACACCATTAACATCTACAAACTCCAGTTTGGGGAACCGCTGTTCTTTCACTTTTTAATTCAGCTAAAGCGTGATGGCTTTAAGAACTTCTACTTTCAAAGTGAAGACAGTATTGTCAAGGAAAAGGAATACCTACCGAAGCTGTACTTTAAGGTCGAGTACGATCCGGTCAAAAACAAGTTTGTCAGTGATGCCTTCTTTAAGTACAAGAACCACTTTAATAAGGGCACAACCACGGTGTATCCGCACCGCTATTACATGGCTAAAAAGTCGGATCGCGGTGGTTTTAACCGCTTGTTGTTTTACGAAGAGGCCGTGGAAAACTTCTACCAAGACCAGTTTGATTTGGGTTACTTCCGTAAGTTTGAACACCTACCGATCCAGGACAAAAACCAGATTGAGGCCTTTAAAGCAGCGCTAGATGACTTAATGCCCGTGGATTTGGCTGAAGTGAGTTTATCCGATAACTTACTCCACCAAAAACCGCTTCATTTTGCCCTGTCTGATCTAGAAGCAGTTGCTGTTGATGACAAACAAATTAAGTTAAGTTTTGCCCCTAGTGCGGTTGAACTGAAATTAATTAAACGGATCTTATCCGCTTACCATAAGGGCAATGTGGTCTGTATCGACCAGGAGAGTTGGTATGACCTCAAGCAACCAGCTGCTAAAGAACTAATCCAGTTCTGA", "aa": "MRFYCCDATFVYFLHLENDTLHSVELSKFKPEERATLLFFDKLCKQFTVGYDRNSLLFPLAGFLKELQANTEPTIFVFNDDKIDNILFLRISKKHHGLNHVCGFSGKKVFDFVTYKQKEKQIVLRSAYLSKFTDFKFPYTINIYKLQFGEPLFFHFLIQLKRDGFKNFYFQSEDSIVKEKEYLPKLYFKVEYDPVKNKFVSDAFFKYKNHFNKGTTTVYPHRYYMAKKSDRGGFNRLLFYEEAVENFYQDQFDLGYFRKFEHLPIQDKNQIEAFKAALDDLMPVDLAEVSLSDNLLHQKPLHFALSDLEAVAVDDKQIKLSFAPSAVELKLIKRILSAYHKGNVVCIDQESWYDLKQPAAKELIQF", "aa_hexdigest": "8a4437a313ec6a2557d743580948e873", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 43257.24040000007, "isoelectric_point": 8.32223987579346 }, "id": "CNOCLNGIAL_78", "locus": "GCA000143945_00038" }, { "type": "cds", "sequence": "contig_1", "start": 24613, "stop": 24771, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCAAGATCTTTAGTCAGTACTTGGACATTAAAAACCTGTTTGATGTACCGACCATTAAAAAGATTGAAGACCAAAACAACAACGTCTTTGACCTTTCCTTAGAACACAAAAAGATTACTTCACTGCGCAACTACCAACAAGAAGGAGTGAAGTGA", "aa": "MAKIFSQYLDIKNLFDVPTIKKIEDQNNNVFDLSLEHKKITSLRNYQQEGVK", "aa_hexdigest": "7fcdafb0b2a1e95f22e7e76ad5f862b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6155.9847, "isoelectric_point": 8.029811286926268 }, "id": "CNOCLNGIAL_79", "locus": "GCA000143945_00039" }, { "type": "cds", "sequence": "contig_1", "start": 24781, "stop": 24933, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00176.29" ], "nt": "TTGGAGGAAAATAAGTTTGGTGGTATTCTGGCTGACGAAATGGGGTTAGGGAAAACGGTGCAGGTGATCTTTGCGCTGTTGGACAGCTATCTCAAAAACCACGTTAACCTCCCTAGCTTAATTATTGTTCCGGCTTCTTTATTGTTGAACTGA", "aa": "MEENKFGGILADEMGLGKTVQVIFALLDSYLKNHVNLPSLIIVPASLLLN", "aa_hexdigest": "06aeeb07d933bf4c611fa98f18b6cd66", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 53, "aa_cov": 0.96, "hmm_cov": 0.9811320754716981, "evalue": 3.725772405517927e-13, "score": 44.63841247558594, "start": 2, "stop": 49 } ], "seq_stats": { "molecular_weight": 5440.376199999999, "isoelectric_point": 4.895849418640137 }, "id": "CNOCLNGIAL_80", "locus": "GCA000143945_00040" }, { "type": "cds", "sequence": "contig_1", "start": 25009, "stop": 25233, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00176.29" ], "nt": "TTGTACGAAAAACTTACTAACGAAATCCTGATTGTGAGCTTTAACGTGTTGCGCAGTGATGTGAAGTTAATTACCAAACAGCGCTTTCACTATGTTGTAATTGACGAAGCGCAGGGCATTAAAAACGACAGCTCTTCAATTACCAAAGCTGCCAAAAAGGTGAAGGGCAACTTCTGTTTGGCCTTAACTGGTACGCCAATAGAAAACCGCTTGTTGGACCTTTGA", "aa": "MYEKLTNEILIVSFNVLRSDVKLITKQRFHYVVIDEAQGIKNDSSSITKAAKKVKGNFCLALTGTPIENRLLDL", "aa_hexdigest": "2f074a5cf1e1038ab1f889e593fc6949", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 69, "aa_cov": 0.9324324324324325, "hmm_cov": 1.0, "evalue": 4.209582706305435e-17, "score": 57.59275817871094, "start": 6, "stop": 74 } ], "seq_stats": { "molecular_weight": 8353.668099999999, "isoelectric_point": 9.206359291076659 }, "id": "CNOCLNGIAL_81", "locus": "GCA000143945_00041" }, { "type": "cds", "sequence": "contig_1", "start": 25249, "stop": 25884, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37", "PFAM:PF00176.29" ], "nt": "GTGCTCCCAAGCTTTTTAGGGAACAAAAAGCAGTTTACCGACCAGTTTGAAAAGGAAAAAACCGACCAAAGCTTCCATTTGTTAATGCAGCGCACTAGTCCCTTTATTTTGCGTCGTACCAAGTCCAAGGTCTTGAAGGAACTGCCTAACAAAATTACCACCGATATTTACGTAGAATTAAACCCAATGCACCAGAAGCTGTATGAGGAAGAACGTGACCGCGGTTTGGAGGAAATTAAGCAAATCCAAGACAAGAGTTCCTTTAACATCTTGACTTTAATCTTGAAGTTAAGGCACTTGTGTAGTTTACCCAAAAACAGTCAGGGTATTTTGGAAAACAGTGCCAAAAAGGAAGCTGCTTTAGAAATTATCCACGAAGCGATTGAAAACCAGCGCAAGATTATCCTCTTTACCCAGTTCATTGATGTAATTGACCACTTTAAAGACACCTTTAAAGAGCAGGGCATTGAGTACTTTATCTTTGATGGCCGTAAGTCTCCTAAAAGCCGTCACAGTATTATCGAGAAGTTCAACAATGCCAAAAACCCTTGTGTGTTGTTAGCGTCCTTGAAAGCAGGGGGTGTGGGAATTAACCTCACTGCCGCTGAAGTGGTAATCCACTTTGATGTGTGGTGA", "aa": "MLPSFLGNKKQFTDQFEKEKTDQSFHLLMQRTSPFILRRTKSKVLKELPNKITTDIYVELNPMHQKLYEEERDRGLEEIKQIQDKSSFNILTLILKLRHLCSLPKNSQGILENSAKKEAALEIIHEAIENQRKIILFTQFIDVIDHFKDTFKEQGIEYFIFDGRKSPKSRHSIIEKFNNAKNPCVLLASLKAGGVGINLTAAEVVIHFDVW", "aa_hexdigest": "fbae1f9d8d0b765c8039e2539f9645fd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 94, "aa_cov": 0.44075829383886256, "hmm_cov": 0.9468085106382979, "evalue": 1.1489094817268634e-18, "score": 62.93749237060547, "start": 119, "stop": 211 }, { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 109, "aa_cov": 0.4834123222748815, "hmm_cov": 1.0, "evalue": 3.9255488385553754e-14, "score": 47.84605407714844, "start": 3, "stop": 104 } ], "seq_stats": { "molecular_weight": 24498.081100000018, "isoelectric_point": 8.939589118957521 }, "id": "CNOCLNGIAL_82", "locus": "GCA000143945_00042" }, { "type": "cds", "sequence": "contig_1", "start": 25894, "stop": 26100, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAACCAAGCGACTGACCGGGCCCACCGCATAGGACAAAAGAAAACAGTCCAAGTTTACCGCATTATTGCTAAAAATACGATTGAAGAGCGGGTCTGTCAAGTGCAGGCCGAAAAACAGGAGTTAGTGAGCAAAACGCTAGTGGAAGACGTCAATTTTTTCGAATCCTTGACCAATGAGGAATTATTACGTCTTTTTGAATAA", "aa": "MENQATDRAHRIGQKKTVQVYRIIAKNTIEERVCQVQAEKQELVSKTLVEDVNFFESLTNEELLRLFE", "aa_hexdigest": "541de4aabdea3cbc664f5d836f4aae02", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8009.008499999999, "isoelectric_point": 5.4145620346069325 }, "id": "CNOCLNGIAL_83", "locus": "GCA000143945_00043" }, { "type": "cds", "sequence": "contig_1", "start": 26146, "stop": 27318, "strand": "+", "frame": 1, "gene": "dnaJ", "product": "Chaperone protein DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085", "RefSeq:WP_010874378.1", "SO:0001217", "UniParc:UPI000012958B", "UniRef:UniRef100_P78004", "UniRef:UniRef50_P78004", "UniRef:UniRef90_P78004" ], "nt": "ATGGCCGCTGGTAAACGTGATTATTATGAAGTGTTAGGCGTTTCCAGATCCGCCACCGCACAAGACATCAAACGAGCTTTCCGCAAGCTTGCTATGCAGTACCACCCCGACCGCCATAAGGGTGAGGGTGAGACTGTCCAAAAACAAAACGAAGAGAAGTTTAAAGAGGTCAATGAGGCGTACGAGGTCTTAAGTGACACCGAGAAGCGTGGTATGTACGACCGCTTTGGCCATGAAGGGTTAAATGCCTCTGGCTTCCATGAAACTGGGTTTAATCCCTTTGACATCTTTAACAGTGTCTTTGGTGAGGGCTTTTCGTTTGACATGGACGGGGGTTCACCGTTTGACTTTATCTTTAGTAGGGGCAAAAAGTCGCGGCAAAACCGGGTTGTTTTGCCCTATGATTTAGAGATTGCGGTGGGGGTAGACATTAGCTTCTTTGAGATGACTAATGGTTGTACTCGGACGATTGAATATACCAAACGGGTGACCTGTAGTGCCTGTGATGGTTTTGGGGCTGAAGGGGGAGAAACCGGGATGGTTAGCTGTAACAGTTGTGAAGGCAATGGTTTTATTTTGAAGAATCAACGCTCCATCTTTGGTACAGTCCAGTCCCAGATGTTGTGTCAGAGCTGTGGTGGTCAGGGTAAACAGGCCAAACACAAGTGTAAGACTTGTAAGGGAAGTAAGTACAAGAAGGTACCAACTACCAAGGAAATTCAGATTCCCGCTGGCATTTACCATGGTGATGCGTTAGTCGATGACCATGGTGGTAATGAATTCGGGGGTCACGTTGGCAAGTTAGTGGTCCATGTGGGGGTACTACCGAGTAAGATCTTTAAACGGGTTGACCATAATGTAGTAGCCAATGTTTTAGTCGATCCGATGATTGCTATCACCGGAGGGAAAATTGAACTCCCTACCTTAGAAGGCATTAAGGAATTCAACTTACGTGCTGGTACTAAGAGTGGCGAGCAAATAGTAATCCCTGGTGGTGGGATTAAATTTACGAAGAACTTCCGCAAGAAGGCCGGGGATCTCATTATTATTATCAGTTATGCCCGTCCAAGTGAATACAGTGCGCCAGAGTTGCGCAAGCTCAAGGAATTTATTAAACCTAATCAAGAGGTAAAACAATATTTAAACGCACTTAAGAATGAATACAAGTCCTAA", "aa": "MAAGKRDYYEVLGVSRSATAQDIKRAFRKLAMQYHPDRHKGEGETVQKQNEEKFKEVNEAYEVLSDTEKRGMYDRFGHEGLNASGFHETGFNPFDIFNSVFGEGFSFDMDGGSPFDFIFSRGKKSRQNRVVLPYDLEIAVGVDISFFEMTNGCTRTIEYTKRVTCSACDGFGAEGGETGMVSCNSCEGNGFILKNQRSIFGTVQSQMLCQSCGGQGKQAKHKCKTCKGSKYKKVPTTKEIQIPAGIYHGDALVDDHGGNEFGGHVGKLVVHVGVLPSKIFKRVDHNVVANVLVDPMIAITGGKIELPTLEGIKEFNLRAGTKSGEQIVIPGGGIKFTKNFRKKAGDLIIIISYARPSEYSAPELRKLKEFIKPNQEVKQYLNALKNEYKS", "aa_hexdigest": "c15a02691b10973fc889082929c5eaf5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012958B", "ncbi_nrp_id": "WP_010874378.1", "uniref100_id": "UniRef100_P78004", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012958B", "RefSeq:WP_010874378.1", "UniRef:UniRef100_P78004" ] }, "ips": { "uniref100_id": "UniRef100_P78004", "uniref90_id": "UniRef90_P78004" }, "psc": { "uniref90_id": "UniRef90_P78004", "gene": "dnaJ", "product": "Chaperone protein DnaJ", "uniref50_id": "UniRef50_P78004", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0031072", "GO:0051082", "GO:0008270", "GO:0051085", "GO:0006260", "GO:0042026", "GO:0009408" ] }, "pscc": { "uniref50_id": "UniRef50_P78004", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78004" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "CNOCLNGIAL_84", "locus": "GCA000143945_00044" }, { "type": "cds", "sequence": "contig_1", "start": 27479, "stop": 28231, "strand": "+", "frame": 2, "gene": "pip", "product": "prolyl aminopeptidase", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.4.11.5", "GO:0004177", "GO:0005737", "GO:0006508", "GO:0030163", "SO:0001217", "UniRef:UniRef50_P75092", "UniRef:UniRef90_P75092" ], "nt": "TTGTTAGACCAACGGGGTTGTGGTCAAAGTAAAGCGGTTAATCCCTTACTGCACAACACTACCCAAGACTTAGTTGGGGACTTGGAAGCGTTGCGCCAACACTTAAAACTTGAGCGTTGGACCCTATTTGGTGGTAGTTGGGGTTCTACTTTAGCCTTAGTGTATGCCATTACCCATCCCCAAGTAGTCGAACAAGTCTTTTTAAGAGCGCTCTTTTTGGGCCGTGAACAAGACTGGGCTGAAATGCTGTTGGGCTTGGGCAAGCTCTTCTACCCTTACGAACACCAAACGTTACTGAAAGCCATTCCCCAGGCTTGTCGCACTGACTTTACGAAATTCACTAACTACTTTTATGAAGTGTTGCAAGGTAACGATTCCGCTTTAAAAACGCAGTTAGCCAACGCTTGGGTTAAGTGGGAAAATACCTTACTATCACCGATTAGCTATGTCAAAGACGAGAAAGCAGAAGATGCGAACTTTACCTTTAAGTTAGCGCTCTTGGAATGTCACTACGCAAAGCACCACAGCTTTTTAAAACCGAACTTTATCCTGGAAAATGTTGCGGTATTAAAGGACAAACCGGTGCACTTAATCCACGGCCGTTTTGATCTAGTCTGTCCGTTAAGTCAAGCCTTGGAACTCAAACGTGCTTTACCAACGCTTAACTTGTATGTAACTAACAATGCCGGGCACAGTGGCAGTGATCCCAATAACCTCACTACCATTAAACACCTTTTAAAAACACAACTGTAA", "aa": "MLDQRGCGQSKAVNPLLHNTTQDLVGDLEALRQHLKLERWTLFGGSWGSTLALVYAITHPQVVEQVFLRALFLGREQDWAEMLLGLGKLFYPYEHQTLLKAIPQACRTDFTKFTNYFYEVLQGNDSALKTQLANAWVKWENTLLSPISYVKDEKAEDANFTFKLALLECHYAKHHSFLKPNFILENVAVLKDKPVHLIHGRFDLVCPLSQALELKRALPTLNLYVTNNAGHSGSDPNNLTTIKHLLKTQL", "aa_hexdigest": "c9361cf0dd788333a54a3bbd304fb246", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75092", "query_cov": 1.0, "subject_cov": 0.8090614886731392, "identity": 0.996, "score": 513.0, "evalue": 2.13e-183, "valid": true, "gene": "pip", "product": "prolyl aminopeptidase", "ec_ids": [ "3.4.11.5" ], "uniref50_id": "UniRef50_P75092", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0004177", "GO:0005737", "GO:0006508", "GO:0030163" ] }, "pscc": { "uniref50_id": "UniRef50_P75092", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75092" ], "product": "Putative proline iminopeptidase" }, "genes": [ "pip" ], "id": "CNOCLNGIAL_85", "locus": "GCA000143945_00045" }, { "type": "cds", "sequence": "contig_1", "start": 28231, "stop": 28473, "strand": "+", "frame": 1, "gene": null, "product": "Methionine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M6W2H4" ], "nt": "ATGAAACGGTGTTACATTACCACCCCGATTTACTACGCCTCGGGTAAGCCCCACATTGGCCATGCCTTTACGACCATCTTAGCGGATGTGATTAAGCGGTATAAACAGCAAAACGGTTATGAAGCTTACTTTCTAACTGGTACGGATGAACACGGCAACAAAATTGAAAGTAAGGCCAAAAGTTTAGGTCTAGATCCCCAAACCTTTGTGGATCAAAACGTGGCTTACTTTCAACAAATGTGA", "aa": "MKRCYITTPIYYASGKPHIGHAFTTILADVIKRYKQQNGYEAYFLTGTDEHGNKIESKAKSLGLDPQTFVDQNVAYFQQM", "aa_hexdigest": "e42754332f3ed166bcbd7561bddc9cc2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003620B786", "query_cov": 0.8375, "subject_cov": 0.9054054054054054, "identity": 0.5820000000000001, "score": 90.5, "evalue": 2.34e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A2M6W2H4" }, "pscc": { "uniref50_id": "UniRef50_A0A2M6W2H4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M6W2H4" ], "product": "Methionine--tRNA ligase" }, "genes": [], "id": "CNOCLNGIAL_86", "locus": "GCA000143945_00046" }, { "type": "cds", "sequence": "contig_1", "start": 28651, "stop": 29049, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTGTGAAATAGGTCACCAGTTAACCCTAGTCCAAGAACCATCTTTATTTATTGCCTTTAAAGATAGTAAGGACTGGATTGGGGAAATGATTGCGACAAACAAGCTCAACATTACCCCTGAATCCAGAGCCGCTGAGTTAAAGAACAACTTCTTGGATGGGGGTTTAAATGACTTAGCTTTAACACGGCAAAACGTGACTTGGGGAATTCCCGTACCCTTTGACAACAAGCAAACGATTTACGTGTGGTTTGATGCCTTGTTTAACTACATTACTAACTTAGGGTTTGCCCATAACGACCCTAAATTTAACAAATGGTGGAACAATAATGATGAGGAACACGAAGTCATTCATTTAATTTCACGCGAAATTACCCGTTTCCACTGCATTTACTGA", "aa": "MLCEIGHQLTLVQEPSLFIAFKDSKDWIGEMIATNKLNITPESRAAELKNNFLDGGLNDLALTRQNVTWGIPVPFDNKQTIYVWFDALFNYITNLGFAHNDPKFNKWWNNNDEEHEVIHLISREITRFHCIY", "aa_hexdigest": "6c8fca86a153831eb582f9744a053720", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15439.314699999992, "isoelectric_point": 5.234893989562988 }, "id": "CNOCLNGIAL_87", "locus": "GCA000143945_00047" }, { "type": "cds", "sequence": "contig_1", "start": 29068, "stop": 29442, "strand": "+", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310053", "UniRef:UniRef90_UPI0013310053" ], "nt": "TTGGGTTTTAAACTACCGACCCAGTTTCTCTCCCATGGTTGGATTGTCGATGGTAATGGGCACAAGATGTCCAAGTCGTTGGGCAATGTCATTTCCCCCGAAGAACTATTGGCCCAGTTTGGTGTTGATGGTACGCGTTATTGCTTACTTAAAGAAATGCGCTTGGACAAGGACAACCGCTGTAGTATGGCGATCTTTAAGGACATTTACAACGCTGATTTGGCCAACAGTTTTGGTAACCACGCATCACGTACCTTTGGCATGATCAAAAAGTACTTGGGCGGCCAGTTAGACTTTATCGAAGTCCAAGACCCTCAAGTAAAGCAGCTAATGGACCAAGCGGACCAAGCGATGGTTCAGTTTGACACCGCTTGA", "aa": "MGFKLPTQFLSHGWIVDGNGHKMSKSLGNVISPEELLAQFGVDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHASRTFGMIKKYLGGQLDFIEVQDPQVKQLMDQADQAMVQFDTA", "aa_hexdigest": "310817fc2d67953a17079e2085406933", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 28984, "stop": 29745, "observations": { "mutation": [ 29047, 29440 ], "stop": [ 29047, 29440 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013310053", "query_cov": 1.0, "subject_cov": 0.47509578544061304, "identity": 0.992, "score": 252.0, "evalue": 5.65e-83, "gene_start": 1, "gene_end": 124, "reference_start": 29, "reference_end": 152, "reference_sequence": "DEEHEVIHLISREITRFLCIYWPIFLHQLGFKLPTQFLSHGWIVDGNGHKMSKSLGNVISPEELLAQFGVDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHASRTFGMIKKYLGGQLDFIEVQDPQVKQLMDQADQAMVQFDTAWNNFQFYKGINGLLQLVFQASKLIDQLKPWELVKQTDYTLLKQLLFACVRCTQVCFVLLAPILVHTSTQIFDLFNFSAQARSKTHLADPQQLQKISLAPVIQLLFKRLD" }, "paralog": false, "identity": 0.9881422924901185, "score": 502.0, "evalue": 4.73e-184, "description": "Internal stop codon at 29047, 29440. Nonsense mutation around 29047, 29440.", "psc": { "uniref90_id": "UniRef90_UPI0013310053", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0013310053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013310053", "UniRef:UniRef50_UPI0013310053" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013310053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310053" ], "product": "class I tRNA ligase family protein" } }, "id": "CNOCLNGIAL_88", "locus": "GCA000143945_00048" }, { "type": "cds", "sequence": "contig_1", "start": 29599, "stop": 29769, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGCTTTGTCTTGTTAGCGCCGATCTTAGTTCATACCAGTACCCAGATATTTGACCTGTTTAACTTCAGTGCCCAAGCACGTAGTAAGACCCACCTAGCTGATCCACAACAACTGCAAAAAATCAGCTTAGCACCTGTAATTCAACTTTTATTTAAAAGATTGGATTAA", "aa": "MCFVLLAPILVHTSTQIFDLFNFSAQARSKTHLADPQQLQKISLAPVIQLLFKRLD", "aa_hexdigest": "8bfaafa6e1e3a50befcb53a9eba3718f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6367.485500000001, "isoelectric_point": 9.186051750183104 }, "id": "CNOCLNGIAL_89", "locus": "GCA000143945_00049" }, { "type": "cds", "sequence": "contig_1", "start": 29790, "stop": 29888, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTATGCAAGTAGAATACCTCGATCTCATCTCCCAAGCGAAGGAGATTGCTGAGACCCAATTTAAGGCGGAACCGTTTTCCTTTGATGCTATTTGA", "aa": "MTMQVEYLDLISQAKEIAETQFKAEPFSFDAI", "aa_hexdigest": "6974cd282a195b65a38f65d1f70d5bb3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3695.1731, "isoelectric_point": 4.07884578704834 }, "id": "CNOCLNGIAL_90", "locus": "GCA000143945_00050" }, { "type": "cds", "sequence": "contig_1", "start": 29898, "stop": 30230, "strand": "+", "frame": 3, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "COG:COG3343", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355", "KEGG:K03048", "SO:0001217", "UniRef:UniRef50_P47268", "UniRef:UniRef90_P75090" ], "nt": "GTGAAGCACTTTAAGATCTCCAAACAAGACGAACCTAACCTAATTTCGCGGTTTTACCAAGACTTTTTGGAAGACCCTAACTTTGTTTATTTAGGGGAACGCAACTGGAAGTTAAGGGACTTCATGAAGTTTGATGAATGGAACAAGATTTCCCAAGCAATGTTTGTGACCAAGGAAATCTTTGAAGAGGGTTATGAAGACCTGTCCAACAAGAAGAAGGAAAACGAGGAAGAAGTGAATGACTTCATTATGGGCAATGATGGTGATGACAACAGTACCGGTGATGAAATTGTGCAAGGACTCATTAACGATTTTCGCGACGATAACCAATAA", "aa": "MKHFKISKQDEPNLISRFYQDFLEDPNFVYLGERNWKLRDFMKFDEWNKISQAMFVTKEIFEEGYEDLSNKKKENEEEVNDFIMGNDGDDNSTGDEIVQGLINDFRDDNQ", "aa_hexdigest": "42aaa3b7158ae8156e8956ee3696c374", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 29790, "stop": 30230, "observations": { "mutation": [ 29886 ], "stop": [ 29886 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75090", "query_cov": 1.0, "subject_cov": 0.7534246575342466, "identity": 0.982, "score": 222.0, "evalue": 5.34e-73, "gene_start": 1, "gene_end": 110, "reference_start": 37, "reference_end": 146, "reference_sequence": "MTMQVEYLDLISQAKEIAETQFKAEPFSFDAIWKEVVKHFKISKQDEPNLISRFYQDFLEDPNFVYLGERNWKLRDFMKFDEWNKISQAMFVTKEIFEEGYEDLSNKKKENEEEVNDFIMGNDGDDNSTGDEIVQGLINDFRDDKQ" }, "paralog": false, "identity": 0.9863013698630136, "score": 294.0, "evalue": 7.07e-104, "description": "Internal stop codon at 29886. Nonsense mutation around 29886.", "psc": { "uniref90_id": "UniRef90_P75090", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47268", "cog_id": "COG3343", "cog_category": "K", "kegg_orthology_id": "K03048", "go_ids": [ "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75090", "EC:2.7.7.6", "UniRef:UniRef50_P47268", "COG:COG3343", "COG:K", "KEGG:K03048", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47268", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47268" ], "product": "Probable DNA-directed RNA polymerase subunit delta" } }, "id": "CNOCLNGIAL_91", "locus": "GCA000143945_00051" }, { "type": "cds", "sequence": "contig_1", "start": 30230, "stop": 30781, "strand": "+", "frame": 2, "gene": null, "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "nt": "ATGCTAGTAAACATCAAACAAATGTTGCAACACGCTAAGCAACACCACTATGCTGTACCCCACATTAATATCAATAACTATGAATGGGCTAAGGCAGTATTAACAGCGGCTCAGCAGGCCAAGAGTCCGATTATTGTCTCCACTTCTGAGGGCGCTTTAAAGTATATGTCCGGACACCAAGTGGTAGTACCGATGGTAAAGGGCTTAGTCGATGCTTTAAAGATTACTGTACCCGTAGCACTCCACTTAGACCATGGTAGTTATGAAGGTTGTAAGGCAGCGTTGCAAGCTGGGTTTAGTTCCATTATGTTTGATGGCTCGCATTTACCCTTTCAAGAAAACTTCACTAAATCCAAGGAGTTAATTGAACTAGCTAAACAAACCAATGCTTCTGTGGAACTAGAAGTGGGTACCTTAGGTGGGGAAGAAGATGGTATTGTGGGTCAAGGTGAATTGGCCAACATTGAAGAGTGCAAGCAAATTGCGACCTTAAAACCGGATGCCTTAGCTGCTGGGATTGGTAACATCCACGGGCTTTACCCCGACAACTGA", "aa": "MLVNIKQMLQHAKQHHYAVPHININNYEWAKAVLTAAQQAKSPIIVSTSEGALKYMSGHQVVVPMVKGLVDALKITVPVALHLDHGSYEGCKAALQAGFSSIMFDGSHLPFQENFTKSKELIELAKQTNASVELEVGTLGGEEDGIVGQGELANIEECKQIATLKPDALAAGIGNIHGLYPDN", "aa_hexdigest": "d177b04684ec03c182927d8ac06c541e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FEF401E", "query_cov": 0.9016393442622951, "subject_cov": 0.9375, "identity": 0.636, "score": 212.0, "evalue": 1.69e-67, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A3B0P7V3", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "product": "Fructose-bisphosphate aldolase" }, "genes": [], "id": "CNOCLNGIAL_92", "locus": "GCA000143945_00052" }, { "type": "cds", "sequence": "contig_1", "start": 30839, "stop": 31096, "strand": "+", "frame": 2, "gene": null, "product": "fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "nt": "GTGCTCCATGGTGGTTCGGGAATTCCGGAAGCGGATGTCAAAAAAGCAATTGGCTTAGGCATTAGCAAGCTCAACATTAACACTGAATGTCAACTAGCGTTTGCTAAAGCGATTCGGGAATATGTGGAAGCCAAGAAGGACTTAGATACCCACAATAAGGGCTATGACCCACGGAAATTGTTAAAGTCACCTACCCAAGCAATTGTGGACTGCTGCTTGGAAAAGATGCAGCTCTGTGGTTCAACCAATAAGGCTTAG", "aa": "MLHGGSGIPEADVKKAIGLGISKLNINTECQLAFAKAIREYVEAKKDLDTHNKGYDPRKLLKSPTQAIVDCCLEKMQLCGSTNKA", "aa_hexdigest": "75143f24b9163c163db3272e7b8045dc", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A097ST37", "query_cov": 1.0, "subject_cov": 0.8585858585858586, "identity": 0.647, "score": 119.0, "evalue": 2.17e-33, "valid": false, "product": "fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A097ST37", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A097ST37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "product": "fructose-bisphosphate aldolase" }, "genes": [], "id": "CNOCLNGIAL_93", "locus": "GCA000143945_00053" }, { "type": "cds", "sequence": "contig_1", "start": 31097, "stop": 32077, "strand": "+", "frame": 2, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P37518", "UniRef:UniRef90_P75088" ], "nt": "ATGTTAAGTGCGGGAATTGTTGGTTTACCCAACGTTGGTAAATCAACCTTGTTTAGTGCTATTACCAACCTGCAAGTGGAAATAGCTAACTATCCCTTTGCCACGATTGAACCGAATGCCGGGATAGTTAACGTAATCGATGAGCGCTTAGACAAACTAGCGAGTTTAATTAAACCCGATAAGGTTACCCACACTACCTTTCGCTTTGTTGACATTGCTGGTTTAGTTAAGGGTGCCAGTAAGGGAGAAGGACTGGGCAACCAGTTCTTGGCCAACATCAGAGAAGTTGACCTTATTTGTCACGTAGTGCGTTGCTATGAGGACAAGAAGATTGTCCACGTTAATAACCAGGTCGACCCCGTATTTGACTTTGAAATTATTGTCAATGAGTTAATCCAGGCCGACATTGAGGTGGTGAATACCCGGATTGGCAAGATTAAGCGCAAGGCCGAATCTGGTGATAAGCAGTCCAAGGAAGAATATCAACTGCTAGCACCCGTTTTACAAGGTTTGCAACAAAACCAGATGGTGTTGCATCTAGTGAATGAAGTAGATTTAAAGAAACTCAAGTCACTTAATTTACTAACCGCCAAACCGATCCTAGTGGTGGCCAATGTGTCAGAAGCGGATTTGTCCAACCTTGACCATAATCCCCACCTCACTCAGCTCAACCAGTTCTTAAAACAACATAACTTACCCCATGCCATTCCTGTTTGTGCATTATTAGAAAATGAGTTGAGTAGCTTGGATGCCAATGGTCGTCAAGACTGGCTCAAGGAGTTGGGTTTAAGTGATTACCAAGGTTTAAACCAACTAATTAAAACGGCTTATGATGCGATTGGTCTGTGGTCATTCTTTACCTTTGGTAAGCAAGAAGTGCGCGCTTGGGCTTTTAAAAAGGGTTGGTTAGCACCGCAGTGTGCGGGTGAAATTCACACTGACTTTGAACGTGGTTTTATTAAAGTAGAAGTAATTAGTTGA", "aa": "MLSAGIVGLPNVGKSTLFSAITNLQVEIANYPFATIEPNAGIVNVIDERLDKLASLIKPDKVTHTTFRFVDIAGLVKGASKGEGLGNQFLANIREVDLICHVVRCYEDKKIVHVNNQVDPVFDFEIIVNELIQADIEVVNTRIGKIKRKAESGDKQSKEEYQLLAPVLQGLQQNQMVLHLVNEVDLKKLKSLNLLTAKPILVVANVSEADLSNLDHNPHLTQLNQFLKQHNLPHAIPVCALLENELSSLDANGRQDWLKELGLSDYQGLNQLIKTAYDAIGLWSFFTFGKQEVRAWAFKKGWLAPQCAGEIHTDFERGFIKVEVIS", "aa_hexdigest": "3c04210cf453a8bcfb7d85eba911b15b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75088", "query_cov": 1.0, "subject_cov": 0.8907103825136612, "identity": 1.0, "score": 637.0, "evalue": 6.5e-230, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P37518", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P37518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P37518" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "CNOCLNGIAL_94", "locus": "GCA000143945_00054" }, { "type": "cds", "sequence": "contig_1", "start": 32322, "stop": 32411, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAACCAACTAAGAACCTCACCGTTAACCTAATTAACATTGGTTTTGATGCCTATTCCGACAGTCAAAAAGCGCTGTTAGCTTGA", "aa": "MLKPTKNLTVNLINIGFDAYSDSQKALLA", "aa_hexdigest": "969b4bf44870ad7e8a75185f124772ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3179.6838, "isoelectric_point": 8.18930606842041 }, "id": "CNOCLNGIAL_95", "locus": "GCA000143945_00055" }, { "type": "cds", "sequence": "contig_1", "start": 32601, "stop": 33011, "strand": "-", "frame": 1, "gene": "rimL", "product": "putative protein MPN_027", "db_xrefs": [ "COG:COG1670", "COG:JO", "GO:0016747", "SO:0001217", "UniRef:UniRef50_P75087", "UniRef:UniRef90_P75087" ], "nt": "ATGGATAAAAACACTATTCTTGAGTTTTGCTTTTTTGGTGATCGTTACCAACTAATGGCACTGAAAAACCCCAATAAGTTGGTCAAAAATAAACAGGTCAAAGCTGCTTTTAATGTTTTCATGAAGCACCGGACCACCTTAAGTGCTACTAACTATATGGCGCAAATTGTGGAAAACTTTGAGCAGTTCTTGGAATACATCCACGACAAACTGCAACCCGGTAAACTGTTAGCGCTCTTATTTAAAAACAAGCAGTTTTTGGGTTGGTTTTGTGTCGGGCAATGGGGCAACATGCCGGTATTATCGTTTTGGATTGATCCCAGTCAACAACAAAGTGGTGTCGCTTTAGAAACAATTAGTTTCTTTCTTAAGTACTACCAGCGTACGTTGCAACCGCGGGAATTAGTGTGA", "aa": "MDKNTILEFCFFGDRYQLMALKNPNKLVKNKQVKAAFNVFMKHRTTLSATNYMAQIVENFEQFLEYIHDKLQPGKLLALLFKNKQFLGWFCVGQWGNMPVLSFWIDPSQQQSGVALETISFFLKYYQRTLQPRELV", "aa_hexdigest": "b1a406132be46032d970c6555964dee1", "start_type": "ATG", "rbs_motif": null, "genes": [ "rimL" ], "truncated": "5-prime", "pseudogene": { "start": 32298, "stop": 33011, "observations": { "mutation": [ 32324, 32426, 32438, 32603 ], "stop": [ 32324, 32426, 32438, 32603 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75087", "query_cov": 1.0, "subject_cov": 0.49635036496350365, "identity": 1.0, "score": 283.0, "evalue": 9.72e-95, "gene_start": 1, "gene_end": 136, "reference_start": 1, "reference_end": 136, "reference_sequence": "MDKNTILEFCFFGDRYQLMALKNPNKLVKNKQVKAAFNVFMKHRTTLSATNYMAQIVENFEQFLEYIHDKLQPGKLLALLFKNKQFLGWFCVGQWGNMPVLSFWIDPSQQQSGVALETISFFLKYYQRTLQPRELVWSFYESNQRSRHLANKLGFPSLFYFKKAEPNAPKVYFGTKDFKGESVIETKPYLGWGSGWKVNQMLKPTKNLTVNLINIGFDAYADSQKALLAWFKTHKLTKGQVEVFFNYELCCLCISYNHKQSWVVYSFQQLLAKR" }, "paralog": false, "identity": 0.9789029535864979, "score": 469.0, "evalue": 1.07e-170, "description": "Internal stop codon at 32324, 32426, 32438, 32603. Nonsense mutation around 32324, 32426, 32438, 32603.", "psc": { "uniref90_id": "UniRef90_P75087", "gene": "RimL", "product": "Uncharacterized protein MPN_027", "uniref50_id": "UniRef50_P75087", "cog_id": "COG1670", "cog_category": "JO", "go_ids": [ "GO:0016747" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75087", "UniRef:UniRef50_P75087", "COG:COG1670", "COG:JO", "GO:0016747" ] }, "pscc": { "uniref50_id": "UniRef50_P75087", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75087" ], "product": "Uncharacterized protein MPN_027" } }, "id": "CNOCLNGIAL_96", "locus": "GCA000143945_00056" }, { "type": "cds", "sequence": "contig_1", "start": 33044, "stop": 33265, "strand": "+", "frame": 2, "gene": null, "product": "glycosyltransferase family 2 protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED93A4E" ], "nt": "GTGCAATTCAAGTATTTATTCACCGTGATTATTCCCACTTACAATTGTGGTCAATACATCCCAAAAGCATTAGATTCCTTGCTTTTACAAGGTGAATATTTTACTAAGACCCAAGTTTTAATCGTCAATGATGGTTCGACTGATAATACCAAGCAGATCGTAGAACCCTATACCCAACAATACAGTAACATTGAGTACTTGGAAAAACCCAATGGCAATTGA", "aa": "MQFKYLFTVIIPTYNCGQYIPKALDSLLLQGEYFTKTQVLIVNDGSTDNTKQIVEPYTQQYSNIEYLEKPNGN", "aa_hexdigest": "e4c2ed2ab68fc1a238982e4aaf22c83d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED93A4E", "query_cov": 1.0, "subject_cov": 0.8588235294117647, "identity": 0.767, "score": 113.0, "evalue": 1.71e-31, "valid": false, "product": "glycosyltransferase family 2 protein", "uniref50_id": "UniRef50_UPI001ED93A4E" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED93A4E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED93A4E" ], "product": "glycosyltransferase family 2 protein" }, "genes": [], "id": "CNOCLNGIAL_97", "locus": "GCA000143945_00057" }, { "type": "cds", "sequence": "contig_1", "start": 33272, "stop": 33697, "strand": "+", "frame": 2, "gene": "wcaA", "product": "putative glycosyltransferase MG025-like protein", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.-.-", "GO:0016757", "SO:0001217", "UniRef:UniRef50_P75086", "UniRef:UniRef90_P75086" ], "nt": "GTGGTCAACTTTGTCAAACAAAACCAGTTAGCTAAGGGTCAGTACATTACTGTATTAGACAGCGATGATTATTTTCTAGCTAACGCTTTTCAACGCGTAGCTGCTCACTTTGGCCATGACATGATTGTGAGCGCCTTTTACTGTTACATTAGCCCTAAAAGAAGGCGTTTTCTCAAACCATACTTTGGTAAAACGGGGGTAATTGAACAAAAGACTAAACTACGCACACCCCATTCCCAACCCTTGGCTAAGTTCTACCGCCACGAGATCTTTAACTTGCTAGATCCTTTAAAAGAAAAGCTGTTTTACCAGGACTGTCTGTTGTACCATAATGCCATCAATAAGGTGCAAAGTGTCTTTTACATTTGTGAACCCTTAGCAGTGTGGTATGCCACTAGACCAGGTAATTCCACCACTATGCCCTGA", "aa": "MVNFVKQNQLAKGQYITVLDSDDYFLANAFQRVAAHFGHDMIVSAFYCYISPKRRRFLKPYFGKTGVIEQKTKLRTPHSQPLAKFYRHEIFNLLDPLKEKLFYQDCLLYHNAINKVQSVFYICEPLAVWYATRPGNSTTMP", "aa_hexdigest": "2c9cbb74b0c22c6b4fa1505e9225ee48", "start_type": "GTG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "both", "pseudogene": { "start": 33044, "stop": 33943, "observations": { "mutation": [ 33263, 33695, 33860 ], "stop": [ 33263, 33695, 33860 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75086", "query_cov": 1.0, "subject_cov": 0.47157190635451507, "identity": 0.986, "score": 292.0, "evalue": 6.12e-98, "gene_start": 1, "gene_end": 141, "reference_start": 77, "reference_end": 217, "reference_sequence": "MQFKYLFTVIIPTYNCGQYIPKALDSLLLQGEYFTKTQVLIVNDGSTDNTKQIVEPYTQQYSNIEYLEKPNGNWGSVVNFVKQNQLAKGQYITVLDSDDYFLANAFQRVAAHFGHDMIVSAFYCYISPKRRRFLKPYFGKTGVIEQKTKLRTPHSQPLAKFYRHEIFHLLDPLKEKLFYQDCLLYHNAINKVQSVFYICEPLAVWYATRPGNSTTMPWNNADKFQAWCDLLKQMNLYGAGIVIYIYTMLPGFLKELKRQQLVLDLAKKPAYTWLPQPLAFLFGGLMALRTRKYIRYPKN" }, "paralog": false, "identity": 0.9832775919732442, "score": 602.0, "evalue": 9.1e-223, "description": "Internal stop codon at 33263, 33695, 33860. Nonsense mutation around 33263, 33695, 33860.", "psc": { "uniref90_id": "UniRef90_P75086", "gene": "WcaA", "product": "Uncharacterized glycosyltransferase MG025 homolog", "ec_ids": [ "2.4.-.-" ], "uniref50_id": "UniRef50_P75086", "cog_id": "COG0463", "cog_category": "M", "go_ids": [ "GO:0016757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75086", "EC:2.4.-.-", "UniRef:UniRef50_P75086", "COG:COG0463", "COG:M", "GO:0016757" ] }, "pscc": { "uniref50_id": "UniRef50_P75086", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75086" ], "product": "Uncharacterized glycosyltransferase MG025 homolog" } }, "id": "CNOCLNGIAL_98", "locus": "GCA000143945_00058" }, { "type": "cds", "sequence": "contig_1", "start": 33743, "stop": 33862, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTATACGGCGCGGGTATAGTGATTTACATTTACACTATGTTACCTGGTTTTTTAAAAGAATTAAAACGGCAACAACTAGTGTTAGATTTAGCTAAAAAACCCGCTTATACCTGA", "aa": "MNLYGAGIVIYIYTMLPGFLKELKRQQLVLDLAKKPAYT", "aa_hexdigest": "3898c01e7da5abe6be54c273b7f2bc05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4474.375000000002, "isoelectric_point": 9.522770118713382 }, "id": "CNOCLNGIAL_99", "locus": "GCA000143945_00059" }, { "type": "cds", "sequence": "contig_1", "start": 33964, "stop": 34536, "strand": "+", "frame": 1, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043", "KEGG:K02356", "RefSeq:WP_010874386.1", "SO:0001217", "UniParc:UPI0000129D0D", "UniRef:UniRef100_P75085", "UniRef:UniRef50_P75085", "UniRef:UniRef90_P75085" ], "nt": "ATGGCAGACATGATTGAAGCGAAAAGTTTACGCAGTGGACAAACGATTTTTGGTCCCAATAAAGAGATTCTGTTGGTGTTAGAAAACACCTTTAATAAGACCGCAATGCGCCAGGGAATTGTTAAAACCAAGGTCAAAAACTTACGCACTGGGGCCATTGTTTGGATTGAGTTTACCGGAGACAAGTTAGAACAAGTCATTATTGACAAGAAGAAAATGACCTTCTTGTACAAGGATGGTGCTAACTATGTTTTTATGGACCAACAAGACTACAGTCAAATTGAGATCCCCGAAAAGCAGTTAGAGTGGGAAAAGAACTTTATCACTGAAGACAGTGAAGTTACTATTATTAGCTACCAAAGTGAAATTTTGGGCGTTAACCTACCCGAATTAGTGCCGATTGAAGTGGAATTTGCCGAAGAAGCAGTGCAAGGTAATACCGCTAACATGGCGCGTAAACGGGCTCGGTTAGTTTCGGGTTACGAATTAGATGTGCCCCAGTTCATTCGTACTGGTGATAAGATTGTGATTTCCACCATTGACGGGAGCTATCGTGAACGGTACAACAAGTAA", "aa": "MADMIEAKSLRSGQTIFGPNKEILLVLENTFNKTAMRQGIVKTKVKNLRTGAIVWIEFTGDKLEQVIIDKKKMTFLYKDGANYVFMDQQDYSQIEIPEKQLEWEKNFITEDSEVTIISYQSEILGVNLPELVPIEVEFAEEAVQGNTANMARKRARLVSGYELDVPQFIRTGDKIVISTIDGSYRERYNK", "aa_hexdigest": "ed93e08bd627e0e94ccd9d96fdcbd1c0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129D0D", "ncbi_nrp_id": "WP_010874386.1", "uniref100_id": "UniRef100_P75085", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129D0D", "RefSeq:WP_010874386.1", "UniRef:UniRef100_P75085" ] }, "ips": { "uniref100_id": "UniRef100_P75085", "uniref90_id": "UniRef90_P75085" }, "psc": { "uniref90_id": "UniRef90_P75085", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_P75085", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ] }, "pscc": { "uniref50_id": "UniRef50_P75085", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75085" ], "product": "Elongation factor P" }, "genes": [ "efp" ], "id": "CNOCLNGIAL_100", "locus": "GCA000143945_00060" }, { "type": "cds", "sequence": "contig_1", "start": 34517, "stop": 34960, "strand": "+", "frame": 2, "gene": "nusB", "product": "putative MG027-like protein", "db_xrefs": [ "COG:COG0781", "COG:K", "RefSeq:WP_014574845.1", "SO:0001217", "UniParc:UPI0001DD6462", "UniRef:UniRef100_A0AAN4XFP4", "UniRef:UniRef50_P47273", "UniRef:UniRef90_P75084" ], "nt": "GTGAACGGTACAACAAGTAAATTAACTAGAACCCAAAGACGGATTGCAATTGTGGAGTTTATCTTCGCCACGCTGTTCTTTTTACCGAAAACTGCTGACCAAATCCAAGCGGCCTTTTTGGACTATGATGTGCCAGAACGTCCGCTCAACGATTGGCAAAAAGAGATTGTCAAAGTCTTTAGTGAACGTTGTGTGGAATTCATTGAACTAATTGAAAACCAACAACAACGCAACCAAGCAGAAGTACAAAGCAAGTACAACAAGGTTTCCGGTAAAAAAGTCGACTTATTGACTAAGGCCGTGATTCTCTGTGCTTTGAGCGAACAACACGCGCAAGCGACTGATAAACCATTATTAATTAGTGAAGCCTTACTGATTATGGACCACTATTCCCAGGTACCCGAAAAGAAGCAAACCCATGCTTTATTGGATAAACTCTTGTAA", "aa": "MNGTTSKLTRTQRRIAIVEFIFATLFFLPKTADQIQAAFLDYDVPERPLNDWQKEIVKVFSERCVEFIELIENQQQRNQAEVQSKYNKVSGKKVDLLTKAVILCALSEQHAQATDKPLLISEALLIMDHYSQVPEKKQTHALLDKLL", "aa_hexdigest": "e029943092bf8c0c09f57c14baf6f38b", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6462", "ncbi_nrp_id": "WP_014574845.1", "uniref100_id": "UniRef100_A0AAN4XFP4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6462", "RefSeq:WP_014574845.1", "UniRef:UniRef100_A0AAN4XFP4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAN4XFP4", "uniref90_id": "UniRef90_P75084" }, "psc": { "uniref90_id": "UniRef90_P75084", "gene": "NusB", "product": "Uncharacterized protein MG027 homolog", "uniref50_id": "UniRef50_P47273", "cog_id": "COG0781", "cog_category": "K" }, "pscc": { "uniref50_id": "UniRef50_P47273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47273" ], "product": "Uncharacterized protein MG027" }, "genes": [ "nusB" ], "id": "CNOCLNGIAL_101", "locus": "GCA000143945_00061" }, { "type": "cds", "sequence": "contig_1", "start": 34990, "stop": 35199, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGTAGCAAACCTAGCCTTAGTGTTGGGTTTTATGTTGAACATTGTGGTGGCGCGGTATACCTTAACTGGTGCTACGCCCCAAGCACGTTTTTTATTCCTCACACCCTTTTTAGGCATAGTAGCAGCGAGTATCTTCTACTTCTTTGATGTGAAGTGGTTCTTAGCGGATTATCCCTACAAGAAGTTCCATTTTCAAAAGAAGTGA", "aa": "MFVANLALVLGFMLNIVVARYTLTGATPQARFLFLTPFLGIVAASIFYFFDVKWFLADYPYKKFHFQKK", "aa_hexdigest": "7c4ba972575b761923c09e97ff050c0d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8018.525199999996, "isoelectric_point": 9.90029697418213 }, "id": "CNOCLNGIAL_102", "locus": "GCA000143945_00062" }, { "type": "cds", "sequence": "contig_1", "start": 35254, "stop": 35349, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTGTTAGCACTCCTCGTTAACCAGATGACCAACCAAATCCTTAGTGAAAAGTATACGGGTTTACTGGACAACGCTTACCCACTACTTTGA", "aa": "MILLALLVNQMTNQILSEKYTGLLDNAYPLL", "aa_hexdigest": "64b48ec3a0aa837a6a28ddd54462a5f4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3507.1656000000016, "isoelectric_point": 4.369065666198731 }, "id": "CNOCLNGIAL_103", "locus": "GCA000143945_00063" }, { "type": "cds", "sequence": "contig_1", "start": 35356, "stop": 35571, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTGTTAGTATCTTTTTGAGCTTAATTAGTATTGGTTTGAGCAAAACAGCCCACTTTAAAATTGATGTGGAAATGCTCAAAGCGAAAAAAGGCGAACCCACCGCCGCTGATAAAACCGACAGCCGGCCGGTAGTTGTTGATTTAGACCAAACTAAATCAAAAAAAGATGGGGATAACCCACCCCAAGCTTCGGGTGATATGACTAGTCTTTAG", "aa": "MGVSIFLSLISIGLSKTAHFKIDVEMLKAKKGEPTAADKTDSRPVVVDLDQTKSKKDGDNPPQASGDMTSL", "aa_hexdigest": "b66b2a8944333d45e3b66390bb9894b4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7560.590500000006, "isoelectric_point": 6.538212013244629 }, "id": "CNOCLNGIAL_104", "locus": "GCA000143945_00064" }, { "type": "cds", "sequence": "contig_1", "start": 35795, "stop": 36121, "strand": "-", "frame": 2, "gene": "yajL", "product": "putative MG029-like protein", "db_xrefs": [ "COG:COG0693", "COG:V", "GO:0005737", "RefSeq:WP_010874389.1", "SO:0001217", "UniParc:UPI0000139135", "UniRef:UniRef100_P75082", "UniRef:UniRef50_P75082", "UniRef:UniRef90_P75082" ], "nt": "ATGAAAAAACTCTTAGTCATTGTTTACCCTGACATGAATGATGTGGAATACACCAACACAATGGTGGTATTTGGTTTTGTTAAAGAATTACAAACCGTGATTTATCACCCTAACTTAAGTACAGTTAAGGGCAGTAATGGGGTAACTTTAGTAAACCAGATTACTAGCAAGGTTAATCTGGAGGAATTTGATGGTGTCTTTATCCCTGGCGGTATGGGAGCTACCAAGGTATTAGACCATGACCAACAGTTACTCGACACTATCCGTTACTTTAAGGATCATGACAAGTATGTCTTTGCCATTTGTGATACCCCCAACGTGTTGTAA", "aa": "MKKLLVIVYPDMNDVEYTNTMVVFGFVKELQTVIYHPNLSTVKGSNGVTLVNQITSKVNLEEFDGVFIPGGMGATKVLDHDQQLLDTIRYFKDHDKYVFAICDTPNVL", "aa_hexdigest": "df17031a769821fbbb19ec1969c9a74d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139135", "ncbi_nrp_id": "WP_010874389.1", "uniref100_id": "UniRef100_P75082", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139135", "RefSeq:WP_010874389.1", "UniRef:UniRef100_P75082" ] }, "ips": { "uniref100_id": "UniRef100_P75082", "uniref90_id": "UniRef90_P75082" }, "psc": { "uniref90_id": "UniRef90_P75082", "gene": "YajL", "product": "Uncharacterized protein MG029 homolog", "uniref50_id": "UniRef50_P75082", "cog_id": "COG0693", "cog_category": "V", "go_ids": [ "GO:0005737" ] }, "pscc": { "uniref50_id": "UniRef50_P75082", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75082" ], "product": "Uncharacterized protein MG029 homolog" }, "genes": [ "yajL" ], "id": "CNOCLNGIAL_105", "locus": "GCA000143945_00065" }, { "type": "cds", "sequence": "contig_1", "start": 36125, "stop": 36745, "strand": "-", "frame": 2, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206", "RefSeq:WP_010874390.1", "SO:0001217", "UniParc:UPI0000137D2C", "UniRef:UniRef100_P75081", "UniRef:UniRef50_P47276", "UniRef:UniRef90_P75081" ], "nt": "GTGATCAAAAAAGTGCACCACGCTTTGATCGAAAACGAATTGACAAAGCTACGTGATAAATCTTCTTCCTCCTCGCAATTTCGGATGGCGTTAAGCCAAATCACTTCACTACTCTTTTTTGAAGTAACTAAGGAATCACCCTTAGAAGAAATTGTTGTAAAAACCCCGTTTACCAAAACGAAGGGTTATAAGCTCAAAAACGAAATTGTTCTAGTCCCCATAATGCGCGCTGGCCTGGGTATGGTTGACCCAATTGTGCGCTATTCGGAAAAGATTCGTGTGGGTCACTTAGGGATCTACCGTGAACATGAGGGCACTAATGTAATTTCTTACTATAAGAAAATGCCCGAAAACATTGGCAACTCCCACGTGATTATCTTAGACCCAATGCTAGCTACTGGTACTACCCTAGCCACAGCCATTAGTTCCATTAAAAAGAACCACCCGATTAAGATTAGTGTGGTGGCCATAGTAGCAGCACCAGAGGGCATTAAAGCCGTGGAAGCAGCGCACCCTGATGTGGACATTTACCTAGCAGCCATTGATGAAAAATTAAATAAGGATAATTACATTATTCCCGGGCTTGGTGATGCTGGGGATCGCTTATTTGGTACTAAATAA", "aa": "MIKKVHHALIENELTKLRDKSSSSSQFRMALSQITSLLFFEVTKESPLEEIVVKTPFTKTKGYKLKNEIVLVPIMRAGLGMVDPIVRYSEKIRVGHLGIYREHEGTNVISYYKKMPENIGNSHVIILDPMLATGTTLATAISSIKKNHPIKISVVAIVAAPEGIKAVEAAHPDVDIYLAAIDEKLNKDNYIIPGLGDAGDRLFGTK", "aa_hexdigest": "92a11133979695b8a841da5a0124591c", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137D2C", "ncbi_nrp_id": "WP_010874390.1", "uniref100_id": "UniRef100_P75081", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137D2C", "RefSeq:WP_010874390.1", "UniRef:UniRef100_P75081" ] }, "ips": { "uniref100_id": "UniRef100_P75081", "uniref90_id": "UniRef90_P75081" }, "psc": { "uniref90_id": "UniRef90_P75081", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_P47276", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_P47276", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47276" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "CNOCLNGIAL_106", "locus": "GCA000143945_00066" }, { "type": "cds", "sequence": "contig_1", "start": 36785, "stop": 37246, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12836.13", "PFAM:PF14579.12" ], "nt": "TTGAGCTACTATGCTTGTTTGTTGAGCTTTAAACTCAAGGAACATGATGTCAGTGGTTTTAAATCAGGTGTGAGCTTTGTCAAGCAAAAACTAGAGGAGCTCAACACACTGTACCGCATTAAACGGATTAAACCCAAAGAAGCAGAACTGTTAACCAGTTACGAGGTTTATTTAGAGATGATGGCCCGCGGCATTAAACTAGAACAAATCTCTTTAACCCACTCCCACGCCACTCGTTTTGTCGAGCACAACGGCATGCTAATTGCCCCTTTTATTACGATTCCGGGGATGGGGGAAGCTGTGGCCAATTCGATCATAGAAGCGCGTAACGAAAAGCCCTTTAGTTCGCTCGATGACTTTAAAAAACGCACTAAAATTACCAAAAAACACATTGAAGCGTTTACCCAAATGCAACTACTCGATGAGTTTAGGGAGCAAGACAACCAGAAAAAACTTTTTTAG", "aa": "MSYYACLLSFKLKEHDVSGFKSGVSFVKQKLEELNTLYRIKRIKPKEAELLTSYEVYLEMMARGIKLEQISLTHSHATRFVEHNGMLIAPFITIPGMGEAVANSIIEARNEKPFSSLDDFKKRTKITKKHIEAFTQMQLLDEFREQDNQKKLF", "aa_hexdigest": "e85c6b3db54daca18ee297e049f7f416", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12836.13", "name": "HHH_3", "length": 42, "aa_cov": 0.2679738562091503, "hmm_cov": 1.0, "evalue": 3.0720614101067383e-09, "score": 32.35352325439453, "start": 93, "stop": 133 }, { "id": "PF14579.12", "name": "HHH_6", "length": 87, "aa_cov": 0.5424836601307189, "hmm_cov": 0.9885057471264368, "evalue": 6.403742382790091e-16, "score": 53.87648010253906, "start": 64, "stop": 146 } ], "seq_stats": { "molecular_weight": 17792.491299999987, "isoelectric_point": 9.097536659240724 }, "id": "CNOCLNGIAL_107", "locus": "GCA000143945_00067" }, { "type": "cds", "sequence": "contig_1", "start": 37277, "stop": 37810, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGTTAAAGCACCTGGCCGATCTTACCCATGTCAATCCACAAAACATTCCCCGGTTTGACAAGAAGCTCACCGAAATGTTTTGGTCGGTCAATCCCTTAAAACTAAAACCGCACTACTTAGATGAACCCACGGGAGCGATTGGGATTCCCGAGTTTGGTACTAAGTTCGTTCGCAAGATCTTGGAACAAACCAAACCAAAGGGCTTTGGTGACCTCATTCGCGTCTCGGGTCTAAGTCACGGGAAGAACGTGTGGGCGGACAATGCGCAGAAAATCTTGAAGGACCAAAACTTAAGCTTAAAGGATGTAATTGCCTGTCGCGATGACATTATGCTCTACCTCATTCATAAGGGGATGCAGGCCAAAGATGCTTTTGAGATCATGGAAAAGGTGCGTAAGGGAATTGCTCTCAATGCCAAGGAAGTGCAATTAATGCAGAGCAATGGGGTCGAACAACACTGGATTAATTCGTGTTTAAAGATTAGCTACCTGTTCCCGAAAGCCCACGCCGCGGCTTATGTTTTAATGGCCTGA", "aa": "MLKHLADLTHVNPQNIPRFDKKLTEMFWSVNPLKLKPHYLDEPTGAIGIPEFGTKFVRKILEQTKPKGFGDLIRVSGLSHGKNVWADNAQKILKDQNLSLKDVIACRDDIMLYLIHKGMQAKDAFEIMEKVRKGIALNAKEVQLMQSNGVEQHWINSCLKISYLFPKAHAAAYVLMA", "aa_hexdigest": "8fa18bb424c0300ae655adab1ba74e3c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 162, "aa_cov": 0.7853107344632768, "hmm_cov": 0.9135802469135802, "evalue": 1.164342847294264e-25, "score": 85.28016662597656, "start": 2, "stop": 140 } ], "seq_stats": { "molecular_weight": 20107.401399999995, "isoelectric_point": 9.373783683776853 }, "id": "CNOCLNGIAL_108", "locus": "GCA000143945_00068" }, { "type": "cds", "sequence": "contig_1", "start": 37889, "stop": 38815, "strand": "-", "frame": 2, "gene": null, "product": "DNA polymerase III alpha subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W4V224" ], "nt": "GTGTTTGTGCGTACCACAGGGGAAATGCTCAATGAAATGAGTTTTTTAGGCGAACAATTAGCTTACGAGTTAGTGGTCAAAAACACCAATAAGTTAGCGAAACAACTCACCGCTGATGACCTGGTACCAGTGCAAACCAAACTCCAACCACCAGTAATTGAAGGTTCTAACGAAAACCTGGCAGCCAAAACCTGGAGTCAAGCCAAGGCCATTTACGGTGATCCCTTACCTAAGTTAATAGAACAACGGATCCAGGAAGAGTTAAAAGCGATCATTGACAATGGCTTTGGCATTATTTACTGGATTTCCCATTTACTAGTCAAACAATCGGTTCAAGATGGTTACTTTGTTGGACCGCGGGGTTCAATTGGTTCTTCGTTGGTAGCTAACCTGATTGGTATCTCGGAAATTAATCCCTTAGTGCCGCATTATCTCTGTGAGAGCTGTCAGTACTTTGAGGTCAACGAAGAGGTCGATGATGGGTATGACCTCATGGTAAGGGATTGTCCAAAGTGTGGTGCCAAAGCAGCCTTTAAGGGTGATGGTCATAACATTCCCTTTGCTACCTTTATGGGTTTTGCTGGGGACAAGATTCCCGATATTGACTTAAACTTTTCCAGTGAGTACCAAGCCAAAGCCCATGCTTATGTGAGGGAACTGTTTGGTGAACAGTACACCTTCCGTGCTGGCACAATTGCGACAGTGGCCGAAAAAACGGCTTACGGGTATGCGCGCAACTACTTTGAAATTATTAAACAAACCGAATTGGCCACCGCTCCCGAAATTGAACGCTTTAAGCAAAAGTTAGTCGGGATTAAACGCACGACCGGCCAACACCCCGGGGGGATTATGATCTTCCCCAACCACAAATCGGTATATGAGTTTACCCCGTGTGGTTATCCAGCTGATGATACTTCCAGTGATTGA", "aa": "MFVRTTGEMLNEMSFLGEQLAYELVVKNTNKLAKQLTADDLVPVQTKLQPPVIEGSNENLAAKTWSQAKAIYGDPLPKLIEQRIQEELKAIIDNGFGIIYWISHLLVKQSVQDGYFVGPRGSIGSSLVANLIGISEINPLVPHYLCESCQYFEVNEEVDDGYDLMVRDCPKCGAKAAFKGDGHNIPFATFMGFAGDKIPDIDLNFSSEYQAKAHAYVRELFGEQYTFRAGTIATVAEKTAYGYARNYFEIIKQTELATAPEIERFKQKLVGIKRTTGQHPGGIMIFPNHKSVYEFTPCGYPADDTSSD", "aa_hexdigest": "5fad5e154f3144b580c139003257883a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_W4V224", "query_cov": 0.8798701298701299, "subject_cov": 0.8187311178247734, "identity": 0.513, "score": 292.0, "evalue": 1.1e-94, "valid": false, "product": "DNA polymerase III alpha subunit", "uniref50_id": "UniRef50_W4V224" }, "pscc": { "uniref50_id": "UniRef50_W4V224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W4V224" ], "product": "DNA polymerase III alpha subunit" }, "genes": [], "id": "CNOCLNGIAL_109", "locus": "GCA000143945_00069" }, { "type": "cds", "sequence": "contig_1", "start": 38861, "stop": 39652, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGGTAGCCCATAACGGTTTGAGTTTTGACTTGCCCTTTTTACAAACCCAGTTTGAAAAATACAACATTGCGCCACTAACCAATCCCTTAATTGATACCTTAGCCTTATCCTGGGCCTTAAATCCCGGGTTTGCCTCCCATACGTTGAGCAATATTTGTGCCAAGCTCAAGTTTGACTTTGATGATGAACGGTTGCACCGAGCGGATTATGATACCCAGGCCTTAAAGAAGGTCTTTGACTACTTCAAGGAACAGGTCGAACTCATGGGGATTACTAATCTCGAGCAGTTAGACCAAGAGTTAAACCAGCAGTGCCACTTTGAACTGCTCAAGCGCACTTTTACCAATACCGGCATTATCTACATTAAGAGCCAAAGTGGGTTTGCTAAACTGTATGAACTGTTATCAATTGCGCTCACCGATAACAACGCTACTCGACCCCTAGTGTTAACTTCAACACTCCAAAAGTTTGCTAAATCCTTTGTCATTACTGATAACCCGGTGCAAGGTGATATCTTTAAAGCGGCATTGACTAAACCGCTTAAAGAATTAGAAGCGGCCATTAAAAGAGTTGACTTTGTTTTAATTGCCCCACCTGATGCTTATGCTGGTTACACCATCAGGGAAGGTTTGAAAAAAGAAGCCATTCCTAACGCAATTAAGTTAGTGGTGGACACGGCCCAAAGACTCAATAAGTTAGTGGCAGTTGCCTCGGATGCTTACTTTATTCACCCCTGGGAGAATGAGTACTATAAGGCCATAGTTTGTGCCAAGGGTCTGGGTGGGCGTTGA", "aa": "MVAHNGLSFDLPFLQTQFEKYNIAPLTNPLIDTLALSWALNPGFASHTLSNICAKLKFDFDDERLHRADYDTQALKKVFDYFKEQVELMGITNLEQLDQELNQQCHFELLKRTFTNTGIIYIKSQSGFAKLYELLSIALTDNNATRPLVLTSTLQKFAKSFVITDNPVQGDIFKAALTKPLKELEAAIKRVDFVLIAPPDAYAGYTIREGLKKEAIPNAIKLVVDTAQRLNKLVAVASDAYFIHPWENEYYKAIVCAKGLGGR", "aa_hexdigest": "1dcf9f2dca7f7c2681c23bba5e70d085", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 65, "aa_cov": 0.24714828897338403, "hmm_cov": 0.9538461538461539, "evalue": 1.0335383865157487e-06, "score": 24.250858306884766, "start": 1, "stop": 65 }, { "id": "PF00929.31", "name": "RNase_T", "length": 81, "aa_cov": 0.30038022813688214, "hmm_cov": 0.9629629629629629, "evalue": 2.287624541542636e-15, "score": 52.992740631103516, "start": 1, "stop": 79 } ], "seq_stats": { "molecular_weight": 29623.76490000001, "isoelectric_point": 6.528208351135253 }, "id": "CNOCLNGIAL_110", "locus": "GCA000143945_00070" }, { "type": "cds", "sequence": "contig_1", "start": 39668, "stop": 40129, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00929.31" ], "nt": "ATGAGTGCCTTTGATGGCATTAACAGTGTCCAGGAATACGCCCAGTTTGCCAAAGAACGCGATTGGAAGACAATTGCTGTCACTGACAAGGACAACATTCACATTTATCCCACTTTATATGAAGTGGCCAAAAAGTACGGCTTGAAGGCCATTTATGGTTTGGAATGTAACCTCATTGATGATCACATTAAGATAGTTAGCAATCCTGATAAAACCAAACTTAAGGATGCGACCTTTGTCATCTTCGACATTGAAACAACTGGGTTACACGGTCGGTATGACTCGGTGATTGAGTTTGCGGGAATTAAGGTCAAACATAACCGTGAAGTGGAACGAATGCAGTTCTTTCTCAAGATTGATGGACCCTTACCTGCAGCAGTTACCGAAATTACCAAGATTACCCAAGCACAACTAGAAGATGGGATGGAACAGCAAGCGGGGTTGGAAAAACTGCGCGCTTGA", "aa": "MSAFDGINSVQEYAQFAKERDWKTIAVTDKDNIHIYPTLYEVAKKYGLKAIYGLECNLIDDHIKIVSNPDKTKLKDATFVIFDIETTGLHGRYDSVIEFAGIKVKHNREVERMQFFLKIDGPLPAAVTEITKITQAQLEDGMEQQAGLEKLRA", "aa_hexdigest": "d2a265eba449a44e251e5e808abb06eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00929.31", "name": "RNase_T", "length": 77, "aa_cov": 0.47058823529411764, "hmm_cov": 1.0, "evalue": 5.1349687541031404e-17, "score": 58.354957580566406, "start": 80, "stop": 151 } ], "seq_stats": { "molecular_weight": 17379.67629999998, "isoelectric_point": 5.581043434143066 }, "id": "CNOCLNGIAL_111", "locus": "GCA000143945_00071" }, { "type": "cds", "sequence": "contig_1", "start": 40280, "stop": 40888, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAGGGGCAGAACTGAGGATTACCTTCCAAGAAGATGCCACCTTCTTTAAGGACGGGTCCACTAAGTCCAGTGTCACCTTAGCCATTATCAAAGACTACTTCAAAAGCTTCTTTGGTAAGGACAAGAAGTACCGCATCCTGTTAGAGCAAGAGTTAACCCATCCTAACTTCTTGTCCTACAGTAACCATGAACTGAAAGCTAACTGCCAGTCCCAAGAATTAGACCAATGGTTAATTGAACAACGCCAAGCCTTCATTAAGTGGATGCACCAAGCTGGGTTTACCCACTTTGGTTTTGTCTCGTTGTTTAATCCACCAGCGGAAAAACAACTAAAAGTGAAATCAATGAAGGTATCCAAGTACGACAAGCAGTTTGAAACCGAGGTCTTTAGTACGGAATTTGTCCCCATTCACAAGATTAACCAACAAATGGATGAGATCAAACTAATGGGGCAAATCTTTGAGTTAAAGGACTTTCCTGGGTACAACAACCTCCGTAATACCCTTAATATTTATGTCACTGATTTCCAACTAGGTGGTTCGTTAATTTTGAAGTGGTTTTACAAGGATCCCAAAACAATTGAGGGCATTAAGATTGGTACCTGA", "aa": "MEGAELRITFQEDATFFKDGSTKSSVTLAIIKDYFKSFFGKDKKYRILLEQELTHPNFLSYSNHELKANCQSQELDQWLIEQRQAFIKWMHQAGFTHFGFVSLFNPPAEKQLKVKSMKVSKYDKQFETEVFSTEFVPIHKINQQMDEIKLMGQIFELKDFPGYNNLRNTLNIYVTDFQLGGSLILKWFYKDPKTIEGIKIGT", "aa_hexdigest": "3282a0396ec0ece36a1a23b3d7268cd1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23706.92160000001, "isoelectric_point": 7.778513526916504 }, "id": "CNOCLNGIAL_112", "locus": "GCA000143945_00072" }, { "type": "cds", "sequence": "contig_1", "start": 40916, "stop": 41116, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTCGACACCGAAACCGAAAAGGGTAAACGCATTTTAGCATTATCACAGTTTTTAGTTAAGAAAAATATTCTCGATCACAACGAATTGCACCAACTCAACAACCGGATTGAACTGGTTTACCTCGAGAATGATCGCGAAAACGCCCTGTTTCTCGTGGCGCTCACTTTAAAAAAACCGTTAACAATTGACATTTGA", "aa": "MVFDTETEKGKRILALSQFLVKKNILDHNELHQLNNRIELVYLENDRENALFLVALTLKKPLTIDI", "aa_hexdigest": "0ae06d94c8d77533384386b26ca26e00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7733.936899999996, "isoelectric_point": 6.054341697692871 }, "id": "CNOCLNGIAL_113", "locus": "GCA000143945_00073" }, { "type": "cds", "sequence": "contig_1", "start": 41394, "stop": 42545, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_035", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078", "UniRef:UniRef90_P75079" ], "nt": "ATGAAAGCGTTCCGAAAATTGTTGCTGGGTCTCGCCTTACCCACAACCATCGGTCCCTTATTAGGCTTATTATTAACTAATACTGATACTGTCAAAAATGAAAGCTTAACTACTGTGCGCCACAGGTCCAGCATTAACCAGGACTTTGATGGCATTTTTCATACCGTTAAGTTATTAGAACCAATCCAGCAAAGTAATGCTGATCCCGCCGCTAGTTTTGCGCTCTTTCAGCAAAAGTTTCCCAACCTAAAACGTGTAGCCAACTCCACCTTAAATACCTTTGATGTCTACAACCTTTTGAGTGGTTGGAAGAGTAGCTTAACAGCGTACCTCAACCAGGTGTTGGCACTCCAACAACGGATTAAAGCAGCTGATCAAATTTTCCCTAACCAAAAGGAAACTTTACCCAAGGATGAGAATCCCAACATCTTTGAAGTGTTAGGTGCATATGGTGGTAAGGGTTTCTTTCCCACGCTAGGATCCAACGGGTTACACCTGCCACCACAGCTGTTCCAGTTCTTCCGTGACTTCCAACTAAGTAGCTTTACCATTAAAGACTTTGAGGTAGCGTTAGTGAGTGAACCGGACATAGTGCAACATGACAAAGTGCGCTATGCCTTTCAGGTGCAATTTAACTTAGTGTTACAATTACAAATTAACCGTAACCCAGTGTTATTTGACTTTAACCTACAGTTAAAGACGAATAACTTTGCCAATCAAACCAGTTTTGATGAATTGTTTAACGAAAAAACTAATCCACTTAACTGGCAGTTCTTTAGCAAGCTTAAAGTTAACCATTTAGTGTATGAAGGCAATGACATAACTCAGTTAGCTAATACCTTATTACAAAGCCAGTTTAATGTCTTACAACTTGATTTAAATAAGTCAATTTACCGCTTGAACTTAAACGCAATGGCACAGCGCTTTGAACACGACTGGGTACAACCGTTGTATGCCAAACGCACGCAGGCCAAAATTGCTTACGAAGCGGAACAAGCACGGATTGCGGCCGAGAACAAACGCAAGGAACTAGAACGGCAAAAGCTGTTAGCAGAATTGAAAGCCAAAGCTGAGCATTACCAAAAGATCAAGCAAGCACGGGAAAACATGCTAAAGGGCTTAAAGAGTATTACTGATTTTGTCAAGTTCTGA", "aa": "MKAFRKLLLGLALPTTIGPLLGLLLTNTDTVKNESLTTVRHRSSINQDFDGIFHTVKLLEPIQQSNADPAASFALFQQKFPNLKRVANSTLNTFDVYNLLSGWKSSLTAYLNQVLALQQRIKAADQIFPNQKETLPKDENPNIFEVLGAYGGKGFFPTLGSNGLHLPPQLFQFFRDFQLSSFTIKDFEVALVSEPDIVQHDKVRYAFQVQFNLVLQLQINRNPVLFDFNLQLKTNNFANQTSFDELFNEKTNPLNWQFFSKLKVNHLVYEGNDITQLANTLLQSQFNVLQLDLNKSIYRLNLNAMAQRFEHDWVQPLYAKRTQAKIAYEAEQARIAAENKRKELERQKLLAELKAKAEHYQKIKQARENMLKGLKSITDFVKF", "aa_hexdigest": "47b06f7267b664b8cc264cc9e25d3c55", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 41394, "stop": 42848, "observations": { "mutation": [ 42543, 42651, 42717 ], "stop": [ 42543, 42651, 42717 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75079", "query_cov": 1.0, "subject_cov": 0.575075075075075, "identity": 1.0, "score": 740.0, "evalue": 5.56e-265, "gene_start": 1, "gene_end": 383, "reference_start": 1, "reference_end": 383, "reference_sequence": "MKAFRKLLLGLALPTTIGPLLGLLLTNTDTVKNESLTTVRHRSSINQDFDGIFHTVKLLEPIQQSNADPAASFALFQQKFPNLKRVANSTLNTFDVYNLLSGWKSSLTAYLNQVLALQQRIKAADQIFPNQKETLPKDENPNIFEVLGAYGGKGFFPTLGSNGLHLPPQLFQFFRDFQLSSFTIKDFEVALVSEPDIVQHDKVRYAFQVQFNLVLQLQINRNPVLFDFNLQLKTNNFANQTSFDELFNEKTNPLNWQFFSKLKVNHLVYEGNDITQLANTLLQSQFNVLQLDLNKSIYRLNLNAMAQRFEHDWVQPLYAKRTQAKIAYEAEQARIAAENKRKELERQKLLAELKAKAEHYQKIKQARENMLKGLKSITDFVKFWKSPDRLLVGFNKQDDITTRAGVYKALQIAYANYPQWTFYLTLQGWKNGSELLLKRPSWTNLLSDIHFQKAFNLKNTVSEQTLGAATLPGYGYYGLRMSDWLRWALGYYSYIHMGVPQNVQTKFTGTPDNEQQVWIANEPFEWNKHYGVGPKYKDKAYRFNMEISFELEGWIAVKWWAWAFKGSIPGNWKGKLKVTHLFDGMVPVWELGPVNTHLPQYSFTDQQQLLFVPHSIQKIEAIGADKGINDLLKTQNLHNLERLSYESTNPIDLISYLLYAIQYIKV" }, "paralog": false, "identity": 0.993801652892562, "score": 952.0, "evalue": 0.0, "description": "Internal stop codon at 42543, 42651, 42717. Nonsense mutation around 42543, 42651, 42717.", "psc": { "uniref90_id": "UniRef90_P75079", "product": "Uncharacterized protein MPN_035", "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75079", "UniRef:UniRef50_P75078" ] }, "pscc": { "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078" ], "product": "Uncharacterized protein MPN_036" } }, "id": "CNOCLNGIAL_114", "locus": "GCA000143945_00074" }, { "type": "cds", "sequence": "contig_1", "start": 42834, "stop": 43055, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAGTGACTGGTTGCGCTGGGCTTTGGGCTATTACTCCTATATCCATATGGGCGTACCGCAAAATGTACAGACCAAGTTTACGGGCACCCCAGATAATGAACAACAAGTCTGGATTGCTAACGAACCGTTTGAATGGAACAAACATTACGGTGTTGGTCCTAAATACAAGGATAAGGCCTACCGCTTTAACATGGAAATTAGTTTTGAACTAGAGGGTTGA", "aa": "MSDWLRWALGYYSYIHMGVPQNVQTKFTGTPDNEQQVWIANEPFEWNKHYGVGPKYKDKAYRFNMEISFELEG", "aa_hexdigest": "84fb77351fc69cd429b6d2c85d627e64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 69, "aa_cov": 0.9452054794520548, "hmm_cov": 0.9420289855072463, "evalue": 3.8386126180088385e-19, "score": 64.58252716064453, "start": 5, "stop": 73 } ], "seq_stats": { "molecular_weight": 8738.680300000002, "isoelectric_point": 5.5347196578979485 }, "id": "CNOCLNGIAL_115", "locus": "GCA000143945_00075" }, { "type": "cds", "sequence": "contig_1", "start": 43146, "stop": 43394, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGGTACCAGTGTGGGAATTGGGCCCAGTCAATACCCATTTACCCCAATACAGCTTTACCGATCAGCAACAACTGTTGTTTGTACCCCACTCCATTCAAAAGATTGAAGCGATTGGTGCGGACAAAGGTATAAACGATCTTTTAAAAACCCAAAACCTCCATAACCTAGAGCGCTTGTCATACGAATCAACTAACCCAATTGACCTCATTTCTTACCTCTTGTACGCGATCCAGTACATTAAGGTTTAG", "aa": "MVPVWELGPVNTHLPQYSFTDQQQLLFVPHSIQKIEAIGADKGINDLLKTQNLHNLERLSYESTNPIDLISYLLYAIQYIKV", "aa_hexdigest": "7edf4e6e1b6f8935ebc8151ddee24830", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 36, "aa_cov": 0.43902439024390244, "hmm_cov": 1.0, "evalue": 6.736072839475043e-12, "score": 41.1209716796875, "start": 2, "stop": 37 } ], "seq_stats": { "molecular_weight": 9438.723299999994, "isoelectric_point": 5.375115776062012 }, "id": "CNOCLNGIAL_116", "locus": "GCA000143945_00076" }, { "type": "cds", "sequence": "contig_1", "start": 43566, "stop": 43877, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGAAAACACTGCACAAGTTGCTGTTGGGGCTTTCCTTACCAGCAATCCTCGGTCCGTTGTTTGGTCTACTAGTTACAAAAACTGATGCTGAATCACCAGTTGCAGCATTACATAAATCGAAATCTTTTAGCAAAAACTTAGATAGTTTCCGCGCCTTATTTGTGGGCGAGGATATTGAGGAACAACTAGCTAAGGTACAACAACAAACACCAGAAGTTAGCTTTGCTAGCTTTCAACAAAAGTTTCCTGATAAGGCCAGTTTAAGAAACGGTTTTCAACCAATTGATGTTTACAACTTTTTAAGTGGTTGA", "aa": "MKTLHKLLLGLSLPAILGPLFGLLVTKTDAESPVAALHKSKSFSKNLDSFRALFVGEDIEEQLAKVQQQTPEVSFASFQQKFPDKASLRNGFQPIDVYNFLSG", "aa_hexdigest": "c35a577a259b83e413296f3984acc7c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 40, "aa_cov": 0.3786407766990291, "hmm_cov": 1.0, "evalue": 1.56531521258578e-11, "score": 39.49834060668945, "start": 65, "stop": 103 } ], "seq_stats": { "molecular_weight": 11350.95979999999, "isoelectric_point": 8.027168083190919 }, "id": "CNOCLNGIAL_117", "locus": "GCA000143945_00077" }, { "type": "cds", "sequence": "contig_1", "start": 43899, "stop": 44339, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "TTGGCTAAGGTAGTTGAACTGCAAAAGAAGATTAAAGCAGCTGACGATATTTTCCCGAACCAAAAGAAAAATCCCGACAAGAAAGATAACCCAAATGTTTTAGAAGTGTTAGGTGAATACGGTGGAGATGGTTTCTTTCCTACATTAGGTAATAACGGTTTAAACATCCCGGACCAAGTTTTCCAATCCTTTAATAACTTCCAAATTGAGAACTATAAGATTAGTGACTTTAAGGTGGACATTGCCAGTGAACGTGACATTGTCCAACACGACAAGTTCCGTTTTAGTTATGTCGTTAACATTGGTTTAGAGTTAGCACTTTTAGTCAATAAGCGTCCGGTTTACTTTAACTTTAGCTTGGATCTGCGGACCAATAACTTCTCCAACCAAGCCGGTTTTAACGAAATCTTTAATGCGCCTGGTAACGCAGCTACTAACTGA", "aa": "MAKVVELQKKIKAADDIFPNQKKNPDKKDNPNVLEVLGEYGGDGFFPTLGNNGLNIPDQVFQSFNNFQIENYKISDFKVDIASERDIVQHDKFRFSYVVNIGLELALLVNKRPVYFNFSLDLRTNNFSNQAGFNEIFNAPGNAATN", "aa_hexdigest": "e7988e96cf7e736d18cf425a3e5c8a27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 143, "aa_cov": 0.9794520547945206, "hmm_cov": 1.0, "evalue": 1.722961018780146e-66, "score": 219.39715576171875, "start": 2, "stop": 144 } ], "seq_stats": { "molecular_weight": 16523.340499999988, "isoelectric_point": 5.385744667053221 }, "id": "CNOCLNGIAL_118", "locus": "GCA000143945_00078" }, { "type": "cds", "sequence": "contig_1", "start": 44361, "stop": 44723, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "GTGAAACAGTTGAACTATGATGGTAATGACAGTACCCATTTAGCCAATACCCTCTTACAAGATCAGTTCAATACCTTAAACTTAAACTTGCAAAAGTCGATCTATGAGTTGCCATTAACCGAAATGGAAACTAAGTTCCAGCAAGAATATGTGGAACTTTTACTAGCTAAACGTAGAGAGGAAAAACGACTTTGGGATGAAGAACAAAAACGAATTGAAGCAGAACGGAAACAAAAAGAAGCAGAGCTAGCACAAATTCAGCGTGAACTTAAAGAAAAGGCTGAGAAGGATGCGGCGGTTAAACAAGCTCAAACTAACTTAAAAACTGCTCTAAGTAGTGTGGAAGGCTTTGTGAAATTCTGA", "aa": "MKQLNYDGNDSTHLANTLLQDQFNTLNLNLQKSIYELPLTEMETKFQQEYVELLLAKRREEKRLWDEEQKRIEAERKQKEAELAQIQRELKEKAEKDAAVKQAQTNLKTALSSVEGFVKF", "aa_hexdigest": "1a8d0ee571f925de26a377aeb83b8535", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 34, "aa_cov": 0.2833333333333333, "hmm_cov": 1.0, "evalue": 9.667055079547111e-14, "score": 46.73021697998047, "start": 2, "stop": 35 } ], "seq_stats": { "molecular_weight": 14157.850599999985, "isoelectric_point": 5.581100273132324 }, "id": "CNOCLNGIAL_119", "locus": "GCA000143945_00079" }, { "type": "cds", "sequence": "contig_1", "start": 45081, "stop": 45254, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "GTGAAAGCCACCATTGATGGTAATCCAAGTGATACCACCAAAGTATGGATTGCACCAGAAGACTTTAAACAGGGTGTACACATTGGTGTGGGGGAACACTTCAAAGGTAAGGCTTACAAGTTTAAGTTTGAAGTTAGTTTTGAATTAGAAGGCTCGATTGCAGCCCACTGGTGA", "aa": "MKATIDGNPSDTTKVWIAPEDFKQGVHIGVGEHFKGKAYKFKFEVSFELEGSIAAHW", "aa_hexdigest": "cc0b7608021588bb904448ec69e0ed11", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 56, "aa_cov": 0.9824561403508771, "hmm_cov": 0.9821428571428571, "evalue": 2.5723623808348135e-15, "score": 52.1909294128418, "start": 2, "stop": 57 } ], "seq_stats": { "molecular_weight": 6381.144599999998, "isoelectric_point": 6.247423744201661 }, "id": "CNOCLNGIAL_120", "locus": "GCA000143945_00080" }, { "type": "cds", "sequence": "contig_1", "start": 45721, "stop": 45996, "strand": "-", "frame": 3, "gene": null, "product": "DUF16 family-like protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031ACD5D", "UniRef:UniRef100_A0AB38W8A6", "UniRef:UniRef50_A0AB38W8A6", "UniRef:UniRef90_A0AB38W8A6" ], "nt": "GTGAACACTGAATACGTAACGCGTAAAGAGTTTAACGAGTACAAAAAATCAAAAGACCAACGACTTACAAAGATTGAAACTGAGTTAAAGAGTCAGGGTCAAAGGATTCAAGTTGTTGAGGACAAAGTTGATCGACTAACTGAAGTTGTTATCGTTCAAGGCCAACAAATTAAAGAGCTTCAAGTTGAACAAAAAAATCAAGGAGAAACACTTAGATTAATTTTGAAAGCACTTGAAGGCATAAACAGACGTCTAGACAATTTGGAATCTAAATAA", "aa": "MNTEYVTRKEFNEYKKSKDQRLTKIETELKSQGQRIQVVEDKVDRLTEVVIVQGQQIKELQVEQKNQGETLRLILKALEGINRRLDNLESK", "aa_hexdigest": "02da2a5cb0267e820093654141b6962d", "start_type": "GTG", "rbs_motif": "AACAA", "ups": { "uniparc_id": "UPI00031ACD5D", "uniref100_id": "UniRef100_A0AB38W8A6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031ACD5D", "UniRef:UniRef100_A0AB38W8A6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8A6", "uniref90_id": "UniRef90_A0AB38W8A6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8A6", "product": "DUF16 family-like protein", "uniref50_id": "UniRef50_A0AB38W8A6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6" ], "product": "DUF16 family-like protein" }, "genes": [], "id": "CNOCLNGIAL_121", "locus": "GCA000143945_00081" }, { "type": "cds", "sequence": "contig_1", "start": 45993, "stop": 46163, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8L9", "UniRef:UniRef90_A0AB38W8L9" ], "nt": "ATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTTCATGACATGGTGAAAAAGACCAAAAGGGTACTTTTTCAGGTTGATATATTATCGACAAAGACAACAACAGTGTGGAATTCTCTGGCAATTTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGA", "aa": "MKEKIPFYNEKEFHDMVKKTKRVLFQVDILSTKTTTVWNSLAISTDNLNLTNRLYQ", "aa_hexdigest": "a2adc6dc26cef7ab1b79e31810ec82d6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W8L9", "query_cov": 1.0, "subject_cov": 0.9180327868852459, "identity": 1.0, "score": 109.0, "evalue": 1.45e-30, "valid": true, "uniref50_id": "UniRef50_A0AB38W8L9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8L9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8L9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6692.6721, "isoelectric_point": 9.455400657653808 }, "id": "CNOCLNGIAL_122", "locus": "GCA000143945_00082" }, { "type": "cds", "sequence": "contig_1", "start": 46427, "stop": 46777, "strand": "-", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_038", "db_xrefs": [ "RefSeq:WP_010874395.1", "SO:0001217", "UniParc:UPI0000139F43", "UniRef:UniRef100_P75076", "UniRef:UniRef50_P75076", "UniRef:UniRef90_P75076" ], "nt": "TTGTTTAAGAAAAGACTAAATAAAGATAAGATTAATGATTGTTATACATGGGAAGAAGAACTACCTGATGGTAGCTACGACATGGGATTTCATGGCAATTTAAACCATATGGAAAAAGGTAAAAGTGGATACGTCACCCATAAACAACTGGATAAAAAACTAGAAGTGTTCAAACAAGATCTTCTAGTTGAACTTAGTGAAAAATTTGTAACTAAGGAAGAATTCAGAGCTCAGGGCAAGCAAATCAAAGAACTTCAAATTGAGCAAAAGGCTCAAGGCAAGACACTTCAATTAATACTCGAAGCTCTCCAGGGTATAAACAAACGTTTAGACAAATTGGAATCTAAGTAG", "aa": "MFKKRLNKDKINDCYTWEEELPDGSYDMGFHGNLNHMEKGKSGYVTHKQLDKKLEVFKQDLLVELSEKFVTKEEFRAQGKQIKELQIEQKAQGKTLQLILEALQGINKRLDKLESK", "aa_hexdigest": "a093b243fb02ab51931a319ab54c7036", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139F43", "ncbi_nrp_id": "WP_010874395.1", "uniref100_id": "UniRef100_P75076", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139F43", "RefSeq:WP_010874395.1", "UniRef:UniRef100_P75076" ] }, "ips": { "uniref100_id": "UniRef100_P75076", "uniref90_id": "UniRef90_P75076" }, "psc": { "uniref90_id": "UniRef90_P75076", "product": "UPF0134 protein MPN_038", "uniref50_id": "UniRef50_P75076" }, "pscc": { "uniref50_id": "UniRef50_P75076", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75076" ], "product": "UPF0134 protein MPN_038" }, "genes": [], "id": "CNOCLNGIAL_123", "locus": "GCA000143945_00083" }, { "type": "cds", "sequence": "contig_1", "start": 47179, "stop": 47499, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AD341A4", "UniRef:UniRef50_UPI001FCF25EA", "UniRef:UniRef90_UPI000AD341A4", "UniRef:UniRef90_UPI001FCF25EA", "PFAM:PF03086.20" ], "nt": "ATGACAAAACTCCCTAAATTGTTACTGGGGTTAACCTTTTCTGTCTCACTTATACCGTTTTCTAGTTTGCTTATTACTAGTACTGATGTCAATAAGCAACCGGTTCCAACTGCCTTACAACGCACTGGCGCCTCTGCCATTCATGAAGGTAGTTTTCAAACTATTACTTTAGGTCAAAGTTTAATGGAGCAAATTGAACAGCTCCAACAATTTACGCCAGCGCAGCGCTTTACCCAATTTAAAAAGAAATTTCCCAACCAAAAATTACTCAGTCAAAGTGAGTTAAGTCCAGTTGATGTTTACAATTTTTTAAGTGGTTGA", "aa": "MTKLPKLLLGLTFSVSLIPFSSLLITSTDVNKQPVPTALQRTGASAIHEGSFQTITLGQSLMEQIEQLQQFTPAQRFTQFKKKFPNQKLLSQSELSPVDVYNFLSG", "aa_hexdigest": "a54d492bede0e0f831adf1662ae286f0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCF25EA", "query_cov": 0.8207547169811321, "subject_cov": 0.925531914893617, "identity": 1.0, "score": 162.0, "evalue": 5.25e-50, "valid": true, "uniref50_id": "UniRef50_UPI001FCF25EA" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCF25EA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCF25EA" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 47179, "stop": 47737, "observations": { "insertion": [ 47713 ], "mutation": [ 47497 ], "stop": [ 47497 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AD341A4", "query_cov": 1.0, "subject_cov": 0.572972972972973, "identity": 1.0, "score": 198.0, "evalue": 3.19e-63, "gene_start": 1, "gene_end": 106, "reference_start": 1, "reference_end": 106, "reference_sequence": "MTKLPKLLLGLTFSVSLIPFSSLLITSTDVNKQPVPTALQRTGASAIHEGSFQTITLGQSLMEQIEQLQQFTPAQRFTQFKKKFPNQKLLSQSELSPVDVYNFLSGWQGALVSFLDRVIKLQGKVKEANEIFNPNVGDQIVLPKKENPNVLEVLGEYNGFGFFPTLGKNGLNLPQQIFKTLLTLK" }, "paralog": false, "identity": 0.9945945945945946, "score": 353.0, "evalue": 1.29e-126, "description": "Internal stop codon at 47497. Frameshift due to insertion around 47713. Nonsense mutation around 47497.", "psc": { "uniref90_id": "UniRef90_UPI000AD341A4", "uniref50_id": "UniRef50_UPI000AD341A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AD341A4", "UniRef:UniRef50_UPI000AD341A4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AD341A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AD341A4" ] } }, "hypothetical": true, "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 41, "aa_cov": 0.3867924528301887, "hmm_cov": 1.0, "evalue": 1.442310444377513e-12, "score": 42.88805389404297, "start": 66, "stop": 106 } ], "seq_stats": { "molecular_weight": 11771.48239999999, "isoelectric_point": 9.52193202972412 }, "id": "CNOCLNGIAL_124", "locus": "GCA000143945_00084" }, { "type": "cds", "sequence": "contig_1", "start": 47509, "stop": 47967, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_039", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75075", "UniRef:UniRef90_P75075" ], "nt": "TTGGTCTCTTTCTTGGATAGAGTAATTAAATTACAAGGCAAGGTTAAGGAAGCAAATGAAATTTTTAATCCAAATGTAGGTGATCAAATAGTTTTACCTAAAAAGGAAAACCCGAATGTTTTGGAAGTATTGGGCGAGTATAACGGTTTTGGTTTCTTCCCTACATTGGGTAAAAACGGATTAAACCTACCACAGCAAATCTTTGAAAACTTTACTGACTTTAAAGTAGAGAGTTACCAAATTAACGATTTCAAGGTAAGTCTAGTGGGCGAGCGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAACTGCTTGTTAACAACCAAAAGGTTACTTTCAATATTACGGTTGATTTGAGAACCAATAACTTTTCTACCCAAGAGACTTTCAATGATTTATTCAACAATGGAACTGCACCAACCAACTGA", "aa": "MVSFLDRVIKLQGKVKEANEIFNPNVGDQIVLPKKENPNVLEVLGEYNGFGFFPTLGKNGLNLPQQIFENFTDFKVESYQINDFKVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTN", "aa_hexdigest": "49ae170815a8e828560d47f96ed40d8b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 47209, "stop": 48195, "observations": { "mutation": [ 47497, 47965 ], "stop": [ 47497, 47965 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75075", "query_cov": 1.0, "subject_cov": 0.44970414201183434, "identity": 0.993, "score": 301.0, "evalue": 5.47e-101, "gene_start": 1, "gene_end": 152, "reference_start": 111, "reference_end": 262, "reference_sequence": "MTKLPKLLLGLTFSVSLIPFSSLLITSTDVNKQPVPTALQRTGASAIHEGSFQTITLGQSLMEQIEQLQQFTPAQRFTQFKKKFPNQKLLSQSELSPVDVYNFLSGWQDALVSFLDRVIKLQGKVKEANEIFNPNVGDQIVLPKKENPNVLEVLGEYNGFGFFPTLGKNGLNLPQQIFENFTDFKVESYQINDFKVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTNWQFFSRIKVNKLHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDDLEERFEEEYAKPLREKRTQQKKEWEEE" }, "paralog": false, "identity": 0.9878048780487805, "score": 637.0, "evalue": 8.19e-236, "description": "Internal stop codon at 47497, 47965. Nonsense mutation around 47497, 47965.", "psc": { "uniref90_id": "UniRef90_P75075", "product": "Uncharacterized protein MPN_039", "uniref50_id": "UniRef50_P75075", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75075", "UniRef:UniRef50_P75075" ] }, "pscc": { "uniref50_id": "UniRef50_P75075", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75075" ], "product": "Uncharacterized protein MPN_039" } }, "id": "CNOCLNGIAL_125", "locus": "GCA000143945_00085" }, { "type": "cds", "sequence": "contig_1", "start": 47998, "stop": 48195, "strand": "+", "frame": 1, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF9F4C5", "UniRef:UniRef90_UPI000AF9F4C5" ], "nt": "TTGCACTATGACCAAACTGATGCCACCCATTTAGCTAACACCCTACTACAAGACCAGTTTAATGCGTTAAACCTTGATTTACAAAAATCGATTTACGACCTTAACCTTGATGGTTTAGAAGAAAGGTTTGAAGAGGAATACGCTAAGCCACTTCGTGAAAAAAGAACTCAGCAAAAGAAAGAATGGGAAGAGGAATAG", "aa": "MHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDGLEERFEEEYAKPLREKRTQQKKEWEEE", "aa_hexdigest": "cccfa81d6f589aa24b73ad76951de46e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 47773, "stop": 48195, "observations": { "mutation": [ 47965 ], "stop": [ 47965 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF9F4C5", "query_cov": 1.0, "subject_cov": 0.4642857142857143, "identity": 0.985, "score": 125.0, "evalue": 1.65e-35, "gene_start": 1, "gene_end": 65, "reference_start": 76, "reference_end": 140, "reference_sequence": "MGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTNWQFFSRIKVNKLHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDGLEERFEEEYAKPLREKRTQQKKEWEEE" }, "paralog": false, "identity": 0.9857142857142858, "score": 278.0, "evalue": 1.91e-98, "description": "Internal stop codon at 47965. Nonsense mutation around 47965.", "psc": { "uniref90_id": "UniRef90_UPI000AF9F4C5", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000AF9F4C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF9F4C5", "UniRef:UniRef50_UPI000AF9F4C5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF9F4C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF9F4C5" ], "product": "DUF240 domain-containing protein" } }, "id": "CNOCLNGIAL_126", "locus": "GCA000143945_00086" }, { "type": "cds", "sequence": "contig_1", "start": 48215, "stop": 48379, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAACTGCAAAAAGAAATTGAAGAACAACAAAAGAAACAAAGAGAACAAGCAAAAAACGATGCTGAGCTCAAAAAAGCACAAGATGATCTCAAAGTTGCGCTTAATAGTCTTGACGGTTTTGTTAAATTCTTTACATCAAACGAAGGTCGCTTTAAATTAG", "aa": "MLNCKKKLKNNKRNKENKQKTMLSSKKHKMISKLRLIVLTVLLNSLHQTKVALN", "aa_hexdigest": "736e452a7e7efcc8a81098d493c761a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6329.709699999999, "isoelectric_point": 11.003479957580566 }, "id": "CNOCLNGIAL_127", "locus": "GCA000143945_00087" }, { "type": "cds", "sequence": "contig_1", "start": 48994, "stop": 49215, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "GTGCGTCCACCACAATACAGTTTTAAGGAACAAGAAAAACAACTTTTATTTGTACCACGTCATGTACAAAAAATTGAAGCTGAAGGTAAACACCTTGAAATAATTAATCCGTTCTTAAAAGATCAACATTTAGATTTTTTTGAACACTACCACCCAGATTTAACGCAGCCTTTAGACATGGTGTCATACCTAATGTATGCCATCGCTGACAAGGTGAAATAA", "aa": "MRPPQYSFKEQEKQLLFVPRHVQKIEAEGKHLEIINPFLKDQHLDFFEHYHPDLTQPLDMVSYLMYAIADKVK", "aa_hexdigest": "f62d6569114a02f37a7eac1ee64640fa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 27, "aa_cov": 0.3698630136986301, "hmm_cov": 0.9629629629629629, "evalue": 2.4144138225967765e-07, "score": 26.370834350585938, "start": 2, "stop": 28 } ], "seq_stats": { "molecular_weight": 8811.087799999996, "isoelectric_point": 6.16506404876709 }, "id": "CNOCLNGIAL_128", "locus": "GCA000143945_00088" }, { "type": "cds", "sequence": "contig_1", "start": 49277, "stop": 49582, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGAAAAAACTCCACAAAATTTTATTGGGTTTATCTTTGCCCGCATCTTTGGCGCCCTTATCTGGGTTTATTAGTACTGATGCTGATAACTCACAGTTTTCACTAAAGAAGTCTAGCACTGAAATTAAAGGTACACAATCCATTCACCTGCTTAATTTAGGGGAAAGTTTAACTGAAGAAATTAAAGAGGCACAAAAGCGTACCCCAGAAACTAGTTTTGCCAGTTTCAAACAAAAGTTTCCCAATAAGGAAAGTTTTGTTAAAGGTTTTCAACCAATTGATGTCTACAACTTACTAAGTGGTTGA", "aa": "MKKLHKILLGLSLPASLAPLSGFISTDADNSQFSLKKSSTEIKGTQSIHLLNLGESLTEEIKEAQKRTPETSFASFKQKFPNKESFVKGFQPIDVYNLLSG", "aa_hexdigest": "d06a012651ff44db3191783cbd8a3a38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 40, "aa_cov": 0.38613861386138615, "hmm_cov": 1.0, "evalue": 2.7794296504869616e-11, "score": 38.68211364746094, "start": 63, "stop": 101 } ], "seq_stats": { "molecular_weight": 11127.642499999985, "isoelectric_point": 9.260899543762207 }, "id": "CNOCLNGIAL_129", "locus": "GCA000143945_00089" }, { "type": "cds", "sequence": "contig_1", "start": 49709, "stop": 50545, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_042", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078", "UniRef:UniRef90_P75072" ], "nt": "GTGCTAAACGTTTTGGGCTCATATGGTGGTGAAGGGTTCTTTCCTGCATTGGGTAAAAACGGGTTGAACCTACCACAACAAATCTTTGAAAACTTCACTGATTTCAAAGTTGTTAGTCATAAAATTCATGACTTCCAAGTAAGTTTGGTAGGCGAACGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAGCTGCTTGTTAACAACCAAAAAGTTACTTTCAACATTACGGTTGATTTGAGAACCAATAATTTCTCTACCCAAGAAACTTTTAACGAGCTGTTCAATAAATGCATTGGCCCCGTTAACTGGCAATTCTTTAGTAGGGTTAAAGTAGATAAATTGCACTACGATCAAACTGACGCTACTCATTTAGCTAACACGTTGTTACAAGACCAGTTCAATGCGTTAAATCTTGATTTAGAAAAGTCCATCTATGACTTGGAACTACCTAGATTAGAAGCAGAGTTCCAACAAAAATACGTTGATCCTTTAATTGAGAAAAAGCAAAGACAAAAAGCAGAGTGGGAAGAAGCGGAACGTATCCGTAAGGAAGAAGAGGAAAAACACCAAAAGGAGCTTGAAGAACAACAAAGAATTCAAGCAGAAAAAGCGAAAAATGACGAACAGTTACGTAAAGCGCAAACTGAATTGAAGAAGGCGCTTGGTGGCATCGACTCCTTTGTTGAATTCTTTACGAATAATGATTTACGTCTTAAACTAGGTTACACCAAAGAAGACAACGTTAGAACCCGCGCTGGACTTTTTAGAGCACTAGAAGTTTCCTTTGGTAATTACCGCGCTTGA", "aa": "MLNVLGSYGGEGFFPALGKNGLNLPQQIFENFTDFKVVSHKIHDFQVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNELFNKCIGPVNWQFFSRVKVDKLHYDQTDATHLANTLLQDQFNALNLDLEKSIYDLELPRLEAEFQQKYVDPLIEKKQRQKAEWEEAERIRKEEEEKHQKELEEQQRIQAEKAKNDEQLRKAQTELKKALGGIDSFVEFFTNNDLRLKLGYTKEDNVRTRAGLFRALEVSFGNYRA", "aa_hexdigest": "d963b090516beb3d84a2dfea7829d06d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 49409, "stop": 50845, "observations": { "mutation": [ 49580, 50543, 50570 ], "stop": [ 49580, 50543, 50570 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75072", "query_cov": 1.0, "subject_cov": 0.41369047619047616, "identity": 0.982, "score": 506.0, "evalue": 9.14e-175, "gene_start": 1, "gene_end": 278, "reference_start": 145, "reference_end": 422, "reference_sequence": "MKKLHKILLGLSLPASLAPLSGFISTDADNSQFSLKKSSTEIKGTQSIHLLNLGESLTEEIKEAQKRTPETSFASFKQKFPNKESFVKGFQPIDVYNLLSGWKDAISSFLDKVVELQKKIEEANKIFNTNIGNQIDLPEDENPNVLNVLGSYGGEGFFPTLGKNGLNLPQQIFENFTDFKVVSHKIHDFQVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNELFNKCIGPVNWQFFSRVKVDKLHYDQTDATHLANTLLQDQFNALNLDLEKSIYDLELPRLEAEFQQKYVDPLIEKKQRQKAEWEEAERIRKEEEEKHQKELEEQQRIQAEKAKNDEQLQKPQTELKKALGGIDSFVEFFTNNDLRLKLGYTKEDNVRTRAGLFRALEVSFGNYRAWTFYITLLGWKDTTEKIFKKAKWQDIRDDEKFRKAFGLSPKATEKDVGKVTNPGYGYQGIYIKDSLRDGIAKYSDSTVSEPKNVKVSLPGTVGDNEEGKIWIASHNFRQNHEWGAGEKFKYSAYRFKFDVTVDYDVEVSAKWWTWAFRGSIPGYWRGKFKVTYSFDGVVPSWKYGHIQVRTPQYSFNKQEQKILFVPHAIQKIAAEGSNLDLINPFLKDQKLDEFEHYHPDLTKPLDLVAYLLYAITTRS" }, "paralog": false, "identity": 0.9853556485355649, "score": 904.0, "evalue": 0.0, "description": "Internal stop codon at 49580, 50543, 50570. Nonsense mutation around 49580, 50543, 50570.", "psc": { "uniref90_id": "UniRef90_P75072", "product": "Uncharacterized protein MPN_042", "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75072", "UniRef:UniRef50_P75078" ] }, "pscc": { "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078" ], "product": "Uncharacterized protein MPN_036" } }, "id": "CNOCLNGIAL_130", "locus": "GCA000143945_00090" }, { "type": "cds", "sequence": "contig_1", "start": 51077, "stop": 51295, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "GTGCGTACGCCACAATACAGCTTTAATAAGCAGGAACAAAAGATTCTGTTTGTACCCCATGCTATCCAAAAGATTGCTGCTGAAGGTAGCAACTTAGATTTAATTAATCCATTCTTAAAGGATCAAAAATTAGATGAATTTGAGCACTACCACCCAGATTTAACTAAACCATTAGATTTAGTAGCTTATCTGTTATACGCAATAACCACGAGATCATAG", "aa": "MRTPQYSFNKQEQKILFVPHAIQKIAAEGSNLDLINPFLKDQKLDEFEHYHPDLTKPLDLVAYLLYAITTRS", "aa_hexdigest": "4b877f46287aea38b401af53343dd049", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 27, "aa_cov": 0.375, "hmm_cov": 0.9629629629629629, "evalue": 7.86213653304928e-08, "score": 27.948936462402344, "start": 2, "stop": 28 } ], "seq_stats": { "molecular_weight": 8417.559499999992, "isoelectric_point": 6.245775413513184 }, "id": "CNOCLNGIAL_131", "locus": "GCA000143945_00091" }, { "type": "cds", "sequence": "contig_1", "start": 51619, "stop": 51786, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATTTAAGTGATTTTAGTGAATTACCACGTTGGATTGGTGCCGAATTTTTAGGCACATTCTTTTTAATTCTTTTAGGTAACGGTGCTGGTTCGCAGCTAACACTCAATAAGATGTTTGTTAAAGAATCGAAAGCAAAGTTACTAACTGCTGCTTTTGCTTGA", "aa": "MFNLSDFSELPRWIGAEFLGTFFLILLGNGAGSQLTLNKMFVKESKAKLLTAAFA", "aa_hexdigest": "649f67f9b4957bab2ab9da7fdf44e849", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6056.0573, "isoelectric_point": 8.190466499328615 }, "id": "CNOCLNGIAL_132", "locus": "GCA000143945_00092" }, { "type": "cds", "sequence": "contig_1", "start": 52054, "stop": 52302, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTACCCATTGTACTAACCCAGCAATCTTTAACATTCCTCGCAACTTTGCAACTGAGTTTGTAGCAACTAGCGTTTTAATTGCTAGTTTACTGGTGGCTGGTAGTTTTGGTGCCAACCGATTTGACCAGTCACCACGAGGGGTTGTACCAATGTTAGTGGTTACTGGCTTAATTATGTCTTTTGGTGCCGCTACTGGTACTGCAATTAACCCAGCCCGTGATTTAGGTCCGAGAATTGTTTACTGA", "aa": "MVTHCTNPAIFNIPRNFATEFVATSVLIASLLVAGSFGANRFDQSPRGVVPMLVVTGLIMSFGAATGTAINPARDLGPRIVY", "aa_hexdigest": "f85be29309d75ea99616540dc29d6a91", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8622.974299999996, "isoelectric_point": 9.486087608337403 }, "id": "CNOCLNGIAL_133", "locus": "GCA000143945_00093" }, { "type": "cds", "sequence": "contig_1", "start": 52384, "stop": 52860, "strand": "-", "frame": 3, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "EC:2.7.1.21", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897", "KEGG:K00857", "SO:0001217", "UniRef:UniRef50_P47280", "UniRef:UniRef90_P75070" ], "nt": "GTGAAATCCCGTAACGGTCACAGTGATGAAGCCATTGAAATTAATTCACCTTTAGAAATCTATGACTACCTCACTAAAGATCGTTTTGATGTGGTTGCCATTGATGAAGCCCAATTCTTTTCGAGTGAAATTGTCGAAGTGGTTAAATCACTCAATGATCTCGGCATTAACGTCATTGTTTCTGGTTTAGACACGGACTTCCGCGCTGAACCCTTTGGTAGCATTCCCCAACTGTTAGCGATTGCCGACAAGATTTGTAAGTTAGATGCCGTTTGTAATGTCTGTGGTCAGTTAGCCCAACGCACCCAGCGCATAGTGTCCAAGAGCAACGAAACCGTTTTAATTGGTGATATTGAAGCTTATGAACCACGTTGTAAGTTGCACCACCACGTGCAGGGTAAGCCAGTTACAGTTAAGACCAAGCAGTTTAAAGAACGCTTTAAGGAACTAAACGATAACAGCCACAAGCACACCTAA", "aa": "MKSRNGHSDEAIEINSPLEIYDYLTKDRFDVVAIDEAQFFSSEIVEVVKSLNDLGINVIVSGLDTDFRAEPFGSIPQLLAIADKICKLDAVCNVCGQLAQRTQRIVSKSNETVLIGDIEAYEPRCKLHHHVQGKPVTVKTKQFKERFKELNDNSHKHT", "aa_hexdigest": "a4658b649b4d25227492bb57443cb055", "start_type": "GTG", "rbs_motif": null, "genes": [ "tdk" ], "truncated": "3-prime", "pseudogene": { "start": 52384, "stop": 53058, "observations": { "mutation": [ 52926, 52995 ], "stop": [ 52926, 52995 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75070", "query_cov": 1.0, "subject_cov": 0.7053571428571429, "identity": 0.9940000000000001, "score": 313.0, "evalue": 4.15e-107, "gene_start": 1, "gene_end": 158, "reference_start": 67, "reference_end": 224, "reference_sequence": "MFNYNWATMSFSQVFHQSPRGWIEVICGPMFSGKTEELLRKIKRWKLAKIPVIIFKPKIDTRQQHLVKSRNGHSDEAIEINSPLEIYDYLTKDRFDVVAIDEAQFFSSEIVEVVKSLNDLGINVIVSGLDTDFRAEPFGSIPQLLAIADKICKLDAVCNVCGQLAQRTQRIVSKSNETVLIGDIEAYEPRCKLHHHVQGKPVTVKTKQFKERFKELNDNSHKHT" }, "paralog": false, "identity": 0.9866071428571429, "score": 443.0, "evalue": 9.08e-161, "description": "Internal stop codon at 52926, 52995. Nonsense mutation around 52926, 52995.", "psc": { "uniref90_id": "UniRef90_P75070", "gene": "tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_P47280", "kegg_orthology_id": "K00857", "go_ids": [ "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75070", "EC:2.7.1.21", "UniRef:UniRef50_P47280", "KEGG:K00857", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ] }, "pscc": { "uniref50_id": "UniRef50_P47280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47280" ], "product": "Thymidine kinase" } }, "id": "CNOCLNGIAL_134", "locus": "GCA000143945_00094" }, { "type": "cds", "sequence": "contig_1", "start": 53193, "stop": 53588, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30", "PFAM:PF01409.25", "PFAM:PF13393.11" ], "nt": "TTGGAATACGCTGAAGCCTTTAAGCGCACCAATGCCAACGCCGATATTGTCAAGAAGGAGTTGTATGAATTCCATGACAAATCGAACCGGCTGTTAGCCCTGCGTCCCGAGGCTACTGCTGGGATAGTGCGCTTGGTCTGTGAGAACAAACTACTCCAACCACAAAACTACCCGTTAAGGTTGTTTACCATTGGCACCATGTACCGGTATGAACGCCCCCAAAGTAACCGTTACCGCGAGCACTACCAGTTTAGTTGTGAAGTGATAGGTGACACTAATCCAACCGTGTTACTCGACACTTTACTGTTGGGTCATGCCATTATCCAGCAATTGGGAATTGAAGGAGTGATCCTCAAACTTAACAACTTAGGTAACAGTGCTACCATTCAACAATGA", "aa": "MEYAEAFKRTNANADIVKKELYEFHDKSNRLLALRPEATAGIVRLVCENKLLQPQNYPLRLFTIGTMYRYERPQSNRYREHYQFSCEVIGDTNPTVLLDTLLLGHAIIQQLGIEGVILKLNNLGNSATIQQ", "aa_hexdigest": "250c2e5a0a23376fea71c4fd1bd25ebd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 105, "aa_cov": 0.7557251908396947, "hmm_cov": 0.9904761904761905, "evalue": 1.1382675138374746e-12, "score": 43.79168701171875, "start": 22, "stop": 120 }, { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 74, "aa_cov": 0.549618320610687, "hmm_cov": 0.9324324324324325, "evalue": 1.292242307203884e-05, "score": 20.253562927246094, "start": 21, "stop": 92 }, { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 123, "aa_cov": 0.9389312977099237, "hmm_cov": 0.9512195121951219, "evalue": 1.939033830905967e-19, "score": 65.43927001953125, "start": 2, "stop": 124 } ], "seq_stats": { "molecular_weight": 15053.090699999984, "isoelectric_point": 7.7830907821655275 }, "id": "CNOCLNGIAL_135", "locus": "GCA000143945_00095" }, { "type": "cds", "sequence": "contig_1", "start": 53802, "stop": 54101, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "GTGCCGTTTGAGTGGAATCCAACCCTAGTCCGGGGCTTGGACTACTATACTGGGGTAGTGTTTGAATTTGTTAAGGATGACACCACGGTACTAGCTGGTGGGGTGTATGATAACCTAGTGGAAGAGTTAGGTGGTACACCTACTAAAGCGCTGGGTTTTGCTTGTGGCATTGAACGCTCAATTAATTGTTTAAGTGCGGTTAAAAAGCAAGCAATTTTGGCCAATCAACCCCCACGATTACTGGTTATAGGTCTCACCGAAGCAGCGTTGGAAAAGCTGTTACAACTTTCATTGGGTTGA", "aa": "MPFEWNPTLVRGLDYYTGVVFEFVKDDTTVLAGGVYDNLVEELGGTPTKALGFACGIERSINCLSAVKKQAILANQPPRLLVIGLTEAALEKLLQLSLG", "aa_hexdigest": "200267869656c59ceb5b281052b89fee", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 57, "aa_cov": 0.5555555555555556, "hmm_cov": 0.9824561403508771, "evalue": 7.556997084759354e-13, "score": 43.79265213012695, "start": 4, "stop": 58 } ], "seq_stats": { "molecular_weight": 10683.288399999989, "isoelectric_point": 4.761254692077636 }, "id": "CNOCLNGIAL_136", "locus": "GCA000143945_00096" }, { "type": "cds", "sequence": "contig_1", "start": 54117, "stop": 54275, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "GTGACTATTTATCCTAAAGTTATTAGAATTATCAATGGCATCAGAGCCGCGCAACGGTTAGGTTACCGCTTTTTAGGGGTCATTGGTGGTAATAATTTAGAACAGCAAACCATTACCGTTAAAGACCTGGCTACTGAACAGCAAACAACATACACTTGA", "aa": "MTIYPKVIRIINGIRAAQRLGYRFLGVIGGNNLEQQTITVKDLATEQQTTYT", "aa_hexdigest": "edd2793ff4e7153f8d48217d15fe296e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 39, "aa_cov": 0.75, "hmm_cov": 1.0, "evalue": 2.9003144895557537e-07, "score": 26.083879470825195, "start": 12, "stop": 50 } ], "seq_stats": { "molecular_weight": 5868.7206000000015, "isoelectric_point": 9.815069770812986 }, "id": "CNOCLNGIAL_137", "locus": "GCA000143945_00097" }, { "type": "cds", "sequence": "contig_1", "start": 54277, "stop": 54720, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAATTTAGACAACGCCAAGTGCTTTAAGCAACGGGTTTTTATTGGCAATTTAACTTCCGAGCACCTCAATAAAACCGTTACTATTGCCGGATGGGTCAAACGGATTAAGAAGTTAGGTGAACTTAACTTTGTTATTGTCGGGGACAAGACTAATACTATTCAGGTTACCTGTAAAAATAAGGAACAGGTCAAGTACTTAACCAGAGAAGACTTGGTGATTGTCAAGGGCAAACTGAAACGCTTAGACAGTGTTCGGTTTGAAATTACCAATCCTACTATTACCTTATTTGCCAAGTCCAAAACACCCCCTCTTATCATTGAGGACAAAACTGATGCTTTAGAAGAAGTGCGTTTGCGTTACCGCTACTTAGACTTACGCCGTCCGGTGATGCAAAAGCGCTTGGCGTTACGCCATAAGGTAACTTTAGCCGTCCGGAACTGA", "aa": "MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKNKEQVKYLTREDLVIVKGKLKRLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLRYRYLDLRRPVMQKRLALRHKVTLAVRN", "aa_hexdigest": "4494097b7192b7952a9407b945689d6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 74, "aa_cov": 0.47619047619047616, "hmm_cov": 1.0, "evalue": 1.7986615518680377e-15, "score": 52.1613655090332, "start": 27, "stop": 96 } ], "seq_stats": { "molecular_weight": 17120.06379999999, "isoelectric_point": 10.203362846374514 }, "id": "CNOCLNGIAL_138", "locus": "GCA000143945_00098" }, { "type": "cds", "sequence": "contig_1", "start": 54730, "stop": 55407, "strand": "+", "frame": 1, "gene": "aspS", "product": "aspartate--tRNA ligase", "db_xrefs": [ "COG:COG0173", "COG:J", "EC:6.1.1.-", "EC:6.1.1.12", "GO:0003676", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422", "SO:0001217", "UniRef:UniRef50_P75068", "UniRef:UniRef90_P75068" ], "nt": "ATGGGGTTTATTGAAGTAGAAACACCTACGCTCACGAAGTCCACACCGGAGGGTGCTAGGGACTTTTTGGTACCGGCCAGAATTAGGGAACATAGCTTTTACGCTTTGCCCCAAAGTCCACAAATTTATAAACAGTTATTAATGGTTGGTGGTACGGAAAAGTACTTCCAAATTGCCCATGTTTACCGCGATGAAGACAGCCGCAAAGACCGTCAACCTGAACACACCCAAATTGACTTAGAGGTGGCCTTTTACACCAAGGAAATGGTGATGGATTTAATCCAAAGGTTATTTGTCGATGTCTTTCGCCAAGTGTTAAACATTAAGCTCAAAAAACCGTTTCCAGTGTTAAAGTTTGCTGAAGCCTTTAACCGTTTTGGCAGTGACAAACCGGATTTACGTTATGGTTTCGAACTGGAAGACTGCACTGATTTATTCCAAGACAGTCCGAACCAATTCACTAACTTAATTAATGCTGGCGGCATAGTCGGTGGGATCCAATTACCGAATCTTTATTTAGACGAAGTTTCTTTTAAAGCATTGAGGAAACTAGCTAAGGACAACGGGGTTTCCTTAGAGTTTTACTCTGATAAAGCTAGTTCCCTAAAACAACCCCTGGATTTACCGTTAGCAGGCACCATCTTACTGGTAGCACACAAAAGTAAAACGCAAGCTTGA", "aa": "MGFIEVETPTLTKSTPEGARDFLVPARIREHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEMVMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSPNQFTNLINAGGIVGGIQLPNLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPLAGTILLVAHKSKTQA", "aa_hexdigest": "3eb50f3ccdf669972a263758bb687614", "start_type": "ATG", "rbs_motif": null, "genes": [ "aspS" ], "truncated": "both", "pseudogene": { "start": 54430, "stop": 55710, "observations": { "mutation": [ 54718, 55405, 55483 ], "stop": [ 54718, 55405, 55483 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75068", "query_cov": 1.0, "subject_cov": 0.40394973070017953, "identity": 1.0, "score": 448.0, "evalue": 2.88e-154, "gene_start": 1, "gene_end": 225, "reference_start": 152, "reference_end": 376, "reference_sequence": "MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKNKEQVKYLTREDLVIVKGKLKRLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLRYRYLDLRRPVMQKRLALRHKVTLAVRNWLDQMGFIEVETPTLTKSTPEGARDFLVPARIREHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEMVMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSPNQFTNLINAGGIVGGIQLPNLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPLAGTILLVAHKSKTQAWTALGAIRNELKYHLNLVKPNQYSFCWIVDFPLYEFDEKEQKWVSAHNMFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGVDPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVDIKDITTIWSKHPVK" }, "paralog": false, "identity": 0.9929577464788732, "score": 839.0, "evalue": 8.04e-311, "description": "Internal stop codon at 54718, 55405, 55483. Nonsense mutation around 54718, 55405, 55483.", "psc": { "uniref90_id": "UniRef90_P75068", "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.-", "6.1.1.12" ], "uniref50_id": "UniRef50_P75068", "cog_id": "COG0173", "cog_category": "J", "go_ids": [ "GO:0003676", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75068", "EC:6.1.1.-", "EC:6.1.1.12", "UniRef:UniRef50_P75068", "COG:COG0173", "COG:J", "GO:0003676", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ] }, "pscc": { "uniref50_id": "UniRef50_P75068", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75068" ], "product": "Aspartate--tRNA ligase" } }, "id": "CNOCLNGIAL_139", "locus": "GCA000143945_00099" }, { "type": "cds", "sequence": "contig_1", "start": 55549, "stop": 55950, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGTTCTCCAATCCCCAACCGCAGTGGTTGGTTAACTTTGAAAACCACAAGGCTGAGGCGTTGAGTGAACAGTACGACCTAGTTTTAAATGGGTTTGAACTGGGTAGTGGTTCAATTCGGATCCATGACCCAGAAGTACAAACCCGTTTAATGCAATCTTTAGGGGTGGATCCCCAACAGTTTGGCTTTGTGATGGAGGCTTACCAGTACGGTGCTCCTGTACACGCTGGCATGGGTTTGGGATTAGACCGCTTAATGATGATCATTAACAATGTGGACAACATTAGGGAAGTGATGGCCTTTCCGAAAAATGCCCAAGGGATAGAGATGCACACTAATGCCCCTGATCAGGTTGACATTAAAGACATAACAACAATATGGTCCAAACACCCAGTGAAATAA", "aa": "MFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGVDPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVDIKDITTIWSKHPVK", "aa_hexdigest": "23ff0b70d9f50eeac3df90f955241f03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 93, "aa_cov": 0.6691729323308271, "hmm_cov": 1.0, "evalue": 3.487737121343399e-33, "score": 110.4085464477539, "start": 16, "stop": 104 } ], "seq_stats": { "molecular_weight": 15009.934499999992, "isoelectric_point": 5.019872093200683 }, "id": "CNOCLNGIAL_140", "locus": "GCA000143945_00100" }, { "type": "cds", "sequence": "contig_1", "start": 55926, "stop": 56087, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCCAAACACCCAGTGAAATAAATACCCACCTTAAACACCTCTTGGCCTGTGATGCTTATAAGCTATCACACCGCTTAATGTATCCTAATGACACGACCAACTTATACAGTTGTTTAACAGCCCGGGGTGGTCGTGGTGGTTTTCCAAATTTTGTGTGA", "aa": "MVQTPSEINTHLKHLLACDAYKLSHRLMYPNDTTNLYSCLTARGGRGGFPNFV", "aa_hexdigest": "a54b422c165d3831a2a41c843d2a73cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5953.767000000001, "isoelectric_point": 8.770359611511232 }, "id": "CNOCLNGIAL_141", "locus": "GCA000143945_00101" }, { "type": "cds", "sequence": "contig_1", "start": 56121, "stop": 56432, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGGTAACTTTTGTGATAGTGTGTTAGCAGTCCAAAACGATCCTGGGTTGGCCCAAGCGCTCACTGACAAGGTGACCACTGTTTTTGGTGATCCCCAGTTTGGGTTGGAGTTTACCCAGCACATCTGTTATTTAGCTAACTTTTTAAAACAACACCACCAGTTACCCTTAACGGTAAAGATTCACCAGAGTTCTGAGGGTTTAGCCTTCCGTACACCCTTAGTAACTATTACAGGTTCGGACCAAATGGTTCCCGAGTTAGTTTGGTTAGTCAACTATTTTGAAACCGTATTATTGGAAAACATCTGA", "aa": "MFGNFCDSVLAVQNDPGLAQALTDKVTTVFGDPQFGLEFTQHICYLANFLKQHHQLPLTVKIHQSSEGLAFRTPLVTITGSDQMVPELVWLVNYFETVLLENI", "aa_hexdigest": "e8f9f37f8bfa9a4aa3e2a29b4e605e02", "start_type": "GTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11552.11759999999, "isoelectric_point": 4.8213903427124025 }, "id": "CNOCLNGIAL_142", "locus": "GCA000143945_00102" }, { "type": "cds", "sequence": "contig_1", "start": 56433, "stop": 57188, "strand": "+", "frame": 3, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:2.4.2.12", "EC:6.3.4.21", "GO:0004514", "GO:0009435", "GO:0047280", "SO:0001217", "UniRef:UniRef50_P75067", "UniRef:UniRef90_P75067" ], "nt": "TTGTACCAAACTACTTTAACTGTCGCCCAAAGCTTAAAGCTGCTGTTGGAACGCTATGCTAACGAAACCGCGGATAATACTGAATTTACCCACTTTCAGTGTCATGATTTTAGTATGCGTGGTATGAGTAGTCTTCAAAGTGCTTTATATGTAGCTAATGCTCACCTGCAGTACTTTAGTGGCAGTGACACCATTTTAGGCGGTGTAGCTGCTAAATCGATCTTAGCTAGTGAACACTCGGTGATGTGTGCTGATGGTCAAGAGGGTGAATTGAATACCTTTAAACGTTTATTGGAGCAGTTTCCTAACAAAAATCTGTCCTTAGTGATTGATTCTTATGACATGTGGCATGTTCTAGATAACATCTTACCCCAACTCAAGGACTTAGTTTTACAACGACAGGAGAAGCTGTACCTCCGACCTGACTCGGGCAACTTTGAAACCCTTATTTGTCAGGGCAAACGGTTTAACCCCGAGGACAAAACTACCTGGGGAGTAATTGATTACTTAGATTACCACTTTGGTTCCACTGTTAACCAAAAAGGTTATAAGGTTTTGAACCAAAAGTTGGGTATTGTTTATGGCGATGGCATTACCTATGAGCGGATTGAGTACATTTTGGAACAGCTCAAACAACGTGGGTTTTGTTCTTCCAACATTGTCTTTGGGGTTGGTAGTACAACTTACCAAAACCTTAACCGTGATACGTTGGGATTTGTGTATAAGTTGACAGCCATCAAAAAGGGTAATACCTGA", "aa": "MYQTTLTVAQSLKLLLERYANETADNTEFTHFQCHDFSMRGMSSLQSALYVANAHLQYFSGSDTILGGVAAKSILASEHSVMCADGQEGELNTFKRLLEQFPNKNLSLVIDSYDMWHVLDNILPQLKDLVLQRQEKLYLRPDSGNFETLICQGKRFNPEDKTTWGVIDYLDYHFGSTVNQKGYKVLNQKLGIVYGDGITYERIEYILEQLKQRGFCSSNIVFGVGSTTYQNLNRDTLGFVYKLTAIKKGNT", "aa_hexdigest": "4cdaf15c782691db99387183e9b5587d", "start_type": "TTG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 56133, "stop": 57281, "observations": { "mutation": [ 56430, 57186 ], "stop": [ 56430, 57186 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75067", "query_cov": 1.0, "subject_cov": 0.5565410199556541, "identity": 0.996, "score": 507.0, "evalue": 1.38e-178, "gene_start": 1, "gene_end": 251, "reference_start": 170, "reference_end": 420, "reference_sequence": "MVQTPSEINTHLKHLLACDAYKLSHRLMYPNDTTNLYSCLTARGGRGGFPNFVWNHEFAKKIILEVFGNFCDSVLAVQNDPGLAQALTDKVTTVFGDPQFGLEFTQHICYLANFLKQHHQLPLTVKIHQSSEGLAFRTPLVTITGSDQMVPELVWLVNYFETVLLENIWLYQTTLTVAQSLKLLLERYANETADNTEFTHFQCHDFSMRGMSSLQSALYVANAHLQYFSGSDTILGGVAAKSILASEHSVMCADGQEGELNTFKRLLEQFPNKNLSLVIDSYDMWHVLDNILPQLKDLVLQRQEKLYLRPDSGNFETLICQGKRFNPEDKTTWGVIDYLDYHFGSTVNQKGYKVLNQKLGIVYGDGITYERIEYILEQLKQRGFCSSNIVFGVGSTTYQNLNRDTLGFVYKLTAIKKGNTWHDVTKSPITDPTKQSIGGRFDNPNLIQVYG" }, "paralog": false, "identity": 0.9947643979057592, "score": 768.0, "evalue": 4.61e-284, "description": "Internal stop codon at 56430, 57186. Nonsense mutation around 56430, 57186.", "psc": { "uniref90_id": "UniRef90_P75067", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "2.4.2.12", "6.3.4.21" ], "uniref50_id": "UniRef50_P75067", "cog_id": "COG1488", "cog_category": "H", "go_ids": [ "GO:0004514", "GO:0009435", "GO:0047280" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75067", "EC:2.4.2.12", "EC:6.3.4.21", "UniRef:UniRef50_P75067", "COG:COG1488", "COG:H", "GO:0004514", "GO:0009435", "GO:0047280" ] }, "pscc": { "uniref50_id": "UniRef50_P75067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75067" ], "product": "Nicotinamide phosphoribosyltransferase" } }, "id": "CNOCLNGIAL_143", "locus": "GCA000143945_00103" }, { "type": "cds", "sequence": "contig_1", "start": 57586, "stop": 57741, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCCGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGCCTTTGCTAAACAAAAGGATTGA", "aa": "MSQHGSSTDPSFVALRNNSSLSRDGKSRLLEFGNDLGFSKEETDAFAKQKD", "aa_hexdigest": "864a58654bf19d804a07fed2c7e58588", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5607.013900000001, "isoelectric_point": 5.658912849426269 }, "id": "CNOCLNGIAL_144", "locus": "GCA000143945_00104" }, { "type": "cds", "sequence": "contig_1", "start": 57871, "stop": 58296, "strand": "+", "frame": 1, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C", "UniRef:UniRef90_UPI000A7C0B9C" ], "nt": "ATGAACGAGTTGCAAACCAAAGTCAATGAGGCTAATAATATTTTCCCAGTTGAAGCCTTCAAGGTACCTAAGGTTCCAGAAAAACTGTTTGGTTTTGTCAACCAAGGCTTCTTTCCTAAACTAAACCCTAAGGGCTTAAACATTGCTGATAACGTGGCTTCCTTATTTGAACAATACAGCTTAAAGCAAGCAAGTCTTAAGGACTTTGACATATTATTGGAAAAGAAAAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTAACTTTGAAACCACTTATGTTGGTACTGGTGGGGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACTACTAACTTTAGTAGCTTAGAAGAACTCCAAGCTAGCTTTAGTAAAACAGGCGATAACCTTACTGCCCAATTGTTCTGA", "aa": "MNELQTKVNEANNIFPVEAFKVPKVPEKLFGFVNQGFFPKLNPKGLNIADNVASLFEQYSLKQASLKDFDILLEKKNDIVLEHKVRYNFALQFNFETTYVGTGGEINLQFALQASTTNFSSLEELQASFSKTGDNLTAQLF", "aa_hexdigest": "5ba7808a9df5895b500f696b61ed0ffa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 57586, "stop": 58326, "observations": { "mutation": [ 57739, 58294 ], "stop": [ 57739, 58294 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A7C0B9C", "query_cov": 1.0, "subject_cov": 0.5145985401459854, "identity": 0.9790000000000001, "score": 273.0, "evalue": 5.33e-91, "gene_start": 1, "gene_end": 141, "reference_start": 96, "reference_end": 236, "reference_sequence": "MSQHGSSTDPSFVVLRNNSSLSRDGKSRLLEFGNDLGFSKEETDAFAKQKDWNQNFVKFQKQFENKLLDPKNFSLTDVYNLFSGFQQSVTATVQLMNELQTKVNEANNIFPVEAFKVPKVPEKLFGFVNQGFFPKLNPKGLNIADNVASLFEQYSLKQASLKDFDILLEKKNDIVLEHKVRYNFALQFHFETTYIGSGGEINLQFALQASTTNFSSLEELQASFSKTGDNLTAQLFWKPTVTKLVSTLKPLMLVLVGKLTYSLHCKLPLLTLVA" }, "paralog": false, "identity": 0.9715447154471545, "score": 471.0, "evalue": 3.19e-171, "description": "Internal stop codon at 57739, 58294. Nonsense mutation around 57739, 58294.", "psc": { "uniref90_id": "UniRef90_UPI000A7C0B9C", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000A7C0B9C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A7C0B9C", "UniRef:UniRef50_UPI000A7C0B9C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A7C0B9C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C" ], "product": "DUF240 domain-containing protein" } }, "id": "CNOCLNGIAL_145", "locus": "GCA000143945_00105" }, { "type": "cds", "sequence": "contig_1", "start": 58495, "stop": 58614, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCATTAAGAAAGCTGAAGAAGAGAAAATTAAAAAAGAACTTGAAGAGCAAAAGAAGCGTCAAGAAGAATTAGCAAAACAACAACGAGACAAAGAGGCATTACAAAAGTCACTTTGA", "aa": "MAIKKAEEEKIKKELEEQKKRQEELAKQQRDKEALQKSL", "aa_hexdigest": "39fbb92d0982d849d79b07c8a791bc5e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4711.3996, "isoelectric_point": 9.04905643463135 }, "id": "CNOCLNGIAL_146", "locus": "GCA000143945_00106" }, { "type": "cds", "sequence": "contig_1", "start": 58732, "stop": 59001, "strand": "+", "frame": 1, "gene": null, "product": "DUF237 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AE7A69C", "UniRef:UniRef90_UPI000AE7A69C" ], "nt": "TTGTTAATTGCCGGTTTTAGAAGTGCAATTCAGACTTATTACAAAGATGGCAAAGCAGATCAAAGCCAAAACGCTAAAATTGCTTTTGGCGAAAAGGGTATTCAATTCCCTAAATCTGGCCCAGGACTTGACGGTATTTTCATGTCAGATTTCCTTCGTGGTAACCTTACCGGCAACGCTCACTTTGATTTAAAACTTAAAAAAGTTGAAGTTAAAAACACACAAGGAAAAGACGCACAAGGCAATGATAAAAAAGCCTCCATTAATTGA", "aa": "MLIAGFRSAIQTYYKDGKADQSQNAKIAFGEKGIQFPKSGPGLDGIFMSDFLRGNLTGNAHFDLKLKKVEVKNTQGKDAQGNDKKASIN", "aa_hexdigest": "4fee2ed8116e4876e8dbdb85b3183d26", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 58732, "stop": 59304, "observations": { "mutation": [ 58999, 59149 ], "stop": [ 58999, 59149 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AE7A69C", "query_cov": 1.0, "subject_cov": 0.43842364532019706, "identity": 1.0, "score": 178.0, "evalue": 3.19e-55, "gene_start": 1, "gene_end": 89, "reference_start": 1, "reference_end": 89, "reference_sequence": "MLIAGFRSAIQTYYKDGKADQSQNAKIAFGEKGIQFPKSGPGLDGIFMSDFLRGNLTGNAHFDLKLKKVEVKNTQGKDAQGNDKKASINWQAKQNNFPFRQVNPWDFSFEVELKYEGSYGLYPGARFLNLFGSLGIPNDWKGEMSVKFVLDGKTPQWIADKPDYPGSLFKFEKNQLKFTPHVKEHVHVENKQFMEKLKESKLA" }, "paralog": false, "identity": 0.9842105263157894, "score": 387.0, "evalue": 7.3e-140, "description": "Internal stop codon at 58999, 59149. Nonsense mutation around 58999, 59149.", "psc": { "uniref90_id": "UniRef90_UPI000AE7A69C", "product": "DUF237 domain-containing protein", "uniref50_id": "UniRef50_UPI000AE7A69C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AE7A69C", "UniRef:UniRef50_UPI000AE7A69C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AE7A69C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AE7A69C" ], "product": "DUF237 domain-containing protein" } }, "id": "CNOCLNGIAL_147", "locus": "GCA000143945_00107" }, { "type": "cds", "sequence": "contig_1", "start": 59161, "stop": 59343, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAGCGTTAAGTTTGTGCTCGATGGTAAGACACCTCAATGGATTGCTGATAAACCAGATTACCCAGGTTCCTTGTTTAAATTTGAAAAGAACCAGTTAAAGTTCACTCCCCACGTTAAGGAGCATGTACATGTTGAAAACAAACAATTTATGGAAAAGTTGAAAGAGTCAAAACTTGCATAA", "aa": "MSVKFVLDGKTPQWIADKPDYPGSLFKFEKNQLKFTPHVKEHVHVENKQFMEKLKESKLA", "aa_hexdigest": "b182c67bca38873455c7b539de984f60", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 48, "aa_cov": 0.7833333333333333, "hmm_cov": 1.0, "evalue": 4.974540148598708e-18, "score": 60.979270935058594, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 7073.1571, "isoelectric_point": 9.302739524841307 }, "id": "CNOCLNGIAL_148", "locus": "GCA000143945_00108" }, { "type": "cds", "sequence": "contig_1", "start": 59605, "stop": 60393, "strand": "+", "frame": 1, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C" ], "nt": "ATGCGCTTTCGGTATAAGGCGTTTCTACTAACTTTATTTGCGTCAACCACAACGCTTACTGGATTCATAATACCTACTTTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCTGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGTGTTAGCGAAACAGAAAAACTGGGAGCAGAATTACACTAACTTTAAAAAGCAGTTTGAACACAAGCTTTTAGATCCGAAGAGCTTTAGCTTAACTGATGTGTATAACCTCTTTAGTGGTTTTCAACAAAGTGTAGCTGACACGGTTAAGCTAATGAATGAATTGCAAACTCAGGTAAATAAAGCTAACGACATTTACCCAGTGGAAAGCTTTCAAGTGCCTAAGGTACCACAAAAGCTATTTGGATTTGTCGACCAGGGCTTCTTTCCGAAGTTAAACCCGAAGGGCTTGAACATTGCTGATAACGTGGCTTACTTGTTTGAGAAGTACAGCCTAAAACAAGCAACCCTTAAAGACTTTGACATCGTTTTGGAAAAGAAGAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTCACTTTGAAACTACATACATAGGTAGCGGTGGCGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACCACTAACTTTAGTAGTTTAGAGGAATTGCAAGCTAGCTTTAGTAAGGTAGGTAATAATTTAACTGCTCAATTGTTCTGA", "aa": "MRFRYKAFLLTLFASTTTLTGFIIPTLSQHGSSTDPSFVALRNNSSLSCDGKSRLLEFGNDLGFSKEETDVLAKQKNWEQNYTNFKKQFEHKLLDPKSFSLTDVYNLFSGFQQSVADTVKLMNELQTQVNKANDIYPVESFQVPKVPQKLFGFVDQGFFPKLNPKGLNIADNVAYLFEKYSLKQATLKDFDIVLEKKNDIVLEHKVRYNFALQFHFETTYIGSGGEINLQFALQASTTNFSSLEELQASFSKVGNNLTAQLF", "aa_hexdigest": "e14ddca2b71544650656a86e88412dcd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A7C0B9C", "query_cov": 0.9007633587786259, "subject_cov": 0.8613138686131386, "identity": 0.873, "score": 409.0, "evalue": 2.66e-142, "valid": false, "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000A7C0B9C" }, "pscc": { "uniref50_id": "UniRef50_UPI000A7C0B9C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C" ], "product": "DUF240 domain-containing protein" }, "genes": [], "id": "CNOCLNGIAL_149", "locus": "GCA000143945_00109" }, { "type": "cds", "sequence": "contig_1", "start": 60820, "stop": 61074, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTCAATTATTAACTGCTGGAATGCGTGAAGGTATTAAGGAGTATTACAAACATAATAAACCTGACCAAAGCGCTAATGCTAAAAAAGCCTTTGGCCAAAATGGTCTTGCATTTCCGCGAACTGGCTTTGATGGTATCTACATGTCTGACTGGCTTCGTGGTGAATTGAGGAATAAGGGTAACATCAATCTCCATCTCAAACAAAACGAAACCACCGTTAAAAAGATTAGGGATGACATTAGTATCGAATGA", "aa": "MFQLLTAGMREGIKEYYKHNKPDQSANAKKAFGQNGLAFPRTGFDGIYMSDWLRGELRNKGNINLHLKQNETTVKKIRDDISIE", "aa_hexdigest": "52118a002728cc58b72aebcc472f0521", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9658.885399999994, "isoelectric_point": 9.520384788513184 }, "id": "CNOCLNGIAL_150", "locus": "GCA000143945_00110" }, { "type": "cds", "sequence": "contig_1", "start": 62104, "stop": 62502, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02782.22" ], "nt": "ATGTACTCGGCAGCTGAAAGTGACTTTTATGCTAAGCTAGCACAAAAGGAAGAACAGGAAGTGGTGTTTGTCCCAGCCTTCACTGGTTTAGGCGCACCTTACTGGGATGCTTCAGCAAGAGGCGCCATCTTTGGGATAGAAGCTAATACCAAAAGGGAACATTTAGTAAAAGCTACCTTGGAAGCAATTGCCTTTCAAGCTAATGACCTGATTAAGGCAATGGCTAGTGATTTAAACAGTTCTATTAAAAAAATTAAGGCTGATGGCGGTGCTTGCAATTCCAACTATTTAATGCAATTTCAAGCTGATATTGCTAACTTAGAGGTGATTATTCCTAAGAATGTGGAAACCACTGCCATGGGTGCGGCCTTCCTAGCTGGTTTAGCAGTTAATTATTGA", "aa": "MYSAAESDFYAKLAQKEEQEVVFVPAFTGLGAPYWDASARGAIFGIEANTKREHLVKATLEAIAFQANDLIKAMASDLNSSIKKIKADGGACNSNYLMQFQADIANLEVIIPKNVETTAMGAAFLAGLAVNY", "aa_hexdigest": "38e76e5f49cf0e126bc0b130ac86a101", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02782.22", "name": "FGGY_C", "length": 119, "aa_cov": 0.8939393939393939, "hmm_cov": 1.0, "evalue": 3.463798113814519e-34, "score": 113.78630828857422, "start": 12, "stop": 129 } ], "seq_stats": { "molecular_weight": 14170.988799999977, "isoelectric_point": 4.910002326965332 }, "id": "CNOCLNGIAL_151", "locus": "GCA000143945_00111" }, { "type": "cds", "sequence": "contig_1", "start": 62524, "stop": 62766, "strand": "-", "frame": 3, "gene": null, "product": "FGGY-family carbohydrate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030F9964F" ], "nt": "ATGGTACCTATTCGTGGGGTTGCCGGTGACCAACAAGCAGCCTTGTTTGGTCAGCTCTGCGTGGAACCAGCGATGGTTAAAAACACCTATGGTACAGGTTGTTTTATGTTGATGAACATCGGTAACGAACTGAAATACTCCCAACATAACTTATTAACAACTGTAGCTTGGCAGTTAGAAAACCAAAAACCGGTGTATGCCTTAGAAGGTAGTGTTTTTGTGGCTGGCGCAGCCTTAAAGTGA", "aa": "MVPIRGVAGDQQAALFGQLCVEPAMVKNTYGTGCFMLMNIGNELKYSQHNLLTTVAWQLENQKPVYALEGSVFVAGAALK", "aa_hexdigest": "859321ebd9901156384390060af99b60", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030F9964F", "query_cov": 0.9625, "subject_cov": 0.9625, "identity": 0.5579999999999999, "score": 90.9, "evalue": 1.94e-22, "valid": false, "product": "FGGY-family carbohydrate kinase", "uniref50_id": "UniRef50_UPI0030F9964F" }, "pscc": { "uniref50_id": "UniRef50_UPI0030F9964F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030F9964F" ], "product": "FGGY-family carbohydrate kinase" }, "genes": [], "id": "CNOCLNGIAL_152", "locus": "GCA000143945_00112" }, { "type": "cds", "sequence": "contig_1", "start": 63052, "stop": 63201, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00370.27" ], "nt": "TTGCCCGTTTACAATGCAATAGTATGGCAAGATCAACGCACTGCTAGTTTGTGTGATAAGTTAAACCAAGACACCAAGATAAAGGAGTTTGTCAAGAAACACACTGGTTTACCAATTAACCCTTATTTTAGTGCCACTAAGATTGCTTGA", "aa": "MPVYNAIVWQDQRTASLCDKLNQDTKIKEFVKKHTGLPINPYFSATKIA", "aa_hexdigest": "b0da8fd54b555938f2786369a3176fd6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00370.27", "name": "FGGY_N", "length": 47, "aa_cov": 0.9591836734693877, "hmm_cov": 0.9787234042553191, "evalue": 8.979459069122027e-13, "score": 43.81355667114258, "start": 2, "stop": 48 } ], "seq_stats": { "molecular_weight": 5623.4634000000015, "isoelectric_point": 9.302159309387207 }, "id": "CNOCLNGIAL_153", "locus": "GCA000143945_00113" }, { "type": "cds", "sequence": "contig_1", "start": 63217, "stop": 63306, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGTGCTAAAAATAAGGCACAAATTAAATCAAACAACATTGCTGCTGTAGGGATTACCAACCAACGGGAAACCATTGTGTTATGA", "aa": "MQSAKNKAQIKSNNIAAVGITNQRETIVL", "aa_hexdigest": "e00dcb1327047a07f6c13396c7acb3ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3141.6012, "isoelectric_point": 10.288976860046386 }, "id": "CNOCLNGIAL_154", "locus": "GCA000143945_00114" }, { "type": "cds", "sequence": "contig_1", "start": 63325, "stop": 63489, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00370.27" ], "nt": "ATGGATCTAAAACAACAATACATTCTTGCTATAGATGAAGGAACCAGTTCTTGCCGCACGATTGTGTTTGACAAGGATTTAAACCAAGTAGCGATTGCCCAAAACGAATTTAACCAGTTCTTTCCTAAGAGTGGTTGGGTCGAACAATATCCCTTAGAAATTTGA", "aa": "MDLKQQYILAIDEGTSSCRTIVFDKDLNQVAIAQNEFNQFFPKSGWVEQYPLEI", "aa_hexdigest": "17ff5528ec5f4b3501aa057311e5cf88", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920FAT8", "query_cov": 0.9074074074074074, "subject_cov": 0.8909090909090909, "identity": 0.612, "score": 67.8, "evalue": 4.98e-14, "valid": false, "product": "Carbohydrate kinase FGGY N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A920FAT8" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00370.27", "name": "FGGY_N", "length": 48, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 5.4619397238272324e-11, "score": 37.97089385986328, "start": 7, "stop": 54 } ], "seq_stats": { "molecular_weight": 6283.0147, "isoelectric_point": 4.285114479064942 }, "id": "CNOCLNGIAL_155", "locus": "GCA000143945_00115" }, { "type": "cds", "sequence": "contig_1", "start": 63510, "stop": 63932, "strand": "-", "frame": 1, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "nt": "GTGGTGGTAACTAATCAGGGCGAACTCCACTTAAACTCAATGGTTTTCATGGTACCAACAATCCATGGTAAAGGGGTAATTGTGTCACCCATGTTAGATGGCAACTTCTTAGTCGGACCCACCGCTTTAGATGGGGTAGACAAGGAAGCTACTCGTTACATTACCAAAGATGCTCCATGTATGTTGACTAAGATTGGCAAACATATGGTGCCAAGTCTAAACATTAATAATGCCTTAATAAGCTTTGCTGGTTCGCGTCCAATTGATAAAGCCACTAATGACTTTATTATTAGAATAGCTCACAATGACCCTGACTTTGTCATTTTAGGGGGTATGAAATCCCCAGGGCTAACTGCAGCACCAGCCATTGCCCGGGAAGCTGTCAGACTTTTAAATTGGAAATTAACTAAAAAGCCGAACTGA", "aa": "MVVTNQGELHLNSMVFMVPTIHGKGVIVSPMLDGNFLVGPTALDGVDKEATRYITKDAPCMLTKIGKHMVPSLNINNALISFAGSRPIDKATNDFIIRIAHNDPDFVILGGMKSPGLTAAPAIAREAVRLLNWKLTKKPN", "aa_hexdigest": "9c41a12d09746eef9ba1da1d184c3d52", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449B2Q2", "query_cov": 0.9, "subject_cov": 0.9264705882352942, "identity": 0.516, "score": 135.0, "evalue": 2.28e-38, "valid": false, "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A449B2Q2" }, "pscc": { "uniref50_id": "UniRef50_A0A449B2Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "product": "Glycerol-3-phosphate dehydrogenase" }, "genes": [], "id": "CNOCLNGIAL_156", "locus": "GCA000143945_00116" }, { "type": "cds", "sequence": "contig_1", "start": 64062, "stop": 64175, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGCGAGCAAATGTTTAGCTTTAGCAGCACAGCAAAACAAAGTACAAATTTGCACTAATACTGAGGTTACTAATATTAGTAAGCAAGTTGATGGGACTTATTTAGTTTGA", "aa": "MLASKCLALAAQQNKVQICTNTEVTNISKQVDGTYLV", "aa_hexdigest": "74125aa4a908644cccc537cbd170a1fc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3997.6159, "isoelectric_point": 7.789279747009277 }, "id": "CNOCLNGIAL_157", "locus": "GCA000143945_00117" }, { "type": "cds", "sequence": "contig_1", "start": 64188, "stop": 64343, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGCGAACAATTAGAAGTACTAAAGCAAAGAGGCATTGCCAATCAAATTAATCTGGAAGATATTCAAATGCTCTCTAAAGAAGAAACCTTAAAGTTAGAGCCTTACGTTAATCCGGAAATTGTGGCTGGATTAAAAGTCGAGGGTAGCTGA", "aa": "MEREQLEVLKQRGIANQINLEDIQMLSKEETLKLEPYVNPEIVAGLKVEGS", "aa_hexdigest": "5b38d9513bf9171fc49916c66c7c925d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5824.6353, "isoelectric_point": 4.605061149597168 }, "id": "CNOCLNGIAL_158", "locus": "GCA000143945_00118" }, { "type": "cds", "sequence": "contig_1", "start": 64416, "stop": 64634, "strand": "-", "frame": 1, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00296496E8" ], "nt": "ATGGAAACAAGAGATGTTTTAATAGTTGGCGGTGGTGTAATAGGCTGTGCCACTGCTTATGAATTGAGCCAGTACAAGCTCAAAGTGACACTGGTAGAAAAGCACCATTATTTGGCACAAGAGACTAGCCATGCCAACAGTGGTGTTATCCACACTGGCATTGATCCCAATCCACACAAGTTAACTGCTAAATACAACATTTTGGGTAAAAAGCTCTGA", "aa": "METRDVLIVGGGVIGCATAYELSQYKLKVTLVEKHHYLAQETSHANSGVIHTGIDPNPHKLTAKYNILGKKL", "aa_hexdigest": "5e84aaeae96c19411d08c02039616fdf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00296496E8", "query_cov": 0.9583333333333334, "subject_cov": 0.92, "identity": 0.522, "score": 76.3, "evalue": 7.58e-17, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_UPI00296496E8" }, "pscc": { "uniref50_id": "UniRef50_UPI00296496E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00296496E8" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "CNOCLNGIAL_159", "locus": "GCA000143945_00119" }, { "type": "cds", "sequence": "contig_1", "start": 64817, "stop": 65080, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGAAAAGGGTTGGTCAAAAAAGGCCTTTTATTTTTTAGTGGCGTGAGTACTATGGCCGTCTTTTTAGTGTCTTGTGGTGCTACTAGAATTTGGGAATCTTCGATTCAACTGTTAGTTTCCAATGATGAAGCTACATTAGCTGACAAATCCTTTTCTGAGATGTCCTATGAAGGGATTAGAAGGTACTTTAGATCTCAAAAACATATTGAATTACCATCACCAAATAGTTCCCTGTTACAAGACGGCAACGGCCTTTGA", "aa": "MFGKGLVKKGLLFFSGVSTMAVFLVSCGATRIWESSIQLLVSNDEATLADKSFSEMSYEGIRRYFRSQKHIELPSPNSSLLQDGNGL", "aa_hexdigest": "7fd589358455c31304d46f75cc35efe1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9593.904499999991, "isoelectric_point": 7.894234275817872 }, "id": "CNOCLNGIAL_160", "locus": "GCA000143945_00120" }, { "type": "cds", "sequence": "contig_1", "start": 65150, "stop": 66163, "strand": "+", "frame": 2, "gene": "med", "product": "putative lipo MG040-like protein", "db_xrefs": [ "COG:COG1744", "COG:T", "GO:0005886", "KEGG:K07335", "SO:0001217", "UniRef:UniRef50_P47286", "UniRef:UniRef90_P75062" ], "nt": "GTGATTGTAGCTACTGGATTTAACCAACAAGAAGCACTACAAGCAATTAGCTCTGATGACCGTCGTTATTTAGCTGATAAAAACGACCTTGCTAAAGTTGGTTTCATTTTTGTCGATGGCCAAATTGAAAAGGAATATAACGTGATTAATAAAACGCCACAATTTAGATCAACCCCACTAAACATTTCATCCGTTGCCTTCCGCAGTGATGATGGTTCCTTCTTAACTGGAGTAGCAACTGCAGTTTATTTGAATTTAAATCAAGATTACTTTTTAAAGAAGAATGGAGCAACCAATAATAGTAGCCAAGATTTAACCGTTTCTGGTTTTGTCGGTGTTCCTATTCCTTCAACCCTTTCTTTTTTAAACGGTTTCCGTTTGGGCATTGCTTATTTTAATGAGGTGATTTACACCCATTTAAGTGATGCTGAAACAACATCTGATAACAAGAGTAATTCTTCAAGTGCTAGCAACTCTGTTTTAGTGCAACTTAAACAGATGCAAGGAAACGACAAAAAGATTAAAAAGATTAAATGGATATCTCCTAAACAAGGAAGCGATAATAATTCGAACCTCAGTATCGATGATCACAAGTCTGGTTCTTTCTCCAGCACTGAACCACGAGCAACAACTATCATTAATAATCTTTTAGACAAGGGTGTGAGTGCCATTATTCCTGTTGCCGGACCGCAAGTAAACTTAGCAGTTAACGAGGTGGCACGTCGCAAAGCGCACACTGCTATTATTGGTGTTGATAGTGCGCAAGAATTATTAGATATTAACCAAGATGCACCTGATAAAGATCAGTTAATCAAAGGTAATAAAAAAATTATTCCTTTCTCTTCTATTAAAGCCTTGGATGTTGCCATTGAAAACATGTTGATTGCCATTCAAAAAGGCAGTGATAATAATGGATATAAAGGATTTGGTTATAACAACATTGGTACGGTTGGAACTAGTTCAGTAGGTATTAGTGAGGCTGGTTATGAATTCTTAATTGATCCAGTTTTTTGA", "aa": "MIVATGFNQQEALQAISSDDRRYLADKNDLAKVGFIFVDGQIEKEYNVINKTPQFRSTPLNISSVAFRSDDGSFLTGVATAVYLNLNQDYFLKKNGATNNSSQDLTVSGFVGVPIPSTLSFLNGFRLGIAYFNEVIYTHLSDAETTSDNKSNSSSASNSVLVQLKQMQGNDKKIKKIKWISPKQGSDNNSNLSIDDHKSGSFSSTEPRATTIINNLLDKGVSAIIPVAGPQVNLAVNEVARRKAHTAIIGVDSAQELLDINQDAPDKDQLIKGNKKIIPFSSIKALDVAIENMLIAIQKGSDNNGYKGFGYNNIGTVGTSSVGISEAGYEFLIDPVF", "aa_hexdigest": "9787d68f39a84e05c118fc11d53cf86d", "start_type": "GTG", "rbs_motif": null, "genes": [ "med" ], "truncated": "both", "pseudogene": { "start": 64850, "stop": 66466, "observations": { "mutation": [ 65078, 66161 ], "stop": [ 65078, 66161 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75062", "query_cov": 1.0, "subject_cov": 0.512937595129376, "identity": 0.997, "score": 639.0, "evalue": 5.84e-226, "gene_start": 1, "gene_end": 337, "reference_start": 112, "reference_end": 448, "reference_sequence": "MFGKGLVKKSLLFFSGVSTMAVFLVSCGATRIWESSIQLLVSNDEATLADKSFSEMSYEGIRRYFRSQKHIELPSPNSSLLQDGNGLWKRPGRTLSDRIATFKNIKNDGSDVIVATGFNQQEALQAISSDDRRYLADKNDLAKVGFIFVDGQIEKEYNVINKTPQFRSTPLNISSVAFRSDDGSFLTGVATAVYLNLNQDYFLKKNGATNNSSQDLTVSGFVGVPIPSTLSFLNGFRLGIAYFNEVIYTHLSDAETTSDNKSNSSSASNSVLVQLKQMQGNDKKIKKIKWISPKQGSDNNSNLSIDDHKSGSFSSTEPRATTIINNLLDKGVSAIIPVAGPQVNLAVNEVARRKAHTAIIGVDSAQELLDINQDAPDKDQLIKGNKKIIPFSSIKALDVAIENMLIAIQKGSDNNGYKGFGYNNIGTVGTSSVGISEAGYEFLIDPVFWKTTQSQGKSMATNMTNLKRLSSDDTNTKKALKEVSTHKNGSDKDGIIGKYSNLLTKKSTTVTAVAQKSMTDNNSGTEQKKNLSEVDTKKKEKESKGKTQSNGQDSGQQNGKETNDIISKYSKLLTMTTMNNKVMSSKKQSSDDNSFKKTSENGDWVIKGDELTKKKSTELPAFAKGADYPTFPTEAVSVINGSTALDGKGFKWSFKQI" }, "paralog": false, "identity": 0.9962825278810409, "score": 1038.0, "evalue": 0.0, "description": "Internal stop codon at 65078, 66161. Nonsense mutation around 65078, 66161.", "psc": { "uniref90_id": "UniRef90_P75062", "gene": "Med", "product": "Uncharacterized lipoprotein MG040 homolog", "uniref50_id": "UniRef50_P47286", "cog_id": "COG1744", "cog_category": "T", "kegg_orthology_id": "K07335", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75062", "UniRef:UniRef50_P47286", "COG:COG1744", "COG:T", "KEGG:K07335", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47286", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47286" ], "product": "Uncharacterized lipoprotein MG040" } }, "id": "CNOCLNGIAL_161", "locus": "GCA000143945_00121" }, { "type": "cds", "sequence": "contig_1", "start": 66191, "stop": 66628, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCACAAATATGACTAATTTAAAACGTTTATCATCTGATGATACAAACACTAAGAAAGCATTAAAAGAGGTAAGCACCCATAAAAATGGTAGTGATAAAGATGGGATTATTGGTAAATATTCCAATTTATTAACTAAAAAAAGCACAACAGTAACTGCTGTTGCTCAAAAATCTATGACAGACAATAATAGTGGAACTGAGCAAAAAAAGAATCTTTCTGAAGTAGATACTAAGAAGAAAGAGAAAGAAAGTAAAGGTAAAACTCAAAGTAATGGTCAGGACAGTGGCCAACAAAACGGAAAAGAAACAAACGATATTATTTCTAAGTATTCTAAGCTGTTAACTATGACAACTATGAACAATAAAGTAATGTCATCTAAAAAACAAAGTAGCGATGATAACAGTTTTAAAAAGACTTCAGAAAACGGAGACTGA", "aa": "MATNMTNLKRLSSDDTNTKKALKEVSTHKNGSDKDGIIGKYSNLLTKKSTTVTAVAQKSMTDNNSGTEQKKNLSEVDTKKKEKESKGKTQSNGQDSGQQNGKETNDIISKYSKLLTMTTMNNKVMSSKKQSSDDNSFKKTSENGD", "aa_hexdigest": "3e707f1c1aff50d4d726a2f89400c525", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15895.454799999989, "isoelectric_point": 9.602001762390138 }, "id": "CNOCLNGIAL_162", "locus": "GCA000143945_00122" }, { "type": "cds", "sequence": "contig_1", "start": 66808, "stop": 67074, "strand": "+", "frame": 1, "gene": "ptsH", "product": "phosphocarrier protein HPr", "db_xrefs": [ "BlastRules:WP_000411080", "BlastRules:WP_010874410", "COG:COG1925", "COG:TG", "GO:0005737", "GO:0009401", "RefSeq:WP_010874410.1", "SO:0001217", "UniParc:UPI00001328F5", "UniRef:UniRef100_P75061", "UniRef:UniRef50_P75061", "UniRef:UniRef90_P75061" ], "nt": "ATGAAGAAGATTCAAGTAGTCGTTAAAGACCCTGTGGGCATTCACGCCCGTCCTGCTAGTATTATTGCTGGTGAGGCTAATAAATTTAAATCGGAATTAAAGCTAGTATCACCAAGCGGCGTTGAAGGTAACATCAAGTCGATCATAAACTTAATGTCTTTAGGCATTAAGCAAAACGACCACATTACCATCAAGGCAGAAGGAACTGATGAAGAAGAGGCACTAAATGCTATTAAAGCAGTTTTAGAAAAACACCAAGTTATTTAA", "aa": "MKKIQVVVKDPVGIHARPASIIAGEANKFKSELKLVSPSGVEGNIKSIINLMSLGIKQNDHITIKAEGTDEEEALNAIKAVLEKHQVI", "aa_hexdigest": "ea7f7abc14674640ebb74226629ff732", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001328F5", "ncbi_nrp_id": "WP_010874410.1", "uniref100_id": "UniRef100_P75061", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001328F5", "RefSeq:WP_010874410.1", "UniRef:UniRef100_P75061" ] }, "ips": { "uniref100_id": "UniRef100_P75061", "uniref90_id": "UniRef90_P75061" }, "psc": { "uniref90_id": "UniRef90_P75061", "gene": "ptsH", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_P75061", "cog_id": "COG1925", "cog_category": "TG", "go_ids": [ "GO:0005737", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75061" ], "product": "Phosphocarrier protein HPr" }, "expert": [ { "type": "expert_proteins", "source": "BlastRules", "rank": 80, "id": "WP_010874410", "gene": "ptsH", "product": "phosphocarrier protein HPr", "query_cov": 1.0, "subject_cov": 1.0, "identity": 1.0, "score": 168.0, "evalue": 1.27e-56, "db_xrefs": [ "BlastRules:WP_000411080", "BlastRules:WP_010874410" ] } ], "genes": [ "ptsH" ], "id": "CNOCLNGIAL_163", "locus": "GCA000143945_00123" }, { "type": "cds", "sequence": "contig_1", "start": 67151, "stop": 67276, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTACTACGACGACGAGGATGAATATGACACTTCTAATTCTGGTGGTGTCGTAGATTTAGCATTTTTAGCACTAATAGTAGTTTTTCTATTTACCGCTTTTATTTCTTGGTTTGGGGCATGA", "aa": "MLYYDDEDEYDTSNSGGVVDLAFLALIVVFLFTAFISWFGA", "aa_hexdigest": "722943f8e320b6775e88410304ac02b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4616.109799999998, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_164", "locus": "GCA000143945_00124" }, { "type": "cds", "sequence": "contig_1", "start": 68601, "stop": 68831, "strand": "+", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_054", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75060", "UniRef:UniRef50_UPI001FCEF156", "UniRef:UniRef90_P75060", "UniRef:UniRef90_UPI001FCEF156" ], "nt": "ATGAAGATTAAATATTTCTTTATTCCCTTGTTTTCTTCAGTTATATTATTTAGTGCCTGTTCTTCTATTCAGTCTGATCTTAGGAATCTTATTAAAGAAACAACTGGCAAAGATTTTGACGTATCTAAATTAATTAAAACCTCTGAAGGAAGAAAAAACTTAATAAATTCTCTCAAAAAGTCTTACGAATCTAAGCCCGATGAAACAGCTAGCTTATTACTAATTGCTTGA", "aa": "MKIKYFFIPLFSSVILFSACSSIQSDLRNLIKETTGKDFDVSKLIKTSEGRKNLINSLKKSYESKPDETASLLLIA", "aa_hexdigest": "2d543206ab483ba012141d764e9c031f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCEF156", "query_cov": 0.9473684210526315, "subject_cov": 0.9863013698630136, "identity": 1.0, "score": 137.0, "evalue": 4.77e-41, "valid": true, "uniref50_id": "UniRef50_UPI001FCEF156" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCEF156", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCEF156" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 68583, "stop": 68972, "observations": { "mutation": [ 68829 ], "stop": [ 68829 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75060", "query_cov": 1.0, "subject_cov": 0.5891472868217055, "identity": 0.987, "score": 142.0, "evalue": 3.79e-42, "gene_start": 1, "gene_end": 76, "reference_start": 7, "reference_end": 82, "reference_sequence": "RYLSNLMKIKYFFIPLFSSAILFSACSSIQSDLRNLIKETTGKDFDVSKLIKTSEGRKNLINSLKKSYESKPDETASLLLIAWKQSAEMGEIGFDDIKEGGIYTRDNDPFKLEQKVEYFNMEYKILVMF" }, "paralog": false, "identity": 0.9844961240310077, "score": 249.0, "evalue": 9.96e-87, "description": "Internal stop codon at 68829. Nonsense mutation around 68829.", "psc": { "uniref90_id": "UniRef90_P75060", "product": "Uncharacterized lipoprotein MPN_054", "uniref50_id": "UniRef50_P75060", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75060", "UniRef:UniRef50_P75060", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75060", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75060" ], "product": "Uncharacterized lipoprotein MPN_054" } }, "id": "CNOCLNGIAL_165", "locus": "GCA000143945_00125" }, { "type": "cds", "sequence": "contig_1", "start": 68847, "stop": 68972, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGAAATAGGTTTTGATGATATTAAAGAAGGAGGAATTTATACCAGAGACAATGATCCTTTCAAATTGGAACAAAAGGTTGAGTACTTCAATATGGAATACAAAATTTTAGTGATGTTTTAA", "aa": "MGEIGFDDIKEGGIYTRDNDPFKLEQKVEYFNMEYKILVMF", "aa_hexdigest": "43d7734811b693ec99330b72d074c09f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4924.5788999999995, "isoelectric_point": 4.399986076354981 }, "id": "CNOCLNGIAL_166", "locus": "GCA000143945_00126" }, { "type": "cds", "sequence": "contig_1", "start": 70379, "stop": 71266, "strand": "+", "frame": 2, "gene": "potA", "product": "Spermidine/putrescine import ATP-binding protein PotA", "db_xrefs": [ "COG:COG3839", "COG:G", "EC:7.6.2.11", "GO:0005524", "GO:0005886", "GO:0015417", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75059", "UniRef:UniRef90_P75059" ], "nt": "TTGAACGAACGTTTTTTAATTGAAATCGAAGGTTTAAACAAAACTTTCGATGATGGTTTTGTATCAGTACGTGATATTAACCTCAAAATTAAGAAGGGCGAATTTATTACGATTCTCGGTCCTTCTGGTTGTGGCAAAACAACTACTCTTAGGTTGTTAGCAGGTTTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTCTTGACATTAAAGACTTGCCCATTCATAAGCGTCCCTTTGCTACTGTCTTCCAAGACTATGCCTTGTTTTCACACTTAACTGTGTACAAGAACATTGCTTATGGCTTGAAGGCAATGTACACAAAGCTGGATCCGATTGATAAGCTAGTGGAACAATACCACCAAAGCTTACTGGACAAGCAACACCGCTTGCACAAGAGGATTGAACGCTTAGAAAAAAGTAATGCTAATCCACAACTGTTAGAACAACTTAAACAAACGGTTGTCGTTCAACAAAAACAGTTTAAACAACAAACAGAGACTTTTAAACAAAAGGAAAATGCTCGACGTGATGCCATTCAACAACGCTTGGTGCAACTTACCAAGGAATGGGAAAGCTTATCAAAGCAGAAGTTGCAACAATTAGAAGCGGAGAAAAAGTTGTTAGATAAAAAGTTTGAACAAACTGAGCGCAAATACCAAAAGGATGCTTGGATGGCAACCCACAGTGAAATGCGTTTAAAGCAGTTCAAACAAGAGGTGTTAGCTTTAAAACAACTAATTAAAACTAAGTTCAAACAAAATGAACCAGTTGACAAGTTGCAGTTAAAGCTGCAAACCTTAAAGCAAAAGTATGCTGCTAAAAGACAAATTGATAAGGAATACGATAAGTTGGTCTTAGCCTACAACAAGAAGGACTATTGA", "aa": "MNERFLIEIEGLNKTFDDGFVSVRDINLKIKKGEFITILGPSGCGKTTTLRLLAGFEDPTYGKIKVNGLDIKDLPIHKRPFATVFQDYALFSHLTVYKNIAYGLKAMYTKLDPIDKLVEQYHQSLLDKQHRLHKRIERLEKSNANPQLLEQLKQTVVVQQKQFKQQTETFKQKENARRDAIQQRLVQLTKEWESLSKQKLQQLEAEKKLLDKKFEQTERKYQKDAWMATHSEMRLKQFKQEVLALKQLIKTKFKQNEPVDKLQLKLQTLKQKYAAKRQIDKEYDKLVLAYNKKDY", "aa_hexdigest": "3c064fba9f648766d04a231e95ea2d8b", "start_type": "TTG", "rbs_motif": "AAAAA", "genes": [ "potA" ], "truncated": "3-prime", "pseudogene": { "start": 70379, "stop": 71569, "observations": { "mutation": [ 71264 ], "stop": [ 71264 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75059", "query_cov": 1.0, "subject_cov": 0.5267857142857143, "identity": 1.0, "score": 550.0, "evalue": 2.35e-193, "gene_start": 1, "gene_end": 295, "reference_start": 1, "reference_end": 295, "reference_sequence": "MNERFLIEIEGLNKTFDDGFVSVRDINLKIKKGEFITILGPSGCGKTTTLRLLAGFEDPTYGKIKVNGLDIKDLPIHKRPFATVFQDYALFSHLTVYKNIAYGLKAMYTKLDPIDKLVEQYHQSLLDKQHRLHKRIERLEKSNANPQLLEQLKQTVVVQQKQFKQQTETFKQKENARRDAIQQRLVQLTKEWESLSKQKLQQLEAEKKLLDKKFEQTERKYQKDAWMATHSEMRLKQFKQEVLALKQLIKTKFKQNEPVDKLQLKLQTLKQKYAAKRQIDKEYDKLVLAYNKKDYWTSYWETYSLQQQEAFEKRYLSRKLTKQEQHQKVCAVIELVGLKGNEDKLPEELSGGMKQRVALARSLVIEPDILLLDEPLSALDAKVRKNLQKELQKIHQQSGLTFILVTHDQEEALVLSNRIVVMNEGNILQVGSPADIYDSPKTEWIANFIGQANIFKGTYLGDLKIKLHSGEVIKTDVDNNYVVGKEYKILIRPEDFDIVPKNKGFFNVRVIDKTYKGLLWKITTKLVDDTIVDLESVNDIEVDKTFGVTFDPIDVHLMEV" }, "paralog": false, "identity": 0.9949494949494949, "score": 775.0, "evalue": 5.44e-284, "description": "Internal stop codon at 71264. Nonsense mutation around 71264.", "psc": { "uniref90_id": "UniRef90_P75059", "gene": "potA", "product": "Spermidine/putrescine import ATP-binding protein PotA", "ec_ids": [ "7.6.2.11" ], "uniref50_id": "UniRef50_P75059", "cog_id": "COG3839", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0015417", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75059", "EC:7.6.2.11", "UniRef:UniRef50_P75059", "COG:COG3839", "COG:G", "GO:0005886", "GO:0015417", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75059", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75059" ], "product": "Spermidine/putrescine import ATP-binding protein PotA" } }, "id": "CNOCLNGIAL_167", "locus": "GCA000143945_00127" }, { "type": "cds", "sequence": "contig_1", "start": 71363, "stop": 71938, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F197FDC", "UniRef:UniRef90_UPI001F197FDC" ], "nt": "GTGTGTGCTGTAATTGAGTTAGTTGGTTTAAAGGGTAACGAGGACAAACTCCCAGAAGAATTGTCTGGGGGAATGAAGCAACGGGTAGCCTTAGCGCGTTCCTTGGTAATTGAACCAGACATTCTCTTATTGGATGAACCCTTGTCTGCTTTAGATGCTAAGGTACGTAAAAACTTGCAAAAGGAATTGCAAAAGATTCACCAACAAAGTGGTTTAACCTTTATCTTAGTTACTCACGACCAGGAAGAAGCCTTGGTCTTGTCCAACCGCATTGTGGTAATGAATGAGGGTAACATTCTCCAAGTTGGTAGTCCGGCTGATATTTACGATTCACCCAAAACCGAGTGGATTGCTAACTTCATTGGTCAAGCCAATATCTTTAAGGGTACTTACCTAGGTGACCTTAAGATTAAACTGCACAGCGGTGAAGTGATTAAAACTGATGTGGACAATAACTATGTGGTTGGTAAGGAATACAAAATCCTAATTCGTCCCGAAGACTTTGACATTGTGCCCAAAAACAAAGGCTTCTTTAATGTTCGGGTAATTGATAAAACCTACAAAGGATTACTCTGA", "aa": "MCAVIELVGLKGNEDKLPEELSGGMKQRVALARSLVIEPDILLLDEPLSALDAKVRKNLQKELQKIHQQSGLTFILVTHDQEEALVLSNRIVVMNEGNILQVGSPADIYDSPKTEWIANFIGQANIFKGTYLGDLKIKLHSGEVIKTDVDNNYVVGKEYKILIRPEDFDIVPKNKGFFNVRVIDKTYKGLL", "aa_hexdigest": "c9980fc5d079bbf83c983fbc1d91f765", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "both", "pseudogene": { "start": 71234, "stop": 72061, "observations": { "mutation": [ 71264, 71936 ], "stop": [ 71264, 71936 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F197FDC", "query_cov": 0.9842931937172775, "subject_cov": 0.6836363636363636, "identity": 0.883, "score": 337.0, "evalue": 4.74e-115, "gene_start": 4, "gene_end": 191, "reference_start": 47, "reference_end": 234, "reference_sequence": "MVVAYNKKDYWTSYWETYTLQQKEAFEKRYLSRKLTKAEQNKKVSDVIEMVGLKGKEDRLPDELSGGMKQRVALARSLVVEPEILLLDEPLSALDAKVRKNLQKELQQIHKKSGLTFILVTHDQEEALVLSDRIVVMNEGNILQVGNPVDIYDSPKTEWIANFIGQANIFKGTYLGEKKIQLQSGEIIQTDVDNNYVVGKQYKILIRPEDFDLVPENKGFFNVRVIDKNYKGLLWKITTQLKDNTIVDLESVNEVDVNKTFGVLFDPIDVHLMEV" }, "paralog": false, "identity": 0.850909090909091, "score": 473.0, "evalue": 3.87e-171, "description": "Internal stop codon at 71264, 71936. Nonsense mutation around 71264, 71936.", "psc": { "uniref90_id": "UniRef90_UPI001F197FDC", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI001F197FDC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F197FDC", "UniRef:UniRef50_UPI001F197FDC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F197FDC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F197FDC" ], "product": "ABC transporter ATP-binding protein" } }, "id": "CNOCLNGIAL_168", "locus": "GCA000143945_00128" }, { "type": "cds", "sequence": "contig_1", "start": 72063, "stop": 72239, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTATCGAAAAAATACCTCTTAGCTGTACCATTCTTTGTTTTAATGGTCATCTTCTTTGTAGTACCAATGGCATGGATTATTGTTAGTGGACTCCAAAATGAAAACGGTGCAAGTATAACGGAAAAGTACCAACCCTTAGTTGGTGGTTATTCTTTCTTTCAGAGTTTTTGA", "aa": "MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSF", "aa_hexdigest": "175b6ae90ee0fa8116a19eac8dbc2b8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6595.827299999999, "isoelectric_point": 9.224861717224119 }, "id": "CNOCLNGIAL_169", "locus": "GCA000143945_00129" }, { "type": "cds", "sequence": "contig_1", "start": 72261, "stop": 72923, "strand": "+", "frame": 3, "gene": "potB", "product": "Spermidine/putrescine transport system permease PotB-like protein", "db_xrefs": [ "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47289", "UniRef:UniRef90_P75058" ], "nt": "GTGACAGTACTAGTAGCCTTACTGGTTGCCTTTCCCTTTTGTTACTTTTTATCACAAAGTAAAAACAAGGTATTCCGCAGTTTTGTGATTGCCTTAGCTACTGCTCCCATATGGAGTAGTTTTCTCATTAAGTTAATTGGGTTAAAAACACTGTTGGACTTAGTTTTGGGGTTAGCGTTGAACCGGGTGGGTGATAATAACCTCACCTTTGGTAGTGGTTATACCTTGATTGGGATGATCTACCTGTTCACCCCCTTCATGTTTTTACCACTTTACAACAACTTTTGTATCTTACCGAAAAACTTAATCTTAGCTAGTCAAGATTTGGGTTACAACTGGATTACGAGTTTTATTAAGGTAGTTATTCCCTTTTCTAAAACTGCCATTCTGTCAGGGATTGCGTTAACTTTCTTTCCGTCTTTAACCTCGGTAGCGATTGCCCAGTTCTTGGACAACTCGAATCAAAATAACACCTTAGGTAACTATGTCTTTACTTTAGGTAACAATGGTTATGACAGTGCGATTGAGCGCGGCCGTGCTTCTGGTGCCATTATTATTGCGGCTTTAATTACCTTTGCCTTTTACTTTGTGGTGATCTTTGCTCCAAGAATAGTGCGCTTAATCCAGACTAAGTGTTTGGAATACAGGAGGGTTAATGTTTAA", "aa": "MTVLVALLVAFPFCYFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNNFCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSAIERGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLEYRRVNV", "aa_hexdigest": "6747efff365e1f795a9105a13bf92286", "start_type": "GTG", "rbs_motif": null, "genes": [ "potB" ], "truncated": "5-prime", "pseudogene": { "start": 72063, "stop": 72923, "observations": { "mutation": [ 72237, 72249 ], "stop": [ 72237, 72249 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75058", "query_cov": 0.9590909090909091, "subject_cov": 0.7377622377622378, "identity": 0.995, "score": 403.0, "evalue": 1.46e-140, "gene_start": 10, "gene_end": 220, "reference_start": 76, "reference_end": 286, "reference_sequence": "MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSFWTSLWTATVTVLVALLVAFPFCYFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNNFCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSAIERGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV" }, "paralog": false, "identity": 0.9335664335664335, "score": 518.0, "evalue": 2.74e-188, "description": "Internal stop codon at 72237, 72249. Nonsense mutation around 72237, 72249.", "psc": { "uniref90_id": "UniRef90_P75058", "gene": "potB", "product": "Spermidine/putrescine transport system permease protein PotB homolog", "uniref50_id": "UniRef50_P47289", "cog_id": "COG1176", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75058", "UniRef:UniRef50_P47289", "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47289" ], "product": "Spermidine/putrescine transport system permease protein PotB homolog" } }, "id": "CNOCLNGIAL_170", "locus": "GCA000143945_00130" }, { "type": "cds", "sequence": "contig_1", "start": 72984, "stop": 73151, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGAGTTGAGTAAAGGGATGGCAAAGTCCGCTTCCCCTAATCTTAGATATGCTGACTTGGCATCCGGGTTAGGGTTTAAAACATTACCAAAGTCTAAGACAATGTTACCCCTAGTCGAAGAACCGTTAAAACTAACTAAGACAACTATTATTAAAGGGAGGTAG", "aa": "MTELSKGMAKSASPNLRYADLASGLGFKTLPKSKTMLPLVEEPLKLTKTTIIKGR", "aa_hexdigest": "462c9d9167240b310cef5a0cd810fa7d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5963.084199999999, "isoelectric_point": 10.092283821105958 }, "id": "CNOCLNGIAL_171", "locus": "GCA000143945_00131" }, { "type": "cds", "sequence": "contig_1", "start": 73195, "stop": 73650, "strand": "+", "frame": 1, "gene": "potC", "product": "Spermidine/putrescine transport system permease PotC-like protein", "db_xrefs": [ "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47290", "UniRef:UniRef90_P75057" ], "nt": "ATGACTGCCTTTGCTTTATTGCGATCCAGGCAATGGCTCAATAAAACAGTCTTTGGGATTGCTAACTTTTCTCTAGCAACTCCCGATATTATTACCGGGATATCGCTAGTGTTGTTATTTGCCAACACCTGGTTGAGCTTTAACCAACAGTTAGGTTTCTTTACTATTATTAGTTCCCACATCTCCTTTTCGGTGCCTTATGCCTTGGTGTTAATTTACCCAAAGATGCAAAAGTTAAACCGTAACTTAATCCTAGCGAGTCAGGATTTGGGTTACTCACCCATTGCTACCTTCTTTCACATTACCTTACCTTACTTACTACCGAGTATCTTGTCAGCCATTCTAGTAGTGTTTGCCACGAGCTTTGATGACTATGTGATTACTTCCTTAGTTCAAGGTTCAGTTAAAACAGTCGCCAGTGAGCTCTATTCCTTTAGAAAGGGGATTAAGGCCTGA", "aa": "MTAFALLRSRQWLNKTVFGIANFSLATPDIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPYALVLIYPKMQKLNRNLILASQDLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRKGIKA", "aa_hexdigest": "f2806938ae3e849e0fff7f1ce6a673f7", "start_type": "ATG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "both", "pseudogene": { "start": 72916, "stop": 73776, "observations": { "mutation": [ 72943, 73648, 73762 ], "stop": [ 72943, 73648, 73762 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75057", "query_cov": 1.0, "subject_cov": 0.527972027972028, "identity": 1.0, "score": 283.0, "evalue": 1.89e-94, "gene_start": 1, "gene_end": 151, "reference_start": 94, "reference_end": 244, "reference_sequence": "MFKMKSCKLWLRGSFFVIVLVLIYLPLIIVVLVSFNGSSTRGNIVLDFGNVLNPNPDAKSAYLRLGEADFAIPLLNSVIIGLITVIVSIPIAIMTAFALLRSRQWLNKTVFGIANFSLATPDIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPYALVLIYPKMQKLNRNLILASQDLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRKGIKAWAIAFGTILILVSILAVLLVTLHKYLRFKHKEMLRVKQWKNS" }, "paralog": false, "identity": 0.986013986013986, "score": 533.0, "evalue": 3.16e-195, "description": "Internal stop codon at 72943, 73648, 73762. Nonsense mutation around 72943, 73648, 73762.", "psc": { "uniref90_id": "UniRef90_P75057", "gene": "potC", "product": "Spermidine/putrescine transport system permease protein PotC homolog", "uniref50_id": "UniRef50_P47290", "cog_id": "COG1177", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75057", "UniRef:UniRef50_P47290", "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47290" ], "product": "Spermidine/putrescine transport system permease protein PotC homolog" } }, "id": "CNOCLNGIAL_172", "locus": "GCA000143945_00132" }, { "type": "cds", "sequence": "contig_1", "start": 73761, "stop": 74045, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAGTTAAAGTATTGGTTTAGTCTAATGGGACTAACCATGTCAGTTGGGTTAACTGCTTGTAGTTCCAACCAGTTTGTGTTTGCTAACTTTGAGTCGTATGTGTCGCCCTTACTTTTAGAAAGGGCAGAAGCCAAGCAACCAATGACCTTTCTTACTTATCCTACTAACGAAAAGTTAATTAACGGATTTGCTAACAATACCTATACGGTAGCAGTGGCCTCGAGTTATGCTGTGAGTGAACTGCAGCAACAAGGCCACTTACTACCAATTGATTGA", "aa": "MKKQLKYWFSLMGLTMSVGLTACSSNQFVFANFESYVSPLLLERAEAKQPMTFLTYPTNEKLINGFANNTYTVAVASSYAVSELQQQGHLLPID", "aa_hexdigest": "6dc6f5b0027591970040725680a8f495", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10535.000199999991, "isoelectric_point": 6.545373725891113 }, "id": "CNOCLNGIAL_173", "locus": "GCA000143945_00133" }, { "type": "cds", "sequence": "contig_1", "start": 74355, "stop": 75215, "strand": "+", "frame": 3, "gene": "potD", "product": "putative lipo MG045-like protein", "db_xrefs": [ "COG:COG0687", "COG:E", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75056", "UniRef:UniRef90_P75056" ], "nt": "GTGCACCATGAGGTTAAGAATACCACGGTAGATGTTAATCCTACAGGTAGCACCTTAAACTACTTTGGCAACGTTTACGAAAGTTTTGCAAATTTAGGTCTAAAACGTGATAACCTCAACACGCTCTTTGTCAATTCGGACTCCAACATTATTATTAATGAATTAGCTAATGGCAGAAGACAAGGTGGCATTGTTTACAATGGTGATGCCGTATATGCCGCTTTGGGCGGTGATCTCCGCGATGAAATTAATGAGAATAACTTGCCCAACGGCGATAACTTCCACATTGTGCAACCGAAACACTCGCCTGTGGCCCTAGACTTTCTCATTATTAACCAGCAACAAACTCACTTTAGAGATGCTGCACACCAGTTAATTTACCAGTTGGCCTTAGAAGGCGCTGATCAAACAGCAGAAGAATTATTAAAAACTGATGAAGAGAAAGGTACCAGTGATGAGGACTATTACACTTATGGTGCAATGCAGAATTTTAGTTACGTTAACTATGTTTCGCCTTTAAAGAATATTTCTGATGAAACCACTGGGATTGTGTTTAAGGAAAACAAACAAGCTGATACTAAGCGAGTTGTCAAACAACAAAGTCAATCAGAACAACAATCTGAATCAGCAGAAAAGGAAGAAACGGAACAAGATGATTTCTACACTGCTACTCTCAAAAGTTTGTTGAAAGCGGACAGTTTAGATGACAAAGCTAAGAAGTTAGTAGACACAATTAAAAAGACTTACAAGATCAAAAAAGCCGACAATATTAATTGGGCTAACTTAATCGAGAAACCAATTACCCCATTACAAAGGAGTAATTTAACATTATCGTGGCTTGACTTTAAGCAACGCTTTTGA", "aa": "MHHEVKNTTVDVNPTGSTLNYFGNVYESFANLGLKRDNLNTLFVNSDSNIIINELANGRRQGGIVYNGDAVYAALGGDLRDEINENNLPNGDNFHIVQPKHSPVALDFLIINQQQTHFRDAAHQLIYQLALEGADQTAEELLKTDEEKGTSDEDYYTYGAMQNFSYVNYVSPLKNISDETTGIVFKENKQADTKRVVKQQSQSEQQSESAEKEETEQDDFYTATLKSLLKADSLDDKAKKLVDTIKKTYKIKKADNINWANLIEKPITPLQRSNLTLSWLDFKQRF", "aa_hexdigest": "394d4ce4bd64700c3123ea2c05a6d377", "start_type": "GTG", "rbs_motif": null, "genes": [ "potD" ], "truncated": "5-prime", "pseudogene": { "start": 74055, "stop": 75215, "observations": { "mutation": [ 74250 ], "stop": [ 74250 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75056", "query_cov": 1.0, "subject_cov": 0.5896907216494846, "identity": 0.972, "score": 531.0, "evalue": 3.44e-187, "gene_start": 1, "gene_end": 286, "reference_start": 199, "reference_end": 484, "reference_sequence": "MKKQLKYWFSLMGLTMSVGLTACSSNQFVFANFESYVSPLLLERAEAKQPMTFLTYPTNEKLINGFANNTYTVAVASSYAVSELQQQGHLLPIDWAKFNLKKTQNGSNQATIQNKEDAKELFTKEIGDISGELLNWGVPYFLQDLVFVYRGEKIQELEGQDVTWSTIIKAIVNHKDRFNNNRLALIDDARTIFSLANVVHHEVKNTTVDVNPTGSTLNYFGNVYESFANLGLKRDNLNTLFVNSDSNIIINELANGRRQGGIVYNGDAVYAALGGDLRDEINENNLPNGDNFHIVQPKHSPVALDFLIINQQQTHFRDAAHQLIYQLALEGADQTAEELLKTDEEKGTSDEDYYTYGAMQNFSYVNYVSPLKNISDETTGIVFKENKQADTKQVVKQQSQSEQQSESAEKEETEQDDFYTATLKSLLKADSLDDKAKKLVDTIKKTYKIKKADNINWANLIEKPITPLQRSNLTLSWLDFKQRFW" }, "paralog": false, "identity": 0.9792746113989638, "score": 751.0, "evalue": 4.61e-276, "description": "Internal stop codon at 74250. Nonsense mutation around 74250.", "psc": { "uniref90_id": "UniRef90_P75056", "gene": "PotD", "product": "Uncharacterized lipoprotein MG045 homolog", "uniref50_id": "UniRef50_P75056", "cog_id": "COG0687", "cog_category": "E", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75056", "UniRef:UniRef50_P75056", "COG:COG0687", "COG:E", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75056" ], "product": "Uncharacterized lipoprotein MG045 homolog" } }, "id": "CNOCLNGIAL_174", "locus": "GCA000143945_00134" }, { "type": "cds", "sequence": "contig_1", "start": 75218, "stop": 75832, "strand": "+", "frame": 2, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711", "SO:0001217", "UniRef:UniRef50_P75055", "UniRef:UniRef90_P75055" ], "nt": "ATGGAACAACCTTTGTGTATCTTGGGAATTGAAACCACTTGTGATGACACTAGCATAGGGGTAATCACTGAGAGTAAGGTACAAGCACACATTGTTTTATCATCCGCCAAATTACACGCCCAAACTGGTGGCGTAGTGCCCGAAGTTGCAGCTAGGAGTCACGAACAAAACTTGCTTAAAGCACTGCAACAATCAGGGGTTGTTTTAGAACAAATTACTCACATTGCTTATGCCGCTAATCCGGGATTACCCGGTTGTCTGCATGTGGGAGCTACCTTTGCGCGCAGTTTAAGCTTCTTATTGGACAAACCCTTATTGCCCATCAACCACCTTTATGCCCACATCTTCTCGGCCTTAATTGACCAAGATATTAATCAGTTAAAACTCCCCGCTTTAGGCTTAGTGGTGTCCGGGGGGCACACTGCCATTTACCTAATTAAGTCCTTGTTTGACTTGGAGTTAATTGCTGAGACTAGCGATGATGCGATTGGCGAAGTTTATGATAAGGTGGGCCGCGCTATGGGTTTTCCTTATCCAGCTGGACCCCAGTTAGATAGTTTGTTTCAACCGGAGTTAGTTAAGTCACATTATTTCTTTCGTCCTTCCACGAAGTGA", "aa": "MEQPLCILGIETTCDDTSIGVITESKVQAHIVLSSAKLHAQTGGVVPEVAARSHEQNLLKALQQSGVVLEQITHIAYAANPGLPGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSALIDQDINQLKLPALGLVVSGGHTAIYLIKSLFDLELIAETSDDAIGEVYDKVGRAMGFPYPAGPQLDSLFQPELVKSHYFFRPSTK", "aa_hexdigest": "4be1fae36d08950b16846e51b5ffc889", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 75218, "stop": 76135, "observations": { "mutation": [ 75830 ], "stop": [ 75830 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75055", "query_cov": 1.0, "subject_cov": 0.6394984326018809, "identity": 1.0, "score": 407.0, "evalue": 3.64e-142, "gene_start": 1, "gene_end": 204, "reference_start": 1, "reference_end": 204, "reference_sequence": "MEQPLCILGIETTCDDTSIGVITESKVQAHIVLSSAKLHAQTGGVVPEVAARSHEQNLLKALQQSGVVLEQITHIAYAANPGLPGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSALIDQDINQLKLPALGLVVSGGHTAIYLIKSLFDLELIAETSDDAIGEVYDKVGRAMGFPYPAGPQLDSLFQPELVKSHYFFRPSTKWTKFSYSGLKSQCFTKIKQLRERKGFNPQTHDWNEFASNFQATIIDHYINHVKDAIQQHQPQMLLLGGGVSANKYLREQVTQLQLPYLIAPLKYTSDNGAMIGFYANLLINGKNN" }, "paralog": false, "identity": 0.9967213114754099, "score": 608.0, "evalue": 1.7e-223, "description": "Internal stop codon at 75830. Nonsense mutation around 75830.", "psc": { "uniref90_id": "UniRef90_P75055", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P75055", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75055", "EC:2.3.1.234", "UniRef:UniRef50_P75055", "COG:COG0533", "COG:J", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ] }, "pscc": { "uniref50_id": "UniRef50_P75055", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75055" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "CNOCLNGIAL_175", "locus": "GCA000143945_00135" }, { "type": "cds", "sequence": "contig_1", "start": 75854, "stop": 76177, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGTCACAGTGTTTTACCAAGATTAAACAGTTAAGGGAAAGGAAAGGCTTTAATCCCCAAACCCACGATTGGAATGAGTTTGCTTCTAACTTCCAGGCCACCATTATTGATCACTACATCAATCATGTCAAGGATGCCATTCAGCAACACCAACCGCAAATGTTGCTGTTAGGTGGTGGGGTAAGTGCTAATAAATACTTAAGAGAACAGGTAACACAGTTACAGCTGCCTTACTTAATTGCGCCGCTCAAGTACACGAGCGATAACGGGGCGATGATTGGTTTTTATGCTAATCTTTTAATTAATGGCAAAAACAATTAA", "aa": "MKSQCFTKIKQLRERKGFNPQTHDWNEFASNFQATIIDHYINHVKDAIQQHQPQMLLLGGGVSANKYLREQVTQLQLPYLIAPLKYTSDNGAMIGFYANLLINGKNN", "aa_hexdigest": "3504ff01fa778645fb7059f8541d679e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12279.903899999987, "isoelectric_point": 9.335940742492678 }, "id": "CNOCLNGIAL_176", "locus": "GCA000143945_00136" }, { "type": "cds", "sequence": "contig_1", "start": 76161, "stop": 76391, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00438.25" ], "nt": "ATGGCAAAAACAATTAAACATCCCCGATGGGGTAGATATGTAGCAGAAGCAGTTGGTCGTGGTCACCCCGATAAGATCTGTGACCAGATTGCGGACAGTATCTTAGATGAATGCATTAAGCAAAGTCCGACCAGTCACGTTGCTTGTGAGGTCTTTGCTTCCAAAAATCTGATTATGGTGGGCGGGGAAATCCTTACCACTGGTTATGTTGATGTGGTCCAAACCGGTTGA", "aa": "MAKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMVGGEILTTGYVDVVQTG", "aa_hexdigest": "5e3f238bd5d55ef4d35541e6737fd9b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00438.25", "name": "S-AdoMet_synt_N", "length": 60, "aa_cov": 0.7894736842105263, "hmm_cov": 1.0, "evalue": 8.886243783987472e-25, "score": 82.66057586669922, "start": 14, "stop": 73 } ], "seq_stats": { "molecular_weight": 8256.410599999996, "isoelectric_point": 6.243501853942871 }, "id": "CNOCLNGIAL_177", "locus": "GCA000143945_00137" }, { "type": "cds", "sequence": "contig_1", "start": 76407, "stop": 77021, "strand": "+", "frame": 3, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "COG:COG0192", "COG:H", "EC:2.5.1.6", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005737", "GO:0006556", "GO:0006730", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_P78003", "UniRef:UniRef90_P78003" ], "nt": "TTGGGTTACACCGAAAATGACTTTAGCTTTTTGAGTTGTATTAACTCCCAGTCCAGTGAAATTAACCAAGCTGTGCAAAGTAATGACGAAATAGGCGCGGGCGACCAGGGCATTACCGTTGGTTATGCTTGTAGTGAAACCGAGCAATTAATGCCCTTAGGGAGTATCGTAGCGCAAGCGCTGGTGCAACGCGCTGCTCGTATCATTGACCAATACCCCTTCATTAAACACGACATGAAGAGTCAGGTAGTGCTCAACTATACTGGTAACAAAGTTCAATGTGAATCAGTTTTAATGTCGGTACAACATACTCAGGACGTAAGTTTAGACCAACTGCGCCAAACAATTATCAACCAAGTGATCCTCCCCGTATTAACGGAGTACGGTTTAAATGATCCTAAAATCAAACACCTAGTCAATCCCGGGGGTTCGTTTGTCGTTGGGGGCCCGATGGCCGATACGGGGTTAACCGGACGCAAGATTATTGTGGACACCTATGGTCCGTACGCTAATCATGGTGGGGGGAGTTTTAGTGGTAAAGACCCTACCAAGGTCGACCGCACTGGAGCTTACTTTGCGCGCTTTATTGCTAAACACATTGTTAGTTTGGGTTGA", "aa": "MGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGITVGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHDMKSQVVLNYTGNKVQCESVLMSVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTGRKIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLG", "aa_hexdigest": "546d2582b694afbac1d70544e267a248", "start_type": "TTG", "rbs_motif": null, "genes": [ "metK" ], "truncated": "both", "pseudogene": { "start": 76161, "stop": 77312, "observations": { "mutation": [ 76389, 77019, 77157, 77256 ], "stop": [ 76389, 77019, 77157, 77256 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78003", "query_cov": 1.0, "subject_cov": 0.5326370757180157, "identity": 0.995, "score": 405.0, "evalue": 1.92e-140, "gene_start": 1, "gene_end": 204, "reference_start": 83, "reference_end": 286, "reference_sequence": "MAKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMVGGEILTTGYVDVVQTGWKVLNRLGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGITVGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHDMKSQVVLNYTGNKVQCESVLMSVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTGRKIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLGWAEECEVSISWVFSQPLPQSIQVKCFNINKEFSEQLINQVISQYFNWSVAKIIAKLKLLDQVEYFRYAVYGHFGHQTAPWEQLSERDSLQCLIKNFQ" }, "paralog": false, "identity": 0.9895561357702349, "score": 755.0, "evalue": 8.28e-281, "description": "Internal stop codon at 76389, 77019, 77157, 77256. Nonsense mutation around 76389, 77019, 77157, 77256.", "psc": { "uniref90_id": "UniRef90_P78003", "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_P78003", "cog_id": "COG0192", "cog_category": "H", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005737", "GO:0006556", "GO:0006730" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78003", "EC:2.5.1.6", "UniRef:UniRef50_P78003", "COG:COG0192", "COG:H", "KEGG:K00789", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005737", "GO:0006556", "GO:0006730" ] }, "pscc": { "uniref50_id": "UniRef50_P78003", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78003" ], "product": "S-adenosylmethionine synthase" } }, "id": "CNOCLNGIAL_178", "locus": "GCA000143945_00138" }, { "type": "cds", "sequence": "contig_1", "start": 77288, "stop": 77449, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XDC4", "UniRef:UniRef90_A0AAN4XDC4" ], "nt": "ATGTTTAATCAAAAATTTCCAATAAAAGCTCCTAAAGCACCATTTTTTAAAAAGAAAACACCACCAGTACCACCAAAGAATCCTGTGGTAACCAGCACTACCCCAGAACTCAAGAAAACCAAAATTAAAAAAGTAAAGGAAGCAGGGGATACCAATCTTTGA", "aa": "MFNQKFPIKAPKAPFFKKKTPPVPPKNPVVTSTTPELKKTKIKKVKEAGDTNL", "aa_hexdigest": "770a43b7562971f7b4b06ca4e25595b0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 77288, "stop": 77569, "observations": { "mutation": [ 77447, 77534 ], "stop": [ 77447, 77534 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN4XDC4", "query_cov": 1.0, "subject_cov": 0.5698924731182796, "identity": 1.0, "score": 102.0, "evalue": 2.44e-27, "gene_start": 1, "gene_end": 53, "reference_start": 1, "reference_end": 53, "reference_sequence": "MFNQKFPIKAPKAPFFKKKTPPVPPKNPVVTSTTPELKKTKIKKVKEAGDTNLWDKIKAKVKKHPYLFAVLVSFILLIALLGWLFAIPISQIP" }, "paralog": false, "identity": 0.9247311827956989, "score": 166.0, "evalue": 8.07e-55, "description": "Internal stop codon at 77447, 77534. Nonsense mutation around 77447, 77534.", "psc": { "uniref90_id": "UniRef90_A0AAN4XDC4", "uniref50_id": "UniRef50_A0AAN4XDC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN4XDC4", "UniRef:UniRef50_A0AAN4XDC4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAN4XDC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XDC4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5935.075700000002, "isoelectric_point": 10.352284812927245 }, "id": "CNOCLNGIAL_179", "locus": "GCA000143945_00139" }, { "type": "cds", "sequence": "contig_1", "start": 77600, "stop": 77818, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGACGCGTGAAGAACGCCGGCACCCCAAATTAATTAATCGTGACCCATCGCGAAAACAACGCATCATTAAGGGTTCGGGACGTAAAATGGATGAACTCAATAAGCTAATGAAGGAGTGGAGTAAACTGCAAACGAAAACAGCAGAAATGGGCCGGATGCTGAAAGGTGGTAAAAATCCCTTTAGTGGCTTTGGTGGACTTGGTGGCTTAGGTTTTTAA", "aa": "MTREERRHPKLINRDPSRKQRIIKGSGRKMDELNKLMKEWSKLQTKTAEMGRMLKGGKNPFSGFGGLGGLGF", "aa_hexdigest": "f4b5c3aba86d1e43626ba913840e938b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 45, "aa_cov": 0.625, "hmm_cov": 0.9555555555555556, "evalue": 1.1339498617826663e-12, "score": 43.64171600341797, "start": 1, "stop": 45 } ], "seq_stats": { "molecular_weight": 8217.570200000002, "isoelectric_point": 11.053314018249512 }, "id": "CNOCLNGIAL_180", "locus": "GCA000143945_00140" }, { "type": "cds", "sequence": "contig_1", "start": 77837, "stop": 78559, "strand": "-", "frame": 2, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501", "KEGG:K03106", "SO:0001217", "UniRef:UniRef50_P75054", "UniRef:UniRef90_P75054" ], "nt": "ATGTTAGTGGCACTAGACATCTACCGCCCCGCTGCGATTGACCAGTTAGCGACCCTAGCCGAACAAACCGAGTCTGTCTTCTTTGCCAAGGGTACCCAAGCCCCCGACCAAACCACCAAGGAGGCCGTTAAGACCTTTAAGGAAAGCGGTTGTCAAGCAATAATCTGTGACACGGCGGGACGGTTACAAACTAATCAGGAATTAATGGATGAGTTGGTAGCAATTAAAAACGAACTCCACCCCGATGAAATCCTAATGGTGGTCGATGGTTTAAGTGGCCAGGAAATTATTAATGTTGCCAAGGAGTTTCATAACCGTTTAAAACTCACCGGTTTTATTATTACCAAGCTTGACAGTGATGCCCGCGCTGGAGCGGCTTTGTCCTTAACGAGTTTGTTGGAAGTTCCGATTAAACTAATGGGGACATCAGAAAAACTCACAGGTCTAGAACAATTTCACCCAGAACGGATTGCCAGTCGGATCTTGGGTTTAGGGGATGTGATGACCTTAGTGGAAAAAGTGGAAGAAGTATTTGACAAACAGAGCTTAACCAAAACAGTTAGCAAGATGTTCTTGGGCAAGATGGACCTGGAAGATCTGTTGCTTTACATGGAACAGATGAACCAAATGGGTTCTGTTAGTAGTATTGTCAAGATGCTCCCGGGTAACTTAACGGTATCGGATGATCATGTAGAGAGCATTGAAGCCAAGGTTAAACTGTGA", "aa": "MLVALDIYRPAAIDQLATLAEQTESVFFAKGTQAPDQTTKEAVKTFKESGCQAIICDTAGRLQTNQELMDELVAIKNELHPDEILMVVDGLSGQEIINVAKEFHNRLKLTGFIITKLDSDARAGAALSLTSLLEVPIKLMGTSEKLTGLEQFHPERIASRILGLGDVMTLVEKVEEVFDKQSLTKTVSKMFLGKMDLEDLLLYMEQMNQMGSVSSIVKMLPGNLTVSDDHVESIEAKVKL", "aa_hexdigest": "19be4caa2a94c2bdb055ad2629b6b0c1", "start_type": "ATG", "rbs_motif": null, "genes": [ "ffh" ], "truncated": "both", "pseudogene": { "start": 77627, "stop": 78859, "observations": { "mutation": [ 77839, 78589, 78733 ], "stop": [ 77839, 78589, 78733 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75054", "query_cov": 1.0, "subject_cov": 0.5333333333333333, "identity": 0.996, "score": 453.0, "evalue": 1.64e-157, "gene_start": 1, "gene_end": 240, "reference_start": 132, "reference_end": 371, "reference_sequence": "MFKSMISSIVMRSMQKKINAQTISETDVQAVLKEIRIALLDADVNLLVVKNFIKAIREQTVGQTVEPGQDLQKWLLKVIKQELINILSQPNQEITSKRPLKVMVVGLQGSGKTTTCGKLAVWLKKQFQQKAMLVALDIYRPAAIDQLATLAEQTESVFFAKGTQAPDQTTKEAVKTFKESGCQAIICDTAGRLQTNQELMDELVAIKNELHPDEILMVVDGLSGQEIINVAKEFHNRLKLTGFIITKLDSDARAGAALSLTSLLEVPIKLMGTSEKLTGLEQFHPERIASRILGLGDVMTLVEKAEEVFDKQSLTKTVSKMFLGKMDLEDLLLYMEQMNQMGSVSSIVKMLPGNLTVSDDHVESIEAKVKLWKVLINSMTREERRHPKLINRDPSRKQRIIKGSGRKMDELNKLMKEWSKLQTKTAEMGRMLKGGKNPFSGFGGLGGLGF" }, "paralog": false, "identity": 0.9902439024390244, "score": 773.0, "evalue": 4.26e-286, "description": "Internal stop codon at 77839, 78589, 78733. Nonsense mutation around 77839, 78589, 78733.", "psc": { "uniref90_id": "UniRef90_P75054", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P75054", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75054", "EC:3.6.5.4", "UniRef:UniRef50_P75054", "COG:COG0541", "COG:U", "KEGG:K03106", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_P75054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75054" ], "product": "Signal recognition particle protein" } }, "id": "CNOCLNGIAL_181", "locus": "GCA000143945_00141" }, { "type": "cds", "sequence": "contig_1", "start": 78731, "stop": 78952, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02881.24" ], "nt": "ATGTTTAAGTCAATGATCTCGAGCATCGTTATGCGCTCGATGCAAAAAAAGATCAACGCGCAAACGATCAGTGAAACCGATGTCCAAGCCGTTTTAAAGGAAATTCGGATTGCTCTTTTAGACGCTGACGTGAACCTGTTGGTGGTCAAAAACTTCATTAAGGCGATTCGCGAACAAACCGTTGGTCAAACCGTAGAACCCGGTCAGGACTTACAGAAGTGA", "aa": "MFKSMISSIVMRSMQKKINAQTISETDVQAVLKEIRIALLDADVNLLVVKNFIKAIREQTVGQTVEPGQDLQK", "aa_hexdigest": "bbb26c70de1b627167fec426a9fd998a", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02881.24", "name": "SRP54_N", "length": 60, "aa_cov": 0.7945205479452054, "hmm_cov": 1.0, "evalue": 1.0471247037480169e-15, "score": 53.18986892700195, "start": 16, "stop": 73 } ], "seq_stats": { "molecular_weight": 8202.591699999997, "isoelectric_point": 9.222411918640137 }, "id": "CNOCLNGIAL_182", "locus": "GCA000143945_00142" }, { "type": "cds", "sequence": "contig_1", "start": 79008, "stop": 79724, "strand": "+", "frame": 3, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152", "KEGG:K03784", "RefSeq:WP_014325316.1", "SO:0001217", "UniParc:UPI0001DD6496", "UniRef:UniRef100_A0AAV5N7K0", "UniRef:UniRef50_P47295", "UniRef:UniRef90_P75053" ], "nt": "ATGACACCGCACATAAACGCAAAAAAAGATGACATTGCTAAGGTTGTTTTAATGCCCGGTGATCCCTTACGTGCTAAGTGGATTGCGGAACAATTTATGGAAAAGCCCCGGCTCGTCAACGAAGTGCGGGGCATGTTAGCTTTTACCGGTCAGTATAAGGGTAAGACCATTACCATTATGGGCCATGGCATGGGGATTCCCTCAATTGGGATTTATTCTTATGAACTGATGAAGTTCTATGAGGTTAATACCATTATCCGAATTGGTAGTTGCGGGGCGTTGCAAGATTCCTTAAACCTCCAGGACCTCATTATTGCCGCTAAGGCTTGGAGTGAGTCGATTTACGCTAACGACATGGGTGTGGAAGTGCCAGCAGACAAGATCTTAATGGCCTCCCCGCAACTAGTAGAGTTAGCTAAAAAGACCGCTAACCAACTTCAACTGGCCTTCCATGAGGGCTTGGTGTTCTGTGAGGATGCCTTCCACCAAACCAGGAAAGACGTGTTAAAGCTAGCACAAGAAAAACACGCTTTAGCAGTGGAAATGGAAGCGCATGCTCTGTACGCTAATGCCATGCTGCTCAACAAGCAAGCGTTAACGATGCTCACGGTGTCCGATTCTTTGGTAACCCATGCTGCTCTACCAGCGGAACAACGCCAAGCCACCTTTAAGAACATGGCTATCTTATCCTTGGAAATGGCTAGCCAACTCGTTTAA", "aa": "MTPHINAKKDDIAKVVLMPGDPLRAKWIAEQFMEKPRLVNEVRGMLAFTGQYKGKTITIMGHGMGIPSIGIYSYELMKFYEVNTIIRIGSCGALQDSLNLQDLIIAAKAWSESIYANDMGVEVPADKILMASPQLVELAKKTANQLQLAFHEGLVFCEDAFHQTRKDVLKLAQEKHALAVEMEAHALYANAMLLNKQALTMLTVSDSLVTHAALPAEQRQATFKNMAILSLEMASQLV", "aa_hexdigest": "8ca92d861fe0e9828a7e17179010bc50", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6496", "ncbi_nrp_id": "WP_014325316.1", "uniref100_id": "UniRef100_A0AAV5N7K0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6496", "RefSeq:WP_014325316.1", "UniRef:UniRef100_A0AAV5N7K0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N7K0", "uniref90_id": "UniRef90_P75053" }, "psc": { "uniref90_id": "UniRef90_P75053", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_P47295", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152" ] }, "pscc": { "uniref50_id": "UniRef50_P47295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47295" ], "product": "Purine nucleoside phosphorylase DeoD-type" }, "genes": [ "deoD" ], "id": "CNOCLNGIAL_183", "locus": "GCA000143945_00143" }, { "type": "cds", "sequence": "contig_1", "start": 79728, "stop": 80402, "strand": "+", "frame": 3, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_010874420.1", "SO:0001217", "UniParc:UPI00001291AA", "UniRef:UniRef100_P09924", "UniRef:UniRef50_P09924", "UniRef:UniRef90_P09924" ], "nt": "ATGAAATTAGAATACAACCGCATTATTGACAGTACACTACTTAAAGCTGATACCTTACCCCACGAAATTGATGCTTTATGTGCTGATGCTCATAAGTACCAGTTTTACGCAGTTTGTGTCAATCCTAGCTATGTGCGGTATGCCAAAAATATCCTTAAGGGTACGGGGGTGAAACTGTGCACTGTCGTTGGTTTTCCCTTAGGACAAACAACACAACGCCAGAAGGTGTATGAAACTAAGATTGCCATTAAGGAAGGTGCGGATGAAATTGACATGGTAATGAACATTGCCGAGTTCAAAAAGCGCTGTGCCTGTGTCATTAGTGAAATAAGGGCAGTCAAAAAGGTTTGTGGTAAGCGAACGCTCAAAGTCATTATTGAAACGGCTTTGTTAAACCAGGACGAAATTCGTGATGCCGTTAATGTCTGTATTGATGGTAACGCTGACTTTGTCAAAACTTCTACCGGGTTTTCCATGCGTGGTGCATCATTGGAAGATATCACAATTATGCGCGAAGCTAGTGGTAATCTCATTAAGATCAAAGCATCGGGTGGGGTTCAAACAGCGCAGCAATTCCTTGACTTCTTTAATGCTGGAGTTAGTCGGATTGGCACTTCCAATGCGGTCAAGATTATGGAAGAATTGCACAAACTAGAAAGCCATGAACATCGTTAA", "aa": "MKLEYNRIIDSTLLKADTLPHEIDALCADAHKYQFYAVCVNPSYVRYAKNILKGTGVKLCTVVGFPLGQTTQRQKVYETKIAIKEGADEIDMVMNIAEFKKRCACVISEIRAVKKVCGKRTLKVIIETALLNQDEIRDAVNVCIDGNADFVKTSTGFSMRGASLEDITIMREASGNLIKIKASGGVQTAQQFLDFFNAGVSRIGTSNAVKIMEELHKLESHEHR", "aa_hexdigest": "b1a5656f05421d9203c65b191539935d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001291AA", "ncbi_nrp_id": "WP_010874420.1", "uniref100_id": "UniRef100_P09924", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001291AA", "RefSeq:WP_010874420.1", "UniRef:UniRef100_P09924" ] }, "ips": { "uniref100_id": "UniRef100_P09924", "uniref90_id": "UniRef90_P09924" }, "psc": { "uniref90_id": "UniRef90_P09924", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_P09924", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_P09924", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P09924" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "CNOCLNGIAL_184", "locus": "GCA000143945_00144" }, { "type": "cds", "sequence": "contig_1", "start": 80389, "stop": 80526, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02885.22" ], "nt": "ATGAACATCGTTAACCTTATTAGTAAAAAGCAACGCGGCAAGGCGTTAACGGAAACAGAAATTAACTGGTTTGTCCATTCCGTTAATAACAAAAGCTTGGCCGATTATCAGGTCAGTGCCTTTTTAATGGCAGTGTGA", "aa": "MNIVNLISKKQRGKALTETEINWFVHSVNNKSLADYQVSAFLMAV", "aa_hexdigest": "2ba27ac4f98986486a333c854ad6f3ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02885.22", "name": "Glycos_trans_3N", "length": 41, "aa_cov": 0.9111111111111111, "hmm_cov": 1.0, "evalue": 3.505338530920689e-12, "score": 41.433345794677734, "start": 5, "stop": 45 } ], "seq_stats": { "molecular_weight": 5109.8771000000015, "isoelectric_point": 9.398990821838382 }, "id": "CNOCLNGIAL_185", "locus": "GCA000143945_00145" }, { "type": "cds", "sequence": "contig_1", "start": 80536, "stop": 81177, "strand": "+", "frame": 1, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "nt": "ATGAATTCCAAGGAGTTGTTTTGTCTCACCAAAGCGATGGTGAAATCAGGAGAGAGCCTCCACTTTAACCACCACAGCAAGTTAAGTGTTGACAAACACTCCACTGGTGGTATTGGTGACAAGGTTTCCATTGCTTTAATTCCAATCTTAACTGCCTTAGATTACAGTGTCGCAAAACTGTCGGGTCGTGGTTTGGGCTACACTGGTGGCACAATTGACAAATTGGAAGCAGTGGGAGTAAAAACAGATTTTACACCAACAGAGGCGCAGAACTTACTAGACCAAAATGACTGCTTTATTATTGGTCAAAGTGAGGGCATTGCTCCCGTAGACAAGGTGTTGTATGCACTACGCGATACTACGGCAACTGTGGACAGTCTGCCCTTAATTGCTTCCAGTGTCATGTCGAAGAAGTTGGCAATTAACAACGACTACATCTTCATTGACCTTAAGTACGGTAAGGGCGCTTTCTGTAAAACGAAGACGATGGCTAAAGAGCTAGCGCAGTACATGTACAGTATTGCCAAGCAGTTTAAGCGCAAACTCTACATTAAACTAAGTGACATGAACCAGGTTTTGGGTAAAACGATTGGTAATGCGTTAGAGGTCTTGGAAGTAGTGCACTTCTTAAAACGGAATTGA", "aa": "MNSKELFCLTKAMVKSGESLHFNHHSKLSVDKHSTGGIGDKVSIALIPILTALDYSVAKLSGRGLGYTGGTIDKLEAVGVKTDFTPTEAQNLLDQNDCFIIGQSEGIAPVDKVLYALRDTTATVDSLPLIASSVMSKKLAINNDYIFIDLKYGKGAFCKTKTMAKELAQYMYSIAKQFKRKLYIKLSDMNQVLGKTIGNALEVLEVVHFLKRN", "aa_hexdigest": "655979a94d3c0d66a21478ef55532664", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A66C754", "query_cov": 0.9295774647887324, "subject_cov": 0.8016194331983806, "identity": 0.5, "score": 185.0, "evalue": 2.75e-55, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A354QR12", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A354QR12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "CNOCLNGIAL_186", "locus": "GCA000143945_00146" }, { "type": "cds", "sequence": "contig_1", "start": 81184, "stop": 81654, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "GTGGGTGCTGACTTTATTCAGTTGATGGAGCAAATAGTCACTGAAATCCTTATAGAAACAAAACGTGCACCAAACAAACGGGCAGCTGTAGCACTTTACCACGCTACATTAGAGGGCGAAAAACCATGGCAGCGTTTTTTAAAATTTATTGAGCTGCAAGGTAGTAGTCGGGAACGTTTTTTGGATTTAAAAGAGCTGTTTAATCCCCAGTATAAAGCGCCAGTGTTAGCATCTCAAAGTGGTACTTTGAGCTATACCAGTCCGGTGGATTTAGCCATGGTCTCGATTAGCTTAGGTGCTGGTCGAATGGTTAAAACAGATCTGATTGACCCAATGGCGGGGATTAAGTTAGTGAAACAAGCTAACGAAGTGGTAAAAGCTGGGGACACAGTCTTAGAACTTTACAGCTCCAAACCAATTACGCCTGCCCACATAGAAGCTGCGCAGCACACCATTATTATTAAGCAGTAA", "aa": "MGADFIQLMEQIVTEILIETKRAPNKRAAVALYHATLEGEKPWQRFLKFIELQGSSRERFLDLKELFNPQYKAPVLASQSGTLSYTSPVDLAMVSISLGAGRMVKTDLIDPMAGIKLVKQANEVVKAGDTVLELYSSKPITPAHIEAAQHTIIIKQ", "aa_hexdigest": "c9b5b2c9513f642a177bfe52f3babe3a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 73, "aa_cov": 0.46153846153846156, "hmm_cov": 1.0, "evalue": 5.562358219349716e-22, "score": 72.7433090209961, "start": 83, "stop": 154 } ], "seq_stats": { "molecular_weight": 17278.940199999994, "isoelectric_point": 7.942005348205567 }, "id": "CNOCLNGIAL_187", "locus": "GCA000143945_00147" }, { "type": "cds", "sequence": "contig_1", "start": 81707, "stop": 82066, "strand": "+", "frame": 2, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802", "KEGG:K01489", "SO:0001217", "UniRef:UniRef50_P75051", "UniRef:UniRef90_P75051" ], "nt": "GTGGTCAATCATGCCTACACGCCTTTTTCAAAGTTTAAGGTAGCTTGTATGTTGGTGGCCAATAACCAGGCATTTTATGGAGTCAATATTGAAAACGCTTCTTACCCAGTAACCCTGTGTGCGGAACGTAGTGCGATTGCTAACATGGTCACTAGTATCGGCAAGGCCACAATTGATTATGTCTTTGTGTACTTTGACACTAAAACTCCAACCAATTCACCTTGTGGCATGTGTCGGCAAAACATCTTTGAGTTTGCCACTGATAAGACCCAGTTATTCTGCATTGAGAAGGACAAGAGCTTTAAACAATTTACTATTCCCGAAATTTTAAAGGGAGGTTTTCGATCATATGAACAGTAA", "aa": "MVNHAYTPFSKFKVACMLVANNQAFYGVNIENASYPVTLCAERSAIANMVTSIGKATIDYVFVYFDTKTPTNSPCGMCRQNIFEFATDKTQLFCIEKDKSFKQFTIPEILKGGFRSYEQ", "aa_hexdigest": "5c45effc65b270d0ef05793c0fa32ae9", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75051", "query_cov": 1.0, "subject_cov": 0.8947368421052632, "identity": 0.992, "score": 246.0, "evalue": 1.24e-82, "valid": true, "gene": "cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_P75051", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802" ] }, "pscc": { "uniref50_id": "UniRef50_P75051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75051" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "CNOCLNGIAL_188", "locus": "GCA000143945_00148" }, { "type": "cds", "sequence": "contig_1", "start": 82056, "stop": 82901, "strand": "+", "frame": 3, "gene": "manB", "product": "Phosphomannomutase", "db_xrefs": [ "COG:COG1109", "COG:G", "EC:5.4.2.8", "GO:0000287", "GO:0004615", "GO:0005975", "GO:0006166", "GO:0008973", "SO:0001217", "UniRef:UniRef50_P47299", "UniRef:UniRef90_P75050" ], "nt": "ATGAACAGTAACGCATACTTGGAAGCTCAAAGGTGGCTAAGCCATCCGCGGGTAAAACCCAACTTAAAGGAGGTCATTACGGCTATGAGTGCTGAGGAAATCGAGCACTTCTTTAGCTTAAAGAAACCGAGTTTTGGCACTGCTGGGGTACGGGGCAAAATGGCACCGGGGTACCATGGGATGAATGTGTTTTCCTATGCTTACTTAACCCAGGGTTATGTCAACTACATCCAGTCGTTAAACCCAACTAAAAAACCATTACGCTTTTTAGTAGCCAGAGACACTAGAAAACACGGGGCATTGTTTAATGGCATTGTGTGTGATGTGATTACCTCCATGGGTCATGTGGTCTATATGTTTGATAACAATGAACCAACACCAACACCATTAGTATCTTATGTGATTAAGAAGTACCACTTTGATGGTGGGGTGAATGTCACTGCTAGTCATAATCCTAAAACCGATAATGGCTTTAAGATTTACGATGGTCATGGCGCGCAGTTACTAGACTTCCAAACTGATCAATTAATCGCAATGTTACCCCCAGTGGTAACAATGTTAGATTTCGAACCACGTGGTAACAACGAGCTGTTACACTTTTTGGATAACGAAGTGGTCTACAAAAACTACTTTGATGATCTTAAGGAAAGCCTAGTAGTGGACAACGACAGCTTTAAAAACCTCCCGGTGGTCTTTACCGGGTTACATGGTACTTCGGTGAAGTTGTTACCAAGGTTTTTAACCTATTTGGGTTACAGCAACATTATTAGTGTCCAACCACAAAACGTGTTTGACGCTAACTTTGCTAATGCTGACCACCTCAACCCCGAAAGTAAGGATACCTGA", "aa": "MNSNAYLEAQRWLSHPRVKPNLKEVITAMSAEEIEHFFSLKKPSFGTAGVRGKMAPGYHGMNVFSYAYLTQGYVNYIQSLNPTKKPLRFLVARDTRKHGALFNGIVCDVITSMGHVVYMFDNNEPTPTPLVSYVIKKYHFDGGVNVTASHNPKTDNGFKIYDGHGAQLLDFQTDQLIAMLPPVVTMLDFEPRGNNELLHFLDNEVVYKNYFDDLKESLVVDNDSFKNLPVVFTGLHGTSVKLLPRFLTYLGYSNIISVQPQNVFDANFANADHLNPESKDT", "aa_hexdigest": "4dd14b0aa519f837ae19bfa0fa1c675f", "start_type": "ATG", "rbs_motif": null, "genes": [ "manB" ], "truncated": "3-prime", "pseudogene": { "start": 82056, "stop": 83204, "observations": { "mutation": [ 82899, 82989 ], "stop": [ 82899, 82989 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75050", "query_cov": 1.0, "subject_cov": 0.5072202166064982, "identity": 1.0, "score": 576.0, "evalue": 1.02e-203, "gene_start": 1, "gene_end": 281, "reference_start": 1, "reference_end": 281, "reference_sequence": "MNSNAYLEAQRWLSHPRVKPNLKEVITAMSAEEIEHFFSLKKPSFGTAGVRGKMAPGYHGMNVFSYAYLTQGYVNYIQSLNPTKKPLRFLVARDTRKHGALFNGIVCDVITSMGHVVYMFDNNEPTPTPLVSYVIKKYHFDGGVNVTASHNPKTDNGFKIYDGHGAQLLDFQTDQLIAMLPPVVTMLDFEPRGNNELLHFLDNEVVYKNYFDDLKESLVVDNDSFKNLPVVFTGLHGTSVKLLPRFLTYLGYSNIISVQPQNVFDANFANADHLNPESKDTWELARQYASNTKAKLMMAIDPDADRFAIAEWNPQTQDWHYFSGNESGVMVAYYKLKHKQFKRQPYIVTTVVSTDLVDKIAKKYGAFVKRTNVGFKFIGQAVNHFSKDNELVVAFEEAIGMMASDGLNREKDSFQAAAIMLEIARYCHNKGISLLEFYRGEIFGEFGDYYNWTVPHTIHGVNWKEKMEQVLHQLTTATIKEVVGHKITKIKNYVDINLVEYVLENGNWIKFRISGTEPKLKLYFNLSNGYLAALKHEAKKMHEFLVRLLNLDKA" }, "paralog": false, "identity": 0.9947643979057592, "score": 774.0, "evalue": 5.08e-284, "description": "Internal stop codon at 82899, 82989. Nonsense mutation around 82899, 82989.", "psc": { "uniref90_id": "UniRef90_P75050", "gene": "manB", "product": "Phosphomannomutase", "ec_ids": [ "5.4.2.8" ], "uniref50_id": "UniRef50_P47299", "cog_id": "COG1109", "cog_category": "G", "go_ids": [ "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75050", "EC:5.4.2.8", "UniRef:UniRef50_P47299", "COG:COG1109", "COG:G", "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_P47299", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47299" ], "product": "Phosphomannomutase" } }, "id": "CNOCLNGIAL_189", "locus": "GCA000143945_00149" }, { "type": "cds", "sequence": "contig_1", "start": 83043, "stop": 83411, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "ATGGTGGCTTACTATAAGCTCAAACACAAGCAGTTTAAGCGTCAACCTTACATTGTAACGACGGTAGTCTCTACTGATCTAGTGGATAAGATTGCCAAGAAGTACGGGGCTTTTGTCAAGCGCACAAATGTCGGCTTTAAGTTTATTGGTCAAGCTGTTGATCACTTCTCCAAAGACAATGAACTAGTAGTGGCCTTTGAAGAAGCGATTGGTATGATGGCTAGTGATGGTTTGAACCGCGAAAAGGATTCCTTTCAAGCAGCGGCCATCATGCTTGAAATTGCGCGCTACTGTCATAATAAAGGAATTAGCTTACTAGAGTTCTACCGTGGTGAGATCTTCGGTGAATTTGGTGATTACTATAACTGA", "aa": "MVAYYKLKHKQFKRQPYIVTTVVSTDLVDKIAKKYGAFVKRTNVGFKFIGQAVDHFSKDNELVVAFEEAIGMMASDGLNREKDSFQAAAIMLEIARYCHNKGISLLEFYRGEIFGEFGDYYN", "aa_hexdigest": "6ee8b8e379ef7ade73fb9f010b5881fb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 102, "aa_cov": 0.8360655737704918, "hmm_cov": 0.9215686274509803, "evalue": 2.9746189770401576e-16, "score": 55.0925407409668, "start": 3, "stop": 104 } ], "seq_stats": { "molecular_weight": 13995.943299999992, "isoelectric_point": 8.59100856781006 }, "id": "CNOCLNGIAL_190", "locus": "GCA000143945_00150" }, { "type": "cds", "sequence": "contig_1", "start": 83454, "stop": 83720, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00408.26" ], "nt": "ATGGAACAGGTATTACACCAGTTAACCACAGCTACCATTAAAGAGGTTGTGGGACACAAGATTACCAAGATTAAAAACTATGTCGACATTAACCTAGTCGAATATGTCTTGGAAAACGGTAACTGGATTAAGTTCCGCATTTCCGGTACAGAACCGAAGTTAAAGCTGTACTTTAACCTATCTAAAGGTTACTTAGCAGCTTTAAAACACGAAGCGAAAAAGATGCACGAGTTCCTAGTGCGATTGCTCAACCTCGATAAAGCTTAG", "aa": "MEQVLHQLTTATIKEVVGHKITKIKNYVDINLVEYVLENGNWIKFRISGTEPKLKLYFNLSKGYLAALKHEAKKMHEFLVRLLNLDKA", "aa_hexdigest": "bdee44ac741da2c61bdd4ad82e7d05d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00408.26", "name": "PGM_PMM_IV", "length": 34, "aa_cov": 0.38636363636363635, "hmm_cov": 0.9117647058823529, "evalue": 9.859676873438439e-07, "score": 24.284587860107422, "start": 26, "stop": 59 } ], "seq_stats": { "molecular_weight": 10238.96979999999, "isoelectric_point": 9.522512245178223 }, "id": "CNOCLNGIAL_191", "locus": "GCA000143945_00151" }, { "type": "cds", "sequence": "contig_1", "start": 83688, "stop": 83942, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAATTCCTTACTTGGGTAAATACGAAATTGTCGAAGGTGACACTAAGGTCGATCAACTGCAACAATTTAGTGTTGGTAACTTAGTGGAAGTCCACTTAACTGGTGCGATTCACATTCAAGGTCAAATTAAGGCTCTATACCAAGGAACAATTAACAAAGCGGTAGTGGAAGTCGAATTAACCACCAAAACGCAGTTAATTAACTTGCCTTTAGAAAACCTAAGCTTTATCGAGGTTGAGCAATCGCACTAG", "aa": "MLIPYLGKYEIVEGDTKVDQLQQFSVGNLVEVHLTGAIHIQGQIKALYQGTINKAVVEVELTTKTQLINLPLENLSFIEVEQSH", "aa_hexdigest": "27954ce4ce3ea4788752e13f3c5fb45b", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9389.689299999996, "isoelectric_point": 4.931999015808104 }, "id": "CNOCLNGIAL_192", "locus": "GCA000143945_00152" }, { "type": "cds", "sequence": "contig_1", "start": 83994, "stop": 84221, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAGGGTATTTCGGTCAATCCGAAAAAGCGGGTGTTAGTGACCAACACTGCCATTGTGGAAATGGATGCAGACAAGTTTGACGAAAAGTTTCAGTACATTCTCAAGCACAAACAAGTTAAACCCGAAGCAATTGCCCAAGTTAACGAATCTGGTGAAATCATTGATACTAACCAGTTTGCGCAAGCTCTAATGGAAGCTAATAAGGCCGAACAAGACGAATGA", "aa": "MLKGISVNPKKRVLVTNTAIVEMDADKFDEKFQYILKHKQVKPEAIAQVNESGEIIDTNQFAQALMEANKAEQDE", "aa_hexdigest": "a5de885eef23d32d9bdd17caf7f8484f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8491.595299999999, "isoelectric_point": 5.112064933776855 }, "id": "CNOCLNGIAL_193", "locus": "GCA000143945_00153" }, { "type": "cds", "sequence": "contig_1", "start": 84327, "stop": 84425, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGATGAAGACCGTTATTTAAAGGTGAAGATTAGCGAGAAGAATTTACTAGGTCGTTACATCTACATTAAGATGATCTACAGTGAAGATGCATGA", "aa": "MLDEDRYLKVKISEKNLLGRYIYIKMIYSEDA", "aa_hexdigest": "b3662008d42eb2cf2cc38dec11d7d1b6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3911.5431000000017, "isoelectric_point": 6.060196113586425 }, "id": "CNOCLNGIAL_194", "locus": "GCA000143945_00154" }, { "type": "cds", "sequence": "contig_1", "start": 84429, "stop": 84584, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGAAACCTCAAAGCTAAGGTAAAAGCTTTGGGATTTGACAACGAAATCTTAGATGTAAGGGTTTTGAAAGAACGCGAAGTCATCGAAGAAGTCTTTTCCTTAAAATCCGGCAAGCTGCCCCGTTCATTAAAGAACACCGCTTTTACAAAGTGA", "aa": "MRNLKAKVKALGFDNEILDVRVLKEREVIEEVFSLKSGKLPRSLKNTAFTK", "aa_hexdigest": "ee1c3213417f222481e8cba1a563eafc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5860.871099999999, "isoelectric_point": 9.991777610778808 }, "id": "CNOCLNGIAL_195", "locus": "GCA000143945_00155" }, { "type": "cds", "sequence": "contig_1", "start": 84658, "stop": 85035, "strand": "-", "frame": 3, "gene": "secE", "product": "preprotein translocase subunit SecE", "db_xrefs": [ "COG:COG0690", "COG:U", "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952", "RefSeq:WP_010874425.1", "SO:0001217", "UniParc:UPI00001391AC", "UniRef:UniRef100_P75048", "UniRef:UniRef50_P47301", "UniRef:UniRef90_P75048" ], "nt": "ATGGAAAAAAAGAAACTCCCTTTTGGTTTAAAGAACAAGGAAAAACTCACCGCCTACAACGATGAGAAGATCCACGAGTTACACCGTCAACTAAAGGCCAAAATAGAAGCCAAGAAGGCCAAAGAGAAACAAGACAGTAAAACTAAAGACACAGATAAAAAAGTGGACCAAACCCCAAAGGTTAAGGTGCCTTTTACCAAGAAGTTTTCGAACCTGTGGTTTGGGATTGACAAAGAGGTTAACAAGATTGTCTGGGTAACAAGCAAGAAATTAATTACGATCTTTTTGTTAATTGTTTTAGTTAGTGCCATTATGATAGGGATCTACTTTGGCATTAACCACCTCTTTATTGCGCTTGGAGTTTTTAAAGGTAAATAA", "aa": "MEKKKLPFGLKNKEKLTAYNDEKIHELHRQLKAKIEAKKAKEKQDSKTKDTDKKVDQTPKVKVPFTKKFSNLWFGIDKEVNKIVWVTSKKLITIFLLIVLVSAIMIGIYFGINHLFIALGVFKGK", "aa_hexdigest": "98528c5cc6aa9788f61bf33ebaabfa7a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001391AC", "ncbi_nrp_id": "WP_010874425.1", "uniref100_id": "UniRef100_P75048", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391AC", "RefSeq:WP_010874425.1", "UniRef:UniRef100_P75048" ] }, "ips": { "uniref100_id": "UniRef100_P75048", "uniref90_id": "UniRef90_P75048" }, "psc": { "uniref90_id": "UniRef90_P75048", "gene": "secE", "product": "preprotein translocase subunit SecE", "uniref50_id": "UniRef50_P47301", "cog_id": "COG0690", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_P47301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47301" ], "product": "Uncharacterized protein MG055" }, "genes": [ "secE" ], "id": "CNOCLNGIAL_196", "locus": "GCA000143945_00156" }, { "type": "cds", "sequence": "contig_1", "start": 85284, "stop": 85586, "strand": "-", "frame": 1, "gene": null, "product": "MPN070 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75047", "UniRef:UniRef90_P75047" ], "nt": "ATGAAAGTGATTAAGAAGCTCAATCTGCTCAATGAACTAACGATGCTGGTGTGCATCTTCTTCTTTGTGTGCACTATCTCCCTAATTGGCATTGGCATTATGTATGACCTCATTAACAGAACTTCATTGAGTGCACCACGTCGTGATCCGATTTTTCGGAACCTCAACACAGTCTTAATTGTTTTAGGGGTGTTAGAAATCCTTTTAATGGTGGCCCAATTAGTTATGTCCAATATGGCAGCCAACATTATTAACGAGGTTGCCGAAAACGTCGAGCAAAAGTTTGCTAAAGCCTTGAAATGA", "aa": "MKVIKKLNLLNELTMLVCIFFFVCTISLIGIGIMYDLINRTSLSAPRRDPIFRNLNTVLIVLGVLEILLMVAQLVMSNMAANIINEVAENVEQKFAKALK", "aa_hexdigest": "50d308f747f8078bac6dedf0b9c74f0e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 85203, "stop": 85586, "observations": { "mutation": [ 85286 ], "stop": [ 85286 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75047", "query_cov": 1.0, "subject_cov": 0.7874015748031497, "identity": 1.0, "score": 178.0, "evalue": 4.56e-56, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MKVIKKLNLLNELTMLVCIFFFVCTISLIGIGIMYDLINRTSLSAPRRDPIFRNLNTVLIVLGVLEILLMVAQLVMSNMAANIINEVAENVEQKFAKALKWSRFLPFGLLQLYCYHKIKLVTQTDNI" }, "paralog": false, "identity": 0.9921259842519685, "score": 243.0, "evalue": 5.8e-84, "description": "Internal stop codon at 85286. Nonsense mutation around 85286.", "psc": { "uniref90_id": "UniRef90_P75047", "product": "MPN070 family protein", "uniref50_id": "UniRef50_P75047", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75047", "UniRef:UniRef50_P75047", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75047", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75047" ], "product": "Uncharacterized protein MG055.2 homolog" } }, "id": "CNOCLNGIAL_197", "locus": "GCA000143945_00157" }, { "type": "cds", "sequence": "contig_1", "start": 85573, "stop": 86403, "strand": "-", "frame": 3, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677", "KEGG:K07056", "RefSeq:WP_014325321.1", "SO:0001217", "UniParc:UPI0001DD649B", "UniRef:UniRef100_A0AAV5N9J1", "UniRef:UniRef50_P47302", "UniRef:UniRef90_P75046" ], "nt": "ATGCCAGCTCTTAAAGTAATTGCCACCCCCATTGGCAACATTGAGGAAGTTAGTCCGCGAGTTAAAGCGGCTTTAACCAAGTGTGAAGTGCTCTTTTGTGAGGACACCAGAGTAACCAAAAAACTCTTGGGCTTACTCGGCATTGACTTTTCAAATAAACAGTTCATTATTAACAACGAGTTTAAGGAAAAGCAAAACTTAAGTAAGGTAGCTAACTTAATCCACCAGTATCACTGTGGTTTGGTCAGTGATGCGGGTTATCCCTCAGTATCCGATCCCGGGCACATATTGGTGGAGTATGTCAGAACGCAACTACCCCAAATCGCAATAGAAGTAATTAATGGTCCTAGTGCGCTGGTTTGTGGTTTGGTAACCAGTGGCTTCCCCGAAAGTCCACTACTGTTCTTGGGCTTCTTGGACCACAAACCCACTCAAATAACCCAAACACTCAAACACTACCAAAACTTCCAAGGCACCATTGTTTTATTTGAAGCGGTACACCGTTTACAACAAACACTGGAAGTAATCCAAACGGTGTTTAGTAATACTGAAGTTTTTGTAGGGCGGGAACTGACCAAGCTCCACGAAAGCCACTACTGGTTTAATACAAGTGCACCGCTGCCAGACATTACTCTAAAGGGCGAGTTTGTGATTGTGATTAACAACCACCACACCCAACCGGTGGGGCAATATTCCGACCAACTCTTAAAACAAGAAATTACCCAATTAGTCCAAATGGGCGTTAAGGTTAAGGATGCTTGTCATTATTTAGCTAAACGATTGCAATTAAAATCAAATAAGCTCTACACCTTATTCCATGAAAGTGATTAA", "aa": "MPALKVIATPIGNIEEVSPRVKAALTKCEVLFCEDTRVTKKLLGLLGIDFSNKQFIINNEFKEKQNLSKVANLIHQYHCGLVSDAGYPSVSDPGHILVEYVRTQLPQIAIEVINGPSALVCGLVTSGFPESPLLFLGFLDHKPTQITQTLKHYQNFQGTIVLFEAVHRLQQTLEVIQTVFSNTEVFVGRELTKLHESHYWFNTSAPLPDITLKGEFVIVINNHHTQPVGQYSDQLLKQEITQLVQMGVKVKDACHYLAKRLQLKSNKLYTLFHESD", "aa_hexdigest": "e7b611fc76802afab10b3fb68d0e176f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD649B", "ncbi_nrp_id": "WP_014325321.1", "uniref100_id": "UniRef100_A0AAV5N9J1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD649B", "RefSeq:WP_014325321.1", "UniRef:UniRef100_A0AAV5N9J1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9J1", "uniref90_id": "UniRef90_P75046" }, "psc": { "uniref90_id": "UniRef90_P75046", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_P47302", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677" ] }, "pscc": { "uniref50_id": "UniRef50_P47302", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47302" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [ "rsmI" ], "id": "CNOCLNGIAL_198", "locus": "GCA000143945_00158" }, { "type": "cds", "sequence": "contig_1", "start": 86385, "stop": 86921, "strand": "-", "frame": 1, "gene": "rnmV", "product": "ribonuclease M5", "db_xrefs": [ "COG:COG1658", "COG:J", "EC:3.1.26.8", "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872", "KEGG:K05985", "RefSeq:WP_010874429.1", "SO:0001217", "UniParc:UPI00001391B7", "UniRef:UniRef100_P75045", "UniRef:UniRef50_P75045", "UniRef:UniRef90_P75045" ], "nt": "ATGGATAAAAAAACACGTCTAAAACTAGATGGGGTGATAGTGTGTGAGGGCAAAACTGACCAAGCTCGCTTACAACAGCTGTTTGATGTTAGTGTTATTACCACGAACGGTTCAGCTTTAAACCAAAGAACCATTAACCTGATTAAGGCTGTAGCTAAAAAACAACCGGTGATCCTCTTCTTGGACCCCGATGTTGCTGGTCAGAAAATTCGCCGGCAGTTAGAACAACACTTAGACAAGTATGAAAGTTGTTTTATAGCTCGGAAAGACATGAAACCCAACAGCACTAAAATTGGGGTAGCGGAAGCAACTGATGCAGCTTTAATCCAAGCGTTACAACAACGTCAGGTGTTCACTAAAACAACCCAACCCACTTTAAGCTGGGAACAATATTTGGAATTAAACCTCAACAGTAAAAGTAAACGGTTAGCGTTGTGTAACAAACTACACTTAAGCTACTTTAACCACAAGCAGCTCTTTCGCAAACTTAATTTACTCCAACTTACCTTTGACCAAGTATGCCAGCTCTTAAAGTAA", "aa": "MDKKTRLKLDGVIVCEGKTDQARLQQLFDVSVITTNGSALNQRTINLIKAVAKKQPVILFLDPDVAGQKIRRQLEQHLDKYESCFIARKDMKPNSTKIGVAEATDAALIQALQQRQVFTKTTQPTLSWEQYLELNLNSKSKRLALCNKLHLSYFNHKQLFRKLNLLQLTFDQVCQLLK", "aa_hexdigest": "44a08099613c1fb03f33930446392a15", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001391B7", "ncbi_nrp_id": "WP_010874429.1", "uniref100_id": "UniRef100_P75045", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391B7", "RefSeq:WP_010874429.1", "UniRef:UniRef100_P75045" ] }, "ips": { "uniref100_id": "UniRef100_P75045", "uniref90_id": "UniRef90_P75045" }, "psc": { "uniref90_id": "UniRef90_P75045", "gene": "rnmV", "product": "ribonuclease M5", "ec_ids": [ "3.1.26.8" ], "uniref50_id": "UniRef50_P75045", "cog_id": "COG1658", "cog_category": "J", "kegg_orthology_id": "K05985", "go_ids": [ "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75045", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75045" ], "product": "Ribonuclease M5" }, "genes": [ "rnmV" ], "id": "CNOCLNGIAL_199", "locus": "GCA000143945_00159" }, { "type": "cds", "sequence": "contig_1", "start": 86900, "stop": 87886, "strand": "-", "frame": 2, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "GO:0000287", "GO:0002189", "GO:0004749", "GO:0005524", "GO:0005737", "GO:0006015", "GO:0006164", "GO:0009156", "GO:0016301", "KEGG:K00948", "RefSeq:WP_014574862.1", "SO:0001217", "UniParc:UPI0001DD649C", "UniRef:UniRef100_A0AAV5NA32", "UniRef:UniRef50_P47304", "UniRef:UniRef90_P75044" ], "nt": "ATGGATCGCCATAACCACGTTGTTTTTAGCTTATCGAAAACCCACGACTTAGTTAGTCGTATTTGTCAGAAACTCAAAATGCCCATGGGGTTAATTACCCATAATGAGTTTGCTGATGGGGAGACCTACATCCGCTTTGAGGAGTCTGTGCGCAATAAGGATGTTTTTATCTTTCAGTCTACCTGTGCGCCAGTCAACGATTCCTTAATGGAATTGTTAATAGCAATTGATGCCTTAAAACGGGGTAGTGCAAAAAGTATTACCGCCATTTTGCCGTACTATGGTTATGCACGGCAAGATCGCAAAACAATGGGACGCGAACCGATAACTAGTAAATTAGTAGCGGATTTGTTAACCACGGCTGGGGTGAGCCGCGTAGCTTTAACCGACATCCACAGCGACCAAACCCAGGGTTTCTTTAATATACCCGTGGATACATTGCAAACCTACCACGTCTTTTTAACCCGCACTGTAGAGCTGTTAGGTAAAAAAGACCTTGTGGTGGTGTCCCCCGATTATGGCGGTGTAAAGCGGGCAAGGTTAATTGCAACATCTTTGGAACTACCGCTAGCGATTATTGACAAGCGTCGCCCAGCCCACAATGTAGCGGAATCAATTAATGTCTTGGGTGAAGTAGCGAACAAAAACTGTCTAATAGTGGACGACATGATTGACACCGGTGGTACGGTAATAGCAGCAGCTAAACTACTCCGGGAACACCATGCCAAAAAGGTCTGTGTCATGGCCACCCATGGTTTGTTTAACGGTGAAGCACCGCAACGCTTCCAAAAGGCCTTTAACGAAGGCTTAGTGGACTATTTGTTTGTCTCCAACTCCATTCCTCAAACTAAGTTTAACCAGTGTCCCCAGTTTCAGGTAATTGATCTAGCACCGTTATTTGAAGAGGTGTTATTGTGTTACGCTAATAACTCTTCCATTTCTGCCATTTATACGCGCCACATTGAATGGATAAAAAAACACGTCTAA", "aa": "MDRHNHVVFSLSKTHDLVSRICQKLKMPMGLITHNEFADGETYIRFEESVRNKDVFIFQSTCAPVNDSLMELLIAIDALKRGSAKSITAILPYYGYARQDRKTMGREPITSKLVADLLTTAGVSRVALTDIHSDQTQGFFNIPVDTLQTYHVFLTRTVELLGKKDLVVVSPDYGGVKRARLIATSLELPLAIIDKRRPAHNVAESINVLGEVANKNCLIVDDMIDTGGTVIAAAKLLREHHAKKVCVMATHGLFNGEAPQRFQKAFNEGLVDYLFVSNSIPQTKFNQCPQFQVIDLAPLFEEVLLCYANNSSISAIYTRHIEWIKKHV", "aa_hexdigest": "e36318e5a5021916de51f95cb868152d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD649C", "ncbi_nrp_id": "WP_014574862.1", "uniref100_id": "UniRef100_A0AAV5NA32", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD649C", "RefSeq:WP_014574862.1", "UniRef:UniRef100_A0AAV5NA32" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NA32", "uniref90_id": "UniRef90_P75044" }, "psc": { "uniref90_id": "UniRef90_P75044", "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_P47304", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948", "go_ids": [ "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ] }, "pscc": { "uniref50_id": "UniRef50_P47304", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47304" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prs" ], "id": "CNOCLNGIAL_200", "locus": "GCA000143945_00160" }, { "type": "cds", "sequence": "contig_1", "start": 87873, "stop": 87977, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATCTGGTTGGCCAAACCTAAGAAAAAATTTGATAAACGTGAAGCCATCAAAAGTAAAACGATCCAGCGCGAATTGCGCCAACAATATGGATCGCCATAA", "aa": "MEIWLAKPKKKFDKREAIKSKTIQRELRQQYGSP", "aa_hexdigest": "d0f983ba3b409ef37fa5a900a17c06b4", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4132.832400000001, "isoelectric_point": 10.21090564727783 }, "id": "CNOCLNGIAL_201", "locus": "GCA000143945_00161" }, { "type": "cds", "sequence": "contig_1", "start": 87979, "stop": 88317, "strand": "-", "frame": 3, "gene": "smpB", "product": "SsrA-binding protein", "db_xrefs": [ "COG:COG0691", "COG:O", "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929", "KEGG:K03664", "SO:0001217", "UniRef:UniRef50_P75043", "UniRef:UniRef90_P75043" ], "nt": "ATGCGAGTACTTGTCAACAATCCCAGAGCGCAATATGACTATTACCTTTTAACGGGTTATTGTGCTGGCTTAGTCTTAAAAGGTAGTGAAGTCAAATCGCTAGCTTTAGGGCAAGGTAGCTTAAAGGAAGCCTATGTTTTTATTGACAAGCACGAGGTCTATATTAAAGATTTTAGCATTTCGCCCTATGCCTTTTCAGGCGAGTTCAACCACCCCTTCAAACGGGTGAAAAAGCTCCTTTTAAACCGGAATGAGATTAAACAAATTACGGCACGCCAAAAGCAAGAAGGACTTTCCATTATTCCACTTAAAGTGTTCTTTAAAAAATGGCAAAATTAA", "aa": "MRVLVNNPRAQYDYYLLTGYCAGLVLKGSEVKSLALGQGSLKEAYVFIDKHEVYIKDFSISPYAFSGEFNHPFKRVKKLLLNRNEIKQITARQKQEGLSIIPLKVFFKKWQN", "aa_hexdigest": "05827de51f275dc7cd7ef41193c5d399", "start_type": "ATG", "rbs_motif": null, "genes": [ "smpB" ], "truncated": "5-prime", "pseudogene": { "start": 87873, "stop": 88317, "observations": { "insertion": [ 87996 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75043", "query_cov": 0.9642857142857143, "subject_cov": 0.7346938775510204, "identity": 1.0, "score": 216.0, "evalue": 1.16e-70, "gene_start": 1, "gene_end": 108, "reference_start": 1, "reference_end": 108, "reference_sequence": "MRVLVNNPRAQYDYYLLTGYCAGLVLKGSEVKSLALGQGSLKEAYVFIDKHEVYIKDFSISPYAFSGEFNHPFKRVKKLLLNRNEIKQITARQKQEGLSIIPLKVFFKNGKIKMEIWLAKPKKKFDKREAIKSKTIQRELRQQYGSP" }, "paralog": false, "identity": 1.0, "score": 289.0, "evalue": 7.42e-102, "description": "Frameshift due to insertion around 87996.", "psc": { "uniref90_id": "UniRef90_P75043", "gene": "smpB", "product": "SsrA-binding protein", "uniref50_id": "UniRef50_P75043", "cog_id": "COG0691", "cog_category": "O", "kegg_orthology_id": "K03664", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75043", "UniRef:UniRef50_P75043", "COG:COG0691", "COG:O", "KEGG:K03664", "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929" ] }, "pscc": { "uniref50_id": "UniRef50_P75043", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75043" ], "product": "SsrA-binding protein" } }, "id": "CNOCLNGIAL_202", "locus": "GCA000143945_00162" }, { "type": "cds", "sequence": "contig_1", "start": 88324, "stop": 88701, "strand": "+", "frame": 1, "gene": "wcaA", "product": "putative glycosyltransferase MG060-like protein", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.-.-", "GO:0016757", "SO:0001217", "UniRef:UniRef50_P47306", "UniRef:UniRef90_P75042" ], "nt": "ATGAAAATCTCTGTTATTATCTCCACTTACAACTGTGGTGCTTTAATTGTGAAAGCACTATGTTCATTAGTGTCAAACCAAACACCAGCATGTGAGTTAGAAGTGCTTGTGATTGATGATGGATCCATTGATAATACTAGGCAAATTATCAAAAAATTTCAAGCAAAAGTTAGCTTTACTTTAAAGTACTTTTACAAAAAAAACGGAAATTGGGGTAGTGTAATTAACTATGTCAAGGAAAACCGTTTGGCCAACGGCGATTGGATTACTGTTTTAGACAGTGATGATACCTTAAAACCTAATACACTCAACAAGTTGGCTAATTTAGTGGAAAAAGCGGATTATGACTTAGTAGTTTTTGACTACACTAAGTGCTGA", "aa": "MKISVIISTYNCGALIVKALCSLVSNQTPACELEVLVIDDGSIDNTRQIIKKFQAKVSFTLKYFYKKNGNWGSVINYVKENRLANGDWITVLDSDDTLKPNTLNKLANLVEKADYDLVVFDYTKC", "aa_hexdigest": "f298052b3cb4ab81b483fdf9067e1fd2", "start_type": "ATG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 88324, "stop": 89004, "observations": { "mutation": [ 88699, 88741, 88972 ], "stop": [ 88699, 88741, 88972 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75042", "query_cov": 1.0, "subject_cov": 0.4180602006688963, "identity": 1.0, "score": 249.0, "evalue": 2.29e-81, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MKISVIISTYNCGALIVKALCSLVSNQTPACELEVLVIDDGSIDNTRQIIKKFQAKVSFTLKYFYKKNGNWGSVINYVKENRLANGDWITVLDSDDTLKPNTLNKLANLVEKADYDLVVFDYTKCWKKIKLKIHTYPTWWKNMTRELQKQTPFCIPLGKFLKRNLFYKLPKLKEKVSFQDALYTASSLKLAKKVRHVNQSGGNYHFKRAGNSMSIPWNIKRFSAELDICKDLIRLNAQEIALVHLLRQQFRVQLKEKQIQLAVTRDFNFSGFSWYTRCFLWMVYQTMLKRYFYLQTTKQ" }, "paralog": false, "identity": 0.9867256637168141, "score": 447.0, "evalue": 1.16e-161, "description": "Internal stop codon at 88699, 88741, 88972. Nonsense mutation around 88699, 88741, 88972.", "psc": { "uniref90_id": "UniRef90_P75042", "gene": "WcaA", "product": "Uncharacterized glycosyltransferase MG060 homolog", "ec_ids": [ "2.4.-.-" ], "uniref50_id": "UniRef50_P47306", "cog_id": "COG0463", "cog_category": "M", "go_ids": [ "GO:0016757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75042", "EC:2.4.-.-", "UniRef:UniRef50_P47306", "COG:COG0463", "COG:M", "GO:0016757" ] }, "pscc": { "uniref50_id": "UniRef50_P47306", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47306" ], "product": "Uncharacterized glycosyltransferase MG060" } }, "id": "CNOCLNGIAL_203", "locus": "GCA000143945_00163" }, { "type": "cds", "sequence": "contig_1", "start": 88750, "stop": 88974, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCCGGGAACTACAAAAGCAAACACCGTTTTGTATTCCTCTAGGTAAGTTTTTGAAACGCAACCTGTTTTACAAGTTGCCCAAGCTCAAAGAAAAGGTGAGTTTTCAGGATGCCTTGTATACGGCTAGTAGTTTAAAGCTCGCTAAAAAAGTGCGTCATGTTAATCAGTCTGGTGGCAACTACCACTTTAAGCGTGCTGGTAATTCAATGAGTATTCCCTGA", "aa": "MTRELQKQTPFCIPLGKFLKRNLFYKLPKLKEKVSFQDALYTASSLKLAKKVRHVNQSGGNYHFKRAGNSMSIP", "aa_hexdigest": "d2894568273e4512d45f3cb13c0b2d79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8528.9778, "isoelectric_point": 10.389483070373537 }, "id": "CNOCLNGIAL_204", "locus": "GCA000143945_00164" }, { "type": "cds", "sequence": "contig_1", "start": 88999, "stop": 89223, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACATTTGTAAAGACTTAATTCGTTTAAATGCTCAGGAAATTGCCCTAGTGCACTTATTGCGACAACAGTTTCGGGTGCAACTTAAAGAAAAGCAGATACAACTAGCGGTTACCCGTGACTTTAACTTTAGTGGGTTCTCTTGGTACACCCGCTGCTTTTTATGGATGGTATACCAAACGATGTTAAAGCGCTACTTTTACTTACAAACAACTAAACAATAA", "aa": "MDICKDLIRLNAQEIALVHLLRQQFRVQLKEKQIQLAVTRDFNFSGFSWYTRCFLWMVYQTMLKRYFYLQTTKQ", "aa_hexdigest": "43eac58589072e5fdffa7564562ad337", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9156.707199999993, "isoelectric_point": 9.755694389343262 }, "id": "CNOCLNGIAL_205", "locus": "GCA000143945_00165" }, { "type": "cds", "sequence": "contig_1", "start": 89329, "stop": 89787, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTCATGAATGCCTTAATGGTGATTGTAATCGTATTGTTTGCAGCCACTCTAGGTGTGGGTACTGCTGTTGGCTTTGCCTTTGTGGCGATTGCAACCTTTATTGGTGGTTCGTTTGCTTGGAGTATGCAAAGTACCATTTTGATCCTCCCGCACGAATTCAAAGAGTACAAGCGTAGTGAAGTGTCCGTGTTGTTTGGTTACATCTGGGGCTTTGGTTATGTGATCTACACCGCCTTTGACATTACTAACTCAATGTTCTTAGAAGCACCGAAACTAGCAAACCCTGGAATGAAAGGAGTAAGCATCCTCCCAGGGGCAATTGCTGGTGTGGCGCTGTTTGCTGGTTTGTTGTTAGCAGCCATTGCGATTGTGGTAACACTGCCATCTTCGTATCTTAAGAATGGCGATGAACTGGTAAGTGAAATGACCAAAAAGTGGAAGTTAAACCAGTGA", "aa": "MFFMNALMVIVIVLFAATLGVGTAVGFAFVAIATFIGGSFAWSMQSTILILPHEFKEYKRSEVSVLFGYIWGFGYVIYTAFDITNSMFLEAPKLANPGMKGVSILPGAIAGVALFAGLLLAAIAIVVTLPSSYLKNGDELVSEMTKKWKLNQ", "aa_hexdigest": "4da9470b75af747b10a78319ffb11673", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16400.338599999985, "isoelectric_point": 7.947485160827637 }, "id": "CNOCLNGIAL_206", "locus": "GCA000143945_00166" }, { "type": "cds", "sequence": "contig_1", "start": 89797, "stop": 89976, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTCCAGCGAGTTCGTTTAATGTTAAAGATGGTGTTAAAACATTAAAACCACTAGAGGGTGCTGGTGGTTACTTTGCCGGTCTCCCAACCTTAGCAATCCTCTGGGTATTAGGTTATGGTATGGGTTACATGGTGTTTTCACCATTTAACAAAACGGTTTACGATCGCAAGCGTTGA", "aa": "MSPASSFNVKDGVKTLKPLEGAGGYFAGLPTLAILWVLGYGMGYMVFSPFNKTVYDRKR", "aa_hexdigest": "309e4e3c168cbb206e83d532e66bab84", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6448.490000000002, "isoelectric_point": 9.69728603363037 }, "id": "CNOCLNGIAL_207", "locus": "GCA000143945_00167" }, { "type": "cds", "sequence": "contig_1", "start": 90058, "stop": 90735, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTCCTGAAGTTTGGTTACCGTTATGCGGTCATGATTATGATGGGGCTAATGTGTCTCTGTTTCCCCTTCTTAATTATTGGTGATCCATTAGGGGGAAATAACCAGTTAACTTTATTGCGACCACTTTCAAAGGAAGTAATGGGTAAACTTTCTAGTCTATCTTCGCAATTACATGAAGGTCAATTGTTGGGGCCTGTTATGGCAGAAGGCAAGACCATGTTAGCAGATGGTCAAACTATTGATTTAGTTCAAGGTTTAGATAAAAATTTAATTGGTACCAGTTCATCAATTGCTGGTTATGCGTTGTTTATTATTTTCCGTTCCACGATTGCTATTGGTGGTACAACTTTAGTAACTTACTCCCAACCGTTAATTGCTTCACTTTCAACGCAACGTCGTAAGTCAGTGTTATCCAACGCGAACCTCTGGGGCTTTAACAGTGGGATTGTAGTTGCTTTTGTACCGTTCTTATTCCAATCTGTTCAACAAGCTGGTACTAAGTACTGGGTCTTTATCCTAACTGCTTTAATCTTGATTGGTTTTGGCATTCTCTGTGTGTTTGCTTGGTTTGAAAAGCAAATGGATCCGTTTATGCCCCAAAAGCAAACCAAGGAACAAATGCAGTTGGGTAACCAACCAAGTGCTGGTGATATCTTAAAGCGAAAAGCGACCTGA", "aa": "MVLKFGYRYAVMIMMGLMCLCFPFLIIGDPLGGNNQLTLLRPLSKEVMGKLSSLSSQLHEGQLLGPVMAEGKTMLADGQTIDLVQGLDKNLIGTSSSIAGYALFIIFRSTIAIGGTTLVTYSQPLIASLSTQRRKSVLSNANLWGFNSGIVVAFVPFLFQSVQQAGTKYWVFILTALILIGFGILCVFAWFEKQMDPFMPQKQTKEQMQLGNQPSAGDILKRKAT", "aa_hexdigest": "99fdaee3a16aeb0639d4665cbff6b736", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24641.918399999988, "isoelectric_point": 9.454304695129395 }, "id": "CNOCLNGIAL_208", "locus": "GCA000143945_00168" }, { "type": "cds", "sequence": "contig_1", "start": 91559, "stop": 91993, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCCCAAAAGCAATCAAAAACCAATCAAAGCTTGAGTGTGAGACCAAGTGCGTTATCAATTTTGAAACGCAAAACAACTTGGAAGCTGTTGGCAATAGCTGGTGTAGGTACAATTCTCTTAATTAACCCGTTAACCCAAACCTGGTTTAACAGCTTGTTAGCTATTTCAGGTGCTAAAAAAGCAATTATTCCAACTGCCCGTCCAATCTTATTAATTCTCTGGGTAATGGGTTACCTCTTGGGTTACTTCTTACTGTCACCGTTTAACAAAACAATTTATGACAAGAAGCGTTGGCTCCACTTTATCTTTACGGCCAACGCCGTGTTGGTGTTGTTAATAGTAATCTTTGCTGCAACGCTTGGTTTAAATACTGTGGTTGGCTTTACGTTTGTGGGTATCTTTACCTTTATTGCTGGTGGTTTTGGTTGA", "aa": "MLPQKQSKTNQSLSVRPSALSILKRKTTWKLLAIAGVGTILLINPLTQTWFNSLLAISGAKKAIIPTARPILLILWVMGYLLGYFLLSPFNKTIYDKKRWLHFIFTANAVLVLLIVIFAATLGLNTVVGFTFVGIFTFIAGGFG", "aa_hexdigest": "c9bc3d292f9563757b86074e006a3536", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15868.987999999968, "isoelectric_point": 10.94539394378662 }, "id": "CNOCLNGIAL_209", "locus": "GCA000143945_00169" }, { "type": "cds", "sequence": "contig_1", "start": 92055, "stop": 92225, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACGTTACAACTCAAACCCAGTTAGCAGTCGCAAAGCGGCCAACACCTGGAATTAAGAAAGGTGCTGCCACAATTACAAGTCCTACGTTAAAACCTCAGAAGTTAGCATTCGATAAAATCGATTTCTTGCGGTTTGAAGAAAGGGAAGCAATAATTGGTTGCGCATAA", "aa": "MNVTTQTQLAVAKRPTPGIKKGAATITSPTLKPQKLAFDKIDFLRFEEREAIIGCA", "aa_hexdigest": "7146cd238a3a90282f692ff450166441", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6116.118499999999, "isoelectric_point": 9.743251991271972 }, "id": "CNOCLNGIAL_210", "locus": "GCA000143945_00170" }, { "type": "cds", "sequence": "contig_1", "start": 92153, "stop": 92584, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGTGAAGTTTGGTTACCGTCATGCAGTGATGATCATGATGGGACTCATGTGTGTTTGTTTTCCTTTCTTAATTATTGGTAGTCCGTTAGGTGGTCATAACGAGTTGACCTTGTTGCGACCGGCTTCAAGTGAAGTAATAAGTAAACTTACTAAAATTTCTTCCAGTTTACAACAAGGTCAACTGTTAGGTCCTGTTCAAGTGGGTAGTCAAACTATGTTGGCTGACGGCACTCCTGTTAGCTTAATTAAAGGAATTAACGGCAACGAAATTGGTACCAGTGCGTCAATGACTGGTTATGCGTTTTTCATTATTTTCCGTTCTACGATTGCTATTGGTGGTACGACCTTAATTGCTTATGCGCAACCAATTATTGCTTCCCTTTCTTCAAACCGCAAGAAATCGATTTTATCGAATGCTAACTTCTGA", "aa": "MVVKFGYRHAVMIMMGLMCVCFPFLIIGSPLGGHNELTLLRPASSEVISKLTKISSSLQQGQLLGPVQVGSQTMLADGTPVSLIKGINGNEIGTSASMTGYAFFIIFRSTIAIGGTTLIAYAQPIIASLSSNRKKSILSNANF", "aa_hexdigest": "c22c84bb559ba32bb83c1a8ea59c2d6a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15126.707199999984, "isoelectric_point": 9.757821846008298 }, "id": "CNOCLNGIAL_211", "locus": "GCA000143945_00171" }, { "type": "cds", "sequence": "contig_1", "start": 92630, "stop": 92788, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTTTGTGATTGAATGGTTTGTGATTGACTTCATTAGAGGTAGTGCGGCTTCCCTTTTAACTGAACAAACTAGTGCTGTTCCCCAGTACGGTGGTTGGTTCAGTTCTTTTTTTGTAGCGGACGCTGGTTTTATTCCAGGTCAAGCTACTAACTGA", "aa": "MLFVIEWFVIDFIRGSAASLLTEQTSAVPQYGGWFSSFFVADAGFIPGQATN", "aa_hexdigest": "f91f5412a1721c9bc8b97dfb63678239", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5691.3793, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_212", "locus": "GCA000143945_00172" }, { "type": "tRNA", "sequence": "contig_1", "start": 93028, "stop": 93116, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 76.9, "nt": "GGGAGCGTACTCAAGTGGTTAAGAGGACACCCTGCTAAGGTGTTAGATCGTTCTACGGTGCGTGGGTTCGAATCCCACCGCTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 93081, 93083 ], "id": "CNOCLNGIAL_34", "locus": "GCA000143945_00173" }, { "type": "cds", "sequence": "contig_1", "start": 93347, "stop": 94414, "strand": "+", "frame": 2, "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "db_xrefs": [ "COG:COG1762", "COG:GT", "GO:0005351", "GO:0005886", "GO:0009401", "GO:0016301", "GO:0022877", "GO:0090563", "SO:0001217", "UniRef:UniRef50_P47308", "UniRef:UniRef90_P75039" ], "nt": "ATGTCAAAACTGGCATTGCCCCACATGCAAAGTGCTACCGTGTTAAAACCGTTTGTGGCGGTGTTTAAGGTCAACAATGTGGACTGGCAATCCTTAGACAACCAACCGGTTAAACTAATCTTTTTAATTGGCGTACCCAAAGATCAAGGCAACCTCCACTTGGAATTTATTTCGCAGTTTTCCAAGTTAATGTTGCAGGACGAGTTTGCCAACAAAGTGCCCAACATTCGCAGCTTTAACGGGTTGATCAATTTAATTGACAGTTTTCAACAAACTGCAGTTGCAAGTCAACCTGTTGTTAACGAAGCAGCAGCTCAGACTGAAGAGCCAAAGGACACAAACACTCAGTATGACTTTGTGGCGGTAACGGCGTGTCCCACCGGGATTGCTCACACCTTTATGGCCAAGGAAGCGCTGGAAAAGTTTGCCCGCGATCACAACCTCAAAGTTAAGGTCGAAACTCAAGGAACTGATGGGATTCAGAACCAGTTAACCGAGTCTGATTTAAACAATACCAAAGGCATTATTTTGGCCTGTGACCGCTTAATTGATTTAACGCGTTTTTACGGTCACGCAAATGTGGTGGAAGTGTCCACAACCAAAGCAATTAAAACACCACAAACAGTTTATGACCAAGTGGTAAAGAAGGAAGGCAAACTACTCGGTAATAAGTCCAGTGATAGTGCTAGTCAAACGGAACTCAAAGAAACCACTGAACAGCTCTCGTTTAAAGACTTTCACAAACGGATTTACCGCGCCATTTTAAGTGGGGTTTCTTACATGCTGCCATTTGTCGTGTTTGGTGGCATCTTAATTGCAATTGCCTTTTTAATTGACATTAATAATGCTGGCAATGCTGGTAAACAATTTGGCTCTAAAGATCCCATAGCTAACTGGTTTAAAACACTCGGTGGGTTGTCGTTTGGTTTAATTGTTCCCATTTTAAGTGCTTACATTGCCTTTGCTCTAGTCGGCCGGCAAGGGTTACTGCCCGGCTTTATTGTGGGGTTAATTTCAGCTGGTAAGTTTTTACTCAACATTGACATTGTTACTGGAAAAATTGATTGA", "aa": "MSKLALPHMQSATVLKPFVAVFKVNNVDWQSLDNQPVKLIFLIGVPKDQGNLHLEFISQFSKLMLQDEFANKVPNIRSFNGLINLIDSFQQTAVASQPVVNEAAAQTEEPKDTNTQYDFVAVTACPTGIAHTFMAKEALEKFARDHNLKVKVETQGTDGIQNQLTESDLNNTKGIILACDRLIDLTRFYGHANVVEVSTTKAIKTPQTVYDQVVKKEGKLLGNKSSDSASQTELKETTEQLSFKDFHKRIYRAILSGVSYMLPFVVFGGILIAIAFLIDINNAGNAGKQFGSKDPIANWFKTLGGLSFGLIVPILSAYIAFALVGRQGLLPGFIVGLISAGKFLLNIDIVTGKID", "aa_hexdigest": "0363d2a8b599c9665c25ea3afe23be4c", "start_type": "ATG", "rbs_motif": null, "genes": [ "fruA" ], "truncated": "both", "pseudogene": { "start": 93167, "stop": 94717, "observations": { "mutation": [ 93206, 94412, 94595 ], "stop": [ 93206, 94412, 94595 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75039", "query_cov": 1.0, "subject_cov": 0.5115273775216138, "identity": 1.0, "score": 688.0, "evalue": 1.56e-244, "gene_start": 1, "gene_end": 355, "reference_start": 61, "reference_end": 415, "reference_sequence": "MFKPLLSAELFFNWTAKDFKDKTSFLKQACRVLQDKNCIKEEQIALTALKEREAQITTGIMSKLALPHMQSATVLKPFVAVFKVNNVDWQSLDNQPVKLIFLIGVPKDQGNLHLEFISQFSKLMLQDEFANKVPNIRSFNGLINLIDSFQQTAVASQPVVNEAAAQTEEPKDTNTQYDFVAVTACPTGIAHTFMAKEALEKFARDHNLKVKVETQGTDGIQNQLTESDLNNTKGIILACDRLIDLTRFYGHANVVEVSTTKAIKTPQTVYDQVVKKEGKLLGNKSSDSASQTELKETTEQLSFKDFHKRIYRAILSGVSYMLPFVVFGGILIAIAFLIDINNAGNAGKQFGSKDPIANWFKTLGGLSFGLIVPILSAYIAFALVGRQGLLPGFIVGLISAGKFLLNIDIVTGKIDWATESKVSSGFFGAIFGGLLAAVLIIVQQRYIYRKLPQALQGIKNILFIPLLGTLVTAALFWVINIPLIYLNYGLSKFLQIMDKPYLAPLLGLVIGLMMCFDLGGPVNKAAYVFGVVSLESQNSGTVAMASAILSGMVPPLGIAIAATIRKQCFDKEELPAAYACYVMGLSFISEGAIPFVAKRPKIMLAANLIGGAVCGVLTGAFALTIRAPHGGVFVFALLKTNLEGIAGNTLQIGAGVGLALLALIVSSFISAGIIIGHNLLVVRKKTKQLVNTNA" }, "paralog": false, "identity": 0.9922480620155039, "score": 991.0, "evalue": 0.0, "description": "Internal stop codon at 93206, 94412, 94595. Nonsense mutation around 93206, 94412, 94595.", "psc": { "uniref90_id": "UniRef90_P75039", "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "uniref50_id": "UniRef50_P47308", "cog_id": "COG1762", "cog_category": "GT", "go_ids": [ "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75039", "UniRef:UniRef50_P47308", "COG:COG1762", "COG:GT", "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47308", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47308" ], "product": "PTS system fructose-specific EIIABC component" } }, "id": "CNOCLNGIAL_213", "locus": "GCA000143945_00174" }, { "type": "cds", "sequence": "contig_1", "start": 94478, "stop": 94597, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAATTATTGTGCAGCAACGTTACATCTATAGAAAATTACCGCAAGCATTACAAGGCATTAAGAACATTCTCTTTATTCCGTTACTAGGAACGCTAGTCACAGCAGCCTTGTTCTGA", "aa": "MLIIVQQRYIYRKLPQALQGIKNILFIPLLGTLVTAALF", "aa_hexdigest": "4a6316b7bf7b970071d88b097025d84a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4471.4836000000005, "isoelectric_point": 10.446408653259276 }, "id": "CNOCLNGIAL_214", "locus": "GCA000143945_00175" }, { "type": "cds", "sequence": "contig_1", "start": 94622, "stop": 95251, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTATGGTCTGTCCAAGTTCTTGCAAATTATGGACAAACCATATTTGGCTCCCTTACTTGGTTTAGTGATTGGACTCATGATGTGCTTTGACTTGGGTGGACCGGTCAACAAAGCGGCCTATGTGTTTGGGGTAGTTTCGCTAGAGAGCCAAAACAGTGGTACGGTTGCAATGGCTAGTGCTATCTTATCGGGGATGGTGCCACCATTGGGTATTGCCATTGCTGCGACCATTCGCAAGCAGTGCTTTGACAAGGAAGAATTACCAGCAGCGTATGCTTGTTATGTGATGGGATTAAGTTTTATTAGTGAAGGGGCGATTCCTTTTGTTGCTAAACGGCCTAAGATTATGTTAGCTGCTAACCTAATTGGTGGTGCTGTGTGTGGTGTTTTAACGGGAGCATTTGCGCTAACGATTCGTGCCCCTCATGGTGGCGTGTTTGTCTTTGCCTTATTGAAAACTAACTTAGAGGGCATTGCCGGCAACACACTCCAAATCGGCGCTGGGGTTGGCTTAGCACTTCTAGCGCTAATAGTATCCAGCTTCATTAGTGCTGGCATTATTATTGGTCATAACCTGCTTGTAGTGCGTAAAAAAACAAAACAACTAGTAAATACCAATGCTTAA", "aa": "MNYGLSKFLQIMDKPYLAPLLGLVIGLMMCFDLGGPVNKAAYVFGVVSLESQNSGTVAMASAILSGMVPPLGIAIAATIRKQCFDKEELPAAYACYVMGLSFISEGAIPFVAKRPKIMLAANLIGGAVCGVLTGAFALTIRAPHGGVFVFALLKTNLEGIAGNTLQIGAGVGLALLALIVSSFISAGIIIGHNLLVVRKKTKQLVNTNA", "aa_hexdigest": "d8d4f8ca229ac002fca436f86be1a120", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21653.737600000008, "isoelectric_point": 9.38996524810791 }, "id": "CNOCLNGIAL_215", "locus": "GCA000143945_00176" }, { "type": "cds", "sequence": "contig_1", "start": 95331, "stop": 96146, "strand": "+", "frame": 3, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "db_xrefs": [ "COG:COG1105", "COG:G", "EC:2.7.1.56", "GO:0005524", "GO:0005829", "GO:0008662", "SO:0001217", "UniRef:UniRef50_Q49396", "UniRef:UniRef90_P75038" ], "nt": "GTGTTAACACCCGGTGGTAAGGGAATTAACATGGCGATTGTAATGGCTTTATTTGGCATCAAACCAACCGTGTTAACCTTTTTGGGTCAACCTACTAAAGATTTATTCTTGCAGTTGTTAAAACCGTACCAACTGGACTTAGTTAGCTTTCCAGCGACTACCCAAACACGGATTAACGTGAAGCTGTTGGATGGTGCCCAAACAACTGAAATTAACGATGTCACCCCGTTAATGGAGGAACAAGCAGTCCATGAAATGATTGCTTACCTTAAGGCTAACGTTAAGCCCAATGACCTGTTGGTGTTGAACGGCCGTTTTTTACAACGCGATTTAGTTAAGTTGTTAGATGTTGCTTTTAGCTTAACCAAGTATGTTGTATTAGATGTTGATGAACCTCAACTATTACAACTGTTAAACCAGCGCCAACCCTGGTTAATGAAACCGAACCGTGATGAGTTTGTAGCAATGGTTAATGCAAATAACAGTAACGTTGACCAACAAGAGTTGGTGCAGTTAATAAAGCAGTTTCAGACTACACAGAACTTATTAATGTCAGACGGCGCGCAGGGTGCTTACTTCTTTGATCAACAGCAACTGTTATTTATGGAAGCGATTCCTCCCCAACAGTTAGTTTCCACTACGGGAGCGGGAGATACATTGCTGGGTGTTTTCCTAGCTAACTTACTGTTAGATAAGGATCCAGTGGGTAGTTTAAAGGTAGCGGTTAACTATGCTAGTGCTACGATTAGCAAGCTCGGTGTGGTCAACAGTAATGACCAAATAGTGCTAAAAGCTACTAATTATTACTATTTATAA", "aa": "MLTPGGKGINMAIVMALFGIKPTVLTFLGQPTKDLFLQLLKPYQLDLVSFPATTQTRINVKLLDGAQTTEINDVTPLMEEQAVHEMIAYLKANVKPNDLLVLNGRFLQRDLVKLLDVAFSLTKYVVLDVDEPQLLQLLNQRQPWLMKPNRDEFVAMVNANNSNVDQQELVQLIKQFQTTQNLLMSDGAQGAYFFDQQQLLFMEAIPPQQLVSTTGAGDTLLGVFLANLLLDKDPVGSLKVAVNYASATISKLGVVNSNDQIVLKATNYYYL", "aa_hexdigest": "a6d4e6e3c937ecfd1a7eed9676f574e1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75038", "query_cov": 1.0, "subject_cov": 0.9033333333333333, "identity": 0.9890000000000001, "score": 516.0, "evalue": 4.38e-184, "valid": true, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "ec_ids": [ "2.7.1.56" ], "uniref50_id": "UniRef50_Q49396", "cog_id": "COG1105", "cog_category": "G", "go_ids": [ "GO:0005829", "GO:0008662", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_Q49396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49396" ], "product": "Putative 1-phosphofructokinase" }, "genes": [ "fruK" ], "id": "CNOCLNGIAL_216", "locus": "GCA000143945_00177" }, { "type": "cds", "sequence": "contig_1", "start": 96154, "stop": 96315, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574865.1", "SO:0001217", "UniParc:UPI0001DD64A0", "UniRef:UniRef100_A0AB38W8P1", "UniRef:UniRef50_A0AB38W8P1", "UniRef:UniRef90_A0AB38W8P1" ], "nt": "ATGTCAACTAAAGAACTAGCCAAACAGCCAAAGGTCAATCAAGAGAGGGAATCGATTTTGTTACCCGTTTTGCTAGCTGTTTTCAGTATGCTCTTCTTTATGCTACTGTTAGCTTTAGTGTTGTTGTACGCTATTCCAGGTAAAGTTAATAACTTGAACTAG", "aa": "MSTKELAKQPKVNQERESILLPVLLAVFSMLFFMLLLALVLLYAIPGKVNNLN", "aa_hexdigest": "d439b785fba30d91bfa41ab139de3492", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64A0", "ncbi_nrp_id": "WP_014574865.1", "uniref100_id": "UniRef100_A0AB38W8P1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64A0", "RefSeq:WP_014574865.1", "UniRef:UniRef100_A0AB38W8P1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8P1", "uniref90_id": "UniRef90_A0AB38W8P1" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8P1", "uniref50_id": "UniRef50_A0AB38W8P1" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8P1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5973.246899999998, "isoelectric_point": 9.398732948303223 }, "id": "CNOCLNGIAL_217", "locus": "GCA000143945_00178" }, { "type": "cds", "sequence": "contig_1", "start": 96383, "stop": 97489, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGCAACTTAATTAAAAACGTTTTTCGCTCTTTTCGCACCGCTAAGATTGCCTTAATTGCGCTTACCTTTTTAATCTTTGTCGCGGTTGGTAGTTTCGTGTTATTAAACAATACCGTTAATAACTTTAACGCTTCCTTTCAACACGTTACCGAACAAGGACAATTGTCTAACGCCATTATTAACGAGCGCTATGACTTTGGTAAACTCGAGTTCCAAAAAACGAGTAATGATGCTACTAGTTCTGCCCAACCTAGTGTTAGTACTCGAAGTACTTTAACTGCCCAAAAAGCAAATGGCGCTAATTCCAGTTTTACTCTGACCTTAACACCTGACTCACGGACTTCATTTATTAACAACGCACTGCAACTAAAACCGGATGTTTATAACAGTTTAGTTACCGTTACTTTTAGTTATGGCAGTGAATCTGATCAAACCAAGAAAACCCAAATAGAAGAACAGTCCAAGTTAATTGCCGCAAACAATTTAAGCCGGGCATTACAAACCGATAAGGAGTTATTAGTTACGGGACAGTTAGAACAGTTAAAGGCTACCTTTCGCACTTATAAGGCAATTAACATTACTGATCAAGGAGTGTTCAAGAAGCTCATAGCCTCTGAACCGACCGATCAAGTTAACCGTCTCGTTCTGTTTGACGGACATCAACTAGCACGTTCCCGCCAAGTCGAATTTAACGAGCTGGTTAGTCAGTTTACCCAGGTGCAAGCTAAGGGTAAAGATCAGTTATCCACTACTCTAAAGAATGGTAATTACGAACAACTTTTGCAAGCGATCGCTAACAACTTTGGTAGTAGTGAAACTGAGTTACTTAAAGAAATTCAAGATGCTTTAAAGCAAACGAATAGTTCCACCACCCTTGCCCAAACCCAAAGTAGTCAACAATTTAGTAAGTTAAAAAACCTGTTTGATTCTGGTAGTACCTTTACTAACATTGCCGGCAAACTCACGTTGCAGTGGATGGAAGGTACAAACAAAAAGCAACTGGTAATCTTTGACCCGAGTAGCTATGAAACGGTAGTAGCACCGGGTAACTGGAGTTACGAACAGCAACAGGGCAAAGCGGTGTATCAGGACATTAACCACTGA", "aa": "MRNLIKNVFRSFRTAKIALIALTFLIFVAVGSFVLLNNTVNNFNASFQHVTEQGQLSNAIINERYDFGKLEFQKTSNDATSSAQPSVSTRSTLTAQKANGANSSFTLTLTPDSRTSFINNALQLKPDVYNSLVTVTFSYGSESDQTKKTQIEEQSKLIAANNLSRALQTDKELLVTGQLEQLKATFRTYKAINITDQGVFKKLIASEPTDQVNRLVLFDGHQLARSRQVEFNELVSQFTQVQAKGKDQLSTTLKNGNYEQLLQAIANNFGSSETELLKEIQDALKQTNSSTTLAQTQSSQQFSKLKNLFDSGSTFTNIAGKLTLQWMEGTNKKQLVIFDPSSYETVVAPGNWSYEQQQGKAVYQDINH", "aa_hexdigest": "c29dac8e35c6acd2a0f398e27f2abc18", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 40980.39510000004, "isoelectric_point": 9.000382804870608 }, "id": "CNOCLNGIAL_218", "locus": "GCA000143945_00179" }, { "type": "cds", "sequence": "contig_1", "start": 97559, "stop": 97816, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGTCGACAAAATTGAGTACCTCATCTTGGGAGTGGGAATTACACCAGACTTTGTCTACCCCGTCTTTAGTGAATCACTGTTAATTCCCAATACGGCTAACGAGCAGTTGTATTATGTCAACCAAACTGGTTATGAACGTACCGCTAGCTCCTTTTTAACTAATCCCACCGAAACTGGCATAGTAGCACGGTTACCTAACCTCAACCATGACCTTGATGTAATTAACCAGTGGGCTGTTAAAAACATGTCCTGA", "aa": "MLVDKIEYLILGVGITPDFVYPVFSESLLIPNTANEQLYYVNQTGYERTASSFLTNPTETGIVARLPNLNHDLDVINQWAVKNMS", "aa_hexdigest": "2f3edb560e1258c6123ebab8a737b2cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9577.764099999995, "isoelectric_point": 4.395041084289551 }, "id": "CNOCLNGIAL_219", "locus": "GCA000143945_00180" }, { "type": "cds", "sequence": "contig_1", "start": 97958, "stop": 98275, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "GTGGCCATCTTTGTCACCATTTTAATCTTGGTTAGTTACCTCAAAAAGAACACGGAACAAATCGGCATCTTAAAGGCTAATGGGGTGAGTGGCCGGAAGATTAACTTAAGCCTGTTAATCTTTAGTTTAATCCCGGGGATAGTGGGGTCCTTTATTGGTTATACGTTAGGTATTAGTATGCAAAACGTGGCCATTAACCTCTTTAGTAACTACTGGTTTATCCCCACGCAAACTTCGAGTTTCTCGGTGTTAGGGTTACTCTTCTTTGCCTTATTAGTGGTTCTTATGATGAGTGGGATTAGTCTGTTTGTGGGGTGA", "aa": "MAIFVTILILVSYLKKNTEQIGILKANGVSGRKINLSLLIFSLIPGIVGSFIGYTLGISMQNVAINLFSNYWFIPTQTSSFSVLGLLFFALLVVLMMSGISLFVG", "aa_hexdigest": "5f22642b17d0925359a7c7a29844e4e6", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 102, "aa_cov": 0.9619047619047619, "hmm_cov": 1.0, "evalue": 6.221903269209277e-16, "score": 54.036476135253906, "start": 2, "stop": 102 } ], "seq_stats": { "molecular_weight": 11409.65179999999, "isoelectric_point": 9.873542594909669 }, "id": "CNOCLNGIAL_220", "locus": "GCA000143945_00181" }, { "type": "cds", "sequence": "contig_1", "start": 98381, "stop": 98596, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCAGATTTCGTGTCGCTTTAGCCTTTAATGCGCCGTGGAAACTGGTCTTTTTAACCTTAATGAGTGCACTAACGATGATGATCCTAAACCTTAGTTTTGCCACTAAGGATGCCTTTCAGGTAGCACAAACAAAGACTACTTTAACCCAATCGAACCACCAGTACGAGTTTGAACTAGCATCGCCAACGGAGCAGAGTGGCTTGCTCAAGTGA", "aa": "MTRFRVALAFNAPWKLVFLTLMSALTMMILNLSFATKDAFQVAQTKTTLTQSNHQYEFELASPTEQSGLLK", "aa_hexdigest": "721e075f6949e99f2db9ca17c102f9d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8043.3189999999995, "isoelectric_point": 9.397636985778806 }, "id": "CNOCLNGIAL_221", "locus": "GCA000143945_00182" }, { "type": "cds", "sequence": "contig_1", "start": 98795, "stop": 99397, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACTATTCACTGGGTTTTGGTAACGTTTCTTCCAATCCGTGGAAGCTGAGTGAAACATTAATGCCCGCCAATCAAGCATCCGCTTCCAACACGGCTTTCCAAAACTTTTTAAAGGCGATTATTGTAATTGACCCTAACGAGGGCGGTAAGTACATTAAACAAACTAAGGATGAAGTTACTGTGCGTTTTTTATACGAAATAGACAGTAGTAAAGCTTTAAAAAACGGCAACACCAAATCAGTTAATAAAATACAAAAAAGTACCGCAGCACCCCAAGCCCAACAAGGACAAAGTAACCAGTTAATGCTTGACAATGATTTTGCTAAATTCTTGTACAAGCAGTTTAACCTAATCAAGGAAGGGGGCAAAGCAGATCCGGTTGATCTCAATAACTTTGACTTTGCTAATCAGAATCAGATTAAGCGTTTTTACAGTAAATACAACGCCTTACCACCGGTAGATTACAAGCTGGCCTTTAACGTGATCGGTCTCCCTAAAGAAACAATTGCCAACCAAAAGGATGCGCCCCTGTTTGGCTTTTTAACCTTGCAAGCGCAACTGGGCAATGACGCGATTAAGATTAAGGGGATTAAAGACTGA", "aa": "MDYSLGFGNVSSNPWKLSETLMPANQASASNTAFQNFLKAIIVIDPNEGGKYIKQTKDEVTVRFLYEIDSSKALKNGNTKSVNKIQKSTAAPQAQQGQSNQLMLDNDFAKFLYKQFNLIKEGGKADPVDLNNFDFANQNQIKRFYSKYNALPPVDYKLAFNVIGLPKETIANQKDAPLFGFLTLQAQLGNDAIKIKGIKD", "aa_hexdigest": "2813de51a8f8e0d84be81da9514a6e04", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22253.004199999996, "isoelectric_point": 9.204554176330568 }, "id": "CNOCLNGIAL_222", "locus": "GCA000143945_00183" }, { "type": "cds", "sequence": "contig_1", "start": 99425, "stop": 100543, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F", "UniRef:UniRef90_UPI0011815B2F" ], "nt": "GTGTTGCGTACCAATGAGGACGAGATTATTAACCAGCGCTTGTTTAGTAAACCCAACGGTATTATTATCAACAGTTCAGCTGCTAAAAAGTACCGCTTAAAGGAAGGTAGTCAGCTCCATGTCAAAATTCACAACGGTTACAAACGAGTGAATGCTAAGTTAGTTAACCAAGATCCCACTTTAACTGCCACCTTTAACATTGTTGGCATTAACAATTCGGTGCACGAACCGGAGTTTTACACGAGTTACAAAACGGCAGCAACACTATTGCAATACCCCAATGAATGGATCGAAAAGCAGTTACCCTTTAACAGCTTTTATGCCAATTCGATCTTGCCCTTTACCCAGTCTACTTCCCTCTTTTCCGAATCTGGCTTATTCCCTGGTACCAGTAGCTTTGCCGCTAACAACACGGTGCTTACTGAAGTAATTAAGAAAACGATTGAAGGTGGTAAAACTAACAGTAAAACTGATGTGCAAAAGCGCGCTGCTCAAGCTACTGCTAACGGTAAGGATAACAGTAGTCAAAACAACAAGATGAATTATGAGCGCTTACAAAAGGCACTGGGCATTACCAGTGATTTAGATAGCTCTAAAGCCGGTGAATACGCCACCATCTTAGAGCGGGTGTATGATGGTTTGCCATACAACTCCACTATTAGCTTTATTAGTAATGTGCAGGCCAACGATGCCTTGTTTGCTAACATTGCTAACACCACCCAGCAAATCCAAACAGCGGCCATTGGCATTATTATTCCGATTATTATGCTAATTGTGTTGTTAGTATCAACTACCCTAATTCAGGAATTGAAGAAGATTGCGATTCGTTTAAAAGCATTGGGCTATTCCAACCCGAAGATCTTGCTGTCCTTCTTGTCGATTTACCTCCCGTTATTTATCTTTGGTTTGTTAATTTCGACCCCAATTTCGATTTATCTAATTGCACTCCACAATCAAGTTATTTTCTCCAGTGCTGCCATCTTACTGGAAGCCACATTAAACTTCCCCACGGTCATTCTGTCGATGTTAGTGTTAAGTGGGGTGTTATCAATTACCTTTGTGTTGAATTGGTTGGAGTTGAACAAAATCAAAATTGCCAAGGAGATTAAGAATAGCTAA", "aa": "MLRTNEDEIINQRLFSKPNGIIINSSAAKKYRLKEGSQLHVKIHNGYKRVNAKLVNQDPTLTATFNIVGINNSVHEPEFYTSYKTAATLLQYPNEWIEKQLPFNSFYANSILPFTQSTSLFSESGLFPGTSSFAANNTVLTEVIKKTIEGGKTNSKTDVQKRAAQATANGKDNSSQNNKMNYERLQKALGITSDLDSSKAGEYATILERVYDGLPYNSTISFISNVQANDALFANIANTTQQIQTAAIGIIIPIIMLIVLLVSTTLIQELKKIAIRLKALGYSNPKILLSFLSIYLPLFIFGLLISTPISIYLIALHNQVIFSSAAILLEATLNFPTVILSMLVLSGVLSITFVLNWLELNKIKIAKEIKNS", "aa_hexdigest": "e2a1e0215a876d97c17815cb3d841804", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0011815B2F", "query_cov": 1.0, "subject_cov": 0.8551724137931035, "identity": 0.997, "score": 679.0, "evalue": 6.56e-245, "valid": true, "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI0011815B2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0011815B2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F" ], "product": "ABC transporter permease" }, "genes": [], "id": "CNOCLNGIAL_223", "locus": "GCA000143945_00184" }, { "type": "cds", "sequence": "contig_1", "start": 100543, "stop": 101934, "strand": "+", "frame": 1, "gene": "lolD", "product": "Putative ABC transporter ATP-binding MG065-like protein", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75612", "UniRef:UniRef90_P75612" ], "nt": "ATGCGATACTTTGCCCTCACTGATTTAACCAAGCCCACTGCAAAATTTACCACTGATGCCATGGAACTGCGTCAGATATCCAATGCTTACCTCAACCAAGCCCAAGCATATTTGCAAAAGGGTTTAAAGCAACTCAAAAAGGATTACAAGAATGCCATTTTGTATACCCCCAAAACGGAGTACAAGCGCTTTTTGAAATGGAAGCAAACTTTTCTTCAAGATTTAAACCAAACCCAAAAGCGCTTCTTTATTGTTCGTGCCCAACATTTTTCTTACGTGTTGTTGTTTAACTTATTGGATGAACAGGTGGAAGCGGTGATTGCGACCTTTAACAACTTCTTGGATGAACACCGCTTGGAGGCACAATCCAAGCAGTTTGATTTAGACACAGCTGTCAATGAACTGCATGATTACTTTGACCAACTGCAAAAGAACACCGCTTACAGCGAGGATTTACCAACTCACCTCACGCAAAAAACCGAGCAATTAATTAACGCTCGTAATACCCAGTTAACGAATTTGTTAAACAAGATTGCTACTACCAAACCACCCCTAAACAAACAGCAACGTTTGTTAGCCAGTTTTCGCAATTACCACGAACACCTCTTTTTAAAGAACGAGGTCAAAAAGGTGACTTGGTTAAACGAACCACGCGCCAAAAAAGAGTCTGTTACCCCTGATGAAGAACACATTATTGAACTTAAAAACGTTTACAAGTACATTACTAATGGGGTGACTACCAATGCCGTTTTAAAGGGCATAGATCTCAAACTCAAAGCCCATGATTTCATTGTGATTTTAGGTCCCTCTGGCTCGGGGAAAACGACCCTGCTCAACATTATTTCCGGGATGGACCGGCCATCAAGCGGATCTGTTGTCGTTAATGGTCAGGAAATGATTTGCATGAACGACCGTCAGTTAACTAACTTTCGCCGCAACTATGTCGGTTACATCTTCCAGCAGTACGGGCTTTTACCCAACCTAACTGTAAGGGAAAACGTTGAAGTGGGTGCTAACTTGCAACGTAACCCTGATAAGCGGATTAACATTGATGAACTGTTAGAAGCAGTAGGGATGAAACACCTCCAAAAGAAACTCCCCAACGAGTTGAGTGGTGGACAACAACAACGGGTCTCGATTGCACGGGCGTTTGCTAAAAATCCCTTACTCATTTTTGGTGATGAACCAACTGGGGCTTTGGATTTGGAAATGACCCAAATTGTGCTCAAACAGTTTTTAGCCATTAAACAACGCTATAAAACCACGATGGTCATAGTGACGCACAATAACCTAATTGCCCAACTAGCCGATTTAGTCATTTATGTGGCAGATGGCAAAATTCAAGCACTGCAAGCAAACCCTAATCCCAAACAGGTTGAGGACATTAACTGA", "aa": "MRYFALTDLTKPTAKFTTDAMELRQISNAYLNQAQAYLQKGLKQLKKDYKNAILYTPKTEYKRFLKWKQTFLQDLNQTQKRFFIVRAQHFSYVLLFNLLDEQVEAVIATFNNFLDEHRLEAQSKQFDLDTAVNELHDYFDQLQKNTAYSEDLPTHLTQKTEQLINARNTQLTNLLNKIATTKPPLNKQQRLLASFRNYHEHLFLKNEVKKVTWLNEPRAKKESVTPDEEHIIELKNVYKYITNGVTTNAVLKGIDLKLKAHDFIVILGPSGSGKTTLLNIISGMDRPSSGSVVVNGQEMICMNDRQLTNFRRNYVGYIFQQYGLLPNLTVRENVEVGANLQRNPDKRINIDELLEAVGMKHLQKKLPNELSGGQQQRVSIARAFAKNPLLIFGDEPTGALDLEMTQIVLKQFLAIKQRYKTTMVIVTHNNLIAQLADLVIYVADGKIQALQANPNPKQVEDIN", "aa_hexdigest": "08a651a5d28d33956c8c41142d0fd463", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75612", "query_cov": 1.0, "subject_cov": 0.9956989247311828, "identity": 1.0, "score": 894.0, "evalue": 0.0, "valid": true, "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG065 homolog", "uniref50_id": "UniRef50_P75612", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75612", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75612" ], "product": "Putative ABC transporter ATP-binding protein MG065 homolog" }, "genes": [ "lolD" ], "id": "CNOCLNGIAL_224", "locus": "GCA000143945_00185" }, { "type": "cds", "sequence": "contig_1", "start": 101961, "stop": 102575, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "ATGAAAAACCTGTTTGCTTGCCAACACTTAGCGTTAAGTGCTATTCAACACGCTAAGGGTGGTCATGTGGGAATGGCCTTAGGTGCTAGTCCTATTTTGTACACCTTATGGACTAAACACATCCAGTTTAACCCTAACTGTCCCAAATGGATTAACCGGGACCGTTTAGTGATGAGTGCAGGTCATGGTAGTATGGCCTTATACCCGATCTTACACTTTGCGGGGTTAATTACGAAGCAGGAAATGCTCCATCACAAATACGGGCAAGTCAACACCTCTTCCCATCCCGAGTATGCCCCTAATAACTTTATTGATGCGTCCACTGGTCCCTTAGGACAAGGCCTAGGAATGGCTGTCGGGATGGCCTTGACACAACGGGTACTAGCAGCTGAATTTAAAGCGTTAAGTCCCAAACTGTTTGACCACTTTACCTATGTGGTTGTGGGTGATGGTGATCTCCAGGAAGGGGTGAGTTATGAGGTAGCTCACTTAGCAGGGGTCTACCAGTTAAATAAGCTAATAGTGCTCCATGATTCCAACCGGGTGCAGATGGACAGTGTCGTGCGTGATGTGTCCTTGGAAAACCTGCAAACCCGGTTTACCAACATGGGGTGA", "aa": "MKNLFACQHLALSAIQHAKGGHVGMALGASPILYTLWTKHIQFNPNCPKWINRDRLVMSAGHGSMALYPILHFAGLITKQEMLHHKYGQVNTSSHPEYAPNNFIDASTGPLGQGLGMAVGMALTQRVLAAEFKALSPKLFDHFTYVVVGDGDLQEGVSYEVAHLAGVYQLNKLIVLHDSNRVQMDSVVRDVSLENLQTRFTNMG", "aa_hexdigest": "2e6a12f53cfc188c3415e6b1af99b6bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 198, "aa_cov": 0.9656862745098039, "hmm_cov": 1.0, "evalue": 1.7859538388176003e-62, "score": 206.6949920654297, "start": 7, "stop": 203 } ], "seq_stats": { "molecular_weight": 22438.606300000003, "isoelectric_point": 7.886175727844237 }, "id": "CNOCLNGIAL_225", "locus": "GCA000143945_00186" }, { "type": "cds", "sequence": "contig_1", "start": 102663, "stop": 102917, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCACACGACAATTGCTAAAAACACCACCTTGGAAGACCAACCCGCTGGTCACTGGTTTATTCCTACCGACAAGGACTTTGCGCGCTTTAACAGCAACACCAAGACTAACTTTACCCCGTTTGAATATCCCCAAACGGTGTATGACTTCTTTCACAAGCAGGTCATAGCACGCCAAGCTAAACCAGTGCAAGCTTACAAGGAATTACTGGAAAAACTAAAGGACAAACCGCTGTATACTAAGTTTATTAATTGA", "aa": "MHTTIAKNTTLEDQPAGHWFIPTDKDFARFNSNTKTNFTPFEYPQTVYDFFHKQVIARQAKPVQAYKELLEKLKDKPLYTKFIN", "aa_hexdigest": "8e35bb182d4d48773d49830a5ca94b70", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9954.206299999994, "isoelectric_point": 9.222992134094238 }, "id": "CNOCLNGIAL_226", "locus": "GCA000143945_00187" }, { "type": "cds", "sequence": "contig_1", "start": 102939, "stop": 103391, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02779.29" ], "nt": "TTGTACCTCAACCAGTTAGATGAACGCAAGGTAGCACAAACCAATGCGGCAACCCGTAACTACCTCAAGGACTTTTTAGGCCAAATTAACAACAGTAATTCCAACTTGTACTGTCTCAATGCTGATGTAGCACGTTCGTGTAACATTAAGTTAGGTGATGATAATTTACATACCAATCCGCATTCGCGCAACATTCAGGTGGGTATTCGGGAATTTGGTATGTCCACCATTATGAACGGGATGGCACTCCATGGTGGGGTCAAAGTGATGGGTGGTACATTCCTAGCGTTTGCTGATTATTCCAAACCCGCAATTCGCTTAGGGGCGTTAATGAACCTCCCCACCTTTTATGTGTACACCCATGACTCTTACCAAGTCGGTGGTGATGGACCAACCCACCAACCGTACGATCAACTCCCGATGTTAAGGGCCATTGAAAACGTTCAGGTTTGA", "aa": "MYLNQLDERKVAQTNAATRNYLKDFLGQINNSNSNLYCLNADVARSCNIKLGDDNLHTNPHSRNIQVGIREFGMSTIMNGMALHGGVKVMGGTFLAFADYSKPAIRLGALMNLPTFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVQV", "aa_hexdigest": "41ea7780e1f6ce05a0b2adb71f09525b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02779.29", "name": "Transket_pyr", "length": 137, "aa_cov": 0.9, "hmm_cov": 0.9927007299270073, "evalue": 6.02322890881346e-36, "score": 119.15585327148438, "start": 16, "stop": 150 } ], "seq_stats": { "molecular_weight": 16669.72029999999, "isoelectric_point": 6.952681922912597 }, "id": "CNOCLNGIAL_227", "locus": "GCA000143945_00188" }, { "type": "cds", "sequence": "contig_1", "start": 103437, "stop": 103907, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22613.1" ], "nt": "TTGTTAAGCCAAGACCAAACTAACGTATTAATTTTGACCCGTCAAGCTTTGCCTTCCCTAGAACAATCTGATAGTGTACAGACCTTGAAAGGGGGCTATATTATTAGTAATAGAAAACAACCCGATGTCATTGTGGCCGCTAGTGGCAGTGAAGTTCAGCTGGCATTGCAACTAGAACAAGCTCTAAACGAACAACAATTAAAGACGCGGGTGGTGTCAGTGCCCAACATTAACATGTTGTTAAGTCAACCCCAAAGCTACTTGCAACAACTGTTTGATCCTAACAGTGTTTTACTCACCTTAGAAGCGAGTGCTAGCATGGAGTGGTATGCACTAGCAAAATACGTCAAAAAGCACACCCACTTAGGTGCTTTTAGCTTTGGTGAGTCCAATGACGGGCAAGTCGTGTATGAACACAAGGGCTTTAATGTGACTAACTTACTCAAGTTGATTAAAACTCTAAAGAGTTAA", "aa": "MLSQDQTNVLILTRQALPSLEQSDSVQTLKGGYIISNRKQPDVIVAASGSEVQLALQLEQALNEQQLKTRVVSVPNINMLLSQPQSYLQQLFDPNSVLLTLEASASMEWYALAKYVKKHTHLGAFSFGESNDGQVVYEHKGFNVTNLLKLIKTLKS", "aa_hexdigest": "5a3123250bc381e5427d9d4d9ec847a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22613.1", "name": "Transketolase_C_1", "length": 115, "aa_cov": 0.7243589743589743, "hmm_cov": 0.9826086956521739, "evalue": 4.110762222463199e-28, "score": 93.36075592041016, "start": 31, "stop": 143 } ], "seq_stats": { "molecular_weight": 17330.6251, "isoelectric_point": 6.829852867126465 }, "id": "CNOCLNGIAL_228", "locus": "GCA000143945_00189" }, { "type": "cds", "sequence": "contig_1", "start": 104463, "stop": 104591, "strand": "+", "frame": 3, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F4E8A2E", "UniRef:UniRef90_UPI001F4E8A2E" ], "nt": "GTGATTGAGCAGAAGCTCAAGGAAGATTCGCTGTTGTTTATCCAAACGAGTACGTTACCTAAAACAGCATACGCAGCAATTGATCCGGTGAACTTTAGCTATAACCCAACCCGTACCAACGGTTTTTGA", "aa": "MIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGF", "aa_hexdigest": "2f1356b19045b9a466b124ba6dfd607d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 104347, "stop": 104602, "observations": { "deletion": [ 104380, 104587 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F4E8A2E", "query_cov": 1.0, "subject_cov": 0.49411764705882355, "identity": 0.976, "score": 85.5, "evalue": 6.18e-21, "gene_start": 1, "gene_end": 42, "reference_start": 40, "reference_end": 81, "reference_sequence": "MVGVNHILTSKKTQSFLLGKYTKPNVQLVKTNYEKPEDAVIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGFLNSR" }, "paralog": false, "identity": 1.0, "score": 160.0, "evalue": 8.25e-53, "description": "Frameshift due to deletion around 104380, 104587.", "psc": { "uniref90_id": "UniRef90_UPI001F4E8A2E", "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_UPI001F4E8A2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F4E8A2E", "UniRef:UniRef50_UPI001F4E8A2E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F4E8A2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F4E8A2E" ], "product": "DUF31 family putative serine protease" } }, "id": "CNOCLNGIAL_229", "locus": "GCA000143945_00190" }, { "type": "cds", "sequence": "contig_1", "start": 104655, "stop": 105179, "strand": "+", "frame": 3, "gene": null, "product": "DUF31 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AB22BFD", "UniRef:UniRef90_UPI000AB22BFD" ], "nt": "GTGATTGAAGTACCGTTAGTACTATCCAACCCCAATGATGCCAAGATCTACCAAGAGTGGATTAGACCAGCTACCCAAGCCTACAAATATTTAGGTGATGTAGAAGGTTTGTTTGCTAAGAAGGGCTATCGCAGTTACATCCAAGATTTCTACCACTTATTGGGTTATCCGGTAACTAAGAACACTAAATCAGAGTTTATTTTGGGACAAAGTCAAGGTACTGTGAACCACATGAGTTTTAGTAATGATGAGTCCAATACTACTAATACTATTACTAAAGCTAGCTTAACTCAGCAACCCCACAAGGAACAATCAGCTTACGTGGTACGTGAAAGTGGACTGCCAACCCTAACAATGAATGTGGACAAGTACACTGGTGCAAAGGGAACTCACCTAGTTAACGTTGACCAAATTACTGACCTTAGTTTGGGTGATGGTTTAATTGACTTTGGTGGATTATCACGCTTTATCTTGCAGTACCACAATGTGAACTATAAGCAATTTGGTTATGGCACGATCTTGTGA", "aa": "MIEVPLVLSNPNDAKIYQEWIRPATQAYKYLGDVEGLFAKKGYRSYIQDFYHLLGYPVTKNTKSEFILGQSQGTVNHMSFSNDESNTTNTITKASLTQQPHKEQSAYVVRESGLPTLTMNVDKYTGAKGTHLVNVDQITDLSLGDGLIDFGGLSRFILQYHNVNYKQFGYGTIL", "aa_hexdigest": "3cb5f5faca0d6a4e7f2e23a9e0102508", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 104357, "stop": 105480, "observations": { "insertion": [ 104381, 105420 ], "stop": [ 104589, 105177 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AB22BFD", "query_cov": 1.0, "subject_cov": 0.4496124031007752, "identity": 0.9940000000000001, "score": 349.0, "evalue": 1.4e-118, "gene_start": 1, "gene_end": 174, "reference_start": 101, "reference_end": 274, "reference_sequence": "MGSTISLPATQSFLLGKYTKPNVQLVKTNYEKPEDAVIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGFWTAGKYNVYNGGNSIGNYADFAVIEVPLVLSNPNDAKIYQEWIRPATQAYKYLGDVEGLFAKKGYRSYIQDFYHLLGYPVTKNTKSEFILGQSQGTVNHMSFSNDESNTTNTITKASLTQQPHKEQSAYVVRESGLPTLTMNVDKYTGAKGTHLVNVDQITDLSLGDGLIDFGGLSRFILQYHNVNYKQFGYGTILWDTNFAGGSSGSAIFNQNKQINSIYFGALVNVTTDRNENVGLGLGQILRAPNTFNSSHEVPYSYDLIFGDVNTTNFYAQFAKKHNTHMWSKIQSTQNGEIGFHKNSKTGQQRH" }, "paralog": false, "identity": 0.9919571045576407, "score": 731.0, "evalue": 1.63e-271, "description": "Internal stop codon at 104589, 105177. Frameshift due to insertion around 104381, 105420.", "psc": { "uniref90_id": "UniRef90_UPI000AB22BFD", "product": "DUF31 family protein", "uniref50_id": "UniRef50_UPI000AB22BFD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AB22BFD", "UniRef:UniRef50_UPI000AB22BFD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AB22BFD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AB22BFD" ], "product": "DUF31 family protein" } }, "id": "CNOCLNGIAL_230", "locus": "GCA000143945_00191" }, { "type": "cds", "sequence": "contig_1", "start": 105294, "stop": 105530, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTTTAGGCTTAGGGCAAATTCTCCGTGCTCCCAATACCTTCAACTCTTCCCACGAAGTGCCCTATTCTTATGACCTCATCTTTGGTGATGTTAACACCACCAATTTTTACGCCCAGTTTGCGAAAAAAGCACAACACCCACATGTGGAGCAAAATTCAAAGTACGCAGAATGGAGAAATTGGTTTCCACAAAAATTCAAAAACCGGTCAACAGCGGCATTAATCGGTTTTTGA", "aa": "MGLGLGQILRAPNTFNSSHEVPYSYDLIFGDVNTTNFYAQFAKKAQHPHVEQNSKYAEWRNWFPQKFKNRSTAALIGF", "aa_hexdigest": "7ad073681ce2c3ed1008358aefa926ef", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8995.976499999997, "isoelectric_point": 9.454820442199708 }, "id": "CNOCLNGIAL_231", "locus": "GCA000143945_00192" }, { "type": "cds", "sequence": "contig_1", "start": 105569, "stop": 106111, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGTTACTCCGCTCTGTTTGGTATAAGCTTGGTAGTCTGTTAATCATCTTACCGCTAACGGGTTGTGGTTATGTCAGACTAAAGCGTGCTAATTTCCAAACAGACTTTACCATTAACCGCATTCCCACCCAAGGGGATGTGTACCATGATAACTATGACCTGACTTTTTCACTCAACTTTGCTACTAGTTCCAAAGATTCTTATGGTACGGGTTGGTTAATTGACTGGAAGGGTGATGAGAATAAGCCTAGCAATACCGATCCCTTCCTGATTTACTTAGCGACTAACCTACACGTAGTCGATGCCTTGCGCAATCCCCAAGACTATGAACCTTACAATAAGGATAGTAATGGTCAGGACTATGGTAATCGTGATATAACCCATTTCTTTGCATTGGGTAAATACACTGATCCAGGTTTATTGGGTGTTGAAACAAAAGAGCAATCAGCCTTTATTTCGATTCAAACATCAGCAATCCCCAAAACCGCTTATACGGCTAATGATTTTGTTGATTATCAGTATGATAGTCTTACAAAACAGTGA", "aa": "MLLRSVWYKLGSLLIILPLTGCGYVRLKRANFQTDFTINRIPTQGDVYHDNYDLTFSLNFATSSKDSYGTGWLIDWKGDENKPSNTDPFLIYLATNLHVVDALRNPQDYEPYNKDSNGQDYGNRDITHFFALGKYTDPGLLGVETKEQSAFISIQTSAIPKTAYTANDFVDYQYDSLTKQ", "aa_hexdigest": "adadb7b863850d210c0b140423272ece", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 148, "aa_cov": 0.7277777777777777, "hmm_cov": 0.9391891891891891, "evalue": 5.879149734961855e-37, "score": 123.53508758544922, "start": 46, "stop": 176 } ], "seq_stats": { "molecular_weight": 20493.577099999988, "isoelectric_point": 5.059659385681152 }, "id": "CNOCLNGIAL_232", "locus": "GCA000143945_00193" }, { "type": "cds", "sequence": "contig_1", "start": 106211, "stop": 107143, "strand": "+", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_084", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47314", "UniRef:UniRef90_P75609" ], "nt": "GTGCCATTGTTCTTAGACAACGTCCGTGACAAACAGGTCTTTGATTATTTTGTCAAACCAGCCATTGAGACCTATAAAAAGCTTGGTGATACGGCTCAATTATTTACTGAGCAAAACCTAAAGGAATTGGAAAAGGAAACCTATATTATGCTGGGCTATCCGGTAGTGGAGAGCAATATTTATGCCCACATTTTAGGTCAAGGAAAAGAGTTGCGTGTGACTCAACAAGTCAACAACCAACCAGTTAAAAAGAACTATGTTTTGCAAACCATTACTAAAGAGCAACACACTTTGGATATTACGCGTGAAATACCAACCTTAATCCAAAACAAGAGTTTATCGGGTGATTTTGTTGGTAGTAAGCTCTTATCTCAAGAAGAACAACAGCAAGAACATGCTTTTTTGGGTAACTTAAACCAAGGGATAATTGACTTTGCTAGATTGTCAAACTTTAACTTGCAGTACCACAACCGCGAATACCAACAATACGGTAAGGGTTTAGCATTAGCTAACACTAACTTTAGTGGTGGTTCATCCGGTACCTTAGTTTTAAATCAACAAAAACAAATTTCCGGGGTTTACTTTGGCGTGTTAGAGTTTGGTGGCACCAACGGCACTAACCGTGAAAGTAGTATTGGTGTTGGTCAAATTTTGCGAGTAAAAGATGACGCTCAACTCCAACAACACAGTCACAACAACTTACTTTCACAATTAGGTTCCAGTCATAACTCGATTACTTACGACATTATTTTTGGTAACAAAGACACTAAAAACTACTACGCCCAGTTTGCCAAAAAACATCAAACACATTTGTACAGCCAAATAAGTTCAAGTAATCAGCAAGAACTCAAATACGTCGATAACGATCCAAATCTCAAGATTAAAGAAGAGGCTGCCAAAAGCACTGTTCAGAATTTAACTGTCTACAGTTAA", "aa": "MPLFLDNVRDKQVFDYFVKPAIETYKKLGDTAQLFTEQNLKELEKETYIMLGYPVVESNIYAHILGQGKELRVTQQVNNQPVKKNYVLQTITKEQHTLDITREIPTLIQNKSLSGDFVGSKLLSQEEQQQEHAFLGNLNQGIIDFARLSNFNLQYHNREYQQYGKGLALANTNFSGGSSGTLVLNQQKQISGVYFGVLEFGGTNGTNRESSIGVGQILRVKDDAQLQQHSHNNLLSQLGSSHNSITYDIIFGNKDTKNYYAQFAKKHQTHLYSQISSSNQQELKYVDNDPNLKIKEEAAKSTVQNLTVYS", "aa_hexdigest": "0b1614891baa0c2f003b77c7375ee4f0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 105911, "stop": 107143, "observations": { "mutation": [ 106109, 106169 ], "stop": [ 106109, 106169 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75609", "query_cov": 1.0, "subject_cov": 0.5893536121673004, "identity": 0.997, "score": 600.0, "evalue": 2.31e-213, "gene_start": 1, "gene_end": 310, "reference_start": 217, "reference_end": 526, "reference_sequence": "MPCLLRSVWYKLGSLLIILPLTGCGYVRLKRANFQTDFTINRIPTQGDVYHDNYDLTFSLNFATSSKDSYGTGWLIDWKGDENKPSNTDPFLIYLATNLHVVDALRNPQDYEPYNKDSNGQDYGNRDITHFFALGKYTDPGLLGVETKEQSAFISIQTSAIPKTAYTANDFVDYQYDSLTKQWNKKSDQKDQTQEQTVTGQSWSYKPAYADFAVIEVPLFLDNVRDKQVFDYFVKPAIETYKKLGDTAQLFTEQNLKELEKETYIMLGYPVVESNIYAHILGQGKELRVTQQVNNQPVKKNYVLQTITKEQHTLDITREIPTLIQNKSLSGDFVGSKLLSQEEQQQEHAFLGNLNQGIIDFARLSNFNLQYHNREYQQYGKGLALANTNFSGGSSGTLVLNQQKQISGVYFGVLEFGGTNGTNRESSIGVGQILRVKDDAQLQQHSHNNLLSQLGSSHNSITYDIIFGNKDTKNYYAQFAKKHQTHLYSQISSSNQQELKYVDNDPNLKIKEEAAKSTVQNLTVYS" }, "paralog": false, "identity": 0.9951219512195122, "score": 810.0, "evalue": 3.96e-298, "description": "Internal stop codon at 106109, 106169. Nonsense mutation around 106109, 106169.", "psc": { "uniref90_id": "UniRef90_P75609", "product": "Uncharacterized lipoprotein MPN_084", "uniref50_id": "UniRef50_P47314", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75609", "UniRef:UniRef50_P47314", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47314", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47314" ], "product": "Uncharacterized lipoprotein MG068" } }, "id": "CNOCLNGIAL_233", "locus": "GCA000143945_00194" }, { "type": "cds", "sequence": "contig_1", "start": 108225, "stop": 108542, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTAATATGGGAGAGCAATCATCCAAAAACATGAATTCTTTTGAAAATCATGAATCAAATGAACGAAATAGTCTTCAAATTTATCAACAACATTCATCTATGATGTCAGAAACAAAGAGAAAACAAAGTAGCTTAAGTTATGATGCCAGAATTCTTTTACCTAAAAGTCCCTATAACACAAAGAAAACTTTATTTTTAATTATTTTCTTTTCCATTATCTCCTTGATATTGGCAACTATAGGTAGTGTGTTCATTTCTTTTGCTATAGTTCAAATTTCTATACCTTTTTACGTTATAGGTGGAGTTATTTGA", "aa": "MNINMGEQSSKNMNSFENHESNERNSLQIYQQHSSMMSETKRKQSSLSYDARILLPKSPYNTKKTLFLIIFFSIISLILATIGSVFISFAIVQISIPFYVIGGVI", "aa_hexdigest": "c7364aee15d1e5ef69f7ea04bbb26428", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11927.690599999985, "isoelectric_point": 9.397250175476074 }, "id": "CNOCLNGIAL_234", "locus": "GCA000143945_00195" }, { "type": "cds", "sequence": "contig_1", "start": 108829, "stop": 109038, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_086", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75607", "UniRef:UniRef90_P75607" ], "nt": "ATGGAGTCTGCACTAAACCAAGAGTTTCAAATTGATTTTTGTGTAAAAAACAAAAAACTGTTAAAAATTTTAGCTAACGTTCTTATTGCTTCTTGGTTATCGTTTGTTGTTTTTTTAATCCTAGGTTGTATAGCTATTGATCTTTTTCGATTTGATCTTTATTCCCAATTTTTCTTTAATCACTTAAGTACTTTATCTGCTTTAGCATGA", "aa": "MESALNQEFQIDFCVKNKKLLKILANVLIASWLSFVVFLILGCIAIDLFRFDLYSQFFFNHLSTLSALA", "aa_hexdigest": "1343b7ae5fddda2403c543ec02b2302e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 108829, "stop": 109146, "observations": { "mutation": [ 109036 ], "stop": [ 109036 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75607", "query_cov": 1.0, "subject_cov": 0.6571428571428571, "identity": 1.0, "score": 131.0, "evalue": 1.77e-38, "gene_start": 1, "gene_end": 69, "reference_start": 1, "reference_end": 69, "reference_sequence": "MESALNQEFQIDFCVKNKKLLKILANVLIASWLSFVVFLILGCIAIDLFRFDLYSQFFFNHLSTLSALAWTFFVLAILFGAATLAINGFFYKEIRKKSAFKEDSK" }, "paralog": false, "identity": 0.9904761904761905, "score": 202.0, "evalue": 5.79e-69, "description": "Internal stop codon at 109036. Nonsense mutation around 109036.", "psc": { "uniref90_id": "UniRef90_P75607", "product": "Uncharacterized protein MPN_086", "uniref50_id": "UniRef50_P75607", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75607", "UniRef:UniRef50_P75607", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75607" ], "product": "Uncharacterized protein MPN_086" } }, "id": "CNOCLNGIAL_235", "locus": "GCA000143945_00196" }, { "type": "cds", "sequence": "contig_1", "start": 109818, "stop": 110105, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532737.1", "SO:0001217", "UniParc:UPI0003669FDE", "UniRef:UniRef100_A0AAV5N6K4", "UniRef:UniRef50_A0AAV5N6K4", "UniRef:UniRef90_A0AAV5N6K4" ], "nt": "ATGGAAAAAGAAACAAAGAAAGATTTATTAAGTGAAGTGAAAAATAGCTTTCATTCTTCTAACAAAAATATTAAAAGCGAAATTATTTTGAAGAACACTTCAGAAAATAATATTTATTTAAGAAGCGATGCGAGAAGGCTTTTACCTAGAATCCCTTATAACACAAAGAGTGCATTATTATTCACCCTTTTATTTGCATTTCTATCTGGCCTTTCTTATGGTCTTGCATCTTTATGTATTAACTTCTCGTTTACTTCGCCACAAATTTTCATTGTCTTAATTTATTAG", "aa": "MEKETKKDLLSEVKNSFHSSNKNIKSEIILKNTSENNIYLRSDARRLLPRIPYNTKSALLFTLLFAFLSGLSYGLASLCINFSFTSPQIFIVLIY", "aa_hexdigest": "c31071c134f3730ab7ce49904dec96ae", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003669FDE", "ncbi_nrp_id": "WP_017532737.1", "uniref100_id": "UniRef100_A0AAV5N6K4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003669FDE", "RefSeq:WP_017532737.1", "UniRef:UniRef100_A0AAV5N6K4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N6K4", "uniref90_id": "UniRef90_A0AAV5N6K4" }, "psc": { "uniref90_id": "UniRef90_A0AAV5N6K4", "uniref50_id": "UniRef50_A0AAV5N6K4" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N6K4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N6K4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10880.54749999999, "isoelectric_point": 9.561386680603025 }, "id": "CNOCLNGIAL_236", "locus": "GCA000143945_00197" }, { "type": "cds", "sequence": "contig_1", "start": 110358, "stop": 110813, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532738.1", "SO:0001217", "UniParc:UPI00037AA697", "UniRef:UniRef100_A0AAX0S4M2", "UniRef:UniRef50_A0AAX0S4M2", "UniRef:UniRef90_A0AAX0S4M2" ], "nt": "GTGAAACAGAATTTAAGTAGTAGAGACTTTATTAAAAAGTCAGAAAAAACAGTATCAACTACTGTAAATATAAATACAGAAGCTAGTAGTTATATTGCCAAAAATCTAGAAAACCAAAAGGCAGTTCTTTCAGCAAAGGAATTAGAAAAACAAGAGCTTTTACTAGTGCGCTTAAATGCAGTAAAAAGATTTCCGTGCTATCCATACAATACAAAAAAGGCATTTAATATTTCTGTTTTATTTGTTCTATTAGCTGGTCTATTTTTTACTGCGTCTTATTTCTCTATTTTTTATTCGATTTTTACTAGGTTTAAAGCTTTACTTATTTTCTTAGGAGTAGTTTTTATTCTTATAACTTTCATTATGCTAATTCATTGCTATATATGGTCAGTAAGGTTCAAATCAGAGTACCTTGCTAATCGTATTTATGCACTGAATTTTCAAAAAGGAGCCTAA", "aa": "MKQNLSSRDFIKKSEKTVSTTVNINTEASSYIAKNLENQKAVLSAKELEKQELLLVRLNAVKRFPCYPYNTKKAFNISVLFVLLAGLFFTASYFSIFYSIFTRFKALLIFLGVVFILITFIMLIHCYIWSVRFKSEYLANRIYALNFQKGA", "aa_hexdigest": "494e3fbfd8244fffa4a5a0f0feff0929", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00037AA697", "ncbi_nrp_id": "WP_017532738.1", "uniref100_id": "UniRef100_A0AAX0S4M2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00037AA697", "RefSeq:WP_017532738.1", "UniRef:UniRef100_A0AAX0S4M2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S4M2", "uniref90_id": "UniRef90_A0AAX0S4M2" }, "psc": { "uniref90_id": "UniRef90_A0AAX0S4M2", "uniref50_id": "UniRef50_A0AAX0S4M2" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0S4M2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S4M2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17498.497899999988, "isoelectric_point": 9.856780815124512 }, "id": "CNOCLNGIAL_237", "locus": "GCA000143945_00198" }, { "type": "cds", "sequence": "contig_1", "start": 110863, "stop": 111090, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_088", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64A6", "UniRef:UniRef100_P75605", "UniRef:UniRef50_P75605", "UniRef:UniRef90_P75605" ], "nt": "ATGATAGAAATAAATAATTTGACCGCTTTAGAAAGGGATGAAAAAGTAGAAACGGAAGACATTAATACCAATGTAATAGCAAATACTTATACAGTGCAAGCCAATGTTCCAACTGCATTTCAAACCAGTGAAACAAATAGTAATACTGTTACTAACGTCCCAAAATCTCCAGAGTTAATAAGTATTCAAAGATTAGAAAGATTAAAAAATAGGTATACTTTCCTATAA", "aa": "MIEINNLTALERDEKVETEDINTNVIANTYTVQANVPTAFQTSETNSNTVTNVPKSPELISIQRLERLKNRYTFL", "aa_hexdigest": "63509dd9769b595e72105a39ef4b8b97", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64A6", "uniref100_id": "UniRef100_P75605", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64A6", "UniRef:UniRef100_P75605" ] }, "ips": { "uniref100_id": "UniRef100_P75605", "uniref90_id": "UniRef90_P75605" }, "psc": { "uniref90_id": "UniRef90_P75605", "product": "Uncharacterized protein MPN_088", "uniref50_id": "UniRef50_P75605" }, "pscc": { "uniref50_id": "UniRef50_P75605", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75605" ], "product": "Uncharacterized protein MPN_088" }, "genes": [], "id": "CNOCLNGIAL_238", "locus": "GCA000143945_00199" }, { "type": "cds", "sequence": "contig_1", "start": 111450, "stop": 111629, "strand": "+", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD5284", "UniRef:UniRef90_UPI0019169453" ], "nt": "GTGGAGATTAAAACTTACAAAATTAAAGATATTTGTGAAGCTTCACATGGAAGAGAAATAAATACTAAATATCTCCGTGAAAATCAAGGAATTTATCCTGTTTATTCATCAGCAACTTCTAATGAAGGTGAAATGGGCAGAATCAAAACTTACGATTTTGATGGTGAATATGTTACCTGA", "aa": "MEIKTYKIKDICEASHGREINTKYLRENQGIYPVYSSATSNEGEMGRIKTYDFDGEYVT", "aa_hexdigest": "fad4a179cb312d5d5e4ee0fcbc69cd9b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 111450, "stop": 111686, "observations": { "mutation": [ 111627, 111639 ], "stop": [ 111627, 111639 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019169453", "query_cov": 1.0, "subject_cov": 0.7468354430379747, "identity": 1.0, "score": 127.0, "evalue": 3.64e-37, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MEIKTYKIKDICEASHGREINTKYLRENQGIYPVYSSATSNEGEMGRIKTYDFDGEYVTWTTRWSYAGSIYYRNGKFSG" }, "paralog": false, "identity": 0.9615384615384616, "score": 155.0, "evalue": 1.09e-50, "description": "Internal stop codon at 111627, 111639. Nonsense mutation around 111627, 111639.", "psc": { "uniref90_id": "UniRef90_UPI0019169453", "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI0034DD5284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019169453", "UniRef:UniRef50_UPI0034DD5284" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034DD5284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD5284" ], "product": "restriction endonuclease subunit S" } }, "id": "CNOCLNGIAL_239", "locus": "GCA000143945_00200" }, { "type": "cds", "sequence": "contig_1", "start": 111571, "stop": 111708, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGTGAAATGGGCAGAATCAAAACTTACGATTTTGATGGTGAATATGTTACCTGAACTACACGTTGAAGTTATGCCGGTAGCATTTACTATCGAAATGGTAAATTTAGTGCTTCCTCAAATTGTGGAATTTTAA", "aa": "MKVKWAESKLTILMVNMLPELHVEVMPVAFTIEMVNLVLPQIVEF", "aa_hexdigest": "8d282147aec9d86659585dac6a39468c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5212.366299999999, "isoelectric_point": 5.012824058532714 }, "id": "CNOCLNGIAL_240", "locus": "GCA000143945_00201" }, { "type": "cds", "sequence": "contig_1", "start": 111849, "stop": 112019, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAATTCAAGAAAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATTTACTTAACCCAAAAAATTGA", "aa": "MQIQEKIATILDTFTELSAELSAELSAELSAELSAELRERKKQYAFYRDYLLNPKN", "aa_hexdigest": "e9cd96aaa807178d9691539373410bf0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6481.2543000000005, "isoelectric_point": 4.761823081970214 }, "id": "CNOCLNGIAL_241", "locus": "GCA000143945_00202" }, { "type": "cds", "sequence": "contig_1", "start": 112164, "stop": 112556, "strand": "+", "frame": 3, "gene": null, "product": "Putative type I specificity subunit S-MpnORF289P C-terminus", "db_xrefs": [ "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75487", "UniRef:UniRef90_P75487" ], "nt": "TTGGTTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGCCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTACCTAAAGAAGAATTTGATATAAGGTTTTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCTCAATTTAAGGAGTATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCTGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTATTCCTGCTGAAATTGAACTGTGCAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MVYSKTFRVEEKSITVSARGTIGAVFYRDFAYLPAVSLICFVPKEEFDIRFLFHALRAIKFKKQGSATGQLTVAQFKEYGIHVPSLKKQKEIAAILDPLYSFFTDLNEGIPAEIELCKKQLDYYQNFLFN", "aa_hexdigest": "978c860a9603f0d19093b37a587f3b67", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75487", "query_cov": 1.0, "subject_cov": 0.896551724137931, "identity": 0.977, "score": 254.0, "evalue": 2.61e-85, "valid": true, "product": "Putative type I specificity subunit S.MpnORF289P C-terminus", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75487", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75487", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75487" ], "product": "Putative type I specificity subunit S.MpnORF289P C-terminus" }, "genes": [], "id": "CNOCLNGIAL_242", "locus": "GCA000143945_00203" }, { "type": "cds", "sequence": "contig_1", "start": 113719, "stop": 113841, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7C9", "UniRef:UniRef90_A0AB38W7C9" ], "nt": "ATGGGCGGCGCGCACAACATCCAGCCCCCCATTGAGCGGTGTCGTAAAGGGCGTGTAGGGGGTGAGGGTGTTGTGGTCTTGGAGGAGGTAGGTGTTGGAAACTATTTATTAATTTTTAAGTAA", "aa": "MGGAHNIQPPIERCRKGRVGGEGVVVLEEVGVGNYLLIFK", "aa_hexdigest": "f7368d3308c1261e8c6603226c024eca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W7C9", "query_cov": 0.925, "subject_cov": 0.925, "identity": 0.919, "score": 73.2, "evalue": 1.43e-16, "valid": true, "uniref50_id": "UniRef50_A0AB38W7C9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7C9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7C9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4292.982899999999, "isoelectric_point": 7.9588960647583 }, "id": "CNOCLNGIAL_243", "locus": "GCA000143945_00204" }, { "type": "cds", "sequence": "contig_1", "start": 113875, "stop": 114333, "strand": "+", "frame": 1, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_A0AB38W808" ], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGA", "aa": "MGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESG", "aa_hexdigest": "624c797a5e6239e80827d7a7c87242db", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 113827, "stop": 114540, "observations": { "mutation": [ 113869, 114331, 114439 ], "stop": [ 113869, 114331, 114439 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W808", "query_cov": 1.0, "subject_cov": 0.6413502109704642, "identity": 0.993, "score": 288.0, "evalue": 2.83e-97, "gene_start": 1, "gene_end": 152, "reference_start": 17, "reference_end": 168, "reference_sequence": "MRAAHLHTSYELVDWKVGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESGWDNQRSQKNLTNNPGPKAVTGFKLDKGRAYRKLNESWPVSNPSIRPRLARGKVPQNEKRRRQMLRVMLP" }, "paralog": false, "identity": 0.9831223628691983, "score": 455.0, "evalue": 2.21e-165, "description": "Internal stop codon at 113869, 114331, 114439. Nonsense mutation around 113869, 114331, 114439.", "psc": { "uniref90_id": "UniRef90_A0AB38W808", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W808", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "CNOCLNGIAL_244", "locus": "GCA000143945_00205" }, { "type": "cds", "sequence": "contig_1", "start": 114555, "stop": 114752, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGGCTGGAAGTGCTTTTGGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAGACCGCTCTTGAAGCACACCGCCCAAATTAATTTAAGGCAAACCCAAGACAACGCGCAAACTGGTAAATTCTCCAAATACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MGAGSAFGLQGNGSNSSGLRPLLKHTAQINLRQTQDNAQTGKFSKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "9672aa81bacb828e10d4ef5ef14e6332", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6855.643300000002, "isoelectric_point": 9.992099952697757 }, "id": "CNOCLNGIAL_245", "locus": "GCA000143945_00206" }, { "type": "cds", "sequence": "contig_1", "start": 114793, "stop": 114993, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "GTGGTGTTTCAGTCCAAGCAGCGACTCGGCTCGACTTCTACAAATGAAGATCTGCCGAATGTAATAACAAAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTTGTATTGGGTTGTCGGGGAGGACCAGGAATCGGGTCGCGCAACCTGA", "aa": "MVFQSKQRLGSTSTNEDLPNVITKLYHTSTSQLAYLNGQIVVMGSNAVPSLLYWVVGEDQESGRAT", "aa_hexdigest": "61d91db0556db3fcd3f4778337df6726", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 24, "aa_cov": 0.36363636363636365, "hmm_cov": 1.0, "evalue": 3.551932153099348e-09, "score": 31.76915740966797, "start": 43, "stop": 66 } ], "seq_stats": { "molecular_weight": 7232.0369, "isoelectric_point": 5.53852787017822 }, "id": "CNOCLNGIAL_246", "locus": "GCA000143945_00207" }, { "type": "cds", "sequence": "contig_1", "start": 115054, "stop": 115371, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F374488" ], "nt": "TTGGGTTTTAAAGATGACTCCAATTCGGATTCCAAGAATTCGAATTTGAAGGCTCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGAGCACGGAAAACAGTGCTGCCACCAAAGACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGCTCAACGGTCCGATTAATGGCTTGTTTACCCTGCTCGACAGCTTTGCCTATGTGA", "aa": "MGFKDDSNSDSKNSNLKAQGLTQPAYLIAGLDVVADHLVFAAFKAGAVGYDMSTENSAATKDQALAWSTTAGLDSAGGYKALVENTAGSTVRLMACLPCSTALPM", "aa_hexdigest": "117a739795def962c550dafa0f8bec37", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F3174D6", "query_cov": 0.8380952380952381, "subject_cov": 0.8712871287128713, "identity": 0.8859999999999999, "score": 151.0, "evalue": 1.16e-45, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI001F374488" }, "pscc": { "uniref50_id": "UniRef50_UPI001F374488", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F374488" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_247", "locus": "GCA000143945_00208" }, { "type": "cds", "sequence": "contig_1", "start": 115338, "stop": 115691, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0", "UniRef:UniRef90_UPI001F305329" ], "nt": "TTGTTTACCCTGCTCGACAGCTTTGCCTATGTGACCCCGGTGAGTGGCATGAAAGGGGGTAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGCGCGCTTGCCGTCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAATCAACTTCCGACGCTAATTCGAACACCAAAGTCAAGTGA", "aa": "MFTLLDSFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLNEARLPSRTDQLLVYGIVNESELKSARENAQSTSDANSNTKVK", "aa_hexdigest": "201987779b49ffe3547990682ad1dc22", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F305329", "query_cov": 0.8803418803418803, "subject_cov": 0.9035087719298246, "identity": 0.961, "score": 194.0, "evalue": 2.38e-62, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_A0AB38W8G0" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_248", "locus": "GCA000143945_00209" }, { "type": "cds", "sequence": "contig_1", "start": 116425, "stop": 116679, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAACACAGAATACGTAACGCGTAAAGAGTTTAATGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGAAAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGTTTGAACAAGATGGATGTTCGTCTAGATAAACTAGAGTCTAAGTAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLTKIETTLAAQGEQIRIQGEQIKELQIEQKAQGKTLKLILQTLQKMSDRLNKMDVRLDKLESK", "aa_hexdigest": "e9c72dd93cd27b9025b69c313a390df8", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.9642857142857143, "hmm_cov": 1.0, "evalue": 1.3514325516917017e-26, "score": 88.55352783203125, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 9913.243099999994, "isoelectric_point": 8.924567985534669 }, "id": "CNOCLNGIAL_249", "locus": "GCA000143945_00210" }, { "type": "cds", "sequence": "contig_1", "start": 116887, "stop": 117135, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0A0H3DJX7" ], "nt": "TTGAAAGTTTTATTTACTTTCCAAACCTTACAAAGAAAGGAGGATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCGAAGAAAAGCTTAAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTACTGATAACGAAGTAAAAAGCGAGGAAAAGCCTTTAGGCACTTGA", "aa": "MKVLFTFQTLQRKEDLNMKKVKALRNLFLEKAEEKLKYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVTDNEVKSEEKPLGT", "aa_hexdigest": "bf2e33f63321979240f6528f7b20ff2f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0H3DJX7", "query_cov": 1.0, "subject_cov": 0.9647058823529412, "identity": 1.0, "score": 156.0, "evalue": 2.89e-48, "valid": true, "uniref50_id": "UniRef50_A0A0H3DJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9589.156999999996, "isoelectric_point": 8.663857841491701 }, "id": "CNOCLNGIAL_250", "locus": "GCA000143945_00211" }, { "type": "cds", "sequence": "contig_1", "start": 117197, "stop": 117499, "strand": "+", "frame": 2, "gene": null, "product": "Adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75267", "UniRef:UniRef90_A0A0H3DN54" ], "nt": "TTGCTGTTGGTATTTTTATTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGACTGTTTTGGACCAGGTGTTGGATTATGTTCCCTGGATTGGGAATGGGTACAGGTATGGGAATGACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAACGTCCAAGCGACGCTCGGGGGCAGTCAGACGACGACTACCAAGGGTTCGCCTTGA", "aa": "MLLVFLFSPTLSRFSTGFNLAGTVLDQVLDYVPWIGNGYRYGNDHRGSNSSTSGVTTQGQSQNASSNEPAPTFSNVGVGLKANVQATLGGSQTTTTKGSP", "aa_hexdigest": "47290fd7a38e5beb455e8da216e2716a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 117197, "stop": 117802, "observations": { "mutation": [ 117497, 117530, 117545, 117782 ], "stop": [ 117497, 117530, 117545, 117782 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A0H3DN54", "query_cov": 1.0, "subject_cov": 0.4291845493562232, "identity": 1.0, "score": 192.0, "evalue": 3.05e-60, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MLLVFLFSPTLSRFSTGFNLAGTVLDQVLDYVPWIGNGYRYGNDHRGSNSSTSGVTTQGQSQNASSNEPAPTFSNVGVGLKANVQATLGGSQTTTTKGSPWRTLDPANLQLWTGAGWRNDKASNKQSDENHTTFKSATGMGQQGQSGTSAGNPDALKQDKITKENSGNLKTEDGTATNSTTAFASNYTNLPPTADWPNALSFTNKNNAQRAQLFLRGLLGSIPVLVNKSGENN" }, "paralog": false, "identity": 0.9751243781094527, "score": 389.0, "evalue": 2.8e-140, "description": "Internal stop codon at 117497, 117530, 117545, 117782. Nonsense mutation around 117497, 117530, 117545, 117782.", "psc": { "uniref90_id": "UniRef90_A0A0H3DN54", "product": "Adhesin P1", "uniref50_id": "UniRef50_P75267", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A0H3DN54", "UniRef:UniRef50_P75267" ] }, "pscc": { "uniref50_id": "UniRef50_P75267", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75267" ], "product": "Putative adhesin P1-like protein MPN_131" } }, "id": "CNOCLNGIAL_251", "locus": "GCA000143945_00212" }, { "type": "cds", "sequence": "contig_1", "start": 117614, "stop": 117784, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCCGATGCGTTAAAGCAGGATAAAATCACCAAGGAAAACTCGGGCAATCTCAAAACGGAGGATGGTACTGCTACTAATTCGACGACGGCATTCGCGTCGAATTACACCAACCTCCCCCCCACCGCTGATTGA", "aa": "MGQQGQSGTSAGNPDALKQDKITKENSGNLKTEDGTATNSTTAFASNYTNLPPTAD", "aa_hexdigest": "e2ae73612d4049fbec43e0081a590a36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5746.030300000004, "isoelectric_point": 4.654795265197754 }, "id": "CNOCLNGIAL_252", "locus": "GCA000143945_00213" }, { "type": "rRNA", "sequence": "contig_1", "start": 118294, "stop": 119809, "strand": "+", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9889106327462491, "score": 1446.2, "evalue": 2.1e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGAAAGTAGTAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAGACTAGCTAATACCGCATAAGAACTTTGGTTCGCATGAATCAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTGGGGTAACGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTAAGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTTTAGCAGGTAATGGCTAGAGTTTGACTGTACCATTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATTAATCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGGGCGATCCCCTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTCTAGCGAGACTGCTAATGCAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTCGCCAGCTTGTAAAAGTGAGCAAATCTGTAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGTCGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCATTAGGAAGCGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTT", "id": "CNOCLNGIAL_38", "locus": "GCA000143945_00214" }, { "type": "rRNA", "sequence": "contig_1", "start": 120037, "stop": 122942, "strand": "+", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9935042735042735, "score": 2678.6, "evalue": 8.6e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "CAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAATAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGTAGGTGGTAAGAACCTCAGATCCGGAGATTTCCGAATGGAGCAATCCGGTAGTTGGAAACAGCTATCATTAATTGATGAATAAATAGTCATATTAAAGCAATACGTGGTGAAGTGAAACATCTCAGTAGCCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACTTATCATTAGATAGGGGTTGTAGGGCTTGCAATGTGGACTTGAAAACGATAGAAGAAGCTGTTGGAAAGCAGCGCGCAAAAGGGTGATAGCCCCGTATTTGAAATTGTTTTCATACCTAGCGAGATCCCTGAGTAGCTCGGAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAAAGAGCGTTTGTTTGTTATTATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATCAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTGAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTCGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATTTATATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAAAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTTTCCACCCCCATAATTGGATTTGGGGATAAATCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACATTGAGTGATGATGTCGAGTGAATGAGTGATCAAGTAGCGAAGGTGGTATTAATCATGCTTTCAAGAAAAGCTTCTAGGGTTAATCTAGCTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATGTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAGGACATTATCATGTAGAGAATAGGTAGGAGCAATCGATGCAAGTTCGCTAGGACTTGTTGATGCGAAAGGTGGAATACTACCCTTGGTTGTGTGCTGTTCTAATTGGTAACTGTTATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAATGGAGGCGTACAAAGGTACCTTCAGTACGGTTGGAAATCGTATGTAGAGTGTAATGGTGTAAGGGTGCTTGACTGTGAGACATACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTCCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCGTAGGAAGATTGAAGAGTGTTGCTTCTAGTACGAGAGGACCGAAGCGAGGACACCTCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCGCAAGAAAGTAAGAGCCGTCAAAGACGATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGA", "id": "CNOCLNGIAL_40", "locus": "GCA000143945_00215" }, { "type": "rRNA", "sequence": "contig_1", "start": 122980, "stop": 123087, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.907563025210084, "score": 71.9, "evalue": 1e-11, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TTTGGTGCCCATGTCGCTGTGGAAACACCTGGTTCCATTTCGAACCCAGCAGTTAAGCACAGTGGAGCCGAATGTAGCTGTTTCAGTGAGAATAGGAAAGCACCAAGC", "id": "CNOCLNGIAL_39", "locus": "GCA000143945_00216" }, { "type": "cds", "sequence": "contig_1", "start": 123238, "stop": 123537, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTACTAGTCAAAACCAAAGCCGATTTTTTCATGAACCAACAATTAAATACCACCAGGAAAAGTACAGCTGCTAGGGGCAGAATGGGACTTGTTGGTGGTATTTTACTCGTCATTGGTACCTGCATAGGGGCAGGTATTTTCTTTAAGAGTGAACGGGTGTTGCAAAACATGGGCGGAAACACCACTTTAGCCTTACTGGTGTGGTTAATGGCAGGGATTACCGTGATCTTAATGGGGTTGGCGCTGGTTGAAATTACTGCTAAAGCAGCCTTTGATGACTTGGCATTATTGTCATGA", "aa": "MVLVKTKADFFMNQQLNTTRKSTAARGRMGLVGGILLVIGTCIGAGIFFKSERVLQNMGGNTTLALLVWLMAGITVILMGLALVEITAKAAFDDLALLS", "aa_hexdigest": "79253b3f25f17ac7ef6f4756829d1455", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10516.56659999999, "isoelectric_point": 9.857876777648926 }, "id": "CNOCLNGIAL_253", "locus": "GCA000143945_00217" }, { "type": "cds", "sequence": "contig_1", "start": 123853, "stop": 124161, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGCAAGTGCTGAACAAATGGAACGCCAACCAGTGGTTCCAGTCAAAGATTTTACTGCCATTTTACCCTTCTTTGGTTGGTTTAGTGCTATGGGCGCGATTTTCTTTGCTTTTGATGGTTTTTACGTGTCAGCAGCAGCGAAAACGCAGTTGAAAAAACCAAAAAAATTACCGCAAGTAATGTTGTTGGGCTTAGGGATTGTGGTACTGATTTACAGCTTAATTGCACTAAGTGTGAGTTTAACCACACCTAACGGTGCCTTTTCGGGACTTGGTGATTGGTTAAAGCACAAAAAGCTTGGTTGA", "aa": "MGASAEQMERQPVVPVKDFTAILPFFGWFSAMGAIFFAFDGFYVSAAAKTQLKKPKKLPQVMLLGLGIVVLIYSLIALSVSLTTPNGAFSGLGDWLKHKKLG", "aa_hexdigest": "409e08a559f3df398c3684a0b65f9fa4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11022.017599999992, "isoelectric_point": 9.756339073181149 }, "id": "CNOCLNGIAL_254", "locus": "GCA000143945_00218" }, { "type": "cds", "sequence": "contig_1", "start": 124321, "stop": 124767, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTTTAATCCATGCAGGGATTTTGATGGTGTTAACTACCGTAGCACTTTCTAGTTTAGGTGGTTTGTTGTACTTACCTAAAGTTAATGCTTCCTATGACGGCAAGGGCTTTAAATCAATGGGGTGCTTACTGGAGTTTGCTGACTTAATTGCCACTTGGACTAGTGTCGGGATCTTTTGGTTCTTGGGCTTAGTTTTACTGGGTGGTTTATTGCAAATCAAGAAACCGAAGCGGTGGTATTTTAGAACAACGGGTTGGCTAGCTGTAGTAGTGATTGGTCTCACTACTTTAGTGGTCATGGTGCAACCGTTTGTAGATTTAGGAATAGCAGTGTTTAACCGTAGTTATGAACGGATTGTGGCTAACACTATTTTGATTGCTATTCTTGTGATAATTGTTTTAGTAATGTTCTTTCCAACCGAACCAATTAAGCTAAGACTTTGA", "aa": "MGLIHAGILMVLTTVALSSLGGLLYLPKVNASYDGKGFKSMGCLLEFADLIATWTSVGIFWFLGLVLLGGLLQIKKPKRWYFRTTGWLAVVVIGLTTLVVMVQPFVDLGIAVFNRSYERIVANTILIAILVIIVLVMFFPTEPIKLRL", "aa_hexdigest": "e330b724742793b6c8bf951155f9565f", "start_type": "GTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16314.708199999994, "isoelectric_point": 9.77651767730713 }, "id": "CNOCLNGIAL_255", "locus": "GCA000143945_00219" }, { "type": "cds", "sequence": "contig_1", "start": 125006, "stop": 125641, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTTAAGTATGGTGCGCTAATTTTTAGCGGCTTTTTAGGACTTTCAATAGTTCTAGCTAGTTGTGGGGCGAGGGGTAAATTTGATCAAGTTGATGATGGTAAGATTAAGCTAGCTTCATCTTTAACTTCTAGAAGTGCGTCAGCGGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGTGTAAATGATTACCCCATTGAGATTACCCAGATTGCTGGTGGTTATGATGGCGGGCGAACTGATCTCCAAACCCGGGTTAGTGTAAAGGATAAAACCAGCTTTTACAACATGATTCTAAACTACCCTGATTTAGTGTCGGTGTTAGCCCGTAACGGGATGGAATTACCGTTTGATGGAGTCAATGTTGACAAGTTATCTCCCAACTTCTTAAAGTTCAATGAACGCATTAGTGGCGTAGCGAAAAAAGCTAACTATGCCATTCCCATTTCGATGTCAACCGATATCTTAATCCTGAATGCACCGGTGTTGCACTATATTTTGAACAGTGTTAAGAAGAATGATGGCAACACTAAAGTTCAAGTTAAAGCTCAGTCAAAAGATTCACAAACAAAAGTTAAAGGTACTATGGAAATTGGCACTGATGAAAGTACTAAAAACTTATGA", "aa": "MQFKYGALIFSGFLGLSIVLASCGARGKFDQVDDGKIKLASSLTSRSASAALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVSVKDKTSFYNMILNYPDLVSVLARNGMELPFDGVNVDKLSPNFLKFNERISGVAKKANYAIPISMSTDILILNAPVLHYILNSVKKNDGNTKVQVKAQSKDSQTKVKGTMEIGTDESTKNL", "aa_hexdigest": "8dd927650f18be9a3c50385b1f965cb6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22995.160200000017, "isoelectric_point": 9.421361351013182 }, "id": "CNOCLNGIAL_256", "locus": "GCA000143945_00220" }, { "type": "cds", "sequence": "contig_1", "start": 125882, "stop": 126631, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75595", "UniRef:UniRef90_P75595" ], "nt": "TTGATCGATTTTTCAACGCGCGTAGCAAAATCATTTAAAAACAAAGTTAGTGATATTTCCACTAAAAAGGGCACTGACATTCAGGGCGTTTTAGGTCTAGACAGTACACCAAATGCTTTGTTTACCTCAGTTTTTGCTGCAGGTGATAGTAACTTTGATAACTTCTTTTATAAGGTTAAAGATGGTCGCGCTGACTTTAGTAACTTTAACGAAAACGGTACCTCTTACAAAAATCTGGAAAAGGTTTTTAATGACTATAAAAAACTCACAGACAGCAACGGTTTGTTTGTCAATAAAGGGGGTTCTTACACCTCTAACTTCCAGAAGTTTCACCAGTTAGCCTACAGCATTTCCTCCTCTTCTGGTTATGCTTATGCGTTTGCTGGTGAAAATTCAAAACGTTTAAAGTTCAACGACGATACTTTCATTGAGTACCCTAGTTTTACCCAGGAAATTCATGCGCCTGGTCAAAGTAGTCAAAAAGAAGGTGGACAGCAACAAAGCAACAGTAAAGATAACGGCAATCTTTTAGGCACTTTTACTATTGAAGCAGCGAAAAGTAAAACAAAAACAGAAGTTAAAAAAACTGAAGACACACAAAATCAAGGCAAGAAGGCTGAAGGCACACCTAACCAAGGTAAAAAAGCCGAAGGGACCGAAAACCAAGGTAAGACTATTTTCCTTTATAAAACATCAATTCCTAATGATAAGCAAGATGGTGTTGACGCTGTATTAATTAAAGACAAGTAG", "aa": "MIDFSTRVAKSFKNKVSDISTKKGTDIQGVLGLDSTPNALFTSVFAAGDSNFDNFFYKVKDGRADFSNFNENGTSYKNLEKVFNDYKKLTDSNGLFVNKGGSYTSNFQKFHQLAYSISSSSGYAYAFAGENSKRLKFNDDTFIEYPSFTQEIHAPGQSSQKEGGQQQSNSKDNGNLLGTFTIEAAKSKTKTEVKKTEDTQNQGKKAEGTPNQGKKAEGTENQGKTIFLYKTSIPNDKQDGVDAVLIKDK", "aa_hexdigest": "895fc2063a6d9c9b6b57d5f5d34dc806", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 125582, "stop": 126631, "observations": { "mutation": [ 125639, 125873 ], "stop": [ 125639, 125873 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75595", "query_cov": 1.0, "subject_cov": 0.4602587800369686, "identity": 0.996, "score": 425.0, "evalue": 3.96e-145, "gene_start": 1, "gene_end": 249, "reference_start": 293, "reference_end": 541, "reference_sequence": "MQFKYGALIFSGFLGLSIVLASCGARGKFDQVDDGKIKLASSLTSRSASAALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVSVKDKTSFYNMILNYPDLVSVLARNGMELPFDGVNVDKLSPNFLKFNERISGVAKKANYAIPISMSTDILILNAPVLHYILNSAKKNDGNTKVQVKAQSKDSQTKVKGTMEIGTDESTKNLWSDIQKKAGENGKATTEGTKKAAAKSTHLTLLTKSEQSTQGNNGASESDKKIEETWGTYSEVDGGLKNYTFKADVFDTWHGLIDFSTRVAKSFKNKVSDISTKKGTDIQGVLGLDSTPNALFTSVFAAGDSNFDNFFYKVKDGRADFSNFNENGTSYKNLEKVFNDYKKLTDSNGLFVNKGGSYTSNFQKFHQLAYSISSSSGYAYAFAGENSKRLKFNDDTFIEYPSFTQEIHAPGQSSQKEGGQQQSNSKDNGNLLGTFTIEAAKSKTKTEVKKTEDTQNQGKKAEGTPNQGKKAEGTENQGKTIFLYKTSIPNDKQDGVDAVLIKDK" }, "paralog": false, "identity": 0.994269340974212, "score": 632.0, "evalue": 8.24e-229, "description": "Internal stop codon at 125639, 125873. Nonsense mutation around 125639, 125873.", "psc": { "uniref90_id": "UniRef90_P75595", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75595", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75595", "UniRef:UniRef50_P75595", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75595" ], "product": "Uncharacterized lipoprotein MPN_097" } }, "id": "CNOCLNGIAL_257", "locus": "GCA000143945_00221" }, { "type": "cds", "sequence": "contig_1", "start": 126929, "stop": 127033, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGACTCAAGGTCCTAATCTAATTGGTATCCATACTAATACCAAGGAAAATGAAGAAACGCAAAAGTTTGTTAACTGGTTCTTAAATACCAGTCTAACTTGA", "aa": "MMTQGPNLIGIHTNTKENEETQKFVNWFLNTSLT", "aa_hexdigest": "2047a8bc4f0b3da0607b140375041861", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3938.3996000000006, "isoelectric_point": 5.478164863586425 }, "id": "CNOCLNGIAL_258", "locus": "GCA000143945_00222" }, { "type": "cds", "sequence": "contig_1", "start": 127223, "stop": 127372, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTTACAGTGATCCAAGCGATTTCAGGGGTGGTAAATTCCGCGAATCAATTGGTTCTGCCTTTAACGCCACGGTCAATTCACACGTTGACTTTAATACTTTCGTTGCTAACTTCCGTGCTAACTTAGGTTCTGGTTACGATAAATAG", "aa": "MGYSDPSDFRGGKFRESIGSAFNATVNSHVDFNTFVANFRANLGSGYDK", "aa_hexdigest": "f1815f7d0d0feaaebcea9c9fb1c32008", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5352.7325, "isoelectric_point": 6.516329002380371 }, "id": "CNOCLNGIAL_259", "locus": "GCA000143945_00223" }, { "type": "cds", "sequence": "contig_1", "start": 127344, "stop": 127784, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0010051FB8", "UniRef:UniRef100_A0AB38W7Y3", "UniRef:UniRef50_A0AB38W7Y3", "UniRef:UniRef90_A0AB38W7Y3" ], "nt": "GTGGTTTTCGTTGCCTTAGGTGTTATTCCCAGTAGCCTTATCATTCCTTCACCCCGCCCCCGTTCAGAGCTGGAGGTTGGCTTGGTCGAGGGTGGGTCATTTGGGAATATCTTTCCCCGTAGTCGTCGTCTGACTGCCCCCGAGGGTGGCTTGGACATTCGCTTTGAGACCGACGCCGACATTCGAAAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTTAGGCGCGGTTATATCATCCACACCCCGGTGATCATTCCCATACCTGTGCCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCGAGGAGTGCCCCCGCGAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGGGATTAACTATTTATCGTAACCAGAACCTAAGTTAG", "aa": "MVFVALGVIPSSLIIPSPRPRSELEVGLVEGGSFGNIFPRSRRLTAPEGGLDIRFETDADIRKRREKGTRTTCVRTSSAGRPRAGFRRGYIIHTPVIIPIPVPIPNPGNIIQHLVEECPREVEPGGETGKRGGEVGLTIYRNQNLS", "aa_hexdigest": "ce37e5c0bd7b24764ba1e49899f0ee2a", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0010051FB8", "uniref100_id": "UniRef100_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0010051FB8", "UniRef:UniRef100_A0AB38W7Y3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7Y3", "uniref90_id": "UniRef90_A0AB38W7Y3" }, "psc": { "uniref90_id": "UniRef90_A0AB38W7Y3", "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15948.147299999986, "isoelectric_point": 10.137669563293457 }, "id": "CNOCLNGIAL_260", "locus": "GCA000143945_00224" }, { "type": "cds", "sequence": "contig_1", "start": 128077, "stop": 128358, "strand": "+", "frame": 1, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6", "UniRef:UniRef90_A0AB33HTF6" ], "nt": "GTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCTCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCTTTGGTAGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDSNKFQATDQKWSYTDLQSDQTKLNLSAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGFKIPEQNNDSKATLITPGLA", "aa_hexdigest": "e42bb8ca944d16dfc92afce773255570", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 128005, "stop": 128654, "observations": { "deletion": [ 128434 ], "mutation": [ 128356 ], "stop": [ 128356, 128500 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HTF6", "query_cov": 1.010752688172043, "subject_cov": 0.42727272727272725, "identity": 0.862, "score": 155.0, "evalue": 7.97e-46, "gene_start": 1, "gene_end": 93, "reference_start": 25, "reference_end": 118, "reference_sequence": "MSFTNKNNAQRVQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTLTSSTANQNSTTVPSIGFKIPEQNNDSKAVLITPGLAWTPQDVGNLVVSGTSLSFQLGGWLVTVHGLYQTPRGLFGPPVKWPGCQWQRPKGVNLGQAALSGISWQLGQPVGTGRECVGFEGGVGGSSSQRGVRVASSYK" }, "paralog": false, "identity": 0.8454545454545455, "score": 358.0, "evalue": 2.01e-128, "description": "Internal stop codon at 128356, 128500. Frameshift due to deletion around 128434. Nonsense mutation around 128356.", "psc": { "uniref90_id": "UniRef90_A0AB33HTF6", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HTF6", "UniRef:UniRef50_A0AB33HTF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6" ], "product": "Adhesin P1 homolog" } }, "id": "CNOCLNGIAL_261", "locus": "GCA000143945_00225" }, { "type": "cds", "sequence": "contig_1", "start": 128581, "stop": 129099, "strand": "+", "frame": 1, "gene": null, "product": "Putative adhesin P1-like protein MPN_099", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593", "UniRef:UniRef90_P75593" ], "nt": "GTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTGCCACCACCGCAACAGGGGCTACCTTACCGGAACACCCGAATGCTTTGGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCTCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTCGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTGTTTGGAGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGGCAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACCATTAATAGGAATTTTTCTTATTAA", "aa": "MESVWDLKGVWADQAQLAAQAATSSATTATGATLPEHPNALAYQISYTDKDSYKASTQGSGQTNSQNNSLYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGAMSGNLGSVLSGGGAGGAGSTNSVDLSPVERVSGSLTINRNFSY", "aa_hexdigest": "452dafbf25df93d2f19a4fa40272aaf2", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 128281, "stop": 129099, "observations": { "mutation": [ 128356, 128539 ], "stop": [ 128356, 128539 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75593", "query_cov": 1.005813953488372, "subject_cov": 0.4357682619647355, "identity": 0.988, "score": 318.0, "evalue": 2.45e-106, "gene_start": 1, "gene_end": 172, "reference_start": 225, "reference_end": 397, "reference_sequence": "TTQDGNATGQQEATNYTNLPPNLTPTADWPNALSFTNKNNAQRAQLFLRGLLGSIPVLVNRSGSDSNKFQATDQKWSYTDLQSDQTKLNLSAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGFKIPEQNNDSKATLITPGLAWTPQDVGNLVVSGTTVSFQLGGWLVTFTDFVKPRAGYLGLQLSGLDASDSDQRELIWAPRPWAAFRGSWVNRLGRVESVWDLKGVWADQAQLAAQAATSSATTTATGATLPEHPNALAYQISYTDKDSYKASTQGSGQTNSQNNSLYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGAMSGNLGSVLSGGGAGGAGSTNSVDLSPVERVSGSLTINRNFSY" }, "paralog": false, "identity": 0.989010989010989, "score": 532.0, "evalue": 5.78e-193, "description": "Internal stop codon at 128356, 128539. Nonsense mutation around 128356, 128539.", "psc": { "uniref90_id": "UniRef90_P75593", "product": "Putative adhesin P1-like protein MPN_099", "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75593", "UniRef:UniRef50_P75593" ] }, "pscc": { "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593" ], "product": "Putative adhesin P1-like protein MPN_099" } }, "id": "CNOCLNGIAL_262", "locus": "GCA000143945_00226" }, { "type": "cds", "sequence": "contig_1", "start": 129187, "stop": 129438, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_015344874.1", "SO:0001217", "UniParc:UPI0002B8420C", "UniRef:UniRef100_A0AB38WA43", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0A0H3DNZ5" ], "nt": "ATGAAATTAGTTAAATTAACGAGGCTTCACCGCTTTTTAAAACAAGTTCAATTGCAACTTGTTCCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTATTGGCGCTTCTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCAGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAA", "aa": "MKLVKLTRLHRFLKQVQLQLVPLFSQNFLQVLLALLNKPVNLFSENTTMKTNKKYAFAALLMQAQAKLPNSFKFFLSKKGGMA", "aa_hexdigest": "18d85a11dacc9036768befe2855820f5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B8420C", "ncbi_nrp_id": "WP_015344874.1", "uniref100_id": "UniRef100_A0AB38WA43", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B8420C", "RefSeq:WP_015344874.1", "UniRef:UniRef100_A0AB38WA43" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38WA43", "uniref90_id": "UniRef90_A0A0H3DNZ5" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DNZ5", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9528.455399999993, "isoelectric_point": 10.966668510437014 }, "id": "CNOCLNGIAL_263", "locus": "GCA000143945_00227" }, { "type": "cds", "sequence": "contig_1", "start": 129606, "stop": 129989, "strand": "+", "frame": 3, "gene": null, "product": "UPF0134 protein MPN_100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254", "UniRef:UniRef90_P75592" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAATTTAACGAATATAAAGTTTCAAACGACCAACGGCTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAGGTTGAGCAAATTAAAGCTCAAGGAAAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCACTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAG", "aa": "MNTEYVTRKEFNEYKVSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQVEQKAQVEQIKAQGKQIKAQGKTLKSILQALGGINKRLDKIDPPK", "aa_hexdigest": "ebca61d28ac22e2cebbba92a3f60f342", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 129438, "stop": 129989, "observations": { "mutation": [ 129519 ], "stop": [ 129519 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75592", "query_cov": 1.0, "subject_cov": 0.6939890710382514, "identity": 0.89, "score": 95.5, "evalue": 2.68e-22, "gene_start": 1, "gene_end": 127, "reference_start": 57, "reference_end": 183, "reference_sequence": "MKEKIPFYNEKEFHDMVKKTKKGTFSGWYIIDKDNKSVEFSGSFNRQFKLNKPVIPVNTEYVTRKEFNEYKVSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQVEQKAQGEQIKAQGKQIKAQGKTLKSILQALGGINKRLDKIDPPK" }, "paralog": false, "identity": 0.9234972677595629, "score": 211.0, "evalue": 2.32e-70, "description": "Internal stop codon at 129519. Nonsense mutation around 129519.", "psc": { "uniref90_id": "UniRef90_P75592", "product": "UPF0134 protein MPN_100", "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75592", "UniRef:UniRef50_P75254" ] }, "pscc": { "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254" ], "product": "UPF0134 protein MPN_524" } }, "id": "CNOCLNGIAL_264", "locus": "GCA000143945_00228" }, { "type": "cds", "sequence": "contig_1", "start": 130446, "stop": 130703, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGACAACAGCGCCAAGGGCATCACTGCCGGTAGTGAAAGTCAACAAACCACGTATGATCCCACTCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTAGTAGGTTAAACCCACAGACACCAACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNSAKGITAGSESQQTTYDPTRTEAALTASTTFALRRYDLAGRALYDLDFSRLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "b905c138d040e7b9e91ea82d31269fa1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 4.914575641049703e-09, "score": 31.38545799255371, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9190.931099999994, "isoelectric_point": 6.072189140319824 }, "id": "CNOCLNGIAL_265", "locus": "GCA000143945_00229" }, { "type": "cds", "sequence": "contig_1", "start": 130734, "stop": 131252, "strand": "+", "frame": 3, "gene": null, "product": "Adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571", "UniRef:UniRef90_A0AAX0SQU5" ], "nt": "GTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGAACAAGGCACCGAACAGGTGGAACTTGAATCTAATGATCTCGCCAACGTTCCCATTAAGCGGGGCGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGGSSGDTKVKALQVAVKKKSGSQGNSGEQGTEQVELESNDLANVPIKRGEESGQSVQLKAADFGTTPSSSGSGGNSNPGSPTP", "aa_hexdigest": "14416f07bfcd6de52dccf2fbaea94ead", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "both", "pseudogene": { "start": 130446, "stop": 131555, "observations": { "mutation": [ 130701, 131250, 131298, 131412, 131427, 131451 ], "stop": [ 130701, 131250, 131298, 131412, 131427, 131451 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0SQU5", "query_cov": 1.0, "subject_cov": 0.4047058823529412, "identity": 0.9420000000000001, "score": 271.0, "evalue": 1.16e-87, "gene_start": 1, "gene_end": 172, "reference_start": 97, "reference_end": 268, "reference_sequence": "MRDNSAKGITAGSESQQTTYDPTRTESALTASTTFALRRYDLAGRALYDLDFSRLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKNKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGGSSGDTKVKALQVAVKKKSGSQGNSGEQGTEQVELESNDLANVPIKRGEESGQSVQLKAADFGTTPSSSGSGGNSNPGSPTPWRPWLATEQIHKDLPKWSASILILYDAPYARNRTVIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDQGQAVADNTQTGFDTDDTDNKKQGFQKEADSDKPAPIALPFEAYFANIGNLTWFGQALLVFGICLS" }, "paralog": false, "identity": 0.959349593495935, "score": 680.0, "evalue": 5.68e-251, "description": "Internal stop codon at 130701, 131250, 131298, 131412, 131427, 131451. Nonsense mutation around 130701, 131250, 131298, 131412, 131427, 131451.", "psc": { "uniref90_id": "UniRef90_A0AAX0SQU5", "product": "Adhesin P1", "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0SQU5", "UniRef:UniRef50_P75571" ] }, "pscc": { "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571" ], "product": "Uncharacterized protein MPN_205" } }, "id": "CNOCLNGIAL_266", "locus": "GCA000143945_00230" }, { "type": "cds", "sequence": "contig_1", "start": 131214, "stop": 131690, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCGAACCAGGTGAGGTTGCCAATGTTGGCGAAGTACGCTTCAAACGGGAGGGCGATCGGGGCCGGCTTGTCGGAGTCAGCTTCCTTTTGAAAGCCTTGTTTTTTATTATCGGTGTCATCTGTATCAAACCCGGTTTGGGTATTATCCGCGACCGCTTGGCCCTGGTCAAACCACTCGGGGTGGCGCCGCGGGTTGAAGAACCCGGTGGTTTGGAGCAAAACATCTCTGGCCTTTCAGTCCCACAACCCGTGGTGGTTTCACTTGGGCGTTCTTCAACTGGGCGGATAGTTCGCGGTCATCACCTTGGGATCCAAGTGATCAACGCGATCAATGGCGGTACGATTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGATCATTTGGGGAGGTCCTTGTGAATTTGCTCGGTCGCAAGCCACGGCCTTCAGGGGGTGGGGGAACCGGGGTTGGAGTTGCCGCCTGA", "aa": "MPEPGEVANVGEVRFKREGDRGRLVGVSFLLKALFFIIGVICIKPGLGIIRDRLALVKPLGVAPRVEEPGGLEQNISGLSVPQPVVVSLGRSSTGRIVRGHHLGIQVINAINGGTIARIRRIVQNQDRGGSFGEVLVNLLGRKPRPSGGGGTGVGVAA", "aa_hexdigest": "df041ee149f544e157686e38763791f5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16507.10239999998, "isoelectric_point": 11.397381782531738 }, "id": "CNOCLNGIAL_267", "locus": "GCA000143945_00231" }, { "type": "cds", "sequence": "contig_1", "start": 131866, "stop": 132303, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAGGCTTAATGATGGGACGAGTTCGGATTCCAATACCACCACTGATGGGGCGCTTACGTTTGACACGAATGAAAGTCAAAAAGCTTTAGGCTCCACGGACACGCAGGGTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGA", "aa": "MGQNKLVALVRSALVRVRLNDGTSSDSNTTTDGALTFDTNESQKALGSTDTQGSGSNSQDFASYILIFKAAPRATWVFERKIKLALPYVKNESGAGDSTTTNSGSLYKTLQDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESG", "aa_hexdigest": "fca8adc49ca3ff2021bc087765450749", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15382.876899999977, "isoelectric_point": 6.564755821228027 }, "id": "CNOCLNGIAL_268", "locus": "GCA000143945_00232" }, { "type": "cds", "sequence": "contig_1", "start": 132415, "stop": 133026, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028006D2D" ], "nt": "GTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTTCTAGTACCAGTTTGGGCAATGTTGGGGACACCACTGCCCTAACTCCACTTTTAAGAGGTGCTACTACTACTACTACCGTCCAGCTAAAACAAACAGATTCTAACAGTCAAGATCAACAAAAATTCCAAAAATATTTAAACACCGCCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCAGAACTTAGCCAAGGCGGTTGGCAAAGCCAAACAGCGCGGCACAGTTCCACCCGGGGACTACAGTCAGCCGGGGCCAATCGTGGCGCTAGTTTAGCGGCACGCACCACCACAACTGTGGGGGTGGGTAGAAGAGATTCCACTTCCACCACCTTGGAACTACCAAACGTCATCACCCAGCTTTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGA", "aa": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGSTGSQMASSTSLGNVGDTTALTPLLRGATTTTTVQLKQTDSNSQDQQKFQKYLNTAQALHQMGVIVPELSQGGWQSQTARHSSTRGLQSAGANRGASLAARTTTTVGVGRRDSTSTTLELPNVITQLYHTSTSQLAYLNGQIVVMGSNAVPSLWYWVVDERTTSGRAT", "aa_hexdigest": "b224550d7c52e30fbc1d463a16f63d81", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028006D2D", "query_cov": 0.8275862068965517, "subject_cov": 0.9767441860465116, "identity": 0.5710000000000001, "score": 142.0, "evalue": 8.29e-40, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0028006D2D" }, "pscc": { "uniref50_id": "UniRef50_UPI0028006D2D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028006D2D" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_269", "locus": "GCA000143945_00233" }, { "type": "cds", "sequence": "contig_1", "start": 133042, "stop": 133575, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W870" ], "nt": "TTGAACTTCGGCACTGAAGTTCAAAAAAACTTCGTCGAAAACCAGTTGGGGTTTAAATCCGAAGACAACTCCAACACCTCCTTGACGAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACTTAATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAACAACCTGGTGACGAATACTGCGGGGTTAAACGGTCCGATTAACGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATAACGAAGAAGTGCAAACGACCTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCGTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATAATGGCGATAAAAAAGCCATCCACCTAAGGTGA", "aa": "MNFGTEVQKNFVENQLGFKSEDNSNTSLTNFKSQGLTQPAYLISGLDVVADHLVFAAFKAGAVGYDMTTDSNASTYNQALTWSTTAGLDSDGGYNNLVTNTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSKNNEEVQTTYPVKSDQKATAKIASLINASPLNSYGDNGDKKAIHLR", "aa_hexdigest": "25cda666e9126270a252a3b66701221c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W870", "query_cov": 0.8813559322033898, "subject_cov": 0.8571428571428571, "identity": 0.7120000000000001, "score": 189.0, "evalue": 3.02e-58, "valid": false, "product": "MgpC protein", "uniref50_id": "UniRef50_A0AB38W870" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W870", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W870" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_270", "locus": "GCA000143945_00234" }, { "type": "cds", "sequence": "contig_1", "start": 133652, "stop": 133870, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00059C39D0", "UniRef:UniRef100_A0AB38W8B4", "UniRef:UniRef50_A0AB38W8B4", "UniRef:UniRef90_A0AB38W8B4" ], "nt": "ATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTACCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAGTAGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTACAACTCCATCTAACTATTAGTAGGGTTTTCACTTTTGAAAATTTTTTTGGATTAATTTTGGCACCCCGCCTTTTTTAA", "aa": "MNLLNLFKSAGAGSFFQHKSLYLLPLNKLRQIFKVAKEFEYFCNLGLQLHLTISRVFTFENFFGLILAPRLF", "aa_hexdigest": "6c7d16b8c20ece8415b2c8db7655b76c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00059C39D0", "uniref100_id": "UniRef100_A0AB38W8B4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00059C39D0", "UniRef:UniRef100_A0AB38W8B4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8B4", "uniref90_id": "UniRef90_A0AB38W8B4" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8B4", "uniref50_id": "UniRef50_A0AB38W8B4" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8B4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8415.915199999992, "isoelectric_point": 9.897847175598145 }, "id": "CNOCLNGIAL_271", "locus": "GCA000143945_00235" }, { "type": "cds", "sequence": "contig_1", "start": 134108, "stop": 134683, "strand": "+", "frame": 2, "gene": null, "product": "DUF16 family-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75565", "UniRef:UniRef90_A0AB38W7Z0" ], "nt": "ATGATGAGTAAAAATAAATTCTTTACCATTACTAAAGAACAGTACAGGAAGTTTACACGCAATAAAATTGAACTTCTTTTTAAGGTCCAATGTACCGAAAAGAAAAAGGGTAAACGTAGGTTCAAAATATTTCAGCAAGTCGAGGATAAACCAAAACCACCAGTTAAAATAATTGAAAAGGAATCTAAGGGACCGGAAGAACCAAAGGATCCTGGTATTAAAAATGGACCTAAGGGTCCCGGTGGACCAAAAGGACCTAAAAAGCCACCTGTTCCCGATGATCCTGATACACCAGATGTTCCTAAAGGCCCCAAAGAACCAGGCGGTTCAAAAGGTCCAGGTGGTCCCGATGATCCTAATGGAGGAAAGAAGAAAGTGCCTAAACCAGATAAGTATGTAACCCATCGCGAGCTTGATGAGAAACTTACTGAGTTTCATGACAAGCTTATGGTAGAACTTGATGGTAAGTTTGCTACTAAAGCTGATTTAAAACGGGTTGAAGATAAAGTTGATGTTTTGTTTGAATTGCAAAAAACGCAAGATGAACAAATCAAGGTTCAAGGAAAACAAATCTAA", "aa": "MMSKNKFFTITKEQYRKFTRNKIELLFKVQCTEKKKGKRRFKIFQQVEDKPKPPVKIIEKESKGPEEPKDPGIKNGPKGPGGPKGPKKPPVPDDPDTPDVPKGPKEPGGSKGPGGPDDPNGGKKKVPKPDKYVTHRELDEKLTEFHDKLMVELDGKFATKADLKRVEDKVDVLFELQKTQDEQIKVQGKQI", "aa_hexdigest": "50833bf10d8c9adc3ac0f437fc726cad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W7Z0", "query_cov": 0.9947643979057592, "subject_cov": 1.0, "identity": 1.0, "score": 261.0, "evalue": 2.5e-86, "valid": true, "product": "DUF16 family-like protein", "uniref50_id": "UniRef50_P75565" }, "pscc": { "uniref50_id": "UniRef50_P75565", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75565" ], "product": "UPF0134 protein MPN_104" }, "genes": [], "id": "CNOCLNGIAL_272", "locus": "GCA000143945_00236" }, { "type": "cds", "sequence": "contig_1", "start": 135066, "stop": 135245, "strand": "+", "frame": 3, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCY4", "UniRef:UniRef90_A0AAN5BCY4" ], "nt": "ATGAAAGATGAAACAAATATTTGTTCTCAATCTTTAAAAAGACCTTTAAGCGAAGACCAATTGATAGAGTATTTGAGTACTTATGAAATAACTTTATTTGAGGTTTATGCTGAGGATATGCTAGATGGCAATTGCAAAATTTATATACATGCTAAAGGACGTCCAAAAGACCCAGATTGA", "aa": "MKDETNICSQSLKRPLSEDQLIEYLSTYEITLFEVYAEDMLDGNCKIYIHAKGRPKDPD", "aa_hexdigest": "402782f5382e4b9b10aba387c56fea46", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 135066, "stop": 135332, "observations": { "mutation": [ 135243 ], "stop": [ 135243 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN5BCY4", "query_cov": 1.0, "subject_cov": 0.6704545454545454, "identity": 1.0, "score": 122.0, "evalue": 2.25e-35, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MKDETNICSQSLKRPLSEDQLIEYLSTYEITLFEVYAEDMLDGNCKIYIHAKGRPKDPDWKRKTDKKSLEIERYVKKFIKFLKREQKK" }, "paralog": false, "identity": 0.9886363636363636, "score": 177.0, "evalue": 3.45e-59, "description": "Internal stop codon at 135243. Nonsense mutation around 135243.", "psc": { "uniref90_id": "UniRef90_A0AAN5BCY4", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAN5BCY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN5BCY4", "UniRef:UniRef50_A0AAN5BCY4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAN5BCY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCY4" ], "product": "Family K-like protein" } }, "id": "CNOCLNGIAL_273", "locus": "GCA000143945_00237" }, { "type": "cds", "sequence": "contig_1", "start": 135887, "stop": 136729, "strand": "+", "frame": 2, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_P75564", "UniRef:UniRef90_P75564" ], "nt": "TTGGAAAGCACCTTGCAAACACTCCATGATACACAGCTACAAGTCTTTACCCAAGCCCAGTCTAGCAGTGTATTAACTCATGGTGATGTGATGTTGCTAGCAACCTCTTTTGCCCCTGGTTCTAGCAACATTATTTACCAGGTAATTGATGAGTTAGTGAACTATTTTAAGAAGTTCTTGTTTACGGTTAACTATGACAGTGAATTGACTACTATTGCAGACTGTTTTGACCTGCTAAACATTCCCAAAGACCATCCTAGTCGTAACCTTACTGACACCTTTTACTTAGACAAAAACCGCTTACTGAGAACACATTGTACTGCTGCTACTTTACGTGCTGTTAAGGAAACGAAAAAGAGTAATAACCCCGATATTCGGATTGCTTCCTTTGGTGCTGTTTTTCGTAAAGATGATGACGATGCCACCCACTCACACCAGTTCAACCAACTGGACTTCATGTGGATTAAAAAGGATTTTTCTTTAACTAACCTCAAGTGGTTTATGCAGAATATGATTAACCACATCTTTGGCGAAAATACTTCAGCGCGTTTTCGTCTGTCCCACTTCCCTTTTACCGAACCTTCCTTTGAAATTGACATCCGTTGCTGGTTGTGTCAGAATGGTTGTGGTGTATGTAAGAAAACACGTTGGATTGAGGTATTAGGGGCGGGTATCCTCCACCCTCAAGTGATGGCTAACATGGGCTTTAGTGACACGGATAATATAAGGGGTATAGCAGCTGGCATTGGGATTGAGCGGTTGGTAATGTTGAAACACGGCATTAGTGACATCCGTGACCTTTACGACAATAACTTCAAATTTTTAGCCCAGTTTACTGATTAG", "aa": "MESTLQTLHDTQLQVFTQAQSSSVLTHGDVMLLATSFAPGSSNIIYQVIDELVNYFKKFLFTVNYDSELTTIADCFDLLNIPKDHPSRNLTDTFYLDKNRLLRTHCTAATLRAVKETKKSNNPDIRIASFGAVFRKDDDDATHSHQFNQLDFMWIKKDFSLTNLKWFMQNMINHIFGENTSARFRLSHFPFTEPSFEIDIRCWLCQNGCGVCKKTRWIEVLGAGILHPQVMANMGFSDTDNIRGIAAGIGIERLVMLKHGISDIRDLYDNNFKFLAQFTD", "aa_hexdigest": "8632576b4a53936657e85c6b3d35df72", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75564", "query_cov": 1.0, "subject_cov": 0.8211143695014663, "identity": 0.996, "score": 567.0, "evalue": 1.53e-203, "valid": true, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_P75564", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_P75564", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75564" ], "product": "Phenylalanine--tRNA ligase alpha subunit" }, "genes": [ "pheS" ], "id": "CNOCLNGIAL_274", "locus": "GCA000143945_00238" }, { "type": "cds", "sequence": "contig_1", "start": 136732, "stop": 137463, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTAATTTCTAAAAAAACTTTAGCTGTTTTAATTCCCGAAATTACCCACGTTTCCAACAATGAAATTTGTGAAAAGTTACAGCAAATCGGGATTGAAGTGGAAGCAATTCGCGCCTTTAAAAACCCTGATTACTTACAGTTGGGTATTCTCCGCGCTGTTACCCCCCACCCCCATGACAACCACTTGTATGTGTGTCAAGTTCAAATAGACAAAAACAAACAGCTCAATGTGGTGACTGGGGCAGTTAACATAGTTGATCCCAATAATCTCAATAAACACGTGATTGTCGCCAAAAAAGGGGCAGAACTGCTCAATGGTTTAATCATTAAAACGAAGAACATTAAGGGAATTATCTCGGACGGGATGTTGTGTAGTTATGTTGATATTAATCCGTTTAGTAAGCATTTAATTGCTGACGGTGATGACACGCATGCGATTGTGCTAGACAACATTAACCGCGATGAATTTGGTGATTATTTAAGTTTTTTAAACCTCGATGACGTGGTGTTTGAGGTTACTTTACCTACCAACCGTTCTGATTTACAGAGCTTAATCTTCCTCGCTAAGGAACTAGCAGCAGTGTTGAAACGTCCGATCTTCTTGGAACAGAAAACCACCATGACCCTGCGCGAGTTCTACCGCTTTCCGCTTAACTTAAGAAACCGTGCTCAGGCCAATTTCTTTGGTGGACTTTTTTTGCGTGATGTAGCAATTACCAGTTCCCCTTGA", "aa": "MLISKKTLAVLIPEITHVSNNEICEKLQQIGIEVEAIRAFKNPDYLQLGILRAVTPHPHDNHLYVCQVQIDKNKQLNVVTGAVNIVDPNNLNKHVIVAKKGAELLNGLIIKTKNIKGIISDGMLCSYVDINPFSKHLIADGDDTHAIVLDNINRDEFGDYLSFLNLDDVVFEVTLPTNRSDLQSLIFLAKELAAVLKRPIFLEQKTTMTLREFYRFPLNLRNRAQANFFGGLFLRDVAITSSP", "aa_hexdigest": "856a218a20336e72f3cc3f8d7bcfb2ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 85, "aa_cov": 0.34156378600823045, "hmm_cov": 0.8823529411764706, "evalue": 3.180227769809214e-13, "score": 45.12896728515625, "start": 48, "stop": 130 } ], "seq_stats": { "molecular_weight": 27315.34370000002, "isoelectric_point": 6.653595161437989 }, "id": "CNOCLNGIAL_275", "locus": "GCA000143945_00239" }, { "type": "cds", "sequence": "contig_1", "start": 137476, "stop": 138135, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03483.22" ], "nt": "TTGTTAATTAACCAGGAGTTACGTCCAGTCAACTGCTTTGTGGACCAGGCTAATATGGTAACTGTTTACACTGGTCAACCAATCCACTGCCATGATGCTGACAAGATCCATGGAAGTGTGGAACTTAAACTAGCCACCCAATTGGAAACGATGTTGGCCTTAGACAACAAGGAGTATGAGATTAAACCGGGTGATCTAGTGGTAGCTGATGAACAGGGCACAATTGCCATAGTCGGTATTATTGGTAGTAAGCGCACTATGGTCGACAATACGACCAACAACATCTTTTTTGAAGTTGTTAACTATAACCATGAGCGAATTAAGCAAACCGCTCAGCGTTTAGGAGTTGCTAACTTTGCTAGTAGGTTAATGAGTAAACCAATTAGTTTACAAGCTACCGAAAACTGCTTGAACTATTTGCAAAATAACTTCCTCAATCCCGAAAGCATTGGCAAGATTAGTAAGTTTTCCAGCACCATTAAAGCCCCAGCTTTTAACCGCAAAATTTACCTCAACTTTAATCAGTTACGGGAGTTAATTGGAGTGACTAAAAAGCAGTTAAATGACTACATGATCCGTAACTACCTTACTAGTTTAGGGTTCAAAATGGAAAACCAAATTGCCCGCGCCCCCGCTTACCGGCAAGACATTACGGTTTGA", "aa": "MLINQELRPVNCFVDQANMVTVYTGQPIHCHDADKIHGSVELKLATQLETMLALDNKEYEIKPGDLVVADEQGTIAIVGIIGSKRTMVDNTTNNIFFEVVNYNHERIKQTAQRLGVANFASRLMSKPISLQATENCLNYLQNNFLNPESIGKISKFSSTIKAPAFNRKIYLNFNQLRELIGVTKKQLNDYMIRNYLTSLGFKMENQIARAPAYRQDITV", "aa_hexdigest": "10ed1f56ab4a5036700e6912ac4ceb4e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03483.22", "name": "B3_4", "length": 143, "aa_cov": 0.6529680365296804, "hmm_cov": 1.0, "evalue": 5.280062062013168e-44, "score": 145.3409881591797, "start": 2, "stop": 144 } ], "seq_stats": { "molecular_weight": 24831.27690000002, "isoelectric_point": 8.692675209045408 }, "id": "CNOCLNGIAL_276", "locus": "GCA000143945_00240" }, { "type": "cds", "sequence": "contig_1", "start": 138154, "stop": 138540, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "TTGTTAAAGATCTTGGACCTCAACAAGATTAAAGAGGATGAAATTTTAAGCACCACTAAACTAGAGAAACACGAAAAACTCAATGCTTACGATGCGTTGCAAAAGCTACGCACTAAACTGCAAACCTTGGGCTTTCACAATGTGATTACTTACCAGTTAATTTCACCAGAACGAGCGCGTAACTTTAACCTGTTTGGGTTGAGTAATTTGTGGGAAATTAAAAACCCGCTTTCCAACGAACGGAGTGTTTTACGGGTTGGTTTAATTGACTCCTTGTTGCGAGTAATCCAAAAGAATGCCGCATACAAAAATAAGTTAGGCAACATCTTTGAGTTTTCTTTTGTCAAAACTAAGGACAGTAACCAACTCCACATTGCTGCTCTTTGA", "aa": "MLKILDLNKIKEDEILSTTKLEKHEKLNAYDALQKLRTKLQTLGFHNVITYQLISPERARNFNLFGLSNLWEIKNPLSNERSVLRVGLIDSLLRVIQKNAAYKNKLGNIFEFSFVKTKDSNQLHIAAL", "aa_hexdigest": "ca1e487d8471db22b18d8e51c488f62c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 111, "aa_cov": 0.8125, "hmm_cov": 1.0, "evalue": 6.512933333651652e-20, "score": 66.95671844482422, "start": 18, "stop": 121 } ], "seq_stats": { "molecular_weight": 14809.074999999983, "isoelectric_point": 9.733388328552245 }, "id": "CNOCLNGIAL_277", "locus": "GCA000143945_00241" }, { "type": "cds", "sequence": "contig_1", "start": 138550, "stop": 139149, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "ATGTTTGGTTCTACCTACCAAAAGGATCAAGGCGTTAGTGTCGATATTCCCGCCATGAAGGGCTTAGCGCAGTTAATTATTTCCAACTTTGGTTTTAACTGCGACTTTGAACCAATTACTGAAGGTGAGTACTTCACTAAGAACGTCGGTTTAAAACTAGTGGTGTTTAACGAACAGATTGGTTATGTTGGTTTAATTAAGGATGAGCTGTTGGCGCCTTATGATTTAAAGGGTAGACCGGTGTATGGTTTGGAAATTAACCTAGACCGCTTGTTGAACTCCTTAAACCGGTTGGAGCGCAGTTATACCCCGATTAGCAAACTACAAGATGTCTTTAAGGATATTACCTTTAGCTTCCCCCGTGATGAAAGTCACTTTGAAACGTTTGTGAAAGCGATTAAAAAACTACAAACGATTTTTAAATGGGAATTGATCTCGGTGTTTGACACCGAAAAGGATGGTGTACCAATCACCAAATATACAGTGCGCTATTACTTAAAGAATTTCACTAATACACCATTAACGTTAGAACAAATTAAAGCAGTGGAAACGCAGTTAAAACAACAGTGTGAACTAGCTAAAATAGCACTCGATCTTTAG", "aa": "MFGSTYQKDQGVSVDIPAMKGLAQLIISNFGFNCDFEPITEGEYFTKNVGLKLVVFNEQIGYVGLIKDELLAPYDLKGRPVYGLEINLDRLLNSLNRLERSYTPISKLQDVFKDITFSFPRDESHFETFVKAIKKLQTIFKWELISVFDTEKDGVPITKYTVRYYLKNFTNTPLTLEQIKAVETQLKQQCELAKIALDL", "aa_hexdigest": "3080ec9fa8f0eeee8f5be8ddf20281b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 81, "aa_cov": 0.4020100502512563, "hmm_cov": 0.9753086419753086, "evalue": 3.501733004613432e-09, "score": 31.887662887573242, "start": 9, "stop": 88 } ], "seq_stats": { "molecular_weight": 22883.141000000014, "isoelectric_point": 5.5789403915405265 }, "id": "CNOCLNGIAL_278", "locus": "GCA000143945_00242" }, { "type": "cds", "sequence": "contig_1", "start": 139262, "stop": 139570, "strand": "+", "frame": 2, "gene": "ytxK", "product": "putative protein MPN_107", "db_xrefs": [ "COG:COG0827", "COG:L", "RefSeq:WP_017532745.1", "SO:0001217", "UniParc:UPI0002F5B4F0", "UniRef:UniRef100_A0AB38W822", "UniRef:UniRef50_P75562", "UniRef:UniRef90_P75562" ], "nt": "ATGACAAACCAAAACCTACAAGACTTTCTGGACGGAGTTTTGTTAAAAGAGGAAAATTTAAACAAAATTACTGAAGTTTCCCTAGAAAAGGGATTACCGCACCTTTTAGATGAGGGTATCCATAACGAAGATAACTTTCAGATCTTTACGAGCCAAAAGATTGTGGCAGACATGATCAAAATGGTCGGTGTAAAGGAAATGAAAAATGTCCACACTACTGTTTTAGAACCAACTAGTGGTGATGGTGCTTTTACTTGTGCTATTTTGGATTTACGTTTAAAAAACATTAAAAAATCAGACAGCTTTTAA", "aa": "MTNQNLQDFLDGVLLKEENLNKITEVSLEKGLPHLLDEGIHNEDNFQIFTSQKIVADMIKMVGVKEMKNVHTTVLEPTSGDGAFTCAILDLRLKNIKKSDSF", "aa_hexdigest": "ac62112c20b7afaa3f91ff1abf6b405f", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI0002F5B4F0", "ncbi_nrp_id": "WP_017532745.1", "uniref100_id": "UniRef100_A0AB38W822", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F5B4F0", "RefSeq:WP_017532745.1", "UniRef:UniRef100_A0AB38W822" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W822", "uniref90_id": "UniRef90_P75562" }, "psc": { "uniref90_id": "UniRef90_P75562", "gene": "YtxK", "product": "Uncharacterized protein MPN_107", "uniref50_id": "UniRef50_P75562", "cog_id": "COG0827", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_P75562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75562" ], "product": "Uncharacterized protein MPN_107" }, "genes": [ "ytxK" ], "id": "CNOCLNGIAL_279", "locus": "GCA000143945_00243" }, { "type": "cds", "sequence": "contig_1", "start": 139624, "stop": 140016, "strand": "+", "frame": 1, "gene": null, "product": "Restriction methylase, adenine-specific", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031E8CE1", "UniRef:UniRef100_A0AB38WB52", "UniRef:UniRef50_A0AB38WB52", "UniRef:UniRef90_A0AB38WB52" ], "nt": "TTGGTTTGGTTACAACGCAATAACCTTTACTCTATCTTTTTAGCCTTCTTAAAAAAGCACGGTATTTTTAACGAAGAGTTAAGTCTCTTAGTGAAAAAAATCTTGATGATTAATGTGAGTTGGGGCGAGTTAATCACAGAAACCAAAGATAACGAGAACTTCGTTTGTAACTACGATTATTTTTTAAGGAATAAAAAGACAAAGAAAAAACCCGCAAAGCATAAACATGAATTTCCAGAGGGTTTATTTTCCAATGGAGAAGGTAAGTGCAAAAGTTTGTTTGTTAATTGGTCATTTGCTGATGTCATTAATGAAATTGCTCATAATTCCAATTTTGACTTTGCCAAAATAGCAAAGATACCTGGTGAAATAATTCAATTTCAAATTAATTAG", "aa": "MVWLQRNNLYSIFLAFLKKHGIFNEELSLLVKKILMINVSWGELITETKDNENFVCNYDYFLRNKKTKKKPAKHKHEFPEGLFSNGEGKCKSLFVNWSFADVINEIAHNSNFDFAKIAKIPGEIIQFQIN", "aa_hexdigest": "7a00d55a9325a8b3b073ad6780758031", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00031E8CE1", "uniref100_id": "UniRef100_A0AB38WB52", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031E8CE1", "UniRef:UniRef100_A0AB38WB52" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38WB52", "uniref90_id": "UniRef90_A0AB38WB52" }, "psc": { "uniref90_id": "UniRef90_A0AB38WB52", "product": "Restriction methylase, adenine-specific", "uniref50_id": "UniRef50_A0AB38WB52" }, "pscc": { "uniref50_id": "UniRef50_A0AB38WB52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38WB52" ], "product": "Restriction methylase, adenine-specific" }, "genes": [], "id": "CNOCLNGIAL_280", "locus": "GCA000143945_00244" }, { "type": "cds", "sequence": "contig_1", "start": 140018, "stop": 140164, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTCAAAATGGCGATAAAGACCACGAATTGATTCAGGGAAAGGTCAAAAGCAAAACTGAAATAGAAGATTCCGTTATATTGGATCAAGACACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGA", "aa": "MSQNGDKDHELIQGKVKSKTEIEDSVILDQDTLQGKGEEKTKLDELIR", "aa_hexdigest": "f3f5d9b52d27eb5d5157aa0bf9fa26b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5440.999399999999, "isoelectric_point": 4.749886894226075 }, "id": "CNOCLNGIAL_281", "locus": "GCA000143945_00245" }, { "type": "cds", "sequence": "contig_1", "start": 140372, "stop": 140554, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAAGTTATACCGGATGAATATGAACGCAAGAAACACATCATGACCAAGATGCTTTTTAGTATTGACTTAACTAAATTTACTAGCTTGATGGTTCGTCGTACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAACTAGTGAAGATGAGGGCTGTGCAATTGCAAATGGCGCATGA", "aa": "MAKVIPDEYERKKHIMTKMLFSIDLTKFTSLMVRRTLYYCIKANKRKTSEDEGCAIANGA", "aa_hexdigest": "13a6ddf81ceaae4e007af63f4d0b1fd7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6960.175300000001, "isoelectric_point": 9.468423271179201 }, "id": "CNOCLNGIAL_282", "locus": "GCA000143945_00246" }, { "type": "cds", "sequence": "contig_1", "start": 140523, "stop": 140714, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGGCTGTGCAATTGCAAATGGCGCATGATTTAATAACGAATGCGGTAATGTTGTTAAACCTTACAAGGAACATTATTTCGACAAACAAGGAAAAAATAAAAAATGTAAGTTTTGTAGAACAGATGAGAAATTTAAATACCAACAATCTTCTTCAAATGAAAAATACGCCTATGACTTCATTCATTTAA", "aa": "MRAVQLQMAHDLITNAVMLLNLTRNIISTNKEKIKNVSFVEQMRNLNTNNLLQMKNTPMTSFI", "aa_hexdigest": "0fdad38dbf56bf509e89c74389e95626", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7290.559700000002, "isoelectric_point": 10.432676887512205 }, "id": "CNOCLNGIAL_283", "locus": "GCA000143945_00247" }, { "type": "cds", "sequence": "contig_1", "start": 140759, "stop": 140923, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07669.17" ], "nt": "ATGAAATTTGATGTAATTATTGGTAATCCACCTTATCAGTTAGCAAATAATCGTGGTGGTGATGCTGGTGATGGTAATGGAGCAAACCCCATTTTTCAAGAATTTATCTTAAGAGCACTTGAATTACAACCAAAATATTTAGCAATGATTGTTCCTGCAAGATGA", "aa": "MKFDVIIGNPPYQLANNRGGDAGDGNGANPIFQEFILRALELQPKYLAMIVPAR", "aa_hexdigest": "0be93b2c684b66dce19192d4acd70eb0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07669.17", "name": "Eco57I", "length": 65, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.751925786629206e-14, "score": 49.69576644897461, "start": 1, "stop": 54 } ], "seq_stats": { "molecular_weight": 5915.756799999999, "isoelectric_point": 6.0008562088012685 }, "id": "CNOCLNGIAL_284", "locus": "GCA000143945_00248" }, { "type": "cds", "sequence": "contig_1", "start": 141561, "stop": 141812, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2B7000", "UniRef:UniRef90_UPI00132F963A" ], "nt": "ATGCCCAGAATAACTGGTAGCGGTGTGTTTGGTGATTACAGACCCAATATAAAAATTGTTGAACCTACTGGAGTTTGCACTCATAGTTATGTAGTTGCTTATTTAACGACAAATAAGTTTGAAGTTGAAAATGTCTTTTTATACATGAAAACTAAATTTTTTAGATTTTTTGTCGAGATTTTTAAAGCAACCATCAATATCAGTTCACATAACTTCAAATACGTTCCCATTCAAGATTTTTCCCGTCCTTGA", "aa": "MPRITGSGVFGDYRPNIKIVEPTGVCTHSYVVAYLTTNKFEVENVFLYMKTKFFRFFVEIFKATINISSHNFKYVPIQDFSRP", "aa_hexdigest": "836e99bdcc47ec468f96fde1a0b7ec41", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 141561, "stop": 141899, "observations": { "mutation": [ 141810, 141858 ], "stop": [ 141810, 141858 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00132F963A", "query_cov": 1.0, "subject_cov": 0.7410714285714286, "identity": 0.988, "score": 168.0, "evalue": 1.29e-52, "gene_start": 1, "gene_end": 83, "reference_start": 1, "reference_end": 83, "reference_sequence": "MPRITGSGVFGDYIPNIKIVEPTGVCTHSYVVAYLTTNKFEVENVFLYMKTKFFRFFVEIFKATINISSHNFKYVPIQDFSRPWNDRELYQKYNLTQEEWQYIENNISSYEN" }, "paralog": false, "identity": 0.9732142857142857, "score": 223.0, "evalue": 1.07e-76, "description": "Internal stop codon at 141810, 141858. Nonsense mutation around 141810, 141858.", "psc": { "uniref90_id": "UniRef90_UPI00132F963A", "uniref50_id": "UniRef50_UPI001F2B7000", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00132F963A", "UniRef:UniRef50_UPI001F2B7000" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2B7000", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2B7000" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9694.13059999999, "isoelectric_point": 9.415430259704593 }, "id": "CNOCLNGIAL_285", "locus": "GCA000143945_00249" }, { "type": "cds", "sequence": "contig_1", "start": 142457, "stop": 142618, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGAAAGATACTTTCAAGCCAATCCCGAAAACGGTAGGTTTTTAATGAACTGCAAAATGCGGTTCGGTAAATGTTTCACCTTGTACTCTTATGCACAAAAGAATAACATTAACAAGATTTTGATCCTAACGTTTGTTCCAGCTGTCGAAGAATCTTGA", "aa": "MLERYFQANPENGRFLMNCKMRFGKCFTLYSYAQKNNINKILILTFVPAVEES", "aa_hexdigest": "874a998ad47ce4006dee46d92458f397", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6283.305899999999, "isoelectric_point": 9.138022804260256 }, "id": "CNOCLNGIAL_286", "locus": "GCA000143945_00250" }, { "type": "cds", "sequence": "contig_1", "start": 142673, "stop": 143677, "strand": "+", "frame": 2, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HLI1", "UniRef:UniRef90_UPI0036F40F46" ], "nt": "TTGCAAAAATCTAATTTTGATTTAAAGAATCAAAACGAGCCTTATGTAGTCTTTTTATCACTGCAAAACGTCTTAGGTAAACAAAGAATAGATGGCGCCAAAACTGATTTTGATAAAGAGCGAATTAGTAAGCTACAAGAGATTGATTTTGATTTAATTGTTTTTGATGAGTACCACTACGGAGCCAACAAAAAACGAACCCAAATCAAAGTTGAAAAAGTCAATAAAAAGATTGATAATCCAGAACAGCAAGATAATCAAGATGATGCTGAGGAAGAATTGGCAAGTACTTTTAAGTTAAAAGACATCAAAAGTAAATTTCAATTTTCATACAAACAATTAGTTTGTCTGTCAGGTACTCCTTTTTCCTCTTTAAGAAATAATGAGTTTTCCAGTAAAGATCAGGTGTTTACTTATTCTTACTTTGATGAACAGAAGGCCAAATCAGCAGAAAATCACCCACTCAAATTAGGGCAATACGGAATATTTCCGGAAATGAATATTTACTGTTTTGAATTGGCGGAAATTTTTACTGCACAAGAACAAGAAATATTCATAACTCCAGGTAAAGGTAAAAATAAGTTACCTGAAATTAGTTTTCGAAAGCTGTTTCAAACGGAAAACGTTTCTAAAGAATCAAGCAAACCAGTTTACAGATTTGTAAATGAAAACCTGGTTGAAAAGCTTATAGATTCTTTAATTGATAAACGTAAAGGTTTTAGCCACACACCTTTAAGTTGGGAAAACATTGACAAGCATAAGCATTCCTTATTAATTTTGCCAACTAGAGTTGCTTGTTTTGCGTTGGCCAATTTACTTAAAAATCACTGGTACTTTGAAAACAATGATTTTCAAATTATCAACATGTCAGAGAGCCAGTTTGGTAATGGCAAAAAAGCTTTAATTGAGCTTAATAAACACTTAGATGAAGCTAAAAAGACTAATAAAAATACTTTAACTATTACTGTAGCTAAATTAACTATTGGTATTACAGTTAAAGAGTGA", "aa": "MQKSNFDLKNQNEPYVVFLSLQNVLGKQRIDGAKTDFDKERISKLQEIDFDLIVFDEYHYGANKKRTQIKVEKVNKKIDNPEQQDNQDDAEEELASTFKLKDIKSKFQFSYKQLVCLSGTPFSSLRNNEFSSKDQVFTYSYFDEQKAKSAENHPLKLGQYGIFPEMNIYCFELAEIFTAQEQEIFITPGKGKNKLPEISFRKLFQTENVSKESSKPVYRFVNENLVEKLIDSLIDKRKGFSHTPLSWENIDKHKHSLLILPTRVACFALANLLKNHWYFENNDFQIINMSESQFGNGKKALIELNKHLDEAKKTNKNTLTITVAKLTIGITVKE", "aa_hexdigest": "eb37f6922c49439d0dff3c7c5fcd5a7a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 142374, "stop": 143623, "observations": { "deletion": [ 142407 ], "stop": [ 142617 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0036F40F46", "query_cov": 0.9461077844311377, "subject_cov": 0.7348837209302326, "identity": 0.997, "score": 624.0, "evalue": 7.11e-224, "gene_start": 1, "gene_end": 316, "reference_start": 113, "reference_end": 428, "reference_sequence": "MKIRISLNILAKFLDSFAHLLFLKNKEIYTPRKEQAACVEVLERYFQANPENGRFLMNCKMRFGKCFTLYSYAQKNNINKILILTFVPAVEESLKDDLNHIEKNYKFYTDDDLQKSNFDLKNQNEPYVVFLSLQNVLGKQRIDGAKTDFDKERISKLQEIDFDLIVFDEYHYGANKKRTQIKVEKVNKKIDNPEQQDNQDDAEEELASTFKLKDIKSKFQFSYKQLVCLSGTPFSSLRNNEFSSKDQVFTYSYFDEQKAKSAENHPLKLGQYGIFPEMNIYCFELAEIFTAQEQEIFITPGKGKNKLPEISFRKLFQTENVSKESSKPVYRFVNENLVEKLIDSLIDKRKGFSHTPLSWENIDKHKHSLLILPTRVACFALANLLKNHWYFENNDFQIINMSESQFGNGKKALIELNKHLDEAKKTNKIL" }, "paralog": false, "identity": 0.9951923076923077, "score": 833.0, "evalue": 2.01e-308, "description": "Internal stop codon at 142617. Frameshift due to deletion around 142407.", "psc": { "uniref90_id": "UniRef90_UPI0036F40F46", "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_A0AB33HLI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0036F40F46", "UniRef:UniRef50_A0AB33HLI1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HLI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HLI1" ] } }, "id": "CNOCLNGIAL_287", "locus": "GCA000143945_00251" }, { "type": "cds", "sequence": "contig_1", "start": 143693, "stop": 143881, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGGATTTAAAGGGTGCTGAATCTTATTTCCAAACGATTTTTAGAATCCAAACTCCATATATAAAGAACTGTAAAAATCTGAAAGAAATTTGTTATGTTTACGACTTCAACATGTATAGGTGTTTGGAAGTTACTAACGAGTATTCAAAACAAACACAAACAGACCCTAAATTTAGTGCTAGTTGA", "aa": "MKDLKGAESYFQTIFRIQTPYIKNCKNLKEICYVYDFNMYRCLEVTNEYSKQTQTDPKFSAS", "aa_hexdigest": "793492b363d0808a3826746ea9fc44b2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7435.424700000002, "isoelectric_point": 8.458783912658692 }, "id": "CNOCLNGIAL_288", "locus": "GCA000143945_00252" }, { "type": "cds", "sequence": "contig_1", "start": 144011, "stop": 144256, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAAAGTAACATTATCGACATAGATGTACTTTGCAATGTTGGGCAAGATGAAGATGCGCAAAAAATATTGAAAAAAATATTAGCTCATAAAAAATTTAAATCATCCAAAAAGAAGCGAGAGTTTGAAGACGTTCATTTGGAAAAAAGTCCAAAAAGTGAGGCATTTAGTGAAGGCGTTCGCTCTGGTAAGGATTACGCCGCTGAACAAGGTAATGTTTTAGAAGAACTTGAAAATTTCTGA", "aa": "MDESNIIDIDVLCNVGQDEDAQKILKKILAHKKFKSSKKKREFEDVHLEKSPKSEAFSEGVRSGKDYAAEQGNVLEELENF", "aa_hexdigest": "ffba8b18e2dc67ab777a0ede53ab4c82", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9226.243599999996, "isoelectric_point": 5.168903923034666 }, "id": "CNOCLNGIAL_289", "locus": "GCA000143945_00253" }, { "type": "cds", "sequence": "contig_1", "start": 144399, "stop": 144566, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCAGAATAAAATCAAAGACTTTAAAAAAAGAAAAGGAAGAACAACTTCAACTTTTAAAAAATGAAAATGGTTATATTTCTGATGAGAATAACTTAAAAAAGCTAAGAGATTTAATTAAAGAATATGTACAAAACAGAAGCAACATACCTCCAGAATTAGACTGA", "aa": "MFRIKSKTLKKEKEEQLQLLKNENGYISDENNLKKLRDLIKEYVQNRSNIPPELD", "aa_hexdigest": "1eecdc65706310bc9296577b921531cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6633.5424, "isoelectric_point": 9.003348350524902 }, "id": "CNOCLNGIAL_290", "locus": "GCA000143945_00254" }, { "type": "cds", "sequence": "contig_1", "start": 144693, "stop": 144947, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU34", "UniRef:UniRef90_A0AB33HU34" ], "nt": "ATGCATACATGGGCACGTGTTCTGCACTTTCTTTATGTATCTGTTTATAGAGAAAAAACTTTAAGAGATGTGTTGAATAAAACTGATCCCAAAGTTTTTTCAGTGGCAGTCGGAATTGAAATGGATGAGTTTTCCAAAATCCAAAAATTTTTTAAAGTGGAAAATATGAATAGACTCATCGAACAGTTTAGTTTTAGAGAAAGTGTTAAAACTGAAAACACAGAAAAATCACAGTATAGAAACAGTTTCAATTGA", "aa": "MHTWARVLHFLYVSVYREKTLRDVLNKTDPKVFSVAVGIEMDEFSKIQKFFKVENMNRLIEQFSFRESVKTENTEKSQYRNSFN", "aa_hexdigest": "e12e6933ae29fb8dd708477d305bc870", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 144399, "stop": 144980, "observations": { "mutation": [ 144564, 144945 ], "stop": [ 144564, 144945 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HU34", "query_cov": 1.0, "subject_cov": 0.43523316062176165, "identity": 1.0, "score": 166.0, "evalue": 9.79e-51, "gene_start": 1, "gene_end": 84, "reference_start": 99, "reference_end": 182, "reference_sequence": "LFRIISKTLKKEKEEQLQLLKNENGYISDENNLKKLRDLIKEYVQNRSNIPPELDWFFYRDRFIQLFEKSCYQHFKKKTLTEEQKEKKKKVNDNFDSLMHTWARVLHFLYVSVYREKTLRDVLNKTDPKVFSVAVGIEMDEFSKIQKFFKVENMNRLIEQFSFRESVKTENTEKSQYRNSFNWFGFGPDSLKS" }, "paralog": false, "identity": 0.9844559585492227, "score": 379.0, "evalue": 6.65e-137, "description": "Internal stop codon at 144564, 144945. Nonsense mutation around 144564, 144945.", "psc": { "uniref90_id": "UniRef90_A0AB33HU34", "uniref50_id": "UniRef50_A0AB33HU34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HU34", "UniRef:UniRef50_A0AB33HU34" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HU34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU34" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10157.494499999995, "isoelectric_point": 9.344708442687988 }, "id": "CNOCLNGIAL_291", "locus": "GCA000143945_00255" }, { "type": "cds", "sequence": "contig_1", "start": 144997, "stop": 145143, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAATAGTGGTAAAGACTACGAATCGATTCAAGGTAAGATTAAGAGTAAAGCTGAAATAGAAGATTCAGTTATATTGGAAAAAGAAACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGA", "aa": "MNKNSGKDYESIQGKIKSKAEIEDSVILEKETLQGKGEEKTKLDELIR", "aa_hexdigest": "49cd77569ed271ca55b0f3753a38abbd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5452.108199999999, "isoelectric_point": 5.364145851135253 }, "id": "CNOCLNGIAL_292", "locus": "GCA000143945_00256" }, { "type": "cds", "sequence": "contig_1", "start": 145396, "stop": 145893, "strand": "+", "frame": 1, "gene": null, "product": "Eco57I restriction-modification methylase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132FCD8E", "UniRef:UniRef90_UPI00133023F0" ], "nt": "ATGACCAAGATGCTTTTTAGCATTGGCTTAACTAAATTTACTAGTTTGATGGTGCGTCGCACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAATCAGCGAAGATGAGGGTTGTGCAATTGCAAATGGCGCACGATTTAATAATGAATTTGGCAATGTTGTTACCCCTTATAAAGAACACTATTTTGGAAAAGAATCGAAAAGCAAAAACTGTCACTTTTGTGGGACAAATAAGAATTCTAAATATGTAAATTCTATGACTGAAGAAAAGTATGCCTATGACTTCATTCATTTAAATTCGGATGAATATGAAAACTACTTTAAAACTAATTTTGGTGTTATGAAATTTGATGTAATTATTGGAAATCCGCCTTACCAGCTATCTAATGGTAGCGGTTCTGATGGCAATGGAGCCAAAGCAATTTTTCAAGATTTTGTTTTAAAAGCAATTGACTTAGAACCAAAATATTTAGCGATGATTATCCCAGCAAAATGA", "aa": "MTKMLFSIGLTKFTSLMVRRTLYYCIKANKRKISEDEGCAIANGARFNNEFGNVVTPYKEHYFGKESKSKNCHFCGTNKNSKYVNSMTEEKYAYDFIHLNSDEYENYFKTNFGVMKFDVIIGNPPYQLSNGSGSDGNGAKAIFQDFVLKAIDLEPKYLAMIIPAK", "aa_hexdigest": "c9304ab361307b4525c7e6e2730f9ee2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 145096, "stop": 145931, "observations": { "deletion": [ 145909 ], "mutation": [ 145141, 145264, 145285, 145891 ], "stop": [ 145141, 145264, 145285, 145891 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00133023F0", "query_cov": 1.0, "subject_cov": 0.532258064516129, "identity": 0.9940000000000001, "score": 334.0, "evalue": 4.45e-114, "gene_start": 1, "gene_end": 165, "reference_start": 134, "reference_end": 298, "reference_sequence": "MNKNSGKDYESIQGKIKSKAEIEDSVILEKETFQGKGEEKTKLDELIRWEAEKNDLLKLIDNVGNDEVFTPVETCQRMLDELFPEDHEVWSNPDLKWLNPCDKNGVFFREIALRLDKGLTKIIPDEYARKKHIMTKMLFSIGLTKFTSLMVRRTLYYCIKANKRKTSEDEGCAIANGARFNNEFGNVVTPYKEHYFGKESKSKNCHFCGTNKNSKYVNSMTEEKYAYDFIHLNSDEYENYFKTNFGVMKFDVIIGNPPYQLSNGSGSDGNGAKAIFQDFVLKAIDLEPKYLAMIIPAKWMISAKYFFKSK" }, "paralog": false, "identity": 0.9784172661870504, "score": 542.0, "evalue": 2.92e-198, "description": "Internal stop codon at 145141, 145264, 145285, 145891. Frameshift due to deletion around 145909. Nonsense mutation around 145141, 145264, 145285, 145891.", "psc": { "uniref90_id": "UniRef90_UPI00133023F0", "product": "Eco57I restriction-modification methylase domain-containing protein", "uniref50_id": "UniRef50_UPI00132FCD8E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00133023F0", "UniRef:UniRef50_UPI00132FCD8E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00132FCD8E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132FCD8E" ], "product": "Eco57I restriction-modification methylase domain-containing protein" } }, "id": "CNOCLNGIAL_293", "locus": "GCA000143945_00257" }, { "type": "cds", "sequence": "contig_1", "start": 145894, "stop": 146181, "strand": "+", "frame": 1, "gene": null, "product": "Eco57I restriction-modification methylase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3844D4", "UniRef:UniRef90_UPI001F3844D4" ], "nt": "ATGATTTCTGCTGAAAATATTTTTTTAAATCTAAGAGATAAATTAAAGAAAAATAAAGGTATAAAAGAAATTAATATTTTTTTCGACTCTAAAGACTGTTTTCCAAAGAGAGAAATAAAAGGTGGCATTTGTTACTTTATCTGGCAGAATAACTATCAAGGTAAGACTCTTATAAATACCAAATTTTCAAAGAAAGGAAAAAGCACAGAGATCGATAGTTCTAAAAGAAACTTATTTGTTTCCGTTTTAAACAACACTGAACAAATCATTTTTCGCAAAAGGATTTGA", "aa": "MISAENIFLNLRDKLKKNKGIKEINIFFDSKDCFPKREIKGGICYFIWQNNYQGKTLINTKFSKKGKSTEIDSSKRNLFVSVLNNTEQIIFRKRI", "aa_hexdigest": "55bb0d021f9cc803407b3e29c114b6ed", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 145909, "stop": 146256, "observations": { "mutation": [ 146179 ], "stop": [ 146179 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F3844D4", "query_cov": 0.9473684210526315, "subject_cov": 0.7627118644067796, "identity": 0.978, "score": 179.0, "evalue": 1.38e-56, "gene_start": 6, "gene_end": 95, "reference_start": 1, "reference_end": 90, "reference_sequence": "NNFLNLRDKLKKNKDIKEINIFFDSKDCFPKREIKGGICYFIWQNNYQGKTLINTKFSKKGKSTEIDSSKRNLFVSVLNNTEQIIFRKRIWQDIYTKIDSISASEMKNTHTEREQFRS" }, "paralog": false, "identity": 0.9565217391304348, "score": 223.0, "evalue": 2.79e-76, "description": "Internal stop codon at 146179. Nonsense mutation around 146179.", "psc": { "uniref90_id": "UniRef90_UPI001F3844D4", "product": "Eco57I restriction-modification methylase domain-containing protein", "uniref50_id": "UniRef50_UPI001F3844D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F3844D4", "UniRef:UniRef50_UPI001F3844D4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F3844D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3844D4" ], "product": "Eco57I restriction-modification methylase domain-containing protein" } }, "id": "CNOCLNGIAL_294", "locus": "GCA000143945_00258" }, { "type": "cds", "sequence": "contig_1", "start": 146352, "stop": 146822, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_026088310.1", "SO:0001217", "UniParc:UPI0004755848", "UniRef:UniRef100_A0AB38WA20", "UniRef:UniRef50_A0AB38WA20", "UniRef:UniRef90_A0AB38WA20" ], "nt": "TTGAAAGCAGAAAAATTTCCCGGTTTGATTGCAATTTATGGAAGTCATTCGGTTATTAAACACGTGGATATTAAAGATATTCCGCAAATAAAAAATAATGAAGAAATTGCTAAATACAAAATTATTGTTCCACACGTTTATAGCCGTGGCAATGGAACTTTTGGAAATTACAAACCAAAAGTAAAAATTATTAAACCTAATGAAATTTGCACCGAAACTTATTTAGTGGTATATCCCACCGAAAGTAAAGTTGAGATTGAAAACGTCGCTTCTTATATGAGAACTAAATTTTTCAGATTTTTAGTTGAAATTTTTAAGGATTCTATTAACACAAATTCTCAAAATTTCAAGTTTATTCCTCTACAAGATTTTTCACGTCCTTGGAACGATAGAGAACTTTATGAAAAATACGGTTTAACACTCGAAGAACAGCAATACATCGAGGCGAACATAAGTGCTTACGAGGACTAA", "aa": "MKAEKFPGLIAIYGSHSVIKHVDIKDIPQIKNNEEIAKYKIIVPHVYSRGNGTFGNYKPKVKIIKPNEICTETYLVVYPTESKVEIENVASYMRTKFFRFLVEIFKDSINTNSQNFKFIPLQDFSRPWNDRELYEKYGLTLEEQQYIEANISAYED", "aa_hexdigest": "c0716801b80d9d4d8bec1f38971cef67", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0004755848", "ncbi_nrp_id": "WP_026088310.1", "uniref100_id": "UniRef100_A0AB38WA20", "db_xrefs": [ "SO:0001217", "UniParc:UPI0004755848", "RefSeq:WP_026088310.1", "UniRef:UniRef100_A0AB38WA20" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38WA20", "uniref90_id": "UniRef90_A0AB38WA20" }, "psc": { "uniref90_id": "UniRef90_A0AB38WA20", "uniref50_id": "UniRef50_A0AB38WA20" }, "pscc": { "uniref50_id": "UniRef50_A0AB38WA20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38WA20" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18312.711499999994, "isoelectric_point": 6.843323707580566 }, "id": "CNOCLNGIAL_295", "locus": "GCA000143945_00259" }, { "type": "cds", "sequence": "contig_1", "start": 147129, "stop": 147521, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_112", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874469.1", "SO:0001217", "UniParc:UPI000013A1CB", "UniRef:UniRef100_P75450", "UniRef:UniRef50_P75450", "UniRef:UniRef90_P75450" ], "nt": "ATGCTAGATAAATTACTCCAGAAATTTCGAGACCAAAAGAAGCCCGTCTTCCACAAAGAAGAGGGCTACTGGGAAATTAGTGCCTTGCGGAAATGGGCAGCCATTTTAATAATTGCCTTTGGAGCTGGCATTATTTACATTGTGCCCTACTTTGCCTTCTTTCAGTTTAAGACAGCAGTAGCTAATGTAACGGGAGTGGAGCCCAACAGAATCTCCCTTCTCTTAACTGCTTACGGGATTGTTTCATTGCTGTTTTATATTCCTGGTGGTTGGTTAGCTGACCGGATTTCAGCTAAAGCGCTGTTTTCGGTATCAATGTTTGGTACTGGCATTATTACCTTCTGGTACTTCTTAGTTGGTTTGAAGGGCATAGTGTGGATTACACCCAACTAG", "aa": "MLDKLLQKFRDQKKPVFHKEEGYWEISALRKWAAILIIAFGAGIIYIVPYFAFFQFKTAVANVTGVEPNRISLLLTAYGIVSLLFYIPGGWLADRISAKALFSVSMFGTGIITFWYFLVGLKGIVWITPN", "aa_hexdigest": "09afb655a9cf37dc9cec4897c8f01479", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A1CB", "ncbi_nrp_id": "WP_010874469.1", "uniref100_id": "UniRef100_P75450", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A1CB", "RefSeq:WP_010874469.1", "UniRef:UniRef100_P75450" ] }, "ips": { "uniref100_id": "UniRef100_P75450", "uniref90_id": "UniRef90_P75450" }, "psc": { "uniref90_id": "UniRef90_P75450", "product": "Uncharacterized protein MPN_112", "uniref50_id": "UniRef50_P75450", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75450", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75450" ], "product": "Uncharacterized protein MPN_112" }, "genes": [], "id": "CNOCLNGIAL_296", "locus": "GCA000143945_00260" }, { "type": "cds", "sequence": "contig_1", "start": 147503, "stop": 147991, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTACACCCAACTAGTTATTATCCACGTCCTGTATGCCCTTAGTACGGTCTTAACCTTCTGGAGTGCCTTCATTAAAAGTATTAAGTTATTGGGTCCAGCGGATGAACAAGCGAGGTTGTATTCCACATCTGAAGGATCACGCTACTTAATCCAAGTAATTACTGGTTACATTAGTGCTGGTTTGGGTGCTATTGTCCTTATTAGTACTCCCTTTCTCATTTACAAAGCACCAACTGCTGCTGACCCTAAAAACTTAGCTTCTTCCTCAGGGATCTTCTTTGCCTTACTGTTTTATGCCATTTGTTATGTGTTAGCTGGTTTACTGTCACTGTTTTTTTTACCAGGAAAGTTCTGGGAAAAGCGCAAGATTTACTTGGAAGATGAACGAATTGAATACTTTAACCCTGTGGACCAGGCTTATCACATCTTTAAATCGGAAGCAGAATTAGAAGCCTTTAAGAAACATAACACTAAGGTTTTTTGA", "aa": "MDYTQLVIIHVLYALSTVLTFWSAFIKSIKLLGPADEQARLYSTSEGSRYLIQVITGYISAGLGAIVLISTPFLIYKAPTAADPKNLASSSGIFFALLFYAICYVLAGLLSLFFLPGKFWEKRKIYLEDERIEYFNPVDQAYHIFKSEAELEAFKKHNTKVF", "aa_hexdigest": "e6a77491f07f2ef6e2a526797d67a3f0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18355.18789999999, "isoelectric_point": 7.786636543273926 }, "id": "CNOCLNGIAL_297", "locus": "GCA000143945_00261" }, { "type": "cds", "sequence": "contig_1", "start": 148070, "stop": 148354, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTGTACCAAACGATTGCTAACTTTGGTACTAACATTTTGGAATCGCTAGGACTAGAGGCAGCACTCTCCCCAACTTTAAGTAATACCTATATCTATGGGATTCCGGTGTTAGGGGCAATCTTTTCTGGTTTGATTAACAAAAAGTTAACTAAATCAACCGCTAAAAGTCTGATTGTGCAAGCCTTGTTTGTCATTTTGGGTACCTTAGCCTTTTTAGTAATAACACTAGCTTCCCCAGCCAAACCAGAAACTGGTGCATTTAACCAAGCTACCTTGTGA", "aa": "MNVYQTIANFGTNILESLGLEAALSPTLSNTYIYGIPVLGAIFSGLINKKLTKSTAKSLIVQALFVILGTLAFLVITLASPAKPETGAFNQATL", "aa_hexdigest": "c62f5efa1006dbd723ea99eb0a027294", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9873.500899999988, "isoelectric_point": 9.158330345153807 }, "id": "CNOCLNGIAL_298", "locus": "GCA000143945_00262" }, { "type": "cds", "sequence": "contig_1", "start": 148382, "stop": 148600, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTATTGGCGCGAACCGTTCCATTTTTTGGAGTACGATCACAGAACTGAAAGTCAATAAGGAAATAGTAGGACTGGCAGTTGGTTTTATTTCGATTATTGGTTTTTCCAAAGATGTGTGGTTAAGTCCCTTACTGACTGGTACTACTAACCAGTTCATTGTCAAAAACAGTCAGGGTACCTCGTTTTACAGCCAGCAAGCCTTGGTAGCCTGA", "aa": "MFFIGANRSIFWSTITELKVNKEIVGLAVGFISIIGFSKDVWLSPLLTGTTNQFIVKNSQGTSFYSQQALVA", "aa_hexdigest": "7ad1448c6ae1df7dfa260762d181f4f9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7932.1071999999995, "isoelectric_point": 9.398732948303223 }, "id": "CNOCLNGIAL_299", "locus": "GCA000143945_00263" }, { "type": "cds", "sequence": "contig_1", "start": 148731, "stop": 148880, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACACTAAATTAACACCACCCTGGTTGCGTCAAGAAAATAACCTGCTCAACCCCGAAGTTACCCAACGTTTGTTTATCAACCAGGACAACATTCCGAACTTACCCACTGAAGCCCCCCAAGTTTATTTAGCCCGTTTTTTGGAATGA", "aa": "MDTKLTPPWLRQENNLLNPEVTQRLFINQDNIPNLPTEAPQVYLARFLE", "aa_hexdigest": "d35db3a995426340778277bb19f514a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5792.5352, "isoelectric_point": 4.800644111633301 }, "id": "CNOCLNGIAL_300", "locus": "GCA000143945_00264" }, { "type": "cds", "sequence": "contig_1", "start": 148911, "stop": 149114, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGCACAAGCAGCAGTTCAGGATTACCTCAATTCTAAAGCCTGTGCACAAATTGAAACCATTATTGCAGAACGGGCGCAAAACACCCATTCGAGTTGGTTAGCTAACTGGTGGGTGCAGTACGCTTATTTAACTTCGACTGGCCCGGTTAGTCCGGAAGTTAATGCACCCTATTATTTAGAGCTCCCGACTGTTGGTTGA", "aa": "MKAQAAVQDYLNSKACAQIETIIAERAQNTHSSWLANWWVQYAYLTSTGPVSPEVNAPYYLELPTVG", "aa_hexdigest": "b09559ff02a9e4e227171cbef723aa7f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7460.283500000001, "isoelectric_point": 4.957690238952636 }, "id": "CNOCLNGIAL_301", "locus": "GCA000143945_00265" }, { "type": "cds", "sequence": "contig_1", "start": 149232, "stop": 149507, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATCAATCTTTGCTAGTTGCCAGATTCACCGCGCTGATGGGGATGTGTACTTTGTTAATGATCAACCTGCTAACTTCATTGTGGTGATTAAGAACAATGTCTTCTATAAGTTAGTAATAGATAACAGTGGTAGCTTAGAACAACTGCAAGCGCAACTGCAGTTAAGCTTTGTGCAAATCTTAGACAATGAGCTAAGTCACCCACCGCACTGGAACTTACTCACTGCTACCACAACCAAAGCGGAGTCACAAAGTTTACTCGATCAGTTGTGA", "aa": "MQSIFASCQIHRADGDVYFVNDQPANFIVVIKNNVFYKLVIDNSGSLEQLQAQLQLSFVQILDNELSHPPHWNLLTATTTKAESQSLLDQL", "aa_hexdigest": "e3f5d7731d015054c906275db0fc817e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10274.47999999999, "isoelectric_point": 4.721694755554199 }, "id": "CNOCLNGIAL_302", "locus": "GCA000143945_00266" }, { "type": "cds", "sequence": "contig_1", "start": 149523, "stop": 149615, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTGGACATCTATAACAGTGCCTTTATTGTGAACTTGGACAACGTCGAATTAACGACTCCCTTACAACTGTTGCGCAATTCGACATGA", "aa": "MLLDIYNSAFIVNLDNVELTTPLQLLRNST", "aa_hexdigest": "c17b110897154ee35c51f32e14948af8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3421.9122000000007, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_303", "locus": "GCA000143945_00267" }, { "type": "cds", "sequence": "contig_1", "start": 149655, "stop": 150305, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCATTACTAAGAACGCTCAGTTAGTCATTCTAGCGGATCACACTAGTTTTGACGGTTCTAGTGTAGCTACCCTAGCCAACATCTTTGTCTCTAAACTCCAAAAGGTCAACACTGAGGGTGCCAGCGCTTTAACACCGACCATGCTTTCCTTTCCAACAGTAGACCAGGATAAGCAAAAGTATTTTAAGCAATTGAGCAAAAATTTTAAGGACTATGTGTACAATGCCGTTATGTTTGAACTGAAATGGGACTGGTTTACGAAGCCACTTATCAAAGCCAAAGGGATTAAGAACTCCGAAGCCTTTATCCATCTCTGTTACCAAATTGCCCAGTACCAAACGAACAAAAAGTTGCAGAACACCTATGTGGCCGTGGACATGCGTCAGTACTTCCGGGGACGCACCGAGTGTCTTCGTCCTTTAAGTAAACAATCAGTTGCCTTTGTCAAACGCTACTGCAAAGATCCGAAAGGTACCTTAAAGCAGTTCCGAAAGTACTACCCAGCGATTGAAAGTCTCCACTTTGAAAAAACCCGGTTAGCGCAAAAGGGTAGTGGGGTCAACCGTCACTTGCTGGGAGCATATTTGGCTTGGAATGAACACCAAGACACAATAGCTAAACCCGCTTTATTTGAAACAAAGGCTTGA", "aa": "MVITKNAQLVILADHTSFDGSSVATLANIFVSKLQKVNTEGASALTPTMLSFPTVDQDKQKYFKQLSKNFKDYVYNAVMFELKWDWFTKPLIKAKGIKNSEAFIHLCYQIAQYQTNKKLQNTYVAVDMRQYFRGRTECLRPLSKQSVAFVKRYCKDPKGTLKQFRKYYPAIESLHFEKTRLAQKGSGVNRHLLGAYLAWNEHQDTIAKPALFETKA", "aa_hexdigest": "d7166ce915cb106519eeea40626def43", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24880.50170000001, "isoelectric_point": 9.722557640075681 }, "id": "CNOCLNGIAL_304", "locus": "GCA000143945_00268" }, { "type": "cds", "sequence": "contig_1", "start": 150345, "stop": 150533, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGACAAGTATTTGCGCAACTTTTCCTTCGACCCCGTAGAGCCAAACGGCATTGGTATTGCCTATGCCATTGATGACACTAATTTCCGTGCCATTCTCAGTGTGTATCAGCACAACTTACAGTACCTCAAAGATTGGATGAAACACTTTGAACAAACAGTGAAAACAATCCTTAAAACTCTTAAATAA", "aa": "MDKYLRNFSFDPVEPNGIGIAYAIDDTNFRAILSVYQHNLQYLKDWMKHFEQTVKTILKTLK", "aa_hexdigest": "84e55af061b4a9a5d65ce1d90d3f824d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7380.4155, "isoelectric_point": 8.025427436828615 }, "id": "CNOCLNGIAL_305", "locus": "GCA000143945_00269" }, { "type": "cds", "sequence": "contig_1", "start": 150623, "stop": 151039, "strand": "+", "frame": 2, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022", "SO:0001217", "UniRef:UniRef50_P47438", "UniRef:UniRef90_P78024" ], "nt": "ATGCCGTTGATTCTTGATCAGGGCTGTTTTGTTAGTGAAAGTAGATTTCATAGATTGGCACAGAACATAAAACAAGGTTCCAGGCGTGAGCGCGAACAAAAACCGTTAATTAACGATAAAATTGGCTTTAATGAGTTCATTTTAATTGACGAAAATGGCTCCAATTTGGGGACGGTTAGAAGGACCGATGCCCTCAAGATGGCTGAGGAAAAACAACTCGATTTAGTCTTAATTGGTTCAAACCCAGCCAAACCCATTGTTAAGCTCTTAGACTTTGGTCGTTACACTTATGATTTAAAACGCAAAAAACGCCAGTCCAAGAAAAACCAAACCATTATCCAAATAAAGGAAGTGGTAGTGAAACCGACAATCGCTAAACATGATCTGGAATTTAAAGCTAAGCAAACGACGGGATGA", "aa": "MPLILDQGCFVSESRFHRLAQNIKQGSRREREQKPLINDKIGFNEFILIDENGSNLGTVRRTDALKMAEEKQLDLVLIGSNPAKPIVKLLDFGRYTYDLKRKKRQSKKNQTIIQIKEVVVKPTIAKHDLEFKAKQTTG", "aa_hexdigest": "9f687e2474f49e8dd47239253ae7c8a5", "start_type": "ATG", "rbs_motif": null, "genes": [ "infC" ], "truncated": "3-prime", "pseudogene": { "start": 150623, "stop": 151228, "observations": { "mutation": [ 151037 ], "stop": [ 151037 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78024", "query_cov": 1.0, "subject_cov": 0.6865671641791045, "identity": 1.0, "score": 267.0, "evalue": 1.59e-89, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MPLILDQGCFVSESRFHRLAQNIKQGSRREREQKPLINDKIGFNEFILIDENGSNLGTVRRTDALKMAEEKQLDLVLIGSNPAKPIVKLLDFGRYTYDLKRKKRQSKKNQTIIQIKEVVVKPTIAKHDLEFKAKQTTGWAEKGYHVKFVVRAFGRVSTRIELIEKVFNDFYLLVEPAVEVQKPLTASSKTMYSALLVPRKK" }, "paralog": false, "identity": 0.9900497512437811, "score": 389.0, "evalue": 3.76e-140, "description": "Internal stop codon at 151037. Nonsense mutation around 151037.", "psc": { "uniref90_id": "UniRef90_P78024", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_P47438", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78024", "UniRef:UniRef50_P47438", "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P47438", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47438" ], "product": "Translation initiation factor IF-3" } }, "id": "CNOCLNGIAL_306", "locus": "GCA000143945_00270" }, { "type": "cds", "sequence": "contig_1", "start": 151070, "stop": 151228, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGCGCTTTTGGTAGAGTAAGTACTAGGATTGAGTTAATTGAAAAAGTCTTTAATGACTTTTATCTGCTAGTAGAACCAGCAGTGGAGGTCCAAAAACCACTAACTGCTTCTTCCAAAACAATGTATTCTGCTTTGTTAATTCCCCGTAAGAAATAA", "aa": "MRAFGRVSTRIELIEKVFNDFYLLVEPAVEVQKPLTASSKTMYSALLIPRKK", "aa_hexdigest": "843a93a505593f211b12e21fab2f7c5d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5986.057400000001, "isoelectric_point": 9.867998313903808 }, "id": "CNOCLNGIAL_307", "locus": "GCA000143945_00271" }, { "type": "cds", "sequence": "contig_1", "start": 151234, "stop": 151413, "strand": "+", "frame": 1, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02916", "RefSeq:WP_010874473.1", "SO:0001217", "UniParc:UPI0000133FF9", "UniRef:UniRef100_P75447", "UniRef:UniRef50_P75447", "UniRef:UniRef90_P75447" ], "nt": "ATGAAAGTAAAAAGTGCTGCAAAGAAGCGTTTTAAGCTCACTAAGTCTGGCCAAATTAAGCGCAAACACGCTTATACTTCCCACTTAGCACCGCACAAAACGACGAAGCAAAAGCGCCACTTGCGCAAGCAAGGAACTGTCAGTGCGAGTGACTTTAAACGCATTGGTAATTTAATTTAA", "aa": "MKVKSAAKKRFKLTKSGQIKRKHAYTSHLAPHKTTKQKRHLRKQGTVSASDFKRIGNLI", "aa_hexdigest": "5ed192a5600e39cb80ef8f07a769af7e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FF9", "ncbi_nrp_id": "WP_010874473.1", "uniref100_id": "UniRef100_P75447", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FF9", "RefSeq:WP_010874473.1", "UniRef:UniRef100_P75447" ] }, "ips": { "uniref100_id": "UniRef100_P75447", "uniref90_id": "UniRef90_P75447" }, "psc": { "uniref90_id": "UniRef90_P75447", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P75447", "cog_id": "COG0291", "cog_category": "J", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75447" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "CNOCLNGIAL_308", "locus": "GCA000143945_00272" }, { "type": "cds", "sequence": "contig_1", "start": 151424, "stop": 151807, "strand": "+", "frame": 2, "gene": "rplT", "product": "50S ribosomal protein L20", "db_xrefs": [ "COG:COG0292", "COG:J", "GO:0000027", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904", "RefSeq:WP_010874474.1", "SO:0001217", "UniParc:UPI0000133D19", "UniRef:UniRef100_P78023", "UniRef:UniRef50_Q7NBC0", "UniRef:UniRef90_P78023" ], "nt": "ATGAGAATTAAAGGTGGAAAACAAACCCGCGTACGTCGCAAGAAGTGGTTAAAGCAAGCTAGTGGTAGCTTTGGTACCCGACATGCCTCTTATAAGGTGGCAAAGCAAACGGTGATCCAAGCCGCTAAGTATGCTTACCGCGATCGTCGTAACAAAAAGCGCGATTTCCGTTCGTTGTGGATCTTGCGCTTAAACGCCGCTTTGCGCGAACAGGGCATGACCTATTCGGTCTTTATCAATCTATTGAAGAAGCACAACATTGAGATTAACCGCAAAGTGTTGTCGGAACTAGCCATTAAAGAACCAAGTAAATTTAACCTAATTGTGCAAAAGGTTAAAAGTGAACAACCGAAAGCCGCTAAGCCAGCTGCTTTAGGAAACTAG", "aa": "MRIKGGKQTRVRRKKWLKQASGSFGTRHASYKVAKQTVIQAAKYAYRDRRNKKRDFRSLWILRLNAALREQGMTYSVFINLLKKHNIEINRKVLSELAIKEPSKFNLIVQKVKSEQPKAAKPAALGN", "aa_hexdigest": "035eea5ede4fafe36c06d492b11e94cb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D19", "ncbi_nrp_id": "WP_010874474.1", "uniref100_id": "UniRef100_P78023", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D19", "RefSeq:WP_010874474.1", "UniRef:UniRef100_P78023" ] }, "ips": { "uniref100_id": "UniRef100_P78023", "uniref90_id": "UniRef90_P78023" }, "psc": { "uniref90_id": "UniRef90_P78023", "gene": "rplT", "product": "50S ribosomal protein L20", "uniref50_id": "UniRef50_Q7NBC0", "cog_id": "COG0292", "cog_category": "J", "go_ids": [ "GO:0000027", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q7NBC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7NBC0" ], "product": "Large ribosomal subunit protein bL20" }, "genes": [ "rplT" ], "id": "CNOCLNGIAL_309", "locus": "GCA000143945_00273" }, { "type": "cds", "sequence": "contig_1", "start": 151782, "stop": 152492, "strand": "-", "frame": 1, "gene": "rnhC", "product": "Ribonuclease HIII", "db_xrefs": [ "COG:COG1039", "COG:L", "EC:3.1.26.4", "GO:0003723", "GO:0004523", "GO:0005737", "GO:0006298", "GO:0032299", "GO:0043137", "GO:0046872", "RefSeq:WP_010874475.1", "SO:0001217", "UniParc:UPI0000139442", "UniRef:UniRef100_P75446", "UniRef:UniRef50_P75446", "UniRef:UniRef90_P75446" ], "nt": "TTGCAACACGCAAACACAACCGCACTTTACCTAATTGGCAGTGATGAATCTGGCAAGGGTGATAGCTTTGGCGGAATTGCTGTAAGTGCTGTTTTAATTCATAAGGACAAAATCAATACACTCCACCAAATTGGTGTGGGGGATTCGAAGCAATTTAACGATTACCAAATTAAAGCTTTAGTGCCTAAAATCAAGGCAGCGGTTCATGACCAAGTTACATTAAGCGTTGATGCTAAAACTTACAACCAACTAGTGCAAAGCTTTAAAAATGTCAACGTAATGTTGACCTTTTTGCACTGCAAGGTGTACCACCAGTTGTTGCAACAAAACAAACTAACAGCACAACAATGTGACATTAGCATTGATGAGTTTGCTAATGTCAAACTGTTCACCCAATACACGCAAAAACTCACCAGCCTGCAAAATGAGTTAAAGGAATTAGTTATTCCCAACCACTTCTTAATTAGGGGTGAGAGCTATTCTAAGGTAATTGCCGCTGCTTCGATCTTAGCACGTGCTGCTTTTATTGCACAAATGGAACAGTTAAGCCACCAGTACGGTGTACAGTTTCCCAAGGGTAGTGCCCATGGCATTGTGGAAGCGTTACACTTACTAAAAACAAAAAGGCAGTTTCACAAGTTCGCCCAGTACAGTGCGGTCTGTAAAACTACCTTTAAGAATGTCGCTAGTTTCCTAAAGCAGCTGGCTTAG", "aa": "MQHANTTALYLIGSDESGKGDSFGGIAVSAVLIHKDKINTLHQIGVGDSKQFNDYQIKALVPKIKAAVHDQVTLSVDAKTYNQLVQSFKNVNVMLTFLHCKVYHQLLQQNKLTAQQCDISIDEFANVKLFTQYTQKLTSLQNELKELVIPNHFLIRGESYSKVIAAASILARAAFIAQMEQLSHQYGVQFPKGSAHGIVEALHLLKTKRQFHKFAQYSAVCKTTFKNVASFLKQLA", "aa_hexdigest": "a21daf5ea682856f414fb271068b4c4c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139442", "ncbi_nrp_id": "WP_010874475.1", "uniref100_id": "UniRef100_P75446", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139442", "RefSeq:WP_010874475.1", "UniRef:UniRef100_P75446" ] }, "ips": { "uniref100_id": "UniRef100_P75446", "uniref90_id": "UniRef90_P75446" }, "psc": { "uniref90_id": "UniRef90_P75446", "gene": "rnhC", "product": "Ribonuclease HIII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_P75446", "cog_id": "COG1039", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0032299", "GO:0046872", "GO:0003723", "GO:0004523", "GO:0043137", "GO:0006298" ] }, "pscc": { "uniref50_id": "UniRef50_P75446", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75446" ], "product": "Ribonuclease HIII" }, "genes": [ "rnhC" ], "id": "CNOCLNGIAL_310", "locus": "GCA000143945_00274" }, { "type": "cds", "sequence": "contig_1", "start": 152548, "stop": 153273, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00226.36" ], "nt": "ATGGCAGAGGCAAAACGTGATTATTATGAAGTCTTAGGGCTTAGTCGGGATGCTGATGATAACGACATAAAAAAGGCCTTTCGTAAACTAGCCAAAAAGTACCACCCCGACCGCAATAAGGCACCGGATGCGGCTCAAATTTTTGCCGAAATTAACGAGGCCAATGACGTGTTGTCTAACCCCAAAAAACGTGCTAACTATGACAAATACGGCCATGATGGGGTGGACAATGAAGGTGGTTTTGCCTTTCAAGCTGATGTGTTTGATTCTTTTTTTGAGGAGATTGAAAAATCGGGTGCCTTTGATAATTTAAGTGAGTCTAACACCAAAAAGAAAGAGAAGACTAAAACTAAGAAGAAGGGTTGGTTTTGGGGCAAATCCAAACAGGAAGAATCTACCAGCGATACTACTGAATATGCCGATGTTGACGCTGGTTTAGAAGATTATCCGCCTCAATCTGATTATCCAGATGATATTCCTGATGTGGATGCCAGGATCGAAGAAGTGGATCAATCTGCCTATGCCGATGACATTCCCGATGTAGATGCGGGAATGGATTGGGAACAAAACGCAGAAGTAGCAAATTCTGCTAGTGAAATCATCCCCGATGTCGATGCTGGTTTAGCGGATGAGTTTAATACTAGTAGTGCAGCACCCCAAGCTAGTGACTGGGAAGCAATGATTGGTAATCCTGAGTACGGTTACTTTGATGCTGCTGGTGAGTGA", "aa": "MAEAKRDYYEVLGLSRDADDNDIKKAFRKLAKKYHPDRNKAPDAAQIFAEINEANDVLSNPKKRANYDKYGHDGVDNEGGFAFQADVFDSFFEEIEKSGAFDNLSESNTKKKEKTKTKKKGWFWGKSKQEESTSDTTEYADVDAGLEDYPPQSDYPDDIPDVDARIEEVDQSAYADDIPDVDAGMDWEQNAEVANSASEIIPDVDAGLADEFNTSSAAPQASDWEAMIGNPEYGYFDAAGE", "aa_hexdigest": "b22a96e26c130cdba3c187414383c4f4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00226.36", "name": "DnaJ", "length": 63, "aa_cov": 0.2572614107883817, "hmm_cov": 1.0, "evalue": 1.3564213863448636e-27, "score": 91.14558410644531, "start": 7, "stop": 68 } ], "seq_stats": { "molecular_weight": 26822.45639999997, "isoelectric_point": 4.249249076843263 }, "id": "CNOCLNGIAL_311", "locus": "GCA000143945_00275" }, { "type": "cds", "sequence": "contig_1", "start": 153469, "stop": 154221, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACCGGAAACTGAACCAGAACCAATTATTACCTTAAGTTCAGAGCCAGTGGAAGCACCAGCTTCTGTTGTGATTGAACCTACCCCTGAAACTGAGGAAACAACTAGTGCTGTAGAAATGGATGCTTCAGTTAAAGCAGATGTTTCTGATGAAGCTGACGCAACTAATGAACCAACTGAACAGGATACCATTTCAGAGCCAGAACAAGAAACAGACGCGGCTGCATTAGAAGAAATTAACCACACCACTGCTGATTTGGAACCAGCAGAAGTTAGTGCTACTAATGATCTAGAACAAGATGTAGTAGAGAAAGTTAATTTTTCAGAACCAGAATCAACAGTAGATACTGCTGCAACCGATCCTGTTGTAGAACAAGCTACGGAAACCTCCACCAATGGTTTCAAATTCTTTAACTTTAGTTCGTTTGTTTTAAGTGACCAAAACCCCAATCCACAAACACCCACCCACCATGAAGAAGATGCTGCAGCCCCCGAGCCAACCGTTGATGAAACTTCTGGAGAATCCACAGCCCCCGAAGTTACCATAGCTGAGTCAACTGTAGAATTGGAAACCGCAGCGGAAATTAATAACCCCGCTACCTTTGTGGAAGAATACCTCCAACCAACTAAAACTACGGTTGTTGATAAATTAGATGAACCAACTGTAGCAAAACCTACTGTTAGCGATTCTGAAAATTCAGTTGCACCAGAACCGGAATTTGTTGCTGGACCGGAACAAACCTTTAGCTGA", "aa": "MQPETEPEPIITLSSEPVEAPASVVIEPTPETEETTSAVEMDASVKADVSDEADATNEPTEQDTISEPEQETDAAALEEINHTTADLEPAEVSATNDLEQDVVEKVNFSEPESTVDTAATDPVVEQATETSTNGFKFFNFSSFVLSDQNPNPQTPTHHEEDAAAPEPTVDETSGESTAPEVTIAESTVELETAAEINNPATFVEEYLQPTKTTVVDKLDEPTVAKPTVSDSENSVAPEPEFVAGPEQTFS", "aa_hexdigest": "99b832c60caa1eb0ae0d7749e70ec51e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26726.076700000005, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_312", "locus": "GCA000143945_00276" }, { "type": "cds", "sequence": "contig_1", "start": 154669, "stop": 155280, "strand": "+", "frame": 1, "gene": "dnaJ", "product": "DnaJ C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557D12" ], "nt": "TTGAACGAACAGCTCAAGGAAATTAGGTACACCCGTAAGTTAGTCGATCCACAAACTCAGGTAACCACCACTGAATCAATTACCCTAGAAGTTCAGCTATCACATAAATCCCAAACGGAAGCAATTGCCATCTTTAAGGGCTTTGGTCATGACTATGGTAGTGGCTGTGGTGACCTTAAGGTAGTACTTAAAGTGATCCCTAGTGTCTTTTTCCAACTGCAAGCTGATGGCTTACACACCGCTGCCCTAGTCGACCCTCTAGTGGCTTACAACGGTGGTTTAATTGATTTCTTTGGTCCAGTTAACAGCTTTAAAGTTGATATTGAAGGTGGGATTGCTAACAACGATTTAATTGAGTTTAACCAGTTAGGAGTACTAAGAACTAAGACCAAACGTGGTAGTTTATACGTCCACTTGTACTATAGTAGTGTAGCCAAAAAGGGCACTAAGACTAACTGCCAAGTGCAACAGTTCTTTGATCTAGTTCATGTGGAATACAAGCTGTTAAACTACAACTTAAAACAACTCCACAACTACCACTCGGCTCTCACTGCCCAGAAAAAAACCTTGGACCGCAAAAGCTATCAATGTTTAGCAGTGGAAAGCCATTAA", "aa": "MNEQLKEIRYTRKLVDPQTQVTTTESITLEVQLSHKSQTEAIAIFKGFGHDYGSGCGDLKVVLKVIPSVFFQLQADGLHTAALVDPLVAYNGGLIDFFGPVNSFKVDIEGGIANNDLIEFNQLGVLRTKTKRGSLYVHLYYSSVAKKGTKTNCQVQQFFDLVHVEYKLLNYNLKQLHNYHSALTAQKKTLDRKSYQCLAVESH", "aa_hexdigest": "9c19cea1e031089383b878fc031ac15a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557D12", "query_cov": 0.9014778325123153, "subject_cov": 0.9838709677419355, "identity": 0.536, "score": 193.0, "evalue": 1.92e-59, "valid": false, "product": "DnaJ C-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0000557D12" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557D12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557D12" ], "product": "DnaJ C-terminal domain-containing protein" }, "genes": [ "dnaJ" ], "id": "CNOCLNGIAL_313", "locus": "GCA000143945_00277" }, { "type": "cds", "sequence": "contig_1", "start": 155303, "stop": 155755, "strand": "+", "frame": 2, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "GO:0000774", "GO:0005737", "GO:0006457", "GO:0042803", "GO:0051082", "GO:0051087", "KEGG:K03687", "SO:0001217", "UniRef:UniRef50_P47443", "UniRef:UniRef90_P78017" ], "nt": "ATGAGCGAAAACTCACTCACAATTACCGAAATTCTCAGTTCCATCCGTACGCTTTTAGTCAAACACAATAAGGCTAAAGTAACCCAAATTGAAAAGGAACTTTTACAAGCAGTTGCTGAACTGGAAAAGAAGTTTAAGCAACAAGTACAAAACTTCAACGAGTTGCAACAAAAGATTCCTAACTTACAAAAAGTCAACGAGGAATTTCGCTTAAAAGTGGAAAAAATCCAGGAAGAAGCGCAGAAGAAAATTCAGGAAAAAGTGGCAGAACTGACCATTAAAAGTAAGGAAGAGTTGGAAAATGCAAAGAAGTATGTGATCGAAAAGTCGATTGATCAACCCTTAATTATTATTGACCAGTTCGAAATTGCACTTTCCTATGCTCAAAAGGATCCCCAAGTAAAAAACTATACCACTGGGTTTAACATGGTGTTGGATGCCTTTTCGCGCTGA", "aa": "MSENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIPNLQKVNEEFRLKVEKIQEEAQKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQFEIALSYAQKDPQVKNYTTGFNMVLDAFSR", "aa_hexdigest": "b4cb6dcd106da9db7a74149b5bbc8cc3", "start_type": "ATG", "rbs_motif": null, "genes": [ "grpE" ], "truncated": "3-prime", "pseudogene": { "start": 155303, "stop": 155956, "observations": { "mutation": [ 155753 ], "stop": [ 155753 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78017", "query_cov": 1.0, "subject_cov": 0.6912442396313364, "identity": 1.0, "score": 266.0, "evalue": 6.31e-89, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MSENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIPNLQKVNEEFRLKVEKIQEEAQKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQFEIALSYAQKDPQVKNYTTGFNMVLDAFSRWLEGFGVTKIAIEPGAQFDEKVMAALEVVPSDQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE" }, "paralog": false, "identity": 0.9907834101382489, "score": 407.0, "evalue": 7.4e-147, "description": "Internal stop codon at 155753. Nonsense mutation around 155753.", "psc": { "uniref90_id": "UniRef90_P78017", "gene": "grpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_P47443", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687", "go_ids": [ "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78017", "UniRef:UniRef50_P47443", "COG:COG0576", "COG:O", "KEGG:K03687", "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ] }, "pscc": { "uniref50_id": "UniRef50_P47443", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47443" ], "product": "Protein GrpE" } }, "id": "CNOCLNGIAL_314", "locus": "GCA000143945_00278" }, { "type": "cds", "sequence": "contig_1", "start": 155819, "stop": 155956, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCACTGGAAGCTGTGCCTTCTGATCAACCGGCTAATACGGTGGTGAAGGTATCGAAATCGGGTTACAAACTCCACGACAAGGTCATTCGCTTTGCTTCCGTAGTGGTAAGCCAAGGTAATAAAACAGAATAA", "aa": "MAALEAVPSDQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE", "aa_hexdigest": "30c7f6f32836c5f5415e21333296a8de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4800.448800000002, "isoelectric_point": 9.302868461608885 }, "id": "CNOCLNGIAL_315", "locus": "GCA000143945_00279" }, { "type": "cds", "sequence": "contig_1", "start": 155956, "stop": 156321, "strand": "+", "frame": 1, "gene": null, "product": "MPN121 family protein", "db_xrefs": [ "RefSeq:WP_010874478.1", "SO:0001217", "UniParc:UPI0000139461", "UniRef:UniRef100_P75353", "UniRef:UniRef50_P75353", "UniRef:UniRef90_P75353" ], "nt": "ATGAGCACAAATAAAAGAAGAACCATTCAGATTGAAATCACTGAAGAACACTTCAAGGATTTAGAAAAAGCCTTGGAAGCCTTGAAGGGTACCCAATTACCCTTCTCCACTACCGTAGAACAGTTTGTGGAATTAATTTTGTCCAACTATGTCGCTACTTCCAACAAGATTTCTAATTTAGCTGAAAGTGGCTTTGATGTGGCTTCTATTCAACAAGAATTAGAAAAAATTGGCAGCGCTGCAGGGGCGGATGATGCGCTCAAAAGCTTCTTGGATGAGTTGTTAAAAACATCGCAAAAAAGCTTTAGCAATACCAAAGATGGCAAAAAAAATGATGACGATAATAATTCGTCATCAAAATCATAA", "aa": "MSTNKRRTIQIEITEEHFKDLEKALEALKGTQLPFSTTVEQFVELILSNYVATSNKISNLAESGFDVASIQQELEKIGSAAGADDALKSFLDELLKTSQKSFSNTKDGKKNDDDNNSSSKS", "aa_hexdigest": "58ad45def04906f3e98d12365a0a2c20", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139461", "ncbi_nrp_id": "WP_010874478.1", "uniref100_id": "UniRef100_P75353", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139461", "RefSeq:WP_010874478.1", "UniRef:UniRef100_P75353" ] }, "ips": { "uniref100_id": "UniRef100_P75353", "uniref90_id": "UniRef90_P75353" }, "psc": { "uniref90_id": "UniRef90_P75353", "product": "MPN121 family protein", "uniref50_id": "UniRef50_P75353" }, "pscc": { "uniref50_id": "UniRef50_P75353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75353" ], "product": "Uncharacterized protein MG202 homolog" }, "genes": [], "id": "CNOCLNGIAL_316", "locus": "GCA000143945_00280" }, { "type": "tRNA", "sequence": "contig_1", "start": 156339, "stop": 156412, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 62.8, "nt": "GCCGAATTAGTTTAGGGGCAAAACAGATCCATGGTAAGGATCAGAGAACAGTTCGACTCTGTTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 156378, 156380 ], "id": "CNOCLNGIAL_33", "locus": "GCA000143945_00281" }, { "type": "cds", "sequence": "contig_1", "start": 156447, "stop": 158102, "strand": "+", "frame": 3, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47445", "UniRef:UniRef90_P78016" ], "nt": "ATGAAAAATAATTACAGCGAAGCGAATATCAAAATTCTCAAGGGGCTAGATGCTGTAAAAAAACGCCCGGGAATGTACATCGGCTCAACCGATAGTCGCGGTTTTCACCACTTGCTGTGGGAAATTTTAGACAACTGTGTCGATGAAGTTTTATCGGGCTTTGCTAATACTATTGCCGTTGTTTTACATGCCGAAAACCAGATAACTGTTAGTGACAACGGTCGTGGTATTCCCTTCGAAACACACTCCGATTCCAAGATTTCTACGATTGACACCGTCTTTACCTACCTCCATGCCGGTGGTAAGTTTGATAACGATTCTTATAAGATTGCTGGAGGGTTGCACGGGGTGGGGGCTTCGGTGGTGAATGCCTTGAGTGACCAGTTACAAGTAACGGTTAAACGTCAGGGCAAGGTGTACCGCTCGGTGTATGAAAATGGTGGTAAGATTAAGCAAAAAGCGCATTGCATTGGTAATGCCAAAATTGACGAACACGGTACCAGTGTGACTTTTCGTCCTGACCCAAAGGTCTTTAAAAAGATCCACTTTGACAGCGAACTAATTCGTGCGCGGTTAAAGGAATTAGCCTTCCTTTTCAAAAAGTTACAACTGACCTTCGTTGATGAAACCGGCAGTGGGGAAAAAGAGGTGTTCTTTACCGAAGCCGGTATTAGTCAGTACTTGGACGAACTTAACGCTGATTCCAAACAAATTGCGCAAAAGATCTTTGTTAGTGGCACTGAAGATGACATTGAACTGGAAGCCGTGTTTCAGTTTATTGATGGGGAGGATGAAAAACTACTTTCGTTTGCCAATTCGATTCGCACCAGCGAAGGGGGTAGTCATGAAGCGGGTTTTCGTCAATCTGTTGGTGATGTGATTAACAACTACTGCCGCAAGTACAACTTCCTAAAGGAGCGTGACAAGAACTTTGAGGCCAGTGAAATCCGTGAAGGCTTAAACGGCATTATTAAGGTCAATCTCCCCGAAAAGATTATTGCCTTTGAAGGGCAAACGAAGAGCAAACTGTTCTCCAAGGAAGTTAAAGCGGTAGTGCAAAAGCTAACCCAAAAGCACTTCTTCCAGTTCTTGGAACGTAACAGTGTTGATGCTAAATTAATTGTCGAAAAGCTGTTTTATAACCGCAAACTGCGCCAAGAACTCAAACAACAACGCCAAATTAAAAAGAACCTTTCTAATCCGAAGGCCGAGCGCATTTTGTTTGGTAAATTAGCACCGGCGCAGTCCAAAAAAGTGGCTGAACGCGAACTGTTTGTGGTGGAAGGTGACAGTGCTGGTGGTACGGCTAAAATGGGACGAGATCGCTTTCTACAAGCCATCTTACCGTTACGCGGTAAGGTGTTAAATGTGGAGAAAATTAACAACAAGAAGGAAGCGATTAACAACGAAGAGCTGTTAACACTCATCTTCTGTATCGGTACTGGTATTGGTAACAACTTCACCATTCGCGATCGTAAATACGACAAGATTATTATCATGACTGATGCTGACAATGACGGTGCGCACATCCAGATTTTACTGCTCACCTTCTTCTACCGTTACATGAAACCCCTAATTGAAAAAGGACACATTTACCTAGCACTTCCACCACTTTATAAGTTTGAAGGTCGTGATAAAAAAGCCCGTTATCTCTGA", "aa": "MKNNYSEANIKILKGLDAVKKRPGMYIGSTDSRGFHHLLWEILDNCVDEVLSGFANTIAVVLHAENQITVSDNGRGIPFETHSDSKISTIDTVFTYLHAGGKFDNDSYKIAGGLHGVGASVVNALSDQLQVTVKRQGKVYRSVYENGGKIKQKAHCIGNAKIDEHGTSVTFRPDPKVFKKIHFDSELIRARLKELAFLFKKLQLTFVDETGSGEKEVFFTEAGISQYLDELNADSKQIAQKIFVSGTEDDIELEAVFQFIDGEDEKLLSFANSIRTSEGGSHEAGFRQSVGDVINNYCRKYNFLKERDKNFEASEIREGLNGIIKVNLPEKIIAFEGQTKSKLFSKEVKAVVQKLTQKHFFQFLERNSVDAKLIVEKLFYNRKLRQELKQQRQIKKNLSNPKAERILFGKLAPAQSKKVAERELFVVEGDSAGGTAKMGRDRFLQAILPLRGKVLNVEKINNKKEAINNEELLTLIFCIGTGIGNNFTIRDRKYDKIIIMTDADNDGAHIQILLLTFFYRYMKPLIEKGHIYLALPPLYKFEGRDKKARYL", "aa_hexdigest": "31e29ceb8f1bad27701229cbeb646df2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P78016", "query_cov": 1.0, "subject_cov": 0.8677165354330708, "identity": 0.998, "score": 1073.0, "evalue": 0.0, "valid": true, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47445", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47445", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47445" ], "product": "DNA topoisomerase 4 subunit B" }, "genes": [ "parE" ], "id": "CNOCLNGIAL_317", "locus": "GCA000143945_00282" }, { "type": "cds", "sequence": "contig_1", "start": 158115, "stop": 158354, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_T0TWZ4" ], "nt": "TTGGAACAGTACCGTGCTAAGCATAGCCACTTTAATGTGCAACGGTACAAGGGTTTAGGGGAAATGAATGCTGATCAGCTCTGGGAAACCACGATGAACCCGATGACGCGTAAACTAATTCAGATCAAATTGGATAACTTTATCCAAGCTGAAAAGCAAATTAATGTTTTTATGGGTGATAAAACCGAATTGCGTAAAAGCTGGATTGAAGCTAACATTAACTTTAGTAGTGAGAACTAA", "aa": "MEQYRAKHSHFNVQRYKGLGEMNADQLWETTMNPMTRKLIQIKLDNFIQAEKQINVFMGDKTELRKSWIEANINFSSEN", "aa_hexdigest": "ca5d3a6c14265065c0ae25069908898a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1Q6V0H2", "query_cov": 0.8607594936708861, "subject_cov": 0.8395061728395061, "identity": 0.603, "score": 91.7, "evalue": 9.51e-23, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_T0TWZ4" }, "pscc": { "uniref50_id": "UniRef50_T0TWZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_T0TWZ4" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "CNOCLNGIAL_318", "locus": "GCA000143945_00283" }, { "type": "cds", "sequence": "contig_1", "start": 158354, "stop": 158641, "strand": "+", "frame": 2, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "nt": "ATGGAAAAGAATAAACAAGCGTTATTACTCCAGGCCATCGAAGATGTCTTTGCCTTTAGCTTTAGTAAGTATGCTAAGTACATTATCCAAGACCGGGCTTTACCTGATCTACGTGATGGGCTTAAACCAGTTCAACGCCGCATCCTCTATGGCATGTACCAAATGGGCTTAAAACCCACTTCACCCTACAAAAAGTCAGCTCGGGCAGTCGGAGAAATTATGGGTAAGTACCACCCCCATGGCGATGCATCGATCTATGATGCGATTGTAAGGATGTCACAAGCCTGA", "aa": "MEKNKQALLLQAIEDVFAFSFSKYAKYIIQDRALPDLRDGLKPVQRRILYGMYQMGLKPTSPYKKSARAVGEIMGKYHPHGDASIYDAIVRMSQA", "aa_hexdigest": "5a748c3ede98040626375442c755abc5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028540D82", "query_cov": 0.8736842105263158, "subject_cov": 0.83, "identity": 0.6629999999999999, "score": 124.0, "evalue": 3.5e-35, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_Q9XCU2" }, "pscc": { "uniref50_id": "UniRef50_Q9XCU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "CNOCLNGIAL_319", "locus": "GCA000143945_00284" }, { "type": "cds", "sequence": "contig_1", "start": 158711, "stop": 160723, "strand": "+", "frame": 2, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335", "SO:0001217", "UniRef:UniRef50_P47446", "UniRef:UniRef90_P75352" ], "nt": "ATGCGCTATACGGAAGCGCGTTTAAGTCCGTACGGGTTTGAACTACTCAAAGACATTGAAAAGCAGTTAGTACCGTTTGTGAATAACTTTGATGACAGTGAGGTCGAACCGAGCGTGTTACCAACCCTGTTACCTAACCTCTTTATTAACGGTACCAGTGGAATAGCGGCGGGTTATGCCACTAATATTGCACCCCATAATGTCGGTGAACTGCTTGATGGTTTGAGTTACCGGATTGAAAACCCTGATTGTGATTTAAAGGCCATCTTAAAGATTGTTAAGGGACCGGATTTTCCAACTGGTGGATTAGTGTACTTTGAGCAAGAGTTGGCGAACATCTACCAAACTGGGAAGGGTAAGTTTGTGATTCAGGCCAAATATGAAACGAACACTGCGTTTGGCCAAAACCAGATTGTAATCACCGAAATCCCATACGAAACGGTGAAGGCCAATATTGTTAAACAAATTGAAGAGTTAATTAGTGACAATAAACTCAGTGCCTTGGAAAGTGTGATTGACAGCTCTGATCGTAGTGGTATTCGGATCATTATTAACCACAAGGACTTTTTGAGTGCTGACAAGATTATGGCGTTCTTGTTCAAACACACCCAACTCCAAGTAAACTTTAACCTCAACAATACGGTAATTGCCAACCGTTGTCCGGTACGGGTGGGGTTGTTAGCTTACTTTGATCAGTTCCTAGCCTTTGCCCACGAATTGATCATTAACAGCGCCAAGTATGATTTAGCATTGGCCAATAAGCGCTTGGAAATCATTAAGGGCTTAATTAAGGCGGTTTCGATGATTGATGAAATTATTCGTTTAATTCGCCGTGCTACTGACAAACAGGATGCGAAAACCAAGCTCATTGACAAGTATGCTTTTACCCTAAATCAAGCCGAAGCGATTGTGTCGTTGCGTTTGTACCAGTTAACTAACACCGACATTAAGGTCCTGTTTGCCGAACAAAAAGAGTTAGAGCAAACGATCCAAACAGCCGAATGCTTAATTGCTAAACCGCAAGCACGAAACCAGTTGTTACAAGCACAGTTTTTCCAATACAAAAAGCAGTTTAACCAACCACGACGCGCACAAATAGTTGGTTTAATTGAAAAGCAAAAGGTACAAGACAGTGACTTCATCGAACACAAGGAAGTGGGCTTACTCATTAGTCACGATGGCATCTACTTTAAGTTTGAACCAGAACAGTTAGCGAAGCACCTAGTGGAATTTAAAAGTGAACAAGACCAACTGATTTTTGGAGGCGTAGTACAAAACTCCGATTACTTTTTTATGGTGACTTCACTCGGTAACATTATTACCGTTCCCATTTACAAAACGTTAAGTAACACCAAAACCAAGATGAACGAGTTGTTGGCCAAAAAACCGATCCTAATGGAAGATGAAAAATTGGTACTAGCAGGGATTGTAAACCCAGACAAAATGGAACAACAGCTCTTGGTGTTAACGTCACAGTGCGGTATGGTCAAACGGGTCGAGTTGAGCAAAGTCATTAACACGAAGCAAATTAAGTCAAGCTGCTGCATGGCACTCCGGGAACGCGACAAACTGGTCAACGCCTTTGTTCAAACTAAAAGGGAACCTAAACTAGTTTGTCTGGTGTCATCTTCCAACAGTTTTGCTACCTTCTTAGCGGAAGAAATTCCAATTATTAGTAACAAGGGAATTGGGGTGAAGGGGATTAAACTCAAAGCTGAGGAAAAGGTACGGTTTGCAATGCCGTTACAGGATAATGATGCCTTAGTAGTGATTAACAGTGATGGTGGTGTCTATAACTTTGAAGTAGTGGAACTGGCAGTCGCTAGCCGCATGAGTGTTGGTAAAAAACTAATTCCCAAAACCAAAACCCCGGTAAGCTGTTTTGCTGCTAATAAGCACAGTGAGATTATTGGTCATCGTGGTAAAAATGGTAGTGATCTGTTTACCCTAAATGAGTTAAACCGTTTACCAAAGTCAACCGTTAGTCAAATGCGTTTGTTTAAGTGGAGCTAG", "aa": "MRYTEARLSPYGFELLKDIEKQLVPFVNNFDDSEVEPSVLPTLLPNLFINGTSGIAAGYATNIAPHNVGELLDGLSYRIENPDCDLKAILKIVKGPDFPTGGLVYFEQELANIYQTGKGKFVIQAKYETNTAFGQNQIVITEIPYETVKANIVKQIEELISDNKLSALESVIDSSDRSGIRIIINHKDFLSADKIMAFLFKHTQLQVNFNLNNTVIANRCPVRVGLLAYFDQFLAFAHELIINSAKYDLALANKRLEIIKGLIKAVSMIDEIIRLIRRATDKQDAKTKLIDKYAFTLNQAEAIVSLRLYQLTNTDIKVLFAEQKELEQTIQTAECLIAKPQARNQLLQAQFFQYKKQFNQPRRAQIVGLIEKQKVQDSDFIEHKEVGLLISHDGIYFKFEPEQLAKHLVEFKSEQDQLIFGGVVQNSDYFFMVTSLGNIITVPIYKTLSNTKTKMNELLAKKPILMEDEKLVLAGIVNPDKMEQQLLVLTSQCGMVKRVELSKVINTKQIKSSCCMALRERDKLVNAFVQTKREPKLVCLVSSSNSFATFLAEEIPIISNKGIGVKGIKLKAEEKVRFAMPLQDNDALVVINSDGGVYNFEVVELAVASRMSVGKKLIPKTKTPVSCFAANKHSEIIGHRGKNGSDLFTLNELNRLPKSTVSQMRLFKWS", "aa_hexdigest": "c0e9c81703a425db3fd07f8fe110609c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75352", "query_cov": 1.0, "subject_cov": 0.8491761723700887, "identity": 0.997, "score": 1283.0, "evalue": 0.0, "valid": true, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47446", "cog_id": "COG0188", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47446", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47446" ], "product": "DNA topoisomerase 4 subunit A" }, "genes": [ "parC" ], "id": "CNOCLNGIAL_320", "locus": "GCA000143945_00285" }, { "type": "cds", "sequence": "contig_1", "start": 160710, "stop": 160961, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTTTATGAACCAAAACAACCAGACTACTAATATTACGTTTGGTGACAAGTTAGGATTAGAAGGGGAAGAGGTTTCGGTAGCTTCTACTCTTATTAATACCACCAACGAAAGTAAGCACCAACTAGCGATAGTGGGTCCCACCCGGATGGACTACCAAAAGGTGAAAGCGTTATTGTTGACCCTCAAAGAAGAGATCGAGGAGTACGATAAGCAACTTCACGGTGGTAAAACCACTAGCTCCACTTAA", "aa": "MAFMNQNNQTTNITFGDKLGLEGEEVSVASTLINTTNESKHQLAIVGPTRMDYQKVKALLLTLKEEIEEYDKQLHGGKTTSST", "aa_hexdigest": "6158fecdc70c3a4303ea66eef2e7d5af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9202.240299999998, "isoelectric_point": 5.237963294982909 }, "id": "CNOCLNGIAL_321", "locus": "GCA000143945_00286" }, { "type": "cds", "sequence": "contig_1", "start": 160968, "stop": 161765, "strand": "-", "frame": 1, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "GO:0045892", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_P75351", "UniRef:UniRef90_P75351" ], "nt": "ATGAAAAATTTAACTACCCGACAAGCCCAAATTCTCAAGGCCATCATCAACGAATATATTGCCTATCCAGTACCTGTTGGTAGTAAATTATTAACTAAAAAATATTTTAAAAACCTCTCTGGCGGTACACTCCGAAACGAAATGGCCGTTTTGGAAAAAGAGGGTTATCTCAAAAAGAACCACATCTCTTCAGGACGAATCCCCTCCCAGTTGGGCTATCAGTACTATGTCAAGTTACTCACTAAAAATGATGATAAGAGTAACCTTAAAACCCGCTTAAGGGCGATTATCTTGCAAAAACACAAAACAATTGATGAAATTATTGAACTGGGGGTAAAGTTTATCAACGAAATGGTAAACCTACCAGTGGTGTTAACCCACTTTAGTAGTGATGAAGTCTTAAAAAAGATTGATCTCATTATGCTCGATCAGTCCTGTGCACTGCTACTGTTAGTGTCAGCTAGTGGTAACGTCTTTAAGAAAACGATTAGCTATGCTAACCAAAGACAGTTTGAAGACATCATGGTATGTGTCAGGATCTTTAATGATCGCATTATCGATACACGTTTCTGTGATATTGCCCAACACTTAGATGTTTTAAAGGAAATTATTCGCAGCAAGGTACACGAGTACCAGTATGTCATTGACGAAATCCTGTTTAAGCTGTTTAACTTTGAGGAATTCCAACAAGCACGTAAGCAGGTTTATGGCATCCATTACCTAGCCCAACAACCAGAGTTTGCCAACCAGGAAAGATTGACGCGTATCTTAAACTTACTGGAAGATACTAGTGTCTGA", "aa": "MKNLTTRQAQILKAIINEYIAYPVPVGSKLLTKKYFKNLSGGTLRNEMAVLEKEGYLKKNHISSGRIPSQLGYQYYVKLLTKNDDKSNLKTRLRAIILQKHKTIDEIIELGVKFINEMVNLPVVLTHFSSDEVLKKIDLIMLDQSCALLLLVSASGNVFKKTISYANQRQFEDIMVCVRIFNDRIIDTRFCDIAQHLDVLKEIIRSKVHEYQYVIDEILFKLFNFEEFQQARKQVYGIHYLAQQPEFANQERLTRILNLLEDTSV", "aa_hexdigest": "53d5a70a4a46a8ca087e08370db515f7", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 160710, "stop": 161765, "observations": { "mutation": [ 160970 ], "stop": [ 160970 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75351", "query_cov": 1.0, "subject_cov": 0.7549857549857549, "identity": 1.0, "score": 510.0, "evalue": 2.98e-181, "gene_start": 1, "gene_end": 265, "reference_start": 1, "reference_end": 265, "reference_sequence": "MKNLTTRQAQILKAIINEYIAYPVPVGSKLLTKKYFKNLSGGTLRNEMAVLEKEGYLKKNHISSGRIPSQLGYQYYVKLLTKNDDKSNLKTRLRAIILQKHKTIDEIIELGVKFINEMVNLPVVLTHFSSDEVLKKIDLIMLDQSCALLLLVSASGNVFKKTISYANQRQFEDIMVCVRIFNDRIIDTRFCDIAQHLDVLKEIIRSKVHEYQYVIDEILFKLFNFEEFQQARKQVYGIHYLAQQPEFANQERLTRILNLLEDTSVWQQMAFMNQNNQTTNITFGDKLGLEGEEVSVASTLINTTNESKHQLAIVGPTRMDYQKVKALLLTLKEEIEEYDKQLHGGKTTSST" }, "paralog": false, "identity": 0.9971509971509972, "score": 682.0, "evalue": 3.47e-251, "description": "Internal stop codon at 160970. Nonsense mutation around 160970.", "psc": { "uniref90_id": "UniRef90_P75351", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_P75351", "cog_id": "COG1420", "cog_category": "K", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355", "GO:0045892" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75351", "UniRef:UniRef50_P75351", "COG:COG1420", "COG:K", "KEGG:K03705", "GO:0003677", "GO:0006355", "GO:0045892" ] }, "pscc": { "uniref50_id": "UniRef50_P75351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75351" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "CNOCLNGIAL_322", "locus": "GCA000143945_00287" }, { "type": "cds", "sequence": "contig_1", "start": 161890, "stop": 162879, "strand": "+", "frame": 1, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_P47448", "UniRef:UniRef90_P75350" ], "nt": "TTGTATGTCGGGAAGGCTAAGGACATCTTTAAGCGGGTACACCATTACTTTAATCCAAACCGTTCCTTTAAAACACGGGCACTAGTGGAGCGAATTGCGGATGTCGAATACGTCATTTTGAAAAATGAAAATGATGCTTTGAACTTAGAGGCCAAACTAATTAAACAGTACAAACCGCGCTTTAATTTAGTTTTAAAGGAAAATAATGGTTATCTCTATTTCTTTATTACCGCCAGTGTTAAACCCACCTTGGAATTGGGCCGCCGGTATGAGTTTTCCAAGAATAAGTACTTCGGTCCGTTTGCATCGAGTAAGTTTCGACTGCGTGACATTTATGACCTGTTGTTAAAGCTTTTTCCATTACGCAAGTGCGCGCCCCATGAACGGGGTCACCCCTGCTTTTACTATCAGCTTAAAATGTGCATGGGTCAGTGTATGGGCGAGGATACACCGGAACGTTACCAGACCACCGTCAAGGGAATTGAACAGTTTTTTAACCACGGTCCCGAGCAGGTTTTAAACCACTTGCAACAGCAAGAAATCAAGGCTAGTGAACAACAAAACTTTGAAGCAGCACGCCACTTCTTGGACTTACAGAAAGCCGTTTTAGAACTAGTGAACATGCAACAAACGGCCTTCATTAAAGCAAAGCAAAGCCATGACTTTATTGGTTATGTCTTTGAGAAAAATGTGTTAGCCATTACCGTCTTTGCTTACGTTGATAACCAACTAATAGGCAAAAACCAGCAGGTCTTTGAACTACCACAGGACGATGAAAAGGAAGTTGAAAGTGCTTTGGTTACCTTTATTTACCACTATTATTCCACCAATAAGATACCCAAAACCCTTACAGTTTCTTTAAGCGAAGAGAATTTGAGCTTACTGGCCAACAGTCTTAAAATTAATGTTACGCAACCAAAAAACGGCGAGCAAAAATCCATTTTGCAAACCGTGATTGACAACGCCCGTTATGCCTTAAACACCAAGTGA", "aa": "MYVGKAKDIFKRVHHYFNPNRSFKTRALVERIADVEYVILKNENDALNLEAKLIKQYKPRFNLVLKENNGYLYFFITASVKPTLELGRRYEFSKNKYFGPFASSKFRLRDIYDLLLKLFPLRKCAPHERGHPCFYYQLKMCMGQCMGEDTPERYQTTVKGIEQFFNHGPEQVLNHLQQQEIKASEQQNFEAARHFLDLQKAVLELVNMQQTAFIKAKQSHDFIGYVFEKNVLAITVFAYVDNQLIGKNQQVFELPQDDEKEVESALVTFIYHYYSTNKIPKTLTVSLSEENLSLLANSLKINVTQPKNGEQKSILQTVIDNARYALNTK", "aa_hexdigest": "0bb300c07fadff740364e38d732f90b3", "start_type": "TTG", "rbs_motif": null, "genes": [ "uvrC" ], "truncated": "both", "pseudogene": { "start": 161797, "stop": 163182, "observations": { "mutation": [ 161866, 162877 ], "stop": [ 161866, 162877 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75350", "query_cov": 1.0, "subject_cov": 0.5483333333333333, "identity": 0.997, "score": 619.0, "evalue": 4.72e-219, "gene_start": 1, "gene_end": 329, "reference_start": 32, "reference_end": 360, "reference_sequence": "MIIVNNTLAFKLKNAPHKPGCYLWKDDAGQVLYVGKAKDIFKRVHHYFNPNRSFKTRALVERIADVEYVILKNENDALNLEAKLIKQYKPRFNLVLKENNGYLYFFITASVKPTLELGRRYEFSKNKYFGPFASSKFRLRDIYDLLLKLFPLRKCAPHERGHPCFYYQLKMCMGQCMGEDTPERYQTTVKGIEQFFNHGPEQVLNHLQQQEIKASEQQNFEAARHFLDLQKAVLELVNMQQTAFIKAKQSHDFIGYVFEKNVLAITVFAYVDNQLIGKNQQVFELPQDDEKEVESALVTFIYHYYSTNKIPKTLTVSLSEENLSLLANSLKINVTQPKNGEQKSILQTVIDNARYALNTKWTGFINNLNRAEVHQQLAQLLQVPSIQSLEIIDISFYDKDHVVGAMLRYENGKRMKALSRRYNINIDHKGDTNYMADVVYRRIISSIQTHKQLPLSDLLIVDGGIAQINTVTKVFASFPNVTQPIIIGLAKNTRHQTDHIVLTDNTTINIDKNTPLFAYLTTIQEEVDSFAKHNAFKRVSRARFQNPLLQIEGVGRKTVQILLDNFQTMQNIEAASLSELSQFIPANLAQKIKTFLKEND" }, "paralog": false, "identity": 0.9934924078091106, "score": 871.0, "evalue": 5.92e-321, "description": "Internal stop codon at 161866, 162877. Nonsense mutation around 161866, 162877.", "psc": { "uniref90_id": "UniRef90_P75350", "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_P47448", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75350", "UniRef:UniRef50_P47448", "KEGG:K03703", "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P47448", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47448" ], "product": "UvrABC system protein C" } }, "id": "CNOCLNGIAL_323", "locus": "GCA000143945_00288" }, { "type": "cds", "sequence": "contig_1", "start": 162913, "stop": 163599, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14520.12", "PFAM:PF08459.17" ], "nt": "GTGCACCAGCAATTAGCGCAGCTTTTACAAGTTCCTTCGATTCAAAGTCTGGAAATTATTGACATCTCCTTTTATGACAAAGACCACGTGGTGGGGGCGATGTTACGGTACGAAAATGGCAAACGGATGAAAGCGTTATCACGCCGTTACAACATTAACATTGACCATAAGGGTGACACTAACTATATGGCTGATGTGGTGTACCGCCGGATTATTTCGTCCATCCAAACCCATAAACAGCTACCTTTAAGTGATCTTTTAATTGTCGATGGTGGGATTGCCCAAATTAATACGGTAACCAAGGTGTTTGCCTCTTTCCCTAACGTTACCCAACCGATCATTATTGGCTTAGCGAAAAACACCCGTCACCAAACTGACCACATTGTGTTAACGGATAACACTACCATTAACATTGACAAAAATACGCCTTTGTTTGCTTATCTTACCACCATTCAAGAAGAGGTCGATAGCTTTGCCAAACACAATGCCTTTAAGCGAGTTAGTCGCGCTCGCTTCCAAAATCCACTCTTACAAATTGAAGGGGTTGGGCGAAAAACTGTCCAAATATTACTTGATAATTTTCAAACAATGCAAAACATTGAAGCTGCTTCTTTGAGCGAATTATCACAGTTTATTCCTGCTAATTTAGCACAAAAAATTAAAACTTTTCTAAAGGAAAATGACTAA", "aa": "MHQQLAQLLQVPSIQSLEIIDISFYDKDHVVGAMLRYENGKRMKALSRRYNINIDHKGDTNYMADVVYRRIISSIQTHKQLPLSDLLIVDGGIAQINTVTKVFASFPNVTQPIIIGLAKNTRHQTDHIVLTDNTTINIDKNTPLFAYLTTIQEEVDSFAKHNAFKRVSRARFQNPLLQIEGVGRKTVQILLDNFQTMQNIEAASLSELSQFIPANLAQKIKTFLKEND", "aa_hexdigest": "89a07411c6a504027f0cf30b313274b6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14520.12", "name": "HHH_5", "length": 54, "aa_cov": 0.2236842105263158, "hmm_cov": 1.0, "evalue": 3.677398005136948e-08, "score": 29.2736759185791, "start": 176, "stop": 226 }, { "id": "PF08459.17", "name": "UvrC_RNaseH_dom", "length": 161, "aa_cov": 0.6710526315789473, "hmm_cov": 0.9751552795031055, "evalue": 5.1386133722195125e-34, "score": 112.88227081298828, "start": 7, "stop": 159 } ], "seq_stats": { "molecular_weight": 25918.44150000002, "isoelectric_point": 9.046477699279784 }, "id": "CNOCLNGIAL_324", "locus": "GCA000143945_00289" }, { "type": "cds", "sequence": "contig_1", "start": 163652, "stop": 164071, "strand": "+", "frame": 2, "gene": "yfcE", "product": "putative metallophosphoesterase MPN_126", "db_xrefs": [ "COG:COG0622", "COG:R", "EC:3.1.4.-", "GO:0016787", "GO:0046872", "KEGG:K07095", "SO:0001217", "UniRef:UniRef50_P47449", "UniRef:UniRef90_P75349" ], "nt": "ATGAAGTTGCACAACCCTGATGTAGTAATTCATGCTGGTGATCATTTAACTACTAAAAAGTTTATGGATCAAAATGCCACTTTTTGGGTTGCTGGTAATCATGATGTTGTTGGCGAAGAGATTCAAATGTTTGAGTTAGAGGGAATCCAGTTTGTTTTAATGCACGGCCATCAAGCGCCCCGTCATGATCTCAAGCAGTGGTATAAAATGCTGGTTGATCAAGCTAAAAGCTATCTTTGTGATGTTTTAATTGTGGGTCACAGTCACATAGAGCACTATGAAACTATTGATGGGATCCAGGTTATTAACCCCGGTTCTCTAGAAATTCCCCGTAATCCGAGAAAGCTACCTACCTACTGTAATTTAAACTTAAGTCAGGGCAGGATAAGTGATTTAACGTTCCATTTTCCTCGGGATTAA", "aa": "MKLHNPDVVIHAGDHLTTKKFMDQNATFWVAGNHDVVGEEIQMFELEGIQFVLMHGHQAPRHDLKQWYKMLVDQAKSYLCDVLIVGHSHIEHYETIDGIQVINPGSLEIPRNPRKLPTYCNLNLSQGRISDLTFHFPRD", "aa_hexdigest": "a7871139f2662e9bb2626c69be59a706", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75349", "query_cov": 1.0, "subject_cov": 0.8742138364779874, "identity": 1.0, "score": 297.0, "evalue": 5.05e-102, "valid": true, "gene": "YfcE", "product": "Probable metallophosphoesterase MPN_126", "ec_ids": [ "3.1.4.-" ], "uniref50_id": "UniRef50_P47449", "cog_id": "COG0622", "cog_category": "R", "kegg_orthology_id": "K07095", "go_ids": [ "GO:0016787", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47449", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47449" ], "product": "Probable metallophosphoesterase MG207" }, "genes": [ "yfcE" ], "id": "CNOCLNGIAL_325", "locus": "GCA000143945_00290" }, { "type": "cds", "sequence": "contig_1", "start": 164519, "stop": 165235, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22", "PFAM:PF23991.1", "PFAM:PF03670.18" ], "nt": "GTGACGAACCACCTTCTTAAACATTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTGCCATTAAACGCATAATTTCACTTGCTTTGTTTAAGAAAAGACTTAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGGATTTAATGGCAATTTCAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAACAATCAGCGTCTCATAAAGATTGAAACTACTTTGGCTATCCAAGGCGAACAAATCAACAAATTGACTCAAACTGTTGAAAAGCAAGGCGAACAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCAGGGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAGCACAAAGACAAGAGTTTAATGCCCGCATGGATCGTTTGGAAAATCTTTTGGTGGAAAGTATAGAATCTACCAATAATCGCTTCGACTCTATGGAAAGACGTTTAGACTCTATGGATAGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTTGTCTTGATTTTGTTGAAGGACGCTTAGACTCTATGGAAACTCGTTTAGACTCTATGGAAACTCGCCTGGACAAAGTCGATCCGCCCAAATAG", "aa": "MTNHLLKHFYVFPINNKGGLAIKRIISLALFKKRLNKDKINNCHVWEEELPDGSYDMGFNGNFNHMEKRKSGYVTQKQFSEFKDANNQRLIKIETTLAIQGEQINKLTQTVEKQGEQINQLVQVVLLQGEQIRELQVEQKAQRQEFNARMDRLENLLVESIESTNNRFDSMERRLDSMDSRLDSMENRLDSMEGRLDSMENRLDSMEGCLDFVEGRLDSMETRLDSMETRLDKVDPPK", "aa_hexdigest": "42e82bd6e17bc9942d962463fcf301dd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 68, "aa_cov": 0.27310924369747897, "hmm_cov": 1.0, "evalue": 7.857949961615958e-39, "score": 127.8681411743164, "start": 59, "stop": 123 }, { "id": "PF23991.1", "name": "DUF7310", "length": 68, "aa_cov": 0.2857142857142857, "hmm_cov": 0.9852941176470589, "evalue": 3.042223563920448e-12, "score": 42.36927795410156, "start": 165, "stop": 232 }, { "id": "PF03670.18", "name": "UPF0184", "length": 33, "aa_cov": 0.13865546218487396, "hmm_cov": 1.0, "evalue": 9.101681687698395e-06, "score": 21.528133392333984, "start": 166, "stop": 198 } ], "seq_stats": { "molecular_weight": 27864.25610000001, "isoelectric_point": 5.437809181213378 }, "id": "CNOCLNGIAL_326", "locus": "GCA000143945_00291" }, { "type": "cds", "sequence": "contig_1", "start": 165492, "stop": 165773, "strand": "+", "frame": 3, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "RefSeq:WP_015344878.1", "SO:0001217", "UniParc:UPI0002B84587", "UniRef:UniRef100_A0AAP8E4D3", "UniRef:UniRef50_A0AAP8E4D3", "UniRef:UniRef90_A0AAP8E4D3" ], "nt": "ATGAGTAATACAAAAAAATTAAGAATAACTTCAATCGAAGAAACAATAGAAATAATAGTTAATTCAAAACTATTAGCTTTTGGTTTTTACCCAGTTGGTTTAGAAGATGGAACTTACGAACTTAATGTAAGGATTATTTTTAAAGAAAAAACTGCAGATTCTGAGAAGAGTAGCAGTGTACATCCTTTACAAAAGGAGTATTATGTAAGGGAATTTTTGAGATTTCTTGAGCGCCATAAAGATTCTCTGGCGCAACAACTATTAGTAGTAGAAGACAATTAA", "aa": "MSNTKKLRITSIEETIEIIVNSKLLAFGFYPVGLEDGTYELNVRIIFKEKTADSEKSSSVHPLQKEYYVREFLRFLERHKDSLAQQLLVVEDN", "aa_hexdigest": "6535a3d15727147d2970eafc4bef2012", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B84587", "ncbi_nrp_id": "WP_015344878.1", "uniref100_id": "UniRef100_A0AAP8E4D3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B84587", "RefSeq:WP_015344878.1", "UniRef:UniRef100_A0AAP8E4D3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E4D3", "uniref90_id": "UniRef90_A0AAP8E4D3" }, "psc": { "uniref90_id": "UniRef90_A0AAP8E4D3", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D3" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D3" ], "product": "Family K-like protein" }, "genes": [], "id": "CNOCLNGIAL_327", "locus": "GCA000143945_00292" }, { "type": "cds", "sequence": "contig_1", "start": 166026, "stop": 166283, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGAACCTGCGCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNSAKGITAGSGSQQTTYEPARTEAALTASTTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "54aa34d2360fc2a5f30de0b05c27255a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 2.638322400798446e-09, "score": 32.272003173828125, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9074.855599999997, "isoelectric_point": 8.138311576843265 }, "id": "CNOCLNGIAL_328", "locus": "GCA000143945_00293" }, { "type": "cds", "sequence": "contig_1", "start": 166314, "stop": 166907, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_128", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75347", "UniRef:UniRef90_P75347" ], "nt": "GTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGAGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATCCAGCAAGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGAAGGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCCTTTAA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRELGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNPASPTQGSRLRHHPIQFGIWSIRHPHPLKAVACDRANSQGPPQMIRLDPHSVRCALCL", "aa_hexdigest": "685101d07832070a93dbdfcf293db11c", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 166014, "stop": 166907, "observations": { "mutation": [ 166281 ], "stop": [ 166281 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75347", "query_cov": 1.0, "subject_cov": 0.5225464190981433, "identity": 0.995, "score": 379.0, "evalue": 2.56e-130, "gene_start": 1, "gene_end": 197, "reference_start": 181, "reference_end": 377, "reference_sequence": "MISPKLDWLILTATASLAAGLTVVGHFTSTTTTLKRQQFSYTRPDEVALRHTNAINPRLTPWTYRNTSFSSLPLTGENPGAWALVRDNSAKGITAGSGSQQTTYEPARTEAALTASTTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKNKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRELGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNPASPTQGSRLRHHPIQFGIWSIRHPHPLKAVACDRANSQGPPQMIRLDPHSVRCALCL" }, "paralog": false, "identity": 0.9966329966329966, "score": 592.0, "evalue": 2.74e-216, "description": "Internal stop codon at 166281. Nonsense mutation around 166281.", "psc": { "uniref90_id": "UniRef90_P75347", "product": "Uncharacterized protein MPN_128", "uniref50_id": "UniRef50_P75347", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75347", "UniRef:UniRef50_P75347" ] }, "pscc": { "uniref50_id": "UniRef50_P75347", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75347" ], "product": "Uncharacterized protein MPN_128" } }, "id": "CNOCLNGIAL_329", "locus": "GCA000143945_00294" }, { "type": "cds", "sequence": "contig_1", "start": 167607, "stop": 167963, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_129", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75346", "UniRef:UniRef90_P75346" ], "nt": "ATGGTCTCTTTTTTTGATATATATACCAAAGCATCGATTGTAGAAGCTGTACGTTTTTCTTTTTTGAATACCTTTCAATTAAAGGAACTTGGTTTACCGATTAAAGAAATTGCTGTACCATTAGGCACATTAGTCTTTCTTTTTGTTGTTATTATTACCTTAATTCCGTTGTTAATTATTGGTAATTTAATTTGGACTAACTTGCGTTTAATAGAACGGGAAAATACACAGCAGTACCAACTCGTTTTTGGTTACAGTTTGATAGTTAGTGATATTGTTGGTTTTGCTATTGTTTTCTTCGGTGCCATTTTAGGTACAAATTTAAAAAGTGTTGAATTATTCATAGCATTGGGTTGA", "aa": "MVSFFDIYTKASIVEAVRFSFLNTFQLKELGLPIKEIAVPLGTLVFLFVVIITLIPLLIIGNLIWTNLRLIERENTQQYQLVFGYSLIVSDIVGFAIVFFGAILGTNLKSVELFIALG", "aa_hexdigest": "cd2e740ca967c78b750e6197fc5c17b1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 167607, "stop": 168056, "observations": { "mutation": [ 167961 ], "stop": [ 167961 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75346", "query_cov": 1.0, "subject_cov": 0.7919463087248322, "identity": 1.0, "score": 214.0, "evalue": 9.18e-70, "gene_start": 1, "gene_end": 118, "reference_start": 1, "reference_end": 118, "reference_sequence": "MVSFFDIYTKASIVEAVRFSFLNTFQLKELGLPIKEIAVPLGTLVFLFVVIITLIPLLIIGNLIWTNLRLIERENTQQYQLVFGYSLIVSDIVGFAIVFFGAILGTNLKSVELFIALGWMMMLGSLIALGTTANLVSSIYLYIKLALKR" }, "paralog": false, "identity": 0.9865771812080537, "score": 277.0, "evalue": 7.11e-97, "description": "Internal stop codon at 167961. Nonsense mutation around 167961.", "psc": { "uniref90_id": "UniRef90_P75346", "product": "Uncharacterized protein MPN_129", "uniref50_id": "UniRef50_P75346", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75346", "UniRef:UniRef50_P75346", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75346", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75346" ], "product": "Uncharacterized protein MPN_129" } }, "id": "CNOCLNGIAL_330", "locus": "GCA000143945_00295" }, { "type": "cds", "sequence": "contig_1", "start": 168039, "stop": 168446, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ], "nt": "GTGGTTTTCGTCACTTTGTCCGTTCTTATCATTCCTTCACCCCGCGCCGGTTCAGAGCAGGAGGTTGGCTTGGTCGAGGGTGGGTCATTTGGAGCCGCCTGTAGTCGTCTTCTGACTGCCACCAAGCGTGGCTTGGACGTTGGCTTTGAGGCCAACGCCGACATTCGAGAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTTAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTCCCATACCTGTACCCATTCCCAATCCAGGGCACATAATCCAACACCTAGTCGAGAACCGACCCCGCGAGGTTGAACCCGGTGGAGAAGCGCGTGAGCGTGGGGGAGAAGTGTGTCTATCTCTTCAATGCTAG", "aa": "MVFVTLSVLIIPSPRAGSEQEVGLVEGGSFGAACSRLLTATKRGLDVGFEANADIRERREKGTRTTCVRTSSAGRPRAGFRRGYIIHAPVVIPIPVPIPNPGHIIQHLVENRPREVEPGGEARERGGEVCLSLQC", "aa_hexdigest": "67335385041c51049ca065e41dce58c8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "query_cov": 0.9851851851851852, "subject_cov": 0.9432624113475178, "identity": 0.812, "score": 203.0, "evalue": 2.6e-65, "valid": false, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14521.533299999986, "isoelectric_point": 9.238915824890135 }, "id": "CNOCLNGIAL_331", "locus": "GCA000143945_00296" }, { "type": "cds", "sequence": "contig_1", "start": 169025, "stop": 169222, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0AAN4XEA6" ], "nt": "ATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTTTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGCTGACACTGAGACGACAAATAAGGAAAAGCCTTTAGGCACTTGA", "aa": "MKKVKALRNLFLEKASEKLEYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVADTETTNKEKPLGT", "aa_hexdigest": "8f5463db56fabcd688e705e868eeecf6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XEA6", "query_cov": 1.0, "subject_cov": 0.8552631578947368, "identity": 0.985, "score": 124.0, "evalue": 3.56e-36, "valid": true, "uniref50_id": "UniRef50_A0A0H3DJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7412.577499999999, "isoelectric_point": 7.800045967102052 }, "id": "CNOCLNGIAL_332", "locus": "GCA000143945_00297" }, { "type": "cds", "sequence": "contig_1", "start": 169363, "stop": 169620, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D96482" ], "nt": "GTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAATGTCAACGGCACCTTAAGTGGTTCGCGAACTACGCCTAATCAGCAAGGCACTCCCTGGTTAACCCTCGACCAAGCCAACCTCCAGCTCTGA", "aa": "MLDYVPWIGNGYRYGNNHRGSNSSTSGVTTQGQSQNASSNEPAPTFSNVGVGLKANVNGTLSGSRTTPNQQGTPWLTLDQANLQL", "aa_hexdigest": "f682510355e5f0014a5647b598ae3463", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002D96482", "query_cov": 1.0705882352941176, "subject_cov": 0.8504672897196262, "identity": 0.593, "score": 95.9, "evalue": 5.27e-24, "valid": false, "uniref50_id": "UniRef50_UPI0002D96482" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D96482", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D96482" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8982.590799999998, "isoelectric_point": 8.209807014465333 }, "id": "CNOCLNGIAL_333", "locus": "GCA000143945_00298" }, { "type": "cds", "sequence": "contig_1", "start": 169911, "stop": 170234, "strand": "+", "frame": 3, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6", "UniRef:UniRef90_A0AB33HTF6" ], "nt": "GTGCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTCAATAAGAGTGGGCAAGATGATAACAGTAAGTTTAATTCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGATTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCTTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCAGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTLTSSTANQNSTTVPSIGFKIPEQNNDSKAVLITPGLA", "aa_hexdigest": "bec1a9e68a7a9049e29073db24c1c2a0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 169878, "stop": 170537, "observations": { "mutation": [ 170232, 170376 ], "stop": [ 170232, 170376 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HTF6", "query_cov": 1.0, "subject_cov": 0.4863636363636364, "identity": 0.991, "score": 207.0, "evalue": 3.23e-66, "gene_start": 1, "gene_end": 107, "reference_start": 12, "reference_end": 118, "reference_sequence": "MSFTNKNNAQRVQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTLTSSTANQNSTTVPSIGFKIPEQNNDSKAVLITPGLAWTPQDVGNLVVSGTSLSFQLGGWLVTVHGLYQTPRGLFGPPVKWPGCQWQRPKGVNLGQAALSGISWQLGQPVGTGRECVGFEGGVGGSSSQRGVRVASSYK" }, "paralog": false, "identity": 0.9863013698630136, "score": 437.0, "evalue": 3.03e-159, "description": "Internal stop codon at 170232, 170376. Nonsense mutation around 170232, 170376.", "psc": { "uniref90_id": "UniRef90_A0AB33HTF6", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HTF6", "UniRef:UniRef50_A0AB33HTF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6" ], "product": "Adhesin P1 homolog" } }, "id": "CNOCLNGIAL_334", "locus": "GCA000143945_00299" }, { "type": "cds", "sequence": "contig_1", "start": 170449, "stop": 171096, "strand": "+", "frame": 1, "gene": null, "product": "Putative adhesin P1-like protein MPN_132", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75266", "UniRef:UniRef90_P75266" ], "nt": "TTGGGACGGGTCGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGACACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTCCACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGCTCCGGTCAAACCAATTCCCAAAACACCTCCCCCTACCTGCATTTGATTAAACCTAAAAAAGTTACTGCTTCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGGGTGAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTGTTTGGGACGAATAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGTACCACGCAGGACTCAAGCACCACCAATCAACTGTCACCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGAGGTGGTGGGGGTGGGAGATTTGTCTAAGCGAGCTTCCATTGAATCAAGTCGTTTGTGA", "aa": "MGRVESVWDLKGVWADQAHSAVSESQAATSSTTTTATGDTLPEHPNALAYQISSTDKDSYKASTQGSGQTNSQNTSPYLHLIKPKKVTASDKLDDDLKNLLDPNEVRVKLRQSFGTDHSTQPQPQALKTTTPVFGTNSGNLGSVLSGGGTTQDSSTTNQLSPVERVSGWLVGQLPSTSDGNTSSTNNLAPNTNTGNEVVGVGDLSKRASIESSRL", "aa_hexdigest": "95c1d095eecced9c485ca5da488a3b19", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75266", "query_cov": 1.0093023255813953, "subject_cov": 0.8410852713178295, "identity": 0.982, "score": 397.0, "evalue": 1.21e-138, "valid": true, "product": "Putative adhesin P1-like protein MPN_132", "uniref50_id": "UniRef50_P75266" }, "pscc": { "uniref50_id": "UniRef50_P75266", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75266" ], "product": "Putative adhesin P1-like protein MPN_132" }, "genes": [], "id": "CNOCLNGIAL_335", "locus": "GCA000143945_00300" }, { "type": "cds", "sequence": "contig_1", "start": 171572, "stop": 172048, "strand": "+", "frame": 2, "gene": "yncB", "product": "putative lipo MG186-like protein", "db_xrefs": [ "COG:COG1525", "COG:L", "GO:0004519", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75265", "UniRef:UniRef90_P75265" ], "nt": "ATGAAAGGGTTTTCTTGCTCCAGACCGGGCTATCTTACTGGTCTCTTGCTCCTCGCTGTAGCCCCTATTCTCACTGCTTGTACCCGCGACTATACTACCAAGAACGAATTTCAATTAACAACAGCTCAACAGGCCAAATTAAAGCCAGCCACCATCGAATATTGGCGTGATGGTGATACGCCTGAGATCAATTACGCAAGCGAAGAGCGCCGAAAGGAAGCTGAACAAAAATCTAAAGAAAACGCTAAGAAAGAAGACAAAAAAGAGGAAAAGAAAACTGAAGATTCCCAAGATTCTTCTTCTGCATCCACGCAAGTTCGTAGTAGTAAACACGGTCTCCGCATCTATGGAATAGATACACCCGAAAAACATGTAAGTAGCAAAGGCGATAGCACCGGTGATGAAAAAATCGAAGCCGAAAAGGCCTCTAACTATGCTGAAAAGCTAATCCCTAAAGGCAGTACTGTTTGGGTTTGA", "aa": "MKGFSCSRPGYLTGLLLLAVAPILTACTRDYTTKNEFQLTTAQQAKLKPATIEYWRDGDTPEINYASEERRKEAEQKSKENAKKEDKKEEKKTEDSQDSSSASTQVRSSKHGLRIYGIDTPEKHVSSKGDSTGDEKIEAEKASNYAEKLIPKGSTVWV", "aa_hexdigest": "8111908103a440578a0df78a47b4c3c9", "start_type": "ATG", "rbs_motif": null, "genes": [ "yncB" ], "truncated": "3-prime", "pseudogene": { "start": 171572, "stop": 172351, "observations": { "mutation": [ 172046 ], "stop": [ 172046 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75265", "query_cov": 1.0, "subject_cov": 0.5249169435215947, "identity": 1.0, "score": 257.0, "evalue": 6.1e-84, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MKGFSCSRPGYLTGLLLLAVAPILTACTRDYTTKNEFQLTTAQQAKLKPATIEYWRDGDTPEINYASEERRKEAEQKSKENAKKEDKKEEKKTEDSQDSSSASTQVRSSKHGLRIYGIDTPEKHVSSKGDSTGDEKIEAEKASNYAEKLIPKGSTVWVWSLNTYSYDREVGALFFKSNPKQTFFQSFEVAMVEAGHAIPIAGTGLNLIADPELSADDPLSVIGLQLANAANKAYNAKINIWSHDTDGYRSLTAVYKLRGADISWTRFLDEANGYSSASAGTGASLYQLWDQRQAKLAQKGS" }, "paralog": false, "identity": 0.9961389961389961, "score": 458.0, "evalue": 1.71e-165, "description": "Internal stop codon at 172046. Nonsense mutation around 172046.", "psc": { "uniref90_id": "UniRef90_P75265", "gene": "YncB", "product": "Uncharacterized lipoprotein MG186 homolog", "uniref50_id": "UniRef50_P75265", "cog_id": "COG1525", "cog_category": "L", "go_ids": [ "GO:0005886", "GO:0004519" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75265", "UniRef:UniRef50_P75265", "COG:COG1525", "COG:L", "GO:0005886", "GO:0004519" ] }, "pscc": { "uniref50_id": "UniRef50_P75265", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75265" ], "product": "Uncharacterized lipoprotein MG186 homolog" } }, "id": "CNOCLNGIAL_336", "locus": "GCA000143945_00301" }, { "type": "cds", "sequence": "contig_1", "start": 172079, "stop": 172363, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCGCTTTATTCTTTAAGTCTAACCCTAAGCAAACCTTCTTCCAATCCTTTGAAGTAGCTATGGTTGAGGCTGGTCATGCTATTCCAATTGCTGGGACTGGTTTAAATTTAATCGCTGATCCGGAATTAAGTGCTGATGATCCTTTAAGTGTCATTGGTCTACAGTTAGCCAACGCTGCCAATAAGGCTTACAACGCTAAAATTAACATTTGGAGCCATGACACTGATGGTTATCGTTCCTTGACTGCTGTTTACAAGTTAAGGGGCGCTGATATTAGTTGA", "aa": "MGALFFKSNPKQTFFQSFEVAMVEAGHAIPIAGTGLNLIADPELSADDPLSVIGLQLANAANKAYNAKINIWSHDTDGYRSLTAVYKLRGADIS", "aa_hexdigest": "c9560ff980c34fa65a91b6462f8af8b7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10070.29969999999, "isoelectric_point": 5.466001319885254 }, "id": "CNOCLNGIAL_337", "locus": "GCA000143945_00302" }, { "type": "cds", "sequence": "contig_1", "start": 172501, "stop": 172845, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU7Q6T1" ], "nt": "ATGGAAAAGATACAAGCGGAAAAATCCCAAAGTGCAATTGAGTTTAAGAATATTGTAGTCGACTTTGGTGAGTCAATCGCAATTGATAACATTAACCTCACGGTTAAAAAGAAGGAGTTAGTTACCTTACTAGGTCCTTCTGGGTGTGGGAAAACAACTTCGCTTTCCGTGATTGCGGGTCTTATCGCCCCTACTAGTGGTCAGGTTTTATTTAATGGTTATGATGTCACAAAAAAGCCACCACAACAACGCAAACTGGGATTAGTGTTCCAAAACTATGCCTTGTATCCGCACATGAGTGTGTTTGAAAACATTGTCTTCCCACTCTACAGTGATACGTCTTGA", "aa": "MEKIQAEKSQSAIEFKNIVVDFGESIAIDNINLTVKKKELVTLLGPSGCGKTTSLSVIAGLIAPTSGQVLFNGYDVTKKPPQQRKLGLVFQNYALYPHMSVFENIVFPLYSDTS", "aa_hexdigest": "875cdab9b3219eea9ca66ce6958763fc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAU7Q6T1", "query_cov": 0.8859649122807017, "subject_cov": 0.8938053097345132, "identity": 0.505, "score": 116.0, "evalue": 1.69e-31, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0AAU7Q6T1" }, "pscc": { "uniref50_id": "UniRef50_A0AAU7Q6T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU7Q6T1" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "CNOCLNGIAL_338", "locus": "GCA000143945_00303" }, { "type": "cds", "sequence": "contig_1", "start": 172897, "stop": 173265, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCTTAAAGGCTAATGGTGCTACTAGTGAGGAGCTGGCAGAGCTCAATCGTTTAATGCAACAACGAATCGATGAGCCCAAACGAATGGCTTACCAGATTAATGATTTGATGGTAAGTGTGTTTCAAAAACAGAGTGAACTGGAAGCTAACCTAAAGTTAATTCCCCGCAAAAAACAGTTTGCCATTATTTCACTCAGCAAGGAAACACTAAGTCAAATAAGGGATGTTGAAACTAAAGCCAAGGCTGCATTGGAAACCGCTGATAGTGCTGAAGTGGAGCAGACCATTAAGAGTGAACTTAAGCAAAAACTAAGTGAAATTAAGGCTAATTACCACGATGAAAAGGCCAACATTAAAGCTTACTGA", "aa": "MILKANGATSEELAELNRLMQQRIDEPKRMAYQINDLMVSVFQKQSELEANLKLIPRKKQFAIISLSKETLSQIRDVETKAKAALETADSAEVEQTIKSELKQKLSEIKANYHDEKANIKAY", "aa_hexdigest": "8e8b9dee8047eaeb172646f3bb40250d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13922.866499999982, "isoelectric_point": 7.878310585021973 }, "id": "CNOCLNGIAL_339", "locus": "GCA000143945_00304" }, { "type": "cds", "sequence": "contig_1", "start": 173272, "stop": 173361, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGCCAATATTAAGACCGAATTAAAAACCGAAAAAACTGCCATTAAGCAAACTAATGACTATGCCAAATTGAAGGAATTGAAGTGA", "aa": "MLANIKTELKTEKTAIKQTNDYAKLKELK", "aa_hexdigest": "e1eaa73ae50a081dc00917ea6413f12d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3364.949300000001, "isoelectric_point": 9.523737144470214 }, "id": "CNOCLNGIAL_340", "locus": "GCA000143945_00305" }, { "type": "cds", "sequence": "contig_1", "start": 173380, "stop": 173949, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGAATTTAAAGAAGCAGTACCGCAGTTACTTTAAACAACTAAAAGCCAAATACAGCTTAAAAGATGGCAATTTAACTGAAAGCGAATTAAGTCAAATTGAGGAGTTGCAAAAACGGATTGTTTCGCTCAAAGATTTCATTAACCGCACTGCTAAAGAAGTAGCTGAAAAACTGGAAATTACTAAGATTCTCCACAAGCGCCCCGCTAATATTTCTGGGGGTCAACAACAACGGGTAGCTATTGCTAGAGCAATTGTTAGAAGACCTAAGGTTCTACTTATGGACGAACCGTTGTCCAACTTAGATGCGAAGTTGCGGGTACAAACACGGCAATGGATCCGTAAGTTCCAGCAAGACCTGCAAATTACTACTGTCTTTGTGACTCACGATCAGGAAGAGGCAATGAGTATTAGCGACACGATTGTCTGCATGTCAACTGGTAAGGTACAGCAAATAGGCTCCCCTTCCGAGTTGTACTTAAAACCGGCCAATGAGTTCGTTGCTACCTTTTTGGGTAGCCCGGAAATGAATATCGTCAACGCTACGGTTAAAGCTGGACAGCTGTTGTGA", "aa": "MNLKKQYRSYFKQLKAKYSLKDGNLTESELSQIEELQKRIVSLKDFINRTAKEVAEKLEITKILHKRPANISGGQQQRVAIARAIVRRPKVLLMDEPLSNLDAKLRVQTRQWIRKFQQDLQITTVFVTHDQEEAMSISDTIVCMSTGKVQQIGSPSELYLKPANEFVATFLGSPEMNIVNATVKAGQLL", "aa_hexdigest": "fc9d91ecccb12c6f47af9d5cf661e7e6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 57, "aa_cov": 0.2804232804232804, "hmm_cov": 1.0, "evalue": 1.2282106410809805e-14, "score": 50.5290412902832, "start": 46, "stop": 98 } ], "seq_stats": { "molecular_weight": 21531.76039999999, "isoelectric_point": 9.680137443542481 }, "id": "CNOCLNGIAL_341", "locus": "GCA000143945_00306" }, { "type": "cds", "sequence": "contig_1", "start": 174079, "stop": 174261, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAACCTAGGGAAACACATGGTTGGGGTTGTGGAGAGCAATGGGGTACAGCTCAATGTGCGCTTAGAATTGAGCCACCAGTTTGAAGTAGGTAATGCAGTTAAGTTTACGATTAAGCCTAATGGCTTACACTTCTTTGATCCCCAAACAACGCAACGGGTGGAGGTAAAACATGTTTAA", "aa": "MENLGKHMVGVVESNGVQLNVRLELSHQFEVGNAVKFTIKPNGLHFFDPQTTQRVEVKHV", "aa_hexdigest": "d0b0d10ab17a162e6af31251d2a1c603", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6784.6942, "isoelectric_point": 6.901981544494629 }, "id": "CNOCLNGIAL_342", "locus": "GCA000143945_00307" }, { "type": "cds", "sequence": "contig_1", "start": 174344, "stop": 174628, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTGTTTTGCCCAGCTTTATTAACTACCTTTCTCTTTACTTTAGTACCGTTCTTCCTTACGTTACAGAAAGGGTTTAGTCACAATGAGGACATCTACCGGGTTGATTCCCAACAGTTTGGCTTTCAAACTTTCGCTAACCTTTTTAGTGAGTCCAACTTTATTTTGGGCTTGCGCAATTCCTTTTTGTACTCGATTATTTCCCTACCACTGACAATTGTGCTAGCAATTATTATTTCTAGTGCCATTGTCTTTGTGTACCGGAAGTTAGCACGGGGCTTTTGA", "aa": "MLFCPALLTTFLFTLVPFFLTLQKGFSHNEDIYRVDSQQFGFQTFANLFSESNFILGLRNSFLYSIISLPLTIVLAIIISSAIVFVYRKLARGF", "aa_hexdigest": "c7d05f9b2e85e6f87ed8946a8956561a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10754.560499999989, "isoelectric_point": 9.032939338684084 }, "id": "CNOCLNGIAL_343", "locus": "GCA000143945_00308" }, { "type": "cds", "sequence": "contig_1", "start": 174695, "stop": 175189, "strand": "+", "frame": 2, "gene": "ugpA", "product": "putative ABC transporter permease MG188-like protein", "db_xrefs": [ "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75263", "UniRef:UniRef90_P75263" ], "nt": "TTGGACTCCTCTTCGGGAATCTTAAACAACATCTTTCATGTCAACATTAAGTGGTTAGATTCGGGGGAACGTGATACCTTTAATGCCTTATGGGGAATTCTTATCTTTGGAATTTGGAAGAACATGGCTTTTAATGTGCTGGTAATATCAACCGCCATGTTAAGTGTGGATCCTACTTTATATAAGGTAGCTAACTTAGATGGTGCAAAACCAATTAGGCAGTTCTTTAAGATTACCTTACCTTCAATCCGCCCAACCTTAATTTTCCTGTTGACCCTGTTAATTTTGGGCGGAATGCAGGTGTTTCCAATTTCGTTGTTTAACGGGAATGATAGTGAAGCTGTAACCAATGGGGGTTCGACCATCTTGCTTTACATCTTCCAAAAGATTCGTGATCAGAATAACAATTTTGCTGGTGCTGCTACCTTGGTGTTGTTCATCTTGGGGGTTTGTTATGGGTTAGTGTTGCGCAATGGTTTTCGCTTAATTGAATGA", "aa": "MDSSSGILNNIFHVNIKWLDSGERDTFNALWGILIFGIWKNMAFNVLVISTAMLSVDPTLYKVANLDGAKPIRQFFKITLPSIRPTLIFLLTLLILGGMQVFPISLFNGNDSEAVTNGGSTILLYIFQKIRDQNNNFAGAATLVLFILGVCYGLVLRNGFRLIE", "aa_hexdigest": "d7c01eccbfee5aff83d8ab306d5babb7", "start_type": "TTG", "rbs_motif": null, "genes": [ "ugpA" ], "truncated": "both", "pseudogene": { "start": 174395, "stop": 175243, "observations": { "mutation": [ 174626, 175187, 175196 ], "stop": [ 174626, 175187, 175196 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75263", "query_cov": 1.0, "subject_cov": 0.49848024316109424, "identity": 0.9940000000000001, "score": 323.0, "evalue": 2.94e-109, "gene_start": 1, "gene_end": 164, "reference_start": 148, "reference_end": 311, "reference_sequence": "MFKWLLKHRSKPTPLELGIFDQKVSFWKPFLLFCPALLTTFLFTLVPFFLTLQKGFSHNEDIYRVDSQQFGFQTFANLFSESNFILGLRNSFLYSIISLPLTIVLAIIISSAIVFVYRKLARGFWQTVFFLPYVTSGVAVSIAFIYILDSSSGILNNIFHVNIKWLDSGERDTFNALWGILIFGIWKNMAFNVLVISTAMLSVDPTLYKVANLDGAKPIRQFFKITLPSIRPTLIFLLTLLILGGMQVFPISLFNGNDSEAVTNGGSTILLYIFQKIRDQNNNFAGAATLVLFILGVCYGLVLRNGFRLIEWAQWKIKRHYVQTKLNLV" }, "paralog": false, "identity": 0.9893617021276596, "score": 541.0, "evalue": 1.14e-197, "description": "Internal stop codon at 174626, 175187, 175196. Nonsense mutation around 174626, 175187, 175196.", "psc": { "uniref90_id": "UniRef90_P75263", "gene": "UgpA", "product": "Probable ABC transporter permease protein MG188 homolog", "uniref50_id": "UniRef50_P75263", "cog_id": "COG1175", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75263", "UniRef:UniRef50_P75263", "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75263" ], "product": "Probable ABC transporter permease protein MG188 homolog" } }, "id": "CNOCLNGIAL_344", "locus": "GCA000143945_00309" }, { "type": "cds", "sequence": "contig_1", "start": 175314, "stop": 175601, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTAATCTTTAAAACGGTGGTGTTGTGCTTCTTTGGTTTAATGGTCATCTTTCCCTTTTATTTGATGCTGGTGGTAGCACTCACCAGTGATGAAGTGGTCTTAAACATTCGTGAACCAATTCTCAAATCCGATGGGTGGCACTTCGAGAACTTCCGCCGTGTTTTGGAAGATGGTAAATACCTTAATGCCATTTGGATTAACAGCTTAGTAACAATTTTGTCGATTATCCTACGGCTTTTCTTTACCGTTAGCATGGGCTATGCCTTTTCGTTAAAAAAGTGA", "aa": "MGLIFKTVVLCFFGLMVIFPFYLMLVVALTSDEVVLNIREPILKSDGWHFENFRRVLEDGKYLNAIWINSLVTILSIILRLFFTVSMGYAFSLKK", "aa_hexdigest": "c875a6912b1c351228925538ae0827fe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11027.184199999987, "isoelectric_point": 8.888788032531739 }, "id": "CNOCLNGIAL_345", "locus": "GCA000143945_00310" }, { "type": "cds", "sequence": "contig_1", "start": 175743, "stop": 175898, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTGTCGCTTCTGTGTTCTCTGGGTTTATGTTCCGTACTGCCTTTGAAGCAATTCCACCACGAATTAAAGAATCGGCCTTTGTTGATGGTTGTACAGGGTTGAGGTACTTCCTCAAAATTGCCTTTCCAATGGTACGTAGTACCACTTGA", "aa": "MPFVASVFSGFMFRTAFEAIPPRIKESAFVDGCTGLRYFLKIAFPMVRSTT", "aa_hexdigest": "0a0d9ade4e383682e19d32f35a104e26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5739.753599999999, "isoelectric_point": 9.775937461853026 }, "id": "CNOCLNGIAL_346", "locus": "GCA000143945_00311" }, { "type": "cds", "sequence": "contig_1", "start": 175953, "stop": 176174, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTACTAACAAACAGACCCGATCTCAACATTAACCTGTGGGTGTTGGCGCAAGGGACTGATGGCAATGCCGGGCAAAGTGACGAACAAATCCGGGTGTTGTTGAATTTAAAGATGGCAGCAGCCATTTTAGCAATTCTGCCAATGTTTATTGTTTACTTCTTGTTTAGGAAAAGGATTATGAAAGCCGTGGGATCACGTGCCAATACGATTAAAGGATAG", "aa": "MLLTNRPDLNINLWVLAQGTDGNAGQSDEQIRVLLNLKMAAAILAILPMFIVYFLFRKRIMKAVGSRANTIKG", "aa_hexdigest": "f555fe81ac1cf6d78dcb039d05c4ddb1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8129.638099999993, "isoelectric_point": 10.519902610778807 }, "id": "CNOCLNGIAL_347", "locus": "GCA000143945_00312" }, { "type": "cds", "sequence": "contig_1", "start": 176752, "stop": 177159, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22", "PFAM:PF23991.1" ], "nt": "GTGAACACGGAATACGTAACGCGCAAAGAGTTTAACGAGTACAAAGACTCAAACGACCAACGACTCATAAAGATTGAAACCACTTTGGCTGCCCAAGGTGAACAAATAAGAATCCAGGGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAGCGCAAAGACAAGAGTTTAACGCCCGCATGGATCGTTTGGAAAACCTTTTGGTTGAAAGTATAGAATCCACCAATAAACGCTTCGACTCTATTGAAGGTCGTCTAGACTCTATGGATAGTCGTCTTGATTCTATGGAAAATCGCTTGGATTCAATAGAAGGTTGCCTTGATTCTGTTGAAGGACGTTTAGACTCTATGGAAAATCGCTTGGATTCAATGGAAACTCGCTTAGACAAAATCGATCCACCCAAATAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLIKIETTLAAQGEQIRIQGEQIRELQVEQKAQRQEFNARMDRLENLLVESIESTNKRFDSIEGRLDSMDSRLDSMENRLDSIEGCLDSVEGRLDSMENRLDSMETRLDKIDPPK", "aa_hexdigest": "ff28bdc9d3b467bde90dea85fcde23d3", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 90, "aa_cov": 0.6148148148148148, "hmm_cov": 0.9888888888888889, "evalue": 5.713800367420291e-32, "score": 105.82064819335938, "start": 2, "stop": 84 }, { "id": "PF23991.1", "name": "DUF7310", "length": 59, "aa_cov": 0.43703703703703706, "hmm_cov": 1.0, "evalue": 3.0951432054838176e-12, "score": 42.34527587890625, "start": 71, "stop": 129 } ], "seq_stats": { "molecular_weight": 15860.443099999991, "isoelectric_point": 4.626091575622559 }, "id": "CNOCLNGIAL_348", "locus": "GCA000143945_00313" }, { "type": "cds", "sequence": "contig_1", "start": 177564, "stop": 177887, "strand": "-", "frame": 1, "gene": null, "product": "DUF16 family-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6" ], "nt": "GTGAACACAGAATACGTAACGCGTAAAGAATTTAACGAGTACAAAGACTCAAACGACCAAAAACTTACAAAGATTGAAACTGAGTTAAAGAGTCAGGGTCAAAGGATTCAAGTTGTTGAGGACAAAGTTGATCGACTAACTGAAGTTGTTATGGTTCAGAGTCAACAAATCAAAACCCAGGGTGAAGCAATGAACGCCCGCATGGATCGCTTTGAAAGTCTTGTGCTTAAAAGTCTAGAATCAATCGGAAACACTTTGACTGATTTTGGTAAACGCCTCGACGTAATAGAAACTCGTTTAGACAAAATCGATCCGCCTAAATAA", "aa": "MNTEYVTRKEFNEYKDSNDQKLTKIETELKSQGQRIQVVEDKVDRLTEVVMVQSQQIKTQGEAMNARMDRFESLVLKSLESIGNTLTDFGKRLDVIETRLDKIDPPK", "aa_hexdigest": "0d246b975fb556e73b885e355c9d3f79", "start_type": "GTG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_A0AB38W8A6", "query_cov": 0.9906542056074766, "subject_cov": 0.954954954954955, "identity": 0.604, "score": 100.0, "evalue": 1.46e-25, "valid": false, "product": "DUF16 family-like protein", "uniref50_id": "UniRef50_A0AB38W8A6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6" ], "product": "DUF16 family-like protein" }, "genes": [], "id": "CNOCLNGIAL_349", "locus": "GCA000143945_00314" }, { "type": "cds", "sequence": "contig_1", "start": 178306, "stop": 178509, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGCCAAGTACACCGCAAGGGCTCTATTGCAGAAGCAGTTAGTGCCATCCAAGCTCATGATAAGATCGTGATCTTCCACCACATTCGTCCTGATGGCGATTGTTTGGGCGCACAACACGGCTTAGCGCGTTTAATCCAAACTAACTTTCCCCACAAGCAGGTCTTCTGTGTTGGTGATCCCAAACACAACTTTCCCTGA", "aa": "MNSQVHRKGSIAEAVSAIQAHDKIVIFHHIRPDGDCLGAQHGLARLIQTNFPHKQVFCVGDPKHNFP", "aa_hexdigest": "34d9eb2d6a77d3af142893ad558c069f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7434.424999999999, "isoelectric_point": 8.807944679260256 }, "id": "CNOCLNGIAL_350", "locus": "GCA000143945_00315" }, { "type": "cds", "sequence": "contig_1", "start": 178516, "stop": 178848, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGGTTTTCACTCCAAAGGAACAGATTACCCCGGAGTTAATGCAACAAGCCTTAGCCGTTATTGTTGATGCCAACTATAAGGAACGGATTGAGTGCCGGGACTTATTAGACCAAAACCAGTTTAAGGCAGTATTGCGGATTGACCACCACCCCAATGAGGACGATCTCAATACGACCCATAACTTCGTTGATGCGTCTTACATTGCCGCGGCTGAACAAGTGGTAGATCTAGCGGTGCAGGCCAAATGGAAGCTTAGCCCCCCAGCGGCTACGGCGCTGTATTTAGGTATTTATACAGATAGTAATAGGTTTCTATATAGTAATACATCATGA", "aa": "MVFTPKEQITPELMQQALAVIVDANYKERIECRDLLDQNQFKAVLRIDHHPNEDDLNTTHNFVDASYIAAAEQVVDLAVQAKWKLSPPAATALYLGIYTDSNRFLYSNTS", "aa_hexdigest": "8f0850adfe18df42c267cf5894436a9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 90, "aa_cov": 0.7454545454545455, "hmm_cov": 0.9555555555555556, "evalue": 6.020018464858525e-08, "score": 28.507266998291016, "start": 16, "stop": 97 } ], "seq_stats": { "molecular_weight": 12483.917699999985, "isoelectric_point": 4.811841392517089 }, "id": "CNOCLNGIAL_351", "locus": "GCA000143945_00316" }, { "type": "cds", "sequence": "contig_1", "start": 178870, "stop": 179280, "strand": "+", "frame": 1, "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.-.-", "GO:0003676", "GO:0004527", "GO:0008441", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_P75144", "UniRef:UniRef90_P75144" ], "nt": "ATGCTATATAGAGCTCAAGCTAATATAGCTAAGATCCATGATGAGTTAAACCACACTTCCTTAAAGGACATCCAGTTTAAACAATATGTCTTTAAAAACTTTCAGACCTTTCAGAATGTTATTTACTTTGTGGCCGATAAGAAGTTCCAAAAGAAATTAAAGGTAACACCCTTAGAATGTGCACGGGTAAATATCCTAGCTAACATTGAACAATTCCACATTTGGCTGTTCTTTATAGAAGAGGGTAAGAACCACTATCGGGTCGAATTCCGTAGTAACGGAATTAACGTACGCGAAGTAGCTTTAAAGTATGGTGGCGGGGGTCATATTCAGGCCAGCGGTGCAGTTCTTAAAAGCAAGCGCGACATAATTCGTGTAGTTCAAGATTGCCAAAAGCAAATTGCTGTATAA", "aa": "MLYRAQANIAKIHDELNHTSLKDIQFKQYVFKNFQTFQNVIYFVADKKFQKKLKVTPLECARVNILANIEQFHIWLFFIEEGKNHYRVEFRSNGINVREVALKYGGGGHIQASGAVLKSKRDIIRVVQDCQKQIAV", "aa_hexdigest": "6fdbde93fb658b294c76837d7d885a14", "start_type": "ATG", "rbs_motif": null, "genes": [ "nrnA" ], "truncated": "5-prime", "pseudogene": { "start": 178570, "stop": 179280, "observations": { "mutation": [ 178846 ], "stop": [ 178846 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75144", "query_cov": 1.0, "subject_cov": 0.41975308641975306, "identity": 1.0, "score": 276.0, "evalue": 2.68e-91, "gene_start": 1, "gene_end": 136, "reference_start": 189, "reference_end": 324, "reference_sequence": "MNSQVHRKGSIAEAVSAIQAHDKIVIFHHIRPDGDCLGAQHGLARLIQTNFPHKQVFCVGDPKHNFPWLEMVFTPKEQITPELMQQALAVIVDANYKERIECRDLLDQNQFKAVLRIDHHPNEDDLNTTHNFVDASYIAAAEQVVDLAVQAKWKLSPPAATALYLGIYTDSNRFLYSNTSWRTLYLGSMLYRAQANIAKIHDELNHTSLKDIQFKQYVFKNFQTFQNVIYFVADKKFQKKLKVTPLECARVNILANIEQFHIWLFFIEEGKNHYRVEFRSNGINVREVALKYGGGGHIQASGAVLKSKRDIIRVVQDCQKQIAV" }, "paralog": false, "identity": 0.9957627118644068, "score": 471.0, "evalue": 1.6e-170, "description": "Internal stop codon at 178846. Nonsense mutation around 178846.", "psc": { "uniref90_id": "UniRef90_P75144", "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75144", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "go_ids": [ "GO:0008441", "GO:0004527", "GO:0003676" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75144", "EC:3.1.-.-", "UniRef:UniRef50_P75144", "COG:COG0618", "COG:FT", "KEGG:K06881", "GO:0008441", "GO:0004527", "GO:0003676" ] }, "pscc": { "uniref50_id": "UniRef50_P75144", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75144" ], "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA" } }, "id": "CNOCLNGIAL_352", "locus": "GCA000143945_00317" }, { "type": "cds", "sequence": "contig_1", "start": 179293, "stop": 179499, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17E6AB", "UniRef:UniRef90_UPI001F17E6AB" ], "nt": "ATGCACCAAACCAAAAAAACTGCCTTGTCCAAGTCCACTTGGATTCTCATCCTCACCGCCACCGCCTCCCTCGCGACGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGA", "aa": "MHQTKKTALSKSTWILILTATASLATGLTVVGHFTSTTTTLKRQQFSYTRPDEVALRHTNAINPRLTP", "aa_hexdigest": "dd9509ba22f1693d7bc861382a14f493", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F17E6AB", "query_cov": 0.8823529411764706, "subject_cov": 0.8695652173913043, "identity": 0.983, "score": 114.0, "evalue": 3.08e-32, "valid": true, "uniref50_id": "UniRef50_UPI001F17E6AB" }, "pscc": { "uniref50_id": "UniRef50_UPI001F17E6AB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17E6AB" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7524.573299999999, "isoelectric_point": 11.0533784866333 }, "id": "CNOCLNGIAL_353", "locus": "GCA000143945_00318" }, { "type": "cds", "sequence": "contig_1", "start": 179566, "stop": 179823, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCGCAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNSAKGITAGSGSQQTTYDPTRTEAALTASTTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "e38d0c68fd329b9f24cab5f38593397d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 2.238700266952289e-09, "score": 32.50608444213867, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9090.854999999996, "isoelectric_point": 8.137860298156738 }, "id": "CNOCLNGIAL_354", "locus": "GCA000143945_00319" }, { "type": "cds", "sequence": "contig_1", "start": 179854, "stop": 180366, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_502", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571", "UniRef:UniRef90_P75285" ], "nt": "GTGGCGCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAGCAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSDSTNQTGAMFGLKVKDATVDSSKQSTESLKGEESSSSSTTSSTSTTQRGGSSNENKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTP", "aa_hexdigest": "9a176167c160360d61ab0198e2d19b7e", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "both", "pseudogene": { "start": 179566, "stop": 180669, "observations": { "mutation": [ 179821, 180364, 180526, 180541, 180565 ], "stop": [ 179821, 180364, 180526, 180541, 180565 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75285", "query_cov": 1.0, "subject_cov": 0.4028436018957346, "identity": 0.8, "score": 188.0, "evalue": 2.67e-55, "gene_start": 1, "gene_end": 170, "reference_start": 97, "reference_end": 265, "reference_sequence": "MRDNIAKGITAGSNTQQTTYDPTRTEATLTTATTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKDKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGYESKSSNGSSSTSTTQRGGSSNENKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTPWTPWLTTEQIHNDPAKFAASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFEQALLVFGICLS" }, "paralog": false, "identity": 0.8719346049046321, "score": 573.0, "evalue": 6.95e-209, "description": "Internal stop codon at 179821, 180364, 180526, 180541, 180565. Nonsense mutation around 179821, 180364, 180526, 180541, 180565.", "psc": { "uniref90_id": "UniRef90_P75285", "product": "Uncharacterized protein MPN_502", "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75285", "UniRef:UniRef50_P75571" ] }, "pscc": { "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571" ], "product": "Uncharacterized protein MPN_205" } }, "id": "CNOCLNGIAL_355", "locus": "GCA000143945_00320" }, { "type": "cds", "sequence": "contig_1", "start": 180583, "stop": 180927, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCTGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGTGGCAATGGCCATGTTACCAAGTCGGCCCACACCGCGCCTTTGAGTATAGGTGTCTTTAGGGTGCGCTATAATGCAACTGGTACCAGTGCTACTGTAACTGGTTGA", "aa": "MLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFGQALLVFGGNGHVTKSAHTAPLSIGVFRVRYNATGTSATVTG", "aa_hexdigest": "04e5edc98708378f800992127bd8d71b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 111, "aa_cov": 0.9736842105263158, "hmm_cov": 0.7657657657657657, "evalue": 1.0960963951145454e-32, "score": 109.00016021728516, "start": 4, "stop": 114 } ], "seq_stats": { "molecular_weight": 12343.444699999985, "isoelectric_point": 5.828349876403809 }, "id": "CNOCLNGIAL_356", "locus": "GCA000143945_00321" }, { "type": "cds", "sequence": "contig_1", "start": 180952, "stop": 181209, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "ATGGTCAACAAACAAACTGACGGGTTAAAGAATCTACCCTTTAACAATAACCGCTGGTTTGAATATGTACCACGGATGGCAGTTGCTGGCGCTAAGTTCGTTGGTAGGGAACTCGTTTTAGCGGGTACCATTACCATGGGTGATACCGCTACCGTACCTCGCTTACTGTACGATGAACTTGAAAGCAACCTGAACTTAGTAGCGCAAGGCCAAGGTCTTTTACGCGAAGACTTGCAACTCTTCACACCCTACGGATGA", "aa": "MVNKQTDGLKNLPFNNNRWFEYVPRMAVAGAKFVGRELVLAGTITMGDTATVPRLLYDELESNLNLVAQGQGLLREDLQLFTPYG", "aa_hexdigest": "5e7fb3bd21f7268e599038e7bfc5918b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 31, "aa_cov": 0.35294117647058826, "hmm_cov": 1.0, "evalue": 2.6825066785277696e-08, "score": 28.966754913330078, "start": 1, "stop": 30 } ], "seq_stats": { "molecular_weight": 9501.790699999994, "isoelectric_point": 5.212101554870604 }, "id": "CNOCLNGIAL_357", "locus": "GCA000143945_00322" }, { "type": "cds", "sequence": "contig_1", "start": 181501, "stop": 181644, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTGCTATTCTCAATCCGGAATTGTTAGCAGCTCTTCCCGACAAGGTTAAATACGGTAAGGAAAACGAGTTTGCTGCTAACGAGTACGAGCGCTTTAACCAGAAGTTAACGGTAGCTCCTACCCAAGGAACAAACTGA", "aa": "MFAAILNPELLAALPDKVKYGKENEFAANEYERFNQKLTVAPTQGTN", "aa_hexdigest": "d59c1f3728201f00d30247ea40cfac70", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5256.895600000003, "isoelectric_point": 5.1057558059692365 }, "id": "CNOCLNGIAL_358", "locus": "GCA000143945_00323" }, { "type": "cds", "sequence": "contig_1", "start": 181612, "stop": 181965, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAGGGTTCTTCGAGGCGAACCGCCTGTAATCATCGTCTGACTGCCCCCGAGGGTGGCTTGGACATTCGCTTTGAGGCCGACGCCGATGTTGGAAAACGTCGGGAGAGAGGAACGCGAACCACTTGTGTTCGTACTAATTCCGCTGGACGACCCCGCGCTGGTTTGAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTGCCATACCTGTACCCATTCCCAATCCAGGGCACATAATCCAACACCTGGTCGAGCACCGACCCCACAAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGGGATCAGTTTGTTCCTTGGGTAGGAGCTACCGTTAA", "aa": "MVEGSSRRTACNHRLTAPEGGLDIRFEADADVGKRRERGTRTTCVRTNSAGRPRAGLRRGYIIHAPVVIAIPVPIPNPGHIIQHLVEHRPHKVEPGGETGKRGGEVGSVCSLGRSYR", "aa_hexdigest": "187d4949352a696fdf6f23bd8641913b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12691.311699999991, "isoelectric_point": 10.722526741027831 }, "id": "CNOCLNGIAL_359", "locus": "GCA000143945_00324" }, { "type": "cds", "sequence": "contig_1", "start": 182062, "stop": 182235, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCCAGCAGGAACAATCAGGTACCTCCGCGGGGAATCCCGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGATGGATCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGA", "aa": "MGQQEQSGTSAGNPDSLKQDKISKSGDSLTTQDGNAMDQQEATNYTNLPPNLTPTAD", "aa_hexdigest": "c9c9d2561aa721679dd1b341c268daab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5998.275200000004, "isoelectric_point": 4.089076805114746 }, "id": "CNOCLNGIAL_360", "locus": "GCA000143945_00325" }, { "type": "cds", "sequence": "contig_1", "start": 182317, "stop": 182607, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNKSGQDDNSKFKAEDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRASGSGSNTTSSPGIGFKIPEQSGTNTTSKAVLITPGLA", "aa_hexdigest": "890f22afdbdbb58513e9f1ad165ff405", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10171.074699999988, "isoelectric_point": 5.170722770690916 }, "id": "CNOCLNGIAL_361", "locus": "GCA000143945_00326" }, { "type": "cds", "sequence": "contig_1", "start": 182830, "stop": 183870, "strand": "+", "frame": 1, "gene": "mgpA", "product": "adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009E1D434", "UniRef:UniRef90_UPI0009E1D434" ], "nt": "GTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAAGGGACGCCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTATCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCAGTAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAAAAAGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGATGTGGTGGGGGTTGGTCGACTTTCTGAAAGCAACGCCGCAAAGATGAACGACGATGTTGATGGTATTGTACGCACCCCACTCGCTGAACTGTTAGATGGGGAAGGACAAACAGCTGACACTGGTCCACAAAGCGTGAAGTTCAAGTCTCCTGACCAAATTGACTTCAACCGCTTGTTTACCCACCCAGTCACCGATCTGTTTGATCCGGTAACTATGTTGGTGTATGACCAGTACATACCGCTGTTTATTGATATCCCAGCAAGTGTGAACCCTAAAATGGTTCGTTTAAAGGTCTTGAGCTTTGACACCAACGAACAGAGCTTAGGTCTCCGCTTAGAGTTCTTTAAACCTGATCAAGATACCCAACCAAACAACAACGTTCAGGTCAATCCGAATAACGGTGACTTCTTACCACTGTTAACGGCCTCCAGTCAAGGTCCCCAAACCTTGTTTAGTCCGTTTAACCAGTGA", "aa": "MESVWDLKGVWADQAQSDSQGSTTTATRDALPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLVKPKKVIQSDKLDDDLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGTSSGNLSSVLSGGGAGGGSSGSGQSGVDLSPVEKVSGWLVGQLPSTSDGNTSSTNNLAPNTNTGNDVVGVGRLSESNAAKMNDDVDGIVRTPLAELLDGEGQTADTGPQSVKFKSPDQIDFNRLFTHPVTDLFDPVTMLVYDQYIPLFIDIPASVNPKMVRLKVLSFDTNEQSLGLRLEFFKPDQDTQPNNNVQVNPNNGDFLPLLTASSQGPQTLFSPFNQ", "aa_hexdigest": "3cae0f967659e255f457abfa67510bf9", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpA" ], "truncated": "both", "pseudogene": { "start": 182530, "stop": 184149, "observations": { "mutation": [ 182605, 182788, 183868 ], "stop": [ 182605, 182788, 183868 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0009E1D434", "query_cov": 1.0, "subject_cov": 0.5805369127516778, "identity": 0.991, "score": 655.0, "evalue": 4.21e-233, "gene_start": 1, "gene_end": 346, "reference_start": 142, "reference_end": 487, "reference_sequence": "SDQTKLNLPAYGEVNGLLNPALVETYFGNTRAGGSGSNTTSSPGIGFKIPEQNNDSKATLITPGLAWTPQDVGNLVVSGTTVSFQLGGWLVTFTDFVKPRAGYLGLQLSGLNASDSDQRELIWAPRPWAAFRGSWVNRLGRVESVWDLKGVWADQAQSDSQGSTTTATRNALPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLVKPKKVTQSDKLDDDLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGTSSGNLSSVLSGGGAGGGSSGSGQSGVDLSPVEKVSGWLVGQLPSTSDGNTSSTNNLAPNTNTGNDVVGVGRLSESNAAKMNDDVDGIVRTPLAELLDGEGQTADTGPQSVKFKSPDQIDFNRLFTHPVTDLFDPVTMLVYDQYIPLFIDIPASVNPKMVRLKVLSFDTNEQSLGLRLEFFKPDQDTQPNNNVQVNPNNGDFLPLLTASSQGPQTLFSPFNQWPDYVLPLAITVPIVVIVLSVTLGLAIGIPMHKNKQALKAGFALSNQKVDVLTKAVGSVFKEIINRTGISQAPKRLKQTSAAKPGAPRPPVPPKPGAPKPPVQPPKKPA" }, "paralog": false, "identity": 0.9647495361781077, "score": 1015.0, "evalue": 0.0, "description": "Internal stop codon at 182605, 182788, 183868. Nonsense mutation around 182605, 182788, 183868.", "psc": { "uniref90_id": "UniRef90_UPI0009E1D434", "gene": "mgpA", "product": "adhesin P1", "uniref50_id": "UniRef50_UPI0009E1D434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0009E1D434", "UniRef:UniRef50_UPI0009E1D434" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0009E1D434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009E1D434" ], "product": "adhesin P1" } }, "id": "CNOCLNGIAL_362", "locus": "GCA000143945_00327" }, { "type": "cds", "sequence": "contig_1", "start": 183880, "stop": 184197, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGCCGTTAGCGATCACTGTACCTATTGTTGTGATTGTGCTCAGTGTTACCTTAGGACTTGCCATTGGAATCCCAATGCACAAGAACAAACAGGCCTTGAAGGCTGGGTTTGCGCTATCAAACCAAAAGGTTGATGTGTTGACCAAAGCGGTTGGTAGTGTCTTTAAGGAAATCATTAACCGCACAGGTATCAGTCAAGCGCCAAAACGCTTGAAACAAACCAGTGCGGCTAAACCAGGAGCACCCCGCCCACCAGTACCACCAAAGCCAGGGGCTCCTAAGCCACCAGTGCAACCACCTAAAAAACCCGCTTAG", "aa": "MLPLAITVPIVVIVLSVTLGLAIGIPMHKNKQALKAGFALSNQKVDVLTKAVGSVFKEIINRTGISQAPKRLKQTSAAKPGAPRPPVPPKPGAPKPPVQPPKKPA", "aa_hexdigest": "73882c2df7ebb5c3c334b6e0860da49d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10932.12489999998, "isoelectric_point": 11.35315647125244 }, "id": "CNOCLNGIAL_363", "locus": "GCA000143945_00328" }, { "type": "cds", "sequence": "contig_1", "start": 184203, "stop": 184403, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCGAAGCTAAAGTTAAAACGTTATTTACTGTTTTTACCACTTTTACCGCTAGGGACGTTGTCACTAGCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGA", "aa": "MKSKLKLKRYLLFLPLLPLGTLSLANTYLLQDHNTLTPYTPFTTPLNGGLDVVRAAHLHPSYELVD", "aa_hexdigest": "305064b624de3835b370f99498e1956b", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7434.653600000002, "isoelectric_point": 9.157943534851075 }, "id": "CNOCLNGIAL_364", "locus": "GCA000143945_00329" }, { "type": "cds", "sequence": "contig_1", "start": 184410, "stop": 184976, "strand": "+", "frame": 3, "gene": null, "product": "Putative MgpC-like protein MPN_149", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037", "UniRef:UniRef90_P75037" ], "nt": "GTGGGGGATACCAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGATCAAAGTAATACCAACCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTATGTCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGATCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGA", "aa": "MGDTKLVALVRSALVRVKFQDTTSSDQSNTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYVLIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYKTLQDLLVEQPVTPYTPNAGLARVNGVAQDTVHFGSDQESSWNSQRSQKGLKNNPGPKAVTGFKLDKGRAYRKLNES", "aa_hexdigest": "21ee961df357f32fd42cbe48d8e4c8ff", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 184374, "stop": 185279, "observations": { "mutation": [ 184401, 184974, 185034 ], "stop": [ 184401, 184974, 185034 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75037", "query_cov": 1.0106382978723405, "subject_cov": 0.4377880184331797, "identity": 0.868, "score": 304.0, "evalue": 5.86e-100, "gene_start": 1, "gene_end": 188, "reference_start": 22, "reference_end": 210, "reference_sequence": "MAVGIFILSLNPSYELVDWKRVGDTKLVALVRSALVRVKFNDGTSSDSNNQDTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLELPYVKNESGAGDSTTTNSGSLYTTLQDLLVEQPVTPYTPNAGLARVNGAAQDTVHFGSGQESSWNSQRSQKVLKNNPGPKAVTGFKLDKGRAYRKLNEAWPVYEPLDSTKDGKGKDESSWNSSEKTTAESDAPLVGSTGSQMAAVTDSQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLETWAGENKYWNRYPCCWWCFSPSSDPAIVFHKWRSTECNHPVIPYFNRPTRLLKWPDRCDGFERGTEFVVLGRGWANHVRKGYLMSPHRVELGHRQAEGVCGESAWFQWNQRHRLA" }, "paralog": false, "identity": 0.8514851485148515, "score": 492.0, "evalue": 3.58e-176, "description": "Internal stop codon at 184401, 184974, 185034. Nonsense mutation around 184401, 184974, 185034.", "psc": { "uniref90_id": "UniRef90_P75037", "product": "Putative MgpC-like protein MPN_149", "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75037", "UniRef:UniRef50_P75037" ] }, "pscc": { "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037" ], "product": "Putative MgpC-like protein MPN_149" } }, "id": "CNOCLNGIAL_365", "locus": "GCA000143945_00330" }, { "type": "cds", "sequence": "contig_1", "start": 185079, "stop": 185306, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MSSTESQMAAVTDSQQSGHNSGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "01a905cfd228e3b6c2671a39b4f01a08", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7844.479600000001, "isoelectric_point": 5.985339164733887 }, "id": "CNOCLNGIAL_366", "locus": "GCA000143945_00331" }, { "type": "cds", "sequence": "contig_1", "start": 185565, "stop": 186242, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGAGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCACGGGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGA", "aa": "MNWGTDKQKQFVENQLGFKDDSNSSLTNFKSQGLTQPAYLIAGLDVVQDHLVFAAFKAGAVGYDMTTDSNASTKDQALAWSTTAGLDSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQKATAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLNEERLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVK", "aa_hexdigest": "76946e2353ae7cf95ac38c05c1c3a27c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.8711111111111111, "subject_cov": 0.8521739130434782, "identity": 0.934, "score": 349.0, "evalue": 3.65e-120, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_367", "locus": "GCA000143945_00332" }, { "type": "cds", "sequence": "contig_1", "start": 186270, "stop": 187658, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFF1225", "UniRef:UniRef90_UPI000AFF1225" ], "nt": "GTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGAAGGCGAGCGGAGCAGCGGAATGGGGTGAAGATTAGCACCTTGGAATCGCAAGCCACTGATGGCTTTGCCAACTCGTTACTTAACTTTGGTACCGGTCTTAAAGCCGGTGTTGACCCAGCTCCAGTAGCACGGGGTCATAAACCGAACTATAGTGCAGTACTACTAGTGCGTGGTGGCGTTGTAAGGTTAAACTTTAACCCCGATACTGATAAACTGTTGGATTCTACTGACAAAAACAGTGAACCTATCTCCTTCTCCTATACCCCATTTGGGTCTGCTGAAAGTGCCGTAGACCTCACCACGTTGAAGGATGTGACCTATATTGCTGAAAGTGGTCTGTGGTTCTATACCTTTGACAATGGTGAAAAACCAACGTACGATGGTAAACAACAACAGGTCAAAAACCGCAAGGGTTATGCTGTGATTACCGTATCACGTACCGGAATTGAATTTAACGAGGACGCTAATACCACAACCTTAAGCCAAGCCCCAGCTGCTTTGGCTGTCCAAAACGGGATTGCTTCCAGTCAGGACGACCTCACAGGCATCCTACCGTTATCCGATGAGTTCTCCGCTGTGATTACCAAGGATCAAACATGGACCGGTAAGGTTGATATCTATAAGAACACCAACGGGTTGTTTGAAAAGGATGATCAGCTATCGGAAAACGTGAAGAGGCGTGACAACGGTTTGGTCCCTATTTACAACGAAGGTATCGTCGATATTTGGGGCAGAGTGGATTTTGCTGCCAACAGTGTTTTGCAAGCGCGTAACCTCACTGATAAAACGGTTGATGAGGTGATCAATAACCCCGATATCCTCCAAAGCTTCTTTAAGTTTACCCCAGCCTTTGATAACCAAAGAGCAATGCTAGTGGGGGAAAAGACATCGGATACTACCTTAACGGTTAAACCGAAGATTGAGTACTTGGATGGTAACTTCTATGGTGAGGATTCCAAGATTGCTGGAATTCCGCTCAACATTGATTTCCCTTCCCGGATTTTTGCTGGCTTTGCTGCTTTACCGTCCTGGGTCATTCCGGTATCAGTCGGTTCATCGGTGGGCATTCTCTTAATCCTGCTCATCTTAGGCCTTGGTATTGGAATTCCAATGTATAAGGTCCGCAAGCTTCAAGACTCCAGCTTTGTTGATGTGTTTAAAAAGGTGGATACGTTGACAACCGCTGTGGGTAGCGTGTACAAGAAGATTATCACCCAAACGAGTGTGATCAAAAAAGCTCCTAGTGCGTTGAAAGCTGCTAATAACGCTGCTCCTAAAGCACCAGTTAAACCAGCTGCTCCAACAGCTCCAAGACCACCAGTCCAACCACCTAAAAAGGCTTAA", "aa": "MPYYYSANFPEAGNRRRAEQRNGVKISTLESQATDGFANSLLNFGTGLKAGVDPAPVARGHKPNYSAVLLVRGGVVRLNFNPDTDKLLDSTDKNSEPISFSYTPFGSAESAVDLTTLKDVTYIAESGLWFYTFDNGEKPTYDGKQQQVKNRKGYAVITVSRTGIEFNEDANTTTLSQAPAALAVQNGIASSQDDLTGILPLSDEFSAVITKDQTWTGKVDIYKNTNGLFEKDDQLSENVKRRDNGLVPIYNEGIVDIWGRVDFAANSVLQARNLTDKTVDEVINNPDILQSFFKFTPAFDNQRAMLVGEKTSDTTLTVKPKIEYLDGNFYGEDSKIAGIPLNIDFPSRIFAGFAALPSWVIPVSVGSSVGILLILLILGLGIGIPMYKVRKLQDSSFVDVFKKVDTLTTAVGSVYKKIITQTSVIKKAPSALKAANNAAPKAPVKPAAPTAPRPPVQPPKKA", "aa_hexdigest": "ee794a8dfd26ecf0921297c89b312275", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "5-prime", "pseudogene": { "start": 185970, "stop": 187658, "observations": { "mutation": [ 186240 ], "stop": [ 186240 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AFF1225", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.998, "score": 855.0, "evalue": 8.33e-309, "gene_start": 1, "gene_end": 462, "reference_start": 232, "reference_end": 693, "reference_sequence": "AFKAGAVGYDMTTDSSASTYNQALAWSTTAGLDSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQKATAKILNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLNEERLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVKWTNTASHYLPVPYYYSANFPEAGNRRRAEQRNGVKISTLESQATDGFANSLLNFGTGLKAGVDPAPVARGHKPNYSAVLLVRGGVVRLNFNPDTDKLLDSTDKNSEPISFSYTPFGSAESAVDLTTLKDVTYIAESGLWFYTFDNGEKPTYDGKQQQVKNRKGYAVITVSRTGIEFNEDANTTTLSQAPAALAVQNGIASSQDDLTGILPLSDEFSAVITKDQTWTGKVDIYKNTNGLFEKDDQLSENVKRRDNGLVPIYNEGIVDIWGRVDFAANSVLQARNLTDKTVDEVINNPDILQSFFKFTPAFDNQRAMLVGEKTSDTTLTVKPKIEYLDGNFYGEDSKIAGIPLNIDFPSRIFAGFAALPSWVIPVSVGSSVGILLILLILGLGIGIPMYKVRKLQDSSFVDVFKKVDTLTTAVGSVYKKIITQTSVIKKAPSALKAANNAAPKAPVKPAAPTAPRPPVQPPKKA" }, "paralog": false, "identity": 0.9166666666666666, "score": 1037.0, "evalue": 0.0, "description": "Internal stop codon at 186240. Nonsense mutation around 186240.", "psc": { "uniref90_id": "UniRef90_UPI000AFF1225", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI000AFF1225", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AFF1225", "UniRef:UniRef50_UPI000AFF1225" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AFF1225", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFF1225" ], "product": "MgpC family cytadherence protein" } }, "id": "CNOCLNGIAL_368", "locus": "GCA000143945_00333" }, { "type": "cds", "sequence": "contig_1", "start": 187775, "stop": 188056, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_143", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75143", "UniRef:UniRef90_P75143" ], "nt": "ATGAGTCATAAAGATTTTAATGGGCTTCAAGCCCCTCAGTTGTTAAGTTCGAGTTCCCCTGTTGCAAAGAAACAAAGTAGTCATAAACTCAGGCATGCCTTAAAGCACGCCCGTTACTTAAACCATAGTAGTAAGCGTACCCTCAAGCATGCCTTAGAGCTGCATGAAGACAACCAGGTCTTACTGGAAAAAGAAGGTAGTCCTAACTTCCAGGACTGGCTTTCTAAGCAACCTGGCGTCAATAAAACCAGCCTTAAGTACAACAAAAGCTTGGGTAGTTGA", "aa": "MSHKDFNGLQAPQLLSSSSPVAKKQSSHKLRHALKHARYLNHSSKRTLKHALELHEDNQVLLEKEGSPNFQDWLSKQPGVNKTSLKYNKSLGS", "aa_hexdigest": "6fb5f06c63730a34196b2433ee85fb01", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 187775, "stop": 188302, "observations": { "mutation": [ 188054 ], "stop": [ 188054 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75143", "query_cov": 1.0, "subject_cov": 0.5314285714285715, "identity": 1.0, "score": 179.0, "evalue": 3.88e-56, "gene_start": 1, "gene_end": 93, "reference_start": 1, "reference_end": 93, "reference_sequence": "MSHKDFNGLQAPQLLSSSSPVAKKQSSHKLRHALKHARYLNHSSKRTLKHALELHEDNQVLLEKEGSPNFQDWLSKQPGVNKTSLKYNKSLGSWISKESKPKKRFPPYFTYKGSKTTPEEAKALQQMKQSQKRFFHENMHSFLNEVAHNPMIQRFKQKQAKRAANTRQRTYKYRQ" }, "paralog": false, "identity": 0.9771428571428571, "score": 345.0, "evalue": 6.14e-124, "description": "Internal stop codon at 188054. Nonsense mutation around 188054.", "psc": { "uniref90_id": "UniRef90_P75143", "product": "Uncharacterized protein MPN_143", "uniref50_id": "UniRef50_P75143", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75143", "UniRef:UniRef50_P75143" ] }, "pscc": { "uniref50_id": "UniRef50_P75143", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75143" ], "product": "Uncharacterized protein MPN_143" } }, "id": "CNOCLNGIAL_369", "locus": "GCA000143945_00334" }, { "type": "cds", "sequence": "contig_1", "start": 188153, "stop": 188302, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAATCCCAAAAGCGCTTCTTTCACGAAAATATGCACAGCTTTCTCAATGAAGTTGCACATAACCCAATGATCCAGCGCTTTAATCAAAAGCAGGCCAAACGGGCTGCTAATACGCGACAACGCACTTACAAATACCGTCAGTAA", "aa": "MKQSQKRFFHENMHSFLNEVAHNPMIQRFNQKQAKRAANTRQRTYKYRQ", "aa_hexdigest": "97ec09b9e2f3be8f4311b6b0653e5851", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6124.919300000003, "isoelectric_point": 11.422459983825682 }, "id": "CNOCLNGIAL_370", "locus": "GCA000143945_00335" }, { "type": "cds", "sequence": "contig_1", "start": 188305, "stop": 188436, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W9U9", "UniRef:UniRef90_A0AB38W9U9" ], "nt": "ATGCACAAGATTAAACACTATAAACCCTTAAAGCGCAAGACCTGGTTAAACAAGGTTTCCAAGCAACGCCGCGAGCGCATGTCCGCTAAGTGCTATCAACCACCTAAGAGAGAAGTGATGCAGCCAACTTGA", "aa": "MHKIKHYKPLKRKTWLNKVSKQRRERMSAKCYQPPKREVMQPT", "aa_hexdigest": "7feeb616217eaab4132de434a7c43250", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W9U9", "query_cov": 1.0, "subject_cov": 0.9347826086956522, "identity": 1.0, "score": 86.7, "evalue": 8.35e-22, "valid": true, "uniref50_id": "UniRef50_A0AB38W9U9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W9U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W9U9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5366.414400000002, "isoelectric_point": 10.8630033493042 }, "id": "CNOCLNGIAL_371", "locus": "GCA000143945_00336" }, { "type": "cds", "sequence": "contig_1", "start": 188408, "stop": 188776, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAGGGTTCTTCAAGGCGAACCGCCCACAGTCGTCGTCTGACTGCCCCCGAGGGTGGCTTGGACATTCGCTTTGAGGCCAACGCCGATGTTGGAAAACGTCGGGAAAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCTGGTTTGAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTCCCATACCTGTACCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCCAGAACAGACCCCACAAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGGGATCAGGTGGTGCTAACTCTTTCAAGTTGGCTGCATCACTTCTCTCTTAG", "aa": "MVEGSSRRTAHSRRLTAPEGGLDIRFEANADVGKRREKGTRTTCVRTSSAGRPRAGLRRGYIIHAPVVIPIPVPIPNPGNIIQHLVQNRPHKVEPGGETGKRGGEVGSGGANSFKLAASLLS", "aa_hexdigest": "bf8e6c0e0c21f2e3291fed050395feef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12935.592499999986, "isoelectric_point": 11.435998344421389 }, "id": "CNOCLNGIAL_372", "locus": "GCA000143945_00337" }, { "type": "cds", "sequence": "contig_1", "start": 188876, "stop": 189049, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCAGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGACCGGTCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGA", "aa": "MGQQGQSGTSAGNPDSLKQDKISKSGDSLTTQDGNATGQQEATNYTNLPPNLTPTAD", "aa_hexdigest": "05af81cce8b055a65f0b9ea419c5cb62", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5838.084300000002, "isoelectric_point": 4.306201744079591 }, "id": "CNOCLNGIAL_373", "locus": "GCA000143945_00338" }, { "type": "cds", "sequence": "contig_1", "start": 189131, "stop": 189409, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDSNKFQATDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRAGGSGSNTTSSPGIGFKIPEQNNDSKATLITPGLA", "aa_hexdigest": "39714ed164769acd21b8843301f92ef5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9768.59219999999, "isoelectric_point": 5.086487388610839 }, "id": "CNOCLNGIAL_374", "locus": "GCA000143945_00339" }, { "type": "cds", "sequence": "contig_1", "start": 189632, "stop": 189937, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD7934", "PFAM:PF03257.18" ], "nt": "GTGGAGAGTGTGTGGGATTTAAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTGAAGCTTCCGGGTCAGCTTTGGCACCGCACCCGAATGCTTTGGCGTTTCAGATGAGTGTGGTGGAAGCGAGTGCTTACAGCTCTTCAACCTCAAGTTCGGGTTCCGGGTCAAGTTCAAACACCTCCCCCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCTAA", "aa": "MESVWDLKGVWADQAQLAAQAATSEASGSALAPHPNALAFQMSVVEASAYSSSTSSSGSGSSSNTSPYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLR", "aa_hexdigest": "78653c28708727d4ecb090a9f6664416", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034DD7934", "query_cov": 0.8415841584158416, "subject_cov": 0.9883720930232558, "identity": 0.588, "score": 87.0, "evalue": 1.71e-20, "valid": false, "uniref50_id": "UniRef50_UPI0034DD7934" }, "pscc": { "uniref50_id": "UniRef50_UPI0034DD7934", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD7934" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 56, "aa_cov": 0.5544554455445545, "hmm_cov": 1.0, "evalue": 8.254108365627868e-28, "score": 92.70142364501953, "start": 46, "stop": 101 } ], "seq_stats": { "molecular_weight": 10633.688699999986, "isoelectric_point": 6.7117414474487305 }, "id": "CNOCLNGIAL_375", "locus": "GCA000143945_00340" }, { "type": "cds", "sequence": "contig_1", "start": 190475, "stop": 191014, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_145", "db_xrefs": [ "RefSeq:WP_014325374.1", "SO:0001217", "UniParc:UPI0001DD64CE", "UniRef:UniRef100_A0AAX0SQH7", "UniRef:UniRef50_P75141", "UniRef:UniRef90_P75141" ], "nt": "ATGTCTTACTCACCTTCACTTAAGGAAATTATTGCTATTTTGCAAAAATACACTTCGAAAAACTACCAGTCAAATTGCAAAACTAGACCCGATGGTAAGCTTGAATTATCATTAAATGGAGTTTTTGAAGAAATCGTGAAAACACCCGGTAAAACTAGGTACGTAACCCATAAACAGCTTGATCAGAAATTAAAAGATTTCAAGCAAGATCTGATGGTTGAGTTGCACGACACGTTTGCTACTAAAACTGATCTTAAAGAATCGGAAGCACGCATTAACCAAAAGTTAGAAGCACTAATTCAAATTGTTATGGTTCAGGGTGAGCAGATAAAAGTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGAAAATTGAAGCTCAAGGACAACAAATTCAAAAAGTTAATGAAACTTTGAATTTTGTAGTTGAAAGCCTTGGATCTATCCACAAAAGACTCGATTCAATGGAAGGCCGCTTGGACTCAATGGAAAACCGTCTTGACAAACTAGAGTCCAAGTAG", "aa": "MSYSPSLKEIIAILQKYTSKNYQSNCKTRPDGKLELSLNGVFEEIVKTPGKTRYVTHKQLDQKLKDFKQDLMVELHDTFATKTDLKESEARINQKLEALIQIVMVQGEQIKVHGEQINKLTQTVEKQGEKIEAQGQQIQKVNETLNFVVESLGSIHKRLDSMEGRLDSMENRLDKLESK", "aa_hexdigest": "45147d98a56f0de92a7f04d6ba2c62a5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64CE", "ncbi_nrp_id": "WP_014325374.1", "uniref100_id": "UniRef100_A0AAX0SQH7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64CE", "RefSeq:WP_014325374.1", "UniRef:UniRef100_A0AAX0SQH7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQH7", "uniref90_id": "UniRef90_P75141" }, "psc": { "uniref90_id": "UniRef90_P75141", "product": "UPF0134 protein MPN_145", "uniref50_id": "UniRef50_P75141" }, "pscc": { "uniref50_id": "UniRef50_P75141", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75141" ], "product": "UPF0134 protein MPN_145" }, "genes": [], "id": "CNOCLNGIAL_376", "locus": "GCA000143945_00341" }, { "type": "cds", "sequence": "contig_1", "start": 191280, "stop": 191900, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_146", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140", "UniRef:UniRef90_P75140" ], "nt": "ATGCGATTTAAATATGGTGCAGTTGCTTTTAGTGGCTTTTTAGGTCTAGCAGTGATGTTGGCTAGTTGTGGTGCTAAAGGGCGTTTTGACCAGGTTGATGATGGCAAAATTAAGCTAGCTTCTTCACTTACCGGTAAGCGCGATGTGATACTGAAAGAAGTGCTCAACAAATATAACAGTCGCAAAGCCAAGGACGATTATCCGATTGAAATAACCAAAATAGCCGGTAGCTATGATGGTGGTCGATCTGATCTGCAAACGCGGTTGAGTGTCAAGGATAAAACAACCTTTTACAACATGATCTTGAATTATTCTGACACCATTTCTACTTTAGGTCGCAGTAACATGGAACTACCATTAGATAGCGTTGACGTATCACAGTTTTCAGAAAACTTCTTGAGCTTTAATGACCGCATAAGTGGTATTTCCAGAAAAGGCATTTACGGCATTCCGGTATCAATGTCAACTGATATCCTAGTAATCAACGGGCCGGTATTGCACTATATTTTGAATAGTGCAAAAAAGAAAGATGGTGCAGTAACTAAGAAAAACGCAAGTAATTCCAATGGTAACGAAGGAACGTTAACAGTAAATAACGATCAACAAACCACTGAACTATGA", "aa": "MRFKYGAVAFSGFLGLAVMLASCGAKGRFDQVDDGKIKLASSLTGKRDVILKEVLNKYNSRKAKDDYPIEITKIAGSYDGGRSDLQTRLSVKDKTTFYNMILNYSDTISTLGRSNMELPLDSVDVSQFSENFLSFNDRISGISRKGIYGIPVSMSTDILVINGPVLHYILNSAKKKDGAVTKKNASNSNGNEGTLTVNNDQQTTEL", "aa_hexdigest": "6386f4654261516e21c20b60a87aab8c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 191280, "stop": 192131, "observations": { "mutation": [ 191898, 192060 ], "stop": [ 191898, 192060 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75140", "query_cov": 1.0, "subject_cov": 0.7279151943462897, "identity": 1.0, "score": 324.0, "evalue": 1.19e-109, "gene_start": 1, "gene_end": 206, "reference_start": 1, "reference_end": 206, "reference_sequence": "MRFKYGAVAFSGFLGLAVMLASCGAKGRFDQVDDGKIKLASSLTGKRDVILKEVLNKYNSRKAKDDYPIEITKIAGSYDGGRSDLQTRLSVKDKTTFYNMILNYSDTISTLGRSNMELPLDSVDVSQFSENFLSFNDRISGISRKGIYGIPVSMSTDILVINGPVLHYILNSAKKKDGAVTKKNASNSNGNEGTLTVNNDQQTTELWKKIEEAAKTNGKTTQEQTKRDAKQSTSLIQLKEGSANTTEGNASESDKEIKKSWGNYQEVDGGLKGYTFKASVFEN" }, "paralog": false, "identity": 0.9929328621908127, "score": 479.0, "evalue": 4.75e-173, "description": "Internal stop codon at 191898, 192060. Nonsense mutation around 191898, 192060.", "psc": { "uniref90_id": "UniRef90_P75140", "product": "Uncharacterized protein MPN_146", "uniref50_id": "UniRef50_P75140", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75140", "UniRef:UniRef50_P75140" ] }, "pscc": { "uniref50_id": "UniRef50_P75140", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140" ], "product": "Uncharacterized protein MPN_146" } }, "id": "CNOCLNGIAL_377", "locus": "GCA000143945_00342" }, { "type": "cds", "sequence": "contig_1", "start": 192138, "stop": 193223, "strand": "+", "frame": 3, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75139", "UniRef:UniRef90_P75139" ], "nt": "ATGGTTGATTTTGCCACTAGGGTAGCGAAATCATTTAAGGATAAAGTTAGTGGTATCGATAATAAAAAAGGCACTGACATCCAAGGTGTTTTAGGTCTAGACAGTACACCAAACGTTTTGTTCGCTTCAGTATTTGCAGCTGGTGGTGGTAGCTATGACAACTTCTTTTACAAGGTAGAAAATGGCCGGGCTGATTTCCGTAATTTTGCAAATAAAGGTACTTCTTACAAAAACTTAGAGAAGGTTTACAACGATTACAAGAATTTAATCGGTAGCAACGGTCTTTTTGCTAGCAAAGATGGCTCTTACTCATCTAACTTTGAAAAGTTCCACCAGTTAGCTTTTTATGTAGGTTCTAGCTCTGGTTATAACTATGCTTTTGCTGATGAAACTGCTAAACGGTTAAAGTTTGGTGATAGTGCCATTGAGTATCCTAACGATACGCTTGAAATTAAGGTACCGACAAATAATAGTCAAAGTGGTGATGGGAATGGTGGCACCAATAACGACAATCTCTTAGGTACTTTTGACATTAGGGAAAAGAGTAACGGCAAAAAAGGTGAGAGCAACGGTAAACAAGGAAATGGTCAAGATAAAAAGACAATTTCGCTTTATAAAGATTCGATTCCTAAAGAAAAAACTGAAGGCACTGATGCAATTCTAATTAGCGATAATCAACTCATTAATCAATTACAAACTGCCGCTAAAACTAGTAGTTCACAAAATAAAGCTAACACTGCAGCTATAACTTTTAAGCAAAGCAATAAAAGTGAAACTGACCAAAGTACAACTAGTCAGTTTATCGGCTACACAACAACTGCTTCATTGAAAGCTGATAAGAAAAATATCTTTGATGTGAAGAAGCTAAATAACGAAAAGTCTGAGCGCAAAATCATTGTCGGTGCCACGGTGGAAACCTTAAATCAAGCAAACACATTGCAAGCTAATGAAGCCATTATTAAACCAGCACCTGGTAAGTACCAAAGTACTGATTCACACAAGGTCATGATTACTCAAGGCCCGAATATTGTCGGAATTCATGCCAACGAAAAAGAGGACAAAGAAACACAAAAGTTTATTAACTGA", "aa": "MVDFATRVAKSFKDKVSGIDNKKGTDIQGVLGLDSTPNVLFASVFAAGGGSYDNFFYKVENGRADFRNFANKGTSYKNLEKVYNDYKNLIGSNGLFASKDGSYSSNFEKFHQLAFYVGSSSGYNYAFADETAKRLKFGDSAIEYPNDTLEIKVPTNNSQSGDGNGGTNNDNLLGTFDIREKSNGKKGESNGKQGNGQDKKTISLYKDSIPKEKTEGTDAILISDNQLINQLQTAAKTSSSQNKANTAAITFKQSNKSETDQSTTSQFIGYTTTASLKADKKNIFDVKKLNNEKSERKIIVGATVETLNQANTLQANEAIIKPAPGKYQSTDSHKVMITQGPNIVGIHANEKEDKETQKFIN", "aa_hexdigest": "165fb00287905b6efd2736d312d96810", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 192138, "stop": 193526, "observations": { "mutation": [ 193221, 193245 ], "stop": [ 193221, 193245 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75139", "query_cov": 1.0, "subject_cov": 0.7443298969072165, "identity": 0.9940000000000001, "score": 673.0, "evalue": 7.22e-242, "gene_start": 1, "gene_end": 361, "reference_start": 1, "reference_end": 361, "reference_sequence": "MVDFATRVAKSFKDKVSGIDNKKGTDIQGVLGLDSTPNVLFASVFAAGGGSYDNFFYKVENGRADFRNFANKGTSYKNLEKVYNDYKNLIGSNGLFASKDGSYSSNFEKFHQLAFYVGSSSGYNYAFADETAKRLKFGDSAIEYPNDTLEIKAPTNNSQSGDGNGGTNNDNLLGTFDIREKSNGKKGESNGKQGNGQDKKTISLYKDSIPKEKTEGTDAILISDNQLINQLQTAAKTSSSQNKANTAAITFKQSNKSETDQSTTSQVIGYTTTASLKADKKNIFDVKKLNNEKSERKIIVGATVETLNQANTLQANEAIIKPAPGKYQSTDSHKVMITQGPNIVGIHANEKEDKETQKFINWYLNKEESWSVQNSGSTTTKKQTAAQYFAEQSSYITPLKNNFKADQSATKDSTVNTNYFTKQTFDLFKEVNDGKVLGFNDPSDFRSGKFRNTIGSTFNATISSKVDFNKFFENFKASLGSGFER" }, "paralog": false, "identity": 0.9913419913419913, "score": 892.0, "evalue": 0.0, "description": "Internal stop codon at 193221, 193245. Nonsense mutation around 193221, 193245.", "psc": { "uniref90_id": "UniRef90_P75139", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75139", "go_ids": [ "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75139", "UniRef:UniRef50_P75139", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75139", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75139" ], "product": "Uncharacterized protein MPN_147" } }, "id": "CNOCLNGIAL_378", "locus": "GCA000143945_00343" }, { "type": "cds", "sequence": "contig_1", "start": 193251, "stop": 193595, "strand": "+", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594" ], "nt": "GTGCAAAACAGCGGTTCAACTACAACTAAAAAGCAAACTGCTGCTCAATATTTTGCCGAACAGTCCTCTTACATCACTCCTTTAAAAAATAACTTTAAAGCAGATCAAAGTGCAACAAAAGATAGCACTGTTAACACTAACTATTTCACTAAACAAACATTTGATCTCTTCAAAGAAGTTAATGATGGAAAAGTGCTTGGTTTTAATGATCCAAGTGACTTTAGGAGTGGTAAGTTCCGCAATACTATTGGTTCTACTTTTAACGCAACCATCAGTTCTAAAGTTGACTTTAACAAGTTTTTTGAAAACTTTAAAGCTTCTTTAGGGTCTGGTTTTGAGCGTTAA", "aa": "MQNSGSTTTKKQTAAQYFAEQSSYITPLKNNFKADQSATKDSTVNTNYFTKQTFDLFKEVNDGKVLGFNDPSDFRSGKFRNTIGSTFNATISSKVDFNKFFENFKASLGSGFER", "aa_hexdigest": "d4ca02fa10a084a1bd117b29b5139ba6", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XG79", "query_cov": 0.9385964912280702, "subject_cov": 0.8492063492063492, "identity": 0.523, "score": 110.0, "evalue": 4.69e-29, "valid": false, "product": "Lipoprotein", "uniref50_id": "UniRef50_P75594" }, "pscc": { "uniref50_id": "UniRef50_P75594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594" ], "product": "Uncharacterized lipoprotein MPN_098" }, "genes": [], "id": "CNOCLNGIAL_379", "locus": "GCA000143945_00344" }, { "type": "cds", "sequence": "contig_1", "start": 193760, "stop": 194044, "strand": "+", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGCACAAAAAGAAGTTTTTATCAAGAACTTCCTTTGCTTCTTTGTTTTTGCTTTTAGGAACAGTATTGAGTGCTTGTAGCAAAGTAGAGTCCACAAAAAGTGTTCATCCTGTAAAAAGTTTTAAATCTGATCTAAAAAGTCTTCTTAAAGAAACAATTAAACAAGATATTGATTTATCTAAAACAATCAAAACTTCAGAAGGCAAACAACAGATAATAACTTCTTTAAAAAAGTCTTACGGAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCATGA", "aa": "MHKKKFLSRTSFASLFLLLGTVLSACSKVESTKSVHPVKSFKSDLKSLLKETIKQDIDLSKTIKTSEGKQQIITSLKKSYGVNPKDTTKLLLDA", "aa_hexdigest": "fa1f03d69dc5fd69fe4f6f3ed7b80375", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.9306930693069307, "identity": 0.606, "score": 100.0, "evalue": 9.49e-26, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "CNOCLNGIAL_380", "locus": "GCA000143945_00345" }, { "type": "cds", "sequence": "contig_1", "start": 194129, "stop": 194218, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGGTTGATCATTTCCAAATGACATACCAAAGTTTTAAGGATTTATCAATTAAAGCTAAGTTGAGCTATACCTTTAATTGA", "aa": "MERKVDHFQMTYQSFKDLSIKAKLSYTFN", "aa_hexdigest": "893f67d2558641e21e4c66ec2ae765db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3557.0605000000005, "isoelectric_point": 9.302803993225098 }, "id": "CNOCLNGIAL_381", "locus": "GCA000143945_00346" }, { "type": "cds", "sequence": "contig_1", "start": 194544, "stop": 194690, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64D2", "UniRef:UniRef100_A0AB38W7S6", "UniRef:UniRef50_A0AB38W7S6", "UniRef:UniRef90_A0AB38W7S6" ], "nt": "ATGAGCTTTTGGAAGTGTCAACAAAATTGCAAAAAAGTTTTTGATGAAATTAATTTGAATAAAAATAAAAAGCCATCTGTTCAAGGCGAAAAGCAAGTGGCTAAAATTTTTCCAAGTACAACCAAGCAATCACAACAAATTACCTAA", "aa": "MSFWKCQQNCKKVFDEINLNKNKKPSVQGEKQVAKIFPSTTKQSQQIT", "aa_hexdigest": "dc6a5950de81f626c12ae291ce84ec9f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64D2", "uniref100_id": "UniRef100_A0AB38W7S6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64D2", "UniRef:UniRef100_A0AB38W7S6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7S6", "uniref90_id": "UniRef90_A0AB38W7S6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W7S6", "uniref50_id": "UniRef50_A0AB38W7S6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7S6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7S6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5587.413200000003, "isoelectric_point": 9.7619478225708 }, "id": "CNOCLNGIAL_382", "locus": "GCA000143945_00347" }, { "type": "cds", "sequence": "contig_1", "start": 194871, "stop": 195077, "strand": "+", "frame": 3, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4", "UniRef:UniRef90_A0AAP8E4D4" ], "nt": "ATGATAGAAGTATTAAGTGATTCTGAAATTTTATCTTATTTATTAAGTAATAAATACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAG", "aa": "MIEVLSDSEILSYLLSNKYTADGVNEIYVTMNIKDKDSNTQRKPDKMSLELELFLNQFIEFLKEQRKS", "aa_hexdigest": "1c2b98a315da9777c8492c7fded3d0bd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAP8E4D4", "query_cov": 1.0, "subject_cov": 0.8095238095238095, "identity": 1.0, "score": 130.0, "evalue": 3.71e-38, "valid": true, "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D4" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4" ], "product": "Family K-like protein" }, "genes": [], "id": "CNOCLNGIAL_383", "locus": "GCA000143945_00348" }, { "type": "cds", "sequence": "contig_1", "start": 195167, "stop": 195736, "strand": "+", "frame": 2, "gene": null, "product": "Putative MgpC-like protein MPN_149", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037", "UniRef:UniRef90_P75037" ], "nt": "GTGGGTGATACCAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTAATGATGGGACGAGTTCGGATTCTAATAACCAAGATACGAATCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTAGAGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGCTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGTCCTTAAAAACAACCCCGGACCCAAAGTCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTAAATGAAGCTTGA", "aa": "MGDTKLVALVRSALVRVKFNDGTSSDSNNQDTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLELPYVKNESGAGDSTTTNSGSLYTTLQDLLVEQPVTPYTPNAGLARVNGAAQDTVHFGSGQESSWNSQRSQKVLKNNPGPKVVTGFKLDKGRAYRKLNEA", "aa_hexdigest": "ceca23a010009fdea17d89071a801007", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 195104, "stop": 196039, "observations": { "mutation": [ 195158, 195734 ], "stop": [ 195158, 195734 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75037", "query_cov": 1.0, "subject_cov": 0.43548387096774194, "identity": 0.9890000000000001, "score": 357.0, "evalue": 5.43e-121, "gene_start": 1, "gene_end": 189, "reference_start": 22, "reference_end": 210, "reference_sequence": "MAVGIFILSLNPSYELVDWKRVGDTKLVALVRSALVRVKFNDGTSSDSNNQDTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLELPYVKNESGAGDSTTTNSGSLYTTLQDLLVEQPVTPYTPNAGLARVNGAAQDTVHFGSGQESSWNSQRSQKVLKNNPGPKAVTGFKLDKGRAYRKLNEAWPVYEPLDSTKDGKGKDESSWNSSEKTTAESDAPLVGSTGSQMAAVTDSQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLETWAGENKYWNRYPCCWWCFSPSSDPAIVFHKWRSTECNHPVIPYFNRPTRLLKWPDRCDGFERGTEFVVLGRGWANHVRKGYLMSPHRVELGHRQAEGVCGESAWFQWNQRHRLA" }, "paralog": false, "identity": 0.9903536977491961, "score": 603.0, "evalue": 6.18e-220, "description": "Internal stop codon at 195158, 195734. Nonsense mutation around 195158, 195734.", "psc": { "uniref90_id": "UniRef90_P75037", "product": "Putative MgpC-like protein MPN_149", "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75037", "UniRef:UniRef50_P75037" ] }, "pscc": { "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037" ], "product": "Putative MgpC-like protein MPN_149" } }, "id": "CNOCLNGIAL_384", "locus": "GCA000143945_00349" }, { "type": "cds", "sequence": "contig_1", "start": 195740, "stop": 196156, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAACCCCTGGATTCGACCAAGGATGGCAAGGGGAAGGATGAGAGCTCTTGGAACAGTTCGGAAAAAACAACGGCGGAAAGTGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATACCTCAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGGGCGGGGGAAAACAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCCACAAATGA", "aa": "MYEPLDSTKDGKGKDESSWNSSEKTTAESDAPLVGSTGSQMAAVTDSQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLETWAGENKYWNRYPCCWWCFSPSSDPAIVFHK", "aa_hexdigest": "1088188cd4fd0a9a1f87456e227f457c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14993.192999999997, "isoelectric_point": 5.013051414489746 }, "id": "CNOCLNGIAL_385", "locus": "GCA000143945_00350" }, { "type": "cds", "sequence": "contig_1", "start": 196326, "stop": 196877, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W870", "UniRef:UniRef90_A0AB38W870" ], "nt": "TTGAACTGGGGCACCGACAAGCAGAAGGAGTTTGTGGAGAATCAGCTTGGTTTCAATGAAACCAGCGCCACCGACTCGCATAACTTTAAAAAAGCGCTGTTACATCAACCCGCCTACCTCATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTCGAGCCGGCGCGGTGGGGTATGATATGCGACTGATACGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAACGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACGACTTATCCGGTCAAGTCCGACCAAAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATAAAAAAAGCCATCCACCTAAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACGATTGA", "aa": "MNWGTDKQKEFVENQLGFNETSATDSHNFKKALLHQPAYLISGLDVVADHLVFAAFRAGAVGYDMRLIRTLRPTTKRSPGRPRPGWTVLGGKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSKNTEAVQTTYPVKSDQKASAKIASLINASPLNSYGDKKSHPPKVKGRQLQNIFDD", "aa_hexdigest": "2d9d500635600d3efd1bae932f475baa", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W870", "query_cov": 0.9398907103825137, "subject_cov": 0.945054945054945, "identity": 0.9940000000000001, "score": 343.0, "evalue": 2.41e-119, "valid": true, "product": "MgpC protein", "uniref50_id": "UniRef50_A0AB38W870" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W870", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W870" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_386", "locus": "GCA000143945_00351" }, { "type": "cds", "sequence": "contig_1", "start": 197309, "stop": 197791, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_151", "db_xrefs": [ "RefSeq:WP_014574897.1", "SO:0001217", "UniParc:UPI0001DD64D4", "UniRef:UniRef100_A0AAX0SNI3", "UniRef:UniRef50_P75490", "UniRef:UniRef90_P75035" ], "nt": "TTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGAATTTAATGGCAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAACGAATATAAAGACTCAAACGACCAACGGCTCATAAAGATTGAAACTACTTTGGCTGCCCAAGGTGAGCAAATTAGTCAGCTAGTTCAAATTGTGTTCCTCCAAGGAAAACAAATTAAAGAACTTCAAGCGGAGCAAAAAGCACAAAGAGTTGAATTTAACGCGCGCATGGATCGCTTTGAAAGCCTTGTGCTTAAGAGTCTGGAATCAATCGGAAACACTTTGACTGATTTTGGTAAACGACTCGATTCAATGGAAACTCGTTTGGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTAGACAAAATCGATCCACCTAAATAG", "aa": "MFKKRLNKDKINNCHVWEEELPDGSYDMEFNGNLNHMEKRKSGYVTQKQFNEYKDSNDQRLIKIETTLAAQGEQISQLVQIVFLQGKQIKELQAEQKAQRVEFNARMDRFESLVLKSLESIGNTLTDFGKRLDSMETRLDSMDGRLDSMETRLDKIDPPK", "aa_hexdigest": "1e7763080aa0ad97420f12daf9c242b6", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64D4", "ncbi_nrp_id": "WP_014574897.1", "uniref100_id": "UniRef100_A0AAX0SNI3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64D4", "RefSeq:WP_014574897.1", "UniRef:UniRef100_A0AAX0SNI3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SNI3", "uniref90_id": "UniRef90_P75035" }, "psc": { "uniref90_id": "UniRef90_P75035", "product": "UPF0134 protein MPN_151", "uniref50_id": "UniRef50_P75490" }, "pscc": { "uniref50_id": "UniRef50_P75490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75490" ], "product": "UPF0134 protein MPN_287" }, "genes": [], "id": "CNOCLNGIAL_387", "locus": "GCA000143945_00352" }, { "type": "cds", "sequence": "contig_1", "start": 198073, "stop": 198723, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTAAGTACGGCGCTATTGTTTTTAGTTGTCTTTTGGGCGTTTCGGCCATTTTAGCTGCCTGTGGGGCAAGGGGCAAATTTAACCAAGTTGATGATGGCAAGATTAAGCTAGCTTCATCTTTAACTTCCAGAAGTGCGTCAACAGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGCGTAAATGATTACCCCATTGAAATTACCCAAATTGCTGGCGGTTATGATGGCGGGCGGACTGATCTACAAACCCGAGTTAACGTTAAAGACACGACTAATTTTTATAACCTCATCTTGAACTATCCCGACTTAGTTTCCACTTTGGGACGTGTTGGGATGGAACTACCTTTTGATAAGGTTAAAACTGATAAGTTAGCTGACCGTTTTTTGGATTTCAACAACCGTATTAGTGCCATTTCCAAAAAGGGCATTTATGGCATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATTAATGGACCCGTTTTGCACTATATTTTGAATAGTGCACAAGGAAAGGTTAGTTCTGTCACTTCGGTGCAAAGAACAGCAGCTTCTGGTAGTGGAACAACAAATAATAATGGAGTAACAAAACCTTTGAAGATTGATAAAGAAAATGAATCTACAAAGAAACTTTGA", "aa": "MKFKYGAIVFSCLLGVSAILAACGARGKFNQVDDGKIKLASSLTSRSASTALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELPFDKVKTDKLADRFLDFNNRISAISKKGIYGIPVSLSTEVLSINGPVLHYILNSAQGKVSSVTSVQRTAASGSGTTNNNGVTKPLKIDKENESTKKL", "aa_hexdigest": "ea40edbbeed660d617dde646146b80b4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23288.397800000013, "isoelectric_point": 9.607932853698731 }, "id": "CNOCLNGIAL_388", "locus": "GCA000143945_00353" }, { "type": "cds", "sequence": "contig_1", "start": 198808, "stop": 198951, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCACTCAAACTCAACCTACTGACAATAGTAACGATGCTAATCAAAGCGACCAGGCGATAGAAAAGACTTGGGGTAAATATCAGGAGGTTGAAGGTGGTTTAAAAGATTACACCTTCAAAGCCAGTGTGTTTGAAAACTGA", "aa": "MSTQTQPTDNSNDANQSDQAIEKTWGKYQEVEGGLKDYTFKASVFEN", "aa_hexdigest": "20fa1cb098c572dd2efe284f89084f46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5302.5751, "isoelectric_point": 4.285114479064942 }, "id": "CNOCLNGIAL_389", "locus": "GCA000143945_00354" }, { "type": "cds", "sequence": "contig_1", "start": 198958, "stop": 200154, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75034" ], "nt": "TTGCTTGACTTTTCTAATAGGGTAGCTAAGTCATTTAGCAATATAAACACTAACACCAATAAAAAGGGCACCGATATTCAAGGTGTTCTGGGAATCGATAGCACTCCTAACTCCTTATTTACCTCTGTTTTTGCTGCAGGTGGCGGTAACTATGACAACTTCTTTTATAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCTTACCAAAACTTACAAAAAGTTTATGGTGATTACAAAGGCTTGATTGACAGCAATGGTCTCTTTGTTAATAAGGGTGGTTCTTATTCATCGAACTTCCAAAAGTTTCACCAATTAGCTTACAGTATTTCCTCGACTTCCGGCTTCTTCTACTCGTTTGCTGGTGAAAACTCAAAGCGCTTGAAGTTTGATGGCGGTAATTTTATTGAGTTTCCTGGATTTACTCAAGCAATTTATGCGCCTGAAAATAATAGTCAAAGTGGTGGAGAGAGTGGCGATCAAACCACAAATAATGAAGCCAATCTTTTAGGCACTTTCAAAATAAAAAGCTCTAATAAATCTAAATCTTCAAGTTCCAAATCAAGCACTAAAGCCGAAACAGGCAAAACTAGTGGTGGAGACCAAAACCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAACGGAGGTGAAGAAACAACTATTTACCTTTATAAAAACTCAATTCCATCTGGAAAAAATAAAGGCGAAAATGCAATTCTTATAGATAACAAAACTATTGTGGAACAACTAGAAAGTGCGGCTAAAAAAGAAGATAAGAAGAGTGGCGAATCTACCACAGAACAGACACAAATACAAAGTAAAAGTGTAACAGAACAAAAACAACCCAAAATCATCGGTTACACTACCACTGGCAATGTTCACGAAGACAACAAACACATTTTCCCAATTGACAAACTTACATCTGATCGAAATTTTGACCGCAAAATTATTGTTGGTGCCACTGAAGAAACACTCGATAAATCGAATACTTTGCAGTCCAATGAAGCCATTGTTTTACCAGCACCCTCTAAGTACAAAAGTACTGACACTAACAAAGTAACCATCACCCAAGGTCCGAATATCATTGGAATTCACGCCAACGAAAAGGAAAATGCAGAAACACAAAAGTTTGTGGATTGA", "aa": "MLDFSNRVAKSFSNINTNTNKKGTDIQGVLGIDSTPNSLFTSVFAAGGGNYDNFFYKVANGRADFSNFKNKGTSYQNLQKVYGDYKGLIDSNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGENSKRLKFDGGNFIEFPGFTQAIYAPENNSQSGGESGDQTTNNEANLLGTFKIKSSNKSKSSSSKSSTKAETGKTSGGDQNQGKKGEGAQNQGKKGEGAQNQGKNGGEETTIYLYKNSIPSGKNKGENAILIDNKTIVEQLESAAKKEDKKSGESTTEQTQIQSKSVTEQKQPKIIGYTTTGNVHEDNKHIFPIDKLTSDRNFDRKIIVGATEETLDKSNTLQSNEAIVLPAPSKYKSTDTNKVTITQGPNIIGIHANEKENAETQKFVD", "aa_hexdigest": "0eab26311ca660acbccde69ba9c1b775", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 198658, "stop": 200457, "observations": { "mutation": [ 198721, 198949, 200152, 200176 ], "stop": [ 198721, 198949, 200152, 200176 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75034", "query_cov": 1.0, "subject_cov": 0.4830097087378641, "identity": 0.997, "score": 697.0, "evalue": 1.61e-245, "gene_start": 1, "gene_end": 398, "reference_start": 296, "reference_end": 693, "reference_sequence": "MKFKYGAIVFSCLLGVSAILAACGARGKFNQVDDGKIKLASSLTSRSASTALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELPFDKVKTDKLADRFLDFNNRISAISKKGIYGIPVSLSTEVLSINGPVLHYILNSAQGKVSSVTSVQRTAASGSGTTNNNGVTKPLKIDKENESTKKLWEEIENKAKENGKKTSSSRRKRNLSSSKQMSTQTQPTDNSNDANQSDQAIEKTWGKYQEVEGGLKDYTFKASVFENWHDLLDFSNRVAKSFSNINTNTNKKGTDIQGVLGIDSTPNSLFTSVFAAGGGNYDNFFYKVANGRADFSNFKNKGTSYQNLQKVYGDYKGLIDSNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGENSKRLKFDGGNFIEFPGFTQAIYAPENNSQSGGESGDQTTNNEANLLGTFKIKSSNKSKSSSSKSSTKAETGKTSGGDQNQGKKGEGAQNQGKKGEGAQNQGKNGGEETTIYLYKNSIPSGKNKGENAILIDNKTIVEQLESAAKKEDKKSGESTTEQTQIQSKSVTEQKQPKIIGYTTTGNVHEDNKHIFPIDKLTSDRNFDRKIIVGATEETLDKSNTLQSNEAIVLPAPSKYKSTDTNKVTITQGPNIIGIHANEKENAETQKFVDWFLNSPQTWEGKGKGKEQTNKTAAEFFAESASYILPLKEIFEQKNEKKEGSDQKDSKSNGRGKQTNLYTEKALELFRGISTDQIVSYSDPSDFRSGRFRDGIGATFNAAVSSKASFEKFVQNFIATLGSDI" }, "paralog": false, "identity": 0.993322203672788, "score": 1114.0, "evalue": 0.0, "description": "Internal stop codon at 198721, 198949, 200152, 200176. Nonsense mutation around 198721, 198949, 200152, 200176.", "psc": { "uniref90_id": "UniRef90_P75034", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75034", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "CNOCLNGIAL_390", "locus": "GCA000143945_00355" }, { "type": "cds", "sequence": "contig_1", "start": 200254, "stop": 200547, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCACTCAAGGAAATTTTTGAGCAAAAGAATGAAAAGAAAGAAGGCAGCGATCAAAAAGATAGTAAAAGCAATGGTAGAGGTAAACAAACTAACCTCTACACCGAAAAGGCCTTGGAACTATTTAGAGGTATTAGTACCGATCAAATAGTAAGTTACAGTGACCCAAGTGACTTTAGGAGTGGTCGTTTCCGTGATGGAATAGGTGCCACTTTTAACGCAGCAGTAAGCTCTAAAGCATCTTTTGAAAAGTTTGTTCAAAACTTTATTGCAACTTTAGGTAGTGATATTTAG", "aa": "MPLKEIFEQKNEKKEGSDQKDSKSNGRGKQTNLYTEKALELFRGISTDQIVSYSDPSDFRSGRFRDGIGATFNAAVSSKASFEKFVQNFIATLGSDI", "aa_hexdigest": "7e6d7d2e5f9ef87dd24074610f5d1eac", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10805.850299999987, "isoelectric_point": 7.944713020324707 }, "id": "CNOCLNGIAL_391", "locus": "GCA000143945_00356" }, { "type": "cds", "sequence": "contig_1", "start": 200868, "stop": 201086, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGCATTGAAGGTAATGTTTACAAACACTTCCAAACTGAACTTAGTCGCTTTTACAAGCAAACGCGCAAACACTTTCGCGAAACTGGTTCGGAAAGTCTCTTTTTAGGTTTACCAGTAATTGAGGGCATTAACGAGTTTAACGATGTCTTTCGTGCTCCTTTACTCTATGTGGGGGTTAAGTTAAAAGTAGCACCACGACTTGAACGCTTTTGA", "aa": "MFGIEGNVYKHFQTELSRFYKQTRKHFRETGSESLFLGLPVIEGINEFNDVFRAPLLYVGVKLKVAPRLERF", "aa_hexdigest": "d192c8883fe593f4effd274614fb1862", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8465.699599999993, "isoelectric_point": 9.518192863464353 }, "id": "CNOCLNGIAL_392", "locus": "GCA000143945_00357" }, { "type": "cds", "sequence": "contig_1", "start": 201087, "stop": 202010, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGGAAATAAACCGCGAGGAAATCTTTTTAAATCCGACCATCATCGGGGTTGAAATTAACAAGCGTAACAGCTTGTTTAAAAATAACTATGACACCACTAAGGTTGATATTAACCAGGCATTGGAAATCTTTCGTGAGTTGGAATACCAATTTCGGATGCCACTCACCTCGGAGTTAAAAAGTTTTTCCAAAAAGGCTAAGAGTGACTTTAACACTGAAAAACGGACTAACTTCCTCACTAATAACGTTCTTTTAGGAATCTTTGACGTTAAGGGGGATCAACTGTTCCAAAACTTTAACGAAATTCTAAACACTGATCCTGATGTTTTGGATGAATTGTTAAAGGATCGCCGCGATCTCTTATACGACAACCGTGAGTTTCGTGAAAACTTTAACTTAAAGGGCACTTACCTCTTTAGCCACTTAGACATCTTCCAGCAGTACGCTGTCAAACAGGCATTTGATGGGGATGTCATTATTGAAGGTCCACCTGGTACTGGTAAGAGTGAAACGATTGTCAACATCTTGGTCAACCTCGCTTTAAACAAAAAGAAGGTGTTGTTTGTGTCAGAAAAGGTAACTGCCTTAGATGTCGTTTACAACCGGCTAGGTAGCTTTAAGCACATTGCCTTATTTAATGCTTCGGTAGCGAGTGAGAAGAAGCGCTTTTACAGCCAGTTCGCTGATTATGAAAGCTTCTTTACCGATAACTTTTCCAAGAAGGATTTAGTTAACGAAATGCCGGTGTTTGACGGGCAGTGGGTTGATAAGATCCTAAGTGAATTCACTAACCTTCAAAACATTTACGATACCCAAATTAATTCGGGTAACCAGAGCTATTCCTTCAAGGAAATCCTAAGTAGTTTTCCAATACTTGATGTCAGTTACATTAAGATCAAGGAACACGATCGTTTTGATGAATGA", "aa": "MEINREEIFLNPTIIGVEINKRNSLFKNNYDTTKVDINQALEIFRELEYQFRMPLTSELKSFSKKAKSDFNTEKRTNFLTNNVLLGIFDVKGDQLFQNFNEILNTDPDVLDELLKDRRDLLYDNREFRENFNLKGTYLFSHLDIFQQYAVKQAFDGDVIIEGPPGTGKSETIVNILVNLALNKKKVLFVSEKVTALDVVYNRLGSFKHIALFNASVASEKKRFYSQFADYESFFTDNFSKKDLVNEMPVFDGQWVDKILSEFTNLQNIYDTQINSGNQSYSFKEILSSFPILDVSYIKIKEHDRFDE", "aa_hexdigest": "aacb909093bef0fa201f8ade522348c4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 73, "aa_cov": 0.20846905537459284, "hmm_cov": 1.0, "evalue": 1.3046236514158374e-09, "score": 33.647674560546875, "start": 142, "stop": 205 } ], "seq_stats": { "molecular_weight": 35840.027000000024, "isoelectric_point": 5.108881950378417 }, "id": "CNOCLNGIAL_393", "locus": "GCA000143945_00358" }, { "type": "cds", "sequence": "contig_1", "start": 202152, "stop": 202385, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTGCTATGTGTTGGATTACTTCGAGCAGAACAACAGCATAATCAAACCGAAGCGGGTATTGCTCTATACCCCCACTGAACGCGGGCAAAAGCAGTTGCACCAACTCCAGCAAGACGTTGCTAAGTACAACAGCTTGCAACGTTTTAAATCGGCTGCTAAGTTTGAAACAATTAAGCTCAATTTAGCTAACAAACTAGCACAAAACGCCAAGCCTTTTTTCTTTTCTTGA", "aa": "MLCYVLDYFEQNNSIIKPKRVLLYTPTERGQKQLHQLQQDVAKYNSLQRFKSAAKFETIKLNLANKLAQNAKPFFFS", "aa_hexdigest": "b38c3cda4b3cce2177ce1bfc3b606855", "start_type": "GTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9069.429899999994, "isoelectric_point": 9.812168693542482 }, "id": "CNOCLNGIAL_394", "locus": "GCA000143945_00359" }, { "type": "cds", "sequence": "contig_1", "start": 202413, "stop": 203105, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGGAAAATCTCGTACAAACCCAAAAGCAATTGGTTAAGGCTAAGCAAAGCTACTTAAGCAAAATTGAGCAGTATGTAGTTAGTTGTAAACGGATTCTCAAAGCTACCATTTTAGCCAACTTCTTTGAGTTGTACCAAACCAATAAGAATGAACTATTGGATATCTGTCGGGAAGCCAAAAACCCTGTTTTAAAGGAAATTACCTGGTGGTTTAAGAAGAACTTTGCACTGTTATCAAAGCTATTCCCCGTCCACATTATGACGTTTGAATCAGCCGCCTTATTAACCCCGAACCAGCGCCGTTTGTATGACTATGTGGTGATTGATGAAGCTAGCCAGGTTTATCTAGAACGTGCCATTCCAATCTTATACCGTGGCGCTAAGTACATTATTGCTGGAGACACCAAACAATTAAAACCATCCAATTTCTTCCAAGCCCGGGCTGAGTATGATGTGGATGAAGAGTTTGAAGATGGTAACGTTGAAGCTGCGGTGCACTCGACTTCGTTACTACACTTTTTGAAGAACCGTTCGCGCATTTTAACGCTGCTCAAATTCCACTACCGCAGTGATTCCGCTAACCTAATTGCCTTTACTAATAACCGCATTTATAACAACGAGCTCATCTTTATGAATAAAGCAACCGCTGACAAGCAGGTCTTTATTGTCCATGATGTCATCGATGGTATTTGA", "aa": "MENLVQTQKQLVKAKQSYLSKIEQYVVSCKRILKATILANFFELYQTNKNELLDICREAKNPVLKEITWWFKKNFALLSKLFPVHIMTFESAALLTPNQRRLYDYVVIDEASQVYLERAIPILYRGAKYIIAGDTKQLKPSNFFQARAEYDVDEEFEDGNVEAAVHSTSLLHFLKNRSRILTLLKFHYRSDSANLIAFTNNRIYNNELIFMNKATADKQVFIVHDVIDGI", "aa_hexdigest": "d305f28155a62318e0b718863ee1659c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 42, "aa_cov": 0.1782608695652174, "hmm_cov": 1.0, "evalue": 1.2318559656922547e-09, "score": 33.72935485839844, "start": 100, "stop": 140 } ], "seq_stats": { "molecular_weight": 26809.62560000002, "isoelectric_point": 8.885886955261231 }, "id": "CNOCLNGIAL_395", "locus": "GCA000143945_00360" }, { "type": "cds", "sequence": "contig_1", "start": 203286, "stop": 203897, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "GTGAACGCTCAGGGCGAGTATGTCGGTTTGTTTGTCAAAAACATTGAAAACGTTCAGGGTGATGAACGGGACATTATTATCTTCTCGTTGGGCTATGACCGTTCTGTCAACAGCTATGGCCCGATTTCCAAGCAAGGTGGGGAGAACCGTTTGAATGTAGCGATTACCCGTGCTAAGCAGCGGATTGAACTGTTCAAGACTAACCGTGCTAGTGATTACAATGGTTTGAGCTCCAATTCCTTGGGTAGTAAGTTATTGGTGGAATACTTACTGTACTGTGAGGCAATGGCCAACAACCAGGGTGAGAGCTTAGACTTCCAAGCAACCCAAAAACAAGCACCCAAGGCCAAGTACGAATTGGAATTGGAGAACCAGTTCTTTAATGAGTTGGAACTTATTTTTGGTGAGCAGTTTACGATTAAACGCAATGTCAATGAGGGTGCATACAGCTTTAGCTTTGTCTTTTACTTTAACGAAAACCCTTACCTAGCGGTTGACTTTAACCCTGCTTTACCCCACTCCAGAAAGGAAGTGAGTGAAAACATTATTTACCGCGAACAATTTTTGAAAAAGCGGAAATGGAACCTCGTTAACATTTGGTTAGACGAGTGA", "aa": "MNAQGEYVGLFVKNIENVQGDERDIIIFSLGYDRSVNSYGPISKQGGENRLNVAITRAKQRIELFKTNRASDYNGLSSNSLGSKLLVEYLLYCEAMANNQGESLDFQATQKQAPKAKYELELENQFFNELELIFGEQFTIKRNVNEGAYSFSFVFYFNENPYLAVDFNPALPHSRKEVSENIIYREQFLKKRKWNLVNIWLDE", "aa_hexdigest": "542c68ba3f61a75ff8891bc9036adde9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 53, "aa_cov": 0.26108374384236455, "hmm_cov": 0.9433962264150944, "evalue": 1.0431654733387908e-09, "score": 33.67409133911133, "start": 10, "stop": 62 } ], "seq_stats": { "molecular_weight": 23528.12060000002, "isoelectric_point": 5.281274604797362 }, "id": "CNOCLNGIAL_396", "locus": "GCA000143945_00361" }, { "type": "cds", "sequence": "contig_1", "start": 204052, "stop": 204672, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "ATGAACAATCAATCAAATCACTTTACCAATCCTTTGTTACAACTGATTAAGAACGTTGCCGAGACCAAGAATTTAGCCATCGATGATGTTGTTTTGTGTCTTAAAACAGCATTAGAACAAACATACAAGAAGCATTTGAACTATGTCAATGTGGAAGTCAACATTGACTTTAACAAGGGCTTAATGCAAATTGAACAGCTCTTCGATGTTGTAGATGATAACAATGAGGATTATGATGACTTTTTGGAAATGCCTTTAAGTGAGGCTAAGAAGCTCAATCCGAACCTAGAAGTTGGCGGGGTGTTGCGCAAACCGGTTTCACTCAAAGACATTAAGGGCGATCTCATTAGCAAAATGGTATTACTCTTCAACCAAAAGATTAACGAAACAGCTTTTAAAACGGTGATGAGTGACTTCATTAATGAAGTCGGTCAGGTAATTGAAGCTAGAGTAGAGGACATTGATACGAACAAAGATGGTGGATTGAAGGGTTACATTGTTAACTTGGAAACCACCAAGGGTTACATGCCTAAACGTGAGTTGAGTAAGGGCGAAAAGCTAGATATTGGCAAGAAGTACCTCTTTGTGATTAAGGAAATCCAAAAGCAATCTTCGATGTGA", "aa": "MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALEQTYKKHLNYVNVEVNIDFNKGLMQIEQLFDVVDDNNEDYDDFLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMVLLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDGGLKGYIVNLETTKGYMPKRELSKGEKLDIGKKYLFVIKEIQKQSSM", "aa_hexdigest": "4064af306544f97da505e54264cad8e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 120, "aa_cov": 0.5776699029126213, "hmm_cov": 0.9583333333333334, "evalue": 7.000877445994065e-20, "score": 66.93895721435547, "start": 12, "stop": 130 } ], "seq_stats": { "molecular_weight": 23473.773300000008, "isoelectric_point": 5.225913429260252 }, "id": "CNOCLNGIAL_397", "locus": "GCA000143945_00362" }, { "type": "cds", "sequence": "contig_1", "start": 204715, "stop": 204921, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13184.12" ], "nt": "TTGCTAAATTCCAACACTCCCGAAATTGCTAACGGTACGATCGAAATTAAAAAGATGGAACGCTCTCCTGGTACCAAGTCTAAGGTGGCCGTGATTTCCAAAGACCCAGTAGTTGATCCGATTGCGGCCATTTTGGGTCCCAAGGGCGAGCGCATTCGCGGCATTAGTGAAGAGTTTAACGGTGAGATCATTGATATTGTCATCTGA", "aa": "MLNSNTPEIANGTIEIKKMERSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVI", "aa_hexdigest": "3cc5c7e3d8110d3a85095e519a519cb2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13184.12", "name": "KH_5", "length": 45, "aa_cov": 0.6617647058823529, "hmm_cov": 1.0, "evalue": 2.0861385334324725e-13, "score": 45.73484802246094, "start": 23, "stop": 67 } ], "seq_stats": { "molecular_weight": 7301.397599999999, "isoelectric_point": 5.311058235168456 }, "id": "CNOCLNGIAL_398", "locus": "GCA000143945_00363" }, { "type": "cds", "sequence": "contig_1", "start": 204943, "stop": 205674, "strand": "+", "frame": 1, "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "db_xrefs": [ "GO:0003700", "GO:0003723", "GO:0005829", "GO:0006353", "GO:0031564", "SO:0001217", "UniRef:UniRef50_P47387", "UniRef:UniRef90_P75591" ], "nt": "TTGGTCAACGCTGTTTTACCGGCTGAAGTGGTCGGTTACAACATCCTTCAAGACGATGAGCGCGATACCAGCATTGAAATCGTGGTACCCGCCAACCAGATTGCCAACGTCTTTGGTTTTAAAGGAATTAACATTCGCTTAATTAGTAACCTAACGGGTTGGAGTAGTGTGGACGTTTACACCGAAAAAGATGCCGCTGAACAAGGGATTGAATACACCCGGGTCAACTTTCAACCCCAAGGTATTTTTGGCATCAAAAAGCGCCGCGACAAGATCTCCAACAATCCACGCAACAACAACCAACAGCTCGCATCTGATAAGGTGTTTTACACTTCGAAGGCCAATGTGGTTGATGATGAGATCATAGTCGATCTAGCAAAGCAAGCGGAAGCCAAACGGGTCAAGCAAATCAAGCAGGAAGCAACTAAACCCGAGTTGCAATTACAACAAGAACTTAACCTCGAAGCAACCCCTAAGGTAGCTGCTCCAACCCCAACCCCTGCACCCCAACCAACTCCCGCACCAACTAAAGTTGAGCCAGTACCACCACCAGTAAGCGTTACACCTAAGCCAATCCCCAAGGTCAATAAGCCCAAGCCAGTGGTTAAACCAAAATCAGTCTTTTCGATTACGGTTGAAGCGGATGACAGTAAGACTAAACCAGAAAAGTCAAGCGCTAAAACTAACACCCCACAAACGAAACAAACCTTTGATAACTTTGACGATCTCTAG", "aa": "MVNAVLPAEVVGYNILQDDERDTSIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDAAEQGIEYTRVNFQPQGIFGIKKRRDKISNNPRNNNQQLASDKVFYTSKANVVDDEIIVDLAKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPPPVSVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL", "aa_hexdigest": "2160ce94dd7a92c2d39f420208ca3dfd", "start_type": "TTG", "rbs_motif": null, "genes": [ "nusA" ], "truncated": "5-prime", "pseudogene": { "start": 204643, "stop": 205674, "observations": { "mutation": [ 204670, 204919 ], "stop": [ 204670, 204919 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75591", "query_cov": 1.0, "subject_cov": 0.45, "identity": 0.992, "score": 374.0, "evalue": 5.66e-125, "gene_start": 1, "gene_end": 243, "reference_start": 298, "reference_end": 540, "reference_sequence": "MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALAQTYKKHLNYVNVEVNIDFNKGLMQIEQLFDVVDDNNEDYDDFLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMVLLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDGGLKGYIVNLETTKGYMPKRELSKGEKLDIGKKYLFVIKEIQKQSSMWPITLSRSDSRLLEFLLNSNTPEIANGTIEIKKMERSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVIWNEDKLKFLVNAVLPAEVVGYNILQDDERDTSIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDAAEQGIEFTRVNFQPQGIFGIKKRRDKISNNPRNNNQQLASDKVFYTSKANVVDDEIIVDLAKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPPPVSVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL" }, "paralog": false, "identity": 0.9912536443148688, "score": 576.0, "evalue": 9.37e-207, "description": "Internal stop codon at 204670, 204919. Nonsense mutation around 204670, 204919.", "psc": { "uniref90_id": "UniRef90_P75591", "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "uniref50_id": "UniRef50_P47387", "go_ids": [ "GO:0005829", "GO:0003700", "GO:0003723", "GO:0006353", "GO:0031564" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75591", "UniRef:UniRef50_P47387", "GO:0005829", "GO:0003700", "GO:0003723", "GO:0006353", "GO:0031564" ] }, "pscc": { "uniref50_id": "UniRef50_P47387", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47387" ], "product": "Transcription termination/antitermination protein NusA" } }, "id": "CNOCLNGIAL_399", "locus": "GCA000143945_00364" }, { "type": "cds", "sequence": "contig_1", "start": 205699, "stop": 205968, "strand": "+", "frame": 1, "gene": "ylxR", "product": "Transcription termination protein", "db_xrefs": [ "COG:AJ", "COG:COG2740", "KEGG:K07742", "RefSeq:WP_015344887.1", "SO:0001217", "UniParc:UPI0002B84478", "UniRef:UniRef100_A0AB38W8E2", "UniRef:UniRef50_A0AB38W8E2", "UniRef:UniRef90_A0AB38W8E2" ], "nt": "ATGCAAAGTTGGCGCCAATGTTTAGTTACCAGAAAGTCTTTTGCTAAGCCGCTACTTTTGCGTCTTGTAAAATTAGAGCATCAACTACGGGTGGATTTAGCGCAAACACTACCAGGTCGGGGGTATTATTTAAGTGTAGCTGCTTTAAAACTGGAGCGGAAAAACTTTAAAGCACTGTTACAAAAACGCTTAAAGGTAAGCTGTTCTGACGCAGAGCTAGATCACATTATTGCTTGTTTAAAGGAAAGGGAAAGCGATGTCAAAGCATAA", "aa": "MQSWRQCLVTRKSFAKPLLLRLVKLEHQLRVDLAQTLPGRGYYLSVAALKLERKNFKALLQKRLKVSCSDAELDHIIACLKERESDVKA", "aa_hexdigest": "4776d9d969246ce69f2cdc0f210ccdfa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B84478", "ncbi_nrp_id": "WP_015344887.1", "uniref100_id": "UniRef100_A0AB38W8E2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B84478", "RefSeq:WP_015344887.1", "UniRef:UniRef100_A0AB38W8E2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8E2", "uniref90_id": "UniRef90_A0AB38W8E2" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8E2", "gene": "YlxR", "product": "Transcription termination protein", "uniref50_id": "UniRef50_A0AB38W8E2", "cog_id": "COG2740", "cog_category": "AJ", "kegg_orthology_id": "K07742" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8E2" ], "product": "Transcription termination protein" }, "genes": [ "ylxR" ], "id": "CNOCLNGIAL_400", "locus": "GCA000143945_00365" }, { "type": "cds", "sequence": "contig_1", "start": 205955, "stop": 207808, "strand": "+", "frame": 2, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413", "KEGG:K02519", "RefSeq:WP_014325382.1", "SO:0001217", "UniParc:UPI0001DD64D7", "UniRef:UniRef100_A0AAX0SR00", "UniRef:UniRef50_P47388", "UniRef:UniRef90_P75590" ], "nt": "ATGTCAAAGCATAAACCACGTCATTTTCAAAAGAATAAGTTTGACAACCGGGCTAAAACGAGTGCCAAACAACAGTTTCGCCAGGTAAAAACCGGTGTTAAAGATGGTGTCTTTGTCTATAAGGGACCACTCACTGTTAGTGAGTTTTGTCTCAAAACCAATATTCCGACGGCCAACATCATCAAGCACTTCTTTTTAAATGGGGTTCCCTTAACCTTAAATTCGGTGTTATCGACCGAACAACTAGCTGACGCTTGTGTTAACTTTGGCTTTGACTTTAAGGTAGAAACCGAAATCACCCACGACAACATTATCTCCAACATTAAGTTTGACGATGATCCCACCCAGTTAAGTCCAAGACCCCCGATCGTTACCATTATGGGTCACGTGGATCACGGGAAAACATCCTTGTTAGACGCCATTCGCCAAACTAATACAGCAGCCAAAGAGTTTGGTGGCATTACCCAAAAGATTGGGGCGTACCAGGTCAAAAACCAGGAGGGCAAAACGATTACCTTCATTGATACCCCCGGTCATGAAGCCTTTACCGGCATGCGCGCCCGTGGTGCTCAAGTGACTGACATTGTTGTCTTAGTGGTAGCGGGTGATGATGGCTTAAAACAACAAACTGAGGAAGCGATTTCCCACGCCAAGAGTGCCAAAACCCCGATCATTGTCTTTATTAACAAGATGGATAAACCAACCGCTAACCCCGACATGGTGATCCAACAGCTCAACAAGTTTGATTTAGTCCCTGAAGAATGGGGTGGTGATACCATCTTTGTCAAGGGCAGTGCCTTAACCAAAGAGGGGATCCAGGAGCTCTTGGACAGTATTCTGTTAGTAGCGGAAGTGGAAGATTACAAGGCCAACTTTAACGCCCACTCATCGGGTTATGCGATTGAGGTGCAAACCACCAAGGGGTTGGGTCCCACCGCTACCATTATTGTTAAACGTGGTACCCTAAAGATTGGTGACATCGTTGTCTTGGGTCCCGCTTGGGGTAAGGTTCGTACCATGCAAGACGAAAACGGAGTCCATCTCCAAGAAGCTATGCCCTCTAAACCAGTACAAATTTCGGGGTTTGACATTGTCCCAGTTGCTGGGGAAAAATTCATTGTCTTTGACGATGAAAAGGATGCGAAGTTAATTGCCAATAAGTTCCGCGAACAGCAAAAACAAAAGCTCAATACCACCCAAATTAACGAGGTGCTCAAACAAAAGATTAAGAGCAAGGAAATTAAGGTTTTAAATCTGATTTTTAAGGTTGACAGTGATGGTAGTTTAGCGGCCATTAAACAAGCTATGCAGAGCATTGATGTGCCGGGGATGAGCGTCAACATTATCCACTCAGGTGTTGGTTTAATCTCGGAAAACGACATTATGTTAGCCAAGGCGTCCGGGGCATTACTGTTTAGCTTGAACCTCGGTTTAAGTCAAGTTGTTAAGAACATTGCTAGTCTTCAAGGAGTGAAGGTCGATGTCCACTACCACATCCCTAAGTTAGCGGAAGAGATCGAAAACATCTTAAAGGGCCAGTTAGAACCAGTGTACGAAGACGTGGAACTGGGGCGTGCTGAAGTGTTACAACTCTGGTACCACTCCAAGGTGGGGCACATTGCTGGTACCTTAGTTAAAACAGGTAAGGTTAAACGGGGCGCCTTGTGTAAGCTGTTACGCCGTAACGAAACCATCTATGAAGGCCGGGTGGACTCATTGAAGAGTGAAAAGAACCCGGTCAACCAGATGGAAGCGGGGAAGAACTGCGGTATTGTTATTAATGGGTGTGAGGACATTCAGGTGTGCGACATTATCTTGGTGTACGAAAAGCAGGAGGTCAAGTCCAAAAGTTAA", "aa": "MSKHKPRHFQKNKFDNRAKTSAKQQFRQVKTGVKDGVFVYKGPLTVSEFCLKTNIPTANIIKHFFLNGVPLTLNSVLSTEQLADACVNFGFDFKVETEITHDNIISNIKFDDDPTQLSPRPPIVTIMGHVDHGKTSLLDAIRQTNTAAKEFGGITQKIGAYQVKNQEGKTITFIDTPGHEAFTGMRARGAQVTDIVVLVVAGDDGLKQQTEEAISHAKSAKTPIIVFINKMDKPTANPDMVIQQLNKFDLVPEEWGGDTIFVKGSALTKEGIQELLDSILLVAEVEDYKANFNAHSSGYAIEVQTTKGLGPTATIIVKRGTLKIGDIVVLGPAWGKVRTMQDENGVHLQEAMPSKPVQISGFDIVPVAGEKFIVFDDEKDAKLIANKFREQQKQKLNTTQINEVLKQKIKSKEIKVLNLIFKVDSDGSLAAIKQAMQSIDVPGMSVNIIHSGVGLISENDIMLAKASGALLFSLNLGLSQVVKNIASLQGVKVDVHYHIPKLAEEIENILKGQLEPVYEDVELGRAEVLQLWYHSKVGHIAGTLVKTGKVKRGALCKLLRRNETIYEGRVDSLKSEKNPVNQMEAGKNCGIVINGCEDIQVCDIILVYEKQEVKSKS", "aa_hexdigest": "91d8a369556c2040bd580e7b0a30a727", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64D7", "ncbi_nrp_id": "WP_014325382.1", "uniref100_id": "UniRef100_A0AAX0SR00", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64D7", "RefSeq:WP_014325382.1", "UniRef:UniRef100_A0AAX0SR00" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SR00", "uniref90_id": "UniRef90_P75590" }, "psc": { "uniref90_id": "UniRef90_P75590", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_P47388", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_P47388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47388" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "CNOCLNGIAL_401", "locus": "GCA000143945_00366" }, { "type": "cds", "sequence": "contig_1", "start": 207811, "stop": 208161, "strand": "+", "frame": 1, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024", "KEGG:K02834", "RefSeq:WP_010874513.1", "SO:0001217", "UniParc:UPI00001331B1", "UniRef:UniRef100_P75589", "UniRef:UniRef50_P47389", "UniRef:UniRef90_P75589" ], "nt": "ATGGCTAGTTACAAAAAGGAACGTCTGGAAAACGACATTATTCGTTTAATTAACCGCACTGTTATTCACGAAATCTACAACGAAACGGTCAAAACCGGTCATGTAACCCACGTGAAACTATCGGATGATTTACTCCATGTAACAGTTTACCTAGACTGTTATAACCGTGAGCAAATTGACCGCGTGGTGGGTGCCTTTAACCAGGCCAAAGGGGTGTTTAGCCGCGTGTTAGCACACAATTTGTACTTAGCCAAAGCCGTGCAAATCCATTTTGTCAAGGACAAAGCAATTGATAACGCAATGCGCATTGAGTCTATCATCAACTCCCTTAAAAAATCAAAGCCTAATTAA", "aa": "MASYKKERLENDIIRLINRTVIHEIYNETVKTGHVTHVKLSDDLLHVTVYLDCYNREQIDRVVGAFNQAKGVFSRVLAHNLYLAKAVQIHFVKDKAIDNAMRIESIINSLKKSKPN", "aa_hexdigest": "ea805a36f7a0eafb24a3f0e2c3411375", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001331B1", "ncbi_nrp_id": "WP_010874513.1", "uniref100_id": "UniRef100_P75589", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001331B1", "RefSeq:WP_010874513.1", "UniRef:UniRef100_P75589" ] }, "ips": { "uniref100_id": "UniRef100_P75589", "uniref90_id": "UniRef90_P75589" }, "psc": { "uniref90_id": "UniRef90_P75589", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_P47389", "cog_id": "COG0858", "cog_category": "J", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024" ] }, "pscc": { "uniref50_id": "UniRef50_P47389", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47389" ], "product": "Ribosome-binding factor A" }, "genes": [ "rbfA" ], "id": "CNOCLNGIAL_402", "locus": "GCA000143945_00367" }, { "type": "cds", "sequence": "contig_1", "start": 208177, "stop": 208998, "strand": "+", "frame": 1, "gene": null, "product": "putative MG144-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75588", "UniRef:UniRef90_P75588" ], "nt": "ATGAAACCACCTAAAAAGCCAGTCAACCCACCGTTTGGTAAGCTAACTGATTCTAAAGCTGGAGTAGTCAAAGCTACCTCTAGTCAAGAGACCAAAAAAGTAGCTGACACCAAACCCAAAAATAAGGGTGGTTTGTTTAGCTTCTTTAAAAAAGACAAAACGGAAAAACCAGCTAAAGCAGCCAAACCCAAAGATGCCTTTAAGAGTGCGATAGCTGAATTGAATCCGAAAGCCAATCCTAAGACAGTCAAAGCGGATGTTGCACCGGCTAAAATTCCCCACAGCGATAAGGGTACGGTTACCCCTGTAGAGTTAAAACCCCAAACGGAAGCACCGTTACCACCAGGAGTTAAACAACCTGATCCTAAAAAAGATAAACCTAAGGGCGGTTTATTTGGCTTCTTCAAAAAAGATAAGAACAAAGATGTCAAAAAGGAGCCTGCTAAGCCAGCTACTCCAGTAAAAACTGAACCAACCTCACCTAAGGTAGAACCAACTAAGGTCAAACCACCGGGCGGTGTACCGACTAAGCCGGTAGTGGAAAAACCAGTGTCAGCTCAACCAACGGTACCATTACAACCAGAACCTACTTTCCCAGTCAAAGCAGCACCACTACCACCAGGTGTAAAAGCAGAACCGGAAAGCAAAAAACGCTTTGGTTTGTTTAAGGCATTCCAGAAAGATGACGCTAAACAACCCAAGCAAAAGCTCAATCTCCAAGACCAACAGGATCGCTTTATTGATGACCCCACCGCCAAAAAGCACTTTAGCGCCTTTAACCAAAAAGTTGGTAACTTACTGAAGGACAAAAAAGACCCGTAA", "aa": "MKPPKKPVNPPFGKLTDSKAGVVKATSSQETKKVADTKPKNKGGLFSFFKKDKTEKPAKAAKPKDAFKSAIAELNPKANPKTVKADVAPAKIPHSDKGTVTPVELKPQTEAPLPPGVKQPDPKKDKPKGGLFGFFKKDKNKDVKKEPAKPATPVKTEPTSPKVEPTKVKPPGGVPTKPVVEKPVSAQPTVPLQPEPTFPVKAAPLPPGVKAEPESKKRFGLFKAFQKDDAKQPKQKLNLQDQQDRFIDDPTAKKHFSAFNQKVGNLLKDKKDP", "aa_hexdigest": "25b777252a512497ed1257c57cb16c31", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 208177, "stop": 209299, "observations": { "insertion": [ 208984 ], "stop": [ 209021 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75588", "query_cov": 0.9926739926739927, "subject_cov": 0.6741293532338308, "identity": 1.0, "score": 385.0, "evalue": 9.12e-131, "gene_start": 1, "gene_end": 271, "reference_start": 1, "reference_end": 271, "reference_sequence": "MKPPKKPVNPPFGKLTDSKAGVVKATSSQETKKVADTKPKNKGGLFSFFKKDKTEKPAKAAKPKDAFKSAIAELNPKANPKTVKADVAPAKIPHSDKGTVTPVELKPQTEAPLPPGVKQPDPKKDKPKGGLFGFFKKDKNKDVKKEPAKPATPVKTEPTSPKVEPTKVKPPGGVPTKPVVEKPVSAQPTVPLQPEPTFPVKAAPLPPGVKAEPESKKRFGLFKAFQKDDAKQPKQKLNLQDQQDRFIDDPTAKKHFSAFNQKVGNLLKDKKTRNRDWKIVGWIHGLILLFFIPLLAIMNKFVTLPAQSYPAVSLQVSINNALWGIAIFVIANIALPFITMFVLFLTGVRDVHASRPVHYFIWVLMLLNLTFLVISCCLLAAAYANLDLYNIWRNLQALDPNN" }, "paralog": false, "identity": 0.9973190348525469, "score": 602.0, "evalue": 1.41e-218, "description": "Internal stop codon at 209021. Frameshift due to insertion around 208984.", "psc": { "uniref90_id": "UniRef90_P75588", "product": "Uncharacterized protein MG144 homolog", "uniref50_id": "UniRef50_P75588", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75588", "UniRef:UniRef50_P75588", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75588", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75588" ], "product": "Uncharacterized protein MG144 homolog" } }, "id": "CNOCLNGIAL_403", "locus": "GCA000143945_00368" }, { "type": "cds", "sequence": "contig_1", "start": 209069, "stop": 209353, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTGTGACCCTCCCAGCACAAAGCTATCCTGCTGTATCACTACAAGTATCGATTAACAATGCGTTGTGGGGGATTGCGATCTTTGTGATAGCCAACATTGCTCTACCCTTCATTACGATGTTCGTGCTCTTCTTGACTGGGGTCAGAGATGTGCATGCTAGTCGGCCAGTGCACTATTTCATCTGGGTGTTAATGTTGTTAAACCTTACCTTCTTAGTAATTAGTTGTTGTTTACTGGCTGCTGCTTATGCTAACTTAGACATTTACAACATCTGA", "aa": "MNKFVTLPAQSYPAVSLQVSINNALWGIAIFVIANIALPFITMFVLFLTGVRDVHASRPVHYFIWVLMLLNLTFLVISCCLLAAAYANLDIYNI", "aa_hexdigest": "78a3b6bbfaab2e4ae3a565bb6d992f8c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10504.50679999999, "isoelectric_point": 7.825124168395997 }, "id": "CNOCLNGIAL_404", "locus": "GCA000143945_00369" }, { "type": "cds", "sequence": "contig_1", "start": 209399, "stop": 209902, "strand": "+", "frame": 2, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0008531", "GO:0009231", "GO:0009398", "KEGG:K11753", "SO:0001217", "UniRef:UniRef50_P75587", "UniRef:UniRef90_P75587" ], "nt": "ATGCAGCAAACCTTGATCATCGGTGCTTTTGATGGTTTACACAAGGGTCATCAATTGTTAGCACAAGCGGCTAGTGGTCCAGTGGTGGCCTTGTTAATTGCTAACATTCCCAGTCTACAAAGTGACTGGCTATACGAACCGAAGCAACGGCAAGTGCAGTTACAACAACACTTTCAGTCCGTGGTGCACAGCTATGATGTGATTGAACACAACATTAGTGCCCAAGCGTTTTTCGATCAAATCATTAGCCCACTACGGTGTCAGCAATTAGTCGTGGGTGCGGACTTTTGCTTTGGTAAAGACAACCAGAATGCTGATTTTTTAAGACGTTTATTTCCCAATACCACGATTATTCCCAAAGACAGTCAAACGCTGTCCAGTTCTACCATTCGCCAATGGTTAAAGCAGGGCCAGATAGAACAAGCCAACGCCGTGTTATTGGAACCCTACTTTAGGGAAGGGGTGGTTATTCGTGGTAACCAGCAAGCCCGGTTTTTGGGCTGA", "aa": "MQQTLIIGAFDGLHKGHQLLAQAASGPVVALLIANIPSLQSDWLYEPKQRQVQLQQHFQSVVHSYDVIEHNISAQAFFDQIISPLRCQQLVVGADFCFGKDNQNADFLRRLFPNTTIIPKDSQTLSSSTIRQWLKQGQIEQANAVLLEPYFREGVVIRGNQQARFLG", "aa_hexdigest": "3f984b340a4f108a656631047a50588b", "start_type": "ATG", "rbs_motif": null, "genes": [ "ribF" ], "truncated": "3-prime", "pseudogene": { "start": 209399, "stop": 210205, "observations": { "mutation": [ 209900 ], "stop": [ 209900 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75587", "query_cov": 1.0, "subject_cov": 0.620817843866171, "identity": 1.0, "score": 332.0, "evalue": 6.87e-114, "gene_start": 1, "gene_end": 167, "reference_start": 1, "reference_end": 167, "reference_sequence": "MQQTLIIGAFDGLHKGHQLLAQAASGPVVALLIANIPSLQSDWLYEPKQRQVQLQQHFQSVVHSYDVIEHNISAQAFFDQIISPLRCQQLVVGADFCFGKDNQNADFLRRLFPNTTIIPKDSQTLSSSTIRQWLKQGQIEQANAVLLEPYFREGVVIRGNQQARFLGWPTANITLKPYMVPLRCGSYVITVTYHQTNYPGVGFISYKNDQLVCETHLIGFSGDLYGKQLRFTFNQFIRPQQKFSGVQALQKAISGDLKQAQKWFAQSTN" }, "paralog": false, "identity": 0.996268656716418, "score": 539.0, "evalue": 1.15e-197, "description": "Internal stop codon at 209900. Nonsense mutation around 209900.", "psc": { "uniref90_id": "UniRef90_P75587", "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_P75587", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "go_ids": [ "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0008531", "GO:0009231", "GO:0009398" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75587", "EC:2.7.1.26", "EC:2.7.7.2", "UniRef:UniRef50_P75587", "COG:COG0196", "COG:H", "KEGG:K11753", "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0008531", "GO:0009231", "GO:0009398" ] }, "pscc": { "uniref50_id": "UniRef50_P75587", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75587" ], "product": "Bifunctional riboflavin kinase/FMN adenylyltransferase" } }, "id": "CNOCLNGIAL_405", "locus": "GCA000143945_00370" }, { "type": "cds", "sequence": "contig_1", "start": 209933, "stop": 210208, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01687.23" ], "nt": "ATGGTGCCGTTGCGCTGTGGGAGCTATGTGATTACGGTGACTTACCACCAAACCAATTACCCGGGTGTAGGGTTTATCAGTTACAAAAACGACCAGTTAGTCTGTGAAACCCACTTAATTGGTTTTAGTGGTGATCTTTACGGTAAACAACTACGCTTTACCTTCAACCAGTTTATTCGCCCCCAGCAAAAGTTTAGTGGGGTGCAAGCGTTGCAAAAAGCGATTAGTGGTGATTTAAAGCAAGCGCAGAAGTGGTTTGCTCAATCAACAAATTAA", "aa": "MVPLRCGSYVITVTYHQTNYPGVGFISYKNDQLVCETHLIGFSGDLYGKQLRFTFNQFIRPQQKFSGVQALQKAISGDLKQAQKWFAQSTN", "aa_hexdigest": "39d300f6a3c9eeaae46e8384c823eb85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01687.23", "name": "Flavokinase", "length": 87, "aa_cov": 0.9010989010989011, "hmm_cov": 1.0, "evalue": 1.1241331751934254e-19, "score": 66.33644104003906, "start": 5, "stop": 86 } ], "seq_stats": { "molecular_weight": 10353.698899999992, "isoelectric_point": 9.469003486633301 }, "id": "CNOCLNGIAL_406", "locus": "GCA000143945_00371" }, { "type": "cds", "sequence": "contig_1", "start": 210234, "stop": 210683, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGGAAAGTGCCCCCAGTGGTGGCCTGTTGGCCTTAATTATTATCAGTATTATCCTCCTGGCTTGTATTAGTGCCGTGGTTTCGGCTTACGAAACCGCCATTACCTCAATTACATCATACAAGTGGAGCAACTATGTCAAAACGCATAACAAGCAAAAGAAGTTAACGACCAAGATTGTTAACCACTTCCAAAAGAACTATTCGGCTTGTTTAATTACCATCTTAGTGGCCAATAACATTGTCGCCATTTTGGTTTCCAACATCCTCTTTTTGGCTTTAGATCAATCGATTAAGAATCCGGCCATTTCCAGTGCCTTGAACCTCTTAATTAGTGGGGTGTTATTGTTAATGCTGTGTGAAATTACTCCCAAAACCTTAGCACGGATTAACATCATCCGCGTCTTGGTCTACTTTGCGGTGGTAGTGTATTTTTTCTACATCCTTTTTTGA", "aa": "MESAPSGGLLALIIISIILLACISAVVSAYETAITSITSYKWSNYVKTHNKQKKLTTKIVNHFQKNYSACLITILVANNIVAILVSNILFLALDQSIKNPAISSALNLLISGVLLLMLCEITPKTLARINIIRVLVYFAVVVYFFYILF", "aa_hexdigest": "ad7724b1de6b65499b3fe5f323baff98", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 135, "aa_cov": 0.9060402684563759, "hmm_cov": 0.9629629629629629, "evalue": 1.514559925900051e-20, "score": 69.0667495727539, "start": 15, "stop": 149 } ], "seq_stats": { "molecular_weight": 16423.589599999985, "isoelectric_point": 9.484218025207518 }, "id": "CNOCLNGIAL_407", "locus": "GCA000143945_00372" }, { "type": "cds", "sequence": "contig_1", "start": 210879, "stop": 211508, "strand": "+", "frame": 3, "gene": "mpfA", "product": "UPF0053 MG146-like protein", "db_xrefs": [ "COG:COG1253", "COG:PT", "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q49399", "UniRef:UniRef90_P75586" ], "nt": "GTGGTGTACTGTTATGAAGGTGATCCAGTCAAGACCATTAAGGAAAAGTTCTTGCACGGCCAGTTTTCCAGAATGCCCGTACTCGACCAGACAAGCAATGAAGTGGTTGGTTTTATCCACCTCAAGGACTTGTTTAGTTCACTCGAAAAGAGCAACGAGCCGTTTGTTTTACAGGAGTTATTGTATCCAGCTGTTTTAGTAAGTAACACCACACCCATCAAGCAAGCTTTGCGCCAAATGCGGTTACACCGGGCCCACTTAGCGGTCGTGCAGGACAAACACCACCACACGATCGGGATTGTTTCTATGGAAGACATCATTGAGGAATTAGTCGGGGAAATTTACGATGAACATGATGAGGTGGAGGCAGTGCAAACGTTGGACAACAACACCTGGTTAGTGTTACCGAACGTAAAAGCCGCCTTTTTCTTTAACAAGTGGATTAAACGGGATTTAGTAAAATCAAAAAACATGACGATCCAACGTTACTTGGCATCGTTTGAAAATGATGGGTTAAACACCCAGAATAAACTAGAAACGCCGTGGTTTATTGCTGAAGCAATTGTCGACAGCGAGAACCCCGAGCAAATTCGGTACGAAATTCGCAAAAAGAGTGATGTTGTGGACTAG", "aa": "MVYCYEGDPVKTIKEKFLHGQFSRMPVLDQTSNEVVGFIHLKDLFSSLEKSNEPFVLQELLYPAVLVSNTTPIKQALRQMRLHRAHLAVVQDKHHHTIGIVSMEDIIEELVGEIYDEHDEVEAVQTLDNNTWLVLPNVKAAFFFNKWIKRDLVKSKNMTIQRYLASFENDGLNTQNKLETPWFIAEAIVDSENPEQIRYEIRKKSDVVD", "aa_hexdigest": "1382fc4d7ae0a4de2be93c9c47d66841", "start_type": "GTG", "rbs_motif": null, "genes": [ "mpfA" ], "truncated": "5-prime", "pseudogene": { "start": 210579, "stop": 211508, "observations": { "mutation": [ 210681, 210870 ], "stop": [ 210681, 210870 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75586", "query_cov": 1.0, "subject_cov": 0.49061032863849763, "identity": 0.995, "score": 414.0, "evalue": 6.5e-143, "gene_start": 1, "gene_end": 209, "reference_start": 218, "reference_end": 426, "reference_sequence": "MTYGKCPSGGLLALIIISIILLACISAVVSAYETAITSITSYKWSNYVKTHNKQKKLTTKIVNHFQKNYSACLITILVANNIVAILVSNILFLALDQSIKNPAISSALNLLISGVLLLMLCEITPKTLARINIIRVLVYFAVVVYFFYILFWPITKLASIIFAKYEKAPPVSRRDVYFFIDEIEQNGLFTKEDGQLIKRTLIFDQVLVDQIMIKWNRVVYCYEGDPVKTIKEKFLHGQFSRMPVLDQTSNEVVGFIHLKDLFSSLEKSNEPFVLQELLYPAVLVSNTTPIKQALRQMRLHRAHLAVVQDKHHHTIGIVSMEDIIEELVGEIYDEHDEVEAVQTLDNNTWLVLPNVKAAFFFNKWIKRDLVKSKNMTIQRYLASFENDGLNTQNKLETPWFIAEAIVDSENPEQIRYEIRKKSDVVD" }, "paralog": false, "identity": 0.9935275080906149, "score": 608.0, "evalue": 1.06e-221, "description": "Internal stop codon at 210681, 210870. Nonsense mutation around 210681, 210870.", "psc": { "uniref90_id": "UniRef90_P75586", "gene": "MpfA", "product": "UPF0053 protein MG146 homolog", "uniref50_id": "UniRef50_Q49399", "cog_id": "COG1253", "cog_category": "PT", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75586", "UniRef:UniRef50_Q49399", "COG:COG1253", "COG:PT", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q49399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49399" ], "product": "UPF0053 protein MG146" } }, "id": "CNOCLNGIAL_408", "locus": "GCA000143945_00373" }, { "type": "cds", "sequence": "contig_1", "start": 211495, "stop": 212232, "strand": "+", "frame": 1, "gene": null, "product": "MPN160 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47393", "UniRef:UniRef90_P75585" ], "nt": "ATGTTGTGGACTAGAGCCTTAATCTTAGAGTTAAAAACCAATAAGCAAAGCCGCTTGTTGTGGTTACTTGCAATTCCCTTACTAATATCGTTAACCTTACTAACGTACGGTTTAGTGTTGTTTAGTTCCAGTGGTCGAATTGATCATGGTGATCACTTCCACTTACGTGAGCGCTTTGTGTTAACGACCGAAGAGTTAGTTACCTTTGTGGTAGCGAGTGTCGTGTTTGCGCTAACTGTTGCCTTGTTTGGGCTGGGTTGCTGGAAGTTGCTCCAAGGTCCAAAAGTGGATAGAACAAACATAAAGCTAGCAAACAGCAATCCTGCTCCCCAAGCCGTGGTATTGCAAGCTGATTGTGATCACTTTCAGGTAGGGGATCACTGTGTGTTTAGCGCTGAAAAGCAACACTTTAAACAACAGTTCAAACAGGACTTTTTGGGTAAAAGTAAGTTTTCCTTTCGCAATGAACTGTACCGTTTTTGTCTCATTGGTGTGTTAATTTCGCTGAATTTAGCGCTCAGTATGGTGGAAATCCCCGGCATTGTTCTACCGTGGGGCAGTTCAATCCAGTTCCGCTTTTTTAACACCGCCATCTTGTTTATTGCGATTCGCTTTGTTGGGTTACTCTCCACATCGTTAATTGCGATTATTACTCCATGGATTCACCTCCTCCTTCACCCCGTTCATACCCCGATTAGTACCGTTTTTTACATGGGCAATGACTTGGTAGTTCTGTGA", "aa": "MLWTRALILELKTNKQSRLLWLLAIPLLISLTLLTYGLVLFSSSGRIDHGDHFHLRERFVLTTEELVTFVVASVVFALTVALFGLGCWKLLQGPKVDRTNIKLANSNPAPQAVVLQADCDHFQVGDHCVFSAEKQHFKQQFKQDFLGKSKFSFRNELYRFCLIGVLISLNLALSMVEIPGIVLPWGSSIQFRFFNTAILFIAIRFVGLLSTSLIAIITPWIHLLLHPVHTPISTVFYMGNDLVVL", "aa_hexdigest": "51ce5f08758fd1099edbd881ce19b60a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 211495, "stop": 212535, "observations": { "mutation": [ 212230 ], "stop": [ 212230 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75585", "query_cov": 1.0, "subject_cov": 0.649867374005305, "identity": 1.0, "score": 471.0, "evalue": 1.61e-165, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 245, "reference_sequence": "MLWTRALILELKTNKQSRLLWLLAIPLLISLTLLTYGLVLFSSSGRIDHGDHFHLRERFVLTTEELVTFVVASVVFALTVALFGLGCWKLLQGPKVDRTNIKLANSNPAPQAVVLQADCDHFQVGDHCVFSAEKQHFKQQFKQDFLGKSKFSFRNELYRFCLIGVLISLNLALSMVEIPGIVLPWGSSIQFRFFNTAILFIAIRFVGLLSTSLIAIITPWIHLLLHPVHTPISTVFYMGNDLVVLWIFYFFYYHIFKAEVKQTTTVVNNKEFSQLVNTHKTKVAKALALIPVNLICGFIEGLGFYVGYFLILGKFGSVGHKIFYDSQANRDLINSANVIYFLLTTTTIFSLKYLFELLFFYSVEKGILNISRHFGLY" }, "paralog": false, "identity": 0.9971098265895953, "score": 687.0, "evalue": 3.11e-253, "description": "Internal stop codon at 212230. Nonsense mutation around 212230.", "psc": { "uniref90_id": "UniRef90_P75585", "product": "MPN160 family protein", "uniref50_id": "UniRef50_P47393", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75585", "UniRef:UniRef50_P47393", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47393" ], "product": "Uncharacterized protein MG147" } }, "id": "CNOCLNGIAL_409", "locus": "GCA000143945_00374" }, { "type": "cds", "sequence": "contig_1", "start": 212353, "stop": 212628, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCGTTAATTCCAGTTAATTTAATCTGTGGTTTTATTGAGGGTTTGGGTTTCTATGTCGGTTACTTCTTGATTTTGGGTAAATTTGGTAGTGTAGGGCATAAGATTTTCTATGACAGTCAAGCTAACCGTGACCTAATTAACAGTGCTAACGTGATCTATTTCTTACTCACTACTACCACCATTTTTAGCTTAAAGTACCTGTTTGAACTACTGTTCTTTTACAGTGTCGAGAAGGGTATACTTAACATTTCGCGCCACTTTGGTTTGTATTAA", "aa": "MALIPVNLICGFIEGLGFYVGYFLILGKFGSVGHKIFYDSQANRDLINSANVIYFLLTTTTIFSLKYLFELLFFYSVEKGILNISRHFGLY", "aa_hexdigest": "11c90ec030d761a2f8b55e1f1a9cbb06", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10391.103999999994, "isoelectric_point": 7.863224983215332 }, "id": "CNOCLNGIAL_410", "locus": "GCA000143945_00375" }, { "type": "cds", "sequence": "contig_1", "start": 212782, "stop": 213033, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGACGTCAAATACTTACAGGTCGAAATTGTCGATAAAAACACCATTCGCCTCCAGGAAGATGGCAAAAAGGGTCAACAAATTAGGTTAGATCAAATTATTAAGGTTGATCAAAGCAACATCTTAAACACTTTGGAGCAGGCACAACGCGAGGCATACGAAAGGGAGGCGCAAAGCCGTTACCAAACTAAGTTGGCCAAAGAACTGAGTGAAAAAGACAACACTTTTTTAAAGCAAGAAGCAGCTTGA", "aa": "MSDVKYLQVEIVDKNTIRLQEDGKKGQQIRLDQIIKVDQSNILNTLEQAQREAYEREAQSRYQTKLAKELSEKDNTFLKQEAA", "aa_hexdigest": "6b6a89f0b9f9f73823c7475d6f530285", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9712.791099999993, "isoelectric_point": 5.46429615020752 }, "id": "CNOCLNGIAL_411", "locus": "GCA000143945_00376" }, { "type": "cds", "sequence": "contig_1", "start": 213214, "stop": 213360, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATACGAACGTAAAGCAAATAAAACTAAGGAAGAAAATCAACAAGAATTAGAAAGACAACGCCAGTACTTTTTAGAACAGTTAGAACAAGCCCAAAAGGACATTGAAGAGCTCAAAACGCGTGAAGAGCGTTTTAGTAAGTGA", "aa": "MEYERKANKTKEENQQELERQRQYFLEQLEQAQKDIEELKTREERFSK", "aa_hexdigest": "d147e97b9e9c59a795c2412b96d1a0c6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6106.661499999998, "isoelectric_point": 5.169983863830565 }, "id": "CNOCLNGIAL_412", "locus": "GCA000143945_00377" }, { "type": "cds", "sequence": "contig_1", "start": 213385, "stop": 214119, "strand": "+", "frame": 1, "gene": null, "product": "putative MG148-like protein", "db_xrefs": [ "COG:COG4487", "COG:S", "SO:0001217", "UniRef:UniRef50_P75584", "UniRef:UniRef90_P75584" ], "nt": "TTGGAAAAGTGGTGTTGGGAAGCTTACAAAAATTACGAAGAGTTGTTCCAAAACTGTGTGTTTGCCCCTTACAGTGAGCTAGTTAAGGGTGCCAAAAAGAGCATAGGTGTAGAAGACGATGAGAGCAATATTAACGAAAAGGCTGACTTTATCTTTCAGGTATTTAATCCCAATAATGACAAAGAACCCTTCTTTTCAATTTGCTGTGAAATGAAAACAGAGTTTACTGAAAGCAAGAGTCGCACTAAAAACGAAGATCATGTCAAAAAACTGATTGCCGACGCTAAGCGCGCTAAGTGCCAATACGGTTTTTTAGTAAGTGAATTGGAACTCAACACTGAAAACGATGTCCAAGTGCAACGGATGCATACCTCGAACAGTGAAGTGGAGGTTTACCTAGTGCGCCCAATGTTCTTCATTGTGATGTTGCGGTTGTTTTACTTTTTGGCCAAAAAGATGTTTGCCCAAGTTGATGTAAACAGCGAGTACTTAGATAAAGAAGCTTTAAATGCTAGCTTTAGCGATTTAAAGAAAAGCTTACTTGAAAAAACCTTTACTGATCTCAACAAGGTCTTTCAAAACAACATTGATGAACTAGAAAAGATTGAAGGATTAGTAACAAAGTTGAAAGCCGCTAATGAAAAGGCCTTAAATAGTCGTTTAAATAACTGGGAAGAAAAGATCCGTAAGTTTGAATTTAAGCTCAATAAAGACATTGTTAAAAAGCTTGAATAA", "aa": "MEKWCWEAYKNYEELFQNCVFAPYSELVKGAKKSIGVEDDESNINEKADFIFQVFNPNNDKEPFFSICCEMKTEFTESKSRTKNEDHVKKLIADAKRAKCQYGFLVSELELNTENDVQVQRMHTSNSEVEVYLVRPMFFIVMLRLFYFLAKKMFAQVDVNSEYLDKEALNASFSDLKKSLLEKTFTDLNKVFQNNIDELEKIEGLVTKLKAANEKALNSRLNNWEEKIRKFEFKLNKDIVKKLE", "aa_hexdigest": "0d53eec1d7093812876b189bce4ba508", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 213085, "stop": 214119, "observations": { "mutation": [ 213358 ], "stop": [ 213358 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75584", "query_cov": 1.0, "subject_cov": 0.5483146067415731, "identity": 0.996, "score": 470.0, "evalue": 3.33e-164, "gene_start": 1, "gene_end": 244, "reference_start": 202, "reference_end": 445, "reference_sequence": "MSDVKYLQVEIVDKNTIRLQEDGKKGQQIRLDQIIKVDQSNILNTLEQAQREAYEREAQSRYQTKLAKELSEKDNTFLKQKAAWNQAHNTQIQQLYQQITNLENQVNNIKRETESKKDNEYQQQIVKLETQLQSIKKETESQKDLEYERKANKTKEENQQELERQRQYFLEQLEQAQKDIEELKTREERFSKWAIAKKGKELEKWCWEAYKNYEELFQNCVFAPYSELVKGAKKSIGVEDDESNINEKADFIFQVFNPNNDKEPFFSICCEMKTEFTESKSRTKNEDHVKKLIADAKRAKCQYGFLVSELELNTENDVQVQRMHTSNSEVEVYLVRPMFFIVMLRLFYFLAKKMFAQVDVNSEYLDKEALNASFSDLKKSLLEKTFTDLNKVFQNNIDELEKIEGLVTKLKAANEKALNSRLNNWEEKIRKFEFKLNKDIVKKLE" }, "paralog": false, "identity": 0.9767441860465116, "score": 620.0, "evalue": 1.12e-225, "description": "Internal stop codon at 213358. Nonsense mutation around 213358.", "psc": { "uniref90_id": "UniRef90_P75584", "product": "Uncharacterized protein MG148 homolog", "uniref50_id": "UniRef50_P75584", "cog_id": "COG4487", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75584", "UniRef:UniRef50_P75584", "COG:COG4487", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_P75584", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75584" ], "product": "Uncharacterized protein MG148 homolog" } }, "id": "CNOCLNGIAL_413", "locus": "GCA000143945_00378" }, { "type": "cds", "sequence": "contig_1", "start": 214241, "stop": 214594, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTAAATCTGCGATTTCCTAGCTATTTTTTACCCGTCGTTGCCGCTAGTGCTTTCCTTGTTAGTTGCGCCACACCTAACACTTACGAAGTACAACGCGCTGCCTTGATTCAACTAGTTGAAGAAGACGAACGACAAAACTACATTCAAAAGGGTAGTATAGGTGCAAATGCTGTTATGACTGCTGCAAAAGCAGAAACCAAAACAGCAGAAAAAACAGCAACTTCTACCAAAGCTGCTTCAATAGAACTTAAAAAAACTGATACTGATATTAAGACTACAACTACTACCGAAAACAAATCAGCATCCGGATACAAACTAGATACCCTCTTTGGTGACTACATCTTGTGA", "aa": "MKLNLRFPSYFLPVVAASAFLVSCATPNTYEVQRAALIQLVEEDERQNYIQKGSIGANAVMTAAKAETKTAEKTATSTKAASIELKKTDTDIKTTTTTENKSASGYKLDTLFGDYIL", "aa_hexdigest": "2e8151c53336e0cecb2b29c36ad04f16", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12734.328199999978, "isoelectric_point": 7.770879173278809 }, "id": "CNOCLNGIAL_414", "locus": "GCA000143945_00379" }, { "type": "cds", "sequence": "contig_1", "start": 214685, "stop": 215203, "strand": "+", "frame": 2, "gene": null, "product": "MPN162 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75583", "UniRef:UniRef90_P75583" ], "nt": "GTGATTTTAGACGGTGGTAACTTGACCGTGGAAAAAAATCATGAACATGGCCATACTCATAAAAATGGTGAAACTCACGAACATGATCATGATCACCACGAAGGTGAAGAGGAAGTAATCGTTGGTCGTGCCCTTTCTTTTGCTAATGGTCTCTTCTTAGTTGTGGATCTTAAGGAAGAAAAACACGAAGAGAAGAAGGAAGCAAAATCTGAAATGTCAATGAACAGTAAAGATATGGTGATGATGACCAAAACTGAAATGATGTCTAAGGAAATGAAATCCGAACAAAAAATGGAGAAGAAGGAGGAACACGAACATCATCACAAAAAGCTGAGCCTCAGTACCACGGCTTACAAGTTTGGTCAAAGCTTTAACATCCTGGAATTTACTGGTGCTATGCACCACAAAACGGCACACAATAACGAAACCGAATTTAAAAATTTAGGCAAAAAATACGGTGGTATGACCGAGTACACGATTGTCGACTTTGACTTTAATCCACCAAAACCAACTAAGTAA", "aa": "MILDGGNLTVEKNHEHGHTHKNGETHEHDHDHHEGEEEVIVGRALSFANGLFLVVDLKEEKHEEKKEAKSEMSMNSKDMVMMTKTEMMSKEMKSEQKMEKKEEHEHHHKKLSLSTTAYKFGQSFNILEFTGAMHHKTAHNNETEFKNLGKKYGGMTEYTIVDFDFNPPKPTK", "aa_hexdigest": "431194463bd7e667d33c3c1996538570", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 214385, "stop": 215203, "observations": { "mutation": [ 214592 ], "stop": [ 214592 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75583", "query_cov": 1.0, "subject_cov": 0.5375, "identity": 0.9590000000000001, "score": 260.0, "evalue": 1.19e-84, "gene_start": 1, "gene_end": 172, "reference_start": 149, "reference_end": 320, "reference_sequence": "MKLNLRFPSYFLPVVAASAFLVSCATPNTYEVQRAALIQLVEEDERQNYIQKGSMGANAVMTAAKAETKTAEKTATSTKAASIELKKTDTDIKTTTTTENKSASGYKLDTLFGDYILWVVDHLSGLLFSPKTNNSTTTQKIQLITEDKMILDGGNLTVEKNHEHGHTHKNGETHEHDHDHHEGEEEVIVGRALSFANGLFLVVDLKEEKHEEKKEAKSEMSMNSKDMVMMTKTEMMSKEMKSEQKMEKKEEHEHPHKKLSLSTTAYKFGQSFNILEFTGAMHHKTAHNNETEFKNLGKKYGGMTEYTIVDFDFNPPKPTK" }, "paralog": false, "identity": 0.9632352941176471, "score": 410.0, "evalue": 5.67e-146, "description": "Internal stop codon at 214592. Nonsense mutation around 214592.", "psc": { "uniref90_id": "UniRef90_P75583", "product": "MPN162 family protein", "uniref50_id": "UniRef50_P75583", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75583", "UniRef:UniRef50_P75583", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75583", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75583" ], "product": "Uncharacterized lipoprotein MG149 homolog" } }, "id": "CNOCLNGIAL_415", "locus": "GCA000143945_00380" }, { "type": "cds", "sequence": "contig_1", "start": 215402, "stop": 215788, "strand": "+", "frame": 2, "gene": null, "product": "MPN163 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014574900.1", "SO:0001217", "UniParc:UPI0001DD64DA", "UniRef:UniRef100_A0A0H3DMB4", "UniRef:UniRef50_Q9ZB80", "UniRef:UniRef90_P75582" ], "nt": "TTGGAAAAAGATAAGGAATTAGTCAGTTCAATTGTGACTGCTAAAAGCATGATTGACCGGTTTTTCTGGTCTATTTTGAGCTTTCTGCTCACTAACTTAGTCTTTTTATTTGTGGCTTTTTTAATTCTGATTATTTACTTAATTTCCGAAATCACCCAACAGTTTGCCTTTGCTTTTATCGCAACGATTGTCTTCATCATTTTTTACAACATCCTCTTTTTGAGCTACCTGTTGACGATGTACATTAAAGGCTTAAAACAGATTGAGCAAAAGTCACGTTATTTACTCTTAGTGCTTGATGTTAAGGCTGATGAACTGCTTCCTTTCTCCTTTTTAGGTAGTTTAAGAAAGAGTCACATGCTTGAAGAAATGTTGTTAGAGCAGTAA", "aa": "MEKDKELVSSIVTAKSMIDRFFWSILSFLLTNLVFLFVAFLILIIYLISEITQQFAFAFIATIVFIIFYNILFLSYLLTMYIKGLKQIEQKSRYLLLVLDVKADELLPFSFLGSLRKSHMLEEMLLEQ", "aa_hexdigest": "a53a8e4bcd321239e32ee769b1a386f7", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64DA", "ncbi_nrp_id": "WP_014574900.1", "uniref100_id": "UniRef100_A0A0H3DMB4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64DA", "RefSeq:WP_014574900.1", "UniRef:UniRef100_A0A0H3DMB4" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DMB4", "uniref90_id": "UniRef90_P75582" }, "psc": { "uniref90_id": "UniRef90_P75582", "product": "MPN163 family protein", "uniref50_id": "UniRef50_Q9ZB80", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB80" ], "product": "Uncharacterized protein MG149.1" }, "genes": [], "id": "CNOCLNGIAL_416", "locus": "GCA000143945_00381" }, { "type": "cds", "sequence": "contig_1", "start": 215882, "stop": 216118, "strand": "+", "frame": 2, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627", "SO:0001217", "UniRef:UniRef50_O52331", "UniRef:UniRef90_P75581" ], "nt": "ATGAACGCCGCTAACGCAGTTAAATACCCTGAGCTCAAGATTAAGCTCGAGTCCTATGACAGCACCCTTTTAGACCTCACTACGAAAAAGATAGTGGAGGTAGTAAAGGGTGTCGATGTCAAAATTAAAGGTCCATTGCCTCTACCTACCAAAAAAGAGGTAATTACGATCATCCGTTCACCCCATGTGGATAAAGCATCACGCGAACAGTTTGAAAAAACCGCCACAAACGTTTAA", "aa": "MNAANAVKYPELKIKLESYDSTLLDLTTKKIVEVVKGVDVKIKGPLPLPTKKEVITIIRSPHVDKASREQFEKTATNV", "aa_hexdigest": "5e3975fb8bb61e9c69b74c12a7e2b399", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpsJ" ], "truncated": "3-prime", "pseudogene": { "start": 215882, "stop": 216207, "observations": { "deletion": [ 216098 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75581", "query_cov": 0.9358974358974359, "subject_cov": 0.6759259259259259, "identity": 1.0, "score": 142.0, "evalue": 2.17e-42, "gene_start": 1, "gene_end": 73, "reference_start": 1, "reference_end": 73, "reference_sequence": "MNAANAVKYPELKIKLESYDSTLLDLTTKKIVEVVKGVDVKIKGPLPLPTKKEVITIIRSPHVDKASREQFEKNRHKRLMILVDVNQGAIDSLKRIKIPVGVTLRFSK" }, "paralog": false, "identity": 1.0, "score": 204.0, "evalue": 2.14e-69, "description": "Frameshift due to deletion around 216098.", "psc": { "uniref90_id": "UniRef90_P75581", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_O52331", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75581", "UniRef:UniRef50_O52331", "COG:COG0051", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52331", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52331" ], "product": "Small ribosomal subunit protein uS10" } }, "id": "CNOCLNGIAL_417", "locus": "GCA000143945_00382" }, { "type": "cds", "sequence": "contig_1", "start": 216221, "stop": 217084, "strand": "+", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "RefSeq:WP_014574901.1", "SO:0001217", "UniParc:UPI0001DD64DB", "UniRef:UniRef100_A0AAX0SRM4", "UniRef:UniRef50_P75580", "UniRef:UniRef90_P75580" ], "nt": "ATGGAAATTCGTGGTATTTTTGGGGTTAAAGTAGGCATGAGCCAGGTGTTTACAACCAATAATGAGCGCCTACCTATTACCGTTATTTATTGTGAACCTAACCAGGTAGCAGGTGTTAAGAATGAGGCTAAGGACAAGTACAGTGCTACCTTGTTGAGTTTTGACACCGTTGAAAACAAAAAGCTCAACAAGCCTCAACAAGGCTTCTTTGAAAAAAACAACTTAAAGCCAACCAAGCACTTACAGGAAATCCGCAATATGACCGGGTTTGAAATGGGGCAACAAATCACACCGCAAAATCTCTTCCAAGTGGGTGAGTATGTTGATGTAAGTGCCATTAGTAAAGGTCGTGGGTTTACTGGTGCCATTAAGCGCTGGAACTTTAAGATCGGTCCGTTAGGTCATGGGGCTGGTTATCCCCACCGCTTCCAAGGTTCAGTCCAAGCTGGTCGTGGGGGTGCGAGTGCACAAAGAGTGTTTAAGGGCAAAAAGATGTCCGGACACTATGGTCATGAAAAAGTAACGGTACAAAACTTACGCATTGTTGGTTTTGATGAAGCTAACATGTTGGTATTAGTATCAGGGGCGATCGCAGGTCCTGAAGGTGGTGTGGTCTTCATCCGTACCGCTAAGAAAAAACCGGGGGTAGTGAAACCAATTGAGTTAGCTGTTCAAACCGAAAAGGCACCGGAAGCAAAACCGGCCAAGCTTTCCAAAAAGAAACAAGCCAAGGAGTTAGCGAAAGCACAAGCAGCTAACCAGCAAACTGTTGAAGCTAAGGTAGACACTCCAGTAGTAGAACCAAAACCAACTGAAGTTAAAAAAGCAGCACCAGTTGTAGAAAAGAAAGGGGAAGATAAATAA", "aa": "MEIRGIFGVKVGMSQVFTTNNERLPITVIYCEPNQVAGVKNEAKDKYSATLLSFDTVENKKLNKPQQGFFEKNNLKPTKHLQEIRNMTGFEMGQQITPQNLFQVGEYVDVSAISKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGASAQRVFKGKKMSGHYGHEKVTVQNLRIVGFDEANMLVLVSGAIAGPEGGVVFIRTAKKKPGVVKPIELAVQTEKAPEAKPAKLSKKKQAKELAKAQAANQQTVEAKVDTPVVEPKPTEVKKAAPVVEKKGEDK", "aa_hexdigest": "e795c047f8e1f3d97d7962546f601e22", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64DB", "ncbi_nrp_id": "WP_014574901.1", "uniref100_id": "UniRef100_A0AAX0SRM4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64DB", "RefSeq:WP_014574901.1", "UniRef:UniRef100_A0AAX0SRM4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SRM4", "uniref90_id": "UniRef90_P75580" }, "psc": { "uniref90_id": "UniRef90_P75580", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_P75580", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75580", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75580" ], "product": "Large ribosomal subunit protein uL3" }, "genes": [ "rplC" ], "id": "CNOCLNGIAL_418", "locus": "GCA000143945_00383" }, { "type": "cds", "sequence": "contig_1", "start": 217084, "stop": 217218, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAGCTTAAACTAATTAAAATCGACGGTTCCTTTGAAACTGAACCAGTGAAACTAAGCCCTGGTTTAATTGCTAAGGAACTCAAACAACAACCGGTGTTTGATGCCGTGTTAGTAGAACAAGCTTCCTGA", "aa": "MAKLKLIKIDGSFETEPVKLSPGLIAKELKQQPVFDAVLVEQAS", "aa_hexdigest": "606d57c287ba36a74d465242d00bebc1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4810.649100000002, "isoelectric_point": 6.090491294860839 }, "id": "CNOCLNGIAL_419", "locus": "GCA000143945_00384" }, { "type": "cds", "sequence": "contig_1", "start": 217351, "stop": 217722, "strand": "+", "frame": 1, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:0046677", "GO:1990904", "SO:0001217", "UniRef:UniRef50_P75579", "UniRef:UniRef90_P75579" ], "nt": "GTGTTTGGTCCCAAGCCCAATCGTAACTACAGCTTAAAACTCAACAAGAAAGCGCACACGGCAGCCTTGCACACTGTTTGGAGTGAAAAGTTAGCTTCAGACAACACCCACCTAGTGGACCAAAACCTCTTTAACAAAACTGAGGGTAAAACCAAGGTGATGGTGCAATTTTTAAAGAGTGCCAAGCTCTTGGACAAAAACGTGTTGTTTGTAGTTAATACCCTTAACACCAACTTAGAACAAAGCACCAGCAACATTAAAAACGTGCAGGTCAAACACCTAGACAAGGTTTCTGTAAGGGATCTGATGTTAGCTAACGCTCTTTTAGTTGAAAAAGAAGTTTTAAAAGCTTTGGAGGGCAAATTTAAATAA", "aa": "MFGPKPNRNYSLKLNKKAHTAALHTVWSEKLASDNTHLVDQNLFNKTEGKTKVMVQFLKSAKLLDKNVLFVVNTLNTNLEQSTSNIKNVQVKHLDKVSVRDLMLANALLVEKEVLKALEGKFK", "aa_hexdigest": "d8daaef8ac53e53dfb3e1f423f3bea66", "start_type": "GTG", "rbs_motif": null, "genes": [ "rplD" ], "truncated": "5-prime", "pseudogene": { "start": 217084, "stop": 217722, "observations": { "mutation": [ 217216 ], "stop": [ 217216 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75579", "query_cov": 1.0, "subject_cov": 0.5801886792452831, "identity": 0.9840000000000001, "score": 234.0, "evalue": 1.08e-76, "gene_start": 1, "gene_end": 123, "reference_start": 90, "reference_end": 212, "reference_sequence": "MAKLKLIKIDGSFETEPVKLSPGLIAKELKQQPVFDAVLVEQASWRQGTHSILTKGEVRGGGKKPYKQKHTGKARQGSTRNPHFVGGGIVFGPKPNRNYSLKLNKKAHTAALHTVWSEKLASDNTHLVDQNLFNKTEGKTKVMMQFLKSAKLLDKNVLFVVNTLNTNLEQSTSNIKNVQVKHLDKVSVRDLMLANALLVEKEVLKALEGKFK" }, "paralog": false, "identity": 0.9905660377358491, "score": 412.0, "evalue": 2.34e-149, "description": "Internal stop codon at 217216. Nonsense mutation around 217216.", "psc": { "uniref90_id": "UniRef90_P75579", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_P75579", "cog_id": "COG0088", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:0046677", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75579", "UniRef:UniRef50_P75579", "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:0046677", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P75579", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75579" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "CNOCLNGIAL_420", "locus": "GCA000143945_00385" }, { "type": "cds", "sequence": "contig_1", "start": 217725, "stop": 218438, "strand": "+", "frame": 3, "gene": "rplW", "product": "Large ribosomal subunit protein uL23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874524.1", "SO:0001217", "UniParc:UPI0000133DA4", "UniRef:UniRef100_P75578", "UniRef:UniRef50_P75578", "UniRef:UniRef90_P75578" ], "nt": "ATGGATGTAACGAACGTACTGCTAAAACCAGTTCTAACCGAAAAGGTGTACTTCAACCAAATGGGTGAAACCAAGAAGTACGTCTTTGTGGTGAACCCTAAAGCAAGTAAAACACGCGTTAAACTAGCTTTTGAATTAGTGTATGGCATTAAGCCACTGAAGGTTAACACCTTAATTAGAAAACCAACCACCATTCGCGGTGGGAGCCGTTTTCCTGGTTTGAGCAAACTAGAAAAGTTGGCTGTGATTACCTTACCAAAGGGTATTGCCATTTCAGTTACTGGTGAAGCGCCAGAAAAAACCGATAAGCCAGCGGACAAAACCACCCTTAAGGAAAGTACTGTTAAGGAGATTAAGGACACCAAAAACAGTCCAGAAGCTGTTGTGAAAACTGCTGTGGAAGCGTTGCAAATTAAACCAACTGCCGCTCCTGTAACCACCGCCCCTCTACAAACCGTGGCGGTGAAGGTAGCTAAAGAAGTTAAAGAAGTTAAGGTAGAAAAGCCGGTTAAAGTGGAAAAACCAACCAAACCGGCAAAGGTAGCAAAAGAAGCTAAAACGACCAAAGTAGCTAAGGAAACTAAAGCTGAAAAGTCAGTTCAAACAACCAAAGTAGCTAAGGAAACTAAAACTGAAAAGTCAGCTAAAACTACCAAAACTACAGCTACAAAAACTACTAAAACTAAAACCACCAAAAAGGAGGTTAAAAAGTAA", "aa": "MDVTNVLLKPVLTEKVYFNQMGETKKYVFVVNPKASKTRVKLAFELVYGIKPLKVNTLIRKPTTIRGGSRFPGLSKLEKLAVITLPKGIAISVTGEAPEKTDKPADKTTLKESTVKEIKDTKNSPEAVVKTAVEALQIKPTAAPVTTAPLQTVAVKVAKEVKEVKVEKPVKVEKPTKPAKVAKEAKTTKVAKETKAEKSVQTTKVAKETKTEKSAKTTKTTATKTTKTKTTKKEVKK", "aa_hexdigest": "d08555cd895ec3b00883a94da7e8e52e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DA4", "ncbi_nrp_id": "WP_010874524.1", "uniref100_id": "UniRef100_P75578", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DA4", "RefSeq:WP_010874524.1", "UniRef:UniRef100_P75578" ] }, "ips": { "uniref100_id": "UniRef100_P75578", "uniref90_id": "UniRef90_P75578" }, "psc": { "uniref90_id": "UniRef90_P75578", "gene": "rplW", "product": "Large ribosomal subunit protein uL23", "uniref50_id": "UniRef50_P75578", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:1990904", "GO:0005840", "GO:0019843", "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P75578", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75578" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "CNOCLNGIAL_421", "locus": "GCA000143945_00386" }, { "type": "cds", "sequence": "contig_1", "start": 218438, "stop": 219301, "strand": "+", "frame": 2, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "COG:COG0090", "COG:J", "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843", "KEGG:K02886", "RefSeq:WP_010874525.1", "SO:0001217", "UniParc:UPI0000133ECF", "UniRef:UniRef100_P75577", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_P75577" ], "nt": "ATGCCTATTAAGAAAATTATTAGTCGTTCTAACAGTGGGATCCACCACTCCACGGTAATTGACTATAAGAAGTTATTAACCACCAATAAGAACAAACCAGAAAAGTCGTTGTTAGTTACCCTTAAAAAACACGGGGGTCGCAACAACCAAGGGAAAATTACGGTGCGCCACCAGGGTGGACGCAACAAGCGCAAGTACCGCATTATTGACTTTAAACGAACGCACTATGACAACATCGAAGCGACCGTTAAGTCGATCGAGTACGATCCGAACCGTTCTTGCTTTGTGTCGTTAATTACTTACGCTAACGGTGCCAAGAGTTACATCATTAGCCCTGATGGCATTAAGGTAGGTGATAAGATCTTGGCTTCCGAACACCCGATTGACATTAAACCGGGCTTTAGTATGCCCTTAGCCTTTATCCCTGAAGGTACCCAAGTGCATAACATTGAACTCCACCCTAAGGGAGGTGGTCAAATTGCCAGGAGTGCGGGGAGTTATGCCCGCATTTTGGGTCAAGACGAAACTGGTAAGTATGTCATTTTGCAACTTTTAAGTGGTGAAACACGCAAGTTCTTAAAGGAGTGCCGTGCTACTGTGGGAGTAGTTTCTAACTTAGACCACAACCTCGTAGTCATTGGTAAGGCTGGTCGCAACCGTCACCGCGGCATCCGTCCAACCGTTCGTGGTTCGGCCATGAACCCCAATGACCACCCCCACGGTGGTGGTGAAGGTCGTAGTCCCGTGGGTCGGGATGCACCCCGGACTCCATGGGGTAAGCGCCACATGGGTGTGAAGACCCGCAACATGAAGAAAGCATCCACTAACTTAATTATTCGTAACCGGAAAGGAGAACAATACTAA", "aa": "MPIKKIISRSNSGIHHSTVIDYKKLLTTNKNKPEKSLLVTLKKHGGRNNQGKITVRHQGGRNKRKYRIIDFKRTHYDNIEATVKSIEYDPNRSCFVSLITYANGAKSYIISPDGIKVGDKILASEHPIDIKPGFSMPLAFIPEGTQVHNIELHPKGGGQIARSAGSYARILGQDETGKYVILQLLSGETRKFLKECRATVGVVSNLDHNLVVIGKAGRNRHRGIRPTVRGSAMNPNDHPHGGGEGRSPVGRDAPRTPWGKRHMGVKTRNMKKASTNLIIRNRKGEQY", "aa_hexdigest": "84ec1e77c99929a1da5f4d6fe0e5aad7", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0000133ECF", "ncbi_nrp_id": "WP_010874525.1", "uniref100_id": "UniRef100_P75577", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133ECF", "RefSeq:WP_010874525.1", "UniRef:UniRef100_P75577" ] }, "ips": { "uniref100_id": "UniRef100_P75577", "uniref90_id": "UniRef90_P75577" }, "psc": { "uniref90_id": "UniRef90_P75577", "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "cog_id": "COG0090", "cog_category": "J", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "CNOCLNGIAL_422", "locus": "GCA000143945_00387" }, { "type": "cds", "sequence": "contig_1", "start": 219301, "stop": 219564, "strand": "+", "frame": 1, "gene": "rpsS", "product": "30S ribosomal protein S19", "db_xrefs": [ "COG:COG0185", "COG:J", "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "RefSeq:WP_010874526.1", "SO:0001217", "UniParc:UPI0000134DEC", "UniRef:UniRef100_P75576", "UniRef:UniRef50_Q6ME59", "UniRef:UniRef90_P75576" ], "nt": "ATGTCACGCAGTGCTAAAAAAGGAGCCTTTGTTGATGCTCACCTCTTAAAGAAAGTGATCGATATGAACAAACAAGAGAAAAAACGTCCGATCAAAACCTGGTCCCGTCGTAGTACCATCTTCCCCGAATTTGTCGGTAATACCTTTGCTGTACATAACGGCAAGACCTTCATTAATGTTTATGTAACTGATGACATGGTGGGGCACAAACTCGGTGAGTTCTCCCCGACCCGTAACTTCAAACAACACACCGCTAACCGTTAG", "aa": "MSRSAKKGAFVDAHLLKKVIDMNKQEKKRPIKTWSRRSTIFPEFVGNTFAVHNGKTFINVYVTDDMVGHKLGEFSPTRNFKQHTANR", "aa_hexdigest": "9fa790bb912197d37c1ffb6630f98a3c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134DEC", "ncbi_nrp_id": "WP_010874526.1", "uniref100_id": "UniRef100_P75576", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DEC", "RefSeq:WP_010874526.1", "UniRef:UniRef100_P75576" ] }, "ips": { "uniref100_id": "UniRef100_P75576", "uniref90_id": "UniRef90_P75576" }, "psc": { "uniref90_id": "UniRef90_P75576", "gene": "rpsS", "product": "30S ribosomal protein S19", "uniref50_id": "UniRef50_Q6ME59", "cog_id": "COG0185", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q6ME59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6ME59" ], "product": "Small ribosomal subunit protein uS19" }, "genes": [ "rpsS" ], "id": "CNOCLNGIAL_423", "locus": "GCA000143945_00388" }, { "type": "cds", "sequence": "contig_1", "start": 219566, "stop": 220045, "strand": "+", "frame": 2, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "GO:0046677", "RefSeq:WP_014574902.1", "SO:0001217", "UniParc:UPI0001DD64DD", "UniRef:UniRef100_A0AAP8JFH9", "UniRef:UniRef50_P75575", "UniRef:UniRef90_P75575" ], "nt": "ATGATCGCCTTTGCAAAACAATTCCGTGTGAGAATCTCACCCCAAAAAGCCCGCTTAGTGTGTCAACTCATTGTGGGTAAGAAAACCGCTGATGCCCAAAACATCCTTTCCAATACACCCAAAAAAGCGGCTACCTTAATCGCTAAACTACTCAACAGTGCGATTGCCAACGCTACCAATAACCACGGCATGAATGGGGATGCCCTTTATGTCTTTGAGTGTGTGGCTAACCAAGGTCCGAGTATGAAGCGCACTATTCCCCGCGCTAAGGGCTCGAGTAACATGATTACCAAGCGCTCTTCTAATTTAGTGGTGAAACTATCGGATAACCCGAATGAACGTCAAGAATTAATTAAACAACAAAAGGCCCTAGTTAAAAAACGGGTCGAAGGTCAACAAAAAGCAAAGATGGCACGTCAAAAAGCAGTAACACCAGTGGTAAAAGCGCCAAGTAAAACTCAAGGAGGTGTACAAAAGTAA", "aa": "MIAFAKQFRVRISPQKARLVCQLIVGKKTADAQNILSNTPKKAATLIAKLLNSAIANATNNHGMNGDALYVFECVANQGPSMKRTIPRAKGSSNMITKRSSNLVVKLSDNPNERQELIKQQKALVKKRVEGQQKAKMARQKAVTPVVKAPSKTQGGVQK", "aa_hexdigest": "d2a87f364f2e381fc97661d6c0982c33", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64DD", "ncbi_nrp_id": "WP_014574902.1", "uniref100_id": "UniRef100_A0AAP8JFH9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64DD", "RefSeq:WP_014574902.1", "UniRef:UniRef100_A0AAP8JFH9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8JFH9", "uniref90_id": "UniRef90_P75575" }, "psc": { "uniref90_id": "UniRef90_P75575", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_P75575", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "GO:0046677" ] }, "pscc": { "uniref50_id": "UniRef50_P75575", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75575" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "CNOCLNGIAL_424", "locus": "GCA000143945_00389" }, { "type": "cds", "sequence": "contig_1", "start": 220212, "stop": 220868, "strand": "+", "frame": 3, "gene": "rpsC", "product": "30S ribosomal protein S3", "db_xrefs": [ "COG:COG0092", "COG:J", "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627", "KEGG:K02982", "SO:0001217", "UniRef:UniRef50_O52338", "UniRef:UniRef90_P41205" ], "nt": "GTGGAGATTGAACGTACTCAAGCAACCGTGGACGTATTTGTGTACGCCGCCCAACCCGCTTTTCTCATTGGTAGTGAAAACAAGAACATCCAAAAGATCACCAAGCAAATTAAGCAAATTATTGGTCGTACCACCAATTTAGACCTCACCATTAACGAAATTGGCTCCCCTATGTTATCAGCGCGCATTATTGCGCGGGATCTAGCCAACGCGATTGAAGCACGGGTACCGTTACGAACGGCAATGCGCCAATCCTTAATTAAGGTGTTGAAGGCTGGCGCTAACGGGATTAAGGTGTTGGTATCGGGTCGGTTAAACGGTGCCGAAATTGCGCGCGACAAGATGTACATCGAGGGCAATATGCCACTGTCAACCCTGCGTGCTGACATTGACTATGCCTTGGAAAAAGCGCAAACTACGTACGGTGTCATTGGTGTTAAGGTATGGATTAATCGCGGCATGATTTACACCAAGGGCTTAAACCGCACCCCGGCCCACATTCTCCACCCACAAAAGAAACAACCAAACCGCCAAAACCAACAACCACGCCACTTTAACCAGGGCCAGGTGTTGAGTGCTAATAAGTTAACTGGTAGTGATGTGGAGACCAGCAGTATCCAAGCACTTACTAAGCCTAACAAGGAGGACAAACAATAA", "aa": "MEIERTQATVDVFVYAAQPAFLIGSENKNIQKITKQIKQIIGRTTNLDLTINEIGSPMLSARIIARDLANAIEARVPLRTAMRQSLIKVLKAGANGIKVLVSGRLNGAEIARDKMYIEGNMPLSTLRADIDYALEKAQTTYGVIGVKVWINRGMIYTKGLNRTPAHILHPQKKQPNRQNQQPRHFNQGQVLSANKLTGSDVETSSIQALTKPNKEDKQ", "aa_hexdigest": "93133787fde845f73905639b469b8ff7", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpsC" ], "truncated": "5-prime", "pseudogene": { "start": 220047, "stop": 220868, "observations": { "mutation": [ 220110 ], "stop": [ 220110 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P41205", "query_cov": 1.0, "subject_cov": 0.7985347985347986, "identity": 0.995, "score": 413.0, "evalue": 6.56e-145, "gene_start": 1, "gene_end": 218, "reference_start": 56, "reference_end": 273, "reference_sequence": "MGQKVNSNGLRFGINKNWISRWTANSHAQTAKWLIEDEKIRNLFFVNYRNAQVSNVEIERTQATVDVFVYAAQPAFLIGSENKNIQKITKQIKQIIGRTTNLDLTINEIGSPMLSARIIARDLANAIEARVPLRTAMRQSLIKVLKAGANGIKVLVSGRLNGAEIARDKMYIEGNMPLSTLRADIDYALEKAQTTYGVIGVKVWINRGMIYTKGLNRTPAHILHPQKKQPNRQNQQPRHFNQGQVLSANKLTGSDVETSSIQALTKPNKEDKQ" }, "paralog": false, "identity": 0.9963369963369964, "score": 532.0, "evalue": 3.69e-194, "description": "Internal stop codon at 220110. Nonsense mutation around 220110.", "psc": { "uniref90_id": "UniRef90_P41205", "gene": "rpsC", "product": "30S ribosomal protein S3", "uniref50_id": "UniRef50_O52338", "cog_id": "COG0092", "cog_category": "J", "kegg_orthology_id": "K02982", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P41205", "UniRef:UniRef50_O52338", "COG:COG0092", "COG:J", "KEGG:K02982", "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52338" ], "product": "Small ribosomal subunit protein uS3" } }, "id": "CNOCLNGIAL_425", "locus": "GCA000143945_00390" }, { "type": "cds", "sequence": "contig_1", "start": 220868, "stop": 221254, "strand": "+", "frame": 2, "gene": "rplP", "product": "50S ribosomal protein L16", "db_xrefs": [ "COG:COG0197", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02878", "SO:0001217", "UniRef:UniRef50_P02415", "UniRef:UniRef90_P47404" ], "nt": "ATGTTACAACCAAAAAGAACAAAGTACCGCAAACCTCACAACGTGAGTTACGAGGGCAAGGCTAAGGGCAACAGCTATGTTGCTTTTGGTGAATATGGTCTAGTAGCTACTAAGGGTAACTGGATCGATGCCCGTGCCATAGAATCAGCCCGGATTGCCATTAGTAAGTGTTTGGGTAAAACCGGTAAGATGTGGATCCGCATCTTCCCCCACATGTCCAAGACTAAAAAACCGTTAGAAGTACGGATGGGTTCTGGTAAGGGTAACCCGGAATTCTGGGTAGCAGTAGTAAAACAGGGCACGGTGATGTTTGAGGTAGCTAACATCCCTGAAAGCCAAATGATTAAGGCTTTAACCCGTGCTGGTCACAAGTTACCCGTGACCTGA", "aa": "MLQPKRTKYRKPHNVSYEGKAKGNSYVAFGEYGLVATKGNWIDARAIESARIAISKCLGKTGKMWIRIFPHMSKTKKPLEVRMGSGKGNPEFWVAVVKQGTVMFEVANIPESQMIKALTRAGHKLPVT", "aa_hexdigest": "a065ea1cc9e1d1d733ac3d40561c3b20", "start_type": "ATG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_P47404", "query_cov": 1.0, "subject_cov": 0.920863309352518, "identity": 1.0, "score": 262.0, "evalue": 1.23e-88, "valid": true, "gene": "rplP", "product": "50S ribosomal protein L16", "uniref50_id": "UniRef50_P02415", "cog_id": "COG0197", "cog_category": "J", "kegg_orthology_id": "K02878", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P02415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P02415" ], "product": "Large ribosomal subunit protein uL16" }, "genes": [ "rplP" ], "id": "CNOCLNGIAL_426", "locus": "GCA000143945_00391" }, { "type": "cds", "sequence": "contig_1", "start": 221287, "stop": 221481, "strand": "+", "frame": 1, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "SO:0001217", "UniRef:UniRef50_Q50310", "UniRef:UniRef90_Q50310" ], "nt": "ATGACCGTTGCAAAAGAATTACGCCAAAAGAGTTCCGAAGAGTTGGTAAAACTCGTGATTAAGCTCAAAGGGGAACTGTTAGAGTACCGCTTTAAGTTAGCACACGGGGAGCTAGACAAACCGCACTTAATTAACCAAACCAGGCGGTTATTGGCTACCATTTTAACGATTCTTACCGAGCGTAAGTTGAACTGA", "aa": "MTVAKELRQKSSEELVKLVIKLKGELLEYRFKLAHGELDKPHLINQTRRLLATILTILTERKLN", "aa_hexdigest": "3c1ca8354d0101d23fdc06853121825e", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpmC" ], "truncated": "3-prime", "pseudogene": { "start": 221287, "stop": 221622, "observations": { "mutation": [ 221479 ], "stop": [ 221479 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50310", "query_cov": 1.0, "subject_cov": 0.5765765765765766, "identity": 1.0, "score": 119.0, "evalue": 9.11e-34, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MTVAKELRQKSSEELVKLVIKLKGELLEYRFKLAHGELDKPHLINQTRRLLATILTILTERKLNWQEEQAKYKLLTKKTNEAAVNAWKQHLEANKAKLLKSRAKREDASKK" }, "paralog": false, "identity": 0.990990990990991, "score": 210.0, "evalue": 5.49e-72, "description": "Internal stop codon at 221479. Nonsense mutation around 221479.", "psc": { "uniref90_id": "UniRef90_Q50310", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_Q50310", "cog_id": "COG0255", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50310", "UniRef:UniRef50_Q50310", "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q50310", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50310" ], "product": "Large ribosomal subunit protein uL29" } }, "id": "CNOCLNGIAL_427", "locus": "GCA000143945_00392" }, { "type": "cds", "sequence": "contig_1", "start": 221622, "stop": 221879, "strand": "+", "frame": 3, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_014574903.1", "SO:0001217", "UniParc:UPI0001DD64DE", "UniRef:UniRef100_A0AB38W943", "UniRef:UniRef50_P47406", "UniRef:UniRef90_Q50309" ], "nt": "ATGAAACGTAATCAAAGAAAGGTCCTAATTGGGATTGTTAAAAGTACTAAAAACGCCAAGACCGCTACGGTTCAAGTAGAATCACGGTTTAAGCACCCTTTGTACCATAAGTCCGTAGTGCGACACAAGAAGTACCAAGCCCACAATGAGGGTGAAGTACTCGCTAAGGACGGGGACAAGGTACAAATTGTGGAAACGCGACCGTTGTCTGCAACCAAGCGCTTCCGCATTGCCAAAATTATTGAGAGGGTTAAATAG", "aa": "MKRNQRKVLIGIVKSTKNAKTATVQVESRFKHPLYHKSVVRHKKYQAHNEGEVLAKDGDKVQIVETRPLSATKRFRIAKIIERVK", "aa_hexdigest": "c55f90206a460526b4173d3df13a12bf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64DE", "ncbi_nrp_id": "WP_014574903.1", "uniref100_id": "UniRef100_A0AB38W943", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64DE", "RefSeq:WP_014574903.1", "UniRef:UniRef100_A0AB38W943" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W943", "uniref90_id": "UniRef90_Q50309" }, "psc": { "uniref90_id": "UniRef90_Q50309", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_P47406", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47406" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "CNOCLNGIAL_428", "locus": "GCA000143945_00393" }, { "type": "cds", "sequence": "contig_1", "start": 221895, "stop": 222251, "strand": "+", "frame": 3, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02874", "SO:0001217", "UniRef:UniRef50_Q5SHP8", "UniRef:UniRef90_P47407" ], "nt": "ATGACAAGACTAAATGTTGCCGATAACACGGGCGCCAAGCAAGTGGGCATCATTAAGGTATTAGGTTCCACCCGGAAACGTTATGCCTTTTTGGGTGATGTGGTAGTGGTATCAGTTAAGGATGCTATCCCTAGCGGTATGGTCAAAAAGGGTCAAGTGCTACGGGCAGTAATTGTCCGCACTAAAAAGGGCCAACAACGTAAGGATGGTACCCACCTTAAGTTTGATGACAACGCTTGTGTGTTAATTAAGGAAGACAAGTCACCAAGAGGCACACGGATCTTTGGTCCGGTAGCGCGTGAACTACGGGAACGTGGTTACAACAAAATTTTGAGTTTAGCAGTGGAGGTAGTGTAA", "aa": "MTRLNVADNTGAKQVGIIKVLGSTRKRYAFLGDVVVVSVKDAIPSGMVKKGQVLRAVIVRTKKGQQRKDGTHLKFDDNACVLIKEDKSPRGTRIFGPVARELRERGYNKILSLAVEVV", "aa_hexdigest": "1d4d34751b9f8d42d5606a6c0bb09451", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47407", "query_cov": 1.0, "subject_cov": 0.9672131147540983, "identity": 0.9490000000000001, "score": 220.0, "evalue": 1.37e-72, "valid": true, "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_Q5SHP8", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHP8" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "CNOCLNGIAL_429", "locus": "GCA000143945_00394" }, { "type": "cds", "sequence": "contig_1", "start": 222251, "stop": 222586, "strand": "+", "frame": 2, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874533.1", "SO:0001217", "UniParc:UPI0000133DCD", "UniRef:UniRef100_Q50307", "UniRef:UniRef50_O52343", "UniRef:UniRef90_Q50307" ], "nt": "ATGCAACGAATTAAAAAAGGTGATAAAGTAGTCGTAATTACCGGTAAGAATAAGGGTGGTAGTGGCATTGTTTTGAAAATTATGCCCGCGCGCCAACAAGCAATTGTAGAGGGACTCAACAAAGTAACACGCCACAAAAAGAAGGACCAAACCACAAAGCGCGCTGCTAAACAATCGACCGGTAAAGTGCAACAAGAAGCGCCAATCTTCTTGAGCAAGTTAGCTTTATTTGACCAAAAGGCAAAACAACAAACAATTGGCAAAATTAAGTACGTTATGGACCCTAAGACTAATAAGAAGACCCGGGTCTTTAAGAAATCCAACAACACGCTCTAG", "aa": "MQRIKKGDKVVVITGKNKGGSGIVLKIMPARQQAIVEGLNKVTRHKKKDQTTKRAAKQSTGKVQQEAPIFLSKLALFDQKAKQQTIGKIKYVMDPKTNKKTRVFKKSNNTL", "aa_hexdigest": "b672114d9ef8bdd0628ab567967334f1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DCD", "ncbi_nrp_id": "WP_010874533.1", "uniref100_id": "UniRef100_Q50307", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DCD", "RefSeq:WP_010874533.1", "UniRef:UniRef100_Q50307" ] }, "ips": { "uniref100_id": "UniRef100_Q50307", "uniref90_id": "UniRef90_Q50307" }, "psc": { "uniref90_id": "UniRef90_Q50307", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_O52343", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_O52343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52343" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "CNOCLNGIAL_430", "locus": "GCA000143945_00395" }, { "type": "cds", "sequence": "contig_1", "start": 222589, "stop": 223131, "strand": "+", "frame": 1, "gene": "rplE", "product": "50S ribosomal protein L5", "db_xrefs": [ "COG:COG0094", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02931", "RefSeq:WP_014574904.1", "SO:0001217", "UniParc:UPI0001DD64DF", "UniRef:UniRef100_A0AAX0S400", "UniRef:UniRef50_Q50306", "UniRef:UniRef90_Q50306" ], "nt": "ATGAATAATCTCAAAGCACATTACCAAAAAACAATTGCCAAAGAGCTGCAAAAGAGCTTTGCGTTTTCCTCGATTATGCAAGTACCGCGCTTGGAAAAAATTGTGATTAACATGGGGGTGGGTGACGTCATTCGCGACAGTAAGTTCTTGGAATCAGCCTTAAACGAACTCCACCTCATTAGCGGTCAAAAACCAGTTGCTACTAAGGCTAAAAACGCCATTTCCACCTATAAGCTGCGCGCTGGTCAACTAATCGGTTGTAAGGTTACCTTGCGCGGTGAGCGGATGTGGGCCTTTTTGGAAAAACTGATTTATGTTGCCCTACCTCGGGTGCGGGATTTCCGCGGCCTTTCTTTAAAGTCTTTTGATGGTCGGGGTAACTATACCATTGGAATTAAAGAACAAATTATTTTCCCGGAAATTGTCTATGACGACATTAAACGGATCCGCGGGTTTGACGTTACTCTGGTAACTTCAACCAATAAGGACAGCGAAGCATTAGCTTTACTTAGAGCGTTAAACCTCCCACTTGTAAAAGGATAA", "aa": "MNNLKAHYQKTIAKELQKSFAFSSIMQVPRLEKIVINMGVGDVIRDSKFLESALNELHLISGQKPVATKAKNAISTYKLRAGQLIGCKVTLRGERMWAFLEKLIYVALPRVRDFRGLSLKSFDGRGNYTIGIKEQIIFPEIVYDDIKRIRGFDVTLVTSTNKDSEALALLRALNLPLVKG", "aa_hexdigest": "545129f351792f8f81d9277d60aea8bf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64DF", "ncbi_nrp_id": "WP_014574904.1", "uniref100_id": "UniRef100_A0AAX0S400", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64DF", "RefSeq:WP_014574904.1", "UniRef:UniRef100_A0AAX0S400" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S400", "uniref90_id": "UniRef90_Q50306" }, "psc": { "uniref90_id": "UniRef90_Q50306", "gene": "rplE", "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_Q50306", "cog_id": "COG0094", "cog_category": "J", "kegg_orthology_id": "K02931", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q50306", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50306" ], "product": "Large ribosomal subunit protein uL5" }, "genes": [ "rplE" ], "id": "CNOCLNGIAL_431", "locus": "GCA000143945_00396" }, { "type": "cds", "sequence": "contig_1", "start": 223133, "stop": 223315, "strand": "+", "frame": 2, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "COG:COG0199", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_P12878", "UniRef:UniRef90_P47410" ], "nt": "ATGGCCAAAAAATCATTAAAAGTAAAACAAACTAGAATCCCCAAATTTGCCGTACGGGCTTACACCCGCTGTCAGCGTTGTGGTCGAGCCCGGGCAGTTTTAAGCCACTTTGGTGTGTGCCGTTTGTGCTTCCGCGAACTAGCATACGCTGGCGCAATTCCAGGAGTTAAAAAAGCATCATGA", "aa": "MAKKSLKVKQTRIPKFAVRAYTRCQRCGRARAVLSHFGVCRLCFRELAYAGAIPGVKKAS", "aa_hexdigest": "e4be5aeb126691b9d760f7afa251758a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47410", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 0.9, "score": 103.0, "evalue": 3.34e-28, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_P12878", "cog_id": "COG0199", "cog_category": "J", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P12878", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P12878" ], "product": "Small ribosomal subunit protein uS14B" }, "genes": [ "rpsZ" ], "id": "CNOCLNGIAL_432", "locus": "GCA000143945_00397" }, { "type": "cds", "sequence": "contig_1", "start": 223312, "stop": 223740, "strand": "+", "frame": 1, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874536.1", "SO:0001217", "UniParc:UPI000013516F", "UniRef:UniRef100_Q50304", "UniRef:UniRef50_Q9PQP6", "UniRef:UniRef90_Q50304" ], "nt": "ATGATAACAACTACAAAACCAATTAAAGCCCACTTTGATCCAGTGGCTGACTTACTAACGAAGATTAACAATGCGCGCAAAGCGAAGTTAATGACGGTAACCACAATTGCTTCCAAGCTCAAGATTGCCATCTTGGAAATTTTGGTCAAGGAAGGTTATTTAGCTAACTTCCAGGTCTTGGAAAACAAGAGCAAGACCAAGCGGATTGTCACCTTTAACTTAAAATACACGCAGCGCCGGATTCCCTCCATTAACGGGGTGAAACAAATTTCCAAGCCGGGTCTGCGGATCTACCGGCCCTTTGAAAAGCTCCCGTTAGTTTTAAACGGTCTTGGGATTGCCATTATTTCCACTAGTGATGGCGTGATGACTGATAAGGTAGCGCGCTTAAAGAAAATTGGAGGGGAGATTCTCGCTTACGTTTGGTAA", "aa": "MITTTKPIKAHFDPVADLLTKINNARKAKLMTVTTIASKLKIAILEILVKEGYLANFQVLENKSKTKRIVTFNLKYTQRRIPSINGVKQISKPGLRIYRPFEKLPLVLNGLGIAIISTSDGVMTDKVARLKKIGGEILAYVW", "aa_hexdigest": "7d47000330783529090b357afb242771", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013516F", "ncbi_nrp_id": "WP_010874536.1", "uniref100_id": "UniRef100_Q50304", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013516F", "RefSeq:WP_010874536.1", "UniRef:UniRef100_Q50304" ] }, "ips": { "uniref100_id": "UniRef100_Q50304", "uniref90_id": "UniRef90_Q50304" }, "psc": { "uniref90_id": "UniRef90_Q50304", "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q9PQP6", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP6" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "CNOCLNGIAL_433", "locus": "GCA000143945_00398" }, { "type": "cds", "sequence": "contig_1", "start": 223747, "stop": 224208, "strand": "+", "frame": 1, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "SO:0001217", "UniRef:UniRef50_P47412", "UniRef:UniRef90_Q50303" ], "nt": "ATGTCAAAAATAGGAAACAGAACGATTACACTAGACCCCGCTAAGGTCAACCTTAACTTCCAGAAGGACCACATTGCCGTTAAGGGTCCTTTGGGTCAAATTGAGCTCAAACTACCCCCTAACTTACCGCTCAAGTTTGAACTGAAGGATAATAACCTCCAAATTACGCGGAACAACGAGTTAAAGCAATCCAAGATCTTCCATGGTACTTACAACGCGTTAATTACCAACGCCATTATTGGGGTTACTCAAGGCTTTGAAAAGAAGCTGCGCTTAGTCGGTGTGGGTTACCGTGCTAACGTGGAAGGGGAAACGCTCAACCTCCAGTTGGGTTACTCCCATCCCATTAAGGAAAAGATTCCCAAAGGCTTAACAGTTAAAGTAGAAAAGAACACCGAGATTACCATTTCGGGAATTAGTAAGGAGTTAGTGGGTCAGTTTGCCACTGAGGTGCGCAAGTGA", "aa": "MSKIGNRTITLDPAKVNLNFQKDHIAVKGPLGQIELKLPPNLPLKFELKDNNLQITRNNELKQSKIFHGTYNALITNAIIGVTQGFEKKLRLVGVGYRANVEGETLNLQLGYSHPIKEKIPKGLTVKVEKNTEITISGISKELVGQFATEVRK", "aa_hexdigest": "148b1fbb56239c07daf79d0ce4fc7212", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q50303", "query_cov": 1.0, "subject_cov": 0.8315217391304348, "identity": 1.0, "score": 302.0, "evalue": 2.22e-103, "valid": true, "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_P47412", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47412" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "CNOCLNGIAL_434", "locus": "GCA000143945_00399" }, { "type": "cds", "sequence": "contig_1", "start": 224302, "stop": 224652, "strand": "+", "frame": 1, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625", "RefSeq:WP_010874538.1", "SO:0001217", "UniParc:UPI0000133C7F", "UniRef:UniRef100_Q50302", "UniRef:UniRef50_Q9PQP4", "UniRef:UniRef90_Q50302" ], "nt": "ATGAAAACACGAACAGAACAACGGCGCTTACGCCATAAAAGGATTGTGAAGAAGATCCGCGCCACTAACCACGACAACCGCGTTGTGTTAATGGTGATTAAGTCCTTAAACCATATTAGTGTGCAAGCGTGGGACTTTTCCCAGAACATTGTTTTAGCGTCCTCTTCCTCACTTGCTTTAAAATTAAAGAATGGTAACAAGGACAATGCCAAGTTAGTGGGGCAAGACATTGCTGATAAACTAGTGAAGCTCAAACTAACCAACGTTGTCTTTGACACTGGTGGTAGTAAGTACCACGGCCGGATTGCGGCATTGGCCGAAGCAGCCCGCGAAAGGGGGTTAAACTTCTAA", "aa": "MKTRTEQRRLRHKRIVKKIRATNHDNRVVLMVIKSLNHISVQAWDFSQNIVLASSSSLALKLKNGNKDNAKLVGQDIADKLVKLKLTNVVFDTGGSKYHGRIAALAEAARERGLNF", "aa_hexdigest": "32ec61768f43ea6307058b5153b62ce2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133C7F", "ncbi_nrp_id": "WP_010874538.1", "uniref100_id": "UniRef100_Q50302", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133C7F", "RefSeq:WP_010874538.1", "UniRef:UniRef100_Q50302" ] }, "ips": { "uniref100_id": "UniRef100_Q50302", "uniref90_id": "UniRef90_Q50302" }, "psc": { "uniref90_id": "UniRef90_Q50302", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_Q9PQP4", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP4" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "CNOCLNGIAL_435", "locus": "GCA000143945_00400" }, { "type": "cds", "sequence": "contig_1", "start": 224656, "stop": 225315, "strand": "+", "frame": 1, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "COG:COG0098", "COG:J", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02988", "RefSeq:WP_010874539.1", "SO:0001217", "UniParc:UPI0000135061", "UniRef:UniRef100_Q50301", "UniRef:UniRef50_Q50301", "UniRef:UniRef90_Q50301" ], "nt": "ATGACTGATCAAAACCAAAAAGCAAATCAAGGTAACGGCTTGCAAACCACTAACCTCCAAGCCCACGCACAGCGTAAACACAACCTCCGTCCTTCCTCTGAAGGGATTAAGAAAGCCGTGTCCAAAAAGGAAGGTGGTGGTCACAACCGTAACAACCAAAACCGTCGCTTCCAAAAACCGGCTTTCAAGAGTGAGTTTGAAGAACGCATCGTCAAACTAAAGCGCATTTCTAAAACCACTAAGGGTGGACGGAACATGCGGTTTAGTGTGCTGGTCGTAGTTGGTAACCGCAAGGGCAAGATCGGTTATGGGATTGCTAAGGCCTTGGAAGTACCGAATGCGATTAAGAAAGCCATTAAAGCAGCGCACAACTCACTCCACACAATTGAAATCCACAAGGGTTCAATTTACCACGAAGTAATTGGCCGTAGTGGGGCGAGTCGCGTGTTGCTCAAACCCGCTCCTCAAGGTACGGGAATTATTGCTGGTGGTGCCATTCGGGCCATTATTGAGCTCGCTGGTTACAGTGACATTTACACCAAGAACCTCGGTCGCAACACGCCAATTAACATGATCCATGCCACCATGGATGGGATCTTAAAGCAGCTCTCTCCACGTCGGGTAGCGATCTTACGTAACAAAAACCTCAACGAACTTTAG", "aa": "MTDQNQKANQGNGLQTTNLQAHAQRKHNLRPSSEGIKKAVSKKEGGGHNRNNQNRRFQKPAFKSEFEERIVKLKRISKTTKGGRNMRFSVLVVVGNRKGKIGYGIAKALEVPNAIKKAIKAAHNSLHTIEIHKGSIYHEVIGRSGASRVLLKPAPQGTGIIAGGAIRAIIELAGYSDIYTKNLGRNTPINMIHATMDGILKQLSPRRVAILRNKNLNEL", "aa_hexdigest": "02492a98fc2901ada19459181bae0900", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135061", "ncbi_nrp_id": "WP_010874539.1", "uniref100_id": "UniRef100_Q50301", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135061", "RefSeq:WP_010874539.1", "UniRef:UniRef100_Q50301" ] }, "ips": { "uniref100_id": "UniRef100_Q50301", "uniref90_id": "UniRef90_Q50301" }, "psc": { "uniref90_id": "UniRef90_Q50301", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_Q50301", "cog_id": "COG0098", "cog_category": "J", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q50301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50301" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "CNOCLNGIAL_436", "locus": "GCA000143945_00401" }, { "type": "cds", "sequence": "contig_1", "start": 225319, "stop": 225774, "strand": "+", "frame": 1, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02876", "RefSeq:WP_014325390.1", "SO:0001217", "UniParc:UPI0001DD64E0", "UniRef:UniRef100_A0AAV5N9G4", "UniRef:UniRef50_P47415", "UniRef:UniRef90_Q50300" ], "nt": "ATGGAACTCAATCAACTCAAAAGTGTCCCCAAAGCGCGAAACCACAAGACCAAAACTTTAGGTCGTGGGCACGGCTCGGGTTTAGGTAAAACATCTGGTCGAGGCCAGAAGGGGCAAAAAGCGCGCAAGTCCGGTTTAACCCGTCCTGGTTTTGAAGGAGGACAAACGCCACTGTACCGTCGTTTACCGAAGTTTGGTAACGCACGCAAGGGCTTTTTAAAGCAGGAGTGGGTGGTGTTAAACCTCAATAAAATTGCTAAATTAAAGCTCGACAAAATTAACCGCGCTAGCTTAATTGAAAAGCAAGTAATTAGCGCAAAGAGCCAATTACCAATTAAACTAATTGGCAACACTAAGCTCGAAAAGCCGTTGCACTTTGAGGTGCACAAGGTTTCCAAGCAAGCTTTGAAAGCGGTTGAAAACGCCAACGGTAGTGTGAAGCTGTTAGAGAAATAA", "aa": "MELNQLKSVPKARNHKTKTLGRGHGSGLGKTSGRGQKGQKARKSGLTRPGFEGGQTPLYRRLPKFGNARKGFLKQEWVVLNLNKIAKLKLDKINRASLIEKQVISAKSQLPIKLIGNTKLEKPLHFEVHKVSKQALKAVENANGSVKLLEK", "aa_hexdigest": "de1dc562ff0880811058f25e3bf5aab2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64E0", "ncbi_nrp_id": "WP_014325390.1", "uniref100_id": "UniRef100_A0AAV5N9G4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64E0", "RefSeq:WP_014325390.1", "UniRef:UniRef100_A0AAV5N9G4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9G4", "uniref90_id": "UniRef90_Q50300" }, "psc": { "uniref90_id": "UniRef90_Q50300", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_P47415", "cog_id": "COG0200", "cog_category": "J", "kegg_orthology_id": "K02876", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47415" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "CNOCLNGIAL_437", "locus": "GCA000143945_00402" }, { "type": "cds", "sequence": "contig_1", "start": 225774, "stop": 227207, "strand": "+", "frame": 3, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0005886", "GO:0006605", "GO:0015031", "GO:0016020", "GO:0043952", "GO:0065002", "RefSeq:WP_014325391.1", "SO:0001217", "UniParc:UPI0001DD64E1", "UniRef:UniRef100_A0AAX0SR74", "UniRef:UniRef50_O52351", "UniRef:UniRef90_Q59548" ], "nt": "ATGCAAGCCAAACCAACAACTGCAGTTAAACAAAAACAGAATTTTGGGCAACGTTTGTTCACTTTGTTGCGCAACCGTGACTTCATGATCTCGTTTTTAATAACGGTCGTGCTGTTGGTTTTGTTTCGTGTTTTAGCGATTATTCCGCTCCCCGGCATCCAAGTCAACCAAACCGGGCTAGACCAAAACTCAAACGATTTCTTCTCACTGTTTAACCTACTTGGTGGGGGTGGATTGAACCAACTATCGCTGTTTGCGGTGGGAATTAGTCCGTATATTTCGGCGCAAATTATTATGCAGCTGTTGTCAACGGACTTAATTCCCCCGTTATCTAAACTGGTGAACAGTGGGGAAGTGGGTCGGCGTAAAATCGAGATGATTACGCGGATTATTACGCTACCGTTTGCGTTGGTGCAATCGTTTGCTGTGATCCAAATTGCCACTAATTCCGGTGGCGGTTCTTCACCTATTACCTTAAAAAACAACGGTTCGGATTTTGTCGCGTTTTACATCATTGCCATGACTGCTGGCACCTACCTGTCGGTCTTTTTAGGTGACACCATTTCCAAAAAGGGAATTGGTAACGGGATCACCCTGTTAATTCTTTCGGGGATTTTAGCCCAGCTACCCGAAGGATTTATAGCTGCTTACAGCGTGTTATCAGGTGTAGTGGTAACCATTAACGCAACCTTAACCACTGCCATTAGCTTCTTCATTTACTTCATGGCGTTTTTGGCATTACTGTTTGCCACTACCTTTATTACCCAAGCGACCCGGAAGATTCCGATTCAACAATCCGGTCAGGGTTTAGTAACCGAATCCTCTGCGTTACCTTACCTCCCAATTAAGGTCAACTCCGCCGGGGTAATTCCGGTGATCTTTGCGTCTTCGATTATGTCAATTCCGGTGACGATTGCCCAGTTCCAACCGCAAACCGAATCACGCTGGTTTGTGGAGGACTATCTTTCACTGTCCAAACCAACTGGCATTGTCCTGTATGGTATCTTGGTGATTCTCTTTAGCTTCTTTTACAGCTACATCCAGATCAATCCAGAACGGTTAGCCAAAAACTTCGAGAAGTCCGGGCGTTTTATCCCGGGTATTCGTCCCGGTAAGGATACTGAGAAACACATTGCCAGAGTACTCGTGCGGATTAACTTTATTGGCGCCCCTTTCCTAACGGTGATTGCCATTATTCCTTACATAGTGTCAGCCTTAATCCACTTACCCAACTCCTTAAGTTTAGGGGGTACGGGGATTATTATCATTGTTACCGCTGTGGTTGAGTTTATGTCAGCACTCCGCTCTGCAGCCACCGCTACTAACTACCAACAACTGCGCCGCAACTTGGCGATTGAAGTGCAAAAAACAGCCCAACAAGACAAGGAGGAACAACTGCGCGCTGAAACCCCTGGTATTGGTAACCTCTGGTAA", "aa": "MQAKPTTAVKQKQNFGQRLFTLLRNRDFMISFLITVVLLVLFRVLAIIPLPGIQVNQTGLDQNSNDFFSLFNLLGGGGLNQLSLFAVGISPYISAQIIMQLLSTDLIPPLSKLVNSGEVGRRKIEMITRIITLPFALVQSFAVIQIATNSGGGSSPITLKNNGSDFVAFYIIAMTAGTYLSVFLGDTISKKGIGNGITLLILSGILAQLPEGFIAAYSVLSGVVVTINATLTTAISFFIYFMAFLALLFATTFITQATRKIPIQQSGQGLVTESSALPYLPIKVNSAGVIPVIFASSIMSIPVTIAQFQPQTESRWFVEDYLSLSKPTGIVLYGILVILFSFFYSYIQINPERLAKNFEKSGRFIPGIRPGKDTEKHIARVLVRINFIGAPFLTVIAIIPYIVSALIHLPNSLSLGGTGIIIIVTAVVEFMSALRSAATATNYQQLRRNLAIEVQKTAQQDKEEQLRAETPGIGNLW", "aa_hexdigest": "2b796d7ff43988d8d6d129625471b5aa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64E1", "ncbi_nrp_id": "WP_014325391.1", "uniref100_id": "UniRef100_A0AAX0SR74", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64E1", "RefSeq:WP_014325391.1", "UniRef:UniRef100_A0AAX0SR74" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SR74", "uniref90_id": "UniRef90_Q59548" }, "psc": { "uniref90_id": "UniRef90_Q59548", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_O52351", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0006605", "GO:0015031", "GO:0016020", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_O52351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52351" ], "product": "Protein translocase subunit SecY" }, "genes": [ "secY" ], "id": "CNOCLNGIAL_438", "locus": "GCA000143945_00403" }, { "type": "cds", "sequence": "contig_1", "start": 227207, "stop": 227848, "strand": "+", "frame": 2, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "SO:0001217", "UniParc:UPI0001DD64E2", "UniRef:UniRef100_Q50299", "UniRef:UniRef50_Q50299", "UniRef:UniRef90_Q50299" ], "nt": "ATGGAAAATAAGTTCTTGTTTTTAGGCGCTCCGGGTGTGGGTAAGGGTACACTAGCTAAGCAAGTAGCCAACACCACCGGATTGTTTCACCTTTCCACTGGTGACATCTTTCGCAGTGTGATGCAAGAGCAGGGTGCGTTGAGTCAAACGCTAGCGCACTATATGAACCAGGGTTTGTATGTCCCTGATGAATTGACTAACCAAACCTTTTGGCACTTTGTCACTACCCACCAAAATGAACTCCACAAGGGCTTTATCCTAGATGGGTATCCCCGTACCTTAAACCAACTCGAGTTTTTGCAAAGCAAGTTGCAATTAGACCAGGTGTTTCACCTTAAGCTAAGTGATCCCCAAGTGCTAGTAGCGCGGATCTTAAACCGTTTGGTTTGTCCTAGTTGTGGTAGTGTATACAACAAGCAGAGCAAACCACCATTGAAGGCCAATCAGTGTGATCGTTGCCATGCTACGTTACAAGCGCGCAATGATGACACGGAAGCGGTAATCTTAAAGCGTTTAACGCTTTACGAAGACACCGTTAAACCCTTAATTGAGGCCTTCACCAAGCAGGGCATTCTCACCGTCATTGAAGCCCAATTACCGCTCGAACAACAGGTCAATTTGGTTCAACAGTTAGTGCATTAA", "aa": "MENKFLFLGAPGVGKGTLAKQVANTTGLFHLSTGDIFRSVMQEQGALSQTLAHYMNQGLYVPDELTNQTFWHFVTTHQNELHKGFILDGYPRTLNQLEFLQSKLQLDQVFHLKLSDPQVLVARILNRLVCPSCGSVYNKQSKPPLKANQCDRCHATLQARNDDTEAVILKRLTLYEDTVKPLIEAFTKQGILTVIEAQLPLEQQVNLVQQLVH", "aa_hexdigest": "fd4a177fd8fd988c3ebaacc33fb1ce32", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64E2", "uniref100_id": "UniRef100_Q50299", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64E2", "UniRef:UniRef100_Q50299" ] }, "ips": { "uniref100_id": "UniRef100_Q50299", "uniref90_id": "UniRef90_Q50299" }, "psc": { "uniref90_id": "UniRef90_Q50299", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q50299", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q50299", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50299" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "CNOCLNGIAL_439", "locus": "GCA000143945_00404" }, { "type": "cds", "sequence": "contig_1", "start": 227848, "stop": 228594, "strand": "+", "frame": 1, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006", "KEGG:K01265", "RefSeq:WP_010874543.1", "SO:0001217", "UniParc:UPI0000125A05", "UniRef:UniRef100_Q11132", "UniRef:UniRef50_P47418", "UniRef:UniRef90_Q11132" ], "nt": "ATGGTTTATTTAAAGTCTGCGCGTGAGGTCGAACAGATCCGCCAAGCATGTAAGATTTTCCAAGAAGCAAAGGCATATTTTACAATTGAAAGGCTACTTGGCAAATCTTTAACAGCAATTGATCAAGCGTTAAAGCAGTTTATTGAGAGCAAAGGTGCAACTTGTGCCTTTCATAAATACCAAAATTTCCCGGGATTTAACTGCCTTTCGTTAAACGAAACGGTAATCCATGGCATAGCGGACAACCGTGTTTTTGGTGTTAAGGATAAACTGACGTTGGATATTGGCATTAACCTTAACGGTTATATTTGCGATGCAGCTTTTACGGTTTTGGGCCCCAAGGCTCCAGAGCCCATGCAAACGCTGTTAGAGGTAACGGAAGCTTGTTTTACTGCTGTGGTGGAGCCTCAACTGCGCCCCAATAACCCCACTGGTAATGTTTCCCATGCCATCCAAACCTACTTTGAATCTAAGGGTTATTACCTTTTAAAACAGTTTGGTGGTCATGGTTGTGGGATTAAGGTGCACGAAGAACCGCTGATTTTGAACTATGGTAAGCCTGATACAGGTACTAAGTTAGAACCAGGGATGGTCTTGTGCATTGAGCCCATGGTAATGACTGACAGTGATGCAATGGTAATGCATAACAACAGCTGGAATGTATTAACCCCTAAAAGCCGTTACAATTGTCATGTAGAACAGATGTATGTGATCACCACAAGTGGATTTGAATGTTTAACTAATTAA", "aa": "MVYLKSAREVEQIRQACKIFQEAKAYFTIERLLGKSLTAIDQALKQFIESKGATCAFHKYQNFPGFNCLSLNETVIHGIADNRVFGVKDKLTLDIGINLNGYICDAAFTVLGPKAPEPMQTLLEVTEACFTAVVEPQLRPNNPTGNVSHAIQTYFESKGYYLLKQFGGHGCGIKVHEEPLILNYGKPDTGTKLEPGMVLCIEPMVMTDSDAMVMHNNSWNVLTPKSRYNCHVEQMYVITTSGFECLTN", "aa_hexdigest": "2f1b193bf84ffb3adf81d5e2d92c6692", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125A05", "ncbi_nrp_id": "WP_010874543.1", "uniref100_id": "UniRef100_Q11132", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125A05", "RefSeq:WP_010874543.1", "UniRef:UniRef100_Q11132" ] }, "ips": { "uniref100_id": "UniRef100_Q11132", "uniref90_id": "UniRef90_Q11132" }, "psc": { "uniref90_id": "UniRef90_Q11132", "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_P47418", "cog_id": "COG0024", "cog_category": "J", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_P47418", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47418" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "CNOCLNGIAL_440", "locus": "GCA000143945_00405" }, { "type": "cds", "sequence": "contig_1", "start": 228594, "stop": 228830, "strand": "+", "frame": 3, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022", "KEGG:K02518", "RefSeq:WP_010874544.1", "SO:0001217", "UniParc:UPI000012D263", "UniRef:UniRef100_Q50298", "UniRef:UniRef50_Q50298", "UniRef:UniRef90_Q50298" ], "nt": "ATGCAACCAAAGTTTAATAACCAAGCAAAGCAGGACAAACTCGTCTTAACGGGCAAAATCTTGGAAATCATCCACGGTGACAAGTTTCGCGTGTTGTTGGAAAACAACGTTGAAGTAGATGCTCACTTAGCGGGCAAAATGCGAATGCGCCGGTTACGAATTTTACCGGGCGATTTAGTTGAAGTGGAATTTTCCCCTTATGATTTAAAGTTAGGTCGAATAATAGGTAGAAAATAA", "aa": "MQPKFNNQAKQDKLVLTGKILEIIHGDKFRVLLENNVEVDAHLAGKMRMRRLRILPGDLVEVEFSPYDLKLGRIIGRK", "aa_hexdigest": "95747ef8ca2e0f64bb414f5618436f45", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012D263", "ncbi_nrp_id": "WP_010874544.1", "uniref100_id": "UniRef100_Q50298", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D263", "RefSeq:WP_010874544.1", "UniRef:UniRef100_Q50298" ] }, "ips": { "uniref100_id": "UniRef100_Q50298", "uniref90_id": "UniRef90_Q50298" }, "psc": { "uniref90_id": "UniRef90_Q50298", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q50298", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q50298", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50298" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "CNOCLNGIAL_441", "locus": "GCA000143945_00406" }, { "type": "cds", "sequence": "contig_1", "start": 228954, "stop": 229211, "strand": "+", "frame": 3, "gene": null, "product": "30S ribosomal protein S13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "nt": "ATGGCACGGATTTTAGGTATTGACATTCCCAACCAGAAACGCATAGAAATTGCGTTAACCTATATTTTTGGTATAGGGCTTTCGCGCTCCCAAGCCATCTTAAAGCAAGCGAACATTAACCCCGATAAACGGGTCAAAGACTTAACCGAAGAGGAGTTTGTTGCCATTAGGAATGTAGCTAGTGCTTACAAGATTGAGGGTGATTTGCGTCGTGAAATTGCACTCAACATTAAGCATTTGAGTGAAATTGGAGCATGA", "aa": "MARILGIDIPNQKRIEIALTYIFGIGLSRSQAILKQANINPDKRVKDLTEEEFVAIRNVASAYKIEGDLRREIALNIKHLSEIGA", "aa_hexdigest": "7d2df25be7fa43522d3bb68be647b043", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031B5D25D", "query_cov": 1.0, "subject_cov": 0.9770114942528736, "identity": 0.565, "score": 95.5, "evalue": 4.27e-24, "valid": false, "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_A0A535XWH1", "kegg_orthology_id": "K02952" }, "pscc": { "uniref50_id": "UniRef50_A0A535XWH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "product": "30S ribosomal protein S13" }, "genes": [], "id": "CNOCLNGIAL_442", "locus": "GCA000143945_00407" }, { "type": "cds", "sequence": "contig_1", "start": 229328, "stop": 229438, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAGAAGAAAAAGATTAATGTATCTAGTGGAATTATCCACGTATCCTGTTCCCCAAATAACACAATTGTATCCGCTAGTGATCCGGGGGGCAACGTGTTGTGCTGA", "aa": "MAKKKKINVSSGIIHVSCSPNNTIVSASDPGGNVLC", "aa_hexdigest": "6fa3ed66a08dcd96c26be2d606006744", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3670.243200000001, "isoelectric_point": 9.303384208679198 }, "id": "CNOCLNGIAL_443", "locus": "GCA000143945_00408" }, { "type": "cds", "sequence": "contig_1", "start": 229454, "stop": 229693, "strand": "+", "frame": 2, "gene": "rpsK", "product": "30S ribosomal protein S11", "db_xrefs": [ "COG:COG0100", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02948", "SO:0001217", "UniRef:UniRef50_Q50296", "UniRef:UniRef90_Q50296" ], "nt": "ATGGGCTTTAAGGGATCGCGCAAAAAGACCCCGTACTCAGCCGGCATAGCAGCCGATAAGGTGGCCAAAACCGTCAAGGAAATGGGGATGGCGACCGTTAAGCTCTTTGTCAAGGGTACAGGTCGGGGTAAGGATACCGCCATTCGCAGCTTTGCTAATGCGGGACTTTCTATTACTGAAATTAACGAAAAGACCCCGATTCCCCACAACGGTTGCAAACCACCAAAACGACCACGCTAA", "aa": "MGFKGSRKKTPYSAGIAADKVAKTVKEMGMATVKLFVKGTGRGKDTAIRSFANAGLSITEINEKTPIPHNGCKPPKRPR", "aa_hexdigest": "7f2dd1aa2c4078964274d355c8fc4a0b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A7Z0ENF1", "query_cov": 1.0, "subject_cov": 0.8061224489795918, "identity": 0.544, "score": 94.7, "evalue": 9.23e-24, "valid": false, "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_A0A2H5B013", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "genes": [ "rpsK" ], "truncated": "5-prime", "pseudogene": { "start": 229328, "stop": 229693, "observations": { "mutation": [ 229436 ], "stop": [ 229436 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50296", "query_cov": 1.0, "subject_cov": 0.6528925619834711, "identity": 1.0, "score": 161.0, "evalue": 8.04e-50, "gene_start": 1, "gene_end": 79, "reference_start": 43, "reference_end": 121, "reference_sequence": "MAKKKKINVSSGIIHVSCSPNNTIVSASDPGGNVLCWASSGTMGFKGSRKKTPYSAGIAADKVAKTVKEMGMATVKLFVKGTGRGKDTAIRSFANAGLSITEINEKTPIPHNGCKPPKRPR" }, "paralog": false, "identity": 0.9917355371900827, "score": 241.0, "evalue": 9.26e-84, "description": "Internal stop codon at 229436. Nonsense mutation around 229436.", "psc": { "uniref90_id": "UniRef90_Q50296", "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_Q50296", "cog_id": "COG0100", "cog_category": "J", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50296", "UniRef:UniRef50_Q50296", "COG:COG0100", "COG:J", "KEGG:K02948", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q50296", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50296" ], "product": "Small ribosomal subunit protein uS11" } }, "id": "CNOCLNGIAL_444", "locus": "GCA000143945_00409" }, { "type": "cds", "sequence": "contig_1", "start": 229699, "stop": 230025, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01193.29" ], "nt": "ATGGAAAAGTTTTTAAAGTACGAAATAAAGGTAAATAACGAACAAGCACGCGCTAACCCCAACTACGGCATCTTTGAAGTTGGACCACTCGAATCGGGCTTTGGCATTACCATCGGTAATGCTATGCGCCGGGTGCTGTTGAGCTGTATTCCAGGTGCCAGTGTGTTTGCACTCTCAATTTCGGGTGCCAAGCAAGAGTTTGCTGCTGTGGAAGGGATGAAGGAAGATGTCACTGAAGTGGTGTTAAACTTCAAGCAATTGGTAGTGAAGATTTCTGACCTTCTGTTTGAAGATGGTGAAATGGTCGAACCACCACTAGAACGTTGA", "aa": "MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFGITIGNAMRRVLLSCIPGASVFALSISGAKQEFAAVEGMKEDVTEVVLNFKQLVVKISDLLFEDGEMVEPPLER", "aa_hexdigest": "a567bbe0c202bfbdc5329a463bb79ed1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01193.29", "name": "RNA_pol_L", "length": 64, "aa_cov": 0.5740740740740741, "hmm_cov": 0.765625, "evalue": 1.717564922260112e-10, "score": 35.988704681396484, "start": 29, "stop": 90 } ], "seq_stats": { "molecular_weight": 11923.654199999995, "isoelectric_point": 4.7362455368042 }, "id": "CNOCLNGIAL_445", "locus": "GCA000143945_00410" }, { "type": "cds", "sequence": "contig_1", "start": 230029, "stop": 230682, "strand": "+", "frame": 1, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P47423", "UniRef:UniRef90_Q50295" ], "nt": "TTGTTAACGGTAACAGCTGAGAAAGCGGGACCGGTGTATGCGAAGGACTTGGAGTGCCCAGCTGGCTTTGAAGTGGTCAACAAGGACTTGTACCTGTTCTCGTTGCAAACTGACAAAAAGGTAACAGTTAACGTGTATGTGAAACAAGGACGGGGGTTTGTTACTTTCTTGGAAAACCGTGAAATGATTAATTCTTTGGGGATTATTGCCACTGACTCGAACTTCTCCCCGGTATTACACTGTGGTTATGAAGTACAGGAATTGAAAACTTCCAAGCAAAAGATTACTGATCACTTAACTTTCAAAATTGCTACTAACGGTGCCATATCCGCTGTGGATGCCTTTGCAATGGCTGCAAAGATCTTGATCGAACACCTCAATCCGATTGTTAATGTCAACGAATCGATTAAGGCGTTGAATATTATCCAAGAGAAAGCCGAAGAACGCCGTGTCCGTTCGTTTGCGAAGCAAATTGAAGAGTTGGACTTTACCGTGCGTACCTTTAACTGTTTGAAGCGTAGTGGGATCCACACCTTACAAGAGTTGTTGTCCAAGTCATTAGCTGATATTCGTGAAATCCGTAACCTCGGTAAGAAATCGGAACGCGAAATTATTAAGAAGGTACACGAATTAGGACTCAAATTAAGATCTTAG", "aa": "MLTVTAEKAGPVYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQGRGFVTFLENREMINSLGIIATDSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIVNVNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIREIRNLGKKSEREIIKKVHELGLKLRS", "aa_hexdigest": "cbf888f5bf323cea9f11ef7210ca5bfd", "start_type": "TTG", "rbs_motif": null, "genes": [ "rpoA" ], "truncated": "5-prime", "pseudogene": { "start": 229729, "stop": 230682, "observations": { "mutation": [ 230023 ], "stop": [ 230023 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50295", "query_cov": 1.0, "subject_cov": 0.6636085626911316, "identity": 0.995, "score": 414.0, "evalue": 1.49e-144, "gene_start": 1, "gene_end": 217, "reference_start": 111, "reference_end": 327, "reference_sequence": "MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFVITIGNAMRRVLLSCIPGASVFALSISGAKQEFAAVEGMKEDVTEVVLNFKQLVVKISDLLFEDGEMVEPPLERWPLLTVTAEKAGPVYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQGRGFVTFLENREMINSLGIIATDSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIVNVNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIREIRNLGKKSEREIIKKVHELGLKLRS" }, "paralog": false, "identity": 0.9936908517350158, "score": 607.0, "evalue": 1.09e-222, "description": "Internal stop codon at 230023. Nonsense mutation around 230023.", "psc": { "uniref90_id": "UniRef90_Q50295", "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47423", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50295", "EC:2.7.7.6", "UniRef:UniRef50_P47423", "COG:COG0202", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P47423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47423" ], "product": "DNA-directed RNA polymerase subunit alpha" } }, "id": "CNOCLNGIAL_446", "locus": "GCA000143945_00411" }, { "type": "cds", "sequence": "contig_1", "start": 230730, "stop": 231059, "strand": "+", "frame": 3, "gene": "rplQ", "product": "50S ribosomal protein L17", "db_xrefs": [ "COG:COG0203", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02879", "SO:0001217", "UniRef:UniRef50_P47424", "UniRef:UniRef90_Q59547" ], "nt": "ATGACCGTGCGCCAACAGGTAAGCGCTGTTTTGGCTTATGGCAAGATTGAAACAACCTTAAAGAAGGCCAAGAACACCCAAAAGCGTTTAGATAAGTTAATTACCTTAGCTAAAGTTGATAACTTTAATAACCGTCGTCAAGTCAAAAAGTGGTTGTTAAACACCAACTTGTTCGATGTTGATCAACTAATGGATCACTTGTTTAGCAAAGTTGCCCCTAAATACGAAAAAACCCCCGGTGGTTACAGCCGTGTTTTAAAATTAGGGCCACGTCGCGGAGATGCCACGGAAATGGCCATCTTGCAACTAACTGATGCTAAATACAAATAA", "aa": "MTVRQQVSAVLAYGKIETTLKKAKNTQKRLDKLITLAKVDNFNNRRQVKKWLLNTNLFDVDQLMDHLFSKVAPKYEKTPGGYSRVLKLGPRRGDATEMAILQLTDAKYK", "aa_hexdigest": "58f9dbd146e31873ccf00c49f2709be5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q59547", "query_cov": 1.0, "subject_cov": 0.8790322580645161, "identity": 1.0, "score": 215.0, "evalue": 1.38e-70, "valid": true, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P47424", "cog_id": "COG0203", "cog_category": "J", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47424" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [ "rplQ" ], "id": "CNOCLNGIAL_447", "locus": "GCA000143945_00412" }, { "type": "cds", "sequence": "contig_1", "start": 231043, "stop": 231858, "strand": "+", "frame": 1, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:7.-.-.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47425", "UniRef:UniRef90_Q50294" ], "nt": "ATGCTAAATACAAATAAACAAGCAGCTGCAAGCTTTCAAGGTGTTTATTTTTCTTACAGCGAATTACCATTAATTCGCAACCTCTCCTTTGATGTTTTCGAAGGGGAGTATGTTTGCATAGTTGGCCATAACGGCTCGGGTAAATCCACCATCTCGAAGCTGTTAACGGGCTTGTTAAAACCACAAGCGGGTACCATCAAAATCTTTGGTAAGACGATTTCGAGTGAAAACGTGACTTACCTACGCAACCACATTGGCATTATCTTCCAAAACCCCGATAACCAGTTTATTGGTATTACCGTGGAAGATGACATTGCTTTTGGACTGGAAAACCGCCGTTATACGCGCGAAAAGATGAAGAGCATCATTGAGGATGTAGCCCAACAAGCGGGAATAACGGAGTTATTGCAAAAAGAACCGCATAACCTTTCGGGTGGGCAAAAGCAACGCGTTGCCATTGCCTCGGTGTTAGCCTTAAACCCAGCCATCATTATCTTTGATGAGTCGACTTCGATGTTGGATCCTAAAGCGAAGCGTACCATTAAGCAGTTCATGGTGGAACTGCGCAACCAGGGCAAGTGTGTTATCTCCATTACCCATGACATGGAAGAGGTTACCAAAGCAGACAAGGTTTTGGTCATGAATGAAGGTCGTTTAATTCGTCAAGGTAAACCCAAGGATGTCTTTAACAGTGCAGCCGAATTGCAAAAAATCCGCTTGGATATTCCCTTTTCTTTAAGCCTTTCCACGAAGGTTAATGGTGTGACTAGTACAATAGACTACCAACAACTAATTGAGGCCATTGGTAAGCTATGA", "aa": "MLNTNKQAAASFQGVYFSYSELPLIRNLSFDVFEGEYVCIVGHNGSGKSTISKLLTGLLKPQAGTIKIFGKTISSENVTYLRNHIGIIFQNPDNQFIGITVEDDIAFGLENRRYTREKMKSIIEDVAQQAGITELLQKEPHNLSGGQKQRVAIASVLALNPAIIIFDESTSMLDPKAKRTIKQFMVELRNQGKCVISITHDMEEVTKADKVLVMNEGRLIRQGKPKDVFNSAAELQKIRLDIPFSLSLSTKVNGVTSTIDYQQLIEAIGKL", "aa_hexdigest": "3c727d3ba5186aa4c199538b80687fe0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q50294", "query_cov": 1.0, "subject_cov": 0.9890510948905109, "identity": 1.0, "score": 525.0, "evalue": 5.27e-188, "valid": true, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "7.-.-.-" ], "uniref50_id": "UniRef50_P47425", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_P47425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47425" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA1" ], "id": "CNOCLNGIAL_448", "locus": "GCA000143945_00413" }, { "type": "cds", "sequence": "contig_1", "start": 231855, "stop": 232766, "strand": "+", "frame": 3, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:3.6.3.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_010874551.1", "SO:0001217", "UniParc:UPI00001393F6", "UniRef:UniRef100_Q50293", "UniRef:UniRef50_Q50293", "UniRef:UniRef90_Q50293" ], "nt": "ATGAAAAAAATAGTTGAACTCAAAAACCCCTTAAGGGATGGAGAAATCCTCAGTGTTAGTAACCTTTCGTGCTTTTTTAACGAAAAAACAGTCCATGAAGTCAAGGTAATTGACAACTTTAGCTATACCTTTGCTAAAAATAAGGTCTACTGCATTATTGGTGACAGTGGTAGTGGTAAATCAACCTTAGTCAACCATTTTAATGGTTTAATAAAGCCCGCTTACGGAGATATTTGGGTCAAGGATATTTACATTGGTCAAAAGCAACGCAAAATTAAGGACTTTAAACGCTTGCGCAAAACAATTTCGATTGTCTTTCAGTTTCCCGAATACCAACTGTTTAAGGACACAGTGGAAAAGGACATTATGTTCGGTCCAATTGCTTTGGGTCAATCGAAAAACGAAGCACGCCAAAAGGCAGCTTACTACCTCGAAAAAATGGGGTTAAAGTACACTTTTTTAGAGCGCAATCCGTTTGAGTTGAGCGGGGGGCAAAAGCGCCGTGTAGCGATTGCTGGGATTTTAGCAATTGAACCCGAAGTTTTAATCTTTGATGAACCTACCGCTGGACTAGATCCTGAAGGGGAACGAGAAATGATGCGTTTAATTAAGGATGCTAAAGCTAGTGGTCGCACCGTCTTTATGATTACCCACCAAATGGAAAATGTTTTGGAAGTAGCGGATGAAGTGCTGGTGTTGTCCAAGGGCCAACTCGTTAAATCCGGTGATCCTTACGAAGTTTTTATGGATGAAGCATTTTTGGCACACACCACCATTATTATGCCTCCAGTCATTCAAGTTATCAAAGACCTAATTAACTTAAACCCGAAGTTTAGTTGTTTATTAGACTTTAAACCGCGCAACTTAGACCAGTTGGCGGATGCCATTAACAACACAATAGCTCATGGATAG", "aa": "MKKIVELKNPLRDGEILSVSNLSCFFNEKTVHEVKVIDNFSYTFAKNKVYCIIGDSGSGKSTLVNHFNGLIKPAYGDIWVKDIYIGQKQRKIKDFKRLRKTISIVFQFPEYQLFKDTVEKDIMFGPIALGQSKNEARQKAAYYLEKMGLKYTFLERNPFELSGGQKRRVAIAGILAIEPEVLIFDEPTAGLDPEGEREMMRLIKDAKASGRTVFMITHQMENVLEVADEVLVLSKGQLVKSGDPYEVFMDEAFLAHTTIIMPPVIQVIKDLINLNPKFSCLLDFKPRNLDQLADAINNTIAHG", "aa_hexdigest": "6d515d943aee2ee12155c598a96e60ca", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001393F6", "ncbi_nrp_id": "WP_010874551.1", "uniref100_id": "UniRef100_Q50293", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393F6", "RefSeq:WP_010874551.1", "UniRef:UniRef100_Q50293" ] }, "ips": { "uniref100_id": "UniRef100_Q50293", "uniref90_id": "UniRef90_Q50293" }, "psc": { "uniref90_id": "UniRef90_Q50293", "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "3.6.3.-" ], "uniref50_id": "UniRef50_Q50293", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_Q50293", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50293" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" }, "genes": [ "ecfA2" ], "id": "CNOCLNGIAL_449", "locus": "GCA000143945_00414" }, { "type": "cds", "sequence": "contig_1", "start": 232759, "stop": 233100, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGTTTTTTTATTAATGGTTATGTACCACGAGATACCTTTATCCACCGGCTCCACCCGACAACGAAACTGTTAATCTTTTTGCTGTTCGTGATCCTGGTGTTTGTACCCATTGGCTTTGTTTTTCAAAGTGTGATCTTTGTCTTTGCCACAGTTATTTTCTTTGTAGCCAAACTACCCGGACGGTTTTACCTCTCCTCGATTAAGTCAATCTCACTGTTATTCTTACTACTGTTATTTGTCAACTGGTTTACCTTCCGTGACCCCGGGTTCTACATTACCGCTGATCAGGTTAATACAGTAAAACCACACTTTAACGGTAATAACTTTAACTTCTGA", "aa": "MDSFFINGYVPRDTFIHRLHPTTKLLIFLLFVILVFVPIGFVFQSVIFVFATVIFFVAKLPGRFYLSSIKSISLLFLLLLFVNWFTFRDPGFYITADQVNTVKPHFNGNNFNF", "aa_hexdigest": "db2dfa0461e906685a91c52d88b2aa7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13196.49359999998, "isoelectric_point": 9.868062782287598 }, "id": "CNOCLNGIAL_450", "locus": "GCA000143945_00415" }, { "type": "cds", "sequence": "contig_1", "start": 233131, "stop": 233604, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTCGCAAGTGTTTAACTTTAACCGTGCCAACATGACAGAGTTAAACAAGATTAACTTCTTCTTTAAGGAAACAGCTAATGCTAATGCATACACCAAGGTAACGGGGATTGATAAATTGGCAGAAATGTTGGCTTCAAAGAATCTCTTTAAGTTCAACGGTTCTACCAATGGCATTGATAAAAACAAGATTCTTGGTGCCTTTCTAGATCATAAGATTGCCGTTTACCTCGGTCGTAGTTGGGGTGGTGACTTTAGTGGTTTTGTGATTGACGTAAGTGTAAGTGATAAAACATCTACCTTCACGATTAAACCCTTTTTAGCTAACTCGAACTATGTGTTGACACTGCGTGCCATTATTCTAGCCTTTTACGTTACCCAAAAGATCTTGATCATGATCATTTTGGCAACAGTGTTAACTTCCACTTCAAGCTCGGTTGAGTTGGCATACGGAATTGAACGCTTGCTGTGA", "aa": "MFSQVFNFNRANMTELNKINFFFKETANANAYTKVTGIDKLAEMLASKNLFKFNGSTNGIDKNKILGAFLDHKIAVYLGRSWGGDFSGFVIDVSVSDKTSTFTIKPFLANSNYVLTLRAIILAFYVTQKILIMIILATVLTSTSSSVELAYGIERLL", "aa_hexdigest": "15aba9bee48ef64822d4ee107cc53f6f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17461.085499999997, "isoelectric_point": 9.486925697326662 }, "id": "CNOCLNGIAL_451", "locus": "GCA000143945_00416" }, { "type": "cds", "sequence": "contig_1", "start": 233608, "stop": 234063, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGCTGTTACGGGTTCCAGTCAATGTGTTTGCGATGACAATTGCAATTGCAATTCGCTTTGTCCCTTCTTTATTGTTGGAATCACAACGGATTTTAAATGCCCAAGCATCACGCGGTTTGGACTTTAAGAACGGCAATTTCTTTGTCAAAATGCGTTCATTGTCTTCCTTGGTTGTACCGATGATTTCGATTGCATTCCGCAATGCTGGGGAGTTGGCGAGTGCCATGGAAGCACGTGGTTATGATCCAACTAAGAAACGTACTACCTACCGCAAGTTTAAGATTGATTGGGTTGATGCCACAGCTTTAATTCTCACCGCTCTTTACTTTGTGGTGATTATTTTCTTAACCGTTAAGGGTGCAGTGTTCCTAGATTTAGGGACACCTGAGTGGTTGCTAACCGGCAAGATTAAAGAGCAGGTAGAGCGCTCATTATCAGTTAAAAGTGCCTAG", "aa": "MKLLRVPVNVFAMTIAIAIRFVPSLLLESQRILNAQASRGLDFKNGNFFVKMRSLSSLVVPMISIAFRNAGELASAMEARGYDPTKKRTTYRKFKIDWVDATALILTALYFVVIIFLTVKGAVFLDLGTPEWLLTGKIKEQVERSLSVKSA", "aa_hexdigest": "092427315b7d96369286f153f0d841c0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16885.89759999999, "isoelectric_point": 10.232824897766111 }, "id": "CNOCLNGIAL_452", "locus": "GCA000143945_00417" }, { "type": "cds", "sequence": "contig_1", "start": 234218, "stop": 234439, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATGCCATTAACCAAACCTTTCACGTTGATCTACCTGATAAGTTACCACTCAAACAGTTAATTGCCAAACTAAACCAATTAAACTACCCTGACATTTTTGTAAAGGCCTTAGTTCCAGTAGCACCGCGATTTCACGCGCGGTTTGCAGTAAAAACTAAGGTTTATGAATACCTTATTAACACCCGAGCTTTTAACCCAGCACAGTACCACACCGTGTGA", "aa": "MHAINQTFHVDLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTV", "aa_hexdigest": "0b4ed275fcc0223680c33b317db5effd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8488.865399999997, "isoelectric_point": 9.820742988586424 }, "id": "CNOCLNGIAL_453", "locus": "GCA000143945_00418" }, { "type": "cds", "sequence": "contig_1", "start": 234455, "stop": 234787, "strand": "+", "frame": 2, "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "db_xrefs": [ "COG:COG0101", "COG:J", "EC:5.4.99.12", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147", "SO:0001217", "UniRef:UniRef50_P47428", "UniRef:UniRef90_Q50291" ], "nt": "TTGGATTTAGCACAACTTGAAGCGGATACCACGCTCTTTTTAGGCAGGAAGGATTTTTGGAGTTTTAGCACCGCTAGCCAAAAGGACACGCAGCGCACAATTAGTAAAATTATCGTGCAACAAGATCACACTGGTACGGTTAAACTGACCTTTTTTGGCAACGGTTTTTTAAGAAACCAGATTCGAATGATGGTAGCTTCTCTAGTGGCCTTAAACAATCGAACCCTGATGCGAGAAACAGTTCAACAGCTGTTTGATCAACCACAAAAGGGTGGGTGTAAGGTTAAAGCCCCCGCTTGTGGTCTTTATTTAAAAACTGTCTTATATGAATAA", "aa": "MDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVLYE", "aa_hexdigest": "f5672fd11b674d80eb5d9d5e690c2004", "start_type": "TTG", "rbs_motif": null, "genes": [ "truA" ], "truncated": "5-prime", "pseudogene": { "start": 234155, "stop": 234787, "observations": { "mutation": [ 234437 ], "stop": [ 234437 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50291", "query_cov": 1.0, "subject_cov": 0.45267489711934156, "identity": 0.991, "score": 220.0, "evalue": 7.31e-71, "gene_start": 1, "gene_end": 110, "reference_start": 134, "reference_end": 243, "reference_sequence": "MPRYLAIVAYNGAPFRGWAKQPGYHTVQGQIEQNLALIYQTPINIYGSGRTDKGVHAINQTFHVDLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTVWQYNHPLDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVLYE" }, "paralog": false, "identity": 0.9952380952380953, "score": 421.0, "evalue": 1.37e-152, "description": "Internal stop codon at 234437. Nonsense mutation around 234437.", "psc": { "uniref90_id": "UniRef90_Q50291", "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "ec_ids": [ "5.4.99.12" ], "uniref50_id": "UniRef50_P47428", "cog_id": "COG0101", "cog_category": "J", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50291", "EC:5.4.99.12", "UniRef:UniRef50_P47428", "COG:COG0101", "COG:J", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147" ] }, "pscc": { "uniref50_id": "UniRef50_P47428", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47428" ], "product": "tRNA pseudouridine synthase A" } }, "id": "CNOCLNGIAL_454", "locus": "GCA000143945_00419" }, { "type": "cds", "sequence": "contig_1", "start": 234984, "stop": 235181, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTAACAAGCACGCTGAAAACAGTTTGGACAAAACGATTAACGACGCTTTATTTCAGTACGAAATGTTTAAAAGTGAACATGCCTTGGTGTTTGTTGACTTTGAAAAGAACCTGTTTAAGCACGAAAAGGTGATTCGCGCATACCTCCAAGACCCTGCTTTAAAACAATACCAAAGGGATTTTGAACTAGTATGA", "aa": "MSNKHAENSLDKTINDALFQYEMFKSEHALVFVDFEKNLFKHEKVIRAYLQDPALKQYQRDFELV", "aa_hexdigest": "e91f966ec6cab7e0ec89fe83b197b7ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7822.796200000001, "isoelectric_point": 5.83767147064209 }, "id": "CNOCLNGIAL_455", "locus": "GCA000143945_00420" }, { "type": "cds", "sequence": "contig_1", "start": 235221, "stop": 235424, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGCGCAAATTAGTCCGGCATTAAACCAAGCGGACAAGATCTTTAATGTGTTATCTACCGCTGACCTAAACCTCCAACCAGTCGTATACAAGGGTAAAACTTACGTTATTAACGCGGTTAGTGATTATCAAAGTCTCTTGGAAAATAAGGATCGTGGCCTGCGTGAAGCTGCTTACAAAGTGTGGTTGGAAATTTACTGA", "aa": "MLAQISPALNQADKIFNVLSTADLNLQPVVYKGKTYVINAVSDYQSLLENKDRGLREAAYKVWLEIY", "aa_hexdigest": "96c7581ad1c48d323b02b29c4bc4a772", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7604.645400000003, "isoelectric_point": 6.080260276794433 }, "id": "CNOCLNGIAL_456", "locus": "GCA000143945_00421" }, { "type": "cds", "sequence": "contig_1", "start": 235446, "stop": 235802, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTTTAGTGGAAAACTACATCCAATTAGAAACCTTTGCTAAGTTAAAGAAACACCCCAATTACATTGCCAAAACAGCTTTTGATGATGAAATTGATGTGGCTTTCATTGACTTTGTTTATGAGCAAGTGGCTAGCTTTGCCCCGACCTTTAAAGCCTTTCAAAGTTTAAGGAAGCAAATTTACAAGCACGTTTTAAAACTAGACAAAGCCCAACCTTGGGACTTGTCGGTACCGTTGTTTAAGGCTAGTGGTGACTACACCATTGAACAAGCCCAAACTGATGCGCTTAAGATATTAGCACCTATGGGTAGTGAATACCTGGAAGTGGTAAAGGAAGCCTTTCGGGAGCGCTGA", "aa": "MSLVENYIQLETFAKLKKHPNYIAKTAFDDEIDVAFIDFVYEQVASFAPTFKAFQSLRKQIYKHVLKLDKAQPWDLSVPLFKASGDYTIEQAQTDALKILAPMGSEYLEVVKEAFRER", "aa_hexdigest": "32012e5235e7afde35b71028d3387805", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13640.518099999988, "isoelectric_point": 5.671246910095214 }, "id": "CNOCLNGIAL_457", "locus": "GCA000143945_00422" }, { "type": "cds", "sequence": "contig_1", "start": 235887, "stop": 236534, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTGATAAAACCCGCGCTTCATTAAATACCCTAGTCCATGAATTGGGTCACAGTGTCCATTCTTGGTATGCATCTAAGTACCAAACGCAAAACTTGGATCCTACAATTTTCTATGCTGAAATTGCTTCCATTTGTAACGAATTACTGTTGTGTTACCACGATATTATTAACTATGAAAACCGCAATCCGCAGCAGTTAATCCGTAGTTTAATGGAACAAATTAGCCACTTCTTTGGCGCTACCACGAGACAGTTAATGTTCTCTCAGTTTGAACAAGACACCTTAAAGTTAATTCAGCAAAACCAAAAGCCTGACTTTAAGACCCTAGTGGAAATTTATGGTAAGACCGCCATTAAGTACCAAGCTGCTAATGCTGACGCTATTACGAAAAAGCTCAAGCAAACCAAGTACCAAAAGTCATTGGCTTACATTACCTCCATTCCGCATTTTTACGCTGGTAACTTCTATGTTTACAAGTACGCCATTGGTCAAGTTGTGGGTACCTTAGTTGGTAAAAAACTGAGTGCTGGTGACAGTAATATGCTCGCTGCTTACAAGCGCTTCTTGTCTTCAGGCTCCACCTTACCACCACTAGAAACAATTAAATTGTTAGGGATTGACCTTACCCAACCAGAACCGTGA", "aa": "MNFDKTRASLNTLVHELGHSVHSWYASKYQTQNLDPTIFYAEIASICNELLLCYHDIINYENRNPQQLIRSLMEQISHFFGATTRQLMFSQFEQDTLKLIQQNQKPDFKTLVEIYGKTAIKYQAANADAITKKLKQTKYQKSLAYITSIPHFYAGNFYVYKYAIGQVVGTLVGKKLSAGDSNMLAAYKRFLSSGSTLPPLETIKLLGIDLTQPEP", "aa_hexdigest": "9b815534645efd0e635c0cdcc39bc895", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24490.7961, "isoelectric_point": 9.026621437072755 }, "id": "CNOCLNGIAL_458", "locus": "GCA000143945_00423" }, { "type": "cds", "sequence": "contig_1", "start": 236615, "stop": 237094, "strand": "+", "frame": 2, "gene": "mte1", "product": "Type II methyltransferase M-MpnI", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0032259", "SO:0001217", "UniRef:UniRef50_Q50290", "UniRef:UniRef90_Q50290" ], "nt": "GTGCATTACTTTAACCGCGCCAAAAAGGCAAAGAACAATGAATTTTATACCTTGTTTGAAGACATTGCCGCTGAAGTGGCATGTTATCCCAATGCCTTTAAGGGTAAGGTAGTGTTGTGTAATTGTAATGATGGTTACCAATCGAACTTCTGGCAGTTTTTTCAAAGTCAGTTTCATGCTTTAGGACTCAAAAAACTAGTTGCGATTGCTTTTAATCCACTTGGCAACAGTTATCAACTCAACTTCGATGGAAAGGAAATAAAGGAACTACCTTTAGCTGGTAATGGCAGCTTTGACAGTGCGGAAGCCATTGTTCTTTTAAAGCAAAGTGACATTGTGGTAACTAATCCACCGTTTAGCTTGTTTCAGGACTTTGTTTGTCTTTTAGCAGAGCATGGCAAACAGTTTTTGGTTTTAGGTCACAATGGTGCTGTCGGTTATAATCAGATCTTTAAGCTGTTTAAGGAAGAACAGTTGTGA", "aa": "MHYFNRAKKAKNNEFYTLFEDIAAEVACYPNAFKGKVVLCNCNDGYQSNFWQFFQSQFHALGLKKLVAIAFNPLGNSYQLNFDGKEIKELPLAGNGSFDSAEAIVLLKQSDIVVTNPPFSLFQDFVCLLAEHGKQFLVLGHNGAVGYNQIFKLFKEEQL", "aa_hexdigest": "53cfc47731aef104e2fba8fda9c411f3", "start_type": "GTG", "rbs_motif": null, "genes": [ "mte1" ], "truncated": "3-prime", "pseudogene": { "start": 236615, "stop": 237397, "observations": { "mutation": [ 237092, 237257, 237362 ], "stop": [ 237092, 237257, 237362 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50290", "query_cov": 1.0, "subject_cov": 0.49843260188087773, "identity": 1.0, "score": 330.0, "evalue": 1.56e-112, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MHYFNRAKKAKNNEFYTLFEDIAAEVACYPNAFKGKVVLCNCNDGYQSNFWQFFQSQFHALGLKKLVAIAFNPLGNSYQLNFDGKEIKELPLAGNGSFDSAEAIVLLKQSDIVVTNPPFSLFQDFVCLLAEHGKQFLVLGHNGAVGYNQIFKLFKEEQLWYGHTVNSSMLFQVQSNFKLYDPKSVNFVKKDGQLFQKVPGISWFTNLKKNQQPAWLKTKSRYQGNEHKYPKFDWYDAIFVSKVKEIPLDWFGYMGVPLTFLNCFNPKQFELIDCLANPYATLDTLKTNAYVRSHHGDVRNVKGKRRYVRVVIKQRQNVI" }, "paralog": false, "identity": 0.9807692307692307, "score": 524.0, "evalue": 3.24e-191, "description": "Internal stop codon at 237092, 237257, 237362. Nonsense mutation around 237092, 237257, 237362.", "psc": { "uniref90_id": "UniRef90_Q50290", "gene": "mte1", "product": "Type II methyltransferase M.MpnI", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_Q50290", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50290", "EC:2.1.1.72", "UniRef:UniRef50_Q50290", "GO:0003677", "GO:0009007", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_Q50290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50290" ], "product": "Type II methyltransferase M.MpnI" } }, "id": "CNOCLNGIAL_459", "locus": "GCA000143945_00424" }, { "type": "cds", "sequence": "contig_1", "start": 237119, "stop": 237259, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTCCAAGTGCAGTCTAACTTTAAACTGTATGACCCCAAAAGCGTTAACTTTGTTAAAAAGTATGGTCAACTGTTTCAAAAAGTACCGGGAATTTCCTGGTTTACAAACCTCAAGAAAAACCAACAACCAGCTTGA", "aa": "MLFQVQSNFKLYDPKSVNFVKKYGQLFQKVPGISWFTNLKKNQQPA", "aa_hexdigest": "8287a83dc61059c650dec79e1dd78010", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5436.289600000001, "isoelectric_point": 10.097054481506348 }, "id": "CNOCLNGIAL_460", "locus": "GCA000143945_00425" }, { "type": "cds", "sequence": "contig_1", "start": 237374, "stop": 237574, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGGTGCCATTAACCTTTCTCAATTGCTTTAATCCCAAACAGTTTGAGCTAATTGATTGTCTAGCCAATCCCTACGCTACCCTAGACACATTAAAAACTAATGCTTATGTGAGATCACACCACGGTGATGTGAGGAATGTGAAGGGTAAGCGAAGGTATGTTAGGGTAGTGATTAAACAACGTCAAAATGTGATTTAA", "aa": "MGVPLTFLNCFNPKQFELIDCLANPYATLDTLKTNAYVRSHHGDVRNVKGKRRYVRVVIKQRQNVI", "aa_hexdigest": "741a38653363bee739e4e54424ad7a9f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7660.864300000004, "isoelectric_point": 10.024140739440917 }, "id": "CNOCLNGIAL_461", "locus": "GCA000143945_00426" }, { "type": "cds", "sequence": "contig_1", "start": 237668, "stop": 238462, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCAAAATATTTTTGGGCAGCAGCTTTTTTGGTGTTGCCACCTTACTTAGTGCTTGTGGTACTGGTCGCTTTGATCAAATCGATGATGGCAAAATTAAGCTTGCTGTTGTCACATCCCCTAGTGCCGCTAGTTCACTGCAAACCTTATTAGATCAATACAACAACACTAAAGCACCAGCTGATTATCCAGTGGAAGTAGTTTCACTAGACAGTACTGGGAGCTATACTAAAGGGAAATTTGATACCCAAAAACGGTTAGCTGCTAAAGATAAAAATAACTTTTATAACCTAACCTTTAACTACCCCGACTTAGTTTCATCTTTAGCAATTAACGGAATGGAACTCAACCTTGATGGGGTTAATGTTTCTAACTTCGAACAGTCTTTCTTAGACTTCAACAAGAACATTAGTGGCGTACGCAAACCCGGCATTTACGCCTTACCAGCTACCATGAGTGGTGAAGTTTTGGTTTTAAATGGGCCAGTTCTCCACTACATCTTGAACAGTGCCAAGAAGAAGGATGGTACCGAATCAAAAATTAAAACTGCTCAATTAAAAACAGCTTCAACTCAGGCAGAAGTTAAGGGTACTATGACCATGGCTAGTGATGAACAAACCACAAAGCTTTGGACTAATATTCAAAACGCCGCTAAGGAAAATGCCGATAACGGTACAACTGAGAAAGCGGAAAAAACAGTTTCCGCTAGTTCGTTGCAATTAAAAAATACGAATAAAACCACCGAAGGTACCCTAAAAATTGGTACTGATGACAACACTAAAAATCTTTGA", "aa": "MKFKIFLGSSFFGVATLLSACGTGRFDQIDDGKIKLAVVTSPSAASSLQTLLDQYNNTKAPADYPVEVVSLDSTGSYTKGKFDTQKRLAAKDKNNFYNLTFNYPDLVSSLAINGMELNLDGVNVSNFEQSFLDFNKNISGVRKPGIYALPATMSGEVLVLNGPVLHYILNSAKKKDGTESKIKTAQLKTASTQAEVKGTMTMASDEQTTKLWTNIQNAAKENADNGTTEKAEKTVSASSLQLKNTNKTTEGTLKIGTDDNTKNL", "aa_hexdigest": "42ada610b3fb073b460af693806b07d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28508.791700000016, "isoelectric_point": 8.71536808013916 }, "id": "CNOCLNGIAL_462", "locus": "GCA000143945_00427" }, { "type": "cds", "sequence": "contig_1", "start": 238864, "stop": 239682, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAACATTAAAAAAGGTCGCGCTGATTTTAGTAACTTCTTTAACCACAATTCAAAAACTTTCCAAAGTCTAAGAGATATTTTCAACAGCTTTAAGCCTTTAATTGACCAAAAGGGTTTAATTAATAACAAACACTTTGATACTCCAGTGAATAATTACGCTAAATTTCACCAATTGGCATTCTATGTATCATCGACAGCTCGCTTTCCTTACTCGTTTGCCAAAGATAATGTAAAACGCTTAATTATTGGTAAACGCGAATTGGAAGTAAACCCTAAATCAATGTTTGCCATTAAGAAGGAAAACGGCAATAACGGTAACAGCAACCTCTTAGGTAAAGTTGCACTAGATAACAATAAATCTATTGAACTTTACGAAAATAATATTCCTAACGGTAAAACTGATGCCATCTTAATTAAGAACCAAACTTTAATTAGTGCGTTGAAGAATCCAAAACAGACAAAGAGCTCTCAAAGATCTTCTCAGTCTACAAACACCCAAAATGATGCCATTTGTTACTTAGCCTTCAACAGCAAGATTAGACCAGACGATAAAGACATTTTCCTATTAGACAAATTTGGTGAAAAATTTGTCACCGCAATTGTTAACTTTGAAGAAAAAACGGAGGTCAAAATTAATACGCTCCAAGAAAAGGAAGCTGTTGTTTTACCAGCACCACAAAAGTTTAAACCTACTGATCCAAAATCAGTGGCACTTGTTCAAGGTCCAAGTTTAATCGGTATTCACGCTAACGCTAACGAAGACCGTTCAACCATTAAGTTTCTCAACTGGTACCTTAATGCTGAAGTTGATTGA", "aa": "MFNIKKGRADFSNFFNHNSKTFQSLRDIFNSFKPLIDQKGLINNKHFDTPVNNYAKFHQLAFYVSSTARFPYSFAKDNVKRLIIGKRELEVNPKSMFAIKKENGNNGNSNLLGKVALDNNKSIELYENNIPNGKTDAILIKNQTLISALKNPKQTKSSQRSSQSTNTQNDAICYLAFNSKIRPDDKDIFLLDKFGEKFVTAIVNFEEKTEVKINTLQEKEAVVLPAPQKFKPTDPKSVALVQGPSLIGIHANANEDRSTIKFLNWYLNAEVD", "aa_hexdigest": "e8b0f7f8f5802afbd4542f9c2bb03eda", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30841.673300000024, "isoelectric_point": 9.520384788513184 }, "id": "CNOCLNGIAL_463", "locus": "GCA000143945_00428" }, { "type": "cds", "sequence": "contig_1", "start": 239740, "stop": 240102, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGTTTAAAAAAGTATTAGAAAAATCCAAGCAAAACATAAAAGCGACCTCTAAGGAAGGAGAGCAAAATCAAGGCAAGAAAGGTGATGGTGCACAGAACCAAGGTAAGAAGGGCGATGGAGCTCAAAATGGTAAGAATGATAAAGCAAAGCACAATGGTTTTGTCGATATTGCCGTCAGCCAATTTTTAAACGGTTCTGTCGCTAAGTTTGTGGAACCAGCTGACTTTTTAGGTATTCGCTTACGTGATGCCGTTAAGAGTACCTTTAACGCTGCCATTAACAACACCTCAATTAACTTTGACAAGTTCATTCAGTACATTAACGATTCTTTAGGTAGCGACTTTAAGCAAAGACAGTAG", "aa": "MPFKKVLEKSKQNIKATSKEGEQNQGKKGDGAQNQGKKGDGAQNGKNDKAKHNGFVDIAVSQFLNGSVAKFVEPADFLGIRLRDAVKSTFNAAINNTSINFDKFIQYINDSLGSDFKQRQ", "aa_hexdigest": "7483ec5773002d78fc25461d4bb3f3d0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13166.572299999983, "isoelectric_point": 9.697608375549319 }, "id": "CNOCLNGIAL_464", "locus": "GCA000143945_00429" }, { "type": "cds", "sequence": "contig_1", "start": 240232, "stop": 241032, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTAAGTATGGTGCCATTGTTTTCAGTGGTCTTTTAGGAGTCTCTGCCATTTTAGCTGCTTGTGGTACACGCGGTAAATTTGACCAAATTGATGATGGCAAAATTAAGCTAGCATCTTCGTTAACTTCCAAAGGTGCCGCTAATGCTTTACAAGCAATTGTGAAGAAGTATAACGAGGTTAAAAAGCCTGGTGATTACCCAATTGAAATAACCCAAATTGCGGGTGGTTACGACCAAGCACGCGTTGACTTACAATCACGGGTTGGTGTTAAGGATAAAACCAATTTTTACAACCTCATTTTGAACTATCCCGATGTAGTTTCAGTGCTAGCACGTAACCAAATGGAACTGCCATTTGATGGTGTTGATGTTTCTAAAATTTCACCTAACTTTTTAAATTTTAACGAACGTATTAGCGGAGTTTCTAAAAAGGCTAACTATGCCATTCCGGTCTCTGTTTCGACTGATATTTTAGTGCTCAATGGACCAGTTTTGCACTACATTTTAAACAGTGCTAAAGGTGAAAGTAAGGGCGCACAAAAAGATAACAAGAGTGCTGAAGTTCAAAGAAAAAGTACAGGTCAAAAGACCGTTACCCAACCTTTAACTATTGCAACTGATTCTGCTACAAACGGTCTTTGGAAAAAGATTGAAGATGCTGCAAAAGTCAGCGGTAAAAAGAAAGAAGAGAAAAAGAGTACACGCAGTAAAAGAGCCACAGAAGGCACACAAACCACCAAAGAAAATACGGGTGGTGATGCTGCAACAAGTGATACCAAAATTAAAGAATCTTGA", "aa": "MKFKYGAIVFSGLLGVSAILAACGTRGKFDQIDDGKIKLASSLTSKGAANALQAIVKKYNEVKKPGDYPIEITQIAGGYDQARVDLQSRVGVKDKTNFYNLILNYPDVVSVLARNQMELPFDGVDVSKISPNFLNFNERISGVSKKANYAIPVSVSTDILVLNGPVLHYILNSAKGESKGAQKDNKSAEVQRKSTGQKTVTQPLTIATDSATNGLWKKIEDAAKVSGKKKEEKKSTRSKRATEGTQTTKENTGGDAATSDTKIKES", "aa_hexdigest": "34a1cec5e0ebd9e592aebfa5092343da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28638.207500000026, "isoelectric_point": 9.57415142059326 }, "id": "CNOCLNGIAL_465", "locus": "GCA000143945_00430" }, { "type": "cds", "sequence": "contig_1", "start": 241192, "stop": 242286, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_Q50288" ], "nt": "GTGTTAGGTGTTGATAATTCACCTAATGCTTTACTATCTTCGGTGTTTGCTGCTGGTAACAGTGACTATAACAACTTCTTTTATAAGGTGCAAAATGGTCGGGCTGACTTTAGCAACTTTAACAATAAAGGCAGTTCTTATCAAAACCTTAAAAATGTCTTTAACGACTATAAGAACCTAATTGCACAAAATGGTCTTTATGTAAATAAAGGCGGCTCTTACTCTTCAAACTTCCAAAAGTTTCACCAGTTAGCTTACAGCATTTCCTCTACTTCTGGTTTTGCTTATTCGTTTGCTGGACAAAATTCCAAACGTTTTAAATTTACTGATGATGGTACTTTTGTTGAGTATCCTAGTTATACAACTGAAGTTAATGCACCAGAAAGTAATAATGGCAATGATGGAAAGCAACAAGGTCAAAGTGATCAAGGAAACCTTTTAGGAACTTTTGAAGTTGTAGATAAAAGTACTAGTGATATAAAGGTGAAACCTAAAACTCAAGCGGAATCAAAGAAAAGTAGTGATAGTAAACAAACTGCAAACACAGGCAAAGGCAGTAATTCAAAACAACAAACTCCGAAGAAAACTATTTCGCTTTATAAAACCAAAATTCCTCAAGATAAAACAGAAAACGTTGACGCTTTTTTAGTTACTGATTCAGAATTAATTAGCAAACTTGAAAAAGCTAAAAATAAAAAGGAAGAAACTAAAGCTTCAGGTAAATCTGCATCTGCACGTGTGGCAGTTCAAGCAACTCAAAAGAAACAGTCTAACGAAAAGCAGATAGTTGGTTACACTACTACATCCGCATTAAGCGAAGACGGCAAACACATTTTTAAATTGGGTAAACTCAACAGCGAGAACTATGAGCGCAAAATCATTGTTGGTGCTACTGTAGAAACACTAGAACAATCCACTACCTTGCAAAGTGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAGTACAAGGATAGTGATCAAAAGAGAGTTATGATCACCCAAGGTCCTAACCTAATAGGGGTTCATGCTAATACTAAAGAAAATGAAGAGACTAAGAAATTTGTTAACTGGTTTTTAAATAAAACCGAAAGTTGA", "aa": "MLGVDNSPNALLSSVFAAGNSDYNNFFYKVQNGRADFSNFNNKGSSYQNLKNVFNDYKNLIAQNGLYVNKGGSYSSNFQKFHQLAYSISSTSGFAYSFAGQNSKRFKFTDDGTFVEYPSYTTEVNAPESNNGNDGKQQGQSDQGNLLGTFEVVDKSTSDIKVKPKTQAESKKSSDSKQTANTGKGSNSKQQTPKKTISLYKTKIPQDKTENVDAFLVTDSELISKLEKAKNKKEETKASGKSASARVAVQATQKKQSNEKQIVGYTTTSALSEDGKHIFKLGKLNSENYERKIIVGATVETLEQSTTLQSEEAIVLAAPGKYKDSDQKRVMITQGPNLIGVHANTKENEETKKFVNWFLNKTES", "aa_hexdigest": "7c378696e96dc07d36c06ef8d4558876", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 240892, "stop": 242589, "observations": { "mutation": [ 241030, 241099, 242284 ], "stop": [ 241030, 241099, 242284 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50288", "query_cov": 1.0, "subject_cov": 0.45614035087719296, "identity": 0.995, "score": 677.0, "evalue": 9.39e-239, "gene_start": 1, "gene_end": 364, "reference_start": 321, "reference_end": 684, "reference_sequence": "MKFKYGAIVFSGLLGVSAILAACGTRGKFDQIDDGKIKLASSLTSKGAANALQAIVKKYNEVKKPGDYPIEITQIAGGYDQARVDLQSRVGVKDKTNFYNLILNYPDVVSVLARNQMELPFDGVDVSKISPNFLNFNERISGVSKKANYAIPVSVSTDILVLNGPVLHYILNSAKGESKGAQKDNKSAEVQRKSTGQKTVTQPLTIATDSATNGLWKKIEDAAKVNGKKKEEKKSTRSKRATEGTQTTKENTGGDAATSDTKIKESWGAYQEVEGGLKGYQFKAIVFENWHDLIDFSTRVAKSFSKVKDNSNKKGNEIQGVLGVDNSPNALLSSVFAAGNSDYNNFFYKVQNGRADFSNFNNKGSSYKNLKNVFNDYKNLIAQNGLYVNKGGSYSSNFQKFHQLAYSISSTSGFAYSFAGQNSKRFKFTDDGTFVEYPSYTTEVNAPESNNGNDGKQQGQSDQGNLLGTFEVVDKSTSDIKVKPKTQAESKKSSDSKQTANTGKGSNSKQQTPKKTISLYKTKIPQDKTENVDAFLVTDSELISKLEKAKNKKEETKASGKSASARVAVQATQKKQSNEKQIVGYTTTSALSEDGKHIFKLGKLNSENYERKIIVGATVETLEQSTTLQSEEAIVLAAPGKYKDSDQKRVMITQGPNLIGVHANTKENEETKKFVNWFLNKTESWEVKGNGKDSQTTKSLTPAQYFAESASYILPLKETVEKDHKEQTNKNTYVAKALEMLKEVSENKSVSYSDPSDFRSGRFRDALGANFNATINSKANFEKFFQGFKAALGSDFDK" }, "paralog": false, "identity": 0.9911504424778761, "score": 1096.0, "evalue": 0.0, "description": "Internal stop codon at 241030, 241099, 242284. Nonsense mutation around 241030, 241099, 242284.", "psc": { "uniref90_id": "UniRef90_Q50288", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50288", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "CNOCLNGIAL_466", "locus": "GCA000143945_00431" }, { "type": "cds", "sequence": "contig_1", "start": 242371, "stop": 242598, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGCTAAAGGAAACTGTTGAAAAAGATCATAAAGAACAAACTAATAAAAACACCTATGTTGCCAAAGCTTTAGAAATGCTCAAGGAAGTTAGTGAAAACAAATCAGTCAGCTATAGTGATCCGAGTGATTTTAGAAGTGGTAGATTCCGTGATGCACTTGGTGCTAACTTTAATGCCACTATTAATTCAAAAGCAAATTTTGAAAAGTTTTTTCCAAGGTTTTAA", "aa": "MPLKETVEKDHKEQTNKNTYVAKALEMLKEVSENKSVSYSDPSDFRSGRFRDALGANFNATINSKANFEKFFPRF", "aa_hexdigest": "6e12b8771c706f48823bb9ff24fbce22", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8651.582399999996, "isoelectric_point": 9.003348350524902 }, "id": "CNOCLNGIAL_467", "locus": "GCA000143945_00432" }, { "type": "cds", "sequence": "contig_1", "start": 242734, "stop": 242913, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGAGATTAAAACTTACAAAATCAAAGATATTTGTGATATTACACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGA", "aa": "MEIKTYKIKDICDITRGRVISKLDIKKDPGVFPVYSAATNNDGEFGRINSYDFDGEYVT", "aa_hexdigest": "5e30dbd3b8714ccd24b8094a741d69d4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 61, "aa_cov": 0.9661016949152542, "hmm_cov": 0.9836065573770492, "evalue": 1.6521725131306922e-10, "score": 36.86839294433594, "start": 3, "stop": 59 } ], "seq_stats": { "molecular_weight": 6708.495900000002, "isoelectric_point": 5.161514854431151 }, "id": "CNOCLNGIAL_468", "locus": "GCA000143945_00433" }, { "type": "cds", "sequence": "contig_1", "start": 242894, "stop": 242989, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGA", "aa": "MVNMLLEQLTVMEGLFFIVMVNLASQIFVVF", "aa_hexdigest": "4165f2c157215872847f65036bab0f87", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3577.3857, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_469", "locus": "GCA000143945_00434" }, { "type": "cds", "sequence": "contig_1", "start": 243103, "stop": 243444, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HM83", "UniRef:UniRef90_A0AB33HM83", "PFAM:PF01420.25" ], "nt": "ATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGGGCGCGCAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTTAATCAAGAAAATATCCGAAAAATTTATGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACACATTAAAGATTGCGACTTTGATGGTGAATACATCACTTGA", "aa": "MAEIPIDFPPLKIQEKIATILDTFTELRARKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGHIKDCDFDGEYIT", "aa_hexdigest": "9e0fab2716bb870ba94d3a5e0a852fcd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 243133, "stop": 243747, "observations": { "mutation": [ 243442 ], "stop": [ 243442 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HM83", "query_cov": 0.9823008849557522, "subject_cov": 0.45491803278688525, "identity": 0.883, "score": 197.0, "evalue": 7.77e-62, "gene_start": 11, "gene_end": 113, "reference_start": 1, "reference_end": 111, "reference_sequence": "MQYQEKIATILDTFTELSAELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYITWTTNGYAGVVFYRNGKFNASQDCGVLKVQNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.9245283018867925, "score": 389.0, "evalue": 1.37e-139, "description": "Internal stop codon at 243442. Nonsense mutation around 243442.", "psc": { "uniref90_id": "UniRef90_A0AB33HM83", "uniref50_id": "UniRef50_A0AB33HM83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HM83", "UniRef:UniRef50_A0AB33HM83" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HM83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HM83" ] } }, "hypothetical": true, "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.5132743362831859, "hmm_cov": 0.9838709677419355, "evalue": 4.0518338202620285e-18, "score": 61.630435943603516, "start": 56, "stop": 113 } ], "seq_stats": { "molecular_weight": 13108.738399999984, "isoelectric_point": 5.838808250427246 }, "id": "CNOCLNGIAL_470", "locus": "GCA000143945_00435" }, { "type": "cds", "sequence": "contig_1", "start": 243631, "stop": 243798, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00190F3D87", "UniRef:UniRef90_UPI00190F3D87" ], "nt": "ATGGCTGAAATCGAACTCTCTTTCCCACCTCTAGAAATCCAAGAAAAGATTGCCGATATTCTCTTTGCTTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "bbbc1991845ad67ac2a240d1c026608d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 243484, "stop": 243843, "observations": { "mutation": [ 243796, 243817 ], "stop": [ 243796, 243817 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00190F3D87", "query_cov": 1.0, "subject_cov": 0.46218487394957986, "identity": 1.0, "score": 114.0, "evalue": 1.06e-31, "gene_start": 1, "gene_end": 55, "reference_start": 50, "reference_end": 104, "reference_sequence": "GKCNASQDCGVLKVKNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.8907563025210085, "score": 207.0, "evalue": 8.52e-71, "description": "Internal stop codon at 243796, 243817. Nonsense mutation around 243796, 243817.", "psc": { "uniref90_id": "UniRef90_UPI00190F3D87", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00190F3D87", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00190F3D87", "EC:3.1.21.3", "UniRef:UniRef50_UPI00190F3D87", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00190F3D87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00190F3D87" ], "product": "restriction endonuclease subunit S" } }, "id": "CNOCLNGIAL_471", "locus": "GCA000143945_00436" }, { "type": "cds", "sequence": "contig_1", "start": 243849, "stop": 244274, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_308010181.1", "SO:0001217", "UniParc:UPI0027E159D2", "UniRef:UniRef100_UPI0027E159D2", "UniRef:UniRef50_A0AB38W7Y3", "UniRef:UniRef90_UPI0027E159D2" ], "nt": "GTGGTTTTCGTTGCCTTGGGTGTTATTCCCAGTAGTCTTATCATTCCTTCACCCCGCCCCCGTTCAGGGCTGGAGGTTGGCTTTGTCGAGGGTGGATCATTTGGAGCCGCCTGTAGTCGTCGTCTGACTGCCACCGAGCGTGGCTTGGACGTTGGCTTTGAGACCAACGCCGACATTCGAGAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTGAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTGCCATACCTGTACCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCGAGCACCGACCCCGCGAGGTTGAACCCGGTGGAGAAGCGGGTGAGCGTGGGGGAGAAGTGGGATCAGTTTGTGGTTTGCTGAACTAA", "aa": "MVFVALGVIPSSLIIPSPRPRSGLEVGFVEGGSFGAACSRRLTATERGLDVGFETNADIRERREKGTRTTCVRTSSAGRPRAGLRRGYIIHAPVVIAIPVPIPNPGNIIQHLVEHRPREVEPGGEAGERGGEVGSVCGLLN", "aa_hexdigest": "90cc5db1e0963df56dd341c84f152ab9", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0027E159D2", "ncbi_nrp_id": "WP_308010181.1", "uniref100_id": "UniRef100_UPI0027E159D2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0027E159D2", "RefSeq:WP_308010181.1", "UniRef:UniRef100_UPI0027E159D2" ] }, "ips": { "uniref100_id": "UniRef100_UPI0027E159D2", "uniref90_id": "UniRef90_UPI0027E159D2" }, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14928.934299999983, "isoelectric_point": 9.585820198059082 }, "id": "CNOCLNGIAL_472", "locus": "GCA000143945_00437" }, { "type": "cds", "sequence": "contig_1", "start": 244299, "stop": 244466, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAGTCAAAACCAGGGTTCTACAACAACTACCTCAGCGGGCAATCCCGATTCTTTAGTTACTGATAAAGTTGATCAAAAAGGTCAGGTGCAAACAAGTGGTCAAAATTTAAGTGAGACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACATCCGACTGA", "aa": "MGSQNQGSTTTTSAGNPDSLVTDKVDQKGQVQTSGQNLSETNYTNLPPNLTPTSD", "aa_hexdigest": "93d85bf968dc2fb08c123730dd6d176c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5726.9822, "isoelectric_point": 4.1962182998657225 }, "id": "CNOCLNGIAL_473", "locus": "GCA000143945_00438" }, { "type": "cds", "sequence": "contig_1", "start": 244548, "stop": 244808, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTTAATAAGAGTGGCGAAAATAACGAAAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGCTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGA", "aa": "MLVNKSGENNEKFQATDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGLKFPNKIMIQRLC", "aa_hexdigest": "eb9d38ae99f201314af7b3da8d2accf4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9556.690999999993, "isoelectric_point": 8.818066215515138 }, "id": "CNOCLNGIAL_474", "locus": "GCA000143945_00439" }, { "type": "cds", "sequence": "contig_1", "start": 245051, "stop": 245311, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_369122719.1", "SO:0001217", "UniParc:UPI0034DD7934", "UniRef:UniRef100_UPI0034DD7934", "UniRef:UniRef50_UPI0034DD7934", "UniRef:UniRef90_UPI0034DD7934", "PFAM:PF03257.18" ], "nt": "GTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGACCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTACCCAATCCGACAAAGTTAGACGACGATCTTAA", "aa": "MESVWDLKGVWADQAQSDSQGSTTTATGATLPEHPNALAFQVSVVEASAYKPNTSSDQTQSTNSSPYLHLVKPKKVTQSDKVRRRS", "aa_hexdigest": "240f13c025b72f1e63854f0286dc5eb8", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0034DD7934", "ncbi_nrp_id": "WP_369122719.1", "uniref100_id": "UniRef100_UPI0034DD7934", "db_xrefs": [ "SO:0001217", "UniParc:UPI0034DD7934", "RefSeq:WP_369122719.1", "UniRef:UniRef100_UPI0034DD7934" ] }, "ips": { "uniref100_id": "UniRef100_UPI0034DD7934", "uniref90_id": "UniRef90_UPI0034DD7934" }, "psc": { "uniref90_id": "UniRef90_UPI0034DD7934", "uniref50_id": "UniRef50_UPI0034DD7934" }, "pscc": { "uniref50_id": "UniRef50_UPI0034DD7934", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD7934" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 38, "aa_cov": 0.43023255813953487, "hmm_cov": 1.0, "evalue": 1.2993797001614837e-15, "score": 53.37580871582031, "start": 46, "stop": 82 } ], "seq_stats": { "molecular_weight": 9335.141099999997, "isoelectric_point": 8.076035118103025 }, "id": "CNOCLNGIAL_475", "locus": "GCA000143945_00440" }, { "type": "cds", "sequence": "contig_1", "start": 245346, "stop": 245540, "strand": "+", "frame": 3, "gene": null, "product": "Putative adhesin P1-like protein MPN_203", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50284", "UniRef:UniRef50_UPI0027E1A8D0", "UniRef:UniRef90_Q50284", "UniRef:UniRef90_UPI0027E1A8D0" ], "nt": "ATGCTCAAATCATTTGGTACAGAAAATTTCACCCAGCCCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGGTACCACGCAGGGCTCAAGCACCACCAATCAACTGTCACCCGTTCAACGGGTGAGTGGGTGGACCGCTAA", "aa": "MLKSFGTENFTQPQPQPQALKTTTPVFGTSSGNLGSVLSGGGYHAGLKHHQSTVTRSTGEWVDR", "aa_hexdigest": "a8395a495eecbe98e740e258af41e552", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E1A8D0", "query_cov": 0.890625, "subject_cov": 1.0, "identity": 0.982, "score": 119.0, "evalue": 3.97e-34, "valid": true, "uniref50_id": "UniRef50_UPI0027E1A8D0" }, "pscc": { "uniref50_id": "UniRef50_UPI0027E1A8D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027E1A8D0" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 245157, "stop": 245540, "observations": { "mutation": [ 245175, 245262 ], "stop": [ 245175, 245262 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50284", "query_cov": 1.0, "subject_cov": 0.5039370078740157, "identity": 1.0, "score": 134.0, "evalue": 1.68e-39, "gene_start": 1, "gene_end": 64, "reference_start": 64, "reference_end": 127, "reference_sequence": "MLWPFRWVWWKRVLTSQTRAPAKPNPLTVPPTCTWWSLRKLPNPTKLDDDLKNLLDPNEVRARMLKSFGTENFTQPQPQPQALKTTTPVFGTSSGNLGSVLSGGGYHAGLKHHQSTVTRSTGEWVDR" }, "paralog": false, "identity": 0.9763779527559056, "score": 263.0, "evalue": 1.44e-92, "description": "Internal stop codon at 245175, 245262. Nonsense mutation around 245175, 245262.", "psc": { "uniref90_id": "UniRef90_Q50284", "product": "Putative adhesin P1-like protein MPN_203", "uniref50_id": "UniRef50_Q50284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50284", "UniRef:UniRef50_Q50284" ] }, "pscc": { "uniref50_id": "UniRef50_Q50284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50284" ], "product": "Putative adhesin P1-like protein MPN_203" } }, "id": "CNOCLNGIAL_476", "locus": "GCA000143945_00441" }, { "type": "cds", "sequence": "contig_1", "start": 245652, "stop": 245924, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574907.1", "SO:0001217", "UniParc:UPI0001DD64E7", "UniRef:UniRef100_A0AB38WAH1", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0AB38WAH1" ], "nt": "ATGAAATTGATTAAATTAGCGAGGTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTTCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTGTTGACACCTTTAAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAAAAGGAGGTATTACTTAATGAAAGAAAAAACTCCCTTTAA", "aa": "MKLIKLARFHRFLKQDQLQLVSLFSQNFLQVLLTPLNKPVNLFSENTTMKTNKKYAFAALLMQAQAKLPNSFKFFLSKKEVLLNERKNSL", "aa_hexdigest": "c706c9c9d50737294f2b4bb24c0c5725", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64E7", "ncbi_nrp_id": "WP_014574907.1", "uniref100_id": "UniRef100_A0AB38WAH1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64E7", "RefSeq:WP_014574907.1", "UniRef:UniRef100_A0AB38WAH1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38WAH1", "uniref90_id": "UniRef90_A0AB38WAH1" }, "psc": { "uniref90_id": "UniRef90_A0AB38WAH1", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10546.470099999991, "isoelectric_point": 10.505590629577636 }, "id": "CNOCLNGIAL_477", "locus": "GCA000143945_00442" }, { "type": "cds", "sequence": "contig_1", "start": 246070, "stop": 246348, "strand": "+", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_410", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572", "UniRef:UniRef90_P75572" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTAAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAGCAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGCTTGGACAAAATTGATCCATCTAAATAG", "aa": "MNTEYVTRKELNEYKDSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPSK", "aa_hexdigest": "05e342d677ef9c6d60d35fd88b1e7dc3", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 245902, "stop": 246348, "observations": { "mutation": [ 245983 ], "stop": [ 245983 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75572", "query_cov": 1.0, "subject_cov": 0.6216216216216216, "identity": 0.9890000000000001, "score": 111.0, "evalue": 1.31e-29, "gene_start": 1, "gene_end": 92, "reference_start": 57, "reference_end": 148, "reference_sequence": "MKEKTPFKNEKEFHEMVKKTKKGTFSGWYIIDKDNNSVEFSGKFNRQFKLNKPVITVNTEYVTRKELNEYKDSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPSK" }, "paralog": false, "identity": 0.9864864864864865, "score": 225.0, "evalue": 8.35e-77, "description": "Internal stop codon at 245983. Nonsense mutation around 245983.", "psc": { "uniref90_id": "UniRef90_P75572", "product": "UPF0134 protein MPN_410", "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75572", "UniRef:UniRef50_P75572" ] }, "pscc": { "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572" ], "product": "UPF0134 protein MPN_204" } }, "id": "CNOCLNGIAL_478", "locus": "GCA000143945_00443" }, { "type": "cds", "sequence": "contig_1", "start": 246809, "stop": 247063, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGATAACACTGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCTGCGCGAACCGAGGCCACCTTGACCACCATCACCTTTGCGCTGCGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCAACGCGTGATGCCAACGGCCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGCAGTGCACCCCAACAGTGA", "aa": "MRDNTAKGITAGSGSQQTTYDPARTEATLTTITFALRRYDLAGRALYDLDFSKLNPQTPTRDANGQITFNPFGGFGLSGSAPQQ", "aa_hexdigest": "d5c6f52271a63801051801098148bfbf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 56, "aa_cov": 0.6547619047619048, "hmm_cov": 0.8928571428571429, "evalue": 1.3241711984473717e-07, "score": 26.69129753112793, "start": 29, "stop": 83 } ], "seq_stats": { "molecular_weight": 9031.830799999998, "isoelectric_point": 8.137860298156738 }, "id": "CNOCLNGIAL_479", "locus": "GCA000143945_00444" }, { "type": "cds", "sequence": "contig_1", "start": 247094, "stop": 247594, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCCAAGACCCCACCGATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGTGTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGTTCGACCAATTCCGACACCAAAGTCAAGGCTTTAAAAATAGAGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGCTGCAGTTAGAAAAAAATGATCTCGCCAACGCTCCCATTAAGCGGGGTGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGA", "aa": "MAQDPTDPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGSTNSDTKVKALKIEVKKKSDSEDNGQLQLEKNDLANAPIKRGEESGQSVQLKADDFGTAPSSSGSGGNSNPGSPTP", "aa_hexdigest": "7a57e927cdc38ee00ed47581fc7b4353", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17354.60639999997, "isoelectric_point": 6.317733573913575 }, "id": "CNOCLNGIAL_480", "locus": "GCA000143945_00445" }, { "type": "cds", "sequence": "contig_1", "start": 247556, "stop": 248080, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCCGACTTGGTAACATGGCCATTGCCACCAAACACCAAAAGCGCTTGCCCGAACCAGGTGAGGTTGCCAATGTTGGCGAAGTACGCTTCAAACGGGAGGGCGATGGGGGCCGACTTGTCGGAGTCAGCTTCCTTTTGAAAGCCCTGCTTGGTGTTCGAAGTATTACTTTCATCAAACCCGGTTTGGGTATTATCCGCGACCGCTTGGCCCTGGTCAAACCACTCGGGGTGGCGGCGCGGGTTGAAGAACCCGGTGGTTTGGAACAAAACATCGCGCGCCTTTCAGTCCCACAAACCGTGGTGGTTTCACTTGGGCGTTCTTCAACTGGGCGGATAGTTCGCGGTCATGGCCTTGGGATCCAAGTGATCAACGCGGTCAATGGCGGTACGGTTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGATCATTTGGGGAGGTCCTTGTGAATTTGCTCAGTCGCAAGCCACGGCCTTCAGGGGGTGGGGGAACCGGGGTTGGAGTTGCCGCCTGA", "aa": "MGRLGNMAIATKHQKRLPEPGEVANVGEVRFKREGDGGRLVGVSFLLKALLGVRSITFIKPGLGIIRDRLALVKPLGVAARVEEPGGLEQNIARLSVPQTVVVSLGRSSTGRIVRGHGLGIQVINAVNGGTVARIRRIVQNQDRGGSFGEVLVNLLSRKPRPSGGGGTGVGVAA", "aa_hexdigest": "135e833192f0291be6cf0b3ab844164a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18147.94999999998, "isoelectric_point": 11.777165031433107 }, "id": "CNOCLNGIAL_481", "locus": "GCA000143945_00446" }, { "type": "cds", "sequence": "contig_1", "start": 248773, "stop": 248997, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCCGATTGCCGTTTTACCTATTGCGGGTATTTTTTTGGGTGTTGGTGATGCAATTGCCGGAAATGCAGGTGATTTAACTGGTCTGCGTTATTTCGGACTTTTCATTAAAAATGGTGGTGATGTTGTTTTTGCTAACCTACCCATTTTGTTTGCTATTGCTATAGCAATAACGTTCTCACAAGACGCTGGGGTCGCCGGATTTTCGGCCTTTGTCTTTTGA", "aa": "MIPIAVLPIAGIFLGVGDAIAGNAGDLTGLRYFGLFIKNGGDVVFANLPILFAIAIAITFSQDAGVAGFSAFVF", "aa_hexdigest": "14e5d9a47c587e87911697fbcf380fdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7518.7656, "isoelectric_point": 4.138697242736816 }, "id": "CNOCLNGIAL_482", "locus": "GCA000143945_00447" }, { "type": "cds", "sequence": "contig_1", "start": 249004, "stop": 249330, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACGGGTTTATGAGCTCGTTAATATTGCCTTTTGATAAAGCAGGCAAAATTATTACTGACACTTCGACTCCAATTGCTGGTTTTAAAGTACTTTACTACAAGAGTGTCCCTGTTCATGCTATTGCTACAACCTTGGGACTGCGAACATTATCAACCTCAGTATTCGGTGGGATTATTGTAGGTGCCTTAACTTCAGTACTTTACAAGAAGTTTTATGCCATTAGGTTACCTGATGTAATTGGATTCTTTAGCGGTACTCGTTTTGTCCCTATTATTTGTTTTGTGGTAGCGATTCCTGTTGCGTTAATCTTGTTAATGATCTGA", "aa": "MNGFMSSLILPFDKAGKIITDTSTPIAGFKVLYYKSVPVHAIATTLGLRTLSTSVFGGIIVGALTSVLYKKFYAIRLPDVIGFFSGTRFVPIICFVVAIPVALILLMI", "aa_hexdigest": "ae6474d1b9b520b7a4a9996aa1460cab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11629.966399999988, "isoelectric_point": 9.843049049377441 }, "id": "CNOCLNGIAL_483", "locus": "GCA000143945_00448" }, { "type": "cds", "sequence": "contig_1", "start": 249820, "stop": 250278, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTCTGAACGGTTCTGGTATTTTAGGTAAAAAATTGTTACTAAGTGATGGTAATGTTTACACTATTACCTTTAAAACCTTTGCCAACACTACTCCAATTGCTTGGAGTAAAGGTGCACAGGCAGTGTTACCTTTAAATGCTAGCTCTACAATCGTTAACAATCCAACCGCTTTAGCAGCAGCTACACAAAGTAATAATAACACTAACAACATTAAACTTTACCCGGTAAATTCCTTTAGGGTAGCTGTTGAAAGTTTAAATCCAGCGCAATACTCCCAGGGTAAATTCCCCTTCATGTTGTTTGGAATTCCAGCAGCTGGTGTAGCCATGATCTTGGCAGCACCTAAAGATAGAAGAAAGGAAGCAGCTTCGATTGTTGGTAGTGCAGCATTTACCAGCTTCTTAACTGGTATTACTGAACCATTTGAATTTACCTTCTTGTTCTTAGCGCCTTGA", "aa": "MGLNGSGILGKKLLLSDGNVYTITFKTFANTTPIAWSKGAQAVLPLNASSTIVNNPTALAAATQSNNNTNNIKLYPVNSFRVAVESLNPAQYSQGKFPFMLFGIPAAGVAMILAAPKDRRKEAASIVGSAAFTSFLTGITEPFEFTFLFLAP", "aa_hexdigest": "e731937ab6c91344ce87cd581481d22b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16049.296199999986, "isoelectric_point": 9.75440502166748 }, "id": "CNOCLNGIAL_484", "locus": "GCA000143945_00449" }, { "type": "cds", "sequence": "contig_1", "start": 250327, "stop": 251466, "strand": "+", "frame": 1, "gene": "ptsG", "product": "PTS system glucose-specific EIICBA component", "db_xrefs": [ "COG:COG2190", "COG:G", "EC:2.7.1.199", "GO:0005886", "GO:0008982", "GO:0009401", "GO:0016301", "GO:0090563", "SO:0001217", "UniRef:UniRef50_P47315", "UniRef:UniRef90_P75569" ], "nt": "ATGAACATCTTGGGTGCTAATGTGGGTCAAACCTTCTCTGGTTCCTTTATTGACTTTATTCTCTATGGTGCTTTACCAGATGGTCGTCGTTGGTTAGCAAACTCTTACTTAGTACCTATTATTGGACTGTTCTTAGCTGCCATCTACTTCCCAACCTTCTACTTCTTAATTAAGCACTTTAACTTGGCAACTCCAGGTCGTGGTGGTAAGTTAATTACCAAGAAGGAATACTTAGCAAGCAAAGCAGCCGCTAAAGCAGAGGGTGTAAGTGGTGTAGCAGAAAACTTTACCCAAACGCAAATTGAAGCGGGCATCTTACTCCAAGCTTACGGTGGCAAGGAAAACATTGTTGAACTAGGTGCATGTATTACCAAGTTACGGGTTACTGTTAAGAACCCCGAACTAGTTAAGGAAGAACCTATTAAAGAACTTGGAGCTGCTGGGGTAATGCGTACCACCCCAACCTTCTTTGTAGCCGTTTTTGGTACCCGCGCTGCCGTTTACAAATCTGCTATGCAGGACATTATCCAGGGCAAGGTTAACTGGGAAGCGTTGCAAAAGGTTATTAATACAGATCAACTAGCTGTTGAGCCTAAAGAAACCACTCCACCAAAGGAAGTAATGCCGGTGGTACAAGACGAAATTGTGATTCTGTCCCCAGTTAACGGTACCTTAAAATCACTCAATCAAGTACCCGATGAAACCTTTAAGCAAAAGCTAGTGGGTGAAGGTGTTGCCATTGTACCGAGCGATGGTCACTTTAAAGCCCCTGGTGAAGCAGGTGTGAAAACGGAGTTAGCTTTCCCTGGTGGTCACGCTTACATCTTTGACATTGATGGCATTAAGGTAATGTTGCACATTGGGATTGACACTGTGCAAATTAACGCCAAAAAACAACCGGGCGAACCACTAGAAGTGTTTGACATTAAGACCAAACAGGGTGAGTACACTAAGGAAAAGAGTGAAAGTGTAGTGGAAGTTGATTTAAAGAAACTCAGCAAGAAGTACAACCCAATTACCCCATTTGTGGTAATGAAGGAATCACTAGAAAACTTTAAATTAGTGCCAATCCGCCAACGCGGTGAGATTAAAGTGGGCCAACCAATCTTTAAACTAGTCTACAAGAAGTCACAGGCATAA", "aa": "MNILGANVGQTFSGSFIDFILYGALPDGRRWLANSYLVPIIGLFLAAIYFPTFYFLIKHFNLATPGRGGKLITKKEYLASKAAAKAEGVSGVAENFTQTQIEAGILLQAYGGKENIVELGACITKLRVTVKNPELVKEEPIKELGAAGVMRTTPTFFVAVFGTRAAVYKSAMQDIIQGKVNWEALQKVINTDQLAVEPKETTPPKEVMPVVQDEIVILSPVNGTLKSLNQVPDETFKQKLVGEGVAIVPSDGHFKAPGEAGVKTELAFPGGHAYIFDIDGIKVMLHIGIDTVQINAKKQPGEPLEVFDIKTKQGEYTKEKSESVVEVDLKKLSKKYNPITPFVVMKESLENFKLVPIRQRGEIKVGQPIFKLVYKKSQA", "aa_hexdigest": "a5a856f4cedb2962e2a773fd71348971", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsG" ], "truncated": "5-prime", "pseudogene": { "start": 250027, "stop": 251466, "observations": { "mutation": [ 250276 ], "stop": [ 250276 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75569", "query_cov": 1.0, "subject_cov": 0.4031914893617021, "identity": 1.0, "score": 731.0, "evalue": 1.55e-257, "gene_start": 1, "gene_end": 379, "reference_start": 562, "reference_end": 940, "reference_sequence": "MQIKAQDTGQQKKSCLLSNIRNKWKNRNRGSFRQWVGKLSNGLMIPIAVLPIAGIFLGVGDAIAGNAGDLTGLRYFGLFIKNGGDVVFANLPILFAIAIAITFSQDAGVAGFSAFVFWAAMNGFMSSLILPFDKAGKIITDTSTPIAGFKVLYNKSVPVHAIATTLGLRTLSTSVFGGIIVGALTSVLYKKFYAIRLPDVIGFFSGTRFVPIICFVVAIPVALILLMIWPAVSIGLNAIGTGLGFLGGKGYGANSLIFGYIERSLIPFGVHHAFYAPLWYTSAGGSLQEIVNQQVWIRPDFHLSDNYVARVIGWVDPNNSSMYIIPGALNGQNGSSTGNTMSKDLNGALSAYMSKESTAFLTWKDLVDGLTFKGNFDKMAENGLLDGSNKIWLGLNGSGILGKKLLLSDGNVYTITFKTFANTTPIAWSKGAQAVLPLNASSTIVNNPTALAAATQSNNNTNNIKLYPVNSFRVAVESLNPAQYSQGKFPFMLFGIPAAGVAMILAAPKDRRKEAASIVGSAAFTSFLTGITEPFEFTFLFLAPWLFYGVHAVLAAVSFWLMNILGANVGQTFSGSFIDFILYGALPDGRRWLANSYLVPIIGLFLAAIYFPTFYFLIKHFNLATPGRGGKLITKKEYLASKAAAKAEGVSGVAENFTQTQIEAGILLQAYGGKENIVELGACITKLRVTVKNPELVKEEPIKELGAAGVMRTTPTFFVAVFGTRAAVYKSAMQDIIQGKVNWEALQKVINTDQLAVEPKETTPPKEVMPVVQDEIVILSPVNGTLKSLNQVPDETFKQKLVGEGVAIVPSDGHFKAPGEAGVKTELAFPGGHAYIFDIDGIKVMLHIGIDTVQINAKKQPGEPLEVFDIKTKQGEYTKEKSESVVEVDLKKLSKKYNPITPFVVMKESLENFKLVPIRQRGEIKVGQPIFKLVYKKSQA" }, "paralog": false, "identity": 0.9979123173277662, "score": 938.0, "evalue": 0.0, "description": "Internal stop codon at 250276. Nonsense mutation around 250276.", "psc": { "uniref90_id": "UniRef90_P75569", "gene": "ptsG", "product": "PTS system glucose-specific EIICBA component", "ec_ids": [ "2.7.1.199" ], "uniref50_id": "UniRef50_P47315", "cog_id": "COG2190", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0016301", "GO:0008982", "GO:0090563", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75569", "EC:2.7.1.199", "UniRef:UniRef50_P47315", "COG:COG2190", "COG:G", "GO:0005886", "GO:0016301", "GO:0008982", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47315", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47315" ], "product": "PTS system glucose-specific EIICBA component" } }, "id": "CNOCLNGIAL_485", "locus": "GCA000143945_00450" }, { "type": "cds", "sequence": "contig_1", "start": 252076, "stop": 252411, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAACTGATTACAACACCTGTAGAAACCACCGCCAAAGCGGAACTCGTTTCCCTAGCTAAGCTCGGGGAAATGCGTACCCATGTCGGAATGGTTAAGCGTTACTGGAACCCCAAAATGGGCTTTTTTATTGAACCAGAACGCAAGCACAATAACGATCACTTTGTATTAGAGTTACAACGTCAATCTTTGCAAACAGCTTACAACTACGTCAAGGAAGTAGCACAAAACAACGGTCAAATTCTCTTCGTTGGTACTAAGAACGATTATGTGAAAAAACTCGTTAATAATATCGCTAAACGGGTTCATGTTGCTTTTATTACTCAGCGCTGA", "aa": "MSELITTPVETTAKAELVSLAKLGEMRTHVGMVKRYWNPKMGFFIEPERKHNNDHFVLELQRQSLQTAYNYVKEVAQNNGQILFVGTKNDYVKKLVNNIAKRVHVAFITQR", "aa_hexdigest": "ede2eb867c81c97584ea6d007c805a42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12861.739699999984, "isoelectric_point": 9.740608787536619 }, "id": "CNOCLNGIAL_486", "locus": "GCA000143945_00451" }, { "type": "cds", "sequence": "contig_1", "start": 252412, "stop": 252960, "strand": "+", "frame": 1, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_UPI00132F76CA", "UniRef:UniRef90_UPI00132F76CA" ], "nt": "TTGGGTGGTACTTTAACTAACTTTAAAACACTTTCAATTTCAATTAATAAACTCAATAAGTTAGTGGAAAAACAAGCGGAAAACGCGGCTGATTTAACTAAAAAGGAAAACTTGATGCTTTCCCGCGAAATTGAACGCTTGGAGAAGTTCTTTGGCGGGGTAAAATCACTTAAACGTTTACCGAACTTACTAATTGTGGATGACCCGGTATACGAAAAGAATGCGGTAGCGGAGGCTAACAGCTTGAGAATTCCTGTAGTAGCGTTGTGTAACACTAACACCAATCCGGAATTAGTTGACTTTATTATTCCCGCTAACAACCACCAACCCCAATCAACCTGCTTGTTGATGAACTTATTAGCTGATGCGGTGGCTGAAGCTAAGGCAATGCCAACCATGTTTGCTTACAAACCGGATGAGGAAATTCAAATTGAAATTCCCCAAAAACAAGAGGCACCACGCCAAGTTGTAAACCGTGCAAATTCTAAACAAATTACCTCTCAACGGTTAAACATTACTCGCAACCCAGAAGTTCTCACCCGTGAATAA", "aa": "MGGTLTNFKTLSISINKLNKLVEKQAENAADLTKKENLMLSREIERLEKFFGGVKSLKRLPNLLIVDDPVYEKNAVAEANSLRIPVVALCNTNTNPELVDFIIPANNHQPQSTCLLMNLLADAVAEAKAMPTMFAYKPDEEIQIEIPQKQEAPRQVVNRANSKQITSQRLNITRNPEVLTRE", "aa_hexdigest": "2d2a9480b5bba35f7e805dbaaced282e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00132F76CA", "query_cov": 1.0, "subject_cov": 0.9054726368159204, "identity": 0.995, "score": 344.0, "evalue": 3.18e-119, "valid": true, "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_UPI00132F76CA", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00132F76CA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132F76CA" ], "product": "30S ribosomal protein S2" }, "genes": [ "rpsB" ], "id": "CNOCLNGIAL_487", "locus": "GCA000143945_00452" }, { "type": "cds", "sequence": "contig_1", "start": 253214, "stop": 253768, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGCCAACTCCCTGATTGGCTCCATTCAGGAATTTAAAGCGCAAAAGTCAGCTCACACCTTAAAGTCCCTAACGCAACCCTTTACGCGGGTATTCCGTGAAGAAGGCTTGGTTTCGTTACCGGTGGGTGAAGTTGTTGTCGGTGACATTATCTTTCTGGAAGCGGGTGATATTATTCCCGCTGACGGGAAGGTGCTGCAAGCGAACCACCTCCGCTGTATGGAGAGCTTTTTAACTGGTGAATCAGTCCCAGTTGATAAAAGTGTGGTTAACACCGGTGGCAAGGGTTTGTTGGAACAAACAAACCTACTCTTTTCTGGTGCCCAAGTGGTCTTTGGTAGTGGTGTATTCGAAGTAACTGCTGTGGGGTTAAATACCCAAGTCGGTCAAATTGTCAAAACAGTGGACAGTAGTGCAACTAAGCTATCGCCCCTACAGCAAAAACTGGAAAAAGTGGGGAAGTGGTTTAGCTGGTTTGGCCTTGGTTTATTCGTTGTCGTTTTCCTAGTGCAGTTAGGGTTACTTGGTTTTCACAACTTTAGCGCTAACTGA", "aa": "MLANSLIGSIQEFKAQKSAHTLKSLTQPFTRVFREEGLVSLPVGEVVVGDIIFLEAGDIIPADGKVLQANHLRCMESFLTGESVPVDKSVVNTGGKGLLEQTNLLFSGAQVVFGSGVFEVTAVGLNTQVGQIVKTVDSSATKLSPLQQKLEKVGKWFSWFGLGLFVVVFLVQLGLLGFHNFSAN", "aa_hexdigest": "cde4ae56015048cc887e0fd91da2a783", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19722.599000000002, "isoelectric_point": 6.286415290832521 }, "id": "CNOCLNGIAL_488", "locus": "GCA000143945_00453" }, { "type": "cds", "sequence": "contig_1", "start": 253826, "stop": 254677, "strand": "+", "frame": 2, "gene": null, "product": "cation-transporting P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A405064", "UniRef:UniRef90_UPI000A405064" ], "nt": "GTGACCTTTATTAACGTCATTTTTGCCTTAAGTGTGCAGAAGCTCACCAAACAAAAGGCAATTATTAAGTACCTAGCTGCAATCGAAACACTCGGTGGCGTGCAAATCATTTGTACCGACAAAACTGGTACTTTAACACAAAACAAGATGAAGGTCGTGGACTATTTCTGTTTTAGTAACACTACCCAAACAGACTTAGCACGTGCTTTGTGTTTGTGCAACAATGCTACAGTCAACACTAACGGAGACAGCACCGGTGATCCAACTGAAATTGCCCTTTTGCAATGGTTGGACCGTGATGGTTTGGAATTAAACCACTACACACGCGTGTACGAAAAGGCATTTGATTCCAACCGCAAGCTTATGAGTGTGGTGGTGCAAAAGGACAACCGTTTTATTGTGATTGTCAAGGGCGCACACGATGTCTTGTTACCGTTATGTAAGGGCTTGGATAGCAACCAAATTAAGCCTTTAATTGATGAACGTGCTAGCAACGGCTTGCGCAACTTAGCGGTGGGACTCAAGGTTTTATACTGCTTTGATCCAGAAAATACCCAAACTGTCAACGAATTGGAAAGCGAACTCGACTTTTTGGGTTTTGTTAGCTTACAAGATCCACCACGAATTGAAAGTAAAGCTGCCATTATGGCGTGTCACCAAGCCAACATTACCCCAATTATGATCACTGGTGATCACCTCAAAACCGCTACTGCAATTGCCAAAGAATTGGGCATTCTGACCGATGAAAGGCAAGCCATTTTAGGAGTCGATTTAGACCCCGCTAAAATTATGGAGTACCGCGTCTTTGCCAGAGTAACGCCGCAGCAAAAGCTAGAAATAGTTAATGCCTGA", "aa": "MTFINVIFALSVQKLTKQKAIIKYLAAIETLGGVQIICTDKTGTLTQNKMKVVDYFCFSNTTQTDLARALCLCNNATVNTNGDSTGDPTEIALLQWLDRDGLELNHYTRVYEKAFDSNRKLMSVVVQKDNRFIVIVKGAHDVLLPLCKGLDSNQIKPLIDERASNGLRNLAVGLKVLYCFDPENTQTVNELESELDFLGFVSLQDPPRIESKAAIMACHQANITPIMITGDHLKTATAIAKELGILTDERQAILGVDLDPAKIMEYRVFARVTPQQKLEIVNA", "aa_hexdigest": "e5039c6d0d8a6fe02b4597dc799f2781", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 253526, "stop": 254588, "observations": { "insertion": [ 254513 ], "mutation": [ 253766 ], "stop": [ 253766, 254562 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A405064", "query_cov": 0.8056537102473498, "subject_cov": 0.41834862385321103, "identity": 1.0, "score": 450.0, "evalue": 2.47e-154, "gene_start": 2, "gene_end": 229, "reference_start": 293, "reference_end": 520, "reference_sequence": "MNKWTGLSAAAVLESRAQHGANLIPTKKLTPFWLLFLEQFKSLVVILLLVATILSLVVAIISGVNANWLFDHNLVIEWTQPFVILITVLANSLIGSIQEFKAQKSAHTLKSLTQPFTRVFREEGLVSLPVGEVVVGDIIFLEAGDIIPADGKVLQANHLRCMESFLTGESVPVDKSVVNTGGKGLLEQTNLLFSGAQVVFGSGVFEVTAVGLNTQVGQIVKTVDSSATKLSPLQQKLEKVGKWFSWFGLGLFVVVFLVQLGLLGFHNFSANWSIALIGAIALVVAIIPEGLVTFINVIFALSVQKLTKQKAIIKYLAAIETLGGVQIICTDKTGTLTQNKMKVVDYFCFSNTTQTDLARALCLCNNATVNTNGDSTGDPTEIALLQWLDRDGLELNHYTRVYEKAFDSNRKLMSVVVQKDNRFIVIVKGAHDVLLPLCKGLDSNQIKPLIDERASNGLRNLAVGLKVLYCFDPENTQTVNELESELDFLGFVSLQDPPRIESKAAIMACHQANITPIMITXVITSKPLLQLPKNWAFWPMKGKPF" }, "paralog": false, "identity": 0.9915254237288136, "score": 680.0, "evalue": 5.06e-247, "description": "Internal stop codon at 253766, 254562. Frameshift due to insertion around 254513. Nonsense mutation around 253766.", "psc": { "uniref90_id": "UniRef90_UPI000A405064", "product": "cation-transporting P-type ATPase", "uniref50_id": "UniRef50_UPI000A405064", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A405064", "UniRef:UniRef50_UPI000A405064" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A405064", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A405064" ], "product": "cation-transporting P-type ATPase" } }, "id": "CNOCLNGIAL_489", "locus": "GCA000143945_00454" }, { "type": "cds", "sequence": "contig_1", "start": 254762, "stop": 255157, "strand": "+", "frame": 2, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA", "UniRef:UniRef90_UPI001FB434DA" ], "nt": "ATGGGACAAACGGGGGTTGACATTGCCAAAGATGCCGCTGATGTCATTATTAGCGATGACAACTTTGCCACAATTGTTAACGGGATTGAACAGGGTCGTAAAACCTTTTTGACCTGTAAACGGGTGTTGTTTAACCTCTTTTTAACCTCAATTGCTGGCACGATTGTCGTGTTATTGGGTTTATTTGTATTAGGGGAAGTCTTTCGTGAACAACTCAGCAAAGCAAACCATAACTTCCAGGTCTTTACCCCTACGCAACTGCTAATTATTAACCTCTTTGTTCACGGCTTTCCCGCTGTGGCCTTAGCGATCCAACCCGTCCAGGAAAAACTGATGTTAAAACCGTTTTCCACCAAAAATCTGTTTTACAACCGTGGCGGGTTCGATTTAATCTGA", "aa": "MGQTGVDIAKDAADVIISDDNFATIVNGIEQGRKTFLTCKRVLFNLFLTSIAGTIVVLLGLFVLGEVFREQLSKANHNFQVFTPTQLLIINLFVHGFPAVALAIQPVQEKLMLKPFSTKNLFYNRGGFDLI", "aa_hexdigest": "dbc40f381a642a7fb508399d7de6d4db", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 254552, "stop": 255379, "observations": { "mutation": [ 254675, 255155 ], "stop": [ 254675, 255155 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FB434DA", "query_cov": 1.0, "subject_cov": 0.4729241877256318, "identity": 1.0, "score": 255.0, "evalue": 7.69e-84, "gene_start": 1, "gene_end": 131, "reference_start": 71, "reference_end": 201, "reference_sequence": "MGILTDERQAILGVDLDPAKIMEYRVFARVTPQQKLEIVNAWKQAGYTVAVTGDGVNDAPALVTSDVGCCMGQTGVDIAKDAADVIISDDNFATIVNGIEQGRKTFLTCKRVLFNLFLTSIAGTIVVLLGLFVLGEVFREQLSKANHNFQVFTPTQLLIINLFVHGFPAVALAIQPVQEKLMLKPFSTKNLFYNRGGFDLIWQSLLLSFLTLLFYSLGMVYAINDPELGKSGDLINRAGATCGFMVLGGSAALNSLNLMVDRPLVATNPKHYGIVXX" }, "paralog": false, "identity": 0.9890909090909091, "score": 531.0, "evalue": 4.2e-195, "description": "Internal stop codon at 254675, 255155. Nonsense mutation around 254675, 255155.", "psc": { "uniref90_id": "UniRef90_UPI001FB434DA", "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FB434DA", "UniRef:UniRef50_UPI001FB434DA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA" ], "product": "HAD-IC family P-type ATPase" } }, "id": "CNOCLNGIAL_490", "locus": "GCA000143945_00455" }, { "type": "cds", "sequence": "contig_1", "start": 255170, "stop": 255379, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCTTTTTAACCCTACTTTTCTACAGTTTAGGGATGGTCTATGCCATTAATGATCCGGAGTTAGGCAAATCCGGTGATTTAATTAACCGGGCTGGTGCGACCTGTGGTTTTATGGTGTTAGGGGGCAGTGCAGCTTTAAATTCCTTAAACCTAATGGTGGATCGACCATTGGTAGCTACTAACCCAAAGCACTATGGCATTGTGTGA", "aa": "MSFLTLLFYSLGMVYAINDPELGKSGDLINRAGATCGFMVLGGSAALNSLNLMVDRPLVATNPKHYGIV", "aa_hexdigest": "90d89e9adaf1a3081a1b760d3f328d08", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7320.5107000000025, "isoelectric_point": 6.508485221862792 }, "id": "CNOCLNGIAL_491", "locus": "GCA000143945_00456" }, { "type": "cds", "sequence": "contig_1", "start": 255389, "stop": 255571, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCCTCGATCTTTGTCTTCTTATTAATTATTTTCATTAACCCCTTGGGGTTAGTGTTTAGTACATTAAAAGACCTTACTGCCCACCCGGTTTTAATTGGTTATAGCTTTGGGGGCGTGCTTTTATATATGACAATTAACGAAGTTGTTAAACTTATTAGGTTAAGTTATGGGTCTGTTTAA", "aa": "MSSIFVFLLIIFINPLGLVFSTLKDLTAHPVLIGYSFGGVLLYMTINEVVKLIRLSYGSV", "aa_hexdigest": "38c61e2afc5f1f446e6fe3015195232c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6634.966199999999, "isoelectric_point": 8.160553169250488 }, "id": "CNOCLNGIAL_492", "locus": "GCA000143945_00457" }, { "type": "cds", "sequence": "contig_1", "start": 255558, "stop": 257720, "strand": "+", "frame": 3, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_P47318", "UniRef:UniRef90_P75559" ], "nt": "ATGGGTCTGTTTAACTTCTTGAAGTTAGTCTCCCCGCGTCATCGGATTTACCATAAGGCAAGTAAGATTGCCAACGAAGTCGAGGGACACAAAAATTACTACCGCAACCTCACTGATGTACAGCTGCTGGAAGAGTCCAATAAGCTAGTGGATTTAGTGACAAAGCAAAACTACACCATTTTGGATGTGGCGGTGGCTGCTTTGGCCTTAATTCGCGAAGTGGTCTACCGTGAAACCGGGGAGTTTGCTTACAGAGTGCAAATTATTGGTGCTTACATCGTCTTGATCGGTGACTTTGCTGAAATGATGACTGGGGAAGGGAAAACCCTGACCATTGTTTTAGCAGCCTATGTCTCGGCGTTAGAAAAACGCGGTGTCCATGTGGTTACGGTCAATGAATACCTAGCGCAGCGGGATGCTACTAACGCCACCAAGATCTTAAAGCGTGTTGGCATGACCGTTGGTTGTAACTTTGCTAACCTAGCACCACATTTAAAGCAAGCCGCGTTTGCTTGTGATGTAACTTACACCACTAACAGTGAACTCGGTTTTGATTATCTCAGGGACAATATGGTGCACCGCTTTGAGGACAAGAAGATCCGCGAGTTGCACTTTGCGATAGTAGACGAAGGCGATTCGGTTCTCATTGATGAAGCGCGCACCCCTTTAATTATTTCTGGTCCTGCCAAAAACGAGTTTGCAGCATACGTGGCTGTTGATCGCTTTGTCAAAAAGCTCAAGGAAGACGAGTACAAGATTGACCCCGAATCACGTGCCCCCGCTTTAACAGAACTTGGCATTAAGCACGCGGAAAAGAACTTTAAAACCGATAACCTGTTTGCGCTAGAAAACAGTGACCTGTTCCACAAGATTATTAACGCTTTAACCGCCGTTAAAGTTTTTGAGCAGGGCAAGGAGTACATTGTGCGTGATGGTAAGGTGTTAATTGTGGACCACTTTACTGGCCGAATCCTGGAGGGCAGGAGCTACAGTAATGGGCTCCACCAAGCGGTTCAGGCCAAGGAAATGGTCGAAATTGAACCAGAAAATGTGATAGTAGCTACGATTACCTACCAATCGTTTTTCCGTCTGTACAACCGGTTGTCAGCGGTATCGGGTACCGCTTTTACTGAATCGGAGGAATTCCTCAAGATTTACAACATGGTGGTAGTACCCGTACCTACTAACCGACCCAATATCCGCAAAGACCGTGCTGACAGCGTCTTTGGTACGCCCAACATTAAGTGGCTAGCAGTGGTCAAGGAAGTCAAACGGATCCACGAAACCGGGCGACCAATCCTAATTGGGACCGCCAACATTGATGACTCGGAACTACTTCACAACTACCTCCAAGAAGCCAACATTCCCCACGAAGTTCTCAACGCCAAGAACCACTCTCGGGAAGCGGAAATAGTCGCTAAGGCGGGCCAAAAAGGGGCGGTGACCATTTCTACCAATATGGCGGGAAGAGGTACTGACATTCGTTTAGGTGAAGGGGTCGCTGAAATGGGCGGGTTGTATGTCCTTGGTACGGAACGCAACGAATCGCGCCGGATTGACAACCAACTGCGCGGTCGGGCGGGTCGGCAAGGCGATCGCGGTGAGACCAAGTTCTTTATCTCCTTGGGTGATGCCCTGTTCAAACGCTTTGCCCATGACCGGATTGAACGCGCCATTACTAAGTTAGGGAACGATACCTTTGACAGCTCCTTCTTTTCCAAGATGTTAAGCCGTACCCAAAAACGGGTCGAAGCAATTAACTTTGACACCAGAAAGAACTTAATTGATTATGACCATGTGTTGGCCAGTCAACGGGAATTGATCTACAAACAACGCGATAAGTTCTTATTAGCCACTGATCTCAGTGACATGATCGACAAGATGTTGGAAAAGTTTGTCGAGCAGTTCTGTGACCAGTACCGCAACCCTAAGAACCAAAACCTCGTTAACCACATTGCTTTATCAGAGGCCTTAAACCTGGAATTAAACATGCACGGTGTTATTAGTCCGAAGCTGTTTGAAAACATGACTTTTGATGCGACTGTCCATAAAACCCACAGTTTAATTGGTGAAAAGATTACCAATAAGGTCAAGGTCTTAACCCCCCCAATTGCCCTCATCCGTTTTCGTGAAATTATGATCACAGCAATGGACAAGCACTGA", "aa": "MGLFNFLKLVSPRHRIYHKASKIANEVEGHKNYYRNLTDVQLLEESNKLVDLVTKQNYTILDVAVAALALIREVVYRETGEFAYRVQIIGAYIVLIGDFAEMMTGEGKTLTIVLAAYVSALEKRGVHVVTVNEYLAQRDATNATKILKRVGMTVGCNFANLAPHLKQAAFACDVTYTTNSELGFDYLRDNMVHRFEDKKIRELHFAIVDEGDSVLIDEARTPLIISGPAKNEFAAYVAVDRFVKKLKEDEYKIDPESRAPALTELGIKHAEKNFKTDNLFALENSDLFHKIINALTAVKVFEQGKEYIVRDGKVLIVDHFTGRILEGRSYSNGLHQAVQAKEMVEIEPENVIVATITYQSFFRLYNRLSAVSGTAFTESEEFLKIYNMVVVPVPTNRPNIRKDRADSVFGTPNIKWLAVVKEVKRIHETGRPILIGTANIDDSELLHNYLQEANIPHEVLNAKNHSREAEIVAKAGQKGAVTISTNMAGRGTDIRLGEGVAEMGGLYVLGTERNESRRIDNQLRGRAGRQGDRGETKFFISLGDALFKRFAHDRIERAITKLGNDTFDSSFFSKMLSRTQKRVEAINFDTRKNLIDYDHVLASQRELIYKQRDKFLLATDLSDMIDKMLEKFVEQFCDQYRNPKNQNLVNHIALSEALNLELNMHGVISPKLFENMTFDATVHKTHSLIGEKITNKVKVLTPPIALIRFREIMITAMDKH", "aa_hexdigest": "1ac3bdcbdafd767446dd7a8ddd0a26a8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75559", "query_cov": 1.0, "subject_cov": 0.8910891089108911, "identity": 1.0, "score": 1370.0, "evalue": 0.0, "valid": true, "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_P47318", "cog_id": "COG0653", "cog_category": "U", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_P47318", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47318" ], "product": "Protein translocase subunit SecA" }, "genes": [ "secA" ], "id": "CNOCLNGIAL_493", "locus": "GCA000143945_00458" }, { "type": "cds", "sequence": "contig_1", "start": 257775, "stop": 257984, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAACGAGTCCCCTCAACGTTTACATTCGCGAAACCGACATCTTGTTCCAAACAATGTTGCAAAAGATTGCTCGGGACGTCATTATCCAAATTGCTAACTTAGCGACTCCCGAAGAGTTTGATGAGGAACTAATGAAGGCTAACGCCTTGAAGAAGTTGCAAGCACTTAGAGAAGCTCATGAAAAGTCCAACCAAGGACAGTAA", "aa": "MEQTSPLNVYIRETDILFQTMLQKIARDVIIQIANLATPEEFDEELMKANALKKLQALREAHEKSNQGQ", "aa_hexdigest": "1117da0cfbdeb69604f76a8b26d378b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7957.036599999999, "isoelectric_point": 4.997818565368651 }, "id": "CNOCLNGIAL_494", "locus": "GCA000143945_00459" }, { "type": "cds", "sequence": "contig_1", "start": 257959, "stop": 259932, "strand": "+", "frame": 1, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887", "KEGG:K03702", "RefSeq:WP_010874568.1", "SO:0001217", "UniParc:UPI0000137EBA", "UniRef:UniRef100_P75558", "UniRef:UniRef50_P47319", "UniRef:UniRef90_P75558" ], "nt": "ATGAAAAGTCCAACCAAGGACAGTAAACTGTTCCACCTCAAGAGTAACTTTGCCCCCACTGGGGACCAACCAGCGGCGATTGCTAAGTTAGCTGAGTTTCAAACCAATGAACAGGTCTTACTCGGTGCTACGGGTACCGGTAAGACCTTTACGATTGCCAACGTTATTCAAAAGGTACAACTGCCTACAGTAGTGATTGCCCACAATAAAACGTTAGCGGGACAGCTTTATCAGGAGTTGAAGGAGTTATTCCCTAATAATGCGGTGGAGTACTTCATCTCCTACTTTGACTTTTACCAACCAGAGGCTTATCTGCCCGCTAAGGGGGTTTACATTGAAAAGAGTGCAACTGTTAACGAAGAGATTAAACGGTTACGAGTGTCCACCTTACATTCACTCTCTACCCGCAAGGATGTGATTGTGGTGGGTTCGGTAGCCAGTATTTACCCAACTTCTTCTCCCGCTGATTTCGCCCAGTACTCGCTCTGGTTAGTAGTTGGTAAGGAATACGGTTTAAGTGAGTTAAAAACGCAGTTAATTCACCTAAACTATGTTGTTAATAAACAACAGTTAACTCCGGGAAAGTTCCGCTTTCAGGGCGATGTTGTTGAAGTGTTTCCTGGTTATGCGCAGGATTATGTCTTGCGGCTTTCCTTCTTTGACCAGCAACTAGAACAAATTGCTCGCATCGATCCCTTAACTAACAAGGTATTGGAAACCCTTAACAGCTTTAAGTTAGGTCCCGCTGATGAGTACATAGTAAACCAAAATGACTTAGGAGTAGCCTTAGACACGATTAAGGCCGAACTCAAAGATCGCTTAAAGTACTTTGAACGGTTAAACTTCCCCGAACGTGCCCAGCGCTTACAAACGATTACCGAACACGATTTAGCAGATCTGAAGGCCTGGGGGGTATGTAGTGGCGTGGAAAACTATGCGCGGCATTTAGAACACCGCCCACCGCACTCCAAGCCGTATAACATCTTTGATTACTTTACTAAAGGTGAATGGCTATTGGTAGTTGATGAATCCCACCAAACGCTACCGCAAATTAAGGGGATGTACAACACCGATATTTCACGGAAACAAAGCCTAATTGAGTACGGTTTTCGCCTCCCTTCAGCTTTAGACAACCGTCCTTTATCCTATGAGGAATTCCGCCAGGGAATCAACAAGGTCATTTATGTATCCGCTACCCCTAGGGAAGAGGAAATTCAGTTAAGTCACAACAACGTGGTCGAACAGTTAGTGCGTCCCACCTACCTCTTGGACCCGGAAGTGATTGTGAAACCGAAGGACAACCAGGTTGAAGACCTAGTGAGTGAAATCATTAACCAAAGAAAACACAACGGTCGTACCTTTGTCACGGTGTTAACGATTAAGATGGCGGAAAACCTGACTGACTTTTTGAAGGAGCGCAACATTAAGGTAGCGTACATCCACAAGGATATTAAAGCCTTGGAACGTTTAATTCTGTTAACGGATTTACGCAAAGGTGAGTATGAGTGTTTAGTGGGGATTAACTTACTGCGAGAAGGTTTAGATGTTCCTGAAGTTTCACTCGTAGCCATCTTTGACGCTGACATTCCTGGATTACCACGCGATGAGCGCAGTTTAATCCAGATAATTGGCCGCGCTGCGCGAAACGTTCATGGCCGGGTGATTATGTATGCCAATACCATTAGCGAACAAATGGATAAGGCGATTAAGGAAACGCAACGCCGGCGCACCATCCAAATGGCTTACAACGAACAACACCACAAAACACCAATGACGGTGCAAAAACCAATTACCTTAAACCAGCCGATTAAGTTAAAGACCAAAAGCTCAGAGCAGCAAAAAGCTGCTTTAATTAAGCAGCTAACCAAGGAAATGAAGCAAGCAGCCGCTAACCAAAACTATGAGTTAGCGATTGAGATCCGCGACTCGATCTTTGAATTGGAAAAGCAATTTCGTGGTAAAATTAAGAGCTAG", "aa": "MKSPTKDSKLFHLKSNFAPTGDQPAAIAKLAEFQTNEQVLLGATGTGKTFTIANVIQKVQLPTVVIAHNKTLAGQLYQELKELFPNNAVEYFISYFDFYQPEAYLPAKGVYIEKSATVNEEIKRLRVSTLHSLSTRKDVIVVGSVASIYPTSSPADFAQYSLWLVVGKEYGLSELKTQLIHLNYVVNKQQLTPGKFRFQGDVVEVFPGYAQDYVLRLSFFDQQLEQIARIDPLTNKVLETLNSFKLGPADEYIVNQNDLGVALDTIKAELKDRLKYFERLNFPERAQRLQTITEHDLADLKAWGVCSGVENYARHLEHRPPHSKPYNIFDYFTKGEWLLVVDESHQTLPQIKGMYNTDISRKQSLIEYGFRLPSALDNRPLSYEEFRQGINKVIYVSATPREEEIQLSHNNVVEQLVRPTYLLDPEVIVKPKDNQVEDLVSEIINQRKHNGRTFVTVLTIKMAENLTDFLKERNIKVAYIHKDIKALERLILLTDLRKGEYECLVGINLLREGLDVPEVSLVAIFDADIPGLPRDERSLIQIIGRAARNVHGRVIMYANTISEQMDKAIKETQRRRTIQMAYNEQHHKTPMTVQKPITLNQPIKLKTKSSEQQKAALIKQLTKEMKQAAANQNYELAIEIRDSIFELEKQFRGKIKS", "aa_hexdigest": "75e1c30c7f4ab8519fd501ad689d7074", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137EBA", "ncbi_nrp_id": "WP_010874568.1", "uniref100_id": "UniRef100_P75558", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137EBA", "RefSeq:WP_010874568.1", "UniRef:UniRef100_P75558" ] }, "ips": { "uniref100_id": "UniRef100_P75558", "uniref90_id": "UniRef90_P75558" }, "psc": { "uniref90_id": "UniRef90_P75558", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "uniref50_id": "UniRef50_P47319", "cog_id": "COG0556", "cog_category": "L", "kegg_orthology_id": "K03702", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47319", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47319" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "CNOCLNGIAL_495", "locus": "GCA000143945_00460" }, { "type": "cds", "sequence": "contig_1", "start": 259935, "stop": 260342, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPNE_0244", "db_xrefs": [ "RefSeq:WP_010874569.1", "SO:0001217", "UniParc:UPI00001391FC", "UniRef:UniRef100_P0CJ80", "UniRef:UniRef50_P0CJ80", "UniRef:UniRef90_P0CJ80" ], "nt": "ATGCGTAAACTAATTAAATTAAACGTCATTGTCTTTGTCTTGTTGTACTTGGGCGAGCTGTTTGCCAGCCTTTCGTTCAAGTTAATCAGTTGCCTCAAGACACGCAACCAGTACTCCTTAAACGGGTACTATGCGTTGTTTGTCTTTGTCAACATCATCCAAAAGATGGCTAACTCTTTCCAAAAGTTAGCTTCCTCAGTTGTGTTGTTTGAAACTGAAATTAACGAATTTTTAGTTCTCTTTACTGATACAAAGAATAAGCGTGAGGAGAGTGAACCAGTGCGCCAGGTGTCAACAACCCAAGAGTATCACCAGGTTACGCTCGACCAACAACACTACTTTAACCACAAACTGAGCGATTACTTCCGTTTGTTTAAGGACAAAACTTTCTTCTTTGAAATTATCTAG", "aa": "MRKLIKLNVIVFVLLYLGELFASLSFKLISCLKTRNQYSLNGYYALFVFVNIIQKMANSFQKLASSVVLFETEINEFLVLFTDTKNKREESEPVRQVSTTQEYHQVTLDQQHYFNHKLSDYFRLFKDKTFFFEII", "aa_hexdigest": "7839f1432cde41d0c75f09a4d6153c07", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001391FC", "ncbi_nrp_id": "WP_010874569.1", "uniref100_id": "UniRef100_P0CJ80", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391FC", "RefSeq:WP_010874569.1", "UniRef:UniRef100_P0CJ80" ] }, "ips": { "uniref100_id": "UniRef100_P0CJ80", "uniref90_id": "UniRef90_P0CJ80" }, "psc": { "uniref90_id": "UniRef90_P0CJ80", "product": "Uncharacterized protein MPNE_0244", "uniref50_id": "UniRef50_P0CJ80" }, "pscc": { "uniref50_id": "UniRef50_P0CJ80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0CJ80" ], "product": "Uncharacterized protein MG074 homolog" }, "genes": [], "id": "CNOCLNGIAL_496", "locus": "GCA000143945_00461" }, { "type": "cds", "sequence": "contig_1", "start": 260447, "stop": 263335, "strand": "+", "frame": 2, "gene": null, "product": "putative MG075-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47321", "UniRef:UniRef90_P75556" ], "nt": "ATGAAGCTTAGTGCTATTATCTCCCTATCAGTCGCTGGTACTGTGGGAACAACTGCGGTGGTAGTACCTACAACTATAACGCTTGTAAATAAGACCCACCAAGTAGAACATGAATCAGAACAATCGGATTTTCAAGATATTCGCTTTGGTCTTAATAGTGTTAAGTTGCCAAAAGCACAGCCAGCTGCGGCAACTAGAATTACCGTGGAAAACGGGACTGATAAATTAGTCAACTATAAGTCCTCACCACAACAACTCTTTTTAGCGAAGAACGCGCTTAAGGATAAACTCCAAGGTGAGTTTGATAAATTCCTAAGTGATGCGAAGGCCTTCCCAGCGCTAACCGCTGATTTACAGGAATGGGTTGACCAACAGCTGTTTAATCCAAACCAAAGTTTCTTTGATTTAAGTGCGCCCAGGTCAAACTTTACCCTTTCATCTGACAAAAAGGCTAGTTTAGACTTTATCTTCCGCTTTACTAACTTCACCGAATCCGTTCAGTTGTTAAAACTACCAGAAGGTGTATCGGTTGTAGTTGACTCCAAACAAAGCTTTGATTACTATGTCAATGCTAGTGCCCAAAAATTATTAGTTCTACCGCTGTCTTTACCAGATTACACTTTGGGTTTAAACTATATGTTTGACCACATTACTTTAAACGGTAAGGTTGTCAATAAATTTAGTTTTAATCCGTTCAAAACGAATTTAAACCTCGCCTTTAGCAACGTTTACAATGGCGTTGATGTGTTTGAAGCACAAAAGAATTTAGTAGGTAAGGGTAAATACCTCAACACCCACGTGAAGGCTGAAGACGTAAAGAAGGACGTTAATGCCAACATTAAAAACCAATTTGACATTGCCAAAATTATCGCTGAGCTAATGGGTAAAGCCCTTAAAGAATTTGGCAATCAACAAGAAGGTCAACCATTATCCTTCCTAAAGGTAATGGATAAAGTTAAAGAAGATTTTGAAAAACTGTTTAACTTAGTCCGTCCTGGATTGGGTAAATTTGTTAAGGACTTAATCCAAAGTAGTAGTCAAGCAGAAAACAAGATAACTGTCTACAAGTTAATCTTTGACAACAAAAAGACCATCTTAAACCTACTTAAAGAGCTTTCCATTCCGGAATTAAACTCTTCTTTAGGTTTAGTGGACGTCTTGTTTGATGTCATTACTGACTCTGATGGTCTCTATGAAAGGTTGCAATCTTTCAAAGACTTAATCGTTCCAGCAGTTAAAACGAATGAAAAAACCGCGGCTTTAAGTCCATTAATTGAAGAGTTATTAACCCAAAAGGATACCTATGTGTTTGACTTAATTCAAAAACACAAGGGTATCTTGACTAACTTGTTAAAGAACTTCTTAGCTGATTTCCAAAAATCAACGCCGTTTATGGCTGATCAAGTAGCCATCTTCACTGAGTTATTTGACAACGAAGGTGCGTTTGATTTATTTGGTGAGGCTGACTTTGTTGACAAGATTGCCGAACTCTTCTTAACAAAGCGTACTGTTAAAAATGGTGAAAAAATTGAAACTAAAGATTCCCTACTGGTAACATCATTAAAGAGTCTTTTAGGGGAAAAGGTAGCTGCCTTAGATGATTTGTTAGATAGCTACATCTTTAAAAATGAATTACTTAACCGCAGTGTAGAAGTGGCTAAGGCTGAAGCTAAGGACACTAAAGGTGCTACCGATTACAAAAAGGAACAAGCTAAGGCGCTCAAAAAACTCTTTAAACACATTGGTGAAAACACTTTAAGTAAAACCAATCTCGATAAAATCACCTTAAAAGAAGTTAAAAACACCGAAAATGTTGAATTAGAAGAAACTGAAACAACCTTAAAGGTTAAAAAACTCGATGTTGAATACAAGGTAGAACTTGGCAACTTTGAAATCAAGAATGGCTTGATTAAGGCAATGCTCGAATTCTTGCCAGACCCTAAAGATTTAGAAACAACTTTAGATAAACTCTTGTTCAAAGGGGAAAGCTACAAAGCGATGAAAGACAAGTACATCAAGGAAGGTTTCCCTGGTTATGGTTGGGCTAAAGGAGTAGTCCCTGGGGCCTTTGAATCTATTGAAAATACTTTTAAGAGTGCTATTGATAAAACCAAGTCCATTCGTGATCTCTTTGGCGACATGCTCTTTGGTAACGATTTAAGCAGCGTTAAAGAAACTGATTCATTCATCACCCTAGGTGGTTCGTTCGACATTAAGTATGGCGGTGAAAATCTCAATGTGTTGCCAGCTTACTACTCTTTAATTAACAGCGAAATTGGCTATCAAATTATTGGTGTAGATACCACAATTGATGTAACTAAGGTAAAAGTTGAATTAAAAAACAAAGAGTATAAGGGTAAATCGCCAGCTATTAACGGTCAGGTGAAGTTGTCACAATCATTCTTTAATGTTTGGACAAATATGTTTGACAGCATTACCAAACAAATCTTCCAAAAGAAATACGAGTTCAAAGATAACATCCAAGTGTTTGCGCGAAACGAAGATAACACATCACGTTTAGAACTTGACATTTCTGATCCTGAACAACGGGTAATTCCATTTGCTTTTGTTGATGGCTTTGGCATTCAACTCAAAGCAGTTGACAAAAACATTACGAAAGAAGCAGGTAATACTGAGCCAAAATCTCCTGTAATTCAACTTTATGAGGCACTTAATAAAGAAAAAGATCAAAAACAACAAAGTAAACAATCTCCAAAACAACTTGATACTAAAACACAGTTGGGTTACCTATTGAAATTAGGCGACAATTGGAGTAAAGATGATTACAAAAGCTTAATTGATGATACGATCATCAATAACAACTATTTAGAGGCCAGCTTTAATTCCAAGATAACGGTTGATCGCTTGGGTATTCCTATTGACCTTTGA", "aa": "MKLSAIISLSVAGTVGTTAVVVPTTITLVNKTHQVEHESEQSDFQDIRFGLNSVKLPKAQPAAATRITVENGTDKLVNYKSSPQQLFLAKNALKDKLQGEFDKFLSDAKAFPALTADLQEWVDQQLFNPNQSFFDLSAPRSNFTLSSDKKASLDFIFRFTNFTESVQLLKLPEGVSVVVDSKQSFDYYVNASAQKLLVLPLSLPDYTLGLNYMFDHITLNGKVVNKFSFNPFKTNLNLAFSNVYNGVDVFEAQKNLVGKGKYLNTHVKAEDVKKDVNANIKNQFDIAKIIAELMGKALKEFGNQQEGQPLSFLKVMDKVKEDFEKLFNLVRPGLGKFVKDLIQSSSQAENKITVYKLIFDNKKTILNLLKELSIPELNSSLGLVDVLFDVITDSDGLYERLQSFKDLIVPAVKTNEKTAALSPLIEELLTQKDTYVFDLIQKHKGILTNLLKNFLADFQKSTPFMADQVAIFTELFDNEGAFDLFGEADFVDKIAELFLTKRTVKNGEKIETKDSLLVTSLKSLLGEKVAALDDLLDSYIFKNELLNRSVEVAKAEAKDTKGATDYKKEQAKALKKLFKHIGENTLSKTNLDKITLKEVKNTENVELEETETTLKVKKLDVEYKVELGNFEIKNGLIKAMLEFLPDPKDLETTLDKLLFKGESYKAMKDKYIKEGFPGYGWAKGVVPGAFESIENTFKSAIDKTKSIRDLFGDMLFGNDLSSVKETDSFITLGGSFDIKYGGENLNVLPAYYSLINSEIGYQIIGVDTTIDVTKVKVELKNKEYKGKSPAINGQVKLSQSFFNVWTNMFDSITKQIFQKKYEFKDNIQVFARNEDNTSRLELDISDPEQRVIPFAFVDGFGIQLKAVDKNITKEAGNTEPKSPVIQLYEALNKEKDQKQQSKQSPKQLDTKTQLGYLLKLGDNWSKDDYKSLIDDTIINNNYLEASFNSKITVDRLGIPIDL", "aa_hexdigest": "c23c687391e0fcbf7d20ad606822323e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75556", "query_cov": 1.0, "subject_cov": 0.9339805825242719, "identity": 0.996, "score": 1824.0, "evalue": 0.0, "valid": true, "product": "Uncharacterized protein MG075 homolog", "uniref50_id": "UniRef50_P47321", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47321" ], "product": "Uncharacterized protein MG075" }, "genes": [], "id": "CNOCLNGIAL_497", "locus": "GCA000143945_00462" }, { "type": "cds", "sequence": "contig_1", "start": 263375, "stop": 263539, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGGTTCCTTACAACTTTACAGTAGTAGTGTTATCTTCCCATACGGTATTTATGACACCAGTGTTCAAGATGCTACGAAGATTGTGAAGCGTCTGAACTTTACTGACATGGGCTTCAAACTCAACGATCCAAAACCTAACTTCTGGTTCGTTGGTTTTTAG", "aa": "MQGSLQLYSSSVIFPYGIYDTSVQDATKIVKRLNFTDMGFKLNDPKPNFWFVGF", "aa_hexdigest": "b2df7e44a22662d28d9049daa275eeab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6213.053599999999, "isoelectric_point": 8.06275463104248 }, "id": "CNOCLNGIAL_498", "locus": "GCA000143945_00463" }, { "type": "cds", "sequence": "contig_1", "start": 263536, "stop": 263769, "strand": "-", "frame": 3, "gene": null, "product": "MPN214 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47322", "UniRef:UniRef90_P75555" ], "nt": "GTGTGTCTCCAAGGTATAGTGCGCTTAAAGACCTGTGATGGAGCACGGTACGAAATTAGCAAGTTTAACCAATATCTAAAACTAGGTAGTATATACGCCAAGCCCAACATTAGTTTTGACGAGTATAAAGCAAAAAGTTCATCCTACCGTAAACAAACCCGCGGGTTTTGGTGGATGAACTTCTCGCTTTACCTTCTAGGTAGCTTAATCAGTATAGTAGTTAGTCTGCTCTAA", "aa": "MCLQGIVRLKTCDGARYEISKFNQYLKLGSIYAKPNISFDEYKAKSSSYRKQTRGFWWMNFSLYLLGSLISIVVSLL", "aa_hexdigest": "5d740eb08b7127b983a78fc504f4c845", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 263536, "stop": 264000, "observations": { "mutation": [ 263778 ], "stop": [ 263778 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75555", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.987, "score": 154.0, "evalue": 1.62e-46, "gene_start": 1, "gene_end": 77, "reference_start": 78, "reference_end": 154, "reference_sequence": "MLVVQNILQKNDTSKYMLGTQTTNSKPREYGGLIVSTIYIVLFFAILNLTVFFNKTNNINLILKNSCVVSFVVVWLLVCLQGIVRLKTCDGARYEISKFNQYLKLGSIYAKPNISFDEYKAKSSSYRKQTRGFWWMNFSLYLLGSLISIVVSLL" }, "paralog": false, "identity": 0.987012987012987, "score": 300.0, "evalue": 1.03e-106, "description": "Internal stop codon at 263778. Nonsense mutation around 263778.", "psc": { "uniref90_id": "UniRef90_P75555", "product": "MPN214 family protein", "uniref50_id": "UniRef50_P47322", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75555", "UniRef:UniRef50_P47322", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47322" ], "product": "Uncharacterized protein MG076" } }, "id": "CNOCLNGIAL_499", "locus": "GCA000143945_00464" }, { "type": "cds", "sequence": "contig_1", "start": 263776, "stop": 263994, "strand": "-", "frame": 3, "gene": null, "product": "MPN214 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47322", "UniRef:UniRef90_P75555" ], "nt": "GTGGTTCAAAACATTCTACAAAAAAACGATACTAGTAAGTACATGTTAGGTACACAAACCACAAATTCGAAGCCGCGTGAATACGGCGGGTTAATAGTAAGCACAATTTACATTGTGCTTTTCTTCGCCATTTTGAATTTAACCGTTTTTTTCAATAAAACTAACAACATTAACCTAATTCTGAAAAACAGTTGTGTTGTTAGTTTTGTCGTTGTTTGA", "aa": "MVQNILQKNDTSKYMLGTQTTNSKPREYGGLIVSTIYIVLFFAILNLTVFFNKTNNINLILKNSCVVSFVVV", "aa_hexdigest": "35dc1714782f8651273b97459afd46a7", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 263536, "stop": 264000, "observations": { "mutation": [ 263778 ], "stop": [ 263778 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75555", "query_cov": 1.0, "subject_cov": 0.4675324675324675, "identity": 0.986, "score": 134.0, "evalue": 7.54e-39, "gene_start": 1, "gene_end": 72, "reference_start": 3, "reference_end": 74, "reference_sequence": "MLVVQNILQKNDTSKYMLGTQTTNSKPREYGGLIVSTIYIVLFFAILNLTVFFNKTNNINLILKNSCVVSFVVVWLLVCLQGIVRLKTCDGARYEISKFNQYLKLGSIYAKPNISFDEYKAKSSSYRKQTRGFWWMNFSLYLLGSLISIVVSLL" }, "paralog": false, "identity": 0.987012987012987, "score": 300.0, "evalue": 8.54e-107, "description": "Internal stop codon at 263778. Nonsense mutation around 263778.", "psc": { "uniref90_id": "UniRef90_P75555", "product": "MPN214 family protein", "uniref50_id": "UniRef50_P47322", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75555", "UniRef:UniRef50_P47322", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47322" ], "product": "Uncharacterized protein MG076" } }, "id": "CNOCLNGIAL_500", "locus": "GCA000143945_00465" }, { "type": "cds", "sequence": "contig_1", "start": 264183, "stop": 264383, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCAAGTATATTATTAAACGTTTGGGTTTGGCAGTGTTAGCGATGTTTATCGTGATGACCATTGTCTTCTTTTTGGTTAACTCCACAGGTCAAACGCCCTTATCAGCAACCTCTTCTAAGGATTTGGAGGCTGTCAAAACACAACTAGATGCCTTTGGTTTTAACGATCCGTTAATTGTGCGATATGGTCGTTACTGA", "aa": "MFKYIIKRLGLAVLAMFIVMTIVFFLVNSTGQTPLSATSSKDLEAVKTQLDAFGFNDPLIVRYGRY", "aa_hexdigest": "3809ea6829e43cd6717152c73b74461a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7421.7404000000015, "isoelectric_point": 9.599229621887208 }, "id": "CNOCLNGIAL_501", "locus": "GCA000143945_00466" }, { "type": "cds", "sequence": "contig_1", "start": 264405, "stop": 265145, "strand": "+", "frame": 3, "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "db_xrefs": [ "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75554", "UniRef:UniRef90_P75554" ], "nt": "TTGGGTACTTATTACAGCTCCCCGAACCAAACAATTGACCAAATTGTCTTTGGTCGGGTACCTAACACCCTTTACGTTGTTTTAATTTCTTTCTTTATCGGCTCTTTGTTGGGCATTATCTTCGGGATGATTTCCGGATTATTCCGTGGCAAGTTAATTGATGCGGTAATTAACGTGCTCGTTGTTTTATTTGTTTCGATTCCTTCCTTTGTGGTTGGTTTAGGTTTACTTAAGGCAGCTGGTTTGTTCCGTTTACCACCGCGCTTTATTAACTTCGATGATGCTAACTTTAACTTTGGTAACTTCTTGTTAGCCTCCATTATTCCTATTTTATCCTTGGTATTTTACACCTCAGCGGCCTTTACTTATCGGGTACGGAATGAGGTCGTGGAAGTAATGAACCAGGACTACATAAAAACAGCACGTAGTAAAGGTTTGAGTACTTTTGCTGTAGCGCTTTACCACATCTTCCGTAACTCAATTATTCCTTCTGTACCTTTATTTGTCTTTGGGATCTCTGGTGCCTTTTCGGGTGGTTTTATTATTGAATCCTTATTTGGTGTTCAGGGGGTATCACGGATCTTAATTGACTCGGTGCAAAGTAACGAAACTAACCTCGTGATGTTTAACATTATGTTCATCCAGGGGATTCCCTTACTAGCAAGTGTCTTCATTGAGTTGATTTATGTCCTAGTGGATCCTAGGATTCGGATTGCTAGTGCCGGTGGGGTGAGTCTGTGA", "aa": "MGTYYSSPNQTIDQIVFGRVPNTLYVVLISFFIGSLLGIIFGMISGLFRGKLIDAVINVLVVLFVSIPSFVVGLGLLKAAGLFRLPPRFINFDDANFNFGNFLLASIIPILSLVFYTSAAFTYRVRNEVVEVMNQDYIKTARSKGLSTFAVALYHIFRNSIIPSVPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQSNETNLVMFNIMFIQGIPLLASVFIELIYVLVDPRIRIASAGGVSL", "aa_hexdigest": "927a60e96aff7d810b1bbbb0bc289e61", "start_type": "TTG", "rbs_motif": null, "genes": [ "oppB" ], "truncated": "both", "pseudogene": { "start": 264183, "stop": 265397, "observations": { "mutation": [ 264381, 265143, 265179, 265191 ], "stop": [ 264381, 265143, 265179, 265191 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75554", "query_cov": 1.0, "subject_cov": 0.6089108910891089, "identity": 0.996, "score": 429.0, "evalue": 8.08e-149, "gene_start": 1, "gene_end": 246, "reference_start": 75, "reference_end": 320, "reference_sequence": "MFKYIIKRLGLAVLAMFIVMTIVFFLVNSTGQTPLSATSSKDLEAVKTQLDAFGFNDPLIVRYGRYWQTLFSGSLGTYYSSPNQTIDQIVFGRVPNTLYVVLISFFIGSLLGIIFGMISGLFRGKLIDAVINVLVVLFVSIPSFVVGLGLLKAAGLFRLPPRFINFDDANFNFGNFLLASIIPILSLVFYTSAAFTYRVRNEVVEVMNQDYIKTARSKGLSTFAVALYHIFRNSIIPSVPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQSNETNLVMFNIMFIQGIPLLASVFIELIYVLVDPRIRIASAGGVSLWTKLKFVYLRQAWLRKWRRINHTNSHNVLFNSPQHRQLLELKAIDYKHNTISLTEQQKTTLKIEPTANFVLLGTKCLKIITIHG" }, "paralog": false, "identity": 0.9876237623762376, "score": 732.0, "evalue": 1.35e-270, "description": "Internal stop codon at 264381, 265143, 265179, 265191. Nonsense mutation around 264381, 265143, 265179, 265191.", "psc": { "uniref90_id": "UniRef90_P75554", "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "uniref50_id": "UniRef50_P75554", "cog_id": "COG0601", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75554", "UniRef:UniRef50_P75554", "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75554", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75554" ], "product": "Oligopeptide transport system permease protein OppB" } }, "id": "CNOCLNGIAL_502", "locus": "GCA000143945_00467" }, { "type": "cds", "sequence": "contig_1", "start": 265224, "stop": 265397, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTTCAATTCGCCCCAACACCGCCAGCTGTTAGAGCTCAAAGCAATTGATTACAAGCACAATACCATTAGCTTAACTGAACAGCAAAAAACCACCCTTAAAATTGAACCAACTGCTAACTTTGTTTTGTTAGGAACTAAATGTTTAAAGATTATTACGATCCATGGATAA", "aa": "MLFNSPQHRQLLELKAIDYKHNTISLTEQQKTTLKIEPTANFVLLGTKCLKIITIHG", "aa_hexdigest": "19b9a9f4a652cc1778d49be5d039f633", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6532.632099999999, "isoelectric_point": 9.360825538635254 }, "id": "CNOCLNGIAL_503", "locus": "GCA000143945_00468" }, { "type": "cds", "sequence": "contig_1", "start": 265390, "stop": 265527, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAGCACCAAAAATTTGACCAAAGCCTGTTTCAACGGGTAGACATTAATGTCTTAAAACGCTCTGACCAGTTGATTGGCAAACCAACCACTAACTTTGTCGAAATTATGAAGCGGTTGTTCCAAAATAAGTGA", "aa": "MDKHQKFDQSLFQRVDINVLKRSDQLIGKPTTNFVEIMKRLFQNK", "aa_hexdigest": "894d7b30ce7129b6cb20d3a36f168ca6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5436.275199999999, "isoelectric_point": 9.991906547546389 }, "id": "CNOCLNGIAL_504", "locus": "GCA000143945_00469" }, { "type": "cds", "sequence": "contig_1", "start": 265552, "stop": 265875, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAATTATTTTGTTAGCCATCATTGTACCCTTAGCATCACCTTATTCAGCCGTTACTCCAGTATCGAACAACGCTTTGGCACAGAACCTACCACCACGATACCTCTGGAACGGCGCGGGTGACATTCAAGTAGAGAAAATTACGGCACGCTCGATTGCTGAAGTGGCCCAATCAAGTGGTGTTTTGGTAGGGAAATTACCGGAGGCAAGTAGCAATCCACTCGCTACTAACGTTAAGTACAACATTGCGCCTTACCAACTAGCAGAGCTGAAAAACTACTATCCATTATTAGGCACTAACGGTTTAGGGGTTGATATTTGA", "aa": "MLIILLAIIVPLASPYSAVTPVSNNALAQNLPPRYLWNGAGDIQVEKITARSIAEVAQSSGVLVGKLPEASSNPLATNVKYNIAPYQLAELKNYYPLLGTNGLGVDI", "aa_hexdigest": "8b299a0697ca8830e9c05c81a8acf52f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11331.995999999988, "isoelectric_point": 6.0894681930541985 }, "id": "CNOCLNGIAL_505", "locus": "GCA000143945_00470" }, { "type": "cds", "sequence": "contig_1", "start": 265921, "stop": 266148, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTAGCCTTAGTTTCAATGGTCTTCGGCACCATTTACGGCGCGATTGCTGGGAGCTTTGTTGGACGCGCTGCTGACAACATTATGAGCCGGATCATTGAAATTATTGATTTGGTTCCTTCAATTCTCTGGATCATTGTCTTGGGAGCAACATTCCGCTTTGGTGGGGTAAAACAATTTGATGACAGTGTGGTGATCTTTACCTTAATCTTTGTCTTTTGGACCTGA", "aa": "MVALVSMVFGTIYGAIAGSFVGRAADNIMSRIIEIIDLVPSILWIIVLGATFRFGGVKQFDDSVVIFTLIFVFWT", "aa_hexdigest": "47561f5ccf9a58cd93b6b075eb5b32ac", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8230.744499999997, "isoelectric_point": 4.777965354919433 }, "id": "CNOCLNGIAL_506", "locus": "GCA000143945_00471" }, { "type": "cds", "sequence": "contig_1", "start": 266254, "stop": 266520, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACCAGTTGTAACTGGTCGTTTAGCAGTCGTCTTTGTGAGTTTGATTCCCGCTGTGATTGGTTATGAAGCATCACTGGTTTTCTTAGGGCTTAAACCTGCTACCGAAGTGGGACTTGGTGCTTTATTAAACCAGGTCACCAGTAGTGGTAACATAGCACTGATTACCAGCTCAATTGTGAGCTTTGCAATTCTCACCGTTTCGACCCGCGTGTTTGCTAACGCTTTAAATGACGCAATTGACCCACGGGTAATAAGGAGGTAA", "aa": "MLPVVTGRLAVVFVSLIPAVIGYEASLVFLGLKPATEVGLGALLNQVTSSGNIALITSSIVSFAILTVSTRVFANALNDAIDPRVIRR", "aa_hexdigest": "5315e020ae5c14c46bf1431cf9d7c664", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9179.767299999989, "isoelectric_point": 9.961541938781735 }, "id": "CNOCLNGIAL_507", "locus": "GCA000143945_00472" }, { "type": "cds", "sequence": "contig_1", "start": 266522, "stop": 266821, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGCACTAAAAGCAACTAATTTTTTTACAGAACCAGAGTACAAACTGCAGGATGATTTGATCTTAGACATCCGTGATCTTCACGTGAGTTTTAAGGTTAAAGACGGCATTATGCAAGCTGTCCGTGGTGTTGATCTCAAAGTCAAAAAGGGCGCCATTGTCGGCATTGTGGGCGAATCTGGTAGTGGTAAATCGGTCTGTGTTAAGTCGATTATTGGCTTTAACGATGGCGCAAAAACGACCGCTAAGTTAATGAACTTTAAGAACATTGACATTAGCAAGATTAAAAAGCACCAGTGA", "aa": "MALKATNFFTEPEYKLQDDLILDIRDLHVSFKVKDGIMQAVRGVDLKVKKGAIVGIVGESGSGKSVCVKSIIGFNDGAKTTAKLMNFKNIDISKIKKHQ", "aa_hexdigest": "89a6349195c0e7dc09fdebe82169a6d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 42, "aa_cov": 0.42424242424242425, "hmm_cov": 1.0, "evalue": 2.2226477529446994e-09, "score": 33.50126647949219, "start": 42, "stop": 83 } ], "seq_stats": { "molecular_weight": 10851.64399999999, "isoelectric_point": 9.553457069396973 }, "id": "CNOCLNGIAL_508", "locus": "GCA000143945_00473" }, { "type": "cds", "sequence": "contig_1", "start": 266843, "stop": 268552, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGTCTTACATTTCCCAAGATCCACTGTTCTCGTTAAACCCCACCATGACCATCGGTCGCCAGGTTAAAGAAGCGATTTATGTGTCGTGTAAGCGCCGATATTACCAAACTAAGAGTGACCTCAAATACGATTTGCAAACAGAACGAATTGACCTGGACACTTACAAGGAAAAACTAGCACAAGCCAAGGAAACTTACAAAGCTAAAACGACTAAGGCGGCGGTCCATGCAAAAACGCTAGAAATCCTTAACTTCATTGGCATTGACCAAGCTGAAAAGCGCTTAAAGGCCTTTCCAAGTGAGTTTTCCGGGGGAATGCGGCAACGGATTGTGATCGCGATTGCCGTAGCCACCGAACCGGATTTAATTATTGCCGATGAACCGACCACGGCGTTGGATGTGACCATCCAAGCGAAGGCGTTGAACCTAATTAAACAGCTGCGTGACCTGCTCAACATTACCATTATTTTTATTAGTCACAACATCTCGTTAATTGCTAATTTCTGTGACTTTGTATACGTAATGTACGCTGGTCGATTAGTGGAAAAGGGCTTAGTTGAGGAAATCTTTACCAATCCGGTGCACCCCTACACTTGGGCATTAATGGCTTCTATCCCTGAAGGTACGGACAAAAACGCTCCGCTAACCTCAATTCCTGGGTATATTCCCAATATGTTATCCCCGCCTAAGGGCGATGCCTTTGCAGCGCGGAACCAGTTTGCACTTGCCATTGATTTCGAGCACCAACCGCCGTTCTTTGATGTAACGGAAACTCATAAGGCCGCTACCTGGCTACTCCATCCCCAAGCACCGAAGGTAGAGATGCCCGCCGATGTCAAGGAAAAGATTGCCATTACCCGCAAAGCACTCGCCATTAAAATGCCATCTCAACCTGTAAAACAACCAAAAAGCAATGGAAACAAAAAACAAAAACAAAATCCGCTCGTTAATGTTAAAGCCTTGAGCATGTTGTTCAAGGTCCGCGGTAGCTTCTTTAAAGCCTTAGATGAAATTGACTTTACCGTCAATGAAGGTGACTTCTTTGGGGTCATTGGCGAATCGGGTAGTGGTAAGTCAACCACGGGGAAGTGTTTAATCCGGTTAAATATCCCGAGTGGCGGTAAGGTGGAAATTGCTAACCACCTTATTTCTGGTAAAAAACTAACGCGCGAAAACGACCACTGGTTAAAGCAAAACGTGCAGATGGTCTTTCAAGACCCATACTCTTCTTTAAACCCTACCAAGAATGTTTTAACGGTCATTTCCGAACCGTTAGTAATTACCAAAACGGTGTATGGTGAGGTAAAGGAATATCTTAAAACGCTCGCTAAACTGTCCTTTAAAACCAAGAAGGAATTGTTAAGGGAAGACTTTGAACTGGAAACCCAGTTTTACGAAAAGTTCTTTTCAAAGGTACTGTTTCACTTAGAAACTACGATTAACAAGTTTGCCCTACTCCAAGAGAGTAATAACAATAGTTCAGCCGAGTTAGCACAAACCATTTTGGGTCACACTGATGATCTAATTGAAGCATTACGGCAAGAGTTCGGTCTGGTCTATGAGTTTTCCTCATCCCAAAGCGAACCGCTACAAAAGGCCCTCAAAGACAAACAGGAAACCTTAGCACAGGACACGATTGACAAGTTAAAGCAAGAGCTGTATACAACGCAGCAAAAAGCTAAAGTTTCCACCCAGGCCTTTGCTACTTGA", "aa": "MSYISQDPLFSLNPTMTIGRQVKEAIYVSCKRRYYQTKSDLKYDLQTERIDLDTYKEKLAQAKETYKAKTTKAAVHAKTLEILNFIGIDQAEKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKALNLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGRLVEKGLVEEIFTNPVHPYTWALMASIPEGTDKNAPLTSIPGYIPNMLSPPKGDAFAARNQFALAIDFEHQPPFFDVTETHKAATWLLHPQAPKVEMPADVKEKIAITRKALAIKMPSQPVKQPKSNGNKKQKQNPLVNVKALSMLFKVRGSFFKALDEIDFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKVEIANHLISGKKLTRENDHWLKQNVQMVFQDPYSSLNPTKNVLTVISEPLVITKTVYGEVKEYLKTLAKLSFKTKKELLREDFELETQFYEKFFSKVLFHLETTINKFALLQESNNNSSAELAQTILGHTDDLIEALRQEFGLVYEFSSSQSEPLQKALKDKQETLAQDTIDKLKQELYTTQQKAKVSTQAFAT", "aa_hexdigest": "aab74dd531390d916123f74a69a27b0a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 110, "aa_cov": 0.19332161687170474, "hmm_cov": 0.8545454545454545, "evalue": 2.7793934214366203e-28, "score": 94.72212982177734, "start": 335, "stop": 444 } ], "seq_stats": { "molecular_weight": 64077.05650000013, "isoelectric_point": 8.75604763031006 }, "id": "CNOCLNGIAL_509", "locus": "GCA000143945_00474" }, { "type": "cds", "sequence": "contig_1", "start": 268559, "stop": 269320, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACAAACTAAGCAGAACTTAAAAGCGTACCGTGCCCAAATGGCGGAAGAGTTGCAAAACAAACCACGGATTTACCTCAATGCATGGTTGTTAACAACGAAGAACTACATTAAGGATTCACGCCAAAACACGCAGTTAACTGACGATGTCTTTGCTTTTTCTTACAACGACATGGTCGACAAAAAGCGCCGCTTAGTGTTAGTGTTATCGGAGTATTACAAGGCGTTGCCGTTCTTTTATGACAATTGGATCCACCAGAACGCCGATCGTTTTGATGAATTGACCAACGCAGTCTTCTTTGACTTGATTGATGTGGTGATTGCCTTAAACCGTGATTTTGCTAATGTGGAGAGTGACGCTAAGGCCGAGTTAATCCGTTTTGTTCAGTTTATCCGTCGCTTGTGTGACTTACGCTTTGCTGCTTTGAAAAAGAGTTTTAAGAAGCAAACTAACTATAGTTTTGACTTTAACCGCGAAACCGAGTTGTTATACGCCAACAGCTGTTATGACATTAAGGAGCTCCCCCAAGTAATCCAACCATACTGGGAAAAGCTCTTTAGTGATGCTAACTATGACAAGATTGCCAAATCAGTGCAGGAACTCAACGATATTATTAGCACTGACATTGAAAAGGCATCGAATATAGCCAGTGAAATTAATACCAAGATTAGTAGTTTTAAAACTGAAATTGCGGAGTTAAAAGCTACCTTTAAAACAGAAAAAAAGCAGAAGATCACAGTGCCCAAATTACTGGCTTAA", "aa": "MQQTKQNLKAYRAQMAEELQNKPRIYLNAWLLTTKNYIKDSRQNTQLTDDVFAFSYNDMVDKKRRLVLVLSEYYKALPFFYDNWIHQNADRFDELTNAVFFDLIDVVIALNRDFANVESDAKAELIRFVQFIRRLCDLRFAALKKSFKKQTNYSFDFNRETELLYANSCYDIKELPQVIQPYWEKLFSDANYDKIAKSVQELNDIISTDIEKASNIASEINTKISSFKTEIAELKATFKTEKKQKITVPKLLA", "aa_hexdigest": "ab870040432330fe87e8919ec62d1029", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29819.714900000024, "isoelectric_point": 8.599840736389162 }, "id": "CNOCLNGIAL_510", "locus": "GCA000143945_00475" }, { "type": "cds", "sequence": "contig_1", "start": 269563, "stop": 269709, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCCACCCGGTCGAAGGTGATCTCAAGAGTGAACTCAAAACACGCTTAAAGTCGTTTGGGGTGATTAACTTTGAGTACAAACGGGCTGTGCGTGAATCCCGTGTCTTTCGCTTAGTCCACTTTGGTCATGACGTGATGAAGTGA", "aa": "MFHPVEGDLKSELKTRLKSFGVINFEYKRAVRESRVFRLVHFGHDVMK", "aa_hexdigest": "bb6849b501534a7bde8abeb0c0c6821b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5735.6476, "isoelectric_point": 9.986491203308105 }, "id": "CNOCLNGIAL_511", "locus": "GCA000143945_00476" }, { "type": "cds", "sequence": "contig_1", "start": 269740, "stop": 270309, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "nt": "ATGCGTAACAAGGTGTATGAAGCATTAGACAGTGTGGGCCTCAAACGCGAGCACGCTTATCGCTATCCGCACGAGTTTTCTGGGGGTCAGCGGCAACGGATTGCGATTGCCCGCGCTTTAATTACCAAACCTAAGTTAATTATTGCAGATGAGCTTATTAGTGCTTTGGATGTATCCATTCAAGCGCAGGTGATTAACATCTTGAAGGACTTGGCTAAAAAACACAACTTAACGGTGCTCTTTATTGCCCACGACCTTTCGATGGTACAAACAGTGTGCAACCGTTTAATTATTATGCACCGCGGCAAAATTGTGGAACGGGGTAGTACGGATGAAATCTTCGCGCACCCGGTCCACCCTTACACCCGTTCCTTGATTAAGGCATCGCCAAAGTTGAGTAAGATTAACATTGACCTAGCCTCCTTTGACGAGAAGTTTACGTATGACAGTGATTATTCACTCACGAATATGCCAAGCTTCTTGAAGGTGCCAAATACCCAAGAACACGAGTTGTACTGTACTCAAGGCCAGTTTGACAGTTGGATCAAAGGAGCAAGTCGGATAAATTAA", "aa": "MRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSTDEIFAHPVHPYTRSLIKASPKLSKINIDLASFDEKFTYDSDYSLTNMPSFLKVPNTQEHELYCTQGQFDSWIKGASRIN", "aa_hexdigest": "f94b1f67f71eb4fdf6171244cd681cf4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PJB1", "query_cov": 0.9417989417989417, "subject_cov": 0.9888888888888889, "identity": 0.6459999999999999, "score": 241.0, "evalue": 1.35e-78, "valid": false, "product": "Phosphate ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A4R0XTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A4R0XTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "CNOCLNGIAL_512", "locus": "GCA000143945_00477" }, { "type": "cds", "sequence": "contig_1", "start": 270365, "stop": 270778, "strand": "+", "frame": 2, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180", "KEGG:K02867", "RefSeq:WP_010874576.1", "SO:0001217", "UniParc:UPI0000165F90", "UniRef:UniRef100_P75550", "UniRef:UniRef50_P47327", "UniRef:UniRef90_P75550" ], "nt": "ATGGCAAAAAAGACTATTACAAGAATCGCTAAGATTAACCTCTTAGGCGGGCAAGCAAAGCCCGGTCCCGCACTCGCATCTGTTGGGATTAACATGGGTGAATTCACCAAACAATTTAACGAAAAAACCAAGGACAAACAGGGCGAAATGATCCCCTGTGTGATCACCGCTTACAACGATAAGTCGTTCGACTTTATCTTAAAAACTACCCCAGTTAGCATCTTGCTTAAGCAGGCTGCAAAACTGGAAAAGGGGGCGAAAAACGCCAAAACAATTGTGGGTAAGATTACGATGGCCAAGGCCAAGGAAATTGCGCAGTACAAGTTAGTGGATCTGAACGCTAACACCGTGGAAGCAGCCCTTAAAATGGTTTTAGGTACTGCTAAACAAATGGGAATCGAGGTGATCGAATAA", "aa": "MAKKTITRIAKINLLGGQAKPGPALASVGINMGEFTKQFNEKTKDKQGEMIPCVITAYNDKSFDFILKTTPVSILLKQAAKLEKGAKNAKTIVGKITMAKAKEIAQYKLVDLNANTVEAALKMVLGTAKQMGIEVIE", "aa_hexdigest": "053c87ebf539a426ad2f8de866f82280", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F90", "ncbi_nrp_id": "WP_010874576.1", "uniref100_id": "UniRef100_P75550", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F90", "RefSeq:WP_010874576.1", "UniRef:UniRef100_P75550" ] }, "ips": { "uniref100_id": "UniRef100_P75550", "uniref90_id": "UniRef90_P75550" }, "psc": { "uniref90_id": "UniRef90_P75550", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_P47327", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P47327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47327" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "CNOCLNGIAL_513", "locus": "GCA000143945_00478" }, { "type": "cds", "sequence": "contig_1", "start": 270778, "stop": 271095, "strand": "+", "frame": 1, "gene": null, "product": "Large ribosomal subunit protein uL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZQ2" ], "nt": "ATGGCTAAACTATCAAAGAAAATGAAGATAGCTGTTGGTTTGGTCGATAAAACCAAGCTGTACCCGCTCCAAGAAGCAGTTGATTTAGTCAAAAAAACTTCAATTACCAAATTTAATGGCTCAGTTGATATTGCCGTTAGCCTTAATTTAGACACCACCAAGGCGGAACAACAGCTCCGCGGTGCGATTGCTTTTCCTCACAGTGTTGGCAAACCAATCCGTATTCTAGCGATCACCGATGATGAAAAGGCGGCACTGGAAGCAGGTGCTGACTTTGTTGGTGGCATTGACAAGATCAATGACATTAAAAACGGTTGA", "aa": "MAKLSKKMKIAVGLVDKTKLYPLQEAVDLVKKTSITKFNGSVDIAVSLNLDTTKAEQQLRGAIAFPHSVGKPIRILAITDDEKAALEAGADFVGGIDKINDIKNG", "aa_hexdigest": "5b19d4bfed94ee26523fc0082d49746b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZQ2", "query_cov": 1.0, "subject_cov": 0.8467741935483871, "identity": 0.514, "score": 101.0, "evalue": 1.38e-25, "valid": false, "product": "Large ribosomal subunit protein uL1", "uniref50_id": "UniRef50_A0A448ZZQ2", "kegg_orthology_id": "K02863" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZQ2" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [], "id": "CNOCLNGIAL_514", "locus": "GCA000143945_00479" }, { "type": "cds", "sequence": "contig_1", "start": 271132, "stop": 271458, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "nt": "ATGGCCGCATTAGGTAAACTCGGGAAGCTGTTAGGTACCAAGGGTTTAATGCCTAACCCAAAAACAGAAACCGTTACTGATGATGTTCCAGCTGCTGTACGCGCTTACAAGAAGGGGAAAAAGGAATACCGCGCTGACAGCTTTGGTAACATCCACATGTCCTTAGGCCGCGTGGACAGTGCGTCCAACCACTTGGTTGAAAACGCTTTGGCACTGCTTGATCTCATTAAATCACGTAAACCAGCAACCGTTAAAGGAATTTACATTAAGAACATTGCCTTGACCACTACCATGGGTCCGAGCTTAAAGGTTAAGCTCCCTGATTAA", "aa": "MAALGKLGKLLGTKGLMPNPKTETVTDDVPAAVRAYKKGKKEYRADSFGNIHMSLGRVDSASNHLVENALALLDLIKSRKPATVKGIYIKNIALTTTMGPSLKVKLPD", "aa_hexdigest": "58ffddf1d8b4f0081ae864d6799677f9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2P6FDS1", "query_cov": 0.9629629629629629, "subject_cov": 0.9629629629629629, "identity": 0.5770000000000001, "score": 117.0, "evalue": 4.06e-32, "valid": false, "product": "Ribosomal protein", "uniref50_id": "UniRef50_A0A2P6FDS1", "kegg_orthology_id": "K02863" }, "pscc": { "uniref50_id": "UniRef50_A0A2P6FDS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "product": "Ribosomal protein" }, "genes": [], "id": "CNOCLNGIAL_515", "locus": "GCA000143945_00480" }, { "type": "cds", "sequence": "contig_1", "start": 271458, "stop": 271682, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01195.25" ], "nt": "ATGGACAAGTTGCGCCTCGTTGTTGGTTTAGGTAATTTAGGTAAGCAGTACGCTGAAACCCGTCATAACGCGGGGTTTAAGGTAATTGATCGTTTACTAAGCCTTTACCATGTCCAGTTAGAGGAACGCAACAACTTAGGTGAATTCATCCTTTTAAGAAAACATAAGGTAGTGTTAGCTAAGCCAAACACTTACATGAACCACAGTGGTAAGTTTGTTAAATGA", "aa": "MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLAKPNTYMNHSGKFVK", "aa_hexdigest": "1cbf33d032f4bf75a9e1bde68f8f53dd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01195.25", "name": "Pept_tRNA_hydro", "length": 71, "aa_cov": 0.9324324324324325, "hmm_cov": 1.0, "evalue": 2.0646392117911025e-24, "score": 81.8519287109375, "start": 6, "stop": 74 } ], "seq_stats": { "molecular_weight": 8560.959699999996, "isoelectric_point": 10.070106697082519 }, "id": "CNOCLNGIAL_516", "locus": "GCA000143945_00481" }, { "type": "cds", "sequence": "contig_1", "start": 271719, "stop": 272024, "strand": "+", "frame": 3, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "GO:0005737", "GO:0006412", "SO:0001217", "UniRef:UniRef50_P47329", "UniRef:UniRef90_P78034" ], "nt": "ATGGTGGTTTATGATGAATTAGCCTTCCCGTTAGGTACTGTTCGACTAAAAATGCAGGGTTCAGCCAACAATCATAATGGCATAAAATCAGTAATTGCGCATTTAAATACTGAACACTTCAACCGCCTCCGGTTCGGAATTAAGTCCGATAATACTAGTAACATTCTCCATGAAGTGGTAATGTCGGAGTTTACCCCAGCCGAGCGTAATTTACTTGAAACAGCGCTTACAAAAGCGATTGAAGCACTCAAGGGTTACATTGATGGTGTGACAATGCTTAAATTAATGGAAGTTTTCAATGCCTAA", "aa": "MVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHLNTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLETALTKAIEALKGYIDGVTMLKLMEVFNA", "aa_hexdigest": "15015e4edb697318eee9f8f9aaf981fa", "start_type": "ATG", "rbs_motif": null, "genes": [ "pth" ], "truncated": "5-prime", "pseudogene": { "start": 271458, "stop": 272024, "observations": { "mutation": [ 271680, 271695 ], "stop": [ 271680, 271695 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78034", "query_cov": 1.0, "subject_cov": 0.5372340425531915, "identity": 1.0, "score": 198.0, "evalue": 4.05e-63, "gene_start": 1, "gene_end": 101, "reference_start": 88, "reference_end": 188, "reference_sequence": "MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLAKPNTYMNHSGKFVKWACQNWNIKPDKVMVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHLNTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLETALTKAIEALKGYIDGVTMLKLMEVFNA" }, "paralog": false, "identity": 0.9893617021276596, "score": 367.0, "evalue": 2.77e-132, "description": "Internal stop codon at 271680, 271695. Nonsense mutation around 271680, 271695.", "psc": { "uniref90_id": "UniRef90_P78034", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_P47329", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045", "GO:0005737", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78034", "EC:3.1.1.29", "UniRef:UniRef50_P47329", "COG:COG0193", "COG:J", "GO:0004045", "GO:0005737", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P47329", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47329" ], "product": "Peptidyl-tRNA hydrolase" } }, "id": "CNOCLNGIAL_517", "locus": "GCA000143945_00482" }, { "type": "cds", "sequence": "contig_1", "start": 272017, "stop": 272226, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAACACAATACATAGCTGGAGTATCAGGTGGTCCTGATTCAATGTTGTTGTTAAAACTGTACCACAAGAAGATTGCCTGTGTTGTACACGTTAACTACAATAAGCGGACTACTGCACTCCGCGACCAAAAAATGGTCGAAGAATACTGTAAACAGCTCAAAGTGCCCCTCATTGTGCACACGGTACCTGAAGATACCGTTTGA", "aa": "MPKTQYIAGVSGGPDSMLLLKLYHKKIACVVHVNYNKRTTALRDQKMVEEYCKQLKVPLIVHTVPEDTV", "aa_hexdigest": "59eb6744c086998d12ffa2eacba17473", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7841.224099999999, "isoelectric_point": 9.230728340148925 }, "id": "CNOCLNGIAL_518", "locus": "GCA000143945_00483" }, { "type": "cds", "sequence": "contig_1", "start": 272353, "stop": 272451, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGTTGGACGCACGCAAGCGTGCCATGTATTATGGCATTAAAACACGTGGCGAATTGTATGGGATGAAGGTCTATCGTCCCTTTATTAAGTACTGA", "aa": "MQLDARKRAMYYGIKTRGELYGMKVYRPFIKY", "aa_hexdigest": "ced749f3222818e496a77cb11ec9c2b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3947.6959000000015, "isoelectric_point": 10.087642097473143 }, "id": "CNOCLNGIAL_519", "locus": "GCA000143945_00484" }, { "type": "cds", "sequence": "contig_1", "start": 272608, "stop": 272886, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCGCGTCATTGGTCAAAAGCTCTTTTCCTTAATGAAACGTGCAAAACAGTGGTTAGCTCATCCTGATGTACGTGAATTAAAGCGTTACCCACTTCCCGATCAACGTCAGTTGGTTTACAGCTTTTTAATTACCCACCACATTGATGTAACGGGGGACAAGATTGATGCCATTTTAGACTTTATCCAACCGTTTCAACAAAAGCATTACCGTCTCAAGGATGAGATCTTCTTATCCATTAAAGACGAACGGCTAACTTTACTGTACAAGAGTTAG", "aa": "MNRVIGQKLFSLMKRAKQWLAHPDVRELKRYPLPDQRQLVYSFLITHHIDVTGDKIDAILDFIQPFQQKHYRLKDEIFLSIKDERLTLLYKS", "aa_hexdigest": "e986e4fbe5ec5270d2f8cd4b97801e66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11109.88659999999, "isoelectric_point": 9.643519401550293 }, "id": "CNOCLNGIAL_520", "locus": "GCA000143945_00485" }, { "type": "cds", "sequence": "contig_1", "start": 272887, "stop": 273825, "strand": "+", "frame": 1, "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "db_xrefs": [ "COG:COG1493", "COG:T", "EC:2.7.11.-", "EC:2.7.4.-", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109", "KEGG:K06023", "RefSeq:WP_010874580.1", "SO:0001217", "UniParc:UPI00001121FD", "UniRef:UniRef100_P75548", "UniRef:UniRef50_P75548", "UniRef:UniRef90_P75548" ], "nt": "ATGAAAAAGTTATTAGTCAAGGAGTTAATCGAACAATTTCAGGATTGTGTTAACCTCATTGATGGGCACACTAACACCAGTAATGTAATTAGAGTACCCGGATTAAAGCGGGTGGTGTTTGAAATGCTGGGGTTGTTTTCCAGCCAAATTGGCTCGGTAGCCATTTTGGGCAAACGCGAATTTGGCTTTTTGAGTCAAAAAACGCTGGTTGAACAACAGCAAATTCTCCACAACTTACTCAAACTCAACCCACCAGCGATTATTTTGACCAAGTCCTTTACTGACCCCACGGTACTTTTACAGGTTAACCAGACTTATCAGGTACCGATCCTAAAGACCGATTTCTTTTCCACGGAACTTTCCTTTACGGTCGAAACCTACATTAACGAACAGTTTGCTACTGTTGCACAAATTCACGGCGTCTTGCTGGAAGTTTTTGGTGTAGGTGTCTTATTAACTGGTCGCAGTGGCATTGGTAAATCGGAGTGTGCCTTAGATCTAATTAACAAGAACCACCTCTTTGTCGGTGATGATGCGATTGAAATATACCGTTTGGGTAATCGCTTGTTTGGTCGCGCGCAGGAAGTAGCGAAAAAGTTTATGGAAATCCGTGGTCTTGGTATTATTAACGTCGAGCGCTTTTACGGATTGCAAATTACTAAACAGCGCACCGAAATCCAGTTAATGGTGAACTTACTGAGTTTAGAAAAACAAACGACCGTGACCTTTGAGCGTTTAGGTACCGAATTGAAAAAACAACGTTTACTCGGAGTTGATTTGAGCTTTTATGAAATTCCGATTTCCCCCGGGCGGAAGACGAGTGAAATCATTGAAAGCGCTGTAATTGATTTTAAACTTAAGCACAGTGGCTATAACTCTGCTTTAGACTTTATTGAAAACCAGAAGGCCATTTTAAAACGTAAAAAAGATGAATCCTAG", "aa": "MKKLLVKELIEQFQDCVNLIDGHTNTSNVIRVPGLKRVVFEMLGLFSSQIGSVAILGKREFGFLSQKTLVEQQQILHNLLKLNPPAIILTKSFTDPTVLLQVNQTYQVPILKTDFFSTELSFTVETYINEQFATVAQIHGVLLEVFGVGVLLTGRSGIGKSECALDLINKNHLFVGDDAIEIYRLGNRLFGRAQEVAKKFMEIRGLGIINVERFYGLQITKQRTEIQLMVNLLSLEKQTTVTFERLGTELKKQRLLGVDLSFYEIPISPGRKTSEIIESAVIDFKLKHSGYNSALDFIENQKAILKRKKDES", "aa_hexdigest": "fae0e685a2d839a331d0f12f1b2ecabd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001121FD", "ncbi_nrp_id": "WP_010874580.1", "uniref100_id": "UniRef100_P75548", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001121FD", "RefSeq:WP_010874580.1", "UniRef:UniRef100_P75548" ] }, "ips": { "uniref100_id": "UniRef100_P75548", "uniref90_id": "UniRef90_P75548" }, "psc": { "uniref90_id": "UniRef90_P75548", "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "ec_ids": [ "2.7.11.-", "2.7.4.-" ], "uniref50_id": "UniRef50_P75548", "cog_id": "COG1493", "cog_category": "T", "kegg_orthology_id": "K06023", "go_ids": [ "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109" ] }, "pscc": { "uniref50_id": "UniRef50_P75548", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75548" ], "product": "HPr kinase/phosphorylase" }, "genes": [ "hprK" ], "id": "CNOCLNGIAL_521", "locus": "GCA000143945_00486" }, { "type": "cds", "sequence": "contig_1", "start": 273932, "stop": 274744, "strand": "+", "frame": 2, "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "db_xrefs": [ "COG:COG0682", "COG:M", "EC:2.5.1.145", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158", "SO:0001217", "UniRef:UniRef50_P47332", "UniRef:UniRef90_P75547" ], "nt": "ATGTTTGTCTTGAAGTTAATTTACTTTTACAAGATTCAAGATAACAGCTTTTACTTCTTTATCTTCATTGCAGTTTTAACAATGGTATTGGGCGCACGAGCTTGGTATTTTTTGATTGAAGCGGTTGATGGTCGTTCAAGTGGGAGCAACTTCTTTGACTTCCGCAACGGTGGGTTAGCAATCCAAGGTGGTGTGTTGTTAACTACGTTAGCGGGGATTATCTACTTTAACGTCTTTTTAAACATGAAGACGACCAAAACCAAAACCACCGCTAAACTATTAAACAACAAGAACCAGATTAAAACGGTTTATGTCGAGCGTAACATCTCAGTGTTTGTGATGTTGGATCTCATCGCTCCCTGTGTGTTAATTGGTCAAGCCATTGGTCGTTGGGGTAACTTCTTTAACGCGGAAGTTTATGGCGCAGCTTTAGTGGGTTCCAAAAATGATACCCTGTCAGCTGCTAACACCACCTGGGGCTTTTTAAGAATCTTAATGCCCAAAGTGTGGGACGGAATGTTTATCAATGGTAGCTTCCGCATTCCGCTTTTCTTAATCGAATCGTTCTTTAACACGATCTTCTTTGTCTTTATCTACTTTGTAATGGACCACATTAAGGGGATCCGCAGTGGGACAATTGGCTTTAGTTACTTCTTAGCGACTGGCATAGTTCGCTTAATCTTGGAAACGCAGCGTGATGAAGCGTTTAAGTACAATACCTCGATTGTGTTTAGTGCACTCTTAATTTTGGTGGGAATAGTAGGGATTATTTACTGTCAAACGTTAGCAATTAAATTGCGTGGTTATTTCTGA", "aa": "MFVLKLIYFYKIQDNSFYFFIFIAVLTMVLGARAWYFLIEAVDGRSSGSNFFDFRNGGLAIQGGVLLTTLAGIIYFNVFLNMKTTKTKTTAKLLNNKNQIKTVYVERNISVFVMLDLIAPCVLIGQAIGRWGNFFNAEVYGAALVGSKNDTLSAANTTWGFLRILMPKVWDGMFINGSFRIPLFLIESFFNTIFFVFIYFVMDHIKGIRSGTIGFSYFLATGIVRLILETQRDEAFKYNTSIVFSALLILVGIVGIIYCQTLAIKLRGYF", "aa_hexdigest": "d809685d1086983575577ed9ac438c26", "start_type": "ATG", "rbs_motif": null, "genes": [ "lgt" ], "truncated": "both", "pseudogene": { "start": 273815, "stop": 274984, "observations": { "mutation": [ 273845, 274742 ], "stop": [ 273845, 274742 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75547", "query_cov": 1.0, "subject_cov": 0.6940874035989717, "identity": 1.0, "score": 521.0, "evalue": 7.79e-185, "gene_start": 1, "gene_end": 270, "reference_start": 40, "reference_end": 309, "reference_sequence": "MNPSVSSRPPWSTAFYLGPGFPIQWYGIIVAIGIAFGILMFVLKLIYFYKIQDNSFYFFIFIAVLTMVLGARAWYFLIEAVDGRSSGSNFFDFRNGGLAIQGGVLLTTLAGIIYFNVFLNMKTTKTKTTAKLLNNKNQIKTVYVERNISVFVMLDLIAPCVLIGQAIGRWGNFFNAEVYGAALVGSKNDTLSAANTTWGFLRILMPKVWDGMFINGSFRIPLFLIESFFNTIFFVFIYFVMDHIKGIRSGTIGFSYFLATGIVRLILETQRDEAFKYNTSIVFSALLILVGIVGIIYCQTLAIKLRGYFWTYFFLYGWYKVAAFFTTLFMKDRTQACSSKFAFYEKSLPEKERSFFQLKYYNDVLPPKIYRLYDHEMLMFDKLEAVPEA" }, "paralog": false, "identity": 0.9948586118251928, "score": 775.0, "evalue": 1.71e-287, "description": "Internal stop codon at 273845, 274742. Nonsense mutation around 273845, 274742.", "psc": { "uniref90_id": "UniRef90_P75547", "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "ec_ids": [ "2.5.1.145" ], "uniref50_id": "UniRef50_P47332", "cog_id": "COG0682", "cog_category": "M", "go_ids": [ "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75547", "EC:2.5.1.145", "UniRef:UniRef50_P47332", "COG:COG0682", "COG:M", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ] }, "pscc": { "uniref50_id": "UniRef50_P47332", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47332" ], "product": "Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase" } }, "id": "CNOCLNGIAL_522", "locus": "GCA000143945_00487" }, { "type": "cds", "sequence": "contig_1", "start": 274802, "stop": 274984, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGATAGGACACAGGCTTGTTCTAGTAAGTTTGCATTTTACGAAAAGAGCTTACCTGAAAAGGAACGTTCCTTCTTTCAGTTGAAGTACTACAACGATGTTTTACCACCGAAAATTTACCGTTTGTACGACCATGAAATGCTCATGTTTGACAAGCTAGAAGCAGTGCCGGAAGCTTAG", "aa": "MKDRTQACSSKFAFYEKSLPEKERSFFQLKYYNDVLPPKIYRLYDHEMLMFDKLEAVPEA", "aa_hexdigest": "6ce78c1ca986456bc495a774dc51c15c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7287.329, "isoelectric_point": 6.553331184387207 }, "id": "CNOCLNGIAL_523", "locus": "GCA000143945_00488" }, { "type": "cds", "sequence": "contig_1", "start": 275013, "stop": 275432, "strand": "+", "frame": 3, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_014325410.1", "SO:0001217", "UniParc:UPI0001DD64F2", "UniRef:UniRef100_A0AB38W7X5", "UniRef:UniRef50_P92532", "UniRef:UniRef90_P47333" ], "nt": "ATGGCTACAATCGCACAATTAATTAGAAAACCGCGGAAAAAGAAGAAGGTTAAATCTAAATCACCCGCTTTACATTACAACCTCAATTTACTCAACAAAAAGGTAACCAACGTGTATTCACCACTTAAACGTGGTGTGTGTACCCGGGTTGGTACCATGACTCCAAAAAAACCGAACTCTGCTTTGCGCAAATATGCCAAGGTAAGACTTACTAACGGTTTTGAGGTGTTGGCTTATATCCCTGGTGAAGGACACAACCTCCAAGAACACAGTGTAACGCTACTCCGTGGCGGTCGGGTGAAAGACCTCCCTGGTGTGCGTTACCACATTGTTCGTGGTACCTTAGACACTGTTGGTGTGGAAAAACGCCGTCAACAACGTTCAGCTTACGGGGCTAAGAAACCAAAAGCCAAGTCCTAA", "aa": "MATIAQLIRKPRKKKKVKSKSPALHYNLNLLNKKVTNVYSPLKRGVCTRVGTMTPKKPNSALRKYAKVRLTNGFEVLAYIPGEGHNLQEHSVTLLRGGRVKDLPGVRYHIVRGTLDTVGVEKRRQQRSAYGAKKPKAKS", "aa_hexdigest": "a3ad0284a3ab459e11c3b8aec2ce0e50", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64F2", "ncbi_nrp_id": "WP_014325410.1", "uniref100_id": "UniRef100_A0AB38W7X5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64F2", "RefSeq:WP_014325410.1", "UniRef:UniRef100_A0AB38W7X5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7X5", "uniref90_id": "UniRef90_P47333" }, "psc": { "uniref90_id": "UniRef90_P47333", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "genes": [ "rpsL" ], "id": "CNOCLNGIAL_524", "locus": "GCA000143945_00489" }, { "type": "cds", "sequence": "contig_1", "start": 275486, "stop": 275791, "strand": "+", "frame": 2, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "nt": "ATGCGAAAAAATCGTGCTCCCAAACGGACTGTTTTACCCGATCCCGTTTTTAACAATACGCTGGTAACCCGGATTATTAACGTCATCATGGAAGATGGCAAGAAGGGTTTAGCACAACGAATCTTATACGGTGCTTTTGATCTCATTGAACAACGCACTAAAGAAAAACCCTTAACCGTGTTTGAACGCGCTGTGGACAACGTGATGCCGCGCTTGGAACTCCGGGTACGGCGGATTGCGGGATCCAACTATCAGGTACCTACCGAAGTACCTCAAGACCGCAAGATTGCTCTAGCACTCCGTTGA", "aa": "MRKNRAPKRTVLPDPVFNNTLVTRIINVIMEDGKKGLAQRILYGAFDLIEQRTKEKPLTVFERAVDNVMPRLELRVRRIAGSNYQVPTEVPQDRKIALALR", "aa_hexdigest": "61a6be463fd1b42d2fdc4b4247f07a1e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A381KMK1", "query_cov": 0.9900990099009901, "subject_cov": 0.8264462809917356, "identity": 0.52, "score": 113.0, "evalue": 1.5e-30, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A381KMK1", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A381KMK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "product": "Ribosomal protein S7" }, "genes": [], "id": "CNOCLNGIAL_525", "locus": "GCA000143945_00490" }, { "type": "cds", "sequence": "contig_1", "start": 275798, "stop": 275953, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00177.27" ], "nt": "ATGTTTGCGCGTAAGCGTCATGAAAAAACGATGTTAGAGAAGATTGCTAACGAAATTATTGATGCATCGAATAATACTGGTGCAGCCATTAAAAAGAAGGACGATACGCATAAGATGGCTGAAGCCAACAAGGCCTTTGCCCACATGCGTTGGTAA", "aa": "MFARKRHEKTMLEKIANEIIDASNNTGAAIKKKDDTHKMAEANKAFAHMRW", "aa_hexdigest": "7ac9d132e00cba7d2f89c12b1637683f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 0.8627450980392157, "subject_cov": 0.88, "identity": 0.75, "score": 72.8, "evalue": 4.05e-16, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00177.27", "name": "Ribosomal_S7", "length": 43, "aa_cov": 0.8431372549019608, "hmm_cov": 1.0, "evalue": 3.3643711877676678e-18, "score": 61.14462661743164, "start": 2, "stop": 44 } ], "seq_stats": { "molecular_weight": 5899.768200000001, "isoelectric_point": 9.774519157409667 }, "id": "CNOCLNGIAL_526", "locus": "GCA000143945_00491" }, { "type": "cds", "sequence": "contig_1", "start": 275976, "stop": 276335, "strand": "+", "frame": 3, "gene": null, "product": "Elongation factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S3U2I5" ], "nt": "ATGGCGCGTACAGTAGATTTAATTAACTTCCGTAATTTCGGAATTATGGCCCACATTGATGCGGGTAAAACCACCACCTCAGAGCGAATTTTATTTCACTCAGGCCGAATCCACAAGATTGGGGAAACCCATGACGGGGAGTCCGTGATGGACTGGATGGAGCAGGAAAAGGAACGCGGTATTACGATTACCTCCGCAGCTACTTCAGTTAGTTGGAAGAACTGCAGCTTAAACCTAATTGACACCCCCGGTCACGTTGACTTTACCGTAGAAGTAGAACGCAGTCTCCGTGTCCTTGATGGTGCCATTGCTGTGTTAGATGCTCAAATGGGGGTAGAACCACAAACCGAAACGGTGTGA", "aa": "MARTVDLINFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVSWKNCSLNLIDTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETV", "aa_hexdigest": "66d3b63bb2431530da923cf91c9296ef", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2S3U2I5", "query_cov": 0.9663865546218487, "subject_cov": 0.8984375, "identity": 0.7909999999999999, "score": 184.0, "evalue": 2.62e-58, "valid": false, "product": "Elongation factor", "uniref50_id": "UniRef50_A0A2S3U2I5" }, "pscc": { "uniref50_id": "UniRef50_A0A2S3U2I5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S3U2I5" ], "product": "Elongation factor" }, "genes": [], "id": "CNOCLNGIAL_527", "locus": "GCA000143945_00492" }, { "type": "cds", "sequence": "contig_1", "start": 276357, "stop": 278042, "strand": "+", "frame": 3, "gene": null, "product": "Elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHN5" ], "nt": "GTGCCCCGGATTATCTTTGTCAATAAGATGGACAAAACGGGGGCTAACTTCGAGCGTTCTGTACAATCGATTCAACAGCGCTTAGGAGTAAAGGCGGTACCTATCCAATTACCAATTGGTGCGGAAAACGACTTTAACGGCATTATTGACCTGATTGAAGAGAAAGTTTACTTCTTTGATGGTGGTAAGGAAGAAAAGGCCGAGGAAAAACCAATTCCCGACCAGTTTAAGGATCAGGTCAAACAAATGCGTGCGCACCTAGTGGAAGAGGTGGCTAACTTTGACGATCAGTTAATGGCTGATTACCTTGAAGGTAAGGAAATTAGTATTGCCGACATCAAGCGCTGTATCCGCAAAGGGGTAATAGGTTGTCAGTTCTTCCCGGTGCTTTGTGGTTCTGCCTTTAAAAACAAGGGGATCAAACTGTTATTGGATGCTGTAGTAGATTTCTTACCTTCCCCAGTTGACGTACCGCAAGCCAAGGCTTATGGTGAAGATGGCAATGAGGTGTTAATCTCGGCTAGTGATGATGCACCGTTTGTTGGTTTAGCCTTTAAGGTAGCGACTGACCCATTTGTGGGACGTTTAACCTTTGTCCGCGTTTACTCCGGAGTACTTAAGTCCGGTTCTTACGTTAAGAATGTAAGAAAGAACAAAAAGGAACGGGTATCACGTTTGGTAAAAATGCACGCGCAGAACCGCAATGAAATCGAGGAAATTCGTGCTGGTGATATCTGTGCTATTATTGGATTAAAGGACACCACTACTGGAGAAACCCTAGTTGATGACAAGATTGATGTCCAACTAGAAGCGATGCAGTTCGCACAACCCGTGATCTCTTTAGCGGTGGAACCAAAAACCAAGGCAGATCAGGAAAAAATGTCGATTGCACTCTCGAAGTTAGCGGAAGAAGATCCGACCTTTAAGACCTTCACTGATCCCGAAACTGGGCAAACGATTATTGCGGGAATGGGTGAATTGCACCTAGACATCCTAGTGGATCGGATGCGCCGCGAGTTTAAGGTTGAAGTTAATGTGGGCGCACCACAAGTAAGCTTCCGTGAAACCTTCAATAAAGAAAGTGAAGTTGAGGGTAAATACATTAAACAATCGGGTGGCCGCGGTCAGTATGGTCATGTCAAGATTCGCTTTGAACCAAACAAGGACAAGGGCTTTGAGTTTGTCGACAAGATTGTTGGTGGTCGGATTCCGCGTGAGTACATTAAACCAGTGCAAGCGGGTTTGGAAAACGCTATGGCATCAGGTCCGCTAGCTGGTTATCCGATGATTGACATCAAAGCTACCTTGTTTGATGGTTCGTTCCACGAAGTTGATTCGAGTGAAATGGCCTTCAAGATTGCGGCATCCTTAGCTTTAAAAGAGGCGGGCAAGGTATGTAGTCCGGTACTGTTAGAGCCAATTATGGCAATTGAAGTGACGGTGCCAGAACAATACTTTGGGGACACCATGGGTGACATATCATCGAGAAGAGGTCTCATTGAGGGGACTGAACAACGTGATAACGTGCAGGTAATTAAGGCCAAAGTACCACTCAAAGAGATGTTTGGTTACGCCACTGATTTACGTTCCTTCTCTCAAGGTCGGGGTAACTATGTGATGCAGTTTAGCCATTACGCTGAAACCCCTAAGAGTGTGGTGAATGAGATAATTGCCACCAAGAAATAG", "aa": "MPRIIFVNKMDKTGANFERSVQSIQQRLGVKAVPIQLPIGAENDFNGIIDLIEEKVYFFDGGKEEKAEEKPIPDQFKDQVKQMRAHLVEEVANFDDQLMADYLEGKEISIADIKRCIRKGVIGCQFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPQAKAYGEDGNEVLISASDDAPFVGLAFKVATDPFVGRLTFVRVYSGVLKSGSYVKNVRKNKKERVSRLVKMHAQNRNEIEEIRAGDICAIIGLKDTTTGETLVDDKIDVQLEAMQFAQPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFTDPETGQTIIAGMGELHLDILVDRMRREFKVEVNVGAPQVSFRETFNKESEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQAGLENAMASGPLAGYPMIDIKATLFDGSFHEVDSSEMAFKIAASLALKEAGKVCSPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGLIEGTEQRDNVQVIKAKVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIATKK", "aa_hexdigest": "f72eaf62423e569b69770962b63c4b0f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75544", "query_cov": 1.0, "subject_cov": 0.815406976744186, "identity": 0.8859999999999999, "score": 978.0, "evalue": 0.0, "valid": false, "gene": "fusA", "product": "elongation factor G", "uniref50_id": "UniRef50_Q5SHN5", "cog_id": "COG0480", "cog_category": "J", "kegg_orthology_id": "K02355", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHN5" ], "product": "Elongation factor G" }, "genes": [], "id": "CNOCLNGIAL_528", "locus": "GCA000143945_00493" }, { "type": "cds", "sequence": "contig_1", "start": 278099, "stop": 278746, "strand": "+", "frame": 2, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904", "KEGG:K02990", "RefSeq:WP_010874585.1", "SO:0001217", "UniParc:UPI00001350BA", "UniRef:UniRef100_P75543", "UniRef:UniRef50_P47336", "UniRef:UniRef90_P75543" ], "nt": "ATGCAATACAACATTATTCTTTTAGTTGATGGTTCGCTCTCGTTAGAGCAAGCTAACCAAGTGAATGAAAAACAACAGCAAACGTTAACCAACGTTGAGGGTTTGCAAACAGAATACCTTGGTTTAAAGGAGTTGGCATACCCAATTAAGAAACAACTCTCAGCGCATTATTACCGATGGAAGTTTAGTGGTGATAACCAATCCACGAAGGACTTTAAGCGTACCGCTAACATTAACAAGCAAGTATTACGTGAACTGATTATTAACTTAGAACGTGAGTACGGCTACTTAGCTTCGATAAATCCAAAAAAGCAACAGCTGGCACTGCAAAAACGCGCTAAGTATGACGAAATCATTGCGCGGGAAAACAACCCCGAAAACCCGGATGTACCAGTAACATCAGGTTTAGCTTCCACCCAACCACGCTTATCACGTACTGAAAAGGCACAAAAACCAAAGGAAGAGCTGTGGGATGTGGTGCAAAAGATGGGAAACTTTGACAGCGTTCAAGCCAACCCATACCGCCCACGTTTCAAACGCTTTAACGCGGAACACGTTAACCAACGGCAAAACCAACAAAACAATAACAATAACCGTTTTGACCGCAACCGCAACCGTCAGCACAACCGCTTTAAGGATAAGCAATAA", "aa": "MQYNIILLVDGSLSLEQANQVNEKQQQTLTNVEGLQTEYLGLKELAYPIKKQLSAHYYRWKFSGDNQSTKDFKRTANINKQVLRELIINLEREYGYLASINPKKQQLALQKRAKYDEIIARENNPENPDVPVTSGLASTQPRLSRTEKAQKPKEELWDVVQKMGNFDSVQANPYRPRFKRFNAEHVNQRQNQQNNNNNRFDRNRNRQHNRFKDKQ", "aa_hexdigest": "873e68ea666bf750c16ec1ee7254aa62", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001350BA", "ncbi_nrp_id": "WP_010874585.1", "uniref100_id": "UniRef100_P75543", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001350BA", "RefSeq:WP_010874585.1", "UniRef:UniRef100_P75543" ] }, "ips": { "uniref100_id": "UniRef100_P75543", "uniref90_id": "UniRef90_P75543" }, "psc": { "uniref90_id": "UniRef90_P75543", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_P47336", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47336" ], "product": "Small ribosomal subunit protein bS6" }, "genes": [ "rpsF" ], "id": "CNOCLNGIAL_529", "locus": "GCA000143945_00494" }, { "type": "cds", "sequence": "contig_1", "start": 278746, "stop": 279246, "strand": "+", "frame": 1, "gene": "ssb", "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "COG:COG0629", "COG:L", "GO:0003697", "GO:0006260", "GO:0009295", "RefSeq:WP_010874586.1", "SO:0001217", "UniParc:UPI0000135FAD", "UniRef:UniRef100_P75542", "UniRef:UniRef50_P75542", "UniRef:UniRef90_P75542" ], "nt": "ATGAACCGCGTTTTTTTATTTGGTAAGCTCTCGTTTGATCCTAATAAATTGCAAACCCGTACCAACAATATTGGCGCGTCCTTTTCCTTAGCGTGCATTGATTCGAGCGGTTTTAATGACAGTAAGTCCTATATTAGGATTACTGCTTGGGGTAAAGTTGCTAGCTTTGTTTTAACTTTAAAACCCGGTGACAGTGTTTTTGTGGAGGGGAGATTGTCCACTTACAAGATGAACAACCGCAGTGATGATCCCAATTCGAAGGCCACTTACGCGCTCCAAGTTATCGCTGATAAGGTTTACCGTCCCGATGAGGAAAACAGTTTAGAGCAACCTGTTGATAAGGCTACGGTAATTGATTCACCCTTTTTAGCAGCGAAAACCAATGCTACTGAAAATGAATTAGCCCAAGCATTTCCGATTAGTCTGGATGATGAAGATGATGACATTAACCCCATTTTAAACAACGATTCCCAACTAGAGGAGGAGAGTGATGATGAATAA", "aa": "MNRVFLFGKLSFDPNKLQTRTNNIGASFSLACIDSSGFNDSKSYIRITAWGKVASFVLTLKPGDSVFVEGRLSTYKMNNRSDDPNSKATYALQVIADKVYRPDEENSLEQPVDKATVIDSPFLAAKTNATENELAQAFPISLDDEDDDINPILNNDSQLEEESDDE", "aa_hexdigest": "b6ac7ad7b813f9741825c5f23f1a44b2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135FAD", "ncbi_nrp_id": "WP_010874586.1", "uniref100_id": "UniRef100_P75542", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135FAD", "RefSeq:WP_010874586.1", "UniRef:UniRef100_P75542" ] }, "ips": { "uniref100_id": "UniRef100_P75542", "uniref90_id": "UniRef90_P75542" }, "psc": { "uniref90_id": "UniRef90_P75542", "gene": "ssb", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_P75542", "cog_id": "COG0629", "cog_category": "L", "go_ids": [ "GO:0009295", "GO:0003697", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_P75542", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75542" ], "product": "Single-stranded DNA-binding protein" }, "genes": [ "ssb" ], "id": "CNOCLNGIAL_530", "locus": "GCA000143945_00495" }, { "type": "cds", "sequence": "contig_1", "start": 279236, "stop": 279550, "strand": "+", "frame": 2, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181", "KEGG:K02963", "RefSeq:WP_010874587.1", "SO:0001217", "UniParc:UPI0000134DA9", "UniRef:UniRef100_P75541", "UniRef:UniRef50_P47338", "UniRef:UniRef90_P75541" ], "nt": "ATGATGAATAACGAACACGATAATTTCCAAAAAGAAGTTGAAACTACAACTGAAACAACCTTCAACAGAGAAGAAGGCAAAAGAATGGTACGTCCTTTATTTAAGCGTTCTAAAAAGTACTGCCGTTTTTGTGCCATTGGTCAACTTAGAATTGACTTAATTGATGATTTAGAAGCGTTAAAGCGTTTCTTGAGTCCCTACGCTAAGATTAACCCACGCCGGATTACTGGTAACTGCCAAATGCATCAGCGCCATGTAGCCAAGGCCTTAAAGCGTGCACGTTACTTAGCCTTAGTTCCGTTTGTAAAAGATTAG", "aa": "MMNNEHDNFQKEVETTTETTFNREEGKRMVRPLFKRSKKYCRFCAIGQLRIDLIDDLEALKRFLSPYAKINPRRITGNCQMHQRHVAKALKRARYLALVPFVKD", "aa_hexdigest": "13d5ab5bae3dc0a1963c684bbcdb0aa6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134DA9", "ncbi_nrp_id": "WP_010874587.1", "uniref100_id": "UniRef100_P75541", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DA9", "RefSeq:WP_010874587.1", "UniRef:UniRef100_P75541" ] }, "ips": { "uniref100_id": "UniRef100_P75541", "uniref90_id": "UniRef90_P75541" }, "psc": { "uniref90_id": "UniRef90_P75541", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_P47338", "cog_id": "COG0238", "cog_category": "J", "kegg_orthology_id": "K02963", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47338" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "CNOCLNGIAL_531", "locus": "GCA000143945_00496" }, { "type": "cds", "sequence": "contig_1", "start": 279553, "stop": 280002, "strand": "+", "frame": 1, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02939", "RefSeq:WP_010874588.1", "SO:0001217", "UniParc:UPI00001341F3", "UniRef:UniRef100_P75540", "UniRef:UniRef50_P75540", "UniRef:UniRef90_P75540" ], "nt": "ATGAAGGTAATTTTGAAGCAAGATGTTAGCAACCTAGGCAAACGCTTTGATGTGGTTGATGTTAAAGATGGCTATGCAATCCACTTTTTGTTTCCTAAAAAACTGGCTGCACCTTTAACGAAAAAGAGTTTACAAGACCGTGATTTGTTCTTAAAGAAACAACAAGAACACTATGAGATTAACAAGGCGTTAAGCCACAAGTTAAAGGAAGTAATTGAACAAACAGAACTCCACTTTAGCTTAAAGGAGCACAACGGTCGGCCATATGGCTCGATTATTACCAAGCAAATTATTAACCAAGCCCACACTAAAGGGATGGCGCTGCAAAAGTTTATGTTTAAAGACAACGTGCGCTTAGGGTTTGGGGATCACGAGATTACCTTACACATCTTTGAAGATACAACCGCAGTTTTAAAAGTTAAGGTAACCCCGGATAATGGTGTCAAATAA", "aa": "MKVILKQDVSNLGKRFDVVDVKDGYAIHFLFPKKLAAPLTKKSLQDRDLFLKKQQEHYEINKALSHKLKEVIEQTELHFSLKEHNGRPYGSIITKQIINQAHTKGMALQKFMFKDNVRLGFGDHEITLHIFEDTTAVLKVKVTPDNGVK", "aa_hexdigest": "d5dbdcbeda3b0120f27d9dcdc51fef08", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001341F3", "ncbi_nrp_id": "WP_010874588.1", "uniref100_id": "UniRef100_P75540", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341F3", "RefSeq:WP_010874588.1", "UniRef:UniRef100_P75540" ] }, "ips": { "uniref100_id": "UniRef100_P75540", "uniref90_id": "UniRef90_P75540" }, "psc": { "uniref90_id": "UniRef90_P75540", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_P75540", "cog_id": "COG0359", "cog_category": "J", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P75540", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75540" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "CNOCLNGIAL_532", "locus": "GCA000143945_00497" }, { "type": "cds", "sequence": "contig_1", "start": 279989, "stop": 280624, "strand": "+", "frame": 2, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P75539" ], "nt": "ATGGTGTCAAATAAGCTTGAACAGACCTTTAAGTTTGCCAACGACAAGAACATTGAACGTGCTGAGCGTGTTTTAATGCAAGCTTTAGTACAAGATGCCGTTGGTGTGGACTTAATCTTAACCAAGCTTGAACCGAAGGACTTCTTTGGTTTTCCGTTTAACTTTATCTTTCAAACAGCACAGGAAAACTATTCTGAGGGCAATAAGTTGTTTGGTTCGGCGCTTTTAGAGGCCGTTAAGTTTAAGCTGGATACCGATTCCAAAACACAAAGGGAAATTGAAGGTTTATTTGAGGACGTTTTACTCAAAGGTCTTCCCTTTTTGGAAAGGGACATCAAGGTCTTTGTTGATGTTGTCAAAAAAGCTTCGATTTTTCGCCAACTTAAACAGTTCGCCAAAAAGGTTGAAAAGGAAGAGTTTAAGGTCAAAGATGACCGTTTTGAGGGCTATTTACAAGCAATTCAAAACGACTTTACCAAGATTATTCACAGCGCCTTTGTTTCTATTCCCGGTTTGAGTTATGACGAAATTGGTCGTGAAGAGGAAGAGTTAATTCGCAAAGTGTACCGTGGTGAATTAACTGTAAAAGGGCTTAAAAGTGGTTTTTACCCTTTAGATCAATTAACCTCTGGTTGA", "aa": "MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIGREEEELIRKVYRGELTVKGLKSGFYPLDQLTSG", "aa_hexdigest": "f06d5a9e348bb88eb54d786ec7ffaa2e", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "3-prime", "pseudogene": { "start": 279989, "stop": 280927, "observations": { "mutation": [ 280622 ], "stop": [ 280622 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75539", "query_cov": 1.0, "subject_cov": 0.44608879492600423, "identity": 0.991, "score": 400.0, "evalue": 4.83e-137, "gene_start": 1, "gene_end": 211, "reference_start": 1, "reference_end": 211, "reference_sequence": "MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIVREEEELIPKVYRGELTVKGLKSGFYPLDQLTSGWKEGELIVVAARPGRGKTALLINFLQGAVNDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS" }, "paralog": false, "identity": 0.9903846153846154, "score": 597.0, "evalue": 1.61e-216, "description": "Internal stop codon at 280622. Nonsense mutation around 280622.", "psc": { "uniref90_id": "UniRef90_P75539", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75539", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "CNOCLNGIAL_533", "locus": "GCA000143945_00498" }, { "type": "cds", "sequence": "contig_1", "start": 280643, "stop": 281410, "strand": "+", "frame": 2, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P75539" ], "nt": "GTGGTAGCAGCGCGTCCAGGTCGCGGGAAAACAGCGCTTCTAATTAACTTTTTGCAGGGCGCGGTTAATGACAGCGAAAAGTTTGATAAAAACAAGGATGTGTTGCTGTTTTTTAGCTTAGAAATGCGTAACCGTGAAATTTATCAGCGCCATTTAATGCTAGAAAGCCAGGTAAATTACACACTCACGAGTCGTCAAAGGCTCGCTAATGTGTATGATGATTTAATTAAAGCATCAGAAGTATTGCGCGATCTTCCTATTAAAATTTTTGATTACAGCACGATTACGTTGGACGAAATTCGTGCTCAAATCACGGAAGTTTCAAAAACCAATAACGTTAAGTTGGTCGTAATTGACTATCTCCAACTAGTTAATGCATTTAAAAACAGCTATAACATTTCGCGTCAACAAGAGGTCACCATGATTTCCAAATCACTTAAAAGCTTTGCCAAGGAGTTTAACGTACCAATTATTGCCGCTGCACAGCTTTCTCGAAGAATAGAAGAACGCAAAGATTCGCGTCCCATTTTGTCGGATTTACGTGAATCGGGTTCGATTGAACAGGATGCTGACATGGTTTTATTCATCCACCGCGTCAAGGATGAGGACAGTGAAAATCAAGAAGCAATTGGTCACAACAATATTTTTCAAGTGGAGTTAATCTTGGAAAAAAACCGTAGTGGTCCGACTGGTAAAGTACAGTTTGACTTTCAAAGTGACGTTTCTACCTTTCGGGTTCGGGAATACAATCCTGATGGTTACAGTTAG", "aa": "MVAARPGRGKTALLINFLQGAVNDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS", "aa_hexdigest": "ecb9c031c3a78514b207c1530bb8a05d", "start_type": "GTG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 280343, "stop": 281410, "observations": { "mutation": [ 280622 ], "stop": [ 280622 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75539", "query_cov": 1.0, "subject_cov": 0.5391120507399577, "identity": 0.996, "score": 485.0, "evalue": 1.61e-169, "gene_start": 1, "gene_end": 255, "reference_start": 219, "reference_end": 473, "reference_sequence": "MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIVREEEELIPKVYRGELTVKGLKSGFYPLDQLTSGWKEGELIVVAARPGRGKTALLINFLQGAVNDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS" }, "paralog": false, "identity": 0.9915492957746479, "score": 677.0, "evalue": 2.07e-247, "description": "Internal stop codon at 280622. Nonsense mutation around 280622.", "psc": { "uniref90_id": "UniRef90_P75539", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75539", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "CNOCLNGIAL_534", "locus": "GCA000143945_00499" }, { "type": "cds", "sequence": "contig_1", "start": 281414, "stop": 281824, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTACAAAACAGTTAAATCGATCCCGCTTTTTTTGTTAGGCTCGATTGTGTTCACTGCTTGTTCAACACCACAATCAACCTTTCATTTACCTGTGCAAACGACCGTTAGCGCAATCAAAAAAGATATTAGTGGGAAAACAGCTACTGCTGTTAAGGCTGCTAGTAGTTCATCTAGTACTACTACATCTAACGATGACAACAACCAAAAGGGTTACTTCTTAGAAACTAATCGTTCCACCGGTACTTACGACCCTAACAATTCCACCCGGTTAATTAAATTAGGTGAAAGTGGTGATTTTCATGCAGCTGACCAGAATAAACCGGAAGAAGCCTTGTTTGAACGACTTTACGGCGGCATTGCTTCCTTATTAAATTTCCGCATTATTAAACCTGCTTTAACTTATTGA", "aa": "MKYKTVKSIPLFLLGSIVFTACSTPQSTFHLPVQTTVSAIKKDISGKTATAVKAASSSSSTTTSNDDNNQKGYFLETNRSTGTYDPNNSTRLIKLGESGDFHAADQNKPEEALFERLYGGIASLLNFRIIKPALTY", "aa_hexdigest": "3c9bf81ed1388f87208e723ee440fe3a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14759.450199999985, "isoelectric_point": 9.239302635192868 }, "id": "CNOCLNGIAL_535", "locus": "GCA000143945_00500" }, { "type": "cds", "sequence": "contig_1", "start": 281918, "stop": 282544, "strand": "+", "frame": 2, "gene": null, "product": "putative lipo MG095-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75538", "UniRef:UniRef90_P75538" ], "nt": "TTGGCTAATAACTTAATTGTCGCTGATGATGGCAACAACAACTTTTGGTTTGGTTTAAAGTCACTTAGCTTTAATGCAGCTAAGTTGACTGACAGCGCCCAAACACAAATGGCGCAAAAAACCACTCAAGCAGTTACCTTGAAAAGTCAAGCGCAAATGTCAAGTACAAATACCAAAAACACCAACAAAAAGATTGATTTGCGCGATAAGATTACTTTATCGAGTACGATGAATACTCAAGGAAGTGGTGATAACAAAAACCCATCTAGTGGTCTTATTCAAAAGTTAGTGAGTGTCGAAAATATTGAAGCTGAATTTAGTTTTGTTAAAACTGGCTTCAACGGTAATGAAATTAAATTTGGCGATTTTGTAACTGAGAATTCACCCACAACCACTCAACTTAAGCAAGTTTGGAAAAAGAAGTGGGGCACGGAATTGAAAAAGACAAACTATAAGCTTCAATTGAACAACTTTAGTCTATTGCTAACTTACACACCAGAAGTTAATAAAGTTGAAAAAGGTAACAATGGTGATTCCAATAAAGGCACGATCGCTACGCCAAACGGTTTTAGCTTCTTGTACCCAGCTAATTTAAATGAGACACCAAGCAGTAGCAGTAGTTATTGA", "aa": "MANNLIVADDGNNNFWFGLKSLSFNAAKLTDSAQTQMAQKTTQAVTLKSQAQMSSTNTKNTNKKIDLRDKITLSSTMNTQGSGDNKNPSSGLIQKLVSVENIEAEFSFVKTGFNGNEIKFGDFVTENSPTTTQLKQVWKKKWGTELKKTNYKLQLNNFSLLLTYTPEVNKVEKGNNGDSNKGTIATPNGFSFLYPANLNETPSSSSSY", "aa_hexdigest": "997ab548c6713105ff3b405ba37f5068", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 281621, "stop": 282778, "observations": { "mutation": [ 281822, 282542 ], "stop": [ 281822, 282542 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75538", "query_cov": 1.0, "subject_cov": 0.4581497797356828, "identity": 0.986, "score": 393.0, "evalue": 2.49e-134, "gene_start": 1, "gene_end": 208, "reference_start": 169, "reference_end": 376, "reference_sequence": "MKYKTVKSIPLFLLGSIVFTACSTPQSTFHLPVQTTVSAIKKDISGKTATAVKAASSSSSTTTSNDDNNQKGYFLETNRSTGTYDPNNSTRLIKLGESGDFHAADQNKPEEALFERLYGGIASLLNFRIIKPALTYWNTVTPSLKAIGKSSNLITFSQDIDETELQRALANNLIVADDGNNNFWFGLKSLSFNSAKLTDNAQTQMAQKTTQAVTLKSQAQMSSTNTKNTNKKIDLRDKITLSSTMNTQGSGDNKNPSSGLIQKLVSVENIEAEFSFVKTGFNGNEIKFGDFVTENSPTTTQLKQVWKKKWGTELKKTNYKLQLNNFSLLLTYTPEVNKVEKGNNGDSNKGTIATPNGFSFLYPANLNETPSSSSSYWTNVTDLTKAATDTENTNLLNDLQKSQEQVNQFVAAITQNHLDVSEAALTKKQFGSLSISDFFKAIFKENGKDTKAKS" }, "paralog": false, "identity": 0.9896103896103896, "score": 749.0, "evalue": 5.44e-277, "description": "Internal stop codon at 281822, 282542. Nonsense mutation around 281822, 282542.", "psc": { "uniref90_id": "UniRef90_P75538", "product": "Uncharacterized lipoprotein MG095 homolog", "uniref50_id": "UniRef50_P75538", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75538", "UniRef:UniRef50_P75538", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75538", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75538" ], "product": "Uncharacterized lipoprotein MG095 homolog" } }, "id": "CNOCLNGIAL_536", "locus": "GCA000143945_00501" }, { "type": "cds", "sequence": "contig_1", "start": 282608, "stop": 282778, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAGAGTCAGGAACAAGTCAATCAATTTGTTGCTGCTATTACCCAAAATCACCTCGATGTTTCCGAAGCTGCTTTAACTAAAAAACAGTTTGGTAGCCTTAGCATTAGTGATTTCTTTAAAGCCATCTTTAAGGAAAATGGCAAAGACACTAAAGCTAAAAGTTAA", "aa": "MQKSQEQVNQFVAAITQNHLDVSEAALTKKQFGSLSISDFFKAIFKENGKDTKAKS", "aa_hexdigest": "2b4ec1a1cb8e74bb232977eaeff3cd23", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6233.969299999999, "isoelectric_point": 9.158652687072756 }, "id": "CNOCLNGIAL_537", "locus": "GCA000143945_00502" }, { "type": "cds", "sequence": "contig_1", "start": 283206, "stop": 284249, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_234", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75537", "UniRef:UniRef90_P75537" ], "nt": "TTGACTGGTGTACTTGAAACAATTCCCCAACTGCAAAAGGAAATAAAAGCTGCTAATGACATTTTCCCTGTCAATGAACAAAGTCAAATTCCCCGTGTAGAAGCACGCTTATTTGACTTTTTGGGCAGTGGCTTTTTCCCGGTATTGAACCCGAAAGGTTTAGGGTTAGCGGACAACGTTGCTAGTTTGTTTGAACAGTTTCAGCTAGCATCAATAAACTTAAGTGGTTTTGAAGTCAAACTGATTAAAACGCATGACATTGTCATTAAGAGTAAGGTACGTTACAGCTTTGAAGTGCAAATGCAGTTCCATGCTGTTTACCAAAATAACGACACTAAAGCTAATTTAGATTTTGCGCTCAATATTAGTACGATTAACTTCGCTACCTTACAAGAATTACAAAACAGTTTTGATCTTCAAGGTAGTGATTTAACGGCTGGGTTGTTTTACAAATACAGCGTTAATAAGTTAACTAGTGGTACTAACGATTTAACCACAATTGCTAAAACCGCTTTAGGTGAAAACATTATCCAAAAGCAAGTGAGCCTAACACAATCAATTATTAAGCCACGCCTAGAAGCTGCAAAAACTCAGTACAAACAGGATATTATTGCACCATTTGCAAAGGAACGTCAAGCTGCTTTGGCACAACATTTAAAAGAAATAGAAGAGGCCAAACAACGTGCCGAGCAATTATTAAAAGAACAGCAAGAAGCGGAAAAACGGCGCCAAGAAGAAGTTAAGAATGTCGCAGAAACACAACAGTTTAATGATAGCTTAACTTCAGCACAAAAATTTAAAGAGTACTGGTTAAAACAAGGTAAGGATGTTACCAAAAAGGTTGAACTCATTCAGGCGCTGAAATCATCTTTCTTCCGCAACCAAAACCGTACCTTTAACTTTTTAATTGCCGGTTTTCGAACTGCCATTGATTGGTACTACAATCAAGAGAAAAATAATACCACTGCTAAAAATAACGCTTTTGGTAAGAATGGCATTCAGTTCCCTGTGGCTGGCTTTCAGGGAATTTACATGTCACAGTGA", "aa": "MTGVLETIPQLQKEIKAANDIFPVNEQSQIPRVEARLFDFLGSGFFPVLNPKGLGLADNVASLFEQFQLASINLSGFEVKLIKTHDIVIKSKVRYSFEVQMQFHAVYQNNDTKANLDFALNISTINFATLQELQNSFDLQGSDLTAGLFYKYSVNKLTSGTNDLTTIAKTALGENIIQKQVSLTQSIIKPRLEAAKTQYKQDIIAPFAKERQAALAQHLKEIEEAKQRAEQLLKEQQEAEKRRQEEVKNVAETQQFNDSLTSAQKFKEYWLKQGKDVTKKVELIQALKSSFFRNQNRTFNFLIAGFRTAIDWYYNQEKNNTTAKNNAFGKNGIQFPVAGFQGIYMSQ", "aa_hexdigest": "9cf2a8e6f1af61ca881d13d8d0705a27", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 282906, "stop": 284552, "observations": { "mutation": [ 283095, 283194, 284247, 284439, 284481 ], "stop": [ 283095, 283194, 284247, 284439, 284481 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75537", "query_cov": 1.0, "subject_cov": 0.5481832543443917, "identity": 0.997, "score": 630.0, "evalue": 8.41e-223, "gene_start": 1, "gene_end": 347, "reference_start": 117, "reference_end": 463, "reference_sequence": "MKFPKKRWQKWAFLTGSFTVLPTLIAFFSILPHSSVLEPSHLRFNSTSQRYQSDNSHLLNLHGEAFSDADLNAVAEHTDWDANFALFQQKFKTKPITVNQLGLVDVFNLLSGWKQSLTGVLETIPQLQKEIKAANDIFPVNEQSQIPRVEARLFDFLGSGFFPVLNPKGLGLADNVASLFEQFQLASINLSGFEVKLIKTHDIVIKSKVRYSFEVQMQFHAVYQNNDTKANLDFALNISTINFATLQELQNSFDLQGSDLTAGLFYKYSVNKLTSGTNDLTTIAKTALGENIIQKQVSLTQSIIKPRLEAAKTQYKQDIIAPFAKERQAALAQHLKEIEEAKQRAEQLLKEQQEAEKRRQEEVKNVAETQQFNDSLTSAQKFKEYWLKQGKDVTKKVELIQALKSSFFRNQNRTFNFLIAGFRTAIDWYYNQEKNNTTAKNNAFGKNGIQFPVAGFQGIYMSQWLRDELSGKTDIKLNLKSLSVQNENKNSSINWNKQKRIEIKQVKPFNYSFEINLKYTGSYNVSLWYLIGAAIGGIPTSWSGTMDMKFIVDGDLDSGIVTKQDYPGSKFEFTEDKLWFTLHVKQQIKVKEQGFMNLLKGQSLDNLDLRTGTTKPPVVDLASYLHFVILTAK" }, "paralog": false, "identity": 0.9908759124087592, "score": 1021.0, "evalue": 0.0, "description": "Internal stop codon at 283095, 283194, 284247, 284439, 284481. Nonsense mutation around 283095, 283194, 284247, 284439, 284481.", "psc": { "uniref90_id": "UniRef90_P75537", "product": "Uncharacterized protein MPN_234", "uniref50_id": "UniRef50_P75537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75537", "UniRef:UniRef50_P75537" ] }, "pscc": { "uniref50_id": "UniRef50_P75537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75537" ], "product": "Uncharacterized protein MPN_234" } }, "id": "CNOCLNGIAL_538", "locus": "GCA000143945_00503" }, { "type": "cds", "sequence": "contig_1", "start": 284250, "stop": 284441, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "TTGCGTGACGAGCTCTCTGGTAAGACCGACATTAAGCTCAACTTAAAAAGTTTAAGTGTTCAGAATGAAAATAAAAATTCTTCCATTAATTGGAACAAGCAAAAAAGAATTGAGATTAAACAAGTAAAACCATTTAACTATTCTTTTGAAATTAACTTGAAGTACACTGGTAGCTATAATGTCAGTCTTTGA", "aa": "MRDELSGKTDIKLNLKSLSVQNENKNSSINWNKQKRIEIKQVKPFNYSFEINLKYTGSYNVSL", "aa_hexdigest": "b220fc0c1ec9eeda9cb2e3f0537a031c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 70, "aa_cov": 0.9841269841269841, "hmm_cov": 1.0, "evalue": 6.879011915252346e-19, "score": 63.76200866699219, "start": 1, "stop": 62 } ], "seq_stats": { "molecular_weight": 7395.322900000001, "isoelectric_point": 9.697672843933105 }, "id": "CNOCLNGIAL_539", "locus": "GCA000143945_00504" }, { "type": "cds", "sequence": "contig_1", "start": 284493, "stop": 284759, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGGATATGAAGTTTATCGTTGATGGTGATTTGGATAGCGGTATTGTTACCAAGCAAGATTATCCCGGTTCTAAGTTTGAATTTACCGAAGACAAACTGTGGTTTACCCTGCACGTTAAACAACAAATTAAAGTTAAAGAGCAGGGCTTTATGAACTTGCTTAAGGGTCAAAGTTTGGACAACTTGGATCTACGCACTGGTACAACTAAACCGCCAGTAGTTGATTTAGCGAGCTACCTCCACTTTGTTATTTTGACAGCTAAGTAG", "aa": "MDMKFIVDGDLDSGIVTKQDYPGSKFEFTEDKLWFTLHVKQQIKVKEQGFMNLLKGQSLDNLDLRTGTTKPPVVDLASYLHFVILTAK", "aa_hexdigest": "26088d073aaa33af3526bbdd31353688", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 48, "aa_cov": 0.5340909090909091, "hmm_cov": 1.0, "evalue": 5.011332287463903e-18, "score": 60.96890640258789, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 10034.49619999999, "isoelectric_point": 6.040245628356933 }, "id": "CNOCLNGIAL_540", "locus": "GCA000143945_00505" }, { "type": "cds", "sequence": "contig_1", "start": 284784, "stop": 285125, "strand": "+", "frame": 3, "gene": "ung", "product": "uracil-DNA glycosylase", "db_xrefs": [ "COG:COG0692", "COG:L", "EC:3.2.2.27", "GO:0004844", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0097510", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_P75536", "UniRef:UniRef90_P75536" ], "nt": "ATGGAACAACTTTTAGACCAGTTTTTAAGTGGTGTTTTAAGCGAATGGAAGGCTTTTATTTTGCAAGAAGCCAAACAGACCTATTTTCAAGAATTGTTAGCTAAACTAAAAAATGTCGAGCAAGAGCTAACTCCCAAAGCCTCTCAAGTCTTTCGTCCCTTTAGCTTTTTTGCACCAAATAACACTAAACTAATTATTTACGGTCAGGATCCATATCCTAATCCACAACACGCTTGTGGTCTTTCGTTTGCCTCTAACGCCCCTAAACTACCACAAAGCTTAAAGCGGATGATTCTGCGCTTGCAGCAAGAGTATCCGGAACTAGCAGGTCAAAATCACTGA", "aa": "MEQLLDQFLSGVLSEWKAFILQEAKQTYFQELLAKLKNVEQELTPKASQVFRPFSFFAPNNTKLIIYGQDPYPNPQHACGLSFASNAPKLPQSLKRMILRLQQEYPELAGQNH", "aa_hexdigest": "49a9ea9bbb4c4529cd72f5f243a6f61a", "start_type": "ATG", "rbs_motif": null, "genes": [ "ung" ], "truncated": "3-prime", "pseudogene": { "start": 284784, "stop": 285428, "observations": { "mutation": [ 285123, 285147, 285228 ], "stop": [ 285123, 285147, 285228 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75536", "query_cov": 1.0, "subject_cov": 0.4708333333333333, "identity": 1.0, "score": 226.0, "evalue": 1.96e-73, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MEQLLDQFLSGVLSEWKAFILQEAKQTYFQELLAKLKNVEQELTPKASQVFRPFSFFAPNNTKLIIYGQDPYPNPQHACGLSFASNAPKLPQSLKRMILRLQQEYPELAGQNHWTKQLLEGWAQQGILLLNGVFTTNAFQTNAHRNWGWEQFNCHLLDFLLSQKLYVLLVFLGKQTENFVLKKIGGASQFASLSYPHPSPLTGRKFFDHPDALFKQINQWLKHHNHTPIDWTNGQVQYQF" }, "paralog": false, "identity": 0.985981308411215, "score": 425.0, "evalue": 4.13e-154, "description": "Internal stop codon at 285123, 285147, 285228. Nonsense mutation around 285123, 285147, 285228.", "psc": { "uniref90_id": "UniRef90_P75536", "gene": "ung", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_P75536", "cog_id": "COG0692", "cog_category": "L", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0097510" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75536", "EC:3.2.2.27", "UniRef:UniRef50_P75536", "COG:COG0692", "COG:L", "KEGG:K03648", "GO:0004844", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0097510" ] }, "pscc": { "uniref50_id": "UniRef50_P75536", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75536" ], "product": "Uracil-DNA glycosylase" } }, "id": "CNOCLNGIAL_541", "locus": "GCA000143945_00506" }, { "type": "cds", "sequence": "contig_1", "start": 285704, "stop": 286669, "strand": "+", "frame": 2, "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "db_xrefs": [ "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681", "SO:0001217", "UniRef:UniRef50_P47344", "UniRef:UniRef90_P75535" ], "nt": "ATGGATAAATTAATAAATAAACCTCAACCATTAACTTCTGATATTTCCACTTCTGGATTTATTTACTTTGCGGTTGTTTTTGTAGCCATTATGGGCTACCTTTTATTCAAAAATCTACTGTTTTTATTTTTCTTTAAACGCTATCCAAAAAACACTACCAAAATTGGGGTAGGTAACATTACCACGATTGCCATAATCATTGCTGTGGCGGTGTCGATTGTTTTAGTGTTAATGGCACTAGCAGGCGGTTTAGCCGCTGCTTTGTTCCGTGGTTATCCTGGTTTTCGGGTCACCTTGGAGTTAATCCTTGTAAAGATTTCGGGACTGTTGTTTGGTCCAATTGTCGGCATTTTTTCCGCGGCCACAATTGACTTTTTAACGGTCATCTTTTCGGGTGGGGTTTTTAACGTTGGTTATGTTTTAGGTGCCATCTTAACCGGGATGATTGCTGGCATACTGCGTGAAGTTTTAATTTCAACAGCACTTTTACACAACCGTAATTTATCGGATTTTGCCTACCTAGTGCTCTCGATTGGCATGGTCATTGCCGCCTTTTTAATTACCCAGTTCTTTGTGCTTGGGATAAGTAATAACCTTAAGGAGATTAAGGGTGATGAAGAGTTTCGCTTGAAATTTAACGCACCACCAATTGTCTTTGAGCTTTCGTTAACCCAGTACGCCAACATTCTGCTCTACTTTACCATTGCCATAGTTATTGCTATGCTGGTGTTGTACATTGTTTGGTTAGTCAAACAGCGTCACTTAAGTTTTGAGCACTCTCGTTTCTTTTACCGCAGTTACAAACATGCTAATCACCAGTTTACCCTGTTTGTACTAACTAAGGAAAACTGGTTCTATTTAATCTTGAATGTAATCACCTTAGCTTCCACTAGTTTGTTGATGATCAACATTGCCTTCATTCCGATCTTTGACACGCAAACAACTGGTCAAACATACGAGTTTTGA", "aa": "MDKLINKPQPLTSDISTSGFIYFAVVFVAIMGYLLFKNLLFLFFFKRYPKNTTKIGVGNITTIAIIIAVAVSIVLVLMALAGGLAAALFRGYPGFRVTLELILVKISGLLFGPIVGIFSAATIDFLTVIFSGGVFNVGYVLGAILTGMIAGILREVLISTALLHNRNLSDFAYLVLSIGMVIAAFLITQFFVLGISNNLKEIKGDEEFRLKFNAPPIVFELSLTQYANILLYFTIAIVIAMLVLYIVWLVKQRHLSFEHSRFFYRSYKHANHQFTLFVLTKENWFYLILNVITLASTSLLMINIAFIPIFDTQTTGQTYEF", "aa_hexdigest": "a7dbac4392ccd8238898be0cc2fb2e1c", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatC" ], "truncated": "3-prime", "pseudogene": { "start": 285704, "stop": 286972, "observations": { "mutation": [ 286667 ], "stop": [ 286667 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75535", "query_cov": 1.0, "subject_cov": 0.6701461377870563, "identity": 0.96, "score": 561.0, "evalue": 2.22e-198, "gene_start": 1, "gene_end": 321, "reference_start": 1, "reference_end": 321, "reference_sequence": "MDKLINKPQPLTSDISTSGFIYFAVVFVAIMGYLLFKNLLFLFFFKRYPKNTPKIGVGNITTIAMIIAVAVSIVLVLMALAGGLAAALFRGYPGFRVTLELILVKISGLLFGPIVGIFSAATIDFLTVIFSGGVFNVGYVLGAILTGMIAGILREVLISTALLHNRNLSDFAYLVLSIGMVIAAFLITQFFVLGISNNLKEIKGDEEFRLKFNAPSIVFELSLTQYANILLYFTIAIVIAMLVLYIVWLVKQRHLSFEHSRFFYRSYKHANHQFTLFVLTKENWFYLILNVITLASTSLLMINIAFIPIFDTQTTGQTYEFWLLARLLFAPVIFLLDIIVIYPILLLLTPLMLKGFKTAVSKNQRKTLKQSFTDLQSVVLPIINKRKHQQLRQEELKRLARATHFDLTEGEMEKLLVEFKTITQSFDRVMNIDTTSVEPMYAPFNTSPTPLRKDKVIVEKHPEKLLANCKEMSVGFVKV" }, "paralog": false, "identity": 0.966824644549763, "score": 779.0, "evalue": 1.59e-286, "description": "Internal stop codon at 286667. Nonsense mutation around 286667.", "psc": { "uniref90_id": "UniRef90_P75535", "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "uniref50_id": "UniRef50_P47344", "cog_id": "COG0721", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75535", "UniRef:UniRef50_P47344", "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P47344", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47344" ], "product": "Uncharacterized protein MG098" } }, "id": "CNOCLNGIAL_542", "locus": "GCA000143945_00507" }, { "type": "cds", "sequence": "contig_1", "start": 286706, "stop": 287143, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGACATTATTGTGATCTATCCAATTCTGCTGTTATTAACCCCGCTAATGCTCAAAGGTTTTAAAACAGCAGTTTCTAAAAACCAACGCAAAACACTAAAACAAAGCTTTACTGATTTGCAAAGTGTAGTGTTGCCCATTATTAACAAACGTAAACACCAGCAGTTGCGCCAAGAAGAGTTAAAACGCTTAGCACGAGCCACTCACTTTGATTTGACAGAAGGCGAAATGGAAAAGCTCTTGGTCGAATTTAAAACTATTACCCAGAGCTTTGACCGGGTGATGAACATTGATACCACCAGTGTGGAACCAATGTACGCACCTTTCAATACATCACCAACGCCACTGCGTAAAGATAAGGTAATAGTGGAAAAGCATCCCGAAAAATTATTAGCCAACTGTAAGGAAATGAGTGTTGGCTTTGTCAAAGTATAG", "aa": "MLDIIVIYPILLLLTPLMLKGFKTAVSKNQRKTLKQSFTDLQSVVLPIINKRKHQQLRQEELKRLARATHFDLTEGEMEKLLVEFKTITQSFDRVMNIDTTSVEPMYAPFNTSPTPLRKDKVIVEKHPEKLLANCKEMSVGFVKV", "aa_hexdigest": "b81d3988cf6cbfa4502208105427e717", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16753.69129999999, "isoelectric_point": 9.605740928649901 }, "id": "CNOCLNGIAL_543", "locus": "GCA000143945_00508" }, { "type": "cds", "sequence": "contig_1", "start": 287398, "stop": 287547, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "TTGGAAGACTATATCCCGCCGTTTTCAGCAACTGTGTTTGAATTGCTAAACAACAGTGGCGCTTTATTAGTTGGTAAAGCGAACCTCGACGAGTTTGGTTTAGGCGGTACGGGGCTCCATTCGGGTTTTGGTTTTGTCCACCACCCTTGA", "aa": "MEDYIPPFSATVFELLNNSGALLVGKANLDEFGLGGTGLHSGFGFVHHP", "aa_hexdigest": "1f21a5e9cf2fece19ab7d8f96b820894", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 48, "aa_cov": 0.9795918367346939, "hmm_cov": 1.0, "evalue": 7.184402801890184e-13, "score": 43.77743148803711, "start": 2, "stop": 49 } ], "seq_stats": { "molecular_weight": 5174.751499999999, "isoelectric_point": 4.834747505187988 }, "id": "CNOCLNGIAL_544", "locus": "GCA000143945_00509" }, { "type": "cds", "sequence": "contig_1", "start": 287596, "stop": 287955, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "GTGGCGCGCGGCATTGTTCCCTTTTCAATTGGTACGGACACGGGAGATTCGGTACGCCGTCCAGCTAGCATTTGTAACATTGTTGGTTTTAAGCCCACCTATGGTTTGATATCGCGGAACGGAGTGTATCCTTACGCGCCTAGTTTAGACCATGTCGGGATCTTTGCGCGGTATGTGTATGATGTAGCACTTGTTAGTGATGAAATCATTAAACATGACAAAGCTGATTTTTCCGCTCAAAAATCTCCTGATGCTGGCAAGTTTACCAGGTCCTTAAAAGAGTCCTTCAACAAGCAAATCAAGATAGGTTATCTAAAACCGCTAGAGGAATGGTTTGACATTGAACTGAGCAAAAAGTGA", "aa": "MARGIVPFSIGTDTGDSVRRPASICNIVGFKPTYGLISRNGVYPYAPSLDHVGIFARYVYDVALVSDEIIKHDKADFSAQKSPDAGKFTRSLKESFNKQIKIGYLKPLEEWFDIELSKK", "aa_hexdigest": "d40eabf11527c5f792c7ba569a20d339", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 113, "aa_cov": 0.9495798319327731, "hmm_cov": 0.9911504424778761, "evalue": 6.052213194850615e-41, "score": 136.30691528320312, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 13303.072799999987, "isoelectric_point": 8.989681053161618 }, "id": "CNOCLNGIAL_545", "locus": "GCA000143945_00510" }, { "type": "cds", "sequence": "contig_1", "start": 287980, "stop": 288459, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "TTGGAGGGTTGTGAGTTAATTCCGTTTCACTTTCCGCTTGAACTGCTAGAGGTAATTGATCCAGTTTACAAGCTGATTAGTTACAGTGAAGCGGTGAGCTGTTACAGTAATCTCACTGGGATTGTCTTTGGTCAAAAATTGTTTGAACCCAACCAAGCGAGTGACTTTTCCAAAACAATTACTGCCAACCGCGACCGTTTCTTTGGTGAGCAGTTAAAACGCCGCTTTATTATTGGTGCTTTTGGTACTGACAAGAATAACTTTACCAAGTACTTTGAAAAGGCTCAAAAGATCCGCCGGGTAATGGTTGATGCTTACTTAAACCTGTTTAAAGAAGCGGATTTCATTGTGTCACCTAGTGCATCCGGCTTTACTAAAACAATAGCTGCGGTGCAAAAGGGTGAGAGCTTTACTAACCTCGTGGATGACTTTTTACAACTAGCTAACTTTGCTGGTAATCCTTCAATTACTATTCCCTGA", "aa": "MEGCELIPFHFPLELLEVIDPVYKLISYSEAVSCYSNLTGIVFGQKLFEPNQASDFSKTITANRDRFFGEQLKRRFIIGAFGTDKNNFTKYFEKAQKIRRVMVDAYLNLFKEADFIVSPSASGFTKTIAAVQKGESFTNLVDDFLQLANFAGNPSITIP", "aa_hexdigest": "dc2a056738c208e9952c47c45cafb867", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 162, "aa_cov": 0.9874213836477987, "hmm_cov": 0.9382716049382716, "evalue": 8.373202035005293e-27, "score": 89.70026397705078, "start": 3, "stop": 159 } ], "seq_stats": { "molecular_weight": 17931.30759999999, "isoelectric_point": 5.8598955154418935 }, "id": "CNOCLNGIAL_546", "locus": "GCA000143945_00511" }, { "type": "cds", "sequence": "contig_1", "start": 288575, "stop": 290008, "strand": "+", "frame": 2, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.-", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681", "KEGG:K02434", "RefSeq:WP_014574916.1", "SO:0001217", "UniParc:UPI000012B157", "UniRef:UniRef100_P75533", "UniRef:UniRef50_P75533", "UniRef:UniRef90_P75533" ], "nt": "ATGATTAACTTTGAAGCAATTATTGGGATTGAGGTACACGTGGTCTTAAACACTGCTACCAAAATGTTTTCCCCAGCGCCCAACCAAGCGCAAAACGCCACACCAAACCAGTTCATAAACGCGATTGATTTGGGCTTGCCTGGTACCATGCCCCAGGTCAATGAGCAGGCGGTACAAAAGGCGTTAATCCTTGCTGACGCGCTCAACATGCAACGGGTACAGCCAGTCTTAGTGTTTGACCGTAAGCACTACTTTTACCAAGATCTACCTAAGGGTTTTCAGATTACCCAGCAAAACTTTCCGATTGCGCAAAACGGTTACGTTGAAATTGTGGAAAACAACAAAGCGCAGCGGATTATCATTGAACGGTTTCACCTAGAGGAAGATACTGCCAAGCAACATTTTGTCGATGGGCAAATTCTGCTCGACTTTAACCGTTGTGGTGCACCGCTAATTGAAGTAGTTACCGCGCCCGTGATTAAGAGTGCTAAGCAGTCTAAGGCATACTTGCAAAAGCTACGTCAAATTTTGATTGTTAACAACATTTCCAACGCTAAGCTCGAAGATGGCTCGATGCGCAGTGACTGTAATGTATCAGTGCGCTTAAAGGGACAAACTGCTTTTGGCACCAAGGTCGAAATTAAGAACATTAACTCACTCAATAATGTGGAAAAGGCAATTGATTTGGAAATAGCACGTCAAGTCAAAGCACTAATTAAAGGCGAACCGGTTCAACAAGTTACCCTAACTTATGACGACAAAACCAATACCAATGTCTTCATGCGTAAAAAGGACAATTCAGTCGATTACCGTTATTTCATTGAACCCAACATTATGAGCAGTAACATTGATGAACTGCTACAAAAACCCAACAAGGCCTTTAACTTAACTGAGTTCTTTACCAAGCTCAAGGAAGCTGGGGTTAATGAGCAGCTAAATCAATTAGTAGTCGATGATCTGACTCTTTTTAATGCTTACACCAAGATTAACAGTGTCATTAACAGTCCCCAAGATACCATTCGCTGGTTGTGCATTGAGTTAATGGGACAACTCAACAAGCTACAAAAACCGCTCAAGGACAAAACGATTGATCACTTAATTATCCTAATCCAAATGGTACAGAAGGGCACCGTGAACCAAAAACAAGCAAAGCAGTTAATTGAGTTAATGTTAGACAATGGGCAAGACCCCCAATCGCTGGCTAAACTACACAACTTAGAACAGATAACTGATGAAAAACAACTAACGCAAATCATCCAACAAATCTTTAAGGAAAACGAGGGTGAAATCCTTAAGAACTTAGATCGAGTTGAACGAATTCAAAAGCTAATTATAGGTCAAGTTATGCAAAGAACCCACAATCGCGCCAATCCCCAGCAAGTTTTTATAATTGTTGAAAAGTTGCTTCATGATTTCAGCGAAAGAGCAAGCTAA", "aa": "MINFEAIIGIEVHVVLNTATKMFSPAPNQAQNATPNQFINAIDLGLPGTMPQVNEQAVQKALILADALNMQRVQPVLVFDRKHYFYQDLPKGFQITQQNFPIAQNGYVEIVENNKAQRIIIERFHLEEDTAKQHFVDGQILLDFNRCGAPLIEVVTAPVIKSAKQSKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQTAFGTKVEIKNINSLNNVEKAIDLEIARQVKALIKGEPVQQVTLTYDDKTNTNVFMRKKDNSVDYRYFIEPNIMSSNIDELLQKPNKAFNLTEFFTKLKEAGVNEQLNQLVVDDLTLFNAYTKINSVINSPQDTIRWLCIELMGQLNKLQKPLKDKTIDHLIILIQMVQKGTVNQKQAKQLIELMLDNGQDPQSLAKLHNLEQITDEKQLTQIIQQIFKENEGEILKNLDRVERIQKLIIGQVMQRTHNRANPQQVFIIVEKLLHDFSERAS", "aa_hexdigest": "4e03be2185b8f4886ffdaf587fc56391", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B157", "ncbi_nrp_id": "WP_014574916.1", "uniref100_id": "UniRef100_P75533", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B157", "RefSeq:WP_014574916.1", "UniRef:UniRef100_P75533" ] }, "ips": { "uniref100_id": "UniRef100_P75533", "uniref90_id": "UniRef90_P75533" }, "psc": { "uniref90_id": "UniRef90_P75533", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.-" ], "uniref50_id": "UniRef50_P75533", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P75533", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75533" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" }, "genes": [ "gatB" ], "id": "CNOCLNGIAL_547", "locus": "GCA000143945_00512" }, { "type": "cds", "sequence": "contig_1", "start": 289983, "stop": 290264, "strand": "+", "frame": 3, "gene": "mngR", "product": "putative HTH-type transcriptional regulator MG101-like protein", "db_xrefs": [ "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700", "SO:0001217", "UniRef:UniRef50_P75532", "UniRef:UniRef90_P75532" ], "nt": "ATGATTTCAGCGAAAGAGCAAGCTAAAAAAGGTCAAATAATTTACCGTTACATTCTCCTTAAAATTCAAAGCTTTAAATGGCCTGCTGGCACCCGCATCTTCTCTGAACGCCAGCTCGAAATTCGTTTCGCTTCATCTAGAAGTCTCATTCGTACTATCCTCAATACCCTCTTAGGTAAAGATCTAATTCGCCACACTAAAGGTAATGCTGGTTACTTTGTCGCTAAGAATGCTGGTTTTAGTTTCTTTCATAAAACCCAGGACAACAAATTGCCCAAGTGA", "aa": "MISAKEQAKKGQIIYRYILLKIQSFKWPAGTRIFSERQLEIRFASSRSLIRTILNTLLGKDLIRHTKGNAGYFVAKNAGFSFFHKTQDNKLPK", "aa_hexdigest": "f903fecc7b0700eb7a22266261b6d67a", "start_type": "ATG", "rbs_motif": null, "genes": [ "mngR" ], "truncated": "3-prime", "pseudogene": { "start": 289983, "stop": 290567, "observations": { "mutation": [ 290262 ], "stop": [ 290262 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75532", "query_cov": 1.0, "subject_cov": 0.4189189189189189, "identity": 1.0, "score": 187.0, "evalue": 2.17e-58, "gene_start": 1, "gene_end": 93, "reference_start": 1, "reference_end": 93, "reference_sequence": "MISAKEQAKKGQIIYRYILLKIQSFKWPAGTRIFSERQLEIRFASSRSLIRTILNTLLGKDLIRHTKGNAGYFVAKNAGFSFFHKTQDNKLPKWAKLSTLMKNHLREVDRDVFSSIDSGVDFGNFKGLEAKLFDENKKNFLNLSFFGKDDVLQIFSEQNLQEQFFKDFAYNGIVVERKSSLICVDDETKNLLIYDLFYDDNNKFIVAMRSEYLNPRIKIINA" }, "paralog": false, "identity": 0.9948453608247423, "score": 381.0, "evalue": 3.22e-137, "description": "Internal stop codon at 290262. Nonsense mutation around 290262.", "psc": { "uniref90_id": "UniRef90_P75532", "gene": "MngR", "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog", "uniref50_id": "UniRef50_P75532", "cog_id": "COG2188", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003700" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75532", "UniRef:UniRef50_P75532", "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700" ] }, "pscc": { "uniref50_id": "UniRef50_P75532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75532" ], "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog" } }, "id": "CNOCLNGIAL_548", "locus": "GCA000143945_00513" }, { "type": "cds", "sequence": "contig_1", "start": 290283, "stop": 290651, "strand": "+", "frame": 3, "gene": "mngR", "product": "putative HTH-type transcriptional regulator MG101-like protein", "db_xrefs": [ "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700", "SO:0001217", "UniRef:UniRef50_P75532", "UniRef:UniRef90_P75532" ], "nt": "ATGAAGAACCACCTACGCGAAGTTGACCGTGATGTTTTCAGCTCAATTGACAGTGGCGTTGACTTTGGTAACTTTAAGGGTTTGGAAGCCAAGTTATTTGATGAAAACAAGAAGAATTTTTTAAACCTTAGCTTCTTTGGCAAGGATGATGTGCTCCAAATTTTTAGCGAGCAAAACCTCCAGGAACAATTCTTTAAGGACTTTGCTTACAACGGCATTGTGGTAGAGCGCAAAAGCTCCTTAATCTGTGTGGATGATGAGACTAAGAACTTACTGATCTACGACCTCTTTTATGATGACAACAATAAGTTCATAGTAGCAATGCGCTCTGAATATTTAAACCCCCGCATCAAAATTATTAATGCTTAA", "aa": "MKNHLREVDRDVFSSIDSGVDFGNFKGLEAKLFDENKKNFLNLSFFGKDDVLQIFSEQNLQEQFFKDFAYNGIVVERKSSLICVDDETKNLLIYDLFYDDNNKFIVAMRSEYLNPRIKIINA", "aa_hexdigest": "4af42ae1fda77222ae6c7c66b9bf9861", "start_type": "ATG", "rbs_motif": null, "genes": [ "mngR" ], "truncated": "5-prime", "pseudogene": { "start": 289983, "stop": 290651, "observations": { "mutation": [ 290262 ], "stop": [ 290262 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75532", "query_cov": 1.0, "subject_cov": 0.5495495495495496, "identity": 1.0, "score": 246.0, "evalue": 2.77e-81, "gene_start": 1, "gene_end": 122, "reference_start": 101, "reference_end": 222, "reference_sequence": "MISAKEQAKKGQIIYRYILLKIQSFKWPAGTRIFSERQLEIRFASSRSLIRTILNTLLGKDLIRHTKGNAGYFVAKNAGFSFFHKTQDNKLPKWAKLSTLMKNHLREVDRDVFSSIDSGVDFGNFKGLEAKLFDENKKNFLNLSFFGKDDVLQIFSEQNLQEQFFKDFAYNGIVVERKSSLICVDDETKNLLIYDLFYDDNNKFIVAMRSEYLNPRIKIINA" }, "paralog": false, "identity": 0.9954954954954955, "score": 437.0, "evalue": 8.26e-159, "description": "Internal stop codon at 290262. Nonsense mutation around 290262.", "psc": { "uniref90_id": "UniRef90_P75532", "gene": "MngR", "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog", "uniref50_id": "UniRef50_P75532", "cog_id": "COG2188", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003700" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75532", "UniRef:UniRef50_P75532", "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700" ] }, "pscc": { "uniref50_id": "UniRef50_P75532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75532" ], "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog" } }, "id": "CNOCLNGIAL_549", "locus": "GCA000143945_00514" }, { "type": "cds", "sequence": "contig_1", "start": 290644, "stop": 291591, "strand": "+", "frame": 1, "gene": "trxB", "product": "thioredoxin-disulfide reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "EC:1.8.1.9", "GO:0004791", "GO:0005737", "GO:0019430", "GO:0071949", "RefSeq:WP_014325415.1", "SO:0001217", "UniParc:UPI0001DD64F9", "UniRef:UniRef100_A0AAV5N8K6", "UniRef:UniRef50_P47348", "UniRef:UniRef90_P75531" ], "nt": "ATGCTTAAAGTAAAGTCAGATTTTTTAACTAAAGATCAGGTTATTTACGATGTAGCGATAGTGGGTGCTGGGCCAGCTGGCATAGCAGCGGGTATTTATGGCAAACGCGCTAACCTCAATCTAGCCATTATTGAAGGTAGTACTCCCGGCGGTAAGGTTGTCAAAACCAACATTGTCGAGAACTATCCGGGTTACAAGTCAATTACCGGACCTGACTTGGGGTTGGAAATGTACAACCACCTAATTGATTTAGAGCCCACCTTTTTCTATGCTAACCTAATTAAGTTAGATAAGGCAGCAGATACCTTTATCTTGTACCTCGATGACAAAACTGTGGTGTTTGCCAAAACCGTTATTTACGCTACGGGAATGTTGGAGCGTAAACTGGGCGTAGCCAAAGAAGACCACTTTTACGGTAAGGGAATTAGTTACTGTGCCATTTGTGATGGGTCCTTGTACAAAGACCAGGTGGTGGGTGTTGTTGGTGGGGGGAACTCTGCCATTCAGGAAGCGTTGTATTTAGCTGGCATGGCTAAAACGGTGCACTTAATCCACCGCCGTGAGGGCTTTCGTGCTGATGAAACGGCGTTAAATAAACTGAGAAACTTACCGAATGTTGTTTTTCACTTGAACTATACCGTAAAAGAATTGTTGGGTAACAATACCCTAAACGGCATAGTGCTTCAAAACACGCTCGATCATTCAACAAAACAGATAGATCTCAATTGTGTCTTTCCCTATATTGGTTTTGAAAGTATTACCAAACCGGTGGAACACCTCAACTTAAAGTTAGATCCCCAGGGTTTTTTAATTACCAATGAACAGATGGAAACAAGTTTAAAGGGTCTGTTTGCAGCAGGTGATTGTCGTTCTAAGCACTTCCGTCAAATTGGTACTGCCATTAATGACGGCATTATCGCGGTGTTAACGATCCGTGATGTCCTTTAG", "aa": "MLKVKSDFLTKDQVIYDVAIVGAGPAGIAAGIYGKRANLNLAIIEGSTPGGKVVKTNIVENYPGYKSITGPDLGLEMYNHLIDLEPTFFYANLIKLDKAADTFILYLDDKTVVFAKTVIYATGMLERKLGVAKEDHFYGKGISYCAICDGSLYKDQVVGVVGGGNSAIQEALYLAGMAKTVHLIHRREGFRADETALNKLRNLPNVVFHLNYTVKELLGNNTLNGIVLQNTLDHSTKQIDLNCVFPYIGFESITKPVEHLNLKLDPQGFLITNEQMETSLKGLFAAGDCRSKHFRQIGTAINDGIIAVLTIRDVL", "aa_hexdigest": "cd6e1a03f59190c61bc4a710042cc1c1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64F9", "ncbi_nrp_id": "WP_014325415.1", "uniref100_id": "UniRef100_A0AAV5N8K6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64F9", "RefSeq:WP_014325415.1", "UniRef:UniRef100_A0AAV5N8K6" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N8K6", "uniref90_id": "UniRef90_P75531" }, "psc": { "uniref90_id": "UniRef90_P75531", "gene": "trxB", "product": "thioredoxin-disulfide reductase", "ec_ids": [ "1.8.1.9" ], "uniref50_id": "UniRef50_P47348", "cog_id": "COG0492", "cog_category": "O", "go_ids": [ "GO:0004791", "GO:0005737", "GO:0019430", "GO:0071949" ] }, "pscc": { "uniref50_id": "UniRef50_P47348", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47348" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "CNOCLNGIAL_550", "locus": "GCA000143945_00515" }, { "type": "cds", "sequence": "contig_1", "start": 291737, "stop": 292423, "strand": "+", "frame": 2, "gene": "whiA", "product": "putative cell division protein WhiA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47349" ], "nt": "TTGTTTAGCAATGCACTCCAGTTTAGTTTTCAAATCCACGAAACGAGCACTCACTGTGAGTTTAGTTTTAGGGCTTCGCAAACAGAGGTGGATCAGTTGTTAGCCTTTGATGCAACTCAGAGCGATTTACCGCTACAGAAGGCCTATGTCATTGGTGCCTTTTTGAGTGGTGGTAGTGTGAGCGATTTACTCCATTCCAGTAACTTTCACCTCCAAATTAGCACTAACAACGAACTGCAAATAAAACAGTTAATGAAGCTGTTTCATCCTTTTAAACAAACCACTAAGCGCCACCAGCTTTTGGTCTATGTTAAAAGCTATCAGGCCATTTGTGACTTCTTTAAACTAGTCGAAGCTTTTGATGGTTATCTAGCCTTTGAAGAAAACCAATTGCAAAAAAATTTTGCCTTAGAACAAGTGCGTAAAAGTAACTTAGAAATTGCTAACCTAATGCGCACTTTAAAGACCAGCACAAGCACAGCGGAACAGCTAAAAATCCTCATAAACAGTGCTGACTTTAAAAAGCAATCGCTCAACTTTCAGCGCTTTTGTTTAGTCAAACTAGACCATCCTGACTGGTCATTAGAACAAATTGCCCAGTTTTTTGAAAGGAAATACAAAGTGCAAATTACCCGTTCGGGAATTCAACACCTCAACGCTAAGTTAAAAAAACTAAACCAAAATTAA", "aa": "MFSNALQFSFQIHETSTHCEFSFRASQTEVDQLLAFDATQSDLPLQKAYVIGAFLSGGSVSDLLHSSNFHLQISTNNELQIKQLMKLFHPFKQTTKRHQLLVYVKSYQAICDFFKLVEAFDGYLAFEENQLQKNFALEQVRKSNLEIANLMRTLKTSTSTAEQLKILINSADFKKQSLNFQRFCLVKLDHPDWSLEQIAQFFERKYKVQITRSGIQHLNAKLKKLNQN", "aa_hexdigest": "d398808f0140b4e45111776700f91edf", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47349", "query_cov": 0.9868421052631579, "subject_cov": 0.8035714285714286, "identity": 0.569, "score": 258.0, "evalue": 3.35e-83, "valid": false, "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_P47349", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_P47349", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47349" ], "product": "Probable cell division protein WhiA" }, "genes": [ "whiA" ], "id": "CNOCLNGIAL_551", "locus": "GCA000143945_00516" }, { "type": "cds", "sequence": "contig_1", "start": 292698, "stop": 292859, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTATTAACTGATTTACAAAAGCGAATCTTCGCGATTGTGAAAAAAGAGAACGGCAAACCTATTCCTCCGGGAATAGTTGTCAGGATGATGGAGAATCAAGCTGGTTTTCCGGGAAAGCAACAGGTATACAGAGCAATTGATGACTTGTTAGAATGA", "aa": "MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLE", "aa_hexdigest": "29b02c844d00215c9ae3be8493903c03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6025.118200000003, "isoelectric_point": 9.599229621887208 }, "id": "CNOCLNGIAL_552", "locus": "GCA000143945_00517" }, { "type": "cds", "sequence": "contig_1", "start": 292929, "stop": 293876, "strand": "+", "frame": 3, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_P47350", "UniRef:UniRef90_P75529" ], "nt": "TTGGATCAAAAGTTTCAGGGCATTCTCAATTTAGGTAACAAGAACACTGGTTTTGTCCGTCCCTTAGATGATGACAAAACGGTGTACTATATCCATTTTTCCAACCTAGCGGGAGCACTGGATGGTGATCTAGTTGAATTCTGTCCGCTCGACAAACCACAGGTAGGTGATAAGTTTGATGCTGCAGTGCTCAAAATTGTGAAGCGCTCCCGGGTGCTGTACGCTGGTAACTTTTTGATTGAGTACTCCGATTTTGGTCAGGAGTTTCGCATTGTTGCTGACAATCCTCGTTTTTACCTCACCCCGATTGTCAATAAGGCCTCAGTTCCAGCTGAACTGGAGAGCAATACTAAGGTCGCTTTTCAAATTGATGAGTATGATCCTGCAAATAACCTGTGTAAAGTCTCCATTCAGCAAATCTTGGGGAATAACGATGAACCGTTAATTAACCTCAAGGCGATTATGTTAGACCACTCGATTGTCTTTGAAAAGAATGATGTGGTCGAACAACAAGCAGCGAAGTTACAGTTTGATGAAAAAGAGCAGAGTAAGCCCTACCGCAAGGACTTAACCGAACTTGCCTTTGTCACGATTGACCCCGCCACTTCCAAGGACTTGGATGATGCGATTTACGTCAAGCGCACCGACAAAGGTTTTGTTTTGTATGTTGCCATTGCTGATGTGGCTTACTATGTGCAGCGCAACAGTGAACTTGACATTGAAGCCCGTCATAAAACAAGCTCAATTTACCTCCCGGGATACTATGTGGTACCGATGTTACCAGAACGTTTATCAAACGAGCTGTGTTCTTTAAACCCAAATGAAAAGCGGTATGTGGTGGTGTGTGAGTTGAACTTTGATCATGAAGCACGCTTAAACTTTAGTGAAGTGTATCCCGCTACCATTGTCTCCCAACGTCGCTTTGCTTACAGTGAAGTTAATGATTGA", "aa": "MDQKFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDLVEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYSDFGQEFRIVADNPRFYLTPIVNKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFEKNDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVAIADVAYYVQRNSELDIEARHKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCELNFDHEARLNFSEVYPATIVSQRRFAYSEVND", "aa_hexdigest": "ed2d343e9e2f9c6656a2befbd9af0dfb", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "both", "pseudogene": { "start": 292698, "stop": 294179, "observations": { "mutation": [ 292857, 293874, 294123 ], "stop": [ 292857, 293874, 294123 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75529", "query_cov": 1.0, "subject_cov": 0.43388429752066116, "identity": 0.9940000000000001, "score": 621.0, "evalue": 1.65e-218, "gene_start": 1, "gene_end": 315, "reference_start": 78, "reference_end": 392, "reference_sequence": "MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLEWHIFRKSGGATNQLLINYELADPVLDQKFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDLVEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYSDFGQEFRIVADNPRFYLTPIVNKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFEDNDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVAIADVAYYVQRNSELDIEARHKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCELNFDHEARLNFSEVYPATIVSQRRFAYSEVNDWLEDSDALKDESATVLESLKAGFTLSELIAEQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVAWTLTNHKVHLPYRVHPRPSKKKLQMLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVILLLRTLGKAFYIVNPLIHFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTEREQLNAELEQICETINDTEIKINGCERTANDYLTTLYLSKQVGQTFHGFISAITSFGIFMRMDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKRAILTLV" }, "paralog": false, "identity": 0.9918864097363083, "score": 965.0, "evalue": 0.0, "description": "Internal stop codon at 292857, 293874, 294123. Nonsense mutation around 292857, 293874, 294123.", "psc": { "uniref90_id": "UniRef90_P75529", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_P47350", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75529", "EC:3.1.13.1", "UniRef:UniRef50_P47350", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ] }, "pscc": { "uniref50_id": "UniRef50_P47350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47350" ], "product": "Ribonuclease R" } }, "id": "CNOCLNGIAL_553", "locus": "GCA000143945_00518" }, { "type": "cds", "sequence": "contig_1", "start": 293946, "stop": 294125, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24" ], "nt": "TTGAGCGAATTGATTGCTGAACAGCGCAAAAAGAAGGGGACTATTGACCTATCTCACAGTGAAACAGAAGTAGTCGTTGATCAAAACTACTACCCGATTGAGATCCGTTTTCTCACCCACGGCAAGGCGGAAACAATGATCGAAAACCTAATGGTGGTTGCCAATGAAGCGGTGGCTTGA", "aa": "MSELIAEQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVA", "aa_hexdigest": "077f519de39ec05950be0135225b340a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 59, "aa_cov": 0.9830508474576272, "hmm_cov": 1.0, "evalue": 2.341881445255089e-10, "score": 35.65190887451172, "start": 2, "stop": 59 } ], "seq_stats": { "molecular_weight": 6691.574899999998, "isoelectric_point": 4.951040077209471 }, "id": "CNOCLNGIAL_554", "locus": "GCA000143945_00519" }, { "type": "cds", "sequence": "contig_1", "start": 294195, "stop": 294878, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24", "PFAM:PF00575.29" ], "nt": "ATGTTGTTGGAAAACATTGTCGAGCTCAAAATTACCCAACCAAACTTTGTACTCGATACGGTCACTTCCACCCAAATTGCGGCTTGGTTAAAGGAAAATAAGGACAACCCGAGCTATGACATCTTTGTCATCTTGTTATTAAGAACGCTAGGTAAAGCCTTTTACATTGTTAATCCACTGATACACTTTAGCATTGGTTCACACCACTATACCCATTTCACTTCGCCCATCCGTCGCTATGCTGATTTAACGGTACACCGTTTGTTGTGGATGAATCTGTTTACTCCAGAGCGTTTTACTGACACTGAGCGTGAACAGCTCAACGCCGAGCTAGAACAAATTTGTGAAACCATTAACGATACTGAGATTAAGATTAATGGTTGTGAACGGACTGCCAACGATTACTTAACTACGCTGTATTTGTCTAAACAAGTGGGACAAACTTTCCACGGTTTTATTTCCGCTATTACTAGTTTTGGCATCTTTATGCGAATGGATGAAAACAACTTTGATGGACTTATTAAGATTACCTCCATTCCCGAAGACTTCTTTGTCTTTGAAAAGGATCGGATGGTGTTGCGCGGCAAACGCACCAACAAGGTTTTTAGAATTGGTGATAGGTTAACCGCGAAACTGACTGAAATTGATACAGTGCAGAAACGCGCCATTCTAACCCTGGTTTAA", "aa": "MLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVILLLRTLGKAFYIVNPLIHFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTEREQLNAELEQICETINDTEIKINGCERTANDYLTTLYLSKQVGQTFHGFISAITSFGIFMRMDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKRAILTLV", "aa_hexdigest": "bdab34d4f3f66ca21b8330091a27f971", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 75, "aa_cov": 0.32599118942731276, "hmm_cov": 0.9466666666666667, "evalue": 4.096957729516932e-21, "score": 71.00458526611328, "start": 20, "stop": 93 }, { "id": "PF00575.29", "name": "S1", "length": 82, "aa_cov": 0.36123348017621143, "hmm_cov": 0.8536585365853658, "evalue": 2.116284124149094e-11, "score": 39.560325622558594, "start": 144, "stop": 225 } ], "seq_stats": { "molecular_weight": 26401.13100000002, "isoelectric_point": 6.312959098815918 }, "id": "CNOCLNGIAL_555", "locus": "GCA000143945_00520" }, { "type": "cds", "sequence": "contig_1", "start": 294878, "stop": 295486, "strand": "+", "frame": 2, "gene": "cdaM", "product": "diadenylate cyclase CdaM", "db_xrefs": [ "COG:COG1624", "COG:T", "EC:2.7.7.85", "GO:0004016", "GO:0005524", "GO:0005886", "GO:0006171", "GO:0106408", "KEGG:K18672", "RefSeq:WP_010874601.1", "SO:0001217", "UniParc:UPI000013928C", "UniRef:UniRef100_P75528", "UniRef:UniRef50_P47351", "UniRef:UniRef90_P75528" ], "nt": "ATGATGACAGTGGAAGTCTTTTCGGTTATTATCCTTGTCCTTTTATTCTTAATCTTAGCGCTTACCTTGTTGTTTGTTTTACTCAACAAGCGTACTAGAAGCTTTGTTATTCGTACCTTTACTGGATTATTTAGATCAAAGCATACTACCAGTCAAAAGAACTTTTATGACAACTTGACCTCAACCTTACTGAGGTTATCAACTGATAAGATTGGTGCCATTATTGCCATTGAAAACCAGGACTCACTCGAGTCTTACGTCAATATTGGCTACCGCGTGACTTCGGACTTTTCCCCCGAGTTGTTAGTAACGATCTTTTACAACAAGCAGTCGCCACTTCACGATGGAGCGGTAATTGTGCGGGACTACCAGATTGTGAGTGTGTCCTCTTACTTCCCAATGACACGGCAACTAATTGATGTGTCATACGGTTCCAGACACCGCAGCGCGTTAGGTCTAACCGAAAAGTGTGATGCCATAGTCTTTATTGTTAGTGAAACAACAGGCAAAATTTCGGTGGCTGTGCGCGGCGTGATTAAAACACTGTCGAGTAACTCCGACCGTTTACAAGACCAGATTATTCACTATCTGACGGTAAAGCCGGGTTAA", "aa": "MMTVEVFSVIILVLLFLILALTLLFVLLNKRTRSFVIRTFTGLFRSKHTTSQKNFYDNLTSTLLRLSTDKIGAIIAIENQDSLESYVNIGYRVTSDFSPELLVTIFYNKQSPLHDGAVIVRDYQIVSVSSYFPMTRQLIDVSYGSRHRSALGLTEKCDAIVFIVSETTGKISVAVRGVIKTLSSNSDRLQDQIIHYLTVKPG", "aa_hexdigest": "bd9fb3567248746504c0b4dd43ffbc94", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013928C", "ncbi_nrp_id": "WP_010874601.1", "uniref100_id": "UniRef100_P75528", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013928C", "RefSeq:WP_010874601.1", "UniRef:UniRef100_P75528" ] }, "ips": { "uniref100_id": "UniRef100_P75528", "uniref90_id": "UniRef90_P75528" }, "psc": { "uniref90_id": "UniRef90_P75528", "gene": "cdaM", "product": "diadenylate cyclase CdaM", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_P47351", "cog_id": "COG1624", "cog_category": "T", "kegg_orthology_id": "K18672", "go_ids": [ "GO:0005886", "GO:0004016", "GO:0005524", "GO:0106408", "GO:0006171" ] }, "pscc": { "uniref50_id": "UniRef50_P47351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47351" ], "product": "Diadenylate cyclase" }, "genes": [ "cdaM" ], "id": "CNOCLNGIAL_556", "locus": "GCA000143945_00521" }, { "type": "cds", "sequence": "contig_1", "start": 295632, "stop": 295817, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01327.26" ], "nt": "ATGTTCTACATCCACCTAATGGATGGTGGGGTGGAACACAAGTGTCTGTTAATTAATCCCAAGATTATCAACTTAAGTGCCAACAAATCCTTTTTAAAGAGCGGTGAGGGTTGTTTGAGTGTTCCCAAAATGCACCAGGGTTATGTTATTCGCCACGAATGGATTACGATCACTGGTTTTGATTGA", "aa": "MFYIHLMDGGVEHKCLLINPKIINLSANKSFLKSGEGCLSVPKMHQGYVIRHEWITITGFD", "aa_hexdigest": "159f11430901299627617eef6d196b42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01327.26", "name": "Pep_deformylase", "length": 63, "aa_cov": 1.0, "hmm_cov": 0.9523809523809523, "evalue": 3.5240869263582827e-13, "score": 44.89892578125, "start": 1, "stop": 61 } ], "seq_stats": { "molecular_weight": 6932.0999, "isoelectric_point": 7.846785545349122 }, "id": "CNOCLNGIAL_557", "locus": "GCA000143945_00522" }, { "type": "cds", "sequence": "contig_1", "start": 295824, "stop": 296006, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTGGACGATGTTAAGGAAATTAACGAACCGACTAAACCAGTTCAGTTTCCCTTAGACCAAGCGAGTCTCGATTGCATTGCCAAAATGATGGCTTACGTTGATGCATCTTACAACGGTGATGCTGAGAAGTATGGCATTATTCCCGGCATTGGGATTGCCGCTAACCAAATTGGTTATTGA", "aa": "MLDDVKEINEPTKPVQFPLDQASLDCIAKMMAYVDASYNGDAEKYGIIPGIGIAANQIGY", "aa_hexdigest": "9066c405d33e14da462147f6c20165a3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6507.353900000002, "isoelectric_point": 4.122554969787596 }, "id": "CNOCLNGIAL_558", "locus": "GCA000143945_00523" }, { "type": "cds", "sequence": "contig_1", "start": 296077, "stop": 296298, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF00625.27" ], "nt": "ATGGTCGATACAGGGAGAATTTTTGTCATTACCGGACCGAGCGGTGTTGGCAAAAGTAGCCTTGTTAGATGTTTAATTGACCATTTCAAAGATAAACTGCGCTACAGCATTTCCGCTACCACCCGTAAAATGCGTAACAGCGAAACTGAGGGTGTGGATTACTTCTTTAAAGATAAAGCGGAGTTTGAAAAACTGATTGCTGCTGATGCCTTTGTGGAGTGA", "aa": "MVDTGRIFVITGPSGVGKSSLVRCLIDHFKDKLRYSISATTRKMRNSETEGVDYFFKDKAEFEKLIAADAFVE", "aa_hexdigest": "bb55ab2126a223b29d544b78bb1acdbb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 67, "aa_cov": 0.8767123287671232, "hmm_cov": 1.0, "evalue": 1.0434519631727448e-07, "score": 28.087451934814453, "start": 2, "stop": 65 }, { "id": "PF00625.27", "name": "Guanylate_kin", "length": 68, "aa_cov": 0.9315068493150684, "hmm_cov": 1.0, "evalue": 2.8415952308226093e-19, "score": 64.94884490966797, "start": 6, "stop": 73 } ], "seq_stats": { "molecular_weight": 8237.359199999997, "isoelectric_point": 7.869220542907715 }, "id": "CNOCLNGIAL_559", "locus": "GCA000143945_00524" }, { "type": "cds", "sequence": "contig_1", "start": 296302, "stop": 296646, "strand": "+", "frame": 1, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163", "SO:0001217", "UniRef:UniRef50_P47353", "UniRef:UniRef90_P75526" ], "nt": "ATGTATAACGATAACTACTATGGTACCCTTAAATCCCAAGCTGAACAAATTATTCACAACGGTGGCAACTTGGTGTTAGAAATTGAATATCAAGGTGCTTTACAAGTTAAGCAAAAGTATCCCAATGATGTGGTGCTAATTTTTATTAAACCACCTTCAATGGAAGAGTTGTTAGTGCGCTTAAAGAAGCGTAATGACGAGGATGCAATAACAATTCAAAACCGATTGAAACAAGCTGAGAAGGAATGTCAACAAATTGGTCACTTTAAGTATGTGGTTACCAACAACGAGTTTGACAAGACCTTAGCGGAGTTACAAGCAATTTTACTAGCTGAATTTAATTAA", "aa": "MYNDNYYGTLKSQAEQIIHNGGNLVLEIEYQGALQVKQKYPNDVVLIFIKPPSMEELLVRLKKRNDEDAITIQNRLKQAEKECQQIGHFKYVVTNNEFDKTLAELQAILLAEFN", "aa_hexdigest": "b9b1766fd16e18151b3704ab3886d132", "start_type": "ATG", "rbs_motif": null, "genes": [ "gmk" ], "truncated": "5-prime", "pseudogene": { "start": 296002, "stop": 296646, "observations": { "mutation": [ 296296 ], "stop": [ 296296 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75526", "query_cov": 1.0, "subject_cov": 0.4769874476987448, "identity": 1.0, "score": 224.0, "evalue": 1.62e-72, "gene_start": 1, "gene_end": 114, "reference_start": 126, "reference_end": 239, "reference_sequence": "MQSRLAWSKGNWTGLVGSLISLTSSSTSHALVGNNSILICGKIKIILNIEMVDTGRIFVITGPSGVGKSSLVRCLIDHFKDKLRYSISATTRKMRNSETEGVDYFFKDKAEFEKLIAADAFVEWAMYNDNYYGTLKSQAEQIIHNGGNLVLEIEYQGALQVKQKYPNDVVLIFIKPPSMEELLVRLKKRNDEDAITIQNRLKQAEKECQQIGHFKYVVTNNEFDKTLAELQAILLAEFN" }, "paralog": false, "identity": 0.9953271028037384, "score": 419.0, "evalue": 1.13e-151, "description": "Internal stop codon at 296296. Nonsense mutation around 296296.", "psc": { "uniref90_id": "UniRef90_P75526", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_P47353", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75526", "EC:2.7.4.8", "UniRef:UniRef50_P47353", "COG:COG0194", "COG:F", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_P47353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47353" ], "product": "Guanylate kinase" } }, "id": "CNOCLNGIAL_560", "locus": "GCA000143945_00525" }, { "type": "cds", "sequence": "contig_1", "start": 296646, "stop": 297020, "strand": "+", "frame": 3, "gene": "pTC1", "product": "Putative protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "GO:0017018", "GO:0033192", "GO:0140791", "GO:0180004", "GO:0180005", "GO:0180006", "GO:0180007", "GO:0180008", "GO:1990439", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_P47354", "UniRef:UniRef90_P75525" ], "nt": "ATGGACAGCACCAACCAAAACCTGTTCGCGTCATTGTCCAAAAAAGGACCCGTTCGCAAAGAAAATCAGGACTTTAGTGTTGTTACTTTCAACCGCTTTGGTCAGTTAATGAGCCTTGTTTGTGATGGTTTAGGCGGTTATAAGGGCGGTAAGATGGCTAGTGCCTTGGTGAGTGAAGTTTTTACCAAGTCCTTTACAGTCTTTGATTTTCATAGTCAAACTGAAAGGGCAGTGAAACAGTGGTTTGAAATTACTTTAATTGAAGCGCGGCGCACCTTAGAACAGTACTTTCAAACAATTAAACGTAACCAAGTCCAGTTTGCGCGAATGGCGACTACCTTAGTGTTGAGTATTATAAGTAAGCAAAACATTTGA", "aa": "MDSTNQNLFASLSKKGPVRKENQDFSVVTFNRFGQLMSLVCDGLGGYKGGKMASALVSEVFTKSFTVFDFHSQTERAVKQWFEITLIEARRTLEQYFQTIKRNQVQFARMATTLVLSIISKQNI", "aa_hexdigest": "a88e295f92a3aa202ea4fc0865483625", "start_type": "ATG", "rbs_motif": null, "genes": [ "pTC1" ], "truncated": "3-prime", "pseudogene": { "start": 296646, "stop": 297323, "observations": { "mutation": [ 297018 ], "stop": [ 297018 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75525", "query_cov": 1.0, "subject_cov": 0.47876447876447875, "identity": 1.0, "score": 242.0, "evalue": 4.74e-79, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MDSTNQNLFASLSKKGPVRKENQDFSVVTFNRFGQLMSLVCDGLGGYKGGKMASALVSEVFTKSFTVFDFHSQTERAVKQWFEITLIEARRTLEQYFQTIKRNQVQFARMATTLVLSIISKQNIWTFWVGDSRAYLINSYQSLQITEDHNLYNQLLQMHATPDVIASYKDKLLALTATVSKDQERQLKYSFRCDVVNAWDFLLLCSDGLYNFLDPNCFYEVITSAPNLKKAVTQLAKLSLDNASNDNITLNLINLKQWH" }, "paralog": false, "identity": 0.9955555555555555, "score": 447.0, "evalue": 2.64e-162, "description": "Internal stop codon at 297018. Nonsense mutation around 297018.", "psc": { "uniref90_id": "UniRef90_P75525", "gene": "PTC1", "product": "Putative protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_P47354", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "go_ids": [ "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75525", "EC:3.1.3.16", "UniRef:UniRef50_P47354", "COG:COG0631", "COG:T", "KEGG:K20074", "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ] }, "pscc": { "uniref50_id": "UniRef50_P47354", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47354" ], "product": "Putative protein phosphatase" } }, "id": "CNOCLNGIAL_561", "locus": "GCA000143945_00526" }, { "type": "cds", "sequence": "contig_1", "start": 297030, "stop": 297425, "strand": "+", "frame": 3, "gene": "pTC1", "product": "Putative protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "GO:0017018", "GO:0033192", "GO:0140791", "GO:0180004", "GO:0180005", "GO:0180006", "GO:0180007", "GO:0180008", "GO:1990439", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_P47354", "UniRef:UniRef90_P75525" ], "nt": "GTGGGTGATTCACGTGCTTATTTAATTAACAGTTACCAAAGCTTGCAAATTACCGAAGACCATAACCTGTACAACCAACTGTTACAGATGCACGCTACCCCTGATGTGATTGCCTCTTATAAAGACAAGCTGTTAGCCCTCACTGCTACCGTTTCCAAAGATCAGGAACGCCAGTTGAAATACAGCTTTAGGTGTGATGTAGTAAACGCTTGGGACTTTTTACTGTTGTGCTCTGATGGTCTGTACAACTTCTTGGATCCCAACTGCTTTTATGAGGTCATTACTAGTGCACCTAACCTCAAAAAGGCCGTTACACAACTAGCAAAGTTGAGCTTGGATAACGCTAGTAATGACAACATTACGCTCAACTTGATTAATTTAAAACAATGGCACTAA", "aa": "MGDSRAYLINSYQSLQITEDHNLYNQLLQMHATPDVIASYKDKLLALTATVSKDQERQLKYSFRCDVVNAWDFLLLCSDGLYNFLDPNCFYEVITSAPNLKKAVTQLAKLSLDNASNDNITLNLINLKQWH", "aa_hexdigest": "cdfd83a2d804c7344bee24f8c1941817", "start_type": "GTG", "rbs_motif": null, "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 296730, "stop": 297425, "observations": { "mutation": [ 297018 ], "stop": [ 297018 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75525", "query_cov": 1.0, "subject_cov": 0.5057915057915058, "identity": 0.992, "score": 265.0, "evalue": 4.78e-88, "gene_start": 1, "gene_end": 131, "reference_start": 129, "reference_end": 259, "reference_sequence": "MDSTNQNLFASLSKKGPVRKENQDFSVVTFNRFGQLMSLVCDGLGGYKGGKMASALVSEVFTKSFTVFDFHSQTERAVKQWFEITLIEARRTLEQYFQTIKRNQVQFARMATTLVLSIISKQNIWTFWVGDSRAYLINSYQSLQITEDHNLYNQLLQMHATPDVIASYKDKLLALTATVSKDQERQLKYSFRCDVVNAWDFLLLCSDGLYNFLDPNCFYEVITSAPNLKKAVTQLAKLSLDNASNDNITLNLINLKQWH" }, "paralog": false, "identity": 0.9956709956709957, "score": 460.0, "evalue": 2.3e-167, "description": "Internal stop codon at 297018. Nonsense mutation around 297018.", "psc": { "uniref90_id": "UniRef90_P75525", "gene": "PTC1", "product": "Putative protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_P47354", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "go_ids": [ "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75525", "EC:3.1.3.16", "UniRef:UniRef50_P47354", "COG:COG0631", "COG:T", "KEGG:K20074", "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ] }, "pscc": { "uniref50_id": "UniRef50_P47354", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47354" ], "product": "Putative protein phosphatase" } }, "id": "CNOCLNGIAL_562", "locus": "GCA000143945_00527" }, { "type": "cds", "sequence": "contig_1", "start": 297416, "stop": 298384, "strand": "+", "frame": 2, "gene": "sPS1", "product": "Putative serine/threonine-protein kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "EC:2.7.11.1", "GO:0004676", "GO:0004677", "GO:0004679", "GO:0004711", "GO:0005524", "GO:0035175", "GO:0035402", "GO:0035403", "GO:0035979", "GO:0044022", "GO:0044023", "GO:0044024", "GO:0044025", "GO:0072354", "GO:0072371", "GO:0072518", "GO:0106310", "GO:0140823", "GO:0140855", "GO:0140857", "GO:1990244", "SO:0001217", "UniRef:UniRef50_P47355", "UniRef:UniRef90_P75524" ], "nt": "ATGGCACTAAATTTAAAGATTGGTGACATTGTCCAAAACAAGTACCGGATTGAAAAGCTAATCAACCGTGGCGGGATGAACTCTTACCTGTTTTTAGCGAGCAACCTCCACGTCCAGGAATTTGGTCCACTGCAAAAGCGCCAGTTTACCCGGTTGGTGTTAAAGGTAGTACAACGCACCGACAAGATTAACGACAACAACTGGAAGAAGTTTTTGGATGAAACCATTACCACGACACGGGTGTCACACAAAAATCTGGTGCAAACCTTTGATGTAGTTAGCCCCCGTTTAAGCGTTTTGAGTGAAAACCAGGTGATCGTTTTAGAGGACACGGTGATGATTGTAATGGAGTATGTTGATGGTCCTTCTTTGCGTGAAATGCTCAACCAGAAGGGTTACTTTAGTGTGCAGGAGGTGGTGTACTACTTTACAAAGCTAGTCAAAGTAATTAACTACCTCCACAGTTTTGAACACCAAATTATTCACCGTGATCTAAAGCCAGAAAACATCCTGTTTACGAGTAACTTGACTGACATTAAACTGCTTGACTTTGGCATTGCTTCCGCTGTTATTCGTAACGCTGAGAAAACTGAAGTGTTAACGGACGAAAACTCGTTGTTTGGTACAGTTAGTTATATGACACCCGAAGTGTTGGAAAGTACTGTCAACAAAGAAGGCAAACGGATTCGTAAACCCCCTACAGTTCAGTATGACATCTATTCATTAGGAGTTATTTTGTTTGAAATGTTAGTCGGACGCGTACCGTTCAATAAATCGATTGATCCTAAAAAAGAACGGGAAACCATCCAAAAGGCGCGTAACTTTGATGTACCTTTAATGGGAAACTTGCGCAGTGATGTTCCGGTTAGTTTGGAAAACATTGTCTTTAAATGTACGGCTGTTAAACGTGAAAACAGTAAGTGGATGTATAGTGACACGAAGCAACTCTTGGCTGATTTGGCGCAATGA", "aa": "MALNLKIGDIVQNKYRIEKLINRGGMNSYLFLASNLHVQEFGPLQKRQFTRLVLKVVQRTDKINDNNWKKFLDETITTTRVSHKNLVQTFDVVSPRLSVLSENQVIVLEDTVMIVMEYVDGPSLREMLNQKGYFSVQEVVYYFTKLVKVINYLHSFEHQIIHRDLKPENILFTSNLTDIKLLDFGIASAVIRNAEKTEVLTDENSLFGTVSYMTPEVLESTVNKEGKRIRKPPTVQYDIYSLGVILFEMLVGRVPFNKSIDPKKERETIQKARNFDVPLMGNLRSDVPVSLENIVFKCTAVKRENSKWMYSDTKQLLADLAQ", "aa_hexdigest": "f8adeb2f37efb586f36c8ba3d21b2912", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75524", "query_cov": 1.0, "subject_cov": 0.8277634961439588, "identity": 0.997, "score": 625.0, "evalue": 4.83e-225, "valid": true, "gene": "SPS1", "product": "Putative serine/threonine-protein kinase", "ec_ids": [ "2.7.11.1" ], "uniref50_id": "UniRef50_P47355", "cog_id": "COG0515", "cog_category": "T", "go_ids": [ "GO:0004676", "GO:0004679", "GO:0005524", "GO:0004677", "GO:0044024", "GO:0072371", "GO:1990244", "GO:0035979", "GO:0044025", "GO:0140823", "GO:0035175", "GO:0044022", "GO:0140855", "GO:0035402", "GO:0072354", "GO:0140857", "GO:0035403", "GO:0044023", "GO:0106310", "GO:0072518", "GO:0004711" ] }, "pscc": { "uniref50_id": "UniRef50_P47355", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47355" ], "product": "Putative serine/threonine-protein kinase" }, "genes": [ "sPS1" ], "id": "CNOCLNGIAL_563", "locus": "GCA000143945_00528" }, { "type": "cds", "sequence": "contig_1", "start": 298412, "stop": 298585, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCACGAACGTATTCTAGAAGGACAAAACGAAATGCGTGAACTAATGGTAAGTAATTACCTACCGTGGTATTTAAGGAAGGGTGTTTTGATCTTTTTTTCTGTTGTTTTATTAGCACTCTTAATTGCTGTAGTTAGCTTTTTTATTATTACTGGGGTAGTTGTCCATTCGTAA", "aa": "MHERILEGQNEMRELMVSNYLPWYLRKGVLIFFSVVLLALLIAVVSFFIITGVVVHS", "aa_hexdigest": "1b2f91b1dccc1b0c699623e34bd32845", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6592.876599999998, "isoelectric_point": 6.70656909942627 }, "id": "CNOCLNGIAL_564", "locus": "GCA000143945_00529" }, { "type": "cds", "sequence": "contig_1", "start": 298845, "stop": 299375, "strand": "+", "frame": 3, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872", "KEGG:K06949", "SO:0001217", "UniRef:UniRef50_P75523", "UniRef:UniRef90_P75523" ], "nt": "TTGTTTAAATTGTTAATCCATTTTCACGCTCAAAACGTGGCACGGTTAGTGTTAGTCATTACTAAAAACGACCTTGAGTTTGAACCTGCTGAAAAAGCCCGTTTAAGTGAACTAACCAGCTTTGGTTACCAACTCTTTTTTACAGCCCCTAATGCTGATTTACCTCTCACTTTATTTACAGAATTGCAAAACCGTTTTAGTGTATTCATGGGACAATCAGGGGTCGGTAAGTCTAGCCTTATTAACCGTTTGGACAGTCAAATTCACCAAGCGATCCAAGCGCTTTCAGCACACCAGTTCGGTAAAAACACCACCACTTCCACGGTGATGTTTCCCTTCCAAAACGGCTTTATTTGTGACACCCCTGGCTTTAATGTAATTGACTTTCCCAACCTCAAACAATTAGCAGCCCAACACTTTGTCGGTTTTGCTAGCTTAATTGGTCAATGCCATTTTTCCAACTGCACCCATCAAAGCGAAAAAGGTTGTTTTATTGTAAGTGCTGTAACCCAAAAATCGTATTCCTTGTGA", "aa": "MFKLLIHFHAQNVARLVLVITKNDLEFEPAEKARLSELTSFGYQLFFTAPNADLPLTLFTELQNRFSVFMGQSGVGKSSLINRLDSQIHQAIQALSAHQFGKNTTTSTVMFPFQNGFICDTPGFNVIDFPNLKQLAAQHFVGFASLIGQCHFSNCTHQSEKGCFIVSAVTQKSYSL", "aa_hexdigest": "8fb81df9a2babc17ea5d87eaf5e50f5f", "start_type": "TTG", "rbs_motif": null, "genes": [ "rsgA" ], "truncated": "both", "pseudogene": { "start": 298572, "stop": 299408, "observations": { "mutation": [ 298677, 298836, 299373 ], "stop": [ 298677, 298836, 299373 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75523", "query_cov": 1.0, "subject_cov": 0.6330935251798561, "identity": 0.9940000000000001, "score": 352.0, "evalue": 2.33e-121, "gene_start": 1, "gene_end": 176, "reference_start": 92, "reference_end": 267, "reference_sequence": "MSIRKTGIVFQRFGKQFKVFVENQTLVAFAQKKLQWKRDFKLLVGDKVTLENGVIVAVEERKNTLVRPKVVNVDQVVIVQSLIEPKINWQQLFKLLIHFHAQNVARLVLVITKNDLEFEPAEKARLSELTSFGYQLFFTAPNADLPLTLFTELQNRFSVFMGQSGVGKSSLINRLDSQIHQAIQALSAHQFGKNTTTSTVMFPFQNGFICDTPGFNVIDFPNLKQLAAQHFVGFASLIGQCHFSNCTHQSEKGCFIVSAVTQKSYSLWLYESYLKLIN" }, "paralog": false, "identity": 0.9856115107913669, "score": 535.0, "evalue": 7.6e-196, "description": "Internal stop codon at 298677, 298836, 299373. Nonsense mutation around 298677, 298836, 299373.", "psc": { "uniref90_id": "UniRef90_P75523", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.6.1.-" ], "uniref50_id": "UniRef50_P75523", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75523", "EC:3.6.1.-", "UniRef:UniRef50_P75523", "COG:COG1162", "COG:J", "KEGG:K06949", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75523" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" } }, "id": "CNOCLNGIAL_565", "locus": "GCA000143945_00530" }, { "type": "cds", "sequence": "contig_1", "start": 299430, "stop": 299822, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00342.24" ], "nt": "ATGGAAAGTAAATGGTTAACAGTTGACACTAAGCACCTCTATGGTTTTGATGAAGCGATTTTTATTCAGAAATACCAAAAAAAAGTTAACCAAATTCACCAACAGTTTTTAAATCAACAACTACCCGATGGTCACATGAATGGCTGGTATAGTCAACCCGATCAAGACCACAAGGGTTTGCTAAAACAAATTAACACAATTGCCAAACAGTTTAATGCTCTAAAAGTAACTGACATAGTGTATTTAGGTATTGGTGGTTCTTATACTGGCATTCGTGCCATCTTAGACTTTTTAAAACCAGAACAGAAGGCGAATATCAAAGTGCACTTTGTTCCTGACATATCGGCCTTTAACATTGCTGCAGTTGCTAAAGCAATTAAGGGTAAATCATGA", "aa": "MESKWLTVDTKHLYGFDEAIFIQKYQKKVNQIHQQFLNQQLPDGHMNGWYSQPDQDHKGLLKQINTIAKQFNALKVTDIVYLGIGGSYTGIRAILDFLKPEQKANIKVHFVPDISAFNIAAVAKAIKGKS", "aa_hexdigest": "093b57f5ee34d0a00a2c8b155210631a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00342.24", "name": "PGI", "length": 55, "aa_cov": 0.4230769230769231, "hmm_cov": 0.9818181818181818, "evalue": 4.93753622103886e-07, "score": 24.126863479614258, "start": 73, "stop": 127 } ], "seq_stats": { "molecular_weight": 14757.835499999981, "isoelectric_point": 9.398410606384278 }, "id": "CNOCLNGIAL_566", "locus": "GCA000143945_00531" }, { "type": "cds", "sequence": "contig_1", "start": 299826, "stop": 300185, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGTAACTTCTAAATCTGGTCGCACACTCGAGCCGGCTGTTACCTTTCGGTACTTCCGTAATTTATTGCACAAACAATACAAGCAAAAACATGCTTTACGCACAGTTGTGATTACCGATGCCGTTAAGGGTTTACTAGTTGGCATGAGTAACCAGTACGGTTATGCACACTTAACTATTCCAAGCAATATTGGTGGGCGTTTTTCAACTCTTTCACCCGCCGGTTTACTGTTAGCTAAACTTTGTGGTCATGATCCGAAACAGCTCTTATTGGGTACTTTGACAGCCAAACAAGAGTTAGCAAACAGTGATTTAAACACTAATTCGGCTTATTATTATGCTGCTTTGCGTCACTGA", "aa": "MVVTSKSGRTLEPAVTFRYFRNLLHKQYKQKHALRTVVITDAVKGLLVGMSNQYGYAHLTIPSNIGGRFSTLSPAGLLLAKLCGHDPKQLLLGTLTAKQELANSDLNTNSAYYYAALRH", "aa_hexdigest": "41cde99de6a42c799c7e91b033a01f86", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13119.045899999983, "isoelectric_point": 9.947874641418458 }, "id": "CNOCLNGIAL_567", "locus": "GCA000143945_00532" }, { "type": "cds", "sequence": "contig_1", "start": 300216, "stop": 300722, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACGGTAGCATACCACAGTGCTTATGAGTACCTCTTGTTGCAACACCGCCAACTGTTCGGTGAATCAGAGGGTAAAGGTGGTAAGTCCCTGTTCCCTACCTTTTCGCTATTTACCACTGACTTACACTCAATGGGGCAGTTGTACCAAGAAGGGGAAAAGAACTTCTTTGAAACAGTGATACAAGTGCAAACACAATTTCACGACTTGGAACTACCACCATCAGACTTTAACAATGATGATCAGCTAGATTACTTATTGGCTAAAAGTATGAATGAAATTTCAAACACTGCCCTAGAAGCAGTTGTGGAAGCTCATTTTCAATCAAACGTTAACATCATTAAGTTAACGCTCAAAGAAAGAACAACCTTTATGTTTGGTTACTTTTACTTCTGGTTATCAATGGCCACAATGATGAGTGGTTCACTGCTGGGACACAACGTCTTTGATCAACCAGGGGTGGAAGTGTACAAGCAGTTAATGTTTGCTAAACTTGGTCGTGAATAG", "aa": "MTVAYHSAYEYLLLQHRQLFGESEGKGGKSLFPTFSLFTTDLHSMGQLYQEGEKNFFETVIQVQTQFHDLELPPSDFNNDDQLDYLLAKSMNEISNTALEAVVEAHFQSNVNIIKLTLKERTTFMFGYFYFWLSMATMMSGSLLGHNVFDQPGVEVYKQLMFAKLGRE", "aa_hexdigest": "966487e1881d2146614e255c06480633", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19352.730699999975, "isoelectric_point": 5.042266654968262 }, "id": "CNOCLNGIAL_568", "locus": "GCA000143945_00533" }, { "type": "cds", "sequence": "contig_1", "start": 300715, "stop": 301347, "strand": "+", "frame": 1, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "EC:5.1.3.1", "GO:0004750", "GO:0006098", "GO:0019323", "GO:0046872", "SO:0001217", "UniParc:UPI0001DD64FF", "UniRef:UniRef100_A0AB38W7C5", "UniRef:UniRef50_P47358", "UniRef:UniRef90_P75522" ], "nt": "GTGAATAGGGAAATTGCCTTTTCGCTTTTACCACTGTTACACCAGTTTGACCGCAAGTTGTTAGAACAATTCTTTGCGGACGGCTTGCGCTTAATCCACTATGACGTGATGGACCATTTTGTGGACAACACTGTCTTTCAAGGGGAACATTTAGATGAATTACAACAAATTGGTTTTCAGGTCAATGTACATTTGATGGTACAAGCATTAGAACAAATCTTGCCAGTATACCTCCACCATCAAGCCGTTAAGCGTATTTCCTTTCACGTTGAACCGTTTGATATCCCTACCATCAAACACTTTATTGCGCAAATTAAACAAGCGGGAAAACAAGTCGGTTTAGCGTTTAAATTCACCACTCCGCTTGTAAATTACGAGCGCTTGGTGCAACAACTGGACTTTGTTACCTTAATGAGTGTCCCCCCAGGAAAGGGCGGGCAAGCCTTTAACAGTGCTGTTTTTAATAACTTAAAACAAGCCCACAAATACCACTGCTCAATTGAAATCGATGGTGGGATCAAATTAGATAACATTCACCAAATCCAGGATGATGTCAATTTTATTGTCATGGGAAGTGGCTTTATAAAATTAGAACGATGGCAGCGCCAACAGCTGCTCAAAACCAATCAATAA", "aa": "MNREIAFSLLPLLHQFDRKLLEQFFADGLRLIHYDVMDHFVDNTVFQGEHLDELQQIGFQVNVHLMVQALEQILPVYLHHQAVKRISFHVEPFDIPTIKHFIAQIKQAGKQVGLAFKFTTPLVNYERLVQQLDFVTLMSVPPGKGGQAFNSAVFNNLKQAHKYHCSIEIDGGIKLDNIHQIQDDVNFIVMGSGFIKLERWQRQQLLKTNQ", "aa_hexdigest": "179887d09df0e062c70b45ea8ae49ba4", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64FF", "uniref100_id": "UniRef100_A0AB38W7C5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64FF", "UniRef:UniRef100_A0AB38W7C5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7C5", "uniref90_id": "UniRef90_P75522" }, "psc": { "uniref90_id": "UniRef90_P75522", "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "ec_ids": [ "5.1.3.1" ], "uniref50_id": "UniRef50_P47358", "cog_id": "COG0036", "cog_category": "G", "go_ids": [ "GO:0004750", "GO:0046872", "GO:0019323", "GO:0006098" ] }, "pscc": { "uniref50_id": "UniRef50_P47358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47358" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [ "rpe" ], "id": "CNOCLNGIAL_569", "locus": "GCA000143945_00534" }, { "type": "cds", "sequence": "contig_1", "start": 301432, "stop": 302721, "strand": "+", "frame": 1, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_P47359", "UniRef:UniRef90_P75521" ], "nt": "TTGAAAAACAAACGTACTAGCGCCAACATTATCTTTTTGGAAGTTAATGATGGATCAACGCTGTTAAACCTACAAGCGGTGGTTAAACAAGACCAACCGGAGTTGTTTGCATTAGCTGAATCAATTAGTTTAGCGAGTGCTGTAAGTGTTTCTGGTACTGTAGCGTTAACACCCAAATCCAAACAACCCTTGGAATTAGTAGTCAAACAAATTAATGTATTGAGTACTGCGCGAGCAGACTATCCACTCCAAAAAAAGGAACACAGCTTGGAGTTTTTCCGCAACAACGCCTATTTGCGCGTACGAGCCCGTACCTACTTTGCCATCATGAAAGTGCGCAGTCTGTTGTCACAAGCCATTTTTGACTACTTCTTTAAGAATGACTTTGTTTTAGTGCACAGTCCAATTCTTACTAGCAACGACTGTGAGGGGGCAGGTGAGACCTTTGAATTAAAGCAAGGTAAGGAATTCTTTAATAAGACTACTTACCTTACTGTCAGTGGTCAGTTTGGTGCGGAGTGTTACGCCCAAGCCTTTAAAAAAGTGTTTACCTTCGGGCCGACTTTTCGCGCTGAAAAATCACACACTTCACGGCACCTTAGTGAGTTTTGGATGATTGAACCAGAAGTTGCTTTTGCCAACCTCAAGGACTTAATTAAGCTAATTGAAAGCACTGTCAAAACCGTGATTAAACAGGTTATGCAAAAAGCCAAGCAGGAACTCGACTTTTTAGAAAAGCAGTTTGATGTCAAATTAATGGAACGCTTAAAGCAAATTACAAGTACCAAAAACTTCCATGTTTTGGAATACATCAAGGCCTTAGAGATTTTAAAAACAGCCCAAGCTAGTGGTCAAGCTAACTTTGAGGTACAAGACTTTAACTTCGGTTTAGATCTCAAAACGGAACACGAACGCTTTTTGTGTGAACAGCATTTTCACAACCAACCGGTGTTTGTAATTAACTACCCCAAGGACCTTAAGGCCTTTTACATGAAGCAAAATGCTGATGGTAGAACTGTTGGTGCGGTTGACCTTTTATTTCCCCAAATTGGTGAGATCTGTGGTGGGAGTGAACGGGAAGGTAATCTAGAAAAACTAGTGGAACGTTGCCAAGCAATGCAAATTGATACCCAAACACTTAACTGGTATTTAGATATGCGTAAATGGGGTTACTTTGCCTCAGCTGGCTTTGGTTTAGGGTTTGATCGTTTACTAGCTTACATCTGTGGATTGGAAAACATCCGTGATGCGATCCCCTTTCCCCGTGCCCATGGTTCCATTAACTATTAA", "aa": "MKNKRTSANIIFLEVNDGSTLLNLQAVVKQDQPELFALAESISLASAVSVSGTVALTPKSKQPLELVVKQINVLSTARADYPLQKKEHSLEFFRNNAYLRVRARTYFAIMKVRSLLSQAIFDYFFKNDFVLVHSPILTSNDCEGAGETFELKQGKEFFNKTTYLTVSGQFGAECYAQAFKKVFTFGPTFRAEKSHTSRHLSEFWMIEPEVAFANLKDLIKLIESTVKTVIKQVMQKAKQELDFLEKQFDVKLMERLKQITSTKNFHVLEYIKALEILKTAQASGQANFEVQDFNFGLDLKTEHERFLCEQHFHNQPVFVINYPKDLKAFYMKQNADGRTVGAVDLLFPQIGEICGGSEREGNLEKLVERCQAMQIDTQTLNWYLDMRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRAHGSINY", "aa_hexdigest": "c5a00f7bd214d3f146213dfe2a3f1015", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75521", "query_cov": 1.0, "subject_cov": 0.9428571428571428, "identity": 0.993, "score": 842.0, "evalue": 9.91e-308, "valid": true, "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_P47359", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_P47359", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47359" ], "product": "Asparagine--tRNA ligase" }, "genes": [ "asnS" ], "id": "CNOCLNGIAL_570", "locus": "GCA000143945_00535" }, { "type": "cds", "sequence": "contig_1", "start": 302996, "stop": 303154, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATTAACGGTGTCTTAATTGTCCTAGTAGCATATGGCTACTTTCACTTTAGTTTTTTAATTGTGATTGTGCTGCGTGATTTAGTGCTAGACGGATTGCGCTTTTACGCTCAGGAAAAACAGCTAATTATTCCCGCCAACCAATGGGGTAAGTGA", "aa": "MIINGVLIVLVAYGYFHFSFLIVIVLRDLVLDGLRFYAQEKQLIIPANQWGK", "aa_hexdigest": "54e7496a4971a6b611a7e82135b38c35", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6009.154299999999, "isoelectric_point": 8.16035976409912 }, "id": "CNOCLNGIAL_571", "locus": "GCA000143945_00536" }, { "type": "cds", "sequence": "contig_1", "start": 303260, "stop": 303361, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTTGCCCTATTATTTAGCTACAGCCTTCTCCTTGGTCTCGTTTGGTATTTACGCCCAACAAATATATAAAACCATTAAGGTTAAAGTAAAGTTATAA", "aa": "MHLPYYLATAFSLVSFGIYAQQIYKTIKVKVKL", "aa_hexdigest": "b18b99d24366c3ffd08f935b0d49e79d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3835.5974000000006, "isoelectric_point": 9.778580665588379 }, "id": "CNOCLNGIAL_572", "locus": "GCA000143945_00537" }, { "type": "cds", "sequence": "contig_1", "start": 303383, "stop": 303856, "strand": "+", "frame": 2, "gene": "pncC", "product": "Protein MG115-like protein", "db_xrefs": [ "COG:COG1546", "COG:H", "EC:3.5.1.42", "KEGG:K03743", "RefSeq:WP_014325421.1", "SO:0001217", "UniParc:UPI0001DD6502", "UniRef:UniRef100_A0A0H3DKL5", "UniRef:UniRef50_P47361", "UniRef:UniRef90_Q9EXC9" ], "nt": "GTGTACGCGCGCTTAATTGCTGAAAAACTGCTAAATCATAAGCTCACCATTGCCACTGCAGAGTCAGTTACTGGGGGCCTTTTGAGTAGTAGCTTAACCGACATCGCTGGTGCTTCTCGTTTCTTTAAGGGTGCCATTGTGGCATACAGCAATGAATTGAAAAAATCACTTTTAAATGTCAAACAATCAACCTTAATTAACCACGGTGCTGTATCGCGTTATTGTGTTCGTGAAATGGCGTTAGGTTTAATGCAAAAATTCAATGTTGATATTGCCGTGGCCTGCAGTGGGTTAGCGGGTCCTGATGCATTGGAAAACCAAGCAGTTGGTAGCTTGTTTTTCTGTGTGATTGTTGCCAACAAGGCTTATGACTTTGAAACAAAACTACCAGCTGGCTCTAGAAACGAATTGCGCCAGCTTTTTGTGCAAAAAATCTTGCAAACAGTTGAACACATTTTAAGTGAAATTTCTTAG", "aa": "MYARLIAEKLLNHKLTIATAESVTGGLLSSSLTDIAGASRFFKGAIVAYSNELKKSLLNVKQSTLINHGAVSRYCVREMALGLMQKFNVDIAVACSGLAGPDALENQAVGSLFFCVIVANKAYDFETKLPAGSRNELRQLFVQKILQTVEHILSEIS", "aa_hexdigest": "ff2963340c8573ce9368a56cf29ef3ea", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6502", "ncbi_nrp_id": "WP_014325421.1", "uniref100_id": "UniRef100_A0A0H3DKL5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6502", "RefSeq:WP_014325421.1", "UniRef:UniRef100_A0A0H3DKL5" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DKL5", "uniref90_id": "UniRef90_Q9EXC9" }, "psc": { "uniref90_id": "UniRef90_Q9EXC9", "gene": "PncC", "product": "Protein MG115 homolog", "ec_ids": [ "3.5.1.42" ], "uniref50_id": "UniRef50_P47361", "cog_id": "COG1546", "cog_category": "H", "kegg_orthology_id": "K03743" }, "pscc": { "uniref50_id": "UniRef50_P47361", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47361" ], "product": "Protein MG115" }, "genes": [ "pncC" ], "id": "CNOCLNGIAL_573", "locus": "GCA000143945_00538" }, { "type": "cds", "sequence": "contig_1", "start": 303882, "stop": 304637, "strand": "+", "frame": 3, "gene": "ispD", "product": "putative MG116-like protein", "db_xrefs": [ "COG:COG1211", "COG:I", "RefSeq:WP_010874612.1", "SO:0001217", "UniParc:UPI00001392BE", "UniRef:UniRef100_P75519", "UniRef:UniRef50_P75519", "UniRef:UniRef90_P75519" ], "nt": "ATGCAGCGTACTTTAAATGTTGGGGTGATCCTGTGTGAAAGCTTTATTTCCAACGCCCAAAACCCAGTTAACTCCTACATTAAGATTTACGAAAACGTGAGGATGTTTGAGTTTGCGATTAAGCTACTTCAAGAATCGCGAATTAACTTCCAAAAGATCCTTTTATACGTTTTGGAAGAGCAAATTCCACTTGTGGAAAAAGTAGTTGCCAAGTACGAAAACTGCTGGGTCTTTCGTTCCAAGCACAACGAGGTTGAGGACATTTATGAAGCCAAGGGCTTTATTGAAGACAAGTACAAAATTGGTTTAAAAAGTTCTAAAAACCAAGCCTCTTCGTATTATGACAACTGTTGTTTTGTTGTCTTGGAAGCATGTCGACCTTTAACTTCCAAGAAGGTTGTTAAAAACGTGTATGAAAAGGCAATGATTGACGGTGCCGCTGTAGCTGTACTACCGTTTGAAAGGCAGCTTGTTTGTGGTGATAATACTAAGGCGGTGCGCCAGTTAAAGGACAAGGGCAACATGAACTATTGGCGTCAAAGTAGTACTTGGGAGTTGCAATTTCCCCAGGCTTATACGCTCAATAAGTTAAACCAGTATCATAAAAACCTCTTTATGAAGGCGCGGAACATGCTTGATTTAATGAACATTTCCGCTAAGAATCCCCTAAGCATTGTCGATGGTAGTGCTTACAGCTTTCGTGTAGTTACTAGCCTGGACTTTGAAATTTTGTTAGGTATTTTGCGCAATGGATAG", "aa": "MQRTLNVGVILCESFISNAQNPVNSYIKIYENVRMFEFAIKLLQESRINFQKILLYVLEEQIPLVEKVVAKYENCWVFRSKHNEVEDIYEAKGFIEDKYKIGLKSSKNQASSYYDNCCFVVLEACRPLTSKKVVKNVYEKAMIDGAAVAVLPFERQLVCGDNTKAVRQLKDKGNMNYWRQSSTWELQFPQAYTLNKLNQYHKNLFMKARNMLDLMNISAKNPLSIVDGSAYSFRVVTSLDFEILLGILRNG", "aa_hexdigest": "ffac60a049bc33a21b2f7b4654c78aad", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001392BE", "ncbi_nrp_id": "WP_010874612.1", "uniref100_id": "UniRef100_P75519", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001392BE", "RefSeq:WP_010874612.1", "UniRef:UniRef100_P75519" ] }, "ips": { "uniref100_id": "UniRef100_P75519", "uniref90_id": "UniRef90_P75519" }, "psc": { "uniref90_id": "UniRef90_P75519", "gene": "IspD", "product": "Uncharacterized protein MG116 homolog", "uniref50_id": "UniRef50_P75519", "cog_id": "COG1211", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_P75519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75519" ], "product": "Uncharacterized protein MG116 homolog" }, "genes": [ "ispD" ], "id": "CNOCLNGIAL_574", "locus": "GCA000143945_00539" }, { "type": "cds", "sequence": "contig_1", "start": 304630, "stop": 304854, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGAAAAATAGTTCAACTAATTCATAACTTTAGCGGTACAGAAAAACTAAAAGCAGCTGTTTTTTCACACGACGAACAGTGTTTCTTTGACTTTGTTAGCTTCGATGAATTAAACAGTAAGCTAACTGGGTTTCTGTTATTTGACAGCTTGGAGAAGCTGTTCAAACTAGTAGAAACAATTCAGCAAAAGCGCAGTTGGCTTTATGTTGATGAATTGTGA", "aa": "MDRKIVQLIHNFSGTEKLKAAVFSHDEQCFFDFVSFDELNSKLTGFLLFDSLEKLFKLVETIQQKRSWLYVDEL", "aa_hexdigest": "86d92816ff1c1965dd3457d22073473c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8777.983299999996, "isoelectric_point": 5.2073270797729485 }, "id": "CNOCLNGIAL_575", "locus": "GCA000143945_00540" }, { "type": "cds", "sequence": "contig_1", "start": 304909, "stop": 305289, "strand": "+", "frame": 1, "gene": null, "product": "MPN256 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75518", "UniRef:UniRef90_P75518" ], "nt": "GTGAAAAAGGAGCTTGCGCAAGTCGGTGTTTTAACCCAATTGGATACTAGTTTAGTCAAGCTTTTAATGGCTTCCAAAAATACTGACAGTGCCCTTTACAACACTTACATTAAACCAGTGGAGTTGCAACAGTTTACCCAAACACCAGCTCCTGATAACGTTAATGCAGAGCAATCGCATTTAACCTTGGAGTCTACCACCGACTTGAACAACAGTCAACTAGCCAATACTCCAGCGTTGTGGGAAGTGGAACAAACGACCCAGGAATTGTTACCAACCATGGACTTTTCTAAGTTTATTGATGAATTAGATCAAATTACCAAAAACTTTTCTGATTTAGAGTTAGAACCACTTAGTTTTAACGAAGGTTTTGATGAGTGA", "aa": "MKKELAQVGVLTQLDTSLVKLLMASKNTDSALYNTYIKPVELQQFTQTPAPDNVNAEQSHLTLESTTDLNNSQLANTPALWEVEQTTQELLPTMDFSKFIDELDQITKNFSDLELEPLSFNEGFDE", "aa_hexdigest": "901fcacd9c68733468e150ab4407c3d8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 304630, "stop": 305301, "observations": { "mutation": [ 304852, 305287 ], "stop": [ 304852, 305287 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75518", "query_cov": 0.9920634920634921, "subject_cov": 0.5605381165919282, "identity": 1.0, "score": 236.0, "evalue": 2.15e-77, "gene_start": 2, "gene_end": 126, "reference_start": 95, "reference_end": 219, "reference_sequence": "MDRKIVQLIHNFSGTEKLKAAVFSHDEQCFFDFVSFDELNSKLTGFLLFDSLEKLFKLVETIQQKRSWLYVDELWLINTATENQQLNEVSVWLVKKELAQVGVLTQLDTSLVKLLMASKNTDSALYNTYIKPVELQQFTQTPAPDNVNAEQSHLTLESTTDLNNSQLANTPALWEVEQTTQELLPTMDFSKFIDELDQITKNFSDLELEPLSFNEGFDEWNQE" }, "paralog": false, "identity": 0.9865470852017937, "score": 432.0, "evalue": 4.73e-157, "description": "Internal stop codon at 304852, 305287. Nonsense mutation around 304852, 305287.", "psc": { "uniref90_id": "UniRef90_P75518", "product": "MPN256 family protein", "uniref50_id": "UniRef50_P75518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75518", "UniRef:UniRef50_P75518" ] }, "pscc": { "uniref50_id": "UniRef50_P75518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75518" ], "product": "Uncharacterized protein MG117 homolog" } }, "id": "CNOCLNGIAL_576", "locus": "GCA000143945_00541" }, { "type": "cds", "sequence": "contig_1", "start": 305282, "stop": 306019, "strand": "+", "frame": 2, "gene": null, "product": "NAD-dependent epimerase/dehydratase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D" ], "nt": "ATGAGTGAAACCAAGAGTAAAGTACTCGTTTTAGGCGGTTTAGGTTACATTGGGAGCTGTTTTATTGACCAGCTTCTAAAACAGTACCCTGATGTAACTGTTAGTGTAATTGACATTAACCACACTAGTCTGGCGCTACAACTGTTACCAAGACAGGTCAACGTTCACTTTGTTAACTTACTTGACCGTGCACAGTTAACAGATACGATTGCTCAAATTAACCCTGATGTCGTGTTTCACTTTGCGGCAAAAACAAGCGTAAAGGAATCGACCGAACAACCACTTACATATTTTGACCATAACCTAGTCGGTACGCTCAACTTACTCCACGCTTTAAAAGAACTACAAAAACCGATCCAGCTGTTCTTCTCCTCCACTGCTGCAGTCTTTGGTAGTGCTAGTACACTTCCAATTCCAGAAAACTTAGTTTTAGAGGAAACACTCGCTTCCAATCCGTATGGCATTAGTAAGTTTTTAAGTGAAATAGTACTGCAAACACTTACCCGTAGCCCCCATTTTCAAGTAATAGCGTTGCGCTACTTTAACGTAGCGGGTGCTAGTAATCCCTTTGGTAACTTCAATAAAAACACGACCTTACTGATTCCTAACCTGATTAAAGCCTTTATGGAAAAGCGCACCTTCTTTCTGTATGGTGATGACTATGACACTAAAGATGGGAGCTGTATCCGTGATTACATCCATGTAGTAGACTTGTGTGATGCCCATTTGTTGGCGTGA", "aa": "MSETKSKVLVLGGLGYIGSCFIDQLLKQYPDVTVSVIDINHTSLALQLLPRQVNVHFVNLLDRAQLTDTIAQINPDVVFHFAAKTSVKESTEQPLTYFDHNLVGTLNLLHALKELQKPIQLFFSSTAAVFGSASTLPIPENLVLEETLASNPYGISKFLSEIVLQTLTRSPHFQVIALRYFNVAGASNPFGNFNKNTTLLIPNLIKAFMEKRTFFLYGDDYDTKDGSCIRDYIHVVDLCDAHLLA", "aa_hexdigest": "1c4773f5a4ecc519cd36a8a96af491eb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557C9D", "query_cov": 0.8816326530612245, "subject_cov": 0.9432314410480349, "identity": 0.537, "score": 236.0, "evalue": 3.71e-75, "valid": false, "product": "NAD-dependent epimerase/dehydratase family protein", "uniref50_id": "UniRef50_UPI0000557C9D" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557C9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D" ], "product": "NAD-dependent epimerase/dehydratase family protein" }, "genes": [], "id": "CNOCLNGIAL_577", "locus": "GCA000143945_00542" }, { "type": "cds", "sequence": "contig_1", "start": 306026, "stop": 306298, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGCCAATCCTAAAGTGCGCTTTGAAAGCTTTAACTTAGGTAGTGGTCAGGGCTTTTCCAATTGGGAGGTCATTAATACTGCTCAAGCAATCTTTGCGCCTGAACAATTACAGCTCAAAATTGAATCACGAAAGGCTGGTGATCCACCTGTTTTAGTGGTTGATTGCACGAAGGCAAAACGCTTACTCAACTTCCAACCAACCCGCTCATTACACAAAATGCTGAGCGATGAAACAATTTTTTATCGGGATTTTTATAATAGGCTTTAA", "aa": "MQANPKVRFESFNLGSGQGFSNWEVINTAQAIFAPEQLQLKIESRKAGDPPVLVVDCTKAKRLLNFQPTRSLHKMLSDETIFYRDFYNRL", "aa_hexdigest": "916273d80b39269e01f30b5092e1fa9b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10362.752599999993, "isoelectric_point": 9.298935890197754 }, "id": "CNOCLNGIAL_578", "locus": "GCA000143945_00543" }, { "type": "cds", "sequence": "contig_1", "start": 306334, "stop": 307257, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030B82AEE" ], "nt": "ATGGAAAATATCTGCAAGAGTTTTGACAATGGTAGGGTCAAAGCTAATGTAGATGTTAACCTCACGGTTTACGAAAATACTGTCCACACATTGCTGGGTGAAAATGGTGCGGGCAAATCTACTTTAACTTCCATTTTGTTCGGTTTATACCAACCCGACAGCGGCAAAATTTTTATTGGTGAAGAGGAAGTACACTTCAAGTCCTCCAAGGATGCCGTGCAGCACAAAATTGGGATGGTGCACCAGCACTTTAAGCTGGTTGATAACTACACCGTCTTAGACAACATTATTTTGGGCAATGAAAGTAGTTTTTCCATTCCTTTTACCAACGGTAAGCTTAAACTACCTTTATTGCACCGTAAAGCTAGCGAAGCCAAAATTCAAGCAATGATGGAACGGTATGACCTCCATGTGAACTTGCACCAAAAGGTAAGCCGCTTAACGGTGGGTCAGCAACAACGGGTCGAGATCTTGAAGGTGTTATTCCGTGACAGTGACATTCTCATCTTTGATGAACCAACCGCTGTTTTGAGCGACCAGGAGATCAAGAGCTTTTTAAACATCATTAAAAACTTCAAGAAGATGGGTAAAACGATTGTGCTCATCTCCCACAAACTCAACGAAATTAAGGAAGTAGCGGAGACTGCTACCATTTTGCGTCAAGGTCACTCCGTTGGCACCTTCCAAATTAAGGACACTTCAATTGATGAAATGGCACGCTTAATGATGGGAAAGGAGTTGAAGAAAACCAAAAACAATACTCAGTTTACCGCCAAAGGTGAGCCCGTTTTAAAGGTCGAAAACCTCCATCTTTATTTAAACCAAAACTGGTTTTACAAGTTAATTGCGCGCTGGAACCAAAAGCGAATTAATCAACTCCAAAAACAGGGTAAGCCAGCGAAAACACTGTGGTTAAAGAGTTGA", "aa": "MENICKSFDNGRVKANVDVNLTVYENTVHTLLGENGAGKSTLTSILFGLYQPDSGKIFIGEEEVHFKSSKDAVQHKIGMVHQHFKLVDNYTVLDNIILGNESSFSIPFTNGKLKLPLLHRKASEAKIQAMMERYDLHVNLHQKVSRLTVGQQQRVEILKVLFRDSDILIFDEPTAVLSDQEIKSFLNIIKNFKKMGKTIVLISHKLNEIKEVAETATILRQGHSVGTFQIKDTSIDEMARLMMGKELKKTKNNTQFTAKGEPVLKVENLHLYLNQNWFYKLIARWNQKRINQLQKQGKPAKTLWLKS", "aa_hexdigest": "a8abefd1433abf18a4b202ec900ef133", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030B82AEE", "query_cov": 0.8664495114006515, "subject_cov": 0.9779411764705882, "identity": 0.508, "score": 243.0, "evalue": 1.77e-76, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI0030B82AEE" }, "pscc": { "uniref50_id": "UniRef50_UPI0030B82AEE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030B82AEE" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "CNOCLNGIAL_579", "locus": "GCA000143945_00544" }, { "type": "cds", "sequence": "contig_1", "start": 307267, "stop": 307635, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGGCAGCTATTGAAAAAACACCCCGTTTTATTCGTGGAATCGTTAATAATTTAGGTTTTGGTAGCCAACAGGTGTTTGATAAAGGGATTAGTTTTGAGATCCATAAGGGTGAAATTTTTGCCATTGCCGGGGTGGAAGGTAACGGTCAAAACCAATTAATAGATCTTATTTGTGGCTTGGAAAAAGCAGCACCAAAAAAGGTCTTCTTCAATGGTTTTGACATTTCACGTTATTCCATTCGCAAGCGGATTAACGCTGGTATTGGTTTTGTGTTAGAAGACCGCCATAAATACGGATTAATCTTAGACCAAACGGTGCGTTTTAACGCGGTGAATAACCAAATTGATCGTAAGCAATTTAGCAGTTGA", "aa": "MAAIEKTPRFIRGIVNNLGFGSQQVFDKGISFEIHKGEIFAIAGVEGNGQNQLIDLICGLEKAAPKKVFFNGFDISRYSIRKRINAGIGFVLEDRHKYGLILDQTVRFNAVNNQIDRKQFSS", "aa_hexdigest": "ed13da761070c5bc4fd52bc221665a26", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 82, "aa_cov": 0.6721311475409836, "hmm_cov": 0.9512195121951219, "evalue": 3.7890670964725056e-09, "score": 32.75098419189453, "start": 28, "stop": 109 } ], "seq_stats": { "molecular_weight": 13676.539799999979, "isoelectric_point": 9.607094764709473 }, "id": "CNOCLNGIAL_580", "locus": "GCA000143945_00545" }, { "type": "cds", "sequence": "contig_1", "start": 307651, "stop": 308031, "strand": "+", "frame": 1, "gene": "mglA", "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "nt": "ATGCAAATAGCTGTTTACACTAACCAAATCGTCGATAAATTTGATGTTCGTGGAGCGGTACAAGGTACAGCAATAGTGCGTTTACTTTCCGGGGGTAATCAGCAAAAGCTGATTATTGGTCGGGAACTGACAAAGCAGAACGAGCTGTTAGTGTTTGCTCAAGTAACCCGGGGTTTGGATGTTGGTGCCATTTCGTTTATCCACCAAAAGATTTTGGATGCAAAGAAACAAAATAATGCAATCTTGTTAGTGTCATATGAGTTAGATGAAATTTTAGCTATTGCCGATACCATTGGCGTAATTAACAAAGGCAACCTGTTGGAAGTCAATAAACGTGATGTAATGACGCGTGAACGCATAGGCAAACTAATCATGCAATAA", "aa": "MQIAVYTNQIVDKFDVRGAVQGTAIVRLLSGGNQQKLIIGRELTKQNELLVFAQVTRGLDVGAISFIHQKILDAKKQNNAILLVSYELDEILAIADTIGVINKGNLLEVNKRDVMTRERIGKLIMQ", "aa_hexdigest": "5ac53fec40fc3a7da533f40dc03ef0b4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M965", "query_cov": 0.9206349206349206, "subject_cov": 0.9354838709677419, "identity": 0.5429999999999999, "score": 126.0, "evalue": 4.14e-35, "valid": false, "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "uniref50_id": "UniRef50_A0A223M965" }, "pscc": { "uniref50_id": "UniRef50_A0A223M965", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "product": "Galactose/methyl galactoside import ATP-binding protein MglA" }, "genes": [ "mglA" ], "id": "CNOCLNGIAL_581", "locus": "GCA000143945_00546" }, { "type": "cds", "sequence": "contig_1", "start": 308048, "stop": 308704, "strand": "+", "frame": 2, "gene": "nupP", "product": "putative MG120-like protein", "db_xrefs": [ "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857", "SO:0001217", "UniRef:UniRef50_P75515", "UniRef:UniRef90_P75515" ], "nt": "ATGAATGCTTTAAATTACCTCAAACACCGTTTTCTGTTTAGTAAAGACAAGTTTTGGTACGCCCCTTTTAAACAAAAGCAACGTCGGAGCATCTACTCCACCTTTTCGTTGATTGTTCTCTCCTTTATTGTTTCGTTCTTTTTAATAGTCGCCATTCCCGGCATTAAAGGCGGTACCTTCTTAGAAATCTTTACCCGTTTATTTAAAGATAGGGTTAACATCGAAAACTTTGCACGACAAATTGCGATTTACACCCTAGCTGCTTTGGCGTTTAGCTTTTGTATGAGTGTTGGGGTGTTTAACATTGGGATTTCGGGACAAATGATGGCTGGCGCTAATTTTGGTTTCATGATGATCCTCAAGGTTTTCCCCGAGTCATTCCGTCCTGCCTTTGGTGGTCAGATCATTACCATCCTCTTAATGATTCTAGGTAGTGTGACCGTTGCTATGGTGGTAGCTGCTTTAAAGGTCTTCTTTAAGGTTAATGAAGTGGTTAGTGCCATTATGCTCAACTGGGTAATTGTGCTGGTTAGCGCTTATCTAGTGGGTACTTACATTAAACCGGAAAAAACTGATACTTCCCAGTTTTACTCGATTGAACTACCGGATGCCTTTGCGCTGTATAACTTCTCTGATGTTCAACAAAAGTATGGTTGA", "aa": "MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRVNIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYG", "aa_hexdigest": "64070d3705970c83c38547397748ff36", "start_type": "ATG", "rbs_motif": null, "genes": [ "nupP" ], "truncated": "3-prime", "pseudogene": { "start": 308048, "stop": 309007, "observations": { "mutation": [ 308702 ], "stop": [ 308702 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75515", "query_cov": 1.0, "subject_cov": 0.42166344294003866, "identity": 0.995, "score": 412.0, "evalue": 1.06e-140, "gene_start": 1, "gene_end": 218, "reference_start": 1, "reference_end": 218, "reference_sequence": "MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRINIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYGWLTSLVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYSVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNISKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQYWKQQLLVADVKRLTFKWDFLTFKHQQKYILRWYKGKNKKQTALENEFASLNEAISQKLEEK" }, "paralog": false, "identity": 0.9905956112852664, "score": 607.0, "evalue": 5.62e-220, "description": "Internal stop codon at 308702. Nonsense mutation around 308702.", "psc": { "uniref90_id": "UniRef90_P75515", "gene": "NupP", "product": "Uncharacterized protein MG120 homolog", "uniref50_id": "UniRef50_P75515", "cog_id": "COG4603", "cog_category": "F", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75515", "UniRef:UniRef50_P75515", "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P75515", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75515" ], "product": "Uncharacterized protein MG120 homolog" } }, "id": "CNOCLNGIAL_582", "locus": "GCA000143945_00547" }, { "type": "cds", "sequence": "contig_1", "start": 308714, "stop": 309418, "strand": "+", "frame": 2, "gene": "nupP", "product": "putative MG120-like protein", "db_xrefs": [ "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857", "SO:0001217", "UniRef:UniRef50_P75515", "UniRef:UniRef90_P75515" ], "nt": "TTGGTTATTGCCATAGCAGCTGCCATATTCATTGCAGTTTTAATGAAGTTCACCGTTTTTGGACACAAACTCAAGTCCACGGGTTTAAGTGTGACGGGTTCGCAAGCCGCTGGTTACATTGTTAAGAAGTATCAGTTCCTCTCGTTTGTGATTTCAGGAATCCTTTCTGGTTTACTAGCAGCTGTAGTTTATACCGCATCCTTTGAAAAGCAGTTAACGTTCTCAGATGTGGGTGACTTTGGGATTACATCGGTACCGATTACTGGGTTTGATGGAATTGCAATTGGTTTAATTGCGCTCAACAGTCCAGCGCGAATTGTAATAGTTTCCACCATTATTTCATTTGTAACCATTGGTGCTAAACCAGCGGGGTTAAACGCTGCTACTGCGAGCTTGGTGTTGGGTATCATGATGTATTTCGCTGCCATTTATAACCTGATGATTTACATCAAGCCATGGCGCATGATTGTCAAGCTCAATATTGGTAAGATGAACGAAGCGGCATATGATGAATTCCAAAACGAGATGGCTGCTAATTTAGAAACGCTGTCGTTCCAGCGCTTTTTAGATAAACAAAAACGGAAGCATGATAAAGAGCGAATGGTTTGGTTTGACACCAAGCGCTTTGAGGAGTACCAGAAAAAGAAGCAAGCAACTCTACAAACCTTCCATGAAAATTCCTCGCAAAACTTACTGCAGTACTGA", "aa": "MVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYIVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNIGKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQY", "aa_hexdigest": "8724749d3dbe60d121cef2da68e7e1ac", "start_type": "TTG", "rbs_motif": null, "genes": [ "nupP" ], "truncated": "both", "pseudogene": { "start": 308414, "stop": 309601, "observations": { "mutation": [ 308702, 309416, 309464 ], "stop": [ 308702, 309416, 309464 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75515", "query_cov": 1.0, "subject_cov": 0.4526112185686654, "identity": 0.987, "score": 427.0, "evalue": 1.24e-146, "gene_start": 1, "gene_end": 234, "reference_start": 223, "reference_end": 456, "reference_sequence": "MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRINIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYGWLTSLVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYSVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNISKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQYWKQQLLVADVKRLTFKWDFLTFKHQQKYILRWYKGKNKKQTALENEFASLNEAISQKLEEK" }, "paralog": false, "identity": 0.9873417721518988, "score": 748.0, "evalue": 4.54e-275, "description": "Internal stop codon at 308702, 309416, 309464. Nonsense mutation around 308702, 309416, 309464.", "psc": { "uniref90_id": "UniRef90_P75515", "gene": "NupP", "product": "Uncharacterized protein MG120 homolog", "uniref50_id": "UniRef50_P75515", "cog_id": "COG4603", "cog_category": "F", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75515", "UniRef:UniRef50_P75515", "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P75515", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75515" ], "product": "Uncharacterized protein MG120 homolog" } }, "id": "CNOCLNGIAL_583", "locus": "GCA000143945_00548" }, { "type": "cds", "sequence": "contig_1", "start": 309601, "stop": 309810, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACAGTCTTTAATTTCTTTTAGTAACTTAGATAACTGGTTGTTTGTCGCTCCTGCACTGCTACTCGCTGTATTGAGTGGTTACTTATCTGAGCGGGTTGGTATTGTCAATATTGCCATTAACGGGGGCATGGTGTTTGGCGGGATGTTCTTATCCTTAATGTCTTATGCCTTTGTGCCCAATGCAAATGATTCTGCACCATCTTGA", "aa": "MSQSLISFSNLDNWLFVAPALLLAVLSGYLSERVGIVNIAINGGMVFGGMFLSLMSYAFVPNANDSAPS", "aa_hexdigest": "c9258b06627954a98cba854935a5f624", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7300.407999999999, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_584", "locus": "GCA000143945_00549" }, { "type": "cds", "sequence": "contig_1", "start": 309904, "stop": 310335, "strand": "+", "frame": 1, "gene": "nupQ", "product": "putative MG121-like protein", "db_xrefs": [ "COG:COG1079", "COG:F", "GO:0005886", "GO:0022857", "KEGG:K23536", "SO:0001217", "UniRef:UniRef50_P47367", "UniRef:UniRef90_P75514" ], "nt": "GTGATTGTTGGCACAGGGGTTAACTTACTGGGCACGGGAATTAATTTCTTTGTGGCACAAAATGCCCGCTCATTGCTTAACGATACTGATTTAAGGGTACGATACAGCTTTGTAAGAACAGGAAACTCTGTAAGTATTGAGGGTATAGCAATCTTTGCCTTTGCAATTATCTTTGTTTTATTAGTGTGGTATTTGATGAACTTTACTAAAACGGGCTTGCGTTACCGTGCTGTTGGGGAAAATCCTAATGTGATTGATACCCAAGGTATTAGTGTGTATAAATACCAGTGGTTTGGGGTAATGGCTTCGACGATGGTAGCAGCTTTAGCGGGTTGTTGTTTTGCTTTAAGTCCACAGGTACCTAGCTTTTCTAGCGGGGATGTGAGCGGGTTTGGTTTTATTGCCATTGCCATTATGATCATTTCGATGTGA", "aa": "MIVGTGVNLLGTGINFFVAQNARSLLNDTDLRVRYSFVRTGNSVSIEGIAIFAFAIIFVLLVWYLMNFTKTGLRYRAVGENPNVIDTQGISVYKYQWFGVMASTMVAALAGCCFALSPQVPSFSSGDVSGFGFIAIAIMIISM", "aa_hexdigest": "acee666409cc5e121e0b09cfee537c26", "start_type": "GTG", "rbs_motif": null, "genes": [ "nupQ" ], "truncated": "both", "pseudogene": { "start": 309604, "stop": 310536, "observations": { "mutation": [ 309808, 310333 ], "stop": [ 309808, 310333 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75514", "query_cov": 1.0, "subject_cov": 0.45980707395498394, "identity": 0.993, "score": 278.0, "evalue": 4.11e-92, "gene_start": 1, "gene_end": 143, "reference_start": 102, "reference_end": 244, "reference_sequence": "MSQSLISFSNLDNWLFVAPALLLAVLSGYLSERVGIVNIAINGGMVFGGMFLSLMSYAFVPNANDSAPSWSLAISIPLSVIFASAVGFLFGIAAIKLKADHVIVGTGVNLLGTGINFFVAQNARSLLNDTDLRVRYSFVRTGNSVSIEGIAIFAFAIIFVLLVWYLMNFTKTGLRYRAVGENPNVIDTQGISVYKYQWFGVMASTMVAALAGCCFALSPQVPSFSSGDVSGFGFIAIAIMIISMWRIIPSIVISPLFALAYVLTTGVVGNANNTYLLRTIPFIISLLVMMVFGYLNVGPKNVGKHFDKGLR" }, "paralog": false, "identity": 0.9935483870967742, "score": 594.0, "evalue": 3.47e-219, "description": "Internal stop codon at 309808, 310333. Nonsense mutation around 309808, 310333.", "psc": { "uniref90_id": "UniRef90_P75514", "gene": "NupQ", "product": "Uncharacterized protein MG121 homolog", "uniref50_id": "UniRef50_P47367", "cog_id": "COG1079", "cog_category": "F", "kegg_orthology_id": "K23536", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75514", "UniRef:UniRef50_P47367", "COG:COG1079", "COG:F", "KEGG:K23536", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P47367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47367" ], "product": "Uncharacterized protein MG121" } }, "id": "CNOCLNGIAL_585", "locus": "GCA000143945_00550" }, { "type": "cds", "sequence": "contig_1", "start": 310354, "stop": 310536, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTCGCCCTTGTTTGCACTAGCTTATGTGCTTACTACCGGTGTGGTAGGTAATGCTAATAACACTTACTTATTGAGAACTATTCCGTTTATTATTTCGCTGTTGGTAATGATGGTATTTGGATATCTCAATGTAGGGCCGAAAAACGTTGGTAAGCATTTCGATAAGGGTTTGCGATAG", "aa": "MISPLFALAYVLTTGVVGNANNTYLLRTIPFIISLLVMMVFGYLNVGPKNVGKHFDKGLR", "aa_hexdigest": "c3c0b9758f28338dd47dce61416b593b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6599.869200000001, "isoelectric_point": 9.99467868804932 }, "id": "CNOCLNGIAL_586", "locus": "GCA000143945_00551" }, { "type": "cds", "sequence": "contig_1", "start": 310573, "stop": 310740, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAAATCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACGTTGCAAAAATATTTACCGAATGACTTTGAAATAGTTTCTACCATTGGTCATATTCGGGAAATGGTGCACAAAAACTTTGGTTTTAACGAAGCCGATTACTCACCAGTTTGGGAAGACTGA", "aa": "MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWED", "aa_hexdigest": "233f7614c4ddde2fe0020814a1fc0412", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6409.217500000001, "isoelectric_point": 5.53830051422119 }, "id": "CNOCLNGIAL_587", "locus": "GCA000143945_00552" }, { "type": "cds", "sequence": "contig_1", "start": 310924, "stop": 311205, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGAAAAGGACAAGTCGAAGTGCCAACGCATTACCTTTAACGAAATTACCAAGAACGCCGTTTTAGATGCACTGAAAAACCCACGGGAAATTGATCAGAGTTGGGTGCAGAGTCAGTTTGCGCGCCAAATTCTTGACCGGATGATTGGTTTTCGCCTTTCAAGGCTGTTAAACAATTACCTCTCAGCTAAGTCAGCGGGACGGGTACAATCAGTAGCACTGCGTTTTTTAGAAGAACGTGAACAGGAAATTCGTAGCTTTGTACCACGGTTTTGGTGA", "aa": "MDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFW", "aa_hexdigest": "86088825daf4df9ad4be62be951e9cf5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11062.560499999992, "isoelectric_point": 9.883535194396973 }, "id": "CNOCLNGIAL_588", "locus": "GCA000143945_00553" }, { "type": "cds", "sequence": "contig_1", "start": 311215, "stop": 312708, "strand": "+", "frame": 1, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0551", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_P47368", "UniRef:UniRef90_P78032" ], "nt": "GTGTTGTTAAATCCCAAGGCTGAAGGTGTTAGGGAGGCGTGTGCTAATCGTTCAATTCCAATTGTCTTGCGTGAAATTAATCCTGCTTTACGTGCTGGCTTGAAATTTGAAGAGGAGAAGAGCGTATCGGGAATTGACTTTTTAGATGAAGCATCAGCTAAAAAGTTTGGTGAACAGCTCAAGGGTGTCTTTGAGGTGTACAACATTGATGAAACCAAACACTACAGCAGTTCGCCCAATTCCGCTTACACCACCGCTTCATTGCAAAAGGATGCCATCAACAAACTAGGTTGGTCTTCCAAAAAGGTAACCTTAATTGCCCAACACCTGTATGAAGGGGTGAGTATTAATGGAGAACAAACCGCTTTAATTAGCTATCCCCGTACCGACTCAACTCGTTTATCAGCGCAATTTCAACAAAGTTGTAAAGAATACATCTTAAACCATTACGGAGAAAAGTACCTCTCTAATCGGATTGTAAGCGCTAAGGGAAAAAAAGGTGAAAAAATTATCCAAGACGCCCATGAAGCAATTCACCCAACTGACATGAACATTACCCCGGAAATGGTCAAGAATGCGATTAAGAAGGATGAGTTCTTACTGTACCGCTTAATCTGGATCCGTACGGTCGCTAGTTTAATGGCGGATTGTAAAAAATCACATACCCACATCCGGTTTATTAACGATGGTAATAAGTTTTACGCTTCTTCCAAGTCATTAGTATTTGATGGTTATCGCAAAATTTACGAACACTTTGAAAATAAGGAAAGTAATGATCTCTACATTGATTTGGATAAGATTAGGGTTGGTGATCGTTTCATGGCTAAAGACATTAAGATCACAGCCCGCCAAACCCATCCAGCAGCCCGTTATACCCAAGCTAGTTTAATTGAAGCGTTAGAAAAGTCCAATATTGGTCGACCTTCCACCTATAACACGATGGCTAGTGTTAATTTAGACCGGGGTTATGCTAGTTTAAATAAACATGCTTTTCACGTAACACAACTAGGGGAGCAGGTTAATGAAGAATTGTCCAATCACTTTGGTAAGATCATTAACAAAGAGTTCACTAAAAACATGGAGAAGTCTTTGGATGAAATTGCTGAAAATAAAAAGAACTACCAGGAGTTTCTCCGTGATTTTTGGTCAAACTTTAAGGAAGAGGTAAAGCTAGCGGAGGGTTCGATTCAAAGAGTGAAAAAGGAAAAGGAATTTGTTGGTCGTGATTGTCCAAGCTGTGCTAGTCCCTTGTTGTACCGCTATACTAAACGGGGTAATGAAAAGTTTGTTGGGTGCAGTAACTTTCCCAACTGTAAATACAACGAGTTTAGCCAAAATAAACCGAATTTAACTTTGGAAAAGCTCGAAGAACTGTGTCCGGAGTGTAACAGCCAACTAGTGAAAAGACGCACTAAGTTTAATCCCAATAAAACCTTTGTTGGTTGTAGTAATTTCCCTAGGTGCCGCTACATAAAGAAGGATAATGCTAGCTAG", "aa": "MLLNPKAEGVREACANRSIPIVLREINPALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLIAQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDMNITPEMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKIRVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSNHFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNEKFVGCSNFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS", "aa_hexdigest": "3a8769a4a47553b146f493d057c18262", "start_type": "GTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "5-prime", "pseudogene": { "start": 310915, "stop": 312708, "observations": { "mutation": [ 311203 ], "stop": [ 311203 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78032", "query_cov": 1.0, "subject_cov": 0.69901547116737, "identity": 0.97, "score": 927.0, "evalue": 0.0, "gene_start": 1, "gene_end": 497, "reference_start": 215, "reference_end": 711, "reference_sequence": "MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWEDWTKSKKKFSSLSFKGNLKGKKLLSKYDIIKSIKEKASKATNIYLATDPDREGEAISWHVYDVLDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFWWTLDVLLNPKAEGVREACANRSIPIVLREINPALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLIAQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINITPEMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKIRVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSKHFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNEKFVGCSNFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS" }, "paralog": false, "identity": 0.9748743718592965, "score": 1129.0, "evalue": 0.0, "description": "Internal stop codon at 311203. Nonsense mutation around 311203.", "psc": { "uniref90_id": "UniRef90_P78032", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_P47368", "cog_id": "COG0551", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78032", "EC:5.6.2.1", "UniRef:UniRef50_P47368", "COG:COG0551", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47368", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47368" ], "product": "DNA topoisomerase 1" } }, "id": "CNOCLNGIAL_589", "locus": "GCA000143945_00554" }, { "type": "cds", "sequence": "contig_1", "start": 312610, "stop": 312891, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTGTGCTTAATTTGTTCCGGCTCTAAGTGGTACTTTGTTCGTTTCTTGTTGTTTTTGAGGTGTAAATTTCAAGCAAAGAAAATGGTGATAATAAGCCAAATGAGGATACTTAAGATCAAAATGGCACTTACCAACGCTAAAATTATTGAGAAGGAAGTGGCACTTCTGCTCGTTAAACTAGCTAGCATTATCCTTCTTTATGTAGCGGCACCTAGGGAAATTACTACAACCAACAAAGGTTTTATTGGGATTAAACTTAGTGCGTCTTTTCACTAG", "aa": "MILCLICSGSKWYFVRFLLFLRCKFQAKKMVIISQMRILKIKMALTNAKIIEKEVALLLVKLASIILLYVAAPREITTTNKGFIGIKLSASFH", "aa_hexdigest": "c3a7f6b7d604f76d7baae66cb17fe82a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10606.091999999984, "isoelectric_point": 10.21915760040283 }, "id": "CNOCLNGIAL_590", "locus": "GCA000143945_00555" }, { "type": "cds", "sequence": "contig_1", "start": 312917, "stop": 313171, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGACTTTTACCAACAGCAGATTAACACTACTGCAACTGAGCTGAAGTGGTTGGATGGTCAGTTTCAGCAAATTGATGAAACCGATAAAAAGAAAGCGCATCAAATTACAATTAGACTAGCGCGGAACCAACTGTTACAACAGCTTAGTGTAAAGTTAGACCAAAAGCAGTTTAGTCAACGCGCTAATAATGAACTGCAAAAGTTAAAGTTGTCTAACTTAGAGAGCTTTACTGACCAAAAGATTAAATGA", "aa": "MLDFYQQQINTTATELKWLDGQFQQIDETDKKKAHQITIRLARNQLLQQLSVKLDQKQFSQRANNELQKLKLSNLESFTDQKIK", "aa_hexdigest": "1c9032a66d703ffe3a30586fcb0ff01c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9993.246099999993, "isoelectric_point": 9.346320152282715 }, "id": "CNOCLNGIAL_591", "locus": "GCA000143945_00556" }, { "type": "cds", "sequence": "contig_1", "start": 313349, "stop": 313648, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGACTGCAAGCAAAAAACCTGTTTAATAAGTTAATTGCTCAAAGTAGCAGTGTCAAGATGAGCGAAAAACTGATTAACAATGCTTTGCAATTAGTTCAGGACAATTTGGCATTACAAGCGAGTGAAAGTGGCAATAAGCTGCTCAAGGAGTTTGAATTATCGTGCACTAACACACAGCTAGTCCAACTGTTGGGCTTTCAGCAGTTTTACTTCGGTACAAACCTGCTTAGCTTACTTGATTTGAGCCGTTCAATCGCTGTTTTAGTGCGCTTTTTAAATGAGCACTGTAAGTGA", "aa": "MKRLQAKNLFNKLIAQSSSVKMSEKLINNALQLVQDNLALQASESGNKLLKEFELSCTNTQLVQLLGFQQFYFGTNLLSLLDLSRSIAVLVRFLNEHCK", "aa_hexdigest": "3b3dc50dad6cd8181041ef113159253d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11202.95829999999, "isoelectric_point": 9.342516517639162 }, "id": "CNOCLNGIAL_592", "locus": "GCA000143945_00557" }, { "type": "cds", "sequence": "contig_1", "start": 313670, "stop": 314125, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAACTGCTTTGTTTAACAACTTGCAATGGGTTAATAACAACGACTTCTTTTTAAAGTCACACAATGATTTAAAACAGCTGCACCTCTCCGCTGAACAGTTGGCGATTATTGAACAGCAAAACCGTCCCTTTTACATTGATGCTTATGCTTTATTGATTGCTGGCGTAAAGCAAATGCTGATGGAGCACGATGCTGTGGAACCAAAACAAATTCACTTTCATAATGCCAAAAAGGTAATGGAAAGCTTCCAGCTGTTTGGCATTGACCAGTTAGCGTTAATAGAGTACAACAACTGTCTCTATGGCTTTGTCACCACTAAACTCTATGAGATTAAACAATTAGATGATTTAGCTTTATTTAAAGTTTTGTTTAAAAGCTTTTTGAACAAGCATTTGAAACAAAAATTTGCAACAATCAGTTTATTTGTAAATACCCAAACACTCATGATCTAA", "aa": "METALFNNLQWVNNNDFFLKSHNDLKQLHLSAEQLAIIEQQNRPFYIDAYALLIAGVKQMLMEHDAVEPKQIHFHNAKKVMESFQLFGIDQLALIEYNNCLYGFVTTKLYEIKQLDDLALFKVLFKSFLNKHLKQKFATISLFVNTQTLMI", "aa_hexdigest": "2a1997b59ce9fb766075ff61232ee127", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17700.409200000002, "isoelectric_point": 6.579590797424316 }, "id": "CNOCLNGIAL_593", "locus": "GCA000143945_00558" }, { "type": "cds", "sequence": "contig_1", "start": 314329, "stop": 314499, "strand": "+", "frame": 1, "gene": "trxA", "product": "thioredoxin", "db_xrefs": [ "COG:COG3118", "COG:O", "GO:0005737", "GO:0015035", "SO:0001217", "UniRef:UniRef50_P47370", "UniRef:UniRef90_P75512" ], "nt": "GTGAGCACTGTAGCGTTTGCCAAGGTAAATGTCGATGAACAAACTGACATTGCCGCTGCTTACAAAATTACCTCACTACCTACAATTGTTTTATTTGAAAAAGGCCAGGAAAAGCACCGTGCTATTGGTTTTATGCCTAAAGCCAAGATCGTGCAACTTGTCAGCCAATAA", "aa": "MSTVAFAKVNVDEQTDIAAAYKITSLPTIVLFEKGQEKHRAIGFMPKAKIVQLVSQ", "aa_hexdigest": "96c9b5bd4ff0c3b29b76367e283794c2", "start_type": "GTG", "rbs_motif": null, "genes": [ "trxA" ], "truncated": "5-prime", "pseudogene": { "start": 314191, "stop": 314499, "observations": { "mutation": [ 314266 ], "stop": [ 314266 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75512", "query_cov": 1.0, "subject_cov": 0.5490196078431373, "identity": 0.982, "score": 107.0, "evalue": 5.32e-29, "gene_start": 1, "gene_end": 56, "reference_start": 47, "reference_end": 102, "reference_sequence": "MVTEIKSLKQLGELFASNNKVIIDFWAEWCGPCKITGPEFAKAASEVSTVAFAKVNVDEQTDIAAAYKITSLPTIVLFEKGQEKHRAIGFMPKAKIVQLVSQ" }, "paralog": false, "identity": 0.9901960784313726, "score": 198.0, "evalue": 2.23e-67, "description": "Internal stop codon at 314266. Nonsense mutation around 314266.", "psc": { "uniref90_id": "UniRef90_P75512", "gene": "trxA", "product": "thioredoxin", "uniref50_id": "UniRef50_P47370", "cog_id": "COG3118", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0015035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75512", "UniRef:UniRef50_P47370", "COG:COG3118", "COG:O", "GO:0005737", "GO:0015035" ] }, "pscc": { "uniref50_id": "UniRef50_P47370", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47370" ], "product": "Thioredoxin" } }, "id": "CNOCLNGIAL_594", "locus": "GCA000143945_00559" }, { "type": "cds", "sequence": "contig_1", "start": 314511, "stop": 314915, "strand": "+", "frame": 3, "gene": "cof", "product": "Putative phosphatase MPN_264", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "SO:0001217", "UniRef:UniRef50_P47371", "UniRef:UniRef90_P75511" ], "nt": "ATGATTGACTTGCTCGGGTTAGATCTTGATGGCACTTTGTTGTCTAGAACAAGGCAAATTAACGATCCTACTAAACAAGCTTTGGCTAATTTAATCCAAAAGAAGCCTAGCTTAAAGGTAATGATTCTAACCGGTAGATCATTATTCTCCACCTTGAAGTACGTTCAAGAGCTCAACGAGCTGTGCAAAAAACCGCTAGTGGAGTACTTTTGCTGTTATGGTGGGGCTAAGTTGTACCAACTCAATAACAACCAACCCCAAGAACAGTACAAGTTTTTAATTGACAGCCGGCAAGTTAAAACAGTGTTTGAAATTGTGGAACAACACAAGGGTTTGTTTTTGGCATACCTCGATAAACCAAAGGCACCCTACATTATTTTGGGGGCAAACCAGTTTTATGCTTGA", "aa": "MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYA", "aa_hexdigest": "5e427497861c4538a115bcba94e94620", "start_type": "ATG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 314511, "stop": 315218, "observations": { "mutation": [ 314913 ], "stop": [ 314913 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75511", "query_cov": 1.0, "subject_cov": 0.47686832740213525, "identity": 1.0, "score": 272.0, "evalue": 2.04e-90, "gene_start": 1, "gene_end": 134, "reference_start": 1, "reference_end": 134, "reference_sequence": "MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYAWLIKQFWYKQRCEYFKNDHLTDGILKINVYFACPLRLKKVYQIIKRQFQDTLNVVNFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN" }, "paralog": false, "identity": 0.9914893617021276, "score": 468.0, "evalue": 3.65e-170, "description": "Internal stop codon at 314913. Nonsense mutation around 314913.", "psc": { "uniref90_id": "UniRef90_P75511", "gene": "Cof", "product": "Putative phosphatase MPN_264", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P47371", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75511", "EC:3.1.3.-", "UniRef:UniRef50_P47371", "COG:COG0561", "COG:HR", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P47371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47371" ], "product": "Putative phosphatase MG125" } }, "id": "CNOCLNGIAL_595", "locus": "GCA000143945_00560" }, { "type": "cds", "sequence": "contig_1", "start": 314997, "stop": 315356, "strand": "+", "frame": 3, "gene": "cof", "product": "Putative phosphatase MPN_264", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "SO:0001217", "UniRef:UniRef50_P47371", "UniRef:UniRef90_P75511" ], "nt": "GTGTACTTCGCGTGTCCTTTAAGGTTGAAGAAGGTGTACCAGATCATTAAGCGTCAGTTTCAAGACACGCTTAACGTTGTGAGTTTTTCCAAGCACTTAATTGAAATTACCCACAAGGATGGAAATAAGGGCTATGCAATTGAAGCAATTGCCAAAAAACAAGGACTTTCGTTAAAACGGATGGCGGTGATTGGTGATTCCTTAAATGACCGTTCGATGTTTGAAAAGGTACAGTATTCGTTTGCAATGTCGAAATCACCCGATGAACTCAAACTGTTAGCCACTGAAATTGGCACTAAAACAAACCGCTTTCGTTTCAGTAGTTTAGTTGATTTAATTACCGAAAAAATTATTAACTAA", "aa": "MYFACPLRLKKVYQIIKRQFQDTLNVVSFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN", "aa_hexdigest": "69fb60fcf813f16bb097ff835c6cb12a", "start_type": "GTG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "5-prime", "pseudogene": { "start": 314697, "stop": 315356, "observations": { "mutation": [ 314913 ], "stop": [ 314913 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75511", "query_cov": 1.0, "subject_cov": 0.4234875444839858, "identity": 0.983, "score": 231.0, "evalue": 1.96e-74, "gene_start": 1, "gene_end": 119, "reference_start": 163, "reference_end": 281, "reference_sequence": "MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYAWLIKQFWYKQRCEYFKNDHLTDGILKINVYFACPLRLKKVYQIIKRQFQDTLNVVNFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN" }, "paralog": false, "identity": 0.9908675799086758, "score": 437.0, "evalue": 3.07e-158, "description": "Internal stop codon at 314913. Nonsense mutation around 314913.", "psc": { "uniref90_id": "UniRef90_P75511", "gene": "Cof", "product": "Putative phosphatase MPN_264", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P47371", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75511", "EC:3.1.3.-", "UniRef:UniRef50_P47371", "COG:COG0561", "COG:HR", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P47371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47371" ], "product": "Putative phosphatase MG125" } }, "id": "CNOCLNGIAL_596", "locus": "GCA000143945_00561" }, { "type": "cds", "sequence": "contig_1", "start": 315364, "stop": 316401, "strand": "-", "frame": 3, "gene": "trpS", "product": "tryptophan--tRNA ligase", "db_xrefs": [ "COG:COG0180", "COG:J", "EC:6.1.1.2", "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436", "SO:0001217", "UniParc:UPI0001DD650A", "UniRef:UniRef100_A0AB38W7A3", "UniRef:UniRef50_P75510", "UniRef:UniRef90_P75510" ], "nt": "ATGAAACGCGCTCTTACCGGGATTCAAGCATCGGGCAAACAGCATTTAGGCAACTATTTGGGGGTAATGCAGTCGTTAATTGAACTCCAAGAGCAATGCCAACTGTTTGTATTTGTCGCTGATTTACACTCAATTACCGTTGATTTTCAACCCCAAGCACTGAAACAAAATAACTTTGATTTGGTACGTACTTTATTAGCGGTTGGTCTAGATCCACAAAAAGCTTGTCTCTTTTTACAAAGCGATCTTTTGGAGCACAGTATGATGGGTTATTTAATGATGGTGCAAAGCAATCTCGGTGAACTGCAACGGATGACGCAGTTCAAGGCCAAAAAAGCGGAACAAACCCGTAACCCAAACGGCACCTTAAACATTCCCACCGGACTGTTAACTTATCCAGCCCTAATGGCGGGAGATATCTTGCTGTACCAACCTGACATTGTGCCCGTTGGTAATGACCAAAAACAACACCTAGAGCTTACTCGTGATTTAGCGCAGCGAATTCAAAAGAAGTTTAAGCTCAAGCTGCGTCTACCCCAGTTTGTCCAAAACAAAGATACCAACCGTATTATGGACTTATTTGACCCAACCAAAAAGATGAGTAAGTCCTCAAAGAACCAAAACGGAGTAATTTACTTGGATGACCCAAAAGAAGTAGTGGTTAAAAAAATTCGTCAAGCTACCACTGACAGCTTTAACAAAATCCGTTTTGCTTCCAAAACGCAGCCAGGGGTGACCAATATGCTCACAATTCTCAAGGCACTTTTAAAAGAGCCTGTCAATCAATCCTTAACCAATCAGTTAGGGAATGATCTAGAAGCTTACTTTAGTACTAAATCTTATTTAGATCTCAAAAACGCACTTACAGAAGCAACCGTTAATCTGCTAGTTAACATTCAGAGAAAACGGGAACAGATTAGTCGTGAACAGGTCTTTAACTGCCTCCAGGCGGGTAAAAATCAAGCCCAAGCAACGGCCAGAACAACACTAGCACTTTTCTATGATGGCTTTGGACTAGGAAGCCAAAACATCAAGTAA", "aa": "MKRALTGIQASGKQHLGNYLGVMQSLIELQEQCQLFVFVADLHSITVDFQPQALKQNNFDLVRTLLAVGLDPQKACLFLQSDLLEHSMMGYLMMVQSNLGELQRMTQFKAKKAEQTRNPNGTLNIPTGLLTYPALMAGDILLYQPDIVPVGNDQKQHLELTRDLAQRIQKKFKLKLRLPQFVQNKDTNRIMDLFDPTKKMSKSSKNQNGVIYLDDPKEVVVKKIRQATTDSFNKIRFASKTQPGVTNMLTILKALLKEPVNQSLTNQLGNDLEAYFSTKSYLDLKNALTEATVNLLVNIQRKREQISREQVFNCLQAGKNQAQATARTTLALFYDGFGLGSQNIK", "aa_hexdigest": "56a41ca3b949572a4723ff93bd46b54e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD650A", "uniref100_id": "UniRef100_A0AB38W7A3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD650A", "UniRef:UniRef100_A0AB38W7A3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7A3", "uniref90_id": "UniRef90_P75510" }, "psc": { "uniref90_id": "UniRef90_P75510", "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_P75510", "cog_id": "COG0180", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_P75510", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75510" ], "product": "Tryptophan--tRNA ligase" }, "genes": [ "trpS" ], "id": "CNOCLNGIAL_597", "locus": "GCA000143945_00562" }, { "type": "cds", "sequence": "contig_1", "start": 316461, "stop": 316898, "strand": "+", "frame": 3, "gene": "arsC", "product": "putative MG127-like protein", "db_xrefs": [ "COG:COG1393", "COG:P", "GO:0016491", "KEGG:K16509", "RefSeq:WP_010874623.1", "SO:0001217", "UniParc:UPI0000139303", "UniRef:UniRef100_P75509", "UniRef:UniRef50_P75509", "UniRef:UniRef90_P75509" ], "nt": "ATGCTTAAGAAAAAAGTTAATAATGATGCTGGTAAGACGTTTATCTTAATTTCATCATCTTGCTCGTCTTGCCAAAAGGCAATCGAATACTTTAAGGAAAATAATTTGAGTTATACGATCGAAAACTTTTACAAAAAACCGATAAGTGACAAGCGCTTTCGTGACATTTTAAGTTTGAGTGAAGATGGCACCGAAAGTCTGTTTTCCAAGCGTGCTGACCAAATTCGTAGTAACACTAATCAATCAGTGGAAGATTTGACCATTCCTGAATTGATTAAGTTAATTCGCGAACGTCCTTCAATTTTGCGTCGACCAATTATTATCCAGTACAACTCTTCGGGGATTCCCAAGCGGATGCGCATTGGTTATAACGCCGCTGAAATTAAGGTATTTGAACGCAGCTTAATGGAACCTAAAGTGCGTACAGTAAAGAAGTAA", "aa": "MLKKKVNNDAGKTFILISSSCSSCQKAIEYFKENNLSYTIENFYKKPISDKRFRDILSLSEDGTESLFSKRADQIRSNTNQSVEDLTIPELIKLIRERPSILRRPIIIQYNSSGIPKRMRIGYNAAEIKVFERSLMEPKVRTVKK", "aa_hexdigest": "f928a8bde45f22565b6347623ee5e9b0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139303", "ncbi_nrp_id": "WP_010874623.1", "uniref100_id": "UniRef100_P75509", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139303", "RefSeq:WP_010874623.1", "UniRef:UniRef100_P75509" ] }, "ips": { "uniref100_id": "UniRef100_P75509", "uniref90_id": "UniRef90_P75509" }, "psc": { "uniref90_id": "UniRef90_P75509", "gene": "ArsC", "product": "Uncharacterized protein MG127 homolog", "uniref50_id": "UniRef50_P75509", "cog_id": "COG1393", "cog_category": "P", "kegg_orthology_id": "K16509", "go_ids": [ "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75509", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75509" ], "product": "Uncharacterized protein MG127 homolog" }, "genes": [ "arsC" ], "id": "CNOCLNGIAL_598", "locus": "GCA000143945_00563" }, { "type": "cds", "sequence": "contig_1", "start": 316898, "stop": 317677, "strand": "+", "frame": 2, "gene": "nadK", "product": "NAD(+) kinase", "db_xrefs": [ "COG:COG0061", "COG:H", "EC:2.7.1.23", "GO:0003951", "GO:0005524", "GO:0005737", "GO:0006741", "GO:0019674", "GO:0046872", "GO:0051287", "KEGG:K00858", "RefSeq:WP_010874624.1", "SO:0001217", "UniParc:UPI00001320B9", "UniRef:UniRef100_P75508", "UniRef:UniRef50_P75508", "UniRef:UniRef90_P75508" ], "nt": "ATGAAGTACAAGATTTTTGCATCAACAACACCACAAACCGAGCCAGTTTTGCAAAAGTTAAAGCAGGTGTTGAAAGGTTGTGAGGCAGTTGAAAAGGGCTTTGATTATCTCTTTGTATTAGGTGGTGATGGTTTCTTTGTTTCTACTGTGGCCAACTATAACTGCCACAATTGTAGGGTAGTAGGGATTAACACTGGTCATCTAGGTTTTTACACATCGTTTAATGAAAAGGATTTGGATGATAATTTCCTCCAAAAGCTCCAGCAATGTCACTTTCAACGGATTAGTTTGTTGGAAGTTAGTGTCAATGGCCAGCAGCACTTGGTTTTGAATGAACTGGCAGTTTACACCAACACAGCTTACCCAATTAACATCTTTATTGATGGTGAAGCGTGGGAGTTTTACCGCGGTTCAGGTTTATTAATCGGTCCGCGTACTGGTTCGACTGCACTGGCTAAATCTGCTAAAGGGGCGGTGATTTTCCCTGGCATTGATGTGCTGCAAATTATTGAAATGAATCCACTTTTGCACCCCAATCAAGTTACTATCCAATCACCAATTATTTTGCCCAAAGAAACGCAAGTGGAATTTGTGGTCAAAAAAGCCTTTAACCCACAACAGTTTCCCACCTTTTACTGTGACGGGAGAAAGTTGGAATTGCCAAACGCTGACACAACTTTAGCGCTTAAACTAGTGCAATCCACACCGATGTTTAACATTTCACTCAAAACACAGGACTTTATCAATAAACTTAAGTCCACTTTTATTAAACAAAGCTAA", "aa": "MKYKIFASTTPQTEPVLQKLKQVLKGCEAVEKGFDYLFVLGGDGFFVSTVANYNCHNCRVVGINTGHLGFYTSFNEKDLDDNFLQKLQQCHFQRISLLEVSVNGQQHLVLNELAVYTNTAYPINIFIDGEAWEFYRGSGLLIGPRTGSTALAKSAKGAVIFPGIDVLQIIEMNPLLHPNQVTIQSPIILPKETQVEFVVKKAFNPQQFPTFYCDGRKLELPNADTTLALKLVQSTPMFNISLKTQDFINKLKSTFIKQS", "aa_hexdigest": "2a76f8b4329ae23c917b427157968987", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001320B9", "ncbi_nrp_id": "WP_010874624.1", "uniref100_id": "UniRef100_P75508", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001320B9", "RefSeq:WP_010874624.1", "UniRef:UniRef100_P75508" ] }, "ips": { "uniref100_id": "UniRef100_P75508", "uniref90_id": "UniRef90_P75508" }, "psc": { "uniref90_id": "UniRef90_P75508", "gene": "nadK", "product": "NAD(+) kinase", "ec_ids": [ "2.7.1.23" ], "uniref50_id": "UniRef50_P75508", "cog_id": "COG0061", "cog_category": "H", "kegg_orthology_id": "K00858", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ] }, "pscc": { "uniref50_id": "UniRef50_P75508", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75508" ], "product": "NAD kinase" }, "genes": [ "nadK" ], "id": "CNOCLNGIAL_599", "locus": "GCA000143945_00564" }, { "type": "cds", "sequence": "contig_1", "start": 317704, "stop": 318030, "strand": "+", "frame": 1, "gene": "ptsG2", "product": "Putative phosphotransferase enzyme IIB component MPN_268", "db_xrefs": [ "COG:COG1264", "COG:G", "EC:2.7.1.-", "GO:0008982", "GO:0009401", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75507", "UniRef:UniRef90_P75507" ], "nt": "GTGTTGAGCTTTGGCTTACTTTACCTTTATTTGGTAAAACGTGCTAAGAGGGCCGCGCTACAATTAAACAACAAACTAGTCGAATCACACACAATTCCTTTTGCTGTACGTGACTTTATTGCCGCTTGCGGGGGTAGAACTAACTTTGTTAGTTTACGCACCTCACCCACCCAATTAATTGTTAGTTTTGCCAAGCCAGAGTTAGTTCAAATTGCCGCTTTGCAAAAGCTTGGAATTAAAGGGATTAATAAGAGTCAGAACCAGTACCGCTTTGTATTGGGCAATTTTGTCAATCAATTAAAACAGCAAATTGAAAATGAGCGCTAA", "aa": "MLSFGLLYLYLVKRAKRAALQLNNKLVESHTIPFAVRDFIAACGGRTNFVSLRTSPTQLIVSFAKPELVQIAALQKLGIKGINKSQNQYRFVLGNFVNQLKQQIENER", "aa_hexdigest": "13d04c6ff2e45ac7835b0b3ce2bab351", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75507", "query_cov": 0.9907407407407407, "subject_cov": 0.9145299145299145, "identity": 1.0, "score": 204.0, "evalue": 1.39e-66, "valid": true, "gene": "PtsG2", "product": "Putative phosphotransferase enzyme IIB component MPN_268", "ec_ids": [ "2.7.1.-" ], "uniref50_id": "UniRef50_P75507", "cog_id": "COG1264", "cog_category": "G", "go_ids": [ "GO:0016020", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75507", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75507" ], "product": "Putative phosphotransferase enzyme IIB component MPN_268" }, "genes": [ "ptsG2" ], "id": "CNOCLNGIAL_600", "locus": "GCA000143945_00565" }, { "type": "cds", "sequence": "contig_1", "start": 318020, "stop": 319333, "strand": "+", "frame": 2, "gene": "rny", "product": "Ribonuclease Y", "db_xrefs": [ "COG:COG1418", "COG:JR", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0005886", "GO:0006402", "SO:0001217", "UniRef:UniRef50_P47376", "UniRef:UniRef90_P75506" ], "nt": "ATGAGCGCTAAGTTAACGTTAGAAAGCATTGCAAAAACATTTGCCGAAACATCGATCTTTGCGATCTTGTTTTTAATTATTGTCATCCTCAATTTAGGTTTACTTGTTTTTTTAGCGTATCAGTACCGTGTTTACAAAAAGAAGCAAAGAGCAAACCTCACCAAGCAGACTTATAACAATGACTATGGCCAAATTGTTAATTTAAAACAGCAAAACGCTGCCAAGATAAAGGAGCTTGCTAACTTAAAAGATCAGTTAAGTGAATTAGGCCAAAAATTTAACACCACACTGAGTGAAATCATTAACAAACCACTTGTCAATGTGATTGATGAATACTTGGATGAACAGTTTAAACAAGCTGCTAATTTTCGTGAAGCAGAACTTAATGCCGTTTTAGATAGTAATGACCAGAAAACAGTGTTCCATAAGCGTTTGTTCAATAAATTTCACTTTGGTGTCGACAAGCTAGCTAATATTAATGTCAAAAATCCGCTCAATTTATGCTGGGTTGACAGCGCTAGTTTTACCGTGATTGAAAGTGATTTCCGCAAACTTAATGGTGTTGGCGGGATTAATAAAAAGTTGTTAATTGAAAAGTTAAGGATTGAAGATATTATCTTTACCAATATCGATAAGAAGTACTATGAGGTACAGATACTATCTGACTCGCCAGTAAAGGTACAAAAAACAGTGTTAACTATTCGCAACATCTTGATTAATGATTACGTGGACAATGAAAAAATTGAGTCATATGCTAGGGAAGCTAACGGTTACTTCAATGACCACTGTAAACTGATTGGTAAGCAGGTGTTAGAACGACTCAATATCTTTGAAATTAGCCCTAAACTACACAAGTTTTTTGGTTTATTAGCGTTTCGTTATTCTTTCGGTCAAAACGTTTTATCACACTGTCTGGAAACTGGTTTTCTCACCGCTTACCTAGCGCTTTTAGTTAACTTTAAACCTGATGTTGCATTAAAGTGCGGTTTGTATCATGATATTGGTAAAGCGGATGATGAAAACGGGAAGAAGAACCACACGGTTACAGGTGCCAAGATCGGTGATGAATTTTACTTTGAAAACGATGTCAAATACACAATTGCCAACCACCATAATAAAAATGTCGATAACGTATACTGTCGTTTAACTCAAATTGGCGATAAGCTTTCAGCAGGGCGTTTGGGTGCGCGCTCTGACAGTTCGGTTTTATTTAACCAACTCAAACAAGAATTAAAGCAAATTGTGGAAGAAACACTAGCACAATTTAAAACAACCATCCTTTTAGGACAAAGTGGACGCCGATTAGTAATTTGA", "aa": "MSAKLTLESIAKTFAETSIFAILFLIIVILNLGLLVFLAYQYRVYKKKQRANLTKQTYNNDYGQIVNLKQQNAAKIKELANLKDQLSELGQKFNTTLSEIINKPLVNVIDEYLDEQFKQAANFREAELNAVLDSNDQKTVFHKRLFNKFHFGVDKLANINVKNPLNLCWVDSASFTVIESDFRKLNGVGGINKKLLIEKLRIEDIIFTNIDKKYYEVQILSDSPVKVQKTVLTIRNILINDYVDNEKIESYAREANGYFNDHCKLIGKQVLERLNIFEISPKLHKFFGLLAFRYSFGQNVLSHCLETGFLTAYLALLVNFKPDVALKCGLYHDIGKADDENGKKNHTVTGAKIGDEFYFENDVKYTIANHHNKNVDNVYCRLTQIGDKLSAGRLGARSDSSVLFNQLKQELKQIVEETLAQFKTTILLGQSGRRLVI", "aa_hexdigest": "65ed0af05d56a202e97a13a87d3974d9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75506", "query_cov": 1.0091533180778032, "subject_cov": 0.8873239436619719, "identity": 0.975, "score": 825.0, "evalue": 2.37e-300, "valid": true, "gene": "rny", "product": "Ribonuclease Y", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47376", "cog_id": "COG1418", "cog_category": "JR", "go_ids": [ "GO:0005886", "GO:0003723", "GO:0004521", "GO:0006402" ] }, "pscc": { "uniref50_id": "UniRef50_P47376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47376" ], "product": "Ribonuclease Y" }, "genes": [ "rny" ], "id": "CNOCLNGIAL_601", "locus": "GCA000143945_00566" }, { "type": "cds", "sequence": "contig_1", "start": 319334, "stop": 319501, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACCAACCAGCACAATAACATTATTGACAACCAACAGTTAACTGATTTGGCTACTACCATTAAAAGCAAAATCGTGCAAAACAACATTACTAATCGCTTTCCAATTAAGGTAGTATTACGCTATAACTTCGAACACAGCTTTGATACAAAGGACAAGAACTAG", "aa": "METNQHNNIIDNQQLTDLATTIKSKIVQNNITNRFPIKVVLRYNFEHSFDTKDKN", "aa_hexdigest": "a8d39b1aa755cf66f792235a554d7531", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6504.217700000001, "isoelectric_point": 8.140245628356933 }, "id": "CNOCLNGIAL_602", "locus": "GCA000143945_00567" }, { "type": "cds", "sequence": "contig_1", "start": 319504, "stop": 319791, "strand": "+", "frame": 1, "gene": null, "product": "putative MG131-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874627.1", "SO:0001217", "UniParc:UPI000013931E", "UniRef:UniRef100_Q9EXD1", "UniRef:UniRef50_Q9EXD1", "UniRef:UniRef90_Q9EXD1" ], "nt": "ATGCAATACACCGTTTTAATTCCACTGTTTATTTTTATAGGAGCCATGGTGTTGTTTGGTTTTAGCTTTCAGAAAAAGCAACCACAACGCCGCATCGTTCAAATTCTGTTCTTAGCTTACTGTGTGGATTTTTTAGCACTAATTATTGCAGTGATGATGCTCACATTCTTAAGTTATGATGACTTAATGCTGGGCGTTTTAATTCCAGTATTAATCCTTTCAATCATTATGTTCTTTGTGATGATTATTGCCCACTATCCACTAATGAAACGCTTGTTTGGACATTAG", "aa": "MQYTVLIPLFIFIGAMVLFGFSFQKKQPQRRIVQILFLAYCVDFLALIIAVMMLTFLSYDDLMLGVLIPVLILSIIMFFVMIIAHYPLMKRLFGH", "aa_hexdigest": "c68edcf8234a323dcf7d6b19cf7a548a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013931E", "ncbi_nrp_id": "WP_010874627.1", "uniref100_id": "UniRef100_Q9EXD1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013931E", "RefSeq:WP_010874627.1", "UniRef:UniRef100_Q9EXD1" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD1", "uniref90_id": "UniRef90_Q9EXD1" }, "psc": { "uniref90_id": "UniRef90_Q9EXD1", "product": "Uncharacterized protein MG131 homolog", "uniref50_id": "UniRef50_Q9EXD1", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD1" ], "product": "Uncharacterized protein MG131 homolog" }, "genes": [], "id": "CNOCLNGIAL_603", "locus": "GCA000143945_00568" }, { "type": "cds", "sequence": "contig_1", "start": 320037, "stop": 320231, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTTTGCCTTCTTTAGTTTTAATTGCTTTAGACAAATCAATGTCTTTACCAGTGGTTTCTTTAATAAGATTTCTAAGATCAGCTTGGATACCAGTGCAAGCACTTAACAATGTTCCACATAATAAAAATAGAGAACTAAAAGAAAACCTTGACAAAAACTTCTTTTTACGCATTAACAGTAAAAAATAA", "aa": "MFFLPSLVLIALDKSMSLPVVSLIRFLRSAWIPVQALNNVPHNKNRELKENLDKNFFLRINSKK", "aa_hexdigest": "fb79d38aa40892fca75e530f2cfb7137", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7465.829599999998, "isoelectric_point": 10.620408821105958 }, "id": "CNOCLNGIAL_604", "locus": "GCA000143945_00569" }, { "type": "cds", "sequence": "contig_1", "start": 320388, "stop": 320477, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGGTTGATCATTTTCAAATGACATACCAAAGTTTTAAGGATTTGTTAGTTGAGGCTAGGCTGAGCTATACCTTTAATTGA", "aa": "MERKVDHFQMTYQSFKDLLVEARLSYTFN", "aa_hexdigest": "e34c401a42d6c8923a0331cea5fd4f57", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3598.0693, "isoelectric_point": 6.518261528015137 }, "id": "CNOCLNGIAL_605", "locus": "GCA000143945_00570" }, { "type": "cds", "sequence": "contig_1", "start": 320497, "stop": 320649, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTCACTGCTAAGAGAGGTGACAAGCATTATTTTTATCTTTTCCTTAAAATTAAATCTGATCCAAAAAAACAATTTTCAGCAAAAAAATTTCTTACTGAAGGAGAAGCGTTTACTGATCAAGAAGGAAAACAAACTACAAGAAATTTAG", "aa": "MISLLREVTSIIFIFSLKLNLIQKNNFQQKNFLLKEKRLLIKKENKLQEI", "aa_hexdigest": "fff8c858299082528508dd4a4a4b764c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6060.288099999998, "isoelectric_point": 10.214515876770019 }, "id": "CNOCLNGIAL_606", "locus": "GCA000143945_00571" }, { "type": "cds", "sequence": "contig_1", "start": 321826, "stop": 322077, "strand": "-", "frame": 3, "gene": null, "product": "Restriction-modification enzyme subunit S1B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W825", "UniRef:UniRef90_A0AB38W825" ], "nt": "TTGCAAACATCTAAGTTTGTTAATTATGCTTGCCCTATTCCTAAATTAGGAATAAGTAAATTAGCAGAAATTGAACTTACTTTCCCACCTCTAGAAATTCAAGAAAAGATTGCCGATATCCTCTTTGCTTTTGAAAAAAATGTAACGATCTTACCGAAAGTATTCCCAAAGAAATTGAAATGCGTAAAAAGCAATTGGATTATTACCAAAACTATTTATTCGATTGAGTTCAAAAACAAAAAGAAGGCTTAA", "aa": "MQTSKFVNYACPIPKLGISKLAEIELTFPPLEIQEKIADILFAFEKNVTILPKVFPKKLKCVKSNWIITKTIYSIEFKNKKKA", "aa_hexdigest": "6b9dc874c7682bf42e295363c3f2e7a2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 321826, "stop": 322269, "observations": { "mutation": [ 322209 ], "stop": [ 322209 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W825", "query_cov": 1.0, "subject_cov": 0.564625850340136, "identity": 0.988, "score": 166.0, "evalue": 2.25e-51, "gene_start": 1, "gene_end": 83, "reference_start": 65, "reference_end": 147, "reference_sequence": "MNDGEVGKIKDCDRDGEYVTWTTGGSYAGAVFYRKGQFNARQDCGVLKVKNEEINPKFLAYALRLQTSKFVNYACPIPKLGISKLAEIELTFPPLEIQEKIADILFAFEKNVTILPKVFPKKLKCVKSNWIITKTIYSIEFKNKKKA" }, "paralog": false, "identity": 0.9863945578231292, "score": 295.0, "evalue": 1.36e-104, "description": "Internal stop codon at 322209. Nonsense mutation around 322209.", "psc": { "uniref90_id": "UniRef90_A0AB38W825", "product": "Restriction-modification enzyme subunit S1B", "uniref50_id": "UniRef50_A0AB38W825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W825", "UniRef:UniRef50_A0AB38W825" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W825" ], "product": "Restriction-modification enzyme subunit S1B" } }, "id": "CNOCLNGIAL_607", "locus": "GCA000143945_00572" }, { "type": "cds", "sequence": "contig_1", "start": 322513, "stop": 322716, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_272", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_Q9EXD2", "UniRef:UniRef90_Q9EXD2" ], "nt": "ATGAACCGTCCTACTCCCAATTTTGAAGCAATCGATAAGAAGATTAGTGCCTTTGTCACTAACCACGATAACTTATTGGATAAGTTACTCAAACAACAAACCGAATTGTTAACTAGTGAAATTACGACTAATTTTGAGGTAACCCAACAAATCCAGGAAGAAGTAGCCAAAAAGACGAAGCAACACTCTAAAAACTACAAGTGA", "aa": "MNRPTPNFEAIDKKISAFVTNHDNLLDKLLKQQTELLTSEITTNFEVTQQIQEEVAKKTKQHSKNYK", "aa_hexdigest": "669edc04f61a0ea699ba98706a6e0463", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 322513, "stop": 322794, "observations": { "mutation": [ 322714 ], "stop": [ 322714 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9EXD2", "query_cov": 1.0, "subject_cov": 0.7204301075268817, "identity": 1.0, "score": 128.0, "evalue": 1.89e-37, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MNRPTPNFEAIDKKISAFVTNHDNLLDKLLKQQTELLTSEITTNFEVTQQIQEEVAKKTKQHSKNYKWLVTVVLANGVVSLFLLGGLIYLFSK" }, "paralog": false, "identity": 0.978494623655914, "score": 177.0, "evalue": 3.68e-59, "description": "Internal stop codon at 322714. Nonsense mutation around 322714.", "psc": { "uniref90_id": "UniRef90_Q9EXD2", "product": "Uncharacterized protein MPN_272", "uniref50_id": "UniRef50_Q9EXD2", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9EXD2", "UniRef:UniRef50_Q9EXD2", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD2" ], "product": "Uncharacterized protein MPN_272" } }, "id": "CNOCLNGIAL_608", "locus": "GCA000143945_00573" }, { "type": "cds", "sequence": "contig_1", "start": 322771, "stop": 323205, "strand": "-", "frame": 3, "gene": "hinT", "product": "putative HIT-like protein", "db_xrefs": [ "COG:COG0537", "COG:FR", "GO:0003824", "GO:0009117", "RefSeq:WP_014325433.1", "SO:0001217", "UniParc:UPI0001DD650D", "UniRef:UniRef100_A0AAX0SPP5", "UniRef:UniRef50_P47378", "UniRef:UniRef90_P75504" ], "nt": "ATGGTGCAAAAACAAAGTATGGCAAACAATAACTGTATCTTCTGTGGAATTGTAGAGGGGAATGTGAAATCCTTTAAGGTTGGTGAAAACGAACACGCTGTTGCCTTTTTAGATGCCTTTCCAGTTGCTGATGGTCATACGCTTGTAATTCCCAAAAAACACGCCGTTAACTATTCCTCGACTGATGATGAGAGTTTGAAGGCGGTTAGTTTACTCGCCAAAGAAATGGCTTTAAAGCTACAACAACGGCTCCAACCAGCTGGATTAAACTATGTTGTCAATGAAGGGGCTAAGGCAGGGCAGGAAGTATTTCACTACCACATGCATGTAGTACCAAAATACGAAACTGGTTTAGGCTTTTGTTACAACGTTAAAAAGACGAACAACCGCTCAATTGAAGCTAATTGGGAACTACTTACTAAAGAGGTAGATTAA", "aa": "MVQKQSMANNNCIFCGIVEGNVKSFKVGENEHAVAFLDAFPVADGHTLVIPKKHAVNYSSTDDESLKAVSLLAKEMALKLQQRLQPAGLNYVVNEGAKAGQEVFHYHMHVVPKYETGLGFCYNVKKTNNRSIEANWELLTKEVD", "aa_hexdigest": "4b99a9b8fd1a3154440d371ca496d6d7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD650D", "ncbi_nrp_id": "WP_014325433.1", "uniref100_id": "UniRef100_A0AAX0SPP5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD650D", "RefSeq:WP_014325433.1", "UniRef:UniRef100_A0AAX0SPP5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SPP5", "uniref90_id": "UniRef90_P75504" }, "psc": { "uniref90_id": "UniRef90_P75504", "gene": "HinT", "product": "Uncharacterized 16.1 kDa HIT-like protein", "uniref50_id": "UniRef50_P47378", "cog_id": "COG0537", "cog_category": "FR", "go_ids": [ "GO:0003824", "GO:0009117" ] }, "pscc": { "uniref50_id": "UniRef50_P47378", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47378" ], "product": "Uncharacterized HIT-like protein MG132" }, "genes": [ "hinT" ], "id": "CNOCLNGIAL_609", "locus": "GCA000143945_00574" }, { "type": "cds", "sequence": "contig_1", "start": 323273, "stop": 323452, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCGAGCCGCTACATGAATTGGGTAAGTTTTTACTCAAGGCAGGGGTGTACCCAAGCCTTTATGCCTTTTATTTACAAACAGTACCTTTTCTAAAGATATGGGACAACGGGCATGATTCGTACTCGGTCTATGGTTTCTTCTCCCAAACCAAATATAACAGCTATGTTTGGTTTTGA", "aa": "MREPLHELGKFLLKAGVYPSLYAFYLQTVPFLKIWDNGHDSYSVYGFFSQTKYNSYVWF", "aa_hexdigest": "6600a0c7effaa00232f4fb117449c3e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7063.991900000001, "isoelectric_point": 8.003379249572756 }, "id": "CNOCLNGIAL_610", "locus": "GCA000143945_00575" }, { "type": "cds", "sequence": "contig_1", "start": 323537, "stop": 323974, "strand": "-", "frame": 2, "gene": null, "product": "putative MG133-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47379", "UniRef:UniRef90_P75503" ], "nt": "ATGAAAAAAACAATAGGACTCGCTTACCGTTTTTTCTATCTTAACAATAATTGCGACTTTTATTTACTTTTTCTCGCGCCGTTTTCCCTGTTTAACCTTGGGGTAATGGTAGCTAGTGCTGTTATCAGTGTGGTTTATAACAACCAACCACAATTAATCTGGTTTACTAACTTTGATACCTTTACCTACCAGAGCAACACAATTGCCGCTGTTTGTGTCTTAATGTACCTCTGTAAACGACGCTGTAAGCTGTTTGACAATAATGCGTTGTTTTTAAGTGCTGCAGGTTACTTGGTCTTTACCGTCATCTTTTTTAACCTGTATGTACTGTCACGTGTTACTGGATTTGTTAATGTAGAAGAACACGTTAAGGGTTGGTTTTCGACTATTACGAGTGAAATGCCATACAGTTTTAGTGGTAATCCTTTAACTGATTGA", "aa": "MKKTIGLAYRFFYLNNNCDFYLLFLAPFSLFNLGVMVASAVISVVYNNQPQLIWFTNFDTFTYQSNTIAAVCVLMYLCKRRCKLFDNNALFLSAAGYLVFTVIFFNLYVLSRVTGFVNVEEHVKGWFSTITSEMPYSFSGNPLTD", "aa_hexdigest": "169616fd36fea1a5825ef43ee09f50b5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 323234, "stop": 323974, "observations": { "mutation": [ 323275, 323476, 323539 ], "stop": [ 323275, 323476, 323539 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75503", "query_cov": 1.0, "subject_cov": 0.5451127819548872, "identity": 1.0, "score": 293.0, "evalue": 5.94e-99, "gene_start": 1, "gene_end": 145, "reference_start": 1, "reference_end": 145, "reference_sequence": "MKKTIGLAYRFFYLNNNCDFYLLFLAPFSLFNLGVMVASAVISVVYNNQPQLIWFTNFDTFTYQSNTIAAVCVLMYLCKRRCKLFDNNALFLSAAGYLVFTVIFFNLYVLSRVTGFVNVEEHVKGWFSTITSEMPYSFSGNPLTDWISFAQLFLHVIYPASFFGFIWIFFKTYKMREPLHELGKFLLKAGVYPSLYAFYLQTVPFLKIWDNGHDSYSVYGFFSQTKYNSYVWFWSIPIFASMFLILWGLFVINNRYYGAKTKYGKQ" }, "paralog": false, "identity": 0.9878048780487805, "score": 497.0, "evalue": 1.75e-181, "description": "Internal stop codon at 323275, 323476, 323539. Nonsense mutation around 323275, 323476, 323539.", "psc": { "uniref90_id": "UniRef90_P75503", "product": "Uncharacterized protein MG133 homolog", "uniref50_id": "UniRef50_P47379", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75503", "UniRef:UniRef50_P47379", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47379", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47379" ], "product": "Uncharacterized protein MG133" } }, "id": "CNOCLNGIAL_611", "locus": "GCA000143945_00576" }, { "type": "cds", "sequence": "contig_1", "start": 323996, "stop": 324298, "strand": "+", "frame": 2, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_010874632.1", "SO:0001217", "UniParc:UPI000013932F", "UniRef:UniRef100_P75502", "UniRef:UniRef50_P47380", "UniRef:UniRef90_P75502" ], "nt": "ATGAGTTTTAAAAAGATTACCGAAATGATGCGCCAAGCTGAACGACAAAGCAAGCAAAAAGCGCTTGATTTCGAGCAAAAATTGTTTGAGTACAGCTATAAGAATGCTGCCATCAAGATTATCATTTTTGGTAATCTCACCATTAAGTCTATCACAATCGATCCCGCATTAATTGACCCAGAAGACAAGGTCACTTTAGAGGAGATGATTACAGAAGCTGTCAATGAGGCTGTGGGTGATGTTAAGGCCAAGTACGACCAGTTAATGGAAGAGGCTATGCCACAGATGCCTGGGCTTTTTTAA", "aa": "MSFKKITEMMRQAERQSKQKALDFEQKLFEYSYKNAAIKIIIFGNLTIKSITIDPALIDPEDKVTLEEMITEAVNEAVGDVKAKYDQLMEEAMPQMPGLF", "aa_hexdigest": "85a8ee1bfe713a32c945844c5f7bc40e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013932F", "ncbi_nrp_id": "WP_010874632.1", "uniref100_id": "UniRef100_P75502", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013932F", "RefSeq:WP_010874632.1", "UniRef:UniRef100_P75502" ] }, "ips": { "uniref100_id": "UniRef100_P75502", "uniref90_id": "UniRef90_P75502" }, "psc": { "uniref90_id": "UniRef90_P75502", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_P47380", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_P47380", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47380" ], "product": "Uncharacterized protein MG134" }, "genes": [ "ybaB" ], "id": "CNOCLNGIAL_612", "locus": "GCA000143945_00577" }, { "type": "cds", "sequence": "contig_1", "start": 324270, "stop": 324455, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCCTCAAGCTAGATCCAAAAAATGCTGATTTAATCTTTGGAGTTAGCTTTGGGTTGAGCTATTTCGCCATTGTATTGTTGACAGTTCTCGTCAACATTCCAACCTTTATACAGCTCACTAAAGATAAACAAATGGTTAAGCTAGCACTGCAGCTTAAAAAAGCCCAGGCATCTGTGGCATAG", "aa": "MFLKLDPKNADLIFGVSFGLSYFAIVLLTVLVNIPTFIQLTKDKQMVKLALQLKKAQASVA", "aa_hexdigest": "70b638538c507ceb45fa1c599637e4d5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6754.133800000002, "isoelectric_point": 9.778516197204588 }, "id": "CNOCLNGIAL_613", "locus": "GCA000143945_00578" }, { "type": "cds", "sequence": "contig_1", "start": 324483, "stop": 324581, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCGCTAGCATATCTCAGTTATGTGGCCTTGATGGTTAACATCTTTAAGGGACAAATTACCGGTAATACTACCGGCATTTTGGCTGAAGATTGA", "aa": "MFALAYLSYVALMVNIFKGQITGNTTGILAED", "aa_hexdigest": "8ae28675fb334f1ce0469fc7933e585d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3466.0291000000007, "isoelectric_point": 4.667413520812987 }, "id": "CNOCLNGIAL_614", "locus": "GCA000143945_00579" }, { "type": "cds", "sequence": "contig_1", "start": 324621, "stop": 325127, "strand": "-", "frame": 1, "gene": null, "product": "putative MG135-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47381", "UniRef:UniRef90_P75501" ], "nt": "ATGCAGTCGCTTAACTATCTTGTAGTCATCCTAACAGTAGCCGGGGTGCTAGTTATCTTGGGATTTACCCCATTAATTCGCAAATTAAAAATCCAGTTTTATTGTTTACAAGTTTTTGCAGCTATTCTCTTTCTCTATGTCTTTTTCGGGCGGCAAATTATCTATATTTTTCCCGATATTTACGGCACTGCAGCCAAGGCTAAGAATGCTGTTGCAAATGTTCCTTTAGATTCACTAAGGTTGAGCAGGATCTTTTTGTTAGATCTCTGCCCCTTCTTTGCCTTAATCGGCCCGATCTTTATCTTTTTACGCCAGAAAAAGGTGGCGGGTGTGTTGGCGATCTTTGGTTTTTACGGTGCTGCAATAACCTTATTTGGCGAGTTAATTTTTACTCCATTAAAACAAGAGGAGATTGTCAAATTTCTCTTTGTTGGTTTGGAAAACAACCAGGTTTACTTCATGATGCACTTCTTGAGTTTCTTGTTGAGTCTGGCTGTCTTTTTATGA", "aa": "MQSLNYLVVILTVAGVLVILGFTPLIRKLKIQFYCLQVFAAILFLYVFFGRQIIYIFPDIYGTAAKAKNAVANVPLDSLRLSRIFLLDLCPFFALIGPIFIFLRQKKVAGVLAIFGFYGAAITLFGELIFTPLKQEEIVKFLFVGLENNQVYFMMHFLSFLLSLAVFL", "aa_hexdigest": "1c2b5ac40e079a69c01ed1fba2dcbafb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 324318, "stop": 325127, "observations": { "mutation": [ 324485, 324623 ], "stop": [ 324485, 324623 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75501", "query_cov": 1.0, "subject_cov": 0.5894736842105263, "identity": 1.0, "score": 313.0, "evalue": 3.54e-106, "gene_start": 1, "gene_end": 168, "reference_start": 1, "reference_end": 168, "reference_sequence": "MQSLNYLVVILTVAGVLVILGFTPLIRKLKIQFYCLQVFAAILFLYVFFGRQIIYIFPDIYGTAAKAKNAVANVPLDSLRLSRIFLLDLCPFFALIGPIFIFLRQKKVAGVLAIFGFYGAAITLFGELIFTPLKQEEIVKFLFVGLENNQVYFMMHFLSFLLSLAVFLWDDGFSLISFFYIHVFALAYLSYVALMVNIFKGQITGNTTGILAEDWLSGEYKNVAVFLKLDPKNADLIFGVSFGLSYFAIVLLTVLVNIPTFIQLTKDKQMVKLALQLKKAQASVA" }, "paralog": false, "identity": 0.9925650557620818, "score": 514.0, "evalue": 1.2e-187, "description": "Internal stop codon at 324485, 324623. Nonsense mutation around 324485, 324623.", "psc": { "uniref90_id": "UniRef90_P75501", "product": "Uncharacterized protein MG135 homolog", "uniref50_id": "UniRef50_P47381", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75501", "UniRef:UniRef50_P47381", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47381", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47381" ], "product": "Uncharacterized protein MG135" } }, "id": "CNOCLNGIAL_615", "locus": "GCA000143945_00580" }, { "type": "cds", "sequence": "contig_1", "start": 325172, "stop": 325492, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCGATCGTTTAAATGATCAAGCACAAAATCGCTTGCAAAAACTCCTAAACCTCAAACAAACTGGCAACGATCCCTACCTCATTACTAAGATAGAAAACACCCACTCAGCGGCTAGTTTTCAAAAAGCATTTGCCAACCAATCTGACGCTGAGTTAAAGCAGTACCAAGTGATCTTAACCGGTCGGATTATTGCGCTCCGGCAGACCTTTATTATTATCCAAGACTTTAGTGGGAAAATGCAGTTGTACATTAATAAAAAACTGCCCCCGAACTGTTTGAATATTTCAATAATTACATTGATTTGGGTGATCAAATAG", "aa": "MSDRLNDQAQNRLQKLLNLKQTGNDPYLITKIENTHSAASFQKAFANQSDAELKQYQVILTGRIIALRQTFIIIQDFSGKMQLYINKKLPPNCLNISIITLIWVIK", "aa_hexdigest": "227be5b4e33ad6dcbe91f1bf247fe1ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12176.066499999988, "isoelectric_point": 9.721655082702636 }, "id": "CNOCLNGIAL_616", "locus": "GCA000143945_00581" }, { "type": "cds", "sequence": "contig_1", "start": 325603, "stop": 326049, "strand": "+", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6F12" ], "nt": "TTGACCGATCCAGAAGCGCGAGCACGAAAGCGTTACTTGGATTTGACCTACAACCGTGCGCAGGTCGAAGTCTTTTTGAAACGCACCCAGATTATTACAGCTATCCGTACCTTTTTAAATGAAGCGGGCTTTCTAGAAGTGGAAACACCCATTTTACAAGCCGTTTTAGGGGGTGCTAACGCCAAGCCCTTTAAAACACACTATAACGCCTTAAAGGGTGATTTTTACTTGCGGATTGCAAATGAAATAGCACTCAAAAAGCTCATTGTGGGCGGTTTGCCGAAAGTGTATGAAATGGGGCGGATGTTTCGTAACGAAGGGGTGGATACTACCCATAATCCGGAGTTTACGAGCATTGAAATGTATCAGGCTAACGCTGACTATGCGGTCATGATGGATTTAACCGAAAACCTCATTAAGTTTGTTTGCAAAACACTCAATCAGTGA", "aa": "MTDPEARARKRYLDLTYNRAQVEVFLKRTQIITAIRTFLNEAGFLEVETPILQAVLGGANAKPFKTHYNALKGDFYLRIANEIALKKLIVGGLPKVYEMGRMFRNEGVDTTHNPEFTSIEMYQANADYAVMMDLTENLIKFVCKTLNQ", "aa_hexdigest": "278eab2d7f0f5af08b4da585945887c5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000AFE6F12", "query_cov": 0.8918918918918919, "subject_cov": 0.8048780487804879, "identity": 0.598, "score": 160.0, "evalue": 9.83e-48, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI000AFE6F12" }, "pscc": { "uniref50_id": "UniRef50_UPI000AFE6F12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6F12" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "CNOCLNGIAL_617", "locus": "GCA000143945_00582" }, { "type": "cds", "sequence": "contig_1", "start": 326107, "stop": 326640, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGTAGATTTAATCTGCCAAGTTACTGGGGTTAATTTTGATGATGTTAAGGATGATCAAACAGCCATTGCGCTTGCAAAGCAGCACAAAGTAGAGTTAAAAAAGCACGAGCAGAACAAACAACACATTATTAACCGTTTCTTTGAACAGTTCTGTGAGCATACCTTAATCCAACCCACCTTTGTCACACACTATCCCAAGGCCGTTTCCCCGCTAGCTAAACAAGATCCCCACAACCCCGAGTTTACTGAGCGCTTTGAACTTTTTATTAATACAAAAGAGTTAGCGAACGCTTACAGTGAGTTGAATGATCCCCTAGAACAACGTGCGCGCTTTGAACAACAGTTGCAGGAAAAACGCATGGGTAATGATGAAGCGAGTGAATTAGATGAATCCTTTTTGGATGCTTTGAGTTTTGGTCTCCCGCCAACAGGCGGCCTTGGGATTGGGGTTGATCGGTTAGTGATGTTATTGTGCGAGTGCAGTTCCATCCGTGACGTTGTTTTCTTTCCCCAACTACGTGAATTGAAATAA", "aa": "MVDLICQVTGVNFDDVKDDQTAIALAKQHKVELKKHEQNKQHIINRFFEQFCEHTLIQPTFVTHYPKAVSPLAKQDPHNPEFTERFELFINTKELANAYSELNDPLEQRARFEQQLQEKRMGNDEASELDESFLDALSFGLPPTGGLGIGVDRLVMLLCECSSIRDVVFFPQLRELK", "aa_hexdigest": "478c23a9d37a17f0a5bc9f0b749d0bb3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031D0E1C7", "query_cov": 0.9830508474576272, "subject_cov": 0.8093023255813954, "identity": 0.563, "score": 191.0, "evalue": 1.05e-58, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A349PV97", "kegg_orthology_id": "K04567" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 127, "aa_cov": 0.6892655367231638, "hmm_cov": 0.968503937007874, "evalue": 1.2432495716376699e-37, "score": 125.01724243164062, "start": 52, "stop": 173 } ], "seq_stats": { "molecular_weight": 20361.885599999987, "isoelectric_point": 5.039197349548339 }, "id": "CNOCLNGIAL_618", "locus": "GCA000143945_00583" }, { "type": "cds", "sequence": "contig_1", "start": 326768, "stop": 327445, "strand": "+", "frame": 2, "gene": "glf", "product": "UDP-galactopyranose mutase", "db_xrefs": [ "COG:COG0562", "COG:M", "EC:5.4.99.9", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660", "KEGG:K01854", "SO:0001217", "UniRef:UniRef50_P75499", "UniRef:UniRef90_P75499" ], "nt": "TTGGATGAAACCACCGGGTTACTGTTCCACCAGTACGGTCCTCATATCTTTCATACTGATAATCAGAAAGTAATGGATTTTATCCAACCCTTTTTTGAACTAAACAATTACCAGCACCGTGTTGGCTTACAACTGGACAACAATTTGGATTTAACGCTTCCATTTGACTTTAGCCAAATGCGCAAGTTGTTGGATACAAAAACAGCTAGCAGTTTAATTAACTTCTTTCAACAGCACTTTCCAGCTGAAAAGCACTTAACACTGATGCAGTTACAAACAATTAACTTTGCTCCAGTCCAACAGCTGTACCAGTTTTTAAAAACAAAGGTGTACGGTCCTTACAGTGTGAAAATGTGGGGCATGCCATTAGAACAAATTGATCCTAGTGTATTGGGTCGAGTCAAAATTAGTTTAAGTGAAAACAGTAGCTATTTCCCTACAGCCACTATTCAAGGTCTACCTAAGGGTGGTTATACAAAGGCCTTTACAAAGATGGTGGATCATCCTCTGATTGATTTAAGGTTAAACTGTCCAGCTAACTTAATTAGCGTAAACAATAATCAACTCTTGTTTGCAAATCAACCAATTACCAAACCAGTGGTTTATTGTGGTTTAATTGATCAGCTGTTTGGCTATTGTTTTGGGCGATTGCAGTACCGCTCGCTCCACTTTGAATGA", "aa": "MDETTGLLFHQYGPHIFHTDNQKVMDFIQPFFELNNYQHRVGLQLDNNLDLTLPFDFSQMRKLLDTKTASSLINFFQQHFPAEKHLTLMQLQTINFAPVQQLYQFLKTKVYGPYSVKMWGMPLEQIDPSVLGRVKISLSENSSYFPTATIQGLPKGGYTKAFTKMVDHPLIDLRLNCPANLISVNNNQLLFANQPITKPVVYCGLIDQLFGYCFGRLQYRSLHFE", "aa_hexdigest": "0c698a9a94ff84fe7a9a69d7797f6445", "start_type": "TTG", "rbs_motif": null, "genes": [ "glf" ], "truncated": "both", "pseudogene": { "start": 326600, "stop": 327748, "observations": { "mutation": [ 326633, 327443 ], "stop": [ 326633, 327443 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75499", "query_cov": 1.0, "subject_cov": 0.5639097744360902, "identity": 0.991, "score": 463.0, "evalue": 1.2e-162, "gene_start": 1, "gene_end": 225, "reference_start": 57, "reference_end": 281, "reference_sequence": "MTLFSFPNYVNWNKFDFIVLGAGISGIVLSHVLAQHGKSVLLLEKRNQLGGNCYDKLDETTGLLFHQYGPHIFHTDNQKVMDFIQPFFELNNYQHRVGLQLDNNLDLTLPFDFSQMRKLLDTKTASSLINFFQQHFPAEKHLTLMQLQTINFAPVQQLYQFLKIKVYGPYSVKMWGMPLEQIDPSVLGRVKISLSENSSYFPTATIQGLPKGGYTKAFTKMVDHPLIDLRLNCPANLISVNNNQLLFANQPITKPVVYCGLIDQLFGYCFGRLQYRSLHFEWKRYAVKQHQAYPVMNWPLHPTITRQVEYKQLTQEGLESNQTIVSCETPGAFREGDPRFMEPYYPLNDVSNNALFARYLKLANAIPNIHLLGRLALYQYIDMDRAIAQSLAKAEQLLQ" }, "paralog": false, "identity": 0.9895287958115183, "score": 776.0, "evalue": 2.94e-288, "description": "Internal stop codon at 326633, 327443. Nonsense mutation around 326633, 327443.", "psc": { "uniref90_id": "UniRef90_P75499", "gene": "glf", "product": "UDP-galactopyranose mutase", "ec_ids": [ "5.4.99.9" ], "uniref50_id": "UniRef50_P75499", "cog_id": "COG0562", "cog_category": "M", "kegg_orthology_id": "K01854", "go_ids": [ "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75499", "EC:5.4.99.9", "UniRef:UniRef50_P75499", "COG:COG0562", "COG:M", "KEGG:K01854", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75499" ], "product": "UDP-galactopyranose mutase" } }, "id": "CNOCLNGIAL_619", "locus": "GCA000143945_00584" }, { "type": "cds", "sequence": "contig_1", "start": 327485, "stop": 327799, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTGGCCACTCCACCCAACAATAACGCGCCAAGTGGAATACAAACAGTTAACGCAAGAGGGTTTGGAGTCTAACCAAACGATTGTTTCGTGTGAAACTCCTGGTGCTTTTAGGGAAGGCGATCCCCGCTTTATGGAGCCGTACTACCCGCTAAATGATGTAAGTAATAATGCCTTGTTTGCGCGTTATTTAAAGTTAGCTAACGCTATTCCTAACATACATTTGTTAGGTCGGTTGGCACTGTACCAGTACATTGATATGGATAGAGCAATTGCTCAAAGCTTAGCTAAAGCCGAACAACTATTACAGTAA", "aa": "MNWPLHPTITRQVEYKQLTQEGLESNQTIVSCETPGAFREGDPRFMEPYYPLNDVSNNALFARYLKLANAIPNIHLLGRLALYQYIDMDRAIAQSLAKAEQLLQ", "aa_hexdigest": "85fcaea78d001ecb592da9f15d933603", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11938.490099999986, "isoelectric_point": 5.580077171325683 }, "id": "CNOCLNGIAL_620", "locus": "GCA000143945_00585" }, { "type": "cds", "sequence": "contig_1", "start": 327799, "stop": 329595, "strand": "+", "frame": 1, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "RefSeq:WP_014325436.1", "SO:0001217", "UniParc:UPI0001DD650F", "UniRef:UniRef100_A0AAV5N7J1", "UniRef:UniRef50_B1X3K4", "UniRef:UniRef90_P75498" ], "nt": "ATGGAACAACAAAAAATTAGGAACTTTTCAATTATTGCCCACATTGACCATGGTAAGTCAACGCTGTCAGATCGCTTAATTGAACGCAGCATTGGCTTTGAAAAACGCTTACTGCAAGCGCAAATGTTGGACACCATGGCGATTGAGCGTGAACGGGGTATTACTATTAAACTCAACGCCGTGCAGCTCAAAATGGCCCAAGGCAACCAGCAGTACTTGTTTCACCTAGTTGATACTCCGGGACACGTTGACTTTACTTACGAGGTATCACGCTCTTTAGCAGCTTGTGAGGGTGTTTTACTGTTAGTTGATGCTACCCAAGGGATTCAAGCACAAACGATTTCCAACACTTACTTAGCATTGGAAAACAACCTCGAAATTATTCCGGTAATTAACAAGGTGGACATGGAGAGTGCTGATGTCGAGAAAACCAAACAAGCCTTTCACCAACTGCTAGGGGTGGATCCCAACACCATCCCCTTAGTGTCCGCTAAAACCGGGCTTGGTATTGACCGGTTAATCACTACCATTATTGAAAAAGTACCCCCTCCAAAGGGCGATGAAAGTAAACCACTCAAAGCCTTACTGTTTGACAGCTATTACGATCCTTATAAGGGGGTAGTGTGCTTTATTCGAATCTTTGAGGGGAGTTTAAAACTCAATGATAAGATCCGCTTTGCTAGAAGTAACTCGGTTTACCAAATTGTGGAATTGGGAATTAAAAACCCCTTTTTTGAAAAACAAGACGTGTTAAAAGCCGGGGAGATTGGTTGGTTTTCCGCTGGGATTAAAAAACTAAGGGATGTAACGGTTGGTGACACGATTGTTCACGCTGAGGACACGACCACTCCACCCTTACCGGGTTACAAAAAGGTGTTACCGATGATTTACTGTGGTCTGTACCCGATTGACAACAATGATTACCAAAACCTCAAAATGGCCATGGAAAAGATTATTTTGAGCGATGCGGCCTTGGAGTATGAATATGAAACATCACAAGCATTGGGCTTTGGGGTGCGTTGTGGTTTTTTGGGGTTGTTGCACATGGATGTAATTAAGGAGCGGTTGGAGCGCGAGTACAACCTTAAGTTAATTTCCGCTCCTCCTTCAGTGCGGTATAAGGTGTTGTTAACCAACGGGGAGGAATTGGAACTCGATAACCCTTCACTCTTACCAGAGCGCTCGCGAATTAAATCAATTAGCGAACCGTTTGTACGCGTTTACATTGATCTACCCGATCATTATTTAGGTACAGTAATTGATCTGTGTCAAAACTTCCGTGGGCAATACGAAAAGTTAGAGGAAATTGACATTGACCGCAAACGCTTGGTCTACTTAATGCCCTTGGGTGAAATTATCTACAGTTTCTTTGATAAGTTGAAATCAATCACTAAGGGTTATGCTTCGCTCAATTACGAGTTTGACCAGTACCAGGTGTCCCAATTGGCTAAGGTGGAAATTATGTTAAACAAACAGAAGGTGGACGCTTTATCTTTTATTGCCCACCATGACTTTGCCTTTCAACGGGCCAAGAAGTTCTGTGTCAAATTAAAGGAACTAATTCCCAAGCACCTGTTTGAAATTCCAATTCAAGCCACAATTGGAAGTAAGGTAATTGCTAGAGAAACGATTAAAGCAGTGCGTAAAGATGTAACAGCAAAGCTGTATGGCGGGGATGTCACGCGGAAAAAGAAGCTGTTGGAAAAACAAAAGGAAGGGAAGAAAAGACTGAAAGCAATTGGTAGTGTGGAGCTCCCCCAAGAGCTGTTTAGTCATTTGCTCAAAGATGAAGATTAA", "aa": "MEQQKIRNFSIIAHIDHGKSTLSDRLIERSIGFEKRLLQAQMLDTMAIERERGITIKLNAVQLKMAQGNQQYLFHLVDTPGHVDFTYEVSRSLAACEGVLLLVDATQGIQAQTISNTYLALENNLEIIPVINKVDMESADVEKTKQAFHQLLGVDPNTIPLVSAKTGLGIDRLITTIIEKVPPPKGDESKPLKALLFDSYYDPYKGVVCFIRIFEGSLKLNDKIRFARSNSVYQIVELGIKNPFFEKQDVLKAGEIGWFSAGIKKLRDVTVGDTIVHAEDTTTPPLPGYKKVLPMIYCGLYPIDNNDYQNLKMAMEKIILSDAALEYEYETSQALGFGVRCGFLGLLHMDVIKERLEREYNLKLISAPPSVRYKVLLTNGEELELDNPSLLPERSRIKSISEPFVRVYIDLPDHYLGTVIDLCQNFRGQYEKLEEIDIDRKRLVYLMPLGEIIYSFFDKLKSITKGYASLNYEFDQYQVSQLAKVEIMLNKQKVDALSFIAHHDFAFQRAKKFCVKLKELIPKHLFEIPIQATIGSKVIARETIKAVRKDVTAKLYGGDVTRKKKLLEKQKEGKKRLKAIGSVELPQELFSHLLKDED", "aa_hexdigest": "48972e499ad8842e3bb6347000878b1d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD650F", "ncbi_nrp_id": "WP_014325436.1", "uniref100_id": "UniRef100_A0AAV5N7J1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD650F", "RefSeq:WP_014325436.1", "UniRef:UniRef100_A0AAV5N7J1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N7J1", "uniref90_id": "UniRef90_P75498" }, "psc": { "uniref90_id": "UniRef90_P75498", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_B1X3K4", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "genes": [ "lepA" ], "id": "CNOCLNGIAL_621", "locus": "GCA000143945_00586" }, { "type": "cds", "sequence": "contig_1", "start": 329674, "stop": 331377, "strand": "+", "frame": 1, "gene": "rnj", "product": "Ribonuclease J", "db_xrefs": [ "COG:COG0595", "COG:J", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0004534", "GO:0005737", "GO:0006364", "GO:0008270", "SO:0001217", "UniRef:UniRef50_P75497", "UniRef:UniRef90_P75497" ], "nt": "ATGATTAAGGATTTTGATCCTAGTGAGTTTGTCGGTAAAACTCCTACCAAGATTTTTGCCTTTGGTGGTATCCAAGAAGTTGGAAAAAACATGTATGGAATCGAGTACGATGATGAGATTATCATCATCGACTGCGGTATTAAGTTTGCTAGTGATGATCTACTAGGGATTGATGGTATTATCCCGAGCTTCGAATACCTCATTGAAAACCAAGCTAAGGTTAAAGCACTGTTTATCACCCACGGCCATGAAGACCACATTGGTGGGGTGCCTTACTTGTTAAAACAAGTCGATGTACCGGTGATTTACGCACCCCGGATTGCCGCTTCTTTGATCTTAAAGAAGGTCAACGAACACAAGGATGCTAAGCTCAATAAAGTGGTCGTATATGATGACTTTAGCAACTTTGAAACCAAGCACTTCAAGATTGACTTTTACCGCGTCAACCACTCGATTCCTGACGCCTTTGGTGTCTGTGTTCAAACTCCCAACGGTAACATTGTTGAGAGTGGTGACTTTCGCTTTGACTTTGCCGCTGGCGGGGAAATGCTCGATGTGCATAAGGTCGTGAAGATTGCCGAGCGCAATGTCCATGTCTTTATGTGTGAGACTACTAACGCCGAAATCCCGGGGTTCTCCCAGAGCGAAAAGTTAATTTACCGCAACATCAACAAGATTATTAAAGAAGCACGTGGTCGGGTCATTCTCACTACCTTTGCTTCCAACATTACCCGGATTAACGAAATTATTGAGATTGCTGTTAACAATAAACGGAAGGTCTGCTTGTTGGGTAAGTCGATGGATGTGAACGTCAACATCTCGCGTAAGATTGGCTTAATGGACATTGACAGCAATGACATTGTGGAAGTCCGTGACATTAAGAATTACCCCGACCGCAGTATCTTAATCTTGTGTACGGGTTCCCAAGGTGAAGACTCAGCAGCCTTAAACACAATGGCACGGGGTAAACATAACTGGGTTAGCTTAAAGTCCACTGACACCATTATTATGTCCTCCAACCCGATTCCGGGGAACTATGCAGCGGTGGAAAATCTCTTGAATGAGCTTTCGAAGTACGGGGTGACCATCTTTGAAAACTCACCGAACATGAAGCTACACGCCTCTGGTCACGCTACCCAGCAAGAGTTGCAGTTAATGTTAAACCTCGTGTTTCCAAGGTACTTAATTCCGATTCACGGGGAGTACAAGATGATGCGCACCATCAAAAACATAGCGCAAGAATGTGGCATTAACGGTGATGATGTTGGTTTGCTAGCTAACGGTCAGGTGATGTACCTAATTGATGGCAAGCTGTATTACTCAGGGGAAGTGATTAATGCTGACCCGATTTACATTGAAAGCCGCAATTCTTCTCCAGACTTAGCGCGGGTAATTAAACAACGCCAAATCTTGAGTCGGGAGGGAATGTTTGCTGTGATTGTAGTCTTTGACAAAAACAATAACATTTTAGGGATGCCTACTCTGATTACTCGGGGTTGTTTCTTTGCTCTAGATTCCAGTCCGTTAATGACTAAGATTACCCACTCAATTAAACGCGGTTTGGAAAACGTTATTCAAAACAAGCGTTTTAATACCCGTGAACAGATGATCAAGGAATTGAAACGAGTATGTAAGGAAACGGTGTCTTACTTCATTTGGAAGAACAAGAGTCGTAACCCGTTGATTTCAACCGTATTGTCTTGA", "aa": "MIKDFDPSEFVGKTPTKIFAFGGIQEVGKNMYGIEYDDEIIIIDCGIKFASDDLLGIDGIIPSFEYLIENQAKVKALFITHGHEDHIGGVPYLLKQVDVPVIYAPRIAASLILKKVNEHKDAKLNKVVVYDDFSNFETKHFKIDFYRVNHSIPDAFGVCVQTPNGNIVESGDFRFDFAAGGEMLDVHKVVKIAERNVHVFMCETTNAEIPGFSQSEKLIYRNINKIIKEARGRVILTTFASNITRINEIIEIAVNNKRKVCLLGKSMDVNVNISRKIGLMDIDSNDIVEVRDIKNYPDRSILILCTGSQGEDSAALNTMARGKHNWVSLKSTDTIIMSSNPIPGNYAAVENLLNELSKYGVTIFENSPNMKLHASGHATQQELQLMLNLVFPRYLIPIHGEYKMMRTIKNIAQECGINGDDVGLLANGQVMYLIDGKLYYSGEVINADPIYIESRNSSPDLARVIKQRQILSREGMFAVIVVFDKNNNILGMPTLITRGCFFALDSSPLMTKITHSIKRGLENVIQNKRFNTREQMIKELKRVCKETVSYFIWKNKSRNPLISTVLS", "aa_hexdigest": "d21bd145e41819c6527a6f3dc701c2a8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75497", "query_cov": 1.0, "subject_cov": 0.9964850615114236, "identity": 1.0, "score": 1127.0, "evalue": 0.0, "valid": true, "gene": "rnj", "product": "Ribonuclease J", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75497", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0004534", "GO:0003723", "GO:0004521", "GO:0008270", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_P75497", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75497" ], "product": "Ribonuclease J" }, "genes": [ "rnj" ], "id": "CNOCLNGIAL_622", "locus": "GCA000143945_00587" }, { "type": "cds", "sequence": "contig_1", "start": 331594, "stop": 332223, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75496", "UniRef:UniRef90_P75496" ], "nt": "ATGAAATTTAAGTATGGAACGGTTGTTTTGGGCAGTTTTTTAGGGCTTTCTGTGGTGTTAGCTGCCTGTGGTGCTCGTGGTAAATTTGACCAATTTGATGATGGCAAAATTAAGCTAGCATCATCCTTAACTTCCAAAGCAGCTGCCAATGCTTTACAAACAATAATAGAGAAGTACAACATTGTCAAAAGCGGCAAGGATTACCCCATTGAAATTACCCAAATTGCTGGTGGTTATGACGGCGGGCGGACTGATCTCCAAACCCGAGTTAGTGTTAAGGATAAAACGAACTTTTATAACCTTATTTTGAACTATCCAGATTTAGTTTCTGTGTTAGCGCGCAGTGGAATGGAACTGCTTTTTGACAAAGTTAATGTTGATAAATTAGAGCCGAACTTCTTAAAGTTTAATGAACAGATTAGTGGAGTCGCTAAAAGTGGTAACTATGGTATTCCCGTGTCCCTTTCAACTGACATTTTGGTCTTAAATGGTCCGGTGTTGCACTATATTTTGAACAGTGCGAAGAAAGAAGAGGCTAATTCTCAAGTTAAAAGTCAAATAAAAACATCACAAATTCAAGCTAAAGGTTCATTAACAATTGATACAGATGAAAATACCAAATCACTTTGA", "aa": "MKFKYGTVVLGSFLGLSVVLAACGARGKFDQFDDGKIKLASSLTSKAAANALQTIIEKYNIVKSGKDYPIEITQIAGGYDGGRTDLQTRVSVKDKTNFYNLILNYPDLVSVLARSGMELLFDKVNVDKLEPNFLKFNEQISGVAKSGNYGIPVSLSTDILVLNGPVLHYILNSAKKEEANSQVKSQIKTSQIQAKGSLTIDTDENTKSL", "aa_hexdigest": "c1111ab55381545f965bdb3047286aef", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 331594, "stop": 332526, "observations": { "mutation": [ 332221, 332392 ], "stop": [ 332221, 332392 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75496", "query_cov": 1.0, "subject_cov": 0.5543766578249337, "identity": 1.0, "score": 401.0, "evalue": 9.03e-139, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MKFKYGTVVLGSFLGLSVVLAACGARGKFDQFDDGKIKLASSLTSKAAANALQTIIEKYNIVKSGKDYPIEITQIAGGYDGGRTDLQTRVSVKDKTNFYNLILNYPDLVSVLARSGMELLFDKVNVDKLEPNFLKFNEQISGVAKSGNYGIPVSLSTDILVLNGPVLHYILNSAKKEEANSQVKSQIKTSQIQAKGSLTIDTDENTKSLWEKIQNSAKANGETNQKGRKAAKSNKTALVQLKNGADTTTNEENKDTKTSDDKVKQTWGDYVEKDDGLKNYTFRASVFENWHDFLDFSTRVAKSFTEKVSNITNKKGTDIQGVLGVDSSPNVLFASVFAAGGGNYENFFYKLKDGRADFSNFKNKGSSYQNLQSVFKD" }, "paralog": false, "identity": 0.9935483870967742, "score": 599.0, "evalue": 3.94e-219, "description": "Internal stop codon at 332221, 332392. Nonsense mutation around 332221, 332392.", "psc": { "uniref90_id": "UniRef90_P75496", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75496", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75496", "UniRef:UniRef50_P75496", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75496", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75496" ], "product": "Uncharacterized lipoprotein MPN_281" } }, "id": "CNOCLNGIAL_623", "locus": "GCA000143945_00588" }, { "type": "cds", "sequence": "contig_1", "start": 332449, "stop": 332727, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGAAAACTGGCATGATTTCCTCGATTTTTCGACAAGGGTAGCTAAATCTTTTACTGAAAAGGTAAGCAACATTACCAATAAAAAGGGCACTGATATTCAGGGAGTGCTTGGTGTCGACAGTTCTCCTAATGTTTTATTTGCCTCTGTATTTGCTGCAGGTGGTGGTAATTATGAAAACTTCTTTTATAAGGTGAAAGATGGTCGGGCTGATTTTAGTAACTTTAAGAACAAGGGTAGTTCTTACCAAAACCTCCAAAGCGTTTTTAAAGACTAA", "aa": "MFENWHDFLDFSTRVAKSFTEKVSNITNKKGTDIQGVLGVDSSPNVLFASVFAAGGGNYENFFYKVKDGRADFSNFKNKGSSYQNLQSVFKD", "aa_hexdigest": "43fb4cdc938be627dcbc21d2ae921c39", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10307.276099999994, "isoelectric_point": 7.965665245056153 }, "id": "CNOCLNGIAL_624", "locus": "GCA000143945_00589" }, { "type": "cds", "sequence": "contig_1", "start": 332872, "stop": 333582, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FE0B92", "UniRef:UniRef100_A0AB38W7P6", "UniRef:UniRef50_A0AB38W7P6", "UniRef:UniRef90_A0AB38W7P6" ], "nt": "TTGAAGTTTAGTGACGACAACACCTTCGTTGAATATCCTGATTTTACTCAGGAAATTAAGTCGCCAGACCAACAAAATGGTCAAAATACTGATGACAATAACAGTAAAAAAGAAGAAAACCTTTTAGGTACCTTTGAAGTTAAAGATTACTCGAAATCTAATAAAGGCGGGAAAGGAAAAAGTAAGGGTAAAGGCGGAAGCAGCAGTAATATGAAGACTATTTACATTTACAAAGGCTATATTCCTGAAGATAAAAAGAAAGGTGATAATGCCATTTTAGTTACTGAGAAAAAAATTATTGATGCTTTAGAAAAAGCTGCTAATTCTAATGGTGAAAGTACACAATCTAATCCAAAAACCATAGCACTTCAAGCAGCTAAAAAAGAAACTAAAAAGAGCAATAAGAAAGAATCAAAAACGATTGGATATACAACTACTGGTTATGTCAATGCTGATGGTAAACATATTTTTGATACAGACAGAATTAACAATGAGAGGTTTGATCGTAAAATTATTATTGGTGCTACTCTAGAAACTTTAGACCAATCTAGTACACTCCAAAGTGGTGAAGCAATTGTCTTACCAGCACCTGGTAAATATAAGAGTAGTGATCAAAAAACGGTTATGACAGCGCAAGGCCCCAAGATAATCGGGATCCATGCTAATGCTAAAGAGAACGAAGAAACACAAAAATTTGTTAATTGGTTTTAA", "aa": "MKFSDDNTFVEYPDFTQEIKSPDQQNGQNTDDNNSKKEENLLGTFEVKDYSKSNKGGKGKSKGKGGSSSNMKTIYIYKGYIPEDKKKGDNAILVTEKKIIDALEKAANSNGESTQSNPKTIALQAAKKETKKSNKKESKTIGYTTTGYVNADGKHIFDTDRINNERFDRKIIIGATLETLDQSSTLQSGEAIVLPAPGKYKSSDQKTVMTAQGPKIIGIHANAKENEETQKFVNWF", "aa_hexdigest": "981289ee2675336c22943859cd7dbb5b", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002FE0B92", "uniref100_id": "UniRef100_A0AB38W7P6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FE0B92", "UniRef:UniRef100_A0AB38W7P6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7P6", "uniref90_id": "UniRef90_A0AB38W7P6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W7P6", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AB38W7P6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7P6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7P6" ], "product": "Lipoprotein" }, "genes": [], "id": "CNOCLNGIAL_625", "locus": "GCA000143945_00590" }, { "type": "cds", "sequence": "contig_1", "start": 333678, "stop": 333953, "strand": "+", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594", "UniRef:UniRef90_A0AAN4XG79" ], "nt": "TTGCCCTTAAAAGAAAACTTTGAAAAGGTTCAAATTAACGATAAAGGTGAGAAAAATGAAAATGACACAAAGGGCAATACTTATACCGCTAAAGCTTTAGAACTCTTTAAAAAAGTTTCTCAAAATGAAATCGTGAGTTACAGTGACCCAAGTGACTTTAGAAGTGGTAAGTTTCGTGATGCGATTGGCTCTAACTTTAACGCCATTATTAATTCCAAAGGAGACTTCAATAAATTTATTAGTAGTTTTACCGCAAGTTTAGGCTCAGACTTTTAG", "aa": "MPLKENFEKVQINDKGEKNENDTKGNTYTAKALELFKKVSQNEIVSYSDPSDFRSGKFRDAIGSNFNAIINSKGDFNKFISSFTASLGSDF", "aa_hexdigest": "f0d0eb731e90b937640d853f7361250a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 333573, "stop": 333953, "observations": { "mutation": [ 333597 ], "stop": [ 333597 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN4XG79", "query_cov": 1.0, "subject_cov": 0.7222222222222222, "identity": 0.9890000000000001, "score": 175.0, "evalue": 5.05e-55, "gene_start": 1, "gene_end": 91, "reference_start": 36, "reference_end": 126, "reference_sequence": "MVLNTPETWESKRNKKNSNEKQTAAQYFAQEASYILPLKENFEKVQINDKGEKNENDTKGNTYTAKALELFKKVSQNEIVSYSDPSDFRSGKFRDAIGSNFNAIINSKGDFNKFISSFTASLGSDF" }, "paralog": false, "identity": 0.9841269841269841, "score": 246.0, "evalue": 1.25e-85, "description": "Internal stop codon at 333597. Nonsense mutation around 333597.", "psc": { "uniref90_id": "UniRef90_A0AAN4XG79", "product": "Lipoprotein", "uniref50_id": "UniRef50_P75594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN4XG79", "UniRef:UniRef50_P75594" ] }, "pscc": { "uniref50_id": "UniRef50_P75594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594" ], "product": "Uncharacterized lipoprotein MPN_098" } }, "id": "CNOCLNGIAL_626", "locus": "GCA000143945_00591" }, { "type": "cds", "sequence": "contig_1", "start": 334478, "stop": 334795, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6513", "UniRef:UniRef100_A0A0H3DL71", "UniRef:UniRef50_A0A0H3DL71", "UniRef:UniRef90_A0A0H3DL71" ], "nt": "TTGAAGTTATTTTGTCACATTTACTTTTTGGGCAATTTAGATCATGAATACCGAAAAAGTATAAGATTCATTAAGTTAACGAGGTATCACCTCTTTTTAAAACAAGCCCAATCGCAACTCGTTTCTTTGTTTAACCAAAACTTTTTGCAAGTTTTGTTGGCATTTCTAAATAAATCTTTCAATTTATTTAGTGATGACACAAAAATTAATATGTTGAACTTTTTAAATAAATCAACAATGGTATTGATCCCCGTTGGCAGCAGTGCTCCAAGTTCTAGTTCCTTTTTAAATCACCTCAAGAAAGAAGGTATTGCTTAA", "aa": "MKLFCHIYFLGNLDHEYRKSIRFIKLTRYHLFLKQAQSQLVSLFNQNFLQVLLAFLNKSFNLFSDDTKINMLNFLNKSTMVLIPVGSSAPSSSSFLNHLKKEGIA", "aa_hexdigest": "a72389eee8571bf51ad05d84a99f5ac5", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6513", "uniref100_id": "UniRef100_A0A0H3DL71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6513", "UniRef:UniRef100_A0A0H3DL71" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DL71", "uniref90_id": "UniRef90_A0A0H3DL71" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DL71", "uniref50_id": "UniRef50_A0A0H3DL71" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DL71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DL71" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12175.143799999983, "isoelectric_point": 9.868578529357908 }, "id": "CNOCLNGIAL_627", "locus": "GCA000143945_00592" }, { "type": "cds", "sequence": "contig_1", "start": 334795, "stop": 334905, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAGAAAATTTCTAAAAAGGAATACAATGCCTTAATCCGTAAAACTGGCGAAAAGCACTTTGACGGCGAAAAGGAAGAGTACGGTGATGGCACCGTTGGACTTTGA", "aa": "MKEKISKKEYNALIRKTGEKHFDGEKEEYGDGTVGL", "aa_hexdigest": "d16543a5863734ff111d693cff88e39d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4129.604900000002, "isoelectric_point": 6.5516260147094725 }, "id": "CNOCLNGIAL_628", "locus": "GCA000143945_00593" }, { "type": "cds", "sequence": "contig_1", "start": 334942, "stop": 335142, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAAAGTTAAATACGTAACGCAAGAAGAGTTTGGCGAATTCAAAGATGCCACCAACCAGCGGCTCACAAAGATTGAGAACGCTTTGGTCGCTCAGGGCGAACAAATTAGAGCTCAGGGTGAGCAATTAAGTCAACTAATTAAAGTTGTGCTTCTTCAGGGCAAACAAATAAAATCTCAAGGTGAGCAAATAAAATCTTAG", "aa": "MKVKYVTQEEFGEFKDATNQRLTKIENALVAQGEQIRAQGEQLSQLIKVVLLQGKQIKSQGEQIKS", "aa_hexdigest": "11840b37ae2af7d8cb4eb7115dfd54e0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 64, "aa_cov": 0.9696969696969697, "hmm_cov": 1.0, "evalue": 5.843458654120624e-15, "score": 51.165557861328125, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 7475.494, "isoelectric_point": 9.099599647521973 }, "id": "CNOCLNGIAL_629", "locus": "GCA000143945_00594" }, { "type": "cds", "sequence": "contig_1", "start": 335580, "stop": 335828, "strand": "+", "frame": 3, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "RefSeq:WP_015344899.1", "SO:0001217", "UniParc:UPI0002B8464C", "UniRef:UniRef100_A0AAP8JFL4", "UniRef:UniRef50_A0AAP8E4D4", "UniRef:UniRef90_A0AAP8JFL4" ], "nt": "ATGAAAAAAGTTAAAGTTCTAGAGAAGCCATCTGTTGAAAGAATGATAGAAGTTTTAAGCGACTCTGAAATATTGAAGTTTGATTTTTCTTGCTATTATTTACCTGATGGTACTAATGAGACGCTCATAAGAATCTTTTTTCAAGAAAAAGTTTCAAGTTCTGTAGTAACAAAAGATAAAAATTCTTTAAGACTTGATGAATACTTAAAAGACTTTATTAGCTTCTTAAAAACACAAAGAAAAAACTAA", "aa": "MKKVKVLEKPSVERMIEVLSDSEILKFDFSCYYLPDGTNETLIRIFFQEKVSSSVVTKDKNSLRLDEYLKDFISFLKTQRKN", "aa_hexdigest": "6dad6782f1b05f336bf0127f2ff65eb9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B8464C", "ncbi_nrp_id": "WP_015344899.1", "uniref100_id": "UniRef100_A0AAP8JFL4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B8464C", "RefSeq:WP_015344899.1", "UniRef:UniRef100_A0AAP8JFL4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8JFL4", "uniref90_id": "UniRef90_A0AAP8JFL4" }, "psc": { "uniref90_id": "UniRef90_A0AAP8JFL4", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D4" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4" ], "product": "Family K-like protein" }, "genes": [], "id": "CNOCLNGIAL_630", "locus": "GCA000143945_00595" }, { "type": "cds", "sequence": "contig_1", "start": 336086, "stop": 336868, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTGAAATATGGAACCATTATTTTCAGTGGTCTTTTGGGAGTTTCCGCCATTTTAGCTGCCTGTGGTGCACGCGGCAAATTTGACCAAATTGATGATGGCAAAATTGTTTTAGCTTCTTCTCTTACATCTAAGGGTGCAGCTAATGCCTTGCAAACAATTGTTAAAAAGTACAACGAAGTTAAAAATATTGATGACTATCCCATTGAAATTATCCAAATTGCCGGTGGTTATGATGGCGGTCGTGGTAACTTACAAACAAAACTAAGTGTTAAAGACAAAAATAGTTTCTATAACTTAATTTTGAACTACCCAGACGTAGTTTCTGTTTTAGGTCGAGTAGGCATGGAATTGCCCTTCGATAAGGTTAGAACAGATAAATTATCACCCCGTTTTCTTGATTTCAACAAGCGAATTAGTGCCATATCTAAGCAAGGTATTTACGGCATTCCAGTCTCTTTGTCTACTGAAGTTTTAGTTTTAAATGGTCCGGTATTGCACTATATTTTGAGCAGTGCAAAGGGTAGTAGTGACAAAACGCAAGTAAGTCAAACATCATCAGGATTAAATCAACAAAAAACTCTTCAAAAACCCTTAAAGATTGATACAAGCGATTCAAGCACTTCTTCTCTTTGGACTCAAATTGAGAACGCCGCTAAAAATAACGGTAAAAAAGCAAATAATAGCAAAAGCAATAGAAGATCCACAGATCAAAGTACACAAACACATAACGATCAAGGCGATGCTTCTGAAAGTGATAAAAAGATTAAAGAATCTTGA", "aa": "MKLKYGTIIFSGLLGVSAILAACGARGKFDQIDDGKIVLASSLTSKGAANALQTIVKKYNEVKNIDDYPIEIIQIAGGYDGGRGNLQTKLSVKDKNSFYNLILNYPDVVSVLGRVGMELPFDKVRTDKLSPRFLDFNKRISAISKQGIYGIPVSLSTEVLVLNGPVLHYILSSAKGSSDKTQVSQTSSGLNQQKTLQKPLKIDTSDSSTSSLWTQIENAAKNNGKKANNSKSNRRSTDQSTQTHNDQGDASESDKKIKES", "aa_hexdigest": "76fb099f4fa871e16b03a4ae902ccf32", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28164.512700000025, "isoelectric_point": 9.487441444396975 }, "id": "CNOCLNGIAL_631", "locus": "GCA000143945_00596" }, { "type": "cds", "sequence": "contig_1", "start": 336884, "stop": 338086, "strand": "+", "frame": 2, "gene": null, "product": "P80 family lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B", "UniRef:UniRef90_UPI001330F80B" ], "nt": "GTGGATGGTGGCTTAAAAGGCTTTACTTTTAAAGCTAGCATCTTTGATAATTGGCACGATTTACTCGATTTTTCTACTAGAGCGGCTAAATCTTTTAAAAAGATAAAAGATAACAATACTAAAAAAGGAACTGATATCCAAGGCATTCTTGGTGTAGATAGTTCAGCCAATTCCTTATTTACTTCTGTTTTTGCAGCTGGTAATGGTGACTATGACAACTTCTTTTACAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAGAGGTAGTTCCTTCCAAAACTTACAAAGTGTTTTTAATGATTACAAGGGTTTAATTGATCAAAACGGTCTCTTTGTTAATAAGGGCGGTTCTTACTCTTCTAACTTTCAAAAGTTTCACCAGTTAGCTTACAGTATCTCCTCTACCTCTGGTTTCTATTATTCATTTGCTGGTAATAGTGCTAAGCGCTTAAAGTTTGGTGATAATTCTTTCATTGAGTATCCACAATATACTGTTCCAATTAAAGCTCCTTCAAAGAATGGTGATGGAAATAGCACAAACAGCAATAGCGATCTTTTAGGCACTTTCACTTTAAGTAGTGGTAAAAAGTCAACAGATAAAAGTAAAAGTGATAGTCAACAAAATCAAGGCAAGAAAGTAGAAGGAACACCTAACCAGGGTAAAAAGGCAGAAGGTGCCCAGAACCAAGGCAAAAAAGAAAATAACAGTACAACTATTGAAATTTACAAGAACAAAATTCCTGATGGTAAAAATGCTGGGAAAGATGCCATCTTAATAAAAGATAACAATCTCATTAAACAGCTAGAAGATGCTGCTAAGAAAAACGGTGCAGAAAGTAATCAAAAACAAGGTGGAGAGAGTAATGGTCAGAAAGAACAAATTATTGGTTATACCACCACTGGTAACGTTCGTGAAGATGGTAATCACATTTTTAGAGTAGACAAAATTAATGACGAACAATACGATCGCAAAATTATTGTTGGCGTTACTGTGGAAACTTTAGAACAGTCTAGCACTTTGCAAAGCGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAATATAAAAGCACTGATAAAAAGAAGGTAACAATTACCCAAGGTCCGAATATTATTGGTATCCATGCTAACGAAAAAGAAAATGCAGAAACCCAAAAGTTTGTTGACTGGTTTTTAAATACAGAAGTAGATTGA", "aa": "MDGGLKGFTFKASIFDNWHDLLDFSTRAAKSFKKIKDNNTKKGTDIQGILGVDSSANSLFTSVFAAGNGDYDNFFYKVANGRADFSNFKNRGSSFQNLQSVFNDYKGLIDQNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFYYSFAGNSAKRLKFGDNSFIEYPQYTVPIKAPSKNGDGNSTNSNSDLLGTFTLSSGKKSTDKSKSDSQQNQGKKVEGTPNQGKKAEGAQNQGKKENNSTTIEIYKNKIPDGKNAGKDAILIKDNNLIKQLEDAAKKNGAESNQKQGGESNGQKEQIIGYTTTGNVREDGNHIFRVDKINDEQYDRKIIVGVTVETLEQSSTLQSEEAIVLAAPGKYKSTDKKKVTITQGPNIIGIHANEKENAETQKFVDWFLNTEVD", "aa_hexdigest": "62672c6de014e2348fc6458f22184733", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001330F80B", "query_cov": 1.0, "subject_cov": 0.8528784648187633, "identity": 0.998, "score": 734.0, "evalue": 1.68e-265, "valid": true, "product": "P80 family lipoprotein", "uniref50_id": "UniRef50_UPI001330F80B" }, "pscc": { "uniref50_id": "UniRef50_UPI001330F80B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B" ], "product": "P80 family lipoprotein" }, "genes": [], "id": "CNOCLNGIAL_632", "locus": "GCA000143945_00597" }, { "type": "cds", "sequence": "contig_1", "start": 338560, "stop": 338739, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGA", "aa": "MEIKTYKIKDICDIKRGRVISKLDIKKDPGVFPVYSAATNNDGEFGRINSYDFDGEYVT", "aa_hexdigest": "6973df725003fab248d0403e95c16b3e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 61, "aa_cov": 0.9661016949152542, "hmm_cov": 0.9836065573770492, "evalue": 3.761052923832423e-11, "score": 38.95970153808594, "start": 3, "stop": 59 } ], "seq_stats": { "molecular_weight": 6735.564300000002, "isoelectric_point": 6.130108070373535 }, "id": "CNOCLNGIAL_633", "locus": "GCA000143945_00598" }, { "type": "cds", "sequence": "contig_1", "start": 338720, "stop": 338815, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGA", "aa": "MVNMLLEQLTVMEGLFFIVMVNLASQIFVVF", "aa_hexdigest": "4165f2c157215872847f65036bab0f87", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3577.3857, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_634", "locus": "GCA000143945_00599" }, { "type": "cds", "sequence": "contig_1", "start": 338929, "stop": 339450, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q50287", "UniRef:UniRef90_UPI0027E16080" ], "nt": "ATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGAGAGCGCAGGAAGCAGTATGACTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAATAAGGGATATTTGTGAAATAAATCGTGGTCGAGAAATAAATGAAAAATACCTTCGAGAAAATCCAGGTGAATTTCCAGTTTATTCCTCAGCAACAACAAATGGTGGTTTAATTGGAAAAATTAATGATTACGATTTTCATGGTGAATATGTTACTTGA", "aa": "MAEIPIDFPPLKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELRERRKQYDFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVT", "aa_hexdigest": "f86b3f8873c9873f5abe5b3b4dcbf961", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 338887, "stop": 339753, "observations": { "mutation": [ 339448 ], "stop": [ 339448 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0027E16080", "query_cov": 1.0, "subject_cov": 0.5616883116883117, "identity": 0.9079999999999999, "score": 201.0, "evalue": 1.71e-61, "gene_start": 1, "gene_end": 173, "reference_start": 15, "reference_end": 175, "reference_sequence": "NRVFKNRPKLTHKTMAEIPIDFPPLKIQEKIATILDTFTELSTELSTELSTELSAELSAELSAELSAELSAELSAELSAELSAELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVTWTTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNWVQNKKLESLKSL" }, "paralog": false, "identity": 0.9513888888888888, "score": 427.0, "evalue": 7.98e-153, "description": "Internal stop codon at 339448. Nonsense mutation around 339448.", "psc": { "uniref90_id": "UniRef90_UPI0027E16080", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q50287", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0027E16080", "EC:3.1.21.3", "UniRef:UniRef50_Q50287", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_Q50287", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50287" ], "product": "Putative type I specificity subunit S.MpnORF201P" } }, "id": "CNOCLNGIAL_635", "locus": "GCA000143945_00600" }, { "type": "cds", "sequence": "contig_1", "start": 339523, "stop": 339810, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0AB33HM83", "UniRef:UniRef90_UPI0019133825" ], "nt": "TTGTTAGAAGTAAAGAACAAAAATAAATTTAGTTCCAAATTCTTATGTTTTGCACTAAAACTACAATCTAAAAAATTTGTGAATTATGCCTCTGCAATTCCGGTTCTAACAATAAAGAGAATTGCAGAAATCGAACTCTCCTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTTTGTTTGCATTTGAAAAACTTTGCAACGATTTAACCGAGGGGATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "a11db2ee396e898e6d3a89d9fdf70479", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 339223, "stop": 339819, "observations": { "mutation": [ 339448, 339808 ], "stop": [ 339448, 339808 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019133825", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 0.9890000000000001, "score": 184.0, "evalue": 1.83e-57, "gene_start": 1, "gene_end": 95, "reference_start": 108, "reference_end": 202, "reference_sequence": "KQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVTWTTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNWVQKIRN" }, "paralog": false, "identity": 0.98989898989899, "score": 397.0, "evalue": 8.83e-144, "description": "Internal stop codon at 339448, 339808. Nonsense mutation around 339448, 339808.", "psc": { "uniref90_id": "UniRef90_UPI0019133825", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AB33HM83", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019133825", "EC:3.1.21.3", "UniRef:UniRef50_A0AB33HM83", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HM83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HM83" ] } }, "id": "CNOCLNGIAL_636", "locus": "GCA000143945_00601" }, { "type": "cds", "sequence": "contig_1", "start": 339859, "stop": 340197, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ], "nt": "TTGGTCGAGGGTGGGTCATTTGGGAATATCTTTCCCCGTAGTCGTCGTCTGACTGCCACCCAGGGTGCCCTGGACATTCGCTTTGAGGCCAACGCCGACATTCGAAAAAGTGGGTGCGGGTTCGGTTCCGGATGCATTTTGCGATTGTTGTCCCTGGGTTGTTACCCCACTCGTACTACTGTTGCTCCCCCGGTGGTCATTCCCATACCTGTGCCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCGAGAACCGACCCCGCGAGGTTGAACCCGGTGGAGAAGCGCGTAAGCGTGGGGGAGAAGTGGGATCAGTTTGTGGTTTGCTGAACTAA", "aa": "MVEGGSFGNIFPRSRRLTATQGALDIRFEANADIRKSGCGFGSGCILRLLSLGCYPTRTTVAPPVVIPIPVPIPNPGNIIQHLVENRPREVEPGGEARKRGGEVGSVCGLLN", "aa_hexdigest": "b6549c27e0a4e4399c399fd53c909079", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "query_cov": 1.0357142857142858, "subject_cov": 0.8226950354609929, "identity": 0.578, "score": 105.0, "evalue": 6.22e-27, "valid": false, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11864.57919999999, "isoelectric_point": 9.241365623474117 }, "id": "CNOCLNGIAL_637", "locus": "GCA000143945_00602" }, { "type": "cds", "sequence": "contig_1", "start": 340555, "stop": 340881, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATAAGAGTGGCCAAGATGATTCCAACAAGTTCCAAGCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAGGTGTACGGTCTGAGTTCCACTCAAGATTCTAGCACCGGAGCCGGTGGCGCTGGAGGTAACACCGGAGGCGACACCAATACCCAGACTTATGCACGACCCGGAATCGGCTTTAAATTACCCTCCACGGACTCGGAATCTTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNKSGQDDSNKFQATDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVEVYGLSSTQDSSTGAGGAGGNTGGDTNTQTYARPGIGFKLPSTDSESSKATLITPGLA", "aa_hexdigest": "3599a4e0ed2bff56344026874b743ef7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11204.079199999984, "isoelectric_point": 4.654454231262207 }, "id": "CNOCLNGIAL_638", "locus": "GCA000143945_00603" }, { "type": "cds", "sequence": "contig_1", "start": 341114, "stop": 341335, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03257.18" ], "nt": "GTGTGGGATTTCAAGGGGGTGTGGCAGGATCAAGCTCGGTCCGACTCGCAAGCAGCTACAAGTACCACCACCGCAACGAAGGCTACCTTATCGGAGCACACCAATGCTTTGGCCTTTCAGGTGAGTTATACCGACCAGGATTCGTACAAGGCTTCCACTCAAAGCTCCGGCCAAAACCAAAACACCTCCCCCTACCTGCACTTGGTGCAGGGAAAAAAGTAG", "aa": "MWDFKGVWQDQARSDSQAATSTTTATKATLSEHTNALAFQVSYTDQDSYKASTQSSGQNQNTSPYLHLVQGKK", "aa_hexdigest": "94616c3ebb89eb4b96ee75d1db6d8df7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 31, "aa_cov": 0.3972602739726027, "hmm_cov": 1.0, "evalue": 3.251202310119906e-10, "score": 35.97065734863281, "start": 45, "stop": 73 } ], "seq_stats": { "molecular_weight": 8045.573400000003, "isoelectric_point": 6.699748420715332 }, "id": "CNOCLNGIAL_639", "locus": "GCA000143945_00604" }, { "type": "cds", "sequence": "contig_1", "start": 341374, "stop": 341592, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03257.18" ], "nt": "TTGGACCCCAACGAGGTTCGCGCCAAGCTGCGCCAAAGCTTTGGTACAGAAAATTCTACCCAACCCCAACCTAAAGCCCTCAAAACAACCACACCGGTATTTGGGACGAATAGTGGTAACCTCGGCAGTGTGCTTGGTGGTGGGGGTACCACGCAGGGCTCAGGCTCAGGTCAATCTGGTGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAA", "aa": "MDPNEVRAKLRQSFGTENSTQPQPKALKTTTPVFGTNSGNLGSVLGGGGTTQGSGSGQSGVDLSPVERVSGH", "aa_hexdigest": "5a981fa84af8ad3f9787c4ddc4f4a68c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 72, "aa_cov": 1.0, "hmm_cov": 0.9861111111111112, "evalue": 6.2361016457132225e-27, "score": 89.86980438232422, "start": 1, "stop": 72 } ], "seq_stats": { "molecular_weight": 7270.845000000002, "isoelectric_point": 8.275049018859864 }, "id": "CNOCLNGIAL_640", "locus": "GCA000143945_00605" }, { "type": "cds", "sequence": "contig_1", "start": 341912, "stop": 342436, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_287", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D866ED", "UniRef:UniRef100_P75490", "UniRef:UniRef50_P75490", "UniRef:UniRef90_P75490" ], "nt": "TTGCTGCCCACCTCTTTAAACACTTTTACGTTTTTCCAATTAATAATAAAGGAGGTCTTCACCATTAAACGCACAATTTCACTTACTTTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTATGACATGGGATTTAGTGACAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAATAATCAAAGGCTGATAAAGATTGAAACTACTTTAGCTACTCAAGGTGAACAATTAAATCAATTAATTAAAGTTGTTATTCTTCAGGGCGAACAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAAGGAGAACAAATAAAAGCTAAAGGAGAACAAATAAAAGCTCAAAGCGAGCAAATAAAAACTCAAGGAGAAACGCTTAAATTGATCCTACAAGCACTTGGAGGAATAAATAAGCGCTTAGACAAAGTTGATCCGCCTAAATAG", "aa": "MLPTSLNTFTFFQLIIKEVFTIKRTISLTLFKKRLNKDKINNCHVWEEELPDGSYDMGFSDNLNHMEKRKSGYVTQKQFSEFKDANNQRLIKIETTLATQGEQLNQLIKVVILQGEQIKELQVEQKAQGEQIKAKGEQIKAQSEQIKTQGETLKLILQALGGINKRLDKVDPPK", "aa_hexdigest": "4f27146806afe1ad4690dfc75aa0b72c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002D866ED", "uniref100_id": "UniRef100_P75490", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D866ED", "UniRef:UniRef100_P75490" ] }, "ips": { "uniref100_id": "UniRef100_P75490", "uniref90_id": "UniRef90_P75490" }, "psc": { "uniref90_id": "UniRef90_P75490", "product": "UPF0134 protein MPN_287", "uniref50_id": "UniRef50_P75490" }, "pscc": { "uniref50_id": "UniRef50_P75490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75490" ], "product": "UPF0134 protein MPN_287" }, "genes": [], "id": "CNOCLNGIAL_641", "locus": "GCA000143945_00606" }, { "type": "cds", "sequence": "contig_1", "start": 343033, "stop": 343674, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75595", "UniRef:UniRef90_A0AB38WA11" ], "nt": "ATGAAATTTAAGTATTGTGCCATCTTTTTCAGTGGCTTTCTAGGTCTTTCTGCCATCTTAGCTGCTTGTGGAGCGCGGGGCAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTTCTCTTTAACTTCAAAAAGTGCATCAAACGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCATATTGAAATTACCCAAATCGCTGGTGGTTATGATGGTGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAGGACACCACTAACTTTTACAACCTTATCTTGAATTATCCTGACTTAGTTTCTACTTTAGGTCGTGTTGGGATGGAACTACTGTTTGATAAAGTTAATACAGATAAATTATCCGACCGTTTCTTAGATTTCAATAAACGAATTAGTGCAATTTCTAAACCAGGGATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAAAAGTGCAAAAGGTGATAGTGACAATGTTAAAGTAAGTCAAAGGACTGGAGAAACTACTCAAAAAAGTAAAGTAACTAATCCTTTGAAAATTAATACTCAAAACGATCCTGCTACAAAAGATCTTTGA", "aa": "MKFKYCAIFFSGFLGLSAILAACGARGKFDQVDDGKIKLAFSLTSKSASNALQAIVKKYNEVKKPGDYHIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDL", "aa_hexdigest": "43c47b40f91aac03cb97426358e1305f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 343033, "stop": 343977, "observations": { "mutation": [ 343672, 343852, 343921 ], "stop": [ 343672, 343852, 343921 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38WA11", "query_cov": 1.0, "subject_cov": 0.44375, "identity": 1.0, "score": 415.0, "evalue": 1.13e-142, "gene_start": 1, "gene_end": 213, "reference_start": 1, "reference_end": 213, "reference_sequence": "MKFKYCAIFFSGFLGLSAILAACGARGKFDQVDDGKIKLAFSLTSKSASNALQAIVKKYNEVKKPGDYHIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDLWEKIEASAKANGKSNKDGQTKGKKKVEKSSSSSLVNLKQSTDQTTTDDGSQKSDNKIKESWGEYQEVDGGLKNFEFKASIFENWHDLLDFSTRVAKSFTNVKGKDIKKGTDIQGVLGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFGDNFIEFPRFTQEIKAPSTENGGQSNLLGTFEVKDSSKSKEVKKN" }, "paralog": false, "identity": 0.9904458598726115, "score": 606.0, "evalue": 5.02e-220, "description": "Internal stop codon at 343672, 343852, 343921. Nonsense mutation around 343672, 343852, 343921.", "psc": { "uniref90_id": "UniRef90_A0AB38WA11", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75595", "go_ids": [ "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38WA11", "UniRef:UniRef50_P75595", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75595" ], "product": "Uncharacterized lipoprotein MPN_097" } }, "id": "CNOCLNGIAL_642", "locus": "GCA000143945_00607" }, { "type": "cds", "sequence": "contig_1", "start": 343951, "stop": 344475, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTAAGTCGTTTACAAATGTAAAGGGAAAAGATATCAAAAAGGGAACTGATATCCAAGGTGTACTTGGTGTTGACAGTACGCCCAACTCATTATTCACCTCTGTTTTTGCAGCTGGTGGCGGTGATTACAATAATTTCTTCTACAAAATTGAAAACGGCCGTGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCCTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTTCAAAAGTTCCATCAATTAGCTTACAGTATTTCCTCTACTTCCGGTTTTTTCTATTCGTTTGCCGGTAACAGCGCTAAACGCTTAAAGTTTGGTGATAACTTTATTGAATTTCCTCGTTTTACTCAGGAAATTAAAGCACCTTCTACTGAAAATGGCGGCCAAAGCAATCTTTTAGGAACCTTTGAAGTTAAAGATAGCAGTAAATCTAAGGAAGTGAAAAAAAACTAA", "aa": "MAKSFTNVKGKDIKKGTDIQGVLGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFGDNFIEFPRFTQEIKAPSTENGGQSNLLGTFEVKDSSKSKEVKKN", "aa_hexdigest": "b0382547156ba35ae3570b57433b3939", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19158.103299999995, "isoelectric_point": 9.623598670959474 }, "id": "CNOCLNGIAL_643", "locus": "GCA000143945_00608" }, { "type": "cds", "sequence": "contig_1", "start": 344840, "stop": 345034, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGAGCAATCTAGCACATTACAAAGTGATGAAGCAATTGTGTTACCAGCTCCTGGTAAGTATCAAAGTGGTGACGCTAAGAAAGTAACCATCACCCAAGGTCCTAATATCATTGGCATTCACGCTAACGAAAAGGAAAACGCCGAAACGCAAAAGTTTGTTAACTGGTTTTTAAACAATACAGAAAGTTGA", "aa": "MLEQSSTLQSDEAIVLPAPGKYQSGDAKKVTITQGPNIIGIHANEKENAETQKFVNWFLNNTES", "aa_hexdigest": "2f9188835cec30a3efc4c7bc12902bb5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7032.7209, "isoelectric_point": 4.83730525970459 }, "id": "CNOCLNGIAL_644", "locus": "GCA000143945_00609" }, { "type": "cds", "sequence": "contig_1", "start": 345113, "stop": 345397, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTAAAAGAAATCTTTGACAAAGATAACAAAAAAGCTACTGAAAATAAGGACAATAATACTAGCAATAAAAAACAAGCTAATACCTATGCTGAAAAGGCGTTAGATTTGTTCCAACAAATTAGTAAAGGTGATATAGTTAGTTACAGCGACCCTAGCGATTTTAGAAGTGGTAAATTCCGCGATGGGATTGGCTCTAATTTTAACGCTGCAGTAAGTTCCAAAGCTGATTTTGACAGGTTTGTTAAAGGCTTCATCGCCACTTTGGGTAGTGAAATTTAA", "aa": "MPLKEIFDKDNKKATENKDNNTSNKKQANTYAEKALDLFQQISKGDIVSYSDPSDFRSGKFRDGIGSNFNAAVSSKADFDRFVKGFIATLGSEI", "aa_hexdigest": "ccda41382d0f2345a6eb88c671150457", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10433.430199999992, "isoelectric_point": 7.908417320251465 }, "id": "CNOCLNGIAL_645", "locus": "GCA000143945_00610" }, { "type": "cds", "sequence": "contig_1", "start": 345487, "stop": 345666, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGCAAATCAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAACAATCCAGGCGAATTCCCTGTTTATTCATCGGCTACAGTTAATAATGGTGAAATTGGCAGAATAAAAGATTGTGATCTTAAGGGTGAGTATGTGACTTGA", "aa": "MQIKTYKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGRIKDCDLKGEYVT", "aa_hexdigest": "586c91d0b4398bcdcba1cdfcc9a437c9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 60, "aa_cov": 0.9491525423728814, "hmm_cov": 0.9833333333333333, "evalue": 7.763869124855757e-11, "score": 37.9355354309082, "start": 4, "stop": 59 } ], "seq_stats": { "molecular_weight": 6724.738800000003, "isoelectric_point": 9.230019187927244 }, "id": "CNOCLNGIAL_646", "locus": "GCA000143945_00611" }, { "type": "cds", "sequence": "contig_1", "start": 345844, "stop": 346044, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGCAAGGAACTTTAGCAGAAATTGAGCTAGATTTCCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGTCTTCTATAGCGATTATTTACTTAACCCAAAAAATTGA", "aa": "MQGTLAEIELDFPSKKIQEKIATILDTFTELSAELSAELSAELSAELRERKKQYVFYSDYLLNPKN", "aa_hexdigest": "1b1e9f6bb6eb4ffe5b93109650ec09df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 41, "aa_cov": 0.6212121212121212, "hmm_cov": 0.975609756097561, "evalue": 4.4107175901346215e-07, "score": 25.719707489013672, "start": 3, "stop": 43 } ], "seq_stats": { "molecular_weight": 7569.504399999997, "isoelectric_point": 4.674973106384278 }, "id": "CNOCLNGIAL_647", "locus": "GCA000143945_00612" }, { "type": "cds", "sequence": "contig_1", "start": 346189, "stop": 346581, "strand": "+", "frame": 1, "gene": null, "product": "Putative type I specificity subunit S-MpnORF289P C-terminus", "db_xrefs": [ "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75487", "UniRef:UniRef90_P75487" ], "nt": "TTGGGTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGTCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTCCCTAAAGAAGAATTTGATATAAGGTTCTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAATATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCACCGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTCTTCCTGCTGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTTTTATTTAACTGA", "aa": "MGYSKTFRVEEKSITVSARGTIGVVFYRDFAYLPAVSLICFVPKEEFDIRFLFHALRAIKFKKQGSATGQLTVAQFKEYGIHVPSLKKQKEITAILDPLYSFFTDLNEGLPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "a78d9a67727d2af479b3a702a8ca12d5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75487", "query_cov": 1.0, "subject_cov": 0.896551724137931, "identity": 0.985, "score": 255.0, "evalue": 9.09e-86, "valid": true, "product": "Putative type I specificity subunit S.MpnORF289P C-terminus", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75487", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75487", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75487" ], "product": "Putative type I specificity subunit S.MpnORF289P C-terminus" }, "genes": [], "id": "CNOCLNGIAL_648", "locus": "GCA000143945_00613" }, { "type": "cds", "sequence": "contig_1", "start": 346619, "stop": 347209, "strand": "-", "frame": 2, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "COG:COG1214", "COG:J", "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_014325445.1", "SO:0001217", "UniParc:UPI0001DD651C", "UniRef:UniRef100_A0AAP8E4Y3", "UniRef:UniRef50_P47450", "UniRef:UniRef90_P75486" ], "nt": "ATGCGCTTTTTCAATAAGTACAAGCTCTTTTTGGACTGTGCTTACAAACACCTCAATATCGTACTGTTGGATTTTAAAACGAATACTGTGGTGGATCAACTAACAATACCAGTACAACAAAACCTCACGGAGTTGGCTGTGTATCACTTGGAAAAATTGTTAAAGAAAAACAAAGTACGCAATAACACTGTTAGACAGTTTTACGTAACCACTGGACCGGGTAGCTTTACTGGTCAACGCGTTGGTGCCATTATTGCTAAAACTTGGTGCACAGTAAACCCTAACTGTCAACTCTTTGCTTTAAACTCACTCCGCCTTCAAATTCCTTATGGCTGTGGCATTAGCAAAATTAGTGCTGGCAATGAAAAGAACTACTGTGGTTTGTTTACCGAAACTACGAGTGAAATAGCACTCTTAGCTAAACCAGATTTTGTCAAGCTGTGCAAAGCAAATACCGAGTTACCGTTATATGAAAACTTTGAAAACATCGACAGTATTGAGGAGTTATTCCTAAACAACATCGAACGGTTTGAACTAGTGGAAAATCCCCAAAATTTAGAACTACTCTACCTTAAAGATCCCGTTAATTAG", "aa": "MRFFNKYKLFLDCAYKHLNIVLLDFKTNTVVDQLTIPVQQNLTELAVYHLEKLLKKNKVRNNTVRQFYVTTGPGSFTGQRVGAIIAKTWCTVNPNCQLFALNSLRLQIPYGCGISKISAGNEKNYCGLFTETTSEIALLAKPDFVKLCKANTELPLYENFENIDSIEELFLNNIERFELVENPQNLELLYLKDPVN", "aa_hexdigest": "4637762e47aa82473716d0c7947bb369", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD651C", "ncbi_nrp_id": "WP_014325445.1", "uniref100_id": "UniRef100_A0AAP8E4Y3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD651C", "RefSeq:WP_014325445.1", "UniRef:UniRef100_A0AAP8E4Y3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E4Y3", "uniref90_id": "UniRef90_P75486" }, "psc": { "uniref90_id": "UniRef90_P75486", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P47450", "cog_id": "COG1214", "cog_category": "J", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_P47450", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47450" ], "product": "Uncharacterized protein MG208" }, "genes": [ "tsaB" ], "id": "CNOCLNGIAL_649", "locus": "GCA000143945_00614" }, { "type": "cds", "sequence": "contig_1", "start": 347193, "stop": 347543, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGAACAGTTCTAACAAGGCCAAGAATGCTTTTACTAAGTTTACGGTGTTAAACCAAAACGAAAAGGCTGCTTTAATTAAGTGTGAACTGTTAACCGGACGAACTCACCAAATTAGGGTACACTTGCAGTTTATTAAACACCCCGTTTACAACGATCCCTTATACGGTTTGAAGGGTGAGCAAGCTACTGAATATGGCCAGTATTTACACGCGCAACAAATTAGTTTTATCCACCCCACTTTGAATAAGGAAATGGGTTTTGAAGCACAGTTAGATAAAACTTTTAGCGATAAGTTAGATAATTTAAACTTGAAAATTGCTAATTCCCTTTATGCGCTTTTTCAATAA", "aa": "MGNSSNKAKNAFTKFTVLNQNEKAALIKCELLTGRTHQIRVHLQFIKHPVYNDPLYGLKGEQATEYGQYLHAQQISFIHPTLNKEMGFEAQLDKTFSDKLDNLNLKIANSLYALFQ", "aa_hexdigest": "384f69b6fa9ffd4eb57c45f6ee9075d6", "start_type": "GTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13256.989299999987, "isoelectric_point": 9.015726280212402 }, "id": "CNOCLNGIAL_650", "locus": "GCA000143945_00615" }, { "type": "cds", "sequence": "contig_1", "start": 347547, "stop": 347972, "strand": "-", "frame": 1, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG209-like protein", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0120159", "KEGG:K06180", "SO:0001217", "UniRef:UniRef50_P47451", "UniRef:UniRef90_P75485" ], "nt": "GTGCAACCAGAAGACCGGGTACACGTTAATTGGAGTGAGGAACTGTTTGAAAAAGTGCCAGTTGAAGTCCAGCCCTATGACTTTCCCTTGGACATCTTATATGAAGATGAGCAAATTATGGTGGTCAATAAACCCAATGGTTTAATTAGCCACCCGACTAGTTTTAATGAAAGTGAAAGTTTACTGGGTGCCGCTTTGTTTCACTGCAACCACCAACCGGTGTTTTTAGTCCACCGCCTAGACCGTGATACGAGTGGTGTGATTATGCTAGCGAAAAACCAAAGTAGCTTGCTGCACTTGCAAAAACAGCTCCAGCAACGGGTTATGAAGCGTTATTACTTAGCTTTAGTCCATTTTCCCCTAGACAGTTTGTCTGGTACCATTAGCGCCCCGTTAGAACGAGTGGGTAACAATAAGGTCATGTGA", "aa": "MQPEDRVHVNWSEELFEKVPVEVQPYDFPLDILYEDEQIMVVNKPNGLISHPTSFNESESLLGAALFHCNHQPVFLVHRLDRDTSGVIMLAKNQSSLLHLQKQLQQRVMKRYYLALVHFPLDSLSGTISAPLERVGNNKVM", "aa_hexdigest": "f4b07f53c49b25a332940f18f3a24ad4", "start_type": "GTG", "rbs_motif": null, "genes": [ "rluA" ], "truncated": "both", "pseudogene": { "start": 347244, "stop": 348122, "observations": { "mutation": [ 347549, 347978 ], "stop": [ 347549, 347978 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75485", "query_cov": 1.0, "subject_cov": 0.45483870967741935, "identity": 0.993, "score": 282.0, "evalue": 5.52e-94, "gene_start": 1, "gene_end": 141, "reference_start": 51, "reference_end": 191, "reference_sequence": "MEQTFSVTTAQRLDTFLATLLNLSRVKVAKLIVDGLVSVNGKKITKNGWLVQPEDRVHVNWSEELFEKVPVEVQPYDFPLDILYEDEQIMVVNKPNGLISHPTSFNESESLLGAALFHCNHQPVFLVHRLDRDTSGVIMLAKNQSSLLHLQKQLQQRVMKRYYLALVHFPLDSLSGTISAPLERVGNNKVMWKVGNSSNKAKNAFTKFTVLNQNEKAALIKXCELLTGRTHQIRVHLQFIKHPVYNDPLYGLKSEQATEYGQYLHAQQISFIHPTLNKEMGFEAQLDKTFSDKLDNLNLKIANSLYALFQ" }, "paralog": false, "identity": 0.9863481228668942, "score": 568.0, "evalue": 3.52e-209, "description": "Internal stop codon at 347549, 347978. Nonsense mutation around 347549, 347978.", "psc": { "uniref90_id": "UniRef90_P75485", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG209 homolog", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47451", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180", "go_ids": [ "GO:0003723", "GO:0120159", "GO:0000455" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75485", "EC:5.4.99.-", "UniRef:UniRef50_P47451", "COG:COG0564", "COG:J", "KEGG:K06180", "GO:0003723", "GO:0120159", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47451", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47451" ], "product": "Uncharacterized RNA pseudouridine synthase MG209" } }, "id": "CNOCLNGIAL_651", "locus": "GCA000143945_00616" }, { "type": "cds", "sequence": "contig_1", "start": 347976, "stop": 348122, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01479.30" ], "nt": "ATGGAACAAACTTTTAGTGTTACTACAGCCCAACGCTTAGATACCTTTTTGGCCACACTACTCAACCTTTCCCGGGTTAAAGTAGCCAAGTTAATTGTCGATGGTTTGGTGAGTGTTAACGGTAAAAAAATTACTAAAAATGGTTGA", "aa": "MEQTFSVTTAQRLDTFLATLLNLSRVKVAKLIVDGLVSVNGKKITKNG", "aa_hexdigest": "611db4e9f4b8d7087542eda5e7a7ab1c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01479.30", "name": "S4", "length": 38, "aa_cov": 0.7708333333333334, "hmm_cov": 1.0, "evalue": 9.187555224809536e-12, "score": 40.05647277832031, "start": 11, "stop": 47 } ], "seq_stats": { "molecular_weight": 5220.094100000002, "isoelectric_point": 10.165906715393067 }, "id": "CNOCLNGIAL_652", "locus": "GCA000143945_00617" }, { "type": "cds", "sequence": "contig_1", "start": 348122, "stop": 348223, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAACTTAGCGGACTGTTGCATTACCTTTAGTTTTATTGGTCTGTTCCTCTCCTTTTTAATTCAGTTCTTCAAAGAAATGAAGCAAACTAAATCTTAA", "aa": "MFNLADCCITFSFIGLFLSFLIQFFKEMKQTKS", "aa_hexdigest": "54ad894d5bc253b4bf89f0e5464f84d7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3899.661100000001, "isoelectric_point": 7.799981498718263 }, "id": "CNOCLNGIAL_653", "locus": "GCA000143945_00618" }, { "type": "cds", "sequence": "contig_1", "start": 348326, "stop": 348676, "strand": "-", "frame": 2, "gene": "lspA", "product": "signal peptidase II", "db_xrefs": [ "COG:COG0597", "COG:MU", "EC:3.4.23.36", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306", "KEGG:K03101", "SO:0001217", "UniRef:UniRef50_P75484", "UniRef:UniRef90_P75484" ], "nt": "ATGGCAAAAGCCCCAACTTTTTTTTCAAAATTGCTAAAGCAAATCCTTTTTGCTAACCGCAAACCCTTTTTGTACTATAAGCTTGCCTTAATCCTGTTTGTCGGTTTTGTCATTTTGTTTCAGGTATTTATGCTGCGTGCTGCCTTAAATGGTGAGAAGGGTATTAATGGTGCTAATGGTACAGATGTAGCTCGCAGTTCCTTTATTAGTATCTATGTTATCGGTAACAAGGGGGTTGGTTTTAGCTTATTGGCAGACCAACCAGGACTTGTTTACTTTCTCCAGGGTTTTCTATCTTTCATAGCACTGTTCTTTTTGGTTTTCTCTACTAGTTACAACTACATCTTTTGA", "aa": "MAKAPTFFSKLLKQILFANRKPFLYYKLALILFVGFVILFQVFMLRAALNGEKGINGANGTDVARSSFISIYVIGNKGVGFSLLADQPGLVYFLQGFLSFIALFFLVFSTSYNYIF", "aa_hexdigest": "a2ce7117ea06a98a81dda82195433baf", "start_type": "ATG", "rbs_motif": null, "genes": [ "lspA" ], "truncated": "5-prime", "pseudogene": { "start": 348122, "stop": 348676, "observations": { "mutation": [ 348328 ], "stop": [ 348328 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75484", "query_cov": 1.0, "subject_cov": 0.6304347826086957, "identity": 1.0, "score": 217.0, "evalue": 2.3e-70, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MAKAPTFFSKLLKQILFANRKPFLYYKLALILFVGFVILFQVFMLRAALNGEKGINGANGTDVARSSFISIYVIGNKGVGFSLLADQPGLVYFLQGFLSFIALFFLVFSTSYNYIFWITTLAFGSLGNFFDRLTSGSGEVLDYFVFSGGNSVFNLADCCITFSFIGLFLSFLIQFFKEMKQTKS" }, "paralog": false, "identity": 0.9945652173913043, "score": 359.0, "evalue": 6.65e-129, "description": "Internal stop codon at 348328. Nonsense mutation around 348328.", "psc": { "uniref90_id": "UniRef90_P75484", "gene": "lspA", "product": "signal peptidase II", "ec_ids": [ "3.4.23.36" ], "uniref50_id": "UniRef50_P75484", "cog_id": "COG0597", "cog_category": "MU", "kegg_orthology_id": "K03101", "go_ids": [ "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75484", "EC:3.4.23.36", "UniRef:UniRef50_P75484", "COG:COG0597", "COG:MU", "KEGG:K03101", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ] }, "pscc": { "uniref50_id": "UniRef50_P75484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75484" ], "product": "Lipoprotein signal peptidase" } }, "id": "CNOCLNGIAL_654", "locus": "GCA000143945_00619" }, { "type": "cds", "sequence": "contig_1", "start": 348715, "stop": 349335, "strand": "+", "frame": 1, "gene": "yajL", "product": "putative protein MPN_294", "db_xrefs": [ "COG:COG0693", "COG:V", "RefSeq:WP_010874651.1", "SO:0001217", "UniParc:UPI000013A574", "UniRef:UniRef100_P75483", "UniRef:UniRef50_P75483", "UniRef:UniRef90_P75483" ], "nt": "ATGGATTTGCTTACAACCGCAAACAAAACGCCGCGAATCGCCAAATCACGTCCTGTTCGAATTGCCATCATTATAACGCAGAAGACTAATGATCTGCACGCTACTGTACCCTGCTTTTTATGGCGCAAAGCAAGGTATGCTGTTGACCTGATTTCCGCTGAAGCCAAAGCTAGCATTATGTTAGAGATGGGGATTCATGTGCGCTGTGACAACACTTTGTCCAAAACTAACTTTAACCAGTATACCGCTGCTTTTATTCCCCATGGCAACACTACTAGGTTGGTGGAAATAGACAAACTAAGAAAGGACTTGGAGAAGTTTGTCTATAAACCCAAGGGACCAATGCGCTGGTTGTTTAGCAGTGGTAATGGTGCTTGTGTTTTGAAGGAGTTTGACTTGATTGCCCCTGACCAATTAGTCACCGTTCAAAACGAAAAGGAAATGGTTAAACTTTTGGGAAAAAATTTCATCAAGCAACCAGTACATGTGGACAAAAACATTATTAGTTGTGCCAATTCTTGTGGCTTGACTAAATTCTCCTTTAAGGTAATTGAAGAGCTTTCAGGAATAGAACTAGCGCGCAAAACGGCGAATCTTGTTGACCATATCTACAAAGGATAA", "aa": "MDLLTTANKTPRIAKSRPVRIAIIITQKTNDLHATVPCFLWRKARYAVDLISAEAKASIMLEMGIHVRCDNTLSKTNFNQYTAAFIPHGNTTRLVEIDKLRKDLEKFVYKPKGPMRWLFSSGNGACVLKEFDLIAPDQLVTVQNEKEMVKLLGKNFIKQPVHVDKNIISCANSCGLTKFSFKVIEELSGIELARKTANLVDHIYKG", "aa_hexdigest": "8eb1170c0b77bc3c543ae945ca8b91cc", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI000013A574", "ncbi_nrp_id": "WP_010874651.1", "uniref100_id": "UniRef100_P75483", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A574", "RefSeq:WP_010874651.1", "UniRef:UniRef100_P75483" ] }, "ips": { "uniref100_id": "UniRef100_P75483", "uniref90_id": "UniRef90_P75483" }, "psc": { "uniref90_id": "UniRef90_P75483", "gene": "YajL", "product": "Uncharacterized protein MPN_294", "uniref50_id": "UniRef50_P75483", "cog_id": "COG0693", "cog_category": "V" }, "pscc": { "uniref50_id": "UniRef50_P75483", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75483" ], "product": "Uncharacterized protein MPN_294" }, "genes": [ "yajL" ], "id": "CNOCLNGIAL_655", "locus": "GCA000143945_00620" }, { "type": "cds", "sequence": "contig_1", "start": 349597, "stop": 349743, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCACTAAGCAAATTAAAGCTGGCAGTGTTAACGAAGTGTTAGCGCAAAAATTAGTCCAACTCGATGCGCTGCGCACTAACTATCTCATTGGCGCTTTGTACTCCACCAATAAAACACAAAAGAAGCTGTTTAAGCAATCGTGA", "aa": "MFTKQIKAGSVNEVLAQKLVQLDALRTNYLIGALYSTNKTQKKLFKQS", "aa_hexdigest": "9baa1dc809fb450e3e5ff1f7f9ef57b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5430.325400000001, "isoelectric_point": 10.09466915130615 }, "id": "CNOCLNGIAL_656", "locus": "GCA000143945_00621" }, { "type": "cds", "sequence": "contig_1", "start": 349786, "stop": 350046, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTTAGTCGGTTTGCCGTTTGAAAAAGGGGAATATCATAAGCAACTGTATGCGCATTCCCGTCAAGAGATTTTGGATCTAGTGGAAGCGGTCAAAAAGCTGTATCTCAGACCAGAAAAGGATGACAAACTGGAGTTTGCTGATTCTTCCAAGGTAAGTGAGTCTAAGAGTATTAAGGTAACCAATGCAGTAACCTTACCAAGTGATGATTTAGATAAAGAGCTGTTTGAATTTAGCGGGGAAGGGGGTGATGAATAA", "aa": "MTLVGLPFEKGEYHKQLYAHSRQEILDLVEAVKKLYLRPEKDDKLEFADSSKVSESKSIKVTNAVTLPSDDLDKELFEFSGEGGDE", "aa_hexdigest": "5d5f7dade4ba0bf05da7074ab771a95f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9733.791899999997, "isoelectric_point": 4.793652915954591 }, "id": "CNOCLNGIAL_657", "locus": "GCA000143945_00622" }, { "type": "cds", "sequence": "contig_1", "start": 350046, "stop": 350228, "strand": "+", "frame": 3, "gene": "rpsU", "product": "30S ribosomal protein S21", "db_xrefs": [ "COG:COG0828", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_010874653.1", "SO:0001217", "UniParc:UPI0000134E7D", "UniRef:UniRef100_P57079", "UniRef:UniRef50_Q8EUK4", "UniRef:UniRef90_P57079" ], "nt": "ATGCCAAAGATTGAAGTTAAAAACGATGATTTAGAGTTAGCTTTAAAAAAGTTTAAACGGGTTTCGCTAGAAATTCGCCGTTTAGCACAGCGTCACGAATACCACCTTCGAAAAGGAATGCGCTTGCGTGAAAAGCGAAAGATTGCGCAGAAGAAACGCCGTAAGTTTCGTAATATGGTCTAG", "aa": "MPKIEVKNDDLELALKKFKRVSLEIRRLAQRHEYHLRKGMRLREKRKIAQKKRRKFRNMV", "aa_hexdigest": "225e633bab1c8eb7c1d04d1005d5a625", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E7D", "ncbi_nrp_id": "WP_010874653.1", "uniref100_id": "UniRef100_P57079", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E7D", "RefSeq:WP_010874653.1", "UniRef:UniRef100_P57079" ] }, "ips": { "uniref100_id": "UniRef100_P57079", "uniref90_id": "UniRef90_P57079" }, "psc": { "uniref90_id": "UniRef90_P57079", "gene": "rpsU", "product": "30S ribosomal protein S21", "uniref50_id": "UniRef50_Q8EUK4", "cog_id": "COG0828", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EUK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EUK4" ], "product": "Small ribosomal subunit protein bS21" }, "genes": [ "rpsU" ], "id": "CNOCLNGIAL_658", "locus": "GCA000143945_00623" }, { "type": "cds", "sequence": "contig_1", "start": 350253, "stop": 350702, "strand": "+", "frame": 3, "gene": null, "product": "putative MG211-like protein", "db_xrefs": [ "RefSeq:WP_014325447.1", "SO:0001217", "UniParc:UPI0001DD651E", "UniRef:UniRef100_A0AAX0SQI7", "UniRef:UniRef50_P47453", "UniRef:UniRef90_P75481" ], "nt": "ATGGCTAACGATAAAAAGGATAAGATTATTTTGAGTGGTGAGTTAACTAACCATCGCTTTAATTTTACCAAGGATGGGGAAAACGGCTATAGTGCTTATGAAGTAGACCGTTTTCTAGATCAGCTAGTTCACACTTTAACCCATTACGAAGCGCAGCGTAACCGTGAGGAGGAGATGAAAACGGCTTACGAAAAGCTCTTTCAAGACCGCGACGAAATTTTAAAGCGTTGCTCCAAACTAGAAGCAGAGCTCAATAACTTTTATGAAAACGGTTATTCCAACCGTGTGTTAATTAGTCGGGTTCAAGCCTTGGAAAACAAGATCGAAAGCCTCCCTAGTGGTCAAAATGACCGTCTAGAGCGCATCGAAAAGTTGCTAAAAAGGGTGATTAAGCACTGGACTGACGGGGAGGATTTATCCTATGGTGACTTCGATGATGATTTCTTTTAA", "aa": "MANDKKDKIILSGELTNHRFNFTKDGENGYSAYEVDRFLDQLVHTLTHYEAQRNREEEMKTAYEKLFQDRDEILKRCSKLEAELNNFYENGYSNRVLISRVQALENKIESLPSGQNDRLERIEKLLKRVIKHWTDGEDLSYGDFDDDFF", "aa_hexdigest": "a9346c4cb76ffb286b164bf8d335ef4a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD651E", "ncbi_nrp_id": "WP_014325447.1", "uniref100_id": "UniRef100_A0AAX0SQI7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD651E", "RefSeq:WP_014325447.1", "UniRef:UniRef100_A0AAX0SQI7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQI7", "uniref90_id": "UniRef90_P75481" }, "psc": { "uniref90_id": "UniRef90_P75481", "product": "Uncharacterized protein MG211 homolog", "uniref50_id": "UniRef50_P47453" }, "pscc": { "uniref50_id": "UniRef50_P47453", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47453" ], "product": "Uncharacterized protein MG211" }, "genes": [], "id": "CNOCLNGIAL_659", "locus": "GCA000143945_00624" }, { "type": "cds", "sequence": "contig_1", "start": 350705, "stop": 351055, "strand": "+", "frame": 2, "gene": "acpS", "product": "holo-ACP synthase", "db_xrefs": [ "COG:COG0736", "COG:I", "EC:2.7.8.7", "GO:0000287", "GO:0005737", "GO:0006633", "GO:0008897", "SO:0001217", "UniRef:UniRef50_P75480", "UniRef:UniRef90_P75480" ], "nt": "ATGATTCTAGGCATAGGGATTGATTTAGTCGAAATCAAACGCTTTGAACAATTAGCTAGACAAACTGATAACTGTTTTGCGAAACGCTTGTTGACTTCAACGGAGTACGCGCACTATGCGAAGCTGCGTAAAGATAGTGAAAAGTCGAGTTTTTTAGCAGTCCACTGGTCATTAAAAGAAGCGATTTATAAGGCTGTTAACCACATCAAGCCATTGTTTAGCCAACTGGAAATTACAAAGAAAAACCAACGTTATAACTGCCAGATTGACCCTAAGATTGAACTTTTGCTCTCGGTTAGTTACAGCAGTAATAACATTACTGCCATCTGTTTGGCACAACAAACACCATGA", "aa": "MILGIGIDLVEIKRFEQLARQTDNCFAKRLLTSTEYAHYAKLRKDSEKSSFLAVHWSLKEAIYKAVNHIKPLFSQLEITKKNQRYNCQIDPKIELLLSVSYSSNNITAICLAQQTP", "aa_hexdigest": "6e0ae46a45c7e4b65b3016037358e773", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75480", "query_cov": 1.0, "subject_cov": 0.9747899159663865, "identity": 1.0, "score": 230.0, "evalue": 1.76e-76, "valid": true, "gene": "acpS", "product": "holo-ACP synthase", "ec_ids": [ "2.7.8.7" ], "uniref50_id": "UniRef50_P75480", "cog_id": "COG0736", "cog_category": "I", "go_ids": [ "GO:0005737", "GO:0008897", "GO:0000287", "GO:0006633" ] }, "pscc": { "uniref50_id": "UniRef50_P75480", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75480" ], "product": "Holo-[acyl-carrier-protein] synthase" }, "genes": [ "acpS" ], "id": "CNOCLNGIAL_660", "locus": "GCA000143945_00625" }, { "type": "cds", "sequence": "contig_1", "start": 351052, "stop": 351429, "strand": "+", "frame": 1, "gene": "plsC", "product": "putative 1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "EC:2.3.1.51", "GO:0003841", "GO:0006654", "GO:0016020", "GO:0016024", "SO:0001217", "UniRef:UniRef50_Q49402", "UniRef:UniRef90_P75479" ], "nt": "ATGAAAAAATTAACACAAGCTTTTTTGCGTTTTTGTCTGAGATTCTTACAACTGCTCAGTTTAGTTTTGGTTTTACCCGTTTTTGTGTTAATGCTCATCTCTAGCCTAATTAGTGCTAAAAATTATGAAAGCATCCCGGAAAACTATCCCCCAGAAATCCGCTTTAAGAAGGTGTACCGCTTGGTATCACTTTTCCTCTACATTAAAGGAGTTAAAGTGGTTATTGTTAACCCTGAAAATGTGCCTAAAAAGGCTGTTTTAGTGGTAGCTAATCATAAGTCCAATTTAGATCCCCTCATCCTCATTAAGGCCTTTGGTAAAACTGAAGGGGTACCACCGTTAACCTTTATTGCCAAGATTGAACTGCAAGATACCTGA", "aa": "MKKLTQAFLRFCLRFLQLLSLVLVLPVFVLMLISSLISAKNYESIPENYPPEIRFKKVYRLVSLFLYIKGVKVVIVNPENVPKKAVLVVANHKSNLDPLILIKAFGKTEGVPPLTFIAKIELQDT", "aa_hexdigest": "49f75760ef28420f696218f266615553", "start_type": "ATG", "rbs_motif": null, "genes": [ "plsC" ], "truncated": "3-prime", "pseudogene": { "start": 351052, "stop": 351732, "observations": { "mutation": [ 351427 ], "stop": [ 351427 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75479", "query_cov": 1.0, "subject_cov": 0.4699248120300752, "identity": 1.0, "score": 228.0, "evalue": 1.28e-73, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MKKLTQAFLRFCLRFLQLLSLVLVLPVFVLMLISSLISAKNYESIPENYPPEIRFKKVYRLVSLFLYIKGVKVVIVNPENVPKKAVLVVANHKSNLDPLILIKAFGKTEGVPPLTFIAKIELQDTWLFKIMKLIDCVFIDRKNLRQMAASLEQQQQIIRQGTALCVFPEGTRVLSRQIGEFKSGALKVAYNAFVPIVPLTIVGSMGHMESKKRLQKAQVERDRGYKIQVIFNTPINPINFNQIDSQNVANNVWREISQTYAQYCQD" }, "paralog": false, "identity": 0.995575221238938, "score": 434.0, "evalue": 7.5e-157, "description": "Internal stop codon at 351427. Nonsense mutation around 351427.", "psc": { "uniref90_id": "UniRef90_P75479", "gene": "plsC", "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase", "ec_ids": [ "2.3.1.51" ], "uniref50_id": "UniRef50_Q49402", "cog_id": "COG0204", "cog_category": "I", "go_ids": [ "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75479", "EC:2.3.1.51", "UniRef:UniRef50_Q49402", "COG:COG0204", "COG:I", "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ] }, "pscc": { "uniref50_id": "UniRef50_Q49402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49402" ], "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "CNOCLNGIAL_661", "locus": "GCA000143945_00626" }, { "type": "cds", "sequence": "contig_1", "start": 351442, "stop": 351852, "strand": "+", "frame": 1, "gene": "plsC", "product": "putative 1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "EC:2.3.1.51", "GO:0003841", "GO:0006654", "GO:0016020", "GO:0016024", "SO:0001217", "UniRef:UniRef50_Q49402", "UniRef:UniRef90_P75479" ], "nt": "ATGAAGCTGATTGACTGTGTCTTTATTGATCGAAAAAACCTGCGTCAAATGGCAGCATCCTTGGAACAACAGCAGCAAATTATCCGCCAGGGCACTGCTCTGTGTGTTTTTCCTGAGGGTACACGGGTACTCTCTAGGCAAATTGGTGAGTTTAAATCGGGCGCTTTAAAGGTCGCTTACAACGCCTTTGTGCCGATTGTGCCACTAACGATTGTGGGTAGCATGGGACACATGGAGTCCAAAAAGCGTTTACAAAAAGCCCAGGTAGAGCGCGATCGTGGTTATAAAATCCAGGTCATCTTTAACACACCTATTAACCCAATTAACTTTAACCAGATTGATTCGCAAAATGTGGCTAATAATGTCTGGCGTGAAATTAGTCAAACTTATGCGCAATATTGCCAAGACTAG", "aa": "MKLIDCVFIDRKNLRQMAASLEQQQQIIRQGTALCVFPEGTRVLSRQIGEFKSGALKVAYNAFVPIVPLTIVGSMGHMESKKRLQKAQVERDRGYKIQVIFNTPINPINFNQIDSQNVANNVWREISQTYAQYCQD", "aa_hexdigest": "5b0247043a500f3e2c5ff4c822c7bc1a", "start_type": "ATG", "rbs_motif": null, "genes": [ "plsC" ], "truncated": "5-prime", "pseudogene": { "start": 351142, "stop": 351852, "observations": { "mutation": [ 351427 ], "stop": [ 351427 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75479", "query_cov": 1.0, "subject_cov": 0.5112781954887218, "identity": 1.0, "score": 268.0, "evalue": 4.49e-89, "gene_start": 1, "gene_end": 136, "reference_start": 131, "reference_end": 266, "reference_sequence": "MKKLTQAFLRFCLRFLQLLSLVLVLPVFVLMLISSLISAKNYESIPENYPPEIRFKKVYRLVSLFLYIKGVKVVIVNPENVPKKAVLVVANHKSNLDPLILIKAFGKTEGVPPLTFIAKIELQDTWLFKIMKLIDCVFIDRKNLRQMAASLEQQQQIIRQGTALCVFPEGTRVLSRQIGEFKSGALKVAYNAFVPIVPLTIVGSMGHMESKKRLQKAQVERDRGYKIQVIFNTPINPINFNQIDSQNVANNVWREISQTYAQYCQD" }, "paralog": false, "identity": 0.9957627118644068, "score": 461.0, "evalue": 1.26e-167, "description": "Internal stop codon at 351427. Nonsense mutation around 351427.", "psc": { "uniref90_id": "UniRef90_P75479", "gene": "plsC", "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase", "ec_ids": [ "2.3.1.51" ], "uniref50_id": "UniRef50_Q49402", "cog_id": "COG0204", "cog_category": "I", "go_ids": [ "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75479", "EC:2.3.1.51", "UniRef:UniRef50_Q49402", "COG:COG0204", "COG:I", "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ] }, "pscc": { "uniref50_id": "UniRef50_Q49402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49402" ], "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "CNOCLNGIAL_662", "locus": "GCA000143945_00627" }, { "type": "cds", "sequence": "contig_1", "start": 351976, "stop": 352305, "strand": "+", "frame": 1, "gene": null, "product": "dihydrofolate reductase", "db_xrefs": [ "EC:1.5.1.3", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_UPI000A8432F8", "UniRef:UniRef90_UPI000A8432F8" ], "nt": "ATGGGTTACAGCACCTTTCAGGAGTTAGGTGACAAGCTAACTGATCACAACGTGGTGGTGTTGAGTAAAAAACACTTTGAAGAATTGCAAAACAACACCAACATTAAGGTGTTCAATTCAATTGAAAAGCTCTTGCAACACCACTTTAACCGTGATTTGTATGTGATTGGGGGCAAGCAAATCTTCCACCACTTTATTGAACTGGCTGATCGTTTAATTATTTCGGTTTTACCAGTGGACTTTAAGTGTAACCTTCGCTTAAAGTTGGGCTTGGACAGCTTTGAATTAATGCAAGAACAACAACACAGCCAATTTAAGGTGCAGTACTGA", "aa": "MGYSTFQELGDKLTDHNVVVLSKKHFEELQNNTNIKVFNSIEKLLQHHFNRDLYVIGGKQIFHHFIELADRLIISVLPVDFKCNLRLKLGLDSFELMQEQQHSQFKVQY", "aa_hexdigest": "4758eccfab93aec56dc3de88ed4bac64", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 351853, "stop": 352390, "observations": { "insertion": [ 352357 ], "mutation": [ 351916, 352303 ], "stop": [ 351916, 352303 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A8432F8", "query_cov": 1.0, "subject_cov": 0.6089385474860335, "identity": 0.991, "score": 219.0, "evalue": 1.25e-71, "gene_start": 1, "gene_end": 109, "reference_start": 42, "reference_end": 150, "reference_sequence": "MITLIWCQDKHFGIGRDNTIPWKLTEANQHFYNTTKNQTVVMGYNTFQELGDKLTDHNVVVLSKKHFEELQNNTNIKVFNSIEKLLQHHFNRDLYVIGGKQIFHHFIELADRLIISVLPVDFKCNLRLKLGLDSFELMQEQQHSQFKVQYWHKKHPERLSFNVFLEDYXMGLYQTCWNF" }, "paralog": false, "identity": 0.9776536312849162, "score": 355.0, "evalue": 2.87e-127, "description": "Internal stop codon at 351916, 352303. Frameshift due to insertion around 352357. Nonsense mutation around 351916, 352303.", "psc": { "uniref90_id": "UniRef90_UPI000A8432F8", "product": "dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_UPI000A8432F8", "kegg_orthology_id": "K00287", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A8432F8", "EC:1.5.1.3", "UniRef:UniRef50_UPI000A8432F8", "KEGG:K00287" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A8432F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A8432F8" ], "product": "dihydrofolate reductase" } }, "id": "CNOCLNGIAL_663", "locus": "GCA000143945_00628" }, { "type": "cds", "sequence": "contig_1", "start": 352345, "stop": 352836, "strand": "+", "frame": 1, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "KEGG:K05896", "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF", "UniRef:UniRef90_UPI000AF5CFDF" ], "nt": "TTGGAAGATTACAATGGGACTTTACCAAACTTGCTGGAACTTTTAATTGATAAGAAGTTCAACTTGCACCAGGTCGATATTGCCAAAATTACGACACAATACCTCCACTTAATTAACACTAACTTAAATAAACAAGCAATTGAACCAATTACCGACTACCTTGTCATTACTAGTCGAATTGTGGAGCAGAAGGCCAATAACTTACTGCAAATTAATGATATTGCCCTAGACTCTGACTTTTTAGACAACAAGCTGCGTGATAAACTAGTAGCACAGTTAGTGGAGTACAAACGTTACCGTGAATCACTCGATGACTTTGAGAAGCTAAGGATCAATCGCTTAGCTTACTTTTCCAAAGATAATGACTTTAACCGCTTTATCCAAACAGTTGACAAGAGTAACACAGAACCCGTGAAAATTGAGGACGAGCTACCCAATTATGTAAGTGTCTTAAAGTTGCATCACGCCATGAATAAGCTAATGCAGCGCTGA", "aa": "MEDYNGTLPNLLELLIDKKFNLHQVDIAKITTQYLHLINTNLNKQAIEPITDYLVITSRIVEQKANNLLQINDIALDSDFLDNKLRDKLVAQLVEYKRYRESLDDFEKLRINRLAYFSKDNDFNRFIQTVDKSNTEPVKIEDELPNYVSVLKLHHAMNKLMQR", "aa_hexdigest": "a1dd2b5742512480cabdfbcb33583015", "start_type": "TTG", "rbs_motif": null, "genes": [ "scpA" ], "truncated": "3-prime", "pseudogene": { "start": 352375, "stop": 353139, "observations": { "mutation": [ 352834 ], "stop": [ 352834 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "query_cov": 0.9386503067484663, "subject_cov": 0.4608433734939759, "identity": 0.993, "score": 299.0, "evalue": 8.16e-100, "gene_start": 11, "gene_end": 163, "reference_start": 1, "reference_end": 153, "reference_sequence": "MLELLIDKKFNLHQVDIAKITTQYLHLINTNLNKQAIEPITDYLVITSRIVEQKANNLLQINDIALDSDFLDNKLRDKLVAQLVEYKRYRESLDDFEKLRINRLAYFSKDNDFNRFIQTVDKSNTEPVKIEDELPNYVSVLKLHHAMNKLMQRWRAQFLANKNISIQELSIEQVQAEILATIKQFGYHSVSLKRVLLKVNHHISLMYFITAFVALLVLINNQIIDIEQTSFDDELYICLLDSSRIEQLQETPEAMVERAVKQRQEAQELARQVAREKAIANAQKREAYLKAKYGKDYLTREQFLKLSPEERAAHVAKMKQLKLVKNDNGRDN" }, "paralog": false, "identity": 0.9881889763779528, "score": 485.0, "evalue": 1.42e-175, "description": "Internal stop codon at 352834. Nonsense mutation around 352834.", "psc": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "gene": "scpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_UPI000AF5CFDF", "kegg_orthology_id": "K05896", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF5CFDF", "UniRef:UniRef50_UPI000AF5CFDF", "KEGG:K05896" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF5CFDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "product": "segregation/condensation protein A" } }, "id": "CNOCLNGIAL_664", "locus": "GCA000143945_00629" }, { "type": "cds", "sequence": "contig_1", "start": 352909, "stop": 353373, "strand": "+", "frame": 1, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "KEGG:K05896", "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF", "UniRef:UniRef90_UPI000AF5CFDF" ], "nt": "TTGGCTACAATTAAGCAATTTGGCTATCATAGTGTTTCGTTAAAACGGGTACTGTTAAAGGTCAACCACCACATTTCCTTAATGTATTTCATTACCGCTTTTGTCGCTTTATTGGTGTTAATTAACAACCAGATTATCGACATTGAGCAGACTAGCTTTGATGATGAGCTTTACATCTGCTTGTTAGACAGTTCGCGGATTAAACAGCTCCAAGAAACGCCAGAAGCCATGGTGGAACGTGCTGTGAAACAACGCCAGGAAGCACAGGAATTAGCCCGTCAAGTAGCTAGAGAAAAAGCAATTGCCAACGCGCAGAAACGCGAAGCTTACCTAAAGGCAAAATATGGCAAGGATTACTTAACACGTGAACAGTTTTTAAAACTGTCTCCCGAAGAACGTGCTGCCCATGTGGCTAAAATGAAACAATTAAAACTAGTAAAGAACGACAATGGACGCGACAATTAA", "aa": "MATIKQFGYHSVSLKRVLLKVNHHISLMYFITAFVALLVLINNQIIDIEQTSFDDELYICLLDSSRIKQLQETPEAMVERAVKQRQEAQELARQVAREKAIANAQKREAYLKAKYGKDYLTREQFLKLSPEERAAHVAKMKQLKLVKNDNGRDN", "aa_hexdigest": "dd3b258a3c792f465deae3bef8eca132", "start_type": "TTG", "rbs_motif": "AACAA", "genes": [ "scpA" ], "truncated": "5-prime", "pseudogene": { "start": 352609, "stop": 353373, "observations": { "mutation": [ 352834 ], "stop": [ 352834 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "query_cov": 1.0, "subject_cov": 0.463855421686747, "identity": 0.987, "score": 274.0, "evalue": 4.15e-90, "gene_start": 1, "gene_end": 154, "reference_start": 179, "reference_end": 332, "reference_sequence": "MLELLIDKKFNLHQVDIAKITTQYLHLINTNLNKQAIEPITDYLVITSRIVEQKANNLLQINDIALDSDFLDNKLRDKLVAQLVEYKRYRESLDDFEKLRINRLAYFSKDNDFNRFIQTVDKSNTEPVKIEDELPNYVSVLKLHHAMNKLMQRWRAQFLANKNISIQELSIEQVQAEILATIKQFGYHSVSLKRVLLKVNHHISLMYFITAFVALLVLINNQIIDIEQTSFDDELYICLLDSSRIEQLQETPEAMVERAVKQRQEAQELARQVAREKAIANAQKREAYLKAKYGKDYLTREQFLKLSPEERAAHVAKMKQLKLVKNDNGRDN" }, "paralog": false, "identity": 0.9921259842519685, "score": 485.0, "evalue": 1e-175, "description": "Internal stop codon at 352834. Nonsense mutation around 352834.", "psc": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "gene": "scpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_UPI000AF5CFDF", "kegg_orthology_id": "K05896", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF5CFDF", "UniRef:UniRef50_UPI000AF5CFDF", "KEGG:K05896" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF5CFDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "product": "segregation/condensation protein A" } }, "id": "CNOCLNGIAL_665", "locus": "GCA000143945_00630" }, { "type": "cds", "sequence": "contig_1", "start": 353357, "stop": 353983, "strand": "+", "frame": 2, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304", "KEGG:K06024", "RefSeq:WP_010874658.1", "SO:0001217", "UniParc:UPI0000139488", "UniRef:UniRef100_P75477", "UniRef:UniRef50_P75477", "UniRef:UniRef90_P75477" ], "nt": "ATGGACGCGACAATTAAGGTAACTAAACCCGTTTTAAAACAAAAGGATAGCAGCGCTGCTAATTTAGTTGCTGCCATTTATGGGTTGTTGTTTGTAAGTGGCGAAAAGGGCTTAACGTTAGCGGAATTAAACCGCGTATTGCGCAAGGTGGGTTTGGAAAAGATTAAGGCCGCTTTAGTGCAGTTAGAGCGCAAGTTAAGTTTAGATGATGAAAGCGGGATTGAAATTAAAAAGTTTGGTCACAGCTTTCGCTTGGTCACCAAAATGGAGATCAAGGACTTTATCCACCGTTACCTACCCAACAAGATTAAAAATCCGCTCAATTCCAAAACAATGGAAGTGTTAGCAATTATTGCTTACAACCAACCCTGCACCCGTCCGCGCATTAATGAAATTAGGGGTGCAGATTCCTTTCAGATTGTCGATGACTTACTAGAAAAGGAGTTAATTGTTGAATTGGGCCGCAAAGACACACCTGGTCGTCCCTTTATTTATGAAGTGTCGCCTAAGTTCTATGATCTGTTTGGGATTAACAGCTTGGATGAACTGCCTAAAGTCGAGAATTTTGATCTCGACAAATTCCGCCAAGGTAGCTTTTTTGACTCTAACCGTTACGGGGATGATTAA", "aa": "MDATIKVTKPVLKQKDSSAANLVAAIYGLLFVSGEKGLTLAELNRVLRKVGLEKIKAALVQLERKLSLDDESGIEIKKFGHSFRLVTKMEIKDFIHRYLPNKIKNPLNSKTMEVLAIIAYNQPCTRPRINEIRGADSFQIVDDLLEKELIVELGRKDTPGRPFIYEVSPKFYDLFGINSLDELPKVENFDLDKFRQGSFFDSNRYGDD", "aa_hexdigest": "79f84eaf58ca309c2c01271745e51478", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139488", "ncbi_nrp_id": "WP_010874658.1", "uniref100_id": "UniRef100_P75477", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139488", "RefSeq:WP_010874658.1", "UniRef:UniRef100_P75477" ] }, "ips": { "uniref100_id": "UniRef100_P75477", "uniref90_id": "UniRef90_P75477" }, "psc": { "uniref90_id": "UniRef90_P75477", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_P75477", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304" ] }, "pscc": { "uniref50_id": "UniRef50_P75477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75477" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "CNOCLNGIAL_666", "locus": "GCA000143945_00631" }, { "type": "cds", "sequence": "contig_1", "start": 354003, "stop": 354989, "strand": "+", "frame": 3, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005829", "GO:0005945", "GO:0006002", "GO:0016208", "GO:0030388", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0070095", "RefSeq:WP_010874659.1", "SO:0001217", "UniParc:UPI000012DB5C", "UniRef:UniRef100_P75476", "UniRef:UniRef50_P75476", "UniRef:UniRef90_P75476" ], "nt": "ATGAGTCCAAAAACAACCAAAAAAATTGCCATTTTAACCTCTGGTGGTGATGCTCCAGGGATGAATGCCACACTAGTTTACCTAACGCGTTATGCCACTAGTTCTGAAATTGAAGTTTTCTTTGTGAAAAACGGTTATTATGGGCTGTACCATGATGAGTTAGTACCCGCTCATCAATTAGATTTGTCAAACAGCTTGTTTAGTGCTGGCACCGTGATTGGTAGTAAGCGCTTTGTCGAATTTAAGGAGTTAAAGGTCCGCGAGCAAGCAGCACAGAATCTTAAAAAGCGCCAAATCGATTACCTAGTGGTTATTGGTGGTGATGGTAGTTACATGGGCGCTAAATTGCTAAGCGAGTTGGGCGTTAACTGTTACTGTTTGCCGGGTACGATTGACAACGACATAAATTCGAGTGAGTTTACAATTGGTTTTCTCACGGCTTTGGAGTCGATTAAGGTCAATGTCCAGGCCGTGTACCACACTACGAAATCACATGAACGGGTCGCCATTGTGGAGGTGATGGGTCGCCATTGTGGTGATTTAGCCATTTTTGGTGCCTTAGCCACCAATGCTGACTTTGTGGTGACTCCTAGCAATAAAATGGATTTAAAGCAGCTAGAAAGTGCTGTCAAAAAGATCTTGCAACACCAAAACCACTGTGTGGTGATTGTTAGTGAAAACATCTATGGCTTTGATGGTTATCCCTCACTTACAGCGATCAAACAGCACTTTGATGCTAACAACATGAAGTGTAACCTGGTGTCCTTGGGTCACACGCAACGCGGATTTGCCCCTACCAGCCTCGAGTTAGTCCAAATTAGTTTAATGGCGCAACACACGATTAACTTAATTGGACAAAACAAGGTTAACCAGGTCATTGGTAATAAGGCCAATGTGCCCGTTAACTATGACTTTGATCAAGCCTTTAACATGCCACCGGTTGATCGCAGCGCTTTAATTGCAGTCATTAATAAAAATATTATTTAA", "aa": "MSPKTTKKIAILTSGGDAPGMNATLVYLTRYATSSEIEVFFVKNGYYGLYHDELVPAHQLDLSNSLFSAGTVIGSKRFVEFKELKVREQAAQNLKKRQIDYLVVIGGDGSYMGAKLLSELGVNCYCLPGTIDNDINSSEFTIGFLTALESIKVNVQAVYHTTKSHERVAIVEVMGRHCGDLAIFGALATNADFVVTPSNKMDLKQLESAVKKILQHQNHCVVIVSENIYGFDGYPSLTAIKQHFDANNMKCNLVSLGHTQRGFAPTSLELVQISLMAQHTINLIGQNKVNQVIGNKANVPVNYDFDQAFNMPPVDRSALIAVINKNII", "aa_hexdigest": "626b61bc27bf7ea64beb6d495aa274b8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012DB5C", "ncbi_nrp_id": "WP_010874659.1", "uniref100_id": "UniRef100_P75476", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DB5C", "RefSeq:WP_010874659.1", "UniRef:UniRef100_P75476" ] }, "ips": { "uniref100_id": "UniRef100_P75476", "uniref90_id": "UniRef90_P75476" }, "psc": { "uniref90_id": "UniRef90_P75476", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_P75476", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005945", "GO:0005829", "GO:0003872", "GO:0016208", "GO:0005524", "GO:0070095", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0030388", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_P75476", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75476" ], "product": "Probable ATP-dependent 6-phosphofructokinase" }, "genes": [ "pfkA" ], "id": "CNOCLNGIAL_667", "locus": "GCA000143945_00632" }, { "type": "cds", "sequence": "contig_1", "start": 355073, "stop": 355858, "strand": "+", "frame": 2, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0006096", "GO:0016020", "GO:0016301", "GO:0030955", "GO:0031639", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_P78031", "UniRef:UniRef90_P78031" ], "nt": "ATGTTAGATGACCCAGCTTACGCTGCGATGAAAGCAGAAGCTTACGCCAACATTGAAAATATTATTAAAAACGGCGTAACGGTAATCCGCTTAAACTTCTCCCATGGTAACCATGAAGAGCAGGCTGTAAGGATTAAGATTGTTCGTGATGTGGCTAAAAAGCTCAACCTCCCGGTGTCAATTATGTTAGACACCAACGGTCCGGAAATCCGCGTCTTTGAAACGGCCCCTGAAGGGTTAAAGATCCTCAAGGACAGTGAGGTGGTTATTAACACCACCACTAAGGAAGTGGCTAAAAATAACCAGTTTAGTGTGAGTGATGCTAGTGGCACTTACAACATGGTCAACGATGTTAAGGTGGGACAAAAAATCCTCGTTGATGATGGTAAGTTAAGCTTAGTCGTAAAGCGGATTGATACGAAGAACAACCAGGTTATTTGTGTTGCCCAAAATGACCACACTATCTTTACCAAAAAGCGCTTAAACCTCCCCAATGCCGACTACTCAATTCCCTTTTTAAGTGCTAAAGACCTACGCGACATTGACTTTGGTTTAACCCACCAAATTGACTACATTGCCGCTTCCTTTGTCAATACAACGGAAAACATCAAGCAACTGCGTGACTACCTCGCTTCTAAGAATGCCAAGCATGTCAAGCTTATTGCCAAAATTGAGTCCAACCACGCTTTAAACAACATTGATGGGATTATTAAAGCGAGTGATGGCATTATGGTAGCACGGGGTGATTTGGGCTTGGAGATCCCATATTACAAGGTGCCTTACTGA", "aa": "MLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSHGNHEEQAVRIKIVRDVAKKLNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTKEVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLVVKRIDTKNNQVICVAQNDHTIFTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKNAKHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPY", "aa_hexdigest": "6cb764dfa725510c574b570bf5492780", "start_type": "ATG", "rbs_motif": null, "genes": [ "pyk" ], "truncated": "both", "pseudogene": { "start": 354995, "stop": 356161, "observations": { "mutation": [ 355061, 355856 ], "stop": [ 355061, 355856 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78031", "query_cov": 1.0, "subject_cov": 0.5137795275590551, "identity": 1.0, "score": 509.0, "evalue": 1.28e-178, "gene_start": 1, "gene_end": 261, "reference_start": 27, "reference_end": 287, "reference_sequence": "MIHHLKRTKIIATCGPALTKKLWTLAMLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSHGNHEEQAVRIKIVRDVAKKLNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTKEVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLVVKRIDTKNNQVICVAQNDHTIFTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKNAKHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPYWQRYMIKACRFFNKRVITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDKQSETFFDYQYNLNYYMANSKARHSEFWKQVVLPLAQKTAPKRKLINSDFKYDFVVHATNNLNEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANVAIKHYQQHGEISQLKQCLGVFHNKIISL" }, "paralog": false, "identity": 0.9948453608247423, "score": 758.0, "evalue": 7.37e-279, "description": "Internal stop codon at 355061, 355856. Nonsense mutation around 355061, 355856.", "psc": { "uniref90_id": "UniRef90_P78031", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_P78031", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0005524", "GO:0006096", "GO:0016020", "GO:0016301", "GO:0030955", "GO:0031639" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78031", "EC:2.7.1.40", "UniRef:UniRef50_P78031", "COG:COG0469", "COG:G", "KEGG:K00873", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0006096", "GO:0016020", "GO:0016301", "GO:0030955", "GO:0031639" ] }, "pscc": { "uniref50_id": "UniRef50_P78031", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78031" ], "product": "Pyruvate kinase" } }, "id": "CNOCLNGIAL_668", "locus": "GCA000143945_00633" }, { "type": "cds", "sequence": "contig_1", "start": 355868, "stop": 356161, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "ATGATAAAAGCGTGTCGTTTCTTTAACAAGCGTGTTATTACCGCAACTCAAATGTTGGACTCTTTGGAAAAAAACATCCAACCAACTCGCGCTGAAGTTACTGATGTGTACTTTGCCGTGGACCGCGGCAATGATGCCACCATGTTAAGTGGAGAAACCGCAAATGGGGCGTTTCCCCTTAATGCTGTGTATGTAATGAAGATGATTGATAAACAGTCCGAAACGTTCTTTGATTACCAGTACAACTTGAACTATTACATGGCCAACTCCAAGGCCCGTCACAGTGAGTTTTGA", "aa": "MIKACRFFNKRVITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDKQSETFFDYQYNLNYYMANSKARHSEF", "aa_hexdigest": "7c59f3e088c1d3bc519e2f601048efa3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 63, "aa_cov": 0.6494845360824743, "hmm_cov": 1.0, "evalue": 1.59502243120298e-24, "score": 82.03192901611328, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 11198.605099999997, "isoelectric_point": 6.551739692687988 }, "id": "CNOCLNGIAL_669", "locus": "GCA000143945_00634" }, { "type": "cds", "sequence": "contig_1", "start": 356168, "stop": 356521, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTGTTACCGTTAGCACAAAAAACAGCACCAAAACGCAAACTGATTAACAGTGACTTTAAATACGACTTTGTGGTGCACGCCACCAACAACCTTAATGAAATCTATGCCTTGTCCAACGCTCGCTTAGCGGCTGCGGTAATTATCTTGACCAATGACCCCCAAGTATACACCGGGCACGGCGTTGATTATGGCATCTTCCCTTACTTAATTGACCAAAAGCCGCAAAGCTTAAGTAAAGCTGAGTTTAAGAGCTTAGCTAATGTTGCCATTAAACACTACCAGCAGCATGGGGAAATTAGTCAATTAAAGCAGTGTTTGGGAGTATTTCACAATAAGATCATTAGCTTATAA", "aa": "MVLPLAQKTAPKRKLINSDFKYDFVVHATNNLNEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANVAIKHYQQHGEISQLKQCLGVFHNKIISL", "aa_hexdigest": "e13dcbdd189b0752fd87ad3cd15a034b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13110.00559999999, "isoelectric_point": 9.240269660949707 }, "id": "CNOCLNGIAL_670", "locus": "GCA000143945_00635" }, { "type": "tRNA", "sequence": "contig_1", "start": 356561, "stop": 356637, "strand": "+", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 60.0, "nt": "GGTGCCTTAGCCAAGTGGATTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 356596, 356598 ], "id": "CNOCLNGIAL_1", "locus": "GCA000143945_00636" }, { "type": "tRNA", "sequence": "contig_1", "start": 356689, "stop": 356765, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 83.1, "nt": "CGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 356706, 356708 ], "id": "CNOCLNGIAL_2", "locus": "GCA000143945_00637" }, { "type": "tRNA", "sequence": "contig_1", "start": 356770, "stop": 356846, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 82.2, "nt": "GGCTGGATACCTCAGTTGGTTAGAGGACCCGGTTCATACCCGGGTTGTCGTGAGTTCGAATCTCACTCCAGCCACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 356804, 356806 ], "id": "CNOCLNGIAL_3", "locus": "GCA000143945_00638" }, { "type": "tRNA", "sequence": "contig_1", "start": 356857, "stop": 356932, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 74.7, "nt": "GGATCTATAGCTCAATGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCGAGTCCTGTTAGATCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "CNOCLNGIAL_4", "locus": "GCA000143945_00639" }, { "type": "tRNA", "sequence": "contig_1", "start": 356973, "stop": 357063, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 78.9, "nt": "GGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTGAAAAGCACGCGAGGGTTCGAATCCCTCAGTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 356992, 356994 ], "id": "CNOCLNGIAL_5", "locus": "GCA000143945_00640" }, { "type": "tRNA", "sequence": "contig_1", "start": 357076, "stop": 357152, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 77.7, "nt": "CGCGGGATAGAGCAGTCGGTTAGCTCGTCAGGCTCATAATCTGAAGGTCGGGGGTTCGAATCCCTCTCCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "CNOCLNGIAL_6", "locus": "GCA000143945_00641" }, { "type": "tRNA", "sequence": "contig_1", "start": 357154, "stop": 357227, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 55.6, "nt": "GGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 357186, 357188 ], "id": "CNOCLNGIAL_7", "locus": "GCA000143945_00642" }, { "type": "tRNA", "sequence": "contig_1", "start": 357229, "stop": 357304, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 77.8, "nt": "GGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 357262, 357264 ], "id": "CNOCLNGIAL_8", "locus": "GCA000143945_00643" }, { "type": "cds", "sequence": "contig_1", "start": 357500, "stop": 357775, "strand": "+", "frame": 2, "gene": null, "product": "arginine deiminase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132F68E0", "UniRef:UniRef90_UPI00132F68E0" ], "nt": "ATGAAGTACAACATCAACGTTCATTCCGAAATTGGTCAACTGCAAACCGTTTTAGTCCACACCCCTGGTAATGAAATTAGACGGATTTCACCAAGACGGTTGGATGATTTACTGTTTTCAGCAGTAATAGAACCAGATACTGCCATCCAGGAACACCAAACCTTTTGTCAGTTACTGCAAGAACAAAACATTGAAGTGGTGCAACTGACTGATTTAACAGCCACCACCTTTGACAAGGCAAATGCTACAGCGCAAAACCAGTTTATTGAAACTTGA", "aa": "MKYNINVHSEIGQLQTVLVHTPGNEIRRISPRRLDDLLFSAVIEPDTAIQEHQTFCQLLQEQNIEVVQLTDLTATTFDKANATAQNQFIET", "aa_hexdigest": "e88084dfe637df752a322788b98e60e0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 357500, "stop": 358054, "observations": { "mutation": [ 357773 ], "stop": [ 357773 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00132F68E0", "query_cov": 1.0, "subject_cov": 0.4945652173913043, "identity": 1.0, "score": 179.0, "evalue": 9.54e-56, "gene_start": 1, "gene_end": 91, "reference_start": 1, "reference_end": 91, "reference_sequence": "MKYNINVHSEIGQLQTVLVHTPGNEIRRISPRRLDDLLFSAVIEPDTAIQEHQTFCQLLQEQNIEVVQLTDLTATTFDKANATAQNQFIETWLDQAEPKLTPEHRKVAKQYLLEQKAKSTLSMVRSMMGGIDKRKVAAANTINGDFLVDPMPNLYFTRDPFASIGHGISINRMKYLTRRRQTLF" }, "paralog": false, "identity": 0.9891304347826086, "score": 360.0, "evalue": 1.28e-129, "description": "Internal stop codon at 357773. Nonsense mutation around 357773.", "psc": { "uniref90_id": "UniRef90_UPI00132F68E0", "product": "arginine deiminase family protein", "uniref50_id": "UniRef50_UPI00132F68E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00132F68E0", "UniRef:UniRef50_UPI00132F68E0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00132F68E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132F68E0" ], "product": "arginine deiminase family protein" } }, "id": "CNOCLNGIAL_671", "locus": "GCA000143945_00644" }, { "type": "cds", "sequence": "contig_1", "start": 357866, "stop": 358180, "strand": "+", "frame": 2, "gene": null, "product": "Arginine deiminase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCR3", "UniRef:UniRef90_A0AAN5BCR3" ], "nt": "ATGGTACGCTCGATGATGGGTGGGATTGATAAGCGTAAGGTAGCTGCTGCTAACACCATTAACGGTGATTTTTTAGTAGATCCGATGCCTAACCTTTACTTTACCCGCGATCCGTTTGCCTCGATTGGCCATGGTATTAGTATTAACCGGATGAAGTATTTAACGCGGCGGCGTGAAACCTTATTTGCTAGTTTTATTTTGCCAACCACCCCGATTATTGCCGCACGCAAATTTACTTTAAACCAATTGACATGGGCACAATTGAGGGTGGTGATATTTTTGTATATGACCAACAAACAGTGGTGGTGGGGTTGA", "aa": "MVRSMMGGIDKRKVAAANTINGDFLVDPMPNLYFTRDPFASIGHGISINRMKYLTRRRETLFASFILPTTPIIAARKFTLNQLTWAQLRVVIFLYMTNKQWWWG", "aa_hexdigest": "d91aeb3693cb177712dd3533e303bb58", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 357566, "stop": 358216, "observations": { "mutation": [ 357773, 358178 ], "stop": [ 357773, 358178 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN5BCR3", "query_cov": 1.0, "subject_cov": 0.4369747899159664, "identity": 1.0, "score": 215.0, "evalue": 3.24e-69, "gene_start": 1, "gene_end": 104, "reference_start": 123, "reference_end": 226, "reference_sequence": "MKYNINVHSEIGQLQTVLVHTPGNEIRRISPRRLDDLLFSAVIEPDTAIQEHQTFCQLLQEQNIEVVQLTDLTATTFDKANATAQNQFIETWLDQAEPKLTPEHRKVAKQYLLEQKAKSTLSMVRSMMGGIDKRKVAAANTINGDFLVDPMPNLYFTRDPFASIGHGISINRMKYLTRRRETLFASFILPTTPIIAARKFTLNQLTWAQLRVVIFLYMTNKQWWWGWVNGQPKLPLMF" }, "paralog": false, "identity": 0.9907407407407407, "score": 427.0, "evalue": 6.68e-155, "description": "Internal stop codon at 357773, 358178. Nonsense mutation around 357773, 358178.", "psc": { "uniref90_id": "UniRef90_A0AAN5BCR3", "product": "Arginine deiminase domain-containing protein", "uniref50_id": "UniRef50_A0AAN5BCR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN5BCR3", "UniRef:UniRef50_A0AAN5BCR3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAN5BCR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCR3" ], "product": "Arginine deiminase domain-containing protein" } }, "id": "CNOCLNGIAL_672", "locus": "GCA000143945_00645" }, { "type": "cds", "sequence": "contig_1", "start": 358165, "stop": 358410, "strand": "+", "frame": 1, "gene": null, "product": "Arginine deiminase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742", "UniRef:UniRef90_A0AB38W742" ], "nt": "GTGGTGGTGGGGTTGAGTGAACGGACAACCGAAGCTGCCATTAATGTTTTAGCGAAAAAGATTCAACAAGACAGTAGCACTTCCTTTAAGCGGATCTTTGTCATTAACGTTCCACAACTACCGAACTTAATGCATTTAGACACGTGGCTGACAATGTTAGACCGCAACAAGTTCTTGTACTCCCCGAACATGTTGGCGGTTTTAAAGGCGTGGCGGATTGACTTGACTGATCCGGCTTTAAAGTGA", "aa": "MVVGLSERTTEAAINVLAKKIQQDSSTSFKRIFVINVPQLPNLMHLDTWLTMLDRNKFLYSPNMLAVLKAWRIDLTDPALK", "aa_hexdigest": "05457bc82bc16dba783c736c0e48e5fb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 358117, "stop": 358713, "observations": { "mutation": [ 358408 ], "stop": [ 358408 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W742", "query_cov": 1.0, "subject_cov": 0.4090909090909091, "identity": 0.988, "score": 160.0, "evalue": 1.91e-48, "gene_start": 1, "gene_end": 81, "reference_start": 17, "reference_end": 97, "reference_sequence": "MGTIEGGDIFVYDQQTVVVGLSERTTEAAINVLAKKIQQDSSTSFKRIFVINVPQLPNLMHLDTWLTMLDRNKFLYSPNMLAVLKAWRIDLTDPALKWNEIAGDLSTILHTIIGQKPMLIPIAGADANQTEIDIETHFDGTNYLTIAPSVVVGYARNKLTHQALEAAGVKVIAFKGNQLSLGMGSARCMSMPLVRKPL" }, "paralog": false, "identity": 0.9949494949494949, "score": 387.0, "evalue": 2.22e-140, "description": "Internal stop codon at 358408. Nonsense mutation around 358408.", "psc": { "uniref90_id": "UniRef90_A0AB38W742", "product": "Arginine deiminase", "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W742", "UniRef:UniRef50_A0AB38W742" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742" ], "product": "Arginine deiminase" } }, "id": "CNOCLNGIAL_673", "locus": "GCA000143945_00646" }, { "type": "cds", "sequence": "contig_1", "start": 358468, "stop": 358713, "strand": "+", "frame": 1, "gene": null, "product": "Arginine deiminase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742", "UniRef:UniRef90_A0AB38W742" ], "nt": "ATGCTCATTCCCATTGCCGGAGCGGATGCTAACCAAACCGAAATTGACATTGAAACCCACTTTGATGGCACCAACTACCTCACCATCGCTCCATCGGTAGTAGTGGGGTATGCGCGCAATAAATTAACACACCAGGCCTTGGAAGCGGCTGGGGTTAAAGTAATTGCCTTTAAGGGGAACCAGCTGTCTTTGGGAATGGGTTCTGCACGTTGTATGTCGATGCCATTAGTACGCAAACCACTTTAA", "aa": "MLIPIAGADANQTEIDIETHFDGTNYLTIAPSVVVGYARNKLTHQALEAAGVKVIAFKGNQLSLGMGSARCMSMPLVRKPL", "aa_hexdigest": "fdbd022901da6bce238cd7922f0467da", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 358168, "stop": 358713, "observations": { "mutation": [ 358408 ], "stop": [ 358408 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W742", "query_cov": 1.0, "subject_cov": 0.4090909090909091, "identity": 1.0, "score": 161.0, "evalue": 9.48e-49, "gene_start": 1, "gene_end": 81, "reference_start": 118, "reference_end": 198, "reference_sequence": "MGTIEGGDIFVYDQQTVVVGLSERTTEAAINVLAKKIQQDSSTSFKRIFVINVPQLPNLMHLDTWLTMLDRNKFLYSPNMLAVLKAWRIDLTDPALKWNEIAGDLSTILHTIIGQKPMLIPIAGADANQTEIDIETHFDGTNYLTIAPSVVVGYARNKLTHQALEAAGVKVIAFKGNQLSLGMGSARCMSMPLVRKPL" }, "paralog": false, "identity": 0.994475138121547, "score": 353.0, "evalue": 7.91e-127, "description": "Internal stop codon at 358408. Nonsense mutation around 358408.", "psc": { "uniref90_id": "UniRef90_A0AB38W742", "product": "Arginine deiminase", "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W742", "UniRef:UniRef50_A0AB38W742" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742" ], "product": "Arginine deiminase" } }, "id": "CNOCLNGIAL_674", "locus": "GCA000143945_00647" }, { "type": "cds", "sequence": "contig_1", "start": 358741, "stop": 358938, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATTAACTTAAAAGGTCGTAGCCTTGACTCTGCTTTAAACTTTACTACTGCTCAAATTAACTATTTAATTGACCTCGCCATTGATTTAAAACGCAGTAAATACCAAGTTGCACATCCAGAACCGTCCCTTAGTGGGGAAGAACATTGTTTTACTGTTCCAAAAAGATTCTACCCGTACCCGTTGTGCCTTTGA", "aa": "MPINLKGRSLDSALNFTTAQINYLIDLAIDLKRSKYQVAHPEPSLSGEEHCFTVPKRFYPYPLCL", "aa_hexdigest": "dc06425fa9cd9654cf3f7b8eb3c9d9a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7425.520999999999, "isoelectric_point": 7.799336814880372 }, "id": "CNOCLNGIAL_675", "locus": "GCA000143945_00648" }, { "type": "cds", "sequence": "contig_1", "start": 358877, "stop": 359125, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "GTGGGGAAGAACATTGTTTTACTGTTCCAAAAAGATTCTACCCGTACCCGTTGTGCCTTTGAAGTGGCTGCCTTTGACTTGGGTATGGGTTGCACTTACATCGGCCCTTCTGGTTCCAATTTTGGTAAGAAGGAATCGATTGAAGACACTGCTAAAGTGTTGGGTTCAATGTATGACGGAATTGAATTCCGTGGCTTTAAGCAAAGCGATGTTGATGCTTTGGTAAAGTACTCTGGTGTTCCTGTTTGA", "aa": "MGKNIVLLFQKDSTRTRCAFEVAAFDLGMGCTYIGPSGSNFGKKESIEDTAKVLGSMYDGIEFRGFKQSDVDALVKYSGVPV", "aa_hexdigest": "7fd32847496358fd8ec335c9af44c23c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 87, "aa_cov": 0.9878048780487805, "hmm_cov": 1.0, "evalue": 1.158063379520271e-24, "score": 82.61307525634766, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 8894.083699999996, "isoelectric_point": 6.150058555603027 }, "id": "CNOCLNGIAL_676", "locus": "GCA000143945_00649" }, { "type": "cds", "sequence": "contig_1", "start": 359132, "stop": 359464, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "TTGACTGATGCGGAACACCCTACCCAAATGCTAGCGGATTACATGACGATTAAGGAGTTGAAGGGTGATTTGAAGGGGCGCAAAATTGTCTTTGCTGGTGATATTAAAAATAATGTGGCACGCTCGTTGATGATTGGGGCAGCTTTTGTTGGTATGGACATAGTGCTAGTAGGTCCTAAAGCACAGCATGAATTAGTTCAAAATGGTGCTGGGTATAAAGAGGTCTTTGAGCAGTGTCAAGCATTGTTTCAACTCAATGGTGGTAGTGTTAGCTTTAGCACTGATAAGCTGCAAGCGGCTAAGAATGCTGATGTAATTTACACCGATGTGTGA", "aa": "MTDAEHPTQMLADYMTIKELKGDLKGRKIVFAGDIKNNVARSLMIGAAFVGMDIVLVGPKAQHELVQNGAGYKEVFEQCQALFQLNGGSVSFSTDKLQAAKNADVIYTDV", "aa_hexdigest": "334c7426cbdec7e6abf218bd0e67c9d4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 86, "aa_cov": 0.7818181818181819, "hmm_cov": 0.9186046511627907, "evalue": 2.5194138672812167e-19, "score": 65.3192138671875, "start": 25, "stop": 110 } ], "seq_stats": { "molecular_weight": 11936.594999999994, "isoelectric_point": 5.569902992248534 }, "id": "CNOCLNGIAL_677", "locus": "GCA000143945_00650" }, { "type": "cds", "sequence": "contig_1", "start": 359471, "stop": 359782, "strand": "+", "frame": 2, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "TTGGGTGAAGACTTTAGCTTATTTGAAGAACGGATTCAGGAGCTGGGTCAATTTCAAGTTGACATGGCTATGATCAAAGCAGCTAAGAGTGATGTGATCTTCTTGCACTGTCTCCCCGCTTTCCATGATGATCACACTAGCTTTTCACTGGAAATTAAACAAAAGTTAGGTAAAAAATATCCGGTGGTGAAAACGGGTGCGATGGAAGTGACTGATACGGTGTTTCAGTCCAAGCATAACATGGCCTTTATCCAAGCAGAAAACCGCTTGCACACGATTAAGGCCGTTATTTTAGCTACTTTAGGGTATTAA", "aa": "MGEDFSLFEERIQELGQFQVDMAMIKAAKSDVIFLHCLPAFHDDHTSFSLEIKQKLGKKYPVVKTGAMEVTDTVFQSKHNMAFIQAENRLHTIKAVILATLGY", "aa_hexdigest": "f2e0a1078b4b5f84a9b7db0a05cf2ea8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.8349514563106796, "subject_cov": 0.9885057471264368, "identity": 0.5, "score": 79.0, "evalue": 2.91e-17, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "CNOCLNGIAL_678", "locus": "GCA000143945_00651" }, { "type": "cds", "sequence": "contig_1", "start": 359785, "stop": 360714, "strand": "+", "frame": 1, "gene": "arcC", "product": "Carbamate kinase-like protein", "db_xrefs": [ "COG:COG0549", "COG:E", "GO:0005829", "GO:0008804", "GO:0019546", "RefSeq:WP_010874663.1", "SO:0001217", "UniParc:UPI0000125DAF", "UniRef:UniRef100_P78030", "UniRef:UniRef50_P78030", "UniRef:UniRef90_P78030" ], "nt": "ATGCAAAAAATTGTCATAGCATTGGGTGGTAATGCATTAGGTAACAATCCCACCGAACAAAAGGACTTGGTACAACTACCAGCACGACAGGCGGTTGCTTTGTTAAAGCAGGGTTATCAGATATTATTGGGTCATGGTAACGGTCCACAGGTTGGCATGATCTACAACGCCTTTAGTGCGGCCAAACAAGTCAACCCTAATACACCCACTGTACCATTTGCTGAAAGTGGGGCAATGTCTCAGGGTTACATTGGACTGCATTTGTTAACAGCATTGTACAATGAGCTGCTACACCAAAAACTACCCCATCAAGCCGTGTACTTTTTAACACAAACCTTAGTAGAAGCGAGCGATCCTGCTTTTCAAAACCCAAATAAACCAGTTGGTCCGTTTTACAACACAGAGGAAACAGCACGCTCAGCTAATCCAAACAGCACCGTGGTGGAAGATGCAGGGCGGGGTTGGCGTAAAGTAGTAGCGTCCCCCAAACCAGTTGATGTTTTGGGAATTGATGCCATTAAAAGTAGCTTTAACCAAGGCAACTTGGTGATTGTTGGTGGCGGTGGCGGTGTACCCACAATTAAGACCAAAAGTGGTTATGCAACGGTTGATGGGGTGATTGATAAGGATCTAGCATTGAGTGAAATTGCCATTAAGGTAGAGGCTGACCTGTTTGTTATTCTCACTGCCGTGGACTTTGTCTACATTAACTATGGTCAACCAAACGAGCAAAAGCTAACCTGCATTAACACCAAGGAAGCTAAGACCTTAATGGCCGCAAACCAGTTTGCTAAAGGTAGTATGCTCCCCAAGGTAGAAGCGTGTTTAAACTTTGTCCAGTCGGGTACCAATAAGACGGCCATTATTGCCCAATTAGACCAATTAGCTGCGGCCATTGCGGGCAAGATTGGTACTAAAATTGTCAAGTAG", "aa": "MQKIVIALGGNALGNNPTEQKDLVQLPARQAVALLKQGYQILLGHGNGPQVGMIYNAFSAAKQVNPNTPTVPFAESGAMSQGYIGLHLLTALYNELLHQKLPHQAVYFLTQTLVEASDPAFQNPNKPVGPFYNTEETARSANPNSTVVEDAGRGWRKVVASPKPVDVLGIDAIKSSFNQGNLVIVGGGGGVPTIKTKSGYATVDGVIDKDLALSEIAIKVEADLFVILTAVDFVYINYGQPNEQKLTCINTKEAKTLMAANQFAKGSMLPKVEACLNFVQSGTNKTAIIAQLDQLAAAIAGKIGTKIVK", "aa_hexdigest": "993d22e63285c239bdd26b61078511c6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125DAF", "ncbi_nrp_id": "WP_010874663.1", "uniref100_id": "UniRef100_P78030", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125DAF", "RefSeq:WP_010874663.1", "UniRef:UniRef100_P78030" ] }, "ips": { "uniref100_id": "UniRef100_P78030", "uniref90_id": "UniRef90_P78030" }, "psc": { "uniref90_id": "UniRef90_P78030", "gene": "ArcC", "product": "Carbamate kinase-like protein", "uniref50_id": "UniRef50_P78030", "cog_id": "COG0549", "cog_category": "E", "go_ids": [ "GO:0005829", "GO:0008804", "GO:0019546" ] }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [ "arcC" ], "id": "CNOCLNGIAL_679", "locus": "GCA000143945_00652" }, { "type": "cds", "sequence": "contig_1", "start": 360717, "stop": 361520, "strand": "+", "frame": 3, "gene": null, "product": "amino acid permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244EE166" ], "nt": "ATGAAACAACAAAAACCCAAAATTAGCTTTATAGCAGCGATGCTAATTGTTATCGGCTCGTCCATAGGTGCTGGTATCTTTTTTAAATCCAGCACTGTTTTGGAAAATTCCCAGGCCTCTTTAGTGCTAGCGATTTTTAACTGGTTGGTAGCTTCAGTGGCAGTGATTGCTATGGCCTTGGCATTAATTGAAATTGCCAGTGTTCGCAACGATAATCTCTCGATTATTAGCTGGGTCAAAGTGTTTAACCGGCGCTGGTTGTACCATGGTTGTAAGAATTTTATGACCTATTTGTACCTACCGTTGACCTTCTTCTTTATGCCGCTGTACTTTATCTGTTCCATTCAAGATGGGTTTCGCGGTTTGTTGGGACTGGAAACCGGAGCGCATTTCAATACTAGTGTGGACTGGTTAATCTGGTTAGCTTTAGCACTTATTATTACCACTTACTTCTTGACAATTCCACCACTGTACGCTAAGGTAGGGAACATCCAAAACATGGTGGTAAGCGCCGTGAAGTTTATTCCTTTAGTGTTTGTGCCTATCATCGGTTTTATTGTAGCGGGTACAGGTAACGGTGAATTAAAAAACGTTAAGGCATTAGTGCAACCCCCACAAATTAATGGTGCGACTGCTTCCTTTACCCAATTAGTACAAGCGGGGTATGGAATTACTCGTTTTACGGGAATTGGTGCTGGGATGGGTTCCTTTATTTCGATTGCCGCCATTTTCTTTGCGTACGATGGTTTTTACGTAACAGCTGGACTGCAGTCAGAGATGCGTGAACCGAAAAAAACACCGTGA", "aa": "MKQQKPKISFIAAMLIVIGSSIGAGIFFKSSTVLENSQASLVLAIFNWLVASVAVIAMALALIEIASVRNDNLSIISWVKVFNRRWLYHGCKNFMTYLYLPLTFFFMPLYFICSIQDGFRGLLGLETGAHFNTSVDWLIWLALALIITTYFLTIPPLYAKVGNIQNMVVSAVKFIPLVFVPIIGFIVAGTGNGELKNVKALVQPPQINGATASFTQLVQAGYGITRFTGIGAGMGSFISIAAIFFAYDGFYVTAGLQSEMREPKKTP", "aa_hexdigest": "ccea19e1dcd65445d1d7b1eb7f7c3e4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00244EE166", "query_cov": 0.9775280898876404, "subject_cov": 0.8642384105960265, "identity": 0.552, "score": 275.0, "evalue": 3.12e-89, "valid": false, "product": "amino acid permease", "uniref50_id": "UniRef50_UPI00244EE166" }, "pscc": { "uniref50_id": "UniRef50_UPI00244EE166", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244EE166" ], "product": "amino acid permease" }, "genes": [], "id": "CNOCLNGIAL_680", "locus": "GCA000143945_00653" }, { "type": "cds", "sequence": "contig_1", "start": 361524, "stop": 361727, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTAGGTTTACTAATTACTACGCTGTTTTACCTTATCTTAGCAGTGGCACTTTCCATTAACGGTGGTTTGTTTAGTGGTATGGAAGAGTCCATGGGAAAGCTGTTTAACAATAAGCGTGCCGGGCAAATTGTCTTTGGTGTTGTTAACCTAATGATTGGCATAGGTGTCTTGGGTATTATTAACGGTTTTGCACTGTGA", "aa": "MFLGLLITTLFYLILAVALSINGGLFSGMEESMGKLFNNKRAGQIVFGVVNLMIGIGVLGIINGFAL", "aa_hexdigest": "33b2f0ba50e7efbad43f86d9b5ef9918", "start_type": "TTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7092.5383999999995, "isoelectric_point": 8.25416126251221 }, "id": "CNOCLNGIAL_681", "locus": "GCA000143945_00654" }, { "type": "cds", "sequence": "contig_1", "start": 361785, "stop": 361994, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGGGCAGATTGAACCCCAACAAGCCAGTGGTGGGGGTAATTTACTGTTTGGTACTTTCTTTGACTGTTCAAGTGCTGTTTACGGTAATAGGTGCTTTGGCTTACCTCCCGACAGTAGCGGATTACAAAAATTACGTTAACACTGAAATTGACAAGCTCAATTCGATGCAATGGTTGTATTCGTTTTCCGATTTAATGGCTACTTGA", "aa": "MQGRLNPNKPVVGVIYCLVLSLTVQVLFTVIGALAYLPTVADYKNYVNTEIDKLNSMQWLYSFSDLMAT", "aa_hexdigest": "e0149582a18217d9572dc2a154a50fd6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7745.0167, "isoelectric_point": 5.916052436828613 }, "id": "CNOCLNGIAL_682", "locus": "GCA000143945_00655" }, { "type": "cds", "sequence": "contig_1", "start": 362121, "stop": 362414, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTAACTGCATTAGTGTTTTTGTAACTATCATTGAGCCCTTCATTAACCTCTTTTTACTGTTTGGTTATGATGAAACAGTGGCACACACAGTGCTCGGTAATGACTTCATTGAATTGAATGAATTAGTGATAGGACGGGTAATGCTGATAGTAGTGTTAGTTTTCTTTGCCATAATTAGCTTTCTCCCTGTTTATGTGGAGGATCAATACCACAAACGTAAGTTTGGTAGTTTAGCTAACTATCAGCAATATGTACAACAACATTTAGCACACTCCACCATTAACGGTTAA", "aa": "MVNCISVFVTIIEPFINLFLLFGYDETVAHTVLGNDFIELNELVIGRVMLIVVLVFFAIISFLPVYVEDQYHKRKFGSLANYQQYVQQHLAHSTING", "aa_hexdigest": "f9b3ac9992a53fa9f0deb599d6c69132", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11138.88329999999, "isoelectric_point": 5.32839412689209 }, "id": "CNOCLNGIAL_683", "locus": "GCA000143945_00656" }, { "type": "cds", "sequence": "contig_1", "start": 362470, "stop": 363060, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCGGTACTAGTAACATAGTAAGGATCGGTGTAAGCGTTTGGGTCCTGATAAGCATTGGGATCTACATAGGCATAGTACGCTTGGTTGGGATCTTGAGCATAGTCTTGTGGATATGCTTGACCATCCTGACCATAGCCGTAATAAGCATTGGGATCGGTGTAGTTATTAGGATCTACATAAGCGTTTGGGTCCTGATAAGCATTGGGATCTACATAGGCGTTAGGATCGGTGTAAGCGTTTGGGTCCTGATAAGCATTGGGATCGGCATAGTACGCTTGGTTGGGATCTTGAGCATAGTCTTGTGGATATGCTTGACCATCCTGACCATAGCCGTAATAAGCCGCTGCATCAGGATAGGTATTCGGATCGGGGTAATACGCTTGGTTGGGATCAACTGCTTGGTCGGGGTCGTAATAAGTACTACCATCACCATAGTACTGCTGCTGATTGGGATCGTATGCAGTAGCTTGTTGGTCAGACTGATTTCAACCTGGTTTATTTATATCCATTTACTGTCTTTAAAAGGTGCCAACAGCAAAGCTGACACTATATCATTAATTTTAATTTATCTAAAAAAATGCAAATGA", "aa": "MIGTSNIVRIGVSVWVLISIGIYIGIVRLVGILSIVLWICLTILTIAVISIGIGVVIRIYISVWVLISIGIYIGVRIGVSVWVLISIGIGIVRLVGILSIVLWICLTILTIAVISRCIRIGIRIGVIRLVGINCLVGVVISTTITIVLLLIGIVCSSLLVRLISTWFIYIHLLSLKGANSKADTISLILIYLKKCK", "aa_hexdigest": "5203b79bca0e66803b71ff4d4405b330", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21194.298400000036, "isoelectric_point": 10.371238517761228 }, "id": "CNOCLNGIAL_684", "locus": "GCA000143945_00657" }, { "type": "cds", "sequence": "contig_1", "start": 363304, "stop": 363501, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCAAAGCGATTGATACTGTCAAGGAAAACAGCGCTAACTTGCAAATTATGAAGCAGAACTTTGAACGCCAACTCAAAGAACAGCAAACCCAACGGCTTACAAGTAATACTGACGCGGAAAAGATTGGTGCTAAGATCAACCAATTAGAGGAACGCATGCAACGTTTATCGCGCACTATGGAAAGTGTGGAGTGA", "aa": "MRKAIDTVKENSANLQIMKQNFERQLKEQQTQRLTSNTDAEKIGAKINQLEERMQRLSRTMESVE", "aa_hexdigest": "039a7f886538e5d6573fdcfaf0014f14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7666.626500000002, "isoelectric_point": 9.297517585754395 }, "id": "CNOCLNGIAL_685", "locus": "GCA000143945_00658" }, { "type": "cds", "sequence": "contig_1", "start": 363592, "stop": 363771, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATGTTTGAAAAGGTATTAATGATGAACTTACTGCGCAGCACCACTCCAGTCCAACCTCCAGTGCAGTACTTTACTCCTCAACCCCTTACAGCATCGCCACGTCCAGTGTATGAAGAACCGATCTCCGCTTCGTTCCGCCGTCGTGGTTACCGTGGTGATGAATTTTACGAATAA", "aa": "MSMFEKVLMMNLLRSTTPVQPPVQYFTPQPLTASPRPVYEEPISASFRRRGYRGDEFYE", "aa_hexdigest": "16f35960ec594689398f294edc8c290c", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6943.915500000001, "isoelectric_point": 8.140503501892091 }, "id": "CNOCLNGIAL_686", "locus": "GCA000143945_00659" }, { "type": "cds", "sequence": "contig_1", "start": 363791, "stop": 364204, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATACTGACAAGAAGTTCCCCTTGCAACCAGTTTATGACACTGGGTTTGATGATGGGTATTTACAGCGTGATTATGAAAAGCGTTTAGAAAGTGCTGCTGCTAATGATGCACAAACAGTGGAACTACAAACCCAGCTGTTAGCAGAGATTAAAAACCTTGAAAACGAAATCAAGGCCCTAAAGGCGCAGGAATCACGCCAACCCGATCCGCACAACAATGCCCGGATTCAAAGCTTAGAAGCTAGCTTAAACCGTTTGGTCAATGAGTACAACAACTTTGAATTCCAAAAGAACTATATGGTTGATCGGGTAGCAGAGTTAAATAACAAAGCGCGTTTTTTTAAGGACGAATTAAAGCGTCTCCAACAAGAAAACGCCGCTTTTGTTAATTCCCGTTATGCCAATTGA", "aa": "MNDTDKKFPLQPVYDTGFDDGYLQRDYEKRLESAAANDAQTVELQTQLLAEIKNLENEIKALKAQESRQPDPHNNARIQSLEASLNRLVNEYNNFEFQKNYMVDRVAELNNKARFFKDELKRLQQENAAFVNSRYAN", "aa_hexdigest": "b4229804fecd50ef046c4e9a4b826547", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16034.536799999983, "isoelectric_point": 5.114622688293457 }, "id": "CNOCLNGIAL_687", "locus": "GCA000143945_00660" }, { "type": "cds", "sequence": "contig_1", "start": 364445, "stop": 364567, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCATCGAGTATGAAGCCACATACCAGGACTTGGTGAGTGCGGATGCGGAGTTGCAAAACGTTTATGAAACAATTGCCCAAAACCAGGCCAACTTCCAAAAGCAGTGTGATGCTTATTGA", "aa": "MGIEYEATYQDLVSADAELQNVYETIAQNQANFQKQCDAY", "aa_hexdigest": "255ce307c715f42848a3b54350a7048e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4576.8905, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_688", "locus": "GCA000143945_00661" }, { "type": "cds", "sequence": "contig_1", "start": 364616, "stop": 365512, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGATGAAGAATCGGCACTTAAGGTGCGTTTAAATGACGCTGATTTTTACATTAACTCACGCTTAGCAGAATTAGATGATTTAACTAGCAAGATTAACGAACGTGATTTTGTTTCTAAAGAACAAGCTCAAGACGTTAAAGCTAGTTTAGCTAACCTCACTAAGGAAAAAGAACGTTTGAGCGCTGAGAAGGATAGCTTTGAAAGGTTAAGGAACACCGCTTTAAACGACATTAACCGAATGGAGCAGGAAAACGCGTTGTTTGCTAAACACTTAGAACAGCAACAATACGAGTTTGAAAGAAAGCAACAGGAAAGCTTATTAAAGCTAGAAACAGAGCATAAACAGCTCCAAAAACGAATTGGTGAGTTCAAAATCGAGAGTGAAGCTAAAAGTGAAGCGTTACTGATACAAGAACGTGAACTGTTGGAAAAACGCCGGGAAATTGATGATTTGTTAACCCAAGCCAGCTTGGAGTATGAACAACAACGCCAGACGAATCAGGTCTTAAAGGAAAAGCACCGTCAAGTCCAACAACACTTCCAAAACCTCGTGCATGCTAAAAAGAAATTGGATCAAAAGCGCCATTATTTAGCGGAACAAAAGCGGATTGATGAAGAACAGATTTTTAAGTTAAAGGAAAAGATTGCCACTGAACGCCGCGAATTGGAGAAGCTTTATCTAGTTAAAAAACAAAAGCAAGACCAAAAGGAAAACGATTTACTGATTTTTGAAAAACAGTTAAGGCAGTACCAAGCTGACTTTGAAAACGAAATAGAAGAGAAGCAAAACGAGCTGTTTGCATCCCAAAAATCACTCCAAAAATCGTTTACACAACTCAAAAATAAGGAAGCGGAATTAAACCAAAAGGCACAAAAGATTGCAGAGGATTGA", "aa": "MVDEESALKVRLNDADFYINSRLAELDDLTSKINERDFVSKEQAQDVKASLANLTKEKERLSAEKDSFERLRNTALNDINRMEQENALFAKHLEQQQYEFERKQQESLLKLETEHKQLQKRIGEFKIESEAKSEALLIQERELLEKRREIDDLLTQASLEYEQQRQTNQVLKEKHRQVQQHFQNLVHAKKKLDQKRHYLAEQKRIDEEQIFKLKEKIATERRELEKLYLVKKQKQDQKENDLLIFEKQLRQYQADFENEIEEKQNELFASQKSLQKSFTQLKNKEAELNQKAQKIAED", "aa_hexdigest": "253137fb854deee897549680b74679a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35790.806600000054, "isoelectric_point": 5.902581596374512 }, "id": "CNOCLNGIAL_689", "locus": "GCA000143945_00662" }, { "type": "cds", "sequence": "contig_1", "start": 365606, "stop": 368674, "strand": "+", "frame": 2, "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0020035", "SO:0001217", "UniRef:UniRef50_P75471", "UniRef:UniRef90_P75471" ], "nt": "TTGGAAGCACGTACGCAGTTTGACAACCGTGTCAGTTTGCTCTCAGCACGCTTTAAACAAAAACAAGCAGAATTAGTTAAACAAAAACAAAGCTTGGAACAACTCACCGCTGCCTTTAATAAAGAGCAGGAAGCGGTAGAGCGTGATTGGAAGGATCGCTTAGCTAACTTAGAAAAGCAAAAGGAAATGCTGGGGGATAAGGTACACCAGTTTGATGAAAATTCGCTCAATATTTCCAAAAAGCTAGCGGAGCGGGAGCTAGCGATTAAATTTAAAGAAAAGGAGCTGGAGGCTGCACAAAAACAGCTAAGTTTAGACAACAACAATAACGCTGGGTTAAAGTTGCAGTTAGATAAATTGAGCGAATCACTCAAAACGGAACGCCTGGAATTAGAAGCATCTAAAGAACGTATTTTGGATTTTTACGATGAATCATCACGCCGAATTGCAGATTATGAAAGCGATTTACAAGCACGCTTAGCTGAGGTTAAGACACTAGAAAAAAACCAGCAGGAAACAGCGGCTAAAAGTGAGCGCGAACTCAAAGTAGCCTTAGAAAAGTTAAACCAAGCAAAAAAGGCTTTTTTACAAATTCGCAAACAGCAATTGCTCGAAATTGCTTCTGTAAAACAACAACTAGCACAAAAAGCTAATTTGTTGAAAAACCAACAAGCAGAGCTAGATAAGCAAACAGAAGAGTTAGAAGCAGCTTTTTTAGAGCAAGATACGGACAAAAAGGAGTTGGAAAAAGCACTCCACAGCGTTAAGTCCAAGCAAGAATTATTAGAGCGCGAACGCAGTTTTTTACTGCAAAAACAACGTGAATTTGCCGAACATGTAGCCGGCTTTAAGCGCCAAGTGCACTTTAAAACAACGCAAATGCAGCGTTTAAGTGAGTTCAACAAACAACAACAAAGTGAACAGATAAAGCGCGAAACGGAGCTCAAAATTGCTTTTGCTGATCTAAAAAAGGATTACCAGCTGTTTGAATTGCAAAAAAACCAGGAGTTCCAGCAAATCGAACAAAAGCACAAAGAACTAGAACTATTAGCACAAAAACAGGCCGAACTCAAACAGGAGTTAGAGCAAAAAGCAACAGCTTTAGCTAGCCAAGATCAAGACACAGTACAGGCCAAGCTAGATTTAGCGCGTCAACAGCATGAATTGGAATTGAAGCAAAATGCCTTTAACCAAGCTAGTCTTTCGCTCAACAAACAACGCGAACAGCTCACAAACCAAGTCAAGGTATTGCACGGTGAGTTGAAAAAACGCCATGAAAAGCTAACTTTAAAGGATCGTTTACTAGCTGAAAAGGAAAAGGATCAGCACAAAAAAGATGCAGAAATTAACCAACGCTTTAAGCAGTTTGAAAATGAATACGCTGATTTTGACCAAGCCAAAAAGCGCGAATTGCAAGAGTTAAACCAAATCCGTCGAAACTTGGAGCAGAGCAATGCATCACTGCTAAAGAAGCGCAACCAACTAACGCTTGATTTTGCTTTACTACGTAAAGTGCAACACAATACGCAAACTAACCGTGTCCAACTCAACACGCAAATTAAGGAGTTTTTACTAGAGAAGAAGAACTTTCAAAAGGCGAGTGATGAAGCCGCTTTACAAAAAGCACTGTTAATTAAACGCTTACGTAGTTTTGCTTCTAAATTACAGTTGCAACGCGAAGCTTTAGCGATTCAAAAACTGGAATTTGATAAGCGTGATGAACAACAAAAGAGTGAGATTAACAACGCTAAGTTACAGTTAGAGCAGTTCAAACTAGAAAAGCAGAACTTTGATGAAGCGAAACAAAAGCAGTTAATTGAGTTTAAGGATCAGTGCCAACGACTTGATGTTGAAAAACGTCTGTTAAAGCAAAAGTTAGTGCAGCTCAAAAACCTATCTAAGAGCTATCTTACTTACAAAAACCGTGCTGATCTGTCCCAACAACAGCTGCAACACAAGTATGCTAACTTACTGGAGTTGAAGGAAAAGCTGCAAACCGCTAAGCGTGCCTTGGACAAAAAACACCGTGCCATTTATGGCAAAATGGCACAGTTTGTCAGTGAGCTCCGCCAAGAAAAGAAGCAGCTTTTAAGTGCGCAAAAACAAGTTGATGACAAGTCACGTTTACTGGAACAAAACCAACGCCATTTACAAAACCTTTCCAGTGAAACCAAAAAGAAACGCCAGTCTTTAGAGCATGACATTAATAAGTTTGATCAAAGACGCAAGGAAGCGGTTTCTTCCATTTTAAACTCGCACAAAAAACTCAAGCAAAAAGAGGGAGAATTGCAAGGCATTTTGCAAAAGCTTAGCTTGAAAAAAACACAAATTGAACAAGAGTTTTCCAAGCTTTATCAGCAACGCGAAAAGTTAGATCGCCAACGCACTACGCTCTCTAAACTCCACAGAGAGCTAAAAGCCCAAAATGAAGCTACGGCACACAAAAATCGTGAAGTTTTAGAGATTGAGAACTATTACAAGAAGGAGTTGCAACGACTCACCACGGAAAAGAGTGAGTTTGATAACAACAAAAATCGCTTGTTTGAATACTTCCGTAAAATTCGCAATGAAATTGAAAAGAAGGAAGCACACATTAAAACCGTTTTAGAAGAAACACAAAAAAAGCGTCACCTAGTCGAAACGGAAGCGGTCAAACTACATTTACAAAAGCAATCTATCATTTCTAAGGGTCAAGAACTTAAAGAAATCAAGGAACGGGTTAGCCGTGACATTAGCCACACCAACAAACAACGCGAAGAGTTAAACAGCTTATTGCACCAAAACAAACTGTTGCAAAAGAATTTGGCTGAGCGTGAACGTGAAATTAATAATAAGGATTCACTGTTAACCCAAAAGATTCAAACAGCTAAACAAAAGTTAAGCGAAAAAGAAGCACGCATCTTAAAGCTGTTGGAAAAAATGCGTGCGGTAGAACAGCAATACCAAGCGGAAATTACCCGTTTAAAGACACGTAATGCTGATTTGGAGAAGAATGACAATAAGCACTTATTTCCACCGTTATTTAAAATTAACGGTAATGATATGAACTATCCTTACCCATATCCTTGA", "aa": "MEARTQFDNRVSLLSARFKQKQAELVKQKQSLEQLTAAFNKEQEAVERDWKDRLANLEKQKEMLGDKVHQFDENSLNISKKLAERELAIKFKEKELEAAQKQLSLDNNNNAGLKLQLDKLSESLKTERLELEASKERILDFYDESSRRIADYESDLQARLAEVKTLEKNQQETAAKSERELKVALEKLNQAKKAFLQIRKQQLLEIASVKQQLAQKANLLKNQQAELDKQTEELEAAFLEQDTDKKELEKALHSVKSKQELLERERSFLLQKQREFAEHVAGFKRQVHFKTTQMQRLSEFNKQQQSEQIKRETELKIAFADLKKDYQLFELQKNQEFQQIEQKHKELELLAQKQAELKQELEQKATALASQDQDTVQAKLDLARQQHELELKQNAFNQASLSLNKQREQLTNQVKVLHGELKKRHEKLTLKDRLLAEKEKDQHKKDAEINQRFKQFENEYADFDQAKKRELQELNQIRRNLEQSNASLLKKRNQLTLDFALLRKVQHNTQTNRVQLNTQIKEFLLEKKNFQKASDEAALQKALLIKRLRSFASKLQLQREALAIQKLEFDKRDEQQKSEINNAKLQLEQFKLEKQNFDEAKQKQLIEFKDQCQRLDVEKRLLKQKLVQLKNLSKSYLTYKNRADLSQQQLQHKYANLLELKEKLQTAKRALDKKHRAIYGKMAQFVSELRQEKKQLLSAQKQVDDKSRLLEQNQRHLQNLSSETKKKRQSLEHDINKFDQRRKEAVSSILNSHKKLKQKEGELQGILQKLSLKKTQIEQEFSKLYQQREKLDRQRTTLSKLHRELKAQNEATAHKNREVLEIENYYKKELQRLTTEKSEFDNNKNRLFEYFRKIRNEIEKKEAHIKTVLEETQKKRHLVETEAVKLHLQKQSIISKGQELKEIKERVSRDISHTNKQREELNSLLHQNKLLQKNLAEREREINNKDSLLTQKIQTAKQKLSEKEARILKLLEKMRAVEQQYQAEITRLKTRNADLEKNDNKHLFPPLFKINGNDMNYPYPYP", "aa_hexdigest": "554e862b292ba705b0d4e11583b148cd", "start_type": "TTG", "rbs_motif": "AACAA", "genes": [ "hmw2" ], "truncated": "both", "pseudogene": { "start": 365306, "stop": 368976, "observations": { "deletion": [ 368807 ], "mutation": [ 365510, 368672 ], "stop": [ 365510, 368672 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75471", "query_cov": 1.0, "subject_cov": 0.5621562156215621, "identity": 0.985, "score": 1639.0, "evalue": 0.0, "gene_start": 1, "gene_end": 1022, "reference_start": 606, "reference_end": 1627, "reference_sequence": "MNDTDKKFPLQPVYDTGFDDGYLQRDYEKCLESAAANDAQTVELQTQLLAEIKNLENEIKALKAQESRQPDPHNNARIQSLEASLNRLVNEYNNFEFQKNYMVDRVAELNNKARFFKDELKRLQQENAAFVNSRYANWADFQSNYQLKLDQFQALIDQQNQTIKQLNEQIAANQGLIDQNVQRLQQNHSLDQQERDALLYEVDHLYNELYELENQKRLVGIEYEATYQDLVSADAELQNVYETIAQNQANFQKQCDAYWAQLKQVEQQIQTTKQELVDEESTLKVRLNDADFYINSRLAELDDLTSKINERDFVSKEQAQDVKASLANLTKEKERLSAEKDSFERLRNTALNDINRMEQENALFAKHLEQQQYEFERKQQESLLKLETEHKQLQKRIGEFKIESEAKSEALLIQERELLEKRREIDDLLTQASLEYEQQRRTNQVLKEKHRQVQQHFQNLVHAKKKLDQKRHYLAEQKRIDEEQIFKLKEKIATERRELEKLYLVKKQKQDQKENDLLIFEKQLRQYQADFENEIEEKQNELFASQKSLQKSFTQLKNKEAELNQKAQKIAEDWAHLKQNKHHHADLEIFLEGEFNHLQQEKHKLLEARTQFDNRVSLLSARFKQKQAELVKQKQSLEQLTAAFNKEQEAVERDWKDRLANLEKQKEMLGDKVHQFDENSLNISKKLAERELAIKFKEKELEAAQKQLSLDNNNNAGLKLQLDKLSESLKTERLELEASKERILDFYDESSRRIADYESDLQARLAEVKTLEKNQQETAAKSERELKVALEKLNQAKKAFLQIRKQQLLEIASVKQQLAQKANLLKNQQAELDKQTEELEAAFLEQDTDKKELEKALHSVKSKQELLERERSFLLQKQREFAEHVAGFKRQVHFKTTQMQRLSEFNKQQQSEQIKRETELKIAFADLKKDYQLFELQKNQEFQQIEQKHKELELLAQKQAELKQELEQKATALASQDQDTVQAKLDLARQQHELELRQNAFNQASLSLNKQREQLTNQVKVLHGELKKRHEKLTLKDRLLAEKEKDQHKKDAEINQRFKQFENEYADFDQAKKRELQELNQIRRNLEQSNASLLKKRNQLTLDFALLRKVQHNTQTNRVQLNTQIKEFLLEKKNFQKASDEAALQKALLIKRLRSFASKLQLQREALAIQKLEFDKRDEQQKSEINNAKLQLEQFKLEKQNFDEAKQKQLIEFKDQCQRLDVEKRLLKQKLVQLKNLSKSYLTYKNRADLSQQQLQHKYANLLELKEKLQTAKRALDKKHRAIYGKMAQFVSELRQEKKQLLSAQKQVDDKSRLLEQNQRHLQNLSSETKKKRQSLEHDINKFDQRRKEAVSSILNSHKKLKQKEGELQGILQKLSLKKTQIEQEFSKLYQQREKLDRQRTTLSKLHRELKAQNEATAHKNREVLEIENYYKKELQRLTTEKSEFDNNKNRLFEYFRKIRNEIEKKEAHIKTVLEETQKKRHLVETEAVKLHLQKQSIISKGQELKEIKERVSRDISHTNKQREELNSLLHQNKLLQKNLAEREREINNKDSLLTQKIQTAKQKLSEKEARILKLLEKMRAVEQQYQAEITRLKTRNADLEKNDNKHLFPPLFKINGNDMNYPYPYPWFYPQQKQEDSSNQIRHLFEQQLQFMQQRYENELTELRRQRALLEKKLDQIQLESQLSAKKNDFEKVEQMMQKLLEKTEQKLSAFDQKINALAEQINTQKAEHADSEKQQLLLRIEQLEKQNLAQAVQTPQPVQPVVQAPAVVPQVIQPQVVQSQPAFLATQQSISKQQQIAQLNAEINSIKKLIAQKAAK" }, "paralog": false, "identity": 0.9950940310711366, "score": 2157.0, "evalue": 0.0, "description": "Internal stop codon at 365510, 368672. Frameshift due to deletion around 368807. Nonsense mutation around 365510, 368672.", "psc": { "uniref90_id": "UniRef90_P75471", "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "uniref50_id": "UniRef50_P75471", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75471", "UniRef:UniRef50_P75471", "COG:COG1196", "COG:D", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_P75471", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75471" ], "product": "Cytadherence high molecular weight protein 2" } }, "id": "CNOCLNGIAL_690", "locus": "GCA000143945_00663" }, { "type": "cds", "sequence": "contig_1", "start": 368881, "stop": 369246, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAGTTGTTGGAAAAAACTGAGCAAAAACTCAGTGCCTTTGACCAAAAAATTAATGCTTTGGCAGAACAGATTAACACACAAAAAGCAGAGCACGCTGACAGTGAAAAGCAACAGTTGTTACTAAGGATTGAACAGTTAGAAAAGCAAAACCTAGCTCAAGCTGTTCAAACACCCCAACCAGTACAACCAGTGGTACAAGCACCAGCTGTAGTACCCCAAGTGATCCAACCACAGGTAGTGCAGTCCCAACCAGCTTTTTTAGCAACGCAGCAAAGTATTTCTAAACAGCAACAAATCGCGCAATTAAACGCTGAGATCAATAGTATTAAAAAGCTAATTGCCCAAAAAGCAGCTAAATAA", "aa": "MQKLLEKTEQKLSAFDQKINALAEQINTQKAEHADSEKQQLLLRIEQLEKQNLAQAVQTPQPVQPVVQAPAVVPQVIQPQVVQSQPAFLATQQSISKQQQIAQLNAEINSIKKLIAQKAAK", "aa_hexdigest": "cb786ea6cb49ec0d318869eb0d9a0888", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13452.331299999989, "isoelectric_point": 9.30196590423584 }, "id": "CNOCLNGIAL_691", "locus": "GCA000143945_00664" }, { "type": "cds", "sequence": "contig_1", "start": 369261, "stop": 370334, "strand": "+", "frame": 3, "gene": null, "product": "putative MG218-1-like protein", "db_xrefs": [ "RefSeq:WP_014325454.1", "SO:0001217", "UniParc:UPI0001DD6523", "UniRef:UniRef100_A0AAV5N9F2", "UniRef:UniRef50_Q9ZB78", "UniRef:UniRef90_P75470" ], "nt": "ATGACTAATGATTACCAACAATTAAATTATTTAGTGGAAACTGATGATGAAGCTGACATCATCATAGCTAACCTCGTCAAACAACTCAATGAATTGAAGGAAATTCTGCTTTCATTGGACAATCAAGATTTAGAAATTAATCAGGTTAACCATCGCACCACAGTGCACAATACTAGTTCTAACACTTCTAATAATAGCACTAGCAATAATAGTGCTGATGGTAATTACAACAGCAATTTCTTTCACAACTTTAGTAAAGAAACGCTGCAGACACAAGCAAAACGTGGCTTTCTTTTACTAGAACGCTGTTCCTTGGTAGGTCTACAACAATTGGAGTTGGAATACCTCAACGTATTGGGTCGTAGCTTTGAATCACATCAAGACAAAATTAACTTTTTGGTCAATTTACGTGATTTAACTAACGATCACTTGTTGGACACGGAAAAGATTGTCAGTACCCTAGAGCAGATCTTTAACGTTATTGGTGGCACGGAATACACCCCGATTCTCAATTCCTTTTTTAACCAATCACTTAACGATCCTGACCCAATTCAGCGCGAAATTGGACTCAAACAGTTTGTGGCTAACTTACGCCAACGCTTTAAAACGCTATTGCAAAAAACTAACAACTCAATTCAACAGTTAGAAGCGGAAATTCAAATTCCAACTACTCATATTAAGAGTGATGAGGTAATGTTTGGCCCACCGGACATGAACGAACGCTTAGTGTTAAATGACAGTGAAACCGATGCCATTCTACGCTCAATTGAAGCGGAGTTGGAAAGCGCTTTACAAAATAAAAAGCAGGTAGTGGTTACAGCAGTCCCACCAATAGCTGCTAACTTAGCTACCGATAGTATTAGCCAAGAAACTTTAGAACACAACCTTAATAACACTGAAACGGTTAACACCACTACTGTCACGGTAACATCAGCTAGTGAAACTCAAGTGCGTAAACCACAGATTTCATTACGCCCCATTTTTCAAGGCAACTTTCCCAAACGCTTAAGTAGAGAAGACATCCAGCGCTATGCCCACCAGTTACAGGAATTAGAACAAAGTAATGAAGGATAG", "aa": "MTNDYQQLNYLVETDDEADIIIANLVKQLNELKEILLSLDNQDLEINQVNHRTTVHNTSSNTSNNSTSNNSADGNYNSNFFHNFSKETLQTQAKRGFLLLERCSLVGLQQLELEYLNVLGRSFESHQDKINFLVNLRDLTNDHLLDTEKIVSTLEQIFNVIGGTEYTPILNSFFNQSLNDPDPIQREIGLKQFVANLRQRFKTLLQKTNNSIQQLEAEIQIPTTHIKSDEVMFGPPDMNERLVLNDSETDAILRSIEAELESALQNKKQVVVTAVPPIAANLATDSISQETLEHNLNNTETVNTTTVTVTSASETQVRKPQISLRPIFQGNFPKRLSREDIQRYAHQLQELEQSNEG", "aa_hexdigest": "944df2fbb3876d7242ffc8a48bc88855", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI0001DD6523", "ncbi_nrp_id": "WP_014325454.1", "uniref100_id": "UniRef100_A0AAV5N9F2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6523", "RefSeq:WP_014325454.1", "UniRef:UniRef100_A0AAV5N9F2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9F2", "uniref90_id": "UniRef90_P75470" }, "psc": { "uniref90_id": "UniRef90_P75470", "product": "Uncharacterized protein MG218.1 homolog", "uniref50_id": "UniRef50_Q9ZB78" }, "pscc": { "uniref50_id": "UniRef50_Q9ZB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB78" ], "product": "Uncharacterized protein MG218.1" }, "genes": [], "id": "CNOCLNGIAL_692", "locus": "GCA000143945_00665" }, { "type": "cds", "sequence": "contig_1", "start": 370324, "stop": 370878, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75469", "UniRef:UniRef90_P75469" ], "nt": "ATGAAGGATAGTGCACTAACACTAAAACGGGTACGAATTGGAAAATTTTCCGAGTCAATGGTTGAAGAACGACCAACACTCAACCTGTTCGAAAAGGTCGAATTCAACCCAGTACCGACTGCTTTAGTGGATCAATTGCCAACTGAACCACTTGTTGAAGCAACTCTTTTAGAAAAGGAAGCAATTACTTTTGTCGATACTTACGCAACTTCGGAAGCTCATGATCAAATAGCTACCTTTGTTTTAGAACAATCAATGGAAACGGAGGTGGTTGAAAAAGAAGCGATTGAAACAGCAATAGTTGCACCAGCACCAGACTTAGTTGAAGAGAAAGCTGTTTTAGTGGAAGAGGTTTTAGTTGAACCAACTGCAACGGAAGCAGTTACAACTGAAGAAAATCAAGTTTCAACTACATCCGTAACTAAGATTAAAACTAAGCGCAGCAATACTAAAAAGGTGACATCAGAAACACTTGTGGCTTCTAAAAGTGTGAAAACTAAAAAGTTAATTACCCCTAACCGGGTAAGTTCGGGCAACGTTAATATAACACTTTGA", "aa": "MKDSALTLKRVRIGKFSESMVEERPTLNLFEKVEFNPVPTALVDQLPTEPLVEATLLEKEAITFVDTYATSEAHDQIATFVLEQSMETEVVEKEAIETAIVAPAPDLVEEKAVLVEEVLVEPTATEAVTTEENQVSTTSVTKIKTKRSNTKKVTSETLVASKSVKTKKLITPNRVSSGNVNITL", "aa_hexdigest": "5b386ea693dd4068c2a82f5e25a6b8e8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75469", "query_cov": 1.0, "subject_cov": 0.8440366972477065, "identity": 1.0, "score": 270.0, "evalue": 1.55e-89, "valid": true, "product": "Uncharacterized protein MPN_312", "uniref50_id": "UniRef50_P75469" }, "pscc": { "uniref50_id": "UniRef50_P75469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75469" ], "product": "Uncharacterized protein MPN_312" }, "genes": [], "id": "CNOCLNGIAL_693", "locus": "GCA000143945_00666" }, { "type": "cds", "sequence": "contig_1", "start": 371063, "stop": 371257, "strand": "-", "frame": 2, "gene": null, "product": "MPN313 family protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniParc:UPI0001DD6525", "UniRef:UniRef100_P75468", "UniRef:UniRef50_Q49403", "UniRef:UniRef90_P75468" ], "nt": "ATGATTGTAAACGCCTTTAGTACGTTAATTAATACCGCGTTAGACATCAGCGAAGCTGCTAACGCTAATAATGCCAACGGCAATGGCAGCAGTTATAGCTATAAAAGAAGGAATTCACAAAAAGACTACTTTAGTACCGGTCGGTTCCGATTAGGTTTAACCCCTGGTAAATCGTCCTATTCTTTTCCCGTTTAA", "aa": "MIVNAFSTLINTALDISEAANANNANGNGSSYSYKRRNSQKDYFSTGRFRLGLTPGKSSYSFPV", "aa_hexdigest": "faaba2bcf9aacc473b9113f13bb76ee0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6525", "uniref100_id": "UniRef100_P75468", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6525", "UniRef:UniRef100_P75468" ] }, "ips": { "uniref100_id": "UniRef100_P75468", "uniref90_id": "UniRef90_P75468" }, "psc": { "uniref90_id": "UniRef90_P75468", "product": "MPN313 family protein", "uniref50_id": "UniRef50_Q49403", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q49403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49403" ], "product": "Uncharacterized protein MG220" }, "genes": [], "id": "CNOCLNGIAL_694", "locus": "GCA000143945_00667" }, { "type": "tRNA", "sequence": "contig_1", "start": 371405, "stop": 371481, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(gcg)", "amino_acid": "Arg", "anti_codon": "gcg", "score": 65.2, "nt": "GTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 371439, 371441 ], "id": "CNOCLNGIAL_9", "locus": "GCA000143945_00668" }, { "type": "cds", "sequence": "contig_1", "start": 371735, "stop": 372160, "strand": "+", "frame": 2, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143", "RefSeq:WP_010874670.1", "SO:0001217", "UniParc:UPI000012F547", "UniRef:UniRef100_P75467", "UniRef:UniRef50_P47463", "UniRef:UniRef90_P75467" ], "nt": "ATGCTTTTAGGAACCTTCAACATTACCCTTGATGCAAAAAACAGGATCAGTCTGCCAGCGAAGCTGCGGGCTTTCTTTGAAGGTAGCATTGTTATCAACCGTGGCTTTGAAAACTGCTTGGAAGTGCGTAAACCACAAGACTTCCAAAAGTACTTTGAGCAGTTCAACAGCTTTCCCTCCACCCAAAAAGACACTAGAACACTCAAGCGCTTAATTTTTGCCAACGCTAACTTTGTGGATGTAGATACAGCGGGACGGGTTTTAATTCCCAACAATCTGATTAACGATGCCAAGCTAGACAAGGAAATTGTTTTAATTGGCCAGTTTGACCACTTAGAGATTTGGGACAAGAAGCTGTATGAGGACTACCTAGCCAACTCTGAGTCGTTGGAAACGGTAGCGGAAAGGATGAAAGATGTTAAGTAA", "aa": "MLLGTFNITLDAKNRISLPAKLRAFFEGSIVINRGFENCLEVRKPQDFQKYFEQFNSFPSTQKDTRTLKRLIFANANFVDVDTAGRVLIPNNLINDAKLDKEIVLIGQFDHLEIWDKKLYEDYLANSESLETVAERMKDVK", "aa_hexdigest": "8b2975df80e77adad7ccac97fe29d2f1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012F547", "ncbi_nrp_id": "WP_010874670.1", "uniref100_id": "UniRef100_P75467", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012F547", "RefSeq:WP_010874670.1", "UniRef:UniRef100_P75467" ] }, "ips": { "uniref100_id": "UniRef100_P75467", "uniref90_id": "UniRef90_P75467" }, "psc": { "uniref90_id": "UniRef90_P75467", "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "uniref50_id": "UniRef50_P47463", "cog_id": "COG2001", "cog_category": "J", "go_ids": [ "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143" ] }, "pscc": { "uniref50_id": "UniRef50_P47463", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47463" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "CNOCLNGIAL_695", "locus": "GCA000143945_00669" }, { "type": "cds", "sequence": "contig_1", "start": 372150, "stop": 373076, "strand": "+", "frame": 3, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424", "RefSeq:WP_014325456.1", "SO:0001217", "UniParc:UPI0001DD6526", "UniRef:UniRef100_A0AAV5NAY0", "UniRef:UniRef50_P47464", "UniRef:UniRef90_P75466" ], "nt": "ATGTTAAGTAATCAACCCCACAAAAGTGTCTTACTTGATGAAGTCATCCACAACCTCAACATTAAAGGTGATGGTAACTATTTAGATCTTACTGTTGGTTTTGGCGGTCATAGTCAGCACATTTTAGAGCAGCTTACCACTGGCACTTTAACTGGCAATGACATGGATAAGGACAGCATTACCTTTTGTACCGAGCTGTTCAAAGACAAAAAGAACGTTGTTTTAGTCCATGATAACTTCGCTAACTTTTTTAACCACTTAAAGCAGCTCAAAGTAACGAAGTTTGACGGCATCTTAATTGATTTAGGGGTTTCGAGCTACCAGCTAGATAAACCAGAACGCGGTTTTAGTTTTAAACACGCCGGTCCATTTGACATGCGCATGCACCAAAGCGATCGCGTACCAACTGCTTTAGATATCTTGGAGAGGTTGTCTGAGGAAGAATTGACACATGTTTTAAAGAAGTTTGGTGAAATAGCCCACCCCAAACCAATCGCCAAGGCCTTAAAAACACTCATTAATAACATTAAAAATCCAACTACGGTAGAGGTAGCGGAAACAGTTAAGGCAGCTGCTAGCAACTTTGAAAAGTACAAATCACGCAATTACTTAGCTAAAGTTTTCCAAGCCATTCGCATTTACCTCAACCGTGAACTGGAAAGTTTGGAAATTGTGCTGCAACACATTCCCAAGCTGTTGAACAATAAGGGTCGTTTTCTAGTGATTGTCTTCCATTCACTCGAGGAAAAATTGGTGCGGAACTACATTTTTAAACTTACTCACTTTGTCCAACCCCCTGAACTACCCGTTAAATTAACCCCACCGTTTGAACTCATTACTAAAAAACCCATTTTGCCAACTGAACAAGAAATTAAAACGAACCCCCGTGCTCGAAGTGCCAAACTGTTTGTCATTGAAAAGAAATAA", "aa": "MLSNQPHKSVLLDEVIHNLNIKGDGNYLDLTVGFGGHSQHILEQLTTGTLTGNDMDKDSITFCTELFKDKKNVVLVHDNFANFFNHLKQLKVTKFDGILIDLGVSSYQLDKPERGFSFKHAGPFDMRMHQSDRVPTALDILERLSEEELTHVLKKFGEIAHPKPIAKALKTLINNIKNPTTVEVAETVKAAASNFEKYKSRNYLAKVFQAIRIYLNRELESLEIVLQHIPKLLNNKGRFLVIVFHSLEEKLVRNYIFKLTHFVQPPELPVKLTPPFELITKKPILPTEQEIKTNPRARSAKLFVIEKK", "aa_hexdigest": "847daf484b3924d2245557a8b918bf52", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6526", "ncbi_nrp_id": "WP_014325456.1", "uniref100_id": "UniRef100_A0AAV5NAY0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6526", "RefSeq:WP_014325456.1", "UniRef:UniRef100_A0AAV5NAY0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NAY0", "uniref90_id": "UniRef90_P75466" }, "psc": { "uniref90_id": "UniRef90_P75466", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_P47464", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ] }, "pscc": { "uniref50_id": "UniRef50_P47464", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47464" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "CNOCLNGIAL_696", "locus": "GCA000143945_00670" }, { "type": "cds", "sequence": "contig_1", "start": 373079, "stop": 374194, "strand": "+", "frame": 2, "gene": null, "product": "MPN316 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465", "UniRef:UniRef90_P75465" ], "nt": "ATGTATAACCTCAAAAACATCTACGCCTCCATTACCCTTTACCCCCATGAAATTAACTTGGTGGTATCGGACAACAACAATCAGTTTCATGTCTTGTATGAAAACTCGATTGCTAACGATGAGCTTTACACGCACGCGGGCATTACTAATAAGGCAAAGTTCAAGGTAGTTTTAAACCAGCTCATTAACAATGCAAACGATTATTTGGGTTTTAAACTCGAAAAAGTAATTGTGGTTTTAGCGGAGTTAGTTGATGATCTTGAAATTAACCGCTTTAAAACAGATGTCTTTTTCACTGGTTATGACTTTAACCACCAGGACATGTTGAGCAGTGAAAAGAACCGCTTCTTGCTCAAAAACCGTCATATCAACCCCAATGAAGTTTTGGACTTAGTGGCTTTAAACTACCGTGATTTAACCACTGGTAAAATTAGCAAAAACTTTAAGTACAACTGCAGTTATCGGGCAAATGTAATTTATTACACCACTAAAAACTCACTAGTCAAGGAGCTCAAACCGTTTTTAAAGCGCAATATTATTGTCAAAATTGACAAAATTGTCACCCACCACATGGTGTTAGCCCACTCAATTAAACAAATCAACAAAAACAACCTCTTTGTGTATTTAGGGGAACACACTACTGACCTCATGTTGTTTATGGACAATGCCTTAGTTGATTTAGTAAGTGAACCCTTTGGTCGGGTTAACTTCTTGGAATCTGACAATGACAGTGAAAACAAAGCGCTGTTGGAATTCCTAGTGGACTCTACTGCACGCATTGGTGACTCGGGCTCGATTGGCATGGCTTACACCGATGGTTCTACTTATAAGGAAATTAGAGCTGTTACGATTGATGACCTCATTCAAAGTGTTAATGAAAAAACGAAGTACTTAATTGATTTCATTAACACTAACACGGAAATCTTCTTCCAAAAGTACGGCTTTTTACCCAGCACTTTACTGTTTTACACTAAGAGTAAACAGTTGCTTAACAACTTTCAACTCAACCAAAACCTGTTGAGTAACCACTTCAAAACTGTAACCATCTTTAAAAACGAAGTTCAGTTTGTGAGTCGCAAATACTTATTGAACTGTCAAGCAATTGCGCTTTCTTGA", "aa": "MYNLKNIYASITLYPHEINLVVSDNNNQFHVLYENSIANDELYTHAGITNKAKFKVVLNQLINNANDYLGFKLEKVIVVLAELVDDLEINRFKTDVFFTGYDFNHQDMLSSEKNRFLLKNRHINPNEVLDLVALNYRDLTTGKISKNFKYNCSYRANVIYYTTKNSLVKELKPFLKRNIIVKIDKIVTHHMVLAHSIKQINKNNLFVYLGEHTTDLMLFMDNALVDLVSEPFGRVNFLESDNDSENKALLEFLVDSTARIGDSGSIGMAYTDGSTYKEIRAVTIDDLIQSVNEKTKYLIDFINTNTEIFFQKYGFLPSTLLFYTKSKQLLNNFQLNQNLLSNHFKTVTIFKNEVQFVSRKYLLNCQAIALS", "aa_hexdigest": "1d06c9d7e5ad8a72784804a53d16e1cc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75465", "query_cov": 1.0026954177897573, "subject_cov": 0.8857142857142857, "identity": 0.997, "score": 723.0, "evalue": 2.2e-262, "valid": true, "product": "MPN316 family protein", "uniref50_id": "UniRef50_P47465" }, "pscc": { "uniref50_id": "UniRef50_P47465", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465" ], "product": "Uncharacterized protein MG223" }, "genes": [], "id": "CNOCLNGIAL_697", "locus": "GCA000143945_00671" }, { "type": "cds", "sequence": "contig_1", "start": 374449, "stop": 375096, "strand": "+", "frame": 1, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "GO:0000910", "GO:0003924", "GO:0005525", "KEGG:K03531", "SO:0001217", "UniRef:UniRef50_A0AB38W9V7", "UniRef:UniRef90_A0AB38W9V7" ], "nt": "ATGTTGCGCATGCACCCCGAATTGCAAACGGCTAACGTCCAGTTCTTTGCTTTAAACACTGATTTACAGCACTTAAAAACAAAGCGCTATGTCCAAAATAAGGCTGTGATTCAGTTTGAAGAATCCAAGGGCTTAGGTGTTGGTGGTGATCCCCAAAAAGGTGCCGTTTTAGCACACCATTTTTTAGAGCAATTTCACAAACTTAGTGACAGCTTTGATTTCTGTATTTTAGTCGCTGGTTTTGGTAAGGGGACTGGTACTGGTGCTACCCCGGTTTTTAGTAAGTTTCTTAGCAACAAAGGGGTGTTAAATCTCTCGATTGTCAGTTACCCCGCAATGTGTGAAGGGTTAAAGGCACGCGAAAAAGCAGCTAAAGGTTTAGAACGCTTAAACCAAGCGACTGACAGTTTTATGCTGTTCCGCAATGATCGCTGTACTGATGGCATTTACCAACTAGCAAACGTTGCCATTGTCAAAACAATTAAAAACATTATTGAACTAATTAATCTACCACTACAGCAAAACATTGATTTTGAAGACATTCGTTCTTTCTTTAAAAAACCAGCACAACGTTTAGAGAATGAAGCTAACTTGTTTCGCGTAACTAATACTTTTACTTTTAGCTTTAGACGCGCACAATACAATTGA", "aa": "MLRMHPELQTANVQFFALNTDLQHLKTKRYVQNKAVIQFEESKGLGVGGDPQKGAVLAHHFLEQFHKLSDSFDFCILVAGFGKGTGTGATPVFSKFLSNKGVLNLSIVSYPAMCEGLKAREKAAKGLERLNQATDSFMLFRNDRCTDGIYQLANVAIVKTIKNIIELINLPLQQNIDFEDIRSFFKKPAQRLENEANLFRVTNTFTFSFRRAQYN", "aa_hexdigest": "1586323afe4024ecc62370d15f90a7af", "start_type": "ATG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_A0AB38W9V7", "query_cov": 1.0, "subject_cov": 0.849802371541502, "identity": 1.0, "score": 429.0, "evalue": 2.28e-151, "valid": true, "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_A0AB38W9V7", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W9V7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W9V7" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "CNOCLNGIAL_698", "locus": "GCA000143945_00672" }, { "type": "cds", "sequence": "contig_1", "start": 375113, "stop": 375241, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAACTTTGAGTATGAAGGTTTTTTTGACCATAAGGTAGAAGGGGCGCAAAAGGTAATTTTAAAGGTGTTAGTCAACCAAGGTTTATATCCGCTTGATTTAACCCAAATTCAAGAGATTATTTGA", "aa": "MKNFEYEGFFDHKVEGAQKVILKVLVNQGLYPLDLTQIQEII", "aa_hexdigest": "b3e1d0a20e20a99572445e3dbf18a3f6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4909.653699999999, "isoelectric_point": 5.004696083068848 }, "id": "CNOCLNGIAL_699", "locus": "GCA000143945_00673" }, { "type": "cds", "sequence": "contig_1", "start": 375308, "stop": 375487, "strand": "+", "frame": 2, "gene": "ftsZ", "product": "Cell division protein FtsZ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8D0", "UniRef:UniRef90_A0AB38W8D0" ], "nt": "GTGCAACTGTTTTTCTTAATGGAGAAAAAGCAAGCAGTGTCCTCTGACTTTATTCAAAAACCAGCATTTATCTCAGTTAAAGAAGTAAATCAGAAACCGGCCAAACCGTTCCAAGTTTTGAATGATTTAAAGGAGCTGGGACTGAAATACGTCAAACAGCAAACCGGATTTAATTATTAG", "aa": "MQLFFLMEKKQAVSSDFIQKPAFISVKEVNQKPAKPFQVLNDLKELGLKYVKQQTGFNY", "aa_hexdigest": "16a3b4888b1450bbcb56766c25c74535", "start_type": "GTG", "rbs_motif": null, "genes": [ "ftsZ" ], "truncated": "5-prime", "pseudogene": { "start": 375113, "stop": 375487, "observations": { "mutation": [ 375239 ], "stop": [ 375239 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8D0", "query_cov": 0.9830508474576272, "subject_cov": 0.46774193548387094, "identity": 1.0, "score": 115.0, "evalue": 7.25e-32, "gene_start": 2, "gene_end": 59, "reference_start": 67, "reference_end": 124, "reference_sequence": "MKNFEYEGFFDHKVEGAQKVILKVLVNQGLYPLDLTQIQEIIWAKIDNHNLEVQLGVDFTDANPSVQLFFLMEKKQAVSSDFIQKPAFISVKEVNQKPAKPFQVLNDLKELGLKYVKQQTGFNY" }, "paralog": false, "identity": 0.9838709677419355, "score": 243.0, "evalue": 6.15e-85, "description": "Internal stop codon at 375239. Nonsense mutation around 375239.", "psc": { "uniref90_id": "UniRef90_A0AB38W8D0", "product": "Cell division protein FtsZ", "uniref50_id": "UniRef50_A0AB38W8D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8D0", "UniRef:UniRef50_A0AB38W8D0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8D0" ], "product": "Cell division protein FtsZ" } }, "id": "CNOCLNGIAL_700", "locus": "GCA000143945_00674" }, { "type": "cds", "sequence": "contig_1", "start": 375530, "stop": 375652, "strand": "+", "frame": 2, "gene": null, "product": "amino acid permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8", "UniRef:UniRef90_UPI0027DFE8A8" ], "nt": "ATGGCTGTTGCAAGTAAGAAGTTCACTGAACGTCAATTTTTATTTTTTGTTGTCAACTATATTGCTGGCTTTGGTTTTATTTCCACTGCCCTATCAATGTTTGAGCTAGGACCACTTTCATGA", "aa": "MAVASKKFTERQFLFFVVNYIAGFGFISTALSMFELGPLS", "aa_hexdigest": "77739137e25960ae4ccafcec55728646", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 375530, "stop": 375796, "observations": { "mutation": [ 375650, 375752 ], "stop": [ 375650, 375752 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0027DFE8A8", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 0.975, "score": 79.3, "evalue": 1.68e-18, "gene_start": 1, "gene_end": 40, "reference_start": 1, "reference_end": 40, "reference_sequence": "MAVASKKFTERQFLFFVVNYIAGFGFISTALSLFELGPLSWVIFFIVSLISLIVTLAFARVSAIDKNNYGGAYLWAKKAFNSERYSHR" }, "paralog": false, "identity": 0.9545454545454546, "score": 162.0, "evalue": 1.05e-53, "description": "Internal stop codon at 375650, 375752. Nonsense mutation around 375650, 375752.", "psc": { "uniref90_id": "UniRef90_UPI0027DFE8A8", "product": "amino acid permease", "uniref50_id": "UniRef50_UPI0027DFE8A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0027DFE8A8", "UniRef:UniRef50_UPI0027DFE8A8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0027DFE8A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8" ], "product": "amino acid permease" } }, "id": "CNOCLNGIAL_701", "locus": "GCA000143945_00675" }, { "type": "cds", "sequence": "contig_1", "start": 376034, "stop": 377005, "strand": "+", "frame": 2, "gene": null, "product": "APC family permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2", "UniRef:UniRef90_UPI001FB1D5B2" ], "nt": "GTGCTTTTGAGCACCTTAATAGTAGCACTTATAGTTATCGGCAAAAATGGTAATGGTTATGCCATTAACAACGACATAAAACAAGGCCCTTTTAAAGAACGTGAAATTACCTTGGGTGCAGTTGCTACTGTCTTTATTACCTTCTTTTATTCCTACAGTGGTGCGGAGGACATTTCAACAATGACACCGGATGTGAAAACCACTAACTTCCGCAAGATTTTAATCATCTCTTTTATTGCGGTATTCTTGTTTTACTTTGTGGGCATTTTGATCTTTAATGGTTTATTGAATGTTTCACAAAGCGATGATGATAAGGGGAAAGATGGAAGAATAGCTAGTGTAGCTGATATTTTTAGATTAGCTGGCGGTTTAGGGGCACTCATTTTTTACGGTGTGGGTGCTTTATTCAATAACGTATCTACCAGACTTTCGACCATTATTGCTAACTCACGCAAGATCTTACCGTTAGCACAAGATAACTATTTACCCACTACCTTTAGCAAAGCTAACAGTAAGGGCGAGTTCCAAAATGCAATTTGGTTTACCTTTGGTACTACCATCATTACGATGACGGTACTGTTCTTTGTTCCGCTAATAACCAGCAACTTTGACTTCGACAAATCAACCGAGTATGCCGCCTCGATAGGTTCGGCCGCTACACTGGTACAATATATCCTTGTATTTTTTATAATTTTTAGGCTTATTTATAAAAAAGAGCAACTTTATCAAAAAAACTGGACACGCGAGTTAGAACAAATACTGTTTGGGATTGGTGTCTTTATTATTCTTCTCATGTTGCTCTTTTATCTCTTTCCAGTAATTGATGGAAAAACTAAATGGCAATTGAAAAATACCCTCACTTTAGTGGTGTATGGGGTGTTGATTTTAATTGGATTTGCCCTCTTTATGACCCAAGAATATAAGCGCAATAAACAAAAGGAAAGCTATGTCACACCAAGAGCAGTTACATAA", "aa": "MLLSTLIVALIVIGKNGNGYAINNDIKQGPFKEREITLGAVATVFITFFYSYSGAEDISTMTPDVKTTNFRKILIISFIAVFLFYFVGILIFNGLLNVSQSDDDKGKDGRIASVADIFRLAGGLGALIFYGVGALFNNVSTRLSTIIANSRKILPLAQDNYLPTTFSKANSKGEFQNAIWFTFGTTIITMTVLFFVPLITSNFDFDKSTEYAASIGSAATLVQYILVFFIIFRLIYKKEQLYQKNWTRELEQILFGIGVFIILLMLLFYLFPVIDGKTKWQLKNTLTLVVYGVLILIGFALFMTQEYKRNKQKESYVTPRAVT", "aa_hexdigest": "eb16bf6966326977e9ed64777290fb65", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FB1D5B2", "query_cov": 1.0, "subject_cov": 0.8801089918256131, "identity": 0.9940000000000001, "score": 613.0, "evalue": 8.3e-221, "valid": true, "product": "APC family permease", "uniref50_id": "UniRef50_UPI001FB1D5B2" }, "pscc": { "uniref50_id": "UniRef50_UPI001FB1D5B2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2" ], "product": "APC family permease" }, "genes": [], "id": "CNOCLNGIAL_702", "locus": "GCA000143945_00676" }, { "type": "cds", "sequence": "contig_1", "start": 376980, "stop": 377291, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D8CD42" ], "nt": "ATGTCACACCAAGAGCAGTTACATAAACCTAACCGGCAACAGTTTAGCGAAAAGCAATTTATTGCTTTTGCTTTTAACTATGTTGCTGGGTTCGGCTTTATTTCCGTGGTGCTAACCATGTTTAAGCTTGGCCCGTTTTCTTACTTGATCCTAGGGCTAGCAGCTTTAGGTATTTTGGGGGTAATGTTGTCCTTTTCGCGCCTTTCAATTATTTGTGGGAGCAAGGCTTACGGTGGTAGTTATTTAATAGCCAAAAAAGCACTTGGTGCGAAAACAATCACGGCGCGTTTCTTTACTTTTTTGAGTGGTTGA", "aa": "MSHQEQLHKPNRQQFSEKQFIAFAFNYVAGFGFISVVLTMFKLGPFSYLILGLAALGILGVMLSFSRLSIICGSKAYGGSYLIAKKALGAKTITARFFTFLSG", "aa_hexdigest": "af51c3148a720006783fe73542ca4d54", "start_type": "ATG", "rbs_motif": "AATAA", "psc": { "uniref90_id": "UniRef90_UPI0002D8CD42", "query_cov": 0.9223300970873787, "subject_cov": 0.8878504672897196, "identity": 0.705, "score": 134.0, "evalue": 6.51e-39, "valid": false, "uniref50_id": "UniRef50_UPI0002D8CD42" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D8CD42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D8CD42" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11281.24149999998, "isoelectric_point": 10.08564357757568 }, "id": "CNOCLNGIAL_703", "locus": "GCA000143945_00677" }, { "type": "cds", "sequence": "contig_1", "start": 377328, "stop": 377504, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAGCGGTGCTAGTTCTCTCGTTTGCTGATATTAAAGCAGTGAAGGATAACAATGGTGCTTTAATTGGCTTACTGGTCGGGGGCTTTGTTTTATTTGGGGCATTAAACTTCATCTCCCTGTTTGGCCTTAAAATGAATAAGAACGCCATTCTCTTCTTTGCCATAGTGAAGTGA", "aa": "MPAVLVLSFADIKAVKDNNGALIGLLVGGFVLFGALNFISLFGLKMNKNAILFFAIVK", "aa_hexdigest": "ef8547207aa41428e2ef7e35cd19c12f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6155.446099999997, "isoelectric_point": 9.82448215484619 }, "id": "CNOCLNGIAL_704", "locus": "GCA000143945_00678" }, { "type": "cds", "sequence": "contig_1", "start": 377529, "stop": 378059, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00324.27" ], "nt": "TTGGGAATCTACCTCATCGGTACGAACCACGGCCATGGTTTTGTCGAAAACAACACTTTAGGTGAACACATTGAAGATCTCAGTTTCCTTAAAGTTATTTCTACTACTGTGGGGATGTTGGTGGCCTTTGCTGGTACCGAAGATCTTACTGCGATTACCCCGGATGTGAAGTCAAAAAACATCCGTAAGTGTTTCTTATTGATGTTTGGTGCAGTTACCCTGCTTTATCTAATCGGTTTTGTCATTATTAGTGGGATTTCTGGTTTAAATGGTTATGGCCTTGATGGTAAAGAAAAGAATGAAAAAGCGATTAACACCTTTGGTTCGATTTACTTTCAAGCAGGCGGTAAGTATTTAGGCATCCCACTGTTGGTCATCTTTGGTCTGGGTTTTTTACTCAATTCCTTGGCATCACGATTAGGCATGACCATTACGACAGCGCGCAAATACGTTGCGTTAGCCCAAGATGGTTTCTTGCCGTCCTTTATTAACGAACAAAACAAGCACCACGAGTACCATAAGGCAGTGTGA", "aa": "MGIYLIGTNHGHGFVENNTLGEHIEDLSFLKVISTTVGMLVAFAGTEDLTAITPDVKSKNIRKCFLLMFGAVTLLYLIGFVIISGISGLNGYGLDGKEKNEKAINTFGSIYFQAGGKYLGIPLLVIFGLGFLLNSLASRLGMTITTARKYVALAQDGFLPSFINEQNKHHEYHKAV", "aa_hexdigest": "d078806c438e1273d34bd674bb283a15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00324.27", "name": "AA_permease", "length": 169, "aa_cov": 0.9431818181818182, "hmm_cov": 0.9349112426035503, "evalue": 1.6579338198467255e-13, "score": 45.527122497558594, "start": 11, "stop": 176 } ], "seq_stats": { "molecular_weight": 19119.021000000008, "isoelectric_point": 7.866964149475098 }, "id": "CNOCLNGIAL_705", "locus": "GCA000143945_00679" }, { "type": "cds", "sequence": "contig_1", "start": 378072, "stop": 378287, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTTAGCAGTGATGGTGCTGATGATCATTGTCCCCTTTTTACCTAATGATGAAAACCCCGGTAAACAGCTCGTTATGTTCGATGCCATTAGTGTTTTAGTTGAAGTGGCGATTGAACTAGCAGTGTTAATTTCCTTAATCCAGTACTTTATTACCTACATCTTCTTCTTTATGATTCTGGCCAAAAAAGAGGGTAGTGCCAGTGTTTCGTGA", "aa": "MTLAVMVLMIIVPFLPNDENPGKQLVMFDAISVLVEVAIELAVLISLIQYFITYIFFFMILAKKEGSASVS", "aa_hexdigest": "19ea4c89d06288c76d5d0ab287d188c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7897.521499999995, "isoelectric_point": 4.3588346481323255 }, "id": "CNOCLNGIAL_706", "locus": "GCA000143945_00680" }, { "type": "cds", "sequence": "contig_1", "start": 378863, "stop": 379141, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00303.24" ], "nt": "ATGAGCTTTAAAATAACCGTTTTAAGTTATTGCAAAAACTCTTTTAAAAAAGTTATTCGCTTATTTTGCAAGCTTAAAAAAAGATTTTTATACAATGCTACACCATCGAAGTTTAGGAGAGTTATGCAGCAGTATCTAGATTTAGCCCGTTATGTTTTAGAGCACGGAAAATACCGTCCAAATCGCACTGATACTGCTGGCATTGGTGTCTTTGGTTACCAGATGCACTTTGATATTTCAAAACACTTTCCGCTCTTGACTACGAAGAAGGTGCACTGA", "aa": "MSFKITVLSYCKNSFKKVIRLFCKLKKRFLYNATPSKFRRVMQQYLDLARYVLEHGKYRPNRTDTAGIGVFGYQMHFDISKHFPLLTTKKVH", "aa_hexdigest": "8badef1771ba78bade1c445ee768f036", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00303.24", "name": "Thymidylat_synt", "length": 51, "aa_cov": 0.5434782608695652, "hmm_cov": 1.0, "evalue": 3.044769208271441e-15, "score": 51.4938850402832, "start": 43, "stop": 92 } ], "seq_stats": { "molecular_weight": 10995.94419999999, "isoelectric_point": 10.29677753448486 }, "id": "CNOCLNGIAL_707", "locus": "GCA000143945_00681" }, { "type": "cds", "sequence": "contig_1", "start": 379478, "stop": 379849, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00303.24" ], "nt": "ATGTTGCTCCCGCCTTGTCATTCACTGTACCAGTTTTACGTGCAGGACGGGCAGTTGAGCTGCCAGTTGTACCAGCGCAGTGGTGATATTTTTCTAGGTATCCCCTTCAACATTGCGTCCTACTCTCTTCTGGTGTACTTAGTTGCTAAGGAAACTAACCTAAAACCAGGGAGCTTTGTCCACACCATTGGCGATGCCCACATTTACGAAAATCACTTGGAACAAATTAAGTTGCAATTAACACGTCAACCCAAACCATTGCCCAAGGTAGTTTTAAAGAGTGATAAATCAATTTTTGATTATCAATTCGATGATATTGAACTGGTGGATTACGACCACCACCCCACAATTAAAGGAGAGGTAGCAGTTTAA", "aa": "MLLPPCHSLYQFYVQDGQLSCQLYQRSGDIFLGIPFNIASYSLLVYLVAKETNLKPGSFVHTIGDAHIYENHLEQIKLQLTRQPKPLPKVVLKSDKSIFDYQFDDIELVDYDHHPTIKGEVAV", "aa_hexdigest": "299a13719ecd353eabe1e055efd5df75", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00260828A0", "query_cov": 1.008130081300813, "subject_cov": 0.8104575163398693, "identity": 0.637, "score": 174.0, "evalue": 8.86e-54, "valid": false, "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_A0A059X7H5", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0006231" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00303.24", "name": "Thymidylat_synt", "length": 126, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.2897476655412427e-56, "score": 186.31985473632812, "start": 1, "stop": 123 } ], "seq_stats": { "molecular_weight": 14107.032299999983, "isoelectric_point": 5.7831628799438475 }, "id": "CNOCLNGIAL_708", "locus": "GCA000143945_00682" }, { "type": "cds", "sequence": "contig_1", "start": 379974, "stop": 380333, "strand": "+", "frame": 3, "gene": "folA", "product": "Dihydrofolate reductase", "db_xrefs": [ "COG:COG0262", "COG:H", "EC:1.5.1.3", "GO:0004146", "GO:0005829", "GO:0006730", "GO:0046452", "GO:0046654", "GO:0046655", "GO:0050661", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_P78028", "UniRef:UniRef90_P78028" ], "nt": "ATGGGCTCGGCTACCTATCTTTCCTTACCGCCGGTGTTTAGTGAGCGTAACGTTTATATCCTCACCCGTAACCTCAACTTTAACCCGCCTGATAAGGGCTGTTTGACTAAAGTAATCCATGAATATGAAAACTTTATCCAACCTTATTTGCACCATCCCGATAAACACCTCTACATCTGTGGTGGGGCACAAGTTTATGAACAGCTCATTCCCCGGTGTGATGCGTTAATTGTCTCTACCATTTTCGGTAAATACACCGGGGACAAATACCTCAAAGTGGATTTTAGTCCCTTTGAATTGACCAAAGAGATCAGTTTTGCTGAGTTTAAGGTCGCTTACTATCACAAAATTGCACGATAA", "aa": "MGSATYLSLPPVFSERNVYILTRNLNFNPPDKGCLTKVIHEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTIFGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR", "aa_hexdigest": "06c5e173ec0cec6d25ce0aa36513a4ef", "start_type": "ATG", "rbs_motif": null, "genes": [ "folA" ], "truncated": "5-prime", "pseudogene": { "start": 379851, "stop": 380333, "observations": { "mutation": [ 379866, 379914 ], "stop": [ 379866, 379914 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78028", "query_cov": 1.0, "subject_cov": 0.74375, "identity": 1.0, "score": 251.0, "evalue": 3.2e-84, "gene_start": 1, "gene_end": 119, "reference_start": 42, "reference_end": 160, "reference_sequence": "MVKAIWAMDQNGLIGNGNSLPWRIKAELQHFRQTTLHQDVLMGSATYLSLPPVFSERNVYILTRNLNFNPPDKGCLTKVIHEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTIFGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR" }, "paralog": false, "identity": 0.9875, "score": 325.0, "evalue": 1.1e-115, "description": "Internal stop codon at 379866, 379914. Nonsense mutation around 379866, 379914.", "psc": { "uniref90_id": "UniRef90_P78028", "gene": "folA", "product": "Dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_P78028", "cog_id": "COG0262", "cog_category": "H", "kegg_orthology_id": "K00287", "go_ids": [ "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78028", "EC:1.5.1.3", "UniRef:UniRef50_P78028", "COG:COG0262", "COG:H", "KEGG:K00287", "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ] }, "pscc": { "uniref50_id": "UniRef50_P78028", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78028" ], "product": "Dihydrofolate reductase" } }, "id": "CNOCLNGIAL_709", "locus": "GCA000143945_00683" }, { "type": "cds", "sequence": "contig_1", "start": 380340, "stop": 380432, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAACACAAAAAAATACTTTTTAGAATCAGTTTCTCCCTTAGAATATGCACAAAAAAAGTTTCAGGGCAACTTGCGCTCGGTTAACTGA", "aa": "MANTKKYFLESVSPLEYAQKKFQGNLRSVN", "aa_hexdigest": "fa66f01242337ad0ed68da612541c86a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3491.9673000000003, "isoelectric_point": 9.69799518585205 }, "id": "CNOCLNGIAL_710", "locus": "GCA000143945_00684" }, { "type": "cds", "sequence": "contig_1", "start": 380688, "stop": 381350, "strand": "+", "frame": 3, "gene": null, "product": "ribonucleoside-diphosphate reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A376CKW9" ], "nt": "ATGGTGGGGGTACACGCGCGCTCTTATGGCACCATCTTTTCCACTCTGTGTACTTCAGAACAAATTACTGAAGCACATGAATGGGTGGTGAAAACCGAAAGCTTGCAAAAGCGCGCTAAGGCTTTAATTCCCTATTACACCGGGAAAGACCCATTAAAGTCTAAGGTAGCAGCCGCTTTAATGCCCGGGTTCTTACTGTATGGTGGGTTTTATTTACCGTTTTACCTCTCCTCGCGTAAACAGCTACCCAACACTTCGGATATTATTCGCTTAATTTTGCGCGATAAGGTAATCCATAACTATTACAGTGGGTATAAGTTCCAGCGTAAAGTGGAAAAGATGTCCAAGGAAAAACAGGCTGAGATGAAACGGTTTGTCTTTGATTTAATGTACGAACTGATTGAGTTGGAAAAGGCATACCTCAAGGAACTGTATGAAGGGTTTGGGATTGTAGAAGATGCCATTAAATTCAGTATTTATAACGCTGGTAAGTTTTTGCAAAACTTAGGGTATGACTCACCGTTTACAGAAGAAGAAACGCGGATTAAACCAGAAATTTTTGCCCAATTGTCAGCCAGAGCCGATGAAAACCACGATTTCTTTTCTGGTAATGGTTCGTCATATGTGATGGGAATTAGTGAAGAGACCGAAGATAAGGATTGA", "aa": "MVGVHARSYGTIFSTLCTSEQITEAHEWVVKTESLQKRAKALIPYYTGKDPLKSKVAAALMPGFLLYGGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKFQRKVEKMSKEKQAEMKRFVFDLMYELIELEKAYLKELYEGFGIVEDAIKFSIYNAGKFLQNLGYDSPFTEEETRIKPEIFAQLSARADENHDFFSGNGSSYVMGISEETEDKD", "aa_hexdigest": "4567b7e2d05af6d18aceebc0821d9282", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E3ENY0", "query_cov": 1.0, "subject_cov": 0.9565217391304348, "identity": 0.505, "score": 227.0, "evalue": 4.71e-72, "valid": false, "gene": "nrdF", "product": "class 1b ribonucleoside-diphosphate reductase subunit beta", "ec_ids": [ "1.17.4.1" ], "uniref50_id": "UniRef50_A0A376CKW9", "go_ids": [ "GO:0004748", "GO:0005971", "GO:0009263" ] }, "pscc": { "uniref50_id": "UniRef50_A0A376CKW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A376CKW9" ], "product": "ribonucleoside-diphosphate reductase" }, "genes": [], "id": "CNOCLNGIAL_711", "locus": "GCA000143945_00685" }, { "type": "cds", "sequence": "contig_1", "start": 381362, "stop": 381823, "strand": "+", "frame": 2, "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "db_xrefs": [ "COG:COG1780", "COG:F", "GO:0009263", "GO:0010181", "GO:0036211", "KEGG:K03647", "RefSeq:WP_014325461.1", "SO:0001217", "UniParc:UPI0001DD652A", "UniRef:UniRef100_A0AAV5NAT1", "UniRef:UniRef50_P47472", "UniRef:UniRef90_P75460" ], "nt": "ATGCATAAAGATATCAAAATAGTCGACGCTAGTGCGATCGTTAAACCAACTGGAACACCGTATGTAGTGTATTTTTCCTCCATTTCTAATAACACCCACCGTTTTATTGAAAAATTGGGGTTTGAACACACCCGCATTCCGGTTAATTTAGATGAACAGATTGAAGTAAACCAGGAGTATGTGCTCTTTTGCCCCACTTACAGTGGTGGTGGGGAATACACATCGGGGGCGGTACCCAAACAGGTAATCCACTTTTTAAACAACAAGCACAACCGTGATTTGTGTCGTGGCGTAATTTCGTCCGGGAACACTAATTTCGGCAATACTTTTGCCATTGCCGGGCCGATTTTGTCGAAAAAGTTAAACGTACCACTTTTATACCAGTTTGAACTTTTAGGCACGAAAAACGATGTAGAACAAGTTCAAACCATTATTACCAATTTCTTTGGTAAAGCCAAATAA", "aa": "MHKDIKIVDASAIVKPTGTPYVVYFSSISNNTHRFIEKLGFEHTRIPVNLDEQIEVNQEYVLFCPTYSGGGEYTSGAVPKQVIHFLNNKHNRDLCRGVISSGNTNFGNTFAIAGPILSKKLNVPLLYQFELLGTKNDVEQVQTIITNFFGKAK", "aa_hexdigest": "8416fb583e6ec22566c1c84e88a63f1d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD652A", "ncbi_nrp_id": "WP_014325461.1", "uniref100_id": "UniRef100_A0AAV5NAT1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD652A", "RefSeq:WP_014325461.1", "UniRef:UniRef100_A0AAV5NAT1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NAT1", "uniref90_id": "UniRef90_P75460" }, "psc": { "uniref90_id": "UniRef90_P75460", "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "uniref50_id": "UniRef50_P47472", "cog_id": "COG1780", "cog_category": "F", "kegg_orthology_id": "K03647", "go_ids": [ "GO:0009263", "GO:0010181", "GO:0036211" ] }, "pscc": { "uniref50_id": "UniRef50_P47472", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47472" ], "product": "Protein NrdI" }, "genes": [ "nrdI" ], "id": "CNOCLNGIAL_712", "locus": "GCA000143945_00686" }, { "type": "cds", "sequence": "contig_1", "start": 381847, "stop": 383475, "strand": "+", "frame": 1, "gene": null, "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "nt": "ATGTCAGTTAAAGAAAAGATTCCTGCTTTCAACACCCAAGAAGACTTAGAAAGCTATATTTCCTTAAATGCCTACACTAAGGTGTACGGTGATTTTAAGATGGATTTACACGCTGTGGAGGCTTACATCCAAGAGCATGTCAAACCCAAAACAAAGGTGTTTCACTCCACCAAGGAACGCTTAGACTTTCTAGTAAAGAATGATTATTACGATGAGAACATCATCAACATGTACAGCTTTGAGCAGTTTGAGGAAATTACACGTAAGGCTTACGCTTACCGTTTTCGTTATGCTAACTTTATGGGGGCATTTAAGTTTTACAACGCTTATGCACTGAAAACCTTTGATGGGAAGTGGTATTTAGAAAACTATGAAGACCGCGTTGTCATGAACGTGCTCTTTTTAGCCAATGGTAACTATAACAAGGCTTTAAAGTTACTCAAACAAATTATTACCAACCGCTTTCAACCAGCTACCCCAACTTTTTTAAATGCGGGACGAAAAAAACGTGGTGAGTTTGTGTCGTGTTACTTACTGCGCATAGAAGACAACATGGAATCAATTGGTCGTGCCATTACCACTACCCTACAGCTCTCCAAGCGTGATGGTGGGGTGGCGTTATTGCTCACTAACATCCGCGAATCGGGTGCGCCAATTAAAAAAATAGAAAACCAGTCATCAGACATTATTCCAATTATGAAGCTGTTGGAAGACTCTTTTTCTTACGCCAACCAATTAGGGCAAAGACAGGGAGCAGGAGCAGTGTATCTCCATGCCCACCACCCCGATGTCATGCAGTTTTTAGACACAAAGCGCGAAAACGCTGATGAAAAGATCCGGATTAAGTCGCTGTCTTTGGGTCTAGTTATTCCCGACATTACCTTTACTTTAGCTAAAAACAATGAGGAGATGGCCTTGTTTAGTCCGTATGATGTGTATGAGGAGTATGGCAAACCCTTGTCAGACATTTCAGTCACGGAAATGTATTACGAACTGTTAGCGAACCAACGCATTAAAAAGACCTTTATTAATGCGCGCAAGTTCTTCCAAACAGTGGCGGAACTGCACTTTGAAAGCGGTTATCCTTACATCTTATTTGACGATACGGTTAACCGGCGCAACGCGCACCCCAACCGCATTGTCATGTCTAACCTCTGTAGTGAAATTGTCCAACCCTCCACTCCATCGGAGTTCCACCACGATTTGGCTTTTAAAAAGGTTGGTAATGACATTAGCTGTAACTTGGGTAGCTTAAACATTGCCAAAGCAATGGAAAGCGGACCGGAGTTCTCGGAGTTGGTTAAACTAGCGATCGAATCACTTGATTTAGTTTCAAGGGTAAGCAATTTAGAAACGGCTCCATCGATCCAAAAGGGTAATTCCGAAAATCATGCTTTGGGATTAGGGGCAATGAACTTGCACGGTTTTTTAGCAACTAACCAAATTTATTACAACTCCCCTGAAGCAATCGACTTTACTAACATCTTTTTCTATACGGTAGCTTACCATGCCTTTAAAGCATCCAGTGAGCTAGCTTTAGAGAAGGGTAAGTTTAAAAACTTTGAAAACACCAAGTTTGCTGATGGGAGCTACTTTGATAAATATATCAAAGTAGAACCAGACTTTTGA", "aa": "MSVKEKIPAFNTQEDLESYISLNAYTKVYGDFKMDLHAVEAYIQEHVKPKTKVFHSTKERLDFLVKNDYYDENIINMYSFEQFEEITRKAYAYRFRYANFMGAFKFYNAYALKTFDGKWYLENYEDRVVMNVLFLANGNYNKALKLLKQIITNRFQPATPTFLNAGRKKRGEFVSCYLLRIEDNMESIGRAITTTLQLSKRDGGVALLLTNIRESGAPIKKIENQSSDIIPIMKLLEDSFSYANQLGQRQGAGAVYLHAHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFTLAKNNEEMALFSPYDVYEEYGKPLSDISVTEMYYELLANQRIKKTFINARKFFQTVAELHFESGYPYILFDDTVNRRNAHPNRIVMSNLCSEIVQPSTPSEFHHDLAFKKVGNDISCNLGSLNIAKAMESGPEFSELVKLAIESLDLVSRVSNLETAPSIQKGNSENHALGLGAMNLHGFLATNQIYYNSPEAIDFTNIFFYTVAYHAFKASSELALEKGKFKNFENTKFADGSYFDKYIKVEPDF", "aa_hexdigest": "93e1d2033388bdfef280c94383d82729", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001C049A88", "query_cov": 0.9022140221402214, "subject_cov": 0.9683168316831683, "identity": 0.679, "score": 679.0, "evalue": 6.39e-241, "valid": false, "product": "ribonucleoside-diphosphate reductase subunit alpha", "ec_ids": [ "1.17.4.1" ], "uniref50_id": "UniRef50_UPI0021AFF10F", "go_ids": [ "GO:0004748", "GO:0005971", "GO:0009265" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021AFF10F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha" }, "genes": [], "id": "CNOCLNGIAL_713", "locus": "GCA000143945_00687" }, { "type": "cds", "sequence": "contig_1", "start": 383581, "stop": 384012, "strand": "+", "frame": 1, "gene": null, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "nt": "TTGGCCAATTCCCACTTACTGGCAATTGCTCCAACGGGTTCAATTTCCTATCTCTCCTCCTGTACTCCTTCACTCCAACCGGTAGTATCCCCGGTAGAAGTGCGCAAGGAAGGTCGGTTGGGACGGATTTATGTACCCGCTTACCAACTCAATAAAGACAGTTACCCTTTTTATAAAGATGGTGCTTACGAACTGGGTCCCGAACCAATTATTAACATAGCAGCAGCGGCACAACAACACGTCGATCAAGCGATTTCGCTCACCCTATTTATGACTGATAAGGCTACCACTCGTGACCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTTCTTCCATTTACTATGTCCGAGTGCGCCAGGAAGTACTAGAGGACAGTGAGGATCACACCATTCAAATGCAGCAGTGTGAGGCCTGTGTGATTTAA", "aa": "MANSHLLAIAPTGSISYLSSCTPSLQPVVSPVEVRKEGRLGRIYVPAYQLNKDSYPFYKDGAYELGPEPIINIAAAAQQHVDQAISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQEVLEDSEDHTIQMQQCEACVI", "aa_hexdigest": "fb6271ea63a8efc0623df6849ecd1699", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A655JDN6", "query_cov": 0.986013986013986, "subject_cov": 0.9929577464788732, "identity": 0.518, "score": 147.0, "evalue": 4.24e-43, "valid": false, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "uniref50_id": "UniRef50_A0A655JDN6" }, "pscc": { "uniref50_id": "UniRef50_A0A655JDN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "product": "Ribonucleotide-diphosphate reductase subunit alpha" }, "genes": [], "id": "CNOCLNGIAL_714", "locus": "GCA000143945_00688" }, { "type": "cds", "sequence": "contig_1", "start": 384159, "stop": 384461, "strand": "+", "frame": 3, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02888", "RefSeq:WP_010874681.1", "SO:0001217", "UniParc:UPI0000133D48", "UniRef:UniRef100_P78026", "UniRef:UniRef50_P47474", "UniRef:UniRef90_P78026" ], "nt": "ATGCATGCTATTGTAGTCTGTGGATCAAAGCAATATTTAGTCCACGAAAATGACACTTTTTTTGTTGAAAAACTTGAAGCGCCAGTTGGTAAAGAAATCCAACTCGACAAGGTTTTAATGTTGGATGAAAAGATTGGTGCGCCATACTTGGAAAAAGCGCGAGTAGTGTGTGTGGTGGAAAAGCACGGTTTACAACGCAAAGTAAACGTGATTAAACACATCTCACAAAAACACCACTTAAAAAAGTATGGTCACCGTCAACCTTACACTAAGTTAAAGGTAGTGCGCTTTGTCCATGATTAA", "aa": "MHAIVVCGSKQYLVHENDTFFVEKLEAPVGKEIQLDKVLMLDEKIGAPYLEKARVVCVVEKHGLQRKVNVIKHISQKHHLKKYGHRQPYTKLKVVRFVHD", "aa_hexdigest": "f4fd694f151cc62406782937221bdfff", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D48", "ncbi_nrp_id": "WP_010874681.1", "uniref100_id": "UniRef100_P78026", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D48", "RefSeq:WP_010874681.1", "UniRef:UniRef100_P78026" ] }, "ips": { "uniref100_id": "UniRef100_P78026", "uniref90_id": "UniRef90_P78026" }, "psc": { "uniref90_id": "UniRef90_P78026", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_P47474", "cog_id": "COG0261", "cog_category": "J", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47474", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47474" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "CNOCLNGIAL_715", "locus": "GCA000143945_00689" }, { "type": "cds", "sequence": "contig_1", "start": 384454, "stop": 384756, "strand": "+", "frame": 1, "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "db_xrefs": [ "COG:COG2868", "COG:S", "EC:3.4.22.-", "GO:0006508", "GO:0008234", "GO:0042254", "KEGG:K07584", "RefSeq:WP_017532771.1", "SO:0001217", "UniParc:UPI00035C8F39", "UniRef:UniRef100_A0AAX0SPC3", "UniRef:UniRef50_P75459", "UniRef:UniRef90_P75459" ], "nt": "ATGATTAAACTAACTGTGTCGCACCACAAACTCGCCGCTTCCGGGCACGCTTTATTTGCCAAAAAGGGGCAAGACATTGTGTGCGCTGCTGTTAGTGGTATTATCTTTGGCGCTTTGCCCTGGTTTGAAACCAACAGCATTGCCGTTCAAGAAGATGCTACTGTACCCAGTTTAAGCTTGGAGTTAGTGCAACCAACCGCTAAGCTTATCACAGGATTGAGTGTGGTGATTATGCAACTTAAAACCCTCGCGCATTCTTACCCGCAGTTTATTTCGTTTGAAGATCAACGTAAAGATGAATAA", "aa": "MIKLTVSHHKLAASGHALFAKKGQDIVCAAVSGIIFGALPWFETNSIAVQEDATVPSLSLELVQPTAKLITGLSVVIMQLKTLAHSYPQFISFEDQRKDE", "aa_hexdigest": "ade646e1f469a9e75d4635aa42d812d9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00035C8F39", "ncbi_nrp_id": "WP_017532771.1", "uniref100_id": "UniRef100_A0AAX0SPC3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00035C8F39", "RefSeq:WP_017532771.1", "UniRef:UniRef100_A0AAX0SPC3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SPC3", "uniref90_id": "UniRef90_P75459" }, "psc": { "uniref90_id": "UniRef90_P75459", "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "ec_ids": [ "3.4.22.-" ], "uniref50_id": "UniRef50_P75459", "cog_id": "COG2868", "cog_category": "S", "kegg_orthology_id": "K07584", "go_ids": [ "GO:0008234", "GO:0006508", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75459", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75459" ], "product": "Ribosomal processing cysteine protease Prp" }, "genes": [ "prp" ], "id": "CNOCLNGIAL_716", "locus": "GCA000143945_00690" }, { "type": "cds", "sequence": "contig_1", "start": 384749, "stop": 385063, "strand": "+", "frame": 2, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "SO:0001217", "UniParc:UPI0001DD652D", "UniRef:UniRef100_A0AAX0SQQ5", "UniRef:UniRef50_P05657", "UniRef:UniRef90_P75458" ], "nt": "ATGAATAACAAATATTTTTTAACCAAAATTGACTTACAGTTTTTTGCTTCCAAAAAGGGAGTTGGTTCAACCAAGAACGGCCGTGATTCTCATGCTAAGCGTTTAGGTGCTAAGAAGGCTGATGGTCAGATGATCCGTACTGGTCAAATTATTTACCGCCAACGCGGTACTAGGGTATGTCCCGGTGTAAATGTTGGCCTTGGTAGTGATGACACCTTGTTTGCTTTAAGCGACGGCTTGGTAAAATACCAAAAGTTTGGACCTAAACAGGGCAAGACCCGCGTGAGTGTGGTCAAACACAAACTAGATGCCTAA", "aa": "MNNKYFLTKIDLQFFASKKGVGSTKNGRDSHAKRLGAKKADGQMIRTGQIIYRQRGTRVCPGVNVGLGSDDTLFALSDGLVKYQKFGPKQGKTRVSVVKHKLDA", "aa_hexdigest": "a847ff3e5cabef129e95365463dcb5c7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD652D", "uniref100_id": "UniRef100_A0AAX0SQQ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD652D", "UniRef:UniRef100_A0AAX0SQQ5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQQ5", "uniref90_id": "UniRef90_P75458" }, "psc": { "uniref90_id": "UniRef90_P75458", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_P05657", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P05657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05657" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [ "rpmA" ], "id": "CNOCLNGIAL_717", "locus": "GCA000143945_00691" }, { "type": "cds", "sequence": "contig_1", "start": 385056, "stop": 385901, "strand": "+", "frame": 3, "gene": "nfo", "product": "putative endonuclease 4", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0003906", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833", "SO:0001217", "UniRef:UniRef50_P47477", "UniRef:UniRef90_P75457" ], "nt": "ATGCCTAAACTCCTAGGAAGCTTTATTTCGTTTAAATCACCCCATTATCTAGTGGGGAGTGTTCGTGACGCTGTTAGCATTAAAGCACAAGCGTTCATGATTTTTTTGGGCGCACCACACACGGCACTACGAGTCGATCCTAATCGTATGCAAATTGACGAGGGTCACACATTGATGGAACAACATAACCTCAGTAAGTCTGGGATGGTTGTCCATGCTCCTTACATTATTAACTGTGCCTCCAAAGATCCGGTAAAGCAAACCTTTGCGATTGACGTTCTGACAAGGGAGGTTAAACTGTGTCATGCCGTGGGTGCTAAACTAATCGTTCTCCACCCCGGTTCAGCGGTTGAGCAAACTCAAACCCAAGCGTTAGATCACCTAATCAAGGTCTTAAATACAGTGATTGCCAACACTAAAGAAGTCATTATTTGTCTCGAAACGATGGCTGGTAAAGGTAATGAAATTGGCCGTGATTTAGACCAGTTAAAGTATGTGATTAACCACATTGAACAACAAGAGCGGATTGGTGTTTGTCTAGATACCTGTCACTTCCATGACAGTGGTAATGACTTTAACAATACTGCCGAAATTATGGAAACGATTGACACTAAACTAGGCTTTGAGTTTTTAAAGGTCATCCACTTAAACGAGTCCAAAAACGTTTGTGGTTCCAAAAAAGACCGTCATGCCAATCTAGGTGAGGGGATGATTGGGTTTGATAATTTAATGCGCTTTATTGCCCAACCGCAAATAAAGCAAATACCGATTGTTTTAGAAACCCCATCTGACAAGCATAATTATCCCGCTGTATATGGTGCTGAAATCGAAAGGATCCGTGCTTGA", "aa": "MPKLLGSFISFKSPHYLVGSVRDAVSIKAQAFMIFLGAPHTALRVDPNRMQIDEGHTLMEQHNLSKSGMVVHAPYIINCASKDPVKQTFAIDVLTREVKLCHAVGAKLIVLHPGSAVEQTQTQALDHLIKVLNTVIANTKEVIICLETMAGKGNEIGRDLDQLKYVINHIEQQERIGVCLDTCHFHDSGNDFNNTAEIMETIDTKLGFEFLKVIHLNESKNVCGSKKDRHANLGEGMIGFDNLMRFIAQPQIKQIPIVLETPSDKHNYPAVYGAEIERIRA", "aa_hexdigest": "62a774368a256e0f18ae38b14afee857", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75457", "query_cov": 1.0, "subject_cov": 0.9825174825174825, "identity": 1.0, "score": 566.0, "evalue": 6e-204, "valid": true, "gene": "nfo", "product": "Probable endonuclease 4", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_P47477", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0008833", "GO:0003677", "GO:0003906", "GO:0008081", "GO:0008270", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_P47477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47477" ], "product": "Probable endonuclease 4" }, "genes": [ "nfo" ], "id": "CNOCLNGIAL_718", "locus": "GCA000143945_00692" }, { "type": "cds", "sequence": "contig_1", "start": 385870, "stop": 386346, "strand": "+", "frame": 1, "gene": "fur", "product": "putative MG236-like protein", "db_xrefs": [ "COG:COG0735", "COG:P", "GO:0000976", "GO:0003700", "GO:0008270", "GO:0045892", "GO:1900376", "RefSeq:WP_010874685.1", "SO:0001217", "UniParc:UPI00001394D1", "UniRef:UniRef100_P75456", "UniRef:UniRef50_P75456", "UniRef:UniRef90_P75456" ], "nt": "ATGGTGCTGAAATCGAAAGGATCCGTGCTTGATTTGGAGCACGATAACCAAACACTAAAGGATTACATTAGTGTGTTTGAACGCAATAAGATGCGTTTAACCCAATCACGCTTAATGTTGCTCCAATGCTTAGTGCAACACCGTGACTGGCACACCTTAGCTGAGCTCAAACACCACTTGGAACAAAACCAACAGCGCACTACCTTAGCTTCTATCTATAACAACCTCAAGATTTTTGCTAAGTTAAAGCTAATTAATATTTTTGTAGATACCAATCGCTTTGAAACGTATTATTGCTTAAGACACGAAAACCACAAGCACATTTACTTCTTTGACGAAAACCAGCGCAAGTTTTTAACCCTACCACTACAAGACAAGGAAGCGTTGAGTTTAATTGGACATAAAAGTAAACATGGCAAAATTAAACTGAATGACTTTTACATAGTCGCTAGCGGTACACTTGAAGATGATCAATAA", "aa": "MVLKSKGSVLDLEHDNQTLKDYISVFERNKMRLTQSRLMLLQCLVQHRDWHTLAELKHHLEQNQQRTTLASIYNNLKIFAKLKLINIFVDTNRFETYYCLRHENHKHIYFFDENQRKFLTLPLQDKEALSLIGHKSKHGKIKLNDFYIVASGTLEDDQ", "aa_hexdigest": "6ae7dda45d01c26158f5d460a185332a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D1", "ncbi_nrp_id": "WP_010874685.1", "uniref100_id": "UniRef100_P75456", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D1", "RefSeq:WP_010874685.1", "UniRef:UniRef100_P75456" ] }, "ips": { "uniref100_id": "UniRef100_P75456", "uniref90_id": "UniRef90_P75456" }, "psc": { "uniref90_id": "UniRef90_P75456", "gene": "Fur", "product": "Uncharacterized protein MG236 homolog", "uniref50_id": "UniRef50_P75456", "cog_id": "COG0735", "cog_category": "P", "go_ids": [ "GO:0003700", "GO:0000976", "GO:0008270", "GO:0045892", "GO:1900376" ] }, "pscc": { "uniref50_id": "UniRef50_P75456", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75456" ], "product": "Uncharacterized protein MG236 homolog" }, "genes": [ "fur" ], "id": "CNOCLNGIAL_719", "locus": "GCA000143945_00693" }, { "type": "cds", "sequence": "contig_1", "start": 386336, "stop": 387220, "strand": "+", "frame": 2, "gene": null, "product": "putative MG237-like protein", "db_xrefs": [ "RefSeq:WP_010874686.1", "SO:0001217", "UniParc:UPI00001394D4", "UniRef:UniRef100_P75455", "UniRef:UniRef50_P75455", "UniRef:UniRef90_P75455" ], "nt": "ATGATCAATAAGCCAAATCAGTTTGTCAACCACTTAAGTGCTTTAAAAAAACACTTTGCCTCTTATAAGGAGCTCCGTGAAGCGTTTAACGATTATCACAAGCATAACGGTGATGAGTTGACCACCTTCTTTTTGCACCAGTTTGATAAGGTGATGGAACTAGTCAAGCAGAAGGACTTTAAAACTGCCCAGTCTCGCTGTGAGGAGGAATTAGCCGCCCCTTATCTACCAAAACCTTTAGTAAGCTTTTTCCAATCGCTACTCCAGCTCGTGAACCATGATTTATTGGAACAGCAAAACGCGGCCTTGGCTAGTTTACCCGCAGCCAAAATCATAGAGCTTGTTTTACAGGATTACCCCAATAAACTCAACATGATTCACTACCTTTTGCCCAAAACGAAGGCGTTTGTCAAACCACATTTGTTACAACGCTTACAGTTTGTGTTAACTGATTCGGAGTTGTTGGAATTAAAGCGCTTTTCCTTCTTTCAAGCACTCAACCAGATCCCGGGCTTTCAGGGTGAGCAAGTTGAGTACTTTAACAGTAAGCTCAAACAAAAGTTTACCTTGACCTTAGGGGAGTTTGAAATTGCCCAACAGCCAGATGCCAAAGCATACTTTGAGCAGTTAATTACCCAAATCCAACAGCTCTTTTTAAAAGAGCCGGTTAACGCTGAGTTTGCCAATGAGATTATTGATGCCTTTTTGGTGAGCTATTTCCCCCTCCATCCCCCAGTACCTTTGGCACAATTAGCTGCCAAGATTTATGAGTATGTCAGCCAAATTGTGCTTAACGAAGCGGTTAACCTAAAGGATGAACTGATCAAACTAATCGTTCATACGCTTTACGAACAGTTAGATCGTCCTGTTGGGGATGAAAATTAA", "aa": "MINKPNQFVNHLSALKKHFASYKELREAFNDYHKHNGDELTTFFLHQFDKVMELVKQKDFKTAQSRCEEELAAPYLPKPLVSFFQSLLQLVNHDLLEQQNAALASLPAAKIIELVLQDYPNKLNMIHYLLPKTKAFVKPHLLQRLQFVLTDSELLELKRFSFFQALNQIPGFQGEQVEYFNSKLKQKFTLTLGEFEIAQQPDAKAYFEQLITQIQQLFLKEPVNAEFANEIIDAFLVSYFPLHPPVPLAQLAAKIYEYVSQIVLNEAVNLKDELIKLIVHTLYEQLDRPVGDEN", "aa_hexdigest": "e70a6b3f75a97b33ae1981c5cba6968a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D4", "ncbi_nrp_id": "WP_010874686.1", "uniref100_id": "UniRef100_P75455", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D4", "RefSeq:WP_010874686.1", "UniRef:UniRef100_P75455" ] }, "ips": { "uniref100_id": "UniRef100_P75455", "uniref90_id": "UniRef90_P75455" }, "psc": { "uniref90_id": "UniRef90_P75455", "product": "Uncharacterized protein MG237 homolog", "uniref50_id": "UniRef50_P75455" }, "pscc": { "uniref50_id": "UniRef50_P75455", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75455" ], "product": "Uncharacterized protein MG237 homolog" }, "genes": [], "id": "CNOCLNGIAL_720", "locus": "GCA000143945_00694" }, { "type": "cds", "sequence": "contig_1", "start": 387368, "stop": 388579, "strand": "+", "frame": 2, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005737", "GO:0005854", "GO:0006457", "GO:0015031", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0140839", "GO:0140840", "SO:0001217", "UniRef:UniRef50_P47480", "UniRef:UniRef90_P75454" ], "nt": "ATGAAAATCAAGGGTTTTCGTAAAGGTAAAGTGCCTCCAACTTTAGCTAAGGATTACTTAGACCGTGCCGAGTTGTTGCAACGTAGCGCCCAAGCGGTAATTGATGCCATCTTCCAGCCGTTACAACAAGAAGCAGTAATTGCTGACAACGAGAATGTAATTGAGGACTTTCCTACCATTGACTTTAAAACAATCAATGAGAACGATTGTGTTATTCTGTTTGACTTTGATCTCGTACCCCAGTTTGAACTACCTGATTACAAGCACATCAAGGATTTAAGCCCGATTGTACCCCTAAAGGATGAGGAGTTCAACAAGGAGTTGCACAACATCGAAAAGAACAAAGGGAAACTAGTTGATGTTAGTGACAAAGCATTGGCCAACAATGATATTGCCGTCATTGACTTTGTTGGCAAGGTAGATGGTAAGGTGCTTGAAAGCGCTACTGCCAAACAATACGAATTAACGATTGGCTCCAACAGCTTTATTGATGGCTTTGAAAGTGGTTTAATTGGCATGAAAGTAGGGGACAAGCGTCAGTTAAAGTTAAAGTTCCCCAAGGACTACCATGCTGAAGAATTGAAGGGTAAACCAGTAGAGTTTGACATAGAGCTCAAAGCAATTAAGCAGTTAGAGATCACCCCCATGGATGAAACTAACTTTAAGGAGTATTTACCCGCACAATACCAGGGTTTCAACTCACTCAAGGAATTTAAAACTTACTTTCATAAGTTAGTTTCCGCTAAGAAACTGGAAATTACCCTACAAGAAAACAGTGTTAAAATTCGCCAGTTCTTTTTAGCTAACACCACCTTACCTTACATTCCTGATTCCTTAATTAAATTGGAATCCGATCGCTTACTGAGAGCGCAAAAGGATCAAGCGGAGCAGTACAAGATTCCCTTTGAGAGATTGTTAGCAGCTTCCAAGCTTTCATTGGAGCAGTTGCAACAACGTAACATAAAAGAAGCACGTGATAACGTTACCTTTGCTTTAGTGATGAAACGAATCGCTGATGTGGAAAAAATCAAGGTTGATAACAAAAAGATTCACAGCGAAATCGAATCGATTATTGATGTTGAATACCCCTTTGTTAATGCCGAGTTGAAAAAACAAATGTTCCACAACATGGAACAACAAAAGGACTTTGTGGAATCGATAATTTTGAACCGTTTAACCACTACCAAGATAATTGAGTACTCCACTCATTAG", "aa": "MKIKGFRKGKVPPTLAKDYLDRAELLQRSAQAVIDAIFQPLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVPQFELPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLVDVSDKALANNDIAVIDFVGKVDGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPVEFDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSVKIRQFFLANTTLPYIPDSLIKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRNIKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIIDVEYPFVNAELKKQMFHNMEQQKDFVESIILNRLTTTKIIEYSTH", "aa_hexdigest": "70eea71f7649d9473dd157ddb7dfcae4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75454", "query_cov": 1.0049627791563276, "subject_cov": 0.9080717488789237, "identity": 0.995, "score": 765.0, "evalue": 5.22e-278, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_P47480", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005737", "GO:0005854", "GO:0006457", "GO:0015031", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0140839", "GO:0140840" ] }, "pscc": { "uniref50_id": "UniRef50_P47480", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47480" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "CNOCLNGIAL_721", "locus": "GCA000143945_00695" }, { "type": "cds", "sequence": "contig_1", "start": 388650, "stop": 389630, "strand": "+", "frame": 3, "gene": null, "product": "LON peptidase substrate-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E", "UniRef:UniRef90_UPI001F21836E" ], "nt": "ATGCCAGCTGTAAAAAAACCACAAATTCTCGTTGTGCGTAACCAAGTTATCTTTCCGTACAACGGCTTTGAACTTGATGTCGGTCGTGAACGCTCGAAAAAACTAATTAAAGCACTCAAAAACCTCAAAACCAAACGTTTGGTGTTAGTCACCCAAAAGAATTCAGATCAGCTCAACCCTGAGTTTGATGACATTTACCATTGCGGTACTTTATGTGACATTGATGAAATTATTGAAGTACCGAGTGAAGATGGCAAGACAGCTGACTATAAGATTAAGGGTAAAGGATTACAGCGCGTTGCCATTACAAGCTTCTCTGATGCTGATTTAACCAAGTACGACCATCACTTTCTCAACTCTACTCTAACAGAAAATAAAGCGTTAGATAAGTTGTTGGAACGGATCTTTCCCGATAAAGAAGACTTTGCTGAAATTTTAGACAGCTTAAACAGCTTCTTGGAACTGCAAGAGTTAAAGAAGCTATCTAAGGTACCAAAAGACATAAAACGGTATGACATTATTACCTTCAAGCTTGCTAGCTTAATTTTCAAAGACATTACCCTCCAACAAGCCATTTTGGAAGAAAATGACATTGAAAAACGGTTACAGAAGATCATTGGTTCTGGCATCGAGGATTTAGGTCACATCAGCGAAGAAGCCCGCGCTAAACAACGCGAAAGTGAGTTTGACAAGATTGACAACCGCATCACGCGCAAAGTTAACGAACAGTTATCGCGCCAACAACGTGACTTTTACCTCCGGGAAAAACTCCGGGTTATCCGTGAAGAGATCGGGATGACTTCCAAGAAGGAAGATGAAGTATCCAACATTCGCAAAAAGCTAGAGGAAAACCCATATCCAGAGCACATTAAAAAACGGATTTTAAGTGAGTTAGATCACTTTGAAAACTCCTCCTCTTCATCACAAGAATCAACCTTAACCAAGACTTACATCGACACTTTGATGAACTTGCCATGGTGA", "aa": "MPAVKKPQILVVRNQVIFPYNGFELDVGRERSKKLIKALKNLKTKRLVLVTQKNSDQLNPEFDDIYHCGTLCDIDEIIEVPSEDGKTADYKIKGKGLQRVAITSFSDADLTKYDHHFLNSTLTENKALDKLLERIFPDKEDFAEILDSLNSFLELQELKKLSKVPKDIKRYDIITFKLASLIFKDITLQQAILEENDIEKRLQKIIGSGIEDLGHISEEARAKQRESEFDKIDNRITRKVNEQLSRQQRDFYLREKLRVIREEIGMTSKKEDEVSNIRKKLEENPYPEHIKKRILSELDHFENSSSSSQESTLTKTYIDTLMNLPW", "aa_hexdigest": "f5dded132c55a2ffa45747c08e59f906", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F21836E", "query_cov": 0.8006134969325154, "subject_cov": 1.0, "identity": 0.985, "score": 490.0, "evalue": 1.52e-173, "valid": true, "product": "LON peptidase substrate-binding domain-containing protein", "uniref50_id": "UniRef50_UPI001F21836E" }, "pscc": { "uniref50_id": "UniRef50_UPI001F21836E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E" ], "product": "LON peptidase substrate-binding domain-containing protein" }, "genes": [], "id": "CNOCLNGIAL_722", "locus": "GCA000143945_00696" }, { "type": "cds", "sequence": "contig_1", "start": 389676, "stop": 391037, "strand": "+", "frame": 3, "gene": "lon", "product": "endopeptidase La", "db_xrefs": [ "COG:COG0466", "COG:O", "EC:3.4.21.53", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565", "SO:0001217", "UniRef:UniRef50_P47481", "UniRef:UniRef90_P78025" ], "nt": "TTGAACAAGAACCACTCAGGACTAGATAAGGTAAAGGAACGAGTGGTTGAGTATTTAGCAGTACAGCTCCGTACCAAAAAGCTTAAGGGTCCGATTATGTGTTTAGTCGGTCCACCTGGAGTTGGTAAATCCAGTTTAGCCAAATCAATTGCTGAAGCGTTAAACAAGCGCTTTGTTAAGGTTTCATTGGGCGGGGTGCATGATGAATCGGAAATCCGTGGTCACCGTAAAACCTATTTGGGTTCGATGCCAGGACGGATTTTAAAGGGCATGGCACGTGCTAAGGTTATTAACCCACTGTTCTTGTTAGATGAAATTGACAAGATGACATCCTCCAATCAAGGTTACCCTTCCGGCGCTTTGTTAGAGGTATTAGATCCGGAGTTAAACAATAAGTTTAGCGATAACTATGTTGAGGAGGATTATGACCTGTCTAAGGTAATGTTTGTAGCCACCGCTAACTATATCGAAGATATTCCGGAAGCCTTGTTAGACCGGATGGAAGTGATTGAACTTACCTCTTACACCGAACAGGAAAAGCTACAAATTACAAAGAGCCACTTAGTTAAGCGTTGTTTAGATGACGCTGAGATTAAAACAGATGACCTCAAGTTTACCGATGAGGGTATTAGCTATATCATTAAGTTTTACACGAGGGAAGCGGGGGTGAGACAGTTAGAACGCTTAATCCAGCAAATTGTGCGTAAGTACATTGTTAACTTGCAAAAAACCGGCGAACAACAGGTAGTAGTTGATGTAGATTTGGTAAAGAAATACCTCAAGAAAGAAATCTTTGACTATACCGTGCGCGATGAAGATGCACTTCCGGGAATTGTTAACGGCATGGCTTACACCCCAACTGGTGGTGATCTGTTACCAATTGAAGTAACCCATGTAGCTGGTAAGGGTGATTTAATTCTGACCGGCAATTTAAAGCAAACAATGCGTGAAAGTGCTAGCGTAGCGTTAGGTTATGTCAAGGCCAATGCGCAGAGCTTTAACATTAACCCTAACCTCTTTAAAAAGGTTGACATTAACATCCATGTTCCAGGTGGTGGTATTCCCAAGGATGGCCCGAGTGCTGGCGCGGCTTTAGTTACTGCCATTATCTCGTCTTTAACTGGCAAAAAGGTTGACCCTAAGATTGCCATGACCGGGGAGATTACCCTAAGGGGTAAAGTAATGACCATAGGCGGGGTGAAAGAAAAAACCATTTCCGCCTACCGCGGTGGAGTACGCACCATCTTTATGCCCGAAAAGAATGAGCGTTACTTAGATGAAGTGCCTAAAGACATCGTTAAGGACTTGGAGATTATCCTAGTAAAGGAATACAAGGACATCTACAACAAGATATTTAATTAA", "aa": "MNKNHSGLDKVKERVVEYLAVQLRTKKLKGPIMCLVGPPGVGKSSLAKSIAEALNKRFVKVSLGGVHDESEIRGHRKTYLGSMPGRILKGMARAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFVATANYIEDIPEALLDRMEVIELTSYTEQEKLQITKSHLVKRCLDDAEIKTDDLKFTDEGISYIIKFYTREAGVRQLERLIQQIVRKYIVNLQKTGEQQVVVDVDLVKKYLKKEIFDYTVRDEDALPGIVNGMAYTPTGGDLLPIEVTHVAGKGDLILTGNLKQTMRESASVALGYVKANAQSFNINPNLFKKVDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPKIAMTGEITLRGKVMTIGGVKEKTISAYRGGVRTIFMPEKNERYLDEVPKDIVKDLEIILVKEYKDIYNKIFN", "aa_hexdigest": "c5b33583c7d31917b30b13905c76d26b", "start_type": "TTG", "rbs_motif": "AAAAA", "psc": { "uniref90_id": "UniRef90_UPI0025901479", "query_cov": 0.9867549668874173, "subject_cov": 0.8799212598425197, "identity": 0.523, "score": 493.0, "evalue": 7.23e-169, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A7C6Y0Z4", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "genes": [ "lon" ], "truncated": "5-prime", "pseudogene": { "start": 389376, "stop": 391037, "observations": { "mutation": [ 389628 ], "stop": [ 389628 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78025", "query_cov": 1.0, "subject_cov": 0.5490909090909091, "identity": 0.998, "score": 879.0, "evalue": 2.53e-316, "gene_start": 1, "gene_end": 453, "reference_start": 373, "reference_end": 825, "reference_sequence": "MSTPLISTQSIRVLKNVLQFSILQPLILITMPAVKKPQILVVRNQVIFPYNGFELDVGRERSKKLIKALKNLKTKRLVLVTQKNSDQLNPEFDDIYHCGTLCDIDEIIEVPSEDGKTADYKIKGKGLQRVAITSFSDADLTKYDHHFLNSTLTENKALDKLLERIFPDKEDFAEILDSLNSFLELQELKKLSKVPKDIKRYDIITFKLASLIFKDITLQQAILEENDIEKRLQKIIGSGIEDLGHISEEARAKQRESEFDKIDNRITRKVNEQLSRQQRDFYLREKLRVIREEIGMTSKKEDEVSNIRKKLEENPYPEHIKKRILSELDHFENSSSSSQESTLTKTYIDTLMNLPWWQESKDNADVKNLIKILNKNHSGLDKVKERVVEYLAVQLRTKKLKGPIMCLVGPPGVGKSSLAKSIAEALNKRFVKVSLGGVHDESEIRGHRKTYLGSMPGRILKGMARAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFVATANYIEDIPEALLDRMEVIELTSYTEQEKLQITKSHLVKRCLDDAEIKTDDLKFTDEGISYIIKFYTREAGVRQLERLIQQIVRKYIVNLQKTGEQQVVVDVDLVKKYLKKEIFDYTVRDEDALPGIVNGMAYTPTGGDLLPIEVTHVAGKGDLILTGNLKQTMRESASVALGYVKANAQSFNINPNLFKKVDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPKIAMTGEITLRGKVMTIGGVKEKTISAYRGGVRTIFMPEKNERYLDEVPKDIVKDLEIILVKEYKDIYNKIFN" }, "paralog": false, "identity": 0.9981916817359855, "score": 1078.0, "evalue": 0.0, "description": "Internal stop codon at 389628. Nonsense mutation around 389628.", "psc": { "uniref90_id": "UniRef90_P78025", "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_P47481", "cog_id": "COG0466", "cog_category": "O", "go_ids": [ "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78025", "EC:3.4.21.53", "UniRef:UniRef50_P47481", "COG:COG0466", "COG:O", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ] }, "pscc": { "uniref50_id": "UniRef50_P47481", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47481" ], "product": "Lon protease" } }, "id": "CNOCLNGIAL_723", "locus": "GCA000143945_00697" }, { "type": "cds", "sequence": "contig_1", "start": 391349, "stop": 391888, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12679.13" ], "nt": "GTGACACTGTTAGCGGGCAGTATTACCGCTGACATCTTTATTGAAGGGTACAACAACGGGATTGAGCTGTTCTTGTTCTCTAAACCTATTAGCCGTGACCGCATTTTGCTCATTAAGTTACTAGTGCTGTTAATGTACTTGAGTATTGTAGCCGTACTATCCACTGCTGTAGTGAGCTTGGGTTACTTTACGCGCAATGAGATTGAGAGTTATTTAGATGATTTAGCTTTAAGTATCTTTGTCGGTACCATTTTAAACGGCATTATCTTTAGCGCTGTAACTATTATGTTAGCCACGCGCTTTAGCAACATCCAGAGCTTAATCATTTTGCTCTTGTTTGTGTCGTTGTTTAGCTTTTCCTCACCAATTGCGCGGTTAATCTTCGACACGCCTGCTAAAATGTTGAGCCAACAAGGTTACAGCGTTCGCCAAATTAACGGAATTGCGCACAGCGAAAGCCCTAATGTAGAGCGTAAAATTTACGTTTCGGTCAAAAAGCGGCGTTTAACTGGGGTGGATCGCGAAGAAAAAGAGCTTTGA", "aa": "MTLLAGSITADIFIEGYNNGIELFLFSKPISRDRILLIKLLVLLMYLSIVAVLSTAVVSLGYFTRNEIESYLDDLALSIFVGTILNGIIFSAVTIMLATRFSNIQSLIILLLFVSLFSFSSPIARLIFDTPAKMLSQQGYSVRQINGIAHSESPNVERKIYVSVKKRRLTGVDREEKEL", "aa_hexdigest": "20febea09cbc846dfa573b01ed52a144", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12679.13", "name": "ABC2_membrane_2", "length": 128, "aa_cov": 0.7039106145251397, "hmm_cov": 0.9921875, "evalue": 1.2698644698980819e-09, "score": 33.192134857177734, "start": 3, "stop": 128 } ], "seq_stats": { "molecular_weight": 19998.346099999995, "isoelectric_point": 8.92314968109131 }, "id": "CNOCLNGIAL_724", "locus": "GCA000143945_00698" }, { "type": "cds", "sequence": "contig_1", "start": 391925, "stop": 392332, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGAGGAGGCACAACGAACCTCCTCTTACATTAATTCCTTTTACACCAACATTGGTTACTTATTTGGCTCGCTTTACCGCTTGGGTGCGTTAGCTGATGAAAATAACTTTGATGCATCAGTAGCAACCCAAAACACCAAAATTCGCTTTGGTAAGTTAGTTGATTTAGATAAGAGTACTGACATTATTGGTTATCGCACTAAAGACCGCTTTGGCCGTTTCTTTCGCTTTCACTTGCGCTTTCCAACGCTCAACTTTAAGCAAAGCTTGAGTAACAGCGGCGTTTTCTTTATCAATAAACGGGGATTAGTTAAAGCTACTTTTAGTGCTGGCGATGATGCCTTTAAACGTGACCCGAAGATAATGCAAAAGGTGTTTGACACTTTTCTAGAAAGTTTTAGTTGA", "aa": "MYEEAQRTSSYINSFYTNIGYLFGSLYRLGALADENNFDASVATQNTKIRFGKLVDLDKSTDIIGYRTKDRFGRFFRFHLRFPTLNFKQSLSNSGVFFINKRGLVKATFSAGDDAFKRDPKIMQKVFDTFLESFS", "aa_hexdigest": "ecd16d1d22dc9eb0d09bd42a00d2966c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15598.45779999998, "isoelectric_point": 9.652222633361816 }, "id": "CNOCLNGIAL_725", "locus": "GCA000143945_00699" }, { "type": "cds", "sequence": "contig_1", "start": 392522, "stop": 392629, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAGTGAAAGCACTGCCACCGGTATTTCTAGTGATCCCCAAAGTCAGGGCAAAATGAACAACAAAAGTGAGGAACAAAAAAAGAAAGAAAAAGAACTCACTTGA", "aa": "MSSESTATGISSDPQSQGKMNNKSEEQKKKEKELT", "aa_hexdigest": "0601b64c4b95ea4f51e647d93046f2b4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3844.1550000000007, "isoelectric_point": 6.117035102844238 }, "id": "CNOCLNGIAL_726", "locus": "GCA000143945_00700" }, { "type": "cds", "sequence": "contig_1", "start": 392693, "stop": 393259, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGGGACAATGTAGCTTTTTTAAATAAAGTGAACGCTGAAGACCAAAACGGGGATAAAAACCAGTTTGCCCAAGCAATGGCATCACTCAATAAGCAAATTAACATGTTTTACGTGTTGAATGAAATTACCAACAAGGAACACAATTTGCCAACTAAATACGGTGCTTATTCTGATCAATTAAAAAAGGTCATAAACGAAAACTTTGAAAAGATAGAAAATAAGAAAAAGGAGATAGAGGAAAAACAAAATAAAGAATTTCAGGAAAACAAGAATGGTGCATCAAATAATCAAGACACAAAGCAAGACGCACAAAAAGGTGATACCAATACCCAAAGCACTGAGTTAAAAGCTCGTCAAGCACAACAAGTGCAAAAGGATCAACAAAATGACAGTAAAGGCAATACTGCTACAAACAGTGATACTGGTAAATCGGACAATAAAACCGATACCACTGAAGATGAAAAAAACACATATAAACCCTTAACAGCTAGGGAGAAAAAACCAAAATTAAGGAGCTTACTAAGGACATCTTCTTCTCTACAGCGCAGAACTATTTGTTCTTAG", "aa": "MWDNVAFLNKVNAEDQNGDKNQFAQAMASLNKQINMFYVLNEITNKEHNLPTKYGAYSDQLKKVINENFEKIENKKKEIEEKQNKEFQENKNGASNNQDTKQDAQKGDTNTQSTELKARQAQQVQKDQQNDSKGNTATNSDTGKSDNKTDTTEDEKNTYKPLTAREKKPKLRSLLRTSSSLQRRTICS", "aa_hexdigest": "89549a95757253bc1c58be8e7047643b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21542.397800000006, "isoelectric_point": 8.96382923126221 }, "id": "CNOCLNGIAL_727", "locus": "GCA000143945_00701" }, { "type": "cds", "sequence": "contig_1", "start": 393259, "stop": 393483, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000AF58EEB", "UniRef:UniRef100_A0AB38W6Z6", "UniRef:UniRef50_A0AB38W6Z6", "UniRef:UniRef90_A0AB38W6Z6" ], "nt": "GTGCAAGGTGGGTATAACAACAAGTTGTTTGCTGGTGGTTTACCAGAGCTCCACCAACGCTTCTTGTCAGACGTAGTGGTTGATTTCTCTGAGGAAAAACTCGTTTATGAAGTACAAGGTGAACCGATTGTATCCAGTGTAGCCACCATTGTGATTACCCTATTTTTAACAGTTGTTTTATTAGCTATTGCCTTTTTCTTCTTTAAATACCGCAACGTTAAATAG", "aa": "MQGGYNNKLFAGGLPELHQRFLSDVVVDFSEEKLVYEVQGEPIVSSVATIVITLFLTVVLLAIAFFFFKYRNVK", "aa_hexdigest": "677be3f9032ef713cdab53ea93b949db", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000AF58EEB", "uniref100_id": "UniRef100_A0AB38W6Z6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000AF58EEB", "UniRef:UniRef100_A0AB38W6Z6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6Z6", "uniref90_id": "UniRef90_A0AB38W6Z6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6Z6", "uniref50_id": "UniRef50_A0AB38W6Z6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6Z6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6Z6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8357.670599999994, "isoelectric_point": 5.64407787322998 }, "id": "CNOCLNGIAL_728", "locus": "GCA000143945_00702" }, { "type": "cds", "sequence": "contig_1", "start": 393582, "stop": 393851, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCCCGGTGAGTTCCATGCCTTTATTGGTCAAAATGGTGCGGGGAAAACTACCACCATTAAGTGTTTAATTTCATCTTATCAGCGCTTTAAGGACGAAATTAACATCGATGGCATTAGCAATAAAAACGCCAAGTCTAAGGGTGTAATTAGTTATATCCCTGAATATGCTGTGTTTCCCAAACACCTCAATACCCATGAATACCTCTACACGTTGGGTAAACTGTCAGGTTGTAGTTTGCAAACCATTAAGGAAAAGGTTGATTACTGA", "aa": "MPGEFHAFIGQNGAGKTTTIKCLISSYQRFKDEINIDGISNKNAKSKGVISYIPEYAVFPKHLNTHEYLYTLGKLSGCSLQTIKEKVDY", "aa_hexdigest": "e43cb07d3092f0b783e035908a936b46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 69, "aa_cov": 0.7415730337078652, "hmm_cov": 1.0, "evalue": 2.411636872193291e-11, "score": 39.8638801574707, "start": 2, "stop": 67 } ], "seq_stats": { "molecular_weight": 9961.279999999993, "isoelectric_point": 8.887047386169435 }, "id": "CNOCLNGIAL_729", "locus": "GCA000143945_00703" }, { "type": "cds", "sequence": "contig_1", "start": 393969, "stop": 394223, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAGCCCACTGCTAACTTAGATCCCAAAACACGTAATGAGTTTATGGATGTGTGTTACGAACTTAATGTACGTAACAAGATGGCGGTGTTTGTTTCATCCCATATTTTGGCTGAATTGGAAAACTATTGTGATTCGCTCACTGTTATTCATGAAGGGCAAATTCTTTTTAATGGCAAAACAAAGAACATTGCCAAAGACAACTTTGGTTACCGTTTAAAGGTTAACCATTCCGATAGTTTACGTAAGTGA", "aa": "MDEPTANLDPKTRNEFMDVCYELNVRNKMAVFVSSHILAELENYCDSLTVIHEGQILFNGKTKNIAKDNFGYRLKVNHSDSLRK", "aa_hexdigest": "1d12abd41efc0d6efdb48e53de64b691", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9691.906899999987, "isoelectric_point": 6.261519813537597 }, "id": "CNOCLNGIAL_730", "locus": "GCA000143945_00704" }, { "type": "cds", "sequence": "contig_1", "start": 394669, "stop": 395310, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912123E", "UniRef:UniRef90_UPI001912123E" ], "nt": "ATGTCGAGTTTAATCATTCCTTTCATTGCTCTGTTTGCCACCATTATTACCTTTCAAGTCTTTATTAATGGTTATTACAACGGTCTAGAGATCTTATTAATTACCCGCTTTTTTACTCGCGGTCGGTTATTCTTTGCTCGCTTAGCAGTCTTGTTTATTTGGATTACTGGCACGGCATTTTTAAGTGGTCTTTTTGTTAGTTTAACTGCTACTTTAGGTGCTACATCACAAACAGTTACTGACCTTGTTTTAAGCGTCTTTTTTGGAGTGCTTTTACTTAGTTTATTGTTTAGCTGTGTCTTGATTATTATCGCGCAGTTTTTAAACCGCACCCAAAGTATGTTGTTGTTACTGTTAGGGGTTTCCCTAGCTAGCTTTACAACCGTTATCTTGGCCTTTTCAATTAAGCCACCGAGTGAAAATCTCCGTGATAATAGTTACCAACCACTAAACCTAAGTCTCATCAGTAAGTCTAAGTCCAAGGGTGTCGAAAATGTCTTTACCATCATTGACTCTTCAAATGTATCCGATCCGCGCAATAAAAAGAAATCGATTGTAAAAACCAATCCGCAAGCGATTTGGGATGAATATGGGGAGAGTAGCTTCTTTCAAAGCCAGTATTACTGGAATGTAGGGTATTGA", "aa": "MSSLIIPFIALFATIITFQVFINGYYNGLEILLITRFFTRGRLFFARLAVLFIWITGTAFLSGLFVSLTATLGATSQTVTDLVLSVFFGVLLLSLLFSCVLIIIAQFLNRTQSMLLLLLGVSLASFTTVILAFSIKPPSENLRDNSYQPLNLSLISKSKSKGVENVFTIIDSSNVSDPRNKKKSIVKTNPQAIWDEYGESSFFQSQYYWNVGY", "aa_hexdigest": "1552749d2db6258031c2ea01960eba80", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 394453, "stop": 395613, "observations": { "mutation": [ 394471, 394552, 394561, 394648, 395308 ], "stop": [ 394471, 394552, 394561, 394648, 395308 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001912123E", "query_cov": 1.0, "subject_cov": 0.49882903981264637, "identity": 1.0, "score": 384.0, "evalue": 3.89e-131, "gene_start": 1, "gene_end": 213, "reference_start": 73, "reference_end": 285, "reference_sequence": "MKLFKNWSDVNNVKQRQLLLAYFRFILKQTVKSWLLWVTAGLVLFLLALVLLIIPAFTKQDPLFLWSHPVVQMSSLIIPFIALFATIITFQVFINGYYNGLEILLITRFFTRGRLFFARLAVLFIWITGTAFLSGLFVSLTATLGATSQTVTDLVLSVFFGVLLLSLLFSCVLIIIAQFLNRTQSMLLLLLGVSLASFTTVILAFSIKPPSENLRDNSYQPLNLSLISKSKSKGVENVFTIIDSSNVSDPRNKKKSIVKTNPQAIWDEYGESSFFQSQYYWNVGYWINSLFRLNSLSDYRNFDVNLYFLNTKLNFDREVDTDKMVDFVSFRDQRHLYFLSYSFFINTHNLPTQPLPRILTYDNNGAFLEKIVPSFDDIKLDPPRVKKVYKFFSDTIIQSFRDYESEEKERQEKEEKEKAEKDNGNGQ" }, "paralog": false, "identity": 0.9870466321243523, "score": 734.0, "evalue": 1.49e-271, "description": "Internal stop codon at 394471, 394552, 394561, 394648, 395308. Nonsense mutation around 394471, 394552, 394561, 394648, 395308.", "psc": { "uniref90_id": "UniRef90_UPI001912123E", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI001912123E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001912123E", "UniRef:UniRef50_UPI001912123E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001912123E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912123E" ], "product": "ABC transporter permease" } }, "id": "CNOCLNGIAL_731", "locus": "GCA000143945_00705" }, { "type": "cds", "sequence": "contig_1", "start": 395422, "stop": 396678, "strand": "+", "frame": 1, "gene": "nosY", "product": "putative protein MPN_335", "db_xrefs": [ "COG:COG1277", "COG:O", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75443", "UniRef:UniRef90_P75443" ], "nt": "ATGGTCGACTTTGTTAGCTTTCGCGATCAAAGACACCTTTACTTTTTAAGTTATTCCTTTTTTATCAATACCCATAACCTGCCCACACAACCGTTACCCCGGATTCTTACTTATGACAATAACGGTGCCTTTTTAGAGAAAATAGTCCCTTCCTTTGATGACATAAAACTAGATCCACCCCGGGTCAAAAAGGTTTACAAATTCTTTAGTGACACCATTATCCAGTCCTTCCGTGATTATGAAGCGGAGGAAAAAGAAAGGCAGGAAAAGGAAGAGAAAGAAAAGGCTGAAAAGGACAATGGCAACGGCCAGGATTCCAATAAAGTTAACTCTGTCAGCACGGAGCCTAACAATAAGAATTCATCAGATGCGGATTCTAAGGATAACAATGATTCATCCGATTCCCAAGGCAAAGACTCAAGTAAGAGTAAGCCTAAGTTTAGACCTAGGCTGCCACAATTCTTTGATCGTGTTTCCATTAACTACAGTAAGTTTTCCAGTAGCTTTAACACACAACTAAAACACATTCATCAAGGACTTTCTACCCGTGATGAGGCCATGGAAATCTTTAAAGATAAGGTAGCGTTATTTTATGCCCTATCAATGGCTTATGACAACTTTACTTTCCAAAAGGACAGTGGCATTATTGATAACACTAGCTTAAACAAATTTAAAACAGAGTTTAACAAGAAGCAAAAAGAGCTAACTGAAAAAAACAAGCAAAAACAGGACGGTAAAGACCAAAAGAGTCAAAGAATGACACAAGGTGCTGATAAAGCAGTCACTGTTGAGCCAAAAGCTATGCAGTCTGAAATTGGAATGACTGATCAAACTAATGACCAAAGCAGTTCGGAAACAAAGGACAGCATGGATAGTTCAGACAGTAGTGATACTGTTGATAACACTGATGAATCAGAAGATAAACAAAGTGAAGAGGAGGAAAAGTTCGACGAGGAAATTGAGAATGCCAAAAAAATGCCTAAGGCTGAAGACGCCTTTTTTAATACTGCCTCTATCTGGTTGAGCTCACCATTTCTCTTTTTTGAAAATGGTGCAAAACGCGAACAACGATACGAAATCCATTTGCTCAATTCAAACACACAGGTTAATAACGAAATAATTAAAACTAACAGCTTTTATTACGTTAGTGGTGAACCGATTGTAGGCCAAGAAGTCATTATTGCAATGGTGCTGGTGGTAACACTAGGTCTGTTAGTTGGTAGTTTCTTCGCTTACCAAAAACGCGATATTAAGTAA", "aa": "MVDFVSFRDQRHLYFLSYSFFINTHNLPTQPLPRILTYDNNGAFLEKIVPSFDDIKLDPPRVKKVYKFFSDTIIQSFRDYEAEEKERQEKEEKEKAEKDNGNGQDSNKVNSVSTEPNNKNSSDADSKDNNDSSDSQGKDSSKSKPKFRPRLPQFFDRVSINYSKFSSSFNTQLKHIHQGLSTRDEAMEIFKDKVALFYALSMAYDNFTFQKDSGIIDNTSLNKFKTEFNKKQKELTEKNKQKQDGKDQKSQRMTQGADKAVTVEPKAMQSEIGMTDQTNDQSSSETKDSMDSSDSSDTVDNTDESEDKQSEEEEKFDEEIENAKKMPKAEDAFFNTASIWLSSPFLFFENGAKREQRYEIHLLNSNTQVNNEIIKTNSFYYVSGEPIVGQEVIIAMVLVVTLGLLVGSFFAYQKRDIK", "aa_hexdigest": "4804eeb1070233fb5a8adc7548ab2efd", "start_type": "ATG", "rbs_motif": null, "genes": [ "nosY" ], "truncated": "5-prime", "pseudogene": { "start": 395122, "stop": 396678, "observations": { "mutation": [ 395308 ], "stop": [ 395308 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75443", "query_cov": 1.0, "subject_cov": 0.5580774365821095, "identity": 1.0, "score": 592.0, "evalue": 9.55e-205, "gene_start": 1, "gene_end": 418, "reference_start": 332, "reference_end": 749, "reference_sequence": "MLLLSVNKMKLFKNWSDVNNVKQRQLLLAYFRFILKQTVKSWLLWVTAGLVLFLLALVLLIIPAFTKQDPLFLWSHPVVQMSSLIIPFIALFATIITFQVFINGYYNGLEILLITRFFTRGRLFFARLAVLFIWITGTAFLSGLFVSLTATLGATSQTVTDLVLSVFFGVLLLSLLFSCVLIIIAQFLNRTQSMLLLLLGVSLASFTTVILAFSIKPPSENLRDNSYQPLNLSLISKSKSKGVENVFTIIDSSNVSDPRNKKKSIVKTNPQAIWDEYGESSFFQSQYYWNVGYWINSLFRLNSLSDYRNFDVNLYFLNTKLNFDREVDTDKMVDFVSFRDQRHLYFLSYSFFINTHNLPTQPLPRILTYDNNGAFLEKIVPSFDDIKLDPPRVKKVYKFFSDTIIQSFRDYEAEEKERQEKEEKEKAEKDNGNGQDSNKVNSVSTEPNNKNSSDADSKDNNDSSDSQGKDSSKSKPKFRPRLPQFFDRVSINYSKFSSSFNTQLKHIHQGLSTRDEAMEIFKDKVALFYALSMAYDNFTFQKDSGIIDNTSLNKFKTEFNKKQKELTEKNKQKQDGKDQKSQRMTQGADKAVTVEPKAMQSEIGMTDQTNDQSSSETKDSMDSSDSSDTVDNTDESEDKQSEEEEKFDEEIENAKKMPKAEDAFFNTASIWLSSPFLFFENGAKREQRYEIHLLNSNTQVNNEIIKTNSFYYVSGEPIVGQEVIIAMVLVVTLGLLVGSFFAYQKRDIK" }, "paralog": false, "identity": 0.9980694980694981, "score": 795.0, "evalue": 3.42e-287, "description": "Internal stop codon at 395308. Nonsense mutation around 395308.", "psc": { "uniref90_id": "UniRef90_P75443", "gene": "NosY", "product": "Uncharacterized protein MPN_335", "uniref50_id": "UniRef50_P75443", "cog_id": "COG1277", "cog_category": "O", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75443", "UniRef:UniRef50_P75443", "COG:COG1277", "COG:O", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75443", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75443" ], "product": "Uncharacterized protein MPN_335" } }, "id": "CNOCLNGIAL_732", "locus": "GCA000143945_00706" }, { "type": "cds", "sequence": "contig_1", "start": 396961, "stop": 397308, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTACAACAGTTTAACGTCCGACTGTTGGGTAAGTGTCCTTTAGATTTGGCATCGAGTGAAATGTTTGGTAGTCATAAATTTCGACAAATTCCCGCTAAGGTGTTGCACTATATCCACCAACACAACATTTATCTAAAAACCATTTTGCAAACACTGTTAGATGAACCGCGCATGCAACACTGTTTGCGCGTTGGTCAGTTAGCTAAAACTTTAGCAGTGGCAAATAAACTCGATGGTAAAACGGCTTATACTGCTGGTGCTTACCATGATCTAGCAAAGCAACTACCCCAAGCACAATTAGAGAAATTAGCCAAAGTAGCGGGTGTAAATGATTACCCTAGCTGA", "aa": "MVQQFNVRLLGKCPLDLASSEMFGSHKFRQIPAKVLHYIHQHNIYLKTILQTLLDEPRMQHCLRVGQLAKTLAVANKLDGKTAYTAGAYHDLAKQLPQAQLEKLAKVAGVNDYPS", "aa_hexdigest": "b0ff642501f2cd9c8351a8382548fdd1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12909.93339999998, "isoelectric_point": 9.48163928985596 }, "id": "CNOCLNGIAL_733", "locus": "GCA000143945_00707" }, { "type": "cds", "sequence": "contig_1", "start": 397384, "stop": 397713, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01467.32" ], "nt": "GTGATCTTTGGTGGTGCATTTGATCCCCTCCACCAAGCGCATATCTACATTGCTAAGCGTGCTGTACAAGCCATTAAAGCACAAAAGCTTTACTTTGTACCAACTGCTAAAGCCTTTTTTAAATCACCGATTAAAGCATCTAATCAAGCACGTTTAGCAATGTTAAGGGTTGCGCTAAAAGCATTGCCTCAGATGGCAGTATCTAACTTTGACATTAAAGCGCAAAACGGTTTTTCGTTCAATACAGTACAACACTTTAAGCAGCGCTTTCCCAATGCTGAGTTGTACTTTTTAATTGGCAGTGATAAATTAAGCGAGTTGGCCAAATGA", "aa": "MIFGGAFDPLHQAHIYIAKRAVQAIKAQKLYFVPTAKAFFKSPIKASNQARLAMLRVALKALPQMAVSNFDIKAQNGFSFNTVQHFKQRFPNAELYFLIGSDKLSELAK", "aa_hexdigest": "63fda029442a289b48af7ffea810f352", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01467.32", "name": "CTP_transf_like", "length": 106, "aa_cov": 0.963302752293578, "hmm_cov": 0.8773584905660378, "evalue": 2.11896592208681e-12, "score": 42.86591720581055, "start": 2, "stop": 106 } ], "seq_stats": { "molecular_weight": 12231.238799999988, "isoelectric_point": 10.246040916442869 }, "id": "CNOCLNGIAL_734", "locus": "GCA000143945_00708" }, { "type": "cds", "sequence": "contig_1", "start": 397798, "stop": 398259, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAAAAAGGAACTAGAAATTTACAACCTCTATACTTTTCAAATAGACCTCGATAAAAAGCTTTTATTTGAAAAAGCAGATAATCAACAGAATTACTCTAAGATTAGAGCTCGTTATTTTAAAAATTCTGCACGTAATCAACAAGCAGTTTTTCTCAATAAAAACCTGATTAAAAACACTTTTAATAAGGCACTGTTAAACTTCTCGGATTTTTTGAGTGGTAGTGGTGTAGAGAGCATTTTTAAGCAGGTCATTGATGATCAACCTGAAGTTTTAAACTACCTCAAACAGGTCAAAAAGGAAGATAGTTGCGATGGTCACAGCGAAGCGTCACAGTTAGTATTTAATGTAGTCATTAACCCTAAAAATACGCTCGCTAACTTCTTTGAGGAATTAACCATTTACCTCCACTTTAATGAAGAGAACAGCACAGTTGTCGGAAGCTTTTCGTTAAAGTGA", "aa": "MVKKELEIYNLYTFQIDLDKKLLFEKADNQQNYSKIRARYFKNSARNQQAVFLNKNLIKNTFNKALLNFSDFLSGSGVESIFKQVIDDQPEVLNYLKQVKKEDSCDGHSEASQLVFNVVINPKNTLANFFEELTIYLHFNEENSTVVGSFSLK", "aa_hexdigest": "74d4e5d709dccd41c377983b0a39602a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17761.914500000003, "isoelectric_point": 6.737716865539551 }, "id": "CNOCLNGIAL_735", "locus": "GCA000143945_00709" }, { "type": "cds", "sequence": "contig_1", "start": 398455, "stop": 398607, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGCAAAAGTACCCTTTTTCCACGATTCACAAAAACTTAAAGATTGTCAATTCTGATTTCTTTGATGCTTTTTTTGTGATCTTGTTGTTAATTTGTCACCTCAATAATAACTTGTTGTGGTTGTGTGAAAAAACTGAGCACTTTGAATGA", "aa": "MEQKYPFSTIHKNLKIVNSDFFDAFFVILLLICHLNNNLLWLCEKTEHFE", "aa_hexdigest": "6526f5c90980bb80098122c6ab14354d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6058.011799999996, "isoelectric_point": 5.81379909515381 }, "id": "CNOCLNGIAL_736", "locus": "GCA000143945_00710" }, { "type": "cds", "sequence": "contig_1", "start": 398668, "stop": 399153, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTGTTTTTAAAGGACTGGTACTTTGAAAACCAAGCCGTTACTAATGAAGACATTGAAAAGGTTGATGAAGTGGAAGACATTGGTAAGTTAGTGGAAAAATACAGCGCTAATCAACCACAAAAGTTGTCTTCTAATTCAACCGTTTATGTATTTGAACCAGACAAAAAACAGTGCTTTTTAAAGAATGATGACTTTTTCAACACTAACGAAGCTAAGCTGTTGTTTTTAATCACAATGCAACCCAATGTTTTTGGTCTGGATGATACTGCCATTGCCAATGACTTAAACCTAAGGGAAATTGGTGACTTCTTTAAGGAAATTGACTTTACTGACAGCGATGTGCTCAATGACTTTCAACAACAAAAAGAAACTTTGTTAGTACGGCGTACCTTTAACCAACTGTTGTTTATGAACAGTAATACCGATGTTTTGAGTATTGTTAACAATAAGTTCAAATCAGCGATCCATAACATTGTCTGA", "aa": "MLLFLKDWYFENQAVTNEDIEKVDEVEDIGKLVEKYSANQPQKLSSNSTVYVFEPDKKQCFLKNDDFFNTNEAKLLFLITMQPNVFGLDDTAIANDLNLREIGDFFKEIDFTDSDVLNDFQQQKETLLVRRTFNQLLFMNSNTDVLSIVNNKFKSAIHNIV", "aa_hexdigest": "1b14b4b571ea121106574fc9cd30a994", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18764.926, "isoelectric_point": 4.459667015075683 }, "id": "CNOCLNGIAL_737", "locus": "GCA000143945_00711" }, { "type": "cds", "sequence": "contig_1", "start": 399178, "stop": 399495, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAAGGCATTTGATTACTCCAAGATCTTTGAGCAAAACCGCACTAACGATCCTTCTCTCTTGCGTTCTAACCTCAACAGCATTAACCGGCTGCGGTATCTCAGTGAATACTTCCGTACCGCTTCAGTTAAGTATGACCAGCTGTACACCAAGGTAAAGGAGTACATGCAATTAGATCAGTTCTTAGTAGACATGATTAACCAGGTCAACCATGAAGATGAGATCTTTGGTAAATACAAGGAACGGGTTTATTTGAGTTTGGGAATAGTTACTGCTGTTGTCTTTGGTATTATTGAGTTCTTTAACTGTGTTTGA", "aa": "MLKAFDYSKIFEQNRTNDPSLLRSNLNSINRLRYLSEYFRTASVKYDQLYTKVKEYMQLDQFLVDMINQVNHEDEIFGKYKERVYLSLGIVTAVVFGIIEFFNCV", "aa_hexdigest": "c5cde1f6fda7d44f9e08dadb56cfb401", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12541.246299999995, "isoelectric_point": 6.566063117980956 }, "id": "CNOCLNGIAL_738", "locus": "GCA000143945_00712" }, { "type": "cds", "sequence": "contig_1", "start": 399666, "stop": 400646, "strand": "+", "frame": 3, "gene": null, "product": "MPN338 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47484", "UniRef:UniRef90_P75440" ], "nt": "ATGGAATTCAATAAGTTACAACTCAGTCACTGTATTTCCTTTTACATTTCAGACGTTTCGGAAGTGTTCTTTGAAAGTATTAACCAACACCCTAGTCGTGATTTTGTCAACAACATCCTGCAAAAGATTAAGAGCACTCTCAGTGAAGAGGAATTGGAAAAGCTCAACACCATTGAGGAAGTTACTAAGGATGAGAAAATTTTGATCATGCTCAACCATGTGTTGAAGAAGATTGTTTCTAAAACGGGTAGTTCTAGCTGTGACTTATTTCAAATAGTGAAACACGACCGCTTTAACGAGCCGGTTTACATCCAAAGTGTCAATGCCTTTGAAAACAATTTGATTAACAATGAATTTGCGGAAAGGCGGTATGACTATTTAATTGAAATTAATAAACACTCTTATTTAAGGAAGTATGTCAATGCCATTCGCATCTCCTTCTTTTTAGATTTAAGGGCGCAAATCTTGTCCGGTTCCTTTACTTTGGATGTGATTAACAAACAGTTGGAACACCAAAATAAGGAAGAGTTGTTCCAAGCGATTTACTTACGCTCCTTAATTAAGCATTTTATCTCGAACCAACCTTACCCGATTTCCTTAAATTCGTTTATTTTTGGTGATAAAAACCGTGAAAACAAGACGGTTGAAAACGACAAGCTTAGTGTGCTTAAAAACAATTGGAATCAAATCTTCTTTTCTAAGTACTTTGATTTTGTTGCCAAAAACAAAGAAGAGCGTGTTGTCGATAACAACTGCGATGAGCTGTTTTACGCCACCATGAACACGCTCTTGATTATGTTGATCATTATTGAAGAGCTCCGGGTATACTTCAACAGTAAAGAGCCAGCCTTAATCTTAAAGCTACTAGATAACAAGGTTTCACTGCGCGAAGACCCCGACCAAAATCCAGAAACTGATTTGCATGAGTTAATTAAGTTTGCTGAAAAGAATTACCTCGAAAAGGAAAAAACAAGTCGCTGA", "aa": "MEFNKLQLSHCISFYISDVSEVFFESINQHPSRDFVNNILQKIKSTLSEEELEKLNTIEEVTKDEKILIMLNHVLKKIVSKTGSSSCDLFQIVKHDRFNEPVYIQSVNAFENNLINNEFAERRYDYLIEINKHSYLRKYVNAIRISFFLDLRAQILSGSFTLDVINKQLEHQNKEELFQAIYLRSLIKHFISNQPYPISLNSFIFGDKNRENKTVENDKLSVLKNNWNQIFFSKYFDFVAKNKEERVVDNNCDELFYATMNTLLIMLIIIEELRVYFNSKEPALILKLLDNKVSLREDPDQNPETDLHELIKFAEKNYLEKEKTSR", "aa_hexdigest": "40dcca5b103ddb45eb80196061ac079e", "start_type": "ATG", "rbs_motif": "AACAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 399666, "stop": 400949, "observations": { "mutation": [ 400644 ], "stop": [ 400644 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75440", "query_cov": 1.0, "subject_cov": 0.5158227848101266, "identity": 0.997, "score": 628.0, "evalue": 2.84e-222, "gene_start": 1, "gene_end": 326, "reference_start": 1, "reference_end": 326, "reference_sequence": "MEFNKLQLSHCISFYISDVSEVFFESINQHPSRDFVNNILQKIKSTLSEEELEKLNTIEEVTKDEKILIMLNHVLKKIVSKTGSSSCDLFQIVKHDRFNEPVYIQSVNAFENNLINNEFAERRYDYLIEINKHSYLRKYVNAIRISFFLDLRAQILSGSFTLDVINKQLEHQNKEELFQAIYLRSLIKHFISNQLYPISLNSFIFGDKNRENKTVENDKLSVLKNNWNQIFFSKYFDFVAKNKEERVVDNNCDELFYATMNTLLIMLIIIEELRVYFNSKEPALILKLLDNKVSLREDPDQNPETDLHELIKFAEKNYLEKEKTSRWHKKRVKSLEELLEEIKQINLETKNESLAYPDEIVELELDNVHNFVSTKQVFRHQLDLQTLHGIVINPERYGIGMWSNHFVDWEEFKDLIEQITDAENGSDLYGFEKDLDESICQVNKKYLTFISSDSSSFLIIKNDQTKVISNYVWAQLYFETRRWIINDIEYDLYEKGFDKSHFASNIALLESLNFNWLDPFYGLTSIKEIMQKIDSKSNLKTSIAEMVAKFKHEQRISKKDNERVLMIFAYVAAAVVGFINFFSMVFTILTVSDLNAGLTPANIVVIAIASLLALFLIVIAVLFRFRWKYIKH" }, "paralog": false, "identity": 0.9953161592505855, "score": 842.0, "evalue": 6.12e-309, "description": "Internal stop codon at 400644. Nonsense mutation around 400644.", "psc": { "uniref90_id": "UniRef90_P75440", "product": "MPN338 family protein", "uniref50_id": "UniRef50_P47484", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75440", "UniRef:UniRef50_P47484", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47484" ], "product": "Uncharacterized protein MG242" } }, "id": "CNOCLNGIAL_739", "locus": "GCA000143945_00713" }, { "type": "cds", "sequence": "contig_1", "start": 400746, "stop": 401213, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAATTAGAGTTAGATAACGTTCACAACTTTGTCTCTACTAAACAGGTTTTTCGTCACCAGTTAGACTTACAAACTTTACATGGTATAGTCATCAACCCTGAGCGCTATGGTATTGGCATGTGGAGTAACCACTTTGTCGATTGGGAGGAGTTTAAAGATTTAATTGAACAGATTACTGATGCTGAAAATGGTAGTGATCTCTATGGTTTTGAAAAGGACTTGGATGAGAGCATTTGCCAAGTTAACAAGAAGTACTTAACCTTTATTAGTAGTGACAGTAGCTCTTTCTTAATTATTAAGAATGACCAAACTAAGGTTATCTCTAATTATGTCTGGGCGCAACTGTACTTTGAAACGCGCCGGTGGATTATTAATGATATTGAATACGATCTGTATGAAAAGGGGTTTGACAAGAGCCACTTTGCTAGTAACATTGCCCTTTTAGAGAGCTTGAATTTTAACTGA", "aa": "MELELDNVHNFVSTKQVFRHQLDLQTLHGIVINPERYGIGMWSNHFVDWEEFKDLIEQITDAENGSDLYGFEKDLDESICQVNKKYLTFISSDSSSFLIIKNDQTKVISNYVWAQLYFETRRWIINDIEYDLYEKGFDKSHFASNIALLESLNFN", "aa_hexdigest": "6f14ab6eb370bebc7a0ac39820e35106", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18331.2391, "isoelectric_point": 4.609608268737793 }, "id": "CNOCLNGIAL_740", "locus": "GCA000143945_00714" }, { "type": "cds", "sequence": "contig_1", "start": 401253, "stop": 401546, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAGATTGACAGTAAGAGTAATTTAAAAACATCGATTGCCGAAATGGTCGCTAAGTTTAAACACGAACAGCGCATCTCCAAAAAGGACAATGAGCGGGTGTTGATGATCTTTGCTTATGTAGCCGCTGCGGTAGTTGGTTTTATTAACTTTTTTTCGATGGTGTTTACCATTCTTACAGTCAGTGATCTCAATGCTGGTCTCACCCCCGCTAACATTGTTGTTATTGCAATTGCGAGCTTATTGGCCTTATTTTTAATTGTTATTGCTGTTTTATTTCGGTTTAGGTGA", "aa": "MQKIDSKSNLKTSIAEMVAKFKHEQRISKKDNERVLMIFAYVAAAVVGFINFFSMVFTILTVSDLNAGLTPANIVVIAIASLLALFLIVIAVLFRFR", "aa_hexdigest": "939c945166e706743f54903491bbe67b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10793.860399999985, "isoelectric_point": 9.991777610778808 }, "id": "CNOCLNGIAL_741", "locus": "GCA000143945_00715" }, { "type": "cds", "sequence": "contig_1", "start": 401564, "stop": 401866, "strand": "+", "frame": 2, "gene": null, "product": "putative MG243-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75439", "UniRef:UniRef90_P75439" ], "nt": "ATGCAGGTTAAAGTAATTGATGAAAACAGCAATACAACCGCTGTCCTTAACTGCGCTAAAGCGAAAACGCGAGCGCTAGCTTGGTTGTGTGATACAGTACTGTTAGCGATCTTACTCGCAATTATTTACGGCATTAGTAGCATTTTCATCAAAGAACAGTCCAGTGTGTTTTTAATTATGACGGTAAGTCAAGCAGTCTTGTGGTTGACTTACTTTGTCATTTTACCGGGTTTATGGAAGGGCAAAACGCTCTTTCGTGCTTTACTGGGTTTAAGTTTACTCATCTTTAAAAAACGCTTTTGA", "aa": "MQVKVIDENSNTTAVLNCAKAKTRALAWLCDTVLLAILLAIIYGISSIFIKEQSSVFLIMTVSQAVLWLTYFVILPGLWKGKTLFRALLGLSLLIFKKRF", "aa_hexdigest": "133ea20dfc5d0d5ac66f93d9121aa6fb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 401564, "stop": 402169, "observations": { "mutation": [ 401864, 401894 ], "stop": [ 401864, 401894 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75439", "query_cov": 1.0, "subject_cov": 0.44642857142857145, "identity": 1.0, "score": 186.0, "evalue": 6.22e-58, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MQVKVIDENSNTTAVLNCAKAKTRALAWLCDTVLLAILLAIIYGISSIFIKEQSSVFLIMTVSQAVLWLTYFVILPGLWKGKTLFRALLGLSLLIFKKRFWNLLVHELFLWVWYSVIFLALAIYFFVNRDDPKILQAFFDNQNSNLSWIFVKILLSVISVLQLVFVVYFCFSSQKQALQDLLSKSFMVQKAIKVKDCKSELKSTNTIKTHSDLPGDIDLEQLGD" }, "paralog": false, "identity": 0.9850746268656716, "score": 382.0, "evalue": 2.24e-137, "description": "Internal stop codon at 401864, 401894. Nonsense mutation around 401864, 401894.", "psc": { "uniref90_id": "UniRef90_P75439", "product": "Uncharacterized protein MG243 homolog", "uniref50_id": "UniRef50_P75439", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75439", "UniRef:UniRef50_P75439", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75439", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75439" ], "product": "Uncharacterized protein MG243 homolog" } }, "id": "CNOCLNGIAL_742", "locus": "GCA000143945_00716" }, { "type": "cds", "sequence": "contig_1", "start": 402023, "stop": 402238, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAGTGTTATTAGTGTCTTACAACTAGTATTTGTGGTGTATTTCTGTTTCAGCTCCCAAAAGCAAGCGTTGCAAGATTTGTTATCTAAGAGTTTTATGGTGCAAAAAACAATTAAAGTAAAGGATTGTAAAAGTGAATTGAAGTCCACTAACACTATAAAAACGCACTCTGATTTACCCGGCGACATTGATTTAGAACAATTAGGTGATTAA", "aa": "MLSVISVLQLVFVVYFCFSSQKQALQDLLSKSFMVQKTIKVKDCKSELKSTNTIKTHSDLPGDIDLEQLGD", "aa_hexdigest": "2defa3eb3389342dfea20488ba5def0e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7981.239799999999, "isoelectric_point": 6.53377857208252 }, "id": "CNOCLNGIAL_743", "locus": "GCA000143945_00717" }, { "type": "cds", "sequence": "contig_1", "start": 402242, "stop": 403759, "strand": "+", "frame": 2, "gene": "uvrD", "product": "putative DNA helicase MPN_340", "db_xrefs": [ "COG:COG0210", "COG:L", "EC:5.6.2.4", "GO:0000725", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0033202", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "SO:0001217", "UniRef:UniRef50_P75438", "UniRef:UniRef90_P75438" ], "nt": "ATGGAACATTTAAATCAGGAACAGAAAGCTGCTGTTACTTGTGATAACGGTGTTAACGTGGTGTATTCCGGTGCTGGCACGGGTAAAACAACCGTTATTGCTGAACGCTTTGCTTACTTAGTCAATGAAAAAGGCGTTAATCCTCAAAGTATTTTGGCCTTTACCTTCACTGATAAGGCAGCGAGTGAAATGCGCCAACGCATTATTAAGTTAATTCCACAAAAAAGCTTGCAGGATCTGCACATCTATACCTTTCACAGTTTTGCCAACAGGTTTTTGCAAAAACATGGCAAAAGTGACTTTGCCATCTTGAGAGATTCTAACCGCTTCTTTAGTGATTATGAAATGGGTGATCAACTACAAACAGTGGTGGAAATTTATAAAAACAAAGTAGTTGATTTAGAACTTGACAACCTAGAGTATAACTCCGCTTTTAGAGATGCTTGTACAGATACTTTTAACGAGGATTTTAGTACCATCTCCAATGGTCAGTTTCGTAAACGGGCTGCGACTGCTTTACGGGCTTACCAGAACTATTTAATTACCAATAACCTATTTGATTTTAGCGATTTAATTATTGAAACTTGCCACCTTTTAAAAGGTAATAGTGAATTGTTGCAAGCATTTACCGAAAGTGTGCATTATATCTTAGTAGATGAATTTCAAGACACTAACCTAGCCCAGTACGAATTGGTAAAGTTACTAGCTACAACTCATCCTAATCTCTTTTTGGTGGGTGATAGCAACCAGATGATTTACGGTTGGCGGGGTGCTGTTGTAGAGATCTTTGAACTTTTAAAAAATGATTTTCAAACAGTCAAAGAGTTTTATACCACCCAAAACTACCGCAGCAGCCAAGCGGTGTTAGCAGTAGCGAATGATGTACTAACCGCAATTGCTAGAAAAGAGAGGAAAGCGCTCGTGTTGTTGCACTCTAGCATTGATTCTAAAGCTGTACCGGTACACTATAAGGCCAATTCTTTGAAAAACCAAGACCAGTGGATTATTTACCAAATGAAACAGTTGCATTTAAACAATGGTGTACCTTATGATCAGATGGCGGTGTTATTTCGTAAGAATAAACACCTTGACGCTTTTAGTCAAACAGTGCTGGAAGATGGTGATTTACCTTTAGCTAAATTAAACCTTTTAACAATTCACGCTGCCAAGGGTTTGGAATTTGAGGCAGTATTTGTTTATGGCTTGGTTGAACGCGCTTTTCCTAGTTTGCACTGGGATGGAAGTGATAAGCACAAGTTACTAGAAGAAATGAAACTGTTTTACGTAGCGATTACTCGTGCTAAACAGTTTTTATTTTTAGTTTCTGTTAGTGTGGAAAGTTTCAATGCGTATTATGAGCCGTCACGCTTTTTAAAACTAATTGAAAAGGAACATTTGCAAACACAAAAGGCAGCGTACTTTAAAGAACAATTAAAGACACAGCCAAAACCAGTTAATTTGTATACAGAGACAGAAAACGCGGAAAACTTACAAAAAGCCACTGACTCTAAAAAGTGA", "aa": "MEHLNQEQKAAVTCDNGVNVVYSGAGTGKTTVIAERFAYLVNEKGVNPQSILAFTFTDKAASEMRQRIIKLIPQKSLQDLHIYTFHSFANRFLQKHGKSDFAILRDSNRFFSDYEMGDQLQTVVEIYKNKVVDLELDNLEYNSAFRDACTDTFNEDFSTISNGQFRKRAATALRAYQNYLITNNLFDFSDLIIETCHLLKGNSELLQAFTESVHYILVDEFQDTNLAQYELVKLLATTHPNLFLVGDSNQMIYGWRGAVVEIFELLKNDFQTVKEFYTTQNYRSSQAVLAVANDVLTAIARKERKALVLLHSSIDSKAVPVHYKANSLKNQDQWIIYQMKQLHLNNGVPYDQMAVLFRKNKHLDAFSQTVLEDGDLPLAKLNLLTIHAAKGLEFEAVFVYGLVERAFPSLHWDGSDKHKLLEEMKLFYVAITRAKQFLFLVSVSVESFNAYYEPSRFLKLIEKEHLQTQKAAYFKEQLKTQPKPVNLYTETENAENLQKATDSKK", "aa_hexdigest": "6a2e59c2226c4d9a1e6b9ea80f47a0f5", "start_type": "ATG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_P75438", "query_cov": 1.0, "subject_cov": 0.9546313799621928, "identity": 0.9940000000000001, "score": 981.0, "evalue": 0.0, "valid": true, "gene": "UvrD", "product": "Probable DNA helicase MPN_340", "ec_ids": [ "5.6.2.4" ], "uniref50_id": "UniRef50_P75438", "cog_id": "COG0210", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0033202", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0000725" ] }, "pscc": { "uniref50_id": "UniRef50_P75438", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75438" ], "product": "Probable DNA helicase MPN_340" }, "genes": [ "uvrD" ], "id": "CNOCLNGIAL_744", "locus": "GCA000143945_00718" }, { "type": "cds", "sequence": "contig_1", "start": 403893, "stop": 404543, "strand": "+", "frame": 3, "gene": null, "product": "ATP-dependent helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013300ABE", "UniRef:UniRef90_UPI0013300ABE" ], "nt": "ATGGCATTTAACATTAGCACTCAACTCAATAAGGAACAACGAGCCGCTGTTACCTGTGGTAAGGGTGTCAACATTGTCTATTCTGGTGCTGGTACTGGTAAGACCACCATTATTTCCCAACGCTTTGCTTATTTATTTAACCAAAAGCGAATTAATCCTAGCAACATTTTGGCGTTAACCTATACAAGGAAAGCGGCGAGTGAGATGAAGCAGCGGATTTTGGAGCTGTTACCGGAAAAGTATCATAAGGATGTCAACATTTACACCTTCCACAGCTTTTGTAGCCGTTTTTTACGTGAAGAAGGGCAAAAAAACTTTGTGATTGATGATGACTTAAGTAACTTTCTAAAAGACTTTCTCAAAGAGAGTGAACTAAAAAGTCAAAAGGTATTACAAATTATTGACGGTTTTAAAAACGCTTACTTTGATTTTGATACCAACAGCTTAAAGGATGATGAAAGATTGGTCGAGTTGTGTGAGTTTCATTTAGACCCCCAAGAGCGTAACTTTCAACTTTTCAAAGAAACCGCTATTGCTGCTTTTGTTGAATATGAAAAAGGTAAACAACAGAACAATAAAATTGACTTTGCTGATTTATTAATTAAAACTTGCACTTTACTGAGCAAAGATCACAAATTATTGCGCAAGTGA", "aa": "MAFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALTYTRKAASEMKQRILELLPEKYHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFLKESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCEFHLDPQERNFQLFKETAIAAFVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRK", "aa_hexdigest": "d196bf69d5447ba638200a87db8c29ec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 403893, "stop": 404846, "observations": { "mutation": [ 404541, 404679 ], "stop": [ 404541, 404679 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013300ABE", "query_cov": 1.0, "subject_cov": 0.494279176201373, "identity": 1.0, "score": 429.0, "evalue": 7.38e-149, "gene_start": 1, "gene_end": 216, "reference_start": 1, "reference_end": 216, "reference_sequence": "MAFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALTYTRKAASEMKQRILELLPEKYHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFLKESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCEFHLDPQERNFQLFKETAIAAFVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRKWSKKFQYILGDEFQDTNQIQYELIKMLASHHQNLFLVGDNNQMIYRWRGAVSDIIDSLKSDFKVRPENEFYITQNYRCDQNILTVANSILGTIYAKENQPDSTKGFLFSAIKSNRLPVYFQASSVEGQHSWIINKIKNLHKNHGIQYKDMAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLALKSLRVWTNVLNKSH" }, "paralog": false, "identity": 0.9936908517350158, "score": 627.0, "evalue": 5.14e-229, "description": "Internal stop codon at 404541, 404679. Nonsense mutation around 404541, 404679.", "psc": { "uniref90_id": "UniRef90_UPI0013300ABE", "product": "ATP-dependent helicase", "uniref50_id": "UniRef50_UPI0013300ABE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013300ABE", "UniRef:UniRef50_UPI0013300ABE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013300ABE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013300ABE" ], "product": "ATP-dependent helicase" } }, "id": "CNOCLNGIAL_745", "locus": "GCA000143945_00719" }, { "type": "cds", "sequence": "contig_1", "start": 404988, "stop": 405179, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTTGTTTCGCACCAACAGAAACATGGATTCGATGACTGAAGCGCTTGAAGCTGATGGCAGTATTCCCCTTAAGCAAAATAAGGGTTTTTTTAAACAGTTAGAGACTTTTAAAAAGGTATTAGTGGCACTCATAACTAGGTCAAACTATGACATTAAACTAGCTTTAAAAAGTTTGCGGGTTTGA", "aa": "MAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLALKSLRV", "aa_hexdigest": "eca3d2b96738a907cdf33187523e4ba2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7218.467499999999, "isoelectric_point": 9.991777610778808 }, "id": "CNOCLNGIAL_746", "locus": "GCA000143945_00720" }, { "type": "cds", "sequence": "contig_1", "start": 405219, "stop": 405998, "strand": "+", "frame": 3, "gene": null, "product": "ATP-dependent helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310682", "UniRef:UniRef90_UPI0013310682" ], "nt": "GTGAATTTAGATAAAATCTTGCAAAACTTAGAGCAAGCCATTTTTTTAGATGAGCATACTCGGGGTGAGTTGACGGAAGCTGCTAAAGTTTTTACGCGTTTAATTAAGTTTGTTGAAGAACAACAGTTTGAAGCTTTATTAGCCTTTACTTTTGAGGCATTAAATACTGATCAGTTTGTCTGCAACTTTATTTTTAGAACATTGCAAAAACTGCAAACTGAAAATAAAAACTTCACCATTACCGATTTTATTAACGAGTTAAGGTTCCAACAAGATGAGTTAAAGCAGTCCAAAGATAATGTGATTAATTTAATTACAGTGCACTCTGCTAAGGGCTTGGAATTTGAAGCTGTCTTTATTTATGGGATCAATCAAGACAACTTTCCGCTCAAATCGAAGAATCAAATAATAGATCTACAAAGAGAATTGGATGAACTACGCCTTTTTTATGTGGCCTTGACAAGGGCAAAGAAATACTTGTTTTTACTTTCAGTTTATCAAATGAGTGGTGAAATTATTTACCCCTCTAAATTCATTCGTTTTATCAATAAAGAGGATAGACTAGAAATTGCCACCATTAACCATAAAGTTCACCAGGATGATGAATTCTTCGATTCTTCTAAAACAGAAGACTATCAAAAAAAGTATTTAACAGAAAATACGGATTTTGTTAATGGTGACTCTGTTAGCCACCGTACTTATGGTAAGGGCGTTGTTGTTGAAGTTAGAGAAGATGCAGTCTGTGTCGCTTTTAAAAACAGTAAGTACGGACAGAGGTGA", "aa": "MNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFVEEQQFEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVINLITVHSAKGLEFEAVFIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFLLSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVHQDDEFFDSSKTEDYQKKYLTENTDFVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQR", "aa_hexdigest": "243e4dd0ac70085592bd8a9bfcd458a3", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 405039, "stop": 406040, "observations": { "mutation": [ 405177, 405996 ], "stop": [ 405177, 405996 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013310682", "query_cov": 1.0, "subject_cov": 0.7777777777777778, "identity": 0.996, "score": 501.0, "evalue": 5.51e-178, "gene_start": 1, "gene_end": 259, "reference_start": 61, "reference_end": 319, "reference_sequence": "LEADCSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLALKSLRVWPNVLNKSLTVNEQVNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFVEEQQFEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVINLITVHSAKGLEFEAVFIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFLLSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVHQDDEFFDSSKTEDYQKKYLTENTDFVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQRWIVKNHRDLVKAVY" }, "paralog": false, "identity": 0.990990990990991, "score": 642.0, "evalue": 6.77e-236, "description": "Internal stop codon at 405177, 405996. Nonsense mutation around 405177, 405996.", "psc": { "uniref90_id": "UniRef90_UPI0013310682", "product": "ATP-dependent helicase", "uniref50_id": "UniRef50_UPI0013310682", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013310682", "UniRef:UniRef50_UPI0013310682" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013310682", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310682" ], "product": "ATP-dependent helicase" } }, "id": "CNOCLNGIAL_747", "locus": "GCA000143945_00721" }, { "type": "cds", "sequence": "contig_1", "start": 406529, "stop": 407161, "strand": "+", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N2BQM8" ], "nt": "TTGCGCGATATCTTCGATGATATTGAAAAGTCTACTGAACTAGGTGATGGAAGAAGTAAAGAATCCTTTAAAGGTCTTTTCAAAGACTTTAATGTTTCTGAGGTTAAGTTAGGTTCTACTTTAACGATCCGAACAGAGAAATTAAAAGAACTTTTAACAAGCATTGACACAATGGAGCTCGATGAATTTGAGAAAAATTCTATCGATGCATTTGGTGATGCATATGAGTTTTTAATTTCCATGTATGCACAAAATGCTGGAAAAAGTGGGGGTGAGTTTTTTACACCCCAAGATGTATCTGAACTTCTCGCTAGAATTGCGATTGGCAAAAAGGACACCGTTGATGATGTTTATGACATGGCTTGTGGCAGTGGTTCTTTACTTTTACAAGTAATTAAAGTTTTAGGTAAAGAAAAAACTTCACTGGTTAGCTATTACGGTCAGGAAATAAACCACACAACTTACAACCTTTGTCGGATGAATATGATTCTCCACAATATTGATTACGCTAATTTCAATATTATTAACGCTGATACTTTAACAACGAAGGAGTGGGAGAAGCATTACGTAAATTGCTCAAATGAAAATGGGTTTGAAGTTGTGGTTTCCAATCCACCATATTCCATTAGTTGA", "aa": "MRDIFDDIEKSTELGDGRSKESFKGLFKDFNVSEVKLGSTLTIRTEKLKELLTSIDTMELDEFEKNSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDVSELLARIAIGKKDTVDDVYDMACGSGSLLLQVIKVLGKEKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNIINADTLTTKEWEKHYVNCSNENGFEVVVSNPPYSIS", "aa_hexdigest": "7ffbfab5b7e041f92c69c9f8afe28638", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_N2BQM8", "query_cov": 0.8857142857142857, "subject_cov": 0.9946524064171123, "identity": 0.5429999999999999, "score": 176.0, "evalue": 8.36e-53, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_N2BQM8", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_N2BQM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N2BQM8" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "CNOCLNGIAL_748", "locus": "GCA000143945_00722" }, { "type": "cds", "sequence": "contig_1", "start": 407219, "stop": 407812, "strand": "+", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132HPU2" ], "nt": "TTGGCACCTAATTCAAAAGCTGATTTAGCTTTTGTTTTACATGCTCTATATGTATTAGGTCAAGAAGGTACAGCAGCAATTGTTTGTTTCCCTGGAATCTTGTACCGTGAAGGTAAAGAGCAAACCATTAGAAAATACCTAGTTGATCAAAACTTTGTTGATGCAGTGATCCAATTACCAAGTAATCTTTTTAGCACAACATCAATCGCTACTTCAATTCTTGTTCTCAAGAAAAATCGTGATAAAAAAGATCCCATTTTCTTCATTGATGGTTCTAACGAATTTGTTAGAGAAAAGAAGAATAACCGCTTAAGTCCAAAAAATATTGAAAAGATTGTTGATTGCTTTAATAGCAAAAAAGAAGAAGCTAATTTTGCAAAGGCGGTTGAAAGGGACAAAATTAGGGAATCTAACTACGACCTTACAGTAGGTAAATACGTAAACTCAGAGGCTGAAAAGGAAGAATTAGATATTAAGGTTTTGAATCACTCTATTGATGAAATAGTAGATAAACAAAAAGATCTAAGAACTAAAATTAAGGATATTATTCAGGATATCAAAGTAGATTTTGACAACATTGATATTAATAACTAG", "aa": "MAPNSKADLAFVLHALYVLGQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVDAVIQLPSNLFSTTSIATSILVLKKNRDKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKEEANFAKAVERDKIRESNYDLTVGKYVNSEAEKEELDIKVLNHSIDEIVDKQKDLRTKIKDIIQDIKVDFDNIDINN", "aa_hexdigest": "b7090f8053296bb5eac7d605b83109b1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028898C0F", "query_cov": 0.9593908629441624, "subject_cov": 0.8873239436619719, "identity": 0.508, "score": 179.0, "evalue": 1.45e-53, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A132HPU2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A132HPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132HPU2" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "CNOCLNGIAL_749", "locus": "GCA000143945_00723" }, { "type": "cds", "sequence": "contig_1", "start": 407882, "stop": 408061, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGCAAATTAAAACTTACAAAATCAAGGATATTTGCGAGATCTCAAGAGGCAAAGCAATTACTAAAAAATACATTAAGGATAATCCTGGTCAATATCCGGTTTATTCATCTACTACTGCTAATAACGGTGAAATTGGCAGAATAAAGGATTACGATCTTGATGGCGAATACGTTACCTGA", "aa": "MQIKTYKIKDICEISRGKAITKKYIKDNPGQYPVYSSTTANNGEIGRIKDYDLDGEYVT", "aa_hexdigest": "ab2463771057210eb59fcb3706209e77", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 60, "aa_cov": 0.9491525423728814, "hmm_cov": 0.9833333333333333, "evalue": 7.349456625333381e-12, "score": 41.26675796508789, "start": 4, "stop": 59 } ], "seq_stats": { "molecular_weight": 6735.562900000004, "isoelectric_point": 8.708276557922364 }, "id": "CNOCLNGIAL_750", "locus": "GCA000143945_00724" }, { "type": "cds", "sequence": "contig_1", "start": 408191, "stop": 408604, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGGAGGCTCCTAAATTTGTTAATAATGCTTGTCCTATTCCTAACCTCAATTTATCAAGAACTGAGGAAATTGAATTAGATTTCCCACCCTTGCAAATTCAGCAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGA", "aa": "MEAPKFVNNACPIPNLNLSRTEEIELDFPPLQIQQKIATILDTFTELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYIT", "aa_hexdigest": "a6da9a14205d811eaf0fab804d797772", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.4233576642335766, "hmm_cov": 0.9838709677419355, "evalue": 1.1163139135629176e-21, "score": 73.21320343017578, "start": 80, "stop": 137 } ], "seq_stats": { "molecular_weight": 15855.740199999982, "isoelectric_point": 5.107972526550292 }, "id": "CNOCLNGIAL_751", "locus": "GCA000143945_00725" }, { "type": "cds", "sequence": "contig_1", "start": 408791, "stop": 408958, "strand": "+", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00190F3D87", "UniRef:UniRef90_UPI00190F3D87" ], "nt": "ATGGCTGAAATTGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAATTTCTTATTTAATTGA", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "bbbc1991845ad67ac2a240d1c026608d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 408644, "stop": 409003, "observations": { "mutation": [ 408956 ], "stop": [ 408956 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00190F3D87", "query_cov": 1.0, "subject_cov": 0.46218487394957986, "identity": 1.0, "score": 114.0, "evalue": 1.06e-31, "gene_start": 1, "gene_end": 55, "reference_start": 50, "reference_end": 104, "reference_sequence": "GKCNASQDCGVLKVKNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.9831932773109243, "score": 231.0, "evalue": 4.67e-80, "description": "Internal stop codon at 408956. Nonsense mutation around 408956.", "psc": { "uniref90_id": "UniRef90_UPI00190F3D87", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00190F3D87", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00190F3D87", "EC:3.1.21.3", "UniRef:UniRef50_UPI00190F3D87", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00190F3D87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00190F3D87" ], "product": "restriction endonuclease subunit S" } }, "id": "CNOCLNGIAL_752", "locus": "GCA000143945_00726" }, { "type": "cds", "sequence": "contig_1", "start": 409226, "stop": 409876, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_344", "db_xrefs": [ "RefSeq:WP_014325475.1", "SO:0001217", "UniParc:UPI0001DD653A", "UniRef:UniRef100_A0AAX0SQN8", "UniRef:UniRef50_P75100", "UniRef:UniRef90_P75434" ], "nt": "ATGCCTAAAATTATTTCTAAACGAGAATTCAACATTCTTAGAAAAACAAAAGAAGAAATCTTTTCTCAAATTGTTTACACTAAGAAGAGAAACGGTCTGCACAAGGCTGCCAAAGAATATTACTTCAAGGACAAAGACTTTACAATAATAGAAGATCAACAGGATCGACCTGATAAACCTGAGGAACTGGACACACCGGATATTCCAAAGCCACCAAAACCTCCCAAGGGTCCTGATCAACCCGAGGAACCAGGCCAACCAGGTGGTCCCGATGATCCTAATTCAGGAAATAAAAAGATGCCAAAGCCAGACGAATTTGTAACCCACAGACAACTTCAAGAATTTAAGAAGGATCTTTTAGTGGAACTTCACGAAATCTTTCCTACAAAACCTGAATTAAAACGGGTTGAAGAAAAAGTTGATGTTCTGTTTGAACTTCAAAAAGCTCAGGGTGAACAAATAAGAATCCAAGGTGAACAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGCGAAACCCTACAGCTAATCTTGCAAACACTACAAAAGATGAACGATCGTCTGGATAAGATCGAAGGTAAAATGGACAAAATGGAAAGTAGAATGGATAAAATGGAAACTCGCTTAGACAAACTAGAGTCCAAATAA", "aa": "MPKIISKREFNILRKTKEEIFSQIVYTKKRNGLHKAAKEYYFKDKDFTIIEDQQDRPDKPEELDTPDIPKPPKPPKGPDQPEEPGQPGGPDDPNSGNKKMPKPDEFVTHRQLQEFKKDLLVELHEIFPTKPELKRVEEKVDVLFELQKAQGEQIRIQGEQIKELKVEQKAQGETLQLILQTLQKMNDRLDKIEGKMDKMESRMDKMETRLDKLESK", "aa_hexdigest": "34ea9fd1a79390b9fa9a44c828a1dc24", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD653A", "ncbi_nrp_id": "WP_014325475.1", "uniref100_id": "UniRef100_A0AAX0SQN8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD653A", "RefSeq:WP_014325475.1", "UniRef:UniRef100_A0AAX0SQN8" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQN8", "uniref90_id": "UniRef90_P75434" }, "psc": { "uniref90_id": "UniRef90_P75434", "product": "UPF0134 protein MPN_344", "uniref50_id": "UniRef50_P75100" }, "pscc": { "uniref50_id": "UniRef50_P75100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75100" ], "product": "UPF0134 protein MPN_013" }, "genes": [], "id": "CNOCLNGIAL_753", "locus": "GCA000143945_00727" }, { "type": "cds", "sequence": "contig_1", "start": 411111, "stop": 411440, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "TTGGCTAGTGAAATAGACTTTGTTGATAAAGTCCTTTTCGTTGTTGACAGGAAAGACTTGGACAACCAAACGATCGAAGAATATGAAAAGTTTCAAGCGGGTTCTGTTAGCGAAACTGAGAACACAAATGATCTTAAAGAAAAGATATTAGACGATAGTACCGCCACACGTGCAATTGTTACTACTATTCATAAACTTAAGAGGTTAATAGATCAGAGAAGTAAGCTTAAAGACGAAGATCTTAAAAAGAAAAACATCGTTCTTATATTCGATGAATGTCATCGATCTCAATTTGGAAAAATGAAACAAGAAATCGATGAGTTTTTTTAA", "aa": "MASEIDFVDKVLFVVDRKDLDNQTIEEYEKFQAGSVSETENTNDLKEKILDDSTATRAIVTTIHKLKRLIDQRSKLKDEDLKKKNIVLIFDECHRSQFGKMKQEIDEFF", "aa_hexdigest": "b623ee7f176d34416f914f4348479b33", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 100, "aa_cov": 0.8899082568807339, "hmm_cov": 0.93, "evalue": 2.2653916037408494e-07, "score": 26.185123443603516, "start": 10, "stop": 106 } ], "seq_stats": { "molecular_weight": 12799.364999999987, "isoelectric_point": 5.0787572860717765 }, "id": "CNOCLNGIAL_754", "locus": "GCA000143945_00728" }, { "type": "cds", "sequence": "contig_1", "start": 411570, "stop": 411917, "strand": "+", "frame": 3, "gene": null, "product": "Putative type I restriction enzyme MpnIIP endonuclease subunit middle part", "db_xrefs": [ "RefSeq:WP_010874702.1", "SO:0001217", "UniParc:UPI000013A844", "UniRef:UniRef100_P75432", "UniRef:UniRef50_P75432", "UniRef:UniRef90_P75432" ], "nt": "ATGTCTGATGCTTTGGATGACGAAAATGTACTTTGTTTTAGGTATAAGAGTTATTACATTAAAGATGATGGAAGCCGAATTGATACATTAAATGAAGAGAGAATTAAGCATATTGTTGATTTCACTTTAGATAAATATCTAGATGCAACACAAAATACTCCGAAATTTGATAATTTTGAAAATCCTGTAAAAGAAGGGTTTAATTCGATTTTTGCCTGTGAGAGTAAAGAAGCAGCAATCGAATACTATAAAGAATTCAAAAAACAAATTGAACAAAAGAAGCTACCAATTAAAATTGCAAGCATTATAGCGCAAACGAAGGTAGTGATGAACAAGAAATTAGTGTAA", "aa": "MSDALDDENVLCFRYKSYYIKDDGSRIDTLNEERIKHIVDFTLDKYLDATQNTPKFDNFENPVKEGFNSIFACESKEAAIEYYKEFKKQIEQKKLPIKIASIIAQTKVVMNKKLV", "aa_hexdigest": "baa01d1bcd6606b7f793ac25aacdf3f2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A844", "ncbi_nrp_id": "WP_010874702.1", "uniref100_id": "UniRef100_P75432", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A844", "RefSeq:WP_010874702.1", "UniRef:UniRef100_P75432" ] }, "ips": { "uniref100_id": "UniRef100_P75432", "uniref90_id": "UniRef90_P75432" }, "psc": { "uniref90_id": "UniRef90_P75432", "product": "Putative type I restriction enzyme MpnIIP endonuclease subunit middle part", "uniref50_id": "UniRef50_P75432" }, "pscc": { "uniref50_id": "UniRef50_P75432", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75432" ], "product": "Putative type I restriction enzyme MpnIIP endonuclease subunit middle part" }, "genes": [], "id": "CNOCLNGIAL_755", "locus": "GCA000143945_00729" }, { "type": "cds", "sequence": "contig_1", "start": 413300, "stop": 413434, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574953.1", "SO:0001217", "UniParc:UPI0001DD653D", "UniRef:UniRef100_A0AB38W8M6", "UniRef:UniRef50_A0AB38W8M6", "UniRef:UniRef90_A0AB38W8M6" ], "nt": "ATGGAAAAGAAAAATAAAAAAAATACTGCAACCGTTCCCACCAAAGAACAATTGGATGGCATGTTTAAATCTCTTGAAAAAGAAGAGAACTATGATGTCTTTGTTCAAGTCATAAAAGCTTTAGGTAAGATGTAG", "aa": "MEKKNKKNTATVPTKEQLDGMFKSLEKEENYDVFVQVIKALGKM", "aa_hexdigest": "9b3c3574738bab55abda7bca2ce6156a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD653D", "ncbi_nrp_id": "WP_014574953.1", "uniref100_id": "UniRef100_A0AB38W8M6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD653D", "RefSeq:WP_014574953.1", "UniRef:UniRef100_A0AB38W8M6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8M6", "uniref90_id": "UniRef90_A0AB38W8M6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8M6", "uniref50_id": "UniRef50_A0AB38W8M6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8M6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5089.902800000002, "isoelectric_point": 9.050087928771973 }, "id": "CNOCLNGIAL_756", "locus": "GCA000143945_00730" }, { "type": "cds", "sequence": "contig_1", "start": 413437, "stop": 413991, "strand": "+", "frame": 1, "gene": null, "product": "Death-on-curing family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S2Z8", "UniRef:UniRef90_A0AAX0S2Z8" ], "nt": "GTGCGTGAAATAAACGTTTATTATTTAAGCAACGACTCAAAAAGTGATAAAGACGATATCGATTATTTAATAGAAGAACTTTTAGTTGAAGGTTCTCGTTTTAAAAATAGTCTTGTTACGTTTTTAGAAAAAGAAAAAGACTGCCAATCTTGCATTTGTTTATTTAAATCTAAGCTTTCTTCTTCATTCGAAATAAGAGTCAAATTTAATGAATTAAATTTAGATGCAAGTGATGAAAAAACTAAGGAATTTTTAAGATACATAGTTAGTAAATCAATAAACAATGCAAAATTCCTATCTTCTGAAGACTTTGATGACAAAAAAGAAGACGAAATTCTAAGCATCATTGAGAATACCTTTAATAGTTATTGCTACAAAAGCGATTTACTAAGTCCAAGTTTGTATTGTAAAGAAGGCCGCTTGAACATAATTGATTTAATTAACGATATTTTTTGCAAGGTTTTAACTTTTCATAAATTGTCGAACGGAAATAAAAGACTAGCTACAGCCTTATTAGTTAATTTTCTTTACACTTTTGGATATTATTTCAAATGA", "aa": "MREINVYYLSNDSKSDKDDIDYLIEELLVEGSRFKNSLVTFLEKEKDCQSCICLFKSKLSSSFEIRVKFNELNLDASDEKTKEFLRYIVSKSINNAKFLSSEDFDDKKEDEILSIIENTFNSYCYKSDLLSPSLYCKEGRLNIIDLINDIFCKVLTFHKLSNGNKRLATALLVNFLYTFGYYFK", "aa_hexdigest": "a7549db1352a692700b89a30949b4d6d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 413437, "stop": 414171, "observations": { "mutation": [ 413989, 414151 ], "stop": [ 413989, 414151 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0S2Z8", "query_cov": 1.0, "subject_cov": 0.7540983606557377, "identity": 1.0, "score": 360.0, "evalue": 6.24e-125, "gene_start": 1, "gene_end": 184, "reference_start": 1, "reference_end": 184, "reference_sequence": "MREINVYYLSNDSKSDKDDIDYLIEELLVEGSRFKNSLVTFLEKEKDCQSCICLFKSKLSSSFEIRVKFNELNLDASDEKTKEFLRYIVSKSINNAKFLSSEDFDDKKEDEILSIIENTFNSYCYKSDLLSPSLYCKEGRLNIIDLINDIFCKVLTFHKLSNGNKRLATALLVNFLYTFGYYFKWTIPLKKEEYFKYAHMIKNFVVSFEEHKRENRDEFLAKQTKKFIMDNILLALNFWETKLN" }, "paralog": false, "identity": 0.9877049180327869, "score": 479.0, "evalue": 3.53e-174, "description": "Internal stop codon at 413989, 414151. Nonsense mutation around 413989, 414151.", "psc": { "uniref90_id": "UniRef90_A0AAX0S2Z8", "product": "Death-on-curing family protein", "uniref50_id": "UniRef50_A0AAX0S2Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0S2Z8", "UniRef:UniRef50_A0AAX0S2Z8" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAX0S2Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S2Z8" ], "product": "Death-on-curing family protein" } }, "id": "CNOCLNGIAL_757", "locus": "GCA000143945_00731" }, { "type": "cds", "sequence": "contig_1", "start": 414034, "stop": 414153, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAAAAACTTTGTAGTTAGTTTTGAAGAACATAAAAGAGAAAACAGGGATGAATTTCTAGCAAAGCAAACAAAGAAATTTATCATGGACAACATCCTTTTAGCTCTCAATTTTTGA", "aa": "MIKNFVVSFEEHKRENRDEFLAKQTKKFIMDNILLALNF", "aa_hexdigest": "2f3b8c462bd921c9950515ac9f9d514c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4758.521099999999, "isoelectric_point": 8.162164878845214 }, "id": "CNOCLNGIAL_758", "locus": "GCA000143945_00732" }, { "type": "cds", "sequence": "contig_1", "start": 414209, "stop": 414703, "strand": "+", "frame": 2, "gene": "fAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "db_xrefs": [ "COG:COG0212", "COG:H", "EC:6.3.3.2", "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872", "RefSeq:WP_010874704.1", "SO:0001217", "UniParc:UPI00001394EC", "UniRef:UniRef100_P75430", "UniRef:UniRef50_P75430", "UniRef:UniRef90_P75430" ], "nt": "ATGGACAAAAATGCCTTAAGAAAACAAATTCTGCAAAAAAGAATGGCATTAAGTACCATTGAAAAAAGTCACTTAGATCAAAAGATTAATCAAAAATTAGTTGCTTTTTTAACTCCTAAGCCATGCATTAAAACAATTGCACTTTATGAACCCATTAAAAATGAGGTTACTTTTGTTGACTTCTTCTTTGAGTTTTTAAAGATTAACCAAATAAGAGCTGTTTACCCCAAGGTAATAAGTGATACCGAAATTATCTTTATTGATCAGGAGACAAATACATTCGAACCTAATCAAATAGACTGCTTCCTAATACCATTGGTAGGCTTTAATAAAGACAATTACCGTCTAGGCTTTGGCAAGGGCTATTATGACCGTTATTTAATGCAATTAACTAGACAACAACCTAAAATAGGAATAGCGTACAGTTTCCAAAAAGGTGATTTTTTAGCCGATCCTTGGGATGTACAACTAGACTTAATTATTAATGATGAATAG", "aa": "MDKNALRKQILQKRMALSTIEKSHLDQKINQKLVAFLTPKPCIKTIALYEPIKNEVTFVDFFFEFLKINQIRAVYPKVISDTEIIFIDQETNTFEPNQIDCFLIPLVGFNKDNYRLGFGKGYYDRYLMQLTRQQPKIGIAYSFQKGDFLADPWDVQLDLIINDE", "aa_hexdigest": "a9956fc88c9aea3f4484912cbd983518", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394EC", "ncbi_nrp_id": "WP_010874704.1", "uniref100_id": "UniRef100_P75430", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394EC", "RefSeq:WP_010874704.1", "UniRef:UniRef100_P75430" ] }, "ips": { "uniref100_id": "UniRef100_P75430", "uniref90_id": "UniRef90_P75430" }, "psc": { "uniref90_id": "UniRef90_P75430", "gene": "FAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "ec_ids": [ "6.3.3.2" ], "uniref50_id": "UniRef50_P75430", "cog_id": "COG0212", "cog_category": "H", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75430", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75430" ], "product": "5-formyltetrahydrofolate cyclo-ligase" }, "genes": [ "fAU1" ], "id": "CNOCLNGIAL_759", "locus": "GCA000143945_00733" }, { "type": "cds", "sequence": "contig_1", "start": 414693, "stop": 415538, "strand": "+", "frame": 3, "gene": "ymdB", "product": "Putative phosphatase/phosphodiesterase MPN_349", "db_xrefs": [ "COG:COG1692", "COG:T", "EC:3.1.-.-", "GO:0004113", "GO:0046872", "KEGG:K09769", "RefSeq:WP_010874705.1", "SO:0001217", "UniParc:UPI00001394F4", "UniRef:UniRef100_P75429", "UniRef:UniRef50_P75429", "UniRef:UniRef90_P75429" ], "nt": "ATGATGAATAGCATTAAATTCATTTTCCTAGGTGACGTCTATGGCAAGGCCGGACGTAATATTATTAAGAATAATCTAGCCCAACTCAAATCAAAGTATCAAGCTGATTTAGTCATTGTTAATGCCGAAAATACGACTCACGGTAAAGGACTTAGTTTAAAGCACTATGAGTTTCTAAAAGAGGCAGGCGTTAATTACATTACTATGGGTAATCATACCTGGTTTCAGAAGCTAGATTTAGCTGTTGTAATTAATAAAAAGGACTTAGTGCGTCCTTTAAACTTAGACACCAGTTTTGCTTTTCATAACTTAGGGCAAGGTAGTCTTGTATTTGAATTTAATAAAGCAAAGATTAGGATAACTAATTTACTGGGTACTAGTGTGCCCTTACCCTTTAAAACAACTAATCCCTTTAAAGTTTTAAAAGAACTAATTTTAAAGCGCGATTGTGATCTTCATATAGTTGATTTTCACGCTGAAACCACCAGTGAAAAAAACGCCTTTTGCATGGCTTTTGATGGTTATGTCACAACCATTTTCGGCACCCACACCCACGTGCCTAGTGCTGATTTAAGAATCACCCCCAAAGGTAGTGCTTACATTACCGATGTCGGGATGTGTGGTCCGGGATTTGGTAGTGTTATTGGTGCTAACCCCGAACAGTCGATCAGACTCTTTTGTGCTGGTAGCAGAGAACACTTTGAGGTATCTAAATGCGGAGCACAACTCAATGGTGTCTTCTTTGAGGTTGATGTAAATACAAAAAAGGTGATTAAAACAGAGGCGATTAGGATTGTGGAGGACGATCCTAGATACTTAAAACAAGACTACTTTAACCTAATTTAA", "aa": "MMNSIKFIFLGDVYGKAGRNIIKNNLAQLKSKYQADLVIVNAENTTHGKGLSLKHYEFLKEAGVNYITMGNHTWFQKLDLAVVINKKDLVRPLNLDTSFAFHNLGQGSLVFEFNKAKIRITNLLGTSVPLPFKTTNPFKVLKELILKRDCDLHIVDFHAETTSEKNAFCMAFDGYVTTIFGTHTHVPSADLRITPKGSAYITDVGMCGPGFGSVIGANPEQSIRLFCAGSREHFEVSKCGAQLNGVFFEVDVNTKKVIKTEAIRIVEDDPRYLKQDYFNLI", "aa_hexdigest": "f7efcd5505e4e1a5cd1366968b50b3de", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394F4", "ncbi_nrp_id": "WP_010874705.1", "uniref100_id": "UniRef100_P75429", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394F4", "RefSeq:WP_010874705.1", "UniRef:UniRef100_P75429" ] }, "ips": { "uniref100_id": "UniRef100_P75429", "uniref90_id": "UniRef90_P75429" }, "psc": { "uniref90_id": "UniRef90_P75429", "gene": "YmdB", "product": "Putative phosphatase/phosphodiesterase MPN_349", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75429", "cog_id": "COG1692", "cog_category": "T", "kegg_orthology_id": "K09769", "go_ids": [ "GO:0004113", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75429", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75429" ], "product": "Putative phosphatase/phosphodiesterase MPN_349" }, "genes": [ "ymdB" ], "id": "CNOCLNGIAL_760", "locus": "GCA000143945_00734" }, { "type": "cds", "sequence": "contig_1", "start": 415732, "stop": 415914, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGCTAAAATAAAGAAGGTAACTAAGCTAGCTAAAGAAACGTACTTGGTTATTAAAGTAACTAGCAATCACAAAACTAAGCAAATAACAAATCACCACAGCGAAATTGCCAGCAAAGAACCGCCTGTAGTAGCGATGGCTTTACCACCTTTAAACTTAAAGTACAGTGGAAAAATATGA", "aa": "MIAKIKKVTKLAKETYLVIKVTSNHKTKQITNHHSEIASKEPPVVAMALPPLNLKYSGKI", "aa_hexdigest": "7e73270642d0ba9ca62ab575e34a0813", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6680.9648, "isoelectric_point": 10.06707668304443 }, "id": "CNOCLNGIAL_761", "locus": "GCA000143945_00735" }, { "type": "cds", "sequence": "contig_1", "start": 415827, "stop": 416249, "strand": "-", "frame": 1, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "db_xrefs": [ "COG:COG0344", "COG:I", "EC:2.3.1.15", "EC:2.3.1.275", "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772", "KEGG:K08591", "SO:0001217", "UniRef:UniRef50_P75428", "UniRef:UniRef90_P75428" ], "nt": "ATGAACGCAGCTAGCGCCATCGCTCTACTTATTGTCTTTAGCCTTGTTATTGGCTATTTAATGGGTTCAGTGATGTTTGCTGATGTTTTTGGCAAGATCTTAAACAAAGATGTCAGGAAATTGGGCTCCAAGAATCCGGGGGCTACTAACTCAATCCGTGTTTTTGGTTTGAAAATAGGCTTCTTAGTTGGTTTATGTGATGCTTTAAAGGGGTTTTTGGCGTTTGTTTTCAGCTTTTTAATCTTTAGCTTTTGGTTACAACAATACCTTAATGTAAACCAGTATCAAAAGGTTTATTACCTCACTTATTTAAGTTGTTTTGCAGCTACCATTGGTCATATTTTTCCACTGTACTTTAAGTTTAAAGGTGGTAAAGCCATCGCTACTACAGGCGGTTCTTTGCTGGCAATTTCGCTGTGGTGA", "aa": "MNAASAIALLIVFSLVIGYLMGSVMFADVFGKILNKDVRKLGSKNPGATNSIRVFGLKIGFLVGLCDALKGFLAFVFSFLIFSFWLQQYLNVNQYQKVYYLTYLSCFAATIGHIFPLYFKFKGGKAIATTGGSLLAISLW", "aa_hexdigest": "0c841b5f00599d8a747333815bfa2b44", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "plsY" ], "truncated": "5-prime", "pseudogene": { "start": 415530, "stop": 416249, "observations": { "mutation": [ 415598, 415622, 415805, 415829 ], "stop": [ 415598, 415622, 415805, 415829 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75428", "query_cov": 1.0, "subject_cov": 0.5857740585774058, "identity": 1.0, "score": 270.0, "evalue": 3.75e-90, "gene_start": 1, "gene_end": 140, "reference_start": 1, "reference_end": 140, "reference_sequence": "MNAASAIALLIVFSLVIGYLMGSVMFADVFGKILNKDVRKLGSKNPGATNSIRVFGLKIGFLVGLCDALKGFLAFVFSFLIFSFWLQQYLNVNQYQKVYYLTYLSCFAATIGHIFPLYFKFKGGKAIATTGGSLLAISLWWFVICLVLWLLVTLITKYVSLASLVTFFILAIIILVPWLDYLYFFKPNPINAISYQNDWYIILFFVLWYWPLTIAVFWLHRKNIHRLLNKTENKVTQLN" }, "paralog": false, "identity": 0.9832635983263598, "score": 464.0, "evalue": 6.72e-169, "description": "Internal stop codon at 415598, 415622, 415805, 415829. Nonsense mutation around 415598, 415622, 415805, 415829.", "psc": { "uniref90_id": "UniRef90_P75428", "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "ec_ids": [ "2.3.1.15", "2.3.1.275" ], "uniref50_id": "UniRef50_P75428", "cog_id": "COG0344", "cog_category": "I", "kegg_orthology_id": "K08591", "go_ids": [ "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75428", "EC:2.3.1.15", "EC:2.3.1.275", "UniRef:UniRef50_P75428", "COG:COG0344", "COG:I", "KEGG:K08591", "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772" ] }, "pscc": { "uniref50_id": "UniRef50_P75428", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75428" ], "product": "Glycerol-3-phosphate acyltransferase" } }, "id": "CNOCLNGIAL_762", "locus": "GCA000143945_00736" }, { "type": "cds", "sequence": "contig_1", "start": 416236, "stop": 416445, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAAAACAAAGGCGCAAACCCAGTATTTAACTGAAGAACAAATGATCTTGGGTCCGCATTTGGTTAACTTTACTGATAAACAAATGTTAGTGAAATACTACAACGGCTTGCTAAAAAACTTTACAGCACGGTTAAATCCAAGCCAGTTTGATAGTCAAGTAATCCATGTATTAAATAAAATTATTAAAGCATATGAACGCAGCTAG", "aa": "MDKTKAQTQYLTEEQMILGPHLVNFTDKQMLVKYYNGLLKNFTARLNPSQFDSQVIHVLNKIIKAYERS", "aa_hexdigest": "5ba0c7c6b4b3478b5eb2ca5eacda8290", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8104.299299999999, "isoelectric_point": 9.347029304504396 }, "id": "CNOCLNGIAL_763", "locus": "GCA000143945_00737" }, { "type": "cds", "sequence": "contig_1", "start": 416503, "stop": 416895, "strand": "-", "frame": 3, "gene": "trmK", "product": "Putative tRNA methyltransferase MPN_351", "db_xrefs": [ "COG:COG2384", "COG:J", "EC:2.1.1.-", "GO:0005737", "GO:0008033", "GO:0032259", "GO:0160105", "KEGG:K06967", "SO:0001217", "UniRef:UniRef50_P47490", "UniRef:UniRef90_P75427" ], "nt": "ATGAAAAGAAGGATTAGCACAATTGCTAATTTAGTCTTACAGCAAAAACCCAAAGCCTTTTATGACATTGGTTGTGGCCATGGCTATTTAGCACGTGAACTAGTTCAACAAAACCCTAGTTTAGTGGGCGTTAACAGTGATATTAGTGCTAATGCATTAGGTGCTGCTAAAGGGTTGTTAAAAGATCACACTAACATGCACTTTATTACCAGTGACGGGTTTGACCTGTTACCTAACTTAAACTTAGATCCTGCTGTTGGTGTTATTGCTGGTTTAGGTGGGTTGAAAATTATGCAAATACTAGCACAGCACAATAACTTTTTAAAGCACTTTGTACTCCAACCGCAGTCCAACATCATTGAATTGCGCCGCTTTTTAAGTGCTAATCAATGA", "aa": "MKRRISTIANLVLQQKPKAFYDIGCGHGYLARELVQQNPSLVGVNSDISANALGAAKGLLKDHTNMHFITSDGFDLLPNLNLDPAVGVIAGLGGLKIMQILAQHNNFLKHFVLQPQSNIIELRRFLSANQ", "aa_hexdigest": "b26f3fce2ec0598e43722642478bc24b", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmK" ], "truncated": "5-prime", "pseudogene": { "start": 416236, "stop": 416895, "observations": { "mutation": [ 416505 ], "stop": [ 416505 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75427", "query_cov": 1.0, "subject_cov": 0.593607305936073, "identity": 1.0, "score": 258.0, "evalue": 4.69e-86, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKRRISTIANLVLQQKPKAFYDIGCGHGYLARELVQQNPSLVGVNSDISANALGAAKGLLKDHTNMHFITSDGFDLLPNLNLDPAVGVIAGLGGLKIMQILAQHNNFLKHFVLQPQSNIIELRRFLSANQWTITTETLVEERGFIYLVLAVDKTKAQTQYLTEEQMILGPHLVNFTDKQMLVKYYNGLLKNFTARLNPSQFDSQVIHVLNKIIKAYERS" }, "paralog": false, "identity": 0.9954337899543378, "score": 429.0, "evalue": 1.12e-155, "description": "Internal stop codon at 416505. Nonsense mutation around 416505.", "psc": { "uniref90_id": "UniRef90_P75427", "gene": "TrmK", "product": "Putative tRNA methyltransferase MPN_351", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47490", "cog_id": "COG2384", "cog_category": "J", "kegg_orthology_id": "K06967", "go_ids": [ "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75427", "EC:2.1.1.-", "UniRef:UniRef50_P47490", "COG:COG2384", "COG:J", "KEGG:K06967", "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_P47490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47490" ], "product": "Putative tRNA methyltransferase MG248" } }, "id": "CNOCLNGIAL_764", "locus": "GCA000143945_00738" }, { "type": "cds", "sequence": "contig_1", "start": 416879, "stop": 418378, "strand": "-", "frame": 2, "gene": "sigA", "product": "RNA polymerase sigma factor", "db_xrefs": [ "COG:COG0568", "COG:K", "GO:0003677", "GO:0005737", "GO:0006352", "GO:0016987", "RefSeq:WP_010874708.1", "SO:0001217", "UniParc:UPI0000134830", "UniRef:UniRef100_P78022", "UniRef:UniRef50_P78022", "UniRef:UniRef90_P78022" ], "nt": "ATGTCATCGCCAAAGAAAAATTTCAAAAAACCTCAGCCTAAAACTGAAAATCAAAAACAAGCACTTAACGAAGAGCGCATTGCTGAACTTAAAAAGAGTCGTATCCTCGGTAAAAACCGTCCGTTTAAAAAGATGATTTACGTTGACACTAAGGCACAACGTAAACAAAAACACGAAAACGTAGCCTTTTTAAAAACGCTTCAAGAAAACAAGGAGAGTGATGTACCAAAGAAGCGTCGCGGTCGAAAGCCAAAACACGCACCTTTAAAGGAAAAGAACAACCTTAAGCTGTTTGATATTTTAGAAGGCTCTCTAAAGAGTCATACCGAAAATGATGATACTAACAAGGTAATTAGCCTGTTGGTGGAGGTGTGGGAGAAAAAGAACAAAAAGAAAGACAATAACTCGCTTTCCAACAAGGACATTGTTAATGTTTTGTCCAAGTTTGAACTCCCAGATGATGAAATTATCTTTGTTTTGGATGAACTGCGTGACAAGGGAATTGAACTACCACATGATGTGGAAGAGCACATCCATGAGTTCCGTGCTAACCAAGACTTGTCGATCATTGACGAGGACATTGAAGAATTAACCACCAAAAACATCTCTAATCGTGATAAGGTTGATGACAACGTACGTTTCTTTCTAGGTTCGCTCGATTCTTCCAAGATGTTGGACTTTGAGTCTGAGCAGCGAATTGCCAAAGTTTTAAACAGTACTGATGAAGAATCACGCAAGTATGCCATTAATCAACTGGTAACCTCTAACCTTAGGTTAGTGGTCTCCATTGCTAAAAAACACTTGGAAAGAGGATTGGATTTTAATGACCTTATCCAGGAAGGTAACCTAGGACTTTTAAAGGCCATTTCCAAGTTTAACTGGTCATTGGGTAATAAGTTTTCCACTTATGCTACTTGGTGGATTAAACAAGCGATTACTCGTGCTATTGCTGATCAAGCACGTACGGTTCGGATTCCCGTACACATGGTGGAAACTATTAACCGTTTAGCTAAAGCTGAACGTGCCTTAAACCAAGAATTAGGACGCGAACCCACCGCGGAAGAGTTAGCGGAAAAAATGGGTGGTCAAGCTGAAGGGTTTACAGTTAAAAAGATTGCTGAAATTAAACGTTTAAGCTTAGATCCTGTTTCTTTAGATAAAACAGTGGGCCACGATGAAGAATCGCAGTTCGGTGACTTTGTGCGTGATACTGATGCCCAAATGCCCGATGAGTTTACCGAAAGCCGTTCTAATTACGAAAAGATTGATGAGTTGTTAAACAACTGTTTGTCCGAACAGGAAGAATTAATTGTGCGGATGCGCATTGGTATGCCACCTTACAACGAAACCAAAACACTCGATGAGGTAAGTCAAAAGATTAAGATTCCCCGCGAAAAGATTCGGCAAATAGAAACCAAAGCCATTCGCAAACTGCGTCAAGCAGTGCGTAATAACCACATGAGCCTTTCCTTCATGAGGGGAAATGAAAAGAAGGATTAG", "aa": "MSSPKKNFKKPQPKTENQKQALNEERIAELKKSRILGKNRPFKKMIYVDTKAQRKQKHENVAFLKTLQENKESDVPKKRRGRKPKHAPLKEKNNLKLFDILEGSLKSHTENDDTNKVISLLVEVWEKKNKKKDNNSLSNKDIVNVLSKFELPDDEIIFVLDELRDKGIELPHDVEEHIHEFRANQDLSIIDEDIEELTTKNISNRDKVDDNVRFFLGSLDSSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFNWSLGNKFSTYATWWIKQAITRAIADQARTVRIPVHMVETINRLAKAERALNQELGREPTAEELAEKMGGQAEGFTVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVRDTDAQMPDEFTESRSNYEKIDELLNNCLSEQEELIVRMRIGMPPYNETKTLDEVSQKIKIPREKIRQIETKAIRKLRQAVRNNHMSLSFMRGNEKKD", "aa_hexdigest": "8bec5144e8d4fcd777528620d647dbbf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134830", "ncbi_nrp_id": "WP_010874708.1", "uniref100_id": "UniRef100_P78022", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134830", "RefSeq:WP_010874708.1", "UniRef:UniRef100_P78022" ] }, "ips": { "uniref100_id": "UniRef100_P78022", "uniref90_id": "UniRef90_P78022" }, "psc": { "uniref90_id": "UniRef90_P78022", "gene": "sigA", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_P78022", "cog_id": "COG0568", "cog_category": "K", "go_ids": [ "GO:0005737", "GO:0003677", "GO:0016987", "GO:0006352" ] }, "pscc": { "uniref50_id": "UniRef50_P78022", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78022" ], "product": "RNA polymerase sigma factor SigA" }, "genes": [ "sigA" ], "id": "CNOCLNGIAL_765", "locus": "GCA000143945_00739" }, { "type": "cds", "sequence": "contig_1", "start": 418431, "stop": 418646, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAGCCTGATTGGGCTGTGTTTAACAGTATTTTAGGGGAACAACAAGCAATGTTTCCAACAACTTTTTTAGCGCAAATTAAGGAGTTCTTTTTAAACAAATCACTCTCATATGACCCTGAGGATTATGAGGAAGATTTACAGTTTTTTCGGCAACTAATTGTGAAACAAAAAGAGCTTTTAAAATATTTTAAAAGCATGGTTGAGCACTAA", "aa": "MQEPDWAVFNSILGEQQAMFPTTFLAQIKEFFLNKSLSYDPEDYEEDLQFFRQLIVKQKELLKYFKSMVEH", "aa_hexdigest": "86b33f35e0a08ef27e88393a2e9f8caa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8599.727399999998, "isoelectric_point": 4.637345695495605 }, "id": "CNOCLNGIAL_766", "locus": "GCA000143945_00740" }, { "type": "cds", "sequence": "contig_1", "start": 418821, "stop": 419222, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTAGCTGAAGGTAGTGATGCGGTTAAAGCTAAGACTCACAGGGTTTTAAACCTCGTGGAATACTTAGTGGCTTACTTTAAAACCAAGGGCTTTGATGAGCGGATTACCGTCAATAAAGTTATCGACATTATTGCACAAAACCAGAAAGTAACAGCTGACACTAGCTTTAGCCGCTTTTTGTGTCAGAAATTACAGCAATTGTTGCAATACAGTGATGTGGAAACACTGTTCACACAATTACAGCAGCAAAAGCTCAAAGTAAAAGTTAATAAAACTACAACTTTTACCCAAAGAGCCCCGATTTACGAATCAGTGGTTGGTGTGGTTGATAATAGTTTTAGAAATGAATCGCAGCCGGTTGCCATTACCAAGGAGTTTTTAGTCGAAAACAACTGA", "aa": "MFLAEGSDAVKAKTHRVLNLVEYLVAYFKTKGFDERITVNKVIDIIAQNQKVTADTSFSRFLCQKLQQLLQYSDVETLFTQLQQQKLKVKVNKTTTFTQRAPIYESVVGVVDNSFRNESQPVAITKEFLVENN", "aa_hexdigest": "8447be9a46f79bab4d6608b95f274484", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15239.282399999996, "isoelectric_point": 9.013985633850098 }, "id": "CNOCLNGIAL_767", "locus": "GCA000143945_00741" }, { "type": "cds", "sequence": "contig_1", "start": 419253, "stop": 419654, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08275.17", "PFAM:PF13662.12" ], "nt": "ATGATTCCGATCCATGACCTTAAGGGTAATCCAGTAGGTTTTTCAGGGCGTGCACTGCAAAAAACGGAAAAGATTAAATACAAAAACAGTGCAGAACACCAGTGGTTTAAGAAGAGTGAATTGTTATTTAACTTCCACCGGATTGATAAAAACACACTAAAGCTGTATTTAGTTGAGGGTTACTTCGATGTCTTTGCCCTTACTAGCGCTGGGATTGGTGATGTAGTGGGCTTAATGGGTTTAGCCTTGAGTGAAAGCCACATTATTGCCTTTCAACAGCAACTAAAAGCGCTAGAAACAGTGGTCTTGGCACTCGATAATGATACAGCGGGTCACGATGCTACTTTTAAGCTATTACAAGAGTTAAACGCACACGGCATTATTGTGGAAGTGGTCGATTGA", "aa": "MIPIHDLKGNPVGFSGRALQKTEKIKYKNSAEHQWFKKSELLFNFHRIDKNTLKLYLVEGYFDVFALTSAGIGDVVGLMGLALSESHIIAFQQQLKALETVVLALDNDTAGHDATFKLLQELNAHGIIVEVVD", "aa_hexdigest": "52237bdf68ef7cdbe2c613521183cc7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08275.17", "name": "DNAG_N", "length": 47, "aa_cov": 0.3533834586466165, "hmm_cov": 1.0, "evalue": 1.1792264885096112e-17, "score": 59.711673736572266, "start": 1, "stop": 47 }, { "id": "PF13662.12", "name": "Toprim_4", "length": 82, "aa_cov": 0.5864661654135338, "hmm_cov": 1.0, "evalue": 4.363694822352054e-16, "score": 54.42559051513672, "start": 55, "stop": 132 } ], "seq_stats": { "molecular_weight": 14807.884399999984, "isoelectric_point": 6.041268730163575 }, "id": "CNOCLNGIAL_768", "locus": "GCA000143945_00742" }, { "type": "cds", "sequence": "contig_1", "start": 420054, "stop": 420293, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01807.25" ], "nt": "ATGACCAGTCCCACTAGCCTAGATCAGCTCAAACAGCAAATCAAAATAGCTCCCATAGTGGAGCACTATGCCATTAAGCTAAAAAAGAAGGGGAAGGACTTTGTGGCACTCTGTCCCTTTCATGCTGACCAAAACCCCTCGATGACGGTGTCAGTTGCAAAGAACATCTTTAAGTGTTTCTCGTGTCAGGTGGGCGGTAATGGGATTGCTTTTATCCAAAAAATTGATCAGGTAGATTGA", "aa": "MTSPTSLDQLKQQIKIAPIVEHYAIKLKKKGKDFVALCPFHADQNPSMTVSVAKNIFKCFSCQVGGNGIAFIQKIDQVD", "aa_hexdigest": "892d3f54cfc6476dc5b8e05308af2bc7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01807.25", "name": "Zn_ribbon_DnaG", "length": 76, "aa_cov": 0.9620253164556962, "hmm_cov": 0.9868421052631579, "evalue": 2.581176845611923e-32, "score": 106.07622528076172, "start": 4, "stop": 79 } ], "seq_stats": { "molecular_weight": 8723.151799999998, "isoelectric_point": 9.125064659118653 }, "id": "CNOCLNGIAL_769", "locus": "GCA000143945_00743" }, { "type": "cds", "sequence": "contig_1", "start": 420484, "stop": 421215, "strand": "-", "frame": 3, "gene": "glyS", "product": "glycine--tRNA ligase", "db_xrefs": [ "COG:COG0423", "COG:J", "EC:6.1.1.14", "GO:0000166", "GO:0004820", "GO:0005524", "GO:0005737", "GO:0006426", "GO:0046983", "KEGG:K01880", "SO:0001217", "UniRef:UniRef50_P75425", "UniRef:UniRef90_P75425" ], "nt": "TTGTTCCAAACTGAAATTGGCGTTGTCAACAGCGAAAAACGCCTGGTTTACCTTCGTCCGGAAACAGCTCAGGGCATCTTCATTAACTTTAAACAGCTGTTACAACTCAAAAAGCGTCCTTTACCGTTTGGCGTAGCCCAGTTTGGTAAGAGCTTTCGCAATGAAGTGACCCCGGGAAACTTTTTGTTTCGCGTTCGTGAATTTGAACAGTTTGAAATGGAGTGGTTCTGCAATCCTCAAGCCAGTTTAAGCGTTTTTGAAAGCCAACAACAAGCAATTGCTCACTTTTTGTTTAAGGTACTGCAATTAAACCCAGCATTGGTTAAACAATACGAGTATGACAAGAATGAATTAGCACACTATGCCAATAAAACAGTGGATTTTTTATTCCAGTTTCCCCATGGCTTGAGGGAATTGTGGGGTTTAGCTGATCGTGGTACCTTTGATTTAGAACAACACCAAAAGTACGCCAAAAAGCCCTTAGACTTCTTTGATGGAGAGAATAACGAACACTTTATTCCAGCTGTAGTGGAGCCTTCGGTGGGAATTGAGCGCTTGTTTTATGCCTTGATTGTTAGCAGCTATCAACAAGAGCAGTTGGAAGGGGAAATGCGCGAAGTGTTACGTTTACCCTTTCACTTATGTCCAGAACAAATTGTAGTGCTGCCCTTAGTCAATAAACTCAAAGAAACAGCACAAACATTATTTGAAGCGCTGAGTCAAACCCACTGA", "aa": "MFQTEIGVVNSEKRLVYLRPETAQGIFINFKQLLQLKKRPLPFGVAQFGKSFRNEVTPGNFLFRVREFEQFEMEWFCNPQASLSVFESQQQAIAHFLFKVLQLNPALVKQYEYDKNELAHYANKTVDFLFQFPHGLRELWGLADRGTFDLEQHQKYAKKPLDFFDGENNEHFIPAVVEPSVGIERLFYALIVSSYQQEQLEGEMREVLRLPFHLCPEQIVVLPLVNKLKETAQTLFEALSQTH", "aa_hexdigest": "f9147e9b9cf98d319e9b7c8c23760b72", "start_type": "TTG", "rbs_motif": null, "genes": [ "glyS" ], "truncated": "both", "pseudogene": { "start": 420277, "stop": 421515, "observations": { "mutation": [ 420486, 421287, 421401 ], "stop": [ 420486, 421287, 421401 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75425", "query_cov": 1.0, "subject_cov": 0.5412026726057907, "identity": 0.996, "score": 493.0, "evalue": 1.95e-173, "gene_start": 1, "gene_end": 243, "reference_start": 138, "reference_end": 380, "reference_sequence": "MAQVYSQEVYVQYLKRYGFVFQSSELYNGLANSWDFGPLGAVLKQQIKTALYNFFIKNKRDVLLIDTPIILNEQIWKASGHLANFTDALVDCKSCKLRFRVDHLDEQIKSATQWNPKQVNCPNCKANNWSEVRDFNLLFQTEIGVVNSEKRLVYLRPETAQGIFINFKQLLQLKKRPLPFGVAQFGKSFRNEVTPGNFLFRVREFEQFEMEWFCNPQASLSVFESQQQAIAHFLFKVLQLNPALVKQYEYDKNELAHYANKTVDFLFQFPHGLRELWGLADRGTFDLEQHQKYAKKPLDFFDGENNEHFIPAVVEPSVGIERLFYALIVSSYQQEQLEGEMREVLRLPFHLCPEQIVVLPLVNKLKETAQTLFEALSQTHWRIGFESAGSIGKRYRKADAIGTKFAITFDFESLEDQAVTIRERDSLKQVRVPIKELKAWFAQHDDQSH" }, "paralog": false, "identity": 0.9927184466019418, "score": 821.0, "evalue": 5.94e-305, "description": "Internal stop codon at 420486, 421287, 421401. Nonsense mutation around 420486, 421287, 421401.", "psc": { "uniref90_id": "UniRef90_P75425", "gene": "glyS", "product": "glycine--tRNA ligase", "ec_ids": [ "6.1.1.14" ], "uniref50_id": "UniRef50_P75425", "cog_id": "COG0423", "cog_category": "J", "kegg_orthology_id": "K01880", "go_ids": [ "GO:0000166", "GO:0004820", "GO:0005524", "GO:0005737", "GO:0006426", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75425", "EC:6.1.1.14", "UniRef:UniRef50_P75425", "COG:COG0423", "COG:J", "KEGG:K01880", "GO:0000166", "GO:0004820", "GO:0005524", "GO:0005737", "GO:0006426", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P75425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75425" ], "product": "Glycine--tRNA ligase" } }, "id": "CNOCLNGIAL_770", "locus": "GCA000143945_00744" }, { "type": "cds", "sequence": "contig_1", "start": 421399, "stop": 421626, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCAAGTATATTCCCAAGAAGTGTATGTGCAATACCTCAAGCGCTATGGCTTTGTCTTTCAAAGTAGTGAGCTTTACAATGGACTTGCCAACAGCTGGGACTTCGGTCCGTTGGGTGCCGTTTTAAAGCAACAAATTAAAACGGCACTGTACAACTTCTTCATTAAAAATAAGCGGGATGTATTGTTGATTGACACGCCAATTATTCTCAACGAACAGATCTGA", "aa": "MAQVYSQEVYVQYLKRYGFVFQSSELYNGLANSWDFGPLGAVLKQQIKTALYNFFIKNKRDVLLIDTPIILNEQI", "aa_hexdigest": "8a04040f23482aca9578b1a785557d31", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8752.998099999997, "isoelectric_point": 7.961281394958497 }, "id": "CNOCLNGIAL_771", "locus": "GCA000143945_00745" }, { "type": "cds", "sequence": "contig_1", "start": 421867, "stop": 422376, "strand": "-", "frame": 3, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "EC:2.1.1.-", "GO:0003723", "GO:0005829", "GO:0006396", "GO:0008173", "GO:0032259", "SO:0001217", "UniRef:UniRef50_P47494", "UniRef:UniRef90_P75424" ], "nt": "ATGAAGCGGATGCAAGAAACCAGTTTTTTATTTGGTAGTAAGGCCTTTTTGGAAGCGTTAGATAACCAACTGCAAATTAAAAAGGTCAATTTATCTGATAAGCACCAAAAGCTGTTGGGCTTAATTAAGAAACGGGGATTGCGGTATGAAATGCACTCCAGCCAGTGGTTTCACCAGCAGTTTCGCCACATTAACCACCAGGAATTTGTTTGTGTAATCAACCCTAACCAAATGCTCAAAACAATTGAGCAGTTAATCCAAATTACAGACAGCAAGTCCACCAGTACCCTGGTAATGCTCGATGAGATCCAAGATCTGCATAACTTTGGCGCCATCTTGCGCACCTGTATGGCAGCTGAAGTGGATGGGATTATCTTTAAAAAGCATAACCAGGCGCCCATTAACAGTACCGTTATTAGAACCAGTATGGGAACTGTCTTTTACCAGAACTTAGTGCAGGTTACTAACCTCAGTTATGCCATTACTACCCTGCAAAAACACGGCTTTTGA", "aa": "MKRMQETSFLFGSKAFLEALDNQLQIKKVNLSDKHQKLLGLIKKRGLRYEMHSSQWFHQQFRHINHQEFVCVINPNQMLKTIEQLIQITDSKSTSTLVMLDEIQDLHNFGAILRTCMAAEVDGIIFKKHNQAPINSTVIRTSMGTVFYQNLVQVTNLSYAITTLQKHGF", "aa_hexdigest": "1d35decbdb7125c536a9cf03cabbbcfb", "start_type": "ATG", "rbs_motif": "AAAAA", "genes": [ "rlmB" ], "truncated": "5-prime", "pseudogene": { "start": 421648, "stop": 422376, "observations": { "mutation": [ 421659, 421869 ], "stop": [ 421659, 421869 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75424", "query_cov": 1.0, "subject_cov": 0.6983471074380165, "identity": 0.988, "score": 327.0, "evalue": 2.8e-112, "gene_start": 1, "gene_end": 169, "reference_start": 1, "reference_end": 169, "reference_sequence": "MKRMQETSFLFGSKAFLEALDNQLQIKKVNLSDKHQKLLGLIKKRGLRYEMHSSQWFHQQFRHINHQEFVCVINPNQMLKTIEQLIQITDSKSTSTLVMLHEIQDPHNFGAILRTCMAAEVDGIIFKKHNQAPINSTVIRTSMGTVFYQNLVQVTNLSYAITTLQKHGFWTVATTLDERLKPKDYRQVDFDKRILLVGNEDKGLNALLLKNADLKVKIPMNPKLNSLNVSVAVGIILFGWKS" }, "paralog": false, "identity": 0.9834710743801653, "score": 465.0, "evalue": 7.94e-169, "description": "Internal stop codon at 421659, 421869. Nonsense mutation around 421659, 421869.", "psc": { "uniref90_id": "UniRef90_P75424", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47494", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0006396", "GO:0008173", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75424", "EC:2.1.1.-", "UniRef:UniRef50_P47494", "COG:COG0566", "COG:J", "GO:0003723", "GO:0005829", "GO:0006396", "GO:0008173", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_P47494", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47494" ], "product": "Uncharacterized tRNA/rRNA methyltransferase MG252" } }, "id": "CNOCLNGIAL_772", "locus": "GCA000143945_00746" }, { "type": "cds", "sequence": "contig_1", "start": 422529, "stop": 422801, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09190.17" ], "nt": "TTGGATTTAAGCCAATCTTTAATAGAGCAAGCTTGCAGTGATATTAAAAGGATCCAAAAGGCAGTCAATGTGTGCCGCACTTGGTTTGTGTATTCTGAACAATCCGCCATTCCCGCGCCCAAACAGTTTGAACCGGTGTTTAAGGCACTTCTCAATAACCTCAACTTTGCTAATGCCATTACCCACATTTGGAAGTTAGTCAAACAAATTAACCATGATGTAAGCAAGCAAAACTTGAGCGGTTTAAAGGAACATTTAAGCCATTTGGAGTGA", "aa": "MDLSQSLIEQACSDIKRIQKAVNVCRTWFVYSEQSAIPAPKQFEPVFKALLNNLNFANAITHIWKLVKQINHDVSKQNLSGLKEHLSHLE", "aa_hexdigest": "3eff6f093267ab8f71ec489b00920ee5", "start_type": "TTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09190.17", "name": "DALR_2", "length": 45, "aa_cov": 0.4444444444444444, "hmm_cov": 1.0, "evalue": 4.083107981143549e-06, "score": 22.796756744384766, "start": 48, "stop": 87 } ], "seq_stats": { "molecular_weight": 10315.780699999996, "isoelectric_point": 8.664438056945801 }, "id": "CNOCLNGIAL_773", "locus": "GCA000143945_00747" }, { "type": "cds", "sequence": "contig_1", "start": 422832, "stop": 423086, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01406.26" ], "nt": "GTGGAGTGTGCCTTTTTAATTGATTACAGCTTTAAAGACCAGTTGACAATTCATGGTGGTGGGGTGGATTTGAAGTTTCCCCATCATGAAAACGAAAACGCAATGCATATGGCACTGTATGACAAACCATTAACGCAGCATTGGATGCACATTGGCCACTTAATGTTTGAAAACCAAAAGATGTCCAAGTCACTGCAAAACTTTCTTTTAGCAGTTGACTTTTTGACAATCCATGACTTTCGCATTTTGCGCTGA", "aa": "MECAFLIDYSFKDQLTIHGGGVDLKFPHHENENAMHMALYDKPLTQHWMHIGHLMFENQKMSKSLQNFLLAVDFLTIHDFRILR", "aa_hexdigest": "16460efba93f13186417598b445de777", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 84, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.2986531489555976e-36, "score": 121.94621276855469, "start": 1, "stop": 84 } ], "seq_stats": { "molecular_weight": 9957.436399999991, "isoelectric_point": 6.196439170837402 }, "id": "CNOCLNGIAL_774", "locus": "GCA000143945_00748" }, { "type": "cds", "sequence": "contig_1", "start": 423150, "stop": 423692, "strand": "-", "frame": 1, "gene": "cysS", "product": "cysteine--tRNA ligase", "db_xrefs": [ "COG:COG0215", "COG:J", "EC:6.1.1.16", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270", "KEGG:K01883", "SO:0001217", "UniRef:UniRef50_P47495", "UniRef:UniRef90_P75423" ], "nt": "ATGAACCAATTTGAACCTAAATTTACCCTCATTGACACGGTGAGCAATCAGAGCGTGGTATTAGAACAAAAGCAAATTAACATTTATCTGTGTGGACCGACTGTTTACAATGATTTGCACCTAGGCAATACCCGTCCCTTAATTGTGTTTGATGTGCTACAAAGGGTGTTGCAAGCAGCGCAGTACAAGGTGCAGTTTGTCCAAAACATCACTGATATTGACGACAAGATTATCAAGATCGCGCAGCAACAGGAAATTAGTGAAGCGCAGTTGTGCAAACAGCAAATCACCGCTTACAAAAGCTTGTTGAAAAAGCTCAACATTTTGCCGATTAAACACCTCCAAGTCACCGATAAAATTGACAAAATGCCCGGTTACATTGCCCGGTTAGTCCAAATGGGCTTTGCTTACGTTTCGCCTTTGGGCAACACGTACTTTAGTGTAAGCCAATTACCCCAGTATGGTATTTTAGCCAACCGTGTTGTGGAAACAATTGAGGATGAAGCTACTGACAAACGCAATAAACTGGACTTTGTTTTGTGA", "aa": "MNQFEPKFTLIDTVSNQSVVLEQKQINIYLCGPTVYNDLHLGNTRPLIVFDVLQRVLQAAQYKVQFVQNITDIDDKIIKIAQQQEISEAQLCKQQITAYKSLLKKLNILPIKHLQVTDKIDKMPGYIARLVQMGFAYVSPLGNTYFSVSQLPQYGILANRVVETIEDEATDKRNKLDFVL", "aa_hexdigest": "2aed8abd60af96646db94d4d2e6e8bb8", "start_type": "ATG", "rbs_motif": null, "genes": [ "cysS" ], "truncated": "5-prime", "pseudogene": { "start": 422847, "stop": 423692, "observations": { "mutation": [ 423092, 423113, 423125, 423152 ], "stop": [ 423092, 423113, 423125, 423152 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75423", "query_cov": 1.0, "subject_cov": 0.41189931350114417, "identity": 0.9890000000000001, "score": 347.0, "evalue": 5.14e-117, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MNQFEPKFTLIDTVSNQSVVLEQKQINIYLCGPTVYNDLHLGNTRPLIVFDVLQRVLQAAQYKVQFVQNITDIDDKIIKIAQQQEISEAQLCKQQITAYKSLLKKLNILPIKHLQVTDKIDKMPGYIARLVKKGFAYVSPLGNTYFSVSQLPQYGILANRVVETIEDEATDKRNKLDFVLWKQTTAGVKWNSPWGWGRPGWHVECAFLIDYSFKDQLTIHGGGVDLKFPHHENENAMHMALYDKPLTQHWMHIGHLMFENQKMSKSLQNFLLAVDFLTIHDFRILRWLFYQKHYYHPLDLSQSLIEQACSDIKRIQKAVNVCRTWFVYSEQSAIPAPKQFEPVFKALLNNLNFANAITHIWKLVKQINHDVSKQNLSGLKEHLSHLEWALNILGIGFKSIHTKLNVQLIKKWASLRKNGQLDKADEVRQKLIKKGLL" }, "paralog": false, "identity": 0.9786476868327402, "score": 551.0, "evalue": 8.21e-200, "description": "Internal stop codon at 423092, 423113, 423125, 423152. Nonsense mutation around 423092, 423113, 423125, 423152.", "psc": { "uniref90_id": "UniRef90_P75423", "gene": "cysS", "product": "cysteine--tRNA ligase", "ec_ids": [ "6.1.1.16" ], "uniref50_id": "UniRef50_P47495", "cog_id": "COG0215", "cog_category": "J", "kegg_orthology_id": "K01883", "go_ids": [ "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75423", "EC:6.1.1.16", "UniRef:UniRef50_P47495", "COG:COG0215", "COG:J", "KEGG:K01883", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47495", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47495" ], "product": "Cysteine--tRNA ligase" } }, "id": "CNOCLNGIAL_775", "locus": "GCA000143945_00749" }, { "type": "cds", "sequence": "contig_1", "start": 423679, "stop": 424926, "strand": "-", "frame": 3, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47496", "UniRef:UniRef90_P78021" ], "nt": "GTGTCCAAAGCGGTGGCTAGCTTTGATCGACCCGAAGCATTAATTGAGTACCTCGATCAGTTTGACCAAACACGTGATCAGTTGAACTTCCAAGTCGATGGCCTAGTGGTTAAACTAGATAACTTTCACTTTTACGACCAGTTAGGGTTTACTAGTAAGTTTCCCCGTTGGGCGTTGGCGTACAAGTTTAAACCGAAGTTTGTCCAAACCAAACTGCGTGGTGTCTTGGTAACAGTGGGACGCACTGGCAAGATTAACTATACCGCGCAGTTAGATCCAGTTAACTTAGAGGGGACAGTAGTATCAGCAGCCACACTGCATAACTTTGACTACATTAAGGAGAAGGACATTCGTTTAAACGATACAGTGATAATTTACAAAGCGGGGGAAATTATCCCTAAGGTATTGGAGGTGTGTTTACCGTTAAGAACAGCAGCGAATCAACCGATTCCCGAACAAACCCACTGTCCGGCATGTCAAGCCCCGTTAGTGCAGTATAAGGATGAGGTAGATCAGTACTGTACCAATGAACGTTGTGACCAGCGTAACCTTGAAGCGATTAATTACTTTGTGTCCAAAACGGCTATGGACATCCAGGGGCTAAGTATCCAGACCATTAGTAAGCTGTATGAAAATAACTTAGTGCGCAGCGTCGTTGATTTATACCACTTAAAGGAGCACAAAGCCGCGGTGTTAGCCTTGGAACTTAAAATTGGTGAAGTGCTGTTCAATAAACTAATCAGCAGCATCGAACAGTCCAAAACCAAGGGGATGGCACGCTTGTTAACTGGGCTTGGCATTAAACATGTGGGGCAAGTGTTAGCCAAAAGCTTAACGAAGCACTTTGAAAACATCGATGCCTTACAATCCGCCAGTATGGAAACACTGTTAGAACTCCCTGATGTGGGACCGACTGTGGCAGAATCGTTGTTTAACTGGTTTCACAATGACAATAACTTACAGCTGTTAGCTGCACTTAAAGCAGTTGGAGTGCAAATGGATGCTTTAAAGAGCAACACTAACTTTGACACCGCGAGCATTTACTTTCAAAAGAGTTTTGTTATTACTGGCAGCTTTCCAATTAGTCGCGATACCATTAAAAACTTGCTCGCCAATAAGTATGATTGTCGTTTTACAAATAACGTCACTAGTAAAGTTGATTTTGTGTTAGCTGGCATTAAGGCTACACCTCGTAAACTTGAACAAGCTAAAGCCCTCAATATTCCCATTATTAATGAACCAATTTGA", "aa": "MSKAVASFDRPEALIEYLDQFDQTRDQLNFQVDGLVVKLDNFHFYDQLGFTSKFPRWALAYKFKPKFVQTKLRGVLVTVGRTGKINYTAQLDPVNLEGTVVSAATLHNFDYIKEKDIRLNDTVIIYKAGEIIPKVLEVCLPLRTAANQPIPEQTHCPACQAPLVQYKDEVDQYCTNERCDQRNLEAINYFVSKTAMDIQGLSIQTISKLYENNLVRSVVDLYHLKEHKAAVLALELKIGEVLFNKLISSIEQSKTKGMARLLTGLGIKHVGQVLAKSLTKHFENIDALQSASMETLLELPDVGPTVAESLFNWFHNDNNLQLLAALKAVGVQMDALKSNTNFDTASIYFQKSFVITGSFPISRDTIKNLLANKYDCRFTNNVTSKVDFVLAGIKATPRKLEQAKALNIPIINEPI", "aa_hexdigest": "eda9af38580a1d031fa2d8301821c2ba", "start_type": "GTG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 423673, "stop": 425226, "observations": { "mutation": [ 423681, 424938 ], "stop": [ 423681, 424938 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78021", "query_cov": 1.0, "subject_cov": 0.6306990881458967, "identity": 0.993, "score": 702.0, "evalue": 8.33e-250, "gene_start": 1, "gene_end": 415, "reference_start": 242, "reference_end": 656, "reference_sequence": "MAKVAQIRAIVEQLKRYDYHYYVLDDPLVSDFEYDQLYKQLQALEQAHPELIQPDSPTQRVGGIVVEAFNKFPHQNPMLSLDNAFDTATIASFIANINNLTGVQNRFVVEPKIDGVSISLTYENGLLTRALTRGDGLVGEDVLSNVKTIKSIPLTIPFHKQVEIRGEIFVDKKTFAQINENLAKPFANARNLAAGTLRNLNSEVSAKRKLKAFFYFVPNALTLGCRSQSAVLAQLKQWNFPVSKAVASFDRPEALIEYLEQFDQTRDQLNFQVDGLVVKLDNFHFYDQLGFTSKFPRWALAYKFKPKFVQTKLRGVLVTVGRTGKINYTAQLDPVNLEGTVVSAATLHNFDYIKEKDIRLNDTVIIYKAGEIIPKVLEVCLPLRTAANQPIPEQTHCPACQAPLVQYKDEVDQYCTNERCDQRNLEAINYFVSKTAMDIQGLSIQTISKLYENNLVRSVVDLYHLKEHKAAVLALELKIGEVLFNKLISSIEQSKTKGMARLLTGLGIKHVGQVLAKSLTKHFENIDALQSASMETLLELPDVGPTVAESLFNWFHNDNNLQLLAALKAVGVQMDALKSNTNFDTASIYFQKSFVITGSFPISRDTIKNLLVNKYDCRFTNNVTSKVDFVLAGIKATPRKLEQAKALNIPIINEPIWT" }, "paralog": false, "identity": 0.9922630560928434, "score": 897.0, "evalue": 0.0, "description": "Internal stop codon at 423681, 424938. Nonsense mutation around 423681, 424938.", "psc": { "uniref90_id": "UniRef90_P78021", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_P47496", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78021", "EC:6.5.1.2", "UniRef:UniRef50_P47496", "COG:COG0272", "COG:L", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47496", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47496" ], "product": "DNA ligase" } }, "id": "CNOCLNGIAL_776", "locus": "GCA000143945_00750" }, { "type": "cds", "sequence": "contig_1", "start": 424936, "stop": 425649, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23", "PFAM:PF22745.1" ], "nt": "ATGGCAAAGGTCGCACAAATTCGAGCAATAGTTGAACAGTTAAAACGCTATGATTACCATTACTATGTTTTGGACGATCCGCTGGTCAGTGACTTTGAATACGACCAGTTATACAAACAGCTGCAAGCGTTGGAGCAAGCCCATCCTGAGTTAATCCAACCCGATTCACCAACCCAACGTGTTGGGGGAATTGTAGTGGAAGCATTTAATAAGTTTCCTCACCAAAATCCAATGCTTTCCCTAGACAATGCCTTCGACACAGCAACAATTGCTAGTTTTATTGCCAACATTAACAACTTAACTGGAGTGCAAAACCGCTTTGTGGTGGAACCCAAAATTGATGGTGTAAGCATCTCTTTAACTTACGAAAACGGATTGTTAACTCGGGCTTTAACCCGCGGTGATGGACTTGTGGGTGAGGATGTTTTGAGTAATGTTAAAACGATTAAGAGCATTCCCCTAACGATTCCTTTTCACAAACAGGTGGAAATCCGCGGTGAGATCTTTGTCGATAAAAAAACATTTGCGCAAATTAACGAGAATTTAGCAAAACCGTTTGCCAATGCCCGCAATTTAGCAGCAGGTACACTACGGAACCTCAACAGTGAGGTGAGTGCCAAGCGCAAACTTAAGGCCTTCTTTTACTTTGTTCCCAACGCCTTAACTTTGGGTTGTAGGAGTCAAAGTGCCGTGTTAGCACAGTTAAAGCAGTGA", "aa": "MAKVAQIRAIVEQLKRYDYHYYVLDDPLVSDFEYDQLYKQLQALEQAHPELIQPDSPTQRVGGIVVEAFNKFPHQNPMLSLDNAFDTATIASFIANINNLTGVQNRFVVEPKIDGVSISLTYENGLLTRALTRGDGLVGEDVLSNVKTIKSIPLTIPFHKQVEIRGEIFVDKKTFAQINENLAKPFANARNLAAGTLRNLNSEVSAKRKLKAFFYFVPNALTLGCRSQSAVLAQLKQ", "aa_hexdigest": "fa3048915e09a50043b520f9e07b07fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 181, "aa_cov": 0.7172995780590717, "hmm_cov": 1.0, "evalue": 4.540030507400302e-68, "score": 224.92138671875, "start": 68, "stop": 237 }, { "id": "PF22745.1", "name": "Nlig-Ia", "length": 37, "aa_cov": 0.15611814345991562, "hmm_cov": 1.0, "evalue": 1.768036892106903e-08, "score": 30.469568252563477, "start": 17, "stop": 53 } ], "seq_stats": { "molecular_weight": 26510.046300000016, "isoelectric_point": 8.543688774108887 }, "id": "CNOCLNGIAL_777", "locus": "GCA000143945_00751" }, { "type": "tRNA", "sequence": "contig_1", "start": 425713, "stop": 425786, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 78.9, "nt": "GCAGATATAGTTCAATGGCAGAACATAACCTTGCCAAGGTTAAGATGCGGGTTCGATTCCCGTTATCTGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 425752, 425754 ], "id": "CNOCLNGIAL_32", "locus": "GCA000143945_00752" }, { "type": "cds", "sequence": "contig_1", "start": 425817, "stop": 426107, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B2F78A2", "UniRef:UniRef90_UPI000B2F78A2" ], "nt": "ATGGAAACGCAAAACCAAATAGAAACGTTGCGCTATATTTTTAATCAGCTCAACAACCAAGACAAACCACAAATAATTTGGTTTTCCGGTGAAGGTGAGGATGAAAAAATTAACTTTTTAATCCGTTTGGACAATTATTTCCAACCTACCTTTGTCCAAGACCTTACTATTAACTTTCTCCCCGCTTTTGTTAAGCGAAACAAGAAAAATCCACCCAATACTTTAGCTAAGGGTAACTTTGTCAACATAGCTAATAAGTTATTGGCAGTGTTAGCGCGTTCCTTGAGCTGA", "aa": "METQNQIETLRYIFNQLNNQDKPQIIWFSGEGEDEKINFLIRLDNYFQPTFVQDLTINFLPAFVKRNKKNPPNTLAKGNFVNIANKLLAVLARSLS", "aa_hexdigest": "5b337a5ef0e9e2ad55eaf229c825958c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 425817, "stop": 426408, "observations": { "insertion": [ 426384 ], "mutation": [ 426105, 426255 ], "stop": [ 426105, 426255 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000B2F78A2", "query_cov": 1.0, "subject_cov": 0.48484848484848486, "identity": 1.0, "score": 192.0, "evalue": 8.67e-61, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "METQNQIETLRYIFNQLNNQDKPQIIWFSGEGEDEKINFLIRLDNYFQPTFVQDLTINFLPAFVKRNKKNPPNTLAKGNFVNIANKLLAVLARSLSWKQLNKPQQKWLLWLLVPFLLLRQLWLKKKVSKIFQFVNERGILSFIKEQWPILTTLVTVGTTLGTPIFSITISQQKAILENAGHGAFVFLVIFRCLQLRWV" }, "paralog": false, "identity": 0.9897959183673469, "score": 379.0, "evalue": 1.26e-136, "description": "Internal stop codon at 426105, 426255. Frameshift due to insertion around 426384. Nonsense mutation around 426105, 426255.", "psc": { "uniref90_id": "UniRef90_UPI000B2F78A2", "uniref50_id": "UniRef50_UPI000B2F78A2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000B2F78A2", "UniRef:UniRef50_UPI000B2F78A2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000B2F78A2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B2F78A2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11172.653899999981, "isoelectric_point": 9.098374748229983 }, "id": "CNOCLNGIAL_778", "locus": "GCA000143945_00753" }, { "type": "cds", "sequence": "contig_1", "start": 426282, "stop": 426974, "strand": "+", "frame": 3, "gene": null, "product": "putative MG255-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75422", "UniRef:UniRef90_P75422" ], "nt": "GTGGGTACTACCTTAGGTACACCCATCTTTTCGATTACCATTTCCCAGCAAAAAGCAATTTTGGAAAACGCTGGTCATGGTGCGTTTGTCTTTTTAGTAATCTTTTCGGTGTTTGCAATTGCGCTGGGTTTAGTCTCATCGTTAATCTTTTTAGTCTCATCGTTATTTTCAATTCGTCAAAAAAAGAGCTTACAACAGCTCCACCAAATTCTCTCTCGGTTAATTAACAAGTACTTCTGTTTGGCTAACAGTGAACAAAATCAAACCGGGCGGTACCAACTGAAAAATACCGGGGTGTGCTTCTTTTATGGCTTTGACTTTGAGGAAAAGGAATACATAACCCAAGCGATGAACCTACTGTTGCTCTTGAAGCAAACGAACTGCTTTGTGTTAGTCGGTTGTAAAGAGAGCAACATGTTGTTAATTAAAAACAAGGTGGAACCCGACATTAACCTTAAACAAAGTAGTCTGTACCTTGATTTAAAGAGTCAAATTAGTCCGTTGGCACAAATTAGTAAGTACAACCTCTTGTTTGAAGAGTTGGCCCTGGATGCTGACATGTTTTACTTAGAGGACTTCTTCGCCTTGTTAAAGACTCCGCGGCAAATTGTCAACTTTTTGTTTCGTATAAAACAAAACTTAAAGGAGTTTCACCAACCCCAAACGTTGTGGTTTGACTATTTGGCCTTGTGA", "aa": "MGTTLGTPIFSITISQQKAILENAGHGAFVFLVIFSVFAIALGLVSSLIFLVSSLFSIRQKKSLQQLHQILSRLINKYFCLANSEQNQTGRYQLKNTGVCFFYGFDFEEKEYITQAMNLLLLLKQTNCFVLVGCKESNMLLIKNKVEPDINLKQSSLYLDLKSQISPLAQISKYNLLFEELALDADMFYLEDFFALLKTPRQIVNFLFRIKQNLKEFHQPQTLWFDYLAL", "aa_hexdigest": "1580ff4d72680da9c7e4ae0feb568e8c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 425982, "stop": 427277, "observations": { "mutation": [ 426105, 426255, 426972 ], "stop": [ 426105, 426255, 426972 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75422", "query_cov": 1.0, "subject_cov": 0.4307116104868914, "identity": 0.991, "score": 434.0, "evalue": 4.82e-149, "gene_start": 1, "gene_end": 230, "reference_start": 156, "reference_end": 385, "reference_sequence": "METQNQIETLRYIFNQLNNQDKPQIIWFSGEGEDEKINFLIRLDNYFQPTFVQDLTINFLPAFVKRNKKNPPNTLAKGNFVNIANKLLAVLARSLSWKQLNKPQQKWLLWLLVPFLLLRQLWLKKKVSKIFQFVNERGILSFIKEQWPILTTLVTVGTTLGTPIFSITISQQKAILENAGHGAFVFLVIFSVFAIALGLVSSLIFLVSSLFSIRQKKSLQQLHQILSRLINKYFCFANSEQNQTGRYQLKNTGVCFFYGFDFEEKEYITQAMNLLLLLKQTNCFVLVGCKESNMLLIKNKVEPDINLKQSSLYLDLKSQISPLAQISKYNLLFEELALDADMFYLEDFFALLKTPRQIVNFLFRIKQNLKEFHQPQTLWFDYLALWALVIATDFEFNNVLWSFNDYLSLTNKQKEDYASVNLTAFFNRSLKNHKDNSLLFKPELFNTHAYIPETYTQVTLENIDSDKRAQLVPLNWFSQQKFSDFIEEKINFWQTQQAENKVFYLTLGERIFFLVLVNKKFKQIKLEAALKYLN" }, "paralog": false, "identity": 0.988399071925754, "score": 836.0, "evalue": 9.81e-310, "description": "Internal stop codon at 426105, 426255, 426972. Nonsense mutation around 426105, 426255, 426972.", "psc": { "uniref90_id": "UniRef90_P75422", "product": "Uncharacterized protein MG255 homolog", "uniref50_id": "UniRef50_P75422", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75422", "UniRef:UniRef50_P75422", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75422" ], "product": "Uncharacterized protein MG255 homolog" } }, "id": "CNOCLNGIAL_779", "locus": "GCA000143945_00754" }, { "type": "cds", "sequence": "contig_1", "start": 427014, "stop": 427295, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGGTCATTTAACGACTATTTAAGTTTAACGAACAAGCAAAAGGAAGATTATGCATCAGTTAACCTCACCGCCTTTTTTAACCGTAGTTTAAAGAACCATAAGGACAACAGCCTGCTCTTTAAACCAGAATTGTTCAACACTCACGCTTATATTCCTGAAACTTACACTCAAGTTACCTTAGAAAACATTGATTCAGACAAACGTGCGCAACTAGTGGCATTGAACTGGTTTTCACAGCAAAAGTTTAGCGATTTTATAGAAGAGAAAATTAACTTTTGA", "aa": "MWSFNDYLSLTNKQKEDYASVNLTAFFNRSLKNHKDNSLLFKPELFNTHAYIPETYTQVTLENIDSDKRAQLVALNWFSQQKFSDFIEEKINF", "aa_hexdigest": "02b2d9beeea0f06bd61d6cf547f58b5e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11092.25799999999, "isoelectric_point": 5.575132179260252 }, "id": "CNOCLNGIAL_780", "locus": "GCA000143945_00755" }, { "type": "cds", "sequence": "contig_1", "start": 427320, "stop": 427421, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTACCTCACTTTGGGCGAGCGGATCTTCTTTTTAGTATTAGTTAACAAGAAGTTCAAACAAATTAAACTAGAAGCAGCGTTGAAGTACCTCAACTAA", "aa": "MFYLTLGERIFFLVLVNKKFKQIKLEAALKYLN", "aa_hexdigest": "80607b1e5c74462264a515b250e1e760", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3992.853900000001, "isoelectric_point": 9.873542594909669 }, "id": "CNOCLNGIAL_781", "locus": "GCA000143945_00756" }, { "type": "tRNA", "sequence": "contig_1", "start": 427422, "stop": 427496, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 55.1, "nt": "CTTCCCTTGGTGTAATGGATAACACGACTGAGTCCTAATCAGTAAATGGAGGTTCGATTCCTCTAGGGAAGGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 427426, 427428 ], "id": "CNOCLNGIAL_10", "locus": "GCA000143945_00757" }, { "type": "tRNA", "sequence": "contig_1", "start": 427570, "stop": 427643, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 74.2, "nt": "GGGGGTGTAGTTTAGTGGCAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 427602, 427604 ], "id": "CNOCLNGIAL_11", "locus": "GCA000143945_00758" }, { "type": "cds", "sequence": "contig_1", "start": 427651, "stop": 428043, "strand": "+", "frame": 1, "gene": null, "product": "putative MG256-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47498", "UniRef:UniRef90_P75421" ], "nt": "ATGCATTACAGTTACAGCTTTAAAGAATACATCGAACGGTTTGCCAAAAAGGTTAATTCGATTGACAGCGAGTACTTCGAATTTTCCTCTTACATAGAGCGCATGCGCACTGTGTTTGGTTTACTGATTGCACTGATATGCTTTAGTAACGTTCTCTGTTTTCTTTTTATTGCAACTTGGTTTAGCACAAAGGGATTTGGTCAACACTACCGGGCGTTAATATTTACCTTGTTTATTCCCTTTGTCACTTCACTTTTAGCCAACATTATCTTCATTAACCTTAATCGCGCTTTTCGCGAATACTTTAAGATCAGCAGTAAAAGCCGTTCTTTTTTGGTAATCTGTGCCTTCTCCTCATTACCGATTGTGAACATCTGGCTAATGTTGTGGTGA", "aa": "MHYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLW", "aa_hexdigest": "5be2940fbd06b38a856224268460776e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 427651, "stop": 428346, "observations": { "mutation": [ 428041 ], "stop": [ 428041 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75421", "query_cov": 1.0, "subject_cov": 0.5038759689922481, "identity": 0.992, "score": 247.0, "evalue": 4.38e-81, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MNYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLWWVAMIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT" }, "paralog": false, "identity": 0.9913419913419913, "score": 463.0, "evalue": 1.84e-168, "description": "Internal stop codon at 428041. Nonsense mutation around 428041.", "psc": { "uniref90_id": "UniRef90_P75421", "product": "Uncharacterized protein MG256 homolog", "uniref50_id": "UniRef50_P47498", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75421", "UniRef:UniRef50_P47498", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47498", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47498" ], "product": "Uncharacterized protein MG256" } }, "id": "CNOCLNGIAL_782", "locus": "GCA000143945_00759" }, { "type": "cds", "sequence": "contig_1", "start": 428050, "stop": 428427, "strand": "+", "frame": 1, "gene": null, "product": "putative MG256-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47498", "UniRef:UniRef90_P75421" ], "nt": "ATGATTAAGCGCTTTACTAGTAACTATGCCTTTGCTATCTTTGATAAGTACAACGGTTTGACTAGTGGTGTGTTTATCTTTGACTTTGCTGATAATGTCAATTTTGAGGGTAAACTAGTGAGCTTTGACAATACCAAGGACACCAACCGTGACTTTGTCCACTTTTACAGTGAGGCCAAACTTAAACGCGATAAAATTACGCTACAAACGAATCCAATTCCACACGAAAGGATGTATGTTAACCGCATGTACTATCAGCAAAAGCTCAGTATGGGTGCTAACCAAAACAGCCCTTCAACGGCATTTGCTAACTTAAAGCGGTATGTGGAACATAAACAGCAAAAAATTATCAAGATTAAGCAGTTTATCTTGACCTAA", "aa": "MIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT", "aa_hexdigest": "96c3cce8e61a487fbe8823ac25ab6f0f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 427750, "stop": 428427, "observations": { "mutation": [ 428041 ], "stop": [ 428041 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75421", "query_cov": 1.0, "subject_cov": 0.4844961240310077, "identity": 1.0, "score": 247.0, "evalue": 5.05e-81, "gene_start": 1, "gene_end": 125, "reference_start": 134, "reference_end": 258, "reference_sequence": "MNYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLWWVAMIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT" }, "paralog": false, "identity": 0.9955555555555555, "score": 450.0, "evalue": 1.6e-163, "description": "Internal stop codon at 428041. Nonsense mutation around 428041.", "psc": { "uniref90_id": "UniRef90_P75421", "product": "Uncharacterized protein MG256 homolog", "uniref50_id": "UniRef50_P47498", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75421", "UniRef:UniRef50_P47498", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47498", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47498" ], "product": "Uncharacterized protein MG256" } }, "id": "CNOCLNGIAL_783", "locus": "GCA000143945_00760" }, { "type": "cds", "sequence": "contig_1", "start": 428472, "stop": 428765, "strand": "+", "frame": 3, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874716.1", "SO:0001217", "UniParc:UPI0000133F18", "UniRef:UniRef100_P78020", "UniRef:UniRef50_P47499", "UniRef:UniRef90_P78020" ], "nt": "ATGAAAAAAGATTTTCATTTTCCAAGCCAATCCGTTAGCTTCAAGTGTGCTTCTTGCAGCAATAGTTTCACCATTGAATCAACCCTAAAACAAAAAGAAATTACGATTGATATTTGTGGTAAATGTCACCCGTTTTACATTGGTGAATTAACCAAACAAACAGTCCACGGTCGTGCGGAAAAGCTTTCTGGAAAGTTCAATGCTGGCAAAGCCTTTTTAGAGAATAAAACTCCTAAAAAAGCGAAAGGCAAAACAGAAGAATATACCAAACACCGTTCTTTAAACGAACTGTAG", "aa": "MKKDFHFPSQSVSFKCASCSNSFTIESTLKQKEITIDICGKCHPFYIGELTKQTVHGRAEKLSGKFNAGKAFLENKTPKKAKGKTEEYTKHRSLNEL", "aa_hexdigest": "3c44c7a45b1bd5b7f6d5e80e4c2c5463", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133F18", "ncbi_nrp_id": "WP_010874716.1", "uniref100_id": "UniRef100_P78020", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133F18", "RefSeq:WP_010874716.1", "UniRef:UniRef100_P78020" ] }, "ips": { "uniref100_id": "UniRef100_P78020", "uniref90_id": "UniRef90_P78020" }, "psc": { "uniref90_id": "UniRef90_P78020", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_P47499", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47499" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "CNOCLNGIAL_784", "locus": "GCA000143945_00761" }, { "type": "cds", "sequence": "contig_1", "start": 428766, "stop": 429206, "strand": "+", "frame": 3, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_P47500", "UniRef:UniRef90_P75420" ], "nt": "GTGGAGTTTGACAAGCAGTTCTTTTCTAGTGTTGAAAAGATTGTTGAACTTGCTGAACAACTCGAAAAGGACTTAAATAAACCCGATTTGACCTTTGAACAGATCAAAGCGATCAACAAGGAACTGAAACATAAACAACCCTTAGTTGTTAAATTCAAAGAATTTAAACGGCTCATAGATCAAGCTTTGGAAGCGGAGGCCATTTTGGAAAACAACGAGCTCAAGGAGCTACATGATGAGGCCAAAAAAGAGCTGGAACGGGTGCGCAGTGTTGTCCCTGAATACGAAGAAGCGTTAAAACTGTTGTTATTACCAATTGATGAGAATAACCAGAAAAACGTGATTGTAGAGCTTCGTCCCGCTGCTGGTGGGGATGAATCGTGCATCTTTTTAGCTGATTTATTCAATATGTACCGCAACTTTTGTACTAACAAGGGTTGA", "aa": "MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLIDQALEAEAILENNELKELHDEAKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRPAAGGDESCIFLADLFNMYRNFCTNKG", "aa_hexdigest": "c0c64d44ddf7ae8abd90fcd93b1ef3eb", "start_type": "GTG", "rbs_motif": null, "genes": [ "prfA" ], "truncated": "3-prime", "pseudogene": { "start": 428766, "stop": 429509, "observations": { "mutation": [ 429204 ], "stop": [ 429204 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75420", "query_cov": 1.0, "subject_cov": 0.40668523676880225, "identity": 0.993, "score": 272.0, "evalue": 2.81e-89, "gene_start": 1, "gene_end": 146, "reference_start": 1, "reference_end": 146, "reference_sequence": "MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLIDQALEAEAILENNELKELHDEAKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRPAAGGDESCIFLADLFNMYRNFCSNKGWKLQINEMIPSSVGLNFVSFEVNGVDVFAKLKFESGVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNRTESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGSGDRAEKIRTYNYPQNRVTDHRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS" }, "paralog": false, "identity": 0.9878542510121457, "score": 475.0, "evalue": 1.75e-171, "description": "Internal stop codon at 429204. Nonsense mutation around 429204.", "psc": { "uniref90_id": "UniRef90_P75420", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_P47500", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75420", "UniRef:UniRef50_P47500", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_P47500", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47500" ], "product": "Peptide chain release factor 1" } }, "id": "CNOCLNGIAL_785", "locus": "GCA000143945_00762" }, { "type": "cds", "sequence": "contig_1", "start": 429225, "stop": 429845, "strand": "+", "frame": 3, "gene": null, "product": "Peptide chain release factor 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1W9TI19" ], "nt": "ATGATCCCATCCAGTGTCGGGTTAAACTTCGTTTCCTTTGAAGTTAATGGTGTCGATGTGTTTGCCAAGCTCAAGTTTGAATCTGGCGTACACCGGGTACAACGCGTACCCGCTACTGAATCCAAGGGGAGAGTACACACTTCAACCGTTACGGTTGCTGTTTTACCACAACTAGAAGCGGTTGAAGTTCATATTAACCCAGCTGATCTTAGAGTAGATACCTACCGTGCTTCGGGTGCTGGGGGTCAACACGTTAACCGGACTGAAAGTGCAGTGCGGATTACCCATTTACCAACTGGCATAGTAGTTTCGTGTCAAGAGGGTAAATCACAATTTACTAACCGTGATACGGCAATGAAGATGCTACGTGCTAAACTGTGGGAAAAAGCGCAGAACGAACAGTTATCAACCCAAGCAGGTTTGCGTAAAAGTCAGGTTGGGTCGGGTGATCGTGCCGAAAAGATCCGCACATATAACTATCCCCAAAACCGGGTCACAGACCACCGCATTAAACTTACCGTCAATAAGCTTAACACGATTATTTTAGGTGACCTTGATGAAATTATTGAAGCGTTGCAGGCAGATGAAAAGAAACAACAACTAGAGAACTTTTTCAGTTAA", "aa": "MIPSSVGLNFVSFEVNGVDVFAKLKFESGVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNRTESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGSGDRAEKIRTYNYPQNRVTDHRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS", "aa_hexdigest": "76d49743f1eca11ec3aa76929feb9462", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1W9TI19", "query_cov": 0.9660194174757282, "subject_cov": 0.8223140495867769, "identity": 0.643, "score": 248.0, "evalue": 1.85e-80, "valid": false, "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A0A1W9TI19", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0006415" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1W9TI19", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1W9TI19" ], "product": "Peptide chain release factor 1" }, "genes": [], "id": "CNOCLNGIAL_786", "locus": "GCA000143945_00763" }, { "type": "cds", "sequence": "contig_1", "start": 429845, "stop": 429970, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTGTATGAACTTTTCTTGAACCAAAAGCTGCTGTATGGCACCGATCCGCACTTTAACGGTGTTTTTCTCACCCTATTAGAAAAGTTTGGTCTGCACTTTAAGGATTTAACAGCACTGTGA", "aa": "MNLYELFLNQKLLYGTDPHFNGVFLTLLEKFGLHFKDLTAL", "aa_hexdigest": "f75fee177eed71695c8a8abee943c0ea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4815.5853, "isoelectric_point": 5.977779579162598 }, "id": "CNOCLNGIAL_787", "locus": "GCA000143945_00764" }, { "type": "cds", "sequence": "contig_1", "start": 430457, "stop": 430882, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTGATGGTAACGGAATTTTGTTTTACCAAAAGCTGTATAACTTTTTATTGGGTAACCGCCAAGTTAAACAAGTGATACTGGAGTGCTCACCAACCCAGAAAAAAGAGTTTTTAGCATTATTTAGCATTTTTAAAACTAGTGAAATTTACACAAGCCACAAGCAATTCATCGGTTTGAGTATTGACAACACTAAACTACCAGTCTTAAAAATTGCGCAAACCAAGCAAATTAAAGCACTGCTAGACAAGGGGATGACGGCCATTATCCCCACCGACACGCAAATAGGATTAATGAGTTATTGCCAACAAGATTTAGACCACATTAAACAACGCGATCCCAACAAGCACTACGTCCAGTTTTTAGCACCTAGTCAAATTAACCAGTTACCCAAACAATTACAAAAGCTAGCCAAACTGTTTTGA", "aa": "MADGNGILFYQKLYNFLLGNRQVKQVILECSPTQKKEFLALFSIFKTSEIYTSHKQFIGLSIDNTKLPVLKIAQTKQIKALLDKGMTAIIPTDTQIGLMSYCQQDLDHIKQRDPNKHYVQFLAPSQINQLPKQLQKLAKLF", "aa_hexdigest": "6a44a0a2d0cf2713a58c0cbc4ca36782", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16171.81199999998, "isoelectric_point": 9.543722343444824 }, "id": "CNOCLNGIAL_788", "locus": "GCA000143945_00765" }, { "type": "cds", "sequence": "contig_1", "start": 431051, "stop": 431206, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGCAGAACTGTTTTATTGTGCAAAACAGCTTTAAAAGTAATAACGAACCGTCCTTAATTTACAACTTGGATACGAAGCAAATTGTTCGTGGTAGTTCAACCCAATTACAACGTTTCCAAGCGTTACTAGCAAAACACAAATTAAGACACTAA", "aa": "MQQNCFIVQNSFKSNNEPSLIYNLDTKQIVRGSSTQLQRFQALLAKHKLRH", "aa_hexdigest": "5629f11c7c49c8c20d46cdc6852e5a84", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5974.7895, "isoelectric_point": 10.182410621643069 }, "id": "CNOCLNGIAL_789", "locus": "GCA000143945_00766" }, { "type": "cds", "sequence": "contig_1", "start": 431298, "stop": 431972, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTGTATAATTTCCCATCTTTTTTGGTGTTTTGTAAGATGAAATTTAAGTATGGTGCTACCCTTTTTAGTGGCTTTTTAGGGCTTTCAGCGATCCTAGCTGCTTGTGGTGCAAAGGGTAAATTTGACCAAGTTGATGATGGCAAAATTGTGTTAGCCTCTTCATTAACTTCTAAAAATGCCGCTAATGCTCTACAAGCTGTGGTGGAAAAATACAACCAGGTTAAGGGTGGCAATGATTATCCTATTGAAATTACCCAAATTACCGGTGGTTATGACGGTGGACGTGGTAACTTACAAACCAAACTTAGCGTTAAAGACAAAACGACCTTTTACAACCTCATCTTAAACTATCCCGACTTAGTTTCCACCCTAGGACGTGTTGGGATGGAACTGCCACTAGACAACGTTGATCTCTCCAAGTTATCACCTAACTTTTTGAAGTTCAACGAACGCATTAGCGGTGTAGCCAAAAAGGCCAATTACGCCATTCCCGTATCACTTTCAACTGAAGTTTTAGCTTTAAATGGACCGGTATTACACTATATCTTGAACAGCGCGAAGGGTGGTAATGAAACAGCTGTAACACAAAGAACTGCTAGCACAAACCAACAAAATACACTTCAAAATTCGTTAAAAATTAACGAAAGCGACCCCATAACCAAAGAGCTTTGA", "aa": "MLYNFPSFLVFCKMKFKYGATLFSGFLGLSAILAACGAKGKFDQVDDGKIVLASSLTSKNAANALQAVVEKYNQVKGGNDYPIEITQITGGYDGGRGNLQTKLSVKDKTTFYNLILNYPDLVSTLGRVGMELPLDNVDLSKLSPNFLKFNERISGVAKKANYAIPVSLSTEVLALNGPVLHYILNSAKGGNETAVTQRTASTNQQNTLQNSLKINESDPITKEL", "aa_hexdigest": "9817deb53f983d82c721abb7966fe543", "start_type": "TTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24226.444500000023, "isoelectric_point": 9.067300987243652 }, "id": "CNOCLNGIAL_790", "locus": "GCA000143945_00767" }, { "type": "cds", "sequence": "contig_1", "start": 432165, "stop": 433718, "strand": "+", "frame": 3, "gene": null, "product": "P80 family lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B" ], "nt": "GTGGAGAATGGCCTTAAGGGTTATACCTTTAAAGCTAGTGTCTTTGAAAACTGGCATGAACTGCTCGATTTTTCCGCAAGGATAGCTAGATCATTTACTAATATTCACACCAAAGAAACCAAAAAGGGAACGGACATTCAAGGTGTACTTGGGATTGACAGTACACCTAACGCCTTATTTACTTCAGTATTTGCTGCAGGTGGTGGTAACTATGACAACTTCTTTTATAAGGTGCAAGATGGTCGGGCTGACTTTAGTAACTTTAAAAATAAGGAAACCTCTTACCAAAACTTACAAAAAGTATTTAGCGATTATAAGGAGTTAATTGATAAAAACGGTTTGTTTGTCAATAAGGGTGGCTCTTACTCTTCTAACTTCCAGAAGTTCCACCAATTAGCTTACAGTATTTCTTCCACTTCAGGTTTCTTTTATTCGTTTGCTGGTAACAGTGCTAAACGCTTAAAGTTTGATGACGGTAACTTTATTGAGTTTCCGCGCTTCACTCAACCAGTTAAAGCGCCTAATAAAACAACTCAAAGTGGTGGAGAAAATAACAATAAAAGTGAAAATAATCTTCTTGGAACTTTTGAAATAGAAGACAAAAGCAAAAAGCGCGAAAGCGTTGAATCTAGTGAAAAATCAAGTTCTGGGACTCAATCCAAAAGCAAGAAAGGTAAAAGTATGAAAACCATTTACCTTTATAAAGCACAAATTCCAGAAGGAAAAAACAAAGGTGATAATGCCATCTTAATTACTAACCAAACTGTTATTGATAAACTAGAAAAAGCTGCTAAAGAAGACGGTAAAAGTGGAACTCAAGTTTCAACTGTTGCAAGTAAATCAACAACTAAAGAGAACCAAGAAAACAAAATAATTGGTTACACATCGACTGATAACCTTCGTGAAGACAATAAGAACATTTTCGATATCGATGATCTTAACGATGAAAAATATGACCGTAAGATTATTGTTAGTGCTACTGAAGAAGTATTGGAACAATCTAACACTTTACAGAGTGAAGAAGCAGTTGTTCTACCGGCACTTGCTAAATATAAAGCTAGTGATTCCACAAAAATAGCAATCACTCAAGAACCTAACCTAATTGGTATCCATGCTAACGAAAAGGAAAATGAGGAAACACGTAAGTTTGTTAATTGGTTTTTAAACAGTGAGCAAACTTGGGAATCCAAGAAATACAGTAAAAATAAGCAAAACTCCAACGAAAAGAGAACTCCTGCACAATTCTTTGCTGATTCCGCGTCTTACATTCTACCCTTAAAGAAAACCTTCGATAATGCCAACAAAAAAGAAGATCAAAGTAAGGCCAATAACAAGACTAATAGCCAAAGAACTAACACCTATATCGAAAAGGCATTAGAAATTTTTAAAGGAATTAGCCAAAACCAAATAACAAGTTACAGTGATCCAAGCGACTTTAGAAGTGGTCGCTTCCGCGATGGTATTGGTTCTAACTTTAATGCAGTAGTAAATTCCAAAGTGGATTTCAATAAATTTATTAATAACTTTATTGCTAGTCTAGGTTTTGACATCTAA", "aa": "MENGLKGYTFKASVFENWHELLDFSARIARSFTNIHTKETKKGTDIQGVLGIDSTPNALFTSVFAAGGGNYDNFFYKVQDGRADFSNFKNKETSYQNLQKVFSDYKELIDKNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFDDGNFIEFPRFTQPVKAPNKTTQSGGENNNKSENNLLGTFEIEDKSKKRESVESSEKSSSGTQSKSKKGKSMKTIYLYKAQIPEGKNKGDNAILITNQTVIDKLEKAAKEDGKSGTQVSTVASKSTTKENQENKIIGYTSTDNLREDNKNIFDIDDLNDEKYDRKIIVSATEEVLEQSNTLQSEEAVVLPALAKYKASDSTKIAITQEPNLIGIHANEKENEETRKFVNWFLNSEQTWESKKYSKNKQNSNEKRTPAQFFADSASYILPLKKTFDNANKKEDQSKANNKTNSQRTNTYIEKALEIFKGISQNQITSYSDPSDFRSGRFRDGIGSNFNAVVNSKVDFNKFINNFIASLGFDI", "aa_hexdigest": "f1ff6e0499b691aee0c09b4712adf5f2", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001330F80B", "query_cov": 0.8588007736943907, "subject_cov": 0.9466950959488273, "identity": 0.586, "score": 468.0, "evalue": 9.43e-159, "valid": false, "product": "P80 family lipoprotein", "uniref50_id": "UniRef50_UPI001330F80B" }, "pscc": { "uniref50_id": "UniRef50_UPI001330F80B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B" ], "product": "P80 family lipoprotein" }, "genes": [], "id": "CNOCLNGIAL_791", "locus": "GCA000143945_00768" }, { "type": "cds", "sequence": "contig_1", "start": 433756, "stop": 433935, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGAGATTTCTAGGGGCAAAGCAATTACCAAAGAATACATAAGAGCTAACCCTGGTGAATATCCTGTTTATTCTGGTTCAACATTAAATGATGGTGAAATAGGTCGCATAGATGAATGCGAATTTGATGGTGAATATGTAACTTGA", "aa": "MEIKTYKIKDICEISRGKAITKEYIRANPGEYPVYSGSTLNDGEIGRIDECEFDGEYVT", "aa_hexdigest": "6dc7da85cb8446a93bd914f3b9f7c3ac", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 61, "aa_cov": 0.9661016949152542, "hmm_cov": 0.9836065573770492, "evalue": 2.8403231306768355e-11, "score": 39.35646438598633, "start": 3, "stop": 59 } ], "seq_stats": { "molecular_weight": 6710.4442, "isoelectric_point": 4.644507408142091 }, "id": "CNOCLNGIAL_792", "locus": "GCA000143945_00769" }, { "type": "cds", "sequence": "contig_1", "start": 434062, "stop": 434475, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGGAAGCGCCTAAACATGTTAATAATGCTTGTGTTATTCCTAATCTAACTTTAAAAAAGATGAGAGAAATAGAACTAGATTTTCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATAACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGA", "aa": "MEAPKHVNNACVIPNLTLKKMREIELDFPSKKIQEKIATILDTFTELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYIT", "aa_hexdigest": "76b6e8516d96bbb35b19364825e78b37", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.4233576642335766, "hmm_cov": 0.9838709677419355, "evalue": 4.353872313435118e-21, "score": 71.28997039794922, "start": 80, "stop": 137 } ], "seq_stats": { "molecular_weight": 15910.972899999979, "isoelectric_point": 7.65106258392334 }, "id": "CNOCLNGIAL_793", "locus": "GCA000143945_00770" }, { "type": "cds", "sequence": "contig_1", "start": 434861, "stop": 434983, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004EC6A", "UniRef:UniRef100_A0AB38W7C9", "UniRef:UniRef50_A0AB38W7C9", "UniRef:UniRef90_A0AB38W7C9" ], "nt": "ATGGGCGGCCCGCACGACATCCAGCCCCCCATTGAGCGGTGTGGTAAAGGGCGTGTAGGGGGTGAGGGTGTTGTGGTCTTGGAGGAGGTAGGTGTTGGAAACTATTTATTAAGCATTACATAA", "aa": "MGGPHDIQPPIERCGKGRVGGEGVVVLEEVGVGNYLLSIT", "aa_hexdigest": "0783f67634c017e7377b3fa28055f056", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001004EC6A", "uniref100_id": "UniRef100_A0AB38W7C9", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004EC6A", "UniRef:UniRef100_A0AB38W7C9" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7C9", "uniref90_id": "UniRef90_A0AB38W7C9" }, "psc": { "uniref90_id": "UniRef90_A0AB38W7C9", "uniref50_id": "UniRef50_A0AB38W7C9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7C9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7C9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4133.705699999999, "isoelectric_point": 4.957690238952636 }, "id": "CNOCLNGIAL_794", "locus": "GCA000143945_00771" }, { "type": "cds", "sequence": "contig_1", "start": 435020, "stop": 435580, "strand": "+", "frame": 2, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_P75415" ], "nt": "GTGGGACAGAACAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGACTCTAATAACCAAGATACGAGTCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTAGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTATACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAACAACCCCGCTCCCAAGGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGA", "aa": "MGQNKLVALVRSALVRVKFQDTTSSDSNNQDTSQNALSFDTQESQKALNGSQSGSSDTSGSNSQDLASYILIFQAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLQDLLVEQPVTPYTPNAGLARVNGVAQDTVHFGSGQESSWNSQRSQKGLKTTPLPRPSPALSSIRAARTGS", "aa_hexdigest": "2941ccc9fcf17790f3040ad278ca3ebf", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 434954, "stop": 435769, "observations": { "mutation": [ 435011, 435578 ], "stop": [ 435011, 435578 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75415", "query_cov": 1.0, "subject_cov": 0.6838235294117647, "identity": 0.9840000000000001, "score": 345.0, "evalue": 1.57e-118, "gene_start": 1, "gene_end": 186, "reference_start": 24, "reference_end": 209, "reference_sequence": "MGGLDVVRAAHLHPSYELVDWKRVGQNKLVALVRSALVRVKFQDTTSSDSNNQDTSQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLQDLLVEQPVTPYTPNAGLARVNGVAQDTVHFGSGQESSWNSQRSQKGLKTTPLPMPSPALSSIRAARTGSWMKVDRCMNPWIRPRRGRGRMRALGKIRKKQRRKMMPRWWGWLEVVRLEVLLVYKAMARTVRG" }, "paralog": false, "identity": 0.985239852398524, "score": 529.0, "evalue": 1.73e-193, "description": "Internal stop codon at 435011, 435578. Nonsense mutation around 435011, 435578.", "psc": { "uniref90_id": "UniRef90_P75415", "product": "Putative MgpC-like protein MPN_366", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75415", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "CNOCLNGIAL_795", "locus": "GCA000143945_00772" }, { "type": "cds", "sequence": "contig_1", "start": 435595, "stop": 436011, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75414", "UniRef:UniRef90_UPI001F33AF27" ], "nt": "GTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGGATGGTTGGAAGTGGTGCGGCTGGAAGTGCTTCTAGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAAATCGCTCTTGAGATCAGCACCTGTCAGTGTTCCACCAAGCAGTACAAGTAATCAAACTTTAAGCTTATCTAACCCCGCTCCTGTGGGCCCACAAGCGGTTGTAAGCCAACCCGCGGGGGGTGCTACGGCAGCAGTGTCCGTCAATCGCACAGCGAGTGACACCGCCACCTTTAGCAAGTACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATTGTTCCGGGGTTGGAAAAATGA", "aa": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASSLQGNGSNSSGLKSLLRSAPVSVPPSSTSNQTLSLSNPAPVGPQAVVSQPAGGATAAVSVNRTASDTATFSKYLNTAQALHQMGVIVPGLEK", "aa_hexdigest": "8843f90dd8ff213ac750e6c3d8273e11", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "3-prime", "pseudogene": { "start": 435595, "stop": 436314, "observations": { "mutation": [ 436009 ], "stop": [ 436009 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F33AF27", "query_cov": 1.0, "subject_cov": 0.4726027397260274, "identity": 0.993, "score": 246.0, "evalue": 7.28e-80, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASSLQGNGSNSSGLKSLLRSAPVSVPPSSTSNQTLSLSNPAPVGPQAVVSQPAGGATAAVSVNRTASDTSTFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASRRDATSTNLPHAAGASQTGLGTGSPREPALTATSQRAVTVVAGPLRAGNSSETDALPNVITQLYHTSTAQLAYLNGQIVVMGSDRVPSLWYWVVGEDQESGKATWWAKTELNWGTDKQKQFVENQLGFKDDSNSDSKNSNLK" }, "paralog": false, "identity": 0.9707112970711297, "score": 447.0, "evalue": 2.11e-161, "description": "Internal stop codon at 436009. Nonsense mutation around 436009.", "psc": { "uniref90_id": "UniRef90_UPI001F33AF27", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_P75414", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F33AF27", "UniRef:UniRef50_P75414" ] }, "pscc": { "uniref50_id": "UniRef50_P75414", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75414" ], "product": "Putative MgpC-like protein MPN_367" } }, "id": "CNOCLNGIAL_796", "locus": "GCA000143945_00773" }, { "type": "cds", "sequence": "contig_1", "start": 436036, "stop": 436320, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTAGCCGACGGGATGCTACTTCCACTAACCTGCCCCATGCGGCAGGTGCTTCCCAAACGGGTTTGGGAACTGGTTCGCGGGGCGAACCAGCTTTAATCGCAACGTCACAGCGTGCCGTCACGGTGGTTGCTGGCCCCCTTCGTGCGGGCAATAGCAGTGAAACTGATGCCCTACCGAATGTCATCACCCAGCTCTATCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGAGTTCCGACCGGGTACCGAGTCTTTGGTATTGA", "aa": "MASRRDATSTNLPHAAGASQTGLGTGSRGEPALIATSQRAVTVVAGPLRAGNSSETDALPNVITQLYHTSTAQLAYLNGQIVVMSSDRVPSLWY", "aa_hexdigest": "2c98bc9a8d7a462fbbcd90005bfcb819", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9754.7728, "isoelectric_point": 8.291746330261233 }, "id": "CNOCLNGIAL_797", "locus": "GCA000143945_00774" }, { "type": "cds", "sequence": "contig_1", "start": 436665, "stop": 436964, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "RefSeq:WP_236253458.1", "SO:0001217", "UniParc:UPI000AAE07FB", "UniRef:UniRef100_A0AB38W8G0", "UniRef:UniRef50_A0AB38W8G0", "UniRef:UniRef90_A0AB38W8G0" ], "nt": "GTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGTTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAG", "aa": "MENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQKATAKIASLINASPLNSYGDDGDKKAIYPEVRGRQLQNIFDDWKTNKSC", "aa_hexdigest": "b113843f3eac0f70a4ce1a1d7df36ae1", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000AAE07FB", "ncbi_nrp_id": "WP_236253458.1", "uniref100_id": "UniRef100_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000AAE07FB", "RefSeq:WP_236253458.1", "UniRef:UniRef100_A0AB38W8G0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8G0", "uniref90_id": "UniRef90_A0AB38W8G0" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8G0", "product": "MgpC protein", "uniref50_id": "UniRef50_A0AB38W8G0" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_798", "locus": "GCA000143945_00775" }, { "type": "cds", "sequence": "contig_1", "start": 437526, "stop": 437900, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16526.10", "PFAM:PF01519.22", "PFAM:PF03670.18" ], "nt": "GTGAACACAGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAAATAAGATCGATAAGCTTGAAATTAAAGTTGATAAGCTAAAGAAGAAAGTCGATAAGCTGGAAGTTAAGGTTGATAAACTAGTCGAAACTGTTAATGCTCAAGGAGAAGATCTTAACAACTTCAAAGTTGAAGTGAGAGGAACACTTCAAAGCCAAGGCGAAACCCTGCAATTAATTTTGCAAACGCTACAAGACATGAGTAAGCGGCTTGATTCTGTTGAAGGACGTTTAGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTGGACAAAATCGATCCGCTAAAATAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLTKIENKIDKLEIKVDKLKKKVDKLEVKVDKLVETVNAQGEDLNNFKVEVRGTLQSQGETLQLILQTLQDMSKRLDSVEGRLDSMDGRLDSMETRLDKIDPLK", "aa_hexdigest": "63a3a07640eca0d545c9955a00cbaec6", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16526.10", "name": "CLZ", "length": 46, "aa_cov": 0.3709677419354839, "hmm_cov": 1.0, "evalue": 2.157467148876195e-07, "score": 26.727916717529297, "start": 19, "stop": 64 }, { "id": "PF01519.22", "name": "DUF16", "length": 100, "aa_cov": 0.8064516129032258, "hmm_cov": 0.89, "evalue": 1.7748499716595817e-34, "score": 113.87850189208984, "start": 2, "stop": 101 }, { "id": "PF03670.18", "name": "UPF0184", "length": 45, "aa_cov": 0.3629032258064516, "hmm_cov": 1.0, "evalue": 2.2976409229773933e-06, "score": 23.44395637512207, "start": 12, "stop": 56 } ], "seq_stats": { "molecular_weight": 14444.300699999993, "isoelectric_point": 5.350731849670409 }, "id": "CNOCLNGIAL_799", "locus": "GCA000143945_00776" }, { "type": "cds", "sequence": "contig_1", "start": 438032, "stop": 438124, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAAATTTTTAAGTACCTTTGCTAATAATACAGAAGGCTATTCATCAGATATTAATCTCTTCATTTACTTGGAATATTTAATTAAGTAA", "aa": "MNKFLSTFANNTEGYSSDINLFIYLEYLIK", "aa_hexdigest": "b0e26d257c1f12e45212b995b9cda380", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3549.9948000000013, "isoelectric_point": 4.677076148986816 }, "id": "CNOCLNGIAL_800", "locus": "GCA000143945_00777" }, { "type": "cds", "sequence": "contig_1", "start": 438188, "stop": 438457, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGATTGAATATTTCCAGATGACATACCAAAGTTTTAAGGATTTGTCAATTGAAGCTAAGTTGAGCTATACCTTTAATTGGTTTGGAGATTATTCTTCAGGTGATTTCACTGCTAAGAAAGGTGACAAGCATTATTTTGATCTTTTCCTTAAAATTAAATCTAATTCTGATCCAGGAAAACAATTTACTACAGAAAAATTTCTTACTAAAGAAGAAAAAATTAAAGTTAATGGAAAAGAAATTACAAGAAATTTAGAGTGA", "aa": "MERKIEYFQMTYQSFKDLSIEAKLSYTFNWFGDYSSGDFTAKKGDKHYFDLFLKIKSNSDPGKQFTTEKFLTKEEKIKVNGKEITRNLE", "aa_hexdigest": "cfdf950205690ee16424a3112fa284e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10600.865199999991, "isoelectric_point": 8.753146553039553 }, "id": "CNOCLNGIAL_801", "locus": "GCA000143945_00778" }, { "type": "cds", "sequence": "contig_1", "start": 438545, "stop": 438793, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGCGCAAAAAGAAGTTTTTGTCAAAGGTTTCCTTTGGTTCTTTATTTTTGTTATGTGGAACAGTTTTAAGCGCCTGTACTGGTATCCAAGCAGATCTTAGAAATCTTATTAAAGAAGCCACCGGTAAAGACATTGATGTGTATAAAGCAATTAAAACTACAGAAGGCAAAAAGAACATGATAACTTCTCTAAAGAAGTCTTACAAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGA", "aa": "MRKKKFLSKVSFGSLFLLCGTVLSACTGIQADLRNLIKEATGKDIDVYKAIKTTEGKKNMITSLKKSYKVNPKDTTKLLLDA", "aa_hexdigest": "9a74f050e0e7a42e1d591c8b79760ceb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 0.8290000000000001, "score": 134.0, "evalue": 1.63e-39, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "CNOCLNGIAL_802", "locus": "GCA000143945_00779" }, { "type": "cds", "sequence": "contig_1", "start": 439269, "stop": 439388, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0AAN4XEA6" ], "nt": "ATGGTAGCTTACTTTGATCCAAAGGAAGACGGTACTTGTGAATTCATTATGAAAATTAAAACTGTAAATGAAGTTGCTGACACTGAGATAACAAATAAGGAAAAGCCTTTAGGCACTTGA", "aa": "MVAYFDPKEDGTCEFIMKIKTVNEVADTEITNKEKPLGT", "aa_hexdigest": "230972b44fd8b8153b632bd9c81fe607", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 439167, "stop": 439397, "observations": { "mutation": [ 439248, 439386 ], "stop": [ 439248, 439386 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN4XEA6", "query_cov": 1.0, "subject_cov": 0.5131578947368421, "identity": 1.0, "score": 83.2, "evalue": 3.49e-20, "gene_start": 1, "gene_end": 39, "reference_start": 35, "reference_end": 73, "reference_sequence": "EKRKEHLNMKKVKALRNLFLEKASEKLEYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVADTEITNKEKPLGTWKS" }, "paralog": false, "identity": 0.9605263157894737, "score": 145.0, "evalue": 3.2e-47, "description": "Internal stop codon at 439248, 439386. Nonsense mutation around 439248, 439386.", "psc": { "uniref90_id": "UniRef90_A0AAN4XEA6", "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN4XEA6", "UniRef:UniRef50_A0A0H3DJX7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4407.0016000000005, "isoelectric_point": 4.554986000061036 }, "id": "CNOCLNGIAL_803", "locus": "GCA000143945_00780" }, { "type": "cds", "sequence": "contig_1", "start": 439440, "stop": 439772, "strand": "+", "frame": 3, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQB0", "UniRef:UniRef90_A0AB33HQB0" ], "nt": "GTGTTAAAACTTGCTGTTGGTATTTTTATTTCCCCCACGCTCACCAGGTTTAGTACCGGGTTCAACCTCGCGGTGTCGGTGCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGCACAGGTATGGGAATAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTGGGTGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGA", "aa": "MLKLAVGIFISPTLTRFSTGFNLAVSVLDQVLDYVPWIGNGHRYGNNHRGVDDITAPKTGAGSSSGTSTNTSGSRSFLPTFSNVGVGLKANVQGTLGGSQTTTTGKDIPK", "aa_hexdigest": "ea5f52ba7b5cd63fcd3ab7193cd9810f", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 439440, "stop": 440075, "observations": { "mutation": [ 439770, 439803, 439818 ], "stop": [ 439770, 439803, 439818 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HQB0", "query_cov": 1.0, "subject_cov": 0.41509433962264153, "identity": 0.991, "score": 211.0, "evalue": 3.18e-67, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MLKLAVGIFISPTLTRFSTGFNLAGSVLDQVLDYVPWIGNGHRYGNNHRGVDDITAPKTGAGSSSGTSTNTSGSRSFLPTFSNVGVGLKANVQGTLGGSQTTTTGKDIPKWPTLDPAKLQLWTGAGWRNDKASNKQSDENHTTFKSATGSGQQGGSTTGGSAGNPDSLKQDKISKSGQNLTTQDGAPQSNSTTESASNYDHLPPPTSPPHPIDRTRCHSPTRTTRSAPSSSSAACWAASRCWWIEVGQMIPTNSKPPTKNGLTPT" }, "paralog": false, "identity": 0.9622641509433962, "score": 404.0, "evalue": 2.12e-145, "description": "Internal stop codon at 439770, 439803, 439818. Nonsense mutation around 439770, 439803, 439818.", "psc": { "uniref90_id": "UniRef90_A0AB33HQB0", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HQB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HQB0", "UniRef:UniRef50_A0AB33HQB0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HQB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQB0" ], "product": "Adhesin P1 homolog" } }, "id": "CNOCLNGIAL_804", "locus": "GCA000143945_00781" }, { "type": "cds", "sequence": "contig_1", "start": 440156, "stop": 440437, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATCGAAGTGGGTCAGATGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCTTACACCGACTTAAAGTCGGACCAAACCAAGCTCAACCTCCCCGCTTATGGTGAAGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDDSNKFQATDQKWSYTDLKSDQTKLNLPAYGEVNGLLNPALVETYFGTTRAGGSGSNTTSSPGIGFKIPEQNNDSKAVLITPGLA", "aa_hexdigest": "2649d38c15df25edcb4701e922358f22", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9868.75109999999, "isoelectric_point": 5.125819969177246 }, "id": "CNOCLNGIAL_805", "locus": "GCA000143945_00782" }, { "type": "cds", "sequence": "contig_1", "start": 440932, "stop": 441249, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_019830409.1", "SO:0001217", "UniParc:UPI000364214F", "UniRef:UniRef100_UPI000364214F", "UniRef:UniRef50_UPI000364214F", "UniRef:UniRef90_UPI000364214F", "PFAM:PF03257.18" ], "nt": "TTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAAGCCAAACCCCAATCCCTCAAAAAAACCACACCGGTGTTTGGGACGAATAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGACAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAATTACCCAGTGGGGGTGGGGGGAATAGTAGTGAGGATATTAAAAGTGTGCAAGACACTCCTATTTATGATCTATATTCATTAGTATATTCTTTTTAA", "aa": "MDPNQVRTKLRQSFGTDHSTQAKPQSLKKTTPVFGTNSGNIGSVLSGGGAGGADSTNSVDLSPVERVSGWLVGQLPSGGGGNSSEDIKSVQDTPIYDLYSLVYSF", "aa_hexdigest": "f7bdc227d18dc70836735476be5a44cb", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000364214F", "ncbi_nrp_id": "WP_019830409.1", "uniref100_id": "UniRef100_UPI000364214F", "db_xrefs": [ "SO:0001217", "UniParc:UPI000364214F", "RefSeq:WP_019830409.1", "UniRef:UniRef100_UPI000364214F" ] }, "ips": { "uniref100_id": "UniRef100_UPI000364214F", "uniref90_id": "UniRef90_UPI000364214F" }, "psc": { "uniref90_id": "UniRef90_UPI000364214F", "uniref50_id": "UniRef50_UPI000364214F" }, "pscc": { "uniref50_id": "UniRef50_UPI000364214F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000364214F" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 71, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 1.8742615420716318e-26, "score": 88.32891082763672, "start": 1, "stop": 70 } ], "seq_stats": { "molecular_weight": 10906.823099999998, "isoelectric_point": 5.600084495544433 }, "id": "CNOCLNGIAL_806", "locus": "GCA000143945_00783" }, { "type": "cds", "sequence": "contig_1", "start": 441492, "stop": 441653, "strand": "+", "frame": 3, "gene": null, "product": "Cytadhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6U4", "UniRef:UniRef90_A0AB38W6U4" ], "nt": "GTGCGCGACAACACCGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCACCGCCACCACCTTTGCGTTACGCCGATACGACCTCGCCGGGCGTTGTACGACCTCGACTTTTCGAAGTTAA", "aa": "MRDNTAKGITAGSGSQQTTYDPTRTEAALTTATTFALRRYDLAGRCTTSTFRS", "aa_hexdigest": "862b350769932b5f7791aa830593b780", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 441309, "stop": 441653, "observations": { "mutation": [ 441423 ], "stop": [ 441423 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W6U4", "query_cov": 1.0, "subject_cov": 0.4649122807017544, "identity": 0.981, "score": 100.0, "evalue": 2.56e-26, "gene_start": 1, "gene_end": 53, "reference_start": 62, "reference_end": 114, "reference_sequence": "MGHFTSTTTTLKRQQFSYTRPDEVALRHTNAINPRLTPWTYRNTSFSSLPLTGENPGAWALVRDNTAKGITAGSGSQQTTYDPTRTEAALTTATTFALRRYDLAGRCTTSTFRS" }, "paralog": false, "identity": 0.9824561403508771, "score": 228.0, "evalue": 4.27e-79, "description": "Internal stop codon at 441423. Nonsense mutation around 441423.", "psc": { "uniref90_id": "UniRef90_A0AB38W6U4", "product": "Cytadhesin P1", "uniref50_id": "UniRef50_A0AB38W6U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W6U4", "UniRef:UniRef50_A0AB38W6U4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6U4" ], "product": "Cytadhesin P1" } }, "id": "CNOCLNGIAL_807", "locus": "GCA000143945_00784" }, { "type": "cds", "sequence": "contig_1", "start": 441776, "stop": 442267, "strand": "+", "frame": 2, "gene": null, "product": "Adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321", "UniRef:UniRef90_A0AB38W8Y4" ], "nt": "GTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTP", "aa_hexdigest": "4bcf7107068d6cb7dd24f3a658688ab8", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "both", "pseudogene": { "start": 441626, "stop": 442423, "observations": { "mutation": [ 441743, 442265 ], "stop": [ 441743, 442265 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8Y4", "query_cov": 1.0, "subject_cov": 0.6150943396226415, "identity": 0.9940000000000001, "score": 254.0, "evalue": 2.71e-83, "gene_start": 1, "gene_end": 163, "reference_start": 51, "reference_end": 213, "reference_sequence": "MYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKNKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTPWTPWLTTEQIHNDPAKFAASILILYDAPYARNRTVIDRVDHLDPKVMVDNYC" }, "paralog": false, "identity": 0.9886792452830189, "score": 475.0, "evalue": 1.61e-172, "description": "Internal stop codon at 441743, 442265. Nonsense mutation around 441743, 442265.", "psc": { "uniref90_id": "UniRef90_A0AB38W8Y4", "product": "Adhesin P1", "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8Y4", "UniRef:UniRef50_P75321" ] }, "pscc": { "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321" ], "product": "Uncharacterized protein MPN_462" } }, "id": "CNOCLNGIAL_808", "locus": "GCA000143945_00785" }, { "type": "cds", "sequence": "contig_1", "start": 442485, "stop": 442928, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02917.20", "PFAM:PF22596.2" ], "nt": "ATGCCAAATCCTGTTAGATTTGTTTACCGTGTTGATTTGAGAAGCCCTGAAGAAATTTTTGAACATGGCTTTTCAACTTTAGGTGATGTGAGAAATTTCTTTGAACACATTCTCTCCACTAATTTTGGTAGAAGCTATTTTATTTCCACTTCAGAAACACCCACAGCAGCTATTCGCTTCTTTGGTAGCTGGTTACGGGAATATGTACCAGAGCACCCCAGAAGGGCTTACTTATATGAAATTCGTGCCGACCAACACTTTTACAATGCCCGCGCCACTGGGGAGAACTTGTTAGATTTAATGCGTCAAAGACAAGTAGTATTTGACTCTGGTGATCGAGAAATGGCACAAATGGGAATTAGAGCTTTACGCACTTCCTTTGCGTATCAACGTGAATGGTTTACCGATGGTCCAATTGCAGCAGCTAATGTCCGTAGTGCTTGA", "aa": "MPNPVRFVYRVDLRSPEEIFEHGFSTLGDVRNFFEHILSTNFGRSYFISTSETPTAAIRFFGSWLREYVPEHPRRAYLYEIRADQHFYNARATGENLLDLMRQRQVVFDSGDREMAQMGIRALRTSFAYQREWFTDGPIAAANVRSA", "aa_hexdigest": "991ddd04da404e2e6271f5eeddd807ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02917.20", "name": "Pertussis_S1", "length": 146, "aa_cov": 0.9727891156462585, "hmm_cov": 1.0, "evalue": 2.5935859468743627e-83, "score": 274.9464111328125, "start": 3, "stop": 145 }, { "id": "PF22596.2", "name": "Scabin-like", "length": 114, "aa_cov": 0.7278911564625851, "hmm_cov": 0.9298245614035088, "evalue": 4.5483394317005427e-08, "score": 28.53148651123047, "start": 8, "stop": 114 } ], "seq_stats": { "molecular_weight": 17225.12139999999, "isoelectric_point": 6.919317436218263 }, "id": "CNOCLNGIAL_809", "locus": "GCA000143945_00786" }, { "type": "cds", "sequence": "contig_1", "start": 443343, "stop": 443576, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22509.2" ], "nt": "ATGTTACAAGCAGATCCGCAAAATAACAATGTCTTTTTGGTCGAAGTAAACCCCAAACAAAAGTCCAGCTTTCCCCAAACCATCTTCTTTTGGGATGTTTATCAACGAATTTGTCTCAAGGATTTAACTGGTGCACAAATCAGTCTTTCGCTTACTGCCTTTACTACTCAGTATGCTGGTCAGCTCAAAGTGCACCTTAGTGTTAGCGCGGTTAATGCCGTGAACCAAAAGTGA", "aa": "MLQADPQNNNVFLVEVNPKQKSSFPQTIFFWDVYQRICLKDLTGAQISLSLTAFTTQYAGQLKVHLSVSAVNAVNQK", "aa_hexdigest": "6150e794e3b170ad6ba22a8f853ffe68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22509.2", "name": "CARDS-D2_trefoil", "length": 84, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 5.960646258546392e-38, "score": 125.75577545166016, "start": 1, "stop": 77 } ], "seq_stats": { "molecular_weight": 8644.801099999999, "isoelectric_point": 8.931723976135256 }, "id": "CNOCLNGIAL_810", "locus": "GCA000143945_00787" }, { "type": "cds", "sequence": "contig_1", "start": 443880, "stop": 444008, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22346.2" ], "nt": "ATGAAAACACCAGATGGACAGATATTCTATGATCTAAAAACTTCGAAAATCTTCTTTGTCCAGGACAACCAAAACGTGTTCTTTCTCCATAATAAACTCAACAAACAAACTGGTTACAGCTGGGATTGA", "aa": "MKTPDGQIFYDLKTSKIFFVQDNQNVFFLHNKLNKQTGYSWD", "aa_hexdigest": "04c19e688bad2cd46fd20e5ed0ad5150", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22346.2", "name": "CARDS_D3", "length": 42, "aa_cov": 1.0, "hmm_cov": 0.9761904761904762, "evalue": 1.0241559187984014e-17, "score": 59.96404266357422, "start": 1, "stop": 42 } ], "seq_stats": { "molecular_weight": 5057.648300000002, "isoelectric_point": 8.085834312438966 }, "id": "CNOCLNGIAL_811", "locus": "GCA000143945_00788" }, { "type": "cds", "sequence": "contig_1", "start": 444030, "stop": 444260, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22346.2" ], "nt": "ATGAATGAGGACAAAGACGAAAACTTTAAATGGTACTTTTCGCGTGATGACCTTACCATTCCTTCCGTTGAAGGGCTTAACTTCCGCCACATTCGCTGTTACGCTGACAACCAGCAGTTAAAGGTGATCATAAGCGGTTCACGTTGGGGCGGTTGGTACTCCACTTACGATAAAGTTGAAAGTAATGTCGAAGATAAGATTTTGGTCAAAGATGGTTTTGATCGCTTTTAG", "aa": "MNEDKDENFKWYFSRDDLTIPSVEGLNFRHIRCYADNQQLKVIISGSRWGGWYSTYDKVESNVEDKILVKDGFDRF", "aa_hexdigest": "16378f58107b3c4b6ceee164ce8966fb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22346.2", "name": "CARDS_D3", "length": 68, "aa_cov": 0.8947368421052632, "hmm_cov": 1.0, "evalue": 4.7471478162101495e-34, "score": 112.91462707519531, "start": 1, "stop": 68 } ], "seq_stats": { "molecular_weight": 9086.969899999996, "isoelectric_point": 4.984916114807128 }, "id": "CNOCLNGIAL_812", "locus": "GCA000143945_00789" }, { "type": "cds", "sequence": "contig_1", "start": 444277, "stop": 445134, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BC084C", "UniRef:UniRef90_UPI0035BC084C" ], "nt": "TTGAAGTCCAACGAGAGTTCTAAACGTGGTGTTTTAAGTCAAGCTGCTAGTGGTGCGGTGGTTGGTTATGTAGAGAGACTAACTAACAAGAAGGTTTTCTTAAAGGACGAAATTAAGTTTGATGTTGAAAAGGATGGCATCAAGCTAGACTTAAAAGCCGACTTCTCGCTTGAACAAAATGGCTCTTTAGGTTTGAATGACCCAAATGATGTCAACAAATTGGCACAGGAAGCACAATCTTACACAGTTATGGTAAGTGATGGCGGTGCCCAAACTGACAATAATGCTGAAGGTGGTAATCTAAGAATTGCCTTAACTAAGAATGCATTCAACCCAAAACAAAGCACTACGGTTGATATTCCTTACAAAATAGAAAATAGAAGTGTGGGAAATAATAAGGAGCAGAAAACACTTGTGTTTGATTTCTCTGGACTCAATCCGTATGAGTACAACATGATTGTAGGGGCATTGTTTACCGACAGCTCATTTATTAATGATGCTTATGCACCAATTCAAAGTACCTTCCAAAGACAATTGAAGGAGTTCTTACAAGTTAAGTACGAAAACCAAGTTGGTGCTAATGGTTCATTTGACCTCTTCAAACCACGCTCATTGTCTAGCCAACAACTAGTTCAAGGTGAAAGATCACTAGATGGCTTTACCGTTGAATTGAATGCAAATGGTGGCAGTTTCAACTTCTTAACCCACGTAGATCCGTTGGTAGCTGGTTTAACTGTGGCTGCTATAGCTAGTGTAGTAGCTGCTGGTGCTGTAACTTATTTAGTAGTACGTCGTTACCGCAAGCGCAACGAGTTTGTCGACAAGATCTTTGCTTCTAACATTAGAGCAAAACAGTGA", "aa": "MKSNESSKRGVLSQAASGAVVGYVERLTNKKVFLKDEIKFDVEKDGIKLDLKADFSLEQNGSLGLNDPNDVNKLAQEAQSYTVMVSDGGAQTDNNAEGGNLRIALTKNAFNPKQSTTVDIPYKIENRSVGNNKEQKTLVFDFSGLNPYEYNMIVGALFTDSSFINDAYAPIQSTFQRQLKEFLQVKYENQVGANGSFDLFKPRSLSSQQLVQGERSLDGFTVELNANGGSFNFLTHVDPLVAGLTVAAIASVVAAGAVTYLVVRRYRKRNEFVDKIFASNIRAKQ", "aa_hexdigest": "802ee10bafb3880560e4674731494bb1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0035BC084C", "query_cov": 1.0, "subject_cov": 0.9930313588850174, "identity": 0.993, "score": 538.0, "evalue": 6.92e-193, "valid": true, "uniref50_id": "UniRef50_UPI0035BC084C" }, "pscc": { "uniref50_id": "UniRef50_UPI0035BC084C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BC084C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31318.789599999993, "isoelectric_point": 7.763717460632326 }, "id": "CNOCLNGIAL_813", "locus": "GCA000143945_00790" }, { "type": "cds", "sequence": "contig_1", "start": 445213, "stop": 445506, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACGGAAATGAAAGCAATGGTCACTTCTATGTGGTAGTAGATGTAACTAACGCTAACAACCTTGGTAACCAACGTCGTATTGCTAACCCTAGAAACTATTTCTATTACCTAGAAGGTTTAGACAAGAACGCGCAAAGTACTTACATTATCCGTTTTGAAACAAAGGATAAGTTCTACTCACTCGATGCACTTAGGTTCGACAACAACGGTATATACGTCGATAATGCCTCAAGGGACGCTGTAATTCAAGCAAGACAAAAATCTAAACTGTATTTCAACACTAGTGATTGA", "aa": "MNGNESNGHFYVVVDVTNANNLGNQRRIANPRNYFYYLEGLDKNAQSTYIIRFETKDKFYSLDALRFDNNGIYVDNASRDAVIQARQKSKLYFNTSD", "aa_hexdigest": "81e8e0c4f9a1f5f5b261add98bfc2149", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11273.250799999985, "isoelectric_point": 7.946776008605957 }, "id": "CNOCLNGIAL_814", "locus": "GCA000143945_00791" }, { "type": "cds", "sequence": "contig_1", "start": 445926, "stop": 446066, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCATTTACGCTAACGTTACCCTAGGACAAAAAGTAACTAAGGAAGTTAAAGTGTGGGATGGACAAAACTTCCAGTTCTTAGCAATTGAAAACAATGAAGACCAAGCTCAATGCTTTAGCAAACGAAAACAAAGCTGA", "aa": "MAIYANVTLGQKVTKEVKVWDGQNFQFLAIENNEDQAQCFSKRKQS", "aa_hexdigest": "4652c0566a95aeb7e765497ed42c4a6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5306.939300000001, "isoelectric_point": 7.884370613098144 }, "id": "CNOCLNGIAL_815", "locus": "GCA000143945_00792" }, { "type": "cds", "sequence": "contig_1", "start": 446169, "stop": 446603, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_374", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75407", "UniRef:UniRef90_P75407" ], "nt": "GTGAAAGAGGTGATTGACACTAACACCACTGGAACTAACTTTAAGTTTGAAAACAGAAATTACAAACTACAAAACCAAAGCTTTAGTTTTGTCCGTTACAAAAACTTTAACTTAGATAAGCTGTTGATCCCTAAAACAAACGCTAATCTAAATTCAACTAGAAACGGTGTTTTAACTCAAGCACCTACCTTAGATTTTGTTTTAAATCCAGTTTTTGTTAATGCAATCACTAACGTTTACAACCTTTCTGAACAAGTAAAGGATTTAGAACAACGTTTAGAGAGCAAAAGTAACGAAAGTGAAAAAGCTGGCATTAACAGCGTTTTAACGCAAATTAAAAACAAGCAAGTTGATTACCTAAAGGTAAAAGAATACATTAGTAGCCTAGATAAAGATACCAATAAAGTAAGTACTCCAATTAACTCAACCGGTTGA", "aa": "MKEVIDTNTTGTNFKFENRNYKLQNQSFSFVRYKNFNLDKLLIPKTNANLNSTRNGVLTQAPTLDFVLNPVFVNAITNVYNLSEQVKDLEQRLESKSNESEKAGINSVLTQIKNKQVDYLKVKEYISSLDKDTNKVSTPINSTG", "aa_hexdigest": "8cec4020a07a8bbd04c976782f08d0e6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 445914, "stop": 446603, "observations": { "mutation": [ 445928, 446105, 446171 ], "stop": [ 445928, 446105, 446171 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75407", "query_cov": 1.0, "subject_cov": 0.62882096069869, "identity": 1.0, "score": 272.0, "evalue": 3.86e-91, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MKEVIDTNTTGTNFKFENRNYKLQNQSFSFVRYKNFNLDKLLIPKTNANLNSTRNGVLTQAPTLDFVLNPVFVNAITNVYNLSEQVKDLEQRLESKSNESEKAGINSVLTQIKNKQVDYLKVKEYISSLDKDTNKVSTPINSTGWVKWYQEANKELGLNLNQEPKDWDQFLKLVASYFSMAIYANVTLGQKVTKEVKVWDGQNFQFLAIENNEDQAQCFSKRKQSWNYC" }, "paralog": false, "identity": 0.982532751091703, "score": 446.0, "evalue": 7.8e-162, "description": "Internal stop codon at 445928, 446105, 446171. Nonsense mutation around 445928, 446105, 446171.", "psc": { "uniref90_id": "UniRef90_P75407", "product": "Uncharacterized protein MPN_374", "uniref50_id": "UniRef50_P75407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75407", "UniRef:UniRef50_P75407" ] }, "pscc": { "uniref50_id": "UniRef50_P75407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75407" ], "product": "Uncharacterized protein MPN_374" } }, "id": "CNOCLNGIAL_816", "locus": "GCA000143945_00793" }, { "type": "cds", "sequence": "contig_1", "start": 446949, "stop": 447140, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_375", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75406", "UniRef:UniRef90_P75406" ], "nt": "GTGCTCATTGAAAGGGGTGAACTTCACCGTCAAGCAGATTTAAAAAAATCTGACCCCGCAACAAACGAAACTTCCAAAACCGTTTGGGGTAGCATTAAAGAAAAGCTGTTACAAAACGTCAACAACCTCCACTCAGAGAAAGGAGTATTCTTATGGTTCCGTCAATCTGGTTTTACAACTACTAGAAACTAG", "aa": "MLIERGELHRQADLKKSDPATNETSKTVWGSIKEKLLQNVNNLHSEKGVFLWFRQSGFTTTRN", "aa_hexdigest": "fed5dd3bc384590074c0c74989a3d5c8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 446949, "stop": 447338, "observations": { "mutation": [ 447182 ], "stop": [ 447182 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75406", "query_cov": 1.0, "subject_cov": 0.4883720930232558, "identity": 0.9840000000000001, "score": 129.0, "evalue": 2.32e-37, "gene_start": 1, "gene_end": 63, "reference_start": 67, "reference_end": 129, "reference_sequence": "MSFGLVGTVNNNGWKSPFRHETKYRAGYDKFKYYKTHYRGAKKAGTNDDRWRWTAWFDLDFAHQKIVLIERGELHRQADLKKSDPATNETSKTVWGSIKEKLLQNVNNLHSEKGVFLWFRQSGFTTTRN" }, "paralog": false, "identity": 0.9922480620155039, "score": 273.0, "evalue": 2.34e-96, "description": "Internal stop codon at 447182. Nonsense mutation around 447182.", "psc": { "uniref90_id": "UniRef90_P75406", "product": "Uncharacterized protein MPN_375", "uniref50_id": "UniRef50_P75406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75406", "UniRef:UniRef50_P75406" ] }, "pscc": { "uniref50_id": "UniRef50_P75406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75406" ], "product": "Uncharacterized protein MPN_375" } }, "id": "CNOCLNGIAL_817", "locus": "GCA000143945_00794" }, { "type": "cds", "sequence": "contig_1", "start": 447856, "stop": 449448, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_376", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75405", "UniRef:UniRef90_P75405" ], "nt": "ATGAAGGTTTGGGATGGTGCTAAGTTTGAGTTCATCCCGATTGAAAACACTGAAAAACAATCAGAACAACTAGCAAATGAAAACCGCGCTGAGATTGCTGTAAGCGCCATTGGTTTTCAAGATGAAGGCGGTTTACGTGATGCTAGTTTTATCAATAAGGTAGCTCTAACCCCTAAGAGTAGTAAAACTAAAATTGCTAACGGTGATGCTAGCAAAATAGAAAAAGCAGCCAACGAAATTTCTTATAAGTACCATTACCGACAAAACTTTAAACAAGCTAGCTGGGACAAACAAAACTCACAAACCAAGAGTATTGTAGTACAAAGCACTGATTTGAACGATGAGCGCGAACGCTTCCAAAAGGACATTAACAACTATTTAAAGGTACAAGGCATCTCTGAAACTGAAATTAAAGTAAATGCTGTTCATAAAGTTGATGCGATGTTAAACGCCCGCAAAAGTGATGATCCGAAGCTCGCTAGCGTTCAAAGTACGGCAAACAAGTATGGTTTAAACCTACGATCTAACCCATACACTGGTCAGTTTTATGTAGTAGTGGATGTAACTAATGCCAATGACTTGGGTAACCAACGCCGGGCAAATAACGCTAAGAGTTACTTTTACTATATTGAAGGGTTGGACAAAGGAGCACAAAGTTCGTACTTAGTGCGCTTTGAAAACAAACAAAAGCTGTATTCACTCGAATCACTAGCGGTTGATAGTCGTGGCCTTTATGTGAAAAATGTGTCCAAGGATGCAATTATCCAAGCCAAGCAAAACCAAAACCTTTATCTCGATACTCATAACTGGAACGCTGCTTTGAAGGCTAACCTCACCAACGCTGAACTTACTTTGCCTACAGCAAGTGCTGACAATTCAGCTAAACTATCTACACCTAACGCTGAGAATGATGAGGGTTTTTTGAGTGAAAATGTCAGTGGTTCGATCTTAGGCTATGTTGAGCGAATGACTGGTAAAAAGCTCTTTTTAAAGGAAAGGGTGAGCTTTAATAAGGAAGACAAAAATAATTTAAAGCTACGTTTAACATCGAACTTTACCTTAGACAAGAAGGGTAATTTAGAGGTTAAAGACCCAAGTGTAATTAACCAAATTGTAGAAGAAGCTAAGGGTTACAATGTCTTGGTTTCAGAAGAAAAAGGTGATGATCCAGAATCAGACAAAAACATCTTCAAGATTACTTTAACCACCAACCCTGAACAAAGTACCGTTATTAAACTGCCTTACTGGATTGTTACCAAGAAATCCAAAACTAACAAGGATGGTACGGTTAGAGAGCAGAAAAACTTGGTGTTTGACTTCTCTAACCTAAACAACTTCGAATACAACACAGTTGTTAGTCTGTTGTTTACCGACAGCTCCTTTATTAAGAATGCCTACGCTCCATTGCAAACAGAATTTAGAAAGCAGCTCAAAACCGTTTTAGAACACAAATACCAAGCACCAATTAAGACTGGTCAACTACCTTTATTGACCAAGGTACAATTGGCAAATAACCAAAAACAAATTGATAACTTCACCTTTGATCTACACAAAAACATTTTCAATAAAGAAGACATCAATAAGATTAACTGA", "aa": "MKVWDGAKFEFIPIENTEKQSEQLANENRAEIAVSAIGFQDEGGLRDASFINKVALTPKSSKTKIANGDASKIEKAANEISYKYHYRQNFKQASWDKQNSQTKSIVVQSTDLNDERERFQKDINNYLKVQGISETEIKVNAVHKVDAMLNARKSDDPKLASVQSTANKYGLNLRSNPYTGQFYVVVDVTNANDLGNQRRANNAKSYFYYIEGLDKGAQSSYLVRFENKQKLYSLESLAVDSRGLYVKNVSKDAIIQAKQNQNLYLDTHNWNAALKANLTNAELTLPTASADNSAKLSTPNAENDEGFLSENVSGSILGYVERMTGKKLFLKERVSFNKEDKNNLKLRLTSNFTLDKKGNLEVKDPSVINQIVEEAKGYNVLVSEEKGDDPESDKNIFKITLTTNPEQSTVIKLPYWIVTKKSKTNKDGTVREQKNLVFDFSNLNNFEYNTVVSLLFTDSSFIKNAYAPLQTEFRKQLKTVLEHKYQAPIKTGQLPLLTKVQLANNQKQIDNFTFDLHKNIFNKEDINKIN", "aa_hexdigest": "870cdcc48cbd6877478a28c061d72697", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 447718, "stop": 449748, "observations": { "mutation": [ 447753, 447777, 447858, 449535, 449643 ], "stop": [ 447753, 447777, 447858, 449535, 449643 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75405", "query_cov": 1.0, "subject_cov": 0.4649122807017544, "identity": 1.0, "score": 1013.0, "evalue": 0.0, "gene_start": 1, "gene_end": 530, "reference_start": 565, "reference_end": 1094, "reference_sequence": "MKRLQYRFLLFGFALGSFGWFVASSAFTSVVKSNNETLSSDGGVHMRYLKGKGDVLSILKSKSPAITQRFDLKWNNGKKTEVYLNNVNNYISESLYAQEALKQIERTAQLSIQNGELPDVKVYTNSGASFGLVGWHTDNGRTWAFRHENKYDKNVHFSWNQTYYKYSNRERTASNPYYWKWVAWFDLGYANQRIGLVENDEYHIDKRVPEPTPRKWDKNKPLWGDIRSKILYSAERLDPDKGIFIWFNQTGFNTKGTKGWANSGFFTDFWDTNNNPNAFTTNITSEGGNSNWHSPDWGSHTTDTRFFLKLEPYSKLFYKENGQERSITVSDYIRKAKSTKTNYQWVNKNQIKTLVRKTRSIDLGLGSAVRQTYTTKSDIASNQQLKYKLKDSTFSIDTYNNFKLDKLLVPKTNEDATAIKNGVFVKQPTLSFDFNPVLTNAIVNIHNLFAQTLDLKEHLKSDQPYNESDKAAINKVIEQIKNKEVDYIQVADFIGKLKNWSQNPGSIESKGENTAQWYADAKREFGLNLNDDVNTWTQLSSLIASYFSKDIFANVKLNGAKERRMKVWDGAKFEFIPIENTEKQSEQLANENRAEIAVSAIGFQDEGGLRDASFINKVALTPKSSKTKIANGDASKIEKAANEISYKYHYRQNFKQASWDKQNSQTKSIVVQSTDLNDERERFQKDINNYLKVQGISETEIKVNAVHKVDAMLNARKSDDPKLASVQSTANKYGLNLRSNPYTGQFYVVVDVTNANDLGNQRRANNAKSYFYYIEGLDKGAQSSYLVRFENKQKLYSLESLAVDSRGLYVKNVSKDAIIQAKQNQNLYLDTHNWNAALKANLTNAELTLPTASADNSAKLSTPNAENDEGFLSENVSGSILGYVERMTGKKLFLKERVSFNKEDKNNLKLRLTSNFTLDKKGNLEVKDPSVINQIVEEAKGYNVLVSEEKGDDPESDKNIFKITLTTNPEQSTVIKLPYWIVTKKSKTNKDGTVREQKNLVFDFSNLNNFEYNTVVSLLFTDSSFIKNAYAPLQTEFRKQLKTVLEHKYQAPIKTGQLPLLTKVQLANNQKQIDNFTFDLHKNIFNKEDINKINWPLIAITFTGSAALLSTIIASGVVLHRWRKSRKHFWEQMLKARKVK" }, "paralog": false, "identity": 0.9911242603550295, "score": 1306.0, "evalue": 0.0, "description": "Internal stop codon at 447753, 447777, 447858, 449535, 449643. Nonsense mutation around 447753, 447777, 447858, 449535, 449643.", "psc": { "uniref90_id": "UniRef90_P75405", "product": "Uncharacterized protein MPN_376", "uniref50_id": "UniRef50_P75405", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75405", "UniRef:UniRef50_P75405", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75405" ], "product": "Uncharacterized protein MPN_376" } }, "id": "CNOCLNGIAL_818", "locus": "GCA000143945_00795" }, { "type": "cds", "sequence": "contig_1", "start": 449641, "stop": 449916, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAAAAACCAACGAGGATGCTACGGCAATTAAGAATGGTGTTTTTGTCAAACAACCAACACTGTCCTTTGACTTTAACCCCGTTTTGACAAACGCCATTGTCAACATTCACAATCTGTTTGCGCAAACACTTGATTTAAAGGAGCATTTAAAGTCTGATCAACCTTACAATGAAAGTGACAAAGCAGCAATCAATAAGGTTATAGAACAAATCAAAAATAAGGAAGTTGACTACATTCAAGTAGCTGATTTTATTGGCAAGCTGAAAAATTGA", "aa": "MPKTNEDATAIKNGVFVKQPTLSFDFNPVLTNAIVNIHNLFAQTLDLKEHLKSDQPYNESDKAAINKVIEQIKNKEVDYIQVADFIGKLKN", "aa_hexdigest": "c56e106ad43a8b3a1c109fc22c6d28d9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10286.595499999992, "isoelectric_point": 6.056785774230956 }, "id": "CNOCLNGIAL_819", "locus": "GCA000143945_00796" }, { "type": "cds", "sequence": "contig_1", "start": 450250, "stop": 450597, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTGGTTTGATTTGGGTTATGCCAACCAACGCATAGGTTTGGTGGAAAATGATGAATATCATATTGACAAGCGTGTTCCAGAACCAACTCCCAGAAAATGGGATAAAAACAAACCTTTATGGGGTGATATTCGCTCGAAGATTCTCTACAGTGCTGAGCGGTTAGATCCAGATAAGGGAATCTTCATCTGGTTCAACCAAACTGGTTTTAACACCAAGGGTACTAAGGGCTGGGCTAACAGTGGCTTTTTTACCGATTTTTGGGATACCAACAATAACCCCAATGCCTTTACCACCAACATCACTTCTGAAGGTGGTAACTCCAATTGGCACAGTCCTGATTGA", "aa": "MAWFDLGYANQRIGLVENDEYHIDKRVPEPTPRKWDKNKPLWGDIRSKILYSAERLDPDKGIFIWFNQTGFNTKGTKGWANSGFFTDFWDTNNNPNAFTTNITSEGGNSNWHSPD", "aa_hexdigest": "1557b8f18486d770c223a0cfc85380d7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13330.473299999983, "isoelectric_point": 5.623558998107909 }, "id": "CNOCLNGIAL_820", "locus": "GCA000143945_00797" }, { "type": "tRNA", "sequence": "contig_1", "start": 451404, "stop": 451480, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 74.9, "nt": "GCGCCCATAGCTCAATTGGATAGAGTGTCTGGCTTCGGACCAGAAGGTTATGGGTTCAAGTCCTATTGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 451444, 451446 ], "id": "CNOCLNGIAL_31", "locus": "GCA000143945_00798" }, { "type": "cds", "sequence": "contig_1", "start": 451566, "stop": 451790, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_377", "db_xrefs": [ "RefSeq:WP_014325500.1", "SO:0001217", "UniParc:UPI0001DD63C9", "UniRef:UniRef100_A0AAX0SQJ9", "UniRef:UniRef50_Q9EXD3", "UniRef:UniRef90_Q9EXD3" ], "nt": "ATGTCAAAGGATAAAAAAAATAAGGTTGAACAGCTTGAACCAGTAGACCTTTTTGAGCGGACTAAGCTAGAGGACACCCAAGTTTTAAATGATGTTGAACTAGATGACATCAAGAAACTAGAAGAGTTGAAAAAAGAATTGGAAAACACCTTTGATCCACGTACCAGAATTGAAATTAAACGTGAAATCAAAGAGTTGGAACGTAAACTAAGAAGGAACCGTTAG", "aa": "MSKDKKNKVEQLEPVDLFERTKLEDTQVLNDVELDDIKKLEELKKELENTFDPRTRIEIKREIKELERKLRRNR", "aa_hexdigest": "b2fb131c574bc736a14e56dd6c917ad9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63C9", "ncbi_nrp_id": "WP_014325500.1", "uniref100_id": "UniRef100_A0AAX0SQJ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63C9", "RefSeq:WP_014325500.1", "UniRef:UniRef100_A0AAX0SQJ9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQJ9", "uniref90_id": "UniRef90_Q9EXD3" }, "psc": { "uniref90_id": "UniRef90_Q9EXD3", "product": "Uncharacterized protein MPN_377", "uniref50_id": "UniRef50_Q9EXD3" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD3" ], "product": "Uncharacterized protein MPN_377" }, "genes": [], "id": "CNOCLNGIAL_821", "locus": "GCA000143945_00799" }, { "type": "cds", "sequence": "contig_1", "start": 451794, "stop": 452330, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTAACCTCCACACTAACTCTTACTATAACTTCCTCAACTCCACACTTTCACCGCAAAAGCTAGTTGACCTAGCTGTTCAAGACCAACAGGTAGCTGTTTGTTTAACCGATCCCAACTTATTTGGTGCTACCGAGTTTTTTTTGGCCTGTCAAAAAGCGCACATTAAACCCTTAATTGGGTTGAGTGTTACTGTTCGACATTACGAACAAAACGTTAATTTACTAGTAATTGCCCAAACCAACCGGGGTTATCAGAACCTAATGTGCTTAGCGTTAGTTAAAGACCAACCAGATTTGCAATTAGAACCCTTTTTGGATGGTAATGTGGTAATTATTTGCACACAAACTGAACTGCGCCTCAACACCGCAAACCCCGTTTACTTAGCACATGGTTTGAGTGGTCGTTATCCTAAAATTGCAGTAACACAAAAACCAGTTAAGTGTCAAAACACCAATAAGGACTTAACACTGCTCTTAACACTTAAGCAAATTAGTCAAATTAACAGCGAACACTTCCAGCCACTGGAATGA", "aa": "MFVNLHTNSYYNFLNSTLSPQKLVDLAVQDQQVAVCLTDPNLFGATEFFLACQKAHIKPLIGLSVTVRHYEQNVNLLVIAQTNRGYQNLMCLALVKDQPDLQLEPFLDGNVVIICTQTELRLNTANPVYLAHGLSGRYPKIAVTQKPVKCQNTNKDLTLLLTLKQISQINSEHFQPLE", "aa_hexdigest": "3209869950fa494f0036ef272393cc74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20022.923600000006, "isoelectric_point": 6.943928718566894 }, "id": "CNOCLNGIAL_822", "locus": "GCA000143945_00800" }, { "type": "cds", "sequence": "contig_1", "start": 452517, "stop": 453917, "strand": "+", "frame": 3, "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "COG:COG0587", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0008408", "SO:0001217", "UniRef:UniRef50_Q49405", "UniRef:UniRef90_P75404" ], "nt": "GTGTCACACAACGATGCACAAATGTTAAGCGAGCAGTGTCAACTAGCCTTAACTGAATTTTTAAAGCTCAATCCGCAATTAAACAAACAGCTCTATGAAGAGCGGTTAGCCAAAGAATTGGAGATAATTAACTCACTCCACTTTGCTGGTTACTTTTTAGTGGTGAGCGACTTAGTTCAGTTTGCCCATAATAACGATATCCTCATCGGTCCTGGTCGAGGATCTGCAGTGGGTTCTTTAGTGGCCTTTCTGCTCAAAATTACCCAAATTGATCCAGTAGCCAACAATCTCATTTTTGAGCGCTTTATTTCCCGTCACCGCCAGGGCTTACCTGACATTGACATTGACATTATGGAAACTAAACGTGATTTAGTAATTGACTATGTCATGCAAAAATATGGCCGGGAACAGTGTGCACAAATAGTTACTTTCCAAAAATTTAAGACCCGCTCTGCGCTGCGTGATGTCGGTAAGGTATTTAAACACATTGAGGGTGCTGAAGATTTACTGGGTAAACTACCAAAAGACAAAAGCTTATTGGAGCTTGATGTAAACGGTGTTACTGATCCGGTTTTACAACTTAGTTTAAAGAGCTTTCGCTTGTTGTGGGAGGTGGCGCGTGAAATTATTAACTTTCCGCGCCAACCAAGCATTCACGCTTCTGGTGTTGTGATTGTCACTGAACCTTTAATTACCACGATCCCTTTAATGGTGGGTAATAACAACAACTACGTCACCCAAGTGTCGATGGACTGGTTAGAATGGTACAACCTCAATAAGTTTGACTTATTGGGTTTAATTAACCTCACCATGATCCATGAGGTTGTGCAAGCGGTAAAACCCAAGGAAGTTAGCGTACAGCAATTCCTCCAACAAATACCGCTAGATGATGAAGCTACTTTTACTAACCTCACTAACCAAGCCACTTTAGGTGTTTTTCAACTAGAGTCGTTTGGGATGAAAAAGGTGCTCAAACAAATTAAACCGCACAACCTCCATGATTTAGCAATTGTTTTAGCACTTTACCGCCCCGGTCCACAGGACAACATTAATACCTTTATTGCTAACCGTAACTTAGGGTTTGACACATCAGACATCGATCCGCGAATTTTGCCAATTCTCAAAGAAACTTACGGAGTGTTAATCTTTCAAGAACAGGTTATTAACATCGCCAAAACTGTTGCTAACTATTCATTGGAAACCGCTGATAGCTTTCGCCGTGCCATTTCCAAAAAAAACTTGCAAGTAATTCAGGACAACATGCGCAGCTTTTATGAAGGCGCGCTAGCCAATAACTTTAGCTTAAAAGCAGCGACTACTATCTTTAATTACATCCAGCGCTTTGCTGGATATGGTTTTAACTTGTCCCACGCCTTGGGCTATGCTTTATTGAGTTACTGA", "aa": "MSHNDAQMLSEQCQLALTEFLKLNPQLNKQLYEERLAKELEIINSLHFAGYFLVVSDLVQFAHNNDILIGPGRGSAVGSLVAFLLKITQIDPVANNLIFERFISRHRQGLPDIDIDIMETKRDLVIDYVMQKYGREQCAQIVTFQKFKTRSALRDVGKVFKHIEGAEDLLGKLPKDKSLLELDVNGVTDPVLQLSLKSFRLLWEVAREIINFPRQPSIHASGVVIVTEPLITTIPLMVGNNNNYVTQVSMDWLEWYNLNKFDLLGLINLTMIHEVVQAVKPKEVSVQQFLQQIPLDDEATFTNLTNQATLGVFQLESFGMKKVLKQIKPHNLHDLAIVLALYRPGPQDNINTFIANRNLGFDTSDIDPRILPILKETYGVLIFQEQVINIAKTVANYSLETADSFRRAISKKNLQVIQDNMRSFYEGALANNFSLKAATTIFNYIQRFAGYGFNLSHALGYALLSY", "aa_hexdigest": "8a73364633c7a100d65f234a08b5fac2", "start_type": "GTG", "rbs_motif": null, "genes": [ "dnaE" ], "truncated": "both", "pseudogene": { "start": 452217, "stop": 454220, "observations": { "mutation": [ 452328, 452481, 453915, 453924, 453963 ], "stop": [ 452328, 452481, 453915, 453924, 453963 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75404", "query_cov": 1.0, "subject_cov": 0.5344036697247706, "identity": 0.9940000000000001, "score": 906.0, "evalue": 0.0, "gene_start": 1, "gene_end": 466, "reference_start": 242, "reference_end": 707, "reference_sequence": "MFVNLHTNSYYNFLNSTLSPQKLVDLAVQDQQVAVCLTDPNLFGATEFFLACQKAHIKPLIGLSVTVRHYEQNVNLLVIAQTNRGYQNLMCLALVKDQPDLQLEPFLDGNVVIICTQTELRLNTANPVYLAHGLSGRYPKIAVTQKPVKCQNTNKDLTLLLTLKQISQINSEHFQPLEWKLSRSLNEIQLDPPLLQQLRHQPFLSQKAAQQIFSEEELGNLQKLVEQSQWDLTKLKASSLQVSHNDAQMLSEQCQLALTEFLKLNPQLNKQLYEERLAKELEIINSLHFASYFLVVSDLVQFAHNNDILIGPGRGSAVGSLVAFLLKITQIDPVANNLIFERFISRHRQGLPDIDIDIMETKRDLVIDYVMQKYGREQCAQIVTFQKFKTRSALRDVGKVFNHIEGAEDLLGKLPKDKSLLELDVNGVTDPVLQLSLKSFRLLWEVAREIINFPRQPSIHASGVVIVTEPLITTIPLMVGNNNNYVTQVSMDWLEWYNLNKFDLLGLINLTMIHEVVQAVKPKEVSVQQFLQQIPLDDEATFTNLTNQATLGVFQLESFGMKKVLKQIKPHNLHDLAIVLALYRPGPQDNINTFIANRNLGFDTSDIDPRILPILKETYGVLIFQEQVINIAKTVANYSLETADSFRRAISKKNLQVIQDNMRSFYEGALANNFSLKAATTIFNYIQRFAGYGFNLSHALGYALLSYWTAWLKTHYFEQFNLWWLNHEQGKKEKQKQLLNEFISSGYEICPPLINKAKSDFSVQDKKLYLGFKLINGIGDKQAHALEHVQEVLKQNPNLSLIATVNLCLSKTVGGLELKDITLLQQAGCFNCFNYTVDFNLAKSFWVQSNHELFPKIPLDQPPVINWKSFGF" }, "paralog": false, "identity": 0.9895052473763118, "score": 1289.0, "evalue": 0.0, "description": "Internal stop codon at 452328, 452481, 453915, 453924, 453963. Nonsense mutation around 452328, 452481, 453915, 453924, 453963.", "psc": { "uniref90_id": "UniRef90_P75404", "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q49405", "cog_id": "COG0587", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75404", "EC:2.7.7.7", "UniRef:UniRef50_Q49405", "COG:COG0587", "COG:L", "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q49405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49405" ], "product": "DNA polymerase III subunit alpha" } }, "id": "CNOCLNGIAL_823", "locus": "GCA000143945_00801" }, { "type": "cds", "sequence": "contig_1", "start": 454149, "stop": 454394, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACATGTTCAGGAAGTTTTAAAGCAAAACCCCAATTTATCCTTAATAGCTACCGTTAATTTGTGTTTGAGCAAAACGGTAGGGGGTTTAGAACTTAAAGACATTACATTGCTCCAACAAGCAGGTTGTTTTAATTGCTTTAATTACACGGTTGACTTTAATTTGGCCAAAAGCTTTTGGGTGCAAAGTAACCATGAATTATTTCCAAAAATACCACTAGATCAACCACCCGTAATTAACTGA", "aa": "MEHVQEVLKQNPNLSLIATVNLCLSKTVGGLELKDITLLQQAGCFNCFNYTVDFNLAKSFWVQSNHELFPKIPLDQPPVIN", "aa_hexdigest": "014008d0d84c6781f1abcd1553ee6881", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9129.473399999993, "isoelectric_point": 5.453553581237792 }, "id": "CNOCLNGIAL_824", "locus": "GCA000143945_00802" }, { "type": "cds", "sequence": "contig_1", "start": 454728, "stop": 455291, "strand": "+", "frame": 3, "gene": "polA", "product": "5'-3' exonuclease", "db_xrefs": [ "COG:COG0258", "COG:L", "EC:3.1.11.-", "GO:0003677", "GO:0008409", "GO:0017108", "GO:0033567", "SO:0001217", "UniRef:UniRef50_P75403", "UniRef:UniRef90_P75403" ], "nt": "GTGTGTGAACAAGACGGGTTTGAGGCAGATGATCTAATTGGTAGTTATGCGAGGTTAATGACACAAAACAATGTGGCAGTAGACATCTACAGCTCTGACCGTGATTTACTACAGTTAGTCGATAGTATGACTAGTGTTTGGCTTTGTGTAAAGGGCACAAAAGAGATGAAGGAATATAACACTGACAACTTTGCTGAGCAGTTTTTTGGCTTAACACCAAAACAAGTGATTGAATATAAAGGTTTGGTAGGGGATAACAGTGATAACCTCACTGGCATTAAGGGAATTGGTCCGAAAAAAGGGATTGATCTACTCAAACAATACGGTACCATTGACAATATTTTTGCTAACTTTGACAAGCTATCCAAAGCATTGCAAACGATTCTCCAAGGCCAAATTGACACTGCTAAGAAATTCAGCTTTTTAGCGAGCATTAAAACAGATATTAAACTTAATGACGATATTGTCCACGCTGCTTTAAAACCAATCGATAAACAAGCTTTATTAGAGCTTTTAGATAAATATGGCATTAAAGCACTAGCGCAAAAATTTAGTCAACTGTAA", "aa": "MCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEMKEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTAKKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL", "aa_hexdigest": "daef5f8d5d43e87d2333fe85ad5f3a91", "start_type": "GTG", "rbs_motif": null, "genes": [ "polA" ], "truncated": "5-prime", "pseudogene": { "start": 454428, "stop": 455291, "observations": { "mutation": [ 454476, 454551 ], "stop": [ 454476, 454551 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75403", "query_cov": 1.0, "subject_cov": 0.6426116838487973, "identity": 0.995, "score": 369.0, "evalue": 8.07e-128, "gene_start": 1, "gene_end": 187, "reference_start": 105, "reference_end": 291, "reference_sequence": "MKNAILIDGNSLAYRAYFATWQQVEFAKLHNLPFNNAIRTMLMMCWNLLQSKQYDYGVISFDTKAPTFRDQLYSEYKSKRSKTPSELLVQIPVVKESLRHLGFLVCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEMKEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTAKKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL" }, "paralog": false, "identity": 0.9930313588850174, "score": 560.0, "evalue": 2.38e-205, "description": "Internal stop codon at 454476, 454551. Nonsense mutation around 454476, 454551.", "psc": { "uniref90_id": "UniRef90_P75403", "gene": "polA", "product": "5'-3' exonuclease", "ec_ids": [ "3.1.11.-" ], "uniref50_id": "UniRef50_P75403", "cog_id": "COG0258", "cog_category": "L", "go_ids": [ "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75403", "EC:3.1.11.-", "UniRef:UniRef50_P75403", "COG:COG0258", "COG:L", "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ] }, "pscc": { "uniref50_id": "UniRef50_P75403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75403" ], "product": "5'-3' exonuclease" } }, "id": "CNOCLNGIAL_825", "locus": "GCA000143945_00803" }, { "type": "cds", "sequence": "contig_1", "start": 455291, "stop": 456124, "strand": "+", "frame": 2, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "COG:COG0266", "COG:L", "EC:3.2.2.23", "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078", "KEGG:K10563", "RefSeq:WP_010874736.1", "SO:0001217", "UniParc:UPI000012AB8D", "UniRef:UniRef100_P75402", "UniRef:UniRef50_P55825", "UniRef:UniRef90_P75402" ], "nt": "ATGCCTGAACTACCTGAAGTTGCCACTGTAATTACCGAACTTAAGAGCTGTGTTTTAAACAAACCTGTCAAGCAAGTTAAGGTGCACTTAGACAAGGTGTTGAAAAATACTAATGTCAAGCAGCTTAACGATGCGTTAGTCAACCACAGCTTTGTGGACATTAAGCGCCGCGGCAAGTACATTATTTTTTGTTTAAGTAACGGTTTGTTTTTAGTGTCACACTTACGCATGGAAGGCAAATATTTCTTTGAAGCTAAAGGCAGTCAGTTTGACTTAAACCATGTTTTAGTCGAGTTTTTATTCCAAGATGGTGATCAACTTAACTATCATGATACGCGTCAATTTGGTACCTTTCACTTGTTCAATAGATATCAGTTTGAAAACGCACGTGAATTGAACAAATTAGCACTCGATCCCTTAGATCAGGAATTTAACCATCAAGCGATCTTTAACAAAGGCCATAAGTCCAACAAGAAGATTAAAACCTTTATTTTAGATCAGACCAACATTAGCGGTATTGGCAATATTTACGCTGATGAAATTTTGTTTGCAAGTAAGATTCACCCTGAAACTTTAGCTAAAAATTTGAACTTATCGCAGTATCAGCTAATTTGTCAAAATGCAACTGATATTTTAAAGAAAGCAGTTGAAATGAAGGGTACTACCATTGGCACTTTTACCTTTAAAAAAGATCATACCGGTGGTTACCAACACTTCTTGAAAATTCATGGCAAAAAGGGGAAGCAATGTCAAAGCTGTAACACCACTATTATTAAGAAAAAGATTAATGGTCGGGGCAGCTATATTTGTGAAAAGTGTCAAATACAGCGTTAA", "aa": "MPELPEVATVITELKSCVLNKPVKQVKVHLDKVLKNTNVKQLNDALVNHSFVDIKRRGKYIIFCLSNGLFLVSHLRMEGKYFFEAKGSQFDLNHVLVEFLFQDGDQLNYHDTRQFGTFHLFNRYQFENARELNKLALDPLDQEFNHQAIFNKGHKSNKKIKTFILDQTNISGIGNIYADEILFASKIHPETLAKNLNLSQYQLICQNATDILKKAVEMKGTTIGTFTFKKDHTGGYQHFLKIHGKKGKQCQSCNTTIIKKKINGRGSYICEKCQIQR", "aa_hexdigest": "d964a67a07a0bb109692ac793c81463c", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI000012AB8D", "ncbi_nrp_id": "WP_010874736.1", "uniref100_id": "UniRef100_P75402", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012AB8D", "RefSeq:WP_010874736.1", "UniRef:UniRef100_P75402" ] }, "ips": { "uniref100_id": "UniRef100_P75402", "uniref90_id": "UniRef90_P75402" }, "psc": { "uniref90_id": "UniRef90_P75402", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23" ], "uniref50_id": "UniRef50_P55825", "cog_id": "COG0266", "cog_category": "L", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ] }, "pscc": { "uniref50_id": "UniRef50_P55825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55825" ], "product": "Formamidopyrimidine-DNA glycosylase" }, "genes": [ "mutM" ], "id": "CNOCLNGIAL_826", "locus": "GCA000143945_00804" }, { "type": "cds", "sequence": "contig_1", "start": 456444, "stop": 456647, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "GTGACTGGCCGTGATTACTGTCGCGTTCGTTGGTTAGAAAAGTACCTAAACACTGATCTACCAACAATTACCCTCGATGGCGCCATTATTTACTTCCGCGATGAAATTATTCGGCAAGAAGTGCTTGATAAGGAATTTATCCATGGCATTAACCAGATTGTCAAACGTTACCCTACAGCAGCCTTTAAGCTCAACATGGGTTGA", "aa": "MTGRDYCRVRWLEKYLNTDLPTITLDGAIIYFRDEIIRQEVLDKEFIHGINQIVKRYPTAAFKLNMG", "aa_hexdigest": "10eb145111f806d704660ca97303c7db", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 58, "aa_cov": 0.8507462686567164, "hmm_cov": 1.0, "evalue": 1.8220848059208909e-07, "score": 26.570499420166016, "start": 2, "stop": 58 } ], "seq_stats": { "molecular_weight": 7964.164399999998, "isoelectric_point": 7.879986763000487 }, "id": "CNOCLNGIAL_827", "locus": "GCA000143945_00805" }, { "type": "cds", "sequence": "contig_1", "start": 456777, "stop": 457025, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAAAGTAAATCAGGTTTACTTTGACACATTTACTTGCCCTGAACCAATGATCCAAGAGCTCGATAACTTAGTTGAAAAAAGTGATGTAACCGCCAAAAGCTACCGCCATTCACTTTACATTATTAAAAAAGGGGTATCGAAGGCTAGTGCACTACAAAACCTCCAACGAGACTTTTTAGTGGAAATGAAGCCTGCTAACACGATTGTCTTTGGTGATGGGGATAATGACATTGAAATGATGCAGTGA", "aa": "MKVNQVYFDTFTCPEPMIQELDNLVEKSDVTAKSYRHSLYIIKKGVSKASALQNLQRDFLVEMKPANTIVFGDGDNDIEMMQ", "aa_hexdigest": "39b9727459c323d7ef78bc4b7d0bf32e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 68, "aa_cov": 0.8292682926829268, "hmm_cov": 0.9705882352941176, "evalue": 3.9565042273746534e-14, "score": 48.40382766723633, "start": 15, "stop": 82 } ], "seq_stats": { "molecular_weight": 9389.674399999998, "isoelectric_point": 4.93211269378662 }, "id": "CNOCLNGIAL_828", "locus": "GCA000143945_00806" }, { "type": "cds", "sequence": "contig_1", "start": 457038, "stop": 457148, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCGTTAACTGGTAGTGATCCCGAGTGTTATAAGTTAGCTAAGTACCACACCGACAGCGTTGACGATGATGGGATTGCCAAATGGATTAATAAAAATCTGTTATGTTAA", "aa": "MSLTGSDPECYKLAKYHTDSVDDDGIAKWINKNLLC", "aa_hexdigest": "5b405e6f40582844bc0e46a4ed34cd66", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4045.5279999999993, "isoelectric_point": 4.822015571594237 }, "id": "CNOCLNGIAL_829", "locus": "GCA000143945_00807" }, { "type": "cds", "sequence": "contig_1", "start": 457142, "stop": 457420, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01121.26" ], "nt": "ATGTTAATCGCTGTTGTAGGCAAAGCTGGAGTTGGTAAAACCACCGTTTTGCAATACATCGCTGACTATTTTCATTTTCCCGTTTTTTTTGCTGATCGGTTTATCCATCAACAATACGCAAATGGTCAAGCTGGTTATGCAATTGTAAAACAGCAATTTGGTGCTCAGTTTGTCAATCATGAAGCAGTTGATCGCAAACAGTTAGCACAATACGTTTTTAATCAACCTGATGAGTTAAAGCGTTTGTCCAACTTAACCAAACCCCTAGTACAAGAGTGA", "aa": "MLIAVVGKAGVGKTTVLQYIADYFHFPVFFADRFIHQQYANGQAGYAIVKQQFGAQFVNHEAVDRKQLAQYVFNQPDELKRLSNLTKPLVQE", "aa_hexdigest": "7443c091d42745058c1f67b8fe99bff9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01121.26", "name": "CoaE", "length": 92, "aa_cov": 0.9891304347826086, "hmm_cov": 0.9891304347826086, "evalue": 3.2324029702743695e-14, "score": 48.453372955322266, "start": 1, "stop": 91 } ], "seq_stats": { "molecular_weight": 10475.864699999995, "isoelectric_point": 9.004637718200684 }, "id": "CNOCLNGIAL_830", "locus": "GCA000143945_00808" }, { "type": "cds", "sequence": "contig_1", "start": 457674, "stop": 458522, "strand": "-", "frame": 1, "gene": "cof", "product": "Putative phosphatase MPN_383", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "RefSeq:WP_010874739.1", "SO:0001217", "UniParc:UPI0000139536", "UniRef:UniRef100_P75399", "UniRef:UniRef50_P75399", "UniRef:UniRef90_P75399" ], "nt": "ATGAAACCAAAAGTACAAAACTTAATTTTTGACCTCGATGGTACTTTATTGTCTTGGGGTCATGAACCACTACCACAAACTGTTACTTTTCTCAAAGAGTTGCAAAAGCAGGGGTTTAAAATTACCTTTGCGACTGGGCGCAGTCATATCTTAATCCGCAATACCACTCAGTTTATTCAACCTGATCTACCGGTTATTTCCTCTAACGGTGCTTTAATTTATGACTTTGCTCGGGAAAAAGCGCTGCATATGACCCAGTTAGCACCCCAAAGTGTGGTACCCATTATGCGTTTACTGTTGCAATTGGAAGAAAGCTTTTGCATTTACACTGATAAAAAGGTGTTTGGTTTTGAAAAACCCGGTATACCTTGCAAACGGTTACGCACTACTCAAAGCAAAATTGTAGAACCTGACATTACACAAAATAACTTTACCATTAATCCGTTAACTGATGCTAGCAAGTTTGATTTTGCCACACAAAACATTACCAAGATTCTTTTAATCACTGAAGACAGGGGTCGCATTAGTAAAATAACCAAACATCTCGATGCGATTGAAAACATTAGCTATGTCAGTTCTATGACCTTTGCGCTTGACATTATGCACAAAGATGTCAATAAGGCATACGGTCTCAAAGCATTAGAACAGCAAACCGGTTTAGATCCCCAAATGACCATGGTCTTTGGCGATGGGGACAATGATGTGGAAATTTTTAATGCTGTCAAGTACTCTGTCGCAATGGCTAACGGTAGTGATTTAGCAAAACAAAACGCGACCTTTATTTCGGAGTTTGATAATGACCATGATGGCATTTATCACTTTTTGCAATGCTTTCTAAAAATTGAGTAA", "aa": "MKPKVQNLIFDLDGTLLSWGHEPLPQTVTFLKELQKQGFKITFATGRSHILIRNTTQFIQPDLPVISSNGALIYDFAREKALHMTQLAPQSVVPIMRLLLQLEESFCIYTDKKVFGFEKPGIPCKRLRTTQSKIVEPDITQNNFTINPLTDASKFDFATQNITKILLITEDRGRISKITKHLDAIENISYVSSMTFALDIMHKDVNKAYGLKALEQQTGLDPQMTMVFGDGDNDVEIFNAVKYSVAMANGSDLAKQNATFISEFDNDHDGIYHFLQCFLKIE", "aa_hexdigest": "1ad92bee8a24b5640789e142d243a3bb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139536", "ncbi_nrp_id": "WP_010874739.1", "uniref100_id": "UniRef100_P75399", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139536", "RefSeq:WP_010874739.1", "UniRef:UniRef100_P75399" ] }, "ips": { "uniref100_id": "UniRef100_P75399", "uniref90_id": "UniRef90_P75399" }, "psc": { "uniref90_id": "UniRef90_P75399", "gene": "Cof", "product": "Putative phosphatase MPN_383", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P75399", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P75399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75399" ], "product": "Putative phosphatase MPN_383" }, "genes": [ "cof" ], "id": "CNOCLNGIAL_831", "locus": "GCA000143945_00809" }, { "type": "cds", "sequence": "contig_1", "start": 458525, "stop": 459040, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "GTGCACAATTTCTTTCACAAGGAAAACATTATTAAAGAAACGGTAGATCAAGAAACTGTTTATGGTTATAACCTCTTTTTAAAGCGTAGTTACGAGCACTTAGAGAAACAAGAACTCAATTTGGTAATTAGCCAAATGATGATCTTTTTAAACCTGCTTTACAAAACAAAACAATTAACCCTGGCTTATGCAAAGGGCTTTTTGACGGTGTTATCGTTTTTTGCACCTTATTTAGCGGAAGAATTAAACGCTAAACTAGGGATGGAGCCGTTTATTGTTCATGCTCAACTACCAAATGTTGATAACAGCGTACTTGAAACCGATAAGGTTAAAATTATTCTTTCGGTCAACGGTCGCTTCAAAGGTACCAAGGAATTTGCTAAAGGGGTTGATGAACAGACTGTCTTAAAAGCCTTTAAGACAGATCCGGAATTCCAAGCGTTGTTCGACCAACCCTTAGCGCGTGTTGTTTTTGTCCCTAATCGTATTATTAATGTTCTTTTAAAATCCGAATAG", "aa": "MHNFFHKENIIKETVDQETVYGYNLFLKRSYEHLEKQELNLVISQMMIFLNLLYKTKQLTLAYAKGFLTVLSFFAPYLAEELNAKLGMEPFIVHAQLPNVDNSVLETDKVKIILSVNGRFKGTKEFAKGVDEQTVLKAFKTDPEFQALFDQPLARVVFVPNRIINVLLKSE", "aa_hexdigest": "e305be601141d12026cba9449c168731", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 143, "aa_cov": 0.672514619883041, "hmm_cov": 0.993006993006993, "evalue": 3.316742157200827e-12, "score": 41.97935104370117, "start": 20, "stop": 134 } ], "seq_stats": { "molecular_weight": 19760.796199999993, "isoelectric_point": 6.921022605895996 }, "id": "CNOCLNGIAL_832", "locus": "GCA000143945_00810" }, { "type": "cds", "sequence": "contig_1", "start": 459083, "stop": 459520, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGAAACAAGGTAATCAAACTTTTACTCGGGAAACGAGCACTATGCCCCAGTGGGCTGGTTCTTGTTGGTATTACTTGGGTTATTTGATGTTAATTAATAACGAAAACTTTTGGCCAATTGACTCCAAGGAAGCGAAAGATCTTTTTGAGCGTTACCTACCAGTGGATCTGTATGTGGGTGGTGCCGAACATGCCGTGTTGCACCTCTTGTACGCGCGCTTTTGGCACCAATTCTTGTACCAAAAGGGAATAGTCACCACTAAAGAGCCCTTTAAAAAGTTAATTAACCAGGGCATGGTATTGGGACCAGATGGCAAAAAGATGTCCAAATCCAAGGGCAACACAATCAACCCTACGCCACTAATTGATTCTCATGGTGCTGATGCCTTGCGACTTTACTTGATGTTTATGGGGCCGATTAGCGCGGCGTTGACCTGA", "aa": "MKQGNQTFTRETSTMPQWAGSCWYYLGYLMLINNENFWPIDSKEAKDLFERYLPVDLYVGGAEHAVLHLLYARFWHQFLYQKGIVTTKEPFKKLINQGMVLGPDGKKMSKSKGNTINPTPLIDSHGADALRLYLMFMGPISAALT", "aa_hexdigest": "fa35e8e3f6bbdb15f8ff6a03f6f758ff", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0012B0E3D9", "query_cov": 0.8551724137931035, "subject_cov": 0.9323308270676691, "identity": 0.621, "score": 157.0, "evalue": 7.79e-47, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A359BCU9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 138, "aa_cov": 0.9448275862068966, "hmm_cov": 0.8695652173913043, "evalue": 4.9569185092252584e-20, "score": 67.05760192871094, "start": 3, "stop": 139 } ], "seq_stats": { "molecular_weight": 16568.020999999982, "isoelectric_point": 8.946422767639163 }, "id": "CNOCLNGIAL_833", "locus": "GCA000143945_00811" }, { "type": "cds", "sequence": "contig_1", "start": 459542, "stop": 460246, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF13603.12" ], "nt": "ATGCAAACCAACTGGATTGGTAAAAGTGAAGGGGTAGAGGTAACGTTTGACATAGAAAACTCCAAGGAAACGATTACCATTTTTACCACTAAGGTGCAAACTATTTTTGGTGTAACCTTTTTAGCGCTTTCCACTAGCCATCCCTTAGTAAAAGAGGTAGCTAAAACCAACCCCAAGATAGCACAGTTTCTCCAAAAACAAGCACAGAAAACCACCACTGTAAAGCAACCTGAGACGCTCCATGACGGGGTTGATTTAAAACTTAAAGCAATTAATCCAGCAACTAATACTGCCATTCCGTTGTATGTAGCTAACTATGTAGTGGAAGGTTATGGTACGGATGCTGTGATGGGTGTGCCAGCGCATAACGAAAACGATAACTTCTTCGCGCGGAAGCAAAAGCTACCGATTATTACCGTCATTGATAAACAGGAACGCTTGCAACACTCCGGGCAGTTTAGCGGGCTCAACTCCCAAACTGCTAACACCCAAATTACGCAAATGTTAGTCGAGCGTCAAAAAGCTAAAAAAACAACGGTGTACAAACTCCGTGACTGGATTTTTAGCCGTCAACGTTACTGGGGTGAACCTTTTCCGATTTTGTTTGACGAAAACAACCAACCCCATTTAGTTAAAGAGCTCCCCGTTACCCTACCAGCTTTGGCTAACTATCAACCTGATGGTTCGACTAACCCACCATTGTGA", "aa": "MQTNWIGKSEGVEVTFDIENSKETITIFTTKVQTIFGVTFLALSTSHPLVKEVAKTNPKIAQFLQKQAQKTTTVKQPETLHDGVDLKLKAINPATNTAIPLYVANYVVEGYGTDAVMGVPAHNENDNFFARKQKLPIITVIDKQERLQHSGQFSGLNSQTANTQITQMLVERQKAKKTTVYKLRDWIFSRQRYWGEPFPILFDENNQPHLVKELPVTLPALANYQPDGSTNPPL", "aa_hexdigest": "7b7a2d5b566bfd2bacbf2855331d8ea3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 39, "aa_cov": 0.16666666666666666, "hmm_cov": 1.0, "evalue": 1.4741982841079085e-07, "score": 25.971345901489258, "start": 170, "stop": 208 }, { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 180, "aa_cov": 0.6965811965811965, "hmm_cov": 1.0, "evalue": 1.1899602668903198e-54, "score": 180.0886688232422, "start": 4, "stop": 166 } ], "seq_stats": { "molecular_weight": 26338.714400000004, "isoelectric_point": 8.942103385925293 }, "id": "CNOCLNGIAL_834", "locus": "GCA000143945_00812" }, { "type": "cds", "sequence": "contig_1", "start": 460265, "stop": 460462, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGGAGATTGATGTCAACTGGTGTGCTGAATTGGGCACCGTTTTAGCCAACGAAGAGGTGTTGATTGACAGTAATGGTAATGCGGTGAGTGAGCGTGGCGAGTTCCCGGTAACCAAGCGAAAAATGAAGCAATGGGTGTTGAAAATAACTGCTTTTGCTGAAAGTTTACTCAATGGTTTGGCTGAGCTGGATTGA", "aa": "MKEIDVNWCAELGTVLANEEVLIDSNGNAVSERGEFPVTKRKMKQWVLKITAFAESLLNGLAELD", "aa_hexdigest": "2ab89cd3ebffe9460b2bc0c35556143d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7236.2179, "isoelectric_point": 4.67252902984619 }, "id": "CNOCLNGIAL_835", "locus": "GCA000143945_00813" }, { "type": "cds", "sequence": "contig_1", "start": 460613, "stop": 460798, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTCCCTTATCCTTCAGCGGCTGGTTTACACCTGGGACATGTGCGCGCTTACACCATTACCGATGTTTTGAGTCGTTATCATAAAGCGAAGGGTTTTAATGTGATTCACCCGATTGGCTTTGATGCTTTTGGGTTACCAGCAGAACAATACGCGATTGCCTCCAATAAGCATCCTGGTGACTGA", "aa": "MFPYPSAAGLHLGHVRAYTITDVLSRYHKAKGFNVIHPIGFDAFGLPAEQYAIASNKHPGD", "aa_hexdigest": "f22d21b9d83767ebec682861ab446d36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.7540983606557377, "hmm_cov": 1.0, "evalue": 1.2275630192374192e-09, "score": 32.821102142333984, "start": 5, "stop": 50 } ], "seq_stats": { "molecular_weight": 6666.495200000001, "isoelectric_point": 8.155846977233889 }, "id": "CNOCLNGIAL_836", "locus": "GCA000143945_00814" }, { "type": "cds", "sequence": "contig_1", "start": 460907, "stop": 461026, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCACTTCTTTATTCCAGTTGGTTTTGGTTTACTTTTTGGGATTGCTGTAGCCATTATAGCTGACCTGTTCTACCATTTAATTAAGTATTTAATTAAGCGCTCGTTCAAAAAAAACTAG", "aa": "MHFFIPVGFGLLFGIAVAIIADLFYHLIKYLIKRSFKKN", "aa_hexdigest": "ab688bbc77cdb5775449223d56b3321a", "start_type": "GTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4542.519700000001, "isoelectric_point": 9.997515296936037 }, "id": "CNOCLNGIAL_837", "locus": "GCA000143945_00815" }, { "type": "cds", "sequence": "contig_1", "start": 461072, "stop": 461251, "strand": "-", "frame": 2, "gene": null, "product": "MPN385 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47509", "UniRef:UniRef90_P75397" ], "nt": "ATGCTGCTTAAACGGCTCATTAAACTAGCGATTTTCCTCTTCTTTGTCGCGGTTGGTTTTATTATTTTTATTGGCTCTTTTTGGCTCAATACCTACAATACTAAGGAGTGGGCTAATTTACTAGCTGAAAAGGATGCTAGTGGGCTAATTGTGCAAATTATTCCCAATATTAATCAATGA", "aa": "MLLKRLIKLAIFLFFVAVGFIIFIGSFWLNTYNTKEWANLLAEKDASGLIVQIIPNINQ", "aa_hexdigest": "9fb11d38dc51af3270ccbf0b7f3f43b8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 460899, "stop": 461251, "observations": { "deletion": [ 460915 ], "mutation": [ 461074 ], "stop": [ 461074 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75397", "query_cov": 1.0, "subject_cov": 0.5042735042735043, "identity": 1.0, "score": 114.0, "evalue": 8.39e-32, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MLLKRLIKLAIFLFFVAVGFIIFIGSFWLNTYNTKEWANLLAEKDASGLIVQIIPNINQWFKGTVEQQQKLFQTLVHFFIPVGFGLLFGIAVAIIADLFYHLIKYLIKRSFKKKLDV" }, "paralog": false, "identity": 0.9914529914529915, "score": 223.0, "evalue": 7.79e-77, "description": "Internal stop codon at 461074. Frameshift due to deletion around 460915. Nonsense mutation around 461074.", "psc": { "uniref90_id": "UniRef90_P75397", "product": "MPN385 family protein", "uniref50_id": "UniRef50_P47509", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75397", "UniRef:UniRef50_P47509", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47509", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47509" ], "product": "Uncharacterized protein MG267" } }, "id": "CNOCLNGIAL_838", "locus": "GCA000143945_00816" }, { "type": "cds", "sequence": "contig_1", "start": 461286, "stop": 461654, "strand": "-", "frame": 1, "gene": "dck", "product": "putative MG268-like protein", "db_xrefs": [ "COG:COG1428", "COG:F", "GO:0005524", "GO:0005737", "GO:0019136", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_P75396" ], "nt": "TTGTTCGCCAAGCAAAACATCCACCGTCCTTCCATCTTTACTTACTATAACCACTTGTGGAATGGGATTGTTAAAGAACTAATCTTTAAACATGGTATTCCCGCTTTGTACGTTATCTTAGAAGGTGATTGGGAGTTGTTTGAACAAAGATTGTTTCAACGTAACCGTAAAGTAGAAATTGATAACTTTGCCAAAAATAAGGACTACTTTAAGAACCTGTACAAGATTTATGGTGAATTTATTAAAAACGTTTGTTATGACTTTGGTATTAGCCATTGCATAGTAAACGCTAACCAATCAGTAGAATCCATCACCAAACAAGTACTAGAGGTATTAAAGTCAAAAAACTTAGACTGGGAAATTATCTAG", "aa": "MFAKQNIHRPSIFTYYNHLWNGIVKELIFKHGIPALYVILEGDWELFEQRLFQRNRKVEIDNFAKNKDYFKNLYKIYGEFIKNVCYDFGISHCIVNANQSVESITKQVLEVLKSKNLDWEII", "aa_hexdigest": "a6fb0518e0e5df9168fc660f22ac9fea", "start_type": "TTG", "rbs_motif": null, "genes": [ "dck" ], "truncated": "3-prime", "pseudogene": { "start": 461286, "stop": 461954, "observations": { "mutation": [ 461657 ], "stop": [ 461657 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75396", "query_cov": 1.0, "subject_cov": 0.5327510917030568, "identity": 0.992, "score": 258.0, "evalue": 6.68e-86, "gene_start": 1, "gene_end": 122, "reference_start": 108, "reference_end": 229, "reference_sequence": "MKTPLKPKFQPAKIANAVVVGGMIAFGKTTIAESLAKHLKGSKVIYELEEQDQLADLLLAKMYERNDELLYAPLFQLYFTLNRFNKYRKECNNKTPTIFDRSIFEDWLFAKQNIHRPSIFTYYNHLWNGIVKELIFKHGIPALYVILEGDWELFEQRLFQRNRKVEIDNFAKNKDYFKNLYKIYGEFIKNVCYDFGISHCIVNANQSVESITKQVLEVLKSKNLDWEII" }, "paralog": false, "identity": 0.9954954954954955, "score": 449.0, "evalue": 1e-163, "description": "Internal stop codon at 461657. Nonsense mutation around 461657.", "psc": { "uniref90_id": "UniRef90_P75396", "gene": "Dck", "product": "Uncharacterized protein MG268 homolog", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0019136" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75396", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F", "GO:0005737", "GO:0005524", "GO:0019136" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "CNOCLNGIAL_839", "locus": "GCA000143945_00817" }, { "type": "cds", "sequence": "contig_1", "start": 461655, "stop": 461975, "strand": "-", "frame": 1, "gene": "dck", "product": "putative MG268-like protein", "db_xrefs": [ "COG:COG1428", "COG:F", "GO:0005524", "GO:0005737", "GO:0019136", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_P75396" ], "nt": "ATGAAGACTCCCTTAAAACCCAAATTTCAACCAGCTAAGATTGCTAACGCCGTTGTGGTTGGTGGCATGATTGCTTTTGGTAAAACGACGATAGCTGAAAGTTTAGCTAAACACTTAAAGGGTTCTAAGGTGATTTACGAACTGGAAGAACAAGACCAGCTAGCTGATTTATTGTTAGCCAAAATGTATGAGCGTAACGACGAGTTGCTCTACGCCCCTTTATTTCAACTGTACTTTACCTTAAACCGCTTTAATAAATACCGCAAGGAATGTAACAATAAAACACCAACTATCTTTGACCGCTCGATCTTTGAAGATTGA", "aa": "MKTPLKPKFQPAKIANAVVVGGMIAFGKTTIAESLAKHLKGSKVIYELEEQDQLADLLLAKMYERNDELLYAPLFQLYFTLNRFNKYRKECNNKTPTIFDRSIFED", "aa_hexdigest": "0105850491dd30c47ea318e0d7109897", "start_type": "ATG", "rbs_motif": null, "genes": [ "dck" ], "truncated": "5-prime", "pseudogene": { "start": 461352, "stop": 461975, "observations": { "mutation": [ 461657 ], "stop": [ 461657 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75396", "query_cov": 1.0, "subject_cov": 0.462882096069869, "identity": 1.0, "score": 214.0, "evalue": 5.33e-69, "gene_start": 1, "gene_end": 106, "reference_start": 1, "reference_end": 106, "reference_sequence": "MKTPLKPKFQPAKIANAVVVGGMIAFGKTTIAESLAKHLKGSKVIYELEEQDQLADLLLAKMYERNDELLYAPLFQLYFTLNRFNKYRKECNNKTPTIFDRSIFEDWLFAKQNIHRPSIFTYYNHLWNGIVKELIFKHGIPALYVILEGDWELFEQRLFQRNRKVEIDNFAKNKDYFKNLYKIYGEFIKNVCYDFGISHCIVNANQSVESITKQVLEVLKSKNLDWEII" }, "paralog": false, "identity": 0.9951690821256038, "score": 423.0, "evalue": 1.74e-153, "description": "Internal stop codon at 461657. Nonsense mutation around 461657.", "psc": { "uniref90_id": "UniRef90_P75396", "gene": "Dck", "product": "Uncharacterized protein MG268 homolog", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0019136" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75396", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F", "GO:0005737", "GO:0005524", "GO:0019136" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "CNOCLNGIAL_840", "locus": "GCA000143945_00818" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 462009, "stop": 462104, "strand": "-", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 60.8, "evalue": 1.1e-07, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "ATTTAGAGCCGTCACATCATTACGGTCGAATCATGTCAGGCCAGAAATGGAGCAGCATTAAGACTATTTAATGAGTGTGATGGTTTTTTATTGCAT", "id": "CNOCLNGIAL_42", "locus": "GCA000143945_00819" }, { "type": "cds", "sequence": "contig_1", "start": 462123, "stop": 463199, "strand": "-", "frame": 1, "gene": null, "product": "putative MG269-like protein", "db_xrefs": [ "RefSeq:WP_010874743.1", "SO:0001217", "UniParc:UPI0000139546", "UniRef:UniRef100_P75395", "UniRef:UniRef50_Q49407", "UniRef:UniRef90_P75395" ], "nt": "ATGACTAACTTTGAGTATTACCGTGATTTTGATGACTTTCAAAGAAGAGAGACAATTAACTTCTTTCTTTCAAAGTTTCCACTAGCGAGTCAAAAACAGCTCCAGGACTTCCTAGAGCAAGCGCGTCAGGCTTATGTGCAGTTACGCCAAACCAACCCTGCACACCTTGACTGGAACCAAACATTGCTGTATTTAGCACAGAAATTTCTCCCCGAAAAGCAATCAGAGAAGGATCGCTTAAAGAAGATCTTGGTGTTGCAAGAACAGCTCAAAGTACGGTATGAAGGGGAAATTAAACGCCAGAGCCAACAAAACAGTGAGCTGTTAATCCAGTTAGGTCAACGTGATGAGGAAATTATCCAGATGCAGCAGCTATTTAAAGAAAAGGAACGGCAACTAGAGGTGTATCAAAAGCAGCTCAATGAAGCCAAGGAGTACAACCACAAGCTTGAGGAACACTACAACAAAACCCTGGAAGAAGCGTTGAAGGAGTACGAGCAGCAGTGTACTGATGCCATTCACAGGCGTGATGAGGAAATCCAGGCCATCTTTACAAGTAAGTTAAACGAAAAGAACAGTGAGATTACCCAACTGCAAACTTACTTGCAAAGTGCGGTTGATGAAAATGAGGCGTTGCAAAAGCAGCACAAACTAGTGTTGTTTAAAAACCAAAAGTACGAAAAGATGGTGAGTGACTTGCAAGTCGATTTAGCCCGGATTCAGGAAATTAACAATTCACTTACCAGTGAAAAGCGTGACTTTCAGCGCGCTAACAACGATTTGGTCAAGCAGTACAACAAACTCAAAAACCGCTTGGAACAAAAGTTAGGTGAAATTACTAACGCGCAAGTTAATGGTCAGACCCATACCGTTAGCCTAGCTGACACTTCACAGCAATTCCACCGTCCTCAAGAGGCTGTTATCCCGCAAACCCAAGTTATTAGCTATACACTCGATGATATGGATGATGACATGGAAGTGGAAGAAACTCCACCAACAACCAATAAAGATCTACCTCGCGGCGCGACCCAGCCGAAGCGTAATTCGATTAAGCGCGTAAGCAAATTAATTGATTAA", "aa": "MTNFEYYRDFDDFQRRETINFFLSKFPLASQKQLQDFLEQARQAYVQLRQTNPAHLDWNQTLLYLAQKFLPEKQSEKDRLKKILVLQEQLKVRYEGEIKRQSQQNSELLIQLGQRDEEIIQMQQLFKEKERQLEVYQKQLNEAKEYNHKLEEHYNKTLEEALKEYEQQCTDAIHRRDEEIQAIFTSKLNEKNSEITQLQTYLQSAVDENEALQKQHKLVLFKNQKYEKMVSDLQVDLARIQEINNSLTSEKRDFQRANNDLVKQYNKLKNRLEQKLGEITNAQVNGQTHTVSLADTSQQFHRPQEAVIPQTQVISYTLDDMDDDMEVEETPPTTNKDLPRGATQPKRNSIKRVSKLID", "aa_hexdigest": "e68b6232f2acd9e744b6f05bfa25e0e0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139546", "ncbi_nrp_id": "WP_010874743.1", "uniref100_id": "UniRef100_P75395", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139546", "RefSeq:WP_010874743.1", "UniRef:UniRef100_P75395" ] }, "ips": { "uniref100_id": "UniRef100_P75395", "uniref90_id": "UniRef90_P75395" }, "psc": { "uniref90_id": "UniRef90_P75395", "product": "Uncharacterized protein MG269 homolog", "uniref50_id": "UniRef50_Q49407" }, "pscc": { "uniref50_id": "UniRef50_Q49407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49407" ], "product": "Uncharacterized protein MG269" }, "genes": [], "id": "CNOCLNGIAL_841", "locus": "GCA000143945_00820" }, { "type": "cds", "sequence": "contig_1", "start": 463192, "stop": 463578, "strand": "-", "frame": 3, "gene": null, "product": "putative MG269-1-like protein", "db_xrefs": [ "RefSeq:WP_014325503.1", "SO:0001217", "UniParc:UPI0001DD63CC", "UniRef:UniRef100_A0AAV5NAZ6", "UniRef:UniRef50_Q9EXD4", "UniRef:UniRef90_Q9EXD4" ], "nt": "ATGAGTAACTTCTTTGAGAAATACATTAACGGTTTTATTGAAACTTTAGACCAAATTGACGCAGCTGACTTTCAACGCATCCAACACGACTTTGACCCTAACCAGTTTCCGTATGACTGGGTGGTGGAACGGGTTAGTGATGTCAAGGATTATTTGCTCAATCCGCGTGATTTTAGCGATGTAGAAACTTTTAAAAGTACTATGCGTGCTAAGATTAAGCACTTTTATGCGTGTTATTCGAGCAAAATCCCCTTCTTTTTGTTTACTAGTTTTGTGTTAGCAATCTTTAACAGTGTTGGGCAGTATGTCAAGTACCACTGTGACTTGGACTTTACTAATCCTGATGCTGTAATTATCTTTTTCCGTGAAAAGGCCTTAAATGACTAA", "aa": "MSNFFEKYINGFIETLDQIDAADFQRIQHDFDPNQFPYDWVVERVSDVKDYLLNPRDFSDVETFKSTMRAKIKHFYACYSSKIPFFLFTSFVLAIFNSVGQYVKYHCDLDFTNPDAVIIFFREKALND", "aa_hexdigest": "89d7fb0eb7f125a51c13369f87c67d0e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63CC", "ncbi_nrp_id": "WP_014325503.1", "uniref100_id": "UniRef100_A0AAV5NAZ6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63CC", "RefSeq:WP_014325503.1", "UniRef:UniRef100_A0AAV5NAZ6" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NAZ6", "uniref90_id": "UniRef90_Q9EXD4" }, "psc": { "uniref90_id": "UniRef90_Q9EXD4", "product": "Uncharacterized protein MG269.1 homolog", "uniref50_id": "UniRef50_Q9EXD4" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD4" ], "product": "Uncharacterized protein MG269.1 homolog" }, "genes": [], "id": "CNOCLNGIAL_842", "locus": "GCA000143945_00821" }, { "type": "cds", "sequence": "contig_1", "start": 463571, "stop": 463792, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10437.14" ], "nt": "GTGCAGGTAGATCAGGGTAAAGTAGTCGACATTGAGTTCTATGGGGACTTTTTGAGTGTTATCGACATTACCCCCGTTACCCAGCAGTTGGTAGGACAAAAATACGATTACCAAATCTTTGCTAAAATATTGGGTAACATTGATAACTTTAAGGAGTACTTTGGTACCTTAACACCTCAACAAATGCTCGAAGTTATCTTTGATAACAAAAAGGATGAGTAA", "aa": "MQVDQGKVVDIEFYGDFLSVIDITPVTQQLVGQKYDYQIFAKILGNIDNFKEYFGTLTPQQMLEVIFDNKKDE", "aa_hexdigest": "eb8f8748cca5a619ac10ea17491740ef", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10437.14", "name": "Lip_prot_lig_C", "length": 66, "aa_cov": 0.9041095890410958, "hmm_cov": 0.9848484848484849, "evalue": 1.2404564035812915e-16, "score": 55.89114761352539, "start": 1, "stop": 66 } ], "seq_stats": { "molecular_weight": 8483.539199999997, "isoelectric_point": 4.293867683410644 }, "id": "CNOCLNGIAL_843", "locus": "GCA000143945_00822" }, { "type": "cds", "sequence": "contig_1", "start": 463868, "stop": 464590, "strand": "-", "frame": 2, "gene": "lplA", "product": "putative lipoate-protein ligase A", "db_xrefs": [ "COG:COG0095", "COG:H", "EC:6.3.1.20", "GO:0005524", "GO:0005737", "GO:0016979", "GO:0017118", "KEGG:K03800", "SO:0001217", "UniRef:UniRef50_P47512", "UniRef:UniRef90_P75394" ], "nt": "ATGAAAACTTACATTCTCACTTCCCCTAAAAACATTCCTTACTTCAACGCAGCGTTAGAAGAATGGCTACTCACCGAATTTAAAAAGGGCGAGGAAATTAAAGTCATTTACTTCTGGCAAAACGCCAACACGATTGTGGTGGGGCGCAACCAAAATACGTACGCTGAGGTTAACCTTAGTGAAGTGGAAAAGGACAAGGTTAATTTGTTTCGCCGTTTTTCTGGAGGTGGGGCGGTGTTTCATGACATGGGTAACATCTGTTTCTCAATTATTTTGCCCAAAGCAAAAAAGGAAATGGAAAACGCTTATGAAGAAACAACCCGTAATGTGGTGAAGTTCTTAAACAGTGTAGGTGTACCCGCACAGTTTCATGGCCGCAATGACTTGGAAATTGAGGGCAAGAAGTTCTCTGGTTTAGCGGAATACCTCTCCAAAGACCGCGTGTTGGTCCACGGTACGTTGTTGTTTGATACCGACTTTACCAAGCTAGCGAAGTACCTCAATGTGGATAAAACAAAAATGGTAAGTAAGGGAATTGAAAGTGTCCAAAAACGGGTAGTTAACGTAAAAGAATACCTCCCCAACCTCTCTACCCCAACCTTCTTGGAAAAGATGGTGCAGTTCTTTACCGAAACCGAACATGCTGAAACGATTCATTTGGATGAAAGCAGCATTAAGATGGTGGAAAAACGTGCACAGGAACACTTCCAATCCTGGGATTGA", "aa": "MKTYILTSPKNIPYFNAALEEWLLTEFKKGEEIKVIYFWQNANTIVVGRNQNTYAEVNLSEVEKDKVNLFRRFSGGGAVFHDMGNICFSIILPKAKKEMENAYEETTRNVVKFLNSVGVPAQFHGRNDLEIEGKKFSGLAEYLSKDRVLVHGTLLFDTDFTKLAKYLNVDKTKMVSKGIESVQKRVVNVKEYLPNLSTPTFLEKMVQFFTETEHAETIHLDESSIKMVEKRAQEHFQSWD", "aa_hexdigest": "35fae1dcbde01bb47762ea6eb37ab1c9", "start_type": "ATG", "rbs_motif": null, "genes": [ "lplA" ], "truncated": "5-prime", "pseudogene": { "start": 463571, "stop": 464590, "observations": { "mutation": [ 463870 ], "stop": [ 463870 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75394", "query_cov": 1.0, "subject_cov": 0.7079646017699115, "identity": 1.0, "score": 478.0, "evalue": 3.07e-169, "gene_start": 1, "gene_end": 240, "reference_start": 1, "reference_end": 240, "reference_sequence": "MKTYILTSPKNIPYFNAALEEWLLTEFKKGEEIKVIYFWQNANTIVVGRNQNTYAEVNLSEVEKDKVNLFRRFSGGGAVFHDMGNICFSIILPKAKKEMENAYEETTRNVVKFLNSVGVPAQFHGRNDLEIEGKKFSGLAEYLSKDRVLVHGTLLFDTDFTKLAKYLNVDKTKMVSKGIESVQKRVVNVKEYLPNLSTPTFLEKMVQFFTETEHAETIHLDESSIKMVEKRAQEHFQSWDWNFGKTADYNFKNKKRFEGAGIFECNVQVDQGKVVDIKFYGDFLSVIDITPVTQQLVGQKYDYQTFAKILGNIDNFKEYFGTLTPQQMLEVIFDNKKDE" }, "paralog": false, "identity": 0.9911504424778761, "score": 677.0, "evalue": 5.61e-250, "description": "Internal stop codon at 463870. Nonsense mutation around 463870.", "psc": { "uniref90_id": "UniRef90_P75394", "gene": "lplA", "product": "Probable lipoate-protein ligase A", "ec_ids": [ "6.3.1.20" ], "uniref50_id": "UniRef50_P47512", "cog_id": "COG0095", "cog_category": "H", "kegg_orthology_id": "K03800", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016979", "GO:0017118" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75394", "EC:6.3.1.20", "UniRef:UniRef50_P47512", "COG:COG0095", "COG:H", "KEGG:K03800", "GO:0005737", "GO:0005524", "GO:0016979", "GO:0017118" ] }, "pscc": { "uniref50_id": "UniRef50_P47512", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47512" ], "product": "Probable lipoate-protein ligase A" } }, "id": "CNOCLNGIAL_844", "locus": "GCA000143945_00823" }, { "type": "cds", "sequence": "contig_1", "start": 464617, "stop": 465747, "strand": "-", "frame": 3, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG1249", "EC:1.8.1.4", "GO:0004148", "GO:0005829", "GO:0006090", "GO:0006103", "GO:0016020", "GO:0050660", "KEGG:K00382", "SO:0001217", "UniRef:UniRef50_P75393", "UniRef:UniRef90_P75393" ], "nt": "TTGCTGGAGCAAAAAGGTAAGGTAGTGAGTAAGTTAGTTGGTGGGGTTAAAGCGATTATTGCTTCCGCTAAAGCTGAAACGGTAATGGGAGAAGCCAAGGTTTTGGATCCCAACACCGTGGAGGTAGCGGGTAAAACTTACACCACCAAAAGCATTGTGGTCGCAACTGGTTCACGACCCCGTTACTTAACGCTTCCGGGTTTTGCAGAAGCGCGTCAAAACGGTTTTGTAATTGACTCGACCCAAGCGCTCTCTTTGGAAGGGGTACCACGCAAGTTAGTTGTTGTTGGTGGTGGAGTGATTGGTATTGAATTTGCCTTTCTCTACGCTTCATTGGGTAGTGAAGTGACTATCTTACAAGGGGTGGATAGGATCTTGGAAATCTTTGACACTGAGGTATCAGATTTAGTCGCTAAACTCTTGCAAACCAAGAATGTCAAGATTATTACCAACGCACAGGTAACCCGTGCTAACAATAATGAAGTCTTTTATTCGCAAAACGGGCAGGAAGGCAGTGTGGTTGGTGATCGCATTTTGGTCTCGATTGGTCGCATTCCCAACACGGAATGTCTTGATGGGTTGAACTTACAACGTGATGAGCGCAACCGGATTGTGCTCAATCAAGATCTCCAAACCTCAATTCCCAACATCTACATAGTTGGCGATGCCAATGCCCAGTTAATGTTGGCCCACTTTGCCTACCAACAAGGGCGGTATGCGGTGAACCATATCTTAAACAAGAAGCAGGTCAAACCAGCGCAAAAGTTAACTTGCCCTTCCTGTATTTACACCAATCCGGAAGTAGCATCGGTGGGTTACACCGAAATGGAGTTGAAAAAACAGGGCATTCCTTATGTCAAAACCAATCTTGTTTTAGCGCACTGTGGAAAAGCAATCGCTGACAATGAAACCAACGGTTTTGTCAAAATGATGTTTGACCCACAAACCGGTAAGATCTTGGGTTGCTGTATTATTGCTGCTACTGCTAGTGACATGATTGCCGAGCTAGCGTTGGCTATGGATGCAGGTTTGACATTATTTGACATTGCCAACTCCATTTCACCACATCCTACCATCAACGAAATGATCGCTGATGTGTGCAAAAAAGCTCTTTTTGATCACTTTAAATAA", "aa": "MLEQKGKVVSKLVGGVKAIIASAKAETVMGEAKVLDPNTVEVAGKTYTTKSIVVATGSRPRYLTLPGFAEARQNGFVIDSTQALSLEGVPRKLVVVGGGVIGIEFAFLYASLGSEVTILQGVDRILEIFDTEVSDLVAKLLQTKNVKIITNAQVTRANNNEVFYSQNGQEGSVVGDRILVSIGRIPNTECLDGLNLQRDERNRIVLNQDLQTSIPNIYIVGDANAQLMLAHFAYQQGRYAVNHILNKKQVKPAQKLTCPSCIYTNPEVASVGYTEMELKKQGIPYVKTNLVLAHCGKAIADNETNGFVKMMFDPQTGKILGCCIIAATASDMIAELALAMDAGLTLFDIANSISPHPTINEMIADVCKKALFDHFK", "aa_hexdigest": "e4a0ff253e924cc8a2ff01213cf5a31c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75393", "query_cov": 1.0, "subject_cov": 0.8209606986899564, "identity": 0.997, "score": 724.0, "evalue": 3.87e-262, "valid": true, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "ec_ids": [ "1.8.1.4" ], "uniref50_id": "UniRef50_P75393", "cog_id": "COG1249", "cog_category": "C", "kegg_orthology_id": "K00382", "go_ids": [ "GO:0004148", "GO:0005829", "GO:0006090", "GO:0006103", "GO:0016020", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75393" ], "product": "Dihydrolipoyl dehydrogenase" }, "genes": [ "lpdA" ], "id": "CNOCLNGIAL_845", "locus": "GCA000143945_00824" }, { "type": "cds", "sequence": "contig_1", "start": 465754, "stop": 465990, "strand": "-", "frame": 3, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "nt": "ATGAATTACGATCTCATTATTATAGGGGCTGGTCCCGCTGGGTATGTGGCCGCTGAGTACGCGGGTAAGCATAAACTTAAAACACTCGTTGTCGAAAAGGAATACTTTGGTGGCGTGTGTTTAAATGTTGGTTGTATTCCAACGAAAACACTGTTAAAACGGGCCAAGATTGTCGATTACCTCCGCCATGCGCAGGACTATGGTATTTCAATTAATGGTCAAGTAGCACTTAACTGA", "aa": "MNYDLIIIGAGPAGYVAAEYAGKHKLKTLVVEKEYFGGVCLNVGCIPTKTLLKRAKIVDYLRHAQDYGISINGQVALN", "aa_hexdigest": "c60591b31d3004a81de8c260af0c4654", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0010C3BE0E", "query_cov": 0.9358974358974359, "subject_cov": 0.9358974358974359, "identity": 0.589, "score": 100.0, "evalue": 1.85e-26, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A7X1ZRV2" }, "pscc": { "uniref50_id": "UniRef50_A0A7X1ZRV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "CNOCLNGIAL_846", "locus": "GCA000143945_00825" }, { "type": "cds", "sequence": "contig_1", "start": 465991, "stop": 467199, "strand": "-", "frame": 3, "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "db_xrefs": [ "COG:C", "COG:COG0508", "EC:2.3.1.12", "GO:0004742", "GO:0005829", "GO:0009986", "GO:0016020", "GO:0031405", "RefSeq:WP_010874747.1", "SO:0001217", "UniParc:UPI0000130BFE", "UniRef:UniRef100_P75392", "UniRef:UniRef50_P75392", "UniRef:UniRef90_P75392" ], "nt": "ATGGCAAATGAGTTTAAGTTCACCGATGTTGGTGAGGGCTTACATGAGGGTAAAGTAACTGAAATCCTCAAAAAAGTGGGCGATACGATCAAGGTCGATGAAGCCCTTTTTGTAGTGGAAACCGATAAGGTAACCACCGAACTTCCATCCCCTTATGCTGGGGTTATTACCGCCATTACCACTAATGTTGGTGATGTGGTGCACATTGGTCAGGTGATGGCCGTTATTGATGATGGCGCTGGTGCTGCTGCACCTGCTGCGCCCCAACCAGTATCTGCACCAGCTCCTGCCCCAACCCCAACATTTACCCCAACACCAGCACCTGTAACCACCGAACCAGTAGTGGAAGAGGCGGGTGCCTCGGTAGTGGGTGAAATTAAGGTATCGAACAGTGTGTTTCCTATTTTTGGTGTGCAGCCAAGTGCGCCCCAACCAACACCAGCTCCTGTAGTACAACCTACATCCGCTCCAACTCCCACTCCAGCTCCAGCATCTGCAGCAGCACCTAGTGGTGAGGAAACGATTGCGATCACGACAATGCGTAAAGCAATTGCTGAAGCAATGGTGAAATCACACGAAAACATTCCCGCTACGATTCTCACCTTCTATGTTAACGCTACTAAGTTAAAGCAGTACCGTGAGAGTGTTAACGGCTTAGCTTTAAGCAAGTACAACATGAAGATTTCCTTCTTTGCTTTCTTTGTTAAAGCAATTGTTAATGCTTTGAAGAAGTTTCCTGTTTTCAATGGTCGTTATGACAAGGAACGCAACCTAATTGTGTTAAACAAGGATGTTAACGTAGGTATTGCCGTGGACACTCCCGATGGTCTGATTGTGCCTAACATTAAGCAAGCACAAACGAAGTCGGTAGTCGACATTGCCAAGGACATTGTTGATCTTGCTAACCGTGCCCGCAGTAAGCAAATTAAGCTTCCTGATTTAAGTAAGGGAACGATCTCGGTAACTAACTTTGGTTCTTTAGGCGCTGCTTTTGGTACCCCGATTATTAAGCACCCGGAAATGTGTATTGTCGCTACCGGTAACATGGAAGAACGTGTTGTGCGTGCTGAAGGTGGGGTAGCCGTTCATACCATCCTACCGTTAACAATTGCTGCCGACCACCGCTGGGTCGATGGCGCTGATGTGGGTCGCTTTGGTAAGGAAATTGCCAAGCAAATCGAAGAATTAATTGATTTAGAAGTAGCTTAG", "aa": "MANEFKFTDVGEGLHEGKVTEILKKVGDTIKVDEALFVVETDKVTTELPSPYAGVITAITTNVGDVVHIGQVMAVIDDGAGAAAPAAPQPVSAPAPAPTPTFTPTPAPVTTEPVVEEAGASVVGEIKVSNSVFPIFGVQPSAPQPTPAPVVQPTSAPTPTPAPASAAAPSGEETIAITTMRKAIAEAMVKSHENIPATILTFYVNATKLKQYRESVNGLALSKYNMKISFFAFFVKAIVNALKKFPVFNGRYDKERNLIVLNKDVNVGIAVDTPDGLIVPNIKQAQTKSVVDIAKDIVDLANRARSKQIKLPDLSKGTISVTNFGSLGAAFGTPIIKHPEMCIVATGNMEERVVRAEGGVAVHTILPLTIAADHRWVDGADVGRFGKEIAKQIEELIDLEVA", "aa_hexdigest": "eb234ec07be3b945730db8634bb71aab", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000130BFE", "ncbi_nrp_id": "WP_010874747.1", "uniref100_id": "UniRef100_P75392", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000130BFE", "RefSeq:WP_010874747.1", "UniRef:UniRef100_P75392" ] }, "ips": { "uniref100_id": "UniRef100_P75392", "uniref90_id": "UniRef90_P75392" }, "psc": { "uniref90_id": "UniRef90_P75392", "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "ec_ids": [ "2.3.1.12" ], "uniref50_id": "UniRef50_P75392", "cog_id": "COG0508", "cog_category": "C", "go_ids": [ "GO:0009986", "GO:0005829", "GO:0016020", "GO:0004742", "GO:0031405" ] }, "pscc": { "uniref50_id": "UniRef50_P75392", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75392" ], "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex" }, "genes": [ "pdhC" ], "id": "CNOCLNGIAL_847", "locus": "GCA000143945_00826" }, { "type": "cds", "sequence": "contig_1", "start": 467518, "stop": 467754, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02780.25" ], "nt": "GTGTTTAACTCCGTGAAGAAAACCGGGCGCTTGCTCGTAGTTACCGAAGCGGCTAAAACCTTTACCACCAGTGGGGAAATTATTGCATCGGTAACTGAAGAATTATTTAGCTATCTCAAGGCAGCCCCACAACGGGTAACCGGTTGGGACATTGTGGTACCGTTAGCACGGGGTGAACACTACCAGTTCAATTTAAATGCCCGCATTTTGGAAGCTGTTAACCAACTTTTAAAGTAA", "aa": "MFNSVKKTGRLLVVTEAAKTFTTSGEIIASVTEELFSYLKAAPQRVTGWDIVVPLARGEHYQFNLNARILEAVNQLLK", "aa_hexdigest": "42e4b34466ce553931a8307c3bb2b9f6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02780.25", "name": "Transketolase_C", "length": 60, "aa_cov": 0.7692307692307693, "hmm_cov": 1.0, "evalue": 1.5167410675103731e-13, "score": 46.22168731689453, "start": 3, "stop": 62 } ], "seq_stats": { "molecular_weight": 8694.967899999996, "isoelectric_point": 9.221831703186034 }, "id": "CNOCLNGIAL_848", "locus": "GCA000143945_00827" }, { "type": "cds", "sequence": "contig_1", "start": 467767, "stop": 468501, "strand": "-", "frame": 3, "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "db_xrefs": [ "COG:C", "COG:COG0022", "EC:1.2.4.1", "GO:0001968", "GO:0004739", "GO:0005829", "GO:0009897", "GO:0009986", "GO:0016020", "GO:0031639", "GO:0033111", "GO:0051919", "SO:0001217", "UniRef:UniRef50_P75391", "UniRef:UniRef90_P75391" ], "nt": "ATGTCAAAAACAATTCAAGCAAATAACATTGAAGCGTTGGGTAACGCAATGGATCTAGCGTTAGAGCGCGACCCCAACGTTGTTTTATACGGTCAGGATGCTGGCTTTGAAGGTGGTGTGTTCCGTGCTACTAAGGGTTTACAAAAGAAGTACGGTGAGGAGCGTGTTTGGGACTGTCCGATTGCCGAAGCAGCTATGGCTGGTATTGGTGTGGGCGCTGCCATTGGTGGACTTAAACCAATCGTGGAAATCCAGTTCTCCGGTTTTTCTTTCCCCGCAATGTTCCAAATTTTTACCCACGCAGCACGGATCCGCAACCGTTCTCGCGGTGTGTATACCTGTCCGATTATTGTCCGCATGCCAATGGGTGGTGGGATTAAAGCACTTGAACACCACAGTGAGACCTTGGAAGCGATTTACGGTCAAATTGCCGGACTTAAAACAGTGATGCCGTCCAACCCTTACGACACCAAGGGCTTATTCTTAGCAGCAGTGGAATCACCTGACCCTGTGGTCTTCTTTGAACCAAAGAAGCTGTACCGTGCCTTCCGTCAGGAAATTCCAGCAGATTATTACACTGTGCCAATTGGTCAAGCTAACTTAATTAGCCAGGGTAACAACCTTACTATTGTTAGTTACGGTCCTACCATGTTTGACCTAATTAACATGGTCTATGGGGGTGAGTTGAAGGACAAGGGCATTGAACTCATTGACTTGCGTACCATTTCCCCTTGA", "aa": "MSKTIQANNIEALGNAMDLALERDPNVVLYGQDAGFEGGVFRATKGLQKKYGEERVWDCPIAEAAMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFTHAARIRNRSRGVYTCPIIVRMPMGGGIKALEHHSETLEAIYGQIAGLKTVMPSNPYDTKGLFLAAVESPDPVVFFEPKKLYRAFRQEIPADYYTVPIGQANLISQGNNLTIVSYGPTMFDLINMVYGGELKDKGIELIDLRTISP", "aa_hexdigest": "60542a124bde51fe8466edcf9360fd6d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FDA3C09", "query_cov": 0.930327868852459, "subject_cov": 0.9700854700854701, "identity": 0.555, "score": 267.0, "evalue": 3.08e-87, "valid": false, "product": "transketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A537VMS1" }, "genes": [ "pdhB" ], "truncated": "5-prime", "pseudogene": { "start": 467518, "stop": 468501, "observations": { "mutation": [ 467769 ], "stop": [ 467769 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75391", "query_cov": 1.0, "subject_cov": 0.746177370030581, "identity": 1.0, "score": 490.0, "evalue": 4.45e-174, "gene_start": 1, "gene_end": 244, "reference_start": 1, "reference_end": 244, "reference_sequence": "MSKTIQANNIEALGNAMDLALERDPNVVLYGQDAGFEGGVFRATKGLQKKYGEERVWDCPIAEAAMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFTHAARIRNRSRGVYTCPIIVRMPMGGGIKALEHHSETLEAIYGQIAGLKTVMPSNPYDTKGLFLAAVESPDPVVFFEPKKLYRAFRQEIPADYYTVPIGQANLISQGNNLTIVSYGPTMFDLINMVYGGELKDKGIELIDLRTISPWDKETVFNSVKKTGRLLVVTEAAKTFTTSGEIIASVTEELFSYLKAAPQRVTGWDIVVPLARGEHYQFNLNARILEAVNQLLK" }, "paralog": false, "identity": 0.9969418960244648, "score": 646.0, "evalue": 9.09e-238, "description": "Internal stop codon at 467769. Nonsense mutation around 467769.", "psc": { "uniref90_id": "UniRef90_P75391", "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "ec_ids": [ "1.2.4.1" ], "uniref50_id": "UniRef50_P75391", "cog_id": "COG0022", "cog_category": "C", "go_ids": [ "GO:0033111", "GO:0009986", "GO:0005829", "GO:0009897", "GO:0016020", "GO:0001968", "GO:0004739", "GO:0031639", "GO:0051919" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75391", "EC:1.2.4.1", "UniRef:UniRef50_P75391", "COG:COG0022", "COG:C", "GO:0033111", "GO:0009986", "GO:0005829", "GO:0009897", "GO:0016020", "GO:0001968", "GO:0004739", "GO:0031639", "GO:0051919" ] }, "pscc": { "uniref50_id": "UniRef50_P75391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75391" ], "product": "Pyruvate dehydrogenase E1 component subunit beta" } }, "id": "CNOCLNGIAL_849", "locus": "GCA000143945_00828" }, { "type": "cds", "sequence": "contig_1", "start": 468523, "stop": 468759, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAGTGACCCTGTGAAGCGGTTACGTAACTTCTTGTTTGACCGTGGTATTCTAACCCCACAACAAGAAGAGGAAATGGTAGCTAAGATTGAACAAGAAGTGCAAGCTGCATATGAGGTAATGGTTAGCAAAACGCCAGTTACCTTGGATGAAGTGTTTGATTACAACTATGAAAAGTTGACTCCTGATCTAGCGCGCCAAAAGGCTGAAGCTAAAAAATACTTTAAAGACTAA", "aa": "MKSDPVKRLRNFLFDRGILTPQQEEEMVAKIEQEVQAAYEVMVSKTPVTLDEVFDYNYEKLTPDLARQKAEAKKYFKD", "aa_hexdigest": "22a018bb5e0a188eee3497f3ddf07ced", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9202.413899999992, "isoelectric_point": 5.180442237854003 }, "id": "CNOCLNGIAL_850", "locus": "GCA000143945_00829" }, { "type": "cds", "sequence": "contig_1", "start": 469048, "stop": 469158, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTGCACTACAAGAAACTCCCTAACGTAGCCGTTACGATGATTGGGGATGGTGGTACAGCTGAAGGTGAGTTTTATGAAGCGATGAACATTGCTTCCATTCACAAGTGA", "aa": "MLHYKKLPNVAVTMIGDGGTAEGEFYEAMNIASIHK", "aa_hexdigest": "cd628000bc1b3f624d4cf663fe100233", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 34, "aa_cov": 0.9444444444444444, "hmm_cov": 1.0, "evalue": 1.873500019883961e-09, "score": 32.36134338378906, "start": 3, "stop": 36 } ], "seq_stats": { "molecular_weight": 3937.5207, "isoelectric_point": 5.9887495040893555 }, "id": "CNOCLNGIAL_851", "locus": "GCA000143945_00830" }, { "type": "cds", "sequence": "contig_1", "start": 469261, "stop": 469413, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTGAACTTTGCGCCTAACTTAGGTGAGGAAGCACTGCAAGTTGGTATGGGCATGGGTTTAAATGAAAATGACTGGTTCTGCCCTACCTTCCGTTCCGGTGCGTTAATGTTGTACCGTGGTGTTAAACCAGAACAGCTTTTACTTTACTGA", "aa": "MLNFAPNLGEEALQVGMGMGLNENDWFCPTFRSGALMLYRGVKPEQLLLY", "aa_hexdigest": "fdbb75e2166b67bf059591c6d23abef4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 45, "aa_cov": 0.9, "hmm_cov": 1.0, "evalue": 7.38276180909935e-08, "score": 27.122459411621094, "start": 5, "stop": 49 } ], "seq_stats": { "molecular_weight": 5636.5244999999995, "isoelectric_point": 4.584314918518066 }, "id": "CNOCLNGIAL_852", "locus": "GCA000143945_00831" }, { "type": "cds", "sequence": "contig_1", "start": 469429, "stop": 469599, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTTTGATTAAAAACAAAGTCCCTACTACACTTTACCAAGTGTATGACAATGAGGGTAAGTTAATGGACCCTAATCACAAGATTACACTTAGCAACGAACAGTTAAAGCACGCTTTTTACCTCATGAATCTTTCCCGAATCATGGACAAGAAGATGCTTGTTTGA", "aa": "MAILIKNKVPTTLYQVYDNEGKLMDPNHKITLSNEQLKHAFYLMNLSRIMDKKMLV", "aa_hexdigest": "5bab2ac35b6accac1ac55ef5124ba67c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6594.8087, "isoelectric_point": 9.398539543151855 }, "id": "CNOCLNGIAL_853", "locus": "GCA000143945_00832" }, { "type": "cds", "sequence": "contig_1", "start": 469620, "stop": 469748, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGATCAGTGAACTGGGTTTAGTAGACGTTTACTTTCTTCCCCACTACAATAAGCCGTTCAACTTTGTGTTAGCAGCCGTGTTGCAAGCGCTTGGTTTTAGTTACTATACCCCTAAAAATAAATAA", "aa": "MLISELGLVDVYFLPHYNKPFNFVLAAVLQALGFSYYTPKNK", "aa_hexdigest": "445622aaa8dfa37c456841e475e603d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4822.619100000001, "isoelectric_point": 8.085060691833494 }, "id": "CNOCLNGIAL_854", "locus": "GCA000143945_00833" }, { "type": "cds", "sequence": "contig_1", "start": 469803, "stop": 470468, "strand": "-", "frame": 1, "gene": "nox", "product": "NADH oxidase", "db_xrefs": [ "COG:COG0446", "COG:I", "EC:1.6.3.4", "GO:0008137", "SO:0001217", "UniRef:UniRef50_Q49408", "UniRef:UniRef90_P75389" ], "nt": "ATGTTGGACAAACCAGCTGGCAATAACTTTGATGAAGAGTTTACCAACGAGTTGGAAAAAGCCATGAAGAAAGCGGGCATTAACCTAATGATGGGTAGTGCCGTTAAGGGCTTTATTGTTGATGCGGACAAAAATGTCGTTAAAGGTGTTGAAACCGATAAGGGTCGGGTGGATGCTGACCTTGTCATCCAATCGATTGGGTTCCGCCCAAACACCCAGTTTGTCCCTAAAGACAGGCAGTTTGAGTTTAACCGTAACGGTTCGATTAAGGTTAATGAATACCTCCAAGCCTTAAATCATGAAAATGTGTATGTTATTGGTGGGGCTGCTGCCATTTATGATGCAGCTAGTGAACAATACGAAAACATTGACTTAGCTACCAACGCTGTGAAAAGTGGTTTAGTCGCTGCTATGCACATGATTGGTAGCAAGGCTGTAAAGTTAGAGTCCATTGTTGGTACCAATGCGCTGCACGTATTTGGTTTAAATCTAGCAGCTACTGGTTTAACCGAAAAACGTGCCAAAATGAACGGTTTTGATGTGGGAGTGAGCATTGTTGATGACAACGACCGTCCCGAATTTATGGGGACCTTTGACAAGGTGCGCTTTAAGTTAATTTACGACAAGAAGACGCTCCGTTTGTTGGGTGCCCAATTACTTTCGTGA", "aa": "MLDKPAGNNFDEEFTNELEKAMKKAGINLMMGSAVKGFIVDADKNVVKGVETDKGRVDADLVIQSIGFRPNTQFVPKDRQFEFNRNGSIKVNEYLQALNHENVYVIGGAAAIYDAASEQYENIDLATNAVKSGLVAAMHMIGSKAVKLESIVGTNALHVFGLNLAATGLTEKRAKMNGFDVGVSIVDDNDRPEFMGTFDKVRFKLIYDKKTLRLLGAQLLS", "aa_hexdigest": "3f23ecc26d2f5074f2ab9cc1410e1184", "start_type": "ATG", "rbs_motif": null, "genes": [ "nox" ], "truncated": "both", "pseudogene": { "start": 469620, "stop": 470768, "observations": { "mutation": [ 469805, 470501, 470717 ], "stop": [ 469805, 470501, 470717 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75389", "query_cov": 1.0, "subject_cov": 0.4613778705636743, "identity": 1.0, "score": 429.0, "evalue": 7.4e-148, "gene_start": 1, "gene_end": 221, "reference_start": 198, "reference_end": 418, "reference_sequence": "MKKVIVIGVNHAGTSFIRTLLSKSKDFQVNAYDRNTNISFLGCGIALAVSGVVKNTEDLFYSTPEELKAMGANVFMAHDVVGLDLDKKQVIVKDLATGKETVDHYDQLVVASGAWPICMNVENEVTHTQLQFNHTDKYCGNIKNLISCKLYQHALTLIDSFRHDKSIKSVAIVGSGYIGLELAEAAWQCGKQVTVIDMLDKPAGNNFDEEFTNELEKAMKKAGINLMMGSAVKGFIVDADKNVVKGVETDKGRVDADLVIQSIGFRPNTQFVPKDRQFEFNRNGSIKVNEYLQALNHENVYVIGGAAAIYDAASEQYENIDLATNAVKSGLVAAMHMIGSKAVKLESIVGTNALHVFGLNLAATGLTEKRAKMNGFDVGVSIVDDNDRPEFMGTFDKVRFKLIYDKKTLRLLGAQLLSWNTNHSEIIFYIALAVQKKMLISELGLVDVYFLPHYNKPFNFVLAAVLQALGFSYYTPKNK" }, "paralog": false, "identity": 0.9921465968586387, "score": 746.0, "evalue": 3.84e-275, "description": "Internal stop codon at 469805, 470501, 470717. Nonsense mutation around 469805, 470501, 470717.", "psc": { "uniref90_id": "UniRef90_P75389", "gene": "nox", "product": "NADH oxidase", "ec_ids": [ "1.6.3.4" ], "uniref50_id": "UniRef50_Q49408", "cog_id": "COG0446", "cog_category": "I", "go_ids": [ "GO:0008137" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75389", "EC:1.6.3.4", "UniRef:UniRef50_Q49408", "COG:COG0446", "COG:I", "GO:0008137" ] }, "pscc": { "uniref50_id": "UniRef50_Q49408", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49408" ], "product": "NADH oxidase" } }, "id": "CNOCLNGIAL_855", "locus": "GCA000143945_00834" }, { "type": "cds", "sequence": "contig_1", "start": 470499, "stop": 470705, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTGGAAAACGAAGTAACCCACACCCAATTGCAATTCAACCACACTGATAAGTACTGTGGCAATATTAAGAACTTAATTAGTTGTAAGTTGTACCAACACGCCCTTACCTTAATTGACAGCTTCCGTCATGACAAGTCAATTAAATCAGTAGCCATTGTGGGCTCGGGTTACATTGGTTTAGAGTTAGCAGAAGCGGCTTGA", "aa": "MNVENEVTHTQLQFNHTDKYCGNIKNLISCKLYQHALTLIDSFRHDKSIKSVAIVGSGYIGLELAEAA", "aa_hexdigest": "2dc24f838a546b751a95cb4b1fe21983", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7592.557099999999, "isoelectric_point": 6.380313301086425 }, "id": "CNOCLNGIAL_856", "locus": "GCA000143945_00835" }, { "type": "cds", "sequence": "contig_1", "start": 470715, "stop": 471059, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07992.20" ], "nt": "ATGAAGAAAGTGATTGTTATCGGTGTCAATCACGCCGGCACCAGTTTTATCAGAACCCTTTTAAGCAAGAGCAAAGACTTTCAGGTCAACGCTTATGATCGCAATACCAACATCTCCTTTTTGGGTTGTGGAATTGCCTTGGCAGTTAGTGGTGTAGTTAAAAACACCGAAGACCTGTTCTACTCCACCCCTGAAGAGCTAAAAGCTATGGGTGCTAATGTCTTTATGGCTCATGATGTAGTTGGTCTTGATCTAGACAAAAAACAAGTAATCGTGAAGGACTTGGCCACTGGTAAGGAAACTGTGGACCACTATGATCAATTGGTAGTAGCCTCTGGAGCTTGA", "aa": "MKKVIVIGVNHAGTSFIRTLLSKSKDFQVNAYDRNTNISFLGCGIALAVSGVVKNTEDLFYSTPEELKAMGANVFMAHDVVGLDLDKKQVIVKDLATGKETVDHYDQLVVASGA", "aa_hexdigest": "5b3d93c88d0a16c4b7e8d15b26fa8f75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 115, "aa_cov": 0.9912280701754386, "hmm_cov": 0.9478260869565217, "evalue": 2.9569830997556263e-16, "score": 55.137691497802734, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 12303.003799999991, "isoelectric_point": 6.256517982482911 }, "id": "CNOCLNGIAL_857", "locus": "GCA000143945_00836" }, { "type": "cds", "sequence": "contig_1", "start": 471127, "stop": 471660, "strand": "-", "frame": 3, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_014574965.1", "SO:0001217", "UniParc:UPI0001DD63CF", "UniRef:UniRef100_A0A0H3DNR1", "UniRef:UniRef50_P75388", "UniRef:UniRef90_P75388" ], "nt": "GTGAAACAGAAACTCCAAGCGCTTGATCGAGCTATTAAGCGATTTAATGACTTTCCCACTCCGGGAATCCTCTTTTACGACATTACGCCAATTTTTTTAAACAGTGAACTGTTTGAGTTTGTACTGGAACAAATGGCACAGTTTATCCAGGAAGTTAAAGCTGATGGCATCGTATGTCCCGAAGCGCGTGGTTTTATCTTTGGTGGTGCTTTGGCTTCCAAAACCAAACTACCCCTAGTGTTAGTGCGCAAACCCCATAAGCTATCAGGTGAGTTAGCGCGTGAAACCTATGATTTGGAATACCGCCAAAACTCAATCTTGGAAATGCGGGTTGATGCACTAGAAAACTGCAAGCGCTGTGTGATTGTTGATGACTTACTAGCTACTGCTGGTACGGTTGCAGCGATTGACAAACTAATAGCGAGTTTAGGTAGTCAAACTGTGGGTTATTGTTTTTTAATTGAACTGCAAAAACTGCACGGTAAAGCAAAGCTACAACCAAATGTGGCAACCAAGATCTTGCTCCACTATTAA", "aa": "MKQKLQALDRAIKRFNDFPTPGILFYDITPIFLNSELFEFVLEQMAQFIQEVKADGIVCPEARGFIFGGALASKTKLPLVLVRKPHKLSGELARETYDLEYRQNSILEMRVDALENCKRCVIVDDLLATAGTVAAIDKLIASLGSQTVGYCFLIELQKLHGKAKLQPNVATKILLHY", "aa_hexdigest": "16ecc9f13d930c8d27c27c68929615bb", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63CF", "ncbi_nrp_id": "WP_014574965.1", "uniref100_id": "UniRef100_A0A0H3DNR1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63CF", "RefSeq:WP_014574965.1", "UniRef:UniRef100_A0A0H3DNR1" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DNR1", "uniref90_id": "UniRef90_P75388" }, "psc": { "uniref90_id": "UniRef90_P75388", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_P75388", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_P75388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75388" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "CNOCLNGIAL_858", "locus": "GCA000143945_00837" }, { "type": "cds", "sequence": "contig_1", "start": 471695, "stop": 471946, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTACGCTGCCTTTAACTTTGGTGGTATTAACGGCACGATTAACCTAGTGATCTTCTACTTAATTGCGATTGTGGCCTTGTTTAGCGTGTTTGTCAATGGCTTACCGCTGTTTAGCTTTGGTTTAATCAATGGTTGGTTAAGTCCGTACAACTATGTCCAAATCCACATTACCTTACGCCACAAAAAGCAAATGTTTAAGGAAGTGGATCAAATTGAAGAACAGTTGATTAGTGGAATTAACAGCTTTTAA", "aa": "MYAAFNFGGINGTINLVIFYLIAIVALFSVFVNGLPLFSFGLINGWLSPYNYVQIHITLRHKKQMFKEVDQIEEQLISGINSF", "aa_hexdigest": "fc43ab3fc82cab266e16351a1a6b4022", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9445.953199999996, "isoelectric_point": 6.702078819274903 }, "id": "CNOCLNGIAL_859", "locus": "GCA000143945_00838" }, { "type": "cds", "sequence": "contig_1", "start": 471992, "stop": 472543, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCAAAACATCGAGTAACTGCTCGATCTTAACGGAAAACAACGACATCCAAACGGACTTCTTTATCAGTAAGAACCAGCACGACTTCTTTGGTAAGAAAAAGTGGAGCTTGCTGTTTATTTGGTTGACAGTTCTCGCCTTTGGGGTGGTGATGCTCGGCCTTTATCTGGGCGCACCACAACTGTTAGGTAACTTTTTAGCCGCTGACATCGAATCGAGTACTGGCATTATGTTAGGTGTGGGGGTGATTAGCTTGCTCTATCTCTTGTATTCGCTACCGCGCTATGGTGTCGCTTATGGGATTAGCTACCTAATTGCTTTAATTCTGTTAGCCGCTGGACTTTTTGGGGCAATGTACCTCGCTAACTTTATCTTTAGGATTGACCAGTCCACAATTCAACTAATTATCTTTGTGTACCTGTTCTGGCTGTTCTTCCAAGCCCGCATTGGTCAAACTACCATTTGGACTTACTGTTACTGGTTTAAACGCAGCTTGAAGGACCGGGTCTTTGTGAAAAACCTCTTTAACGACAATGTTAACAGCCAATGA", "aa": "MTKTSSNCSILTENNDIQTDFFISKNQHDFFGKKKWSLLFIWLTVLAFGVVMLGLYLGAPQLLGNFLAADIESSTGIMLGVGVISLLYLLYSLPRYGVAYGISYLIALILLAAGLFGAMYLANFIFRIDQSTIQLIIFVYLFWLFFQARIGQTTIWTYCYWFKRSLKDRVFVKNLFNDNVNSQ", "aa_hexdigest": "26f9b328aae16640eddba6d161144e39", "start_type": "TTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20931.366900000005, "isoelectric_point": 9.076004219055175 }, "id": "CNOCLNGIAL_860", "locus": "GCA000143945_00839" }, { "type": "cds", "sequence": "contig_1", "start": 472577, "stop": 472759, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGAAACCCACATTTGCTGGTTATTGAGTGCCTTAGTCGTGATTTACCTCTCCAACTACCAGGTACAACAGTTAGGTACCTTAATGGCAGTTTCAGCGATTACCTCTTACTTCTTAAACTATGGTTTAGGAGTAATCTTAGTGTGGTTGTTTGTGTCATCCCATTCCGGGTTAGAATGA", "aa": "MIETHICWLLSALVVIYLSNYQVQQLGTLMAVSAITSYFLNYGLGVILVWLFVSSHSGLE", "aa_hexdigest": "ac302e42dfe3dbde8c9e7e7eca00c1a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6706.840099999998, "isoelectric_point": 5.364145851135253 }, "id": "CNOCLNGIAL_861", "locus": "GCA000143945_00840" }, { "type": "cds", "sequence": "contig_1", "start": 472790, "stop": 473719, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF44FC6", "UniRef:UniRef90_UPI000AF44FC6" ], "nt": "GTGCGCAACATCTTTAACGTTCCTAAAGGTGGTACTGATTACCTCACCTTTAACGAACGTGAAAAGAACCTCTGGGACTTCTTGCACGCTGAGGGTAACTTTTCCTCTGGTGATAATTTGTTTTTGCAAAAAAATAATAATGGTAATTCACCAATTACCAAAGCTAGTGACATTACCTTAAAAAATATCTATTACATCTATGCAGCACCGACACATTTCTCACAAGTTGCAACCAAAGACAATAACAATAAGGACGCTGCTGATGTTGTCACTGTAGCTAACAGCGCGGACACTAGAAAACTGCGCAGCGGTGATGCTACTGGTTTTTCTGGTTTATTCTCCAATTACATCTTGGCAGAAATTAGAACAGAAGATCCGGTAGCTAAGAATGGTGATCCGGTTAAGGTAAACGCCACTTTACAAAGCTTTTTGGATAGCAATAACCAACGCTTAGAACACGGTGGTAACATTAAGTTCCAAGTAGCTAACTTTGTTAACAATGATGGTTCATACGTTCCTGCTTCTTACTTGGAAGCTAGTAGGGTAAAGGTGCTGTTGAGCAATGGTTTTCCCGAAACCGCTACCATTGCAGCTGCTAACACCAAGTTAGTTAATGCACCGCTTTCCAACACTTTAGCACGCGATGTTTCTAACTTTGCTTCGAGCTTCATTGCCTTGGGTGTAATTATCCTGCTCGCTACAATTGCGTTAGGTATTCGTTACAAGCTACTTGGCCTTTACAAAGCCTTAGCCTTAGCGCTTAGTGCCATCTCTTCGCTCGCCTTCTTTAATGCCGTTGGTGGTGTCGTTGATGTCTTTTCGTTTGTCGGCATCTTCTTTGTGGTTGCCGTTAACATTATTAACCTGATCACTCTGGCGGAACTGTTTTTGCGTAACATTAAGAACAACGCCTCAATTGTGGAATCGTGA", "aa": "MRNIFNVPKGGTDYLTFNEREKNLWDFLHAEGNFSSGDNLFLQKNNNGNSPITKASDITLKNIYYIYAAPTHFSQVATKDNNNKDAADVVTVANSADTRKLRSGDATGFSGLFSNYILAEIRTEDPVAKNGDPVKVNATLQSFLDSNNQRLEHGGNIKFQVANFVNNDGSYVPASYLEASRVKVLLSNGFPETATIAAANTKLVNAPLSNTLARDVSNFASSFIALGVIILLATIALGIRYKLLGLYKALALALSAISSLAFFNAVGGVVDVFSFVGIFFVVAVNIINLITLAELFLRNIKNNASIVES", "aa_hexdigest": "a7530daa11969610a7f7d29b90b59a08", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 472487, "stop": 473890, "observations": { "mutation": [ 472579, 472792, 473806 ], "stop": [ 472579, 472792, 473806 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF44FC6", "query_cov": 1.0, "subject_cov": 0.42270861833105333, "identity": 0.997, "score": 576.0, "evalue": 1.17e-200, "gene_start": 1, "gene_end": 309, "reference_start": 58, "reference_end": 366, "reference_sequence": "MDFIKSKSLTDSDYNTGNGQKSVEGSGKWFKDKANGSAQNGNKTLVLWKDKEGAVQYVRNIFNVPKGGTDYLTFNEREKNLWDFLHAEGNFSSGDNLFLQKNNNGNSPITKASDITLKNIYYIYAAPTHFSQVATKDNNNKDAADVVTVANSADTRKLRSGDATGFSGLFSNYILAEIRTEDPVAKNGDPVKVNATLQSFLDSNNQRLEHGGNIKFQVANFVNNDGSYVPASYLEASRVKVLLSNGFPETATIAAANTKLVNAPLSNTLARDVSNFASSFIALGVIILLATIALGIRYKLLGLYKALALALSAISSLAFFNAVGGVVDVFSFVGIFFVVAVNIINLITLAELFLRNIKNNASIVESWKLCLKRSFFTMIETHICWLLSALVVIYLSNYQVQQLGTLMAVSAITSYFLNYGLGVILVWLFVSSHSGLEWRFFLYKKDAQALTKTSSNYSILTENNDIQTDFFISKNQHDFFGKKKWSLLFIWLTVLAFGVVMLGLYLGAPQLLGNFLAADIESSTGIMLGVGVISLLYLLYSLPRYGVAYGISYLIALILLAAGLFGAMYLANFIFRIDQSTIQLIIFVYLFWLFFQARIGQTTIWTYCYWFKRSLKDRVFVKNLFNDNVNSQWRLDLIGSSVLILLFIVYAAFNFGGINGTINLVIFYLIAIVALFSVFVNGLPLFSFGLINGWLSPYNYVQIHITLRHKKQMFKEVDQIEEQLISGINSF" }, "paralog": false, "identity": 0.9892933618843683, "score": 897.0, "evalue": 0.0, "description": "Internal stop codon at 472579, 472792, 473806. Nonsense mutation around 472579, 472792, 473806.", "psc": { "uniref90_id": "UniRef90_UPI000AF44FC6", "uniref50_id": "UniRef50_UPI000AF44FC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF44FC6", "UniRef:UniRef50_UPI000AF44FC6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF44FC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF44FC6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 33403.45650000003, "isoelectric_point": 7.841628074645997 }, "id": "CNOCLNGIAL_862", "locus": "GCA000143945_00841" }, { "type": "cds", "sequence": "contig_1", "start": 474203, "stop": 474610, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACTGAAAAAGCGGGTTTCCTTTGACTGGCTCTTAAAGATTGGAATTATCCTGGTCTTAGCTTTTTTGGGCCTGTTTGGAATTATTTTTGGTAGTTATAAGCTTCTAAACGACAGTCGCTTAGGGGTAGTGTTCAACGGTTCCACTACCACCACTGTTTATTTTTTAAACCATAAGTCCAAGGACGCTAACAAGGAGTTAGATCCTACCCAGAAAAAGGACGGCAATGGCAACGGTAACAATACTGAAATGATTACCGATGTGAACGGTTTTCTCAACAATATTGAAAAGAGTTACGCCAACAGCCTCTACGCCCAGGGTTTTTCGTCTGTTAACATCACGAAAAACTCACACGATACAAGTGCAAAAGAACTTTCAGATAAAGGAGTTTTTGACAGTTCTTGA", "aa": "MGLKKRVSFDWLLKIGIILVLAFLGLFGIIFGSYKLLNDSRLGVVFNGSTTTTVYFLNHKSKDANKELDPTQKKDGNGNGNNTEMITDVNGFLNNIEKSYANSLYAQGFSSVNITKNSHDTSAKELSDKGVFDSS", "aa_hexdigest": "7b13d6e5c114d116c04d20bbdbc27d57", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14821.56319999998, "isoelectric_point": 8.803883171081544 }, "id": "CNOCLNGIAL_863", "locus": "GCA000143945_00842" }, { "type": "cds", "sequence": "contig_1", "start": 474653, "stop": 474763, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCACTTTAGTTGAAATTGAACGCGATTTTTTACAAAAAACAGCCCAAAAATTTGCACCTGAAGTGGTTGCGTTGATTACTAAAGCACTCGATTACTCCAAAAAGTGA", "aa": "MATLVEIERDFLQKTAQKFAPEVVALITKALDYSKK", "aa_hexdigest": "ddd94cdd00635392bc3c5ea8e08e66f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4096.786800000001, "isoelectric_point": 8.099243736267091 }, "id": "CNOCLNGIAL_864", "locus": "GCA000143945_00843" }, { "type": "cds", "sequence": "contig_1", "start": 474845, "stop": 476308, "strand": "+", "frame": 2, "gene": "spoT", "product": "putative guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "db_xrefs": [ "COG:COG0317", "COG:TK", "EC:3.1.7.2", "GO:0005886", "GO:0008893", "GO:0015970", "SO:0001217", "UniRef:UniRef50_P75386", "UniRef:UniRef90_P75386" ], "nt": "ATGGACAGCAACACCGTGTGTGCTGGTCTGTTACACGACATTATTGAAGATACCCAGGTCACTGAAGCTGATTTAACTGCCATTTTTGGTAAAGAAATTACTGATTTAGTGGTAAAAGTTACCAAAATTACCAGTGAATCCAAAAAACAACGCCAGCTAAACCGAAAGAAAGAAGATTTAAACCTCAAAAGTTTGGTTAACATTGCGATGAGCTCGCAGCAAGAGGTCAATGCTTTGGTGTTAAAGTTGGCCGACCGACTCGACAACATCTCCTCAATTGAATTTTTAGCAGTCGAAAAACAAAAGATTATTGCTAAAGAAACACTCGAACTGTATGCCAAGATAGCCGGTCGAATTGGAATGTATCCCGTTAAAACTCAACTAGCCGATCTTTCCTTTAAGGTATTAGATCCAAAAAACTTTAACAATACGCTGTCCAAGATTAACCAGCAAAAGGTCTTTTATGACAACGAATGGGACAACTTTAAAAAACAGTTGGAAGAGATGCTGGAACAAAACCAGATTGAATACCGCTTAGAAAGTCGAATTAAAGGCATTTATTCTACTTATCAAAAATTAACCTTTCACGAGCAGAACATTGCCAAAATCCACGATCTGTTCGCCATTCGTTTAATTGTGAAGTCAGAGTTGGATTGTTACCACTTACTTGGTTTAATCCACCTCAACTTTACCGTGTTAATGAAACACTTTAAGGATTACATAGCATCCCCCAAACAAAACTTTTACCAATCAATCCACACGACTGTGCGCTTGAAGGGTTTAAATGTGGAAATCCAGATTAGAACCCAACGAATGGATCATGTGAGCAAGTACGGTTTTGCTAGCCACTGGATTTACAAAGAGAAAAAGGAAGGGTTATTGGCAAGTGCTTTACAAGTAAATTACTTAAACAGTAAACAAATGCATTCGCGCGATTTTTTTAAACGAATCTTTGGTACTGATATTATTAAGGTTAATGTAAGTTCCGATAACGAACCTAACATAGTCAAAAAACTCAATGTTGAAAGTAACAGCAAGTTGCTCGATATTGCTTATGAGTTGTATCCCAAACAGTTCAATAAATTAGAAAAAATCAAACTCGATGGGGTTGAGGTTATGTCGTTTGACGTAACTGCCGAAAACGAAATGGTAATTGAGTTTTGTTTTGGTAAAACCAATAACCTCAAACGGCGTTGGTTACGTTATATGAATAACCCCGTATTTCGCGAACGGGTTAAAAAGGATTTAAACAAGTTAAAGAAAGCGGTTAAGTACAGTGAACTACCTCTATACGAAAAGGCCTTGGAAGAGTTGCACTTAAAGCTAGCTGATGAAACCCAGATTAAACAACGCTTAAATGCTTTAGGGATCAAAAAACTGACAGAGTTCTTGGAATTAATCGAATACCCCCACTTCCCTAAAAATGAGCATTTGTACTTTTTAGCTAGTAACAATCAGAAGTGA", "aa": "MDSNTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSESKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKLADRLDNISSIEFLAVEKQKIIAKETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWDNFKKQLEEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLIHLNFTVLMKHFKDYIASPKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIYKEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDIIKVNVSSDNEPNIVKKLNVESNSKLLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYMNNPVFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLELIEYPHFPKNEHLYFLASNNQK", "aa_hexdigest": "7d78d64061dc10d15ea814f4569315b2", "start_type": "ATG", "rbs_motif": null, "genes": [ "spoT" ], "truncated": "both", "pseudogene": { "start": 474614, "stop": 476611, "observations": { "mutation": [ 474626, 474761, 474839, 476306, 476546 ], "stop": [ 474626, 474761, 474839, 476306, 476546 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75386", "query_cov": 1.0, "subject_cov": 0.6643929058663028, "identity": 0.996, "score": 933.0, "evalue": 0.0, "gene_start": 1, "gene_end": 487, "reference_start": 78, "reference_end": 564, "reference_sequence": "MFYNWLKLYKFSKMATLVEIERDFLQKTAQKFAPEVVALITKALDYSKKWHGEQKRLSGEPFFIHPLRTALRLVEWNMDSNTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSESKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKLADRLDNISSIEFLAVEKQKIIAKETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWGNFKKQLEEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLIHLNFTVLMKHFKDYIASPKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIYKEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDIIKVNVSSDNEPNIVKKLNVESNSKLLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYMNNHVFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLELIEYPHFPKNEHLYFLASNNQKWRELIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCTKIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKIIPLYWNAHQLKMRPRKFRCQINIRGVWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQITTKNISYVWKYL" }, "paralog": false, "identity": 0.9894736842105263, "score": 1289.0, "evalue": 0.0, "description": "Internal stop codon at 474626, 474761, 474839, 476306, 476546. Nonsense mutation around 474626, 474761, 474839, 476306, 476546.", "psc": { "uniref90_id": "UniRef90_P75386", "gene": "spoT", "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "ec_ids": [ "3.1.7.2" ], "uniref50_id": "UniRef50_P75386", "cog_id": "COG0317", "cog_category": "TK", "go_ids": [ "GO:0005886", "GO:0008893", "GO:0015970" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75386", "EC:3.1.7.2", "UniRef:UniRef50_P75386", "COG:COG0317", "COG:TK", "GO:0005886", "GO:0008893", "GO:0015970" ] }, "pscc": { "uniref50_id": "UniRef50_P75386", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75386" ], "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase" } }, "id": "CNOCLNGIAL_865", "locus": "GCA000143945_00844" }, { "type": "cds", "sequence": "contig_1", "start": 476315, "stop": 476548, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAACCCATTAAGTTCGCTTTATCACAAGCTGTGTTCCAAAACTCTTACTTTGAACAAATTGAGGGAATTTACATCACCAAAATTGTGATTGAAACCTGTTGCACAAAGATTCCTGACATGCCAGAACAAGTTATTGGCATCTTAATGAAAAACATTCTCAGGGTACACCTTCATGATTGTAGGGAATTAGCTAATCAAAAGCAACCCAAAATTATTCCACTTTATTGA", "aa": "MIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCTKIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKIIPLY", "aa_hexdigest": "694f63dd98f8263921d95be02114cff8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8959.653899999994, "isoelectric_point": 8.504556465148926 }, "id": "CNOCLNGIAL_866", "locus": "GCA000143945_00845" }, { "type": "cds", "sequence": "contig_1", "start": 476567, "stop": 476803, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGACCACGTAAATTCCGCTGTCAAATTAACATTCGCGGTGTATGGAGTGAAACAACGGTCAATAAAATTGTTCAAACCATTATTGAAGGTGATAGCTATTTAGAACGAATTATTCCCAAGATCGATAAACAAAAAGATGAGTTTGAGCTCAACATTACTATGTTTATCGACAACTATCACCAACTAATCACAATTATGGAGCAGATTACTACTAAGAATATTAGCTACGTTTGA", "aa": "MRPRKFRCQINIRGVWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQITTKNISYV", "aa_hexdigest": "ed0906eba75f04ab6eb63e460804548d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9362.786699999993, "isoelectric_point": 7.868124580383301 }, "id": "CNOCLNGIAL_867", "locus": "GCA000143945_00846" }, { "type": "cds", "sequence": "contig_1", "start": 476812, "stop": 477468, "strand": "-", "frame": 3, "gene": null, "product": "putative MG279-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014325509.1", "SO:0001217", "UniParc:UPI0001DD63D2", "UniRef:UniRef100_A0AAV5N8B0", "UniRef:UniRef50_P47521", "UniRef:UniRef90_P75385" ], "nt": "ATGTTTAAGTTTTTAAAAAAGCTTTCCACTTTCCTAATTGTTTTGATTGGTATTCTACTTGTTGGTGGCATTACAGCAGCGGGTTACTTTGCCTTTGAAAACCGTGAACCTATTAATAACTATTACAAGGAAGGTTATAACAAGGTTAAACAATATAACGAGGAAATTAAAAAAGTTTCAAAGTCACTTTCTAGTAACGAACTTGTAAAAACGTTAGGTGATGTTGAAAGTTCAATTAAAGAAGGAAAAGAATTGACAAAATTACTGGATGACAGTGCTTTAGAAAGCAGTTTTAACCAACTCGAAGATTCATTAAGCAAGGTTAACAACTTCTCAAAAGGATCTACTTTTACTGAAGTTAAGAACACAATTGAAAAGATTAATCAATACGTGGACGAAATCTTAAAACGTTTTCCAAATCCTAACGAAAATGATCAGTTCAAGGAATACGTAACTAATATTTCACAAATTGTCTTTTATGTCGGGGTATCTATTATTGGTACATTTGTTGTTTCTGGTGCCTTATTGTTTATTTTCACCAAACGCGTTTATGGGGTACGAGTATCGCGCTTTAACCCACAAAGGCTATTGAAGAAACACTTAGTGTTACTTTTACAAAAGAACCAAGATGTTTATGACGAAGTATTTGAAAGTTAA", "aa": "MFKFLKKLSTFLIVLIGILLVGGITAAGYFAFENREPINNYYKEGYNKVKQYNEEIKKVSKSLSSNELVKTLGDVESSIKEGKELTKLLDDSALESSFNQLEDSLSKVNNFSKGSTFTEVKNTIEKINQYVDEILKRFPNPNENDQFKEYVTNISQIVFYVGVSIIGTFVVSGALLFIFTKRVYGVRVSRFNPQRLLKKHLVLLLQKNQDVYDEVFES", "aa_hexdigest": "8195bf9a6b05dd97d88947838b5de441", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D2", "ncbi_nrp_id": "WP_014325509.1", "uniref100_id": "UniRef100_A0AAV5N8B0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D2", "RefSeq:WP_014325509.1", "UniRef:UniRef100_A0AAV5N8B0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N8B0", "uniref90_id": "UniRef90_P75385" }, "psc": { "uniref90_id": "UniRef90_P75385", "product": "Uncharacterized protein MG279 homolog", "uniref50_id": "UniRef50_P47521", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47521", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47521" ], "product": "Uncharacterized protein MG279" }, "genes": [], "id": "CNOCLNGIAL_868", "locus": "GCA000143945_00847" }, { "type": "cds", "sequence": "contig_1", "start": 477468, "stop": 478331, "strand": "-", "frame": 1, "gene": null, "product": "putative MG280-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014325510.1", "SO:0001217", "UniParc:UPI0001DD63D3", "UniRef:UniRef100_A0AAV5NB22", "UniRef:UniRef50_P75384", "UniRef:UniRef90_P75384" ], "nt": "ATGCTCCTTTTTATTAATCGCTTTGCCAAAACAATCATTCTGTTCTTCGGGATGCTTGTCTTTTTGGTTTTGTTAGGTCTTGGCGGTGCTGCACTTTACTTTAAAGACAACGCAGCTAAACTTTACATTGATACCCGCAAATCGATCGACAGTTCCTTTGACTCTTCTCAAGCGTTTATAGATACTTATAATGGAAGTTCCAGTAAGTTTAGTGTTGAATCCATTAATAAACAAATAGAAGAGGTTAAGAAGAAAGTTGAAGAATCAACTAAAAAGTTAGAGGAGTACGAAAAGCAAATAAACCAAGCTAAAGGCTTAAACGGCTATCTTGTGAGTCCGGAAAAACTAAAGGAGTTACAAGAAGCTAAGAAGAGTCTACAAGCTACTAAGAGTCAAATAGAAAAGTATGCAAATACTTTAAAAACCGCTAATAATGGTAAAACTGGTCAAAATGGCACATCTTCTTCGACTATTCCAATTACTAAGATTTCTGGTTCAACAATTAGTGTAAGTACCAGAGATACTAACGGTAAAACCAATAGTGCTTTGAAAGATATTCAAGAGTTCTCTACACAAGCAAATGACATCATTAAACAGTACAAAGAAATTAAAAACAAAATTCCAACAGAAAAACAGTTTAATGAATATTACACCATAGGCGCAATTACCTTAGTTAGTGTTTCTGGTGGCGTTTTAGCAGTGCTAATAGTCTCTACTGTAATGACCTTCTTAGGTTCGAAAAAGTTAGGACTTAGAACGTTTTCGCGTTTAACCAGTACTGATCAAATTGCTGATCATGTTAATGACATCTTAGATCGTTATCCAGAGTTGGAAGATGCTGTTTTAGAGGAGCTTGATCAATAA", "aa": "MLLFINRFAKTIILFFGMLVFLVLLGLGGAALYFKDNAAKLYIDTRKSIDSSFDSSQAFIDTYNGSSSKFSVESINKQIEEVKKKVEESTKKLEEYEKQINQAKGLNGYLVSPEKLKELQEAKKSLQATKSQIEKYANTLKTANNGKTGQNGTSSSTIPITKISGSTISVSTRDTNGKTNSALKDIQEFSTQANDIIKQYKEIKNKIPTEKQFNEYYTIGAITLVSVSGGVLAVLIVSTVMTFLGSKKLGLRTFSRLTSTDQIADHVNDILDRYPELEDAVLEELDQ", "aa_hexdigest": "0342094c0c3ceb97afe2967795ea16bc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D3", "ncbi_nrp_id": "WP_014325510.1", "uniref100_id": "UniRef100_A0AAV5NB22", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D3", "RefSeq:WP_014325510.1", "UniRef:UniRef100_A0AAV5NB22" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NB22", "uniref90_id": "UniRef90_P75384" }, "psc": { "uniref90_id": "UniRef90_P75384", "product": "Uncharacterized protein MG280 homolog", "uniref50_id": "UniRef50_P75384", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75384", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75384" ], "product": "Uncharacterized protein MG280 homolog" }, "genes": [], "id": "CNOCLNGIAL_869", "locus": "GCA000143945_00848" }, { "type": "cds", "sequence": "contig_1", "start": 478393, "stop": 478788, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22806.1", "PFAM:PF22805.1" ], "nt": "ATGTACCGGGTGAACGAAAATTACTATCCGGGTGCCAGTGCTTACGAAGCGGAACGCGCTACCCGTGATAGTGAGTTCCAAAAGGAAATTGTACAACGCGCTGAACTTATTGGGGTTGTCTTTGAATATGGAGTGAAAAACCTCCGTCCGGGTTTAAAGTACACCGTGAAGTTTGAAAGTCCACAAGAACAAGTGGCATTAAAGAGTACCGATAAGTTCCAACCAGTTATTGGTAGTGTCACTGATATGAGCAAATCAGTTACTGATTTAATCGGTGTATTACGTGATAACGCCGAAATTCTCAATATTACTAATGTTTCTAAAGATGAAACCGTTGTAGCGGAATTGAAAGAAAAATTGGATCGGGAAAATGTTTTCCAAGAGATTCGAACTTAA", "aa": "MYRVNENYYPGASAYEAERATRDSEFQKEIVQRAELIGVVFEYGVKNLRPGLKYTVKFESPQEQVALKSTDKFQPVIGSVTDMSKSVTDLIGVLRDNAEILNITNVSKDETVVAELKEKLDRENVFQEIRT", "aa_hexdigest": "0ab28e9612b4b152aa7a9bbec4443559", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22806.1", "name": "M_C", "length": 99, "aa_cov": 0.7480916030534351, "hmm_cov": 1.0, "evalue": 3.766603360991261e-54, "score": 176.7239990234375, "start": 34, "stop": 131 }, { "id": "PF22805.1", "name": "M_smaller_dom", "length": 28, "aa_cov": 0.21374045801526717, "hmm_cov": 1.0, "evalue": 8.076161373520137e-23, "score": 75.56156158447266, "start": 6, "stop": 33 } ], "seq_stats": { "molecular_weight": 14938.667299999985, "isoelectric_point": 5.003786659240721 }, "id": "CNOCLNGIAL_870", "locus": "GCA000143945_00849" }, { "type": "cds", "sequence": "contig_1", "start": 478926, "stop": 479768, "strand": "-", "frame": 1, "gene": null, "product": "IgG-blocking protein M", "db_xrefs": [ "GO:0005515", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47523", "UniRef:UniRef90_P75383" ], "nt": "GTGCCTAAGGTAGAGGTGAGCGGTTTGATTAAAACCAGTTCTACCTTTATCACGCCAAACTTTAAAGCGGGTTACTACGACCAAGTAGCAGCGGACGGGAAAACGCTCAAATATTACCAATCAACCGAATACTTTAACAACCGGGTAGTAATGCCAATTTTGCAAACTACCAACGGCACTTTAACTGCTAACAATAGGGCTTACGATGATATCTTTGTCGATCAAGGTGTCCCTAAGTTCCCTGGTTGGTTCCATGACGTGGATAAGGCTTACTATGCTGGCTCCAATGGACAAAGCGAGTACTTGTTTAAAGAGTGGAATTACTATGTTGCTAACGGTTCACCGTTATACAACGTTTACCCAAACCATCACTTTAAGCAAATAAAAACGATTGCCTTTGATGCTCCAAGGATTAAGCAAGGTAATACTGACGGCATTAACCTCAATTTAAAACAACGTAATCCTGATTACGTCATCATTAATGGTTTAACTGGTGATGGTTCTACCTTAAAGGATTTGGAATTACCAGAAAGCGTTAAAAAGGTGTCAATTTATGGTGACTACCACTCGATTAATGTTGCTAAGCAAATTTTTAAAAACGTGTTAGAGTTGGAGTTTTACTCTACTAACCAAGACAATAACTTTGGTTTTAATCCACTAGTTTTAGGCGATCACACTAATATTATCTATGACCTTTTTGCTTCCAAGCCGTTTAATTACATTGATTTAACTAGTTTAGAACTTAAAGATAACCAAGACAATATTGATGCATCTAAGTTAAAGCGTGCTGTTAGTGACATTTACATTCGTCGTCGTTTTGAACGACAAATGCAAGGTTACTGA", "aa": "MPKVEVSGLIKTSSTFITPNFKAGYYDQVAADGKTLKYYQSTEYFNNRVVMPILQTTNGTLTANNRAYDDIFVDQGVPKFPGWFHDVDKAYYAGSNGQSEYLFKEWNYYVANGSPLYNVYPNHHFKQIKTIAFDAPRIKQGNTDGINLNLKQRNPDYVIINGLTGDGSTLKDLELPESVKKVSIYGDYHSINVAKQIFKNVLELEFYSTNQDNNFGFNPLVLGDHTNIIYDLFASKPFNYIDLTSLELKDNQDNIDASKLKRAVSDIYIRRRFERQMQGY", "aa_hexdigest": "94e1cc0758c17af4793dcbe08767d015", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 478624, "stop": 480068, "observations": { "deletion": [ 478880 ], "mutation": [ 478928 ], "stop": [ 478928 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75383", "query_cov": 1.0, "subject_cov": 0.48109965635738833, "identity": 0.996, "score": 570.0, "evalue": 6.89e-201, "gene_start": 1, "gene_end": 280, "reference_start": 125, "reference_end": 404, "reference_sequence": "MKLNFKIKDKKTLKRLKKGGFWALGLFGAAINAFSAVLIVNEVLRLQSGETLIASGRSGNLSFQLYSKVNQNAKSKLNSISLTDGGYRSEIDLGDGSNFREDFRNFANNLSEAITDAPKDLLRPVPKVEVSGLIKTSSTFITPNFKAGYYDQVAADGKTLKYYQSTEYFNNRVVMPILQTTNGTLTANNRAYDDIFVDQGVPKFPGWFHDVDKAYYAGSNGQSEYLFKEWNYYVANGSPLYNVYPNHHFKQIKTIAFDAPRIKQGNTDGINLNLKQRNPDYVIINGLTGDGSTLKDLELPESVKKVSIYGDYHSINVAKQIFKNVLELEFYSTNQDNNFGFNPLVLGDHTNIIYDLFASKPFNYIDLTSLELKDNQDNIDASKLKRAVSDIYIRRRFERQMQGYWAGGYIDRYLVKNTNEKNVNKDNDTVYAALKDINLHLEETYTHGGNTMYRVNENYYPGASAYEAERATRDSEFQKEIVQRAELIGVVFEYGVKNLRPGLKYTVKFESPQEQVALKSTDKFQPVIGSVTDMSKSVTDLIGVLRDNAEILNITNVSKDETVVAELKEKLDRENVFQEIRT" }, "paralog": false, "identity": 0.997920997920998, "score": 962.0, "evalue": 0.0, "description": "Internal stop codon at 478928. Frameshift due to deletion around 478880. Nonsense mutation around 478928.", "psc": { "uniref90_id": "UniRef90_P75383", "product": "IgG-blocking protein M", "uniref50_id": "UniRef50_P47523", "go_ids": [ "GO:0005515", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75383", "UniRef:UniRef50_P47523", "GO:0005515", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47523" ], "product": "Uncharacterized protein MG281" } }, "id": "CNOCLNGIAL_871", "locus": "GCA000143945_00850" }, { "type": "tRNA", "sequence": "contig_1", "start": 480428, "stop": 480501, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 74.9, "nt": "GCGAGTATAGTTTAGTGGTAGAACATCAGCCTTCCAAGCTGATCGTGTCGGTTCGATTCCGATTACTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 480467, 480469 ], "id": "CNOCLNGIAL_30", "locus": "GCA000143945_00851" }, { "type": "tRNA", "sequence": "contig_1", "start": 480508, "stop": 480593, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 70.5, "nt": "GCCCAAGTGGCGGAATGGTAGACGCATGGGATTTAAGATCCCACGCTAGCAATAGCGTGCCGGTTCAAGTCCGGCTTTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 480558, 480560 ], "id": "CNOCLNGIAL_29", "locus": "GCA000143945_00852" }, { "type": "tRNA", "sequence": "contig_1", "start": 480594, "stop": 480668, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 77.0, "nt": "GACTCACTAGCTCAGCGGTAGAGCATTTGACTTTTAATCAAAGGGTCCCGAGTTCGATCCTCGGGTGAGTCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 480641, 480643 ], "id": "CNOCLNGIAL_28", "locus": "GCA000143945_00853" }, { "type": "tRNA", "sequence": "contig_1", "start": 480712, "stop": 480786, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 70.6, "nt": "TGGGATGTAGCCAAGCGGTAAGGCAATAGACTTTGACTCTATCATGCGATGGTTCGATCCCATCCATCCCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 480752, 480754 ], "id": "CNOCLNGIAL_27", "locus": "GCA000143945_00854" }, { "type": "tRNA", "sequence": "contig_1", "start": 480792, "stop": 480878, "strand": "-", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 72.9, "nt": "GGACAGGTAGCGAAGTGGCTAAACGCTTCTGACTGTAGATCAGACACCTTCATGGTTTCGGGAGTTCGAATCTCTCCCTGTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 480870, 480872 ], "id": "CNOCLNGIAL_26", "locus": "GCA000143945_00855" }, { "type": "cds", "sequence": "contig_1", "start": 480908, "stop": 481390, "strand": "-", "frame": 2, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063", "RefSeq:WP_014325511.1", "SO:0001217", "UniParc:UPI0001DD63D4", "UniRef:UniRef100_A0AAX0SP23", "UniRef:UniRef50_P47524", "UniRef:UniRef90_P78019" ], "nt": "ATGGAACTTAACAAAAATTACCTTACCGAAGAGGGGCTAAAACAATTAGAAGCGGAGCTGGAACACCTCATTCAAGTAAAACGTCCCGCTATTATTAAGTTATTGCAAGAAGCCCGTGACCAGGGTGACTTGAGTGAAAACGCTGATTACGATGCAGCAAAAGCACAGCAGGGTGAAATTGAAACCCGCATTGCTGAAATCCAAGACATTTTAGCCAACGTTAAGTTAATTAATGAGTCGCAAACAAAAAAGGCTAATAAGGTTACCCTGGGTAGTACGGTCGAAATTTACGATTACAGTTCGAAAACACACGAAAAGTACACCATTGTGGGAGCTTTAGAAGCTAATCCCGAAGAACACCGCATCTCCAATGAATCCCCTTTGGCACACGCCATTTATGGTCGTTTAGTGGATGATGAATGTGACGTAGTTGGCATAGAAGTGCCCTACCGGGTCAAAATTGTTAAGATCATTAACGGATAA", "aa": "MELNKNYLTEEGLKQLEAELEHLIQVKRPAIIKLLQEARDQGDLSENADYDAAKAQQGEIETRIAEIQDILANVKLINESQTKKANKVTLGSTVEIYDYSSKTHEKYTIVGALEANPEEHRISNESPLAHAIYGRLVDDECDVVGIEVPYRVKIVKIING", "aa_hexdigest": "3f77c1d22e63dd1d57a5b5856630c969", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D4", "ncbi_nrp_id": "WP_014325511.1", "uniref100_id": "UniRef100_A0AAX0SP23", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D4", "RefSeq:WP_014325511.1", "UniRef:UniRef100_A0AAX0SP23" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SP23", "uniref90_id": "UniRef90_P78019" }, "psc": { "uniref90_id": "UniRef90_P78019", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_P47524", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_P47524", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47524" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "CNOCLNGIAL_872", "locus": "GCA000143945_00856" }, { "type": "cds", "sequence": "contig_1", "start": 481520, "stop": 481660, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574968.1", "SO:0001217", "UniParc:UPI0001DD63D5", "UniRef:UniRef100_A0AB38W775", "UniRef:UniRef50_A0AB38W775", "UniRef:UniRef90_A0AB38W775" ], "nt": "ATGACAACGAAAGACAATTCTGGCATTAAAAGTAAAAGACGGATTCGTTTTTTAGCTTTAGGAATTATTATTTTGATTCTTGTAATTGCTGTAATCACCATTTTATTTGTCACGGGTGTAATTACCCGCGCTAACCAATAA", "aa": "MTTKDNSGIKSKRRIRFLALGIIILILVIAVITILFVTGVITRANQ", "aa_hexdigest": "86e8640e47c62ed2fa104c8b81b0bc6b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D5", "ncbi_nrp_id": "WP_014574968.1", "uniref100_id": "UniRef100_A0AB38W775", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D5", "RefSeq:WP_014574968.1", "UniRef:UniRef100_A0AB38W775" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W775", "uniref90_id": "UniRef90_A0AB38W775" }, "psc": { "uniref90_id": "UniRef90_A0AB38W775", "uniref50_id": "UniRef50_A0AB38W775" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W775" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5082.190099999999, "isoelectric_point": 11.999967765808105 }, "id": "CNOCLNGIAL_873", "locus": "GCA000143945_00857" }, { "type": "cds", "sequence": "contig_1", "start": 481896, "stop": 482120, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGCAAGTTCAACTACCGACCCTTTCCTTTTACAGTGAGTACCAAAAGGAAAAGCGTCATGTGGAGGGCTTTAATCCTGAGCTTTTTTTAATAGAGCAAATAGGTACTAAAAAACTCCATGATCCGCTAGTACTACGTCCTACTAGTGAAATTGCTTTTTGTAACTTATGGAAAAAACAGAGCTTTTCTTACCAAAACCTTCCCGTTATCTATAACCAGTGA", "aa": "MEQVQLPTLSFYSEYQKEKRHVEGFNPELFLIEQIGTKKLHDPLVLRPTSEIAFCNLWKKQSFSYQNLPVIYNQ", "aa_hexdigest": "3447f991c38ca5aaebaa72b61424f513", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8832.037399999997, "isoelectric_point": 6.714697074890137 }, "id": "CNOCLNGIAL_874", "locus": "GCA000143945_00858" }, { "type": "cds", "sequence": "contig_1", "start": 482361, "stop": 482558, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCGATGGGCAGGCATTGCAGTGTGCCACGAGCCATGATTTAGGGCAAACCTTTACCAAACCGTTTGGGCTAACCTTTCAAAACCAAGCCAACCAACAGGCCATTCCCTACAGCTTTAGTTGTGGAATTTCTACAAGAATCCTCGGTGCTTTACTGTTAACCCACAGTGATGACTTTGGACTGATACTACCATGA", "aa": "MPDGQALQCATSHDLGQTFTKPFGLTFQNQANQQAIPYSFSCGISTRILGALLLTHSDDFGLILP", "aa_hexdigest": "89de8a3476faefd9ce5bed9b655b4eec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6997.851899999999, "isoelectric_point": 5.18828601837158 }, "id": "CNOCLNGIAL_875", "locus": "GCA000143945_00859" }, { "type": "cds", "sequence": "contig_1", "start": 482637, "stop": 483122, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09180.16" ], "nt": "GTGCAAACGTTGTTGGAACAATTAGCAATCCGTTTTCAATTTATTAAGGTCGAAAACCAGCTCGGTAAACAGTTAGGACAGGGTGAAGTCAACGGCATTCCCTTTCAACTAATAGTTGACAATCCCCAAACAGTCAACATCTTTAACCGGTTAACCAGGGTCAAAACAGCTTACAGTTTCGAACAGTTGGCAAGCCGATTTGTGGAGCTCGTGCAACAGTACCATCAAGCAATGTATGACAAAGCCAAAGCAGTGGTACAGCAAAAGGTGGTGCAAGCCACTACTTTAAAGCAAATTGAACAAGCCTTTAATGACAAAAAGGCTGTTTTGTGTGCGGTTCGTTTAACCGATACATTAGAACAGCAATTAAAGGAACGGTACCAGGTCACGGTGCGCTGTTGCTTAGAGCAGCTTCAAAAGCCCCAAATTTGTCCGTTTAGCGGTGAGTCAGCACAGGACTATGTCCTGATTGCGCGTGCCTATTAA", "aa": "MQTLLEQLAIRFQFIKVENQLGKQLGQGEVNGIPFQLIVDNPQTVNIFNRLTRVKTAYSFEQLASRFVELVQQYHQAMYDKAKAVVQQKVVQATTLKQIEQAFNDKKAVLCAVRLTDTLEQQLKERYQVTVRCCLEQLQKPQICPFSGESAQDYVLIARAY", "aa_hexdigest": "46cf1f9a1daf19c4c12de05acbaa0777", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09180.16", "name": "ProRS-C_1", "length": 70, "aa_cov": 0.40372670807453415, "hmm_cov": 1.0, "evalue": 3.1271991469392555e-11, "score": 38.782405853271484, "start": 97, "stop": 161 } ], "seq_stats": { "molecular_weight": 18543.232399999994, "isoelectric_point": 8.897168922424317 }, "id": "CNOCLNGIAL_876", "locus": "GCA000143945_00860" }, { "type": "cds", "sequence": "contig_1", "start": 483267, "stop": 483635, "strand": "+", "frame": 3, "gene": null, "product": "putative MG284-like protein", "db_xrefs": [ "RefSeq:WP_010874759.1", "SO:0001217", "UniParc:UPI0000139587", "UniRef:UniRef100_P75381", "UniRef:UniRef50_P47526", "UniRef:UniRef90_P75381" ], "nt": "ATGAGTTTTAAAGTTAAAAACCAGAGTAAGCACCTATACTCTTTAATGACGAAATTTAAACGTTCGCAACTGATCTTGAAGCACCAAAGCAATAACTTTGCCTCAGAGTTGTGGAACGAAGAGGACATTATCCGCTCCAAGCAGTTTATCGAACTTATAGAAGACACGCTGCTCCACCTCAAGAAGGATACGGTCGACTTTATTTACGACATCTTCATTTATGGGAAGAAGCCGTGTGATATTAGTTACTCCAATTCGACCTATTACAAAAAGTTAAACAAGGCTGCAAATAGTTTTTTTGACCATTTTGTCTGGGAGGCTCCAATTTATAAGACAAAGGAGCTTAAAAATGACAATTCTCACAGTTAG", "aa": "MSFKVKNQSKHLYSLMTKFKRSQLILKHQSNNFASELWNEEDIIRSKQFIELIEDTLLHLKKDTVDFIYDIFIYGKKPCDISYSNSTYYKKLNKAANSFFDHFVWEAPIYKTKELKNDNSHS", "aa_hexdigest": "3bb59ed69b6a3d1cb6f566a83d959a59", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139587", "ncbi_nrp_id": "WP_010874759.1", "uniref100_id": "UniRef100_P75381", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139587", "RefSeq:WP_010874759.1", "UniRef:UniRef100_P75381" ] }, "ips": { "uniref100_id": "UniRef100_P75381", "uniref90_id": "UniRef90_P75381" }, "psc": { "uniref90_id": "UniRef90_P75381", "product": "Uncharacterized protein MG284 homolog", "uniref50_id": "UniRef50_P47526" }, "pscc": { "uniref50_id": "UniRef50_P47526", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47526" ], "product": "Uncharacterized protein MG284" }, "genes": [], "id": "CNOCLNGIAL_877", "locus": "GCA000143945_00861" }, { "type": "cds", "sequence": "contig_1", "start": 483616, "stop": 483846, "strand": "+", "frame": 1, "gene": null, "product": "DUF5443 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HMP7", "UniRef:UniRef90_A0AB33HMP7" ], "nt": "ATGACAATTCTCACAGTTAGACAAATTCAAAATAATTACTATACGGAATACTACATGGGTCCGGTTAGAAACTTTGCTGACAAAAAGGAAGTTTACTTTGATGCTAAGGTAAACAATATTGAAAGCAAAGTCAACAAGGATAAAGCCTTGTTACACATAACCCTGGCGTTAAAGTACGACAGTAACTTTCCCAGTAATATGTTCCAAGCTCAGTTTAAACTGGGTAATTGA", "aa": "MTILTVRQIQNNYYTEYYMGPVRNFADKKEVYFDAKVNNIESKVNKDKALLHITLALKYDSNFPSNMFQAQFKLGN", "aa_hexdigest": "3db421df654ed2b4b39175648bdcad95", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 483616, "stop": 483999, "observations": { "mutation": [ 483844 ], "stop": [ 483844 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HMP7", "query_cov": 1.0, "subject_cov": 0.5984251968503937, "identity": 1.0, "score": 154.0, "evalue": 6.73e-47, "gene_start": 1, "gene_end": 76, "reference_start": 1, "reference_end": 76, "reference_sequence": "MTILTVRQIQNNYYTEYYMGPVRNFADKKEVYFDAKVNNIESKVNKDKALLHITLALKYDSNFPSNMFQAQFKLGNWSSEKIPLQKAPDKNPIWKIRFTIFMPLWKCHELPLLNGKLTALVSIFVKN" }, "paralog": false, "identity": 0.9921259842519685, "score": 258.0, "evalue": 1.48e-90, "description": "Internal stop codon at 483844. Nonsense mutation around 483844.", "psc": { "uniref90_id": "UniRef90_A0AB33HMP7", "product": "Family of unknown function (DUF5443)", "uniref50_id": "UniRef50_A0AB33HMP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HMP7", "UniRef:UniRef50_A0AB33HMP7" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HMP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HMP7" ] } }, "id": "CNOCLNGIAL_878", "locus": "GCA000143945_00862" }, { "type": "cds", "sequence": "contig_1", "start": 483933, "stop": 484193, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCACGAACTGCCCTTATTAAACGGGAAATTAACCGCATTAGTGAGTATATTCGTGAAGAATTAACAATTGCTTTTCGCTTAAATGACAGTAGTGATCGCTACCATTGGAGTAACTATAACCTGTTCGATACAGTGGCAGCTGATATGTACCAAACGGTCATTAAAGAAACCGTTACCTTTGGTAACATGATTAGACTAAACCAACTCGAGGGCGAGAAGGAAGGTAACATCCAACAAAGTGAACTTAAATATGGTTGA", "aa": "MPRTALIKREINRISEYIREELTIAFRLNDSSDRYHWSNYNLFDTVAADMYQTVIKETVTFGNMIRLNQLEGEKEGNIQQSELKYG", "aa_hexdigest": "9a95947fe0086d9d3decd8c19b46e65b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10178.338500000002, "isoelectric_point": 5.350277137756348 }, "id": "CNOCLNGIAL_879", "locus": "GCA000143945_00863" }, { "type": "cds", "sequence": "contig_1", "start": 484197, "stop": 484655, "strand": "+", "frame": 3, "gene": null, "product": "DUF5443 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W764", "UniRef:UniRef90_A0AB38W764" ], "nt": "ATGTTGGACTACCGCAATATGGGGCCGCTGGAAGAGGTTAATAATTTAATTAATATTAAGTTTAACCAACCAGTAAAAGTGGTAAGTGTTGGTGTGACCGTAAGCTATACAGCGCCTGATGGTAAACGCCAAGAGCTCAAAGATCAAGCTGAATACCAGCACACCCTCAACCCCAATGAAGAGTTCAAACTGAACTTTCCCAGACGGCTAAGCTTTAACAGGGTAACTAAAAAGTTGGACTTTGACCCCAATGGTAGTGCCGTGTTCCTACCACAGGGTGGCTTTGGTAGCTATGAGATCAAACTGGAGGCTAATGTCGGCAACCAGTTCTACACAATTGTGGCTACTAACAGCTTTGAGTACGCCCATCCGTTTGACGATCCCAAGACCAATGACTTCTTTCTGGTGCAGTACGCTCCAGTCTACAGTCTGTTTAACTTTAGCGATTTAATTCAGTAA", "aa": "MLDYRNMGPLEEVNNLINIKFNQPVKVVSVGVTVSYTAPDGKRQELKDQAEYQHTLNPNEEFKLNFPRRLSFNRVTKKLDFDPNGSAVFLPQGGFGSYEIKLEANVGNQFYTIVATNSFEYAHPFDDPKTNDFFLVQYAPVYSLFNFSDLIQ", "aa_hexdigest": "e0cfce36bda916afae4f31fcb9de000c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 483933, "stop": 484655, "observations": { "mutation": [ 484191 ], "stop": [ 484191 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W764", "query_cov": 1.0, "subject_cov": 0.6333333333333333, "identity": 1.0, "score": 311.0, "evalue": 3.25e-106, "gene_start": 1, "gene_end": 152, "reference_start": 89, "reference_end": 240, "reference_sequence": "MPRTALIKREINRISEYIREELTIAFRLNDSSDRYHWSNYNLFDTVAADMYQTVIKETVTFGNMIRLNQLEGEKEGNIQQSELKYGWTMLDYRNMGPLEEVNNLINIKFNQPVKVVSVGVTVSYTAPDGKRQELKDQAEYQHTLNPNEEFKLNFPRRLSFNRVTKKLDFDPNGSAVFLPQGGFGSYEIKLEANVGNQFYTIVATNSFEYAHPFDDPKTNDFFLVQYAPVYSLFNFSDLIQ" }, "paralog": false, "identity": 0.9916666666666667, "score": 482.0, "evalue": 5.43e-176, "description": "Internal stop codon at 484191. Nonsense mutation around 484191.", "psc": { "uniref90_id": "UniRef90_A0AB38W764", "product": "Family of unknown function (DUF5443)", "uniref50_id": "UniRef50_A0AB38W764", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W764", "UniRef:UniRef50_A0AB38W764" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W764", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W764" ] } }, "id": "CNOCLNGIAL_880", "locus": "GCA000143945_00864" }, { "type": "cds", "sequence": "contig_1", "start": 484662, "stop": 485255, "strand": "+", "frame": 3, "gene": null, "product": "putative MG286-like protein", "db_xrefs": [ "GO:0016020", "RefSeq:WP_014325515.1", "SO:0001217", "UniParc:UPI0001DD63D7", "UniRef:UniRef100_A0AAX0SP07", "UniRef:UniRef50_P47528", "UniRef:UniRef90_P75379" ], "nt": "ATGATCTTCTCAATTAGTAAACGCAAGTTAATTTGTGGCTTTTCCCTAGTAGCCCTTACAATCGCTGGGATTGTTGGTGGGGTTTACTTAGTCACCAAAAACAATCAACAAACCACACCACAAACAAATCACTTTAATGTAGCTGAACCAGTCAACAAGGTCCCTAATTGGCGCAAACTAGGTCCAGAAACACAACGCGAACTGCGCGATCGTCTGTATCCCTTAGATGACACAGGTTACTTTATTTACAAGTACGGTGCCATTAGTCGCTACCTCCAAAGCCAAAAGGAACTGGATGAACTAGTCGATTACCGCACCGTGTTACCCTCCACCCAAAAGCACTTTAAGTATGATTCCTTTAACCAAAGCGTGTTGGAAAGTAAGCTGCGTAAGTGGTTAATGAAAGCCATTAAACAACACCCGTACTTTCAGCACTTTGAGTTTGATCCGGTGCTTAAAGCACAGTACAATATCAACATTCCTGCTCAAAAAATTACGGTGAATGCAGTTTGGTTTTACAAAAAGGATAATGATCTCACCACGGGCAAGCCAATCCGCTATTGGGATCAGTTTGAAATTAAACTAAAGCAGTAG", "aa": "MIFSISKRKLICGFSLVALTIAGIVGGVYLVTKNNQQTTPQTNHFNVAEPVNKVPNWRKLGPETQRELRDRLYPLDDTGYFIYKYGAISRYLQSQKELDELVDYRTVLPSTQKHFKYDSFNQSVLESKLRKWLMKAIKQHPYFQHFEFDPVLKAQYNINIPAQKITVNAVWFYKKDNDLTTGKPIRYWDQFEIKLKQ", "aa_hexdigest": "5bce37a824b340df385a2057fdad818c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D7", "ncbi_nrp_id": "WP_014325515.1", "uniref100_id": "UniRef100_A0AAX0SP07", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D7", "RefSeq:WP_014325515.1", "UniRef:UniRef100_A0AAX0SP07" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SP07", "uniref90_id": "UniRef90_P75379" }, "psc": { "uniref90_id": "UniRef90_P75379", "product": "Uncharacterized protein MG286 homolog", "uniref50_id": "UniRef50_P47528", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47528", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47528" ], "product": "Uncharacterized protein MG286" }, "genes": [], "id": "CNOCLNGIAL_881", "locus": "GCA000143945_00865" }, { "type": "tRNA", "sequence": "contig_1", "start": 485535, "stop": 485609, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 70.7, "nt": "AGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCA", "db_xrefs": [], "id": "CNOCLNGIAL_12", "locus": "GCA000143945_00866" }, { "type": "cds", "sequence": "contig_1", "start": 485799, "stop": 485942, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SQH8", "UniRef:UniRef90_A0AAX0SQH8" ], "nt": "GTGGATGATCAAACCGGTGTGCTCGACATTAAGCAGTCTGTACCACCCAAACTAAAGGTTAACAAACAAGGCTTTGAACCTGTTTTCATCACCTTTACTAACACTAACAACAACGAGCAAGTTACCGTGGTTTTAAACAACTGA", "aa": "MDDQTGVLDIKQSVPPKLKVNKQGFEPVFITFTNTNNNEQVTVVLNN", "aa_hexdigest": "4d88cce42c741a7f190bb9682c1e8a27", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 485649, "stop": 485942, "observations": { "mutation": [ 485762, 485792, 485801 ], "stop": [ 485762, 485792, 485801 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0SQH8", "query_cov": 1.0, "subject_cov": 0.4845360824742268, "identity": 1.0, "score": 95.5, "evalue": 1.17e-24, "gene_start": 1, "gene_end": 47, "reference_start": 1, "reference_end": 47, "reference_sequence": "MDDQTGVLDIKQSVPPKLKVNKQGFEPVFITFTNTNNNEQVTVVLNNWSQWETHKTDFAPWLKCKHWNIDVKQIRRVKTTFRDQENANFFELIESKQ" }, "paralog": false, "identity": 0.9587628865979382, "score": 189.0, "evalue": 6.12e-64, "description": "Internal stop codon at 485762, 485792, 485801. Nonsense mutation around 485762, 485792, 485801.", "psc": { "uniref90_id": "UniRef90_A0AAX0SQH8", "uniref50_id": "UniRef50_A0AAX0SQH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0SQH8", "UniRef:UniRef50_A0AAX0SQH8" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SQH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SQH8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5275.897100000002, "isoelectric_point": 4.8610639572143555 }, "id": "CNOCLNGIAL_882", "locus": "GCA000143945_00867" }, { "type": "cds", "sequence": "contig_1", "start": 486030, "stop": 486200, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCTTACAGTTAGCTAAAGTGAGTGGGATTGATATTAATGAAAACAAACTGTTTAACACTGATTTTTTAAAGAAGTCGTTTTACTTTGCCGATAAATTTTCCCACCTTTCAGTGAACGGTAATGTGGAATACATGCGCAATATCTTAACAACTGTTGGGTTTGATTGA", "aa": "MFLQLAKVSGIDINENKLFNTDFLKKSFYFADKFSHLSVNGNVEYMRNILTTVGFD", "aa_hexdigest": "1c83aba5ab5176eff1197662a976d41e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6482.352200000001, "isoelectric_point": 6.53480167388916 }, "id": "CNOCLNGIAL_883", "locus": "GCA000143945_00868" }, { "type": "cds", "sequence": "contig_1", "start": 486282, "stop": 486554, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13472.12" ], "nt": "GTGATCAACAGTGCCATTAATGGTAGTAACACCTCCTTAGAGTTGTACATGCAAAATCATCGCTTTAAGCAGTACAACAACATTGATGTTTATGTACTTAATTTGGGTACTAATGACATTAACCAGTTAGCGCAAGGGGTTTACACAGTCGAGCAATACAAACATAATCTTACCCAAATACTGGATCTGTTACATACGCAAAGTCCTCAAGCCCATGTTGTTTTAGCCAACATTGCTCCATCTAGCTTACTGCGAAGCTCTGAAAAGAACTGA", "aa": "MINSAINGSNTSLELYMQNHRFKQYNNIDVYVLNLGTNDINQLAQGVYTVEQYKHNLTQILDLLHTQSPQAHVVLANIAPSSLLRSSEKN", "aa_hexdigest": "0032984956c2e60ea87431f041e2cefe", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13472.12", "name": "Lipase_GDSL_2", "length": 80, "aa_cov": 0.8666666666666667, "hmm_cov": 0.95, "evalue": 8.233110608640638e-11, "score": 38.16858673095703, "start": 3, "stop": 80 } ], "seq_stats": { "molecular_weight": 10170.296099999994, "isoelectric_point": 6.377414512634277 }, "id": "CNOCLNGIAL_884", "locus": "GCA000143945_00869" }, { "type": "cds", "sequence": "contig_1", "start": 487019, "stop": 487333, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTGAAGACAAAGTTAGCTTAAGTTTGCAAGGAGAATTTGCGATTGGTAAACAGTTAGCACAAGTGTTAAAAGCTACTGGCGCTGAACCATTGCCAGTGGAAGTTACAGAACCATCACTAGAAGCTATACCAATTAATGAAGAAACCCAGTTTGATGTAAAAAATTACTACAGCTTTGGCCATTACCATGACACCAATGTCATTAATGACAGGATTGATTTTGATGATCCCAACTACAACTTAGAAACGGACTTTTTCAATAATGCTAAAGCTAATTTGGTAACTACTTCCAATCCCTTTACTGTTACTTGA", "aa": "MSEDKVSLSLQGEFAIGKQLAQVLKATGAEPLPVEVTEPSLEAIPINEETQFDVKNYYSFGHYHDTNVINDRIDFDDPNYNLETDFFNNAKANLVTTSNPFTVT", "aa_hexdigest": "c39648a9d1d8352e338100dbdf228006", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11644.65189999998, "isoelectric_point": 4.223273658752441 }, "id": "CNOCLNGIAL_885", "locus": "GCA000143945_00870" }, { "type": "cds", "sequence": "contig_1", "start": 487502, "stop": 487762, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCTAGTGCTCGGTTTGAAGGTAAGATAGCAAACTTTGGTTTTGCCGGGCAGACTATGAAAGATATTAATGAGAGCTTTCAAGAACGGGTACTTTCAATTTACAAACCACGTACGCTAGTTTACCTCACTTCCCAAGCGGATGCAGAGCGGGTTGATTATTACGAGGAGTTTGTCAATAATCTAACCGCACTCATTAAAAAGCAAGTTGGTGAAAAGGCTGAAAAGGACAACTTTTTCATTTTGCAATTGCACTGA", "aa": "MFASARFEGKIANFGFAGQTMKDINESFQERVLSIYKPRTLVYLTSQADAERVDYYEEFVNNLTALIKKQVGEKAEKDNFFILQLH", "aa_hexdigest": "10e8c22a9cf686b7f2745657e6e1dd78", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9974.213899999993, "isoelectric_point": 5.775148582458496 }, "id": "CNOCLNGIAL_886", "locus": "GCA000143945_00871" }, { "type": "cds", "sequence": "contig_1", "start": 487958, "stop": 488194, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCACTCAGAGTGATGTTTCACCACTCTTTGTGAAAAATATCAACATCCAACTACCGAGCTCCACCCATCAACAAAAGATTGAACCCTTAAATTTACCAACTAACATTAGCAAAACAGAAATAGCGAGTTGGGGTAGTGAATTCCAAAGCAAGGTATTGGACTTTTATGCTAGGGATGGTGAAGAACATGACAACAAAATTAGGTACCAACTCCCATCCTTAAGCTTTGCTTGA", "aa": "MGTQSDVSPLFVKNINIQLPSSTHQQKIEPLNLPTNISKTEIASWGSEFQSKVLDFYARDGEEHDNKIRYQLPSLSFA", "aa_hexdigest": "c33f8049d6badddb7a1fe65f6e5d6cd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8836.776099999992, "isoelectric_point": 5.513689231872558 }, "id": "CNOCLNGIAL_887", "locus": "GCA000143945_00872" }, { "type": "cds", "sequence": "contig_1", "start": 488515, "stop": 489147, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTAAGAAGGGTTTTTTCGCGTTATCAAGTTGCACATTAGGTCTAGGTCTTATTTTAACTGCATGTGGTGCTAGGGGTAAATTTGACCAAGTTGACGATGGTGTTATTAAGCTAGCTACTTCAATCCAAAATAAGGACAGTATCGCTGCTTTAAACACCATTTACAAGAAGTATAAGGAACGCCATCCAGGGAGTTATCCAGTTCAAAATTTTCAGGTTCCCGGTGGTTACAGTGGTTTACGGAACGACATTAGAACGCGTTTATCAGCTAAAGATGGCAACAACTTTTACAACATAGTACTCAACTATCCTGATGTTGTCTCCTCATTGGCTACAAGTGACATGCAACTAATATTGGATAACGTTGACACCAAGCTCCTTTCGAAAAATTTCTTGAGCTTTAACGAGCGAATTGGTGGTGTACGACAAAAGGGGATTTATGCAATTCCCATTTCCCTTTCCACTGAATTAATGGTGCTCAACGGACCGGTATTGCACTATATTTTGAACAGTGCTAAGAAGAAGGAAAACGGTGCTCAAATGATTAAAAGATCAGGTAGTTTTTCCACTGTTCAAAAAGGTACCATGGCAATCGACATGAATGATCAAAAAACCAAAGATCTTTGA", "aa": "MVIKKGFFALSSCTLGLGLILTACGARGKFDQVDDGVIKLATSIQNKDSIAALNTIYKKYKERHPGSYPVQNFQVPGGYSGLRNDIRTRLSAKDGNNFYNIVLNYPDVVSSLATSDMQLILDNVDTKLLSKNFLSFNERIGGVRQKGIYAIPISLSTELMVLNGPVLHYILNSAKKKENGAQMIKRSGSFSTVQKGTMAIDMNDQKTKDL", "aa_hexdigest": "fcd1555589bc2af8d9d9776fd99df763", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23061.360500000003, "isoelectric_point": 9.57634334564209 }, "id": "CNOCLNGIAL_888", "locus": "GCA000143945_00873" }, { "type": "cds", "sequence": "contig_1", "start": 489679, "stop": 490461, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTACATTAATAAAGGTGGTGCTTATTCCAGTAACTTCTTAAAGTTTCACCAGTTAGCTTTTAGTGTGGGTTCCAGTTCTGGTTACAATTTTGCTTTTGCTGGTGAAAGCGCTAAACGCTTGGAATTTGGCCAGAAAGCAATCGAGTACCCACGTGACACTTACGAAATTAAGGCACCTACAAATAGCCAAAATGGTAATGGTACCCTTCTAGGTAGTTTTACTAAAAGTAAAAGTAATGGCAAAGAACAAAGTGGGCAAGATGAGGATAACCAGACCAGTGAAACGATTGAGCTTTACAAAAGCAGCGTTCCTAGTGGTAAAGAGGCGGGTAAAAATGCGCTTGCTATTACCAATCAACAACTAATTTCGGCTTTAGAAAATGCTGCAAAAGATAATAAAACCTCCCAACCACAAGCACGATCACTTACAGCTTCTGATCAAGTTCAAATTACACAAAGTTCAGATAAGGTTATTGGTTACATCACCACATCTAATTTGGACATTGACAACAACAATACCTTTGATGTTGGTAAGTTAAACGGTGATAAGTCAACCAGCAAAATCATTGTTAACGCTACTTTAAAAACCTTAAACAAGATCAACACCTTGCAAAGCGAAGAGGGCATTATTCTGCCCCATCCACAAAAATATAAGAGTACTGATCCACAAGCTGTCGCTACCGTTCAGGGTCCAAGTATTATTGGTGTTCATGCTAACGCTAAGGAAAACGCTGAAACCCAAAAATTTATCAACTGGTTTATTAACCAAAAAGAAACTTGA", "aa": "MYINKGGAYSSNFLKFHQLAFSVGSSSGYNFAFAGESAKRLEFGQKAIEYPRDTYEIKAPTNSQNGNGTLLGSFTKSKSNGKEQSGQDEDNQTSETIELYKSSVPSGKEAGKNALAITNQQLISALENAAKDNKTSQPQARSLTASDQVQITQSSDKVIGYITTSNLDIDNNNTFDVGKLNGDKSTSKIIVNATLKTLNKINTLQSEEGIILPHPQKYKSTDPQAVATVQGPSIIGVHANAKENAETQKFINWFINQKET", "aa_hexdigest": "7ae7bafaccd93631191795a44a500021", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28211.81710000002, "isoelectric_point": 7.787539100646972 }, "id": "CNOCLNGIAL_889", "locus": "GCA000143945_00874" }, { "type": "cds", "sequence": "contig_1", "start": 490504, "stop": 490797, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGCAGCACAATACTTTGCTAAGAGCTCTGGTTATGTTTTACCTTACAGTGAAACGTTTACTAAACAAAGTGAAGACGAACACAGCAAAACTAAAGACGCTTATAAGATCTTGAAAGATGTAAACGATGGTAAGTTAGTTGGTTACAGTGATCCAAGTGATTTCCGTTCTGGTAAATTCCGTGACACAATTGTCGCTGCTTTTAGTGGTGCTGTAAGCTCTAAAGCTGACTTTAATAAGTTCTTTAAAGATTTTGAACAACAATTAGGTCAAGAATACAGAAGGGGTTAA", "aa": "MTAAQYFAKSSGYVLPYSETFTKQSEDEHSKTKDAYKILKDVNDGKLVGYSDPSDFRSGKFRDTIVAAFSGAVSSKADFNKFFKDFEQQLGQEYRRG", "aa_hexdigest": "0494f99cd7036ad6b4430385e379a99f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10974.982999999993, "isoelectric_point": 7.886820411682128 }, "id": "CNOCLNGIAL_890", "locus": "GCA000143945_00875" }, { "type": "cds", "sequence": "contig_1", "start": 490771, "stop": 491187, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ], "nt": "GTGGTTTTCGTCACTTTGTCCGTTCTTATCATTCCTTCACCCCGCGCCGGTTCAGAGCAGGAGGTTGGCTTGGTCGAGGGTGGGTCATTTGGAGCCGCCTGTAGTCGTCGTCTGACTGCCACCAAGCGTGGCTTGGACGTTGGCTTTGAGGCCAACGCCGACATTCGAGAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTTAGGCGCGGTTATATCATCCACGCCCCGGTGGTTCTTCCCATACCTGTACCCATTCCCAATCCAGGGCACGTAATCCAACACCTGGTCAAGGAGTGCCCCCGCGAGGTTGAACCCGGTGGAGAACCGGGAAAGGGTGGGGGAGAAGTGGGATTAACCCCTTCTGTATTCTTGACCTAA", "aa": "MVFVTLSVLIIPSPRAGSEQEVGLVEGGSFGAACSRRLTATKRGLDVGFEANADIRERREKGTRTTCVRTSSAGRPRAGFRRGYIIHAPVVLPIPVPIPNPGHVIQHLVKECPREVEPGGEPGKGGGEVGLTPSVFLT", "aa_hexdigest": "dba157ce30a27db0a75f7266c2a55337", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "query_cov": 0.9710144927536232, "subject_cov": 0.950354609929078, "identity": 0.657, "score": 154.0, "evalue": 6.25e-46, "valid": false, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14606.652599999985, "isoelectric_point": 9.635009574890137 }, "id": "CNOCLNGIAL_891", "locus": "GCA000143945_00876" }, { "type": "cds", "sequence": "contig_1", "start": 491977, "stop": 492498, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03257.18" ], "nt": "GTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAACTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTGGTACCACCACCGCAACAGGGGCTGCCTTACCGGAGCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCATTTGATTAAACCTAAGAAAGTTACCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGCGCCAGAATGCTCCAATCATTTGGTACAGAAAATTCCACCCAAGCCCAGCCCCAGTCGCTCAAAACAACGACACCGGTGTTTGGGGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTACCACGCAGGGCTCAAGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACTATTAATAGGAACTTTTCTTATTAA", "aa": "MWDFKGVWADQTHSAVSESQAATSGTTTATGAALPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLIKPKKVTQSDKLDDDLKNLLDPNEVRARMLQSFGTENSTQAQPQSLKTTTPVFGAMSGNLGSVLSGGGTTQGSSSGQSGVDLSPVERVSGSLTINRNFSY", "aa_hexdigest": "4e60f2541ab001f58a0fe9dc731f622d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 116, "aa_cov": 0.6647398843930635, "hmm_cov": 0.9913793103448276, "evalue": 2.6450631034298423e-55, "score": 181.34796142578125, "start": 49, "stop": 163 } ], "seq_stats": { "molecular_weight": 18139.678299999978, "isoelectric_point": 5.85585994720459 }, "id": "CNOCLNGIAL_892", "locus": "GCA000143945_00877" }, { "type": "cds", "sequence": "contig_1", "start": 492584, "stop": 492835, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532788.1", "SO:0001217", "UniParc:UPI0002D77CA5", "UniRef:UniRef100_A0AB38W9C2", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0A0H3DNZ5" ], "nt": "ATGAAATTAGTTAAATTAGCGAGTTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTATTGGCGCTTTTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGTGTTACTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAA", "aa": "MKLVKLASFHRFLKQDQLQLVPLFSPNFLQVLLALFNKPVNLFSENTTMKTNKKYAFAVLLMQAQAKLPNSFKFFLSKKGGMA", "aa_hexdigest": "a0d78bba7fd026ec85ef7e81cbeb0658", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002D77CA5", "ncbi_nrp_id": "WP_017532788.1", "uniref100_id": "UniRef100_A0AB38W9C2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D77CA5", "RefSeq:WP_017532788.1", "UniRef:UniRef100_A0AB38W9C2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W9C2", "uniref90_id": "UniRef90_A0A0H3DNZ5" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DNZ5", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9510.348899999994, "isoelectric_point": 10.471680259704591 }, "id": "CNOCLNGIAL_893", "locus": "GCA000143945_00878" }, { "type": "cds", "sequence": "contig_1", "start": 493003, "stop": 493281, "strand": "+", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_410", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572", "UniRef:UniRef90_P75572" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTATAAAGATTGAAACCACTTTGACCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGTTTGGACAAAATTGATCCACCCAAATAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLIKIETTLTAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPPK", "aa_hexdigest": "f539444a590abb9fb0a5e5098d17bb04", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 492835, "stop": 493281, "observations": { "mutation": [ 492916 ], "stop": [ 492916 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75572", "query_cov": 1.0, "subject_cov": 0.6216216216216216, "identity": 0.935, "score": 105.0, "evalue": 4.96e-27, "gene_start": 1, "gene_end": 92, "reference_start": 57, "reference_end": 148, "reference_sequence": "MKEKTPFKNEKEFHEMVKKTKKGTFSGWYIIDKDNNSVEFSGKFNRQFKLNKPVITVNTEYVTRKELNEYKDSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPSK" }, "paralog": false, "identity": 0.8918918918918919, "score": 201.0, "evalue": 2.86e-67, "description": "Internal stop codon at 492916. Nonsense mutation around 492916.", "psc": { "uniref90_id": "UniRef90_P75572", "product": "UPF0134 protein MPN_410", "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75572", "UniRef:UniRef50_P75572" ] }, "pscc": { "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572" ], "product": "UPF0134 protein MPN_204" } }, "id": "CNOCLNGIAL_894", "locus": "GCA000143945_00879" }, { "type": "cds", "sequence": "contig_1", "start": 493552, "stop": 493845, "strand": "-", "frame": 3, "gene": null, "product": "lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75101", "UniRef:UniRef90_UPI0013300FD7" ], "nt": "ATGGAGCGGAAAGTTGAGTATTTCAATATGGAATATAAAAGCTTTAGTGACTTTTCAGTTAATGCTAGGTTAAGCTATATCTTTAATTGGTATGGGAGTTATTTTGGAGAAAAAAGTTTCACTGCCAATAATGGCGGTAAGCATAATTTCGATCTTTTCCTTAGTATTAAATCTCATTCAAAAAAACAATTTACAGAAAAAAGTTTCATTGTTAAAGATGAAAATTTTCAAGATCAAGATAAAAGTCAACAAATAATTACAGAATGGATTCAGCTTGATCTTTCACTGATTTGA", "aa": "MERKVEYFNMEYKSFSDFSVNARLSYIFNWYGSYFGEKSFTANNGGKHNFDLFLSIKSHSKKQFTEKSFIVKDENFQDQDKSQQIITEWIQLDLSLI", "aa_hexdigest": "3d7eea4762cde666b25b7dc2b6c60885", "start_type": "ATG", "rbs_motif": "AAAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 493600, "stop": 494142, "observations": { "mutation": [ 493935 ], "stop": [ 493935 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013300FD7", "query_cov": 0.8350515463917526, "subject_cov": 0.41968911917098445, "identity": 1.0, "score": 162.0, "evalue": 3.91e-49, "gene_start": 1, "gene_end": 81, "reference_start": 113, "reference_end": 193, "reference_sequence": "MRKKKFLSRFSFSSLFLLCGTLLSACTGIQADLRNLIKETTGKDIDLSKAIKTKEGKKNIITSLKKSYEVNPKDTTKLLLDAWKQSFEEGKLGIAELAFDQATNPTKNSDFKMERKVEYFNMEYKSFSDFSVNARLSYIFNWYGSYFGEKSFTANNGGKHNFDLFLSIKSHSKKQFTEKSFIVKDENFQDQDK" }, "paralog": false, "identity": 0.9222222222222223, "score": 332.0, "evalue": 2e-118, "description": "Internal stop codon at 493935. Nonsense mutation around 493935.", "psc": { "uniref90_id": "UniRef90_UPI0013300FD7", "product": "lipoprotein", "uniref50_id": "UniRef50_P75101", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013300FD7", "UniRef:UniRef50_P75101" ] }, "pscc": { "uniref50_id": "UniRef50_P75101", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75101" ], "product": "Uncharacterized protein MPN_012" } }, "id": "CNOCLNGIAL_895", "locus": "GCA000143945_00880" }, { "type": "cds", "sequence": "contig_1", "start": 493933, "stop": 494181, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGCGCAAAAAGAAATTTTTGTCAAGGTTTGCTTTTGGTTCTTTATTTTTGTTATGTGGAACAATTTTAAGCGCTTGCACTGGTATCCAAGCTGATCTTAGAAATCTTATTAAAGAAGCCACCGGTAAAGACATTGATTTGTCTAAATCAATTAAAACTACAGATGGTAAAAAGAACATAATAACTTCTTCAAAGAAGTCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCATGA", "aa": "MRKKKFLSRFAFGSLFLLCGTILSACTGIQADLRNLIKEATGKDIDLSKSIKTTDGKKNIITSSKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "88fa5813f149f33b93e551c30ce63bfe", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 0.7440000000000001, "score": 116.0, "evalue": 3.37e-32, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "CNOCLNGIAL_896", "locus": "GCA000143945_00881" }, { "type": "cds", "sequence": "contig_1", "start": 494688, "stop": 495140, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321", "UniRef:UniRef50_UPI001375E5B8", "UniRef:UniRef90_Q9EXD5", "UniRef:UniRef90_UPI001375E5B8" ], "nt": "GTGGTGTACTATGAGCAGTTGCAGCGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCAACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGGATGCCCAAAAAGACGACCAGAAGTCCTCGGAATATTTAAGTGGGGAGGCGGGAAGTCAAGCGGGTGGATCCACCCAAGGTACTTCCACCACCACCCAACGTAGGGGTTCGTCAAATGAAAACAAAGTCAAGGTGTTGCAGGTGGCGATGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACCACCTCCAGTTCGGAATCTGGTCAATCAGGTACTCAAGGATCCACCCCAAGTAATCCGGGACCCTGA", "aa": "MVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKDAQKDDQKSSEYLSGEAGSQAGGSTQGTSTTTQRRGSSNENKVKVLQVAMKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTTSSSESGQSGTQGSTPSNPGP", "aa_hexdigest": "a116cbbde6aec246ef7f92eda764ca25", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001375E5B8", "query_cov": 0.86, "subject_cov": 0.8486842105263158, "identity": 0.992, "score": 234.0, "evalue": 4.42e-77, "valid": true, "uniref50_id": "UniRef50_UPI001375E5B8" }, "pscc": { "uniref50_id": "UniRef50_UPI001375E5B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001375E5B8" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 494688, "stop": 495443, "observations": { "mutation": [ 495138, 495300, 495315, 495339 ], "stop": [ 495138, 495300, 495315, 495339 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9EXD5", "query_cov": 0.9, "subject_cov": 0.43973941368078173, "identity": 1.0, "score": 244.0, "evalue": 1.08e-78, "gene_start": 1, "gene_end": 135, "reference_start": 1, "reference_end": 135, "reference_sequence": "MVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKDAQKDDQKSSEYLSGEAGSQAGGSTQGTSTTTQRRGSSNENKVKVLQVAMKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTTSSSESGQSGTQGSTPSNPGPWTPWLTTEQIHNDPAKFAASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFGQALLVFGICLS" }, "paralog": false, "identity": 0.9083665338645418, "score": 449.0, "evalue": 4.92e-162, "description": "Internal stop codon at 495138, 495300, 495315, 495339. Nonsense mutation around 495138, 495300, 495315, 495339.", "psc": { "uniref90_id": "UniRef90_Q9EXD5", "product": "Uncharacterized protein MPN_412", "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9EXD5", "UniRef:UniRef50_P75321" ] }, "pscc": { "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321" ], "product": "Uncharacterized protein MPN_462" } }, "id": "CNOCLNGIAL_897", "locus": "GCA000143945_00882" }, { "type": "cds", "sequence": "contig_1", "start": 495357, "stop": 495611, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAA", "aa": "MLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFGQALLVFGICLS", "aa_hexdigest": "4a15d86ad48e1cca9c8912204bb43c80", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 77, "aa_cov": 0.9166666666666666, "hmm_cov": 0.6623376623376623, "evalue": 2.2471816703023073e-17, "score": 58.75332260131836, "start": 4, "stop": 80 } ], "seq_stats": { "molecular_weight": 9349.227799999997, "isoelectric_point": 4.498829078674317 }, "id": "CNOCLNGIAL_898", "locus": "GCA000143945_00883" }, { "type": "cds", "sequence": "contig_1", "start": 495756, "stop": 496220, "strand": "+", "frame": 3, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_A0AB38W808" ], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGA", "aa": "MGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPKATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLEQTTQ", "aa_hexdigest": "13df9b633592fd302001eeaf1764e1aa", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 495705, "stop": 496392, "observations": { "insertion": [ 496335 ], "mutation": [ 495747, 496218, 496326 ], "stop": [ 495747, 496218, 496326 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W808", "query_cov": 0.974025974025974, "subject_cov": 0.6329113924050633, "identity": 0.987, "score": 284.0, "evalue": 1.45e-95, "gene_start": 1, "gene_end": 150, "reference_start": 17, "reference_end": 166, "reference_sequence": "MRAAHLHTSYELVDWKVGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESGWDNQRSQKNLTNNPGPKAVTGFKLDKGRAYRKLNESWPVSNPSIRPRLARGKVPQNEKRRRQMLRVMLP" }, "paralog": false, "identity": 0.881578947368421, "score": 376.0, "evalue": 3.63e-134, "description": "Internal stop codon at 495747, 496218, 496326. Frameshift due to insertion around 496335. Nonsense mutation around 495747, 496218, 496326.", "psc": { "uniref90_id": "UniRef90_A0AB38W808", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W808", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "CNOCLNGIAL_899", "locus": "GCA000143945_00884" }, { "type": "cds", "sequence": "contig_1", "start": 496431, "stop": 496658, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTTTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGTGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MSSTGSQMAAVTDSQQSGHNSGLVSLAQRSTTVAVQKSDSSGFQGQGTTDNKFQKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "77a80305a02f969b876e134cf9a2a425", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7832.513400000002, "isoelectric_point": 6.697645378112793 }, "id": "CNOCLNGIAL_900", "locus": "GCA000143945_00885" }, { "type": "cds", "sequence": "contig_1", "start": 496917, "stop": 497600, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGAACTTCGGCACTGAAGTTCAAAAAAGCTTCGTTGAAAACCAGTTGGGGTTTAAAGATGACTCCAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGACCTCACCCAACCCGCCTACCTCATCACTGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCGTTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGATCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGA", "aa": "MNFGTEVQKSFVENQLGFKDDSNSDSKNSNLKAQDLTQPAYLITGLDVVQDHLVFAAFKAGAVGYDMTTDSNASTKDQALAWSTTAGLDSAGGYNNLVENTAGLNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLDEARLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVK", "aa_hexdigest": "4a4457435d58dd588483e6fdbdfef737", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.8678414096916299, "subject_cov": 0.8565217391304348, "identity": 0.995, "score": 377.0, "evalue": 6.06e-131, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_901", "locus": "GCA000143945_00886" }, { "type": "cds", "sequence": "contig_1", "start": 497950, "stop": 498405, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAAAAAGAAAAAAACTGCTCCAGGGTTTCTTGAAATTTTTACCGCTTATCATTCCCGCAACCATTTTTGTTAGCTGTGCAAGACGTGAAAGTAATCACCTCATCTTCAACATTTCACTCGATCACGACGCTGATGCTTCTATTAGTAAGTTCTTTGAACTTTACTCTAATAATTTAAGTAAAAAACTAGATAAAAAGGTTACCGTTAGCTTCAACATTATTGATGACTCTTTTACCAAAATTAGCAACATTCAAACTGCTAAAGCTGACTTTGCTTTCGTTAATTCTCAAAGTATTAAAGATAACGGAATAGAAGGATTTAATCTTATTCTTCAAACTCAAACTGATGCATTTAAGGAAGACACCAATTTAGATTATTACAGTGACGGTCAGTTGAAATCTAAAGCAGAAAAAATGACCACTTTATTTTCAAAAACGCCATATAAAGATTGA", "aa": "MLKRKKLLQGFLKFLPLIIPATIFVSCARRESNHLIFNISLDHDADASISKFFELYSNNLSKKLDKKVTVSFNIIDDSFTKISNIQTAKADFAFVNSQSIKDNGIEGFNLILQTQTDAFKEDTNLDYYSDGQLKSKAEKMTTLFSKTPYKD", "aa_hexdigest": "5c58614fea87685bf30c1110ff3049d5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17205.491699999977, "isoelectric_point": 8.887885475158694 }, "id": "CNOCLNGIAL_902", "locus": "GCA000143945_00887" }, { "type": "cds", "sequence": "contig_1", "start": 498565, "stop": 498777, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTATGGCATCGGTCATGGCTCAAGTGGTTCAGCTGGAAAGTTCCAACTACCTGATCTTTTATTAAGAAAGCACTTTGGCTCTAGTTACCCCGGTTTGCAAAATGCAATTAACCAAAACCCTGATAAGTTTGCTAATGTTAGAGGTCGAGAAATAGGTAGAGACAATAAAATCAAAATTGTCTTTGATGATGCTAATTCCTTTGCTTGA", "aa": "MNYGIGHGSSGSAGKFQLPDLLLRKHFGSSYPGLQNAINQNPDKFANVRGREIGRDNKIKIVFDDANSFA", "aa_hexdigest": "469fe2f99e433fdb535c27c8a25b915a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7651.465000000001, "isoelectric_point": 9.598198127746581 }, "id": "CNOCLNGIAL_903", "locus": "GCA000143945_00888" }, { "type": "cds", "sequence": "contig_1", "start": 498901, "stop": 499092, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTATGACATTGGCATTGTAAGTGATACTTTAAGTGATCGCTATCAAAAAGCAATTGCTGAAGTTTTTGTGGAACTAGCTAAAACAAAACAAGATATTTATGGCCCTTCATATGGTTACAACGGTTACAATTTGATTACGGACCCTAACAAAGAAATCTTGGATGTAATTCATAAAACATATGGTTAA", "aa": "MLYDIGIVSDTLSDRYQKAIAEVFVELAKTKQDIYGPSYGYNGYNLITDPNKEILDVIHKTYG", "aa_hexdigest": "d5362b939a7c09d9e9b55ef7033bf097", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7161.999900000001, "isoelectric_point": 4.749375343322753 }, "id": "CNOCLNGIAL_904", "locus": "GCA000143945_00889" }, { "type": "cds", "sequence": "contig_1", "start": 499085, "stop": 499336, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGTTAATCCAGTCTTAGTTTTTGATCAAGTTTCCCTTAAATATAACGGAGCACCACTTTTAGAAAACATTAACTTTACAATTTCACCTGGTGAACACATTTGCTTGTTGGGTAAATCTGGTGTTGGTAAAACTAGTCTTTTAAACTGCATTACTAACACCAAAACAATTTCGAAGGGAACCATTTATTTCAATGGTATTGCTTCTAATAACAAGGATTACAAACAATTAAAAAACAGTTCAGTTTTTTAG", "aa": "MVNPVLVFDQVSLKYNGAPLLENINFTISPGEHICLLGKSGVGKTSLLNCITNTKTISKGTIYFNGIASNNKDYKQLKNSSVF", "aa_hexdigest": "1cd040ac91abe6ef9721fdb784f5a0a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 59, "aa_cov": 0.7108433734939759, "hmm_cov": 1.0, "evalue": 1.0685504510234686e-14, "score": 50.724910736083984, "start": 21, "stop": 79 } ], "seq_stats": { "molecular_weight": 9023.326699999992, "isoelectric_point": 9.264961051940915 }, "id": "CNOCLNGIAL_905", "locus": "GCA000143945_00890" }, { "type": "cds", "sequence": "contig_1", "start": 499432, "stop": 499818, "strand": "+", "frame": 1, "gene": "p29", "product": "putative ABC transporter ATP-binding protein p29", "db_xrefs": [ "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47532", "UniRef:UniRef90_P75370" ], "nt": "GTGGAACCTCAATCGCTAAGAGAACAAATTATCCAAATCCTTGAAGAGGTTAACCTAAAGGAATATGTCACTTACATTATTAAGGACTTGTCAGGTGGTCAAAAACAACGTGTGGAAGTAGCTAAATTGTTCTTTGCCAATTCCCAAGTTTTATTAGTTGATGAGCCCACTACTGGACTTGATTTAATCAATGCGCACAAAATCATGGAACTAATTACCCAATTTGCGCGCCAAAAAGCAATGACCTTAATCTTTGTGACCCATGATGTTGAGTTTGCGCTCAAATACAGTGACCGTATTATTGCTCTAAAAAATAAGGCATTAGTTTTAGATCAAGCTACAAATAAGCTTACCAAACAAAAGTTAATGCAAATTTATCATGACTAG", "aa": "MEPQSLREQIIQILEEVNLKEYVTYIIKDLSGGQKQRVEVAKLFFANSQVLLVDEPTTGLDLINAHKIMELITQFARQKAMTLIFVTHDVEFALKYSDRIIALKNKALVLDQATNKLTKQKLMQIYHD", "aa_hexdigest": "35b12e31d8b5d7238a7513f96e01fa03", "start_type": "GTG", "rbs_motif": null, "genes": [ "p29" ], "truncated": "5-prime", "pseudogene": { "start": 499133, "stop": 499818, "observations": { "deletion": [ 499316 ], "stop": [ 499385, 499415 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75370", "query_cov": 1.0, "subject_cov": 0.5245901639344263, "identity": 0.9840000000000001, "score": 239.0, "evalue": 2.81e-78, "gene_start": 1, "gene_end": 128, "reference_start": 117, "reference_end": 244, "reference_sequence": "MVNPVLVFDQVSLRYNGAPLLENINFTISPGEHICLLGKSGVGKTSLLNCITNTKTISKGTIYFNGIASNNKDYKQLKKQFSFLDQVPNLIDTDFVYDAIWREAKNNLKWWQRLFLVEPQSLREQIIQILEEVNLKEYVTYIIKDLSGGQKQRVEVAKLFFANSQVLLVDEPTTGLDLINAHKIMELIIQFARQKAMTLIFVTHDVEFALKYSDRIIALKNKALVLDQATNKLTKQKLMQIYHD" }, "paralog": false, "identity": 0.9868421052631579, "score": 433.0, "evalue": 7.67e-157, "description": "Internal stop codon at 499385, 499415. Frameshift due to deletion around 499316.", "psc": { "uniref90_id": "UniRef90_P75370", "gene": "p29", "product": "Probable ABC transporter ATP-binding protein p29", "uniref50_id": "UniRef50_P47532", "cog_id": "COG1120", "cog_category": "PH", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75370", "UniRef:UniRef50_P47532", "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47532" ], "product": "Probable ABC transporter ATP-binding protein p29" } }, "id": "CNOCLNGIAL_906", "locus": "GCA000143945_00891" }, { "type": "cds", "sequence": "contig_1", "start": 500015, "stop": 500257, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCTCTTACCTACTGCAAACCATTTTTTATGTCGTGAGTGGTAGTATTTTGGGATTTGTCATAGCCCTGTGGTTTTCGTACCTCACTGCCTTTAAAATCCAGCCACTTTACATCGCTTTACCAACTCGCTTATTCACCATTTTTCTGCGTTCCTTTCCAGTACTTGTGTTTGCTTTTTTGTTTAACAATTTGTTTAACAAACAACTAACAGCAACCTTAACGATTACCTGGTTTAGTTGA", "aa": "MGSYLLQTIFYVVSGSILGFVIALWFSYLTAFKIQPLYIALPTRLFTIFLRSFPVLVFAFLFNNLFNKQLTATLTITWFS", "aa_hexdigest": "3275dc4062964590309d04ffad3c665b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9256.969499999988, "isoelectric_point": 9.994614219665529 }, "id": "CNOCLNGIAL_907", "locus": "GCA000143945_00892" }, { "type": "cds", "sequence": "contig_1", "start": 500444, "stop": 500662, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTATTGCTGGCATTACTGGAATTGGTGAGCTCATTGCCACACCTCTAGGGGGCACAGTGCAGCTTAACTTAGTTTTAATACCGATGTTAACTTTGATCGGCTTTTTACTGTTTTTAGAAGCGAGTGTTTTTCTGTTAACTAAGTTTGTTTTACAAAAACAGAGCCAAGCCGGTGATTACTTTTTACAAGCTAAAACACTGCAAAAGAGAAAGTGA", "aa": "MSIAGITGIGELIATPLGGTVQLNLVLIPMLTLIGFLLFLEASVFLLTKFVLQKQSQAGDYFLQAKTLQKRK", "aa_hexdigest": "f988381b71b2440f54ca07680c3befa8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7822.357799999995, "isoelectric_point": 9.69799518585205 }, "id": "CNOCLNGIAL_908", "locus": "GCA000143945_00893" }, { "type": "cds", "sequence": "contig_1", "start": 500672, "stop": 501175, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTACATCCTAGCTTTGGTTTTAGCGGCTTTTACCTTGGCTAATTTAGTACAGTTGGACTATACGGTTAAAGCGCCAGGGTTTGTGGCTGACTTTTTTAAACAGTTCTTTCAAACAAAAACAGCTTTTTTAATTAGTGAAGACGCTAACATTAACCCACTTTTAATGTTGTTAAAACTCACAACTCAAGCGATTAGTTTAATTACCTTAGTGTTTGTGTTAGCACTCTTGTTTGGCTTTTTAGCATCAAAGCTGTTTTCCACTATAACCTCAATTAGCTTAAAACTGTTACTTTTAGTGATTAGGGTAATTCCCAGTGTCTTACTGTTCCGCTTATTTGATCCAATTATTTTTCGCCCAGAAACTACCATTATCTTTGTTTTAGCAATCCACAGTGCTGCTAGTTATGGTCAACTGATAACGATTAACTTTGATAATGCCAACGAAGGGGTAATTAACAACATGCAGAACCACGGCTTTAGTAGGTTTTACATCTTGTGA", "aa": "MIYILALVLAAFTLANLVQLDYTVKAPGFVADFFKQFFQTKTAFLISEDANINPLLMLLKLTTQAISLITLVFVLALLFGFLASKLFSTITSISLKLLLLVIRVIPSVLLFRLFDPIIFRPETTIIFVLAIHSAASYGQLITINFDNANEGVINNMQNHGFSRFYIL", "aa_hexdigest": "af800cc8c3311ccb8242ea5532686aef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18696.141499999987, "isoelectric_point": 9.048734092712401 }, "id": "CNOCLNGIAL_909", "locus": "GCA000143945_00894" }, { "type": "cds", "sequence": "contig_1", "start": 501257, "stop": 501421, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGGGATCTTTGGTGGTTCGATTATTGGGGTGCGAATTAACAATTTCTTTGAGCGAGCACAGTACTCTGAATTGGGCACAATAACCTTACCCTTAATGGTTTACTTAATGGTGTTTGAAGTCATTTTAATGGCAGTACGGTTAAACAAACTCAAAGTGTGA", "aa": "MFGIFGGSIIGVRINNFFERAQYSELGTITLPLMVYLMVFEVILMAVRLNKLKV", "aa_hexdigest": "1eed773931340b1c0185abbaed8f2722", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6154.420099999999, "isoelectric_point": 9.518902015686034 }, "id": "CNOCLNGIAL_910", "locus": "GCA000143945_00895" }, { "type": "cds", "sequence": "contig_1", "start": 501440, "stop": 501862, "strand": "-", "frame": 2, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "COG:COG0816", "COG:JLK", "EC:3.1.-.-", "EC:3.1.21.10", "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364", "RefSeq:WP_014325523.1", "SO:0001217", "UniParc:UPI0001DD63E0", "UniRef:UniRef100_A0AB33HMQ4", "UniRef:UniRef50_Q9ZB76", "UniRef:UniRef90_P57114" ], "nt": "GTGCAGTACATTTTAGGCATTGATTTTGGTTTGAAGAGAATTGGTACAGCACTAGCCAATACCATTGACCGCTTTCCAAGTCCCTTCCGCGTGTTTGCGGTACAAAATAATTTGCAACAAGCCGTTAATACCCTTTTTAAGGATTTAAAGCAAGCTGGTTATGAATTAGTGCAAATAGTGATTGGCTTTCCCCACTTCCACTACCAGTCAAGTATCCAAGTGAGCATTCACAAGTTTGTGGAACTAATAAAAACCCGCTTTAATGTACCAGTAACACTCATTGATGAAAGTGGTACTACGAGTGAGGTAAAAGCTAACTTGCAGGAATTAGGCTTAAAAAACAGAACCTTTAAAAAGGCCAAGGACACCTTAGCGGCTACCTTAATCTTAGAGCGTTTCTTAAACCAACAAAAACCCAATTAA", "aa": "MQYILGIDFGLKRIGTALANTIDRFPSPFRVFAVQNNLQQAVNTLFKDLKQAGYELVQIVIGFPHFHYQSSIQVSIHKFVELIKTRFNVPVTLIDESGTTSEVKANLQELGLKNRTFKKAKDTLAATLILERFLNQQKPN", "aa_hexdigest": "fd720376636ad9189676bc3c1a3257ed", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63E0", "ncbi_nrp_id": "WP_014325523.1", "uniref100_id": "UniRef100_A0AB33HMQ4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63E0", "RefSeq:WP_014325523.1", "UniRef:UniRef100_A0AB33HMQ4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HMQ4", "uniref90_id": "UniRef90_P57114" }, "psc": { "uniref90_id": "UniRef90_P57114", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.-.-", "3.1.21.10" ], "uniref50_id": "UniRef50_Q9ZB76", "cog_id": "COG0816", "cog_category": "JLK", "go_ids": [ "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB76" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "CNOCLNGIAL_911", "locus": "GCA000143945_00896" }, { "type": "cds", "sequence": "contig_1", "start": 501862, "stop": 502350, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACAAGCAAAAAACTGCTTTAACTAAGCAACTAAATCACTTTCACTTGCCGCAAGTAATTACTGATTTACGTAAATGCCAAGCTTTACTAAATGAACTCAAAATAACGGTTAATGAACTGAAAACGGAAGACTTTAAGTGGAAGCAAAAGCAGTTAGCGGAAAAGATCAAACAGGAATTGTTAGAGCTAGCAAAGCAAGACAAAGCTTATGTTCTCGCTAGCTTTGCAGCAGTTGATCCGAAACTGTTATCACAAGTAGCCCAAGCAGTCTTGAACCAACACAAAAACAAGCTGTTTGTACTCCTCAACCAATTCAACAACAGTCCGTCCTTTATGTTATTAGGGCAAGATGTTTCTAAATGTATTCAGCTTTTAAAAGCTCACTTTGAACTTAAGGGTGGGGGTAGTAATAACTTCTTTAGGGGTTCGTTTAACGAGTCGGTGGATGTAAGTAAACTACAAGCTATCCTCGATACCCTTCAATAG", "aa": "MNKQKTALTKQLNHFHLPQVITDLRKCQALLNELKITVNELKTEDFKWKQKQLAEKIKQELLELAKQDKAYVLASFAAVDPKLLSQVAQAVLNQHKNKLFVLLNQFNNSPSFMLLGQDVSKCIQLLKAHFELKGGGSNNFFRGSFNESVDVSKLQAILDTLQ", "aa_hexdigest": "858125cfe8d0ecc94c0f72852f2ab4f4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18422.22019999999, "isoelectric_point": 9.439734840393069 }, "id": "CNOCLNGIAL_912", "locus": "GCA000143945_00897" }, { "type": "cds", "sequence": "contig_1", "start": 502471, "stop": 502722, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "GTGGAACACAGGATTCGACAAATTATTCAACAAGACATTCAGCGCGAAGAGATCTTTACTGATCTAGAGGGTAGTCAAAAACTTAATGCGATTGCCTACTTTGAAGAGGAGTATTCGAACCACGAGTTGTTACGGGTAATTCGCTTTGGTGATTTTAGTGTCGAGCTTTGTGGTGGTACCCATGTGGAACACACTGGTTTAATTGAGAACTGTTTTATTACTGATTACTATGCTCGTGGCACAGGGCGTTGA", "aa": "MEHRIRQIIQQDIQREEIFTDLEGSQKLNAIAYFEEEYSNHELLRVIRFGDFSVELCGGTHVEHTGLIENCFITDYYARGTGR", "aa_hexdigest": "906ac5d966814cf2bbcd1d4b368bde07", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 37, "aa_cov": 0.4457831325301205, "hmm_cov": 1.0, "evalue": 5.041727944835668e-14, "score": 47.535125732421875, "start": 44, "stop": 80 } ], "seq_stats": { "molecular_weight": 9751.746899999991, "isoelectric_point": 4.915629386901855 }, "id": "CNOCLNGIAL_913", "locus": "GCA000143945_00898" }, { "type": "cds", "sequence": "contig_1", "start": 502738, "stop": 503283, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAGCACCGTTTGATCTCCAAACAAAACAGTAATACGTTGAGCTTTGAAAAGCAAAATGAACACTTAGTGAACTTTAAAACAGCGAGTGAGTTCTTATACGAGGCTAATGAGATCACCGCCAAGGTCATTGGGCTGTTTGATGAACAGTACCAACCGGTGCAAAAACTGCACAACCAAAGTGGTTATGTGGTGTTTGACCAAACGGTGCTGTATGCCACATCAGGCGGTCAGCGTTATGATGAGGGGTACTGTATTAACCACAGCCAAAATGATCAAAGAGTAAGCTTTCAGGGCGTGTTTAAAGGCCCTAATAAACAACACTTCCACTTCTTTTTAACTGGTAGTTTTCAACTAGGTGACAAAGTGATCCTAGTACATGACGGTAAGTGGCGCCAACTAGTGAAAAACAACCACAGTTTAGAACACTTACTGCACGCGGCATTGCAAAAGGAAATTGACCCCTTGATTAAACAAGACGGCGCCTTTAAGTCAGCGCAAAAAGCTACCATTGACTTTAATTTTTCGCGAGCCTTAACCTGA", "aa": "MEQHRLISKQNSNTLSFEKQNEHLVNFKTASEFLYEANEITAKVIGLFDEQYQPVQKLHNQSGYVVFDQTVLYATSGGQRYDEGYCINHSQNDQRVSFQGVFKGPNKQHFHFFLTGSFQLGDKVILVHDGKWRQLVKNNHSLEHLLHAALQKEIDPLIKQDGAFKSAQKATIDFNFSRALT", "aa_hexdigest": "b183fc40ff4877e61fecd2dd9fcfc333", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20830.082300000013, "isoelectric_point": 7.165941810607911 }, "id": "CNOCLNGIAL_914", "locus": "GCA000143945_00899" }, { "type": "cds", "sequence": "contig_1", "start": 503302, "stop": 503433, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTTGAAAAGAGTATTGCTCATAATGCACTCAAACCCCAAGTAGCCTTCCAGTTGTACGAAACCTATGGCTTTCCGATTGAAATGATTAAGGAACTGGTGGATAAGCGCCAACTCCAGGTAGACTGA", "aa": "MLFEKSIAHNALKPQVAFQLYETYGFPIEMIKELVDKRQLQVD", "aa_hexdigest": "ea8e8dd49d402ee4e2404e79108d020f", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5082.889900000002, "isoelectric_point": 5.648738670349121 }, "id": "CNOCLNGIAL_915", "locus": "GCA000143945_00900" }, { "type": "cds", "sequence": "contig_1", "start": 503476, "stop": 503973, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAGTGTGGAACATTGTCTTTAGTCAGTTTAACAATGATGGCAATGGTAACTACAGTGAGTTAGCGCAGAAGAACATCGATACTGGGGCCGGTATTGAACGCCTAGTAGCAATCTTACAGGACGCGCCGACTAACTTTGACACGGATATCTTCTTAAAGTTAATTGGCATTATTGAACAGCACTGTAAGCATAAGTATGATACCAACTTATACTTTAAGTTTGACCAAAAGTTAAACGAAGCGCAAAGTGCCTTTCGCATTATTAGTGACCACTTTAAGGCAATTACCTTCACCATTGCCGAAGGCGTATTGCCAGGACCCAACGAGCGCAGTTACATTGTGCGCCGGTTACTGCGCCGCGCTTTGTTGGCCTGCAAAAAACTGGACTTAGACTTAAAGTTTATTGATCCGATGGTCGATGCCATTATTAGCGTTTATGGGTCTTACTACCAGCAGTTGCAGGGGAAAAACCAGGTGGTGCAACAAGCAATCTGA", "aa": "MEVWNIVFSQFNNDGNGNYSELAQKNIDTGAGIERLVAILQDAPTNFDTDIFLKLIGIIEQHCKHKYDTNLYFKFDQKLNEAQSAFRIISDHFKAITFTIAEGVLPGPNERSYIVRRLLRRALLACKKLDLDLKFIDPMVDAIISVYGSYYQQLQGKNQVVQQAI", "aa_hexdigest": "b6913b4e3ad5fad4030215ddddc32e9e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18876.393899999977, "isoelectric_point": 6.283743858337403 }, "id": "CNOCLNGIAL_916", "locus": "GCA000143945_00901" }, { "type": "cds", "sequence": "contig_1", "start": 504154, "stop": 504300, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGGTAACTTCTCGATTGGTGATTACTTTAAGGAAGAGGCCATTGGCTTTGCCAATGATCTTTTAGTCAATCATTACCACTTGGATCCGAAGCGCTTTTACATTACAGTGTACCAAGATGATGAATTGACCTTTAACACCTGA", "aa": "MLGNFSIGDYFKEEAIGFANDLLVNHYHLDPKRFYITVYQDDELTFNT", "aa_hexdigest": "d61c1cab050927d79af6d19310353653", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5676.2373, "isoelectric_point": 4.444434165954591 }, "id": "CNOCLNGIAL_917", "locus": "GCA000143945_00902" }, { "type": "cds", "sequence": "contig_1", "start": 504835, "stop": 505341, "strand": "-", "frame": 3, "gene": "ugpQ", "product": "putative MG293-like protein", "db_xrefs": [ "COG:COG0584", "COG:I", "GO:0006629", "GO:0008081", "SO:0001217", "UniRef:UniRef50_P75367", "UniRef:UniRef90_P75367" ], "nt": "ATGCTTAAACGACAACTTCTGCTAGCACACCGTGGGTACAGTGATATTGCGCCTGAAAACACCCAGCTAGCGTTTGAATTAGCGTTCCAATATCGCTTTGATGGCGTGGAGTTAGATGTGCACTTGACCAAGGACGGGGAGTTAGTGATTATCCATGATGAAACTACCACCAGAACAGCGCTAGTCGACAAAACAATCGAACTGGAAACACTCGCTAGTTTAAAGCAAGATGATCACAGTGCTTTCTTTAAGTTCAAAACGCAACCCCAGCCGATTATGACCTTAAAGGAGTTCTTTGATCAGTACTTAGATAAATTTCAACTAATTAACGTGGAAATTAAAACTGACCAAAAGGAATACCCGGGAATTGAAGCTAAAATTGATGCTTTGGCCCAACAGTATGGCAAAAAAGTAATTGAAAAGGTAGTGTTCTCCTCGTTTAACTTTGCTTCCTTACAGCGCTTGTATGACATTAACCCCAACTACCAAATAGCCTTTTTGTTTTGA", "aa": "MLKRQLLLAHRGYSDIAPENTQLAFELAFQYRFDGVELDVHLTKDGELVIIHDETTTRTALVDKTIELETLASLKQDDHSAFFKFKTQPQPIMTLKEFFDQYLDKFQLINVEIKTDQKEYPGIEAKIDALAQQYGKKVIEKVVFSSFNFASLQRLYDINPNYQIAFLF", "aa_hexdigest": "9150a4b1420c7ec40b6892eac91394d1", "start_type": "ATG", "rbs_motif": null, "genes": [ "ugpQ" ], "truncated": "5-prime", "pseudogene": { "start": 504616, "stop": 505341, "observations": { "mutation": [ 504705, 504768, 504837 ], "stop": [ 504705, 504768, 504837 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75367", "query_cov": 1.0, "subject_cov": 0.6970954356846473, "identity": 1.0, "score": 330.0, "evalue": 2.23e-113, "gene_start": 1, "gene_end": 168, "reference_start": 1, "reference_end": 168, "reference_sequence": "MLKRQLLLAHRGYSDIAPENTQLAFELAFQYRFDGVELDVHLTKDGELVIIHDETTTRTALVDKTIELETLASLKQDDHSAFFKFKTQPQPIMTLKEFFDQYLDKFQLINVEIKTDQKEYPGIEAKIDALAQQYGKKVIEKVVFSSFNFASLQRLYDINPNYQIAFLFWTKKQFQAVDALKIKQVCQYLHPWTNIYEKFPDMVLSLQLPLGLWTLNSEVKFHQFRQDRMVYAQIANKKFEV" }, "paralog": false, "identity": 0.9875518672199171, "score": 468.0, "evalue": 4.47e-170, "description": "Internal stop codon at 504705, 504768, 504837. Nonsense mutation around 504705, 504768, 504837.", "psc": { "uniref90_id": "UniRef90_P75367", "gene": "UgpQ", "product": "Uncharacterized protein MG293 homolog", "uniref50_id": "UniRef50_P75367", "cog_id": "COG0584", "cog_category": "I", "go_ids": [ "GO:0008081", "GO:0006629" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75367", "UniRef:UniRef50_P75367", "COG:COG0584", "COG:I", "GO:0008081", "GO:0006629" ] }, "pscc": { "uniref50_id": "UniRef50_P75367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75367" ], "product": "Uncharacterized protein MG293 homolog" } }, "id": "CNOCLNGIAL_918", "locus": "GCA000143945_00903" }, { "type": "cds", "sequence": "contig_1", "start": 505391, "stop": 505981, "strand": "-", "frame": 2, "gene": null, "product": "putative MG294-like protein", "db_xrefs": [ "GO:0022857", "KEGG:K26602", "SO:0001217", "UniRef:UniRef50_P75366", "UniRef:UniRef90_P75366" ], "nt": "ATGGGAATGTATGTCTTTCAAAGTACGTTTGCTTATTATCTTTTACAAATGATGCAAAACGCCTTTTTGGCACCAGTGATCTTAGGGACTGTACTGGGTGGTGTAAGGACGTATGTTTTGCGCAGTGCTGTAAGTGTGTACTTGGGCAGGTTAGCTGATAAGTTTAGGAGCTACATCCTCTTTTTAATGTTGTGTACAGGACTTGGCATTATCTTTGTCTTGATGTTTATTCTCTTAGGTTTTGGGCAAGTGGGCCAACAGCAAAACTATGCCTTGATTATCGTATCGGCGATCCTCTACATTCTCACCGGGGTGTTGTCTTGGGGTATGGTAACCGTTAGGTACAACCAGGTAGCGGAAATAGAGATTGGTAAAAACAACTATGCTTCTAGTGTTGGTTTACTTTCCTTTATTGGTTTTTCGACTGATGGTTGGCTCTACACGGTTACAAGTGTCGTGGGTAAGGCTTATACCCCAGACGGTCAGAAGAATACCTCGATTCAGGGTTACCAAATTATTGCAGCTATTTGTCTAGGAATAGCCCTGTTTGGTTTATTGTGTGGTACGATTGTCTTTTTGGTTAATACTTGA", "aa": "MGMYVFQSTFAYYLLQMMQNAFLAPVILGTVLGGVRTYVLRSAVSVYLGRLADKFRSYILFLMLCTGLGIIFVLMFILLGFGQVGQQQNYALIIVSAILYILTGVLSWGMVTVRYNQVAEIEIGKNNYASSVGLLSFIGFSTDGWLYTVTSVVGKAYTPDGQKNTSIQGYQIIAAICLGIALFGLLCGTIVFLVNT", "aa_hexdigest": "d292932f2a7c8b84e976e4ff46349281", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 505334, "stop": 506281, "observations": { "mutation": [ 505393, 506002 ], "stop": [ 505393, 506002 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75366", "query_cov": 1.0, "subject_cov": 0.4126315789473684, "identity": 1.0, "score": 367.0, "evalue": 3.04e-124, "gene_start": 1, "gene_end": 196, "reference_start": 261, "reference_end": 456, "reference_sequence": "METKLSLKKRLQQFSKKQLIALIILGAADVFVIAAPYYIKNVVPNLHLYLGITEDEVATLTSIIGYVTLATQLPGGFLANRFSSRKLLFLSEISTGVITFWLATNILTRESQKSNALFVQYCVIWGLWGITSTLIFWTPLWKLASQQATQENQALGFGIQGAANGVWGFIFIFLIALIITAVAYPAGGESSANNPAPFAIYAFIIGGMLLVTGFTVLFFVPEKPIEKYDSHTSLKTAKKNFEQILITLKNWKLWLLSFFLMGMYVFQSTFAYYLLQMMQNAFLAPVILGTVLGGVRTYVLRSAVSVYLGRLADKFRSYILFLMLCTGLGIIFVLMFILLGFGQVGQQQNYALIIVSAILYILTGVLSWGMVTVRYNQVAEIEIGKNNYASSVGLLSFIGFSTDGWLYTVTSVVGKAYTPDGQKNTSIQGYQIIAAICLGIALFGLLCGTIVFLVNTWELKRLGKTDYRWRTLDNA" }, "paralog": false, "identity": 0.9936507936507937, "score": 615.0, "evalue": 6.87e-224, "description": "Internal stop codon at 505393, 506002. Nonsense mutation around 505393, 506002.", "psc": { "uniref90_id": "UniRef90_P75366", "product": "Uncharacterized protein MG294 homolog", "uniref50_id": "UniRef50_P75366", "kegg_orthology_id": "K26602", "go_ids": [ "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75366", "UniRef:UniRef50_P75366", "KEGG:K26602", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P75366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75366" ], "product": "Uncharacterized protein MG294 homolog" } }, "id": "CNOCLNGIAL_919", "locus": "GCA000143945_00904" }, { "type": "cds", "sequence": "contig_1", "start": 506000, "stop": 506299, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTTGGTATTCAGGGCGCTGCTAATGGTGTTTGGGGTTTTATTTTTATTTTCTTAATTGCCTTGATTATTACAGCAGTAGCTTACCCAGCCGGTGGTGAAAGTAGTGCTAATAATCCCGCACCGTTTGCCATTTATGCCTTTATTATTGGTGGCATGTTGCTCGTTACTGGGTTTACCGTGCTGTTCTTTGTACCAGAAAAACCAATTGAAAAGTACGACAGTCATACCAGTTTAAAAACTGCCAAAAAGAACTTTGAACAGATTCTCATTACCTTAAAGAACTGGAAGTTGTGA", "aa": "MGFGIQGAANGVWGFIFIFLIALIITAVAYPAGGESSANNPAPFAIYAFIIGGMLLVTGFTVLFFVPEKPIEKYDSHTSLKTAKKNFEQILITLKNWKL", "aa_hexdigest": "63a585f44eef0f019808b1faa2201401", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10771.587999999987, "isoelectric_point": 9.050474739074708 }, "id": "CNOCLNGIAL_920", "locus": "GCA000143945_00905" }, { "type": "cds", "sequence": "contig_1", "start": 506351, "stop": 506761, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACGAAACTTAGTTTAAAGAAACGGTTGCAGCAGTTTTCCAAAAAGCAGCTCATAGCACTAATTATTCTCGGTGCGGCTGATGTTTTTGTAATTGCGGCCCCGTACTATATTAAGAATGTGGTGCCTAACTTGCACCTGTATTTAGGGATTACTGAAGATGAAGTGGCCACCTTAACCTCGATTATTGGTTATGTGACCTTAGCCACGCAGTTACCTGGTGGTTTTTTAGCGAACCGGTTTAGTTCGCGGAAACTATTGTTCTTGTCAGAAATTTCAACGGGTGTAATTACCTTTTGGCTAGCTACTAACATTCTTACTCGTGAAAGCCAAAAATCCAATGCCTTGTTTGTACAGTACTGTGTTATTTGGGGGCTTTGGGGTATTACTTCGACCTTAATCTTTTGA", "aa": "METKLSLKKRLQQFSKKQLIALIILGAADVFVIAAPYYIKNVVPNLHLYLGITEDEVATLTSIIGYVTLATQLPGGFLANRFSSRKLLFLSEISTGVITFWLATNILTRESQKSNALFVQYCVIWGLWGITSTLIF", "aa_hexdigest": "1e1de476bf12f7f72e44b0cc1490fdea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15201.783599999988, "isoelectric_point": 9.518063926696776 }, "id": "CNOCLNGIAL_921", "locus": "GCA000143945_00906" }, { "type": "cds", "sequence": "contig_1", "start": 506892, "stop": 507173, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20258.4" ], "nt": "TTGTATGTCTCGAGTGACCCAGAAGATTTACAGCGTCGTGGCATTACCCTTTCCCACTTTAACTGGTTGTACCAACCCAATCCATTGACCCAAACCGTTCTGGTACGGATTCGCCATGCCCAACCGTTAGTGCAAGGGCACATAACAGTACAACCAAACAACGTCGTGCAAGTACAACTAGATCAACCAATTGACCGCGTTACCAACGGTCAGTATGGTGTTTTGTATACCCAGAATGGCATTTGTTTGGGGAGTGGTATCATTACCGCTAGCCAAATCTAA", "aa": "MYVSSDPEDLQRRGITLSHFNWLYQPNPLTQTVLVRIRHAQPLVQGHITVQPNNVVQVQLDQPIDRVTNGQYGVLYTQNGICLGSGIITASQI", "aa_hexdigest": "dce2dc16aa520893f5e25662d3119ee3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20258.4", "name": "tRNA_Me_trans_C", "length": 77, "aa_cov": 0.8172043010752689, "hmm_cov": 0.987012987012987, "evalue": 2.542333641956583e-25, "score": 84.11976623535156, "start": 13, "stop": 88 } ], "seq_stats": { "molecular_weight": 10424.715799999996, "isoelectric_point": 6.807856178283691 }, "id": "CNOCLNGIAL_922", "locus": "GCA000143945_00907" }, { "type": "cds", "sequence": "contig_1", "start": 507303, "stop": 507677, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03054.22" ], "nt": "ATGTTGTGTAACCGTTTAATTAAATTTGGGCTAATGCGTGATTACTGCAAGCAGTTAGATCCCAACAGTGACTTTGCTACAGGGCATTATGCGGCACTTAGCTGGGACAACAACCAACCTTTACTGGCCATTCCCAAAGACAAGCACAAGGACCAAACCTATTTTTTAGCGCATGTCAAACCAGCGCAACTACAAGACGTGGTGTTTCCCTTGGCGCATTTACTGAAAACGGAGGTGCGGCAAATAGCGTTAGCACACCACTTTAGTGTGGCTACTAAAAAGGACTCAACCGGCATTTGCTTTATTGGGGAACGCCACTTTAGTGACTTCTTAAAGAATTATTTACCAGTCAAACCGGGGGTTATTTATGATTGA", "aa": "MLCNRLIKFGLMRDYCKQLDPNSDFATGHYAALSWDNNQPLLAIPKDKHKDQTYFLAHVKPAQLQDVVFPLAHLLKTEVRQIALAHHFSVATKKDSTGICFIGERHFSDFLKNYLPVKPGVIYD", "aa_hexdigest": "ba9d228296723ef3e820f3960e70ff9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 106, "aa_cov": 0.8387096774193549, "hmm_cov": 0.9905660377358491, "evalue": 1.50309190378185e-38, "score": 127.94937133789062, "start": 1, "stop": 104 } ], "seq_stats": { "molecular_weight": 14199.280199999983, "isoelectric_point": 8.776741981506348 }, "id": "CNOCLNGIAL_923", "locus": "GCA000143945_00908" }, { "type": "cds", "sequence": "contig_1", "start": 507738, "stop": 508004, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGCAAAATAAAACCGTTTTTGTCGGAATATCAGGTGGAGTGGACTCAGCTGTGAGTGCGCTCCTTTTAAAGCAACAGTACCGCGAAGTGATTGGCATTTTTATGGAGTGCTGGGACAATACACTAAATAACGACCAGTTAGGTCACCGCGCCTTTAACGAGCATAAGTCGGGTTGTTCGTCCAAAGAAGATTTTCGCGAAGCGCAAGCAATTGCCCAATTACTAGGAATCAAACTAATTAAGCAAAACCTGGTGGAACCGTACTGA", "aa": "MQNKTVFVGISGGVDSAVSALLLKQQYREVIGIFMECWDNTLNNDQLGHRAFNEHKSGCSSKEDFREAQAIAQLLGIKLIKQNLVEPY", "aa_hexdigest": "1932614864dae796562766c5f9c6253f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 82, "aa_cov": 0.9318181818181818, "hmm_cov": 0.7804878048780488, "evalue": 6.356285779724542e-08, "score": 27.460763931274414, "start": 3, "stop": 84 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 85, "aa_cov": 0.9659090909090909, "hmm_cov": 0.8941176470588236, "evalue": 2.0030206383215875e-22, "score": 75.30052185058594, "start": 4, "stop": 88 } ], "seq_stats": { "molecular_weight": 9856.130999999994, "isoelectric_point": 6.052977561950684 }, "id": "CNOCLNGIAL_924", "locus": "GCA000143945_00909" }, { "type": "cds", "sequence": "contig_1", "start": 508005, "stop": 508394, "strand": "-", "frame": 1, "gene": null, "product": "putative MG296-like protein", "db_xrefs": [ "RefSeq:WP_010874779.1", "SO:0001217", "UniParc:UPI00001395B3", "UniRef:UniRef100_P75364", "UniRef:UniRef50_P75364", "UniRef:UniRef90_P75364" ], "nt": "ATGAAACCACAACTTTTAGCCTTGAAGCAGTTTGTGCAAACTGAGTTTGAAAAAGTTGATTTTGAGACCTTTCGCCAAAACTTTAATCGTTGTTTGGAACGCGAACAGAGTACCCTGTTAATTTATGAAGATGATGATTATGATGATCAGTCCTTCTTTTTAAAACCGATGCTCAGTGACGCCTTTTTTATTAGTAGTGAAGTGGTCAAACAACTGGATTTGTTAGCTGTTTTAGTGGACAATCCTAAAGGTGATGTCAAAAGTTGTTGTCAGAGCTTTTATGAAGCGTTAACCTTATTTATTTCAGCGCTGGCCATAACTAAGGGTGTTGATGTCGGGCGTTACCACCAGCAGTTGGGTAAGCGCTTTGGTGTTTTAACAGTTTACTAG", "aa": "MKPQLLALKQFVQTEFEKVDFETFRQNFNRCLEREQSTLLIYEDDDYDDQSFFLKPMLSDAFFISSEVVKQLDLLAVLVDNPKGDVKSCCQSFYEALTLFISALAITKGVDVGRYHQQLGKRFGVLTVY", "aa_hexdigest": "bd500a8f610e597845d6216cb1c1a641", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001395B3", "ncbi_nrp_id": "WP_010874779.1", "uniref100_id": "UniRef100_P75364", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001395B3", "RefSeq:WP_010874779.1", "UniRef:UniRef100_P75364" ] }, "ips": { "uniref100_id": "UniRef100_P75364", "uniref90_id": "UniRef90_P75364" }, "psc": { "uniref90_id": "UniRef90_P75364", "product": "Uncharacterized protein MG296 homolog", "uniref50_id": "UniRef50_P75364" }, "pscc": { "uniref50_id": "UniRef50_P75364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75364" ], "product": "Uncharacterized protein MG296 homolog" }, "genes": [], "id": "CNOCLNGIAL_925", "locus": "GCA000143945_00910" }, { "type": "cds", "sequence": "contig_1", "start": 508394, "stop": 508702, "strand": "-", "frame": 2, "gene": "ylxM", "product": "YlxM family DNA-binding protein", "db_xrefs": [ "COG:COG2739", "COG:K", "GO:0048500", "KEGG:K09787", "RefSeq:WP_010874780.1", "SO:0001217", "UniParc:UPI000013A7F1", "UniRef:UniRef100_P75363", "UniRef:UniRef50_P75363", "UniRef:UniRef90_P75363" ], "nt": "ATGAACCAAACCAAACTTAACCAGCGTTTAAAGCAACAGCAACTATTTGATATTTACGGGGAACTTTTAACCAAACGGCAAGCGTGTTACTTTAACGAGTACATTAACCTGGATTTGAGCATGCAAGAGATTGCTGATAAATACCAAGTTAAGAAAAGTTCAATCCACCAACACATCAAAACCTGCAACCTGATCTTTAACCGTTTTGAAGCGAAATTGCAGTTGTTTAAAAAGCAACAGCTCCGTTTAAAGTTGTACGAAAAAATAACAGATCCCCAATTACGCGAGCAACTTATTAAACTAAGGTAA", "aa": "MNQTKLNQRLKQQQLFDIYGELLTKRQACYFNEYINLDLSMQEIADKYQVKKSSIHQHIKTCNLIFNRFEAKLQLFKKQQLRLKLYEKITDPQLREQLIKLR", "aa_hexdigest": "65aa099fec66c2c2366037bd6aea5dbc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A7F1", "ncbi_nrp_id": "WP_010874780.1", "uniref100_id": "UniRef100_P75363", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A7F1", "RefSeq:WP_010874780.1", "UniRef:UniRef100_P75363" ] }, "ips": { "uniref100_id": "UniRef100_P75363", "uniref90_id": "UniRef90_P75363" }, "psc": { "uniref90_id": "UniRef90_P75363", "gene": "ylxM", "product": "YlxM family DNA-binding protein", "uniref50_id": "UniRef50_P75363", "cog_id": "COG2739", "cog_category": "K", "kegg_orthology_id": "K09787", "go_ids": [ "GO:0048500" ] }, "pscc": { "uniref50_id": "UniRef50_P75363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75363" ], "product": "UPF0122 protein MPN_424" }, "genes": [ "ylxM" ], "id": "CNOCLNGIAL_926", "locus": "GCA000143945_00911" }, { "type": "cds", "sequence": "contig_1", "start": 508683, "stop": 509114, "strand": "-", "frame": 1, "gene": null, "product": "Cell division protein FTSY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "nt": "GTGATCTTCCGTGGGGTGCAACAAGGCATTCAAAACGAATACGACTTTGTGTTGTGTGACACCTCGGGACGGTTGCAAAACAAAACCAACTTAATGAATGAGTTGAAAAAGATTTACCAAATTGTCCAGAAGGTGAGTAGCGCTAAACCCCAAGAAACACTGCTCGTTTTAGATGGTACTACCGGACAATCGGGGTTAGCACAGGCCAAGGTCTTTAATGAATTTACCGAGCTTACCGGGATTATCCTTACCAAAATGGACAGCTCCTCTAAGGGTGGGATTATCCTAGCGATTAAAGACCTGTTTAACTTACCGGTAAAACTGATTGGTTTTGGAGAAACAACTGCTGATTTAGCGGCCTTTGATTTAGAGCAATATGTACTGGGTCTTACCAAGAATTTAAGCCTCAACCATGAACCAAACCAAACTTAA", "aa": "MIFRGVQQGIQNEYDFVLCDTSGRLQNKTNLMNELKKIYQIVQKVSSAKPQETLLVLDGTTGQSGLAQAKVFNEFTELTGIILTKMDSSSKGGIILAIKDLFNLPVKLIGFGETTADLAAFDLEQYVLGLTKNLSLNHEPNQT", "aa_hexdigest": "7ec3940ce35358f300c5d2fe3e475f24", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PYR8", "query_cov": 0.8601398601398601, "subject_cov": 0.968503937007874, "identity": 0.675, "score": 169.0, "evalue": 1.15e-51, "valid": false, "product": "Cell division protein FTSY", "uniref50_id": "UniRef50_A0A3B0PYR8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PYR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "product": "Cell division protein FTSY" }, "genes": [], "id": "CNOCLNGIAL_927", "locus": "GCA000143945_00912" }, { "type": "cds", "sequence": "contig_1", "start": 509172, "stop": 509729, "strand": "-", "frame": 1, "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "db_xrefs": [ "COG:COG0552", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006614", "GO:0009306", "GO:0016887", "KEGG:K03110", "SO:0001217", "UniRef:UniRef50_P75362", "UniRef:UniRef90_P75362" ], "nt": "ATGAGTTTTATCCACAAGCTAATCCAGAAGTTTAAACCGAAAAAGAAACTGGTCGACCAAGTGCAACAAGCTGTCCAGGAAAAGAGCTTCTTTCAAGCCAACCAAAAGAGCTATTACCAGGGTTTAAACAAGTCGGCTAACAGCTTTGCCAATACCATTAATAAACTAGCAGCTAACTATGTAACGGTTAACGAACAGTTTCAAGAAAGTTTGTTTGAGGAGTTAGTGTTGCTTGATATTGGTTACCATGCGGCTACTAAAATCTGTGATGCGATTGTCCAAGAACTCAAACTACAACGAGTGAGTGATCCTCAGTTAATCCAGGAAATTATTGTCGATAAGTTAATTGTTTACTATATCCAAGATAAGTTGTTTGAAACTGATTTAACGGTTGAAGCTAATAAAACCAACGTGTATTTGTTTGTCGGGGTTAATGGTGTTGGCAAAACAACTTCATTAGCCAAACTAGCTGACCAGCTTACCAAGCAAAACAAACGGGTGTTAATGGTCGCTGGGGATACCTTTCGCGCTGGGGCAGTGGCCCAGTTAGCAGAATGA", "aa": "MSFIHKLIQKFKPKKKLVDQVQQAVQEKSFFQANQKSYYQGLNKSANSFANTINKLAANYVTVNEQFQESLFEELVLLDIGYHAATKICDAIVQELKLQRVSDPQLIQEIIVDKLIVYYIQDKLFETDLTVEANKTNVYLFVGVNGVGKTTSLAKLADQLTKQNKRVLMVAGDTFRAGAVAQLAE", "aa_hexdigest": "72e09c2c86b35f85ac3ac3bab2b294cf", "start_type": "ATG", "rbs_motif": null, "genes": [ "ftsY" ], "truncated": "5-prime", "pseudogene": { "start": 508869, "stop": 509729, "observations": { "mutation": [ 509174 ], "stop": [ 509174 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75362", "query_cov": 1.0, "subject_cov": 0.5316091954022989, "identity": 1.0, "score": 345.0, "evalue": 2.83e-117, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MSFIHKLIQKFKPKKKLVDQVQQAVQEKSFFQANQKSYYQGLNKSANSFANTINKLAANYVTVNEQFQESLFEELVLLDIGYHAATKICDAIVQELKLQRVSDPQLIQEIIVDKLIVYYIQDKLFETDLTVEANKTNVYLFVGVNGVGKTTSLAKLADQLTKQNKRVLMVAGDTFRAGAVAQLAEWAQRIGCDIVLPNPKEETPAVIFRGVQQGIQNEYDFVLCDTSGRLQNKTNLMNELKKIYQIVQKVSSAKPQETLLVLDGTTGQSGLAQAKVFNEFTELTGIILTKMDSSSKGGIILAIKDLFNLPVKLIGFGETTADLAAFDLEQYVLGLTKNLSLNHEPNQT" }, "paralog": false, "identity": 0.9965034965034965, "score": 551.0, "evalue": 5.69e-201, "description": "Internal stop codon at 509174. Nonsense mutation around 509174.", "psc": { "uniref90_id": "UniRef90_P75362", "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P75362", "cog_id": "COG0552", "cog_category": "U", "kegg_orthology_id": "K03110", "go_ids": [ "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006614", "GO:0009306", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75362", "EC:3.6.5.4", "UniRef:UniRef50_P75362", "COG:COG0552", "COG:U", "KEGG:K03110", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006614", "GO:0009306", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75362", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75362" ], "product": "Signal recognition particle receptor FtsY" } }, "id": "CNOCLNGIAL_928", "locus": "GCA000143945_00913" }, { "type": "cds", "sequence": "contig_1", "start": 509735, "stop": 512230, "strand": "-", "frame": 2, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261", "KEGG:K03529", "SO:0001217", "UniRef:UniRef50_P75361", "UniRef:UniRef90_P75361" ], "nt": "GTGGAAGCCAAACCAGAGGAGCGACGTAAGATCTTTGAGGACGCTTCGGGGATTGGGCGGTACACTAAGCGCAAAGAAGAGGTTACCAACCAATTAGCGCGTACCGTGCAAAATCTCAAACAGGTCAGCATTGTTTTAAATGAACTCAAAAAGGACTTAAAGAAGCTTACCATTCAAGCGGACAAGGCCCAGCGCTTTGTCAAACTTAAGGAAGAATTAAAGGAGCTGGAGCTGAGTGTCTTGGTCGCAGATTACCTCAAGTCCCAGGGTGAGTTAGACCGCTTTAACCACCAAATTGGTTACATCGAACAGGACTTTAAACTACACGAACCGCAGTTACAACTGCTAGAAGACCAGCTCAACATCTTTAACCAGCGCTTTCGTGATGCTGATGAACAGTCCATCAAACTACAACAAGAACTGCAAGCGGTCTACCAAACGATTAACGAGTTGGAACAACGCAAGGCCGTGATTGATGTGCAGCTCAAAAACGAGTTGTCCAAAAAGGACGAAAAGCACAAAATCCAGGCGTTAAAAAAGTTAATCCGGGTAGACCAAGCGCAACTAGAAAGCTTACAAGCGCAAGTACTCAAAACAACCAGTGAAATTACCCTGTTAACCAATGAGTTGAGCACCGTCCAAACTGAGTTGGACACCACAAAGTTGAATCTTAACCAAAACAGTGCGGCGTTAGTCTACCAACAAGCGCAGCAAGAGTTTTTAAAAGCGCAAAACGAGGAGTGGGTCAAAACGAATCCGGCCCATGTTTTGGTCAAAAATGTCAAGGCCCTCACCGGTCTCTTAAACACGCTCAACACTTTTTTAAAGTTTGAAAAGCAGTACGAAAAGGCCTTGTTAAAGGCGTTGGGTAAATCAATTGGTTATCTGGTTGTTAACAACAACTTGGCGGCTTTAAAGGCAATTGATTTTTTGTTAACTAACCAGATTGGTCAAGTTACCTTTTTACCAATTGACGACATTGCCTTTGATACTAAGATAGTTCCCGAGCACATGGAAATTTTGCAACAACTCGATGGCTTTTTGGGTGTTGGTAGTGACCATGTGAGTTGTGATGAAAGGTTACAACCAATTGTTAATGCTTTATTGGGTCAAGTAATTATTGCCAGTGACCTTCAAGCCGCTTTAAAACTGTCCAGTTATACCTATAAGCTGTACCGCGTTGTCACCCTCAACGGGGAAACGGTGTACGCTGGAGGGATTATCCAGGGTGGTTATGTCAAGGACAACTTAAGCTTGTATAACCTCCAGGAAAAGTTAGCTTCTAGTGAAGCTAACATTACCCAACTAGAACATAACGAAAAGCAACTACGTACCAATCTCACTAGCTTAGAGACTAAACTCAACGAGCTCAACAAAAAGTTAAAGTACGAAGAAATCCTACTGGAAAAGTTTAACGAACGTGTTAACCACACCAATAAGGCAATTTTGAGCTATAAGATTGAGTATGAGCAGCTCACCAACGAAAGCTTTGACGGAACCCCGCACAGCTTTGATGAAACCAGATTGGTTGAATCATTAAACCGTGCTTGGGCTGAACGTGACGAACTCAATTCACAGTTAAAGCTCAACCAGGAATTGAAAGAAACACTGGCCAAATCGATTAAACTAGCCGAAGCCAAAACCGCTGACCTCCGTGCCCTTTTAGATGAACAACGCTCCCAGTTAGTGTTGGCACGCGAAGGCAAAATCCGCTTTGAAAACACAATCCACAACATTACCGACAAGATTAACGGTGGGTATAAACTAACGATGGAGTTTGCGATCGCTAACTATAACAAACCAATTAAGCTCTCCACCATGCAAGCGCAAAACAAGATTGCCAGGATGCAGAGCCAACTCGATGAAATGGGTCCAATTAACCTGGAGTCGATTGCTGAAATTGCTGATAAGCAAAAGCGCTTTGATGACATTAACGGTGAGTATGAATCACTGCAAACGGCGATTAAGGATTTGCAAACCGCGATTGGTGAAATTGATGAATTGGCCTGCAAGGAATTTGACGAGCTCATCCAAAAAGTTAATGCAGAACTACCTAAAACCTTTAACTACCTCTTTGGCGGTGGTTCGTGTCAAATTCGCTACACGGATACTGATAACGTCTTGTTGTCAGGCATAGAAGTGTTTGCTAATCCGCCTGGCAAAAATGTCGCTAACTTAATGTTGTTATCGGGTGGGGAGAAAACACTCGTGGCCTTGTCGGTCCTCTTTAGTATTTTGAGAGTTAGTGCCTTTCCTCTAGTAATTTTAGATGAAGCGGAGAGTGCCTTAGATCCGGCGAACGTGGAGCGCTTTGCTAACATTATTGGCAATTCCAGTAACAACACCCAGTTCTTAATTATTACCCACCGTCAGGGCACGATGATGAAGTGTGACATGTTACTGGGAGCGGCAATGCAGACCAAGGGAGTGACCAAGACGTTTGCCGTTTCTTTGGAAAAAGCAGAGCAGTACATTAGTAAAGATAAGCAGAACTAA", "aa": "MEAKPEERRKIFEDASGIGRYTKRKEEVTNQLARTVQNLKQVSIVLNELKKDLKKLTIQADKAQRFVKLKEELKELELSVLVADYLKSQGELDRFNHQIGYIEQDFKLHEPQLQLLEDQLNIFNQRFRDADEQSIKLQQELQAVYQTINELEQRKAVIDVQLKNELSKKDEKHKIQALKKLIRVDQAQLESLQAQVLKTTSEITLLTNELSTVQTELDTTKLNLNQNSAALVYQQAQQEFLKAQNEEWVKTNPAHVLVKNVKALTGLLNTLNTFLKFEKQYEKALLKALGKSIGYLVVNNNLAALKAIDFLLTNQIGQVTFLPIDDIAFDTKIVPEHMEILQQLDGFLGVGSDHVSCDERLQPIVNALLGQVIIASDLQAALKLSSYTYKLYRVVTLNGETVYAGGIIQGGYVKDNLSLYNLQEKLASSEANITQLEHNEKQLRTNLTSLETKLNELNKKLKYEEILLEKFNERVNHTNKAILSYKIEYEQLTNESFDGTPHSFDETRLVESLNRAWAERDELNSQLKLNQELKETLAKSIKLAEAKTADLRALLDEQRSQLVLAREGKIRFENTIHNITDKINGGYKLTMEFAIANYNKPIKLSTMQAQNKIARMQSQLDEMGPINLESIAEIADKQKRFDDINGEYESLQTAIKDLQTAIGEIDELACKEFDELIQKVNAELPKTFNYLFGGGSCQIRYTDTDNVLLSGIEVFANPPGKNVANLMLLSGGEKTLVALSVLFSILRVSAFPLVILDEAESALDPANVERFANIIGNSSNNTQFLIITHRQGTMMKCDMLLGAAMQTKGVTKTFAVSLEKAEQYISKDKQN", "aa_hexdigest": "fd607c419f245cd178faf83aa0539c22", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75361", "query_cov": 1.0, "subject_cov": 0.8462321792260692, "identity": 0.996, "score": 1433.0, "evalue": 0.0, "valid": true, "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_P75361", "cog_id": "COG1196", "cog_category": "D", "kegg_orthology_id": "K03529", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_P75361", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75361" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "CNOCLNGIAL_929", "locus": "GCA000143945_00914" }, { "type": "cds", "sequence": "contig_1", "start": 512234, "stop": 512683, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13175.12", "PFAM:PF13304.12", "PFAM:PF13476.12", "PFAM:PF13555.12", "PFAM:PF02463.25" ], "nt": "ATGGTTTTTCTCAAACGGTTTCGTGCGTATGGTTTTAAGTCATATGCTGACGAAATAACAATTAATTTTACCCACTCAATGACCGGTATAGTCGGTCCTAATGGTTCTGGCAAGTCCAACGTTGTCGATGCTTTGAAGTGGGTCTTAGGGGAACGCAGCATGAAGCACTTGCGCAGTAAGTCCGGTGATGATATGATCTTCTTTGGTTCTAAGGACAAGCCCGCTAGTAAGCTAGCGGAAGTGGAACTGACCTTTGACAATTCCCAAAAACTACTGCACGATCCCCGACCAGAGATCTCGGTGATGCGCCGGATCTATCGTGGTAGTGGTCAGAGTGAGTACTATATTAACGGCGAGTTAGTAACGCTAAAAGAAATTAGTGGGATCTTTGCTGATATTGGTTTGGAAAAAGGCTCGTTGGGGATTATTTCCCAGGGCAGTGTGTCGTGA", "aa": "MVFLKRFRAYGFKSYADEITINFTHSMTGIVGPNGSGKSNVVDALKWVLGERSMKHLRSKSGDDMIFFGSKDKPASKLAEVELTFDNSQKLLHDPRPEISVMRRIYRGSGQSEYYINGELVTLKEISGIFADIGLEKGSLGIISQGSVS", "aa_hexdigest": "a809eed79516bc538aaff6562854ba4f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13175.12", "name": "AAA_15", "length": 65, "aa_cov": 0.4228187919463087, "hmm_cov": 0.7692307692307693, "evalue": 1.0154208553249806e-10, "score": 37.22410202026367, "start": 11, "stop": 73 }, { "id": "PF13304.12", "name": "AAA_21", "length": 67, "aa_cov": 0.3221476510067114, "hmm_cov": 1.0, "evalue": 1.9725977969953166e-09, "score": 33.38786315917969, "start": 27, "stop": 74 }, { "id": "PF13476.12", "name": "AAA_23", "length": 124, "aa_cov": 0.8053691275167785, "hmm_cov": 0.9354838709677419, "evalue": 6.680046904180926e-18, "score": 61.50141525268555, "start": 11, "stop": 130 }, { "id": "PF13555.12", "name": "AAA_29", "length": 37, "aa_cov": 0.24161073825503357, "hmm_cov": 0.972972972972973, "evalue": 4.0866758242008384e-08, "score": 28.39857292175293, "start": 14, "stop": 49 }, { "id": "PF02463.25", "name": "SMC_N", "length": 137, "aa_cov": 0.912751677852349, "hmm_cov": 0.9854014598540146, "evalue": 8.009438067042218e-43, "score": 141.84580993652344, "start": 3, "stop": 138 } ], "seq_stats": { "molecular_weight": 16498.679799999987, "isoelectric_point": 9.124742317199708 }, "id": "CNOCLNGIAL_930", "locus": "GCA000143945_00915" }, { "type": "cds", "sequence": "contig_1", "start": 512868, "stop": 513740, "strand": "+", "frame": 3, "gene": "cof", "product": "Putative phosphatase MPN_427", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "RefSeq:WP_014325526.1", "SO:0001217", "UniParc:UPI0001DD63E3", "UniRef:UniRef100_A0AAX0S2J5", "UniRef:UniRef50_P75360", "UniRef:UniRef90_P75360" ], "nt": "ATGACAAAGACAAGTAAAGCATCCGGGTTAAGCTGGTTTTTTTGTGATTTAGATGGCACTTTACTGCGTTACCAAAACAACCAGCACTTAATTGAACCAACTACGAAGCGAGCAGTGGCACAGTTAGTGGAGAGTGGTGCCAACTTTGTTGTAGCTACTGGACGCAAACCGAGTGACGTGCGCAACATTTACAAGGAGTTGGGGATTGAACAAGCCAGTCCCTACTTAATTGCCAACAACGGCGCGGTAGTGTGGGATTTAAAGCGCAATAGCTACCTCAACAAGCAAACCTTGTCTTTAAGTGACTTTGACTTAATTGACCACATTAACCAAACGTTAAACCAATTAAACCACGAGTATGGCTGCATTCTGTATGGCCTCAACGACCAGGTGTACTTTTATCATATCCACGCTCCTGACTCACAGGCCTTCAAACAGTACTTTGCTTTTTATGAAGGCGAATTTGTCCAAAACCAGTATCTGGAAATAGATGGGCTCAAAACTGAATACAATCTAGTTAAAGCCATTTGGTTTTTTAAGGAAGTGCACCAGCAAAAGGCGGTGATTGCGCAACACTTTACTAACCAAGAACGCTTGGTCATTACTTCAGCACACTCGTTTGAACTAGTACCGCTTAATGTATCAAAGGGTCATGCGATAAACCTAATTAAACAACAAGTAAAAATCACCGATAACCAGATTATGGTCCTCGGTGACTCTTACAATGACTTGCCAATGTTTCAACATGGTGTAGTTAAGGTAACTAATCACCTCGCACCCGACAACCTCAAACAACTAGCTACAAGGGTTTATGACCTACCAGCTTCACTGTTTGTGGGTCAAGCGTTGAACGACTACTTTAAGTTCGATTAA", "aa": "MTKTSKASGLSWFFCDLDGTLLRYQNNQHLIEPTTKRAVAQLVESGANFVVATGRKPSDVRNIYKELGIEQASPYLIANNGAVVWDLKRNSYLNKQTLSLSDFDLIDHINQTLNQLNHEYGCILYGLNDQVYFYHIHAPDSQAFKQYFAFYEGEFVQNQYLEIDGLKTEYNLVKAIWFFKEVHQQKAVIAQHFTNQERLVITSAHSFELVPLNVSKGHAINLIKQQVKITDNQIMVLGDSYNDLPMFQHGVVKVTNHLAPDNLKQLATRVYDLPASLFVGQALNDYFKFD", "aa_hexdigest": "ea889d1df022db654c8c8fbf827c0aa8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63E3", "ncbi_nrp_id": "WP_014325526.1", "uniref100_id": "UniRef100_A0AAX0S2J5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63E3", "RefSeq:WP_014325526.1", "UniRef:UniRef100_A0AAX0S2J5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S2J5", "uniref90_id": "UniRef90_P75360" }, "psc": { "uniref90_id": "UniRef90_P75360", "gene": "Cof", "product": "Putative phosphatase MPN_427", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P75360", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P75360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75360" ], "product": "Putative phosphatase MPN_427" }, "genes": [ "cof" ], "id": "CNOCLNGIAL_931", "locus": "GCA000143945_00916" }, { "type": "cds", "sequence": "contig_1", "start": 513746, "stop": 514618, "strand": "-", "frame": 2, "gene": "pta", "product": "phosphate acetyltransferase", "db_xrefs": [ "COG:C", "COG:COG0280", "EC:2.3.1.8", "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959", "SO:0001217", "UniRef:UniRef50_P47541", "UniRef:UniRef90_P75359" ], "nt": "ATGGAAGCAGTTAACCAGCTCCAGCAAGCGGGTATTTTAACCCCAGCGGTGATCTTCCGCACCCGCAGTGAAGTACCAGCTGGTTTTAACACCGCCATCAAGCACTATGTGATTGAGGAAATGGACTTGACAAAGTACGCTAACTTCGTTTATGAAAAGCGCAAGCACAAGGGCATGGAAATGCGTGAAGCACAAAAGTTTGTCCGTGATGCCAGTTCCCTAGCAGCCACTTTAGTAGCACTCAACGAAGTGGATGGGGAAGTGTGTGGTAAGGAATACGCTACGAAGGATACTCTCCGTCCTGCTTTACAACTGTTAGGTACTGGTAACTTTGTGTCGAGTGTGTTCATCATGGAAAAGAATGAGGAACGCTTGTACTTTACCGACTGTGCTTTTGCTGTTTACCCTTCACCCCAAGAATTAGCAGTTGTAGCGGAAAACACCTTTAAGTTTGCCCAAAGTATGGGGGAACCAGAACTCAAGATGGTCTTTTTGAGCTATTCAACACTGGGCAGTGGTAAAGGGGAAGCGGTGGACAAAGTAGTCAGTGCGACCCAAATCTTCTTGGAAAAACACCCCGAACTCAAAGCTAATGTTTGTGGTGAATTACAGTTTGACAGTGCTTTTGTGGAAAAGGTACGCAAACAAAAAGCCCCTAATTTGACTTGGAATGGATCAGCTAACATCTATGTGTTCCCGAACCTAGATGCTGGTAACATTGGATACAAGATTGCGCAACGTTTAGGTGGTTATGAAGCAATTGGTCCAATTGTTTTAGGTCTAGCACGTCCTTTTAACGACCTTTCCCGTGGTGCCAGTGTCAGTGATGTCTTTAACGTGGGCATTATTACCGCAGCGCAAACACTTAAATAA", "aa": "MEAVNQLQQAGILTPAVIFRTRSEVPAGFNTAIKHYVIEEMDLTKYANFVYEKRKHKGMEMREAQKFVRDASSLAATLVALNEVDGEVCGKEYATKDTLRPALQLLGTGNFVSSVFIMEKNEERLYFTDCAFAVYPSPQELAVVAENTFKFAQSMGEPELKMVFLSYSTLGSGKGEAVDKVVSATQIFLEKHPELKANVCGELQFDSAFVEKVRKQKAPNLTWNGSANIYVFPNLDAGNIGYKIAQRLGGYEAIGPIVLGLARPFNDLSRGASVSDVFNVGIITAAQTLK", "aa_hexdigest": "151464f33bb12b05af6bc4ab739144ff", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75359", "query_cov": 1.0, "subject_cov": 0.90625, "identity": 0.997, "score": 570.0, "evalue": 9.08e-205, "valid": true, "gene": "pta", "product": "phosphate acetyltransferase", "ec_ids": [ "2.3.1.8" ], "uniref50_id": "UniRef50_P47541", "cog_id": "COG0280", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959" ] }, "pscc": { "uniref50_id": "UniRef50_P47541", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47541" ], "product": "Phosphate acetyltransferase" }, "genes": [ "pta" ], "id": "CNOCLNGIAL_932", "locus": "GCA000143945_00917" }, { "type": "cds", "sequence": "contig_1", "start": 514746, "stop": 515060, "strand": "-", "frame": 1, "gene": null, "product": "phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021F8CEA1" ], "nt": "ATGTCCTTGGATGTGGGTAGCAAAACAGTGGCCCTGTTTAGCAGTTACCTCGCCAAGGCCAAAACAATCTTTTGGAATGGTCCCATGGGCGTATTTGAATTTAGCAACTATGCTCAGGGTACCTTAGCAATTGGTAAAGCGATTGCGCAAAACCAACAAGCCTTTAGTGTCATTGGCGGTGGTGATTCTGCAGCCGCTGCTAAACAACTCCAGATTGCTGATCAATTTAGTTTTATTTCCACTGGTGGTGGTGCATCTTTAGCACTAATAGGTGGGGAAGAGCTAGTGGGAATTAGCGATATTCAAAAAAAGTAA", "aa": "MSLDVGSKTVALFSSYLAKAKTIFWNGPMGVFEFSNYAQGTLAIGKAIAQNQQAFSVIGGGDSAAAAKQLQIADQFSFISTGGGASLALIGGEELVGISDIQKK", "aa_hexdigest": "583382ee057a7fee52f78e5db83ec487", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021F8CEA1", "query_cov": 0.8269230769230769, "subject_cov": 0.8958333333333334, "identity": 0.605, "score": 105.0, "evalue": 8.99e-28, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_UPI0021F8CEA1", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_UPI0021F8CEA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021F8CEA1" ], "product": "phosphoglycerate kinase" }, "genes": [], "id": "CNOCLNGIAL_933", "locus": "GCA000143945_00918" }, { "type": "cds", "sequence": "contig_1", "start": 515082, "stop": 515528, "strand": "-", "frame": 1, "gene": null, "product": "phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000851E545" ], "nt": "TTGGGTGACATCTTTGTCAATGACGCCTTTGGCACCGCCCACCGCCGTCACGCTTCCAACGCTGGCATTGCCAAGTACATCAAAAACTCCTGCATTGGTCTTTTAATGGAGCGGGAATTGATCAACCTCTACCGCTTAATTAATAACCCACCCAAACCGTTTGTGGTTGTGTTGGGTGGCGCTAAGGTTTCTGATAAGTTACAGGTAGTAAATAACCTGCTCAAGATTGCGGATCATATTTTAATAGGTGGGGGCATGATCAACACCTTCCTCAAAGCTCTGGGTAACGAAGTTGGCACTTCCTTAGTGGAGGAAGACTTAGTCCAAACCGCCAAGCAAATCCTTGACAGTGACAAGGACAAGAAAATTGTCTTGGGGGTAGACCAAATGCTTCATGCTAGCTTTAAGGATGAACCAGGCGTGGAGTGTGTTGTAGCCCCCCAATGA", "aa": "MGDIFVNDAFGTAHRRHASNAGIAKYIKNSCIGLLMERELINLYRLINNPPKPFVVVLGGAKVSDKLQVVNNLLKIADHILIGGGMINTFLKALGNEVGTSLVEEDLVQTAKQILDSDKDKKIVLGVDQMLHASFKDEPGVECVVAPQ", "aa_hexdigest": "987d60df65782bf94adf80841a8886cd", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00210B3D86", "query_cov": 0.9054054054054054, "subject_cov": 1.0, "identity": 0.5, "score": 133.0, "evalue": 1.65e-37, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_UPI000851E545", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_UPI000851E545", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000851E545" ], "product": "phosphoglycerate kinase" }, "genes": [], "id": "CNOCLNGIAL_934", "locus": "GCA000143945_00919" }, { "type": "cds", "sequence": "contig_1", "start": 515535, "stop": 515975, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGGTTGACTTTAAAACAGTCCAAGCGTTTGACTTTCAGGGTAAAACCGTAGTCTTACGTGCTGACCTCAATGTGCCGATGAAGGACGGGGTCATTACCGACAACGAACGCATACTAGCTAGCTTAGACACGATTAAGTACCTCTTGGGTCACAATTGCAAAATTGTGTTACTGTCCCATTTGTCACGAGTCAAATCGCTCGATGACAAAAAGGGTAAGAAGTCACTCCAACCAGTAGCTAGTGCTTTACAAAATTTATTAAAGGACACCAAGGTTCACTTTTGTCCGGAAAACACGGGTGACAAGGTCAAAGTCGCAGTCAATCAACTCCCATTAGGGGAAATTTTGGTGTTGGAAAACACGCGTTACTGTGATGTGAATGAGGCTGGTGAAGTGGTTAAACACGAAAGCAAGAATAACGCTGAGTTAGCACAGTTCTGA", "aa": "MVDFKTVQAFDFQGKTVVLRADLNVPMKDGVITDNERILASLDTIKYLLGHNCKIVLLSHLSRVKSLDDKKGKKSLQPVASALQNLLKDTKVHFCPENTGDKVKVAVNQLPLGEILVLENTRYCDVNEAGEVVKHESKNNAELAQF", "aa_hexdigest": "5192ccd40eec708169cefd0dfc7f8ec9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 137, "aa_cov": 0.9315068493150684, "hmm_cov": 0.9051094890510949, "evalue": 1.313135770370323e-36, "score": 121.81835174560547, "start": 8, "stop": 143 } ], "seq_stats": { "molecular_weight": 16230.581599999983, "isoelectric_point": 7.714608573913575 }, "id": "CNOCLNGIAL_935", "locus": "GCA000143945_00920" }, { "type": "cds", "sequence": "contig_1", "start": 515978, "stop": 516700, "strand": "-", "frame": 2, "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "COG:COG0057", "COG:G", "EC:1.2.1.12", "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0016020", "GO:0016620", "GO:0031639", "GO:0034394", "GO:0050661", "GO:0051287", "SO:0001217", "UniRef:UniRef50_P47543", "UniRef:UniRef90_P75358" ], "nt": "GTGGTTGAATCCACTGGTCGCTTTGTTAGTGAAGAAGGTGCTAGTTTGCACTTGCAAGCTGGTGCTAAACGCGTGATTATTTCCGCTCCAGCTAAACAAAAAACAATTAAAACCGTGGTTTACAACGTGAACCACAAGATTATTAACGCTGAGGACAAAATTATCTCAGCTGCTAGCTGTACCACCAATTGCTTAGCACCAATGGTACACGTCTTGGAAAAGAACTTCGGCATCTTACACGGTACCATGGTAACGGTACACGCTTACACCGCCGACCAACGTTTACAAGACGCACCCCACAGCGATCTTCGTCGTGCCCGTGCTGCTGCTTGCAACATTGTACCTACCACCACTGGAGCTGCCAAAGCAATTGGCCTAGTAGTGCCAGAAGCTACTGGTAAGCTCAACGGTATGGCCCTTCGTGTTCCCGTACTAACTGGTTCGATCGTTGAACTTTGTGTAGCCCTTGAAAAGGATGCTACCGTAGAACAAATTAACCAAGCAATGAAGAAGGCGGCTTCCGCTTCCTTTAGGTACTGTGAGGATGAAATTGTGTCCAGTGACATTGTCGGCTCGGAACACGGTTCCATCTTTGACTCCAAGCTAACCAACATTATTGAAGTGGATGGTAACAAGCTTTACAAGGTTTACGCATGGTATGACAACGAATCCTCTTACGTAAACCAGTTGGTACGGGTAGTTAACTATTGTGCCAAGCTTTAA", "aa": "MVESTGRFVSEEGASLHLQAGAKRVIISAPAKQKTIKTVVYNVNHKIINAEDKIISAASCTTNCLAPMVHVLEKNFGILHGTMVTVHAYTADQRLQDAPHSDLRRARAAACNIVPTTTGAAKAIGLVVPEATGKLNGMALRVPVLTGSIVELCVALEKDATVEQINQAMKKAASASFRYCEDEIVSSDIVGSEHGSIFDSKLTNIIEVDGNKLYKVYAWYDNESSYVNQLVRVVNYCAKL", "aa_hexdigest": "49f813cf80852db2683f96dfcbf204c1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0012BB6B15", "query_cov": 0.9958333333333333, "subject_cov": 0.9409448818897638, "identity": 0.544, "score": 230.0, "evalue": 1.84e-72, "valid": false, "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A960BYG6", "go_ids": [ "GO:0006006", "GO:0016620", "GO:0050661", "GO:0051287" ] }, "genes": [ "gap" ], "truncated": "3-prime", "pseudogene": { "start": 515978, "stop": 516991, "observations": { "mutation": [ 516724 ], "stop": [ 516724 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75358", "query_cov": 1.0, "subject_cov": 0.712166172106825, "identity": 0.996, "score": 419.0, "evalue": 7.96e-146, "gene_start": 1, "gene_end": 240, "reference_start": 98, "reference_end": 337, "reference_sequence": "MLAKSKTIRVAINGFGRIGRLVFRALLSQKNIEIVAVNDLTHPDTLAHLLKYDSAHGEFKKKVVAKDNTLMIDKKKVLVFSEKDPANLPWAEHNIDIVVESTGRFVSEEGASLHLQAGAKRVIISAPAKQKTIKTVVYNVNHKIINAEDKIISAASCTTNCLAPMVHVLEKNFGILHGTMVTVHAYTADQRLQDAPHSDLRRARAAACNIVPTTTGAAKAIGLVVPEATGKLNGMALRVPVLTGSIVELCVALEKDATVEQINQAMKKAASASFRYCEDEIVSSDIVGSEHGSIFDSKLTNIIEVDGNKLYKVYAWYDNESSYVNQLVRVVNYCAKL" }, "paralog": false, "identity": 0.9970326409495549, "score": 608.0, "evalue": 6.64e-223, "description": "Internal stop codon at 516724. Nonsense mutation around 516724.", "psc": { "uniref90_id": "UniRef90_P75358", "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "ec_ids": [ "1.2.1.12" ], "uniref50_id": "UniRef50_P47543", "cog_id": "COG0057", "cog_category": "G", "go_ids": [ "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0016020", "GO:0016620", "GO:0031639", "GO:0034394", "GO:0050661", "GO:0051287" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75358", "EC:1.2.1.12", "UniRef:UniRef50_P47543", "COG:COG0057", "COG:G", "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0016020", "GO:0016620", "GO:0031639", "GO:0034394", "GO:0050661", "GO:0051287" ] }, "pscc": { "uniref50_id": "UniRef50_P47543", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47543" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" } }, "id": "CNOCLNGIAL_936", "locus": "GCA000143945_00921" }, { "type": "cds", "sequence": "contig_1", "start": 516722, "stop": 516991, "strand": "-", "frame": 2, "gene": null, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X0XC69" ], "nt": "ATGCTAGCAAAGAGTAAGACTATCCGTGTTGCTATCAATGGATTTGGACGTATTGGACGCCTTGTTTTTCGTGCACTTTTAAGCCAAAAAAATATTGAGATCGTTGCCGTTAACGACCTCACCCATCCCGATACTTTAGCACACTTACTAAAGTACGACTCCGCTCACGGTGAGTTTAAGAAAAAGGTAGTAGCTAAGGACAACACCTTAATGATTGACAAAAAGAAGGTGTTAGTGTTTAGTGAAAAAGATCCTGCTAACCTGCCATGA", "aa": "MLAKSKTIRVAINGFGRIGRLVFRALLSQKNIEIVAVNDLTHPDTLAHLLKYDSAHGEFKKKVVAKDNTLMIDKKKVLVFSEKDPANLP", "aa_hexdigest": "c1be61464e39cd0954b4529b83755861", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X0XC69", "query_cov": 0.9213483146067416, "subject_cov": 0.9425287356321839, "identity": 0.537, "score": 92.8, "evalue": 5.82e-23, "valid": false, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A2X0XC69" }, "pscc": { "uniref50_id": "UniRef50_A0A2X0XC69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X0XC69" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "CNOCLNGIAL_937", "locus": "GCA000143945_00922" }, { "type": "cds", "sequence": "contig_1", "start": 517151, "stop": 517723, "strand": "-", "frame": 2, "gene": "ecfT", "product": "putative MG302-like protein", "db_xrefs": [ "COG:COG0619", "COG:H", "GO:0005886", "KEGG:K16785", "SO:0001217", "UniRef:UniRef50_P47544", "UniRef:UniRef90_P75357" ], "nt": "TTGGCCAGTCAAAATGAAAATTTTTTAGGTCATTTTATTTATTCAGGCGGTGAACAGTTTGGTGGGGTGAGTTGGTGGAGTGTCAATACCCGCTCTTTACTACGTTCATTGGTAATAGCACTACGAATTAGCATGCTGTTTGCCACGTCCTTTCTCTTAACTGCGTCCACTTCGATTTATGAGTTAGCGTTTGGGGTCGAACGGTTGTGTACCCCTTTGCGCTACCTCAAAATTAAAACACAACCGTTAAGCATCTTATTTGCACTGGTGTTTAAACTGTTACCCATTGTTAAGGGTGAATTAAAACGGATTAAACAAGCGCAAGCAATCCGTGGTTTTAAGTACGGTAAGCTAGCCTTTTTAAATCCAGTCAAACTCAAAACGCTCTTTATTCCGGTGTTATTGTCAACGGTCAAAAAAACCGAGGCAGTCGCGTTTGCGCTACAAGCCAAGGGTTACCAGTTGGACAATCCTAATAAAACGCACTATCTCCAAAAGTACAACCTTTGGGGTGGGATAGTCTTTCTGGGGCTTTTTGTCTTACTTAGTTGCTTATTAATGGTGAACAACTGA", "aa": "MASQNENFLGHFIYSGGEQFGGVSWWSVNTRSLLRSLVIALRISMLFATSFLLTASTSIYELAFGVERLCTPLRYLKIKTQPLSILFALVFKLLPIVKGELKRIKQAQAIRGFKYGKLAFLNPVKLKTLFIPVLLSTVKKTEAVAFALQAKGYQLDNPNKTHYLQKYNLWGGIVFLGLFVLLSCLLMVNN", "aa_hexdigest": "a75fbd3f2fd7e37bc8f9df0b6be3664d", "start_type": "TTG", "rbs_motif": null, "genes": [ "ecfT" ], "truncated": "both", "pseudogene": { "start": 517022, "stop": 517975, "observations": { "mutation": [ 517138, 517153, 517792, 517921 ], "stop": [ 517138, 517153, 517792, 517921 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75357", "query_cov": 1.0, "subject_cov": 0.5993690851735016, "identity": 0.995, "score": 365.0, "evalue": 7.46e-126, "gene_start": 1, "gene_end": 190, "reference_start": 85, "reference_end": 274, "reference_sequence": "MTSKNFLSQLPPVLKLWWWIISLVVAFLPLGLHGLIIINAIFFALVIVVERKLKTFAIIFGWLLFFFWFNIVVNGFIFLPNSSALASQNENFLGHFIYSGGEQFGGVSWWSVNTRSLLRSLVIALRISMLFATSFLLTASTSIYELAFGVERLCTPLRYLKIKTQPLSILFALVFKLLPIVKGELKRIKQAQAIRGFKYGKLAFLNPVKLKTLFIPVLLSTVKKTEAVAFALQAKGYQLDNPNKTHYLQKYNLWGGIVFLGLFVLLSCLLMVNNWHLVYWTNPHYSFTFTHQNFCFFKQISSPQLLAFWQLELLAIG" }, "paralog": false, "identity": 0.9873817034700315, "score": 616.0, "evalue": 5.76e-227, "description": "Internal stop codon at 517138, 517153, 517792, 517921. Nonsense mutation around 517138, 517153, 517792, 517921.", "psc": { "uniref90_id": "UniRef90_P75357", "gene": "EcfT", "product": "Uncharacterized protein MG302 homolog", "uniref50_id": "UniRef50_P47544", "cog_id": "COG0619", "cog_category": "H", "kegg_orthology_id": "K16785", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75357", "UniRef:UniRef50_P47544", "COG:COG0619", "COG:H", "KEGG:K16785", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47544", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47544" ], "product": "Uncharacterized protein MG302" } }, "id": "CNOCLNGIAL_938", "locus": "GCA000143945_00923" }, { "type": "cds", "sequence": "contig_1", "start": 517965, "stop": 518894, "strand": "-", "frame": 1, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding MG303-like protein", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P75356", "UniRef:UniRef90_P75356" ], "nt": "GTGCACCCTTTTTATTATTTAACAGCTAAAAAACACACCCCGCTGTTTAACCAGCAGTTAGTTGATTTAGCGCAAAGTACGCTGTATTTTTACAACCTTAGTGTCTTTGTTGATAAAAGCAATGCTGGTCAAATTATTAAAAACGTTACCGGTAGTGTGGAACCTAACCAAATTACCGTTATTTTTGGTCCTAGTGGCAGTGGCAAAACGACCCTAATTAAACAGTTAGGTTTAGTTGAAAATCCGACCTGTGGTTTTTTGAACTGTGGTAACTTTTATTACTTTGCCAACCAAAAACATAACCGTGCCACAAAGCAGTTCCAAAACAGCATTGGCTATGTGTTACAAAAAGCCGAAGAACAGTTTTTCTGTGATTCCGTTTTAGAAGAGGTGTTAACGGGAGCGATTAACCTCGGTTTGTGTCAAAAGGGTGATGTCAACTTTGCCAAAAAGTACTTAGAGATGTGTGGTTTGGACCACATTCCCCTAATTAAAAACCCCTTGGAGTTGAGTGGCGGACAAAAGAAACGCTTGGCATTGGCGAGTGTATTAGCCATGCAAGTGCAATTTCTCATTTTGGACGAACCAACGGTCGGGTTAGACCAGGAGGGTAAAGCACTTAAAAGTGCTTTACTCAAACAATTAAAACAGGTCACGCGCATTATGATTGTCAGTCACGACGTTGATTTCATTTACGAAACCGCTGACAGTCTGATTCAATTAGAGGCTGGTCAAATTGTCGACCAAATGAGTGTTGCAGATTTCTTTAACAACATGCAGCTTTTACAACGGTACGAAATTACCCCACCACTTGTGGTGCAAACCATTCAGTTGTTACAAGCAAAGGGTGTGCAGTTAAACGATCCCTTAGCCATTAAAACCGTACACGATCTGATTGATCAACTCAAACCGCTCTTCCATGACCAGTAA", "aa": "MHPFYYLTAKKHTPLFNQQLVDLAQSTLYFYNLSVFVDKSNAGQIIKNVTGSVEPNQITVIFGPSGSGKTTLIKQLGLVENPTCGFLNCGNFYYFANQKHNRATKQFQNSIGYVLQKAEEQFFCDSVLEEVLTGAINLGLCQKGDVNFAKKYLEMCGLDHIPLIKNPLELSGGQKKRLALASVLAMQVQFLILDEPTVGLDQEGKALKSALLKQLKQVTRIMIVSHDVDFIYETADSLIQLEAGQIVDQMSVADFFNNMQLLQRYEITPPLVVQTIQLLQAKGVQLNDPLAIKTVHDLIDQLKPLFHDQ", "aa_hexdigest": "6fda083b9336e7474d6d4b2bc34b984a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75356", "query_cov": 1.0, "subject_cov": 0.8089005235602095, "identity": 0.997, "score": 611.0, "evalue": 9.55e-220, "valid": true, "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG303 homolog", "uniref50_id": "UniRef50_P75356", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P75356", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75356" ], "product": "Putative ABC transporter ATP-binding protein MG303 homolog" }, "genes": [ "ecfA2" ], "id": "CNOCLNGIAL_939", "locus": "GCA000143945_00924" }, { "type": "cds", "sequence": "contig_1", "start": 519110, "stop": 519820, "strand": "-", "frame": 2, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding MG304-like protein", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47546", "UniRef:UniRef90_P75355" ], "nt": "TTGCTCAACGTTACCAACCTCAGTTTTACCTATCCGCGCCACAGTCAACCGGCCTTAGAAAAGCTCAATTTTAGCCTCAAACCCAACACCCATGTAGCCATTATTGGCCACAATGGTTCGGGTAAATCGACACTGGTCAAACTGTTAGGCGGATTTTTAAAAGCACCGAAACAAACAATCTTTTTTAGGGGTCAACCCTTGGAAGAAGTTGGTTTTCGCCAAATTGGTATCTTACTGCAAGATCCCGACATGCAACTGTTGGGTGATACGCTGCACCAAGAGCTCATTTTTAGCTTGGAAAACCACGGGGTGGATCCTAAACAAATGGACCGAGTGATTGCTGATGTTTTAGCGGTTGTAGAACTACAAGGTAAACAGTTCACACCGCTCAGTAAGCTGTCGTTTGGCGAAAAACAACGGGCCGTGTTTGCTTGTTTACTGGCGGTGAAACCCGAACTTTATTTACTAGATGAAGCCTTTAGTATGTTGGATGGTAAGCTAGCTAGCAAACTAAAGCGGTTCATTTTTAAGGTAATTAAAGAACAGCAAAAAACAGTTATTAATGTTACCCATGACTTTAATGACCTCTTCTTAGCGGATGAAATTATCTTTTTAAGTAAGGGACAGCTGTTAAAACAGTTTTCACCCGCTGCCATATACAAACAGCTCCACTTGTTCCACCAGCACCACTTTACCCTCCCGTTTCCATGA", "aa": "MLNVTNLSFTYPRHSQPALEKLNFSLKPNTHVAIIGHNGSGKSTLVKLLGGFLKAPKQTIFFRGQPLEEVGFRQIGILLQDPDMQLLGDTLHQELIFSLENHGVDPKQMDRVIADVLAVVELQGKQFTPLSKLSFGEKQRAVFACLLAVKPELYLLDEAFSMLDGKLASKLKRFIFKVIKEQQKTVINVTHDFNDLFLADEIIFLSKGQLLKQFSPAAIYKQLHLFHQHHFTLPFP", "aa_hexdigest": "d6dc03ce116bce752013486a5f58541f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75355", "query_cov": 1.0, "subject_cov": 0.8676470588235294, "identity": 0.996, "score": 466.0, "evalue": 2.48e-165, "valid": true, "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG304 homolog", "uniref50_id": "UniRef50_P47546", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P47546", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47546" ], "product": "Putative ABC transporter ATP-binding protein MG304" }, "genes": [ "ecfA2" ], "id": "CNOCLNGIAL_940", "locus": "GCA000143945_00925" }, { "type": "cds", "sequence": "contig_1", "start": 520038, "stop": 521765, "strand": "-", "frame": 1, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_P9WMJ9", "UniRef:UniRef90_P47547" ], "nt": "ATGAGTACAGATAACGGCTTAATTATCGGCATTGACCTTGGTACCACTAACTCCTGTGTGTCGGTCATGGAGAATGGACGCCCAGTAGTGTTGGAAAACCCTGAAGGTAAACGCACCACCCCTTCGATTGTTTCTTACAAGAACAACGAAATTATTGTGGGTGATGCTGCGAAACGGCAAATGGTAACTAACCCTAATACTATTGTTTCCATTAAGCGTTTAATGGGTACCTCCAATAAGGTAACCGTTAAGAATCCTGATGGTTCTACCAAAGAGTTAACTCCTGAAGAGGTATCAGCGCAAATCTTGAGCTACCTCAAGGACTATGCGGAAAAGAAGATTGGTAAAACGATTTCCCGTGCTGTTATTACCGTACCTGCTTACTTTAACGATGCAGAACGGAACGCTACTAAAACCGCTGGTAAGATTGCTGGTTTAAACGTTGAGCGGATTATTAACGAACCTACCGCCGCTGCATTGGCTTATGGGATCGACAAGTCTAACCGAGAAATGAAAGTCTTGGTGTACGACCTTGGTGGTGGTACCTTTGACGTTTCCTTACTTGACATTGCTGAAGGTACCTTCGAAGTATTAGCCACTGCTGGGGACAACCGTTTGGGTGGTGATGACTGGGACAACAAGATTATTGAGTTCATCTTAGCGCACATTGCCCAAGAACACAATGGGCTTAACTTGTCCAATGACAAGATGGCTATGCAACGCTTAAAGGAAGCGGCTGAACGTGCTAAGATTGAACTTTCCGCCCAACTAGAAGCAATTATCTCTTTACCGTTCTTAACGGTTACCGAAAAGGGTCCGGTAAACGTTGAACTTAAGCTAACCCGTGCTAAGTTTGAAGAAATTACCAAACAATTACTAGAACGTACTCGCAACCCAATTTCGGATGTTTTACGTGAAGCCAAGATTAAACCAGAAGAAATTAATGAAATCTTGTTGGTGGGTGGATCGACCCGGATGCCAGCAGTGCAAAAACTAGTGGAATCAATGGTACCAGGACACAGTCCAAACCGCTCAATTAACCCGGATGAGGTGGTAGCCATTGGTGCTGCCATCCAAGGGGGTGTGTTACGCGGTGATGTAAAGGACGTGTTACTGTTGGACGTTACTCCTTTAACGCTCTCGATTGAAACCCTTGGTGGTGTAGCAACTCCGTTAATTAAGCGTAACACCACCATTCCTGTAAGTAAGAGTCAAATCTTCTCTACAGCGCAAGACAACCAAGAATCAGTGGATGTGGTGGTTTGTCAAGGGGAACGCCCAATGGCACGTGACAACAAGTCTTTGGGTCGCTTTAACTTAGGGGGCATCCAACCAGCACCCAAGGGTAAACCCCAAATTGAAATTACCTTTAGCTTGGACGCCAACGGGATCTTAAACGTGAAGGCTAAAGATTTAACCACTCAAAAGGAAAACAGTATTACTATTAGTGACAACGGCAACTTGTCCGAAGAGGAAATCCAAAAGATGATTCGTGATGCGGAAGCCAACAAGGAGCGTGACAATGTGATTCGTGAACGCATTGAGCTCCGTAACGAAGGTGAAAGCATCGTGAGCACGATTAAGGAGATTCTCCAAAGTCCCGAAGCGAAGGACTTCCCTAAAGAAGAGAAGGAAAAACTCGACAAGATTACCGGTGGTATTGATGCAGCAATTAAGGCCAATGACTACACCAAGTTAAAAGCCGAAATCGAAAACTTCAAGAAGTGA", "aa": "MSTDNGLIIGIDLGTTNSCVSVMENGRPVVLENPEGKRTTPSIVSYKNNEIIVGDAAKRQMVTNPNTIVSIKRLMGTSNKVTVKNPDGSTKELTPEEVSAQILSYLKDYAEKKIGKTISRAVITVPAYFNDAERNATKTAGKIAGLNVERIINEPTAAALAYGIDKSNREMKVLVYDLGGGTFDVSLLDIAEGTFEVLATAGDNRLGGDDWDNKIIEFILAHIAQEHNGLNLSNDKMAMQRLKEAAERAKIELSAQLEAIISLPFLTVTEKGPVNVELKLTRAKFEEITKQLLERTRNPISDVLREAKIKPEEINEILLVGGSTRMPAVQKLVESMVPGHSPNRSINPDEVVAIGAAIQGGVLRGDVKDVLLLDVTPLTLSIETLGGVATPLIKRNTTIPVSKSQIFSTAQDNQESVDVVVCQGERPMARDNKSLGRFNLGGIQPAPKGKPQIEITFSLDANGILNVKAKDLTTQKENSITISDNGNLSEEEIQKMIRDAEANKERDNVIRERIELRNEGESIVSTIKEILQSPEAKDFPKEEKEKLDKITGGIDAAIKANDYTKLKAEIENFKK", "aa_hexdigest": "c4c9d98b7612066c9a72eb32f77d0427", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47547", "query_cov": 1.0, "subject_cov": 0.9663865546218487, "identity": 0.934, "score": 1022.0, "evalue": 0.0, "valid": true, "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_P9WMJ9", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_P9WMJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P9WMJ9" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "CNOCLNGIAL_941", "locus": "GCA000143945_00926" }, { "type": "cds", "sequence": "contig_1", "start": 521980, "stop": 522297, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGGTTTAGAAATTAAACGTTTAGGCTTGACGATCTTCAAATTACTAGCCAATATCTTAATAGCAGTGGTGGGTTACTTTAGTGTGTTTGCTGTTTTTGCCATTGTCAAAAAGGAAAACGCAACTAACAACGTTATTGACCAGTACTCTACGGCTGACATTTTCAACATCACTAACATTGCCATTACCGCTTCTAACTTTATTCCCGTACCTAGTGGGGAAGGTGCTACCCAGTTTGTAATGACAAGCTTTTTAAATGCCTTTAAATCGGCTGTTGGTATTGAATCGCAAGTAAAGCAAGGGGTCTTTTTGTGA", "aa": "MYGLEIKRLGLTIFKLLANILIAVVGYFSVFAVFAIVKKENATNNVIDQYSTADIFNITNIAITASNFIPVPSGEGATQFVMTSFLNAFKSAVGIESQVKQGVFL", "aa_hexdigest": "706b7193aaa74d2f01bbae0e318addc0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11385.160599999992, "isoelectric_point": 8.00776309967041 }, "id": "CNOCLNGIAL_942", "locus": "GCA000143945_00927" }, { "type": "cds", "sequence": "contig_1", "start": 522367, "stop": 522468, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTGATGTGGTGGTAGCCATCTTATTTATCTTTATTGCCTACAGTAAAAGGATGCATGTCTTAATTTACTCCGGGGTAAACCAGTTTCGTAAGTGA", "aa": "MIFDVVVAILFIFIAYSKRMHVLIYSGVNQFRK", "aa_hexdigest": "38a2d1f03e38d08d6a172cce833d66d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3919.7433, "isoelectric_point": 9.992035484313966 }, "id": "CNOCLNGIAL_943", "locus": "GCA000143945_00928" }, { "type": "cds", "sequence": "contig_1", "start": 522556, "stop": 522711, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTCTTTCAAATTGTCACCCCACTTTCCTTGGGTCAAGATCCCTTTCGCTTGTACTGGTTCATTAAAAAGGGCATGAAGAAGCAGACAGCGGTCTTATTAGTTACTTCCACGGGCGCCTTTTGGAACCTTGCCCAAGCGTTAATTACTTGA", "aa": "MQFFQIVTPLSLGQDPFRLYWFIKKGMKKQTAVLLVTSTGAFWNLAQALIT", "aa_hexdigest": "a1c0539050506c6ffe39ed05945a04fb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5848.919200000001, "isoelectric_point": 10.171064186096192 }, "id": "CNOCLNGIAL_944", "locus": "GCA000143945_00929" }, { "type": "cds", "sequence": "contig_1", "start": 522841, "stop": 523008, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAACCCCTAAAAGCGCTAGTTTTTTTACTAGAAAAAATATTCTTGCCTTTAGCTTTTTTATTGCCTTTTTGGTGGTTGTTAGTGTATTGGTGACCGTCTTCTTTTTAGACATTAAAACTGGTGATGTTAAAACGATTATTAACACCATTAACAGAACTAACTGA", "aa": "MSTPKSASFFTRKNILAFSFFIAFLVVVSVLVTVFFLDIKTGDVKTIINTINRTN", "aa_hexdigest": "e0141c928abccabe149aedb1b9e4099d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6174.254499999999, "isoelectric_point": 10.446408653259276 }, "id": "CNOCLNGIAL_945", "locus": "GCA000143945_00930" }, { "type": "cds", "sequence": "contig_1", "start": 523018, "stop": 524472, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_437", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75341" ], "nt": "TTGGAAAACATCCAAACTAAAGTAAATGATTTTGTTAAAAACAAACTCAAACCTGCTTTAGCACCCCGACCAGCTTACAGTAACGTCATTTTGTTAAACGTTAACAATGACAAAGTACTTTCTAGTGGTGCTAACTGGAGCTTAGCTAGCTTATTACAGAGCGACAAAGTCAACCCGCTTTCGTTTATGTTGCTCAAACAAGCGTTCGATAATAACGATTTATTTAAGAAAGCACAAAAGTTGTTTAAAGACATTCAGGAAAAGTCCAGTAATAATGGTGGAATGCAAAGTAGTTCCACAACCAACAGTGATGCCGATGCATTAAGTAAAGTTATCGGTAACTATTACTACACTACTTGGGCTAAACTCACGGACAAATCGATTTACGGTAACCCGAAGGACAACAAGTTTGATGAGCTTTTCAAACTGGCTTTTGAAGCTAGCATCGACGAAAAGTCCTTTAACGTTGATTACAAAGCGGTCATTGACCATTACCGTTTTATCTATACCTTACAGTGGCTAGTGGACCAAAAATTAAAGAACTTTAAGTCACTGTTAAAAACAAACCTTAAGTTTGGTGAAGTTGCTTTTATAGCTTACAAGAACACTGAAACCACTAACTTCTCTAATCCCCAAGGCGTATTTGGTTCCTACTTCAACTATGAGAACTCCGCTAACGAAGTTAAAGAATCCACTCAAACACTAGATCCCAATAACTTCTTTTACAAAACAACAACCAAGCCAACGGTACAAGCCACTCAACAAGTTGCTAGCTTAGCATTAGTACAAAAACAACAAATGCAACAAAATTCAACTGATCACTATGGTTTTACTGGTTTGAGTACCAGCACTAGTTCGATGTTCGATGCTAGTTCCCGTGATGCCATTTTGCAGCAAATTACGAAAACCTCCTTGCAACAGTACGGTTCCAAGGATCAACTCAAAAAGATCATTCAAGGGACTAATAACCAATTGTTACTAGACCGGATTGCAGTCCAGTTAAGTGGATTAAATCCTTCCACAACTAATGGTGGCAGTGGCAAAACAATTGCGACCTACTTCCAAGTGGATGCCGTTGGCAACCCAACTTTGGACTTCCAAGCTAAGCGTCAACTGTTATTAGACCTCTTGGACCAGTACCAAAACTACTTTGGTAATGGTGCCCAAAAGAGCCAAAGGGATTCTACTCCAAGTGGAACTGGCAACTACCTCACTTACCAAAATGGTAGTGACAAGTACACCTACACCCAGTTCACTTACCAAGACATTGACAGCTTGAGTCTAACAACTACAAGCGGTACCAACAATAAAATTGCCAGTGATGTTGTGGCAGCGCTGCTTTTATTCCAAGCAGCAGACAAGGGCACACAACAGTTGGCACTTAGTGCCATTAATAAGCCGCAGTTAAATATTGGTGATAAACGGATAGAAAGTGGGTTAAAATTGCTCAAATAG", "aa": "MENIQTKVNDFVKNKLKPALAPRPAYSNVILLNVNNDKVLSSGANWSLASLLQSDKVNPLSFMLLKQAFDNNDLFKKAQKLFKDIQEKSSNNGGMQSSSTTNSDADALSKVIGNYYYTTWAKLTDKSIYGNPKDNKFDELFKLAFEASIDEKSFNVDYKAVIDHYRFIYTLQWLVDQKLKNFKSLLKTNLKFGEVAFIAYKNTETTNFSNPQGVFGSYFNYENSANEVKESTQTLDPNNFFYKTTTKPTVQATQQVASLALVQKQQMQQNSTDHYGFTGLSTSTSSMFDASSRDAILQQITKTSLQQYGSKDQLKKIIQGTNNQLLLDRIAVQLSGLNPSTTNGGSGKTIATYFQVDAVGNPTLDFQAKRQLLLDLLDQYQNYFGNGAQKSQRDSTPSGTGNYLTYQNGSDKYTYTQFTYQDIDSLSLTTTSGTNNKIASDVVAALLLFQAADKGTQQLALSAINKPQLNIGDKRIESGLKLLK", "aa_hexdigest": "919334446a07c5d82f1198222e4619ab", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75341", "query_cov": 0.9772727272727273, "subject_cov": 0.8269230769230769, "identity": 0.7190000000000001, "score": 634.0, "evalue": 9.21e-223, "valid": false, "product": "Uncharacterized protein MPN_437", "uniref50_id": "UniRef50_P75341" }, "pscc": { "uniref50_id": "UniRef50_P75341", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75341" ], "product": "Uncharacterized protein MPN_437" }, "genes": [], "id": "CNOCLNGIAL_946", "locus": "GCA000143945_00931" }, { "type": "cds", "sequence": "contig_1", "start": 524566, "stop": 525654, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGCAAATTAAGGAGCTCAAAGAAGACCTTAACAACACGATTGTTGTCGATAAAAGTAAGGACGTAGAAAAAGCTTCCAAAACTAACGCACTGTTTTACAGTGATCAAGAAGGTAAGCAACAACAAAGTGACTCAGATCCAATTGCTGGCGCTTTAGATGACATTTTTGCTCAAAACACAAGCGAAGGCACTAACTTAAGTAAGTTAGCGGAGCAGGTAAAGAAAGCAGCTGCAACAAAAATGGAAGCGAAAACAGCTGTTTTAAGAACTAACAATTCTAAGGGCCAACAAAACAATTACGTTGTTTTAGATGCAGCTATTCCTACATTTAATTCAACAACATCAAAATCGAAAAATAATAGCGCTTCTAATGAAGTTTTAGTTGCCTTAAAATCTGGATCTATAAATTTAAGGCAGGTTCAGCAAACTGATCAAAACAGTTACAGTCCCATTAAATTCCGGATTGTGCGTAATAGCACCGGAGTAACTGTTTTCGGCCTTGATGGTGGGAGCTATTATTTAAAACAAGATTCAACAAATAAAAAATCAGTTTCTAAGCAAAGTTTAACCTTATTAACTAAATCTAGTTCAGGTAACAGTAATAAAGTATTAAGAGACCTTGACAAGCAAAAACAATTCTTAAAGTTTCGTGCCTTTCAAGCTAAAACTAACACTTTCTACAGTACTAACTTTGCCTTTAGTTTTCCCTTAAACGAAACGCTAAAAAGTTGGTTTGATAAACACCGGGAACTAATTTTGGCCAACGCCTTAGTTAACGCTAGTCTTGACCAAAAGGATAAAGCTAGCAAAGCTTTAACTGAAGCTTTTAATCCTTATAAAGAGTTAATTAAAGAATTTGCGCCGGTGGCTTTAGCAACCACAATGATCAGCTTTTATTTTGATCAAATGAAAGCGCTCAATAACAAGTTGCTAGAGCGCGCCCGTAATCTTAACCAAAACGTCAACCAAGCCAATCCAACCCCTTGGTTGAACGGTTTGTCAGCCAAGTTACCTTACGTTAATACTAACGGTAATTACGAAAAGTTAAACAACTACTTTACCTTCTTAATCACAAAAACATTGTGA", "aa": "MPQIKELKEDLNNTIVVDKSKDVEKASKTNALFYSDQEGKQQQSDSDPIAGALDDIFAQNTSEGTNLSKLAEQVKKAAATKMEAKTAVLRTNNSKGQQNNYVVLDAAIPTFNSTTSKSKNNSASNEVLVALKSGSINLRQVQQTDQNSYSPIKFRIVRNSTGVTVFGLDGGSYYLKQDSTNKKSVSKQSLTLLTKSSSGNSNKVLRDLDKQKQFLKFRAFQAKTNTFYSTNFAFSFPLNETLKSWFDKHRELILANALVNASLDQKDKASKALTEAFNPYKELIKEFAPVALATTMISFYFDQMKALNNKLLERARNLNQNVNQANPTPWLNGLSAKLPYVNTNGNYEKLNNYFTFLITKTL", "aa_hexdigest": "4fba2028c855791ba036ed9cbad188d9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 40403.07009999999, "isoelectric_point": 9.588463401794431 }, "id": "CNOCLNGIAL_947", "locus": "GCA000143945_00932" }, { "type": "cds", "sequence": "contig_1", "start": 525916, "stop": 526101, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGTACACTGATCCCACTTTAATTAGTCAAGTTAACTATAAGTATTCTGCTCCCAGTCAAGGGTTAGGTCAGATCTATAACAGAGAGAAGTTAAAGGATAAACTAACACCTTCTTATGCCTTTCCTTTCTTTGCCGAAGAAAAAGACATTGCACCCAACCAAAACGTTGGTAATAAGCGCTGA", "aa": "MEYTDPTLISQVNYKYSAPSQGLGQIYNREKLKDKLTPSYAFPFFAEEKDIAPNQNVGNKR", "aa_hexdigest": "cf66cb80295171112421c4c0f42a0c3f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7015.779500000002, "isoelectric_point": 7.961990547180176 }, "id": "CNOCLNGIAL_948", "locus": "GCA000143945_00933" }, { "type": "cds", "sequence": "contig_1", "start": 526189, "stop": 526554, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_439", "db_xrefs": [ "GO:0098552", "SO:0001217", "UniRef:UniRef50_P75339", "UniRef:UniRef90_P75339" ], "nt": "GTGGCCAACAAACAATTTATCGCTGCACCACTTTTAAAAGCACTAGAAGCTTGGTACGAAAACAACGAAGACAAAAAGATTACCCAGTTTTTAAAAGACACTAAGTCCAATGTTGACAGTCAGTACACAACGGCAGTCGATAAAGTGGTATCAGCATCACGCAATAAATCACTTTTTGTGCAACAAGATTTGTTGGATAACGCCGGTGGTAGTGAAGCAACCTGGAAAGCGCAAAAGCTGCTTGAACAGCTCATTAGTGACTTTGCTAGTCGGGTTTTCCAAAAGAACTACCTCAATTACAAAAAAGATGGACAAGTTTCTACTGGTCCATTTACTTATGATGAACTACACAAGGAAGAAAGCTGA", "aa": "MANKQFIAAPLLKALEAWYENNEDKKITQFLKDTKSNVDSQYTTAVDKVVSASRNKSLFVQQDLLDNAGGSEATWKAQKLLEQLISDFASRVFQKNYLNYKKDGQVSTGPFTYDELHKEES", "aa_hexdigest": "f6b67903329ec1df8755fa34cab58ee5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 525926, "stop": 526752, "observations": { "deletion": [ 525966 ], "mutation": [ 526191, 526722 ], "stop": [ 526191, 526722 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75339", "query_cov": 0.9586776859504132, "subject_cov": 0.4218181818181818, "identity": 0.81, "score": 192.0, "evalue": 1.8e-59, "gene_start": 3, "gene_end": 118, "reference_start": 69, "reference_end": 184, "reference_sequence": "MKSFLRKPKFWLLLLGGLSTSSIILSACATPSNSALQAVFKPTSNQFFNGEHGTIQSALNTALRDPETNKKFVAAPLLKALEAWYENNQDKNITQFLKDTKTNVDNQYKTVVDKVVSAPRNKSLFVQQDLLDSSGGSEATWKARKLFEQLISDFASRVFQKNYLSYKENGKVSAGPFLYDTISKNSNWQNIVFDAGNFPETNDDFFAQIQSEVFDQWAEYTDPTIISSVTLKYSAPNQGLGQIYNKDKLKDKLTPSYAFPFFCWGKRYSGKSKRW" }, "paralog": false, "identity": 0.7381818181818182, "score": 414.0, "evalue": 7.25e-149, "description": "Internal stop codon at 526191, 526722. Frameshift due to deletion around 525966. Nonsense mutation around 526191, 526722.", "psc": { "uniref90_id": "UniRef90_P75339", "product": "Uncharacterized lipoprotein MPN_439", "uniref50_id": "UniRef50_P75339", "go_ids": [ "GO:0098552" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75339", "UniRef:UniRef50_P75339", "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75339", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75339" ], "product": "Uncharacterized lipoprotein MPN_439" } }, "id": "CNOCLNGIAL_949", "locus": "GCA000143945_00934" }, { "type": "cds", "sequence": "contig_1", "start": 527000, "stop": 527683, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATGCAACAAAAAATGCAAAGTACCATGCAAGATAGTAATCATTATGGTTTTACCGGGCTAAACACCAGTACTAGTTCAATGTTAGGTGCCGCTACGCAGCAAGCCATTTTGGATCAAATAACCAAGAATTCCTTGCAACAATATGGTTCGCAGCAAGAACTGAAAACTTTGATTGAAAAGACTAATAACCAACTTTTATTAGACCGAATCGCTAGTCAATTAAGTGGATTAAATCCTTCAACCACTGGAAATAGTAATAATGGAAAGGGTAAAAATATTGCTACTTATTTCCAATTAGATGCCATTGGCAACCCAACCCTTAGCTTTCAACAAAAACGAAAACTTTTATTAGATGTTTTAGATCAGTACAAAGATTTCTTTGGTACAAATACTCAGGCTGCACAAAGAGATTCTGGTAAAGGTGGACATGGAAGCTATTCAACTTACCAAGATGGCAGTGACAAGATCACTTACCTTCAGTTCTCCTATAAAGATATAGATAACTTAAGTTTGAGTGATAAAGGAAATAGTAAGCTTGCTAGCGATGTTGTGGCTGCCCTTTTACTTTTCCAAGCAGCGGATAAAGGTACCCAACAATTAGCCTTGAGTGCTATTAATAAACCACAACTACCAATTGGTGATAAGCGGATTAAAACAGGAATAGATTTACTTAAATAG", "aa": "MVMQQKMQSTMQDSNHYGFTGLNTSTSSMLGAATQQAILDQITKNSLQQYGSQQELKTLIEKTNNQLLLDRIASQLSGLNPSTTGNSNNGKGKNIATYFQLDAIGNPTLSFQQKRKLLLDVLDQYKDFFGTNTQAAQRDSGKGGHGSYSTYQDGSDKITYLQFSYKDIDNLSLSDKGNSKLASDVVAALLLFQAADKGTQQLALSAINKPQLPIGDKRIKTGIDLLK", "aa_hexdigest": "a6a0378525066007380e86d7be863627", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24783.535799999987, "isoelectric_point": 9.097665596008301 }, "id": "CNOCLNGIAL_950", "locus": "GCA000143945_00935" }, { "type": "cds", "sequence": "contig_1", "start": 527945, "stop": 528097, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGATAAATCCATTTATGGAAATCCTAAGGACAACAAATTTGATGACTTATTTAAATTAGCTTTTGAAGATAGTATTAACGAAAAGTCCTTTAATGTTGATTACAAAGCGGTCATTGAACACTACCGCTTTATCTATACCTTGGAATGA", "aa": "MTDKSIYGNPKDNKFDDLFKLAFEDSINEKSFNVDYKAVIEHYRFIYTLE", "aa_hexdigest": "6489b238ee66db2e3891a3bff0400f39", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6013.649899999999, "isoelectric_point": 4.9303506851196275 }, "id": "CNOCLNGIAL_951", "locus": "GCA000143945_00936" }, { "type": "cds", "sequence": "contig_1", "start": 528104, "stop": 528286, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTCAAACAAACGTTTGACCAAAACGACTTGTTTAAGAAAGCTAAGAAACTTTTTGAAGATATTCAAAGTAAAACAAATGGTGGAAGTTCAGGTGGGATGCAAGGTAGCAATACCTCGAGTAGTGAAGGAGCTGATGCCTTGAGTAAAGTAATTGGCAACTATTACTACAACAGTTGA", "aa": "MLLKQTFDQNDLFKKAKKLFEDIQSKTNGGSSGGMQGSNTSSSEGADALSKVIGNYYYNS", "aa_hexdigest": "162176d8455fd8b24da4fbb08368e614", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6501.096200000004, "isoelectric_point": 7.9830717086792005 }, "id": "CNOCLNGIAL_952", "locus": "GCA000143945_00937" }, { "type": "cds", "sequence": "contig_1", "start": 528353, "stop": 528886, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAGGCAAATTTTGATCAAAAGGATAAAGCAAACAAAGCTTTAACTGAAGCCTTAAAGCCTTACAAGGATTTACTGGCAGAATATGCACCAATTGGTTTGGCCACAACCATGATTAACTTCTTGTTTAACCAAATGAACGCGTTCAATAAGTTTTTGGAGCGCGCTAAAGCATTAAACCAAAATGTGAATCAGGCCAATCCTACACCTTGGTTAAATGGTTTATCAGCTAAATTGCCTTATGTTAGAACAAATGGTAATTACGAAAAACTCAACAATTACTTCACTTTCTTAATTGTTAAATACATGTGGAAGAAGGTCGGTAATGAAGACGCTTCATTATCTAAAGATAGTAGCACTAACAAGCTCAAAACTAAGACCGAAGACGTTAACAAAATTAGAGATAAAATCTTAGAAGACATTCAAAAGAAGGTTCAAGAATTTGTTAAAAACAAGCTTAAACCAACCTTAGCACCACGACAAACTTACAGTAATGTAATTTTGTTAAACGTTAACAACGATAAGGTTTGA", "aa": "MVKANFDQKDKANKALTEALKPYKDLLAEYAPIGLATTMINFLFNQMNAFNKFLERAKALNQNVNQANPTPWLNGLSAKLPYVRTNGNYEKLNNYFTFLIVKYMWKKVGNEDASLSKDSSTNKLKTKTEDVNKIRDKILEDIQKKVQEFVKNKLKPTLAPRQTYSNVILLNVNNDKV", "aa_hexdigest": "cdf9a0eef25e221c927749cd8536c321", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20337.24010000001, "isoelectric_point": 9.718044853210447 }, "id": "CNOCLNGIAL_953", "locus": "GCA000143945_00938" }, { "type": "cds", "sequence": "contig_1", "start": 528923, "stop": 529378, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACAATCTGATGAAAAATATGTGTTATTAGATGCTGCGATCCCGGCGTTTGAAACTTCTTCACAATCACCACAAAGCAGTGGAACTATTTCTGGTAGTGGCGCTTTAGTTAATTTGACTTCTACTAGCGTCAATTTAACTCAACAAAAAAGTAGTACAACGCCTTTAAACCAGCGCATTGTTCGAAATAGTACTGGTGTAAAATTGCTGGCTCTTGAAGGCTTGCTTTACTACGTTGTTGGATCTTCCAATAAACAACAAAGTAGCTCTTCTACGATAGCCACTTCTCTCGCTCAAAACCAAAGTAATAATAATCAAGAACAAGAGATTAATAGCAGTCTTCAAAAAAATTTATTGAAGTTTCGTGCCTTTCAAGCTAAAAATAGAGTTTTCAAACAAGGCGGCACCAAATATGCCTTTTCGTTTCCGTTAAATGAAACCTTGAAAGGTTGA", "aa": "MAQSDEKYVLLDAAIPAFETSSQSPQSSGTISGSGALVNLTSTSVNLTQQKSSTTPLNQRIVRNSTGVKLLALEGLLYYVVGSSNKQQSSSSTIATSLAQNQSNNNQEQEINSSLQKNLLKFRAFQAKNRVFKQGGTKYAFSFPLNETLKG", "aa_hexdigest": "cd443c0a8be3ca122be5bb461a415f76", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16270.939899999985, "isoelectric_point": 9.742027091979981 }, "id": "CNOCLNGIAL_954", "locus": "GCA000143945_00939" }, { "type": "cds", "sequence": "contig_1", "start": 529415, "stop": 529660, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGACACCATTGTAGCTAAATCTGATAACTTAGAGAAAACTTCTAAAACTAACAGTACTTTTTACAAAGATAATGGTGATAGTAGCGAAAAACAAGGAAGTGCTGAAAGCAAAAATTCACCTAAAACTCAATTAAAGGAAATTTTTAATGGTCAAAATGGCGATAGTGGTAATTTAGGTAAGCTAGCTGAACAGTTACAAAAAGCTGACAGTACTAAAATGGAAGCTAGAAGTCAGACATGA", "aa": "MNDTIVAKSDNLEKTSKTNSTFYKDNGDSSEKQGSAESKNSPKTQLKEIFNGQNGDSGNLGKLAEQLQKADSTKMEARSQT", "aa_hexdigest": "4fb514a8a4301a9f7423220b1822eac3", "start_type": "TTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8802.437799999998, "isoelectric_point": 6.217185401916503 }, "id": "CNOCLNGIAL_955", "locus": "GCA000143945_00940" }, { "type": "cds", "sequence": "contig_1", "start": 530373, "stop": 530756, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_442", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "nt": "TTGTTGGGTGGCCTTTCCACTAGTAGTATTATTCTCAGTGCCTGTGCAACACCTTCAAACTCAGCACTGCAAGCAGTTTTTAAACCAACTTCCAACCAATTTTTTAACGGTGAGCATGGTACCATTCAAAGCGCTTTAAACACCGCTTTAAGAGATCCGGAAACTAACAAAAAATTTGTAGCTGCTCCACTTTTAAAAGCATTAGAAGCTTGGTACGAGAATAATCAAGATAAAAACATTACTCAATTCTTAAAAGACACCAAGACTAATGTTGATAACCAATACAAAACCGTTGTAGATAAAGTAGTTTCAGCACCACGCAATAAATCCTTATTTGTACAACAAGATTTATTGGACAGTAGCGGTGGTAGTGAGGCCACTTGA", "aa": "MLGGLSTSSIILSACATPSNSALQAVFKPTSNQFFNGEHGTIQSALNTALRDPETNKKFVAAPLLKALEAWYENNQDKNITQFLKDTKTNVDNQYKTVVDKVVSAPRNKSLFVQQDLLDSSGGSEAT", "aa_hexdigest": "3c229614f401cf9a42d083d70b5c7567", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75336", "query_cov": 0.9606299212598425, "subject_cov": 0.8079470198675497, "identity": 0.762, "score": 182.0, "evalue": 4.37e-57, "valid": false, "product": "Uncharacterized lipoprotein MPN_442", "uniref50_id": "UniRef50_P75336", "go_ids": [ "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "product": "Uncharacterized lipoprotein MPN_442" }, "genes": [], "id": "CNOCLNGIAL_956", "locus": "GCA000143945_00941" }, { "type": "cds", "sequence": "contig_1", "start": 530956, "stop": 531987, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_440", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75338", "UniRef:UniRef90_P75338" ], "nt": "TTGGCTTTTGACAGTAGTATTAACGAAAAGTCCTTTAATGTAGATTATAAGGCAGTGATTGAACACTACCGCTTTATCTATACCTTAGAGTGGTTGGTAGACAAAAACCTAAAGAACTTCAAGGATTTATTAAAGGCAAACCTCAAGTTTGGTGAAATTGCTTTTATTGCTTACAAGAATACTGAAACGCAGAACTTCTCTAACCCGCAAGGTATATTCGGTTCTTACTTTAATTACGAAAACGAAACGAATGCGGCTAAGAGTGCTACGCAAATTATAGACCCCAACAGTTTCTTCTATAAAACCACCACTAAACCAGAAGCGAAAACCACTCAAAGTGCTAATACAGCTGTGATGGTTCAAAATACGCAGATGAACAATCAGCAAACTAACAGTTATGGCTTTACTGGTTTGAGTACCAGTAGTGGTTCGATGTTAGGTGCTGCTACCCAGCAAGCCATTTTGGATCAAATAACCAAAACTTCCTTGCAACAGTATGGTTCTCAAGCTGACCTGAAAAAGATCATTGGCGAAACTAAAAATCAATTATTATTAGACCGAATTGCCAACCAACTAATAGCCTTAAAACCGAATACAAGTGGCAACAGTGGTACCCAAAAAACAATTGCTGCATACTTCCAAACAGATGCGGTTGGCAATCCTACTTTGGACTTTAAAGCGAAGCAAAAACTCTTATTGGATGTTTTAGATCAATACAAAGATTTCTTTGGTAATAATGCACAAGCAGTTCAAAGAGATTCTGGTAAGAGTGGAACTGGCAACTATTTAACCTATACCGACGGTAGTGATAAGATCACTTATTTGCAGTTTTCCTATAAAGATATTGACGGTTTAAGTTTGAGTAGTTCAAACGGAACTAGCAGCAAATTTGCCAGTGATGTTGTAGCAGCGCTTTTATTATTCCAGGCAGCCGATAAAGGTACTCAACAACTGGCTTTAAGTTCCATCAATAAACCACAATTACCAATTGGCGATAAACGCATAAAAACAGGGATCGATTTACTGAAATAG", "aa": "MAFDSSINEKSFNVDYKAVIEHYRFIYTLEWLVDKNLKNFKDLLKANLKFGEIAFIAYKNTETQNFSNPQGIFGSYFNYENETNAAKSATQIIDPNSFFYKTTTKPEAKTTQSANTAVMVQNTQMNNQQTNSYGFTGLSTSSGSMLGAATQQAILDQITKTSLQQYGSQADLKKIIGETKNQLLLDRIANQLIALKPNTSGNSGTQKTIAAYFQTDAVGNPTLDFKAKQKLLLDVLDQYKDFFGNNAQAVQRDSGKSGTGNYLTYTDGSDKITYLQFSYKDIDGLSLSSSNGTSSKFASDVVAALLLFQAADKGTQQLALSSINKPQLPIGDKRIKTGIDLLK", "aa_hexdigest": "00e45c316bfb5cdbf56c8f155aa2e7d5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 530956, "stop": 532287, "observations": { "mutation": [ 532056, 532278 ], "stop": [ 532056, 532278 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75338", "query_cov": 1.0, "subject_cov": 0.4724517906336088, "identity": 0.9940000000000001, "score": 649.0, "evalue": 6.13e-229, "gene_start": 1, "gene_end": 343, "reference_start": 384, "reference_end": 726, "reference_sequence": "MQQQGETKDQYNTFGLRLVRNSVGVSVLGLDGFVKFIKGGSGGSNGGSSSAKKIDKEEQKKFLKFRAFQAKIGTFYNTNFAFSFPLNETLKGWFDKHRGLILANALVKVTLDTKEKASKALVDAFSSYKNWLSEYTPVGLATTMISFYFDQMKALNNKLLERVRSLNQNVNQANPTPWLNGLSAKLPYVNTNGNYEKLNNYFTFLITKVLWPKVGTEDTNVSEEKSKLKTKTEDVNKIREKILNNIDSKLKTFVQKLKPTLAPRPAYSNVILLNINNDKVWSAGANWSLAVLLDPKKVNPLSFMLLKQMFDQNSLFKKAKTLFENIQNKAKTSGSGKSGTTTNDDADALSKVIGNYYYNTWAKLTDKSIYGNLKDDKFDDLFKLAFDSSINEKSFNVDYKAVIEHYRFIYTLEWLVDKNLKNFKDLLKANLKFGEIAFIAYKNTETQNFSNPQGIFGSYFNYENETNAAKSATQIIDPNSFFYKTTTKPEAKTTQSANTAVMVQNTQMNNQQTNSYGFTGLSTSSGSMLGAATQQAILDQITKTSLQQYGSQADLKKIIGETKNQLLLDRIANQLIALKPNTSGNSGTQKTIAAYFQTDAVGNPTLDFKAKQKLLLDVLDQYKDFFGNNAQAVQRDSGKSGTGNYLTYTDGSDKITYLQFSYKDIDGLSLSSSNGTSSKFASDVVAALLLFQAAYKGTQQLALSSINKPQLPIGDKRIKTGIDLLK" }, "paralog": false, "identity": 0.9909706546275395, "score": 855.0, "evalue": 1.71e-312, "description": "Internal stop codon at 532056, 532278. Nonsense mutation around 532056, 532278.", "psc": { "uniref90_id": "UniRef90_P75338", "product": "Uncharacterized protein MPN_440", "uniref50_id": "UniRef50_P75338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75338", "UniRef:UniRef50_P75338" ] }, "pscc": { "uniref50_id": "UniRef50_P75338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75338" ], "product": "Uncharacterized protein MPN_440" } }, "id": "CNOCLNGIAL_957", "locus": "GCA000143945_00942" }, { "type": "cds", "sequence": "contig_1", "start": 532054, "stop": 532272, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGTTTTACTAGATCCGAAAAAGGTTAACCCGCTTTCGTTTATGCTGCTCAAACAAATGTTTGATCAAAACAGTTTGTTTAAAAAAGCAAAGACTTTATTCGAAAACATTCAAAATAAAGCAAAAACTAGTGGAAGTGGTAAAAGTGGTACAACCACCAACGATGATGCCGATGCTTTGAGCAAAGTCATTGGCAACTATTACTACAACACTTGA", "aa": "MAVLLDPKKVNPLSFMLLKQMFDQNSLFKKAKTLFENIQNKAKTSGSGKSGTTTNDDADALSKVIGNYYYNT", "aa_hexdigest": "db33fac5edd172cc0438b4f386071f20", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7995.079200000001, "isoelectric_point": 9.477771186828615 }, "id": "CNOCLNGIAL_958", "locus": "GCA000143945_00943" }, { "type": "cds", "sequence": "contig_1", "start": 532276, "stop": 532497, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCACTGAGGATACTAATGTCAGTGAAGAAAAAAGCAAACTCAAAACTAAAACTGAAGATGTTAACAAGATTAGGGAAAAGATTTTGAACAACATCGACAGTAAGCTCAAAACTTTTGTCCAAAAACTCAAACCTACCTTAGCACCCCGACCAGCTTACAGTAACGTAATCTTGTTAAACATTAACAATGATAAGGTGTGGTCTGCTGGCGCTAATTGA", "aa": "MGTEDTNVSEEKSKLKTKTEDVNKIREKILNNIDSKLKTFVQKLKPTLAPRPAYSNVILLNINNDKVWSAGAN", "aa_hexdigest": "ffd08ece77589e5777c47a77399b7a75", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8214.322999999999, "isoelectric_point": 9.522189903259278 }, "id": "CNOCLNGIAL_959", "locus": "GCA000143945_00944" }, { "type": "cds", "sequence": "contig_1", "start": 532504, "stop": 532743, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTGAATACACCCCGATTGGTTTAGCTACCACCATGATTAGCTTTTATTTTGACCAAATGAAAGCTCTCAACAATAAGCTGTTAGAACGAGTACGGAGCTTAAACCAAAATGTTAACCAAGCCAATCCTACCCCTTGGTTAAATGGGTTATCGGCTAAACTACCTTACGTTAACACTAACGGTAATTACGAAAAGTTAAACAACTACTTTACCTTTTTAATTACCAAAGTTTTGTGA", "aa": "MSEYTPIGLATTMISFYFDQMKALNNKLLERVRSLNQNVNQANPTPWLNGLSAKLPYVNTNGNYEKLNNYFTFLITKVL", "aa_hexdigest": "d0f94f7a39f9b25156f0ab0375b712cd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9092.348699999997, "isoelectric_point": 9.397701454162597 }, "id": "CNOCLNGIAL_960", "locus": "GCA000143945_00945" }, { "type": "cds", "sequence": "contig_1", "start": 532744, "stop": 533136, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAGCAAGGAGAAACTAAAGATCAATATAACACTTTTGGCCTGAGACTTGTGCGTAACAGTGTTGGCGTATCAGTTTTAGGACTTGATGGTTTTGTTAAATTCATAAAAGGCGGTAGCGGAGGTAGCAATGGCGGTTCTAGTAGTGCCAAAAAGATTGATAAAGAAGAGCAAAAGAAATTCTTAAAGTTCCGTGCTTTTCAAGCCAAAATTGGCACATTTTATAACACTAACTTTGCCTTTAGTTTTCCCCTAAACGAAACTTTAAAAGGTTGGTTTGACAAACACCGCGGATTGATCTTAGCGAACGCCTTAGTTAAAGTTACTTTAGACACAAAGGAAAAAGCAAGTAAGGCCTTAGTGGATGCTTTTAGTTCTTATAAAAACTGA", "aa": "MQQQGETKDQYNTFGLRLVRNSVGVSVLGLDGFVKFIKGGSGGSNGGSSSAKKIDKEEQKKFLKFRAFQAKIGTFYNTNFAFSFPLNETLKGWFDKHRGLILANALVKVTLDTKEKASKALVDAFSSYKN", "aa_hexdigest": "526b1f0da89f6d803e10eacb4e5892cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14354.209699999976, "isoelectric_point": 9.910160636901853 }, "id": "CNOCLNGIAL_961", "locus": "GCA000143945_00946" }, { "type": "cds", "sequence": "contig_1", "start": 533143, "stop": 533667, "strand": "-", "frame": 3, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W962", "UniRef:UniRef90_A0AB38W962" ], "nt": "GTGGATGCCACCAACATTGTCGATCAATTAGAGGCAGCTGATTTAGCCGCTGTGCTCAACATTAAACTAGACCGACTGGGTCAAGAGAAGGATGAGCTACTCGAATTTAAGGAGCTCAAAGATGACCTTAACAACGTGATTGTGATTGATAAGAGTAGTGATGCACAAAATGTGAGCAAAACTAATTCTTCTTTTTATGAGGATAATCAAAACTCTGGAACCACAAATACCGATCCCATTGCCCAAGCTTTAGATGACATCTTTGCCACAAACGTGGAACAAAAAACTAATCTAGGCAAGTTAGCAGAACAAATTAAAAAGACAGCGGAAATTAAAACGCAAACCCCACAAGCAAGAGGCACTGTTGTTTTGCGAAATAAATCGCAAACTCAACAAAAGAACAGTTATGTTCTATTAGATGCTGGTATTCCTACCTTTAAACAGCAAGTCAGCACCCAGGGTCAAGGTTCAGTGAGTCTTCATAACGGTACCTTAATTTCTTTAAACACATCTACAGTTGCCTAA", "aa": "MDATNIVDQLEAADLAAVLNIKLDRLGQEKDELLEFKELKDDLNNVIVIDKSSDAQNVSKTNSSFYEDNQNSGTTNTDPIAQALDDIFATNVEQKTNLGKLAEQIKKTAEIKTQTPQARGTVVLRNKSQTQQKNSYVLLDAGIPTFKQQVSTQGQGSVSLHNGTLISLNTSTVA", "aa_hexdigest": "d337888193cbd5a56c12ad59d7dc979e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 533143, "stop": 533912, "observations": { "deletion": [ 533762 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W962", "query_cov": 1.0, "subject_cov": 0.6796875, "identity": 0.9940000000000001, "score": 316.0, "evalue": 2.06e-107, "gene_start": 1, "gene_end": 174, "reference_start": 83, "reference_end": 256, "reference_sequence": "MPFRFLLQRMKLLGVKMSAINAENNWLKVKKRLKKSQSNNKMAVKQKEFKKEGLTKYVRGTDRGQVIDFPLSLSDTNETKKVVDATNIVDQLEAADLAAVLNIKLDRLGQEKDELLEFKELKDDLNNVIVIDKSSDAQNVSKTNSSFYEDNQNSGTTNTDPIAQALDDIFATNVEQKTNLGKLAEQIKKTAEIKTQTPQARGTVVLRNKSQTQQKNSYVLLDAGIPTFKQQVSTQGQGSVSLHNGTLISLNTSTVA" }, "paralog": false, "identity": 1.0, "score": 488.0, "evalue": 1.68e-177, "description": "Frameshift due to deletion around 533762.", "psc": { "uniref90_id": "UniRef90_A0AB38W962", "product": "Protein of unknown function (DUF3713)", "uniref50_id": "UniRef50_A0AB38W962", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W962", "UniRef:UniRef50_A0AB38W962" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W962", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W962" ] } }, "id": "CNOCLNGIAL_962", "locus": "GCA000143945_00947" }, { "type": "cds", "sequence": "contig_1", "start": 533715, "stop": 533864, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGGCAATAAACGCTGAAAACAACTGGTTAAAGGTGAAGAAGCGATTGAAGAAAAGCCAAAGCAACAACAAGATGGCAGTCAAACAAAAGGAGTTCAAAAAGAAGGCCTTACCAAGTATGTACGGGGAACCGACAGAGGCCAAGTAA", "aa": "MSAINAENNWLKVKKRLKKSQSNNKMAVKQKEFKKKALPSMYGEPTEAK", "aa_hexdigest": "bd76df0812bc34d329f092b6a7a7dc4a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5652.6176000000005, "isoelectric_point": 10.202009010314942 }, "id": "CNOCLNGIAL_963", "locus": "GCA000143945_00948" }, { "type": "cds", "sequence": "contig_1", "start": 533845, "stop": 534246, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU13", "UniRef:UniRef90_A0AB33HU13" ], "nt": "TTGTTTGAACAGCTCATTAGCGATTTTGCTAGTCGGGTCTTTCAAAAGAACTACCTCGCTTATAAGGATGGTAATAAGGTATCATCAGGTCCTTTCTTATTTGACGAAATTTCTAAATCGAGCAATTGGGACAAAATTGTCTTTGATGCGGGCCAGTTTCCCGAAATCAATGATGCCTTCTTTGCCCAGATTCAAAGCGAAGTTTTCGATCAATGGGCGGAATACACCGATCCCACTGTTATTAGTTTCACTACCTTAAAGTACTCGGCTCCTAACCAGGGATTAGGGCAAATCTATAACCAGGACAAACTAAAGGATAAATTAACCCCTTCCTATGCCTTTCCGTTTTTTGCTGCAGCGGATGAAATTGCTGGGAGTCAAAATGTCGGCAATAAACGCTGA", "aa": "MFEQLISDFASRVFQKNYLAYKDGNKVSSGPFLFDEISKSSNWDKIVFDAGQFPEINDAFFAQIQSEVFDQWAEYTDPTVISFTTLKYSAPNQGLGQIYNQDKLKDKLTPSYAFPFFAAADEIAGSQNVGNKR", "aa_hexdigest": "bd4544cde609fd15eacd3da6158bab46", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 533684, "stop": 534306, "observations": { "deletion": [ 533763 ], "mutation": [ 533847, 534261 ], "stop": [ 533712, 533847, 534261 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HU13", "query_cov": 1.0, "subject_cov": 0.6394230769230769, "identity": 0.992, "score": 272.0, "evalue": 1.71e-91, "gene_start": 1, "gene_end": 133, "reference_start": 22, "reference_end": 154, "reference_sequence": "MSNKTCWIAPGGGLETWKAQKLFEQLISDFASRVFQKNYLAYKDGNKVSSGPFLFDEISKSSNWDKIVFDAGQFPEINDAFFAQIQSEVFDQWAEYTDPTVISFTTLKYSAPNQGLGQIYNQDKLKDKLTPSYAFPFFAAADEIAGSQNVGNKRWKQLVKGEEAIEEKPKQQQDGSQTKGVQKRRPYQVCTGNRQRPSNWLSVKLIRY" }, "paralog": false, "identity": 0.9759615384615384, "score": 399.0, "evalue": 4.43e-144, "description": "Internal stop codon at 533712, 533847, 534261. Frameshift due to deletion around 533763. Nonsense mutation around 533847, 534261.", "psc": { "uniref90_id": "UniRef90_A0AB33HU13", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AB33HU13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HU13", "UniRef:UniRef50_A0AB33HU13" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HU13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU13" ], "product": "Lipoprotein" } }, "id": "CNOCLNGIAL_964", "locus": "GCA000143945_00949" }, { "type": "cds", "sequence": "contig_1", "start": 534239, "stop": 534439, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_442", "db_xrefs": [ "GO:0098552", "SO:0001217", "UniRef:UniRef50_P75336", "UniRef:UniRef90_P75336" ], "nt": "TTGACAGCTTGGTATGAAAATAACCAAGACAAACAGGTCACCCAGTTCTTTAAAGACACCAAAAAGAGTGTTGATGAGCAATACAACCAAGCCGTAGATAAGGTGGTATCGGCTAGCCGAAACAAAAACCTCTTTGTCCAACAAGACTTGCTGGATAGCGCCGGGGGGGTTAGAAACTTGAAAAGCCCAGAAGTTGTTTGA", "aa": "MTAWYENNQDKQVTQFFKDTKKSVDEQYNQAVDKVVSASRNKNLFVQQDLLDSAGGVRNLKSPEVV", "aa_hexdigest": "5663a320d04936ab63c3a0bb6b06c223", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 534227, "stop": 534679, "observations": { "mutation": [ 534241, 534649 ], "stop": [ 534241, 534649 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75336", "query_cov": 1.0151515151515151, "subject_cov": 0.44370860927152317, "identity": 0.97, "score": 122.0, "evalue": 2.76e-34, "gene_start": 1, "gene_end": 66, "reference_start": 81, "reference_end": 147, "reference_sequence": "MKKLLIKPQFWFLTLGGFISSSVILVACATPSNSALQTVFKARSNQFFNGEQGSLQNALATALKDPEANKQFVAAPLLKALTAWYENNQDKQVTQFFKDTKKSVDEQYNQAVDKVVSASRNKNLFVQQDLLDSAGGGVRNLKSPEVVWTAH" }, "paralog": false, "identity": 0.9801324503311258, "score": 282.0, "evalue": 1.23e-99, "description": "Internal stop codon at 534241, 534649. Nonsense mutation around 534241, 534649.", "psc": { "uniref90_id": "UniRef90_P75336", "product": "Uncharacterized lipoprotein MPN_442", "uniref50_id": "UniRef50_P75336", "go_ids": [ "GO:0098552" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75336", "UniRef:UniRef50_P75336", "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "product": "Uncharacterized lipoprotein MPN_442" } }, "id": "CNOCLNGIAL_965", "locus": "GCA000143945_00950" }, { "type": "cds", "sequence": "contig_1", "start": 534676, "stop": 535008, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAGCTTTTACATCCACCGTTCTGGTAGAGTAGCGCGTTTTAACAGTTGGGGCAATTCCTATGTGTTTGATTTACCGCATAACCAACACAAATTAACTAAGTTTGCCCATAAAGGTATTTTGTTTAACAGTGTTCATTTAGAACGTGATGGTACTTTAAAGTTCCCCCAACTTAAACAAAGCAAACAAAAACCAGCAGTATCCAGTGAACTTAAAACTGCCATTAAGCGGATTAAGGCAGGATACAAGAAGGTCAAGCCTAACTATAAGAAGCGCCAAAAGCAGCAGATTGCGGAACTCTTTGCCAAGCGCAAACAACGCCGTTCATGA", "aa": "MDSFYIHRSGRVARFNSWGNSYVFDLPHNQHKLTKFAHKGILFNSVHLERDGTLKFPQLKQSKQKPAVSSELKTAIKRIKAGYKKVKPNYKKRQKQQIAELFAKRKQRRS", "aa_hexdigest": "78f6aee323532ab0e972ed95b1855ea9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12969.92439999998, "isoelectric_point": 10.869127845764158 }, "id": "CNOCLNGIAL_966", "locus": "GCA000143945_00951" }, { "type": "cds", "sequence": "contig_1", "start": 535021, "stop": 535323, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37" ], "nt": "GTGAAACACTTTCTGCTTAACCTCAACGGTCAAGAACCGTTTGCTGGTTTATTAGCTTTACTCAACTACCACCATAACGAACAGGTCTTGGTCTTTTGTTCCAACGCTAAATCACTCAAACAACTCACTAGTTTACTAGCCCACAACCAAGTTAGCTTTAAAAGCCTCCATGGTCAACTAACGCCCAATGAGCGCAAACACATCTTTACTAGTGCCGCTAATAACACGGTGAGGGTACTAGTGGTATCTGACTTACTAGCACGCGGCATTGATCTGCCCCACTTTAGTGTGGTCATTTCATGA", "aa": "MKHFLLNLNGQEPFAGLLALLNYHHNEQVLVFCSNAKSLKQLTSLLAHNQVSFKSLHGQLTPNERKHIFTSAANNTVRVLVVSDLLARGIDLPHFSVVIS", "aa_hexdigest": "b110d01ea686b7a5cb8275d2b90feb1a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 83, "aa_cov": 0.83, "hmm_cov": 1.0, "evalue": 1.992245133168934e-19, "score": 65.38690185546875, "start": 17, "stop": 99 } ], "seq_stats": { "molecular_weight": 11126.746099999988, "isoelectric_point": 9.428775215148928 }, "id": "CNOCLNGIAL_967", "locus": "GCA000143945_00952" }, { "type": "cds", "sequence": "contig_1", "start": 535336, "stop": 535791, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "GTGGTTGGCATAGCCGAAACTGGTAGTGGTAAAACATTCGCTTACCTCTTACCAGCGCTCAACCAAATTGATGTCACGCTCAACAAGCCCCAAGCGGTGGTGTTTGTACCAACTAAGGAGTTGAAGTGGCAAATTTTAACGATCCTCAACGACATTAAAGCCAGCTTTACTGGTTTAAAGGTCAGTGAGAACTTCAACAGTAATGCCCACCTGATTGTGAGCTTAATTGGCAAAGACATTATTCTCCACGAAACGATTAAGTACGTTGTCTTTGATGAGGTCGACATGTTTTTGGAGGACAGTTCCCAAGCAGAGTGGATTAACTGTGTCCAACTGTTTCAAAAACATAAACCTCACTTTGGCTTTTTTAGTGCCACGTTATTTAACGAACAGCTCCAGCAAATTCGCAAGCAAGTCGCACCTTTAACGGTCGTCAACCAAAAGAAACGTGCTTGA", "aa": "MVGIAETGSGKTFAYLLPALNQIDVTLNKPQAVVFVPTKELKWQILTILNDIKASFTGLKVSENFNSNAHLIVSLIGKDIILHETIKYVVFDEVDMFLEDSSQAEWINCVQLFQKHKPHFGFFSATLFNEQLQQIRKQVAPLTVVNQKKRA", "aa_hexdigest": "eb333483a33dc08916a189b699169f10", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 152, "aa_cov": 0.8874172185430463, "hmm_cov": 0.9868421052631579, "evalue": 1.6093440140744675e-25, "score": 85.22837829589844, "start": 1, "stop": 134 } ], "seq_stats": { "molecular_weight": 17089.640799999994, "isoelectric_point": 7.862386894226074 }, "id": "CNOCLNGIAL_968", "locus": "GCA000143945_00953" }, { "type": "cds", "sequence": "contig_1", "start": 535900, "stop": 536919, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACCGGGCTGCTGATGACACTTCAAGCTTCTTCTACACTAAGTCTAGTAGTAGTACTGGCGCAGCACAAAGTAGTGCCACTGTTTTAAGGAGTTTAAATCAAGCTAGTGGCATGACCACAAAAACTGCCAAAAACCGCTATGGCTTTAGGGGTATTGTAACGAGTAGCACTAGTGGTAGTTTACCCGAAGCGGTAAGTAGACGTTTGTTTAAGCAGTTTGTTAACCAAACAGAAAAAGGCGTTAAAGTTGGTGGTCAAATGCTAATTACCACAGCTAAAAGTGGTAAAGCTACCTTAGTTTTAAAACAACAAGCTGACGACGCCGAATCCACCACCAACAACGCTTACAAAGGTGCCTTGTTTAGCTTTGGTTCGATGGATAACTTAAAGAACATCATCAACGGAATCCAAACGCAAACCGAGTTCGATGCGCTTTACAACCACTTAACTAGTGACCTCAACATTGATGTTACTGGGGTTGATAAAAACAAAACTTTAACGGAACAGAAAACTAGTTTAACGAGTTTTGTTGATTCTAACTTTAAGCAGAGTACTCAATCTGCACAGAGAGGCGATACAAGTGCACGCAGTGCAAGATCTGCAACAGTACAAATTAAGAAAACACAAGAAGATAACCAAAATACCAACTATAAAGACGTCTTTAGCCGCTTTGATGGTTACATTGGTGATAACAAGGTAGAGGAAAAGAACTACACTTCCTACCAATTCTTAAGTGACGGTGGCAAATACCACGCTACCTTTGTCAAACAGGTCAACCTTGATGATGTGGAGAAGATCGGCACTGATTCTCTAAAGCAAGAAGATTCCAGCAAAGACAAACGCCTCAACCTCTCCCTCGAAGAATTCCTCGCTGCAATTGCGCTAGAAGCCTTGGATCCTAATAATCAAACCCAAGCCATTAACGCCTTAATTAGTGGTAATAAGAAGGGTTTAGTTAAAGTAGGTGACTTTAGGATCTTTAGTTCTATTAGTGCGCAATGGGTAAGACGTTTTTAA", "aa": "MNRAADDTSSFFYTKSSSSTGAAQSSATVLRSLNQASGMTTKTAKNRYGFRGIVTSSTSGSLPEAVSRRLFKQFVNQTEKGVKVGGQMLITTAKSGKATLVLKQQADDAESTTNNAYKGALFSFGSMDNLKNIINGIQTQTEFDALYNHLTSDLNIDVTGVDKNKTLTEQKTSLTSFVDSNFKQSTQSAQRGDTSARSARSATVQIKKTQEDNQNTNYKDVFSRFDGYIGDNKVEEKNYTSYQFLSDGGKYHATFVKQVNLDDVEKIGTDSLKQEDSSKDKRLNLSLEEFLAAIALEALDPNNQTQAINALISGNKKGLVKVGDFRIFSSISAQWVRRF", "aa_hexdigest": "7c26ad8d8078b8382ec0416d93a10d3b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37203.80570000003, "isoelectric_point": 9.271343421936034 }, "id": "CNOCLNGIAL_969", "locus": "GCA000143945_00954" }, { "type": "cds", "sequence": "contig_1", "start": 536941, "stop": 537150, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAGCGAAAAGAATAAAAATGATTCACAATCAACTTTATCCAGTACCGCTTCAAGCACAAGTAATACTGGCTTAATTCAGTTAAGATCAGTCGTTTCCCTAGCACAAAATCAAGCAGCTGGCCAAGGCGGTGATAATAATAGTGACATCACCCAAACCGAAGTCAAAAACCCGAACTTTGTGTTTGGTTCGAGTGTGTATGATTGA", "aa": "MDSEKNKNDSQSTLSSTASSTSNTGLIQLRSVVSLAQNQAAGQGGDNNSDITQTEVKNPNFVFGSSVYD", "aa_hexdigest": "c53e9fdcbed1ac14f7763e28c1f4e525", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7242.607900000003, "isoelectric_point": 4.4446615219116214 }, "id": "CNOCLNGIAL_970", "locus": "GCA000143945_00955" }, { "type": "cds", "sequence": "contig_1", "start": 537154, "stop": 537423, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACTTATTTGAATCACAAGCCCAAAAATCAATCTTTGGTCATGTTGGTTCATCTGATAAAAATTCTTCAACTAAGACGGGCATCGAAGGTGTCTTGGACACACTGTACAGCTCGCTAAACTTAGAAGAGCGCTTAGATAGTGATGATGTAATTGATTATCTGTCTTACCTCTACACGGCCCATTGGTTGTTAAAGGACAACTTAAAGAACTATAAGCAAAGCTTACAGTCGAAGTTGTCGCGTACTTCCAATGCCTTTTTAGTTTGA", "aa": "MDLFESQAQKSIFGHVGSSDKNSSTKTGIEGVLDTLYSSLNLEERLDSDDVIDYLSYLYTAHWLLKDNLKNYKQSLQSKLSRTSNAFLV", "aa_hexdigest": "a4d773122e2f7ea61da954a7f99a45c4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10083.09349999999, "isoelectric_point": 5.152136421203611 }, "id": "CNOCLNGIAL_971", "locus": "GCA000143945_00956" }, { "type": "cds", "sequence": "contig_1", "start": 537448, "stop": 539244, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG309-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47551", "UniRef:UniRef90_P75334" ], "nt": "GTGTACGCGGCCATCCAAGACAAACTGTTAGACCAGTTTATTACCAATGAAAACCCCAACTTGGTATCTCGGGTCGTGTTTACCAATGAAACTCCCGTTGACGGGTTTGATAATTACTTTAACACTAAAGTAATTCAAAGTCCAACCCCTTCCTACCAGTTTCAGGTCTTTAACAAATACAACCAACAGAGTGGTGGCACTAAGGGAGCGAATGGCTTTAACTTATTGGCTAGCAACCTCAAGAGTTACAAAAACGACCAGAGCAAAGGGATTGATATTCCCAATAAGTTTTCCAGTGATAGTGGTGGCAAATTGTTGTTAAAGGCGAGCGACATGTTCGACACCTTTGATCCGAGTTTTTCTGCTGCTTTCATTCAGGGCTATTTAGCACTGCAGAAAAAGTCGAAGGGCGCTGATAGTAAAGAAGTTGACAGTCTCATTAAGGATAAGAGTATTATTGAAAACTTTTTTGTTGACAATAATACACAGGCAGCCGCCGCTGCAAGGGCTGCGAGTTCATCATCTGAAGGAACTATTCAATTAAAAACTGCCAGTGATGGTGGTGGAACTACACAAAGTACCGTCCACAAAACTGACTTAGTCAAAATCTTTGGTGATAAAGATGTCTTTGCTGGTGAGTATAAACAACAAATAGGTAATACTAATGCTAATCAAACAGGTGGCGGAGGTTCTGGAGGCGGCGGTGGCACCTCTACCGGCAGTAGCACCGGATCTTCTACTGAAACTACTACTGGCAACAGTAGTAAGGCGGTTGTTGACTTAATTGAAGTTAAAAAAGATTCTAGTAGTCAACCAGACTACATCCTCTCCCGTGGTAAAGACGGCATCCACTTAATGGCCGTGGATGGTGGTAGTCATTATCTTACTGAAAGTGGTCGTGATGTCGCTAAACAAAAGGAATTCTTATTGTTCCGGGCTTTGCAAACCAAGTACGGTTTAGTGGATACCGACACCACATACGACTTTAAGTTATTTGATGAGGTCAAAAAGTACTTTGACACTAACCGGATCTTATTCTTATTTGAAGCTTTATTAGATCTTTCTAGTGATACAAACAACAAAGACAACTTCTTAAGCTATCCCCAGTTTAAGAAGTTTGCTGACAGTATTAAGTCAATTGAGAAGGATTTAAAAGAACTTGTTCAAGCCCACTATAAACAAGCAGTCTTTAACGAAACTGCAGTGGCGGAAAACAAGGTTACCTTAAAGCTAGCAGAACGTAACCAACCTTTTATTGACAACGAGCGCAATAACCAGATTGAACAGAACGGTTTGGCAGCTAAGTTACCTTACGAACAAGATGCTAAAACCGGTCACTATAATGACCTTGGTAACTATTACAAGGACATTATTGATAATGTTGATAAAAAAGGAACTAGTACTGTTAAAACAACCTCTTCCAATACAGGACAAACTAAAAACTTCTCTGAAGAAGTAGTTAGCAAGCTTAAAGATAATAAGAAGAAGGTAGAAGAAGCTGCTAAAAAACATGTAGAGGCACTCAAAGTCTTCACCATTCCTTCCCCACTCTACTCCCAGGTAATCTTAGTGCAAACTAAGTTGTCTTTTACCCCTGAAAGTACCTCTTTAGGACTTAACTTAGCTCTAAACAACTATTTAACCAGTACTGAGCTCCAAAACAGCATTAAGCTCAGTTACTTCCAGGAAGATGAAGCGTTTAAAAAGATTATTGATATTACGAATTTAACTTTTAGTCAACAAAGTGGTGGTACTGGTGGCACCAACGGTAACAACAACCTCACCGCTGACAACTGA", "aa": "MYAAIQDKLLDQFITNENPNLVSRVVFTNETPVDGFDNYFNTKVIQSPTPSYQFQVFNKYNQQSGGTKGANGFNLLASNLKSYKNDQSKGIDIPNKFSSDSGGKLLLKASDMFDTFDPSFSAAFIQGYLALQKKSKGADSKEVDSLIKDKSIIENFFVDNNTQAAAAARAASSSSEGTIQLKTASDGGGTTQSTVHKTDLVKIFGDKDVFAGEYKQQIGNTNANQTGGGGSGGGGGTSTGSSTGSSTETTTGNSSKAVVDLIEVKKDSSSQPDYILSRGKDGIHLMAVDGGSHYLTESGRDVAKQKEFLLFRALQTKYGLVDTDTTYDFKLFDEVKKYFDTNRILFLFEALLDLSSDTNNKDNFLSYPQFKKFADSIKSIEKDLKELVQAHYKQAVFNETAVAENKVTLKLAERNQPFIDNERNNQIEQNGLAAKLPYEQDAKTGHYNDLGNYYKDIIDNVDKKGTSTVKTTSSNTGQTKNFSEEVVSKLKDNKKKVEEAAKKHVEALKVFTIPSPLYSQVILVQTKLSFTPESTSLGLNLALNNYLTSTELQNSIKLSYFQEDEAFKKIIDITNLTFSQQSGGTGGTNGNNNLTADN", "aa_hexdigest": "5dd7cba823f94bb33ed375fc6bec80bf", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 537145, "stop": 539544, "observations": { "mutation": [ 537156, 537450, 539454 ], "stop": [ 537156, 537450, 539454 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75334", "query_cov": 1.0, "subject_cov": 0.44165435745937964, "identity": 0.997, "score": 1066.0, "evalue": 0.0, "gene_start": 1, "gene_end": 598, "reference_start": 211, "reference_end": 808, "reference_sequence": "MHTFTRKVKWPFMFTAIGLTFGIVAVACAQPTASTVEGLFKPSSAFADRTDFSLSSILQKSLINRESFNQYLAMRLAPVLRTFYDDNYDTDIKERLNGFTADTDNAFVSQEQNLRNQFRENYLVHLQTDIFDNTGGNQAAWKLRDVNNKIIDDFISRIFAKNFVEYVQDGVGPLTKPTKSLIENTSNFKNIKLQPKFVNKNAKLKINNDAVYAAIQDKLLDQFITNENPNLVSRVVFTNETPVDGFDNYFNTKVIQSPTPSYQFQVFNKYNQQSGGTKGANGFNLLASNLKSYKNDQSKGIDIPNKFSSDSGGKLLLKASDMFDTFDPSFSAAFIQGYLALQKKSKGADSKEVDSLIKDKSIIENFFVDNNTQAAAAARAASSSSEGTIQLKTASDGGGTTQSTVHKTDLVKIFGDKDVFAGEYKQQIGNTNANQTGGGGSGGGGGTSTGSSTGSSTETTTGNSSKAVVDLIEVKKDSSSQPDYILSRGKDGIHLMAVDGGSHYLTEIGRDVAKQKEFLLFRALQTKYGLVDTDTTYDFKLFDEVKKYFDTNRILFLFEALLDLSSDTNNKDNFLSYPQFKKFADSIKSIEKDLKELVQAHYKQAVFNETAVAENKVTLKLAERNQPFIDNERNNQIEQNGLAAKLPYEQDAKTGHYNDLGNYYKDIIDNVDKKGTSTVKTTSSNTGQTKNFSEEVVSKLKDNKKKVEEAAKKHVEALKVFTIPSPLYSQVILVQTKLSFTPESTSLGLNLALNNYLTSTELQNSIKLSYFQEDEAFKKIIDITNLTFSQQSGGTGGTNGNNNLTADNWKIFKETYLLDLFESQAQKSIFGHVGSSDKNSSTKTGIEGVLDTLYSSLNLEERLDSDDVIDYLSYLYTAHWLLKDNLKNYKQSLQSKLSRTSNAFLVWSVDSEKNKNDSQSTLSSTASSTSNTGLIQLRSVVSLAQNQAAGQGGDNNSDITQTEVKNPNFVFGSSVYDWTNSKTPEVNRAADDTSSFFYTKSSSSTGAAQSSATVLRSLNQASGMTTKTAKNRYGFRGIVTSSTSGSLPEAVSRRLFKQFVNQTEKGVKVGGQMLITTAKSGKATLVLKQQADDAESTTNNAYKGALFSFGSMDNLKNIINGIQTQTEFDALYNHLTSDLNIDVTGVDKNKTLTEQKTSLTSFVDSNFKQSTQSAQRGDTSARSARSATVQIKKTQEDNQNTNYKDVFSRFDGYIGDNKVEEKNYTSYQFLSDGGKYHATFVKQVNLDDVEKIGTDSLKQEDSSKDKRLNLSLEEFLAAIALEALDPNNQTQAINALISGNKKGLVKVGDFRIFSSISAQWVRRFLNHYALHSTTPSVLTNEAHCWIFIYSTSCV" }, "paralog": false, "identity": 0.9949937421777222, "score": 1482.0, "evalue": 0.0, "description": "Internal stop codon at 537156, 537450, 539454. Nonsense mutation around 537156, 537450, 539454.", "psc": { "uniref90_id": "UniRef90_P75334", "product": "Uncharacterized lipoprotein MG309 homolog", "uniref50_id": "UniRef50_P47551", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75334", "UniRef:UniRef50_P47551", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47551", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47551" ], "product": "Uncharacterized lipoprotein MG309" } }, "id": "CNOCLNGIAL_972", "locus": "GCA000143945_00957" }, { "type": "cds", "sequence": "contig_1", "start": 539452, "stop": 539874, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATACGTTTACGCGTAAAGTCAAGTGGCCCTTTATGTTCACCGCAATCGGGCTCACTTTTGGTATAGTCGCGGTTGCCTGTGCGCAGCCTACAGCCAGCACTGTGGAGGGGCTTTTTAAGCCCAGTAGTGCTTTTGCTGATCGCACTGACTTTAGTCTTAGCTCGATTCTGCAAAAGTCACTAATTAACCGCGAGAGTTTTAACCAGTATCTAGCAATGCGTTTAGCACCCGTTTTGCGCACCTTTTATGACGACAATTATGATACTGATATTAAAGAGCGCTTAAACGGCTTTACCGCTGACACGGATAATGCGTTTGTCAGTCAAGAACAAAACCTACGGAACCAGTTTCGCGAAAACTATTTAGTGCACCTGCAAACTGATATCTTTGACAACACTGGCGGTAATCAAGCCGCTTGA", "aa": "MHTFTRKVKWPFMFTAIGLTFGIVAVACAQPTASTVEGLFKPSSAFADRTDFSLSSILQKSLINRESFNQYLAMRLAPVLRTFYDDNYDTDIKERLNGFTADTDNAFVSQEQNLRNQFRENYLVHLQTDIFDNTGGNQAA", "aa_hexdigest": "6d4051e664a05ea3df7894393d909b56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15877.619099999978, "isoelectric_point": 5.594173240661621 }, "id": "CNOCLNGIAL_973", "locus": "GCA000143945_00958" }, { "type": "cds", "sequence": "contig_1", "start": 539929, "stop": 540558, "strand": "+", "frame": 1, "gene": "menH", "product": "Putative esterase/lipase 1", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0016020", "GO:0052689", "SO:0001217", "UniRef:UniRef50_P75333", "UniRef:UniRef90_P75333" ], "nt": "ATGCGGCTCGAAATTGAAAACGGCTTGGAATTTGTGTGTGATCCCTTTTTAAATGAGCGCGGCAAAATCCTTTTTTTACACGCCTTTACCGGTAATATCACCAATAAATTAAGCTTTCGCACCCACTTTAAAGATTACAGTTTTTACGGGATTAACTTTCCCGGACACGGTAACAGTGTCATTCACAACCAAAGTGAATTAGACTTTAACTTCTGGATTAAGTTAGTGCAACAGTTCTTTAATAAATACCAATTAAAGAACGTGGTTTTATTTGGTCACTCGATTGGTGGCGGACTAGCGATTGCCCTCACCCAAGTTTTAACTAAGGAACAAATTAAAGGCATTATCCTCGAAGCGCCCCTAAACCCGGGGATTCGCGCTACCCCACCAAGTATTATTAGTGCCTTGGTACCTGACACCAACGAAGACTTTGAAGCGGTACAAAGGGCATTGATCTACAACATTGAACAACGCTTTGGGGCGAACTTTAAGGACTTTTGTGCTAAACAAAAACAGAAGATGATCCAAAAGTATGCACCCTTAAAGGTAATGTTGCAACCCGAACAAGCCGAACAACGGCTCCAGTTAATTGATGCGGCTTTTAAACGGTTAAGTTACCCAACCCTGTGA", "aa": "MRLEIENGLEFVCDPFLNERGKILFLHAFTGNITNKLSFRTHFKDYSFYGINFPGHGNSVIHNQSELDFNFWIKLVQQFFNKYQLKNVVLFGHSIGGGLAIALTQVLTKEQIKGIILEAPLNPGIRATPPSIISALVPDTNEDFEAVQRALIYNIEQRFGANFKDFCAKQKQKMIQKYAPLKVMLQPEQAEQRLQLIDAAFKRLSYPTL", "aa_hexdigest": "e8ccc20fedc624d2e571c0e4234552d9", "start_type": "ATG", "rbs_motif": null, "genes": [ "menH" ], "truncated": "both", "pseudogene": { "start": 539869, "stop": 540738, "observations": { "mutation": [ 539920, 540556 ], "stop": [ 539920, 540556 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75333", "query_cov": 1.0, "subject_cov": 0.7231833910034602, "identity": 1.0, "score": 414.0, "evalue": 2.81e-145, "gene_start": 1, "gene_end": 209, "reference_start": 21, "reference_end": 229, "reference_sequence": "MHSRSKFGIILKFNHFLWPGMRLEIENGLEFVCDPFLNERGKILFLHAFTGNITNKLSFRTHFKDYSFYGINFPGHGNSVIHNQSELDFNFWIKLVQQFFNKYQLKNVVLFGHSIGGGLAIALTQVLTKEQIKGIILEAPLNPGIRATPPSIISALVPDTNEDFEAVQRALIYNIEQRFGANFKDFCAKQKQKMIQKYAPLKVMLQPEQAEQRLQLIDAAFKRLSYPTLWIHGQEDGIVRYLPSKAYLESLHNPLIELVGLSNTAHTTFFEQPQQFLQLVEQFLNKLNK" }, "paralog": false, "identity": 0.9930795847750865, "score": 577.0, "evalue": 1.47e-211, "description": "Internal stop codon at 539920, 540556. Nonsense mutation around 539920, 540556.", "psc": { "uniref90_id": "UniRef90_P75333", "gene": "MenH", "product": "Putative esterase/lipase 1", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75333", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0016020", "GO:0052689" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75333", "EC:3.1.-.-", "UniRef:UniRef50_P75333", "COG:COG0596", "COG:HR", "GO:0016020", "GO:0052689" ] }, "pscc": { "uniref50_id": "UniRef50_P75333", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75333" ], "product": "Putative esterase/lipase 1" } }, "id": "CNOCLNGIAL_974", "locus": "GCA000143945_00959" }, { "type": "cds", "sequence": "contig_1", "start": 540592, "stop": 540738, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCTCGAAGGCTTACCTGGAATCCTTGCACAATCCTTTAATTGAACTAGTGGGCTTATCCAACACGGCCCACACCACTTTCTTTGAGCAGCCCCAGCAGTTTCTCCAACTAGTGGAACAGTTTTTAAACAAGTTAAACAAATAA", "aa": "MPSKAYLESLHNPLIELVGLSNTAHTTFFEQPQQFLQLVEQFLNKLNK", "aa_hexdigest": "9aec1057b88e71a1af9acc00c25d6d9b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5558.321899999999, "isoelectric_point": 5.99926471710205 }, "id": "CNOCLNGIAL_975", "locus": "GCA000143945_00960" }, { "type": "cds", "sequence": "contig_1", "start": 540799, "stop": 541416, "strand": "-", "frame": 3, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274", "KEGG:K02986", "RefSeq:WP_010874802.1", "SO:0001217", "UniParc:UPI000013500D", "UniRef:UniRef100_P46775", "UniRef:UniRef50_P46775", "UniRef:UniRef90_P46775" ], "nt": "ATGAAATACACTGGTAGTATTTTCAAACGATCACGCCGCTTAGGTTTTTCGTTACTAGAAAACAACAAGGAGTTTTCGAAAGGGAAGAAGCGGAAAACGATTCCTGGACAACACGGGAACCGTTTCCGTAGTTCCACTATGTCTGGTTATGCACAACAGTTGCAAGAAAAGCAACGGATGCAATACATGTATGGGATTACTGATAAACAGTTTAGACGCTTGTTTAGACTGGTATTAAAACAACGGGGTAACTTAGCGGTTAACCTGTTCCGTGTTTTAGAGTCACGTTTAGACAACATTGTGTACCGCATGGGTTTTGCTCCAACCCGTCGCAGTGCGCGCCAATTAGTTAACCACGGCCACGTATTACTCAACGATCGTACCGTGGACACCCCTTCCATTATTCTCAATCCAGGGGATAAGGTACGCTTGAAGGCCAAAACAATTAAGATTCCAATTGTTAAGGCGGCGAGCGAAAGCGGTGTGGTCTCCCCGTTTGTGGAAACCAACAACAAGACCTTTGAAGGGACATACGTGCGCTTCCCAGAACGTAGTGAACTCCCTGCTGGTATTAACGAATCGTACGTGGTCGAATGGTACAAGCGTTTGGTTAAGTAG", "aa": "MKYTGSIFKRSRRLGFSLLENNKEFSKGKKRKTIPGQHGNRFRSSTMSGYAQQLQEKQRMQYMYGITDKQFRRLFRLVLKQRGNLAVNLFRVLESRLDNIVYRMGFAPTRRSARQLVNHGHVLLNDRTVDTPSIILNPGDKVRLKAKTIKIPIVKAASESGVVSPFVETNNKTFEGTYVRFPERSELPAGINESYVVEWYKRLVK", "aa_hexdigest": "8a9fa98fa0470c7da55eccdbcc2f8093", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013500D", "ncbi_nrp_id": "WP_010874802.1", "uniref100_id": "UniRef100_P46775", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013500D", "RefSeq:WP_010874802.1", "UniRef:UniRef100_P46775" ] }, "ips": { "uniref100_id": "UniRef100_P46775", "uniref90_id": "UniRef90_P46775" }, "psc": { "uniref90_id": "UniRef90_P46775", "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_P46775", "cog_id": "COG0522", "cog_category": "J", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274" ] }, "pscc": { "uniref50_id": "UniRef50_P46775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P46775" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "CNOCLNGIAL_976", "locus": "GCA000143945_00961" }, { "type": "cds", "sequence": "contig_1", "start": 541439, "stop": 542443, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAACCAAAGAGGCTTCATCAAATGTTGATGTGGTACAACAACCAGTCAAACCACTCATGCCAGTGATGGTAGAACAGCTCAGAACAACGGAGTTACAGCCAACTACTGAAATTAATCTGTTTGCCAACAGCGACATTAACAGCATTATTGCCGAGTTAAAGCAAGGTCGTTCTAACCCTGCCATTAACTTCGATGACATCTTTAAAATGTCAAGTTACCAAATGGTGGTCAAAAAGTCGTTTGTCCAAATTAGTGACTTTATTACTAATTCCAAAACCGACATTACCAACCGCTTCTTGTTAATCAAAAAGGAATTACAAGCCGAATTGACCCGCTTAATTGAAGAAAACGAACAGCTCAAAGCGGAGTTTTTAAACGCTAAAGACCTTTCGGTGTACCAAAAGGATGAACTGCTACGCTCTTTATCCAACGACTTTACGATTGCTCACCGTCCAAGTGACAGTTACGAACAATTGCAAAAGTCCGGTGAGTTAGTGCGCAACATTCAAAAAGCGATCTTGGAAAACGAAAGCAAGATTAAAAACATCCAGATTACGCTAAAAGAACTTAAAGCCGTTTATAAGCTCTGCAGTGATACTGTTTTAAACGGGATGGCGAAGCTAGACAGTGTGTTGCGCTTTAACAAGAAGGAAAAGGATCCTTTATTGTTAAACAGCATGGAAACATTGTCTAGCTTTGAGACTGAACCGCAAGCCATTATTGAAGATCTCTTGGACTTCTCCAGTAGCTTTGACAAGATGTCCAATGAACAATTGGATGAGTTTGTCTACCAAAACCTAGACAGTGGGTTAAACCTTGATTTAGATGGGTTTGATCATCAGTTAAGCAGCATGAACATCCACGGGTTAGAGCCATTAGATCCAATGAAGTTAGATGACTTTGATTTTGAAACTTTAACTCCAGATAAAACCTCTAACCTCTCTAGTATCTTGGATGATGAACTAATGGAAAACGGTGGGGATTTTAATCTTGATTATTAA", "aa": "METKEASSNVDVVQQPVKPLMPVMVEQLRTTELQPTTEINLFANSDINSIIAELKQGRSNPAINFDDIFKMSSYQMVVKKSFVQISDFITNSKTDITNRFLLIKKELQAELTRLIEENEQLKAEFLNAKDLSVYQKDELLRSLSNDFTIAHRPSDSYEQLQKSGELVRNIQKAILENESKIKNIQITLKELKAVYKLCSDTVLNGMAKLDSVLRFNKKEKDPLLLNSMETLSSFETEPQAIIEDLLDFSSSFDKMSNEQLDEFVYQNLDSGLNLDLDGFDHQLSSMNIHGLEPLDPMKLDDFDFETLTPDKTSNLSSILDDELMENGGDFNLDY", "aa_hexdigest": "0ff80898b9ddaa1a42b984d2a32d594b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38118.626200000086, "isoelectric_point": 4.484448814392089 }, "id": "CNOCLNGIAL_977", "locus": "GCA000143945_00962" }, { "type": "cds", "sequence": "contig_1", "start": 542600, "stop": 543997, "strand": "-", "frame": 2, "gene": "hmw1", "product": "Cytadherence high molecular weight protein 1", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q50365", "UniRef:UniRef90_Q50365" ], "nt": "TTGGAAGCAGATGTACCAGCACCAGAAGTTGCTGAACCTAGCTATGAGGTACAACCAGAGGTAGCTGCTGAGCCTGTTTACGATGTTCAACCAGAAGTAGCTGTAGAACCGGTGGGAGAAACTACTGCAACAGTAGAACCACAAGCAGTAGAAATTCAACCAGAAGTAGTTGTAGAACCAATTGTTGAATCACAACTAGAACAACCTGTGGAAGTTCAAGCTGAAATGGTTCAACCAGAAGTAGCTGTAGAGCCACAATTAGAAGTAAGTCTAGATCCAATTGGAGAAACTGCACCAATATTGGAACAAGTTGAACCACAAGCTGTACAAACCCAACCAGAGATTCCAGCTGAACAATCAGCAGTAGAGCTTCAACCAGAACCAGTTACAGAAGTTCAATCTGAAATGGTTCAACCAGAAGCAGCTGCAGAGCCAGTTACAGAAGCACAACAAACTGAACCAACACCAGTTGTTGAAACTATTGCAGAGATTACACCTCAGGTAGTTACAGAGCCTGTAGTAGCTGTTGTAGAACATCAACCGGAAGCTGTTGCCGAGCCACTTCCAGTAGAACCAGCTGTGGCAGGTGTGTCTGAATTAATTCCAACGGAACAAGTTCAACCAGAAGTGGTTGTGGAATCGACTCCAGTTGCAGAAGTTCAATCTGAAATGGTTCAACCAGAAGTAGCTGTAGAACCGATTGTTGAACCACAACCAGAACAACCTGTGGAAGTCCAACCAGAAGTAATTACAACCCCGGAAGTAGCTTCAGTTTTAGAAGTTCAACCTGAAAATCCTGTTGTAGAAGTGGAACAAGTAGTTGAACCACAACCTGAAACCCCTGTAGAAGTTCAACCAGAACCAGTTGTTGAAACTGTTCAAGAAGCTGTGGCTGAACCAACCCCAGTAGTTGAACCACAACCACAAGCTGCACCTCAACCAGCGGTTTATGAATGGAATCTAACTCCAGAAGCTGCTCCTGTTGAACAACCAGAAGTAATTCCAGTAACAGTAGTAGAAAGCCAAGCTACCGCTACCGCAGAACCTCAACCAGCAGTAGCACCCGTTGCTGATATGGATTATGTCTTACATCTCACCGATACAGTAAAAAACCAACCACAGACTGCACCAGTACAACCAACTACCCCAATTAAAATTGAAGTGGCGGAATCAACTCCTACCGTTACAACCAGTCCAGTTGAACCAACAATTGCCCCACCACTGTTTGAAATTGAACTCAATAATACAACTTCATCTGACTTACCATTAGTTGAGGTTGTGGACTTTAAGCACAATCAACACGGTGCTGTCGGTACCCACAGCTTTGATGATTTTACCCCACCAGAAGTGGGTATGGAAAGCAAGACCCACTGTCACAGTAACAGTGAGGTTGTATGA", "aa": "MEADVPAPEVAEPSYEVQPEVAAEPVYDVQPEVAVEPVGETTATVEPQAVEIQPEVVVEPIVESQLEQPVEVQAEMVQPEVAVEPQLEVSLDPIGETAPILEQVEPQAVQTQPEIPAEQSAVELQPEPVTEVQSEMVQPEAAAEPVTEAQQTEPTPVVETIAEITPQVVTEPVVAVVEHQPEAVAEPLPVEPAVAGVSELIPTEQVQPEVVVESTPVAEVQSEMVQPEVAVEPIVEPQPEQPVEVQPEVITTPEVASVLEVQPENPVVEVEQVVEPQPETPVEVQPEPVVETVQEAVAEPTPVVEPQPQAAPQPAVYEWNLTPEAAPVEQPEVIPVTVVESQATATAEPQPAVAPVADMDYVLHLTDTVKNQPQTAPVQPTTPIKIEVAESTPTVTTSPVEPTIAPPLFEIELNNTTSSDLPLVEVVDFKHNQHGAVGTHSFDDFTPPEVGMESKTHCHSNSEVV", "aa_hexdigest": "309400ab5ee078d4cbf6202b37500909", "start_type": "TTG", "rbs_motif": null, "genes": [ "hmw1" ], "truncated": "both", "pseudogene": { "start": 542297, "stop": 544297, "observations": { "mutation": [ 542602, 544120 ], "stop": [ 542602, 544120 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50365", "query_cov": 1.0, "subject_cov": 0.4567779960707269, "identity": 0.946, "score": 437.0, "evalue": 2.19e-140, "gene_start": 1, "gene_end": 465, "reference_start": 167, "reference_end": 631, "reference_sequence": "MKKSKEAVFEDKDYTEENPEQIFGNLYDGKLTVQDGKVKIAYDGDGNGYYIAFNSETGVYYDPYGDTEYDISVLFDANGNSFVFADAPTVEVLAGEQEQTEAEPDYLQYVGNEAYGYYDEAGEWVWSGYFEGDQWISTLPQTEAEEKQFGFEDNIETTPTASEDFGLEADVPAPEVAEPSYEVQPEVAAEPVYDVQPEVAVEPVGETTATVEPQAVEIQPEVVVEPIVESQLEQPVEVQAEMVQPEVAVEPQLEVSLDPIGETAPILEQVEPQAVQTQPEIPAEQSAVELQPEPVAEVQSEMVQPEAAAEPVTEAQQTEPTPVVETIAEITPQVVTEPVVAVVEHQPEAVAEPLPVEPAVAGVSELIPTEQVQPEVVVESTPVAEVQSEMVQPEVAVEPIVEPQPEQPVEVQPEVITTPEVASVLEVQPENPVVEVEQVVEPQPETPVEVQPEPVVETVQEAVAEPTQVVEPQPQAAPQPAVYEWNLTPEAAPVEQPEVIPVTVVESQATATAEPQPAVAPVADMDYVLHLTDTVKNQPQTAPVQPTTPIKIEVAESTPTVTTSPVEPTIAPPLFEIELNNTTSSDLPLVEVVDFKHNQHGAVGTHSFDDFTPPEVGMESKTHCHSNSEVVWRVSEPKTVPVPPAVSSINIQTVNRVVEPTISTPTTPVVESAPAIEIFVDTPPVETKEASSNVDVVQQPVKPLMPVMVEQLRTTELQPTTEINLFANSDINSIIAELKQGRSNPAINFDDIFKMSSYQMVVKKSFVQISDFITNSKTDITNRFLLIKKELQAELTRLIEENEQLKAEFLNAKDLSVYQKDELLRSLSNDFTIAHRPSDSYEQLQKSGELVRNIQKAILENESKIKNIQITLKELKAVYKLCSDTVLNGMAKLDSVLRFNKKEKDPLLLNSMETLSSFETEPQAIIEDLLDFSSSFDKMSNEQLDEFVYQNLDSGLNLDLDGFDHQLSSMNIHGLEPLDPMKLDDFDFETLTPDKTSNLSSILDDELMENGGDFNLDY" }, "paralog": false, "identity": 0.9594594594594594, "score": 854.0, "evalue": 1.82e-305, "description": "Internal stop codon at 542602, 544120. Nonsense mutation around 542602, 544120.", "psc": { "uniref90_id": "UniRef90_Q50365", "gene": "hmw1", "product": "Cytadherence high molecular weight protein 1", "uniref50_id": "UniRef50_Q50365", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50365", "UniRef:UniRef50_Q50365", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q50365", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50365" ], "product": "Cytadherence high molecular weight protein 1" } }, "id": "CNOCLNGIAL_978", "locus": "GCA000143945_00963" }, { "type": "cds", "sequence": "contig_1", "start": 544118, "stop": 544495, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16713.10" ], "nt": "ATGAAGAAAAGCAAAGAAGCAGTTTTTGAAGACAAGGATTACACTGAGGAAAATCCCGAACAGATCTTTGGTAATTTGTACGATGGTAAATTAACTGTTCAAGATGGCAAAGTCAAGATTGCCTATGACGGTGATGGCAATGGTTATTACATTGCCTTTAACAGTGAAACTGGAGTTTACTATGATCCTTATGGTGACACGGAATACGACATTAGCGTGTTGTTTGACGCCAATGGTAATCCGTTCGTGTTTGCTGATGCACCAACAGTGGAAGTTTTAGCGGGGGAACAGGAACAAACCGAAGCAGAACCGGATTACCTTCAGTATGTCGGTAATGAGGCTTATGGTTACTATGATGAAGCTGGGGAGTGGGTTTGA", "aa": "MKKSKEAVFEDKDYTEENPEQIFGNLYDGKLTVQDGKVKIAYDGDGNGYYIAFNSETGVYYDPYGDTEYDISVLFDANGNPFVFADAPTVEVLAGEQEQTEAEPDYLQYVGNEAYGYYDEAGEWV", "aa_hexdigest": "6d171dae384ca7f0d10fdc5e36556cb8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16713.10", "name": "EAGR_box", "length": 22, "aa_cov": 0.176, "hmm_cov": 1.0, "evalue": 9.370775495972897e-10, "score": 33.555137634277344, "start": 104, "stop": 125 } ], "seq_stats": { "molecular_weight": 14087.910899999999, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_979", "locus": "GCA000143945_00964" }, { "type": "cds", "sequence": "contig_1", "start": 544559, "stop": 544840, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTAAACCTCTACATCTTAATGATGGCCTTACTAATGACCTTAGTGGTATCATTGTTAATTGGCACTATAGCCTCGATTGAATACCTCGTCTTTTTAAGTGGTAAACCAAGTAAGAACTTCGTTAAGTATGGTAGCTATTTCTTTTTAATGCCAAGGGTCTTGGTACAAGCGTTGTTAATGCCCTTGTACTTGGCCTTGTTTAAGCCCTTAATTAGGATTGCTGAAAATAATTTAAGAAATTATTTAAGGGTATACAACCTCTCATGGAAAAGATAA", "aa": "MILNLYILMMALLMTLVVSLLIGTIASIEYLVFLSGKPSKNFVKYGSYFFLMPRVLVQALLMPLYLALFKPLIRIAENNLRNYLRVYNLSWKR", "aa_hexdigest": "74f4eb010f34818aa31f324444d287d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10864.250999999987, "isoelectric_point": 10.136186790466308 }, "id": "CNOCLNGIAL_980", "locus": "GCA000143945_00965" }, { "type": "cds", "sequence": "contig_1", "start": 544871, "stop": 545014, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACCTGACTGATGGCAATTTTCAAGCTGCTGGTAACCAGAGCTTTGGCCGGGGCTTTGATGTCAATGTCCACATCCTACAAGGCATTACGATGGCAGCCTTTATTAGCTTTTTTGTGGGGTTAGAAATCTTTTTGGGTTGA", "aa": "MYLTDGNFQAAGNQSFGRGFDVNVHILQGITMAAFISFFVGLEIFLG", "aa_hexdigest": "bb18af03e57aff4bd5780654a1f755b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5115.793199999997, "isoelectric_point": 4.535092353820801 }, "id": "CNOCLNGIAL_981", "locus": "GCA000143945_00966" }, { "type": "cds", "sequence": "contig_1", "start": 545084, "stop": 545350, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACTAAGCTTTATCTTTAATATTTTTTCCATTAATGTCACTAGTGTGCTCAAGGTGAGTTTCACAAGAATTCCCTTTGCCTTAATTGGTTGGATGTTTGGACCGGTGTGGGGCTTTACCTTTGGCGCCATTGCCGACACGATGGACTGGCTTACTAGGGGCTATACCTGGTTTTGGTTGTTTGCGATTCAAAAACCCATGTTCTGCTTTTTAGCGGGGCTGGTTAAGGGCGTTTATCAGGTACGCCAAAGTAGTACTAACTGA", "aa": "MALSFIFNIFSINVTSVLKVSFTRIPFALIGWMFGPVWGFTFGAIADTMDWLTRGYTWFWLFAIQKPMFCFLAGLVKGVYQVRQSSTN", "aa_hexdigest": "f28142e6e63ca6ca4e1b4291e79cfbc1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10104.854499999996, "isoelectric_point": 9.860197639465333 }, "id": "CNOCLNGIAL_982", "locus": "GCA000143945_00967" }, { "type": "cds", "sequence": "contig_1", "start": 545467, "stop": 546192, "strand": "-", "frame": 3, "gene": null, "product": "MPN449 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363", "UniRef:UniRef90_Q50363" ], "nt": "ATGTTGTTAATTGGAGCGGCCGCCATTGCTTACCTCGTGGTGGTAAACCTCTTGTTTTACAGGTATGTAAACCCTGACAGTAACCTAACTAAAGCGGTTACTGGTCAGAATAATACCGACAGGCCAGCGTTAGTTGATTTAAACGGTGGCATTAACCTCTTTTTCCCTGTTAGTGCTTCTACTTTAATTACCTTGATCTTCTTGGGTTTTGGTAGTACTTCCTTCCTTTTAGCCTTTCAAGGTAAGCCCTACAGTTTTGCTACTAGAAGTCAAACCTTTAAGGCAATGCACTTTCTCAAGCACCAGTTTGGGGTAACTGATTTTCCCAGGATCAATGACAACTACCGGTATAAGGTCAGAATTAAGTGGATCTTCTGGACAATCTTCTTGTTTGTGGCACTCAATGCCCTACCTGGGGGTAACATTATTACAGGTGGTATTTTAAACCCCTCTTTTCTGTTAAATGCCTTAAGGTCTAATGAATTAAAAATTAACAGTGAGACCTTTAAAACTATCTTTGTTGGTTTTTCCATATTTTACCTAGCGAGCATTATTCCCTTTGCCCTCATTAGTATTATTGCCTTTGTTTTGAGTCCTAAACCAAGTTCGGATCAAACAAACGAAGTGTTGAACCGTTACGTGCAAGAGGAAATGCAACAGCCATTTAAAACGGATTGTGATCCTAACAACGACAATGATTTAACACCCCCAGCTGTTTTTGGGTAA", "aa": "MLLIGAAAIAYLVVVNLLFYRYVNPDSNLTKAVTGQNNTDRPALVDLNGGINLFFPVSASTLITLIFLGFGSTSFLLAFQGKPYSFATRSQTFKAMHFLKHQFGVTDFPRINDNYRYKVRIKWIFWTIFLFVALNALPGGNIITGGILNPSFLLNALRSNELKINSETFKTIFVGFSIFYLASIIPFALISIIAFVLSPKPSSDQTNEVLNRYVQEEMQQPFKTDCDPNNDNDLTPPAVFG", "aa_hexdigest": "6d75d32db0a653f77671fa6947da4dca", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 545467, "stop": 546492, "observations": { "mutation": [ 546219, 546228 ], "stop": [ 546219, 546228 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50363", "query_cov": 1.0, "subject_cov": 0.5379464285714286, "identity": 1.0, "score": 472.0, "evalue": 4.01e-165, "gene_start": 1, "gene_end": 241, "reference_start": 208, "reference_end": 448, "reference_sequence": "MTFSDLLTKLQDNLDIVFNANALKERIQSDPAFAKTIREQLKLLYFLEQKQAKAKTKKKDFQPVFQNLEARFVSLGQTKLANTELNLKLDLTDATDLANYLPIAVCNLFNRDLNSFSKLSSVQPTAVEKTTNGANKPTVTIDLNQPRIHTTGTVSPELENFVNESLEARARQRAFMRVTSERMVGKIFEFQFKSQWIKWAQLAIFISMLLIGAAAIAYLVVVNLLFYRYVNPDSNLTKAVTGQNNTDRPALVDLNGGINLFFPVSASTLITLIFLGFGSTSFLLAFQGKPYSFATRSQTFKAMHFLKHQFGVTDFPRINDNYRYKVRIKWIFWTIFLFVALNALPGGNIITGGILNPSFLLNALRSNELKINSETFKTIFVGFSIFYLASIIPFALISIIAFVLSPKPSSDQTNEVLNRYVQEEMQQPFKTDCDPNNDNDLTPPAVFG" }, "paralog": false, "identity": 0.9941348973607038, "score": 667.0, "evalue": 4.33e-244, "description": "Internal stop codon at 546219, 546228. Nonsense mutation around 546219, 546228.", "psc": { "uniref90_id": "UniRef90_Q50363", "product": "MPN449 family protein", "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50363", "UniRef:UniRef50_Q50363" ] }, "pscc": { "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363" ], "product": "Uncharacterized protein MG314 homolog" } }, "id": "CNOCLNGIAL_983", "locus": "GCA000143945_00968" }, { "type": "cds", "sequence": "contig_1", "start": 546226, "stop": 546813, "strand": "-", "frame": 3, "gene": null, "product": "MPN449 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363", "UniRef:UniRef90_Q50363" ], "nt": "ATGACTTTTAGCGATCTTTTAACCAAGTTACAAGACAATTTAGACATTGTTTTTAATGCCAATGCCCTCAAAGAGCGGATCCAAAGCGACCCGGCGTTTGCTAAAACAATTCGCGAACAGTTAAAGCTGTTGTACTTTTTAGAGCAAAAGCAAGCTAAGGCTAAAACCAAGAAAAAGGACTTTCAACCTGTTTTTCAAAACTTAGAAGCGCGCTTTGTTAGTTTAGGGCAAACCAAACTAGCGAACACCGAACTTAACCTCAAACTTGATTTAACTGATGCCACGGACTTGGCTAACTATTTACCAATTGCCGTGTGCAATCTCTTTAACCGTGATTTAAATAGCTTTAGTAAGTTAAGTAGCGTTCAACCAACAGCTGTTGAAAAAACAACCAATGGAGCTAATAAACCGACGGTTACAATTGATTTAAACCAACCACGGATACATACAACTGGTACTGTAAGCCCAGAGTTGGAAAACTTTGTCAACGAATCACTCGAAGCACGTGCACGCCAACGTGCCTTTATGCGGGTCACCTCGGAACGGATGGTAGGCAAAATCTTTGAATTCCAGTTTAAAAGCCAGTGA", "aa": "MTFSDLLTKLQDNLDIVFNANALKERIQSDPAFAKTIREQLKLLYFLEQKQAKAKTKKKDFQPVFQNLEARFVSLGQTKLANTELNLKLDLTDATDLANYLPIAVCNLFNRDLNSFSKLSSVQPTAVEKTTNGANKPTVTIDLNQPRIHTTGTVSPELENFVNESLEARARQRAFMRVTSERMVGKIFEFQFKSQ", "aa_hexdigest": "d7f164fbd3bcdda1858d2b919b7ec8c9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 545923, "stop": 546813, "observations": { "mutation": [ 546219, 546228 ], "stop": [ 546219, 546228 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50363", "query_cov": 1.0, "subject_cov": 0.43526785714285715, "identity": 1.0, "score": 369.0, "evalue": 4.35e-125, "gene_start": 1, "gene_end": 195, "reference_start": 1, "reference_end": 195, "reference_sequence": "MTFSDLLTKLQDNLDIVFNANALKERIQSDPAFAKTIREQLKLLYFLEQKQAKAKTKKKDFQPVFQNLEARFVSLGQTKLANTELNLKLDLTDATDLANYLPIAVCNLFNRDLNSFSKLSSVQPTAVEKTTNGANKPTVTIDLNQPRIHTTGTVSPELENFVNESLEARARQRAFMRVTSERMVGKIFEFQFKSQWIKWAQLAIFISMLLIGAAAIAYLVVVNLLFYRYVNPDSNLTKAVTGQNNTDRPALVDLNGGINLFFPVSASTLITLIFLGFGSTSFLLAFQGKPYSFATRSQTFKAMHFLKHQFGVTDFPRINDNYRYKVRIKWIFWTIFLFVALNALPGGNIITGGILNPSFLLNALRSNELKINSETFKTIFVGFSIFYLASIIPFALISIIAFVLSPKPSSDQTNEVLNRYVQEEMQQPFKTDCDPNNDNDLTPPAVFG" }, "paralog": false, "identity": 0.9932432432432432, "score": 565.0, "evalue": 1.47e-204, "description": "Internal stop codon at 546219, 546228. Nonsense mutation around 546219, 546228.", "psc": { "uniref90_id": "UniRef90_Q50363", "product": "MPN449 family protein", "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50363", "UniRef:UniRef50_Q50363" ] }, "pscc": { "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363" ], "product": "Uncharacterized protein MG314 homolog" } }, "id": "CNOCLNGIAL_984", "locus": "GCA000143945_00969" }, { "type": "cds", "sequence": "contig_1", "start": 547002, "stop": 547724, "strand": "-", "frame": 1, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360", "SO:0001217", "UniRef:UniRef50_Q50362", "UniRef:UniRef90_Q50362" ], "nt": "ATGACCGTAGTTTATGGCGCCGATATTGGGTTAATTCACCAGCAGTTAAACCAACTGCTCAACCCAGCCGCTTGTAAACAGGTCTGGTTTCAAGATGTTAATAAGCTGTATGATGTGGTGTTAAACCAGAATCTGTTTGCTGAGGACACTAAACCGATCCTCATTCACAACTGCAGTTTTTTGGAAAAGAACAACTTAACTAAGGCTGAATTACACTGCTTAAAAACACTTAAAGATACTGATGTGGTGGTCACCATTTACAGCGACAGTCCCGCTAATGCCCTCATTAATGACCGGGCAATTACCAAGTATGCTTGTAAACCAGTCACAGCTAAAACCATCCACCAGGTGATCAGTAAGGCCGCTAAGACACTCAAACTCAACTTAAACCCTGATTTAATTGACCACTTAGCCACGATTCTACCCTTTAACTTAGGGGTCATTGAACAGGAGTTGCGTAAGTTAACTCTACTCAGCCCGGCAGAATTACAGGACAAAAAAATGTTAGAAGCCGTTTTGTGTGATTACCAAACCAGTCAAATCTTACAATTAACTGACGCCATGGTGCGTTTGCAAACAGCTAAAGCCTTAAAGTTGATTGAACGCTTGTTTTTACCAAAGCAGCTCACACCACCGCAATTCCTAGAATTTTTAGCAAATGAACTACTTTTGGCCTTGATGGTCAAAGGCGTTAAACCACAAGCTGTTTTCCAACTACAGTGA", "aa": "MTVVYGADIGLIHQQLNQLLNPAACKQVWFQDVNKLYDVVLNQNLFAEDTKPILIHNCSFLEKNNLTKAELHCLKTLKDTDVVVTIYSDSPANALINDRAITKYACKPVTAKTIHQVISKAAKTLKLNLNPDLIDHLATILPFNLGVIEQELRKLTLLSPAELQDKKMLEAVLCDYQTSQILQLTDAMVRLQTAKALKLIERLFLPKQLTPPQFLEFLANELLLALMVKGVKPQAVFQLQ", "aa_hexdigest": "086fc53dabb39ca3579aac98dda307f0", "start_type": "ATG", "rbs_motif": null, "genes": [ "holA" ], "truncated": "5-prime", "pseudogene": { "start": 546825, "stop": 547769, "observations": { "mutation": [ 546953, 547004 ], "stop": [ 546953, 547004 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50362", "query_cov": 1.0, "subject_cov": 0.7643312101910829, "identity": 1.0, "score": 462.0, "evalue": 4.44e-163, "gene_start": 1, "gene_end": 240, "reference_start": 16, "reference_end": 255, "reference_sequence": "MGCGACCILWVQSFTMTVVYGADIGLIHQQLNQLLNPAACKQVWFQDVNKLYDVVLNQNLFAEDTKPILIHNCSFLEKNNLTKAELHCLKTLKDTDVVVTIYSDSPANALINDRAITKYACKPVTAKTIHQVISKAAKTLKLNLNPDLIDHLATILPFNLGVIEQELRKLTLLSPAELQDKKMLEAVLCDYQTSQILQLTDAMVRLQTAKALKLIERLFLPKQLTPPQFLEFLANELLLALMVKGVKPQAVFQLQWNVNPFRLKAIQSQYRFWSTNQLTALINAIWQLDIKIKRNDGLAIHLLKHFVLRFFAQK" }, "paralog": false, "identity": 0.9936305732484076, "score": 615.0, "evalue": 1.03e-225, "description": "Internal stop codon at 546953, 547004. Nonsense mutation around 546953, 547004.", "psc": { "uniref90_id": "UniRef90_Q50362", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q50362", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50362", "EC:2.7.7.7", "UniRef:UniRef50_Q50362", "COG:COG1466", "COG:L", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_Q50362", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50362" ], "product": "Probable DNA polymerase III subunit delta" } }, "id": "CNOCLNGIAL_985", "locus": "GCA000143945_00970" }, { "type": "cds", "sequence": "contig_1", "start": 547912, "stop": 548046, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACTTCCACCTTAATCCTCATTGTGGTCAGTCCGATTACTTTGCACTTAAATCGAAAATTAAACGCTTTAAGTGTGTTTCATAACCTCTTGTTTACTCCCATAGCCTTGTTTTACTTCTGTGCTAGCTGA", "aa": "MLTSTLILIVVSPITLHLNRKLNALSVFHNLLFTPIALFYFCAS", "aa_hexdigest": "c80a687094b032b51a396136492493e0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4946.955299999999, "isoelectric_point": 9.304415702819824 }, "id": "CNOCLNGIAL_986", "locus": "GCA000143945_00971" }, { "type": "cds", "sequence": "contig_1", "start": 548062, "stop": 548382, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAGTGGTTTTCACCTTAGTTTTTTGTTTGGTGTCATGGAGCGCTGGTTGTACAAGCGCTTTTACATCAATAAAATAACGGGTTTTGTAATGCTGTTGCTGTACCTCTTTTTGGTCGGATTTGCCTTTTCAGCTTTACGGGTTTTTGTCTCAAAGCTGTTACGGCAAATGTTTCCCAAGCAACTACCCGAAAATAATTTGGGCTTAACGGCGCTGTTAATCATCTTAATGAGTCCGGGAGCGTTACACAACTTTGGCTTTAACTTTTCCTTTTTAGCGTGCTTTGTGTTGTTTGCCATTAATAAGGTGAAGCTGTGA", "aa": "MISGFHLSFLFGVMERWLYKRFYINKITGFVMLLLYLFLVGFAFSALRVFVSKLLRQMFPKQLPENNLGLTALLIILMSPGALHNFGFNFSFLACFVLFAINKVKL", "aa_hexdigest": "0a5e240b53b4d9b5b8e822d756a33c53", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12265.821599999981, "isoelectric_point": 10.294843482971189 }, "id": "CNOCLNGIAL_987", "locus": "GCA000143945_00972" }, { "type": "cds", "sequence": "contig_1", "start": 548524, "stop": 548658, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAGTTAACCTAATTAGTTTAACGGTACACTGGAAGTTTGGCTTACTGTTAATCAGTTGTAGTGCCGCTTTACTAGCATACTGTCAGTTAATGCCAGAGTTTCAACTAGCCAAACTAATGAAAGCGAACTGA", "aa": "MIVNLISLTVHWKFGLLLISCSAALLAYCQLMPEFQLAKLMKAN", "aa_hexdigest": "ac259c2c66f82dcac581e709e9b4dad9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4938.056199999999, "isoelectric_point": 8.803689765930176 }, "id": "CNOCLNGIAL_988", "locus": "GCA000143945_00973" }, { "type": "cds", "sequence": "contig_1", "start": 548797, "stop": 549063, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGTAAACTACCGCAACCCAGCGAGTTTGAACTTCGACAGCCTTAACAGCTTTGGCGCTTACCGCAGTCCAAGTAGCTTTTACCCACTTAGAAGACCATTGGAATTGTCGAGCTTAAGGCGTAACCGCAGTTCCTTTTTCAACACGCACCGCTTTGATTTAGGGAGCAATTACACCAGTTTCACCCCGCGTTACCGCTCACCGTTACGTGGTGGTTTGTCTCAACGCTTTCCGCTCCGTTCCAGCTGGAGTAAAGAGTTTTAA", "aa": "MAVNYRNPASLNFDSLNSFGAYRSPSSFYPLRRPLELSSLRRNRSSFFNTHRFDLGSNYTSFTPRYRSPLRGGLSQRFPLRSSWSKEF", "aa_hexdigest": "b4490d7ee4ccee62d3db0bd631e5022c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10322.40539999999, "isoelectric_point": 11.510265922546388 }, "id": "CNOCLNGIAL_989", "locus": "GCA000143945_00974" }, { "type": "cds", "sequence": "contig_1", "start": 549130, "stop": 549366, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAACCACAACCGTGTCAGTCAACTTGTGTTAATAATGAAAGTGGTGCAATTACCATTCACACCACCAACCGTTCCTTATTGTTGGAAAAGCTAGCGAGTTTGGGTCACCTCCACGATGCTTCTACCCGTACGCCTTTACCGCACGAGCGCTACCAGTTAGCTCCACCGAGTGAATATGTGGCTACCAAGTACAACGAACCGCTGTTTAACCTTCCAGCAATCCGCAACAGTTGA", "aa": "MEPQPCQSTCVNNESGAITIHTTNRSLLLEKLASLGHLHDASTRTPLPHERYQLAPPSEYVATKYNEPLFNLPAIRNS", "aa_hexdigest": "0ffd67c7474e9ae00ab90b044496e46d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8684.680499999995, "isoelectric_point": 6.386338233947753 }, "id": "CNOCLNGIAL_990", "locus": "GCA000143945_00975" }, { "type": "cds", "sequence": "contig_1", "start": 549388, "stop": 550815, "strand": "-", "frame": 3, "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q50360", "UniRef:UniRef90_Q50360" ], "nt": "ATGACAGATAAAGAAAGAGCAAAGCTAGCGAAAGCGTATGGTAAGCTAGCACAAAAGATCCAAAAGAGCTATCCCGACATTAATGTGGTTTATGGTCGTGATGCTAAAAATAAGCTTCACGCCCTGTACCAGGATCCTGAAACCGGGAACATCTTTTCTTTGGAAAAAAGAAAACAGCTCCCAGCGGATTATCCTCTGTTCGAATTGGACAGTGATGAACCAATTAGCTTTGCCCCCAAGATTATTCCACTCACTGCCTTTGATGGCAACAACAATGAGGTAATAGTCCAATACGACCAGGTCAACAATACCTTTTACGACCAAGATGGCAATGTTTTGGATGTGAGTGGTTACCGTGATGGGGAAAACATTCCCCTAGTGGACTATTTAAACTATGGTGGTAGTACCGCAAGTGCAGATACCACTACAAGCGAACCATTGAGTGGTGAGGGCTATCCGGACATTGATGCTGGTTTACCAGTTGTGGATCCCGATGCTACACCCGAACAGCAAGCTGATCAACTGTTCGGACTCGATCCACTACCTCAAGCACCAGACGAATATCAGGATACTACAGCACCACCAGTTTATGATCAGACCTTTGATCAAGCAACATACGACCAGCAAGCGTATGACCAAAACTATGATCCGAACGCTTACTATGACCAGCAAGCTTACGATCAGTCCTTTGATCAACAAGCGTATGATCAAGCCTATGATGCTAATGCTTACAATACACAGAACTATGATCAAGCTCACGATCCGAACGCTTACTATGATTCCCAAGCTTACAGCGATCCTGATCAAGCTAGTGCTGTAGCACCAATAGAGGTAGCACCACTCCAACCAGAACCAGTTGCACCAGTAGTGGAGCCAACTGCTGTTCCTATTGTTGAATCTGCTCCAATAGTAGAGGTTACTCCAACAGTAGAACCTACCCCAACACCAGTGGTAGAGACCGCACCAGTAGTGGAAGCTCCTAAGGTAGTGGAACCTACCCCAACACCAGTGGTAGAAGCTACTCCTGCCCCTAAAGTAGAGCCTAAGGTAGTGGAACAACCACAACCAACACCTGTAACTGTTGAGGTTGACTCACCTAAAGTTGAAATACCGAAGGTAGTAACAGCAAAAGTTGCACTCCAAGTGGCCCAACCAACCCCTGTTCCAGCTGTACCTAAAGTAGCACCACAACCAACCCCTGCTCCAGTAGTTGTCCAACCAACTGCTGTAGTTCAACCAGTTGTTAAGGCAGAACCGAAGGTAGTAACACCAACGCCAGCACCACAGGTAGTAGTTACTCCTCAAGTAGCTACACCTAAAGTGACACCTAAGGTAGTCCAAACAACTCCTGCGGTTCCACCAGTTGTAGTTCAACCAGAAGTGGTAGTCCAACCAATTATCCGTCCAACACAACCCGAACCGGAATGA", "aa": "MTDKERAKLAKAYGKLAQKIQKSYPDINVVYGRDAKNKLHALYQDPETGNIFSLEKRKQLPADYPLFELDSDEPISFAPKIIPLTAFDGNNNEVIVQYDQVNNTFYDQDGNVLDVSGYRDGENIPLVDYLNYGGSTASADTTTSEPLSGEGYPDIDAGLPVVDPDATPEQQADQLFGLDPLPQAPDEYQDTTAPPVYDQTFDQATYDQQAYDQNYDPNAYYDQQAYDQSFDQQAYDQAYDANAYNTQNYDQAHDPNAYYDSQAYSDPDQASAVAPIEVAPLQPEPVAPVVEPTAVPIVESAPIVEVTPTVEPTPTPVVETAPVVEAPKVVEPTPTPVVEATPAPKVEPKVVEQPQPTPVTVEVDSPKVEIPKVVTAKVALQVAQPTPVPAVPKVAPQPTPAPVVVQPTAVVQPVVKAEPKVVTPTPAPQVVVTPQVATPKVTPKVVQTTPAVPPVVVQPEVVVQPIIRPTQPEPE", "aa_hexdigest": "8d6e8339324baec003de8715b3518925", "start_type": "ATG", "rbs_motif": null, "genes": [ "hmw3" ], "truncated": "5-prime", "pseudogene": { "start": 549085, "stop": 550815, "observations": { "mutation": [ 549132, 549390 ], "stop": [ 549132, 549390 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50360", "query_cov": 0.9136842105263158, "subject_cov": 0.6439169139465876, "identity": 0.965, "score": 547.0, "evalue": 1.83e-187, "gene_start": 1, "gene_end": 432, "reference_start": 1, "reference_end": 434, "reference_sequence": "MTDKERAKLAKAYGKLAQKIQKSYPDINVVYGRDAKNKLHALYQDPETGNIFSLEKRKQLPADYPLFELDSDEPISFAPKIIPLTAFDGNNNEVIVQYDQVNNTFYDQDGNVLDVSGYRDGENIPLVDYLNYGGSTASADTTTSEPLSGEGYPDIDAGLPVVDPDATPEQQADQLFGLDPLPQAPDEYQDTTAPPAYDQTFDQATYDQQAYDQNYDPNAYYDQQAYDQSFDQQAYDQAYDANAYNTQNYDQAHDPNAYYDSQAYSDPDQAXXSAVAPIEVAPLQPEPVAPVVEPTAVPIVESAPIVEVTPTVEPTPTPVVETAPVVEAPKVVEPTPTPVVEATPAPKVEPKVVEQPQPTPVTVEVDSPKVEIPKVVTAKVALQVAQPTPVPAVPKVAPQPTPAPVVVQPTAVVQPVVKAEPKVVTPTPAPQVVVTPQVATPKVTPKVVQTTPAVPPVVVQPEVVVQPIIRPTQPEPEWKPSPASVVEPQPCQSTCVNNESGAITIHTTNRSLLLEKLASLGHLHDASTRTPLPHERYQLAPPSEYVATKYNEPLFNLPAIRNSWARFTRPTVESTPIASRFTGVTPMAVNYRNPASLNFDSLNSFGAYRSPSSFYPLRRPLELSSLRRNRSSFFNTHRFDLGSNYTSFTPRYRSPLRGGLSQRFPLRSSWSKEF" }, "paralog": false, "identity": 0.972318339100346, "score": 768.0, "evalue": 8.54e-277, "description": "Internal stop codon at 549132, 549390. Nonsense mutation around 549132, 549390.", "psc": { "uniref90_id": "UniRef90_Q50360", "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "uniref50_id": "UniRef50_Q50360", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50360", "UniRef:UniRef50_Q50360", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q50360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50360" ], "product": "Cytadherence high molecular weight protein 3" } }, "id": "CNOCLNGIAL_991", "locus": "GCA000143945_00976" }, { "type": "cds", "sequence": "contig_1", "start": 550825, "stop": 551604, "strand": "-", "frame": 3, "gene": null, "product": "P30 adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75330" ], "nt": "ATGCTAGTGCTGTTCAGCGCTTTAATAGTGCTTGCAACCTTAATTTTGGTACAGCACAACAATACCGAACTGACAGAAGTTAAGAGTGAATTGAGTCCCCTTAACGTTGTTTTACACGCAGAAGAGGATACAGTACAAATTCAGGGCAAGCCGATTACTGAGCAAGCATGGTTTATTCCTACAGTTGCTGGTTGCTTTGGTTTTAGTGCCCTAGCCATCATCTTGGGCCTTGCTATAGGACTGCCAATTGTGAAGCGCAAGGAAAAACGCTTATTGGAGGAAAAGGAACGCCAAGAACAGTTAGCGGAACAGCTACAACGCATTTCTGCCCAACAAGAAGAGCAACAAGCGTTAGAACAACAAGCAGCTGCTGAAGCCCATGCTGAAGCGGAAGTTGAACCAGCACCACAACCAGTACCAGTACCACCTCAACCCCAAGTCCAAATTAACTTCGGTCCCCGTACTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCAGGTATGCCGCCACACCCCGGTATGGCTCCAAGACTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCAGGTATGCCGCCACACCCCGGTATGGCTCCAAGACCTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCAGGTATGCCGCCACACCCCGGTATGGCTCCAAGACCTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCCGGAATGCAACCACCACGTCCTGGCATGCCACCCCAACCCGGTTTTCCACCAAAACGCTAATTAG", "aa": "MLVLFSALIVLATLILVQHNNTELTEVKSELSPLNVVLHAEEDTVQIQGKPITEQAWFIPTVAGCFGFSALAIILGLAIGLPIVKRKEKRLLEEKERQEQLAEQLQRISAQQEEQQALEQQAAAEAHAEAEVEPAPQPVPVPPQPQVQINFGPRTGFPPQPGMAPRPGMPPHPGMAPRLVSHLNPVWRLVQVCRHTPVWLQDLVSHLNPVWRLVQVCRHTPVWLQDLVSHLNPVWRLVPECNHHVLACHPNPVFHQNAN", "aa_hexdigest": "3133ba933e9847967aa160a10f5f441f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9XDC1", "query_cov": 1.0926640926640927, "subject_cov": 1.029090909090909, "identity": 0.735, "score": 258.0, "evalue": 4.49e-83, "valid": false, "product": "Mutant adhesin-related protein P30", "uniref50_id": "UniRef50_P75330" }, "pscc": { "uniref50_id": "UniRef50_P75330", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75330" ], "product": "P30 adhesin" }, "genes": [], "id": "CNOCLNGIAL_992", "locus": "GCA000143945_00977" }, { "type": "cds", "sequence": "contig_1", "start": 551673, "stop": 552254, "strand": "-", "frame": 1, "gene": null, "product": "MG319/MPN454 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014325547.1", "SO:0001217", "UniParc:UPI0001DD63F5", "UniRef:UniRef100_A0AAX0SQD6", "UniRef:UniRef50_P47561", "UniRef:UniRef90_P75329" ], "nt": "ATGGGTTTCTTCAAATTGCTCTTTAAGCTAGCCTTCTTAGCGGTACTGACGGTAGCAATTGCTTACCTCTTTTTAGCAATCTTTTACTTTGGTACTGTTAGCCCATCCTTTAAACTAGCACAACCAATGGATGTGTTTAACCGTTTTTTCTCTAAGGAAGCGCTTGACACGGTTACTACTAGTGGTGTAGGCGCTACGGTAACTGCACCTAAAGCAGCTGCAGCATCACAGTCCCACGCCTTGATACTAGCAGCAGAACAAGTAGCTAATAATGCTAACAAGCTAGATCCCCGCTTTCCGATTGATGGTAGTTTGTTTAGCCAAATCCCAGGGTATGTCGACTTTTTAAAGAAACCCAATATCCCCGCCTTTTTAGAAAAGGTTGGTCCCTACCTTACTAAGTACATTATTCCGTTGGGCATGGCCTTAGTTTCAGGGCTTATAGGTGCCTTAATTATTAACTTTATCCTGTCCAAGATTACCCGTGCCATCATCAAAAAGCGCCGTAAAGCAAAACGGGCACAACAAAGTCGTGATGATGATTACTACTATGATGAAGCACCCCGTCGCCGCCGTCGTTAG", "aa": "MGFFKLLFKLAFLAVLTVAIAYLFLAIFYFGTVSPSFKLAQPMDVFNRFFSKEALDTVTTSGVGATVTAPKAAAASQSHALILAAEQVANNANKLDPRFPIDGSLFSQIPGYVDFLKKPNIPAFLEKVGPYLTKYIIPLGMALVSGLIGALIINFILSKITRAIIKKRRKAKRAQQSRDDDYYYDEAPRRRRR", "aa_hexdigest": "aed0d4a4d258b1ca7b40e9a5501c3336", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63F5", "ncbi_nrp_id": "WP_014325547.1", "uniref100_id": "UniRef100_A0AAX0SQD6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63F5", "RefSeq:WP_014325547.1", "UniRef:UniRef100_A0AAX0SQD6" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQD6", "uniref90_id": "UniRef90_P75329" }, "psc": { "uniref90_id": "UniRef90_P75329", "product": "MG319/MPN454 family protein", "uniref50_id": "UniRef50_P47561", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47561", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47561" ], "product": "Uncharacterized protein MG319" }, "genes": [], "id": "CNOCLNGIAL_993", "locus": "GCA000143945_00978" }, { "type": "cds", "sequence": "contig_1", "start": 552343, "stop": 552645, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAACTTTACGGTGTTCCGCGGTGGTCGCACTAATATCTTGGATTTACTGTTCCCCGGACTTTCGTTAATAGTTGGTGGGATCTTTTCCTTGTTATCGGTTTACTTTGTTTGTCTACAAATTCGCAACGAACAGGACTTGGTGAAACTGTATGAAAGTCAAGACCCAGCTTTAGCCAAAGCACAAATGTGGCGTAGTGCGCTCTTTTTTGGCGCTTGGTTGGTAAGTAGCTTCATGAACTTGGTCTACTTTATCCTGCGCATTATTTTGTTAACGCGTAATTTAAGCAGAGCTTTTTAA", "aa": "MLNFTVFRGGRTNILDLLFPGLSLIVGGIFSLLSVYFVCLQIRNEQDLVKLYESQDPALAKAQMWRSALFFGAWLVSSFMNLVYFILRIILLTRNLSRAF", "aa_hexdigest": "9c4b94a219939d2077e8cef7ce3052bb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11483.538399999992, "isoelectric_point": 9.851107597351074 }, "id": "CNOCLNGIAL_994", "locus": "GCA000143945_00979" }, { "type": "cds", "sequence": "contig_1", "start": 552685, "stop": 553206, "strand": "-", "frame": 3, "gene": null, "product": "putative MG320-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47562", "UniRef:UniRef90_P75328" ], "nt": "ATGATCAATCAAGCTAATAATCAGGTCAATTTTTCCGAGCAACAGTTTGTCCATCACAAGCGGTTCTCGGTCATTCGCTTGACCTTTTCTGTTGCCGCGATTGGCATTCTGTTTATCTTCTTAATTGGTTTTGGGGTACAACAGCTGTTAACCAACACTACTAGCCTTGGCACACTGGCTAGTGATATAAGAACGTTGGGTACGATTGCTTTTGTTGCTAGTTTGGTTTCCTTAATCCTTTACTTTGTCACGGCTTTTAAACTAAGGAACCGTAACACGACCTTAGCGTGGTTCTGGGGCTTAATTATTGCCGATGTGATTAGTTATGGGATTACATTAGGAGTATTGTTAACATTAGCCACCACACAATTACGTGAATTGATTGACTTTAAGTTTAGTGACATTGTCTTTGCCTTTTTAGGGGCAGCATTAGTGTATGGGACGGTATGGGGCTTGAGTGCATTACCGAGCCAGCAACGCCGTTACCAACAAACCCAAACGCTGTTTCGTATCTTTATTTGA", "aa": "MINQANNQVNFSEQQFVHHKRFSVIRLTFSVAAIGILFIFLIGFGVQQLLTNTTSLGTLASDIRTLGTIAFVASLVSLILYFVTAFKLRNRNTTLAWFWGLIIADVISYGITLGVLLTLATTQLRELIDFKFSDIVFAFLGAALVYGTVWGLSALPSQQRRYQQTQTLFRIFI", "aa_hexdigest": "a3768fd76e5e46b3b01c7b669d5381d5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 552382, "stop": 553206, "observations": { "mutation": [ 552687 ], "stop": [ 552687 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75328", "query_cov": 1.0, "subject_cov": 0.6027874564459931, "identity": 1.0, "score": 324.0, "evalue": 2.55e-110, "gene_start": 1, "gene_end": 173, "reference_start": 1, "reference_end": 173, "reference_sequence": "MINQANNQVNFSEQQFVHHKRFSVIRLTFSVAAIGILFIFLIGFGVQQLLTNTTSLGTLASDIRTLGTIAFVASLVSLILYFVTAFKLRNRNTTLAWFWGLIIADVISYGITLGVLLTLATTQLRELIDFKFSDIVFAFLGAALVYGTVWGLSALPSQQRRYQQTQTLFRIFIWAFFISIIASLLTFVLNFTVFRGGRTNILDLLFPGLSLIVGGIFSLLSVYFVCLQIRNEQDLVKLYESQDPALAKAQMWRSALFFGAWLVSSFMNLVYFILRIILLTRNLSRAF" }, "paralog": false, "identity": 0.9963503649635036, "score": 526.0, "evalue": 3e-192, "description": "Internal stop codon at 552687. Nonsense mutation around 552687.", "psc": { "uniref90_id": "UniRef90_P75328", "product": "Uncharacterized protein MG320 homolog", "uniref50_id": "UniRef50_P47562", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75328", "UniRef:UniRef50_P47562", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47562" ], "product": "Uncharacterized protein MG320" } }, "id": "CNOCLNGIAL_995", "locus": "GCA000143945_00980" }, { "type": "tRNA", "sequence": "contig_1", "start": 553276, "stop": 553366, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(gga)", "amino_acid": "Ser", "anti_codon": "gga", "score": 69.6, "nt": "GGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAACACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 553276, 553278 ], "id": "CNOCLNGIAL_13", "locus": "GCA000143945_00981" }, { "type": "tRNA", "sequence": "contig_1", "start": 553368, "stop": 553456, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 75.0, "nt": "GGAGACTTACCCAAGTGGTTTAAGGGGGCGCTCTCGAAAAGCGTTAGGTGTTTTTGCATGCGTGGGTTCGAATCCCACAGTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 553402, 553404 ], "id": "CNOCLNGIAL_14", "locus": "GCA000143945_00982" }, { "type": "cds", "sequence": "contig_1", "start": 553724, "stop": 554317, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACGCAGCCACCCAATTGAACGATCAAGGTGGCGGTGATGTTATTACCTACTCCTCGGGCGCTCACATTGTACGTACGTTGTTGTTAGCTGCGAGTCAAAAAGATGTACATAGTAAATTCACACAAAAAATAGATCAACAAAATACAGCTTCAACCACATCTGATGTGACTGTTAAGAAAGCAGATTCATCTCAAGATAGCAGCAAGAGTAATACTGAAGAAGAAAAGTGGGATGACGTAACTTCCGCTGACCTCTTCAAAGATGACCCTTACGTACTAAAGAACTTTGGTGATGCTAAAGCACAAGCTGCACAAAGAAGCACCGGTTCTACAACTAGTGGTAACGGCACTCAAGCATCTTTAGAGTTCACCAAAAAGGCTTTAAGCTTGCTCAAATTCTTAGTGGATAATGGCATTCTAGACAAAGAAAAAGTAAAGGAAGCTATCAAAGATCCAAATAAGTACCTTGGAAAGCGTGACAAGATAGAGAACGGTACAAATAAACCAAGTAAAAACGAAGATTTCATTGGTTACGAGTTGAAAGATATCTATAAGAACGCCGCCCAGCTCAACCGCTTTAACAGTATTTGA", "aa": "MDAATQLNDQGGGDVITYSSGAHIVRTLLLAASQKDVHSKFTQKIDQQNTASTTSDVTVKKADSSQDSSKSNTEEEKWDDVTSADLFKDDPYVLKNFGDAKAQAAQRSTGSTTSGNGTQASLEFTKKALSLLKFLVDNGILDKEKVKEAIKDPNKYLGKRDKIENGTNKPSKNEDFIGYELKDIYKNAAQLNRFNSI", "aa_hexdigest": "adf1f0a43c5c833a608a8b021a19c4b0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21577.56740000001, "isoelectric_point": 6.1571065902709945 }, "id": "CNOCLNGIAL_996", "locus": "GCA000143945_00983" }, { "type": "cds", "sequence": "contig_1", "start": 554348, "stop": 554983, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAACTCCGGTGACCTGAACTATTCGATCGTACCTTTTGGCATCTTTAAGGAAAAGGGTAAAAATGGTGCAAGTGTTCAACAAAAAGCTGTCTCAACTACCGAAGGTTCAGATCTGATGAACGATTACTACTATAAGATCGAGAAGGAACAGCGCCTGGGCTTGATTCCACAGCGTGAAGGTAACTACGAAAAGAATAAAAACGTTTTGGAGAGTGCGACTGTTAAGATTAATTATTATAGCAGTAAAGCAACAAGTGGTCAGGCTGGAGCAGCTGCCTCTGCAGCGTTTGCTAAGAACAACAACACATCAGACAACACACAACAAAACCAAACCTCTTCAGTAGAAGCTAAGAGTGTAAACGTAACAAAGCACAGCTTTGTTCAAGTTTTAAAGAAGGTTGGTTTCTCTGGTAGCAATCCCCTCCACTTCAACATGAAGTTAGGGAACTCTTCGCTGTCCGCTAACGGGGTGGATTACTACAACGCCGTGAAGCAAGCCCTCACTGAATTGGGTACTGCTGACAACGGAGAAAAACTAATAGTACCAGAAATTATCTTAGGTGATGCCCAAGGACCAACTCGAAATGAGTGGTACATTGGGTTGTCCTCGGTGTTAGGTTTTAGTTCCTGA", "aa": "MPNSGDLNYSIVPFGIFKEKGKNGASVQQKAVSTTEGSDLMNDYYYKIEKEQRLGLIPQREGNYEKNKNVLESATVKINYYSSKATSGQAGAAASAAFAKNNNTSDNTQQNQTSSVEAKSVNVTKHSFVQVLKKVGFSGSNPLHFNMKLGNSSLSANGVDYYNAVKQALTELGTADNGEKLIVPEIILGDAQGPTRNEWYIGLSSVLGFSS", "aa_hexdigest": "df1254b64e210ba881269a76d4bde850", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22749.990199999993, "isoelectric_point": 8.58217639923096 }, "id": "CNOCLNGIAL_997", "locus": "GCA000143945_00984" }, { "type": "cds", "sequence": "contig_1", "start": 555005, "stop": 555460, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGCCTTACTATGTCGAGAGTTTTAACCAGAGCCAGATTGTCTTTAAGCGTAACAGTGAGTATGATACTCATATAACCCCCAACTTACCAAAAACGCGGGAAGAAAACGAAAAACCCATCCCAACGATGATTAACTACTTCCAACCAGGCGCTACCCCTGAAGTGTTTTACTCCAACTATATCGCTGGAGGATTATCTTCTGCGGAGGTGCCATACTCCCAACAAGAGGATGCTAGGTCACGATTTGCGGGTACGGGGGACTTACGCTGGGTTAAAGTGCAAAAGACCGCGCAAAGTGCCCAGATTACCTATTCATCACGACCTTACGTAGTGGAAGGTGAAACCGTTAAAACTAACTCCAACATTACCGAAACCGAGGCTAAATTCTTGTACAACAGTGAGTCAGAAGAAGCGTTAACGATTCGCGCTGGCATTAACGGTTTAATTAACTGA", "aa": "MGPYYVESFNQSQIVFKRNSEYDTHITPNLPKTREENEKPIPTMINYFQPGATPEVFYSNYIAGGLSSAEVPYSQQEDARSRFAGTGDLRWVKVQKTAQSAQITYSSRPYVVEGETVKTNSNITETEAKFLYNSESEEALTIRAGINGLIN", "aa_hexdigest": "751b86784319df3985cddd785068e8b1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17005.5827, "isoelectric_point": 5.081542396545408 }, "id": "CNOCLNGIAL_998", "locus": "GCA000143945_00985" }, { "type": "cds", "sequence": "contig_1", "start": 555476, "stop": 555853, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTGGATGTGAAAAAGACCGTGGGGATGACCAAGAAGAATGGTACCCAAGTTCAAATGAAGGTTGCTTCATCTGATGAAAAAGATGGTGAAGAAAAAACTGTTAAGATAACGGATGATGCTTACAATCCTGAGGATTATCAAAGCACTGATGACAGCAAATTCAACGTCTACCTCACCTCGCCCTTCCCCTTCTTGTTGAGTATGATGTCTAAGGAATTCTTCTTTCCTATTCCTCATACCCACCCGAAGGTAAAAGCAATTAAGGTAGGTAAGGACTCACCATTGGTCTACAACGAAAAGAATGGGTCGAAAATCTTAGACCAAACCAAAACTAACTTTGATGGTATCTATGGTGGTGGGGTTAACGCATGA", "aa": "MGLDVKKTVGMTKKNGTQVQMKVASSDEKDGEEKTVKITDDAYNPEDYQSTDDSKFNVYLTSPFPFLLSMMSKEFFFPIPHTHPKVKAIKVGKDSPLVYNEKNGSKILDQTKTNFDGIYGGGVNA", "aa_hexdigest": "42b0ac68c334af1a8071d8df97aa7caf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13890.575299999986, "isoelectric_point": 6.726576423645019 }, "id": "CNOCLNGIAL_999", "locus": "GCA000143945_00986" }, { "type": "cds", "sequence": "contig_1", "start": 556142, "stop": 556549, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTCAGCGAAAATACTGGGGTTTGTTATCAACCTTAGGGGTAAGTTCAGCAGTAGCACTCAGTGCCTGCGCTGCCCAAGCCCGCGATGTGTATGTCACCTCATCCGCATCCGACTTGTTAAAGAACAATTCGGTACCGATGAGTATGTTCAACGTGTCCCCGACCTCTTCCTTTTTTGGCAGCAAGTATGCAGGCCTAACCACTTACATTGCCACTGGTTCCAACAAAGATGATGGTGTCAATGTAACCACTCAAACCCAAGAGAAATTGGTGTTGGAATTGGCTACCTCAGTGAAGGGGTATAAGAAGAAGGATAAAGCTACTTCAAGTCAAAAAACTTCAACTAATTCTTCTTGCACTACCACTTCTAGTGGTACTAGCACTAGTGGTGAAGATGATTGA", "aa": "MKFQRKYWGLLSTLGVSSAVALSACAAQARDVYVTSSASDLLKNNSVPMSMFNVSPTSSFFGSKYAGLTTYIATGSNKDDGVNVTTQTQEKLVLELATSVKGYKKKDKATSSQKTSTNSSCTTTSSGTSTSGEDD", "aa_hexdigest": "2377da1e52e23b097a7147ead82f8f3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14212.632599999977, "isoelectric_point": 9.098052406311037 }, "id": "CNOCLNGIAL_1000", "locus": "GCA000143945_00987" }, { "type": "tRNA", "sequence": "contig_1", "start": 556773, "stop": 556857, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 64.4, "nt": "GCACTCGTGGCGGAATGGTAGACGCGCTAGACTTAGGATCTAGTTTCATTGCGGAGTGGGGGTTCAAGTCCCTTCGAGTGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 556837, 556839 ], "id": "CNOCLNGIAL_25", "locus": "GCA000143945_00988" }, { "type": "tRNA", "sequence": "contig_1", "start": 556862, "stop": 556937, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 85.2, "nt": "GCATCTTTAGCTCAGTTGGTAGAGCAAATGACTCTTAATCATTGGGTCGGGGGTTCGAGCCCCTCAAGATGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 556931, 556933 ], "id": "CNOCLNGIAL_24", "locus": "GCA000143945_00989" }, { "type": "tRNA", "sequence": "contig_1", "start": 556950, "stop": 557025, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 89.8, "nt": "GCCGACTTAGCTCAGTTGGTAGAGCAATTGACTTGTAATCAGTAGGTCGTAGGTTCGAATCCTATAGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 556990, 556992 ], "id": "CNOCLNGIAL_23", "locus": "GCA000143945_00990" }, { "type": "tRNA", "sequence": "contig_1", "start": 557027, "stop": 557102, "strand": "-", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 81.7, "nt": "GGATTGTTAGCTCAGCTGGGAGAGCATATGCCTTACAAGCATAGGGTCGGGGGTTCGATCCCCTCACAATCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 557067, 557069 ], "id": "CNOCLNGIAL_22", "locus": "GCA000143945_00991" }, { "type": "tRNA", "sequence": "contig_1", "start": 557110, "stop": 557184, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 81.5, "nt": "GCCGAATTAGCTCAAGGGTAGAGCGATGCACTCGTAATGCATAGGCTACAGGTTCAATTCCTGTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 557150, 557152 ], "id": "CNOCLNGIAL_21", "locus": "GCA000143945_00992" }, { "type": "tRNA", "sequence": "contig_1", "start": 557186, "stop": 557262, "strand": "-", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 58.9, "nt": "GGCGCGTTGGTGAAGAGACTTAACACACACGCCTTTCACGCGTGCATTCACGGGTTTGAATCCCGTACGCGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 557226, 557228 ], "id": "CNOCLNGIAL_20", "locus": "GCA000143945_00993" }, { "type": "tRNA", "sequence": "contig_1", "start": 557271, "stop": 557345, "strand": "-", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 84.0, "nt": "GGCCACATAGCTCAGCGGTAGAGCAACCGGCTGTTAACCGGTTGGTCACAGGTTCGATCCCTGTTGTGGCCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 557311, 557313 ], "id": "CNOCLNGIAL_19", "locus": "GCA000143945_00994" }, { "type": "cds", "sequence": "contig_1", "start": 557373, "stop": 557852, "strand": "-", "frame": 1, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D97117", "UniRef:UniRef90_UPI0027D97117" ], "nt": "ATGGTGATTGCGATGTTTATCGGGGGCAGTCCGTCTTCTACCGCCGGAGGGATTCGCACTACTACTTTAGCGGTCATCTTTTTGACGTTAAAAGCGAAGTTCCGCGGTCAAAAAGAGGTCAAGGTCTTTAAGCGTAGTATTGACGGGCAAACGGTGATTAATGCCTTCTTGGTAGCCGTCTTTGGCTTAGTGTCCGTGGTTCTTATTGCCATTCTGTTGCCGTTAAGTATGCAACAACCGCTTAGCTTTGTCGACAGCCTGTTTGAAACCACTTCTGCTTTTGGAACTGTCGGACTGTCTAGTGGGGCCACGAAGATCATGGCACTAGAGCCAACACGGAACCTGTTTAACTACTTAACGTTAGGTTTACTAATGATTATGGGGCAGGTCGGGGTGAGCAGTTCGGTACTCACTTTTGTTAAAAAACACCCTCAAGGAAACAGCTTTTCTTATCCGCGGGAAGATGTTAAAGTGGGCTAG", "aa": "MVIAMFIGGSPSSTAGGIRTTTLAVIFLTLKAKFRGQKEVKVFKRSIDGQTVINAFLVAVFGLVSVVLIAILLPLSMQQPLSFVDSLFETTSAFGTVGLSSGATKIMALEPTRNLFNYLTLGLLMIMGQVGVSSSVLTFVKKHPQGNSFSYPREDVKVG", "aa_hexdigest": "8d738b66a126f157973e098d96f98f66", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 557373, "stop": 558011, "observations": { "mutation": [ 557885 ], "stop": [ 557885 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0027D97117", "query_cov": 1.0, "subject_cov": 0.7464788732394366, "identity": 1.0, "score": 295.0, "evalue": 3.01e-100, "gene_start": 1, "gene_end": 159, "reference_start": 55, "reference_end": 213, "reference_sequence": "VLKNKSNQELQSLIFGPIPAASRVMQLWFGVISSRSAGFSVFPWSAESDIIKGIMVIAMFIGGSPSSTAGGIRTTTLAVIFLTLKAKFRGQKEVKVFKRSIDGQTVINAFLVAVFGLVSVVLIAILLPLSMQQPLSFVDSLFETTSAFGTVGLSSGATKIMALEPTRNLFNYLTLGLLMIMGQVGVSSSVLTFVKKHPQGNSFSYPREDVKVG" }, "paralog": false, "identity": 0.9952830188679245, "score": 400.0, "evalue": 6.97e-144, "description": "Internal stop codon at 557885. Nonsense mutation around 557885.", "psc": { "uniref90_id": "UniRef90_UPI0027D97117", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI0027D97117", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0027D97117", "UniRef:UniRef50_UPI0027D97117" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0027D97117", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D97117" ], "product": "potassium transporter TrkG" } }, "id": "CNOCLNGIAL_1001", "locus": "GCA000143945_00995" }, { "type": "cds", "sequence": "contig_1", "start": 557883, "stop": 558632, "strand": "-", "frame": 1, "gene": "trkG", "product": "putative cation transporter MG322-like protein", "db_xrefs": [ "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001", "SO:0001217", "UniRef:UniRef50_P75323", "UniRef:UniRef90_P75323" ], "nt": "ATGTTGCAATCAGAACGGGGTGGTTCCAAGTTAGGGACCACTAGTGAAATGATTGTGGTGTCGGTGTTATTCCTCTTTATGGTGGAGTTACTGTATGGCTTTCTGTACACCATTTTGTTTTATTTTATCCCCGCGTTTGAGTCTGCTAGTGTTTTTCAAAGTAGTGGTAAGGTGTCCAACCAATTGAAGGCCCTCATAGTCGATTCCACCAAAAGATTACCCGTAGTTCATAACCTTAACTTAGCCTTTCAGTATGGTTTTTTCCACTCCTTGTCAGCGGTTAATAATGCTGGCATTGACCTGTTGGGCGCTAATTCGTTTGCCCCGTATCGCACTAACTGGGGCATCGTGATCCAGTGGTTAGCGATTAGCCAAATTATCTTTGGTGGCATTGGTTATCCGGTGTTATTTGATGCTTATGAAGCGATTAAGAAACGGCGATTGTATGGTAAGTATTACAAACACCAGTTTAGTTTGTTTACGAAGTTAGCGGTACTCACTAACCTGATTGTCACGGCTTGGTGTTTTTTAATGCTGTTAATGGTGGAGTTTATTGTTATAACTAGTTTGACCAATACGATTGCCCATCTCAATGTCGAGAAGGCCTACTTAGTGGAGGGACTGAAAAATAAATCCAATCAAGAACTCCAAAGCTTAATCTTTGGTCCCATACCAGCGGCTAGTCGGGTGATGCAACTGTGGTTTGGGGTGATTTCGTCCCGGTCCGCTGGCTTTAGTGTGTTTCCCTGA", "aa": "MLQSERGGSKLGTTSEMIVVSVLFLFMVELLYGFLYTILFYFIPAFESASVFQSSGKVSNQLKALIVDSTKRLPVVHNLNLAFQYGFFHSLSAVNNAGIDLLGANSFAPYRTNWGIVIQWLAISQIIFGGIGYPVLFDAYEAIKKRRLYGKYYKHQFSLFTKLAVLTNLIVTAWCFLMLLMVEFIVITSLTNTIAHLNVEKAYLVEGLKNKSNQELQSLIFGPIPAASRVMQLWFGVISSRSAGFSVFP", "aa_hexdigest": "2e8f4cfaf0c0bd006df29af5d3a18073", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "both", "pseudogene": { "start": 557580, "stop": 558932, "observations": { "mutation": [ 557885, 558686 ], "stop": [ 557885, 558686 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75323", "query_cov": 1.0, "subject_cov": 0.43915343915343913, "identity": 1.0, "score": 477.0, "evalue": 4.5e-165, "gene_start": 1, "gene_end": 249, "reference_start": 147, "reference_end": 395, "reference_sequence": "MTAAHKQKAKLLAWLKLILWGDSISQRIFHFYIYCILLGAVLLFLPFALKTDYQKVISYEVDLQGHTISKQTASYGFLDALFLAVSAFSDTGLSTTVVSETYSVFGQTVLAILLQLGGIGFVVIAFLVWRLFKLHKKGKYSFYEKLMLQSERGGSKLGTTSEMIVVSVLFLFMVELLYGFLYTILFYFIPAFESASVFQSSGKVSNQLKALIVDSTKRLPVVHNLNLAFQYGFFHSLSAVNNAGIDLLGANSFAPYRTNWGIVIQWLAISQIIFGGIGYPVLFDAYEAIKKRRLYGKYYKHQFSLFTKLAVLTNLIVTAWCFLMLLMVEFIVITSLTNTIAHLNVEKAYLVEGLKNKSNQELQSLIFGPIPAASRVMQLWFGVISSRSAGFSVFPWSAESDIIKGIMVIAMFIGGSPSSTAGGIRTTTLAVIFLTLKAKFRGQKEVKVFKRSIDGQTVINAFLVXXXVFGLVSVVLIAILLPLSMQQPLSFVDSLFETTSAFGTVGLSSGATKIMALEPTRNLFNYLTLGLLMIMGQVGVSSSVLTFVKKHPQGNSFSYPREDVKVG" }, "paralog": false, "identity": 0.9889380530973452, "score": 851.0, "evalue": 8.27e-315, "description": "Internal stop codon at 557885, 558686. Nonsense mutation around 557885, 558686.", "psc": { "uniref90_id": "UniRef90_P75323", "gene": "TrkG", "product": "Uncharacterized cation transporter MG322 homolog", "uniref50_id": "UniRef50_P75323", "cog_id": "COG0168", "cog_category": "P", "go_ids": [ "GO:0005886", "GO:0008324", "GO:0030001" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75323", "UniRef:UniRef50_P75323", "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001" ] }, "pscc": { "uniref50_id": "UniRef50_P75323", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75323" ], "product": "Uncharacterized cation transporter MG322 homolog" } }, "id": "CNOCLNGIAL_1002", "locus": "GCA000143945_00996" }, { "type": "cds", "sequence": "contig_1", "start": 558684, "stop": 559070, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGCTGCTCATAAACAGAAAGCTAAACTACTCGCTTGGTTAAAACTAATCCTGTGGGGTGACAGTATTTCCCAGCGCATTTTCCACTTTTACATCTATTGCATCCTGTTGGGGGCTGTACTGCTTTTCCTCCCGTTTGCGCTCAAAACCGACTACCAAAAGGTTATTAGCTATGAGGTGGATTTACAGGGTCATACCATCAGTAAACAAACGGCTTCCTATGGCTTTTTGGATGCTTTGTTTTTAGCGGTCAGTGCCTTTAGTGATACGGGGCTCTCTACTACGGTAGTGAGTGAAACCTACAGTGTGTTTGGGCAAACGGTGTTGGCCATTTTACTGCAGTTAGGGGGCATTGGTTTTGTGGTGATTGCTTTCCTTGTTTGA", "aa": "MTAAHKQKAKLLAWLKLILWGDSISQRIFHFYIYCILLGAVLLFLPFALKTDYQKVISYEVDLQGHTISKQTASYGFLDALFLAVSAFSDTGLSTTVVSETYSVFGQTVLAILLQLGGIGFVVIAFLV", "aa_hexdigest": "ebe8dac325ed4fa585280446d063174f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14113.476699999981, "isoelectric_point": 7.861161994934083 }, "id": "CNOCLNGIAL_1003", "locus": "GCA000143945_00997" }, { "type": "cds", "sequence": "contig_1", "start": 559105, "stop": 559800, "strand": "+", "frame": 1, "gene": "trkA", "product": "putative MG323-like protein", "db_xrefs": [ "COG:COG0569", "COG:PT", "GO:0006813", "GO:0008324", "RefSeq:WP_010874817.1", "SO:0001217", "UniParc:UPI000013961A", "UniRef:UniRef100_P75322", "UniRef:UniRef50_P75322", "UniRef:UniRef90_P75322" ], "nt": "ATGAAACGTGCCGATTTTTGCATTATTGGCCTTGGTAGGTTTGGGATGCAAGTAGCACATTCTTTAAAAGAGAACAACTTTACCCTGGTTTTGATCGATAATGATCAACACAAAACCAATACTGCTGCCCAGGAATTTGACCATGTAGTGTGTTGTGATGGGAGTAACTTAACGGCGTTAGCTGAGCTCCAACTAGAGGAGTTTTCGGCAGTCATAGTTGGGGTAACTAACATAGAAGCGAGCATTATGATTTGTGCTAACCTTCGGGAATTGGGTCAAAAAAACATTATTGCCCGTGCTAAAAACGAAGTGCATAACCGGGTGTTACGTACTATGGGTATTAGGGAAGCTTTGATCCCGGAAAAGATTGTCGGCAAGAACCTTGTCATTCGTTTAATTCACGGCATGGAAACCGAAATTATTAACCTTGGCAATGACATCCTCTTTGTTCGCGCTCCGGTGAACAACAAGAAGCTCTTTAATCGTACGTTGGGGGAAATTAACATCCGTGAACACACGAATGCTAACCTCATCTCAATTATGCGGAATGGCAAAGATGTCGTCTTTCCTTTGGGTCCGAATACCCAGATTCAACCAGGGGATGTAATTACAGCCGTGTGTCAATTAAGTGGCGTGAACCAGTATTTGCGCTACATTAATCCTAACGACAAGAATAAGTACAAAGCGAGTGAATAA", "aa": "MKRADFCIIGLGRFGMQVAHSLKENNFTLVLIDNDQHKTNTAAQEFDHVVCCDGSNLTALAELQLEEFSAVIVGVTNIEASIMICANLRELGQKNIIARAKNEVHNRVLRTMGIREALIPEKIVGKNLVIRLIHGMETEIINLGNDILFVRAPVNNKKLFNRTLGEINIREHTNANLISIMRNGKDVVFPLGPNTQIQPGDVITAVCQLSGVNQYLRYINPNDKNKYKASE", "aa_hexdigest": "ed910f8d08c6738e9a02d4fb3d1effa7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013961A", "ncbi_nrp_id": "WP_010874817.1", "uniref100_id": "UniRef100_P75322", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013961A", "RefSeq:WP_010874817.1", "UniRef:UniRef100_P75322" ] }, "ips": { "uniref100_id": "UniRef100_P75322", "uniref90_id": "UniRef90_P75322" }, "psc": { "uniref90_id": "UniRef90_P75322", "gene": "TrkA", "product": "Uncharacterized protein MG323 homolog", "uniref50_id": "UniRef50_P75322", "cog_id": "COG0569", "cog_category": "PT", "go_ids": [ "GO:0008324", "GO:0006813" ] }, "pscc": { "uniref50_id": "UniRef50_P75322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75322" ], "product": "Uncharacterized protein MG323 homolog" }, "genes": [ "trkA" ], "id": "CNOCLNGIAL_1004", "locus": "GCA000143945_00998" }, { "type": "cds", "sequence": "contig_1", "start": 559862, "stop": 560296, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_462", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321", "UniRef:UniRef50_UPI001375E5B8", "UniRef:UniRef90_P75321" ], "nt": "GTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAACAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGA", "aa": "MVYYEQLQRGLALPNQGSSSGSDSTNQTGAMFGLKVKDATVDSSKQSTESLKGEESSSSSTTSSTSTTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSESGQSGTPTP", "aa_hexdigest": "41565d62a1343546e1f95d063ffe9e18", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001375E5B8", "query_cov": 0.9097222222222222, "subject_cov": 0.8618421052631579, "identity": 0.6559999999999999, "score": 142.0, "evalue": 1.13e-40, "valid": false, "uniref50_id": "UniRef50_UPI001375E5B8" }, "pscc": { "uniref50_id": "UniRef50_UPI001375E5B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001375E5B8" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 559862, "stop": 560599, "observations": { "mutation": [ 560294, 560342, 560456, 560471, 560495 ], "stop": [ 560294, 560342, 560456, 560471, 560495 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75321", "query_cov": 1.0, "subject_cov": 0.48, "identity": 0.993, "score": 226.0, "evalue": 9.11e-72, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MVYYEQLQRGLALPNQGSSSGSDSTNQTGAMFGLKVKDATVDSSNQSTESLKGEESSSSSTTSSTSTTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSESGQSGTPTPWTPWLATEQIHKDLPKWSASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDQGQAVADNTQTGFDTDDTDNKKQGFRKQGEQSPAPIALPFEAYFANIGNLTWFGQALLVFGICLS" }, "paralog": false, "identity": 0.9714285714285714, "score": 434.0, "evalue": 2.6e-156, "description": "Internal stop codon at 560294, 560342, 560456, 560471, 560495. Nonsense mutation around 560294, 560342, 560456, 560471, 560495.", "psc": { "uniref90_id": "UniRef90_P75321", "product": "Uncharacterized protein MPN_462", "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75321", "UniRef:UniRef50_P75321" ] }, "pscc": { "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321" ], "product": "Uncharacterized protein MPN_462" } }, "id": "CNOCLNGIAL_1005", "locus": "GCA000143945_00999" }, { "type": "cds", "sequence": "contig_1", "start": 560276, "stop": 560731, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCGAACCAGGTGAGGTTGCCAATGTTGGCGAAGTACGCTTCAAACGGGAGGGCGATGGGGGCAGGGGATTGTTCGCCTTGTTTGCGAAAGCCCTGCTTTTTATTATCGGTGTCATCTGTATCAAACCCGGTTTGGGTATTATCCGCGACCGCTTGGCCCTGGTCAAACCACTCTGGGTGGCGGCGCGGGTTGAAGAACCCGGTGGTTTGGAGCAGGACATCGCGCGCCTTTCAGTCCCACAACCCGTGGTGGTTTCACTTGGGCGTTCTTCAACTGGGCGGATAGTTCGCGGTCATCACCTTGGGATCCAAGTGATCAACGCGATCAATGGCGGTACGATTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGATCATTTGGGGAGGTCCTTGTGAATTTGCTCGGTCGCAAGCCACGGCGTTCAGGGGGTGGGGGTGCCTGA", "aa": "MPEPGEVANVGEVRFKREGDGGRGLFALFAKALLFIIGVICIKPGLGIIRDRLALVKPLWVAARVEEPGGLEQDIARLSVPQPVVVSLGRSSTGRIVRGHHLGIQVINAINGGTIARIRRIVQNQDRGGSFGEVLVNLLGRKPRRSGGGGA", "aa_hexdigest": "a30dbf08a756d3c04d6bcdca05587bd0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16026.549699999983, "isoelectric_point": 11.366952705383298 }, "id": "CNOCLNGIAL_1006", "locus": "GCA000143945_01000" }, { "type": "cds", "sequence": "contig_1", "start": 560907, "stop": 561482, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGTCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAACTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGTCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCAGAAGTTAAATGAAGCTTGA", "aa": "MGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQVLQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYKTLQDLLVEQPVTPYTPNAGLVRVNGVAQDTVHFGSGQESSWNSQRSQKGLKNNPGPKAVTGFKLDKGRAYQKLNEA", "aa_hexdigest": "ca647ad375acb6498a92f0869d1fadab", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20772.74409999999, "isoelectric_point": 9.396218681335448 }, "id": "CNOCLNGIAL_1007", "locus": "GCA000143945_01001" }, { "type": "cds", "sequence": "contig_1", "start": 561486, "stop": 561812, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAACCCCTGGATTCGACGAAGGAGGGGAAGGAGAAAAACGAATCGGAGTGGAAGTCTTCCGGGGCGAGTGAGGCAAAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCTGTTACAGACAATCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCACAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MYEPLDSTKEGKEKNESEWKSSGASEAKNDAPLVGSTGSQMAAVTDNQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLET", "aa_hexdigest": "c75d6f54ce32396215e55f305233594a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11411.227499999995, "isoelectric_point": 4.809567832946779 }, "id": "CNOCLNGIAL_1008", "locus": "GCA000143945_01002" }, { "type": "cds", "sequence": "contig_1", "start": 562116, "stop": 562754, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGGGCAGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGTGCTCGCCTGGTCGACCACGGCCGGATTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGATTGAACGGACCGATTAATGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCCAAAATTGCTTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACATCCGATGATAATTCAAACACCAAAGTCAAGTGA", "aa": "MGFKDDSNSDSKNSNLKAQGLTQPAYLIAGLDVVADHLVFAAFKAGAVGYDMTTDSNASTKDQVLAWSTTAGLDSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSKNTEAVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLDEARLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVK", "aa_hexdigest": "6c57a213ded435cc3e8788e92409378d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.9292452830188679, "subject_cov": 0.8565217391304348, "identity": 0.9390000000000001, "score": 350.0, "evalue": 7.6e-121, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_1009", "locus": "GCA000143945_01003" }, { "type": "cds", "sequence": "contig_1", "start": 563507, "stop": 563869, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254", "UniRef:UniRef90_P75592" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAAACTCAAACAACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAGAGAACTTCAAGTAGAGCAAAAATTTCAGGGTGAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCGCTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAG", "aa": "MNTEYVTRKEFNEYKNSNNQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIRELQVEQKFQGEQIKAQGKTLKSILQALGGINKRLDKIDPPK", "aa_hexdigest": "4dbddefcbd4c1ec3dd8b4b788f5295c9", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 563339, "stop": 563869, "observations": { "mutation": [ 563420 ], "stop": [ 563420 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75592", "query_cov": 1.0583333333333333, "subject_cov": 0.6939890710382514, "identity": 0.8190000000000001, "score": 76.6, "evalue": 4.32e-15, "gene_start": 1, "gene_end": 120, "reference_start": 57, "reference_end": 183, "reference_sequence": "MKEKIPFYNEKEFHDMVKKTKKGTFSGWYIIDKDNKSVEFSGSFNRQFKLNKPVIPVNTEYVTRKEFNEYKVSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQVEQKAQGEQIKAQGKQIKAQGKTLKSILQALGGINKRLDKIDPPK" }, "paralog": false, "identity": 0.8469945355191257, "score": 184.0, "evalue": 6.48e-60, "description": "Internal stop codon at 563420. Nonsense mutation around 563420.", "psc": { "uniref90_id": "UniRef90_P75592", "product": "UPF0134 protein MPN_100", "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75592", "UniRef:UniRef50_P75254" ] }, "pscc": { "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254" ], "product": "UPF0134 protein MPN_524" } }, "id": "CNOCLNGIAL_1010", "locus": "GCA000143945_01004" }, { "type": "cds", "sequence": "contig_1", "start": 564335, "stop": 564424, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGATTGAACATTTCCAGATGACATACCAAAGTTTTAAGGATTTGTCAGTTGAGGATAAGTTAAGCTATACCTTTAATTGA", "aa": "MERKIEHFQMTYQSFKDLSVEDKLSYTFN", "aa_hexdigest": "2a16b359f26e6c4d1c4f87aadc43554c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3616.038300000001, "isoelectric_point": 5.53852787017822 }, "id": "CNOCLNGIAL_1011", "locus": "GCA000143945_01005" }, { "type": "cds", "sequence": "contig_1", "start": 564512, "stop": 564760, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75316", "UniRef:UniRef90_P75316" ], "nt": "ATGCGCAAAAAGAGATTGTTGTCAAGGATTTCCTTTAGTTCTCTATTTTTATTATGTGGAACATTGTTAAGTGCTTGCACTGGTATCCAAGCAGATCTTAGAAATCTTATTAAAGAAACCACTGGTAAAGACATTGATTTGTCTAAAGCAATTAAAACTAAAGAAGGCAAAAAGAACATAATAGCTTCTTTAAAAAAGTCTTACGAAGTTAATCCGAGAGATACAACAAAATTGTTGTTAGATGCTTGA", "aa": "MRKKRLLSRISFSSLFLLCGTLLSACTGIQADLRNLIKETTGKDIDLSKAIKTKEGKKNIIASLKKSYEVNPRDTTKLLLDA", "aa_hexdigest": "13dfe1101522dd7d52ba9752cb93c633", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 1.0, "score": 153.0, "evalue": 5.5e-47, "valid": true, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "CNOCLNGIAL_1012", "locus": "GCA000143945_01006" }, { "type": "cds", "sequence": "contig_1", "start": 565157, "stop": 565354, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0AAN4XEA6" ], "nt": "ATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGATACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGTTGACACTGAGACGACAAATAAAGAAAAGCCTTTAGGCACTTGA", "aa": "MKKVKALRNLFLEKASEKLEYPKIASMVAYFDPKEDDTCEFIMKIKTVNEVVDTETTNKEKPLGT", "aa_hexdigest": "9f1295d6f8b633aff52d35b521d941bb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XEA6", "query_cov": 1.0, "subject_cov": 0.8552631578947368, "identity": 0.9540000000000001, "score": 118.0, "evalue": 9.83e-34, "valid": true, "uniref50_id": "UniRef50_A0A0H3DJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7498.6667, "isoelectric_point": 6.245093345642091 }, "id": "CNOCLNGIAL_1013", "locus": "GCA000143945_01007" }, { "type": "cds", "sequence": "contig_1", "start": 565393, "stop": 565725, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCACCCACAGTCGTCGTCTGACTGTCACCAAGCGTGCCCTGGACATTCGCTTTGAGACCAACGCCGACATTGCTAAAAGTGGGTGCAACATCATTGGACTGCGTGGTGGAACTTGTGTTGCCCGCCGGCGAGCCGTTGGTGGCGCTGTGACCCGCTCCAGCGACCCCCTTATATCCATTCCCATACCTGTACCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCAAGGAGTGCCCCCGCGAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAATAAAAATACCAACAGCAAGTTTTAACACTTACCGTTGGTAA", "aa": "MATHSRRLTVTKRALDIRFETNADIAKSGCNIIGLRGGTCVARRRAVGGAVTRSSDPLISIPIPVPIPNPGNIIQHLVKECPREVEPGGETGKRGGEIKIPTASFNTYRW", "aa_hexdigest": "69588fd5fd7208701a1d7b0c2b2ba7aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11887.59829999999, "isoelectric_point": 10.277694892883297 }, "id": "CNOCLNGIAL_1014", "locus": "GCA000143945_01008" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 566188, "stop": 566517, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 265.8, "evalue": 6.8e-29, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "GCTGTCGGTCAGTTGCTGCTCAATGAGTAGAGGAAAGTCCATGCTTGCACTTGCTGGAACGCAAGTAGTGTTTGTGTAAATTGAACAAATAAGATTTAGGACGCTAATAGACGTCACGACTGGACTAGTCTAAGGTCTTTGACTATGATGATGTCCGTAAAGTACCACAGAGACGAGCTTAGTGAAAACTAAGTATGAAACGCGGTAAACTCCACAAGCAAGAAACCTAAATTTTGGTAAGGGAATGTTACTTTAAGAAAAATAATTAAAGTAATGACAGGATTACTGTAGATAAATAACTGACTTTAAAACAGAACATGGCTTATTGGC", "id": "CNOCLNGIAL_41", "locus": "GCA000143945_01009" }, { "type": "tmRNA", "sequence": "contig_1", "start": 566542, "stop": 566928, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 566625, "stop": 566705, "aa": "DKNNDEVLVDPMLIANQQASINYAFA", "nt": "GACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAG" }, "nt": "GGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTGGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA", "id": "CNOCLNGIAL_37", "locus": "GCA000143945_01010" }, { "type": "cds", "sequence": "contig_1", "start": 567019, "stop": 567234, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTCTTTTTGGTGTTATCGGACCAACACTTTTTCCCGCGTACGACAACGCAACTAAAAAGTAATCCTAACCTGTCACTGTTCTTTCGGATTTCTCACAAAGACAACGTTTTTAGTTCGCGCTTAAACCAACTAGGAGCGTTTGCCACCGCTTTATGTATTACGCTTGTGGTGTATGAATTGCCTTTTTTAGGACTGTTTGCCTTTAACTGA", "aa": "MLLFLVLSDQHFFPRTTTQLKSNPNLSLFFRISHKDNVFSSRLNQLGAFATALCITLVVYELPFLGLFAFN", "aa_hexdigest": "5ce299020c964f1922fcedb3f2bebbe7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8125.423799999997, "isoelectric_point": 9.18495578765869 }, "id": "CNOCLNGIAL_1015", "locus": "GCA000143945_01011" }, { "type": "cds", "sequence": "contig_1", "start": 567466, "stop": 567702, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAGATCAACGATAAACTCACCGAAATTAATACCGTTGAATACGAAGTTGCTTCCAAACACAGTCAGTACCTCTTCTACAGCCGTTTTGGTCTGTTAGATACCGCTGCTTACTTTTTGTTCTTGCTGTCTTTTTTTGTGACAGCAGTGATGTTCTTGGTGGGTATCTTTCACACCGAGCAGTTTACCTTAAACGATCAAAACCAGGGCATTAGTGGTTTTTACTTGTTTTGA", "aa": "MSKINDKLTEINTVEYEVASKHSQYLFYSRFGLLDTAAYFLFLLSFFVTAVMFLVGIFHTEQFTLNDQNQGISGFYLF", "aa_hexdigest": "2d8ba9e1b0fd9fff7c4b0f778e406837", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9082.277099999992, "isoelectric_point": 5.006230735778809 }, "id": "CNOCLNGIAL_1016", "locus": "GCA000143945_01012" }, { "type": "cds", "sequence": "contig_1", "start": 567743, "stop": 568807, "strand": "-", "frame": 2, "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "db_xrefs": [ "COG:COG0006", "COG:E", "EC:3.4.11.9", "GO:0004177", "GO:0006508", "GO:0008237", "GO:0046872", "RefSeq:WP_014325558.1", "SO:0001217", "UniParc:UPI0001DD63FB", "UniRef:UniRef100_A0AAX0SPT1", "UniRef:UniRef50_P47566", "UniRef:UniRef90_P75313" ], "nt": "ATGCATAACGAACTGCAACAAAAGCTAGCAGTTTTACACAAACTGCTCCAAGACAATAAAGCTGATGCCATCTTAATTGGCTCAGACCAAAACCGCTTTTGGCTTACTGGTTTTCCCTCCAGTGCTGGTTGGTTAGTGGTTCACAAGCAAAGGGTTAACCTCTTTATTGACGGGCGTTACTTTGAAGCCGCTAAAACGGCAATTGATCCCCTAGTCAAAGTGGAATTATTTACCACTTATAAACAAGTAAAGGCATTATGTGAACAAGTGGGTGTGAAACACCTCTTGATTGAAGGGGATTACCTCACTTTTAACTACCAAAATTTCATTAAGAAGTTGTGTGCGCAGTACACGGTCATTAACGCCCAGGAAATTCGCCGACAAAAGCTCCCGAGTGAAATCTTAGCGATTGAAAAGGTAGTGGAAATTACCCGCAAAGTAGCGGTAAAACTCAAGCGCTTTATCCAACCAGGCATGACGGAGTTGTTCATTGCGCAGTGGATTACTGACCAATTGGTCAAAGCTGGTGGGGCAAAAAACTCGTTTGACCCTATTGTAGCTACTGGTAAGAACGGTGCTAATCCACACCACAAGCCTTCCAAGTTAAAGGTAAAATCAGGTGATTTTGTAACCTGTGACTTTGGCACCATTTACAACGGTTACTGTTCCGATATTACCCGCACCTTCTTAGTTGGTAAAAAACCTAATAATGAAGTGTTGCTCAAGGCCTATAAAAAGGTTGATGAAGCCAATATGGCCGGCATTAACGCTGCTAATACCCAACTCACCGGTGCGGAAGTTGACAAGGTGTGCCGTGACATTATTGAAGCATCGGAGTTCAAAGATTACTTTGTTCACAGCACTGGTCATGGTGTTGGACTTGACATTCACGAAATGCCAAACGTATCAACGTCCTATAACAAGTTGTTGTGTGAAAATGCTGTCATTACGATTGAACCGGGTATTTATATCCCTGGTGTTGGTGGCATCCGCATTGAGGACATGGTGTTAGTTAAAGACCACAAGAGTGTGTGGTTATCAGCCAAGATTCCGCGTGCCTTCTAA", "aa": "MHNELQQKLAVLHKLLQDNKADAILIGSDQNRFWLTGFPSSAGWLVVHKQRVNLFIDGRYFEAAKTAIDPLVKVELFTTYKQVKALCEQVGVKHLLIEGDYLTFNYQNFIKKLCAQYTVINAQEIRRQKLPSEILAIEKVVEITRKVAVKLKRFIQPGMTELFIAQWITDQLVKAGGAKNSFDPIVATGKNGANPHHKPSKLKVKSGDFVTCDFGTIYNGYCSDITRTFLVGKKPNNEVLLKAYKKVDEANMAGINAANTQLTGAEVDKVCRDIIEASEFKDYFVHSTGHGVGLDIHEMPNVSTSYNKLLCENAVITIEPGIYIPGVGGIRIEDMVLVKDHKSVWLSAKIPRAF", "aa_hexdigest": "935fcd33c50928d84f1d840212a7b301", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63FB", "ncbi_nrp_id": "WP_014325558.1", "uniref100_id": "UniRef100_A0AAX0SPT1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63FB", "RefSeq:WP_014325558.1", "UniRef:UniRef100_A0AAX0SPT1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SPT1", "uniref90_id": "UniRef90_P75313" }, "psc": { "uniref90_id": "UniRef90_P75313", "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "ec_ids": [ "3.4.11.9" ], "uniref50_id": "UniRef50_P47566", "cog_id": "COG0006", "cog_category": "E", "go_ids": [ "GO:0004177", "GO:0046872", "GO:0008237", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47566", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47566" ], "product": "Putative Xaa-Pro aminopeptidase" }, "genes": [ "pepP" ], "id": "CNOCLNGIAL_1017", "locus": "GCA000143945_01013" }, { "type": "cds", "sequence": "contig_1", "start": 568987, "stop": 569868, "strand": "-", "frame": 3, "gene": "degV", "product": "DegV domain-containing MG326-like protein", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "RefSeq:WP_010874828.1", "SO:0001217", "UniParc:UPI0000139625", "UniRef:UniRef100_P75312", "UniRef:UniRef50_P75312", "UniRef:UniRef90_P75312" ], "nt": "ATGAAGACCGCGATAATTACTGACTCGACTGCCTCAATTAAGGAAGGAGAGATCCAAGACGTTTACGTGTTACCTTTACAGGTCATTATTAACGGTCAAGACACTTACCGCGATGGTAAGGACATTGACTATGATCGAGTATACCAGTTGTTGAAGGAACACCCCCAAGGGTTAAACATTTCCACATCACTACCACGCCAAGCTGACTTAATTGAGTTAATTGAAGCCATTAAAGACAAGTACGATCGCTTTGTTTTTTTACCTCTCAGTAAGGGTTTGAGTGGTACGTATGACATGATTGTCCAAGCGGTTAAGCCACTCAGTACGCCCAAAAAAGAGTTTGTGGTCTTGGAAACAAGTGACATTGCCATTTCCCTGAAATGGTTAGTACAAGAGGTGAAGGCATTAACTGACACTAACTGCCCAACTAAAGCAATTGCTGAAGTGGTTGATCAGCACAAACAAAGTATTTTTACAGCTGTGACTGTAAAAAACTTAGTGCAGCTACGTAAAGGTGGGCGCATTTCTGGATTAAAGAAAATTATTGCCACACTTTTACGGGTTAAACCGATTATCTTCTTTGATAAAGGGGTAAATACTTTGAGCGGCAAGGCCTTTACCTTTGTCCAAGCATTGGAGAAAATCTTTACCTTTGTCAAATCAAAATTTGGTGATAACTTTAAGATCAAACGGATTGGTTTTTGCAATGCTTTTACCCCAGTTAAGGCCAAGGAAGTTAAAGCTTTAATTTTGGATTTTCTCCACACTAACAAAATCACTTTACAACGTGAAATCGAAAGCTCGTTTATCACAAGTGCCATTATTGCGCATACCGGAATAGATGCGTTTTCGATCAGTTTATTACTCGATAAAAATAAATAA", "aa": "MKTAIITDSTASIKEGEIQDVYVLPLQVIINGQDTYRDGKDIDYDRVYQLLKEHPQGLNISTSLPRQADLIELIEAIKDKYDRFVFLPLSKGLSGTYDMIVQAVKPLSTPKKEFVVLETSDIAISLKWLVQEVKALTDTNCPTKAIAEVVDQHKQSIFTAVTVKNLVQLRKGGRISGLKKIIATLLRVKPIIFFDKGVNTLSGKAFTFVQALEKIFTFVKSKFGDNFKIKRIGFCNAFTPVKAKEVKALILDFLHTNKITLQREIESSFITSAIIAHTGIDAFSISLLLDKNK", "aa_hexdigest": "46556300cca56294085e003b00954d5f", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI0000139625", "ncbi_nrp_id": "WP_010874828.1", "uniref100_id": "UniRef100_P75312", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139625", "RefSeq:WP_010874828.1", "UniRef:UniRef100_P75312" ] }, "ips": { "uniref100_id": "UniRef100_P75312", "uniref90_id": "UniRef90_P75312" }, "psc": { "uniref90_id": "UniRef90_P75312", "gene": "DegV", "product": "DegV domain-containing protein MG326 homolog", "uniref50_id": "UniRef50_P75312", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P75312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75312" ], "product": "DegV domain-containing protein MG326 homolog" }, "genes": [ "degV" ], "id": "CNOCLNGIAL_1018", "locus": "GCA000143945_01014" }, { "type": "cds", "sequence": "contig_1", "start": 569874, "stop": 570680, "strand": "-", "frame": 1, "gene": "menH", "product": "Putative esterase/lipase 2", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0016020", "GO:0052689", "RefSeq:WP_010874829.1", "SO:0001217", "UniParc:UPI000012A1B6", "UniRef:UniRef100_P75311", "UniRef:UniRef50_P75311", "UniRef:UniRef90_P75311" ], "nt": "ATGGCAAGCAGTAAGAGCGATCCCTTTCAATCAATCTTTGCCTTTAAACCACACCGTAAGCGCCATAACTTTATCTTTCTCCATGGTTTTGGCAGTGAGTATTCCAGCTTTAAACACGTGTTTAAACTGTTTGAAAAAAAGCGCTGGTCGTTCTTTGCCTTTAATTTTCCGGGACACGGCAATAATCAGAGTAACAGTGTTGATGAATTGAAGTTGAAACACTATGTTGAATTGGTGTGTGACTTTATCATCCAAAAGCGCTTGAAAAAGGTGGTATTGGTAGGGCACAGTATGGGCGGGGCGATTGCGGTGTTGGTCAATGCTGTATTGCGTGAACGCATTAAGGCCTTAGTGTTGGTAGCACCGATGAATCAAACATCGTTTGTTGTAAGTAAAAAACGGATCTTGGATACCTTGTTTACCAGGAGTCCTAAAAACCAGCAGGACTTCATAGAACACACCGATGATAAGAAGTCGATTGTTAACTTCTTTGTTGGTGCTTTTAAGAAGCGAGTCAACTTTAAAACACTTTACAGTGACATGGTGCAAAACGCCAAGTATGGTAATGATTATCTCGAAGCGGGTTACAACGCAATTAAGGACAAACCCACCTTAGTGGTTCTCGGTTCCAATGATATTGTCACCCCCACAAAGGCTTCTGTGGAATACTTAGCTAAACACAGCGAAACGATCATTTTCAAAATTATTGATGGTGTAGGACATTCACCCCACTACTATGCACCCAAACTTTTTTTTGATTACATTGGGGAGTTTCTAGATAATATAAAGCGAAATAAGGACAAATAG", "aa": "MASSKSDPFQSIFAFKPHRKRHNFIFLHGFGSEYSSFKHVFKLFEKKRWSFFAFNFPGHGNNQSNSVDELKLKHYVELVCDFIIQKRLKKVVLVGHSMGGAIAVLVNAVLRERIKALVLVAPMNQTSFVVSKKRILDTLFTRSPKNQQDFIEHTDDKKSIVNFFVGAFKKRVNFKTLYSDMVQNAKYGNDYLEAGYNAIKDKPTLVVLGSNDIVTPTKASVEYLAKHSETIIFKIIDGVGHSPHYYAPKLFFDYIGEFLDNIKRNKDK", "aa_hexdigest": "77a8a3ac89beaa0abf5b2a128db2a15a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012A1B6", "ncbi_nrp_id": "WP_010874829.1", "uniref100_id": "UniRef100_P75311", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012A1B6", "RefSeq:WP_010874829.1", "UniRef:UniRef100_P75311" ] }, "ips": { "uniref100_id": "UniRef100_P75311", "uniref90_id": "UniRef90_P75311" }, "psc": { "uniref90_id": "UniRef90_P75311", "gene": "MenH", "product": "Putative esterase/lipase 2", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75311", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0016020", "GO:0052689" ] }, "pscc": { "uniref50_id": "UniRef50_P75311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75311" ], "product": "Putative esterase/lipase 2" }, "genes": [ "menH" ], "id": "CNOCLNGIAL_1019", "locus": "GCA000143945_01015" }, { "type": "cds", "sequence": "contig_1", "start": 570667, "stop": 574089, "strand": "-", "frame": 3, "gene": "smc", "product": "putative MG328-like protein", "db_xrefs": [ "COG:COG1196", "COG:D", "RefSeq:WP_014574996.1", "SO:0001217", "UniParc:UPI0001DD63FC", "UniRef:UniRef100_A0AAX0S3B7", "UniRef:UniRef50_P75310", "UniRef:UniRef90_P75310" ], "nt": "ATGACAGCAGAAAAAGAGCTAGGTATCCACGATTTTGACGAAAACTTAAACGAACAAAGTTTGCTCAAGCAACTCATGCCCAAAACCCGTAACATTACCCTAGAACCAACAAGGGAAACAATAACAGTTCCCCTAGAACGTAACATTGTAGAACGTAGTTTCTTTGGCGAATTAGATCAGTTAGAGAAAGAACAATCAATTCGCAATTTTTTTCACTTTATGGTAGATATGGGTGTGGTCAAAAGCCAACCCCATGTTGATGTGTTAAACCACTTCGCTAACCAACTCCAAGTTCATAAACCGCAAACCGTTGATCTAACAATGGAGTTTTTAGAACAAGAAGGCCAGGAAGTACTAACAAAGGAAATTAAGGCTGGTTTTTGTGAAATTACCCCCTCTAGTATTACCGAACAAACGACCAAGCCCCAGTTAGATGAAACTCAACTAGTCGATGAATACGTGCACACCAAAGAGTTAGAAACAACACCAATACCTATATCCTTTGCCACTAAGGAAGTACTGTTTGAAGAGGTCTTTAATACTCCTTCAACCCAACAAGTCGATGAAAGTGTGCTTGTCAACGAATACATTGAACTAACCCAACAAATCAAAAACGCATCAGAACAAGTTTCATCAAACCATACCCATCAGTTTAGTGTGGCAACAGAACCAGCAGCCACAAAAGCTGTTAGTGAAACTATGTTGTTGGATGACTATGTCGAAATGGTGGAACAGGATGTTCAAGCCCAAACTGCTTTACCCCAAGCAGCACTTGATCCTACTGTTAGCTTGACCTTTAGCAGTCCGATTGACAGTAATGCCATTTTGGTGTACCCGGAAATGAAGGTACCTCATGTATTTGACACAGTTGCACCAACTACCACAACTGTTCCGTTAGACCAAACACAACTGCTTGACGAACTAGTGGAAGTGCCAGTTTTAACTCACACTGTAACTCCAGCACCGTTACAACCAAAAGCAGCACCAACTAACTTTGCACTTGACCAAACCCAGTTAGTCGATGAATTAGTAACAGTACCACTCACCCATACATTAGTCAACGAGTCTGCACCAGTTACACCTGTTGTTGTGACATCACCTGCAGCTGAACATAGCTTCTCGATTACAACGGTGGACAAGGCTAATTTAACTAATGCTTTGAGCCAAACAGTGGTTATAAAACCCGCTGAAGACAGCGCTCATCAAAGTGCCGTTTTAGACAAGGAAATTGCCACCAAACAAGTGCAGTTACAACAATTGCAAGCCCAAATTGAACTACGCCAAGCTCAACTAGAAACACCTCCTGTAACTTACATGGGTGTGGAAGAATACAAGCTACTGCCTGTTCAAGATGTAGTGCCAGTACAACCTACCGTGAGTTTTGAAATGACCCTGCTCCAAGAACAGTTAGATAAGGCCTTGAAGCACAACGCTGCTTTACAAATCCAGCTAGAAGAGCAGTTAGCAAAGCCACTTCAGTACGACCAAAGTCCGGTGTTACAGGAACGGATTGAACTGTTGCAAAACCAAAATACTAACCTCACTCAGGAGTTGAATGAACTGCAACAAAAGCTGTTTAAGAGCCAAAACAACAGCTTACTCTTAGCACGTTTGGAAGAGGAAAACCGTACGCTTAAACAGCATTTACAAAACAACCTACCGGAAGCTAACCAGTTAAACTTTGTCTTGGAAAAACAGCTTGAACAACTCCAACAAGACAAACACAGCTTAACACTCCAAATCGAACAGTACAAGTTTGATTCCAAAAAACACCAGGAACAACTGGCCTTAATTCCGTCGTTACGCAGTGAAATTAACAGTTTGGAAACGGAAGTAATTAGCTTAAAGCAAACTAACCAACGCTTAAGCCTAATTGAGCGTGAAAACAACTTCCTCAAAACCGAAATTAAACAGCTACGTGAAACTAAGTTAAACGATGAAAACACCAAGTACCGTAATCTTTTAAAGCAGTATGAGTTAATGCGTGCTGACAGTGATGCTAAGTTAAAGGAATTGGAACACGAACAACACCTAGCACACCAACACCACCAGGAACAGTTAGCACAATTACAACGCCACAACGAAGCTTTAGTAAAAGAGTTAGACCAAGTTAAGGCCACTAACTTTGAGTTAGGTCTCGCAGCACAAGGTTTTGAACAGCAAAAAGTGGTACTAGAACAAAAAAACAGCAGTTTACTTGCAAGCTTACAAGCAGCTGAGGAAAACGTCCATGCCTTGGGTATTACTAACAGTGAACTACAAAACCAACTAAATGTTTTAGAATTTACCCACAAGGAAAAAACTGCGTTTGACAGCAAAACGCTAACACTCACAAAGCAACAGCTAGAACAAACCCAGTTTGACCTTAGCTTAACCCAAGAACAGTTAGCTACCTTCAAACAACAAAACCAAAGCCTAACTGACAAACTAATGGCTAGCGAAACCCAGTTAAACCACTTGCAACAGAGTGATGAAAACTTAACCCAGCTCCAAACGCAGCACGAGCTGTTGCAAGAGAGTTACAACAAATTGCAAGATGAAGCTAATCACACCCAGCAACAATTCCATCAAGCACAAAATGAACTTGATGCTGCCCACCAACAGTTAGCACTCTTTAAGCAAAACAACGAGGAATTAACTGATAAATGCAGTAACATTCAAAATGAGTTGCATGATTTAAACCGGGTAAAGACTAACTGGGAGAACCTCAATACAGAGCATAACTTGTTGCAAGACAAATATGCTCAACAAAAAGAGCAAATGCAACATGAACACAGTAATTTAGCCCAAATCCAAGCGGAGCATGAGTTACTGCAAGAGAGTTACAACAAGGTCAAAGCAGAATTAAACGAAATTCAAATTACTAACCTTAATGAGGCCAACGCCCAGTACCAGGACTTGCTCTCCGCTTACGAGTTGTTACAAAGTAATCACAACAAGCTCAAGCAAGAGTTGCAAGTTTTAAACCAGGTCAATTTGGAAAAACAACAGTTAGCGCAAAAGTTACACAACACACACCAAAGTTTAAGTCAAACCCATGCAGAGCTAACCCAACTCCAAGCTGCTTACAATAACCTCCAAGCAACTCCACCGGTAAGCGATGAATTGTTAGAACAATTTAACCAAGTGCAGCTAGAAAAACAACGCTTACTGCAACAAAACTTAGCCTTAGTACACGAGTTGCAATACTTCAACGAACTCAACTCGAGTCAAACCCACGAAATTAAAACAAAGCAAGACGAAACTGTTAAAGAGGTCATTATTGTGGAAAAGGAAATTCCAGTGCTACCCGAAAAGAAACCACGGCTAAAAAAACGTGATATTGTGATCGAAAACAAGGAAGATGCTTTGGGCAAGTTATCCAAAAAGGAACGGATCCAAGCGTACGCTGAACGCCTAGCTAAGATCAATGGCAAGCAGTAA", "aa": "MTAEKELGIHDFDENLNEQSLLKQLMPKTRNITLEPTRETITVPLERNIVERSFFGELDQLEKEQSIRNFFHFMVDMGVVKSQPHVDVLNHFANQLQVHKPQTVDLTMEFLEQEGQEVLTKEIKAGFCEITPSSITEQTTKPQLDETQLVDEYVHTKELETTPIPISFATKEVLFEEVFNTPSTQQVDESVLVNEYIELTQQIKNASEQVSSNHTHQFSVATEPAATKAVSETMLLDDYVEMVEQDVQAQTALPQAALDPTVSLTFSSPIDSNAILVYPEMKVPHVFDTVAPTTTTVPLDQTQLLDELVEVPVLTHTVTPAPLQPKAAPTNFALDQTQLVDELVTVPLTHTLVNESAPVTPVVVTSPAAEHSFSITTVDKANLTNALSQTVVIKPAEDSAHQSAVLDKEIATKQVQLQQLQAQIELRQAQLETPPVTYMGVEEYKLLPVQDVVPVQPTVSFEMTLLQEQLDKALKHNAALQIQLEEQLAKPLQYDQSPVLQERIELLQNQNTNLTQELNELQQKLFKSQNNSLLLARLEEENRTLKQHLQNNLPEANQLNFVLEKQLEQLQQDKHSLTLQIEQYKFDSKKHQEQLALIPSLRSEINSLETEVISLKQTNQRLSLIERENNFLKTEIKQLRETKLNDENTKYRNLLKQYELMRADSDAKLKELEHEQHLAHQHHQEQLAQLQRHNEALVKELDQVKATNFELGLAAQGFEQQKVVLEQKNSSLLASLQAAEENVHALGITNSELQNQLNVLEFTHKEKTAFDSKTLTLTKQQLEQTQFDLSLTQEQLATFKQQNQSLTDKLMASETQLNHLQQSDENLTQLQTQHELLQESYNKLQDEANHTQQQFHQAQNELDAAHQQLALFKQNNEELTDKCSNIQNELHDLNRVKTNWENLNTEHNLLQDKYAQQKEQMQHEHSNLAQIQAEHELLQESYNKVKAELNEIQITNLNEANAQYQDLLSAYELLQSNHNKLKQELQVLNQVNLEKQQLAQKLHNTHQSLSQTHAELTQLQAAYNNLQATPPVSDELLEQFNQVQLEKQRLLQQNLALVHELQYFNELNSSQTHEIKTKQDETVKEVIIVEKEIPVLPEKKPRLKKRDIVIENKEDALGKLSKKERIQAYAERLAKINGKQ", "aa_hexdigest": "315cdd9ace9c2b83a954a0d547d1a3f2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63FC", "ncbi_nrp_id": "WP_014574996.1", "uniref100_id": "UniRef100_A0AAX0S3B7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63FC", "RefSeq:WP_014574996.1", "UniRef:UniRef100_A0AAX0S3B7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S3B7", "uniref90_id": "UniRef90_P75310" }, "psc": { "uniref90_id": "UniRef90_P75310", "gene": "Smc", "product": "Uncharacterized protein MG328 homolog", "uniref50_id": "UniRef50_P75310", "cog_id": "COG1196", "cog_category": "D" }, "pscc": { "uniref50_id": "UniRef50_P75310", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75310" ], "product": "Uncharacterized protein MG328 homolog" }, "genes": [ "smc" ], "id": "CNOCLNGIAL_1020", "locus": "GCA000143945_01016" }, { "type": "cds", "sequence": "contig_1", "start": 574126, "stop": 574548, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14714.11" ], "nt": "ATGGCTAAGTTTAAAAAGCAGTTACAAAGTCAGTTTCAACACCTCAGCTTTGCTCCCATTGTTTTTATTAGTGTCAAAAACAACAAGCGCTTACATACCATCTTTGAACAGCTCCAAATTATCCAGGAACAGCTCACCAAAAAGATTTCAACTTCCCTGTTAAACGATGTCATCCAACAAGCACAACTGTTTAACCAAGCACCCCTGTTTAAGGGTGGGAGGTTACAGGTAACTTATGCGGTGCAAACTCACAGTCAAACACCGCACTTTGTACTGTTCTGTAATGACCCTAAGTTTGTCCACTTTTCCTATGCGCGCTTTTTAGAGAACAAGATTAGGGAAAGCTTTGGTTTTAGTGCTGTACCTATTACTCTTTATTTCAAGTCCAAAAATGCACGGATAAGAGGTGTCGCAAAAACCTAA", "aa": "MAKFKKQLQSQFQHLSFAPIVFISVKNNKRLHTIFEQLQIIQEQLTKKISTSLLNDVIQQAQLFNQAPLFKGGRLQVTYAVQTHSQTPHFVLFCNDPKFVHFSYARFLENKIRESFGFSAVPITLYFKSKNARIRGVAKT", "aa_hexdigest": "baa0285819b27dedec0c8312a740e54d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14714.11", "name": "KH_dom-like", "length": 81, "aa_cov": 0.5785714285714286, "hmm_cov": 1.0, "evalue": 3.5294995296793716e-31, "score": 102.84827423095703, "start": 48, "stop": 128 } ], "seq_stats": { "molecular_weight": 16233.708699999988, "isoelectric_point": 10.332235145568848 }, "id": "CNOCLNGIAL_1021", "locus": "GCA000143945_01017" }, { "type": "cds", "sequence": "contig_1", "start": 574576, "stop": 575475, "strand": "-", "frame": 3, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "SO:0001217", "UniRef:UniRef50_P75309", "UniRef:UniRef90_P75309" ], "nt": "GTGTTTACTGTTGCCATTATCGGCCGACCTAACGTTGGCAAATCGAGTTTGTTCAATCGCTTAATCCAAAAACCCTACGCGATTATTTCCGATACGCCCAATACCACAAGGGACCGCATTTATGGTGTTGGTGAATGGTTAACGCGGCAAATTGCCTTTATTGATACTGGGGGATTAATTTCCCAAAAGACCCCGCTCCAACAACAAATTGAAGTCCAAGTTCGAGCAGCCTTAAGTCAAGCTAATGCGATTATCTTCTTGGTGTCTTACCAAGAACAGATTAGCAGTGATGACTTTTATGTAGCTAAGGTATTGAAAAAGATTAAGGACAAACCGATTTTGTTGGTCGTCAATAAATCAGAAAACCTCAAACCAGATGCTTATGAACCCAACCTACAACAGTTCTACAGCTTTGGGTTTGGCCAACCCGTTTGTGTGAGTGCTAGCCATGGCATTGGCATTGGTAACCTAATGGATCGACTAGTTAAGGACAACCAACTCCCCCCTTACCATGGTAGTAGTGAAACCAATCCCGAAGTGCGCTTTTGTGTAATTGGTAAACCCAATGTTGGCAAGAGTTCTTTAATTAACCAACTGGTGCAGCAAAACCGGGTGTTAGTGTCCGATGAGAGTGGTACTACCCGTGATGCAATTGATATTCCCTTAAGGGTTAATGGGCAAAACTACCTTTTAATTGATACGGCTGGGATTCGCCGTAAGGGGAAAATAGCCCCCGGAATTGAAGCGGCCTCGTATGGGAAAACCCAGTTAGCAATCGCTAGGAGCAACATTATTCTGTTAATGGTTGATGGTTCCAAACCGTTGAGTGAACAGGACGAGATTATCGGTGGATTAGCCCAAGCCGCTTTAATTCCGGTGATTATTCTGGTCAACAAATGA", "aa": "MFTVAIIGRPNVGKSSLFNRLIQKPYAIISDTPNTTRDRIYGVGEWLTRQIAFIDTGGLISQKTPLQQQIEVQVRAALSQANAIIFLVSYQEQISSDDFYVAKVLKKIKDKPILLVVNKSENLKPDAYEPNLQQFYSFGFGQPVCVSASHGIGIGNLMDRLVKDNQLPPYHGSSETNPEVRFCVIGKPNVGKSSLINQLVQQNRVLVSDESGTTRDAIDIPLRVNGQNYLLIDTAGIRRKGKIAPGIEAASYGKTQLAIARSNIILLMVDGSKPLSEQDEIIGGLAQAALIPVIILVNK", "aa_hexdigest": "5ea8883a405ca73f4a52e9ab9e63f8cc", "start_type": "GTG", "rbs_motif": null, "genes": [ "der" ], "truncated": "5-prime", "pseudogene": { "start": 574273, "stop": 575475, "observations": { "mutation": [ 574578 ], "stop": [ 574578 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75309", "query_cov": 1.0, "subject_cov": 0.6659242761692651, "identity": 1.0, "score": 582.0, "evalue": 1.92e-207, "gene_start": 1, "gene_end": 299, "reference_start": 1, "reference_end": 299, "reference_sequence": "MFTVAIIGRPNVGKSSLFNRLIQKPYAIISDTPNTTRDRIYGVGEWLTRQIAFIDTGGLISQKTPLQQQIEVQVRAALSQANAIIFLVSYQEQISSDDFYVAKVLKKIKDKPILLVVNKSENLKPDAYEPNLQQFYSFGFGQPVCVSASHGIGIGNLMDRLVKDNQLPPYHGSSETNPEVRFCVIGKPNVGKSSLINQLVQQNRVLVSDESGTTRDAIDIPLRVNGQNYLLIDTAGIRRKGKIAPGIEAASYGKTQLAIARSNIILLMVDGSKPLSEQDEIIGGLAQAALIPVIILVNKWDLVQKDSNTMAKFKKQLQSQFQHLSFAPIVFISVKNNKRLHTIFEQLQIIQEQLTKKISTSLLNDVIQQAQLFNQAPLFKGGRLQVTYAVQTHSQTPHFVLFCNDPKFVHFSYARFLENKIRESFGFSAVPITLYFKSKNARIRGVAKT" }, "paralog": false, "identity": 0.995, "score": 773.0, "evalue": 4.21e-285, "description": "Internal stop codon at 574578. Nonsense mutation around 574578.", "psc": { "uniref90_id": "UniRef90_P75309", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75309", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75309", "EC:3.6.5.-", "UniRef:UniRef50_P75309", "COG:COG1160", "COG:J", "KEGG:K03977", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P75309", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75309" ], "product": "GTPase Der" } }, "id": "CNOCLNGIAL_1022", "locus": "GCA000143945_01018" }, { "type": "cds", "sequence": "contig_1", "start": 575477, "stop": 576130, "strand": "-", "frame": 2, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "COG:COG0283", "COG:F", "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431", "RefSeq:WP_010874832.1", "SO:0001217", "UniParc:UPI000012DCF0", "UniRef:UniRef100_P75308", "UniRef:UniRef50_P47572", "UniRef:UniRef90_P75308" ], "nt": "ATGTATTTTCAAATTGCCATTGACGGTCCTAGTAGTTCTGGTAAATCTAGTGTTGCTAAAACAGTGGCCCGACAGCTCGGTTTTGAGTACTTTTCCACTGGCAAAATGTACCGGGCCTTCGCTTACGTGATGCAGGTCAACCGCTTGGATGTCAACCTTTTGTTAAAGGTGATTAACCAAATTAACTGGCGCTTTGAACACGAAAAGGTGTTTTATAACAACGCTGATATCTCAGAGGTTATCCTAAACCAGGAAATTGCCCAACTAGCGAGCAATTTAGCAACCAATCCCGAAGTGCGTAAGATGGCGGTATTGCGCCAACAAGCGTTAGCTAAAAACACCAACATTGTGATGGATGGACGCGACATTGGTACCGTTGTATTGAAGGACGCGCAGTTAAAGTACTTCTTGGATGCTAAACCGGAAATAAGAGCGCAACGACGCGCCCAAGATCTTGGCATAGCATATGACAGTGACAAGGCCTTTCAGGAACTAGTAGCGGAAATTAAGCACCGTGATGCGGTGGATACTTCCAGAACTGCTGATCCCTTAGTGCAAGCCCCCGATGCGATTTACATCGACAGTTCTAACCTTACTTTTCAGCAAGTAGTCGAGTTAATGGTGCAACAAGCGCGTACAGTTTTTAAACTTTAA", "aa": "MYFQIAIDGPSSSGKSSVAKTVARQLGFEYFSTGKMYRAFAYVMQVNRLDVNLLLKVINQINWRFEHEKVFYNNADISEVILNQEIAQLASNLATNPEVRKMAVLRQQALAKNTNIVMDGRDIGTVVLKDAQLKYFLDAKPEIRAQRRAQDLGIAYDSDKAFQELVAEIKHRDAVDTSRTADPLVQAPDAIYIDSSNLTFQQVVELMVQQARTVFKL", "aa_hexdigest": "2ab2763f60287f23f9a9edc9ce80afdf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012DCF0", "ncbi_nrp_id": "WP_010874832.1", "uniref100_id": "UniRef100_P75308", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DCF0", "RefSeq:WP_010874832.1", "UniRef:UniRef100_P75308" ] }, "ips": { "uniref100_id": "UniRef100_P75308", "uniref90_id": "UniRef90_P75308" }, "psc": { "uniref90_id": "UniRef90_P75308", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_P47572", "cog_id": "COG0283", "cog_category": "F", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ] }, "pscc": { "uniref50_id": "UniRef50_P47572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47572" ], "product": "Cytidylate kinase" }, "genes": [ "cmk" ], "id": "CNOCLNGIAL_1023", "locus": "GCA000143945_01019" }, { "type": "cds", "sequence": "contig_1", "start": 576157, "stop": 576666, "strand": "-", "frame": 3, "gene": null, "product": "putative MG331-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75307", "UniRef:UniRef90_P75307" ], "nt": "TTGAGCCCGAAAATTCTGTCGCACTACCAGCCTTTTAGTGAATTGAGTGCTAATCCGGTGAAACGCAAAAGTGAACCCAATCAGGTCTTACAATCACTGCAAACTCTCATTAACAACTTCCAAACATCGGATTTTCAAGCCATCGCCACCCAAGCTGATGCCTTGTGGTTACAAATTAAACACCACGATAGCGCGACTGGTTATGAAAGTTGGATTACTGATGACCGGGGGATGGATAAAATAGATAACCTAAAGCGCTTTTTTGAGGACAACGAACAACAGTTTATTCAGCAAACGCAAACTTTCGTAGTGCAATTGGGGAACTATAATAAGGCACTAATGGCACTCACTAACGAAAAAGTGAGCTTTACCACCACTGATTTAAGCCAGCCCTTAGCGAGCAGTTACAACCCGTTATTTAAGCGGATTTTGCTCAGTTTATTAGTATTCTTTATTAGTAGTTTAGTGGGTTTGGTTGTTTTACTAACCCTCTTATTCACTTCCCTTTAA", "aa": "MSPKILSHYQPFSELSANPVKRKSEPNQVLQSLQTLINNFQTSDFQAIATQADALWLQIKHHDSATGYESWITDDRGMDKIDNLKRFFEDNEQQFIQQTQTFVVQLGNYNKALMALTNEKVSFTTTDLSQPLASSYNPLFKRILLSLLVFFISSLVGLVVLLTLLFTSL", "aa_hexdigest": "b5b40653b8a922273dadba5fbd55fb84", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75307", "query_cov": 1.0, "subject_cov": 0.8086124401913876, "identity": 0.9940000000000001, "score": 315.0, "evalue": 9.49e-108, "valid": true, "product": "Uncharacterized protein MG331 homolog", "uniref50_id": "UniRef50_P75307", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P75307", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75307" ], "product": "Uncharacterized protein MG331 homolog" }, "genes": [], "id": "CNOCLNGIAL_1024", "locus": "GCA000143945_01020" }, { "type": "cds", "sequence": "contig_1", "start": 576685, "stop": 576786, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACGCTTAGAGAAGTTTCGCTTTTACCGCCAGAACGATGACAATAATACGACTGTTCAAAAAGCGTTACTGAAAGCGCAAAAACAGGTCGCTAGTTGA", "aa": "MGRLEKFRFYRQNDDNNTTVQKALLKAQKQVAS", "aa_hexdigest": "cd0a4e426ba0cf40f9ec792e15d2b977", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3899.396700000001, "isoelectric_point": 10.160878181457516 }, "id": "CNOCLNGIAL_1025", "locus": "GCA000143945_01021" }, { "type": "cds", "sequence": "contig_1", "start": 576789, "stop": 577496, "strand": "-", "frame": 1, "gene": "tACO1", "product": "putative transcriptional regulatory protein MPN_478", "db_xrefs": [ "COG:COG0217", "COG:KJ", "GO:0003677", "GO:0005829", "GO:0006355", "RefSeq:WP_010874834.1", "SO:0001217", "UniParc:UPI000013963C", "UniRef:UniRef100_P75306", "UniRef:UniRef50_P47574", "UniRef:UniRef90_P75306" ], "nt": "ATGCCGCGGAAGCATTTAATTGCCAGTCAAACAAACAAAAAACAACAATCCAACGCGAAGCAACTACAAAAGTTAGCGAAGCGGATCGCTGCTGCTGTCAAAAAAGGTGGCAGTAACATTGATGCCAACCCCCAGCTAAAGGTGGCAGTCGAACTAGCGTTAGCTCATGGTTTAAGTGCGGATTCAATTAAACGCAACATCCATGGCAGTGAAAAGGATCCCACAAAACTAAGCGAGTTTTGCTATGAGATCTTTGGTCCCAATGGTGTTGGCATCATTGTCTTTGGTCTAACCGATAATCCGAACCGGTTGTTGAGTAGTTTAAACGGTTACATTGCTAAATTAAAGGCACAGCTAGCCAAGCCCAACAGTGTTAAAATTAACTTTGAGGAAAAGGGCATTGCGCTAGTTAAACACAATAACTTTACCCAAGACGAGTTAATTGAACTGTTAATTAGTAACAACATTAATTTGTTAGATCTCAATGAAGATGATGACAGCTTTGAAGTAGTGGTCGATTCACCGAGTTACTTTGCGTTGAAGGATTTACTCGTTAAAAACAGTTTTACGATTGAAGCGAGTGAGTTGCGCTTAATTCCGCTGTTAACGGTGGAACTTAACGCCGAACAACACACCCTCTTAAACCGCTTTTTAAATGCGTGTGAAGAGGATGATGACATCCAAACAGTAGTGCATAATGCACTGTAG", "aa": "MPRKHLIASQTNKKQQSNAKQLQKLAKRIAAAVKKGGSNIDANPQLKVAVELALAHGLSADSIKRNIHGSEKDPTKLSEFCYEIFGPNGVGIIVFGLTDNPNRLLSSLNGYIAKLKAQLAKPNSVKINFEEKGIALVKHNNFTQDELIELLISNNINLLDLNEDDDSFEVVVDSPSYFALKDLLVKNSFTIEASELRLIPLLTVELNAEQHTLLNRFLNACEEDDDIQTVVHNAL", "aa_hexdigest": "94cd58cb630adeb32042c4dea2725291", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013963C", "ncbi_nrp_id": "WP_010874834.1", "uniref100_id": "UniRef100_P75306", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013963C", "RefSeq:WP_010874834.1", "UniRef:UniRef100_P75306" ] }, "ips": { "uniref100_id": "UniRef100_P75306", "uniref90_id": "UniRef90_P75306" }, "psc": { "uniref90_id": "UniRef90_P75306", "gene": "TACO1", "product": "Probable transcriptional regulatory protein MPN_478", "uniref50_id": "UniRef50_P47574", "cog_id": "COG0217", "cog_category": "KJ", "go_ids": [ "GO:0005829", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47574", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47574" ], "product": "Probable transcriptional regulatory protein MG332" }, "genes": [ "tACO1" ], "id": "CNOCLNGIAL_1026", "locus": "GCA000143945_01022" }, { "type": "cds", "sequence": "contig_1", "start": 577674, "stop": 578093, "strand": "-", "frame": 1, "gene": "azoR", "product": "FMN-dependent NADH:quinone oxidoreductase", "db_xrefs": [ "COG:C", "COG:COG1182", "EC:1.6.5.-", "GO:0009055", "GO:0010181", "GO:0016652", "GO:0016655", "KEGG:K01118", "SO:0001217", "UniRef:UniRef50_P47575", "UniRef:UniRef90_P75305" ], "nt": "ATGAAGAAGGTAATTATTATTGATGCTTCTGTATCACCTAGTGGTTCTTACACCCATTTATTGTTAGAACGCTTTTTAGCTACCTTTCAAGCTAAAAACAAGGACGTAGAGCTCTCCACTTGGAACCTCAACGAACTACCAGTGGGACAAATTTCCTATAACACCCAAAACGCTGGAACTTTCTTTTCTGTAGAAAACTCCGATAAGTACATTGATGCGCTCAAAGCAGCGCACGGTGTTATTATCTTGGCGCCAATGACCAACTTTAACTACCCCGCAACGTTAAAGAACTTTATTGACCATGTCTTTGTGGCAAACAAAACCTTTAAGGATAAGTATGTCACTAAGGGCGCATCATCAGGAATGTTGGGTAACCTCAAGGTAGTCGTTTTGGGTTCCCAAGGCGCACCGTTAGGTTGA", "aa": "MKKVIIIDASVSPSGSYTHLLLERFLATFQAKNKDVELSTWNLNELPVGQISYNTQNAGTFFSVENSDKYIDALKAAHGVIILAPMTNFNYPATLKNFIDHVFVANKTFKDKYVTKGASSGMLGNLKVVVLGSQGAPLG", "aa_hexdigest": "c15fd1d27fece49966a775d820ee2fcf", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "azoR" ], "truncated": "5-prime", "pseudogene": { "start": 577500, "stop": 578093, "observations": { "mutation": [ 577676 ], "stop": [ 577676 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75305", "query_cov": 1.0, "subject_cov": 0.7055837563451777, "identity": 1.0, "score": 275.0, "evalue": 6.42e-93, "gene_start": 1, "gene_end": 139, "reference_start": 1, "reference_end": 139, "reference_sequence": "MKKVIIIDASVSPSGSYTHLLLERFLATFQAKNKDVELSTWNLNELPVGQISYNTQNAGTFFSVENSDKYIDALKAAHGVIILAPMTNFNYPATLKNFIDHVFVANKTFKDKYVTKGASSGMLGNLKVVVLGSQGAPLGWYPWGDHVNSLRGLFGFAGVASFASVIIDGTKLLYKDKSKSEVVDMFAKQVDAIANEF" }, "paralog": false, "identity": 0.9949238578680203, "score": 388.0, "evalue": 9.69e-140, "description": "Internal stop codon at 577676. Nonsense mutation around 577676.", "psc": { "uniref90_id": "UniRef90_P75305", "gene": "azoR", "product": "FMN-dependent NADH:quinone oxidoreductase", "ec_ids": [ "1.6.5.-" ], "uniref50_id": "UniRef50_P47575", "cog_id": "COG1182", "cog_category": "C", "kegg_orthology_id": "K01118", "go_ids": [ "GO:0009055", "GO:0010181", "GO:0016652", "GO:0016655" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75305", "EC:1.6.5.-", "UniRef:UniRef50_P47575", "COG:COG1182", "COG:C", "KEGG:K01118", "GO:0009055", "GO:0010181", "GO:0016652", "GO:0016655" ] }, "pscc": { "uniref50_id": "UniRef50_P47575", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47575" ], "product": "FMN-dependent NADH:quinone oxidoreductase" } }, "id": "CNOCLNGIAL_1027", "locus": "GCA000143945_01023" }, { "type": "cds", "sequence": "contig_1", "start": 578121, "stop": 578639, "strand": "-", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6", "UniRef:UniRef90_UPI001EFE51D6" ], "nt": "ATGCTAAGTACCGTTACTCCCTTCTTGGCGGAACGGATCTACCAACAGTTTCACAGTGGCAGTGTCATGCAAGCAAGTTGGCCAACTGCCAAAGCAGTTAAACCACCGAAACTGTTTGCTGATGTTGTTGAAGCCGTGTCCAGCTTGCGCCACTACAAGGCCAACAACCAACTAGTAGCTAACCAGAATTTAACCGTTGTTTTAAGTGGTAAAGCTGCTCCTGTAGTGCAAAACTACTTCCACTTTAACTGGGTCGATCTTAGGATAGAAGTTAACAAAACTCCAGGCTTCCAAATCAAAATCGTTGACAATGCAGCCAACAACTTAGCCCACTTAGAAAAACAACGGAGCTTTTATTTAGCGGAAGTCCAACGTAGTCAAGCGATTAACACTAACCCTGCCTTTCTAAAAAAAGCACCACCGCACAAAGTTAAGGCGGAGCTTTTAAAGTTAGAGGAATATCAAAAAAAATTAGCGGAAGTCAATCATTTAATCGCTAAATTAACTAAAGCTGAGTAG", "aa": "MLSTVTPFLAERIYQQFHSGSVMQASWPTAKAVKPPKLFADVVEAVSSLRHYKANNQLVANQNLTVVLSGKAAPVVQNYFHFNWVDLRIEVNKTPGFQIKIVDNAANNLAHLEKQRSFYLAEVQRSQAINTNPAFLKKAPPHKVKAELLKLEEYQKKLAEVNHLIAKLTKAE", "aa_hexdigest": "7a8e46fe17aab007175f7e64626681e5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 578121, "stop": 578939, "observations": { "mutation": [ 578747 ], "stop": [ 578747 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFE51D6", "query_cov": 1.0, "subject_cov": 0.46112600536193027, "identity": 0.988, "score": 332.0, "evalue": 5.55e-112, "gene_start": 1, "gene_end": 172, "reference_start": 202, "reference_end": 373, "reference_sequence": "MPTDLLVTGYDILFFWVLRMTFNSYFQTKQLPFKQVLIHGLVRDAQNRKMSKSLNNGINPMDLIRDYGADATRLFLTSNHTPGDDLIFNEQKLKSAANFLNKLWNVTKYVLQLGEQAKTVPSTHLPSTLSERWIWAKLKQLIVQTTKLLDKYQLALANQALVNFIWNDFCNTFIETIKQEDTALLPQLYTTAKTVLSTAVVMLSTVTPFLAERIYQQFHSGSVMQASWPTAKAVKPPKLFADVVEAVSSLRHYKANNQLVANQNLAVVLSGKAAPVVQNYFHFNWVDLRIEVNKTPGFQIKIVDNAANNLAHLEKQRSFYLAEVQRSQAITTNPAFLKKAPPHKVKAELLKLEEYQKKLAEVNHLIAKLTKAE" }, "paralog": false, "identity": 0.9889705882352942, "score": 529.0, "evalue": 5.7e-192, "description": "Internal stop codon at 578747. Nonsense mutation around 578747.", "psc": { "uniref90_id": "UniRef90_UPI001EFE51D6", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFE51D6", "UniRef:UniRef50_UPI001EFE51D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6" ], "product": "class I tRNA ligase family protein" } }, "id": "CNOCLNGIAL_1028", "locus": "GCA000143945_01024" }, { "type": "cds", "sequence": "contig_1", "start": 578745, "stop": 579242, "strand": "-", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6", "UniRef:UniRef90_UPI001EFE51D6" ], "nt": "GTGCCAACTGATCTTTTGGTAACCGGGTATGACATCCTCTTCTTTTGGGTCTTACGGATGACCTTTAACTCGTACTTTCAAACCAAGCAGTTACCTTTTAAACAAGTGTTAATTCACGGTCTAGTCCGCGATGCCCAAAACCGCAAAATGTCCAAGTCCCTAAACAACGGGATTAACCCAATGGACTTAATCCGTGATTATGGGGCCGATGCCACGCGCTTATTCTTAACTTCCAATCACACTCCGGGTGATGATTTAATCTTCAACGAACAGAAGTTAAAGAGTGCCGCTAACTTCTTGAACAAGTTGTGGAATGTGACCAAGTACGTGTTGCAGTTAGGAGAACAAGCCAAAACTGTACCAAGTACTCACTTACCCTCTACATTGAGTGAGCGCTGGATCTGGGCTAAATTGAAACAACTCATTGTCCAAACCACCAAGTTATTAGACAAGTACCAGTTAGCTTTGGCCAACCAAGCGTTAGTCAACTTTATTTGA", "aa": "MPTDLLVTGYDILFFWVLRMTFNSYFQTKQLPFKQVLIHGLVRDAQNRKMSKSLNNGINPMDLIRDYGADATRLFLTSNHTPGDDLIFNEQKLKSAANFLNKLWNVTKYVLQLGEQAKTVPSTHLPSTLSERWIWAKLKQLIVQTTKLLDKYQLALANQALVNFI", "aa_hexdigest": "0247efda14e376f468612d986773bbb0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 578445, "stop": 579242, "observations": { "mutation": [ 578747 ], "stop": [ 578747 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFE51D6", "query_cov": 1.0, "subject_cov": 0.44235924932975873, "identity": 1.0, "score": 331.0, "evalue": 5.92e-112, "gene_start": 1, "gene_end": 165, "reference_start": 1, "reference_end": 165, "reference_sequence": "MPTDLLVTGYDILFFWVLRMTFNSYFQTKQLPFKQVLIHGLVRDAQNRKMSKSLNNGINPMDLIRDYGADATRLFLTSNHTPGDDLIFNEQKLKSAANFLNKLWNVTKYVLQLGEQAKTVPSTHLPSTLSERWIWAKLKQLIVQTTKLLDKYQLALANQALVNFIWNDFCNTFIETIKQEDTALLPQLYTTAKTVLSTAVVMLSTVTPFLAERIYQQFHSGSVMQASWPTAKAVKPPKLFADVVEAVSSLRHYKANNQLVANQNLAVVLSGKAAPVVQNYFHFNWVDLRIEVNKTPGFQIKIVDNAANNLAHLEKQRSFYLAEVQRSQAITTNPAFLKKAPPHKVKAELLKLEEYQKKLAEVNHLIAKLTKAE" }, "paralog": false, "identity": 0.9924528301886792, "score": 523.0, "evalue": 5.85e-190, "description": "Internal stop codon at 578747. Nonsense mutation around 578747.", "psc": { "uniref90_id": "UniRef90_UPI001EFE51D6", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFE51D6", "UniRef:UniRef50_UPI001EFE51D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6" ], "product": "class I tRNA ligase family protein" } }, "id": "CNOCLNGIAL_1029", "locus": "GCA000143945_01025" }, { "type": "cds", "sequence": "contig_1", "start": 579393, "stop": 580151, "strand": "-", "frame": 1, "gene": "valS", "product": "valine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.9", "GO:0000166", "GO:0004832", "GO:0005524", "GO:0006438", "SO:0001217", "UniRef:UniRef50_UPI001EFEBE54", "UniRef:UniRef90_UPI001EFEBE54" ], "nt": "TTGTTTGAACAGGGCTTTATCTACCAAGCTGAAACCTTGGTTAACTGGGACACCAAGCTAAACACTGCCATCTCGAACATTGAAGTCATTAATAAACCAGTTGACCAACAGCTGTATTACATTGCTTATAAGTTAGCTAATAATCCCAAAAAACGTTTAGTGGTGGCAACTACTAGACCAGAAACGATCTTTGTTGATGTGTGTTTGTTTGTCCATCCCAAAGACAAGCACTACCACAGCTTTGTCAAACAAAAGGTAGTTAATCCGCTCACTGGTGCGTTAATGCCAGTGTTCACCGATAGTTATGTCGACAAGAAGTTTGGCACCGGTGTGTTGAAGTGCACCCCCGCCCATGACTTTAACGACTTTGCCTTAAACGAAAAGTACCGTTTACCGTTTGTAAGTTGCATTGACCACAATGGTTTGTTAAATGAGCACGCTAAGCAATTTACTGGTTTAACCGTAAGTGCAGCACGTCAGCAGGTAGTCGAGTTTTTACAAACGCAAAAACTACTAGTGAAAACTATGCCACTAACCAGTAACGTTGGTTTTTCCGAGCGCAGTGACACGGTCGTAGAACCGTTATTATCCAAGCAGTGGTTTGTCGATTTACCGAAACTCAAAAAAGCACTCGCCATTAAGAAATACCCCGAGTTAATTCCCAAGCGTTTTAACAAACAGGTAACAAGGTGGTTGAGTCAGCTCAAACCGTGGTGCATATCGCGCCAATTAATTTGGGGTCACCCTATTCCGGTGTGA", "aa": "MFEQGFIYQAETLVNWDTKLNTAISNIEVINKPVDQQLYYIAYKLANNPKKRLVVATTRPETIFVDVCLFVHPKDKHYHSFVKQKVVNPLTGALMPVFTDSYVDKKFGTGVLKCTPAHDFNDFALNEKYRLPFVSCIDHNGLLNEHAKQFTGLTVSAARQQVVEFLQTQKLLVKTMPLTSNVGFSERSDTVVEPLLSKQWFVDLPKLKKALAIKKYPELIPKRFNKQVTRWLSQLKPWCISRQLIWGHPIPV", "aa_hexdigest": "9f1bbed346d36710fa071d13504163d2", "start_type": "TTG", "rbs_motif": null, "genes": [ "valS" ], "truncated": "both", "pseudogene": { "start": 579175, "stop": 580451, "observations": { "deletion": [ 579320 ], "mutation": [ 579395, 580217 ], "stop": [ 579212, 579233, 579395, 580217 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFEBE54", "query_cov": 1.0, "subject_cov": 0.5163934426229508, "identity": 0.996, "score": 517.0, "evalue": 5.77e-182, "gene_start": 1, "gene_end": 252, "reference_start": 163, "reference_end": 414, "reference_sequence": "MDKQFSFQGQYDFKTVSTGLYDSWSSASFFKPQKNKVPFTAILPPPNLTGTLHIGHAFEVSITDQIMRFKRLRGYGVNWIPGFDHAGIATQTKYEKLARETNPEYFQAPRKQKVKMIMDWALTQGDTIQSQIKSLGASLNWNQVNFTLSKKASQIVNDSFIQLFEQGFIYQAETLVNWDTKLNTAISNIEVINKPVDQQLYYIAYKLANNPKKRLVVATTRPETIFVDVCLFVHPKDKHYHSFVKQKVVNPLTGALMPVFTDSYVDKKFGTGVLKCTPAHDFNDFALNEKYRLPFVSCIDHNGLLNEHAKQFTGLTVSAARQQVVEFLQTQKLLVKTMPLTSNVGFSERSDTVVEPLLSKQWFVDLPKLKKALAIKKYPELIPKRFNKQVTRWLSQLKPWCISRQLIWGHPIPVWTHKQSGALHVGSTAPTDKQNYTQSXHRCTRYLIFLITVTVNLFRLTQKQALCANWSFGNRVWHPLLLGLTDDL" }, "paralog": false, "identity": 0.9859154929577465, "score": 839.0, "evalue": 1.8e-311, "description": "Internal stop codon at 579212, 579233, 579395, 580217. Frameshift due to deletion around 579320. Nonsense mutation around 579395, 580217.", "psc": { "uniref90_id": "UniRef90_UPI001EFEBE54", "gene": "valS", "product": "valine--tRNA ligase", "ec_ids": [ "6.1.1.9" ], "uniref50_id": "UniRef50_UPI001EFEBE54", "go_ids": [ "GO:0000166", "GO:0004832", "GO:0005524", "GO:0006438" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFEBE54", "EC:6.1.1.9", "UniRef:UniRef50_UPI001EFEBE54", "GO:0000166", "GO:0004832", "GO:0005524", "GO:0006438" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFEBE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFEBE54" ], "product": "valine--tRNA ligase" } }, "id": "CNOCLNGIAL_1030", "locus": "GCA000143945_01026" }, { "type": "cds", "sequence": "contig_1", "start": 580215, "stop": 580460, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGAGTATCACTGACCAAATTATGCGCTTTAAGCGACTGCGGGGTTATGGCGTGAACTGGATTCCCGGATTTGACCATGCGGGGATTGCTACGCAAACCAAATACGAAAAGTTAGCACGGGAAACTAATCCCGAGTACTTCCAAGCGCCGCGTAAACAAAAGGTCAAAATGATTATGGATTGGGCCTTAACACAAGGGGACACCATTCAAAGTCAGCTCAAAAGTTTAGGGGCATCCTTAAACTGA", "aa": "MSITDQIMRFKRLRGYGVNWIPGFDHAGIATQTKYEKLARETNPEYFQAPRKQKVKMIMDWALTQGDTIQSQLKSLGASLN", "aa_hexdigest": "833acd389589ce28da4f8483f2d2daf6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 80, "aa_cov": 0.9753086419753086, "hmm_cov": 1.0, "evalue": 1.1078298716584231e-14, "score": 49.437538146972656, "start": 2, "stop": 80 } ], "seq_stats": { "molecular_weight": 9319.644399999996, "isoelectric_point": 9.81829319000244 }, "id": "CNOCLNGIAL_1031", "locus": "GCA000143945_01027" }, { "type": "cds", "sequence": "contig_1", "start": 580621, "stop": 581202, "strand": "-", "frame": 3, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872", "RefSeq:WP_010874837.1", "SO:0001217", "UniParc:UPI000003226E", "UniRef:UniRef100_P75303", "UniRef:UniRef50_P47577", "UniRef:UniRef90_P75303" ], "nt": "ATGGAGGCACGTTTTTTAAAAAGTGCAAGTGACTTAGAGAGTTGTCCCCAAGACAGTGTTAAGGAAGTCTGTTTTATGGGGCGGAGTAATGTCGGCAAATCAAGCCTCATTAATGCCTTTTTTCAAAAAAAGCTAGCTAAAACTTCCGCGACGCCGGGACGTACACAACTGTTAAACTTCTTTGAATACAACCGTAAACGCTTTGTCGATTTACCAGGTTATGGCTTTGCTAAACTGAGTAAAGTGCAAAAGGAAGCAATTACTAATTTATTAACGCAGTTCCTCAATTTTCGCCAAAACTTAACTGGTGTGGTACTAGTGATTGACAGCGGTGTAGTGACCGTTCAGGACCAGGAAGTGGTCAAAACCATTCTCCAAACGGGGTTAAATTTTTTGGTGATAGCCAATAAGTTTGATAAGCTCAACCAAAGCGAGCGCTTTCATACCCAGAATAAACTGGCACACTTTTTAAAAGTCAATCCTAATAAGTGTTTATTTGTTAGTGCCAAAACCGGGTATAACCTCCAAGTAATGCACAAACAAATCTTTGAGTTATTTAAAGCAGATGGACAAGCAATTTAG", "aa": "MEARFLKSASDLESCPQDSVKEVCFMGRSNVGKSSLINAFFQKKLAKTSATPGRTQLLNFFEYNRKRFVDLPGYGFAKLSKVQKEAITNLLTQFLNFRQNLTGVVLVIDSGVVTVQDQEVVKTILQTGLNFLVIANKFDKLNQSERFHTQNKLAHFLKVNPNKCLFVSAKTGYNLQVMHKQIFELFKADGQAI", "aa_hexdigest": "abd56841e4c3a5c5f3ceb4780214e371", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000003226E", "ncbi_nrp_id": "WP_010874837.1", "uniref100_id": "UniRef100_P75303", "db_xrefs": [ "SO:0001217", "UniParc:UPI000003226E", "RefSeq:WP_010874837.1", "UniRef:UniRef100_P75303" ] }, "ips": { "uniref100_id": "UniRef100_P75303", "uniref90_id": "UniRef90_P75303" }, "psc": { "uniref90_id": "UniRef90_P75303", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_P47577", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47577" ], "product": "Probable GTP-binding protein EngB" }, "genes": [ "yihA" ], "id": "CNOCLNGIAL_1032", "locus": "GCA000143945_01028" }, { "type": "cds", "sequence": "contig_1", "start": 581210, "stop": 581431, "strand": "-", "frame": 2, "gene": "yneF", "product": "UPF0154 MG335-1-like protein", "db_xrefs": [ "COG:COG3763", "COG:S", "GO:0016020", "KEGG:K09976", "RefSeq:WP_014574997.1", "SO:0001217", "UniParc:UPI00001833B6", "UniRef:UniRef100_Q9EXD7", "UniRef:UniRef50_Q9EXD7", "UniRef:UniRef90_Q9EXD7" ], "nt": "ATGAATGATCTTGCGCTTGCCTTAGGTTTGGGAATTCCCCTTTCCCTGTTAGTCGGTGTCATTATTGGCTATTTTATTTCTATCAAAATCTTTAAAAAACAGATTCGTGATAACCCACCAATCACCGAAAACCAAATTAAGGCGATGTATGCCAAAATGGGTCGTAAGCTTTCTGAAACACAAGTCAAGGAAATTATGCGCTCCATTAAAAACCAGAAGTAA", "aa": "MNDLALALGLGIPLSLLVGVIIGYFISIKIFKKQIRDNPPITENQIKAMYAKMGRKLSETQVKEIMRSIKNQK", "aa_hexdigest": "6b8518efc9d5c23459413584c515b25c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001833B6", "ncbi_nrp_id": "WP_014574997.1", "uniref100_id": "UniRef100_Q9EXD7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001833B6", "RefSeq:WP_014574997.1", "UniRef:UniRef100_Q9EXD7" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD7", "uniref90_id": "UniRef90_Q9EXD7" }, "psc": { "uniref90_id": "UniRef90_Q9EXD7", "gene": "YneF", "product": "UPF0154 protein MG335.1 homolog", "uniref50_id": "UniRef50_Q9EXD7", "cog_id": "COG3763", "cog_category": "S", "kegg_orthology_id": "K09976", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD7" ], "product": "UPF0154 protein MG335.1 homolog" }, "genes": [ "yneF" ], "id": "CNOCLNGIAL_1033", "locus": "GCA000143945_01029" }, { "type": "cds", "sequence": "contig_1", "start": 581490, "stop": 582119, "strand": "+", "frame": 3, "gene": "wcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.1.-", "GO:0005886", "GO:0006071", "GO:0046467", "GO:0046509", "KEGG:K19004", "SO:0001217", "UniRef:UniRef50_Q9ZB73", "UniRef:UniRef90_P75302" ], "nt": "ATGAACAAGCTAATTAGCATTCTGGTTCCTTGTTACCAATCCCAGCCCTTTCTCGATCGCTTTTTCAAATCACTTTTAAAGCAAGACTGGAACGGTGTTAAAGTAATCTTCTTTAATGATAACAAGCCAGATCCAACATACGAGATCCTAAAACAATTTCAACAAGCACATCCACAACTAGCGATTGAAGTACATTGTGGTGAGAAAAATGTCGGTGTAGGCGGTTCACGTGATCAACTAATAAATTATGTAGACACACCCTACTTTTACTTTGTTGATCCTGATGATGAATTTAGTGATCCCAACTGTTTTAAGGCAATAGTGGAAACAATTCAAGGAGAAAACTTTGATATTGCAGTGTTAAACAGTATTGTTTACCTCCAAATGTTGAAGAATGACTTTTTAATTAAACACATTCCGCTCAAAAACATCTTTCAGGGCAAGGTAAAACTCAATCCTGATAACACCGTAAATCATTTGCACTATATCCAGAATAACGACCAGTACATTTGGAACATTGTCATCAACACCGCCTTTTTCAAAGCATTGGATTTGCAGTTTGTTAACCGCTTTATTGAAGACATTGCGGTGTGGTTTCCGATTATGTTTAAAGCCCAAAAAGTGCTGTGA", "aa": "MNKLISILVPCYQSQPFLDRFFKSLLKQDWNGVKVIFFNDNKPDPTYEILKQFQQAHPQLAIEVHCGEKNVGVGGSRDQLINYVDTPYFYFVDPDDEFSDPNCFKAIVETIQGENFDIAVLNSIVYLQMLKNDFLIKHIPLKNIFQGKVKLNPDNTVNHLHYIQNNDQYIWNIVINTAFFKALDLQFVNRFIEDIAVWFPIMFKAQKVL", "aa_hexdigest": "116bcb9caa314d6839faa26b1fee38b6", "start_type": "ATG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 581490, "stop": 582422, "observations": { "mutation": [ 582117, 582294 ], "stop": [ 582117, 582294 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75302", "query_cov": 1.0, "subject_cov": 0.6129032258064516, "identity": 1.0, "score": 436.0, "evalue": 7.44e-153, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MNKLISILVPCYQSQPFLDRFFKSLLKQDWNGVKVIFFNDNKPDPTYEILKQFQQAHPQLAIEVHCGEKNVGVGGSRDQLINYVDTPYFYFVDPDDEFSDPNCFKAIVETIQGENFDIAVLNSIVYLQMLKNDFLIKHIPLKNIFQGKVKLNPDNTVNHLHYIQNNDQYIWNIVINTAFFKALDLQFVNRFIEDIAVWFPIMFKAQKVLWIDVNGVNYYLRPNSASTQKNSIKLLSFIEAYERLYFHLKKVGKLADFIDPNNKIESRFWRRQAFIWFSFINVSWMKAEFEQTKSVLQKLFDFMEANGIYDRVFTNKHHGIYLLWVNRLKHFKKLVQAQPHL" }, "paralog": false, "identity": 0.9935483870967742, "score": 631.0, "evalue": 3.41e-232, "description": "Internal stop codon at 582117, 582294. Nonsense mutation around 582117, 582294.", "psc": { "uniref90_id": "UniRef90_P75302", "gene": "WcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "ec_ids": [ "2.4.1.-" ], "uniref50_id": "UniRef50_Q9ZB73", "cog_id": "COG0463", "cog_category": "M", "kegg_orthology_id": "K19004", "go_ids": [ "GO:0005886", "GO:0046509", "GO:0006071", "GO:0046467" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75302", "EC:2.4.1.-", "UniRef:UniRef50_Q9ZB73", "COG:COG0463", "COG:M", "KEGG:K19004", "GO:0005886", "GO:0046509", "GO:0006071", "GO:0046467" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB73" ], "product": "Processive diacylglycerol beta-glycosyltransferase" } }, "id": "CNOCLNGIAL_1034", "locus": "GCA000143945_01030" }, { "type": "cds", "sequence": "contig_1", "start": 582147, "stop": 582296, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGACCGAACAGTGCTTCCACCCAGAAAAACAGCATTAAATTACTCTCCTTTATTGAGGCTTACGAACGGTTGTATTTCCACTTAAAGAAGGTGGGTAAGCTAGCTGATTTTATTGACCCTAACAATAAGATTGAAAGTCGTTTTTGA", "aa": "MRPNSASTQKNSIKLLSFIEAYERLYFHLKKVGKLADFIDPNNKIESRF", "aa_hexdigest": "9997683b229596b41da09e2cef7b4904", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5759.5947, "isoelectric_point": 9.77445468902588 }, "id": "CNOCLNGIAL_1035", "locus": "GCA000143945_01031" }, { "type": "cds", "sequence": "contig_1", "start": 582342, "stop": 582461, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCGGAATTTGAACAGACCAAAAGTGTGTTGCAAAAACTGTTTGACTTCATGGAAGCCAACGGCATCTACGACAGGGTATTTACTAATAAGCACCACGGTATTTACTTATTGTGA", "aa": "MKAEFEQTKSVLQKLFDFMEANGIYDRVFTNKHHGIYLL", "aa_hexdigest": "a6aa4d2c1b90fa3fa9ade9edace266a8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4663.333899999999, "isoelectric_point": 6.704352378845214 }, "id": "CNOCLNGIAL_1036", "locus": "GCA000143945_01032" }, { "type": "cds", "sequence": "contig_1", "start": 582526, "stop": 582822, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532763.1", "SO:0001217", "UniParc:UPI0002F4AA32", "UniRef:UniRef100_UPI0002F4AA32", "UniRef:UniRef50_A0A0H3DKQ0", "UniRef:UniRef90_A0A0H3DKQ0" ], "nt": "TTGGCAACCGAGTTGCAAGTGGTAGCTATTTTTTCAATCTCCAAAAAACAAATATTAATTATGAAAAATAAAAAAATTATCAAACTACCTTTTATTGATCACGCAATGAATTCACTCAATCAAAGTGAGCTAATTTCATTTGTAGCTTTTAACGAAGCCAATGAAGATAATAAACGCACACTTTACATAAAGATTCACGCTAATCAAAAGGAAATGGTTGTACAAGCCCAAGATGGAAAACAAACTCTAGAAAGAGAACAACTTTTTAAAAAGCTACTCGAAGCTTTAGCTTGTTAG", "aa": "MATELQVVAIFSISKKQILIMKNKKIIKLPFIDHAMNSLNQSELISFVAFNEANEDNKRTLYIKIHANQKEMVVQAQDGKQTLEREQLFKKLLEALAC", "aa_hexdigest": "9836dd356fafd039539fa67574de5c51", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002F4AA32", "ncbi_nrp_id": "WP_017532763.1", "uniref100_id": "UniRef100_UPI0002F4AA32", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F4AA32", "RefSeq:WP_017532763.1", "UniRef:UniRef100_UPI0002F4AA32" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002F4AA32", "uniref90_id": "UniRef90_A0A0H3DKQ0" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DKQ0", "uniref50_id": "UniRef50_A0A0H3DKQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DKQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DKQ0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11276.158399999993, "isoelectric_point": 9.098954963684083 }, "id": "CNOCLNGIAL_1037", "locus": "GCA000143945_01033" }, { "type": "cds", "sequence": "contig_1", "start": 583020, "stop": 583331, "strand": "-", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_484", "db_xrefs": [ "RefSeq:WP_010874840.1", "SO:0001217", "UniParc:UPI000013AB4F", "UniRef:UniRef100_P75301", "UniRef:UniRef50_P75076", "UniRef:UniRef90_P75301" ], "nt": "ATGGGCTTTTATGGCAATTTAAACCATATGGAAAAACGAAAAAGTGGTTACGTTACCCAAAAGCAGTTTAATGATTTCAAAAATTCCAACAATCAGCGGCTGATAAAAATTGAGAACACTTTGGTTTCTCAAGGTGAACAAATTAGTCAGCTAATCAAAGTTTCAATTCTTCAGGGTGAACAAATAAATAAGCTCACTGAAACTGTTGAAAAGCAGGGCGAACAAATTCAAACCCAAGGGGAAACGCTTAAGTTAATTCTTGAAACTCTTCAAGTAATTAACAAACGTTTGGATCGTTTGGAGTCTAAGTAG", "aa": "MGFYGNLNHMEKRKSGYVTQKQFNDFKNSNNQRLIKIENTLVSQGEQISQLIKVSILQGEQINKLTETVEKQGEQIQTQGETLKLILETLQVINKRLDRLESK", "aa_hexdigest": "9af49134a83744b70dcf9c7cd697c5d8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AB4F", "ncbi_nrp_id": "WP_010874840.1", "uniref100_id": "UniRef100_P75301", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AB4F", "RefSeq:WP_010874840.1", "UniRef:UniRef100_P75301" ] }, "ips": { "uniref100_id": "UniRef100_P75301", "uniref90_id": "UniRef90_P75301" }, "psc": { "uniref90_id": "UniRef90_P75301", "product": "UPF0134 protein MPN_484", "uniref50_id": "UniRef50_P75076" }, "pscc": { "uniref50_id": "UniRef50_P75076", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75076" ], "product": "UPF0134 protein MPN_038" }, "genes": [], "id": "CNOCLNGIAL_1038", "locus": "GCA000143945_01034" }, { "type": "cds", "sequence": "contig_1", "start": 583748, "stop": 584044, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTCGGGTCAGGGGACCCAAGTTAAGGGCAGTGTGCAGGGGAGTAGCCGAGCAGCTTCTGTTAGCGTTCAAACTACCCAGCAAAGCAGACAGCAATCGACTGATACCCAAGAATCGGAAGTGGTTAAGTTAGCCAAAGCGCTGTTGAAATCAAGTGCGGATTTGGCCAAACCCTTCACTGATAACCCCACCTTTAAAAAGGCCTTAACCGACATCCAATCCGAGTACAAGGATTATTTGGCTGCTGCGGGGAAGTTAAGTGAGTTTAAGAAAGATTTGGGGGAGGTGAGTTAA", "aa": "MGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKALLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAGKLSEFKKDLGEVS", "aa_hexdigest": "7acd74ec4be84e3f0a1153eba477fb91", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10415.452099999991, "isoelectric_point": 8.925663948059086 }, "id": "CNOCLNGIAL_1039", "locus": "GCA000143945_01035" }, { "type": "cds", "sequence": "contig_1", "start": 584093, "stop": 584860, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A8A20B3", "UniRef:UniRef90_UPI000A8A20B3" ], "nt": "GTGACCAAGTCCAACCTGCGCGGGGCCTTTAAGGGCGTGGGGTTAGCTACGGCCATTATTGACCAGTACGAGTACCTGGTGGGTGGGAGTAAGACGAGTTCGTTGCCCGAGGTCAAGGTAGATTCGAATAAGTCCAACCAAAATCCGCTGGATAGTTTTTTTATGGAGGGGAAGGATGCCGTGGCGATTAGATCCATAGTTTCGCGTGCGAAAATTGCCATGACCGACCAAACCCCCGGGTTTAAGGTCGACCCGGCGTTTGTCAAGGTGAAGCAGAATAATCAAACCGATGCGTTTTACACAACCCAGCGCAAACTGAGCGGCGGGCAAACTAATGGGGGTAGTAATAATTCCAATGATAAACACCACTACCTCCAAGACGCGGTGCGGTTGACGAGTAGTCAAGCAATGGCGGCAGCTTCCACTGGGGCAGATTCTAGTAGTGGTACCAATGTTGGTGGTAGCTCCGGGGGCAACTCTGTTTTAATTCCGCTCCCAAGGAGTGCCGCCCTTACCCACACCCAGCAACAAGTTCAACAAACCACCTCCACACTCCAAACCCCTGTTTACGCCCGTGGTGACGATGGTACATACGCCCTGGCAATTGACGGTGGTGATTATTTTTTGGCGAACAACAAACGCGATTTCACCAAACAAGCCGACATCTTGCTGTACCGCTATTTACAAGCTAAGAGTAATAACTTTAAGGAAAACGGCGTCGAGTTTAGTTTGAACTTACTGGAATCTGGCAGCCTGTTCCAAACGTGA", "aa": "MTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVDPAFVKVKQNNQTDAFYTTQRKLSGGQTNGGSNNSNDKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQT", "aa_hexdigest": "5c7a14f2b9f6e46b102105e77875d2cf", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 583790, "stop": 584974, "observations": { "mutation": [ 584095, 584893 ], "stop": [ 584095, 584893 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A8A20B3", "query_cov": 1.0, "subject_cov": 0.625, "identity": 0.996, "score": 403.0, "evalue": 4.03e-138, "gene_start": 1, "gene_end": 255, "reference_start": 39, "reference_end": 293, "reference_sequence": "MFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVDPAFVKVKQNNQTDAFYTTQRKLSGGQTNGGSNNSNDKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQTWAQTGLTAKLYGALVAMMGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKALLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAGKLSEFKKDLGEVS" }, "paralog": false, "identity": 0.9898477157360406, "score": 620.0, "evalue": 3.11e-226, "description": "Internal stop codon at 584095, 584893. Nonsense mutation around 584095, 584893.", "psc": { "uniref90_id": "UniRef90_UPI000A8A20B3", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI000A8A20B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A8A20B3", "UniRef:UniRef50_UPI000A8A20B3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A8A20B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A8A20B3" ], "product": "DUF3713 domain-containing protein" } }, "id": "CNOCLNGIAL_1040", "locus": "GCA000143945_01036" }, { "type": "cds", "sequence": "contig_1", "start": 584891, "stop": 585193, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE", "UniRef:UniRef90_UPI0006B4FEFE" ], "nt": "TTGCTCTATCTGTTTCCCCTCACCCAAGCGTTCTTCGCGTACCAAGCACCCAAGGACGGGCTCGACAGCTTATACGACCAAGCCGCGATTGGTAGTGCCTTACAGTTGGTCTATGCCTTCCCCGCGTTTCGCGAGCCCAATAATGGACAAAGCCAAGGTAAAACCACCTTCGACCCCACCCCCAACAGTGCCCAAAACTTCGGGGATTTCATTAAGGCGGTGTTTCCGGAGCAGAAGAATGGTCAAACCCAACAATCCAATACATCCTCCCGCACCGGGTTGTTTGACTGGCAAACCAAGTGA", "aa": "MLYLFPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTK", "aa_hexdigest": "5f3381ebf7f1d74115b541bf5cae3c11", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 584662, "stop": 585193, "observations": { "insertion": [ 584704 ], "mutation": [ 584893 ], "stop": [ 584893 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "query_cov": 1.0, "subject_cov": 0.5681818181818182, "identity": 1.0, "score": 205.0, "evalue": 3.38e-66, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MLYLFPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFLWRGRMPWRLDP" }, "paralog": false, "identity": 0.9886363636363636, "score": 349.0, "evalue": 2.49e-125, "description": "Internal stop codon at 584893. Frameshift due to insertion around 584704. Nonsense mutation around 584893.", "psc": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0006B4FEFE", "UniRef:UniRef50_UPI0006B4FEFE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE" ], "product": "DUF3713 domain-containing protein" } }, "id": "CNOCLNGIAL_1041", "locus": "GCA000143945_01037" }, { "type": "cds", "sequence": "contig_1", "start": 585287, "stop": 585670, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGACCAAAACTAAGTTTAATCCGTACCAATTTCGCAAACAATTTAAGTGGTTTAAAAACAACCCCCAATGGGTTAACTTTGACAACGCTGCTACCTCAATTGCGCTAGACACCGTTAGTCAAGCTTGCAAGGAATACTATGAGCTTTTTAGTGTCAACCCCCACAATAAAACCCCTGATCTCAACAACCAGATTATTGCCATTATTGCTGAAACCCGTCAACTAGTAGCGGACTGGTTTAATGTCACTGCACCTGAAATTATCTTTACAAGTTCAGCCACGGAGTCCATTAATTTGTTTGCCCATGGCTTAAAGCCCTGGATTAAACCCGGTGATGAAATTGTCTTAAAGGGTGATGAACACTCGGCTAACGTTTTGCCCTGA", "aa": "MTKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWFNVTAPEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLP", "aa_hexdigest": "9885f69ee1cc4c5f4ea2b762be9c8623", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 104, "aa_cov": 0.8110236220472441, "hmm_cov": 1.0, "evalue": 1.6621734576222523e-31, "score": 104.9789810180664, "start": 25, "stop": 127 } ], "seq_stats": { "molecular_weight": 14529.281099999984, "isoelectric_point": 6.269079399108887 }, "id": "CNOCLNGIAL_1042", "locus": "GCA000143945_01038" }, { "type": "cds", "sequence": "contig_1", "start": 585671, "stop": 586513, "strand": "+", "frame": 2, "gene": "csd", "product": "putative cysteine desulfurase", "db_xrefs": [ "COG:COG0520", "COG:E", "EC:2.8.1.7", "GO:0031071", "SO:0001217", "UniRef:UniRef50_Q49420", "UniRef:UniRef90_P75298" ], "nt": "GTGGCACTAGCTAAACAAACAAAGGCCAGACTAGTATGGGTCGAAAAGCAAGCTAACCAATCCTTAGAAGACACTTTTAAGTCATTGATTAACCCGAAAACTAAAGTAGTAGCCATTACCGCTACTTCCAATTTGTTTGGCAATTCAATTGACTTTGCTCAAATTGCCGATTACCTCAAACAAGTCAATCCTAAAGCTTTTGTAGCCGTGGATGCAGTGCAAACGGTGCAGCACAAGCAAATTGACATTGCCAAAACCCAGATCGACTTCCTAGCCTTTTCTACTCATAAGTTTTACGGTCCCACTGGACTGGGGGTAGCTTACATTAAAAAAAGCTTACAACCCCAATTACAACCCCTCAAACTCGGGGGTGATATCTTTACCCAGATTGACCCGGACAATACAATTCACTTTAAAAGTACACCCCTAAAGTTTGAAGCCGGTACCCCCAACATTATGGCCATCTATGCCTTAAACAAGCTGTTGCGCTTCTTTAAACAAAAGTTTAATTTTGCCCAAATGATGGCTTATGGTCACCAACTTAAACAACAAGCTTACGAACTGTTAAACAGTAATCCGCAAATTGTGCTGGCCAACCACGACCAGGATGTCCCGATTTTTTCCTTTAAACACCGCCAATTGGCTACCATTGATCTAGCCACTTTCCTAAACATCAATAAGATCATGGTACGCCAGGGCTCCATTTGTGTGGGACGTTACCGCAATAAGGATTACTTTGTGAGAGTTTCGTTAATGCACTATAACACAGTGAAGGAGTTGCAGTATTTAGCTAAACTCCTAGCAACTGATACCAAGACAATTATTAAAAACGTAATTAAGTAA", "aa": "MALAKQTKARLVWVEKQANQSLEDTFKSLINPKTKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKSLQPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLNSNPQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTKTIIKNVIK", "aa_hexdigest": "bbba49cb3e5110c6dc2b63919d160bbf", "start_type": "GTG", "rbs_motif": null, "genes": [ "csd" ], "truncated": "5-prime", "pseudogene": { "start": 585371, "stop": 586513, "observations": { "mutation": [ 585668 ], "stop": [ 585668 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75298", "query_cov": 1.0, "subject_cov": 0.6862745098039216, "identity": 0.996, "score": 548.0, "evalue": 3.58e-195, "gene_start": 1, "gene_end": 280, "reference_start": 129, "reference_end": 408, "reference_sequence": "MTKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWFNVTALEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLPWVALAKQTKARLVWVEKQANQSLEDTFKSLINPKTKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKSLQPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLNSNPQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTKTIIKNVIK" }, "paralog": false, "identity": 0.9947368421052631, "score": 746.0, "evalue": 2.78e-275, "description": "Internal stop codon at 585668. Nonsense mutation around 585668.", "psc": { "uniref90_id": "UniRef90_P75298", "gene": "csd", "product": "Probable cysteine desulfurase", "ec_ids": [ "2.8.1.7" ], "uniref50_id": "UniRef50_Q49420", "cog_id": "COG0520", "cog_category": "E", "go_ids": [ "GO:0031071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75298", "EC:2.8.1.7", "UniRef:UniRef50_Q49420", "COG:COG0520", "COG:E", "GO:0031071" ] }, "pscc": { "uniref50_id": "UniRef50_Q49420", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49420" ], "product": "Probable cysteine desulfurase" } }, "id": "CNOCLNGIAL_1043", "locus": "GCA000143945_01039" }, { "type": "cds", "sequence": "contig_1", "start": 586516, "stop": 586938, "strand": "+", "frame": 1, "gene": "iscU", "product": "putative MG337-like protein", "db_xrefs": [ "COG:COG0822", "COG:O", "GO:0005506", "GO:0016226", "GO:0051536", "RefSeq:WP_010874844.1", "SO:0001217", "UniParc:UPI0000139648", "UniRef:UniRef100_P75297", "UniRef:UniRef50_P75297", "UniRef:UniRef90_P75297" ], "nt": "ATGGATCGCAAAATAAGGGAGCAAATTATTGCCATTTACAGCAACTTACAACACAAACAGGACTTAACTCAGTTTGCCCATTGTTTAACCACTAAGGCTGATGATAACTGTGAGGACTTCTTTAACTTAGGCTTACAGTTTCAAAACAACCAACTAACAGCACTTGGTTTTAACGGGGAGGGTTGTATTATTTCGACAATTGCTAGTGAACTGACTTTAAGTGCGTTGGAAGGCAAAACAATCAAAGAAGCCATTGTCTTGTTAGAACAGTTTATGGAAGCTGTACAAACTGGTAGCTTAAGCACGCCACTACCCCAAGCTTTACAACTGTTGTGGGACTTTCAGATTGACCAGAAGCGTTTAAACTGCTTACTCCTAACACCACAGAATTTGCTACAGTGGCTTAAAGATTTTTCGCACTAA", "aa": "MDRKIREQIIAIYSNLQHKQDLTQFAHCLTTKADDNCEDFFNLGLQFQNNQLTALGFNGEGCIISTIASELTLSALEGKTIKEAIVLLEQFMEAVQTGSLSTPLPQALQLLWDFQIDQKRLNCLLLTPQNLLQWLKDFSH", "aa_hexdigest": "3a5136abf5d565a383c1df3dff163993", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0000139648", "ncbi_nrp_id": "WP_010874844.1", "uniref100_id": "UniRef100_P75297", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139648", "RefSeq:WP_010874844.1", "UniRef:UniRef100_P75297" ] }, "ips": { "uniref100_id": "UniRef100_P75297", "uniref90_id": "UniRef90_P75297" }, "psc": { "uniref90_id": "UniRef90_P75297", "gene": "IscU", "product": "Uncharacterized protein MG337 homolog", "uniref50_id": "UniRef50_P75297", "cog_id": "COG0822", "cog_category": "O", "go_ids": [ "GO:0005506", "GO:0051536", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_P75297", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75297" ], "product": "Uncharacterized protein MG337 homolog" }, "genes": [ "iscU" ], "id": "CNOCLNGIAL_1044", "locus": "GCA000143945_01040" }, { "type": "cds", "sequence": "contig_1", "start": 586919, "stop": 587095, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005B22982", "UniRef:UniRef90_UPI0005B22982" ], "nt": "ATGGCTTTACTAGTAGAACAAGCCTTGGACGCTGGTACGCAAGAGTTAGCCTTTTTAGACTTAGTGCGCGCTAACCAGGTGCAAAACAAACGTAGTCACCAGCAAATCCTGGTGGGTGACAAACGCTTGTATGATGCGTTAGGGCAAACGCTAGCGCTTAGTGCGAAAAATCTTTAA", "aa": "MALLVEQALDAGTQELAFLDLVRANQVQNKRSHQQILVGDKRLYDALGQTLALSAKNL", "aa_hexdigest": "d3438a7ea1725d724407af1ebccd8493", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 586919, "stop": 587197, "observations": { "mutation": [ 587143 ], "stop": [ 587143 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005B22982", "query_cov": 1.0, "subject_cov": 0.6304347826086957, "identity": 1.0, "score": 109.0, "evalue": 3.72e-30, "gene_start": 1, "gene_end": 58, "reference_start": 35, "reference_end": 92, "reference_sequence": "MSYVIQITNEDVNKISASWKTDKNSALGLKKEIFMALLVEQALDAGTQELAFLDLVRANQVQNKRSHQQILVGDKRLYDALGQTLALSAKNL" }, "paralog": false, "identity": 0.9782608695652174, "score": 171.0, "evalue": 9.94e-57, "description": "Internal stop codon at 587143. Nonsense mutation around 587143.", "psc": { "uniref90_id": "UniRef90_UPI0005B22982", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI0005B22982", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005B22982", "UniRef:UniRef50_UPI0005B22982" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005B22982", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005B22982" ], "product": "DUF3713 domain-containing protein" } }, "id": "CNOCLNGIAL_1045", "locus": "GCA000143945_01041" }, { "type": "cds", "sequence": "contig_1", "start": 587141, "stop": 587629, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCGACTGCTAGTGCCGGACTCACGGAACAAATCTTTAACAACTTTGGTCAGTTAGTAACAAGTAGTGACAAATCCGGGGCCTTGAGCCAGTACAAGGATAAAGCAACCCTGAAACGCTTAATCCAAAACACTAATTCCGATGCCGAGCTCAATGCCTTTGGTGAAGTGTTGCACCGTGCAGTTAATGTCGATACCAGTAACTTAGGACGTTTTAACTCCAGTGGGGAACCGTTGATTAGCTTTGACAACAAGAAGAAGTTCTTGGTTGATGTAGTGGACAAACTGGATGATGGTTACTTTAACAAGTTCGAAGGTTATGTTGGCCAAACCAAGGTGAAGATGTCTGATTCAAGTAGTAGTTCCCAAGGTAACGAAGACTATAAGAAAGCCAAAGCCGTACCACTCACCAAGGACTCGAGTGTCAAGACTTTGAGCTATGTCATTCAGATTACCAACGAGGACGTTAACAAAATTTCTGCTAGTTGA", "aa": "MSTASAGLTEQIFNNFGQLVTSSDKSGALSQYKDKATLKRLIQNTNSDAELNAFGEVLHRAVNVDTSNLGRFNSSGEPLISFDNKKKFLVDVVDKLDDGYFNKFEGYVGQTKVKMSDSSSSSQGNEDYKKAKAVPLTKDSSVKTLSYVIQITNEDVNKISAS", "aa_hexdigest": "ddb6b5ca80046837f5ec6f411d81cd2d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17679.421000000002, "isoelectric_point": 6.587207221984863 }, "id": "CNOCLNGIAL_1046", "locus": "GCA000143945_01042" }, { "type": "cds", "sequence": "contig_1", "start": 587810, "stop": 587965, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTATGATGATACGGCTACGGCATCAGCAGCCGCAGCAAAAGCGCAAGTAGCAGTATTAAAGACAGCTCAGGCAACAAATACTCAAAGCGACAACCCCTTCAATAAGTTTGTCCAAAACCCCGATTATGTCCAGGGTTCGGAAACCAACTGA", "aa": "MSYDDTATASAAAAKAQVAVLKTAQATNTQSDNPFNKFVQNPDYVQGSETN", "aa_hexdigest": "345d1816f56bb0a4f4457f4db144b964", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5381.7197000000015, "isoelectric_point": 4.359459877014161 }, "id": "CNOCLNGIAL_1047", "locus": "GCA000143945_01043" }, { "type": "cds", "sequence": "contig_1", "start": 588050, "stop": 588226, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCACTTTCAAGTAGCGCAGCAGTTGCAAATGCTGTAGCTTCAAGTGGCATGCAAAAATCTGGTGATCAAACCCTCTTGGAACTCGGTAAAAAAGCGTTTGAAAGTGAACTGGAAGCATCTAGTAGTGATGGGCAGTACAAGTACCTGCGCTTCTTGTCCACCTTAATGTGA", "aa": "MVSLSSSAAVANAVASSGMQKSGDQTLLELGKKAFESELEASSSDGQYKYLRFLSTLM", "aa_hexdigest": "b379422fe7e084317980c5e62fef166e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6121.832899999998, "isoelectric_point": 5.052213478088378 }, "id": "CNOCLNGIAL_1048", "locus": "GCA000143945_01044" }, { "type": "cds", "sequence": "contig_1", "start": 588572, "stop": 589219, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTCGGGTCAGGGGACCCAAGTTAAGGGCAGTGTGCAGGGGAGTAGCCGAGCAGCTTCTGTTAGCGTTCAAACTACCCAGCAAAGCAGACAGCAATCGACTGATACCCAAGAATCGGAAGTGGTTAAGTTAGCCAAGTCCCTGCTGAAATCAAGTGCGGATTTGGCCAAACCCTTCACTGATAACCCCACCTTTAAAAAGGCCTTAACCGACATCCAATCCGAGTACAAGGATTATTTGGCTGCTGCGGAGAAGTTAAGTGAGTTTAAGAAAGATTTGGGGGAGGTGAGTGGCTTACAGCAAGCTATTATTGACCGCGCTGATAAGTACATTCAACTAGAAAAACAAGCCCAAAAGAGTGCGATTGGCTTGGGCCAACCGCTCCCTTACCAACGCGCTAGTGATGGTAGTTACCCCGCTTTAGAGAAGTTCTTTATTCCGGAAGATAGTGCAGCAGATGGAAAAGTAAAAGCATCTGAATCAGGATCTGCCGCTTTAGTAACACTAAAGACAACAGATAGTCAAAAAAGCACAAACACCGTTAAACAACCGGACATCAAACCGACTCGGGAAAACAACGACAAAAAGTTAAAGCAGTTAACTAGTGATGTAGAAACTAAAGCATCTAGTTTAATCACCAAATGA", "aa": "MGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKSLLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAEKLSEFKKDLGEVSGLQQAIIDRADKYIQLEKQAQKSAIGLGQPLPYQRASDGSYPALEKFFIPEDSAADGKVKASESGSAALVTLKTTDSQKSTNTVKQPDIKPTRENNDKKLKQLTSDVETKASSLITK", "aa_hexdigest": "7094b0dce8567c22b73531966c1282a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23165.593100000002, "isoelectric_point": 9.030682945251463 }, "id": "CNOCLNGIAL_1049", "locus": "GCA000143945_01045" }, { "type": "cds", "sequence": "contig_1", "start": 589268, "stop": 590029, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_485", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75300", "UniRef:UniRef90_P75300" ], "nt": "GTGACCAAGTCCAACCTGCGCGGGGCCTTTAAGGGCGTGGGGTTAGCTACGGCCATTATTGACCAGTACGAGTACCTGGTGGGTGGGAAAAAAACGAGTTCGTTGCCCGAGGTCAAGGTAGATTCGAATAAGTCCAACCAAAATCCGCTGGATAGTTTTTTTATGGAGGGGAAGGATGCGGTGGCGATTAGATCCATAGTTTCGCGTGCGAAAATTGCCATGACCGACCAAACCCCCGGGTTTAAGGTCAACCCGGCGTTTGTCAAGGTGAAGCAGAGTCAGCAGAACGATACCTTTTACCAAAACCAGCGCAAACTGAGCGGCGGGCAAAGTGGGGATAATAACTCCCAGGGTAAACACCACTACCTCCAAGACGCGGTGCGGTTGACGAGTAGTCAAGCAATGGCGGCAGCTTCTACTGGGGCAGATTCTAGTAGTGGTACCAATGTTGGTGGTAGCTCCGGGGGCAACTCTGTTTTAATTCCGCTCCCAAGGAGTGCCGCCCTTACCCACACCCAGCAACAAGTTCAACAAACCACCTCCACACTCCAAACTCCTGTTTACGCCCGTGGTGACGATGGTACATACGCCCTGGCAATTGACGGTGGTGATTATTTTTTGGCGAACAACAAACGCGATTTCACCAAACAAGCCGACATCTTGCTGTACCGCTATTTACAAGCTAAGAGTAATAACTTTAAGGAAAACGGCGTCGAGTTTAGTTTGAACTTACTGGAATCTGGCAGCCTGTTCCAAACGTGA", "aa": "MTKSNLRGAFKGVGLATAIIDQYEYLVGGKKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVNPAFVKVKQSQQNDTFYQNQRKLSGGQSGDNNSQGKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQT", "aa_hexdigest": "0d8906a50234f53318bc3abe30f76133", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 588965, "stop": 590329, "observations": { "mutation": [ 589270, 590062 ], "stop": [ 589270, 590062 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75300", "query_cov": 1.0, "subject_cov": 0.49317738791423, "identity": 0.992, "score": 478.0, "evalue": 4.41e-166, "gene_start": 1, "gene_end": 253, "reference_start": 102, "reference_end": 354, "reference_sequence": "FFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVNPAFVKVKQSQQNDTFYQNQRKLSGGQSGDNNSQGKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQTWAQTGLTAKLYGALVAMMGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKSLLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAEKLSEFKKDLGEVSGLQQAIIDRADKYIQLEKQAQKSAIGLGQPLPYQRASDXVVTPL" }, "paralog": false, "identity": 0.9889867841409692, "score": 832.0, "evalue": 5.34e-308, "description": "Internal stop codon at 589270, 590062. Nonsense mutation around 589270, 590062.", "psc": { "uniref90_id": "UniRef90_P75300", "product": "Uncharacterized protein MPN_485", "uniref50_id": "UniRef50_P75300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75300", "UniRef:UniRef50_P75300" ] }, "pscc": { "uniref50_id": "UniRef50_P75300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75300" ], "product": "Uncharacterized protein MPN_485" } }, "id": "CNOCLNGIAL_1050", "locus": "GCA000143945_01046" }, { "type": "cds", "sequence": "contig_1", "start": 590060, "stop": 590365, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE", "UniRef:UniRef90_UPI0006B4FEFE" ], "nt": "GTGGAGGAAAACCCCCTCCCCCTCACCCAAGCCTTCTTCGCGTACCAAGCACCCAAGGACGGGCTCGACAGCTTATACGACCAAGCCGCGATTGGTAGTGCCTTACAGTTGGGCTATGCCTTCCCCGCGTTTCGCGAGCCCAATAATGGACAAAGCCAAGGTAAAACCACCTTCGACCCCACCCCCAACAGTGCCCAAAACTTCGGGGATTTCATTAAGGCGGTGTTTCCGGAGCAGAAGAATGGTCAAACCCAACAATCCAATACATCCTCCCGCACCGGGTTGTTTGACTGGCAAACCAAGTGA", "aa": "MEENPLPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLGYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTK", "aa_hexdigest": "ed2067ba64981a82eb1574b3db4a6a8e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 589831, "stop": 590347, "observations": { "insertion": [ 589873 ], "mutation": [ 590062 ], "stop": [ 590062 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "query_cov": 0.9405940594059405, "subject_cov": 0.5397727272727273, "identity": 0.9890000000000001, "score": 191.0, "evalue": 1.07e-60, "gene_start": 7, "gene_end": 101, "reference_start": 6, "reference_end": 100, "reference_sequence": "MLYLFPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFLWRGRMPWRLDP" }, "paralog": false, "identity": 0.9766081871345029, "score": 333.0, "evalue": 9e-119, "description": "Internal stop codon at 590062. Frameshift due to insertion around 589873. Nonsense mutation around 590062.", "psc": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0006B4FEFE", "UniRef:UniRef50_UPI0006B4FEFE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE" ], "product": "DUF3713 domain-containing protein" } }, "id": "CNOCLNGIAL_1051", "locus": "GCA000143945_01047" }, { "type": "cds", "sequence": "contig_1", "start": 590369, "stop": 590521, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTGACTGATGGTAGTGATCAGTTTAAACCCACTACCAAAGATAACCTCGACAGTACTAGCAACAAAATCAAGTTTGTTAATTCCAAACCAAATGATCCTAACGGGGAGTTCTTTGCTAACCTCCAGGCTTATCTATTTGCCCAGTGA", "aa": "MQLTDGSDQFKPTTKDNLDSTSNKIKFVNSKPNDPNGEFFANLQAYLFAQ", "aa_hexdigest": "55cc7661bf6274e0c4f2776d45f597bc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5627.124100000001, "isoelectric_point": 4.854868507385253 }, "id": "CNOCLNGIAL_1052", "locus": "GCA000143945_01048" }, { "type": "cds", "sequence": "contig_1", "start": 590648, "stop": 590956, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE6F233", "UniRef:UniRef90_UPI002FE6F233" ], "nt": "GTGGTGCTAGCGGCGTGTTCTGCACTCAATACCTCCAATCTGTTTCCCCGTCAAAACCGCTCCAAGCAGTTGATTGGGTTTACCGAAAACAACATCATTAAACCTGAAGCTGTACTAAAAGCCGCTTTAGCTGAGGACAATGGTACGGAAACCATTTTAAGGGTTAACTTTGGTGAAGCGTTAAAGAGTTGGTACCAAAACAATAAGGATCGCAACATTGCTACCCGTTTAACTATCTTTAGTGAAAACGTGGAGGATGAACATGATAACCTGTTGGACCAAAAACAACAGGCCGAACCGATTAATTGA", "aa": "MVLAACSALNTSNLFPRQNRSKQLIGFTENNIIKPEAVLKAALAEDNGTETILRVNFGEALKSWYQNNKDRNIATRLTIFSENVEDEHDNLLDQKQQAEPIN", "aa_hexdigest": "807c034373d4ba9a80798388f61d708a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 590345, "stop": 590953, "observations": { "mutation": [ 590371, 590596, 590650 ], "stop": [ 590371, 590596, 590650 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE6F233", "query_cov": 0.9901960784313726, "subject_cov": 0.4469026548672566, "identity": 0.99, "score": 197.0, "evalue": 4.02e-62, "gene_start": 2, "gene_end": 102, "reference_start": 1, "reference_end": 101, "reference_sequence": "MLAACSALNTSNLFPRQNRSKQLIGFTENNIIKPEAVLKAALAEDNGTETILRVNFGEALKSWYQNNKDRNIATRLTIFSENVEDEHDNLLDQKQQAEPINWPIELQKEYDQWGGSESSWKALKLYDRLIADFQSLIFSNVVANVQLTDGSDQFKPTTKDNLDSTSNKIKFVNSKPNDPNGEFFANLQAYLFAQWVVEENPLPLTQAFFAYQAPKDGLDSLYDQAA" }, "paralog": false, "identity": 0.9752475247524752, "score": 390.0, "evalue": 8.22e-141, "description": "Internal stop codon at 590371, 590596, 590650. Nonsense mutation around 590371, 590596, 590650.", "psc": { "uniref90_id": "UniRef90_UPI002FE6F233", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI002FE6F233", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE6F233", "UniRef:UniRef50_UPI002FE6F233" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE6F233", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE6F233" ], "product": "DUF3713 domain-containing protein" } }, "id": "CNOCLNGIAL_1053", "locus": "GCA000143945_01049" }, { "type": "cds", "sequence": "contig_1", "start": 591519, "stop": 592529, "strand": "-", "frame": 1, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "COG:COG0468", "COG:L", "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664", "KEGG:K03553", "RefSeq:WP_010874846.1", "SO:0001217", "UniParc:UPI00001334FE", "UniRef:UniRef100_P78014", "UniRef:UniRef50_P78014", "UniRef:UniRef90_P78014" ], "nt": "ATGGTACAAAAAGAAATGATTAATAAGAAAATTAGCCAAGACAACAGTTTTATTCAAAACAATAACTTGGCAGACTTTGATTTTTTAGATGCAAAGAAAAACAGTGAAATTAAAACTGTCTCAACTGGAAGCCTTCATTTAGATGAAGCGCTCGGCACTGGTGGTTTGCCACTAGGGCGCATTGTAGAACTTTATGGTAACGAGTCCTCGGGTAAAACCACGGTCGCGTTACATGCAGTCGCTAGCTTTCAAAAAGCGGGCAAAGTGGCCTGTTACATTGATGCTGAGGGTGCACTAGATTTAAGTTACGCCAAGGCAATTGGGATTGACTTGGGTAAATTACTAGTAGCCCATCCCAAGCATGGGGAAAACGCCTTTGCCCTAATGGAGTCACTGATTAAGACCAACAAAGTGGCCTTAATTGTGGTGGACTCCGTGGCGGCTTTGATTCCTAAGCAAGAACTGGAGGGCAACATGGATGACCAGACAATAGGACTCCATGCGCGGATGATGTCTAAGGGACTACGCCGTGTGCAGTCACTCTTACCTGAATCTGACACCTGCTTACTGTTTATTAACCAACTCCGGGAAAAACCCGGAGTCATGTTTGGTAATGGGGAAGTAACTACTGGGGGTCGGGCGTTGAAGTTCTACGCTTCGATGCGGATGGAGGCCAAACGCAGTGAACTGCTCAAAGACCGCTTTGGTAACTATGTCGGCATTAAGAGTAAGTTAACCGTGTCCAAGAATAAGGTAGCGCGCCCGTTTGGTGTTGCCTTTTTGGAAATTATGTTTAACCGCGGTATTGTGTATGAACACGAAGTGATTGAGTTAGCGCTTAAACACAATGTGGTGGTGCGCAGTGACAATGCATATTCGTTTAAGAGCCAAAACATTGCGATTGGCAAAGAGAAGCTCTTTAGCGTGTTGGCGGAAAAACCAGAGTTGTTTGAACAAATTAAGCAGCTCACGATTAAGCAAATCCACAGCCCGCCACCCCCAGCGAGCTAG", "aa": "MVQKEMINKKISQDNSFIQNNNLADFDFLDAKKNSEIKTVSTGSLHLDEALGTGGLPLGRIVELYGNESSGKTTVALHAVASFQKAGKVACYIDAEGALDLSYAKAIGIDLGKLLVAHPKHGENAFALMESLIKTNKVALIVVDSVAALIPKQELEGNMDDQTIGLHARMMSKGLRRVQSLLPESDTCLLFINQLREKPGVMFGNGEVTTGGRALKFYASMRMEAKRSELLKDRFGNYVGIKSKLTVSKNKVARPFGVAFLEIMFNRGIVYEHEVIELALKHNVVVRSDNAYSFKSQNIAIGKEKLFSVLAEKPELFEQIKQLTIKQIHSPPPPAS", "aa_hexdigest": "d278d8e2ad4383582cac2f1e1bd6f327", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001334FE", "ncbi_nrp_id": "WP_010874846.1", "uniref100_id": "UniRef100_P78014", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001334FE", "RefSeq:WP_010874846.1", "UniRef:UniRef100_P78014" ] }, "ips": { "uniref100_id": "UniRef100_P78014", "uniref90_id": "UniRef90_P78014" }, "psc": { "uniref90_id": "UniRef90_P78014", "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_P78014", "cog_id": "COG0468", "cog_category": "L", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664" ] }, "pscc": { "uniref50_id": "UniRef50_P78014", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78014" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "CNOCLNGIAL_1054", "locus": "GCA000143945_01050" }, { "type": "cds", "sequence": "contig_1", "start": 592603, "stop": 593493, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_491", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75295", "UniRef:UniRef90_P75295" ], "nt": "ATGAAATTAACACTTTGGCTGGTGTTGGGCGCTGTCGGAGTCGGTGCAGTTGGTACGGGTGTGGGCTTTGGTACCAAGTACTTTTTAGACCACAAAGCCAAAAGTAACGTGGACGTTGCGTTTAAACAACGTTCACTACGCAAACAGGTCAATGTGGGCTTTTGGAATGCACTCAACTTCTTGGGTAGTGAAAGAAGTTTCGGCAACCAAAACAGTAAGGTTAAAACCAAGGGGATTGCTGAAATTATTAACCTCCTTAAGTACGACATTGTCGGTTTAGCGGAAATTCAACCATCCACTGAAAGTACTGCTGAACGGTTTGTCGACCAACTCAACGAGTACGCTGATCCTTATACCTCCTGGTCTTATGATCTATCTCCCGTAACTACCAGTAAGACTGCTAGTGAAGGCCAGAAGGAACGGATCTTAATTGTTTACAACAGCCAGAGTGTCAAAATGGAGGGACAAGGTTGGTTTTATGACAATCCCGAAATGGACATTAGTAACTATGTCAGTGATGGGAGTTCTTCCAAAACAAGAACACCTAAGAAAACTAAAACTAGTAAAAAGAAGCCTACTAAAAAGAAAAGTTCAAGAAGCAAATCTTCCAAAGGTAGTAAGAAACAAAAAACTACCAATGAAAGTGAAAGTGAAACTTTAGAACTCAAACTGGAACAACGCCGTGTAACAACACGCTCACAATCAAAGCAACAAAAGGGGCAAGAACAAGCAACTGATCAAACTGATAGTGAGGGTGTGACTACTGAAGAAGGAGCAGATAACACTGACACTGAATTAGTTGAAACCACAGCGGAAACAACCGAACAAGAAGCTACCACGGAAAGTACAAAAGACACTAAGGAAACTAAAGATAGAATCAAAGTGGACTGA", "aa": "MKLTLWLVLGAVGVGAVGTGVGFGTKYFLDHKAKSNVDVAFKQRSLRKQVNVGFWNALNFLGSERSFGNQNSKVKTKGIAEIINLLKYDIVGLAEIQPSTESTAERFVDQLNEYADPYTSWSYDLSPVTTSKTASEGQKERILIVYNSQSVKMEGQGWFYDNPEMDISNYVSDGSSSKTRTPKKTKTSKKKPTKKKSSRSKSSKGSKKQKTTNESESETLELKLEQRRVTTRSQSKQQKGQEQATDQTDSEGVTTEEGADNTDTELVETTAETTEQEATTESTKDTKETKDRIKVD", "aa_hexdigest": "ec14b66b91a21cd741a88872ee11b0df", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 592603, "stop": 593796, "observations": { "mutation": [ 593491 ], "stop": [ 593491 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75295", "query_cov": 1.0, "subject_cov": 0.6244725738396625, "identity": 0.9590000000000001, "score": 469.0, "evalue": 1.45e-162, "gene_start": 1, "gene_end": 296, "reference_start": 1, "reference_end": 296, "reference_sequence": "MKLTLWLVLGAVGVGAVGTGVGFGTKYFLDHKAKSNVDVAFKQRSLRKQVNVGFWNALNFLGSERSFGNQNSKVKTKGIAEIINLLKYDIVGLAEIQPSTESTAERFVDQLNEYADPYTSWSYDLSPVTTSKTASEGQKERILIVYNSQSVKMEGQGWFYDNPEMDISNYVSDGSSSKTRTPKKTKTSKKKPIKKKSSKSKSSKGSKKQKTTNESESETLELKLEQRRVTTRSQSKQQKGQEQATDQTDSEGVTTEEGADNTDTELVETTAETTEQEATTKSTKDTKETKDRTKVDWSRPPYSAEFSTQKGKVVVAFGHFDSPGANTKRGEKIAKMGKGQGAHEYFEAQTTFKAMDSIKQHFNNENILFMADTNIKFGNQAAAFGESKDYTFLTEDNEAWKSSLGTKSGYANPYDKIITSNSFKNKVLDQAETYWNYFDRYLKTKGHNSYLFDLASWGTKPRTISDHAPVGVLF" }, "paralog": false, "identity": 0.9697732997481109, "score": 694.0, "evalue": 1.06e-253, "description": "Internal stop codon at 593491. Nonsense mutation around 593491.", "psc": { "uniref90_id": "UniRef90_P75295", "product": "Uncharacterized protein MPN_491", "uniref50_id": "UniRef50_P75295", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75295", "UniRef:UniRef50_P75295", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P75295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75295" ], "product": "Uncharacterized protein MPN_491" } }, "id": "CNOCLNGIAL_1055", "locus": "GCA000143945_01051" }, { "type": "cds", "sequence": "contig_1", "start": 593545, "stop": 593802, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTTTGGTCATTTTGACTCTCCGGGGGCAAACACTAAGCGGGGTGAAAAAATCGCTAAGATGGGCAAGGGTCAAGGTGCCCATGAATACTTTGAAGCGCAAACTACCTTCAAGGCAATGGATTCCATTAAGCAGCACTTTAACAACGAAAACATCCTGTTTATGGCTGACACTAACATTAAATTCGGTAATCAAGCCGCTGCTTTTGGTGAAAGCAAAGATTACACCTTTTTAACTGAAGATAATGAAGCGTGA", "aa": "MAFGHFDSPGANTKRGEKIAKMGKGQGAHEYFEAQTTFKAMDSIKQHFNNENILFMADTNIKFGNQAAAFGESKDYTFLTEDNEA", "aa_hexdigest": "d8169903d19ba3a30488b160954a6716", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9459.345299999994, "isoelectric_point": 5.492601966857909 }, "id": "CNOCLNGIAL_1056", "locus": "GCA000143945_01052" }, { "type": "cds", "sequence": "contig_1", "start": 594274, "stop": 594921, "strand": "-", "frame": 3, "gene": "ulaE", "product": "L-ribulose-5-phosphate 3-epimerase", "db_xrefs": [ "COG:COG3623", "COG:G", "EC:5.1.3.22", "GO:0005975", "GO:0016861", "GO:0019854", "GO:0034015", "SO:0001217", "UniRef:UniRef50_P75294", "UniRef:UniRef90_P75294" ], "nt": "TTGAGCATTGATGAATCGGATGCTAGGTTACAACGCTTGGATTGGAGTGATACCGAAATTAACCAACTCCACAATGAGTTACAAGCACAAACCTTTTGTTTAAACTCAATGTGTTTAAGTGCCCACCGGCGCTTTCCTTTGGGTTCCAAGAATAAAACTACTGTCCAACAGGGCTTAACCATTTTTGAAAAGGCCTGTGTGTTAGCACGCAAACTAGGGATTCGTATTATCCAGTTAGCGGCTTATGATGTGTATTATGAGCCACATGACACAGAAACAGAACGCAATTTCATTACTAACATGCGGAAAGTGGCAGAGTTAGCGCAAAAGTACGCTGTTACCATTGCCTTTGAGGTGATGGACACGCCGTTTGCTGGCACGATTGTGCGTTGTTTAAACTTGATTAAGCGGATTGGTAAGGCTAACATTTTGCTCTATCCGGATATTGGTAACCTATCGCAATTTAGTACGGCAGTCTTTGATGAAATCGCATTAGGTCAAGACAAGATAGTGGGCTTTCACTTTAAAGATACTTTACCCAAGCAGTTTAAGGAAGTACCGTTTGGCACAGGTACAGCCCAATTTGAAGCAGCTTTAAAAGCCATTCACCAGTATGTACCAACAGTACCAATTCTAATTGAAATGTGA", "aa": "MSIDESDARLQRLDWSDTEINQLHNELQAQTFCLNSMCLSAHRRFPLGSKNKTTVQQGLTIFEKACVLARKLGIRIIQLAAYDVYYEPHDTETERNFITNMRKVAELAQKYAVTIAFEVMDTPFAGTIVRCLNLIKRIGKANILLYPDIGNLSQFSTAVFDEIALGQDKIVGFHFKDTLPKQFKEVPFGTGTAQFEAALKAIHQYVPTVPILIEM", "aa_hexdigest": "5a012f6ffce7f5176551567a9fdeded2", "start_type": "TTG", "rbs_motif": null, "genes": [ "ulaE" ], "truncated": "both", "pseudogene": { "start": 594166, "stop": 595083, "observations": { "mutation": [ 594276, 594972 ], "stop": [ 594276, 594972 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75294", "query_cov": 1.0, "subject_cov": 0.7049180327868853, "identity": 0.995, "score": 427.0, "evalue": 4.19e-150, "gene_start": 1, "gene_end": 215, "reference_start": 55, "reference_end": 269, "reference_sequence": "MLVIHFKPYNNLKMSFTSTENKHLLGVYEKAINNKFAWKDKIAIAKQASFDFIELSIDESDARLQRLDWSDTEINQLHNELQAQTFCLNSMCLSAHRRFPLGSKNKTTVQQGLTIFEKACVLARKLGIRIIQLAAYDVYYEPHDTETERNFITNMRKVAELAQKYAVTIAFEVMDTPFAGTIVRCLNLIKRIGKANILLYPDIGNLSQFSTAVFDEIALGQDKIVGFHFKDTLPKQFKEVPFGTGTAQFEAALKAIHQYVPTVPILIEMWSKNDPAESTVQNVAQLKQAKQFYEQQWDLALKRVK" }, "paralog": false, "identity": 0.9934426229508196, "score": 599.0, "evalue": 3.47e-220, "description": "Internal stop codon at 594276, 594972. Nonsense mutation around 594276, 594972.", "psc": { "uniref90_id": "UniRef90_P75294", "gene": "ulaE", "product": "L-ribulose-5-phosphate 3-epimerase", "ec_ids": [ "5.1.3.22" ], "uniref50_id": "UniRef50_P75294", "cog_id": "COG3623", "cog_category": "G", "go_ids": [ "GO:0005975", "GO:0016861", "GO:0019854", "GO:0034015" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75294", "EC:5.1.3.22", "UniRef:UniRef50_P75294", "COG:COG3623", "COG:G", "GO:0005975", "GO:0016861", "GO:0019854", "GO:0034015" ] }, "pscc": { "uniref50_id": "UniRef50_P75294", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75294" ], "product": "Probable L-ribulose-5-phosphate 3-epimerase UlaE" } }, "id": "CNOCLNGIAL_1057", "locus": "GCA000143945_01053" }, { "type": "cds", "sequence": "contig_1", "start": 595025, "stop": 595204, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACGCTTAGCGGATTTGGGCTTTAAGGTCACCGTTACCGGCGGCATTACCCTCAACGACATCCAGTTATTTAAGGATATTCCCATTTATATCTTTATTGCTGGGCGTACCATTAGGGATGCTAGTGATCCACTTCAAACCGTACAACAATTTAAAGATGAGTTTCACAAGTACTGA", "aa": "MKRLADLGFKVTVTGGITLNDIQLFKDIPIYIFIAGRTIRDASDPLQTVQQFKDEFHKY", "aa_hexdigest": "cf70789c30d3148d68fda7796cf1e1f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6787.792700000002, "isoelectric_point": 8.085318565368652 }, "id": "CNOCLNGIAL_1058", "locus": "GCA000143945_01054" }, { "type": "cds", "sequence": "contig_1", "start": 595295, "stop": 595675, "strand": "-", "frame": 2, "gene": null, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "nt": "ATGGCTTTACCTTTAATCCAAATTGCGCTTGATAACCTCAGTTTAGCGAGTGCTTTAAATGACCTGGCTAAAGTCGGGGATGCTGTTGATGTTATTGAAGTGGGCACTATCTTACTAACAGCGGAGGGCGTTAATGCTGTTAAAGAAATTGCCAAACGCTATCCCCATAAGTTAATAGTGGCTGATGGCAAGATTGCTGATGCGGGGAAGGTGTTTAGCCAAATGTTCTTTGATGCTGGGGCACACTTTACCACGGTCATTTGTGCAGCGGAATTGCCCACCGTAAAGGATGTGGTAACTGTGGGTAACAGTTACACGCCCATAAAAGAAACCCAGGTGGAAATGACCAGTAACTTCACTTGGGAGCAGGTAACGCAGTGA", "aa": "MALPLIQIALDNLSLASALNDLAKVGDAVDVIEVGTILLTAEGVNAVKEIAKRYPHKLIVADGKIADAGKVFSQMFFDAGAHFTTVICAAELPTVKDVVTVGNSYTPIKETQVEMTSNFTWEQVTQ", "aa_hexdigest": "67d4e42584165d37aaba7faed86f9005", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B8401", "query_cov": 0.8095238095238095, "subject_cov": 0.9902912621359223, "identity": 0.5, "score": 107.0, "evalue": 6.18e-28, "valid": false, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "uniref50_id": "UniRef50_UPI00387B8401" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B8401", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein" }, "genes": [], "id": "CNOCLNGIAL_1059", "locus": "GCA000143945_01055" }, { "type": "cds", "sequence": "contig_1", "start": 595677, "stop": 595952, "strand": "-", "frame": 1, "gene": "ulaC", "product": "Ascorbate-specific PTS system EIIA component", "db_xrefs": [ "COG:COG1762", "COG:GT", "GO:0005737", "GO:0009401", "GO:0016301", "SO:0001217", "UniRef:UniRef50_P75292", "UniRef:UniRef90_P75292" ], "nt": "ATGCCTCATGCCCAGAATAACGGTACGGTACAGCAAAACTGCTTCTCTTTAGTGACCCTAAAGGAACCAGTTTACTTTGACAACGATCCCCGCCCGGTGCGCTTGTTAATTGGATTAGCGGCTACCAGCGCTGCCATTCATACGACAGAAGCTTTACCACAAATTGCGGCCCTAGTAGAAGATACTCAAGTGGTTAAGGACTTGTTGGACTGTTGTGATGAGACCAGCTTGTTTGCTGTGATTGATCGGGTTAACTTACACAAATATTTAACTTAA", "aa": "MPHAQNNGTVQQNCFSLVTLKEPVYFDNDPRPVRLLIGLAATSAAIHTTEALPQIAALVEDTQVVKDLLDCCDETSLFAVIDRVNLHKYLT", "aa_hexdigest": "2ccbafedad258642cb98db42bf2d82eb", "start_type": "ATG", "rbs_motif": null, "genes": [ "ulaC" ], "truncated": "3-prime", "pseudogene": { "start": 595677, "stop": 596156, "observations": { "mutation": [ 596084 ], "stop": [ 596084 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75292", "query_cov": 1.0, "subject_cov": 0.5723270440251572, "identity": 1.0, "score": 186.0, "evalue": 7.85e-59, "gene_start": 1, "gene_end": 91, "reference_start": 69, "reference_end": 159, "reference_sequence": "MTKLDFKAVLKQHHTVRLHQTAQDWKQAIQLCIHPLIAAKLVQPAYFDDILKSVAQYGPYFIIAENVAMPHAQNNGTVQQNCFSLVTLKEPVYFDNDPRPVRLLIGLAATSAAIHTTEALPQIAALVEDTQVVKDLLDCCDETSLFAVIDRVNLHKYLT" }, "paralog": false, "identity": 0.9937106918238994, "score": 316.0, "evalue": 1.19e-112, "description": "Internal stop codon at 596084. Nonsense mutation around 596084.", "psc": { "uniref90_id": "UniRef90_P75292", "gene": "ulaC", "product": "Ascorbate-specific PTS system EIIA component", "uniref50_id": "UniRef50_P75292", "cog_id": "COG1762", "cog_category": "GT", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75292", "UniRef:UniRef50_P75292", "COG:COG1762", "COG:GT", "GO:0005737", "GO:0016301", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75292", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75292" ], "product": "Ascorbate-specific PTS system EIIA component" } }, "id": "CNOCLNGIAL_1060", "locus": "GCA000143945_01056" }, { "type": "cds", "sequence": "contig_1", "start": 596158, "stop": 596445, "strand": "-", "frame": 3, "gene": "ulaB", "product": "Ascorbate-specific PTS system EIIB component", "db_xrefs": [ "COG:COG3414", "COG:G", "EC:2.7.1.194", "GO:0005737", "GO:0008982", "GO:0009401", "GO:0016301", "RefSeq:WP_014325567.1", "SO:0001217", "UniParc:UPI0001DD6405", "UniRef:UniRef100_A0AAX0SP99", "UniRef:UniRef50_Q9EXD8", "UniRef:UniRef90_Q9EXD8" ], "nt": "ATGGAAAATAAGAAATTACACATTATTGCTGCTTGTGGTAACGGCATGGGTACGAGCATGCTAATCAAGATTAAGGTTGAAAAGATTATGAAGGAGTTGGGCTACACCGCCAAAGTGGAAGCTTTGTCAATGGGACAAACCAAGGGGATGGAGCACAGCGCTGACATTATTATTTCCTCGATTCACTTAACGAGTGAGTTTAACCCGAATGCTAAGGCCAAGATTGTTGGGGTGTTAAACCTGATGGATGAAAACGAAATTAAGCAAGCGTTAAGTAAGGTGCTTTAA", "aa": "MENKKLHIIAACGNGMGTSMLIKIKVEKIMKELGYTAKVEALSMGQTKGMEHSADIIISSIHLTSEFNPNAKAKIVGVLNLMDENEIKQALSKVL", "aa_hexdigest": "485936463dac84eda5cb31773b764513", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6405", "ncbi_nrp_id": "WP_014325567.1", "uniref100_id": "UniRef100_A0AAX0SP99", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6405", "RefSeq:WP_014325567.1", "UniRef:UniRef100_A0AAX0SP99" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SP99", "uniref90_id": "UniRef90_Q9EXD8" }, "psc": { "uniref90_id": "UniRef90_Q9EXD8", "gene": "ulaB", "product": "Ascorbate-specific PTS system EIIB component", "ec_ids": [ "2.7.1.194" ], "uniref50_id": "UniRef50_Q9EXD8", "cog_id": "COG3414", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD8" ], "product": "Ascorbate-specific PTS system EIIB component" }, "genes": [ "ulaB" ], "id": "CNOCLNGIAL_1061", "locus": "GCA000143945_01057" }, { "type": "cds", "sequence": "contig_1", "start": 596448, "stop": 596564, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTACCGTTGGTTTAATCTTGCTGGCACAAATTATTGACAATGGTAAGCAAACTAAAGTAACTAAATTACAACAATTACTCAAAATTAACCCACAATTAGATTTAAATAACTAA", "aa": "MATVGLILLAQIIDNGKQTKVTKLQQLLKINPQLDLNN", "aa_hexdigest": "ea7e76d9ecf550aeada399359f77c771", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4201.970200000001, "isoelectric_point": 9.524575233459473 }, "id": "CNOCLNGIAL_1062", "locus": "GCA000143945_01058" }, { "type": "cds", "sequence": "contig_1", "start": 596607, "stop": 597320, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGCCAGCTTAATTGCCATTATGACTGGGGTAAGGATGTTTATCACTGAGTTACAACAGGCGTTTCAAGGTATTAGTGAAAAGGTAGTACCAGGGGCCGTGGTAGCGGTTGATATTGCAGCTGTTTATGGGTTTTCAATTAACTCCGTGACCTTTGGGTTTTTAAGTGGGGTCATTGGTCAGTTTTTAGCTGTTGCCATTATGGCTGGCATTAGCTATATTCCGGGTAACCAGTTTAGCTTTGTGGCGATTCCTTTGTTTATTACACTCTTCTTTAACAGTGGTGCCTTTGGCGTGTATGCTAATGCCGAGGGTGGTTGGAAGGCGGCCTTACTGCTCCCGGGTATTATTGGCTTTTTAGAAATTATTGTGATTTCATTTGCGCTGCGCACCGTATCAAACGCTTACCAGGCTTCGGCACTGTTAGATGCTAAACAAAGCTTTGATTTAAAGGCGTTAATGGGTAATAGTTTGGACAATAACAACGGTTCGGCATTAAAAACAGCCGTAGAAAAGGTTGTAAATGCTACTGGTGTTAACCGGATAACCGAAGCGCAAAGCTTTGTTAAATCCGATAGCTTTAACAGTTTAAAAGCTGTTAACAGTACGCTAGCACAAGCAATTGAGTGGATCAGTAAAAGTGCATCACCCGTTGACAACGGTTTTATTGGAATGGCTGACTGGAATCTTTACTTTGGGTTGTTAGTTTGA", "aa": "MVASLIAIMTGVRMFITELQQAFQGISEKVVPGAVVAVDIAAVYGFSINSVTFGFLSGVIGQFLAVAIMAGISYIPGNQFSFVAIPLFITLFFNSGAFGVYANAEGGWKAALLLPGIIGFLEIIVISFALRTVSNAYQASALLDAKQSFDLKALMGNSLDNNNGSALKTAVEKVVNATGVNRITEAQSFVKSDSFNSLKAVNSTLAQAIEWISKSASPVDNGFIGMADWNLYFGLLV", "aa_hexdigest": "fa2c958b629bea6f4d56c8ada9842548", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25030.702300000004, "isoelectric_point": 5.06540012359619 }, "id": "CNOCLNGIAL_1063", "locus": "GCA000143945_01059" }, { "type": "cds", "sequence": "contig_1", "start": 597378, "stop": 597857, "strand": "-", "frame": 1, "gene": null, "product": "PTS ascorbate transporter subunit IIC", "db_xrefs": [ "KEGG:K03475", "SO:0001217", "UniRef:UniRef50_UPI002244F519", "UniRef:UniRef90_UPI002244F519" ], "nt": "ATGGTTACTGGACACGTTATGTTGCAACAAGCAGCGGTAGTTACTACGCTGTTCTATATTGTCTTGTTCCGCCAAATTCCGTTGTTGGGTACGGGCATTGCTTACGGTGCCCAAGCCGGTCTAGTGATTATTAGTGGTATCTTTTTGGGAGTTTACTGGTCTACGGCCTCTACGGGTACCTATTTAGTGACCAATAAGGTAACCAATAATGCTGGTTTTTCGATTGGTCACCAGCAGATGTTAGGGATTATGACGGTTGCTAAATTGGGCAAGTACTTTGGTGACAAAAACGATAGTGCCGAGCACAAAAAACTGCCTAAGGCCCTAAAGATCTTTGAAGATAATATCTTCACCCAAACGATCATTATCCTTAGTTTGTTTGTGGTGTTGTTTATTGTGATTTTGGCTAGTTACAAGGGTGATAAACCACTGTTAATTAACTGGGACAAATTTGGTGCCATTAATGGTTTAGAGATTTGA", "aa": "MVTGHVMLQQAAVVTTLFYIVLFRQIPLLGTGIAYGAQAGLVIISGIFLGVYWSTASTGTYLVTNKVTNNAGFSIGHQQMLGIMTVAKLGKYFGDKNDSAEHKKLPKALKIFEDNIFTQTIIILSLFVVLFIVILASYKGDKPLLINWDKFGAINGLEI", "aa_hexdigest": "c5228fc0191eff5d5115782c24e927bd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 597422, "stop": 598157, "observations": { "insertion": [ 597431 ], "mutation": [ 597878 ], "stop": [ 597878 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002244F519", "query_cov": 0.8930817610062893, "subject_cov": 0.42388059701492536, "identity": 1.0, "score": 270.0, "evalue": 1.29e-88, "gene_start": 1, "gene_end": 142, "reference_start": 192, "reference_end": 333, "reference_sequence": "MLNKIKLQQKRKLIIGWSVFALINLVVILLTVLLRAGLPNLVSKGVTPFSAQAFGDAFIFLITRVYLDNFLRQPALLLGVITLIGYLALGRGGVQSVVGALKTVIGFILLSIGSGVLVSTARPVFDTIKGLGGTGVVLLDPYFSLASANDFFTNSFLNNDYVSLIAFSLLVGFIVNIIFVGLKRWTNTNSIMVTGHVMLQQAAVVTTLFYIVLFRQIPLLGTGIAYGAQAGLVIISGIFLGVYWSTASTGTYLVTNKVTNNAGFSIGHQQMLGIMTVAKLGKYFGDKNDSAEHKKLPKALKIFEDNIFTQTIIILSLFVVLFIVILASYKGDKHC" }, "paralog": false, "identity": 0.9959016393442623, "score": 464.0, "evalue": 3.14e-167, "description": "Internal stop codon at 597878. Frameshift due to insertion around 597431. Nonsense mutation around 597878.", "psc": { "uniref90_id": "UniRef90_UPI002244F519", "product": "PTS ascorbate transporter subunit IIC", "uniref50_id": "UniRef50_UPI002244F519", "kegg_orthology_id": "K03475", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002244F519", "UniRef:UniRef50_UPI002244F519", "KEGG:K03475" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002244F519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F519" ], "product": "PTS ascorbate transporter subunit IIC" } }, "id": "CNOCLNGIAL_1064", "locus": "GCA000143945_01060" }, { "type": "cds", "sequence": "contig_1", "start": 597876, "stop": 598355, "strand": "-", "frame": 1, "gene": null, "product": "PTS ascorbate transporter subunit IIC", "db_xrefs": [ "KEGG:K03475", "SO:0001217", "UniRef:UniRef50_UPI002244F519", "UniRef:UniRef90_UPI002244F519" ], "nt": "GTGGTAATTCTCTTGACAGTATTACTGCGTGCAGGATTACCGAACTTAGTTAGTAAGGGAGTAACCCCGTTTTCGGCCCAAGCCTTTGGTGATGCCTTTATCTTTTTAATTACGCGGGTATACCTCGATAACTTTTTGCGTCAACCAGCGCTATTGTTAGGTGTTATTACCCTAATTGGTTACCTTGCTTTAGGTCGTGGTGGGGTGCAATCGGTGGTGGGGGCACTAAAGACCGTAATTGGCTTTATCCTGTTAAGCATTGGTAGTGGCGTGTTGGTGTCCACCGCCCGTCCCGTTTTTGACACGATTAAGGGTTTAGGGGGTACTGGTGTGGTACTATTGGATCCTTACTTTTCCTTAGCATCAGCGAACGATTTCTTTACGAACAGTTTCTTGAACAATGACTATGTGTCCTTGATTGCCTTTTCGCTGTTAGTGGGCTTTATTGTCAACATTATCTTTGTGGGGTTAAAGCGTTGA", "aa": "MVILLTVLLRAGLPNLVSKGVTPFSAQAFGDAFIFLITRVYLDNFLRQPALLLGVITLIGYLALGRGGVQSVVGALKTVIGFILLSIGSGVLVSTARPVFDTIKGLGGTGVVLLDPYFSLASANDFFTNSFLNNDYVSLIAFSLLVGFIVNIIFVGLKR", "aa_hexdigest": "727f362a7aa2c1218afdd2e34c123151", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 597573, "stop": 598430, "observations": { "mutation": [ 597878, 598382 ], "stop": [ 597878, 598382 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002244F519", "query_cov": 0.9937106918238994, "subject_cov": 0.4716417910447761, "identity": 1.0, "score": 288.0, "evalue": 9.59e-96, "gene_start": 2, "gene_end": 159, "reference_start": 27, "reference_end": 184, "reference_sequence": "MLNKIKLQQKRKLIIGWSVFALINLVVILLTVLLRAGLPNLVSKGVTPFSAQAFGDAFIFLITRVYLDNFLRQPALLLGVITLIGYLALGRGGVQSVVGALKTVIGFILLSIGSGVLVSTARPVFDTIKGLGGTGVVLLDPYFSLASANDFFTNSFLNNDYVSLIAFSLLVGFIVNIIFVGLKRWTNTNSIMVTGHVMLQQAAVVTTLFYIVLFRQIPLLGTGIAYGAQAGLVIISGIFLGVYWSTASTGTYLVTNKVTNNAGFSIGHQQMLGIMTVAKLGKYFGDKNDSAEHKKLPKALKIFEDNIFTQTIIILSLFVVLFIVILASYKGDKHC" }, "paralog": false, "identity": 0.9929824561403509, "score": 537.0, "evalue": 3.88e-196, "description": "Internal stop codon at 597878, 598382. Nonsense mutation around 597878, 598382.", "psc": { "uniref90_id": "UniRef90_UPI002244F519", "product": "PTS ascorbate transporter subunit IIC", "uniref50_id": "UniRef50_UPI002244F519", "kegg_orthology_id": "K03475", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002244F519", "UniRef:UniRef50_UPI002244F519", "KEGG:K03475" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002244F519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F519" ], "product": "PTS ascorbate transporter subunit IIC" } }, "id": "CNOCLNGIAL_1065", "locus": "GCA000143945_01061" }, { "type": "cds", "sequence": "contig_1", "start": 598430, "stop": 598573, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F32274", "UniRef:UniRef100_A0AB38W6G9", "UniRef:UniRef50_A0AB38W6G9", "UniRef:UniRef90_A0AB38W6G9" ], "nt": "GTGGGAGTTTCCGATGCGGCCATTAACACCATCTTGGTCGAAAATCCCGCTGAAATCCTGGCCTTTGATGCACCGCGGAAGTTTAATCCCAAGGCTGTGCATCCACGGGTATTAGCACTTAAAAAACAACTAAAAATAGAGTAA", "aa": "MGVSDAAINTILVENPAEILAFDAPRKFNPKAVHPRVLALKKQLKIE", "aa_hexdigest": "d2481e296c7fa3b06f9740f390960dd9", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002F32274", "uniref100_id": "UniRef100_A0AB38W6G9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F32274", "UniRef:UniRef100_A0AB38W6G9" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6G9", "uniref90_id": "UniRef90_A0AB38W6G9" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6G9", "uniref50_id": "UniRef50_A0AB38W6G9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6G9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5168.0659, "isoelectric_point": 9.397636985778806 }, "id": "CNOCLNGIAL_1066", "locus": "GCA000143945_01062" }, { "type": "cds", "sequence": "contig_1", "start": 598639, "stop": 598794, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FEEE66", "UniRef:UniRef100_A0AB38W6Q4", "UniRef:UniRef50_A0AB38W6Q4", "UniRef:UniRef90_A0AB38W6Q4" ], "nt": "GTGACCCTGTGTTTTGATGGGCCCGATCGGGTGAAGTATTACCCCGATTGTTTATTGGCTAAACACATTAAATACTTAGTGGACTTGGGCTTTGTTAAACACATTACCCTGGCCTTAGATGCGGGGCGGGTACTGTACCAAAACACTATGGTTTAG", "aa": "MTLCFDGPDRVKYYPDCLLAKHIKYLVDLGFVKHITLALDAGRVLYQNTMV", "aa_hexdigest": "4c2ee55de2c9cbfbff0f2f645c7c6ee5", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002FEEE66", "uniref100_id": "UniRef100_A0AB38W6Q4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FEEE66", "UniRef:UniRef100_A0AB38W6Q4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6Q4", "uniref90_id": "UniRef90_A0AB38W6Q4" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6Q4", "uniref50_id": "UniRef50_A0AB38W6Q4" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6Q4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5859.9022, "isoelectric_point": 7.79804744720459 }, "id": "CNOCLNGIAL_1067", "locus": "GCA000143945_01063" }, { "type": "cds", "sequence": "contig_1", "start": 598858, "stop": 599289, "strand": "-", "frame": 3, "gene": null, "product": "Phosphotriesterase-related protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00023869D4", "UniRef:UniRef100_A0AAV5N8W9", "UniRef:UniRef50_A0AAV5N8W9", "UniRef:UniRef90_A0AAV5N8W9" ], "nt": "ATGGATCCACCGAATGTGGGCCGTGATGTCAAACGGATGGTAGCGATTGCAGAACAACTAAAGGGCAAGCTGAACATTATTATGGCCACTGGTTTTCACAAGGCTGCTTTCTATGACAAGGGAAGTTCTTGGTTAGCACAAGTGCCAGTTAATGAAATTGTGCCGATGTTGGTCGCTGAAATAGAAGAGGGTATGGATTTGTATAACTATTCCGGTCCCGTGGTTAAACGGGGCAAGGCTAAGGCAGGCATTATTAAAGCTGGTACGGGCTATGCTGCGATTGACCGGTTGGAATTAAAGGCCTTGGAAGCAGTCGCGATTACTTCGATTACTACTGGTGCTCCCGTACTCGTGCACACCCAACTCGGTACCATGGCATATGAAGCAGTACAACACCTGATTGATTTTGGCGTTAATCCGCGGAAAATTTAA", "aa": "MDPPNVGRDVKRMVAIAEQLKGKLNIIMATGFHKAAFYDKGSSWLAQVPVNEIVPMLVAEIEEGMDLYNYSGPVVKRGKAKAGIIKAGTGYAAIDRLELKALEAVAITSITTGAPVLVHTQLGTMAYEAVQHLIDFGVNPRKI", "aa_hexdigest": "505079639ef0fffb8c756657b2841ab0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00023869D4", "uniref100_id": "UniRef100_A0AAV5N8W9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00023869D4", "UniRef:UniRef100_A0AAV5N8W9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N8W9", "uniref90_id": "UniRef90_A0AAV5N8W9" }, "psc": { "uniref90_id": "UniRef90_A0AAV5N8W9", "product": "Phosphotriesterase-related protein", "uniref50_id": "UniRef50_A0AAV5N8W9" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N8W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N8W9" ], "product": "Phosphotriesterase-related protein" }, "genes": [], "id": "CNOCLNGIAL_1068", "locus": "GCA000143945_01064" }, { "type": "cds", "sequence": "contig_1", "start": 599323, "stop": 599484, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_497", "db_xrefs": [ "GO:0008270", "GO:0009056", "GO:0016787", "SO:0001217", "UniParc:UPI0001DD6406", "UniRef:UniRef100_P75290", "UniRef:UniRef50_Q4A724", "UniRef:UniRef90_P75290" ], "nt": "ATGAAAAGATTTGTAAGAACAGTTTTAGGGGACATTGACCCTAAGGATTTAGGTATTTGTGACTGTCACGATCATTTAATTAAGAATTGGGGTCCGGAAGCCAAAGAGCACCCTGATTTTGTGATGTTATCGAATGAAGCTGCTATTAAAGAGTGTTTGTAA", "aa": "MKRFVRTVLGDIDPKDLGICDCHDHLIKNWGPEAKEHPDFVMLSNEAAIKECL", "aa_hexdigest": "72db58d0e68b6a7a87116caca0a28e08", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6406", "uniref100_id": "UniRef100_P75290", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6406", "UniRef:UniRef100_P75290" ] }, "ips": { "uniref100_id": "UniRef100_P75290", "uniref90_id": "UniRef90_P75290" }, "psc": { "uniref90_id": "UniRef90_P75290", "product": "Uncharacterized protein MPN_497", "uniref50_id": "UniRef50_Q4A724", "go_ids": [ "GO:0016787", "GO:0008270", "GO:0009056" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A724", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A724" ], "product": "Phospho-furanose lactonase" }, "genes": [], "id": "CNOCLNGIAL_1069", "locus": "GCA000143945_01065" }, { "type": "cds", "sequence": "contig_1", "start": 599600, "stop": 600328, "strand": "+", "frame": 2, "gene": "ulaF", "product": "putative L-ribulose-5-phosphate 4-epimerase UlaF", "db_xrefs": [ "COG:COG0235", "COG:EG", "EC:5.1.3.4", "GO:0005829", "GO:0008270", "GO:0008742", "GO:0016832", "GO:0019323", "GO:0019852", "GO:0019854", "RefSeq:WP_014325569.1", "SO:0001217", "UniParc:UPI0001DD6407", "UniRef:UniRef100_A0AAX0S215", "UniRef:UniRef50_P75289", "UniRef:UniRef90_P75289" ], "nt": "ATGGATCAAAAAATGATAAATGACCTCAAAGAACAGGTTTTTCAAACGAATTTATTACTACCCAAATATGGCTTAGTCATCCACACTTGGGGTAATGTTTCGATGATTGCCCCGAACCGTCAGTTCTTTGTCATTAAACCAAGTGGTGTGAGCTATGACAAGATGCGTGCCCAAGATATGGTAGTGGTCGATTTGGACAACAATGTTCTAGATACCAACGGCCTCAAACCATCAAGTGATACCCCAACCCATGCGTTAATGTACAAACATTGTCCAGATATTAAAGCTATTGTGCACACCCACTCTACTTTAGCGACATCGTTTGCTCAAGCCGATAAGCCGATTCCTTGTCTTGGTACTACCCATGCTGATAACTTCTTTGGACCAATCCCGTGTACTAGGGCTTTAAGTGACAGTGAAATTAATGGAGCTTATGAACATAATACTGGTTTAGTCATTCTGGAACACCTCAAAAACAACCAGGTTGATGTCAATGCTTGTGCGGCCATCTTAGTCAAAGAACACGGTTCGTTTGTTTGGTCCAATAAGAATGGCAAGGATGCAGTGGACCGGGCGTTAACTTTAGAACAAGTGGCCCAAATGGCCTTATATACCCAAATGATCAATCCCCACATGAAAGAAGCAAACCCAGCGTTACAACAAAAGCACTATAACCGCAAACATGGTAAAGATGCTTACTATGGACAAAATACTAAGCAAGAAGATTAA", "aa": "MDQKMINDLKEQVFQTNLLLPKYGLVIHTWGNVSMIAPNRQFFVIKPSGVSYDKMRAQDMVVVDLDNNVLDTNGLKPSSDTPTHALMYKHCPDIKAIVHTHSTLATSFAQADKPIPCLGTTHADNFFGPIPCTRALSDSEINGAYEHNTGLVILEHLKNNQVDVNACAAILVKEHGSFVWSNKNGKDAVDRALTLEQVAQMALYTQMINPHMKEANPALQQKHYNRKHGKDAYYGQNTKQED", "aa_hexdigest": "e1ac05b81cef137a8c8257c0649af3bd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6407", "ncbi_nrp_id": "WP_014325569.1", "uniref100_id": "UniRef100_A0AAX0S215", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6407", "RefSeq:WP_014325569.1", "UniRef:UniRef100_A0AAX0S215" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S215", "uniref90_id": "UniRef90_P75289" }, "psc": { "uniref90_id": "UniRef90_P75289", "gene": "ulaF", "product": "Probable L-ribulose-5-phosphate 4-epimerase UlaF", "ec_ids": [ "5.1.3.4" ], "uniref50_id": "UniRef50_P75289", "cog_id": "COG0235", "cog_category": "EG", "go_ids": [ "GO:0005829", "GO:0016832", "GO:0008742", "GO:0008270", "GO:0019854", "GO:0019852", "GO:0019323" ] }, "pscc": { "uniref50_id": "UniRef50_P75289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75289" ], "product": "Probable L-ribulose-5-phosphate 4-epimerase UlaF" }, "genes": [ "ulaF" ], "id": "CNOCLNGIAL_1070", "locus": "GCA000143945_01066" }, { "type": "cds", "sequence": "contig_1", "start": 600282, "stop": 600773, "strand": "+", "frame": 3, "gene": null, "product": "MPN499 family protein", "db_xrefs": [ "RefSeq:WP_010874855.1", "SO:0001217", "UniParc:UPI000013AB8A", "UniRef:UniRef100_P75288", "UniRef:UniRef50_P75288", "UniRef:UniRef90_P75288" ], "nt": "ATGGTAAAGATGCTTACTATGGACAAAATACTAAGCAAGAAGATTAAAGTGAACTGGTTGGGCGGGGTGTTTTGGTTATTACCTAACTTACTCGATCTGTTTTCCGCTAGCAAACGTAAAGCGAGTGTGCGACCCTACCAAAGCTTATTGGAACTGGTTCAAGAAAACTTCTTGAACCGGTATGACCTCGTGCACTTTAGCTTTAATGGTGACCATGACTTCTTTCACTTTAATGATTTACAAGCCATTAGGAGCTTTAACTTTACGATAGAAGAGGAACAACTAGGAGCAATGCAACCAGATGAGGTTTTACTGTTTGAACCTGTGGATCGTGTAACAGTGGAATTAGATCAAAAGGGTTTGAGTTTAATCCACAGTGGCAAAGCTTTTTGTGCTAGTGCAAACTACTTTAAGCACTGGCTAAAACGCGTACCACAGCAAGACAAAGTGACCCTAGTGTGGCGAAAATCAGGTTTTGAATTAAAGCAGTAG", "aa": "MVKMLTMDKILSKKIKVNWLGGVFWLLPNLLDLFSASKRKASVRPYQSLLELVQENFLNRYDLVHFSFNGDHDFFHFNDLQAIRSFNFTIEEEQLGAMQPDEVLLFEPVDRVTVELDQKGLSLIHSGKAFCASANYFKHWLKRVPQQDKVTLVWRKSGFELKQ", "aa_hexdigest": "9c705784d6f0e311001497b1cc97e276", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AB8A", "ncbi_nrp_id": "WP_010874855.1", "uniref100_id": "UniRef100_P75288", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AB8A", "RefSeq:WP_010874855.1", "UniRef:UniRef100_P75288" ] }, "ips": { "uniref100_id": "UniRef100_P75288", "uniref90_id": "UniRef90_P75288" }, "psc": { "uniref90_id": "UniRef90_P75288", "product": "MPN499 family protein", "uniref50_id": "UniRef50_P75288" }, "pscc": { "uniref50_id": "UniRef50_P75288", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75288" ], "product": "Uncharacterized protein MPN_499" }, "genes": [], "id": "CNOCLNGIAL_1071", "locus": "GCA000143945_01067" }, { "type": "cds", "sequence": "contig_1", "start": 600762, "stop": 601118, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAGGGTGGGTCATTTGGGAATATTGTTCCCCGTAGTCGTCGTCTGACTGCCCCCGAGGGTGCCCTGGACATTCGCTTTGAGGCCAACGCCGACATTCGAAAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCTGGTTTGAGGCGCGGTTATATCATCCACACCCCGGTGGTTATTCCCATACCTGTACCCATTCCCAATCCAGGGAATATAGTCCAACACCTGGTCGAGCACCGACCCCGCGAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGCTATATAGTATTAGAGTAATTCTACTGCTTTAA", "aa": "MVEGGSFGNIVPRSRRLTAPEGALDIRFEANADIRKRREKGTRTTCVRTSSAGRPRAGLRRGYIIHTPVVIPIPVPIPNPGNIVQHLVEHRPREVEPGGETGKRGGEVLYSIRVILLL", "aa_hexdigest": "3493e9d32e5569d42c9a76f124f0b156", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12977.882299999988, "isoelectric_point": 11.19572467803955 }, "id": "CNOCLNGIAL_1072", "locus": "GCA000143945_01068" }, { "type": "cds", "sequence": "contig_1", "start": 601485, "stop": 601775, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNKSGQDDNSKFKAEDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRASGSGSNTTSSPGIGFKIPEQSGTNTTSKAVLITPGLA", "aa_hexdigest": "890f22afdbdbb58513e9f1ad165ff405", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10171.074699999988, "isoelectric_point": 5.170722770690916 }, "id": "CNOCLNGIAL_1073", "locus": "GCA000143945_01069" }, { "type": "cds", "sequence": "contig_1", "start": 601998, "stop": 602501, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593", "UniRef:UniRef90_UPI001330D5D0" ], "nt": "GTGGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTATACCGACAAGGATTTGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCCCTACCTCCATTTTATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGAGGAGTAGTGGTAACCTCAGTAGTGTGTTTAGTGGTAGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAA", "aa": "MESVWDFKGVWADQAQLAAQAATSSTTTTATGATLPEHPNALAYQISYTDKDLYKASTQGSGQTNSQNNSPYLHFIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGRSSGNLSSVFSGRGAGGGSSGSGQSGVDLSPVERVSGH", "aa_hexdigest": "f024ade581ead5db44fe8f6d892c32ab", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 601698, "stop": 602501, "observations": { "mutation": [ 601773, 601956 ], "stop": [ 601773, 601956 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001330D5D0", "query_cov": 1.0, "subject_cov": 0.46005509641873277, "identity": 0.988, "score": 306.0, "evalue": 4.83e-102, "gene_start": 1, "gene_end": 167, "reference_start": 197, "reference_end": 363, "reference_sequence": "ALSFTNKNNAQRAQLFLRGLLGSIPVLVNKSGQDDNSKFKAEDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRASGSGSNTTSSPGIGFKIPEQSGTNTTSKAVLITPGLAWTPQDVGNLVVSGTSFSFQLGGWLVTFTDFIKPRAGYLGLQLTGLDVSEATQRELIWAKRPWAAFRGSWVNRLGRVESVWDFKGVWADQAQLAAQAATSSTTTTATGATLPEHPNALAYQISYTDKDLYKASTQGSGQTNSQNNSPYLHFIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGTSSGNLSSVFSGRGAGGGSSGSGQSGVDLSPVERVSGH" }, "paralog": false, "identity": 0.9887640449438202, "score": 513.0, "evalue": 5.65e-186, "description": "Internal stop codon at 601773, 601956. Nonsense mutation around 601773, 601956.", "psc": { "uniref90_id": "UniRef90_UPI001330D5D0", "product": "adhesin", "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001330D5D0", "UniRef:UniRef50_P75593" ] }, "pscc": { "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593" ], "product": "Putative adhesin P1-like protein MPN_099" } }, "id": "CNOCLNGIAL_1074", "locus": "GCA000143945_01070" }, { "type": "cds", "sequence": "contig_1", "start": 602568, "stop": 602867, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640A", "UniRef:UniRef100_A0A0H3DNZ5", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0A0H3DNZ5" ], "nt": "GTGTCTTTACCAACTTTAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGGCTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTGTTGGCGCTTCTCAATAAACTGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAA", "aa": "MSLPTLSNLVNEYPKSMKLVKLARLHRFLKQDQLQLVPLFSPNFLQVLLALLNKLVNLFSENTTMKTNKKYAFAALLMQAQAKLPNSFKFFLSKKGGMA", "aa_hexdigest": "9cdb1adeccaa0056dddaece30460748d", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD640A", "uniref100_id": "UniRef100_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640A", "UniRef:UniRef100_A0A0H3DNZ5" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DNZ5", "uniref90_id": "UniRef90_A0A0H3DNZ5" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DNZ5", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11274.444899999984, "isoelectric_point": 10.390707969665527 }, "id": "CNOCLNGIAL_1075", "locus": "GCA000143945_01071" }, { "type": "cds", "sequence": "contig_1", "start": 603035, "stop": 603448, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTTAACGAGTACAAAAACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGCGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGCTTGGACAAGATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAGAAAAGAATAGACAAACTTGA", "aa": "MNTEYVTRKEFNEYKNSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQIEQKAQGETLKLILQTLQKMSDRLDKMEVKMDKMEVKMDKMEVKMDKMEEKNRQT", "aa_hexdigest": "88802c7c06eb768e17a2572c1fc09c10", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 54, "aa_cov": 0.39416058394160586, "hmm_cov": 1.0, "evalue": 8.834398831080154e-38, "score": 124.49153137207031, "start": 2, "stop": 55 } ], "seq_stats": { "molecular_weight": 16144.400699999998, "isoelectric_point": 5.980109977722168 }, "id": "CNOCLNGIAL_1076", "locus": "GCA000143945_01072" }, { "type": "cds", "sequence": "contig_1", "start": 603903, "stop": 604160, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGATAACATTGCCAAGGGCATCACTGCCGGTAGTAACACCCAACAAACCACGTATGATCCCACCCGAACCGAGGCCACCTTGACCACCGCCACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGGCTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNIAKGITAGSNTQQTTYDPTRTEATLTTATTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "f069261336431c534981970471d38417", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 5.089856120031307e-09, "score": 31.335514068603516, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9232.065799999997, "isoelectric_point": 8.137860298156738 }, "id": "CNOCLNGIAL_1077", "locus": "GCA000143945_01073" }, { "type": "cds", "sequence": "contig_1", "start": 604191, "stop": 604700, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_502", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571", "UniRef:UniRef90_P75285" ], "nt": "GTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAAAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCTATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGYESKSSNGSSSTSTTQRGGSSNENKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTP", "aa_hexdigest": "780379d1e8039b126587944a38c67f1a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 603903, "stop": 605003, "observations": { "mutation": [ 604158, 604698, 604860, 604875, 604899 ], "stop": [ 604158, 604698, 604860, 604875, 604899 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75285", "query_cov": 1.0, "subject_cov": 0.4004739336492891, "identity": 0.9940000000000001, "score": 255.0, "evalue": 2.89e-81, "gene_start": 1, "gene_end": 169, "reference_start": 97, "reference_end": 265, "reference_sequence": "MRDNIAKGITAGSNTQQTTYDPTRTEATLTTATTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKDKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGYESKSSNGSSSTSTTQRGGSSNENKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTPWTPWLTTEQIHNDPAKFAASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFEQALLVFGICLS" }, "paralog": false, "identity": 0.9836065573770492, "score": 662.0, "evalue": 4.58e-244, "description": "Internal stop codon at 604158, 604698, 604860, 604875, 604899. Nonsense mutation around 604158, 604698, 604860, 604875, 604899.", "psc": { "uniref90_id": "UniRef90_P75285", "product": "Uncharacterized protein MPN_502", "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75285", "UniRef:UniRef50_P75571" ] }, "pscc": { "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571" ], "product": "Uncharacterized protein MPN_205" } }, "id": "CNOCLNGIAL_1078", "locus": "GCA000143945_01074" }, { "type": "cds", "sequence": "contig_1", "start": 604917, "stop": 605171, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGAGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAA", "aa": "MLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFEQALLVFGICLS", "aa_hexdigest": "451778bce0a189a6b072b668f7a1372b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 77, "aa_cov": 0.9166666666666666, "hmm_cov": 0.6623376623376623, "evalue": 1.953872690982626e-17, "score": 58.95265197753906, "start": 4, "stop": 80 } ], "seq_stats": { "molecular_weight": 9421.290499999997, "isoelectric_point": 4.418060874938965 }, "id": "CNOCLNGIAL_1079", "locus": "GCA000143945_01075" }, { "type": "cds", "sequence": "contig_1", "start": 605316, "stop": 605780, "strand": "+", "frame": 3, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_A0AB38W808" ], "nt": "GTGGAGGATACCAAGTTGGTGGCGCTGGTCCGTTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGA", "aa": "MEDTKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPKATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYKTLQDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLEQTTQ", "aa_hexdigest": "ce32da2debf037f96e7dbed7d4371f00", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 605265, "stop": 605952, "observations": { "insertion": [ 605895 ], "mutation": [ 605307, 605778, 605886 ], "stop": [ 605307, 605778, 605886 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W808", "query_cov": 0.948051948051948, "subject_cov": 0.6160337552742616, "identity": 0.9790000000000001, "score": 272.0, "evalue": 7.58e-91, "gene_start": 5, "gene_end": 150, "reference_start": 21, "reference_end": 166, "reference_sequence": "MRAAHLHTSYELVDWKVGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESGWDNQRSQKNLTNNPGPKAVTGFKLDKGRAYRKLNESWPVSNPSIRPRLARGKVPQNEKRRRQMLRVMLP" }, "paralog": false, "identity": 0.8596491228070176, "score": 367.0, "evalue": 1.61e-130, "description": "Internal stop codon at 605307, 605778, 605886. Frameshift due to insertion around 605895. Nonsense mutation around 605307, 605778, 605886.", "psc": { "uniref90_id": "UniRef90_A0AB38W808", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W808", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "CNOCLNGIAL_1080", "locus": "GCA000143945_01076" }, { "type": "cds", "sequence": "contig_1", "start": 605991, "stop": 606218, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MSSTESQMAAVTDSQQSGHNSGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "01a905cfd228e3b6c2671a39b4f01a08", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7844.479600000001, "isoelectric_point": 5.985339164733887 }, "id": "CNOCLNGIAL_1081", "locus": "GCA000143945_01077" }, { "type": "cds", "sequence": "contig_1", "start": 606477, "stop": 607034, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI001FCE6DDF" ], "nt": "TTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCTTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCAAAGATTGCCTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTTGA", "aa": "MNWGTDKQKQFVENQLGFKDDSNSSLTNFKSQGLTQPAYLIAGLDVVQDHLVFAAFKAGAVGYDMTTDSNASTKDQALAWSTTAGLDSAGGYNNLVENTAGLNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGDKKAIYPEVRGRQLQNIF", "aa_hexdigest": "e3d23938cf56cea06e957840f36290b2", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCE6DDF", "query_cov": 1.0, "subject_cov": 0.8409090909090909, "identity": 0.9890000000000001, "score": 367.0, "evalue": 5.03e-128, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "CNOCLNGIAL_1082", "locus": "GCA000143945_01078" }, { "type": "cds", "sequence": "contig_1", "start": 607440, "stop": 607550, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAGAAAATTTCCGAAAAGGAATACAAAGCTTTAATCCGTAAAACTGGCAAAGAGCACTTTGATGGTGAAAAAGAAGAGTATGGTGATGGCACAGTTGGAGTTTGA", "aa": "MKEKISEKEYKALIRKTGKEHFDGEKEEYGDGTVGV", "aa_hexdigest": "435a21db0dde7c895a3c3aec2dc0ec06", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4130.589700000002, "isoelectric_point": 5.72024211883545 }, "id": "CNOCLNGIAL_1083", "locus": "GCA000143945_01079" }, { "type": "cds", "sequence": "contig_1", "start": 607587, "stop": 607820, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAAAGTTAAATACGTAACCCAAGAACAATTTCAGGAATATAAAGATTCCAACAATCAGCGACTCATAAAGATTGAAAATAAGGTTGATAAATTAGTTGAAATTGTTCAAATTCACGGCGAACAAATTAAAGCACAGGGAGAAACCCTGCAATTAATTTTGCAAACGCTGCAAAAGATGAGTGATCGCTTGGACAAGATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAA", "aa": "MKVKYVTQEQFQEYKDSNNQRLIKIENKVDKLVEIVQIHGEQIKAQGETLQLILQTLQKMSDRLDKMEKRIDKLESK", "aa_hexdigest": "21002b18daba759e88e7f408c2bc702c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.8701298701298701, "hmm_cov": 1.0, "evalue": 3.9554233881977954e-20, "score": 67.77593231201172, "start": 2, "stop": 68 } ], "seq_stats": { "molecular_weight": 9203.579399999995, "isoelectric_point": 8.89162464141846 }, "id": "CNOCLNGIAL_1084", "locus": "GCA000143945_01080" }, { "type": "cds", "sequence": "contig_1", "start": 608044, "stop": 608286, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATACCAAAGTTTTAAGGGTTTGTCAATTGAAGCTAAGTTGAGCTATAACTTTAATTGGTTTGGAGATTATTCTTCAGGTGGTTTCACTGCTAAGAAAGGTGACAAGCATTATTTTGATCTTTTCCTTAAAATTAAATCTAATTCTGATCCAAAAAAACAATTTACTACAGAAAAATTTCTTACTAAAGAAGAAAAAAATAAAGTTAATGGAAAAGAAATTACAAGAAATTTAGAGTGA", "aa": "MTYQSFKGLSIEAKLSYNFNWFGDYSSGGFTAKKGDKHYFDLFLKIKSNSDPKKQFTTEKFLTKEEKNKVNGKEITRNLE", "aa_hexdigest": "87b6dd737675d38b306775e92f2c9848", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9344.441899999996, "isoelectric_point": 9.456109809875489 }, "id": "CNOCLNGIAL_1085", "locus": "GCA000143945_01081" }, { "type": "cds", "sequence": "contig_1", "start": 608401, "stop": 608649, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75316", "UniRef:UniRef90_P75316" ], "nt": "ATGCGCAAAAAGAGATTGTTGTCAAGGATTTCCTTTAGTTCTCTATTTTTATTATGTGGAACAGTTTTAAGCGCCTATACTGGCATCCAAGCAGATCTCAGAAATCTTATCAAGGAAACCACCAAAAAAGACATTGATGTTTACAAGGCAATTAAAACTACAGAAGGAAAAAAGAACATAATAACTTCTTTAAAGAAATCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGA", "aa": "MRKKRLLSRISFSSLFLLCGTVLSAYTGIQADLRNLIKETTKKDIDVYKAIKTTEGKKNIITSLKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "ed29bf6947df9f89923155db9a0c3d5a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 0.902, "score": 137.0, "evalue": 1.98e-40, "valid": true, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "CNOCLNGIAL_1086", "locus": "GCA000143945_01082" }, { "type": "cds", "sequence": "contig_1", "start": 608669, "stop": 608770, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAAATTTCAGTTTTTTAATTAATGTTCAAACCGAAATTTCAATTAGCTTTCCGTTTTTAAAGTTGCTTTTCCAAAAAAAAAAAAAACAAAATTAA", "aa": "MIENFSFLINVQTEISISFPFLKLLFQKKKKQN", "aa_hexdigest": "da93ad7975192f1c0f62108e2e9a6dfe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3974.7080000000014, "isoelectric_point": 9.82448215484619 }, "id": "CNOCLNGIAL_1087", "locus": "GCA000143945_01083" }, { "type": "cds", "sequence": "contig_1", "start": 609082, "stop": 609288, "strand": "+", "frame": 1, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640D", "UniRef:UniRef100_A0AAP8E4R5", "UniRef:UniRef50_A0AAP8E4D4", "UniRef:UniRef90_A0AAP8E4D4" ], "nt": "ATGATAGAAGTATTAAGTGATTCTGAAATTTTGTCTTATTTATTAAGTAATAAGCACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAG", "aa": "MIEVLSDSEILSYLLSNKHTADGVNEIYVTMNIKDKDSNTQRKPDKMSLELELFLNQFIEFLKEQRKS", "aa_hexdigest": "71d7b59af7957e717843d8a92e09190f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD640D", "uniref100_id": "UniRef100_A0AAP8E4R5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640D", "UniRef:UniRef100_A0AAP8E4R5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E4R5", "uniref90_id": "UniRef90_A0AAP8E4D4" }, "psc": { "uniref90_id": "UniRef90_A0AAP8E4D4", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D4" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4" ], "product": "Family K-like protein" }, "genes": [], "id": "CNOCLNGIAL_1088", "locus": "GCA000143945_01084" }, { "type": "cds", "sequence": "contig_1", "start": 609570, "stop": 610373, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_146", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140" ], "nt": "ATGAAATTTAAGTATGGTGCCATCTTTTTTAGTGGCTTTCTAGGTCTTTCTGCCATTTTGGCTGCCTGTGGTACAAAGGGTAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTCCTCTTTAACTTCTAAAAGTGCATCAAAAGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCCTATTGAAATTACCCAAATCGCTGGTGGTTATGATGGCGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAAGACACCACTAACTTTTACAACTTAATCTTAAACTACCCTGATCTAGTTTCAACTTTAGGTCGTGTTGGTATGGAACTGCCGTTTGACAATGTAAAGGTTGACAAACTATCACCCCGTTTTTTAGATTTCAACAACCGCATTAGTGCAATTTCTAAACCAGGAATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAACAACGCTAAAAAGAAAGAAGGTACTTTAAACCAAAAAATGACAAGTTCTTCTGAAGGAAAAAACAGCAGTGGCACTTTAACAGTAGCAACTGATACTGAAACAAGTAGTTTATGGAAAAAGATAGAAGATTCTGCAAAAGCTAACGGTAAAAGCGATGAAAAAGGAAAAGGTAAGAAGAAAGATAATAAAAGTGCAACTTTTTCGCTTGTACAACTAAAACAAACTCAAGAAAAAACAGATGATTCCCAAGACACTAAAAATAGTGATGATCAAGTTAAAAAATCTTGA", "aa": "MKFKYGAIFFSGFLGLSAILAACGTKGKFDQVDDGKIKLASSLTSKSASKALQAIVKKYNEVKKPGDYPIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELPFDNVKVDKLSPRFLDFNNRISAISKPGIYGIPVSLSTEVLSINGPVLHYILNNAKKKEGTLNQKMTSSSEGKNSSGTLTVATDTETSSLWKKIEDSAKANGKSDEKGKGKKKDNKSATFSLVQLKQTQEKTDDSQDTKNSDDQVKKS", "aa_hexdigest": "3e99355df59412f73486d842bc3f3442", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75140", "query_cov": 1.0, "subject_cov": 0.9434628975265018, "identity": 0.539, "score": 242.0, "evalue": 2.49e-76, "valid": false, "product": "Uncharacterized protein MPN_146", "uniref50_id": "UniRef50_P75140" }, "pscc": { "uniref50_id": "UniRef50_P75140", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140" ], "product": "Uncharacterized protein MPN_146" }, "genes": [], "id": "CNOCLNGIAL_1089", "locus": "GCA000143945_01085" }, { "type": "cds", "sequence": "contig_1", "start": 610563, "stop": 611567, "strand": "+", "frame": 3, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75280" ], "nt": "TTGTTTACCTCTGTTTTTGCAGCTGGCGGCGGGGATTACAATAATTTCTTTTACAAGATTGAAAATGGTCGCGCTGACTTTAGTAACTTTAAGAATAAAGGGACTTCTTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTCCAAAAGTTCCACCAATTAGCTTACAGCATTTCCTCTACTTCCGGTTTCTTCTATTCGTTTGCCGGTAAAAGTGCAAAGCGTTTAAATTTTGGTGATAGCTTTATTGAGTATCCGCGGTTTACTCAAGAAATTAAAGCACCATCAAAAAATGGAGAAAATGGCCAAACAAATGAAGGAAATAGTACCAATGGCGAACAAAATCTCTTAGGAACTTTCGAAGTCAAGGATGACAGTAAGCCTAAAGAAGAAGTTAAAAGTAATAAAAATAGTGGTAAAGAAAGTAGCCAAAATCAGGGTAAAAAATCTAACAATAACAAGACTATTTACCTTTATGAAACAAAAATTCCAGATGGAAAAACTGCAGGTGATAATGCTATCTTAATCAAAGATAAGAACGTTATCGAAAAGCTTAAAAGTGCAGCTAAAGAAGAAAATAAAGAACAAACTGCTGAAGCAACTAAAGCTGCAATTACAAGTAATAAAGCAAAAAGCACTAAAAAAGAAAGCAGTAAAGTTATCGGTTACACCACAACTGATAGTGTTCGCGAAGATGGCAAAAACATTTTCGCAATAGATAGAGTTAATGGTGAAAACTATGACCGTAAGATTATTGTAGGTGCTAAAGCAGAAACTTTAAACCAATCTAGCACCTTACAAAGTGAAGAAGCGATTGTTTTGCCTGCCCCTGGAAAGTATCTAAATGGTGATCCCAAGAAAGTGACAATTACCCAAGGACCTAACATCATTGGCATTCATGCTAACGAAAAGGAAAATGCAGAAACGCAAAAGTTCGTAGATTGA", "aa": "MFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGKSAKRLNFGDSFIEYPRFTQEIKAPSKNGENGQTNEGNSTNGEQNLLGTFEVKDDSKPKEEVKSNKNSGKESSQNQGKKSNNNKTIYLYETKIPDGKTAGDNAILIKDKNVIEKLKSAAKEENKEQTAEATKAAITSNKAKSTKKESSKVIGYTTTDSVREDGKNIFAIDRVNGENYDRKIIVGAKAETLNQSSTLQSEEAIVLPAPGKYLNGDPKKVTITQGPNIIGIHANEKENAETQKFVD", "aa_hexdigest": "f7e6ece2e523049b72374748f2386385", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 610263, "stop": 611870, "observations": { "mutation": [ 610371, 610440, 611565, 611589 ], "stop": [ 610371, 610440, 611565, 611589 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75280", "query_cov": 1.0, "subject_cov": 0.41802252816020025, "identity": 0.997, "score": 617.0, "evalue": 2.52e-215, "gene_start": 1, "gene_end": 334, "reference_start": 338, "reference_end": 671, "reference_sequence": "MKFKYCAIFFSGFLGLSAILAACGARGKFDQVDDGKIKLAFSLTSKSASNALQAIVKKYNEVKKPGDYHIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDLWEKIEASAKANGKSNKDGQTKGKKKVEKSSSSSLVNLKQSTDQTTTDDGSQKSDNKIKESWGEYQEVDGGLKNFEFKASIFENWHDLLDFSTRVAKSFKKIHENSNKKGNDIQGILGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGKSAKRLNFGDSFIEYPRFTQEIKAPSKNGENGQTNEGNSTNGEQNLLGTFEVKDDSKPKEEVKSNKNSGKESSQNQGKKSNNNKTIYLYETKIPDGKTAGDNAILIKDKNVIEKLKSAAKEENKEQTAEATKAAITSNKAKSTKKESSKVIGYTTTDSVREDGKNIFAIDRVNGENYDRKIIVGAKAETLNQSSTLQSEEAIVLPAPGKYLNGDPKKVTITQGPNIIGIHANEKENAETQKFVDWFLNSPQTWEKQSRDKKGSSEKQTAAEFFAESASYILPLKEIFDKNDTKTEKGKNSKTQQRTNTYAEKALELFKQISQNQIVSYSDPSDFRSGKFRDAIGATFNAAVSSKADFNKFVQNFTATLGSDI" }, "paralog": false, "identity": 0.9495327102803738, "score": 981.0, "evalue": 0.0, "description": "Internal stop codon at 610371, 610440, 611565, 611589. Nonsense mutation around 610371, 610440, 611565, 611589.", "psc": { "uniref90_id": "UniRef90_P75280", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75280", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "CNOCLNGIAL_1090", "locus": "GCA000143945_01086" }, { "type": "cds", "sequence": "contig_1", "start": 611772, "stop": 611951, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A45C19C" ], "nt": "TTGGAACTCTTCAAACAAATTAGTCAAAATCAAATAGTAAGCTACAGTGATCCAAGTGACTTTAGGAGTGGAAAATTCCGTGATGCAATTGGTGCTACTTTCAATGCTGCAGTTAGTTCCAAAGCAGACTTCAATAAATTCGTCCAAAACTTTACCGCTACATTAGGTAGTGACATTTAA", "aa": "MELFKQISQNQIVSYSDPSDFRSGKFRDAIGATFNAAVSSKADFNKFVQNFTATLGSDI", "aa_hexdigest": "5c0aacd6d3654e50d73802bf158724bc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A45C19C", "query_cov": 0.9152542372881356, "subject_cov": 0.9473684210526315, "identity": 0.815, "score": 92.4, "evalue": 1.1e-23, "valid": false, "product": "Putative mycoplasma lipoprotein, C-terminal region", "uniref50_id": "UniRef50_UPI000A45C19C" }, "pscc": { "uniref50_id": "UniRef50_UPI000A45C19C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A45C19C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6512.145100000002, "isoelectric_point": 5.987158012390137 }, "id": "CNOCLNGIAL_1091", "locus": "GCA000143945_01087" }, { "type": "cds", "sequence": "contig_1", "start": 612071, "stop": 612250, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGCAAATTAGGACTTACAAAATCAAAGATATTTGCGACATTCAACGTGGTCGTGGTATCACTAAGGAATACATTAAAAATAACTCTGGAAAATATCCTGTTTATTCTGCCGCTACTACCAATAACGGTGAGTTAGGTTTTATTAATACTTACGATTTTGCTGGTGAATATGTTACCTGA", "aa": "MQIRTYKIKDICDIQRGRGITKEYIKNNSGKYPVYSAATTNNGELGFINTYDFAGEYVT", "aa_hexdigest": "c88d0c6f6d89cfd8664377c21c3e12f3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 59, "aa_cov": 0.9322033898305084, "hmm_cov": 0.9830508474576272, "evalue": 3.543920618226019e-12, "score": 42.29743957519531, "start": 5, "stop": 59 } ], "seq_stats": { "molecular_weight": 6731.4927000000025, "isoelectric_point": 8.786992454528807 }, "id": "CNOCLNGIAL_1092", "locus": "GCA000143945_01088" }, { "type": "cds", "sequence": "contig_1", "start": 612434, "stop": 613072, "strand": "+", "frame": 2, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF507P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75279", "UniRef:UniRef90_P75279" ], "nt": "GTGGTAGCTGAAATCTCCTTAGATTTTCCACCTCTAGAAGTTCAAGAAAAAATTGCTCATTTTCTCAAATCTTTTAATGAATTAAGCAGCCAGTTAAAAGCTGAATTAATTAAACGACAAAAGCAATACGCATTTTACAGTGATTATCTACTCAATCCTAAACATTCACAAGGTGAAGAATACAAACTCTTTAAACTCAAAGATATAGCTAAAAAGATTTTAGTTGGCGGTGAAAAGCCAAGTGATTTCCAAAAAGAAAAGGATCAAGTTTATAAATACCCTATTCTTTCTAATAGTCGCAAAGCAGACGATTTTTTAGGCTACAGCAAAACGTTTAGAATTGCTGAAAAATCAATTACAGTTAGTGCAAGAGGCACAATTGGTGCTGTTTTTTACCGTGATTTTTCCTACTTACCCGCTGTTAGTCTAATTTGCTTTATTCCTAAGCCAGAATTTAATATTAAGTTTTTGTTTCATGCTTTAAAAGCGACTAAATTCCACAAACAAGGTTCTGGCACAGGACAGTTAACCATGGCACAGTTTAAAGAATATCAGGTTTATATTCCATCTTTAAAAAAACAACAAGAAATTGCTGCCACTTTGGATCCACTTTACTATATCTTTGCAAATTCTAATTGA", "aa": "MVAEISLDFPPLEVQEKIAHFLKSFNELSSQLKAELIKRQKQYAFYSDYLLNPKHSQGEEYKLFKLKDIAKKILVGGEKPSDFQKEKDQVYKYPILSNSRKADDFLGYSKTFRIAEKSITVSARGTIGAVFYRDFSYLPAVSLICFIPKPEFNIKFLFHALKATKFHKQGSGTGQLTMAQFKEYQVYIPSLKKQQEIAATLDPLYYIFANSN", "aa_hexdigest": "5d4b5140063308675344e44d8f8391b1", "start_type": "GTG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 612134, "stop": 613162, "observations": { "mutation": [ 612248, 613070, 613139 ], "stop": [ 612248, 613070, 613139 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75279", "query_cov": 1.0, "subject_cov": 0.5840220385674931, "identity": 0.991, "score": 412.0, "evalue": 4.94e-143, "gene_start": 1, "gene_end": 212, "reference_start": 122, "reference_end": 333, "reference_sequence": "MQIRTYKIKDICDIQRGRGITKEYIKNNSGKYPVYSAATTNNGELGFINTYDFAGEYVTWTTNGYAGVVFYRNGKFSASQDCGVLKVRNKEINAQFLAFALSLKTPQFVHNLGSRPKLNRKVVAEISLDFPPLEVQEKIAHFLKSFNELSSQLKAELIKRQKQYAFYSDYLLNPKHSQGEEYKLFKLKDIAKKILVGGEKPSDFQKEKDQVYKYPILSNSRKADDFLGYSKTFRIAEKSITVSARGTIGAVFYRDFSYLPAVSLICFIPKPEFNINFLFHALKATKFHKQGSGTGQLTMAQFKEYQVYIPSLKKQQEIAATLDPLYYIFANSNWGIYKEIELRKKQMQYYQERLFQWIENQKV" }, "paralog": false, "identity": 0.9853801169590644, "score": 669.0, "evalue": 7.26e-247, "description": "Internal stop codon at 612248, 613070, 613139. Nonsense mutation around 612248, 613070, 613139.", "psc": { "uniref90_id": "UniRef90_P75279", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF507P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75279", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75279", "EC:3.1.21.3", "UniRef:UniRef50_P75279", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75279", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75279" ], "product": "Putative type I specificity subunit S.MpnORF507P" } }, "id": "CNOCLNGIAL_1093", "locus": "GCA000143945_01089" }, { "type": "cds", "sequence": "contig_1", "start": 613190, "stop": 613456, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAACTTAAAGTTCATTGTTGATGGATCAATTCCAGTTTACACCGTTGCTAAAAAAGACTATCCTGGTTCTCTCTTCCAGTTTAATGACAAGGATGAATTGTTATTTACATTGTATGTCAAAGAACAGATTTCATTTGCCGATCCAAACTTTATGAATTTATTGAGAGGACAAAACTTGCATGATTTAGAACTAGTCACCGGAGCTACCAAACCACCGGTAGTTGATCTAGCCAGCTATTTACACTTTGTTCTTTTAAGCGCTTAA", "aa": "MNLKFIVDGSIPVYTVAKKDYPGSLFQFNDKDELLFTLYVKEQISFADPNFMNLLRGQNLHDLELVTGATKPPVVDLASYLHFVLLSA", "aa_hexdigest": "c229286079eed9429ab7835a6239f359", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 47, "aa_cov": 0.5340909090909091, "hmm_cov": 1.0, "evalue": 4.1177268891677024e-17, "score": 58.00649642944336, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 9936.369199999992, "isoelectric_point": 5.066252708435058 }, "id": "CNOCLNGIAL_1094", "locus": "GCA000143945_01090" }, { "type": "cds", "sequence": "contig_1", "start": 613700, "stop": 613873, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCAGATAGAAACAGAACTTTCAGTTTCTTAATTGCTGGATTTCGAACTGCCATTGATTGGTATTACAACGCTAAAAAAGAAAATAACGATGCGAAGCAAAAGGCCTTTGGTAGTCAAGGAATCCAATTCCCTAAAGATGGTTTAAATGGAATTTACATGTCCGATTGA", "aa": "MEADRNRTFSFLIAGFRTAIDWYYNAKKENNDAKQKAFGSQGIQFPKDGLNGIYMSD", "aa_hexdigest": "dfe380c494a1cd9798e2d5462de4b0aa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6541.214400000003, "isoelectric_point": 8.034259605407716 }, "id": "CNOCLNGIAL_1095", "locus": "GCA000143945_01091" }, { "type": "cds", "sequence": "contig_1", "start": 613934, "stop": 614281, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATAAGCTAATTTCAAACGGCACAAATGATTTAACTACACTTGCTCAAACTGCTTTAGGTGATTCTTTATTTGATACTAAAGTTAACCTGACAGAATCAGTTATTGAAATAAATAACCAAACAGATGTTGCAACTAAATTTAGGGAAAAAGTACTCAATCCTTTCAAGCAAGAAAGAGAAAAAGCACATGCTGAGCACGTTGAAAAGTTAAGGAAACTAGAAGAGGAGCGTAAACTTCAAGAAGCAGAGGCCAAAGCAAAAGCAGAGGAAGTTAAAAAACTCGAAGCAGAAAGAGAAGCTTTTAACAAATCTCTAACAGCTGCATCAGAATTCAAACAATATTGA", "aa": "MHKLISNGTNDLTTLAQTALGDSLFDTKVNLTESVIEINNQTDVATKFREKVLNPFKQEREKAHAEHVEKLRKLEEERKLQEAEAKAKAEEVKKLEAEREAFNKSLTAASEFKQY", "aa_hexdigest": "feae9dad62df26e7bbb04880e7674d64", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13159.652299999987, "isoelectric_point": 5.972948265075684 }, "id": "CNOCLNGIAL_1096", "locus": "GCA000143945_01092" }, { "type": "cds", "sequence": "contig_1", "start": 614327, "stop": 614740, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "GTGCAAAACACGGTTAATTTAATGAATCAACTTCAAGCAGAAATAAATGCAGCTAATGCTGTGTTTCCTGTTTCCGACAGCACTAAAATTCCTAAAGTGTCACAAAAGCTTTTTGGTTTACTTGGTGATGGTTTCTTTCCGCAATTACATCCAAAGGGTTTAAAGATTGCTGACAATATTGCTGCATTATTCGATCAATATAACCTTAAGTCGATTGCACTCAAAAACTTTGATTTAAATTTAGAGCGAAAAAACGACATAGTCATTCAGGGCAAAGTTTGTTATAGTTTTTCCATCCAAATGGATTTTGCAACTATTTATGAAGGTGATGGTAGCACGATAGATTTACAGTTTGCACTCAACGCATCCACTACTAATTTTGCCAATCTAACAGATTTACAAGACAGTTTCTGA", "aa": "MQNTVNLMNQLQAEINAANAVFPVSDSTKIPKVSQKLFGLLGDGFFPQLHPKGLKIADNIAALFDQYNLKSIALKNFDLNLERKNDIVIQGKVCYSFSIQMDFATIYEGDGSTIDLQFALNASTTNFANLTDLQDSF", "aa_hexdigest": "09f380e0abb91fb5e49fefba57440514", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 140, "aa_cov": 0.9927007299270073, "hmm_cov": 1.0, "evalue": 2.4432068832046932e-45, "score": 150.159912109375, "start": 2, "stop": 137 } ], "seq_stats": { "molecular_weight": 15157.043799999981, "isoelectric_point": 4.718454933166503 }, "id": "CNOCLNGIAL_1097", "locus": "GCA000143945_01093" }, { "type": "cds", "sequence": "contig_1", "start": 614849, "stop": 615100, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGCAACTAAAAAAATTCGCTTCAAAACAAAAGCGTTTTTGCTGGCTTTTTGTACCACTACTGTAACTTTAGGCGCATTTTTATTTCCTACATTAACTCAAAAAGGTTCTAGTGCTGACATTAATACTTTAGCACTACACAATAAAAGTTTAGCTAGAGCCCATAATGACAGCTACTTGGTCAATCTTGCTAACGCATCTACTTTTAGTGAGGAAGAGTTAGCTGTAATAGCGAAAAATAAGGATTGA", "aa": "MKATKKIRFKTKAFLLAFCTTTVTLGAFLFPTLTQKGSSADINTLALHNKSLARAHNDSYLVNLANASTFSEEELAVIAKNKD", "aa_hexdigest": "ca99b181c10a4704c824a4da2ae646e7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9063.374699999995, "isoelectric_point": 9.666728019714355 }, "id": "CNOCLNGIAL_1098", "locus": "GCA000143945_01094" }, { "type": "cds", "sequence": "contig_1", "start": 616401, "stop": 616664, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "TTGTTTAATGACTACAACTTAAATTCAATTAAGTTAAAAGCGTTTAACTTGCAGTTGCTCAAACAAAACGACATTGTTTTAAAGAACAAGGTGCGCTATAGTTTTGCCATCGAAATGAAATTTCATACCGTTTATGTTGGCAAAAATACCAAAATTGACCTTGATTTTGCTCTGCAAGCTCAAACGAATAACTTTAGCTCTTTAGAGGAATTACGTGAAAGTTTTACTAATAGTGGTCAAACACTCTCAACCCAATTGTTCTGA", "aa": "MFNDYNLNSIKLKAFNLQLLKQNDIVLKNKVRYSFAIEMKFHTVYVGKNTKIDLDFALQAQTNNFSSLEELRESFTNSGQTLSTQLF", "aa_hexdigest": "252eb302644e232664999629963a48f0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 87, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.9517053340798807e-29, "score": 98.10533142089844, "start": 1, "stop": 87 } ], "seq_stats": { "molecular_weight": 10130.437499999993, "isoelectric_point": 9.004250907897951 }, "id": "CNOCLNGIAL_1099", "locus": "GCA000143945_01095" }, { "type": "cds", "sequence": "contig_1", "start": 617527, "stop": 617769, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "TTGGCTAACTGGGGTCTTGGTGGCATTCCGCCAGAATGGAGTGGTGACATGGAATTGAAGTTCCAAATTGATGGTAAGCTAGCTAACTGGATTACCCAAAAGCAAGATTACCCTGGTTCGTTATTCCAATTTCAAAATGACAAACTGTTGTTTACCCTACACGTAGTGCAACGTATTACCGTTAAGGATCAACGTTTCATGAACCTGCTAAAGAAACAGCAATTGGATGTGATTGAACCTTGA", "aa": "MANWGLGGIPPEWSGDMELKFQIDGKLANWITQKQDYPGSLFQFQNDKLLFTLHVVQRITVKDQRFMNLLKKQQLDVIEP", "aa_hexdigest": "16af518b765bf6177910c10238d12513", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 59, "aa_cov": 0.725, "hmm_cov": 1.0, "evalue": 2.2892042364601864e-27, "score": 91.21879577636719, "start": 6, "stop": 63 } ], "seq_stats": { "molecular_weight": 9347.710299999997, "isoelectric_point": 6.54025821685791 }, "id": "CNOCLNGIAL_1100", "locus": "GCA000143945_01096" }, { "type": "cds", "sequence": "contig_1", "start": 618277, "stop": 618579, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "GTGGTAGAACAATTAACCACAGATGGTGGCAATGATCTTACCAACATTGCTAAAACAGCAATTGGTGAAAGTTTGTTTAACCTCAAGGTTAACTTAACTGATTCAATAATTGATCAAAGCGTTTTACAGCAAGCGCAAAAATCATTTGAGGACAAGATCTTAGATCCGTTCCACGCTGAACGAGTTGAAGCTAAGCGCATTCATGATGAAGAAGCAAGACGCTTAGAGGCGGAACGAAAACGGATTGCAGCTGAATTGAAAGCCAAGGAAGATGAAGCTCGTCGTATTCGTGAAGAACATTGA", "aa": "MVEQLTTDGGNDLTNIAKTAIGESLFNLKVNLTDSIIDQSVLQQAQKSFEDKILDPFHAERVEAKRIHDEEARRLEAERKRIAAELKAKEDEARRIREEH", "aa_hexdigest": "b54a6cae6e0e8032f9d0c95e6e56fd6f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 36, "aa_cov": 0.36, "hmm_cov": 0.9722222222222222, "evalue": 2.9215895231398272e-08, "score": 28.79109764099121, "start": 2, "stop": 37 } ], "seq_stats": { "molecular_weight": 11482.709799999988, "isoelectric_point": 5.236599159240721 }, "id": "CNOCLNGIAL_1101", "locus": "GCA000143945_01097" }, { "type": "cds", "sequence": "contig_1", "start": 618586, "stop": 618954, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGCTTACTGTCCCTAAAGTGCCGGAAAACTTCTTTAGTTTCTTTCAGGAAGATTACTTTCCGCGGTTAACTCCCCGTGGTTTGAACATTGCCGATAATGTGGCTTCCTTGTTTAATGACTACAACTTAAATTCAATTGACTTTACTGGTTTTAACCTAAAACTGTTGCGCCAAAACGACATTGTTTTAAAGAATAAGGTGCGTTACAACTTCGCTCTTCAAATGGGCTTTGACACGGTTTACGCTGGAAAGAACACCACTATTTTAAAGTTTAGTTTACAAGCCCAAACAATAAACTTCACTAGCTTACAAGAACTACGTGATAGTTTTGAAAACAATGGCAACACTCTTTCAACCCATCTATTCTGA", "aa": "MLTVPKVPENFFSFFQEDYFPRLTPRGLNIADNVASLFNDYNLNSIDFTGFNLKLLRQNDIVLKNKVRYNFALQMGFDTVYAGKNTTILKFSLQAQTINFTSLQELRDSFENNGNTLSTHLF", "aa_hexdigest": "ba99fc209b20e23f6fffaba29767945e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 120, "aa_cov": 0.9754098360655737, "hmm_cov": 1.0, "evalue": 3.260611702653205e-36, "score": 120.28932189941406, "start": 4, "stop": 122 } ], "seq_stats": { "molecular_weight": 14103.79939999998, "isoelectric_point": 6.551228141784668 }, "id": "CNOCLNGIAL_1102", "locus": "GCA000143945_01098" }, { "type": "cds", "sequence": "contig_1", "start": 619142, "stop": 619303, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTACCCACTTTTTCCCAAAATGGCTCAGCTACTGATCCACAAACTGTAAGTTTAAACAGCAGTAGCTTTGAAAAGCATAACAGTAAGAAATTCTTATTGAAGCGCAAAACTGCTTTAAGTAACGCTGAGTATGAAAACCTTTCCAAGCAAACTAACTGA", "aa": "MVPTFSQNGSATDPQTVSLNSSSFEKHNSKKFLLKRKTALSNAEYENLSKQTN", "aa_hexdigest": "09efebef237f494ae6594bddeff84250", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5905.4779, "isoelectric_point": 9.601679420471193 }, "id": "CNOCLNGIAL_1103", "locus": "GCA000143945_01099" }, { "type": "cds", "sequence": "contig_1", "start": 619567, "stop": 619707, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAGCCAGAATTCATGGAATTAATTAAGTCACAAAACCTCCATAACTTAGAACTACGTACCGAGTCCACAAAGCCGCCAGTAGTGGATTTAGCGAGTTATTTACACTACCAACTGTTGAAGTTAAATCAACAGTAA", "aa": "MQEPEFMELIKSQNLHNLELRTESTKPPVVDLASYLHYQLLKLNQQ", "aa_hexdigest": "7f344c8e8368acec30a9529943802a50", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5468.2211, "isoelectric_point": 5.461397361755371 }, "id": "CNOCLNGIAL_1104", "locus": "GCA000143945_01100" }, { "type": "cds", "sequence": "contig_1", "start": 619900, "stop": 620349, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_511", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75275", "UniRef:UniRef90_P75275" ], "nt": "GTGCAAGCCTTTAAAGAATACTGGCAAAAGCAAAAGAAAGATGTTACCGATAAAAAGCAGCTGTTAGAAGCTTTAAAACTTTCCTTTGCCAAAGAACAAAATAAGACCTTTGCCTTTTTGATTAAGAACTTCCAGGACGGTATTAGCAATTATTACCCAAATGACCAAGAAGATCAAAGTGAGGCTGCTAAAACTGCTTTTGGTACACAAGGTATCGCCTTTCCCCAATCTGGTTTAAAGGGCATCTTTATGTCGGAATGGTTGCGTAAACAGCTAGGGGAAAAGGCCAAGATTAACTTAGACATAAAGAGCTTAAAGGTAACTGATTCAAAGATAAGTCCTACCATTAAATGGAACAAAGATATCGGTATTAAGCGAAATCAAGACAAACCTTACAACTTCCGCTTTGAGATTGACATTGAATATCAAGGTAATTACAAATTAAGTTGA", "aa": "MQAFKEYWQKQKKDVTDKKQLLEALKLSFAKEQNKTFAFLIKNFQDGISNYYPNDQEDQSEAAKTAFGTQGIAFPQSGLKGIFMSEWLRKQLGEKAKINLDIKSLKVTDSKISPTIKWNKDIGIKRNQDKPYNFRFEIDIEYQGNYKLS", "aa_hexdigest": "551226bacf23f303f751412bc9ba648a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 619597, "stop": 620349, "observations": { "mutation": [ 619722, 619809, 619857, 619902 ], "stop": [ 619722, 619809, 619857, 619902 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75275", "query_cov": 1.0, "subject_cov": 0.573076923076923, "identity": 1.0, "score": 297.0, "evalue": 2.44e-100, "gene_start": 1, "gene_end": 149, "reference_start": 1, "reference_end": 149, "reference_sequence": "MQAFKEYWQKQKKDVTDKKQLLEALKLSFAKEQNKTFAFLIKNFQDGISNYYPNDQEDQSEAAKTAFGTQGIAFPQSGLKGIFMSEWLRKQLGEKAKINLDIKSLKVTDSKISPTIKWNKDIGIKRNQDKPYNFRFEIDIEYQGNYKLSWLEAIIAKFSGIPGEWKGKLNLKFIVDGDLSWEIVQKPDYPGSLFQFDDQKQQLLFKLHVWEKITVQEPEFMELIKSQNLHNLELRTESTKPPVVDLASYLHYQLLKLNQQ" }, "paralog": false, "identity": 0.98, "score": 485.0, "evalue": 8.63e-177, "description": "Internal stop codon at 619722, 619809, 619857, 619902. Nonsense mutation around 619722, 619809, 619857, 619902.", "psc": { "uniref90_id": "UniRef90_P75275", "product": "Uncharacterized protein MPN_511", "uniref50_id": "UniRef50_P75275", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75275", "UniRef:UniRef50_P75275" ] }, "pscc": { "uniref50_id": "UniRef50_P75275", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75275" ], "product": "Uncharacterized protein MPN_511" } }, "id": "CNOCLNGIAL_1105", "locus": "GCA000143945_01101" }, { "type": "cds", "sequence": "contig_1", "start": 620380, "stop": 620649, "strand": "-", "frame": 3, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029624419", "UniRef:UniRef90_UPI0029624419" ], "nt": "GTGTTGGGTAATAACAATGATTTAACAACTGTAGCAAAAGCAGCTGTTGGTGATAATTTATTTACCACCCAAGCTAATTTAACCAAGTCAGTTTTGGATCAAACCGTGCTAAAAGAGGCTGAATCAAGGTTTGAAGCAACTGTTTTAAAACCGTTCATTGAAGCACGGCAAAAGGCACTAGCGGAACACCAAGCACACCAAAAAGTCTTAGAGGAGCAACGCCAAAAACAGCTCGAAGAATTAAAACAAAAACAAAAGGAAGCTGAATAG", "aa": "MLGNNNDLTTVAKAAVGDNLFTTQANLTKSVLDQTVLKEAESRFEATVLKPFIEARQKALAEHQAHQKVLEEQRQKQLEELKQKQKEAE", "aa_hexdigest": "15ef2cc78a3a3556ba18ef463dc36d14", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 620431, "stop": 620724, "observations": { "mutation": [ 620673 ], "stop": [ 620673 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029624419", "query_cov": 1.0, "subject_cov": 0.7807017543859649, "identity": 0.9890000000000001, "score": 136.0, "evalue": 7.91e-40, "gene_start": 1, "gene_end": 89, "reference_start": 26, "reference_end": 114, "reference_sequence": "MQDSLVLRGQNLTTQLFWKPTVKRLVLGNNNDLTTVAKAAVGDNLFTTQANLTKSVLDQTVLKEAESRFEATVLKPFIEARQKALAEHQAHQKVLEEQRQKQLEELKQKQKEAE" }, "paralog": false, "identity": 0.979381443298969, "score": 182.0, "evalue": 1.1e-60, "description": "Internal stop codon at 620673. Nonsense mutation around 620673.", "psc": { "uniref90_id": "UniRef90_UPI0029624419", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI0029624419", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029624419", "UniRef:UniRef50_UPI0029624419" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029624419", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029624419" ], "product": "DUF240 domain-containing protein" } }, "id": "CNOCLNGIAL_1106", "locus": "GCA000143945_01102" }, { "type": "cds", "sequence": "contig_1", "start": 620818, "stop": 621210, "strand": "-", "frame": 3, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4F51D6", "UniRef:UniRef90_UPI000A4F51D6" ], "nt": "TTGCAAACTAAGTTTAAGGACAATTCGCTTAACCCACAACAGTTGGGTGTGATTGATGTTTACAACCTGTTGAGTGGATTGGAACAAAGTACGCGTGATACCGTTAAGTTAATTAATGACTTAAATGCAAAGGTATTACTAGCAAATGCCGTTTTTCCGGTGCACGAGGCATTTCAATTGCCCCAAGTACCCGAACAGCTGTTTACCATTTTGGGTAGTGATTTCTTTCCTCAATTAACCCCTAAGGGTTTAAATGTGACTGAGAATATTACGACAGTTTTGCAACAATACAAACTTCAAAGCATTGCGTTAAAGCAGTTTGATCTGAAACTAGAAAAACAACCGGACATTGTGAAGCGCTACAAGATGCGTTACAGCTTCGTAATCCAATGA", "aa": "MQTKFKDNSLNPQQLGVIDVYNLLSGLEQSTRDTVKLINDLNAKVLLANAVFPVHEAFQLPQVPEQLFTILGSDFFPQLTPKGLNVTENITTVLQQYKLQSIALKQFDLKLEKQPDIVKRYKMRYSFVIQ", "aa_hexdigest": "02e1bd1b17cff7fe0eb095975645971f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 620639, "stop": 621471, "observations": { "deletion": [ 620733 ], "mutation": [ 620820, 621231, 621384 ], "stop": [ 620820, 621231, 621384 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A4F51D6", "query_cov": 1.0, "subject_cov": 0.4693140794223827, "identity": 0.992, "score": 252.0, "evalue": 8.54e-83, "gene_start": 1, "gene_end": 130, "reference_start": 88, "reference_end": 217, "reference_sequence": "MKLIKKKRLLLKTLSLSLAGLTTTLAIVVWPTLTEKTNQSVSDFTLSNQRFEQNQSNRYQLQFNSGISQADLDYTTKHTNWKANFEQLQTKFKDNSLNPQQLGVIDVYNLLSGLEQSTRDTVKLINDLNAKVLLANAVFPVHEAFQLPQVPEQLFTILGSDFFPQLTPKGLNVTENITTVLQQYKLQSIALKQFDLKLEKQPDIVKRYKMRYSFVIQWDFETVYTGVNSTTNLAFSVKAMTTNFANLTGIARQLGFTWPELNYTTFLKANGQAVSVG" }, "paralog": false, "identity": 0.9855595667870036, "score": 523.0, "evalue": 9.08e-192, "description": "Internal stop codon at 620820, 621231, 621384. Frameshift due to deletion around 620733. Nonsense mutation around 620820, 621231, 621384.", "psc": { "uniref90_id": "UniRef90_UPI000A4F51D6", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000A4F51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A4F51D6", "UniRef:UniRef50_UPI000A4F51D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A4F51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4F51D6" ], "product": "DUF240 domain-containing protein" } }, "id": "CNOCLNGIAL_1107", "locus": "GCA000143945_01103" }, { "type": "cds", "sequence": "contig_1", "start": 621622, "stop": 622074, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_513", "db_xrefs": [ "RefSeq:WP_010874869.1", "SO:0001217", "UniParc:UPI000013ACFB", "UniRef:UniRef100_P75273", "UniRef:UniRef50_P75273", "UniRef:UniRef90_P75273" ], "nt": "ATGTACGACATGATTAATGCCAAGTACTTGGGCAAGTTAATCTTTTTGGATTTTGGCCAAACAATTACGAGTTTGGAAGAAACGAGTGATAACTCGACCTTTTTTATTGCTGATGAATTTGGTAATTTTGGGAGCCACTATGTGGGGGTGGTATTGGAGCAAGTCCGCTCGTTCAATACTGTAGTTATCCTGTCCTATCAATCATTTGCTAACCTGCGCAATATTGGTGGTGATAACTTTAAATCACAGATTATTAACAACACCAGTACCCTAATTGCCCACCGCTTAATTGACATGATGGAAGCAGAAGAAGTGGCTAACCTGTATGGGGTTGATATTACCTGTGCTAGTGAAATTAAAAGTTTAAAAGTGGGTCAAGCACTAGTGCGCAGTATCTCTAGTTATTATGGCGAAATCAAAGACTGGCTGGTGCAACAAATCGATAAAAGCTAA", "aa": "MYDMINAKYLGKLIFLDFGQTITSLEETSDNSTFFIADEFGNFGSHYVGVVLEQVRSFNTVVILSYQSFANLRNIGGDNFKSQIINNTSTLIAHRLIDMMEAEEVANLYGVDITCASEIKSLKVGQALVRSISSYYGEIKDWLVQQIDKS", "aa_hexdigest": "cb8379474cbdfd0cfb5bb4e6f49746f2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ACFB", "ncbi_nrp_id": "WP_010874869.1", "uniref100_id": "UniRef100_P75273", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ACFB", "RefSeq:WP_010874869.1", "UniRef:UniRef100_P75273" ] }, "ips": { "uniref100_id": "UniRef100_P75273", "uniref90_id": "UniRef90_P75273" }, "psc": { "uniref90_id": "UniRef90_P75273", "product": "Uncharacterized protein MPN_513", "uniref50_id": "UniRef50_P75273" }, "pscc": { "uniref50_id": "UniRef50_P75273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75273" ], "product": "Uncharacterized protein MPN_513" }, "genes": [], "id": "CNOCLNGIAL_1108", "locus": "GCA000143945_01104" }, { "type": "cds", "sequence": "contig_1", "start": 622079, "stop": 622342, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014575013.1", "SO:0001217", "UniParc:UPI0001DD6414", "UniRef:UniRef100_A0A0H3DLB1", "UniRef:UniRef50_A0A0H3DLB1", "UniRef:UniRef90_A0A0H3DLB1" ], "nt": "TTGTTTAAAAAAATTCACAAAGAAAAACGCCGAAAAGAAATTTTAGAAATGAAAGAAAAAGGTCGCTTTCAACCTTCCAATCAAATTTTTGTAGAAAAAATTGATGAATACTATACGGAGCATGTTCAAACCATTGACACAAAGGACTTTGAAAATTTAAGAGAAAACCTCAGGGAACTAGCGGAAATTGGTTCAATCGATTCTAATGGTGTGGGATTGATAGATATTATCAAACTGCCAAGATGCTGTTTTGTTTTCCATTAG", "aa": "MFKKIHKEKRRKEILEMKEKGRFQPSNQIFVEKIDEYYTEHVQTIDTKDFENLRENLRELAEIGSIDSNGVGLIDIIKLPRCCFVFH", "aa_hexdigest": "d4a9f5f12a5637ee2633483fdccff83a", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6414", "ncbi_nrp_id": "WP_014575013.1", "uniref100_id": "UniRef100_A0A0H3DLB1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6414", "RefSeq:WP_014575013.1", "UniRef:UniRef100_A0A0H3DLB1" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DLB1", "uniref90_id": "UniRef90_A0A0H3DLB1" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DLB1", "uniref50_id": "UniRef50_A0A0H3DLB1" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DLB1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DLB1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10427.929099999992, "isoelectric_point": 6.830591773986817 }, "id": "CNOCLNGIAL_1109", "locus": "GCA000143945_01105" }, { "type": "cds", "sequence": "contig_1", "start": 622591, "stop": 622953, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_514", "db_xrefs": [ "RefSeq:WP_014575015.1", "SO:0001217", "UniParc:UPI0001DD6416", "UniRef:UniRef100_A0AAV5N8F8", "UniRef:UniRef50_P75272", "UniRef:UniRef90_P75272" ], "nt": "ATGGAACAGGACATCAATAACCAGACTGGTAAGAAAATCTTTTTAAACGAGGAGTGTTTTTTGGAACTGGAAAAGCTCCCCCAGCACGTCTCGGTACTAGGTGTGAGTGGTTTTGGTAAGTCCAATATCTTGCTGCACTTTTTGAAGTATGCAATTGACAACGACCATCCCTTAATTTTTGTGAATGGTAAGGGTGATAAGGAATTAATTACCCAGTTTGAACACTACCAAACGAGCGCCAAACAACTAAGTCCCGAAGACGGTTTTGATACGGTTAAGTTAGTGGCACTCAAAAATACTAGTGCCAAAATTTGGAGCTTAGATGACAGAATTGCCACGATTAAGTACAACCCGTTTAAGTAA", "aa": "MEQDINNQTGKKIFLNEECFLELEKLPQHVSVLGVSGFGKSNILLHFLKYAIDNDHPLIFVNGKGDKELITQFEHYQTSAKQLSPEDGFDTVKLVALKNTSAKIWSLDDRIATIKYNPFK", "aa_hexdigest": "bff15c97f09093293f8856bd7136ebed", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6416", "ncbi_nrp_id": "WP_014575015.1", "uniref100_id": "UniRef100_A0AAV5N8F8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6416", "RefSeq:WP_014575015.1", "UniRef:UniRef100_A0AAV5N8F8" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N8F8", "uniref90_id": "UniRef90_P75272" }, "psc": { "uniref90_id": "UniRef90_P75272", "product": "Uncharacterized protein MPN_514", "uniref50_id": "UniRef50_P75272" }, "pscc": { "uniref50_id": "UniRef50_P75272", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75272" ], "product": "Uncharacterized protein MPN_514" }, "genes": [], "id": "CNOCLNGIAL_1110", "locus": "GCA000143945_01106" }, { "type": "cds", "sequence": "contig_1", "start": 623115, "stop": 624638, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "GTGAAGGAAAAGGTGTCGAGTGAGATCCAGGACTTAATTAAACGTGAAGAGTACCGTGATAACTCGATTGTTGTCATGGCCGATTCCGGGGCGCGGGGTAATATCTCCAACTTTACCCAGCTGTTTGGGATGCGGGGGTTAATGAGTAAGAGCTTTAACTATGAACGCAACAACCAGTCCAAAATTATTAAGGACACGATTGAGGTTCCTATTAAGCACTCCTTCCTGGAAGGTTTAACGATTAACGAGTACTTCAACTCATCTTATGGGGCGCGTAAGGGGATGACCGATACCGCCATGAAAACGGCTAAGTCTGGTTACATGACCCGTAAACTGGTGGATGCAACCCACGAATTGATTATTAACCACGATGAATGTGGTACACGCAAGGGAATTGTGGTCGAAGCAATTGTGGAAACCAAAACCCGTTCGTTGGTGGAATCACTGTTTGACCGCATTGTCAACCGTTACACGATTGGACCAATTTTAGATCCCGAAACCAAGGCGGAAATTGTCCCTGCTAACAGCCTAATTACCCAAGAGTTAGCCAAGCAAATTTGTGCCACTTCGATTAAACAAGTGTTAGTGCGCTCTGTGATTTACTGTGAACGCGAAAACGGCGTGTGTCAGTACTGTTTCGGGGTCGATTTGTCCACCGGCAAACTGGTGGAATTGGGTACCGCTGTGGGTGTAATTGCAGCGCAATCGATCGGTGAACCAGGAACCCAGTTAACGATGCGTACCTTCCACACCGGGGGGGTTTCGACCGAAAACAACTTGGCCCAAGGGTTTGAACGTTTAAAGCAAATCTTTGAAGTAGTCGCCCCTAAGGATTACGAGCGCTGTGTCATTTCTGAAGTTAAGGGGGTAGTTAAATCGATTACTACCACCCAAAACGCGCAGGAAGTGTTGATTGAATCTAGTGTCGATGAACGCACTTACTCGATTCCGTTTAGTGCGCAACTGCGTGTCAAGGTCGGTGATGCAGTAGAACTAGGTTCGAAGATTACCGAAGGTTCGATTGACATTCGCCAGTTATTACGGGTTGCTGGGATCCAACGGGTACGCCAGTACATGATTGTCGAAATCCAAAAGGTGTACCGTATTCAGGGGATTGAGATTGCTGATAAGTATGTCGAAATTATTATCCGCCAGTTGACGAGCTTACTCCAAGTAACCGATGCTGGCAGCAGTAACCTGTTTGTGGGTCAACTGGTACACAGTCACCACCTCAATGAACTCAACAAGAGCTTGTTGCTGTCGGGGAAGATGCCAGTGATTGCCATTAACCAGGTGTTTGGGATCGATGAGGCGGCCAGCAAGTCGAACTCCTTCTTGAGTGCCGCATCCTTCCAAGACACCAAGAAGATCTTGACCGATGCTGCGGTAAAAACCCAAGTTGACTACCTCTTAGGGTTGAAAGAAAACGTGATCATTGGGGGTAAGATCCCGGCTGGTACTGGGTTTTTAACCGATGAAGAATTGGCCTACCTCGGTGCCAAAACGGTTCAAGAGGAGTATTAA", "aa": "MKEKVSSEIQDLIKREEYRDNSIVVMADSGARGNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFLEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDECGTRKGIVVEAIVETKTRSLVESLFDRIVNRYTIGPILDPETKAEIVPANSLITQELAKQICATSIKQVLVRSVIYCERENGVCQYCFGVDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVAPKDYERCVISEVKGVVKSITTTQNAQEVLIESSVDERTYSIPFSAQLRVKVGDAVELGSKITEGSIDIRQLLRVAGIQRVRQYMIVEIQKVYRIQGIEIADKYVEIIIRQLTSLLQVTDAGSSNLFVGQLVHSHHLNELNKSLLLSGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKTQVDYLLGLKENVIIGGKIPAGTGFLTDEELAYLGAKTVQEEY", "aa_hexdigest": "112c9511e2ee87c972bcfaf17e3b675e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 77, "aa_cov": 0.14398422090729784, "hmm_cov": 0.987012987012987, "evalue": 5.2031182544330415e-17, "score": 57.23020553588867, "start": 3, "stop": 75 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 341, "aa_cov": 0.6331360946745562, "hmm_cov": 0.7536656891495601, "evalue": 1.713997003629071e-58, "score": 193.60702514648438, "start": 78, "stop": 398 } ], "seq_stats": { "molecular_weight": 56015.58430000006, "isoelectric_point": 6.255779075622559 }, "id": "CNOCLNGIAL_1111", "locus": "GCA000143945_01107" }, { "type": "cds", "sequence": "contig_1", "start": 624645, "stop": 626828, "strand": "-", "frame": 1, "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "db_xrefs": [ "COG:COG0086", "COG:K", "EC:2.7.7.6", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270", "KEGG:K03046", "SO:0001217", "UniRef:UniRef50_P47582", "UniRef:UniRef90_P47582" ], "nt": "TTGTTTGATGAAGCCATTTTTGGTCCAGTGAAGGACTATGAGTGTGCTTGTGGGAAGTTTAAGAAGATTAAGTACCGTGGCGTGCGCTGTGATCGCTGTGGTGTTTGGGTAACCGAATCAATTGTGCGCCGGGAACGTATGGGCCACATTGCCCTAGTCAGTCCGGTAGCGCACATCTGGATGTCGAAGGAACTGCCTTCCCCTTCCAAGATCTCCTTAGTGTTAAACATCTCTTATAAGGAAGTGGAACAGGTCTTGTACTTTGTTAACTACATAGTATTAGATACTGGGAAGATCAAAGATCCCAAGATCATGCCGTTTAAGTTTAAGGAAGTGTTGGACTTAGCAGGCAAGGGTTCTTTAACCACGCGGCAAAAAATGCGCCGGGTGATCGGTTACATCTTCCGGAACCTGATTAAGAACAGGAGTAGCGAAGACTACCGCAAGGGGAAGATCTTTTATGAAAGCTTAAAGAACAGTTCGTTACCGTTCTCCTTAAATGATGCCTTTAACTACATTAAGAAGTACACTGGCTTCCGCGTTGGTATCGGGGCTGAAGCCATCTTAGAATTGCTCAACAAGATTGACCTTAACTATGAGTTTAGTAAGCTCAATGATGCGTTACGGAAGGCTAAGAAGGATAGTGTTGAGTATGCTAAGGTTAAAAAGATCTTGCGTCAATTGGAAACAATTAGCTGGTTTAGAAATTCGAAGCTGCACCCGAAAAACATGATCCTGCACACGGTGCCAGTGATCCCGCCAGACATCCGGCCGATTATCCAGTTAGATGGTGCTAAATTTACCACCAGTGACATTAATAACTTCTACAGAAGGGTAATTATCCGTAACGACCGTTTACGCCGCATCTTGGAAGATGGTACCGTACCTGCCATTGTGGTTAACAACGAAAAGCGCTTGTTACAAGAATCAGTTGATGCCTTGTTTGACAACTCGTCGCGCCACAAGCCAGCGTTATCCAAAGACAAACGTTCCTTGAAGTCACTCACCGACCGCTTAAAAGGGAAACAAGGGTTGTTCCGTCACAACCTTTTAGGTAAACGGGTGGACTATTCTGGTCGGAGTGTAATTGTGGTGGGTCCAGAACTAAAGATGTATGAGGTCGGGATTCCAGCATTAATGATCCTCAAGCTGTTCAAACCATTTATTATTCACGGGTTAATTAATAAGTTTGACAGCAACGGTAACGAAATTAGACCAATTGCGTCCTCGATTCGGCAAGCGGAGGACATGATTAAAAATCAGGACGACCTGATCTGGGGGATTGTGTATGATGTCATTAAGGACCGTCCGGTGTTATTAAACCGGGCACCTACTTTGCACCGTTTGGGAATACAGGCCTTTGAACCGCGGATTGTCGATGGGAAAGCGATTCGCCTACACCCGTTAGTGACCACTGCTTTTAACGCTGACTTTGACGGTGACCAGATGGCGGTGCACGTTCCGTTGAGCGAAAATGCCGTTAACGAAGCGCGTGCTATTCTGTTAGCTTCCAAGCACATCTTGGGACTAAAAGATGGCCGGCCAATTGTCACCCCAACCCAGGACATGGTGTTAGGTAACTATTACCTCACTACTGAGCGTAAGGGTCAAACGGGTGAAGGCATCATCTTTGGTACCGTGCATGAAGCCCGCGCGGCATACGAAGCGGGCAAGGTGCACCTCCACGCCATTGTCGGCATTAGCACCAAAGCCTTTCCAAACAAGCACTTTGAAGCTCAAGGTACTCTAATCACTACGGTTGGTAAGATTATCTTTAACGATGTGTTAGGGGACAACATCCCTTACATTAATGAAGGGGAATTTGATGAACATGCGTGTCCGCAAAAGTTCATAGTACCACCAAGTGGTGATGTGCGTGCTGCGATTGCCGCACACCAAGTGCTCCCTGCCTTTGGTAAGAAGGTCATCTCCAAGTTAATTGACTTACTTTACACGGTGGTTGAATTTAAGGATCTACCACGGATCTTGGACAACATTAAGGCCTTAGGCTTTAAGTACTCGACCCACTCTTCCACCACTGTATCGGTGTTTGATATTCCGAAGTACTCCAACAAACAGCAGTACTTTGATGAAGCGGACCAACAGGTCTTGAAGTACAAGCAGTTTTACAACAAGGGTTTACTGACCGATGATGAACGGTATAAACGGGTGGTGAAACTGTGA", "aa": "MFDEAIFGPVKDYECACGKFKKIKYRGVRCDRCGVWVTESIVRRERMGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDPKIMPFKFKEVLDLAGKGSLTTRQKMRRVIGYIFRNLIKNRSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNYEFSKLNDALRKAKKDSVEYAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPAIVVNNEKRLLQESVDALFDNSSRHKPALSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDSNGNEIRPIASSIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAILLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQTGEGIIFGTVHEARAAYEAGKVHLHAIVGISTKAFPNKHFEAQGTLITTVGKIIFNDVLGDNIPYINEGEFDEHACPQKFIVPPSGDVRAAIAAHQVLPAFGKKVISKLIDLLYTVVEFKDLPRILDNIKALGFKYSTHSSTTVSVFDIPKYSNKQQYFDEADQQVLKYKQFYNKGLLTDDERYKRVVKL", "aa_hexdigest": "f920cbe204c7872a2c2ef540f4583d2c", "start_type": "TTG", "rbs_motif": null, "genes": [ "rpoC" ], "truncated": "both", "pseudogene": { "start": 624342, "stop": 626984, "observations": { "mutation": [ 624647, 626897 ], "stop": [ 624647, 626897 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47582", "query_cov": 1.0, "subject_cov": 0.5626934984520123, "identity": 0.935, "score": 1344.0, "evalue": 0.0, "gene_start": 1, "gene_end": 727, "reference_start": 56, "reference_end": 782, "reference_sequence": "MTTTRRNKRNNKLYKNIKAIKLSIASNDTILNWSEGEVTKAETINYKSLKPEPGGLFDEAIFGPVKDYECACGKFKKIKYRGVRCDRCGVWVTESIVRRERMGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDDKIMPFKFKEVLDLTGKGSLSTRQKMRRVIGYIFRNLIKSKSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNLEFSRLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPSIVVNNEKRLLQESVDALFDNSSRHKPSLSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDENGNEIRPIAASIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAVLLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQLGEGIIFSTVYEARAAYESQKVHLHAIVGISTKAFPNKKFACQGTLITTVGKIIFNDVLGNNVPYINDGEFDENACPEKFIVKQGEDVRQSILKHQIIPAFSKKVISKLIDLLYLLLEFKDLPKTLDNIKALGFKYSTFSSTTVSVFDIPKYTNKQNYFDSADQQVLKYKQFYNKGLLTDDERYKRVVKLWNNVKEKVSDEIQNLIKQEQYRDNSIVVMADSGARCNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFFEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTKSLIESLFDRIVNRYSITPIVDPETQKTIVEANSLITTQLAKQICATSIKEVLVRSVIYCERENGICQYCFGIDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVTPKDFEKAVISEVKGTVKSITTVQNAQEVVIKSNVDERIYTIPFSAQIRVHVGDQVSPGSKITEGSVDIKQLLRIAGIQRVRQYMIVEIQKVYRIQGIDIADKYVEIIIRQLTNLLQVTDAGNSNLFVGQLVHSHYLNELNKSLLLAGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKNQVDYLLGLKENVIIGGKIPAGTGFLTDEELTFLGSKTVAEEY" }, "paralog": false, "identity": 0.9352272727272727, "score": 1634.0, "evalue": 0.0, "description": "Internal stop codon at 624647, 626897. Nonsense mutation around 624647, 626897.", "psc": { "uniref90_id": "UniRef90_P47582", "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47582", "cog_id": "COG0086", "cog_category": "K", "kegg_orthology_id": "K03046", "go_ids": [ "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47582", "EC:2.7.7.6", "UniRef:UniRef50_P47582", "COG:COG0086", "COG:K", "KEGG:K03046", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47582", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47582" ], "product": "DNA-directed RNA polymerase subunit beta'" } }, "id": "CNOCLNGIAL_1112", "locus": "GCA000143945_01108" }, { "type": "cds", "sequence": "contig_1", "start": 626895, "stop": 626987, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGCGTAATAAAAAGAACAACAAGCTGTACAAGAACATTAAGGCAATTAAGCTTTCGATTGCTTCCAACGACACGATCCTAAACTGA", "aa": "MTKRNKKNNKLYKNIKAIKLSIASNDTILN", "aa_hexdigest": "72f98435a436ea482b017ee4a2a46954", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3476.1007, "isoelectric_point": 10.468972587585448 }, "id": "CNOCLNGIAL_1113", "locus": "GCA000143945_01109" }, { "type": "cds", "sequence": "contig_1", "start": 626991, "stop": 627287, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04560.25" ], "nt": "TTGGAAGCTTACGGCGCTGCTTACAACCTTCAGGAATTACTTACCATTAAGTCCGATGATGTGCAGGGCCGGAATAAGGCCTATGCCGCGATCGTTAAGGGCGCTGCCTTCCCCGAACCAGGTATCCCCGAATCGTTTAAACTGTTAACGAAGGAACTACAAGGGTTAGCGTTGAGTGTGTCCTTTATTTACGATGACAACACCCAACAAGATTCCAACAACGTTTCAATTCTCCAAGCTGATGGAGAACAGGACGATCTCTTTAATGACTTTGAATTTGACACGGAGGGTTATTAA", "aa": "MEAYGAAYNLQELLTIKSDDVQGRNKAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQADGEQDDLFNDFEFDTEGY", "aa_hexdigest": "f83241a1831aaa94eba3c9c027ad662e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04560.25", "name": "RNA_pol_Rpb2_7", "length": 73, "aa_cov": 0.6428571428571429, "hmm_cov": 1.0, "evalue": 3.4276824203932016e-19, "score": 64.57222747802734, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 10832.72439999999, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_1114", "locus": "GCA000143945_01110" }, { "type": "cds", "sequence": "contig_1", "start": 627291, "stop": 628439, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "GTGATTTCCGAGCGCTTAGTGAAGGATGATGTTCTAACCTCCTTAACGATTAACGAATACGTAGCCCAGTGTTTATCGACCAAAAACGGTGATGAACAAATTACCCGTGATATCCCCAACGTTAGTGATGCTAACAAGCGTTACTTAGATGAAAACGGCATTATCATGGTCGGTGCTGAAGTTAAGGAAGGTGATGTCCTTGTCGGTAAGGTATCCCCTAAGGGACAGGTTGAAGTATCACCTGAAGAAAAGCTGTTTAAGGCCATTTTCCCTGAAAGTGTGCAAAACGTCCGTGACTCTTCCTTAAAGCTCCCTCATGGTGGTGATGGGATTGTGTCCTGTGTCAAGCGTTTTAGCATTGCCAACGGTAATGAACTTAATGATGGTGTCATCGAAATGATTAAGGTCTATGTGGTGCAAAAACGCAAGATCCAAATCGGAGATAAGTTGGCAGGGCGCCATGGTAACAAAGGGGTTATCTCTAAGGTTGTCCCAGTAGCGGACATGCCCCACTTAGAAGATGGTACTCCAGTCGACATCCTCTTAAACCCACTGGGGGTACCGAGTCGGATGAATATTGGCCAAATCTTTGAAATGCACTTGGGTTATGCAGCACATAACCTTGCCAAGCGAATGTTAATTAGTGCTTGTTTTGATGACAAGAAGGCTCAAGCACTTTCAACCGAAATTAACCAGCCACAGTACAAACTTGACCGCTTAATTACTGGTTTAAAAGCCCAAATTACGAACCGCGGTCTTAAAGACGAGCAGGCAGCCTTGGCCCAACTCAATAACGGTGACATTGCTTTAGTGCTCAAGGAAATCGGCATGAGCTTTGATGACCTCCACTTTAAAGTAGCGACCCCGATCTTCCAAGGGGTGAACTTCCAAGACTTGCAAGACATCATGGATGAAGCAGGATTGAAACCAGCGGAAACGCACGGGAAGTTCAAGCTGATTGATGGTCGCACTGGTTTACCATTTGAAAAACCAATTTCCTTGGGAATCATGTACATGATGAAGTTAAACCACATGGTTGATGACAAGATCCATGCTCGCGCTGTCGGTCCTTACTCCAAGATTACCCAACAACCATTGGGTGGTAAATCACAAAATGGGGGCCAACGCTTTGGGGAAATGGAAGTGTGA", "aa": "MISERLVKDDVLTSLTINEYVAQCLSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKLPHGGDGIVSCVKRFSIANGNELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPVADMPHLEDGTPVDILLNPLGVPSRMNIGQIFEMHLGYAAHNLAKRMLISACFDDKKAQALSTEINQPQYKLDRLITGLKAQITNRGLKDEQAALAQLNNGDIALVLKEIGMSFDDLHFKVATPIFQGVNFQDLQDIMDEAGLKPAETHGKFKLIDGRTGLPFEKPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEV", "aa_hexdigest": "0313eaaf14e12719edb25ed8f4070b9e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 377, "aa_cov": 0.9712041884816754, "hmm_cov": 0.7559681697612732, "evalue": 9.582100001645811e-101, "score": 332.989501953125, "start": 1, "stop": 371 } ], "seq_stats": { "molecular_weight": 41965.923000000046, "isoelectric_point": 6.332909584045409 }, "id": "CNOCLNGIAL_1115", "locus": "GCA000143945_01111" }, { "type": "cds", "sequence": "contig_1", "start": 628467, "stop": 631166, "strand": "-", "frame": 1, "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "COG:COG0085", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "SO:0001217", "UniRef:UniRef50_P47715", "UniRef:UniRef90_P78013" ], "nt": "ATGTCACAAAAACCTAGTTTCTTTCAGAAAAAATACTCACCCACTGCTACCCGCCGTTACTATGGCAAAATTGCCACTGATTTTGTGCAACCTAATCTGGCTGATATTCAGATTAGGAGCTATCAAACTTTTCTAGATCACGATCTGGAAAACCTGATTGCTGCTTATTTTCCAATTAAGTCCCCTAATGATCGTTACACGATCAACTTTAAGGGTTTGCGTCGTACCGCTCCTGAACGTAATGAAGCCCAATCACGCTCTGAGTCCAAGACCTATGAAATTGGTATTTACGCTGACTTAGAATTAATTGATTCCGCTACCGGAACCATTAAAAAGCCGCGCAAATCCAAGAAGAACAGTGCCACCAGTAGTGTCGATGGCGTTTTCTTAACTAACTTACCATTGATTACCCGCGATGGGGTCTTTATTGTCAACGGGATTGAGAAGTTTGTGATTGCCCAAATTACCCGTTCACCGGGGATCTACATGCTCACTAAGTCCCAGTTAAAATTGTCTAGTTCGCGTAAACGGGTACAAGAAGGTTATGTATGTGAAGTATTACCAGCCAACGGTTCGGTAATGCTGATCTACATCTCCAATAAAAAGAAGATTGAAGATGCCTTTGTGCAAATCTTGCTCCGGGATGCTGTACGTGAAGGGGCAAAGATCTTCCCGATTACCACCTTGCTCAAAGCCTTTGGGATGAGTGGCAAGGAAATCCTCAAAGTTTTCAAAAACAACGAGTTTATTACCCGTTCCTTAGAAGCGGAAGTCTACAACGCTAAGGATTTCTTAAACAACGTTGACCCTGAAATTAAAAACTTATTAAGGGAATTCCGCGATGGCAAAACTGATCTCCGCCGTAAGGGGATTGCCTCTGACCAAAAGATCCGCAGCTTAGTGAGTGACTATGTCCTTTTAGAAAAGGAACACAAAGCGCTCAGTGAAGCTAAACCAAATGACCCTAAAGTAGGTCAGTTAGAAGCGGACATGGATGAGTTGATGGATAAAATCATCACCGAACGGGCTGCTAAACACATTGTTCATGAATTGTCCATTTCACTACGTGGTTTAGAAAACACCGATGAATGTCCGGAAAACAGTTACCACGCTTTACTGTGTTCACGTTTCTTCCGTCAAAGAAGGTATAACCTCTCCGCTGCTGGTCGTTATAAGGTATCACGTAAGTTAAGAATTACCGAGCGGATTTACCAAAAAACACTTGCTTGTGACCTTCATTTAAAAAACGGGGAACTCTTATTAAAGAAGGGCACCTTATTAGTTAAAGAAGAAATCGACAAGATTAAGCAAGCTGCCCAAAACAACCAAATTGACTTTGTCCAAAAGATTAAGTTAACCACTGATGGTAGTGCTGTTAACCTTTCCCCTGAATCGTTGTTGTATGAATCACTTGATGTTTATGTGAACAACGACAACTTCGATGTATCCGTGCCAGTGGTGGGGATCCACAACGATAACGATCTCAATAAGGCAATTACTTTAAGTGACTTTATTGCCTCGATTAGTTATGTGATTAACATCCCTTCGGCCATCGGTAAGTACGATGACATTGATCACTTAGGTAACAAACGCGTCAAGTTAATTAACGAGTTAATTAGTTCCCGTTTGGAAAGTGGAATCACCCGCATGGAGCGCTTCTTAAAGGAAAAGCTCACGATTGCTGATGGAGTGAACCGTGGTCAACAAATTAACGAAGAGGGTCAAGTAATTGAACAAGCGGAAAAGAAGGAATTGACCATTAAATCCTTAATTAATTCCAAACCTATTCAAATTGTCATTCGTGACTTCTTCAACACTCACCAGTTGACCCAGTTCTTAGATCACCAAAATCCACTTTCAGAATTAAGTAATAAGCGCCGGATTTCCGCCATGGGTCCAGGGGGTATCTCCCGCGAAGACCCGAACTTGGATATCCGGGATGTGCACTATTCCCAATATGGTCGGATTTGTCCAATTGAAACACCGGAAGGGATGAACATTGGTTTAATCATGTCGTTGGCTTCCTTTGCCAAGATTGACGAAAACGGATTCCTAATGGCGCCTTACCGCAAGATTAAAAATGGTGTCATTACCGATGAAGTTGAATACCTCACGGCATTAAGGGAAGACGAGCACATTATTGCCGAAATCTCTTCGTTGGTCAACATTGACGAAAACAACAAGATCTTAGATAAGGAAATTATTGGTCGTTACCGCTCGATGCAAGGGTTGTATGATCCCTCGAAGATTGACTACATCGACGTGGCCCCTCATCAGGTAGTGTCCATCGGTTCGTCCTTAATTCCCTTCTTGGAAAACGATGACTCAGCGCGGGCATTGATGGGTACCAACATGCAACGCCAAGCCTATCCTTTAATTAAGCCTTACGCTCCCGTTGTGGGTACAGGTCAAGAGTACAAGATTGCTCGCGACTCCGGTTTGACGATGTTAGCACCTTGCTCAGGTACGGTAAAGTATGTAGACAACAGTAAGATCACGATTGAAAGTGACAGTGGTGAACAGCACACTTTGGATCTAATTAAGTTTGAACGTTCCAACCAAAATACCTGCTACAACCACGTTCCCTTAGTAGAAAAGGGGCAACGGGTAACTAAGGACGAAGTGATTGCTGATGGTCCTGCAGTTAACAAGAGTGAGCTGTCTTTAGGGCAAAATGTCCTTGTCGCCTTTACGACCTGA", "aa": "MSQKPSFFQKKYSPTATRRYYGKIATDFVQPNLADIQIRSYQTFLDHDLENLIAAYFPIKSPNDRYTINFKGLRRTAPERNEAQSRSESKTYEIGIYADLELIDSATGTIKKPRKSKKNSATSSVDGVFLTNLPLITRDGVFIVNGIEKFVIAQITRSPGIYMLTKSQLKLSSSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGMSGKEILKVFKNNEFITRSLEAEVYNAKDFLNNVDPEIKNLLREFRDGKTDLRRKGIASDQKIRSLVSDYVLLEKEHKALSEAKPNDPKVGQLEADMDELMDKIITERAAKHIVHELSISLRGLENTDECPENSYHALLCSRFFRQRRYNLSAAGRYKVSRKLRITERIYQKTLACDLHLKNGELLLKKGTLLVKEEIDKIKQAAQNNQIDFVQKIKLTTDGSAVNLSPESLLYESLDVYVNNDNFDVSVPVVGIHNDNDLNKAITLSDFIASISYVINIPSAIGKYDDIDHLGNKRVKLINELISSRLESGITRMERFLKEKLTIADGVNRGQQINEEGQVIEQAEKKELTIKSLINSKPIQIVIRDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKNGVITDEVEYLTALREDEHIIAEISSLVNIDENNKILDKEIIGRYRSMQGLYDPSKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPVVGTGQEYKIARDSGLTMLAPCSGTVKYVDNSKITIESDSGEQHTLDLIKFERSNQNTCYNHVPLVEKGQRVTKDEVIADGPAVNKSELSLGQNVLVAFTT", "aa_hexdigest": "ba37769ed89d798322ab4f5e11afd33b", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoB" ], "truncated": "5-prime", "pseudogene": { "start": 628164, "stop": 631166, "observations": { "mutation": [ 628469 ], "stop": [ 628469 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78013", "query_cov": 1.0, "subject_cov": 0.6458333333333334, "identity": 1.0, "score": 1738.0, "evalue": 0.0, "gene_start": 1, "gene_end": 899, "reference_start": 1, "reference_end": 899, "reference_sequence": "MSQKPSFFQKKYSPTATRRYYGKIATDFVQPNLADIQIRSYQTFLDHDLENLIAAYFPIKSPNDRYTINFKGLRRTAPERNEAQSRSESKTYEIGIYADLELIDSATGTIKKPRKSKKNSATSSVDGVFLTNLPLITRDGVFIVNGIEKFVIAQITRSPGIYMLTKSQLKLSSSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGMSGKEILKVFKNNEFITRSLEAEVYNAKDFLNNVDPEIKNLLREFRDGKTDLRRKGIASDQKIRSLVSDYVLLEKEHKALSEAKPNDPKVGQLEADMDELMDKIITERAAKHIVHELSISLRGLENTDECPENSYHALLCSRFFRQRRYNLSAAGRYKVSRKLRITERIYQKTLACDLHLKNGELLLKKGTLLVKEEIDKIKQAAQNNQIDFVQKIKLTTDGSAVNLSPESLLYESLDVYVNNDNFDVSVPVVGIHNDNDLNKAITLSDFIASISYVINIPSAIGKYDDIDHLGNKRVKLINELISSRLESGITRMERFLKEKLTIADGVNRGQQINEEGQVIEQAEKKELTIKSLINSKPIQIVIRDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKNGVITDEVEYLTALREDEHIIAEISSLVNIDENNKILDKEIIGRYRSMQGLYDPSKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPVVGTGQEYKIARDSGLTMLAPCSGTVKYVDNSKITIESDSGEQHTLDLIKFERSNQNTCYNHVPLVEKGQRVTKDEVIADGPAVNKSELSLGQNVLVAFTTWNGYNYEDAIVISERLVKDDVLTSLTINEYVAQCLSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKLPHGGDGIVSCVKRFSIANGNELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPVADMPHLEDGTPVXDILLNPLGVPSRMNIGQIFEMHLGYAAHNLAKRMLISACFDDKKAQALSTEINQPQYKLDRLITGLKAQITNRGLKDEQAALAQLNNGDIALVLKEIGMSFDDLHFKVATPIFQGVNFQDLQDIMDEAGLKPAETHGKFKLIDGRTGLPFEKPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEVWALEAYGAAYNLQELLTIKSDDVQGRNKAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQADGEQDDLFNDFEFDTEGY" }, "paralog": false, "identity": 0.999, "score": 1950.0, "evalue": 0.0, "description": "Internal stop codon at 628469. Nonsense mutation around 628469.", "psc": { "uniref90_id": "UniRef90_P78013", "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47715", "cog_id": "COG0085", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78013", "EC:2.7.7.6", "UniRef:UniRef50_P47715", "COG:COG0085", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_P47715", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47715" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "CNOCLNGIAL_1116", "locus": "GCA000143945_01112" }, { "type": "cds", "sequence": "contig_1", "start": 631259, "stop": 631759, "strand": "-", "frame": 2, "gene": "ssuE", "product": "putative MG342-like protein", "db_xrefs": [ "COG:C", "COG:COG0431", "GO:0005524", "GO:0005829", "GO:0010181", "GO:0016491", "RefSeq:WP_010874873.1", "SO:0001217", "UniParc:UPI000013965D", "UniRef:UniRef100_P75270", "UniRef:UniRef50_P75270", "UniRef:UniRef90_P75270" ], "nt": "ATGAGTACCAAACCGTTAATCTTATTGTTAGCTAACTCCAAAAATTCAATTAACCGGAAGTTTGCTAAAGCTTTGGAACAACAACTCAATGCTGAATTAATTGAACTAGTTGACTACCAAGTGGATTTTTATTGTGAAGATTTGGAAAAGGATCATTTTCCCGAAAAAATTAAGAGTTTAGTGCGCAAACTACACGATCACAAAACGCTAATTTTTGTCACTCCAGAACACAACGGTTTTGTCCCTGCCTTTGCTAAAAACACAATCGACTGGATGACCCGCGATACCCAATACGGTAAAAACCAGTTTTTAAAGGAGCTTGATGGAATTATTTGCTGTGTCACTCCTGCAGCTAAGAGTGGTGGTAAGACAGTGCTGGAACTACTCACTAAATTCTTTAGTTTTAGTGGTCTCAACGTTAAGGGAGCCGTGTTAGTAAATGGCTATCATGATGGCTTTGATTTTCAGCCATTTATCACTGACGTACAAAAATTGATATAA", "aa": "MSTKPLILLLANSKNSINRKFAKALEQQLNAELIELVDYQVDFYCEDLEKDHFPEKIKSLVRKLHDHKTLIFVTPEHNGFVPAFAKNTIDWMTRDTQYGKNQFLKELDGIICCVTPAAKSGGKTVLELLTKFFSFSGLNVKGAVLVNGYHDGFDFQPFITDVQKLI", "aa_hexdigest": "e2a5d7c7594bd7c1481c1e3a67847f94", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013965D", "ncbi_nrp_id": "WP_010874873.1", "uniref100_id": "UniRef100_P75270", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013965D", "RefSeq:WP_010874873.1", "UniRef:UniRef100_P75270" ] }, "ips": { "uniref100_id": "UniRef100_P75270", "uniref90_id": "UniRef90_P75270" }, "psc": { "uniref90_id": "UniRef90_P75270", "gene": "SsuE", "product": "Uncharacterized protein MG342 homolog", "uniref50_id": "UniRef50_P75270", "cog_id": "COG0431", "cog_category": "C", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0010181", "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75270" ], "product": "Uncharacterized protein MG342 homolog" }, "genes": [ "ssuE" ], "id": "CNOCLNGIAL_1117", "locus": "GCA000143945_01113" }, { "type": "cds", "sequence": "contig_1", "start": 632189, "stop": 632407, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6P5", "UniRef:UniRef90_A0AB38W6P5" ], "nt": "GTGAAAGTTTTGGTAGTTGTGAAACTAATAAAAAAAGATGTTTATTGTTTTCAATTCTTATTTAAGAAAAATTTAGAGGAAAACTTAGAGGATGATCATAAAGAAGAGGTATTGGATAGAATAGAAGGTTTATTCAAAAGTCTAAGAAAGGGAGGAAGCTTTAAAGAAGACGATCAGTCTAAAGATTTAATTAAGCATTTAAAAAGCATTAAAAAATGA", "aa": "MKVLVVVKLIKKDVYCFQFLFKKNLEENLEDDHKEEVLDRIEGLFKSLRKGGSFKEDDQSKDLIKHLKSIKK", "aa_hexdigest": "f9b4401fcf027851af0d30774ad05cf4", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 631994, "stop": 632407, "observations": { "mutation": [ 632029, 632101, 632191 ], "stop": [ 632029, 632101, 632191 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W6P5", "query_cov": 1.0, "subject_cov": 0.5255474452554745, "identity": 1.0, "score": 140.0, "evalue": 2.36e-41, "gene_start": 1, "gene_end": 72, "reference_start": 1, "reference_end": 72, "reference_sequence": "MKVLVVVKLIKKDVYCFQFLFKKNLEENLEDDHKEEVLDRIEGLFKSLRKGGSFKEDDQSKDLIKHLKSIKKWSENEDKKHLKELKKYLKSSMDTKLPSEDHWNAKEAKERLASAKKDALLELDSEWYTTFDHDKRE" }, "paralog": false, "identity": 0.9708029197080292, "score": 260.0, "evalue": 3.12e-91, "description": "Internal stop codon at 632029, 632101, 632191. Nonsense mutation around 632029, 632101, 632191.", "psc": { "uniref90_id": "UniRef90_A0AB38W6P5", "uniref50_id": "UniRef50_A0AB38W6P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W6P5", "UniRef:UniRef50_A0AB38W6P5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6P5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8552.959799999993, "isoelectric_point": 9.015017127990724 }, "id": "CNOCLNGIAL_1118", "locus": "GCA000143945_01114" }, { "type": "cds", "sequence": "contig_1", "start": 632857, "stop": 633102, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTGTATTTTGAAAACTTGCAAGTGTTTGCCCAAGAAGCTAGCACTTTACAAGTTAATGCAATTGTGATTAAACAAGATGCCTATTTTATTGTTGAAGTAATGAAGGGTTACAACTATGACAATAATTGCATCTGTTTATTAGAGGCCAATAAAGATCAAAACGTTGGTTTAACCACCGTAAGCACTAATACTCTAATAGGTGACATCAAGCAATACGAGTCTTGTTTCTTTATTGAAAAGTAA", "aa": "MLYFENLQVFAQEASTLQVNAIVIKQDAYFIVEVMKGYNYDNNCICLLEANKDQNVGLTTVSTNTLIGDIKQYESCFFIEK", "aa_hexdigest": "daea10d0ea47644c039ef374941ec279", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9270.480299999996, "isoelectric_point": 4.31762638092041 }, "id": "CNOCLNGIAL_1119", "locus": "GCA000143945_01115" }, { "type": "cds", "sequence": "contig_1", "start": 633136, "stop": 633303, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAACAGTAACAGTTTTGACCTGAGCTTTACCAGTTTCAAAGAACGCTTTAATACTGAAAATAACTTAGACATTGATGTGCACTTCAACGTACCACACAAGTATTTACTCAAAAACTTGGAGAGTATTAAGCACCACTTACAAATAGATAACAATACCATTTGA", "aa": "MANSNSFDLSFTSFKERFNTENNLDIDVHFNVPHKYLLKNLESIKHHLQIDNNTI", "aa_hexdigest": "07d8dacefc174e194b21150a25eeb753", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6505.159099999999, "isoelectric_point": 5.997389030456542 }, "id": "CNOCLNGIAL_1120", "locus": "GCA000143945_01116" }, { "type": "cds", "sequence": "contig_1", "start": 633330, "stop": 633902, "strand": "-", "frame": 1, "gene": null, "product": "putative MG343-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75269", "UniRef:UniRef90_P75269" ], "nt": "ATGGCACAAACAACCATTAACGAGCAAACCTTGGTGAATGAGCAAAGACTTTTAGCTTACGATTGCTTTCAAAACACCAATAAAGTGGGATTACTTTCCGCTTTAGAATACTTAGATTTTGTCAATCACCTAGTTAAGTCGAATCAGATTAAATACTTATTAGTACCATCAGCGAGTCAAACAGTTTACCAACACGTTTTCTTTGATCACTTTCGCTTTTTAAGTTTTGACAATAACTTATTAAGTGGTTTTGGTTTAGCGATTAGGTTACATAAAGAAGCGGAAACAGTGTTTATGTTGGTCGAAAAAACCAAACAAACAGAAACAGAAATTAAAAAGCTGTTAGAAGCAGTTAAACAGTTTTCCGGGCTGAAATTAGTGGTTTTACTAGTTAATTCTCCGTTTGAAAATAAGCGAACAAAGCTTTCTTATGAAATCGTTAAAATCATTAAAAAGCACGGTTATCAGTACAAACGTTATTGCCGCAAAAACTTTGAAAAGAAGTTTTTAATACTTGGAACCACCAAAAGCGCAAGCAAAAAACAAAGAATAACCTTTTCTTTGTCGAGTTAA", "aa": "MAQTTINEQTLVNEQRLLAYDCFQNTNKVGLLSALEYLDFVNHLVKSNQIKYLLVPSASQTVYQHVFFDHFRFLSFDNNLLSGFGLAIRLHKEAETVFMLVEKTKQTETEIKKLLEAVKQFSGLKLVVLLVNSPFENKRTKLSYEIVKIIKKHGYQYKRYCRKNFEKKFLILGTTKSASKKQRITFSLSS", "aa_hexdigest": "93d83d9a732a4978917e0dddda6990dd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 633028, "stop": 633902, "observations": { "deletion": [ 633395 ], "stop": [ 633137 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75269", "query_cov": 0.8894736842105263, "subject_cov": 0.48563218390804597, "identity": 1.0, "score": 326.0, "evalue": 6.79e-110, "gene_start": 1, "gene_end": 169, "reference_start": 1, "reference_end": 169, "reference_sequence": "MAQTTINEQTLVNEQRLLAYDCFQNTNKVGLLSALEYLDFVNHLVKSNQIKYLLVPSASQTVYQHVFFDHFRFLSFDNNLLSGFGLAIRLHKEAETVFMLVEKTKQTETEIKKLLEAVKQFSGLKLVVLLVNSPFENKRTKLSYEIVKIIKKHGYQYKRYCRKNFEKKFFNTWNHQKRKQKTKNNLFFVELNSQFAFGTSMANSNSFDLSFTSFKERFNTENNLDIDVHFNVPHKYLLKNLESIKHHLQIDNNTIWKQAFINFAGQLLLYFENLQVFAQEASTLQVNAIVIKQDAYFIVEVMKGYNYDNNCICLLEANKDQNVGLTTASTNTLIGDIKQYEPCFFIEK" }, "paralog": false, "identity": 0.9965635738831615, "score": 570.0, "evalue": 1.69e-208, "description": "Internal stop codon at 633137. Frameshift due to deletion around 633395.", "psc": { "uniref90_id": "UniRef90_P75269", "product": "Uncharacterized protein MG343 homolog", "uniref50_id": "UniRef50_P75269", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75269", "UniRef:UniRef50_P75269" ] }, "pscc": { "uniref50_id": "UniRef50_P75269", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75269" ], "product": "Uncharacterized protein MG343 homolog" } }, "id": "CNOCLNGIAL_1121", "locus": "GCA000143945_01117" }, { "type": "cds", "sequence": "contig_1", "start": 633905, "stop": 634213, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGTTAGACAAGCATACTGCTATGTGCAAAAAACTCACCTTTCAGATCTTTAAGGAATTGTCGCGCTTAAACAGTGCTTATAAAACTATTACCATACCAGCCTTTTTACTGTTGGCTCAACATGATGATTTTATGCCTACAAAAGCTACTTTAAACTACTTTAACCGCTTTTTGATTAAGAAAGGTAACTTGCAATCAGGGGTTATCTTACACAGTCAACACCAAATGTTTAACAGCAAGCATGAAAGTTTCTGTAAAGCAATGCACGATATTTTGAAGCATAATAAACTAGGTAAAATTTACTAG", "aa": "MELDKHTAMCKKLTFQIFKELSRLNSAYKTITIPAFLLLAQHDDFMPTKATLNYFNRFLIKKGNLQSGVILHSQHQMFNSKHESFCKAMHDILKHNKLGKIY", "aa_hexdigest": "a1412fdb4e7d4ac05661b57805b6ea68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11941.95259999999, "isoelectric_point": 9.69825305938721 }, "id": "CNOCLNGIAL_1122", "locus": "GCA000143945_01118" }, { "type": "cds", "sequence": "contig_1", "start": 634217, "stop": 634423, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGGGCAGTGGCTAGTTATGCATTGCAGTTTTTAAAACCACAACGAGTGGAAAAACTAGTGCTCTTAGCGCCACTGTCTTATTCCAACTTGTTGAATTACTACAAGATTAAAAAGGCTTTTAAAAAGAAGGACGAAAAGCTGTCTTACTTTAAGCGGATGTTTCAACCAAAGTTTCCTGATTTACAAAACGATGGACAGTGA", "aa": "MGGAVASYALQFLKPQRVEKLVLLAPLSYSNLLNYYKIKKAFKKKDEKLSYFKRMFQPKFPDLQNDGQ", "aa_hexdigest": "5aab33eaa30b1b6da90de7cda195fd41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7946.292999999999, "isoelectric_point": 9.889272880554199 }, "id": "CNOCLNGIAL_1123", "locus": "GCA000143945_01119" }, { "type": "cds", "sequence": "contig_1", "start": 634544, "stop": 634723, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAGACAAAGTCAATATTAATACTGTTGATATTAGCCAACTAGAAGTATTTTTTCAACCAGCTAAAAAACCCGCTAAACAAACTATTGTGTTTGCGCACGGTTTTTCAGTGCACCATTCTTATTTTAAGAGCTTTTCTGAAACGCTGGTGGATTATGACTATTACGCACCCTTGTGA", "aa": "MRDKVNINTVDISQLEVFFQPAKKPAKQTIVFAHGFSVHHSYFKSFSETLVDYDYYAPL", "aa_hexdigest": "f3be089e3822adf74f66f4f7186c04ae", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6869.7207, "isoelectric_point": 6.813312721252441 }, "id": "CNOCLNGIAL_1124", "locus": "GCA000143945_01120" }, { "type": "cds", "sequence": "contig_1", "start": 634726, "stop": 635130, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAAAAAGCCGGTTAGTGTAAACCTGTTTGCAGGACCAGCCATGTTTAAAGTTGCTAATGTTAAAGGGTTAGATCGCTTACACGAAAGCTTTAGCGCCATTAAAGACCGGGCTTATGCAGCCATTGAACAAGCACGTCAAAATGGTGTGATTACTAAAAACAACCAGGTTGTATTGACATTGGGTGTGGATTCTACTAGTGCAATTGATCAACGTCTTGTAAAACATTTAGCAGTTTGGCTAAACGTAAACCAGGTAAATATAACGAATGACCCCAACGAAATTAAGGTTGAACACACCGATCAAGTCATGTGTGAACGATGTTGGAACTTCCAAACCCAAATTTACCCTAAAAAAGGACACCAATTGTGTGCAAGGTGTTTTACTTTTCTTAAAAGGTAA", "aa": "MPKKPVSVNLFAGPAMFKVANVKGLDRLHESFSAIKDRAYAAIEQARQNGVITKNNQVVLTLGVDSTSAIDQRLVKHLAVWLNVNQVNITNDPNEIKVEHTDQVMCERCWNFQTQIYPKKGHQLCARCFTFLKR", "aa_hexdigest": "4f70531f0f68287ee56a6c328711dd4e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15209.438499999995, "isoelectric_point": 9.474805641174317 }, "id": "CNOCLNGIAL_1125", "locus": "GCA000143945_01121" }, { "type": "cds", "sequence": "contig_1", "start": 635143, "stop": 635556, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "GTGGACAACCGCATAGGTGACAACATTATCAAGCAAATTGTCGAGCAATACCGCCGCATTCGCAACAGCTTATTGCGCTTTATATTGGGTAACCTCAACCACTTTAACTTTGGTGAGATGAAGGATTATCGTTTTGCTCTAGAGGACAAAATCGTAATCCATAAAACTAATGCATTGGTACAAGAATTGCACCAGTGGTTAAAGCAATACAACTTTTTAAATTGCTTAAAGGCCATTAATAAGTTTGTGTTGTGGTTATCGGGTTGGTATTTTGAAATTATTAAGGACACACTTTATTGTGATGCTAAAACTAATCCTAATCGGTTGGCTAAGCAGGCAGTATTGAACTATATCTTTACCCAGTTAATTGGTTTCTTAAATATCTTTATTCCCCATACTGCCGAAGATGCTTGA", "aa": "MDNRIGDNIIKQIVEQYRRIRNSLLRFILGNLNHFNFGEMKDYRFALEDKIVIHKTNALVQELHQWLKQYNFLNCLKAINKFVLWLSGWYFEIIKDTLYCDAKTNPNRLAKQAVLNYIFTQLIGFLNIFIPHTAEDA", "aa_hexdigest": "98e8e502d76f64856c5f398eb523986b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 89, "aa_cov": 0.6423357664233577, "hmm_cov": 0.9550561797752809, "evalue": 9.806747121845995e-18, "score": 59.92002868652344, "start": 49, "stop": 136 } ], "seq_stats": { "molecular_weight": 16338.79909999999, "isoelectric_point": 9.120100593566896 }, "id": "CNOCLNGIAL_1126", "locus": "GCA000143945_01122" }, { "type": "cds", "sequence": "contig_1", "start": 635560, "stop": 635862, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGAAGTATGGTTTGACTCTGGTTCAACTTACAACGTTTTAATTAGTAATAAACTGAACTTTCCCGCTGATCTTTATTTGGAGGGTAGTGACCAATACCGCGGTTGGTTTAACTCATCCGCCAGTTGCGGTATTATCCAAACTGACCAATTACCCTTCAAGAGTTTGATTTCCCATGGTTTTACCTTGGATGAACATGGTAATAAGATGTCCAAGTCACTCGGTAATGTGGTCGACCCTTTAAAGTTGTGTGATCAGTATGGCGCAGACATTCTGCGTTTATGGGTAACTAATGTTGATTGA", "aa": "MEVWFDSGSTYNVLISNKLNFPADLYLEGSDQYRGWFNSSASCGIIQTDQLPFKSLISHGFTLDEHGNKMSKSLGNVVDPLKLCDQYGADILRLWVTNVD", "aa_hexdigest": "8b3beaaa58ce2b6db2d7a4ebbed36ea8", "start_type": "TTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 106, "aa_cov": 0.98, "hmm_cov": 1.0, "evalue": 9.450598431671119e-35, "score": 115.5433578491211, "start": 1, "stop": 98 } ], "seq_stats": { "molecular_weight": 11237.48049999999, "isoelectric_point": 4.706518745422362 }, "id": "CNOCLNGIAL_1127", "locus": "GCA000143945_01123" }, { "type": "cds", "sequence": "contig_1", "start": 635913, "stop": 636041, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGATTCCCTTAATTTATGCAGATAACCAACCCTTACTGGATGTGACTACCATTAAATACACCATTCAACAGCTCAAAAAGTACGGGATTGACAGTTGGTTTGAAAAGGATATTAATTTTTTTTAA", "aa": "MPIPLIYADNQPLLDVTTIKYTIQQLKKYGIDSWFEKDINFF", "aa_hexdigest": "c8fdf7ed9fdc69088bf0137d60e34712", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5021.7786, "isoelectric_point": 4.777908515930175 }, "id": "CNOCLNGIAL_1128", "locus": "GCA000143945_01124" }, { "type": "cds", "sequence": "contig_1", "start": 636045, "stop": 636212, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGATTTACCGTGCCTCCAAACAGCTCTTCATTAAAACTAAATCAATTCAAAGTAAGCTAAAGCGTCAAATTAAAAGGGTTAAGTTTGTTAATAACAAGAATAAAGAGCGCCTACAAGAAATGCTGTTGCAACGTGCTGAATGGTGTATTTCCAGACAACGAGTATGA", "aa": "MIYRASKQLFIKTKSIQSKLKRQIKRVKFVNNKNKERLQEMLLQRAEWCISRQRV", "aa_hexdigest": "252389a40024f64179a19fcfbe583db8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 54, "aa_cov": 0.9818181818181818, "hmm_cov": 1.0, "evalue": 4.279895449182233e-12, "score": 40.91628646850586, "start": 2, "stop": 55 } ], "seq_stats": { "molecular_weight": 6793.0944, "isoelectric_point": 11.394609642028808 }, "id": "CNOCLNGIAL_1129", "locus": "GCA000143945_01125" }, { "type": "cds", "sequence": "contig_1", "start": 636228, "stop": 636515, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACCCGGTGTTGTTGGGTAATCACGTTTTATGTAATGAGGGTACAGGACTCGTGCATACTTCACCAGCTTATGGTTTGGATGACTTTTATTTGTGCAAGCAAAACAAGTTAAATGAAGCGCTAGTTTCACTTGATGAAAAAGGAGTTTTTAATGATACGTTGAACGATCCAGTTTTAACGGGTTTGTTTTATCTAAAGGCTAATGATGTGATTATTGAACGCTTAAAGCAACACCATAACTTTGTCTTTAGTGAAAGCTTTTTGCACCGCGAACCCCACGATTGA", "aa": "MNPVLLGNHVLCNEGTGLVHTSPAYGLDDFYLCKQNKLNEALVSLDEKGVFNDTLNDPVLTGLFYLKANDVIIERLKQHHNFVFSESFLHREPHD", "aa_hexdigest": "fedd404a89f9918e5212c7f1fb1d71e8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10781.060099999992, "isoelectric_point": 5.345559501647949 }, "id": "CNOCLNGIAL_1130", "locus": "GCA000143945_01126" }, { "type": "cds", "sequence": "contig_1", "start": 636867, "stop": 637160, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGTTTATTCCTGGTTGGGATTGCCATGGTTTGCCCATAGAGCATGCGGTGAGCAAAAAAGATCCGCAACACTATGCGAGCTTATCATTGAGTGAAAAGCGCGATTTATGCAAGCAATTTGCCCTGTCACAAATTGCCATTCAAAAAGCCCAGTTTCAACGCTTAGGTCTGTTGAATGACTTTAGTAAGTATTACAAAACAATTGATGAAAGCTTTCAACAAAATGAGCTAGACCTGTTTTTACAAGCAGTGAAAAAGGATTTAATCTTCCAAGCGTTAAAACCAACTTACTGA", "aa": "MFIPGWDCHGLPIEHAVSKKDPQHYASLSLSEKRDLCKQFALSQIAIQKAQFQRLGLLNDFSKYYKTIDESFQQNELDLFLQAVKKDLIFQALKPTY", "aa_hexdigest": "6ba38bf4d506117874a4f2508a75eaf1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 98, "aa_cov": 0.9690721649484536, "hmm_cov": 1.0, "evalue": 5.025416380585223e-21, "score": 70.33189392089844, "start": 2, "stop": 95 } ], "seq_stats": { "molecular_weight": 11278.85899999999, "isoelectric_point": 7.773664283752441 }, "id": "CNOCLNGIAL_1131", "locus": "GCA000143945_01127" }, { "type": "cds", "sequence": "contig_1", "start": 637332, "stop": 637424, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTAAAAAAGACACTGTTAATGCCCCAAACTGCATTTGAAATGCAGGGCAAATTAACCACTAAGGAACAGCAGTTTCAAGCTTTTTGA", "aa": "MNLKKTLLMPQTAFEMQGKLTTKEQQFQAF", "aa_hexdigest": "f6888f38c3847dcbb77891cbd9482cf2", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3532.1588, "isoelectric_point": 9.524639701843263 }, "id": "CNOCLNGIAL_1132", "locus": "GCA000143945_01128" }, { "type": "cds", "sequence": "contig_1", "start": 637467, "stop": 637865, "strand": "+", "frame": 3, "gene": "trmL", "product": "tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL", "db_xrefs": [ "COG:COG0219", "COG:J", "EC:2.1.1.207", "GO:0002130", "GO:0003723", "GO:0005737", "GO:0006400", "GO:0008175", "GO:0141098", "GO:0141102", "KEGG:K03216", "SO:0001217", "UniRef:UniRef50_P47588", "UniRef:UniRef90_P75257" ], "nt": "ATGTATAAATCAGCGATTAATATCGTCTTATTTTGCCCAGAAATTCCTAATAACACTGGGAACATTGTTCGGAGCTGTACCGCTTTTAAAGCTAACTTGCATCTGATTAAGCCCTATGGCTTTTTTCTCAATGATAAGCGGATGGTGCGTGCTGGGTTAAATTGTTGGGATAAGGTGCAAATGTTTGAACACAAATCCTGGGAACACTTCATTGAAACAACACCACCAAATAAAGCAATTTGGTTGTTGACAAAGAGTGGTACTACTACCCCAGACCAAATTAACATGCAAACAGATAACAGGGAACAACTGTACTTTGTTTTTGGTCAAGAAACCAAGGGATTACCGCAAACACTCATGGAACAATACTCGCAGAACCAGGTACGGATTCCAATTTGA", "aa": "MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQMFEHKSWEHFIETTPPNKAIWLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLMEQYSQNQVRIPI", "aa_hexdigest": "f6f3fa676df99b31653d2ad76cc1e762", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmL" ], "truncated": "3-prime", "pseudogene": { "start": 637467, "stop": 637967, "observations": { "mutation": [ 637863 ], "stop": [ 637863 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75257", "query_cov": 1.0, "subject_cov": 0.7951807228915663, "identity": 1.0, "score": 283.0, "evalue": 1.49e-96, "gene_start": 1, "gene_end": 132, "reference_start": 1, "reference_end": 132, "reference_sequence": "MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQMFEHKSWEHFIETTPPNKAIWLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLMEQYSQNQVRIPIWNPVRSINLSNTVACVLYEYAKQNHYFNLDKQCA" }, "paralog": false, "identity": 0.9939759036144579, "score": 347.0, "evalue": 3.31e-124, "description": "Internal stop codon at 637863. Nonsense mutation around 637863.", "psc": { "uniref90_id": "UniRef90_P75257", "gene": "trmL", "product": "tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_P47588", "cog_id": "COG0219", "cog_category": "J", "kegg_orthology_id": "K03216", "go_ids": [ "GO:0002130", "GO:0003723", "GO:0005737", "GO:0006400", "GO:0008175", "GO:0141098", "GO:0141102" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75257", "EC:2.1.1.207", "UniRef:UniRef50_P47588", "COG:COG0219", "COG:J", "KEGG:K03216", "GO:0002130", "GO:0003723", "GO:0005737", "GO:0006400", "GO:0008175", "GO:0141098", "GO:0141102" ] }, "pscc": { "uniref50_id": "UniRef50_P47588", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47588" ], "product": "Putative tRNA (cytidine(34)-2'-O)-methyltransferase" } }, "id": "CNOCLNGIAL_1133", "locus": "GCA000143945_01129" }, { "type": "cds", "sequence": "contig_1", "start": 637958, "stop": 638299, "strand": "+", "frame": 2, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_P47589", "UniRef:UniRef90_P75256" ], "nt": "GTGCGCTTAAGAAAAGTTAAAGATGCTCTTGTAAGGGTACAAGACAGCCCTTACTTTAGTAACCCAGAACAACTGTCAACTATTGAAAGTGAATCGCTCTTCGTGGAAATAGGTTGTGGTAAAAGTTGGTTTTTAAGCCAGCAAGCACAGCAAAACCCAAAGTGTTTTTTTGTGGGAATCGAGCGTGAACCCACGATTGTTTTAAAGGCAATTAATAAGGTAAACCGCTTAGAGGTTAAACCACGTAATTTATTAATAACGTGTTTGGATGCCAACCAGCTAACAGAGTACTTAAAACCACAATCGATTAGCAAAATCTTCATTAACTTCCCCGATCCTTGA", "aa": "MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDP", "aa_hexdigest": "51c22b221694814d10f5d606558cc1e1", "start_type": "GTG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 637958, "stop": 638590, "observations": { "mutation": [ 638297 ], "stop": [ 638297 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75256", "query_cov": 1.0, "subject_cov": 0.5380952380952381, "identity": 1.0, "score": 229.0, "evalue": 4.51e-75, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDPWPKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN" }, "paralog": false, "identity": 0.9857142857142858, "score": 412.0, "evalue": 2.11e-149, "description": "Internal stop codon at 638297. Nonsense mutation around 638297.", "psc": { "uniref90_id": "UniRef90_P75256", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_P47589", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75256", "EC:2.1.1.33", "UniRef:UniRef50_P47589", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_P47589", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47589" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "CNOCLNGIAL_1134", "locus": "GCA000143945_01130" }, { "type": "cds", "sequence": "contig_1", "start": 638318, "stop": 638590, "strand": "+", "frame": 2, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_P47589", "UniRef:UniRef90_P75256" ], "nt": "TTGAGGCGACTAACTGCTCCACATTTCCTCAAGCAGTTTCACCAACTTTTAAAAAAGCAAGGTTTAATAGAATTCAAAACGGACAACGATCAGTTGTTTGAATTTACCTTAGAAGTCTTGCAGAAAACAGTAGCTCACTTTAAGATAATTGAGCAAACGACAGATCTGCACAATTCACCTTTGGCTAGTACCAATATTATGACCGAATACGAACAGCGCTTTGTTAGTTTAGGGGTTAAGATTAAAAAGCTAACGCTTGAAAAACTAAATTAG", "aa": "MRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPLASTNIMTEYEQRFVSLGVKIKKLTLEKLN", "aa_hexdigest": "04d7ec92c2dda8675b7d836dbeb7c203", "start_type": "TTG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "5-prime", "pseudogene": { "start": 638018, "stop": 638590, "observations": { "mutation": [ 638297 ], "stop": [ 638297 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75256", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 0.978, "score": 176.0, "evalue": 2.37e-54, "gene_start": 1, "gene_end": 90, "reference_start": 121, "reference_end": 210, "reference_sequence": "MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDPWPKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN" }, "paralog": false, "identity": 0.9894736842105263, "score": 375.0, "evalue": 2.53e-135, "description": "Internal stop codon at 638297. Nonsense mutation around 638297.", "psc": { "uniref90_id": "UniRef90_P75256", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_P47589", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75256", "EC:2.1.1.33", "UniRef:UniRef50_P47589", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_P47589", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47589" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "CNOCLNGIAL_1135", "locus": "GCA000143945_01131" }, { "type": "tRNA", "sequence": "contig_1", "start": 638620, "stop": 638695, "strand": "+", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 71.9, "nt": "GCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 638626, 638628 ], "id": "CNOCLNGIAL_15", "locus": "GCA000143945_01132" }, { "type": "cds", "sequence": "contig_1", "start": 638744, "stop": 639109, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTTTTATTCAAACAGTTTACAGCGGTAGTGTCAATAATCTTATTAACCAGTCCAATGTTCATGCCCAAACGGTTGGCACTGATGTAACTAAAGCAATTGTGATTGCTTTTCGCAAAAACAATTTAGATCAAATTAGTGCGCATTTCTATAATCCTGATAACAGTAACGGCAGTAATGCACCAGGTTCAAACCAACCAAATCAGGACAGTGGAAATAACGGTTCAACAACTCCAGCAGCTTCTGCTGCAGCCGCCGCTAAAAAATCCTATTCAGCTGGATCATTTGGCGTTAAACGCCATGCCGTGCAGGTAAGCATTAACTTTCTCAATTTCTTGGATAGTGTTTACACACCCTTAAACTAG", "aa": "MTFIQTVYSGSVNNLINQSNVHAQTVGTDVTKAIVIAFRKNNLDQISAHFYNPDNSNGSNAPGSNQPNQDSGNNGSTTPAASAAAAAKKSYSAGSFGVKRHAVQVSINFLNFLDSVYTPLN", "aa_hexdigest": "3362dfeb7399439e13907057fb6f45be", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12749.794599999983, "isoelectric_point": 9.1006311416626 }, "id": "CNOCLNGIAL_1136", "locus": "GCA000143945_01133" }, { "type": "cds", "sequence": "contig_1", "start": 639341, "stop": 639661, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAGTGGTTTAGTTCAATTTCTAAAAAGAAAGTATCTTTTTCAACCTTACTCTTGTTAGGTTCAGGAATTGTTTTGAGTTCCTGTAGCAATATTGATAAACCAAATGTTTTTCGCACTCTTTCACAGCAAAGCGTAGAAAACAAAGTTGATTACAGTAAGCTACCTAAAGAAAATAAAACGGTTAGAAACTTAGTTTTTGGAACAGCGGAATACAATGATGGTAACTACGTTCTTGTAGTAACCACTGAAACTGACAGTTCTCAAATTAACTTTCTAAATGGTTCAAACAATCAAGCTGTTAGTACCGAAAATTGA", "aa": "MKKWFSSISKKKVSFSTLLLLGSGIVLSSCSNIDKPNVFRTLSQQSVENKVDYSKLPKENKTVRNLVFGTAEYNDGNYVLVVTTETDSSQINFLNGSNNQAVSTEN", "aa_hexdigest": "f436f68e8794e7874d1fb98a8b981eb7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11744.072799999993, "isoelectric_point": 9.09850368499756 }, "id": "CNOCLNGIAL_1137", "locus": "GCA000143945_01134" }, { "type": "tRNA", "sequence": "contig_1", "start": 639676, "stop": 639761, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(gag)", "amino_acid": "Leu", "anti_codon": "gag", "score": 65.6, "nt": "GTCGGAGTGGTGGAATGGTAGACACGCAAGCTTGAGGGGCTTGTGGTCGCAAGACTGTGCCAGTTCAAGTCTGGTCTCCGACACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 639755, 639757 ], "id": "CNOCLNGIAL_18", "locus": "GCA000143945_01135" }, { "type": "cds", "sequence": "contig_1", "start": 640250, "stop": 640588, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAAAGTTAAATACGTTACCCAAGAACAATTTCAGGAATATAAAGATTCTAACAATCAGCGACTCATAAAGATTGAAACCACTTTGGCGGCCCAAGGTGAGCAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGCGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGCTTGGACAATATGGAAGTAAAAATGGACAAGATGGAAGGAAAAATAGACAAAATAGAAAATAGAATGGACAAGATGGAAGTAAAAATGGACAAAATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAA", "aa": "MKVKYVTQEQFQEYKDSNNQRLIKIETTLAAQGEQIRIQGEQIKELQIEQKAQGETLKLILQTLQKMSDRLDNMEVKMDKMEGKIDKIENRMDKMEVKMDKMEKRIDKLESK", "aa_hexdigest": "c1066b8d5a31c1c84f898ab18541d637", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.6607142857142857, "hmm_cov": 1.0, "evalue": 7.575559163403e-27, "score": 89.36125183105469, "start": 2, "stop": 75 } ], "seq_stats": { "molecular_weight": 13365.423299999991, "isoelectric_point": 7.832409095764161 }, "id": "CNOCLNGIAL_1138", "locus": "GCA000143945_01136" }, { "type": "cds", "sequence": "contig_1", "start": 640625, "stop": 640735, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAAAAATTTCCGAAAAGGAATACAAAGCCTTAATCCGTAAAATTGGAAAAGAACACTTTGATGGTGAAAAAGAAGAGTACGGTGATGGCACAGTTGGAGTTTGA", "aa": "MKEKISEKEYKALIRKIGKEHFDGEKEEYGDGTVGV", "aa_hexdigest": "7ebb4537de1704cf08275a6cca7d1498", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4142.643400000003, "isoelectric_point": 5.72024211883545 }, "id": "CNOCLNGIAL_1139", "locus": "GCA000143945_01137" }, { "type": "cds", "sequence": "contig_1", "start": 641328, "stop": 641444, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGCCGAACTATTACCGTGTCATCAAGAAAGCAACCTCATTTTCAGGGTTAGATATGATCTCTCATGCCTATGGTCAGATTGCTGGCGTTGAAGTTGTGGGTTTTTATTTATGA", "aa": "MQPNYYRVIKKATSFSGLDMISHAYGQIAGVEVVGFYL", "aa_hexdigest": "b5707dbd8c72970a7499dee8c5e630f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4254.8816, "isoelectric_point": 8.119486808776855 }, "id": "CNOCLNGIAL_1140", "locus": "GCA000143945_01138" }, { "type": "cds", "sequence": "contig_1", "start": 641712, "stop": 642314, "strand": "+", "frame": 3, "gene": "dnaB2", "product": "putative MG349-like protein", "db_xrefs": [ "COG:COG3611", "COG:L", "KEGG:K03346", "SO:0001217", "UniRef:UniRef50_P75253", "UniRef:UniRef90_P75253" ], "nt": "ATGGGAAAACTGGAATATGACCGCAGCTGCCTTTCCTTTGAACAAATCGATAACATTCAGTTTGACAACGCACTGGAAATTACCGCTAACTTTGAAGCTAACTTTACCAGTAAGCAAGATTGTCTTAGCTTTAGTTTTGACTTTGAAGCTTTTCATAAACAATTAGTTAAACAAAATCTCTTTGTTAGTTTTAACCAGAAAACTAAGGCTGTTATTAATGGTTACTTTGAAAAATACCAAATAACACTAGAAGGCATATTGGAATGTGTTATCCCTAGTGTAGTTAATGATGAAGTTGACTTGGAACTGCTACAAAAATTACTAGAGCAGCTAGTTAAAAACAACACAGCTCCAATTGTTGATACTGTAATAAATGACCGCAACTTCTTTTATGATAATCAGAATTTAGCCACAGAAACAAAAGATGCAATTTCCCGTTGTCATTTGGAATACAACGCGGAAAAATACCTATTTCTCTTGTACGGTAAAGTAGAAAACGAGCAGTTAGATCTAATTCGCCGCTTAAGACAAGAGTTTGGAATAGCTGATAAGGTAATTAATCTAATAGTAGACTTCTCCTTTTGGAAAAACAACAGCATGTGA", "aa": "MGKLEYDRSCLSFEQIDNIQFDNALEITANFEANFTSKQDCLSFSFDFEAFHKQLVKQNLFVSFNQKTKAVINGYFEKYQITLEGILECVIPSVVNDEVDLELLQKLLEQLVKNNTAPIVDTVINDRNFFYDNQNLATETKDAISRCHLEYNAEKYLFLLYGKVENEQLDLIRRLRQEFGIADKVINLIVDFSFWKNNSM", "aa_hexdigest": "34fcba0d2bdc684b6bb5728a9e71afc9", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaB2" ], "truncated": "both", "pseudogene": { "start": 641412, "stop": 642569, "observations": { "mutation": [ 641442, 641556, 641640, 641661, 642312 ], "stop": [ 641442, 641556, 641640, 641661, 642312 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75253", "query_cov": 1.0, "subject_cov": 0.48426150121065376, "identity": 0.995, "score": 396.0, "evalue": 2e-136, "gene_start": 1, "gene_end": 200, "reference_start": 129, "reference_end": 328, "reference_sequence": "MQPNYYRVIKKATSFSGLDMISHAYGQIAGVEVVGFYLWLITEANAQAFNSEIRTPISRLQNAFNTTGTKNVNVPDWIYKTIGKLESLGLVRTFFSQEKSEMTFWIVEPLGWKEFNQKKHFKEKLIESMGKLEYDRNCLSFEQIDNIQFDNALEITANFEANFTSKQDCLSFSFDFEAFHKQLVKQNLFVSFNQKTKAVINGYFEKYQITLEGILECVIPSVVNDEVDLELLQKLLEQLVKNNTAPIVDTVINDRNFFYDNQNLATETKDAISRCHLEYNAEKYLFLLYGKVENEQLDLIRRLRQEFGIADKVINLIVDFSFWKNNSMWREQYILKIAESVERYRSHNNYQATLDNFIRASSLAKKQRTKTKIEKSEPETSAAPVNETDDVNYFLNRIKAINKKNRENGKHKR" }, "paralog": false, "identity": 0.9844155844155844, "score": 743.0, "evalue": 2.33e-275, "description": "Internal stop codon at 641442, 641556, 641640, 641661, 642312. Nonsense mutation around 641442, 641556, 641640, 641661, 642312.", "psc": { "uniref90_id": "UniRef90_P75253", "gene": "DnaB2", "product": "Uncharacterized protein MG349 homolog", "uniref50_id": "UniRef50_P75253", "cog_id": "COG3611", "cog_category": "L", "kegg_orthology_id": "K03346", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75253", "UniRef:UniRef50_P75253", "COG:COG3611", "COG:L", "KEGG:K03346" ] }, "pscc": { "uniref50_id": "UniRef50_P75253", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75253" ], "product": "Uncharacterized protein MG349 homolog" } }, "id": "CNOCLNGIAL_1141", "locus": "GCA000143945_01139" }, { "type": "cds", "sequence": "contig_1", "start": 642550, "stop": 642852, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACACAAACGCTAAAACTCAACCAGCAATAAATGAGTTAGATAAGACTACAGTTCAAGACACAATTAAGAATTACGAACAACAACTTCAAAAAGAGCTTAATGAAGAACAACTAAAAGCTTTTAAAAAGCGCTTTAAGTATTTGAAGCTAACTAAAAGAGAAGTGATTGAAAAGGAAAGCAGGGAACTTTTATTGCAAGCATTTAGAGACTTTGAAAATTGTGAAAAGATAAAAGGCAATCATTGTCCTAATAAAGGCAATGCTCACCTCAACATCTTTCGCGAGCCACCTTTTTGA", "aa": "MENTNAKTQPAINELDKTTVQDTIKNYEQQLQKELNEEQLKAFKKRFKYLKLTKREVIEKESRELLLQAFRDFENCEKIKGNHCPNKGNAHLNIFREPPF", "aa_hexdigest": "e74725482ce126c15413da07ea46fdf6", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11923.459799999984, "isoelectric_point": 8.885951423645022 }, "id": "CNOCLNGIAL_1142", "locus": "GCA000143945_01140" }, { "type": "tRNA", "sequence": "contig_1", "start": 643596, "stop": 643677, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 59.5, "nt": "CCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 643629, 643631 ], "id": "CNOCLNGIAL_16", "locus": "GCA000143945_01141" }, { "type": "cds", "sequence": "contig_1", "start": 643815, "stop": 644159, "strand": "-", "frame": 1, "gene": null, "product": "MPN527 family putative ECF transporter permease subunit", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q9ZB72", "UniRef:UniRef90_P75251" ], "nt": "GTGAGTTTTACACGGTTGTACTTTCACATTTTTGCTCAAATTAAACTTCGTTGGTTATGGGTATTTAGTTTGGCTACTCTATCTAACACTTTGCTGTTAACAACGCTCAACGGTTTGTTAATTACCCCTTTATACTGGTATTGGTTTGGCTATGTGCCGACTGCTAACTTTGTCGAAGTGGCCAAGATATATAACAAAACACCATATTTTCACTTCTTTTTATTTGGCGTACCTAATTATTGGGTTGGCATCTTTGCTTTATACTCACTCTTTAATGTGATTAAATTCACACTAGTGAGTTTAATTGGTGTTCCAGTGATGCGTGCTTTCCAAAAGTTTTACTGA", "aa": "MSFTRLYFHIFAQIKLRWLWVFSLATLSNTLLLTTLNGLLITPLYWYWFGYVPTANFVEVAKIYNKTPYFHFFLFGVPNYWVGIFALYSLFNVIKFTLVSLIGVPVMRAFQKFY", "aa_hexdigest": "f71017177fd79b10087fa79c60faa8cb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 643791, "stop": 644459, "observations": { "mutation": [ 643817, 644261 ], "stop": [ 643817, 644261 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75251", "query_cov": 0.9912280701754386, "subject_cov": 0.5022222222222222, "identity": 0.991, "score": 226.0, "evalue": 1.26e-73, "gene_start": 2, "gene_end": 114, "reference_start": 105, "reference_end": 217, "reference_sequence": "MNGARIAFWPKKEQHQLFNLSFSAMMLALALIASFVSHFISIPFLSALKLTIDISSVFLIACAFFVSYSWALVITVALSLCSFIWDGNNWIGILTLTIANFAIVSFTRLYFHIFAQIKLRWLWVFSLATLSNTLLLTTLNGLLITPLYWYWFGYVPTANFVEVAKIYNKTPYFHFFLFGVPNYWGGIFALYSLFNVIKFTLVSLIGVPVMRAFQKFYWKKAQIVY" }, "paralog": false, "identity": 0.9864864864864865, "score": 439.0, "evalue": 1.17e-159, "description": "Internal stop codon at 643817, 644261. Nonsense mutation around 643817, 644261.", "psc": { "uniref90_id": "UniRef90_P75251", "product": "MPN527 family putative ECF transporter permease subunit", "uniref50_id": "UniRef50_Q9ZB72", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75251", "UniRef:UniRef50_Q9ZB72", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB72" ], "product": "Uncharacterized protein MG350.1" } }, "id": "CNOCLNGIAL_1143", "locus": "GCA000143945_01142" }, { "type": "cds", "sequence": "contig_1", "start": 644259, "stop": 644468, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGCAAGAATAGCTTTTTGGCCAAAAAAAGAACAACACCAACTGTTTAACCTTAGTTTTAGTGCCATGATGCTAGCGCTTGCACTAATTGCTTCGTTTGTTAGTCACTTTATCAGTATTCCGTTTTTGTCAGCACTTAAATTAACGATTGACATTTCATCAGTCTTTTTAATTGCTTGTGCTTTTTTTGTTTCTTATTCTTGA", "aa": "MNGARIAFWPKKEQHQLFNLSFSAMMLALALIASFVSHFISIPFLSALKLTIDISSVFLIACAFFVSYS", "aa_hexdigest": "550e5d77e2c6ceb6ad32534136d2f50c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7703.113999999996, "isoelectric_point": 9.1023717880249 }, "id": "CNOCLNGIAL_1144", "locus": "GCA000143945_01143" }, { "type": "cds", "sequence": "contig_1", "start": 644523, "stop": 645077, "strand": "+", "frame": 3, "gene": "ppa", "product": "Inorganic pyrophosphatase", "db_xrefs": [ "COG:C", "COG:COG0221", "EC:3.6.1.1", "GO:0000287", "GO:0004427", "GO:0005737", "GO:0006796", "RefSeq:WP_014325585.1", "SO:0001217", "UniParc:UPI0001DD6421", "UniRef:UniRef100_A0AAV5N8E4", "UniRef:UniRef50_P47593", "UniRef:UniRef90_P75250" ], "nt": "ATGGACAAATTTTTAATCGATGTTACTGTAGAAATTCCTAAGAGCAGTAAGATCAAGTATGAATACGACCGCAAGACTAGTCAAATCCGTGTCGACAGGATTCTCTTTGGTAGTGAATCTTATCCACAAAACTATGGTTTTATCGCTAACACCTTAGACTGGGATGGTGATGAATTGGACTGTTTTATCTTCGCTGACCAAGCCTTTCTACCAGGTGTAGTAGTACCTACTAGAATTGTAGGAGCTTTAGAAATGGTAGATGACGGTGAATTGGATACCAAACTTTTAGGGGTCATTGATTGTGATCCGCGTTACAAAGAGATTAACTCCGTCAATGATTTACCAAAACACCGCGTTGATGAAATTATTGGTTTTTTCAAAACTTACAAATTACTACAAAAGAAAGAAGTAATTATTAAGGGAGTGCAAAGTTTGGAGTGGGCTAAGAAAGAATACCAAGTGTGTGTTGATTTAATGAAGCAATATGGTAAGTTGCCTAAGGATGAGTTTATCGCTAAAATGCAAAAACTCCATCCTGAACACTACCAAAAATAG", "aa": "MDKFLIDVTVEIPKSSKIKYEYDRKTSQIRVDRILFGSESYPQNYGFIANTLDWDGDELDCFIFADQAFLPGVVVPTRIVGALEMVDDGELDTKLLGVIDCDPRYKEINSVNDLPKHRVDEIIGFFKTYKLLQKKEVIIKGVQSLEWAKKEYQVCVDLMKQYGKLPKDEFIAKMQKLHPEHYQK", "aa_hexdigest": "ce32441bc6e16493b50aa434ceca1d07", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI0001DD6421", "ncbi_nrp_id": "WP_014325585.1", "uniref100_id": "UniRef100_A0AAV5N8E4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6421", "RefSeq:WP_014325585.1", "UniRef:UniRef100_A0AAV5N8E4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N8E4", "uniref90_id": "UniRef90_P75250" }, "psc": { "uniref90_id": "UniRef90_P75250", "gene": "ppa", "product": "Inorganic pyrophosphatase", "ec_ids": [ "3.6.1.1" ], "uniref50_id": "UniRef50_P47593", "cog_id": "COG0221", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0004427", "GO:0000287", "GO:0006796" ] }, "pscc": { "uniref50_id": "UniRef50_P47593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47593" ], "product": "Inorganic pyrophosphatase" }, "genes": [ "ppa" ], "id": "CNOCLNGIAL_1145", "locus": "GCA000143945_01144" }, { "type": "cds", "sequence": "contig_1", "start": 645221, "stop": 645604, "strand": "+", "frame": 2, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "EC:3.1.21.10", "GO:0006281", "GO:0006310", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_Q49422", "UniRef:UniRef90_E2I9M6" ], "nt": "GTGCAAGGAGCATTAACAGCGCAAAAGAAATCGAAAACTGACTATTACGGTGTTTATAAAGGCGAAATTTACTTTGACTTTGAAACTAAACAAACTAATAAAAGTAATTTTGCTCTAGCGCAGTTAGCAGAACACCAACTCAAACACCTTAAAAGAATTAATGAGATTGGTGGTATTAGCTTTTTGTTAATCTATTTTCAGGTACAAGACCTCTTATATGCGTTGCATACCAAGGATCTCTTGCAGATGATCGAAGCGCAAAAGGGTAAAAGTAAAACAATTAAGCGTGACCTTATTGATCAAAAAGCACAAAAGATAAATCTAATCTACCCAGGGGTTATAGATCTAATTGATGTAATAGAAAACTTTATAAAAGACTCTTAG", "aa": "MQGALTAQKKSKTDYYGVYKGEIYFDFETKQTNKSNFALAQLAEHQLKHLKRINEIGGISFLLIYFQVQDLLYALHTKDLLQMIEAQKGKSKTIKRDLIDQKAQKINLIYPGVIDLIDVIENFIKDS", "aa_hexdigest": "f7300cb1a6b5d99f6a2526c9b2b1d623", "start_type": "GTG", "rbs_motif": null, "genes": [ "recU" ], "truncated": "5-prime", "pseudogene": { "start": 645086, "stop": 645604, "observations": { "mutation": [ 645167 ], "stop": [ 645167 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_E2I9M6", "query_cov": 1.0, "subject_cov": 0.7383720930232558, "identity": 0.9840000000000001, "score": 244.0, "evalue": 2.61e-81, "gene_start": 1, "gene_end": 127, "reference_start": 46, "reference_end": 172, "reference_sequence": "MISANRGMFLETIVNQTIVRLGDHPDIWLEKRYLPIQPTAFKETRVQGALTAQKKSKTDYYGVYKGEIYFDFETKQTNKSNFALAQLAEHQLKHLKRINEIGGISFLLIYFQVQDLLYALDTKDLLQMIEAQKGKSKTIKRDLIDQKAQKINLIYPGVIDLIDVIENFIKDS" }, "paralog": false, "identity": 0.9883720930232558, "score": 332.0, "evalue": 2.9e-118, "description": "Internal stop codon at 645167. Nonsense mutation around 645167.", "psc": { "uniref90_id": "UniRef90_E2I9M6", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_Q49422", "kegg_orthology_id": "K03700", "go_ids": [ "GO:0006281", "GO:0006310" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_E2I9M6", "EC:3.1.21.10", "UniRef:UniRef50_Q49422", "KEGG:K03700", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_Q49422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49422" ], "product": "Holliday junction resolvase RecU" } }, "id": "CNOCLNGIAL_1146", "locus": "GCA000143945_01145" }, { "type": "cds", "sequence": "contig_1", "start": 645616, "stop": 645930, "strand": "-", "frame": 3, "gene": "hupA", "product": "putative MG353-like protein", "db_xrefs": [ "COG:B", "COG:COG0776", "GO:0003677", "GO:0030527", "SO:0001217", "UniRef:UniRef50_P47595", "UniRef:UniRef90_P47595" ], "nt": "ATGGAAAAAACAACAACATCGAGTAAGCCATTGTCTCGTAGCGAAATTAACAAGATTATTGCAGTAGCTACTGGAGTAAAAGAGGCTAAGATAAAAGAGATCTTTAAATACCTCAACACCCTCTTACTTAATGAATTAGTAAGTCGTAGCGTTTGCATTTTGCCCGAAAATTTGGGTAAATTACGCATTACGATTCGTAACGCTAGAATTCAAAAAGACATGAAAACTGGTGAAATGAAACACATTCCACCAAAACCATTAGTGCGTTACAGTCCAAGCAAAACCATTAAGGAAACGGCTGCTAAGGTACGCTGA", "aa": "MEKTTTSSKPLSRSEINKIIAVATGVKEAKIKEIFKYLNTLLLNELVSRSVCILPENLGKLRITIRNARIQKDMKTGEMKHIPPKPLVRYSPSKTIKETAAKVR", "aa_hexdigest": "3b0cdc9dd4db5fa729fc2ead1a810834", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47595", "query_cov": 1.0, "subject_cov": 0.9541284403669725, "identity": 0.9129999999999999, "score": 178.0, "evalue": 2.14e-56, "valid": true, "gene": "HupA", "product": "Uncharacterized protein MG353 homolog", "uniref50_id": "UniRef50_P47595", "cog_id": "COG0776", "cog_category": "B", "go_ids": [ "GO:0003677", "GO:0030527" ] }, "pscc": { "uniref50_id": "UniRef50_P47595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47595" ], "product": "Uncharacterized protein MG353" }, "genes": [ "hupA" ], "id": "CNOCLNGIAL_1147", "locus": "GCA000143945_01146" }, { "type": "cds", "sequence": "contig_1", "start": 646079, "stop": 646489, "strand": "+", "frame": 2, "gene": null, "product": "putative MG354-like protein", "db_xrefs": [ "RefSeq:WP_010874887.1", "SO:0001217", "UniParc:UPI0000139690", "UniRef:UniRef100_P75248", "UniRef:UniRef50_P47596", "UniRef:UniRef90_P75248" ], "nt": "ATGGAACCAAATAATTTAAAAGAAGAGCTAGTTTCAGTCTTTGAAAAAGCTTGTTCTTCACATAAAGAAAGGCTTGATTTTATCTGTTCTGTAAGAGAAAGCGATACCTTTTCCAATGTGGATGTACCGCTAGCTCCTATTAAAACTATTATCGAAATTGCTAAAAACGAGGAAAATCAAACCGAAATTCTCAAATTAGCGATCGAAAACATTAAGACTCTATCAACTGTTGGGTCTGGTCAATACATTGCATCGCACTTCTCAACCCATAACGAAGTTGCTATTATTTTCTGTATCTCTTACTTTTTGTATCACTTCAACTTCTTGCACGATGAAAACAAAAAGCAGTTGCTAAAACGTGCCTTTGAAGCAGTTGCTGAAAAAATAGCTGACTACTTAAACGAAAACTAA", "aa": "MEPNNLKEELVSVFEKACSSHKERLDFICSVRESDTFSNVDVPLAPIKTIIEIAKNEENQTEILKLAIENIKTLSTVGSGQYIASHFSTHNEVAIIFCISYFLYHFNFLHDENKKQLLKRAFEAVAEKIADYLNEN", "aa_hexdigest": "3b4796a373f6743d4de66b1311841eeb", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0000139690", "ncbi_nrp_id": "WP_010874887.1", "uniref100_id": "UniRef100_P75248", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139690", "RefSeq:WP_010874887.1", "UniRef:UniRef100_P75248" ] }, "ips": { "uniref100_id": "UniRef100_P75248", "uniref90_id": "UniRef90_P75248" }, "psc": { "uniref90_id": "UniRef90_P75248", "product": "Uncharacterized protein MG354 homolog", "uniref50_id": "UniRef50_P47596" }, "pscc": { "uniref50_id": "UniRef50_P47596", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47596" ], "product": "Uncharacterized protein MG354" }, "genes": [], "id": "CNOCLNGIAL_1148", "locus": "GCA000143945_01147" }, { "type": "cds", "sequence": "contig_1", "start": 646486, "stop": 647610, "strand": "-", "frame": 3, "gene": "clpB", "product": "Chaperone protein ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "GO:0005524", "GO:0005737", "GO:0016887", "GO:0034605", "SO:0001217", "UniRef:UniRef50_P47597", "UniRef:UniRef90_P75247" ], "nt": "TTGCAAACACGCTTAGAGCTCTACCAAACGGAAGGTAACTATGAAGCTGCTTCTAAGATTCTTTACTATGACATCCCCAAACTTAAAAACCAATTAGAACAAGCACAGAAAAAGTATGTTGATTCCAAACATGACTTGTTTAAGACAGAAGTGTCAGAAAACGAAGTGGCCGAAGTAGTATCACAAGCTACCGGTATTCCCTTAAAGAAGCTGTTAGAAACCGAAAAGGAAAAGCTTTTGCATTTAGGTGATGAAATAAAAAAACGGGTTAAGGGTCAAGATGCGGCCGTGGAGACAGTGGTCAATACCGTTATGCGTGGAAGGGTAAACCTCAATGACCCTAACCGGCCAATTGGTTCCTTTATCTTTTTAGGCTCTACTGGTGTTGGTAAAACGGAATTGGCTAAATCACTAGCAGAGGTCTTGTTTGATAATGAAAAAGCGATGATTCGCTTTGACATGAGTGAATACATGGAAAAGCATTCGGTCGCTAAGTTAATCGGAGCGCCTCCTGGATATGTAGGTTATGAACAATCTGGTTTACTAACTGAAGCAGTCCGACGCAAACCTTACTGTGTGCTTCTGTTCGATGAAATTGAAAAAGCACACCCTGATGTAACTAATATTCTTTTACAAGTATTAGATGATGGTACTTTAAAGGACTCTCAAGGTCGTTTAGTTAACTTTAAGAATACGATGATAATTATGACTTCCAACTTGGGTTCCAATTACATTATGGAAAACAAACGCGATTTAGCGATGGAAGCACTAAAAAAACACTTCCGTGCTGAATTTATTAACCGGATTGATGAAATCGTTTTCTTTAGCGTATTGCAAAAAACAACCGTTTTAGAAATTATTACAAATTTATTGGATCAATTAAATCAAAGATTAGCTAAACAAAACTTAAAGTTCACGTTTGACCCTAAGTTAAATGAGTTTATTTACAAATCTAGTTTCGACGAACAGTTTGGTGCTCGACCAATTAAACGTTTTATCGATCGGCAAATAGCTACTCTAATTGCCAAGCAAATTCTGGAAGGTATTATTACTAAAGATGTTAGTTACAACGTAATAGTTGAGAAAGATAAAGTGGCAATAGTAGCCAATAAGGTTAAATCTTAG", "aa": "MQTRLELYQTEGNYEAASKILYYDIPKLKNQLEQAQKKYVDSKHDLFKTEVSENEVAEVVSQATGIPLKKLLETEKEKLLHLGDEIKKRVKGQDAAVETVVNTVMRGRVNLNDPNRPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKAMIRFDMSEYMEKHSVAKLIGAPPGYVGYEQSGLLTEAVRRKPYCVLLFDEIEKAHPDVTNILLQVLDDGTLKDSQGRLVNFKNTMIIMTSNLGSNYIMENKRDLAMEALKKHFRAEFINRIDEIVFFSVLQKTTVLEIITNLLDQLNQRLAKQNLKFTFDPKLNEFIYKSSFDEQFGARPIKRFIDRQIATLIAKQILEGIITKDVSYNVIVEKDKVAIVANKVKS", "aa_hexdigest": "e8ac366f582e392158e748bda5bea9c0", "start_type": "TTG", "rbs_motif": "AAAAA", "psc": { "uniref90_id": "UniRef90_UPI00258F0CA4", "query_cov": 0.9545454545454546, "subject_cov": 0.8419811320754716, "identity": 0.535, "score": 362.0, "evalue": 5.07e-120, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A3D0HNG7" }, "genes": [ "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 646486, "stop": 647910, "observations": { "mutation": [ 647670 ], "stop": [ 647670 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75247", "query_cov": 1.0, "subject_cov": 0.5223463687150838, "identity": 0.997, "score": 709.0, "evalue": 3.17e-252, "gene_start": 1, "gene_end": 374, "reference_start": 343, "reference_end": 716, "reference_sequence": "MDFSFTPTPDKRDFLKEMGRSINDEVLKNKVDPIIGRDNEIRRLIEILSRKNKNNPVLIGEPGVGKTAIVEGFVRRVVNNDVPLNLRDVEIYELSLSGLIAGTQYQGEFEKRVNGILKQVKESNGKIILFIDEIHQIVGLGRNXSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYVEKDGALERRFQKILVSEPSQQEALTIMRGLKTRWELFHNLTIFDSALVAAVEMSARYIPDRNLPDKAIDLIDEASAKIKTEMASEPVVIDTLKREIINLETEYAALKQDKENADNKKKQGHLDNLKQQLDELKKKRDSLTAEWKKEKTNFESINKLKKEIEDLQTRLELYQTEGNYEAASKILYYDIPKLKNQLEQAQKKYVDSKHDLFKTEVSENEVAEVVSQATGIPLKKLLETEKEKLLHLGDEIKKRVKGQDAAVETVVNTVMRGRVNLNDPNRPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKAMIRFDMSEYMEKHSVAKLIGAPPGYVGYEQSGLLTEAVRRKPYCVLLFDEIEKAHPDVTNILLQVLDDGTLKDSQGRLVNFKNTMIIMTSNLGSNYIMENKRDLAMEALKKHFRAEFINRIDEIVFFSVLQKTTVLEIITNLLDQLNQRLAKQNLKFTFDPKLNEFIYKSSFDEQFGARPIKRFIDRQIATLIAKQILEGIITKDVSYNVIVEKDKVAIVANKVKS" }, "paralog": false, "identity": 0.9957805907172996, "score": 909.0, "evalue": 0.0, "description": "Internal stop codon at 647670. Nonsense mutation around 647670.", "psc": { "uniref90_id": "UniRef90_P75247", "gene": "clpB", "product": "Chaperone protein ClpB", "uniref50_id": "UniRef50_P47597", "cog_id": "COG0542", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75247", "UniRef:UniRef50_P47597", "COG:COG0542", "COG:O", "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ] }, "pscc": { "uniref50_id": "UniRef50_P47597", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47597" ], "product": "Chaperone protein ClpB" } }, "id": "CNOCLNGIAL_1149", "locus": "GCA000143945_01148" }, { "type": "cds", "sequence": "contig_1", "start": 647668, "stop": 648633, "strand": "-", "frame": 3, "gene": "clpB", "product": "Chaperone protein ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "GO:0005524", "GO:0005737", "GO:0016887", "GO:0034605", "SO:0001217", "UniRef:UniRef50_P47597", "UniRef:UniRef90_P75247" ], "nt": "ATGGATTTTAGTTTTACACCAACACCAGATAAGCGTGATTTCTTAAAGGAAATGGGGCGTAGCATTAACGATGAGGTGCTCAAAAATAAGGTTGATCCTATTATTGGTCGCGACAACGAAATTCGACGCTTAATTGAAATTTTAAGCCGGAAAAACAAGAACAATCCCGTTTTAATTGGTGAACCAGGCGTCGGGAAAACCGCCATTGTTGAAGGATTTGTAAGAAGGGTAGTTAATAATGACGTGCCTTTAAACTTGCGTGATGTTGAAATTTATGAACTTTCGTTGTCTGGTTTAATTGCTGGCACCCAATACCAAGGTGAGTTTGAAAAACGGGTTAACGGTATCTTAAAGCAAGTCAAAGAATCAAATGGCAAGATTATTTTATTCATTGATGAAATCCACCAAATAGTGGGGCTAGGTCGTAACTCCAGTAGTGGTGCCATGGACATTGCCAACATCTTAAAACCAATGTTAGCACGCGGTGAAATTAAGGTTATTGGTGCCACGACACTGAAAGAGTACCGTGAATATGTGGAGAAGGACGGCGCTTTAGAACGGCGCTTCCAGAAGATCCTTGTCAGTGAACCGAGTCAACAAGAAGCACTTACCATTATGCGGGGTTTGAAGACCCGTTGGGAACTTTTTCATAACCTCACCATCTTTGACAGTGCCTTGGTAGCTGCTGTAGAAATGTCAGCACGTTATATTCCTGATCGTAATTTACCCGATAAAGCGATTGATTTAATTGATGAAGCTAGTGCCAAGATTAAAACGGAAATGGCTTCAGAGCCAGTGGTAATTGACACCCTTAAGCGGGAAATTATTAACTTAGAAACTGAATATGCCGCTTTAAAACAAGATAAGGAAAATGCTGATAACAAGAAAAAACAAGAACACTTAGACAATCTAAAACAACAGTTAGATGAGTTAAAGAAAAAGCGTGATTCTCTAACTGCCGAATGA", "aa": "MDFSFTPTPDKRDFLKEMGRSINDEVLKNKVDPIIGRDNEIRRLIEILSRKNKNNPVLIGEPGVGKTAIVEGFVRRVVNNDVPLNLRDVEIYELSLSGLIAGTQYQGEFEKRVNGILKQVKESNGKIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYVEKDGALERRFQKILVSEPSQQEALTIMRGLKTRWELFHNLTIFDSALVAAVEMSARYIPDRNLPDKAIDLIDEASAKIKTEMASEPVVIDTLKREIINLETEYAALKQDKENADNKKKQEHLDNLKQQLDELKKKRDSLTAE", "aa_hexdigest": "9470523dff3ac16744684dd38a509faf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_U4KPM2", "query_cov": 0.8535825545171339, "subject_cov": 0.9415807560137457, "identity": 0.588, "score": 314.0, "evalue": 1.1e-103, "valid": false, "product": "ATP-dependent Clp protease (ATP-binding subunit, clpB), 1", "uniref50_id": "UniRef50_A0A930RWV4" }, "genes": [ "clpB" ], "truncated": "5-prime", "pseudogene": { "start": 647365, "stop": 648633, "observations": { "mutation": [ 647670 ], "stop": [ 647670 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75247", "query_cov": 1.0031152647975077, "subject_cov": 0.44972067039106145, "identity": 0.9940000000000001, "score": 599.0, "evalue": 6.63e-210, "gene_start": 1, "gene_end": 321, "reference_start": 1, "reference_end": 322, "reference_sequence": "MDFSFTPTPDKRDFLKEMGRSINDEVLKNKVDPIIGRDNEIRRLIEILSRKNKNNPVLIGEPGVGKTAIVEGFVRRVVNNDVPLNLRDVEIYELSLSGLIAGTQYQGEFEKRVNGILKQVKESNGKIILFIDEIHQIVGLGRNXSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYVEKDGALERRFQKILVSEPSQQEALTIMRGLKTRWELFHNLTIFDSALVAAVEMSARYIPDRNLPDKAIDLIDEASAKIKTEMASEPVVIDTLKREIINLETEYAALKQDKENADNKKKQGHLDNLKQQLDELKKKRDSLTAEWKKEKTNFESINKLKKEIEDLQTRLELYQTEGNYEAASKILYYDIPKLKNQLEQAQKKYVDSKHDLFKTEVSENEVAEVVSQATGIPLKKLLETEKEKLLHLGDEIKKRVKGQDAAVETVVNTVMRGRVNLNDPNRPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKAMIRFDMSEYMEKHSVAKLIGAPPGYVGYEQSGLLTEAVRRKPYCVLLFDEIEKAHPDVTNILLQVLDDGTLKDSQGRLVNFKNTMIIMTSNLGSNYIMENKRDLAMEALKKHFRAEFINRIDEIVFFSVLQKTTVLEIITNLLDQLNQRLAKQNLKFTFDPKLNEFIYKSSFDEQFGARPIKRFIDRQIATLIAKQILEGIITKDVSYNVIVEKDKVAIVANKVKS" }, "paralog": false, "identity": 0.9929078014184397, "score": 805.0, "evalue": 2.88e-293, "description": "Internal stop codon at 647670. Nonsense mutation around 647670.", "psc": { "uniref90_id": "UniRef90_P75247", "gene": "clpB", "product": "Chaperone protein ClpB", "uniref50_id": "UniRef50_P47597", "cog_id": "COG0542", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75247", "UniRef:UniRef50_P47597", "COG:COG0542", "COG:O", "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ] }, "pscc": { "uniref50_id": "UniRef50_P47597", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47597" ], "product": "Chaperone protein ClpB" } }, "id": "CNOCLNGIAL_1150", "locus": "GCA000143945_01149" }, { "type": "cds", "sequence": "contig_1", "start": 648809, "stop": 648928, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCAAACACAGCAAATTATTGATTACAAAAAGTGGGTGCAAAAGCGCTTGAACTACTTTTTAAAGGCACTGTTCCCTAATACTTGGAAGATTGCCAAAACAATTCCGTTGTACTAG", "aa": "MPQTQQIIDYKKWVQKRLNYFLKALFPNTWKIAKTIPLY", "aa_hexdigest": "84e24c4ca8c6d5540c98f8f0f6fabad4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4827.7321, "isoelectric_point": 10.09466915130615 }, "id": "CNOCLNGIAL_1151", "locus": "GCA000143945_01150" }, { "type": "cds", "sequence": "contig_1", "start": 648938, "stop": 649210, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01633.27" ], "nt": "GTGGAATTAACAGATAAACACAAAGCGATTCCCAAAACCTTGTGTCACCACGATTCCACCTTTGATAATTTGGTTTATACCCCAAAAAAGCAGGTCGTTTTAATTGACTTTGAGTGGTCTTGTGTGGACAACCCTTACTATGAGATTGCCAACATTATTCGCGAAGAGTTGACAATTGAAACAGCCAACCAAATTATTGACTTGTACGGTAGCTTAGACAAAAAACTAGTTTTTGAAACGGTAATTTACGTGTTGCTGTTTGCCTTTCAGTGA", "aa": "MELTDKHKAIPKTLCHHDSTFDNLVYTPKKQVVLIDFEWSCVDNPYYEIANIIREELTIETANQIIDLYGSLDKKLVFETVIYVLLFAFQ", "aa_hexdigest": "a90ae292dc3c2d7c044b849a270f7ab7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01633.27", "name": "Choline_kinase", "length": 47, "aa_cov": 0.5111111111111111, "hmm_cov": 1.0, "evalue": 4.554095861327216e-09, "score": 31.452850341796875, "start": 11, "stop": 56 } ], "seq_stats": { "molecular_weight": 10554.03859999999, "isoelectric_point": 4.813148689270019 }, "id": "CNOCLNGIAL_1152", "locus": "GCA000143945_01151" }, { "type": "cds", "sequence": "contig_1", "start": 649298, "stop": 649657, "strand": "-", "frame": 2, "gene": "cotS", "product": "putative MG356-like protein", "db_xrefs": [ "COG:COG0510", "COG:H", "SO:0001217", "UniRef:UniRef50_Q49423", "UniRef:UniRef90_P75246" ], "nt": "ATGAATAAGGGCGATGCAATTGCTTTTCTTTGTGAAAAGTTAAACCTCCAACCAACTGCTGTAGCTCATGTGGAACAAATCCACTCGGGTTTTACTAATTTTTCCTTTTTTACAGAACTGAAAGATGGGAGTAAGTACCAAGTCAGGTTAGCGCGTAAAGATGTACCGTTGAACCGACGCAACGAGCAAATTATCCTGGACTTAGTGACCCCCATCTTTGGTAACCCCTTTGTTTACTTTGATGTAACTACCGGTAATGCCATTAAGAAGTGGATTGAAGGAAAACAACCAAAACGTCCCACAAGGCTGTTTTTAGAACAACTAGTGGCTTCTTTAAAAAAAGTACATGCAGTCGAGTGA", "aa": "MNKGDAIAFLCEKLNLQPTAVAHVEQIHSGFTNFSFFTELKDGSKYQVRLARKDVPLNRRNEQIILDLVTPIFGNPFVYFDVTTGNAIKKWIEGKQPKRPTRLFLEQLVASLKKVHAVE", "aa_hexdigest": "0de0c28ea31076910975993e6ffed7d8", "start_type": "ATG", "rbs_motif": null, "genes": [ "cotS" ], "truncated": "5-prime", "pseudogene": { "start": 648995, "stop": 649657, "observations": { "mutation": [ 649276, 649300 ], "stop": [ 649276, 649300 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75246", "query_cov": 1.0, "subject_cov": 0.4219858156028369, "identity": 0.983, "score": 238.0, "evalue": 3.75e-77, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MNKGDAIDFLCEKLNLQPTAVAHVEQIHSGFTNFSFFTELKDGSKYQVRLARKDVPLNRRNEQIILDLVTPIFGNPFVYFDVTAGNAIKKWIEGKQPKRPTRLFLEQLVASLKKVHAVEWKQFANEIWIFDPLIYFNQTKLPDFYKKLYVELTDKHKAIPKTLCHHDSTFDNLVYTPKKQVVLIDFEWSCVDNPYYEIANIIREELTIETANQIIDLYGSLDKKLVFETVIYVLLFAFQWTEIMPQTQQIIDYKKWVQKRLNYFLKALFPNTWKIAKTIPLY" }, "paralog": false, "identity": 0.9818181818181818, "score": 439.0, "evalue": 1.11e-158, "description": "Internal stop codon at 649276, 649300. Nonsense mutation around 649276, 649300.", "psc": { "uniref90_id": "UniRef90_P75246", "gene": "CotS", "product": "Uncharacterized protein MG356 homolog", "uniref50_id": "UniRef50_Q49423", "cog_id": "COG0510", "cog_category": "H", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75246", "UniRef:UniRef50_Q49423", "COG:COG0510", "COG:H" ] }, "pscc": { "uniref50_id": "UniRef50_Q49423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49423" ], "product": "Uncharacterized protein MG356" } }, "id": "CNOCLNGIAL_1153", "locus": "GCA000143945_01152" }, { "type": "cds", "sequence": "contig_1", "start": 649665, "stop": 650837, "strand": "-", "frame": 1, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "GO:0000287", "GO:0005524", "GO:0005829", "GO:0006083", "GO:0006085", "GO:0008776", "KEGG:K00925", "RefSeq:WP_014325588.1", "SO:0001217", "UniParc:UPI0001DD6424", "UniRef:UniRef100_A0AB38W6H5", "UniRef:UniRef50_P75245", "UniRef:UniRef90_P75245" ], "nt": "ATGAACGACAACAAAATTTTAGTAGTCAATGCTGGCAGTAGCTCGATCAAATTCCAATTGTTTGATTATCACAAAAAAGTCCTAGCTAAAGCCCTTTGTGAACGAATTTTTGTCGATGGTTTTTTCAAACTTGAATTTAACGAACAGAAGGTTGAAGAAAAAGTAGCTTTCCCCGACCACCACGCCGCTGTAACCCACTTTTTAAACACACTTAAAAAGCACAAAATTATCCAGGAACTTAGTGACATTACTCTAGTTGGTCACCGCGTGGTGCAGGGCGCTAACTACTTTAAGGATTCGGTGATCGTAGATGCAGCAGCACTCGCTAAAATTAAGGAATTTATTAAGTTAGCGCCACTGCACAACAAGCCAGAGGCTGATGTGATTGAAATCTTTTTCAAAGAAGTTCCTAGTGCTAAGAATGTGGCAGTCTTTGACACAACCTTTCACACTACCATTCCCCAAGAAAACTACTTGTATGCGGTACCACGTTCATGGGAGCAAAAACACTTAGTACGTCGTTATGGCTTCCATGGTACTTCTTACAAGTTCATTAATAACTATTTAGAAAAGCACTTGAACAAGCAAAACCTGAACCTGATTGTGTGTCACTTGGGTAACGGTGCGAGTGTTTGTGCCATTAAGAATGGTAAGTCATTTAATACTTCAATGGGATTCACCCCGCTAGAAGGTCTTATTATGGGAACCCGTAGTGGTGACCTTGACCCTGCCATTATTGGTTATGTAGCAGAACAGGAAAACATGAGTGCTAGTGATGTGGTCAATGCGCTTAATAAAAAATCAGGGATGTTGGCGTTAACTGGTGCATCGGATATGCGTGATGTTTTCGCTAAACCCCAAGAAAACGCTGTGGCTATCAAAATGTATGTTAACCGGGTAGCGGATTACATTGCTAAATACTTGAACCAATTGGAAGGCAACATTGATGGTTTAGTGTTTACTGGTGGCATTGGCGAGAATGCTAGTGATTGTGTAGAGTTGTTTATTAACGCCGTTAAATCATTAGGCTTTGCGACTGATCTAAAACTGTTTGTCAAGTACGGTGATAGCTGTGTGGTATCCACGCCCCAAAGTAAGTACAAGATTTACCGGGTACGTACTAATGAAGAGTTAATGATTGTGGAAGACTCCATTCGACTAACTCAAAAATAG", "aa": "MNDNKILVVNAGSSSIKFQLFDYHKKVLAKALCERIFVDGFFKLEFNEQKVEEKVAFPDHHAAVTHFLNTLKKHKIIQELSDITLVGHRVVQGANYFKDSVIVDAAALAKIKEFIKLAPLHNKPEADVIEIFFKEVPSAKNVAVFDTTFHTTIPQENYLYAVPRSWEQKHLVRRYGFHGTSYKFINNYLEKHLNKQNLNLIVCHLGNGASVCAIKNGKSFNTSMGFTPLEGLIMGTRSGDLDPAIIGYVAEQENMSASDVVNALNKKSGMLALTGASDMRDVFAKPQENAVAIKMYVNRVADYIAKYLNQLEGNIDGLVFTGGIGENASDCVELFINAVKSLGFATDLKLFVKYGDSCVVSTPQSKYKIYRVRTNEELMIVEDSIRLTQK", "aa_hexdigest": "26ef898ff74f45ad64f2b172e0a811ad", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6424", "ncbi_nrp_id": "WP_014325588.1", "uniref100_id": "UniRef100_A0AB38W6H5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6424", "RefSeq:WP_014325588.1", "UniRef:UniRef100_A0AB38W6H5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6H5", "uniref90_id": "UniRef90_P75245" }, "psc": { "uniref90_id": "UniRef90_P75245", "gene": "ackA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_P75245", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925", "go_ids": [ "GO:0005829", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ] }, "pscc": { "uniref50_id": "UniRef50_P75245", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75245" ], "product": "Acetate kinase" }, "genes": [ "ackA" ], "id": "CNOCLNGIAL_1154", "locus": "GCA000143945_01153" }, { "type": "cds", "sequence": "contig_1", "start": 650906, "stop": 651076, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XFQ7", "UniRef:UniRef90_A0AAN4XFQ7" ], "nt": "ATGAGTGCTAAAACTTCATATATTGTGGCTGCTGTTTCGACAGCTGTAGGTATTATTCTCTACTTTATCTCTTCCCTCATGGGGGTAATTGGTGTAAGTAATGATAAGACCTTAATCATTGTCTTTTTTGTACTAGCTTCAATCATCATTATTAACGCCTGTGTTTTTTGA", "aa": "MSAKTSYIVAAVSTAVGIILYFISSLMGVIGVSNDKTLIIVFFVLASIIIINACVF", "aa_hexdigest": "83b14a31674ea43c546bcd3a635081ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XFQ7", "query_cov": 1.0, "subject_cov": 0.8115942028985508, "identity": 1.0, "score": 94.4, "evalue": 2.28e-24, "valid": true, "uniref50_id": "UniRef50_A0AAN4XFQ7" }, "pscc": { "uniref50_id": "UniRef50_A0AAN4XFQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XFQ7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5899.115099999997, "isoelectric_point": 7.915057563781738 }, "id": "CNOCLNGIAL_1155", "locus": "GCA000143945_01154" }, { "type": "cds", "sequence": "contig_1", "start": 651178, "stop": 651600, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_534", "db_xrefs": [ "RefSeq:WP_010874891.1", "SO:0001217", "UniParc:UPI000013AD23", "UniRef:UniRef100_P75244", "UniRef:UniRef50_P75244", "UniRef:UniRef90_P75244" ], "nt": "ATGAATACTTTTAAAGAAACCCTCTTTTCATTAAAGAAAAGTAATTATTTGTTGAATGAGAGTGCTCAAATTCTCAATGATTGTGTAGTAAATAACAATGAAATCGATGAACGCTTACGCAATTCGATTAAATTTGCCCTTGGCATTATTAATTTAGCTAGTTACTGTCTTAAAAACGAACAAGAACAGCTAGACCATGAACTCAACCATGGTAATGAAGCGCCCTGGGAGTTTAGTTTCAATGAAGCTGAACAACTAATAGAATGCACTGTCAAAAATAACTTTGGCAACGAAAAACTAGTGGATTTAATTTTCCACATTGGTGATGCCATGGAGACTTACCGTACCACTAATATTAAGTTTAGAGTACCCAAGTCTTACTATGATGCCAAACAGCAAATTAGAAAGGTAATTAAAAACTAA", "aa": "MNTFKETLFSLKKSNYLLNESAQILNDCVVNNNEIDERLRNSIKFALGIINLASYCLKNEQEQLDHELNHGNEAPWEFSFNEAEQLIECTVKNNFGNEKLVDLIFHIGDAMETYRTTNIKFRVPKSYYDAKQQIRKVIKN", "aa_hexdigest": "19281e5bfc309493ec36856b74870de5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AD23", "ncbi_nrp_id": "WP_010874891.1", "uniref100_id": "UniRef100_P75244", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AD23", "RefSeq:WP_010874891.1", "UniRef:UniRef100_P75244" ] }, "ips": { "uniref100_id": "UniRef100_P75244", "uniref90_id": "UniRef90_P75244" }, "psc": { "uniref90_id": "UniRef90_P75244", "product": "Uncharacterized protein MPN_534", "uniref50_id": "UniRef50_P75244" }, "pscc": { "uniref50_id": "UniRef50_P75244", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75244" ], "product": "Uncharacterized protein MPN_534" }, "genes": [], "id": "CNOCLNGIAL_1156", "locus": "GCA000143945_01155" }, { "type": "cds", "sequence": "contig_1", "start": 651606, "stop": 652226, "strand": "+", "frame": 3, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518", "RefSeq:WP_010874892.1", "SO:0001217", "UniParc:UPI0000135351", "UniRef:UniRef100_P75243", "UniRef:UniRef50_Q49424", "UniRef:UniRef90_P75243" ], "nt": "ATGATTGCGTCAATTTTTGGAAAAATTACCTTTGTTGGTAAGCGAAAAATTATTGTTGAAGCCAATTGTATTTCCTATTGGTTTAATGTGAAAGAAAACCATAGCTTCGAAAAGAACTTGGAAAAACCGCGCCAGGTCTTCTGTCAGATCATTAAAAGAATGGTTACGAACCAGATCTTGGAAGAGGGCTTTGCCTTCAATACCTTAGAGGAAAAGGAATGGTTTTCGAAGTTCATCGAACTCAATGGCATTGGTAGTAAAACTGCTTTAAACTTGCTCAATAACAACCTCGAGGAAATGCGTGATTATATTAAAAACAGTAACTACCATGCCTTGACTAAGTTAACTGGTTCTAACAGTAAGGTAGCTCGCGCATTGCTGGCACTGGAACTGTATGACCGAGACGATGGTGGTAAACGGATTAAACCCAACACTGCCATGGCCAATGACTATGATGAGATGTTTGACACCTTAAAGTCGTTGGGCTATAAACCCCAAGATATCCAAAACGCTTTGAGCAAAATTGAAATTAAACCTAACTTTGATGTAAGTGAAGTGATTGCTGAAGTGATTAAGTTAATGTCTTTCCAGAACAATGAAGTTACAAATAAAACCGCCTAA", "aa": "MIASIFGKITFVGKRKIIVEANCISYWFNVKENHSFEKNLEKPRQVFCQIIKRMVTNQILEEGFAFNTLEEKEWFSKFIELNGIGSKTALNLLNNNLEEMRDYIKNSNYHALTKLTGSNSKVARALLALELYDRDDGGKRIKPNTAMANDYDEMFDTLKSLGYKPQDIQNALSKIEIKPNFDVSEVIAEVIKLMSFQNNEVTNKTA", "aa_hexdigest": "b50832c041587a46bea8bff4b6db39d6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135351", "ncbi_nrp_id": "WP_010874892.1", "uniref100_id": "UniRef100_P75243", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135351", "RefSeq:WP_010874892.1", "UniRef:UniRef100_P75243" ] }, "ips": { "uniref100_id": "UniRef100_P75243", "uniref90_id": "UniRef90_P75243" }, "psc": { "uniref90_id": "UniRef90_P75243", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q49424", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49424" ], "product": "Holliday junction branch migration complex subunit RuvA" }, "genes": [ "ruvA" ], "id": "CNOCLNGIAL_1157", "locus": "GCA000143945_01156" }, { "type": "cds", "sequence": "contig_1", "start": 652201, "stop": 653124, "strand": "+", "frame": 1, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.-", "EC:3.6.4.12", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "RefSeq:WP_014325589.1", "SO:0001217", "UniParc:UPI0001DD6426", "UniRef:UniRef100_A0AAV5NA15", "UniRef:UniRef50_Q49425", "UniRef:UniRef90_P75242" ], "nt": "ATGAAGTTACAAATAAAACCGCCTAACAATTTCGCTGAGTTTGTCGGTAAACAAGAAATTATTAACCAAATTCAACTCAGCATTAAAGCGTCCCGTATCAATAAGGCGCAGTTGGACCACATTCTTTTATACGGTCCACCTGGCGTTGGTAAAACCACATTAGCTCGCTTAATTGCGAGTGAAATGAACACCAAACTGCAAATTATTCAGGGTGGGCACTTACAGCGACCGAGTGATTTTCTCAACGCCGTTTCCCTCATTAAAAAGGGTGATGTACTGTTTGTGGATGAAATCCATGCAGTAGCACCCAGTGTGATGGAGTTAATGTTTCCCGTGATGGATGATTTTCGCGTCCAAGTCTTAATTGGCAAGGACTTTAATTCCAAGATGGTGGAAATGAAAGTTAATCCGTTTACCTTAATCGGAGCCACCACTCAGTTCGGTAAGATTATCAACCCGCTGGAAGATCGCTTTGGGATGATCCTTAACATTGACTACTACAGTAACCAAGAGATCGAGCGGATTGTAAGCATCTATGGTGAGCAAATGGAGTTGGAATTAAAACCGGAAGAAATTACCCAGATTACCCAACACAGTAAGCAAACCCCGCGGATTGCCATTCGAATTGTCAAGCGTTTGTTTGAACAGAAAATAGTCAACAAAAAGATTGATCTGGCTGCTCTGTTCAAAAGCTTAATGATTTACAAAAATGGTTTACAGAGCATTGATGTGCAATATCTCAAAGCACTCAACGGGCAGTACGAGCCTCAGGGTATTAAGTCAATTTGTTCGATGCTAGGGATTGATAAATCCACTGTCGAAAACAAGATTGAGCCCTTCTTGCTGCGGGAAAACATGATCCAAAAGACCAAGAAAGGGCGGATCATAACCCGCACTGGGCGTAATTATTTAACTAGCTGCTAA", "aa": "MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIASEMNTKLQIIQGGHLQRPSDFLNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQVLIGKDFNSKMVEMKVNPFTLIGATTQFGKIINPLEDRFGMILNIDYYSNQEIERIVSIYGEQMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLAALFKSLMIYKNGLQSIDVQYLKALNGQYEPQGIKSICSMLGIDKSTVENKIEPFLLRENMIQKTKKGRIITRTGRNYLTSC", "aa_hexdigest": "998350c82dbb5f055ce8de0f8252ab90", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6426", "ncbi_nrp_id": "WP_014325589.1", "uniref100_id": "UniRef100_A0AAV5NA15", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6426", "RefSeq:WP_014325589.1", "UniRef:UniRef100_A0AAV5NA15" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NA15", "uniref90_id": "UniRef90_P75242" }, "psc": { "uniref90_id": "UniRef90_P75242", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.-", "3.6.4.12" ], "uniref50_id": "UniRef50_Q49425", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49425" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "CNOCLNGIAL_1158", "locus": "GCA000143945_01157" }, { "type": "cds", "sequence": "contig_1", "start": 653110, "stop": 653772, "strand": "-", "frame": 3, "gene": "dinP", "product": "putative MG360-like protein", "db_xrefs": [ "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0009432", "GO:0042276", "SO:0001217", "UniRef:UniRef50_P75241", "UniRef:UniRef90_P75241" ], "nt": "GTGGAACTTCCTGGGATAGGCAAACGACAACTTGACAACGCCTTTAAAAATAATTTTCACAAGATTGGAGACCTCGCTAAGTGTAAAGATGTGACCTTGTTCAAACGGGTGTTTGGTATTGCGTGGGAAAGCTTGCACGCTGTGGCTTTGGGTGAAACTTATACGCAAAGCGAGCAGGATGTCAAAAGTCGCTCAATTGCTGTAAGTGAAACGCTGGAAGATTTGAACTACTCTTCCAATCAACTCCAGCAAAAACTCACTAGTATTTTCAACGAGTTGTATGCTAGGTTACAACTGTCCTTTCAAATGTGCAAGGGAGTAGTGGTGCAGTTAAAGAGCAATGACTTTATTGTCAACTCCCACTCTCAATCGATCAAGAAATACACCGCTGATTACCAAACCCTACTCGTAATAGTCAAAAAGCTCTTTAACAGGCTATTAATGGGTGTGGGGCTTAACATCCGCTTAATTGGCGTGAGTTTTTTTGGATTAAAAAATAACCCCTCCAGTTCAAGACCAGAAGGGTTACTTTTTTATGAGTATCAGCAAGCTAAGCCTAAACAACAGACTGCTCACTTTGCTTTAGATCAAATGATCTATGAGATTAACCAAAGCTTTGGTTATGAGATTATCCAGCGTGCCAAAAAGTTAGCAGCTAGTTAA", "aa": "MELPGIGKRQLDNAFKNNFHKIGDLAKCKDVTLFKRVFGIAWESLHAVALGETYTQSEQDVKSRSIAVSETLEDLNYSSNQLQQKLTSIFNELYARLQLSFQMCKGVVVQLKSNDFIVNSHSQSIKKYTADYQTLLVIVKKLFNRLLMGVGLNIRLIGVSFFGLKNNPSSSRPEGLLFYEYQQAKPKQQTAHFALDQMIYEINQSFGYEIIQRAKKLAAS", "aa_hexdigest": "90bf47b9eb9554f52e98fc31ac938fa3", "start_type": "GTG", "rbs_motif": null, "genes": [ "dinP" ], "truncated": "3-prime", "pseudogene": { "start": 653110, "stop": 654072, "observations": { "mutation": [ 653787 ], "stop": [ 653787 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75241", "query_cov": 1.0, "subject_cov": 0.5339805825242718, "identity": 0.991, "score": 422.0, "evalue": 2.87e-146, "gene_start": 1, "gene_end": 220, "reference_start": 193, "reference_end": 412, "reference_sequence": "MIGTFTYLDPTIQADPNLLFFYFDFDAFFASVEEIENPELKNQPLIVGNRTSRSVVSTCNYLARSYGIKSGMPIAKALELCPQAIFATSHFRNYRKYSAKIFAMIAEQFNLEVHTLSIDEGFVCFRDLSPRKAFSLAKRIQRHVYEQLNFHISIGISNQFTLAKIFSNQAKPFGVKSCFSKEVKRKLWPLPIVELPGIGKRQLDNAFKNNFHKIGDLAKCKDVTLFKRVFGIAWESLHAVALGETYTQSEQDVKSRSIAVSETLEYLNYSSNQLQQKLTSIFNELYARLQLSFQMCKGVVVQLKSNDFIVNSHSQSIKKYTADYQTLLVIVKKLFNRLLMGVGLNIRLIGVSFFGLKNNPSSSRPEGLLFYEYQQAKPKQQTAHFALDQMIYEINQSFGYEIIQRAKKLAAS" }, "paralog": false, "identity": 0.99375, "score": 623.0, "evalue": 1.14e-227, "description": "Internal stop codon at 653787. Nonsense mutation around 653787.", "psc": { "uniref90_id": "UniRef90_P75241", "gene": "DinP", "product": "Uncharacterized protein MG360 homolog", "uniref50_id": "UniRef50_P75241", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75241", "UniRef:UniRef50_P75241", "COG:COG0389", "COG:L", "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75241", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75241" ], "product": "Uncharacterized protein MG360 homolog" } }, "id": "CNOCLNGIAL_1159", "locus": "GCA000143945_01158" }, { "type": "cds", "sequence": "contig_1", "start": 653785, "stop": 654348, "strand": "-", "frame": 3, "gene": "dinP", "product": "putative MG360-like protein", "db_xrefs": [ "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0009432", "GO:0042276", "SO:0001217", "UniRef:UniRef50_P75241", "UniRef:UniRef90_P75241" ], "nt": "ATGATCGGCACTTTTACATATTTAGATCCAACCATACAAGCTGATCCTAATCTGCTTTTTTTCTATTTTGATTTTGATGCCTTTTTTGCATCAGTAGAAGAGATAGAAAACCCCGAGCTCAAAAATCAACCTTTAATTGTCGGTAACCGGACATCTAGGAGTGTGGTGTCGACTTGTAATTACCTTGCCCGGAGCTATGGCATTAAGTCGGGCATGCCAATTGCTAAGGCCTTGGAATTGTGTCCCCAAGCGATCTTTGCCACATCACACTTTCGTAACTACCGCAAGTATTCCGCTAAGATCTTTGCCATGATTGCTGAGCAGTTTAACCTAGAGGTACACACCCTTTCGATTGATGAGGGCTTTGTGTGTTTTCGTGACCTTTCACCCCGTAAAGCATTTAGTTTGGCTAAACGGATTCAGCGCCACGTTTATGAACAGTTAAACTTTCACATCTCCATTGGTATTTCCAACCAATTTACCCTAGCGAAAATCTTTTCCAATCAGGCTAAACCGTTTGGGGTTAAAAGCTGTTTTAGCAAGGAAGTTAAACGCAAACTCTGA", "aa": "MIGTFTYLDPTIQADPNLLFFYFDFDAFFASVEEIENPELKNQPLIVGNRTSRSVVSTCNYLARSYGIKSGMPIAKALELCPQAIFATSHFRNYRKYSAKIFAMIAEQFNLEVHTLSIDEGFVCFRDLSPRKAFSLAKRIQRHVYEQLNFHISIGISNQFTLAKIFSNQAKPFGVKSCFSKEVKRKL", "aa_hexdigest": "ef81b4fc58cc981941b32eeebdbd9fa7", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "dinP" ], "truncated": "5-prime", "pseudogene": { "start": 653482, "stop": 654348, "observations": { "mutation": [ 653787 ], "stop": [ 653787 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75241", "query_cov": 1.0, "subject_cov": 0.4538834951456311, "identity": 1.0, "score": 374.0, "evalue": 1.04e-127, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MIGTFTYLDPTIQADPNLLFFYFDFDAFFASVEEIENPELKNQPLIVGNRTSRSVVSTCNYLARSYGIKSGMPIAKALELCPQAIFATSHFRNYRKYSAKIFAMIAEQFNLEVHTLSIDEGFVCFRDLSPRKAFSLAKRIQRHVYEQLNFHISIGISNQFTLAKIFSNQAKPFGVKSCFSKEVKRKLWPLPIVELPGIGKRQLDNAFKNNFHKIGDLAKCKDVTLFKRVFGIAWESLHAVALGETYTQSEQDVKSRSIAVSETLEYLNYSSNQLQQKLTSIFNELYARLQLSFQMCKGVVVQLKSNDFIVNSHSQSIKKYTADYQTLLVIVKKLFNRLLMGVGLNIRLIGVSFFGLKNNPSSSRPEGLLFYEYQQAKPKQQTAHFALDQMIYEINQSFGYEIIQRAKKLAAS" }, "paralog": false, "identity": 0.9930555555555556, "score": 567.0, "evalue": 2.56e-206, "description": "Internal stop codon at 653787. Nonsense mutation around 653787.", "psc": { "uniref90_id": "UniRef90_P75241", "gene": "DinP", "product": "Uncharacterized protein MG360 homolog", "uniref50_id": "UniRef50_P75241", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75241", "UniRef:UniRef50_P75241", "COG:COG0389", "COG:L", "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75241", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75241" ], "product": "Uncharacterized protein MG360 homolog" } }, "id": "CNOCLNGIAL_1160", "locus": "GCA000143945_01159" }, { "type": "cds", "sequence": "contig_1", "start": 654466, "stop": 654951, "strand": "+", "frame": 1, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180", "KEGG:K02864", "RefSeq:WP_010874895.1", "SO:0001217", "UniParc:UPI0000133B3C", "UniRef:UniRef100_P75240", "UniRef:UniRef50_P36263", "UniRef:UniRef90_P75240" ], "nt": "ATGGAAGCTAAAAAAGATAAAGCTCAGCAGGTTGCTGATGTCTCCCATTTGCTTTCAACCAGTGCAGGATTTGTCATTTTTGACTATACCAGCATGTCAGCAATTGAAGCGACTTCTATTCGCAAGAAGTTGTTTAAGAATGGCAGCAAAATTAAGGTAATTAAAAACAATATTTTGCGCCGTGCTTTAAAAGCTGGTAAGTTTGAAGGAATTGACGAAACTGCCATTAAAGGCAAATTGGCGGTTGCTGTTGGAGTGAACGAAATTGTAGAAACGTTGAAAGCTGTTGACGGTGTGGTAAAGGCGAAGGAAGCGATGAACTTTGTTTGTGGTTACTTTGACAACCGTGCTTTTAACAGTGCTGATTTAGAGAAGATTGCAAAACTGCCAGGACGAAACGAACTTTATGGGATGTTTCTCTCGGTATTACAAGCTCCATTGCGCAAGTTCTTATACGCTTTGGAAGCAGTTAAGGCTGCAAAATAA", "aa": "MEAKKDKAQQVADVSHLLSTSAGFVIFDYTSMSAIEATSIRKKLFKNGSKIKVIKNNILRRALKAGKFEGIDETAIKGKLAVAVGVNEIVETLKAVDGVVKAKEAMNFVCGYFDNRAFNSADLEKIAKLPGRNELYGMFLSVLQAPLRKFLYALEAVKAAK", "aa_hexdigest": "82648b8a97d4dd48affe0eb54c8d0619", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133B3C", "ncbi_nrp_id": "WP_010874895.1", "uniref100_id": "UniRef100_P75240", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133B3C", "RefSeq:WP_010874895.1", "UniRef:UniRef100_P75240" ] }, "ips": { "uniref100_id": "UniRef100_P75240", "uniref90_id": "UniRef90_P75240" }, "psc": { "uniref90_id": "UniRef90_P75240", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_P36263", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P36263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36263" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "CNOCLNGIAL_1161", "locus": "GCA000143945_01160" }, { "type": "cds", "sequence": "contig_1", "start": 654989, "stop": 655357, "strand": "+", "frame": 2, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_014325591.1", "SO:0001217", "UniParc:UPI0001DD6428", "UniRef:UniRef100_A0AAP8E540", "UniRef:UniRef50_P36255", "UniRef:UniRef90_P36255" ], "nt": "ATGGCAAAACTAGATAAAAACCAATTAATTGAATCGTTGAAGGAAATGACCATCATGGAAATCGATGAAATCATTAAGGCTGTAGAAGAAGCTTTTGGAGTATCGGCAACACCTGTAGTAGCTGCTGGTGCTGTTGGTGGTACACAAGAAGCTGCTAGCGAAGTGACTGTGAAAGTTACTGGTTACGCTGACAACGCTAAATTAGCTGTGTTAAAGCTTTACCGCGAAATTGCTGGTGTTGGTTTAATGGAAGCTAAAACTGCTGTGGAAAAACTTCCTTGTGTTGTTAAGCAAGACATCAAACCTGAAGAAGCTGAAGAACTTAAAAAGCGTTTCGTTGAAGTTGGAGCAACTGTTGAAATCAAATAA", "aa": "MAKLDKNQLIESLKEMTIMEIDEIIKAVEEAFGVSATPVVAAGAVGGTQEAASEVTVKVTGYADNAKLAVLKLYREIAGVGLMEAKTAVEKLPCVVKQDIKPEEAEELKKRFVEVGATVEIK", "aa_hexdigest": "a01200b5df09d6b30a7501353e9cf82c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6428", "ncbi_nrp_id": "WP_014325591.1", "uniref100_id": "UniRef100_A0AAP8E540", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6428", "RefSeq:WP_014325591.1", "UniRef:UniRef100_A0AAP8E540" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E540", "uniref90_id": "UniRef90_P36255" }, "psc": { "uniref90_id": "UniRef90_P36255", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_P36255", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P36255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36255" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "CNOCLNGIAL_1162", "locus": "GCA000143945_01161" }, { "type": "cds", "sequence": "contig_1", "start": 655547, "stop": 655810, "strand": "-", "frame": 2, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02968", "RefSeq:WP_014325593.1", "SO:0001217", "UniParc:UPI0002386BED", "UniRef:UniRef100_A0AAX0S2C1", "UniRef:UniRef50_P55750", "UniRef:UniRef90_P75237" ], "nt": "ATGGCTAATATCAAATCGAACGAAAAACGTTTACGACAAAACATTAAACGTAATCTGAATAACAAGGGACAGAAAACTAAGTTAAAGACCAACGTGAAGAATTTTCACAAGGAAATTAACCTAGACAACCTTGGTAATGTTTATTCCCAAGCAGATCGCTTAGCACGTAAAGGTATTATTTCTACTAATCGTGCGCGTCGTTTAAAATCACGCAACGCTGCTGTTTTAAATAAAACACAAGTAACAGCTGTTGAAGGCAAATAA", "aa": "MANIKSNEKRLRQNIKRNLNNKGQKTKLKTNVKNFHKEINLDNLGNVYSQADRLARKGIISTNRARRLKSRNAAVLNKTQVTAVEGK", "aa_hexdigest": "356901d664cb93989653b6191ea76d9f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386BED", "ncbi_nrp_id": "WP_014325593.1", "uniref100_id": "UniRef100_A0AAX0S2C1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386BED", "RefSeq:WP_014325593.1", "UniRef:UniRef100_A0AAX0S2C1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S2C1", "uniref90_id": "UniRef90_P75237" }, "psc": { "uniref90_id": "UniRef90_P75237", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_P55750", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P55750", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55750" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "CNOCLNGIAL_1163", "locus": "GCA000143945_01162" }, { "type": "cds", "sequence": "contig_1", "start": 655871, "stop": 656029, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGGTGGACAACAGGGTGGTTTTTTTGGCCTACTCGTCATAATTATCCCTGTCATATTGTTGATTGTCTTTTTCTCTAAAAAGAAGAATTCACAAAAAACAGAGTTTGGTGGTGAAGGGGGCAGCCGTGCTTCCAAAAAAGATGAAGTTTGA", "aa": "MNGGGQQGGFFGLLVIIIPVILLIVFFSKKKNSQKTEFGGEGGSRASKKDEV", "aa_hexdigest": "b3b6d63816216fe6ea79769fefa8c627", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5528.383600000002, "isoelectric_point": 9.69799518585205 }, "id": "CNOCLNGIAL_1164", "locus": "GCA000143945_01163" }, { "type": "cds", "sequence": "contig_1", "start": 656114, "stop": 656527, "strand": "+", "frame": 2, "gene": null, "product": "putative MG364-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604", "UniRef:UniRef90_P75236" ], "nt": "TTGCACTCCAGGAAAGACCGGCAGTTTTTTAACCAAGAAGTGCAAGCTTACATTACAGCCCATAACTTAAGCAAAACAGCAGCCAAGCGTTACCGTCACGAGCAATTAAAACTCAAACAACGTGAACTGTACTGCATTTACTTCATTACCAAGGACGCTAAAACCAGCGTTTTTGATGAAGCACGCATTATTGAAGCTGAAGTTTACCAAAAGCCCAATAAAACGGGTAAGGGTGCACCAGAACGTTTAATTCGAATCTTAGGATTGAAAAACTTCAATGACGAAATGAAGTGGATTCAACCACTAATGGATCGCGAGGAAAAACGAAAGGAAAAAGAAGAGAAACGCAAGCGGGAATTGGCAGCACGTCAGTTAAAAAGACAAGAAAAGAAAAAACAAAAAACTTCCAAATAA", "aa": "MHSRKDRQFFNQEVQAYITAHNLSKTAAKRYRHEQLKLKQRELYCIYFITKDAKTSVFDEARIIEAEVYQKPNKTGKGAPERLIRILGLKNFNDEMKWIQPLMDREEKRKEKEEKRKRELAARQLKRQEKKKQKTSK", "aa_hexdigest": "be6a29ee600b559f269b992cea5c13c5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 655970, "stop": 656527, "observations": { "mutation": [ 656027 ], "stop": [ 656027 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75236", "query_cov": 1.0, "subject_cov": 0.6284403669724771, "identity": 0.993, "score": 233.0, "evalue": 9.5e-76, "gene_start": 1, "gene_end": 137, "reference_start": 82, "reference_end": 218, "reference_sequence": "MNGGGQQGGFFGLLVIIIPVILLIVFFSKKKNSQKTEFGGEGGSRASKKDEVWKTVKQFLQEQNERGKEIIKTFVAKNPNPLHSRKDRQFFNQEVQAYITAHNLSKTAAKRYRHEQLKLKQRELYCIYFITKDAKTSVFDEARIIEAEVYQKPNKTGKGAPERLIRILGLKNFNDEMKWIQPLMDREEKRKEKEEKRKRELAARQLKRQEKKKQKTSK" }, "paralog": false, "identity": 0.9837837837837838, "score": 327.0, "evalue": 2.79e-115, "description": "Internal stop codon at 656027. Nonsense mutation around 656027.", "psc": { "uniref90_id": "UniRef90_P75236", "product": "Uncharacterized protein MG364 homolog", "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75236", "UniRef:UniRef50_P47604" ] }, "pscc": { "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604" ], "product": "Uncharacterized protein MG364" } }, "id": "CNOCLNGIAL_1165", "locus": "GCA000143945_01164" }, { "type": "cds", "sequence": "contig_1", "start": 656532, "stop": 656897, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00551.24" ], "nt": "ATGATTAAAGTGGTTTTCTTTGGGACATCCACCCTTTCAAAGTGTTGTTTAGAAGCAATTTTTCAAGATCCTGAGTTTGTGGTTTGCGGCGTAGTGACCCAGCCCGATAAGGTAAACGAGCGCAACAATAAAATTAACTTTTCTGCAGTTAAACAATTTTGTATAGAAAACAACATTCCCTGTTTTCAACCAGAAAAAAACATTCAGATTAAAACAGAGTTAGCCCAACTCCAAGCTGATATTGGCGTTTGTGTAGCCTTTGGCCAATACATCCACAACGATATTATTAACCTCTTTCCTTACAAGATTGCCAACCTACACCCCTCAAAATTACCACTACTGCGTGGTGGAGCACCATTGCACTGA", "aa": "MIKVVFFGTSTLSKCCLEAIFQDPEFVVCGVVTQPDKVNERNNKINFSAVKQFCIENNIPCFQPEKNIQIKTELAQLQADIGVCVAFGQYIHNDIINLFPYKIANLHPSKLPLLRGGAPLH", "aa_hexdigest": "384d25ffad9edeb7870b72daad1e208b", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00551.24", "name": "Formyl_trans_N", "length": 123, "aa_cov": 0.9752066115702479, "hmm_cov": 0.975609756097561, "evalue": 6.617064437996437e-16, "score": 54.031036376953125, "start": 3, "stop": 120 } ], "seq_stats": { "molecular_weight": 13547.68919999998, "isoelectric_point": 7.602692604064942 }, "id": "CNOCLNGIAL_1166", "locus": "GCA000143945_01165" }, { "type": "cds", "sequence": "contig_1", "start": 657285, "stop": 657467, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02911.23" ], "nt": "ATGACAAGTACAACCGCAGTTGGTCAAATCACTAAAATTTCTAAACAAGGTATAGAAATCGCCTTAGCTAATGATGAAATTGTTCTACTCCAAATAATTCAAATTCCGGGAAAGCGGGCAATGGGAGTGTCAGAAATAATTAACGGTAAACATCCTTTTGCTGAAGGTAAATTTTTTCATTAA", "aa": "MTSTTAVGQITKISKQGIEIALANDEIVLLQIIQIPGKRAMGVSEIINGKHPFAEGKFFH", "aa_hexdigest": "19dbfaca56bd86a3e6a12aada593cd65", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 44, "aa_cov": 0.7333333333333333, "hmm_cov": 0.9772727272727273, "evalue": 3.454856337243261e-08, "score": 29.089759826660156, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 6491.536899999999, "isoelectric_point": 8.18195667266846 }, "id": "CNOCLNGIAL_1167", "locus": "GCA000143945_01166" }, { "type": "cds", "sequence": "contig_1", "start": 657467, "stop": 658591, "strand": "+", "frame": 2, "gene": null, "product": "putative MG366-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47606", "UniRef:UniRef50_UPI00031D7E73", "UniRef:UniRef90_P75234" ], "nt": "ATGCTCACCAAGGCCTTTTTTTTAAAAAACTTTGATCGCTCAAAGGAGTTGATTCCTCTGTCTTACGCTGATGTTTTTGGCGCTGCTAGTCAATTGTTAAAAAAACACCATAAGCAGGTCGATACCGAAATTGATGTGCAAGAAGATCTAATTGAAGATCCGGTATTTGAAATAGATATTTTAGAGCTACTCAACGAAGCGCCAGAACTATTATTTGATAGTAAAAATCCTCGGGTTAAAGAGGCACAAATCATTATTGAAAAGGCCAAAAAAGATATTGCTAGCTATTTCCACTTAGACAATATTTTAGACACTGACAACTTAGGGTTAAAAGCCACTGTTACCGAAAAAATCCAATTTACCGAAAAACAAATCGAAGCTGCAGTACAAAACAAAAAAGCAGCGATTATTTTTAAACCAGTGTTTACCGTCAACCAGTGTTTAATCCAACCAGATGCTGTAGTAGTACATGCTAATGGTTTGTGTGAGTTTGTTGTTATTAAAGCGACAACAAATACCAAGAGAAAATTTATCTTGGAAATTATTTATGACTTCTTGCTGTTTGAAAAATTGGGCAAATACAAACTGGTTAACTATTACTTCTGCATAGTTAATTATGAACTGAAAAATAAACACAATGTTTCCTTCTTTTTAAACACCGAAATTAAAACAGCCAAAAACAGTAGTACGAGTAAAACTAAAGAAGAGCAAGTGTTGTACGGGCATCTCCCTTTTAATGATCCAAAAAAAATTGCTTACATTCATAGCAAGAAGAGTGGTGGTGTCAATGGTTTTCTGCTCGTCAAACTAGTGGACAACATTATTAGAAGCGGTGTTACTAATTTGGAACAAATTATTAGTTTTGTTTTTCGTGAACTGAATGCACCCAGCATTCGTAGCTTAAAGCAAATTATTAGTGAAGTAGCTAAAGTACAACTTGATTTTTGGAACATTATTGATGATGTTAAACAACACCAAGAGTTACAAGACAACCAAATTACCTTCAATTACAGTGATGCCTTCAACTCTTTTTGGAATAACTATTTATTGCGCAATCTAATTAAATTAGTCTTTGCTTATAAATACAGTGAAATCTTTCGACTTTCAGGAAAATTAGCTAAATGA", "aa": "MLTKAFFLKNFDRSKELIPLSYADVFGAASQLLKKHHKQVDTEIDVQEDLIEDPVFEIDILELLNEAPELLFDSKNPRVKEAQIIIEKAKKDIASYFHLDNILDTDNLGLKATVTEKIQFTEKQIEAAVQNKKAAIIFKPVFTVNQCLIQPDAVVVHANGLCEFVVIKATTNTKRKFILEIIYDFLLFEKLGKYKLVNYYFCIVNYELKNKHNVSFFLNTEIKTAKNSSTSKTKEEQVLYGHLPFNDPKKIAYIHSKKSGGVNGFLLVKLVDNIIRSGVTNLEQIISFVFRELNAPSIRSLKQIISEVAKVQLDFWNIIDDVKQHQELQDNQITFNYSDAFNSFWNNYLLRNLIKLVFAYKYSEIFRLSGKLAK", "aa_hexdigest": "d7297195fa447fccef86a89f0f9c2dd4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00031D7E73", "query_cov": 0.9973262032085561, "subject_cov": 0.8197802197802198, "identity": 0.627, "score": 470.0, "evalue": 7.74e-162, "valid": false, "uniref50_id": "UniRef50_UPI00031D7E73" }, "pscc": { "uniref50_id": "UniRef50_UPI00031D7E73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031D7E73" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 657467, "stop": 658894, "observations": { "mutation": [ 658589, 658733, 658781 ], "stop": [ 658589, 658733, 658781 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75234", "query_cov": 1.0, "subject_cov": 0.5632530120481928, "identity": 1.0, "score": 721.0, "evalue": 1.44e-257, "gene_start": 1, "gene_end": 374, "reference_start": 1, "reference_end": 374, "reference_sequence": "MLTKAFFLKNFDRSKELIPLSYADVFGAASQLLKKHHKQVDTEIDVQEDLIEDPVFEIDILELLNEAPELLFDSKNPRVKEAQIIIEKAKKDIASYFHLDNILDTDNLGLKATVTEKIQFTEKQIEAAVQNKKAAIIFKPVFTVNQCLIQPDAVVVHANGLCEFVVIKATTNTKRKFILEIIYDFLLFEKLGKYKLVNYYFCIVNYELKNKHNVSFFLNTEIKTAKNSSTSKTKEEQVLYGHLPFNDPKKIAYIHSKKSGGVNGFLLVKLVDNIIRSGVTNLEQIISFVFRELNAPSIRSLKQIISEVAKVQLDFWNIIDDVKQHQELQDNQITFNYSDAFNSFWNNYLLRNLIKLVFAYKYSEIFRLSGKLAKWDEVVEAYKENKSVKIDGFLYELNQGKMKKTKNPATSQFNKIHFFLRAWNDKKGIAVGNKFKSVWQKLKEKKVYFDFETISSAVRVIDKSLPFTQIVTQCSLIVDDNTESDKSKLVCQNLIFDPLTIGIEDFKTVVDALYQKQCDQYSFVVYNKSFEKNRLLEMATFINEAPYQQRVQAIIENLFDLADIFGLENDCLAFKQLDGFSSIKKVLPMIDQRFLDASRTVSYQSLKVQKGDVAQELTLARFLNCLDEQQWAQTALELKQYCENDVRAMIAIELFIKDFITNQL" }, "paralog": false, "identity": 0.991578947368421, "score": 920.0, "evalue": 0.0, "description": "Internal stop codon at 658589, 658733, 658781. Nonsense mutation around 658589, 658733, 658781.", "psc": { "uniref90_id": "UniRef90_P75234", "product": "Uncharacterized protein MG366 homolog", "uniref50_id": "UniRef50_P47606", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75234", "UniRef:UniRef50_P47606" ] }, "pscc": { "uniref50_id": "UniRef50_P47606", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47606" ], "product": "Uncharacterized protein MG366" } }, "id": "CNOCLNGIAL_1168", "locus": "GCA000143945_01167" }, { "type": "cds", "sequence": "contig_1", "start": 658877, "stop": 659359, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "GTGACCCAGTGTTCTCTTATTGTTGATGACAATACCGAGTCTGATAAAAGCAAGTTAGTTTGTCAAAACCTGATCTTTGATCCGCTAACTATTGGTATTGAGGACTTTAAAACAGTCGTTGATGTCCTTTACCAAAAGCAGTGTGACCAGTACAGTTTTGTCGTTTATAACAAATCGTTTGAAAAGAACAGGTTACTGGAAATGGTTACTTTTATCAATGAAGCTCCATACCAACAAAGAGTGCAAGCAATTATTGAAAATTTATTTGATTTAGCAGATATTTTTGGACTTGAAAATGATTGCTTAGCCTTTAAACAACTTGATGGTTTTTCATCAATTAAAAAGGTCTTGCCAATGATTGATCAACGCTTTTTAGATGCTAGTCGAACTGTTAGTTACCAATCATTAAAGGTACAAAAAGGTGATGTAGCACAAGAGCTTACTTTGGCACGCTTTTTAAATTGTTTAGACGAACAGCAATGA", "aa": "MTQCSLIVDDNTESDKSKLVCQNLIFDPLTIGIEDFKTVVDVLYQKQCDQYSFVVYNKSFEKNRLLEMVTFINEAPYQQRVQAIIENLFDLADIFGLENDCLAFKQLDGFSSIKKVLPMIDQRFLDASRTVSYQSLKVQKGDVAQELTLARFLNCLDEQQ", "aa_hexdigest": "6642c7207dff469e5308ac32a11f0aa4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 112, "aa_cov": 0.69375, "hmm_cov": 0.9017857142857143, "evalue": 4.668611681043807e-14, "score": 48.245330810546875, "start": 2, "stop": 112 } ], "seq_stats": { "molecular_weight": 18441.88499999999, "isoelectric_point": 4.52236042022705 }, "id": "CNOCLNGIAL_1169", "locus": "GCA000143945_01168" }, { "type": "cds", "sequence": "contig_1", "start": 659502, "stop": 660122, "strand": "-", "frame": 1, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "COG:COG0571", "COG:K", "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47607", "UniRef:UniRef90_P75233" ], "nt": "TTGGAATTTTTAGGTGATGCCTTAATTGATTTCATAGTAGCGAAAAAACTGTTTGAGCTTTATCCCAATTACAACGAAGGTATGTTGACCAGAACTAAGATTGAAATTGTTAAGGGCGAAAACCTCAACCGTATTGGGAAGGAATTGAACTTTGGCAATTTCATTAAGTTAGGTAAAGGAATGCCGTATACTGAAACACTTTTTGGTGATGTACTAGAGGCGTTAGTGGCAGCCATTTACGAAGACCTTGGCATAGAGAAAGCGAATCAATTTGTCGAAGAACATATTTTTAAAAAAACTTACTCAGAAATTCTCAAGTACAACTTCTTTTCACTGTTTCAAGAACAAAAGCTACCAGAACCTAGGGTACGGGTTAGTTTAACTAGCAATAACCTTGTGTTGAGTATTATTGAGCTTAACGGTGACATTATCTGGTCACAAGCTGTACCTAACAGTAAACACTATGATGACAAGAGCGTGTTAGAACACAATGCAATGTCAGCCTTTACCCAGTTTCTTAAAAGTGGTAAAGGCATTAACTTTTTTAGTGACATTAAAAATAAGTTAGACAGTCAAAAGCCTATGCGAGCTTTAACAGTTAGACCGAAAAAGATTAACTGA", "aa": "MEFLGDALIDFIVAKKLFELYPNYNEGMLTRTKIEIVKGENLNRIGKELNFGNFIKLGKGMPYTETLFGDVLEALVAAIYEDLGIEKANQFVEEHIFKKTYSEILKYNFFSLFQEQKLPEPRVRVSLTSNNLVLSIIELNGDIIWSQAVPNSKHYDDKSVLEHNAMSAFTQFLKSGKGINFFSDIKNKLDSQKPMRALTVRPKKIN", "aa_hexdigest": "737f0a18fe7873f149c09b795d6154a2", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnc" ], "truncated": "both", "pseudogene": { "start": 659445, "stop": 660293, "observations": { "mutation": [ 659504, 660200 ], "stop": [ 659504, 660200 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75233", "query_cov": 1.0, "subject_cov": 0.7304964539007093, "identity": 0.995, "score": 407.0, "evalue": 1.52e-142, "gene_start": 1, "gene_end": 206, "reference_start": 58, "reference_end": 263, "reference_sequence": "MKNKKDKTQKPKVIDEKFVAFFKSLNIEPQNWQFYEDAFVHSSYVNENEDARASYDRLEFLGDALIDFIVAKKLFELYPNYNEGMLTRTKIEIVKGENLNRIGKELNFGNFIKLGKGMPYTETLFGDVLEALVAAIYEDLGIEKANQFVEEHIFKKTYSEILKYNFFSLFQEQKLPEPRVRVSLTSNNLVLSIIELNGDIIWSQAVPNSKHYDDKSVLEHNAMSAFTQFLKSGKGINFFSDIKNKLDSQKPMRALTVRPKKINWKARKPKLKALKNKVKADS" }, "paralog": false, "identity": 0.9929078014184397, "score": 552.0, "evalue": 3.94e-202, "description": "Internal stop codon at 659504, 660200. Nonsense mutation around 659504, 660200.", "psc": { "uniref90_id": "UniRef90_P75233", "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_P47607", "cog_id": "COG0571", "cog_category": "K", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75233", "EC:3.1.26.3", "UniRef:UniRef50_P47607", "COG:COG0571", "COG:K", "GO:0003723", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47607" ], "product": "Ribonuclease 3" } }, "id": "CNOCLNGIAL_1170", "locus": "GCA000143945_01169" }, { "type": "cds", "sequence": "contig_1", "start": 660198, "stop": 660293, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAGAAAGATAAAACTCAAAAACCTAAGGTCATTGATGAAAAGTTTGTCGCTTTTTTCAAGAGTTTAAACATTGAACCGCAAAACTGA", "aa": "MKNKKDKTQKPKVIDEKFVAFFKSLNIEPQN", "aa_hexdigest": "611bbaf44cf0d061be9744ba31977735", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3694.304400000001, "isoelectric_point": 9.778516197204588 }, "id": "CNOCLNGIAL_1171", "locus": "GCA000143945_01170" }, { "type": "cds", "sequence": "contig_1", "start": 660283, "stop": 661269, "strand": "-", "frame": 3, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811", "KEGG:K03621", "RefSeq:WP_014325596.1", "SO:0001217", "UniParc:UPI0001DD642C", "UniRef:UniRef100_A0AAN4XGJ0", "UniRef:UniRef50_Q49427", "UniRef:UniRef90_P75232" ], "nt": "ATGGCTTTTAGACTAGCAGTTGATTGTTTAGGTTTTGAAAACCACCCGCGTGAAGCAATTGACGCAGTATTAGAATACTGGAGCTATCATCAAGAGTTGGAATTTATCTTAGTTGGTGATGAAAAGACCTTTGACGGACTTGATTATCTACCTAAAAACATTACCAAGCAATTAGCCAGCTCTTGCATAGATATGACCGACACACCACTAACCGCACGGCGCAAGGTTAACAACTCAATGCAGAAAGCGATTAACCTTGTGCGTGATGGTGCTGCTGATGTTGTGATTTCCGCTGGCTCCTCGGCCGTTTATGCCTCTTTAACATACGATGGCTTTGGCAAAATCCATAAAGATGTTAAAAGTGCTTTTATGTCTTATGTACCAACTGCCAATAACGACTGGTTTTACTTTTTAGATGTTGGCGCTAACAAGAACTTTACGGGTAAAGAGCTCTATTTTTTGGGCCTAATGGCGGATATTTTTGTCAAAAAGACCACCAACAAAATTAGTCCACGTATAGCATTGCTAAACATTGGTACCGAAATCAATAAAGGTTTTGATTACCACCAGGAAGGCTACCAATTGCTAAACGAAGATAAGCACCTTAACTTCACTGGTTTCATTGAACCACGCTTCTTACTTGACGGGGTGTGTGATATCTTAGTGGCTGATGGTTACAGCGGTAATCTTGTGTTGAAATCAATGGAAGGCACCTTTAAAACAATTGCGCGTCTTCTCAAGCAAGGTTATAAGCGTAACCCGTTGGCTGGGCTTTTTAGTTTGGGAATTTTGAAAAGAATTGCTAAGCGTTTTGATTACAAAAATAACGCAGGCGCCGTTGTTATCGGTTTAAATAAACTTGCTTTAAAAACCCATGGATCAGCTGATAAACAACAGTTTTTAAGCACCATTAGATTAGCACACACTAGCTTAAAGTCTGATCTAATTAACGCCATTAAAAGTTCCTTAGATAACTATGAAAAATAA", "aa": "MAFRLAVDCLGFENHPREAIDAVLEYWSYHQELEFILVGDEKTFDGLDYLPKNITKQLASSCIDMTDTPLTARRKVNNSMQKAINLVRDGAADVVISAGSSAVYASLTYDGFGKIHKDVKSAFMSYVPTANNDWFYFLDVGANKNFTGKELYFLGLMADIFVKKTTNKISPRIALLNIGTEINKGFDYHQEGYQLLNEDKHLNFTGFIEPRFLLDGVCDILVADGYSGNLVLKSMEGTFKTIARLLKQGYKRNPLAGLFSLGILKRIAKRFDYKNNAGAVVIGLNKLALKTHGSADKQQFLSTIRLAHTSLKSDLINAIKSSLDNYEK", "aa_hexdigest": "0a52ba0fd5334dfc6f9b4806153468a7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD642C", "ncbi_nrp_id": "WP_014325596.1", "uniref100_id": "UniRef100_A0AAN4XGJ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD642C", "RefSeq:WP_014325596.1", "UniRef:UniRef100_A0AAN4XGJ0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAN4XGJ0", "uniref90_id": "UniRef90_P75232" }, "psc": { "uniref90_id": "UniRef90_P75232", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q49427", "cog_id": "COG0416", "cog_category": "I", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q49427", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49427" ], "product": "Phosphate acyltransferase" }, "genes": [ "plsX" ], "id": "CNOCLNGIAL_1172", "locus": "GCA000143945_01171" }, { "type": "cds", "sequence": "contig_1", "start": 661269, "stop": 662408, "strand": "-", "frame": 1, "gene": "fakA", "product": "putative MG369-like protein", "db_xrefs": [ "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071", "SO:0001217", "UniRef:UniRef50_P75231", "UniRef:UniRef90_P75231" ], "nt": "ATGCTCCCAGTGTTAAAGGCCTCTGGTGTAGTAGATTCAGGTGCTTATGGGTTCGCTTGCTTTTTAGAGGGGATGTTGTCCTTTTATGGTGAAAAGGCTACTTTAAACGATGGTAAGCTCACCAGTGCTGAATTGAGCCAAATGACCATTAGCGGTGAAAAACACGTTACGGAAGAAGAGTTTGGTTACTGCACTGAATATGTCTTGAAACTGGGCATGTCAGTAAGTCAAGAAGTTGAAAAGCAGAAGTTTAACCAAAAGAAGTTTGAAAGTAAGGTGAGCAAAATTGCCACTTCGGTAGTAGTTGCTAGTGATAAAGATAACGGCTTTGTTAAAGTACACGCCCATACCGAAAAACCTAACCTGTTATTAGAACTTGGGCTTAACTATGGTGAGTTTGAACTCGTCAAAATTGAAAACATGAATTTACAGGTAGCGAAGCAAAAACCCGCTCCTGTGAAGCGCAACATTAAACCAGCCATAGTAGTGACTGTACCAACTGAAGCTTTTGCTGATCGGATTCGCGAAGACTATGACATTCAAGCTATCTTGTGTACCGATGACACCGGTGCACCTTCGGTCTTTTCGTTACTAGAAGCAGTCAAGCTAACTCATTCCAGTAACATTATTTTCTTGTTACATGACAAGAACTACTTTCTCTCTGCCAACGAAGCGTTAAAACAATTAAAGCACCAAAAGATTAGTGCTGATTGTGTGATGACTACCAACCCGATTGAATCACTAGCGGCGCTTACTGTTTTTAACAGTGACCTTAACATTCACACCAATGTCAAAACAATGCGCCGTTTTGTCAAAGGCTTTGCATCTGCAACCATTACTCAAGCTTCGAAAAAGTACAAGGAAAATCGGATTGAAGTTAATAAGGGTGACTTTATTGCTGTAGCTAATAACAGCATTTGTGTTAGTGAAAAAGAGCTTGTTCAATGCGTGTTCAACACCATTGACCACCTTTTGAAAAAGGTAAAAAAGCCGGAGTTTCTTTTAGCTTACTATGGTAAAGATATCACCGCTGAGGAAGCGGAAGCAATGAAGGAAAAAATAGAAAAGAAATACAAGCTTTTCTGTGAGTTTTCCCCAGGGGAACAAAAAGTTTTTTCTTATATACTAGGCATACAATAA", "aa": "MLPVLKASGVVDSGAYGFACFLEGMLSFYGEKATLNDGKLTSAELSQMTISGEKHVTEEEFGYCTEYVLKLGMSVSQEVEKQKFNQKKFESKVSKIATSVVVASDKDNGFVKVHAHTEKPNLLLELGLNYGEFELVKIENMNLQVAKQKPAPVKRNIKPAIVVTVPTEAFADRIREDYDIQAILCTDDTGAPSVFSLLEAVKLTHSSNIIFLLHDKNYFLSANEALKQLKHQKISADCVMTTNPIESLAALTVFNSDLNIHTNVKTMRRFVKGFASATITQASKKYKENRIEVNKGDFIAVANNSICVSEKELVQCVFNTIDHLLKKVKKPEFLLAYYGKDITAEEAEAMKEKIEKKYKLFCEFSPGEQKVFSYILGIQ", "aa_hexdigest": "1305cb2248b8843c5d3ec1e68f7fc9ab", "start_type": "ATG", "rbs_motif": null, "genes": [ "fakA" ], "truncated": "3-prime", "pseudogene": { "start": 661269, "stop": 662707, "observations": { "deletion": [ 662683 ], "stop": [ 662434 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75231", "query_cov": 1.0, "subject_cov": 0.6792114695340502, "identity": 1.0, "score": 732.0, "evalue": 1.09e-263, "gene_start": 1, "gene_end": 379, "reference_start": 180, "reference_end": 558, "reference_sequence": "MSTVNLSNFIDMLRLGCKNIANNFEYINQLNVFPVPDGDTGTNMKVTLSEAFKKLESEISHIKSFSDLGKSFTRDLLLFSRGNSGVIFSQIMKGFFSDMISTKTATETELGIEDFATAFIKAEEVAYKNVSKPVEGTMLTVIRLISTDFKNQKNRAKTVQKLFEQVIKTAWQTVKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSFYGEKATLNDGKLTSAELSQMTISGEKHVTEEEFGYCTEYVLKLGMSVSQEVEKQKFNQKKFESKVSKIATSVVVASDKDNGFVKVHAHTEKPNLLLELGLNYGEFELVKIENMNLQVAKQKPAPVKRNIKPAIVVTVPTEAFADRIREDYDIQAILCTDDTGAPSVFSLLEAVKLTHSSNIIFLLHDKNYFLSANEALKQLKHQKISADCVMTTNPIESLAALTVFNSDLNIHTNVKTMRRFVKGFASATITQASKKYKENRIEVNKGDFIAVANNSICVSEKELVQCVFNTIDHLLKKVKKPEFLLAYYGKDITAEEAEAMKEKIEKKYKLFCEFSPGEQKVFSYILGIQ" }, "paralog": false, "identity": 0.9979123173277662, "score": 924.0, "evalue": 0.0, "description": "Internal stop codon at 662434. Frameshift due to deletion around 662683.", "psc": { "uniref90_id": "UniRef90_P75231", "gene": "FakA", "product": "Uncharacterized protein MG369 homolog", "uniref50_id": "UniRef50_P75231", "cog_id": "COG1461", "cog_category": "I", "go_ids": [ "GO:0004371", "GO:0006071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75231", "UniRef:UniRef50_P75231", "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071" ] }, "pscc": { "uniref50_id": "UniRef50_P75231", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75231" ], "product": "Uncharacterized protein MG369 homolog" } }, "id": "CNOCLNGIAL_1173", "locus": "GCA000143945_01172" }, { "type": "cds", "sequence": "contig_1", "start": 662433, "stop": 662672, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGGCTTTTTTAGTGACATGATTAGCACTAAAACAGCCACCGAAACCGAACTTGGCATAGAGGATTTTGCTACAGCTTTTATAAAGGCCGAAGAGGTGGCATACAAGAATGTCTCCAAGCCCGTAGAGGGTACGATGCTCACGGTTATTCGCTTAATTAGCACAGACTTTAAAAACCAAAAGAACCGTGCCAAAACAGTGCAAAAACTGTTTGAACAAGTAATTAAAACAGCCTGA", "aa": "MKGFFSDMISTKTATETELGIEDFATAFIKAEEVAYKNVSKPVEGTMLTVIRLISTDFKNQKNRAKTVQKLFEQVIKTA", "aa_hexdigest": "99ef2eb6a15f64c61dc36bcc1145a201", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8879.193899999997, "isoelectric_point": 9.003799629211429 }, "id": "CNOCLNGIAL_1174", "locus": "GCA000143945_01173" }, { "type": "cds", "sequence": "contig_1", "start": 662669, "stop": 662944, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTACAGTAAATTTAAGCAACTTTATTGACATGTTGCGTCTTGGTTGTAAGAATATAGCTAATAATTTCGAATACATTAATCAACTCAATGTTTTCCCGGTGCCAGATGGTGATACGGGGACAAACATGAAGGTCACGTTGAGTGAAGCGTTCAAAAAACTAGAGAGTGAAATTAGCCACATTAAGAGCTTTAGTGATTTGGGCAAAAGCTTTACCCGTGACCTTTTACTTTTCTCACGCGGTAACTCTGGTGTTATTTTTCCCAAATTATGA", "aa": "MSTVNLSNFIDMLRLGCKNIANNFEYINQLNVFPVPDGDTGTNMKVTLSEAFKKLESEISHIKSFSDLGKSFTRDLLLFSRGNSGVIFPKL", "aa_hexdigest": "a097783ad22880d0e2b793c3bc52a697", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10176.59379999999, "isoelectric_point": 7.880373573303222 }, "id": "CNOCLNGIAL_1175", "locus": "GCA000143945_01174" }, { "type": "cds", "sequence": "contig_1", "start": 663032, "stop": 663367, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAACAGTTAAGGCCAAACCATTTCCTAACGCTAAACCGATTACCACCATTTTTGAAAACAGTAAGTACTTAACTAAGTTAGATCTTTCGTTGCTTACTATACGCTTAGTAAGTGGTAAAACCCACCAAATTCGTGCTTGCTTAAACCTCTTGAATACCCAGTTAGTTGGTGATAGAAAATACCAATTACTGCAATTTAAAAACCGTAACCGCGATTTTAAACACCAAGCCTTACATGCCACAAATCTAAGTTTTGCCCAATTAGATAAGCAGAAATTTCCCTTACTGGCTAATTACTCGCAACGGCAGTTTAAAAGCCAACTAGTACCGTGA", "aa": "MVTVKAKPFPNAKPITTIFENSKYLTKLDLSLLTIRLVSGKTHQIRACLNLLNTQLVGDRKYQLLQFKNRNRDFKHQALHATNLSFAQLDKQKFPLLANYSQRQFKSQLVP", "aa_hexdigest": "c96dc94f449ef1bc5722ce1f013a8783", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12855.907599999984, "isoelectric_point": 10.4221040725708 }, "id": "CNOCLNGIAL_1176", "locus": "GCA000143945_01175" }, { "type": "cds", "sequence": "contig_1", "start": 663389, "stop": 663979, "strand": "-", "frame": 2, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG370-like protein", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0120159", "SO:0001217", "UniRef:UniRef50_P47610", "UniRef:UniRef90_P75230" ], "nt": "ATGTCAACAGCTAAGTTTGTAGTGCCTAAAGCTGTTGAAAACGTTAGGATTGAAAAGTTTTGTTTAAAGTTGTTGCCCAACATTAAGCTAAGTCAGTTCTTTAAATTGTTGCGCTTAGGTAAGGTCTTGCTCAATAACACGAAGGCTAAATTAGGTCAGCGTGTTAGCAGCGGTGATACAATCGTTTTTCAGTTTGCAATTGAACCTTACTTACACAACCACAGCGAAGTAGTGGATTTAACCCAAGTCCAAGATGATTTGCAGGTAATCTTTGAGGATGAACAGCTCATTGTGGTGGACAAACCAGCGGGTGTAGTGTGTCAACCCGATGCTAAACACGAACTGTTTAATTTAGCTAACAGCTTACTGAAACACTGTGGTTATAATCAATACTTTAAAAACAGTCTTAGCTTCATACCACGGTTTGCGCACCGAATTGATCGTAATACCTGTGGTTTGGTAATAGGTGCTAAAACCAACCAGGCATTACAGGAGTTAGAGGCGGTTTTTCGCCATAACTTGTTAATGAAACAATACCAAGGATTAGTGTTTGGCCCATTTAATTTTAAGGGCACACAAAAAGCTTATTGA", "aa": "MSTAKFVVPKAVENVRIEKFCLKLLPNIKLSQFFKLLRLGKVLLNNTKAKLGQRVSSGDTIVFQFAIEPYLHNHSEVVDLTQVQDDLQVIFEDEQLIVVDKPAGVVCQPDAKHELFNLANSLLKHCGYNQYFKNSLSFIPRFAHRIDRNTCGLVIGAKTNQALQELEAVFRHNLLMKQYQGLVFGPFNFKGTQKAY", "aa_hexdigest": "6e8b5124e74d0f1360d9faa9fdf7b300", "start_type": "ATG", "rbs_motif": null, "genes": [ "rluA" ], "truncated": "5-prime", "pseudogene": { "start": 663086, "stop": 663979, "observations": { "mutation": [ 663391 ], "stop": [ 663391 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75230", "query_cov": 1.0, "subject_cov": 0.6012269938650306, "identity": 1.0, "score": 390.0, "evalue": 1.67e-135, "gene_start": 1, "gene_end": 196, "reference_start": 1, "reference_end": 196, "reference_sequence": "MSTAKFVVPKAVENVRIEKFCLKLLPNIKLSQFFKLLRLGKVLLNNTKAKLGQRVSSGDTIVFQFAIEPYLHNHSEVVDLTQVQDDLQVIFEDEQLIVVDKPAGVVCQPDAKHELFNLANSLLKHCGYNQYFKNSLSFIPRFAHRIDRNTCGLVIGAKTNQALQELEAVFRHNLLMKQYQGLVFGPFNFKGTQKAYWAKDAYQALVTVKAKPFPNAKPITTIFENSKYLTKLDLSLLTIRLVSGKTHQIRACLNLLNTQLVGDRKYQLLQFKNRNRDFKHQALHATNLSFAQLDKQKFPLLANYSQRQFKSQLVPWFASLIKGVSI" }, "paralog": false, "identity": 0.9966329966329966, "score": 588.0, "evalue": 1.34e-215, "description": "Internal stop codon at 663391. Nonsense mutation around 663391.", "psc": { "uniref90_id": "UniRef90_P75230", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG370 homolog", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47610", "cog_id": "COG0564", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0120159", "GO:0000455" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75230", "EC:5.4.99.-", "UniRef:UniRef50_P47610", "COG:COG0564", "COG:J", "GO:0003723", "GO:0120159", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47610", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47610" ], "product": "Uncharacterized RNA pseudouridine synthase MG370" } }, "id": "CNOCLNGIAL_1177", "locus": "GCA000143945_01176" }, { "type": "cds", "sequence": "contig_1", "start": 663966, "stop": 664088, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTGGTGGTGGGCACAAGTATGCTGCTGCCTTTGTGTTGGATGACAAGCGTCAGTTTATGAAGTTAGTGGAAATTATGGATGACTTTTTAGCCAAACAAAAGCATGTCAACAGCTAA", "aa": "MFGGGGHKYAAAFVLDDKRQFMKLVEIMDDFLAKQKHVNS", "aa_hexdigest": "6ad50d8c0e8924b3ec1958914c3de93b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4544.239600000001, "isoelectric_point": 8.140052223205569 }, "id": "CNOCLNGIAL_1178", "locus": "GCA000143945_01177" }, { "type": "cds", "sequence": "contig_1", "start": 664137, "stop": 664943, "strand": "-", "frame": 1, "gene": "nrnA", "product": "putative MG371-like protein", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.13.3", "EC:3.1.3.7", "GO:0003676", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_P75229", "UniRef:UniRef90_P75229" ], "nt": "ATGATTAACATCGATCCCCATTTTATTCATAACCTAACTAATAAACTCAAGACATTCGATAACTTTTCGTTATATGTGCATGTAAACCCGGACTTTGATGCCTTTGGCGCTGCTTTTGCCTTTAAGGCCTTTTTAGCGGTTTACTTCCCCCATAAAAAGGCTTACGTGATGGGATCACACAACATTAAGGCTGACGGGAAAGATTTGTTCCCCTTTGAAGCGGCACCTATTGATGATGCGTTTGTAAAAAACTCATTGGCAATTATCTTTGACACCTCCAACCAAGAACGAGTATTAACCCAAAAACATAAATTAGCTAAAGAAACCGTGCGGATTGATCACCATCCTAAAACCGAGAGTTTTGCTGATCTGGAATGGATTGACCCGGCTTTTTCAGCAGCAGCAGAAATGGTCGGTTACTTAATCTTGCAAATGGGTTATGAACTTAATGCTGAGATGGCTGCTTACATTTATGCGGGTATTATTACCGATACACAGCGCTTTTCCAGTTCCGCTACCACCCCACAAACCTTTGCTTTAACTGCTAAGTTACTAGAAACTGGATTTAACCGCAATAAGGTTCATGATGCAGTTTATCTAAAACCACTTTTAGAACACAAGTACTTTAGCTATGTCTTAAACAAAGCCAAGATCACCCCGAATGGTTTGGCTTACGCACTTCTTAAAAAAGGGACTTATAAGCAGTTTGGGGTGGTCTCACCACTACCGATGGTGCACGCTTTAAACAACATCAAAGGAGTCAAAATTTGGACGACTTGTTACTTTAACGAAGACATTAAGAAGTGA", "aa": "MINIDPHFIHNLTNKLKTFDNFSLYVHVNPDFDAFGAAFAFKAFLAVYFPHKKAYVMGSHNIKADGKDLFPFEAAPIDDAFVKNSLAIIFDTSNQERVLTQKHKLAKETVRIDHHPKTESFADLEWIDPAFSAAAEMVGYLILQMGYELNAEMAAYIYAGIITDTQRFSSSATTPQTFALTAKLLETGFNRNKVHDAVYLKPLLEHKYFSYVLNKAKITPNGLAYALLKKGTYKQFGVVSPLPMVHALNNIKGVKIWTTCYFNEDIKK", "aa_hexdigest": "7ecaa9433da3a58a934864d1378b3540", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75229", "query_cov": 1.0, "subject_cov": 0.8246153846153846, "identity": 1.0, "score": 538.0, "evalue": 9.88e-193, "valid": true, "gene": "NrnA", "product": "Uncharacterized protein MG371 homolog", "ec_ids": [ "3.1.13.3", "3.1.3.7" ], "uniref50_id": "UniRef50_P75229", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "go_ids": [ "GO:0003676" ] }, "pscc": { "uniref50_id": "UniRef50_P75229", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75229" ], "product": "Uncharacterized protein MG371 homolog" }, "genes": [ "nrnA" ], "id": "CNOCLNGIAL_1179", "locus": "GCA000143945_01178" }, { "type": "cds", "sequence": "contig_1", "start": 664936, "stop": 665073, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACCTGTACTGTGTTTGCTCCCAAAAAACCAACTACTAAGGCTAAGTTAGCGATCGTTGAAAAGCTTGAGAGTGAACTTTTGTTTGTCCGTGAACTAATTGAAGCTGGTGTCAAGAAGCTGCAAAATGATTAA", "aa": "MDTCTVFAPKKPTTKAKLAIVEKLESELLFVRELIEAGVKKLQND", "aa_hexdigest": "b23bbd64bd2dfe4cacb12c9dbca1bfea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5046.941199999999, "isoelectric_point": 7.871863746643066 }, "id": "CNOCLNGIAL_1180", "locus": "GCA000143945_01179" }, { "type": "cds", "sequence": "contig_1", "start": 665116, "stop": 666099, "strand": "-", "frame": 3, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "EC:2.8.1.4", "GO:0000049", "GO:0002937", "GO:0004810", "GO:0005524", "GO:0005829", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0140741", "KEGG:K03151", "SO:0001217", "UniRef:UniRef50_P47612", "UniRef:UniRef90_P75228" ], "nt": "ATGGGATTGCATAATGAACCAAATACTATTCTAATCTGCCGTTATGGCGAACTTGTTTTAAAGGGCAAGAACCGCTTGCAGTTTGTCAAACAACTCAAAAAGAACGTTAAACAAGCCTTTAAGAAACTAAGCATTACTAACCCAGTTGACTACCAGTTTGACATGTTGGTAGTGGGTGAAGTAATTTCCACCCAAAGATCGTTATTAAAAAATCTCTTTACCAGATTACCGGGTTTGAGTGTTTGCTTGTTTGCCTTACAAATCCCTCATGATGAAGCGCAATTGTTAGCACTGCTACAGCAAGTGGTACAATCCCACCCATCGTTCAAAATTGAGGTAAGACGGCGTGATAAATTATTTGCTTGCAATTCCAGTGCCTTCAAAAAGTACCTAGCTTTACAGTTATGGGAAAAATACCAATTGAAGGGTAAGTTAGTTGACCCTGCCATTACGGTGCATGTCGAAGTAACTAAGGAACATTTCCTAATAATTAGTGAAAGCTTTAATGGTATTGGTGGTCTACCGGTCTTTACTAGTGGTACTGCTTTGGCGTTACTGTCTGGTGGCATAGACAGTCCAGTAGCCGCTTCCCTAGTTTTACAACGAGGTTTTAATGTTGACTTTATTACTTTTATTAATGAGCCCGGACATAACGCTGCCACAATAGGCAAAATTCAACGGTTAGCTAACTTAGTTTCCCTTAACCAAACGCTTTGTACTGGGAGACTGTTTGTCTTTGACTTTACCGATTTACAGAAAGAGTTGAGTCATATTAGTCTGGAAGGATACCGGATTGTTTTAATGCGCCGTTGCTTTTACAAGATTGCTAGTTTGTTTAAATATGATTGTTTAATCACCGGGGAAGCATTGGGACAAGTAGCTTCACAAACAATTGATAATTTGAAGGTAATCCAAGCTGTTGTGCCCAATACCTTTGTGATTCGCCCGTTAATAGGCTTAAGTAAAGATAAAATAATTGAATGA", "aa": "MGLHNEPNTILICRYGELVLKGKNRLQFVKQLKKNVKQAFKKLSITNPVDYQFDMLVVGEVISTQRSLLKNLFTRLPGLSVCLFALQIPHDEAQLLALLQQVVQSHPSFKIEVRRRDKLFACNSSAFKKYLALQLWEKYQLKGKLVDPAITVHVEVTKEHFLIISESFNGIGGLPVFTSGTALALLSGGIDSPVAASLVLQRGFNVDFITFINEPGHNAATIGKIQRLANLVSLNQTLCTGRLFVFDFTDLQKELSHISLEGYRIVLMRRCFYKIASLFKYDCLITGEALGQVASQTIDNLKVIQAVVPNTFVIRPLIGLSKDKIIE", "aa_hexdigest": "88c17433a7a858e83239292eb5fef1b1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75228", "query_cov": 1.0, "subject_cov": 0.8449612403100775, "identity": 1.0, "score": 635.0, "evalue": 6e-229, "valid": true, "gene": "thiI", "product": "Probable tRNA sulfurtransferase", "ec_ids": [ "2.8.1.4" ], "uniref50_id": "UniRef50_P47612", "cog_id": "COG0301", "cog_category": "HJ", "kegg_orthology_id": "K03151", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0004810", "GO:0000049", "GO:0140741", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0002937" ] }, "pscc": { "uniref50_id": "UniRef50_P47612", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47612" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [ "thiI" ], "id": "CNOCLNGIAL_1181", "locus": "GCA000143945_01180" }, { "type": "cds", "sequence": "contig_1", "start": 666110, "stop": 666757, "strand": "-", "frame": 2, "gene": null, "product": "MPN551 family DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227", "UniRef:UniRef90_P75227" ], "nt": "ATGGTCAAGATTTATGAGGATGAATTTGATGAAACACTCTCACGCGCGGGGCAAGTTATTGAACCCAAAATCCGTGATTATGTGATAGCTAAAACTGGTTTTAATTTCCATTCGTATGATCCCAAGGAGGTTAAGTGGGATCTCTTTCCTGAAAATCCAGTTTTTGGAGGAATTCCTGATGGGGAACCGGTCGATGTTTATGGAAAATTGGCTTACGATACCAATGCGCCGATGTTAGAAATTAAAACCACTTCCTGTGATTCTTTGGTCTATAAAAAGATTAACGGTAACCTCAAAATTGTGTTTGATGAAAACGGCATGCCGATTGTCAAAAAGATTAACGGAAAGAAAGATAGTTGGTTTGACAGTAACGGCAAAATCGTGATTAGCCCGGCTTACTATTGCCAAATTGGGTTGTATTTGTACTTGCGCAATATTACCAAAGGAATGTTTGCGATTGCTTTTTTAGAACCACAAGACTATGTGCATCCCGAATGGTTTGAAGCAAAGCAACGCGACATCCGTTTGGTACCAGTACAAATTGATCGCAAGGCCTTTGAAGTTTTAACTAATAAAGCACAGCTGTGGTACAACAGTTTTATTCGTACTGGTAAAAGTCCACAATTAACCTCACAAGACTGGGAGTGA", "aa": "MVKIYEDEFDETLSRAGQVIEPKIRDYVIAKTGFNFHSYDPKEVKWDLFPENPVFGGIPDGEPVDVYGKLAYDTNAPMLEIKTTSCDSLVYKKINGNLKIVFDENGMPIVKKINGKKDSWFDSNGKIVISPAYYCQIGLYLYLRNITKGMFAIAFLEPQDYVHPEWFEAKQRDIRLVPVQIDRKAFEVLTNKAQLWYNSFIRTGKSPQLTSQDWE", "aa_hexdigest": "c4594b244de3c8e7953141338c4ad21f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 666086, "stop": 666931, "observations": { "mutation": [ 666112, 666766 ], "stop": [ 666112, 666766 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75227", "query_cov": 1.0, "subject_cov": 0.7651245551601423, "identity": 1.0, "score": 410.0, "evalue": 8.99e-144, "gene_start": 1, "gene_end": 215, "reference_start": 59, "reference_end": 273, "reference_sequence": "MGFTKQYKIDFDIIDNQIVLSNNYFQKHKGSFRKITGTRFGKVIGISEYETSLKTWANMVKIYEDEFDETLSRAGQVIEPKIRDYVIAKTGFNFHSYDPKEVKWDLFPENPVFGGIPDGEPVDVYGKLAYDTNAPMLEIKTTSCDSLVYKKINGNLKIVFDENGMPIVKKINGKKDSWFDSNGKIVISPAYYCQIGLYLYLRNITKGMFAIAFLEPQDYVHPEWFEAKQRDIRLVPVQIDRKAFEVLTNKAQLWYNSFIRTGKSPQLTSQDWEWLRENGIA" }, "paralog": false, "identity": 0.9928825622775801, "score": 534.0, "evalue": 1.09e-194, "description": "Internal stop codon at 666112, 666766. Nonsense mutation around 666112, 666766.", "psc": { "uniref90_id": "UniRef90_P75227", "product": "MPN551 family DNA-binding protein", "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75227", "UniRef:UniRef50_P75227" ] }, "pscc": { "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227" ], "product": "Uncharacterized protein MG373 homolog" } }, "id": "CNOCLNGIAL_1182", "locus": "GCA000143945_01181" }, { "type": "cds", "sequence": "contig_1", "start": 666764, "stop": 666931, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTTACCAAACAGTACAAAATCGATTTTGATATTATTGACAACCAAATAGTTTTGTCAAACAACTATTTTCAAAAACATAAAGGTAGTTTTCGCAAGATTACCGGTACCCGATTTGGTAAAGTAATAGGTATTTCTGAGTATGAAACTTCACTTAAAACTTGA", "aa": "MGFTKQYKIDFDIIDNQIVLSNNYFQKHKGSFRKITGTRFGKVIGISEYETSLKT", "aa_hexdigest": "cc6b4530b501482e28ce0c7bb25e98ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6407.267899999999, "isoelectric_point": 9.630819129943848 }, "id": "CNOCLNGIAL_1183", "locus": "GCA000143945_01182" }, { "type": "cds", "sequence": "contig_1", "start": 666938, "stop": 667600, "strand": "-", "frame": 2, "gene": null, "product": "MPN552 family protein", "db_xrefs": [ "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75226", "UniRef:UniRef90_P75226" ], "nt": "GTGTATCTGTTAGATTTACTTTCACTCAACAACGTTGATGTAATTGCGGAATCAGGTGCCATTACCTGGTTTTGCAAAACCAATCAGTATGACTATCAAGCCTTTTTAGACCTCGCTAGTATTAATGTCATTATCCACCACGCTGTGATTACCAATTCCGGGATCTTTACCATGGGTAAAAGCCGCGTTGATGATACCGCTAATTTATCAGCTAACTATTTCATTTCACTACAAAAGTACAAGGACTTTAAGTCGCTGTGGTTAACGGACTTTGAACAAACCTTAAAGTACGAAAACTTTTTAAAACAACTCGGTAAATTGGAACTCAGTTCTATCTACGTCTTTAGTCCCCAATACCACATGGACTTAGCTTTTATTGACCAAATTGCTAGTGGTCAACCACGCTTTACCCACAGCAATTTCTATCCCAATAACCTGTTGTTTACAAGTAATAAAGTAACTAAATTTAATGCACTGGAAAAATACACTAAGACCCAAGGCTTAATGCTTAAAGATGTGCACTACATTAACTTAGATGAAACTTTGGTGCAAAACAGTGAGCAGTTAGCTAGTGCTGTTTTCATCAAGAAACAGAACAATGAAGGTTTAACAGTGCAGGACATTCCTGATGTGTTACAAAAGCTTTGTGCACAACTCTTGTAA", "aa": "MYLLDLLSLNNVDVIAESGAITWFCKTNQYDYQAFLDLASINVIIHHAVITNSGIFTMGKSRVDDTANLSANYFISLQKYKDFKSLWLTDFEQTLKYENFLKQLGKLELSSIYVFSPQYHMDLAFIDQIASGQPRFTHSNFYPNNLLFTSNKVTKFNALEKYTKTQGLMLKDVHYINLDETLVQNSEQLASAVFIKKQNNEGLTVQDIPDVLQKLCAQLL", "aa_hexdigest": "ff2b4e6e901bfbcab640c7cd4c669444", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75226", "query_cov": 1.0, "subject_cov": 0.8178438661710037, "identity": 0.995, "score": 438.0, "evalue": 7.68e-155, "valid": true, "product": "MPN552 family protein", "uniref50_id": "UniRef50_P75226", "go_ids": [ "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_P75226", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75226" ], "product": "Uncharacterized protein MG374 homolog" }, "genes": [], "id": "CNOCLNGIAL_1184", "locus": "GCA000143945_01183" }, { "type": "cds", "sequence": "contig_1", "start": 667776, "stop": 668036, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGGCACCAGTACAAGTAGTGGTGATTCCGGTTAACATCGATAAACACGAAAAGGCTGCCAAAAAACTGTACCAAAAATTGTTAAAGGAAAACATTAGGGTCAGCCTAGATGAGAGCGAAGATCGTTTGGGCAAAAAAGTTCGCACAGCCATTGTTAACAAGATTCCACTGCAAATTATTGTCGGCGATAAGGAGATGCAGGATCTTACTCGAATAACTTGTCGTGGCTTTAAAGGTGAGAAGGTACAGAAACTGTATTGA", "aa": "MAPVQVVVIPVNIDKHEKAAKKLYQKLLKENIRVSLDESEDRLGKKVRTAIVNKIPLQIIVGDKEMQDLTRITCRGFKGEKVQKLY", "aa_hexdigest": "f0d2dd0b06db86d9cf87c7e0ae22a61e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 80, "aa_cov": 0.9069767441860465, "hmm_cov": 0.9875, "evalue": 1.8133731078438494e-21, "score": 71.61233520507812, "start": 5, "stop": 82 } ], "seq_stats": { "molecular_weight": 9857.613199999996, "isoelectric_point": 9.716755485534666 }, "id": "CNOCLNGIAL_1185", "locus": "GCA000143945_01184" }, { "type": "cds", "sequence": "contig_1", "start": 668037, "stop": 668315, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "GTGTTTAAGGAGTTAAATGTGCCCTATAAAATTGATGTGGGTGCAGCTGCTTTTTATGGACCTAAAATTGACTTTCAGTTTAAAACGGCACTCAATAAGATGATTACCATTGCCACTATTCAACTGGATTTTTTACTGCCAGAACGCTTTGAATTGCGTTACTTTGATGAGCAGAGCAATGCTCAAACTCCCGTAATTATTCACGTTGGCATAGTCGGTACGTACGAGCGTTTTATTGCTGCTTTATTGGAAAAAACTCATGGCAATCTACCGTTGTGA", "aa": "MFKELNVPYKIDVGAAAFYGPKIDFQFKTALNKMITIATIQLDFLLPERFELRYFDEQSNAQTPVIIHVGIVGTYERFIAALLEKTHGNLPL", "aa_hexdigest": "fda05000102537b83a3dfef45eb140e3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 72, "aa_cov": 0.7608695652173914, "hmm_cov": 0.9722222222222222, "evalue": 3.723310419948842e-15, "score": 51.888248443603516, "start": 16, "stop": 85 } ], "seq_stats": { "molecular_weight": 10505.126599999989, "isoelectric_point": 6.054114341735839 }, "id": "CNOCLNGIAL_1186", "locus": "GCA000143945_01185" }, { "type": "cds", "sequence": "contig_1", "start": 668343, "stop": 668861, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGGAAAAGGGAGCAGTTTTAAAAAAGATTATTGGTGATTTTGTCCACTACCAACAGTTAATGTTTGGTTTTAAAACGGTATCTTCACCAGTATTAGCTAACTTAGAGCTGTACAAGATTAGTGGTCACTATGTGCACTATAGTGAGGACATGTTCCCCTCTGTCAAATTAGAAAACCAAATGATGATGCTCCGGCCAATGACCTGTCCGCACCACTGTTTGATTTTCAAGCACAAACGCCGCTCTTACAAAGAGTTACCACAACGCTTCTGTGAGGATTCCATCTTGCACCGCTTTGAAGCATCGGGTGGGCTTACTGGTTTGGAACGGGTAAGGTGCATGACACTGCTCGACAACCACATCTTTTGTCGTACTGACCAAATTAAAGCTGAGATTAAAAATGCCTTTAAGTTAATCCAAACGGTCAACAACAAGTTTGGTTTTAGCTTTGACCGAATTGACTTGGCCTTACACGATCCTAATAACAAGGCCAAATTCATTGACAACGATCAGTTATGA", "aa": "MEKGAVLKKIIGDFVHYQQLMFGFKTVSSPVLANLELYKISGHYVHYSEDMFPSVKLENQMMMLRPMTCPHHCLIFKHKRRSYKELPQRFCEDSILHRFEASGGLTGLERVRCMTLLDNHIFCRTDQIKAEIKNAFKLIQTVNNKFGFSFDRIDLALHDPNNKAKFIDNDQL", "aa_hexdigest": "2b75d7c9c35898e5159a9b5f73e2e4ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 104, "aa_cov": 0.5930232558139535, "hmm_cov": 0.9807692307692307, "evalue": 2.64442446285484e-20, "score": 68.66114807128906, "start": 58, "stop": 159 } ], "seq_stats": { "molecular_weight": 20113.2449, "isoelectric_point": 8.953191947937015 }, "id": "CNOCLNGIAL_1187", "locus": "GCA000143945_01186" }, { "type": "cds", "sequence": "contig_1", "start": 668862, "stop": 669326, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAAGAGCTTTTCCATTAAAGAGTTTGAACAGCTCGAACAGGACTTAAATGCTTATTTTAATCAGTTAAATAATGTTGTTCAGACAAAAATTGATAAGACAAAATCACTCAAATTTTTTCAAGCTGATCCGTTTACAACCACGCTTATTGAAAGTATAGAAAGCAACAGCCTTGTAGTTACCACCGTAAATGAACAAACATTTTGGGTGCATGATTTAGTGTTGCCATTACCCAAACAGGGCTTTGTGAAACTGCTCAATGTTAGTGCCAACTATTTTTTAGGTGATCCCACAAAACCACAACTACAAAGGCTTGTGGGAATTTTTGCAGCCTCTAAAGAACAGCTTAAAAGTCTTATTGCGGCTAATGAACAAAAATACCAAAATGATCACCGTGCTATTGGTAAGCGTTTAGAGATCTTTACCTTTGATCCCTTAGTCGGTGCAGGCTTTCCGATTTGA", "aa": "MEKSFSIKEFEQLEQDLNAYFNQLNNVVQTKIDKTKSLKFFQADPFTTTLIESIESNSLVVTTVNEQTFWVHDLVLPLPKQGFVKLLNVSANYFLGDPTKPQLQRLVGIFAASKEQLKSLIAANEQKYQNDHRAIGKRLEIFTFDPLVGAGFPI", "aa_hexdigest": "5b7ca69845cf2cf926b94364f10abb75", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17524.850199999983, "isoelectric_point": 6.116296195983886 }, "id": "CNOCLNGIAL_1188", "locus": "GCA000143945_01187" }, { "type": "cds", "sequence": "contig_1", "start": 669506, "stop": 669661, "strand": "-", "frame": 2, "gene": null, "product": "putative MG376-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224", "UniRef:UniRef90_P75224" ], "nt": "ATGCGCTTTTTTGGCGAGCGTTTATTTACTGACTATTACGTCATTTATGCCAATGGTCAACTGGCTTACGAATTGGAGAAACACACCAAGAAGTACAAGACCATTAGTATTGAAGGCATTTTGCGCACTTATTTAGAGCGTAAGAGTGAAATTTGA", "aa": "MRFFGERLFTDYYVIYANGQLAYELEKHTKKYKTISIEGILRTYLERKSEI", "aa_hexdigest": "46c870f8326c63aabc3fc298666ec418", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 669434, "stop": 669748, "observations": { "mutation": [ 669508, 669703 ], "stop": [ 669508, 669703 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75224", "query_cov": 1.0, "subject_cov": 0.4636363636363636, "identity": 1.0, "score": 102.0, "evalue": 5.16e-27, "gene_start": 1, "gene_end": 51, "reference_start": 36, "reference_end": 86, "reference_sequence": "GGGGGGMLNRVFLEGEIESSCWSVKKTGFLVTIKQMRFFGERLFTDYYVIYANGQLAYELEKHTKKYKTISIEGILRTYLERKSEIWKTTIEIVKIFNPKNEIVIDYKEI" }, "paralog": false, "identity": 0.9807692307692307, "score": 200.0, "evalue": 4.23e-68, "description": "Internal stop codon at 669508, 669703. Nonsense mutation around 669508, 669703.", "psc": { "uniref90_id": "UniRef90_P75224", "product": "Uncharacterized protein MG376 homolog", "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75224", "UniRef:UniRef50_P75224" ] }, "pscc": { "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224" ], "product": "Uncharacterized protein MG376 homolog" } }, "id": "CNOCLNGIAL_1189", "locus": "GCA000143945_01188" }, { "type": "cds", "sequence": "contig_1", "start": 669750, "stop": 670331, "strand": "-", "frame": 1, "gene": null, "product": "MPN555 family protein chaperone", "db_xrefs": [ "GO:0006457", "GO:0015031", "RefSeq:WP_014325598.1", "SO:0001217", "UniParc:UPI0001DD642D", "UniRef:UniRef100_A0AAX0SSF1", "UniRef:UniRef50_P75223", "UniRef:UniRef90_P75223" ], "nt": "ATGGCTACAAATCTTAAATCAACCGCTAAACTAGTTAAACCAATTCAGTACGATAAGGTAATTGAAGTAGAACGCATCTTTGCTGACCCTGCTTTTATTGAACAACACCGCCAACGGATCTTAGCTTCCTTTAAGGACGCTAAGGAAAGTGCGCTTTATCATGAACTAACGCACATTGTCATTAAGGACAACCTCTTTTCCTGTGCCATGAATGCTATTGTGGGTTACTTTGAATTCAACATCGATGAAGCTGAATTGAAGAATGTCATGGAAGGTCTAAAGCGTGATGTGATTCAGGGCGCTGAGGACAACACCGTGCAAGCAATTGCTGAAAAAATTATTAAGAAGGCTTTAGTGTTTAACCACCTCCAAAAAGAATGGAAAGTGGAAATCACTGATGAAGTGGTGAAGAATGTAATTAGTCTTTATTACGAAAAAACCAATCAAAGTGTGCGCGAATACTTGGATGATAAACAAAAGTTTGAGGGTGTGCGTACAGCCTTGCTAGAAGAGCGCATGGTGTTAGAAACCATCAACCACTTTAAATTTCACTTTAACTTAACTGGACAATTACCAAACTAA", "aa": "MATNLKSTAKLVKPIQYDKVIEVERIFADPAFIEQHRQRILASFKDAKESALYHELTHIVIKDNLFSCAMNAIVGYFEFNIDEAELKNVMEGLKRDVIQGAEDNTVQAIAEKIIKKALVFNHLQKEWKVEITDEVVKNVISLYYEKTNQSVREYLDDKQKFEGVRTALLEERMVLETINHFKFHFNLTGQLPN", "aa_hexdigest": "21a9954fee74d8d30aa974a9214b512b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD642D", "ncbi_nrp_id": "WP_014325598.1", "uniref100_id": "UniRef100_A0AAX0SSF1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD642D", "RefSeq:WP_014325598.1", "UniRef:UniRef100_A0AAX0SSF1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SSF1", "uniref90_id": "UniRef90_P75223" }, "psc": { "uniref90_id": "UniRef90_P75223", "product": "MPN555 family protein chaperone", "uniref50_id": "UniRef50_P75223", "go_ids": [ "GO:0006457", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75223" ], "product": "Uncharacterized protein MG377 homolog" }, "genes": [], "id": "CNOCLNGIAL_1190", "locus": "GCA000143945_01189" }, { "type": "cds", "sequence": "contig_1", "start": 670374, "stop": 671162, "strand": "-", "frame": 1, "gene": "argS", "product": "arginine--tRNA ligase", "db_xrefs": [ "COG:COG0018", "COG:J", "EC:6.1.1.19", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420", "KEGG:K01887", "SO:0001217", "UniRef:UniRef50_P47618", "UniRef:UniRef90_P75222" ], "nt": "TTGAAGACTACGCTCTATGGTGATGATAAGGACCGGGTGTTAGTGCGTCAAGACAATCGGCCTTCTTACTTTGGTACCGATGTCGCTTACCACTTAGACAAGGCTGCGCGGGGCTTTGACCTCTTATACGACATTTGGGGCAGTGATCATGAAGGGCACATTAAACGGATGCACTGTGTTTATGAGGGACTAAAGATTCACCAAAAATGCCAGCTCAAAATTACTGCCTTACAGTTGGTAATGCTGTACAAGAATAAGGAAATAGTCAGATTGTCCAAACGTGCTGGCAACGTTATTACCATTAAGCAAATGTTGCAAATGCTCAGTGAGGATGCGGCGCGTTGGTTTATGTTGTCGCAAACCAACAACTCGATTATCAAGATTGATTTGGACACTGCTAACCTGCAAAACTCTTCCAATCCGGTTTATTACGTGCAATATGCGTATGCCAGAATGTGCAGTGTATTAAAGGTAGTGGATCAAGCAGCACTAGCTCAAGTAAATGACTGCAGCTTATTAACGCATGAAAAGGAAATTGCTTTACTAGACCAGTTAGTGTACTTTAAGAGCTTACTGGAAAAAGTTCAGGTTAGCCATGAACTGCACCTCTTAACTAATTACTTATACGAGACAGCTACCTTGTTTCATTCTTGGTATAAGGCTTGCAAAATTAACGATCCAGCGCAGTACAATTTAACCCAGCAACGCTTACTTTTACTGCAATCATTGCACCATGTTTTTGGTCAGCTATTGCAAATCTTAAACATTACTGCACCACAACAAATGTAA", "aa": "MKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM", "aa_hexdigest": "d3a64c3b9aaab12963d011da547d34d9", "start_type": "TTG", "rbs_motif": null, "genes": [ "argS" ], "truncated": "3-prime", "pseudogene": { "start": 670374, "stop": 671462, "observations": { "mutation": [ 671165 ], "stop": [ 671165 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75222", "query_cov": 1.0, "subject_cov": 0.48789571694599626, "identity": 0.996, "score": 520.0, "evalue": 4e-182, "gene_start": 1, "gene_end": 262, "reference_start": 276, "reference_end": 537, "reference_sequence": "MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDKLSFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAIWLKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM" }, "paralog": false, "identity": 0.9917127071823204, "score": 723.0, "evalue": 2.22e-264, "description": "Internal stop codon at 671165. Nonsense mutation around 671165.", "psc": { "uniref90_id": "UniRef90_P75222", "gene": "argS", "product": "arginine--tRNA ligase", "ec_ids": [ "6.1.1.19" ], "uniref50_id": "UniRef50_P47618", "cog_id": "COG0018", "cog_category": "J", "kegg_orthology_id": "K01887", "go_ids": [ "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75222", "EC:6.1.1.19", "UniRef:UniRef50_P47618", "COG:COG0018", "COG:J", "KEGG:K01887", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ] }, "pscc": { "uniref50_id": "UniRef50_P47618", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47618" ], "product": "Arginine--tRNA ligase" } }, "id": "CNOCLNGIAL_1191", "locus": "GCA000143945_01190" }, { "type": "cds", "sequence": "contig_1", "start": 671163, "stop": 671987, "strand": "-", "frame": 1, "gene": "argS", "product": "arginine--tRNA ligase", "db_xrefs": [ "COG:COG0018", "COG:J", "EC:6.1.1.19", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420", "KEGG:K01887", "SO:0001217", "UniRef:UniRef50_P47618", "UniRef:UniRef90_P75222" ], "nt": "ATGCTTTTCATTAACACAGATCTTCAAGAATGTCTTAACGCTTTGAATCTAGAATTTGATGAGCACAAAGAGTTAGTCAAACTTGTTAAAAACAACAGCTTTAGTGGTTTTGCTTCCACCGTTGTGTTCCACTTAAAGGGTGTAAATCAAAAGGAAACCGCCCAACAGATTGCTGCTTGGTTGTTAAAACACAAAAAGGCGCATTACCGCCGTGTGTTTGTCGCTAATAACAACTTCATTAACTTTGAAATTAGCCCTCAAAAATACCTCGATTTTTTAAAAACCAAACCTACTTTTGCCCCTAAACCAACTAAGGTTCTAATTGAATGGGTGTCGGCTAACCCCACGGGTGAGTTGCACTTAGGTCATGTCCGCAATGCCTTCTTTGGTCATGTACTCAATAACCTGATGGTTTTTTTAGGCTTTCAAACAGTTCGGGAATACTGGGTCAATGACTATGGTCAACAAGCACGGGTGTTTGGTTTTAGTGTGTACCAAGCACTCCACTTACAACAAAACATTAAGGTCACTCCTCACCCTGATGGTTATGAGGGGGAGCTAGTGGACAGTATTGCCAAAACTATTACCGGTATTCCTTTAGATAACATCAGCTTTGAAGAGTTTTTACAACAACCCTTTCTGGACCAGTTACTAGCTGACTGTACTGCCAAAGTCTTGGAAGTAATTAAGCAAGACTTAGCTACAATCCACATTCACTTTGACAGCTGGAAGTTTGAAAGTCAAGTGGTTAAGGAAACAGATTACAAAAAACTGTTAACCCAGTTTAAGGACGAAGCGCACTATGAAAAGGATGGTGCCATTTGA", "aa": "MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDNISFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAI", "aa_hexdigest": "d647fd5d485d3172661a27ac2facc7e1", "start_type": "ATG", "rbs_motif": null, "genes": [ "argS" ], "truncated": "5-prime", "pseudogene": { "start": 670860, "stop": 671987, "observations": { "mutation": [ 671165 ], "stop": [ 671165 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75222", "query_cov": 1.0, "subject_cov": 0.5102420856610801, "identity": 0.993, "score": 552.0, "evalue": 7.94e-195, "gene_start": 1, "gene_end": 274, "reference_start": 1, "reference_end": 274, "reference_sequence": "MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDKLSFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAIWLKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM" }, "paralog": false, "identity": 0.992, "score": 760.0, "evalue": 6.2e-279, "description": "Internal stop codon at 671165. Nonsense mutation around 671165.", "psc": { "uniref90_id": "UniRef90_P75222", "gene": "argS", "product": "arginine--tRNA ligase", "ec_ids": [ "6.1.1.19" ], "uniref50_id": "UniRef50_P47618", "cog_id": "COG0018", "cog_category": "J", "kegg_orthology_id": "K01887", "go_ids": [ "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75222", "EC:6.1.1.19", "UniRef:UniRef50_P47618", "COG:COG0018", "COG:J", "KEGG:K01887", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ] }, "pscc": { "uniref50_id": "UniRef50_P47618", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47618" ], "product": "Arginine--tRNA ligase" } }, "id": "CNOCLNGIAL_1192", "locus": "GCA000143945_01191" }, { "type": "cds", "sequence": "contig_1", "start": 672015, "stop": 673853, "strand": "+", "frame": 3, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "COG:COG0445", "COG:J", "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660", "KEGG:K03495", "RefSeq:WP_010874914.1", "SO:0001217", "UniParc:UPI000012B445", "UniRef:UniRef100_P75221", "UniRef:UniRef50_P75221", "UniRef:UniRef90_P75221" ], "nt": "ATGTCTTTTACACTAACAGTTATTGGTGGCGGTCATGCCGGATTAGAGGCCGCATTTATTGCTAGTAAACTAGGGCTAAAAGTTAACCTCTTAGTACTAGATCCCAACCATGTGGGGTCGTGTCCCTGTAACCCGGCCATAGGAGGTCCTGCTAAAGGTATAGTAACCCGGGAAATCGATGTTTTAGGAGGGATGCAGGGTAAGGCCGCTGATGCCACTGCTTTACAATACAAGCTGCTCAACAGCTCGAAAGGTCCAGCAGTGCAAGCAATCCGCGCTCAAATAGACAAAATTGCTTATCAGAAGTGGTTTCGCCAGCAAATTGACCAAACGCCTAACATTGAGTTAATTGCTGGGGAAGCCGTTGATATCCTTGAAAGTAACGGTAAAGTTAAAGGTGTCGTGTTAGCTGATGGTAGTGAACTGGCATCAGATGCTGTTATTGTGACTACGGGAACTTATCTAAAGGCCAAAACTTACTGTGGTAGCTTAAGCAAAGAAGAAGGTCCTGACCGCGCTAAGCGCAGTGAATACCTTTCCACTAACTTAATTAAGCGGGGTTTTAAAACGTTACGACTAAAAACTGGGACACCACCACGGATCCTACGCGAATCACTCGACTTTAGCCAAATGGCTGTGGAAGCTAACACTACCCCTCATTTAGCCTTTAGCTTTACTACTAAGAATTATTTACCACTAGAACAGCAAGTGATCTGTCACTTAATCCATACCAATCCCCAGATCCATCAGTTAATTCTGGCCAACTTAAAACAGTCCGCTGTCTTTAACGGTAGTATTAAAGCAAACGGACCACTTTACTGTCCGAGTATTGAAGACAAGGTATTTCGCTTTCAAGACAAAGAACGGCACCAAATCTTTGTCGAACCTGAATCACTTAGTTTAGAAACGGTTTACCTAGCAGGGTTTTCCACTTCCTTTCCCCCAGAGGTGCAAGAGCACATTGTCCGTTTACTCCCTGGGTTTAAAAATGCCCGGTTTCAAAAGTATGGCTATGCCATTGAATATGATGCCTTTAGTTCGATACAACTCAAATCAACACTAGAAACTAAGTTAATTCAAAATCTGTATTTTGCCGGACAGATTAATGGTACCAGTGGGTATGAGGAAGCAGCTGGCCAGGGCTTAATCGCTGGGATTAACGCCGCTTTAAAGCTACAGCGTAAACCCGAATTTGTGTTACAGCGCAATGAGGCATACCTTGGTGTCATGATTAACGACCTGGTTACTAAGGAAATTAGTGATCCCTACCGCTTGTTAACTTCGCGCGCTGAACACCGTTTGTGGTTGCGCAATGATAACTTACAAGAACGTCTAATTGAAAAGAGCCGAGCCTTAGGTTTAGTGGAAGCAGATGTGTATGCCAATTACTTGGAACAACAACAAAAGAAAAAACAATTAATTGATTATTTGCAAACCACTACCGTTGGGCAAATTGCTGCCTTGAAATTAAACTTTAAAAACACGGCCCAAACGCTGTTTGACTTTACCAAGCGTGCTGAAATTAAGCTGGTTGATTTAGTACAACTACTCCCCAAGCGTTTTGACTTGGATGTACAAAGTTTAAACCAGATTGACATTGACATTAAGTACGCTGGTTACATTAAGAAGAGTGAAAAGTACTTCAAGAGTTTAAACAACCTCAGTAGTGTCAAAATTCCCTTAAAGTTAAATTACCACAAAGTACCTAACTTAGCGAGTGAAGCAATTGTTAAACTCTCCAAAATTAGACCAACTGATCTCAGTGTAGCTAGTCAAGTAGCGGGCATTAACTTTAACGATATTTTAGCGATTAAACATTTTTTAGATAACCATGAATAA", "aa": "MSFTLTVIGGGHAGLEAAFIASKLGLKVNLLVLDPNHVGSCPCNPAIGGPAKGIVTREIDVLGGMQGKAADATALQYKLLNSSKGPAVQAIRAQIDKIAYQKWFRQQIDQTPNIELIAGEAVDILESNGKVKGVVLADGSELASDAVIVTTGTYLKAKTYCGSLSKEEGPDRAKRSEYLSTNLIKRGFKTLRLKTGTPPRILRESLDFSQMAVEANTTPHLAFSFTTKNYLPLEQQVICHLIHTNPQIHQLILANLKQSAVFNGSIKANGPLYCPSIEDKVFRFQDKERHQIFVEPESLSLETVYLAGFSTSFPPEVQEHIVRLLPGFKNARFQKYGYAIEYDAFSSIQLKSTLETKLIQNLYFAGQINGTSGYEEAAGQGLIAGINAALKLQRKPEFVLQRNEAYLGVMINDLVTKEISDPYRLLTSRAEHRLWLRNDNLQERLIEKSRALGLVEADVYANYLEQQQKKKQLIDYLQTTTVGQIAALKLNFKNTAQTLFDFTKRAEIKLVDLVQLLPKRFDLDVQSLNQIDIDIKYAGYIKKSEKYFKSLNNLSSVKIPLKLNYHKVPNLASEAIVKLSKIRPTDLSVASQVAGINFNDILAIKHFLDNHE", "aa_hexdigest": "60eda10628024d38f59690539339f17b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B445", "ncbi_nrp_id": "WP_010874914.1", "uniref100_id": "UniRef100_P75221", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B445", "RefSeq:WP_010874914.1", "UniRef:UniRef100_P75221" ] }, "ips": { "uniref100_id": "UniRef100_P75221", "uniref90_id": "UniRef90_P75221" }, "psc": { "uniref90_id": "UniRef90_P75221", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_P75221", "cog_id": "COG0445", "cog_category": "J", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75221", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75221" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" }, "genes": [ "mnmG" ], "id": "CNOCLNGIAL_1193", "locus": "GCA000143945_01192" }, { "type": "cds", "sequence": "contig_1", "start": 673846, "stop": 674421, "strand": "+", "frame": 1, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "COG:COG0357", "COG:J", "EC:2.1.1.-", "EC:2.1.1.170", "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043", "KEGG:K03501", "RefSeq:WP_010874915.1", "SO:0001217", "UniParc:UPI000012B477", "UniRef:UniRef100_P75220", "UniRef:UniRef50_P75220", "UniRef:UniRef90_P75220" ], "nt": "ATGAATAACAGTAAATTGAAACAGTATGTTCAACTAGTACAAACAGCCAACCAAAACTTTAACCTCACCGGTCTTAAAACTGAAGGCGAAATTTACGAACACTTAGTCCAAGAAATTATCGAGCTCTTTAATGAGTATGACAGTTACTTTGACCATAAAAAGGTTGCAGATTTGGGTAGTGGAAATGGTTGTCCTGGTGTCATCTTAAAGCTGTTGTTTCCCCAAATCAAAACTTTGGATTTAATTGATAGCAAACACAAAAAGGTTAACTTTTTAAAAGAGGTGATTCAAACACTGGAACTAAACAATACCCAAGCACTTTGTGCCAGAATTGAAAACCACACTGAACAATACGACACTTTATGCTCCCGCGGACTTGGCAGCATTATTGAGGTTAATGCCTTTGCTTTAAAACTGTTAAAGCCCAACGGCATTATCTTCCACATTAAACAAAGCTTAGATCAATACCTCGAGTTTGAGGATTCGGAGCAAAAAGACCAGTTTAAACCACTGTTCTTTAAGTTTTTCCATGGCAAGCGCCAGCAAATCCTGATTGCCATGAAGAAAAATGTTTAA", "aa": "MNNSKLKQYVQLVQTANQNFNLTGLKTEGEIYEHLVQEIIELFNEYDSYFDHKKVADLGSGNGCPGVILKLLFPQIKTLDLIDSKHKKVNFLKEVIQTLELNNTQALCARIENHTEQYDTLCSRGLGSIIEVNAFALKLLKPNGIIFHIKQSLDQYLEFEDSEQKDQFKPLFFKFFHGKRQQILIAMKKNV", "aa_hexdigest": "be8301c57ed36447e490b8d2609dca06", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B477", "ncbi_nrp_id": "WP_010874915.1", "uniref100_id": "UniRef100_P75220", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B477", "RefSeq:WP_010874915.1", "UniRef:UniRef100_P75220" ] }, "ips": { "uniref100_id": "UniRef100_P75220", "uniref90_id": "UniRef90_P75220" }, "psc": { "uniref90_id": "UniRef90_P75220", "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.-", "2.1.1.170" ], "uniref50_id": "UniRef50_P75220", "cog_id": "COG0357", "cog_category": "J", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043" ] }, "pscc": { "uniref50_id": "UniRef50_P75220", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75220" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "CNOCLNGIAL_1194", "locus": "GCA000143945_01193" }, { "type": "cds", "sequence": "contig_1", "start": 674471, "stop": 674623, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTGCCAAACAGTTTTATGTAACTAGTAGTGCTGGTGCAATTCTGTTTGACCTTAAAAAGAGTCAGGGCTTAGACCAACTGCAAGCAATTGAAAAAACAAGGAAGGTTATTATTACAGAACAGTTTGCCAACCAAACTGGTAAATGA", "aa": "MEFAKQFYVTSSAGAILFDLKKSQGLDQLQAIEKTRKVIITEQFANQTGK", "aa_hexdigest": "71181d2970941635dc4be202fd1bff47", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5622.407499999999, "isoelectric_point": 9.22402362823486 }, "id": "CNOCLNGIAL_1195", "locus": "GCA000143945_01194" }, { "type": "cds", "sequence": "contig_1", "start": 674627, "stop": 674848, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGACAAGGAAAACACTAAACGGATTAACAGCTTAGCGCAAGAGCACATTACTCCCTTAATAAAACGGATTTTACGCTTAGCGGACTTTAAAAATGTCTTGATTAATGTGCAGCACCACAAGAAGCTGCAAAAATGTCTGTTATGGGAAATTAACGGTTTAATCTGCTTAGTTGAAAGTTTACAGTTCATGGAAAATCCCACTGCAATCATGGAATGA", "aa": "MFDKENTKRINSLAQEHITPLIKRILRLADFKNVLINVQHHKKLQKCLLWEINGLICLVESLQFMENPTAIME", "aa_hexdigest": "907136105473540eec5b93e8c71ce90e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8614.161799999998, "isoelectric_point": 8.712273597717285 }, "id": "CNOCLNGIAL_1196", "locus": "GCA000143945_01195" }, { "type": "cds", "sequence": "contig_1", "start": 674845, "stop": 675081, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCCAGGGATTAAAAAACACTGTCCTAATGTAGCAGTTGTAACCATTAGTGGTCAACACAAACCAGTCATAGAACCGAGCTTAACCGAATATAAGGCAGTATTTGGTAGTAGTTTACTCTCCTTTCACTTAGATGCAACTACGATAAATAACTCCCATTTAGTGCGTCAAATTTTGGAACAGATCAAAATAAAAGCTACCTTAAAGTCAAACTCTAAGGTAGCTAAAAGTTAA", "aa": "MIPGIKKHCPNVAVVTISGQHKPVIEPSLTEYKAVFGSSLLSFHLDATTINNSHLVRQILEQIKIKATLKSNSKVAKS", "aa_hexdigest": "35c6aa5e3c10cc905fe8267fa46a3ac8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8506.896399999994, "isoelectric_point": 9.845498847961426 }, "id": "CNOCLNGIAL_1197", "locus": "GCA000143945_01196" }, { "type": "tRNA", "sequence": "contig_1", "start": 675082, "stop": 675157, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 74.8, "nt": "GCGTCGGTAGCTCAGCGGATAGAGTACACGCCTTCTAAGTGTGTTGTCGTGGGTTCGAGTCCCACCCGATGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 675122, 675124 ], "id": "CNOCLNGIAL_17", "locus": "GCA000143945_01197" }, { "type": "cds", "sequence": "contig_1", "start": 675159, "stop": 676475, "strand": "-", "frame": 1, "gene": "arcA", "product": "Arginine deiminase-like protein", "db_xrefs": [ "COG:COG2235", "COG:E", "GO:0016990", "GO:0019546", "RefSeq:WP_010874917.1", "SO:0001217", "UniParc:UPI0000125DB1", "UniRef:UniRef100_P75218", "UniRef:UniRef50_P75218", "UniRef:UniRef90_P75218" ], "nt": "ATGTCAAAAAAACAGCTTGTCAAAACCGATGGGCATAACCAATTAGATCAACCAAACACTAAAGCACTACAGTTAAAAAAGAAGCAGTTTAACAGTGGTGTACGCGTTACTAGTGAAATTAGTTTTTTAAGGGAAGTAATTGCCCATCATCCCGGTATCGAAACCGAACGAGTGATTGATAACCAGACCTTTGGCAGTGCAATGTACTTAGAACGCGCTCAAAAGGAACACCAGTTATTTATTAAGATTTTGCGCCAACATGGTACAAAGGTGCACTATTTACAAGATTTACTGTTAGAAGCTTTAAGTGCGGCTGACCCTAATGTGCGCCAGGACTTCATTAAGAACTTTTTATTGGAATCAGGCATTAAAAGTGTGAGTACCTTCGAAGCTTGCTTAAACTTCTTTCGCAGTTTAGATTCGCTTGTAGATGTGATTAAGGTCATGTTTGGTGGCATTAAGGTATCAGATGTACCACCGATTACCCCGCAACGGTTTGCCGACATTCATGTAAGTAATTCACCGTTTTTAATTAAACCGCTATCGTTTTCCCTTTATCCACATAAGTTCTTCAATACCCTAGGAACGGGTGTCGCTTTATTTGTCACTAATGATTCTGAGTTAAAGCGCCATTCGTTAGTGTATGAATACATCATGCGCTTTCACCCCCGTTTTGATGGAGTTAAGCTTTATACTAACCGTGACTTTAAGAACTGTTTGATTAACAGTAGTGACATTATTCAAATTAGCAATGAAATCTTGCTAATTGGTATTAGTCATGACACCGATGTGTTGGGAATTGAAAGCTTGGCACGTAATTTATTAAGCGATCACACTAATCCAATTAAGCAGATTATTGCAATTAATATCCACAAGTTTGGTGCTAAGACCAATCTCAATAAACTTATAGCGATGGTGGATGTCGACAAGTTCATTATTGCGCGCAAAGTGTTACAAGCTACCGAAATCTTTGAACTCACAGCCACCGCCCAGAGGGATGTGGATGGCTTAGCACAAATTAAATTCAAACCGCTAAAGTTTAATTTTGGTGAGATAATTGAAGCGATTATTGATAAACAACCCCGCTTTGTTATTATTGGTGGTGGTGATGAAGTGGCTGAGCGTAAAGAACTCTTGGACTGTGGTATGGGCGTATTAAACTTAAGCCCTGGAGAAATTGTGGTCTTTGATCGCAACCACTACACCAATAATCTTCTCAATGAACTGGGATTAATCATCCATAAGATCCCCGCTTCCGAACTTTCACGGGGTCCTAGTGGCCCCTTGGAAATGGTTTGCTCTCTCTGGAGAGAATAA", "aa": "MSKKQLVKTDGHNQLDQPNTKALQLKKKQFNSGVRVTSEISFLREVIAHHPGIETERVIDNQTFGSAMYLERAQKEHQLFIKILRQHGTKVHYLQDLLLEALSAADPNVRQDFIKNFLLESGIKSVSTFEACLNFFRSLDSLVDVIKVMFGGIKVSDVPPITPQRFADIHVSNSPFLIKPLSFSLYPHKFFNTLGTGVALFVTNDSELKRHSLVYEYIMRFHPRFDGVKLYTNRDFKNCLINSSDIIQISNEILLIGISHDTDVLGIESLARNLLSDHTNPIKQIIAINIHKFGAKTNLNKLIAMVDVDKFIIARKVLQATEIFELTATAQRDVDGLAQIKFKPLKFNFGEIIEAIIDKQPRFVIIGGGDEVAERKELLDCGMGVLNLSPGEIVVFDRNHYTNNLLNELGLIIHKIPASELSRGPSGPLEMVCSLWRE", "aa_hexdigest": "779244268347b9d84ffb03fd1fa41faa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125DB1", "ncbi_nrp_id": "WP_010874917.1", "uniref100_id": "UniRef100_P75218", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125DB1", "RefSeq:WP_010874917.1", "UniRef:UniRef100_P75218" ] }, "ips": { "uniref100_id": "UniRef100_P75218", "uniref90_id": "UniRef90_P75218" }, "psc": { "uniref90_id": "UniRef90_P75218", "gene": "ArcA", "product": "Arginine deiminase-like protein", "uniref50_id": "UniRef50_P75218", "cog_id": "COG2235", "cog_category": "E", "go_ids": [ "GO:0016990", "GO:0019546" ] }, "pscc": { "uniref50_id": "UniRef50_P75218", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75218" ], "product": "Arginine deiminase-like protein" }, "genes": [ "arcA" ], "id": "CNOCLNGIAL_1198", "locus": "GCA000143945_01198" }, { "type": "cds", "sequence": "contig_1", "start": 676529, "stop": 676735, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00485.24" ], "nt": "ATGGATAGTAAAAAAGGAATTTTAGTGGCAATTGGTGGCGGGAGTTGTTCTGGTAAAACTACGATCGCTGACATGATTTACCAACTTTTGCGTAAAAAGCTTAAAGTTGCTATTCTGCCCCAGGACAACTATTACAAACCATACAAGAACAAATCAATGGCGCAACGCAAAGCGATTAACTTCGATCACCCCGATGCCTTTGATTGA", "aa": "MDSKKGILVAIGGGSCSGKTTIADMIYQLLRKKLKVAILPQDNYYKPYKNKSMAQRKAINFDHPDAFD", "aa_hexdigest": "e1ab7c080a2409d72ea9958a36166eff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00485.24", "name": "PRK", "length": 69, "aa_cov": 0.8823529411764706, "hmm_cov": 1.0, "evalue": 6.70131174315273e-07, "score": 24.76234245300293, "start": 9, "stop": 68 } ], "seq_stats": { "molecular_weight": 7606.822200000001, "isoelectric_point": 9.629658699035645 }, "id": "CNOCLNGIAL_1199", "locus": "GCA000143945_01199" }, { "type": "cds", "sequence": "contig_1", "start": 676769, "stop": 677014, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00485.24" ], "nt": "ATGGGTAAAACAGTGGCTGTGCCAATGTATGACTATGTTAATTACACCCGTAAAAAGGAAACCATAGAAATAGGTCCTTTAAATGTAGTCATCTTGGAAGGTTTAATGCCGTGGTTTGATGAACGGATTGCTAAATTGTGTAAGCTCAAGATCTTTGTGGAAGCGACTGGAGAGGAACGCTTAATTCGCCGGATTGAACGGGACTGGGAACGCGGTCGTGATGTCGCTTCTATCATTAAACAATGA", "aa": "MGKTVAVPMYDYVNYTRKKETIEIGPLNVVILEGLMPWFDERIAKLCKLKIFVEATGEERLIRRIERDWERGRDVASIIKQ", "aa_hexdigest": "77dc1989918fd2cfa2d3ab0aab7eea23", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00485.24", "name": "PRK", "length": 81, "aa_cov": 0.9876543209876543, "hmm_cov": 1.0, "evalue": 2.7595582170289286e-18, "score": 61.91554260253906, "start": 2, "stop": 81 } ], "seq_stats": { "molecular_weight": 9540.122499999992, "isoelectric_point": 8.816132164001466 }, "id": "CNOCLNGIAL_1200", "locus": "GCA000143945_01200" }, { "type": "cds", "sequence": "contig_1", "start": 677033, "stop": 677170, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGAAATCTTTGTCGAAAAGATGAAACAGAAGGCAGATTTAATTATTCCCTGGAGTGAAAGACACGAAGTGAGTACTAATGTGCTCGACTTTGCAATTGAACACCTCTTTCGCAAACACGTGGATCCCAACTAG", "aa": "MYEIFVEKMKQKADLIIPWSERHEVSTNVLDFAIEHLFRKHVDPN", "aa_hexdigest": "6d882a79fb617e52dbfdf00aca6704cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5455.228799999999, "isoelectric_point": 5.80777416229248 }, "id": "CNOCLNGIAL_1201", "locus": "GCA000143945_01201" }, { "type": "cds", "sequence": "contig_1", "start": 677167, "stop": 677832, "strand": "-", "frame": 3, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "COG:COG0171", "COG:H", "EC:6.3.1.5", "GO:0003952", "GO:0004359", "GO:0005524", "GO:0005737", "GO:0008795", "GO:0009435", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47623", "UniRef:UniRef90_P75216" ], "nt": "GTGGTTGTTGGATTGTCAGGTGGAGTTGATTCGGCAGTTGTAGCCGCCATGGCTAAAAAACTGTTTCCGCAAAACCACTTAACCTTGGTAATGCACATTAATAACTCCGAGTTAGATCACAAGGCCACAACTGCCTTAGTGGAACAATTACAACTCAACAATAAACAGGTGGATTTAGAACCACCTTACCGCGCTATGTTGCAAGCATTGACCATTGATCCCCAAAAGGAGTTAATGGTAGCCGGCAACCTCAAAGCACGTTTACGAATGGCTTGTCTATACACCCATGCCCAAAAGCACAACTACTTAGTTTTGGGTACTGGCAACTTCATTGAGTACAGCTTGGGCTACTTTACTAAATGGGGTGATGGGGCGTGTGATGTGGCACCCTTAGCGTTCTTGTTAAAGAGCGATGTTTATGCTTTATCCCAGCACTTCAATGTTCCTGAATTGGTCATTGAACGAGCCCCTACTGCTAGTCTGTTTGCCGGGCAGACTGACGAAGCGGAAATGGGTTTGACGTATAAGGAATTGGACCAGTACTTTCAGGGCCACTTACAGTTAAGTGCCACGAAACAACAACGGGTTGACCACTTGCGCCAAAGCTCCCAGCACAAGCGTAGTTTACCTAAAACCTTTAAACCACTTTATTCCTTCCAAATCTAG", "aa": "MVVGLSGGVDSAVVAAMAKKLFPQNHLTLVMHINNSELDHKATTALVEQLQLNNKQVDLEPPYRAMLQALTIDPQKELMVAGNLKARLRMACLYTHAQKHNYLVLGTGNFIEYSLGYFTKWGDGACDVAPLAFLLKSDVYALSQHFNVPELVIERAPTASLFAGQTDEAEMGLTYKELDQYFQGHLQLSATKQQRVDHLRQSSQHKRSLPKTFKPLYSFQI", "aa_hexdigest": "c08c3536d2d321b9d2b7bff552278c12", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75216", "query_cov": 1.0, "subject_cov": 0.8911290322580645, "identity": 0.991, "score": 431.0, "evalue": 2.08e-152, "valid": true, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "ec_ids": [ "6.3.1.5" ], "uniref50_id": "UniRef50_P47623", "cog_id": "COG0171", "cog_category": "H", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0004359", "GO:0046872", "GO:0003952", "GO:0008795", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_P47623", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47623" ], "product": "NH(3)-dependent NAD(+) synthetase" }, "genes": [ "nadE" ], "id": "CNOCLNGIAL_1202", "locus": "GCA000143945_01202" }, { "type": "cds", "sequence": "contig_1", "start": 677906, "stop": 679072, "strand": "-", "frame": 2, "gene": "obgE", "product": "GTPase ObgE", "db_xrefs": [ "COG:COG0536", "COG:DL", "EC:3.6.5.-", "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_P75215", "UniRef:UniRef90_P75215" ], "nt": "GTGTTGCAAGCCGACCACAACTGCGATTCCCTCTTTTTTTTAAAGAATAAAAAACACCTGTTTGCTGAAAGTGGCGGCAATGGCAAACCCGATTTAGCCCATGGTAAAAACGGGGAAGATTTGGTTATTAAGGTACCAGTGGGAACTACGGTGCGTGACCTTGATACTAACCAGATCTTAATGGACTTTGTTCATGACCAACAGAGCTTTATTCTCTGTTACGGTGGCAAGGGCGGTAAGGGCAACGCAGCTTTTAAAAGTCCAATTATGCGCGCTCCCAATCTGTATGAAAACGGCGACAAGGGTCAAAGTTTGCATGTGAGCTTGGAAATAAAGTATTTAGCTAATGTGGGGATTGTGGGTTTTCCCAACACCGGTAAGTCCACTTTAATTTCCAAACTGTCCAACGCTAAACCCAAGATTGCTAACTACCGTTTTACAACCTTAGTGCCCGTATTGGGGGTAGTTAAACACAACGACCAAAGTTTGGTGTTTGCTGATATTCCCGGGTTAATTGAAAACGCCAGTGAGGGTAGTGGTCTGGGTCACTATTTCCTGCGCCACATTGAGCGTTGTGAAATCCTAATTCACTTAATTTCACTCGATCCCGTGGATCATGACGATCCTTGTCAAGCGTATGAACAAATTATGCGTGAACTGTCGAAGTACTCCCAGCTGTTAGTTAAAAAGAAGATGCTGGTCGTGGCCAATAAAACAGACGTTGACCTTGATGGTACCCGTTTTCAAAAGTTAGCACAATACCTCGAAAATAAAGGAATTCCCTTGTTCAAAATTAGTGCACTCAAACAAGAACTGGGTGATCTAGTGGCCCAAGTGTTTGCGCTCCACCAAAAAACGCTAGCGCAATTCGGTGCCAATAAGTTTCATTTACCCATGGAAATGGAGAAGCACTATGTCTTTGAACAGGCCAGTGAAACCGACCATGATCCACTCAACATTGAACGTGATGCCTTGGGGCGGTGGCATGTTGAATGCAAGCGTTTACATTATTGGTTTGACAAGATTCCCCAAACTACACTTGACAACATTCGCCGTTTAGGTAATAAGATTAAAGAAGTGGGCATAGAAGACCAATTAAAGGTGGCAGGTGCCAAAAAAGGCGATGTCATTGTGTTTGCTGGCCAGGAATTTGTAATTAATGACTAA", "aa": "MLQADHNCDSLFFLKNKKHLFAESGGNGKPDLAHGKNGEDLVIKVPVGTTVRDLDTNQILMDFVHDQQSFILCYGGKGGKGNAAFKSPIMRAPNLYENGDKGQSLHVSLEIKYLANVGIVGFPNTGKSTLISKLSNAKPKIANYRFTTLVPVLGVVKHNDQSLVFADIPGLIENASEGSGLGHYFLRHIERCEILIHLISLDPVDHDDPCQAYEQIMRELSKYSQLLVKKKMLVVANKTDVDLDGTRFQKLAQYLENKGIPLFKISALKQELGDLVAQVFALHQKTLAQFGANKFHLPMEMEKHYVFEQASETDHDPLNIERDALGRWHVECKRLHYWFDKIPQTTLDNIRRLGNKIKEVGIEDQLKVAGAKKGDVIVFAGQEFVIND", "aa_hexdigest": "7c5bbd50a3cee0623c8e3dc979003ce8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75215", "query_cov": 1.0, "subject_cov": 0.8960739030023095, "identity": 0.997, "score": 785.0, "evalue": 2.08e-286, "valid": true, "gene": "obgE", "product": "GTPase ObgE", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75215", "cog_id": "COG0536", "cog_category": "DL", "go_ids": [ "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75215", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75215" ], "product": "GTPase Obg" }, "genes": [ "obgE" ], "id": "CNOCLNGIAL_1203", "locus": "GCA000143945_01203" }, { "type": "cds", "sequence": "contig_1", "start": 679411, "stop": 679761, "strand": "-", "frame": 3, "gene": null, "product": "NADP-dependent alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A645H7Y1", "UniRef:UniRef90_A0AB38W5V0" ], "nt": "ATGGAAAAGGTGTTAATTTCTGGTGGTGGTTCGACTATCTTAGAAACAGCGATTAAGGTTTTAAGACCAGGAGGCAAAATTGGTAACGTTAATTACTTTGGCGCTGGTGAGTTTTTAACTATTCCTAGAGTTGAATGGGGCGTTGGTATGGCTCACAAGGCCATTCATGGTGGTCTTATGCTTGGTGGTCGGTTAAGGTTAGAAAAATTAGCACGTTTAATTATGACCAAAAAACTCGATCCTTCCAAAATGATTACCCATCGCTTTAAGGGTTTTGAACACATTGAAGAAGCCCTGTTTTTAATGAAAGATAAACCGAAGGACTTAATTAAATCCGTGGTTATTTTTTAA", "aa": "MEKVLISGGGSTILETAIKVLRPGGKIGNVNYFGAGEFLTIPRVEWGVGMAHKAIHGGLMLGGRLRLEKLARLIMTKKLDPSKMITHRFKGFEHIEEALFLMKDKPKDLIKSVVIF", "aa_hexdigest": "3dab30da03fae1a49e874498de88cc9e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002ED5893F", "query_cov": 0.9482758620689655, "subject_cov": 0.8148148148148148, "identity": 0.5820000000000001, "score": 139.0, "evalue": 1.88e-40, "valid": false, "uniref50_id": "UniRef50_A0A352UL42" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 679411, "stop": 680031, "observations": { "mutation": [ 679779, 679980, 679989 ], "stop": [ 679779, 679980, 679989 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W5V0", "query_cov": 1.0, "subject_cov": 0.5631067961165048, "identity": 1.0, "score": 230.0, "evalue": 2.22e-75, "gene_start": 1, "gene_end": 116, "reference_start": 91, "reference_end": 206, "reference_sequence": "MYVVRHDSHRLSRQWTSWHPIRCRVVVFGAGPVGLMAIAGAALKGAGRIIVVDSRPDIVEIAKAYGATDYIDFKKVSVVDEILKWTNNEGMEKVLISGGGSTILETAIKVLRPGGKIGNVNYFGAGEFLTIPRVEWGVGMAHKAIHGGLMLGGRLRLEKLARLIMTKKLDPSKMITHRFKGFEHIEEALFLMKDKPKDLIKSVVIF" }, "paralog": false, "identity": 0.9805825242718447, "score": 395.0, "evalue": 7.21e-143, "description": "Internal stop codon at 679779, 679980, 679989. Nonsense mutation around 679779, 679980, 679989.", "psc": { "uniref90_id": "UniRef90_A0AB38W5V0", "product": "NADP-dependent alcohol dehydrogenase", "uniref50_id": "UniRef50_A0A645H7Y1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W5V0", "UniRef:UniRef50_A0A645H7Y1" ] } }, "id": "CNOCLNGIAL_1204", "locus": "GCA000143945_01204" }, { "type": "cds", "sequence": "contig_1", "start": 679777, "stop": 679926, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00107.32" ], "nt": "ATGGCGATCGCTGGTGCAGCCTTAAAGGGTGCTGGACGCATTATTGTGGTGGATTCTAGACCAGATATTGTGGAAATTGCCAAAGCATACGGTGCGACTGATTACATTGATTTCAAAAAGGTTTCAGTTGTGGATAAAATCTTGAAGTGA", "aa": "MAIAGAALKGAGRIIVVDSRPDIVEIAKAYGATDYIDFKKVSVVDKILK", "aa_hexdigest": "afbaaeabd5719b45072d0ddd6e2a913f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00107.32", "name": "ADH_zinc_N", "length": 48, "aa_cov": 0.9795918367346939, "hmm_cov": 0.9791666666666666, "evalue": 2.3495170233425077e-09, "score": 32.74916076660156, "start": 1, "stop": 48 } ], "seq_stats": { "molecular_weight": 5191.0942000000005, "isoelectric_point": 9.156912040710449 }, "id": "CNOCLNGIAL_1205", "locus": "GCA000143945_01205" }, { "type": "cds", "sequence": "contig_1", "start": 679926, "stop": 680165, "strand": "-", "frame": 1, "gene": null, "product": "NADP-dependent alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B0EC62", "UniRef:UniRef90_A0AB38W6U1" ], "nt": "ATGCACTCCGGTGGCATGCTGGGTGGTTGGAAGTTTTCCAACGTTAAAGATGGCATGTTTGCTGAATACTTTCACGTCAATGATGCTGAGGGTAACTTAGCATTGATGCCAGAAGGGATGGATTTAGCTGATGCATGTATGTTGTCAGACATGATTCCCACAGGCTTTCACGCCAATGAACTAGCTGACATCCAATACGGTGTCGCGTTGTCGTTTTTGGTGCTGGTCCAGTTGGTTTAA", "aa": "MHSGGMLGGWKFSNVKDGMFAEYFHVNDAEGNLALMPEGMDLADACMLSDMIPTGFHANELADIQYGVALSFLVLVQLV", "aa_hexdigest": "c9b1a6cf83e7543b1ce617904b9f4554", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 679926, "stop": 680465, "observations": { "mutation": [ 680198, 680426 ], "stop": [ 680198, 680426 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W6U1", "query_cov": 1.0, "subject_cov": 0.441340782122905, "identity": 1.0, "score": 160.0, "evalue": 6.95e-49, "gene_start": 1, "gene_end": 79, "reference_start": 101, "reference_end": 179, "reference_sequence": "MKAYAMLKIGATGWIEKPRPVCGPNDAIIRPLAVAPCTSDVHTVWEGGIGERHNMVLGHEGCGVVDEVGSEVKSFKVGDRVLVAAITPEWNSVNAQAGYPMHSGGMLGGWKFSNVKDGMFAEYFHVNDAEGNLALMPEGMDLADACMLSDMIPTGFHANELADIQYGVALSFLVLVQLV" }, "paralog": false, "identity": 0.9888268156424581, "score": 361.0, "evalue": 2.38e-130, "description": "Internal stop codon at 680198, 680426. Nonsense mutation around 680198, 680426.", "psc": { "uniref90_id": "UniRef90_A0AB38W6U1", "product": "NADP-dependent alcohol dehydrogenase", "uniref50_id": "UniRef50_B0EC62", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W6U1", "UniRef:UniRef50_B0EC62" ] } }, "id": "CNOCLNGIAL_1206", "locus": "GCA000143945_01206" }, { "type": "cds", "sequence": "contig_1", "start": 680196, "stop": 680303, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08240.17" ], "nt": "ATGGTTTTAGGCCATGAGGGCTGTGGTGTTGTGGATGAAGTGGGGTCTGAGGTCAAAAGCTTTAAGGTTGGTGATCGCGTTTTAGTGGCAGCCATTACACCAGAATGA", "aa": "MVLGHEGCGVVDEVGSEVKSFKVGDRVLVAAITPE", "aa_hexdigest": "028f5933663f10cf6e1ec59bc2a80dd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08240.17", "name": "ADH_N", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 5.0690558095662456e-11, "score": 38.2827033996582, "start": 1, "stop": 32 } ], "seq_stats": { "molecular_weight": 3628.1351999999997, "isoelectric_point": 4.781830406188964 }, "id": "CNOCLNGIAL_1207", "locus": "GCA000143945_01207" }, { "type": "cds", "sequence": "contig_1", "start": 680745, "stop": 680912, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGTGTTGGTAGTGTTAATGCTGAGTTTCTTTGTAATTAATAAACTAACTAACTTAGTTTTACTACGTTCACGCTATGCCATGGTAGTAGCACAAGGACTGGTTCACACAGGTAAAGTCAAACAACAAAGCCAACAGGCTATGTCTGACATTAAAACGAAATGA", "aa": "MLVLVVLMLSFFVINKLTNLVLLRSRYAMVVAQGLVHTGKVKQQSQQAMSDIKTK", "aa_hexdigest": "87bf19a1e80e57d93829103f5e0d3f66", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6190.500700000004, "isoelectric_point": 10.65547962188721 }, "id": "CNOCLNGIAL_1208", "locus": "GCA000143945_01208" }, { "type": "cds", "sequence": "contig_1", "start": 680940, "stop": 681233, "strand": "-", "frame": 1, "gene": null, "product": "MPN565 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75213", "UniRef:UniRef90_P75213" ], "nt": "TTGGTCTTTGTCTACTACATCATTGTGTGGGTGTTATTAGGGGAAATTAATGCCTTAGGTTTTAACTTTGTCATTGCCCCAAATAACCGTACTTTAATGGGTAGGGAAGTAGAACCCATTTTTGATTTAGTACTACTGTGGTTCTTACTAGTGCCCTTAGTAGTTTATGCGTTGCTTTTACTGCTTTTATTCTTTACAACCCCATACCTAATTGTGGAGGCAATTCCCTTTTTTTATGGAATTGCTTTGATGATGATTAGCCTCTTTATGAGTGGTATGTTTCCTCAAGCTTGA", "aa": "MVFVYYIIVWVLLGEINALGFNFVIAPNNRTLMGREVEPIFDLVLLWFLLVPLVVYALLLLLLFFTTPYLIVEAIPFFYGIALMMISLFMSGMFPQA", "aa_hexdigest": "5c9b7a13f146b7f5678732a27546d35d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 680679, "stop": 681305, "observations": { "mutation": [ 680747, 680942, 681260 ], "stop": [ 680747, 680942, 681260 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75213", "query_cov": 1.0, "subject_cov": 0.46634615384615385, "identity": 0.99, "score": 164.0, "evalue": 1.07e-49, "gene_start": 1, "gene_end": 97, "reference_start": 25, "reference_end": 121, "reference_sequence": "MEQFFQSTAKRKTPAWLTGSFSFFLVFVYYIIVWVLLGEINALGFNFVIAPNNRTLMGREVEPIFDLVLLWFLLVPLVVYALLLLLLFFTTPYLIVEAIPFFYGIALMMISLFMSGMFPQAWNVWVIFGRFVLVLVVLMLSFFVINKLTNLVLLRSRYAMVVAQGLVHTGKVKQQSQQAMSDIKTKWDKEKSKTVVVTIKKKRVKSSD" }, "paralog": false, "identity": 0.9807692307692307, "score": 376.0, "evalue": 2.17e-135, "description": "Internal stop codon at 680747, 680942, 681260. Nonsense mutation around 680747, 680942, 681260.", "psc": { "uniref90_id": "UniRef90_P75213", "product": "MPN565 family protein", "uniref50_id": "UniRef50_P75213", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75213", "UniRef:UniRef50_P75213", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75213", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75213" ], "product": "Uncharacterized protein MG384.1 homolog" } }, "id": "CNOCLNGIAL_1209", "locus": "GCA000143945_01209" }, { "type": "cds", "sequence": "contig_1", "start": 681307, "stop": 682020, "strand": "-", "frame": 3, "gene": "ugpQ", "product": "putative MG385-like protein", "db_xrefs": [ "COG:COG0584", "COG:I", "GO:0006629", "GO:0008081", "RefSeq:WP_014325603.1", "SO:0001217", "UniParc:UPI0001DD6431", "UniRef:UniRef100_A0AAV5N9Y2", "UniRef:UniRef50_P47625", "UniRef:UniRef90_P75212" ], "nt": "ATGCGCAAACAGTTTTTAATTGCACACCGTGGTTACAGCACCATTGCTCCTGAAAACACTCATTTAGCCTTTGCTGCTGCACAATTGTTTGGTTTTGATGGTTTGTGGATGGAAGTGCAACTCACTAAAGATAAACAAATAGTAGTCACTAATTTAGACAACTATAAGGTAGGAAATAAAACCCATAAACTGAGTGATATTAACCTAGTTGATCTCAAAAAGATTAACTTAGCGAAGCAGTTTAAGGTAAATGTCCAAGAACAGACAATCTTGACCTTAAAAGAGGTGTTAATGGAATTTTTAACCCCGTTTCGCTTTCTCTTGCTTTTTATTAAGGGCGAAGAAGAACAACAAAACCAGGTACTAGTAGAACAGTTAGCACAACTACTAGAAGGTTTTGAGTTAGCTAAAGAAAAGTTAATCTTGCTGTCCAGTCACTTTTCCACGATTAAGTACCTCAACGAAAAGTTAAAGAGCTTTAAAACTAGTTTTGTCTTTAACAGTAAAAAGCAGTTAGTTCATTTAACTAAAGATGAGATTACCCAGAACTGTCGTTTCTTAGCGCCCAATGATAGCTTTTACCACAAAAATTGTGCGGAGCTACAAAGCTATGGCCTGCCAATTATCCTCTGGGTAATTAAAGGGCTTTTACGTTACCAGTTTTACGAAAACGATCGCTTTGTTAAATTTCAAATAGCAGCACAACTTTACTAA", "aa": "MRKQFLIAHRGYSTIAPENTHLAFAAAQLFGFDGLWMEVQLTKDKQIVVTNLDNYKVGNKTHKLSDINLVDLKKINLAKQFKVNVQEQTILTLKEVLMEFLTPFRFLLLFIKGEEEQQNQVLVEQLAQLLEGFELAKEKLILLSSHFSTIKYLNEKLKSFKTSFVFNSKKQLVHLTKDEITQNCRFLAPNDSFYHKNCAELQSYGLPIILWVIKGLLRYQFYENDRFVKFQIAAQLY", "aa_hexdigest": "aea43eab7ddd6825a501ca6f9e39b8ad", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6431", "ncbi_nrp_id": "WP_014325603.1", "uniref100_id": "UniRef100_A0AAV5N9Y2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6431", "RefSeq:WP_014325603.1", "UniRef:UniRef100_A0AAV5N9Y2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9Y2", "uniref90_id": "UniRef90_P75212" }, "psc": { "uniref90_id": "UniRef90_P75212", "gene": "UgpQ", "product": "Uncharacterized protein MG385 homolog", "uniref50_id": "UniRef50_P47625", "cog_id": "COG0584", "cog_category": "I", "go_ids": [ "GO:0008081", "GO:0006629" ] }, "pscc": { "uniref50_id": "UniRef50_P47625", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47625" ], "product": "Uncharacterized protein MG385" }, "genes": [ "ugpQ" ], "id": "CNOCLNGIAL_1210", "locus": "GCA000143945_01210" }, { "type": "cds", "sequence": "contig_1", "start": 682416, "stop": 682532, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCATTATGAACCTAGTGGGGAATGGGTATGAGCTGGTTACTTTGATGACAACCAAATATGAACACCAGATGCTAGTGTTGAATGGGCACGTGAAAGTGATTACACAGATCTAA", "aa": "MVIMNLVGNGYELVTLMTTKYEHQMLVLNGHVKVITQI", "aa_hexdigest": "8f59a77be459679adb3303dbbee94c4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4332.179400000001, "isoelectric_point": 6.696679115295409 }, "id": "CNOCLNGIAL_1211", "locus": "GCA000143945_01211" }, { "type": "cds", "sequence": "contig_1", "start": 682558, "stop": 682962, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACGATTCCTGAACCAGAATCAATTGAAACTACTGAACCAGAACCTAATTTTGAACCAGAAGTAGAGCTTGAACCAGAAATTGAACCTAATTTCGAATCCGAAACTGAAGTTCAACAAGAGCTAGCACAAGAATCAAGTTTCGAGTCTGAACCAGAACCTAATTTTGAAACAGAAGTAGAAGTTCAACCTGAATCAGAAATTGAATTTAAATTCGAAGCCGAAGTTCAATCAGAGCCAAAAGTAAGTTTGAATTCAGATTTTGAAACTAAACCAGAAGCTCAAGCGGAGGTAACGCCAGAAACCTTGGAAGTAGAGGCAACATCTGAAGCTCCAGAACTTCAACCAGAAACTGAAGCAACAAAAGTAGTTGATGACGTTGAAGAAGAGCAACTTGATTGA", "aa": "METIPEPESIETTEPEPNFEPEVELEPEIEPNFESETEVQQELAQESSFESEPEPNFETEVEVQPESEIEFKFEAEVQSEPKVSLNSDFETKPEAQAEVTPETLEVEATSEAPELQPETEATKVVDDVEEEQLD", "aa_hexdigest": "c1d14925fd733753e610126553aa4a74", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15215.904899999992, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_1212", "locus": "GCA000143945_01212" }, { "type": "cds", "sequence": "contig_1", "start": 683140, "stop": 684387, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16713.10" ], "nt": "GTGTGGAAGGGTTTCTTTGACACCAAACTCAATTGGATTCCTGACCCAGTGCGCTTTACCTTGCCAATGGTTGAAAAAGCCACTACAACTACACCTGTTGTACAAATTGAATTACCAGCACCACCAACTGTAACTGTAGTAGACCAAACATCACCACCAACTGCTGCTGTCACCGTTTCTACATCACAGCCAGTCATAGAAGAGCAGACCACTGTATTTAATCAAACTACTCAGCTAGAACAGCTAAGTGTTAGCGCACCACTGTTAGACCAATCGGAAGTGGAAACGGAAATGGTAGAAGTGCCGTTTGTTGCACCGTCAACAACTACCACTCAACCTCAAGTGGTTACTGTACAAGCACAACCAGCATCAAGTTCGATTCAGTTTCAAGAACCAATTATTAAGGTTGAGTTTGTTAATGAAAGTTTTGATTTCAAAAAACCAAGCCAAACAGCAGCTGCAGCTAGTCAAGCCCCTTCTCAAGCAATTAACATTGCTTTAAATGAAGCTGACCTCATTGATGAATTAGTGGCGGTTGGCACCACAGCCACCACAGCCTTACCACAAAGCGAGCTGATTCAAGAGGTGGTGGTGATTGACAACGGTCAACCCCAACAAGCTGGGTTTCATTATGTAGTTGATTTTCTCACTAGCACTGCACCACTAACTGTAGCAGAAATAGAGTTGCAAGAGCAAGAGCTAGTCAATGAGTTTGTCACAACTACTAGTAGAGAAACAACTACTTTTGCATCAACTCCAGTTTTTGAACCAGTTGTAATTCCAACAGTAGAACCTGAAGAACAACTTTTAGAAAACGAGTTTGTTGAATCAACTGTTGTAAGTGCAACATCAAATGAACCTAATGTTGCATCAACTCCAGTTGTTGAAACGGTTGAACTTACAGAAACACCAGTAAGTTTAGAACCGCTTGAAACTGTTCAACTAGAAACAGCACCAGTTGTTACTGAAACTGTTACAGTTACTGAGAAAGCAGTAGAACCAGAAGTATTAGCAGTTGTTGAAGAAGCACCACTTGCTGTAGAACCAATTGTTGAAACTTCAACAACATTAGCTGCAGAGACCGTTGAAGAAGCGCAAGTTGAACAAGAGTCAACAGCTGTTGCAGTCGAACCAGCTATTGAAACCGAGTCAAAAGCAACTTCTGAAGCCCAAGCTGAACTGGATTGGGAGGCGTTAATTGGTAACAGTGAATACGGTTACTTTGATGCAGAGCAAAACTGGATTTGA", "aa": "MWKGFFDTKLNWIPDPVRFTLPMVEKATTTTPVVQIELPAPPTVTVVDQTSPPTAAVTVSTSQPVIEEQTTVFNQTTQLEQLSVSAPLLDQSEVETEMVEVPFVAPSTTTTQPQVVTVQAQPASSSIQFQEPIIKVEFVNESFDFKKPSQTAAAASQAPSQAINIALNEADLIDELVAVGTTATTALPQSELIQEVVVIDNGQPQQAGFHYVVDFLTSTAPLTVAEIELQEQELVNEFVTTTSRETTTFASTPVFEPVVIPTVEPEEQLLENEFVESTVVSATSNEPNVASTPVVETVELTETPVSLEPLETVQLETAPVVTETVTVTEKAVEPEVLAVVEEAPLAVEPIVETSTTLAAETVEEAQVEQESTAVAVEPAIETESKATSEAQAELDWEALIGNSEYGYFDAEQNWI", "aa_hexdigest": "ac3b7393163d434d5d3ea1605359081a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16713.10", "name": "EAGR_box", "length": 21, "aa_cov": 0.05060240963855422, "hmm_cov": 1.0, "evalue": 2.2396631917305745e-11, "score": 38.74320983886719, "start": 395, "stop": 415 } ], "seq_stats": { "molecular_weight": 44802.23660000011, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_1213", "locus": "GCA000143945_01213" }, { "type": "cds", "sequence": "contig_1", "start": 684613, "stop": 685134, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAAACGATAAAAAAACAGAACCCATCGAACACTACATTACAGTACAAAAAGTATTTAGAACAGTCTAAAGAGAAAACTGCTAAAGCTAAAAATAAGGATGTTAGCATCGATGATTTACTCAAGAAGCCCTTCTTAGAGGAGATCAAAACCAATGTACTTAAGAAGAATAAAACTACCAGAGCTAGCACCGCTACCAGAGGTACTTCTAAAGTCAAAAAACAGATAGTTGAATCTTCTATTGACTTTTTTGACGAGAAAAAGCGTGGTGTTTTTATTGTGCCACCAGCTGGTACTAGTGTTATTAACGATGACCGGGGTGATAATAAAGCGGTAGAGGAGACTGTTAGCAAAACTGCTATTAGTCAAAATCAACTGGCACATTATGCCAACAGTGAGCTTGTTGAAACCGAACAATTTGAACTAAAACCGGTTGCTTTAGAACAGAATCAGGTTCTAACCAGCACCCGCCACAGTCAAGAACGTGAATCGATTTTTGAAAAAGCACAACTCTTTTGA", "aa": "MPKTIKKQNPSNTTLQYKKYLEQSKEKTAKAKNKDVSIDDLLKKPFLEEIKTNVLKKNKTTRASTATRGTSKVKKQIVESSIDFFDEKKRGVFIVPPAGTSVINDDRGDNKAVEETVSKTAISQNQLAHYANSELVETEQFELKPVALEQNQVLTSTRHSQERESIFEKAQLF", "aa_hexdigest": "a28868e9811f6e20fe891d751d4025fa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19616.997699999996, "isoelectric_point": 9.43464183807373 }, "id": "CNOCLNGIAL_1214", "locus": "GCA000143945_01214" }, { "type": "cds", "sequence": "contig_1", "start": 685234, "stop": 685614, "strand": "-", "frame": 3, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "EC:3.6.5.-", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_P47627", "UniRef:UniRef90_P75210" ], "nt": "GTGAAACTACAAGCAAATGAGTTTCGTAAAGATGTTGAATTTTTAGATGCTGACACTGATAACTTTAAGATTTTAGAAGCGCTGCGCGAACAGATCATTAAGTACTGCAGCGAAGAAATTCCCCATGTAGTAAGGCTAGAGATTGTCGATAAGAGTTTTGATCAAGCTAAAAACCTGTTGAAGCTACACCTTTCCATTAGTGTGCCTAAACTTAGTCAAAAAAAAATTATTATTGGCCGTAATGCCCAAATGGTGAAAAAGATCGGGATGCAAATGCGTCAAAAACTACTGGAGTACTATGACTGCAATGTCTTTGTGGAATTGTTTGTAAGGACTTATGACCCTAAACAAAAAAAGTCACCTTATGGCTCTTTAATTTAG", "aa": "MKLQANEFRKDVEFLDADTDNFKILEALREQIIKYCSEEIPHVVRLEIVDKSFDQAKNLLKLHLSISVPKLSQKKIIIGRNAQMVKKIGMQMRQKLLEYYDCNVFVELFVRTYDPKQKKSPYGSLI", "aa_hexdigest": "f967823dbf0c4dafbbd23959c56a2e33", "start_type": "GTG", "rbs_motif": null, "genes": [ "era" ], "truncated": "3-prime", "pseudogene": { "start": 685234, "stop": 685914, "observations": { "mutation": [ 685632 ], "stop": [ 685632 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75210", "query_cov": 1.0, "subject_cov": 0.4329896907216495, "identity": 0.992, "score": 246.0, "evalue": 3.04e-80, "gene_start": 1, "gene_end": 126, "reference_start": 166, "reference_end": 291, "reference_sequence": "MESIRIGVLGLTNAGKSTLVNQLHKANNLLVSPMNNTTLLAVSTNTITHEKQNITFIDVPGFSEKRHSSYELISQEIRKALSGIDVLLLVVRSDQQQKLPLLQTQLQPLKRYRDLTRVLLINNFFDVVLQAEDKQAIVLDFKPQAVLETDLLHFDATSFWNQFNEVKLQANEFRKDVEFLDADTDNFKILEALREQIIKYCSEEIPHVVRLEIVDKSFDQAKNLLKLHLSISVPKLSQKKIIIGRNAQMVKKIGMQMRQKLLEYYDCNVFVELFVRTYDPKQKKSPYGSLI" }, "paralog": false, "identity": 0.995575221238938, "score": 436.0, "evalue": 2.33e-157, "description": "Internal stop codon at 685632. Nonsense mutation around 685632.", "psc": { "uniref90_id": "UniRef90_P75210", "gene": "era", "product": "GTPase Era", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P47627", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75210", "EC:3.6.5.-", "UniRef:UniRef50_P47627", "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47627", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47627" ], "product": "GTPase Era" } }, "id": "CNOCLNGIAL_1215", "locus": "GCA000143945_01215" }, { "type": "cds", "sequence": "contig_1", "start": 685630, "stop": 686109, "strand": "-", "frame": 3, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "EC:3.6.5.-", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_P47627", "UniRef:UniRef90_P75210" ], "nt": "ATGGAATCGATAAGAATTGGTGTTTTAGGTCTAACCAACGCTGGTAAATCAACTCTCGTTAACCAATTACACAAAGCGAATAACTTGTTGGTTTCACCTATGAACAACACTACGTTGTTGGCGGTTAGCACTAATACTATTACCCATGAAAAGCAAAACATTACCTTCATTGATGTTCCTGGCTTTAGTGAAAAACGTCACAGCTCCTATGAGTTAATTAGTCAAGAGATTCGCAAGGCTTTAAGTGGGATAGATGTCTTGTTGTTAGTGGTGCGCAGTGATCAACAGCAAAAGTTACCCTTGTTACAAACGCAATTACAACCGTTAAAGCGTTACCGTGATTTAACTCGTGTTTTGTTAATTAATAACTTCTTTGATGTTGTTTTGCAAGCGGAGGACAAGCAAGCAATTGTGCTCGATTTTAAACCGCAAGCAGTACTGGAAACTGATCTGCTTCATTTTGATGCCACTTCTTTTTGA", "aa": "MESIRIGVLGLTNAGKSTLVNQLHKANNLLVSPMNNTTLLAVSTNTITHEKQNITFIDVPGFSEKRHSSYELISQEIRKALSGIDVLLLVVRSDQQQKLPLLQTQLQPLKRYRDLTRVLLINNFFDVVLQAEDKQAIVLDFKPQAVLETDLLHFDATSF", "aa_hexdigest": "dfe2d35fec5a8946cc6e9fd10130e6a3", "start_type": "ATG", "rbs_motif": null, "genes": [ "era" ], "truncated": "5-prime", "pseudogene": { "start": 685327, "stop": 686109, "observations": { "mutation": [ 685632 ], "stop": [ 685632 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75210", "query_cov": 1.0, "subject_cov": 0.5463917525773195, "identity": 1.0, "score": 297.0, "evalue": 7.31e-100, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MESIRIGVLGLTNAGKSTLVNQLHKANNLLVSPMNNTTLLAVSTNTITHEKQNITFIDVPGFSEKRHSSYELISQEIRKALSGIDVLLLVVRSDQQQKLPLLQTQLQPLKRYRDLTRVLLINNFFDVVLQAEDKQAIVLDFKPQAVLETDLLHFDATSFWNQFNEVKLQANEFRKDVEFLDADTDNFKILEALREQIIKYCSEEIPHVVRLEIVDKSFDQAKNLLKLHLSISVPKLSQKKIIIGRNAQMVKKIGMQMRQKLLEYYDCNVFVELFVRTYDPKQKKSPYGSLI" }, "paralog": false, "identity": 0.9961538461538462, "score": 496.0, "evalue": 1.07e-180, "description": "Internal stop codon at 685632. Nonsense mutation around 685632.", "psc": { "uniref90_id": "UniRef90_P75210", "gene": "era", "product": "GTPase Era", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P47627", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75210", "EC:3.6.5.-", "UniRef:UniRef50_P47627", "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47627", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47627" ], "product": "GTPase Era" } }, "id": "CNOCLNGIAL_1216", "locus": "GCA000143945_01216" }, { "type": "cds", "sequence": "contig_1", "start": 686097, "stop": 686402, "strand": "-", "frame": 1, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "EC:3.1.-.-", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47628", "UniRef:UniRef90_P75209" ], "nt": "ATGAAGCGCTTAAACCGGCAGTTTCTCAACCGCAAAGGGGTAACTGATGTGATTAGCATCTGTTACAACGAAAATGAGGCGGGGTTTTCACCAGCCATTGGTGAAATCTTTTTGTGTCCCAAACACATCTTTAAGCAGGCCAAGCAGTTTGGTTGCACACCTTGGTTTTTACTAACACGCAACTTAATCCATGGTTTGCTACACCTGTTTGAGTTTGACCATGAGCAAAGTCTGGCCTTTGAAAGTGTAACGATGTTTTTTCAAGATGAAATTCATGAAACCGTTTTAAAACTATGGAATCGATAA", "aa": "MKRLNRQFLNRKGVTDVISICYNENEAGFSPAIGEIFLCPKHIFKQAKQFGCTPWFLLTRNLIHGLLHLFEFDHEQSLAFESVTMFFQDEIHETVLKLWNR", "aa_hexdigest": "81f26937fc4e70cc3ffaf4e7419feb3e", "start_type": "ATG", "rbs_motif": null, "genes": [ "ybeY" ], "truncated": "3-prime", "pseudogene": { "start": 686097, "stop": 686561, "observations": { "mutation": [ 686408 ], "stop": [ 686408 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75209", "query_cov": 1.0, "subject_cov": 0.6558441558441559, "identity": 1.0, "score": 213.0, "evalue": 1.08e-69, "gene_start": 1, "gene_end": 101, "reference_start": 54, "reference_end": 154, "reference_sequence": "MKPSFSINSNYLFKRFFGKNFEAGIELCLQIIKDSLQLSFQPEFALMIVSPWKMKRLNRQFLNRKGVTDVISICYNENEAGFSPAIGEIFLCPKHIFKQAKQFGCTPWFLLTRNLIHGLLHLFEFDHEQSLAFESVTMFFQDEIHETVLKLWNR" }, "paralog": false, "identity": 0.987012987012987, "score": 314.0, "evalue": 1.19e-111, "description": "Internal stop codon at 686408. Nonsense mutation around 686408.", "psc": { "uniref90_id": "UniRef90_P75209", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47628", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75209", "EC:3.1.-.-", "UniRef:UniRef50_P47628", "COG:COG0319", "COG:J", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47628", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47628" ], "product": "Endoribonuclease YbeY" } }, "id": "CNOCLNGIAL_1217", "locus": "GCA000143945_01217" }, { "type": "cds", "sequence": "contig_1", "start": 686749, "stop": 686934, "strand": "-", "frame": 3, "gene": null, "product": "putative MG389-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47629", "UniRef:UniRef90_P75208" ], "nt": "ATGAAGCAAAAAATTATTGGCCTAACTCTAGCTTTCTTTGTTTTATTCTTAACAGCCGTTGCCATCTTATTTACGGTTAAGGTACAACGATACCTAACTACAAGCTTATGGGCAAAATTGTCTGAACAAACCTTTTTAGTTTTTAAAAACGAGCAGGATGAAGCCCAGTTTAACCAGGTATCTTGA", "aa": "MKQKIIGLTLAFFVLFLTAVAILFTVKVQRYLTTSLWAKLSEQTFLVFKNEQDEAQFNQVS", "aa_hexdigest": "fbf65018700e9ab47c995fe43f42d65b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 686545, "stop": 686934, "observations": { "mutation": [ 686751 ], "stop": [ 686751 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75208", "query_cov": 1.0, "subject_cov": 0.4728682170542636, "identity": 1.0, "score": 112.0, "evalue": 1.06e-30, "gene_start": 1, "gene_end": 61, "reference_start": 1, "reference_end": 61, "reference_sequence": "MKQKIIGLTLAFFVLFLTAVAILFTVKVQRYLTTSLWAKLSEQTFLVFKNEQDEAQFNQVSWTNFQAETTKKEDKKAFRLYKKKISLEQLENENQQQLFQAVNLSINLKQGWYNITILLPSKALFETVF" }, "paralog": false, "identity": 0.9922480620155039, "score": 248.0, "evalue": 1.66e-86, "description": "Internal stop codon at 686751. Nonsense mutation around 686751.", "psc": { "uniref90_id": "UniRef90_P75208", "product": "Uncharacterized protein MG389 homolog", "uniref50_id": "UniRef50_P47629", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75208", "UniRef:UniRef50_P47629", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47629", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47629" ], "product": "Uncharacterized protein MG389" } }, "id": "CNOCLNGIAL_1218", "locus": "GCA000143945_01218" }, { "type": "cds", "sequence": "contig_1", "start": 686936, "stop": 687244, "strand": "-", "frame": 2, "gene": null, "product": "ABC-type enterochelin transport system, ATPase component", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002E79EA9", "UniRef:UniRef100_A0AAV5N885", "UniRef:UniRef50_A0AAV5N885", "UniRef:UniRef90_A0AAV5N885" ], "nt": "TTGTTAAACTGTAAAAATCCCCAAGTATACCAGCTGTTAGCCGACACCGGCATCCACAACCAGATTAAGTTAAGTTCAGGACAAAAACAGATCTTGCAACTGTTCTTATTACAAAACCTCAAAGACAAGGTGATTTTGTTAGATGAAACACTCAACGCCATTGCCACGGAGCTCAAACCGCGCGTGTATCAGCTTTTGATCAAACCGTTGACCTATAACAACTTTGTTCTGATGGTCGAACACGATTTACGCTTTGTAAACAGTGAGCAAGACCTAATTAACCTCAGTCCTTATTTACAGCAGACATAA", "aa": "MLNCKNPQVYQLLADTGIHNQIKLSSGQKQILQLFLLQNLKDKVILLDETLNAIATELKPRVYQLLIKPLTYNNFVLMVEHDLRFVNSEQDLINLSPYLQQT", "aa_hexdigest": "d1a60becec6b7c5bc540933862ca058b", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002E79EA9", "uniref100_id": "UniRef100_A0AAV5N885", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002E79EA9", "UniRef:UniRef100_A0AAV5N885" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N885", "uniref90_id": "UniRef90_A0AAV5N885" }, "psc": { "uniref90_id": "UniRef90_A0AAV5N885", "product": "ABC-type enterochelin transport system, ATPase component", "uniref50_id": "UniRef50_A0AAV5N885" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N885", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N885" ], "product": "ABC-type enterochelin transport system, ATPase component" }, "genes": [], "id": "CNOCLNGIAL_1219", "locus": "GCA000143945_01219" }, { "type": "cds", "sequence": "contig_1", "start": 687238, "stop": 688230, "strand": "-", "frame": 3, "gene": "sunT", "product": "Putative ABC transporter ATP-binding MG390-like protein", "db_xrefs": [ "COG:COG2274", "COG:V", "GO:0005524", "GO:0006508", "GO:0008234", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P75207", "UniRef:UniRef90_P75207" ], "nt": "ATGCAAACATTACAAACCAAGAGTTTTGTCTACTTTAGCAATAAATCACCTAACCAATTAACCGAGCGTCAGTTTTACCTCAAGGAAGTGTTGTCCTTCTTTAATGTCCATATTCCTAACTTAATTATTAGCTGTACAGTAGCTTTAATTATTGGCACCTTAATTGGTATTAACCAAATGGAGTTCTTATGGATAGCAATTGCCCAAATTGTGGTGAATTGTGCCATTTTTCTTTATGACTTCTTTTTCACTAAACGGATTACGAAGCAAGCAATTCCGCAAATGGAGTTACAAAACAAGGTCAGTTTGCAGTTAGATGGCAATTTGCGTGATGAACAAAACGGCAAGCGCTTCAATTACTTAATGATGCAGTTGCGCAAAGCACTTATTAAAAACCAAAACATTAGTAATCAAAAGGAAGTGAACCACTTAGCGAGTGATGGTGTTAAAAGTTTTGCCCAACAGGTATTTGACTTTTTAATTTTGGCCTTGGGCATTATTGGCATTATTGAACAGCGCTACACCTTAGCCTTTCTCTTTTATATCTTTTCCATTCAGGCATTGTTTTCCGCTTATGCCACGAGAATCATCCAGTTTGGTGCAGCAGTTAACCTGTACCAGTTCTGTAAGGACAAACTAGTTACCTTGTTTGAAGACAAGGTTAATGATTGCAATTTCAAGGTCAGTTGGAAGTGTCCCAAAGTGATTAACTTAAACAACTGTAGTATCACTCTGAACCAAAATCTCGATTTGGCTAACCTCAACCTGAATTTAACTAATGGCATGGTTATTAGTGGTGAGAATGGTAGCGGTAAAAGTACCTTGCTGAAAATTCTGACTGGTCGAGGTTTGTCATACCAGGGTCAAATTAAGTTGGATGAATTAGACCTAAAGGATTTTAGTGCAAGCCAACTGTTTCACAACGTTTACTATTTAACGGGGCAGTTAACGGCCTACAACGACATTACTGACTTTACAGTGAAGCCTTGTTAA", "aa": "MQTLQTKSFVYFSNKSPNQLTERQFYLKEVLSFFNVHIPNLIISCTVALIIGTLIGINQMEFLWIAIAQIVVNCAIFLYDFFFTKRITKQAIPQMELQNKVSLQLDGNLRDEQNGKRFNYLMMQLRKALIKNQNISNQKEVNHLASDGVKSFAQQVFDFLILALGIIGIIEQRYTLAFLFYIFSIQALFSAYATRIIQFGAAVNLYQFCKDKLVTLFEDKVNDCNFKVSWKCPKVINLNNCSITLNQNLDLANLNLNLTNGMVISGENGSGKSTLLKILTGRGLSYQGQIKLDELDLKDFSASQLFHNVYYLTGQLTAYNDITDFTVKPC", "aa_hexdigest": "2cc1c18ca920706a12ef1485b2151151", "start_type": "ATG", "rbs_motif": null, "genes": [ "sunT" ], "truncated": "both", "pseudogene": { "start": 686936, "stop": 688530, "observations": { "deletion": [ 687255 ], "mutation": [ 688236, 688263 ], "stop": [ 688236, 688263 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75207", "query_cov": 0.9848484848484849, "subject_cov": 0.49242424242424243, "identity": 0.997, "score": 621.0, "evalue": 3.32e-219, "gene_start": 1, "gene_end": 325, "reference_start": 229, "reference_end": 553, "reference_sequence": "MKIIYQEQPNECGICVLGMLANELHEDKYAHDELLEQINLPASGLSFFELETYGKKFGLEIASYQLTLEELKQLEGKYFIVHFPKHFVVVHKKQDNLWEVFDPAKGKYLLNDEELKKQWTGYAATVQKSFKEIPPINKRNFFKHFFDLNLIIFYVFIELIIIGISTLLATASKTMIANTVDFGTSVNIVVFVVFFLVLKGLYLLLYALLQMVRNVLFWKQYRGYLGWIMQTLQTKSFVYFSNKSPNQLTERQFYLKEVLSFFNVHIPNLIISCTVALIIGTLIGINQMEFLWIAIVQIVVNCAIFLYDFFFTKRITKQAIPQMELQNKVSLQLDGNLRDEQNGKRFNYLMMQLRKALIKNQNISNQKEVNHLASDGVKSFAQQVFDFLILALGIIGIIEQRYTLAFLFYIFSIQALFSAYATRIIQFGAAVNLYQFCKDKLVTLFEDKVNDCNFKVSWKCPKVINLNNCSITLNQNLDLANLNLNLTNGMVISGENGSGKSTLLKILTGRGLSYQGQIKLDELDLKDFSASQLFHNVYYLTGQLTAYNDITDFGYSEALLNCKNPQVYQLLADTGIHNQIKLSSGQKQILQLFLLQNLKDKVILLDETLNAIATELKPRVYQLLIKPLTYNNFVLMVEHDLRFVNSEQDLINLSPYLQQT" }, "paralog": false, "identity": 0.9924812030075187, "score": 1021.0, "evalue": 0.0, "description": "Internal stop codon at 688236, 688263. Frameshift due to deletion around 687255. Nonsense mutation around 688236, 688263.", "psc": { "uniref90_id": "UniRef90_P75207", "gene": "SunT", "product": "Putative ABC transporter ATP-binding MG390 homolog", "uniref50_id": "UniRef50_P75207", "cog_id": "COG2274", "cog_category": "V", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0008234", "GO:0006508" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75207", "UniRef:UniRef50_P75207", "COG:COG2274", "COG:V", "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0008234", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P75207", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75207" ], "product": "Putative ABC transporter ATP-binding MG390 homolog" } }, "id": "CNOCLNGIAL_1220", "locus": "GCA000143945_01220" }, { "type": "cds", "sequence": "contig_1", "start": 688261, "stop": 688539, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAAATCATTTAAAGAAATTCCGCCCATTAATAAGCGCAATTTCTTTAAGCACTTCTTTGATTTAAACCTGATTATTTTCTATGTCTTCATTGAACTAATCATTATTGGCATTTCAACACTTTTAGCAACGGCCTCTAAAACGATGATTGCCAACACCGTTGACTTTGGGACATCAGTTAACATTGTCGTTTTTGTGGTTTTCTTTTTAGTTTTAAAAGGCTTGTACTTACTTTTGTATGCCTTGTTGCAAATGGTGCGTAATGTTTTGTTTTGA", "aa": "MQKSFKEIPPINKRNFFKHFFDLNLIIFYVFIELIIIGISTLLATASKTMIANTVDFGTSVNIVVFVVFFLVLKGLYLLLYALLQMVRNVLF", "aa_hexdigest": "7666c791d5f5341b6690e0381e40269d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10638.804399999994, "isoelectric_point": 9.69728603363037 }, "id": "CNOCLNGIAL_1221", "locus": "GCA000143945_01221" }, { "type": "cds", "sequence": "contig_1", "start": 688558, "stop": 688914, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTATTTATCAAGAACAACCCAACGAATGTGGCATTTGTGTGCTTGGCATGTTAGCCAATGAACTGCATGAGGATAAGTATGCCCATGATGAACTGTTGGAACAAATTAACCTACCAGCTAGTGGTTTGAGTTTCTTTGAACTAGAGACCTATGGCAAGAAGTTTGGTCTCGAAATTGCTAGTTACCAGCTAACCCTAGAAGAGCTCAAACAACTAGAGGGTAAATACTTTATTGTCCACTTCCCTAAGCATTTTGTGGTGGTGCATAAAAAACAAGATAATCTCTGGGAAGTATTTGACCCCGCTAAAGGGAAATATCTTTTAAATGATGAAGAATTAAAGAAACAGTGA", "aa": "MKIIYQEQPNECGICVLGMLANELHEDKYAHDELLEQINLPASGLSFFELETYGKKFGLEIASYQLTLEELKQLEGKYFIVHFPKHFVVVHKKQDNLWEVFDPAKGKYLLNDEELKKQ", "aa_hexdigest": "ce8098bad00b7f2b0d6cabca416d3d6c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13817.719499999987, "isoelectric_point": 5.183454704284666 }, "id": "CNOCLNGIAL_1222", "locus": "GCA000143945_01222" }, { "type": "cds", "sequence": "contig_1", "start": 689018, "stop": 689248, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCACCCGGATTACCTAAAACCATTGGAATCAAAACTAGCGGACTTACAAAACTCCACTAGTGTTAAGGGAGCAGGTTCATCCCGGGCGGCATGTTTCTTAGCTGAATTTAGGGAAGGGGTACCTTTGATCCACTGTGATATTGCATCCACAGCTTCGATTCAAGATCTTGGTCAAGGGGTTTTAGTACGTACTTTATACGAACGTGCTGCGCAGCAAGCAAAGGAATAA", "aa": "MHPDYLKPLESKLADLQNSTSVKGAGSSRAACFLAEFREGVPLIHCDIASTASIQDLGQGVLVRTLYERAAQQAKE", "aa_hexdigest": "4fbf813e5ddb1cf735b4bca6149ef76a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 73, "aa_cov": 0.8552631578947368, "hmm_cov": 0.9863013698630136, "evalue": 4.040391320163549e-12, "score": 41.44631576538086, "start": 2, "stop": 66 } ], "seq_stats": { "molecular_weight": 8203.218999999997, "isoelectric_point": 6.064061164855956 }, "id": "CNOCLNGIAL_1223", "locus": "GCA000143945_01223" }, { "type": "cds", "sequence": "contig_1", "start": 689258, "stop": 690355, "strand": "-", "frame": 2, "gene": "pepA", "product": "leucyl aminopeptidase", "db_xrefs": [ "COG:COG0260", "COG:E", "EC:3.4.11.1", "GO:0005737", "GO:0006508", "GO:0030145", "GO:0070006", "SO:0001217", "UniRef:UniRef50_P47631", "UniRef:UniRef90_P75206" ], "nt": "ATGAAATTAAATAAGTTATTTGACAGCAAGACGGTTGTTTTTAAAAAGTCCGATAAGTACGGCAAGAGTGATTGCTGTAAGACTAAGTGTGTTGAAGGCCAAATTGAATTTGGCGTAAAAATTCCGACTGATTTCCCCGCTTTCAACCGCGCTCTAGTTACCTTTTTGAAAACGCAAAAGAACCAACTAAACGTGGACTTAGACAGTTTTGTGGAACTCTACAAAGCAGAAAACCTCTGTTACAAAACAGCATTAAAGGTAGTGGTAGCTAGCATTACCTTCTGTGAAACAACACCTTTCACCATGAAGACCGAACCACAAAAGAATGTGGAAGTAGCAGTGAAGTGTGACAGCGAACACACGAGCCTCATTAAAGAATACGAGGTAGTGGGTAACTATGTCAACATGGCGCGTCAACTCCAAGACACCCCCTCTGACCAACTCTACCCAGAGGAATTTGTGAAGCGCTTTGAAAAGGCGGCTACTGGTTTAGGTGTTAAGATTACCGTTTTAAAGCAAGCTGATCTCATCAAAAAGAAGATGGGCTTACTGTTAGGGGTTAACAAGGGTTCGGAACGTGAAGCGCGTTTACTAGTAATTTCCTATAACAATAATAAGAAGAGCAGTGAAACTTTAGCTTTAGTTGGTAAGGGCATTACCTATGATTCGGGTGGGATGAACATTAAGACCGGTGACTACATGCGTGGTATGAAGTACGACATGAGTGGGGCAGCTATTGTGTGTTCCACCGTGTTGGCCTTAGCGAAAAACAAGGTGAAAACCAATGTAGTAGCAGTAGCTGCTTTAACAGAAAACCTACCAGGTCCCCACGCACAACGCCCTGATGACATCCAAACCGCTTACAATGGTAAAACGGTTGAAATTGACAATACCGATGCTGAAGGTCGCTTGGTGTTAGCCGATGCCATTAGCTATGCTGCTAAGGATCTTAAAGCTACCCAAATTATTGATGTCGCTACCTTAACCGGTTTAATGTCTTACATCTTAAGTACCACTTACACCGGGATCTTTAGTACCTGTGACATGGCATGGGATGCCTTTAAAAAAGCAGCTTGCTGTGCAGGCGAACCGGTATGA", "aa": "MKLNKLFDSKTVVFKKSDKYGKSDCCKTKCVEGQIEFGVKIPTDFPAFNRALVTFLKTQKNQLNVDLDSFVELYKAENLCYKTALKVVVASITFCETTPFTMKTEPQKNVEVAVKCDSEHTSLIKEYEVVGNYVNMARQLQDTPSDQLYPEEFVKRFEKAATGLGVKITVLKQADLIKKKMGLLLGVNKGSEREARLLVISYNNNKKSSETLALVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGPHAQRPDDIQTAYNGKTVEIDNTDAEGRLVLADAISYAAKDLKATQIIDVATLTGLMSYILSTTYTGIFSTCDMAWDAFKKAACCAGEPV", "aa_hexdigest": "fdd1ae83e24b3cd833bd1a60de804876", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75206", "query_cov": 1.0, "subject_cov": 0.8202247191011236, "identity": 1.0, "score": 709.0, "evalue": 9.43e-257, "valid": true, "gene": "pepA", "product": "leucyl aminopeptidase", "ec_ids": [ "3.4.11.1" ], "uniref50_id": "UniRef50_P47631", "cog_id": "COG0260", "cog_category": "E", "go_ids": [ "GO:0005737", "GO:0030145", "GO:0070006", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47631", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47631" ], "product": "Probable cytosol aminopeptidase" }, "genes": [ "pepA" ], "id": "CNOCLNGIAL_1224", "locus": "GCA000143945_01224" }, { "type": "cds", "sequence": "contig_1", "start": 690369, "stop": 692000, "strand": "-", "frame": 1, "gene": "groL", "product": "chaperonin GroEL", "db_xrefs": [ "COG:COG0459", "COG:O", "EC:5.6.1.7", "GO:0005524", "GO:0005737", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0140662", "RefSeq:WP_010874930.1", "SO:0001217", "UniParc:UPI00001276EF", "UniRef:UniRef100_P78012", "UniRef:UniRef50_P47632", "UniRef:UniRef90_P78012" ], "nt": "ATGGCAAAGGAATTAGTATTTGGCAAGAATGCCCGCAATAAACTGCTAGCTGGTATTAATAAATTAGCAGACGCTGTTAAGGTAACCGTTGGTCCTAAAGGTCAAAACGTGATTTTAGGTCGGAAGTTTTCCAATCCTTTAATTACTAACGATGGAGTGACCATCGCTAAGGAAATTGAACTAACTGATCCTTTAGAAAACATTGGTGCTAAGGTTATTTCGGTAGCTGCTGTATCTACCAATGATATTGCTGGGGACGGTACTACCACTGCTACCATCTTGGCACAGGAAATGACTAACCGTGGTGTGGAAGCTGTTAATAATGGCGCTAATCCAGTTAATGTCCGCCGTGGGATTGAAGATGCTAGTCAGTTAATTATTACTGAGTTAGATAAGCGTTCCAAAAAGATTAATACCAACGAAGAAATCGAGCAAGTTGCAGCGATCTCTTCCGGTTCCAAGGAAATTGGGAAGCTGATTGCTCAAGCTATGGCCTTAGTTGGTAAAAACGGTGTCATTACCACCGATGATGCTAAGACGATTAACACTACCCTAGAAACCACTGAAGGGATTGAGTTTAAAGGTACTTACGCTTCCCCTTACATGGTTAGCGATCAAGAAAAAATGGAAGTGGTGTTAGACCAACCGAAGATCTTGGTCAGTGCGATGAAGATTAACACCATTAAGGAAATCTTACCGTTACTAGAAGGTAGCATGGAAAACGGTAACCCACTGTTAATCGTGGCACCTGACTTTGCTGAAGAAGTGGTAACTACTTTAGCGGTGAACAAGCTTCGCGGTACCATTAATGTAGTTGCTGTTAAATGTAACGAATACGGGGAACGCCAAAAGGCAGCTTTGGAAGATTTAGCCATTAGTACTGGCACCTTAGCTTACAACAATGAACTAGGTGGTGGCTTTAAGGATGTGACTGTAAACCACTTAGGGGAAGCTCGCCGTGTGCAAGTAGCCAAAGAAAAAACCACGGTTATTGGTGGTAAGGGTTCTAAGGAAACGATCCAAAAACACCTTGACCTTCTAAATGGTCGTTTAAAGCAAACTACCGAAAAGTACGATACCGATCTGTTAAAGGAACGGATTGCCCACCTCAGTCAAGGGGTAGCTGTAGTACGGGTTGGTGGTGCTACGGAACTAGCACAAAAAGAGTTAAAACTCCGCATTGAAGATGCCTTAAACTCGACTAAGGCAGCTGTTGAAGAAGGGATTATTTCCGGGGGTGGTATTGCTTTACTTAACGTATCCACTATCCTCAATGACAGTAAGTTGGCTGACAAGTACAAGGCAGAAACTAGTGCGGAAAACCTCAAGGAAATCCTGGTTGGTTATGAAATTGTGCGTAAGTCACTAGAAGCACCAGTGCGCCAAATTATTGAAAACTCCGGGGTTAACCCAGTTAAAGTATTTGCAGAACTACGCAGTGAAGCTGATGGTGTTGGTTTTGATGCTGAAACCAAAAAGAAGGTCGACATGATCCGCAGCGGTATTATTGACCCTACTAAGGTAACCAAAACTGCACTCGAAAAAGCTGCATCAGTAGCAAGTTCCTTAATTACCACCAGCGTTGCTGTGTATGATATTAAGGAAAACAAGGAAGGTAGCTTCCAAGAATAG", "aa": "MAKELVFGKNARNKLLAGINKLADAVKVTVGPKGQNVILGRKFSNPLITNDGVTIAKEIELTDPLENIGAKVISVAAVSTNDIAGDGTTTATILAQEMTNRGVEAVNNGANPVNVRRGIEDASQLIITELDKRSKKINTNEEIEQVAAISSGSKEIGKLIAQAMALVGKNGVITTDDAKTINTTLETTEGIEFKGTYASPYMVSDQEKMEVVLDQPKILVSAMKINTIKEILPLLEGSMENGNPLLIVAPDFAEEVVTTLAVNKLRGTINVVAVKCNEYGERQKAALEDLAISTGTLAYNNELGGGFKDVTVNHLGEARRVQVAKEKTTVIGGKGSKETIQKHLDLLNGRLKQTTEKYDTDLLKERIAHLSQGVAVVRVGGATELAQKELKLRIEDALNSTKAAVEEGIISGGGIALLNVSTILNDSKLADKYKAETSAENLKEILVGYEIVRKSLEAPVRQIIENSGVNPVKVFAELRSEADGVGFDAETKKKVDMIRSGIIDPTKVTKTALEKAASVASSLITTSVAVYDIKENKEGSFQE", "aa_hexdigest": "c64a2abdaf698cb2342265bde9053f1a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001276EF", "ncbi_nrp_id": "WP_010874930.1", "uniref100_id": "UniRef100_P78012", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001276EF", "RefSeq:WP_010874930.1", "UniRef:UniRef100_P78012" ] }, "ips": { "uniref100_id": "UniRef100_P78012", "uniref90_id": "UniRef90_P78012" }, "psc": { "uniref90_id": "UniRef90_P78012", "gene": "groL", "product": "chaperonin GroEL", "ec_ids": [ "5.6.1.7" ], "uniref50_id": "UniRef50_P47632", "cog_id": "COG0459", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_P47632", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47632" ], "product": "Chaperonin GroEL" }, "genes": [ "groL" ], "id": "CNOCLNGIAL_1225", "locus": "GCA000143945_01225" }, { "type": "cds", "sequence": "contig_1", "start": 692003, "stop": 692353, "strand": "-", "frame": 2, "gene": "groES", "product": "co-chaperone GroES", "db_xrefs": [ "COG:COG0234", "COG:O", "GO:0005524", "GO:0005737", "GO:0044183", "KEGG:K04078", "RefSeq:WP_010874931.1", "SO:0001217", "UniParc:UPI0000127635", "UniRef:UniRef100_P75205", "UniRef:UniRef50_P47633", "UniRef:UniRef90_P75205" ], "nt": "ATGAAGATAACACCAATTCATGACAACATTTTGGTTTCACTAGTGGAATCAAACAAAGAAGAAGTGTCACAAAAAGGGATTATTACAGCACTAGCTAACCCTGATAAAAATGAAAGTGCCCACAAGGGAACTGTCGTTGCTTTAGGTGCAGGACCGGCATATGGTAAAAGCGAAAAACCGAAATATGCTTTTGGCATCGGTGACACGATCTACTTTAAGGAATACAGTGGTATTTCCTTTGAAGAGGAAGGTACCAAGTACAAGATTATTTCCTTGGAAGATGTCCTTGCTTTTGAAAAGCATGGTAATACAAAAACTACTACTGTAAAGAAAGGAGCTAAGAAAAAATAG", "aa": "MKITPIHDNILVSLVESNKEEVSQKGIITALANPDKNESAHKGTVVALGAGPAYGKSEKPKYAFGIGDTIYFKEYSGISFEEEGTKYKIISLEDVLAFEKHGNTKTTTVKKGAKKK", "aa_hexdigest": "ed0cbe8839984c80d949ab01ca30e622", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000127635", "ncbi_nrp_id": "WP_010874931.1", "uniref100_id": "UniRef100_P75205", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000127635", "RefSeq:WP_010874931.1", "UniRef:UniRef100_P75205" ] }, "ips": { "uniref100_id": "UniRef100_P75205", "uniref90_id": "UniRef90_P75205" }, "psc": { "uniref90_id": "UniRef90_P75205", "gene": "groES", "product": "co-chaperone GroES", "uniref50_id": "UniRef50_P47633", "cog_id": "COG0234", "cog_category": "O", "kegg_orthology_id": "K04078", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0044183" ] }, "pscc": { "uniref50_id": "UniRef50_P47633", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47633" ], "product": "Co-chaperonin GroES" }, "genes": [ "groES" ], "id": "CNOCLNGIAL_1226", "locus": "GCA000143945_01226" }, { "type": "cds", "sequence": "contig_1", "start": 692541, "stop": 692927, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_575", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014325607.1", "SO:0001217", "UniParc:UPI0001DD6434", "UniRef:UniRef100_A0AAX0SS40", "UniRef:UniRef50_P75204", "UniRef:UniRef90_P75204" ], "nt": "ATGCTGCAAAATTTAGCTTTAAGCTTTCCTTTTATTACGCGTTTCTTCCAAAAGCAAATGCTTGGTTCCCAGAATTCTAGTGGTAAAACTCCTGGTTTCAATGAAGCCGAAGGAATAACGAGTAACATTTTCCAAATTGCTGGTGGAATTAGCCTTTTAGTTATTTTGTTGTTAATCATTGGTTTCTTGAGTTGTTTGTTGGGTGGCATCTTCCTACATAAGCACAAACACGCTGAAGTGGGTAGTCCCGCTCATGCAAAAACGAAAAATCTATTTGTTGCTTTCTTTGTTGTCGGTAGCTTATTGTTGTTAGTGGCCGTAGTCATGTTAATTGCTTTCGGTGTGCTTGATGCTAGTCTGCCACTTCCAAAAGAAAATAACTCTTAA", "aa": "MLQNLALSFPFITRFFQKQMLGSQNSSGKTPGFNEAEGITSNIFQIAGGISLLVILLLIIGFLSCLLGGIFLHKHKHAEVGSPAHAKTKNLFVAFFVVGSLLLLVAVVMLIAFGVLDASLPLPKENNS", "aa_hexdigest": "d40087e101e7fb6e59f9bb4b6e8b0ef0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6434", "ncbi_nrp_id": "WP_014325607.1", "uniref100_id": "UniRef100_A0AAX0SS40", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6434", "RefSeq:WP_014325607.1", "UniRef:UniRef100_A0AAX0SS40" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SS40", "uniref90_id": "UniRef90_P75204" }, "psc": { "uniref90_id": "UniRef90_P75204", "product": "Uncharacterized protein MPN_575", "uniref50_id": "UniRef50_P75204", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75204", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75204" ], "product": "Uncharacterized protein MPN_575" }, "genes": [], "id": "CNOCLNGIAL_1227", "locus": "GCA000143945_01227" }, { "type": "cds", "sequence": "contig_1", "start": 693361, "stop": 693618, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "TTGCAACAAGCCAACATTGTGTTGAACATGAACACGGTTCCTTTCGATAAGAATCCTGCCATAAATCCTTCCGGTATTCGTATTGGTACACCTGCCATGACTACTCGTGGCTTTAAGGAAAAGCACTTCCTTTATGTTGCTGCCTTAATTGACAAGATTATTAAATCTGACGGTAATAAGAAAGTTATTAAAGAAGTAAAAAAAGCGGTTTTAAAACTGTTAGAACGTTTTCCGTTGTATAAGGGATTGGAGTATTAG", "aa": "MQQANIVLNMNTVPFDKNPAINPSGIRIGTPAMTTRGFKEKHFLYVAALIDKIIKSDGNKKVIKEVKKAVLKLLERFPLYKGLEY", "aa_hexdigest": "1c7ac642cdc9098ecaa540745a491ff2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 49, "aa_cov": 0.5764705882352941, "hmm_cov": 0.9795918367346939, "evalue": 1.5794686905312382e-14, "score": 48.78683853149414, "start": 2, "stop": 50 } ], "seq_stats": { "molecular_weight": 9617.351999999995, "isoelectric_point": 9.939171409606931 }, "id": "CNOCLNGIAL_1228", "locus": "GCA000143945_01228" }, { "type": "cds", "sequence": "contig_1", "start": 693619, "stop": 694335, "strand": "-", "frame": 3, "gene": "glyA", "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "COG:COG0112", "COG:E", "EC:2.1.2.1", "GO:0004372", "GO:0005829", "GO:0019264", "GO:0030170", "GO:0035999", "KEGG:K00600", "SO:0001217", "UniRef:UniRef50_P47634", "UniRef:UniRef90_P78011" ], "nt": "GTGCAACCGCACTCCGGTTCTTCTGCTAACTATGCCGTGTACTTAGCTCTACTTAAACCTGGCGATGCCATTTTAGGGCTTGACCTTAACTGTGGTGGGCACTTAACTCACGGTAATAAATTTAACTTTTCGGGTAAACAGTACCAGCCTTATTCCTACACCATCAACCCCGAAACCGAGATGTTGGACTATGATGAGGTGCTACGTGTTGCTCGGGAGGTAAAACCTAAACTAATTATCTGCGGTTTTTCCAATTACTCGAGAACCGTTGACTTTGAGCGCTTTAGTGCAATTGCGAAAGAAGTTGGTGCTTACCTTTTAGCGGACATTGCCCACATTGCGGGATTGGTAGCAGCTGGCTTGCACCCCAATCCCTTACCTTACGCTGATGTGGTTACATCTACTACCCACAAAACACTGCGCGGTCCACGGGGAGGGTTAATTATGTCCAATAACGAAGCGATTATTCGCAAATTGGACAGCGGTGTCTTTCCGGGTTGTCAAGGTGGTCCCTTACAGCACGTAATTGCGGCTAAGTATGTTTGTTTTAAAGAAGCTTTACAACCAAAATACAAGCAGTACATCCAAAACGTTAAGACCAATGCAGCGAGCATGGCTAGTTGGTTCAAACAACAAGGTTACCGCGTCATTTCTAACGGAACTGATACCCACTTGTTCTCGCTTGATGTCGGTAAGGGTAAAGATGTTTCACAATGA", "aa": "MQPHSGSSANYAVYLALLKPGDAILGLDLNCGGHLTHGNKFNFSGKQYQPYSYTINPETEMLDYDEVLRVAREVKPKLIICGFSNYSRTVDFERFSAIAKEVGAYLLADIAHIAGLVAAGLHPNPLPYADVVTSTTHKTLRGPRGGLIMSNNEAIIRKLDSGVFPGCQGGPLQHVIAAKYVCFKEALQPKYKQYIQNVKTNAASMASWFKQQGYRVISNGTDTHLFSLDVGKGKDVSQ", "aa_hexdigest": "8cc5e5c0d67e5f8f13ea84f29180dfa4", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FEF2304", "query_cov": 0.9705882352941176, "subject_cov": 0.9352226720647774, "identity": 0.628, "score": 286.0, "evalue": 6.81e-95, "valid": false, "product": "serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A661GFR2", "kegg_orthology_id": "K00600" }, "genes": [ "glyA" ], "truncated": "both", "pseudogene": { "start": 693361, "stop": 694581, "observations": { "mutation": [ 693621, 694344 ], "stop": [ 693621, 694344 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78011", "query_cov": 1.0, "subject_cov": 0.5862068965517241, "identity": 0.992, "score": 484.0, "evalue": 2.25e-170, "gene_start": 1, "gene_end": 238, "reference_start": 83, "reference_end": 320, "reference_sequence": "MEPKIRRILNKELQRQRDCICLIASENYVSRDILEVTGSILTNKYAEGYPTRRFYEGCEVVDESESLAINTCKELFGAKWANVQPHSGSSANYAVYLALLKPGDAILGLDLNCGGHLTHGNKFNFSGKQYQPYSYTINPETEMLDYDEVLRVAREVKPKLIICGFSNYSRTVDFERFSAIAKEVGAYLLADIAHIAGLVAAGLHPNPLPYTDVVTSTTHKTLRGPRGGLIMSNNEAIIRKLDSGVFPGCQGGPLQHVIAAKYVCFKEALQPKYKQYIQNVKTNAASMASWFKQQGYRVISNGTDTHLFSLDVGKGKDVSQWLQQANIVLNMNTVPFDKNPAINPSGIRIGTPAMTTRGFKEKHFLYVAALIDKIIKSDGNKKVIKEVKKAVLKLLERFPLYKGLEY" }, "paralog": false, "identity": 0.9926108374384236, "score": 809.0, "evalue": 5.05e-301, "description": "Internal stop codon at 693621, 694344. Nonsense mutation around 693621, 694344.", "psc": { "uniref90_id": "UniRef90_P78011", "gene": "glyA", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P47634", "cog_id": "COG0112", "cog_category": "E", "kegg_orthology_id": "K00600", "go_ids": [ "GO:0005829", "GO:0004372", "GO:0030170", "GO:0019264", "GO:0035999" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78011", "EC:2.1.2.1", "UniRef:UniRef50_P47634", "COG:COG0112", "COG:E", "KEGG:K00600", "GO:0005829", "GO:0004372", "GO:0030170", "GO:0019264", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_P47634", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47634" ], "product": "Serine hydroxymethyltransferase" } }, "id": "CNOCLNGIAL_1229", "locus": "GCA000143945_01229" }, { "type": "cds", "sequence": "contig_1", "start": 694342, "stop": 694581, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "ATGGAACCAAAGATTAGAAGAATCCTCAATAAGGAATTGCAAAGACAAAGAGATTGCATTTGTTTAATAGCATCAGAGAACTATGTGAGTAGGGACATCCTAGAGGTCACTGGTTCCATTTTGACCAATAAGTACGCTGAGGGTTATCCTACTAGACGCTTTTATGAAGGTTGTGAGGTAGTTGATGAATCAGAGAGCTTAGCCATTAATACCTGTAAGGAATTATTTGGTGCGAAGTGA", "aa": "MEPKIRRILNKELQRQRDCICLIASENYVSRDILEVTGSILTNKYAEGYPTRRFYEGCEVVDESESLAINTCKELFGAK", "aa_hexdigest": "99b359e63f0562419aa7e22af2aef923", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1F2Y6L7", "query_cov": 0.9367088607594937, "subject_cov": 0.8505747126436781, "identity": 0.5539999999999999, "score": 83.6, "evalue": 1.76e-19, "valid": false, "product": "Serine hydroxymethyltransferase-like domain-containing protein", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A838MK70", "kegg_orthology_id": "K00600" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 77, "aa_cov": 0.9746835443037974, "hmm_cov": 1.0, "evalue": 1.7842713467869579e-29, "score": 98.00299072265625, "start": 2, "stop": 78 } ], "seq_stats": { "molecular_weight": 9119.335399999994, "isoelectric_point": 5.453383064270019 }, "id": "CNOCLNGIAL_1230", "locus": "GCA000143945_01230" }, { "type": "cds", "sequence": "contig_1", "start": 694659, "stop": 694796, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004F7CA", "UniRef:UniRef100_A0AB38W5S7", "UniRef:UniRef50_A0AB38W5S7", "UniRef:UniRef90_A0AB38W5S7" ], "nt": "ATGAGTGTAGTGTTGTTTGGCAAATTGGGTATAGTAATGGGTAGTATTGCGGTTGCCAAAGATAAGATCATAACCAATGCTGCTCTAGTTCGGATTGAGGGAATTGCCCTCACCCTGAAAGCGCAGCATTTGGACTAA", "aa": "MSVVLFGKLGIVMGSIAVAKDKIITNAALVRIEGIALTLKAQHLD", "aa_hexdigest": "a682c9f0a1623f3cff503bf06613f5af", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001004F7CA", "uniref100_id": "UniRef100_A0AB38W5S7", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004F7CA", "UniRef:UniRef100_A0AB38W5S7" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W5S7", "uniref90_id": "UniRef90_A0AB38W5S7" }, "psc": { "uniref90_id": "UniRef90_A0AB38W5S7", "uniref50_id": "UniRef50_A0AB38W5S7" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5S7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4719.697099999999, "isoelectric_point": 9.52289905548096 }, "id": "CNOCLNGIAL_1231", "locus": "GCA000143945_01231" }, { "type": "cds", "sequence": "contig_1", "start": 694743, "stop": 694946, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGTATGGTCATGGTTTAGGAATCGATCAGGTTAACCTAAGAAAAGGGACTTCTAGTTCTTTAGTGATTAACCAAAAGCGTCAAATTGTGGGAATCTATTTTGCTACGGTAATTACTAATCCCAAGAAGGCTGTTCGTAACGATGTTGGTTTAGTCCAAATGCTGCGCTTTCAGGGTGAGGGCAATTCCCTCAATCCGAACTAG", "aa": "MYGHGLGIDQVNLRKGTSSSLVINQKRQIVGIYFATVITNPKKAVRNDVGLVQMLRFQGEGNSLNPN", "aa_hexdigest": "8973777533218428a702c0160bd05934", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 34, "aa_cov": 0.5074626865671642, "hmm_cov": 1.0, "evalue": 3.3006222484092315e-08, "score": 28.994007110595703, "start": 2, "stop": 35 } ], "seq_stats": { "molecular_weight": 7357.3937000000005, "isoelectric_point": 10.277243614196774 }, "id": "CNOCLNGIAL_1232", "locus": "GCA000143945_01232" }, { "type": "cds", "sequence": "contig_1", "start": 695007, "stop": 695648, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75203", "UniRef:UniRef90_P75203" ], "nt": "TTGAGCAATCCGAAGGACTATGCACCGTTTAATAAAGCTAGCATTGGCAACAGTTTAACTACGGTATTTTGTCTAGGGAAATATATTAACCCTCAATTGTTTAAGCTACGTACTGATGTAAGCAACGCCTTTGTGTCAATACAAACTTCCACTATTCCCAAAACAGCCTTTGTAGCACGCGACTTTGTGCCCTTACAAAACCGCGGTAACCAGTGGGTAGCACCAGTTCGTGCATCAGAAGATGATCCTGCACTAGCAAAAAGCTATTTGGATTTTGCGATCATAGAAGTTCCACTCTTTTTACACAACCAAATGGATAAACAGATCTATGACCACTTTATGCGCCCCGCCATCAATACCTATGAACGGTTAGGTAACAGTGTGGGAATCTTTGCTTACCAACCTATGGCAAGCTTTAAGCGAGATAGCTATTTTGCTTTAGGTTATCCCCAAGTAGAGAGCAATATTGCTGCACTTAACTTGAACCAAACTGAAGTTAAACCTACTCGCCCAGAGGATGTTGCTCAAGTTACTTTTAAAGAGCCTTGGTCAGTAGATCACCACCGCGAAATTCCTACTTTGACTACCAATCAACTTACCACCATTAAAACAAAACACTTTTCTGGTAGTAAGTTATCTTGA", "aa": "MSNPKDYAPFNKASIGNSLTTVFCLGKYINPQLFKLRTDVSNAFVSIQTSTIPKTAFVARDFVPLQNRGNQWVAPVRASEDDPALAKSYLDFAIIEVPLFLHNQMDKQIYDHFMRPAINTYERLGNSVGIFAYQPMASFKRDSYFALGYPQVESNIAALNLNQTEVKPTRPEDVAQVTFKEPWSVDHHREIPTLTTNQLTTIKTKHFSGSKLS", "aa_hexdigest": "0ad465293935d5aa7e54199c4b8c9619", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 694743, "stop": 695903, "observations": { "mutation": [ 695009, 695729, 695741, 695762, 695861 ], "stop": [ 695009, 695729, 695741, 695762, 695861 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75203", "query_cov": 1.0, "subject_cov": 0.5518134715025906, "identity": 0.995, "score": 429.0, "evalue": 2.31e-149, "gene_start": 1, "gene_end": 213, "reference_start": 86, "reference_end": 298, "reference_sequence": "MKTVTAAGGVKRKEWKTNYLINTIPKKSEALHDLYDLSFSMQLANNKWRVFGTGWLIDWKKPKRTHNLSEPFYLYLATNLHIAVALSNPKDYAPFNKASIGNSLTTVFCLGKYINPQLFKLRTDVSNAFVSIQTSTIPKTAFVARDFVPLQNRGNQWVAPVRASEDDPALAKSYLDFAIIEVPLFLHNQMDKQIYDHFMRPAINTYERLGNSVGIFAYQPMASFKRDSYFALGYPQVESNIAALNLNQTEVKPTRPEDVAQVTFKEPWSVDHHREIPTLTTNQLTTIKTKHFSGSKLSWPFDHTKSFKIKNKWLGQNYQMYGHGLGIDQVNLRKGTSSSLVINQKRQIVGIYFATVITNPKKAVRNDVGLVQMLRFQGEGNSLNPN" }, "paralog": false, "identity": 0.9844559585492227, "score": 759.0, "evalue": 2.8e-282, "description": "Internal stop codon at 695009, 695729, 695741, 695762, 695861. Nonsense mutation around 695009, 695729, 695741, 695762, 695861.", "psc": { "uniref90_id": "UniRef90_P75203", "product": "Uncharacterized protein MPN_577", "uniref50_id": "UniRef50_P75203", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75203", "UniRef:UniRef50_P75203" ] }, "pscc": { "uniref50_id": "UniRef50_P75203", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75203" ], "product": "Uncharacterized protein MPN_577" } }, "id": "CNOCLNGIAL_1233", "locus": "GCA000143945_01233" }, { "type": "cds", "sequence": "contig_1", "start": 697010, "stop": 697432, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_580", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "RefSeq:WP_014325610.1", "SO:0001217", "UniParc:UPI0002386D12", "UniRef:UniRef100_A0AAV5N765", "UniRef:UniRef50_P75200", "UniRef:UniRef90_P75200" ], "nt": "ATGACCGCCCAGTATTACCAACTAGACGAAAAGTTTAATCAGCAAACCTATGAACAGTATGGTAAAGGCTTAGCCTTAAATGATTTGTATATCCGCAGGGGCGCTTCTGGTAGTTTAGTCTTCAATCAGGATCAACAAATATCAGGCATCTTTTTTGCAGTAGCTAGTCATTCCGACCCTAAACCTGGTGAAAAAACTCTGGTACAACTGTTACGCTTGCCAGTTGATGAAAGTACTACAGCGACGGTAAAGGACAACTGTGTGCCCTATGATTTAATCTTTGGCAACCGCAATACTACCCATTACTATACCCAATTTGCCAAGCAACACCACACCCATTTGTATGAGCAAATTCAACAAAGTAATGACCAGTTAATTAAGTTTATTGATCGCGAAAATGTGTCCTGTTCAATGGTTTCGTAA", "aa": "MTAQYYQLDEKFNQQTYEQYGKGLALNDLYIRRGASGSLVFNQDQQISGIFFAVASHSDPKPGEKTLVQLLRLPVDESTTATVKDNCVPYDLIFGNRNTTHYYTQFAKQHHTHLYEQIQQSNDQLIKFIDRENVSCSMVS", "aa_hexdigest": "ac553a85c54f196a69ccad62e60b5e83", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386D12", "ncbi_nrp_id": "WP_014325610.1", "uniref100_id": "UniRef100_A0AAV5N765", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386D12", "RefSeq:WP_014325610.1", "UniRef:UniRef100_A0AAV5N765" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N765", "uniref90_id": "UniRef90_P75200" }, "psc": { "uniref90_id": "UniRef90_P75200", "product": "Uncharacterized protein MPN_580", "uniref50_id": "UniRef50_P75200", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P75200", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75200" ], "product": "Uncharacterized protein MPN_580" }, "genes": [], "id": "CNOCLNGIAL_1234", "locus": "GCA000143945_01234" }, { "type": "cds", "sequence": "contig_1", "start": 697515, "stop": 697901, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_581", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75199", "UniRef:UniRef90_P75199" ], "nt": "ATGGCTTATGCAGCGGTGGACTTTGTTGATTATCGGTTTGACAATAAACATTGGAAATCCTTAAATGAAATTTGTCCGGAAGTTGGTTTAAAACCTTATGCTGATTTTGCCGTGTTAGAAGTACCCTTGTACTTAAACAACAAGTTGGATTACCAAGTATGGGAAAACTTTATCAAACCCGCCATTGCTACCTATAAGGCCTTAGGTGATAGTACAAACTTGTTTGCAAGTACGTCATCTTCTGATTTACAAAATGACACCTATTTTGCTCTCGGTTATCCCTTACTAGAGAGTAACATTGATGCGATTCACTTTAACCAAACAGGTGCCACTTTAACTAGTGTTCCGGTTAAAGACCGCATTAAAGTTAAAAACCAAAGCTTCTGA", "aa": "MAYAAVDFVDYRFDNKHWKSLNEICPEVGLKPYADFAVLEVPLYLNNKLDYQVWENFIKPAIATYKALGDSTNLFASTSSSDLQNDTYFALGYPLLESNIDAIHFNQTGATLTSVPVKDRIKVKNQSF", "aa_hexdigest": "7af1985afa574e988a4eafa9b8f1ad0b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 697500, "stop": 698201, "observations": { "mutation": [ 697517, 698135, 698168 ], "stop": [ 697517, 698135, 698168 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75199", "query_cov": 1.0, "subject_cov": 0.4830188679245283, "identity": 1.0, "score": 264.0, "evalue": 1.47e-87, "gene_start": 1, "gene_end": 128, "reference_start": 133, "reference_end": 260, "reference_sequence": "MQLEKVPRAEYQTKWATNTVPKPTDFYHQLYNLSFSLTLTSQRWNTYGTGWLIDWKDTSTNENKFTAYLATNFHVADTLKNPHDYPPYNQVDNTQDLTTSFRIGKYTDPSLFGLYTNVKHAFVDVQLAALPKMAYAAVDFVDYRFDNKHWKSLNEICPEVGLKPYADFAVLEVPLYLNNKLDYQVWENFIKPAIATYKALGDSTNLFASTSSSDLQNDTYFALGYPLLESNIDAIHFNQTGATLTSVPVKDRIKVKNQSFWTLNK" }, "paralog": false, "identity": 0.9871244635193133, "score": 468.0, "evalue": 2.3e-170, "description": "Internal stop codon at 697517, 698135, 698168. Nonsense mutation around 697517, 698135, 698168.", "psc": { "uniref90_id": "UniRef90_P75199", "product": "Uncharacterized protein MPN_581", "uniref50_id": "UniRef50_P75199", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75199", "UniRef:UniRef50_P75199" ] }, "pscc": { "uniref50_id": "UniRef50_P75199", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75199" ], "product": "Uncharacterized protein MPN_581" } }, "id": "CNOCLNGIAL_1235", "locus": "GCA000143945_01235" }, { "type": "cds", "sequence": "contig_1", "start": 698579, "stop": 698734, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGTATGGCAAGGGAATTGGGATCACTAATGGTAGTTTAAGTCGTGGCGCTTCGGGTTCCTTAGTCTTAAATAACAAGCGACAGATTGTGGCCATTTACTTTGCTAGTAGAATCACTGAAACACAAGAATGGGGTCTAGCGCAGCTGTTACGCTGA", "aa": "MYGKGIGITNGSLSRGASGSLVLNNKRQIVAIYFASRITETQEWGLAQLLR", "aa_hexdigest": "2591b3d16ac1c27cebacf4a224baedf9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 34, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 1.56327125151406e-10, "score": 36.63825225830078, "start": 2, "stop": 35 } ], "seq_stats": { "molecular_weight": 5540.316699999999, "isoelectric_point": 10.419847679138183 }, "id": "CNOCLNGIAL_1236", "locus": "GCA000143945_01236" }, { "type": "cds", "sequence": "contig_1", "start": 699197, "stop": 699751, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_582", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75198", "UniRef:UniRef90_P75198" ], "nt": "TTGGCACTAACTGTTTTAAGTGCTTGTTCAGCCTTTCGAAAACCTGATAAATTCGAAGGGTATTACATTAACAATATTCCTGGTAGTGCAGAAATCGATCATGCCAACTATGATTTAACCTTTTCTTTAGGTTTTTTTAACAGAATTGAAAAAACCGAGAAAGATAAAAGTCAAATAGATTTTGGTTCCATTCACGGAACGGGTTGGTTAATTGACTGGAAGGAAGTTGACAAGAAAGATAAAAAAGCTAATAAATTTACTGTTTACTTAGCTACTAATTTACACGTCATACAGGCCTTAAAAAACCGAGAAGATCATCCGCCTTACAACCAATTTGATATCAATTTTGTGCGAACCGTTGATTTTAGAATTGGCAAATACACTGATGTTAAACAATTTGTCGCACCAAGCAAATTAGGCTTACCCAACTCTACCCAAGCTTTTGTAGCAGCACAACCAACGGTACTTCCTAAAACTGCTTTCATTGCACATGACTTTGTGAACTACACTTTATCCAAAGATCAAAAGAATAAAAAACAAAGAGAACAGCAATGA", "aa": "MALTVLSACSAFRKPDKFEGYYINNIPGSAEIDHANYDLTFSLGFFNRIEKTEKDKSQIDFGSIHGTGWLIDWKEVDKKDKKANKFTVYLATNLHVIQALKNREDHPPYNQFDINFVRTVDFRIGKYTDVKQFVAPSKLGLPNSTQAFVAAQPTVLPKTAFIAHDFVNYTLSKDQKNKKQREQQ", "aa_hexdigest": "34495acd0dab58c2013c42c94769968b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 698894, "stop": 699793, "observations": { "mutation": [ 699199, 699790 ], "stop": [ 699199, 699790 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75198", "query_cov": 1.0, "subject_cov": 0.4043956043956044, "identity": 0.995, "score": 377.0, "evalue": 2.27e-128, "gene_start": 1, "gene_end": 184, "reference_start": 15, "reference_end": 198, "reference_sequence": "MWVALKRFCFLSGLLALTVLSACSAFRKPDKFEGYYINNIPGSAEIDHANYDLTFSLGFFNRIEKTEKDKSQIDFGSIHGTGWLIDWKEVDKKDKKANKFTVYLATNLHVIQALKNREDHPPYNQFDINFVRTVDFRIGKYTDVKQFVAPSKLGLPNSTQAFVAAQPTVLPKTAFIAHDFVNYTLSKDQKNKKQREQQWKVQIKPNKDEVHPYADFAVLELPLFLNNSSDRQIFDHFIQPAIRAYKQLGDSLNIFAYPTLDQFKHSHYYVLGYPYIAKKLPTLFVNQTGKEKTVPGETAQIPNDQPFVSTINEEGPHLGRIKSDKFNGGTWAWNHDNTKNFPFNRQFRGKEYQMYGKGIGITNGSLSRGASGSLVLNNKRQIVAIYFASRITETQEWGLAQLLRWKPRSVLNEEKDSVAYDLIFGNSNTKKYYAQFAKKITLTFMGKLNLRETLV" }, "paralog": false, "identity": 0.9933110367892977, "score": 605.0, "evalue": 1.33e-220, "description": "Internal stop codon at 699199, 699790. Nonsense mutation around 699199, 699790.", "psc": { "uniref90_id": "UniRef90_P75198", "product": "Uncharacterized lipoprotein MPN_582", "uniref50_id": "UniRef50_P75198", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75198", "UniRef:UniRef50_P75198", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75198", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75198" ], "product": "Uncharacterized lipoprotein MPN_582" } }, "id": "CNOCLNGIAL_1237", "locus": "GCA000143945_01237" }, { "type": "cds", "sequence": "contig_1", "start": 699868, "stop": 700011, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCTATGACTTAATCTTTGGTAACAAAGACACTAAAAAGTACTACGCTCAATTTGCTAAACAACATAAAACCCATTTGTTTGAGCAAATTAAGCAAAGCAACGACCAACAATTTACCTTTGTTAAAAACCAAAAGTCTTAA", "aa": "MAYDLIFGNKDTKKYYAQFAKQHKTHLFEQIKQSNDQQFTFVKNQKS", "aa_hexdigest": "093ceb9e08a6c6cb4bb8c2259fa78da6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5687.358000000002, "isoelectric_point": 9.574667167663574 }, "id": "CNOCLNGIAL_1238", "locus": "GCA000143945_01238" }, { "type": "cds", "sequence": "contig_1", "start": 700264, "stop": 700626, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_583", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197", "UniRef:UniRef90_P75197" ], "nt": "GTGTTAGAGCTAACCTTATTTTTAGATAACCCAGTTGATAAAAAGGTGTTTGACCATTTTATCCAACCAGCCATCAAAACTTATGAGCAATTAGGTAATAGCTTAAAGCTGTTTGCCAAGCCAACATTAACTGAACTCAAAAACCACCGCTATTATTTATCGGGTTATCCCTTCTTGGAAAACAAGGTTAATGTATTGAGTGTTAATCAAATTAAGAAAGATACATCTACAGAAACAACAATAAATGGACAAACAGTACAACAAAGTAACCACGAACAACCCCTTATTGTGCGATCGACAATAGCAAAGCCAACCCTAATTAGAAATCAAGGTGATGACTTTAATGGCAGTAACTGGGTTTGA", "aa": "MLELTLFLDNPVDKKVFDHFIQPAIKTYEQLGNSLKLFAKPTLTELKNHRYYLSGYPFLENKVNVLSVNQIKKDTSTETTINGQTVQQSNHEQPLIVRSTIAKPTLIRNQGDDFNGSNWV", "aa_hexdigest": "790d2211dd4e7a156dd27d13919386a5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 700231, "stop": 700908, "observations": { "mutation": [ 700266, 700710 ], "stop": [ 700266, 700710 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75197", "query_cov": 1.0, "subject_cov": 0.5333333333333333, "identity": 0.992, "score": 241.0, "evalue": 3.8e-79, "gene_start": 1, "gene_end": 120, "reference_start": 95, "reference_end": 214, "reference_sequence": "MPNNAQAFVNVQTTVIPKTAFTAHDFVNYTLPKDKKKQNDTENKKKQPKDGENDKQKEQAETQPFEWIQQKDADDKKESNTANTSDSLAYADFAVLELTLFLDNPVDKKVFDHFIQPAIKTYEQLGNSLKLFAKPTLTELKNHRYYLSGYPFLENKVNVLSVNQIKKDTSTETTINGQTVQQSNHEQPLIVRSTIAKPTLIRNQGDDFNGSNWVWNYDNSRVFRF" }, "paralog": false, "identity": 0.9866666666666667, "score": 448.0, "evalue": 2.3e-163, "description": "Internal stop codon at 700266, 700710. Nonsense mutation around 700266, 700710.", "psc": { "uniref90_id": "UniRef90_P75197", "product": "Uncharacterized protein MPN_583", "uniref50_id": "UniRef50_P75197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75197", "UniRef:UniRef50_P75197" ] }, "pscc": { "uniref50_id": "UniRef50_P75197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197" ], "product": "Uncharacterized protein MPN_583" } }, "id": "CNOCLNGIAL_1239", "locus": "GCA000143945_01239" }, { "type": "cds", "sequence": "contig_1", "start": 700708, "stop": 700908, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCAATAATGCTCAAGCCTTTGTTAATGTCCAAACCACAGTAATTCCCAAAACCGCTTTTACAGCGCATGACTTTGTCAACTACACTTTGCCAAAAGACAAAAAGAAGCAAAATGATACAGAAAATAAAAAGAAGCAACCAAAGGATGGAGAAAACGATAAGCAAAAGGAGCAAGCGGAAACCCAACCGTTTGAGTGA", "aa": "MPNNAQAFVNVQTTVIPKTAFTAHDFVNYTLPKDKKKQNDTENKKKQPKDGENDKQKEQAETQPFE", "aa_hexdigest": "27f00c9c5972bf2d144a27d58588c4bf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7593.327200000004, "isoelectric_point": 7.967276954650878 }, "id": "CNOCLNGIAL_1240", "locus": "GCA000143945_01240" }, { "type": "cds", "sequence": "contig_1", "start": 701103, "stop": 701348, "strand": "-", "frame": 1, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747", "UniRef:UniRef90_UPI00143DB747" ], "nt": "ATGATTGTGGCTTTCAAGCGCCTTGGTTTTCTCGCTAGCATAGCTTCCTTAACTGTATTAAGTGCTTGTGCTGCAGTGCCACTAGAAAAGTGGGATCAGTTCTATGTAAGTAATATTCCTAGTAGTGGTTCGATTCACCGTGCCAACTATGATTTAACCTTTGCTTTAACTTTTACTAATAAAACTACTAACAATGGGCAAGATAACTTTAGCGCCTTTTCTGGCACTGGCTGGCTCATTGATTGA", "aa": "MIVAFKRLGFLASIASLTVLSACAAVPLEKWDQFYVSNIPSSGSIHRANYDLTFALTFTNKTTNNGQDNFSAFSGTGWLID", "aa_hexdigest": "27a4ae5b6919c157144c26f8a16e0504", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00143DB747", "query_cov": 0.9629629629629629, "subject_cov": 0.896551724137931, "identity": 1.0, "score": 158.0, "evalue": 5.08e-49, "valid": true, "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_UPI00143DB747" }, "pscc": { "uniref50_id": "UniRef50_UPI00143DB747", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747" ], "product": "DUF31 family putative serine protease" }, "genes": [], "id": "CNOCLNGIAL_1241", "locus": "GCA000143945_01241" }, { "type": "cds", "sequence": "contig_1", "start": 701412, "stop": 701567, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTATGATTTAATCTTTGGTAATAAAAATACCACAAAATACTATGCTCAGTTTGCCAAAGAACACAAAACCCATTTATACGAGCAAATTAAGCAAATTAACAATGATGAATTCCGCTTTGTCGAAAAGACTAAAAGGCATAACTTGGGGTAA", "aa": "MAYDLIFGNKNTTKYYAQFAKEHKTHLYEQIKQINNDEFRFVEKTKRHNLG", "aa_hexdigest": "282fc9ff7f7dc1613d642b8764a0caa5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6222.954599999999, "isoelectric_point": 9.347158241271973 }, "id": "CNOCLNGIAL_1242", "locus": "GCA000143945_01242" }, { "type": "cds", "sequence": "contig_1", "start": 701604, "stop": 701819, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_584", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75196", "UniRef:UniRef90_P75196" ], "nt": "TTGAACTATGATCTCTCCCAGAAATTTAAGGTTTATCTTGAGTTTTCGGGCAAAAAGTACCGAATCTATGGCCAAAGTATAGGCATTAGTCATCTTGATTTAGGCGGTGGATCTTCTGGTTCTTTGTTGTTAAATGATAAGAAACAAATCGCTGGCATTTACTTTGGCGTTGATGGTGCTAACAATGAATTAGGCTTAGCACAGTTATTACGCTGA", "aa": "MNYDLSQKFKVYLEFSGKKYRIYGQSIGISHLDLGGGSSGSLLLNDKKQIAGIYFGVDGANNELGLAQLLR", "aa_hexdigest": "1aa1fda7670c3abf5a06f733d472adf5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 701412, "stop": 701819, "observations": { "mutation": [ 701606 ], "stop": [ 701606 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75196", "query_cov": 1.0, "subject_cov": 0.5259259259259259, "identity": 1.0, "score": 141.0, "evalue": 5.23e-42, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MNYDLSQKFKVYLEFSGKKYRIYGQSIGISHLDLGGGSSGSLLLNDKKQIAGIYFGVDGANNELGLAQLLRWKPETYHNDEARSVAYDLIFGNKNTTKYYAQFVKEHKTHLYEQIKQINNDEFRFVEKTKRHNLG" }, "paralog": false, "identity": 0.9777777777777777, "score": 271.0, "evalue": 2.21e-95, "description": "Internal stop codon at 701606. Nonsense mutation around 701606.", "psc": { "uniref90_id": "UniRef90_P75196", "product": "Uncharacterized protein MPN_584", "uniref50_id": "UniRef50_P75196", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75196", "UniRef:UniRef50_P75196", "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P75196", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75196" ], "product": "Uncharacterized protein MPN_584" } }, "id": "CNOCLNGIAL_1243", "locus": "GCA000143945_01243" }, { "type": "cds", "sequence": "contig_1", "start": 701905, "stop": 702324, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_585", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75195", "UniRef:UniRef90_P75195" ], "nt": "GTGCAAACAAGTGTAATTCCCAAAACCGCTTTTGCTGCTAGGGACTTTGTGGATTACAGTTTTCCTCAAGAACAAAAAGATAAAGAAAAGAGAAAACAACAATGGGTGAAAAACAGTCATACTAAGAATTCTGATGTCCAGCCTTTTGCTGAGTTTGCAATTTTAGAAATTCCCTTATTTTCGAAAAGTAAAGTAGATCGCAAAATCTTTGATCACTTTATTCAACCAGCCATACGCACTTATAAACAGTTAGGTGATAGCCTTAACATTTTTGCTAACCCCACACTGGATCAATTAAAACAAAATCGCTATTATGTGTTAGGTTATCCGTTTTTAAAAAATAAAGTGTCATCACTATTTCTTAACCAAACGGGAAAAAAGAAAGGAATATTTAGGCGAAAATACACAAATATCCCATGA", "aa": "MQTSVIPKTAFAARDFVDYSFPQEQKDKEKRKQQWVKNSHTKNSDVQPFAEFAILEIPLFSKSKVDRKIFDHFIQPAIRTYKQLGDSLNIFANPTLDQLKQNRYYVLGYPFLKNKVSSLFLNQTGKKKGIFRRKYTNIP", "aa_hexdigest": "a34ffbf4fb9390d6be9e771e5c387c7e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 701881, "stop": 702624, "observations": { "mutation": [ 701907, 702423, 702552, 702564 ], "stop": [ 701907, 702423, 702552, 702564 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75195", "query_cov": 1.0, "subject_cov": 0.4602649006622517, "identity": 0.986, "score": 274.0, "evalue": 8.62e-91, "gene_start": 1, "gene_end": 139, "reference_start": 156, "reference_end": 294, "reference_sequence": "MLVVFKRLGFIVSIFSLTFLSACAAFDKWQEFYINNIPSSTEIHRFNYDLNFSLTFTNRITSNGEAKFSVTYGTGWLIDWKEPDKKKENDPFRAYLATNLHVAASLINPQDYEPYKNKDDAGWTTTFRLGKYTKVSDFVSPNQFGLPNAAQALVNVQTSVIPKTAFAARDFVDYSFPQEQKDKEKRKQQWVKNSHTKNSDVQPFAEFAILEIPLFSKSKVDRKIFNHFIQPAIRTYKQLGDSLNIFANPTLDQLKQNRYYVLGYPFLKNKVSSLFLNQTGKKKGIFRRKYTNIPWKTSYYLN" }, "paralog": false, "identity": 0.9757085020242915, "score": 483.0, "evalue": 2.21e-175, "description": "Internal stop codon at 701907, 702423, 702552, 702564. Nonsense mutation around 701907, 702423, 702552, 702564.", "psc": { "uniref90_id": "UniRef90_P75195", "product": "Uncharacterized lipoprotein MPN_585", "uniref50_id": "UniRef50_P75195", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75195", "UniRef:UniRef50_P75195", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75195", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75195" ], "product": "Uncharacterized lipoprotein MPN_585" } }, "id": "CNOCLNGIAL_1244", "locus": "GCA000143945_01244" }, { "type": "cds", "sequence": "contig_1", "start": 702562, "stop": 702789, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGTTGTTTTCAAGCGTCTTGGTTTTATTGTTAGCATATTCTCTTTAACTTTTTTAAGTGCTTGTGCAGCCTTTGATAAGTGGCAAGAGTTTTACATTAACAATATCCCTAGTAGTACGGAAATCCACCGATTCAACTATGACTTAAACTTCTCGCTTACTTTTACTAATAGAATCACTAGCAACGGTGAAGCTAAATTTAGTGTCACATATGGTACTGGTTGA", "aa": "MLVVFKRLGFIVSIFSLTFLSACAAFDKWQEFYINNIPSSTEIHRFNYDLNFSLTFTNRITSNGEAKFSVTYGTG", "aa_hexdigest": "a220d5ac26ea612ca60edbad7584f6ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8588.689499999993, "isoelectric_point": 7.907579231262207 }, "id": "CNOCLNGIAL_1245", "locus": "GCA000143945_01245" }, { "type": "cds", "sequence": "contig_1", "start": 703259, "stop": 703852, "strand": "-", "frame": 2, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197", "UniRef:UniRef90_UPI0006BA639D" ], "nt": "GTGGATTTTAGACTGGGCAAATATACTAACGTGAAAGACTTTGTGTCACCAAACGAAATAGGATTACCTAATGGTGCACAAGCCTTTGTATCAGCGCAGACAAGCACAATTCCCAAAACGGCTTTCACTGCGCACGATTTTGTGGATTACACCTTGCCCCAAGAACAAAAAGGTAAAGAACAAAAGGAGCAGCAGTGGATGAAGAACAGTCAAACTAAGGATACTGATGTTCATCCCTATGCTGATTTTGCAGTCTTAGAAATCCTTTTGTTTCCTGACAGTTCTACTGATCGCAAAATTTTTAATCACTTTATCCAACCTGCCATTAGAGCCTATAAACAATTAGGTGATAGCTTAAATATCTTTGCTAATCAAACCCTAGATCAACCTAAACATAACCGTTATTACTTGTTGGGTTATCCTTTTTTAAGAAATAAAGCGGCATCATTGTTTTTAAATCAAACGGAACAAAGAAAGGAACATATGGACAAAACTACCCAAATACTTCATGAAAAACCATTTGTTGTTTTAACCGATCCAGCTAAGCCTACTTTAATTAGGAATAAAGGCGCTAATTTTACTGGCAACACTTGA", "aa": "MDFRLGKYTNVKDFVSPNEIGLPNGAQAFVSAQTSTIPKTAFTAHDFVDYTLPQEQKGKEQKEQQWMKNSQTKDTDVHPYADFAVLEILLFPDSSTDRKIFNHFIQPAIRAYKQLGDSLNIFANQTLDQPKHNRYYLLGYPFLRNKAASLFLNQTEQRKEHMDKTTQILHEKPFVVLTDPAKPTLIRNKGANFTGNT", "aa_hexdigest": "7f53251c9e47c7983872e09205f14340", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0006BA639D", "query_cov": 0.9086294416243654, "subject_cov": 0.8861386138613861, "identity": 0.9940000000000001, "score": 360.0, "evalue": 3.41e-125, "valid": true, "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_P75197" }, "pscc": { "uniref50_id": "UniRef50_P75197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197" ], "product": "Uncharacterized protein MPN_583" }, "genes": [], "id": "CNOCLNGIAL_1246", "locus": "GCA000143945_01246" }, { "type": "cds", "sequence": "contig_1", "start": 703979, "stop": 704233, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_587", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75193", "UniRef:UniRef90_P75193" ], "nt": "ATGGTAATTGCCTTAAAGCGGTTTAGTTTTCTAGCTAGCATAGCTACATTAACTGTGCTCAACGCTTGTGCAACAATTTCACTATCTGATAAATCTGGTGAGTTTTACATTAACAATATTCCTAGTAGTGCTGAATTACACCGGATTAACTATGACTTGACCTTCTCTTTAGGTTTTACCAACAGAATTCAGACTAAAAAGAAGAATGAAGTAAACTTCGTTGCCTTTTATGGTACTGGTTGGTTGATTGACTGA", "aa": "MVIALKRFSFLASIATLTVLNACATISLSDKSGEFYINNIPSSAELHRINYDLTFSLGFTNRIQTKKKNEVNFVAFYGTGWLID", "aa_hexdigest": "48ecbadbc1ae91162ddd6413354b574a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 703782, "stop": 704233, "observations": { "deletion": [ 703795 ], "mutation": [ 703858, 703981 ], "stop": [ 703858, 703981 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75193", "query_cov": 1.0, "subject_cov": 0.56, "identity": 0.988, "score": 159.0, "evalue": 1e-48, "gene_start": 1, "gene_end": 84, "reference_start": 1, "reference_end": 84, "reference_sequence": "MVIALKRFSFLASIATLTVLNACATISLSDKSGEFYINNIPSSAELHRINYDLTFSLGFTNRIQTKKKNEVNFVAFYGTGLLIDWKEGNQYQPFRVYLATNVHVAAGLKNEADYLPYSQPDAELGWTVDFRLGKYTNVKDFVSPNENRIT" }, "paralog": false, "identity": 0.98, "score": 285.0, "evalue": 1.4e-100, "description": "Internal stop codon at 703858, 703981. Frameshift due to deletion around 703795. Nonsense mutation around 703858, 703981.", "psc": { "uniref90_id": "UniRef90_P75193", "product": "Uncharacterized lipoprotein MPN_587", "uniref50_id": "UniRef50_P75193", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75193", "UniRef:UniRef50_P75193", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75193", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75193" ], "product": "Uncharacterized lipoprotein MPN_587" } }, "id": "CNOCLNGIAL_1247", "locus": "GCA000143945_01247" }, { "type": "cds", "sequence": "contig_1", "start": 704291, "stop": 704737, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "GTGATCCAAGACAAAGGTGTGGTGAACTTTGCTTCCTTTAGCAACTTTACCTTGAAGTACCACGAAAAGCGGTTCCAACAATACGGCTATGGCTTAATGTTGAATGACACTAATTTTCCGGGTGGCTCTTCGGGTAGTCCTTTAATTGGTAAAGACAATAAATTGAACAGCATTTACTTTGGGGTGTTGGAAATTTACCAATCTGGTAGTCTTGCACGCAATGACATTGGGATGAGCCAAATCTTACGTACTCCACAGAACGATAAAGGTTCAAGTATTTCTAAGGGTAGCTATGACTTGATCTTTGGCGATAAGAACACCAAGAATTATTACGCTAAGTTTGCTAAAGATCATCAAACTCATTTGTATCAGAAGATTAAAGAAAGCAAAGACGAACAATTCCGCTTTGTGGAAGCACAAGAGACAACTAATAATTTAGGCAATTAA", "aa": "MIQDKGVVNFASFSNFTLKYHEKRFQQYGYGLMLNDTNFPGGSSGSPLIGKDNKLNSIYFGVLEIYQSGSLARNDIGMSQILRTPQNDKGSSISKGSYDLIFGDKNTKNYYAKFAKDHQTHLYQKIKESKDEQFRFVEAQETTNNLGN", "aa_hexdigest": "aa26f1b80ff1548c4b7791f553cbd8d1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 51, "aa_cov": 0.34459459459459457, "hmm_cov": 0.8823529411764706, "evalue": 5.410039949195413e-15, "score": 51.307559967041016, "start": 11, "stop": 61 } ], "seq_stats": { "molecular_weight": 16789.508199999986, "isoelectric_point": 8.981106758117676 }, "id": "CNOCLNGIAL_1248", "locus": "GCA000143945_01248" }, { "type": "cds", "sequence": "contig_1", "start": 704873, "stop": 705886, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_588", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q50339", "UniRef:UniRef90_Q50339" ], "nt": "ATGAGACTGCAATTCAAGTTATTGGGTTTCTTAACACTGCTGGGTACCAGCACTATTCTTAGTGCCTGTGCTGCAACCCAGCCAAACTTTGAACCCAATAACATTGAAGAAAGTGGTCCCATTACACCTACCACACCTACAACTGATGTACCTAAACCAACCGCAGAGGTAGTGCCTGTTAACCGTGGCTTTCACTTCCAAACCAATAAGGTACCTAGTGAAAGTGATGTTTTTAAGCATAACTATGATCTAACTTTTTCGTTGAACTTTACCAATAAGTCTAACGATATCTATGGCACCGGCTGGTTATTTGACTGGAAGGGTGATGAAAAGGCATTAGGTATTGATGGCAGTTTTGTCCCTAGCATTACAAGCAACATTGATAACTCCCTTCTAAAGGATGATCAATTCACCGTTTATTTAGCCACTAACTTACACGTAGCTGATGCTTTAAGGAATGATCAGGATTACGAACCCTACAAAAAAGATCAGAACAAGCAAGATTTTACTGAGAATACTAAAACTGAATTCTTTTCTTTGGGTAAATACTTAGAAGGTGAGCAGTTAAAGCAGTACATTTCTAAAGAAGAAAATCAATCAGCCAATCAAACTGATAAAGCCTTGGTTTCGATTCAGGCCTCCAATATTCCCAAAACGGCTTACACTGCTACTGACTTTGTTGATATGAATAGTTACAGTTATAACAACATCACAACAAGTCTTCCTGGCAACTATGCTGACTTTGCCGTGATTGAAGTTAACCTAAACCTTAAAAATCAACGCGATCAGCAAATTCTTCATGACTTTGTCAAACCAGCAATTAAGGCTTACAAGGCTTTAGGTGATACTTTGGAACTGTTTAGTGCCAAACCACTCAACCAGTTCATCGAACAAAACTACTACTTGTTAGGTTATCCAGTGATTAATAAAGGAAACAATACCGCTAACCTTTTATTAGCGCAACAAAAGAGCTTTGATCACAACAATTCGGATCATAACCAAAAGAGCCAGTGA", "aa": "MRLQFKLLGFLTLLGTSTILSACAATQPNFEPNNIEESGPITPTTPTTDVPKPTAEVVPVNRGFHFQTNKVPSESDVFKHNYDLTFSLNFTNKSNDIYGTGWLFDWKGDEKALGIDGSFVPSITSNIDNSLLKDDQFTVYLATNLHVADALRNDQDYEPYKKDQNKQDFTENTKTEFFSLGKYLEGEQLKQYISKEENQSANQTDKALVSIQASNIPKTAYTATDFVDMNSYSYNNITTSLPGNYADFAVIEVNLNLKNQRDQQILHDFVKPAIKAYKALGDTLELFSAKPLNQFIEQNYYLLGYPVINKGNNTANLLLAQQKSFDHNNSDHNQKSQ", "aa_hexdigest": "cdd50434066eedfcc3904d9d1939c39a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 704570, "stop": 705886, "observations": { "mutation": [ 704875 ], "stop": [ 704875 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50339", "query_cov": 1.002967359050445, "subject_cov": 0.6353383458646616, "identity": 0.997, "score": 654.0, "evalue": 8.66e-234, "gene_start": 1, "gene_end": 337, "reference_start": 1, "reference_end": 338, "reference_sequence": "MRLQFKLLGFLTLLGTSTILSACAATQPNFEPNNIEESGPITPTTPTTDVPKPTAEVVPVNRGFHFQTNNKVPSESDVFKHNYDLTFSLNFTNKSNDIYGTGWLFDWKGDEKALGIDGSFVPSITSNIDNSLLKDDQFTVYLATNLHVADALRNDQDYEPYKKDQNKQDFTENTKTEFFSLGKYLEGEQLKQYISKEENQSANQTDKALVSIQASNIPKTAYTATDFVDMNSYSYNNITTSLPGNYADFAVIEVNLNLKNQRDQQILHDFVKPAIKAYKALGDTLELFSAKPLNQFIEQNYYLLGYPVINKGNNTANLLLAQQKSFDHNNSDHNQKSQWFTKDQSYINKLDRIPVLTNNYRAYNESTGSQLFANQQNESWLNGVVIQDKGVVNFASFSNFTLKYHEKRFQQYGYGLMLNDTNFPGGSSGSPLIGKDNKLNSIYFGVLEIYQSGSLARNDIGMSQILRTPQNDKGSSISKGSYDLIFGDKNTKNYYAKFAKDHQTHLYQKIKESKDEQFRFVETQETTNNLGN" }, "paralog": false, "identity": 0.9954441913439636, "score": 882.0, "evalue": 0.0, "description": "Internal stop codon at 704875. Nonsense mutation around 704875.", "psc": { "uniref90_id": "UniRef90_Q50339", "product": "Uncharacterized lipoprotein MPN_588", "uniref50_id": "UniRef50_Q50339", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50339", "UniRef:UniRef50_Q50339", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q50339", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50339" ], "product": "Uncharacterized lipoprotein MPN_588" } }, "id": "CNOCLNGIAL_1249", "locus": "GCA000143945_01249" }, { "type": "cds", "sequence": "contig_1", "start": 706102, "stop": 706479, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_589", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "SO:0001217", "UniRef:UniRef50_Q50338", "UniRef:UniRef90_Q50338" ], "nt": "ATGCCAACACTTACCAAAAACTTAAGGCAAGGTCAACCAGTTGCTGGTAGTAAAGTGCACACATATTACAGTAACGGCTTCCTTAATGAATATTCCTTAAATCAAGGAATTGTTGATTTTGGTACTTTTAAAAGTGCTATCACTGATTATCATGGATATGAATATAAAAACCACGGCTATGGCTTAGCTCTAACTGATACTGATTTACTAGGTGGATCGAGTGGCAGTTTAGTGTTTAACCAAGACAAAAAGATTAGCAGTATTTATTCAGCTGCTACTGAATCAGATAGTGTTGGCTATGCCCAGCTATTGCCTGTTCCTAAAGATGTTAATGGGGTAAGTTTAGTTAAATACAGTTACGACCTTATACTTTGGTGA", "aa": "MPTLTKNLRQGQPVAGSKVHTYYSNGFLNEYSLNQGIVDFGTFKSAITDYHGYEYKNHGYGLALTDTDLLGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLPVPKDVNGVSLVKYSYDLILW", "aa_hexdigest": "87242a62519f9e884e9deda655e90b23", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 706102, "stop": 706572, "observations": { "mutation": [ 706527 ], "stop": [ 706527 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50338", "query_cov": 1.0, "subject_cov": 0.7961783439490446, "identity": 0.992, "score": 250.0, "evalue": 1.06e-83, "gene_start": 1, "gene_end": 125, "reference_start": 32, "reference_end": 156, "reference_sequence": "MGYPTLWTDDAKLFEWTKTEQRFNHDDFYGSMPTLTKNLRQGQPVAGSKVHTDYSNGFLNEYSLNQGIVDFGTFKSAITDYHGYEYKNHGYGLALTDTDLLGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLPVPKDVNGVSLVKYSYDLILWW" }, "paralog": false, "identity": 0.9807692307692307, "score": 315.0, "evalue": 7.78e-112, "description": "Internal stop codon at 706527. Nonsense mutation around 706527.", "psc": { "uniref90_id": "UniRef90_Q50338", "product": "Uncharacterized protein MPN_589", "uniref50_id": "UniRef50_Q50338", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50338", "UniRef:UniRef50_Q50338", "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_Q50338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50338" ], "product": "Uncharacterized protein MPN_589" } }, "id": "CNOCLNGIAL_1250", "locus": "GCA000143945_01250" }, { "type": "cds", "sequence": "contig_1", "start": 706713, "stop": 706961, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "GTGAATGATTATGAGTTTTTCAATCAATTTGATTACTTTGACGATCCAACTGAATCTTTCACTTTAGGTAAGTTCGTTGATGGTAATGAAATAAAGCAAATAGTTCCTGATGCTATGCATGAACCTAGCTTAGTGCGGATTGAAACTTCAAAATTGCCAAAAACTGCTTACACGGCAACACTTTTTATTAATGATTTGGGGGAATATAGATTACCTGCCGCTGACTTTGCTGTATTGGAATCAATCTAG", "aa": "MNDYEFFNQFDYFDDPTESFTLGKFVDGNEIKQIVPDAMHEPSLVRIETSKLPKTAYTATLFINDLGEYRLPAADFAVLESI", "aa_hexdigest": "8b3e499ae1f978cc3789f34561e0ef49", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 95, "aa_cov": 0.926829268292683, "hmm_cov": 1.0, "evalue": 1.3047761003072088e-07, "score": 27.030988693237305, "start": 5, "stop": 80 } ], "seq_stats": { "molecular_weight": 9402.401499999996, "isoelectric_point": 4.195479393005371 }, "id": "CNOCLNGIAL_1251", "locus": "GCA000143945_01251" }, { "type": "cds", "sequence": "contig_1", "start": 707037, "stop": 707258, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGTAAACAACAAAAGTGTTAGTGCAGTTCAACCAGAAGACAAGCAAGCATTAAAACCAGACCCTGCTTTAGGTGAATACACCTCTGACTTAAAGTTCAATACTAACTTCATTCCAAAAAAGGATGATCCCTTTCGTCCCTTTTATGATCTAAGTTTTACGCTCCAGTTTCAAGATCCATATACAGCTACTTACGGTACTGGTTGGCTCATTGATTGA", "aa": "MPVNNKSVSAVQPEDKQALKPDPALGEYTSDLKFNTNFIPKKDDPFRPFYDLSFTLQFQDPYTATYGTGWLID", "aa_hexdigest": "e8bd5c455fb50bb570a386a0bfaa2cff", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8334.204199999996, "isoelectric_point": 4.611427116394043 }, "id": "CNOCLNGIAL_1252", "locus": "GCA000143945_01252" }, { "type": "cds", "sequence": "contig_1", "start": 707523, "stop": 708584, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_591", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "RefSeq:WP_010874948.1", "SO:0001217", "UniParc:UPI000013ADAA", "UniRef:UniRef100_Q50336", "UniRef:UniRef50_Q50336", "UniRef:UniRef90_Q50336" ], "nt": "GTGGCTAACAGCTTAAGGAACGTTAATGATTACGATCCTTTTAAATACCGCCCCAGTTATTTTGATCTAGAGGCACCAACCGAATCATTTGCTTTAGGTAAGTTTGTCGATGCGATTGAAGTTAAGCAGATGGCACTAGATGCCTTTAGTGAATCTAGCATGGTCAGTATTGCAACTTCACAGCTACCCAAAACAGCTTACACAGCTACAAGTTTTATTGAAAGCGAGTATTATAGTTTTCCTTATTTATTTAACGACCAAAAATATTACTGGGATTATTTTGATTACAAAATCCCTGCCGCTGACTTTGCCGTATTAGAGTTAGAACTGGATTTAAACAACCAGCAGGATCAGCAAATTAAAGATCATTTCATCGATCCAGCGATCAAGGCTTATAAACAATTAGGTGATAGTACCGGTTTGTTTGCCACCAAACCATTAACTGAATATCAAAACGATACCCATTACCTCTTGGGCTATCCGGTTGTACCTACTGATCATACACAACTGTGGGAATGCAAACAAGGGGCAGAGCGCTTTAGCTATGGTTATTTTTACAGTAATATGGCTAGACTAACCAAAAACCTAAGGCAGGGCGATCCAAACGCTGGTAGTAAGACACACATCGAATACAGCAATGAGTTACTCGATAAGGACTCAATGGATCAAGGTATTGTGCGTTTTAGTACCTTTTTGGGTGCTAACATTAATTACCACGATTACGATTACCGTCAACAGGGTTACGGTTTAACCTTAACCGACACTAACTTGCCAGGTGGATCGAGTGGCAGTTTAGTGTTTAACCAAGACAAAAAGATTAGCAGTATTTATTCAGCTGCTACTGAATCAGATAGTGTTGGCTATGCTCAGTTATTACGAACTCCTAGGGATGTCAACGGTATAAGTGTAGTTTCACAAAGCTATGACCTCATCTTTGGTGATAGCAACACTAAGCGTTACTATGCGATGTTTGCTAAAAAACAGCAAACTCACTTGTACAGTGAAATCCTTAAAAGTACTGATGAACAGTACCGTTATGTGGTAGACAAACAATTCAATTAA", "aa": "MANSLRNVNDYDPFKYRPSYFDLEAPTESFALGKFVDAIEVKQMALDAFSESSMVSIATSQLPKTAYTATSFIESEYYSFPYLFNDQKYYWDYFDYKIPAADFAVLELELDLNNQQDQQIKDHFIDPAIKAYKQLGDSTGLFATKPLTEYQNDTHYLLGYPVVPTDHTQLWECKQGAERFSYGYFYSNMARLTKNLRQGDPNAGSKTHIEYSNELLDKDSMDQGIVRFSTFLGANINYHDYDYRQQGYGLTLTDTNLPGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLRTPRDVNGISVVSQSYDLIFGDSNTKRYYAMFAKKQQTHLYSEILKSTDEQYRYVVDKQFN", "aa_hexdigest": "e61db966f3ccde618ac15ccaaa1edfdf", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ADAA", "ncbi_nrp_id": "WP_010874948.1", "uniref100_id": "UniRef100_Q50336", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ADAA", "RefSeq:WP_010874948.1", "UniRef:UniRef100_Q50336" ] }, "ips": { "uniref100_id": "UniRef100_Q50336", "uniref90_id": "UniRef90_Q50336" }, "psc": { "uniref90_id": "UniRef90_Q50336", "product": "Uncharacterized protein MPN_591", "uniref50_id": "UniRef50_Q50336", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_Q50336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50336" ], "product": "Uncharacterized protein MPN_591" }, "genes": [], "id": "CNOCLNGIAL_1253", "locus": "GCA000143945_01253" }, { "type": "cds", "sequence": "contig_1", "start": 709107, "stop": 709562, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGGTACAAAATCAAATTAACTATTTACCCTTTGCACAAAATGACCCTAAAGGCGTAATGGACTTTAGCAAGTATTTCAATTACGTTTTTAACTATCACGAAAAGCAATACCAACACCATGGTTATGGTTTATTGTTAGAAGACACTGACTTCCCGGGTGGTTCCAGTGGTAGTCCGTTATTTAACCAAAACAAACAGATTAACAGTATTTACTTTGCGGCTTTACCAAGTAAAAGTTACGGTGTATCGCAAATTCTCCGCGCTACGCAAAACAAAGACAAGTCCAAAAACTACGATTTAATCTTTGGTGATAGCAACACCAAGAAATACTATGCTCAATTTGCTAAGGAGCACAAAACCCACTTGTACCATCAAATTCTTCAAAGTAACGATGAACAGTTCCGCTTTGTCGAAAACGATCAAACTGTAACAAGTCAAACACCATTTAAAAGCTAA", "aa": "MVQNQINYLPFAQNDPKGVMDFSKYFNYVFNYHEKQYQHHGYGLLLEDTDFPGGSSGSPLFNQNKQINSIYFAALPSKSYGVSQILRATQNKDKSKNYDLIFGDSNTKKYYAQFAKEHKTHLYHQILQSNDEQFRFVENDQTVTSQTPFKS", "aa_hexdigest": "cdad11514fddf82b12edb7cacff46c9e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 71, "aa_cov": 0.47019867549668876, "hmm_cov": 0.9154929577464789, "evalue": 1.9439783092203129e-16, "score": 56.05778121948242, "start": 3, "stop": 73 } ], "seq_stats": { "molecular_weight": 17603.255599999982, "isoelectric_point": 7.887013816833496 }, "id": "CNOCLNGIAL_1254", "locus": "GCA000143945_01254" }, { "type": "cds", "sequence": "contig_1", "start": 709671, "stop": 710114, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGGGTAAATACATTGATGCCCAAACTGTGCAAAAGTTAACTCCAGAGTACCAAAATCTTAAGCACCTCAATAACCGGAATAGTGATGCATTAGTTTCAATTCAAACATCGAAGTTACCAAAAACTGCTTACACTGCCACTGACTTTATCAAAACTGCTCAGTACAAATACAATCACATAGTGGGTAACACAGTTTATGAGTTGGACTTATTCCAAAATGCCGTAAGTTACGCTGACTTTGCGGTGTTGGAATTAGAGTTAAATTTAGCCAACAATCGTGACCAGCAAATCTTTGACAGTTTTATAAATCCAGCGGTAACTGCTTACGAAAAGCTAGGTAACAGTTTGGGTCTCTTTTCCAACCTGCAATTAGATCAGTATGTTGATGATACCCATTATCTATTGGGTTATCCGTTACTTAAACGTGAAAAGACGTCTTACTGA", "aa": "MGKYIDAQTVQKLTPEYQNLKHLNNRNSDALVSIQTSKLPKTAYTATDFIKTAQYKYNHIVGNTVYELDLFQNAVSYADFAVLELELNLANNRDQQIFDSFINPAVTAYEKLGNSLGLFSNLQLDQYVDDTHYLLGYPLLKREKTSY", "aa_hexdigest": "740deee53a82aef4ed3d3f5766ff4a13", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 152, "aa_cov": 0.9659863945578231, "hmm_cov": 0.9473684210526315, "evalue": 1.267900497875135e-30, "score": 102.7066879272461, "start": 2, "stop": 143 } ], "seq_stats": { "molecular_weight": 16841.695399999997, "isoelectric_point": 5.521191978454589 }, "id": "CNOCLNGIAL_1255", "locus": "GCA000143945_01255" }, { "type": "cds", "sequence": "contig_1", "start": 710304, "stop": 710672, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGTTTAAACTCAAGGGTTTTGGTTTTCTCACACTGTTCGCCAGTCAAGCATTTCTAACTGCTTGCTCTGCTACGCTAACAGTAGCTAACACTAATCACAAAAATGAAAGTGATAAGTTTGTGATTTTTGAACCTCAACCACCATTGAGCCAAACTATCCCCAAACCAGAGGCTGAACCAGTTATAGAGCCCGATGCTGTTGCTACACCACCTGTGCAGAATGCCGAGGTGCAAATTAAGCCTGACAGCTCCAAGGGTGTTTACAGTCCTGGTTTTAAGTTCAACACTAACTTTATTCCTAAAGTAAATACTAAGTATCGGCCGGGCTATGATCTTAGCTTTGCCTTAAAGTTTGGTACTAGTTGA", "aa": "MGFKLKGFGFLTLFASQAFLTACSATLTVANTNHKNESDKFVIFEPQPPLSQTIPKPEAEPVIEPDAVATPPVQNAEVQIKPDSSKGVYSPGFKFNTNFIPKVNTKYRPGYDLSFALKFGTS", "aa_hexdigest": "b9906f6d0939d177fcf7279089c58415", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13317.044699999979, "isoelectric_point": 8.709501457214355 }, "id": "CNOCLNGIAL_1256", "locus": "GCA000143945_01256" }, { "type": "cds", "sequence": "contig_1", "start": 710773, "stop": 711141, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_593", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874950.1", "SO:0001217", "UniParc:UPI000013ADB0", "UniRef:UniRef100_Q50334", "UniRef:UniRef50_Q50334", "UniRef:UniRef90_Q50334" ], "nt": "ATGAACAAAAAAGAAAGCACTACCACTAAAAAGCAGTGGTTTAAAAAGTGTTCTTTTAAGAAGTTAAAAGCGGAAATTTGCAACATGTTGCCAACAACGCCGCACAACACCAAGCGCACCTTAATCTGGGTGATTGTTTTTAGCTTTATTACTTTTCTTAGTTTTATTTTTGCTTATGTTTGCTTTAACTATGCTCCAGTATCCACCGGTTTCTTGTACTTCTTAGGAGCAGTGTTTTTACTAATTGGCTTTGCCTTTGCTATCTTGAGTTTTGTAGCAATGGTTAAATTTGTGGCTGATTATTTTGCCAATCGTTTTAGTAACACTCAGCTCAAAATGGACTGTGATTGTGCTAAAACGAAAAAGTAA", "aa": "MNKKESTTTKKQWFKKCSFKKLKAEICNMLPTTPHNTKRTLIWVIVFSFITFLSFIFAYVCFNYAPVSTGFLYFLGAVFLLIGFAFAILSFVAMVKFVADYFANRFSNTQLKMDCDCAKTKK", "aa_hexdigest": "e886a167d041086ca03c2e9933835d60", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ADB0", "ncbi_nrp_id": "WP_010874950.1", "uniref100_id": "UniRef100_Q50334", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ADB0", "RefSeq:WP_010874950.1", "UniRef:UniRef100_Q50334" ] }, "ips": { "uniref100_id": "UniRef100_Q50334", "uniref90_id": "UniRef90_Q50334" }, "psc": { "uniref90_id": "UniRef90_Q50334", "product": "Uncharacterized protein MPN_593", "uniref50_id": "UniRef50_Q50334", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q50334", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50334" ], "product": "Uncharacterized protein MPN_593" }, "genes": [], "id": "CNOCLNGIAL_1257", "locus": "GCA000143945_01257" }, { "type": "cds", "sequence": "contig_1", "start": 711367, "stop": 711669, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_594", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniParc:UPI0001DD643B", "UniRef:UniRef100_P75191", "UniRef:UniRef50_P75191", "UniRef:UniRef90_P75191" ], "nt": "ATGAGAGGGAAAAATAGGAATAAATGCAACAGCATTATTTTTTTAACAGTTGGTTTACTGTTGTTTATAGCAGCTTTAGCGCTTGGTGTATTAGTACTTTTTAACGGGTATCAGGTTAATGTCAATGCTAATGGAGTTGACTTGAAACCCTTTGAGATTGTGCATTTTCCATTTGCGGTCAAAACTTTTCTTTCTGTTTTAACCTTTGTGTTAGCAGCCTTTGGTTTTGTGTGTATGGTAGCGAGTTTTCTCTACTTTGTCAGTTTTAAGAAGCTTAAACCAAAGGCTAATTCTGCTAGTTAG", "aa": "MRGKNRNKCNSIIFLTVGLLLFIAALALGVLVLFNGYQVNVNANGVDLKPFEIVHFPFAVKTFLSVLTFVLAAFGFVCMVASFLYFVSFKKLKPKANSAS", "aa_hexdigest": "652f9e3e0df54c69fe055bbf982575e8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD643B", "uniref100_id": "UniRef100_P75191", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD643B", "UniRef:UniRef100_P75191" ] }, "ips": { "uniref100_id": "UniRef100_P75191", "uniref90_id": "UniRef90_P75191" }, "psc": { "uniref90_id": "UniRef90_P75191", "product": "Uncharacterized protein MPN_594", "uniref50_id": "UniRef50_P75191", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75191", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75191" ], "product": "Uncharacterized protein MPN_594" }, "genes": [], "id": "CNOCLNGIAL_1258", "locus": "GCA000143945_01258" }, { "type": "cds", "sequence": "contig_1", "start": 711653, "stop": 712111, "strand": "-", "frame": 2, "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "db_xrefs": [ "COG:COG0698", "COG:G", "EC:5.3.1.6", "GO:0004751", "GO:0009052", "GO:0019316", "KEGG:K01808", "RefSeq:WP_010874952.1", "SO:0001217", "UniParc:UPI000013973D", "UniRef:UniRef100_P53527", "UniRef:UniRef50_P47636", "UniRef:UniRef90_P53527" ], "nt": "ATGCAAATGAACCATCCAATTTACATTGCTTCTGACCACACTGGTTTAGAGCTCAAAAGCTTAGTGATAAAACACTTAGAACAACAAAAGTTGCAAGTGATTGATTTAGGTCCTACAGAGCTAGATCCACTTGATGACTACCCGGACTATGCCTTTTTGTTAGCGCAAACAATGCAAGCTAATCCTAATAGTTTAGGCATCCTTATTTGTGGTACTGGTGTGGGTGTTTGTATGGCCGCTAACAAGGCTAAGGGTATCTTAGCTGCTTTAGTGGTAGACAGTAAAACAGCAGCATTGGCACGCCAACATGATGACGCCAATGTGCTGTGTTTGTCTAGCCGCTTTGTGGTTCCTGAAGAAAACATTAAAATAGTAGATGAATTTCTCCAGGCGCAGTTTGAGGGTGGGCGCCATAGTAAACGTGTCGGTAAGATCATTGCTTATGAGAGGGAAAAATAG", "aa": "MQMNHPIYIASDHTGLELKSLVIKHLEQQKLQVIDLGPTELDPLDDYPDYAFLLAQTMQANPNSLGILICGTGVGVCMAANKAKGILAALVVDSKTAALARQHDDANVLCLSSRFVVPEENIKIVDEFLQAQFEGGRHSKRVGKIIAYEREK", "aa_hexdigest": "6b3e3d8771c22daa47888aaef1965a1e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013973D", "ncbi_nrp_id": "WP_010874952.1", "uniref100_id": "UniRef100_P53527", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013973D", "RefSeq:WP_010874952.1", "UniRef:UniRef100_P53527" ] }, "ips": { "uniref100_id": "UniRef100_P53527", "uniref90_id": "UniRef90_P53527" }, "psc": { "uniref90_id": "UniRef90_P53527", "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "ec_ids": [ "5.3.1.6" ], "uniref50_id": "UniRef50_P47636", "cog_id": "COG0698", "cog_category": "G", "kegg_orthology_id": "K01808", "go_ids": [ "GO:0004751", "GO:0009052", "GO:0019316" ] }, "pscc": { "uniref50_id": "UniRef50_P47636", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47636" ], "product": "Probable ribose-5-phosphate isomerase B" }, "genes": [ "rpiB" ], "id": "CNOCLNGIAL_1259", "locus": "GCA000143945_01259" }, { "type": "cds", "sequence": "contig_1", "start": 712111, "stop": 713820, "strand": "-", "frame": 3, "gene": null, "product": "putative MG397-like protein", "db_xrefs": [ "GO:0016020", "RefSeq:WP_014325626.1", "SO:0001217", "UniParc:UPI0001DD643D", "UniRef:UniRef100_A0AAV5N757", "UniRef:UniRef50_P47637", "UniRef:UniRef90_Q50333" ], "nt": "ATGGTTGTCATTGCTGCTTTATTGGGATCGTTAGCTGTCTTAGCTTTTCTGTTTTACTTGTGGTATTTAACGATTTTCATTATTGTGCATAAAAACCAGCAGTACTTGGAACAGACCAAGGCGCTGTACAACAAGTTAAAACTCAGTAATTTCAACTCGGTAATTGTGCCGTTTCAACTGTTGCGCAGTGAAAAGAAAGCGCTGGAACAACCAGTGAAATTGCTCAAAAGTTTTCAAGAGAGCTTTAACAGCGAAATCAATTCCGCTTTAGAACAGCTGTTAGTTTTTAGTGAACCAAAAGCGCTTTACAACTGCTTTTGGTTTAACCGTAATATTAAGTTGTTGCGCAAAAACTTACAAGGATTACATGACAAGCAACAGCGTTATGTTAAGCTGACTAAAAACGCAATTGCGTACTTTGACAGTAGTTATGATACCCTAGTATTCTACCGACAAGCCTTTTGTTTACTAGTTAGTTTTATTAATGACTTTTTGGTGCATAAGTACGATTCCTTGTTTTACCTCAACATTATTAACCGCATTTCCTTATTGTTTAAGGATGTGGAACTACACATCAAAAATAAGGATGTGAAACAGCAAAACCAAGCGTTGAATAAACTCCACAATAACTTAGCACAAACCATTATTACCGCATCAAGACAATACTTCTTTGATGTCAAAGTAGGCTATTTGAACTACCACTTCCAAATTTTGAGTCAAAAGGTAACGCAATTGCGCTCGATGAAAAACAAAGCAATTAACTCCCAAGAGGTAGCTAAGTTTCAGGAATTAATTACTAATATTGGTGCTACTTTGGCGGAATGTAGCCAAGCGTTGGGCAACATTAACTTAGCGTTGTGTGAACATCGCATTAACCAAGCTAAGGAACAGATTGATGTGTTATCTAATGCTGTTAGTGTTAAAGAAAAAGCGATTAGCTTAGTGGTATCTAATGTGGATAGCTTCTTAAAGTCAATTAACCACTACCAACAACAAAACGCCTTGTTACAGACTTTAATGAAGGAAATTGAGTTAATGTTTCAAAACAACTTGGATACCGTTAACATCATTAACCAGATTAACGAACACTCGCTGTTAATTGCACAAAAAACCCAACTACTCAACCAGGAAAATGCGCTGACCGAATTCATTGATTATGAGAAGTTGTTTCGCTTAATGAGTGAAGCGATGAAGTTATTGGATGGATTGAAAAATCTGTTAAACGAGCTGTTTGCGCTGTCAGCTAATAAGTTTGATGATTACCGCTTTTTTGTCTATACCTTAGATGACTTTCGTTTTAAGTTTTTCCAAATTGAAAACTTAATTGCGAATGAAGAGTTAATTATTTCCGAAAAGACTTTAAAGATTATTAGTCAAGCTAAGCACCAGTTTGATGATATTTTTACCCAAGCGAAAAATGACTATGAGGGTGCTTTTAGCTATTTAAACGAAAAGGTGAGGTTCTTCCAAAACCAACTAACCCATGTGATTGTTGATGTAGTAGGTTTAATGAACCTGAGATCAATGTGTAAACATACCTTTATCTTTGCCAATAAGTACCGCCAAGAAAGCCCCCAAATTAACGAAAGCTTGGAAACGTTGACCCAGTTTTACCAACAGCAGAATTATTCGGAAACTTTGAGTGGTTTAATTAGTTTGTTGGGTAAAATTAAATCATCTGCTAAACAACACCACCTTGATTTAAACTAA", "aa": "MVVIAALLGSLAVLAFLFYLWYLTIFIIVHKNQQYLEQTKALYNKLKLSNFNSVIVPFQLLRSEKKALEQPVKLLKSFQESFNSEINSALEQLLVFSEPKALYNCFWFNRNIKLLRKNLQGLHDKQQRYVKLTKNAIAYFDSSYDTLVFYRQAFCLLVSFINDFLVHKYDSLFYLNIINRISLLFKDVELHIKNKDVKQQNQALNKLHNNLAQTIITASRQYFFDVKVGYLNYHFQILSQKVTQLRSMKNKAINSQEVAKFQELITNIGATLAECSQALGNINLALCEHRINQAKEQIDVLSNAVSVKEKAISLVVSNVDSFLKSINHYQQQNALLQTLMKEIELMFQNNLDTVNIINQINEHSLLIAQKTQLLNQENALTEFIDYEKLFRLMSEAMKLLDGLKNLLNELFALSANKFDDYRFFVYTLDDFRFKFFQIENLIANEELIISEKTLKIISQAKHQFDDIFTQAKNDYEGAFSYLNEKVRFFQNQLTHVIVDVVGLMNLRSMCKHTFIFANKYRQESPQINESLETLTQFYQQQNYSETLSGLISLLGKIKSSAKQHHLDLN", "aa_hexdigest": "cd32e1f5e6c683bf536750be336d0065", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD643D", "ncbi_nrp_id": "WP_014325626.1", "uniref100_id": "UniRef100_A0AAV5N757", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD643D", "RefSeq:WP_014325626.1", "UniRef:UniRef100_A0AAV5N757" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N757", "uniref90_id": "UniRef90_Q50333" }, "psc": { "uniref90_id": "UniRef90_Q50333", "product": "Uncharacterized protein MG397 homolog", "uniref50_id": "UniRef50_P47637", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47637", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47637" ], "product": "Uncharacterized protein MG397" }, "genes": [], "id": "CNOCLNGIAL_1260", "locus": "GCA000143945_01260" }, { "type": "cds", "sequence": "contig_1", "start": 713820, "stop": 714221, "strand": "-", "frame": 1, "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "db_xrefs": [ "COG:C", "COG:COG0355", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0045261", "GO:0046933", "RefSeq:WP_010874954.1", "SO:0001217", "UniParc:UPI00001264FB", "UniRef:UniRef100_Q50332", "UniRef:UniRef50_P47638", "UniRef:UniRef90_Q50332" ], "nt": "ATGCAACCATTGCGCTTTTTAGTCTTAAGTCCCAGTGGTATTAAACTCGATCAAGCCATTATTAGTGCGCAAGTCAAAACCACTAATGGTTATTTGGGTTTGAACTATAACCGTGCGCCCTTAATTGCGGCCGTACAATCGCACTTTTGCAAAATTCTGTTTGCCAATAACACGCGGCGCAATGCCATCATAGGAGCGGGGTTAGTGCTCATTAAGAAAACTGAGGCCAAGATCTTTACCGAGAACTTTGTCTTTGAGGATGAAATAGATCTGGAAGCGACTTTGAAGCGTAAGGCCGAGTTAGAGCGGCAAATGAACCACTCGAAGGACTTAAAGCTCAACATTAAGATTGAACAAAACTTAATGTTTGAACTGTTAAAGCTTTCCAATAAGCAACGCTAA", "aa": "MQPLRFLVLSPSGIKLDQAIISAQVKTTNGYLGLNYNRAPLIAAVQSHFCKILFANNTRRNAIIGAGLVLIKKTEAKIFTENFVFEDEIDLEATLKRKAELERQMNHSKDLKLNIKIEQNLMFELLKLSNKQR", "aa_hexdigest": "cf26a0ac06b3935c1b93e9248ddb46c2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001264FB", "ncbi_nrp_id": "WP_010874954.1", "uniref100_id": "UniRef100_Q50332", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001264FB", "RefSeq:WP_010874954.1", "UniRef:UniRef100_Q50332" ] }, "ips": { "uniref100_id": "UniRef100_Q50332", "uniref90_id": "UniRef90_Q50332" }, "psc": { "uniref90_id": "UniRef90_Q50332", "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47638", "cog_id": "COG0355", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045261", "GO:0005524", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47638", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47638" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "CNOCLNGIAL_1261", "locus": "GCA000143945_01261" }, { "type": "cds", "sequence": "contig_1", "start": 714224, "stop": 715651, "strand": "-", "frame": 2, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961", "RefSeq:WP_010874955.1", "SO:0001217", "UniParc:UPI0000126461", "UniRef:UniRef100_Q50331", "UniRef:UniRef50_P50002", "UniRef:UniRef90_Q50331" ], "nt": "ATGAAAAAGGAAAACATTACATACGGTAAGGTCCACCAAGTGATTGGCCCGGTAGTTGATGTCATCTTTACGGAAAGTAGTCAGTTACCCCGCATTTACGATTGTTTGAGTGTTAAGTTAGCTGGGGAAGAACTGTTTTTGGAAGCCGCGCAGCTAATAGGCGATGACATTGTGCGCTGCATTGCTTTGGGTCCAACTGAAGGGTTAGCACGCAACGAAAAGGTGACTAACTATAACCACCCGATTGAAGTCCCAGTTGGCAAAAACGTCTTGGGCCGGATGTTTAATGTTTTGGGTAAACCCATTGACGGGAAGGAGGAGTTACCCAAAAAACCACAACTCCCGATTCACCGCAAACCACCTTCGTTTGATGACCAGTCCAATACGCTAGAAATCTTTGAAACAGGGATTAAGGTGATTGACTTGCTCACTCCCTATGCCCGTGGTGGTAAGATTGGTTTATTTGGTGGTGCTGGTGTTGGTAAAACGGTTTTAGTGCAAGAGTTAATCCACAACATTGCTAAGGAACACTCTGGTTTAAGTGTGTTTGCTGGTGTGGGGGAACGCACCCGGGAAGGTAATGATCTCTACTATGAAATGATCCAGGGTGGGGTGATTGACAAAACTGCTTTAGTGTTTGGTCAGATGAACGAACCCCCAGGAGCACGGATGCGGGTCGCTTTAACGGCGCTTACTATGGCCGAATACTTCCGTGACCATGACAACCAGGATGTCTTGCTCTTCATTGACAACATCTTCCGTTTTACCCAAGCTGGTAGTGAGGTATCAGCACTGTTAGGTCGGATGCCATCAGCCGTGGGTTACCAACCAACGTTGGCGACTGAAATGGGGCAATTACAAGAGCGGATTGCTTCCACTAAAACCGGTTCGATTACCTCGGTGCAAGCGATCTATGTGCCAGCTGATGACTTGACTGACCCCGCTCCGGCTACCACCTTTACCCACCTGGATGCTAAAACGGTGTTAGACCGGAACATAGCAGCGTTGGGAATTTTTCCAGCGATCAATCCCTTAGAGTCTACCAGTCGCTTATTGGATCCTAATATTGTCGGGATTAACCACTATAAGGTAGCGTTAGGGGTGCAAAACATCTTACAGCGCTTTGCGGAACTACAAGACATTATTGCCATCTTGGGAATTGATGAGTTAGCGGATGAGGACAAGATCATTGTGGAACGGGCACGCCGGATCCGCAACTTCCTCTCCCAACCGTTCTTTGTAGCGGAAAAGTTCTCGGGGATTGCTGGGAAGTATGTCCCTTTAAGTGACACGATCCAATCGTTTAAGGAAATCTTGGACGGTAAGCATGATGATCTCCCCGAACAGGCCTTCTTCTTTGTTGGTACCATTCAGGAAGCAGTGGAGAAAGCGAAGCGGCTTAAAAAAGCTACTGTTGAGGAGAAATAA", "aa": "MKKENITYGKVHQVIGPVVDVIFTESSQLPRIYDCLSVKLAGEELFLEAAQLIGDDIVRCIALGPTEGLARNEKVTNYNHPIEVPVGKNVLGRMFNVLGKPIDGKEELPKKPQLPIHRKPPSFDDQSNTLEIFETGIKVIDLLTPYARGGKIGLFGGAGVGKTVLVQELIHNIAKEHSGLSVFAGVGERTREGNDLYYEMIQGGVIDKTALVFGQMNEPPGARMRVALTALTMAEYFRDHDNQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERIASTKTGSITSVQAIYVPADDLTDPAPATTFTHLDAKTVLDRNIAALGIFPAINPLESTSRLLDPNIVGINHYKVALGVQNILQRFAELQDIIAILGIDELADEDKIIVERARRIRNFLSQPFFVAEKFSGIAGKYVPLSDTIQSFKEILDGKHDDLPEQAFFFVGTIQEAVEKAKRLKKATVEEK", "aa_hexdigest": "a5f6f0fc6f01080bf3da9392d68c15c1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000126461", "ncbi_nrp_id": "WP_010874955.1", "uniref100_id": "UniRef100_Q50331", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000126461", "RefSeq:WP_010874955.1", "UniRef:UniRef100_Q50331" ] }, "ips": { "uniref100_id": "UniRef100_Q50331", "uniref90_id": "UniRef90_Q50331" }, "psc": { "uniref90_id": "UniRef90_Q50331", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P50002", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P50002", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P50002" ], "product": "ATP synthase subunit beta, sodium ion specific" }, "genes": [ "atpD" ], "id": "CNOCLNGIAL_1262", "locus": "GCA000143945_01262" }, { "type": "cds", "sequence": "contig_1", "start": 715651, "stop": 716085, "strand": "-", "frame": 3, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_Q50330" ], "nt": "TTGAGCTTTGATTACTGTCAACAGGTGTCCGACCAAATTATGGAGCAGTTTGATTTACACCAATTAGACCGTATTTGCATCATTTACACCCAGTTTAAAAACCCCTTAATTCAACACGCCAACAGCTTCCAGGTTTTTCCCTTTGATGTAGCGATGTTTAAGGCCTTCAACCCGGTCAAAATGGAACAAAAGCTCGACTTTGAACCCGATGAGGAGACAATTATTAAGCTCATTAGTCCCCAGTTCTTTGACATTGCGTTATACGGTGGGTTGGTAGAGACAAAGTTGTGTGAGTCAGCATCAAGACAAAACGCCATGGATGCCGCTGCTAAGAATGCCAAGGATCTGTATGAAAAGTACAGTATTCAATTTAATAAGTTAAGACAAAACTCTATTACCCAAGAAATTATTGAAATCATAGGAGGTATTAGTTAA", "aa": "MSFDYCQQVSDQIMEQFDLHQLDRICIIYTQFKNPLIQHANSFQVFPFDVAMFKAFNPVKMEQKLDFEPDEETIIKLISPQFFDIALYGGLVETKLCESASRQNAMDAAAKNAKDLYEKYSIQFNKLRQNSITQEIIEIIGGIS", "aa_hexdigest": "118938d06364c706d2bc1fb92b85c8bf", "start_type": "TTG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "3-prime", "pseudogene": { "start": 715651, "stop": 716385, "observations": { "mutation": [ 716145 ], "stop": [ 716145 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50330", "query_cov": 1.0, "subject_cov": 0.5161290322580645, "identity": 0.993, "score": 286.0, "evalue": 9.69e-96, "gene_start": 1, "gene_end": 144, "reference_start": 136, "reference_end": 279, "reference_sequence": "MAFIQEIKRKMTTVQSTIKITNAMKMVSRAKFVRFKKQFKEVNYFFNEFYKAVGQVVLSLKKMPKPLENPKTLWVMMSSSLGLCGQHNTNMNKLLQTKFQPGDKIFFLGRKNQSFWNKGDTDNPTVGYVDIQDRDLSFDYCQQVSDQIMEQFDLHQLDRICIIYTQFKNPLIQHANSFQVFPFDVAMFKAFNPVKMEQKLDFEPDEETIIKLISPQFFDIALYGGLVETKLCESASRQNAMDAAAKNAKDLYEKYSIQFNKLRQNSITQEIIEIIGGIS" }, "paralog": false, "identity": 0.9959016393442623, "score": 492.0, "evalue": 2.58e-179, "description": "Internal stop codon at 716145. Nonsense mutation around 716145.", "psc": { "uniref90_id": "UniRef90_Q50330", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50330", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "CNOCLNGIAL_1263", "locus": "GCA000143945_01263" }, { "type": "cds", "sequence": "contig_1", "start": 716143, "stop": 716490, "strand": "-", "frame": 3, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_Q50330" ], "nt": "ATGGCCTTTATCCAGGAAATTAAGCGCAAAATGACCACGGTGCAGTCCACCATTAAGATTACTAACGCAATGAAAATGGTGTCCCGTGCTAAGTTTGTCCGCTTTAAAAAACAGTTTAAGGAAGTTAACTACTTCTTCAATGAGTTTTATAAGGCCGTTGGGCAAGTAGTTCTCAGCTTAAAGAAGATGCCCAAGCCCCTGGAAAATCCCAAAACCCTGTGGGTGATGATGAGTTCATCGTTGGGTCTTTGTGGACAGCACAACACTAACATGAACAAGTTGTTGCAAACGAAGTTCCAACCCGGTGACAAAATCTTCTTTTTGGGGCGCAAAAACCAGTCTTTTTGA", "aa": "MAFIQEIKRKMTTVQSTIKITNAMKMVSRAKFVRFKKQFKEVNYFFNEFYKAVGQVVLSLKKMPKPLENPKTLWVMMSSSLGLCGQHNTNMNKLLQTKFQPGDKIFFLGRKNQSF", "aa_hexdigest": "d84c18f22f3a98013e8fd9bae2cc8332", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "5-prime", "pseudogene": { "start": 715840, "stop": 716490, "observations": { "mutation": [ 716145 ], "stop": [ 716145 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50330", "query_cov": 1.0, "subject_cov": 0.4121863799283154, "identity": 1.0, "score": 228.0, "evalue": 1.27e-73, "gene_start": 1, "gene_end": 115, "reference_start": 1, "reference_end": 115, "reference_sequence": "MAFIQEIKRKMTTVQSTIKITNAMKMVSRAKFVRFKKQFKEVNYFFNEFYKAVGQVVLSLKKMPKPLENPKTLWVMMSSSLGLCGQHNTNMNKLLQTKFQPGDKIFFLGRKNQSFWNKGDTDNPTVGYVDIQDRDLSFDYCQQVSDQIMEQFDLHQLDRICIIYTQFKNPLIQHANSFQVFPFDVAMFKAFNPVKMEQKLDFEPDEETIIKLISPQFFDIALYGGLVETKLCESASRQNAMDAAAKNAKDLYEKYSIQFNKLRQNSITQEIIEIIGGIS" }, "paralog": false, "identity": 0.9953703703703703, "score": 436.0, "evalue": 1.41e-157, "description": "Internal stop codon at 716145. Nonsense mutation around 716145.", "psc": { "uniref90_id": "UniRef90_Q50330", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50330", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "CNOCLNGIAL_1264", "locus": "GCA000143945_01264" }, { "type": "cds", "sequence": "contig_1", "start": 716490, "stop": 718046, "strand": "-", "frame": 1, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "db_xrefs": [ "COG:C", "COG:COG0056", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961", "RefSeq:WP_014325628.1", "SO:0001217", "UniParc:UPI0001DD644D", "UniRef:UniRef100_A0AB38W5W8", "UniRef:UniRef50_P33252", "UniRef:UniRef90_Q50329" ], "nt": "ATGGCAGACAAATTAAATGAATATGTAGCACTCATTAAAAATGAGATTAAGAAGTATTCCAAACAGATCTTTAACAGTGAAATTGGCAAGGTTATTAGTGTCGCTGATGGCATTGCTAAGGTCAGTGGTTTGGAAAACGCACTCTTAAACGAGTTAATTGAGTTTGAAAACAACGTGCAGGGGATTGCGCTTAACCTAGAGCAAAACACGGTTGGGGTGGCGTTGTTTGGTGATTACTCTAAAATCCGTGAAGGTAGTACGGCTAAACGGACCCACAACGTGATGCAAACTCCCGTGGGTGATGTAATGTTGGGCCGGATTGTCAATGCCCTGGGAGAACCGGTGGATGGTCGTGGTCCGATTAAGGCTGAAGAGTTTGACCAAGTGGAGAAGATTGCCCCTGGGGTAATGACCCGCAAAACCGTTAACCAACCACTGGAAACTGGAATCTTGACGATTGATGCGTTGTTTCCGATTGGTAAGGGACAACGGGAGTTAATTGTGGGGGACCGCCAAACTGGTAAGACCTCAATTGCAATTGACACCATTATTAACCAACGTGGTAAGGACGTGTACTGTGTCTATGTAGCGATGGGACAAAAGAATTCCTCGGTAGCGCAAATTGTCCACCAGTTAGAGGTAACTGATTCGATGAAGTACACCACGGTGGTGTGTGCTACAGCCAGTAATCCCGCTTCGATGATCTACCTCACGCCCTTTACTGGCATTACCATTGCTGAGTACTGGCTCAAACAGGGTAAGGATGTTCTAATTGTGTTTGATGATTTGTCCAAGCACGCGATTGCTTACCGTACCTTATCCTTACTGTTACGCCGACCACCAGGACGGGAAGCCTTTCCTGGGGATGTGTTCTACTTGCACTCGCGTTTACTGGAACGTGCTTGCCGTTTAAACGAGGAGCATGGTGGTGGCTCGATTACCGCTTTACCTATTATTGAAACGCAAGCTGGGGACATTTCCGCTTACATTCCGACCAACGTCATTTCGATTACCGATGGTCAACTGTTCATGGTGTCAAACCTGTTTAACTCCGGTCAACGCCCTGCCATCCACGTGGGGTTATCGGTGTCGCGGGTTGGTAGTGCAGCGCAAATTAAAGCAATTAAACAACAAACCGGGAGCTTAAAGTTAGAGCTAGCGCAGTACAGTGAATTAGACAGCTTTAGTCAGTTTGGTAGTGACTTGGACGAAAATACCAAGCAAATCTTGGAACGCGGTAAGCGGGTAATGGAGATGATTAAACAACCCAACGGTAAACCTTACTCACAAACCCATGAAGCGTTATTCTTGTTTGCGATTGCCAAGTCCTTCATTAAGTTTATTCCACTGGATTACATTGCTAAATTTAAGCAACGCATTATGGAGGAATTTGACAAGGAACACCCGATTTACAAGGAAATAGCGACCCAAAAGAGCTTTAGTGAAGCGTTGGAAGCACAAACTAACACTGCTTTTAAGGCACTAGTAAAACGCTTTGTTAGTGCTTTACCAGATTACGACATCACTAAATACGGAACGATGGAGGAGTTGGAATAA", "aa": "MADKLNEYVALIKNEIKKYSKQIFNSEIGKVISVADGIAKVSGLENALLNELIEFENNVQGIALNLEQNTVGVALFGDYSKIREGSTAKRTHNVMQTPVGDVMLGRIVNALGEPVDGRGPIKAEEFDQVEKIAPGVMTRKTVNQPLETGILTIDALFPIGKGQRELIVGDRQTGKTSIAIDTIINQRGKDVYCVYVAMGQKNSSVAQIVHQLEVTDSMKYTTVVCATASNPASMIYLTPFTGITIAEYWLKQGKDVLIVFDDLSKHAIAYRTLSLLLRRPPGREAFPGDVFYLHSRLLERACRLNEEHGGGSITALPIIETQAGDISAYIPTNVISITDGQLFMVSNLFNSGQRPAIHVGLSVSRVGSAAQIKAIKQQTGSLKLELAQYSELDSFSQFGSDLDENTKQILERGKRVMEMIKQPNGKPYSQTHEALFLFAIAKSFIKFIPLDYIAKFKQRIMEEFDKEHPIYKEIATQKSFSEALEAQTNTAFKALVKRFVSALPDYDITKYGTMEELE", "aa_hexdigest": "aa7c409612fcd9c1f0b44434d938fe62", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD644D", "ncbi_nrp_id": "WP_014325628.1", "uniref100_id": "UniRef100_A0AB38W5W8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD644D", "RefSeq:WP_014325628.1", "UniRef:UniRef100_A0AB38W5W8" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W5W8", "uniref90_id": "UniRef90_Q50329" }, "psc": { "uniref90_id": "UniRef90_Q50329", "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P33252", "cog_id": "COG0056", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P33252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P33252" ], "product": "ATP synthase subunit alpha" }, "genes": [ "atpA" ], "id": "CNOCLNGIAL_1265", "locus": "GCA000143945_01265" }, { "type": "cds", "sequence": "contig_1", "start": 718049, "stop": 718585, "strand": "-", "frame": 2, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_014325629.1", "SO:0001217", "UniParc:UPI0001DD644E", "UniRef:UniRef100_A0AAV5NA96", "UniRef:UniRef50_P47642", "UniRef:UniRef90_Q50328" ], "nt": "ATGATTAACGCGCAGGCTTTTGGAATGGCACTCTTCCAACTTAGTGAAGAGGAAAAGAAGGTTAAGAAGGTCTATGACCAGAGCCATGACTTTTTAAAACTAGCGCGTAACTTTAAAGATGGATCACTAGCTGCTCTACTCAATACTTATACTTTAACCAAAAAGGAAAAGCTTAAGCTAATAGATAAGCTGTTTAAAAACCACTTTTGTGACCTCTTTGTTGACTTTTTAAAAACGATTGTGCTCAAGGGCTACTTTAACCTAGTCGAGCAAGCCTTAAAGTACTTCTTTGATTGTGTTGAGAATGAAAAACACGTCCAGTTCATTAGGATCATTACCGCTTTTGAACTGAGTGCGCCCCAACTCAAGCGAATCGTAGCGGCAATGGAAAAGAAGCTCAATTCCAAGATTGTTTACAAAACCGAAATTGACAAGAGCTTGATTTCGGGTATCCGAATTGAATCGAGCGCCCAGTTATTTGAAAAGAATATCCGTGATCAACTATCACGTTTAATGGAACAGTTTAAAGGGAATTAA", "aa": "MINAQAFGMALFQLSEEEKKVKKVYDQSHDFLKLARNFKDGSLAALLNTYTLTKKEKLKLIDKLFKNHFCDLFVDFLKTIVLKGYFNLVEQALKYFFDCVENEKHVQFIRIITAFELSAPQLKRIVAAMEKKLNSKIVYKTEIDKSLISGIRIESSAQLFEKNIRDQLSRLMEQFKGN", "aa_hexdigest": "13f5afe57158869d964d14340f523d4d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD644E", "ncbi_nrp_id": "WP_014325629.1", "uniref100_id": "UniRef100_A0AAV5NA96", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD644E", "RefSeq:WP_014325629.1", "UniRef:UniRef100_A0AAV5NA96" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NA96", "uniref90_id": "UniRef90_Q50328" }, "psc": { "uniref90_id": "UniRef90_Q50328", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47642", "cog_id": "COG0712", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47642", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47642" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "CNOCLNGIAL_1266", "locus": "GCA000143945_01266" }, { "type": "cds", "sequence": "contig_1", "start": 718578, "stop": 718952, "strand": "-", "frame": 1, "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "db_xrefs": [ "COG:C", "COG:COG0711", "EC:7.1.2.2", "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961", "SO:0001217", "UniRef:UniRef50_Q50327", "UniRef:UniRef90_Q50327" ], "nt": "TTGGAAGAGCAAGTCAACCAGGCCAACAGCTTAGAACAACAAGCTCAAGCATTACTCCAACAAGCCAACCAACGCCATGAAAACTCACTGGTAGTTGCTAAGGAAATTGTAGACCAGGCCAACTATGAGGCGTTGCAACTTAAAAGTGAAATCGAAAAGAAGGCTAACCGGCAAGCTAACTTAATGATCTTCCAAGCGCGTCAGGAAATTGAAAAGGAAAAGCGCTTGATCCAAGAACAATCCTTAAAGGAAAGTGTCGAGCTAGCCATGTTAGCTGCTAAGGAATTGATTATCAAAAAGGTGGATGTCAAAGCCGACAAGGCCTTTATTGAAGAGTTTATCCGCGAACTAGAAGCAGAGGACGACCATGATTAA", "aa": "MEEQVNQANSLEQQAQALLQQANQRHENSLVVAKEIVDQANYEALQLKSEIEKKANRQANLMIFQARQEIEKEKRLIQEQSLKESVELAMLAAKELIIKKVDVKADKAFIEEFIRELEAEDDHD", "aa_hexdigest": "0a202e2ebfc50ed763cad45145aeba80", "start_type": "TTG", "rbs_motif": null, "genes": [ "atpF" ], "truncated": "3-prime", "pseudogene": { "start": 718578, "stop": 719201, "observations": { "mutation": [ 718994 ], "stop": [ 718994 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50327", "query_cov": 1.0, "subject_cov": 0.5990338164251208, "identity": 0.992, "score": 210.0, "evalue": 3.69e-67, "gene_start": 1, "gene_end": 124, "reference_start": 84, "reference_end": 207, "reference_sequence": "MKLRATFVFKTTLVALSFALFALFLVSCTENVKEIKSESVINELFPNLWVFLAHLLAFVILLFLLLFLFWKPTQKFLNQRKALLEEQVNQANSLEQQAQALLQQANQRHENSLVVAKEIVDQANYEALQLKSEIEKKANRQANLMIFQARQEIEKEKRLIQEQSLKESVELAMLAAKELIIKKVDVKADKAFIEEFIRELEAEDDHD" }, "paralog": false, "identity": 0.9951690821256038, "score": 386.0, "evalue": 4.54e-139, "description": "Internal stop codon at 718994. Nonsense mutation around 718994.", "psc": { "uniref90_id": "UniRef90_Q50327", "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q50327", "cog_id": "COG0711", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50327", "EC:7.1.2.2", "UniRef:UniRef50_Q50327", "COG:COG0711", "COG:C", "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_Q50327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50327" ], "product": "ATP synthase subunit b" } }, "id": "CNOCLNGIAL_1267", "locus": "GCA000143945_01267" }, { "type": "cds", "sequence": "contig_1", "start": 718992, "stop": 719201, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTACGAGCAACTTTTGTATTTAAAACGACCTTAGTTGCGCTTAGTTTTGCCCTGTTTGCCTTGTTTTTAGTTTCCTGTACCGAAAACGTTAAGGAAATTAAAAGTGAATCAGTCATCAACGAACTTTTTCCGAACCTCTGGGTCTTTTTAGCCCACTTGTTGGCCTTTGTGATCCTGTTGTTCTTGTTGTTGTTCCTCTTTTGA", "aa": "MKLRATFVFKTTLVALSFALFALFLVSCTENVKEIKSESVINELFPNLWVFLAHLLAFVILLFLLLFLF", "aa_hexdigest": "5878f6f07991407453cf537e44b74ec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7955.630299999993, "isoelectric_point": 7.940844917297363 }, "id": "CNOCLNGIAL_1268", "locus": "GCA000143945_01268" }, { "type": "cds", "sequence": "contig_1", "start": 719204, "stop": 719521, "strand": "-", "frame": 2, "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0005886", "GO:0008289", "GO:0045263", "GO:0046933", "RefSeq:WP_010874960.1", "SO:0001217", "UniParc:UPI00001265D2", "UniRef:UniRef100_Q59550", "UniRef:UniRef50_P47644", "UniRef:UniRef90_Q59550" ], "nt": "ATGGAACACGTTAATGAAATTCTCGCCACAGTTGGCCGCATCTTACACGAAACAACAACAGCTAATACCAATGTTGCCAATAAAAGTACCGAACGTTTAGGGGCTTACATTGGGGCTGGGATTACCATGGTCGGTGGTGCGACCGTGGGTCTTGGTCAAGGTTACATCTTTGGGAAAGCGGTTGAAGCCGTAGCGCGGAACCCCGAAGTGGAAAAGCAGGTCTTTAAATTAATCTTTATTGGCTCTGCTATTAGTGAATCGAGTTCCATCTATTCCTTGTTAATAGCCTTCATTCTTATCTTTGTATCGGGCGCTTAA", "aa": "MEHVNEILATVGRILHETTTANTNVANKSTERLGAYIGAGITMVGGATVGLGQGYIFGKAVEAVARNPEVEKQVFKLIFIGSAISESSSIYSLLIAFILIFVSGA", "aa_hexdigest": "db463fb393f3e86270e9f374118cb4dc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001265D2", "ncbi_nrp_id": "WP_010874960.1", "uniref100_id": "UniRef100_Q59550", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001265D2", "RefSeq:WP_010874960.1", "UniRef:UniRef100_Q59550" ] }, "ips": { "uniref100_id": "UniRef100_Q59550", "uniref90_id": "UniRef90_Q59550" }, "psc": { "uniref90_id": "UniRef90_Q59550", "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47644", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045263", "GO:0008289", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47644" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "CNOCLNGIAL_1269", "locus": "GCA000143945_01269" }, { "type": "cds", "sequence": "contig_1", "start": 719524, "stop": 719874, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00119.25" ], "nt": "TTGAGCTTCTTGGGTGAGTTTGCGCCACTGTTCTCGATTTCGCTTCGTTTGTGGGGCAATATCCTTGGGGGAACCTTGATTTTAGCGCTCTTTTATAACTTCTGGTTCTTTGCTTTTTCGACTTTAAGCAATAAGCCACTAGCGCTTAGTTTAGGTGCCATTTTTGCAGGAATTCTCACCCCAGCCCTACATGTATATTTTGATGTAGTAGTGGGTACACTGCAGGGATATGTCTTTGTGATGTTGACTTATAATTATTGGGCAAAAATGCGTAACATTGGCTTGGAAGAAAGTCAAGAAGCAGCGCAGCGTTTGCAAAATTTGGAGGTAGCAAAAGAAATAATTAATTAG", "aa": "MSFLGEFAPLFSISLRLWGNILGGTLILALFYNFWFFAFSTLSNKPLALSLGAIFAGILTPALHVYFDVVVGTLQGYVFVMLTYNYWAKMRNIGLEESQEAAQRLQNLEVAKEIIN", "aa_hexdigest": "965d7137397394ff0a697528c60a6335", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00119.25", "name": "ATP-synt_A", "length": 90, "aa_cov": 0.7413793103448276, "hmm_cov": 0.9, "evalue": 5.672747014371094e-19, "score": 64.29066467285156, "start": 2, "stop": 87 } ], "seq_stats": { "molecular_weight": 13047.127499999991, "isoelectric_point": 5.668689155578614 }, "id": "CNOCLNGIAL_1270", "locus": "GCA000143945_01270" }, { "type": "cds", "sequence": "contig_1", "start": 719891, "stop": 720103, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGTACTTCTTGCCCTTAATCGTAATGTTGAAGGTAAAGCTTTTAAAGAAGGATCACCGTTGGTACCTAATCCCCATAATGACAATTCCCAAAAAGGTGGCTAGCCCTAAAGAAAAGGTAAAGGTCAGTGATGAAGTTGGTGGGGAAATGCCACCTAATAATCCAATCAAGTTACTGGAAACCAGGTACAACAGCAACATTAAAAAGTAA", "aa": "MEYFLPLIVMLKVKLLKKDHRWYLIPIMTIPKKVASPKEKVKVSDEVGGEMPPNNPIKLLETRYNSNIKK", "aa_hexdigest": "9e2672b52be875872eced49919f45324", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8192.855099999997, "isoelectric_point": 9.822548103332519 }, "id": "CNOCLNGIAL_1271", "locus": "GCA000143945_01271" }, { "type": "cds", "sequence": "contig_1", "start": 720886, "stop": 722220, "strand": "-", "frame": 3, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "COG:COG0148", "COG:G", "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0005829", "GO:0006096", "GO:0009986", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P75189", "UniRef:UniRef90_P75189" ], "nt": "ATGAGTGCACAAACTGGAACCGATCTTTTCAAGATTGCTGATCTTTTTGCTTACCAAGTTTTTGATTCACGGGGTTTTCCCACTGTTGCTTGTGTTGTGAAGTTAGCTAGTGGTCATACTGGAGAAGCGATGGTTCCATCTGGGGCTTCTACTGGGGAAAAAGAAGCGATTGAACTACGCGATGGTGATCCCAAAGCTTACTTTGGCAAAGGGGTGAGCCAAGCTGTGCAAAACGTTAACCAAACTATTGCCCCTAAACTAATTGGTTTAAATGCGACTGATCAAGCAGCCATTGATGCTTTAATGATCCAACTGGATGGTACCCCCAATAAGGCTAAGTTAGGTGCTAATGCCATTTTGGCAGTTTCTCTAGCTGTGGCTAAAGCAGCTGCTAGCGCCCAAAAGACTTCCTTGTTTAAGTACCTTGCTAACCAGGTCATGGGTCTAAATAAAACCGAGTTTATCCTCACCGTACCAATGCTAAACGTCATTAACGGTGGAGCACATGCTGACAATAACATTGACTTCCAGGAGTTCATGATTATGCCATTGGGTGCTAACTCGATGCACCAAGCATTGAAGATGGCGAGTGAAACCTTCCATGCTTTACAAAAGCTGTTAAAGCAACGGGGTTTAAACACCAATAAGGGTGATGAAGGTGGTTTTGCGCCTAACTTAAAGTTAGCGGAAGAAGCGCTTGATTTAATGGTCGAAGCAATTAAGGCAGCTGGGTACCAACCAGGTAGTGACATTGCGATTGCACTGGATGTAGCAGCGAGTGAGTTTTACGATGACACCACCAAGCGCTATGTCTTCAAAAAAGGAATTAAGGCCAAAATTCTTGACGAGAAGGAATGGAGTTTAACTACTGCCCAAATGATCGCTTACCTCAAGAAGCTTACTGAGCAGTACCCTATTATTTCCATAGAAGATGGTCTTAGCGAACACGACTGGGAAGGTATGGAAACATTAACTAAAACACTGGGTCAACACATTCAAATAGTTGGTGATGATCTGTACTGCACCAATCCAGCAATTGCTGAAAAAGGTGTTGCTCACAAAGCTACCAACTCGATCTTAATTAAACTCAACCAAATTGGTACCCTTACAGAAACAATTAAAGCGATTAACATTGCCAAGGACGCTAACTGGAGTCAGGTAATTTCCCACCGTAGTGGGGAAACCGAAGACACGACAATTGCTGATCTAGCAGTAGCGGCTTGCACTGGTCAAATTAAAACTGGTTCGATGTCCCGCTCCGAACGAATTGCCAAATACAATCGCTTATTGCAAATTGAACTGGAATTGGGCAATAATGCTAAGTACTTAGGTTGA", "aa": "MSAQTGTDLFKIADLFAYQVFDSRGFPTVACVVKLASGHTGEAMVPSGASTGEKEAIELRDGDPKAYFGKGVSQAVQNVNQTIAPKLIGLNATDQAAIDALMIQLDGTPNKAKLGANAILAVSLAVAKAAASAQKTSLFKYLANQVMGLNKTEFILTVPMLNVINGGAHADNNIDFQEFMIMPLGANSMHQALKMASETFHALQKLLKQRGLNTNKGDEGGFAPNLKLAEEALDLMVEAIKAAGYQPGSDIAIALDVAASEFYDDTTKRYVFKKGIKAKILDEKEWSLTTAQMIAYLKKLTEQYPIISIEDGLSEHDWEGMETLTKTLGQHIQIVGDDLYCTNPAIAEKGVAHKATNSILIKLNQIGTLTETIKAINIAKDANWSQVISHRSGETEDTTIADLAVAACTGQIKTGSMSRSERIAKYNRLLQIELELGNNAKYLG", "aa_hexdigest": "bafd65c3ff0d85920093de9bf30d0326", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75189", "query_cov": 1.0, "subject_cov": 0.9568965517241379, "identity": 1.0, "score": 859.0, "evalue": 2.48e-314, "valid": true, "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P75189", "cog_id": "COG0148", "cog_category": "G", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0005829", "GO:0006096", "GO:0009986" ] }, "pscc": { "uniref50_id": "UniRef50_P75189", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75189" ], "product": "Enolase" }, "genes": [ "eno" ], "id": "CNOCLNGIAL_1272", "locus": "GCA000143945_01272" }, { "type": "cds", "sequence": "contig_1", "start": 722275, "stop": 722748, "strand": "+", "frame": 1, "gene": "msrA", "product": "Peptide methionine sulfoxide reductase MsrA", "db_xrefs": [ "COG:COG0225", "COG:O", "EC:1.8.4.11", "GO:0005737", "GO:0008113", "GO:0034599", "GO:0036211", "GO:0036456", "RefSeq:WP_010874964.1", "SO:0001217", "UniParc:UPI000012F657", "UniRef:UniRef100_P75188", "UniRef:UniRef50_P75188", "UniRef:UniRef90_P75188" ], "nt": "ATGAAACAAATCTATTTTGGTGGTGGCTGTTTTTGGGGTACCCAAAAGTATTTTGATCTGATTAAGGGTGTCCAAAAGACCAGTGTGGGCTACCTCAATTCCAACATGAAAAATCCCACTTACGAACAAGTATGTAGTGGGCAGACCAACGCAGTGGAAGCAGTGTTCGTTGAATATGATGAAAACAAGGTAAGCTTAAACGAACTTATTGATGCTTTCTTTAAGGTCATTGATCCCACCATTAGAAACCGTCAGGGTAATGATATTGGTACCCAGTACCGTACCGGAGTTTATTGGGTAGATCCCCAAGACGAGCAGTTAATTACGCAAAAGTTTAGGGAACTACAGGCCAATTATCCAAAACCAATTGTGACGGAAAACCGTGCAATGGAAAACTATTTTTTAGCAGAAGAGTACCACCAGGATTACTTAAAGAAAAATCCTCATGGTTACTGTCACATTAAGTTTGACTAG", "aa": "MKQIYFGGGCFWGTQKYFDLIKGVQKTSVGYLNSNMKNPTYEQVCSGQTNAVEAVFVEYDENKVSLNELIDAFFKVIDPTIRNRQGNDIGTQYRTGVYWVDPQDEQLITQKFRELQANYPKPIVTENRAMENYFLAEEYHQDYLKKNPHGYCHIKFD", "aa_hexdigest": "a6f32d55a9980d1a2810341e441f7a7f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012F657", "ncbi_nrp_id": "WP_010874964.1", "uniref100_id": "UniRef100_P75188", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012F657", "RefSeq:WP_010874964.1", "UniRef:UniRef100_P75188" ] }, "ips": { "uniref100_id": "UniRef100_P75188", "uniref90_id": "UniRef90_P75188" }, "psc": { "uniref90_id": "UniRef90_P75188", "gene": "msrA", "product": "Peptide methionine sulfoxide reductase MsrA", "ec_ids": [ "1.8.4.11" ], "uniref50_id": "UniRef50_P75188", "cog_id": "COG0225", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0036456", "GO:0008113", "GO:0034599", "GO:0036211" ] }, "pscc": { "uniref50_id": "UniRef50_P75188", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75188" ], "product": "Peptide methionine sulfoxide reductase MsrA" }, "genes": [ "msrA" ], "id": "CNOCLNGIAL_1273", "locus": "GCA000143945_01273" }, { "type": "cds", "sequence": "contig_1", "start": 722745, "stop": 723146, "strand": "-", "frame": 1, "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "db_xrefs": [ "COG:COG0704", "COG:P", "GO:0006817", "GO:0030643", "GO:0045936", "SO:0001217", "UniRef:UniRef50_P47649", "UniRef:UniRef90_P75187" ], "nt": "ATGTGTAGTCGTGATTTGGAACGCATTTGTGATTATGCCAATAACCTCACTAAGTTTGTCAAGCACTACCAGCACTTGGATGTCAGCATCTTTAGTAAGTTAGTTAATTTGCACAAAAGTGTGTTGAACAACTTAAAGCAAACGTTTGCCAGTTTACAAGATAAGGAAAAACCGCTTACTATTCAGTTTGAGAATGTCACTAAAATTCTGACAGAGTTTGAACAGCAGTACCGCGTCGTGTTAACTGAATACTATGACAAGGTTAAAGACGAGAAGCTGAGTGATCGCATCTTCTTAATTGACTTAATTCTGTCGGTGAAACACATTGAACGGATTAATGACTATTGCTACAACATCATTAAGGCATTCCTGTTTGTGAAAAACCCAGAAGTCTTTAACTAG", "aa": "MCSRDLERICDYANNLTKFVKHYQHLDVSIFSKLVNLHKSVLNNLKQTFASLQDKEKPLTIQFENVTKILTEFEQQYRVVLTEYYDKVKDEKLSDRIFLIDLILSVKHIERINDYCYNIIKAFLFVKNPEVFN", "aa_hexdigest": "2ef5fac641a11a628cb67912b411cd79", "start_type": "ATG", "rbs_motif": null, "genes": [ "phoU" ], "truncated": "3-prime", "pseudogene": { "start": 722745, "stop": 723422, "observations": { "mutation": [ 723206 ], "stop": [ 723206 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75187", "query_cov": 1.0, "subject_cov": 0.5911111111111111, "identity": 1.0, "score": 264.0, "evalue": 3.38e-88, "gene_start": 1, "gene_end": 133, "reference_start": 93, "reference_end": 225, "reference_sequence": "MESINYQILKRSEKKLLGLFFDYFQHVIKMHETLNKLLCEADVTKREKLIQAIYEMEDFSNKSEFKLINESIWAISKNSPLTNHLRLTITIIMCSRDLERICDYANNLTKFVKHYQHLDVSIFSKLVNLHKSVLNNLKQTFASLQDKEKPLTIQFENVTKILTEFEQQYRVVLTEYYDKVKDEKLSDRIFLIDLILSVKHIERINDYCYNIIKAFLFVKNPEVFN" }, "paralog": false, "identity": 0.9955555555555555, "score": 443.0, "evalue": 5.15e-161, "description": "Internal stop codon at 723206. Nonsense mutation around 723206.", "psc": { "uniref90_id": "UniRef90_P75187", "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "uniref50_id": "UniRef50_P47649", "cog_id": "COG0704", "cog_category": "P", "go_ids": [ "GO:0006817", "GO:0030643", "GO:0045936" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75187", "UniRef:UniRef50_P47649", "COG:COG0704", "COG:P", "GO:0006817", "GO:0030643", "GO:0045936" ] }, "pscc": { "uniref50_id": "UniRef50_P47649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47649" ], "product": "Uncharacterized protein MG409" } }, "id": "CNOCLNGIAL_1274", "locus": "GCA000143945_01274" }, { "type": "cds", "sequence": "contig_1", "start": 723204, "stop": 723422, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGCATTAACTACCAAATTTTAAAGCGTTCAGAAAAAAAGCTGTTAGGACTCTTTTTTGATTACTTTCAGCACGTCATTAAGATGCATGAAACACTCAATAAACTGTTATGTGAAGCGGATGTAACTAAGCGCGAAAAGTTGATTCAAGCAATTTACGAAATGGAAGACTTTTCCAATAAATCGGAGTTTAAACTAATTAACGAATCCATTTGA", "aa": "MESINYQILKRSEKKLLGLFFDYFQHVIKMHETLNKLLCEADVTKREKLIQAIYEMEDFSNKSEFKLINESI", "aa_hexdigest": "ef26c005b503b2183c85d4866faf7786", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8674.006699999994, "isoelectric_point": 6.086455726623535 }, "id": "CNOCLNGIAL_1275", "locus": "GCA000143945_01275" }, { "type": "cds", "sequence": "contig_1", "start": 723425, "stop": 723973, "strand": "-", "frame": 2, "gene": "pstB", "product": "phosphate ABC transporter ATP-binding protein PstB", "db_xrefs": [ "COG:COG1117", "COG:P", "EC:7.3.2.1", "GO:0005524", "GO:0005886", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0016887", "GO:0035435", "GO:0055052", "SO:0001217", "UniRef:UniRef50_P47650", "UniRef:UniRef90_P75186" ], "nt": "TTGGGTAAAAACATTAATTCCGGAATTATTAATGATTTAACCTTGCGCACACGGGTTGGTATGGTGTTCCAGCAACTCACCCCTTTTAACTTTTCCATCTTTGAAAACATTGCCTATGGACTGAGAGCCCATGGCATTCATAACAAGCAAGCGATTCATGAAATTGTGGAACAGGCCTTAAAGTCAACCGCCTTATGGGACGAGGTCAAAGATAACTTACACCGTAATGCCAATACCCTTTCGGGTGGTCAACAACAACGGTTGTGTATTGCCAGAGCCATTGCTCTACAACCCGATGTCTTATTAATGGATGAACCAACTAGTGCGTTGGACTCAATTGCCACTAACTCGATTGAGCTGTTAATCCAGCAATTAAAGGAAAAATACACGATTATTATTGTGACTCACTCAATGGCACAAACTATTAGAATCACTGATGAAACGATTTTCTTTGCTAACGGAAGGGTAGTGGAACAAGGTACTACCAAGCAAATCTTTACGAGACCAAAGCAAAAGGAAACTAACCGTTACATTAGTGGGAGGAATTAA", "aa": "MGKNINSGIINDLTLRTRVGMVFQQLTPFNFSIFENIAYGLRAHGIHNKQAIHEIVEQALKSTALWDEVKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKYTIIIVTHSMAQTIRITDETIFFANGRVVEQGTTKQIFTRPKQKETNRYISGRN", "aa_hexdigest": "70ff4345f8e9c529a343819ee6637b4f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R5YHY1", "query_cov": 0.9175824175824175, "subject_cov": 0.9881656804733728, "identity": 0.593, "score": 199.0, "evalue": 1.34e-62, "valid": false, "product": "Phosphate ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A836TDX0" }, "genes": [ "pstB" ], "truncated": "3-prime", "pseudogene": { "start": 723425, "stop": 724273, "observations": { "mutation": [ 723994 ], "stop": [ 723994 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75186", "query_cov": 1.0, "subject_cov": 0.5531914893617021, "identity": 0.995, "score": 352.0, "evalue": 1.71e-120, "gene_start": 1, "gene_end": 182, "reference_start": 148, "reference_end": 329, "reference_sequence": "MKKGLKTIWHNFIQKREKVKQYRALYEKQIKQYQQKVAKLDPTTKAEEIANLQSEIDVLQRLIKIKNTKDDVVKQDFDKKNVFEIENLNFWYNKDKQVLFDINLKIKRNKITALIGKSGCGKSTFIRCLNKLNDLNENVRWNGKIFFLGKNINSGIINDLTLRTRVGMVFQQLTPFNFSIFENIAYGLRAHGIHNKQAIHEIVEQALKSTALWDEVKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKYTIIIVTHSMAQTIRITDETIFFANGRVVEQGTTKQIFTRPKQKETNRYISGRN" }, "paralog": false, "identity": 0.9964539007092199, "score": 553.0, "evalue": 4.37e-202, "description": "Internal stop codon at 723994. Nonsense mutation around 723994.", "psc": { "uniref90_id": "UniRef90_P75186", "gene": "pstB", "product": "phosphate ABC transporter ATP-binding protein PstB", "ec_ids": [ "7.3.2.1" ], "uniref50_id": "UniRef50_P47650", "cog_id": "COG1117", "cog_category": "P", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0016887", "GO:0035435", "GO:0055052" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75186", "EC:7.3.2.1", "UniRef:UniRef50_P47650", "COG:COG1117", "COG:P", "GO:0005524", "GO:0005886", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0016887", "GO:0035435", "GO:0055052" ] }, "pscc": { "uniref50_id": "UniRef50_P47650", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47650" ], "product": "Phosphate import ATP-binding protein PstB" } }, "id": "CNOCLNGIAL_1276", "locus": "GCA000143945_01276" }, { "type": "cds", "sequence": "contig_1", "start": 723992, "stop": 724360, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGAAGCAGTACCGTGCTTTATATGAAAAACAAATTAAGCAATACCAGCAAAAAGTTGCTAAGTTAGATCCCACTACCAAAGCTGAGGAAATTGCTAACTTGCAGAGTGAAATTGATGTGTTGCAACGCCTCATCAAAATTAAGAATACCAAGGACGATGTTGTCAAACAAGACTTTGACAAGAAGAATGTCTTTGAAATTGAAAACCTTAACTTCTGGTACAACAAGGACAAGCAGGTCTTATTTGACATTAACCTCAAAATTAAGCGCAATAAGATTACCGCTTTAATTGGTAAATCCGGTTGTGGTAAGTCCACCTTTATCCGTTGTTTAAATAAATTAAATGACCTCAATGAAAATGTGCGCTGA", "aa": "MKQYRALYEKQIKQYQQKVAKLDPTTKAEEIANLQSEIDVLQRLIKIKNTKDDVVKQDFDKKNVFEIENLNFWYNKDKQVLFDINLKIKRNKITALIGKSGCGKSTFIRCLNKLNDLNENVR", "aa_hexdigest": "c3d9d7c7bda39285a93dd15c52942bc5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 34, "aa_cov": 0.2786885245901639, "hmm_cov": 1.0, "evalue": 3.728917650205043e-11, "score": 39.25088882446289, "start": 82, "stop": 115 } ], "seq_stats": { "molecular_weight": 14423.573699999977, "isoelectric_point": 9.562031364440916 }, "id": "CNOCLNGIAL_1277", "locus": "GCA000143945_01277" }, { "type": "cds", "sequence": "contig_1", "start": 724441, "stop": 724974, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCTCGATTATTTACGGATTGTTTGGTCTATCCTTCTTTTTGCGAACACTGCAATTAAGTGCGGGTGGTGCGAATGGTACTAGTTTAATGGCGGGTATTCTTACTATTAGTGTAGTGGTCTTACCCTTTTTAATTCGCACATGTCAGGAAGCACTCAATAATGTCAGTTGGGACTTGCGGGTAAGTGCTTATGCCTTAGGTGTAAGTAAAAGGGAAGTAATTTGGAAAATTGTGTTACCAGGGGCGTTGAAGGGTTTAATTATTGCGCTTATTTTAACGATTAACAGAATTATTGCGGAAACAGCTCCTTTCTTTATTACCGCTGGGTTAGCATCCAGCAACTTGTTTGATTTAAGTCTACCGGGTCAAACCTTAACCACAAGAATTTATGGTCAGTTATTTTCCACTAACAGTACGGCGGTTGATGTAATGCTGGAAACCGCTTTGGTATCAATTGTCTTCCTGATGTTCTTAATCTTTTTAAGCTCTTACTTAATTCCTTACCTGTTTTCGTTTAATAAACAAAAGTGA", "aa": "MPSIIYGLFGLSFFLRTLQLSAGGANGTSLMAGILTISVVVLPFLIRTCQEALNNVSWDLRVSAYALGVSKREVIWKIVLPGALKGLIIALILTINRIIAETAPFFITAGLASSNLFDLSLPGQTLTTRIYGQLFSTNSTAVDVMLETALVSIVFLMFLIFLSSYLIPYLFSFNKQK", "aa_hexdigest": "842b7b2880b4c45e266f286c90844484", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19304.744499999986, "isoelectric_point": 9.461202812194827 }, "id": "CNOCLNGIAL_1278", "locus": "GCA000143945_01278" }, { "type": "cds", "sequence": "contig_1", "start": 725044, "stop": 725220, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGGGATTCTCTTGTTTGTTTTTATTAATGGTAGTCAAGCGATTCAGCGCAGTGGTTCCACTGTCTTTTCGTTTGGTGTAGATACTACAGGGCGAGCTTTAGTCAATACCTTGGTGATTATTCTGGTTGCCATTGGTATTACCTTTCCAATCGCTTTGTTAATTGCCATTTGA", "aa": "MLGILLFVFINGSQAIQRSGSTVFSFGVDTTGRALVNTLVIILVAIGITFPIALLIAI", "aa_hexdigest": "f0a2977fc6b1f3b97b37e326e24a2fff", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6077.2666999999965, "isoelectric_point": 9.3493501663208 }, "id": "CNOCLNGIAL_1279", "locus": "GCA000143945_01279" }, { "type": "cds", "sequence": "contig_1", "start": 725266, "stop": 725424, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGGTTTATTCCTAATAACATTGGCGCTCTTTTTACTGATTTAACTGCTAGAAGACAACAGGTTAAAAAGATTACAGCTGCTAATGTTGAGCAGCGCGCTACCTTCTTCAAGGAAAGGATGCAGACTAACCACTTAAATAAGGTTTACACATCGTGA", "aa": "MWFIPNNIGALFTDLTARRQQVKKITAANVEQRATFFKERMQTNHLNKVYTS", "aa_hexdigest": "241db1da5e0dfd144bc01120f48985f2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6128.9974, "isoelectric_point": 10.533698844909669 }, "id": "CNOCLNGIAL_1280", "locus": "GCA000143945_01280" }, { "type": "cds", "sequence": "contig_1", "start": 725494, "stop": 726354, "strand": "-", "frame": 3, "gene": "pstA", "product": "phosphate ABC transporter permease PstA", "db_xrefs": [ "COG:COG0581", "COG:P", "GO:0005315", "GO:0005886", "GO:0006817", "GO:0015415", "GO:0035435", "GO:0055052", "SO:0001217", "UniRef:UniRef50_P75185", "UniRef:UniRef90_P75185" ], "nt": "GTGAAACAGAAAATTAAAAGCCGTTTAAAAAAAGACAACTGGCTGCGGTACTTATCGCAAACAGTGGCTGTTTGTTTTTTACTGCTCTTCATTAGCTTCTTTATTTTCCTCTTAATTGAAGCAGCTAAAACTGGTCCTGACTTTACCAAGTCCTTACTGGGTTTGGAATTTAACTTGGGTGCTAAAAAAGCGAGCATCTGGTTTCCGCTGTTAGTTAGTTTTGTGGTTTCGATTGGCTCGTTAATTATTGCGAGCTATATCGGTGTTCGCACTTCGATTTTCTTGGTTTATCGTTGCAAACCCCGGATTCGCAAAAAACTACTTTTAGTAATTGACATTCTGTCCGGGATCCCTTCAGTGATCTTTGGATTGTTTGCTACCCAAATTCTCAGCAGTATCTTCCGTGACGTGTTGCACTTGCCACCCTTATCACTGCTTAACGTGATTGTGATGCTGTCCTTCATGATCATTCCGATTGTGATTTCCTTAACCACCAATGCCTTACTGCACGTGGAATCTAGCTTAATGACGGTTGCCATTTCACTTGGTGAAAACAAAACCAGTGTGATTTATAAGGTAATTAAAAAGGAAATCAAAGCCCAACTAGTAGTAATCTTGGTATTAGCGTTTGGTCGTGCCATTAGCGAAACGATGGCTGTGAACTTTATTTTGCAGAGTGTTAACTACCAGGAAGTGATTGCTAATGATCGTTTCTTTACTTCAGATCTCAAAACTTTGGGTTCTGTTATCTCCACCTTTATCTTTTCGGAAAACGGCGATGAACAGGTTAGTGGGGTCTTATACACTTTTGGCATTATTATCTTTGTCTTAATTTCCTTCTTGAACTTTTTTGCCATTTGA", "aa": "MKQKIKSRLKKDNWLRYLSQTVAVCFLLLFISFFIFLLIEAAKTGPDFTKSLLGLEFNLGAKKASIWFPLLVSFVVSIGSLIIASYIGVRTSIFLVYRCKPRIRKKLLLVIDILSGIPSVIFGLFATQILSSIFRDVLHLPPLSLLNVIVMLSFMIIPIVISLTTNALLHVESSLMTVAISLGENKTSVIYKVIKKEIKAQLVVILVLAFGRAISETMAVNFILQSVNYQEVIANDRFFTSDLKTLGSVISTFIFSENGDEQVSGVLYTFGIIIFVLISFLNFFAI", "aa_hexdigest": "98c1f0a0e0b0ea8e6e84632324317ae2", "start_type": "GTG", "rbs_motif": null, "genes": [ "pstA" ], "truncated": "5-prime", "pseudogene": { "start": 725191, "stop": 726354, "observations": { "mutation": [ 725268, 725496 ], "stop": [ 725268, 725496 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75185", "query_cov": 1.0, "subject_cov": 0.4393241167434716, "identity": 1.0, "score": 506.0, "evalue": 9.16e-175, "gene_start": 1, "gene_end": 286, "reference_start": 1, "reference_end": 286, "reference_sequence": "MKQKIKSRLKKDNWLRYLSQTVAVCFLLLFISFFIFLLIEAAKTGPDFTKSLLGLEFNLGAKKASIWFPLLVSFVVSIGSLIIASYIGVRTSIFLVYRCKPRIRKKLLLVIDILSGIPSVIFGLFATQILSSIFRDVLHLPPLSLLNVIVMLSFMIIPIVISLTTNALLHVESSLMTVAISLGENKTSVIYKVIKKEIKAQLVVILVLAFGRAISETMAVNFILQSVNYQEVIANDRFFTSDLKTLGSVISTFIFSENGDEQVSGVLYTFGIIIFVLISFLNFFAIWSTRPKTLERYPFLKKISNFIYQVVWFIPNNIGALFTDLTARRQQVKKITAANVEQRATFFKERMQTNHLNKVYTSWKILQEIFCAVLAFGFVLGILLFVFINGSQAIQRSGSTVFSFGVDTTGRALVNTLVIILVAIGITFPIALLIAIWLNEYTKSRIAKNTFSFVIDSLSSMPSIIYGLFGLSFFLRTLQLSAGGANGTSLMAGILTISVVVLPFLIRTCQEALNNVSWDLRVSAYALGVSKREVIWKIVLPGALKGLIIALILTINRIIAETAPFFITAGLASSNLFDLSLPGQTLTTRIYGQLFSTNSTAVDVMLETALVSIVFLMFLIFLSSYLIPYLFSFNKQKWLQIKSKLQLWKKA" }, "paralog": false, "identity": 0.9922480620155039, "score": 728.0, "evalue": 1.78e-264, "description": "Internal stop codon at 725268, 725496. Nonsense mutation around 725268, 725496.", "psc": { "uniref90_id": "UniRef90_P75185", "gene": "pstA", "product": "phosphate ABC transporter permease PstA", "uniref50_id": "UniRef50_P75185", "cog_id": "COG0581", "cog_category": "P", "go_ids": [ "GO:0005315", "GO:0005886", "GO:0006817", "GO:0015415", "GO:0035435", "GO:0055052" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75185", "UniRef:UniRef50_P75185", "COG:COG0581", "COG:P", "GO:0005315", "GO:0005886", "GO:0006817", "GO:0015415", "GO:0035435", "GO:0055052" ] }, "pscc": { "uniref50_id": "UniRef50_P75185", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75185" ], "product": "Phosphate transport system permease protein PstA homolog" } }, "id": "CNOCLNGIAL_1281", "locus": "GCA000143945_01281" }, { "type": "cds", "sequence": "contig_1", "start": 726354, "stop": 726530, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB5478A", "UniRef:UniRef90_UPI001FB5478A" ], "nt": "TTGTTATTTAGCAACAACAGTGAAATTAAGAAAATATACGATGACTTTGGTGTGTTAGAGTTAACGGCTGACGAAAAGAAAAAGATGTTTAAAACAGGTAATGCAGAAATGAGCAACATTGCCAACTTCTGGGTTGATGATTACAGTCTGAACAACCAAACCTTCGGTGCACTCTAG", "aa": "MLFSNNSEIKKIYDDFGVLELTADEKKKMFKTGNAEMSNIANFWVDDYSLNNQTFGAL", "aa_hexdigest": "db872504cdb65525c719520b4b55ed97", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F234BB1", "query_cov": 1.0, "subject_cov": 0.8529411764705882, "identity": 0.569, "score": 71.2, "evalue": 3.43e-15, "valid": false, "uniref50_id": "UniRef50_UPI001FB5478A" }, "pscc": { "uniref50_id": "UniRef50_UPI001FB5478A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB5478A" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 726354, "stop": 726599, "observations": { "mutation": [ 726533 ], "stop": [ 726533 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FB5478A", "query_cov": 1.0, "subject_cov": 0.7160493827160493, "identity": 0.983, "score": 118.0, "evalue": 8.42e-34, "gene_start": 1, "gene_end": 58, "reference_start": 24, "reference_end": 81, "reference_sequence": "MNSVVSLLPKQKTLPSIQRFFNWLLFSNNSEIKKIYDDFGVLELTADEKKKMFKTGNAEMSNIANFWVDDYSLNNQTFGAL" }, "paralog": false, "identity": 0.9753086419753086, "score": 159.0, "evalue": 1.73e-52, "description": "Internal stop codon at 726533. Nonsense mutation around 726533.", "psc": { "uniref90_id": "UniRef90_UPI001FB5478A", "uniref50_id": "UniRef50_UPI001FB5478A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FB5478A", "UniRef:UniRef50_UPI001FB5478A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FB5478A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB5478A" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6658.412000000002, "isoelectric_point": 4.563057136535646 }, "id": "CNOCLNGIAL_1282", "locus": "GCA000143945_01282" }, { "type": "cds", "sequence": "contig_1", "start": 726531, "stop": 727511, "strand": "-", "frame": 1, "gene": "pstS", "product": "putative lipo MG412-like protein", "db_xrefs": [ "COG:COG0226", "COG:P", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47652", "UniRef:UniRef90_P75184" ], "nt": "ATGCCAGTTTTTCTAAAATTAACGCACACAATTAGAAAAGTGCTAAGAGTTGCAAGACTTTCTAGACTAGCATTACTATCACTAACCGCTGTTATTTTTAGCGGTTGTGCCAATATTAATTTAATTAGTGCTGTTGGTTCTTCTTCGGTACAACCGTTGTTAAGCAAACTCAGTTCGCACTATGTCTTGAACCACAATGACAAGGATAACCTTGTAGAAATTAGTGTCCAAGCGGGTGGCTCTAGTGCTGGGGTGAAAGCAATCACCAAGGGACTAGCTGACATTGGTAATGTCTCGAAAAACACCAAGAGCTATGCTGAGGAAAACAAGCAGTTGTGGATGGACAAAAAGCTCAAAACAATTACACTTGGCAAAGATGCCATTGCTGTTATTTATAAAGCACCATCAGAGTTTAAGGGCAAACTAGTTCTCACTAAAGACAACCTCAACGATCTTTACGATCTGTTCGCTGGTAGCAAAAGCGTTGACATTAATAAGTTTGTCGAAAACGGACAAACCACTAAAAACAGTAATCATAATTTGATAGGCTTCCCCCGTACTGGGGGCGCTTTTGCTTCTGGTACCGCTGAAGCCTTCTTGAAGTTTTCGGGTCTTACACAGACAAAAACTTTAGATAAAGATTCCAAAGAAATTTTGGAAGGTCAACGCAACTATGGCCCCAATGCGCGACCAACTAGTGAAACCAACATTGAGGCCTTTAATACCTTTGTCACAACTTTGCGACAACCCAATTTATACGGCATGGTGTACCTCAGTTTAGGGTTTGTGAATAACAACATGAACCTAATTAAGAGTGAAGGTTTTGAGGTTTTAAAAGTCAAATATGATAATAACGCAGTTACCCCCTCCAGTCAAGCAGTTTCTAGCAACACTTACAAATGGGTACGCCCGTTGAACTCAGTGGTTTCCCTGTTACCAAAACAAAAAACACTGCCAAGTATCCAACGCTTTTTTAACTGA", "aa": "MPVFLKLTHTIRKVLRVARLSRLALLSLTAVIFSGCANINLISAVGSSSVQPLLSKLSSHYVLNHNDKDNLVEISVQAGGSSAGVKAITKGLADIGNVSKNTKSYAEENKQLWMDKKLKTITLGKDAIAVIYKAPSEFKGKLVLTKDNLNDLYDLFAGSKSVDINKFVENGQTTKNSNHNLIGFPRTGGAFASGTAEAFLKFSGLTQTKTLDKDSKEILEGQRNYGPNARPTSETNIEAFNTFVTTLRQPNLYGMVYLSLGFVNNNMNLIKSEGFEVLKVKYDNNAVTPSSQAVSSNTYKWVRPLNSVVSLLPKQKTLPSIQRFFN", "aa_hexdigest": "2d738190940de6080edb27631c107cab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75184", "query_cov": 1.0, "subject_cov": 0.8467532467532467, "identity": 1.0, "score": 620.0, "evalue": 3.3e-223, "valid": true, "gene": "PstS", "product": "Uncharacterized lipoprotein MG412 homolog", "uniref50_id": "UniRef50_P47652", "cog_id": "COG0226", "cog_category": "P", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47652", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47652" ], "product": "Uncharacterized lipoprotein MG412" }, "genes": [ "pstS" ], "id": "CNOCLNGIAL_1283", "locus": "GCA000143945_01283" }, { "type": "cds", "sequence": "contig_1", "start": 727753, "stop": 728700, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6450", "UniRef:UniRef100_A0AAX0SPZ2", "UniRef:UniRef50_A0AAX0SPZ2", "UniRef:UniRef90_A0AAX0SPZ2" ], "nt": "ATGGAAAAACTCAATGATTTTGAAAAGCAACAAATTGTTTTTGTCATTAAAAACAGTTTTGCTGGTGTTGATAAAACCGTGTTAGATGTAGATGCTAACAGCTTAACCTTTGTGGAAGATCCCCGACAATACAAACTTGATTTAGGAAACATGAACGAAAACAAAGGTTTCTTCTATGTGTTAGCCGACATCCACAACAGCAACGAACTGTTTAAGTTAGGTTCCACGATCGATCCCGAAATTATAAAAGGGCAAATGTACTTTGTTAAACAAAATCAAATAAAGCCCAACACACGCACTTACTTGTTCAAAATTAATACGCGCAAGCTGTTGCTTGACAAACAAAAGTTGTGGTTTAAACCCCACTTGGAAACCCAAACAGTGAGCTTTATGTTTGATGAATTCGAAATTGGCAAATTAGATATAGAAACATCCAACATCCAATTAATAGATGACTATGAGTTTGAGTTTCAATCAGACTTTAGTTTGCAAGATGAACAAGTGTTATTGGAAAAACTCAACATTGTTTTGAGTCAAATGAACCTCCATGTGGAAACGAAGAATGCTAGGTTGAATCCGGTAAATAAAATGGCTTATTTAACCGTTTTAAAAAACAACCAAAAGTTCAACTTAGAGTTCCATGTTGATCGCTATACTAGTCAGCTCTCCATTCAAGCAATGTTGGGTAGTCAAAGACTTTTTGCAATTAACTATGACTTAAAGCTATCACAAAATAAGCAAATGCTATATCTCATTAATAAAGATGGTCTAGAAAAGTCAGTTTGGTTCAACATTAAAAATGACAGTCAAACTAAGCTAGCACAACAACTAGCTAGATTCTTAAAGGAAAACAACTTCCAGTTTAGACAAAAACCAGTCTTTGATTTCCACACCCAAAACAAAGCCTTTGCACTGGAAAAGTTGGACAATAAAGATGACACTGATTAA", "aa": "MEKLNDFEKQQIVFVIKNSFAGVDKTVLDVDANSLTFVEDPRQYKLDLGNMNENKGFFYVLADIHNSNELFKLGSTIDPEIIKGQMYFVKQNQIKPNTRTYLFKINTRKLLLDKQKLWFKPHLETQTVSFMFDEFEIGKLDIETSNIQLIDDYEFEFQSDFSLQDEQVLLEKLNIVLSQMNLHVETKNARLNPVNKMAYLTVLKNNQKFNLEFHVDRYTSQLSIQAMLGSQRLFAINYDLKLSQNKQMLYLINKDGLEKSVWFNIKNDSQTKLAQQLARFLKENNFQFRQKPVFDFHTQNKAFALEKLDNKDDTD", "aa_hexdigest": "9c3d4f3fdc80a0549b0ed8a661e43592", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6450", "uniref100_id": "UniRef100_A0AAX0SPZ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6450", "UniRef:UniRef100_A0AAX0SPZ2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SPZ2", "uniref90_id": "UniRef90_A0AAX0SPZ2" }, "psc": { "uniref90_id": "UniRef90_A0AAX0SPZ2", "uniref50_id": "UniRef50_A0AAX0SPZ2" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SPZ2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37164.97450000004, "isoelectric_point": 6.034732246398924 }, "id": "CNOCLNGIAL_1284", "locus": "GCA000143945_01284" }, { "type": "cds", "sequence": "contig_1", "start": 728767, "stop": 728982, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACACCACAACAACACGCTTCCAACGAATACACCATTAACTTTTTAAGTTTGGATTTTATTGTTGACGGTTATGAATCAGAAAACTTGCACTTCCAAGTAGTTGATCAACTTTTTGGTGCAGTGAAAAACCTACCAACGATCAACAAAATTCCAGAAGGTTACCAGTACAAATTTAAATATCGCAGTAACTTTAGCAACCAGCAATTTAAATAA", "aa": "MTPQQHASNEYTINFLSLDFIVDGYESENLHFQVVDQLFGAVKNLPTINKIPEGYQYKFKYRSNFSNQQFK", "aa_hexdigest": "5c399516d4654f89bf44a7bf34839b3b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8382.256399999998, "isoelectric_point": 6.018874168395995 }, "id": "CNOCLNGIAL_1285", "locus": "GCA000143945_01285" }, { "type": "cds", "sequence": "contig_1", "start": 728992, "stop": 729390, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATACTCTGTCCAAAACATTTAATGAAAAAGATCTATCTCAAAAAGATCAATTACAAATAATGTTCAAAAAGGAGTTTACTCAAGCGATTGCCCAAATTAGTAAGTTGAACGAAGAGATTGATTTAGCTACTGATAAAGCAACAGCGCTACAAAAGTTTGACTCTTTAAAGTCCAAGGACAGCAACGTTAATTTTGCTAGCTTGCAGTACCTCTATGACTTATTAGGGATGGAGCCTACCAATAAAGAACACCTTATCTTCATTAGGAACTTACCAGAGATGTTGCAAACTATTTTTGCACGAGCCAAACTTTTAGCTAAAGTCAAAATTGGTGATACCTTGCAAGAAATTACGTTGCTTAAAAACAATGTCAATGTGTTTGATATCAAAACTTGA", "aa": "MDTLSKTFNEKDLSQKDQLQIMFKKEFTQAIAQISKLNEEIDLATDKATALQKFDSLKSKDSNVNFASLQYLYDLLGMEPTNKEHLIFIRNLPEMLQTIFARAKLLAKVKIGDTLQEITLLKNNVNVFDIKT", "aa_hexdigest": "3fa208e72ec2bab3245796d2e4678c48", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15157.384399999984, "isoelectric_point": 6.5873208999633786 }, "id": "CNOCLNGIAL_1286", "locus": "GCA000143945_01286" }, { "type": "cds", "sequence": "contig_1", "start": 729424, "stop": 729666, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCCTTTACTAGAAACCGCCAAGTAACCCAATCAATCTTGAATATGGGTAAAAACGTTGTAGTAAAGCCATTAACTGATGATGAGGAACAAAGTTTGTTTAGCAATAAGTGGGACATTTTCAAATACACCCCAATTATCCCAGTAAACGCTCAGCGCTTTGGTGTTGACTTTTATGTGGAACAACATAATGCAGATCTAAAACCACCTATTAGTTTTAGTCAACTTTCTAGGTTGTGA", "aa": "MEAFTRNRQVTQSILNMGKNVVVKPLTDDEEQSLFSNKWDIFKYTPIIPVNAQRFGVDFYVEQHNADLKPPISFSQLSRL", "aa_hexdigest": "898462f945d8a76462aab728de47360b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9302.476099999996, "isoelectric_point": 6.54571475982666 }, "id": "CNOCLNGIAL_1287", "locus": "GCA000143945_01287" }, { "type": "cds", "sequence": "contig_1", "start": 730159, "stop": 730455, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HPZ9", "UniRef:UniRef90_A0AB33HPZ9" ], "nt": "TTGCTTAGAGAAAATAACTTTAATTGGTATAACAATATCTATTACATCAAAGATGTTAACTTAGGCATTGAAAGTAGCTATACTAGCAGTTTGGCATGGAACAAAACTTTTAGCTATCCCGGAGTTAGAAACTCCTTTAAATCAGATATACACGACCTTCAGTATTTCCACTTTCACAATGATAGTAAGTCTGGTTTTGGACCATCTCGCATTAATGACCCGTGGTATCCACAGCATGATAAATATGTGACATTAAGCGCTGGTCGAACTGCCGAAGAAGCTAAAAACGCAGGATGA", "aa": "MLRENNFNWYNNIYYIKDVNLGIESSYTSSLAWNKTFSYPGVRNSFKSDIHDLQYFHFHNDSKSGFGPSRINDPWYPQHDKYVTLSAGRTAEEAKNAG", "aa_hexdigest": "7a72347229b26f1751ab42b255f19204", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 730029, "stop": 730746, "observations": { "insertion": [ 730077 ], "mutation": [ 730161, 730497, 730515 ], "stop": [ 730064, 730161, 730497, 730515 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HPZ9", "query_cov": 1.0, "subject_cov": 0.4117647058823529, "identity": 0.99, "score": 207.0, "evalue": 2.76e-66, "gene_start": 1, "gene_end": 98, "reference_start": 98, "reference_end": 195, "reference_sequence": "MRSYHYLPLLGFVAFPTLLANASLDKNLVDSSLYISNSAHHNQGFTVGIKLKDSYFRKDFNFENPLIYKPNNKTTFGWNYSTLWTQLADGYYDRYNQLLRENNFNWYNNIYYIKDVNLGIESSYTSSLAWNKTFSYPGVRNSFKSDIHDLQYFHFHNDSKSGFGPSRINDPWYPQHDKYVTLSAGRTAEEAKNAGWSIPYNLETQRLFSPVWNRNKDFKWINVFSVLWTVPYQLSHLT" }, "paralog": false, "identity": 0.9831932773109243, "score": 483.0, "evalue": 4.31e-177, "description": "Internal stop codon at 730064, 730161, 730497, 730515. Frameshift due to insertion around 730077. Nonsense mutation around 730161, 730497, 730515.", "psc": { "uniref90_id": "UniRef90_A0AB33HPZ9", "uniref50_id": "UniRef50_A0AB33HPZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HPZ9", "UniRef:UniRef50_A0AB33HPZ9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HPZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HPZ9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11434.36359999999, "isoelectric_point": 6.899025917053222 }, "id": "CNOCLNGIAL_1288", "locus": "GCA000143945_01288" }, { "type": "cds", "sequence": "contig_1", "start": 730513, "stop": 730746, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCTCATATCATTATTTACCTTTATTAGGTTTTGTTGCGTTCCCAACTCTTTTAGCGAACGCGTCGTTGGATAAAAATCTTGTTGATAGTAGTTTGTATATTTCTAACTCAGCACACCACAATCAAGGGTTTACTGTTGGGATCAAATTAAAGGATAGTTATTTCCGTAAAGACTTTAATTTCGAAAATCCTTTAATTTATAAACCAAATAACAAAACTACTTTTGGTTGA", "aa": "MRSYHYLPLLGFVAFPTLLANASLDKNLVDSSLYISNSAHHNQGFTVGIKLKDSYFRKDFNFENPLIYKPNNKTTFG", "aa_hexdigest": "ec624bcf87c076c6f7c13776da001dba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8801.906699999992, "isoelectric_point": 9.347158241271973 }, "id": "CNOCLNGIAL_1289", "locus": "GCA000143945_01289" }, { "type": "cds", "sequence": "contig_1", "start": 730861, "stop": 731523, "strand": "-", "frame": 3, "gene": null, "product": "putative MG415-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75182", "UniRef:UniRef90_P75182" ], "nt": "TTGCCTAACGTTGCTTTCTTTAACGATTACCAAGTAGATTTGGTTTCTACCTTAACGTTAGCTGATAAGCAACTTTTAATAAGAAGACTGAACCTTGCATTAGTTGGCATGAACTTAATGGTTGATGAACAAGCTACTAACATAGAAACTTTTCCCAAACAAATAAGACTCACTACAAAGTCAATAGATCAATCGTTTGATTTTGATGTTGAATTTAATCCAAAAGCTAGTGAACTTAATGTAATTGTTAAGGTTGACGAACAACCATTATTTAAGTTGGATTATGAACTAGTGATTAAAAATTCAAACACTCTACAACTTGTTAACCGCAACAAGCATTTAGGCATGTATATCTGGTCTGTTGCTGATCAACGATACCAAGCTAAATTGCTCGATACCTTGCAGTTATTTCTTCAAAGTAAGCAGATGTACTTAAAAGAAAAGATTCCGCTTAAACTGCAGGATAATACCGCAACTTTTAAAATCGCCAAACAGGCTCAACCAAATAATGGTGAAAATAAAAACACCAATTTTATTGGTTACTTAGTAATTGCTGCTAGTAGTCTGTTTTTAGTGGTAGTTGCTTTTAGTTTTTACTTTTACAAGCGCAAAAAGAGTTTGAAATCGCACAAACAAAAAGCAGTTGTCAAAACGGATAAATAA", "aa": "MPNVAFFNDYQVDLVSTLTLADKQLLIRRLNLALVGMNLMVDEQATNIETFPKQIRLTTKSIDQSFDFDVEFNPKASELNVIVKVDEQPLFKLDYELVIKNSNTLQLVNRNKHLGMYIWSVADQRYQAKLLDTLQLFLQSKQMYLKEKIPLKLQDNTATFKIAKQAQPNNGENKNTNFIGYLVIAASSLFLVVVAFSFYFYKRKKSLKSHKQKAVVKTDK", "aa_hexdigest": "c475fda39bb4f122e2c544a38bee10f7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 730861, "stop": 731823, "observations": { "mutation": [ 731595, 731730 ], "stop": [ 731595, 731730 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75182", "query_cov": 1.0, "subject_cov": 0.6395348837209303, "identity": 0.991, "score": 414.0, "evalue": 6.13e-144, "gene_start": 1, "gene_end": 220, "reference_start": 125, "reference_end": 344, "reference_sequence": "MVFSFQDDTALTVALNSIKLVKNPEELIPGLIPTNPKTGIFYIAAEITNSNNFFKWGTDSNREIIKGQMYFLKKETKIPTIRTYLFQMRTHKIALNANDVWFQANNDKVKVIVDGIELDQFDPKLPNVAFFNDYQVDLVSTLTLADKQLLIRRLNLALVGMNLMVDEQATNIETFPKQIRLTTKSIDQSFDFDVEFNPKASELNVIVKVDEQPLFKLDYELVIKNSNTLQLVNRNKHLGMYIWSVADQRYQAKLLDTLQLFLQSKQMYLKEKIPLKLQDNTATFKIAKQAQPNNGENKNTNFIGYLVIAASSLFLVVVAFSFYFYKRKKNLKSHKQKAVVKTDK" }, "paralog": false, "identity": 0.990625, "score": 620.0, "evalue": 1.08e-227, "description": "Internal stop codon at 731595, 731730. Nonsense mutation around 731595, 731730.", "psc": { "uniref90_id": "UniRef90_P75182", "product": "Uncharacterized protein MG415 homolog", "uniref50_id": "UniRef50_P75182", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75182", "UniRef:UniRef50_P75182" ] }, "pscc": { "uniref50_id": "UniRef50_P75182", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75182" ], "product": "Uncharacterized protein MG415 homolog" } }, "id": "CNOCLNGIAL_1290", "locus": "GCA000143945_01290" }, { "type": "cds", "sequence": "contig_1", "start": 731593, "stop": 731691, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACTTTCTCAAAAAAGAAACAAAGATTCCAACAATTCGTACTTACTTGTTTCAAATGAGAACACACAAAATTGCTTTGAATGCCAATGACGTTTGA", "aa": "MYFLKKETKIPTIRTYLFQMRTHKIALNANDV", "aa_hexdigest": "f55ad7917052de9d645a5a467e9e5e81", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3885.6002000000008, "isoelectric_point": 9.99467868804932 }, "id": "CNOCLNGIAL_1291", "locus": "GCA000143945_01291" }, { "type": "cds", "sequence": "contig_1", "start": 731728, "stop": 731895, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTTCATTTCAGGATGATACGGCATTAACTGTTGCCTTAAATTCAATCAAATTAGTTAAAAATCCTGAAGAACTAATTCCTGGTTTAATTCCGACAAATCCTAAAACGGGCATCTTCTATATTGCAGCAGAAATTACTAACAGCAATAATTTCTTTAAATGA", "aa": "MVFSFQDDTALTVALNSIKLVKNPEELIPGLIPTNPKTGIFYIAAEITNSNNFFK", "aa_hexdigest": "7d2cfff18e9a15ac49bde0fbe0e257d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6086.957300000002, "isoelectric_point": 4.93575038909912 }, "id": "CNOCLNGIAL_1292", "locus": "GCA000143945_01292" }, { "type": "cds", "sequence": "contig_1", "start": 731873, "stop": 732121, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTCTTTATTTGAATCTGCTAAAAATAAACCAACCATTCAAAAAACAAACCAGGGCATTCAATACCGTTTAAGGTTTCGCTCAAATTACAACGAACAGTATTTCAATAAATATCGTTTTAGCATTCCCGTCGTTAATAACGGCAAGGAGTTTAGCGTTTTAGACATCCAAGATAAAGAATTGACTGAAGAGCAGAAAAACCAAATAGCTTTTGTAATTAAAAATGGTTTTTTCATTTCAGGATGA", "aa": "MFSLFESAKNKPTIQKTNQGIQYRLRFRSNYNEQYFNKYRFSIPVVNNGKEFSVLDIQDKELTEEQKNQIAFVIKNGFFISG", "aa_hexdigest": "37a9782533401d3c2661c13c76776856", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9736.900599999997, "isoelectric_point": 9.520900535583493 }, "id": "CNOCLNGIAL_1293", "locus": "GCA000143945_01293" }, { "type": "cds", "sequence": "contig_1", "start": 732140, "stop": 732301, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGGCAATTAAGAAAGATTACTTTAGTTAAAAACAACAATAACGTTTTTAATCTAGGCCAGTTTGAACAATTCTTAAATTTAGATCAAATTAGCATTAATTTATTTGAAGTTGAATTTCTCTCTTTTGATTTCATATCAGATCAATATCCTAGTTGA", "aa": "MGGQLRKITLVKNNNNVFNLGQFEQFLNLDQISINLFEVEFLSFDFISDQYPS", "aa_hexdigest": "f92003b0e74c2a2f68530ec69285d987", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6186.908599999997, "isoelectric_point": 4.295857048034668 }, "id": "CNOCLNGIAL_1294", "locus": "GCA000143945_01294" }, { "type": "cds", "sequence": "contig_1", "start": 732450, "stop": 732809, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17BD43", "UniRef:UniRef90_UPI001F17BD43" ], "nt": "ATGGGTTTTTCATTAACCCCACTGTTGCTCTCTAGCGCAACTATCAATTCACATGTAATTGACAGCTCGTTTTACTTAACAAAAAATTTAGTAGAGAGCGAAAATCAATCACCTATTCAGTTCAATCAGCTATCGCGAATTATTTTAGTTCAATTAGAGTTTGACCCTGATTCAATAGTTGATAATACTTCTATCACCTTTAATAACCGAACGCTTGGCCAAAAAGCTCTTTTGAAATTACGTTTTAAAAGGGAATTTACCTTAGCGATTCAAGAAATTACAGAGCTTAATCAGTTAGTTGATCAAGCAATTGATAAAAATACAGTTTTACAAAATTTCTTAACGCTTAAAAAATGTTGA", "aa": "MGFSLTPLLLSSATINSHVIDSSFYLTKNLVESENQSPIQFNQLSRIILVQLEFDPDSIVDNTSITFNNRTLGQKALLKLRFKREFTLAIQEITELNQLVDQAIDKNTVLQNFLTLKKC", "aa_hexdigest": "861a1a510f04e736d81ff0f355b2e1c8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 732149, "stop": 732854, "observations": { "insertion": [ 732464 ], "mutation": [ 732818 ], "stop": [ 732436, 732818 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F17BD43", "query_cov": 0.9831932773109243, "subject_cov": 0.4936708860759494, "identity": 1.0, "score": 223.0, "evalue": 7.57e-72, "gene_start": 1, "gene_end": 117, "reference_start": 16, "reference_end": 132, "reference_sequence": "MNVKIAMPFLKYWKLMGFSLTPLLLSSATINSHVIDSSFYLTKNLVESENQSPIQFNQLSRIILVQLEFDPDSIVDNTSITFNNRTLGQKALLKLRFKREFTLAIQEITELNQLVDQAIDKNTVLQNFLTLKNVERKQQWERLSYLYKLLNFDFRDPQELSLVRDLPRLLKTIFESASINFQVRMGGQLRKITLVKNNNNVFNLGQFEQFLNLDQISINLFEFEFLSFDFISDQYPS" }, "paralog": false, "identity": 0.9829059829059829, "score": 435.0, "evalue": 5.14e-158, "description": "Internal stop codon at 732436, 732818. Frameshift due to insertion around 732464. Nonsense mutation around 732818.", "psc": { "uniref90_id": "UniRef90_UPI001F17BD43", "uniref50_id": "UniRef50_UPI001F17BD43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F17BD43", "UniRef:UniRef50_UPI001F17BD43" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F17BD43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17BD43" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13546.408699999978, "isoelectric_point": 5.740533638000489 }, "id": "CNOCLNGIAL_1295", "locus": "GCA000143945_01295" }, { "type": "cds", "sequence": "contig_1", "start": 732922, "stop": 733119, "strand": "-", "frame": 3, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF615P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniParc:UPI0001DD6453", "UniRef:UniRef100_A0AB38W6H7", "UniRef:UniRef50_P75435", "UniRef:UniRef90_P75180" ], "nt": "ATGGCTGAAATTGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCTGAAATCGAAATGCGCAAAAAGCAATTGGATTATTACTACCATTTAATCTTTAGCAAGATTGCTCACTTTTCCAAACAATTAGCTTAG", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIEMRKKQLDYYYHLIFSKIAHFSKQLA", "aa_hexdigest": "977700c6647ca33d66291832bb7844ef", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6453", "uniref100_id": "UniRef100_A0AB38W6H7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6453", "UniRef:UniRef100_A0AB38W6H7" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6H7", "uniref90_id": "UniRef90_P75180" }, "psc": { "uniref90_id": "UniRef90_P75180", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF615P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75435", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75435", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75435" ], "product": "Type I restriction enzyme MpnII specificity subunit" }, "genes": [ "hsdS" ], "id": "CNOCLNGIAL_1296", "locus": "GCA000143945_01296" }, { "type": "cds", "sequence": "contig_1", "start": 733578, "stop": 733670, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGCAAGGAATTTTAGCAGAAATTGAGCTAGATTTCCCACCTTTGCAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTCCGAGCTAA", "aa": "MQGILAEIELDFPPLQIQEKIATILDTFPS", "aa_hexdigest": "d6c3b8b447a38b294f3c45cb5f0eefd9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 26, "aa_cov": 0.8666666666666667, "hmm_cov": 1.0, "evalue": 1.1979485129540943e-06, "score": 24.307828903198242, "start": 3, "stop": 28 } ], "seq_stats": { "molecular_weight": 3371.8918000000003, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_1297", "locus": "GCA000143945_01297" }, { "type": "cds", "sequence": "contig_1", "start": 733848, "stop": 734027, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGTTGATTAAACGTTTCAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAACAATCCAGGAGAATTCCCTGTTTATTCATCAGCAACAGTTAATAACGGTGAAATCGGTAAGATAAAGGATTGTGATTTTAATGGAGAGTACGTCACTTGA", "aa": "MLIKRFKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGKIKDCDFNGEYVT", "aa_hexdigest": "148aa3d4c075baf84a3b0da6e83ab4d5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 60, "aa_cov": 0.9491525423728814, "hmm_cov": 0.9833333333333333, "evalue": 4.6107011240780867e-11, "score": 38.6718864440918, "start": 4, "stop": 59 } ], "seq_stats": { "molecular_weight": 6740.782700000003, "isoelectric_point": 9.263607215881347 }, "id": "CNOCLNGIAL_1298", "locus": "GCA000143945_01298" }, { "type": "cds", "sequence": "contig_1", "start": 734100, "stop": 734498, "strand": "-", "frame": 1, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627", "KEGG:K02996", "RefSeq:WP_014325637.1", "SO:0001217", "UniParc:UPI0001DD6454", "UniRef:UniRef100_A0AAV5N9T9", "UniRef:UniRef50_Q98Q72", "UniRef:UniRef90_P75179" ], "nt": "ATGGAAAAACAATCTTATTATGGACTTGGACGCCGTAAGTCTTCAAGTGCTAAAGTTTATCTAACGCCTACCCAAGACAAGGGTAAGATTACTGTTAACCGTCGTGATCCTAGTGAATACTTTCCCAATAAAATAGTGATCCAAGACATGGAACAACCTTTAGATCTCACTGATCTTAAAAAGAACTTTGATATCAATGTGGTAGTTAAAGGTGGTGGTTTTACCGGACAAGCTGGTGCAATTCGTTTAGGAATTGTTCGCGCGCTTTTACAGTTCAATCCAGAATTGAAAAAGATTCTCAAGTCCAAGAAACTAACTACCCGTGACAAGCGGGTTAAAGAACGGAAGAAGTTCGGTTTATACGGTGCCCGCCGTGCTCCACAATTTACCAAGCGTTAG", "aa": "MEKQSYYGLGRRKSSSAKVYLTPTQDKGKITVNRRDPSEYFPNKIVIQDMEQPLDLTDLKKNFDINVVVKGGGFTGQAGAIRLGIVRALLQFNPELKKILKSKKLTTRDKRVKERKKFGLYGARRAPQFTKR", "aa_hexdigest": "8710d2521cbc32120e7a560a0cc6fdf7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6454", "ncbi_nrp_id": "WP_014325637.1", "uniref100_id": "UniRef100_A0AAV5N9T9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6454", "RefSeq:WP_014325637.1", "UniRef:UniRef100_A0AAV5N9T9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9T9", "uniref90_id": "UniRef90_P75179" }, "psc": { "uniref90_id": "UniRef90_P75179", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q98Q72", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q72" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "CNOCLNGIAL_1299", "locus": "GCA000143945_01299" }, { "type": "cds", "sequence": "contig_1", "start": 734582, "stop": 734944, "strand": "-", "frame": 2, "gene": "rplM", "product": "50S ribosomal protein L13", "db_xrefs": [ "COG:COG0102", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625", "KEGG:K02871", "SO:0001217", "UniRef:UniRef50_P47657", "UniRef:UniRef90_P75178" ], "nt": "ATGCAAAAAACATCAATGCTAACAAAAGAACAGGCCAACAAGCGACGACAGTGGTATATTGTCGACGCTGCTGGGCTCGTTCTCGGTAAGTTAGCGGTTAAAGCGGCCGATCTTATTCGCGGCAAGAATAAAGTTGATTTCACCCCTAACCAAGATTGTGGTGATTACTTGATCATTATTAACAGTGATCAAGTTGTGTTGACTGGCAATAAAAAAGAAAACGAGTTTTGGTACCACCACTCCCAATACATTGGTGGCATTAAGAAGGTAAGTGGTCGTGATATGTTGAAAAAACAATCCGACAAACTGGTCTATAATGCTGTTAAGGGCATGTTACCTGATAACCGTTTAAGCCGTCGTTGA", "aa": "MQKTSMLTKEQANKRRQWYIVDAAGLVLGKLAVKAADLIRGKNKVDFTPNQDCGDYLIIINSDQVVLTGNKKENEFWYHHSQYIGGIKKVSGRDMLKKQSDKLVYNAVKGMLPDNRLSRR", "aa_hexdigest": "2cd7e8a41898bbd90dc239a949ba520f", "start_type": "ATG", "rbs_motif": "AATAA", "psc": { "uniref90_id": "UniRef90_P75178", "query_cov": 1.0, "subject_cov": 0.821917808219178, "identity": 1.0, "score": 243.0, "evalue": 3.32e-81, "valid": true, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_P47657", "cog_id": "COG0102", "cog_category": "J", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47657" ], "product": "Large ribosomal subunit protein uL13" }, "genes": [ "rplM" ], "id": "CNOCLNGIAL_1300", "locus": "GCA000143945_01300" }, { "type": "cds", "sequence": "contig_1", "start": 734975, "stop": 735196, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAAATTTTTCAACAAAACCTGTTCTTGAGTTCCTTTACTTTGAGTGAATATGAAACTAAGGTACTAGCTAAAGTGGAACAACTAACGCATAAACCCCAAGTGTTGCAATTACAACAGTTAGAACAGTTAAGTAGTACCGTTGTTAAAAAAGCTCAAAAAACAGCGGCACAGGAGATCGCTGATACGTTCTTCAAAGGGCTTTATGAAGAAAAGTAA", "aa": "MLEIFQQNLFLSSFTLSEYETKVLAKVEQLTHKPQVLQLQQLEQLSSTVVKKAQKTAAQEIADTFFKGLYEEK", "aa_hexdigest": "260cfc62722e2f3367007752bcb7a6c8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8435.6081, "isoelectric_point": 5.7392263412475595 }, "id": "CNOCLNGIAL_1301", "locus": "GCA000143945_01301" }, { "type": "cds", "sequence": "contig_1", "start": 735233, "stop": 737020, "strand": "-", "frame": 2, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_P47658", "UniRef:UniRef90_P75177" ], "nt": "ATGCGCAAGGTCCTTTACCAGAAATACCGTCCCACCAAGTTTAGCGATACTGTCGGTCAGGACAGCATTAAACGCATTATTGTTAATGCCATTACCCAAGACCAATTACCCCACGGTTACATCTTTGCGGGGGAGCGGGGTACGGGAAAAACGACCTTTGCCAAGATTATTGCCAAAGCCATTAACTGTTTAAACTGGAACGGCGATGTTTGCAACCAGTGTGAGGCATGCCAAGCGATTAACAGCAACAGTGCGATTGATGTGTTTGAAATTGATGCTGCTTCCAAAAACGGGATTAACGACATTAGGGAGCTAGCGGAAAATGTCTTTAACCTACCCTTTAAGTTCAAAAAGAAGGTTTACATTTTGGATGAAGCCCACATGCTCACCCCCCAATCTTGGAGTGGGCTTTTAAAGACCTTAGAAGAAGCACCCGATTATGTCTTGTTTATTTTTGCCACCACCGAATTTAACAAGATTCCGATCACGATCTTGTCGCGTTGCCAAAGCTTCTTCTTTAAACAAATTACTAACGATCTCATCCAGCAACGGTTAGCGGAAGTAGCGGCTAAGGAAAGCATTAAGATTACTACTGATGCTTTGGTCAAACTAGCTGATTTAGCGCAAGGTTCGTTGCGTGATGGGTTGAGCTTGTTGGACCAAATTAGTAACTTTAGTGAAAGTAAAACCATTAGTTTAGCTGATGTTGAAAAGACCTTTAACCTGCTTGACAAGGAACAAAAGTTTGGTTTTATTGAAGCAGTTTTAAGTGGTGATCTCAAACAATCCTTTCACTTAATTGACAACTTTGAAAGTCAAGGGATTAACTTTGTTCACTTTCTCAGGGAACTGTTTGCCCTAACAGTTGATTTATATGGTTATGTCAAAACGGGACAAATTGCAGTTGTAAAACCCAGCGACCAAACAATGGCGGCAAAGCTTCGTTTTCACCCCAAACAGTATGCTCTTTTGGTTCAAGCAATTGAAGCTAACAATGGTTATGGTCCTTCACAACTATCCTTAAGTGATCAGATTAAAGCAATTGTGATTCACTATAAAAATGCCGTTAGCAAAGAGCCACACATACCCGCTTATACGCCAGTGGTACAAACTCAATCACCAGCAAAAGAACCCGTTCCTTCAAAAACTGTAGAAGAGGCCAAGCCCCAACTACCAGCAAAAGAACCTGTTCTTTATAAAGTAATAGAAGAACCTAAAGTTTCATCATTCGTAAAAGATCCTGTTGCTTCTAAACTAATAGAACAACCCCAAACTATAGTGCAACCAGAAGCACAGCAAGAAATTACAGAAGTAGAACAACCAACAGAAACTTCACCAGCTCCAGCAACTGATTTATTTGGGTTAGCGATTAAACCAGAGGTGGTACGCAAACGCGGTCGTAGACCTCTTTCTGTAGAAAACACTGATTTTTTTCAGCCCGCTGTTAAAAAATTAATACAGCCTGTAAGCAAGCCTGCTCCTATTAAGTTAATAGAACCATCAGATAATAACCCAGTTTCCATCCCAATAAAACTTAACCGGGCTGTGATTGCCGTTTCTGTTTATGGGCACAATGATCCTAAACTAGTAGCCCACTTCCAACAGTTATTGGACAGCTTTAAAAGGGAGTTCACCCAAGCAGAAAAAACTAAAGACAGCAGTTACTTAAAGCAGTTTTCCGATAAGTTTACCGCTAGTGATTTAAGTAAGGCTATTAAGGTTCTAGCAGCATCACCCTTTGGTTTAGTCTTAATTTTCGAAGATAAAGAGATAGCAACAAGGTTGTGA", "aa": "MRKVLYQKYRPTKFSDTVGQDSIKRIIVNAITQDQLPHGYIFAGERGTGKTTFAKIIAKAINCLNWNGDVCNQCEACQAINSNSAIDVFEIDAASKNGINDIRELAENVFNLPFKFKKKVYILDEAHMLTPQSWSGLLKTLEEAPDYVLFIFATTEFNKIPITILSRCQSFFFKQITNDLIQQRLAEVAAKESIKITTDALVKLADLAQGSLRDGLSLLDQISNFSESKTISLADVEKTFNLLDKEQKFGFIEAVLSGDLKQSFHLIDNFESQGINFVHFLRELFALTVDLYGYVKTGQIAVVKPSDQTMAAKLRFHPKQYALLVQAIEANNGYGPSQLSLSDQIKAIVIHYKNAVSKEPHIPAYTPVVQTQSPAKEPVPSKTVEEAKPQLPAKEPVLYKVIEEPKVSSFVKDPVASKLIEQPQTIVQPEAQQEITEVEQPTETSPAPATDLFGLAIKPEVVRKRGRRPLSVENTDFFQPAVKKLIQPVSKPAPIKLIEPSDNNPVSIPIKLNRAVIAVSVYGHNDPKLVAHFQQLLDSFKREFTQAEKTKDSSYLKQFSDKFTASDLSKAIKVLAASPFGLVLIFEDKEIATRL", "aa_hexdigest": "30a4553e9061dba9d12a36c97762aee3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75177", "query_cov": 1.0, "subject_cov": 0.8737151248164464, "identity": 0.99, "score": 1030.0, "evalue": 0.0, "valid": true, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47658", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47658", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47658" ], "product": "DNA polymerase III subunit gamma/tau" }, "genes": [ "dnaX" ], "id": "CNOCLNGIAL_1302", "locus": "GCA000143945_01302" }, { "type": "cds", "sequence": "contig_1", "start": 737029, "stop": 738885, "strand": "-", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "nt": "TTGTCTTTAATTAACCACTATCAAATTCCCCTAGACCAACCCTTAGGTCAAATGGATACTGATTTAGTACGGATGATCTTGGAGGGTAGTGATGAACCAATTGAGATTAAAACAGTATCAAACTCAGGTGCCAAAAATGTGCGCTTTGAACACTATGAGGGTGTGGCTCACTTAATTAAACGCCGTCACTTAGAGACTAGTAGTCAAGCCAGTCGTGAATGGTACTCGGCTTACATGTCTGAAATTACCTGTAAGAAGTGCCAGGGCAAGAAGCTTACTACTAATTCTTTAAGTGTCAAGTTAGGTGGGTTGGACATTATTAGCTTTACGGAACTCTCGATTGATAAAGCAATTGAATTCTTGTTGCAAATAGAACTGAATCAAGAACAGAAGAAGATTGGTGAGTTAGCTCTTAAAGAGATTATTAACCGTTTATCCTTCTTGAAGAATGTGGGCTTAGAATACCTTAACTTAGCGCGCCGGGCTTCTACCCTGTCAGGTGGTGAAGCACAACGGATTCGCTTAGCCACCCAAATTGGTTCGCAACTCACTGGCGTCCTTTATGTCTTGGATGAACCCTCAATTGGGCTACACCAAAAGGACAATGATCGCTTAATTAACACGATGATGGTAATGCGGGATTTGGGCAACACCTTACTCGTGGTAGAACACGACAGTGAAACCATGCTAGCAGCTGATTACCTCATTGATATTGGTCCTAAAGCGGGTAACCAAGGTGGGGAAGTAGTAGCTGCTGGGACACCGCTAGAGGTAATGGAAAATACTGACTCCTTAACGGGACAGTATCTCAGTGGCAAAAAGCAAATTGAGGTACCAAAAACCCGGCATGCTGGTAATGGTCGCACCTTAACCTTAAAAGGAGCCAAGGGCAACAACTTAAAGAACATTAATGTCACTATTCCCTTAAATAAATTAGTACTAGTGACCGGGGTATCGGGTTCAGGGAAATCAACCTTAATTAACCAAACCTTAGTACCGATCTTGGAACGCTTGGTGAACTATAAGAATGTTAAACCTGCACCCTATAAGGAAATCATAGGGGTCAACCACATTGATAAGGTGGTAGTGGTGTCGCAAGATCCGATCGGTCGCACTCCCCGCTCTAACCCCGCTACCTATGTCAGTGTATTTGATGACATTAGGGAGTTGTTTGCCAACACTAAGGAAGCCAAGGCTCGGGGTTACACTAACTCGCGCTTTTCCTTTAATGTAGCCGGTGGGCGCTGTGATAAGTGTTTTGGTGATGGGGTCATTCGGATTGAAATGCACTTTTTACCCGATGTTTATGTGACGTGTGAAATGTGTGATGGCAAGAAGTACAATCCCCAAACCCTGGAAGTTAAGTACTTAGGCAAATCGATCTTTGATGTGTTACAGATGTCATGTCAGGAAGCTTATGATTTTTTTAAAGCAATTCCGAATATTGCGAGAAAGTTAAAACTACTCTGTGATGTCGGTCTGGAATACCTCCAGTTAGGTCTTAATGTTACCTTCTTGTCAGGTGGGGAAGCACAACGAATTAAACTAGCCAAGTTTCTCCAGAAAAAGGCAACTGGTAAAACCTTGTACGTCCTGGATGAACCTTCAACGGGACTACACATAGAAGACATTAATAAGCTGTTAACGGTGATCCAACGGATTATTAAAAACGGTGATAGTGTCATTATTATTGAGCATAACCTAGATATTATTAAGATGGCGGACTACATCATTGATTTGGGTCCTGAAGGTGGGGAAAAAGGTGGACAAATTATTGCCCAAGGCACACCAGAACAACTGTTAAATCAGGTCGATAAGTCCTACACTGCCCAGTATCTCGCTAAAATCTTAAAGTAA", "aa": "MSLINHYQIPLDQPLGQMDTDLVRMILEGSDEPIEIKTVSNSGAKNVRFEHYEGVAHLIKRRHLETSSQASREWYSAYMSEITCKKCQGKKLTTNSLSVKLGGLDIISFTELSIDKAIEFLLQIELNQEQKKIGELALKEIINRLSFLKNVGLEYLNLARRASTLSGGEAQRIRLATQIGSQLTGVLYVLDEPSIGLHQKDNDRLINTMMVMRDLGNTLLVVEHDSETMLAADYLIDIGPKAGNQGGEVVAAGTPLEVMENTDSLTGQYLSGKKQIEVPKTRHAGNGRTLTLKGAKGNNLKNINVTIPLNKLVLVTGVSGSGKSTLINQTLVPILERLVNYKNVKPAPYKEIIGVNHIDKVVVVSQDPIGRTPRSNPATYVSVFDDIRELFANTKEAKARGYTNSRFSFNVAGGRCDKCFGDGVIRIEMHFLPDVYVTCEMCDGKKYNPQTLEVKYLGKSIFDVLQMSCQEAYDFFKAIPNIARKLKLLCDVGLEYLQLGLNVTFLSGGEAQRIKLAKFLQKKATGKTLYVLDEPSTGLHIEDINKLLTVIQRIIKNGDSVIIIEHNLDIIKMADYIIDLGPEGGEKGGQIIAQGTPEQLLNQVDKSYTAQYLAKILK", "aa_hexdigest": "08c69f143fb9187deb756f3e8ab47b77", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGE6", "query_cov": 0.8187702265372169, "subject_cov": 0.9980276134122288, "identity": 0.706, "score": 716.0, "evalue": 6.19e-254, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0PGE6", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PGE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "product": "UvrABC system protein A" }, "genes": [], "id": "CNOCLNGIAL_1303", "locus": "GCA000143945_01303" }, { "type": "cds", "sequence": "contig_1", "start": 738895, "stop": 739245, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGACCGGATTGTCATTAACCAAGACAAGCAAACCTTGGGTCGGATTGTTGATGCGATTGAAGGTGTGATTAAACTAACTGAGGGTCGGATTGAAGTGTTGTTGGAAGATGGTAAGATACTTAGTTTTAATAAGAACCACGGTTGTGATCAGTGTGGTTTTTCAATTAGTGAACTAGAGCCACGCTTATTCTCCTTTAACTCCCCTTTAGGTTCGTGTGAGTACTGTAAGGGTTTAGGTTTTAGTTATGAACCGGATGTGGAAAAGATTATTCCCAATCCGTTGTTGTCCATCAATGAAGGTGGTATTGACATCTTTAAGAACATAGTGCACGGTACATCACTAGATTGA", "aa": "MDRIVINQDKQTLGRIVDAIEGVIKLTEGRIEVLLEDGKILSFNKNHGCDQCGFSISELEPRLFSFNSPLGSCEYCKGLGFSYEPDVEKIIPNPLLSINEGGIDIFKNIVHGTSLD", "aa_hexdigest": "c864bc54e6c03a8afa32be3527d46d3a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12827.505099999984, "isoelectric_point": 4.7428388595581055 }, "id": "CNOCLNGIAL_1304", "locus": "GCA000143945_01304" }, { "type": "cds", "sequence": "contig_1", "start": 739519, "stop": 739875, "strand": "-", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0G0SCP6", "UniRef:UniRef90_A0A0G0SCP6" ], "nt": "TTGAAACCAGAGCTAAAAACTAATGATTTTATTCGCGTTAAGGGCGCGCGTGAAAACAACCTGAAGAATGTAAACATCGATATTCCCAAAAACCAGTTTGTCGTTATTACGGGGTTGTCGGGTTCGGGTAAATCATCACTTGCCTTTAACACCATTTACGCGGAAGGCCGCCGTCGGTATTTGGAATCACTCTCGTCATATGCCAGACAGTTTCTCGGTAACAGTGATAAACCGGATGTAGATTCGATTGAAGGGTTATCCCCAGCCATTTCAATTGACCAAAAAACCACTTCACACAATCCCCGCTCCACTGTGGGAACAGTAACGGAAATTTACGATTACCTCCGTTTACTGTGA", "aa": "MKPELKTNDFIRVKGARENNLKNVNIDIPKNQFVVITGLSGSGKSSLAFNTIYAEGRRRYLESLSSYARQFLGNSDKPDVDSIEGLSPAISIDQKTTSHNPRSTVGTVTEIYDYLRLL", "aa_hexdigest": "3e2ffd1f63e310734d00ad5da4e8ac46", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_X0QG93", "query_cov": 0.940677966101695, "subject_cov": 0.8809523809523809, "identity": 0.82, "score": 178.0, "evalue": 9.2e-56, "valid": false, "product": "UvrABC system protein A", "uniref50_id": "UniRef50_X0QG93" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 739216, "stop": 739848, "observations": { "mutation": [ 739521 ], "stop": [ 739521 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A0G0SCP6", "query_cov": 0.923728813559322, "subject_cov": 0.40977443609022557, "identity": 0.835, "score": 186.0, "evalue": 6.48e-57, "gene_start": 10, "gene_end": 118, "reference_start": 4, "reference_end": 112, "reference_sequence": "MEQFIKIRGARQHNLKNVDLDIPKNKLVVLTGVSGSGKSSLAFDTLYAEGQRRYVESLSSYARQFLGIMEKPDVDSIEGLSPAISIDQKTTSHNPRSTVGTVTEIYDYLRLLFARIGHPHCPVCGREISHQTLDQIVDQTMNLIETTAKADKKAWFLVLSPMVVARKGEFSQLFENLKSKGYRQVRIDGFIHDLSEDLILIKTNKHTIEAVTDRVALSADSLKGQPSKAELKNRLADSIQQALNLSEGMLVLAHVQDKEFTQYQSS" }, "paralog": false, "identity": 0.5806451612903226, "score": 247.0, "evalue": 2.34e-83, "description": "Internal stop codon at 739521. Nonsense mutation around 739521.", "psc": { "uniref90_id": "UniRef90_A0A0G0SCP6", "product": "UvrABC system protein A", "uniref50_id": "UniRef50_A0A0G0SCP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A0G0SCP6", "UniRef:UniRef50_A0A0G0SCP6" ] } }, "id": "CNOCLNGIAL_1305", "locus": "GCA000143945_01305" }, { "type": "cds", "sequence": "contig_1", "start": 739853, "stop": 740116, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTAGCGATTTAAATAAAATTAACACCAAGCAACGCTTTAAACTTTACCTGTTATTTAAGCTATTGTTACACCCCCAGTTGGTGTTGGTCGACTCCTTTGTCAACTTTAACAAACACACTTATAACTTTACCCGTGGTTTGTTAATTGCCCACCAAAACCAACAAGGCATTGCATATTTATTTAACGATCCGCACAATAACCTCGTAAAGGATTTTTTCACCCAAACAATTAACTTTGAAACCAGAGCTAAAAACTAA", "aa": "MIFSDLNKINTKQRFKLYLLFKLLLHPQLVLVDSFVNFNKHTYNFTRGLLIAHQNQQGIAYLFNDPHNNLVKDFFTQTINFETRAKN", "aa_hexdigest": "adb64aeb952abc6ab0434b9db13fe4f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10373.862199999989, "isoelectric_point": 9.820742988586424 }, "id": "CNOCLNGIAL_1306", "locus": "GCA000143945_01306" }, { "type": "cds", "sequence": "contig_1", "start": 740117, "stop": 740437, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTACAAAAAAGAAATTAAGCAAAAGCAACAGCAAATTAAAGCACTGCTAAAAAATTATGGCACTTTAAATAAGCTGCTCAATACTCAAATTAGTAAAGTCAATAACGTGGTACGCAAAACCTTCTTTGTGGATTCAGAAGAGTGTGATTTAAACCGTTTACAGGCATCGAATAAGCTGCACTTTAATCTCTTAAACGCGATGGTGAATGTCATTAGTTTTTGTCTCAAAAAGTGCCGGCAAAATCCCAAAAAACTAAACCGTACTGCTAACCTCAAAATGCTGTTGGATAACACCTTTAAAAACGGTATTCCGAGCTGA", "aa": "MYKKEIKQKQQQIKALLKNYGTLNKLLNTQISKVNNVVRKTFFVDSEECDLNRLQASNKLHFNLLNAMVNVISFCLKKCRQNPKKLNRTANLKMLLDNTFKNGIPS", "aa_hexdigest": "405d74d65708e6fa8d928a0ccd470747", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12328.422499999982, "isoelectric_point": 10.112978172302242 }, "id": "CNOCLNGIAL_1307", "locus": "GCA000143945_01307" }, { "type": "cds", "sequence": "contig_1", "start": 740486, "stop": 740635, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAAAACAGTTAATAAATACTTCGAAGCCAACTTGCACCACCCTCAAGCTTACTTGTTGTTACCTAATCGTAATGCCACTACTTTAACAGAAGCAAACACCACTACCATAAACCAACTTAACTTAAAAACCCAGCTCAAACAGTGA", "aa": "MDKTVNKYFEANLHHPQAYLLLPNRNATTLTEANTTTINQLNLKTQLKQ", "aa_hexdigest": "1b3e1ae7f1d1d2e8b3a0e3be866d8aef", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5655.358000000001, "isoelectric_point": 9.303061866760252 }, "id": "CNOCLNGIAL_1308", "locus": "GCA000143945_01308" }, { "type": "cds", "sequence": "contig_1", "start": 740660, "stop": 740791, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACAAACAAACACAACACTTCTTTACCAAATACCAGTTTATTACCCATGGTGTGGTTGACTTTGTCTTTAACCAAGGTCGGAATCAAACTCAGTTTGCCAGCTTAAAGGCCAACTTAAACCGCGATTGA", "aa": "MHKQTQHFFTKYQFITHGVVDFVFNQGRNQTQFASLKANLNRD", "aa_hexdigest": "26159408fa34c49b1761e9617fcb62e9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5143.711000000001, "isoelectric_point": 9.992099952697757 }, "id": "CNOCLNGIAL_1309", "locus": "GCA000143945_01309" }, { "type": "cds", "sequence": "contig_1", "start": 740846, "stop": 741208, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAGTTAACTAAGCCAATTGTTTTGACTCATGATGCGGTGATTGGTTTTTTAATTAAATCTCGTTTAGCTTTTTTGCAAGAATACGCTAAGGGCTTAAATCGCTGTGAGCAACACAACAAGCTGGTCAATGAGTTAAAGCAAAACGTTCTGTTAAACCAAAACCAGTATAAGGGTGAGGTTAGCCAAAGCTATTCGGTTGTAAACCAAAAGGCGGTTTTTAAGGGTTTTATCCAAACAGTTAAAGCAGCGCTTAACTACACTAAGTTAAAACACACCTTAGATCCGTTTAACCTAATGAATATTGTTCAAGAACGCTGTTTTGAACAACTCTTAACTACTTACGAAAAATTAGATTGA", "aa": "MEKLTKPIVLTHDAVIGFLIKSRLAFLQEYAKGLNRCEQHNKLVNELKQNVLLNQNQYKGEVSQSYSVVNQKAVFKGFIQTVKAALNYTKLKHTLDPFNLMNIVQERCFEQLLTTYEKLD", "aa_hexdigest": "9084ed01dc385e38f2f74d0a8d14c4ac", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13918.025699999986, "isoelectric_point": 9.250649070739744 }, "id": "CNOCLNGIAL_1310", "locus": "GCA000143945_01310" }, { "type": "cds", "sequence": "contig_1", "start": 741233, "stop": 741367, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCTTACTTAATCCCGATTTGTTAGTGAGCACAATTGCTGAAAGTGAGCGCCTAGTAGAACAGTTAATGAGCAACATCCAGCGCTTGTACCAGCAAATTTTGCAGCTAAAACCAGGTCAATCACTTAACTGA", "aa": "MRLLNPDLLVSTIAESERLVEQLMSNIQRLYQQILQLKPGQSLN", "aa_hexdigest": "561e64c6286c3fb9f7101f765077ed75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5094.9057999999995, "isoelectric_point": 6.014099693298339 }, "id": "CNOCLNGIAL_1311", "locus": "GCA000143945_01311" }, { "type": "cds", "sequence": "contig_1", "start": 741389, "stop": 741958, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCTTTAATGCACTCAAGAAGTTAAGCCAGTTTGTGGTGTTTGAACTAGATAACACCAATAAGTTAACGAGTGAAACACTTAGTCAGTTCTTCACCACTAAGTTTGTCAATAAGGTGTACTTTAACCAGATCCAGTTTGAGTACAACAACTTTGTTGGTTTATTGGAAGACCATGAACGTGACCTAGTGGAATTTAGCACCCACTTCTTTAACAGCTTCTTTGAACAACCTGATCACTTAACTAAAAGTTTTTTTGAACAGTACTATGCGCTTACAAGACAACGAGAACGTTTACTGCACAATTTAAAAGTTCAAAGCTATGAAACCAAACACGGGGTTAATACCTACTTTGAAAATTTGCAAATTACCCGTGCACAACAGCAAGCCAATCAGCTTAAGCGTGCCTTCAAAAAACAGCAGCGCAATTCGGTGCAGCTGATTAACCGCTTTATGTCGTCCTTGTGGTGGAGTCAAACCAAGATTAAGTTCAAGAGTATCTTTAACTTTTACAAAACCCGGATTTTGGAAAAACGGATTGTAGCCAAGCGAATCCAAATCAAAATTTGA", "aa": "MGFNALKKLSQFVVFELDNTNKLTSETLSQFFTTKFVNKVYFNQIQFEYNNFVGLLEDHERDLVEFSTHFFNSFFEQPDHLTKSFFEQYYALTRQRERLLHNLKVQSYETKHGVNTYFENLQITRAQQQANQLKRAFKKQQRNSVQLINRFMSSLWWSQTKIKFKSIFNFYKTRILEKRIVAKRIQIKI", "aa_hexdigest": "eadd1df54e193b03ba5c58100650fccf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22985.023500000014, "isoelectric_point": 9.958447456359863 }, "id": "CNOCLNGIAL_1312", "locus": "GCA000143945_01312" }, { "type": "cds", "sequence": "contig_1", "start": 742418, "stop": 744103, "strand": "-", "frame": 2, "gene": "rnjA", "product": "putative MG423-like protein", "db_xrefs": [ "COG:COG0595", "COG:J", "GO:0003723", "GO:0005886", "GO:0046872", "RefSeq:WP_010874978.1", "SO:0001217", "UniParc:UPI00001397B4", "UniRef:UniRef100_P75174", "UniRef:UniRef50_P47662", "UniRef:UniRef90_P75174" ], "nt": "ATGGCTAAAATTAACTTCTTTGCCTTCGGTGGTCAGGACGAGAGGGGCAAGAATTGTTTTGTTCTCGAAATCAATAACGATGTTTTTATTTTTAACGTTGGTAGTTTGACACCAACAACCGCTGTTTTAGGGGTCAAAAAGATCATCCCTGACTTTAGTTGGATCCAGGAAAACCAGGCCCGCATTAAGGGCATCTTTATCGGTAATCCAGTAACGGAAAACATTGGTTCGCTGGAGTTCTTATTCCACACCGTTGGTTTCTTTCCGATTTACACCAGTACGATTGGCGCTGTGGTTATTAAAACTAAGATCCACGAAAACAAGCTCAATATTCCCCATGACGAGTTGGAAATCCACGAGCTTAAACCGTTAGAAACGGTCAAGATCGGGCACCATAACATTACGCCCTTTAAGGTATCGAGTTCGATTCCTTCCTCGTTTGGCTTTGCGCTCCACACTGATGATGGGTACATTGTCTATGTCGATGACTTCATTGTGCTCAATGATAAGAACATCGCCTTTGAAAACCAACTCAATCAGATTATTCCCCAAGTAGCGAACAAGACCCTTTTGTTAATTACTGGAGTTGGTCTAGTGGGACGTAACACTGGTTTTACAACCCCTAAGCACAAATCCTTGGAACAACTCAACCGCATTATTGCCTCCGCTAAGGGTCGGGTATTTGCTGCTTGTTATGATTCCAACGCTTACAGTGTGATGACCTTGGCCCAAATTGCACGGATGCAAAACCGCCCCTTTGTCATCTACTCCCATTCATTTGTCCACCTCTTCAACGCGATTGTGCGCCAAAAGCTGTTTAACAACACCCACTTAAACACTATTTCGATTGAAGAGATTAACAACTCCACAAACGCCATTGTTGTTTTAACCGCGCCACCAGATAAGTTGTATGCCAAGCTGTTTAAGATTGGTACTAATGAGGATGAACGGGTACGGTATCGCAAGACCGATAGCTTTATCTTCATGATTCCCCGCATAGCTGGGTATGAAGAGTTGGAAGCACAAATCTTGGACGATGTCGCGCGTAATGAGGTGAGTTACTACAACTTGGGTCGGGAAATCTTGTCGATCAATGCATCGGATGAAGACATGAAGTTTTTAGTAACTTCCTTAAAACCTAAGTACATTATTCCCACATCCGGTCTGTACCGTGACTTCATTAACTTTACCATGGTAATGAAGCAAGCTGGTGTGGAACAAAGTCAGGTCTTAATTCCCTTTAATGGTGAAGTCCTAGCCATTAACCACAAGCAAATTGACAACAAAAAACGGGAATTAAAGCTCAACCCTAAGTGTGTTGACTCCGCTGGTTTGCAGGAAATAGGGGCCAGCATCATGTTTGAGCGTGATCAGATGTCTGAAGCGGGTGTGGTTACCATCATCATCTACTATGACTCCAAGAAGAGTGAGTTCCTAAACGAAATTACCTATTCCTTTTTAGGAGTTAGCCTAGATAGTAACAATCAGGTTAAACTCAAGACCAAAATGGAAGAGTTAATTCGCAAACAAATTAACGACATTAAGGACTTTACGACAATTAAGCGACGTTTAGGCAAAGACACATCGAAGGAACTCAAGGTTTCTATTAAACGAGCTGTCATGAATTTATTTACCAAAATGACTGCTAAAGCACCACTAATCCTGTCAACTATTATTTCTATTTAG", "aa": "MAKINFFAFGGQDERGKNCFVLEINNDVFIFNVGSLTPTTAVLGVKKIIPDFSWIQENQARIKGIFIGNPVTENIGSLEFLFHTVGFFPIYTSTIGAVVIKTKIHENKLNIPHDELEIHELKPLETVKIGHHNITPFKVSSSIPSSFGFALHTDDGYIVYVDDFIVLNDKNIAFENQLNQIIPQVANKTLLLITGVGLVGRNTGFTTPKHKSLEQLNRIIASAKGRVFAACYDSNAYSVMTLAQIARMQNRPFVIYSHSFVHLFNAIVRQKLFNNTHLNTISIEEINNSTNAIVVLTAPPDKLYAKLFKIGTNEDERVRYRKTDSFIFMIPRIAGYEELEAQILDDVARNEVSYYNLGREILSINASDEDMKFLVTSLKPKYIIPTSGLYRDFINFTMVMKQAGVEQSQVLIPFNGEVLAINHKQIDNKKRELKLNPKCVDSAGLQEIGASIMFERDQMSEAGVVTIIIYYDSKKSEFLNEITYSFLGVSLDSNNQVKLKTKMEELIRKQINDIKDFTTIKRRLGKDTSKELKVSIKRAVMNLFTKMTAKAPLILSTIISI", "aa_hexdigest": "d8eed88c141103209b004c9f0e6aed2d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001397B4", "ncbi_nrp_id": "WP_010874978.1", "uniref100_id": "UniRef100_P75174", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001397B4", "RefSeq:WP_010874978.1", "UniRef:UniRef100_P75174" ] }, "ips": { "uniref100_id": "UniRef100_P75174", "uniref90_id": "UniRef90_P75174" }, "psc": { "uniref90_id": "UniRef90_P75174", "gene": "RnjA", "product": "Uncharacterized protein MG423 homolog", "uniref50_id": "UniRef50_P47662", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0046872", "GO:0003723" ] }, "pscc": { "uniref50_id": "UniRef50_P47662", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47662" ], "product": "Uncharacterized protein MG423" }, "genes": [ "rnjA" ], "id": "CNOCLNGIAL_1313", "locus": "GCA000143945_01313" }, { "type": "cds", "sequence": "contig_1", "start": 744108, "stop": 744368, "strand": "-", "frame": 1, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_010874979.1", "SO:0001217", "UniParc:UPI0000134D13", "UniRef:UniRef100_P75173", "UniRef:UniRef50_P47663", "UniRef:UniRef90_P75173" ], "nt": "ATGCAAATTGATAAAAACGGGATCATTAAATCCGCACAACTCCACGACAAGGACGTGGGTAGTATTCAGGTACAAGTGTCCCTTTTAACGAGCCAAATTAAACAGCTCACCGACCACTTACTAGCTAACAAGAAGGACTTTATTTCCAAACGTGGTTTGTACGCGAAAGTATCAAAGCGCAAGCGCTTGTTGAAGTACTTAAAGCACAACGATTTAGAGGCTTACAGAAATTTAGTTAAAACCTTAAATCTCCGGGGTTAG", "aa": "MQIDKNGIIKSAQLHDKDVGSIQVQVSLLTSQIKQLTDHLLANKKDFISKRGLYAKVSKRKRLLKYLKHNDLEAYRNLVKTLNLRG", "aa_hexdigest": "e53f2d5f814b7b149311b178fd0d2b9b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134D13", "ncbi_nrp_id": "WP_010874979.1", "uniref100_id": "UniRef100_P75173", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134D13", "RefSeq:WP_010874979.1", "UniRef:UniRef100_P75173" ] }, "ips": { "uniref100_id": "UniRef100_P75173", "uniref90_id": "UniRef90_P75173" }, "psc": { "uniref90_id": "UniRef90_P75173", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P47663", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47663" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "CNOCLNGIAL_1314", "locus": "GCA000143945_01314" }, { "type": "cds", "sequence": "contig_1", "start": 744526, "stop": 744867, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "ATGGACTCAACTTTTAATGAACTAGGCGTCTCTCCTGCCTTAATTGCCACACTCAAAGATAACAACATTAACCAACCAACTACGATTCAACAACTGGCTATTCCCCAGTTTCTCCAACATCAAAACCTGATTGTCCACTCCCCCACCGGCACTGGTAAAACCGCTGTTTTTGCCATTCCGGTCATTGAAACACTCCTTAAAAAACCAAGTAAAGGTACGACCCAAACATTGGTAGTTGCACCTACCCGTGAACTGGCCGAACAGATTAAAACCACCTTTATTAACTTTGCTAAACACACCCATTTAAAGGTAGTGAGCTTAATTGGTGGCATCCCCATTTGA", "aa": "MDSTFNELGVSPALIATLKDNNINQPTTIQQLAIPQFLQHQNLIVHSPTGTGKTAVFAIPVIETLLKKPSKGTTQTLVVAPTRELAEQIKTTFINFAKHTHLKVVSLIGGIPI", "aa_hexdigest": "6f28b63d51c4fb90203bf0d1d38e6b1a", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 84, "aa_cov": 0.7433628318584071, "hmm_cov": 0.9761904761904762, "evalue": 4.805507714425576e-25, "score": 83.68274688720703, "start": 27, "stop": 110 } ], "seq_stats": { "molecular_weight": 12220.109599999982, "isoelectric_point": 9.523156929016114 }, "id": "CNOCLNGIAL_1315", "locus": "GCA000143945_01315" }, { "type": "cds", "sequence": "contig_1", "start": 744916, "stop": 745863, "strand": "+", "frame": 1, "gene": "srmB", "product": "putative ATP-dependent RNA helicase MG425-like protein", "db_xrefs": [ "COG:COG0513", "COG:L", "EC:3.6.4.13", "GO:0003689", "GO:0003723", "GO:0003724", "GO:0005524", "GO:0005829", "GO:0016887", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "SO:0001217", "UniRef:UniRef50_P47664", "UniRef:UniRef90_P75172" ], "nt": "ATGGGGCGGGTGATGGACCTGTTAGAACGGGGTGTAATTAAGTTTGAACACCTAGAACACCTCATTATTGACGAGGTTGACCTAATGCTCGACCGTGGTTTTAAGAGAAAACTGTTTGATCTGTTAGGTCGCATTGAAAAGTTTGAACAAATTGCGGTTTATTCTGCTAGTTACAATGAAGAGACAATTGAAACAGCTAAACAAATTACCAAGAATGGTATTTTCCTAGCAGCACCAGAATTAAAACAAAATGCCCCTGAACCAGATAACAAACTCATTGATCAATTTGTTTGTTATCTTTTTTCTAACCGGAAGAAACAAGCCCTCTATTCTTTGGTATCGCAAACTAGAGCGAAGTCGATTATTGTTTTTTGTGACACCAAGAAGTTAGTTGATGAGCTGTGCATCTTCTTGCGCAAGAATGATGTCAAAACTTATCCACTTCACGGGGACAAAGCGCAGTTCATTCGCGAACGCAACTTAAAGCTGTTTGCTAACACAACTGCTCCTATAGTATTAGTAACAACTGATCTAATCGGTCGTGGTATCCATGTGGAAGGGGTGGACATGGTAGTCAACTATTCCGCTTGTGTAAACTTTGAAACTTATTTGCACCGGATGGGTCGCACAGGACGGAACAACCACAAGGGCTCATGTATTACCTTCTGTACCTCACACGAAAAGCAAGCATTTTTAAAGTTATTAGAACAAGTTAACGACAAGCGCATTAGCCCTCTCCGCCCCATGCGCTTAAGGTTAATTCCCTTGAAGTGCAAAACCCAACCCAAGAAGGGGAAACTTTCCTTACAGTCAGTGCAAAAGATTTACGTCAATCCTCGTTCCAATGGCACCTTTAAACGCGTACCTTTAGCGGGGGATTTGTTTAAAAGCCGGATGCGTCAACCTGAGCGGGACATGCAAAAGAACAAATTGCATGACAGTGATTGA", "aa": "MGRVMDLLERGVIKFEHLEHLIIDEVDLMLDRGFKRKLFDLLGRIEKFEQIAVYSASYNEETIETAKQITKNGIFLAAPELKQNAPEPDNKLIDQFVCYLFSNRKKQALYSLVSQTRAKSIIVFCDTKKLVDELCIFLRKNDVKTYPLHGDKAQFIRERNLKLFANTTAPIVLVTTDLIGRGIHVEGVDMVVNYSACVNFETYLHRMGRTGRNNHKGSCITFCTSHEKQAFLKLLEQVNDKRISPLRPMRLRLIPLKCKTQPKKGKLSLQSVQKIYVNPRSNGTFKRVPLAGDLFKSRMRQPERDMQKNKLHDSD", "aa_hexdigest": "eae0cc757cc6827ce282a203a09dbc70", "start_type": "ATG", "rbs_motif": null, "genes": [ "srmB" ], "truncated": "both", "pseudogene": { "start": 744616, "stop": 745878, "observations": { "mutation": [ 744865, 745861 ], "stop": [ 744865, 745861 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75172", "query_cov": 1.0, "subject_cov": 0.7, "identity": 0.997, "score": 624.0, "evalue": 9.71e-224, "gene_start": 1, "gene_end": 315, "reference_start": 131, "reference_end": 445, "reference_sequence": "MDSTFNELGVSPALIATLKDNNINQPTTIQQLAIPQFLQHQNLIVHSPTGTGKTAVFGIPVIETLLKKPSKGTTQTLVVAPTRELAEQIKTTFINFAKHTHLKVVSLIGGIPIWQQLKQLENQPEIVVGTMGRVMDLLERGVIKFEHLEHLIIDEVDLMLDRGFKRKLFDLLSRIEKFEQIAVYSASYNEETIETAKQITKNGIFLAAPELKQNAPEPDNKLIDQFVCYLFSNRKKQALYSLVSQTRAKSIIVFCDTKKLVDELCIFLRKNDVKTYPLHGDKAQFIRERNLKLFANTTAPIVLVTTDLIGRGIHVEGVDMVVNYSACVNFETYLHRMGRTGRNNHKGSCITFCTSHEKQAFLKLLEQVNDKRISPLRPMRLRLIPLKCKTQPKKGKLSLQSVQKIYVNPRSNGTFKRVPLAGDLFKSRMRQPERDMQKNKLHDSDWQSNM" }, "paralog": false, "identity": 0.9904761904761905, "score": 822.0, "evalue": 2.64e-304, "description": "Internal stop codon at 744865, 745861. Nonsense mutation around 744865, 745861.", "psc": { "uniref90_id": "UniRef90_P75172", "gene": "SrmB", "product": "Probable ATP-dependent RNA helicase MG425 homolog", "ec_ids": [ "3.6.4.13" ], "uniref50_id": "UniRef50_P47664", "cog_id": "COG0513", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75172", "EC:3.6.4.13", "UniRef:UniRef50_P47664", "COG:COG0513", "COG:L", "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P47664", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47664" ], "product": "Probable ATP-dependent RNA helicase MG425" } }, "id": "CNOCLNGIAL_1316", "locus": "GCA000143945_01316" }, { "type": "cds", "sequence": "contig_1", "start": 745882, "stop": 746097, "strand": "+", "frame": 1, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "SO:0001217", "UniRef:UniRef50_Q6MTC5", "UniRef:UniRef90_P75171" ], "nt": "TTGTTACAATTATTTGATATGGCAAAAAAAGACCAACTTACTTTAAGAGGGCCTTTGTATGGCAACAATCGTTCCCACTCCAAGACTATTACGCGCAGAAAGTGGAATGTTAACCTCCAACCATGCAAGGTCAAAACTGCTGATGGCAAGACCACCAGAATCTTAGTTTCTACCAGAACACTGCGCACCTTAAAGAAACACAACCGCCTCTCTTAG", "aa": "MLQLFDMAKKDQLTLRGPLYGNNRSHSKTITRRKWNVNLQPCKVKTADGKTTRILVSTRTLRTLKKHNRLS", "aa_hexdigest": "74510e73d215b5087aac105bb3dc7cc6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75171", "query_cov": 0.9154929577464789, "subject_cov": 1.0, "identity": 1.0, "score": 127.0, "evalue": 4.1e-37, "valid": true, "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q6MTC5", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MTC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MTC5" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "CNOCLNGIAL_1317", "locus": "GCA000143945_01317" }, { "type": "cds", "sequence": "contig_1", "start": 746141, "stop": 746566, "strand": "+", "frame": 2, "gene": "yhfA", "product": "Hydroperoxide reductase", "db_xrefs": [ "COG:COG1765", "COG:R", "EC:1.11.1.-", "GO:0004601", "GO:0005737", "RefSeq:WP_010874982.1", "SO:0001217", "UniParc:UPI00001397C2", "UniRef:UniRef100_P75170", "UniRef:UniRef50_P47666", "UniRef:UniRef90_P75170" ], "nt": "ATGGACAAAAAGTACGACATCACTGCTGTTTTAAACGAAGACAGCTCCATGACTGCCATCAGTGATCAGTTTCAAATTACACTCGATGCCCGTCCCAAGCACACCGCTAAGGGTTTTGGTCCTTTAGCTGCTCTGCTCTCTGGTTTAGCTGCTTGTGAATTGGCAACCGCTAACTTGATGGCACCAGCTAAAATGATTACCATCAATAAACTGTTGATGAATGTCACTGGTTCACGCTCAACCAACCCCACCGATGGTTATTTTGGTTTAAGGGAAATTAACCTGCACTGGGAAATCCACTCCCCTAACAGTGAAACGGAAATCAAGGAGTTCATTGACTTTGTTTCCAAACGCTGCCCAGCGCACAATACGCTCCAAGGTGTGAGTCAACTCAAGATTAACGTTAATGTAACTTTAGTGCATTAA", "aa": "MDKKYDITAVLNEDSSMTAISDQFQITLDARPKHTAKGFGPLAALLSGLAACELATANLMAPAKMITINKLLMNVTGSRSTNPTDGYFGLREINLHWEIHSPNSETEIKEFIDFVSKRCPAHNTLQGVSQLKINVNVTLVH", "aa_hexdigest": "72cd36b4ae77fb22d150dbc14598aff0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001397C2", "ncbi_nrp_id": "WP_010874982.1", "uniref100_id": "UniRef100_P75170", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001397C2", "RefSeq:WP_010874982.1", "UniRef:UniRef100_P75170" ] }, "ips": { "uniref100_id": "UniRef100_P75170", "uniref90_id": "UniRef90_P75170" }, "psc": { "uniref90_id": "UniRef90_P75170", "gene": "YhfA", "product": "Hydroperoxide reductase", "ec_ids": [ "1.11.1.-" ], "uniref50_id": "UniRef50_P47666", "cog_id": "COG1765", "cog_category": "R", "go_ids": [ "GO:0005737", "GO:0004601" ] }, "pscc": { "uniref50_id": "UniRef50_P47666", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47666" ], "product": "Hydroperoxide reductase" }, "genes": [ "yhfA" ], "id": "CNOCLNGIAL_1318", "locus": "GCA000143945_01318" }, { "type": "cds", "sequence": "contig_1", "start": 746797, "stop": 746943, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAATTGCGCTCCACGCCGAGCATGACTCCAAGAAGCGCTATGACCCCAAATACGGTTTGAGCTTTGAGACCTACTTGAAGTTAAATGGTGCTAACTTCATTAAGTCGAGTTACCGTAGCTTAATGAATAAGGTTGATGACTGA", "aa": "MSIALHAEHDSKKRYDPKYGLSFETYLKLNGANFIKSSYRSLMNKVDD", "aa_hexdigest": "27e5f115f6e63c225c5d5c1df04044e8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5583.270300000002, "isoelectric_point": 9.049830055236818 }, "id": "CNOCLNGIAL_1319", "locus": "GCA000143945_01319" }, { "type": "cds", "sequence": "contig_1", "start": 746959, "stop": 747219, "strand": "+", "frame": 1, "gene": null, "product": "putative MG428-like protein", "db_xrefs": [ "GO:0003700", "GO:0006352", "SO:0001217", "UniRef:UniRef50_P47667", "UniRef:UniRef90_P75169" ], "nt": "ATGAAAACACTCGAGGAACAAAACACAGTCTCTTACACTCCTGAAAACTATTTAAGAAGTGTAGAATTCCGTGAAATGGTGCACTTAGCGTTTAAGAAAGCCAAGAATGAGGATGAACGTCAGGTGTTTGCGCTTTATGTAAAGGGTTACAAAAATTTAGAGATTGCTAAAAAACTCAACCTAACGACCCGTAGAGTGCGCTACCTAATTTCAATCTTTAAGGATCACATTAAGGTATTGACTAAACGGTATGGTTACTAG", "aa": "MKTLEEQNTVSYTPENYLRSVEFREMVHLAFKKAKNEDERQVFALYVKGYKNLEIAKKLNLTTRRVRYLISIFKDHIKVLTKRYGY", "aa_hexdigest": "239f6dca832476888c6b6e2744552adc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 746701, "stop": 747219, "observations": { "mutation": [ 746755, 746941 ], "stop": [ 746755, 746941 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75169", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 168.0, "evalue": 6.81e-52, "gene_start": 1, "gene_end": 86, "reference_start": 87, "reference_end": 172, "reference_sequence": "MKLQIFNTKLGLLAAKIYWKSWRHLGLSEDEIVSIALHAEHDSKKRYDPKYGLSFETYLKLNGANFIKSSYRSLMNKVDDWIGFKDMKTLEEQNTVSYTPENYLRSVEFREMVHLAFKKAKNEDERQVFALYVKGYKNLEIAKKLNLTTRRVRYLISIFKDHIKVLTKRYGY" }, "paralog": false, "identity": 0.9825581395348837, "score": 334.0, "evalue": 1.1e-119, "description": "Internal stop codon at 746755, 746941. Nonsense mutation around 746755, 746941.", "psc": { "uniref90_id": "UniRef90_P75169", "product": "Uncharacterized protein MG428 homolog", "uniref50_id": "UniRef50_P47667", "go_ids": [ "GO:0003700", "GO:0006352" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75169", "UniRef:UniRef50_P47667", "GO:0003700", "GO:0006352" ] }, "pscc": { "uniref50_id": "UniRef50_P47667", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47667" ], "product": "Uncharacterized protein MG428" } }, "id": "CNOCLNGIAL_1320", "locus": "GCA000143945_01320" }, { "type": "cds", "sequence": "contig_1", "start": 747216, "stop": 747446, "strand": "-", "frame": 1, "gene": null, "product": "PEP-utilising enzyme C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0K9GSR8" ], "nt": "ATGTGTGGTGAGATGGCCGGTGAACCGTTAGCGATTCCCCTACTTTTAGGTTTGGGTCTCAAAGAGTTTTCCATGAGTGCTAGTTCCATGTTTAAGGCGCGGATGATTATCGCTAAGCTCAATTACACAGAGTGTCAGACATTAGCACAAAAAGCATTAACCTTAGCGAACGCCAAGGAGGTGGAAAAGCTAGTGGAAAAATTCTTCAAGAAGAAGGATATCTTCATCTAG", "aa": "MCGEMAGEPLAIPLLLGLGLKEFSMSASSMFKARMIIAKLNYTECQTLAQKALTLANAKEVEKLVEKFFKKKDIFI", "aa_hexdigest": "34a06d14b9bb79439c625a730ce9b8d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6L3V2M9", "query_cov": 0.8552631578947368, "subject_cov": 0.9027777777777778, "identity": 0.569, "score": 69.3, "evalue": 4.51e-14, "valid": false, "product": "PEP-utilising enzyme C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A0K9GSR8" }, "pscc": { "uniref50_id": "UniRef50_A0A0K9GSR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0K9GSR8" ], "product": "PEP-utilising enzyme C-terminal domain-containing protein" }, "genes": [], "id": "CNOCLNGIAL_1321", "locus": "GCA000143945_01321" }, { "type": "cds", "sequence": "contig_1", "start": 747453, "stop": 747974, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "GTGTTAGAACAAGCGCACGGTGATTTAGTGATTATCCGCACCCTAGACATTGGTGGGGACAAGAAGCTTAATTACTATGAGTTTCCTCACGAAGATAACCCCTTCTTGGGCTACCGCGCTTTGCGTTTAACCTTAGACAAACAGGACATCTTTAAAACACAATTGCGGGCTTTACTACGTGCTGCTGATCACGGTCAGTTGGGCATTATGTTCCCGATGGTCGCTACCTTGGATGAACTGTTGCAAGCCAAGCAACTGTTAAACCAGGTGCACCAGGAGTTGGGTGGCAATAAACAGTTTAAGCTCGGGATTATGATCGAAATCCCCGCTGCTGTTTTAGCGGCTAACACCTTAAGTCACCACGTGGATTTCTTCTCCATTGGTACCAATGACCTGATCCAGTACAGCTTTGCGGCTGACCGGATGAATAAGAATGTTAGTTATCTCTACCAACCTTTAAACCCAGCTTTATTAAAACTCATTTACCTCACCATTGAAGGGGGTAAAGTGAATGATATTTGA", "aa": "MLEQAHGDLVIIRTLDIGGDKKLNYYEFPHEDNPFLGYRALRLTLDKQDIFKTQLRALLRAADHGQLGIMFPMVATLDELLQAKQLLNQVHQELGGNKQFKLGIMIEIPAAVLAANTLSHHVDFFSIGTNDLIQYSFAADRMNKNVSYLYQPLNPALLKLIYLTIEGGKVNDI", "aa_hexdigest": "0c7ee1613f3aa3f4bc83d4d45e76f441", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 174, "aa_cov": 0.976878612716763, "hmm_cov": 1.0, "evalue": 2.6881941909181362e-71, "score": 235.5618133544922, "start": 1, "stop": 169 } ], "seq_stats": { "molecular_weight": 19522.377600000003, "isoelectric_point": 6.038313102722167 }, "id": "CNOCLNGIAL_1322", "locus": "GCA000143945_01322" }, { "type": "cds", "sequence": "contig_1", "start": 748011, "stop": 748934, "strand": "-", "frame": 1, "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "db_xrefs": [ "COG:COG1080", "COG:G", "EC:2.7.3.9", "GO:0005737", "GO:0008965", "GO:0009401", "GO:0016301", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47668", "UniRef:UniRef90_P75168" ], "nt": "ATGAAAAAACTATCTGGTATCGGTGTATCTGATGGCATGGCCCTAGCCAAAGCCTTCCTTGTTAAAACACCGGAATTCGCTGTTAACAAATACCTCAAGCACCAATTAACGAAGGCGCAGGCCAAAAGACTCTTGGACTCAGCTTTCAAAAAAGCCGTTAAGGACTTAGAGGAAATTAAGGAAATTACCGTTAACAACATTAACACTGAAGCGGGCATGATCTTTGATGCGCACATTCAAATACTCAACGATCCCACGATCACCGAGCAACTAGAACAACAGTTAGCGCAAAATGTTCATCCTGTTATAGCCGTGGACACTGTCTTTAGCCAAACGGCTACCATGTTTAGCCAAATGCAGGACAAGTATTTCCAGGAACGTGCTGCAGACATTTTGGACTTGCGCCAACGCTTATTAGCTTATTTAACCGGACAAAAACCACATGACCTGGTCAAGATTAAGAGTGATGTCATTATTGTGGCCCATGACTTAACGCCGAGTCAAACTGCTACCCTTAACAAAAAATACGTCAAGGGTTTTTTAACCGAAATAGGTGGTCGTACCAGTCACGCTGCCATTATGGCGCGTTCACTGGAAATTCCCGCAGTGGTGGGCATTAAGGGCATTACTACTAAGGTCAAAGACGGTCAAATAGTTGGGGTTGATGGGCGCAAAGGAATAGCTGGCTTAGACCTAAACTCCAAGGACACTACCGAGTGGAAGAAACAAAAAGCGTTGGAAGAAAAATACCAACAAGAACTCAAGCAGTACACCAACAAGGAAACCGTGACTTTAGATGGTCATGCGGTTGTGGTGGCTGCTAACATTGGTAATGTGAAAGACATGGAATTGGCATGTCAGTACAATACCAACGGGGTTGGTTTATTCCGCACTGAGTTCCTCTACATGAACAGTCAAGAATGA", "aa": "MKKLSGIGVSDGMALAKAFLVKTPEFAVNKYLKHQLTKAQAKRLLDSAFKKAVKDLEEIKEITVNNINTEAGMIFDAHIQILNDPTITEQLEQQLAQNVHPVIAVDTVFSQTATMFSQMQDKYFQERAADILDLRQRLLAYLTGQKPHDLVKIKSDVIIVAHDLTPSQTATLNKKYVKGFLTEIGGRTSHAAIMARSLEIPAVVGIKGITTKVKDGQIVGVDGRKGIAGLDLNSKDTTEWKKQKALEEKYQQELKQYTNKETVTLDGHAVVVAANIGNVKDMELACQYNTNGVGLFRTEFLYMNSQE", "aa_hexdigest": "c1eb99295f1880dd5025e41e1a4dea03", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "ptsI" ], "truncated": "5-prime", "pseudogene": { "start": 747708, "stop": 748934, "observations": { "mutation": [ 748013 ], "stop": [ 748013 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75168", "query_cov": 1.0, "subject_cov": 0.5367132867132867, "identity": 0.997, "score": 587.0, "evalue": 3.02e-207, "gene_start": 1, "gene_end": 307, "reference_start": 1, "reference_end": 307, "reference_sequence": "MKKLSGIGVSDGMALAKAFLVKTPEFAVNKYLKHQLTKAQAKRLLDSAFKKAVKDLEEIKEITVNNINTEAGMIFDAHIQMLNDPTITEQLEQQLAQNVHPVIAVDTVFSQTATMFSQMQDKYFQERAADILDLRQRLLAYLTGQKPHDLVKIKSDVIIVAHDLTPSQTATLNKKYVKGFLTEIGGRTSHAAIMARSLEIPAVVGIKGITTKVKDGQIVGVDGRKGIAGLDLNSKDTTEWKKQKALEEKYQQELKQYTNKETVTLDGHAVVVAANIGNVKDMELACQYNTNGVGLFRTEFLYMNSQEWPDEETQYQAYKAVLEQAHGDLVIIRTLDIGGDKKLNYYEFPHEDNPFLGYRALRLTLDKQDIFKTQLRALLRAADHGQLGIMFPMVATLDELLQAKQLLNQVHQELGGNKQFKLGIMIEIPAAVLAANTLSHHVDFFSIGTNDLIQYSFAADRMNKNVSYLYQPLNPALLKLIYLTIEGGKVNDIWTGMCGEMAGEPLAIPLLLGLGLKEFSMSASSMFKARMIIAKLNYTECQTLAQKALTLANAKEVEKLVEKFFKKKDIFI" }, "paralog": false, "identity": 0.9950980392156863, "score": 794.0, "evalue": 3.34e-291, "description": "Internal stop codon at 748013. Nonsense mutation around 748013.", "psc": { "uniref90_id": "UniRef90_P75168", "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "ec_ids": [ "2.7.3.9" ], "uniref50_id": "UniRef50_P47668", "cog_id": "COG1080", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75168", "EC:2.7.3.9", "UniRef:UniRef50_P47668", "COG:COG1080", "COG:G", "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47668", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47668" ], "product": "Phosphoenolpyruvate-protein phosphotransferase" } }, "id": "CNOCLNGIAL_1323", "locus": "GCA000143945_01323" }, { "type": "cds", "sequence": "contig_1", "start": 748939, "stop": 750465, "strand": "-", "frame": 3, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0016020", "GO:0030145", "GO:0046537", "GO:0051919", "KEGG:K15633", "RefSeq:WP_014325644.1", "SO:0001217", "UniParc:UPI0001DD645C", "UniRef:UniRef100_A0AAX0SPX1", "UniRef:UniRef50_P75167", "UniRef:UniRef90_P75167" ], "nt": "ATGCATAAGAAGGTCTTACTAGCCATTTTGGATGGTTATGGTATTAGTAATAAGCAACACGGTAATGCGGTGTACCATGCTAAAACTCCAGCTTTAGACAGTTTGATTAAGGATTATCCCTGTGTCATGTTAGAAGCTTCGGGGGAAGCCGTTGGTCTACCACAAGGACAAATCGGTAACTCGGAAGTGGGTCACCTCAATATTGGGGCTGGTCGAATTGTTTACACTGGTTTATCGTTAATTAACCAAAACATTAAAACCGGGGCCTTTCACCACAACCAGGTGTTACTAGAGGCGATTGCTAGGGCCAAGGCTAACAATGCTAAGTTGCACTTAATTGGTCTGTTTTCCCATGGCGGGGTACACAGTCACATGGACCATTTGTATGCCTTGATTAAACTAGCAGCACCGCAGGTCAAAATGGTGCTCCACCTCTTTGGTGATGGGCGGGATGTAGCACCTTGCACGATGAAGAGTGACCTTGAAGCCTTCATGGTGTTCTTAAAGGATTACCACAATGTCATCATTGGTACCTTAGGTGGGCGTTACTATGGTATGGATCGTGACCAGCGCTGGGACAGAGAAGAGATTGCTTATAATGCCATTTTGGGTAACAGTAAGGCTAGCTTTACTGATCCGGTAGCTTATGTTCAAAGTGCCTATGACCAAAAGGTAACCGATGAGTTTTTGTACCCGGCTGTCAATGGTAATGTAGATAAAGAGCAGTTTGCGCTCAAAGATCATGACAGTGTGATCTTCTTTAACTTCCGTCCTGATCGTGCGCGGCAAATGTCACACATGCTCTTTCAAACAGATTACTATGACTACACCCCTAAAGCAGGACGAAAGCACAACCTCTTCTTTGTCACGATGATGAACTATGAGGGGATTAAACCAAGTGCCGTGGTCTTTCCGCCCGAAACTATTCCCAATACTTTTGGGGAAGTCATTGCGCACAATAAGTTAAAGCAACTGCGAATTGCAGAAACGGAAAAGTACGCCCATGTAACCTTCTTCTTTGATGGTGGAGTGGAAGTGGATCTACCCAATGAAACTAAGTGCATGGTACCTTCGTTAAAGGTGGCTACGTATGACTTAGCACCGGAAATGGCATGTAAGGGGATAACTGATCAATTACTAAATCAAATTAACCAGTTCGATTTGACGGTGTTGAACTTTGCCAACCCTGATATGGTAGGTCATACTGGTAACTATGCCGCTTGTGTGCAGGGCTTAGAGGCCTTGGATGTGCAAATACAGCGCATTATTGATTTCTGCAAGGCTAACCACATTACCCTGTTCTTAACTGCTGACCATGGTAACGCGGAAGAGATGATTGACAGCAACAATAACCCAGTTACCAAACACACTGTCAACAAGGTGCCGTTTGTATGTACTGATACAAACATTGACTTACAACAAGACAGTGCTAGTCTAGCCAATATTGCCCCAACCATTTTGGCTTACTTAGGGCTCAAACAGCCCGCAGAAATGACCGCAAACTCCCTTTTAATAAGTAAAAAGTAA", "aa": "MHKKVLLAILDGYGISNKQHGNAVYHAKTPALDSLIKDYPCVMLEASGEAVGLPQGQIGNSEVGHLNIGAGRIVYTGLSLINQNIKTGAFHHNQVLLEAIARAKANNAKLHLIGLFSHGGVHSHMDHLYALIKLAAPQVKMVLHLFGDGRDVAPCTMKSDLEAFMVFLKDYHNVIIGTLGGRYYGMDRDQRWDREEIAYNAILGNSKASFTDPVAYVQSAYDQKVTDEFLYPAVNGNVDKEQFALKDHDSVIFFNFRPDRARQMSHMLFQTDYYDYTPKAGRKHNLFFVTMMNYEGIKPSAVVFPPETIPNTFGEVIAHNKLKQLRIAETEKYAHVTFFFDGGVEVDLPNETKCMVPSLKVATYDLAPEMACKGITDQLLNQINQFDLTVLNFANPDMVGHTGNYAACVQGLEALDVQIQRIIDFCKANHITLFLTADHGNAEEMIDSNNNPVTKHTVNKVPFVCTDTNIDLQQDSASLANIAPTILAYLGLKQPAEMTANSLLISKK", "aa_hexdigest": "0c31da24fa2b730de551030ec80f6730", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD645C", "ncbi_nrp_id": "WP_014325644.1", "uniref100_id": "UniRef100_A0AAX0SPX1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD645C", "RefSeq:WP_014325644.1", "UniRef:UniRef100_A0AAX0SPX1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SPX1", "uniref90_id": "UniRef90_P75167" }, "psc": { "uniref90_id": "UniRef90_P75167", "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_P75167", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0006007", "GO:0006096", "GO:0016020", "GO:0030145", "GO:0046537", "GO:0051919" ] }, "pscc": { "uniref50_id": "UniRef50_P75167", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75167" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "CNOCLNGIAL_1324", "locus": "GCA000143945_01324" }, { "type": "cds", "sequence": "contig_1", "start": 750458, "stop": 750640, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00121.24" ], "nt": "GTGTACCTTAGTGAACTGTACAACAAGGAAATTGCCCAACAAACCTCGATTCTCTATGGTGGTTCGGTTGCTAAGGACAACATTAAGGAGTTAGCACAAACCGAGCAAATTGATGGCTTTTTAGTGGGCAAGGCATCTTTGGATGTCAACGACTTTTTAGTAATGGCACAAGTATATGCATAA", "aa": "MYLSELYNKEIAQQTSILYGGSVAKDNIKELAQTEQIDGFLVGKASLDVNDFLVMAQVYA", "aa_hexdigest": "d51d19e6c8eaf0e8e0101c3f8fe5ec8f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00121.24", "name": "TIM", "length": 55, "aa_cov": 0.9166666666666666, "hmm_cov": 0.9818181818181818, "evalue": 6.923689029538065e-18, "score": 60.47081756591797, "start": 3, "stop": 57 } ], "seq_stats": { "molecular_weight": 6645.474500000002, "isoelectric_point": 4.285114479064942 }, "id": "CNOCLNGIAL_1325", "locus": "GCA000143945_01325" }, { "type": "cds", "sequence": "contig_1", "start": 750704, "stop": 751126, "strand": "-", "frame": 2, "gene": "tpiA", "product": "triose-phosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_P78010", "UniRef:UniRef90_P78010" ], "nt": "GTGGAGCAATTTAAACAGCACCCTGCTAAAACTAAGGCGGTTTTGGGTATTGCCCCAGTCCACGTCCACTTAACCGAGGTAAACAAAGTCTTGCCTAATAACCTCTTACTGTTAGCCCAAGACGCTAACTTTATTGCTAGTGGTTCTTACACTGGTACGGTGAGCTATACCCAACTGCAAGACATTAAGGTCAACAGTGTCATTATTGGTCACTCGGAACGGCGAAAGTACTTTAACGAAACAGCGCAAGTTATTAATCAAAAACTAAAAGCCTGTTTACAAGCTGGAATGTTAGTCGTGCTCTGTATTGGTGAAACGGAAGGTCAACCAATTAGTTTTCTCAAAGAAGACTTAACCCAAGTCTTGCAAGGCATTGACCTAAGCTTACTCAAACAGTTAGTGATTGCTTACGAACCAATTTGA", "aa": "MEQFKQHPAKTKAVLGIAPVHVHLTEVNKVLPNNLLLLAQDANFIASGSYTGTVSYTQLQDIKVNSVIIGHSERRKYFNETAQVINQKLKACLQAGMLVVLCIGETEGQPISFLKEDLTQVLQGIDLSLLKQLVIAYEPI", "aa_hexdigest": "9c5023fcb743242a885b5d55a7d0cbe1", "start_type": "GTG", "rbs_motif": null, "genes": [ "tpiA" ], "truncated": "both", "pseudogene": { "start": 750458, "stop": 751192, "observations": { "mutation": [ 750706, 751165 ], "stop": [ 750706, 751165 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78010", "query_cov": 1.0, "subject_cov": 0.5737704918032787, "identity": 0.993, "score": 271.0, "evalue": 1.55e-90, "gene_start": 1, "gene_end": 140, "reference_start": 23, "reference_end": 162, "reference_sequence": "MRTKYLIGNWKTNKDLHQALAFVEQFKQHPAKTKAVLGIAPVHVHLTEVNKVLPNNLLLLAQDANFIASGSYTGTVSYTQLQDIKVNSVIIGHSERRKYFNETAQVINQKLKACLQAGMLVVLCIGETEGQPISFLKEDLTQVLQGIDLSLLKQLVIAYEPIWAIGTGKTATPEIANNTIAQIRVYLSELYNKEIAQQTSILYGGSVAKDNIKELAQTEQIDGFLVGKASLDVNDFLVMAQVYA" }, "paralog": false, "identity": 0.9918032786885246, "score": 466.0, "evalue": 1.4e-169, "description": "Internal stop codon at 750706, 751165. Nonsense mutation around 750706, 751165.", "psc": { "uniref90_id": "UniRef90_P78010", "gene": "tpiA", "product": "triose-phosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_P78010", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78010", "EC:5.3.1.1", "UniRef:UniRef50_P78010", "COG:COG0149", "COG:G", "KEGG:K01803", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ] }, "pscc": { "uniref50_id": "UniRef50_P78010", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78010" ], "product": "Triosephosphate isomerase" } }, "id": "CNOCLNGIAL_1326", "locus": "GCA000143945_01326" }, { "type": "cds", "sequence": "contig_1", "start": 751245, "stop": 752459, "strand": "-", "frame": 1, "gene": "yitT", "product": "putative MG432-like protein", "db_xrefs": [ "COG:COG1284", "COG:S", "GO:0005886", "SO:0001217", "UniParc:UPI0001DD645D", "UniRef:UniRef100_A0A0H3DM54", "UniRef:UniRef50_Q49432", "UniRef:UniRef90_P75166" ], "nt": "ATGCAAGATAAAAACGTCAAAATTCAGGGCAATCTGGTACGGGTACACCTTTCGGGATCGTTTCTGAAGTTCCAGGCAATTTACAAGGTGAAAAAGCTGTACTTACAGCTGTTAATTCTCTCCGTGATTGCCTTCTTTTGGGGCTTGTTAGGGGTTGTGTTTGTCCAGTTTTCTGGATTATATGACATTGGCATTGCTTCCATTAGTCAGGGCTTAGCACGGTTAGCGAATTATTTAATTAGGTCGAACAAGGTCAGTGTGGATGCTGACACCATTTACAACGTCATCTTCTGGTTGAGTCAAATTCTGATTAACATTCCCTTATTTGTTTTGGGTTGGTACAAGATTTCCAAAAAGTTTACCTTGTTAACCCTTTACTTTGTGGTAGTCTCCAACGTTTTTGGGTTTGCCTTCTCTTACATTCCGGGCGTGGAAAACTTCTTCTTGTTTGCTAATTTAACTGAACTTACTAAGGCCAACGGTGGCTTAGAACAAGCGATTAACAACCAAGGGGTGCAACTGATCTTTTGGGAACAAACCGCTGAAAAGCAAATTTCGTTAATGTTCTATGCGCTGATCTGGGGTTTTCTTCAAGCTGTGTTTTACTCAGTTATCCTAATTATTGATGCATCGAGTGGTGGGTTGGACTTTTTGGCCTTCTGGTATTCGGAAAAGAAACACAAGGACATTGGTGGTATTTTGTTTATTGTTAACACCCTTAGTTTCTTGATCGGTTACACCATTGGCACTTACCTTACCGGTAGCTTACTAGCACAAGGCTTTCAAGGAGATAGACAAAAACCGTTTGGAGTGGCTTTTTTCTTGTCCCCTAACTTAGTGTTTACGATCTTCATGAACATTATCTTAGGGATCTTTACCTCCTACTTCTTTCCTAAATACCAGTTTGTCAAAGTGGAAGTGTATGGTAAACACATGGAACAAATGCGCAACTACTTGTTGAGCAGTAACCAGTCCTTTGCGGTCACTATGTTCGAAGTGGAAGGGGGGTATTCGCGCCAAAAGAACCAGGTGTTAGTTACAAACTGTTTGTTTACGAAAACGGCCGAACTTTTAGAAGCTGTTAGACGAGTCGATCCGGATGCTCTGTTCTCAATTACCTTCATTAAAAAGTTGGATGGTTATATCTATGAAAGAAAAGCACCTGATAAAGTAGTCCCACCAGTAAAAGACCCAGTTAAAGCCCAGGAAAATTAA", "aa": "MQDKNVKIQGNLVRVHLSGSFLKFQAIYKVKKLYLQLLILSVIAFFWGLLGVVFVQFSGLYDIGIASISQGLARLANYLIRSNKVSVDADTIYNVIFWLSQILINIPLFVLGWYKISKKFTLLTLYFVVVSNVFGFAFSYIPGVENFFLFANLTELTKANGGLEQAINNQGVQLIFWEQTAEKQISLMFYALIWGFLQAVFYSVILIIDASSGGLDFLAFWYSEKKHKDIGGILFIVNTLSFLIGYTIGTYLTGSLLAQGFQGDRQKPFGVAFFLSPNLVFTIFMNIILGIFTSYFFPKYQFVKVEVYGKHMEQMRNYLLSSNQSFAVTMFEVEGGYSRQKNQVLVTNCLFTKTAELLEAVRRVDPDALFSITFIKKLDGYIYERKAPDKVVPPVKDPVKAQEN", "aa_hexdigest": "cbc9af767cb9caa3b0e8497946ab47de", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0001DD645D", "uniref100_id": "UniRef100_A0A0H3DM54", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD645D", "UniRef:UniRef100_A0A0H3DM54" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DM54", "uniref90_id": "UniRef90_P75166" }, "psc": { "uniref90_id": "UniRef90_P75166", "gene": "YitT", "product": "Uncharacterized protein MG432 homolog", "uniref50_id": "UniRef50_Q49432", "cog_id": "COG1284", "cog_category": "S", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q49432", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49432" ], "product": "Uncharacterized protein MG432" }, "genes": [ "yitT" ], "id": "CNOCLNGIAL_1327", "locus": "GCA000143945_01327" }, { "type": "cds", "sequence": "contig_1", "start": 752546, "stop": 752662, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACAAAAATAGAGCTCATAAAAGAACTACGTAAAACAACACAAGCGAGCATGATGGACTGTAAAAAGGCTTTGGAACAAAATAACGATGATCTCGAAAAGGCCATTAAGTGA", "aa": "MATKIELIKELRKTTQASMMDCKKALEQNNDDLEKAIK", "aa_hexdigest": "f0cb3a4a31ad7258ac8d1163fc305f1b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4381.1236, "isoelectric_point": 7.870574378967285 }, "id": "CNOCLNGIAL_1328", "locus": "GCA000143945_01328" }, { "type": "cds", "sequence": "contig_1", "start": 752663, "stop": 753442, "strand": "+", "frame": 2, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "SO:0001217", "UniRef:UniRef50_P47246", "UniRef:UniRef90_P78009" ], "nt": "TTGAGAGAAAACGGAATTGTTAAATCTGCTAAGAAATTAGGCAAAGTAGCCGCTGATGGTTGCATTGTGTTACACAGTGATCACCACAAGGCAGTGATGGTGGAAATTAATTCTCAAACGGACTTTGTAGCGCGTAGTCAAGAGTTAACGGACTTTGCGCAATTAATGATTAGTGAAGTCTTTAAAAAGGCTACCCCTACCACTACTATTGAAGAAGTAACCCAGTACGAACTCCAAGGCAAAGAAAAGGTAGCAGAACGTTTGGCTTTAGTCGCTTCCAAAACTGATGAAAAGATTGTGTTGCGCCGCTTAATGGTGTTTGAAAGCAAAACTAACCACATCTTTAGCTACCTCCACGCTAACAAACGGATTGGCGTAATCTTGGAAGTGGAAGGCAAGTTTGATGAACAAGATGGTAAACACTTAGCAATGCACATTGCCGCTAACTCACCGCAGTTCATTGATCAGGACAATGTGGATCAAACATGGTTAGCTAACGAAACCAGCATCATTAAGTCCCAAGCCAAACTAGAAGTCCAAGACAATCCGAAGAAAGCAGCGTTCTTAGACAAAACTATTGCTGGACGCGTGAACAAACTCTTAATTGACATTTGTCTGGTAAACCAAAAGTACTTGGTCGATGAATCCAAAACAGTTGGTCAATTCTTAAAGGAAAAGAACAGTAAAGTAATCCATTTTGTGCGCTTTGAAGTTGGTGAAGGCATTGTCAAAGAAGCAGTTGACTTCGCTAGTGAAGTAAGTGCGCAAATGAAAAAATAA", "aa": "MRENGIVKSAKKLGKVAADGCIVLHSDHHKAVMVEINSQTDFVARSQELTDFAQLMISEVFKKATPTTTIEEVTQYELQGKEKVAERLALVASKTDEKIVLRRLMVFESKTNHIFSYLHANKRIGVILEVEGKFDEQDGKHLAMHIAANSPQFIDQDNVDQTWLANETSIIKSQAKLEVQDNPKKAAFLDKTIAGRVNKLLIDICLVNQKYLVDESKTVGQFLKEKNSKVIHFVRFEVGEGIVKEAVDFASEVSAQMKK", "aa_hexdigest": "bdf2184fb757c8bf8769a32d2c5e8e8d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P78009", "query_cov": 1.0, "subject_cov": 0.8691275167785235, "identity": 0.996, "score": 496.0, "evalue": 2.1e-176, "valid": true, "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_P47246", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_P47246", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47246" ], "product": "Elongation factor Ts" }, "genes": [ "tsf" ], "id": "CNOCLNGIAL_1329", "locus": "GCA000143945_01329" }, { "type": "cds", "sequence": "contig_1", "start": 753444, "stop": 754151, "strand": "+", "frame": 3, "gene": "pyrH", "product": "UMP kinase", "db_xrefs": [ "COG:COG0528", "COG:F", "EC:2.7.4.22", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210", "KEGG:K09903", "SO:0001217", "UniParc:UPI0001DD6475", "UniRef:UniRef100_A0AAN4XGQ3", "UniRef:UniRef50_P47672", "UniRef:UniRef90_P75165" ], "nt": "ATGCGCCTAAAGATCCTAATCAAACTAAGCGGTGCTGGCATGAGCGCTGATAGTTCCGAACCGTTTTCCAACCAATTTTTAGAAACTGTCATCGCTCAACTAAAACAACTAGTACCCAACTACCAAATAGGCATTGTGATTGGTGGGGGCAACATTATGCGCGGTAAGTCTTGTAGTGACTACAACATTACCGAAATAGCAGGGCACCACTTGGGCATTATGGCCACGGTTATTAACGGCGCTTTTTTAAAGGCTAAGTTTGATAGTCATAAACTTAACAGCACCCTGTTGAGTGCAGTGAGCTGTCCTAGTTTAGCGACCCACATTGTGTCCCAGGCCACGATTGATGATGCCTTTAAAAACCATGACATTGTCATTTTTGCGGGCGGTACTGGTAACCCGTACTTTTCGACTGATACCGCTGTGGCGTTGCGTGCTACCCAAATGCAAGCCGATATTATTCTAATTGGCAAGAATGGTGTTGATGGGGTTTACACTGCGGATCCTAAAAAGGATAAGCATGCTAAGTTTTTAGCTAGCTTAACTTACGCTGAAGCGATTAAGAATGACCTGCAAATCATGGACATTACCGCCTTTACAATGTGTAAGGAAAATAACTTAAAGGTGATCATATTTAACATCAATGCCGAGCATGCCATCATTAAGGCATTGAGCAAACAAGGTAAATACACCTTAATAGAAAAATAA", "aa": "MRLKILIKLSGAGMSADSSEPFSNQFLETVIAQLKQLVPNYQIGIVIGGGNIMRGKSCSDYNITEIAGHHLGIMATVINGAFLKAKFDSHKLNSTLLSAVSCPSLATHIVSQATIDDAFKNHDIVIFAGGTGNPYFSTDTAVALRATQMQADIILIGKNGVDGVYTADPKKDKHAKFLASLTYAEAIKNDLQIMDITAFTMCKENNLKVIIFNINAEHAIIKALSKQGKYTLIEK", "aa_hexdigest": "510b04b8c0c148efd9efe0ac5d887d14", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6475", "uniref100_id": "UniRef100_A0AAN4XGQ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6475", "UniRef:UniRef100_A0AAN4XGQ3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAN4XGQ3", "uniref90_id": "UniRef90_P75165" }, "psc": { "uniref90_id": "UniRef90_P75165", "gene": "pyrH", "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_P47672", "cog_id": "COG0528", "cog_category": "F", "kegg_orthology_id": "K09903", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ] }, "pscc": { "uniref50_id": "UniRef50_P47672", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47672" ], "product": "Uridylate kinase" }, "genes": [ "pyrH" ], "id": "CNOCLNGIAL_1330", "locus": "GCA000143945_01330" }, { "type": "cds", "sequence": "contig_1", "start": 754323, "stop": 755108, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4BAEE8", "UniRef:UniRef90_UPI000A4BAEE8" ], "nt": "TTGATTGCCAATGCGATTGATGAACACCGCTTAAGCAACACCGCTTTCCCCGTAATTGAATTGCAAAAAGGCTTTCTAAACTCTTCTTTAGTGATTAAAGATTATGGTAGGGGTATTAAGTCCAATCACTTTATCCAAAACGAAAGTAGGGAAAAGGTTCAAAGTGAAAAAACTATTGGTAAGTTTGGCATTGGTCTCAAAGATGCCATAGCTGTTCTTTTCCGTCATAATGTCAAAGTTAGCTTTACCAGTAGTGAAGGCACTTTTACCCCTGTTGAGCGAATGAAGGAAGGTATGAAAGACGGCACCAAAACAATTCAAATAACGGTGGATGAAACCAAGAAGATTGACAAGGGCACTGACATCCTCATTAGCAAGATTAGTAGAAGTGATTACGAAAAAGCCATTGCTATTTTTTTGGAATTAAGAACTGGCTATCAAAAACTGGCTTCTTCCAAAAAGGGTGATATTTATCGCAGTGAAAATGGTTCAGAGATCTTTCTTAACGGGATGAAAATCGGAACCGATGAAAACTTCTTGTTTTCTTATGACATTAAAGAACCCAATAAGAAATTGCAAAAATCCTTAAACCGGGAGCGTAAAACACTTTCCCGTGATAGCTACCGTGACAATATCATTTCCATTTTGAAGTCCAGCATTAATAAAAATACCCAAACTTTAATTAACCAATTAATTGACAACCGCGATCAGTTTGAAACTGGTGAATGGAGTTTTATTGATGTCAAAAAGTTAGTAATGCAAAACACTAACCGAAAGATTTTGTGA", "aa": "MIANAIDEHRLSNTAFPVIELQKGFLNSSLVIKDYGRGIKSNHFIQNESREKVQSEKTIGKFGIGLKDAIAVLFRHNVKVSFTSSEGTFTPVERMKEGMKDGTKTIQITVDETKKIDKGTDILISKISRSDYEKAIAIFLELRTGYQKLASSKKGDIYRSENGSEIFLNGMKIGTDENFLFSYDIKEPNKKLQKSLNRERKTLSRDSYRDNIISILKSSINKNTQTLINQLIDNRDQFETGEWSFIDVKKLVMQNTNRKIL", "aa_hexdigest": "9c79440ad272f34732688b266ca1f096", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A4BAEE8", "query_cov": 0.9272030651340997, "subject_cov": 0.8642857142857143, "identity": 0.992, "score": 458.0, "evalue": 1.01e-161, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_UPI000A4BAEE8" }, "pscc": { "uniref50_id": "UniRef50_UPI000A4BAEE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4BAEE8" ], "product": "ATP-binding protein" }, "genes": [], "id": "CNOCLNGIAL_1331", "locus": "GCA000143945_01331" }, { "type": "cds", "sequence": "contig_1", "start": 755211, "stop": 755375, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATGATGCCGAATTTAAAAGCTATACCCTCAACGATTTTGGTGATAGATTTGTCAATGAATTCCAAACGGAAGAGGTGCCATTCCACAAATTAACTGAGATCGAAAAAGACAACTGGAATTGGGTAATGGCTAAGGTCAAAGAACTTACCAGGGTTTGA", "aa": "MENDAEFKSYTLNDFGDRFVNEFQTEEVPFHKLTEIEKDNWNWVMAKVKELTRV", "aa_hexdigest": "65a8f1754f43d7a31f7033ce0dddd929", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6570.2459, "isoelectric_point": 4.637345695495605 }, "id": "CNOCLNGIAL_1332", "locus": "GCA000143945_01332" }, { "type": "cds", "sequence": "contig_1", "start": 755791, "stop": 755892, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00035C1894", "UniRef:UniRef100_A0AB38W6X6", "UniRef:UniRef50_A0AB38W6X6", "UniRef:UniRef90_A0AB38W6X6" ], "nt": "ATGTTTTATCTCGATTTAAACATTCAAAATATTCTTACCAATTGGGAGATAGCTGATGCTGTTCGCGAACTGATCGCTAACGCCATTGATGAGCACATTTAA", "aa": "MFYLDLNIQNILTNWEIADAVRELIANAIDEHI", "aa_hexdigest": "be05d12b720a62bb5b8bcb4ad1f3b971", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00035C1894", "uniref100_id": "UniRef100_A0AB38W6X6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00035C1894", "UniRef:UniRef100_A0AB38W6X6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6X6", "uniref90_id": "UniRef90_A0AB38W6X6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6X6", "uniref50_id": "UniRef50_A0AB38W6X6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6X6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6X6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3872.3594999999987, "isoelectric_point": 4.0500284194946286 }, "id": "CNOCLNGIAL_1333", "locus": "GCA000143945_01333" }, { "type": "cds", "sequence": "contig_1", "start": 756096, "stop": 756653, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SSK4", "UniRef:UniRef90_A0AAX0SSK4" ], "nt": "GTGAGTTTTACAAGTCAGCAAGGTACTTTTATTCCTGTAGAACGGAGTAAAACTGAACTGGAAGGTAGCATCAAAACAATTCAAATGGAAGTTGATCCAGCTGAACAAATTGAAAAAGCGGGTACCTTAATCACAATTGAAGGATTGAAAAAAGATGACTATGACAAAGCTATTGTTAATTTCTTAGCTCTACGGGAAGATTTGCAATTATTAGCTGCTTCACCCAAAGGCGATGTTTACCGTAATACATCAGGTAACGGCGCAGAAATTTTTCTCAACGGGATGAAAATTGCAACCGATGAAGACTTTCTCTTTTCCTATCACATTAAAGAACCTAACAAAAAGTTACAGCGTTCCCTTAACCGGGAAAATAAAAATTTACCAAGAGATTGTTATCGTGAAAACATCATCACCATTCTTAAGTCCAACATTAACAATAGAACACAAACACTTATTGATGAATTAATAGATTCACGTGACCAATATGACAATGGCGAGTGGAGTTTTATTGATGTCAAAAAACTAATTGGTTTAAACACAAACAGAAATATTTTGTGA", "aa": "MSFTSQQGTFIPVERSKTELEGSIKTIQMEVDPAEQIEKAGTLITIEGLKKDDYDKAIVNFLALREDLQLLAASPKGDVYRNTSGNGAEIFLNGMKIATDEDFLFSYHIKEPNKKLQRSLNRENKNLPRDCYRENIITILKSNINNRTQTLIDELIDSRDQYDNGEWSFIDVKKLIGLNTNRNIL", "aa_hexdigest": "7fbf0fa706b5bb500aed686f813f1f3d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAX0SSK4", "query_cov": 1.0, "subject_cov": 0.9296482412060302, "identity": 1.0, "score": 358.0, "evalue": 7.67e-125, "valid": true, "uniref50_id": "UniRef50_A0AAX0SSK4" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SSK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SSK4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21158.681400000016, "isoelectric_point": 5.207952308654785 }, "id": "CNOCLNGIAL_1334", "locus": "GCA000143945_01334" }, { "type": "cds", "sequence": "contig_1", "start": 756705, "stop": 756944, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACCAAAAAATATGAAATTTTGGCGTTAAATTCCTTGCAATACAGAAGCATGGAAAACGATGATCGTTTAAAAAAGCAAACACTGATGCATGTAAGTGAAAAGTTAAAACAACAAAGAATTGAAGAAGAGGCTGAGAAAATCAAAGTTAAAGAACAAAAGCCACGAAAACGATTTGAGGAAGAAGATTTGCCAATAGAAGATTTAAATCCAATTGAAAGAGAAGGTTGGGATTGA", "aa": "MDTKKYEILALNSLQYRSMENDDRLKKQTLMHVSEKLKQQRIEEEAEKIKVKEQKPRKRFEEEDLPIEDLNPIEREGWD", "aa_hexdigest": "50ffb4e4359e1d44410c4673845b3261", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9659.859599999996, "isoelectric_point": 5.302191352844238 }, "id": "CNOCLNGIAL_1335", "locus": "GCA000143945_01335" }, { "type": "cds", "sequence": "contig_1", "start": 756948, "stop": 757238, "strand": "+", "frame": 3, "gene": null, "product": "SprT-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S5E5", "UniRef:UniRef90_A0AAX0S5E5" ], "nt": "ATGGAAAAGGCCAGAGAATTATGTGGTTTTATAAGAGGATGGGAAAAACTTTATGAAGAATATCAGTTTGTTCTAATGGAAAAGAATCACAAATATGTCGGGCTTTGTTACACCGATCAAAAAATTATCAAACTCTCAAGAGAAATATTGAAAGATGAATACAGTTTATTGAATACCTTAGTGCATGAAATTTGTCATGCAACAACTAATGGTCGAGACGGAACGAAGAAGTTTGAAAGGGGTCTAACTGATGCTTTTCATCCATTATTTAAACTAGGGCAAAGTAAATAA", "aa": "MEKARELCGFIRGWEKLYEEYQFVLMEKNHKYVGLCYTDQKIIKLSREILKDEYSLLNTLVHEICHATTNGRDGTKKFERGLTDAFHPLFKLGQSK", "aa_hexdigest": "1911e99f4110e194e22ba1ec1d261d48", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 756654, "stop": 757238, "observations": { "mutation": [ 756693, 756942 ], "stop": [ 756693, 756942 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0S5E5", "query_cov": 1.0, "subject_cov": 0.4485981308411215, "identity": 0.99, "score": 202.0, "evalue": 1.6e-64, "gene_start": 1, "gene_end": 96, "reference_start": 119, "reference_end": 214, "reference_sequence": "MEFYDVKKLIGLNTNRNILWADSSSKNIEKLIYSLYGMDTKKYEILALNSLQYRSMENDDRLKKQTLMHVSEKLKQQRIEEEAEKIKVKEQKPRKRFEEEDLPIEDLNPIEREGWDWAMEKARELCGFIRGWEKLYEEYQFVLMEKNHKYVGLCYTDQKIIKLSRGILKDEYSLLNTLVHEICHATTNGRDGTKKFERGLTDAFHPLFKLGQSK" }, "paralog": false, "identity": 0.9845360824742269, "score": 385.0, "evalue": 8.17e-139, "description": "Internal stop codon at 756693, 756942. Nonsense mutation around 756693, 756942.", "psc": { "uniref90_id": "UniRef90_A0AAX0S5E5", "product": "SprT-like domain-containing protein", "uniref50_id": "UniRef50_A0AAX0S5E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0S5E5", "UniRef:UniRef50_A0AAX0S5E5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAX0S5E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S5E5" ], "product": "SprT-like domain-containing protein" } }, "id": "CNOCLNGIAL_1336", "locus": "GCA000143945_01336" }, { "type": "cds", "sequence": "contig_1", "start": 757387, "stop": 757869, "strand": "+", "frame": 1, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023", "SO:0001217", "UniRef:UniRef50_P47673", "UniRef:UniRef90_P75161" ], "nt": "TTGAGCAAAATCCGTACTGGTAGACCTAATCCAAAACTGTTTGATAATATCCTTGTGGAAAGCTATGGTGACAGAATGCCAATGGTAGCATTAGCACAAATTGCGGTAAATCCACCGCGGGAAATTGTGATCAAGCCGTTTGACGTTAAAAACAATATTAATGCCATCTACAGCGAAATTCAACGTGCTAACTTAGGTGTACAACCAGTGATTGACGGAGATAAAATTCGCATTAACTTTCCCCCAATGACCCAAGAAAGTCGTTTGGAAAGCATTAAGCAAGCTAAAAAAGTAGTGGAACAAATCCACCAAGAGTTGCGCAGTGTGCGACGTGATACCCTTCAAATGATTAAGAAGGATGATCATAAAGACGAAGACTTTGAAGAATTCTTAAAAGAAGAAGTAGAAAAAGTTAACAAGCAGTATATTGCACAGTTAGAAACTATTCAAAAACAAAAAGAAAAAGAACTATTAGTGGTCTAA", "aa": "MSKIRTGRPNPKLFDNILVESYGDRMPMVALAQIAVNPPREIVIKPFDVKNNINAIYSEIQRANLGVQPVIDGDKIRINFPPMTQESRLESIKQAKKVVEQIHQELRSVRRDTLQMIKKDDHKDEDFEEFLKEEVEKVNKQYIAQLETIQKQKEKELLVV", "aa_hexdigest": "707cd85388fc8f405b301a1785ff56b9", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75161", "query_cov": 1.0, "subject_cov": 0.8648648648648649, "identity": 0.9940000000000001, "score": 299.0, "evalue": 5.07e-102, "valid": true, "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_P47673", "cog_id": "COG0233", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ] }, "pscc": { "uniref50_id": "UniRef50_P47673", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47673" ], "product": "Ribosome-recycling factor" }, "genes": [ "frr" ], "id": "CNOCLNGIAL_1337", "locus": "GCA000143945_01337" }, { "type": "cds", "sequence": "contig_1", "start": 757869, "stop": 757985, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCTGAAAGATAATTCCTTCGCTAAAAAACGATCGACAGTGTTCGTTGTTTTGCTCATTGTCTTTTGTTTTTTTTTAATGTTTTCAGCCTTTGCTGACGGCTTTAACTTTTGA", "aa": "MDLKDNSFAKKRSTVFVVLLIVFCFFLMFSAFADGFNF", "aa_hexdigest": "630807abe7f3f7807ea1454ee89a90fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4417.217199999999, "isoelectric_point": 7.933946800231935 }, "id": "CNOCLNGIAL_1338", "locus": "GCA000143945_01338" }, { "type": "cds", "sequence": "contig_1", "start": 758073, "stop": 758282, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATCCTGACTTTAAGGCAGTCCGTTTTGCTTTTGGCTTAGTGATAGTTTTATTTATTAGCGTTATTGCCGTTTTAATGAATTGGGAGTTGTCTAACAGTATCTTTAAAAACTATGAAAAGCTCAACTTATCCTTATCACTACTTAGTGGAATTATGGTTTCAGGTGGGATGATTCCAACCTTTTTCGTAATCTATTTCCGTGAATGA", "aa": "MHPDFKAVRFAFGLVIVLFISVIAVLMNWELSNSIFKNYEKLNLSLSLLSGIMVSGGMIPTFFVIYFRE", "aa_hexdigest": "7833b4eeb50329d2239de1359567d57b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7876.366299999994, "isoelectric_point": 8.16248722076416 }, "id": "CNOCLNGIAL_1339", "locus": "GCA000143945_01339" }, { "type": "cds", "sequence": "contig_1", "start": 758325, "stop": 758801, "strand": "+", "frame": 3, "gene": "cdsA", "product": "Putative phosphatidate cytidylyltransferase", "db_xrefs": [ "COG:COG0575", "COG:I", "EC:2.7.7.41", "GO:0004605", "GO:0005886", "GO:0016024", "KEGG:K00981", "SO:0001217", "UniRef:UniRef50_Q49433", "UniRef:UniRef90_P75160" ], "nt": "ATGATTGTCTTTTTATGGGCAGTGTACATGATTAGCACTTCCTTCATAAAGATCCGACCATCATTGCAAGTGATTTATAGCCTAGGTGCAGTGATCTGTTTCATCGCTTGCATAGGTACAATTTACTTTTCCGTAATTAGGGGATGGACTACCATTTTTCTCTTAATTGCTATTGGCGTTTGCACTGATACCTTTGCTTATCTGTTTGGTAAACGTTTTGGAAAAAATCCCCTCATTAAAATTTCTCCCTCAAAAACTTGGGAAGGTGCTTTCTTTGGGGTTACTGGTACAGTTTTAACCATTTCTATTATTTGTGTTTTGTACTCGATCCCTAACTATGTAAGACAACCTAGCATAAAGGATGCAAGTAAGACAGCACTACAAACACCACAAAACTATGATGTGCATAACCTTATTACCAATGTCTTTCTCATCTCCTTCATTTCCGGTGGTAGTACCTTTTACATCTACTGGTGA", "aa": "MIVFLWAVYMISTSFIKIRPSLQVIYSLGAVICFIACIGTIYFSVIRGWTTIFLLIAIGVCTDTFAYLFGKRFGKNPLIKISPSKTWEGAFFGVTGTVLTISIICVLYSIPNYVRQPSIKDASKTALQTPQNYDVHNLITNVFLISFISGGSTFYIYW", "aa_hexdigest": "c40ab368dd201c84e57d8d7d9d61fa57", "start_type": "ATG", "rbs_motif": null, "genes": [ "cdsA" ], "truncated": "both", "pseudogene": { "start": 758025, "stop": 759056, "observations": { "mutation": [ 758280, 758298, 758304, 758799 ], "stop": [ 758280, 758298, 758304, 758799 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75160", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 312.0, "evalue": 2.44e-104, "gene_start": 1, "gene_end": 158, "reference_start": 153, "reference_end": 310, "reference_sequence": "MDLKDNSFAKKRSTVFVVLLIVFCFFLMFSAFADGFNFWSPWSTDFNSRVIHIKSDGNIETTSIVSHELHPDFKAVRFAFGLVIVLFISVIAVLMNWELSNSIFKNYEKLNLSLSLLSGIMVSGGMIPTFFVIYFREWNATVNWIWTASFAGMIVFLWAVYMISTSFIKIRPSLQVIYSLGAVICFIACIGTIYFSVIRGWTTIFLLIAIGVCTDTFAYLFGKRFGKNPLIKISPSKTWEGAFFGVTGTVLTISIICVLYSIPNYVRQPSIKDASKTALQTPQNYDVHNLITNVFLISFISGGSTFYIYWWVSTLALIFTASIFAIGGDLFFSYIKRLTKIKDFSKLLGKHGGILDRFDSSSFLISFFFIYHVIAGISSNQRLLMEPNTYFSAVS" }, "paralog": false, "identity": 0.9854227405247813, "score": 660.0, "evalue": 1.26e-243, "description": "Internal stop codon at 758280, 758298, 758304, 758799. Nonsense mutation around 758280, 758298, 758304, 758799.", "psc": { "uniref90_id": "UniRef90_P75160", "gene": "cdsA", "product": "Putative phosphatidate cytidylyltransferase", "ec_ids": [ "2.7.7.41" ], "uniref50_id": "UniRef50_Q49433", "cog_id": "COG0575", "cog_category": "I", "kegg_orthology_id": "K00981", "go_ids": [ "GO:0005886", "GO:0004605", "GO:0016024" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75160", "EC:2.7.7.41", "UniRef:UniRef50_Q49433", "COG:COG0575", "COG:I", "KEGG:K00981", "GO:0005886", "GO:0004605", "GO:0016024" ] }, "pscc": { "uniref50_id": "UniRef50_Q49433", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49433" ], "product": "Putative phosphatidate cytidylyltransferase" } }, "id": "CNOCLNGIAL_1340", "locus": "GCA000143945_01340" }, { "type": "cds", "sequence": "contig_1", "start": 759160, "stop": 759651, "strand": "+", "frame": 1, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF638P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_P75159" ], "nt": "ATGCTCGAAAAAGAGCTATTGGCGCCCGATGGTAAATACGAATACTTTAACGGTGGTATTAAAGCCTCTGGACGCACCAATGAGTTCAATACCTTTAAGAACACCATTAGCATTATCATAGGCGGTTCTTGTGGTTATGTCAGATTAGCCGATAAGGATTACTTCTGTGGCCAGAGTAGCTGTACCCTAACTGTTTTAGATCCCCTGGAAATTGACCTCAAGTTTGCTTATTATGCACTTAAATCACAAGAGGAAAAAATTACCAGTTTAGCTAGCGGAACTACCATTAAAAACATTCGCCTAAGTGATTTAAAGGATTTACCTATTCCGCTTGTTAAAAGCATTCAGGATCAACGAACTATTGCACATGCTTTAAGTGTGTTTGACCTTCGCATCGAACACCTCAATGAATTAATTGAAGTAAACCGTAAGCTCAGAGACGAGTATGCCCACAAATTATTTACCTTAGATCCAGACTTCTTAACACACTGA", "aa": "MLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTH", "aa_hexdigest": "a1858b4425475498eaa5bee35fab524d", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 759076, "stop": 759954, "observations": { "mutation": [ 759112, 759649 ], "stop": [ 759112, 759649 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75159", "query_cov": 1.0, "subject_cov": 0.43466666666666665, "identity": 1.0, "score": 328.0, "evalue": 6.72e-111, "gene_start": 1, "gene_end": 163, "reference_start": 29, "reference_end": 191, "reference_sequence": "MTPKLKLNTNSNWTKKTLGSLFELKKGEMLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTHWNLHELHEQMGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNKTYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEECKEEAKSLISIRDNLLGKLFPTLS" }, "paralog": false, "identity": 0.9897260273972602, "score": 583.0, "evalue": 2.13e-213, "description": "Internal stop codon at 759112, 759649. Nonsense mutation around 759112, 759649.", "psc": { "uniref90_id": "UniRef90_P75159", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF638P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75159", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "CNOCLNGIAL_1341", "locus": "GCA000143945_01341" }, { "type": "cds", "sequence": "contig_1", "start": 759676, "stop": 760203, "strand": "+", "frame": 1, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF638P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_P75159" ], "nt": "ATGGGTGAAATTAGTTTAGGTGAAGTGTTTCACTTAAAGAGTGGTAAATACTTAAAGGCTGATGAGCGCTTTGAGGATGGCAAATTCCCTTATTATGGTGCTGGTATTGAAAGCACTAGCTTTGTCAATGAACCCAACACCAAAGGTGATACTCTTTCAATGATTGCTAACGGTTACTCCATCGGAAACATTCGCTACCATACTATTCCTTGGTTTAATGGTACTGGTGGTATTGCCATGGAAGCATTAAAACCTAATGAAACCTATGTGCCGTTCTTTTACTGTGCTCTAAAATACATGCAAAAGGATCTAAAGGAACGCTTTAAACGTGATGAATCACCGTTTATTTCCTTAAAACTAGCCGGTGAAATTAAGGTTCCTTTCGTTAAATCGTTTGCACTCCAAAGAAAAGCCGGTAAGATTATTTACTTGTTAGATAAAACTCTTGAAGAATACAAAGAAGAAGCTAAGTCTTTAATCAGTATTCGCGATAACTTATTGGGCAAATTGTTCCCAACACTATCTTAA", "aa": "MGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNETYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEEYKEEAKSLISIRDNLLGKLFPTLS", "aa_hexdigest": "9dccecccb9e9661e97321afb879f71b4", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "5-prime", "pseudogene": { "start": 759376, "stop": 760203, "observations": { "mutation": [ 759649 ], "stop": [ 759649 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75159", "query_cov": 1.0, "subject_cov": 0.4666666666666667, "identity": 0.9890000000000001, "score": 351.0, "evalue": 1.21e-119, "gene_start": 1, "gene_end": 175, "reference_start": 201, "reference_end": 375, "reference_sequence": "MTPKLKLNTNSNWTKKTLGSLFELKKGEMLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTHWNLHELHEQMGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNKTYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEECKEEAKSLISIRDNLLGKLFPTLS" }, "paralog": false, "identity": 0.9890909090909091, "score": 541.0, "evalue": 9.31e-197, "description": "Internal stop codon at 759649. Nonsense mutation around 759649.", "psc": { "uniref90_id": "UniRef90_P75159", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF638P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75159", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "CNOCLNGIAL_1342", "locus": "GCA000143945_01342" }, { "type": "cds", "sequence": "contig_1", "start": 760200, "stop": 760808, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_639", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75158", "UniRef:UniRef90_P75158" ], "nt": "TTGCTTTCACAGAGAATATTAGAACGTTGGCAAACTGAAGTAGGCCGCTTTGCTAAATCCTTCGGCAACGGTAGTAACCAAAACAGTGTTCAACCCAATGCTACGCTTAAGGGTTTAAAGTTAAGTGAACGTTCAACAACTGAGATTCAATTATTAGCTGAACAAGCCATCACTGTTAGCAAGCAAGAAGTGAAGGAGTTTACCTACAAAGTTGAATCGAATAAGCAATTTGAGGCTACGGTTAAGATTAAGGCAGATTTAAAAATTGATCCAACCAAGGCGATGAGCCATATAGAGAATATTTTTAAGGATGATGAAACTAAGAAAAAAGATGCTCAAAATTCTTTAACCATGAGTATGGGTCAAAATGAAGCTCTAGAAGCCACTTTTACATATAGTCCAGCTACTCAAGGCATCTTTGGCCGCGCTTCATTTGACCGTTTTACCAGTAACATCAAACTTAACACAAAGTTAAGAATTCAAGTTTCTAGTACGAGTGATCTAATGAAGAAGTTATTGGAGAATAGCTTAACCAGCAGTCTCAAAGACCAGAGCTTTGATAACGAAGGTGTTAACCTTTTCCCTTACACCTTATTTGCTTTGCTTTAA", "aa": "MLSQRILERWQTEVGRFAKSFGNGSNQNSVQPNATLKGLKLSERSTTEIQLLAEQAITVSKQEVKEFTYKVESNKQFEATVKIKADLKIDPTKAMSHIENIFKDDETKKKDAQNSLTMSMGQNEALEATFTYSPATQGIFGRASFDRFTSNIKLNTKLRIQVSSTSDLMKKLLENSLTSSLKDQSFDNEGVNLFPYTLFALL", "aa_hexdigest": "50c6448ff0fe4eb386c73cda3d2a38fd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 760200, "stop": 761063, "observations": { "mutation": [ 760820, 761024, 761030 ], "stop": [ 760820, 761024, 761030 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75158", "query_cov": 1.0, "subject_cov": 0.7038327526132404, "identity": 0.995, "score": 374.0, "evalue": 2.73e-129, "gene_start": 1, "gene_end": 202, "reference_start": 86, "reference_end": 287, "reference_sequence": "MLGSMALKLRKWIWASIPSLALILSSCSALVASVELKSLSELQNLADRNTELTKNKRTLITTLRESYGLNPKGTLNLLFEGWRYTLLSQRILERWQTEVGRFAKSFGNGSNQNSVQPNATLKGLKLSERSTTEIQLLAEQAITVSKQEVKEFTYKVESNKQFEATVKIKADLKIDPTKAMSHIENIFKDDETKKKDAQNSLTMSMGQNEALEATFTYSPATQGIFGRASFDRFTSNIKLNTKLRIQVSSTSDLMKKLLENSLTSSLKDQSFDNEGVNLFPYTLFALL" }, "paralog": false, "identity": 0.9895470383275261, "score": 536.0, "evalue": 7.1e-196, "description": "Internal stop codon at 760820, 761024, 761030. Nonsense mutation around 760820, 761024, 761030.", "psc": { "uniref90_id": "UniRef90_P75158", "product": "Uncharacterized protein MPN_639", "uniref50_id": "UniRef50_P75158", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75158", "UniRef:UniRef50_P75158" ] }, "pscc": { "uniref50_id": "UniRef50_P75158", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75158" ], "product": "Uncharacterized protein MPN_639" } }, "id": "CNOCLNGIAL_1343", "locus": "GCA000143945_01343" }, { "type": "cds", "sequence": "contig_1", "start": 761120, "stop": 761500, "strand": "-", "frame": 2, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75157" ], "nt": "ATGGATGGAAAGATTGATGAATCAAGCAAAGCATTGATTAAATCATTCTTAGAGGGTAATAGTTCGGGAGGTAAAGGTAGCAATGGTAAAAACAGTATTGATGAAAGTGAATACACTGGTGAAAAAGCCAAATTCACTGGTAACTTTATTTTTACCTATACCCCACCAACTGGAGGTGCTAGAAACTTCTCTGATAAATCCTTTGATGTACCAACTAGTTCGATTAACTTTCCCGCTAATGTCAAGATTGATGTAACAACTTCTCACACAAAGCTGAATGAATTATTAGAAAGCAACGAACAGGTCAAAAAGATGAAAAGTCGTCAATTAACAGGTAAGTTATTTAATTTATTACCGTTTTTTTACTACGCTCTGCTTTAA", "aa": "MDGKIDESSKALIKSFLEGNSSGGKGSNGKNSIDESEYTGEKAKFTGNFIFTYTPPTGGARNFSDKSFDVPTSSINFPANVKIDVTTSHTKLNELLESNEQVKKMKSRQLTGKLFNLLPFFYYALL", "aa_hexdigest": "1e19667fd9bf88131ba2f591a06c6b4e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 761071, "stop": 761800, "observations": { "insertion": [ 761143 ], "mutation": [ 761542, 761758 ], "stop": [ 761542, 761758 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75157", "query_cov": 0.9603174603174603, "subject_cov": 0.4033333333333333, "identity": 1.0, "score": 233.0, "evalue": 5.79e-75, "gene_start": 1, "gene_end": 121, "reference_start": 159, "reference_end": 279, "reference_sequence": "MKLKLLLIPLLGSSLLLSACSSATSQVISSLSSAQKYFEANKGELNKKNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLERNLDASRFASAFGSTSKKDDITPNISEKSLFLADTFPGISSEIAKVFKVEKQTVSGFSYSWNSPKKFQVNIQIKMDGKIDESSKALIKSFLEGNSSGGKGSNGKNSIDESEYTGEKAKFTGNFIFTYTPPTGGARNFSDKSFDVPTSSINFPANVKIDVTTSHTKLNELLESNEQVKKMKSRQLTGKLFNLLPFFTTLCFNSFSPFTVFAVIFTIV" }, "paralog": false, "identity": 0.9917355371900827, "score": 464.0, "evalue": 3.57e-168, "description": "Internal stop codon at 761542, 761758. Frameshift due to insertion around 761143. Nonsense mutation around 761542, 761758.", "psc": { "uniref90_id": "UniRef90_P75157", "product": "Uncharacterized lipoprotein MG439 homolog 5", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75157", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "CNOCLNGIAL_1344", "locus": "GCA000143945_01344" }, { "type": "cds", "sequence": "contig_1", "start": 761540, "stop": 761743, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGATCAAAAGCTTTTAGAAAGAAATTTAGATGCTTCACGCTTTGCTAGCGCTTTTGGTTCTACTAGCAAGAAGGACGATATTACCCCTAACATTAGTGAAAAAAGTTTATTTTTAGCAGACACTTTTCCTGGTATTAGTTCAGAAATCGCTAAAGTATTTAAAGTTGAAAAGCAAACAGTTAGTGGTTTTTCTTACAGTTGA", "aa": "MDQKLLERNLDASRFASAFGSTSKKDDITPNISEKSLFLADTFPGISSEIAKVFKVEKQTVSGFSYS", "aa_hexdigest": "34706520f715a4c0fd24b91346f9bf69", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 68, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 3.4999555545073998e-28, "score": 94.14722442626953, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 7380.2125, "isoelectric_point": 6.140964317321777 }, "id": "CNOCLNGIAL_1345", "locus": "GCA000143945_01345" }, { "type": "cds", "sequence": "contig_1", "start": 761756, "stop": 761974, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAAACTTAAACTGTTATTAATTCCTTTATTAGGATCTAGTTTATTGCTGAGCGCTTGTTCCTCTGCAACTAGCCAGGTTATATCGAGTTTAAGTAGCGCGCAAAAATACTTTGAAGCAAATAAAGGTGAATTGAACAAAAAGAACGTTATCAACATTCTGAAAGATGGCTATAACTCTGATCCTAACAAAACAGTTAACGCTCTTTTAGCAGGTTGA", "aa": "MKLKLLLIPLLGSSLLLSACSSATSQVISSLSSAQKYFEANKGELNKKNVINILKDGYNSDPNKTVNALLAG", "aa_hexdigest": "3be1533c2100bb96a1ce5416f96fd50b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.5277777777777778, "hmm_cov": 1.0, "evalue": 1.3946684989430264e-12, "score": 43.08498001098633, "start": 35, "stop": 72 } ], "seq_stats": { "molecular_weight": 7637.823099999997, "isoelectric_point": 9.46507091522217 }, "id": "CNOCLNGIAL_1346", "locus": "GCA000143945_01346" }, { "type": "cds", "sequence": "contig_1", "start": 761974, "stop": 762141, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG440-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniParc:UPI000310140D", "UniRef:UniRef100_P75156", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P75156" ], "nt": "ATGAAGAAAATTAATTTCAATACCACCTTAAAAATAGATATGGTTGGTAAAGACGAAATCATGAAAAAGATTCTTCAAAGTACTACCTTTACTAATAATTTGTCCAGTCAAACTTTCCAAGACCAATCAATTGATCTTTTACCTTACTTGCTTTATTCCATTCTCTAA", "aa": "MKKINFNTTLKIDMVGKDEIMKKILQSTTFTNNLSSQTFQDQSIDLLPYLLYSIL", "aa_hexdigest": "ed0e169656d16334e5d49495f57ca318", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000310140D", "uniref100_id": "UniRef100_P75156", "db_xrefs": [ "SO:0001217", "UniParc:UPI000310140D", "UniRef:UniRef100_P75156" ] }, "ips": { "uniref100_id": "UniRef100_P75156", "uniref90_id": "UniRef90_P75156" }, "psc": { "uniref90_id": "UniRef90_P75156", "product": "Uncharacterized lipoprotein MG440 homolog 3", "uniref50_id": "UniRef50_P47678", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" }, "genes": [], "id": "CNOCLNGIAL_1347", "locus": "GCA000143945_01347" }, { "type": "cds", "sequence": "contig_1", "start": 762141, "stop": 762803, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386F78", "UniRef:UniRef100_A0AB38W594", "UniRef:UniRef50_A0AB38W594", "UniRef:UniRef90_A0AB38W594" ], "nt": "ATGAATAAGAAAAGACTATTACCCAAAGCTTCATTAGGTGCTTTATTCATGTTGTTCGGAACAGCTTTAACTGCTTGTTCTAATTCTGATTTTCAAACTAATTTAACAAGTTTGAATCAATTGAGGGAAGGCGTTAATCAAAATACCAGTCTAACCCAAGACAAGAAAGCTTTTGTGGAGTCACTGCAAAAAGCATTTGAAAATAATCCTGAAGGAACTACCAAAGTTTTATTAGACGCTTGGAAATTTACCTTATTAGATTCCAAAATTTTGGAGAGCAAAGACCCTTCTAGATTTGTCAAAGCTTTTGGCTCTGGAAAAAGTAATGAAGATGTTGAGCCTAATGCTAGTGTTAAAGGTTTAAGACTTGATAAACGATTTGAACCAAGCACTGCTAATATTATCAACAATGTTATTTCGCTAAATGAGCAAAAAGTAGAAGCATTTAATATCCAATACAAAAGTCGAACTAGTTTTAAAGTTCAAGTAAAACTTAATGCTCAAGGAAAATACCAAAAAAGTCAAGTTCAATCTTATCTACAACAAATTGGTTTAAATGATGGTGATTTGAAACAAGAAAGTGGCACTCTTTCTGCAGATCTTATTTTTACCTATACTGTACCTGAATCAAATCTTTTCTCTAGAAAGAACTTCGTACTTTAA", "aa": "MNKKRLLPKASLGALFMLFGTALTACSNSDFQTNLTSLNQLREGVNQNTSLTQDKKAFVESLQKAFENNPEGTTKVLLDAWKFTLLDSKILESKDPSRFVKAFGSGKSNEDVEPNASVKGLRLDKRFEPSTANIINNVISLNEQKVEAFNIQYKSRTSFKVQVKLNAQGKYQKSQVQSYLQQIGLNDGDLKQESGTLSADLIFTYTVPESNLFSRKNFVL", "aa_hexdigest": "86861385c155b139ed644e74d99c8666", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386F78", "uniref100_id": "UniRef100_A0AB38W594", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386F78", "UniRef:UniRef100_A0AB38W594" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W594", "uniref90_id": "UniRef90_A0AB38W594" }, "psc": { "uniref90_id": "UniRef90_A0AB38W594", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AB38W594" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W594" ], "product": "Lipoprotein" }, "genes": [], "id": "CNOCLNGIAL_1348", "locus": "GCA000143945_01348" }, { "type": "cds", "sequence": "contig_1", "start": 762806, "stop": 763171, "strand": "-", "frame": 2, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75155" ], "nt": "ATGGAAGGGATGATTAATGAAAAGAGTAAGGAAACAATTAAGTCTTTTTTGAGTAACAAAGATTCAAATGGAAGTAATAGCGTAGAAGAAAGTGAATATACTGGAGATAAAGCTAAATTCACTGCAGACTTTATCTTTTCCTATACTCCACCAACTGGAGGTACTAGATCTTTAACCGATAAATCATTTGATGTTATCACCAATATGATCAATTTCCCTGCTGATGTAAAGATTGATGTGAACACTTCGCATAATAAACTAAATGAACTGCTTGAGAAAAACGATCAAGTTAAGCGAATGAAAAGTCGTACATTTAATGGAAGAAATATTGACTTGCTACCCTTCTTTTACTATGCTCTACTTTAA", "aa": "MEGMINEKSKETIKSFLSNKDSNGSNSVEESEYTGDKAKFTADFIFSYTPPTGGTRSLTDKSFDVITNMINFPADVKIDVNTSHNKLNELLEKNDQVKRMKSRTFNGRNIDLLPFFYYALL", "aa_hexdigest": "0f6a0c56917a4bae56a36bb06b24bb2e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 762806, "stop": 763471, "observations": { "mutation": [ 763213 ], "stop": [ 763213 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75155", "query_cov": 1.0, "subject_cov": 0.4336917562724014, "identity": 1.0, "score": 234.0, "evalue": 1.22e-75, "gene_start": 1, "gene_end": 121, "reference_start": 159, "reference_end": 279, "reference_sequence": "MKLKLYLIPLLASGIILSACSSTTSQVISSLSSAQKYFEANKGELNKNNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLENNLDPSRFKNTFGPNKNKDDVTPNISEKGLFLNETFTGLGSQIAEVFNVQKQIVSGFSYSWTSPKSFKVNIHIKMEGMINEKSKETIKSFLSNKDSNGSNSVEESEYTGDKAKFTADFIFSYTPPTGGTRSLTDKSFDVITNMINFPADVKIDVNTSHNKLNELLEKNDQVKRMKSRTFNGRNIDLLPFFYYALL" }, "paralog": false, "identity": 0.995475113122172, "score": 441.0, "evalue": 9.27e-160, "description": "Internal stop codon at 763213. Nonsense mutation around 763213.", "psc": { "uniref90_id": "UniRef90_P75155", "product": "Uncharacterized lipoprotein MG439 homolog 4", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75155", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "CNOCLNGIAL_1349", "locus": "GCA000143945_01349" }, { "type": "cds", "sequence": "contig_1", "start": 763211, "stop": 763645, "strand": "-", "frame": 2, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75155" ], "nt": "ATGAAACTTAAACTTTATTTAATTCCTCTTTTAGCTTCCGGCATTATTTTAAGTGCATGTTCTTCCACTACCAGCCAGGTTATATCGAGTTTAAGTAGCGCTCAAAAATACTTTGAAGCAAATAAAGGTGAGTTGAATAAAAATAACGTTATCAACATTCTGAAAGATGGCTATAACTCTGATCCTAACAAAACAGTTAACGCCTTACTAGCAGGCTGGAAGTACACCTTAATGGATCAAAAATTGTTGGAAAATAATTTAGATCCATCTCGTTTTAAAAATACTTTTGGCCCGAATAAAAATAAAGATGACGTTACTCCGAATATTAGCGAGAAAGGTTTATTTTTAAATGAAACTTTTACTGGTTTAGGAAGTCAAATTGCTGAAGTGTTTAATGTACAAAAACAAATTGTTAGTGGTTTTTCTTACAGCTGA", "aa": "MKLKLYLIPLLASGIILSACSSTTSQVISSLSSAQKYFEANKGELNKNNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLENNLDPSRFKNTFGPNKNKDDVTPNISEKGLFLNETFTGLGSQIAEVFNVQKQIVSGFSYS", "aa_hexdigest": "1d0e857d1833acb15c84bc7e24d1f425", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 762908, "stop": 763645, "observations": { "mutation": [ 763213 ], "stop": [ 763213 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75155", "query_cov": 1.0, "subject_cov": 0.5161290322580645, "identity": 1.0, "score": 275.0, "evalue": 1.23e-91, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MKLKLYLIPLLASGIILSACSSTTSQVISSLSSAQKYFEANKGELNKNNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLENNLDPSRFKNTFGPNKNKDDVTPNISEKGLFLNETFTGLGSQIAEVFNVQKQIVSGFSYSWTSPKSFKVNIHIKMEGMINEKSKETIKSFLSNKDSNGSNSVEESEYTGDKAKFTADFIFSYTPPTGGTRSLTDKSFDVITNMINFPADVKIDVNTSHNKLNELLEKNDQVKRMKSRTFNGRNIDLLPFFYYALL" }, "paralog": false, "identity": 0.9959183673469387, "score": 482.0, "evalue": 1.65e-175, "description": "Internal stop codon at 763213. Nonsense mutation around 763213.", "psc": { "uniref90_id": "UniRef90_P75155", "product": "Uncharacterized lipoprotein MG439 homolog 4", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75155", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "CNOCLNGIAL_1350", "locus": "GCA000143945_01350" }, { "type": "cds", "sequence": "contig_1", "start": 763645, "stop": 764103, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_643", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P75154" ], "nt": "GTGGAAAGATACACACAAGACACTGCCAATATAATTAATAACGTAATTAAACTGGACAAACAAAAAGTAGAAGCTTTTAGCATTCAATACAAAGATCCTAAAAACTTTAGAGTTCAAGTAAAAATTAATGCTAAAGGTAATTACAAAAAGGATACTGTAAAAACTTATTTAAGTCAAGTAGGTTTATCAGATGGTGATCTAAATGATACAGGTACATTAGAAGCTGAAATAATTTACACCTATATGCCACCTGCTGCCAGTTTCTTTTCTGCAAGTAAATTTGACAAACTAACAAGAGCCATCAACTTTAATACCAATTTACGAATACAAATTATAGGCAAAGATAGTGTGATGACTAAATTATTGCAACAAAGTAGTTTTGTAAAGCAACTAGCTGATCAAAAATTTCAAGACCAATCGATAAATCTCTTGCCTTACGTGCTTTACTCCATACTCTAA", "aa": "MERYTQDTANIINNVIKLDKQKVEAFSIQYKDPKNFRVQVKINAKGNYKKDTVKTYLSQVGLSDGDLNDTGTLEAEIIYTYMPPAASFFSASKFDKLTRAINFNTNLRIQIIGKDSVMTKLLQQSSFVKQLADQKFQDQSINLLPYVLYSIL", "aa_hexdigest": "9c3439f16ee6c689de554c12e96f4e75", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 763645, "stop": 764403, "observations": { "mutation": [ 764232 ], "stop": [ 764232 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75154", "query_cov": 1.0, "subject_cov": 0.5033112582781457, "identity": 0.993, "score": 290.0, "evalue": 4.3e-97, "gene_start": 1, "gene_end": 152, "reference_start": 151, "reference_end": 302, "reference_sequence": "MLKKLKVVRLLVNHLIYCPSIFMPYSKNMKKKIWNKTSLGALFMLFGTALTACSNSGFEANLTSLNQLRTSASKNTNLTQNKADLVTALKSAFENNPEGTTRVLLDAWKFTLLDAQILEKQDFSKFSKSFGSGRSIEDVEPSAGVRGLRLVERYTQDTANIINNVIKLDKQKVEAFSIQYKDPKNFRVQVKINAKGNYKKDTVKTYLSQVGLSDGDLNDTGTLEAEIIYTYMPPAASFFSASKFDKLTRAINFNTNLRIQIIGKDSVMTKLLQQSSFVKQLADQKFQDQSINLLPYVLYSIL" }, "paralog": false, "identity": 0.996031746031746, "score": 484.0, "evalue": 9.01e-176, "description": "Internal stop codon at 764232. Nonsense mutation around 764232.", "psc": { "uniref90_id": "UniRef90_P75154", "product": "Uncharacterized protein MPN_643", "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75154", "UniRef:UniRef50_P47678" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" } }, "id": "CNOCLNGIAL_1351", "locus": "GCA000143945_01351" }, { "type": "cds", "sequence": "contig_1", "start": 764230, "stop": 764424, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGTTGTTTGGAACAGCTTTAACTGCTTGTTCTAATTCTGGTTTTGAAGCAAATCTTACTAGCTTGAATCAATTAAGAACCAGTGCAAGTAAAAATACTAATTTAACCCAAAATAAAGCTGATTTAGTTACAGCCTTAAAATCAGCTTTTGAAAATAATCCAGAAGGCACTACTAGGGTTTTATTAGACGCTTGA", "aa": "MLFGTALTACSNSGFEANLTSLNQLRTSASKNTNLTQNKADLVTALKSAFENNPEGTTRVLLDA", "aa_hexdigest": "1f85c5aa94dd5ce0d870cf36554211e7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.609375, "hmm_cov": 1.0, "evalue": 6.288796976303489e-11, "score": 37.6708984375, "start": 26, "stop": 64 } ], "seq_stats": { "molecular_weight": 6776.489100000002, "isoelectric_point": 6.036323738098144 }, "id": "CNOCLNGIAL_1352", "locus": "GCA000143945_01352" }, { "type": "cds", "sequence": "contig_1", "start": 764474, "stop": 764836, "strand": "-", "frame": 2, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75153" ], "nt": "ATGATGCAAGGCATTGTTGGCAGTGGTGAAGAAAGTAATTCTTTAATTAAATCTTTCTTGAGCTCTGGAAATAATGGCAATGTTACTGAAAATGATTTTAATAATGGAAATGCAAACTTTGATGGCACATTCATATTCACTTTTACTCCGCCTACAGATGGAAGAAGATTTGCATTTAGTAACTTTGATCCAATCACTGGAACAATTAATTTCCCTGCTAATCTTCAAATTGATGCAAGCACAACTCATGAAAAACTCAATATCCTAATGCAAAACAACGAACATGTTAAAAAAATTAAAAGTCGTTCGTTTACTGGTAAATCATTTGATCTATTGCCCTTCTATTTTTATGCCTTACTCTAA", "aa": "MMQGIVGSGEESNSLIKSFLSSGNNGNVTENDFNNGNANFDGTFIFTFTPPTDGRRFAFSNFDPITGTINFPANLQIDASTTHEKLNILMQNNEHVKKIKSRSFTGKSFDLLPFYFYALL", "aa_hexdigest": "fe00e36e5c8379a0bf758de99fde893b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 764474, "stop": 765136, "observations": { "mutation": [ 764875, 765091 ], "stop": [ 764875, 765091 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75153", "query_cov": 1.0, "subject_cov": 0.42402826855123676, "identity": 1.0, "score": 236.0, "evalue": 1.15e-76, "gene_start": 1, "gene_end": 120, "reference_start": 164, "reference_end": 283, "reference_sequence": "MNKKRLLFRTPLDALFLLFGTALSACSSTATNVISSLSSAQKYFDAHKSELIKKNVINLLKEGYSTDSKATVNSLFAGWKYTLMDQKILERNLDASRFTKAFGTNKGKDDVIPSISEKGLFLDETYSGVSQQIAKVLGVQSQKVTGFSYSWSSTTNFKVVISFMMQGIVGSGEESNSLIKSFLSSGNNGNVTENDFNNGNANFDGTFIFTFTPPTDGRRFAFSNFDPITGTINFPANLQIDASTTHEKLNILMQNNEHVKKIKSRSFTGKSFDLLPFYFYALL" }, "paralog": false, "identity": 0.9863636363636363, "score": 429.0, "evalue": 1.08e-154, "description": "Internal stop codon at 764875, 765091. Nonsense mutation around 764875, 765091.", "psc": { "uniref90_id": "UniRef90_P75153", "product": "Uncharacterized lipoprotein MG439 homolog 3", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75153", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "CNOCLNGIAL_1353", "locus": "GCA000143945_01353" }, { "type": "cds", "sequence": "contig_1", "start": 764873, "stop": 765076, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGATCAAAAAATACTGGAAAGAAATCTAGATGCTAGTCGCTTTACAAAAGCATTTGGTACTAACAAAGGCAAAGATGATGTCATTCCAAGTATTAGTGAGAAAGGTTTATTTTTAGATGAAACTTACAGTGGTGTAAGTCAACAAATAGTTAAAGTACTTGGAGTGCAAAGCCAAAAAGTAACTGGATTTTCTTACAGCTGA", "aa": "MDQKILERNLDASRFTKAFGTNKGKDDVIPSISEKGLFLDETYSGVSQQIVKVLGVQSQKVTGFSYS", "aa_hexdigest": "45669d012af78d63180a892ddc02fdca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 68, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 9.723833299735183e-30, "score": 99.24095916748047, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 7401.2778000000035, "isoelectric_point": 8.007634162902832 }, "id": "CNOCLNGIAL_1354", "locus": "GCA000143945_01354" }, { "type": "cds", "sequence": "contig_1", "start": 765089, "stop": 765325, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAATAAGAAAAGGCTATTATTCAGAACTCCATTAGATGCTTTATTTTTATTGTTCGGCACTGCTTTAAGTGCCTGTTCATCAACTGCCACCAATGTTATTTCTAGTTTAAGTAGTGCGCAAAAATACTTTGATGCTCACAAAAGTGAACTAATCAAAAAGAATGTAATTAATCTTTTAAAAGAAGGTTACAGTACTGATTCAAAAGCTACAGTTAATTCACTTTTTGCTGGTTGA", "aa": "MNKKRLLFRTPLDALFLLFGTALSACSSTATNVISSLSSAQKYFDAHKSELIKKNVINLLKEGYSTDSKATVNSLFAG", "aa_hexdigest": "a27f1dd6ef62585b245e844e16b32470", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.48717948717948717, "hmm_cov": 1.0, "evalue": 5.719106816527627e-10, "score": 34.532745361328125, "start": 41, "stop": 78 } ], "seq_stats": { "molecular_weight": 8487.714099999994, "isoelectric_point": 9.666728019714355 }, "id": "CNOCLNGIAL_1355", "locus": "GCA000143945_01355" }, { "type": "cds", "sequence": "contig_1", "start": 765328, "stop": 765702, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75152" ], "nt": "ATGAAGGGAAAAGTTGGAAGTGATTCAGAGAGTCAACAATTAATTAAATCTTTTTTAAGTTCTGATAACAATGGATCAAATCAAAATGGCGGAGTAAAAGAAACTGACTTTAATGGTGATTCTGCTAATTTCGATGGTTTCTTTACTTTTACTTATACACCTCCTACTCAAAGTAGAAAATTTGGTGCCACTTCTTTTGACCCTCTTACAACTAAGATTAACTTTCCAGCAGACTTACAAATAGATGTAAGCACTACTCACCAAAAGTTAAATACTTTAATGGAAGCCAATGAACAAGTAAAACAGATTAAAAGCCGTAAGTTTACTGGTAAAACCTTTGACTTGTTACCGTTCTTTTACTACGCACTACTTTAA", "aa": "MKGKVGSDSESQQLIKSFLSSDNNGSNQNGGVKETDFNGDSANFDGFFTFTYTPPTQSRKFGATSFDPLTTKINFPADLQIDVSTTHQKLNTLMEANEQVKQIKSRKFTGKTFDLLPFFYYALL", "aa_hexdigest": "418b1dd1c030be37cf8acbe8ca0300f6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 765328, "stop": 766002, "observations": { "mutation": [ 765744, 765957 ], "stop": [ 765744, 765957 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75152", "query_cov": 1.0, "subject_cov": 0.4381625441696113, "identity": 1.0, "score": 241.0, "evalue": 2.91e-78, "gene_start": 1, "gene_end": 124, "reference_start": 160, "reference_end": 283, "reference_sequence": "MKLKLKFLLISLLGSSLLLSACSSAATQVISSLSSAQKYFESSQGELNKKNVIKILKEGYESDANKAVHALLAGWKYTLMDQQLLSKEVDSRFIKAFGSGRDKGDVTPSVSEKGLYLNETYTGFSSQIAKVLGVQSQTVKQFNYKWSSNSDFKVQIQISMKGKVGSDSESQQLIKSFLSSDNNGSNQNGGVKETDFNGDSANFDGFFTFTYTPPTQSRKFGATSFDPLTTKINFPADLQIDVSTTHQKLNTLMEANEQVKQIKSRKFTGKTFDLLPFFYYALL" }, "paralog": false, "identity": 0.9866071428571429, "score": 435.0, "evalue": 4.64e-157, "description": "Internal stop codon at 765744, 765957. Nonsense mutation around 765744, 765957.", "psc": { "uniref90_id": "UniRef90_P75152", "product": "Uncharacterized lipoprotein MG439 homolog 2", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75152", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "CNOCLNGIAL_1356", "locus": "GCA000143945_01356" }, { "type": "cds", "sequence": "contig_1", "start": 765742, "stop": 765942, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGACCAGCAATTACTTTCTAAAGAAGTTGATAGTCGCTTTATCAAAGCTTTTGGTAGCGGACGCGATAAAGGCGATGTAACTCCTAGTGTTAGTGAAAAGGGATTGTACTTAAACGAAACTTACACTGGCTTTAGTATTCAAATTGCCAAAGTCTTAGGTGTACAAAGTCAAACTGTTAAACAATTTAATTACAAGTGA", "aa": "MDQQLLSKEVDSRFIKAFGSGRDKGDVTPSVSEKGLYLNETYTGFSIQIAKVLGVQSQTVKQFNYK", "aa_hexdigest": "9f13110410c906bd6cc59d00df36a033", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 68, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 4.231205656339622e-27, "score": 90.60437774658203, "start": 1, "stop": 66 } ], "seq_stats": { "molecular_weight": 7377.257900000001, "isoelectric_point": 9.04899196624756 }, "id": "CNOCLNGIAL_1357", "locus": "GCA000143945_01357" }, { "type": "cds", "sequence": "contig_1", "start": 765955, "stop": 766179, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAAACTGAAACTTAAATTTCTATTAATTCCTCTTTTAGGTTCTAGTTTGTTGTTAAGCGCTTGTTCTTCAGCAGCTACTCAAGTAATTTCTAGTTTAAGTAGTGCTCAAAAATACTTTGAATCCAGTCAAGGTGAACTCAACAAAAAGAATGTCATCAAAATCTTAAAGGAAGGTTATGAATCTGATGCTAATAAGGCAGTCCATGCTTTGCTAGCTGGTTGA", "aa": "MKLKLKFLLIPLLGSSLLLSACSSAATQVISSLSSAQKYFESSQGELNKKNVIKILKEGYESDANKAVHALLAG", "aa_hexdigest": "1c78d29c554ad9396126998d01590024", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.5135135135135135, "hmm_cov": 1.0, "evalue": 2.5581330000722013e-10, "score": 35.676395416259766, "start": 37, "stop": 74 } ], "seq_stats": { "molecular_weight": 7896.181899999997, "isoelectric_point": 9.41762218475342 }, "id": "CNOCLNGIAL_1358", "locus": "GCA000143945_01358" }, { "type": "cds", "sequence": "contig_1", "start": 766179, "stop": 766742, "strand": "-", "frame": 1, "gene": null, "product": "putative lipo MG440-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P75151" ], "nt": "TTGGAAAAACAAGATCCAGTTAAATTTGTTAAAGCATTTGGTAGCGGTAAAAGTAAGGAAGACATAGAACCTAGTGCTGGCGTACGTGGTTTAAGATTTGTTGAACGTTTTGGTGATAACACAGCTAGTCTTATTAAAAATGCAATTTTATTGGATCAACAAAAAGTAGAGGTTTTTAATATTCGCTTTAAAAGTAGTCGTGATTTCACTATTCAGATTAAGCTGAATGCAAAAGGTAATTACAAAAAGAGTGAAGTCGAGAAATATAAGTCACAAATTGGTCTTCAAGATAATGAACTGGGCGATAAGGAAGAAGGCACTTTAGAAGCTGATTTAATCTTCACTTACACTCCACCTGCTTCAAATTTGTTTTCTAAGAGTAATTTTGACAAGTTAACTAAATCGATTAATTTCAATACCAATCTGAAATTAGAAATGGTTGGTAAAGACGAAGTAATGAGAAAGATTCTTAACAGTAGTGCCTTCACTAATAATCTTGCCAGTGTTAATTACCCAAACCAAGCAGTTAATTTACTGCCTTACGTACTTTACTCCATCCTCTAA", "aa": "MEKQDPVKFVKAFGSGKSKEDIEPSAGVRGLRFVERFGDNTASLIKNAILLDQQKVEVFNIRFKSSRDFTIQIKLNAKGNYKKSEVEKYKSQIGLQDNELGDKEEGTLEADLIFTYTPPASNLFSKSNFDKLTKSINFNTNLKLEMVGKDEVMRKILNSSAFTNNLASVNYPNQAVNLLPYVLYSIL", "aa_hexdigest": "e027f7d16dff0dcbebeacd5c60a4ebf1", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 766179, "stop": 767012, "observations": { "mutation": [ 766772, 766994 ], "stop": [ 766772, 766994 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75151", "query_cov": 1.0, "subject_cov": 0.6750902527075813, "identity": 0.9890000000000001, "score": 356.0, "evalue": 1.06e-122, "gene_start": 1, "gene_end": 187, "reference_start": 91, "reference_end": 277, "reference_sequence": "MNKKSIWSKTAFGSLFLLLGTAFTACSNYDFQANLTSLNQLRTSANKDINLTQDKNTLIDALKTAFENNSEGTTKVLLDAWKFSLYDAKILEKQDPVKFVKAFGSGKSKEDIEPSAGVRGLRFVERFGDNTASLIKNAILLDQQKVEVFNIRFKSSRDFTIQIKLNAKGNYKKSEVEKYKSQIGVQDNELGDKEEGTLEADLIFTYTPPASNLFSKSNFDKLTKSINFNTNLKLEMVGKDEVMRKILNSSAFTNNLASVNYPNQAVNLLPYVLYSIL" }, "paralog": false, "identity": 0.9891696750902527, "score": 530.0, "evalue": 1.07e-193, "description": "Internal stop codon at 766772, 766994. Nonsense mutation around 766772, 766994.", "psc": { "uniref90_id": "UniRef90_P75151", "product": "Uncharacterized lipoprotein MG440 homolog 1", "uniref50_id": "UniRef50_P47678", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75151", "UniRef:UniRef50_P47678", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" } }, "id": "CNOCLNGIAL_1359", "locus": "GCA000143945_01359" }, { "type": "cds", "sequence": "contig_1", "start": 766770, "stop": 766970, "strand": "-", "frame": 1, "gene": null, "product": "lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912A3C4", "UniRef:UniRef90_UPI001912A3C4" ], "nt": "TTGTTTCTTTTATTAGGAACAGCGTTTACTGCTTGTTCTAACTATGACTTTCAAGCTAACTTAACTAGTTTGAACCAATTGAGAACTTCAGCCAATAAAGACATTAATTTAACTCAGGACAAAAATACCTTAATTGATGCTTTAAAAACTGCCTTTGAAAATAATTCAGAAGGTACCACTAAGGTTTTACTAGATGCCTGA", "aa": "MFLLLGTAFTACSNYDFQANLTSLNQLRTSANKDINLTQDKNTLIDALKTAFENNSEGTTKVLLDA", "aa_hexdigest": "c535947d0bfa283cab62c1a492dee459", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 766692, "stop": 767012, "observations": { "mutation": [ 766772, 766994 ], "stop": [ 766772, 766994 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001912A3C4", "query_cov": 1.0, "subject_cov": 0.6226415094339622, "identity": 0.985, "score": 124.0, "evalue": 1.27e-35, "gene_start": 1, "gene_end": 66, "reference_start": 15, "reference_end": 80, "reference_sequence": "MNKKSIWSKTAFGSLFLLLGTAFTACSNYDFQANLTSLNQLRTSANKDINLTQDKNTLIDALKTAFENNSEGTTKVLLDAWKFSLYDAKILEKQDRVKFVKAFGRG" }, "paralog": false, "identity": 0.9622641509433962, "score": 196.0, "evalue": 2.89e-66, "description": "Internal stop codon at 766772, 766994. Nonsense mutation around 766772, 766994.", "psc": { "uniref90_id": "UniRef90_UPI001912A3C4", "product": "lipoprotein", "uniref50_id": "UniRef50_UPI001912A3C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001912A3C4", "UniRef:UniRef50_UPI001912A3C4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001912A3C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912A3C4" ], "product": "lipoprotein" } }, "id": "CNOCLNGIAL_1360", "locus": "GCA000143945_01360" }, { "type": "cds", "sequence": "contig_1", "start": 767035, "stop": 767418, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75150" ], "nt": "ATGAAAGGAAAAGTTGGTAGTGATGGCACAAGTCAAACTTTAATTAAGTCTTTTTTAGGTACTGATAGCAAAGGCAGTGGAAGCAATAATCAAAATGGTGGTGTAACTGAAAAAGATTTTGAAGGTGACCAAGCTAACTTTGATGGCAATTTTATCTTTACTTACACTCAACCTTCCGATGGCAGAAGACTTGCGTCCAGCAACTTTGATCCAATTACTGGAACCATCAATTTTCCTGCTGATCTCCAAATTGAGGTAAGTACTAGTCATGAAAAGTTAAACACTTTAATGACAACTAATACTCAGGTAGGAATGATTAAGAACCGCTCTTTTAAAGGCAAATCATTTAATCTTTTGCCTTTTTTTTACTACGCCCTTCTCTAA", "aa": "MKGKVGSDGTSQTLIKSFLGTDSKGSGSNNQNGGVTEKDFEGDQANFDGNFIFTYTQPSDGRRLASSNFDPITGTINFPADLQIEVSTSHEKLNTLMTTNTQVGMIKNRSFKGKSFNLLPFFYYALL", "aa_hexdigest": "4318a218e250f7d97a8026f9bc23c215", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 767028, "stop": 767718, "observations": { "insertion": [ 767058 ], "mutation": [ 767460, 767673 ], "stop": [ 767460, 767673 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75150", "query_cov": 0.9606299212598425, "subject_cov": 0.4178082191780822, "identity": 1.0, "score": 239.0, "evalue": 1.76e-77, "gene_start": 1, "gene_end": 122, "reference_start": 164, "reference_end": 285, "reference_sequence": "MNKKSILSKTSLGSLFFLFGTALSACSSATTEVISSFSSAQKYFSANKKELNKRNLVTILKDSYNSDPKSTVNSLLAGWKYSLLDQKLLENPMDPSRFSKAFGSNTKDDVTPNISEKGLYLAETYPGVSSQIAQVLGVQSQKVTGFSYSWTSKTKFEVKILIKMKGKVGSDGTSQTLIKSFLGTDSKGSGSNNQNGGVTEKDFEGDQANFDGNFIFTYTQPSDGRRLASSNFDPITGTINFPADLQIEVSTSHEKLNTLMTTNTQVGMIKNRSFKGKSFNLLPFFTTPFSNF" }, "paralog": false, "identity": 0.9912663755458515, "score": 443.0, "evalue": 3.23e-160, "description": "Internal stop codon at 767460, 767673. Frameshift due to insertion around 767058. Nonsense mutation around 767460, 767673.", "psc": { "uniref90_id": "UniRef90_P75150", "product": "Uncharacterized lipoprotein MG439 homolog 1", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75150", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "CNOCLNGIAL_1361", "locus": "GCA000143945_01361" }, { "type": "cds", "sequence": "contig_1", "start": 767458, "stop": 767661, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "TTGCTGGATCAAAAGTTATTGGAGAACCCAATGGATCCTTCCCGTTTTTCTAAAGCTTTTGGTTCTAACACTAAAGATGATGTTACCCCGAACATCAGTGAAAAAGGTTTATATCTTGCAGAAACTTATCCCGGTGTAAGTAGTCAAATCGCTCAAGTTCTTGGTGTCCAAAGCCAAAAGGTAACTGGTTTTTCTTATAGCTGA", "aa": "MLDQKLLENPMDPSRFSKAFGSNTKDDVTPNISEKGLYLAETYPGVSSQIAQVLGVQSQKVTGFSYS", "aa_hexdigest": "5a87f02c6a0b1acb82b95c677be11f7c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 69, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.6007324842794336e-29, "score": 98.53238677978516, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 7315.119000000002, "isoelectric_point": 5.042607688903809 }, "id": "CNOCLNGIAL_1362", "locus": "GCA000143945_01362" }, { "type": "cds", "sequence": "contig_1", "start": 767671, "stop": 767907, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAACAAAAAATCGATTTTGTCAAAGACCAGTCTAGGTTCTTTGTTTTTCCTGTTTGGCACAGCTTTAAGTGCCTGCTCCTCTGCCACCACAGAAGTAATTTCCAGTTTTAGTAGTGCCCAGAAGTACTTTAGTGCCAACAAAAAAGAACTTAATAAACGTAATCTAGTAACCATTTTGAAGGATAGTTATAATTCTGATCCCAAGTCAACAGTTAATTCTTTATTAGCTGGCTGA", "aa": "MNKKSILSKTSLGSLFFLFGTALSACSSATTEVISSFSSAQKYFSANKKELNKRNLVTILKDSYNSDPKSTVNSLLAG", "aa_hexdigest": "40b50d430d2f5d5994d1915b01417aac", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.48717948717948717, "hmm_cov": 1.0, "evalue": 5.8551829100801055e-12, "score": 41.045589447021484, "start": 41, "stop": 78 } ], "seq_stats": { "molecular_weight": 8388.492099999996, "isoelectric_point": 9.78032131195068 }, "id": "CNOCLNGIAL_1363", "locus": "GCA000143945_01363" }, { "type": "cds", "sequence": "contig_1", "start": 768264, "stop": 768428, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGCCAATTTAAAGCAAATGTACTAGCTATCTTACTAGTCTCCTTGTTTTTAATTAACGGTCTTGTTTTTCTTTCGAAAACATTATTTAAGTTATTTAATTTTCGTGTCACCCACTCATTAAATAACTACTATACCTTTAATCTGTTAATTAAAGCTTGA", "aa": "MKSQFKANVLAILLVSLFLINGLVFLSKTLFKLFNFRVTHSLNNYYTFNLLIKA", "aa_hexdigest": "7b062f5e42e9a4aca979e4c01b7d1285", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6268.4995, "isoelectric_point": 10.390772438049318 }, "id": "CNOCLNGIAL_1364", "locus": "GCA000143945_01364" }, { "type": "cds", "sequence": "contig_1", "start": 769315, "stop": 769725, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_649", "db_xrefs": [ "RefSeq:WP_026088303.1", "SO:0001217", "UniParc:UPI0002FDADE2", "UniRef:UniRef100_A0AB38W553", "UniRef:UniRef50_P75148", "UniRef:UniRef90_P75148" ], "nt": "TTGAAAATTTCCTTAAGCACTAGTTTCTTTAGTATCGAACAAAAGGTTGAGTATTTCAATTTGAATTACCAAAGCCTTAGTGATTTTTCAGTTGTTGCCAAATTGAACTATACCTTTACATGGTATGGCAATGATTTTTCAATAGGTTTCGCTCCAAAAAAAGGTGAAAAGCTTTATTTTGATCTCTTTTTTACTTTTAAAGCTTCTCCCAACCATCCATTTGCAGCAGAAAACTTTAAACCTGATTCAGAAGCAATCGACTTTTATGTTTCATTTAGCTGGAGATTAGAAGGAAAAGACGAAGTTAGCAAAAAACTTTTTGAATTAAGTGTTTTTGGTAGAGCAAGAGCTTTTCAAATTGATGACTACAAAATCAACCTTTTTAGTTATTTAGTTTACGTAATTAGATAA", "aa": "MKISLSTSFFSIEQKVEYFNLNYQSLSDFSVVAKLNYTFTWYGNDFSIGFAPKKGEKLYFDLFFTFKASPNHPFAAENFKPDSEAIDFYVSFSWRLEGKDEVSKKLFELSVFGRARAFQIDDYKINLFSYLVYVIR", "aa_hexdigest": "3f8795f955778ce042f31bc0b49cf18d", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002FDADE2", "ncbi_nrp_id": "WP_026088303.1", "uniref100_id": "UniRef100_A0AB38W553", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FDADE2", "RefSeq:WP_026088303.1", "UniRef:UniRef100_A0AB38W553" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W553", "uniref90_id": "UniRef90_P75148" }, "psc": { "uniref90_id": "UniRef90_P75148", "product": "Uncharacterized protein MPN_649", "uniref50_id": "UniRef50_P75148" }, "pscc": { "uniref50_id": "UniRef50_P75148", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75148" ], "product": "Uncharacterized protein MPN_649" }, "genes": [], "id": "CNOCLNGIAL_1365", "locus": "GCA000143945_01365" }, { "type": "cds", "sequence": "contig_1", "start": 769731, "stop": 770015, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_650", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75147", "UniRef:UniRef90_P75147" ], "nt": "ATGAAATTTAAATATCTATCAACTCCGTTATTATTTTCAGCTTTATTATTTAGCGCTTGTTCTAGCGTCAATGTTGGAGCTAATTTGAAAAGTCTTATTAAGGAAACCACCGATCAAGACCTTGATTTGTCTGAATCAGTTAGTACTTCTGAGGGAAAGAAGAATTTAATAAGTTCTTTAAAGAAGTCTTACGAAACTAACCCTAGCAAAACAGCTAGTGTACTCTTGAATGCCTGGAAGCAAAGTGTAGAAAACGGTCAAATAGAGAGTTCTGAAACTAATTGA", "aa": "MKFKYLSTPLLFSALLFSACSSVNVGANLKSLIKETTDQDLDLSESVSTSEGKKNLISSLKKSYETNPSKTASVLLNAWKQSVENGQIESSETN", "aa_hexdigest": "856656c27a4a32982dfc34ad2685c8b5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75147", "query_cov": 1.0, "subject_cov": 0.9306930693069307, "identity": 1.0, "score": 167.0, "evalue": 2.92e-52, "valid": true, "product": "Uncharacterized lipoprotein MPN_650", "uniref50_id": "UniRef50_P75147", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75147", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75147" ], "product": "Uncharacterized lipoprotein MPN_650" }, "genes": [], "id": "CNOCLNGIAL_1366", "locus": "GCA000143945_01366" }, { "type": "cds", "sequence": "contig_1", "start": 770015, "stop": 770152, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036F3933", "UniRef:UniRef100_A0AB38W7G3", "UniRef:UniRef50_A0AB38W7G3", "UniRef:UniRef90_A0AB38W7G3" ], "nt": "ATGAGACTAGAAGTTTTTCCTAAGGATGAAATAACCAAAAAACTTTTAGATAATGGTTCTATTGGAAAATTGGTAGATAAAATTTACTTTGATTACAAAGTAAATTTACTGCTATATCTGGTTTATTCAGTCCTCTAA", "aa": "MRLEVFPKDEITKKLLDNGSIGKLVDKIYFDYKVNLLLYLVYSVL", "aa_hexdigest": "b33f894509a27877cd9987bb4301d6d1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00036F3933", "uniref100_id": "UniRef100_A0AB38W7G3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036F3933", "UniRef:UniRef100_A0AB38W7G3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7G3", "uniref90_id": "UniRef90_A0AB38W7G3" }, "psc": { "uniref90_id": "UniRef90_A0AB38W7G3", "uniref50_id": "UniRef50_A0AB38W7G3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7G3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7G3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5308.276500000001, "isoelectric_point": 7.982878303527833 }, "id": "CNOCLNGIAL_1367", "locus": "GCA000143945_01367" }, { "type": "cds", "sequence": "contig_1", "start": 770177, "stop": 770410, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5Y3", "UniRef:UniRef90_A0AB38W5Y3" ], "nt": "TTGTTTGACATTCAGGCAAACAATAAAAATGATTTTAAGGTTGTTGTTAAAATGAACACAACTTTTAATGCTGATATTAATCAAAAACCAAATCAAGGAGGGTTAATAATGTCGGGCTTTGGCAACTACTTGCTTAATTTTGATCTTATTTACACATACAAACCATTTAAAAACAAACCAATTCTAGAGACAAGTATTGTTAAAAACTCTGATAATAAATTAGGGAATTTGTGA", "aa": "MFDIQANNKNDFKVVVKMNTTFNADINQKPNQGGLIMSGFGNYLLNFDLIYTYKPFKNKPILETSIVKNSDNKLGNL", "aa_hexdigest": "639af4a3159d0034284dc2ca5fc6b675", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W5Y3", "query_cov": 1.0, "subject_cov": 0.927710843373494, "identity": 1.0, "score": 156.0, "evalue": 2.21e-48, "valid": true, "uniref50_id": "UniRef50_A0AB38W5Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8761.966999999995, "isoelectric_point": 9.302352714538571 }, "id": "CNOCLNGIAL_1368", "locus": "GCA000143945_01368" }, { "type": "cds", "sequence": "contig_1", "start": 770931, "stop": 771083, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E56736E", "UniRef:UniRef90_UPI001E56736E" ], "nt": "GTGATTGCTGTCATTATTGCCATTGCTGTAATAGCTGCTGGTCAAACCGAAGCATTTACAAAAATTGTTGGTCAAAAATCAGTAATGTTTTTATGTGTGATGATATTCGTCCCAATTGCAGCGCTAATCTTAAAACACACTGAAAAGTTTTGA", "aa": "MIAVIIAIAVIAAGQTEAFTKIVGQKSVMFLCVMIFVPIAALILKHTEKF", "aa_hexdigest": "1524574f7646eb52f194d46a2ff77716", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 770931, "stop": 771143, "observations": { "mutation": [ 771081 ], "stop": [ 771081 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E56736E", "query_cov": 1.0, "subject_cov": 0.7142857142857143, "identity": 0.96, "score": 84.0, "evalue": 2.39e-20, "gene_start": 1, "gene_end": 50, "reference_start": 1, "reference_end": 50, "reference_sequence": "MIAVIIAIAVIAAGQTEAFTKIVGQKSVMFLGVMIFGPIAALILKHTEKFWIHRIKSGFEMLVNNFYLAF" }, "paralog": false, "identity": 0.9285714285714286, "score": 122.0, "evalue": 4.08e-38, "description": "Internal stop codon at 771081. Nonsense mutation around 771081.", "psc": { "uniref90_id": "UniRef90_UPI001E56736E", "uniref50_id": "UniRef50_UPI001E56736E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E56736E", "UniRef:UniRef50_UPI001E56736E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E56736E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E56736E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5390.663499999998, "isoelectric_point": 9.102758598327636 }, "id": "CNOCLNGIAL_1369", "locus": "GCA000143945_01369" }, { "type": "cds", "sequence": "contig_1", "start": 771111, "stop": 772145, "strand": "+", "frame": 3, "gene": null, "product": "PTS mannitol transporter subunit IICB", "db_xrefs": [ "EC:2.7.1.197", "KEGG:K02800", "SO:0001217", "UniRef:UniRef50_UPI001F2174FA", "UniRef:UniRef90_UPI001F2174FA" ], "nt": "ATGCTGGTAAATAATTTTTACCTTGGCTTTTTAGGTTTCGCCTTAATATTTCCTAGTTTTTACCTATCAATTTACTTAATTGGTTACATTCAATTAGGTTTAAAACTGCTTGTTGAAATAATGCAACAGTACAAACTTTATCCAATAGCTGCCATTGTGATAGAGCCGGCCAAAGTTTTATTCCTAAATAATGCCATTAACCATGGCGTGCTAACTCCATTAGGCTTACAACAAGTAAGAGACAGTGGTAAATCAATTCTTTTCTTATTGGAATCAAATCCTGGTCCAGGTTTGGGTCTCCTTGTTGCTTTCTTAATCTTTTTCTTTAAACGTGATAAAAAGCTTTCTAGTAATGCGGCATCTTCTGGTCCTATTCACCTCTTTGGTGGTATCCATGAAGTTTACTTTCCGTTTGTGCTATTAAAACCTGTGTTAATTCTGGCCACAATTGCTGGTGGTGTTGTGGGAAACGGCATTTTGCAAATATTTAATGCTGGGACAATTGCACCAGTGTCACCTGGTTCTGTTATTGCTGGTTTTCTCCAAATTAATAAAACACCTTTAGATGTAGCTGGTTATGCTTTGGCACTCGTTTTATCAGCTGTAACTTCGTTGTTAATTTCGCTTTTATTACTTTCTTTAACACGTAAAAAACAATTGAAAACACTGCAAGAAGCGCAAGCACAAGTTGCAGAAATGAAGCAAACGCCTGCAAAAAAACCAAGACAAAAAGATACTCCAGCAATTGCTACAAAGATAGACTTTTCCCAAGTTACCTTTGTTTGCGATGCAGGTATGGGTTCTTCTACTATGGGAGCAGCTATATTTAGAAAAGAACTAAAAAATCAAAATATTGAAGACATTACAGTGATTAATAAAGCAATAGTTGATTTAAAAGATGAAAAGGTAATTATTACCATTTCCCAACTTTATGATCGCGTAAAAGCGAAGCGTGCAGACGCTACGATTTATACCATCAATCAGTTCTTAGATAAACAAGGCTATTTAACTATTATTGAAAAGATTAAAAAATGA", "aa": "MLVNNFYLGFLGFALIFPSFYLSIYLIGYIQLGLKLLVEIMQQYKLYPIAAIVIEPAKVLFLNNAINHGVLTPLGLQQVRDSGKSILFLLESNPGPGLGLLVAFLIFFFKRDKKLSSNAASSGPIHLFGGIHEVYFPFVLLKPVLILATIAGGVVGNGILQIFNAGTIAPVSPGSVIAGFLQINKTPLDVAGYALALVLSAVTSLLISLLLLSLTRKKQLKTLQEAQAQVAEMKQTPAKKPRQKDTPAIATKIDFSQVTFVCDAGMGSSTMGAAIFRKELKNQNIEDITVINKAIVDLKDEKVIITISQLYDRVKAKRADATIYTINQFLDKQGYLTIIEKIKK", "aa_hexdigest": "dfadc8c41de71dad2c0cb084e1920ad8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F2174FA", "query_cov": 0.997093023255814, "subject_cov": 0.8225419664268585, "identity": 1.0, "score": 632.0, "evalue": 4.18e-227, "valid": true, "product": "PTS mannitol transporter subunit IICB", "ec_ids": [ "2.7.1.197" ], "uniref50_id": "UniRef50_UPI001F2174FA", "kegg_orthology_id": "K02800" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2174FA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2174FA" ], "product": "PTS mannitol transporter subunit IICB" }, "genes": [], "id": "CNOCLNGIAL_1370", "locus": "GCA000143945_01370" }, { "type": "cds", "sequence": "contig_1", "start": 772142, "stop": 772345, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01232.29" ], "nt": "ATGAAAAGAATTAATGTATTGCACTTTGGCGCTGGTAATATAGGTAGAGGTGTTATATTACCGATTTACCAACAAAATGACTTTTCCATTGATTTAGTGGAATTGAATCAAAATACAGTTAATGAGTTACAAAAGCAAAAACAATACCAAGTTCATTACCTTGATTGTGATCAAAGTCAGTTAGTTAATGACTTTAATACTTGA", "aa": "MKRINVLHFGAGNIGRGVILPIYQQNDFSIDLVELNQNTVNELQKQKQYQVHYLDCDQSQLVNDFNT", "aa_hexdigest": "ddda0e9fd35972435ab7f37929199393", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01232.29", "name": "Mannitol_dh", "length": 62, "aa_cov": 0.9104477611940298, "hmm_cov": 1.0, "evalue": 1.0475558039748936e-21, "score": 73.26538848876953, "start": 5, "stop": 65 } ], "seq_stats": { "molecular_weight": 7779.6486, "isoelectric_point": 5.393133735656738 }, "id": "CNOCLNGIAL_1371", "locus": "GCA000143945_01371" }, { "type": "cds", "sequence": "contig_1", "start": 772379, "stop": 773149, "strand": "+", "frame": 2, "gene": "mtlD", "product": "mannitol-1-phosphate 5-dehydrogenase", "db_xrefs": [ "COG:COG0246", "COG:G", "EC:1.1.1.17", "GO:0005829", "GO:0008926", "GO:0019592", "KEGG:K00009", "SO:0001217", "UniRef:UniRef50_P78008", "UniRef:UniRef90_P78008" ], "nt": "ATGGAAAGAGCTGATGTTATTTCCACTTCAATAGGTGCTAAAAACTTAGCTAGTTTAAAAACTTTGTTTGACAAAGCTAAGTTCCATAAAAGGGCTATAGTGCTTTGCTTTGAAAACGGCTTTCGGATTAGTTCTAACTTTAAAAACATTTTGCAATTAAATAACACACAAGTAAATTTTGTGGATGTGGTAATCGATACAATTGCACCTAACTTCGAAAAGAAAGCGAATTTCTTGGATATTTATTGCGAGAAGTATTCAGAAATTTATGCTGAAACTTTCCCGCTAGAAATTAAGGGAGTAAATCAAACGAATAGTTTAGATAGATTCATTATTAAAAAACTTTTATTAGTCAATGCACTGCACAGTGTTATTGGACTTTTAGGCTTTCAACAAAAGCTTAAGTATGTACATGAAACATTGCAAGTTAAAAGTAATTTGACTTTTGTAGAAAAATTAGCCCAACAAATTATTGATGCCCTTTGTGCTGAATATCCTGAATTTAATAAAAATAATTTGTTAAGTTATGGCAAAAATAACTTAGTCCGTTTTGCTAATCCCAAGATTCAAGATTTAAACACACGCTTAATTAGGGAACCATTACGTAAACTTAACCAAAATGAACGCTTTTATGCCATTTATAAACTCTTTAAAAAGAATAAGATTGCACTAAATAATATTTTGCAAGTTTATCTGATGGTTTTAAAAACTAATATAACAGATGATACCGAAAGCCAACAAATTGCTAAATTAATAAATGAAAAAGCTTGA", "aa": "MERADVISTSIGAKNLASLKTLFDKAKFHKRAIVLCFENGFRISSNFKNILQLNNTQVNFVDVVIDTIAPNFEKKANFLDIYCEKYSEIYAETFPLEIKGVNQTNSLDRFIIKKLLLVNALHSVIGLLGFQQKLKYVHETLQVKSNLTFVEKLAQQIIDALCAEYPEFNKNNLLSYGKNNLVRFANPKIQDLNTRLIREPLRKLNQNERFYAIYKLFKKNKIALNNILQVYLMVLKTNITDDTESQQIAKLINEKA", "aa_hexdigest": "72981174081e762d6173b285a6177393", "start_type": "ATG", "rbs_motif": null, "genes": [ "mtlD" ], "truncated": "both", "pseudogene": { "start": 772142, "stop": 773236, "observations": { "mutation": [ 772343, 773147, 773189 ], "stop": [ 772343, 773147, 773189 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78008", "query_cov": 1.0, "subject_cov": 0.7032967032967034, "identity": 0.996, "score": 493.0, "evalue": 3.34e-174, "gene_start": 1, "gene_end": 256, "reference_start": 80, "reference_end": 335, "reference_sequence": "MKRINVLHFGAGNIGRGVILPIYQQNDFSIDLVELNQNTVNELQKQKQYQVHYLDCDQSQLVNDFNTWNLKDEAKIIELMERADVISTSIGAKNLASLKTLFDKAKFHKRAIVLCFENGFRISSNFKNILQLNNTQVNFVDVVIDTIAPNFEKKANFLDIYCEKYSEIYAETFPLEIKGVNQKNSLDRFIIKKLLLVNALHSVIGLLGFQQKLKYVHETLQVKSNLTFVEKLAQQIIDALCAEYPEFNKNNLLSYGKNNLVRFANPKIQDLNTRLIREPLRKLNQNERFYAIYKLFKKNKIALNNILQVYLMVLKTNITDDTESQQIAKLINEKAWTELAKLSSLEESEWNLIKQELSREITKK" }, "paralog": false, "identity": 0.989010989010989, "score": 697.0, "evalue": 3.22e-257, "description": "Internal stop codon at 772343, 773147, 773189. Nonsense mutation around 772343, 773147, 773189.", "psc": { "uniref90_id": "UniRef90_P78008", "gene": "mtlD", "product": "mannitol-1-phosphate 5-dehydrogenase", "ec_ids": [ "1.1.1.17" ], "uniref50_id": "UniRef50_P78008", "cog_id": "COG0246", "cog_category": "G", "kegg_orthology_id": "K00009", "go_ids": [ "GO:0005829", "GO:0008926", "GO:0019592" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78008", "EC:1.1.1.17", "UniRef:UniRef50_P78008", "COG:COG0246", "COG:G", "KEGG:K00009", "GO:0005829", "GO:0008926", "GO:0019592" ] }, "pscc": { "uniref50_id": "UniRef50_P78008", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78008" ], "product": "Mannitol-1-phosphate 5-dehydrogenase" } }, "id": "CNOCLNGIAL_1372", "locus": "GCA000143945_01372" }, { "type": "cds", "sequence": "contig_1", "start": 773217, "stop": 773648, "strand": "+", "frame": 3, "gene": "mtlF", "product": "Mannitol-specific phosphotransferase enzyme IIA component", "db_xrefs": [ "COG:COG4668", "COG:G", "GO:0005737", "GO:0005886", "GO:0009401", "GO:0016301", "GO:0090563", "RefSeq:WP_010875010.1", "SO:0001217", "UniParc:UPI000013295B", "UniRef:UniRef100_P75145", "UniRef:UniRef50_P75145", "UniRef:UniRef90_P75145" ], "nt": "GTGAAATTACTAAAAAATAACATTTACATTAACGTTTATTTAAAAAACAAACAAGAAATTTTTGAATTTGTTTTTAAAAAGTTTAAAGAAGATGGTGCTGTACTAGATAGTTTTTTACCAGCAATTGTGGAACGCGATAAAGCAGCTAGTGTAGCAATTGGTAATTACCTCTTTTTACCCCACCCAGTTTATGACGAAATAGCAAACATTCAAAAAGAAAAAATGGTGTTTATTGGTTTAAAAGATGTAATTAACATTGATGGTCAACCAATTAAATTTATCTGTGGTTTAGCCTTAAAAGGTGAGCACCAAATGGATGCGTTGCAATCTTTAGCAATAGCTTTTTCAGACCCTGAAGAAGTGGAAAAGTTAGTTAAAGATAAGGATTTAACACAAGATAAGGTACTTGAATTTTTAGCAAAACACAACTAA", "aa": "MKLLKNNIYINVYLKNKQEIFEFVFKKFKEDGAVLDSFLPAIVERDKAASVAIGNYLFLPHPVYDEIANIQKEKMVFIGLKDVINIDGQPIKFICGLALKGEHQMDALQSLAIAFSDPEEVEKLVKDKDLTQDKVLEFLAKHN", "aa_hexdigest": "c06514f8b7e06b0a390b0475ecc1436f", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013295B", "ncbi_nrp_id": "WP_010875010.1", "uniref100_id": "UniRef100_P75145", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013295B", "RefSeq:WP_010875010.1", "UniRef:UniRef100_P75145" ] }, "ips": { "uniref100_id": "UniRef100_P75145", "uniref90_id": "UniRef90_P75145" }, "psc": { "uniref90_id": "UniRef90_P75145", "gene": "mtlF", "product": "Mannitol-specific phosphotransferase enzyme IIA component", "uniref50_id": "UniRef50_P75145", "cog_id": "COG4668", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0005886", "GO:0016301", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75145", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75145" ], "product": "Mannitol-specific phosphotransferase enzyme IIA component" }, "genes": [ "mtlF" ], "id": "CNOCLNGIAL_1373", "locus": "GCA000143945_01373" }, { "type": "cds", "sequence": "contig_1", "start": 773816, "stop": 773998, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCTATACCTTTAATTGGCATGGACAGTATTCCAATAATGGCTTCACTGCCAAGAATGGTGATAAGAGTATTCTTTCGATCTTTCCCTTACTATTAAACCTAATCAAAAAACAATTTACAGCAGAAAACTTCATCGTTAAAAATGAAGAATTAAAAGCTGAAAAAATAGTAACAGAATGA", "aa": "MIYTFNWHGQYSNNGFTAKNGDKSILSIFPLLLNLIKKQFTAENFIVKNEELKAEKIVTE", "aa_hexdigest": "2728ab0456a88e75cb4813772de976f5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6950.919499999998, "isoelectric_point": 8.03174533843994 }, "id": "CNOCLNGIAL_1374", "locus": "GCA000143945_01374" }, { "type": "cds", "sequence": "contig_1", "start": 774155, "stop": 774409, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGTTAATGCGTAAAAAGAAACTTTTGTCAAGGATTTCCTTCGGTTCTTTGTTTTTGTTATGTGGAACAATATTAAGTGCTTGCACATGGATCCAAGCCAATCTTAGGAATCTTCTTAAAGAAACAACTGGCAAAGATTTTGATTTGTCTAAAGCAATTAAAATTCCGGAAGGTAGGCAAAACCTCATAAATTCTTTAAAGAAATCTTATGAAGTTAATCCAAAAGATACAACAAAGCTATTGTTAGACGCATGA", "aa": "MLMRKKKLLSRISFGSLFLLCGTILSACTWIQANLRNLLKETTGKDFDLSKAIKIPEGRQNLINSLKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "1b5af5852a8f105a0151a9168d3fd15f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 0.9761904761904762, "subject_cov": 0.8118811881188119, "identity": 0.8290000000000001, "score": 133.0, "evalue": 5.08e-39, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "CNOCLNGIAL_1375", "locus": "GCA000143945_01375" }, { "type": "cds", "sequence": "contig_1", "start": 774686, "stop": 775300, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_655", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6483", "UniRef:UniRef100_A0A0H3DML3", "UniRef:UniRef50_P75136", "UniRef:UniRef90_P75136" ], "nt": "ATGAGCAACAATAATAAATATTTCACTATAACTAAAAAACAGTACAAAAAAATGCGCCGCAATAAAATTGAACTTCTTTTTAATGTGAAAGTCCTAAAAAAGAAAAACGGTCGGCAAAAGTTCAAAATACTGCATGAAGTTGAGAATAAGCCAAAAATACCAATTAAAATAATTGAAGATCAACCGGAGTCGCCAAAGCCACCTAAAACTCCTAAACCCCCTAAACCGCCTAAAGGTCCTGACAATCCTGAGGAGCCGGATTCTCCAGAAGAACCCAAAGAAACAGATCAACCAGGTGGTCCCGATAATCCTAATGCAGGAAATAAAAAGATGCCAACACCTGAAGAATACGTAACTCGTAAAGAATTCAACGAATTCAAAGATTCAAACAATCAACGACTTACAAAGATTGAAAATAAGGTTGATAAGTTAGAAGTCAAAGTCGACAAATTAGCTGAAATTGTTCAAACTCAGGGCGAAGAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGTGAAACCCTACAGCTAATTTTGAAAACACTGCAAAAGATGAACGATCGTCTGGACAGAATGGAAACTCGCTTAGACAAGATCGATTCGCCCAAATAG", "aa": "MSNNNKYFTITKKQYKKMRRNKIELLFNVKVLKKKNGRQKFKILHEVENKPKIPIKIIEDQPESPKPPKTPKPPKPPKGPDNPEEPDSPEEPKETDQPGGPDNPNAGNKKMPTPEEYVTRKEFNEFKDSNNQRLTKIENKVDKLEVKVDKLAEIVQTQGEEIKELKVEQKAQGETLQLILKTLQKMNDRLDRMETRLDKIDSPK", "aa_hexdigest": "fff37ba2429770b9ee5da4c9d1bf9a3d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6483", "uniref100_id": "UniRef100_A0A0H3DML3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6483", "UniRef:UniRef100_A0A0H3DML3" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DML3", "uniref90_id": "UniRef90_P75136" }, "psc": { "uniref90_id": "UniRef90_P75136", "product": "UPF0134 protein MPN_655", "uniref50_id": "UniRef50_P75136" }, "pscc": { "uniref50_id": "UniRef50_P75136", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75136" ], "product": "UPF0134 protein MPN_655" }, "genes": [], "id": "CNOCLNGIAL_1376", "locus": "GCA000143945_01376" }, { "type": "cds", "sequence": "contig_1", "start": 775477, "stop": 775599, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DMA4", "UniRef:UniRef90_A0A0H3DMA4" ], "nt": "GTGTTAATTATGAAATATAAAAAGATAGGTGATATTAAATCTAAAGGATTTTTCGAATCTAAAGAAGAATATAAGGAACTTTTAAAAAAATGTGAACTAATTTCTTTTGAAGCTTTTTTCTGA", "aa": "MLIMKYKKIGDIKSKGFFESKEEYKELLKKCELISFEAFF", "aa_hexdigest": "b0af4350483516dd641f18005e3f2a64", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0H3DMA4", "query_cov": 1.0, "subject_cov": 0.9090909090909091, "identity": 1.0, "score": 79.3, "evalue": 5.59e-19, "valid": true, "uniref50_id": "UniRef50_A0A0H3DMA4" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DMA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DMA4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4840.760899999998, "isoelectric_point": 8.787959480285647 }, "id": "CNOCLNGIAL_1377", "locus": "GCA000143945_01377" }, { "type": "cds", "sequence": "contig_1", "start": 775775, "stop": 776095, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCGATACCCCAGGAGTCTTCTTTAAACGAATTGATGAAATGGCTGTTGGTTACAAGTTAGTTTTAACTAACGTTATTAAAAGGGAAGTAGTCCCTTTGGAAGATGTAGGTGCGTTTGCTTTTTGTTATTTAAGCAAACATTATCCGCAATTGTTACCATATGAAGGTACAGACTTCACTGAGTTTTTACATAAATTTGCTATCAGTCGTAAGTTGTTGCAAAAATCGAATCAGCTCAACATTAATTTAGCGTGCGAACTGTTTGTTAGTGAACTGATAAACGGGAAGTACGGTAAACTGAGTTTTGAGTTAGATTAG", "aa": "MSDTPGVFFKRIDEMAVGYKLVLTNVIKREVVPLEDVGAFAFCYLSKHYPQLLPYEGTDFTEFLHKFAISRKLLQKSNQLNINLACELFVSELINGKYGKLSFELD", "aa_hexdigest": "9ca259566b1ccafaf0ec49739a05e34a", "start_type": "TTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12155.996799999988, "isoelectric_point": 6.096573066711424 }, "id": "CNOCLNGIAL_1378", "locus": "GCA000143945_01378" }, { "type": "cds", "sequence": "contig_1", "start": 776120, "stop": 776590, "strand": "-", "frame": 2, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254", "SO:0001217", "UniRef:UniRef50_P75135", "UniRef:UniRef90_P75135" ], "nt": "ATGGATACATTTACCTCTGCAGTCAAGATTAACTGGTTTCCGGGTCACATGAAGAAGACCCATGACCAACTCAAAAAACTAGCTAGTAGTTTAGATGGTGTAATTGAGGTAGTTGATGCGCGTGCACCAACGTTAACCCAAAACCCCGAAATTACGGCTTACTTTACCAATAAACCGAAACTCACTTTAGCTTTAAAGGCTGATTTAGCACAAACAGTGGCTAACTCCAATATTTTATGGGGAACCTTAAAGCAAGGTTTGCAACTTAAACGCTTAGTTATTAAGAAACTGCAAACTTTGTTTCAAGCAAAAAAGAATCAGTTAAAAGCCAAAGGTTTATTAGTGCACCAGTTTCGGTTAGCGGTTATTGGAATGCCCAACGTTGGTAAATCCAGTTTAATTAACTTGTTACTAAATAAAAATCACTTACAGGTAGCTAATCGGGCTGGGGTTACCAAGTCAATGAGTTGA", "aa": "MDTFTSAVKINWFPGHMKKTHDQLKKLASSLDGVIEVVDARAPTLTQNPEITAYFTNKPKLTLALKADLAQTVANSNILWGTLKQGLQLKRLVIKKLQTLFQAKKNQLKAKGLLVHQFRLAVIGMPNVGKSSLINLLLNKNHLQVANRAGVTKSMS", "aa_hexdigest": "26aecbf46c5a5e62f936b20eae5dd902", "start_type": "ATG", "rbs_motif": null, "genes": [ "ylqF" ], "truncated": "5-prime", "pseudogene": { "start": 775817, "stop": 776590, "observations": { "mutation": [ 776122 ], "stop": [ 776122 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75135", "query_cov": 1.0, "subject_cov": 0.5756457564575646, "identity": 1.0, "score": 300.0, "evalue": 4.05e-101, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MDTFTSAVKINWFPGHMKKTHDQLKKLASSLDGVIEVVDARAPTLTQNPEITAYFTNKPKLTLALKADLAQTVANSNILWGTLKQGLQLKRLVIKKLQTLFQAKKNQLKAKGLLVHQFRLAVIGMPNVGKSSLINLLLNKNHLQVANRAGVTKSMSWNQISSEFYLSDTPGVFFKRIDEMAVGYKLVLTNVIKREVVPLEDVGAFAFCYLSKHYPQLLPYEGTDFTEFLHKFAISRKLLQKSNQLNINLACELFVSELINGKYGKLSFELD" }, "paralog": false, "identity": 0.9961089494163424, "score": 502.0, "evalue": 2.38e-183, "description": "Internal stop codon at 776122. Nonsense mutation around 776122.", "psc": { "uniref90_id": "UniRef90_P75135", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_P75135", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75135", "UniRef:UniRef50_P75135", "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75135", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75135" ], "product": "Probable ribosome biogenesis GTPase A" } }, "id": "CNOCLNGIAL_1379", "locus": "GCA000143945_01379" }, { "type": "cds", "sequence": "contig_1", "start": 776559, "stop": 776870, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "GTGTTATTTACGGGTACAGTAGTGAGTCACCTTTTCCCAAGGTATAACTTTGCTGAAATTAAGGTCTTTACTGACAAGATTGAAGAAGTGAGGTTGGCTCTGCTCAACGATAAAGCGACTCACAGTCTGTCCATTCAAGAAACGATGGGTGGATACTCATTAGCCAAAAAACGGATGATAGTTTCAGTAACTATGTATGTGGAAATTCCTAACCTCATCCGCATCATCCGGAAAATTGACAAAGATTGTTTAGTATCAATTACAAGGATCCGAGGGATCGATGGATACATTTACCTCTGCAGTCAAGATTAA", "aa": "MLFTGTVVSHLFPRYNFAEIKVFTDKIEEVRLALLNDKATHSLSIQETMGGYSLAKKRMIVSVTMYVEIPNLIRIIRKIDKDCLVSITRIRGIDGYIYLCSQD", "aa_hexdigest": "a292284b1356122b0f948962f7ed6b44", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 52, "aa_cov": 0.5048543689320388, "hmm_cov": 1.0, "evalue": 2.4748324414006055e-11, "score": 38.781734466552734, "start": 44, "stop": 95 } ], "seq_stats": { "molecular_weight": 11829.831199999986, "isoelectric_point": 8.974917793273928 }, "id": "CNOCLNGIAL_1380", "locus": "GCA000143945_01380" }, { "type": "cds", "sequence": "contig_1", "start": 776919, "stop": 777104, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGATCTTGTGCTGGGGGTGCCGATTTCCTAACGCAATACTTTGCGAGAAAGAAAAACCGCTCCGTGGGGCCAATTCTCTTTTATGTCAATACCTTTATTTTGATCATTGCGATCTTAATGGGTTCGTTTGTCGCTGGCAGTATTGTGCTCCAAGATATTCCCGATTACAAAAAAAGTGCTTGA", "aa": "MGSCAGGADFLTQYFARKKNRSVGPILFYVNTFILIIAILMGSFVAGSIVLQDIPDYKKSA", "aa_hexdigest": "0644a8e2ac08d27f410a46c58827d58e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6618.7403, "isoelectric_point": 9.35966510772705 }, "id": "CNOCLNGIAL_1381", "locus": "GCA000143945_01381" }, { "type": "cds", "sequence": "contig_1", "start": 777201, "stop": 777668, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTGCCCTTTATGAGGCTAAAAAACCTTTAAAGTACACAATTGTCTACATCCTGGCTTTAGTGAACGCCTTCTTTTTGCTGGTGTTTATCCAACAAACGGGATTGTACAGCTTTGGGATTAGTTCGCTAACCCAAGGTTTTGCGCGTTTACTGTTTGTCTTGTTGAAGAACCTCGAAGATGGACAACGCAACCTAGTGTTCAACATCTTTTACTGGCTGTTTTATGTAATAGTCAACATTCCCCTGATTATTTTTTCTTACAAAAAAATTGGGAAACGTTTCACCATTCTGTCAACCCATTACGTGGTCGCTTCCAACGTCTTTGGTTTTATCTTTAGTATTATTCCGGGGGCTAATCAATTGCCATCGATGTTGTCCGCAGTGCACCACACTGAATTTTGGGAAGCTGCAAAAAAAGCTGAAGGTGTTGATCAAAGCGCGTTATTTGTACCGTTCTTATGA", "aa": "MFSALYEAKKPLKYTIVYILALVNAFFLLVFIQQTGLYSFGISSLTQGFARLLFVLLKNLEDGQRNLVFNIFYWLFYVIVNIPLIIFSYKKIGKRFTILSTHYVVASNVFGFIFSIIPGANQLPSMLSAVHHTEFWEAAKKAEGVDQSALFVPFL", "aa_hexdigest": "91321d7a6c83d5dd4d0d5bdd0788acb5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17692.718899999985, "isoelectric_point": 9.487441444396975 }, "id": "CNOCLNGIAL_1382", "locus": "GCA000143945_01382" }, { "type": "cds", "sequence": "contig_1", "start": 777780, "stop": 778139, "strand": "-", "frame": 1, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_010875015.1", "SO:0001217", "UniParc:UPI0000133CB4", "UniRef:UniRef100_P75133", "UniRef:UniRef50_Q8EWU9", "UniRef:UniRef90_P75133" ], "nt": "ATGAAAAAAATAAATAAACAAGCTTTAATAGATTTAGTAGAACAAAAGCAATTAAAGGCTTATGTGCCTGAGTTTAGCGCTGGTGACGAAGTGAATGTTGCCATCAAACTTAAAGAAAAGGAAAAGGTCAGAATCCAAAACTTTACGGGAACTGTCCTACGCCGTCGTGGTAAAGGTATTAGTGAAACCTTCATTGTGCGTAAAACCACTGATGGTATTCCGATTGAAAAAAACTTCCAAATTCATAACCCTAATATTTCAATTGAATTGAAAAGACGGGGTAAAGTAAGACGCGCTTACATTTCATATATGCGTGAGCGCTCTGGTAAAGCTGCCAAAATTAAAGAGCGAAAACAATAG", "aa": "MKKINKQALIDLVEQKQLKAYVPEFSAGDEVNVAIKLKEKEKVRIQNFTGTVLRRRGKGISETFIVRKTTDGIPIEKNFQIHNPNISIELKRRGKVRRAYISYMRERSGKAAKIKERKQ", "aa_hexdigest": "5d27fe9483784a60add336e9224bdb23", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133CB4", "ncbi_nrp_id": "WP_010875015.1", "uniref100_id": "UniRef100_P75133", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133CB4", "RefSeq:WP_010875015.1", "UniRef:UniRef100_P75133" ] }, "ips": { "uniref100_id": "UniRef100_P75133", "uniref90_id": "UniRef90_P75133" }, "psc": { "uniref90_id": "UniRef90_P75133", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_Q8EWU9", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EWU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EWU9" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "CNOCLNGIAL_1383", "locus": "GCA000143945_01383" }, { "type": "cds", "sequence": "contig_1", "start": 778123, "stop": 778665, "strand": "-", "frame": 3, "gene": null, "product": "tRNA (guanine-N(1)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "nt": "ATGGCTTACGGAGGAGGTGGCGGGATGATTCTAAAAGCTGAACCAATTCTCAAAGCTTTAAAGGCTTGTAGAACTCCACAATCGAAAGTAGTTTTATTATCACCTGAGGGACAACAGTTTTCCCAACCCATGGCGCAAGCTTTAACCCAAACGGAACACCTCATTTTGATCTGTGGTCACTATGAGGGCTTTGATTACCGCTTGTATAAACATGTCGATCAAATTATTTCGTTAGGTGACTTTGTGTTGAGTGGTGGGGAGTTAGTGGCTTTAAGTGTGATTGATGCCACTGTTCGTTTAATTAAGGGTGTCATTAATGATCAGAGCTTAATCCACGAATCATTTAACAACTACTTACTGGATTTCCCCGCTTACACTAGGCCATATGATCTAGACGGGGATAAGGTACCTGAAATATTATTATCAGGTGACCATAAAAAGATAGAGGCTTATCGCAAAGAACAACAACTGCTCAGAACGGCTCAATACCGTCCTGATTTGTATAAGCAATATTTAGCAAAGAAAGATGAAAAAAATAAATAA", "aa": "MAYGGGGGMILKAEPILKALKACRTPQSKVVLLSPEGQQFSQPMAQALTQTEHLILICGHYEGFDYRLYKHVDQIISLGDFVLSGGELVALSVIDATVRLIKGVINDQSLIHESFNNYLLDFPAYTRPYDLDGDKVPEILLSGDHKKIEAYRKEQQLLRTAQYRPDLYKQYLAKKDEKNK", "aa_hexdigest": "bbba8a27843b964f59f5fd9a127acf63", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A534NV99", "query_cov": 0.9444444444444444, "subject_cov": 0.821256038647343, "identity": 0.535, "score": 171.0, "evalue": 6.91e-51, "valid": false, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_B1GZ20", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_B1GZ20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [], "id": "CNOCLNGIAL_1384", "locus": "GCA000143945_01384" }, { "type": "cds", "sequence": "contig_1", "start": 778932, "stop": 779081, "strand": "-", "frame": 1, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627", "SO:0001217", "UniRef:UniRef50_P47684", "UniRef:UniRef90_P75131" ], "nt": "ATGGGTCGTGTTCACTACCCAACCTACAGAATAGTAGCTGTTGATTCCAGAGTTAAACGTGATGGTAAGTACATTGCGTTAATTGGCCATCTCAATCCTGCATTAAAAGAAAATAAGTGCAAGATTGATGAAGCTGTGGCCTTGGAATGA", "aa": "MGRVHYPTYRIVAVDSRVKRDGKYIALIGHLNPALKENKCKIDEAVALE", "aa_hexdigest": "637d190f7323f0450db83dfbabd2a9be", "start_type": "ATG", "rbs_motif": "AAAAA", "genes": [ "rpsP" ], "truncated": "5-prime", "pseudogene": { "start": 778821, "stop": 779105, "observations": { "mutation": [ 778934 ], "stop": [ 778934 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75131", "query_cov": 1.0, "subject_cov": 0.5212765957446809, "identity": 1.0, "score": 102.0, "evalue": 1.49e-27, "gene_start": 1, "gene_end": 49, "reference_start": 9, "reference_end": 57, "reference_sequence": "MVKIRLMRMGRVHYPTYRIVAVDSRVKRDGKYIALIGHLNPALKENKCKIDEAVALEWLNKGAKPTDTVRSLFSQTGLWKKFVESKKKPVAKSK" }, "paralog": false, "identity": 0.9787234042553191, "score": 184.0, "evalue": 2.78e-62, "description": "Internal stop codon at 778934. Nonsense mutation around 778934.", "psc": { "uniref90_id": "UniRef90_P75131", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_P47684", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75131", "UniRef:UniRef50_P47684", "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47684" ], "product": "Small ribosomal subunit protein bS16" } }, "id": "CNOCLNGIAL_1385", "locus": "GCA000143945_01385" }, { "type": "cds", "sequence": "contig_1", "start": 779169, "stop": 779393, "strand": "-", "frame": 1, "gene": "norM", "product": "putative MG447-like protein", "db_xrefs": [ "COG:COG0534", "COG:V", "GO:0005886", "GO:0015297", "GO:0042910", "RefSeq:WP_243679576.1", "SO:0001217", "UniParc:UPI000AC194D4", "UniRef:UniRef100_P75130", "UniRef:UniRef50_P47685", "UniRef:UniRef90_P75130" ], "nt": "ATGCAAGGGGTTATTGTTTTTGTACCCTTGTTGTTTATCTTTCAAGCAATTACCTTACAAACAAAAAACATTGAAATCTTTATCTGGTTATTAACGGCTAACGCTGCTCTGGCTGGATTGATTAACGTTTTGATTGGCCAAGTACACATCCACTTCTTTATGGATAAATACTTCGCTCAAAAACATAAGAGTCGGATTGTCCAGTTCATTGAGCGCTATTCTTAG", "aa": "MQGVIVFVPLLFIFQAITLQTKNIEIFIWLLTANAALAGLINVLIGQVHIHFFMDKYFAQKHKSRIVQFIERYS", "aa_hexdigest": "3b5ab56965889da8b0b63069a76e0208", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000AC194D4", "ncbi_nrp_id": "WP_243679576.1", "uniref100_id": "UniRef100_P75130", "db_xrefs": [ "SO:0001217", "UniParc:UPI000AC194D4", "RefSeq:WP_243679576.1", "UniRef:UniRef100_P75130" ] }, "ips": { "uniref100_id": "UniRef100_P75130", "uniref90_id": "UniRef90_P75130" }, "psc": { "uniref90_id": "UniRef90_P75130", "gene": "NorM", "product": "Uncharacterized protein MG447 homolog", "uniref50_id": "UniRef50_P47685", "cog_id": "COG0534", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015297", "GO:0042910" ] }, "pscc": { "uniref50_id": "UniRef50_P47685", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47685" ], "product": "Uncharacterized protein MG447" }, "genes": [ "norM" ], "id": "CNOCLNGIAL_1386", "locus": "GCA000143945_01386" }, { "type": "cds", "sequence": "contig_1", "start": 779406, "stop": 780818, "strand": "-", "frame": 1, "gene": "norM", "product": "putative MG447-like protein", "db_xrefs": [ "COG:COG0534", "COG:V", "GO:0005886", "GO:0015297", "GO:0042910", "SO:0001217", "UniRef:UniRef50_P47685", "UniRef:UniRef90_P75130" ], "nt": "GTGAATAGCCAACAAGATCGTCTAGCGAAGCAAGTCCTCATCCAAAAATCCTTTGAATCGAAAAGACTGTTTCTAACGATCTTGCGCTTTGCTATTCCGACTTTTTTCTTTGCGCTGTTTTCCGCTGCTTATGTCTTTGTGGACCAAATTATGGTGATTAAGTTTGTCCCGCATGGACCACTTAATCCCGATTCAATCTTTACTGACCAGGCCTTAATTGAGGAATTTAAAGCATCCGCGTTTTATAAGGGTGGGGACATTCCCAACCACACGGAACTAACAGCTAGTCAATTAGTCAAAACAGTCTTAAATATTAGCCAGCCAATTGTAGTCATTCTCAACGCCATTACCATCTTTGTGCCGTTGGGTACGGGGGTGATCTTTTCCAAAACCATTGGTAAGGGTGATGAGAAAAAGATTAAGGATGCTTGGAATACCGGGTTAGTTTCCACTACCTTATTTGCCCTAGTGACCCAAATTATTGTGCTAGCGATTGCCAAAGAGTGGTTGCAGTTTAACTTGGATAAAGTCGATGAACAACACCACGTGCAAGTAGCGGATCAGTTTCAACACTTCTTTAACGAAAAGGCGGTTGCGATTGGTTCAGAGTACGTTTACATTTTGATTGGCTTTAACATCATACCGATGTTGAGCCGCTTGTTTTTTTACTTAGGCCAATCAGAGGGCCGGCAACTGTTTATTGCAATTGTGCCTCCTTTATCGAACTTACTGAACGTCCTGTTTGTTTTCTTGTTAGTGCGTTTTTCCACTTTAGGGGTTGTGGGCTCCGCGGTGGCTGCGATTTTGGTTTACTTCATTACCTTTATGGCTTATGTGGTGTACCTTATTAGTTTAAATAAACGTGGTTTAACCTACCTTTCGTTGAGGGACTTTTCCTTTAAACGGGTTAGTTTTAACCTCTTTTTGGTCATTTCAATGGTGGGGTTAGCCTCCTTTTTCCGTAATGGTTCGCTCTCAATTCTCAACACCTTTTACGAAAGCTTTTTGGTGAATTTGACCAAAACGCTTACGGACCAAAGTGATACCTTTTACTTGGTGTTACTAACGGGACCCATTGCAATTGCTAACCTAACTTCAGCCGCCATCTTTGGTGTTCTCCAAGGGGTGCGGACAGTGGTATCCTACAAGTTTGGTCAGGGCCAGTTGGCTGACATTAAAAGGATTAATGTTTATACGTTGTTAGTGTGTTTGGTATTTGCTGCTTTGCTGTATTTAATTCTGGCAGTTGGATTGGGCAAAGAAATCCTAGTGCACCTGTTTGATACAAGTGCAGCTACCTTAATGCTCGCTAATCAGTTTTCCTTAATTGTGCAAGCGCAAGTGTTCTTTGTGGCCATTGGCGCTACCAGTCAACAGTACTTCCAAAATACCAACCGGGTGTTGTATTCGTGA", "aa": "MNSQQDRLAKQVLIQKSFESKRLFLTILRFAIPTFFFALFSAAYVFVDQIMVIKFVPHGPLNPDSIFTDQALIEEFKASAFYKGGDIPNHTELTASQLVKTVLNISQPIVVILNAITIFVPLGTGVIFSKTIGKGDEKKIKDAWNTGLVSTTLFALVTQIIVLAIAKEWLQFNLDKVDEQHHVQVADQFQHFFNEKAVAIGSEYVYILIGFNIIPMLSRLFFYLGQSEGRQLFIAIVPPLSNLLNVLFVFLLVRFSTLGVVGSAVAAILVYFITFMAYVVYLISLNKRGLTYLSLRDFSFKRVSFNLFLVISMVGLASFFRNGSLSILNTFYESFLVNLTKTLTDQSDTFYLVLLTGPIAIANLTSAAIFGVLQGVRTVVSYKFGQGQLADIKRINVYTLLVCLVFAALLYLILAVGLGKEILVHLFDTSAATLMLANQFSLIVQAQVFFVAIGATSQQYFQNTNRVLYS", "aa_hexdigest": "839e66ae9cb97e64f5585f52f3ebfbf1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75130", "query_cov": 1.0, "subject_cov": 0.8561020036429873, "identity": 0.996, "score": 823.0, "evalue": 5.06e-298, "valid": true, "gene": "NorM", "product": "Uncharacterized protein MG447 homolog", "uniref50_id": "UniRef50_P47685", "cog_id": "COG0534", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015297", "GO:0042910" ] }, "pscc": { "uniref50_id": "UniRef50_P47685", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47685" ], "product": "Uncharacterized protein MG447" }, "genes": [ "norM" ], "id": "CNOCLNGIAL_1387", "locus": "GCA000143945_01387" }, { "type": "cds", "sequence": "contig_1", "start": 780819, "stop": 781007, "strand": "-", "frame": 1, "gene": null, "product": "peptide-methionine (R)-S-oxide reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024947FD0" ], "nt": "ATGATCCGCACCGAAGTACGCGCCAAAAACTCCAACAGCCACCTAGGGCACGTCTTTCGTGATGGTCCCGAAGAGCATGGTGGTTTGCGTTACTGCATTAACTCCGCTGCTCTAAAGTTCATTCCGTTTGCGGAAATGGAATCGGCGGGGTATGGTGAATACCTCAAATTATTTAAGGAATCTAACTAA", "aa": "MIRTEVRAKNSNSHLGHVFRDGPEEHGGLRYCINSAALKFIPFAEMESAGYGEYLKLFKESN", "aa_hexdigest": "280923d08d9886123ebc2193d66ab6d1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024947FD0", "query_cov": 0.9193548387096774, "subject_cov": 0.8769230769230769, "identity": 0.7190000000000001, "score": 93.2, "evalue": 7.58e-24, "valid": false, "product": "peptide-methionine (R)-S-oxide reductase", "uniref50_id": "UniRef50_UPI0024947FD0" }, "pscc": { "uniref50_id": "UniRef50_UPI0024947FD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024947FD0" ], "product": "peptide-methionine (R)-S-oxide reductase" }, "genes": [], "id": "CNOCLNGIAL_1388", "locus": "GCA000143945_01388" }, { "type": "cds", "sequence": "contig_1", "start": 781071, "stop": 781274, "strand": "-", "frame": 1, "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "db_xrefs": [ "COG:COG0229", "COG:O", "EC:1.8.4.12", "GO:0005737", "GO:0006979", "GO:0030091", "GO:0033743", "SO:0001217", "UniRef:UniRef50_Q9CJ17", "UniRef:UniRef90_P75129" ], "nt": "ATGAGTAAGTACCAAAAAAAGAGTGACGGGGAACTAAAGCGGACCTTAACTAAACTACAGTACGACGTTACCCAAAACGCACACACCGAACCGCCCTATACTAATGAATATAACCGCCATTACGAAAAGGGTATTTACGTTGACATTACGTCCGGTGAACCGTTGTTTATCTCAACTGATAAGTTCAAATCCGGTTGTGGTTGA", "aa": "MSKYQKKSDGELKRTLTKLQYDVTQNAHTEPPYTNEYNRHYEKGIYVDITSGEPLFISTDKFKSGCG", "aa_hexdigest": "e6168f0acf4796d952af691d466aa850", "start_type": "ATG", "rbs_motif": null, "genes": [ "msrB" ], "truncated": "5-prime", "pseudogene": { "start": 780819, "stop": 781274, "observations": { "mutation": [ 781073 ], "stop": [ 781073 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75129", "query_cov": 1.0, "subject_cov": 0.44370860927152317, "identity": 1.0, "score": 143.0, "evalue": 1.77e-42, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MSKYQKKSDGELKRTLTKLQYDVTQNAHTEPPYTNEYNRHYEKGIYVDITSGEPLFISTDKFKSGCGWPAFTKPISQDLIANYRDESHGMIRTEVRAKNSNSHLGHVFRDGPEEHGGLRYCINSAALKFIPFAEMESAGYGEYLKLFKESN" }, "paralog": false, "identity": 0.9933774834437086, "score": 311.0, "evalue": 2.47e-111, "description": "Internal stop codon at 781073. Nonsense mutation around 781073.", "psc": { "uniref90_id": "UniRef90_P75129", "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "ec_ids": [ "1.8.4.12" ], "uniref50_id": "UniRef50_Q9CJ17", "cog_id": "COG0229", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0033743", "GO:0030091", "GO:0006979" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75129", "EC:1.8.4.12", "UniRef:UniRef50_Q9CJ17", "COG:COG0229", "COG:O", "GO:0005737", "GO:0033743", "GO:0030091", "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_Q9CJ17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9CJ17" ], "product": "Peptide methionine sulfoxide reductase MsrB" } }, "id": "CNOCLNGIAL_1389", "locus": "GCA000143945_01389" }, { "type": "cds", "sequence": "contig_1", "start": 781271, "stop": 781942, "strand": "-", "frame": 2, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "db_xrefs": [ "COG:COG0073", "COG:J", "GO:0000049", "SO:0001217", "UniRef:UniRef50_P47687", "UniRef:UniRef90_P75128" ], "nt": "ATGCGTTACGGTTTTTTTGATATTTCTCAGGACTTTATCACCATTTTTTGTCCCAAGAAAACACTTAAGAACTGTATGTTTGGTTTAATTGGTAGCCGCACGCAAGCTACTTTGCGCCAAGAAAAGAACCAGAACTTTAGCTTCTTTGTCAATGAAGCCAATGAGATTGCTGGGTTTAACTTCTTTGACATTAAAAAGAGCTTTCGCAGGGGATTAATTTCCCATCACTTTACCGCAGGGTTGAATTACCCTTCTTTGAAGTTGGTCAAAAAGATTAGTGAGCTGTTGAACTATGATTTAACACCCTTAGCCAAAAAGGTACCCTTTGTGGTTTGTGAGGTCATTTCGGCCATTCCGATTCCCAACACTCATTTGAAAAGGTGTAAGGTCAACACTGGTTCCAATAAGTCCTTGGATGTCGTGTGTGGGGCGGATAATGTTCGGGTTGGCCTTAAAACTGTTTTAGTCCATGTTGGTGGTGTTTTACCTGATGGCACGATTATCAAGAAGGCTAAAATTGCGGGGTATGATTCCATGGGAATGCTCTGTTCGGAAAAAGAGCTCCATTTAAAACCTAAAAATCAGGGTATAATTGAAATTAAGTCGCACATCAAAATAGGCAAAAGTTTCCTTGATGTTTACTTAAATAATAGTGAAAAATTCAGTGCATGA", "aa": "MRYGFFDISQDFITIFCPKKTLKNCMFGLIGSRTQATLRQEKNQNFSFFVNEANEIAGFNFFDIKKSFRRGLISHHFTAGLNYPSLKLVKKISELLNYDLTPLAKKVPFVVCEVISAIPIPNTHLKRCKVNTGSNKSLDVVCGADNVRVGLKTVLVHVGGVLPDGTIIKKAKIAGYDSMGMLCSEKELHLKPKNQGIIEIKSHIKIGKSFLDVYLNNSEKFSA", "aa_hexdigest": "93a2d687b612a5aaf80bb0ed3450344b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75128", "query_cov": 1.0, "subject_cov": 0.9529914529914529, "identity": 0.996, "score": 447.0, "evalue": 5.38e-159, "valid": true, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "uniref50_id": "UniRef50_P47687", "cog_id": "COG0073", "cog_category": "J", "go_ids": [ "GO:0000049" ] }, "pscc": { "uniref50_id": "UniRef50_P47687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47687" ], "product": "Uncharacterized protein MG449" }, "genes": [ "ytpR" ], "id": "CNOCLNGIAL_1390", "locus": "GCA000143945_01390" }, { "type": "cds", "sequence": "contig_1", "start": 781949, "stop": 782821, "strand": "-", "frame": 2, "gene": "degV", "product": "DegV domain-containing MG450-like protein", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "RefSeq:WP_014575051.1", "SO:0001217", "UniParc:UPI0001DD6489", "UniRef:UniRef100_A0AAV5N7U0", "UniRef:UniRef50_P47688", "UniRef:UniRef90_P75127" ], "nt": "GTGAGAGTCGCATTCTTAGTTGACTCTGTTAGTAACCTCAAGGAAAGCCAGGCACAACATCTGTATGTGCTACCGCTTTTCATCATTGAATCAACTCCTGAAGCTGAAACCACCTTTAGTTCGGGGTTTGACATTGATTTAAAAACCCTCACGGACAAGATGGCTAACGCTCCGAAGGGAGTGAAGTTCTCCACATCGCAAACTACTGAAGAAATAGTGCGCGCTAAGGTGAGTGAACTAGTGGAGCAGTATGACCTGATTATCGGCATTCCGATTGATAAGGAAATCTCCGCTTCGTACGCGAACTGGAAGTTAGTTGAAAAGGATTACGCTTCGAAGTTCCATGTATTGGATGCCAGAGCGGTGGAAATGATCATTGATTGGCTAATCAATGACATTAAGGGTTGGTTGAATACCAATCCTTATTCCAGGGAAGGCTTGGATCGCTTTGTGGAGCAGTACCGCAAGAAAACGGCCGCAGTGTTGTTTGTCACTGATACCAAACCACTAGTGGCAGGCGGGCGGCTTTCCAATCTTAAATCCTTTATTATCAAGAGCTTAAAGTTTCACTTACTGATTAGTTTTTTAGGTGAAAATGGTAAGTTACAGTTCTTTGATAAAGCGCGTTCGACTCATGATGCGCATAAGCTAGCAGTCAAGTTTTTAAAGAAACAGCTGTTAAAACACTCCGCTAAACTGAAACGGGGTGCCTTTCTTACCACCGTCTTTGACGAACAGACCAACACTAATTTAGTCCAAGAGTTTGATAAACTGTTGGATCATTCCATTAATATAGAACAGAGCTTACTGTCCCCGGTCATTTGTACCCACACGGGATTGAACAGCTATGTAATTGTGCTGCAAGGCGAATAG", "aa": "MRVAFLVDSVSNLKESQAQHLYVLPLFIIESTPEAETTFSSGFDIDLKTLTDKMANAPKGVKFSTSQTTEEIVRAKVSELVEQYDLIIGIPIDKEISASYANWKLVEKDYASKFHVLDARAVEMIIDWLINDIKGWLNTNPYSREGLDRFVEQYRKKTAAVLFVTDTKPLVAGGRLSNLKSFIIKSLKFHLLISFLGENGKLQFFDKARSTHDAHKLAVKFLKKQLLKHSAKLKRGAFLTTVFDEQTNTNLVQEFDKLLDHSINIEQSLLSPVICTHTGLNSYVIVLQGE", "aa_hexdigest": "dcda69a3c57f3cc450f1ec1172b476f9", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6489", "ncbi_nrp_id": "WP_014575051.1", "uniref100_id": "UniRef100_A0AAV5N7U0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6489", "RefSeq:WP_014575051.1", "UniRef:UniRef100_A0AAV5N7U0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N7U0", "uniref90_id": "UniRef90_P75127" }, "psc": { "uniref90_id": "UniRef90_P75127", "gene": "DegV", "product": "DegV domain-containing protein MG450 homolog", "uniref50_id": "UniRef50_P47688", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P47688", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47688" ], "product": "DegV domain-containing protein MG450" }, "genes": [ "degV" ], "id": "CNOCLNGIAL_1391", "locus": "GCA000143945_01391" }, { "type": "cds", "sequence": "contig_1", "start": 782821, "stop": 784005, "strand": "-", "frame": 3, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "COG:COG0050", "COG:J", "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020", "RefSeq:WP_010875022.1", "SO:0001217", "UniParc:UPI0000129DB8", "UniRef:UniRef100_P23568", "UniRef:UniRef50_P23568", "UniRef:UniRef90_P23568" ], "nt": "ATGGCAAGAGAGAAATTTGACCGATCTAAACCCCACGTTAATGTAGGTACTATTGGCCACATTGACCACGGTAAAACAACTTTAACAGCAGCTATTTGTACTGTATTAGCAAAAGAAGGTAAATCAGCTGCTACTCGTTACGACCAAATCGATAAGGCTCCGGAAGAAAAAGCACGGGGAATTACGATTAACTCCGCTCACGTGGAGTACTCCTCTGACAAGCGTCACTATGCTCACGTTGACTGTCCAGGACACGCTGACTACATTAAGAACATGATTACTGGTGCTGCACAAATGGATGGTGCCATTCTAGTAGTTTCAGCAACTGACAGTGTTATGCCCCAAACCCGTGAACACATTTTGTTGGCCCGCCAAGTGGGTGTGCCACGCATGGTAGTGTTCCTAAACAAGTGTGACATTGCAACTGATGAAGAAGTGCAAGAGTTAGTAGCAGAAGAGGTACGTGACTTATTAACTTCTTACGGCTTTGATGGCAAGAACACCCCTATTATTTATGGTTCTGCACTTAAAGCGCTTGAAGGTGATCCTAAGTGGGAAGCTAAGATCCATGATTTAATGAATGCAGTTGATGAATGGATTCCAACTCCTGAACGTGAAGTGGACAAACCCTTCTTGTTGGCAATCGAAGACACCATGACGATTACTGGCCGTGGTACCGTGGTTACCGGTCGGGTTGAACGTGGTGAATTGAAAGTAGGTCAAGAAATTGAAATCGTTGGTTTACGTCCAATCCGTAAAGCAGTTGTTACCGGAATCGAAATGTTCAAAAAGGAACTTGATTCAGCAATGGCTGGGGACAACGCTGGGGTATTACTCCGTGGTGTGGACCGTAAAGAAGTGGAACGTGGTCAAGTGTTAGCTAAACCAGGTTCGATTAAACCGCACAAGAAATTTAAAGCGGAAATCTATGCTTTAAAGAAGGAAGAAGGTGGTCGTCACACCGGTTTCTTAAACGGTTACCGTCCCCAATTCTACTTCCGTACTACAGACGTTACTGGTTCGATTTCCCTACCAGAAAACACCGAAATGGTGCTACCAGGTGACAATACCTCGATTACAGTTGAACTAATTGCACCAATTGCTTGTGAAAAAGGTAGTAAGTTCTCCATCCGTGAAGGTGGTCGAACGGTTGGTGCTGGTTCAGTCACGGAAGTGCTTGAATAG", "aa": "MAREKFDRSKPHVNVGTIGHIDHGKTTLTAAICTVLAKEGKSAATRYDQIDKAPEEKARGITINSAHVEYSSDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSATDSVMPQTREHILLARQVGVPRMVVFLNKCDIATDEEVQELVAEEVRDLLTSYGFDGKNTPIIYGSALKALEGDPKWEAKIHDLMNAVDEWIPTPEREVDKPFLLAIEDTMTITGRGTVVTGRVERGELKVGQEIEIVGLRPIRKAVVTGIEMFKKELDSAMAGDNAGVLLRGVDRKEVERGQVLAKPGSIKPHKKFKAEIYALKKEEGGRHTGFLNGYRPQFYFRTTDVTGSISLPENTEMVLPGDNTSITVELIAPIACEKGSKFSIREGGRTVGAGSVTEVLE", "aa_hexdigest": "7ebf3457ab79c849138f493d5b5ed8d0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129DB8", "ncbi_nrp_id": "WP_010875022.1", "uniref100_id": "UniRef100_P23568", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129DB8", "RefSeq:WP_010875022.1", "UniRef:UniRef100_P23568" ] }, "ips": { "uniref100_id": "UniRef100_P23568", "uniref90_id": "UniRef90_P23568" }, "psc": { "uniref90_id": "UniRef90_P23568", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_P23568", "cog_id": "COG0050", "cog_category": "J", "go_ids": [ "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P23568", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P23568" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "CNOCLNGIAL_1392", "locus": "GCA000143945_01392" }, { "type": "cds", "sequence": "contig_1", "start": 784156, "stop": 784551, "strand": "+", "frame": 1, "gene": null, "product": "putative MG452-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75125", "UniRef:UniRef90_P75125" ], "nt": "TTGGGGGTAATTCCCTTATTTTTTATTTGTTTTGTTTTTGTAATTGCTGACATTGTCATCAGTTTGCAAAATAAAGGCCACATTATAGAAGAGATAGATAAGTTTACTAACCAGAGCAATGTTATGTTGCTCATTTATGCATGTTGGTATGTAAGCAAACCAAAGAGCCATTATCTCAAGAACCAGCAGTTCTTTTTGAGTGCCTTTGCTTACATCATCTTTACTTTTTTAGGCTACAACGTCATTTTGGCGGCTTCACAACAAGCTTACAGTGATAAGGATGCTTATTCCTTGGCAAGTTCTGTCTTTCTCCATGTGTTGGCACCAATTGCTTTCTTGGTTGCAGGTATAGTTAAGATGAAAACGGACAAGGATGTAACCTTTAATCATTTTTGA", "aa": "MGVIPLFFICFVFVIADIVISLQNKGHIIEEIDKFTNQSNVMLLIYACWYVSKPKSHYLKNQQFFLSAFAYIIFTFLGYNVILAASQQAYSDKDAYSLASSVFLHVLAPIAFLVAGIVKMKTDKDVTFNHF", "aa_hexdigest": "45aa88c1ce0d70b2c955d8260f249865", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 784090, "stop": 784845, "observations": { "mutation": [ 784120, 784549, 784711 ], "stop": [ 784120, 784549, 784711 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75125", "query_cov": 1.0, "subject_cov": 0.5219123505976095, "identity": 0.992, "score": 255.0, "evalue": 3.3e-84, "gene_start": 1, "gene_end": 131, "reference_start": 23, "reference_end": 153, "reference_sequence": "MEQNNCKIRTWFAKLALAQKVFLGVIPLFFICFVFVIADIVISLQNKGHIIEEIDKFTNQSNVMLLIYACWYVSKPKSHYLKNQQFFLSAFAYIIFTFLGYNVILAASQQAYSDKDAYSLASSVFLHVLAPIAFLVAGIVKMKTDKDVTFNHFWKSLGYFMIYPLVYGLYLATIPYVRGHYVSDDGKSTTYVVYGEITNTKDNPIVAWPVVICFLFIYFPLSFLAVYALQCKLLNRPLKAQFKCATNKCPK" }, "paralog": false, "identity": 0.9880478087649402, "score": 497.0, "evalue": 5.85e-182, "description": "Internal stop codon at 784120, 784549, 784711. Nonsense mutation around 784120, 784549, 784711.", "psc": { "uniref90_id": "UniRef90_P75125", "product": "Uncharacterized protein MG452 homolog", "uniref50_id": "UniRef50_P75125", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75125", "UniRef:UniRef50_P75125", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75125", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75125" ], "product": "Uncharacterized protein MG452 homolog" } }, "id": "CNOCLNGIAL_1393", "locus": "GCA000143945_01393" }, { "type": "cds", "sequence": "contig_1", "start": 784570, "stop": 784713, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTATCCCTTAGTGTATGGGTTGTACTTAGCGACCATTCCTTATGTAAGGGGTCATTATGTGAGTGATGATGGTAAGTCTACTACCTATGTGGTTTATGGGGAGATTACCAACACCAAGGATAACCCGATCGTCGCTTGA", "aa": "MIYPLVYGLYLATIPYVRGHYVSDDGKSTTYVVYGEITNTKDNPIVA", "aa_hexdigest": "785f9afb9451ddad465ab75d45e2a03a", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5271.944600000002, "isoelectric_point": 5.4105833053588865 }, "id": "CNOCLNGIAL_1394", "locus": "GCA000143945_01394" }, { "type": "cds", "sequence": "contig_1", "start": 784833, "stop": 785369, "strand": "+", "frame": 3, "gene": null, "product": "UTP--glucose-1-phosphate uridylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0M420" ], "nt": "ATGCCCAAAATAAGAAAAGCCGTTATTCCCGCAGCCGGACTGGGTACCAGATTACTCCCAGCCACTAAGGCCATTCCCAAAGAGATGTTGCCCTTAGTCAATAAACCCACAATTCAGTACATTGTGGAAGAAGCAGTAGCGAGTGGCATTAAGGAAATCCTAGTAATTGTGTCTTCTAAAAAGGAAGCGATTATTGACCACTTTGACTATGACTTTATCTTGGAAAATGCTTTACTCCAAAAGCACAAAGATCAAGAACACCAAGAAATTAAGGATATTGCCAACTTAGCGCACATTTACTTTGTCCGCCAAAAACACCAACACGGTTTAGGAGATGCCATTTTGCACGCCAAGTCGTTTGTTGGTAACGAAGACTTTGCCGTCCTTTTAGGTGATGATGTCGTGTTTGGTGAACAACCGGCATTAGCACAATGTATTCAAGCTTATGAACAAACAGATTGTCAGGTAATAGGCGTGCAAGAAGTGCCCCATGACCAGGTCAACAAGTATGGCATAGTAACCCCTGAAGCTAATTGA", "aa": "MPKIRKAVIPAAGLGTRLLPATKAIPKEMLPLVNKPTIQYIVEEAVASGIKEILVIVSSKKEAIIDHFDYDFILENALLQKHKDQEHQEIKDIANLAHIYFVRQKHQHGLGDAILHAKSFVGNEDFAVLLGDDVVFGEQPALAQCIQAYEQTDCQVIGVQEVPHDQVNKYGIVTPEAN", "aa_hexdigest": "80fdc0b8a704ba45efd13621b614de99", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0020C167D8", "query_cov": 0.9831460674157303, "subject_cov": 0.9259259259259259, "identity": 0.589, "score": 199.0, "evalue": 2.2e-62, "valid": false, "product": "UTP--glucose-1-phosphate uridylyltransferase", "ec_ids": [ "2.7.7.9" ], "uniref50_id": "UniRef50_A0A3D0M420", "kegg_orthology_id": "K00963" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0M420", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0M420" ], "product": "UTP--glucose-1-phosphate uridylyltransferase" }, "genes": [], "id": "CNOCLNGIAL_1395", "locus": "GCA000143945_01395" }, { "type": "cds", "sequence": "contig_1", "start": 785400, "stop": 785708, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGAAAAACCAGCTGTCAACGAAGCCAAGAGCAACCTAGCCATTTTGAGCCGTTACATCCTTAAACCCAGTATTTTTACAGCTCTTAAACAAGTACCGTTTGGTGTTGGAGGTGAGTTACAGTTGACTGATGGGTTGAACTATTGTTTACAGCAAGGGGAACCCTTCTTTGCCAAGCACTTTGGTGGTACCCGGTTTGATGTCGGTACTAAAAATGGTTTTATTAAAGCGAACCTGTACACGGCTTTAAAAACCGATGCCATTACTAAGGATGAAGTCTTGGCCATCCTCAAGGAATTTGTTTAA", "aa": "MVEKPAVNEAKSNLAILSRYILKPSIFTALKQVPFGVGGELQLTDGLNYCLQQGEPFFAKHFGGTRFDVGTKNGFIKANLYTALKTDAITKDEVLAILKEFV", "aa_hexdigest": "8e0e761a25a077d24bf11a4bec7e16df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11209.897899999985, "isoelectric_point": 8.733677101135253 }, "id": "CNOCLNGIAL_1396", "locus": "GCA000143945_01396" }, { "type": "cds", "sequence": "contig_1", "start": 785718, "stop": 786140, "strand": "-", "frame": 1, "gene": "osmC", "product": "Organic hydroperoxide resistance protein-like", "db_xrefs": [ "COG:COG1764", "COG:V", "EC:1.11.1.28", "GO:0006979", "KEGG:K04063", "RefSeq:WP_010875025.1", "SO:0001217", "UniParc:UPI0000139829", "UniRef:UniRef100_P75123", "UniRef:UniRef50_P47692", "UniRef:UniRef90_P75123" ], "nt": "ATGGCAGTAATTTACAAAACAACAGCACACGCTAGCGCCGGCAGGGAAGGCGTTGTCCAAACCGTTGATGGTTTTACCGTTAGTTTAGCATTTCCTAAACCTGGTGCTACTCACCAGGACAAAAACAACCCCGAACAGCTGTTTGCTTCAGCTTATGCTGGTTGTTTTTCGCAAGCTGTGCGTGTGGTTTTACAACAGCACCAACTGCAATTAGCTACTCAACCTATTGTGGGTGTTAGTGTCGAACTGCATGACCAGGACGGTCTGTTCCACATTAAAGCCGGTGTTGAATTAGCGATTACGGGCGTGGACCAAACCACAGCGCAAACAGTCATTACGGCAGCGCATGCCATGTGTCCGTTTAGCCGTTTAATTAAACCAGAAAACTTCTTGGGTTTAACCTTGAACGGTGCCAAGCTTTAA", "aa": "MAVIYKTTAHASAGREGVVQTVDGFTVSLAFPKPGATHQDKNNPEQLFASAYAGCFSQAVRVVLQQHQLQLATQPIVGVSVELHDQDGLFHIKAGVELAITGVDQTTAQTVITAAHAMCPFSRLIKPENFLGLTLNGAKL", "aa_hexdigest": "b94a5980c1a9ce9401afd5a7f15cdac8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139829", "ncbi_nrp_id": "WP_010875025.1", "uniref100_id": "UniRef100_P75123", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139829", "RefSeq:WP_010875025.1", "UniRef:UniRef100_P75123" ] }, "ips": { "uniref100_id": "UniRef100_P75123", "uniref90_id": "UniRef90_P75123" }, "psc": { "uniref90_id": "UniRef90_P75123", "gene": "OsmC", "product": "Organic hydroperoxide resistance protein-like", "ec_ids": [ "1.11.1.28" ], "uniref50_id": "UniRef50_P47692", "cog_id": "COG1764", "cog_category": "V", "kegg_orthology_id": "K04063", "go_ids": [ "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_P47692", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47692" ], "product": "Organic hydroperoxide resistance protein-like" }, "genes": [ "osmC" ], "id": "CNOCLNGIAL_1397", "locus": "GCA000143945_01397" }, { "type": "cds", "sequence": "contig_1", "start": 786166, "stop": 787365, "strand": "+", "frame": 1, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "db_xrefs": [ "COG:COG0162", "COG:J", "EC:6.1.1.1", "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437", "KEGG:K01866", "RefSeq:WP_014325672.1", "SO:0001217", "UniParc:UPI0001DD648B", "UniRef:UniRef100_A0AAV5NAV1", "UniRef:UniRef50_P47693", "UniRef:UniRef90_P75122" ], "nt": "ATGACAAAAGATCTCCTAGCACTTTTAAAAGAACGGGGCTTATTTGTACAAGCTAACTTTGAAAAGGAATTAAAGCAATTATTAAACCAAGGATCATTTGCTTTCTATGTTGGCTTTGACCCCACAGCACCCTCACTCCATATTGGCAACTATGTGCTGCTCCATGTAGCACAAATCTTCCAAGCAATGGGTCATATTCCCCACGTCTTGTTGGGTAGTGGGACGGCTTTAATAGGTGATCCTACTGGCAGAATGGAACTGCGCCAGATGATGAGTCGTGAAACAATTGCCGAAAATACGCGCAACATTAAAAAACAAATTAGGCGCTTTTTGGGTAGTAATGTAGTGTTTTGCCAAAACGAGACCTGGTTAAAGAAACTCAATTACATTGAGGTCATCAGGGAATTGGGACCCTGCTTTTCGGTCAACAAGATGTTAGCGACCGATGCCTTTAGTGCTCGTTGGGAACGCGGTTTAACGTTGATGGAATTGAACTACATGGTATTACAGGCCTATGACTTCTATTATTTAAACCAAAAGTATGGGGTGCAACTGCAAATAGGGGGCAGTGACCAGTGGGCTAATATCCTAGCGGGGGCTGATTTAATTAGACGCAAAACCCAAAAACAGGTGTACGGCATGACCACTAACTTATTAGTGAAAGCTAATGGGGAAAAGATGGGTAAGAGTGCTAGTGGAGCTTTATGGTTAGATCCACAAAAGACCAGTCCGTATGACTTCTACCAGTATTGGATTAACCTTGATGATGCTAGTTTGCAAAAGGTTTTCCTTATGCTCACTAAATTAGATACCAAGGCAATTGAAACCCTCTGCAACTTGAAGGGCGCTGCCATTAAGGAGGCCAAGGCAAAACTAGCCTTCGAGTTAACTGACGCTATTCATGGTACCAAGGCAGCTTTAGCCGCCCAAGCGAAAAGTGCCCGAATCTTTGCCTTTCAACCTGATACTGAAACCAAAACCGTGCGAGCTGGCACCAGGTTAGTGGATGTGATTGTTGATTTAGGTTTAGTTGTATCCAGATCAGAAGCGCGCCGGGTGATTCAGCAAGGTGGTTTAACGATTAACCAAGAAAAAGTGACTGATGTGGAAATGGTCCTACAAGCGAGTTCCCAACCACTAGTGATTGGTAAGGGCAAAAAGCGTTTTGTAACGGTGCAAGTGATAGCTAACACCAAATAA", "aa": "MTKDLLALLKERGLFVQANFEKELKQLLNQGSFAFYVGFDPTAPSLHIGNYVLLHVAQIFQAMGHIPHVLLGSGTALIGDPTGRMELRQMMSRETIAENTRNIKKQIRRFLGSNVVFCQNETWLKKLNYIEVIRELGPCFSVNKMLATDAFSARWERGLTLMELNYMVLQAYDFYYLNQKYGVQLQIGGSDQWANILAGADLIRRKTQKQVYGMTTNLLVKANGEKMGKSASGALWLDPQKTSPYDFYQYWINLDDASLQKVFLMLTKLDTKAIETLCNLKGAAIKEAKAKLAFELTDAIHGTKAALAAQAKSARIFAFQPDTETKTVRAGTRLVDVIVDLGLVVSRSEARRVIQQGGLTINQEKVTDVEMVLQASSQPLVIGKGKKRFVTVQVIANTK", "aa_hexdigest": "1cb0a501a91970a1ead4204c400ce684", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD648B", "ncbi_nrp_id": "WP_014325672.1", "uniref100_id": "UniRef100_A0AAV5NAV1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648B", "RefSeq:WP_014325672.1", "UniRef:UniRef100_A0AAV5NAV1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NAV1", "uniref90_id": "UniRef90_P75122" }, "psc": { "uniref90_id": "UniRef90_P75122", "gene": "tyrS", "product": "tyrosine--tRNA ligase", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_P47693", "cog_id": "COG0162", "cog_category": "J", "kegg_orthology_id": "K01866", "go_ids": [ "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437" ] }, "pscc": { "uniref50_id": "UniRef50_P47693", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47693" ], "product": "Tyrosine--tRNA ligase" }, "genes": [ "tyrS" ], "id": "CNOCLNGIAL_1398", "locus": "GCA000143945_01398" }, { "type": "cds", "sequence": "contig_1", "start": 787405, "stop": 788442, "strand": "-", "frame": 3, "gene": null, "product": "putative MG456-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010875027.1", "SO:0001217", "UniParc:UPI0000139830", "UniRef:UniRef100_P75121", "UniRef:UniRef50_P47694", "UniRef:UniRef90_P75121" ], "nt": "ATGCCAGAGCTAACTCGTTTTCAAAAGTTCTTTTTAACACCTGAAAAGTTCAATAAATTTACAAGAGTTGTTGGTTTTTGTGGTGTCTTTGCTTTAATTGCCCTTTCTTTAGGGATTTACAGCTATGTTGGTCAGGGTTCAATTGTTCCTAAAGTGGCAGCTCTTTTCCTAATTGCCTTAGGCGGGTTTACCTTGCTGTTGAGCTTTGTGATTAACTTTGTAGCTTTGTACAAACGCAGTCAGTTAATTCATTTGGTCAACCGTCAAGATCGTACTGATTTGTGGTTACAAAAGATGGCAAACAACAAGCAGTTTGAGCAATTTGAATTGTTTGAAAAAGGTCCAATTAGTGCGGATATTCTACCGACTTTTTACCCTGCTACCATCTATAACTTTGAGTTAGTTCCCAAGCAATTTAAGGTACAGTACAAGAATGGTCAAACGCTTAACTTTGCTAAGTTAAGTGCCATTAAGAGAAGTACTTCCAAAAATGAAAAGGTGGCTTGCTTAGTCGCTATTATTGATGCTGTTAGTGACCAACACTGGTTCTTAACTAAAAGCGATTTCCCCTTAATTAACACTGGTTTTTACGAATCTTTAACTGAAAGTAACCGCCAGAACAACGTTTTGCTCTACACTGAAAAAGACGCTAGTTTTAACTTTAATCAGTTAGATAAGGAGATGATAAAGCAGGTGTTGTTTAACCCGGTTAATGTGTATGCTAACTTTAACGTTTACAACAATACCACACACACTTACCTCATGATGAGTGTACCGATTACCTTCATGGATACTTCTTTAAGAATGGAGGAAGCTGTGGGTGATTTAGAACTCAATATTACCCGTCAAGCTGGTTATGACGCTGCTACCTTAGATTCATTCCATAAGGTAGTGGAACTGTTAAAGACCAAACTCATTGGTGATTTCAACAACGCTGAAACTACCAGTGCAACAGAAACAACAGTTGTGGCTGAAGTTACTGAACCCACAACCAATTCCAAGCGCAAACCAGTTAAAGCGAAGAAGGCTAAGAAATAA", "aa": "MPELTRFQKFFLTPEKFNKFTRVVGFCGVFALIALSLGIYSYVGQGSIVPKVAALFLIALGGFTLLLSFVINFVALYKRSQLIHLVNRQDRTDLWLQKMANNKQFEQFELFEKGPISADILPTFYPATIYNFELVPKQFKVQYKNGQTLNFAKLSAIKRSTSKNEKVACLVAIIDAVSDQHWFLTKSDFPLINTGFYESLTESNRQNNVLLYTEKDASFNFNQLDKEMIKQVLFNPVNVYANFNVYNNTTHTYLMMSVPITFMDTSLRMEEAVGDLELNITRQAGYDAATLDSFHKVVELLKTKLIGDFNNAETTSATETTVVAEVTEPTTNSKRKPVKAKKAKK", "aa_hexdigest": "5c92a724c8cf4ee52878013d9ef9b424", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139830", "ncbi_nrp_id": "WP_010875027.1", "uniref100_id": "UniRef100_P75121", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139830", "RefSeq:WP_010875027.1", "UniRef:UniRef100_P75121" ] }, "ips": { "uniref100_id": "UniRef100_P75121", "uniref90_id": "UniRef90_P75121" }, "psc": { "uniref90_id": "UniRef90_P75121", "product": "Uncharacterized protein MG456 homolog", "uniref50_id": "UniRef50_P47694", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47694", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47694" ], "product": "Uncharacterized protein MG456" }, "genes": [], "id": "CNOCLNGIAL_1399", "locus": "GCA000143945_01399" }, { "type": "cds", "sequence": "contig_1", "start": 788430, "stop": 789011, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014575052.1", "SO:0001217", "UniParc:UPI0001DD648C", "UniRef:UniRef100_A0AAX0SQR6", "UniRef:UniRef50_A0AAX0SQR6", "UniRef:UniRef90_A0AAX0SQR6" ], "nt": "GTGAAACAAACAGTTAAGTTTGCTAGTTTTGTCGATCAGCTCCTAGTTAACCTCGCGCACTTGCAAAAGCAGCTCAACCACTTGCAACCCGATTTGGGCACCTATAGCTTTCAGTGTTTTACCACCCTCAACGATGTTGGTAAAACCCCGCAAAACTTTATTGCAGTCTTAAAGCAACACTCCAACTTGTTGGGTTGTGAAACCAATGCTGTGCTAACTTGGTGGGAGGCTGTAGAATACCTGTTGTTAGAAGAAAACAAGGATGTGATTACCGTGGTGAAAACCCTTGTTAAACACACCGAAAAATTGATACAAACTAAAGCAATTACTTTAGGTCACAAACGGGTGTGGGATTTGTTGTATTACTTTTGTAAAAGCTTCTTGCAATTAGTAAACCGCACCAAAAGGCAGTACAAGCAGCTCGATGGGGTGTTAGTTGAACTGTATGAACGGCTTTGTCAAACCCGGGCGCAGTTTGGCAAGGCGCAGTTTTTAGGGAAACGTTCTGTAGGGCAAACAACTCCAGCGTTGTGTTTAACTTATCTTTTCTTACAATCTTTTATATACGCATGCCAGAGCTAA", "aa": "MKQTVKFASFVDQLLVNLAHLQKQLNHLQPDLGTYSFQCFTTLNDVGKTPQNFIAVLKQHSNLLGCETNAVLTWWEAVEYLLLEENKDVITVVKTLVKHTEKLIQTKAITLGHKRVWDLLYYFCKSFLQLVNRTKRQYKQLDGVLVELYERLCQTRAQFGKAQFLGKRSVGQTTPALCLTYLFLQSFIYACQS", "aa_hexdigest": "79a6a66b652dc4e83b473ee39b988084", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD648C", "ncbi_nrp_id": "WP_014575052.1", "uniref100_id": "UniRef100_A0AAX0SQR6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648C", "RefSeq:WP_014575052.1", "UniRef:UniRef100_A0AAX0SQR6" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQR6", "uniref90_id": "UniRef90_A0AAX0SQR6" }, "psc": { "uniref90_id": "UniRef90_A0AAX0SQR6", "uniref50_id": "UniRef50_A0AAX0SQR6" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SQR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SQR6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22313.758100000003, "isoelectric_point": 9.203845024108887 }, "id": "CNOCLNGIAL_1400", "locus": "GCA000143945_01400" }, { "type": "cds", "sequence": "contig_1", "start": 788995, "stop": 790452, "strand": "-", "frame": 3, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357JCK8" ], "nt": "GTGAGGTTTGACAACATTGCGGGACTCCAAGAAGAAAAACACGAGTTACTGGAAATTGTTGATTACCTCAAAAACCCCTTGAAGTATGCCCAAATGGGCGCTCGTTCCCCACGCGGTGTTATTCTTTACGGCCCTCCTGGTACGGGTAAAACGCTGTTAGCGAAAGCTGTTGCTGGTGAAGCTGGTGTGCCTTTCTTCCAGTCCACGGGTTCTGGCTTTGAAGATATGCTGGTTGGTGTGGGCGCCAAACGGGTACGGGACTTGTTCAATAAGGCTAAGAAAGCGGCCCCATGTATTATCTTCATCGATGAAATTGACTCGGTTGGTTCTAAACGGGGTCGCGTGGAGTTGTCCTCCTACTCGGTGGTCGAACAAACCTTAAACCAACTGTTAGCGGAAATGGATGGGTTTACTAGCCGCACTGGTGTTGTGGTGATGGCTGCTACCAACCGCTTGGATGTCCTTGATGATGCGTTATTACGCCCAGGTCGGTTTGATCGTCACATCCAGATTAACCTTCCCGACATTAAGGAACGCGAAGGCATCTTACAAGTGCACGCCAAGAACAAGAACTTGTCTTCCAAGATTAGCTTATTGGATGTTGCTAAACGTACCCCTGGTTTTAGTGGGGCTCAATTGGAAAACGTCATTAATGAAGCTACCCTGTTAGCAGTCCGTGATAACCGGACCACGATCAACATGAACGACATTGATGAAGCCATTGACCGGGTCATAGCTGGTCCTGCCAAAAAATCTCGGGTGGTCAGTGATGCTGATCGTAAACTAGTGGCCTACCACGAAGCAGGTCATGCTTTGGTTGGCCTCCATGTTCACAGTAACGATGAAGTGCAAAAGATTACCATTATTCCCCGGGGTCAAGCGGGTGGTTATACCTTGTCAACCCCTAAGAGTGGCGATCTCAATTTAAAGCGTAAATCTGACTTGTTAGCGATGATTGCTACAGCCATGGGCGGCCGTGCTGCAGAGGAAGAAATTTACGGTCCCCTAGAAATTACCACTGGCGCTTCCTCGGACTTTTATAAGGCGACCAATATTGCCCGTGCCATGGTTACCCAGTTAGGAATGTCCAAGTTAGGGCAAGTGCAATATGTACCTAGTCAAGGTACCGTCCCACCAGGTACCAAACTGTTCTCTGAGCAAACAGCAAAGGATATTGACTTTGAGATTAACGCCATTATTGAAGAACAGTACAAGAAGGCACGTACCATTATTAAAACGAACCGTAAGGAGTTGGAATTGTTAGTTGAAGCCTTGTTGATTGCCGAAACCATCTTAAAGAGTGACATCGATTACATTCACGAACACACTAAACTCCCTCCAGAGATCCTAGCGCAAAAGCAAGAACAGCAAGCCAAACAAAAGGCAGAAGCCAAGGAAGCTAAGTTAAATAAGAAGACTGAGAAGGATACAGAAAAAGACAGTGAAACAAACAGTTAA", "aa": "MRFDNIAGLQEEKHELLEIVDYLKNPLKYAQMGARSPRGVILYGPPGTGKTLLAKAVAGEAGVPFFQSTGSGFEDMLVGVGAKRVRDLFNKAKKAAPCIIFIDEIDSVGSKRGRVELSSYSVVEQTLNQLLAEMDGFTSRTGVVVMAATNRLDVLDDALLRPGRFDRHIQINLPDIKEREGILQVHAKNKNLSSKISLLDVAKRTPGFSGAQLENVINEATLLAVRDNRTTINMNDIDEAIDRVIAGPAKKSRVVSDADRKLVAYHEAGHALVGLHVHSNDEVQKITIIPRGQAGGYTLSTPKSGDLNLKRKSDLLAMIATAMGGRAAEEEIYGPLEITTGASSDFYKATNIARAMVTQLGMSKLGQVQYVPSQGTVPPGTKLFSEQTAKDIDFEINAIIEEQYKKARTIIKTNRKELELLVEALLIAETILKSDIDYIHEHTKLPPEILAQKQEQQAKQKAEAKEAKLNKKTEKDTEKDSETNS", "aa_hexdigest": "16d13bd606ae783af3b7d49c732ab51c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5RJK9", "query_cov": 0.9237113402061856, "subject_cov": 0.8327137546468402, "identity": 0.5579999999999999, "score": 473.0, "evalue": 3.3e-160, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A357JCK8", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A357JCK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357JCK8" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "CNOCLNGIAL_1401", "locus": "GCA000143945_01401" }, { "type": "cds", "sequence": "contig_1", "start": 790546, "stop": 791037, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTATCCTAGCCATTATTATTGGTATTTTGGTTTATATCCTAATGCCAAGGGCTACCACGGCTGTAATTGAAAAGTGGGAGCTTAGTGGAACTACACTTTCGGCTCAAATCAAAGGCTTATCAGGCAAACACACCTTCCAAAGAATTAATAATTCCACCTATGTCACCGATGACATTCTGCAGGTTTCCATTAGTTTCCAAGGGATAAATCCCATAGTAGTAACTGCCCACAAAGCTACTAATGGTAGTGGGGAAACTATCTTTAATATAGCAAACCTCTCGATTAATCAGTCAACTGGAAAGGCCATAGTTAATGGAATGATGACCCAAGACCAAAAAAGTAACAATGGCACTGAATTAGCTTCTATTAAAGGTCTCCATGACATCGGTACCTTTGTTGCTCCTGATACGAGAGCACGTGATGTGCTCAACATCTTCTTTGGCCTACTACCAATTATTATCTTTGTCATTTTCTTCTTACTCTTTTGA", "aa": "MVILAIIIGILVYILMPRATTAVIEKWELSGTTLSAQIKGLSGKHTFQRINNSTYVTDDILQVSISFQGINPIVVTAHKATNGSGETIFNIANLSINQSTGKAIVNGMMTQDQKSNNGTELASIKGLHDIGTFVAPDTRARDVLNIFFGLLPIIIFVIFFLLF", "aa_hexdigest": "fe17ecc576f14c70095f23a16dbcdfbf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17709.454599999994, "isoelectric_point": 8.068298912048338 }, "id": "CNOCLNGIAL_1402", "locus": "GCA000143945_01402" }, { "type": "cds", "sequence": "contig_1", "start": 791111, "stop": 791284, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648D", "UniRef:UniRef100_A0AB38W6Q2", "UniRef:UniRef50_A0AB38W6Q2", "UniRef:UniRef90_A0AB38W6Q2" ], "nt": "ATGCGATGGTTTAGTGTGTTGGTTTCCCTGGTAAAAAAGCACAAAAAAATCATCTTAATTACCTTTCTAACTTTGGCTGGTTTTGCAGCAGTGGGTGTATTAGTTACCCTTAGTGTTGTTAGAATAAAAACCAGTCAAATCACCCAACAAACAACAACAAATGAAAAAAAATAA", "aa": "MRWFSVLVSLVKKHKKIILITFLTLAGFAAVGVLVTLSVVRIKTSQITQQTTTNEKK", "aa_hexdigest": "b69880654c72a7dc3444d60a213cf24e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD648D", "uniref100_id": "UniRef100_A0AB38W6Q2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648D", "UniRef:UniRef100_A0AB38W6Q2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6Q2", "uniref90_id": "UniRef90_A0AB38W6Q2" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6Q2", "uniref50_id": "UniRef50_A0AB38W6Q2" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6Q2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6386.6795, "isoelectric_point": 11.30106601715088 }, "id": "CNOCLNGIAL_1403", "locus": "GCA000143945_01403" }, { "type": "cds", "sequence": "contig_1", "start": 791305, "stop": 791832, "strand": "-", "frame": 3, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0634", "COG:F", "EC:2.4.2.8", "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657", "KEGG:K00760", "RefSeq:WP_014325673.1", "SO:0001217", "UniParc:UPI0001DD648E", "UniRef:UniRef100_A0AAV5N6V8", "UniRef:UniRef50_P47696", "UniRef:UniRef90_P75119" ], "nt": "ATGGGCATTAAATCTATCATCATTGATCAAAAACAGGTTGAAGCGGGTTGTAACGCTGCGCTCAAATGGTGTAATGAACATTTTGCTGGTAAACAGGTCATAGTATTAGGGATCTTGAAGGGTTGTATTCCCTTTTTGGGCAAACTTATTAGTCAGTTTACCTTTGATTTACAACTGGACTTTGTGGCAGTCGCTTCTTACCATGGTGGCAGTCGCCAACAAGAAGCACCGAAGATTGTCCTAGATATGTCCCATGACCCTAAAGGCAAGGACATTCTTTTAATAGAAGACATAATCGACAGTGGTCGTTCTATTAAATTAGTATTGGACTTACTCCACACCCGAAAGGCCAAAAGTGTGATCTTAGTGAGCTTCATTGAAAAGCTCAAACCCAGGGAAGCTGACATTAAGGTGGACTATTCTTGCTTTAAAAACCAAGACGAATTCTTGGTTGGTTTTGGGTTAGACTACCAGGGCTTTTATCGCAATTTACCCTATGTTGGTGTCTTTGATCCTGAAGATAATTAA", "aa": "MGIKSIIIDQKQVEAGCNAALKWCNEHFAGKQVIVLGILKGCIPFLGKLISQFTFDLQLDFVAVASYHGGSRQQEAPKIVLDMSHDPKGKDILLIEDIIDSGRSIKLVLDLLHTRKAKSVILVSFIEKLKPREADIKVDYSCFKNQDEFLVGFGLDYQGFYRNLPYVGVFDPEDN", "aa_hexdigest": "b7160731d2072f4fbc2daf194a054ec1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD648E", "ncbi_nrp_id": "WP_014325673.1", "uniref100_id": "UniRef100_A0AAV5N6V8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648E", "RefSeq:WP_014325673.1", "UniRef:UniRef100_A0AAV5N6V8" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N6V8", "uniref90_id": "UniRef90_P75119" }, "psc": { "uniref90_id": "UniRef90_P75119", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_P47696", "cog_id": "COG0634", "cog_category": "F", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657" ] }, "pscc": { "uniref50_id": "UniRef50_P47696", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47696" ], "product": "Hypoxanthine-guanine phosphoribosyltransferase" }, "genes": [ "hpt" ], "id": "CNOCLNGIAL_1404", "locus": "GCA000143945_01404" }, { "type": "cds", "sequence": "contig_1", "start": 791832, "stop": 792257, "strand": "-", "frame": 1, "gene": "ispF", "product": "putative MG459-like protein", "db_xrefs": [ "COG:COG0245", "COG:I", "GO:0008685", "GO:0016114", "SO:0001217", "UniRef:UniRef50_Q49436", "UniRef:UniRef90_P75118" ], "nt": "GTGGAAATAGAAGATTCCAAGTACATTTATGACCACGATGCTAGTGATGACGCCAGTGACGTTATCCAGCTAGCAGTAGCTGATGCTTTATTTGGTGCTACTGCCTTAGGTGATGGACAAATTGTCTTCAATAAAGAAAAAACCCAGATCCCTTTAAAGGGTAATCCGCGTAAAGAAGCGCCCCGTATTTTGGCACGGACTTACAACTTTATTCGCAAGAACTGGTACATCAATAACATTGATATCACCTTAGAAATTCCCTCACAACAAAAGATGGATGACTATAAGCATGCCATCTTTGACTTTATTTGTACGGCTTTACGGATTACCGAACTGACAATTAACCTCAAGGTACGTGAACCGTTGAATTCGAATGAAATTAGTTGTTTAGCGGTCGTGTTAGTGGAACGACAAAGATTAAAGTAA", "aa": "MEIEDSKYIYDHDASDDASDVIQLAVADALFGATALGDGQIVFNKEKTQIPLKGNPRKEAPRILARTYNFIRKNWYINNIDITLEIPSQQKMDDYKHAIFDFICTALRITELTINLKVREPLNSNEISCLAVVLVERQRLK", "aa_hexdigest": "c6853fe3a877d1c2bec26b1e72d9a64f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75118", "query_cov": 1.0, "subject_cov": 0.834319526627219, "identity": 0.986, "score": 275.0, "evalue": 2.68e-93, "valid": true, "gene": "IspF", "product": "Uncharacterized protein MG459 homolog", "uniref50_id": "UniRef50_Q49436", "cog_id": "COG0245", "cog_category": "I", "go_ids": [ "GO:0008685", "GO:0016114" ] }, "pscc": { "uniref50_id": "UniRef50_Q49436", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49436" ], "product": "Uncharacterized protein MG459" }, "genes": [ "ispF" ], "id": "CNOCLNGIAL_1405", "locus": "GCA000143945_01405" }, { "type": "cds", "sequence": "contig_1", "start": 792459, "stop": 793451, "strand": "+", "frame": 3, "gene": "ldh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "EC:1.1.1.27", "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006096", "GO:0016020", "KEGG:K00016", "SO:0001217", "UniParc:UPI0001DD6490", "UniRef:UniRef100_P78007", "UniRef:UniRef50_P78007", "UniRef:UniRef90_P78007" ], "nt": "ATGATTGGTGGCTTAAACCAAATTAAAATGAAGGCAAGCTTGAATAAATTAGTTATGAAGAGTCTTAAAGTAGCACTCATTGGTTCTGGTGCAGTGGGCACCAGCTTTCTCTACGCTGCTATGAGCCGTGGTTTAGCGAGTGAATACATGGTTATCGACATTAACGAAAAATCCCAAGTAGGGAATGTTTTTGACCTCCAAGATGCTGTACCATCATCACCGCAATACAGTAAGGTAATTGCTGGTGATTACAAACAACTCAAAGATTACGACTTTATCTTTATTGGTGCTGGTCGACCCCAAAAGCAAGGTGGGGAAACCCGGTTACAACTCCTTGAAGGTAATGTCGAAATTATGAAGAACATTGCCAAAGCCGTGAAGGAATCGGGCTTTAAGGGCATTACCTTAATTGCTTCCAACCCAGTCGACATTATGGCTTACACCTATTTAAAGGTAACGGGGTTTGAACCGAATAAGGTAATTGGTTCTGGAACCTTACTAGACAGCGCCCGGTTAAAGTTTGCGATTGCTGAAAAATACGGTATGAGCAGCAGGGATGTCCAAGCATATGTGTTAGGTGAACACGGGGATAGTTCAGTAAGTATTATTTCCTCCGCTAAGATTGCTGGCTTACCACTCAAACACTTCTCGAAAGCAAGTGATATAGAAAAAGAATTTGCTGAAATTGACCACTTTATTCGCCGTCGAGCTTATGAAATCATCGAACGCAAAGGAGCCACCTTTTATGGCATCGGGGAAGCAACTGCCGAAGTAGCAGAATTAATTCTCCGCGACACTAAAGAAGTACGAGTAGTAGCATCGTTAATTAATGGCCAATATGGTGCTAAAGATGTAATGTTTGGCACCCCTTGTGTCTTGGGACGCAATGGTGTGGAAAAGATCTTGGAAATTGAATTGTCTGCTACAGAAAAAGCAGGCTTGGACAAATCAATCCAAGTATTGAAGGACAACATTAAGTTAGCTAAACTATAA", "aa": "MIGGLNQIKMKASLNKLVMKSLKVALIGSGAVGTSFLYAAMSRGLASEYMVIDINEKSQVGNVFDLQDAVPSSPQYSKVIAGDYKQLKDYDFIFIGAGRPQKQGGETRLQLLEGNVEIMKNIAKAVKESGFKGITLIASNPVDIMAYTYLKVTGFEPNKVIGSGTLLDSARLKFAIAEKYGMSSRDVQAYVLGEHGDSSVSIISSAKIAGLPLKHFSKASDIEKEFAEIDHFIRRRAYEIIERKGATFYGIGEATAEVAELILRDTKEVRVVASLINGQYGAKDVMFGTPCVLGRNGVEKILEIELSATEKAGLDKSIQVLKDNIKLAKL", "aa_hexdigest": "853c4494060764869b93776d963abaf6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6490", "uniref100_id": "UniRef100_P78007", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6490", "UniRef:UniRef100_P78007" ] }, "ips": { "uniref100_id": "UniRef100_P78007", "uniref90_id": "UniRef90_P78007" }, "psc": { "uniref90_id": "UniRef90_P78007", "gene": "ldh", "product": "L-lactate dehydrogenase", "ec_ids": [ "1.1.1.27" ], "uniref50_id": "UniRef50_P78007", "cog_id": "COG0039", "cog_category": "C", "kegg_orthology_id": "K00016", "go_ids": [ "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006096", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P78007", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78007" ], "product": "L-lactate dehydrogenase" }, "genes": [ "ldh" ], "id": "CNOCLNGIAL_1406", "locus": "GCA000143945_01406" }, { "type": "cds", "sequence": "contig_1", "start": 793856, "stop": 794161, "strand": "-", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_675", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFE3", "UniRef:UniRef100_P75117", "UniRef:UniRef50_P75076", "UniRef:UniRef90_P75117" ], "nt": "ATGGGCTTTTATGGCAATTTAAACCATATGGAAAAAGGCAAAAGCGGATACGTAACCCATAAACAATTGGACAAAAAACTAGAAGTTTTTAAACAAGAGCTTTTAGTTGAGTTTGATCAAAGATATGTTACTAAGGCAGAATTTAAAGAATTCCAAATAGAAGTTAGAGAAGGCTTTCGAATTCAGGGTGAAGCAATGAACGCCCGCATGGATCGCTTTGAAAGTCTTGTGCTTAAAAGCCTGGAATCAATTAACAATACCTTAATAGACTTTGGCAAACGTATAGACAAATTGGAATCTAAATAA", "aa": "MGFYGNLNHMEKGKSGYVTHKQLDKKLEVFKQELLVEFDQRYVTKAEFKEFQIEVREGFRIQGEAMNARMDRFESLVLKSLESINNTLIDFGKRIDKLESK", "aa_hexdigest": "71ea43af843ad1e29008d9c7697ae0cf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AFE3", "uniref100_id": "UniRef100_P75117", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFE3", "UniRef:UniRef100_P75117" ] }, "ips": { "uniref100_id": "UniRef100_P75117", "uniref90_id": "UniRef90_P75117" }, "psc": { "uniref90_id": "UniRef90_P75117", "product": "UPF0134 protein MPN_675", "uniref50_id": "UniRef50_P75076" }, "pscc": { "uniref50_id": "UniRef50_P75076", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75076" ], "product": "UPF0134 protein MPN_038" }, "genes": [], "id": "CNOCLNGIAL_1407", "locus": "GCA000143945_01407" }, { "type": "cds", "sequence": "contig_1", "start": 794515, "stop": 794652, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003684E99", "UniRef:UniRef100_A0AB38W5S1", "UniRef:UniRef50_A0AB38W5S1", "UniRef:UniRef90_A0AB38W5S1" ], "nt": "ATGGTTGATCGAAGGTTCGCAAACATTGAAGCGGAGTTGAGAGACTTCAAAGTAGAAGTTAGAGAAACTTTTAAAGTTGTGCTTCAGAGCCTTCAAGCCATCAACGAACGTTTAGATAGGCTTGAATACAAGAAATAA", "aa": "MVDRRFANIEAELRDFKVEVRETFKVVLQSLQAINERLDRLEYKK", "aa_hexdigest": "4a5fd6605f621f829c04fd9def02ed38", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003684E99", "uniref100_id": "UniRef100_A0AB38W5S1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003684E99", "UniRef:UniRef100_A0AB38W5S1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W5S1", "uniref90_id": "UniRef90_A0AB38W5S1" }, "psc": { "uniref90_id": "UniRef90_A0AB38W5S1", "uniref50_id": "UniRef50_A0AB38W5S1" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5S1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5497.2922, "isoelectric_point": 8.1404390335083 }, "id": "CNOCLNGIAL_1408", "locus": "GCA000143945_01408" }, { "type": "cds", "sequence": "contig_1", "start": 795428, "stop": 795541, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACAAGCACACTAACCTCAGTGAAATCAGTATGTTGGTAAAGCGTAGTAATGAACTGTTCTTTGAAAATGCACTGTACAAGTGGGCTAACGTTTTCTACCGCCTTTAA", "aa": "MQNKHTNLSEISMLVKRSNELFFENALYKWANVFYRL", "aa_hexdigest": "8b6d8dbb7a728f17c886c7e3bc5f6e96", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4536.196000000001, "isoelectric_point": 9.397636985778806 }, "id": "CNOCLNGIAL_1409", "locus": "GCA000143945_01409" }, { "type": "cds", "sequence": "contig_1", "start": 795575, "stop": 796705, "strand": "-", "frame": 2, "gene": "ydhJ", "product": "putative MG461-like protein", "db_xrefs": [ "COG:COG1078", "COG:R", "GO:0006203", "GO:0008832", "SO:0001217", "UniRef:UniRef50_P47699", "UniRef:UniRef90_P75115" ], "nt": "ATGCAACAAATCTTTTTTAAAGATCCCATTTTAGGGGAAGTGCTGTTTGACCAACAAACTAAGTGGATGTATGAGTTGGTGGAAACGGAAGCGTTTCGCCGTTTACGTAACATTAAGCAGCTAGGAATTAACTTTCATTTTTATCCGGGGGGCGTGCACACGCGTTATTCCCACTCCTTGGGGGTTTATGAACTGTTAAGGCGGATCTTAAACACCCCCGCGTTTGCTCCGATTAATGAAAACAAAAAGCAAACGGTGTTAGTAGCGGGATTACTGCACGACATTGGTCATGCACCGCATTCCCATGCCTTTGAGATTTACTTTGCCAAAGCCCCTAACTTTAAGAAGGAGCTGTTTATCCACGAAGAAGTTACAACCTTATTTGTCAACAGTGAACCGATTAAATCAATCTTAAAGGCGAACCAGATTGATCCTAAACTAGTAGCTGCTTTAATTGATGAAAACAAGGAGTTAAAGCCATCCAACTACTGGATGCGCCAGTTAATTTCATCTGACTTGGATGCTGACCGGATGGATTACCTGTTGCGCGATTCCTACTTTACGGGTACCAGTCATTCCTTGATAGATTACCAAACGATTATTAAGGAGATGGACTGTGTCAAGGTCAAGGGGATTTACGAAATCTTCTTTAAGGATAAGTGCTTGCCCTTAATTGAAAACTTCTTAATTACCCGGCACCACATGTACCAATCAATCTACAGCGATGGCAGGAGTATTTCCACCGAGTTAAACCTTTGGTTTGTCTTTCAACGAATTAAGGACTTAGTGGACAAAAACCAGTTTGACTTCAACGGTTATAAAACCTTAGAACAGGTGTGCTTACCACTACTCAAGAACGAACACTTTGACAAAAAGATGCTGCCAGCGTTTGTTAAGTTAGATGACTATGTGTTCCAATCGTTCTTTGTTAACTTGTACCAAACCACCAAGGACAAGATCTTAAAGAAGTTGTTGGACAGTTATTTAAACAGTTTGAAGTTCGAAATTAAGTTTTACGAAACCAAGGAACAGAGGGACTTAGACTTTGAAAAACAGGCGAGCAAGTACAAGGATGCTAAGTACTTTATTACGAAGTTTAACAATCAGTTTAAGGGCTTTTATGAGGGTTGA", "aa": "MQQIFFKDPILGEVLFDQQTKWMYELVETEAFRRLRNIKQLGINFHFYPGGVHTRYSHSLGVYELLRRILNTPAFAPINENKKQTVLVAGLLHDIGHAPHSHAFEIYFAKAPNFKKELFIHEEVTTLFVNSEPIKSILKANQIDPKLVAALIDENKELKPSNYWMRQLISSDLDADRMDYLLRDSYFTGTSHSLIDYQTIIKEMDCVKVKGIYEIFFKDKCLPLIENFLITRHHMYQSIYSDGRSISTELNLWFVFQRIKDLVDKNQFDFNGYKTLEQVCLPLLKNEHFDKKMLPAFVKLDDYVFQSFFVNLYQTTKDKILKKLLDSYLNSLKFEIKFYETKEQRDLDFEKQASKYKDAKYFITKFNNQFKGFYEG", "aa_hexdigest": "b96947313a171348d4e25d62c23e16ae", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75115", "query_cov": 1.0, "subject_cov": 0.8847058823529412, "identity": 0.997, "score": 759.0, "evalue": 1.08e-276, "valid": true, "gene": "YdhJ", "product": "Uncharacterized protein MG461 homolog", "uniref50_id": "UniRef50_P47699", "cog_id": "COG1078", "cog_category": "R", "go_ids": [ "GO:0008832", "GO:0006203" ] }, "pscc": { "uniref50_id": "UniRef50_P47699", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47699" ], "product": "Uncharacterized protein MG461" }, "genes": [ "ydhJ" ], "id": "CNOCLNGIAL_1410", "locus": "GCA000143945_01410" }, { "type": "cds", "sequence": "contig_1", "start": 796686, "stop": 797147, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "TTGAGTGATAATGCCTACTTTAACTTTATTGATAACTACTTAACGATTGACTTTGATTACCTCAAGGACAAGCGCAAAGAAGTCAGTCTGTTGTTCAAAAACCAACTAGCGTTTGGCATTGAAATTAACCAACTGATTAAGGAAACCTTTGCCCCCAAGTTAGGGGTGCAGCATTTAAGTGTTAAGCACCGTGAACTCTTCAAAGAGCTCCAACCAGCCCTACAACAACTGAGTGAACAACTCCAAGCCCTACCTGATTGGACGAAGGACAATGTCAAAAGTACCTTAACCCAAATTGGTGAACAGTTTAATCTGAAGGGCAAAAAGCTGTTTATGCCACTACGGTTGATCTTTACCAACAAAGAGCATGGTCCTGATTTGGCGGGCATCATGGTGCTGCACGGTAAGACTCAGGTATTAGCGCTGCTTCAAGAATTTATTCATGCAACAAATCTTTTTTAA", "aa": "MSDNAYFNFIDNYLTIDFDYLKDKRKEVSLLFKNQLAFGIEINQLIKETFAPKLGVQHLSVKHRELFKELQPALQQLSEQLQALPDWTKDNVKSTLTQIGEQFNLKGKKLFMPLRLIFTNKEHGPDLAGIMVLHGKTQVLALLQEFIHATNLF", "aa_hexdigest": "2021360d80e92d78197fbf0eabac30b4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 128, "aa_cov": 0.8169934640522876, "hmm_cov": 1.0, "evalue": 5.0877443582785816e-20, "score": 67.79653930664062, "start": 22, "stop": 146 } ], "seq_stats": { "molecular_weight": 17717.375899999988, "isoelectric_point": 7.947871971130372 }, "id": "CNOCLNGIAL_1411", "locus": "GCA000143945_01411" }, { "type": "cds", "sequence": "contig_1", "start": 797283, "stop": 797582, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002849521E" ], "nt": "TTGAAGGCCAATGGCATTGCCACGTATAACTTTGCCGTAGTCATTGATGACCATGACATGGAAATTACCGATGTGTTGCGGGGGGCGGAACACATTTCCAACACCGCTTACCAACTAGCAATTAACCAAGCCTTAGGCTATCAAAAGATTCCCCGCTTTGGTCATTTATCGGTGATAGTCGACAAGAGTGGCAAGAAACTGTCTAAGCGTGATACCAAAACGATCCAGTTCATTGAACAGTTCAAACAAGAGGGTTACCTACCAGAAGCGGTGGTAAACTTCTTGGCCTTACTGGGTTGA", "aa": "MKANGIATYNFAVVIDDHDMEITDVLRGAEHISNTAYQLAINQALGYQKIPRFGHLSVIVDKSGKKLSKRDTKTIQFIEQFKQEGYLPEAVVNFLALLG", "aa_hexdigest": "6f60e53f8aef64bb6786280e22f9b6c7", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0037046112", "query_cov": 0.9292929292929293, "subject_cov": 0.8288288288288288, "identity": 0.598, "score": 114.0, "evalue": 3.63e-31, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002849521E" }, "pscc": { "uniref50_id": "UniRef50_UPI002849521E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002849521E" ], "product": "glutamate--tRNA ligase family protein" }, "genes": [], "id": "CNOCLNGIAL_1412", "locus": "GCA000143945_01412" }, { "type": "cds", "sequence": "contig_1", "start": 797643, "stop": 798140, "strand": "-", "frame": 1, "gene": null, "product": "Glutamate--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "nt": "ATGGAAAAGATTAGAACCCGTTATGCCCCTTCCCCTACCGGTTACCTTCATGTTGGGGGTGCGCGTACTGCTATCTTTAACTTCTTGTTAGCAAAACACTTTAACGGCGAGTTTATTATCCGGATTGAGGACACTGACACTGAGCGCAATGTGGAAGGGGGAATTGAATCACAACTAGAAAACTTGCGCTGGTTAGGAATTATTCCCGATGAATCAATTTACAACCCGGGTAATTATGGGCCTTACATCCAAAGTCAAAAGTTGGCTACCTACAAAAAACTAGCTTATGAACTTGTGGGCAAGGGTTTGGCCTACCGTTGTTTCTGTACTAAAGAAAAGTTAGAACACGAACGCCAATTGGCTTTAGAACACCACCAAACCCCGAAGTATTTGGGAACGTGTCGCAACTTACACTCCAAGCACATTCAAACAAACTTAGACAACCAAGTACCCTTCACGATTCGCTTAAAGATTAACCAGGACGCTGAGTTTGCCTGA", "aa": "MEKIRTRYAPSPTGYLHVGGARTAIFNFLLAKHFNGEFIIRIEDTDTERNVEGGIESQLENLRWLGIIPDESIYNPGNYGPYIQSQKLATYKKLAYELVGKGLAYRCFCTKEKLEHERQLALEHHQTPKYLGTCRNLHSKHIQTNLDNQVPFTIRLKINQDAEFA", "aa_hexdigest": "625e0b899028ac41140e30eaeea8ae4a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A654IDE7", "query_cov": 0.8666666666666667, "subject_cov": 0.9727891156462585, "identity": 0.58, "score": 162.0, "evalue": 2.71e-48, "valid": false, "product": "Glutamate--tRNA ligase", "uniref50_id": "UniRef50_A0A654IDE7" }, "pscc": { "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "product": "Glutamate--tRNA ligase" }, "genes": [], "id": "CNOCLNGIAL_1413", "locus": "GCA000143945_01413" }, { "type": "cds", "sequence": "contig_1", "start": 798127, "stop": 798918, "strand": "-", "frame": 3, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0052908", "RefSeq:WP_010875036.1", "SO:0001217", "UniParc:UPI000012E0AF", "UniRef:UniRef100_P75113", "UniRef:UniRef50_P47701", "UniRef:UniRef90_P75113" ], "nt": "GTGAATAGTTTTTATCCTTCACGCAAATTAGGGCAAAACTTTACGGTTGACCAGAGTGTAATTGCCAAAACCTGTCGGTTAATTAAGAGTCTCAACCCAACGGCCTTAATTGAAGTGGGGCCGGGTAAGGGAGCGTTAACCAAGGCTTTACTCAAGTTACAACTACCATACCATGGCATAGAGTTGGATAAACGCTTAGCGGAATACCTCTTGGTCAACGAAATCTTAACTGAAGAACAACTAACCATTGGTGATGCCTTAAAACAAAACTTGGATCAGTATTTTCCTGACACTATACCTTTGCTTTGTGGCAACATTCCCTACAGTATTAGCTCCCCTTTGATTGCTAACTTTTTGGCTTCCAAACTACAACAATTTGTGCTGGTGTGCCAGTGGGAGTTTGGTCAAAGGTTAGTAGCACCGGTAAATTCGCCTAACTATAGTGCCTTTGGTGTGTTTTGCCAATACCATTTGCAAATTAAATCAGTTTTTAAAATTGATAAGGTTGCTTTTAAGCCCAAGCCCCAAGTGGATTCGGTGTTAATGTTGTTAAAAAAGAAACCCCAAGTAGCCTATGAAGCTCATTTTGGCCGGTTCTTAAAACAGTGTTTTCACCAGCGCCGCAAGCTGTTAGTCAATAACCTCAAGCAGCTTCTCCCACCCACCTTGTTAACTAATGTTTTACAACAACAGGACTTGGCGGCAACGGTTAGAGCCCAAGAGTTAACCCCGACCCAGTTGTTTCGTTTGTATCTTAGCCTTAAACCACATTTAAGCGATGGAAAAGATTAG", "aa": "MNSFYPSRKLGQNFTVDQSVIAKTCRLIKSLNPTALIEVGPGKGALTKALLKLQLPYHGIELDKRLAEYLLVNEILTEEQLTIGDALKQNLDQYFPDTIPLLCGNIPYSISSPLIANFLASKLQQFVLVCQWEFGQRLVAPVNSPNYSAFGVFCQYHLQIKSVFKIDKVAFKPKPQVDSVLMLLKKKPQVAYEAHFGRFLKQCFHQRRKLLVNNLKQLLPPTLLTNVLQQQDLAATVRAQELTPTQLFRLYLSLKPHLSDGKD", "aa_hexdigest": "3bd4dccd0ca3a6ffdda6131a30d13600", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E0AF", "ncbi_nrp_id": "WP_010875036.1", "uniref100_id": "UniRef100_P75113", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E0AF", "RefSeq:WP_010875036.1", "UniRef:UniRef100_P75113" ] }, "ips": { "uniref100_id": "UniRef100_P75113", "uniref90_id": "UniRef90_P75113" }, "psc": { "uniref90_id": "UniRef90_P75113", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_P47701", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0052908" ] }, "pscc": { "uniref50_id": "UniRef50_P47701", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47701" ], "product": "Ribosomal RNA small subunit methyltransferase A" }, "genes": [ "rsmA" ], "id": "CNOCLNGIAL_1414", "locus": "GCA000143945_01414" }, { "type": "cds", "sequence": "contig_1", "start": 798911, "stop": 799210, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATTTATCTCGCAAAAGTTGCCCCAAATTTGGGCTAGCAAACGCAATGAGAATGCTAAAGCGCATTCGCAAAAGAGCATTGAACAGCTCAATAAAACAAAGCGAATGCAGTTAATTTTCTACTTTGTGTTTGCTGTTATTACGGCTTTTAGTGCCGCTGGTGTGGGTGTGTACTGGTTCTTAAATGCCTTGTTTACCTTACTCCAGTCTTATCTAACCCACCTCTTTATTGTTAAAAGGCGTACCAGAAGACGGTTAACGTATTCCAAATTAGAACAAATGTTAGAACGTGAATAG", "aa": "MQFISQKLPQIWASKRNENAKAHSQKSIEQLNKTKRMQLIFYFVFAVITAFSAAGVGVYWFLNALFTLLQSYLTHLFIVKRRTRRRLTYSKLEQMLERE", "aa_hexdigest": "58fb5a875f8968c7bcd6e65d0c32bcd5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11818.760199999988, "isoelectric_point": 10.620344352722167 }, "id": "CNOCLNGIAL_1415", "locus": "GCA000143945_01415" }, { "type": "cds", "sequence": "contig_1", "start": 799244, "stop": 799672, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGCTACCAGGGTTCCTCTAGGTAGCGGTCCGGAATTGGGCTTCAACATGATCTTGTCTTTGATTGTGTTGTTGTTCCTAGTACGGTTAATTACCATAGTGATTACCCTCAACTCCACGCTGGCGTTAGAAAAAATGAACGAGGTCCAGGGCAAATTAGCAGAAATTAATGCCAAGTACAAAGGGGCGTTGGACTTACAGAGCAAACGCAACCGCCAGATGGAGATCATGTCCTTGTACAAGAAGCACAACATTAAGTCTTCTGCTTCCTTTGTTCAGGTCTTTGTTACCTTACCCATCTTCCTCATTATTTACCGGATTGTCACGACTTTAAGACCAATTAAAGCGATTATCCTGTTTAACTTCTGGGACTTATCTAAGGTACCGTTAACGGAAATCTTCTCGAACTTTACCAGTACCGGGTGA", "aa": "MYATRVPLGSGPELGFNMILSLIVLLFLVRLITIVITLNSTLALEKMNEVQGKLAEINAKYKGALDLQSKRNRQMEIMSLYKKHNIKSSASFVQVFVTLPIFLIIYRIVTTLRPIKAIILFNFWDLSKVPLTEIFSNFTSTG", "aa_hexdigest": "2f220ddcb11b846e54f198a5e5a794ec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16096.09469999999, "isoelectric_point": 9.992873573303225 }, "id": "CNOCLNGIAL_1416", "locus": "GCA000143945_01416" }, { "type": "cds", "sequence": "contig_1", "start": 800049, "stop": 800198, "strand": "-", "frame": 1, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "COG:COG0594", "COG:J", "EC:3.1.26.5", "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781", "SO:0001217", "UniParc:UPI0001DD64AE", "UniRef:UniRef100_P75111", "UniRef:UniRef50_P47703", "UniRef:UniRef90_P75111" ], "nt": "ATGCGGGAACAGTTTTGCAATTTAAACGCTGTTGATGTTTTGGTTATTATTAATCAAGGCTTCCTTGAATTAACATTTAAAGAAAAGCAAACGATCTTTTTAAACCTTTGCCAAAAGCTCCAAGAACTCGATGCCCCTAAACCTAAATAA", "aa": "MREQFCNLNAVDVLVIINQGFLELTFKEKQTIFLNLCQKLQELDAPKPK", "aa_hexdigest": "f7b14340e2ac70ad9eba8d9433301b42", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64AE", "uniref100_id": "UniRef100_P75111", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64AE", "UniRef:UniRef100_P75111" ] }, "ips": { "uniref100_id": "UniRef100_P75111", "uniref90_id": "UniRef90_P75111" }, "psc": { "uniref90_id": "UniRef90_P75111", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_P47703", "cog_id": "COG0594", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781" ] }, "pscc": { "uniref50_id": "UniRef50_P47703", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47703" ], "product": "Ribonuclease P protein component" }, "genes": [ "rnpA" ], "id": "CNOCLNGIAL_1417", "locus": "GCA000143945_01417" }, { "type": "cds", "sequence": "contig_1", "start": 800280, "stop": 800405, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCGCTAAGACCAAGCACCACCTCCGTGATCGTAAAGTATTTGCTGCCCTTCTAAGGAGTAAAAGACGCTTCTTCTCGACCTTTTTGATGGCCTACTTTATGCCGAACCGCGTCCGCACTTGA", "aa": "MSAKTKHHLRDRKVFAALLRSKRRFFSTFLMAYFMPNRVRT", "aa_hexdigest": "00ce52d5ef20012e906f3ddf8b70315d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5016.966, "isoelectric_point": 11.999967765808105 }, "id": "CNOCLNGIAL_1418", "locus": "GCA000143945_01418" }, { "type": "cds", "sequence": "contig_1", "start": 800395, "stop": 800541, "strand": "-", "frame": 3, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_010875039.1", "SO:0001217", "UniParc:UPI0000133FBC", "UniRef:UniRef100_P78006", "UniRef:UniRef50_Q7X5L3", "UniRef:UniRef90_P78006" ], "nt": "ATGAAACGTACATATCAACCAAGTAAATTAAAGCGAGCAAAAACCCATGGCTTTTTAGCTCGCATGGCCACTGCTTCTGGTCGTAAAGTATTGAAGTTAAGAAGAAAAAAACAACGCGCCCAACTCACCGTATCCAGTGAGCGCTAA", "aa": "MKRTYQPSKLKRAKTHGFLARMATASGRKVLKLRRKKQRAQLTVSSER", "aa_hexdigest": "077659cf990bc319d68efbe827197fe7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FBC", "ncbi_nrp_id": "WP_010875039.1", "uniref100_id": "UniRef100_P78006", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FBC", "RefSeq:WP_010875039.1", "UniRef:UniRef100_P78006" ] }, "ips": { "uniref100_id": "UniRef100_P78006", "uniref90_id": "UniRef90_P78006" }, "psc": { "uniref90_id": "UniRef90_P78006", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_Q7X5L3", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q7X5L3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7X5L3" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "CNOCLNGIAL_1419", "locus": "GCA000143945_01419" }, { "type": "cds", "sequence": "contig_1", "start": 800564, "stop": 801148, "strand": "-", "frame": 2, "gene": "lolD", "product": "Putative ABC transporter ATP-binding MG467-like protein", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75110", "UniRef:UniRef90_P75110" ], "nt": "GTGATTATTTTAGGTCCATCAGGTTCGGGGAAAACTACCCTTTTAAACCTAATAGGCGGTTATGACTCAATTTCTTTGGGCAGCTGTGTGGTGGCCAATTGTCCCTTGGAAAAATGTACCACCGAACAGCTCTTAACTTACCGCAAGTTAAACCTGGGTTATGTGTACCAACGCTATAACCTCATAGAACTCTTAAGTGCGTATGACAACATTGCCATCTCGCAAAACCTAATTCCTAAAGAACAACGCCACTTAGACATCGAGGAGTTAGCGGCTAAACTCGACATTAAGGAAATCTTGTACAAGTTTCCGTACGAGATGTCGGGGGGTCAAAAGCAACGGGTGGCGATTGCCCGTGCCATTATTAAAGAACCGCGCTTGTTGCTCTGTGACGAACCTACGGGGGCTTTAGACAGTAATTCAGCCGAAAACATTATTGCCTTATTACAAGCAATCAATAAGGAATATAAGCAAACTATTCTAATGGTGACCCACGATGAAACGTTAACGCGGATCGCTAACAGGATCATTAAGATCTCCGATGGTAAGATAGTGTCTAACGAATTGGTGCGTCCTTTATCTTAG", "aa": "MIILGPSGSGKTTLLNLIGGYDSISLGSCVVANCPLEKCTTEQLLTYRKLNLGYVYQRYNLIELLSAYDNIAISQNLIPKEQRHLDIEELAAKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPRLLLCDEPTGALDSNSAENIIALLQAINKEYKQTILMVTHDETLTRIANRIIKISDGKIVSNELVRPLS", "aa_hexdigest": "99686f010059a8b4df434a739a78c711", "start_type": "GTG", "rbs_motif": null, "genes": [ "lolD" ], "truncated": "3-prime", "pseudogene": { "start": 800564, "stop": 801337, "observations": { "mutation": [ 801232, 801313 ], "stop": [ 801232, 801313 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75110", "query_cov": 1.0, "subject_cov": 0.5722713864306784, "identity": 0.995, "score": 377.0, "evalue": 3.59e-130, "gene_start": 1, "gene_end": 194, "reference_start": 146, "reference_end": 339, "reference_sequence": "MKDTEKKTEKELVWDECVDILENVKANSFKATLEYYLASDKKTKKAKPAKVKKVKEPKAKAVKPEQVKPTKTTKAPKPKKPKKQGGLISQWKEKLEAPYRTPYVEQKQGMVISIDKMWKHVHGEDSKEQIAILSDVSLEIGYGEIVIILGPSGSGKTTLLNLIGGYDSISLGSCVVANCPLEKCTTEQLLTYRKLNLGYVYQRYNLIELLSAYDNIAISQNLIPKEQRHLDIEELAAKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPRLLLCDEPTGALDSNSAENIIALLQAINKEYKQTILMVTHDETLTRIANRIIKISDGKIVSNELVRPLS" }, "paralog": false, "identity": 0.9922178988326849, "score": 493.0, "evalue": 3.82e-178, "description": "Internal stop codon at 801232, 801313. Nonsense mutation around 801232, 801313.", "psc": { "uniref90_id": "UniRef90_P75110", "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG467 homolog", "uniref50_id": "UniRef50_P75110", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75110", "UniRef:UniRef50_P75110", "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75110" ], "product": "Putative ABC transporter ATP-binding protein MG467 homolog" } }, "id": "CNOCLNGIAL_1420", "locus": "GCA000143945_01420" }, { "type": "cds", "sequence": "contig_1", "start": 801311, "stop": 801583, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATACAGAAAAGAAAACCGAGAAAGAACTTGTCTGGGACGAGTGTGTTGATATATTAGAAAACGTTAAGGCCAACAGCTTTAAGGCCACCTTTGAGTATTATTTAGCATCAGACAAGAAAACAAAGAAGGCCAAACCCGCTAAGGTGAAAAAAGTTAAGGAACCGAAGGCGAAAGCAGTTAAACCGGAACAAGTTAAACCCACGAAGACCACCAAGGCACCTAAACCCAAAAAACCGAAAAAGCAGGGTGGGTTGATCTCCCAGTGA", "aa": "MKDTEKKTEKELVWDECVDILENVKANSFKATFEYYLASDKKTKKAKPAKVKKVKEPKAKAVKPEQVKPTKTTKAPKPKKPKKQGGLISQ", "aa_hexdigest": "8579796c9bb4ac0319067d9d10f6f670", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10201.946299999992, "isoelectric_point": 9.882503700256347 }, "id": "CNOCLNGIAL_1421", "locus": "GCA000143945_01421" }, { "type": "cds", "sequence": "contig_1", "start": 801624, "stop": 802058, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCCAGCTCTCAAATTAGATGCCGCTGGTGCTAGTGCTCAAATTATCAGGAATAATGCTGAGGTGTTGTTTAATACCGTGAACCAAGTTGATGGGTTCTTGTTGGGCACCATTATTCCCTTTATCTTTATTACCTGTGTGGTCTTGGGGATCTCGATGTTAGAGGAAATGAAACGGATCTTTATTTCCTTAAAATCGATTGGTTACAAAGATAGTCAAAACTTAGTTAGTCTCTTGTGCTTCTTTATTCCCGCTTTTGTACTTTCGTTATTAATCTCGATTGCTATCTTAGCTGGGTTACTAGTAGGAGTACAAGCCCTAGTCTTTGGGGTTGCCCAGGTCTTTTTAACCAACGTGTTTGAATTCTTACCTTACATGGTGGGGATAGTCCTGTTTGGTGCTACCATCTTTGTAATAGGTTCTTACTTCTGA", "aa": "MVPALKLDAAGASAQIIRNNAEVLFNTVNQVDGFLLGTIIPFIFITCVVLGISMLEEMKRIFISLKSIGYKDSQNLVSLLCFFIPAFVLSLLISIAILAGLLVGVQALVFGVAQVFLTNVFEFLPYMVGIVLFGATIFVIGSYF", "aa_hexdigest": "afc3bf22dd50d791de4defe46e49f6af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15601.642999999982, "isoelectric_point": 5.095865821838378 }, "id": "CNOCLNGIAL_1422", "locus": "GCA000143945_01422" }, { "type": "cds", "sequence": "contig_1", "start": 802113, "stop": 803828, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCCCTTGGGTAAATTCCGGGTGTCCTTGATTATTTCCCGGTTATCGCGGTTATTTACCTATGTGGGGTTAAGTTCGATTGCCCTGCTTTTAATTGGGATTGCCGGTACCATCCCGCAAAAGTTTGGGGCAGCGCAGTCCAACACCGTTTTAAACCGCAACTTTAACTATAGGTTGAACTTGCAAACCCCCACAGAACAATCCGGCTGGTATGCAATCCAACCCTACTCCCGGTTTGGTCAAACTGATGATAGTTTGGGGATTAAGGCCTTGTATAAAGATAAGGGTGATCAAATTCAGCAACAGCAACAGCAGCAACAACAGCAAGGCAACGACAAAGAACACCCATACAACCTCAAAGAGTTAAAGATTAGTGATAGGGGCGGAAACCCAATCAAGCATAATGGTAAGGAAATCGAACTCGGTAACCTCCTTCTCCCAAGTTTTGGTGGTGCCCAACAATTAAACACTGACGAAAACTTCTTCCGTCATGCTTCGTTGTCGAAGTGGCTCATTGACTTCCCAATTCGCGTTGGTGGTGCCAACATTAACCCGTGGGAAATTGTCGAGAAGTCCATTCCTAAACAAATTACCCAATTGTTATCGGCTTCCAGTGACCAGTTTTTAATTGCGGTTCTTACTGATGACTACTTTAATAACCTCAACAATAATGGTTTCTTAACCCGCAACCCTCGGACTAACTTTATTCAGTTGGATGCCGCTAGGGTATTAACCCAGATTAACGTCTTCAATCCTGGTGGAGTTAAATTTAACGAACAATTCCTTAAATTCCTTACTAAGGTCTATGGTGATCCGGAGTTGTCCTACCAAGACTCGAAGTTAACGTACGGGATTGTCCCGGTTGATCCACAAATAGAGGAGACATACACCTATGTTCAAGGACCATTTGGTTTTAAGGAAACCGAGTTGAATCCTGATTCCCCTTACACCTTAACGGGAATTAGTCCCGATTCCAAGTTTGTCAACCTCACTGACAGCGGTGGTAACTCCTTGAGGAGCTTAATTAGCTCTGACAGTGAGATGAATGTCATTGTCAACGCTGGCTTTCAGTACGCTAATAACACCAAGATCGGGGACTTCATCTTTATTCAACCCAAGAACACTGCGACCCGCTATTCAGAGAAGTTCTTAAAGAGCCCACCTAAAACACCAACCGTGAAGTTCCGGGTTGTCGGGGTATCTACCGATGCCTTTGGCCAAGAGTTGTATATTAACCAAAACATTGCCAACCGCTTGTTAAAGCTCAATGGCTTTGATGGCCGCGGGGTCATTAAGGATGTGGTGAAGGACGGCCAATCCACCGATGATAGCGGTGGTACTTCGTCTGGCGGTGGCTCTTGTGGGGGTGGAAGTACCTCTTCTACCACCAAAGACAAGTACAAGATTGAGTACGTCAAGCCAACTGGTTATGTTCCCTTTAACGGGGTCTTCTCCAAGGAACTCAACCCTTCTTTGGTAAGTAAAGCGCTAGTACTTAACTCCAACATTGGTGTCTGGGGTAACTTTACGGATTTTGGTAATAACTTTACTAACCTCGTTAAGGGTAAGGAAAACAAGATTATTACCTCAATCTTACCAAGTGATCCGGATATCTTAAAGCAGTTGGCTAAGGAAAAGGGTGAGAATGGTGTGGATAGCATGACCTATGAAAACCTCAGAAAAAAGGTTATCGAAAAATATACGAGCGAATGA", "aa": "MTPLGKFRVSLIISRLSRLFTYVGLSSIALLLIGIAGTIPQKFGAAQSNTVLNRNFNYRLNLQTPTEQSGWYAIQPYSRFGQTDDSLGIKALYKDKGDQIQQQQQQQQQQGNDKEHPYNLKELKISDRGGNPIKHNGKEIELGNLLLPSFGGAQQLNTDENFFRHASLSKWLIDFPIRVGGANINPWEIVEKSIPKQITQLLSASSDQFLIAVLTDDYFNNLNNNGFLTRNPRTNFIQLDAARVLTQINVFNPGGVKFNEQFLKFLTKVYGDPELSYQDSKLTYGIVPVDPQIEETYTYVQGPFGFKETELNPDSPYTLTGISPDSKFVNLTDSGGNSLRSLISSDSEMNVIVNAGFQYANNTKIGDFIFIQPKNTATRYSEKFLKSPPKTPTVKFRVVGVSTDAFGQELYINQNIANRLLKLNGFDGRGVIKDVVKDGQSTDDSGGTSSGGGSCGGGSTSSTTKDKYKIEYVKPTGYVPFNGVFSKELNPSLVSKALVLNSNIGVWGNFTDFGNNFTNLVKGKENKIITSILPSDPDILKQLAKEKGENGVDSMTYENLRKKVIEKYTSE", "aa_hexdigest": "b4487ab33f2c662e1b52f28546df8ee2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 63269.45210000015, "isoelectric_point": 8.779836463928223 }, "id": "CNOCLNGIAL_1423", "locus": "GCA000143945_01423" }, { "type": "cds", "sequence": "contig_1", "start": 803853, "stop": 803978, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAAGACCGAGAACTTGGTTTCATTACCCTGGTCAATCAGGACATTAACCGGTTTACTAAAGAGCTGTTTAAAGGCTATTCAGCTAATAAACTGGAAGATCACAAAGATCAACAGCATTGA", "aa": "MQEDRELGFITLVNQDINRFTKELFKGYSANKLEDHKDQQH", "aa_hexdigest": "d53d7337194de875612b233604a11f18", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4937.417500000001, "isoelectric_point": 5.473106193542479 }, "id": "CNOCLNGIAL_1424", "locus": "GCA000143945_01424" }, { "type": "cds", "sequence": "contig_1", "start": 803937, "stop": 804587, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "TTGTCCACAAACTCACCGCTGCCCAACTCCCAGGATGAAGGCTTAATCTTTCTCAATGAGCAGGGATACCGCAGTGTCTTGTTTGCTGTGCCTGCTGCTAGTGAGGAGAACTACTACGCCTTTAAGTCGGATGACATTAAAGCGAAGTTCCCTGGCCAAGATCCGATCCAGGTGGTGGCCTCTAAGTTAAAGGGCTATCTCAACGTCCCTGACAGTGATCTCGCCTTTAACGTTAAGGACATCTCCAAGTTCCAGTACTTAACTACAGCCCGGAACTATTTCCCGGACTTAGTGCAGAACTATTTAGCCATTGCCAGTGTGGTCATTGCGGCCTTCTTATCCATCTTGGCCTTATACCTCACCATTCTCTTAATTAAGTCTTTTATTAAGAAGAACCAAACGGAGTTCTCAATTATCCGCGCTGGTGGGTTCTCCACAGCCAAGTTTATTGCCGGCATGAGTGTGTTTGCCGGCATAGTAGCCTTGGCCTCATCCTTCTTTGGGGTCTTATTTGCCTTCCTTTTAGAAAGACAAGTTAAGGGGATTATCTCCAGGTATTGGTTTATAGCCCTACCGGCCAACAGCTTTAACTGGATTTCCTTCTTTGGATCAATGCTGTTGATCTTTGTGATCTTCCAGTTTATTAGCTGA", "aa": "MSTNSPLPNSQDEGLIFLNEQGYRSVLFAVPAASEENYYAFKSDDIKAKFPGQDPIQVVASKLKGYLNVPDSDLAFNVKDISKFQYLTTARNYFPDLVQNYLAIASVVIAAFLSILALYLTILLIKSFIKKNQTEFSIIRAGGFSTAKFIAGMSVFAGIVALASSFFGVLFAFLLERQVKGIISRYWFIALPANSFNWISFFGSMLLIFVIFQFIS", "aa_hexdigest": "658120ad7211e625058e72ed5c370a57", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 109, "aa_cov": 0.5046296296296297, "hmm_cov": 0.981651376146789, "evalue": 5.5391299853290605e-12, "score": 41.29035568237305, "start": 108, "stop": 216 } ], "seq_stats": { "molecular_weight": 24064.715899999992, "isoelectric_point": 9.023333549499512 }, "id": "CNOCLNGIAL_1425", "locus": "GCA000143945_01425" }, { "type": "cds", "sequence": "contig_1", "start": 804723, "stop": 805568, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGAAGGTCACCGCGTTATTAGAAAAATACCGCGATGCCTTCTTAAAGGCGAAAGCCTTAAAGGAATTGGAGTTTTCCCGGCAAAACTTAGCGATTGCTACCAATGTTAGCTCCGAAGAATCCGCTTCCTTCTTGGTCTCCAATAAGGATTCCCAGAAGTACAACGACCTTTCTATTATTGAAGGGATTGACTTTAAGTCTTGGCTTGCCAAGGAAAAAAGCAATCCTTTAGACATGGTATATGGGGGCAAGAGTAACAGTGAAGGCTTCTTGGAAAAGGTTGAGTATGAGTTTAAGCCTACTCAAACAGATGAAAAGAAAAAAGCAGCAGCCAAAACTACGCAAGGCACAACGGACTCTTTAACCCAGTTAGCAGATGCATCTTCTTCCTCCAGCTCCTCTTCAACTGGTGATACTAAGAGTACTTCTACCAAATTCCAAATCTACCCCAAACTAGCTAACATCTTAGCCCAAGCCCAACTCCCCGAGGCTTCCAGTATTCCTGATACCTTAACCAATGCCATTAAGCAGTGGTCTACATTAGATAAAAAAGGCTTTGAGGCTTTAGATGATACAGGTAAGAGTAAAGCCGCTAACAACTATGTTGCTTTATTGAGTTACTTTACCCCGGAGTTTCAAGATCCGAATGAACTGGTAGTAACTAACCGCCAAAAGTTAGATATCCCGATTATCTTTAAAAACGGGGTTAACCCGTTAACACTACCCACCGACCAACAGTCCTTGGTAGTCCAAACCCCGGAAGCCCACGGTGCGGTGGTATCCCAGCAATGGTTATTTAAACACGACAAGGAAGTATTACCTTTAGAAGGGGAATACTCCTGA", "aa": "MKKVTALLEKYRDAFLKAKALKELEFSRQNLAIATNVSSEESASFLVSNKDSQKYNDLSIIEGIDFKSWLAKEKSNPLDMVYGGKSNSEGFLEKVEYEFKPTQTDEKKKAAAKTTQGTTDSLTQLADASSSSSSSSTGDTKSTSTKFQIYPKLANILAQAQLPEASSIPDTLTNAIKQWSTLDKKGFEALDDTGKSKAANNYVALLSYFTPEFQDPNELVVTNRQKLDIPIIFKNGVNPLTLPTDQQSLVVQTPEAHGAVVSQQWLFKHDKEVLPLEGEYS", "aa_hexdigest": "f56050dcc3cad352fdd52eb05347d396", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31021.418700000017, "isoelectric_point": 5.449574851989746 }, "id": "CNOCLNGIAL_1426", "locus": "GCA000143945_01426" }, { "type": "cds", "sequence": "contig_1", "start": 805593, "stop": 805814, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGCACTTCGCTCTTCTGTTTCTTTCAAGCCGCAATTACAACAAACGAACGTTGCGTTGGCTCAGCAACAACAAGATAAGCAGGAAAGTAGTGCAGATGACGGTGTAAAAGATCCAACTTTTAGTGATGTCCAAACTGAATTCGACAAAATTGGCACCGAAAACCACACTCCGCAAAAGAATTTAAATAACGTTTATGCTGCCCTTTTACACCAATGA", "aa": "MIALRSSVSFKPQLQQTNVALAQQQQDKQESSADDGVKDPTFSDVQTEFDKIGTENHTPQKNLNNVYAALLHQ", "aa_hexdigest": "056c214fb13dbbed568e977ab32818e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8143.847, "isoelectric_point": 5.034650230407714 }, "id": "CNOCLNGIAL_1427", "locus": "GCA000143945_01427" }, { "type": "cds", "sequence": "contig_1", "start": 806061, "stop": 807233, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCGTTTTTTAAACAGATCTTTAAGTCACTCAAAAAGTTCTTCTTTTTACTTTTTGGGATTATTTTCGTCCTTTTCAGCATTATCTTCCTGGAAACGTCGATTCTTCAGTTAAGTAATAACCTTGTTAATACTTATACTGCCCTTGTTCAAAAAACGAATTCTTCTGACATAGTAGCACCGGCTATCTTTAAAGAAAGTAGTCCGGTGTATAAAACGGAACTTAAAGAAGAAAAAAGACATTTTTCCAAAATCAAGTTAACCGAAAAGAAGATTAACTTTATCTGGCCCTACCAAGAATCTGATTTTGGGAGTGATTCTGAAAAGAAAGATTCAACCACCAAATCATCAGATAATAACCCACGAAAAGGTGATGTTAACGATAAAGACAAACTCTTTTTAGCTCGTAAAAGAGGCATTTTGAAAGCTTACGGGGAAGCCAACATTGCCGAAAAACGGATCTATAAGGGTTTAGCTGTTTCCTTTCAAAATACCTATTCTTTTACTGGAACCGATCAAGAAAGTAATAACTTAAACCAAAATACGGTTTCCGATCCCCAAAACCTGATTTATGACAAAGAAGGTAACCTTTTAGGTTACTTTGTCGATGGTTTAATTCTGGATGGGATTCCCCTGCGCGCTGGGATTGCTCGCTTTCCTGGTGATAAAGGGAAGGGTGAGGATAAGAAAACTACTAAAAAAGAATCAGAAATCAAGCAAGCGAGTAGTGCCACAACAGTTTTACAACCGTTAGCAGCTCAGGCCAAGATGACTGATGCTAAAGAAACCACAAATAACGAGGAGCCCAAAAAAGATAGTAATGTAGAAGAACAATACACTACCAATAATAAAGACAAAGTTTGGTTTAAAAGTGATGAAACTCAAGCTGGTAGTAGCAGTGGAGAAAGCGAAACCAGTAAACTAAGTACCTCTTATCTATTTACCGGAGGGCAAGAAGCTGCTAACTGGTTTCCCAATCTGTACGCTAATGTACCTATTGTGCTCCCGATTAGTCCGGGTTCCCAGTTTTGGTTAGAGACTAATCCCTTTAAAGAAATAATAGAGGTCTTCCAAAAAGAAAAAGAGGAAAAAGAAAAACAATCTTTCTCCTTAACTTTTACTTTAGATACAAGTAAGTTATCTCATTTAGATAAGGAAGAATTTGACTGA", "aa": "MFSFFKQIFKSLKKFFFLLFGIIFVLFSIIFLETSILQLSNNLVNTYTALVQKTNSSDIVAPAIFKESSPVYKTELKEEKRHFSKIKLTEKKINFIWPYQESDFGSDSEKKDSTTKSSDNNPRKGDVNDKDKLFLARKRGILKAYGEANIAEKRIYKGLAVSFQNTYSFTGTDQESNNLNQNTVSDPQNLIYDKEGNLLGYFVDGLILDGIPLRAGIARFPGDKGKGEDKKTTKKESEIKQASSATTVLQPLAAQAKMTDAKETTNNEEPKKDSNVEEQYTTNNKDKVWFKSDETQAGSSSGESETSKLSTSYLFTGGQEAANWFPNLYANVPIVLPISPGSQFWLETNPFKEIIEVFQKEKEEKEKQSFSLTFTLDTSKLSHLDKEEFD", "aa_hexdigest": "0f29f60cd8cf78a70cbef571f826d49b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 44162.08070000007, "isoelectric_point": 6.015975379943847 }, "id": "CNOCLNGIAL_1428", "locus": "GCA000143945_01428" }, { "type": "cds", "sequence": "contig_1", "start": 807245, "stop": 807910, "strand": "-", "frame": 2, "gene": "lolD", "product": "Putative ABC transporter ATP-binding MG468-1-like protein", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_Q50316", "UniRef:UniRef90_Q50316" ], "nt": "GTGACCAATGGGATCACAAACCAGTTAATCTGTGATCACATTGATCTCAAAATTGCCCCTGGGGAGTTTGTGGTTATTTTGGGTAAATCTGGTTCTGGTAAGACTAGTTTACTCTCTTTAATCTCCGCCCTAGACCGACCTACTAGCGGGGTGAGTTTTGTGTGTGGTAGGAGTACTATTTGTTGTAATGATGCCCAATTAACATCACTACGGAATAAAAACGTAGGTTATATATTCCAACAGTATGGCCTTTTAAGGGATCTGAACGTTGATGACAACATTAAACTGGCAGTTCCCTTTAAAAAGCGCCATAACAACAATTTGGAAGAGCTGTTAGAGCGGTTGGAGCTCAAAGAGCACCGTAATAAAAAGATTACCAAGCTCTCTGGGGGACAACAACAACGGGTAGCAATTGCGCGGGCACTAATCAAAGAGCCGCGTATTTTATTTGGTGATGAGCCAACTGGTGCTGTTAACGTGGATATTTCCAAAAAGATCCTCCAGTTCTTTGTGGAGTATAACCGCGATAAGGGCACTACTATAGTATTAGTTACCCACAATGAAAAGATTGTGGAGCTAGCTAAACGGGTCATTAAGATCCACGATGGCAAGATTGTGGCTGATTATCTTAACCAAAGACCCAAGACTATTAACGAAATTAACTGA", "aa": "MTNGITNQLICDHIDLKIAPGEFVVILGKSGSGKTSLLSLISALDRPTSGVSFVCGRSTICCNDAQLTSLRNKNVGYIFQQYGLLRDLNVDDNIKLAVPFKKRHNNNLEELLERLELKEHRNKKITKLSGGQQQRVAIARALIKEPRILFGDEPTGAVNVDISKKILQFFVEYNRDKGTTIVLVTHNEKIVELAKRVIKIHDGKIVADYLNQRPKTINEIN", "aa_hexdigest": "9b2835fec29bde0f45f83c1213c1044d", "start_type": "GTG", "rbs_motif": null, "genes": [ "lolD" ], "truncated": "3-prime", "pseudogene": { "start": 807245, "stop": 808093, "observations": { "mutation": [ 808045 ], "stop": [ 808045 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50316", "query_cov": 1.0, "subject_cov": 0.775438596491228, "identity": 0.995, "score": 428.0, "evalue": 1.3e-150, "gene_start": 1, "gene_end": 221, "reference_start": 63, "reference_end": 283, "reference_sequence": "MSLNAKNKRSLDYCLQWPDFCQSKKASKLIVKLNKKHPKRRXHYKDPEAKHYDVLFKGVCKAVTNGITNQLICDHIDLKIAPGEFVVILGKSGSGKTSLLSLISALDRPTSGVSFVCGRSTICCNDAQLTSLRNKNVGYIFQQYGLLRDLNVDDNIKLAVPFKKRHNNNLEELLERLELKEHRNKKITKLSGGQQQRVAIARALIKEPRILFGDEPTGAVNVDISKKILQFFVEYNRDKGTTIVLVTHNEKIVELAKRVIKIHDGKIVADYLNQRPKTINEINWV" }, "paralog": false, "identity": 0.9929328621908127, "score": 555.0, "evalue": 4.74e-203, "description": "Internal stop codon at 808045. Nonsense mutation around 808045.", "psc": { "uniref90_id": "UniRef90_Q50316", "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG468.1 homolog", "uniref50_id": "UniRef50_Q50316", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50316", "UniRef:UniRef50_Q50316", "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q50316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50316" ], "product": "Putative ABC transporter ATP-binding protein MG468.1 homolog" } }, "id": "CNOCLNGIAL_1429", "locus": "GCA000143945_01429" }, { "type": "cds", "sequence": "contig_1", "start": 808162, "stop": 809481, "strand": "-", "frame": 3, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "GO:0003688", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006270", "GO:0006275", "GO:0016887", "KEGG:K02313", "RefSeq:WP_014325680.1", "SO:0001217", "UniParc:UPI0001DD64B1", "UniRef:UniRef100_A0AAV5NA35", "UniRef:UniRef50_P35888", "UniRef:UniRef90_Q59549" ], "nt": "ATGGAACAATTTAGTGCTTTTAAACTTCTTTTAAAAAAGCAATACGAAACTACTTTAGGTTTTTATGACAAGTACATTAAGAATCTTAAACGGTTTGCTTTAAAAAATAATGTCCTTTTTGTGATTGTGGACAATGAGTTTGCCCGTGAAACCCTCAATAGCAACCCTGATATTGTCGGGTTAGCTAGCAAACTGTACGATGACATTAGAGCAGTTCGGTTTGTCAACGAACAGGACTTCTTTATCAATTTAGCCAAACTAGAGGAAGATAACCGCGAAACGCTTTACCAAAGTTCGGGGCTAAGCAAAAACTTTACCTTTAAAAACTTTGTGGTTGGTGATGGCAACCGCCGGGTTTATGAAGCGGGTGTACGGATTAGCGAAACTCAGGACGCTGATTTCTCCCCCTTATTTATCTATGGGGAGACTGGTCTGGGTAAAACCCACTTACTCCAAGCGATTGGGAATGATAAGTTCTTCCACTTTCCCAACGCTAAGGTAAAGTATGTGGTATCGAGTGACTTTGCCCAGGAGGTTGTCAATGCCTTTTACCAAAAGGACAAGGATCAAGGCATTGAAAAGCTCAAGGCTGCTTACGAGTCATTGGATTTACTCTTAATTGACGATACTCAGATCTTCGGGAAAAAGGAAAAAACCTTAGAGATCCTCTTTTCCATCTTTAATAACTTAGTAAGTAAGGGCAAGCAAATTGTGTTTGTGTCCGATAAAACCCCCGATGAGTTGGCCAACATCGATCCACGGATGGTATCACGCTTTAAATCGGGACTCTTGTTAAAGATTGAAAAACACAATTTGAGCTCCCTGTGTGAAATTTTGGCCGTTAAGCTCAAAGAAAAGGATCCCAACATCCAAATTACCAATGAAGCACGCGAAAAGGCAGCGCAAATATCTGGTAACGATGTTCGTTCTTTAAACGGGATTGCGACTAAGTTGCTGTTTTATGTCAAAACAACTAAGCAAAACCTGATTAACAACGACAATCTAAAGGAAATCCTGTTTGAGGAATTTGAGAAGTTCCACAAAAAGAGCTTTGATCCTTATTTATTAATAGAAAACGTTTGCCGCCGCTTTAATGTACCGGTGGACAGTGTTTTGTCTGAGAACCGTAAAGCAGAGTTAGTTCGTGTTCGTGACGTTTGTAACTATCTTTTAAGGGAAAAGTACAAGATGCAGTTCCAACAAATAGGCAAAATTTTTAAAAGGAACCACTCTACAGTGGTGGCTGCAGTTAAAAGGGTTGCTAAAATGATTGAGAAGGACGACTCCCTCCAAGACATTATCACTTCCTTGGTAATTTAG", "aa": "MEQFSAFKLLLKKQYETTLGFYDKYIKNLKRFALKNNVLFVIVDNEFARETLNSNPDIVGLASKLYDDIRAVRFVNEQDFFINLAKLEEDNRETLYQSSGLSKNFTFKNFVVGDGNRRVYEAGVRISETQDADFSPLFIYGETGLGKTHLLQAIGNDKFFHFPNAKVKYVVSSDFAQEVVNAFYQKDKDQGIEKLKAAYESLDLLLIDDTQIFGKKEKTLEILFSIFNNLVSKGKQIVFVSDKTPDELANIDPRMVSRFKSGLLLKIEKHNLSSLCEILAVKLKEKDPNIQITNEAREKAAQISGNDVRSLNGIATKLLFYVKTTKQNLINNDNLKEILFEEFEKFHKKSFDPYLLIENVCRRFNVPVDSVLSENRKAELVRVRDVCNYLLREKYKMQFQQIGKIFKRNHSTVVAAVKRVAKMIEKDDSLQDIITSLVI", "aa_hexdigest": "add7551f8fa14efb87eb7b45cf078d1f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64B1", "ncbi_nrp_id": "WP_014325680.1", "uniref100_id": "UniRef100_A0AAV5NA35", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64B1", "RefSeq:WP_014325680.1", "UniRef:UniRef100_A0AAV5NA35" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NA35", "uniref90_id": "UniRef90_Q59549" }, "psc": { "uniref90_id": "UniRef90_Q59549", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_P35888", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "go_ids": [ "GO:0005737", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0003688", "GO:0006270", "GO:0006275" ] }, "pscc": { "uniref50_id": "UniRef50_P35888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35888" ], "product": "Chromosomal replication initiator protein DnaA" }, "genes": [ "dnaA" ], "id": "CNOCLNGIAL_1430", "locus": "GCA000143945_01430" }, { "type": "cds", "sequence": "contig_1", "start": 809481, "stop": 810233, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_687", "db_xrefs": [ "RefSeq:WP_010875044.1", "SO:0001217", "UniParc:UPI000013AFF4", "UniRef:UniRef100_Q50315", "UniRef:UniRef50_Q50315", "UniRef:UniRef90_Q50315" ], "nt": "ATGGCCATCAGTAAAAAGAAACGCTTCTTCTTTGACCTAGCTCAGGATGAAGATGATGCAGAAACTGTTCAAGAAGTTAAAAAGGTAGAACAACAATTAAAACTAGAACCGGTTGTCCAACCCCAACATGATCTAACTAACCAAACTAAAGCAAATCAATCTAGTCAAGACAGAAAGTTCTTTTCTAAGGACATGCCCCAGTTTGATTTTGGACCATTACTCAAGTTTGGCGATGAGTTTGTGAAATCCTTTAACCAGTTTCCCAAACAAGAACCACAAACTTCAACCCAACCAGTTAATGTGCAACCGCAATCAGAACCAACTAACTTTAATAATCAGGTTCCAACCCAACCAGTTCACCAAACTGCAGAAGTGCACTTAAATGAGTTTCAACAACCTACAACCACTAACTTTAATCAACAACCAGTAGCTACATCTAATATTCAGGTGGAGGCTACGCAACCAATTGTGGAACCTGTTCCCCAACCAGAACCACAACCAGCGGTTGAACAGCCACAAGTTAAGCAGACTACTCGCCCATCTAATAAATTGCAGGAAGAAGAAAATCTACCACCACCAAAGGCGAAAGTGCCCGGTATTATTCCCTTAGAACGGCAAGAACGCTTAACCACCGGGGTGCACTTTTATACGAGTACCCGTGTTTGGAATAAGGTTAAGCGTTATGCTAAAGCGGTTAACATTCCGATTTCGCGGATCTTAACGATGATTCTTGATCAGGTAATAGAAGAGTAA", "aa": "MAISKKKRFFFDLAQDEDDAETVQEVKKVEQQLKLEPVVQPQHDLTNQTKANQSSQDRKFFSKDMPQFDFGPLLKFGDEFVKSFNQFPKQEPQTSTQPVNVQPQSEPTNFNNQVPTQPVHQTAEVHLNEFQQPTTTNFNQQPVATSNIQVEATQPIVEPVPQPEPQPAVEQPQVKQTTRPSNKLQEEENLPPPKAKVPGIIPLERQERLTTGVHFYTSTRVWNKVKRYAKAVNIPISRILTMILDQVIEE", "aa_hexdigest": "1fa6f86f9aea8ab7acbbe64c6fd4afe0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AFF4", "ncbi_nrp_id": "WP_010875044.1", "uniref100_id": "UniRef100_Q50315", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFF4", "RefSeq:WP_010875044.1", "UniRef:UniRef100_Q50315" ] }, "ips": { "uniref100_id": "UniRef100_Q50315", "uniref90_id": "UniRef90_Q50315" }, "psc": { "uniref90_id": "UniRef90_Q50315", "product": "Uncharacterized protein MPN_687", "uniref50_id": "UniRef50_Q50315" }, "pscc": { "uniref50_id": "UniRef50_Q50315", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50315" ], "product": "Uncharacterized protein MPN_687" }, "genes": [], "id": "CNOCLNGIAL_1431", "locus": "GCA000143945_01431" }, { "type": "cds", "sequence": "contig_1", "start": 810220, "stop": 811032, "strand": "-", "frame": 3, "gene": "parA", "product": "ParA family protein MPN_688", "db_xrefs": [ "COG:COG1192", "COG:DN", "KEGG:K03496", "RefSeq:WP_010875045.1", "SO:0001217", "UniParc:UPI0000131320", "UniRef:UniRef100_Q50314", "UniRef:UniRef50_P47706", "UniRef:UniRef90_Q50314" ], "nt": "ATGATTATTTCTTTTGTTAATAACAAAGGTGGCGTTTTAAAAACAACCATGGCCACTAACGTTGCTGGTTCGCTTGTAAAGTTGTGCCCTGAACAACGGAAAGTTATTTTAGACCTTGATGGTCAGGGCAACGTTTCAGCCTCTTTTGGACAAAATCCAGAAAGATTAAATAACACCTTAATTGATATTTTGTTAAAAGTGCCTAAGTTTAACGGCGCCAACAGTTCAATTGAAATTGATGACTGTTTGTTGCCCGTTTATGAAGGACTAGACATTTTGCCTTGTAACTTTGAGTTAAATTTTGCGGACATTGATATTGCTCGCAAAAAGTACAAGGCTTCTGATATTGCGGAAATAGTTAAACAACTCACCCGCAGGTATGACTTTGTTTTACTAGACACACCGCCTAACATGGCTACCTTGGTCTCCACAGCCATGAGCCTCTCTGATGTGATTGTGATCCCGTTTGAACCTGATCAATACTCTATGTTGGGTTTAATGCGGATTGTCGAAACGATTGATACTTTTAAGGAAAAGAACCCCAACTTAAAAACCATTTTGGTACCAACCAAGGTCAATATGCGCACCAGATTACACAATGATGTGATTGAGTTGGTGAAATCAAAAGCGCACAAGAACAACGTTGCTTTTTCAGAACACTTTGTATCTTTAACATCTAAGTCATCTGCTGCTGTTGGTTATGAAAAGTTACCTATCTCTTTGGTATCACCAACATCCAACAAGAAGTACCAAACTGAATATTTAGAGATTACTAAAGAAATTCTTAACCTAGTCAACCATGGCCATCAGTAA", "aa": "MIISFVNNKGGVLKTTMATNVAGSLVKLCPEQRKVILDLDGQGNVSASFGQNPERLNNTLIDILLKVPKFNGANSSIEIDDCLLPVYEGLDILPCNFELNFADIDIARKKYKASDIAEIVKQLTRRYDFVLLDTPPNMATLVSTAMSLSDVIVIPFEPDQYSMLGLMRIVETIDTFKEKNPNLKTILVPTKVNMRTRLHNDVIELVKSKAHKNNVAFSEHFVSLTSKSSAAVGYEKLPISLVSPTSNKKYQTEYLEITKEILNLVNHGHQ", "aa_hexdigest": "2a80685578b86c1abf65fb4d027b6b5e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000131320", "ncbi_nrp_id": "WP_010875045.1", "uniref100_id": "UniRef100_Q50314", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000131320", "RefSeq:WP_010875045.1", "UniRef:UniRef100_Q50314" ] }, "ips": { "uniref100_id": "UniRef100_Q50314", "uniref90_id": "UniRef90_Q50314" }, "psc": { "uniref90_id": "UniRef90_Q50314", "gene": "ParA", "product": "ParA family protein MPN_688", "uniref50_id": "UniRef50_P47706", "cog_id": "COG1192", "cog_category": "DN", "kegg_orthology_id": "K03496" }, "pscc": { "uniref50_id": "UniRef50_P47706", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47706" ], "product": "ParA family protein MG470" }, "genes": [ "parA" ], "id": "CNOCLNGIAL_1432", "locus": "GCA000143945_01432" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Mycoplasmoides pneumoniae] [strain=FH] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TATTTACCGACGAAATTAATACCATCAGGGTATTAAGATGCTACCAACGTGGTATTAAAATGTGCCCAACCGCGAAAAAGAAAGTGGTATATAGGAAAATGGAAAACAAATTTGTTTACCGTTTACTATTAAATACTAATCTTCTATATAGTATAGAGAAACTTTTTCTTTAACATAATATTATCTTAATATTATTTACCTACTAATAGCTTAATATTATTAGTATTTATTTAGTATTATGCTAATACTATGCAGATATTATCTTAATATTATCTATAGTATTAGGCTAATATTATTCTTAATATTTATTAAGGTACTAAAGCATTACCTATAGGTGATATTATGACAATACTAAAGTGGTTAGTATTATTAGGGTATTATTCAAAGTATTCTCCAACACTATTCCCTTAGCTCTAACTTAATATCCCTTTAATACCAAGATGGTTAAACAATGAAGAAAATTAGTTGTTTGTTCTGGCCCTTTACTATATATGTACATAGTACATATATAAGAAAGGCTACCATGACATTATATATAATAGGCATCCAGAAATAAAAGCCAAATTAATCTATAATTAAGTATTTATACTTAATTATAGTGATTTTATTAACAAAATTAGTGACATTTGGCCATCCATAATAAATAAAATCAAATAAACAAAACAAAAAGAAAAAAACTATAAAAAGGGCATGAAAGTTTTGATTAATAAGAATGAGTTGAACAAAATCCTCAAAAAACTCAACAATGTAATCGTATCTAACAATAAGATGAAACCATACCACTCTTATTTATTAATAGAGGCTACAGAAAAGGAAATTAACTTCTATGCTAACAACGAGTACTTTTCTGCTAAATGTACCTTAGCCGAAAACATTGATGTACTTGAAGAAGGTGAAGTAATTGTTAAAGGCAAAATCTTTAGCGAACTCATTAACGGAATTAAAGAAGACATCATTATTATTCAAGAGAAAGATCAAACTCTTTTAGTCAAAACAAAAAAAACAAACATTAACCTTAACACGATTGATAAGAAAGAATTCCCCAGAATCCGTTTCAACCAAAACGTTGATTTGAAGGAATTTGATGAACTTAAAATCCAACACAGCCTTTTAACTAAAGGACTTAAAAAGATTGCCCATGCTGTTTCTACATTTAGAGAATCCACTAGAAAATTCAACGGGGTTAACTTCAACGGTTCCAATGGTAAACAAATCTTTTTAGAGGCATCGGATTCTTATAAGCTCTCTGTTTATGAAATCAAACAAAAAACCGATCCATTTAATTTCATTGTCGAAACTAATCTTTTGAGCTTCATCAATTCTTTTAACCCTGAAGGTGGTGATTTAATCAGTATCTTCTTCCGCAAAGAACACAAGGATGATTTAAGTACCGAATTACTGATTAAGTTAGATAACTTCTTAATTAACTACACCTCAATTAACGAAAGCTTTCCGCGGGTAATGCAGTTGTTTGACTTTGAACCAGAAACCAAAGTAACCATTCAAAAAAACGAACTTAAAGATGCATTACAAAGAATCTTGACGTTAGCTCAAAACGAGCGCTTCTTTCTCTGTGACATGCAAGTAACCAACTCCCACCTCAAAATTAATTCCAACGTTCAAAACATTGGTGCATCCTTAGAAGAAGTTACTTGCCTTAAGTTCGAAGGTCACAAACTCAACATCGCTGTTAATGCGCTTTCCCTCTTGGAACACATTGACTCATTTGATACTGATGAAATTGAGCTTTACTTCCAGGGCAGTAACAAATACTTTTTAATTAGTTCGAACAACGAACCTGAACTTAAAGAAATCCTAGTTCCTTCCAAGTAACTAATGACGCTTTATGACTTACTAGAGCTGCCCCAAACTGCTACACTACAGGAAATCAAAACAGCTTACAAACGCTTAGCTAAACGTTATCACCCTGACATCAATAAACAAGGGGCAGATACCTTTGTCAAGATTAATAACGCCTATGCCGTTTTAAGCGATACCACCCAAAAAGCGGAGTATGACGCTATGCTACGCTTTAGTGAATTTGAAGACCGAGTTAAACGCCTGGACTTCTCGATTAAGTGACACGAACAGTTTATGGAGGAATTACAGTTCCACCACAACTGGGACTTTGACTTTATCCGCAACAGGGAATACACCCAGCCAACCCCTACCAACAATAAGTATTCTTCCTTTTTAGACAAGGATGTATCCTTGGCCTTTTACCAACTTTACTCCAAGGGCAAGTTGGACTTTGACTTGGAAGACACACTTCTTAGACGTCATTCGATCAAGCAAGCCTTCTTAAAGGGCAAGAAGCTTAACGATGTTTTAAAAGAGCAGTACAACTATTTAGGTTGATTAGAGGCCAAGCGCTATTTCAACATTGATGTCGAAATCGAATTAACCCCCAAAGAAGTGCGTGAAGGTGGGGTGGTCAACCTCCCTTTAAAGATCAAAGTCATTTCCAATAACTACCCTGGACAAATGTGGTACGAACTCAACAAGAACTATTCCTTCCGGCTTCTTTGAGACATTAAAAACGGTGAGGTGGCAGAGTTCTTTGGCAAGGGTAATCGGGCCTTGGGTTGACGCGGTGATCTGATTGTGCGAATGCGGATTGTCGACAAAATTAAGAAGCGCTTACGCATCTTTTCCAGTCATTTTGAACAGGACAAAACCAAGTTGTGATTCCTCGTGCCGCAGGACAAACAAGACAATCCTAATAAGTGGGTTTTTGACTATAAAACACACGAGTTTATTGTTTAAAATCGCTTAATTTTTAATGTGGTATAATTGTTTGGATTCGCCATAAATTTTGCTTATGGCTTTTAACTTTCCAACGTGCACATTAAATGGAAGACAATAACAAAACGCAAGCTTACGATTCCAGTAGCATTAAGATTCTTGAAGGCTTAGAGGCCGTGCGTAAGCGCCCCGGCATGTACATCGGTTCCACCGGCGAAGAAGGGTTGCACCACATGATCTGGGAAATCATTGATAACTCAATTGACGAGGCCATGGGTGGCTTTGCGAGTACTGTCAAACTAACCCTTAAAGACAACTTTGTCACCATTGTGGAGGATGATGGCCGGGGCATTCCCGTTGACATCCATCCTAAGACCAACCGCTCCACCGTTGAAACAGTGTTTACGGTACTCCACGCTGGGGGTAAGTTTGACAACGATAGCTATAAGGTGTCAGGGGGACTCCACGGTGTGGGTGCCTCGGTTGTTAATGCTTTAAGTTCCTCCTTTAAGGTATGGGTAGCCAGGGAGCACCAACAGTACTTCCTAGCGTTCCACAACGGTGGGGAAGTGATTGGTGATCTTGTCAACGAAGGTAAGTGTGACAAGGAACACGGTACCAAGGTTGAGTTTGTCCCGGACTTTACCGTGATGGAAAAGAGTGATTACAAACAAACGGTAATTGCTAGTAGGTTGCAACAATTAGCCTTTCTCAACAAAGGGATCCAAATTGACTTTGTTGATGAACGCCGTCAAAATCCGCAAAGCTTTTCTTGGAAGTATGATGGTGGCTTAGTCCAATACATCCACCACCTCAACAACGAAAAGGAACCTTTATTTGAGGACATTATCTTTGGTGAAAAAACCGATACTGTTAAATCAGTTAGCCGTGATGAGAGCTACACAATTAAGGTGGAAGTGGCCTTTCAGTACAACAAGACGTATAACCAATCAATCTTTAGTTTTTGTAACAACATTAATACCACTGAAGGCGGTACCCACGTTGAAGGCTTTCGCAATGCCTTAGTGAAGATCATTAACCGCTTTGCTGTCGAAAACAAGTTCTTAAAGGAAACGGATGAAAAGATTACCCGCGATGACATCTGTGAAGGGTTAACGGCCATTATCTCAATTAAGCACCCCAACCCCCAGTATGAGGGTCAAACCAAAAAGAAATTGGGTAACACCGAAGTGCGTCCTCTAGTTAACAGTATTGTTAGTGAAATCTTTGAGCGCTTTATGTTGGAAAACCCCCCAGGAAGCCAACGCCATTATTCGCAAAACGCTGTTAGCGCAAGAAGCACGGCGCCGTAGTCAGGAAGCACGGGAACTGACCCGCAGGAAATCTCCCTTTGACAGTGGTTCACTCCCGGGTAAGTTAGCAGACTGTACCACCCGTGATCCCTCAATTAGTGAACTGTACATTGTCGAGGGAGATAGCGCTGGTGGTACGGCTAAAACGGGGCGGGACCGTTACTTCCAAGCGATCCTACCATTAAGGGGTAAGATCCTCAATGTGGAAAAGTCCCACTTTGAACAAATCTTTAATAATGTGGAAATCTCGGCTTTAGTCATGGCCGTTGGCTGTGGCATTAAACCAGACTTCGAGTTGGAAAAACTGCGGTATAACAAGATCATTATCATGACCGATGCTGATGTCGATGGGGCGCACATCCGTACCCTCCTTTTAACCTTCTTCTTTCGCTTTATGTATCCCTTGGTGGAACAGGGCAACATTTACATTGCCCAACCCCCACTGTATAAGGTCTCTTACTCTAATAAGGACTTATACATGCAAACCGATGTCCAACTAGAGGAGTGGAAGCAACAACACCCTAATCTGAAATACAACTTACAACGCTACAAGGGTTTAGGGGAAATGGATGCCATCCAACTGTGGGAAACCACGATGGATCCCAAGGTGCGTACCTTACTAAAGGTAACCGTTGAAGATGCTTCAATTGCTGATAAAGCCTTCTCCTTGTTAATGGGCGATGAAGTTCCCCCAAGACGGGAATTCATCGAACAAAACGCACGTAACGTAAAGAACATTGATATCTAATGGCAAAGCAACAAGATCAAATTGACAAAATTCGTCAGGAATTAGCACAATCAGCAATTAAAAACATCTCCCTCTCCAGTGAACTAGAACGTTCCTTCATGGAGTATGCGATGTCGGTGATAGTGGCTCGCGCGCTCCCAGATGCGAGGGATGGACTTAAACCTGTGCACCGCCGTGTGCTCTATGGTGCTTACACTGGGGGCATGCACCACGATCGCCCCTTTAAAAAGTCAGCACGGATTGTCGGTGATGTAATGAGTAAGTTCCACCCCCACGGGGACATGGCCATTTACGACACAATGTCGCGGATGGCGCAGGACTTTTCTTTGCGTTACCTCTTAATTGACGGACATGGTAACTTTGGCTCGATTGATGGCGATCGTCCCGCAGCACAACGTTATACGGAAGCACGGTTGTCGAAATTAGCGGGGGAACTGTTACGTGACATTGACAAGGACACGGTCGACTTTGTCGCTAACTATGATGGGGAAGAACAAGAACCAACGGTTTTACCAGCTGCCTTTCCTAATTTATTAGCTAATGGTTCGAGTGGGATTGCGGTAGGGATGTCCACTTCCATTCCGTCTCACAACTTATCGGAGTTAATCCAGGGCTTAATCCTTTTAATTGACAACCCTGACTGCACCATTAATGATTTATTAGGTGTCATTAAAGGACCGGACTTTCCCACCGGAGCCAACATTATTTACACCAAGGGGATTGAAAGCTACTTTGAAACTGGGAAGGGTAATGTGGTCATTCGTTCTAAAGTCAGCATTGAACAACTACCAACCCGGGCTGCTTTAGTAGTCACGGAAATTCCCTACATGGTCAACAAGACCAGTTTAATTGAAAAGATTGTCGAGTTAGTCAAGGCTGAGGAAATCACCGGGATTGCCGATATCCGTGATGAGTCATCCAGGGAAGGAATTCGCTTGGTGATTGAAGTGAAGCGCGACACCGTTCCGGAAGTCTTATTAAACCAACTGTTCAAATCAACCCGTTTACAGGTGCGGTTCCCCGTGAACATGTTGGCACTGGTTAAGGGTGCACCTAAATTGCTCAACATGAAGCAAGCGTTAACGGTTTACCTCGAGCACCAACTCGATGTTCTGATTCGCAAAACCCAGTTTAACCTCAAGAAGTACCAGGAGCGCTTTCATATCTTAAGTGGCCTTTTAATTGCGGCGTTGAACATTGATGAGGTAATTGCGATCATTAAAAAGTCCGCTAATAACCAGGTAGCAATGGAAGCACTCCACGAACGCTTCGGGTTAGATGAAATCCAAGCACGGGCGGTTCTGGACATGCGTTTACGTAGCTTAAGTGTCTTGGAAGTCAATAAACTACAAACCGAACAACAAGAGTTGAAAGCTTTAATTGAGTTCTGTCAACAGGTGTTAGCTGACAAACAACTGCAACTCAAACTAATTAAGGAGCAGTTAACCAAGATTAACGAACAGTTTGGCGATCCACGCCGTAGTGAAATCCTGTACGGTATTAGTGAAGACATTGATGATGAGGACTTAATTACCCAGGAAAACGTGGTAATCACGATGTCCACTAACGGTTATCTCAAACGGATTGGGGTAGACTCTTATAACCTGCAACACCGTGGTGGGGTGGGTGTTAAGGGTTTAACTACCTACACCGATGACAGTATTAGCCAGCTGTTAGTCTGCTCGACCCACTCGGACCTCTTATTCTTTACGGACAAGGGAAAGGTCTACCGCATCCGGGCCCACCAAATTCCCCCGGGTTTTCGTACTAACAAGGGGATTCCGGCGGTTAACCTGATTAAGATCGACAAGGACGAGAAGATCTGTGCCTTAATTTCGGTGAATGATTACCAAAATGGTTACTTCTTCTTTTGTACCAAGAACGGTACCATTAAGCGCACCAGTCTAAGTGAGTTTGCCAACATCTTGAGTATTGGTAAACGCGCCATCCTCTTTAAGGAAAACGATGTTTTGTTCTCGGTTATTCGCACCAGTGGTCAAGATGACATCTTTATCGGTTCGACCGCGGGGTTTGTCGTGCGTTTCCACGAAGACACCGTCCGTCCGCTGTCCCGCGCTGCCATGGGGGTCTTGGGGATTAATCTCAACCAGGGCGAATTTGTCAATGGTCTGTCCACTTCCAGTAACGGGAGTTTATTACTCTCTGTGGGTCAAAATGGCATTGGTAAGCTCACCAGTATTGACAAATATCGTTTAACCAAGCGGAATGCTAAGGGAGTTAAAACACTACGAGTAACTGCTAAAACTGGTCCGGTTGTGACCACTACGACCGTCTTTGGTAACGAAGATTTGTTAATGATTTCTTCAGCTGGTAAGATTGTCCGGATTAGTCTGGAACAACTTTCAGAACAGGGAAAAAACACTTCTGGTGTTAAACTAATCAAACTAAAAGAGAAGGAACGCTTAGAGACTGTTACTATCTTTAAAAAGGAAGAAGCCATTAAAACCACAACAGCAACCGAGACAGACGATGTTGGATCGAAACAAATTACGCAATAACCTTGACTTCTTTAAAAAGAAGTTAGTTGAACGGGGTGTAAGTGAAAGCCAGTTCGAGGCGTACGTGCAAGCTGATAAAGCAATGCGCAAACTGTTGCACCAAATTGAACTGGCCAACCAAAAGCAAACCCTGTTAGCGAAACAGGTTGCCAAGAAGAAGGGTGATCTGAAGTTATTAAAGGAATCCAAGGAGTTAAAGCAAAAACTAGAACAGCTCAACATCGCTTTTAAGGAAGCGGAAACCTTAAGCCAAGAGTTACTGTTAAACCTCCCTAACATTGCTGATGAATCAGTACCAGTTGGACGGGATGAAACTGCTAACTTAGAACTGCTCAAAGAAGGGCGTAAACCGGTGTTTGACTTTACTCCCTTACCCCACTGGGAGTTGTGTGAACGCTTACAGTTAGTTGCCTTTGATAAGGCCACTAAGCTAACGGGTGCACGGTTTGTCGCTTACACTGATAAAGCAGCGAAACTGCTCCGCGCCATTGCTAGCTTAATGATTGACTTCAACAAAAACAAATACCAGGAGTGAAACGTGCCGGTCATTGTGAACGAGACTAGTCTTACTGGTACGGGTCAGCTACCCAAATTTAAGGATGATGTCTTTAAGTTAGAAAACACGCGTTACTACCTCTCCCCGACCCTAGAGGTGCAACTAGCCAACCTCCATGCCAACGAAATCTTCACGGAGGGTGAACTCCCCAAATACTATACCGCTACTGGGGTGAACTTTCGCCAGGAAGCGGGCAGTGCTGGTAAACAAACTAAGGGTACGATTCGCCTACACCAGTTCCAAAAGGTAGAACTAGTGAAGTTTTGTAAACCAAGTGAAGCGATTCACGAACTGGAAGAAATGACCCGGGACGCTGAGCAAATTTTGTTAGAACTCAAAATTCCCTTTCGCCGTTTGTTACTCTGTAGCGGTGACATGGGTTTTAGTGCCCAGAAAACCTATGACTTGGAAGTGTGGATGGCGGGTTGCAATGAGTACCGTGAAGTTTCGTCCTGTTCTTCTTGTGGTGACTTTCAAGCACGCAGAGCAATGATCCGCTATAAGGACTTAACCACAGGCAAGAATACCTATGTGGCTACCTTAAACGGCACCGCTTTAGCGATTGACCGCATCTTTGCAGCCATCTTGGAACACTACCAAACGAAGGCAGGGGAAGTAATGATTCCCCAAGCCTTGTTGAAATACTTAGACTTTGACAAAATTACCAAACCAAAATAAACCAATGAAACAAGGCGTGTTTGTGGCCATAGAGGGCGTGGATGGTGCGGGGAAAACCGTCCTCCTAGAAGCTTTTAAACAGCGCTTTCCCCAAAGCTTTTTGGGCTTTAAAACGCTTTTTAGCAGGGAACCCGGGGGCACACCGTTAGCGGAAAAAATCCGGGCACTGCTCCTGCATGAAGCAATGGAACCACTTACAGAAGCTTATCTGTTTGCGGCATCGCGCACCGAACACGTGCGCCAGTTAATCCAACCAGCGTTACAACAAAAACAGTTAGTGATAGTAGACCGCTTTGTGTGATCTAGTTATGCATACCAGGGCTTAATTAAAAAAGTCGGACTTGATGTGGTCAAAAAGCTCAATGCTGATGCGGTGGGGGATAGTATGCCCGACTTTACCTTTATTGTTGATTGTGACTTTGAGACTGCTTTAAACCGGATGGCCAAACGCGGTCAGGACAATTTATTGGACAATACTGTCAAAAAGCAAGCGGACTTTAATACTATGCGCCAGTATTACCACAGTTTGGTTGATAACAAACGCGTCTTTCTCTTAGACGGGCAAAACCAAACGGGTTGTCTGGAACAATTTATTGAACAATTAAGCCAATGTTTAACCCAACCCACGCTCTCTTAATTATTCAAAGACGGGGTAGCTATCTCCAACCAGTTTTAACGGAGTACCTAACCCGGGTCGTTTGTGAACAGCAAACGGGGTGTCAAACATGTCCGAGTTGCTTGGAAATTCTCCATGGTACTTACAACAACTTTTACAGTTTTGACCAAGCCAACCCCTTTAAAAGGGAACATGCCTTGCACCTTAGTGAAGTGTTGAACCGCCAAAGTGAATCCAACCAAAAACAGCTGTACCTGATTAAAAACTTAGAAACGCTCACCGCTACGGCCATGAACAGCTTGCTCCGTTTAATTGAGGAACACCCGGTGAATACCTATGGTGTTTTTACCACGAAAAACGAAAACATGATCTTACCCACCATTTTGAGTCGCGTCCAAAAAGTAGTACTCAAAAAAGCGACCCAGTTACCGTTCCAAGTGGACAGTAAGGACCAGGCGATTCTCAAAAGTTTCTTTAGTGTAGATGAACAGTTACAAGCATTGGACAACGGTAGTTTTACCCGCTTAAAAACGATTATTACGACACTTACCAATAAGAAAAACACAGCGAGCACGGTTCACAAGGCTTGGGTGTTGTTTAAACAATTAAACCAAACGGAAACGGCTCAAGTACTGAACTTTATGGTGGATTACACCAAGGACTTAACCAAAAAGGACCGCTTGTTAAACATGGTGCAAAATTTGGTGTTTAACCCACCGAAAGCAGCGTTGTTTGCTAACCTCATTAACTGATAAGATGGACACCAAACAAACCATGTTTGCCCTCGCTACTGCGCCCTTCAACAGTGCCATTCACATTATTAGGTTATCTGGTCCCGATGTTTATCGCATTATTAACCAAATAACCAATAAGGAAGTCAAACCGTTGGGTATGCGGATTCAACGCGTTTGGTTAATTGATCACAACCAGAAAAAAGTGGATGATGTGTTGCTGTTTAAATTTGTCGCACCTAACTCTTACACTGGGGAAGATCTGATTGAAATTTCGTGCCATGGCAGCATGGTAATAGTCAACGAAATTATTGGTTTATTGTTAAAGCACGGGGCGGTACAAGCCCAACCCGGGGAGTTTACGCAGCGCGGCTATCTCAACGGTAAGATGAGTTTAAACCAAGCGGCTTCTGTTAATAACTTAGTACTTTCACCGAATACAACCCTAAAGGATGTCGCTTTAAATGCGTTAGCTGGTCAAGTTGATGCCAGGTTAGAACCCTTAGTGGAAAAGTTGGGTCAACTAGTAATGCAAATGGAAGTGAACTTGGATTACCCCGAGTACACTGATGAACAGCGCGAACTGGTGACAATGAACCAAGCAGTCTTGCAAATAACCCAAATCTTAAACCAAATCGTGGTTGGTCAAGACCAGTTACAACGGCTCAAAGATCCGTTTAAGATTGCCATTATTGGTAATACTAATGTTGGTAAGTCCTCCTTATTGAATGCTCTGTTAGATCAAGATAAAGCGATAGTATCCGCCATTAAAGGCAGTACCCGTGACATTGTTGAGGGTGACTTTGCTCTAAACGGTCACTTCGTCAAAATCCTCGACACAGCGGGAATTCGCCAACACCAAAGTGCGCTCGAAAAAGCGGGCATTCAAAAAACCTTTGGCGCCATTAAAACCGCTAACTTAGTAATTTACCTTTTGGATGCCCGGCAGCCAGAACCTGATCCCAAGATTATTGCGCGATTAAAGAAGCTGAAGAAGGACTTCTTTTTGGTCCACAATAAAGCAGACTTAGTGCAGCAAAGCTTTCAGGTAAGTATTTCTGCCAAACAAAAACAGATCCAACCACTTGTCGATTTATTAACCCAATACCTACACCAGTTTTACAGTGTGGAGCAGAACCAGCTCTACTTAATTAGTGATTGGCAAACGATCCTGCTCCAAAAAGCAATAGCCGAACTAGAACATTTTTTAATTAAACAACAAAACTGCCTTTTCTTTGATATCCTAGTTGTTCATTTACGTGCTGCTCACGAATACATTCTCCAAGTCTTGGGTAAAAACACCAACTATGACCTGATTAACGAAATCTTTAAGCACTTTTGCTTAGGTAAGTAATGGAATACTTTGATGCGCACTGTCACTTAAACTGTGAACCCCTACTGGAACAGCACGAAAAGAGTTTGGCCAACTTTAGGTTAATTGGGTTAAAGGCCAATGTTGTGGGCACTAATTTAACTAACTCCCAAATTGCGGTGAATTTAGCTAAACAGCACCCTGATTTACTCAAGGCAGGGGTGGGTATTCACCCTAATGATGTCCAGCTTTTTGATTTAAAAGCAGCGCAAGCAACACTAGAAAAATTAGTAAGTACGCATCGCTCATTCATTAGTTGTATTGGTGAATATGGCTTTGATTACCACTACACTAAGGACTACATTACCCAACAAGAACAGTTCTTTTTGATGCAGTTCCAACTAGCAGAACAGTACCAGTTAGTGCACATGTTGCATGTCCGTGATGTGCACGAAAGGATATACGAAGTTTTGAAAAGGTTAAAACCTAAACAACCAGTGGTGTTTCACTGTTTTAGTGAAGACACCAATACTGCTTTAAAGCTGCTAACTTTAAGAGAAGTAGGACTAAAGGTTTATTTTTCGATTCCGGGGATTGTGACCTTTAAGAATGCGAAAAACTTGCAAGCCGCTTTATCAGTTATTCCAACAGAGCTACTTTTAAGTGAAACCGATTCGCCTTATTTAGCACCAGTACCTTTTCGGGGCAAAACTAATTGACCGGAATGCGTTGTTCATACAGTGCAAACGATTGCTGACATTAAGCAAGTTCCTTTAGCTGAAATAAAACAAGCGATTGTGCATAACGCCAAGAAGTTATTTTGGTAGTCCTTAGGATTAACTTTTAAAAGTTCTTCTTTTGTTGGAACCTTTTCACAACTTATTCAATTTATTTAGTGTAGACATGAATAAAAATGCATACACTAGAAAAAATTAGTTCCTGATTAAAGGGGAATCCTTATTTAAACAAAATTGTAAGTAGTAGTTTTCTCAACCTCTTTAAAAAGAAAGGAGGTATTGCTTAATGGCTTACTCGCCTTCGCTTAACGACATTAAGAGCATTTTGAACAAATATACCTCTAAGGACTATGAGTTAAAATGCGAAAATAGGTATGATGGCAAGCTTGAATTATGATTAAAAGGAGTTTTTGAAGAAATCGTGAAAACACCTGGCACTAGGTACGTCACCCATAAGCAACTTGATGAAAAGCTCAAAAATTTTGTCACTAAAACAGAATTTAAAGAGTTCCAAACTGTAGTTATGGAGAGTTTTGCTGTTCAAAACCAGAATATTGATGCTCAGGGTGAGCAGATCAAAGAACTTCAAGTTGAACAAAAAGCTCAAGGAAAGACCCTGCAATTGATACTTGAAGCTCTCCAGGGTATAAACAAACGTCTAGACAACTTAGAGTCCAAATAAAAAACTAAAGCAGTTTTTATACCAACAGTTGACTCTTAAAAAGTTTGCTGTCGGTATTTTTATCCACTTCTAACAAAAAATCATTAAAAAACAGGCGACTAAAAAATCTTGAAGAACAACGTTAGGTTGTTCAAAGAATTTTGAACAATAAAAAACAAAACTAAGTTGAATTTTTAGTTAAGCCATTACTCAATTAAATAGACTAAATATCTGAATAGATTAATTTGGTAGTTATCAACTATGTATTCAATATAACCATTAAGAACTGTTTTGTCAAGAATTTTTTGACTAAGTTCATCTTTGCCTTTTAGTATTCAATGAAACGAAACAGATAACCTAATTCTTTTGGAATAGACATCAAAATTTTTTGACGTAAATGGTAAATATGAATTCGCTTTAAGAGTAAGGAAACTGTTGAAATAATGTTTGTCACCTTTGTGGATATTGAATCCTCTGTAAGAATAATTTTCATTTCATGTGAGAGTATGGTTCAACTTGGCTTTAACTGAAACATCGCTAATGCCTTTGTATGTCATGTTGAAATACTCAACTTTTTGTTTCTTATTAAAAGTATTAACGCCTGAAAAAATCGATTTAGTTCAATTAAATAAATCAATACCGATTTCATTTTTTTCTGCACTTTGCTTTCAGGCATTTAGTAATAGTTCAGTAGTTTTTTCAGAGTTAACTTCGTAAGATTTTTTTAAATAACCTATTAAGATTTGTTTCCCTTCCGAAGTAATAATTAATTTAGAAACATCAAGGTCTTTACCATCTGTTTCCTTAATAAGATTTTTGAGATCAGCTTCGAAAGTGGCGCTACAAGCACTAAGAAATAAAACTGAAGATAATAGTGGGGTTAATAAGAATTTAAATTTCATTAGCGCACGTAGTAAATTACTTAATTAAGAAAAGCAAGTATCTAAAGAGATTAACTGTGTATTCAGAATTTGTTCTAGTATCATAGCCTTCCAAAAAGATTTTCTTAAGATCTCTTTGACTTTGTTTATCTTGCCTTGCAATGATCAACTAAGTGAAACAGGGAACTCAATTCATTCTGTGTCAACGCCTTCCCCTGTCTTAGTAATGAAACTTATCGTTTTAGATTGGTTCTTTGAAGATGGCTTAATTGCAAGAGGAAGATCAAAAACATGTTTGTCACCTTTTTTGGCAGCAAACCCATTGGAAGAATAATTTCCATGCCAATTAAAGTTATAGTTCAACTTAGCATTTATCGAAAAATTATTAAGATCTTGATATTCCATATCGAAATATTCAACTTTTAGTTCGATTTTTAAAGGATCATTGCCTGTTGCATAAATACCAACATCGGGAAAATTAATTTTCTCAATATCTATTTCACCTTTTTCCGCACTCTGTTTTCAGGCATCTAACAATAGTTTTGTGGTTTTATCAGGATTAACTTCGTAAGATTTCTTTAGAGAAGTTATTAAGTTTTTTCTGCCTTCAGAAGTTTTGATTACTTTGTACACATCAACGTCTTGTTTAGTGGTTTCTTGAATAAGATTTCTTAGATCAGACTGGATAGAAGCATAAGCACTAAACAATAAAGTTGAAGATAAAAACGGAATTGAAAGGTATTTAAATTTCATTGAGGTACAGAAAAACTAACTACGGCAATAGAACCTTTTTGGTAATGGTAAATTTTATCTAGTGTTTTATTGTTTTTCAACTTAATTCAGTAAAAGCATCCACATCTAAATAAAAAAGCCATCTGTTTAGGGCGAAAAGCAGATGACTAAAAATAATTTTTTAACGATTTAAAAGCTAACGGAGATTGTTAGTTTGATGTATAAGTAAAAATATGTTTTCTAAAAATTTATTAAGTTGGCCGGGAGCAGTAAAAACTTTTGATTCCAGCAGCTCTTCCTGCTCCCACTAAATAAATTGACTTGGTTTTACAAAGACGCCAAAAAAGTTTGGAGCTTTTTGTAATTTAGTTTTTTAGCTTCTTCTAACTATTAAGAAATATTTACTTTTCTCAAAAGCTTTTGAAAGGTTTTTGTCACTTCGCTATTTTTAAAGGCATTTAAGAGATGTAAATACATCGCTTATGTTTCTAAATACTCTTAAAAATTTATTTGGGTGACTTGGATCGCTCTTGATGTTGTTTTGTTTGAAGTCTCTTTACTTTAAATTGCAAAAATTTTTAGCAATTTTATTGGCAGTTTTTGAAATTCTGGTCAATTTATTTAGTGAAGGAAAAATTATGTCTTTACTCAATATATTTATTAGATCAACGATGATTTTGATCCTTGCTGGCAACAAAGTTGCACGTTTTAGTTTTCTAAACCTTGAGCAGAAAGGAGGAATTTATTAATGCCTAAAGTTATTTCCCAAAAGGAATTTAATATCCTTTCCCGGACAAAGGAAAGAGTTAATTTTCAAATTGTCTATACCAAGCAAAGAAATGATTTGCACAAAGCTGCTAAGGAGTACTTTTTTAAAGACAAAGACTTTACAATAATAGAAGATACACCGGACACACCTGACCAACCAGATGGACCATGTATTACTAAAGGCCCAAAGGAACCAGGTGGTCCTGAAGAGCCAAATGGTCCGGATAAACCAGAAGATCCTAAAGATCCTGATACGCCAGATGTTTCTGAAGGCCCTAAAGAACAAGGTGGTCAGAAAGATCCAGGTGGTCCGGATGATCCCAATGAAGGAAAGCAGAAAGTGTCCAAACCAGATAAATACGTAACCCACCGTGAGCTTGATGAAAAGCTTAAAGACTTTGCCACCAAAGCTGATTTCAAACGCGTCGAAGACAAAGTTGATGTTTTATTTGAACTGCAAAAAACGCAAGGCGAACAAATAAAGGTTCAAGGTGAGCAGATCAAAGCTCAAGGAAAACAAATTGAACAATTAACTGAAACTGTCAAAGTGCAAGGCGAGCAGATTAGGGCTCAAGGAGAACAAATCAAAGCTCAGAGCGAAGAAATAAAAGAAATTAAGGTTGAGCAAAAAGCCCAAGGCGAGCAAATCAAAGAACTTCAAGTAGAACAAAAAGCCCAAGGTAAAACCCTACAGTTAATTTTAAAGACGCTAGAAAAAATGAACGAGCGTCTAGAAAAATTGGAATCTAAATAGATAACAATCAAAGCAAAAATACCAGCAGTTGACCCTTATAGGTTATCTGTTGGTATTTTTATTTTGGCGCTTACTAGTTCTTGTAACCATTTATTAGTTAAATGGAACTTAGAAATACAAAATTAGAACAACTCTTTTACATCTATCACCGCAACCTGAAGTATAGATGTCCAATGCAGTACCTCACTAAAAGGGGCTTTAACTTACAAGACCTCTTGTCAGTTGGTGGTGGATTAGCTTACTTGGGCGAAAAACAGTGGTTAAATTTATCACTGTACAACTTTAACAACCAGTTAGTTGGTTTTTTAAGTCGGAAGGTAGGTTTTGAAAAGAAGTTCCTTTATTTACCTATTAATAAACCACCAAGTAAAAGTGAATCTTTTTTAGGTTTAAAACACTTACCAACGGAAACGAACACCATTTACCTAGTTGAAGGTGATTTTGATTGGTTAGCTTTCCGTAAGGGTGGTATTTTGAACTGCTTGCCTTTATGTGGATTAACTTTATCAGATAAACAAATGAAGTTTTTTCAACAAAGTAAGATCGAAAAAGTGGTGCTTTGCTTAGACAATGATTTTGCTGGTAAAGTAGCTGCTGCTAACCTGGAACGCATTTTAAAAAATGCTGGTTTTCAGGTTAAGGTAGTTCAGCTAAAGGGTAAAGTTAAGGACTGAAATGAATTATTACTACTTTACCCGAAAAACTGAGCTAAAGCCTTAAGGGATCATTTGGCGCTTTAAATGATGTTTAAAGTGAGTTAAGGACGATTTAATAATTACCCTTGGTAGGTTTAAGTCAGGACACATTTTGGTTAGTGCCCTAGCACCAGCAGCATCCTCGATTTCATTGACCACTATTCGGTTGTACTTATTTAAGAAGAAGTCAATGGCGTAGTAACCATGACCTAGCTTTTTAGCAATCCGTTTAATTTTGTGCTTCATGTATCACTTTAGTTTAAACAAATCAACAGCAGCATTTTGTGAGTAGTTAGCCCTAAAATCATCACCACTAGCAGTCCTTCTAATTGTTTTTAACACCTTACCAAACAAGATGTAAGCCCGGTATTCCACTTTACCAATTGACAAAAATGGTTGGACAATCCACTCATTAGGATTTTGAATTGGTAACTGCTTAATTTCTTCCGCATTTTGAATTAAGTGCACATCTTCCCCACCATGAGAATCACGCTTTTTGATCACAATCGGAAAAGTCTTAATCTTTTTGGGATCAAAAATGGTGGTATCAACGGTCAAGAACTTATGCTGCTTTAACCACCTGTAAGTTTCATACTTATCATTGGCTACCAAAACGATATTTTGGGGATTAATGAGAAAACTGTTGAGAAAGTTGTGTTTGGTTAAAAAATCAATTCTGCGCGAACGGTTAATGATCAATTCCACATTTTTAATGTGTTTGTGATCAGCGGTTTCATCATCTAACAGCAAAATATGCCCAACTAGTTTTTTCTTGTTCAGTTCATCGACTAGTGCTTGAGCAAAGTGCTTGTTCTTGTCAAAATCGGTCTTATTGTAAACAACGAGTGCTTTTCTGTTTTTTAACTTAATTTTGGCCATTATGCGATTGATGCTAAAAGTTGAATGGCGAGGTTAGTTTTCAAATACATCGAGCTTTTGGTGAGCTGTACATTGGAATTAACTTCGCAGAAAATTAACGAACGATCCTGGTCAAATAAAAAGTCAATGCCACAATAAAAGAGTTGCATTGCTTGGCTAATTGTAATTGCTAATTTGGTTTCTTCCTGACTTAGTTGATATGGTTCACCAACCGCTCCCTTATTTAAATTAGAGCGAAAATCACCCTCATTTTGAGTATGTAAGGCCCCAACTACCTGGTTATTGACCACAATTACCCTGACCGCTTCACTATTAGAAGTGGCAATGTATTGCTGCACAATGATCTGTTCAGTTAGCCCGCTTAAGGTTTGACGTAACTTCTGTTCATCTTTGCACAAAAAAACGTAATCACCAAAAGAACCATAGATACTTTTGACAATCACTGGAAACTGAAAACAGCGCTTAATTTGTTCGATGAATTGTTCCAACTTTTCCGGATATCAAGCTAAACGAAAGTTCTGTGGGCCAATTAATGTAGGAATCTGTTTAATGGTTGGGTGTTGCGCTAAAACAGCATGACTCAAGGCCTTATTGTCCGCGTTATTAATGGCAATCGAACTGTTAATAACGCGCAAACCAACTGATTCTAACCATTGCGCTAACGCTATGTTTTTATCCAAAAACAAAATGGTATTGGACTTAAAGGGTAGTTTGGTCTTATCCTGATTGGTATTAATATCAAAGCAAGTAAAGAAATCGAGTTGGATGTTTAAGCTCTGGGCTGCATCCTTTAACAGTTCCGTTTGGTTTAATTTGGAAGCAATGTTAGTAAAAGCAGGGTTATATACGGCGCTGATGTGCTTCATAAAGGACTCAAAGGTTTTTCATAATGGCTAACACAGTAAAGGGTCCTACCCCTCCAGGTGTGGGGCTTCACCATTTGGCAACTTTCTCTATCCCATTAGGATCGATGTCACCACACAGTTTGTTTAAGGCATTACGTGATACCCCCACATCATAAAGAATAACACCTGGTTTAAAGTTAGTGGTCTTAAAGAAATGGGGCTTGCCAATGGCTGTGATCACCACGTCAGCGGTTTTAATTAGCTCCATGGTGTTGGGGGTATGCTTGTCACATCCCACCACCTGATGACCCGTTTCCTTTAAATAGTGAAAGATCGGCCCGCCACCAGTGACGCCTAAACCCACCACCACAATCTGCTTGTTGGTGAAATCATGGTGAAAGTGTTCTTTCAAAATTAACACCGCTTCCACCACACAGGACCTAATGGGTAGGGGGCAGTTACCAGTTAAGTAATCCACATCCTTGTAAGGATCAATTGCTTTTAAGAGCTTGTTTTGTTTTAAATGGGGATACACTGGCAGTTGCAAGATAATCCCTGTTACCTCAGGATCAGTGTTGAGTTGTTGAATTTTTTCTAACAATTCTTTCTCAGTGACACTTTCGTGGTACTTAATTAGTTCACTCTGGGCCCCTAGAGTATTACAAGCATTCATTTTTTGGCGAATAAAGGCATCAGATGACACATCATTATTAGTTTGCAGAATGACTAATTTGACCTGACTTCAATCCCTGTTTTTGTAAGTTAAAAGGATTTCGTGTGCTAGTTGCTTGCCATCAAAGGACATCTCTTAAGTTGTTGTTTTTTGTCAAGCTAAACAAAAAACGCCTAACTTAATCTTATATGACTAGTTAGACGTTTGGATCTTTAATGGTGGAGATGAAGGGTCTCAAACCCTCAACCTCCTGAGTGCAAATCAGGTGCTCTATCAGTTGAGCTACATCCCCATTATTGGTGGAAGTAAATGGACTTGAACCATCGACCTCACCCTTATCAGGGGTGTGCTCTAACCAACTGAGCTATACTTCCAGGCAAAATCTTCGTACAGGTTCGCTTCATAATTATATTAATTTAGCCAAAGCTAGTTTTTAAGCGGTCTTATAATTAAAGCAAGTAGTTTTTATGGGTCTTGTTTTAAAACAATTTAACAGGAAAATAAGAACAGCTTTAATTCTGGCGCCGCTTTTCACCTTTGCACAGATTATTATTGATCTTATTATTCCGTCCTTTTTGGCCAGTGCTATTGCCGTAGTGTTCTCAATTGTCACGCTTAAACAAAAAGAAGCATCAGGCGAGGGAGTTGCTGTTGATTTTATTGCTGAAAGTAAGTTAAGTTTCCAAAGTGTCCAGGAAGCGCAAATAGTGTTAGCGACCTCAGTGATCTTGTTAGCACTCTTTGGTTTAGTGTTTGGGTTAATTTCCATTTTTTGTGCCAGCATAGTAGCGGGGAATACCTCTTACTTTTTAAGAAGGAAGATTTTTCGCAAGATTATGCACATTACCGCACCTTCTCATGACCAATATGGCTCTTCTACGTTGTTGGTAAGGTTGACCAATGACGTTTACTTAATGGAAATCATGACCTTTGATTTTTTGCGGTTAATTGTTCGTGCACCTTTCTTGTTTATAGGGGGTCTAGCCTTTGCTATTGCCACTAACTCTGACATGTCGATTTCGCTAGCCATTACCTTTCCTTTGACCTTCTTAGTGATTGGGATTCTCAACAAGAAATCGACCCCGTTATTTAAGAACAACCAGAAATCGGTGGACCAAATTAACGAACGCGTGGAGGAAGATGTGTCGGGTTACAAGGTAGTGCAGTCGTTTAACTTAAAGGACACGGAATGTCTCAAGTTTAAGGCAGCTAATGCAAGGTGAACTAAGTCGAGTACGAATTCGTTGTTTGTCAATACACTGAACATTCCCTTTACCTTCTTCTTTTCCAGTATGACTATCATCATTGCGTTGCTTTTAGTCTTCCAACTAGATAATTCAGTCAGAGTGGATCCCCTACCAGATAATGCTGCCATTAGACCCAGCATCTTTGCTTTCTTCCAGTACAACTTCTATATTGTCTTGGGTTTAATCTTGACCTCGCTAACAATGGTCAACTTTACCCGTTCCCGCGTGGCCTTGGGTCGGATTAAGGATGTTTTGAATAAACCGGAAATCCAACAACATGTGGTTCCTGATCAAACAGTTCTAGCCCCTAGCTTGGAATTCAAAAACGTGGCCTTTGGGTTAGGTAACAAGGAAAACCGGGACTTCTTGCAAGACCTTAACTTTAAGTTTGAGGCCGGTAAGACTTACGGAATTGTGGGACCTACTGGTTCGGGTAAGTCTTTAATAGCTAATATTATTGGGGGTTTATATGAACCCAACCAAGGTGAAATCTTTGTTGGTGGTCAAAGTATTAAAACGATTGACAGTGACTATTTAGCCAAGATGATCGGGATTGTCTTTCAGCAAAACATTCTCTTTAAGGGCACGATTGCTTCCAACATTAAGATAGGCCTGGAAACCAGGGAAGACTGGAAGCACGAACCAGATAGCAAAAAGGATGCTGCCATGAAACGCGCTGCTGCCATTGCTTGTGCTGATACCTTCATCGAAAAGTTCAGCGATACCTATGACCATACGGTGGAACAGTTAGGCAAAAACCTCTCTGGTGGGCAAAAACAACGCGTCGCCATTACTAGAACCGTTATTACTAAACCACAAATCTTAGTGTTGGATGACAGTATGAGTGCGCTGGACGCTTTAACCGAAAAGAAGGTCCGGGAAAACATTGCCAACGAACTTCCGGGCACCACTAAGATCATTATTAGTCAAAACATTAATTCGATTAAGTACGCGCACAAAATTATGGTGATTGATAACGGTCGGATTGCTGGGTTTGACAGTGATGCTAAGCTCATGCAGAGCTGTGACATCTATGTCAAGATGAAGCAAGCACAAAAAGACCAAGGAGGTGATTTCGATGCTGTCGCATAACCAAAAACCCAATTCCTGGAAGATTTTGTGACGTTTAATTAAGTCCGTGCAAGGTCGCACTTCCTCCAAGGTGCTGTATGTCATGGTCTGTGCCATCTTTGGTATTCTCACTGGGGTTACTAACTCGATTCTCTTGGCCCAGGGCCTGGGTTTTATTTTTCCTACCACTAATACCGAAACTGATGGTATCCAATCAGTGTACTTACTGGTATTTGCGCACAACTTACCGGTCATGGAGCGCTTGACGATTGTTTGTGTTACCGTGGTTGTGGCTTACATTCTCATCTTTAGTTTTAACGTAGCGCAGAACTATTTAGGTTTAAAGCTGTACCAAGAGATCTGTGCCTTGTTGCGCTGAAAGGCTTACCTCAAGATTCAAAGTATGTCCACTAGTTTCTTTGATACGCAGAACAACGGGGATTTAATGAGTCGCTTGACCAACGACGTTTACAACATTAACAACCTGTATGCCCAAGTGGGTGGACAGACAATTCAGAGTTTGTTTATCTTAATGACAACAGCGACGATTCTGTTTGTTTTAAGTCCAGTCATTGCCTTAATTTCGCTAACAGTCTTAATTGCTCTAATTGCCCTTTCCTTTCTCTTTTTAAAGAAGGCACGCGCTGCTTATGCAAAGGTGCAAAACAACTTAGGGGACATGTCGGGTTACATTGAAGAAGTGTTGTCGAACCATAAGGTAGTTCATGTCTTGAAACTGCAAGAGGTGATGATTGATAACTTTGACAAGTACAACCGCCCGATGGTTAACCCCACCATTAAGGCCAATACCTATGCGGTGTTTATTTACTCCTGGTTTGGTTTTATTTCCAATATTACTTACTTAGCTTCGATCTCAATTGCCACGGCCTTTAGTGTGAATAACATTCCCTCCTTTGGGGTGAGTGCGATTAACTATTCGTTTATGCTGTCTTACATTGCGGCCTTACGTCAAACAGCACTCCCTTTAAACCAGATCTTTAGCTTGTGAAACTTAATCCAGTTAGGGATAGTAAGTGGCGAGAGGGTCTTCAAGATCTTGGATCTAGAAAGTCCCCAAAAACAGGCCACCATTACTAAGTTACCCAACATTAAGGGCAACATCCGTTTTGAAAAGGTAGCTTTTGGTTACAGTGCGGACAAACCAATTTTAACGGGGATTGATTTCTCGGTTAAACACGGTGACATTGTGGCCATAGTCGGTCCTACTGGAGCGGGTAAGTCCACCATTATTAACCTGTTGATGAAGTTCTACAAACCGTTTGCTGGGAAGATCTACATGGACAACTTTGAGATTAGTGAGGTGAGTGAAACTGCTTGACGGGAAAAGATTTCGATTGTGCTCCAAGACCCCTTCTTGTTTAGTGGCACGATTAAGGAAAACATCCGCATGGGACGGCAAGATGCCACTGATGAGGAAATTATAGAGGCGTGCAAGGTGGCCAATGCTCATGACTTTATTATGCGTTTACCCCAAGGTTACAACACCTTTATTTCCAACAAAACTGATTATCTCTCGGTCGGTGAGCGCCAACTGTTAACTATTGCCCGCGCTGTTATTCGTAATGCCCCGGTGTTATTATTGGATGAAGCCACGAGCTCCATCGATGTACACTCCGAAAAATTGATCCAACAATCAATAGGCAGACTAATGAAGGATAAAACATCGTTTATAATATCCCATCGACTCTCAATCATCCGTAATGCGACGCTGATAATTGTGATTAATGATGGAAAAGTTTTGGAAATGGGTAACCATGAACAATTAATGCGGCAAAACGGTTTTTATGCTCGATTAAAGCGCTCTGCTGTGAAGTAAGATCCAACCATGACGATTGCTGAAATAAGGAAATTTGCACAAACCACGCAAAAGTTTGTCGAAGCGGAAAACCTGTTTGAGCATGGTAACGTAGTCTTGCCCAAAAAATACCTCAATAAGGCGCGGGGCATGGCTGAAGTATTGTACAACAGCCAGGTCATTAAAGTAAGTTTTACGGCTAAAGATGGTGAGCTAACGTGTAAGTGTAGTTGTTTGGCTAACGTTGACAACTGCGTGCACATTGTAGCTGTATTGCTCAAATACCACCAAATGCTAGTGGAGTCCAAACGCAGTTTTAACTTAGCTGAAGCCTTTCACTTGGACTGTGATCAGGCCGAAATGCTGATTGAAAACCTCAGTTTAGAGATTATTGCCGGGGGATGAAACTTTAAGCTGGGGTTCACGATTAACTTAGATAAGCACAATCCCCAACCGAGTGTCTTGCGCTTTTATTGTTGTGATGCGACCTTTGTGTACTTCCTTCACTTGGAAAACGACACTTTGCACTCAGTCGAATTGTCCAAGTTTAAACCAGAGGAACGCGCTACCTTACTCTTCTTTGATAAATTGTGCAAGCAGTTTACAGTGGGGTATGACCGCAACAGTTTGCTCTTTCCGTTAGCGGGTTTTTTAAAGGAGTTACAAGCCAACACGGAGCCCACCATCTTTGTCTTTAACGATGACAAAATTGACAACATCCTCTTTTTAAGGATTTCCAAAAAGCACCATGGTTTAAACCATGTTTGTGGTTTTAGTGGCAAGAAGGTATTTGACTTTGTTACTTATAAACAAAAGGAAAAGCAGATTGTGTTGCGCTCAGCGTACCTGAGTAAGTTCACTGACTTTAAGTTTCCTTACACCATTAACATCTACAAACTCCAGTTTGGGGAACCGCTGTTCTTTCACTTTTTAATTCAGCTAAAGCGTGATGGCTTTAAGAACTTCTACTTTCAAAGTGAAGACAGTATTGTCAAGGAAAAGGAATACCTACCGAAGCTGTACTTTAAGGTCGAGTACGATCCGGTCAAAAACAAGTTTGTCAGTGATGCCTTCTTTAAGTACAAGAACCACTTTAATAAGGGCACAACCACGGTGTATCCGCACCGCTATTACATGGCTAAAAAGTCGGATCGCGGTGGTTTTAACCGCTTGTTGTTTTACGAAGAGGCCGTGGAAAACTTCTACCAAGACCAGTTTGATTTGGGTTACTTCCGTAAGTTTGAACACCTACCGATCCAGGACAAAAACCAGATTGAGGCCTTTAAAGCAGCGCTAGATGACTTAATGCCCGTGGATTTGGCTGAAGTGAGTTTATCCGATAACTTACTCCACCAAAAACCGCTTCATTTTGCCCTGTCTGATCTAGAAGCAGTTGCTGTTGATGACAAACAAATTAAGTTAAGTTTTGCCCCTAGTGCGGTTGAACTGAAATTAATTAAACGGATCTTATCCGCTTACCATAAGGGCAATGTGGTCTGTATCGACCAGGAGAGTTGGTATGACCTCAAGCAACCAGCTGCTAAAGAACTAATCCAGTTCTGAAACCAGTTTGACTTACGCAACGCTACCAGTGATGGTAATCACATCTACCTCCCGAAATATTACTTGTTTGAAGTGGCCAAGATCTTTAGTCAGTACTTGGACATTAAAAACCTGTTTGATGTACCGACCATTAAAAAGATTGAAGACCAAAACAACAACGTCTTTGACCTTTCCTTAGAACACAAAAAGATTACTTCACTGCGCAACTACCAACAAGAAGGAGTGAAGTGAATTCGCGGTTTGGAGGAAAATAAGTTTGGTGGTATTCTGGCTGACGAAATGGGGTTAGGGAAAACGGTGCAGGTGATCTTTGCGCTGTTGGACAGCTATCTCAAAAACCACGTTAACCTCCCTAGCTTAATTATTGTTCCGGCTTCTTTATTGTTGAACTGAAAGAGTGAGTTTGAAAAGTTTGCCCCGCAAATTAAGGTCAAGGTAGCTAACATCCCTTCTAAGGAACGCGGTGAGTTGTACGAAAAACTTACTAACGAAATCCTGATTGTGAGCTTTAACGTGTTGCGCAGTGATGTGAAGTTAATTACCAAACAGCGCTTTCACTATGTTGTAATTGACGAAGCGCAGGGCATTAAAAACGACAGCTCTTCAATTACCAAAGCTGCCAAAAAGGTGAAGGGCAACTTCTGTTTGGCCTTAACTGGTACGCCAATAGAAAACCGCTTGTTGGACCTTTGATCCTGCTTTGATTTTGTGCTCCCAAGCTTTTTAGGGAACAAAAAGCAGTTTACCGACCAGTTTGAAAAGGAAAAAACCGACCAAAGCTTCCATTTGTTAATGCAGCGCACTAGTCCCTTTATTTTGCGTCGTACCAAGTCCAAGGTCTTGAAGGAACTGCCTAACAAAATTACCACCGATATTTACGTAGAATTAAACCCAATGCACCAGAAGCTGTATGAGGAAGAACGTGACCGCGGTTTGGAGGAAATTAAGCAAATCCAAGACAAGAGTTCCTTTAACATCTTGACTTTAATCTTGAAGTTAAGGCACTTGTGTAGTTTACCCAAAAACAGTCAGGGTATTTTGGAAAACAGTGCCAAAAAGGAAGCTGCTTTAGAAATTATCCACGAAGCGATTGAAAACCAGCGCAAGATTATCCTCTTTACCCAGTTCATTGATGTAATTGACCACTTTAAAGACACCTTTAAAGAGCAGGGCATTGAGTACTTTATCTTTGATGGCCGTAAGTCTCCTAAAAGCCGTCACAGTATTATCGAGAAGTTCAACAATGCCAAAAACCCTTGTGTGTTGTTAGCGTCCTTGAAAGCAGGGGGTGTGGGAATTAACCTCACTGCCGCTGAAGTGGTAATCCACTTTGATGTGTGGTGAAATACCGCCGTGGAAAACCAAGCGACTGACCGGGCCCACCGCATAGGACAAAAGAAAACAGTCCAAGTTTACCGCATTATTGCTAAAAATACGATTGAAGAGCGGGTCTGTCAAGTGCAGGCCGAAAAACAGGAGTTAGTGAGCAAAACGCTAGTGGAAGACGTCAATTTTTTCGAATCCTTGACCAATGAGGAATTATTACGTCTTTTTGAATAAATCAAAGCAGTTTTATAATTAACACTCGAATGATTAAAGTGATAAATGGCCGCTGGTAAACGTGATTATTATGAAGTGTTAGGCGTTTCCAGATCCGCCACCGCACAAGACATCAAACGAGCTTTCCGCAAGCTTGCTATGCAGTACCACCCCGACCGCCATAAGGGTGAGGGTGAGACTGTCCAAAAACAAAACGAAGAGAAGTTTAAAGAGGTCAATGAGGCGTACGAGGTCTTAAGTGACACCGAGAAGCGTGGTATGTACGACCGCTTTGGCCATGAAGGGTTAAATGCCTCTGGCTTCCATGAAACTGGGTTTAATCCCTTTGACATCTTTAACAGTGTCTTTGGTGAGGGCTTTTCGTTTGACATGGACGGGGGTTCACCGTTTGACTTTATCTTTAGTAGGGGCAAAAAGTCGCGGCAAAACCGGGTTGTTTTGCCCTATGATTTAGAGATTGCGGTGGGGGTAGACATTAGCTTCTTTGAGATGACTAATGGTTGTACTCGGACGATTGAATATACCAAACGGGTGACCTGTAGTGCCTGTGATGGTTTTGGGGCTGAAGGGGGAGAAACCGGGATGGTTAGCTGTAACAGTTGTGAAGGCAATGGTTTTATTTTGAAGAATCAACGCTCCATCTTTGGTACAGTCCAGTCCCAGATGTTGTGTCAGAGCTGTGGTGGTCAGGGTAAACAGGCCAAACACAAGTGTAAGACTTGTAAGGGAAGTAAGTACAAGAAGGTACCAACTACCAAGGAAATTCAGATTCCCGCTGGCATTTACCATGGTGATGCGTTAGTCGATGACCATGGTGGTAATGAATTCGGGGGTCACGTTGGCAAGTTAGTGGTCCATGTGGGGGTACTACCGAGTAAGATCTTTAAACGGGTTGACCATAATGTAGTAGCCAATGTTTTAGTCGATCCGATGATTGCTATCACCGGAGGGAAAATTGAACTCCCTACCTTAGAAGGCATTAAGGAATTCAACTTACGTGCTGGTACTAAGAGTGGCGAGCAAATAGTAATCCCTGGTGGTGGGATTAAATTTACGAAGAACTTCCGCAAGAAGGCCGGGGATCTCATTATTATTATCAGTTATGCCCGTCCAAGTGAATACAGTGCGCCAGAGTTGCGCAAGCTCAAGGAATTTATTAAACCTAATCAAGAGGTAAAACAATATTTAAACGCACTTAAGAATGAATACAAGTCCTAAGCAAAGTGGTTATTTAAAGGTTGGTAATGGTCATGAGGTCTACTTTTGAACTGCTGGTAATCCCCAAGGAAAATCAGCACTTTACGTTCATGGGGGACCAGGTTCGGGTACGGATGCGGGTTGTTTAAAGTACTTTGACTTAGACACTACCTATGTAATCTTGTTAGACCAACGGGGTTGTGGTCAAAGTAAAGCGGTTAATCCCTTACTGCACAACACTACCCAAGACTTAGTTGGGGACTTGGAAGCGTTGCGCCAACACTTAAAACTTGAGCGTTGGACCCTATTTGGTGGTAGTTGGGGTTCTACTTTAGCCTTAGTGTATGCCATTACCCATCCCCAAGTAGTCGAACAAGTCTTTTTAAGAGCGCTCTTTTTGGGCCGTGAACAAGACTGGGCTGAAATGCTGTTGGGCTTGGGCAAGCTCTTCTACCCTTACGAACACCAAACGTTACTGAAAGCCATTCCCCAGGCTTGTCGCACTGACTTTACGAAATTCACTAACTACTTTTATGAAGTGTTGCAAGGTAACGATTCCGCTTTAAAAACGCAGTTAGCCAACGCTTGGGTTAAGTGGGAAAATACCTTACTATCACCGATTAGCTATGTCAAAGACGAGAAAGCAGAAGATGCGAACTTTACCTTTAAGTTAGCGCTCTTGGAATGTCACTACGCAAAGCACCACAGCTTTTTAAAACCGAACTTTATCCTGGAAAATGTTGCGGTATTAAAGGACAAACCGGTGCACTTAATCCACGGCCGTTTTGATCTAGTCTGTCCGTTAAGTCAAGCCTTGGAACTCAAACGTGCTTTACCAACGCTTAACTTGTATGTAACTAACAATGCCGGGCACAGTGGCAGTGATCCCAATAACCTCACTACCATTAAACACCTTTTAAAAACACAACTGTAATGAAACGGTGTTACATTACCACCCCGATTTACTACGCCTCGGGTAAGCCCCACATTGGCCATGCCTTTACGACCATCTTAGCGGATGTGATTAAGCGGTATAAACAGCAAAACGGTTATGAAGCTTACTTTCTAACTGGTACGGATGAACACGGCAACAAAATTGAAAGTAAGGCCAAAAGTTTAGGTCTAGATCCCCAAACCTTTGTGGATCAAAACGTGGCTTACTTTCAACAAATGTGAAAGCAGTTAGACATTAACTTTGACCACTTTATCCGCACCACTGATCTTAGTCATAAAGCCCAAGTTCAACAGGCCTTTCAACTACTTTATGACAAGGGGTTAATTTACCAAAGTAACTGGGAGGGTGCTTACTGTGTCGAGTGTGAGCAGAACTATTTCACTTATGATAAACAAACAATGTTGTGTGAAATAGGTCACCAGTTAACCCTAGTCCAAGAACCATCTTTATTTATTGCCTTTAAAGATAGTAAGGACTGGATTGGGGAAATGATTGCGACAAACAAGCTCAACATTACCCCTGAATCCAGAGCCGCTGAGTTAAAGAACAACTTCTTGGATGGGGGTTTAAATGACTTAGCTTTAACACGGCAAAACGTGACTTGGGGAATTCCCGTACCCTTTGACAACAAGCAAACGATTTACGTGTGGTTTGATGCCTTGTTTAACTACATTACTAACTTAGGGTTTGCCCATAACGACCCTAAATTTAACAAATGGTGGAACAATAATGATGAGGAACACGAAGTCATTCATTTAATTTCACGCGAAATTACCCGTTTCCACTGCATTTACTGACCAATCTTTCTCCACCAGTTGGGTTTTAAACTACCGACCCAGTTTCTCTCCCATGGTTGGATTGTCGATGGTAATGGGCACAAGATGTCCAAGTCGTTGGGCAATGTCATTTCCCCCGAAGAACTATTGGCCCAGTTTGGTGTTGATGGTACGCGTTATTGCTTACTTAAAGAAATGCGCTTGGACAAGGACAACCGCTGTAGTATGGCGATCTTTAAGGACATTTACAACGCTGATTTGGCCAACAGTTTTGGTAACCACGCATCACGTACCTTTGGCATGATCAAAAAGTACTTGGGCGGCCAGTTAGACTTTATCGAAGTCCAAGACCCTCAAGTAAAGCAGCTAATGGACCAAGCGGACCAAGCGATGGTTCAGTTTGACACCGCTTGAAATAACTTCCAGTTTTACAAGGGCATTAATGGCTTACTGCAACTAGTCTTTCAAGCTAGCAAGTTAATTGACCAGTTAAAACCGTGGGAGTTAGTCAAACAAACAGATTACACCTTACTCAAGCAATTACTGTTTGCTTGTGTCCGTTGTACCCAAGTGTGCTTTGTCTTGTTAGCGCCGATCTTAGTTCATACCAGTACCCAGATATTTGACCTGTTTAACTTCAGTGCCCAAGCACGTAGTAAGACCCACCTAGCTGATCCACAACAACTGCAAAAAATCAGCTTAGCACCTGTAATTCAACTTTTATTTAAAAGATTGGATTAATTAAATTAGATTAGAATTTAATGACTATGCAAGTAGAATACCTCGATCTCATCTCCCAAGCGAAGGAGATTGCTGAGACCCAATTTAAGGCGGAACCGTTTTCCTTTGATGCTATTTGAAAGGAAGTAGTGAAGCACTTTAAGATCTCCAAACAAGACGAACCTAACCTAATTTCGCGGTTTTACCAAGACTTTTTGGAAGACCCTAACTTTGTTTATTTAGGGGAACGCAACTGGAAGTTAAGGGACTTCATGAAGTTTGATGAATGGAACAAGATTTCCCAAGCAATGTTTGTGACCAAGGAAATCTTTGAAGAGGGTTATGAAGACCTGTCCAACAAGAAGAAGGAAAACGAGGAAGAAGTGAATGACTTCATTATGGGCAATGATGGTGATGACAACAGTACCGGTGATGAAATTGTGCAAGGACTCATTAACGATTTTCGCGACGATAACCAATAATGCTAGTAAACATCAAACAAATGTTGCAACACGCTAAGCAACACCACTATGCTGTACCCCACATTAATATCAATAACTATGAATGGGCTAAGGCAGTATTAACAGCGGCTCAGCAGGCCAAGAGTCCGATTATTGTCTCCACTTCTGAGGGCGCTTTAAAGTATATGTCCGGACACCAAGTGGTAGTACCGATGGTAAAGGGCTTAGTCGATGCTTTAAAGATTACTGTACCCGTAGCACTCCACTTAGACCATGGTAGTTATGAAGGTTGTAAGGCAGCGTTGCAAGCTGGGTTTAGTTCCATTATGTTTGATGGCTCGCATTTACCCTTTCAAGAAAACTTCACTAAATCCAAGGAGTTAATTGAACTAGCTAAACAAACCAATGCTTCTGTGGAACTAGAAGTGGGTACCTTAGGTGGGGAAGAAGATGGTATTGTGGGTCAAGGTGAATTGGCCAACATTGAAGAGTGCAAGCAAATTGCGACCTTAAAACCGGATGCCTTAGCTGCTGGGATTGGTAACATCCACGGGCTTTACCCCGACAACTGAAAGGGTTTAAACTATGAACTAATTGAAGCGATTGCTAAAGCGACTAACTTACCCTTAGTGCTCCATGGTGGTTCGGGAATTCCGGAAGCGGATGTCAAAAAAGCAATTGGCTTAGGCATTAGCAAGCTCAACATTAACACTGAATGTCAACTAGCGTTTGCTAAAGCGATTCGGGAATATGTGGAAGCCAAGAAGGACTTAGATACCCACAATAAGGGCTATGACCCACGGAAATTGTTAAAGTCACCTACCCAAGCAATTGTGGACTGCTGCTTGGAAAAGATGCAGCTCTGTGGTTCAACCAATAAGGCTTAGATGTTAAGTGCGGGAATTGTTGGTTTACCCAACGTTGGTAAATCAACCTTGTTTAGTGCTATTACCAACCTGCAAGTGGAAATAGCTAACTATCCCTTTGCCACGATTGAACCGAATGCCGGGATAGTTAACGTAATCGATGAGCGCTTAGACAAACTAGCGAGTTTAATTAAACCCGATAAGGTTACCCACACTACCTTTCGCTTTGTTGACATTGCTGGTTTAGTTAAGGGTGCCAGTAAGGGAGAAGGACTGGGCAACCAGTTCTTGGCCAACATCAGAGAAGTTGACCTTATTTGTCACGTAGTGCGTTGCTATGAGGACAAGAAGATTGTCCACGTTAATAACCAGGTCGACCCCGTATTTGACTTTGAAATTATTGTCAATGAGTTAATCCAGGCCGACATTGAGGTGGTGAATACCCGGATTGGCAAGATTAAGCGCAAGGCCGAATCTGGTGATAAGCAGTCCAAGGAAGAATATCAACTGCTAGCACCCGTTTTACAAGGTTTGCAACAAAACCAGATGGTGTTGCATCTAGTGAATGAAGTAGATTTAAAGAAACTCAAGTCACTTAATTTACTAACCGCCAAACCGATCCTAGTGGTGGCCAATGTGTCAGAAGCGGATTTGTCCAACCTTGACCATAATCCCCACCTCACTCAGCTCAACCAGTTCTTAAAACAACATAACTTACCCCATGCCATTCCTGTTTGTGCATTATTAGAAAATGAGTTGAGTAGCTTGGATGCCAATGGTCGTCAAGACTGGCTCAAGGAGTTGGGTTTAAGTGATTACCAAGGTTTAAACCAACTAATTAAAACGGCTTATGATGCGATTGGTCTGTGGTCATTCTTTACCTTTGGTAAGCAAGAAGTGCGCGCTTGGGCTTTTAAAAAGGGTTGGTTAGCACCGCAGTGTGCGGGTGAAATTCACACTGACTTTGAACGTGGTTTTATTAAAGTAGAAGTAATTAGTTGAAACCAACTATACGAATTAAAATCGCTCCAGGAAGCCAAAAAGCAGGGCCTGGTCCGTTTAGAGGGCAAGGCTTACGTGATGCAAGATGGTGATGTGTGTCACTTTAAGTTTAACGTTTAGCAAGTAACTGCTGAAACGAGTAAACAACCCAACTTTGCTTGTGGTTGTAACTAATACATAAACAACACAACTCATAGTTAAAAAAGACTTCCACCTGCCCCTTAGTTAACTTGTGGGTTTTAAATCAAGCTAACAGCGCTTTTTGACTGTCGGAATAGGCATCAAAACCAATGTTAATTAGGTTAACGGTGAGGTTCTTAGTTGGTTTTAACATTTGGTTAACCTTTCACCCTGACCCTCAACCAAGATAGGGTTTGGTTTCTATGACTGACTCCCCTTTAAAGTCCTTGGTGCCAAAGTACACCTTCGGGGCATTGGGCTCAGCTTTCTTAAAGTAAAACAGGGAAGGAAAACCCAGTTTGTTAGCGAGGTGCCGTGACCTTTGGTTGGATTCATAAAACGATCACACTAATTCCCGCGGTTGCAACGTACGCTGGTAGTACTTAAGAAAGAAACTAATTGTTTCTAAAGCGACACCACTTTGTTGTTGACTGGGATCAATCCAAAACGATAATACCGGCATGTTGCCCCATTGCCCGACACAAAACCAACCCAAAAACTGCTTGTTTTTAAATAAGAGCGCTAACAGTTTACCGGGTTGCAGTTTGTCGTGGATGTATTCCAAGAACTGCTCAAAGTTTTCCACAATTTGCGCCATATAGTTAGTAGCACTTAAGGTGGTCCGGTGCTTCATGAAAACATTAAAAGCAGCTTTGACCTGTTTATTTTTGACCAACTTATTGGGGTTTTTCAGTGCCATTAGTTGGTAACGATCACCAAAAAAGCAAAACTCAAGAATAGTGTTTTTATCCATTTCAAAAGTCTTTTATAATCTTAAAGATAGTTGTGCAATTCAAGTATTTATTCACCGTGATTATTCCCACTTACAATTGTGGTCAATACATCCCAAAAGCATTAGATTCCTTGCTTTTACAAGGTGAATATTTTACTAAGACCCAAGTTTTAATCGTCAATGATGGTTCGACTGATAATACCAAGCAGATCGTAGAACCCTATACCCAACAATACAGTAACATTGAGTACTTGGAAAAACCCAATGGCAATTGAGGCAGTGTGGTCAACTTTGTCAAACAAAACCAGTTAGCTAAGGGTCAGTACATTACTGTATTAGACAGCGATGATTATTTTCTAGCTAACGCTTTTCAACGCGTAGCTGCTCACTTTGGCCATGACATGATTGTGAGCGCCTTTTACTGTTACATTAGCCCTAAAAGAAGGCGTTTTCTCAAACCATACTTTGGTAAAACGGGGGTAATTGAACAAAAGACTAAACTACGCACACCCCATTCCCAACCCTTGGCTAAGTTCTACCGCCACGAGATCTTTAACTTGCTAGATCCTTTAAAAGAAAAGCTGTTTTACCAGGACTGTCTGTTGTACCATAATGCCATCAATAAGGTGCAAAGTGTCTTTTACATTTGTGAACCCTTAGCAGTGTGGTATGCCACTAGACCAGGTAATTCCACCACTATGCCCTGAAATAATGCCGATAAGTTCCAGGCCTGGTGTGACTTGCTCAAACAAATGAACTTATACGGCGCGGGTATAGTGATTTACATTTACACTATGTTACCTGGTTTTTTAAAAGAATTAAAACGGCAACAACTAGTGTTAGATTTAGCTAAAAAACCCGCTTATACCTGATTGCCCCAACCCTTAGCATTTCTGTTTGGTGGCTTAATGGCACTCAGGACAAGAAAGTACATTCGTTACCCTAAGAATTAAGATTAAGATAGATAAAGCATATGGCAGACATGATTGAAGCGAAAAGTTTACGCAGTGGACAAACGATTTTTGGTCCCAATAAAGAGATTCTGTTGGTGTTAGAAAACACCTTTAATAAGACCGCAATGCGCCAGGGAATTGTTAAAACCAAGGTCAAAAACTTACGCACTGGGGCCATTGTTTGGATTGAGTTTACCGGAGACAAGTTAGAACAAGTCATTATTGACAAGAAGAAAATGACCTTCTTGTACAAGGATGGTGCTAACTATGTTTTTATGGACCAACAAGACTACAGTCAAATTGAGATCCCCGAAAAGCAGTTAGAGTGGGAAAAGAACTTTATCACTGAAGACAGTGAAGTTACTATTATTAGCTACCAAAGTGAAATTTTGGGCGTTAACCTACCCGAATTAGTGCCGATTGAAGTGGAATTTGCCGAAGAAGCAGTGCAAGGTAATACCGCTAACATGGCGCGTAAACGGGCTCGGTTAGTTTCGGGTTACGAATTAGATGTGCCCCAGTTCATTCGTACTGGTGATAAGATTGTGATTTCCACCATTGACGGGAGCTATCGTGAACGGTACAACAAGTAAATTAACTAGAACCCAAAGACGGATTGCAATTGTGGAGTTTATCTTCGCCACGCTGTTCTTTTTACCGAAAACTGCTGACCAAATCCAAGCGGCCTTTTTGGACTATGATGTGCCAGAACGTCCGCTCAACGATTGGCAAAAAGAGATTGTCAAAGTCTTTAGTGAACGTTGTGTGGAATTCATTGAACTAATTGAAAACCAACAACAACGCAACCAAGCAGAAGTACAAAGCAAGTACAACAAGGTTTCCGGTAAAAAAGTCGACTTATTGACTAAGGCCGTGATTCTCTGTGCTTTGAGCGAACAACACGCGCAAGCGACTGATAAACCATTATTAATTAGTGAAGCCTTACTGATTATGGACCACTATTCCCAGGTACCCGAAAAGAAGCAAACCCATGCTTTATTGGATAAACTCTTGTAATGCGACGTAATTGAAGGGAACACTATAACGTGTTTGTAGCAAACCTAGCCTTAGTGTTGGGTTTTATGTTGAACATTGTGGTGGCGCGGTATACCTTAACTGGTGCTACGCCCCAAGCACGTTTTTTATTCCTCACACCCTTTTTAGGCATAGTAGCAGCGAGTATCTTCTACTTCTTTGATGTGAAGTGGTTCTTAGCGGATTATCCCTACAAGAAGTTCCATTTTCAAAAGAAGTGAACCTGGACTTACTTAAGTGGCGTATTTGTTTTCTTTGCTAATATCTTGGTTAATGTGATCTTGTTAGCACTCCTCGTTAACCAGATGACCAACCAAATCCTTAGTGAAAAGTATACGGGTTTACTGGACAACGCTTACCCACTACTTTGATCCGCTGTGGGTGTTAGTATCTTTTTGAGCTTAATTAGTATTGGTTTGAGCAAAACAGCCCACTTTAAAATTGATGTGGAAATGCTCAAAGCGAAAAAAGGCGAACCCACCGCCGCTGATAAAACCGACAGCCGGCCGGTAGTTGTTGATTTAGACCAAACTAAATCAAAAAAAGATGGGGATAACCCACCCCAAGCTTCGGGTGATATGACTAGTCTTTAGCTTCGTTACCGAACAAGCGGTGGTTAATTAACTCGTTTAAACTAGTGTCATCAAACAAGGTGCAGACCGCAGTAGTTGCAAATGCCAAAGCACAACCAGCACTACGACCAGTGACGAGTTTATTGTGGACCGTAACTGGGCTAACATTGCGGTGCTTTGATCACTTTAAGCTATGATTGGGAAACGAGGAGTAGTTTTCCGTTTCACTAATAATGCCGTGCTTTTACAACACGTTGGGGGTATCACAAATGGCAAAGACATACTTGTCATGATCCTTAAAGTAACGGATAGTGTCGAGTAACTGTTGGTCATGGTCTAATACCTTGGTAGCTCCCATACCGCCAGGGATAAAGACACCATCAAATTCCTCCAGATTAACCTTGCTAGTAATCTGGTTTACTAAAGTTACCCCATTACTGCCCTTAACTGTACTTAAGTTAGGGTGATAAATCACGGTTTGTAATTCTTTAACAAAACCAAATACCACCATTGTGTTGGTGTATTCCACATCATTCATGTCAGGGTAAACAATGACTAAGAGTTTTTTCATCTTTTATTTAGTACCAAATAAGCGATCCCCAGCATCACCAAGCCCGGGAATAATGTAATTATCCTTATTTAATTTTTCATCAATGGCTGCTAGGTAAATGTCCACATCAGGGTGCGCTGCTTCCACGGCTTTAATGCCCTCTGGTGCTGCTACTATGGCCACCACACTAATCTTAATCGGGTGGTTCTTTTTAATGGAACTAATGGCTGTGGCTAGGGTAGTACCAGTAGCTAGCATTGGGTCTAAGATAATCACGTGGGAGTTGCCAATGTTTTCGGGCATTTTCTTATAGTAAGAAATTACATTAGTGCCCTCATGTTCACGGTAGATCCCTAAGTGACCCACACGAATCTTTTCCGAATAGCGCACAATTGGGTCAACCATACCCAGGCCAGCGCGCATTATGGGGACTAGAACAATTTCGTTTTTGAGCTTATAACCCTTCGTTTTGGTAAACGGGGTTTTTACAACAATTTCTTCTAAGGGTGATTCCTTAGTTACTTCAAAAAAGAGTAGTGAAGTGATTTGGCTTAACGCCATCCGAAATTGCGAGGAGGAAGAAGATTTATCACGTAGCTTTGTCAATTCGTTTTCGATCAAAGCGTGGTGCACTTTTTTGATCACGGTTAAAGATAGTCTTAATAAGGTTATATTAAAGATTGACTAAAAAAGTTTTTTCTGGTTGTCTTGCTCCCTAAACTCATCGAGTAGTTGCATTTGGGTAAACGCTTCAATGTGTTTTTTGGTAATTTTAGTGCGTTTTTTAAAGTCATCGAGCGAACTAAAGGGCTTTTCGTTACGCGCTTCTATGATCGAATTGGCCACAGCTTCCCCCATCCCCGGAATCGTAATAAAAGGGGCAATTAGCATGCCGTTGTGCTCGACAAAACGAGTGGCGTGGGAGTGGGTTAAAGAGATTTGTTCTAGTTTAATGCCGCGGGCCATCATCTCTAAATAAACCTCGTAACTGGTTAACAGTTCTGCTTCTTTGGGTTTAATCCGTTTAATGCGGTACAGTGTGTTGAGCTCCTCTAGTTTTTGCTTGACAAAGCTCACACCTGATTTAAAACCACTGACATCATGTTCCTTGAGTTTAAAGCTCAACAAACAAGCATAGTAGCTCAACGGGTGGTACAGTTTGAACCAGGCAATTCGTCAGGCCATTAAAACATAAGCCGCGGCGTGGGCTTTCGGGAACAGGTAGCTAATCTTTAAACACGAATTAATCCAGTGTTGTTCGACCCCATTGCTCTGCATTAATTGCACTTCCTTGGCATTGAGAGCAATTCCCTTACGCACCTTTTCCATGATCTCAAAAGCATCTTTGGCCTGCATCCCCTTATGAATGAGGTAGAGCATAATGTCATCGCGACAGGCAATTACATCCTTTAAGCTTAAGTTTTGGTCCTTCAAGATTTTCTGCGCATTGTCCGCCCACACGTTCTTCCCGTGACTTAGACCCGAGACGCGAATGAGGTCACCAAAGCCCTTTGGTTTGGTTTGTTCCAAGATCTTGCGAACGAACTTAGTACCAAACTCGGGAATCCCAATCGCTCCCGTGGGTTCATCTAAGTAGTGCGGTTTTAGTTTTAAGGGATTGACCGACCAAAACATTTCGGTGAGCTTCTTGTCAAACCGGGGAATGTTTTGTGGATTGACATGGGTAAGATCGGCCAGGTGCTTTAACATAGTCGGATCATCCTGGCCCAGAATATCTAACTTTAAGATGGTGTCCCCTAACGCATCATATTCAAAGTGGGTCGTTTTTCAATCACTGGAAGTATCATCAGCTGGATAACCACACGGGGTAAACTCATATACCGATTTGTGGTTGGGGAAGATCATAATCCCCCCGGGGTGTTGGCCGGTCGTGCGTTTAATCCCGACTAACTTTTGCTTAAAGCGTTCAATTTCGGGAGCGGTGGCCAATTCGGTTTGTTTAATAATTTCAAAGTAGTTGCGCGCATACCCGTAAGCCGTTTTTTCGGCCACTGTCGCAATTGTGCCAGCACGGAAGGTGTACTGTTCACCAAACAGTTCCCTCACATAAGCATGGGCTTTGGCTTGGTACTCACTGGAAAAGTTTAAGTCAATATCGGGAATCTTGTCCCCAGCAAAACCCATAAAGGTAGCAAAGGGAATGTTATGACCATCACCCTTAAAGGCTGCTTTGGCACCACACTTTGGACAATCCCTTACCATGAGGTCATACCCATCATCGACCTCTTCGTTGACCTCAAAGTACTGACAGCTCTCACAGAGATAATGCGGCACTAAGGGATTAATTTCCGAGATACCAATCAGGTTAGCTACCAACGAAGAACCAATTGAACCCCGCGGTCCAACAAAGTAACCATCTTGAACCGATTGTTTGACTAGTAAATGGGAAATCCAGTAAATAATGCCAAAGCCATTGTCAATGATCGCTTTTAACTCTTCCTGGATCCGTTGTTCTATTAACTTAGGTAAGGGATCACCGTAAATGGCCTTGGCTTGACTCCAGGTTTTGGCTGCCAGGTTTTCGTTAGAACCTTCAATTACTGGTGGTTGGAGTTTGGTTTGCACTGGTACCAGGTCATCAGCGGTGAGTTGTTTCGCTAACTTATTGGTGTTTTTGACCACTAACTCGTAAGCTAATTGTTCGCCTAAAAAACTCATTTCATTGAGCATTTCCCCTGTGGTACGCACAAACACATTGGGAACCCGTTGTTCCCTTTCCTTATAGTTAAAGTGACGGTGTCAACGCCCACCCAGACCCTTGGCACAAACTATGGCCTTATAGTACTCATTCTCCCAGGGGTGAATAAAGTAAGCATCCGAGGCAACTGCCACTAACTTATTGAGTCTTTGGGCCGTGTCCACCACTAACTTAATTGCGTTAGGAATGGCTTCTTTTTTCAAACCTTCCCTGATGGTGTAACCAGCATAAGCATCAGGTGGGGCAATTAAAACAAAGTCAACTCTTTTAATGGCCGCTTCTAATTCTTTAAGCGGTTTAGTCAATGCCGCTTTAAAGATATCACCTTGCACCGGGTTATCAGTAATGACAAAGGATTTAGCAAACTTTTGGAGTGTTGAAGTTAACACTAGGGGTCGAGTAGCGTTGTTATCGGTGAGCGCAATTGATAACAGTTCATACAGTTTAGCAAACCCACTTTGGCTCTTAATGTAGATAATGCCGGTATTGGTAAAAGTGCGCTTGAGCAGTTCAAAGTGGCACTGCTGGTTTAACTCTTGGTCTAACTGCTCGAGATTAGTAATCCCCATGAGTTCGACCTGTTCCTTGAAGTAGTCAAAGACCTTCTTTAAGGCCTGGGTATCATAATCCGCTCGGTGCAACCGTTCATCATCAAAGTCAAACTTGAGCTTGGCACAAATATTGCTCAACGTATGGGAGGCAAACCCGGGATTTAAGGCCCAGGATAAGGCTAAGGTATCAATTAAGGGATTGGTTAGTGGCGCAATGTTGTATTTTTCAAACTGGGTTTGTAAAAAGGGCAAGTCAAAACTCAAACCGTTATGGGCTACCATTACACAACCATCTAGTCAAGCGCGCAGTTTTTCCAACCCCGCTTGCTGTTCCATCCCATCTTCTAGTTGTGCTTGGGTAATCTTGGTAATTTCGGTAACTGCTGCAGGTAAGGGTCCATCAATCTTGAGAAAGAACTGCATTCGTTCCACTTCACGGTTATGTTTGACCTTAATTCCCGCAAACTCAATCACCGAGTCATACCGACCGTGTAACCCAGTTGTTTCAATGTCGAAGATGACAAAGGTCGCATCCTTAAGTTTGGTTTTATCAGGATTGCTAACTATCTTAATGTGATCATCAATGAGGTTACATTCCAAACCATAAATGGCCTTCAAGCCGTACTTTTTGGCCACTTCATATAAAGTGGGATAAATGTGAATGTTGTCCTTGTCAGTGACAGCAATTGTCTTCCAATCGCGTTCTTTGGCAAACTGGGCGTATTCCTGGACACTGTTAATGCCATCAAAGGCACTCATCTTGGTGTGGAAAACTAATTCGACCCGTTTTTGTTTGTCCTGATCGGGGCGGCGATAGTAAGCGGGGGTTTCAATCGGGGAAATTTCCTTAATGATACCCTGGAGTAGTTGGGTCTTGGCATCACGTTCGACCTTAACCGTGGCCTTAACTCAGGTACCAATCTTAATGCCCTCAATTGTTTTGGGATCCTTGTAAAACCACTTCAAAATTAACGAACCACCTAGTTGGAAATCAGTGACATAAATATTAAGGGTATTACGGAGGTTGTTGTACCCAGGAAAGTCCTTTAACTCAAAGATTTGCCCCATTAGTTTGATCTCATCCATTTGTTGGTTAATCTTGTGAATGGGGACAAATTCCGTACTAAAGACCTCGGTTTCAAACTGCTTGTCGTACTTGGATACCTTCATTGATTTCACTTTTAGTTGTTTTTCCGCTGGTGGATTAAACAACGAGACAAAACCAAAGTGGGTAAACCCAGCTTGGTGCATCCACTTAATGAAGGCTTGGCGTTGTTCAATTAACCATTGGTCTAATTCTTGGGACTGGCAGTTAGCTTTCAGTTCATGGTTACTGTAGGACAAGAAGTTAGGATGGGTTAACTCTTGCTCTAACAGGATGCGGTACTTCTTGTCCTTACCAAAGAAGCTTTTGAAGTAGTCTTTGATAATGGCTAAGGTGACACTGGACTTAGTGGACCCGTCCTTAAAGAAGGTGGCATCTTCTTGGAAGGTAATCCTCAGTTCTGCCCCTTCCACATCCTGAAAGCCCTCATACAGGGCGTTTCAAATGTCAATTGTTAACGGTTTTTTTAAAGTGAGCGCCACGAGAAACAGGGCGTTTTCGCGATCATTCTCGAGGTAAACCAGTTCAATCCGGTTGTTGAGTTGGTGCAATTCGTTGTGATCGAGAATATTTTTCTTAACTAAAAACTGTGATAATGCTAAAATGCGTTTACCCTTTTCGGTTTCGGTGTCGAATACCATATGTGCTAGTTCAAAGCTTATAAAACTGAAAAGGTAACAGCTTTATCTAACTGTTACCCGACGGTATTAGTTTACTATCTTGATCGTTTTGAAAACTTAAATGGTTAGTTAACTAGGTTCATTTCAACCACTACCTTTTTCAAGTTCCCGATTTGGTGTTCAAACCTAAAAAAAGGTTTGCTAAACTTAGGTGAGATTGCAAGATCTCGTTTAAATTTCCTCTGTTGCTAGTGGTAGTGTACCCAACTTTAATGCCATAAACGATTGCTATTTATTGATGAAAGCGTTCCGAAAATTGTTGCTGGGTCTCGCCTTACCCACAACCATCGGTCCCTTATTAGGCTTATTATTAACTAATACTGATACTGTCAAAAATGAAAGCTTAACTACTGTGCGCCACAGGTCCAGCATTAACCAGGACTTTGATGGCATTTTTCATACCGTTAAGTTATTAGAACCAATCCAGCAAAGTAATGCTGATCCCGCCGCTAGTTTTGCGCTCTTTCAGCAAAAGTTTCCCAACCTAAAACGTGTAGCCAACTCCACCTTAAATACCTTTGATGTCTACAACCTTTTGAGTGGTTGGAAGAGTAGCTTAACAGCGTACCTCAACCAGGTGTTGGCACTCCAACAACGGATTAAAGCAGCTGATCAAATTTTCCCTAACCAAAAGGAAACTTTACCCAAGGATGAGAATCCCAACATCTTTGAAGTGTTAGGTGCATATGGTGGTAAGGGTTTCTTTCCCACGCTAGGATCCAACGGGTTACACCTGCCACCACAGCTGTTCCAGTTCTTCCGTGACTTCCAACTAAGTAGCTTTACCATTAAAGACTTTGAGGTAGCGTTAGTGAGTGAACCGGACATAGTGCAACATGACAAAGTGCGCTATGCCTTTCAGGTGCAATTTAACTTAGTGTTACAATTACAAATTAACCGTAACCCAGTGTTATTTGACTTTAACCTACAGTTAAAGACGAATAACTTTGCCAATCAAACCAGTTTTGATGAATTGTTTAACGAAAAAACTAATCCACTTAACTGGCAGTTCTTTAGCAAGCTTAAAGTTAACCATTTAGTGTATGAAGGCAATGACATAACTCAGTTAGCTAATACCTTATTACAAAGCCAGTTTAATGTCTTACAACTTGATTTAAATAAGTCAATTTACCGCTTGAACTTAAACGCAATGGCACAGCGCTTTGAACACGACTGGGTACAACCGTTGTATGCCAAACGCACGCAGGCCAAAATTGCTTACGAAGCGGAACAAGCACGGATTGCGGCCGAGAACAAACGCAAGGAACTAGAACGGCAAAAGCTGTTAGCAGAATTGAAAGCCAAAGCTGAGCATTACCAAAAGATCAAGCAAGCACGGGAAAACATGCTAAAGGGCTTAAAGAGTATTACTGATTTTGTCAAGTTCTGAAAGAGTCCGGACCGTTTATTAGTTGGCTTTAACAAACAGGACGACATTACTACTCGTGCTGGGGTTTACAAGGCGTTACAAATTGCTTACGCTAACTATCCGCAGTGAACCTTTTATCTAACCCTACAAGGTTGGAAGAATGGTTCGGAGTTGTTGTTAAAAAGACCTAGTTGAACTAACTTATTAAGTGATATCCACTTCCAAAAGGCCTTTAACTTAAAGAACACCGTATCAGAACAAACCCTGGGTGCAGCTACCCTACCAGGTTATGGTTACTATGGCTTACGCATGAGTGACTGGTTGCGCTGGGCTTTGGGCTATTACTCCTATATCCATATGGGCGTACCGCAAAATGTACAGACCAAGTTTACGGGCACCCCAGATAATGAACAACAAGTCTGGATTGCTAACGAACCGTTTGAATGGAACAAACATTACGGTGTTGGTCCTAAATACAAGGATAAGGCCTACCGCTTTAACATGGAAATTAGTTTTGAACTAGAGGGTTGAATTGCTGTTAAGTGGTGAGCATGAGCCTTTAAGGGTTCAATTCCTGGGAACTGAAAAGGCAAACTCAAAGTAACCCATCTGTTTGATGGTATGGTACCAGTGTGGGAATTGGGCCCAGTCAATACCCATTTACCCCAATACAGCTTTACCGATCAGCAACAACTGTTGTTTGTACCCCACTCCATTCAAAAGATTGAAGCGATTGGTGCGGACAAAGGTATAAACGATCTTTTAAAAACCCAAAACCTCCATAACCTAGAGCGCTTGTCATACGAATCAACTAACCCAATTGACCTCATTTCTTACCTCTTGTACGCGATCCAGTACATTAAGGTTTAGTCACTTTCAGCAGTTAAAGTTCAGGTGTAAAGTTAACGATTAAGATCAAAAACCGTTCCTAAAAAAAGATCTTTTTCTAAAATCTAAGCGAGTTACAACTCAATTTAAATTTTCTCTCTGGTTGGTTCGCCAACAGTTTTAGCCACTTCAACATTCGTCAAACACCATTAAATGAAAACACTGCACAAGTTGCTGTTGGGGCTTTCCTTACCAGCAATCCTCGGTCCGTTGTTTGGTCTACTAGTTACAAAAACTGATGCTGAATCACCAGTTGCAGCATTACATAAATCGAAATCTTTTAGCAAAAACTTAGATAGTTTCCGCGCCTTATTTGTGGGCGAGGATATTGAGGAACAACTAGCTAAGGTACAACAACAAACACCAGAAGTTAGCTTTGCTAGCTTTCAACAAAAGTTTCCTGATAAGGCCAGTTTAAGAAACGGTTTTCAACCAATTGATGTTTACAACTTTTTAAGTGGTTGAAAAGGTGCTTTAGAAAACTTCTTGGCTAAGGTAGTTGAACTGCAAAAGAAGATTAAAGCAGCTGACGATATTTTCCCGAACCAAAAGAAAAATCCCGACAAGAAAGATAACCCAAATGTTTTAGAAGTGTTAGGTGAATACGGTGGAGATGGTTTCTTTCCTACATTAGGTAATAACGGTTTAAACATCCCGGACCAAGTTTTCCAATCCTTTAATAACTTCCAAATTGAGAACTATAAGATTAGTGACTTTAAGGTGGACATTGCCAGTGAACGTGACATTGTCCAACACGACAAGTTCCGTTTTAGTTATGTCGTTAACATTGGTTTAGAGTTAGCACTTTTAGTCAATAAGCGTCCGGTTTACTTTAACTTTAGCTTGGATCTGCGGACCAATAACTTCTCCAACCAAGCCGGTTTTAACGAAATCTTTAATGCGCCTGGTAACGCAGCTACTAACTGACAGTTCTTCAGCAAGATTAAAGTGAAACAGTTGAACTATGATGGTAATGACAGTACCCATTTAGCCAATACCCTCTTACAAGATCAGTTCAATACCTTAAACTTAAACTTGCAAAAGTCGATCTATGAGTTGCCATTAACCGAAATGGAAACTAAGTTCCAGCAAGAATATGTGGAACTTTTACTAGCTAAACGTAGAGAGGAAAAACGACTTTGGGATGAAGAACAAAAACGAATTGAAGCAGAACGGAAACAAAAAGAAGCAGAGCTAGCACAAATTCAGCGTGAACTTAAAGAAAAGGCTGAGAAGGATGCGGCGGTTAAACAAGCTCAAACTAACTTAAAAACTGCTCTAAGTAGTGTGGAAGGCTTTGTGAAATTCTGAACTGAAGGTGAAGACCGCATTAAGTTGGGTTTCACGAAGGAGGACAACCTTTATACCCGTGCAGGTTTAGTGAAAGCACTCAAAATTTCGTTTGCTAACTACCGCGCTTGAACCTTCTACATTACCTTGCTTGGTTGAAAAGCAGGTAGTGAAAAGTTACTTCGGAAGACTAGCTGAACTAACCTATTAAGTGACGTTAACTTCCAAAATGCTTTTGGCTTAAAAAATACAGCATCGGAAGAACAAGTTGGTAAAACATCATTACCAGGTTATGGTTACTATGGAATTCGCATGAGTAACTGACTCCGTTGGGCATTAGGTTACTATGCTAATACCCATGCGGGTAGTCCACAAAATGTGAAAGCCACCATTGATGGTAATCCAAGTGATACCACCAAAGTATGGATTGCACCAGAAGACTTTAAACAGGGTGTACACATTGGTGTGGGGGAACACTTCAAAGGTAAGGCTTACAAGTTTAAGTTTGAAGTTAGTTTTGAATTAGAAGGCTCGATTGCAGCCCACTGGTGAACTTTAGCCTTACGTGGTAACATCCCGGGCTACTGAAAGGGTAAACTCCAAGTAACCCACACTTTTGATGGTGATGTACCTTCCTGGTACTACGGTTCGGTACATACCCACGCTCCTGAATACCGTTTTACTGAAGATAACAAGCTATTATTTGTACCGCACTCCATTCAAAAGATTACCGCTGTTGGTGGTGATAGTAATGGTGTCAACGGCTTGTTGAAATCACAAAACTTACATAATCTCGAACGTCAGTCTTATGAGGTTACAGCACCAATTGATCTAGTTTCCTATTTGATGTTTGCGATTGCTAAAGAACCAACAAATAACTGGTAGTTCTTTGCTGTATATAATTAGCGCTTTATCTAGCACTATTTAGATTTCAAAAAAAGTCTAAGTGTGAATAAAAATACTAACAGCAAAACCTAATCAATATAGGCACTGTTGGTATTTTTATTGTTATTTGGAATTATTTAGATTCCAAATTGTCTAGACGTCTGTTTATGCCTTCAAGTGCTTTCAAAATTAATCTAAGTGTTTCTCCTTGATTTTTTTGTTCAACTTGAAGCTCTTTAATTTGTTGGCCTTGAACGATAACAACTTCAGTTAGTCGATCAACTTTGTCCTCAACAACTTGAATCCTTTGACCCTGACTCTTTAACTCAGTTTCAATCTTTGTAAGTCGTTGGTCTTTTGATTTTTTGTACTCGTTAAACTCTTTACGCGTTACGTATTCAGTGTTCACTGGTATAACCGGTTTGTTAAGTTTAAATTGTCTGTTGAAATTGCCAGAGAATTCCACACTGTTGTTGTCTTTGTCGATAATATATCAACCTGAAAAAGTACCCTTTTGGTCTTTTTCACCATGTCATGAAATTCTTTTTCGTTATAAAACGGAATTTTCTCTTTCATTAAGCCATACCTCCTTTCTTTTCAGTGAAATTTAAAAAGTAACTACTAATTGCAACTTTGTTGTCAACGAGGATCAACTGCTTGATTGATCAATATGCAAAATTATTTCTGTGCATAATATTGTCTCCACTAAATAAATTGACCAGACTTTAAAAAGGTGCCAAAAATTAAATCAAAAAGTTTTTAAGGATTTTTAAAGGAGTAAAAGTACCAGCAGCTAACCAATAAAGATTAACTGCTGGTACTTTTACTTTGTGCTTTATCTACTTAGATTCCAATTTGTCTAAACGTTTGTTTATACCCTGGAGAGCTTCGAGTATTAATTGAAGTGTCTTGCCTTGAGCCTTTTGCTCAATTTGAAGTTCTTTGATTTGCTTGCCCTGAGCTCTGAATTCTTCCTTAGTTACAAATTTTTCACTAAGTTCAACTAGAAGATCTTGTTTGAACACTTCTAGTTTTTTATCCAGTTGTTTATGGGTGACGTATCCACTTTTACCTTTTTCCATATGGTTTAAATTGCCATGAAATCCCATGTCGTAGCTACCATCAGGTAGTTCTTCTTCCCATGTATAACAATCATTAATCTTATCTTTATTTAGTCTTTTCTTAAACAAAGTAAGGGAAATTTTGCGTTTAATGGCAAAGACCTCCTTTATTTATTGATTGGAAAAAGGTGAAAGTGTTTAAAGAGGTAGGCACCAACAGGTCACTGAGCGAGTTGACTGCCCAAATAAAATAAATGATTTTTTAAAACTGTCATTTTATTACGCATACATCATTTAAATTACCCAAAAAACAAAAAGACGCCAAAAATTAGTGTGCAAATTTTACGAGTTTAAAAGTGTCTTTTTTAACTGGTAGAACTTTGTCTCAAAAATTAACAACAGTTAAACCGGCAAAAATAAAAATTTTGGGTTAAACTTTGGGGCGAGGTTTCCTCAGTTAAATATCCTTTTGGTTGGTTTTGCCAACAGTTTTAGTTGTCAGATTTTTTATTTGAGATTGCTAATAATTAATGACAAAACTCCCTAAATTGTTACTGGGGTTAACCTTTTCTGTCTCACTTATACCGTTTTCTAGTTTGCTTATTACTAGTACTGATGTCAATAAGCAACCGGTTCCAACTGCCTTACAACGCACTGGCGCCTCTGCCATTCATGAAGGTAGTTTTCAAACTATTACTTTAGGTCAAAGTTTAATGGAGCAAATTGAACAGCTCCAACAATTTACGCCAGCGCAGCGCTTTACCCAATTTAAAAAGAAATTTCCCAACCAAAAATTACTCAGTCAAAGTGAGTTAAGTCCAGTTGATGTTTACAATTTTTTAAGTGGTTGACAAGGTGCTTTGGTCTCTTTCTTGGATAGAGTAATTAAATTACAAGGCAAGGTTAAGGAAGCAAATGAAATTTTTAATCCAAATGTAGGTGATCAAATAGTTTTACCTAAAAAGGAAAACCCGAATGTTTTGGAAGTATTGGGCGAGTATAACGGTTTTGGTTTCTTCCCTACATTGGGTAAAAACGGATTAAACCTACCACAGCAAATCTTTGAAAACTTTACTGACTTTAAAGTAGAGAGTTACCAAATTAACGATTTCAAGGTAAGTCTAGTGGGCGAGCGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAACTGCTTGTTAACAACCAAAAGGTTACTTTCAATATTACGGTTGATTTGAGAACCAATAACTTTTCTACCCAAGAGACTTTCAATGATTTATTCAACAATGGAACTGCACCAACCAACTGACAGTTTTTTAGTCGAATAAAGGTAAATAAGTTGCACTATGACCAAACTGATGCCACCCATTTAGCTAACACCCTACTACAAGACCAGTTTAATGCGTTAAACCTTGATTTACAAAAATCGATTTACGACCTTAACCTTGATGGTTTAGAAGAAAGGTTTGAAGAGGAATACGCTAAGCCACTTCGTGAAAAAAGAACTCAGCAAAAGAAAGAATGGGAAGAGGAATAGCGAAGACGGGCAGAGGAAGATGCTAAACTGCAAAAAGAAATTGAAGAACAACAAAAGAAACAAAGAGAACAAGCAAAAAACGATGCTGAGCTCAAAAAAGCACAAGATGATCTCAAAGTTGCGCTTAATAGTCTTGACGGTTTTGTTAAATTCTTTACATCAAACGAAGGTCGCTTTAAATTAGGTTTTGAAAAGACAGATGACATTAAAACCCGTGCAGGGTTATACAAGGCACTGAAAGTTTCTTTTAGTAACTACCGTGCTTGAACCTTCTATCTAACTTTATTAGGTTGGAAGGAAACTACCGCAAAGATACTTACAAAAGCTAATTGAAAAGATATAAGAAGTGACGCTAATTTACGCAAGGCCTTCGGTTTAAAAGAAAGTGCCAACGAAAAAGAGGCTTCCAGGGTCATTACTCCGGGTTATGGTTACCAAGGTATTCACTTAAAACAATCGTTGCGTGAGGCGATTGATAAATACGCTACTTACAGCGTTTCTCGACCAAATAACGTCACAACTTATTTACCTGGCAAAGTTGATGGAAGTGGAAAAGATCAAATTTGAATTTCGCCAAACAACTTTCAAGTGAATCGGGAATGGGGTAATGGTGCACATTTTAAAGATAAAGCATATCGCTTTAATTTTGATGTCAAAGTTGAATATGATGTAGAGGTAAAAGCTGCTTGATGAACTGCTCTTTTCCGAGGTAGCATCCCAGGGTATTGAAAAGGTAAATTTAAAGTTACTTACTCCTTTAATGTTGAAGTACCTTCTTGAAACTATGGTGACAAGCAAGTGCGTCCACCACAATACAGTTTTAAGGAACAAGAAAAACAACTTTTATTTGTACCACGTCATGTACAAAAAATTGAAGCTGAAGGTAAACACCTTGAAATAATTAATCCGTTCTTAAAAGATCAACATTTAGATTTTTTTGAACACTACCACCCAGATTTAACGCAGCCTTTAGACATGGTGTCATACCTAATGTATGCCATCGCTGACAAGGTGAAATAACAAAAACATTTTGTCTGACAGTTGTAGCGACTCTTATCCATTTCTCAATTAATTATTAGTAATGAAAAAACTCCACAAAATTTTATTGGGTTTATCTTTGCCCGCATCTTTGGCGCCCTTATCTGGGTTTATTAGTACTGATGCTGATAACTCACAGTTTTCACTAAAGAAGTCTAGCACTGAAATTAAAGGTACACAATCCATTCACCTGCTTAATTTAGGGGAAAGTTTAACTGAAGAAATTAAAGAGGCACAAAAGCGTACCCCAGAAACTAGTTTTGCCAGTTTCAAACAAAAGTTTCCCAATAAGGAAAGTTTTGTTAAAGGTTTTCAACCAATTGATGTCTACAACTTACTAAGTGGTTGAAAGGATGCCATTTCATCCTTTTTAGATAAGGTTGTTGAGCTACAGAAAAAGATCGAAGAAGCTAATAAAATTTTCAACACTAATATTGGCAACCAAATTGATTTACCCGAAGATGAAAATCCCAATGTGCTAAACGTTTTGGGCTCATATGGTGGTGAAGGGTTCTTTCCTGCATTGGGTAAAAACGGGTTGAACCTACCACAACAAATCTTTGAAAACTTCACTGATTTCAAAGTTGTTAGTCATAAAATTCATGACTTCCAAGTAAGTTTGGTAGGCGAACGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAGCTGCTTGTTAACAACCAAAAAGTTACTTTCAACATTACGGTTGATTTGAGAACCAATAATTTCTCTACCCAAGAAACTTTTAACGAGCTGTTCAATAAATGCATTGGCCCCGTTAACTGGCAATTCTTTAGTAGGGTTAAAGTAGATAAATTGCACTACGATCAAACTGACGCTACTCATTTAGCTAACACGTTGTTACAAGACCAGTTCAATGCGTTAAATCTTGATTTAGAAAAGTCCATCTATGACTTGGAACTACCTAGATTAGAAGCAGAGTTCCAACAAAAATACGTTGATCCTTTAATTGAGAAAAAGCAAAGACAAAAAGCAGAGTGGGAAGAAGCGGAACGTATCCGTAAGGAAGAAGAGGAAAAACACCAAAAGGAGCTTGAAGAACAACAAAGAATTCAAGCAGAAAAAGCGAAAAATGACGAACAGTTACGTAAAGCGCAAACTGAATTGAAGAAGGCGCTTGGTGGCATCGACTCCTTTGTTGAATTCTTTACGAATAATGATTTACGTCTTAAACTAGGTTACACCAAAGAAGACAACGTTAGAACCCGCGCTGGACTTTTTAGAGCACTAGAAGTTTCCTTTGGTAATTACCGCGCTTGAACCTTCTACATTACATTATTAGGTTGAAAGGATACTACTGAAAAGATCTTTAAGAAAGCTAAATGGCAAGACATTAGGGATGATGAAAAATTCCGTAAGGCCTTTGGTTTAAGTCCAAAAGCCACCGAAAAAGATGTTGGTAAAGTAACCAATCCAGGTTATGGTTATCAAGGGATTTACATAAAGGATTCCCTGCGTGATGGAATTGCTAAGTACTCTGATTCGACGGTGTCTGAACCTAAAAACGTTAAGGTTTCTTTACCAGGTACTGTTGGTGATAATGAAGAGGGTAAGATCTGAATTGCTTCCCATAACTTTAGACAAAACCACGAATGGGGTGCTGGTGAAAAATTTAAATATTCAGCTTATCGTTTTAAGTTTGATGTAACAGTGGATTATGATGTAGAGGTAAGTGCTAAATGATGAACTTGAGCCTTTCGGGGTAGTATTCCAGGATATTGGAGGGGTAAATTCAAGGTTACCTATTCCTTTGATGGTGTCGTACCTTCTTGGAAGTATGGTCACATCCAAGTGCGTACGCCACAATACAGCTTTAATAAGCAGGAACAAAAGATTCTGTTTGTACCCCATGCTATCCAAAAGATTGCTGCTGAAGGTAGCAACTTAGATTTAATTAATCCATTCTTAAAGGATCAAAAATTAGATGAATTTGAGCACTACCACCCAGATTTAACTAAACCATTAGATTTAGTAGCTTATCTGTTATACGCAATAACCACGAGATCATAGTTGCCTAGTGGGATAATTAATAGAAATAAGAGCTTCTAGGCTTTTTTCATTTATTACAGCTTTTTCTTGGTTGATAAATTTACAAACAACTGCATTTGCCCATTTTACAAAAGTGCTAAATCAAGGGCTTTATTTGAAAATTGACCCGTTAAAAAGTTAATTAATGGGAGGATAAGCAAGCTTTAAAAAGCGCAAAATAGCGTTTAAAAAACACCTACTGTTTTCAGTTTTATTTTGAAAAATTCTAAATAAGAGATATAATTTTACTGATAGCTTTTCTTAAAAAATAACTTTTTGAGAGGGCGAAAATTCTTTTTACTAATATGTTTAATTTAAGTGATTTTAGTGAATTACCACGTTGGATTGGTGCCGAATTTTTAGGCACATTCTTTTTAATTCTTTTAGGTAACGGTGCTGGTTCGCAGCTAACACTCAATAAGATGTTTGTTAAAGAATCGAAAGCAAAGTTACTAACTGCTGCTTTTGCTTGAGGTATTGCTGTATTAGTTGGTGTTTTAATAGCTAACTCTTTGTTTGAGGGTGCGGGTAATATTAACCCGGCAGTGAGTTTGTTCTATGCTGTTTCGGGAACTATTCAAAAAGCTTTATATCCATTGCATGTAAACTTCAGCATTCCATTACTTTGAGTAGCACTCCTACTTGCTTGAGTAGCACAGTTTGCTGGGGCAATGTTGGCACAAGCATTGTTAAACTTCTTGTTCTGAAAGCATATTGAACAAACTGATCCACAATCGGTTTTGGTTACCCATTGTACTAACCCAGCAATCTTTAACATTCCTCGCAACTTTGCAACTGAGTTTGTAGCAACTAGCGTTTTAATTGCTAGTTTACTGGTGGCTGGTAGTTTTGGTGCCAACCGATTTGACCAGTCACCACGAGGGGTTGTACCAATGTTAGTGGTTACTGGCTTAATTATGTCTTTTGGTGCCGCTACTGGTACTGCAATTAACCCAGCCCGTGATTTAGGTCCGAGAATTGTTTACTGATTGAGTCCAATTAAAAATAAGGATCCTAACTTAAAGTACAGTTGGATTCCGGTAGCTGCACCTCTAAGTGCTAGTGTTATCTTAGGTGTGCTTGTGGCTGTTATCGTTTAGTTCCTTAAAGCGTTCTTTAAACTGCTTGGTCTTAACTGTAACTGGCTTACCCTGCACGTGGTGGTGCAACTTACAACGTGGTTCATAAGCTTCAATATCACCAATTAAAACGGTTTCGTTGCTCTTGGACACTATGCGCTGGGTGCGTTGGGCTAACTGACCACAGACATTACAAACGGCATCTAACTTACAAATCTTGTCGGCAATCGCTAACAGTTGGGGAATGCTACCAAAGGGTTCAGCGCGGAAGTCCGTGTCTAAACCAGAAACAATGACGTTAATGCCGAGATCATTGAGTGATTTAACCACTTCGACAATTTCACTCGAAAAGAATTGGGCTTCATCAATGGCAACCACATCAAAACGATCTTTAGTGAGGTAGTCATAGATTTCTAAAGGTGAATTAATTTCAATGGCTTCATCACTGTGACCGTTACGGGATTTCACGAGGTGTTGTTGGCGCGTATCAATCTTTGGTTTAAAGATAATAACGGGAATCTTGGCGAGCTTTCAGCGCTTAATTTTGCGCAATAATTCTTCGGTTTTACCGGAAAACATCGGCCCACAAATCACTTCGATTCAACCACGTGGGGATTGGTGGAAAACTTGGGAAAAGCTCATAGTTGCCCAATTATAATTAAACAAAGATGAGTGTGTTGCAAAAACCGCGCGGGGTTAAAGACTGGTATGGTGAGGAGTTAATCTACTTCAATTGAACGGTGCACCAGATCACTAATTTGGCCTGAAAATGAGGCTTTAGTGAGGTCAAAACACCCCTGTTGGAATACGCTGAAGCCTTTAAGCGCACCAATGCCAACGCCGATATTGTCAAGAAGGAGTTGTATGAATTCCATGACAAATCGAACCGGCTGTTAGCCCTGCGTCCCGAGGCTACTGCTGGGATAGTGCGCTTGGTCTGTGAGAACAAACTACTCCAACCACAAAACTACCCGTTAAGGTTGTTTACCATTGGCACCATGTACCGGTATGAACGCCCCCAAAGTAACCGTTACCGCGAGCACTACCAGTTTAGTTGTGAAGTGATAGGTGACACTAATCCAACCGTGTTACTCGACACTTTACTGTTGGGTCATGCCATTATCCAGCAATTGGGAATTGAAGGAGTGATCCTCAAACTTAACAACTTAGGTAACAGTGCTACCATTCAACAATGAAACCAAGCGCTGCAAGCTTACTTAACCCAGTTTAAGGCGCAGTTAACCGAGTTATCACAAAGCCGTTTATCGACCAATCCTCTAAGGATCTTGGACGACAAGGTTGATGGTCAACTACCCTTTATCAGTGATGCTCCCCAAATTGAGCAGTTCTTGGATGCTGAGCAGCAGGCACTGAATACTTGACTGCAGCAACAGTTAACCCAACAACAGGTGCCGTTTGAGTGGAATCCAACCCTAGTCCGGGGCTTGGACTACTATACTGGGGTAGTGTTTGAATTTGTTAAGGATGACACCACGGTACTAGCTGGTGGGGTGTATGATAACCTAGTGGAAGAGTTAGGTGGTACACCTACTAAAGCGCTGGGTTTTGCTTGTGGCATTGAACGCTCAATTAATTGTTTAAGTGCGGTTAAAAAGCAAGCAATTTTGGCCAATCAACCCCCACGATTACTGGTTATAGGTCTCACCGAAGCAGCGTTGGAAAAGCTGTTACAACTTTCATTGGGTTGAAGGGCGTATCATCCTGTGACTATTTATCCTAAAGTTATTAGAATTATCAATGGCATCAGAGCCGCGCAACGGTTAGGTTACCGCTTTTTAGGGGTCATTGGTGGTAATAATTTAGAACAGCAAACCATTACCGTTAAAGACCTGGCTACTGAACAGCAAACAACATACACTTGAGATGAATTTAGACAACGCCAAGTGCTTTAAGCAACGGGTTTTTATTGGCAATTTAACTTCCGAGCACCTCAATAAAACCGTTACTATTGCCGGATGGGTCAAACGGATTAAGAAGTTAGGTGAACTTAACTTTGTTATTGTCGGGGACAAGACTAATACTATTCAGGTTACCTGTAAAAATAAGGAACAGGTCAAGTACTTAACCAGAGAAGACTTGGTGATTGTCAAGGGCAAACTGAAACGCTTAGACAGTGTTCGGTTTGAAATTACCAATCCTACTATTACCTTATTTGCCAAGTCCAAAACACCCCCTCTTATCATTGAGGACAAAACTGATGCTTTAGAAGAAGTGCGTTTGCGTTACCGCTACTTAGACTTACGCCGTCCGGTGATGCAAAAGCGCTTGGCGTTACGCCATAAGGTAACTTTAGCCGTCCGGAACTGACTAGATCAAATGGGGTTTATTGAAGTAGAAACACCTACGCTCACGAAGTCCACACCGGAGGGTGCTAGGGACTTTTTGGTACCGGCCAGAATTAGGGAACATAGCTTTTACGCTTTGCCCCAAAGTCCACAAATTTATAAACAGTTATTAATGGTTGGTGGTACGGAAAAGTACTTCCAAATTGCCCATGTTTACCGCGATGAAGACAGCCGCAAAGACCGTCAACCTGAACACACCCAAATTGACTTAGAGGTGGCCTTTTACACCAAGGAAATGGTGATGGATTTAATCCAAAGGTTATTTGTCGATGTCTTTCGCCAAGTGTTAAACATTAAGCTCAAAAAACCGTTTCCAGTGTTAAAGTTTGCTGAAGCCTTTAACCGTTTTGGCAGTGACAAACCGGATTTACGTTATGGTTTCGAACTGGAAGACTGCACTGATTTATTCCAAGACAGTCCGAACCAATTCACTAACTTAATTAATGCTGGCGGCATAGTCGGTGGGATCCAATTACCGAATCTTTATTTAGACGAAGTTTCTTTTAAAGCATTGAGGAAACTAGCTAAGGACAACGGGGTTTCCTTAGAGTTTTACTCTGATAAAGCTAGTTCCCTAAAACAACCCCTGGATTTACCGTTAGCAGGCACCATCTTACTGGTAGCACACAAAAGTAAAACGCAAGCTTGAACGGCATTAGGCGCCATTCGCAATGAGTTGAAGTACCACCTCAACTTAGTGAAACCCAACCAGTACAGCTTTTGTTGAATTGTAGACTTTCCTTTATACGAGTTTGACGAAAAGGAACAGAAGTGGGTTTCGGCGCACAACATGTTCTCCAATCCCCAACCGCAGTGGTTGGTTAACTTTGAAAACCACAAGGCTGAGGCGTTGAGTGAACAGTACGACCTAGTTTTAAATGGGTTTGAACTGGGTAGTGGTTCAATTCGGATCCATGACCCAGAAGTACAAACCCGTTTAATGCAATCTTTAGGGGTGGATCCCCAACAGTTTGGCTTTGTGATGGAGGCTTACCAGTACGGTGCTCCTGTACACGCTGGCATGGGTTTGGGATTAGACCGCTTAATGATGATCATTAACAATGTGGACAACATTAGGGAAGTGATGGCCTTTCCGAAAAATGCCCAAGGGATAGAGATGCACACTAATGCCCCTGATCAGGTTGACATTAAAGACATAACAACAATATGGTCCAAACACCCAGTGAAATAAATACCCACCTTAAACACCTCTTGGCCTGTGATGCTTATAAGCTATCACACCGCTTAATGTATCCTAATGACACGACCAACTTATACAGTTGTTTAACAGCCCGGGGTGGTCGTGGTGGTTTTCCAAATTTTGTGTGAAACCACGAGTTTGCCAAAAAGATTATTCTTGAAGTGTTTGGTAACTTTTGTGATAGTGTGTTAGCAGTCCAAAACGATCCTGGGTTGGCCCAAGCGCTCACTGACAAGGTGACCACTGTTTTTGGTGATCCCCAGTTTGGGTTGGAGTTTACCCAGCACATCTGTTATTTAGCTAACTTTTTAAAACAACACCACCAGTTACCCTTAACGGTAAAGATTCACCAGAGTTCTGAGGGTTTAGCCTTCCGTACACCCTTAGTAACTATTACAGGTTCGGACCAAATGGTTCCCGAGTTAGTTTGGTTAGTCAACTATTTTGAAACCGTATTATTGGAAAACATCTGATTGTACCAAACTACTTTAACTGTCGCCCAAAGCTTAAAGCTGCTGTTGGAACGCTATGCTAACGAAACCGCGGATAATACTGAATTTACCCACTTTCAGTGTCATGATTTTAGTATGCGTGGTATGAGTAGTCTTCAAAGTGCTTTATATGTAGCTAATGCTCACCTGCAGTACTTTAGTGGCAGTGACACCATTTTAGGCGGTGTAGCTGCTAAATCGATCTTAGCTAGTGAACACTCGGTGATGTGTGCTGATGGTCAAGAGGGTGAATTGAATACCTTTAAACGTTTATTGGAGCAGTTTCCTAACAAAAATCTGTCCTTAGTGATTGATTCTTATGACATGTGGCATGTTCTAGATAACATCTTACCCCAACTCAAGGACTTAGTTTTACAACGACAGGAGAAGCTGTACCTCCGACCTGACTCGGGCAACTTTGAAACCCTTATTTGTCAGGGCAAACGGTTTAACCCCGAGGACAAAACTACCTGGGGAGTAATTGATTACTTAGATTACCACTTTGGTTCCACTGTTAACCAAAAAGGTTATAAGGTTTTGAACCAAAAGTTGGGTATTGTTTATGGCGATGGCATTACCTATGAGCGGATTGAGTACATTTTGGAACAGCTCAAACAACGTGGGTTTTGTTCTTCCAACATTGTCTTTGGGGTTGGTAGTACAACTTACCAAAACCTTAACCGTGATACGTTGGGATTTGTGTATAAGTTGACAGCCATCAAAAAGGGTAATACCTGACATGATGTTACCAAGAGTCCCATAACCGATCCCACCAAGCAGTCCATAGGGGGACGATTTGATAACCCCAACCTAATTCAGGTTTACGGTTAATGCACCTCAAAAAACCGTTAGTGGTTACTAAGGATTGCTTTTATTGACTTAGTTTCGGATCGCTAAAATGATTGTTAGCACCAATCTTTTTAAAATTTTCCAAACATTAAATTTTCTTTGTTGGTTGTAGTTGTTAGGTTGTTTAGTCAAAGATCCACCTTTTCGTAATAGTTAATTAAGTAATTTAGTTAGTTTCTTTTTATCCAATTTTAAACAGACTAAAAAAATCCGCTTTCGGTATAAAGCGTTTCTACTAACTTTATTTGCGTCAACCACAACGCTTACTGGATTCATAATACCTACTTTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCCGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGCCTTTGCTAAACAAAAGGATTGAAACCAGAACTTTGTTAAATTTCAAAAGCAGTTTGAAAACAAGCTTTTAGACCCAAAGAACTTTAGCTTAACCGATGTTTATAACCTCTTTAGTGGTTTTCAACAAAGTGTTACGGCTACTGTTCAGCTAATGAACGAGTTGCAAACCAAAGTCAATGAGGCTAATAATATTTTCCCAGTTGAAGCCTTCAAGGTACCTAAGGTTCCAGAAAAACTGTTTGGTTTTGTCAACCAAGGCTTCTTTCCTAAACTAAACCCTAAGGGCTTAAACATTGCTGATAACGTGGCTTCCTTATTTGAACAATACAGCTTAAAGCAAGCAAGTCTTAAGGACTTTGACATATTATTGGAAAAGAAAAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTAACTTTGAAACCACTTATGTTGGTACTGGTGGGGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACTACTAACTTTAGTAGCTTAGAAGAACTCCAAGCTAGCTTTAGTAAAACAGGCGATAACCTTACTGCCCAATTGTTCTGAAAACCAACTGTAACTAAGTTAGTTTCTGGGGAAAATGATCTAACCCATATTGCCCAAACAGCAATCGGTGAAAGTCTGTTTGACAGCCGGGTTGACTTAAGTGCTTCTATTATTAATAGTGAAGCAACCCTTAAAACAGCCGAAGCAACATTTACAACCCAAGTTTTAAATCCGTTTAAAGCGAAACGGGAAAAGGCTTTGGCCATTAAGAAAGCTGAAGAAGAGAAAATTAAAAAAGAACTTGAAGAGCAAAAGAAGCGTCAAGAAGAATTAGCAAAACAACAACGAGACAAAGAGGCATTACAAAAGTCACTTTGAAAATTTCAAGAATTTATTAGCTACTGAAACGGTCAAGGAAAAGACGTTAAGCAAAAAGAACAATTTATTCAAGCATTAGAAGCGGCGTTTTCTACCAATTGGAACGAGGTTTTCAATTTGTTAATTGCCGGTTTTAGAAGTGCAATTCAGACTTATTACAAAGATGGCAAAGCAGATCAAAGCCAAAACGCTAAAATTGCTTTTGGCGAAAAGGGTATTCAATTCCCTAAATCTGGCCCAGGACTTGACGGTATTTTCATGTCAGATTTCCTTCGTGGTAACCTTACCGGCAACGCTCACTTTGATTTAAAACTTAAAAAAGTTGAAGTTAAAAACACACAAGGAAAAGACGCACAAGGCAATGATAAAAAAGCCTCCATTAATTGACAAGCTAAGCAAAATAACTTCCCCTTCAGACAAGTAAACCCATGGGATTTTTCCTTTGAAGTAGAACTTAAGTATGAAGGTAGCTATGGCCTATATCCAGGTGCTAGGTTCCTTAACTTATTTGGTTCCTTAGGTATTCCTAACGATTGAAAAGGCGAAATGAGCGTTAAGTTTGTGCTCGATGGTAAGACACCTCAATGGATTGCTGATAAACCAGATTACCCAGGTTCCTTGTTTAAATTTGAAAAGAACCAGTTAAAGTTCACTCCCCACGTTAAGGAGCATGTACATGTTGAAAACAAACAATTTATGGAAAAGTTGAAAGAGTCAAAACTTGCATAACTTGGAGTTAGAAACTGGTGCTACAAAACCACCTGTTGTGGACTTAGCTAGTTATCTACACTACCTCATTTTGAACCACAAGTAGATTTAACGTATTGCTAAATTGGTTGTATAAGCCAAACTTTATACAATCTTTCAAACATTAAATTTTCTTTGTTGGTTGTAGTTGTTAGGTTGTTTAGTCGAAGATCCACCTTTTCGTAAAAGTTAATTAAGTCATTTAGTTAGTTTCTTTTTATCCAACTTGAAACAGACTAAAAAATGCGCTTTCGGTATAAGGCGTTTCTACTAACTTTATTTGCGTCAACCACAACGCTTACTGGATTCATAATACCTACTTTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCTGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGTGTTAGCGAAACAGAAAAACTGGGAGCAGAATTACACTAACTTTAAAAAGCAGTTTGAACACAAGCTTTTAGATCCGAAGAGCTTTAGCTTAACTGATGTGTATAACCTCTTTAGTGGTTTTCAACAAAGTGTAGCTGACACGGTTAAGCTAATGAATGAATTGCAAACTCAGGTAAATAAAGCTAACGACATTTACCCAGTGGAAAGCTTTCAAGTGCCTAAGGTACCACAAAAGCTATTTGGATTTGTCGACCAGGGCTTCTTTCCGAAGTTAAACCCGAAGGGCTTGAACATTGCTGATAACGTGGCTTACTTGTTTGAGAAGTACAGCCTAAAACAAGCAACCCTTAAAGACTTTGACATCGTTTTGGAAAAGAAGAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTCACTTTGAAACTACATACATAGGTAGCGGTGGCGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACCACTAACTTTAGTAGTTTAGAGGAATTGCAAGCTAGCTTTAGTAAGGTAGGTAATAATTTAACTGCTCAATTGTTCTGAAAACCAGTTGTAACCAAGCTCACTTCTGGAGAAAATGACTTAACTCACATTGCTCAAACCGCCGTTGGGGAAAGTTTATTTGACAGCAGGGTTGACTTAACATCCTCTATTATTAACAACGAAGCAGCCATTAAAACTACTCAGCAACAATTTGAAACAGAGGCTTTAGCACTTTTTAAAGCAGAACGTGAAAAAGCTTTAGCGGAATATAAAGCAGAGCAAGAACGAATTGCTAAAGAACTTGAAGAACAAAGAAAAGAATTAGAACGTCTCAAAAAAGAACAACAAAATAAACAAGAGTTAGTAGAGTCCTTATACAATGTTGCTAACTTTGTTAGTTATTGAGAAAAGCGTGGCAAGGACGTTACTGACAAAAAACAATTAATTCAAGCACTTAAATCAGCTTTTGCTACCAACTGAAACGAAGTGTTTCAATTATTAACTGCTGGAATGCGTGAAGGTATTAAGGAGTATTACAAACATAATAAACCTGACCAAAGCGCTAATGCTAAAAAAGCCTTTGGCCAAAATGGTCTTGCATTTCCGCGAACTGGCTTTGATGGTATCTACATGTCTGACTGGCTTCGTGGTGAATTGAGGAATAAGGGTAACATCAATCTCCATCTCAAACAAAACGAAACCACCGTTAAAAAGATTAGGGATGACATTAGTATCGAATGAAATGAAAGCAAGGGCGGTATTGAGTTTCACCAAACTTACCCTTACTGATTTGAATTTGAAGTTAACTTTAAGTACATTGGTGGTTACTCCTTAAACTGATGGGATGCCATTTGAGCTAAAGTAGCTGGGATCCCAGGTTCTTGAAAGGGTGAAATGAACTTAAAGCTAGTTATTGATGGTGAAATTCATAAGTGAATGGTAACCAAGCCAGACTATCCTGGAACCTTCTTTCCATTTGATGACCAGTTTGATAAGCTTTGATTCACCTTGCACGTTAGTCAAGAAATTAGTGTAAGGGATGAATCCTTCATGAATTTGCTCAAGAAACAAGGATTAGATAAATTAGATCTTCGAACAGGATCAACCAAACCACCAGTAGTCGATCTAGCTAGCTACCTCCACTACTTGATTTTGGCAGACAAGAGTTAATAACTAAATTAACTATCAAAAAGTTTAAGGGCATCTACCTAAATAGGTGTCCTTTTACTTTACCTTTCAAACTAAACAAAAAATACTCTGTTGGAGTAGATTTTTTCCTCTCTAACCCTTTTAAGTATTGGTTGTAAGTGGTAATAAACGCTTCGTGATGAAAATCAAAAGTTCATTAGTTTAGGTTAATAAATTTAAGTAAAAATTAAATATGCTTAATTGATTTAAAAAAATTTTTTTCTCTGATTGAGGTGGAGCAGAAGATAAGCAGAATAAAAATAAGAAAAATGAACAAGCTGTTTTTGTAGATAAAGTAATGAGTAGAACAGATTTAACTGATGAAGAAAAAACAAATTCTAGTTGATTACTATCAGATGTTAGATGACTATTTGGACGATGATTGAGACTAACTAAAACCACCCCGTGGGGTAGTTTTTAATTTTAAGAACTAACCTTGTCTTAGTCTAAACTAGCTCATTTTAGTGTTCTTTTAACAGCTTCGTTTCAACGCTTTGATTTCTTTTCCCGCACAGTTTGGTTCATTTGACTTTTGAACTGCTTAGCGATTCCAGTTAGTTTTTCTAGTTGTTTAGTGTCCTTTCAATAATTAACTGCTAAACCAGCTAGGAAGGCCGCACCCATGGCAGTGGTTTCCACATTCTTAGGAATAATCACCTCTAAGTTAGCAATATCAGCTTGAAATTGCATTAAATAGTTGGAATTGCAAGCACCGCCATCAGCCTTAATTTTTTTAATAGAACTGTTTAAATCACTAGCCATTGCCTTAATCAGGTCATTAGCTTGAAAGGCAATTGCTTCCAAGGTAGCTTTTACTAAATGTTCCCTTTTGGTATTAGCTTCTATCCCAAAGATGGCGCCTCTTGCTGAAGCATCCCAGTAAGGTGCGCCTAAACCAGTGAAGGCTGGGACAAACACCACTTCCTGTTCTTCCTTTTGTGCTAGCTTAGCATAAAAGTCACTTTCAGCTGCCGAGTACATTACCTTAAGACTATCCCTTAATCACTTTAAGGCTGCGCCAGCCACAAAAACACTACCTTCTAAGGCATACACCGGTTTTTGGTTTTCTAACTGCCAAGCTACAGTTGTTAATAAGTTATGTTGGGAGTATTTCAGTTCGTTACCGATGTTCATCAACATAAAACAACCTGTACCATAGGTGTTTTTAACCATCGCTGGTTCCACGCAGAGCTGACCAAACAAGGCTGCTTGTTGGTCACCGGCAACCCCACGAATAGGTACCATACCAGTAGCACTAGTAGATCAATGACTCGGTACTATGTCACCAAAGTGGGCATTGGATGGCATCACTTTGGGTAGGATTGATAAAGGTACTTTAAAGATGTCGCCTAACTCTTGTGATCATGTCATGGTGGTAATGTCAAACAAGAGCGTACGCGATGCATTACTTACATCAGTTACATGCATCTTACCACCCGTTAGTTTTCAAATTAACCAGGTATCAATTGTACCGGCTAACAACTTGTCTTCCTTAAGCATCTTTTGCGCTAAAGGAACATTTTCCAAGATTCAAGCAATCTTAGTGGCACTAAAATAAGGGTTAATTGGTAAACCAGTGTGTTTCTTGACAAACTCCTTTATCTTGGTGTCTTGGTTTAACTTATCACACAAACTAGCAGTGCGTTGATCTTGCCATACTATTGCATTGTAAACGGGCAAACCATTTTCTTTGTTTCATAACACAATGGTTTCCCGTTGGTTGGTAATCCCTACAGCAGCAATGTTGTTTGATTTAATTTGTGCCTTATTTTTAGCACTTTGCATCGTAGCTAATTGGACTGATCAAATTTCTAAGGGATATTGTTCGACCCAACCACTCTTAGGAAAGAACTGGTTAAATTCGTTTTGGGCAATCGCTACTTGGTTTAAATCCTTGTCAAACACAATCGTGCGGCAAGAACTGGTTCCTTCATCTATAGCAAGAATGTATTGTTGTTTTAGATCCATGGCAGATTGTACTTTCCGTTTCAGTTCGGCTTTTTAGTTAATTTCCAATTTAAAAGTCTGACAGCTTCCCGGGCAATGGCTGGTGCTGCAGTTAGCCCTGGGGATTTCATACCCCCTAAAATGACAAAGTCAGGGTCATTGTGAGCTATTCTAATAATAAAGTCATTAGTGGCTTTATCAATTGGACGCGAACCAGCAAAGCTTATTAAGGCATTATTAATGTTTAGACTTGGCACCATATGTTTGCCAATCTTAGTCAACATACATGGAGCATCTTTGGTAATGTAACGAGTAGCTTCCTTGTCTACCCCATCTAAAGCGGTGGGTCCGACTAAGAAGTTGCCATCTAACATGGGTGACACAATTACCCCTTTACCATGGATTGTTGGTACCATGAAAACCATTGAGTTTAAGTGGAGTTCGCCCTGATTAGTTACCACCACATACTGACCTCGACGGGTTGTTTGTTCAAAATCATCAGCTTTGGCTAAATGAGCTAAATAATCAGCATAATGACCTGCAGCATCAATAATCTTCTTAACTTTAAAACTAGGAGTAGTTTCGTTGTTAGTTCAAACTAAATAAGTCCCATCAACTTGCTTACTAATATTAGTAACCTCAGTATTAGTGCAAATTTGTACTTTGTTTTGCTGTGCTGCTAAAGCTAAACATTTGCTCGCTAACACTGGATCAATTGCTCAGCTACCCTCGACTTTTAATCCAGCCACAATTTCCGGATTAACGTAAGGCTCTAACTTTAAGGTTTCTTCTTTAGAGAGCATTTGAATATCTTCCAGATTAATTTGATTGGCAATGCCTCTTTGCTTTAGTACTTCTAATTGTTCGCGCTCCATTTCATTGAAAGCCACAATTAAAGTACGGATCTTTTGCCTTGGAAAACCTAAGCGTTTAAAGTAGGTATTTAATCAGAGCTTTTTACCCAAAATGTTGTATTTAGCAGTTAACTTGTGTGGATTGGGATCAATGCCAGTGTGGATAACACCACTGTTGGCATGGCTAGTCTCTTGTGCCAAATAATGGTGCTTTTCTACCAGTGTCACTTTGAGCTTGTACTGGCTCAATTCATAAGCAGTGGCACAGCCTATTACACCACCGCCAACTATTAAAACATCTCTTGTTTCCATAAAGGCTTAACTAATTAATAGTTACAGAAATGCCTTGACACGCCGATGCTTTTATCGTTTAAAGTTTTGCAGAGCTGAAAAAGTGGTTTTTTAAAAAACATTTAACTCTTATTTAACTACTTTGAAAATCAACGATCTAATAAGTTACTATTACGCATATTTTTTAGAAAGGGTGTAATTTTATGTTTGGAAAAGGGTTGGTCAAAAAAGGCCTTTTATTTTTTAGTGGCGTGAGTACTATGGCCGTCTTTTTAGTGTCTTGTGGTGCTACTAGAATTTGGGAATCTTCGATTCAACTGTTAGTTTCCAATGATGAAGCTACATTAGCTGACAAATCCTTTTCTGAGATGTCCTATGAAGGGATTAGAAGGTACTTTAGATCTCAAAAACATATTGAATTACCATCACCAAATAGTTCCCTGTTACAAGACGGCAACGGCCTTTGAAAACGTCCCGGACGTACTTTAAGTGATCGGATTGCTACCTTTAAAAACATTAAGAACGATGGTAGTGATGTGATTGTAGCTACTGGATTTAACCAACAAGAAGCACTACAAGCAATTAGCTCTGATGACCGTCGTTATTTAGCTGATAAAAACGACCTTGCTAAAGTTGGTTTCATTTTTGTCGATGGCCAAATTGAAAAGGAATATAACGTGATTAATAAAACGCCACAATTTAGATCAACCCCACTAAACATTTCATCCGTTGCCTTCCGCAGTGATGATGGTTCCTTCTTAACTGGAGTAGCAACTGCAGTTTATTTGAATTTAAATCAAGATTACTTTTTAAAGAAGAATGGAGCAACCAATAATAGTAGCCAAGATTTAACCGTTTCTGGTTTTGTCGGTGTTCCTATTCCTTCAACCCTTTCTTTTTTAAACGGTTTCCGTTTGGGCATTGCTTATTTTAATGAGGTGATTTACACCCATTTAAGTGATGCTGAAACAACATCTGATAACAAGAGTAATTCTTCAAGTGCTAGCAACTCTGTTTTAGTGCAACTTAAACAGATGCAAGGAAACGACAAAAAGATTAAAAAGATTAAATGGATATCTCCTAAACAAGGAAGCGATAATAATTCGAACCTCAGTATCGATGATCACAAGTCTGGTTCTTTCTCCAGCACTGAACCACGAGCAACAACTATCATTAATAATCTTTTAGACAAGGGTGTGAGTGCCATTATTCCTGTTGCCGGACCGCAAGTAAACTTAGCAGTTAACGAGGTGGCACGTCGCAAAGCGCACACTGCTATTATTGGTGTTGATAGTGCGCAAGAATTATTAGATATTAACCAAGATGCACCTGATAAAGATCAGTTAATCAAAGGTAATAAAAAAATTATTCCTTTCTCTTCTATTAAAGCCTTGGATGTTGCCATTGAAAACATGTTGATTGCCATTCAAAAAGGCAGTGATAATAATGGATATAAAGGATTTGGTTATAACAACATTGGTACGGTTGGAACTAGTTCAGTAGGTATTAGTGAGGCTGGTTATGAATTCTTAATTGATCCAGTTTTTTGAAAGACTACTCAGTCACAAGGGAAATCTATGGCCACAAATATGACTAATTTAAAACGTTTATCATCTGATGATACAAACACTAAGAAAGCATTAAAAGAGGTAAGCACCCATAAAAATGGTAGTGATAAAGATGGGATTATTGGTAAATATTCCAATTTATTAACTAAAAAAAGCACAACAGTAACTGCTGTTGCTCAAAAATCTATGACAGACAATAATAGTGGAACTGAGCAAAAAAAGAATCTTTCTGAAGTAGATACTAAGAAGAAAGAGAAAGAAAGTAAAGGTAAAACTCAAAGTAATGGTCAGGACAGTGGCCAACAAAACGGAAAAGAAACAAACGATATTATTTCTAAGTATTCTAAGCTGTTAACTATGACAACTATGAACAATAAAGTAATGTCATCTAAAAAACAAAGTAGCGATGATAACAGTTTTAAAAAGACTTCAGAAAACGGAGACTGAGTAATTAAAGGAGACGAGTTAACTAAGAAAAAATCAACTGAGCTTCCTGCTTTTGCTAAAGGTGCAGATTACCCTACTTTCCCTACAGAAGCGGTAAGCGTTATTAACGGAAGTACCGCTTTAGACGGCAAAGGTTTTAAGTGGAGCTTTAAACAAATTTAAAATAATACTCGGCTGATATGAAGAAGATTCAAGTAGTCGTTAAAGACCCTGTGGGCATTCACGCCCGTCCTGCTAGTATTATTGCTGGTGAGGCTAATAAATTTAAATCGGAATTAAAGCTAGTATCACCAAGCGGCGTTGAAGGTAACATCAAGTCGATCATAAACTTAATGTCTTTAGGCATTAAGCAAAACGACCACATTACCATCAAGGCAGAAGGAACTGATGAAGAAGAGGCACTAAATGCTATTAAAGCAGTTTTAGAAAAACACCAAGTTATTTAAGTAATTTAATTTAACTTAGAATAAAAGCAACAAGCCAATCCCCAAGGAAAAGCTTGTTGCTTTTTTATTAAGAAATATGCTTTACTACGACGACGAGGATGAATATGACACTTCTAATTCTGGTGGTGTCGTAGATTTAGCATTTTTAGCACTAATAGTAGTTTTTCTATTTACCGCTTTTATTTCTTGGTTTGGGGCATGAACTTGCTACAAAACAAGGCGTTTTTGAAAGGTAATTAATAAACCCATTAATGTAATAGCAACAGCTTTCTTTAATTTGGGGAACCTTACTTTTTCTTTGTAGCATTAACTTTTCTACTAGGAAATATACAAGCATTTACTCATGCTAATTAAGTAGTAGCACAGAACTTCAGGGAATAGCAATTAATATTGCCTTAATTGTTGGCATCATTAATTTACCTTTATTTATATTCTTCCGAATAATGCTGAAAAGTACTCATAATTTCTTAGACAAAAACATTCCACTACGAAAGAAATGAATTGAAGAAAAGATTGCTAGTGATGAATTGGAAGGATGAGAATACACCCAAATTTATCCTTTCCTAAGAAAGCTTTCTAGAAGAAAGCGTTACCATTTTCTAAGAGCCTTAGAAAATGGTGATAAACCAACTTCTGTTTTAACAGATAGAGAAAATCAATTGTCTGAAAATCAAATAAGTCAAGCACATGCTTAGTAAATAGACACTTAGCAAATTTATGTTTGCAAGTCTTTTTAATTTTTTAGCATCAAATTCGTCAATAAACTCTCTAATTTTTACCTAAAAATTCCTTATTTATTTCCTTATATTTTTATTTAAGAATAATCTTTTTTATTTCTCAAAATTTCCTTGATTTTATCCAAAATTTCCAATTTTATTTCTGGGAATTTTTACTTGTTTTATCTAAAAAATTACACCTTTCTTTTTTAGTGTTTCTCAAATATTTCTCTAAGAATGAGATTTTTATTCTTTGGTATTTCCACGTTATTTATCGAAATAATCTCTTTTTATTTCTAGAAAATTACTTTGATTTTTGTCTAAGGATTCTCAACTTAGTCCCTAAATTTTCTCCGCTTTTTTAGCGAAGAATATTTCAATTTATTCTTTGATTTTTATCTAAAAACTATCCCCTTAGTTACTTTAAATTTCTGTTAAATTTGTTTAGGGACTCTCATTTGTTTCTAAAAAACTTTTCCTTTTTCTTTCCAAAAGATTACAGTTTTTATTCTTAGAAACTTATCCGCCGTTTATCACAATATCTTTTTATCAAAACTTCTCCTTTTTATTCTTTAGGTTCCTCCTTTAATTTATCGAAATTTTCCATTTTTTCTTTTCTGGAATGTTTTTCTTTTTATAGCTAACTTAGTCTTTGAAATTTCTCTTTTTTATGTTCGAAGATTACGAATTGATAGACAAGAAATTGAAGATTTTTCAATTTTTCAAGCCTCCTTTTTCCAAAAAAACAGAATTGGTGAAGTTACTTTTTTAATTTTGTTCTAAAAGTAGCTTCGTTACCTTTCTAATTTAATGAAGATTAAATATTTCTTTATTCCCTTGTTTTCTTCAGTTATATTATTTAGTGCCTGTTCTTCTATTCAGTCTGATCTTAGGAATCTTATTAAAGAAACAACTGGCAAAGATTTTGACGTATCTAAATTAATTAAAACCTCTGAAGGAAGAAAAAACTTAATAAATTCTCTCAAAAAGTCTTACGAATCTAAGCCCGATGAAACAGCTAGCTTATTACTAATTGCTTGAAAGCAAAGTGCAGAAATGGGCGAAATAGGTTTTGATGATATTAAAGAAGGAGGAATTTATACCAGAGACAATGATCCTTTCAAATTGGAACAAAAGGTTGAGTACTTCAATATGGAATACAAAATTTTAGTGATGTTTTAATTAAAGCTAAATTGATCTATATCTTTAAATGGCATGGCGATCATTCTTCTTCAGAAGGCTTAACTGCTAAAAATGGCGGCAAGCATCATTTCGATCTTTTTCTTAATCTTAAATCCTCTTCAAAAAAACCATTTAATTCAAAAAGTTTTGATACAAAATCGGGAGAAGTTTTTCAAACGGAATGAATTAGTTTCCATACTTTAGTTAGTTGATCATTAAAAGGTAAAGATGAAAAAAGTCAAAAAAATCTTGACAAAATTATCATTAGCGAGTTTGTTACTAAAAAAGACCATGAACATGATTTTAATCTCTTTATATATTTAAACTATTCAATTAAGTAAGCTCTCTAGATTATTTAGTTTTGATTTGCTAAAAATTCAAGTACTTTACTTTCAGTTCAGTCTTTAAAGTTAGCTATTTTTTAGATCAAAGATTAAATTTATTGGATTTCTATCAAAAAATTCCTCTTTTTACTCTTTGAATTTTCTCTGTTTTTTATCCAAGAATTACACCCTGATTCCATTAAAAGTTTTTGATTTTTACCAAAAAATTTCATTTTTTATTTCTTAAATTTACCTTTCTTTTTACCTAGGCATTACCCCCTTATTAGCAGAAGTTTCTACTTGCTTTAACCAAAAGATTTCCTTTTTCTTCTTTAAAAAATTCGCTTTACTTCGCCTAATATTTACCCTTTTTTATTGAAAAAATTCCCTGATTTTAGCCAAAAGATAGCTTTTTCTTTGTCTAAACTTTTAGCTCTTTTTTTACCCAAAAATTTCCTTTTTGCTTTTTTCTCCAAAATTTCTCTTTTTTTGAATTAGAAGAATGCACTTTTTTATTCAGAAGTTTTCTGCCTTTTGTCTTTTAAATAATTTCTTAATTTTTATCGCAAAACTCTCTGTTTTGTTTCCTGATAAATTCTTAGTTTTTATCTAAAAGTTTCCACTTTCTCTTTTAAGAATTTCTCTACTTTTTATCAAGAAAGTTTCTTTCTCTTGTTTTTCTCTAAAAATTCCACTTCTTATTCCACTTATTTTCAGTTTTAATTTCTCCTTTTATTTTTGAAATAATTTCCTTCTTTTATCAAGTTCTTAACCTTGTTCTTATTTGTTAATTCCCCTTCTTTTAGCCTACAACTTCTCTTTTTTCTTGTTAAAAGATTCCCTTTCTTTTTATCAAGAAAATCTCTTTTTTTATGTCTTAAAAGTGGTCTTTATTTTGGTTCAAAATTTAGTCCAAAAACAGTCAAATTTTTAGCACAAATTTAACTGCAAAAACTGTTCAAAAAATCTCACAAAATTGCAATCATTTTTTTGCAAGAAAATTTATCCCAGTTTTAGGGGGCAAGCGGGGTCAAAAATTGCTACAATAAATACCGTTTTTTCTTTAAAAATTAAGTAGTTAAATTGAACGAACGTTTTTTAATTGAAATCGAAGGTTTAAACAAAACTTTCGATGATGGTTTTGTATCAGTACGTGATATTAACCTCAAAATTAAGAAGGGCGAATTTATTACGATTCTCGGTCCTTCTGGTTGTGGCAAAACAACTACTCTTAGGTTGTTAGCAGGTTTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTCTTGACATTAAAGACTTGCCCATTCATAAGCGTCCCTTTGCTACTGTCTTCCAAGACTATGCCTTGTTTTCACACTTAACTGTGTACAAGAACATTGCTTATGGCTTGAAGGCAATGTACACAAAGCTGGATCCGATTGATAAGCTAGTGGAACAATACCACCAAAGCTTACTGGACAAGCAACACCGCTTGCACAAGAGGATTGAACGCTTAGAAAAAAGTAATGCTAATCCACAACTGTTAGAACAACTTAAACAAACGGTTGTCGTTCAACAAAAACAGTTTAAACAACAAACAGAGACTTTTAAACAAAAGGAAAATGCTCGACGTGATGCCATTCAACAACGCTTGGTGCAACTTACCAAGGAATGGGAAAGCTTATCAAAGCAGAAGTTGCAACAATTAGAAGCGGAGAAAAAGTTGTTAGATAAAAAGTTTGAACAAACTGAGCGCAAATACCAAAAGGATGCTTGGATGGCAACCCACAGTGAAATGCGTTTAAAGCAGTTCAAACAAGAGGTGTTAGCTTTAAAACAACTAATTAAAACTAAGTTCAAACAAAATGAACCAGTTGACAAGTTGCAGTTAAAGCTGCAAACCTTAAAGCAAAAGTATGCTGCTAAAAGACAAATTGATAAGGAATACGATAAGTTGGTCTTAGCCTACAACAAGAAGGACTATTGAACTTCATACTGGGAAACCTATTCTTTACAACAACAAGAAGCCTTTGAAAAGCGTTACTTATCGCGCAAACTCACTAAACAAGAACAACACCAAAAGGTGTGTGCTGTAATTGAGTTAGTTGGTTTAAAGGGTAACGAGGACAAACTCCCAGAAGAATTGTCTGGGGGAATGAAGCAACGGGTAGCCTTAGCGCGTTCCTTGGTAATTGAACCAGACATTCTCTTATTGGATGAACCCTTGTCTGCTTTAGATGCTAAGGTACGTAAAAACTTGCAAAAGGAATTGCAAAAGATTCACCAACAAAGTGGTTTAACCTTTATCTTAGTTACTCACGACCAGGAAGAAGCCTTGGTCTTGTCCAACCGCATTGTGGTAATGAATGAGGGTAACATTCTCCAAGTTGGTAGTCCGGCTGATATTTACGATTCACCCAAAACCGAGTGGATTGCTAACTTCATTGGTCAAGCCAATATCTTTAAGGGTACTTACCTAGGTGACCTTAAGATTAAACTGCACAGCGGTGAAGTGATTAAAACTGATGTGGACAATAACTATGTGGTTGGTAAGGAATACAAAATCCTAATTCGTCCCGAAGACTTTGACATTGTGCCCAAAAACAAAGGCTTCTTTAATGTTCGGGTAATTGATAAAACCTACAAAGGATTACTCTGAAAGATTACTACTAAGTTAGTTGATGACACCATAGTAGATCTAGAGAGTGTCAACGACATTGAGGTTGACAAAACCTTTGGGGTCACCTTTGATCCGATTGATGTTCACCTAATGGAGGTATAGGATGAAACTATCGAAAAAATACCTCTTAGCTGTACCATTCTTTGTTTTAATGGTCATCTTCTTTGTAGTACCAATGGCATGGATTATTGTTAGTGGACTCCAAAATGAAAACGGTGCAAGTATAACGGAAAAGTACCAACCCTTAGTTGGTGGTTATTCTTTCTTTCAGAGTTTTTGAACTAGTCTGTGAACGGCGACTGTGACAGTACTAGTAGCCTTACTGGTTGCCTTTCCCTTTTGTTACTTTTTATCACAAAGTAAAAACAAGGTATTCCGCAGTTTTGTGATTGCCTTAGCTACTGCTCCCATATGGAGTAGTTTTCTCATTAAGTTAATTGGGTTAAAAACACTGTTGGACTTAGTTTTGGGGTTAGCGTTGAACCGGGTGGGTGATAATAACCTCACCTTTGGTAGTGGTTATACCTTGATTGGGATGATCTACCTGTTCACCCCCTTCATGTTTTTACCACTTTACAACAACTTTTGTATCTTACCGAAAAACTTAATCTTAGCTAGTCAAGATTTGGGTTACAACTGGATTACGAGTTTTATTAAGGTAGTTATTCCCTTTTCTAAAACTGCCATTCTGTCAGGGATTGCGTTAACTTTCTTTCCGTCTTTAACCTCGGTAGCGATTGCCCAGTTCTTGGACAACTCGAATCAAAATAACACCTTAGGTAACTATGTCTTTACTTTAGGTAACAATGGTTATGACAGTGCGATTGAGCGCGGCCGTGCTTCTGGTGCCATTATTATTGCGGCTTTAATTACCTTTGCCTTTTACTTTGTGGTGATCTTTGCTCCAAGAATAGTGCGCTTAATCCAGACTAAGTGTTTGGAATACAGGAGGGTTAATGTTTAAGATGAAAAGCTGTAAGTTATGACTACGGGGAAGTTTCTTTGTCATTGTCCTTGTTCTCATCTACCTCCCTTTAATAATAGTTGTCTTAGTTAGTTTTAACGGTTCTTCGACTAGGGGTAACATTGTCTTAGACTTTGGTAATGTTTTAAACCCTAACCCGGATGCCAAGTCAGCATATCTAAGATTAGGGGAAGCGGACTTTGCCATCCCTTTACTCAACTCTGTCATTATTGGCTTAATTACGGTCATTGTCTCGATCCCGATTGCCATCATGACTGCCTTTGCTTTATTGCGATCCAGGCAATGGCTCAATAAAACAGTCTTTGGGATTGCTAACTTTTCTCTAGCAACTCCCGATATTATTACCGGGATATCGCTAGTGTTGTTATTTGCCAACACCTGGTTGAGCTTTAACCAACAGTTAGGTTTCTTTACTATTATTAGTTCCCACATCTCCTTTTCGGTGCCTTATGCCTTGGTGTTAATTTACCCAAAGATGCAAAAGTTAAACCGTAACTTAATCCTAGCGAGTCAGGATTTGGGTTACTCACCCATTGCTACCTTCTTTCACATTACCTTACCTTACTTACTACCGAGTATCTTGTCAGCCATTCTAGTAGTGTTTGCCACGAGCTTTGATGACTATGTGATTACTTCCTTAGTTCAAGGTTCAGTTAAAACAGTCGCCAGTGAGCTCTATTCCTTTAGAAAGGGGATTAAGGCCTGAGCTATTGCCTTTGGTGCCATTTTAATCTTGGTCAGCATTCTAGCTGTCTTGCTAGTAACACTGCACAAGTACCTACGCTTTAAACATAAAGAAATGCTCCGTGTTAAACAATGAAAAAACAGTTAAAGTATTGGTTTAGTCTAATGGGACTAACCATGTCAGTTGGGTTAACTGCTTGTAGTTCCAACCAGTTTGTGTTTGCTAACTTTGAGTCGTATGTGTCGCCCTTACTTTTAGAAAGGGCAGAAGCCAAGCAACCAATGACCTTTCTTACTTATCCTACTAACGAAAAGTTAATTAACGGATTTGCTAACAATACCTATACGGTAGCAGTGGCCTCGAGTTATGCTGTGAGTGAACTGCAGCAACAAGGCCACTTACTACCAATTGATTGAGCTAAATTCAATTTAAAGAAAACCCAAAATGGTAGTAACCAAGCCACCATTCAAAACAAAGAGGATGCCAAAGAATTATTTACCAAAGAAATAGGCGACATTAGTGGTGAACTTTTAAACTGGGGTGTGCCCTACTTTTTACAAGACCTCGTGTTTGTTTACCGGGGCGAAAAGATTCAAGAACTTGAAGGTCAAGATGTTACTTGATCAACGATTATTAAAGCTATTGTTAATCACAAAGACCGCTTTAACAACAACCGTTTAGCCTTAATTGATGATGCTAGAACAATCTTTTCCTTAGCGAACGTCGTGCACCATGAGGTTAAGAATACCACGGTAGATGTTAATCCTACAGGTAGCACCTTAAACTACTTTGGCAACGTTTACGAAAGTTTTGCAAATTTAGGTCTAAAACGTGATAACCTCAACACGCTCTTTGTCAATTCGGACTCCAACATTATTATTAATGAATTAGCTAATGGCAGAAGACAAGGTGGCATTGTTTACAATGGTGATGCCGTATATGCCGCTTTGGGCGGTGATCTCCGCGATGAAATTAATGAGAATAACTTGCCCAACGGCGATAACTTCCACATTGTGCAACCGAAACACTCGCCTGTGGCCCTAGACTTTCTCATTATTAACCAGCAACAAACTCACTTTAGAGATGCTGCACACCAGTTAATTTACCAGTTGGCCTTAGAAGGCGCTGATCAAACAGCAGAAGAATTATTAAAAACTGATGAAGAGAAAGGTACCAGTGATGAGGACTATTACACTTATGGTGCAATGCAGAATTTTAGTTACGTTAACTATGTTTCGCCTTTAAAGAATATTTCTGATGAAACCACTGGGATTGTGTTTAAGGAAAACAAACAAGCTGATACTAAGCGAGTTGTCAAACAACAAAGTCAATCAGAACAACAATCTGAATCAGCAGAAAAGGAAGAAACGGAACAAGATGATTTCTACACTGCTACTCTCAAAAGTTTGTTGAAAGCGGACAGTTTAGATGACAAAGCTAAGAAGTTAGTAGACACAATTAAAAAGACTTACAAGATCAAAAAAGCCGACAATATTAATTGGGCTAACTTAATCGAGAAACCAATTACCCCATTACAAAGGAGTAATTTAACATTATCGTGGCTTGACTTTAAGCAACGCTTTTGATAATGGAACAACCTTTGTGTATCTTGGGAATTGAAACCACTTGTGATGACACTAGCATAGGGGTAATCACTGAGAGTAAGGTACAAGCACACATTGTTTTATCATCCGCCAAATTACACGCCCAAACTGGTGGCGTAGTGCCCGAAGTTGCAGCTAGGAGTCACGAACAAAACTTGCTTAAAGCACTGCAACAATCAGGGGTTGTTTTAGAACAAATTACTCACATTGCTTATGCCGCTAATCCGGGATTACCCGGTTGTCTGCATGTGGGAGCTACCTTTGCGCGCAGTTTAAGCTTCTTATTGGACAAACCCTTATTGCCCATCAACCACCTTTATGCCCACATCTTCTCGGCCTTAATTGACCAAGATATTAATCAGTTAAAACTCCCCGCTTTAGGCTTAGTGGTGTCCGGGGGGCACACTGCCATTTACCTAATTAAGTCCTTGTTTGACTTGGAGTTAATTGCTGAGACTAGCGATGATGCGATTGGCGAAGTTTATGATAAGGTGGGCCGCGCTATGGGTTTTCCTTATCCAGCTGGACCCCAGTTAGATAGTTTGTTTCAACCGGAGTTAGTTAAGTCACATTATTTCTTTCGTCCTTCCACGAAGTGAACGAAATTTTCCTACTCGGGGTTGAAGTCACAGTGTTTTACCAAGATTAAACAGTTAAGGGAAAGGAAAGGCTTTAATCCCCAAACCCACGATTGGAATGAGTTTGCTTCTAACTTCCAGGCCACCATTATTGATCACTACATCAATCATGTCAAGGATGCCATTCAGCAACACCAACCGCAAATGTTGCTGTTAGGTGGTGGGGTAAGTGCTAATAAATACTTAAGAGAACAGGTAACACAGTTACAGCTGCCTTACTTAATTGCGCCGCTCAAGTACACGAGCGATAACGGGGCGATGATTGGTTTTTATGCTAATCTTTTAATTAATGGCAAAAACAATTAAACATCCCCGATGGGGTAGATATGTAGCAGAAGCAGTTGGTCGTGGTCACCCCGATAAGATCTGTGACCAGATTGCGGACAGTATCTTAGATGAATGCATTAAGCAAAGTCCGACCAGTCACGTTGCTTGTGAGGTCTTTGCTTCCAAAAATCTGATTATGGTGGGCGGGGAAATCCTTACCACTGGTTATGTTGATGTGGTCCAAACCGGTTGAAAGGTATTAAATCGGTTGGGTTACACCGAAAATGACTTTAGCTTTTTGAGTTGTATTAACTCCCAGTCCAGTGAAATTAACCAAGCTGTGCAAAGTAATGACGAAATAGGCGCGGGCGACCAGGGCATTACCGTTGGTTATGCTTGTAGTGAAACCGAGCAATTAATGCCCTTAGGGAGTATCGTAGCGCAAGCGCTGGTGCAACGCGCTGCTCGTATCATTGACCAATACCCCTTCATTAAACACGACATGAAGAGTCAGGTAGTGCTCAACTATACTGGTAACAAAGTTCAATGTGAATCAGTTTTAATGTCGGTACAACATACTCAGGACGTAAGTTTAGACCAACTGCGCCAAACAATTATCAACCAAGTGATCCTCCCCGTATTAACGGAGTACGGTTTAAATGATCCTAAAATCAAACACCTAGTCAATCCCGGGGGTTCGTTTGTCGTTGGGGGCCCGATGGCCGATACGGGGTTAACCGGACGCAAGATTATTGTGGACACCTATGGTCCGTACGCTAATCATGGTGGGGGGAGTTTTAGTGGTAAAGACCCTACCAAGGTCGACCGCACTGGAGCTTACTTTGCGCGCTTTATTGCTAAACACATTGTTAGTTTGGGTTGAGCGGAAGAGTGTGAAGTGAGTATTAGCTGGGTCTTTTCCCAACCATTACCACAATCAATTCAGGTCAAATGCTTTAACATTAATAAGGAATTTAGTGAACAATTAATAAACCAAGTTATAAGTCAATATTTCAATTGATCTGTTGCTAAAATTATTGCAAAGTTAAAACTCCTTGATCAAGTTGAGTACTTTAGGTATGCTGTTTACGGTCACTTTGGTCACCAAACAGCCCCTTGAGAACAACTAAGTGAACGTGACTCGCTGCAATGTTTAATCAAAAATTTCCAATAAAAGCTCCTAAAGCACCATTTTTTAAAAAGAAAACACCACCAGTACCACCAAAGAATCCTGTGGTAACCAGCACTACCCCAGAACTCAAGAAAACCAAAATTAAAAAAGTAAAGGAAGCAGGGGATACCAATCTTTGAGATAAGATTAAAGCAAAGGTAAAAAAACACCCTTACCTGTTTGCTGTTTTAGTGTCCTTTATTCTTTTAATTGCACTTTTAGGCTGATTATTTGCAATTCCGATTTCGCAAATTCCTTAAGCTGCAAAAAGATAAAAAAGCAATGTTCGCTTAAAAACCTAAGCCACCAAGTCCACCAAAGCCACTAAAGGGATTTTTACCACCTTTCAGCATCCGGCCCATTTCTGCTGTTTTCGTTTGCAGTTTACTCCACTCCTTCATTAGCTTATTGAGTTCATCCATTTTACGTCCCGAACCCTTAATGATGCGTTGTTTTCGCGATGGGTCACGATTAATTAATTTGGGGTGCCGGCGTTCTTCACGCGTCATTGAGTTAATTAATACCTTTCACAGTTTAACCTTGGCTTCAATGCTCTCTACATGATCATCCGATACCGTTAAGTTACCCGGGAGCATCTTGACAATACTACTAACAGAACCCATTTGGTTCATCTGTTCCATGTAAAGCAACAGATCTTCCAGGTCCATCTTGCCCAAGAACATCTTGCTAACTGTTTTGGTTAAGCTCTGTTTGTCAAATACTTCTTCCACTTTTTCCACTAAGGTCATCACATCCCCTAAACCCAAGATCCGACTGGCAATCCGTTCTGGGTGAAATTGTTCTAGACCTGTGAGTTTTTCTGATGTCCCCATTAGTTTAATCGGAACTTCCAACAAACTCGTTAAGGACAAAGCCGCTCCAGCGCGGGCATCACTGTCAAGCTTGGTAATAATAAAACCGGTGAGTTTTAAACGGTTATGAAACTCCTTGGCAACATTAATAATTTCCTGGCCACTTAAACCATCGACCACCATTAGGATTTCATCGGGGTGGAGTTCGTTTTTAATTGCTACCAACTCATCCATTAATTCCTGATTAGTTTGTAACCGTCCCGCCGTGTCACAGATTATTGCTTGACAACCGCTTTCCTTAAAGGTCTTAACGGCCTCCTTGGTGGTTTGGTCGGGGGCTTGGGTACCCTTGGCAAAGAAGACAGACTCGGTTTGTTCGGCTAGGGTCGCTAACTGGTCAATCGCAGCGGGGCGGTAGATGTCTAGTGCCACTAACATCGCTTTTTGTTGAAACTGTTTTTTCAGTCACACCGCTAACTTCCCACAGGTAGTGGTTTTACCTGAACCCTGTAAACCGACTACCATTACCTTAAGTGGACGTTTACTAGTGATTTCTTGGTTGGGTTGGCTCAAAATGTTGATTAACTCTTGTTTAATTACTTTGAGCAGTCACTTCTGTAAGTCCTGACCGGGTTCTACGGTTTGACCAACGGTTTGTTCGCGAATCGCCTTAATGAAGTTTTTGACCACCAACAGGTTCACGTCAGCGTCTAAAAGAGCAATCCGAATTTCCTTTAAAACGGCTTGGACATCGGTTTCACTGATCGTTTGCGCGTTGATCTTTTTTTGCATCGAGCGCATAACGATGCTCGAGATCATTGACTTAAACATGGTGCTTTAATTATTTATTTTTTTATTATTTCTACGTATAGATAAAGCTAGTTTCATGACACCGCACATAAACGCAAAAAAAGATGACATTGCTAAGGTTGTTTTAATGCCCGGTGATCCCTTACGTGCTAAGTGGATTGCGGAACAATTTATGGAAAAGCCCCGGCTCGTCAACGAAGTGCGGGGCATGTTAGCTTTTACCGGTCAGTATAAGGGTAAGACCATTACCATTATGGGCCATGGCATGGGGATTCCCTCAATTGGGATTTATTCTTATGAACTGATGAAGTTCTATGAGGTTAATACCATTATCCGAATTGGTAGTTGCGGGGCGTTGCAAGATTCCTTAAACCTCCAGGACCTCATTATTGCCGCTAAGGCTTGGAGTGAGTCGATTTACGCTAACGACATGGGTGTGGAAGTGCCAGCAGACAAGATCTTAATGGCCTCCCCGCAACTAGTAGAGTTAGCTAAAAAGACCGCTAACCAACTTCAACTGGCCTTCCATGAGGGCTTGGTGTTCTGTGAGGATGCCTTCCACCAAACCAGGAAAGACGTGTTAAAGCTAGCACAAGAAAAACACGCTTTAGCAGTGGAAATGGAAGCGCATGCTCTGTACGCTAATGCCATGCTGCTCAACAAGCAAGCGTTAACGATGCTCACGGTGTCCGATTCTTTGGTAACCCATGCTGCTCTACCAGCGGAACAACGCCAAGCCACCTTTAAGAACATGGCTATCTTATCCTTGGAAATGGCTAGCCAACTCGTTTAAACAATGAAATTAGAATACAACCGCATTATTGACAGTACACTACTTAAAGCTGATACCTTACCCCACGAAATTGATGCTTTATGTGCTGATGCTCATAAGTACCAGTTTTACGCAGTTTGTGTCAATCCTAGCTATGTGCGGTATGCCAAAAATATCCTTAAGGGTACGGGGGTGAAACTGTGCACTGTCGTTGGTTTTCCCTTAGGACAAACAACACAACGCCAGAAGGTGTATGAAACTAAGATTGCCATTAAGGAAGGTGCGGATGAAATTGACATGGTAATGAACATTGCCGAGTTCAAAAAGCGCTGTGCCTGTGTCATTAGTGAAATAAGGGCAGTCAAAAAGGTTTGTGGTAAGCGAACGCTCAAAGTCATTATTGAAACGGCTTTGTTAAACCAGGACGAAATTCGTGATGCCGTTAATGTCTGTATTGATGGTAACGCTGACTTTGTCAAAACTTCTACCGGGTTTTCCATGCGTGGTGCATCATTGGAAGATATCACAATTATGCGCGAAGCTAGTGGTAATCTCATTAAGATCAAAGCATCGGGTGGGGTTCAAACAGCGCAGCAATTCCTTGACTTCTTTAATGCTGGAGTTAGTCGGATTGGCACTTCCAATGCGGTCAAGATTATGGAAGAATTGCACAAACTAGAAAGCCATGAACATCGTTAACCTTATTAGTAAAAAGCAACGCGGCAAGGCGTTAACGGAAACAGAAATTAACTGGTTTGTCCATTCCGTTAATAACAAAAGCTTGGCCGATTATCAGGTCAGTGCCTTTTTAATGGCAGTGTGATTTCAGGGGATGAATTCCAAGGAGTTGTTTTGTCTCACCAAAGCGATGGTGAAATCAGGAGAGAGCCTCCACTTTAACCACCACAGCAAGTTAAGTGTTGACAAACACTCCACTGGTGGTATTGGTGACAAGGTTTCCATTGCTTTAATTCCAATCTTAACTGCCTTAGATTACAGTGTCGCAAAACTGTCGGGTCGTGGTTTGGGCTACACTGGTGGCACAATTGACAAATTGGAAGCAGTGGGAGTAAAAACAGATTTTACACCAACAGAGGCGCAGAACTTACTAGACCAAAATGACTGCTTTATTATTGGTCAAAGTGAGGGCATTGCTCCCGTAGACAAGGTGTTGTATGCACTACGCGATACTACGGCAACTGTGGACAGTCTGCCCTTAATTGCTTCCAGTGTCATGTCGAAGAAGTTGGCAATTAACAACGACTACATCTTCATTGACCTTAAGTACGGTAAGGGCGCTTTCTGTAAAACGAAGACGATGGCTAAAGAGCTAGCGCAGTACATGTACAGTATTGCCAAGCAGTTTAAGCGCAAACTCTACATTAAACTAAGTGACATGAACCAGGTTTTGGGTAAAACGATTGGTAATGCGTTAGAGGTCTTGGAAGTAGTGCACTTCTTAAAACGGAATTGAACTGAAGTGGGTGCTGACTTTATTCAGTTGATGGAGCAAATAGTCACTGAAATCCTTATAGAAACAAAACGTGCACCAAACAAACGGGCAGCTGTAGCACTTTACCACGCTACATTAGAGGGCGAAAAACCATGGCAGCGTTTTTTAAAATTTATTGAGCTGCAAGGTAGTAGTCGGGAACGTTTTTTGGATTTAAAAGAGCTGTTTAATCCCCAGTATAAAGCGCCAGTGTTAGCATCTCAAAGTGGTACTTTGAGCTATACCAGTCCGGTGGATTTAGCCATGGTCTCGATTAGCTTAGGTGCTGGTCGAATGGTTAAAACAGATCTGATTGACCCAATGGCGGGGATTAAGTTAGTGAAACAAGCTAACGAAGTGGTAAAAGCTGGGGACACAGTCTTAGAACTTTACAGCTCCAAACCAATTACGCCTGCCCACATAGAAGCTGCGCAGCACACCATTATTATTAAGCAGTAAATAAACCTCAATGAAAGTTGATTTAGATTGAGTCCACCACAAGTTACAGGAGGTGGTCAATCATGCCTACACGCCTTTTTCAAAGTTTAAGGTAGCTTGTATGTTGGTGGCCAATAACCAGGCATTTTATGGAGTCAATATTGAAAACGCTTCTTACCCAGTAACCCTGTGTGCGGAACGTAGTGCGATTGCTAACATGGTCACTAGTATCGGCAAGGCCACAATTGATTATGTCTTTGTGTACTTTGACACTAAAACTCCAACCAATTCACCTTGTGGCATGTGTCGGCAAAACATCTTTGAGTTTGCCACTGATAAGACCCAGTTATTCTGCATTGAGAAGGACAAGAGCTTTAAACAATTTACTATTCCCGAAATTTTAAAGGGAGGTTTTCGATCATATGAACAGTAACGCATACTTGGAAGCTCAAAGGTGGCTAAGCCATCCGCGGGTAAAACCCAACTTAAAGGAGGTCATTACGGCTATGAGTGCTGAGGAAATCGAGCACTTCTTTAGCTTAAAGAAACCGAGTTTTGGCACTGCTGGGGTACGGGGCAAAATGGCACCGGGGTACCATGGGATGAATGTGTTTTCCTATGCTTACTTAACCCAGGGTTATGTCAACTACATCCAGTCGTTAAACCCAACTAAAAAACCATTACGCTTTTTAGTAGCCAGAGACACTAGAAAACACGGGGCATTGTTTAATGGCATTGTGTGTGATGTGATTACCTCCATGGGTCATGTGGTCTATATGTTTGATAACAATGAACCAACACCAACACCATTAGTATCTTATGTGATTAAGAAGTACCACTTTGATGGTGGGGTGAATGTCACTGCTAGTCATAATCCTAAAACCGATAATGGCTTTAAGATTTACGATGGTCATGGCGCGCAGTTACTAGACTTCCAAACTGATCAATTAATCGCAATGTTACCCCCAGTGGTAACAATGTTAGATTTCGAACCACGTGGTAACAACGAGCTGTTACACTTTTTGGATAACGAAGTGGTCTACAAAAACTACTTTGATGATCTTAAGGAAAGCCTAGTAGTGGACAACGACAGCTTTAAAAACCTCCCGGTGGTCTTTACCGGGTTACATGGTACTTCGGTGAAGTTGTTACCAAGGTTTTTAACCTATTTGGGTTACAGCAACATTATTAGTGTCCAACCACAAAACGTGTTTGACGCTAACTTTGCTAATGCTGACCACCTCAACCCCGAAAGTAAGGATACCTGAGAGTTAGCGCGACAGTATGCATCCAACACTAAGGCCAAGTTAATGATGGCAATTGATCCGGATGCTGATCGCTTTGCGATTGCCGAATGAAATCCGCAAACCCAAGACTGGCACTACTTCTCCGGGAACGAGTCAGGAGTTATGGTGGCTTACTATAAGCTCAAACACAAGCAGTTTAAGCGTCAACCTTACATTGTAACGACGGTAGTCTCTACTGATCTAGTGGATAAGATTGCCAAGAAGTACGGGGCTTTTGTCAAGCGCACAAATGTCGGCTTTAAGTTTATTGGTCAAGCTGTTGATCACTTCTCCAAAGACAATGAACTAGTAGTGGCCTTTGAAGAAGCGATTGGTATGATGGCTAGTGATGGTTTGAACCGCGAAAAGGATTCCTTTCAAGCAGCGGCCATCATGCTTGAAATTGCGCGCTACTGTCATAATAAAGGAATTAGCTTACTAGAGTTCTACCGTGGTGAGATCTTCGGTGAATTTGGTGATTACTATAACTGAACGGTACCACACACCATTCACGGAGTTAACTGGAAGGAAAAAATGGAACAGGTATTACACCAGTTAACCACAGCTACCATTAAAGAGGTTGTGGGACACAAGATTACCAAGATTAAAAACTATGTCGACATTAACCTAGTCGAATATGTCTTGGAAAACGGTAACTGGATTAAGTTCCGCATTTCCGGTACAGAACCGAAGTTAAAGCTGTACTTTAACCTATCTAAAGGTTACTTAGCAGCTTTAAAACACGAAGCGAAAAAGATGCACGAGTTCCTAGTGCGATTGCTCAACCTCGATAAAGCTTAGGTTTTCTAAAGGCAAGTTAATTAACTGCGTTTTGGTGGTTAATTCGACTTCCACTACCGCTTTGTTAATTGTTCCTTGGTATAGAGCCTTAATTTGACCTTGAATGTGAATCGCACCAGTTAAGTGGACTTCCACTAAGTTACCAACACTAAATTGTTGCAGTTGATCGACCTTAGTGTCACCTTCGACAATTTCGTATTTACCCAAGTAAGGAATTAACACTGATGGGTCAACCTTGTTAGCTTCACTTTTAACAATCGCTACATCCTCGTTTCATTCGTCTTGTTCGGCCTTATTAGCTTCCATTAGAGCTTGCGCAAACTGGTTAGTATCAATGATTTCACCAGATTCGTTAACTTGGGCAATTGCTTCGGGTTTAACTTGTTTGTGCTTGAGAATGTACTGAAACTTTTCGTCAAACTTGTCTGCATCCATTTCCACAATGGCAGTGTTGGTCACTAACACCCGCTTTTTCGGATTGACCGAAATACCCTTAAGCATTTGTTCGAGGTTATCAGCATCGGCTTGGTCTAAAGGAGTTGGTAACGCACCGCGACCAGAGGAACCAACAATTCCAGTAATACCAGGGAAGTTACGGATAATGCGTCATGCATCTTCACTGTAGATCATCTTAATGTAGATGTAACGACCTAGTAAATTCTTCTCGCTAATCTTCACCTTTAAATAACGGTCTTCATCTAACACAAATCACTTTGTAAAAGCGGTGTTCTTTAATGAACGGGGCAGCTTGCCGGATTTTAAGGAAAAGACTTCTTCGATGACTTCGCGTTCTTTCAAAACCCTTACATCTAAGATTTCGTTGTCAAATCCCAAAGCTTTTACCTTAGCTTTGAGGTTTCGCACAACTGCTTCATCCTTAACACTTACAGGGGCAACGTATCATTGCGGGATTAATTCCACTTGTTCCATAAACTAATTATTTACCTTTAAAAACTCCAAGCGCAATAAAGAGGTGGTTAATGCCAAAGTAGATCCCTATCATAATGGCACTAACTAAAACAATTAACAAAAAGATCGTAATTAATTTCTTGCTTGTTACCCAGACAATCTTGTTAACCTCTTTGTCAATCCCAAACCACAGGTTCGAAAACTTCTTGGTAAAAGGCACCTTAACCTTTGGGGTTTGGTCCACTTTTTTATCTGTGTCTTTAGTTTTACTGTCTTGTTTCTCTTTGGCCTTCTTGGCTTCTATTTTGGCCTTTAGTTGACGGTGTAACTCGTGGATCTTCTCATCGTTGTAGGCGGTGAGTTTTTCCTTGTTCTTTAAACCAAAAGGGAGTTTCTTTTTTTCCATGAAGCTTAAAAGGAATCGAGGTGCTTGGTTTTTTGGTTGCAGTGCTTGCAATACTTATTAATTATAAGACGATTAAGAACCTGCTTCGACCAAGACATTACATAGTTTCTACTTAAACAGTCTTGGCAAACAAAGATAATCTTCTTGCGCACGTGGAGTTGGGTCAATTATATATTATCGGTTTGGGTTACCAATTTAATCTTATGGTAACAGTAAAGTTGGAGCAAACCAAAGGGCAGAAAACGTGATCATTTCAAGGCTTTAGCAAACTTTTGCTCGACGTTTTCGGCAACCTCGTTAATAATGTTGGCTGCCATATTGGACATAACTAATTGGGCCACCATTAAAAGGATTTCTAACACCCCTAAAACAATTAAGACTGTGTTGAGGTTCCGAAAAATCGGATCACGACGTGGTGCACTCAATGAAGTTCTGTTAATGAGGTCATACATAATGCCAATGCCAATTAGGGAGATAGTGCACACAAAGAAGAAGATGCACACCAGCATCGTTAGTTCATTGAGCAGATTGAGCTTCTTAATCACTTTCATGGAATAAGGTGTAGAGCTTATTTGATTTTAATTGCAATCGTTTAGCTAAATAATGACAAGCATCCTTAACCTTAACGCCCATTTGGACTAATTGGGTAATTTCTTGTTTTAAGAGTTGGTCGGAATATTGCCCCACCGGTTGGGTGTGGTGGTTGTTAATCACAATCACAAACTCGCCCTTTAGAGTAATGTCTGGCAGCGGTGCACTTGTATTAAACCAGTAGTGGCTTTCGTGGAGCTTGGTCAGTTCCCGCCCTACAAAAACTTCAGTATTACTAAACACCGTTTGGATTACTTCCAGTGTTTGTTGTAAACGGTGTACCGCTTCAAATAAAACAATGGTGCCTTGGAAGTTTTGGTAGTGTTTGAGTGTTTGGGTTATTTGAGTGGGTTTGTGGTCCAAGAAGCCCAAGAACAGTAGTGGACTTTCGGGGAAGCCACTGGTTACCAAACCACAAACCAGCGCACTAGGACCATTAATTACTTCTATTGCGATTTGGGGTAGTTGCGTTCTGACATACTCCACCAATATGTGCCCGGGATCGGATACTGAGGGATAACCCGCATCACTGACCAAACCACAGTGATACTGGTGGATTAAGTTAGCTACCTTACTTAAGTTTTGCTTTTCCTTAAACTCGTTGTTAATAATGAACTGTTTATTTGAAAAGTCAATGCCGAGTAAGCCCAAGAGTTTTTTGGTTACTCTGGTGTCCTCACAAAAGAGCACTTCACACTTGGTTAAAGCCGCTTTAACTCGCGGACTAACTTCCTCAATGTTGCCAATGGGGGTGGCAATTACTTTAAGAGCTGGCATACTTGGTCAAAGGTAAGTTGGAGTAAATTAAGTTTGCGAAAGAGCTGCTTGTGGTTAAAGTAGCTTAAGTGTAGTTTGTTACACAACGCTAACCGTTTACTTTTACTGTTGAGGTTTAATTCCAAATATTGTTCCCAGCTTAAAGTGGGTTGGGTTGTTTTAGTGAACACCTGACGTTGTTGTAACGCTTGGATTAAAGCTGCATCAGTTGCTTCCGCTACCCCAATTTTAGTGCTGTTGGGTTTCATGTCTTTCCGAGCTATAAAACAACTTTCATACTTGTCTAAGTGTTGTTCTAACTGCCGGCGAATTTTCTGACCAGCAACATCGGGGTCCAAGAAGAGGATCACCGGTTGTTTTTTAGCTACAGCCTTAATCAGGTTAATGGTTCTTTGGTTTAAAGCTGAACCGTTCGTGGTAATAACACTAACATCAAACAGCTGTTGTAAGCGAGCTTGGTCAGTTTTGCCCTCACACACTATCACCCCATCTAGTTTTAGACGTGTTTTTTTATCCATTCAATGTGGCGCGTATAAATGGCAGAAATGGAAGAGTTATTAGCGTAACACAATAACACCTCTTCAAATAACGGTGCTAGATCAATTACCTGAAACTGGGGACACTGGTTAAACTTAGTTTGAGGAATGGAGTTGGAGACAAACAAATAGTCCACTAAGCCTTCGTTAAAGGCCTTTTGGAAGCGTTGCGGTGCTTCACCGTTAAACAAACCATGGGTGGCCATGACACAGACCTTTTTGGCATGGTGTTCCCGGAGTAGTTTAGCTGCTGCTATTACCGTACCACCGGTGTCAATCATGTCGTCCACTATTAGACAGTTTTTGTTCGCTACTTCACCCAAGACATTAATTGATTCCGCTACATTGTGGGCTGGGCGACGCTTGTCAATAATCGCTAGCGGTAGTTCCAAAGATGTTGCAATTAACCTTGCCCGCTTTACACCGCCATAATCGGGGGACACCACCACAAGGTCTTTTTTACCTAACAGCTCTACAGTGCGGGTTAAAAAGACGTGGTAGGTTTGCAATGTATCCACGGGTATATTAAAGAAACCCTGGGTTTGGTCGCTGTGGATGTCGGTTAAAGCTACGCGGCTCACCCCAGCCGTGGTTAACAAATCCGCTACTAATTTACTAGTTATCGGTTCGCGTCCCATTGTTTTGCGATCTTGCCGTGCATAACCATAGTACGGCAAAATGGCGGTAATACTTTTTGCACTACCCCGTTTTAAGGCATCAATTGCTATTAACAATTCCATTAAGGAATCGTTGACTGGCGCACAGGTAGACTGAAAGATAAAAACATCCTTATTGCGCACAGACTCCTCAAAGCGGATGTAGGTCTCCCCATCAGCAAACTCATTATGGGTAATTAACCCCATGGGCATTTTGAGTTTCTGACAAATACGACTAACTAAGTCGTGGGTTTTCGATAAGCTAAAAACAACGTGGTTATGGCGATCCATATTGTTGGCGCAATTCGCGCTGGATCGTTTTACTTTTGATGGCTTCACGTTTATCAAATTTTTTCTTAGGTTTGGCCAACCAGATTTCCATTTTAATTTTGCCATTTTTTAAAGAACACTTTAAGTGGAATAATGGAAAGTCCTTCTTGCTTTTGGCGTGCCGTAATTTGTTTAATCTCATTCCGGTTTAAAAGGAGCTTTTTCACCCGTTTGAAGGGGTGGTTGAACTCGCCTGAAAAGGCATAGGGCGAAATGCTAAAATCTTTAATATAGACCTCGTGCTTGTCAATAAAAACATAGGCTTCCTTTAAGCTACCTTGCCCTAAAGCTAGCGATTTGACTTCACTACCTTTTAAGACTAAGCCAGCACAATAACCCGTTAAAAGGTAATAGTCATATTGCGCTCTGGGATTGTTGACAAGTACTCGCATCTGTAGATGAAAATCTCTGTTATTATCTCCACTTACAACTGTGGTGCTTTAATTGTGAAAGCACTATGTTCATTAGTGTCAAACCAAACACCAGCATGTGAGTTAGAAGTGCTTGTGATTGATGATGGATCCATTGATAATACTAGGCAAATTATCAAAAAATTTCAAGCAAAAGTTAGCTTTACTTTAAAGTACTTTTACAAAAAAAACGGAAATTGGGGTAGTGTAATTAACTATGTCAAGGAAAACCGTTTGGCCAACGGCGATTGGATTACTGTTTTAGACAGTGATGATACCTTAAAACCTAATACACTCAACAAGTTGGCTAATTTAGTGGAAAAAGCGGATTATGACTTAGTAGTTTTTGACTACACTAAGTGCTGAAAAAAGATTAAACTGAAAATCCACACTTACCCGACTTGGTGAAAAAACATGACCCGGGAACTACAAAAGCAAACACCGTTTTGTATTCCTCTAGGTAAGTTTTTGAAACGCAACCTGTTTTACAAGTTGCCCAAGCTCAAAGAAAAGGTGAGTTTTCAGGATGCCTTGTATACGGCTAGTAGTTTAAAGCTCGCTAAAAAAGTGCGTCATGTTAATCAGTCTGGTGGCAACTACCACTTTAAGCGTGCTGGTAATTCAATGAGTATTCCCTGAAACATTAAGCGCTTTAGTGCTGAATTGGACATTTGTAAAGACTTAATTCGTTTAAATGCTCAGGAAATTGCCCTAGTGCACTTATTGCGACAACAGTTTCGGGTGCAACTTAAAGAAAAGCAGATACAACTAGCGGTTACCCGTGACTTTAACTTTAGTGGGTTCTCTTGGTACACCCGCTGCTTTTTATGGATGGTATACCAAACGATGTTAAAGCGCTACTTTTACTTACAAACAACTAAACAATAAAAAAGGCTGTGCAGCCAAACCACACAACCCTTTTTATTTTCTTTACTTTAACTATTTCAACAAGTCCGCGTAACGGTTCTTTTCCTTACTTGCCACCAAGAACTGTCACTGGTTTAACTTCCACTTTTTGGTCATTTCACTTACCAGTTCATCGCCATTCTTAAGATACGAAGATGGCAGTGTTACCACAATCGCAATGGCTGCTAACAACAAACCAGCAAACAGCGCCACACCAGCAATTGCCCCTGGGAGGATGCTTACTCCTTTCATTCCAGGGTTTGCTAGTTTCGGTGCTTCTAAGAACATTGAGTTAGTAATGTCAAAGGCGGTGTAGATCACATAACCAAAGCCCCAGATGTAACCAAACAACACGGACACTTCACTACGCTTGTACTCTTTGAATTCGTGCGGGAGGATCAAAATGGTACTTTGCATACTCCAAGCAAACGAACCACCAATAAAGGTTGCAATCGCCACAAAGGCAAAGCCAACAGCAGTACCCACACCTAGAGTGGCTGCAAACAATACGATTACAATCACCATTAAGGCATTCATGAAGAACATGAAGCTCAATCAACGCTTGCGATCGTAAACCGTTTTGTTAAATGGTGAAAACACCATGTAACCCATACCATAACCTAATACCCAGAGGATTGCTAAGGTTGGGAGACCGGCAAAGTAACCACCAGCACCCTCTAGTGGTTTTAATGTTTTAACACCATCTTTAACATTAAACGAACTCGCTGGACTCACTGCTTGGAGAATGTTTCATCAACCACCAGTAAGTGGGTTTACTAACACCACCAAACAGATGCCATACATCCCGATCATCTTTCAGGTCGCTTTTCGCTTTAAGATATCACCAGCACTTGGTTGGTTACCCAACTGCATTTGTTCCTTGGTTTGCTTTTGGGGCATAAACGGATCCATTTGCTTTTCAAACCAAGCAAACACACAGAGAATGCCAAAACCAATCAAGATTAAAGCAGTTAGGATAAAGACCCAGTACTTAGTACCAGCTTGTTGAACAGATTGGAATAAGAACGGTACAAAAGCAACTACAATCCCACTGTTAAAGCCCCAGAGGTTCGCGTTGGATAACACTGACTTACGACGTTGCGTTGAAAGTGAAGCAATTAACGGTTGGGAGTAAGTTACTAAAGTTGTACCACCAATAGCAATCGTGGAACGGAAAATAATAAACAACGCATAACCAGCAATTGATGAACTGGTACCAATTAAATTTTTATCTAAACCTTGAACTAAATCAATAGTTTGACCATCTGCTAACATGGTCTTGCCTTCTGCCATAACAGGCCCCAACAATTGACCTTCATGTAATTGCGAAGATAGACTAGAAAGTTTACCCATTACTTCCTTTGAAAGTGGTCGCAATAAAGTTAACTGGTTATTTCCCCCTAATGGATCACCAATAATTAAGAAGGGGAAACAGAGACACATTAGCCCCATCATAATCATGACCGCATAACGGTAACCAAACTTCAGGACCACTATTCCACACAATACGGATCCGACTGCACGTAGCAGGGTAATACTCCAGTTGGTGGCACTACCAGCAATTGATCCAGCACTGTTAGCAAAGAAGGAATTGACCCAACCATCATAGTTAGGTAAAACCTTAGTATTGCTATCTAAAATACCAGCTGGCTTACCAGCAATTCGGTCAATTACAAATCACTCTACGACAAACAACAAATAACCAAGCAAGACAATTGCCCACAACATGACAAGGCGTAAATCACTCGGCTTTTTTACAGACGTTTGCACAATCTCAACGACTCCTTAGTTAATTTATTTTTAAATTGTTTCAAGTCGACTTTTGTCTCTTATCTAAAAACAAGCTAGTTTTTTAGGCTTATCTTTGTTAACAAAATAAAAGACCAGAGCCGTTTGGCCCTGGACTTTTATTCCTTTTAGCGGTTTATCTAAGTTATTTTAAGAGATCCGCGTAACGGTTTTTAGCTTTATTCGCAATCACAAACTGTCACTGCGTTATTCTCCACTTCTTTGTCATTTCACTTACACATTCATCACCGTTTTTAATTCAGGATGATGGGAGGTAAATAATGACCCAGTTAATAACTAGAAGGAGTGATACAAACAAGACAATAGCGGCAATGGCACCTGGCAGAATATTGGCACTTGTATTACCAGTAGCAATTCTTGGTGCATCTAAAAAGACAGACGTAATAATGTCAAAGATGCTGTAGAAGACGTAAGCAAAGCCTCACACATAACCAAAGATAATAGAAACTTCGTTGCGCTTGTATTCCTTGTACTCGTAAGGGAGAATCAAGATCGAGCTACCTAAACTTCAACCAAAACCACCAGCAATAAAGGTAAAGATACCCACAAACGTAAAGCCAACCACAGTATTTAAACCAAGCGTTGCAGCAAAGATTACTATTAACAACACCAACACGGCGTTGGCCGTAAAGATAAAGTGGAGCCAACGCTTCTTGTCATAAATTGTTTTGTTAAACGGTGACAGTAAGAAGTAACCCAAGAGGTAACCCATTACCCAGAGAATTAATAAGATTGGACGGGCAGTTGGAATAATTGCTTTTTTAGCACCTGAAATAGCTAACAAGCTGTTAAACCAGGTTTGGGTTAACGGGTTAATTAAGAGAATTGTACCTACACCAGCTATTGCCAACAGCTTCCAAGTTGTTTTGCGTTTCAAAATTGATAACGCACTTGGTCTCACACTCAAGCTTTGATTGGTTTTTGATTGCTTTTGGGGCAACATGTGATCGACCTTCTTTTCAAATCAAGCAAATACTAACAACATCGCAAACACCAATAAGATCATGAACGTTACAACTCAAACCCAGTTAGCAGTCGCAAAGCGGCCAACACCTGGAATTAAGAAAGGTGCTGCCACAATTACAAGTCCTACGTTAAAACCTCAGAAGTTAGCATTCGATAAAATCGATTTCTTGCGGTTTGAAGAAAGGGAAGCAATAATTGGTTGCGCATAAGCAATTAAGGTCGTACCACCAATAGCAATCGTAGAACGGAAAATAATGAAAAACGCATAACCAGTCATTGACGCACTGGTACCAATTTCGTTGCCGTTAATTCCTTTAATTAAGCTAACAGGAGTGCCGTCAGCCAACATAGTTTGACTACCCACTTGAACAGGACCTAACAGTTGACCTTGTTGTAAACTGGAAGAAATTTTAGTAAGTTTACTTATTACTTCACTTGAAGCCGGTCGCAACAAGGTCAACTCGTTATGACCACCTAACGGACTACCAATAATTAAGAAAGGAAAACAAACACACATGAGTCCCATCATGATCATCACTGCATGACGGTAACCAAACTTCACCACCATTACCCCACATAAGATCGAACCAACTGCCCTCAATAAGGTAATAGTTCAGTTAGTAGCTTGACCTGGAATAAAACCAGCGTCCGCTACAAAAAAAGAACTGAACCAACCACCGTACTGGGGAACAGCACTAGTTTGTTCAGTTAAAAGGGAAGCCGCACTACCTCTAATGAAGTCAATCACAAACCATTCAATCACAAACAACACATAACCAAGTAAGACGAGACCTCACAGTGTAACGAGCCTCAGGTCGCTTGGTTTTTTTACAGAATTTTGCACTTCAGTAATAAAAGAAAACGCAAAAATTTTTATAAATTGTTTGCAACAGCAAATTCCGGAATTAAGCGAGCTCTTCTAAAAATTCCGTTTCTTTCTTGTTAACAAAAAGCGCAATAAAAAAGCAGACCACTGATGGTCTGCTTACAATTTATATTGAGAAATTTTTATGGCGGAAGCGGTGGGATTCGAACCCACGCACCGTAGAACGATCTAACACCTTAGCAGGGTGTCCTCTTAACCACTTGAGTACGCTCCCAGGAACTGGAATCTAAATTTATTATAAATATTGGATTTTCTGACTTTAGGATGTTCAAGCCATTGTTAAGCGCTGAGCTGTTTTTTAACTGAACAGCTAAAGATTTTAAAGATAAAACAAGCTTTTTAAAACAAGCGTGTCGTGTTTTACAGGCCAAAAACTGCATTAAAGAAGAACAAATAGCACTCACTGCTTTAAAAGAAAGGGAGGCACAAATCACCACCGGAATTATGTCAAAACTGGCATTGCCCCACATGCAAAGTGCTACCGTGTTAAAACCGTTTGTGGCGGTGTTTAAGGTCAACAATGTGGACTGGCAATCCTTAGACAACCAACCGGTTAAACTAATCTTTTTAATTGGCGTACCCAAAGATCAAGGCAACCTCCACTTGGAATTTATTTCGCAGTTTTCCAAGTTAATGTTGCAGGACGAGTTTGCCAACAAAGTGCCCAACATTCGCAGCTTTAACGGGTTGATCAATTTAATTGACAGTTTTCAACAAACTGCAGTTGCAAGTCAACCTGTTGTTAACGAAGCAGCAGCTCAGACTGAAGAGCCAAAGGACACAAACACTCAGTATGACTTTGTGGCGGTAACGGCGTGTCCCACCGGGATTGCTCACACCTTTATGGCCAAGGAAGCGCTGGAAAAGTTTGCCCGCGATCACAACCTCAAAGTTAAGGTCGAAACTCAAGGAACTGATGGGATTCAGAACCAGTTAACCGAGTCTGATTTAAACAATACCAAAGGCATTATTTTGGCCTGTGACCGCTTAATTGATTTAACGCGTTTTTACGGTCACGCAAATGTGGTGGAAGTGTCCACAACCAAAGCAATTAAAACACCACAAACAGTTTATGACCAAGTGGTAAAGAAGGAAGGCAAACTACTCGGTAATAAGTCCAGTGATAGTGCTAGTCAAACGGAACTCAAAGAAACCACTGAACAGCTCTCGTTTAAAGACTTTCACAAACGGATTTACCGCGCCATTTTAAGTGGGGTTTCTTACATGCTGCCATTTGTCGTGTTTGGTGGCATCTTAATTGCAATTGCCTTTTTAATTGACATTAATAATGCTGGCAATGCTGGTAAACAATTTGGCTCTAAAGATCCCATAGCTAACTGGTTTAAAACACTCGGTGGGTTGTCGTTTGGTTTAATTGTTCCCATTTTAAGTGCTTACATTGCCTTTGCTCTAGTCGGCCGGCAAGGGTTACTGCCCGGCTTTATTGTGGGGTTAATTTCAGCTGGTAAGTTTTTACTCAACATTGACATTGTTACTGGAAAAATTGATTGAGCGACTGAATCAAAAGTATCGAGTGGTTTCTTTGGTGCCATTTTTGGAGGCTTGTTGGCAGCAGTGTTAATTATTGTGCAGCAACGTTACATCTATAGAAAATTACCGCAAGCATTACAAGGCATTAAGAACATTCTCTTTATTCCGTTACTAGGAACGCTAGTCACAGCAGCCTTGTTCTGAGTCATTAACATTCCCCTAATTTACTTGAACTATGGTCTGTCCAAGTTCTTGCAAATTATGGACAAACCATATTTGGCTCCCTTACTTGGTTTAGTGATTGGACTCATGATGTGCTTTGACTTGGGTGGACCGGTCAACAAAGCGGCCTATGTGTTTGGGGTAGTTTCGCTAGAGAGCCAAAACAGTGGTACGGTTGCAATGGCTAGTGCTATCTTATCGGGGATGGTGCCACCATTGGGTATTGCCATTGCTGCGACCATTCGCAAGCAGTGCTTTGACAAGGAAGAATTACCAGCAGCGTATGCTTGTTATGTGATGGGATTAAGTTTTATTAGTGAAGGGGCGATTCCTTTTGTTGCTAAACGGCCTAAGATTATGTTAGCTGCTAACCTAATTGGTGGTGCTGTGTGTGGTGTTTTAACGGGAGCATTTGCGCTAACGATTCGTGCCCCTCATGGTGGCGTGTTTGTCTTTGCCTTATTGAAAACTAACTTAGAGGGCATTGCCGGCAACACACTCCAAATCGGCGCTGGGGTTGGCTTAGCACTTCTAGCGCTAATAGTATCCAGCTTCATTAGTGCTGGCATTATTATTGGTCATAACCTGCTTGTAGTGCGTAAAAAAACAAAACAACTAGTAAATACCAATGCTTAACCACAACAGTAAAGTTTGAATAGTTAACTATGCTTGTGCGATTGACTATTACTTAGATAAACACAAACAACAACGCGGTGTGTTAACACCCGGTGGTAAGGGAATTAACATGGCGATTGTAATGGCTTTATTTGGCATCAAACCAACCGTGTTAACCTTTTTGGGTCAACCTACTAAAGATTTATTCTTGCAGTTGTTAAAACCGTACCAACTGGACTTAGTTAGCTTTCCAGCGACTACCCAAACACGGATTAACGTGAAGCTGTTGGATGGTGCCCAAACAACTGAAATTAACGATGTCACCCCGTTAATGGAGGAACAAGCAGTCCATGAAATGATTGCTTACCTTAAGGCTAACGTTAAGCCCAATGACCTGTTGGTGTTGAACGGCCGTTTTTTACAACGCGATTTAGTTAAGTTGTTAGATGTTGCTTTTAGCTTAACCAAGTATGTTGTATTAGATGTTGATGAACCTCAACTATTACAACTGTTAAACCAGCGCCAACCCTGGTTAATGAAACCGAACCGTGATGAGTTTGTAGCAATGGTTAATGCAAATAACAGTAACGTTGACCAACAAGAGTTGGTGCAGTTAATAAAGCAGTTTCAGACTACACAGAACTTATTAATGTCAGACGGCGCGCAGGGTGCTTACTTCTTTGATCAACAGCAACTGTTATTTATGGAAGCGATTCCTCCCCAACAGTTAGTTTCCACTACGGGAGCGGGAGATACATTGCTGGGTGTTTTCCTAGCTAACTTACTGTTAGATAAGGATCCAGTGGGTAGTTTAAAGGTAGCGGTTAACTATGCTAGTGCTACGATTAGCAAGCTCGGTGTGGTCAACAGTAATGACCAAATAGTGCTAAAAGCTACTAATTATTACTATTTATAATTAGTAGATGTCAACTAAAGAACTAGCCAAACAGCCAAAGGTCAATCAAGAGAGGGAATCGATTTTGTTACCCGTTTTGCTAGCTGTTTTCAGTATGCTCTTCTTTATGCTACTGTTAGCTTTAGTGTTGTTGTACGCTATTCCAGGTAAAGTTAATAACTTGAACTAGCCACAGGTTTAACTGTGTTCAATTGGGAAGCCAAACTTAATCTCGCACTTTTAATGAACTAGTTTTTGTGCGCAACTTAATTAAAAACGTTTTTCGCTCTTTTCGCACCGCTAAGATTGCCTTAATTGCGCTTACCTTTTTAATCTTTGTCGCGGTTGGTAGTTTCGTGTTATTAAACAATACCGTTAATAACTTTAACGCTTCCTTTCAACACGTTACCGAACAAGGACAATTGTCTAACGCCATTATTAACGAGCGCTATGACTTTGGTAAACTCGAGTTCCAAAAAACGAGTAATGATGCTACTAGTTCTGCCCAACCTAGTGTTAGTACTCGAAGTACTTTAACTGCCCAAAAAGCAAATGGCGCTAATTCCAGTTTTACTCTGACCTTAACACCTGACTCACGGACTTCATTTATTAACAACGCACTGCAACTAAAACCGGATGTTTATAACAGTTTAGTTACCGTTACTTTTAGTTATGGCAGTGAATCTGATCAAACCAAGAAAACCCAAATAGAAGAACAGTCCAAGTTAATTGCCGCAAACAATTTAAGCCGGGCATTACAAACCGATAAGGAGTTATTAGTTACGGGACAGTTAGAACAGTTAAAGGCTACCTTTCGCACTTATAAGGCAATTAACATTACTGATCAAGGAGTGTTCAAGAAGCTCATAGCCTCTGAACCGACCGATCAAGTTAACCGTCTCGTTCTGTTTGACGGACATCAACTAGCACGTTCCCGCCAAGTCGAATTTAACGAGCTGGTTAGTCAGTTTACCCAGGTGCAAGCTAAGGGTAAAGATCAGTTATCCACTACTCTAAAGAATGGTAATTACGAACAACTTTTGCAAGCGATCGCTAACAACTTTGGTAGTAGTGAAACTGAGTTACTTAAAGAAATTCAAGATGCTTTAAAGCAAACGAATAGTTCCACCACCCTTGCCCAAACCCAAAGTAGTCAACAATTTAGTAAGTTAAAAAACCTGTTTGATTCTGGTAGTACCTTTACTAACATTGCCGGCAAACTCACGTTGCAGTGGATGGAAGGTACAAACAAAAAGCAACTGGTAATCTTTGACCCGAGTAGCTATGAAACGGTAGTAGCACCGGGTAACTGGAGTTACGAACAGCAACAGGGCAAAGCGGTGTATCAGGACATTAACCACTGAAACCGGATTAAGCGTTTACCCTTCAATGAGTTTGAACGCGAATTCCAAAAGATTGATAAACGGTACAAAATGTTGGTCGACAAAATTGAGTACCTCATCTTGGGAGTGGGAATTACACCAGACTTTGTCTACCCCGTCTTTAGTGAATCACTGTTAATTCCCAATACGGCTAACGAGCAGTTGTATTATGTCAACCAAACTGGTTATGAACGTACCGCTAGCTCCTTTTTAACTAATCCCACCGAAACTGGCATAGTAGCACGGTTACCTAACCTCAACCATGACCTTGATGTAATTAACCAGTGGGCTGTTAAAAACATGTCCTGACCCCCGAACCTCAAGGCCGCTTACAGTAATACCGATACCACTAACATCCTTAACCTGTTAGCAGCGCGAACCGTTTTTATTCCTAACCTCATTAACACGATTAGCTTAGTCTCTTCCTTCTTAACAATTGCCATCTTACTAGTGGCCATCTTTGTCACCATTTTAATCTTGGTTAGTTACCTCAAAAAGAACACGGAACAAATCGGCATCTTAAAGGCTAATGGGGTGAGTGGCCGGAAGATTAACTTAAGCCTGTTAATCTTTAGTTTAATCCCGGGGATAGTGGGGTCCTTTATTGGTTATACGTTAGGTATTAGTATGCAAAACGTGGCCATTAACCTCTTTAGTAACTACTGGTTTATCCCCACGCAAACTTCGAGTTTCTCGGTGTTAGGGTTACTCTTCTTTGCCTTATTAGTGGTTCTTATGATGAGTGGGATTAGTCTGTTTGTGGGGTGACTGATCTTAAGGCAGGATGTAGTCAAGATTCTTAAACACGACAGTGAGTTTAAGGTATCGCACTTAGGGTTGTGAGCTAAATGACTGTTTGCTAAGTTTGGTATTATGACCAGATTTCGTGTCGCTTTAGCCTTTAATGCGCCGTGGAAACTGGTCTTTTTAACCTTAATGAGTGCACTAACGATGATGATCCTAAACCTTAGTTTTGCCACTAAGGATGCCTTTCAGGTAGCACAAACAAAGACTACTTTAACCCAATCGAACCACCAGTACGAGTTTGAACTAGCATCGCCAACGGAGCAGAGTGGCTTGCTCAAGTGACAGCTGTTTGCCGAATTGGGCGCTACGGATGATCGTACTACCGATGCCGTTAAATTAGCAAACCGAAGGATGGAAATTCAAAGCGTTGACGAATCCAAAAAGTGAAAAAACCAACAGGTAATTAACTTTGTTACTGATGCCACCGGTTTTAGTAATGACATTAACTATTTAGAAAACATTGTTCAGTCCAAAATTGGCTTGGACTATTCACTGGGTTTTGGTAACGTTTCTTCCAATCCGTGGAAGCTGAGTGAAACATTAATGCCCGCCAATCAAGCATCCGCTTCCAACACGGCTTTCCAAAACTTTTTAAAGGCGATTATTGTAATTGACCCTAACGAGGGCGGTAAGTACATTAAACAAACTAAGGATGAAGTTACTGTGCGTTTTTTATACGAAATAGACAGTAGTAAAGCTTTAAAAAACGGCAACACCAAATCAGTTAATAAAATACAAAAAAGTACCGCAGCACCCCAAGCCCAACAAGGACAAAGTAACCAGTTAATGCTTGACAATGATTTTGCTAAATTCTTGTACAAGCAGTTTAACCTAATCAAGGAAGGGGGCAAAGCAGATCCGGTTGATCTCAATAACTTTGACTTTGCTAATCAGAATCAGATTAAGCGTTTTTACAGTAAATACAACGCCTTACCACCGGTAGATTACAAGCTGGCCTTTAACGTGATCGGTCTCCCTAAAGAAACAATTGCCAACCAAAAGGATGCGCCCCTGTTTGGCTTTTTAACCTTGCAAGCGCAACTGGGCAATGACGCGATTAAGATTAAGGGGATTAAAGACTGAAAGCTGGATAACACCAACTTGGGTCCTGTGTTGCGTACCAATGAGGACGAGATTATTAACCAGCGCTTGTTTAGTAAACCCAACGGTATTATTATCAACAGTTCAGCTGCTAAAAAGTACCGCTTAAAGGAAGGTAGTCAGCTCCATGTCAAAATTCACAACGGTTACAAACGAGTGAATGCTAAGTTAGTTAACCAAGATCCCACTTTAACTGCCACCTTTAACATTGTTGGCATTAACAATTCGGTGCACGAACCGGAGTTTTACACGAGTTACAAAACGGCAGCAACACTATTGCAATACCCCAATGAATGGATCGAAAAGCAGTTACCCTTTAACAGCTTTTATGCCAATTCGATCTTGCCCTTTACCCAGTCTACTTCCCTCTTTTCCGAATCTGGCTTATTCCCTGGTACCAGTAGCTTTGCCGCTAACAACACGGTGCTTACTGAAGTAATTAAGAAAACGATTGAAGGTGGTAAAACTAACAGTAAAACTGATGTGCAAAAGCGCGCTGCTCAAGCTACTGCTAACGGTAAGGATAACAGTAGTCAAAACAACAAGATGAATTATGAGCGCTTACAAAAGGCACTGGGCATTACCAGTGATTTAGATAGCTCTAAAGCCGGTGAATACGCCACCATCTTAGAGCGGGTGTATGATGGTTTGCCATACAACTCCACTATTAGCTTTATTAGTAATGTGCAGGCCAACGATGCCTTGTTTGCTAACATTGCTAACACCACCCAGCAAATCCAAACAGCGGCCATTGGCATTATTATTCCGATTATTATGCTAATTGTGTTGTTAGTATCAACTACCCTAATTCAGGAATTGAAGAAGATTGCGATTCGTTTAAAAGCATTGGGCTATTCCAACCCGAAGATCTTGCTGTCCTTCTTGTCGATTTACCTCCCGTTATTTATCTTTGGTTTGTTAATTTCGACCCCAATTTCGATTTATCTAATTGCACTCCACAATCAAGTTATTTTCTCCAGTGCTGCCATCTTACTGGAAGCCACATTAAACTTCCCCACGGTCATTCTGTCGATGTTAGTGTTAAGTGGGGTGTTATCAATTACCTTTGTGTTGAATTGGTTGGAGTTGAACAAAATCAAAATTGCCAAGGAGATTAAGAATAGCTAATGCGATACTTTGCCCTCACTGATTTAACCAAGCCCACTGCAAAATTTACCACTGATGCCATGGAACTGCGTCAGATATCCAATGCTTACCTCAACCAAGCCCAAGCATATTTGCAAAAGGGTTTAAAGCAACTCAAAAAGGATTACAAGAATGCCATTTTGTATACCCCCAAAACGGAGTACAAGCGCTTTTTGAAATGGAAGCAAACTTTTCTTCAAGATTTAAACCAAACCCAAAAGCGCTTCTTTATTGTTCGTGCCCAACATTTTTCTTACGTGTTGTTGTTTAACTTATTGGATGAACAGGTGGAAGCGGTGATTGCGACCTTTAACAACTTCTTGGATGAACACCGCTTGGAGGCACAATCCAAGCAGTTTGATTTAGACACAGCTGTCAATGAACTGCATGATTACTTTGACCAACTGCAAAAGAACACCGCTTACAGCGAGGATTTACCAACTCACCTCACGCAAAAAACCGAGCAATTAATTAACGCTCGTAATACCCAGTTAACGAATTTGTTAAACAAGATTGCTACTACCAAACCACCCCTAAACAAACAGCAACGTTTGTTAGCCAGTTTTCGCAATTACCACGAACACCTCTTTTTAAAGAACGAGGTCAAAAAGGTGACTTGGTTAAACGAACCACGCGCCAAAAAAGAGTCTGTTACCCCTGATGAAGAACACATTATTGAACTTAAAAACGTTTACAAGTACATTACTAATGGGGTGACTACCAATGCCGTTTTAAAGGGCATAGATCTCAAACTCAAAGCCCATGATTTCATTGTGATTTTAGGTCCCTCTGGCTCGGGGAAAACGACCCTGCTCAACATTATTTCCGGGATGGACCGGCCATCAAGCGGATCTGTTGTCGTTAATGGTCAGGAAATGATTTGCATGAACGACCGTCAGTTAACTAACTTTCGCCGCAACTATGTCGGTTACATCTTCCAGCAGTACGGGCTTTTACCCAACCTAACTGTAAGGGAAAACGTTGAAGTGGGTGCTAACTTGCAACGTAACCCTGATAAGCGGATTAACATTGATGAACTGTTAGAAGCAGTAGGGATGAAACACCTCCAAAAGAAACTCCCCAACGAGTTGAGTGGTGGACAACAACAACGGGTCTCGATTGCACGGGCGTTTGCTAAAAATCCCTTACTCATTTTTGGTGATGAACCAACTGGGGCTTTGGATTTGGAAATGACCCAAATTGTGCTCAAACAGTTTTTAGCCATTAAACAACGCTATAAAACCACGATGGTCATAGTGACGCACAATAACCTAATTGCCCAACTAGCCGATTTAGTCATTTATGTGGCAGATGGCAAAATTCAAGCACTGCAAGCAAACCCTAATCCCAAACAGGTTGAGGACATTAACTGAATTTAGATCTAATAAAATTAGAGCAAATGAAAAACCTGTTTGCTTGCCAACACTTAGCGTTAAGTGCTATTCAACACGCTAAGGGTGGTCATGTGGGAATGGCCTTAGGTGCTAGTCCTATTTTGTACACCTTATGGACTAAACACATCCAGTTTAACCCTAACTGTCCCAAATGGATTAACCGGGACCGTTTAGTGATGAGTGCAGGTCATGGTAGTATGGCCTTATACCCGATCTTACACTTTGCGGGGTTAATTACGAAGCAGGAAATGCTCCATCACAAATACGGGCAAGTCAACACCTCTTCCCATCCCGAGTATGCCCCTAATAACTTTATTGATGCGTCCACTGGTCCCTTAGGACAAGGCCTAGGAATGGCTGTCGGGATGGCCTTGACACAACGGGTACTAGCAGCTGAATTTAAAGCGTTAAGTCCCAAACTGTTTGACCACTTTACCTATGTGGTTGTGGGTGATGGTGATCTCCAGGAAGGGGTGAGTTATGAGGTAGCTCACTTAGCAGGGGTCTACCAGTTAAATAAGCTAATAGTGCTCCATGATTCCAACCGGGTGCAGATGGACAGTGTCGTGCGTGATGTGTCCTTGGAAAACCTGCAAACCCGGTTTACCAACATGGGGTGAAATTACCTTGAAACTAGTGATGCAGTTGCAGACATTGATGCTGCCATTGAACAAGCGAAAAAGTCCGATAAGCCGACCTTCATTGAGGTGCACACGACAATTGCTAAAAACACCACCTTGGAAGACCAACCCGCTGGTCACTGGTTTATTCCTACCGACAAGGACTTTGCGCGCTTTAACAGCAACACCAAGACTAACTTTACCCCGTTTGAATATCCCCAAACGGTGTATGACTTCTTTCACAAGCAGGTCATAGCACGCCAAGCTAAACCAGTGCAAGCTTACAAGGAATTACTGGAAAAACTAAAGGACAAACCGCTGTATACTAAGTTTATTAATTGAACGGAAAACGATTACCAGGCGTTGTACCTCAACCAGTTAGATGAACGCAAGGTAGCACAAACCAATGCGGCAACCCGTAACTACCTCAAGGACTTTTTAGGCCAAATTAACAACAGTAATTCCAACTTGTACTGTCTCAATGCTGATGTAGCACGTTCGTGTAACATTAAGTTAGGTGATGATAATTTACATACCAATCCGCATTCGCGCAACATTCAGGTGGGTATTCGGGAATTTGGTATGTCCACCATTATGAACGGGATGGCACTCCATGGTGGGGTCAAAGTGATGGGTGGTACATTCCTAGCGTTTGCTGATTATTCCAAACCCGCAATTCGCTTAGGGGCGTTAATGAACCTCCCCACCTTTTATGTGTACACCCATGACTCTTACCAAGTCGGTGGTGATGGACCAACCCACCAACCGTACGATCAACTCCCGATGTTAAGGGCCATTGAAAACGTTCAGGTTTGACGGCCTTGTGATGAAAAGGAAACAGCGGCTGGGGTTAACTATGGTTTGTTAAGCCAAGACCAAACTAACGTATTAATTTTGACCCGTCAAGCTTTGCCTTCCCTAGAACAATCTGATAGTGTACAGACCTTGAAAGGGGGCTATATTATTAGTAATAGAAAACAACCCGATGTCATTGTGGCCGCTAGTGGCAGTGAAGTTCAGCTGGCATTGCAACTAGAACAAGCTCTAAACGAACAACAATTAAAGACGCGGGTGGTGTCAGTGCCCAACATTAACATGTTGTTAAGTCAACCCCAAAGCTACTTGCAACAACTGTTTGATCCTAACAGTGTTTTACTCACCTTAGAAGCGAGTGCTAGCATGGAGTGGTATGCACTAGCAAAATACGTCAAAAAGCACACCCACTTAGGTGCTTTTAGCTTTGGTGAGTCCAATGACGGGCAAGTCGTGTATGAACACAAGGGCTTTAATGTGACTAACTTACTCAAGTTGATTAAAACTCTAAAGAGTTAAAATAGAGTAATGGTTAAGGTCTGAAAAATTGGGTTTGGGGTCTTTCTACCAACAGCGCTTTTATTCTCGGCCTGTTCGTTTAAGGACTATATTCCTACACCAAGCTTTAGGAAGGATTTCAGTACGGAGAACAATTTTGTTAAAAATAAGGTTCCCGGGAAGGATGACATTTACAGTAAATTTTACGATCTAACCTTTTCCTTAAACTTTGTTAACAACCAAGCCCAAGATTTTGGTACCGGTTGGTTAATTGACTGGAAGGGTGATGAGAATAAGGATCTAAGCAAAAACAAAGAAGGACAAACGGCATCTCAAACCAGAAGTTCCTCTGAACAAACTACCGATCAAGACGCTAACCTCTTTACTGCCTATATCGCCACTAACCTCCATGTCGCGGATGGTTTAAAGAATGATCAGGACTACGCTCCTTACAACAAGGATGGTTGGGGTCAACCATATCCTTACCAGCAAAAAACCCAATCCTTCTTATTAGGTAAGTACACCAAACCTAATGTCCAGTTAGTCAAGACTAACTATGAAAAGCCAGAGGATGCCGTGATTGAGCAGAAGCTCAAGGAAGATTCGCTGTTGTTTATCCAAACGAGTACGTTACCTAAAACAGCATACGCAGCAATTGATCCGGTGAACTTTAGCTATAACCCAACCCGTACCAACGGTTTTTGAACAGCCGGTAAATACAATGTTTACAATGGTGGTAACAGCATTGGTAACTATGCTGATTTTGCTGTGATTGAAGTACCGTTAGTACTATCCAACCCCAATGATGCCAAGATCTACCAAGAGTGGATTAGACCAGCTACCCAAGCCTACAAATATTTAGGTGATGTAGAAGGTTTGTTTGCTAAGAAGGGCTATCGCAGTTACATCCAAGATTTCTACCACTTATTGGGTTATCCGGTAACTAAGAACACTAAATCAGAGTTTATTTTGGGACAAAGTCAAGGTACTGTGAACCACATGAGTTTTAGTAATGATGAGTCCAATACTACTAATACTATTACTAAAGCTAGCTTAACTCAGCAACCCCACAAGGAACAATCAGCTTACGTGGTACGTGAAAGTGGACTGCCAACCCTAACAATGAATGTGGACAAGTACACTGGTGCAAAGGGAACTCACCTAGTTAACGTTGACCAAATTACTGACCTTAGTTTGGGTGATGGTTTAATTGACTTTGGTGGATTATCACGCTTTATCTTGCAGTACCACAATGTGAACTATAAGCAATTTGGTTATGGCACGATCTTGTGAGATACTAACTTTGCCGGTGGTTCTTCTGGTAGTGCCATCTTTAACCAAAACAAGCAGATTAACAGTATTTACTTTGGAGCATTAGTTAATGTAACTACCGACAGAAACGAAAATGTGGGTTTAGGCTTAGGGCAAATTCTCCGTGCTCCCAATACCTTCAACTCTTCCCACGAAGTGCCCTATTCTTATGACCTCATCTTTGGTGATGTTAACACCACCAATTTTTACGCCCAGTTTGCGAAAAAAGCACAACACCCACATGTGGAGCAAAATTCAAAGTACGCAGAATGGAGAAATTGGTTTCCACAAAAATTCAAAAACCGGTCAACAGCGGCATTAATCGGTTTTTGAGTAAATTTATAAAGTACTATTTTCTTTAACTAGTTGCCATGTTACTCCGCTCTGTTTGGTATAAGCTTGGTAGTCTGTTAATCATCTTACCGCTAACGGGTTGTGGTTATGTCAGACTAAAGCGTGCTAATTTCCAAACAGACTTTACCATTAACCGCATTCCCACCCAAGGGGATGTGTACCATGATAACTATGACCTGACTTTTTCACTCAACTTTGCTACTAGTTCCAAAGATTCTTATGGTACGGGTTGGTTAATTGACTGGAAGGGTGATGAGAATAAGCCTAGCAATACCGATCCCTTCCTGATTTACTTAGCGACTAACCTACACGTAGTCGATGCCTTGCGCAATCCCCAAGACTATGAACCTTACAATAAGGATAGTAATGGTCAGGACTATGGTAATCGTGATATAACCCATTTCTTTGCATTGGGTAAATACACTGATCCAGGTTTATTGGGTGTTGAAACAAAAGAGCAATCAGCCTTTATTTCGATTCAAACATCAGCAATCCCCAAAACCGCTTATACGGCTAATGATTTTGTTGATTATCAGTATGATAGTCTTACAAAACAGTGAAATAAAAAGAGTGATCAAAAAGATCAAACTCAAGAACAAACGGTAACAGGGCAAAGCTGATCTTACAAACCAGCTTATGCTGACTTTGCGGTAATTGAAGTGCCATTGTTCTTAGACAACGTCCGTGACAAACAGGTCTTTGATTATTTTGTCAAACCAGCCATTGAGACCTATAAAAAGCTTGGTGATACGGCTCAATTATTTACTGAGCAAAACCTAAAGGAATTGGAAAAGGAAACCTATATTATGCTGGGCTATCCGGTAGTGGAGAGCAATATTTATGCCCACATTTTAGGTCAAGGAAAAGAGTTGCGTGTGACTCAACAAGTCAACAACCAACCAGTTAAAAAGAACTATGTTTTGCAAACCATTACTAAAGAGCAACACACTTTGGATATTACGCGTGAAATACCAACCTTAATCCAAAACAAGAGTTTATCGGGTGATTTTGTTGGTAGTAAGCTCTTATCTCAAGAAGAACAACAGCAAGAACATGCTTTTTTGGGTAACTTAAACCAAGGGATAATTGACTTTGCTAGATTGTCAAACTTTAACTTGCAGTACCACAACCGCGAATACCAACAATACGGTAAGGGTTTAGCATTAGCTAACACTAACTTTAGTGGTGGTTCATCCGGTACCTTAGTTTTAAATCAACAAAAACAAATTTCCGGGGTTTACTTTGGCGTGTTAGAGTTTGGTGGCACCAACGGCACTAACCGTGAAAGTAGTATTGGTGTTGGTCAAATTTTGCGAGTAAAAGATGACGCTCAACTCCAACAACACAGTCACAACAACTTACTTTCACAATTAGGTTCCAGTCATAACTCGATTACTTACGACATTATTTTTGGTAACAAAGACACTAAAAACTACTACGCCCAGTTTGCCAAAAAACATCAAACACATTTGTACAGCCAAATAAGTTCAAGTAATCAGCAAGAACTCAAATACGTCGATAACGATCCAAATCTCAAGATTAAAGAAGAGGCTGCCAAAAGCACTGTTCAGAATTTAACTGTCTACAGTTAATTACTTTTCGCATTTCAATAATTGAAGAGTTGGTTAGCACAAATTTAAATTTAAGGCTAGTTTAATGACTATTTATTTTTAATTAGTCAACAGTGATTTAAATTTTATGCAATTAACTACAAGTCAACAAATGCAAACTAACTTTCTTACTACAAATAAACATCACTTATTGAAATACACCAACGGTTTGATTTGGTGTTGGTGATTATTTGTTATTTCTTTAGTTTTAGCTTCATCGACATTTAGAGGTTTCTTTCTCGGCACTATCAATATTGTGAATTTTGTGTTTTGGATATTAGCACTGATTTTTGGTGTAGCTGTCGCTTTCATTAATGGTGTTTTGAGTAGTGAATTAAAAGAAAATTCAGTTTTTCAAGAAGAACAAAAACGATTCTTCTTGGGCTTTTTCTTTCCACAAATGGCTTTCTGTAATGCGCTTTGACTGAAACTTAAATTAAGCTATCTAAATTCTGAAAGAGAAAACTTATTAGAAAAAATAAAGCAAAAACTAAAGAAGCTTACCTTGTCTGTTTTTGTTGTTTGAGGAATTTATTGTGTTTTAGCAACCTCAATTTATTTACCAAATGCATTAAGAATTTTAAACATTTATCAAATACCTAACTTAATCGCTTTAATAAATAATAGATTGAGTGAAATTTTGCCAGATGGTAACCGTTATTTTTTAGGTCATTCTTCATTCGCTTTTCATTACTATGAAATAGTTTCAAGGATTCCTTTTTTAGTTTTCTTTATTATTCCAACAATAACCTTAATTACTTTAGGTTGCTATTTATTCGCTTACTTAAGGTTTATAAACTCAAATAAGTTAAGAAAACCACTTTCTACTTTATCAATAGTTATCATGCTGACTGATGTTGTGGGAATAATTCAATGAATAATTATTGATATCTTATTAATTTGGCTTAATGTACCCTTTGTTATATTTGTAATTTTTTGAGTAATAAAACTTGTTTTGCCTTTAGCAATGATAGGTACGTTTGTCAGCTCCTTAACTATTTACAAAAAAGTAACAAGCAAAGAATGATTAGCAATAAAAGAAGAACAAATTAATTTAACGACAATGAATATTAATATGGGAGAGCAATCATCCAAAAACATGAATTCTTTTGAAAATCATGAATCAAATGAACGAAATAGTCTTCAAATTTATCAACAACATTCATCTATGATGTCAGAAACAAAGAGAAAACAAAGTAGCTTAAGTTATGATGCCAGAATTCTTTTACCTAAAAGTCCCTATAACACAAAGAAAACTTTATTTTTAATTATTTTCTTTTCCATTATCTCCTTGATATTGGCAACTATAGGTAGTGTGTTCATTTCTTTTGCTATAGTTCAAATTTCTATACCTTTTTACGTTATAGGTGGAGTTATTTGATTTTTTACTTTTATTTTCTTTATAGTAACTTTTGTAGCAATTGTAAGGTTCATAGCAGAGTACTTCGCTAATAAGTTAAATGCACTTACATGAAAAGTGGATCTTTCTGGACTAGAGATTAAACCTAAGGAAGAAGAATAGTTATTGATTAAATTTGTTATTAAAAACCAAAAATTTAGTTTTTAAATAACTTTCTGTCTCTAAAAAAACGTTATCTTGTAAACTCAAAGATAGTTTCAGATTAGCTTTGCTTGCAAAAGTTTAATCTGTAAAAAGTTTTAATTCTATGGAGTCTGCACTAAACCAAGAGTTTCAAATTGATTTTTGTGTAAAAAACAAAAAACTGTTAAAAATTTTAGCTAACGTTCTTATTGCTTCTTGGTTATCGTTTGTTGTTTTTTTAATCCTAGGTTGTATAGCTATTGATCTTTTTCGATTTGATCTTTATTCCCAATTTTTCTTTAATCACTTAAGTACTTTATCTGCTTTAGCATGAACGTTTTTTGTATTAGCAATACTTTTTGGAGCAGCTACATTAGCCATTAATGGGTTTTTCTATAAAGAAATAAGAAAAAAATCAGCTTTTAAAGAAGATTCTAAATAGTTTCTTTTAGGCATTTTCTTTCCCCAACTTGCTTTTTCCAATGCGCTTTTCCTTAAATTTAAGTTAGCCCATTTGGATTTAAAAAAAGAACTTTTATTTAAAAAAGTAAAGCATAAAATAAAACTAATTAGTTTATTTATTTTTGGATTTTGATCAATTTATGTTGTTTGCATGACTTTAATTTATGTGCCAACTATACTTAATTTAATTGATAGTTTCAATTATTCCGAATCCATCTATAAATGAGTTGACTATTTCTTTCGCCATTTAGAAAGTAGAGATTTTTATTTTAGTAACTTTGGTTTTATATCACTCTTTTTGTTGCTTTTTGTTATTCCAACAATCACTCTCACAACATTAGGTTGCTTTCTATTTAGTTATTTACGGTTCACAGATATAAACAAAATAAAGATACAGATTTACAGCTTGTTAACTGTTTTCATTTTTATTGATGTTTTTGGTTTAGTGGTGTCTGTTCTGTTTGGTTATCTTTTACCTCTTGCTTTTGACTCACTGCCTTTTTCAGTGAATTTAACAAGAGAGGTGTTTTTGTCTTTAGCTATGATAGTCATATTTGCTAACAGTGTCATTTTTACTTTAAGGCAAAAAAGAAATATTGATTAATTAGCGAAAAAGAACGATTAAAGCTTCAAGCAATTGCTAATTTAAATATGGAAAAAGAAACAAAGAAAGATTTATTAAGTGAAGTGAAAAATAGCTTTCATTCTTCTAACAAAAATATTAAAAGCGAAATTATTTTGAAGAACACTTCAGAAAATAATATTTATTTAAGAAGCGATGCGAGAAGGCTTTTACCTAGAATCCCTTATAACACAAAGAGTGCATTATTATTCACCCTTTTATTTGCATTTCTATCTGGCCTTTCTTATGGTCTTGCATCTTTATGTATTAACTTCTCGTTTACTTCGCCACAAATTTTCATTGTCTTAATTTATTAGGGGTTCTTTTCTTTATTGTTGGTCTTGTTTTGGGAATTATTACCTTTATATGATTAATAAGATTCATAGCAGAATACTTTGCTAATCGTTTTTATGCACTTAAATGAAAAACCGATGTTCCTAGTACGTAAAATAAATCGAAGCAATAATTTAAAAATTGTTATTAAAACAGAATTTTAGTTTTTATCTAGATTTCCGTTTCCAAAAAAATGTTTCCTTGTAAATTTAAAGAAGTACTTTAAATAGAAAAAAGTGAAACAGAATTTAAGTAGTAGAGACTTTATTAAAAAGTCAGAAAAAACAGTATCAACTACTGTAAATATAAATACAGAAGCTAGTAGTTATATTGCCAAAAATCTAGAAAACCAAAAGGCAGTTCTTTCAGCAAAGGAATTAGAAAAACAAGAGCTTTTACTAGTGCGCTTAAATGCAGTAAAAAGATTTCCGTGCTATCCATACAATACAAAAAAGGCATTTAATATTTCTGTTTTATTTGTTCTATTAGCTGGTCTATTTTTTACTGCGTCTTATTTCTCTATTTTTTATTCGATTTTTACTAGGTTTAAAGCTTTACTTATTTTCTTAGGAGTAGTTTTTATTCTTATAACTTTCATTATGCTAATTCATTGCTATATATGGTCAGTAAGGTTCAAATCAGAGTACCTTGCTAATCGTATTTATGCACTGAATTTTCAAAAAGGAGCCTAATATAAGTGTTCAACAAAACCCAACAAAAGAAAACGTTAGTGATGTTAGCATGATAGAAATAAATAATTTGACCGCTTTAGAAAGGGATGAAAAAGTAGAAACGGAAGACATTAATACCAATGTAATAGCAAATACTTATACAGTGCAAGCCAATGTTCCAACTGCATTTCAAACCAGTGAAACAAATAGTAATACTGTTACTAACGTCCCAAAATCTCCAGAGTTAATAAGTATTCAAAGATTAGAAAGATTAAAAAATAGGTATACTTTCCTATAATTTTTTTCACTTTTAATTGGCTTCATTTTAGCCATATTACACATTGGAGGCTTTACTTTTGCAGGTGGTATTAATTTATTGGCCTTTTTAAGTTTTATAGATGCAGTTGCTTTTATTTGTTTTATTGTTTTCCTGGTTTTAGCAATTTCTACATCGCGAAGAATTAGAGACTTAGAAATGATGCAACAAATGAAAGAACTTGTTGATAAAAAGCAAGAGACACCAGAGCAAAATAAACAGTAAGTTATTTATTTTTAAGGGTTATTTATTTCTCTTTTTAGGAAATCCATTTGAAATATTTTGCGAATTTAGTTTATTCAATTCAAAAGACTTTTAAAAGAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATTAAAGATATTTGTGAAGCTTCACATGGAAGAGAAATAAATACTAAATATCTCCGTGAAAATCAAGGAATTTATCCTGTTTATTCATCAGCAACTTCTAATGAAGGTGAAATGGGCAGAATCAAAACTTACGATTTTGATGGTGAATATGTTACCTGAACTACACGTTGAAGTTATGCCGGTAGCATTTACTATCGAAATGGTAAATTTAGTGCTTCCTCAAATTGTGGAATTTTAAAAGTTTTAAATAAGGAAATAAATCCTAAATTTTTAGCTTATGCTTTAAAAAAAGAAGCAAAAAAATTTGTTAATACAACTTCTGCTATTCCTATCCTTAGAACGCAAAAAGTTGTCGAAATTCCCATTGATTTCCCACCCTTGCAAATTCAAGAAAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATTTACTTAACCCAAAAAATTGAAAAGAGGAAAATAAATATTACAAGCTGGGAGAAATAGCTCAAAAAGTTTTAGTAGGTGGTGAAAAGCCTGCTGATTTTAGTAAGGAAAAGAACGAAGTTTATAAATATCCAATTCTTTCTAACAACAGTAAAGCTGAGGAATTTTTGGTTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGCCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTACCTAAAGAAGAATTTGATATAAGGTTTTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCTCAATTTAAGGAGTATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCTGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTATTCCTGCTGAAATTGAACTGTGCAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTGCAAAACCAAAAAGCAGCATCAATCCTGTAAGCAAAGAACTTTTTCTCTATGTTCTTTTAGTGCATTTTCTAACTGAAAATATCCAAGTAATTAGTTGTATTTTTCAGAAAGCTTAACTAAAATTCCATTGATTCGGTAGTTTGTTTATTTAAGTATTTGGCCAACATGACCAAATAAATTTTTTATGCAAACTATCTTAACTGATCAAGCTAGCTTCAACATATTAACTAGCAAGAGAAATTTAATTAATAAATTAACTATTACAAACATAGTGCTTTGAGCACTGTTTGTCTTTTGTGCAATAGCCACAGCCTTTTTAGCTATCAGCCATTCTTTTCACAAGAATTTTTCTTTTTTTCTGGAGTCTGGAAATTCAAACAACGAAGGTGAAACAACAACGAAAGTTAATATTCACGTTCTTGTAGCTCAAATATTAACCCCCATTTTTACAGTTTTACTGATTCTTTGTTGAATGGGCAACATCGGGATTAACAGTTTATTGTTCGGTCAACTAAGTATTTGCCAACAATTTCAAAATGAGAAAAAATGATTATTGTGAGCCATCTTTATCCCACAACTCACACTAACTAACTCACTGTTAGTAAGAGAAAAACTGAAGAACATTTTGGATGCAAATAGCAATAGTTCTACTCAAAAGAACAGACTTACTTGATGGATGATTGGCTTTTTTGTTGTTTGATTTATCCAACTTTTTCTAGGTTTCTTAATTTATTTACCAGCTTACAGTGCTGAAGCTCGCTTAAATTTAAATTTATCTTTTTTATCCTTTGCCTTAAATTCACCTTTACCAGTAGGGCACGCAGCCAATGGCTATTTTTTCTTAGGTTTGATTTTTGCATTTATGGATTTAACCATAATAGTACTCCTAGTTATTAGTGGCTTCATCTTGAAGCATTTAATTCAAACTAATAAAACTGAGCACAAAAAATACCAACTAATGTTTTTCTGAACTGCTTTAGCAATTGATGTAATTGGGCTAATTATGACCATTTCATTTATAGTTGTTAGCTTTTCCTTAGAAAAAGGAGATGACTACTTATTAAAAACACCTTTCTTTGGTAGTTCATTGATGGCATTAATTACTATTGCAACGCTTGTAACTACAATCCTTTTATTTATTAGATTTAATAAAAAAAGATTACTTAAAAATTAATAAATAGTTTCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTTGTGCGCGCCGCCCATTTACACACCTCATACGAACTCGTGGACTGAAAGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGAGACAACCAACGTTCCCAAAAAAACCTCACCAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGACCGGTGTCGAACCCCTCGATTCGACCAAGGCTGGCAAGGGGAAAAGTTCCACAGAATGAAAAACGGAGGAGACAAATGCTAAGAGTGATGCTCCCCTAGCTAATGGTCAGCAAGTGGGGGCTGGAAGTGCTTTTGGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAGACCGCTCTTGAAGCACACCGCCCAAATTAATTTAAGGCAAACCCAAGACAACGCGCAAACTGGTAAATTCTCCAAATACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACTCGGCTCGACTTCTACAAATGAAGATCTGCCGAATGTAATAACAAAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTTGTATTGGGTTGTCGGGGAGGACCAGGAATCGGGTCGCGCAACCTGATGAGCCCACACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGTTTTAAAGATGACTCCAATTCGGATTCCAAGAATTCGAATTTGAAGGCTCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGAGCACGGAAAACAGTGCTGCCACCAAAGACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGCTCAACGGTCCGATTAATGGCTTGTTTACCCTGCTCGACAGCTTTGCCTATGTGACCCCGGTGAGTGGCATGAAAGGGGGTAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGCGCGCTTGCCGTCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAATCAACTTCCGACGCTAATTCGAACACCAAAGTCAAGTGAACCAACACCGCCTTGCACTACCTCCCCGTGCCGTATTACTATAGTGCCAATTTCCCCGAAGTCGGTAACAGAAGAAGAGCGGAGCAGCGGAATGGGGTGATAACAATAAAAAGGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTTAACACAAGTCACTCTATCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAATAGCCAAAAAGTTTGAATATTTTGCAATTTGGGTTTACAACTCCATCTAACTATTAGTAGGAACTTTTACTTTCAAAAAAACTTTTTGGTTTATTTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTTAGTGTAAGCACAAAAACCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCACTACTGCAAGTTGTAGTTTTCTCAACCTCTTTCAGAAAGGAGTTATTGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGATGAAAAAGACCAAAAAGGGCACTTTTTCGGGATGATACATCATTGACAAAGATAACAAAAGTGTGGAATTCTCTGGCAATTTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACAGAATACGTAACGCGTAAAGAGTTTAATGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGAAAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGTTTGAACAAGATGGATGTTCGTCTAGATAAACTAGAGTCTAAGTAGATATTATTCAAAATAAAGAATACCAGCAATTAGTCTTTAAAAGATAGTTGTTGGTATTTTTATTCATTTAATACTTTCTAAAAACTTTTTGCAATTTTGTTGGCACTTCTCAATAAGCCGGTCAATTTATTTAGTGGAAGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACTTAGTTGAAAGTTTTATTTACTTTCCAAACCTTACAAAGAAAGGAGGATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCGAAGAAAAGCTTAAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTACTGATAACGAAGTAAAAAGCGAGGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTGATTTTTTGGTCTAAAAAGACATAAATTTACCAACAGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGACTGTTTTGGACCAGGTGTTGGATTATGTTCCCTGGATTGGGAATGGGTACAGGTATGGGAATGACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAACGTCCAAGCGACGCTCGGGGGCAGTCAGACGACGACTACCAAGGGTTCGCCTTGAAGAACCCTCGACCCAGCCAACCTCCAGCTCTGAACCGGCGCGGGGTGAAGGAATGATAAGGCTTCAAACAAACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGGATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCCGATGCGTTAAAGCAGGATAAAATCACCAAGGAAAACTCGGGCAATCTCAAAACGGAGGATGGTACTGCTACTAATTCGACGACGGCATTCGCGTCGAATTACACCAACCTCCCCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAATAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTCAATAAGAGTGGTGAAAATAACTAAAAATTCCAAGCCACCGACCAAAAATGGTCGTATGCCGACTTAAAAACAGACCAAACCAAACTCAACCTCCCCGCTTACGGCGAGGTGAATGGGCTAATATTTCTAAAAATTCAGTTTATAAGCAAAACTTATTAGTTAATCAACAGTTTGAAAAAGTTTTATTCTGAACCTATTAAATGTTAATAAAAGTTAATAGAAATAATAAAAAAACTTATGTAAGTATCACGAACACTCTTTTTAAATTCTTAGAAAAATGCACTTTTTTCCACTTTTTATAGCGATTTCTTTTGCATTCTTTAAACATAAATAAAAAGTTTTTCTGTATAATCTTCAGGCTGTTGACAACTCTGTCGACGCCGAAAATGTTCTTTCAAAACTGGATGCAATCTGTCAATTTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGAAAGTAGTAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAGACTAGCTAATACCGCATAAGAACTTTGGTTCGCATGAATCAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTGGGGTAACGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTAAGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTTTAGCAGGTAATGGCTAGAGTTTGACTGTACCATTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATTAATCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGGGCGATCCCCTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTCTAGCGAGACTGCTAATGCAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTCGCCAGCTTGTAAAAGTGAGCAAATCTGTAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGTCGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCATTAGGAAGCGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTTCTAATGGAGTTTTTTACTTTTTCTTTTCATCTTTAATAAAGATAAATACTAAACAAAACATCAAAATCCATTTATTTATCGGTGGTAAATTAAACCCAAATCCCTGTTTGGTCTCACAACTAACATATTTGGTCAGATTGTATCCAGTTCTGAAAGAACATTTCCGCTTCTTTCAAAACTGAAAACGACAATCTTTCTAGTTCCAAATAAATACCAAAGGATCAATACAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAATAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGTAGGTGGTAAGAACCTCAGATCCGGAGATTTCCGAATGGAGCAATCCGGTAGTTGGAAACAGCTATCATTAATTGATGAATAAATAGTCATATTAAAGCAATACGTGGTGAAGTGAAACATCTCAGTAGCCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACTTATCATTAGATAGGGGTTGTAGGGCTTGCAATGTGGACTTGAAAACGATAGAAGAAGCTGTTGGAAAGCAGCGCGCAAAAGGGTGATAGCCCCGTATTTGAAATTGTTTTCATACCTAGCGAGATCCCTGAGTAGCTCGGAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAAAGAGCGTTTGTTTGTTATTATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATCAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTGAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTCGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATTTATATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAAAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTTTCCACCCCCATAATTGGATTTGGGGATAAATCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACATTGAGTGATGATGTCGAGTGAATGAGTGATCAAGTAGCGAAGGTGGTATTAATCATGCTTTCAAGAAAAGCTTCTAGGGTTAATCTAGCTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATGTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAGGACATTATCATGTAGAGAATAGGTAGGAGCAATCGATGCAAGTTCGCTAGGACTTGTTGATGCGAAAGGTGGAATACTACCCTTGGTTGTGTGCTGTTCTAATTGGTAACTGTTATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAATGGAGGCGTACAAAGGTACCTTCAGTACGGTTGGAAATCGTATGTAGAGTGTAATGGTGTAAGGGTGCTTGACTGTGAGACATACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTCCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCGTAGGAAGATTGAAGAGTGTTGCTTCTAGTACGAGAGGACCGAAGCGAGGACACCTCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCGCAAGAAAGTAAGAGCCGTCAAAGACGATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGAAGCCTTTGGTAGGAAATACGCAGGATTGTCGTTTTTGTTTGGTGCCCATGTCGCTGTGGAAACACCTGGTTCCATTTCGAACCCAGCAGTTAAGCACAGTGGAGCCGAATGTAGCTGTTTCAGTGAGAATAGGAAAGCACCAAGCGTCAAGAAGATAAAAGAGACTTAGGCCTATGCCTAGGTCTTTTTTATTGTTTTGGTAAGCCAAAATAGTCCATTTTATAGCTGTTTTTACTTTGCGAAAAAAACAGAAAACTCCAAGCAATCAATTAAAGCTTTGAGATAATTAAAGGCTTTGGTACTAGTCAAAACCAAAGCCGATTTTTTCATGAACCAACAATTAAATACCACCAGGAAAAGTACAGCTGCTAGGGGCAGAATGGGACTTGTTGGTGGTATTTTACTCGTCATTGGTACCTGCATAGGGGCAGGTATTTTCTTTAAGAGTGAACGGGTGTTGCAAAACATGGGCGGAAACACCACTTTAGCCTTACTGGTGTGGTTAATGGCAGGGATTACCGTGATCTTAATGGGGTTGGCGCTGGTTGAAATTACTGCTAAAGCAGCCTTTGATGACTTGGCATTATTGTCATGAACACAAAAGTTTACCAATAACACCTTTTACAAGGCCTGTAAGCGCTTTTTAATCTGGATTTACTTGCCCACTACCTTCTTCTTTATGCCGTTGTATTTGGTGCAATCACTCCAAGATGGTTTGCGTGGTTTTGGGGTCGCCAACCACTTCAATACACCACATGATTGGGCCATTTGAATGGTCATAGTGCTGTTGATTAACCTGTGATTTTTCTTTACGAGTGGTCTTAGTGTTAAGTGAACCAGTGTGCAGAATGTCGTGCTATTATTGCTCAAAGTAATTCCCCTAATAGCAGTGGTTATTTTAGCGCTGTGATTGGGTGCAAGTGCTGAACAAATGGAACGCCAACCAGTGGTTCCAGTCAAAGATTTTACTGCCATTTTACCCTTCTTTGGTTGGTTTAGTGCTATGGGCGCGATTTTCTTTGCTTTTGATGGTTTTTACGTGTCAGCAGCAGCGAAAACGCAGTTGAAAAAACCAAAAAAATTACCGCAAGTAATGTTGTTGGGCTTAGGGATTGTGGTACTGATTTACAGCTTAATTGCACTAAGTGTGAGTTTAACCACACCTAACGGTGCCTTTTCGGGACTTGGTGATTGGTTAAAGCACAAAAAGCTTGGTTGATTCTTTGGCGTGCTCAATCTCTTAATAGCGCTTGGAGTAGCTGGGATTATTAACGGTTTTGTAATGTGAACGGGAAAGTTAACCCAAAGCTTAATTAAATCAGGAGAGTTGTGGGTACCTGATAAGTGTAAGCTCTGTTTAAATAAGCCCAAGCCAGTAGTGGGTTTAATCCATGCAGGGATTTTGATGGTGTTAACTACCGTAGCACTTTCTAGTTTAGGTGGTTTGTTGTACTTACCTAAAGTTAATGCTTCCTATGACGGCAAGGGCTTTAAATCAATGGGGTGCTTACTGGAGTTTGCTGACTTAATTGCCACTTGGACTAGTGTCGGGATCTTTTGGTTCTTGGGCTTAGTTTTACTGGGTGGTTTATTGCAAATCAAGAAACCGAAGCGGTGGTATTTTAGAACAACGGGTTGGCTAGCTGTAGTAGTGATTGGTCTCACTACTTTAGTGGTCATGGTGCAACCGTTTGTAGATTTAGGAATAGCAGTGTTTAACCGTAGTTATGAACGGATTGTGGCTAACACTATTTTGATTGCTATTCTTGTGATAATTGTTTTAGTAATGTTCTTTCCAACCGAACCAATTAAGCTAAGACTTTGACGCAAACGAATCCAAGCCATGGAAGCGTGCGGTGAGGATTGTGACGCTTGCGTGGAGTATTAACTAAAACTATAAAAAATAAAACTGGATAACCAAGCATGTAATATGAACAAGTTAAAGCGGCATAGTTAATTTGCGCTTAATTTCTTTTGGGGAGTTACACCGGGTAGAGGGCGAGTTTTGCTGGTATGCTTAGCGCGTTTTTGTAAGATTTGGCTTTCTTTCTAGTGTTGCTGAAATGCAATTTAAGTATGGTGCGCTAATTTTTAGCGGCTTTTTAGGACTTTCAATAGTTCTAGCTAGTTGTGGGGCGAGGGGTAAATTTGATCAAGTTGATGATGGTAAGATTAAGCTAGCTTCATCTTTAACTTCTAGAAGTGCGTCAGCGGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGTGTAAATGATTACCCCATTGAGATTACCCAGATTGCTGGTGGTTATGATGGCGGGCGAACTGATCTCCAAACCCGGGTTAGTGTAAAGGATAAAACCAGCTTTTACAACATGATTCTAAACTACCCTGATTTAGTGTCGGTGTTAGCCCGTAACGGGATGGAATTACCGTTTGATGGAGTCAATGTTGACAAGTTATCTCCCAACTTCTTAAAGTTCAATGAACGCATTAGTGGCGTAGCGAAAAAAGCTAACTATGCCATTCCCATTTCGATGTCAACCGATATCTTAATCCTGAATGCACCGGTGTTGCACTATATTTTGAACAGTGTTAAGAAGAATGATGGCAACACTAAAGTTCAAGTTAAAGCTCAGTCAAAAGATTCACAAACAAAAGTTAAAGGTACTATGGAAATTGGCACTGATGAAAGTACTAAAAACTTATGAAGTGATATTCAAAAGAAAGCTGGCGAAAATGGCAAAGCTACTACTGAAGGCACGAAGAAGGCAGCAGCAAAGTCTACTCATTTAACTCTGTTAACTAAGTCGGAACAAAGTACTCAAGGTAATAATGGAGCATCTGAAAGTGATAAAAAAATTGAAGAAACTTGGGGTACTTACAGTGAAGTTGATGGTGGTCTAAAGAATTACACCTTTAAAGCCGATGTGTTTGATACTTGACATGGGTTGATCGATTTTTCAACGCGCGTAGCAAAATCATTTAAAAACAAAGTTAGTGATATTTCCACTAAAAAGGGCACTGACATTCAGGGCGTTTTAGGTCTAGACAGTACACCAAATGCTTTGTTTACCTCAGTTTTTGCTGCAGGTGATAGTAACTTTGATAACTTCTTTTATAAGGTTAAAGATGGTCGCGCTGACTTTAGTAACTTTAACGAAAACGGTACCTCTTACAAAAATCTGGAAAAGGTTTTTAATGACTATAAAAAACTCACAGACAGCAACGGTTTGTTTGTCAATAAAGGGGGTTCTTACACCTCTAACTTCCAGAAGTTTCACCAGTTAGCCTACAGCATTTCCTCCTCTTCTGGTTATGCTTATGCGTTTGCTGGTGAAAATTCAAAACGTTTAAAGTTCAACGACGATACTTTCATTGAGTACCCTAGTTTTACCCAGGAAATTCATGCGCCTGGTCAAAGTAGTCAAAAAGAAGGTGGACAGCAACAAAGCAACAGTAAAGATAACGGCAATCTTTTAGGCACTTTTACTATTGAAGCAGCGAAAAGTAAAACAAAAACAGAAGTTAAAAAAACTGAAGACACACAAAATCAAGGCAAGAAGGCTGAAGGCACACCTAACCAAGGTAAAAAAGCCGAAGGGACCGAAAACCAAGGTAAGACTATTTTCCTTTATAAAACATCAATTCCTAATGATAAGCAAGATGGTGTTGACGCTGTATTAATTAAAGACAAGTAGTTAATTCAAAAACTAGAGCAAGCTAAAAATAATAACGGAGCCGAGAAAAAGGATAAGTCTGAAACTAAAACCAATGAAAACAAAGGCACAGTCATCGGTTACACCACTACGAGTTTCCTGAATGCTGACAAAAAGCATGTCTTTGAAATTAGCAAGCTTAATGATGAAAAATACGACCGCAAGATTATCGTTGGTGCTACTGAGGAACAATTAGATCAATCAAGTACGTTACAAAGTGCGGAAGCAATTATCCTGCCAGCACCTGGTAAATACAGTGCCGAAAACCAGCATAAGGTAATGATGACTCAAGGTCCTAATCTAATTGGTATCCATACTAATACCAAGGAAAATGAAGAAACGCAAAAGTTTGTTAACTGGTTCTTAAATACCAGTCTAACTTGAGATAACAACGAAAGCAAAACACCAGCGCAGTACTTTACCGAGTCTGCTTCTTACATCCTGCCTTTAAAAGAAACTTTTACCGGCTCTAACAACAAAGGTCAAAGCGGCAAGAATGACGGTAAGAATTCCAATAACACCTTTAAAGCCAAAGCACTAGAGCTCTTTAAGGAACAGAGCGAAAACAAAATAGTGGGTTACAGTGATCCAAGCGATTTCAGGGGTGGTAAATTCCGCGAATCAATTGGTTCTGCCTTTAACGCCACGGTCAATTCACACGTTGACTTTAATACTTTCGTTGCTAACTTCCGTGCTAACTTAGGTTCTGGTTACGATAAATAGTTAATCCCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTCGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGCACAGGTATGGGAATGATCACCGGGGTGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTTTCGAATGTCGGCGTCGGTCTCAAAGCGAATGTCCAAGCCACCCTCGGGGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGACCCACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTACTGGGAATAACACCTAAGGCAACGAAAACCACACCAAGTTCGCGAGTGCTACGGGCACGGGCCAGCAGCAGGGTGGTTCTTCAGGTACAACTAATTCCGCGGGGAATCCCGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGACCGGTCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCTCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCTTTGGTAGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGCACCACGGTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGGCTCCAGTTAAGTGGCCTGGATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGTTGGGCCGCGTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTGCCACCACCGCAACAGGGGCTACCTTACCGGAACACCCGAATGCTTTGGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCTCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTCGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTGTTTGGAGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGGCAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACCATTAATAGGAATTTTTCTTATTAATAGGAACTTTTCTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAACGAGGCTTCACCGCTTTTTAAAACAAGTTCAATTGCAACTTGTTCCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTATTGGCGCTTCTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCAGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGGTAAAAAAGACCAAAAAGGGCACTTTTTCGGGATGATACATCATTGACAAAGATAACAAAAGTGTGGAATTCTCTGGCAGTTTTAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAATTTAACGAATATAAAGTTTCAAACGACCAACGGCTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAGGTTGAGCAAATTAAAGCTCAAGGAAAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCACTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAGTTTTTAGGCTAATAACAAAAGTGCCAGCAATTAATTTTATAAAGCTTATTTGCTGGTATTTTTATTCACAACCAATAAACAAATAAAAAGCCATCCACCGCGAGGTGAACGGTAGACGGCTAAAAATTTTTAATGATTTTGAAAACTAACGAAAAGTGTTAGTTTGAGTTGTAAAGCAGTTATGAATGTTTGTTCCTTTAAAAATTTGTTTGTTGTTTAGCTAAATATGTGCTGAAAGCCATTGCGTTAACATAAATAAATTAAGACATAAAAAATAAACACATTAATCAAATAAGTGTGGGAACAGAAAATCAGGCTTTCAACACAAAATTTTCGTTTTTCTGCTCCCACTAAATAAATTGAGTTGGTTTTTAAAAACCGCCAAAAAATTTTTAATTTAGTTTTTGCTACTTTCCCCTCCCCCTCACGGGTGAAAACCCCGGCGCTTGGGCCTTAGTGCGCGACAACAGCGCCAAGGGCATCACTGCCGGTAGTGAAAGTCAACAAACCACGTATGATCCCACTCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTAGTAGGTTAAACCCACAGACACCAACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGAACAAGGCACCGAACAGGTGGAACTTGAATCTAATGATCTCGCCAACGTTCCCATTAAGCGGGGCGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGAAGGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAATCGTACCGCCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGGTTGTGGGACTGAAAGGCCAGAGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGGCGCCACCCCGAGTGGTTTGACCAGGGCCAAGCGGTCGCGGATAATACCCAAACCGGGTTTGATACAGATGACACCGATAATAAAAAACAAGGCTTTCAAAAGGAAGCTGACTCCGACAAGCCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAATTAATAAATAGGTCTATGGATTAATTACCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGGGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAGGCTTAATGATGGGACGAGTTCGGATTCCAATACCACCACTGATGGGGCGCTTACGTTTGACACGAATGAAAGTCAAAAAGCTTTAGGCTCCACGGACACGCAGGGTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGAGACAACCAACGTTCCCAAAAAAACCTCACCAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTTCTAGTACCAGTTTGGGCAATGTTGGGGACACCACTGCCCTAACTCCACTTTTAAGAGGTGCTACTACTACTACTACCGTCCAGCTAAAACAAACAGATTCTAACAGTCAAGATCAACAAAAATTCCAAAAATATTTAAACACCGCCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCAGAACTTAGCCAAGGCGGTTGGCAAAGCCAAACAGCGCGGCACAGTTCCACCCGGGGACTACAGTCAGCCGGGGCCAATCGTGGCGCTAGTTTAGCGGCACGCACCACCACAACTGTGGGGGTGGGTAGAAGAGATTCCACTTCCACCACCTTGGAACTACCAAACGTCATCACCCAGCTTTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCGAAGACACATTTGAACTTCGGCACTGAAGTTCAAAAAAACTTCGTCGAAAACCAGTTGGGGTTTAAATCCGAAGACAACTCCAACACCTCCTTGACGAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACTTAATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAACAACCTGGTGACGAATACTGCGGGGTTAAACGGTCCGATTAACGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATAACGAAGAAGTGCAAACGACCTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCGTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATAATGGCGATAAAAAAGCCATCCACCTAAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACTATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTACCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAGTAGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTACAACTCCATCTAACTATTAGTAGGGTTTTCACTTTTGAAAATTTTTTTGGATTAATTTTGGCACCCCGCCTTTTTTAAAGGCATTTAAGAGATATAAAAGAACAACTCATGTTTCTAGATACTTTCAAAAGATTTTTCGGGTAACTTAGATCACCTTTAATGTTGTTGTGGTTGGAGTTGATTTGGTTTAAATGGCAAAGGTTATGGTAAAGGTTTTTGGCACCTTTCTTTTACTTGTGCAATTCATTTATTAGAAGGGCAACAAATGGTCTTTTTGAACTAATCAGTTGTTGCCTAGCTTTATTTATTTAAAAAATGATGAGTAAAAATAAATTCTTTACCATTACTAAAGAACAGTACAGGAAGTTTACACGCAATAAAATTGAACTTCTTTTTAAGGTCCAATGTACCGAAAAGAAAAAGGGTAAACGTAGGTTCAAAATATTTCAGCAAGTCGAGGATAAACCAAAACCACCAGTTAAAATAATTGAAAAGGAATCTAAGGGACCGGAAGAACCAAAGGATCCTGGTATTAAAAATGGACCTAAGGGTCCCGGTGGACCAAAAGGACCTAAAAAGCCACCTGTTCCCGATGATCCTGATACACCAGATGTTCCTAAAGGCCCCAAAGAACCAGGCGGTTCAAAAGGTCCAGGTGGTCCCGATGATCCTAATGGAGGAAAGAAGAAAGTGCCTAAACCAGATAAGTATGTAACCCATCGCGAGCTTGATGAGAAACTTACTGAGTTTCATGACAAGCTTATGGTAGAACTTGATGGTAAGTTTGCTACTAAAGCTGATTTAAAACGGGTTGAAGATAAAGTTGATGTTTTGTTTGAATTGCAAAAAACGCAAGATGAACAAATCAAGGTTCAAGGAAAACAAATCTAACAGTTAACTGAAACTGTTCAAAAACAGGGCGAACAAATAAAGGAACTTCAAGTTCAGGTAAAAGCCCAAGGTGAAGAAATAAAAGAAATTAAAGTTGAACAAAAAGCTCAAGGCCAAACCTTGCAGTTAATTCTGAAAGCACTTGAAGGAATAAACAAGCGACTAGATAATTTGGAATCGAAATAATCCCATTCCAAAATAAAAATACCAACAGTCAATCCTTAAAAAGTTCACTGTTGGTATTTTTATTCACTCAAAAAATTCTTAAAATTTTTTTGTAATTTGTCGTCACTTCGAAATAACTGAATCAATTTATTTAGTGGAGGAGTAATTATGAGTAACTTTGAATTTTGATTAATCCTTCAGAAAGGAGGATTTGATTATGAAAGATGAAACAAATATTTGTTCTCAATCTTTAAAAAGACCTTTAAGCGAAGACCAATTGATAGAGTATTTGAGTACTTATGAAATAACTTTATTTGAGGTTTATGCTGAGGATATGCTAGATGGCAATTGCAAAATTTATATACATGCTAAAGGACGTCCAAAAGACCCAGATTGAAAAAGAAAGACTGATAAAAAATCTTTAGAAATTGAACGATATGTAAAGAAATTTATTAAGTTTTTAAAGAGAGAACAGAAAAAATAAAAACAAAATACCAACAGTTAATTCTTAAAAAACGCGCTGTTGGTATTTTTATTCAACGCAAATAAAGGAATAAAAAAGCCATCTACCCAGAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTTCAACACAAGTCACTCTGCCTGCTCCCACTAAATAAATTGACAGGGTTTTATAAAAAAGCCAAAAGCTTTTAAAGTAACTATTTATTTGCAAATTAGTGCAAATATTCGTGTGCTATTTAAATTAACTATAGCACAATTAATAACAGTTGCATGATTGATCAAAGTAAGTTAATTGAAAGGTGAAAAACTACCTTTGAAACAGCACAAAATCCTACTGAATTATTAGCTTTTAAAAACAGCTTCCGCAATGCTGATTTAAAGCCTTTACTTAGTCAAATTAAGGAAACCACGGACATCGAAACTAAGCGCCATTTAGGCCAACTTTACAAGCAATTGGAAAGCACCTTGCAAACACTCCATGATACACAGCTACAAGTCTTTACCCAAGCCCAGTCTAGCAGTGTATTAACTCATGGTGATGTGATGTTGCTAGCAACCTCTTTTGCCCCTGGTTCTAGCAACATTATTTACCAGGTAATTGATGAGTTAGTGAACTATTTTAAGAAGTTCTTGTTTACGGTTAACTATGACAGTGAATTGACTACTATTGCAGACTGTTTTGACCTGCTAAACATTCCCAAAGACCATCCTAGTCGTAACCTTACTGACACCTTTTACTTAGACAAAAACCGCTTACTGAGAACACATTGTACTGCTGCTACTTTACGTGCTGTTAAGGAAACGAAAAAGAGTAATAACCCCGATATTCGGATTGCTTCCTTTGGTGCTGTTTTTCGTAAAGATGATGACGATGCCACCCACTCACACCAGTTCAACCAACTGGACTTCATGTGGATTAAAAAGGATTTTTCTTTAACTAACCTCAAGTGGTTTATGCAGAATATGATTAACCACATCTTTGGCGAAAATACTTCAGCGCGTTTTCGTCTGTCCCACTTCCCTTTTACCGAACCTTCCTTTGAAATTGACATCCGTTGCTGGTTGTGTCAGAATGGTTGTGGTGTATGTAAGAAAACACGTTGGATTGAGGTATTAGGGGCGGGTATCCTCCACCCTCAAGTGATGGCTAACATGGGCTTTAGTGACACGGATAATATAAGGGGTATAGCAGCTGGCATTGGGATTGAGCGGTTGGTAATGTTGAAACACGGCATTAGTGACATCCGTGACCTTTACGACAATAACTTCAAATTTTTAGCCCAGTTTACTGATTAGCGATGTTAATTTCTAAAAAAACTTTAGCTGTTTTAATTCCCGAAATTACCCACGTTTCCAACAATGAAATTTGTGAAAAGTTACAGCAAATCGGGATTGAAGTGGAAGCAATTCGCGCCTTTAAAAACCCTGATTACTTACAGTTGGGTATTCTCCGCGCTGTTACCCCCCACCCCCATGACAACCACTTGTATGTGTGTCAAGTTCAAATAGACAAAAACAAACAGCTCAATGTGGTGACTGGGGCAGTTAACATAGTTGATCCCAATAATCTCAATAAACACGTGATTGTCGCCAAAAAAGGGGCAGAACTGCTCAATGGTTTAATCATTAAAACGAAGAACATTAAGGGAATTATCTCGGACGGGATGTTGTGTAGTTATGTTGATATTAATCCGTTTAGTAAGCATTTAATTGCTGACGGTGATGACACGCATGCGATTGTGCTAGACAACATTAACCGCGATGAATTTGGTGATTATTTAAGTTTTTTAAACCTCGATGACGTGGTGTTTGAGGTTACTTTACCTACCAACCGTTCTGATTTACAGAGCTTAATCTTCCTCGCTAAGGAACTAGCAGCAGTGTTGAAACGTCCGATCTTCTTGGAACAGAAAACCACCATGACCCTGCGCGAGTTCTACCGCTTTCCGCTTAACTTAAGAAACCGTGCTCAGGCCAATTTCTTTGGTGGACTTTTTTTGCGTGATGTAGCAATTACCAGTTCCCCTTGAACTACTAAGGGTTTGTTAATTAACCAGGAGTTACGTCCAGTCAACTGCTTTGTGGACCAGGCTAATATGGTAACTGTTTACACTGGTCAACCAATCCACTGCCATGATGCTGACAAGATCCATGGAAGTGTGGAACTTAAACTAGCCACCCAATTGGAAACGATGTTGGCCTTAGACAACAAGGAGTATGAGATTAAACCGGGTGATCTAGTGGTAGCTGATGAACAGGGCACAATTGCCATAGTCGGTATTATTGGTAGTAAGCGCACTATGGTCGACAATACGACCAACAACATCTTTTTTGAAGTTGTTAACTATAACCATGAGCGAATTAAGCAAACCGCTCAGCGTTTAGGAGTTGCTAACTTTGCTAGTAGGTTAATGAGTAAACCAATTAGTTTACAAGCTACCGAAAACTGCTTGAACTATTTGCAAAATAACTTCCTCAATCCCGAAAGCATTGGCAAGATTAGTAAGTTTTCCAGCACCATTAAAGCCCCAGCTTTTAACCGCAAAATTTACCTCAACTTTAATCAGTTACGGGAGTTAATTGGAGTGACTAAAAAGCAGTTAAATGACTACATGATCCGTAACTACCTTACTAGTTTAGGGTTCAAAATGGAAAACCAAATTGCCCGCGCCCCCGCTTACCGGCAAGACATTACGGTTTGACAAGACATTAGTGAAGAGTTGTTAAAGATCTTGGACCTCAACAAGATTAAAGAGGATGAAATTTTAAGCACCACTAAACTAGAGAAACACGAAAAACTCAATGCTTACGATGCGTTGCAAAAGCTACGCACTAAACTGCAAACCTTGGGCTTTCACAATGTGATTACTTACCAGTTAATTTCACCAGAACGAGCGCGTAACTTTAACCTGTTTGGGTTGAGTAATTTGTGGGAAATTAAAAACCCGCTTTCCAACGAACGGAGTGTTTTACGGGTTGGTTTAATTGACTCCTTGTTGCGAGTAATCCAAAAGAATGCCGCATACAAAAATAAGTTAGGCAACATCTTTGAGTTTTCTTTTGTCAAAACTAAGGACAGTAACCAACTCCACATTGCTGCTCTTTGATTGGAAAAAATGTTTGGTTCTACCTACCAAAAGGATCAAGGCGTTAGTGTCGATATTCCCGCCATGAAGGGCTTAGCGCAGTTAATTATTTCCAACTTTGGTTTTAACTGCGACTTTGAACCAATTACTGAAGGTGAGTACTTCACTAAGAACGTCGGTTTAAAACTAGTGGTGTTTAACGAACAGATTGGTTATGTTGGTTTAATTAAGGATGAGCTGTTGGCGCCTTATGATTTAAAGGGTAGACCGGTGTATGGTTTGGAAATTAACCTAGACCGCTTGTTGAACTCCTTAAACCGGTTGGAGCGCAGTTATACCCCGATTAGCAAACTACAAGATGTCTTTAAGGATATTACCTTTAGCTTCCCCCGTGATGAAAGTCACTTTGAAACGTTTGTGAAAGCGATTAAAAAACTACAAACGATTTTTAAATGGGAATTGATCTCGGTGTTTGACACCGAAAAGGATGGTGTACCAATCACCAAATATACAGTGCGCTATTACTTAAAGAATTTCACTAATACACCATTAACGTTAGAACAAATTAAAGCAGTGGAAACGCAGTTAAAACAACAGTGTGAACTAGCTAAAATAGCACTCGATCTTTAGAAAACGTACTTTTTAAAAGATTTCGTTAGGTCTGTTCGGTTTTCGGTGTCTTCTTTTATTAAACTGATTCGGTTTCTATTTTTAAAGTAATAGAAATAAAGCTAGCCAAACAATGACAAACCAAAACCTACAAGACTTTCTGGACGGAGTTTTGTTAAAAGAGGAAAATTTAAACAAAATTACTGAAGTTTCCCTAGAAAAGGGATTACCGCACCTTTTAGATGAGGGTATCCATAACGAAGATAACTTTCAGATCTTTACGAGCCAAAAGATTGTGGCAGACATGATCAAAATGGTCGGTGTAAAGGAAATGAAAAATGTCCACACTACTGTTTTAGAACCAACTAGTGGTGATGGTGCTTTTACTTGTGCTATTTTGGATTTACGTTTAAAAAACATTAAAAAATCAGACAGCTTTTAAAAGAAGCTTTAACCGGTCTATCCACCATTTATTCAGTTGAAATTGACGAAGAGTTGGTTTGGTTACAACGCAATAACCTTTACTCTATCTTTTTAGCCTTCTTAAAAAAGCACGGTATTTTTAACGAAGAGTTAAGTCTCTTAGTGAAAAAAATCTTGATGATTAATGTGAGTTGGGGCGAGTTAATCACAGAAACCAAAGATAACGAGAACTTCGTTTGTAACTACGATTATTTTTTAAGGAATAAAAAGACAAAGAAAAAACCCGCAAAGCATAAACATGAATTTCCAGAGGGTTTATTTTCCAATGGAGAAGGTAAGTGCAAAAGTTTGTTTGTTAATTGGTCATTTGCTGATGTCATTAATGAAATTGCTCATAATTCCAATTTTGACTTTGCCAAAATAGCAAAGATACCTGGTGAAATAATTCAATTTCAAATTAATTAGTATGAGTCAAAATGGCGATAAAGACCACGAATTGATTCAGGGAAAGGTCAAAAGCAAAACTGAAATAGAAGATTCCGTTATATTGGATCAAGACACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGAGAAGCCGAGAAAAACGACTTGCTCAAGTTAATTGATAACGTCGGTAATGATGAAGTTTTTACTACTGTAGAAACTTGTCAAAGAATGCTTGACGATCTCTTTCCTCAAGACCATGAAGTTTGATCGAATCCGGACTTAAAGTGATTAAATCCTTGTGATAAAAATGGTGTTTTCTTTCGTGAAATTGCGTTAAGGCTAGACAAAGGCTTGGCCAAAGTTATACCGGATGAATATGAACGCAAGAAACACATCATGACCAAGATGCTTTTTAGTATTGACTTAACTAAATTTACTAGCTTGATGGTTCGTCGTACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAACTAGTGAAGATGAGGGCTGTGCAATTGCAAATGGCGCATGATTTAATAACGAATGCGGTAATGTTGTTAAACCTTACAAGGAACATTATTTCGACAAACAAGGAAAAAATAAAAAATGTAAGTTTTGTAGAACAGATGAGAAATTTAAATACCAACAATCTTCTTCAAATGAAAAATACGCCTATGACTTCATTCATTTAAATCCAAACGAATATGAAAGCTACTTTAAAACTAATTTTGGTGTTATGAAATTTGATGTAATTATTGGTAATCCACCTTATCAGTTAGCAAATAATCGTGGTGGTGATGCTGGTGATGGTAATGGAGCAAACCCCATTTTTCAAGAATTTATCTTAAGAGCACTTGAATTACAACCAAAATATTTAGCAATGATTGTTCCTGCAAGATGAATTAATTCCTCTGAAAAAGTTTTTCTAAAGTTCAGAGCAAAATTACAAAAATCAAATGGTTTTAAAGGAATTAATATTTTCTTTGACTCTAAACATTGCTTTCCAAACAGACAAATAAAAGGTGGTGTTTGTTACTTTAATTGACAGAATGGCTATGAGGGTCAAACACTCATAACCACTAAATCTTCAAAAAAAGGCAAACAAGAAGAAATTGAAACTTATAAGAGGGACTTTTTTGCACCTGTTTTAAACAATACTGAGCAAGTTATTTTCCGCAAAAAATTTAACACGACATTTATAAAAAGGTAGATTCTTTTCACGCTAATGAATTAATCCATGCTCTCAGAGAGAGAGAGAGCAACTTAGAAGATCAGCCTTCTAATGGAATAACACACACACAGAGAGAGAGAGAGAGAGCAATTTAGAAACTAATTTCGAAAGTTTCTCTAAACGTGTATCTGGCAAGGATCTGTTTAATTTAGCAACTAATTTTTTTGATAAGTTTCCCTTAGTTGTAAAAGACAAAAAATTTCCTGGTGCAATTTCCATTTATAGCAGTTCGGGCGTAAAATACGCCGAACTGAAAGAGATCCCTAATATAAAGAATAAGGAACAAATTTCCAAATACAAGATCATTATGCCCAGAATAACTGGTAGCGGTGTGTTTGGTGATTACAGACCCAATATAAAAATTGTTGAACCTACTGGAGTTTGCACTCATAGTTATGTAGTTGCTTATTTAACGACAAATAAGTTTGAAGTTGAAAATGTCTTTTTATACATGAAAACTAAATTTTTTAGATTTTTTGTCGAGATTTTTAAAGCAACCATCAATATCAGTTCACATAACTTCAAATACGTTCCCATTCAAGATTTTTCCCGTCCTTGAAATGACAGGGAGCTTTATCAAAAATACAACTTAACTCAAGAAGAATGACAATACATCGAGAACAATATTAGTTCTTATGAAAATTAGCTTTAAAATTAAGGAACATGAAATTTAAGCTTAATTTTCACGAAAAAATCAACCAAAAAGATTGCTGACAATCTCTAATTGATCACAAGGAACGTTCCTACTCATTAGACTTCGTTAATAATACGGAAAAGGAACTACCACTTATCTATGGATACGAAGTAAAAGACTTTGAAAATCACGGGGTTAAAATTGGTTATACAACATGTAAACCGTCTGAGAAAATTCAATCAGCGATAGAAGAACGTATCCTTTCACAAGAAAAAGAATTTCGCTTTTTAGATGAAAATATAGAAAAGATAGAGGAAGTTAAAGTTATCTTTTGAGCTATTGCCATCAATGAAAAGGATGAATCATTCAAGGATTATTCACTCCATTCATTTATTAAGGAAAAAAACTTATTAAAAGAATCGCAAGCTGGCGGTGAATGGTTCATAGTTGATGAAAATAAGGATAAGTTTGAATATCTTAGCCAAATTTTTAGACAGTTTCGCGCACCTTTTCTTTTTAAAAATAAGGAAATTTATACACCTAGAAAAGAGCAAGCCGCATGTGTTGAAGTGTTAGAAAGATACTTTCAAGCCAATCCCGAAAACGGTAGGTTTTTAATGAACTGCAAAATGCGGTTCGGTAAATGTTTCACCTTGTACTCTTATGCACAAAAGAATAACATTAACAAGATTTTGATCCTAACGTTTGTTCCAGCTGTCGAAGAATCTTGAAAGGATGATTTAAATCACATTGAAAAGAATTACAAATTTTATACCGATGATGATTTGCAAAAATCTAATTTTGATTTAAAGAATCAAAACGAGCCTTATGTAGTCTTTTTATCACTGCAAAACGTCTTAGGTAAACAAAGAATAGATGGCGCCAAAACTGATTTTGATAAAGAGCGAATTAGTAAGCTACAAGAGATTGATTTTGATTTAATTGTTTTTGATGAGTACCACTACGGAGCCAACAAAAAACGAACCCAAATCAAAGTTGAAAAAGTCAATAAAAAGATTGATAATCCAGAACAGCAAGATAATCAAGATGATGCTGAGGAAGAATTGGCAAGTACTTTTAAGTTAAAAGACATCAAAAGTAAATTTCAATTTTCATACAAACAATTAGTTTGTCTGTCAGGTACTCCTTTTTCCTCTTTAAGAAATAATGAGTTTTCCAGTAAAGATCAGGTGTTTACTTATTCTTACTTTGATGAACAGAAGGCCAAATCAGCAGAAAATCACCCACTCAAATTAGGGCAATACGGAATATTTCCGGAAATGAATATTTACTGTTTTGAATTGGCGGAAATTTTTACTGCACAAGAACAAGAAATATTCATAACTCCAGGTAAAGGTAAAAATAAGTTACCTGAAATTAGTTTTCGAAAGCTGTTTCAAACGGAAAACGTTTCTAAAGAATCAAGCAAACCAGTTTACAGATTTGTAAATGAAAACCTGGTTGAAAAGCTTATAGATTCTTTAATTGATAAACGTAAAGGTTTTAGCCACACACCTTTAAGTTGGGAAAACATTGACAAGCATAAGCATTCCTTATTAATTTTGCCAACTAGAGTTGCTTGTTTTGCGTTGGCCAATTTACTTAAAAATCACTGGTACTTTGAAAACAATGATTTTCAAATTATCAACATGTCAGAGAGCCAGTTTGGTAATGGCAAAAAAGCTTTAATTGAGCTTAATAAACACTTAGATGAAGCTAAAAAGACTAATAAAAATACTTTAACTATTACTGTAGCTAAATTAACTATTGGTATTACAGTTAAAGAGTGATCGACAGTATTTTTCTTGAAGGATTTAAAGGGTGCTGAATCTTATTTCCAAACGATTTTTAGAATCCAAACTCCATATATAAAGAACTGTAAAAATCTGAAAGAAATTTGTTATGTTTACGACTTCAACATGTATAGGTGTTTGGAAGTTACTAACGAGTATTCAAAACAAACACAAACAGACCCTAAATTTAGTGCTAGTTGATTCCAAAACTTAGACAAATTTTTACCTATCTATTTAGTTAGAGGCGATGAAATTCAAAAGACAGATCCAGAAATATTGCAGAAGTATGAGTACTTCATTATGGACAAGCGTGCATTCTCTACAAGATGAATGGATGAAAGTAACATTATCGACATAGATGTACTTTGCAATGTTGGGCAAGATGAAGATGCGCAAAAAATATTGAAAAAAATATTAGCTCATAAAAAATTTAAATCATCCAAAAAGAAGCGAGAGTTTGAAGACGTTCATTTGGAAAAAAGTCCAAAAAGTGAGGCATTTAGTGAAGGCGTTCGCTCTGGTAAGGATTACGCCGCTGAACAAGGTAATGTTTTAGAAGAACTTGAAAATTTCTGAAACTTTAACCAAGCATTAGAACAAAAAGCAAAAGCTGAATTTCAACAAAAAAACTTTGATGATAATGAATGAAACAATTACAAAAAAGGGTTTAATTTTGGAGTTAATAAGCATTTTGAAGATAAAGTTTCCATAAAGAAAATTGTTCAGAATAAAATCAAAGACTTTAAAAAAAGAAAAGGAAGAACAACTTCAACTTTTAAAAAATGAAAATGGTTATATTTCTGATGAGAATAACTTAAAAAAGCTAAGAGATTTAATTAAAGAATATGTACAAAACAGAAGCAACATACCTCCAGAATTAGACTGATTTTTTTACCGAGACCGTTTTATTCAGCTCTTTGAAAAGAGTTGTTACCAGCATTTCAAAAAGAAGACTTTAACTGAAGAACAAAAAGAGAAAAAGAAAAAAGTTAATGACAACTTTGATAGTTTAATGCATACATGGGCACGTGTTCTGCACTTTCTTTATGTATCTGTTTATAGAGAAAAAACTTTAAGAGATGTGTTGAATAAAACTGATCCCAAAGTTTTTTCAGTGGCAGTCGGAATTGAAATGGATGAGTTTTCCAAAATCCAAAAATTTTTTAAAGTGGAAAATATGAATAGACTCATCGAACAGTTTAGTTTTAGAGAAAGTGTTAAAACTGAAAACACAGAAAAATCACAGTATAGAAACAGTTTCAATTGATTCGGTTTTGGCCCCGATAGTTTAAAGAGCTAAATTTCACATTTTTAGTATGAACAAAAATAGTGGTAAAGACTACGAATCGATTCAAGGTAAGATTAAGAGTAAAGCTGAAATAGAAGATTCAGTTATATTGGAAAAAGAAACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGAGAGGCCGAGAAAAATGACTTACTCAAGTTAATTGACAACGTCGGTAATGACGAAGTTTTCACTCCCGTAGAAACTTGTCAAAGAATGCTCGATGAGCTCTTTCCTGAAGATCATGAAGTTTGATCAAATCCCGATCTCAAGTGATTAAATCCGTGTGATAAAAACGGAGTTTTCTTTCGCGAAATTGCGCTAAGACTTGATAAAGGATTAACCAAAATTATTCCCGACGAATATGCCCGCAAGAAGCATATTATGACCAAGATGCTTTTTAGCATTGGCTTAACTAAATTTACTAGTTTGATGGTGCGTCGCACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAATCAGCGAAGATGAGGGTTGTGCAATTGCAAATGGCGCACGATTTAATAATGAATTTGGCAATGTTGTTACCCCTTATAAAGAACACTATTTTGGAAAAGAATCGAAAAGCAAAAACTGTCACTTTTGTGGGACAAATAAGAATTCTAAATATGTAAATTCTATGACTGAAGAAAAGTATGCCTATGACTTCATTCATTTAAATTCGGATGAATATGAAAACTACTTTAAAACTAATTTTGGTGTTATGAAATTTGATGTAATTATTGGAAATCCGCCTTACCAGCTATCTAATGGTAGCGGTTCTGATGGCAATGGAGCCAAAGCAATTTTTCAAGATTTTGTTTTAAAAGCAATTGACTTAGAACCAAAATATTTAGCGATGATTATCCCAGCAAAATGAATGATTTCTGCTGAAAATATTTTTTTAAATCTAAGAGATAAATTAAAGAAAAATAAAGGTATAAAAGAAATTAATATTTTTTTCGACTCTAAAGACTGTTTTCCAAAGAGAGAAATAAAAGGTGGCATTTGTTACTTTATCTGGCAGAATAACTATCAAGGTAAGACTCTTATAAATACCAAATTTTCAAAGAAAGGAAAAAGCACAGAGATCGATAGTTCTAAAAGAAACTTATTTGTTTCCGTTTTAAACAACACTGAACAAATCATTTTTCGCAAAAGGATTTGACAAGACATTTACACAAAGATTGATTCCATCTCTGCTAGTGAAATGAAGAACACACACACACAGAGAGAGAGAGCAATTTAGAAGTTAGATTTCTTAATTTCTCTGATTACGTTTCAACGCAAAAATTCTTTAATCTAAGAAGTAATTTTTTCGATAAATTTCCTTCGATTTTGAAAGCAGAAAAATTTCCCGGTTTGATTGCAATTTATGGAAGTCATTCGGTTATTAAACACGTGGATATTAAAGATATTCCGCAAATAAAAAATAATGAAGAAATTGCTAAATACAAAATTATTGTTCCACACGTTTATAGCCGTGGCAATGGAACTTTTGGAAATTACAAACCAAAAGTAAAAATTATTAAACCTAATGAAATTTGCACCGAAACTTATTTAGTGGTATATCCCACCGAAAGTAAAGTTGAGATTGAAAACGTCGCTTCTTATATGAGAACTAAATTTTTCAGATTTTTAGTTGAAATTTTTAAGGATTCTATTAACACAAATTCTCAAAATTTCAAGTTTATTCCTCTACAAGATTTTTCACGTCCTTGGAACGATAGAGAACTTTATGAAAAATACGGTTTAACACTCGAAGAACAGCAATACATCGAGGCGAACATAAGTGCTTACGAGGACTAAATTGCCGCTAATTGAATTGATTAAAGTTTTGTTTTTAAGCGTTTTAAGTACTACTTTTAATACTTCGAAAAACGCAATCAAAACAAATACTCCCAAAACTAAATTTTAGATACAAAAATAACAACTAAACACATTAAAAAGTGGTTTGCTATACTCTTTTATTACTAGTGGTTTTACACATTTTTTCACTTAACAAAAAGTTTTAAAAAACACCAAAATTGCCACCAGCTAGCAAATCCAACACATAAAGAAAAACGTTCTTTTAAGGCTTATTAGAATAAGGGTAGATTATTCATTAGTTGTTGTATGCTAGATAAATTACTCCAGAAATTTCGAGACCAAAAGAAGCCCGTCTTCCACAAAGAAGAGGGCTACTGGGAAATTAGTGCCTTGCGGAAATGGGCAGCCATTTTAATAATTGCCTTTGGAGCTGGCATTATTTACATTGTGCCCTACTTTGCCTTCTTTCAGTTTAAGACAGCAGTAGCTAATGTAACGGGAGTGGAGCCCAACAGAATCTCCCTTCTCTTAACTGCTTACGGGATTGTTTCATTGCTGTTTTATATTCCTGGTGGTTGGTTAGCTGACCGGATTTCAGCTAAAGCGCTGTTTTCGGTATCAATGTTTGGTACTGGCATTATTACCTTCTGGTACTTCTTAGTTGGTTTGAAGGGCATAGTGTGGATTACACCCAACTAGTTATTATCCACGTCCTGTATGCCCTTAGTACGGTCTTAACCTTCTGGAGTGCCTTCATTAAAAGTATTAAGTTATTGGGTCCAGCGGATGAACAAGCGAGGTTGTATTCCACATCTGAAGGATCACGCTACTTAATCCAAGTAATTACTGGTTACATTAGTGCTGGTTTGGGTGCTATTGTCCTTATTAGTACTCCCTTTCTCATTTACAAAGCACCAACTGCTGCTGACCCTAAAAACTTAGCTTCTTCCTCAGGGATCTTCTTTGCCTTACTGTTTTATGCCATTTGTTATGTGTTAGCTGGTTTACTGTCACTGTTTTTTTTACCAGGAAAGTTCTGGGAAAAGCGCAAGATTTACTTGGAAGATGAACGAATTGAATACTTTAACCCTGTGGACCAGGCTTATCACATCTTTAAATCGGAAGCAGAATTAGAAGCCTTTAAGAAACATAACACTAAGGTTTTTTGAACGAAGGTATGAACTGATACCAAAGAAACTTTGAGTTCTACAAGCGTTTGGTTAATTGCCTTTCTGATCTTCTTTATTATGAATGTGTACCAAACGATTGCTAACTTTGGTACTAACATTTTGGAATCGCTAGGACTAGAGGCAGCACTCTCCCCAACTTTAAGTAATACCTATATCTATGGGATTCCGGTGTTAGGGGCAATCTTTTCTGGTTTGATTAACAAAAAGTTAACTAAATCAACCGCTAAAAGTCTGATTGTGCAAGCCTTGTTTGTCATTTTGGGTACCTTAGCCTTTTTAGTAATAACACTAGCTTCCCCAGCCAAACCAGAAACTGGTGCATTTAACCAAGCTACCTTGTGAATAGCATTTGTGGTGATTTGCTTCATCATGTTCTTTATTGGCGCGAACCGTTCCATTTTTTGGAGTACGATCACAGAACTGAAAGTCAATAAGGAAATAGTAGGACTGGCAGTTGGTTTTATTTCGATTATTGGTTTTTCCAAAGATGTGTGGTTAAGTCCCTTACTGACTGGTACTACTAACCAGTTCATTGTCAAAAACAGTCAGGGTACCTCGTTTTACAGCCAGCAAGCCTTGGTAGCCTGAGCCATTTTTGCCCTAATTAATGCTTGTTTAGCACTACTAGTGACTTACATGATAGTGCGCAAAGTGAAGTATGGCAAAGTGTGAGTTAACCCTAAATTTAAAAAAGTCTTTGCACTAGGAGAACAACAATATGGACACTAAATTAACACCACCCTGGTTGCGTCAAGAAAATAACCTGCTCAACCCCGAAGTTACCCAACGTTTGTTTATCAACCAGGACAACATTCCGAACTTACCCACTGAAGCCCCCCAAGTTTATTTAGCCCGTTTTTTGGAATGAGTTGAACCACTAGTCTCGAAAGTAAAATTTGTGAAAGCACAAGCAGCAGTTCAGGATTACCTCAATTCTAAAGCCTGTGCACAAATTGAAACCATTATTGCAGAACGGGCGCAAAACACCCATTCGAGTTGGTTAGCTAACTGGTGGGTGCAGTACGCTTATTTAACTTCGACTGGCCCGGTTAGTCCGGAAGTTAATGCACCCTATTATTTAGAGCTCCCGACTGTTGGTTGAAGTCAAGCCGAGTTAGCAGCAGCATTGTCAGCCCAGCTATGACACATATACCAGCAAGTCCAAAAACGGCAGTTAACGAGCTTTAGTGTCAAGGACAAGCTGTTTTCACTCGATACCTTGCAATCAATCTTTGCTAGTTGCCAGATTCACCGCGCTGATGGGGATGTGTACTTTGTTAATGATCAACCTGCTAACTTCATTGTGGTGATTAAGAACAATGTCTTCTATAAGTTAGTAATAGATAACAGTGGTAGCTTAGAACAACTGCAAGCGCAACTGCAGTTAAGCTTTGTGCAAATCTTAGACAATGAGCTAAGTCACCCACCGCACTGGAACTTACTCACTGCTACCACAACCAAAGCGGAGTCACAAAGTTTACTCGATCAGTTGTGAGCGCAAAACCCAGAAATGTTGTTGGACATCTATAACAGTGCCTTTATTGTGAACTTGGACAACGTCGAATTAACGACTCCCTTACAACTGTTGCGCAATTCGACATGAACCCCTAACTTTAACCGTTGGCACGCTAAGGGGATTCAGTTGGTCATTACTAAGAACGCTCAGTTAGTCATTCTAGCGGATCACACTAGTTTTGACGGTTCTAGTGTAGCTACCCTAGCCAACATCTTTGTCTCTAAACTCCAAAAGGTCAACACTGAGGGTGCCAGCGCTTTAACACCGACCATGCTTTCCTTTCCAACAGTAGACCAGGATAAGCAAAAGTATTTTAAGCAATTGAGCAAAAATTTTAAGGACTATGTGTACAATGCCGTTATGTTTGAACTGAAATGGGACTGGTTTACGAAGCCACTTATCAAAGCCAAAGGGATTAAGAACTCCGAAGCCTTTATCCATCTCTGTTACCAAATTGCCCAGTACCAAACGAACAAAAAGTTGCAGAACACCTATGTGGCCGTGGACATGCGTCAGTACTTCCGGGGACGCACCGAGTGTCTTCGTCCTTTAAGTAAACAATCAGTTGCCTTTGTCAAACGCTACTGCAAAGATCCGAAAGGTACCTTAAAGCAGTTCCGAAAGTACTACCCAGCGATTGAAAGTCTCCACTTTGAAAAAACCCGGTTAGCGCAAAAGGGTAGTGGGGTCAACCGTCACTTGCTGGGAGCATATTTGGCTTGGAATGAACACCAAGACACAATAGCTAAACCCGCTTTATTTGAAACAAAGGCTTGAAAAACGATTGCTGCCAACCCACTGTCCACATCGAGCATTGTGGACAAGTATTTGCGCAACTTTTCCTTCGACCCCGTAGAGCCAAACGGCATTGGTATTGCCTATGCCATTGATGACACTAATTTCCGTGCCATTCTCAGTGTGTATCAGCACAACTTACAGTACCTCAAAGATTGGATGAAACACTTTGAACAAACAGTGAAAACAATCCTTAAAACTCTTAAATAAAATTACTCCTTAATGGTTAATTGAGTTAAAACGGTTGGAGATTGTTAAAATATTGGCGCTATACAAAGTAGTCTTTACGACTCGCCCGAATGCCGTTGATTCTTGATCAGGGCTGTTTTGTTAGTGAAAGTAGATTTCATAGATTGGCACAGAACATAAAACAAGGTTCCAGGCGTGAGCGCGAACAAAAACCGTTAATTAACGATAAAATTGGCTTTAATGAGTTCATTTTAATTGACGAAAATGGCTCCAATTTGGGGACGGTTAGAAGGACCGATGCCCTCAAGATGGCTGAGGAAAAACAACTCGATTTAGTCTTAATTGGTTCAAACCCAGCCAAACCCATTGTTAAGCTCTTAGACTTTGGTCGTTACACTTATGATTTAAAACGCAAAAAACGCCAGTCCAAGAAAAACCAAACCATTATCCAAATAAAGGAAGTGGTAGTGAAACCGACAATCGCTAAACATGATCTGGAATTTAAAGCTAAGCAAACGACGGGATGAGCTGAAAAGGGTTATCACGTTAAGTTTGTAGTGCGCGCTTTTGGTAGAGTAAGTACTAGGATTGAGTTAATTGAAAAAGTCTTTAATGACTTTTATCTGCTAGTAGAACCAGCAGTGGAGGTCCAAAAACCACTAACTGCTTCTTCCAAAACAATGTATTCTGCTTTGTTAATTCCCCGTAAGAAATAAAAATTATGAAAGTAAAAAGTGCTGCAAAGAAGCGTTTTAAGCTCACTAAGTCTGGCCAAATTAAGCGCAAACACGCTTATACTTCCCACTTAGCACCGCACAAAACGACGAAGCAAAAGCGCCACTTGCGCAAGCAAGGAACTGTCAGTGCGAGTGACTTTAAACGCATTGGTAATTTAATTTAAGGAGTGAACTATGAGAATTAAAGGTGGAAAACAAACCCGCGTACGTCGCAAGAAGTGGTTAAAGCAAGCTAGTGGTAGCTTTGGTACCCGACATGCCTCTTATAAGGTGGCAAAGCAAACGGTGATCCAAGCCGCTAAGTATGCTTACCGCGATCGTCGTAACAAAAAGCGCGATTTCCGTTCGTTGTGGATCTTGCGCTTAAACGCCGCTTTGCGCGAACAGGGCATGACCTATTCGGTCTTTATCAATCTATTGAAGAAGCACAACATTGAGATTAACCGCAAAGTGTTGTCGGAACTAGCCATTAAAGAACCAAGTAAATTTAACCTAATTGTGCAAAAGGTTAAAAGTGAACAACCGAAAGCCGCTAAGCCAGCTGCTTTAGGAAACTAGCGACATTCTTAAAGGTAGTTTTACAGACCGCACTGTACTGGGCGAACTTGTGAAACTGCCTTTTTGTTTTTAGTAAGTGTAACGCTTCCACAATGCCATGGGCACTACCCTTGGGAAACTGTACACCGTACTGGTGGCTTAACTGTTCCATTTGTGCAATAAAAGCAGCACGTGCTAAGATCGAAGCAGCGGCAATTACCTTAGAATAGCTCTCACCCCTAATTAAGAAGTGGTTGGGAATAACTAATTCCTTTAACTCATTTTGCAGGCTGGTGAGTTTTTGCGTGTATTGGGTGAACAGTTTGACATTAGCAAACTCATCAATGCTAATGTCACATTGTTGTGCTGTTAGTTTGTTTTGTTGCAACAACTGGTGGTACACCTTGCAGTGCAAAAAGGTCAACATTACGTTGACATTTTTAAAGCTTTGCACTAGTTGGTTGTAAGTTTTAGCATCAACGCTTAATGTAACTTGGTCATGAACCGCTGCCTTGATTTTAGGCACTAAAGCTTTAATTTGGTAATCGTTAAATTGCTTCGAATCCCCCACACCAATTTGGTGGAGTGTATTGATTTTGTCCTTATGAATTAAAACAGCACTTACAGCAATTCCGCCAAAGCTATCACCCTTGCCAGATTCATCACTGCCAATTAGGTAAAGTGCGGTTGTGTTTGCGTGTTGCAATGGCTTATAAATTAATAAAATTATACCAACTCTACTTTCTTAGAATAAACGTTACATGGCAGAGGCAAAACGTGATTATTATGAAGTCTTAGGGCTTAGTCGGGATGCTGATGATAACGACATAAAAAAGGCCTTTCGTAAACTAGCCAAAAAGTACCACCCCGACCGCAATAAGGCACCGGATGCGGCTCAAATTTTTGCCGAAATTAACGAGGCCAATGACGTGTTGTCTAACCCCAAAAAACGTGCTAACTATGACAAATACGGCCATGATGGGGTGGACAATGAAGGTGGTTTTGCCTTTCAAGCTGATGTGTTTGATTCTTTTTTTGAGGAGATTGAAAAATCGGGTGCCTTTGATAATTTAAGTGAGTCTAACACCAAAAAGAAAGAGAAGACTAAAACTAAGAAGAAGGGTTGGTTTTGGGGCAAATCCAAACAGGAAGAATCTACCAGCGATACTACTGAATATGCCGATGTTGACGCTGGTTTAGAAGATTATCCGCCTCAATCTGATTATCCAGATGATATTCCTGATGTGGATGCCAGGATCGAAGAAGTGGATCAATCTGCCTATGCCGATGACATTCCCGATGTAGATGCGGGAATGGATTGGGAACAAAACGCAGAAGTAGCAAATTCTGCTAGTGAAATCATCCCCGATGTCGATGCTGGTTTAGCGGATGAGTTTAATACTAGTAGTGCAGCACCCCAAGCTAGTGACTGGGAAGCAATGATTGGTAATCCTGAGTACGGTTACTTTGATGCTGCTGGTGAGTGAAACTGAAAGGGCTTCTTTGATGAAGCGGGTCAATGGGTTTGACTAGAAGAGACTGAACCTAGTAGTGTCAGTAATGACGAAACTACGACAGATAGTGATGCAGTGACTGCTGCAACTACAGTAGAGGAAACGGATCAAGACAGTTGAACAGCAAACTCAGCACCAGAGTCAGTTGATGTAGAAACTCCAGTAGAATTGCAACCGGAAACTGAACCAGAACCAATTATTACCTTAAGTTCAGAGCCAGTGGAAGCACCAGCTTCTGTTGTGATTGAACCTACCCCTGAAACTGAGGAAACAACTAGTGCTGTAGAAATGGATGCTTCAGTTAAAGCAGATGTTTCTGATGAAGCTGACGCAACTAATGAACCAACTGAACAGGATACCATTTCAGAGCCAGAACAAGAAACAGACGCGGCTGCATTAGAAGAAATTAACCACACCACTGCTGATTTGGAACCAGCAGAAGTTAGTGCTACTAATGATCTAGAACAAGATGTAGTAGAGAAAGTTAATTTTTCAGAACCAGAATCAACAGTAGATACTGCTGCAACCGATCCTGTTGTAGAACAAGCTACGGAAACCTCCACCAATGGTTTCAAATTCTTTAACTTTAGTTCGTTTGTTTTAAGTGACCAAAACCCCAATCCACAAACACCCACCCACCATGAAGAAGATGCTGCAGCCCCCGAGCCAACCGTTGATGAAACTTCTGGAGAATCCACAGCCCCCGAAGTTACCATAGCTGAGTCAACTGTAGAATTGGAAACCGCAGCGGAAATTAATAACCCCGCTACCTTTGTGGAAGAATACCTCCAACCAACTAAAACTACGGTTGTTGATAAATTAGATGAACCAACTGTAGCAAAACCTACTGTTAGCGATTCTGAAAATTCAGTTGCACCAGAACCGGAATTTGTTGCTGGACCGGAACAAACCTTTAGCTGAAAACCAGCAATATCAGAAACCGAAGAAATTCCGTTAACTGCGGTAGAACCTGCTTCAGAGACTCAAACACTAATAGCAGAAGATGTAACTTCGCCAGTAACACCAACAGCCACAGCTATTCCCGCACCTTCAATTAACGCTGTACCAACTGCACCAGTAGCGGAAACCTTTGAGGCGGCGGTTGACTTCTTAAAGGAAGCTGCTAAGATTGAGGCACAACTACCACTAGTCCCAACAGTCCCAGAACAGATTGATGGAACTGATCCTAGTTTACTAACCCAGTGAGATGAGTACCTTGAAAAGACCCGCAAGCTGTTCCACAAGCTCTTTTTAACTGAGCAACTGCCTTTTATTGTTAAGACTGATCAATTTGAGATAGTTGACCCCAATTTAGACGAGCACAACGTTAACTTAATTTACACCGAACACGTACCCCAAATTTGTTTCTTGAACGAACAGCTCAAGGAAATTAGGTACACCCGTAAGTTAGTCGATCCACAAACTCAGGTAACCACCACTGAATCAATTACCCTAGAAGTTCAGCTATCACATAAATCCCAAACGGAAGCAATTGCCATCTTTAAGGGCTTTGGTCATGACTATGGTAGTGGCTGTGGTGACCTTAAGGTAGTACTTAAAGTGATCCCTAGTGTCTTTTTCCAACTGCAAGCTGATGGCTTACACACCGCTGCCCTAGTCGACCCTCTAGTGGCTTACAACGGTGGTTTAATTGATTTCTTTGGTCCAGTTAACAGCTTTAAAGTTGATATTGAAGGTGGGATTGCTAACAACGATTTAATTGAGTTTAACCAGTTAGGAGTACTAAGAACTAAGACCAAACGTGGTAGTTTATACGTCCACTTGTACTATAGTAGTGTAGCCAAAAAGGGCACTAAGACTAACTGCCAAGTGCAACAGTTCTTTGATCTAGTTCATGTGGAATACAAGCTGTTAAACTACAACTTAAAACAACTCCACAACTACCACTCGGCTCTCACTGCCCAGAAAAAAACCTTGGACCGCAAAAGCTATCAATGTTTAGCAGTGGAAAGCCATTAATTAGAATTACAATAAATTACTCATGAGCGAAAACTCACTCACAATTACCGAAATTCTCAGTTCCATCCGTACGCTTTTAGTCAAACACAATAAGGCTAAAGTAACCCAAATTGAAAAGGAACTTTTACAAGCAGTTGCTGAACTGGAAAAGAAGTTTAAGCAACAAGTACAAAACTTCAACGAGTTGCAACAAAAGATTCCTAACTTACAAAAAGTCAACGAGGAATTTCGCTTAAAAGTGGAAAAAATCCAGGAAGAAGCGCAGAAGAAAATTCAGGAAAAAGTGGCAGAACTGACCATTAAAAGTAAGGAAGAGTTGGAAAATGCAAAGAAGTATGTGATCGAAAAGTCGATTGATCAACCCTTAATTATTATTGACCAGTTCGAAATTGCACTTTCCTATGCTCAAAAGGATCCCCAAGTAAAAAACTATACCACTGGGTTTAACATGGTGTTGGATGCCTTTTCGCGCTGATTAGAAGGTTTTGGTGTTACTAAGATTGCCATTGAACCAGGGGCACAATTTGACGAAAAGGTGATGGCAGCACTGGAAGCTGTGCCTTCTGATCAACCGGCTAATACGGTGGTGAAGGTATCGAAATCGGGTTACAAACTCCACGACAAGGTCATTCGCTTTGCTTCCGTAGTGGTAAGCCAAGGTAATAAAACAGAATAATGAGCACAAATAAAAGAAGAACCATTCAGATTGAAATCACTGAAGAACACTTCAAGGATTTAGAAAAAGCCTTGGAAGCCTTGAAGGGTACCCAATTACCCTTCTCCACTACCGTAGAACAGTTTGTGGAATTAATTTTGTCCAACTATGTCGCTACTTCCAACAAGATTTCTAATTTAGCTGAAAGTGGCTTTGATGTGGCTTCTATTCAACAAGAATTAGAAAAAATTGGCAGCGCTGCAGGGGCGGATGATGCGCTCAAAAGCTTCTTGGATGAGTTGTTAAAAACATCGCAAAAAAGCTTTAGCAATACCAAAGATGGCAAAAAAAATGATGACGATAATAATTCGTCATCAAAATCATAAGAGGAAATATTAATAATTGGTGCCGAATAACAGAGTCGAACTGTTCTCTGATCCTTACCATGGATCTGTTTTGCCCCTAAACTAATTCGGCTTCAAGGATAATTCTAGTTTAAATTTGGACGGAGATGAAAAATAATTACAGCGAAGCGAATATCAAAATTCTCAAGGGGCTAGATGCTGTAAAAAAACGCCCGGGAATGTACATCGGCTCAACCGATAGTCGCGGTTTTCACCACTTGCTGTGGGAAATTTTAGACAACTGTGTCGATGAAGTTTTATCGGGCTTTGCTAATACTATTGCCGTTGTTTTACATGCCGAAAACCAGATAACTGTTAGTGACAACGGTCGTGGTATTCCCTTCGAAACACACTCCGATTCCAAGATTTCTACGATTGACACCGTCTTTACCTACCTCCATGCCGGTGGTAAGTTTGATAACGATTCTTATAAGATTGCTGGAGGGTTGCACGGGGTGGGGGCTTCGGTGGTGAATGCCTTGAGTGACCAGTTACAAGTAACGGTTAAACGTCAGGGCAAGGTGTACCGCTCGGTGTATGAAAATGGTGGTAAGATTAAGCAAAAAGCGCATTGCATTGGTAATGCCAAAATTGACGAACACGGTACCAGTGTGACTTTTCGTCCTGACCCAAAGGTCTTTAAAAAGATCCACTTTGACAGCGAACTAATTCGTGCGCGGTTAAAGGAATTAGCCTTCCTTTTCAAAAAGTTACAACTGACCTTCGTTGATGAAACCGGCAGTGGGGAAAAAGAGGTGTTCTTTACCGAAGCCGGTATTAGTCAGTACTTGGACGAACTTAACGCTGATTCCAAACAAATTGCGCAAAAGATCTTTGTTAGTGGCACTGAAGATGACATTGAACTGGAAGCCGTGTTTCAGTTTATTGATGGGGAGGATGAAAAACTACTTTCGTTTGCCAATTCGATTCGCACCAGCGAAGGGGGTAGTCATGAAGCGGGTTTTCGTCAATCTGTTGGTGATGTGATTAACAACTACTGCCGCAAGTACAACTTCCTAAAGGAGCGTGACAAGAACTTTGAGGCCAGTGAAATCCGTGAAGGCTTAAACGGCATTATTAAGGTCAATCTCCCCGAAAAGATTATTGCCTTTGAAGGGCAAACGAAGAGCAAACTGTTCTCCAAGGAAGTTAAAGCGGTAGTGCAAAAGCTAACCCAAAAGCACTTCTTCCAGTTCTTGGAACGTAACAGTGTTGATGCTAAATTAATTGTCGAAAAGCTGTTTTATAACCGCAAACTGCGCCAAGAACTCAAACAACAACGCCAAATTAAAAAGAACCTTTCTAATCCGAAGGCCGAGCGCATTTTGTTTGGTAAATTAGCACCGGCGCAGTCCAAAAAAGTGGCTGAACGCGAACTGTTTGTGGTGGAAGGTGACAGTGCTGGTGGTACGGCTAAAATGGGACGAGATCGCTTTCTACAAGCCATCTTACCGTTACGCGGTAAGGTGTTAAATGTGGAGAAAATTAACAACAAGAAGGAAGCGATTAACAACGAAGAGCTGTTAACACTCATCTTCTGTATCGGTACTGGTATTGGTAACAACTTCACCATTCGCGATCGTAAATACGACAAGATTATTATCATGACTGATGCTGACAATGACGGTGCGCACATCCAGATTTTACTGCTCACCTTCTTCTACCGTTACATGAAACCCCTAATTGAAAAAGGACACATTTACCTAGCACTTCCACCACTTTATAAGTTTGAAGGTCGTGATAAAAAAGCCCGTTATCTCTGAACCGAACAGGAATTGGAACAGTACCGTGCTAAGCATAGCCACTTTAATGTGCAACGGTACAAGGGTTTAGGGGAAATGAATGCTGATCAGCTCTGGGAAACCACGATGAACCCGATGACGCGTAAACTAATTCAGATCAAATTGGATAACTTTATCCAAGCTGAAAAGCAAATTAATGTTTTTATGGGTGATAAAACCGAATTGCGTAAAAGCTGGATTGAAGCTAACATTAACTTTAGTAGTGAGAACTAATGGAAAAGAATAAACAAGCGTTATTACTCCAGGCCATCGAAGATGTCTTTGCCTTTAGCTTTAGTAAGTATGCTAAGTACATTATCCAAGACCGGGCTTTACCTGATCTACGTGATGGGCTTAAACCAGTTCAACGCCGCATCCTCTATGGCATGTACCAAATGGGCTTAAAACCCACTTCACCCTACAAAAAGTCAGCTCGGGCAGTCGGAGAAATTATGGGTAAGTACCACCCCCATGGCGATGCATCGATCTATGATGCGATTGTAAGGATGTCACAAGCCTGAAAGAACAACCTCACTACTATCTCCATTCACGGGAATAATGGTTCCATAGATGGTGATAACGCTGCCGCGATGCGCTATACGGAAGCGCGTTTAAGTCCGTACGGGTTTGAACTACTCAAAGACATTGAAAAGCAGTTAGTACCGTTTGTGAATAACTTTGATGACAGTGAGGTCGAACCGAGCGTGTTACCAACCCTGTTACCTAACCTCTTTATTAACGGTACCAGTGGAATAGCGGCGGGTTATGCCACTAATATTGCACCCCATAATGTCGGTGAACTGCTTGATGGTTTGAGTTACCGGATTGAAAACCCTGATTGTGATTTAAAGGCCATCTTAAAGATTGTTAAGGGACCGGATTTTCCAACTGGTGGATTAGTGTACTTTGAGCAAGAGTTGGCGAACATCTACCAAACTGGGAAGGGTAAGTTTGTGATTCAGGCCAAATATGAAACGAACACTGCGTTTGGCCAAAACCAGATTGTAATCACCGAAATCCCATACGAAACGGTGAAGGCCAATATTGTTAAACAAATTGAAGAGTTAATTAGTGACAATAAACTCAGTGCCTTGGAAAGTGTGATTGACAGCTCTGATCGTAGTGGTATTCGGATCATTATTAACCACAAGGACTTTTTGAGTGCTGACAAGATTATGGCGTTCTTGTTCAAACACACCCAACTCCAAGTAAACTTTAACCTCAACAATACGGTAATTGCCAACCGTTGTCCGGTACGGGTGGGGTTGTTAGCTTACTTTGATCAGTTCCTAGCCTTTGCCCACGAATTGATCATTAACAGCGCCAAGTATGATTTAGCATTGGCCAATAAGCGCTTGGAAATCATTAAGGGCTTAATTAAGGCGGTTTCGATGATTGATGAAATTATTCGTTTAATTCGCCGTGCTACTGACAAACAGGATGCGAAAACCAAGCTCATTGACAAGTATGCTTTTACCCTAAATCAAGCCGAAGCGATTGTGTCGTTGCGTTTGTACCAGTTAACTAACACCGACATTAAGGTCCTGTTTGCCGAACAAAAAGAGTTAGAGCAAACGATCCAAACAGCCGAATGCTTAATTGCTAAACCGCAAGCACGAAACCAGTTGTTACAAGCACAGTTTTTCCAATACAAAAAGCAGTTTAACCAACCACGACGCGCACAAATAGTTGGTTTAATTGAAAAGCAAAAGGTACAAGACAGTGACTTCATCGAACACAAGGAAGTGGGCTTACTCATTAGTCACGATGGCATCTACTTTAAGTTTGAACCAGAACAGTTAGCGAAGCACCTAGTGGAATTTAAAAGTGAACAAGACCAACTGATTTTTGGAGGCGTAGTACAAAACTCCGATTACTTTTTTATGGTGACTTCACTCGGTAACATTATTACCGTTCCCATTTACAAAACGTTAAGTAACACCAAAACCAAGATGAACGAGTTGTTGGCCAAAAAACCGATCCTAATGGAAGATGAAAAATTGGTACTAGCAGGGATTGTAAACCCAGACAAAATGGAACAACAGCTCTTGGTGTTAACGTCACAGTGCGGTATGGTCAAACGGGTCGAGTTGAGCAAAGTCATTAACACGAAGCAAATTAAGTCAAGCTGCTGCATGGCACTCCGGGAACGCGACAAACTGGTCAACGCCTTTGTTCAAACTAAAAGGGAACCTAAACTAGTTTGTCTGGTGTCATCTTCCAACAGTTTTGCTACCTTCTTAGCGGAAGAAATTCCAATTATTAGTAACAAGGGAATTGGGGTGAAGGGGATTAAACTCAAAGCTGAGGAAAAGGTACGGTTTGCAATGCCGTTACAGGATAATGATGCCTTAGTAGTGATTAACAGTGATGGTGGTGTCTATAACTTTGAAGTAGTGGAACTGGCAGTCGCTAGCCGCATGAGTGTTGGTAAAAAACTAATTCCCAAAACCAAAACCCCGGTAAGCTGTTTTGCTGCTAATAAGCACAGTGAGATTATTGGTCATCGTGGTAAAAATGGTAGTGATCTGTTTACCCTAAATGAGTTAAACCGTTTACCAAAGTCAACCGTTAGTCAAATGCGTTTGTTTAAGTGGAGCTAGTGGTTTTACCACCGTGAAGTTGCTTATCGTACTCCTCGATCTCTTCTTTGAGGGTCAACAATAACGCTTTCACCTTTTGGTAGTCCATCCGGGTGGGACCCACTATCGCTAGTTGGTGCTTACTTTCGTTGGTGGTATTAATAAGAGTAGAAGCTACCGAAACCTCTTCCCCTTCTAATCCTAACTTGTCACCAAACGTAATATTAGTAGTCTGGTTGTTTTGGTTCATAAAGGCCATTTGTTGTCAGACACTAGTATCTTCCAGTAAGTTTAAGATACGCGTCAATCTTTCCTGGTTGGCAAACTCTGGTTGTTGGGCTAGGTAATGGATGCCATAAACCTGCTTACGTGCTTGTTGGAATTCCTCAAAGTTAAACAGCTTAAACAGGATTTCGTCAATGACATACTGGTACTCGTGTACCTTGCTGCGAATAATTTCCTTTAAAACATCTAAGTGTTGGGCAATATCACAGAAACGTGTATCGATAATGCGATCATTAAAGATCCTGACACATACCATGATGTCTTCAAACTGTCTTTGGTTAGCATAGCTAATCGTTTTCTTAAAGACGTTACCACTAGCTGACACTAACAGTAGCAGTGCACAGGACTGATCGAGCATAATGAGATCAATCTTTTTTAAGACTTCATCACTACTAAAGTGGGTTAACACCACTGGTAGGTTTACCATTTCGTTGATAAACTTTACCCCCAGTTCAATAATTTCATCAATTGTTTTGTGTTTTTGCAAGATAATCGCCCTTAAGCGGGTTTTAAGGTTACTCTTATCATCATTTTTAGTGAGTAACTTGACATAGTACTGATAGCCCAACTGGGAGGGGATTCGTCCTGAAGAGATGTGGTTCTTTTTGAGATAACCCTCTTTTTCCAAAACGGCCATTTCGTTTCGGAGTGTACCGCCAGAGAGGTTTTTAAAATATTTTTTAGTTAATAATTTACTACCAACAGGTACTGGATAGGCAATATATTCGTTGATGATGGCCTTGAGAATTTGGGCTTGTCGGGTAGTTAAATTTTTCATTAGGTTATCCTAATTTTATTTTGTTAGCCGCTTGATCATCGTGAACAATACGCTCGCTTTTAAGTTAAAGAACGCACCCCATAAGCCAGGCTGCTATTTGTGAAAGGATGACGCTGGTCAAGTTTTGTATGTCGGGAAGGCTAAGGACATCTTTAAGCGGGTACACCATTACTTTAATCCAAACCGTTCCTTTAAAACACGGGCACTAGTGGAGCGAATTGCGGATGTCGAATACGTCATTTTGAAAAATGAAAATGATGCTTTGAACTTAGAGGCCAAACTAATTAAACAGTACAAACCGCGCTTTAATTTAGTTTTAAAGGAAAATAATGGTTATCTCTATTTCTTTATTACCGCCAGTGTTAAACCCACCTTGGAATTGGGCCGCCGGTATGAGTTTTCCAAGAATAAGTACTTCGGTCCGTTTGCATCGAGTAAGTTTCGACTGCGTGACATTTATGACCTGTTGTTAAAGCTTTTTCCATTACGCAAGTGCGCGCCCCATGAACGGGGTCACCCCTGCTTTTACTATCAGCTTAAAATGTGCATGGGTCAGTGTATGGGCGAGGATACACCGGAACGTTACCAGACCACCGTCAAGGGAATTGAACAGTTTTTTAACCACGGTCCCGAGCAGGTTTTAAACCACTTGCAACAGCAAGAAATCAAGGCTAGTGAACAACAAAACTTTGAAGCAGCACGCCACTTCTTGGACTTACAGAAAGCCGTTTTAGAACTAGTGAACATGCAACAAACGGCCTTCATTAAAGCAAAGCAAAGCCATGACTTTATTGGTTATGTCTTTGAGAAAAATGTGTTAGCCATTACCGTCTTTGCTTACGTTGATAACCAACTAATAGGCAAAAACCAGCAGGTCTTTGAACTACCACAGGACGATGAAAAGGAAGTTGAAAGTGCTTTGGTTACCTTTATTTACCACTATTATTCCACCAATAAGATACCCAAAACCCTTACAGTTTCTTTAAGCGAAGAGAATTTGAGCTTACTGGCCAACAGTCTTAAAATTAATGTTACGCAACCAAAAAACGGCGAGCAAAAATCCATTTTGCAAACCGTGATTGACAACGCCCGTTATGCCTTAAACACCAAGTGAACTGGGTTTATCAATAACCTTAACCGTGCCGAAGTGCACCAGCAATTAGCGCAGCTTTTACAAGTTCCTTCGATTCAAAGTCTGGAAATTATTGACATCTCCTTTTATGACAAAGACCACGTGGTGGGGGCGATGTTACGGTACGAAAATGGCAAACGGATGAAAGCGTTATCACGCCGTTACAACATTAACATTGACCATAAGGGTGACACTAACTATATGGCTGATGTGGTGTACCGCCGGATTATTTCGTCCATCCAAACCCATAAACAGCTACCTTTAAGTGATCTTTTAATTGTCGATGGTGGGATTGCCCAAATTAATACGGTAACCAAGGTGTTTGCCTCTTTCCCTAACGTTACCCAACCGATCATTATTGGCTTAGCGAAAAACACCCGTCACCAAACTGACCACATTGTGTTAACGGATAACACTACCATTAACATTGACAAAAATACGCCTTTGTTTGCTTATCTTACCACCATTCAAGAAGAGGTCGATAGCTTTGCCAAACACAATGCCTTTAAGCGAGTTAGTCGCGCTCGCTTCCAAAATCCACTCTTACAAATTGAAGGGGTTGGGCGAAAAACTGTCCAAATATTACTTGATAATTTTCAAACAATGCAAAACATTGAAGCTGCTTCTTTGAGCGAATTATCACAGTTTATTCCTGCTAATTTAGCACAAAAAATTAAAACTTTTCTAAAGGAAAATGACTAAAGTATTAGTGTTATCAGACACCCATGGCTATAACGATAGGTGACTAGCCGTTATGAAGTTGCACAACCCTGATGTAGTAATTCATGCTGGTGATCATTTAACTACTAAAAAGTTTATGGATCAAAATGCCACTTTTTGGGTTGCTGGTAATCATGATGTTGTTGGCGAAGAGATTCAAATGTTTGAGTTAGAGGGAATCCAGTTTGTTTTAATGCACGGCCATCAAGCGCCCCGTCATGATCTCAAGCAGTGGTATAAAATGCTGGTTGATCAAGCTAAAAGCTATCTTTGTGATGTTTTAATTGTGGGTCACAGTCACATAGAGCACTATGAAACTATTGATGGGATCCAGGTTATTAACCCCGGTTCTCTAGAAATTCCCCGTAATCCGAGAAAGCTACCTACCTACTGTAATTTAAACTTAAGTCAGGGCAGGATAAGTGATTTAACGTTCCATTTTCCTCGGGATTAAGTCAAATAAATAAAAAAAGCCATCTACCTAACAAGCAGAAGGCTAAAAAATACTTTTTTAGCGATATTGAAATTAACAGAAAGATCTGTTAGCAAATGTAAGGAACAAGCTTATGCAAACTTTCAATATGTGACGTGTTGAAAAACCTTTAAGAGTATGCATCAATTGGGAGTAGGAAAGCAGGCTTTCAACACAAGTTTTCGTTTTCCTGCTCCCACTAAATAAATTGGGAAAGGTTTTCAAAGTCGCCAAAAAGATTAAATATTTTTGGAATTTTGAATTTTTAGTTGCTTCTAATTGTTAATAGAGATTTACTTTCAAAAAAACTTTTTGGTTTATTTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTTAGTGTAAAGACTCATAATGTTTAAATTAAAAATTAACAACTTCCAATTAGGTTTCAAACTCGTTCAGTGACCAGTGACGAACCACCTTCTTAAACATTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTGCCATTAAACGCATAATTTCACTTGCTTTGTTTAAGAAAAGACTTAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGGATTTAATGGCAATTTCAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAACAATCAGCGTCTCATAAAGATTGAAACTACTTTGGCTATCCAAGGCGAACAAATCAACAAATTGACTCAAACTGTTGAAAAGCAAGGCGAACAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCAGGGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAGCACAAAGACAAGAGTTTAATGCCCGCATGGATCGTTTGGAAAATCTTTTGGTGGAAAGTATAGAATCTACCAATAATCGCTTCGACTCTATGGAAAGACGTTTAGACTCTATGGATAGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTTGTCTTGATTTTGTTGAAGGACGCTTAGACTCTATGGAAACTCGTTTAGACTCTATGGAAACTCGCCTGGACAAAGTCGATCCGCCCAAATAGTTTTTAGTTAAAAAATAAGGGTATCAGCAGTTAATCTTAAAGATTAGTTGCTGGTATTTTTATTCATTTAATGCTTTCTAAAAACTTTTTGTCAATTTTGTTGGCACTTCTAAATTGCTAGTTCAATTTATTAAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGTAAAAAATCTGATCACCGTTGGTAGCATTGCTGCAAATAGTAGTTATGTAATTTGCAAACAGAAAGGAATTTTTCAATATGAGTAATACAAAAAAATTAAGAATAACTTCAATCGAAGAAACAATAGAAATAATAGTTAATTCAAAACTATTAGCTTTTGGTTTTTACCCAGTTGGTTTAGAAGATGGAACTTACGAACTTAATGTAAGGATTATTTTTAAAGAAAAAACTGCAGATTCTGAGAAGAGTAGCAGTGTACATCCTTTACAAAAGGAGTATTATGTAAGGGAATTTTTGAGATTTCTTGAGCGCCATAAAGATTCTCTGGCGCAACAACTATTAGTAGTAGAAGACAATTAAATGATTTCTCCCAAACTGGATTGACTCATCCTCACCGCCACCGCCTCCCTCGCGGCGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGAACGTATCGTAACACGAGCTTTTCCTCCCTCCCCCTCACGGGTGAAAACCCCGGGGCGTGGGCCTTAGTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGAACCTGCGCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGAGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATCCAGCAAGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGAAGGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCCTTTAATTGCTCTTGTTAATTTAGTTAAGTTTTTGTTTGCACAAAATTAAATTTTCTGTTTAATTAACTATCAATATTTTGCTGCTTTAAAATTTAAAGCAACTTTTAAACAGTAAGTTGTATCTTTTCACAAAGATTTAGTTCGATAAATTATTGGTAACAAGAGGGATGGAAACAACCGCAAAAAGTATTGATTGCAAAGAATTAGGCCTTTCTGTAACGACGAAGCAACAACTGCATAAATTAACACTTGCTACTATTTGACTTTGGGATTCTTTGTTTTTTGTTTTCTCTTACTTTTGTTTATAACGCTTACTTATACGATCTATTTTGAGCAACACATTTTGGGAAGTGATAAACCACTTCCTTTTTGGTGATACATTAAAAATCATGTGGTTCCCACCTTTGGCGTCTTAACTTTAGCTTGTTGGTGTGCGTTGATTGGGATTAACTTTTGGGTTTACAAACACACTTCACAAACAGAGCTGTTTCACAAATCAATTAGGTGACTTAAATGAGGTATTTTATTACCGCAAATTGCGCTAGCTAACTCCCTTTTCATTAGACAGAAACTAAGGCTAATGGAAGGCGAAGAACAGTTGAATAACAACAAAATAGAACAGAAACTAAAAGCTTTATTTATTAGTTTCTTTACTATTTGAGCAGTACAATTGCTTTTAGGTTTTTTCTTTTGGTTGCCGGTCTATGGTCTCTTTTTTTGATATATATACCAAAGCATCGATTGTAGAAGCTGTACGTTTTTCTTTTTTGAATACCTTTCAATTAAAGGAACTTGGTTTACCGATTAAAGAAATTGCTGTACCATTAGGCACATTAGTCTTTCTTTTTGTTGTTATTATTACCTTAATTCCGTTGTTAATTATTGGTAATTTAATTTGGACTAACTTGCGTTTAATAGAACGGGAAAATACACAGCAGTACCAACTCGTTTTTGGTTACAGTTTGATAGTTAGTGATATTGTTGGTTTTGCTATTGTTTTCTTCGGTGCCATTTTAGGTACAAATTTAAAAAGTGTTGAATTATTCATAGCATTGGGTTGAATGATGATGTTAGGATCATTAATTGCATTGGGCACTACTGTTAACTTAGTGAGCTCAATTTATCTCTACATAAAACTAGCATTGAAGAGATAGACACACTTCTCCCCCACGCTCACGCGCTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTTCTCGACTAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCCAACGTCCAAGCCACGCTTGGTGGCAGTCAGAAGACGACTACAGGCGGCTCCAAATGACCCACCCTCGACCAAGCCAACCTCCTGCTCTGAACCGGCGCGGGGTGAAGGAATGATAAGAACGGACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGCAGTGGCCAGCAGGGTGGTTCTTCAGGTACAAATACCTCAGCGGGGAATCCCGACTCGTTAAAGCAGGATAAAATCACCAAGGAAAGCTCGGGTAACCTCAAAACGGAGGACGGCGCTGCCAACTCTAACGAAAACAAAGACAACACTAATTATCAAAATTTACCCGCTTATGGGAAAGTTAGTGGGTGGATAACACCTGATTATTAATAGGCTTTTTACTTTCCAAAAGTTTTAAAGTTGTTTTGGCACTTTCAAACTTTAAAGACCATTTAAAAGGCATAAAAGGATAGTTATGTCTTTAAGAAATATTTTGCGATTCAGTTCGCTTTTGGTTTTATTTGGTTTTGAGTCTTTTCTAGTTAATGCTCAAATCTTTTTGCAAAGTTTGTTGTCACTTCTAAATAACCTACTCAATGTATTTAGTGGAGGAACAAAATGCAAATCTTTAATTTTTGAAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAAAAGAAAGGAACATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTTTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGCTGACACTGAGACGACAAATAAGGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTGATTTTTTGGTCTAAAAAGACATAAATTTACCAACAGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTCCCCCACGCTTTCCCGCTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAATGTCAACGGCACCTTAAGTGGTTCGCGAACTACGCCTAATCAGCAAGGCACTCCCTGGTTAACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGCGCGGGGTGAAGGAATGATAAGAACGGACAAAGTGACGAAAACTACACCAATTTCGCGAGTGCTACGGGCAGTACGAACCAGCAGGGTTCAACAACGGGTGGATCAGCGGGCAACCCCGACTCGTTAAAGCAGGATAAGGCTGATAAAAGTGGTGATTCGGTAACGGTTGCAGAAGCCACATCGGGCGACAACTTGACGAATTACACCAACCTCCCCCCAACCTCACCCCCACATCCGACTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCCCAGCGGGTGCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTCAATAAGAGTGGGCAAGATGATAACAGTAAGTTTAATTCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGATTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCTTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCAGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGCTCGGCGGGTGGTTAGTTACGGTTCACGGACTTTATCAAACCCCGCGCGGGTTATTTGGGCCTCCAGTTAAGTGGCCTGGATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCAAGCGGCCCTGAGCGGCATTTCGTGGCAGTTGGGTCAACCGGTTGGGACGGGTCGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGACACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTCCACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGCTCCGGTCAAACCAATTCCCAAAACACCTCCCCCTACCTGCATTTGATTAAACCTAAAAAAGTTACTGCTTCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGGGTGAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTGTTTGGGACGAATAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGTACCACGCAGGACTCAAGCACCACCAATCAACTGTCACCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGAGGTGGTGGGGGTGGGAGATTTGTCTAAGCGAGCTTCCATTGAATCAAGTCGTTTGTGAATAGCATTAAAACCTTAGCTAACGCTAACTTTGGGCGATTTAATCCACTTAGTTTTGAACTCCTTAAATTTGTGTTTTTCTTGACTTTGCTAAAAAAACAATTCAAAGAGGCTTGCAATGAACAGGTCAGCACTGCTAAATTGGTGAGTGCAAGAACTAAATAAAAGTAATTATTTTGTAATTTTTCGGCGGTCTTGCCATACATCAATTAAATGCTTGATCAAAACCAAGGTGTCAAAATTTAGTGCACAATTGCTAAGAGCGCCTGTATATTTAGAGTAAAACTACTTAAATTGCTAAAAATTAACCGTTTTTGAATTAGTTTTAGGGTCAAAATTAGGCCAATTTCCCGCTTTCTTTTTTTAAAAAATCTCTACTTTTTACCCCCTAAATAACCTAACAACTACACAATTAATTCCCAATTAATGTAAATTTATCTAAATTGTTCACCGTAGTTAGTTCAATTTAGGTAAAGATGAAAGGGTTTTCTTGCTCCAGACCGGGCTATCTTACTGGTCTCTTGCTCCTCGCTGTAGCCCCTATTCTCACTGCTTGTACCCGCGACTATACTACCAAGAACGAATTTCAATTAACAACAGCTCAACAGGCCAAATTAAAGCCAGCCACCATCGAATATTGGCGTGATGGTGATACGCCTGAGATCAATTACGCAAGCGAAGAGCGCCGAAAGGAAGCTGAACAAAAATCTAAAGAAAACGCTAAGAAAGAAGACAAAAAAGAGGAAAAGAAAACTGAAGATTCCCAAGATTCTTCTTCTGCATCCACGCAAGTTCGTAGTAGTAAACACGGTCTCCGCATCTATGGAATAGATACACCCGAAAAACATGTAAGTAGCAAAGGCGATAGCACCGGTGATGAAAAAATCGAAGCCGAAAAGGCCTCTAACTATGCTGAAAAGCTAATCCCTAAAGGCAGTACTGTTTGGGTTTGATCACTCAACACTTACAGCTATGATCGTGAAGTGGGCGCTTTATTCTTTAAGTCTAACCCTAAGCAAACCTTCTTCCAATCCTTTGAAGTAGCTATGGTTGAGGCTGGTCATGCTATTCCAATTGCTGGGACTGGTTTAAATTTAATCGCTGATCCGGAATTAAGTGCTGATGATCCTTTAAGTGTCATTGGTCTACAGTTAGCCAACGCTGCCAATAAGGCTTACAACGCTAAAATTAACATTTGGAGCCATGACACTGATGGTTATCGTTCCTTGACTGCTGTTTACAAGTTAAGGGGCGCTGATATTAGTTGAACCCGTTTCCTAGATGAAGCCAATGGATATTCCTCTGCTAGTGCTGGAACCGGTGCTAGTTTGTACCAACTCTGAGATCAACGTCAAGCCAAATTAGCCCAAAAGGGTAGTTAATGAAAGCAGTTTAAATCAGCAATATGGAAAAGATACAAGCGGAAAAATCCCAAAGTGCAATTGAGTTTAAGAATATTGTAGTCGACTTTGGTGAGTCAATCGCAATTGATAACATTAACCTCACGGTTAAAAAGAAGGAGTTAGTTACCTTACTAGGTCCTTCTGGGTGTGGGAAAACAACTTCGCTTTCCGTGATTGCGGGTCTTATCGCCCCTACTAGTGGTCAGGTTTTATTTAATGGTTATGATGTCACAAAAAAGCCACCACAACAACGCAAACTGGGATTAGTGTTCCAAAACTATGCCTTGTATCCGCACATGAGTGTGTTTGAAAACATTGTCTTCCCACTCTACAGTGATACGTCTTGACGCGAAGCCATCTTTGAAAAAAATACCTGAGCGCAACACGACATTAATTGCTTGATCTTAAAGGCTAATGGTGCTACTAGTGAGGAGCTGGCAGAGCTCAATCGTTTAATGCAACAACGAATCGATGAGCCCAAACGAATGGCTTACCAGATTAATGATTTGATGGTAAGTGTGTTTCAAAAACAGAGTGAACTGGAAGCTAACCTAAAGTTAATTCCCCGCAAAAAACAGTTTGCCATTATTTCACTCAGCAAGGAAACACTAAGTCAAATAAGGGATGTTGAAACTAAAGCCAAGGCTGCATTGGAAACCGCTGATAGTGCTGAAGTGGAGCAGACCATTAAGAGTGAACTTAAGCAAAAACTAAGTGAAATTAAGGCTAATTACCACGATGAAAAGGCCAACATTAAAGCTTACTGATGGGAAATGTTGGCCAATATTAAGACCGAATTAAAAACCGAAAAAACTGCCATTAAGCAAACTAATGACTATGCCAAATTGAAGGAATTGAAGTGAAAGATCCACTTTGAACCCTTGAATTTAAAGAAGCAGTACCGCAGTTACTTTAAACAACTAAAAGCCAAATACAGCTTAAAAGATGGCAATTTAACTGAAAGCGAATTAAGTCAAATTGAGGAGTTGCAAAAACGGATTGTTTCGCTCAAAGATTTCATTAACCGCACTGCTAAAGAAGTAGCTGAAAAACTGGAAATTACTAAGATTCTCCACAAGCGCCCCGCTAATATTTCTGGGGGTCAACAACAACGGGTAGCTATTGCTAGAGCAATTGTTAGAAGACCTAAGGTTCTACTTATGGACGAACCGTTGTCCAACTTAGATGCGAAGTTGCGGGTACAAACACGGCAATGGATCCGTAAGTTCCAGCAAGACCTGCAAATTACTACTGTCTTTGTGACTCACGATCAGGAAGAGGCAATGAGTATTAGCGACACGATTGTCTGCATGTCAACTGGTAAGGTACAGCAAATAGGCTCCCCTTCCGAGTTGTACTTAAAACCGGCCAATGAGTTCGTTGCTACCTTTTTGGGTAGCCCGGAAATGAATATCGTCAACGCTACGGTTAAAGCTGGACAGCTGTTGTGAAACGAAAGTCCCCTAGTTAAAACAAAGTTTGATTTACCTGATGGTGCCATTCGGGTTGGTTTCCGTTATGATGAGGTAACGGCTCCCAAAAACGATGGTAGTCCCGTATTTAGCGGTACCCTTATAAGTGTGGAAAACCTAGGGAAACACATGGTTGGGGTTGTGGAGAGCAATGGGGTACAGCTCAATGTGCGCTTAGAATTGAGCCACCAGTTTGAAGTAGGTAATGCAGTTAAGTTTACGATTAAGCCTAATGGCTTACACTTCTTTGATCCCCAAACAACGCAACGGGTGGAGGTAAAACATGTTTAAGTGATTGTTAAAGCACCGCTCTAAACCAACGCCATTGGAGCTGGGAATCTTTGACCAAAAGGTTTCCTTTTGAAAACCATTTTTGCTGTTTTGCCCAGCTTTATTAACTACCTTTCTCTTTACTTTAGTACCGTTCTTCCTTACGTTACAGAAAGGGTTTAGTCACAATGAGGACATCTACCGGGTTGATTCCCAACAGTTTGGCTTTCAAACTTTCGCTAACCTTTTTAGTGAGTCCAACTTTATTTTGGGCTTGCGCAATTCCTTTTTGTACTCGATTATTTCCCTACCACTGACAATTGTGCTAGCAATTATTATTTCTAGTGCCATTGTCTTTGTGTACCGGAAGTTAGCACGGGGCTTTTGACAAACAGTATTCTTTTTACCCTATGTAACTTCCGGAGTAGCGGTTTCGATTGCTTTTATTTATATTTTGGACTCCTCTTCGGGAATCTTAAACAACATCTTTCATGTCAACATTAAGTGGTTAGATTCGGGGGAACGTGATACCTTTAATGCCTTATGGGGAATTCTTATCTTTGGAATTTGGAAGAACATGGCTTTTAATGTGCTGGTAATATCAACCGCCATGTTAAGTGTGGATCCTACTTTATATAAGGTAGCTAACTTAGATGGTGCAAAACCAATTAGGCAGTTCTTTAAGATTACCTTACCTTCAATCCGCCCAACCTTAATTTTCCTGTTGACCCTGTTAATTTTGGGCGGAATGCAGGTGTTTCCAATTTCGTTGTTTAACGGGAATGATAGTGAAGCTGTAACCAATGGGGGTTCGACCATCTTGCTTTACATCTTCCAAAAGATTCGTGATCAGAATAACAATTTTGCTGGTGCTGCTACCTTGGTGTTGTTCATCTTGGGGGTTTGTTATGGGTTAGTGTTGCGCAATGGTTTTCGCTTAATTGAATGAGCGCAGTGAAAGATAAAGAGACATTATGTTCAAACAAAGCTTAATCTGGTATAACCGTTACGTCCAAAAACGGAAGAAGGGTTTAGATCTCACCATTAAAGATCGATCTTGATCGAATGTATTGTTGGGTTTAATCTTTAAAACGGTGGTGTTGTGCTTCTTTGGTTTAATGGTCATCTTTCCCTTTTATTTGATGCTGGTGGTAGCACTCACCAGTGATGAAGTGGTCTTAAACATTCGTGAACCAATTCTCAAATCCGATGGGTGGCACTTCGAGAACTTCCGCCGTGTTTTGGAAGATGGTAAATACCTTAATGCCATTTGGATTAACAGCTTAGTAACAATTTTGTCGATTATCCTACGGCTTTTCTTTACCGTTAGCATGGGCTATGCCTTTTCGTTAAAAAAGTGAAAGCTCAAGAAGCTGTTTTGGTTTATCTTTTTGGCAGTGTTAATTTTACCCGAATCGGCCTTGCTAATTGGTCAGTATCGTGTGGTGGTAGTTGCCAATTGAAATCAACCAGAAAAACCAGCGATTATCTTGGGCTTAACAATGCCATTTGTCGCTTCTGTGTTCTCTGGGTTTATGTTCCGTACTGCCTTTGAAGCAATTCCACCACGAATTAAAGAATCGGCCTTTGTTGATGGTTGTACAGGGTTGAGGTACTTCCTCAAAATTGCCTTTCCAATGGTACGTAGTACCACTTGAACGGTAAGTATTTTAACGGCCTTTGCGGCGTGAAATTCTTACTTATGACCGTTATTGTTACTAACAAACAGACCCGATCTCAACATTAACCTGTGGGTGTTGGCGCAAGGGACTGATGGCAATGCCGGGCAAAGTGACGAACAAATCCGGGTGTTGTTGAATTTAAAGATGGCAGCAGCCATTTTAGCAATTCTGCCAATGTTTATTGTTTACTTCTTGTTTAGGAAAAGGATTATGAAAGCCGTGGGATCACGTGCCAATACGATTAAAGGATAGTTATGGACAGCTTAAAGCATTTTTGACAACAACTAGTAATGTGATCAAAAACTGTTACCTTTTGAATCATAGTAGCAGTAGGGATTGCTTTTTGTGCCTTTGCCATTTGGGGCATTATTGCTTGCATTGAAACGATTATCAAAGAAGGGGCGTTGTTGTAAGTTAGCTTTCTTAGCTTTTTTATAAAAACACCAGATTGATATTTAGCCTTTTTGAGTTAGCTGTTCTCACTGCTTTTGGCGGTTGCTATACAGAATGAAGGTCGTTTGTAAAGGAACGTAAACTTTGCTAACTTAAAGATCTCTTTTTGGGTTTTAGGTGTTTTATTTTTGTTACATAAAATGAAGAAAAGGTTAGGAAACTACTTGCAATCAAAAAAAGCGTTTTAGGTAAAAAAAGTGCTTTGTTTCATAACCACCCTTCGAGGTGGTTTTTTATTTGTTTTATAGCTTTGTATACGAATTCACTCTATAGATATAGGGTGCTTTTATTTTCTGGCAAGCTGAAAAGGGTAAAAAATACCAAAAGTTAGCAGTTAAGAAACCACTAAGAATTGGTATTTTGGTTTTACTAAATACTATTTGGGTGGATCGATTTTGTCTAAGCGAGTTTCCATTGAATCCAAGCGATTTTCCATAGAGTCTAAACGTCCTTCAACAGAATCAAGGCAACCTTCTATTGAATCCAAGCGATTTTCCATAGAATCAAGACGACTATCCATAGAGTCTAGACGACCTTCAATAGAGTCGAAGCGTTTATTGGTGGATTCTATACTTTCAACCAAAAGGTTTTCCAAACGATCCATGCGGGCGTTAAACTCTTGTCTTTGCGCTTTTTGCTCCACTTGAAGTTCTCTAATTTGCTCGCCCTGGATTCTTATTTGTTCACCTTGGGCAGCCAAAGTGGTTTCAATCTTTATGAGTCGTTGGTCGTTTGAGTCTTTGTACTCGTTAAACTCTTTGCGCGTTACGTATTCCGTGTTCACTGGTATAACCGGTTTGTTAAGTTTAAATTGTCTATTGAAATTGCCAGAGAATTCCACACTTTTGTTTTCTGGATTAATGATGTATCAGCCCGAAAAAGTGCCCTTTTTGGTCTTTTTCATCATCTCGTTAAATTCCTTTTCGTTATAAAACGGAATTTTCTCTTTCATTAATAAGCGACTCCTTTCTTTTCAGTGAAATTTAAAAAGTAACTACTACTTGCAACTCAGTTGCCAACAGGGATCAGACCATTTACTGATCAATTTAAAAAATAAAAGATTTGCATTTTGCTCCTCCACTAAATAAATTGACCGGCTTATTGAGAAGTGCCAACAAAATTGCAAAAAATTTCAAATGAATAAAAATACCAATAGCAATTTAGCCATTGGTATTTTTTAGTCTACAGTTATTTAGGCGGATCGATTTTGTCTAAACGAGTTTCTATTACGTCGAGGCGTTTACCAAAATCAGTCAAAGTGTTTCCGATTGATTCTAGACTTTTAAGCACAAGACTTTCAAAGCGATCCATGCGGGCGTTCATTGCTTCACCCTGGGTTTTGATTTGTTGACTCTGAACCATAACAACTTCAGTTAGTCGATCAACTTTGTCCTCAACAACTTGAATCCTTTGACCCTGACTCTTTAACTCAGTTTCAATCTTTGTAAGTTTTTGGTCGTTTGAGTCTTTGTACTCGTTAAATTCTTTACGCGTTACGTATTCTGTGTTCACTGGTATAACTGGTTTGTTAAGTTTAAATTGTCTGTTGAAACTGCCAGAGAATTCCACACTGTTGTTATTTTTATTGATGATGTATCAACCCGAAAAAGTGCCCTTTTTGGTCTTTTTCACCATGTCATGGAATTCTTTTTCGTTATAAAACGGAATTTTCTCTTTCATTAAGCAATAACTCCTTTCTGATTAAAGCGGTTCAAAAAACTACTACTTACAACTCTTTGTCATTGGACTTTTCAAAATTTGATCATTGTTTTAATGCTCCTTACTAACTAAATTGACCCGCTTCATAAAAACTGCCAACCAAGTTCAAAAAAGTTTTTAAGCTATTTTTGCACCAAAATGGCGTTGTCAGTCAAACTACGACAACAACAGTTGCTGTTTGATTCTTTAAACTTAAACAGAATTAGCACCAATGAATAGCCAAGTACACCGCAAGGGCTCTATTGCAGAAGCAGTTAGTGCCATCCAAGCTCATGATAAGATCGTGATCTTCCACCACATTCGTCCTGATGGCGATTGTTTGGGCGCACAACACGGCTTAGCGCGTTTAATCCAAACTAACTTTCCCCACAAGCAGGTCTTCTGTGTTGGTGATCCCAAACACAACTTTCCCTGATTGGAGATGGTTTTCACTCCAAAGGAACAGATTACCCCGGAGTTAATGCAACAAGCCTTAGCCGTTATTGTTGATGCCAACTATAAGGAACGGATTGAGTGCCGGGACTTATTAGACCAAAACCAGTTTAAGGCAGTATTGCGGATTGACCACCACCCCAATGAGGACGATCTCAATACGACCCATAACTTCGTTGATGCGTCTTACATTGCCGCGGCTGAACAAGTGGTAGATCTAGCGGTGCAGGCCAAATGGAAGCTTAGCCCCCCAGCGGCTACGGCGCTGTATTTAGGTATTTATACAGATAGTAATAGGTTTCTATATAGTAATACATCATGAAGAACACTATATCTAGGATCTATGCTATATAGAGCTCAAGCTAATATAGCTAAGATCCATGATGAGTTAAACCACACTTCCTTAAAGGACATCCAGTTTAAACAATATGTCTTTAAAAACTTTCAGACCTTTCAGAATGTTATTTACTTTGTGGCCGATAAGAAGTTCCAAAAGAAATTAAAGGTAACACCCTTAGAATGTGCACGGGTAAATATCCTAGCTAACATTGAACAATTCCACATTTGGCTGTTCTTTATAGAAGAGGGTAAGAACCACTATCGGGTCGAATTCCGTAGTAACGGAATTAACGTACGCGAAGTAGCTTTAAAGTATGGTGGCGGGGGTCATATTCAGGCCAGCGGTGCAGTTCTTAAAAGCAAGCGCGACATAATTCGTGTAGTTCAAGATTGCCAAAAGCAAATTGCTGTATAATTTTTAACAACTATGCACCAAACCAAAAAAACTGCCTTGTCCAAGTCCACTTGGATTCTCATCCTCACCGCCACCGCCTCCCTCGCGACGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGAACGTATCGTAACACGAGCTTTTCCTCCCTCCCCCTCACGGGTGAAAATCCCGGGGCGTGGGCCTTAGTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCGCAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCGCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAGCAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGAACGCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAACCGTACCGCCATTGACCGCGTTGATCACTTGGATCCCAAGGCCATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCTGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGTGGCAATGGCCATGTTACCAAGTCGGCCCACACCGCGCCTTTGAGTATAGGTGTCTTTAGGGTGCGCTATAATGCAACTGGTACCAGTGCTACTGTAACTGGTTGACCATATGCCTTACTGTTCTCAGGCATGGTCAACAAACAAACTGACGGGTTAAAGAATCTACCCTTTAACAATAACCGCTGGTTTGAATATGTACCACGGATGGCAGTTGCTGGCGCTAAGTTCGTTGGTAGGGAACTCGTTTTAGCGGGTACCATTACCATGGGTGATACCGCTACCGTACCTCGCTTACTGTACGATGAACTTGAAAGCAACCTGAACTTAGTAGCGCAAGGCCAAGGTCTTTTACGCGAAGACTTGCAACTCTTCACACCCTACGGATGAGCCAATCGTCCGGATTTACCAATCGGGGCTTGAAGTAGTAGTAGTAGTAGTCACAACGCACCCTACTACTTCCACAATAACCCCGATTGACAAGACCGTCCAATCCAAAGTGTGGTTGATGCCTTTATTAAGCCCTGAGAGGACAAGAACGGTAAGGATGATGCCAAATACATCTACCCTTACCGTTACAGTGGCATGTGAGCTTGACAGGTATACAACTGGTCCAATAAGCTCACTGACCAACCATTAAGTGCTGACTTTGTCAATGAGAATGCTTACCAACCAAACTCCTTGTTTGCTGCTATTCTCAATCCGGAATTGTTAGCAGCTCTTCCCGACAAGGTTAAATACGGTAAGGAAAACGAGTTTGCTGCTAACGAGTACGAGCGCTTTAACCAGAAGTTAACGGTAGCTCCTACCCAAGGAACAAACTGATCCCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTTGTGGGGTCGGTGCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGCAATAACCACCGGGGCGTGGATGATATAACCGCGCCTCAAACCAGCGCGGGGTCGTCCAGCGGAATTAGTACGAACACAAGTGGTTCGCGTTCCTCTCTCCCGACGTTTTCCAACATCGGCGTCGGCCTCAAAGCGAATGTCCAAGCCACCCTCGGGGGCAGTCAGACGATGATTACAGGCGGTTCGCCTCGAAGAACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTTCAAGTGGACAAAGTGACGACCACACCAAGTTCACGAGCGCTACGGGGATGGGCCAGCAGGAACAATCAGGTACCTCCGCGGGGAATCCCGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGATGGATCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCGCAAGACGTTGGTAACCTCGTTGTCAGTGGCACCAGCTTCAGCTTCCAGCTCGGCGGGTGGTTAGTTACGTTCACGGACTTTATCAAACCCCGCGCTGGTTACCTCGGGCTCCAGTTAACGGGCTTGGATGCAAGTGATGCGACGCAGCGCGCTCTCATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGTTGGGCCGCGTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAAGGGACGCCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTATCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCAGTAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAAAAAGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGATGTGGTGGGGGTTGGTCGACTTTCTGAAAGCAACGCCGCAAAGATGAACGACGATGTTGATGGTATTGTACGCACCCCACTCGCTGAACTGTTAGATGGGGAAGGACAAACAGCTGACACTGGTCCACAAAGCGTGAAGTTCAAGTCTCCTGACCAAATTGACTTCAACCGCTTGTTTACCCACCCAGTCACCGATCTGTTTGATCCGGTAACTATGTTGGTGTATGACCAGTACATACCGCTGTTTATTGATATCCCAGCAAGTGTGAACCCTAAAATGGTTCGTTTAAAGGTCTTGAGCTTTGACACCAACGAACAGAGCTTAGGTCTCCGCTTAGAGTTCTTTAAACCTGATCAAGATACCCAACCAAACAACAACGTTCAGGTCAATCCGAATAACGGTGACTTCTTACCACTGTTAACGGCCTCCAGTCAAGGTCCCCAAACCTTGTTTAGTCCGTTTAACCAGTGACCTGATTACGTGTTGCCGTTAGCGATCACTGTACCTATTGTTGTGATTGTGCTCAGTGTTACCTTAGGACTTGCCATTGGAATCCCAATGCACAAGAACAAACAGGCCTTGAAGGCTGGGTTTGCGCTATCAAACCAAAAGGTTGATGTGTTGACCAAAGCGGTTGGTAGTGTCTTTAAGGAAATCATTAACCGCACAGGTATCAGTCAAGCGCCAAAACGCTTGAAACAAACCAGTGCGGCTAAACCAGGAGCACCCCGCCCACCAGTACCACCAAAGCCAGGGGCTCCTAAGCCACCAGTGCAACCACCTAAAAAACCCGCTTAGTATTTATGAAATCGAAGCTAAAGTTAAAACGTTATTTACTGTTTTTACCACTTTTACCGCTAGGGACGTTGTCACTAGCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGGGATACCAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGATCAAAGTAATACCAACCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTATGTCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGATCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGACCGGTGTATGAACCCCTGGATTCGACGAAGAATGGCAAGGGGAAGGATAAAGATGGGTGAACCACTTCCGGGGCGAGTGAGGCCAAAGGAGATGCCCCGTTGGTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGGTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCTACAAATGAAGATCTGCCGAATGTAATAACACAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCCCACACCGAGTTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGAGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCACGGGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGAACCAACACCGCCTCGCACTACCTCCCCGTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGAAGGCGAGCGGAGCAGCGGAATGGGGTGAAGATTAGCACCTTGGAATCGCAAGCCACTGATGGCTTTGCCAACTCGTTACTTAACTTTGGTACCGGTCTTAAAGCCGGTGTTGACCCAGCTCCAGTAGCACGGGGTCATAAACCGAACTATAGTGCAGTACTACTAGTGCGTGGTGGCGTTGTAAGGTTAAACTTTAACCCCGATACTGATAAACTGTTGGATTCTACTGACAAAAACAGTGAACCTATCTCCTTCTCCTATACCCCATTTGGGTCTGCTGAAAGTGCCGTAGACCTCACCACGTTGAAGGATGTGACCTATATTGCTGAAAGTGGTCTGTGGTTCTATACCTTTGACAATGGTGAAAAACCAACGTACGATGGTAAACAACAACAGGTCAAAAACCGCAAGGGTTATGCTGTGATTACCGTATCACGTACCGGAATTGAATTTAACGAGGACGCTAATACCACAACCTTAAGCCAAGCCCCAGCTGCTTTGGCTGTCCAAAACGGGATTGCTTCCAGTCAGGACGACCTCACAGGCATCCTACCGTTATCCGATGAGTTCTCCGCTGTGATTACCAAGGATCAAACATGGACCGGTAAGGTTGATATCTATAAGAACACCAACGGGTTGTTTGAAAAGGATGATCAGCTATCGGAAAACGTGAAGAGGCGTGACAACGGTTTGGTCCCTATTTACAACGAAGGTATCGTCGATATTTGGGGCAGAGTGGATTTTGCTGCCAACAGTGTTTTGCAAGCGCGTAACCTCACTGATAAAACGGTTGATGAGGTGATCAATAACCCCGATATCCTCCAAAGCTTCTTTAAGTTTACCCCAGCCTTTGATAACCAAAGAGCAATGCTAGTGGGGGAAAAGACATCGGATACTACCTTAACGGTTAAACCGAAGATTGAGTACTTGGATGGTAACTTCTATGGTGAGGATTCCAAGATTGCTGGAATTCCGCTCAACATTGATTTCCCTTCCCGGATTTTTGCTGGCTTTGCTGCTTTACCGTCCTGGGTCATTCCGGTATCAGTCGGTTCATCGGTGGGCATTCTCTTAATCCTGCTCATCTTAGGCCTTGGTATTGGAATTCCAATGTATAAGGTCCGCAAGCTTCAAGACTCCAGCTTTGTTGATGTGTTTAAAAAGGTGGATACGTTGACAACCGCTGTGGGTAGCGTGTACAAGAAGATTATCACCCAAACGAGTGTGATCAAAAAAGCTCCTAGTGCGTTGAAAGCTGCTAATAACGCTGCTCCTAAAGCACCAGTTAAACCAGCTGCTCCAACAGCTCCAAGACCACCAGTCCAACCACCTAAAAAGGCTTAACGATTAAGCCCCAACAGAAAGAAGCACAACTGTAAAAAGGTTGTGTTTTTTTTCATATTTACAACTTTTTGCTTTTTTGTTGGCACATTACCTTCCTAGTGCAATTTAGAAGGTGTATGAGTCATAAAGATTTTAATGGGCTTCAAGCCCCTCAGTTGTTAAGTTCGAGTTCCCCTGTTGCAAAGAAACAAAGTAGTCATAAACTCAGGCATGCCTTAAAGCACGCCCGTTACTTAAACCATAGTAGTAAGCGTACCCTCAAGCATGCCTTAGAGCTGCATGAAGACAACCAGGTCTTACTGGAAAAAGAAGGTAGTCCTAACTTCCAGGACTGGCTTTCTAAGCAACCTGGCGTCAATAAAACCAGCCTTAAGTACAACAAAAGCTTGGGTAGTTGAATCTCTAAGGAAAGTAAACCGAAAAAGCGTTTTCAACCGTACTTTACGTACAAGGACAGTAAAACTACTCCAGAAGAGGCCAAAGCCTTGCAGCAAATGAAACAATCCCAAAAGCGCTTCTTTCACGAAAATATGCACAGCTTTCTCAATGAAGTTGCACATAACCCAATGATCCAGCGCTTTAATCAAAAGCAGGCCAAACGGGCTGCTAATACGCGACAACGCACTTACAAATACCGTCAGTAACAATGCACAAGATTAAACACTATAAACCCTTAAAGCGCAAGACCTGGTTAAACAAGGTTTCCAAGCAACGCCGCGAGCGCATGTCCGCTAAGTGCTATCAACCACCTAAGAGAGAAGTGATGCAGCCAACTTGAAAGAGTTAGCACCACCTGATCCCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTTGTGGGGTCTGTTCTGGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGCGTGGATGATATAACCGCGCCTCAAACCAGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTTTCCCGACGTTTTCCAACATCGGCGTTGGCCTCAAAGCGAATGTCCAAGCCACCCTCGGGGGCAGTCAGACGACGACTGTGGGCGGTTCGCCTTGAAGAACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTTCAAGTGGACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGGATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCAGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGACCGGTCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCATCGCGCCCAGCTCTTCCTTCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGCTCGGCGGGTGGCTGGTCAGCTTCACGGACTTTATCAAACCCCGCGCGGGTTATTTGGGCCTCCAGTTAAGTGGCCTGGATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCAAGCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGCTGGGCCGCGTGGAGAGTGTGTGGGATTTAAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTGAAGCTTCCGGGTCAGCTTTGGCACCGCACCCGAATGCTTTGGCGTTTCAGATGAGTGTGGTGGAAGCGAGTGCTTACAGCTCTTCAACCTCAAGTTCGGGTTCCGGGTCAAGTTCAAACACCTCCCCCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCTAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGATCAATCTGGTGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAACTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACCAACTTTAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGGCTTCACCTCTTTTTAAAACAAGTTCAATTGCAACTTGTTCCTTTGTTTAGCCAAAACTTTTTGCAATTTTGTTGGCAGCTTTAAATAAACCGATCAATTTATTTAGCAATGACACAAAAATTAATATGTTGAATTTTTTAAATGAATCAACAATGGTATTGATCCCCGTTGGCAGCAGTGCTGCAAGTATTAGTTACTTTCCAAACCTTACAAAGAAAGGAGGTGATTTTTAATGTCTTACTCACCTTCACTTAAGGAAATTATTGCTATTTTGCAAAAATACACTTCGAAAAACTACCAGTCAAATTGCAAAACTAGACCCGATGGTAAGCTTGAATTATCATTAAATGGAGTTTTTGAAGAAATCGTGAAAACACCCGGTAAAACTAGGTACGTAACCCATAAACAGCTTGATCAGAAATTAAAAGATTTCAAGCAAGATCTGATGGTTGAGTTGCACGACACGTTTGCTACTAAAACTGATCTTAAAGAATCGGAAGCACGCATTAACCAAAAGTTAGAAGCACTAATTCAAATTGTTATGGTTCAGGGTGAGCAGATAAAAGTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGAAAATTGAAGCTCAAGGACAACAAATTCAAAAAGTTAATGAAACTTTGAATTTTGTAGTTGAAAGCCTTGGATCTATCCACAAAAGACTCGATTCAATGGAAGGCCGCTTGGACTCAATGGAAAACCGTCTTGACAAACTAGAGTCCAAGTAGTTCTAATGGAAATAAAAATTCGAGCAGTTATTCTTAGAGATTAGTTGCTGGTATTTTTATTCACTTTGAAGAGAAAAATCATTTGTTTAAACTCAAAAAGGTCAATAATTTTTAAAAAATGCTGAGGTACAACCACCTTTTAAAAATTCCTTAGATATAAATCGCTAAACTATCTTTATTTATTTTGTATGTGCCAAATTATTTTTGACACATCAAATCAAATATTATTTTTTATCATTCCCCTAAATTTCAGGGGTATTTGAGTATGCGATTTAAATATGGTGCAGTTGCTTTTAGTGGCTTTTTAGGTCTAGCAGTGATGTTGGCTAGTTGTGGTGCTAAAGGGCGTTTTGACCAGGTTGATGATGGCAAAATTAAGCTAGCTTCTTCACTTACCGGTAAGCGCGATGTGATACTGAAAGAAGTGCTCAACAAATATAACAGTCGCAAAGCCAAGGACGATTATCCGATTGAAATAACCAAAATAGCCGGTAGCTATGATGGTGGTCGATCTGATCTGCAAACGCGGTTGAGTGTCAAGGATAAAACAACCTTTTACAACATGATCTTGAATTATTCTGACACCATTTCTACTTTAGGTCGCAGTAACATGGAACTACCATTAGATAGCGTTGACGTATCACAGTTTTCAGAAAACTTCTTGAGCTTTAATGACCGCATAAGTGGTATTTCCAGAAAAGGCATTTACGGCATTCCGGTATCAATGTCAACTGATATCCTAGTAATCAACGGGCCGGTATTGCACTATATTTTGAATAGTGCAAAAAAGAAAGATGGTGCAGTAACTAAGAAAAACGCAAGTAATTCCAATGGTAACGAAGGAACGTTAACAGTAAATAACGATCAACAAACCACTGAACTATGAAAAAAGATTGAAGAAGCCGCTAAAACTAACGGTAAAACTACCCAAGAACAAACAAAAAGAGATGCAAAACAAAGTACATCCTTAATCCAACTAAAAGAAGGATCTGCAAATACAACCGAAGGCAATGCTTCAGAAAGTGATAAAGAAATCAAAAAATCTTGAGGCAATTACCAAGAAGTAGATGGTGGTCTTAAGGGCTATACCTTCAAAGCAAGCGTCTTTGAAAACTAAAGTGAAATGGTTGATTTTGCCACTAGGGTAGCGAAATCATTTAAGGATAAAGTTAGTGGTATCGATAATAAAAAAGGCACTGACATCCAAGGTGTTTTAGGTCTAGACAGTACACCAAACGTTTTGTTCGCTTCAGTATTTGCAGCTGGTGGTGGTAGCTATGACAACTTCTTTTACAAGGTAGAAAATGGCCGGGCTGATTTCCGTAATTTTGCAAATAAAGGTACTTCTTACAAAAACTTAGAGAAGGTTTACAACGATTACAAGAATTTAATCGGTAGCAACGGTCTTTTTGCTAGCAAAGATGGCTCTTACTCATCTAACTTTGAAAAGTTCCACCAGTTAGCTTTTTATGTAGGTTCTAGCTCTGGTTATAACTATGCTTTTGCTGATGAAACTGCTAAACGGTTAAAGTTTGGTGATAGTGCCATTGAGTATCCTAACGATACGCTTGAAATTAAGGTACCGACAAATAATAGTCAAAGTGGTGATGGGAATGGTGGCACCAATAACGACAATCTCTTAGGTACTTTTGACATTAGGGAAAAGAGTAACGGCAAAAAAGGTGAGAGCAACGGTAAACAAGGAAATGGTCAAGATAAAAAGACAATTTCGCTTTATAAAGATTCGATTCCTAAAGAAAAAACTGAAGGCACTGATGCAATTCTAATTAGCGATAATCAACTCATTAATCAATTACAAACTGCCGCTAAAACTAGTAGTTCACAAAATAAAGCTAACACTGCAGCTATAACTTTTAAGCAAAGCAATAAAAGTGAAACTGACCAAAGTACAACTAGTCAGTTTATCGGCTACACAACAACTGCTTCATTGAAAGCTGATAAGAAAAATATCTTTGATGTGAAGAAGCTAAATAACGAAAAGTCTGAGCGCAAAATCATTGTCGGTGCCACGGTGGAAACCTTAAATCAAGCAAACACATTGCAAGCTAATGAAGCCATTATTAAACCAGCACCTGGTAAGTACCAAAGTACTGATTCACACAAGGTCATGATTACTCAAGGCCCGAATATTGTCGGAATTCATGCCAACGAAAAAGAGGACAAAGAAACACAAAAGTTTATTAACTGATACCTCAATAAAGAAGAGAGTTGATCTGTGCAAAACAGCGGTTCAACTACAACTAAAAAGCAAACTGCTGCTCAATATTTTGCCGAACAGTCCTCTTACATCACTCCTTTAAAAAATAACTTTAAAGCAGATCAAAGTGCAACAAAAGATAGCACTGTTAACACTAACTATTTCACTAAACAAACATTTGATCTCTTCAAAGAAGTTAATGATGGAAAAGTGCTTGGTTTTAATGATCCAAGTGACTTTAGGAGTGGTAAGTTCCGCAATACTATTGGTTCTACTTTTAACGCAACCATCAGTTCTAAAGTTGACTTTAACAAGTTTTTTGAAAACTTTAAAGCTTCTTTAGGGTCTGGTTTTGAGCGTTAATTTTAAAGAAAATTTTTCTTTTCAATTGCGCTTAATCTAAAGGGCAAAAGTAACAGCAGTTCACTGCTAAGGTTGATTGTGTTAACCCGAAATTTCAATTAATTTCCTTTTCCTCAAAAGGTTTTCCAAAAAAAAGTAAAATAGAAAAGCTATTTTCACTTTTGATGCACAAAAAGAAGTTTTTATCAAGAACTTCCTTTGCTTCTTTGTTTTTGCTTTTAGGAACAGTATTGAGTGCTTGTAGCAAAGTAGAGTCCACAAAAAGTGTTCATCCTGTAAAAAGTTTTAAATCTGATCTAAAAAGTCTTCTTAAAGAAACAATTAAACAAGATATTGATTTATCTAAAACAATCAAAACTTCAGAAGGCAAACAACAGATAATAACTTCTTTAAAAAAGTCTTACGGAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCATGAAAACAGAGCTTTGAAGAAGGTGAAATAGGTATTGCGGATCTTGATTTTGAAGTAGCTTATCCCAAAACCCAAGATCCTTTTACAATGGAGCGAAAGGTTGATCATTTCCAAATGACATACCAAAGTTTTAAGGATTTATCAATTAAAGCTAAGTTGAGCTATACCTTTAATTGATTTGGAGATTATTCTTCAGGTGGTTTCACTGCTAAGAAAGGTGACAAGCATTATTTCGATCTTTTCCTTAAAATCAAACCTGATCCCAACAAATCATTTAAGGCTGCAAATTTTAAAACTGAAGAAAAAAACTCAACAGTTATTGATGGACAAGAAACTACAAGAAATTTAGAGTGAATTGAGTTTGGTGCTTCAATTAGCTGATCATTAAAAGGCAAAGATGATGCTAGTGAAAAAAGTGTGAAACAATTTTTAGATAGCTATGCTAATAACACATCAGGGTACTCATCAGATATTAATCTCTTTAGTTATTTGGAGTATTTAATTAGGTAATTTGTTGTGATTGCTTGGTTGTACTTGGAAAAATTTTAGCCACTTGCTTTTCGCCTTGAACAGATGGCTTTTTATTTTTATTCAAATTAATTTCATCAAAAACTTTTTTGCAATTTTGTTGACACTTCCAAAAGCTCATTTCAATTTATTTAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAGGAGGATTTGACCATTAAAAAAGTAAAGGTTACAGAGAAAGCTTCTGTTGAAAGAATGATAGAAGTATTAAGTGATTCTGAAATTTTATCTTATTTATTAAGTAATAAATACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAGCAGTATAAAATACCAGCAGTTAATGCATGGCTGTTGGTATTTTTATTCTTTCACTAAACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGTGATACCAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTAATGATGGGACGAGTTCGGATTCTAATAACCAAGATACGAATCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTAGAGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGCTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGTCCTTAAAAACAACCCCGGACCCAAAGTCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGATGGCAAGGGGAAGGATGAGAGCTCTTGGAACAGTTCGGAAAAAACAACGGCGGAAAGTGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATACCTCAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGGGCGGGGGAAAACAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCCACAAATGAAGATCTACCGAATGTAATCACCCAGTTATACCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCGAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGAAGGGGTACTTGATGAGCCCACACCGAGTTGAACTGGGGCACCGACAAGCAGAAGGAGTTTGTGGAGAATCAGCTTGGTTTCAATGAAACCAGCGCCACCGACTCGCATAACTTTAAAAAAGCGCTGTTACATCAACCCGCCTACCTCATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTCGAGCCGGCGCGGTGGGGTATGATATGCGACTGATACGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAACGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACGACTTATCCGGTCAAGTCCGACCAAAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATAAAAAAAGCCATCCACCTAAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACGATTGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGCCTGCTCCCACTAAATAAATTGAGAAAGATTTTCAAAGTAGTCAAAAAGTTTGAATATTTTGCAATTTGGGTTTGTAATCCCTTCTAACTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAAGACTCATAATGTTTAAATTAAAAATTAACAACTTCCAATTAGGTTTCAAACTCGTTCAGTGACCAGTGATGAACCACCTCCTTAAACATTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTCACCATTAAACGCACAATTTCACTTACTTTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGAATTTAATGGCAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAACGAATATAAAGACTCAAACGACCAACGGCTCATAAAGATTGAAACTACTTTGGCTGCCCAAGGTGAGCAAATTAGTCAGCTAGTTCAAATTGTGTTCCTCCAAGGAAAACAAATTAAAGAACTTCAAGCGGAGCAAAAAGCACAAAGAGTTGAATTTAACGCGCGCATGGATCGCTTTGAAAGCCTTGTGCTTAAGAGTCTGGAATCAATCGGAAACACTTTGACTGATTTTGGTAAACGACTCGATTCAATGGAAACTCGTTTGGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTAGACAAAATCGATCCACCTAAATAGTTTTAAGCTAAAAATACCAGCAGCTAAACTGTTTTTGGTATTTTTATTCACAAAAAAGCTAAAACGATTTAACGACCAAAATTGGTTTTAAAAGTAATTTCAAAAAAAATAATAACACACTGAGTAGAACCATTTAAACATTCAACTTTTAATTAATTAAAAACAGTCAAAATTTGCTTTTAAAAATACTTTTACTTCAGTAACTCAAACTAGTAAATTTCAAGGGTTAAACATCTACAAACTTTGTTAATATTTCTCTAAATTTCGGGTTTCTAGTGTTTATGAAATTTAAGTACGGCGCTATTGTTTTTAGTTGTCTTTTGGGCGTTTCGGCCATTTTAGCTGCCTGTGGGGCAAGGGGCAAATTTAACCAAGTTGATGATGGCAAGATTAAGCTAGCTTCATCTTTAACTTCCAGAAGTGCGTCAACAGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGCGTAAATGATTACCCCATTGAAATTACCCAAATTGCTGGCGGTTATGATGGCGGGCGGACTGATCTACAAACCCGAGTTAACGTTAAAGACACGACTAATTTTTATAACCTCATCTTGAACTATCCCGACTTAGTTTCCACTTTGGGACGTGTTGGGATGGAACTACCTTTTGATAAGGTTAAAACTGATAAGTTAGCTGACCGTTTTTTGGATTTCAACAACCGTATTAGTGCCATTTCCAAAAAGGGCATTTATGGCATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATTAATGGACCCGTTTTGCACTATATTTTGAATAGTGCACAAGGAAAGGTTAGTTCTGTCACTTCGGTGCAAAGAACAGCAGCTTCTGGTAGTGGAACAACAAATAATAATGGAGTAACAAAACCTTTGAAGATTGATAAAGAAAATGAATCTACAAAGAAACTTTGAGAAGAAATAGAAAACAAGGCAAAAGAAAACGGTAAGAAAACGAGTTCTTCAAGAAGAAAAAGAAATTTAAGTAGTTCTAAACAAATGAGCACTCAAACTCAACCTACTGACAATAGTAACGATGCTAATCAAAGCGACCAGGCGATAGAAAAGACTTGGGGTAAATATCAGGAGGTTGAAGGTGGTTTAAAAGATTACACCTTCAAAGCCAGTGTGTTTGAAAACTGACACGATTTGCTTGACTTTTCTAATAGGGTAGCTAAGTCATTTAGCAATATAAACACTAACACCAATAAAAAGGGCACCGATATTCAAGGTGTTCTGGGAATCGATAGCACTCCTAACTCCTTATTTACCTCTGTTTTTGCTGCAGGTGGCGGTAACTATGACAACTTCTTTTATAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCTTACCAAAACTTACAAAAAGTTTATGGTGATTACAAAGGCTTGATTGACAGCAATGGTCTCTTTGTTAATAAGGGTGGTTCTTATTCATCGAACTTCCAAAAGTTTCACCAATTAGCTTACAGTATTTCCTCGACTTCCGGCTTCTTCTACTCGTTTGCTGGTGAAAACTCAAAGCGCTTGAAGTTTGATGGCGGTAATTTTATTGAGTTTCCTGGATTTACTCAAGCAATTTATGCGCCTGAAAATAATAGTCAAAGTGGTGGAGAGAGTGGCGATCAAACCACAAATAATGAAGCCAATCTTTTAGGCACTTTCAAAATAAAAAGCTCTAATAAATCTAAATCTTCAAGTTCCAAATCAAGCACTAAAGCCGAAACAGGCAAAACTAGTGGTGGAGACCAAAACCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAACGGAGGTGAAGAAACAACTATTTACCTTTATAAAAACTCAATTCCATCTGGAAAAAATAAAGGCGAAAATGCAATTCTTATAGATAACAAAACTATTGTGGAACAACTAGAAAGTGCGGCTAAAAAAGAAGATAAGAAGAGTGGCGAATCTACCACAGAACAGACACAAATACAAAGTAAAAGTGTAACAGAACAAAAACAACCCAAAATCATCGGTTACACTACCACTGGCAATGTTCACGAAGACAACAAACACATTTTCCCAATTGACAAACTTACATCTGATCGAAATTTTGACCGCAAAATTATTGTTGGTGCCACTGAAGAAACACTCGATAAATCGAATACTTTGCAGTCCAATGAAGCCATTGTTTTACCAGCACCCTCTAAGTACAAAAGTACTGACACTAACAAAGTAACCATCACCCAAGGTCCGAATATCATTGGAATTCACGCCAACGAAAAGGAAAATGCAGAAACACAAAAGTTTGTGGATTGATTCTTAAACAGTCCGCAAACTTGAGAGGGTAAGGGAAAAGGTAAAGAACAAACTAATAAAACCGCAGCTGAATTCTTTGCTGAATCGGCTTCTTATATCTTGCCACTCAAGGAAATTTTTGAGCAAAAGAATGAAAAGAAAGAAGGCAGCGATCAAAAAGATAGTAAAAGCAATGGTAGAGGTAAACAAACTAACCTCTACACCGAAAAGGCCTTGGAACTATTTAGAGGTATTAGTACCGATCAAATAGTAAGTTACAGTGACCCAAGTGACTTTAGGAGTGGTCGTTTCCGTGATGGAATAGGTGCCACTTTTAACGCAGCAGTAAGCTCTAAAGCATCTTTTGAAAAGTTTGTTCAAAACTTTATTGCAACTTTAGGTAGTGATATTTAGCTAATCACTTTTGCAGTCCAGTCAAGGGTTTCATAAAGCTAGCGCTTTATAATCAAATAATGCTTTAAAGCAATTTAGACGACTCCATGAACGACTGACAGTGACTCAAAAACCGGCTGGTTAACGCCAAAACTAAGGCTGTTAGTTTCTGGCTAGCCCAAACTACCAGCACCATCTTAGATATTGCTGAACTAATTAAATGTTGTTCGGATTTAAAGAATACTTCCATTAATGGGTTAGTTGATCTTATAAATCAACAAGAAAAACTCGAATTTAACTTAACAAGGTTAAAGGAAATTGATGGCGAGGATGCACGACAGTTGTTTGGCATTGAAGGTAATGTTTACAAACACTTCCAAACTGAACTTAGTCGCTTTTACAAGCAAACGCGCAAACACTTTCGCGAAACTGGTTCGGAAAGTCTCTTTTTAGGTTTACCAGTAATTGAGGGCATTAACGAGTTTAACGATGTCTTTCGTGCTCCTTTACTCTATGTGGGGGTTAAGTTAAAAGTAGCACCACGACTTGAACGCTTTTGATTGGAAATAAACCGCGAGGAAATCTTTTTAAATCCGACCATCATCGGGGTTGAAATTAACAAGCGTAACAGCTTGTTTAAAAATAACTATGACACCACTAAGGTTGATATTAACCAGGCATTGGAAATCTTTCGTGAGTTGGAATACCAATTTCGGATGCCACTCACCTCGGAGTTAAAAAGTTTTTCCAAAAAGGCTAAGAGTGACTTTAACACTGAAAAACGGACTAACTTCCTCACTAATAACGTTCTTTTAGGAATCTTTGACGTTAAGGGGGATCAACTGTTCCAAAACTTTAACGAAATTCTAAACACTGATCCTGATGTTTTGGATGAATTGTTAAAGGATCGCCGCGATCTCTTATACGACAACCGTGAGTTTCGTGAAAACTTTAACTTAAAGGGCACTTACCTCTTTAGCCACTTAGACATCTTCCAGCAGTACGCTGTCAAACAGGCATTTGATGGGGATGTCATTATTGAAGGTCCACCTGGTACTGGTAAGAGTGAAACGATTGTCAACATCTTGGTCAACCTCGCTTTAAACAAAAAGAAGGTGTTGTTTGTGTCAGAAAAGGTAACTGCCTTAGATGTCGTTTACAACCGGCTAGGTAGCTTTAAGCACATTGCCTTATTTAATGCTTCGGTAGCGAGTGAGAAGAAGCGCTTTTACAGCCAGTTCGCTGATTATGAAAGCTTCTTTACCGATAACTTTTCCAAGAAGGATTTAGTTAACGAAATGCCGGTGTTTGACGGGCAGTGGGTTGATAAGATCCTAAGTGAATTCACTAACCTTCAAAACATTTACGATACCCAAATTAATTCGGGTAACCAGAGCTATTCCTTCAAGGAAATCCTAAGTAGTTTTCCAATACTTGATGTCAGTTACATTAAGATCAAGGAACACGATCGTTTTGATGAATGAGTGCGGGTATTTTCCTCACAAGTTTGATTAGAAAAACACTTAACTTACTTAGCGTTTAAAGCGGAACTGAGCAAACGCTGGCAAAACATCGATAACTTCTACGCTTTAAAAGACTTACTGGAAAAAAGAAAGAACATCCGCGTGTTGTGCTATGTGTTGGATTACTTCGAGCAGAACAACAGCATAATCAAACCGAAGCGGGTATTGCTCTATACCCCCACTGAACGCGGGCAAAAGCAGTTGCACCAACTCCAGCAAGACGTTGCTAAGTACAACAGCTTGCAACGTTTTAAATCGGCTGCTAAGTTTGAAACAATTAAGCTCAATTTAGCTAACAAACTAGCACAAAACGCCAAGCCTTTTTTCTTTTCTTGATTCATTCAAACACACGCGCAAACACTGTTGGAAAATCTCGTACAAACCCAAAAGCAATTGGTTAAGGCTAAGCAAAGCTACTTAAGCAAAATTGAGCAGTATGTAGTTAGTTGTAAACGGATTCTCAAAGCTACCATTTTAGCCAACTTCTTTGAGTTGTACCAAACCAATAAGAATGAACTATTGGATATCTGTCGGGAAGCCAAAAACCCTGTTTTAAAGGAAATTACCTGGTGGTTTAAGAAGAACTTTGCACTGTTATCAAAGCTATTCCCCGTCCACATTATGACGTTTGAATCAGCCGCCTTATTAACCCCGAACCAGCGCCGTTTGTATGACTATGTGGTGATTGATGAAGCTAGCCAGGTTTATCTAGAACGTGCCATTCCAATCTTATACCGTGGCGCTAAGTACATTATTGCTGGAGACACCAAACAATTAAAACCATCCAATTTCTTCCAAGCCCGGGCTGAGTATGATGTGGATGAAGAGTTTGAAGATGGTAACGTTGAAGCTGCGGTGCACTCGACTTCGTTACTACACTTTTTGAAGAACCGTTCGCGCATTTTAACGCTGCTCAAATTCCACTACCGCAGTGATTCCGCTAACCTAATTGCCTTTACTAATAACCGCATTTATAACAACGAGCTCATCTTTATGAATAAAGCAACCGCTGACAAGCAGGTCTTTATTGTCCATGATGTCATCGATGGTATTTGACGGAACAACCGTAACCTTCAAGAAGCACGCGATGTCGTACAACGCTTAGAACAGTTGACCCAAACTGCGGAATATCAAAAATCATTAGGGGTGATCTGTTTCAATAAAAATCAGGCCGAGTTAATTGAGTACATGATTGACAAGCAGAACAATCCGCTACTCAATGAGTGACGTGACCGGGTGAACGCTCAGGGCGAGTATGTCGGTTTGTTTGTCAAAAACATTGAAAACGTTCAGGGTGATGAACGGGACATTATTATCTTCTCGTTGGGCTATGACCGTTCTGTCAACAGCTATGGCCCGATTTCCAAGCAAGGTGGGGAGAACCGTTTGAATGTAGCGATTACCCGTGCTAAGCAGCGGATTGAACTGTTCAAGACTAACCGTGCTAGTGATTACAATGGTTTGAGCTCCAATTCCTTGGGTAGTAAGTTATTGGTGGAATACTTACTGTACTGTGAGGCAATGGCCAACAACCAGGGTGAGAGCTTAGACTTCCAAGCAACCCAAAAACAAGCACCCAAGGCCAAGTACGAATTGGAATTGGAGAACCAGTTCTTTAATGAGTTGGAACTTATTTTTGGTGAGCAGTTTACGATTAAACGCAATGTCAATGAGGGTGCATACAGCTTTAGCTTTGTCTTTTACTTTAACGAAAACCCTTACCTAGCGGTTGACTTTAACCCTGCTTTACCCCACTCCAGAAAGGAAGTGAGTGAAAACATTATTTACCGCGAACAATTTTTGAAAAAGCGGAAATGGAACCTCGTTAACATTTGGTTAGACGAGTGAAAACTCAATCCAGGTGGTGTGTTGCAAAAGCTTAGAAATTGTCTAACTCACAGCGAAAATGAGTTTGAAGAAATATAATTAAACGATATATTTCTCGTCATTTAAGAGAAGTGTCGCTAGGCACTTCTTTTTTATTTGCCCACTTTATCACATTATGAACAATCAATCAAATCACTTTACCAATCCTTTGTTACAACTGATTAAGAACGTTGCCGAGACCAAGAATTTAGCCATCGATGATGTTGTTTTGTGTCTTAAAACAGCATTAGAACAAACATACAAGAAGCATTTGAACTATGTCAATGTGGAAGTCAACATTGACTTTAACAAGGGCTTAATGCAAATTGAACAGCTCTTCGATGTTGTAGATGATAACAATGAGGATTATGATGACTTTTTGGAAATGCCTTTAAGTGAGGCTAAGAAGCTCAATCCGAACCTAGAAGTTGGCGGGGTGTTGCGCAAACCGGTTTCACTCAAAGACATTAAGGGCGATCTCATTAGCAAAATGGTATTACTCTTCAACCAAAAGATTAACGAAACAGCTTTTAAAACGGTGATGAGTGACTTCATTAATGAAGTCGGTCAGGTAATTGAAGCTAGAGTAGAGGACATTGATACGAACAAAGATGGTGGATTGAAGGGTTACATTGTTAACTTGGAAACCACCAAGGGTTACATGCCTAAACGTGAGTTGAGTAAGGGCGAAAAGCTAGATATTGGCAAGAAGTACCTCTTTGTGATTAAGGAAATCCAAAAGCAATCTTCGATGTGACCAATTACGCTTTCCAGGAGTGATTCGCGTTTACTGGAATTCTTGCTAAATTCCAACACTCCCGAAATTGCTAACGGTACGATCGAAATTAAAAAGATGGAACGCTCTCCTGGTACCAAGTCTAAGGTGGCCGTGATTTCCAAAGACCCAGTAGTTGATCCGATTGCGGCCATTTTGGGTCCCAAGGGCGAGCGCATTCGCGGCATTAGTGAAGAGTTTAACGGTGAGATCATTGATATTGTCATCTGAAATGAGGACAAGTTAAAGTTCTTGGTCAACGCTGTTTTACCGGCTGAAGTGGTCGGTTACAACATCCTTCAAGACGATGAGCGCGATACCAGCATTGAAATCGTGGTACCCGCCAACCAGATTGCCAACGTCTTTGGTTTTAAAGGAATTAACATTCGCTTAATTAGTAACCTAACGGGTTGGAGTAGTGTGGACGTTTACACCGAAAAAGATGCCGCTGAACAAGGGATTGAATACACCCGGGTCAACTTTCAACCCCAAGGTATTTTTGGCATCAAAAAGCGCCGCGACAAGATCTCCAACAATCCACGCAACAACAACCAACAGCTCGCATCTGATAAGGTGTTTTACACTTCGAAGGCCAATGTGGTTGATGATGAGATCATAGTCGATCTAGCAAAGCAAGCGGAAGCCAAACGGGTCAAGCAAATCAAGCAGGAAGCAACTAAACCCGAGTTGCAATTACAACAAGAACTTAACCTCGAAGCAACCCCTAAGGTAGCTGCTCCAACCCCAACCCCTGCACCCCAACCAACTCCCGCACCAACTAAAGTTGAGCCAGTACCACCACCAGTAAGCGTTACACCTAAGCCAATCCCCAAGGTCAATAAGCCCAAGCCAGTGGTTAAACCAAAATCAGTCTTTTCGATTACGGTTGAAGCGGATGACAGTAAGACTAAACCAGAAAAGTCAAGCGCTAAAACTAACACCCCACAAACGAAACAAACCTTTGATAACTTTGACGATCTCTAGTCGTTAATCTAACCATTTCACACTATGCAAAGTTGGCGCCAATGTTTAGTTACCAGAAAGTCTTTTGCTAAGCCGCTACTTTTGCGTCTTGTAAAATTAGAGCATCAACTACGGGTGGATTTAGCGCAAACACTACCAGGTCGGGGGTATTATTTAAGTGTAGCTGCTTTAAAACTGGAGCGGAAAAACTTTAAAGCACTGTTACAAAAACGCTTAAAGGTAAGCTGTTCTGACGCAGAGCTAGATCACATTATTGCTTGTTTAAAGGAAAGGGAAAGCGATGTCAAAGCATAAACCACGTCATTTTCAAAAGAATAAGTTTGACAACCGGGCTAAAACGAGTGCCAAACAACAGTTTCGCCAGGTAAAAACCGGTGTTAAAGATGGTGTCTTTGTCTATAAGGGACCACTCACTGTTAGTGAGTTTTGTCTCAAAACCAATATTCCGACGGCCAACATCATCAAGCACTTCTTTTTAAATGGGGTTCCCTTAACCTTAAATTCGGTGTTATCGACCGAACAACTAGCTGACGCTTGTGTTAACTTTGGCTTTGACTTTAAGGTAGAAACCGAAATCACCCACGACAACATTATCTCCAACATTAAGTTTGACGATGATCCCACCCAGTTAAGTCCAAGACCCCCGATCGTTACCATTATGGGTCACGTGGATCACGGGAAAACATCCTTGTTAGACGCCATTCGCCAAACTAATACAGCAGCCAAAGAGTTTGGTGGCATTACCCAAAAGATTGGGGCGTACCAGGTCAAAAACCAGGAGGGCAAAACGATTACCTTCATTGATACCCCCGGTCATGAAGCCTTTACCGGCATGCGCGCCCGTGGTGCTCAAGTGACTGACATTGTTGTCTTAGTGGTAGCGGGTGATGATGGCTTAAAACAACAAACTGAGGAAGCGATTTCCCACGCCAAGAGTGCCAAAACCCCGATCATTGTCTTTATTAACAAGATGGATAAACCAACCGCTAACCCCGACATGGTGATCCAACAGCTCAACAAGTTTGATTTAGTCCCTGAAGAATGGGGTGGTGATACCATCTTTGTCAAGGGCAGTGCCTTAACCAAAGAGGGGATCCAGGAGCTCTTGGACAGTATTCTGTTAGTAGCGGAAGTGGAAGATTACAAGGCCAACTTTAACGCCCACTCATCGGGTTATGCGATTGAGGTGCAAACCACCAAGGGGTTGGGTCCCACCGCTACCATTATTGTTAAACGTGGTACCCTAAAGATTGGTGACATCGTTGTCTTGGGTCCCGCTTGGGGTAAGGTTCGTACCATGCAAGACGAAAACGGAGTCCATCTCCAAGAAGCTATGCCCTCTAAACCAGTACAAATTTCGGGGTTTGACATTGTCCCAGTTGCTGGGGAAAAATTCATTGTCTTTGACGATGAAAAGGATGCGAAGTTAATTGCCAATAAGTTCCGCGAACAGCAAAAACAAAAGCTCAATACCACCCAAATTAACGAGGTGCTCAAACAAAAGATTAAGAGCAAGGAAATTAAGGTTTTAAATCTGATTTTTAAGGTTGACAGTGATGGTAGTTTAGCGGCCATTAAACAAGCTATGCAGAGCATTGATGTGCCGGGGATGAGCGTCAACATTATCCACTCAGGTGTTGGTTTAATCTCGGAAAACGACATTATGTTAGCCAAGGCGTCCGGGGCATTACTGTTTAGCTTGAACCTCGGTTTAAGTCAAGTTGTTAAGAACATTGCTAGTCTTCAAGGAGTGAAGGTCGATGTCCACTACCACATCCCTAAGTTAGCGGAAGAGATCGAAAACATCTTAAAGGGCCAGTTAGAACCAGTGTACGAAGACGTGGAACTGGGGCGTGCTGAAGTGTTACAACTCTGGTACCACTCCAAGGTGGGGCACATTGCTGGTACCTTAGTTAAAACAGGTAAGGTTAAACGGGGCGCCTTGTGTAAGCTGTTACGCCGTAACGAAACCATCTATGAAGGCCGGGTGGACTCATTGAAGAGTGAAAAGAACCCGGTCAACCAGATGGAAGCGGGGAAGAACTGCGGTATTGTTATTAATGGGTGTGAGGACATTCAGGTGTGCGACATTATCTTGGTGTACGAAAAGCAGGAGGTCAAGTCCAAAAGTTAACGATGGCTAGTTACAAAAAGGAACGTCTGGAAAACGACATTATTCGTTTAATTAACCGCACTGTTATTCACGAAATCTACAACGAAACGGTCAAAACCGGTCATGTAACCCACGTGAAACTATCGGATGATTTACTCCATGTAACAGTTTACCTAGACTGTTATAACCGTGAGCAAATTGACCGCGTGGTGGGTGCCTTTAACCAGGCCAAAGGGGTGTTTAGCCGCGTGTTAGCACACAATTTGTACTTAGCCAAAGCCGTGCAAATCCATTTTGTCAAGGACAAAGCAATTGATAACGCAATGCGCATTGAGTCTATCATCAACTCCCTTAAAAAATCAAAGCCTAATTAAAATTAATACTGTTTAATGAAACCACCTAAAAAGCCAGTCAACCCACCGTTTGGTAAGCTAACTGATTCTAAAGCTGGAGTAGTCAAAGCTACCTCTAGTCAAGAGACCAAAAAAGTAGCTGACACCAAACCCAAAAATAAGGGTGGTTTGTTTAGCTTCTTTAAAAAAGACAAAACGGAAAAACCAGCTAAAGCAGCCAAACCCAAAGATGCCTTTAAGAGTGCGATAGCTGAATTGAATCCGAAAGCCAATCCTAAGACAGTCAAAGCGGATGTTGCACCGGCTAAAATTCCCCACAGCGATAAGGGTACGGTTACCCCTGTAGAGTTAAAACCCCAAACGGAAGCACCGTTACCACCAGGAGTTAAACAACCTGATCCTAAAAAAGATAAACCTAAGGGCGGTTTATTTGGCTTCTTCAAAAAAGATAAGAACAAAGATGTCAAAAAGGAGCCTGCTAAGCCAGCTACTCCAGTAAAAACTGAACCAACCTCACCTAAGGTAGAACCAACTAAGGTCAAACCACCGGGCGGTGTACCGACTAAGCCGGTAGTGGAAAAACCAGTGTCAGCTCAACCAACGGTACCATTACAACCAGAACCTACTTTCCCAGTCAAAGCAGCACCACTACCACCAGGTGTAAAAGCAGAACCGGAAAGCAAAAAACGCTTTGGTTTGTTTAAGGCATTCCAGAAAGATGACGCTAAACAACCCAAGCAAAAGCTCAATCTCCAAGACCAACAGGATCGCTTTATTGATGACCCCACCGCCAAAAAGCACTTTAGCGCCTTTAACCAAAAAGTTGGTAACTTACTGAAGGACAAAAAAGACCCGTAACCGTGATTGGAAGATTGTGGGTTGAATCCACGGTTTAATCCTTCTGTTCTTCATTCCACTGTTAGCAATCATGAATAAATTTGTGACCCTCCCAGCACAAAGCTATCCTGCTGTATCACTACAAGTATCGATTAACAATGCGTTGTGGGGGATTGCGATCTTTGTGATAGCCAACATTGCTCTACCCTTCATTACGATGTTCGTGCTCTTCTTGACTGGGGTCAGAGATGTGCATGCTAGTCGGCCAGTGCACTATTTCATCTGGGTGTTAATGTTGTTAAACCTTACCTTCTTAGTAATTAGTTGTTGTTTACTGGCTGCTGCTTATGCTAACTTAGACATTTACAACATCTGACGCAACCTTCAGGCTTTAGACCCGAATAATTAGGGCTTGAGTTCTATGCAGCAAACCTTGATCATCGGTGCTTTTGATGGTTTACACAAGGGTCATCAATTGTTAGCACAAGCGGCTAGTGGTCCAGTGGTGGCCTTGTTAATTGCTAACATTCCCAGTCTACAAAGTGACTGGCTATACGAACCGAAGCAACGGCAAGTGCAGTTACAACAACACTTTCAGTCCGTGGTGCACAGCTATGATGTGATTGAACACAACATTAGTGCCCAAGCGTTTTTCGATCAAATCATTAGCCCACTACGGTGTCAGCAATTAGTCGTGGGTGCGGACTTTTGCTTTGGTAAAGACAACCAGAATGCTGATTTTTTAAGACGTTTATTTCCCAATACCACGATTATTCCCAAAGACAGTCAAACGCTGTCCAGTTCTACCATTCGCCAATGGTTAAAGCAGGGCCAGATAGAACAAGCCAACGCCGTGTTATTGGAACCCTACTTTAGGGAAGGGGTGGTTATTCGTGGTAACCAGCAAGCCCGGTTTTTGGGCTGACCAACCGCTAACATTACCTTAAAGCCGTACATGGTGCCGTTGCGCTGTGGGAGCTATGTGATTACGGTGACTTACCACCAAACCAATTACCCGGGTGTAGGGTTTATCAGTTACAAAAACGACCAGTTAGTCTGTGAAACCCACTTAATTGGTTTTAGTGGTGATCTTTACGGTAAACAACTACGCTTTACCTTCAACCAGTTTATTCGCCCCCAGCAAAAGTTTAGTGGGGTGCAAGCGTTGCAAAAAGCGATTAGTGGTGATTTAAAGCAAGCGCAGAAGTGGTTTGCTCAATCAACAAATTAAATAAAATTTAAACCTAATTTAACTTATGGAAAGTGCCCCCAGTGGTGGCCTGTTGGCCTTAATTATTATCAGTATTATCCTCCTGGCTTGTATTAGTGCCGTGGTTTCGGCTTACGAAACCGCCATTACCTCAATTACATCATACAAGTGGAGCAACTATGTCAAAACGCATAACAAGCAAAAGAAGTTAACGACCAAGATTGTTAACCACTTCCAAAAGAACTATTCGGCTTGTTTAATTACCATCTTAGTGGCCAATAACATTGTCGCCATTTTGGTTTCCAACATCCTCTTTTTGGCTTTAGATCAATCGATTAAGAATCCGGCCATTTCCAGTGCCTTGAACCTCTTAATTAGTGGGGTGTTATTGTTAATGCTGTGTGAAATTACTCCCAAAACCTTAGCACGGATTAACATCATCCGCGTCTTGGTCTACTTTGCGGTGGTAGTGTATTTTTTCTACATCCTTTTTTGACCAATTACCAAGTTAGCCAGCATTATCTTTGCCAAATACGAAAAGGCACCCCCAGTTTCCCGCCGTGATGTTTACTTCTTTATTGATGAAATTGAGCAAAACGGGTTATTTACCAAAGAAGACGGGCAGCTAATTAAACGGACCCTAATCTTTGACCAGGTCTTAGTTGACCAAATCATGATTAAGTGAAACCGGGTGGTGTACTGTTATGAAGGTGATCCAGTCAAGACCATTAAGGAAAAGTTCTTGCACGGCCAGTTTTCCAGAATGCCCGTACTCGACCAGACAAGCAATGAAGTGGTTGGTTTTATCCACCTCAAGGACTTGTTTAGTTCACTCGAAAAGAGCAACGAGCCGTTTGTTTTACAGGAGTTATTGTATCCAGCTGTTTTAGTAAGTAACACCACACCCATCAAGCAAGCTTTGCGCCAAATGCGGTTACACCGGGCCCACTTAGCGGTCGTGCAGGACAAACACCACCACACGATCGGGATTGTTTCTATGGAAGACATCATTGAGGAATTAGTCGGGGAAATTTACGATGAACATGATGAGGTGGAGGCAGTGCAAACGTTGGACAACAACACCTGGTTAGTGTTACCGAACGTAAAAGCCGCCTTTTTCTTTAACAAGTGGATTAAACGGGATTTAGTAAAATCAAAAAACATGACGATCCAACGTTACTTGGCATCGTTTGAAAATGATGGGTTAAACACCCAGAATAAACTAGAAACGCCGTGGTTTATTGCTGAAGCAATTGTCGACAGCGAGAACCCCGAGCAAATTCGGTACGAAATTCGCAAAAAGAGTGATGTTGTGGACTAGAGCCTTAATCTTAGAGTTAAAAACCAATAAGCAAAGCCGCTTGTTGTGGTTACTTGCAATTCCCTTACTAATATCGTTAACCTTACTAACGTACGGTTTAGTGTTGTTTAGTTCCAGTGGTCGAATTGATCATGGTGATCACTTCCACTTACGTGAGCGCTTTGTGTTAACGACCGAAGAGTTAGTTACCTTTGTGGTAGCGAGTGTCGTGTTTGCGCTAACTGTTGCCTTGTTTGGGCTGGGTTGCTGGAAGTTGCTCCAAGGTCCAAAAGTGGATAGAACAAACATAAAGCTAGCAAACAGCAATCCTGCTCCCCAAGCCGTGGTATTGCAAGCTGATTGTGATCACTTTCAGGTAGGGGATCACTGTGTGTTTAGCGCTGAAAAGCAACACTTTAAACAACAGTTCAAACAGGACTTTTTGGGTAAAAGTAAGTTTTCCTTTCGCAATGAACTGTACCGTTTTTGTCTCATTGGTGTGTTAATTTCGCTGAATTTAGCGCTCAGTATGGTGGAAATCCCCGGCATTGTTCTACCGTGGGGCAGTTCAATCCAGTTCCGCTTTTTTAACACCGCCATCTTGTTTATTGCGATTCGCTTTGTTGGGTTACTCTCCACATCGTTAATTGCGATTATTACTCCATGGATTCACCTCCTCCTTCACCCCGTTCATACCCCGATTAGTACCGTTTTTTACATGGGCAATGACTTGGTAGTTCTGTGAATATTCTATTTCTTTTACTACCACATCTTTAAGGCCGAAGTCAAACAAACCACTACGGTTGTCAATAACAAGGAGTTTAGTCAATTAGTCAACACCCACAAAACGAAGGTAGCCAAGGCCTTGGCGTTAATTCCAGTTAATTTAATCTGTGGTTTTATTGAGGGTTTGGGTTTCTATGTCGGTTACTTCTTGATTTTGGGTAAATTTGGTAGTGTAGGGCATAAGATTTTCTATGACAGTCAAGCTAACCGTGACCTAATTAACAGTGCTAACGTGATCTATTTCTTACTCACTACTACCACCATTTTTAGCTTAAAGTACCTGTTTGAACTACTGTTCTTTTACAGTGTCGAGAAGGGTATACTTAACATTTCGCGCCACTTTGGTTTGTATTAAAAGGTAAATAATTTAGCTTTTTGTTTAATCAGCAATTTCCTTTATATATAAAAAAGGTAATACAACGATAGTATAGGGGTTCTAGGGGAGTTAATTTGCAGAAAACAGCTAAACCATTGTTTCACCTGGCAAACAATTTAAAGTTAATGCCATATGAGTGACGTCAAATACTTACAGGTCGAAATTGTCGATAAAAACACCATTCGCCTCCAGGAAGATGGCAAAAAGGGTCAACAAATTAGGTTAGATCAAATTATTAAGGTTGATCAAAGCAACATCTTAAACACTTTGGAGCAGGCACAACGCGAGGCATACGAAAGGGAGGCGCAAAGCCGTTACCAAACTAAGTTGGCCAAAGAACTGAGTGAAAAAGACAACACTTTTTTAAAGCAAGAAGCAGCTTGAAACCAAGCGCACAACACGCAAATCCAACAGTTATACCAACAAATTACTAACTTAGAAAATCAGGTTAACAACATCAAAAGGGAAACTGAAAGTAAAAAGGATAACGAATATCAACAACAAATAGTTAAGTTAGAAACGCAGCTCCAAAGCATTAAAAAGGAGACCGAAAGTCAAAAAGACTTGGAATACGAACGTAAAGCAAATAAAACTAAGGAAGAAAATCAACAAGAATTAGAAAGACAACGCCAGTACTTTTTAGAACAGTTAGAACAAGCCCAAAAGGACATTGAAGAGCTCAAAACGCGTGAAGAGCGTTTTAGTAAGTGAGCAATTGCCAAAAAAGGGAAGGAATTGGAAAAGTGGTGTTGGGAAGCTTACAAAAATTACGAAGAGTTGTTCCAAAACTGTGTGTTTGCCCCTTACAGTGAGCTAGTTAAGGGTGCCAAAAAGAGCATAGGTGTAGAAGACGATGAGAGCAATATTAACGAAAAGGCTGACTTTATCTTTCAGGTATTTAATCCCAATAATGACAAAGAACCCTTCTTTTCAATTTGCTGTGAAATGAAAACAGAGTTTACTGAAAGCAAGAGTCGCACTAAAAACGAAGATCATGTCAAAAAACTGATTGCCGACGCTAAGCGCGCTAAGTGCCAATACGGTTTTTTAGTAAGTGAATTGGAACTCAACACTGAAAACGATGTCCAAGTGCAACGGATGCATACCTCGAACAGTGAAGTGGAGGTTTACCTAGTGCGCCCAATGTTCTTCATTGTGATGTTGCGGTTGTTTTACTTTTTGGCCAAAAAGATGTTTGCCCAAGTTGATGTAAACAGCGAGTACTTAGATAAAGAAGCTTTAAATGCTAGCTTTAGCGATTTAAAGAAAAGCTTACTTGAAAAAACCTTTACTGATCTCAACAAGGTCTTTCAAAACAACATTGATGAACTAGAAAAGATTGAAGGATTAGTAACAAAGTTGAAAGCCGCTAATGAAAAGGCCTTAAATAGTCGTTTAAATAACTGGGAAGAAAAGATCCGTAAGTTTGAATTTAAGCTCAATAAAGACATTGTTAAAAAGCTTGAATAAGGTTCTTTTAAGTAGTCTTTTTAGGGTAGAAATTAGTTTCCAAAAACGGTTTTAAATTAGAGGCTTATTTTTAAAAATTCAAAATAAGCAAATATAATTAAACCGTCTTTTATCTGAATATATGAAACTAAATCTGCGATTTCCTAGCTATTTTTTACCCGTCGTTGCCGCTAGTGCTTTCCTTGTTAGTTGCGCCACACCTAACACTTACGAAGTACAACGCGCTGCCTTGATTCAACTAGTTGAAGAAGACGAACGACAAAACTACATTCAAAAGGGTAGTATAGGTGCAAATGCTGTTATGACTGCTGCAAAAGCAGAAACCAAAACAGCAGAAAAAACAGCAACTTCTACCAAAGCTGCTTCAATAGAACTTAAAAAAACTGATACTGATATTAAGACTACAACTACTACCGAAAACAAATCAGCATCCGGATACAAACTAGATACCCTCTTTGGTGACTACATCTTGTGAGTAGTTGACCACCTTTCTGGTTTACTTTTCTCACCTAAGACTAATAACAGTACAACTACTCAAAAAATCCAACTCATTACCGAAGATAAGGTGATTTTAGACGGTGGTAACTTGACCGTGGAAAAAAATCATGAACATGGCCATACTCATAAAAATGGTGAAACTCACGAACATGATCATGATCACCACGAAGGTGAAGAGGAAGTAATCGTTGGTCGTGCCCTTTCTTTTGCTAATGGTCTCTTCTTAGTTGTGGATCTTAAGGAAGAAAAACACGAAGAGAAGAAGGAAGCAAAATCTGAAATGTCAATGAACAGTAAAGATATGGTGATGATGACCAAAACTGAAATGATGTCTAAGGAAATGAAATCCGAACAAAAAATGGAGAAGAAGGAGGAACACGAACATCATCACAAAAAGCTGAGCCTCAGTACCACGGCTTACAAGTTTGGTCAAAGCTTTAACATCCTGGAATTTACTGGTGCTATGCACCACAAAACGGCACACAATAACGAAACCGAATTTAAAAATTTAGGCAAAAAATACGGTGGTATGACCGAGTACACGATTGTCGACTTTGACTTTAATCCACCAAAACCAACTAAGTAAACGGCTTAACTAGCGCAAACAAGTCCCAATGCTTTTAGCTTGGAGCTTTTTTGCTGTTAGTAAGCTTCTTTTAACAATTAAATGGGTCTTTTAAAGTGACACAATAAGATCGCCAAAAAGCGGGGTTACTACTTTTTTGAAGTTGTTTAATTTCAATGATTATGGGAAAATAATAAGGCCTGCAAGCGGCAAACTAGTTTGGAAAAAGATAAGGAATTAGTCAGTTCAATTGTGACTGCTAAAAGCATGATTGACCGGTTTTTCTGGTCTATTTTGAGCTTTCTGCTCACTAACTTAGTCTTTTTATTTGTGGCTTTTTTAATTCTGATTATTTACTTAATTTCCGAAATCACCCAACAGTTTGCCTTTGCTTTTATCGCAACGATTGTCTTCATCATTTTTTACAACATCCTCTTTTTGAGCTACCTGTTGACGATGTACATTAAAGGCTTAAAACAGATTGAGCAAAAGTCACGTTATTTACTCTTAGTGCTTGATGTTAAGGCTGATGAACTGCTTCCTTTCTCCTTTTTAGGTAGTTTAAGAAAGAGTCACATGCTTGAAGAAATGTTGTTAGAGCAGTAATTATTTGCTACAATCTTAAGGCTTTGGTCGAAGTGGATACACCGACAAGCGTAGTCATTTTGACCATCAAATTTCACCCTTAAGAACTGAGATATGAACGCCGCTAACGCAGTTAAATACCCTGAGCTCAAGATTAAGCTCGAGTCCTATGACAGCACCCTTTTAGACCTCACTACGAAAAAGATAGTGGAGGTAGTAAAGGGTGTCGATGTCAAAATTAAAGGTCCATTGCCTCTACCTACCAAAAAAGAGGTAATTACGATCATCCGTTCACCCCATGTGGATAAAGCATCACGCGAACAGTTTGAAAAAACCGCCACAAACGTTTAATGATCTTGGTGGATGTTAACCAAGGTGCGATTGACAGCTTGAAGCGGATCAAGATCCCCGTTGGAGTAACTTTAAGATTTTCAAAGTAGTTAAAGAGAGAGTATGGAAATTCGTGGTATTTTTGGGGTTAAAGTAGGCATGAGCCAGGTGTTTACAACCAATAATGAGCGCCTACCTATTACCGTTATTTATTGTGAACCTAACCAGGTAGCAGGTGTTAAGAATGAGGCTAAGGACAAGTACAGTGCTACCTTGTTGAGTTTTGACACCGTTGAAAACAAAAAGCTCAACAAGCCTCAACAAGGCTTCTTTGAAAAAAACAACTTAAAGCCAACCAAGCACTTACAGGAAATCCGCAATATGACCGGGTTTGAAATGGGGCAACAAATCACACCGCAAAATCTCTTCCAAGTGGGTGAGTATGTTGATGTAAGTGCCATTAGTAAAGGTCGTGGGTTTACTGGTGCCATTAAGCGCTGGAACTTTAAGATCGGTCCGTTAGGTCATGGGGCTGGTTATCCCCACCGCTTCCAAGGTTCAGTCCAAGCTGGTCGTGGGGGTGCGAGTGCACAAAGAGTGTTTAAGGGCAAAAAGATGTCCGGACACTATGGTCATGAAAAAGTAACGGTACAAAACTTACGCATTGTTGGTTTTGATGAAGCTAACATGTTGGTATTAGTATCAGGGGCGATCGCAGGTCCTGAAGGTGGTGTGGTCTTCATCCGTACCGCTAAGAAAAAACCGGGGGTAGTGAAACCAATTGAGTTAGCTGTTCAAACCGAAAAGGCACCGGAAGCAAAACCGGCCAAGCTTTCCAAAAAGAAACAAGCCAAGGAGTTAGCGAAAGCACAAGCAGCTAACCAGCAAACTGTTGAAGCTAAGGTAGACACTCCAGTAGTAGAACCAAAACCAACTGAAGTTAAAAAAGCAGCACCAGTTGTAGAAAAGAAAGGGGAAGATAAATAATGGCTAAGCTTAAACTAATTAAAATCGACGGTTCCTTTGAAACTGAACCAGTGAAACTAAGCCCTGGTTTAATTGCTAAGGAACTCAAACAACAACCGGTGTTTGATGCCGTGTTAGTAGAACAAGCTTCCTGACGCCAAGGCACCCATTCCATTTTAACAAAGGGCGAAGTCCGTGGTGGTGGTAAGAAGCCTTACAAACAAAAACACACCGGGAAAGCGCGTCAAGGTTCAACCCGTAACCCCCACTTTGTTGGTGGTGGCATTGTGTTTGGTCCCAAGCCCAATCGTAACTACAGCTTAAAACTCAACAAGAAAGCGCACACGGCAGCCTTGCACACTGTTTGGAGTGAAAAGTTAGCTTCAGACAACACCCACCTAGTGGACCAAAACCTCTTTAACAAAACTGAGGGTAAAACCAAGGTGATGGTGCAATTTTTAAAGAGTGCCAAGCTCTTGGACAAAAACGTGTTGTTTGTAGTTAATACCCTTAACACCAACTTAGAACAAAGCACCAGCAACATTAAAAACGTGCAGGTCAAACACCTAGACAAGGTTTCTGTAAGGGATCTGATGTTAGCTAACGCTCTTTTAGTTGAAAAAGAAGTTTTAAAAGCTTTGGAGGGCAAATTTAAATAACCATGGATGTAACGAACGTACTGCTAAAACCAGTTCTAACCGAAAAGGTGTACTTCAACCAAATGGGTGAAACCAAGAAGTACGTCTTTGTGGTGAACCCTAAAGCAAGTAAAACACGCGTTAAACTAGCTTTTGAATTAGTGTATGGCATTAAGCCACTGAAGGTTAACACCTTAATTAGAAAACCAACCACCATTCGCGGTGGGAGCCGTTTTCCTGGTTTGAGCAAACTAGAAAAGTTGGCTGTGATTACCTTACCAAAGGGTATTGCCATTTCAGTTACTGGTGAAGCGCCAGAAAAAACCGATAAGCCAGCGGACAAAACCACCCTTAAGGAAAGTACTGTTAAGGAGATTAAGGACACCAAAAACAGTCCAGAAGCTGTTGTGAAAACTGCTGTGGAAGCGTTGCAAATTAAACCAACTGCCGCTCCTGTAACCACCGCCCCTCTACAAACCGTGGCGGTGAAGGTAGCTAAAGAAGTTAAAGAAGTTAAGGTAGAAAAGCCGGTTAAAGTGGAAAAACCAACCAAACCGGCAAAGGTAGCAAAAGAAGCTAAAACGACCAAAGTAGCTAAGGAAACTAAAGCTGAAAAGTCAGTTCAAACAACCAAAGTAGCTAAGGAAACTAAAACTGAAAAGTCAGCTAAAACTACCAAAACTACAGCTACAAAAACTACTAAAACTAAAACCACCAAAAAGGAGGTTAAAAAGTAATGCCTATTAAGAAAATTATTAGTCGTTCTAACAGTGGGATCCACCACTCCACGGTAATTGACTATAAGAAGTTATTAACCACCAATAAGAACAAACCAGAAAAGTCGTTGTTAGTTACCCTTAAAAAACACGGGGGTCGCAACAACCAAGGGAAAATTACGGTGCGCCACCAGGGTGGACGCAACAAGCGCAAGTACCGCATTATTGACTTTAAACGAACGCACTATGACAACATCGAAGCGACCGTTAAGTCGATCGAGTACGATCCGAACCGTTCTTGCTTTGTGTCGTTAATTACTTACGCTAACGGTGCCAAGAGTTACATCATTAGCCCTGATGGCATTAAGGTAGGTGATAAGATCTTGGCTTCCGAACACCCGATTGACATTAAACCGGGCTTTAGTATGCCCTTAGCCTTTATCCCTGAAGGTACCCAAGTGCATAACATTGAACTCCACCCTAAGGGAGGTGGTCAAATTGCCAGGAGTGCGGGGAGTTATGCCCGCATTTTGGGTCAAGACGAAACTGGTAAGTATGTCATTTTGCAACTTTTAAGTGGTGAAACACGCAAGTTCTTAAAGGAGTGCCGTGCTACTGTGGGAGTAGTTTCTAACTTAGACCACAACCTCGTAGTCATTGGTAAGGCTGGTCGCAACCGTCACCGCGGCATCCGTCCAACCGTTCGTGGTTCGGCCATGAACCCCAATGACCACCCCCACGGTGGTGGTGAAGGTCGTAGTCCCGTGGGTCGGGATGCACCCCGGACTCCATGGGGTAAGCGCCACATGGGTGTGAAGACCCGCAACATGAAGAAAGCATCCACTAACTTAATTATTCGTAACCGGAAAGGAGAACAATACTAATGTCACGCAGTGCTAAAAAAGGAGCCTTTGTTGATGCTCACCTCTTAAAGAAAGTGATCGATATGAACAAACAAGAGAAAAAACGTCCGATCAAAACCTGGTCCCGTCGTAGTACCATCTTCCCCGAATTTGTCGGTAATACCTTTGCTGTACATAACGGCAAGACCTTCATTAATGTTTATGTAACTGATGACATGGTGGGGCACAAACTCGGTGAGTTCTCCCCGACCCGTAACTTCAAACAACACACCGCTAACCGTTAGTATGATCGCCTTTGCAAAACAATTCCGTGTGAGAATCTCACCCCAAAAAGCCCGCTTAGTGTGTCAACTCATTGTGGGTAAGAAAACCGCTGATGCCCAAAACATCCTTTCCAATACACCCAAAAAAGCGGCTACCTTAATCGCTAAACTACTCAACAGTGCGATTGCCAACGCTACCAATAACCACGGCATGAATGGGGATGCCCTTTATGTCTTTGAGTGTGTGGCTAACCAAGGTCCGAGTATGAAGCGCACTATTCCCCGCGCTAAGGGCTCGAGTAACATGATTACCAAGCGCTCTTCTAATTTAGTGGTGAAACTATCGGATAACCCGAATGAACGTCAAGAATTAATTAAACAACAAAAGGCCCTAGTTAAAAAACGGGTCGAAGGTCAACAAAAAGCAAAGATGGCACGTCAAAAAGCAGTAACACCAGTGGTAAAAGCGCCAAGTAAAACTCAAGGAGGTGTACAAAAGTAATATGGGACAAAAAGTAAACTCCAACGGCTTGCGCTTTGGTATTAACAAAAACTGGATTTCGCGTTGAACCGCTAACTCCCACGCCCAGACTGCCAAGTGGTTAATTGAAGACGAAAAAATTCGTAACCTCTTCTTTGTCAACTACCGCAATGCCCAAGTTTCGAACGTGGAGATTGAACGTACTCAAGCAACCGTGGACGTATTTGTGTACGCCGCCCAACCCGCTTTTCTCATTGGTAGTGAAAACAAGAACATCCAAAAGATCACCAAGCAAATTAAGCAAATTATTGGTCGTACCACCAATTTAGACCTCACCATTAACGAAATTGGCTCCCCTATGTTATCAGCGCGCATTATTGCGCGGGATCTAGCCAACGCGATTGAAGCACGGGTACCGTTACGAACGGCAATGCGCCAATCCTTAATTAAGGTGTTGAAGGCTGGCGCTAACGGGATTAAGGTGTTGGTATCGGGTCGGTTAAACGGTGCCGAAATTGCGCGCGACAAGATGTACATCGAGGGCAATATGCCACTGTCAACCCTGCGTGCTGACATTGACTATGCCTTGGAAAAAGCGCAAACTACGTACGGTGTCATTGGTGTTAAGGTATGGATTAATCGCGGCATGATTTACACCAAGGGCTTAAACCGCACCCCGGCCCACATTCTCCACCCACAAAAGAAACAACCAAACCGCCAAAACCAACAACCACGCCACTTTAACCAGGGCCAGGTGTTGAGTGCTAATAAGTTAACTGGTAGTGATGTGGAGACCAGCAGTATCCAAGCACTTACTAAGCCTAACAAGGAGGACAAACAATAATGTTACAACCAAAAAGAACAAAGTACCGCAAACCTCACAACGTGAGTTACGAGGGCAAGGCTAAGGGCAACAGCTATGTTGCTTTTGGTGAATATGGTCTAGTAGCTACTAAGGGTAACTGGATCGATGCCCGTGCCATAGAATCAGCCCGGATTGCCATTAGTAAGTGTTTGGGTAAAACCGGTAAGATGTGGATCCGCATCTTCCCCCACATGTCCAAGACTAAAAAACCGTTAGAAGTACGGATGGGTTCTGGTAAGGGTAACCCGGAATTCTGGGTAGCAGTAGTAAAACAGGGCACGGTGATGTTTGAGGTAGCTAACATCCCTGAAAGCCAAATGATTAAGGCTTTAACCCGTGCTGGTCACAAGTTACCCGTGACCTGAAAGATTCTCAAACGCGAGGAGGTAAGTGCATAATGACCGTTGCAAAAGAATTACGCCAAAAGAGTTCCGAAGAGTTGGTAAAACTCGTGATTAAGCTCAAAGGGGAACTGTTAGAGTACCGCTTTAAGTTAGCACACGGGGAGCTAGACAAACCGCACTTAATTAACCAAACCAGGCGGTTATTGGCTACCATTTTAACGATTCTTACCGAGCGTAAGTTGAACTGACAAGAAGAACAAGCTAAGTACAAACTGCTCACTAAAAAAACGAACGAAGCAGCTGTGAATGCTTGGAAGCAACACCTAGAAGCTAACAAAGCGAAGCTGTTAAAGTCACGTGCTAAACGCGAGGATGCTAGCAAGAAATAATGAAACGTAATCAAAGAAAGGTCCTAATTGGGATTGTTAAAAGTACTAAAAACGCCAAGACCGCTACGGTTCAAGTAGAATCACGGTTTAAGCACCCTTTGTACCATAAGTCCGTAGTGCGACACAAGAAGTACCAAGCCCACAATGAGGGTGAAGTACTCGCTAAGGACGGGGACAAGGTACAAATTGTGGAAACGCGACCGTTGTCTGCAACCAAGCGCTTCCGCATTGCCAAAATTATTGAGAGGGTTAAATAGACTATGGTAAGTTTCATGACAAGACTAAATGTTGCCGATAACACGGGCGCCAAGCAAGTGGGCATCATTAAGGTATTAGGTTCCACCCGGAAACGTTATGCCTTTTTGGGTGATGTGGTAGTGGTATCAGTTAAGGATGCTATCCCTAGCGGTATGGTCAAAAAGGGTCAAGTGCTACGGGCAGTAATTGTCCGCACTAAAAAGGGCCAACAACGTAAGGATGGTACCCACCTTAAGTTTGATGACAACGCTTGTGTGTTAATTAAGGAAGACAAGTCACCAAGAGGCACACGGATCTTTGGTCCGGTAGCGCGTGAACTACGGGAACGTGGTTACAACAAAATTTTGAGTTTAGCAGTGGAGGTAGTGTAATGCAACGAATTAAAAAAGGTGATAAAGTAGTCGTAATTACCGGTAAGAATAAGGGTGGTAGTGGCATTGTTTTGAAAATTATGCCCGCGCGCCAACAAGCAATTGTAGAGGGACTCAACAAAGTAACACGCCACAAAAAGAAGGACCAAACCACAAAGCGCGCTGCTAAACAATCGACCGGTAAAGTGCAACAAGAAGCGCCAATCTTCTTGAGCAAGTTAGCTTTATTTGACCAAAAGGCAAAACAACAAACAATTGGCAAAATTAAGTACGTTATGGACCCTAAGACTAATAAGAAGACCCGGGTCTTTAAGAAATCCAACAACACGCTCTAGTTATGAATAATCTCAAAGCACATTACCAAAAAACAATTGCCAAAGAGCTGCAAAAGAGCTTTGCGTTTTCCTCGATTATGCAAGTACCGCGCTTGGAAAAAATTGTGATTAACATGGGGGTGGGTGACGTCATTCGCGACAGTAAGTTCTTGGAATCAGCCTTAAACGAACTCCACCTCATTAGCGGTCAAAAACCAGTTGCTACTAAGGCTAAAAACGCCATTTCCACCTATAAGCTGCGCGCTGGTCAACTAATCGGTTGTAAGGTTACCTTGCGCGGTGAGCGGATGTGGGCCTTTTTGGAAAAACTGATTTATGTTGCCCTACCTCGGGTGCGGGATTTCCGCGGCCTTTCTTTAAAGTCTTTTGATGGTCGGGGTAACTATACCATTGGAATTAAAGAACAAATTATTTTCCCGGAAATTGTCTATGACGACATTAAACGGATCCGCGGGTTTGACGTTACTCTGGTAACTTCAACCAATAAGGACAGCGAAGCATTAGCTTTACTTAGAGCGTTAAACCTCCCACTTGTAAAAGGATAATATGGCCAAAAAATCATTAAAAGTAAAACAAACTAGAATCCCCAAATTTGCCGTACGGGCTTACACCCGCTGTCAGCGTTGTGGTCGAGCCCGGGCAGTTTTAAGCCACTTTGGTGTGTGCCGTTTGTGCTTCCGCGAACTAGCATACGCTGGCGCAATTCCAGGAGTTAAAAAAGCATCATGATAACAACTACAAAACCAATTAAAGCCCACTTTGATCCAGTGGCTGACTTACTAACGAAGATTAACAATGCGCGCAAAGCGAAGTTAATGACGGTAACCACAATTGCTTCCAAGCTCAAGATTGCCATCTTGGAAATTTTGGTCAAGGAAGGTTATTTAGCTAACTTCCAGGTCTTGGAAAACAAGAGCAAGACCAAGCGGATTGTCACCTTTAACTTAAAATACACGCAGCGCCGGATTCCCTCCATTAACGGGGTGAAACAAATTTCCAAGCCGGGTCTGCGGATCTACCGGCCCTTTGAAAAGCTCCCGTTAGTTTTAAACGGTCTTGGGATTGCCATTATTTCCACTAGTGATGGCGTGATGACTGATAAGGTAGCGCGCTTAAAGAAAATTGGAGGGGAGATTCTCGCTTACGTTTGGTAAACAGTTATGTCAAAAATAGGAAACAGAACGATTACACTAGACCCCGCTAAGGTCAACCTTAACTTCCAGAAGGACCACATTGCCGTTAAGGGTCCTTTGGGTCAAATTGAGCTCAAACTACCCCCTAACTTACCGCTCAAGTTTGAACTGAAGGATAATAACCTCCAAATTACGCGGAACAACGAGTTAAAGCAATCCAAGATCTTCCATGGTACTTACAACGCGTTAATTACCAACGCCATTATTGGGGTTACTCAAGGCTTTGAAAAGAAGCTGCGCTTAGTCGGTGTGGGTTACCGTGCTAACGTGGAAGGGGAAACGCTCAACCTCCAGTTGGGTTACTCCCATCCCATTAAGGAAAAGATTCCCAAAGGCTTAACAGTTAAAGTAGAAAAGAACACCGAGATTACCATTTCGGGAATTAGTAAGGAGTTAGTGGGTCAGTTTGCCACTGAGGTGCGCAAGTGACGCAAACCCGAACCGTATAAGGGTAAGGGAGTGTTGTACTTTGATGAAGTGATTGTCCGTAAAGCTGGTAAAACCGCGGAGGGTAAAAAATAGATGAAAACACGAACAGAACAACGGCGCTTACGCCATAAAAGGATTGTGAAGAAGATCCGCGCCACTAACCACGACAACCGCGTTGTGTTAATGGTGATTAAGTCCTTAAACCATATTAGTGTGCAAGCGTGGGACTTTTCCCAGAACATTGTTTTAGCGTCCTCTTCCTCACTTGCTTTAAAATTAAAGAATGGTAACAAGGACAATGCCAAGTTAGTGGGGCAAGACATTGCTGATAAACTAGTGAAGCTCAAACTAACCAACGTTGTCTTTGACACTGGTGGTAGTAAGTACCACGGCCGGATTGCGGCATTGGCCGAAGCAGCCCGCGAAAGGGGGTTAAACTTCTAATTTATGACTGATCAAAACCAAAAAGCAAATCAAGGTAACGGCTTGCAAACCACTAACCTCCAAGCCCACGCACAGCGTAAACACAACCTCCGTCCTTCCTCTGAAGGGATTAAGAAAGCCGTGTCCAAAAAGGAAGGTGGTGGTCACAACCGTAACAACCAAAACCGTCGCTTCCAAAAACCGGCTTTCAAGAGTGAGTTTGAAGAACGCATCGTCAAACTAAAGCGCATTTCTAAAACCACTAAGGGTGGACGGAACATGCGGTTTAGTGTGCTGGTCGTAGTTGGTAACCGCAAGGGCAAGATCGGTTATGGGATTGCTAAGGCCTTGGAAGTACCGAATGCGATTAAGAAAGCCATTAAAGCAGCGCACAACTCACTCCACACAATTGAAATCCACAAGGGTTCAATTTACCACGAAGTAATTGGCCGTAGTGGGGCGAGTCGCGTGTTGCTCAAACCCGCTCCTCAAGGTACGGGAATTATTGCTGGTGGTGCCATTCGGGCCATTATTGAGCTCGCTGGTTACAGTGACATTTACACCAAGAACCTCGGTCGCAACACGCCAATTAACATGATCCATGCCACCATGGATGGGATCTTAAAGCAGCTCTCTCCACGTCGGGTAGCGATCTTACGTAACAAAAACCTCAACGAACTTTAGACAATGGAACTCAATCAACTCAAAAGTGTCCCCAAAGCGCGAAACCACAAGACCAAAACTTTAGGTCGTGGGCACGGCTCGGGTTTAGGTAAAACATCTGGTCGAGGCCAGAAGGGGCAAAAAGCGCGCAAGTCCGGTTTAACCCGTCCTGGTTTTGAAGGAGGACAAACGCCACTGTACCGTCGTTTACCGAAGTTTGGTAACGCACGCAAGGGCTTTTTAAAGCAGGAGTGGGTGGTGTTAAACCTCAATAAAATTGCTAAATTAAAGCTCGACAAAATTAACCGCGCTAGCTTAATTGAAAAGCAAGTAATTAGCGCAAAGAGCCAATTACCAATTAAACTAATTGGCAACACTAAGCTCGAAAAGCCGTTGCACTTTGAGGTGCACAAGGTTTCCAAGCAAGCTTTGAAAGCGGTTGAAAACGCCAACGGTAGTGTGAAGCTGTTAGAGAAATAATGCAAGCCAAACCAACAACTGCAGTTAAACAAAAACAGAATTTTGGGCAACGTTTGTTCACTTTGTTGCGCAACCGTGACTTCATGATCTCGTTTTTAATAACGGTCGTGCTGTTGGTTTTGTTTCGTGTTTTAGCGATTATTCCGCTCCCCGGCATCCAAGTCAACCAAACCGGGCTAGACCAAAACTCAAACGATTTCTTCTCACTGTTTAACCTACTTGGTGGGGGTGGATTGAACCAACTATCGCTGTTTGCGGTGGGAATTAGTCCGTATATTTCGGCGCAAATTATTATGCAGCTGTTGTCAACGGACTTAATTCCCCCGTTATCTAAACTGGTGAACAGTGGGGAAGTGGGTCGGCGTAAAATCGAGATGATTACGCGGATTATTACGCTACCGTTTGCGTTGGTGCAATCGTTTGCTGTGATCCAAATTGCCACTAATTCCGGTGGCGGTTCTTCACCTATTACCTTAAAAAACAACGGTTCGGATTTTGTCGCGTTTTACATCATTGCCATGACTGCTGGCACCTACCTGTCGGTCTTTTTAGGTGACACCATTTCCAAAAAGGGAATTGGTAACGGGATCACCCTGTTAATTCTTTCGGGGATTTTAGCCCAGCTACCCGAAGGATTTATAGCTGCTTACAGCGTGTTATCAGGTGTAGTGGTAACCATTAACGCAACCTTAACCACTGCCATTAGCTTCTTCATTTACTTCATGGCGTTTTTGGCATTACTGTTTGCCACTACCTTTATTACCCAAGCGACCCGGAAGATTCCGATTCAACAATCCGGTCAGGGTTTAGTAACCGAATCCTCTGCGTTACCTTACCTCCCAATTAAGGTCAACTCCGCCGGGGTAATTCCGGTGATCTTTGCGTCTTCGATTATGTCAATTCCGGTGACGATTGCCCAGTTCCAACCGCAAACCGAATCACGCTGGTTTGTGGAGGACTATCTTTCACTGTCCAAACCAACTGGCATTGTCCTGTATGGTATCTTGGTGATTCTCTTTAGCTTCTTTTACAGCTACATCCAGATCAATCCAGAACGGTTAGCCAAAAACTTCGAGAAGTCCGGGCGTTTTATCCCGGGTATTCGTCCCGGTAAGGATACTGAGAAACACATTGCCAGAGTACTCGTGCGGATTAACTTTATTGGCGCCCCTTTCCTAACGGTGATTGCCATTATTCCTTACATAGTGTCAGCCTTAATCCACTTACCCAACTCCTTAAGTTTAGGGGGTACGGGGATTATTATCATTGTTACCGCTGTGGTTGAGTTTATGTCAGCACTCCGCTCTGCAGCCACCGCTACTAACTACCAACAACTGCGCCGCAACTTGGCGATTGAAGTGCAAAAAACAGCCCAACAAGACAAGGAGGAACAACTGCGCGCTGAAACCCCTGGTATTGGTAACCTCTGGTAATGGAAAATAAGTTCTTGTTTTTAGGCGCTCCGGGTGTGGGTAAGGGTACACTAGCTAAGCAAGTAGCCAACACCACCGGATTGTTTCACCTTTCCACTGGTGACATCTTTCGCAGTGTGATGCAAGAGCAGGGTGCGTTGAGTCAAACGCTAGCGCACTATATGAACCAGGGTTTGTATGTCCCTGATGAATTGACTAACCAAACCTTTTGGCACTTTGTCACTACCCACCAAAATGAACTCCACAAGGGCTTTATCCTAGATGGGTATCCCCGTACCTTAAACCAACTCGAGTTTTTGCAAAGCAAGTTGCAATTAGACCAGGTGTTTCACCTTAAGCTAAGTGATCCCCAAGTGCTAGTAGCGCGGATCTTAAACCGTTTGGTTTGTCCTAGTTGTGGTAGTGTATACAACAAGCAGAGCAAACCACCATTGAAGGCCAATCAGTGTGATCGTTGCCATGCTACGTTACAAGCGCGCAATGATGACACGGAAGCGGTAATCTTAAAGCGTTTAACGCTTTACGAAGACACCGTTAAACCCTTAATTGAGGCCTTCACCAAGCAGGGCATTCTCACCGTCATTGAAGCCCAATTACCGCTCGAACAACAGGTCAATTTGGTTCAACAGTTAGTGCATTAATGGTTTATTTAAAGTCTGCGCGTGAGGTCGAACAGATCCGCCAAGCATGTAAGATTTTCCAAGAAGCAAAGGCATATTTTACAATTGAAAGGCTACTTGGCAAATCTTTAACAGCAATTGATCAAGCGTTAAAGCAGTTTATTGAGAGCAAAGGTGCAACTTGTGCCTTTCATAAATACCAAAATTTCCCGGGATTTAACTGCCTTTCGTTAAACGAAACGGTAATCCATGGCATAGCGGACAACCGTGTTTTTGGTGTTAAGGATAAACTGACGTTGGATATTGGCATTAACCTTAACGGTTATATTTGCGATGCAGCTTTTACGGTTTTGGGCCCCAAGGCTCCAGAGCCCATGCAAACGCTGTTAGAGGTAACGGAAGCTTGTTTTACTGCTGTGGTGGAGCCTCAACTGCGCCCCAATAACCCCACTGGTAATGTTTCCCATGCCATCCAAACCTACTTTGAATCTAAGGGTTATTACCTTTTAAAACAGTTTGGTGGTCATGGTTGTGGGATTAAGGTGCACGAAGAACCGCTGATTTTGAACTATGGTAAGCCTGATACAGGTACTAAGTTAGAACCAGGGATGGTCTTGTGCATTGAGCCCATGGTAATGACTGACAGTGATGCAATGGTAATGCATAACAACAGCTGGAATGTATTAACCCCTAAAAGCCGTTACAATTGTCATGTAGAACAGATGTATGTGATCACCACAAGTGGATTTGAATGTTTAACTAATTAATGCAACCAAAGTTTAATAACCAAGCAAAGCAGGACAAACTCGTCTTAACGGGCAAAATCTTGGAAATCATCCACGGTGACAAGTTTCGCGTGTTGTTGGAAAACAACGTTGAAGTAGATGCTCACTTAGCGGGCAAAATGCGAATGCGCCGGTTACGAATTTTACCGGGCGATTTAGTTGAAGTGGAATTTTCCCCTTATGATTTAAAGTTAGGTCGAATAATAGGTAGAAAATAATTAAAATACTATGAAAGTTAGAGCGAGCGTAAAGCCAATTTGCAAAGATTGCAAGATCATTAAGCGCCACCAAATTGTGCGCGTTATTTGCAAAACTCAAAAACATAAACAACGACAAGGATAATGGCACGGATTTTAGGTATTGACATTCCCAACCAGAAACGCATAGAAATTGCGTTAACCTATATTTTTGGTATAGGGCTTTCGCGCTCCCAAGCCATCTTAAAGCAAGCGAACATTAACCCCGATAAACGGGTCAAAGACTTAACCGAAGAGGAGTTTGTTGCCATTAGGAATGTAGCTAGTGCTTACAAGATTGAGGGTGATTTGCGTCGTGAAATTGCACTCAACATTAAGCATTTGAGTGAAATTGGAGCATGAAGGGGCTTGCGCCACCGCAAGAACTTACCAGTGCGCGGACAACGTACCCGTACCAATGCCCGCACCAGAAAGGGTCCACGTAAGACCGTGGCTAACAAGAAGATTGAAAGCAAGTAATGGCTAAGAAGAAAAAGATTAATGTATCTAGTGGAATTATCCACGTATCCTGTTCCCCAAATAACACAATTGTATCCGCTAGTGATCCGGGGGGCAACGTGTTGTGCTGAGCTAGCAGTGGTACCATGGGCTTTAAGGGATCGCGCAAAAAGACCCCGTACTCAGCCGGCATAGCAGCCGATAAGGTGGCCAAAACCGTCAAGGAAATGGGGATGGCGACCGTTAAGCTCTTTGTCAAGGGTACAGGTCGGGGTAAGGATACCGCCATTCGCAGCTTTGCTAATGCGGGACTTTCTATTACTGAAATTAACGAAAAGACCCCGATTCCCCACAACGGTTGCAAACCACCAAAACGACCACGCTAAGATTTATGGAAAAGTTTTTAAAGTACGAAATAAAGGTAAATAACGAACAAGCACGCGCTAACCCCAACTACGGCATCTTTGAAGTTGGACCACTCGAATCGGGCTTTGGCATTACCATCGGTAATGCTATGCGCCGGGTGCTGTTGAGCTGTATTCCAGGTGCCAGTGTGTTTGCACTCTCAATTTCGGGTGCCAAGCAAGAGTTTGCTGCTGTGGAAGGGATGAAGGAAGATGTCACTGAAGTGGTGTTAAACTTCAAGCAATTGGTAGTGAAGATTTCTGACCTTCTGTTTGAAGATGGTGAAATGGTCGAACCACCACTAGAACGTTGACCATTGTTAACGGTAACAGCTGAGAAAGCGGGACCGGTGTATGCGAAGGACTTGGAGTGCCCAGCTGGCTTTGAAGTGGTCAACAAGGACTTGTACCTGTTCTCGTTGCAAACTGACAAAAAGGTAACAGTTAACGTGTATGTGAAACAAGGACGGGGGTTTGTTACTTTCTTGGAAAACCGTGAAATGATTAATTCTTTGGGGATTATTGCCACTGACTCGAACTTCTCCCCGGTATTACACTGTGGTTATGAAGTACAGGAATTGAAAACTTCCAAGCAAAAGATTACTGATCACTTAACTTTCAAAATTGCTACTAACGGTGCCATATCCGCTGTGGATGCCTTTGCAATGGCTGCAAAGATCTTGATCGAACACCTCAATCCGATTGTTAATGTCAACGAATCGATTAAGGCGTTGAATATTATCCAAGAGAAAGCCGAAGAACGCCGTGTCCGTTCGTTTGCGAAGCAAATTGAAGAGTTGGACTTTACCGTGCGTACCTTTAACTGTTTGAAGCGTAGTGGGATCCACACCTTACAAGAGTTGTTGTCCAAGTCATTAGCTGATATTCGTGAAATCCGTAACCTCGGTAAGAAATCGGAACGCGAAATTATTAAGAAGGTACACGAATTAGGACTCAAATTAAGATCTTAGTTATGTCATACATTAATAAACCAGGTAAAACTAGTGCGTGAAGGGTCATGACCGTGCGCCAACAGGTAAGCGCTGTTTTGGCTTATGGCAAGATTGAAACAACCTTAAAGAAGGCCAAGAACACCCAAAAGCGTTTAGATAAGTTAATTACCTTAGCTAAAGTTGATAACTTTAATAACCGTCGTCAAGTCAAAAAGTGGTTGTTAAACACCAACTTGTTCGATGTTGATCAACTAATGGATCACTTGTTTAGCAAAGTTGCCCCTAAATACGAAAAAACCCCCGGTGGTTACAGCCGTGTTTTAAAATTAGGGCCACGTCGCGGAGATGCCACGGAAATGGCCATCTTGCAACTAACTGATGCTAAATACAAATAAACAAGCAGCTGCAAGCTTTCAAGGTGTTTATTTTTCTTACAGCGAATTACCATTAATTCGCAACCTCTCCTTTGATGTTTTCGAAGGGGAGTATGTTTGCATAGTTGGCCATAACGGCTCGGGTAAATCCACCATCTCGAAGCTGTTAACGGGCTTGTTAAAACCACAAGCGGGTACCATCAAAATCTTTGGTAAGACGATTTCGAGTGAAAACGTGACTTACCTACGCAACCACATTGGCATTATCTTCCAAAACCCCGATAACCAGTTTATTGGTATTACCGTGGAAGATGACATTGCTTTTGGACTGGAAAACCGCCGTTATACGCGCGAAAAGATGAAGAGCATCATTGAGGATGTAGCCCAACAAGCGGGAATAACGGAGTTATTGCAAAAAGAACCGCATAACCTTTCGGGTGGGCAAAAGCAACGCGTTGCCATTGCCTCGGTGTTAGCCTTAAACCCAGCCATCATTATCTTTGATGAGTCGACTTCGATGTTGGATCCTAAAGCGAAGCGTACCATTAAGCAGTTCATGGTGGAACTGCGCAACCAGGGCAAGTGTGTTATCTCCATTACCCATGACATGGAAGAGGTTACCAAAGCAGACAAGGTTTTGGTCATGAATGAAGGTCGTTTAATTCGTCAAGGTAAACCCAAGGATGTCTTTAACAGTGCAGCCGAATTGCAAAAAATCCGCTTGGATATTCCCTTTTCTTTAAGCCTTTCCACGAAGGTTAATGGTGTGACTAGTACAATAGACTACCAACAACTAATTGAGGCCATTGGTAAGCTATGAAAAAAATAGTTGAACTCAAAAACCCCTTAAGGGATGGAGAAATCCTCAGTGTTAGTAACCTTTCGTGCTTTTTTAACGAAAAAACAGTCCATGAAGTCAAGGTAATTGACAACTTTAGCTATACCTTTGCTAAAAATAAGGTCTACTGCATTATTGGTGACAGTGGTAGTGGTAAATCAACCTTAGTCAACCATTTTAATGGTTTAATAAAGCCCGCTTACGGAGATATTTGGGTCAAGGATATTTACATTGGTCAAAAGCAACGCAAAATTAAGGACTTTAAACGCTTGCGCAAAACAATTTCGATTGTCTTTCAGTTTCCCGAATACCAACTGTTTAAGGACACAGTGGAAAAGGACATTATGTTCGGTCCAATTGCTTTGGGTCAATCGAAAAACGAAGCACGCCAAAAGGCAGCTTACTACCTCGAAAAAATGGGGTTAAAGTACACTTTTTTAGAGCGCAATCCGTTTGAGTTGAGCGGGGGGCAAAAGCGCCGTGTAGCGATTGCTGGGATTTTAGCAATTGAACCCGAAGTTTTAATCTTTGATGAACCTACCGCTGGACTAGATCCTGAAGGGGAACGAGAAATGATGCGTTTAATTAAGGATGCTAAAGCTAGTGGTCGCACCGTCTTTATGATTACCCACCAAATGGAAAATGTTTTGGAAGTAGCGGATGAAGTGCTGGTGTTGTCCAAGGGCCAACTCGTTAAATCCGGTGATCCTTACGAAGTTTTTATGGATGAAGCATTTTTGGCACACACCACCATTATTATGCCTCCAGTCATTCAAGTTATCAAAGACCTAATTAACTTAAACCCGAAGTTTAGTTGTTTATTAGACTTTAAACCGCGCAACTTAGACCAGTTGGCGGATGCCATTAACAACACAATAGCTCATGGATAGTTTTTTTATTAATGGTTATGTACCACGAGATACCTTTATCCACCGGCTCCACCCGACAACGAAACTGTTAATCTTTTTGCTGTTCGTGATCCTGGTGTTTGTACCCATTGGCTTTGTTTTTCAAAGTGTGATCTTTGTCTTTGCCACAGTTATTTTCTTTGTAGCCAAACTACCCGGACGGTTTTACCTCTCCTCGATTAAGTCAATCTCACTGTTATTCTTACTACTGTTATTTGTCAACTGGTTTACCTTCCGTGACCCCGGGTTCTACATTACCGCTGATCAGGTTAATACAGTAAAACCACACTTTAACGGTAATAACTTTAACTTCTGAAACATCTCCCTGTTTAACTATCAGGACAATGTGTTTTCGCAAGTGTTTAACTTTAACCGTGCCAACATGACAGAGTTAAACAAGATTAACTTCTTCTTTAAGGAAACAGCTAATGCTAATGCATACACCAAGGTAACGGGGATTGATAAATTGGCAGAAATGTTGGCTTCAAAGAATCTCTTTAAGTTCAACGGTTCTACCAATGGCATTGATAAAAACAAGATTCTTGGTGCCTTTCTAGATCATAAGATTGCCGTTTACCTCGGTCGTAGTTGGGGTGGTGACTTTAGTGGTTTTGTGATTGACGTAAGTGTAAGTGATAAAACATCTACCTTCACGATTAAACCCTTTTTAGCTAACTCGAACTATGTGTTGACACTGCGTGCCATTATTCTAGCCTTTTACGTTACCCAAAAGATCTTGATCATGATCATTTTGGCAACAGTGTTAACTTCCACTTCAAGCTCGGTTGAGTTGGCATACGGAATTGAACGCTTGCTGTGACCGTTGAAGCTGTTACGGGTTCCAGTCAATGTGTTTGCGATGACAATTGCAATTGCAATTCGCTTTGTCCCTTCTTTATTGTTGGAATCACAACGGATTTTAAATGCCCAAGCATCACGCGGTTTGGACTTTAAGAACGGCAATTTCTTTGTCAAAATGCGTTCATTGTCTTCCTTGGTTGTACCGATGATTTCGATTGCATTCCGCAATGCTGGGGAGTTGGCGAGTGCCATGGAAGCACGTGGTTATGATCCAACTAAGAAACGTACTACCTACCGCAAGTTTAAGATTGATTGGGTTGATGCCACAGCTTTAATTCTCACCGCTCTTTACTTTGTGGTGATTATTTTCTTAACCGTTAAGGGTGCAGTGTTCCTAGATTTAGGGACACCTGAGTGGTTGCTAACCGGCAAGATTAAAGAGCAGGTAGAGCGCTCATTATCAGTTAAAAGTGCCTAGGTACTTAGCGATTGTCGCTTACAATGGTGCTCCCTTTCGTGGTTGAGCCAAACAACCGGGTTACCACACCGTTCAGGGTCAAATAGAACAAAACCTAGCCTTAATTTACCAAACACCAATTAATATTTATGGTTCGGGTCGGACTGATAAGGGAGTGCATGCCATTAACCAAACCTTTCACGTTGATCTACCTGATAAGTTACCACTCAAACAGTTAATTGCCAAACTAAACCAATTAAACTACCCTGACATTTTTGTAAAGGCCTTAGTTCCAGTAGCACCGCGATTTCACGCGCGGTTTGCAGTAAAAACTAAGGTTTATGAATACCTTATTAACACCCGAGCTTTTAACCCAGCACAGTACCACACCGTGTGACAGTACAACCACCCATTGGATTTAGCACAACTTGAAGCGGATACCACGCTCTTTTTAGGCAGGAAGGATTTTTGGAGTTTTAGCACCGCTAGCCAAAAGGACACGCAGCGCACAATTAGTAAAATTATCGTGCAACAAGATCACACTGGTACGGTTAAACTGACCTTTTTTGGCAACGGTTTTTTAAGAAACCAGATTCGAATGATGGTAGCTTCTCTAGTGGCCTTAAACAATCGAACCCTGATGCGAGAAACAGTTCAACAGCTGTTTGATCAACCACAAAAGGGTGGGTGTAAGGTTAAAGCCCCCGCTTGTGGTCTTTATTTAAAAACTGTCTTATATGAATAACCAATACAACTGAAACCTCGAAGTTTTACTAAATGGTAAGAGTTTAGCTGATAACTTTACCGAACTCAAACAACTCAGTGAGCAGGAAAAAGCACTTTACGATGGTGGTGCTTGCTTTCAAACCAAAGCTAAGTTCACCGAATTTTTACAACTACAAGAAAAGATTGAGGTCTTAGAAAACCGTTACAGCAATTTTTTGAGTAACAAGCACGCTGAAAACAGTTTGGACAAAACGATTAACGACGCTTTATTTCAGTACGAAATGTTTAAAAGTGAACATGCCTTGGTGTTTGTTGACTTTGAAAAGAACCTGTTTAAGCACGAAAAGGTGATTCGCGCATACCTCCAAGACCCTGCTTTAAAACAATACCAAAGGGATTTTGAACTAGTATGACGGAACAAGAAACACCAAATTGATCCCGCTAGTCAAAAGTTGTTAGCGCAAATTAGTCCGGCATTAAACCAAGCGGACAAGATCTTTAATGTGTTATCTACCGCTGACCTAAACCTCCAACCAGTCGTATACAAGGGTAAAACTTACGTTATTAACGCGGTTAGTGATTATCAAAGTCTCTTGGAAAATAAGGATCGTGGCCTGCGTGAAGCTGCTTACAAAGTGTGGTTGGAAATTTACTGACCCACACGCAATACCTTGAGTGTGAGTTTAGTGGAAAACTACATCCAATTAGAAACCTTTGCTAAGTTAAAGAAACACCCCAATTACATTGCCAAAACAGCTTTTGATGATGAAATTGATGTGGCTTTCATTGACTTTGTTTATGAGCAAGTGGCTAGCTTTGCCCCGACCTTTAAAGCCTTTCAAAGTTTAAGGAAGCAAATTTACAAGCACGTTTTAAAACTAGACAAAGCCCAACCTTGGGACTTGTCGGTACCGTTGTTTAAGGCTAGTGGTGACTACACCATTGAACAAGCCCAAACTGATGCGCTTAAGATATTAGCACCTATGGGTAGTGAATACCTGGAAGTGGTAAAGGAAGCCTTTCGGGAGCGCTGAATTTCCTGGTTACCCGATAAGAACAAGTATACCGGGGCTTACTCAATTTCCAACGTTAAGGGTTTAGATCATTACTTCATTTTGATGAATTTTGATAAAACCCGCGCTTCATTAAATACCCTAGTCCATGAATTGGGTCACAGTGTCCATTCTTGGTATGCATCTAAGTACCAAACGCAAAACTTGGATCCTACAATTTTCTATGCTGAAATTGCTTCCATTTGTAACGAATTACTGTTGTGTTACCACGATATTATTAACTATGAAAACCGCAATCCGCAGCAGTTAATCCGTAGTTTAATGGAACAAATTAGCCACTTCTTTGGCGCTACCACGAGACAGTTAATGTTCTCTCAGTTTGAACAAGACACCTTAAAGTTAATTCAGCAAAACCAAAAGCCTGACTTTAAGACCCTAGTGGAAATTTATGGTAAGACCGCCATTAAGTACCAAGCTGCTAATGCTGACGCTATTACGAAAAAGCTCAAGCAAACCAAGTACCAAAAGTCATTGGCTTACATTACCTCCATTCCGCATTTTTACGCTGGTAACTTCTATGTTTACAAGTACGCCATTGGTCAAGTTGTGGGTACCTTAGTTGGTAAAAAACTGAGTGCTGGTGACAGTAATATGCTCGCTGCTTACAAGCGCTTCTTGTCTTCAGGCTCCACCTTACCACCACTAGAAACAATTAAATTGTTAGGGATTGACCTTACCCAACCAGAACCGTGACAAGAAGCCCATGCGGAGTTAAAGCGCTGGATTAAACTAGTACAAACAGCCTTTAAACAGCTGCAACACAAAAAGCGCTAGTGCATTACTTTAACCGCGCCAAAAAGGCAAAGAACAATGAATTTTATACCTTGTTTGAAGACATTGCCGCTGAAGTGGCATGTTATCCCAATGCCTTTAAGGGTAAGGTAGTGTTGTGTAATTGTAATGATGGTTACCAATCGAACTTCTGGCAGTTTTTTCAAAGTCAGTTTCATGCTTTAGGACTCAAAAAACTAGTTGCGATTGCTTTTAATCCACTTGGCAACAGTTATCAACTCAACTTCGATGGAAAGGAAATAAAGGAACTACCTTTAGCTGGTAATGGCAGCTTTGACAGTGCGGAAGCCATTGTTCTTTTAAAGCAAAGTGACATTGTGGTAACTAATCCACCGTTTAGCTTGTTTCAGGACTTTGTTTGTCTTTTAGCAGAGCATGGCAAACAGTTTTTGGTTTTAGGTCACAATGGTGCTGTCGGTTATAATCAGATCTTTAAGCTGTTTAAGGAAGAACAGTTGTGATATGGTCATACTGTTAACAGTTCGATGTTGTTCCAAGTGCAGTCTAACTTTAAACTGTATGACCCCAAAAGCGTTAACTTTGTTAAAAAGTATGGTCAACTGTTTCAAAAAGTACCGGGAATTTCCTGGTTTACAAACCTCAAGAAAAACCAACAACCAGCTTGACTAAAAACAAAATCACGCTATCAGGGCAACGAACACAAATACCCCAAGTTTGACTGGTATGATGCCATTTTTGTCAGTAAGGTTAAAGAAATTCCCCTAGACTGATTTGGTTACATGGGGGTGCCATTAACCTTTCTCAATTGCTTTAATCCCAAACAGTTTGAGCTAATTGATTGTCTAGCCAATCCCTACGCTACCCTAGACACATTAAAAACTAATGCTTATGTGAGATCACACCACGGTGATGTGAGGAATGTGAAGGGTAAGCGAAGGTATGTTAGGGTAGTGATTAAACAACGTCAAAATGTGATTTAATGCCTTAAGTTAAGTAATTTTTGCAAAAAATTTCCTTTAATTTGCTAAATTATTTTAAGGATAGCACACTGCTGTCAAACCAAATGAGAACGAATGAAATTCAAAATATTTTTGGGCAGCAGCTTTTTTGGTGTTGCCACCTTACTTAGTGCTTGTGGTACTGGTCGCTTTGATCAAATCGATGATGGCAAAATTAAGCTTGCTGTTGTCACATCCCCTAGTGCCGCTAGTTCACTGCAAACCTTATTAGATCAATACAACAACACTAAAGCACCAGCTGATTATCCAGTGGAAGTAGTTTCACTAGACAGTACTGGGAGCTATACTAAAGGGAAATTTGATACCCAAAAACGGTTAGCTGCTAAAGATAAAAATAACTTTTATAACCTAACCTTTAACTACCCCGACTTAGTTTCATCTTTAGCAATTAACGGAATGGAACTCAACCTTGATGGGGTTAATGTTTCTAACTTCGAACAGTCTTTCTTAGACTTCAACAAGAACATTAGTGGCGTACGCAAACCCGGCATTTACGCCTTACCAGCTACCATGAGTGGTGAAGTTTTGGTTTTAAATGGGCCAGTTCTCCACTACATCTTGAACAGTGCCAAGAAGAAGGATGGTACCGAATCAAAAATTAAAACTGCTCAATTAAAAACAGCTTCAACTCAGGCAGAAGTTAAGGGTACTATGACCATGGCTAGTGATGAACAAACCACAAAGCTTTGGACTAATATTCAAAACGCCGCTAAGGAAAATGCCGATAACGGTACAACTGAGAAAGCGGAAAAAACAGTTTCCGCTAGTTCGTTGCAATTAAAAAATACGAATAAAACCACCGAAGGTACCCTAAAAATTGGTACTGATGACAACACTAAAAATCTTTGAAAAAAGATTGAAGATGCCGCAAAAACTAACGGGGAAAAAGGTAATGAGAAGCAAGAAGCTACTCAAAGCAATGCTAGCGCTAGTCTCGTTAAGTTGGATCAAAAAATACTAGTCAGGATAAAACTCAAAACACCCAGACTAGTGATGATGAAATTAAAAAATCTTGAGGCGAATATAAGGAAGTAGAAGGCGGTCTTAAGGGTTATGAATTTAAGGCTGATGTCTTTGAGAGTTGGGAAAAGTTAAACGATTTTGCAGTACGGGTAGCAAAGTCGTTTAGCAAGGTTTCTGAAGAGAAGAAAAGTGGTAGTGACATCCAGGGTGTTTTTGGCATTGGTAGTTTAGAAAATGCGCTTTACACTGCATCATTTGCAGCCGGTGGTGGCGACTATAACAACTTCTTGTTTAACATTAAAAAAGGTCGCGCTGATTTTAGTAACTTCTTTAACCACAATTCAAAAACTTTCCAAAGTCTAAGAGATATTTTCAACAGCTTTAAGCCTTTAATTGACCAAAAGGGTTTAATTAATAACAAACACTTTGATACTCCAGTGAATAATTACGCTAAATTTCACCAATTGGCATTCTATGTATCATCGACAGCTCGCTTTCCTTACTCGTTTGCCAAAGATAATGTAAAACGCTTAATTATTGGTAAACGCGAATTGGAAGTAAACCCTAAATCAATGTTTGCCATTAAGAAGGAAAACGGCAATAACGGTAACAGCAACCTCTTAGGTAAAGTTGCACTAGATAACAATAAATCTATTGAACTTTACGAAAATAATATTCCTAACGGTAAAACTGATGCCATCTTAATTAAGAACCAAACTTTAATTAGTGCGTTGAAGAATCCAAAACAGACAAAGAGCTCTCAAAGATCTTCTCAGTCTACAAACACCCAAAATGATGCCATTTGTTACTTAGCCTTCAACAGCAAGATTAGACCAGACGATAAAGACATTTTCCTATTAGACAAATTTGGTGAAAAATTTGTCACCGCAATTGTTAACTTTGAAGAAAAAACGGAGGTCAAAATTAATACGCTCCAAGAAAAGGAAGCTGTTGTTTTACCAGCACCACAAAAGTTTAAACCTACTGATCCAAAATCAGTGGCACTTGTTCAAGGTCCAAGTTTAATCGGTATTCACGCTAACGCTAACGAAGACCGTTCAACCATTAAGTTTCTCAACTGGTACCTTAATGCTGAAGTTGATTGAAAAGATGGAGAAAAGAAAACACCAGCAGAATACTTAGCAGAAAAAGCTTCTTACCTATTGCCGTTTAAAAAAGTATTAGAAAAATCCAAGCAAAACATAAAAGCGACCTCTAAGGAAGGAGAGCAAAATCAAGGCAAGAAAGGTGATGGTGCACAGAACCAAGGTAAGAAGGGCGATGGAGCTCAAAATGGTAAGAATGATAAAGCAAAGCACAATGGTTTTGTCGATATTGCCGTCAGCCAATTTTTAAACGGTTCTGTCGCTAAGTTTGTGGAACCAGCTGACTTTTTAGGTATTCGCTTACGTGATGCCGTTAAGAGTACCTTTAACGCTGCCATTAACAACACCTCAATTAACTTTGACAAGTTCATTCAGTACATTAACGATTCTTTAGGTAGCGACTTTAAGCAAAGACAGTAGTTTAAACTGATTCTTTACCTCTAAATAAAAATACTAAAGCCCATTAAAGCCGAACCACTAATTTTGCCCTAGCTTGTAAAGCAGTTAAATATTTTTTAAGATATCTTCACCTCTTTTTTGCGTTTAGTGATGAAATTTAAGTATGGTGCCATTGTTTTCAGTGGTCTTTTAGGAGTCTCTGCCATTTTAGCTGCTTGTGGTACACGCGGTAAATTTGACCAAATTGATGATGGCAAAATTAAGCTAGCATCTTCGTTAACTTCCAAAGGTGCCGCTAATGCTTTACAAGCAATTGTGAAGAAGTATAACGAGGTTAAAAAGCCTGGTGATTACCCAATTGAAATAACCCAAATTGCGGGTGGTTACGACCAAGCACGCGTTGACTTACAATCACGGGTTGGTGTTAAGGATAAAACCAATTTTTACAACCTCATTTTGAACTATCCCGATGTAGTTTCAGTGCTAGCACGTAACCAAATGGAACTGCCATTTGATGGTGTTGATGTTTCTAAAATTTCACCTAACTTTTTAAATTTTAACGAACGTATTAGCGGAGTTTCTAAAAAGGCTAACTATGCCATTCCGGTCTCTGTTTCGACTGATATTTTAGTGCTCAATGGACCAGTTTTGCACTACATTTTAAACAGTGCTAAAGGTGAAAGTAAGGGCGCACAAAAAGATAACAAGAGTGCTGAAGTTCAAAGAAAAAGTACAGGTCAAAAGACCGTTACCCAACCTTTAACTATTGCAACTGATTCTGCTACAAACGGTCTTTGGAAAAAGATTGAAGATGCTGCAAAAGTCAGCGGTAAAAAGAAAGAAGAGAAAAAGAGTACACGCAGTAAAAGAGCCACAGAAGGCACACAAACCACCAAAGAAAATACGGGTGGTGATGCTGCAACAAGTGATACCAAAATTAAAGAATCTTGAGGTGCTTACCAAGAAGTAGAAGGCGGCTTAAAGGGTTATCAATTTAAAGCCATTGTTTTTGAAAACTGACACGATCTAATTGATTTTTCTACCAGAGTAGCTAAATCATTTAGCAAGGTCAAAGATAACAGCAACAAGAAAGGCAATGAAATTCAAGGTGTGTTAGGTGTTGATAATTCACCTAATGCTTTACTATCTTCGGTGTTTGCTGCTGGTAACAGTGACTATAACAACTTCTTTTATAAGGTGCAAAATGGTCGGGCTGACTTTAGCAACTTTAACAATAAAGGCAGTTCTTATCAAAACCTTAAAAATGTCTTTAACGACTATAAGAACCTAATTGCACAAAATGGTCTTTATGTAAATAAAGGCGGCTCTTACTCTTCAAACTTCCAAAAGTTTCACCAGTTAGCTTACAGCATTTCCTCTACTTCTGGTTTTGCTTATTCGTTTGCTGGACAAAATTCCAAACGTTTTAAATTTACTGATGATGGTACTTTTGTTGAGTATCCTAGTTATACAACTGAAGTTAATGCACCAGAAAGTAATAATGGCAATGATGGAAAGCAACAAGGTCAAAGTGATCAAGGAAACCTTTTAGGAACTTTTGAAGTTGTAGATAAAAGTACTAGTGATATAAAGGTGAAACCTAAAACTCAAGCGGAATCAAAGAAAAGTAGTGATAGTAAACAAACTGCAAACACAGGCAAAGGCAGTAATTCAAAACAACAAACTCCGAAGAAAACTATTTCGCTTTATAAAACCAAAATTCCTCAAGATAAAACAGAAAACGTTGACGCTTTTTTAGTTACTGATTCAGAATTAATTAGCAAACTTGAAAAAGCTAAAAATAAAAAGGAAGAAACTAAAGCTTCAGGTAAATCTGCATCTGCACGTGTGGCAGTTCAAGCAACTCAAAAGAAACAGTCTAACGAAAAGCAGATAGTTGGTTACACTACTACATCCGCATTAAGCGAAGACGGCAAACACATTTTTAAATTGGGTAAACTCAACAGCGAGAACTATGAGCGCAAAATCATTGTTGGTGCTACTGTAGAAACACTAGAACAATCCACTACCTTGCAAAGTGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAGTACAAGGATAGTGATCAAAAGAGAGTTATGATCACCCAAGGTCCTAACCTAATAGGGGTTCATGCTAATACTAAAGAAAATGAAGAGACTAAGAAATTTGTTAACTGGTTTTTAAATAAAACCGAAAGTTGAGAAGTTAAAGGAAACGGAAAGGATTCACAAACTACTAAATCACTTACACCAGCGCAATATTTTGCTGAATCAGCATCTTATATTTTGCCGCTAAAGGAAACTGTTGAAAAAGATCATAAAGAACAAACTAATAAAAACACCTATGTTGCCAAAGCTTTAGAAATGCTCAAGGAAGTTAGTGAAAACAAATCAGTCAGCTATAGTGATCCGAGTGATTTTAGAAGTGGTAGATTCCGTGATGCACTTGGTGCTAACTTTAATGCCACTATTAATTCAAAAGCAAATTTTGAAAAGTTTTTTCCAAGGTTTTAAAGCTGCATTAGGCTCTGATTTTGATAAATAGAATAGAAGTTTTTAGTTGCTTTTTAGATAAAAATTTCAAATATTTCTCCTTTATAAAACAATCTTTCAAAAAGCTTTTTAGAAAGAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATCAAAGATATTTGTGATATTACACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGAAAGTAAAAAATAAAGAAATTAGTTCAAAATATCTTGCTCATATTTTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGACCTAAGTTAACACATAAAACAATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGGGCGCGCAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTTAATCAAGAAAATATCCGAAAAATTTATGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACACATTAAAGATTGCGACTTTGATGGTGAATACATCACTTGAACTACAAATGGTTATGCTGGAGTAGTTTTTTATCGTAATGGCAAGTTTAATGCATCACAAGATTGCGGTGTTTTAAAAGTTAAAAATAAAAAAATCTGCACCAAATTCCTATCCCTTTTATTAAAGATAGAAGCGCCAAAATTCGTTCATAATCTAGCTTCAAGACCAAAATTAAGCCAAAAAGTAATGGCTGAAATCGAACTCTCTTTCCCACCTCTAGAAATCCAAGAAAAGATTGCCGATATTCTCTTTGCTTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTTCAAAAAATAAGAAATTAGAAAGTCTCAAATCTCTTTAATAATAAAAGTTAGTTCAGCAAACCACAAACTGATCCCACTTCTCCCCCACGCTCACCCGCTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTGCTCGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGTACAGGTATGGCAATAACCACCGGGGCGTGGATGATATAACCGCGCCTCAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGTCTCAAAGCCAACGTCCAAGCCACGCTCGGTGGCAGTCAGACGACGACTACAGGCGGCTCCAAATGATCCACCCTCGACAAAGCCAACCTCCAGCCCTGAACGGGGGCGGGGTGAAGGAATGATAAGACTACTGGGAATAACACCCAAGGCAACGAAAACCACACCACCTTTAAAAACGCTACGGGGATGGGCAGTCAAAACCAGGGTTCTACAACAACTACCTCAGCGGGCAATCCCGATTCTTTAGTTACTGATAAAGTTGATCAAAAAGGTCAGGTGCAAACAAGTGGTCAAAATTTAAGTGAGACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACATCCGACTGACCGAACGCGCTCAGCTTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCACGGCTTGTTGGGCAGCATCCCGGTGTTGGTTAATAAGAGTGGCGAAAATAACGAAAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGCTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTTGGTAACCTCGTTGTCAGTGGCACCAGCTTCAGCTTCCAGCTCGGCGGGTGGCTGGTCAGCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGACTCCAGTTAACGGGCTTGGATGAAAGTGATGCGACGCAGCGCGCCCTCATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGTTGGGCCGCGTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGACCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTACCCAATCCGACAAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGCGCCAGAATGCTCAAATCATTTGGTACAGAAAATTTCACCCAGCCCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGGTACCACGCAGGGCTCAAGCACCACCAATCAACTGTCACCCGTTCAACGGGTGAGTGGGTGGACCGCTAACTATTAATAGGAACTTTTCTTATTAAGAAGAACTTTTTTGAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTTAACGAATACCCAAAAAGTATGAAATTGATTAAATTAGCGAGGTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTTCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTGTTGACACCTTTAAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAAAAGGAGGTATTACTTAATGAAAGAAAAAACTCCCTTTAAAAACGAAAAAGAATTCCATGAAATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATATATCATTGACAAAGATAACAACAGTGTGGAATTCTCTGGCAAATTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAGTTAAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAGCAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGCTTGGACAAAATTGATCCATCTAAATAGTTTTTAGGCTAATAACAAAAGTGCCAGCAGTTAATCTTAAAGATTAGTTGCTGGTTTTTTTCTTCACAACCAATAAACAAATAAAAAGCCATCCACCGCGAGGTGAACGGTAGATGGCTAAAAATAGTTTTTAATGATTTTGAAAACTAACGAAAAGTGTTAGTTTGAGTTGTAAAGCAGTTATGAATGTTTGTTCCTTTAAAAATTTGTTTTTTGTTTAGCTAAATATGTGCTGAAAGCCATTGCGTTAACATAAGTAAATTAAGACATAAAAAATGAACACATTAATCAAATAAGTGTGGGAACAGAAAATCAGGCTTTCAACACAAAATTTTCGTTTTTCTGCTCTCACTAAATAAATTGAGTTGGGTTTTAAAAACCGCCAAAAATTTTTAATTTAGTTTTTGCTACTTTCTCCTCCCTCCCCCTCACGGGTGAAAACCCCGGGGCGTGGGCCTTAGTGCGCGATAACACTGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCTGCGCGAACCGAGGCCACCTTGACCACCATCACCTTTGCGCTGCGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCAACGCGTGATGCCAACGGCCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGCAGTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCCCAAGACCCCACCGATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGTGTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGTTCGACCAATTCCGACACCAAAGTCAAGGCTTTAAAAATAGAGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGCTGCAGTTAGAAAAAAATGATCTCGCCAACGCTCCCATTAAGCGGGGTGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGAAGGCCGTGGCTTGCGACTGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAACCGTACCGCCATTGACCGCGTTGATCACTTGGATCCCAAGGCCATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGTTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGACCAGGGCCAAGCGGTCGCGGATAATACCCAAACCGGGTTTGATGAAAGTAATACTTCGAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCCATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGTGGCAATGGCCATGTTACCAAGTCGGCCCACACCGCGCCTTTGAGTATTTGGCTTTATATATATTTAGTAAAGGCAGTTACTTTTAGGTTGCTTTTAGCTAACTCTTTATTATCAAAAAGCAACATATATAAAAAACAGCTAATTAAAGCTAGTAGAGCTAAGCGATTATTTCTTTTAAGTTAAATCTTAGTTGCTCAAGATTAAAACGCACTAAATTGAATGCTGTTGTAATAAGGCGTTTTTTCAAATCAAATTCATACACTAAAAGTGCGAAAAGGTTGTATACGCATGGAAAATGCGCGAGTTCTCTTAATTGTTTTCGGTATCGTACTAATTTTTGTTGGTATTGGCTTTTTATTTTGAGCATTAAGAATTACGCTTAAGCGTCAAAAAGGAGGCGAAGCTACTACTAGCTTAACGACTAACGATCCTGCTTTAAAGAACTTTGGTCGGCCAAGTTGATGGCAACGTAATAATCAACTTGTGCTCTTCCTCTTAGCGACAGTATTTTTGATGTTGGGAATAGGCGGTCTTTCTTCCATTCCCTCAATGTTTAAGTAACAAAAATTTAAGGATTTAAAAAAGGTTCATTTATGCAAATTAAAGCACAGGACACAGGACAGCAAAAGAAGTCCTGTTTATTGTCTAACATCCGCAATAAATGGAAAAACCGGAATCGCGGGAGTTTCCGCCAATGAGTTGGAAAGCTTTCCAATGGGTTGATGATCCCGATTGCCGTTTTACCTATTGCGGGTATTTTTTTGGGTGTTGGTGATGCAATTGCCGGAAATGCAGGTGATTTAACTGGTCTGCGTTATTTCGGACTTTTCATTAAAAATGGTGGTGATGTTGTTTTTGCTAACCTACCCATTTTGTTTGCTATTGCTATAGCAATAACGTTCTCACAAGACGCTGGGGTCGCCGGATTTTCGGCCTTTGTCTTTTGAGCTGCTATGAACGGGTTTATGAGCTCGTTAATATTGCCTTTTGATAAAGCAGGCAAAATTATTACTGACACTTCGACTCCAATTGCTGGTTTTAAAGTACTTTACTACAAGAGTGTCCCTGTTCATGCTATTGCTACAACCTTGGGACTGCGAACATTATCAACCTCAGTATTCGGTGGGATTATTGTAGGTGCCTTAACTTCAGTACTTTACAAGAAGTTTTATGCCATTAGGTTACCTGATGTAATTGGATTCTTTAGCGGTACTCGTTTTGTCCCTATTATTTGTTTTGTGGTAGCGATTCCTGTTGCGTTAATCTTGTTAATGATCTGACCAGCTGTCTCCATTGGACTTAATGCTATTGGTACTGGTTTAGGTTTCTTAGGTGGTAAAGGTTATGGTGCCAACAGTTTGATCTTTGGTTACATTGAACGTTCCTTAATTCCGTTTGGTGTGCACCACGCCTTCTATGCACCACTTTGATACACCTCTGCTGGTGGTAGCTTACAAGAAATTGTTAACCAACAGGTTTGGATTCGTCCCGACTTCCACTTAAGTGATAACTATGTAGCACGGGTAATTGGTTGAGTTGATCCTAATAACTCTAGCATGTACATTATTCCTGGTGCATTAAATGGTCAAAATGGAAGTTCAACCGGTAATACAATGTCAAAAGATCTTAATGGAGCACTTTCTGCCTACATGAGTAAAGAAAGCACAGCTTTCTTAACTTGAAAAGATCTAGTTGATGGCTTAACCTTTAAAGGTAATTTTGATAAGATGGCCGAAAACGGTTTGTTAGACGGTTCTAACAAAATTTGATTGGGTCTGAACGGTTCTGGTATTTTAGGTAAAAAATTGTTACTAAGTGATGGTAATGTTTACACTATTACCTTTAAAACCTTTGCCAACACTACTCCAATTGCTTGGAGTAAAGGTGCACAGGCAGTGTTACCTTTAAATGCTAGCTCTACAATCGTTAACAATCCAACCGCTTTAGCAGCAGCTACACAAAGTAATAATAACACTAACAACATTAAACTTTACCCGGTAAATTCCTTTAGGGTAGCTGTTGAAAGTTTAAATCCAGCGCAATACTCCCAGGGTAAATTCCCCTTCATGTTGTTTGGAATTCCAGCAGCTGGTGTAGCCATGATCTTGGCAGCACCTAAAGATAGAAGAAAGGAAGCAGCTTCGATTGTTGGTAGTGCAGCATTTACCAGCTTCTTAACTGGTATTACTGAACCATTTGAATTTACCTTCTTGTTCTTAGCGCCTTGATTATTCTATGGGGTGCACGCTGTTTTGGCAGCTGTAAGCTTCTGGTTAATGAACATCTTGGGTGCTAATGTGGGTCAAACCTTCTCTGGTTCCTTTATTGACTTTATTCTCTATGGTGCTTTACCAGATGGTCGTCGTTGGTTAGCAAACTCTTACTTAGTACCTATTATTGGACTGTTCTTAGCTGCCATCTACTTCCCAACCTTCTACTTCTTAATTAAGCACTTTAACTTGGCAACTCCAGGTCGTGGTGGTAAGTTAATTACCAAGAAGGAATACTTAGCAAGCAAAGCAGCCGCTAAAGCAGAGGGTGTAAGTGGTGTAGCAGAAAACTTTACCCAAACGCAAATTGAAGCGGGCATCTTACTCCAAGCTTACGGTGGCAAGGAAAACATTGTTGAACTAGGTGCATGTATTACCAAGTTACGGGTTACTGTTAAGAACCCCGAACTAGTTAAGGAAGAACCTATTAAAGAACTTGGAGCTGCTGGGGTAATGCGTACCACCCCAACCTTCTTTGTAGCCGTTTTTGGTACCCGCGCTGCCGTTTACAAATCTGCTATGCAGGACATTATCCAGGGCAAGGTTAACTGGGAAGCGTTGCAAAAGGTTATTAATACAGATCAACTAGCTGTTGAGCCTAAAGAAACCACTCCACCAAAGGAAGTAATGCCGGTGGTACAAGACGAAATTGTGATTCTGTCCCCAGTTAACGGTACCTTAAAATCACTCAATCAAGTACCCGATGAAACCTTTAAGCAAAAGCTAGTGGGTGAAGGTGTTGCCATTGTACCGAGCGATGGTCACTTTAAAGCCCCTGGTGAAGCAGGTGTGAAAACGGAGTTAGCTTTCCCTGGTGGTCACGCTTACATCTTTGACATTGATGGCATTAAGGTAATGTTGCACATTGGGATTGACACTGTGCAAATTAACGCCAAAAAACAACCGGGCGAACCACTAGAAGTGTTTGACATTAAGACCAAACAGGGTGAGTACACTAAGGAAAAGAGTGAAAGTGTAGTGGAAGTTGATTTAAAGAAACTCAGCAAGAAGTACAACCCAATTACCCCATTTGTGGTAATGAAGGAATCACTAGAAAACTTTAAATTAGTGCCAATCCGCCAACGCGGTGAGATTAAAGTGGGCCAACCAATCTTTAAACTAGTCTACAAGAAGTCACAGGCATAAAAACTGCTTGGCAAACAAAGAGGTCATAGGTAAGCCTATGGCCTTTTTGTTTATAATGAAAGTCCGGGGAAGGCTTTAGATGAATTTTTCCCTGGTGTTTCATCTGGTTATCTTTTATAAGTTTTAGCACCTGCAATTTTTTATGTTCTTGCACGCGTGTAGCAAACATCTTTTAAGTTTTTTCATTCTTCAACTTTAATTTCTTCAACAGTTTTCTAGTAGTTGCTTATTTTTCAAACTTAAGTTAGTTAGTTAAGTATTGCCTCTTTTTTTGAAGCAGCTACACTTATCAAGTTACTCATATTTTTCTTTTTCATATTTAGACATAGGTTGTGTTAAGGGATTTTTAGGACTATCCATGTAAATATATCTGATAACTTATTTTGATTTTGTTAGCAATAACAACAAAATCCACATCTAAAGCAAACCAAAAAGTCACCTGTTTTTAACGGGTGGCTTTTTAATTGTGGAACTGCAAGCTCCTTAATAATTCTTATTAAGGAGGATGACAATGTTTGGCTTAACACTTTGGCACAAAAATGGTGTTGGTTAGCAATCATTAATTAAGTTAACTTGCTCTGTTTGGTGCATAATGTAAAATTACAAAGCATGTCAGAACTGATTACAACACCTGTAGAAACCACCGCCAAAGCGGAACTCGTTTCCCTAGCTAAGCTCGGGGAAATGCGTACCCATGTCGGAATGGTTAAGCGTTACTGGAACCCCAAAATGGGCTTTTTTATTGAACCAGAACGCAAGCACAATAACGATCACTTTGTATTAGAGTTACAACGTCAATCTTTGCAAACAGCTTACAACTACGTCAAGGAAGTAGCACAAAACAACGGTCAAATTCTCTTCGTTGGTACTAAGAACGATTATGTGAAAAAACTCGTTAATAATATCGCTAAACGGGTTCATGTTGCTTTTATTACTCAGCGCTGATTGGGTGGTACTTTAACTAACTTTAAAACACTTTCAATTTCAATTAATAAACTCAATAAGTTAGTGGAAAAACAAGCGGAAAACGCGGCTGATTTAACTAAAAAGGAAAACTTGATGCTTTCCCGCGAAATTGAACGCTTGGAGAAGTTCTTTGGCGGGGTAAAATCACTTAAACGTTTACCGAACTTACTAATTGTGGATGACCCGGTATACGAAAAGAATGCGGTAGCGGAGGCTAACAGCTTGAGAATTCCTGTAGTAGCGTTGTGTAACACTAACACCAATCCGGAATTAGTTGACTTTATTATTCCCGCTAACAACCACCAACCCCAATCAACCTGCTTGTTGATGAACTTATTAGCTGATGCGGTGGCTGAAGCTAAGGCAATGCCAACCATGTTTGCTTACAAACCGGATGAGGAAATTCAAATTGAAATTCCCCAAAAACAAGAGGCACCACGCCAAGTTGTAAACCGTGCAAATTCTAAACAAATTACCTCTCAACGGTTAAACATTACTCGCAACCCAGAAGTTCTCACCCGTGAATAAGTGAACTGGGTTGAGTGCTGCGGCCGTTTTAGAAAGCCGCGCACAGCATGGTGCTAATTTAATTCCTACAAAAAAATTAACCCCTTTTTGACTACTGTTCTTAGAACAGTTCAAGAGCTTAGTGGTGATCTTACTGCTGGTAGCTACCATCCTTTCACTAGTGGTTGCCATTATTAGTGGGGTTAATGCTAACTGGTTGTTTGACCACAACCTTGTTATTGAGTGAACCCAACCGTTTGTAATTTTGATAACGGTGTTGGCCAACTCCCTGATTGGCTCCATTCAGGAATTTAAAGCGCAAAAGTCAGCTCACACCTTAAAGTCCCTAACGCAACCCTTTACGCGGGTATTCCGTGAAGAAGGCTTGGTTTCGTTACCGGTGGGTGAAGTTGTTGTCGGTGACATTATCTTTCTGGAAGCGGGTGATATTATTCCCGCTGACGGGAAGGTGCTGCAAGCGAACCACCTCCGCTGTATGGAGAGCTTTTTAACTGGTGAATCAGTCCCAGTTGATAAAAGTGTGGTTAACACCGGTGGCAAGGGTTTGTTGGAACAAACAAACCTACTCTTTTCTGGTGCCCAAGTGGTCTTTGGTAGTGGTGTATTCGAAGTAACTGCTGTGGGGTTAAATACCCAAGTCGGTCAAATTGTCAAAACAGTGGACAGTAGTGCAACTAAGCTATCGCCCCTACAGCAAAAACTGGAAAAAGTGGGGAAGTGGTTTAGCTGGTTTGGCCTTGGTTTATTCGTTGTCGTTTTCCTAGTGCAGTTAGGGTTACTTGGTTTTCACAACTTTAGCGCTAACTGATCGATTGCCTTAATTGGTGCAATTGCCTTAGTAGTCGCTATTATCCCTGAGGGCTTAGTGACCTTTATTAACGTCATTTTTGCCTTAAGTGTGCAGAAGCTCACCAAACAAAAGGCAATTATTAAGTACCTAGCTGCAATCGAAACACTCGGTGGCGTGCAAATCATTTGTACCGACAAAACTGGTACTTTAACACAAAACAAGATGAAGGTCGTGGACTATTTCTGTTTTAGTAACACTACCCAAACAGACTTAGCACGTGCTTTGTGTTTGTGCAACAATGCTACAGTCAACACTAACGGAGACAGCACCGGTGATCCAACTGAAATTGCCCTTTTGCAATGGTTGGACCGTGATGGTTTGGAATTAAACCACTACACACGCGTGTACGAAAAGGCATTTGATTCCAACCGCAAGCTTATGAGTGTGGTGGTGCAAAAGGACAACCGTTTTATTGTGATTGTCAAGGGCGCACACGATGTCTTGTTACCGTTATGTAAGGGCTTGGATAGCAACCAAATTAAGCCTTTAATTGATGAACGTGCTAGCAACGGCTTGCGCAACTTAGCGGTGGGACTCAAGGTTTTATACTGCTTTGATCCAGAAAATACCCAAACTGTCAACGAATTGGAAAGCGAACTCGACTTTTTGGGTTTTGTTAGCTTACAAGATCCACCACGAATTGAAAGTAAAGCTGCCATTATGGCGTGTCACCAAGCCAACATTACCCCAATTATGATCACTGGTGATCACCTCAAAACCGCTACTGCAATTGCCAAAGAATTGGGCATTCTGACCGATGAAAGGCAAGCCATTTTAGGAGTCGATTTAGACCCCGCTAAAATTATGGAGTACCGCGTCTTTGCCAGAGTAACGCCGCAGCAAAAGCTAGAAATAGTTAATGCCTGAAAGCAAGCGGGTTACACGGTAGCCGTTACTGGTGATGGGGTCAATGATGCGCCCGCTTTAGTGACTAGTGATGTTGGTTGTTGTATGGGACAAACGGGGGTTGACATTGCCAAAGATGCCGCTGATGTCATTATTAGCGATGACAACTTTGCCACAATTGTTAACGGGATTGAACAGGGTCGTAAAACCTTTTTGACCTGTAAACGGGTGTTGTTTAACCTCTTTTTAACCTCAATTGCTGGCACGATTGTCGTGTTATTGGGTTTATTTGTATTAGGGGAAGTCTTTCGTGAACAACTCAGCAAAGCAAACCATAACTTCCAGGTCTTTACCCCTACGCAACTGCTAATTATTAACCTCTTTGTTCACGGCTTTCCCGCTGTGGCCTTAGCGATCCAACCCGTCCAGGAAAAACTGATGTTAAAACCGTTTTCCACCAAAAATCTGTTTTACAACCGTGGCGGGTTCGATTTAATCTGACAATCCTTGCTGTTGAGCTTTTTAACCCTACTTTTCTACAGTTTAGGGATGGTCTATGCCATTAATGATCCGGAGTTAGGCAAATCCGGTGATTTAATTAACCGGGCTGGTGCGACCTGTGGTTTTATGGTGTTAGGGGGCAGTGCAGCTTTAAATTCCTTAAACCTAATGGTGGATCGACCATTGGTAGCTACTAACCCAAAGCACTATGGCATTGTGTGACTAGGGGCGTTGTCCTCGATCTTTGTCTTCTTATTAATTATTTTCATTAACCCCTTGGGGTTAGTGTTTAGTACATTAAAAGACCTTACTGCCCACCCGGTTTTAATTGGTTATAGCTTTGGGGGCGTGCTTTTATATATGACAATTAACGAAGTTGTTAAACTTATTAGGTTAAGTTATGGGTCTGTTTAACTTCTTGAAGTTAGTCTCCCCGCGTCATCGGATTTACCATAAGGCAAGTAAGATTGCCAACGAAGTCGAGGGACACAAAAATTACTACCGCAACCTCACTGATGTACAGCTGCTGGAAGAGTCCAATAAGCTAGTGGATTTAGTGACAAAGCAAAACTACACCATTTTGGATGTGGCGGTGGCTGCTTTGGCCTTAATTCGCGAAGTGGTCTACCGTGAAACCGGGGAGTTTGCTTACAGAGTGCAAATTATTGGTGCTTACATCGTCTTGATCGGTGACTTTGCTGAAATGATGACTGGGGAAGGGAAAACCCTGACCATTGTTTTAGCAGCCTATGTCTCGGCGTTAGAAAAACGCGGTGTCCATGTGGTTACGGTCAATGAATACCTAGCGCAGCGGGATGCTACTAACGCCACCAAGATCTTAAAGCGTGTTGGCATGACCGTTGGTTGTAACTTTGCTAACCTAGCACCACATTTAAAGCAAGCCGCGTTTGCTTGTGATGTAACTTACACCACTAACAGTGAACTCGGTTTTGATTATCTCAGGGACAATATGGTGCACCGCTTTGAGGACAAGAAGATCCGCGAGTTGCACTTTGCGATAGTAGACGAAGGCGATTCGGTTCTCATTGATGAAGCGCGCACCCCTTTAATTATTTCTGGTCCTGCCAAAAACGAGTTTGCAGCATACGTGGCTGTTGATCGCTTTGTCAAAAAGCTCAAGGAAGACGAGTACAAGATTGACCCCGAATCACGTGCCCCCGCTTTAACAGAACTTGGCATTAAGCACGCGGAAAAGAACTTTAAAACCGATAACCTGTTTGCGCTAGAAAACAGTGACCTGTTCCACAAGATTATTAACGCTTTAACCGCCGTTAAAGTTTTTGAGCAGGGCAAGGAGTACATTGTGCGTGATGGTAAGGTGTTAATTGTGGACCACTTTACTGGCCGAATCCTGGAGGGCAGGAGCTACAGTAATGGGCTCCACCAAGCGGTTCAGGCCAAGGAAATGGTCGAAATTGAACCAGAAAATGTGATAGTAGCTACGATTACCTACCAATCGTTTTTCCGTCTGTACAACCGGTTGTCAGCGGTATCGGGTACCGCTTTTACTGAATCGGAGGAATTCCTCAAGATTTACAACATGGTGGTAGTACCCGTACCTACTAACCGACCCAATATCCGCAAAGACCGTGCTGACAGCGTCTTTGGTACGCCCAACATTAAGTGGCTAGCAGTGGTCAAGGAAGTCAAACGGATCCACGAAACCGGGCGACCAATCCTAATTGGGACCGCCAACATTGATGACTCGGAACTACTTCACAACTACCTCCAAGAAGCCAACATTCCCCACGAAGTTCTCAACGCCAAGAACCACTCTCGGGAAGCGGAAATAGTCGCTAAGGCGGGCCAAAAAGGGGCGGTGACCATTTCTACCAATATGGCGGGAAGAGGTACTGACATTCGTTTAGGTGAAGGGGTCGCTGAAATGGGCGGGTTGTATGTCCTTGGTACGGAACGCAACGAATCGCGCCGGATTGACAACCAACTGCGCGGTCGGGCGGGTCGGCAAGGCGATCGCGGTGAGACCAAGTTCTTTATCTCCTTGGGTGATGCCCTGTTCAAACGCTTTGCCCATGACCGGATTGAACGCGCCATTACTAAGTTAGGGAACGATACCTTTGACAGCTCCTTCTTTTCCAAGATGTTAAGCCGTACCCAAAAACGGGTCGAAGCAATTAACTTTGACACCAGAAAGAACTTAATTGATTATGACCATGTGTTGGCCAGTCAACGGGAATTGATCTACAAACAACGCGATAAGTTCTTATTAGCCACTGATCTCAGTGACATGATCGACAAGATGTTGGAAAAGTTTGTCGAGCAGTTCTGTGACCAGTACCGCAACCCTAAGAACCAAAACCTCGTTAACCACATTGCTTTATCAGAGGCCTTAAACCTGGAATTAAACATGCACGGTGTTATTAGTCCGAAGCTGTTTGAAAACATGACTTTTGATGCGACTGTCCATAAAACCCACAGTTTAATTGGTGAAAAGATTACCAATAAGGTCAAGGTCTTAACCCCCCCAATTGCCCTCATCCGTTTTCGTGAAATTATGATCACAGCAATGGACAAGCACTGAATCGAACACTTAGACAATGTCTTTAAGCTCAGGGAAGGGGTTACCTTGCGCTCGATGGAACAAACGAGTCCCCTCAACGTTTACATTCGCGAAACCGACATCTTGTTCCAAACAATGTTGCAAAAGATTGCTCGGGACGTCATTATCCAAATTGCTAACTTAGCGACTCCCGAAGAGTTTGATGAGGAACTAATGAAGGCTAACGCCTTGAAGAAGTTGCAAGCACTTAGAGAAGCTCATGAAAAGTCCAACCAAGGACAGTAAACTGTTCCACCTCAAGAGTAACTTTGCCCCCACTGGGGACCAACCAGCGGCGATTGCTAAGTTAGCTGAGTTTCAAACCAATGAACAGGTCTTACTCGGTGCTACGGGTACCGGTAAGACCTTTACGATTGCCAACGTTATTCAAAAGGTACAACTGCCTACAGTAGTGATTGCCCACAATAAAACGTTAGCGGGACAGCTTTATCAGGAGTTGAAGGAGTTATTCCCTAATAATGCGGTGGAGTACTTCATCTCCTACTTTGACTTTTACCAACCAGAGGCTTATCTGCCCGCTAAGGGGGTTTACATTGAAAAGAGTGCAACTGTTAACGAAGAGATTAAACGGTTACGAGTGTCCACCTTACATTCACTCTCTACCCGCAAGGATGTGATTGTGGTGGGTTCGGTAGCCAGTATTTACCCAACTTCTTCTCCCGCTGATTTCGCCCAGTACTCGCTCTGGTTAGTAGTTGGTAAGGAATACGGTTTAAGTGAGTTAAAAACGCAGTTAATTCACCTAAACTATGTTGTTAATAAACAACAGTTAACTCCGGGAAAGTTCCGCTTTCAGGGCGATGTTGTTGAAGTGTTTCCTGGTTATGCGCAGGATTATGTCTTGCGGCTTTCCTTCTTTGACCAGCAACTAGAACAAATTGCTCGCATCGATCCCTTAACTAACAAGGTATTGGAAACCCTTAACAGCTTTAAGTTAGGTCCCGCTGATGAGTACATAGTAAACCAAAATGACTTAGGAGTAGCCTTAGACACGATTAAGGCCGAACTCAAAGATCGCTTAAAGTACTTTGAACGGTTAAACTTCCCCGAACGTGCCCAGCGCTTACAAACGATTACCGAACACGATTTAGCAGATCTGAAGGCCTGGGGGGTATGTAGTGGCGTGGAAAACTATGCGCGGCATTTAGAACACCGCCCACCGCACTCCAAGCCGTATAACATCTTTGATTACTTTACTAAAGGTGAATGGCTATTGGTAGTTGATGAATCCCACCAAACGCTACCGCAAATTAAGGGGATGTACAACACCGATATTTCACGGAAACAAAGCCTAATTGAGTACGGTTTTCGCCTCCCTTCAGCTTTAGACAACCGTCCTTTATCCTATGAGGAATTCCGCCAGGGAATCAACAAGGTCATTTATGTATCCGCTACCCCTAGGGAAGAGGAAATTCAGTTAAGTCACAACAACGTGGTCGAACAGTTAGTGCGTCCCACCTACCTCTTGGACCCGGAAGTGATTGTGAAACCGAAGGACAACCAGGTTGAAGACCTAGTGAGTGAAATCATTAACCAAAGAAAACACAACGGTCGTACCTTTGTCACGGTGTTAACGATTAAGATGGCGGAAAACCTGACTGACTTTTTGAAGGAGCGCAACATTAAGGTAGCGTACATCCACAAGGATATTAAAGCCTTGGAACGTTTAATTCTGTTAACGGATTTACGCAAAGGTGAGTATGAGTGTTTAGTGGGGATTAACTTACTGCGAGAAGGTTTAGATGTTCCTGAAGTTTCACTCGTAGCCATCTTTGACGCTGACATTCCTGGATTACCACGCGATGAGCGCAGTTTAATCCAGATAATTGGCCGCGCTGCGCGAAACGTTCATGGCCGGGTGATTATGTATGCCAATACCATTAGCGAACAAATGGATAAGGCGATTAAGGAAACGCAACGCCGGCGCACCATCCAAATGGCTTACAACGAACAACACCACAAAACACCAATGACGGTGCAAAAACCAATTACCTTAAACCAGCCGATTAAGTTAAAGACCAAAAGCTCAGAGCAGCAAAAAGCTGCTTTAATTAAGCAGCTAACCAAGGAAATGAAGCAAGCAGCCGCTAACCAAAACTATGAGTTAGCGATTGAGATCCGCGACTCGATCTTTGAATTGGAAAAGCAATTTCGTGGTAAAATTAAGAGCTAGCAATGCGTAAACTAATTAAATTAAACGTCATTGTCTTTGTCTTGTTGTACTTGGGCGAGCTGTTTGCCAGCCTTTCGTTCAAGTTAATCAGTTGCCTCAAGACACGCAACCAGTACTCCTTAAACGGGTACTATGCGTTGTTTGTCTTTGTCAACATCATCCAAAAGATGGCTAACTCTTTCCAAAAGTTAGCTTCCTCAGTTGTGTTGTTTGAAACTGAAATTAACGAATTTTTAGTTCTCTTTACTGATACAAAGAATAAGCGTGAGGAGAGTGAACCAGTGCGCCAGGTGTCAACAACCCAAGAGTATCACCAGGTTACGCTCGACCAACAACACTACTTTAACCACAAACTGAGCGATTACTTCCGTTTGTTTAAGGACAAAACTTTCTTCTTTGAAATTATCTAGTTACTAAATTGACCTGAATTGCAACCTTTCAGGCACTTTTTACTGTTAGTAACTAGTCTTTTTTCATTTCAATATTTAAGTTTTTAATTTAACCAATTTTTACTATGAAGCTTAGTGCTATTATCTCCCTATCAGTCGCTGGTACTGTGGGAACAACTGCGGTGGTAGTACCTACAACTATAACGCTTGTAAATAAGACCCACCAAGTAGAACATGAATCAGAACAATCGGATTTTCAAGATATTCGCTTTGGTCTTAATAGTGTTAAGTTGCCAAAAGCACAGCCAGCTGCGGCAACTAGAATTACCGTGGAAAACGGGACTGATAAATTAGTCAACTATAAGTCCTCACCACAACAACTCTTTTTAGCGAAGAACGCGCTTAAGGATAAACTCCAAGGTGAGTTTGATAAATTCCTAAGTGATGCGAAGGCCTTCCCAGCGCTAACCGCTGATTTACAGGAATGGGTTGACCAACAGCTGTTTAATCCAAACCAAAGTTTCTTTGATTTAAGTGCGCCCAGGTCAAACTTTACCCTTTCATCTGACAAAAAGGCTAGTTTAGACTTTATCTTCCGCTTTACTAACTTCACCGAATCCGTTCAGTTGTTAAAACTACCAGAAGGTGTATCGGTTGTAGTTGACTCCAAACAAAGCTTTGATTACTATGTCAATGCTAGTGCCCAAAAATTATTAGTTCTACCGCTGTCTTTACCAGATTACACTTTGGGTTTAAACTATATGTTTGACCACATTACTTTAAACGGTAAGGTTGTCAATAAATTTAGTTTTAATCCGTTCAAAACGAATTTAAACCTCGCCTTTAGCAACGTTTACAATGGCGTTGATGTGTTTGAAGCACAAAAGAATTTAGTAGGTAAGGGTAAATACCTCAACACCCACGTGAAGGCTGAAGACGTAAAGAAGGACGTTAATGCCAACATTAAAAACCAATTTGACATTGCCAAAATTATCGCTGAGCTAATGGGTAAAGCCCTTAAAGAATTTGGCAATCAACAAGAAGGTCAACCATTATCCTTCCTAAAGGTAATGGATAAAGTTAAAGAAGATTTTGAAAAACTGTTTAACTTAGTCCGTCCTGGATTGGGTAAATTTGTTAAGGACTTAATCCAAAGTAGTAGTCAAGCAGAAAACAAGATAACTGTCTACAAGTTAATCTTTGACAACAAAAAGACCATCTTAAACCTACTTAAAGAGCTTTCCATTCCGGAATTAAACTCTTCTTTAGGTTTAGTGGACGTCTTGTTTGATGTCATTACTGACTCTGATGGTCTCTATGAAAGGTTGCAATCTTTCAAAGACTTAATCGTTCCAGCAGTTAAAACGAATGAAAAAACCGCGGCTTTAAGTCCATTAATTGAAGAGTTATTAACCCAAAAGGATACCTATGTGTTTGACTTAATTCAAAAACACAAGGGTATCTTGACTAACTTGTTAAAGAACTTCTTAGCTGATTTCCAAAAATCAACGCCGTTTATGGCTGATCAAGTAGCCATCTTCACTGAGTTATTTGACAACGAAGGTGCGTTTGATTTATTTGGTGAGGCTGACTTTGTTGACAAGATTGCCGAACTCTTCTTAACAAAGCGTACTGTTAAAAATGGTGAAAAAATTGAAACTAAAGATTCCCTACTGGTAACATCATTAAAGAGTCTTTTAGGGGAAAAGGTAGCTGCCTTAGATGATTTGTTAGATAGCTACATCTTTAAAAATGAATTACTTAACCGCAGTGTAGAAGTGGCTAAGGCTGAAGCTAAGGACACTAAAGGTGCTACCGATTACAAAAAGGAACAAGCTAAGGCGCTCAAAAAACTCTTTAAACACATTGGTGAAAACACTTTAAGTAAAACCAATCTCGATAAAATCACCTTAAAAGAAGTTAAAAACACCGAAAATGTTGAATTAGAAGAAACTGAAACAACCTTAAAGGTTAAAAAACTCGATGTTGAATACAAGGTAGAACTTGGCAACTTTGAAATCAAGAATGGCTTGATTAAGGCAATGCTCGAATTCTTGCCAGACCCTAAAGATTTAGAAACAACTTTAGATAAACTCTTGTTCAAAGGGGAAAGCTACAAAGCGATGAAAGACAAGTACATCAAGGAAGGTTTCCCTGGTTATGGTTGGGCTAAAGGAGTAGTCCCTGGGGCCTTTGAATCTATTGAAAATACTTTTAAGAGTGCTATTGATAAAACCAAGTCCATTCGTGATCTCTTTGGCGACATGCTCTTTGGTAACGATTTAAGCAGCGTTAAAGAAACTGATTCATTCATCACCCTAGGTGGTTCGTTCGACATTAAGTATGGCGGTGAAAATCTCAATGTGTTGCCAGCTTACTACTCTTTAATTAACAGCGAAATTGGCTATCAAATTATTGGTGTAGATACCACAATTGATGTAACTAAGGTAAAAGTTGAATTAAAAAACAAAGAGTATAAGGGTAAATCGCCAGCTATTAACGGTCAGGTGAAGTTGTCACAATCATTCTTTAATGTTTGGACAAATATGTTTGACAGCATTACCAAACAAATCTTCCAAAAGAAATACGAGTTCAAAGATAACATCCAAGTGTTTGCGCGAAACGAAGATAACACATCACGTTTAGAACTTGACATTTCTGATCCTGAACAACGGGTAATTCCATTTGCTTTTGTTGATGGCTTTGGCATTCAACTCAAAGCAGTTGACAAAAACATTACGAAAGAAGCAGGTAATACTGAGCCAAAATCTCCTGTAATTCAACTTTATGAGGCACTTAATAAAGAAAAAGATCAAAAACAACAAAGTAAACAATCTCCAAAACAACTTGATACTAAAACACAGTTGGGTTACCTATTGAAATTAGGCGACAATTGGAGTAAAGATGATTACAAAAGCTTAATTGATGATACGATCATCAATAACAACTATTTAGAGGCCAGCTTTAATTCCAAGATAACGGTTGATCGCTTGGGTATTCCTATTGACCTTTGATTGTTTAAGATTTGACCTAAGTTCAATTTGGAAATCCCAATGCAAGGTTCCTTACAACTTTACAGTAGTAGTGTTATCTTCCCATACGGTATTTATGACACCAGTGTTCAAGATGCTACGAAGATTGTGAAGCGTCTGAACTTTACTGACATGGGCTTCAAACTCAACGATCCAAAACCTAACTTCTGGTTCGTTGGTTTTTAGAGCAGACTAACTACTATACTGATTAAGCTACCTAGAAGGTAAAGCGAGAAGTTCATCCACCAAAACCCGCGGGTTTGTTTACGGTAGGATGAACTTTTTGCTTTATACTCGTCAAAACTAATGTTGGGCTTGGCGTATATACTACCTAGTTTTAGATATTGGTTAAACTTGCTAATTTCGTACCGTGCTCCATCACAGGTCTTTAAGCGCACTATACCTTGGAGACACACAAGCAATCAAACAACGACAAAACTAACAACACAACTGTTTTTCAGAATTAGGTTAATGTTGTTAGTTTTATTGAAAAAAACGGTTAAATTCAAAATGGCGAAGAAAAGCACAATGTAAATTGTGCTTACTATTAACCCGCCGTATTCACGCGGCTTCGAATTTGTGGTTTGTGTACCTAACATGTACTTACTAGTATCGTTTTTTTGTAGAATGTTTTGAACCACTAACAATGTTTTTAGCGACTAAAATGCTGTTTGACTCCGAGTGGAAACCGATTATAAATCGCGTTTCCCTGTAGCCATCTGCACTTTTAGTTGGACTGGTTACATCAAATCTTTCAGGTAACCAGAATATTAGCATGCTGAGGGACCTGAGAACCAGGTTCCTTTTTCTTTTTTATTAGTATTCGAAAGTGTTCAAGTATATTATTAAACGTTTGGGTTTGGCAGTGTTAGCGATGTTTATCGTGATGACCATTGTCTTCTTTTTGGTTAACTCCACAGGTCAAACGCCCTTATCAGCAACCTCTTCTAAGGATTTGGAGGCTGTCAAAACACAACTAGATGCCTTTGGTTTTAACGATCCGTTAATTGTGCGATATGGTCGTTACTGACAAACCCTCTTTTCAGGTAGTTTGGGTACTTATTACAGCTCCCCGAACCAAACAATTGACCAAATTGTCTTTGGTCGGGTACCTAACACCCTTTACGTTGTTTTAATTTCTTTCTTTATCGGCTCTTTGTTGGGCATTATCTTCGGGATGATTTCCGGATTATTCCGTGGCAAGTTAATTGATGCGGTAATTAACGTGCTCGTTGTTTTATTTGTTTCGATTCCTTCCTTTGTGGTTGGTTTAGGTTTACTTAAGGCAGCTGGTTTGTTCCGTTTACCACCGCGCTTTATTAACTTCGATGATGCTAACTTTAACTTTGGTAACTTCTTGTTAGCCTCCATTATTCCTATTTTATCCTTGGTATTTTACACCTCAGCGGCCTTTACTTATCGGGTACGGAATGAGGTCGTGGAAGTAATGAACCAGGACTACATAAAAACAGCACGTAGTAAAGGTTTGAGTACTTTTGCTGTAGCGCTTTACCACATCTTCCGTAACTCAATTATTCCTTCTGTACCTTTATTTGTCTTTGGGATCTCTGGTGCCTTTTCGGGTGGTTTTATTATTGAATCCTTATTTGGTGTTCAGGGGGTATCACGGATCTTAATTGACTCGGTGCAAAGTAACGAAACTAACCTCGTGATGTTTAACATTATGTTCATCCAGGGGATTCCCTTACTAGCAAGTGTCTTCATTGAGTTGATTTATGTCCTAGTGGATCCTAGGATTCGGATTGCTAGTGCCGGTGGGGTGAGTCTGTGAACTAAGCTTAAGTTTGTTTATTTACGCCAAGCTTGATTGCGTAAGTGAAGACGGATTAACCACACTAATTCCCACAATGTGTTGTTCAATTCGCCCCAACACCGCCAGCTGTTAGAGCTCAAAGCAATTGATTACAAGCACAATACCATTAGCTTAACTGAACAGCAAAAAACCACCCTTAAAATTGAACCAACTGCTAACTTTGTTTTGTTAGGAACTAAATGTTTAAAGATTATTACGATCCATGGATAAGCACCAAAAATTTGACCAAAGCCTGTTTCAACGGGTAGACATTAATGTCTTAAAACGCTCTGACCAGTTGATTGGCAAACCAACCACTAACTTTGTCGAAATTATGAAGCGGTTGTTCCAAAATAAGTGAGCAATCCTCTTCTTTTTACTGATTGTGCTAATTATTTTGTTAGCCATCATTGTACCCTTAGCATCACCTTATTCAGCCGTTACTCCAGTATCGAACAACGCTTTGGCACAGAACCTACCACCACGATACCTCTGGAACGGCGCGGGTGACATTCAAGTAGAGAAAATTACGGCACGCTCGATTGCTGAAGTGGCCCAATCAAGTGGTGTTTTGGTAGGGAAATTACCGGAGGCAAGTAGCAATCCACTCGCTACTAACGTTAAGTACAACATTGCGCCTTACCAACTAGCAGAGCTGAAAAACTACTATCCATTATTAGGCACTAACGGTTTAGGGGTTGATATTTGAACTTTACTGTGAGCCAGTATGGCCAAGTCACTCTGAATTGCGATTGTGGTAGCCTTAGTTTCAATGGTCTTCGGCACCATTTACGGCGCGATTGCTGGGAGCTTTGTTGGACGCGCTGCTGACAACATTATGAGCCGGATCATTGAAATTATTGATTTGGTTCCTTCAATTCTCTGGATCATTGTCTTGGGAGCAACATTCCGCTTTGGTGGGGTAAAACAATTTGATGACAGTGTGGTGATCTTTACCTTAATCTTTGTCTTTTGGACCTGACCCGCTGCTACCACAAGGATCTACATCTTAAAGAATAAGGATACGGAGTACATCCAAGCAGCGCGTACCTTAGGAGCAAAACAAATTCGCATTATTTTTGTCCACATGCTACCAGTTGTAACTGGTCGTTTAGCAGTCGTCTTTGTGAGTTTGATTCCCGCTGTGATTGGTTATGAAGCATCACTGGTTTTCTTAGGGCTTAAACCTGCTACCGAAGTGGGACTTGGTGCTTTATTAAACCAGGTCACCAGTAGTGGTAACATAGCACTGATTACCAGCTCAATTGTGAGCTTTGCAATTCTCACCGTTTCGACCCGCGTGTTTGCTAACGCTTTAAATGACGCAATTGACCCACGGGTAATAAGGAGGTAATATGGCACTAAAAGCAACTAATTTTTTTACAGAACCAGAGTACAAACTGCAGGATGATTTGATCTTAGACATCCGTGATCTTCACGTGAGTTTTAAGGTTAAAGACGGCATTATGCAAGCTGTCCGTGGTGTTGATCTCAAAGTCAAAAAGGGCGCCATTGTCGGCATTGTGGGCGAATCTGGTAGTGGTAAATCGGTCTGTGTTAAGTCGATTATTGGCTTTAACGATGGCGCAAAAACGACCGCTAAGTTAATGAACTTTAAGAACATTGACATTAGCAAGATTAAAAAGCACCAGTGACAGTACTACCGTGGCACGTACGTGTCTTACATTTCCCAAGATCCACTGTTCTCGTTAAACCCCACCATGACCATCGGTCGCCAGGTTAAAGAAGCGATTTATGTGTCGTGTAAGCGCCGATATTACCAAACTAAGAGTGACCTCAAATACGATTTGCAAACAGAACGAATTGACCTGGACACTTACAAGGAAAAACTAGCACAAGCCAAGGAAACTTACAAAGCTAAAACGACTAAGGCGGCGGTCCATGCAAAAACGCTAGAAATCCTTAACTTCATTGGCATTGACCAAGCTGAAAAGCGCTTAAAGGCCTTTCCAAGTGAGTTTTCCGGGGGAATGCGGCAACGGATTGTGATCGCGATTGCCGTAGCCACCGAACCGGATTTAATTATTGCCGATGAACCGACCACGGCGTTGGATGTGACCATCCAAGCGAAGGCGTTGAACCTAATTAAACAGCTGCGTGACCTGCTCAACATTACCATTATTTTTATTAGTCACAACATCTCGTTAATTGCTAATTTCTGTGACTTTGTATACGTAATGTACGCTGGTCGATTAGTGGAAAAGGGCTTAGTTGAGGAAATCTTTACCAATCCGGTGCACCCCTACACTTGGGCATTAATGGCTTCTATCCCTGAAGGTACGGACAAAAACGCTCCGCTAACCTCAATTCCTGGGTATATTCCCAATATGTTATCCCCGCCTAAGGGCGATGCCTTTGCAGCGCGGAACCAGTTTGCACTTGCCATTGATTTCGAGCACCAACCGCCGTTCTTTGATGTAACGGAAACTCATAAGGCCGCTACCTGGCTACTCCATCCCCAAGCACCGAAGGTAGAGATGCCCGCCGATGTCAAGGAAAAGATTGCCATTACCCGCAAAGCACTCGCCATTAAAATGCCATCTCAACCTGTAAAACAACCAAAAAGCAATGGAAACAAAAAACAAAAACAAAATCCGCTCGTTAATGTTAAAGCCTTGAGCATGTTGTTCAAGGTCCGCGGTAGCTTCTTTAAAGCCTTAGATGAAATTGACTTTACCGTCAATGAAGGTGACTTCTTTGGGGTCATTGGCGAATCGGGTAGTGGTAAGTCAACCACGGGGAAGTGTTTAATCCGGTTAAATATCCCGAGTGGCGGTAAGGTGGAAATTGCTAACCACCTTATTTCTGGTAAAAAACTAACGCGCGAAAACGACCACTGGTTAAAGCAAAACGTGCAGATGGTCTTTCAAGACCCATACTCTTCTTTAAACCCTACCAAGAATGTTTTAACGGTCATTTCCGAACCGTTAGTAATTACCAAAACGGTGTATGGTGAGGTAAAGGAATATCTTAAAACGCTCGCTAAACTGTCCTTTAAAACCAAGAAGGAATTGTTAAGGGAAGACTTTGAACTGGAAACCCAGTTTTACGAAAAGTTCTTTTCAAAGGTACTGTTTCACTTAGAAACTACGATTAACAAGTTTGCCCTACTCCAAGAGAGTAATAACAATAGTTCAGCCGAGTTAGCACAAACCATTTTGGGTCACACTGATGATCTAATTGAAGCATTACGGCAAGAGTTCGGTCTGGTCTATGAGTTTTCCTCATCCCAAAGCGAACCGCTACAAAAGGCCCTCAAAGACAAACAGGAAACCTTAGCACAGGACACGATTGACAAGTTAAAGCAAGAGCTGTATACAACGCAGCAAAAAGCTAAAGTTTCCACCCAGGCCTTTGCTACTTGACAAAAGTTGCAACAAACTAAGCAGAACTTAAAAGCGTACCGTGCCCAAATGGCGGAAGAGTTGCAAAACAAACCACGGATTTACCTCAATGCATGGTTGTTAACAACGAAGAACTACATTAAGGATTCACGCCAAAACACGCAGTTAACTGACGATGTCTTTGCTTTTTCTTACAACGACATGGTCGACAAAAAGCGCCGCTTAGTGTTAGTGTTATCGGAGTATTACAAGGCGTTGCCGTTCTTTTATGACAATTGGATCCACCAGAACGCCGATCGTTTTGATGAATTGACCAACGCAGTCTTCTTTGACTTGATTGATGTGGTGATTGCCTTAAACCGTGATTTTGCTAATGTGGAGAGTGACGCTAAGGCCGAGTTAATCCGTTTTGTTCAGTTTATCCGTCGCTTGTGTGACTTACGCTTTGCTGCTTTGAAAAAGAGTTTTAAGAAGCAAACTAACTATAGTTTTGACTTTAACCGCGAAACCGAGTTGTTATACGCCAACAGCTGTTATGACATTAAGGAGCTCCCCCAAGTAATCCAACCATACTGGGAAAAGCTCTTTAGTGATGCTAACTATGACAAGATTGCCAAATCAGTGCAGGAACTCAACGATATTATTAGCACTGACATTGAAAAGGCATCGAATATAGCCAGTGAAATTAATACCAAGATTAGTAGTTTTAAAACTGAAATTGCGGAGTTAAAAGCTACCTTTAAAACAGAAAAAAAGCAGAAGATCACAGTGCCCAAATTACTGGCTTAAAAACGCAAATTGCTGAAATTCAAACGCAAATCAAGCAGCAAAAACGCGAAGTCCAAAGTACCGAAAAGGCCGCTTTAAAACCAGTATTAAAGCAGTACAAGAGTGCGCTGCACCTCTATAAACGCTTTAAACAGTTGTTACGCCAATTTACCAAGCAGTTAAACCTCCTTGTCAAAAAACAGCAGGAATTAGAAAAGATTGAGGAAGGCTTGGATCTAACCATTTGAGAGCGGATCCAACTGTTGTTCCACCCGGTCGAAGGTGATCTCAAGAGTGAACTCAAAACACGCTTAAAGTCGTTTGGGGTGATTAACTTTGAGTACAAACGGGCTGTGCGTGAATCCCGTGTCTTTCGCTTAGTCCACTTTGGTCATGACGTGATGAAGTGAGGATTGTTCTTACCATTGACCAAGATCTTCATGCGTAACAAGGTGTATGAAGCATTAGACAGTGTGGGCCTCAAACGCGAGCACGCTTATCGCTATCCGCACGAGTTTTCTGGGGGTCAGCGGCAACGGATTGCGATTGCCCGCGCTTTAATTACCAAACCTAAGTTAATTATTGCAGATGAGCTTATTAGTGCTTTGGATGTATCCATTCAAGCGCAGGTGATTAACATCTTGAAGGACTTGGCTAAAAAACACAACTTAACGGTGCTCTTTATTGCCCACGACCTTTCGATGGTACAAACAGTGTGCAACCGTTTAATTATTATGCACCGCGGCAAAATTGTGGAACGGGGTAGTACGGATGAAATCTTCGCGCACCCGGTCCACCCTTACACCCGTTCCTTGATTAAGGCATCGCCAAAGTTGAGTAAGATTAACATTGACCTAGCCTCCTTTGACGAGAAGTTTACGTATGACAGTGATTATTCACTCACGAATATGCCAAGCTTCTTGAAGGTGCCAAATACCCAAGAACACGAGTTGTACTGTACTCAAGGCCAGTTTGACAGTTGGATCAAAGGAGCAAGTCGGATAAATTAATTTAAAATTTCTAGCAAATTGTGGGAGCTAGTTTAGCGTTATTACCACACCAATTATGGCAAAAAAGACTATTACAAGAATCGCTAAGATTAACCTCTTAGGCGGGCAAGCAAAGCCCGGTCCCGCACTCGCATCTGTTGGGATTAACATGGGTGAATTCACCAAACAATTTAACGAAAAAACCAAGGACAAACAGGGCGAAATGATCCCCTGTGTGATCACCGCTTACAACGATAAGTCGTTCGACTTTATCTTAAAAACTACCCCAGTTAGCATCTTGCTTAAGCAGGCTGCAAAACTGGAAAAGGGGGCGAAAAACGCCAAAACAATTGTGGGTAAGATTACGATGGCCAAGGCCAAGGAAATTGCGCAGTACAAGTTAGTGGATCTGAACGCTAACACCGTGGAAGCAGCCCTTAAAATGGTTTTAGGTACTGCTAAACAAATGGGAATCGAGGTGATCGAATAATGGCTAAACTATCAAAGAAAATGAAGATAGCTGTTGGTTTGGTCGATAAAACCAAGCTGTACCCGCTCCAAGAAGCAGTTGATTTAGTCAAAAAAACTTCAATTACCAAATTTAATGGCTCAGTTGATATTGCCGTTAGCCTTAATTTAGACACCACCAAGGCGGAACAACAGCTCCGCGGTGCGATTGCTTTTCCTCACAGTGTTGGCAAACCAATCCGTATTCTAGCGATCACCGATGATGAAAAGGCGGCACTGGAAGCAGGTGCTGACTTTGTTGGTGGCATTGACAAGATCAATGACATTAAAAACGGTTGACTAGACTTTGACCTGATCATCACTTCTCCAAAGTTTATGGCCGCATTAGGTAAACTCGGGAAGCTGTTAGGTACCAAGGGTTTAATGCCTAACCCAAAAACAGAAACCGTTACTGATGATGTTCCAGCTGCTGTACGCGCTTACAAGAAGGGGAAAAAGGAATACCGCGCTGACAGCTTTGGTAACATCCACATGTCCTTAGGCCGCGTGGACAGTGCGTCCAACCACTTGGTTGAAAACGCTTTGGCACTGCTTGATCTCATTAAATCACGTAAACCAGCAACCGTTAAAGGAATTTACATTAAGAACATTGCCTTGACCACTACCATGGGTCCGAGCTTAAAGGTTAAGCTCCCTGATTAATGGACAAGTTGCGCCTCGTTGTTGGTTTAGGTAATTTAGGTAAGCAGTACGCTGAAACCCGTCATAACGCGGGGTTTAAGGTAATTGATCGTTTACTAAGCCTTTACCATGTCCAGTTAGAGGAACGCAACAACTTAGGTGAATTCATCCTTTTAAGAAAACATAAGGTAGTGTTAGCTAAGCCAAACACTTACATGAACCACAGTGGTAAGTTTGTTAAATGAGCTTGTCAAAACTGAAACATTAAACCAGATAAGGTAATGGTGGTTTATGATGAATTAGCCTTCCCGTTAGGTACTGTTCGACTAAAAATGCAGGGTTCAGCCAACAATCATAATGGCATAAAATCAGTAATTGCGCATTTAAATACTGAACACTTCAACCGCCTCCGGTTCGGAATTAAGTCCGATAATACTAGTAACATTCTCCATGAAGTGGTAATGTCGGAGTTTACCCCAGCCGAGCGTAATTTACTTGAAACAGCGCTTACAAAAGCGATTGAAGCACTCAAGGGTTACATTGATGGTGTGACAATGCTTAAATTAATGGAAGTTTTCAATGCCTAAAACACAATACATAGCTGGAGTATCAGGTGGTCCTGATTCAATGTTGTTGTTAAAACTGTACCACAAGAAGATTGCCTGTGTTGTACACGTTAACTACAATAAGCGGACTACTGCACTCCGCGACCAAAAAATGGTCGAAGAATACTGTAAACAGCTCAAAGTGCCCCTCATTGTGCACACGGTACCTGAAGATACCGTTTGAAAGAAAAACTTTCAAGCACAAGCTAGAAAAATTCGCTTTGAACAGTTTCAAAAAGCAGCTAGCCTTTATAAAGTAGATAAGCTGCTGTTAGCCCACCACCGCGATGATTTTATCGAACAGGCCAAAATGCAGTTGGACGCACGCAAGCGTGCCATGTATTATGGCATTAAAACACGTGGCGAATTGTATGGGATGAAGGTCTATCGTCCCTTTATTAAGTACTGAAAAAACGAGATCTTAGCCTTGTGTGAAGAACACAAAGTGCCTTATGGTATTGATGAAACCAACGCGCAGCCAATTTACAAACGTAATCAGGTACGCCAGGAGATCGCTAACTGATCGAAGGAAGAGAAGGAGGAGTTTTACATTGGTGTGTGCGCCATGAACCGCGTCATTGGTCAAAAGCTCTTTTCCTTAATGAAACGTGCAAAACAGTGGTTAGCTCATCCTGATGTACGTGAATTAAAGCGTTACCCACTTCCCGATCAACGTCAGTTGGTTTACAGCTTTTTAATTACCCACCACATTGATGTAACGGGGGACAAGATTGATGCCATTTTAGACTTTATCCAACCGTTTCAACAAAAGCATTACCGTCTCAAGGATGAGATCTTCTTATCCATTAAAGACGAACGGCTAACTTTACTGTACAAGAGTTAGATGAAAAAGTTATTAGTCAAGGAGTTAATCGAACAATTTCAGGATTGTGTTAACCTCATTGATGGGCACACTAACACCAGTAATGTAATTAGAGTACCCGGATTAAAGCGGGTGGTGTTTGAAATGCTGGGGTTGTTTTCCAGCCAAATTGGCTCGGTAGCCATTTTGGGCAAACGCGAATTTGGCTTTTTGAGTCAAAAAACGCTGGTTGAACAACAGCAAATTCTCCACAACTTACTCAAACTCAACCCACCAGCGATTATTTTGACCAAGTCCTTTACTGACCCCACGGTACTTTTACAGGTTAACCAGACTTATCAGGTACCGATCCTAAAGACCGATTTCTTTTCCACGGAACTTTCCTTTACGGTCGAAACCTACATTAACGAACAGTTTGCTACTGTTGCACAAATTCACGGCGTCTTGCTGGAAGTTTTTGGTGTAGGTGTCTTATTAACTGGTCGCAGTGGCATTGGTAAATCGGAGTGTGCCTTAGATCTAATTAACAAGAACCACCTCTTTGTCGGTGATGATGCGATTGAAATATACCGTTTGGGTAATCGCTTGTTTGGTCGCGCGCAGGAAGTAGCGAAAAAGTTTATGGAAATCCGTGGTCTTGGTATTATTAACGTCGAGCGCTTTTACGGATTGCAAATTACTAAACAGCGCACCGAAATCCAGTTAATGGTGAACTTACTGAGTTTAGAAAAACAAACGACCGTGACCTTTGAGCGTTTAGGTACCGAATTGAAAAAACAACGTTTACTCGGAGTTGATTTGAGCTTTTATGAAATTCCGATTTCCCCCGGGCGGAAGACGAGTGAAATCATTGAAAGCGCTGTAATTGATTTTAAACTTAAGCACAGTGGCTATAACTCTGCTTTAGACTTTATTGAAAACCAGAAGGCCATTTTAAAACGTAAAAAAGATGAATCCTAGTGTTAGTAGCAGACCACCATGATCAACGGCCTTTTACTTAGGGCCGGGTTTTCCTATTCAATGGTATGGGATCATAGTCGCAATTGGGATTGCCTTTGGCATCTTAATGTTTGTCTTGAAGTTAATTTACTTTTACAAGATTCAAGATAACAGCTTTTACTTCTTTATCTTCATTGCAGTTTTAACAATGGTATTGGGCGCACGAGCTTGGTATTTTTTGATTGAAGCGGTTGATGGTCGTTCAAGTGGGAGCAACTTCTTTGACTTCCGCAACGGTGGGTTAGCAATCCAAGGTGGTGTGTTGTTAACTACGTTAGCGGGGATTATCTACTTTAACGTCTTTTTAAACATGAAGACGACCAAAACCAAAACCACCGCTAAACTATTAAACAACAAGAACCAGATTAAAACGGTTTATGTCGAGCGTAACATCTCAGTGTTTGTGATGTTGGATCTCATCGCTCCCTGTGTGTTAATTGGTCAAGCCATTGGTCGTTGGGGTAACTTCTTTAACGCGGAAGTTTATGGCGCAGCTTTAGTGGGTTCCAAAAATGATACCCTGTCAGCTGCTAACACCACCTGGGGCTTTTTAAGAATCTTAATGCCCAAAGTGTGGGACGGAATGTTTATCAATGGTAGCTTCCGCATTCCGCTTTTCTTAATCGAATCGTTCTTTAACACGATCTTCTTTGTCTTTATCTACTTTGTAATGGACCACATTAAGGGGATCCGCAGTGGGACAATTGGCTTTAGTTACTTCTTAGCGACTGGCATAGTTCGCTTAATCTTGGAAACGCAGCGTGATGAAGCGTTTAAGTACAATACCTCGATTGTGTTTAGTGCACTCTTAATTTTGGTGGGAATAGTAGGGATTATTTACTGTCAAACGTTAGCAATTAAATTGCGTGGTTATTTCTGAACTTACTTCTTCCTATACGGTTGGTACAAGGTAGCCGCTTTCTTTACTACCTTATTTATGAAGGATAGGACACAGGCTTGTTCTAGTAAGTTTGCATTTTACGAAAAGAGCTTACCTGAAAAGGAACGTTCCTTCTTTCAGTTGAAGTACTACAACGATGTTTTACCACCGAAAATTTACCGTTTGTACGACCATGAAATGCTCATGTTTGACAAGCTAGAAGCAGTGCCGGAAGCTTAGATTGTGTTACAATATTCGCGCTGTCTTTATGGCTACAATCGCACAATTAATTAGAAAACCGCGGAAAAAGAAGAAGGTTAAATCTAAATCACCCGCTTTACATTACAACCTCAATTTACTCAACAAAAAGGTAACCAACGTGTATTCACCACTTAAACGTGGTGTGTGTACCCGGGTTGGTACCATGACTCCAAAAAAACCGAACTCTGCTTTGCGCAAATATGCCAAGGTAAGACTTACTAACGGTTTTGAGGTGTTGGCTTATATCCCTGGTGAAGGACACAACCTCCAAGAACACAGTGTAACGCTACTCCGTGGCGGTCGGGTGAAAGACCTCCCTGGTGTGCGTTACCACATTGTTCGTGGTACCTTAGACACTGTTGGTGTGGAAAAACGCCGTCAACAACGTTCAGCTTACGGGGCTAAGAAACCAAAAGCCAAGTCCTAATATCTTAAATTCCTTTTTTTACGAAAAACATTAACTCAATCAATTAACTTTATATGCGAAAAAATCGTGCTCCCAAACGGACTGTTTTACCCGATCCCGTTTTTAACAATACGCTGGTAACCCGGATTATTAACGTCATCATGGAAGATGGCAAGAAGGGTTTAGCACAACGAATCTTATACGGTGCTTTTGATCTCATTGAACAACGCACTAAAGAAAAACCCTTAACCGTGTTTGAACGCGCTGTGGACAACGTGATGCCGCGCTTGGAACTCCGGGTACGGCGGATTGCGGGATCCAACTATCAGGTACCTACCGAAGTACCTCAAGACCGCAAGATTGCTCTAGCACTCCGTTGAATTGCAATGTTTGCGCGTAAGCGTCATGAAAAAACGATGTTAGAGAAGATTGCTAACGAAATTATTGATGCATCGAATAATACTGGTGCAGCCATTAAAAAGAAGGACGATACGCATAAGATGGCTGAAGCCAACAAGGCCTTTGCCCACATGCGTTGGTAATAGAGAATAGAGTTAGTTATTTATGGCGCGTACAGTAGATTTAATTAACTTCCGTAATTTCGGAATTATGGCCCACATTGATGCGGGTAAAACCACCACCTCAGAGCGAATTTTATTTCACTCAGGCCGAATCCACAAGATTGGGGAAACCCATGACGGGGAGTCCGTGATGGACTGGATGGAGCAGGAAAAGGAACGCGGTATTACGATTACCTCCGCAGCTACTTCAGTTAGTTGGAAGAACTGCAGCTTAAACCTAATTGACACCCCCGGTCACGTTGACTTTACCGTAGAAGTAGAACGCAGTCTCCGTGTCCTTGATGGTGCCATTGCTGTGTTAGATGCTCAAATGGGGGTAGAACCACAAACCGAAACGGTGTGACGCCAAGCATCGCGTTACGAAGTGCCCCGGATTATCTTTGTCAATAAGATGGACAAAACGGGGGCTAACTTCGAGCGTTCTGTACAATCGATTCAACAGCGCTTAGGAGTAAAGGCGGTACCTATCCAATTACCAATTGGTGCGGAAAACGACTTTAACGGCATTATTGACCTGATTGAAGAGAAAGTTTACTTCTTTGATGGTGGTAAGGAAGAAAAGGCCGAGGAAAAACCAATTCCCGACCAGTTTAAGGATCAGGTCAAACAAATGCGTGCGCACCTAGTGGAAGAGGTGGCTAACTTTGACGATCAGTTAATGGCTGATTACCTTGAAGGTAAGGAAATTAGTATTGCCGACATCAAGCGCTGTATCCGCAAAGGGGTAATAGGTTGTCAGTTCTTCCCGGTGCTTTGTGGTTCTGCCTTTAAAAACAAGGGGATCAAACTGTTATTGGATGCTGTAGTAGATTTCTTACCTTCCCCAGTTGACGTACCGCAAGCCAAGGCTTATGGTGAAGATGGCAATGAGGTGTTAATCTCGGCTAGTGATGATGCACCGTTTGTTGGTTTAGCCTTTAAGGTAGCGACTGACCCATTTGTGGGACGTTTAACCTTTGTCCGCGTTTACTCCGGAGTACTTAAGTCCGGTTCTTACGTTAAGAATGTAAGAAAGAACAAAAAGGAACGGGTATCACGTTTGGTAAAAATGCACGCGCAGAACCGCAATGAAATCGAGGAAATTCGTGCTGGTGATATCTGTGCTATTATTGGATTAAAGGACACCACTACTGGAGAAACCCTAGTTGATGACAAGATTGATGTCCAACTAGAAGCGATGCAGTTCGCACAACCCGTGATCTCTTTAGCGGTGGAACCAAAAACCAAGGCAGATCAGGAAAAAATGTCGATTGCACTCTCGAAGTTAGCGGAAGAAGATCCGACCTTTAAGACCTTCACTGATCCCGAAACTGGGCAAACGATTATTGCGGGAATGGGTGAATTGCACCTAGACATCCTAGTGGATCGGATGCGCCGCGAGTTTAAGGTTGAAGTTAATGTGGGCGCACCACAAGTAAGCTTCCGTGAAACCTTCAATAAAGAAAGTGAAGTTGAGGGTAAATACATTAAACAATCGGGTGGCCGCGGTCAGTATGGTCATGTCAAGATTCGCTTTGAACCAAACAAGGACAAGGGCTTTGAGTTTGTCGACAAGATTGTTGGTGGTCGGATTCCGCGTGAGTACATTAAACCAGTGCAAGCGGGTTTGGAAAACGCTATGGCATCAGGTCCGCTAGCTGGTTATCCGATGATTGACATCAAAGCTACCTTGTTTGATGGTTCGTTCCACGAAGTTGATTCGAGTGAAATGGCCTTCAAGATTGCGGCATCCTTAGCTTTAAAAGAGGCGGGCAAGGTATGTAGTCCGGTACTGTTAGAGCCAATTATGGCAATTGAAGTGACGGTGCCAGAACAATACTTTGGGGACACCATGGGTGACATATCATCGAGAAGAGGTCTCATTGAGGGGACTGAACAACGTGATAACGTGCAGGTAATTAAGGCCAAAGTACCACTCAAAGAGATGTTTGGTTACGCCACTGATTTACGTTCCTTCTCTCAAGGTCGGGGTAACTATGTGATGCAGTTTAGCCATTACGCTGAAACCCCTAAGAGTGTGGTGAATGAGATAATTGCCACCAAGAAATAGCAAAATAGATAGGCATAAATCTTTACTTTTATATTTAGTTTTCCCATAAAGTTACGATGCAATACAACATTATTCTTTTAGTTGATGGTTCGCTCTCGTTAGAGCAAGCTAACCAAGTGAATGAAAAACAACAGCAAACGTTAACCAACGTTGAGGGTTTGCAAACAGAATACCTTGGTTTAAAGGAGTTGGCATACCCAATTAAGAAACAACTCTCAGCGCATTATTACCGATGGAAGTTTAGTGGTGATAACCAATCCACGAAGGACTTTAAGCGTACCGCTAACATTAACAAGCAAGTATTACGTGAACTGATTATTAACTTAGAACGTGAGTACGGCTACTTAGCTTCGATAAATCCAAAAAAGCAACAGCTGGCACTGCAAAAACGCGCTAAGTATGACGAAATCATTGCGCGGGAAAACAACCCCGAAAACCCGGATGTACCAGTAACATCAGGTTTAGCTTCCACCCAACCACGCTTATCACGTACTGAAAAGGCACAAAAACCAAAGGAAGAGCTGTGGGATGTGGTGCAAAAGATGGGAAACTTTGACAGCGTTCAAGCCAACCCATACCGCCCACGTTTCAAACGCTTTAACGCGGAACACGTTAACCAACGGCAAAACCAACAAAACAATAACAATAACCGTTTTGACCGCAACCGCAACCGTCAGCACAACCGCTTTAAGGATAAGCAATAATGAACCGCGTTTTTTTATTTGGTAAGCTCTCGTTTGATCCTAATAAATTGCAAACCCGTACCAACAATATTGGCGCGTCCTTTTCCTTAGCGTGCATTGATTCGAGCGGTTTTAATGACAGTAAGTCCTATATTAGGATTACTGCTTGGGGTAAAGTTGCTAGCTTTGTTTTAACTTTAAAACCCGGTGACAGTGTTTTTGTGGAGGGGAGATTGTCCACTTACAAGATGAACAACCGCAGTGATGATCCCAATTCGAAGGCCACTTACGCGCTCCAAGTTATCGCTGATAAGGTTTACCGTCCCGATGAGGAAAACAGTTTAGAGCAACCTGTTGATAAGGCTACGGTAATTGATTCACCCTTTTTAGCAGCGAAAACCAATGCTACTGAAAATGAATTAGCCCAAGCATTTCCGATTAGTCTGGATGATGAAGATGATGACATTAACCCCATTTTAAACAACGATTCCCAACTAGAGGAGGAGAGTGATGATGAATAACGAACACGATAATTTCCAAAAAGAAGTTGAAACTACAACTGAAACAACCTTCAACAGAGAAGAAGGCAAAAGAATGGTACGTCCTTTATTTAAGCGTTCTAAAAAGTACTGCCGTTTTTGTGCCATTGGTCAACTTAGAATTGACTTAATTGATGATTTAGAAGCGTTAAAGCGTTTCTTGAGTCCCTACGCTAAGATTAACCCACGCCGGATTACTGGTAACTGCCAAATGCATCAGCGCCATGTAGCCAAGGCCTTAAAGCGTGCACGTTACTTAGCCTTAGTTCCGTTTGTAAAAGATTAGTTATGAAGGTAATTTTGAAGCAAGATGTTAGCAACCTAGGCAAACGCTTTGATGTGGTTGATGTTAAAGATGGCTATGCAATCCACTTTTTGTTTCCTAAAAAACTGGCTGCACCTTTAACGAAAAAGAGTTTACAAGACCGTGATTTGTTCTTAAAGAAACAACAAGAACACTATGAGATTAACAAGGCGTTAAGCCACAAGTTAAAGGAAGTAATTGAACAAACAGAACTCCACTTTAGCTTAAAGGAGCACAACGGTCGGCCATATGGCTCGATTATTACCAAGCAAATTATTAACCAAGCCCACACTAAAGGGATGGCGCTGCAAAAGTTTATGTTTAAAGACAACGTGCGCTTAGGGTTTGGGGATCACGAGATTACCTTACACATCTTTGAAGATACAACCGCAGTTTTAAAAGTTAAGGTAACCCCGGATAATGGTGTCAAATAAGCTTGAACAGACCTTTAAGTTTGCCAACGACAAGAACATTGAACGTGCTGAGCGTGTTTTAATGCAAGCTTTAGTACAAGATGCCGTTGGTGTGGACTTAATCTTAACCAAGCTTGAACCGAAGGACTTCTTTGGTTTTCCGTTTAACTTTATCTTTCAAACAGCACAGGAAAACTATTCTGAGGGCAATAAGTTGTTTGGTTCGGCGCTTTTAGAGGCCGTTAAGTTTAAGCTGGATACCGATTCCAAAACACAAAGGGAAATTGAAGGTTTATTTGAGGACGTTTTACTCAAAGGTCTTCCCTTTTTGGAAAGGGACATCAAGGTCTTTGTTGATGTTGTCAAAAAAGCTTCGATTTTTCGCCAACTTAAACAGTTCGCCAAAAAGGTTGAAAAGGAAGAGTTTAAGGTCAAAGATGACCGTTTTGAGGGCTATTTACAAGCAATTCAAAACGACTTTACCAAGATTATTCACAGCGCCTTTGTTTCTATTCCCGGTTTGAGTTATGACGAAATTGGTCGTGAAGAGGAAGAGTTAATTCGCAAAGTGTACCGTGGTGAATTAACTGTAAAAGGGCTTAAAAGTGGTTTTTACCCTTTAGATCAATTAACCTCTGGTTGAAAAGAGGGTGAGTTAATTGTGGTAGCAGCGCGTCCAGGTCGCGGGAAAACAGCGCTTCTAATTAACTTTTTGCAGGGCGCGGTTAATGACAGCGAAAAGTTTGATAAAAACAAGGATGTGTTGCTGTTTTTTAGCTTAGAAATGCGTAACCGTGAAATTTATCAGCGCCATTTAATGCTAGAAAGCCAGGTAAATTACACACTCACGAGTCGTCAAAGGCTCGCTAATGTGTATGATGATTTAATTAAAGCATCAGAAGTATTGCGCGATCTTCCTATTAAAATTTTTGATTACAGCACGATTACGTTGGACGAAATTCGTGCTCAAATCACGGAAGTTTCAAAAACCAATAACGTTAAGTTGGTCGTAATTGACTATCTCCAACTAGTTAATGCATTTAAAAACAGCTATAACATTTCGCGTCAACAAGAGGTCACCATGATTTCCAAATCACTTAAAAGCTTTGCCAAGGAGTTTAACGTACCAATTATTGCCGCTGCACAGCTTTCTCGAAGAATAGAAGAACGCAAAGATTCGCGTCCCATTTTGTCGGATTTACGTGAATCGGGTTCGATTGAACAGGATGCTGACATGGTTTTATTCATCCACCGCGTCAAGGATGAGGACAGTGAAAATCAAGAAGCAATTGGTCACAACAATATTTTTCAAGTGGAGTTAATCTTGGAAAAAAACCGTAGTGGTCCGACTGGTAAAGTACAGTTTGACTTTCAAAGTGACGTTTCTACCTTTCGGGTTCGGGAATACAATCCTGATGGTTACAGTTAGTTTATGAAGTACAAAACAGTTAAATCGATCCCGCTTTTTTTGTTAGGCTCGATTGTGTTCACTGCTTGTTCAACACCACAATCAACCTTTCATTTACCTGTGCAAACGACCGTTAGCGCAATCAAAAAAGATATTAGTGGGAAAACAGCTACTGCTGTTAAGGCTGCTAGTAGTTCATCTAGTACTACTACATCTAACGATGACAACAACCAAAAGGGTTACTTCTTAGAAACTAATCGTTCCACCGGTACTTACGACCCTAACAATTCCACCCGGTTAATTAAATTAGGTGAAAGTGGTGATTTTCATGCAGCTGACCAGAATAAACCGGAAGAAGCCTTGTTTGAACGACTTTACGGCGGCATTGCTTCCTTATTAAATTTCCGCATTATTAAACCTGCTTTAACTTATTGAAACACAGTAACACCTTCTCTAAAAGCGATTGGTAAAAGCAGTAATCTAATTACCTTTAGTCAAGATATTGATGAAACGGAGTTACAACGGGCATTGGCTAATAACTTAATTGTCGCTGATGATGGCAACAACAACTTTTGGTTTGGTTTAAAGTCACTTAGCTTTAATGCAGCTAAGTTGACTGACAGCGCCCAAACACAAATGGCGCAAAAAACCACTCAAGCAGTTACCTTGAAAAGTCAAGCGCAAATGTCAAGTACAAATACCAAAAACACCAACAAAAAGATTGATTTGCGCGATAAGATTACTTTATCGAGTACGATGAATACTCAAGGAAGTGGTGATAACAAAAACCCATCTAGTGGTCTTATTCAAAAGTTAGTGAGTGTCGAAAATATTGAAGCTGAATTTAGTTTTGTTAAAACTGGCTTCAACGGTAATGAAATTAAATTTGGCGATTTTGTAACTGAGAATTCACCCACAACCACTCAACTTAAGCAAGTTTGGAAAAAGAAGTGGGGCACGGAATTGAAAAAGACAAACTATAAGCTTCAATTGAACAACTTTAGTCTATTGCTAACTTACACACCAGAAGTTAATAAAGTTGAAAAAGGTAACAATGGTGATTCCAATAAAGGCACGATCGCTACGCCAAACGGTTTTAGCTTCTTGTACCCAGCTAATTTAAATGAGACACCAAGCAGTAGCAGTAGTTATTGAACTAATGTTACTGACTTAACTAAAGCAGCTACTGACACCGAAAATACCAATTTACTCAACGACTTGCAAAAGAGTCAGGAACAAGTCAATCAATTTGTTGCTGCTATTACCCAAAATCACCTCGATGTTTCCGAAGCTGCTTTAACTAAAAAACAGTTTGGTAGCCTTAGCATTAGTGATTTCTTTAAAGCCATCTTTAAGGAAAATGGCAAAGACACTAAAGCTAAAAGTTAAAATTAACCGTTAAATTTTCCTGTAGTTTTAGTTTTCCAGTAGTTTTTAGTATTTGATTTAGTTCTTCTAACTACTTTAATTGAAATTTCCAAAAAAGCGCTGACAAAAATGAGCGTTCCTGACCGGTAGTTTTACCGTTTTACCCACGCTAATTGCATTTTTCTCGATTTTACCGCACAGTTCTGTTTTAGAACCAAGCCACTTAAGGTTCAATTCAACTAGCCAGCGTTACCAATCTGACAACTCCCACCTCTTAAATTTACACGGTGAAGCATTTAGTGATGCTGACTTGAATGCAGTGGCAGAGCATACTGACTGAGACGCTAACTTTGCTTTATTTCAGCAAAAGTTCAAGACAAAGCCAATTACGGTTAACCAGTTAGGTTTAGTCGATGTTTTTAACTTGTTGAGTGGTTGAAAACAGAGTTTGACTGGTGTACTTGAAACAATTCCCCAACTGCAAAAGGAAATAAAAGCTGCTAATGACATTTTCCCTGTCAATGAACAAAGTCAAATTCCCCGTGTAGAAGCACGCTTATTTGACTTTTTGGGCAGTGGCTTTTTCCCGGTATTGAACCCGAAAGGTTTAGGGTTAGCGGACAACGTTGCTAGTTTGTTTGAACAGTTTCAGCTAGCATCAATAAACTTAAGTGGTTTTGAAGTCAAACTGATTAAAACGCATGACATTGTCATTAAGAGTAAGGTACGTTACAGCTTTGAAGTGCAAATGCAGTTCCATGCTGTTTACCAAAATAACGACACTAAAGCTAATTTAGATTTTGCGCTCAATATTAGTACGATTAACTTCGCTACCTTACAAGAATTACAAAACAGTTTTGATCTTCAAGGTAGTGATTTAACGGCTGGGTTGTTTTACAAATACAGCGTTAATAAGTTAACTAGTGGTACTAACGATTTAACCACAATTGCTAAAACCGCTTTAGGTGAAAACATTATCCAAAAGCAAGTGAGCCTAACACAATCAATTATTAAGCCACGCCTAGAAGCTGCAAAAACTCAGTACAAACAGGATATTATTGCACCATTTGCAAAGGAACGTCAAGCTGCTTTGGCACAACATTTAAAAGAAATAGAAGAGGCCAAACAACGTGCCGAGCAATTATTAAAAGAACAGCAAGAAGCGGAAAAACGGCGCCAAGAAGAAGTTAAGAATGTCGCAGAAACACAACAGTTTAATGATAGCTTAACTTCAGCACAAAAATTTAAAGAGTACTGGTTAAAACAAGGTAAGGATGTTACCAAAAAGGTTGAACTCATTCAGGCGCTGAAATCATCTTTCTTCCGCAACCAAAACCGTACCTTTAACTTTTTAATTGCCGGTTTTCGAACTGCCATTGATTGGTACTACAATCAAGAGAAAAATAATACCACTGCTAAAAATAACGCTTTTGGTAAGAATGGCATTCAGTTCCCTGTGGCTGGCTTTCAGGGAATTTACATGTCACAGTGATTGCGTGACGAGCTCTCTGGTAAGACCGACATTAAGCTCAACTTAAAAAGTTTAAGTGTTCAGAATGAAAATAAAAATTCTTCCATTAATTGGAACAAGCAAAAAAGAATTGAGATTAAACAAGTAAAACCATTTAACTATTCTTTTGAAATTAACTTGAAGTACACTGGTAGCTATAATGTCAGTCTTTGATATCTAATTGGGGCTGCAATTGGTGGGATTCCAACATCTTGAAGTGGAACTATGGATATGAAGTTTATCGTTGATGGTGATTTGGATAGCGGTATTGTTACCAAGCAAGATTATCCCGGTTCTAAGTTTGAATTTACCGAAGACAAACTGTGGTTTACCCTGCACGTTAAACAACAAATTAAAGTTAAAGAGCAGGGCTTTATGAACTTGCTTAAGGGTCAAAGTTTGGACAACTTGGATCTACGCACTGGTACAACTAAACCGCCAGTAGTTGATTTAGCGAGCTACCTCCACTTTGTTATTTTGACAGCTAAGTAGTTCATTGAGCCGCAAAGGCAGCAAATGGAACAACTTTTAGACCAGTTTTTAAGTGGTGTTTTAAGCGAATGGAAGGCTTTTATTTTGCAAGAAGCCAAACAGACCTATTTTCAAGAATTGTTAGCTAAACTAAAAAATGTCGAGCAAGAGCTAACTCCCAAAGCCTCTCAAGTCTTTCGTCCCTTTAGCTTTTTTGCACCAAATAACACTAAACTAATTATTTACGGTCAGGATCCATATCCTAATCCACAACACGCTTGTGGTCTTTCGTTTGCCTCTAACGCCCCTAAACTACCACAAAGCTTAAAGCGGATGATTCTGCGCTTGCAGCAAGAGTATCCGGAACTAGCAGGTCAAAATCACTGAACGAAGCAACTGTTAGAGGGTTGAGCACAACAAGGCATCTTGTTGTTAAATGGCGTCTTTACCACCAATGCCTTTCAAACCAATGCGCACCGCAATTGGGGGTGAGAGCAGTTTAACTGTCATTTACTCGATTTTCTCTTGTCACAAAAACTCTATGTACTACTCGTTTTTTTGGGCAAACAAACCGAGAATTTTGTTCTTAAAAAGATTGGTGGAGCAAGCCAATTTGCTAGTTTATCCTACCCCCATCCCTCACCTTTAACCGGGCGGAAGTTTTTCGATCATCCCGATGCTTTGTTTAAGCAAATTAACCAGTGATTAAAGCACCATAACCATACTCCAATTGATTGGACTAACGGCCAGGTACAGTACCAGTTCTAACTGTTTTGGTAAAGTTTTGTCTTTAATTCAACAAACAAAGCTTTAGCATTTTTCATTATTTTTCTTATGATTTTTATTCCGCTTGTACTCAAACAGAAAAGCACAGCGAGCAGTCTTTTCTTTACAATGTAAAGAACTGCAAAGCTGCTTAAACCGTCTTTGCAAGCTTGTTTTAGAGTTGCAAAGCAAAGTCCAAAATGGATAAATTAATAAATAAACCTCAACCATTAACTTCTGATATTTCCACTTCTGGATTTATTTACTTTGCGGTTGTTTTTGTAGCCATTATGGGCTACCTTTTATTCAAAAATCTACTGTTTTTATTTTTCTTTAAACGCTATCCAAAAAACACTACCAAAATTGGGGTAGGTAACATTACCACGATTGCCATAATCATTGCTGTGGCGGTGTCGATTGTTTTAGTGTTAATGGCACTAGCAGGCGGTTTAGCCGCTGCTTTGTTCCGTGGTTATCCTGGTTTTCGGGTCACCTTGGAGTTAATCCTTGTAAAGATTTCGGGACTGTTGTTTGGTCCAATTGTCGGCATTTTTTCCGCGGCCACAATTGACTTTTTAACGGTCATCTTTTCGGGTGGGGTTTTTAACGTTGGTTATGTTTTAGGTGCCATCTTAACCGGGATGATTGCTGGCATACTGCGTGAAGTTTTAATTTCAACAGCACTTTTACACAACCGTAATTTATCGGATTTTGCCTACCTAGTGCTCTCGATTGGCATGGTCATTGCCGCCTTTTTAATTACCCAGTTCTTTGTGCTTGGGATAAGTAATAACCTTAAGGAGATTAAGGGTGATGAAGAGTTTCGCTTGAAATTTAACGCACCACCAATTGTCTTTGAGCTTTCGTTAACCCAGTACGCCAACATTCTGCTCTACTTTACCATTGCCATAGTTATTGCTATGCTGGTGTTGTACATTGTTTGGTTAGTCAAACAGCGTCACTTAAGTTTTGAGCACTCTCGTTTCTTTTACCGCAGTTACAAACATGCTAATCACCAGTTTACCCTGTTTGTACTAACTAAGGAAAACTGGTTCTATTTAATCTTGAATGTAATCACCTTAGCTTCCACTAGTTTGTTGATGATCAACATTGCCTTCATTCCGATCTTTGACACGCAAACAACTGGTCAAACATACGAGTTTTGACTATTAGCACGCTTACTGTTCGCTCCCGTAATCTTCTTGTTGGACATTATTGTGATCTATCCAATTCTGCTGTTATTAACCCCGCTAATGCTCAAAGGTTTTAAAACAGCAGTTTCTAAAAACCAACGCAAAACACTAAAACAAAGCTTTACTGATTTGCAAAGTGTAGTGTTGCCCATTATTAACAAACGTAAACACCAGCAGTTGCGCCAAGAAGAGTTAAAACGCTTAGCACGAGCCACTCACTTTGATTTGACAGAAGGCGAAATGGAAAAGCTCTTGGTCGAATTTAAAACTATTACCCAGAGCTTTGACCGGGTGATGAACATTGATACCACCAGTGTGGAACCAATGTACGCACCTTTCAATACATCACCAACGCCACTGCGTAAAGATAAGGTAATAGTGGAAAAGCATCCCGAAAAATTATTAGCCAACTGTAAGGAAATGAGTGTTGGCTTTGTCAAAGTATAGTTGTGAAATCGCAAATCTTAAAGCTCCAGCAAACACTAACTAAAAAACCAGCGTCAATCAATCCGTTATTACAACAAATTGATGGAGCAATTAACGAACACTGATCGAGTAACTTTTTGTTGAAAAATACGGTTGAGTGAGCACAAGCCCAAGCACCCAAAAACCGCAGTAAATCACCACTCAACAACATTCCCTTTGTTTTAAAGGACAACATTGCCACGAAGGGAATTGTTACTACTGGTGGTTCGAGGTTTTTGGAAGACTATATCCCGCCGTTTTCAGCAACTGTGTTTGAATTGCTAAACAACAGTGGCGCTTTATTAGTTGGTAAAGCGAACCTCGACGAGTTTGGTTTAGGCGGTACGGGGCTCCATTCGGGTTTTGGTTTTGTCCACCACCCTTGAAACGAAACCCTAATTCCTGGTGGTTCTTCATCCGGTTCCGCTTATGCAGTGGCGCGCGGCATTGTTCCCTTTTCAATTGGTACGGACACGGGAGATTCGGTACGCCGTCCAGCTAGCATTTGTAACATTGTTGGTTTTAAGCCCACCTATGGTTTGATATCGCGGAACGGAGTGTATCCTTACGCGCCTAGTTTAGACCATGTCGGGATCTTTGCGCGGTATGTGTATGATGTAGCACTTGTTAGTGATGAAATCATTAAACATGACAAAGCTGATTTTTCCGCTCAAAAATCTCCTGATGCTGGCAAGTTTACCAGGTCCTTAAAAGAGTCCTTCAACAAGCAAATCAAGATAGGTTATCTAAAACCGCTAGAGGAATGGTTTGACATTGAACTGAGCAAAAAGTGAAACAGCTTAAAGGAAAGAATTACCTTGGAGGGTTGTGAGTTAATTCCGTTTCACTTTCCGCTTGAACTGCTAGAGGTAATTGATCCAGTTTACAAGCTGATTAGTTACAGTGAAGCGGTGAGCTGTTACAGTAATCTCACTGGGATTGTCTTTGGTCAAAAATTGTTTGAACCCAACCAAGCGAGTGACTTTTCCAAAACAATTACTGCCAACCGCGACCGTTTCTTTGGTGAGCAGTTAAAACGCCGCTTTATTATTGGTGCTTTTGGTACTGACAAGAATAACTTTACCAAGTACTTTGAAAAGGCTCAAAAGATCCGCCGGGTAATGGTTGATGCTTACTTAAACCTGTTTAAAGAAGCGGATTTCATTGTGTCACCTAGTGCATCCGGCTTTACTAAAACAATAGCTGCGGTGCAAAAGGGTGAGAGCTTTACTAACCTCGTGGATGACTTTTTACAACTAGCTAACTTTGCTGGTAATCCTTCAATTACTATTCCCTGATTGGTCAAACAAAAGGACCAAACGATTGGTTTGAGTGTTAATGCTAACTGCTTTCACGACAAGCAATTATTACAAGTAGCTGCTTGATTGGAGGAATTATTCCAAATTGAACATGATGATTAACTTTGAAGCAATTATTGGGATTGAGGTACACGTGGTCTTAAACACTGCTACCAAAATGTTTTCCCCAGCGCCCAACCAAGCGCAAAACGCCACACCAAACCAGTTCATAAACGCGATTGATTTGGGCTTGCCTGGTACCATGCCCCAGGTCAATGAGCAGGCGGTACAAAAGGCGTTAATCCTTGCTGACGCGCTCAACATGCAACGGGTACAGCCAGTCTTAGTGTTTGACCGTAAGCACTACTTTTACCAAGATCTACCTAAGGGTTTTCAGATTACCCAGCAAAACTTTCCGATTGCGCAAAACGGTTACGTTGAAATTGTGGAAAACAACAAAGCGCAGCGGATTATCATTGAACGGTTTCACCTAGAGGAAGATACTGCCAAGCAACATTTTGTCGATGGGCAAATTCTGCTCGACTTTAACCGTTGTGGTGCACCGCTAATTGAAGTAGTTACCGCGCCCGTGATTAAGAGTGCTAAGCAGTCTAAGGCATACTTGCAAAAGCTACGTCAAATTTTGATTGTTAACAACATTTCCAACGCTAAGCTCGAAGATGGCTCGATGCGCAGTGACTGTAATGTATCAGTGCGCTTAAAGGGACAAACTGCTTTTGGCACCAAGGTCGAAATTAAGAACATTAACTCACTCAATAATGTGGAAAAGGCAATTGATTTGGAAATAGCACGTCAAGTCAAAGCACTAATTAAAGGCGAACCGGTTCAACAAGTTACCCTAACTTATGACGACAAAACCAATACCAATGTCTTCATGCGTAAAAAGGACAATTCAGTCGATTACCGTTATTTCATTGAACCCAACATTATGAGCAGTAACATTGATGAACTGCTACAAAAACCCAACAAGGCCTTTAACTTAACTGAGTTCTTTACCAAGCTCAAGGAAGCTGGGGTTAATGAGCAGCTAAATCAATTAGTAGTCGATGATCTGACTCTTTTTAATGCTTACACCAAGATTAACAGTGTCATTAACAGTCCCCAAGATACCATTCGCTGGTTGTGCATTGAGTTAATGGGACAACTCAACAAGCTACAAAAACCGCTCAAGGACAAAACGATTGATCACTTAATTATCCTAATCCAAATGGTACAGAAGGGCACCGTGAACCAAAAACAAGCAAAGCAGTTAATTGAGTTAATGTTAGACAATGGGCAAGACCCCCAATCGCTGGCTAAACTACACAACTTAGAACAGATAACTGATGAAAAACAACTAACGCAAATCATCCAACAAATCTTTAAGGAAAACGAGGGTGAAATCCTTAAGAACTTAGATCGAGTTGAACGAATTCAAAAGCTAATTATAGGTCAAGTTATGCAAAGAACCCACAATCGCGCCAATCCCCAGCAAGTTTTTATAATTGTTGAAAAGTTGCTTCATGATTTCAGCGAAAGAGCAAGCTAAAAAAGGTCAAATAATTTACCGTTACATTCTCCTTAAAATTCAAAGCTTTAAATGGCCTGCTGGCACCCGCATCTTCTCTGAACGCCAGCTCGAAATTCGTTTCGCTTCATCTAGAAGTCTCATTCGTACTATCCTCAATACCCTCTTAGGTAAAGATCTAATTCGCCACACTAAAGGTAATGCTGGTTACTTTGTCGCTAAGAATGCTGGTTTTAGTTTCTTTCATAAAACCCAGGACAACAAATTGCCCAAGTGAGCTAAGCTCTCAACCTTAATGAAGAACCACCTACGCGAAGTTGACCGTGATGTTTTCAGCTCAATTGACAGTGGCGTTGACTTTGGTAACTTTAAGGGTTTGGAAGCCAAGTTATTTGATGAAAACAAGAAGAATTTTTTAAACCTTAGCTTCTTTGGCAAGGATGATGTGCTCCAAATTTTTAGCGAGCAAAACCTCCAGGAACAATTCTTTAAGGACTTTGCTTACAACGGCATTGTGGTAGAGCGCAAAAGCTCCTTAATCTGTGTGGATGATGAGACTAAGAACTTACTGATCTACGACCTCTTTTATGATGACAACAATAAGTTCATAGTAGCAATGCGCTCTGAATATTTAAACCCCCGCATCAAAATTATTAATGCTTAAAGTAAAGTCAGATTTTTTAACTAAAGATCAGGTTATTTACGATGTAGCGATAGTGGGTGCTGGGCCAGCTGGCATAGCAGCGGGTATTTATGGCAAACGCGCTAACCTCAATCTAGCCATTATTGAAGGTAGTACTCCCGGCGGTAAGGTTGTCAAAACCAACATTGTCGAGAACTATCCGGGTTACAAGTCAATTACCGGACCTGACTTGGGGTTGGAAATGTACAACCACCTAATTGATTTAGAGCCCACCTTTTTCTATGCTAACCTAATTAAGTTAGATAAGGCAGCAGATACCTTTATCTTGTACCTCGATGACAAAACTGTGGTGTTTGCCAAAACCGTTATTTACGCTACGGGAATGTTGGAGCGTAAACTGGGCGTAGCCAAAGAAGACCACTTTTACGGTAAGGGAATTAGTTACTGTGCCATTTGTGATGGGTCCTTGTACAAAGACCAGGTGGTGGGTGTTGTTGGTGGGGGGAACTCTGCCATTCAGGAAGCGTTGTATTTAGCTGGCATGGCTAAAACGGTGCACTTAATCCACCGCCGTGAGGGCTTTCGTGCTGATGAAACGGCGTTAAATAAACTGAGAAACTTACCGAATGTTGTTTTTCACTTGAACTATACCGTAAAAGAATTGTTGGGTAACAATACCCTAAACGGCATAGTGCTTCAAAACACGCTCGATCATTCAACAAAACAGATAGATCTCAATTGTGTCTTTCCCTATATTGGTTTTGAAAGTATTACCAAACCGGTGGAACACCTCAACTTAAAGTTAGATCCCCAGGGTTTTTTAATTACCAATGAACAGATGGAAACAAGTTTAAAGGGTCTGTTTGCAGCAGGTGATTGTCGTTCTAAGCACTTCCGTCAAATTGGTACTGCCATTAATGACGGCATTATCGCGGTGTTAACGATCCGTGATGTCCTTTAGTGTTCAGATTAAGCACGAGTTAGCCACCAACCCTTTAATTAAAACAGAGTGATCCAGCTTTTTAGCTGGTTATTTTCAAAACGGGTTAAAACTACTCGCTACTGGTCAATGATCCTTTAAAACTAGCACTGCTACTTTAAAGGCGTTGTTTAGCAATGCACTCCAGTTTAGTTTTCAAATCCACGAAACGAGCACTCACTGTGAGTTTAGTTTTAGGGCTTCGCAAACAGAGGTGGATCAGTTGTTAGCCTTTGATGCAACTCAGAGCGATTTACCGCTACAGAAGGCCTATGTCATTGGTGCCTTTTTGAGTGGTGGTAGTGTGAGCGATTTACTCCATTCCAGTAACTTTCACCTCCAAATTAGCACTAACAACGAACTGCAAATAAAACAGTTAATGAAGCTGTTTCATCCTTTTAAACAAACCACTAAGCGCCACCAGCTTTTGGTCTATGTTAAAAGCTATCAGGCCATTTGTGACTTCTTTAAACTAGTCGAAGCTTTTGATGGTTATCTAGCCTTTGAAGAAAACCAATTGCAAAAAAATTTTGCCTTAGAACAAGTGCGTAAAAGTAACTTAGAAATTGCTAACCTAATGCGCACTTTAAAGACCAGCACAAGCACAGCGGAACAGCTAAAAATCCTCATAAACAGTGCTGACTTTAAAAAGCAATCGCTCAACTTTCAGCGCTTTTGTTTAGTCAAACTAGACCATCCTGACTGGTCATTAGAACAAATTGCCCAGTTTTTTGAAAGGAAATACAAAGTGCAAATTACCCGTTCGGGAATTCAACACCTCAACGCTAAGTTAAAAAAACTAAACCAAAATTAAAGCATTAGTTTAAAATTAACACACATTAAAGCATGGACGCAATTCAAATAGTAATGTTTGTAATGGCAATCCTTTGTTTAATCATCGGTTTATTGCTATCCAACCATGGTTCTACTGGCGGACTTGCTTCGCTTTCAGGTCAAGATTTGGAAATTTTTTCGCAAAACCAAAGACCGCGGAATTGTCAAAATTCTCCAAATTACGATGTTTATTTTAGTAGTGCTTTTTCTTATTTTAGGGTTAGTTTTTCATTTTGCGCTTTAAAATCTAACAGATGAAAGTATTAACTGATTTACAAAAGCGAATCTTCGCGATTGTGAAAAAAGAGAACGGCAAACCTATTCCTCCGGGAATAGTTGTCAGGATGATGGAGAATCAAGCTGGTTTTCCGGGAAAGCAACAGGTATACAGAGCAATTGATGACTTGTTAGAATGACACATTTTTCGCAAGTCGGGTGGTGCTACAAACCAGCTGTTAATTAACTATGAACTAGCTGATCCGGTATTGGATCAAAAGTTTCAGGGCATTCTCAATTTAGGTAACAAGAACACTGGTTTTGTCCGTCCCTTAGATGATGACAAAACGGTGTACTATATCCATTTTTCCAACCTAGCGGGAGCACTGGATGGTGATCTAGTTGAATTCTGTCCGCTCGACAAACCACAGGTAGGTGATAAGTTTGATGCTGCAGTGCTCAAAATTGTGAAGCGCTCCCGGGTGCTGTACGCTGGTAACTTTTTGATTGAGTACTCCGATTTTGGTCAGGAGTTTCGCATTGTTGCTGACAATCCTCGTTTTTACCTCACCCCGATTGTCAATAAGGCCTCAGTTCCAGCTGAACTGGAGAGCAATACTAAGGTCGCTTTTCAAATTGATGAGTATGATCCTGCAAATAACCTGTGTAAAGTCTCCATTCAGCAAATCTTGGGGAATAACGATGAACCGTTAATTAACCTCAAGGCGATTATGTTAGACCACTCGATTGTCTTTGAAAAGAATGATGTGGTCGAACAACAAGCAGCGAAGTTACAGTTTGATGAAAAAGAGCAGAGTAAGCCCTACCGCAAGGACTTAACCGAACTTGCCTTTGTCACGATTGACCCCGCCACTTCCAAGGACTTGGATGATGCGATTTACGTCAAGCGCACCGACAAAGGTTTTGTTTTGTATGTTGCCATTGCTGATGTGGCTTACTATGTGCAGCGCAACAGTGAACTTGACATTGAAGCCCGTCATAAAACAAGCTCAATTTACCTCCCGGGATACTATGTGGTACCGATGTTACCAGAACGTTTATCAAACGAGCTGTGTTCTTTAAACCCAAATGAAAAGCGGTATGTGGTGGTGTGTGAGTTGAACTTTGATCATGAAGCACGCTTAAACTTTAGTGAAGTGTATCCCGCTACCATTGTCTCCCAACGTCGCTTTGCTTACAGTGAAGTTAATGATTGACTGGAAGACAGTGATGCTCTAAAGGATGAAAGTGCAACAGTACTAGAGAGCTTAAAGGCTGGTTTTACCTTGAGCGAATTGATTGCTGAACAGCGCAAAAAGAAGGGGACTATTGACCTATCTCACAGTGAAACAGAAGTAGTCGTTGATCAAAACTACTACCCGATTGAGATCCGTTTTCTCACCCACGGCAAGGCGGAAACAATGATCGAAAACCTAATGGTGGTTGCCAATGAAGCGGTGGCTTGAACACTTACAAACCACAAAGTTCACTTACCTTACCGGGTGCATCCCCGACCAAGCAAAAAGAAGTTACAAATGTTGTTGGAAAACATTGTCGAGCTCAAAATTACCCAACCAAACTTTGTACTCGATACGGTCACTTCCACCCAAATTGCGGCTTGGTTAAAGGAAAATAAGGACAACCCGAGCTATGACATCTTTGTCATCTTGTTATTAAGAACGCTAGGTAAAGCCTTTTACATTGTTAATCCACTGATACACTTTAGCATTGGTTCACACCACTATACCCATTTCACTTCGCCCATCCGTCGCTATGCTGATTTAACGGTACACCGTTTGTTGTGGATGAATCTGTTTACTCCAGAGCGTTTTACTGACACTGAGCGTGAACAGCTCAACGCCGAGCTAGAACAAATTTGTGAAACCATTAACGATACTGAGATTAAGATTAATGGTTGTGAACGGACTGCCAACGATTACTTAACTACGCTGTATTTGTCTAAACAAGTGGGACAAACTTTCCACGGTTTTATTTCCGCTATTACTAGTTTTGGCATCTTTATGCGAATGGATGAAAACAACTTTGATGGACTTATTAAGATTACCTCCATTCCCGAAGACTTCTTTGTCTTTGAAAAGGATCGGATGGTGTTGCGCGGCAAACGCACCAACAAGGTTTTTAGAATTGGTGATAGGTTAACCGCGAAACTGACTGAAATTGATACAGTGCAGAAACGCGCCATTCTAACCCTGGTTTAATGATGACAGTGGAAGTCTTTTCGGTTATTATCCTTGTCCTTTTATTCTTAATCTTAGCGCTTACCTTGTTGTTTGTTTTACTCAACAAGCGTACTAGAAGCTTTGTTATTCGTACCTTTACTGGATTATTTAGATCAAAGCATACTACCAGTCAAAAGAACTTTTATGACAACTTGACCTCAACCTTACTGAGGTTATCAACTGATAAGATTGGTGCCATTATTGCCATTGAAAACCAGGACTCACTCGAGTCTTACGTCAATATTGGCTACCGCGTGACTTCGGACTTTTCCCCCGAGTTGTTAGTAACGATCTTTTACAACAAGCAGTCGCCACTTCACGATGGAGCGGTAATTGTGCGGGACTACCAGATTGTGAGTGTGTCCTCTTACTTCCCAATGACACGGCAACTAATTGATGTGTCATACGGTTCCAGACACCGCAGCGCGTTAGGTCTAACCGAAAAGTGTGATGCCATAGTCTTTATTGTTAGTGAAACAACAGGCAAAATTTCGGTGGCTGTGCGCGGCGTGATTAAAACACTGTCGAGTAACTCCGACCGTTTACAAGACCAGATTATTCACTATCTGACGGTAAAGCCGGGTTAATTACCTTCCACTCTTTATTTGTAAACAAAGGGTTTAGCGGATTAATGCGGTGGTAGTAAAACCGCCCTTGCAAATGATCGAATTCGTGTTGCAAGCACATCCCAAATAAACCAGTAGCAGTAATGGTGATCTCTTTTTGTTGGAGTCAATCAAAACCAGTGATCGTAATCCATTCGTGGCGAATAACATAACCCTGGTGCATTTTGGGAACACTCAAACAACCCTCACCGCTCTTTAAAAAGGATTTGTTGGCACTTAAGTTGATAATCTTGGGATTAATTAACAGACACTTGTGTTCCACCCCACCATCCATTAGGTGGATGTAGAACATTTGCTTTCAATAACCAATTTGGTTAGCGGCAATCCCAATGCCGGGAATAATGCCATACTTCTCAGCATCACCGTTGTAAGATGCATCAACGTAAGCCATCATTTTGGCAATGCAATCGAGACTCGCTTGGTCTAAGGGAAACTGAACTGGTTTAGTCGGTTCGTTAATTTCCTTAACATCGTCCAGCACTAGTCATGCTTTAGTTGGCAATAATTCCATATTAATTTGCGGTAAAATTAAAATTATTCTAAATATTGAAATGGTCGATACAGGGAGAATTTTTGTCATTACCGGACCGAGCGGTGTTGGCAAAAGTAGCCTTGTTAGATGTTTAATTGACCATTTCAAAGATAAACTGCGCTACAGCATTTCCGCTACCACCCGTAAAATGCGTAACAGCGAAACTGAGGGTGTGGATTACTTCTTTAAAGATAAAGCGGAGTTTGAAAAACTGATTGCTGCTGATGCCTTTGTGGAGTGAGCGATGTATAACGATAACTACTATGGTACCCTTAAATCCCAAGCTGAACAAATTATTCACAACGGTGGCAACTTGGTGTTAGAAATTGAATATCAAGGTGCTTTACAAGTTAAGCAAAAGTATCCCAATGATGTGGTGCTAATTTTTATTAAACCACCTTCAATGGAAGAGTTGTTAGTGCGCTTAAAGAAGCGTAATGACGAGGATGCAATAACAATTCAAAACCGATTGAAACAAGCTGAGAAGGAATGTCAACAAATTGGTCACTTTAAGTATGTGGTTACCAACAACGAGTTTGACAAGACCTTAGCGGAGTTACAAGCAATTTTACTAGCTGAATTTAATTAATGGACAGCACCAACCAAAACCTGTTCGCGTCATTGTCCAAAAAAGGACCCGTTCGCAAAGAAAATCAGGACTTTAGTGTTGTTACTTTCAACCGCTTTGGTCAGTTAATGAGCCTTGTTTGTGATGGTTTAGGCGGTTATAAGGGCGGTAAGATGGCTAGTGCCTTGGTGAGTGAAGTTTTTACCAAGTCCTTTACAGTCTTTGATTTTCATAGTCAAACTGAAAGGGCAGTGAAACAGTGGTTTGAAATTACTTTAATTGAAGCGCGGCGCACCTTAGAACAGTACTTTCAAACAATTAAACGTAACCAAGTCCAGTTTGCGCGAATGGCGACTACCTTAGTGTTGAGTATTATAAGTAAGCAAAACATTTGAACCTTTTGGGTGGGTGATTCACGTGCTTATTTAATTAACAGTTACCAAAGCTTGCAAATTACCGAAGACCATAACCTGTACAACCAACTGTTACAGATGCACGCTACCCCTGATGTGATTGCCTCTTATAAAGACAAGCTGTTAGCCCTCACTGCTACCGTTTCCAAAGATCAGGAACGCCAGTTGAAATACAGCTTTAGGTGTGATGTAGTAAACGCTTGGGACTTTTTACTGTTGTGCTCTGATGGTCTGTACAACTTCTTGGATCCCAACTGCTTTTATGAGGTCATTACTAGTGCACCTAACCTCAAAAAGGCCGTTACACAACTAGCAAAGTTGAGCTTGGATAACGCTAGTAATGACAACATTACGCTCAACTTGATTAATTTAAAACAATGGCACTAAATTTAAAGATTGGTGACATTGTCCAAAACAAGTACCGGATTGAAAAGCTAATCAACCGTGGCGGGATGAACTCTTACCTGTTTTTAGCGAGCAACCTCCACGTCCAGGAATTTGGTCCACTGCAAAAGCGCCAGTTTACCCGGTTGGTGTTAAAGGTAGTACAACGCACCGACAAGATTAACGACAACAACTGGAAGAAGTTTTTGGATGAAACCATTACCACGACACGGGTGTCACACAAAAATCTGGTGCAAACCTTTGATGTAGTTAGCCCCCGTTTAAGCGTTTTGAGTGAAAACCAGGTGATCGTTTTAGAGGACACGGTGATGATTGTAATGGAGTATGTTGATGGTCCTTCTTTGCGTGAAATGCTCAACCAGAAGGGTTACTTTAGTGTGCAGGAGGTGGTGTACTACTTTACAAAGCTAGTCAAAGTAATTAACTACCTCCACAGTTTTGAACACCAAATTATTCACCGTGATCTAAAGCCAGAAAACATCCTGTTTACGAGTAACTTGACTGACATTAAACTGCTTGACTTTGGCATTGCTTCCGCTGTTATTCGTAACGCTGAGAAAACTGAAGTGTTAACGGACGAAAACTCGTTGTTTGGTACAGTTAGTTATATGACACCCGAAGTGTTGGAAAGTACTGTCAACAAAGAAGGCAAACGGATTCGTAAACCCCCTACAGTTCAGTATGACATCTATTCATTAGGAGTTATTTTGTTTGAAATGTTAGTCGGACGCGTACCGTTCAATAAATCGATTGATCCTAAAAAAGAACGGGAAACCATCCAAAAGGCGCGTAACTTTGATGTACCTTTAATGGGAAACTTGCGCAGTGATGTTCCGGTTAGTTTGGAAAACATTGTCTTTAAATGTACGGCTGTTAAACGTGAAAACAGTAAGTGGATGTATAGTGACACGAAGCAACTCTTGGCTGATTTGGCGCAATGACAAACCGAGCAAACCTTAATTAAACCAGTGCACGAACGTATTCTAGAAGGACAAAACGAAATGCGTGAACTAATGGTAAGTAATTACCTACCGTGGTATTTAAGGAAGGGTGTTTTGATCTTTTTTTCTGTTGTTTTATTAGCACTCTTAATTGCTGTAGTTAGCTTTTTTATTATTACTGGGGTAGTTGTCCATTCGTAAAACTGGCATAGTATTTCAACGCTTTGGAAAGCAGTTTAAGGTGTTTGTAGAAAACCAAACACTAGTTGCTTTTGCACAGAAAAAACTACAGTGAAAGCGCGATTTTAAACTGTTAGTTGGGGACAAAGTTACCCTTGAGAATGGTGTCATTGTCGCAGTTGAAGAGCGTAAAAACACACTGGTGCGCCCAAAGGTAGTTAATGTAGATCAGGTAGTAATAGTCCAATCACTAATTGAACCCAAGATTAACTGACAACAGTTGTTTAAATTGTTAATCCATTTTCACGCTCAAAACGTGGCACGGTTAGTGTTAGTCATTACTAAAAACGACCTTGAGTTTGAACCTGCTGAAAAAGCCCGTTTAAGTGAACTAACCAGCTTTGGTTACCAACTCTTTTTTACAGCCCCTAATGCTGATTTACCTCTCACTTTATTTACAGAATTGCAAAACCGTTTTAGTGTATTCATGGGACAATCAGGGGTCGGTAAGTCTAGCCTTATTAACCGTTTGGACAGTCAAATTCACCAAGCGATCCAAGCGCTTTCAGCACACCAGTTCGGTAAAAACACCACCACTTCCACGGTGATGTTTCCCTTCCAAAACGGCTTTATTTGTGACACCCCTGGCTTTAATGTAATTGACTTTCCCAACCTCAAACAATTAGCAGCCCAACACTTTGTCGGTTTTGCTAGCTTAATTGGTCAATGCCATTTTTCCAACTGCACCCATCAAAGCGAAAAAGGTTGTTTTATTGTAAGTGCTGTAACCCAAAAATCGTATTCCTTGTGACTTTATGAGAGCTATCTAAAGTTGATTAATTAAAATCAACTAAAAAAAGGATTTATGGAAAGTAAATGGTTAACAGTTGACACTAAGCACCTCTATGGTTTTGATGAAGCGATTTTTATTCAGAAATACCAAAAAAAAGTTAACCAAATTCACCAACAGTTTTTAAATCAACAACTACCCGATGGTCACATGAATGGCTGGTATAGTCAACCCGATCAAGACCACAAGGGTTTGCTAAAACAAATTAACACAATTGCCAAACAGTTTAATGCTCTAAAAGTAACTGACATAGTGTATTTAGGTATTGGTGGTTCTTATACTGGCATTCGTGCCATCTTAGACTTTTTAAAACCAGAACAGAAGGCGAATATCAAAGTGCACTTTGTTCCTGACATATCGGCCTTTAACATTGCTGCAGTTGCTAAAGCAATTAAGGGTAAATCATGAGCTTTGGTAGTAACTTCTAAATCTGGTCGCACACTCGAGCCGGCTGTTACCTTTCGGTACTTCCGTAATTTATTGCACAAACAATACAAGCAAAAACATGCTTTACGCACAGTTGTGATTACCGATGCCGTTAAGGGTTTACTAGTTGGCATGAGTAACCAGTACGGTTATGCACACTTAACTATTCCAAGCAATATTGGTGGGCGTTTTTCAACTCTTTCACCCGCCGGTTTACTGTTAGCTAAACTTTGTGGTCATGATCCGAAACAGCTCTTATTGGGTACTTTGACAGCCAAACAAGAGTTAGCAAACAGTGATTTAAACACTAATTCGGCTTATTATTATGCTGCTTTGCGTCACTGACTTTACACGACAAAGAAGCTCAAAATAGAAGTGACGGTAGCATACCACAGTGCTTATGAGTACCTCTTGTTGCAACACCGCCAACTGTTCGGTGAATCAGAGGGTAAAGGTGGTAAGTCCCTGTTCCCTACCTTTTCGCTATTTACCACTGACTTACACTCAATGGGGCAGTTGTACCAAGAAGGGGAAAAGAACTTCTTTGAAACAGTGATACAAGTGCAAACACAATTTCACGACTTGGAACTACCACCATCAGACTTTAACAATGATGATCAGCTAGATTACTTATTGGCTAAAAGTATGAATGAAATTTCAAACACTGCCCTAGAAGCAGTTGTGGAAGCTCATTTTCAATCAAACGTTAACATCATTAAGTTAACGCTCAAAGAAAGAACAACCTTTATGTTTGGTTACTTTTACTTCTGGTTATCAATGGCCACAATGATGAGTGGTTCACTGCTGGGACACAACGTCTTTGATCAACCAGGGGTGGAAGTGTACAAGCAGTTAATGTTTGCTAAACTTGGTCGTGAATAGGGAAATTGCCTTTTCGCTTTTACCACTGTTACACCAGTTTGACCGCAAGTTGTTAGAACAATTCTTTGCGGACGGCTTGCGCTTAATCCACTATGACGTGATGGACCATTTTGTGGACAACACTGTCTTTCAAGGGGAACATTTAGATGAATTACAACAAATTGGTTTTCAGGTCAATGTACATTTGATGGTACAAGCATTAGAACAAATCTTGCCAGTATACCTCCACCATCAAGCCGTTAAGCGTATTTCCTTTCACGTTGAACCGTTTGATATCCCTACCATCAAACACTTTATTGCGCAAATTAAACAAGCGGGAAAACAAGTCGGTTTAGCGTTTAAATTCACCACTCCGCTTGTAAATTACGAGCGCTTGGTGCAACAACTGGACTTTGTTACCTTAATGAGTGTCCCCCCAGGAAAGGGCGGGCAAGCCTTTAACAGTGCTGTTTTTAATAACTTAAAACAAGCCCACAAATACCACTGCTCAATTGAAATCGATGGTGGGATCAAATTAGATAACATTCACCAAATCCAGGATGATGTCAATTTTATTGTCATGGGAAGTGGCTTTATAAAATTAGAACGATGGCAGCGCCAACAGCTGCTCAAAACCAATCAATAACTTTATATGTCTGCAACTGCTATTAGCGATCTCTTTGAAAAACCAGCCCAATTTAAAAACAAGAAAATTAAGCTAACGGGTTGATTGAAAAACAAACGTACTAGCGCCAACATTATCTTTTTGGAAGTTAATGATGGATCAACGCTGTTAAACCTACAAGCGGTGGTTAAACAAGACCAACCGGAGTTGTTTGCATTAGCTGAATCAATTAGTTTAGCGAGTGCTGTAAGTGTTTCTGGTACTGTAGCGTTAACACCCAAATCCAAACAACCCTTGGAATTAGTAGTCAAACAAATTAATGTATTGAGTACTGCGCGAGCAGACTATCCACTCCAAAAAAAGGAACACAGCTTGGAGTTTTTCCGCAACAACGCCTATTTGCGCGTACGAGCCCGTACCTACTTTGCCATCATGAAAGTGCGCAGTCTGTTGTCACAAGCCATTTTTGACTACTTCTTTAAGAATGACTTTGTTTTAGTGCACAGTCCAATTCTTACTAGCAACGACTGTGAGGGGGCAGGTGAGACCTTTGAATTAAAGCAAGGTAAGGAATTCTTTAATAAGACTACTTACCTTACTGTCAGTGGTCAGTTTGGTGCGGAGTGTTACGCCCAAGCCTTTAAAAAAGTGTTTACCTTCGGGCCGACTTTTCGCGCTGAAAAATCACACACTTCACGGCACCTTAGTGAGTTTTGGATGATTGAACCAGAAGTTGCTTTTGCCAACCTCAAGGACTTAATTAAGCTAATTGAAAGCACTGTCAAAACCGTGATTAAACAGGTTATGCAAAAAGCCAAGCAGGAACTCGACTTTTTAGAAAAGCAGTTTGATGTCAAATTAATGGAACGCTTAAAGCAAATTACAAGTACCAAAAACTTCCATGTTTTGGAATACATCAAGGCCTTAGAGATTTTAAAAACAGCCCAAGCTAGTGGTCAAGCTAACTTTGAGGTACAAGACTTTAACTTCGGTTTAGATCTCAAAACGGAACACGAACGCTTTTTGTGTGAACAGCATTTTCACAACCAACCGGTGTTTGTAATTAACTACCCCAAGGACCTTAAGGCCTTTTACATGAAGCAAAATGCTGATGGTAGAACTGTTGGTGCGGTTGACCTTTTATTTCCCCAAATTGGTGAGATCTGTGGTGGGAGTGAACGGGAAGGTAATCTAGAAAAACTAGTGGAACGTTGCCAAGCAATGCAAATTGATACCCAAACACTTAACTGGTATTTAGATATGCGTAAATGGGGTTACTTTGCCTCAGCTGGCTTTGGTTTAGGGTTTGATCGTTTACTAGCTTACATCTGTGGATTGGAAAACATCCGTGATGCGATCCCCTTTCCCCGTGCCCATGGTTCCATTAACTATTAAAACTTGACAGAAAAAGTTACCTAATTGGTTAACCATTTACCGCATTTTTATTGCTGTACCTACCATTATTTTTTTGGGATTAAATCACTTACTTGGTAGTGTTGCTAGTTTTACAGTGTTAGGCAATGTCACCATTCACCTACAGGTTAGCCTTTTTATTGGGGGAGTCTTGTTTATTACCGCAGTTATTTCTGATTATTTAGATGGGTATTGAGCGCGGAAGTGAAGGGTTGTGTCTAACTTCGGTAAGCTGTGAGATCCGTTAGCTGACAAGGTGATTATTAACGGTGTCTTAATTGTCCTAGTAGCATATGGCTACTTTCACTTTAGTTTTTTAATTGTGATTGTGCTGCGTGATTTAGTGCTAGACGGATTGCGCTTTTACGCTCAGGAAAAACAGCTAATTATTCCCGCCAACCAATGGGGTAAGTGAAAAACCACTTGACAAATGATAGCAATCTTAATGAGCTGTTTTGTGTTTAGTTTTTCTTTGAAGGAAACCAACAGTGCTAACACAAAAATCTTTTACTGAGCCATAGTGCATTTGCCCTATTATTTAGCTACAGCCTTCTCCTTGGTCTCGTTTGGTATTTACGCCCAACAAATATATAAAACCATTAAGGTTAAAGTAAAGTTATAATTTTGGAACTTTCTTATTGTTGTGTACGCGCGCTTAATTGCTGAAAAACTGCTAAATCATAAGCTCACCATTGCCACTGCAGAGTCAGTTACTGGGGGCCTTTTGAGTAGTAGCTTAACCGACATCGCTGGTGCTTCTCGTTTCTTTAAGGGTGCCATTGTGGCATACAGCAATGAATTGAAAAAATCACTTTTAAATGTCAAACAATCAACCTTAATTAACCACGGTGCTGTATCGCGTTATTGTGTTCGTGAAATGGCGTTAGGTTTAATGCAAAAATTCAATGTTGATATTGCCGTGGCCTGCAGTGGGTTAGCGGGTCCTGATGCATTGGAAAACCAAGCAGTTGGTAGCTTGTTTTTCTGTGTGATTGTTGCCAACAAGGCTTATGACTTTGAAACAAAACTACCAGCTGGCTCTAGAAACGAATTGCGCCAGCTTTTTGTGCAAAAAATCTTGCAAACAGTTGAACACATTTTAAGTGAAATTTCTTAGGTTAAATAAGGTTTAAGAGAACAAGATGCAGCGTACTTTAAATGTTGGGGTGATCCTGTGTGAAAGCTTTATTTCCAACGCCCAAAACCCAGTTAACTCCTACATTAAGATTTACGAAAACGTGAGGATGTTTGAGTTTGCGATTAAGCTACTTCAAGAATCGCGAATTAACTTCCAAAAGATCCTTTTATACGTTTTGGAAGAGCAAATTCCACTTGTGGAAAAAGTAGTTGCCAAGTACGAAAACTGCTGGGTCTTTCGTTCCAAGCACAACGAGGTTGAGGACATTTATGAAGCCAAGGGCTTTATTGAAGACAAGTACAAAATTGGTTTAAAAAGTTCTAAAAACCAAGCCTCTTCGTATTATGACAACTGTTGTTTTGTTGTCTTGGAAGCATGTCGACCTTTAACTTCCAAGAAGGTTGTTAAAAACGTGTATGAAAAGGCAATGATTGACGGTGCCGCTGTAGCTGTACTACCGTTTGAAAGGCAGCTTGTTTGTGGTGATAATACTAAGGCGGTGCGCCAGTTAAAGGACAAGGGCAACATGAACTATTGGCGTCAAAGTAGTACTTGGGAGTTGCAATTTCCCCAGGCTTATACGCTCAATAAGTTAAACCAGTATCATAAAAACCTCTTTATGAAGGCGCGGAACATGCTTGATTTAATGAACATTTCCGCTAAGAATCCCCTAAGCATTGTCGATGGTAGTGCTTACAGCTTTCGTGTAGTTACTAGCCTGGACTTTGAAATTTTGTTAGGTATTTTGCGCAATGGATAGAAAAATAGTTCAACTAATTCATAACTTTAGCGGTACAGAAAAACTAAAAGCAGCTGTTTTTTCACACGACGAACAGTGTTTCTTTGACTTTGTTAGCTTCGATGAATTAAACAGTAAGCTAACTGGGTTTCTGTTATTTGACAGCTTGGAGAAGCTGTTCAAACTAGTAGAAACAATTCAGCAAAAGCGCAGTTGGCTTTATGTTGATGAATTGTGACTGATCAATACTGCAACTGACAACCAACAACTCAATGAAGTGAGCGTTTGGTTAGTGAAAAAGGAGCTTGCGCAAGTCGGTGTTTTAACCCAATTGGATACTAGTTTAGTCAAGCTTTTAATGGCTTCCAAAAATACTGACAGTGCCCTTTACAACACTTACATTAAACCAGTGGAGTTGCAACAGTTTACCCAAACACCAGCTCCTGATAACGTTAATGCAGAGCAATCGCATTTAACCTTGGAGTCTACCACCGACTTGAACAACAGTCAACTAGCCAATACTCCAGCGTTGTGGGAAGTGGAACAAACGACCCAGGAATTGTTACCAACCATGGACTTTTCTAAGTTTATTGATGAATTAGATCAAATTACCAAAAACTTTTCTGATTTAGAGTTAGAACCACTTAGTTTTAACGAAGGTTTTGATGAGTGAAACCAAGAGTAAAGTACTCGTTTTAGGCGGTTTAGGTTACATTGGGAGCTGTTTTATTGACCAGCTTCTAAAACAGTACCCTGATGTAACTGTTAGTGTAATTGACATTAACCACACTAGTCTGGCGCTACAACTGTTACCAAGACAGGTCAACGTTCACTTTGTTAACTTACTTGACCGTGCACAGTTAACAGATACGATTGCTCAAATTAACCCTGATGTCGTGTTTCACTTTGCGGCAAAAACAAGCGTAAAGGAATCGACCGAACAACCACTTACATATTTTGACCATAACCTAGTCGGTACGCTCAACTTACTCCACGCTTTAAAAGAACTACAAAAACCGATCCAGCTGTTCTTCTCCTCCACTGCTGCAGTCTTTGGTAGTGCTAGTACACTTCCAATTCCAGAAAACTTAGTTTTAGAGGAAACACTCGCTTCCAATCCGTATGGCATTAGTAAGTTTTTAAGTGAAATAGTACTGCAAACACTTACCCGTAGCCCCCATTTTCAAGTAATAGCGTTGCGCTACTTTAACGTAGCGGGTGCTAGTAATCCCTTTGGTAACTTCAATAAAAACACGACCTTACTGATTCCTAACCTGATTAAAGCCTTTATGGAAAAGCGCACCTTCTTTCTGTATGGTGATGACTATGACACTAAAGATGGGAGCTGTATCCGTGATTACATCCATGTAGTAGACTTGTGTGATGCCCATTTGTTGGCGTGAAAGTGGTTGCAAGCCAATCCTAAAGTGCGCTTTGAAAGCTTTAACTTAGGTAGTGGTCAGGGCTTTTCCAATTGGGAGGTCATTAATACTGCTCAAGCAATCTTTGCGCCTGAACAATTACAGCTCAAAATTGAATCACGAAAGGCTGGTGATCCACCTGTTTTAGTGGTTGATTGCACGAAGGCAAAACGCTTACTCAACTTCCAACCAACCCGCTCATTACACAAAATGCTGAGCGATGAAACAATTTTTTATCGGGATTTTTATAATAGGCTTTAAATTCAATTTTGGCAATGTCAAAAATAGCCTTCAGGATGGAAAATATCTGCAAGAGTTTTGACAATGGTAGGGTCAAAGCTAATGTAGATGTTAACCTCACGGTTTACGAAAATACTGTCCACACATTGCTGGGTGAAAATGGTGCGGGCAAATCTACTTTAACTTCCATTTTGTTCGGTTTATACCAACCCGACAGCGGCAAAATTTTTATTGGTGAAGAGGAAGTACACTTCAAGTCCTCCAAGGATGCCGTGCAGCACAAAATTGGGATGGTGCACCAGCACTTTAAGCTGGTTGATAACTACACCGTCTTAGACAACATTATTTTGGGCAATGAAAGTAGTTTTTCCATTCCTTTTACCAACGGTAAGCTTAAACTACCTTTATTGCACCGTAAAGCTAGCGAAGCCAAAATTCAAGCAATGATGGAACGGTATGACCTCCATGTGAACTTGCACCAAAAGGTAAGCCGCTTAACGGTGGGTCAGCAACAACGGGTCGAGATCTTGAAGGTGTTATTCCGTGACAGTGACATTCTCATCTTTGATGAACCAACCGCTGTTTTGAGCGACCAGGAGATCAAGAGCTTTTTAAACATCATTAAAAACTTCAAGAAGATGGGTAAAACGATTGTGCTCATCTCCCACAAACTCAACGAAATTAAGGAAGTAGCGGAGACTGCTACCATTTTGCGTCAAGGTCACTCCGTTGGCACCTTCCAAATTAAGGACACTTCAATTGATGAAATGGCACGCTTAATGATGGGAAAGGAGTTGAAGAAAACCAAAAACAATACTCAGTTTACCGCCAAAGGTGAGCCCGTTTTAAAGGTCGAAAACCTCCATCTTTATTTAAACCAAAACTGGTTTTACAAGTTAATTGCGCGCTGGAACCAAAAGCGAATTAATCAACTCCAAAAACAGGGTAAGCCAGCGAAAACACTGTGGTTAAAGAGTTGACTAGAAGGGTTGGCAGCTATTGAAAAAACACCCCGTTTTATTCGTGGAATCGTTAATAATTTAGGTTTTGGTAGCCAACAGGTGTTTGATAAAGGGATTAGTTTTGAGATCCATAAGGGTGAAATTTTTGCCATTGCCGGGGTGGAAGGTAACGGTCAAAACCAATTAATAGATCTTATTTGTGGCTTGGAAAAAGCAGCACCAAAAAAGGTCTTCTTCAATGGTTTTGACATTTCACGTTATTCCATTCGCAAGCGGATTAACGCTGGTATTGGTTTTGTGTTAGAAGACCGCCATAAATACGGATTAATCTTAGACCAAACGGTGCGTTTTAACGCGGTGAATAACCAAATTGATCGTAAGCAATTTAGCAGTTGAAATTTTTTAAATCAAATGCAAATAGCTGTTTACACTAACCAAATCGTCGATAAATTTGATGTTCGTGGAGCGGTACAAGGTACAGCAATAGTGCGTTTACTTTCCGGGGGTAATCAGCAAAAGCTGATTATTGGTCGGGAACTGACAAAGCAGAACGAGCTGTTAGTGTTTGCTCAAGTAACCCGGGGTTTGGATGTTGGTGCCATTTCGTTTATCCACCAAAAGATTTTGGATGCAAAGAAACAAAATAATGCAATCTTGTTAGTGTCATATGAGTTAGATGAAATTTTAGCTATTGCCGATACCATTGGCGTAATTAACAAAGGCAACCTGTTGGAAGTCAATAAACGTGATGTAATGACGCGTGAACGCATAGGCAAACTAATCATGCAATAAGAAAAAACTGGGAATTATGAATGCTTTAAATTACCTCAAACACCGTTTTCTGTTTAGTAAAGACAAGTTTTGGTACGCCCCTTTTAAACAAAAGCAACGTCGGAGCATCTACTCCACCTTTTCGTTGATTGTTCTCTCCTTTATTGTTTCGTTCTTTTTAATAGTCGCCATTCCCGGCATTAAAGGCGGTACCTTCTTAGAAATCTTTACCCGTTTATTTAAAGATAGGGTTAACATCGAAAACTTTGCACGACAAATTGCGATTTACACCCTAGCTGCTTTGGCGTTTAGCTTTTGTATGAGTGTTGGGGTGTTTAACATTGGGATTTCGGGACAAATGATGGCTGGCGCTAATTTTGGTTTCATGATGATCCTCAAGGTTTTCCCCGAGTCATTCCGTCCTGCCTTTGGTGGTCAGATCATTACCATCCTCTTAATGATTCTAGGTAGTGTGACCGTTGCTATGGTGGTAGCTGCTTTAAAGGTCTTCTTTAAGGTTAATGAAGTGGTTAGTGCCATTATGCTCAACTGGGTAATTGTGCTGGTTAGCGCTTATCTAGTGGGTACTTACATTAAACCGGAAAAAACTGATACTTCCCAGTTTTACTCGATTGAACTACCGGATGCCTTTGCGCTGTATAACTTCTCTGATGTTCAACAAAAGTATGGTTGATTAACCTCGTTGGTTATTGCCATAGCAGCTGCCATATTCATTGCAGTTTTAATGAAGTTCACCGTTTTTGGACACAAACTCAAGTCCACGGGTTTAAGTGTGACGGGTTCGCAAGCCGCTGGTTACATTGTTAAGAAGTATCAGTTCCTCTCGTTTGTGATTTCAGGAATCCTTTCTGGTTTACTAGCAGCTGTAGTTTATACCGCATCCTTTGAAAAGCAGTTAACGTTCTCAGATGTGGGTGACTTTGGGATTACATCGGTACCGATTACTGGGTTTGATGGAATTGCAATTGGTTTAATTGCGCTCAACAGTCCAGCGCGAATTGTAATAGTTTCCACCATTATTTCATTTGTAACCATTGGTGCTAAACCAGCGGGGTTAAACGCTGCTACTGCGAGCTTGGTGTTGGGTATCATGATGTATTTCGCTGCCATTTATAACCTGATGATTTACATCAAGCCATGGCGCATGATTGTCAAGCTCAATATTGGTAAGATGAACGAAGCGGCATATGATGAATTCCAAAACGAGATGGCTGCTAATTTAGAAACGCTGTCGTTCCAGCGCTTTTTAGATAAACAAAAACGGAAGCATGATAAAGAGCGAATGGTTTGGTTTGACACCAAGCGCTTTGAGGAGTACCAGAAAAAGAAGCAAGCAACTCTACAAACCTTCCATGAAAATTCCTCGCAAAACTTACTGCAGTACTGAAAGCAACAGTTACTAGTAGCCGATGTAAAGCGATTAACTTTCAAATGAGACTTTCTTACCTTTAAACACCAGCAAAAGTACATCTTACGCTGGTACAAGGGCAAGAACAAAAAGCAAACAGCACTAGAAAATGAGTTTGCTAGTTTAAACGAAGCTATTAGTCAAAAATTGGAGGAGAAATAATGTCACAGTCTTTAATTTCTTTTAGTAACTTAGATAACTGGTTGTTTGTCGCTCCTGCACTGCTACTCGCTGTATTGAGTGGTTACTTATCTGAGCGGGTTGGTATTGTCAATATTGCCATTAACGGGGGCATGGTGTTTGGCGGGATGTTCTTATCCTTAATGTCTTATGCCTTTGTGCCCAATGCAAATGATTCTGCACCATCTTGATCACTAGCAATAAGCATCCCTTTAAGTGTTATCTTTGCCTCAGCTGTTGGTTTTCTGTTTGGCATTGCCGCCATTAAACTCAAAGCCGATCACGTGATTGTTGGCACAGGGGTTAACTTACTGGGCACGGGAATTAATTTCTTTGTGGCACAAAATGCCCGCTCATTGCTTAACGATACTGATTTAAGGGTACGATACAGCTTTGTAAGAACAGGAAACTCTGTAAGTATTGAGGGTATAGCAATCTTTGCCTTTGCAATTATCTTTGTTTTATTAGTGTGGTATTTGATGAACTTTACTAAAACGGGCTTGCGTTACCGTGCTGTTGGGGAAAATCCTAATGTGATTGATACCCAAGGTATTAGTGTGTATAAATACCAGTGGTTTGGGGTAATGGCTTCGACGATGGTAGCAGCTTTAGCGGGTTGTTGTTTTGCTTTAAGTCCACAGGTACCTAGCTTTTCTAGCGGGGATGTGAGCGGGTTTGGTTTTATTGCCATTGCCATTATGATCATTTCGATGTGACGAATTATCCCAAGTATTGTGATCTCGCCCTTGTTTGCACTAGCTTATGTGCTTACTACCGGTGTGGTAGGTAATGCTAATAACACTTACTTATTGAGAACTATTCCGTTTATTATTTCGCTGTTGGTAATGATGGTATTTGGATATCTCAATGTAGGGCCGAAAAACGTTGGTAAGCATTTCGATAAGGGTTTGCGATAGGTTAACCTTTATTTATAGTTATAGTCAAGCCAACAAATGAGTAAAAATCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACGTTGCAAAAATATTTACCGAATGACTTTGAAATAGTTTCTACCATTGGTCATATTCGGGAAATGGTGCACAAAAACTTTGGTTTTAACGAAGCCGATTACTCACCAGTTTGGGAAGACTGAACTAAATCAAAAAAGAAGTTTTCTAGCCTTAGCTTTAAAGGTAATCTTAAGGGCAAAAAACTTTTAAGTAAGTACGACATCATCAAAAGCATTAAAGAGAAGGCTAGTAAAGCCACTAACATTTACCTAGCAACCGACCCAGACCGTGAGGGAGAGGCAATTTCCTGACATGTTTACGATGTTTTGGATGAAAAGGACAAGTCGAAGTGCCAACGCATTACCTTTAACGAAATTACCAAGAACGCCGTTTTAGATGCACTGAAAAACCCACGGGAAATTGATCAGAGTTGGGTGCAGAGTCAGTTTGCGCGCCAAATTCTTGACCGGATGATTGGTTTTCGCCTTTCAAGGCTGTTAAACAATTACCTCTCAGCTAAGTCAGCGGGACGGGTACAATCAGTAGCACTGCGTTTTTTAGAAGAACGTGAACAGGAAATTCGTAGCTTTGTACCACGGTTTTGGTGAACGCTCGATGTGTTGTTAAATCCCAAGGCTGAAGGTGTTAGGGAGGCGTGTGCTAATCGTTCAATTCCAATTGTCTTGCGTGAAATTAATCCTGCTTTACGTGCTGGCTTGAAATTTGAAGAGGAGAAGAGCGTATCGGGAATTGACTTTTTAGATGAAGCATCAGCTAAAAAGTTTGGTGAACAGCTCAAGGGTGTCTTTGAGGTGTACAACATTGATGAAACCAAACACTACAGCAGTTCGCCCAATTCCGCTTACACCACCGCTTCATTGCAAAAGGATGCCATCAACAAACTAGGTTGGTCTTCCAAAAAGGTAACCTTAATTGCCCAACACCTGTATGAAGGGGTGAGTATTAATGGAGAACAAACCGCTTTAATTAGCTATCCCCGTACCGACTCAACTCGTTTATCAGCGCAATTTCAACAAAGTTGTAAAGAATACATCTTAAACCATTACGGAGAAAAGTACCTCTCTAATCGGATTGTAAGCGCTAAGGGAAAAAAAGGTGAAAAAATTATCCAAGACGCCCATGAAGCAATTCACCCAACTGACATGAACATTACCCCGGAAATGGTCAAGAATGCGATTAAGAAGGATGAGTTCTTACTGTACCGCTTAATCTGGATCCGTACGGTCGCTAGTTTAATGGCGGATTGTAAAAAATCACATACCCACATCCGGTTTATTAACGATGGTAATAAGTTTTACGCTTCTTCCAAGTCATTAGTATTTGATGGTTATCGCAAAATTTACGAACACTTTGAAAATAAGGAAAGTAATGATCTCTACATTGATTTGGATAAGATTAGGGTTGGTGATCGTTTCATGGCTAAAGACATTAAGATCACAGCCCGCCAAACCCATCCAGCAGCCCGTTATACCCAAGCTAGTTTAATTGAAGCGTTAGAAAAGTCCAATATTGGTCGACCTTCCACCTATAACACGATGGCTAGTGTTAATTTAGACCGGGGTTATGCTAGTTTAAATAAACATGCTTTTCACGTAACACAACTAGGGGAGCAGGTTAATGAAGAATTGTCCAATCACTTTGGTAAGATCATTAACAAAGAGTTCACTAAAAACATGGAGAAGTCTTTGGATGAAATTGCTGAAAATAAAAAGAACTACCAGGAGTTTCTCCGTGATTTTTGGTCAAACTTTAAGGAAGAGGTAAAGCTAGCGGAGGGTTCGATTCAAAGAGTGAAAAAGGAAAAGGAATTTGTTGGTCGTGATTGTCCAAGCTGTGCTAGTCCCTTGTTGTACCGCTATACTAAACGGGGTAATGAAAAGTTTGTTGGGTGCAGTAACTTTCCCAACTGTAAATACAACGAGTTTAGCCAAAATAAACCGAATTTAACTTTGGAAAAGCTCGAAGAACTGTGTCCGGAGTGTAACAGCCAACTAGTGAAAAGACGCACTAAGTTTAATCCCAATAAAACCTTTGTTGGTTGTAGTAATTTCCCTAGGTGCCGCTACATAAAGAAGGATAATGCTAGCTAGTTTAACGAGCAGAAGTGCCACTTCCTTCTCAATAATTTTAGCGTTGGTAAGTGCCATTTTGATCTTAAGTATCCTCATTTGGCTTATTATCACCATTTTCTTTGCTTGAAATTTACACCTCAAAAACAACAAGAAACGAACAAAGTACCACTTAGAGCCGGAACAAATTAAGCACAAGATCATCCAAAATAAGACTAAATTGGGCAAAATGTTGGACTTTTACCAACAGCAGATTAACACTACTGCAACTGAGCTGAAGTGGTTGGATGGTCAGTTTCAGCAAATTGATGAAACCGATAAAAAGAAAGCGCATCAAATTACAATTAGACTAGCGCGGAACCAACTGTTACAACAGCTTAGTGTAAAGTTAGACCAAAAGCAGTTTAGTCAACGCGCTAATAATGAACTGCAAAAGTTAAAGTTGTCTAACTTAGAGAGCTTTACTGACCAAAAGATTAAATGAGACCAGGAGGGGATGAAATCCGCTGTATCCCGCGTTACCATTAACGAGTGAACCTTTAACCATTTTGCTGGTAAAAACCGTGTTTACTGGGATTACTTTAAACAAGTTTGTGATGTGGATTGCTCGATTAAACCACTTAAAGATCAGTTAGAGATTACTTTTTCGAGCTGAAGCTTATTGAAAAGACTGCAAGCAAAAAACCTGTTTAATAAGTTAATTGCTCAAAGTAGCAGTGTCAAGATGAGCGAAAAACTGATTAACAATGCTTTGCAATTAGTTCAGGACAATTTGGCATTACAAGCGAGTGAAAGTGGCAATAAGCTGCTCAAGGAGTTTGAATTATCGTGCACTAACACACAGCTAGTCCAACTGTTGGGCTTTCAGCAGTTTTACTTCGGTACAAACCTGCTTAGCTTACTTGATTTGAGCCGTTCAATCGCTGTTTTAGTGCGCTTTTTAAATGAGCACTGTAAGTGAGAATTAAACGAACGCTTACTAGTGGAAACTGCTTTGTTTAACAACTTGCAATGGGTTAATAACAACGACTTCTTTTTAAAGTCACACAATGATTTAAAACAGCTGCACCTCTCCGCTGAACAGTTGGCGATTATTGAACAGCAAAACCGTCCCTTTTACATTGATGCTTATGCTTTATTGATTGCTGGCGTAAAGCAAATGCTGATGGAGCACGATGCTGTGGAACCAAAACAAATTCACTTTCATAATGCCAAAAAGGTAATGGAAAGCTTCCAGCTGTTTGGCATTGACCAGTTAGCGTTAATAGAGTACAACAACTGTCTCTATGGCTTTGTCACCACTAAACTCTATGAGATTAAACAATTAGATGATTTAGCTTTATTTAAAGTTTTGTTTAAAAGCTTTTTGAACAAGCATTTGAAACAAAAATTTGCAACAATCAGTTTATTTGTAAATACCCAAACACTCATGATCTAACACTTTAGATCATGGAAACAATTAGCGAACTTTTAGAATATAAAACAATTGAAAACAAATAGTTTATGGTAACTGAAATTAAAAGTCTCAAACAACTCGGAGAGTTATTTGCTAGCAACAATAAGGTAATTATTGATTTTTGAGCAGAATGGTGTGGGCCGTGCAAAATTACTGGACCAGAATTTGCTAAAGCTGCTAGTGAAGTGAGCACTGTAGCGTTTGCCAAGGTAAATGTCGATGAACAAACTGACATTGCCGCTGCTTACAAAATTACCTCACTACCTACAATTGTTTTATTTGAAAAAGGCCAGGAAAAGCACCGTGCTATTGGTTTTATGCCTAAAGCCAAGATCGTGCAACTTGTCAGCCAATAAAACTTCGTTTCATGATTGACTTGCTCGGGTTAGATCTTGATGGCACTTTGTTGTCTAGAACAAGGCAAATTAACGATCCTACTAAACAAGCTTTGGCTAATTTAATCCAAAAGAAGCCTAGCTTAAAGGTAATGATTCTAACCGGTAGATCATTATTCTCCACCTTGAAGTACGTTCAAGAGCTCAACGAGCTGTGCAAAAAACCGCTAGTGGAGTACTTTTGCTGTTATGGTGGGGCTAAGTTGTACCAACTCAATAACAACCAACCCCAAGAACAGTACAAGTTTTTAATTGACAGCCGGCAAGTTAAAACAGTGTTTGAAATTGTGGAACAACACAAGGGTTTGTTTTTGGCATACCTCGATAAACCAAAGGCACCCTACATTATTTTGGGGGCAAACCAGTTTTATGCTTGACTGATTAAACAGTTTTGGTACAAACAGCGTTGTGAGTACTTTAAAAATGATCATTTAACCGATGGCATCTTAAAGATTAATGTGTACTTCGCGTGTCCTTTAAGGTTGAAGAAGGTGTACCAGATCATTAAGCGTCAGTTTCAAGACACGCTTAACGTTGTGAGTTTTTCCAAGCACTTAATTGAAATTACCCACAAGGATGGAAATAAGGGCTATGCAATTGAAGCAATTGCCAAAAAACAAGGACTTTCGTTAAAACGGATGGCGGTGATTGGTGATTCCTTAAATGACCGTTCGATGTTTGAAAAGGTACAGTATTCGTTTGCAATGTCGAAATCACCCGATGAACTCAAACTGTTAGCCACTGAAATTGGCACTAAAACAAACCGCTTTCGTTTCAGTAGTTTAGTTGATTTAATTACCGAAAAAATTATTAACTAAAACTTTTTTACTTGATGTTTTGGCTTCCTAGTCCAAAGCCATCATAGAAAAGTGCTAGTGTTGTTCTGGCCGTTGCTTGGGCTTGATTTTTACCCGCCTGGAGGCAGTTAAAGACCTGTTCACGACTAATCTGTTCCCGTTTTCTCTGAATGTTAACTAGCAGATTAACGGTTGCTTCTGTAAGTGCGTTTTTGAGATCTAAATAAGATTTAGTACTAAAGTAAGCTTCTAGATCATTCCCTAACTGATTGGTTAAGGATTGATTGACAGGCTCTTTTAAAAGTGCCTTGAGAATTGTGAGCATATTGGTCACCCCTGGCTGCGTTTTGGAAGCAAAACGGATTTTGTTAAAGCTGTCAGTGGTAGCTTGACGAATTTTTTTAACCACTACTTCTTTTGGGTCATCCAAGTAAATTACTCCGTTTTGGTTCTTTGAGGACTTACTCATCTTTTTGGTTGGGTCAAATAAGTCCATAATACGGTTGGTATCTTTGTTTTGGACAAACTGGGGTAGACGCAGCTTGAGCTTAAACTTCTTTTGAATTCGCTGCGCTAAATCACGAGTAAGCTCTAGGTGTTGTTTTTGGTCATTACCAACGGGCACAATGTCAGGTTGGTACAGCAAGATATCTCCCGCCATTAGGGCTGGATAAGTTAACAGTCCGGTGGGAATGTTTAAGGTGCCGTTTGGGTTACGGGTTTGTTCCGCTTTTTTGGCCTTGAACTGCGTCATCCGTTGCAGTTCACCGAGATTGCTTTGCACCATCATTAAATAACCCATCATACTGTGCTCCAAAAGATCGCTTTGTAAAAAGAGACAAGCTTTTTGTGGATCTAGACCAACCGCTAATAAAGTACGTACCAAATCAAAGTTATTTTGTTTCAGTGCTTGGGGTTGAAAATCAACGGTAATTGAGTGTAAATCAGCGACAAATACAAACAGTTGGCATTGCTCTTGGAGTTCAATTAACGACTGCATTACCCCCAAATAGTTGCCTAAATGCTGTTTGCCCGATGCTTGAATCCCGGTAAGAGCGCGTTTCATCATATGATTGCAATTTGTAGAATCTATTACAGATAGACAAACGTTATTTTAAAAGAAAAATGCTTAAGAAAAAAGTTAATAATGATGCTGGTAAGACGTTTATCTTAATTTCATCATCTTGCTCGTCTTGCCAAAAGGCAATCGAATACTTTAAGGAAAATAATTTGAGTTATACGATCGAAAACTTTTACAAAAAACCGATAAGTGACAAGCGCTTTCGTGACATTTTAAGTTTGAGTGAAGATGGCACCGAAAGTCTGTTTTCCAAGCGTGCTGACCAAATTCGTAGTAACACTAATCAATCAGTGGAAGATTTGACCATTCCTGAATTGATTAAGTTAATTCGCGAACGTCCTTCAATTTTGCGTCGACCAATTATTATCCAGTACAACTCTTCGGGGATTCCCAAGCGGATGCGCATTGGTTATAACGCCGCTGAAATTAAGGTATTTGAACGCAGCTTAATGGAACCTAAAGTGCGTACAGTAAAGAAGTAATGAAGTACAAGATTTTTGCATCAACAACACCACAAACCGAGCCAGTTTTGCAAAAGTTAAAGCAGGTGTTGAAAGGTTGTGAGGCAGTTGAAAAGGGCTTTGATTATCTCTTTGTATTAGGTGGTGATGGTTTCTTTGTTTCTACTGTGGCCAACTATAACTGCCACAATTGTAGGGTAGTAGGGATTAACACTGGTCATCTAGGTTTTTACACATCGTTTAATGAAAAGGATTTGGATGATAATTTCCTCCAAAAGCTCCAGCAATGTCACTTTCAACGGATTAGTTTGTTGGAAGTTAGTGTCAATGGCCAGCAGCACTTGGTTTTGAATGAACTGGCAGTTTACACCAACACAGCTTACCCAATTAACATCTTTATTGATGGTGAAGCGTGGGAGTTTTACCGCGGTTCAGGTTTATTAATCGGTCCGCGTACTGGTTCGACTGCACTGGCTAAATCTGCTAAAGGGGCGGTGATTTTCCCTGGCATTGATGTGCTGCAAATTATTGAAATGAATCCACTTTTGCACCCCAATCAAGTTACTATCCAATCACCAATTATTTTGCCCAAAGAAACGCAAGTGGAATTTGTGGTCAAAAAAGCCTTTAACCCACAACAGTTTCCCACCTTTTACTGTGACGGGAGAAAGTTGGAATTGCCAAACGCTGACACAACTTTAGCGCTTAAACTAGTGCAATCCACACCGATGTTTAACATTTCACTCAAAACACAGGACTTTATCAATAAACTTAAGTCCACTTTTATTAAACAAAGCTAATGAAAGTATTGTTGTGAATTGGTTATGTGTTGAGCTTTGGCTTACTTTACCTTTATTTGGTAAAACGTGCTAAGAGGGCCGCGCTACAATTAAACAACAAACTAGTCGAATCACACACAATTCCTTTTGCTGTACGTGACTTTATTGCCGCTTGCGGGGGTAGAACTAACTTTGTTAGTTTACGCACCTCACCCACCCAATTAATTGTTAGTTTTGCCAAGCCAGAGTTAGTTCAAATTGCCGCTTTGCAAAAGCTTGGAATTAAAGGGATTAATAAGAGTCAGAACCAGTACCGCTTTGTATTGGGCAATTTTGTCAATCAATTAAAACAGCAAATTGAAAATGAGCGCTAAGTTAACGTTAGAAAGCATTGCAAAAACATTTGCCGAAACATCGATCTTTGCGATCTTGTTTTTAATTATTGTCATCCTCAATTTAGGTTTACTTGTTTTTTTAGCGTATCAGTACCGTGTTTACAAAAAGAAGCAAAGAGCAAACCTCACCAAGCAGACTTATAACAATGACTATGGCCAAATTGTTAATTTAAAACAGCAAAACGCTGCCAAGATAAAGGAGCTTGCTAACTTAAAAGATCAGTTAAGTGAATTAGGCCAAAAATTTAACACCACACTGAGTGAAATCATTAACAAACCACTTGTCAATGTGATTGATGAATACTTGGATGAACAGTTTAAACAAGCTGCTAATTTTCGTGAAGCAGAACTTAATGCCGTTTTAGATAGTAATGACCAGAAAACAGTGTTCCATAAGCGTTTGTTCAATAAATTTCACTTTGGTGTCGACAAGCTAGCTAATATTAATGTCAAAAATCCGCTCAATTTATGCTGGGTTGACAGCGCTAGTTTTACCGTGATTGAAAGTGATTTCCGCAAACTTAATGGTGTTGGCGGGATTAATAAAAAGTTGTTAATTGAAAAGTTAAGGATTGAAGATATTATCTTTACCAATATCGATAAGAAGTACTATGAGGTACAGATACTATCTGACTCGCCAGTAAAGGTACAAAAAACAGTGTTAACTATTCGCAACATCTTGATTAATGATTACGTGGACAATGAAAAAATTGAGTCATATGCTAGGGAAGCTAACGGTTACTTCAATGACCACTGTAAACTGATTGGTAAGCAGGTGTTAGAACGACTCAATATCTTTGAAATTAGCCCTAAACTACACAAGTTTTTTGGTTTATTAGCGTTTCGTTATTCTTTCGGTCAAAACGTTTTATCACACTGTCTGGAAACTGGTTTTCTCACCGCTTACCTAGCGCTTTTAGTTAACTTTAAACCTGATGTTGCATTAAAGTGCGGTTTGTATCATGATATTGGTAAAGCGGATGATGAAAACGGGAAGAAGAACCACACGGTTACAGGTGCCAAGATCGGTGATGAATTTTACTTTGAAAACGATGTCAAATACACAATTGCCAACCACCATAATAAAAATGTCGATAACGTATACTGTCGTTTAACTCAAATTGGCGATAAGCTTTCAGCAGGGCGTTTGGGTGCGCGCTCTGACAGTTCGGTTTTATTTAACCAACTCAAACAAGAATTAAAGCAAATTGTGGAAGAAACACTAGCACAATTTAAAACAACCATCCTTTTAGGACAAAGTGGACGCCGATTAGTAATTTGATTGGAAACCAACCAGCACAATAACATTATTGACAACCAACAGTTAACTGATTTGGCTACTACCATTAAAAGCAAAATCGTGCAAAACAACATTACTAATCGCTTTCCAATTAAGGTAGTATTACGCTATAACTTCGAACACAGCTTTGATACAAAGGACAAGAACTAGTTATGCAATACACCGTTTTAATTCCACTGTTTATTTTTATAGGAGCCATGGTGTTGTTTGGTTTTAGCTTTCAGAAAAAGCAACCACAACGCCGCATCGTTCAAATTCTGTTCTTAGCTTACTGTGTGGATTTTTTAGCACTAATTATTGCAGTGATGATGCTCACATTCTTAAGTTATGATGACTTAATGCTGGGCGTTTTAATTCCAGTATTAATCCTTTCAATCATTATGTTCTTTGTGATGATTATTGCCCACTATCCACTAATGAAACGCTTGTTTGGACATTAGACAAAACGTGTCTCATTCAAGTTGTGCACAAACTTAGGTGTTTTAAGTAGATAGTCGAAGCGTTAAAAAAGTATTTTGGGTCTTCTACTTTTTCCCTAAGTAGATGTTTTTTATTGATGTAAATAAAAGGCCAAATTTAACTCTTATGAGTTAAAAAGTTCATTTTTTAAATAACATTAAACCGGAAATTTCAATTAGTTTCCTCTTCTTCAAAAGGATTTTCCAAAAAAAGCAAAATAATGCAATTATTTTTTACTGTTAATGCGTAAAAAGAAGTTTTTGTCAAGGTTTTCTTTTAGTTCTCTATTTTTATTATGTGGAACATTGTTAAGTGCTTGCACTGGTATCCAAGCTGATCTTAGAAATCTTATTAAAGAAACCACTGGTAAAGACATTGATTTGTCTAAAGCAATTAAAACTAAAGAAGGCAAAAAGAACATAATAGCTTCTTTAAAAAATCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGAAAACAGAGTTTTGAAGAGGGTAAATTAGGTATTGCGGATTTTGATTTTGACCATGTAGCTTATCCTCAAACAAATGATCCTTTCACGATGGAGCGAAAGGTTGATCATTTTCAAATGACATACCAAAGTTTTAAGGATTTGTTAGTTGAGGCTAGGCTGAGCTATACCTTTAATTGATTTGGGGATTATTCTTCAGGTGATTTCACTGCTAAGAGAGGTGACAAGCATTATTTTTATCTTTTCCTTAAAATTAAATCTGATCCAAAAAAACAATTTTCAGCAAAAAAATTTCTTACTGAAGGAGAAGCGTTTACTGATCAAGAAGGAAAACAAACTACAAGAAATTTAGAGTGAATTGAGTTTAGCGCTTCGATTAGCTGATGAACAAAAGGCAAAGATGATGTTAGTCAAAAAAGTTTGAAGAAATTTTTTAGAAAGCTTTGCTACTAACACAGGCTACTCATCAGATATTAATCTCTTTAGTTATTTGGAATATTTAATTAAGTAATTTGTTGTGATTTTTTAGTTTTACTTGGAAAAAATTTAGCCACCTGCTTTTCAACTTGAACAAATGGTTTTTTATTTGTACCGCATGAATAAAAAACCAGCAACTAACTTTTACAGATTAATTGCTGGTATTCTTTATTTTGAATAATATCTACTTAGAATCTAGTTTATCTAGGCGAACATCCATTGAATCCAAGCGATTGCTCATCTTTTGCAACATTTGCAAGATTAACTGTAGAGTTTCTCCTTGAACTTTTTGCTCCGCTTGAAGTTCTTTAATTTGTTCCCCTTGAGTTTGAACAGTTTCGGTTAATTGTTTAATTTGCTCACCCTGAACCTTGATCTGTTCACCCTACGTTTTTTGCAGTTCAAACACAACATCAACTTTACCTTCAACGCGCTTCAATCGGCTTTAGTGGCGAAGTCTTTGAGCTTTTCGTCAAGCTATTGGTGAATTACAAATTCATATGTTTTTGACACCTTTTTACTTCCTGAATTAGGATCATTAGGACCTTTTGGAAATCCTGGTTATTTAGGGTTCTCAGGTTATTCTGGTTTTTCTAGAAATTTTGGCAACTTCGGAGACTTTGGAATATCCGGTGTATTCGGATTCTCAGGTTTATCCAGTTGATCTTCTATTATCGTAAAGTCTTTATCCTTAAAATAGTACTCTTAGGTAGCTTGTGCAAACCATTTCTTTGCTTGGTATAGACAATTTGAAAATTAACTCTTTCCTTTGTCCGGGAAAGGATATTAAATTCCTTTTAGGAAATAACTTTAGGCATTAATAAATTCCTCCTTTCTGTTCAAGGTTTAGAAAACTAAAACGTGCAACTTTGTTGCCAGCAAGGATCAACATCATCGTTGATCTAATAAATATATTGAGTAAAGGCATAATTTCCCTTCACTAAATAAATTGAACGCCTTATTTTGAAATGACAACAAATTGACAAATTTCTTTTTAATTAAAGAAAATTTTGGAAATTAAGAGCTAATGCTTAATTTAAAGTCGTTGAATTTAAGCCTTCTTTTTGTTTTTGAACTCAATCGAATAAATAGTTTTGGTAATAATCCAATTGCTTTTTACGCATTTCAATTTCTTTGGGAATACTTTCGGTAAGATCGTTACATTTTTTTCAAAAGCAAAGAGGATATCGGCAATCTTTTCTTGAATTTCTAGAGGTGGGAAAGTAAGTTCAATTTCTGCTAATTTACTTATTCCTAATTTAGGAATAGGGCAAGCATAATTAACAAACTTAGATGTTTGCAATCTGAGTGCATAAGCTAAAAATTTAGGATTAATTTCTTCGTTTTTTACTTTTAAAACGCCACAGTCTTGTCTTGCATTAAACTGTCCTTTTCTATAGAATACAGCACCAGCATAAGAACCTCCTGTAGTTCAAGTCACGTACTCGCCATCTCGGTCACAATCTTTAATTTTGCCAACTTCACCATCATTTAAGGTTGAAGCTGAATAAACCGGGTATTAACCGAGATTATCCTTAATATATTTTTTAGTAATTGCACGACCCCTCGAGATCTCGCAAATATCTTTGATTTTGTAAGTTTTGATTTGCACAACAGTAACAGTTTAATTTCTTTTAAAAGTCATTTTCAGCGACTGTTTTAGATTTTTCTTTGTCATCATTATTGCGCTCTTTCTGCATATTAAGTGCTTGTTGGCAAATTAGAATAATACCCAGTTATGAACCGTCCTACTCCCAATTTTGAAGCAATCGATAAGAAGATTAGTGCCTTTGTCACTAACCACGATAACTTATTGGATAAGTTACTCAAACAACAAACCGAATTGTTAACTAGTGAAATTACGACTAATTTTGAGGTAACCCAACAAATCCAGGAAGAAGTAGCCAAAAAGACGAAGCAACACTCTAAAAACTACAAGTGACTAGCCACTGTTGTTTTAGCGAACGGTGTTGTAAGTCTCTTTCTTTTAGGTGGGTTAATCTACCTCTTTAGTAAGTAGTTCCCAATTAGCTTCAATTGAGCGGTTGTTCGTCTTTTTAACGTTGTAACAAAAGCCTAAACCAGTTTCGTATTTTGGTACTACATGCATGTGGTAGTGAAATACTTCCTGCCCTGCCTTAGCCCCTTCATTGACAACATAGTTTAATCCAGCTGGTTGGAGCCGTTGTTGTAGCTTTAAAGCCATTTCTTTGGCGAGTAAACTAACCGCCTTCAAACTCTCATCATCAGTCGAGGAATAGTTAACGGCGTGTTTTTTGGGAATTACAAGCGTATGACCATCAGCAACTGGAAAGGCATCTAAAAAGGCAACAGCGTGTTCGTTTTCACCAACCTTAAAGGATTTCACATTCCCCTCTACAATTCCACAGAAGATACAGTTATTGTTTGCCATACTTTGTTTTTGCACCATAATAACGGTTATTTATAACAAAAAGCCCCCACAGGATTAAAAACATTGATGCAAAAATAGGGATTGATCAAAACCAAACATAGCTGTTATATTTGGTTTGGGAGAAGAAACCATAGACCGAGTACGAATCATGCCCGTTGTCCCATATCTTTAGAAAAGGTACTGTTTGTAAATAAAAGGCATAAAGGCTTGGGTACACCCCTGCCTTGAGTAAAAACTTACCCAATTCATGTAGCGGCTCGCGCATTTTGTAAGTTTTAAAGAAGATTCAGATAAAGCCAAAGAAGCTAGCGGGGTAAATCACGTGGAGAAACAGCTGCGCAAATGAAATTCAATCAGTTAAAGGATTACCACTAAAACTGTATGGCATTTCACTCGTAATAGTCGAAAACCAACCCTTAACGTGTTCTTCTACATTAACAAATCCAGTAACACGTGACAGTACATACAGGTTAAAAAAGATGACGGTAAAGACCAAGTAACCTGCAGCACTTAAAAACAACGCATTATTGTCAAACAGCTTACAGCGTCGTTTACAGAGGTACATTAAGACACAAACAGCGGCAATTGTGTTGCTCTGGTAGGTAAAGGTATCAAAGTTAGTAAACCAGATTAATTGTGGTTGGTTGTTATAAACCACACTGATAACAGCACTAGCTACCATTACCCCAAGGTTAAACAGGGAAAACGGCGCGAGAAAAAGTAAATAAAAGTCGCAATTATTGTTAAGATAGAAAAAACGGTAAGCGAGTCCTATTGTTTTTTTCATCGCAAGTTAGTTAACTAATACATGAGTTTTAAAAAGATTACCGAAATGATGCGCCAAGCTGAACGACAAAGCAAGCAAAAAGCGCTTGATTTCGAGCAAAAATTGTTTGAGTACAGCTATAAGAATGCTGCCATCAAGATTATCATTTTTGGTAATCTCACCATTAAGTCTATCACAATCGATCCCGCATTAATTGACCCAGAAGACAAGGTCACTTTAGAGGAGATGATTACAGAAGCTGTCAATGAGGCTGTGGGTGATGTTAAGGCCAAGTACGACCAGTTAATGGAAGAGGCTATGCCACAGATGCCTGGGCTTTTTTAAGCTGCAGTGCTAGCTTAACCATTTGTTTATCTTTAGTGAGCTGTATAAAGGTTGGAATGTTGACGAGAACTGTCAACAATACAATGGCGAAATAGCTCAACCCAAAGCTAACTCCAAAGATTAAATCAGCATTTTTTGGATCTAGCTTGAGGAACACCGCTACATTCTTGTACTCACCGCTCAGTCAATCTTCAGCCAAAATGCCGGTAGTATTACCGGTAATTTGTCCCTTAAAGATGTTAACCATCAAGGCCACATAACTGAGATATGCTAGCGCAAACACGTGGATGTAGAAGAACGAAATTAAGCTAAAGCCATCGTCTCATAAAAAGACAGCCAGACTCAACAAGAAACTCAAGAAGTGCATCATGAAGTAAACCTGGTTGTTTTCCAAACCAACAAAGAGAAATTTGACAATCTCCTCTTGTTTTAATGGAGTAAAAATTAACTCGCCAAATAAGGTTATTGCAGCACCGTAAAAACCAAAGATCGCCAACACACCCGCCACCTTTTTCTGGCGTAAAAAGATAAAGATCGGGCCGATTAAGGCAAAGAAGGGGCAGAGATCTAACAAAAAGATCCTGCTCAACCTTAGTGAATCTAAAGGAACATTTGCAACAGCATTCTTAGCCTTGGCTGCAGTGCCGTAAATATCGGGAAAAATATAGATAATTTGCCGCCCGAAAAAGACATAGAGAAAGAGAATAGCTGCAAAAACTTGTAAACAATAAAACTGGATTTTTAATTTGCGAATTAATGGGGTAAATCCCAAGATAACTAGCACCCCGGCTACTGTTAGGATGACTACAAGATAGTTAAGCGACTGCATTAATTGCTTAAAATTAAGATAATAATTTTTTAAATTTGTCTTCGATGAGCGATCGTTTAAATGATCAAGCACAAAATCGCTTGCAAAAACTCCTAAACCTCAAACAAACTGGCAACGATCCCTACCTCATTACTAAGATAGAAAACACCCACTCAGCGGCTAGTTTTCAAAAAGCATTTGCCAACCAATCTGACGCTGAGTTAAAGCAGTACCAAGTGATCTTAACCGGTCGGATTATTGCGCTCCGGCAGACCTTTATTATTATCCAAGACTTTAGTGGGAAAATGCAGTTGTACATTAATAAAAAACTGCCCCCGAACTGTTTGAATATTTCAATAATTACATTGATTTGGGTGATCAAATAGTAGCCACTGGCCATCCAATGATGACCAAAACAGGTGTTTTAACGCTGAACGTAGAGCGATTACAAATTGTCGCTAAATGCTTACAAACCCCACCTGAAAAATGACATGGGTTGACCGATCCAGAAGCGCGAGCACGAAAGCGTTACTTGGATTTGACCTACAACCGTGCGCAGGTCGAAGTCTTTTTGAAACGCACCCAGATTATTACAGCTATCCGTACCTTTTTAAATGAAGCGGGCTTTCTAGAAGTGGAAACACCCATTTTACAAGCCGTTTTAGGGGGTGCTAACGCCAAGCCCTTTAAAACACACTATAACGCCTTAAAGGGTGATTTTTACTTGCGGATTGCAAATGAAATAGCACTCAAAAAGCTCATTGTGGGCGGTTTGCCGAAAGTGTATGAAATGGGGCGGATGTTTCGTAACGAAGGGGTGGATACTACCCATAATCCGGAGTTTACGAGCATTGAAATGTATCAGGCTAACGCTGACTATGCGGTCATGATGGATTTAACCGAAAACCTCATTAAGTTTGTTTGCAAAACACTCAATCAGTGAAGTTTTAACTGGAACGGGCAAACACTTGATTTAGCCAAACCGTTCAAGAAAGTCAAAATGGTAGATTTAATCTGCCAAGTTACTGGGGTTAATTTTGATGATGTTAAGGATGATCAAACAGCCATTGCGCTTGCAAAGCAGCACAAAGTAGAGTTAAAAAAGCACGAGCAGAACAAACAACACATTATTAACCGTTTCTTTGAACAGTTCTGTGAGCATACCTTAATCCAACCCACCTTTGTCACACACTATCCCAAGGCCGTTTCCCCGCTAGCTAAACAAGATCCCCACAACCCCGAGTTTACTGAGCGCTTTGAACTTTTTATTAATACAAAAGAGTTAGCGAACGCTTACAGTGAGTTGAATGATCCCCTAGAACAACGTGCGCGCTTTGAACAACAGTTGCAGGAAAAACGCATGGGTAATGATGAAGCGAGTGAATTAGATGAATCCTTTTTGGATGCTTTGAGTTTTGGTCTCCCGCCAACAGGCGGCCTTGGGATTGGGGTTGATCGGTTAGTGATGTTATTGTGCGAGTGCAGTTCCATCCGTGACGTTGTTTTCTTTCCCCAACTACGTGAATTGAAATAAGTTTGATTTCATTGTTTTAGGTGCTGGCATTAGTGGCATAGTTTTATCACATGTTTTAGCACAGCATGGTAAAAGTGTTTTACTGTTAGAAAAACGCAACCAATTAGGTGGTAATTGTTATGACAAGTTGGATGAAACCACCGGGTTACTGTTCCACCAGTACGGTCCTCATATCTTTCATACTGATAATCAGAAAGTAATGGATTTTATCCAACCCTTTTTTGAACTAAACAATTACCAGCACCGTGTTGGCTTACAACTGGACAACAATTTGGATTTAACGCTTCCATTTGACTTTAGCCAAATGCGCAAGTTGTTGGATACAAAAACAGCTAGCAGTTTAATTAACTTCTTTCAACAGCACTTTCCAGCTGAAAAGCACTTAACACTGATGCAGTTACAAACAATTAACTTTGCTCCAGTCCAACAGCTGTACCAGTTTTTAAAAACAAAGGTGTACGGTCCTTACAGTGTGAAAATGTGGGGCATGCCATTAGAACAAATTGATCCTAGTGTATTGGGTCGAGTCAAAATTAGTTTAAGTGAAAACAGTAGCTATTTCCCTACAGCCACTATTCAAGGTCTACCTAAGGGTGGTTATACAAAGGCCTTTACAAAGATGGTGGATCATCCTCTGATTGATTTAAGGTTAAACTGTCCAGCTAACTTAATTAGCGTAAACAATAATCAACTCTTGTTTGCAAATCAACCAATTACCAAACCAGTGGTTTATTGTGGTTTAATTGATCAGCTGTTTGGCTATTGTTTTGGGCGATTGCAGTACCGCTCGCTCCACTTTGAATGAAAGCGTTATGCTGTAAAACAACACCAAGCTTACCCCGTAATGAACTGGCCACTCCACCCAACAATAACGCGCCAAGTGGAATACAAACAGTTAACGCAAGAGGGTTTGGAGTCTAACCAAACGATTGTTTCGTGTGAAACTCCTGGTGCTTTTAGGGAAGGCGATCCCCGCTTTATGGAGCCGTACTACCCGCTAAATGATGTAAGTAATAATGCCTTGTTTGCGCGTTATTTAAAGTTAGCTAACGCTATTCCTAACATACATTTGTTAGGTCGGTTGGCACTGTACCAGTACATTGATATGGATAGAGCAATTGCTCAAAGCTTAGCTAAAGCCGAACAACTATTACAGTAATGGAACAACAAAAAATTAGGAACTTTTCAATTATTGCCCACATTGACCATGGTAAGTCAACGCTGTCAGATCGCTTAATTGAACGCAGCATTGGCTTTGAAAAACGCTTACTGCAAGCGCAAATGTTGGACACCATGGCGATTGAGCGTGAACGGGGTATTACTATTAAACTCAACGCCGTGCAGCTCAAAATGGCCCAAGGCAACCAGCAGTACTTGTTTCACCTAGTTGATACTCCGGGACACGTTGACTTTACTTACGAGGTATCACGCTCTTTAGCAGCTTGTGAGGGTGTTTTACTGTTAGTTGATGCTACCCAAGGGATTCAAGCACAAACGATTTCCAACACTTACTTAGCATTGGAAAACAACCTCGAAATTATTCCGGTAATTAACAAGGTGGACATGGAGAGTGCTGATGTCGAGAAAACCAAACAAGCCTTTCACCAACTGCTAGGGGTGGATCCCAACACCATCCCCTTAGTGTCCGCTAAAACCGGGCTTGGTATTGACCGGTTAATCACTACCATTATTGAAAAAGTACCCCCTCCAAAGGGCGATGAAAGTAAACCACTCAAAGCCTTACTGTTTGACAGCTATTACGATCCTTATAAGGGGGTAGTGTGCTTTATTCGAATCTTTGAGGGGAGTTTAAAACTCAATGATAAGATCCGCTTTGCTAGAAGTAACTCGGTTTACCAAATTGTGGAATTGGGAATTAAAAACCCCTTTTTTGAAAAACAAGACGTGTTAAAAGCCGGGGAGATTGGTTGGTTTTCCGCTGGGATTAAAAAACTAAGGGATGTAACGGTTGGTGACACGATTGTTCACGCTGAGGACACGACCACTCCACCCTTACCGGGTTACAAAAAGGTGTTACCGATGATTTACTGTGGTCTGTACCCGATTGACAACAATGATTACCAAAACCTCAAAATGGCCATGGAAAAGATTATTTTGAGCGATGCGGCCTTGGAGTATGAATATGAAACATCACAAGCATTGGGCTTTGGGGTGCGTTGTGGTTTTTTGGGGTTGTTGCACATGGATGTAATTAAGGAGCGGTTGGAGCGCGAGTACAACCTTAAGTTAATTTCCGCTCCTCCTTCAGTGCGGTATAAGGTGTTGTTAACCAACGGGGAGGAATTGGAACTCGATAACCCTTCACTCTTACCAGAGCGCTCGCGAATTAAATCAATTAGCGAACCGTTTGTACGCGTTTACATTGATCTACCCGATCATTATTTAGGTACAGTAATTGATCTGTGTCAAAACTTCCGTGGGCAATACGAAAAGTTAGAGGAAATTGACATTGACCGCAAACGCTTGGTCTACTTAATGCCCTTGGGTGAAATTATCTACAGTTTCTTTGATAAGTTGAAATCAATCACTAAGGGTTATGCTTCGCTCAATTACGAGTTTGACCAGTACCAGGTGTCCCAATTGGCTAAGGTGGAAATTATGTTAAACAAACAGAAGGTGGACGCTTTATCTTTTATTGCCCACCATGACTTTGCCTTTCAACGGGCCAAGAAGTTCTGTGTCAAATTAAAGGAACTAATTCCCAAGCACCTGTTTGAAATTCCAATTCAAGCCACAATTGGAAGTAAGGTAATTGCTAGAGAAACGATTAAAGCAGTGCGTAAAGATGTAACAGCAAAGCTGTATGGCGGGGATGTCACGCGGAAAAAGAAGCTGTTGGAAAAACAAAAGGAAGGGAAGAAAAGACTGAAAGCAATTGGTAGTGTGGAGCTCCCCCAAGAGCTGTTTAGTCATTTGCTCAAAGATGAAGATTAACTTATTTATAATTGATTACTGTTTAATTACTGATACCTTGTAAAGGTATTAACTAATTATTTTGATCTTTTATAAGTAATGATTAAGGATTTTGATCCTAGTGAGTTTGTCGGTAAAACTCCTACCAAGATTTTTGCCTTTGGTGGTATCCAAGAAGTTGGAAAAAACATGTATGGAATCGAGTACGATGATGAGATTATCATCATCGACTGCGGTATTAAGTTTGCTAGTGATGATCTACTAGGGATTGATGGTATTATCCCGAGCTTCGAATACCTCATTGAAAACCAAGCTAAGGTTAAAGCACTGTTTATCACCCACGGCCATGAAGACCACATTGGTGGGGTGCCTTACTTGTTAAAACAAGTCGATGTACCGGTGATTTACGCACCCCGGATTGCCGCTTCTTTGATCTTAAAGAAGGTCAACGAACACAAGGATGCTAAGCTCAATAAAGTGGTCGTATATGATGACTTTAGCAACTTTGAAACCAAGCACTTCAAGATTGACTTTTACCGCGTCAACCACTCGATTCCTGACGCCTTTGGTGTCTGTGTTCAAACTCCCAACGGTAACATTGTTGAGAGTGGTGACTTTCGCTTTGACTTTGCCGCTGGCGGGGAAATGCTCGATGTGCATAAGGTCGTGAAGATTGCCGAGCGCAATGTCCATGTCTTTATGTGTGAGACTACTAACGCCGAAATCCCGGGGTTCTCCCAGAGCGAAAAGTTAATTTACCGCAACATCAACAAGATTATTAAAGAAGCACGTGGTCGGGTCATTCTCACTACCTTTGCTTCCAACATTACCCGGATTAACGAAATTATTGAGATTGCTGTTAACAATAAACGGAAGGTCTGCTTGTTGGGTAAGTCGATGGATGTGAACGTCAACATCTCGCGTAAGATTGGCTTAATGGACATTGACAGCAATGACATTGTGGAAGTCCGTGACATTAAGAATTACCCCGACCGCAGTATCTTAATCTTGTGTACGGGTTCCCAAGGTGAAGACTCAGCAGCCTTAAACACAATGGCACGGGGTAAACATAACTGGGTTAGCTTAAAGTCCACTGACACCATTATTATGTCCTCCAACCCGATTCCGGGGAACTATGCAGCGGTGGAAAATCTCTTGAATGAGCTTTCGAAGTACGGGGTGACCATCTTTGAAAACTCACCGAACATGAAGCTACACGCCTCTGGTCACGCTACCCAGCAAGAGTTGCAGTTAATGTTAAACCTCGTGTTTCCAAGGTACTTAATTCCGATTCACGGGGAGTACAAGATGATGCGCACCATCAAAAACATAGCGCAAGAATGTGGCATTAACGGTGATGATGTTGGTTTGCTAGCTAACGGTCAGGTGATGTACCTAATTGATGGCAAGCTGTATTACTCAGGGGAAGTGATTAATGCTGACCCGATTTACATTGAAAGCCGCAATTCTTCTCCAGACTTAGCGCGGGTAATTAAACAACGCCAAATCTTGAGTCGGGAGGGAATGTTTGCTGTGATTGTAGTCTTTGACAAAAACAATAACATTTTAGGGATGCCTACTCTGATTACTCGGGGTTGTTTCTTTGCTCTAGATTCCAGTCCGTTAATGACTAAGATTACCCACTCAATTAAACGCGGTTTGGAAAACGTTATTCAAAACAAGCGTTTTAATACCCGTGAACAGATGATCAAGGAATTGAAACGAGTATGTAAGGAAACGGTGTCTTACTTCATTTGGAAGAACAAGAGTCGTAACCCGTTGATTTCAACCGTATTGTCTTGAGTTTAAACCTTAGTTTTAACTAATTCTCAATTGGACCCAATTATTGGAAATTAACACCTTTATAAGGCTGTTTTTGCCAGTAGTTGGGTTTTCTTTTGTTTGCTCTTGAGATCTAATTAATGCATACAAAAAGCCATTAATTGCCCAAAAATCAGATTCTTTTTACTTTCTTAAAAATATTTTTTTAAGATTTGCCATCTTTTTTCGCTTGGGAAGATGAAATTTAAGTATGGAACGGTTGTTTTGGGCAGTTTTTTAGGGCTTTCTGTGGTGTTAGCTGCCTGTGGTGCTCGTGGTAAATTTGACCAATTTGATGATGGCAAAATTAAGCTAGCATCATCCTTAACTTCCAAAGCAGCTGCCAATGCTTTACAAACAATAATAGAGAAGTACAACATTGTCAAAAGCGGCAAGGATTACCCCATTGAAATTACCCAAATTGCTGGTGGTTATGACGGCGGGCGGACTGATCTCCAAACCCGAGTTAGTGTTAAGGATAAAACGAACTTTTATAACCTTATTTTGAACTATCCAGATTTAGTTTCTGTGTTAGCGCGCAGTGGAATGGAACTGCTTTTTGACAAAGTTAATGTTGATAAATTAGAGCCGAACTTCTTAAAGTTTAATGAACAGATTAGTGGAGTCGCTAAAAGTGGTAACTATGGTATTCCCGTGTCCCTTTCAACTGACATTTTGGTCTTAAATGGTCCGGTGTTGCACTATATTTTGAACAGTGCGAAGAAAGAAGAGGCTAATTCTCAAGTTAAAAGTCAAATAAAAACATCACAAATTCAAGCTAAAGGTTCATTAACAATTGATACAGATGAAAATACCAAATCACTTTGAGAGAAAATTCAAAATTCAGCCAAAGCTAATGGTGAAACTAATCAAAAAGGACGAAAAGCTGCAAAATCAAATAAAACTGCATTAGTTCAACTTAAAAATGGCGCTGATACTACCACTAACGAAGAGAATAAAGATACCAAAACTAGTGATGATAAAGTTAAACAAACTTGAGGTGATTATGTAGAAAAGGATGATGGTCTTAAAAACTATACCTTTAGGGCTAGCGTGTTTGAAAACTGGCATGATTTCCTCGATTTTTCGACAAGGGTAGCTAAATCTTTTACTGAAAAGGTAAGCAACATTACCAATAAAAAGGGCACTGATATTCAGGGAGTGCTTGGTGTCGACAGTTCTCCTAATGTTTTATTTGCCTCTGTATTTGCTGCAGGTGGTGGTAATTATGAAAACTTCTTTTATAAGGTGAAAGATGGTCGGGCTGATTTTAGTAACTTTAAGAACAAGGGTAGTTCTTACCAAAACCTCCAAAGCGTTTTTAAAGACTAAAGTGAACTAATTAAAAGTAACGGTCTTTTTGTCAATAAAGGTGGTTCTTATTCTTCCAATTTCCAGAAGTTTCACCAATTAGCTTACAGTATTTCTTCCACTTCTGGCTTTAAGTATTCGTTTGCCGATAAGAACGCTAAGCGCTTGAAGTTTAGTGACGACAACACCTTCGTTGAATATCCTGATTTTACTCAGGAAATTAAGTCGCCAGACCAACAAAATGGTCAAAATACTGATGACAATAACAGTAAAAAAGAAGAAAACCTTTTAGGTACCTTTGAAGTTAAAGATTACTCGAAATCTAATAAAGGCGGGAAAGGAAAAAGTAAGGGTAAAGGCGGAAGCAGCAGTAATATGAAGACTATTTACATTTACAAAGGCTATATTCCTGAAGATAAAAAGAAAGGTGATAATGCCATTTTAGTTACTGAGAAAAAAATTATTGATGCTTTAGAAAAAGCTGCTAATTCTAATGGTGAAAGTACACAATCTAATCCAAAAACCATAGCACTTCAAGCAGCTAAAAAAGAAACTAAAAAGAGCAATAAGAAAGAATCAAAAACGATTGGATATACAACTACTGGTTATGTCAATGCTGATGGTAAACATATTTTTGATACAGACAGAATTAACAATGAGAGGTTTGATCGTAAAATTATTATTGGTGCTACTCTAGAAACTTTAGACCAATCTAGTACACTCCAAAGTGGTGAAGCAATTGTCTTACCAGCACCTGGTAAATATAAGAGTAGTGATCAAAAAACGGTTATGACAGCGCAAGGCCCCAAGATAATCGGGATCCATGCTAATGCTAAAGAGAACGAAGAAACACAAAAATTTGTTAATTGGTTTTAAATACGCCAGAAACTTGAGAAAGTAAGAGGAATAAGAAAAACTCTAATGAAAAACAAACTGCAGCACAATACTTTGCTCAAGAGGCATCTTACATCTTGCCCTTAAAAGAAAACTTTGAAAAGGTTCAAATTAACGATAAAGGTGAGAAAAATGAAAATGACACAAAGGGCAATACTTATACCGCTAAAGCTTTAGAACTCTTTAAAAAAGTTTCTCAAAATGAAATCGTGAGTTACAGTGACCCAAGTGACTTTAGAAGTGGTAAGTTTCGTGATGCGATTGGCTCTAACTTTAACGCCATTATTAATTCCAAAGGAGACTTCAATAAATTTATTAGTAGTTTTACCGCAAGTTTAGGCTCAGACTTTTAGTCTTTTAAATACCCAATTGATTTATTTTTTAATCGATTAATTTATTTAATGGAAAAACAAGTAGATAAATTAGTGAAAACTTTAATTCATCAAAAAGCGTTTCAATAAATCCGGTTCTTAGAAATAAGGTTTTAATAAAAAACATACCAGCAGTTAATGCTTCCAAATTAGCTGTGGGTATGTTTATTCTTGTCAAATAAATAAAAAAGCCATCTATCTAAAAGACAGACAGCTAAAAAATACTTTTTTTAACGATATTGAAATTAACAGAAAGATCTGTTAGCAAATGTGAAGGAAGAGGCTCTATGCAAACTTTCAATATGTGGCGTGTTGAAAAACCTTTAAGAATATGCACCAATTGGGGGGCAGAAAAGCAGGTCTTCAACATAAATTTTCGTTTTCCTGCTCCCATTAGATAAATTGAGTTGCTTTTTAAAAGACGCCAAAAAGATTAAATGTTCTTCTAATTTGGTTTTTTAACTTCTTCTAACTATTAATAGGGTCTTTTACTTTCTAAAAATTTTTTGAAGTTATTTTGTCACATTTACTTTTTGGGCAATTTAGATCATGAATACCGAAAAAGTATAAGATTCATTAAGTTAACGAGGTATCACCTCTTTTTAAAACAAGCCCAATCGCAACTCGTTTCTTTGTTTAACCAAAACTTTTTGCAAGTTTTGTTGGCATTTCTAAATAAATCTTTCAATTTATTTAGTGATGACACAAAAATTAATATGTTGAACTTTTTAAATAAATCAACAATGGTATTGATCCCCGTTGGCAGCAGTGCTCCAAGTTCTAGTTCCTTTTTAAATCACCTCAAGAAAGAAGGTATTGCTTAATGAAAGAGAAAATTTCTAAAAAGGAATACAATGCCTTAATCCGTAAAACTGGCGAAAAGCACTTTGACGGCGAAAAGGAAGAGTACGGTGATGGCACCGTTGGACTTTGAACTTATGAATTGCGAAAATATAAACTTAAACCACCTGTGAAAGTTAAATACGTAACGCAAGAAGAGTTTGGCGAATTCAAAGATGCCACCAACCAGCGGCTCACAAAGATTGAGAACGCTTTGGTCGCTCAGGGCGAACAAATTAGAGCTCAGGGTGAGCAATTAAGTCAACTAATTAAAGTTGTGCTTCTTCAGGGCAAACAAATAAAATCTCAAGGTGAGCAAATAAAATCTTAGGGCGAACAAATCAACAAATTAACTCAAACTGTTGAAAAGCAGGGCGAGAAAATTGAAGTCCAAGGACAACAGATTCAAACAGTTAATGAAACTTTGAAGTTTGTAGTTGAAAGCCTTGAAACTATCCATAAACGGCTCGATGTAATGGAAATTCGTTTAGACAAACTCGAGTCCAAATATATCTGATTCAAATAAGAGTACCAGCAGTTAGTTCTAAAAGCAAGCTGTTGGTATTTTTATTGATCTAAAACTACCTAAAAAAATTTTTTGCAATTTTGTTGTCACATATAAAGTCTTAGTTCAATTTATTTAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGTAAAGAGTCTGATCCCTGTTGACAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAGGAGGATTTAAACATGAAAAAAGTTAAAGTTCTAGAGAAGCCATCTGTTGAAAGAATGATAGAAGTTTTAAGCGACTCTGAAATATTGAAGTTTGATTTTTCTTGCTATTATTTACCTGATGGTACTAATGAGACGCTCATAAGAATCTTTTTTCAAGAAAAAGTTTCAAGTTCTGTAGTAACAAAAGATAAAAATTCTTTAAGACTTGATGAATACTTAAAAGACTTTATTAGCTTCTTAAAAACACAAAGAAAAAACTAATAGCGTAAAAATCCAATAGTGAAAATTCACTGTTGGTATTTTTATTTATGTCAAATAGAAGATATTAGATGGAAAATTCAAATAGAAACTATAACACACAGAGCTAAGTAATTTTTATTTGTACTTTTTCTTGTTTAAAAAGCAGTTACTATTTATTCTTTCTTTATTTCATTTTTATCTTTGAAAAATGAGAAAGTTGACAATATTAAAAACTTAAACTTTTTTTAAGATTCTGTAGATTTTCTGGGTTTTAGTGCATGAAATTGAAATATGGAACCATTATTTTCAGTGGTCTTTTGGGAGTTTCCGCCATTTTAGCTGCCTGTGGTGCACGCGGCAAATTTGACCAAATTGATGATGGCAAAATTGTTTTAGCTTCTTCTCTTACATCTAAGGGTGCAGCTAATGCCTTGCAAACAATTGTTAAAAAGTACAACGAAGTTAAAAATATTGATGACTATCCCATTGAAATTATCCAAATTGCCGGTGGTTATGATGGCGGTCGTGGTAACTTACAAACAAAACTAAGTGTTAAAGACAAAAATAGTTTCTATAACTTAATTTTGAACTACCCAGACGTAGTTTCTGTTTTAGGTCGAGTAGGCATGGAATTGCCCTTCGATAAGGTTAGAACAGATAAATTATCACCCCGTTTTCTTGATTTCAACAAGCGAATTAGTGCCATATCTAAGCAAGGTATTTACGGCATTCCAGTCTCTTTGTCTACTGAAGTTTTAGTTTTAAATGGTCCGGTATTGCACTATATTTTGAGCAGTGCAAAGGGTAGTAGTGACAAAACGCAAGTAAGTCAAACATCATCAGGATTAAATCAACAAAAAACTCTTCAAAAACCCTTAAAGATTGATACAAGCGATTCAAGCACTTCTTCTCTTTGGACTCAAATTGAGAACGCCGCTAAAAATAACGGTAAAAAAGCAAATAATAGCAAAAGCAATAGAAGATCCACAGATCAAAGTACACAAACACATAACGATCAAGGCGATGCTTCTGAAAGTGATAAAAAGATTAAAGAATCTTGAGGCGATTATGAAGAAGTGGATGGTGGCTTAAAAGGCTTTACTTTTAAAGCTAGCATCTTTGATAATTGGCACGATTTACTCGATTTTTCTACTAGAGCGGCTAAATCTTTTAAAAAGATAAAAGATAACAATACTAAAAAAGGAACTGATATCCAAGGCATTCTTGGTGTAGATAGTTCAGCCAATTCCTTATTTACTTCTGTTTTTGCAGCTGGTAATGGTGACTATGACAACTTCTTTTACAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAGAGGTAGTTCCTTCCAAAACTTACAAAGTGTTTTTAATGATTACAAGGGTTTAATTGATCAAAACGGTCTCTTTGTTAATAAGGGCGGTTCTTACTCTTCTAACTTTCAAAAGTTTCACCAGTTAGCTTACAGTATCTCCTCTACCTCTGGTTTCTATTATTCATTTGCTGGTAATAGTGCTAAGCGCTTAAAGTTTGGTGATAATTCTTTCATTGAGTATCCACAATATACTGTTCCAATTAAAGCTCCTTCAAAGAATGGTGATGGAAATAGCACAAACAGCAATAGCGATCTTTTAGGCACTTTCACTTTAAGTAGTGGTAAAAAGTCAACAGATAAAAGTAAAAGTGATAGTCAACAAAATCAAGGCAAGAAAGTAGAAGGAACACCTAACCAGGGTAAAAAGGCAGAAGGTGCCCAGAACCAAGGCAAAAAAGAAAATAACAGTACAACTATTGAAATTTACAAGAACAAAATTCCTGATGGTAAAAATGCTGGGAAAGATGCCATCTTAATAAAAGATAACAATCTCATTAAACAGCTAGAAGATGCTGCTAAGAAAAACGGTGCAGAAAGTAATCAAAAACAAGGTGGAGAGAGTAATGGTCAGAAAGAACAAATTATTGGTTATACCACCACTGGTAACGTTCGTGAAGATGGTAATCACATTTTTAGAGTAGACAAAATTAATGACGAACAATACGATCGCAAAATTATTGTTGGCGTTACTGTGGAAACTTTAGAACAGTCTAGCACTTTGCAAAGCGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAATATAAAAGCACTGATAAAAAGAAGGTAACAATTACCCAAGGTCCGAATATTATTGGTATCCATGCTAACGAAAAAGAAAATGCAGAAACCCAAAAGTTTGTTGACTGGTTTTTAAATACAGAAGTAGATTGACCCGCTAAGGAAAATTCTTCTAATAAACAAGATCAACAAAATTCAACCAAAAAACAAACCGCTGCTGAATTCTTTGTTGAATCCGCTTCTTATATTCTGCCTTTAAAGGAAATCTTCGAAAATAAAGAGAAAAAAGAAAATACAAGCAATAGTGATAAAAATAAGAGTAGTAGTCAAAGGAAAAATACTTACGCTGAGAAAGCGCTAGAACTTTTTCAACAAATAAGTAAAGATGAAATAGTTAGTTACAGCGATCCGAGTGATTTTAGAAGTGGTAAATTCCGCGATGGGATTGGTTCTAACTTTAATGCTGCTGTAAGCTCTAAAGCAGACTTTAATAAGTTTGTTAAAGGCTTCATCGCCACTTTGGGTAGTGAAATTTAAGCGAATTTGTGAGCTAACTAATCACAATAATTTTAGGTTAAAACAAAACCTTAAAAAGGTTTAGTGAAAGAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGAAAGTAAAAAATAAAGAAATTAGTTCAAAATATCTTGCTCATATTTTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGACCTAAGTTAACACATAAAACAATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGAGAGCGCAGGAAGCAGTATGACTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAATAAGGGATATTTGTGAAATAAATCGTGGTCGAGAAATAAATGAAAAATACCTTCGAGAAAATCCAGGTGAATTTCCAGTTTATTCCTCAGCAACAACAAATGGTGGTTTAATTGGAAAAATTAATGATTACGATTTTCATGGTGAATATGTTACTTGAACAACGGGCGGCGCACATGCCGGAAATGTCTTTTACCGTAATGAAAAGTTTAGTTGTTCACAAAATTGCGGATTGTTAGAAGTAAAGAACAAAAATAAATTTAGTTCCAAATTCTTATGTTTTGCACTAAAACTACAATCTAAAAAATTTGTGAATTATGCCTCTGCAATTCCGGTTCTAACAATAAAGAGAATTGCAGAAATCGAACTCTCCTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTTTGTTTGCATTTGAAAAACTTTGCAACGATTTAACCGAGGGGATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTTCAAAATAAGAAATTAGAAAGTCTCAAATCTCTTTAATAATAAAAGTTAGTTCAGCAAACCACAAACTGATCCCACTTCTCCCCCACGCTTACGCGCTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTTCTCGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGCACAGGTATGGGAATGACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACAACCCAGGGACAACAATCGCAAAATGCATCCGGAACCGAACCCGCACCCACTTTTTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTGGGTGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGACCCACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGGCGGAATGATAAGGTTTCAAGTGGACAAAGTGACGAAAACCACACCAAGTTCACGAGCGCTACGGGCAGTGGGCAGCAGGGCAGTTCTTCAGGTACAACTAATTCCGCGGGCAATCCCGACTCGTTAAAGCAGGATAAGGTTGATAAAAGTGGTGATTCGGTAACGGTTGCAGAAGCCACTTCAGGGGACAACTTGACGAATTACACCAACCTCCCTCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTCTTCCTCCGCGCCCTGTTGGGCAGCATCCCGGTGTTGGTGAATAAGAGTGGCCAAGATGATTCCAACAAGTTCCAAGCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAGGTGTACGGTCTGAGTTCCACTCAAGATTCTAGCACCGGAGCCGGTGGCGCTGGAGGTAACACCGGAGGCGACACCAATACCCAGACTTATGCACGACCCGGAATCGGCTTTAAATTACCCTCCACGGACTCGGAATCTTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACAGCGCAAGATGTCGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGCTCGGCGGGTGGTTGGTTACCTTCACGGATTTTATCAAACCCCGGTCGGGTTACCTCGGGCTCCAGTTAACGGGCTTGGATGCAAATGACAGCGACCAAAGGGAGTTAATTTGAGCCCCCCCGGCCCTGAACCGCCTTTCGTGGCAGTTGGGTCAACCGCTTGGGCTGCGTCGAGAGTGTGTGGGATTTCAAGGGGGTGTGGCAGGATCAAGCTCGGTCCGACTCGCAAGCAGCTACAAGTACCACCACCGCAACGAAGGCTACCTTATCGGAGCACACCAATGCTTTGGCCTTTCAGGTGAGTTATACCGACCAGGATTCGTACAAGGCTTCCACTCAAAGCTCCGGCCAAAACCAAAACACCTCCCCCTACCTGCACTTGGTGCAGGGAAAAAAGTAGGTTCCTCTGATAAGCTTTCAGACGATCTTAAAAAACTGTTGGACCCCAACGAGGTTCGCGCCAAGCTGCGCCAAAGCTTTGGTACAGAAAATTCTACCCAACCCCAACCTAAAGCCCTCAAAACAACCACACCGGTATTTGGGACGAATAGTGGTAACCTCGGCAGTGTGCTTGGTGGTGGGGGTACCACGCAGGGCTCAGGCTCAGGTCAATCTGGTGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAGGAACTTTTACTTTCAAAAAATTTTTGAAGTTATTTTGGCACTTTCAAACTTTAAAGACCATTTAAAAGGCATAAAAGGATAGTTATGTTTTTAAGAAATATTTTGCGATTCAGTTCGCTTTTGGTTTTGTTTGGTTTTGAGTCTTTTCTTTTGAATGTTCAAAAATTCTTTGAATTAATTTTTTGGCAACTTTTCCAATTTATGCAAATTTACATGATGTAAAACACTCACGATGATAGTTTTTAAAAAATATTTATTTTATTTAGATAGCCAAATAATCCAGTGATCTGTTGCTGCCCACCTCTTTAAACACTTTTACGTTTTTCCAATTAATAATAAAGGAGGTCTTCACCATTAAACGCACAATTTCACTTACTTTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTATGACATGGGATTTAGTGACAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAATAATCAAAGGCTGATAAAGATTGAAACTACTTTAGCTACTCAAGGTGAACAATTAAATCAATTAATTAAAGTTGTTATTCTTCAGGGCGAACAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAAGGAGAACAAATAAAAGCTAAAGGAGAACAAATAAAAGCTCAAAGCGAGCAAATAAAAACTCAAGGAGAAACGCTTAAATTGATCCTACAAGCACTTGGAGGAATAAATAAGCGCTTAGACAAAGTTGATCCGCCTAAATAGCTTTAGGCTAAAAAATAAAAGTGCCAGCAATTAATCTATAAAGGTTAGTTGCTGGTATTTTTATTCATTTGAAATAAATAAAAAAGCCATCTGCTCAAGGCGAAAAGCAGACAACTCAAAAATTTTTTAATGATTTTGAAAACTAACGAAAAGTGTTAGTTTGAGTTGTAAGACAGATATGAATGTTTATTAATTCAAAAAAATGTTCGTTGCTTAATGAAAGATGTGCTGAAAACCGTAACGGTAAGATAGATTTGTAAAAAGAGTAAAGAACAAAACCATGTACGCAATAACTAAATTGATGGGAACAGAAAATCAGGCTTTCAACACAGAATTTTCGTTTTCCTGCTCCCAATAAATAAATTGATTTAGTTTTTTAAATGTGACGTCAAATTTCAATAAAAAATCAAAATTTCAAACCAAAAACTATAACACACCAAGCTCATTGATTTTAATCTCCAGTTTTTTCTCATTTAAAAAGGATCAAAATTTGTTTTTAATAGTTTCGATTTTATGTCTTCTTTAATCACTGAATTTTTAGGAATTAAACTTGTTTAATCTTTATTAAGATTTCTCCAAATTTTGGGTTTTAGTGCATGAAATTTAAGTATTGTGCCATCTTTTTCAGTGGCTTTCTAGGTCTTTCTGCCATCTTAGCTGCTTGTGGAGCGCGGGGCAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTTCTCTTTAACTTCAAAAAGTGCATCAAACGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCATATTGAAATTACCCAAATCGCTGGTGGTTATGATGGTGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAGGACACCACTAACTTTTACAACCTTATCTTGAATTATCCTGACTTAGTTTCTACTTTAGGTCGTGTTGGGATGGAACTACTGTTTGATAAAGTTAATACAGATAAATTATCCGACCGTTTCTTAGATTTCAATAAACGAATTAGTGCAATTTCTAAACCAGGGATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAAAAGTGCAAAAGGTGATAGTGACAATGTTAAAGTAAGTCAAAGGACTGGAGAAACTACTCAAAAAAGTAAAGTAACTAATCCTTTGAAAATTAATACTCAAAACGATCCTGCTACAAAAGATCTTTGAGAAAAAATAGAAGCTTCTGCAAAAGCTAATGGTAAAAGCAATAAGGATGGACAAACTAAGGGTAAAAAGAAAGTAGAAAAAAGTTCATCTTCTTCGTTAGTTAATTTAAAGCAGTCTACGGACCAAACAACGACAGATGACGGTTCTCAAAAAAGTGATAACAAAATCAAAGAATCTTGAGGCGAATACCAAGAAGTAGATGGTGGTCTTAAAAATTTTGAATTTAAAGCGAGCATCTTCGAAAATTGACATGACTTACTTGATTTTTCTACTAGAGTGGCTAAGTCGTTTACAAATGTAAAGGGAAAAGATATCAAAAAGGGAACTGATATCCAAGGTGTACTTGGTGTTGACAGTACGCCCAACTCATTATTCACCTCTGTTTTTGCAGCTGGTGGCGGTGATTACAATAATTTCTTCTACAAAATTGAAAACGGCCGTGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCCTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTTCAAAAGTTCCATCAATTAGCTTACAGTATTTCCTCTACTTCCGGTTTTTTCTATTCGTTTGCCGGTAACAGCGCTAAACGCTTAAAGTTTGGTGATAACTTTATTGAATTTCCTCGTTTTACTCAGGAAATTAAAGCACCTTCTACTGAAAATGGCGGCCAAAGCAATCTTTTAGGAACCTTTGAAGTTAAAGATAGCAGTAAATCTAAGGAAGTGAAAAAAAACTAATAGGAAAGAAGATGGTGCTCAAAATCAAGGTAAAAAAGAAAGCGATAAGAAAACCATTTACCTTTACAAAAGTCAAATTCCATCAGACAAAACAGAAGGCGAAAATGCCATCTTAATCAAAGAACAAAACGTTATTAATCAGCTAGAACAAGCAGCTAAAAAAGATGAAAAAGGAGAGACTGTTACAAACAAAGTTGCATCATTAGAAACCAAAGCTGCTAATGCCAAAAAGGATAGTAGTAAAACCATTATTGGTTACACCACAACTGATAATGTTCATGAAGACGGTAAAAACATTTTCAAAATTAACAAACTCAAAACTGAAGACTATGATCGTAAGATTATTGTCGGTGCCACTGAAGAAGTGTTAGAGCAATCTAGCACATTACAAAGTGATGAAGCAATTGTGTTACCAGCTCCTGGTAAGTATCAAAGTGGTGACGCTAAGAAAGTAACCATCACCCAAGGTCCTAATATCATTGGCATTCACGCTAACGAAAAGGAAAACGCCGAAACGCAAAAGTTTGTTAACTGGTTTTTAAACAATACAGAAAGTTGAGAAGTTAAAAATGGAAAAGATTCCTCCACTAAACAACAAACTGCTGCCGAATTCTTTGCTGAGTCAGCTTCTTATATCTTGCCTTTAAAAGAAATCTTTGACAAAGATAACAAAAAAGCTACTGAAAATAAGGACAATAATACTAGCAATAAAAAACAAGCTAATACCTATGCTGAAAAGGCGTTAGATTTGTTCCAACAAATTAGTAAAGGTGATATAGTTAGTTACAGCGACCCTAGCGATTTTAGAAGTGGTAAATTCCGCGATGGGATTGGCTCTAATTTTAACGCTGCAGTAAGTTCCAAAGCTGATTTTGACAGGTTTGTTAAAGGCTTCATCGCCACTTTGGGTAGTGAAATTTAAACGAATTTGTGAGCTAACTAATCACAATAATTTCAGGTTAAAACAAAACCTTAAAAATGTTTAATGAAAGAAATTAAATTGTTACTGTTGTGCAAATCAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAACAATCCAGGCGAATTCCCTGTTTATTCATCGGCTACAGTTAATAATGGTGAAATTGGCAGAATAAAAGATTGTGATCTTAAGGGTGAGTATGTGACTTGAACAACTGATGGCGCTCAAGCTGGTAGTGTTTTTTATCGTAACGGTCAATTTAACGCAACCAATGTTTGTGGAATTTTAAAGGTAAATAATGATGAAATTTACCCTAAATTTTTAGCCTATGCGCTTAGATTGAAAGCACCTAAATTTGTAAATTATGCTTGCCCTATTCCTAAATTAATGCAAGGAACTTTAGCAGAAATTGAGCTAGATTTCCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGTCTTCTATAGCGATTATTTACTTAACCCAAAAAATTGAAAATAGGAAAATAAATATTACAAGCTGGGAGAAATAGCTCAAAAAGTTTTAGTAGGTGGTGAAAAACCTGCTGATTTTAGTAAGAAAAAGAACGAAGTTTATAAATATCCAATTCTTTCTAACAGCAGTAAAGCTGAGGAATTTTTGGGTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGTCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTCCCTAAAGAAGAATTTGATATAAGGTTCTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAATATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCACCGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTCTTCCTGCTGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTTTTATTTAACTGAATTCAAAAACAAAAAGAACTAGTTGAACAAGCATCCACTAATTAACGGGATCTTTAAGGTAGAGTAGTTCTAAATTTTGGGGATTTTCCACTAGTTCAAACCGTTCGATGTTGTTTAGGAATAACTCCTCAATACTGTCGATGTTTTCAAAGTTTTCATATAACGGTAACTCGGTATTTGCTTTGCACAGCTTGACAAAATCTGGTTTAGCTAAGAGTGCTATTTCACTCGTAGTTTCGGTAAACAAACCACAGTAGTTCTTTTCATTGCCAGCACTAATTTTGCTAATGCCACAGCCATAAGGAATTTGAAGGCGGAGTGAGTTTAAAGCAAAGAGTTGACAGTTAGGGTTTACTGTGCACCAAGTTTTAGCAATAATGGCACCAACGCGTTGACCAGTAAAGCTACCCGGTCCAGTGGTTACGTAAAACTGTCTAACAGTGTTATTGCGTACTTTGTTTTTCTTTAACAATTTTTCCAAGTGATACACAGCCAACTCCGTGAGGTTTTGTTGTACTGGTATTGTTAGTTGATCCACCACAGTATTCGTTTTAAAATCCAACAGTACGATATTGAGGTGTTTGTAAGCACAGTCCAAAAAGAGCTTGTACTTATTGAAAAAGCGCATAAAGGGAATTAGCAATTTTCAAGTTTAAATTATCTAACTTATCGCTAAAAGTTTTATCTAACTGTGCTTCAAAACCCATTTCCTTATTCAAAGTGGGGTGGATAAAACTAATTTGTTGCGCGTGTAAATACTGGCCATATTCAGTAGCTTGCTCACCCTTCAAACCGTATAAGGGATCGTTGTAAACGGGGTGTTTAATAAACTGCAAGTGTACCCTAATTTGGTGAGTTCGTCCGGTTAACAGTTCACACTTAATTAAAGCAGCCTTTTCGTTTTGGTTTAACACCGTAAACTTAGTAAAAGCATTCTTGGCCTTGTTAGAACTGTTCCCCACCTTTCACATGACCTTATTGTTACCCACTCGTTCTAACGGGGCGCTAATGGTACCAGACAAACTGTCTAGGGGAAAATGGACTAAAGCTAAGTAATAACGCTTCATAACCCGTTGCTGGAGCTGTTTTTGCAAGTGCAGCAAGCTACTTTGGTTTTTCGCTAGCATAATCACACCACTCGTATCACGGTCTAGGCGGTGGACTAAAAACACCGGTTGGTGGTTGCAGTGAAACAAAGCGGCACCCAGTAAACTTTCACTTTCATTAAAACTAGTCGGGTGGCTAATTAAACCATTGGGTTTATTGACCACCATAATTTGCTCATCTTCATATAAGATGTCCAAGGGAAAGTCATAGGGCTGGACTTCAACTGGCACTTTTTCAAACAGTTCCTCACTCCAATTAACGTGTACCCGGTCTTCTGGTTGCACTAATCAACCATTTTTAGTAATTTTTTTACCGTTAACACTCACCAAACCATCGACAATTAACTTGGCTACTTTAACCCGGGAAAGGTTGAGTAGTGTGGCCAAAAAGGTATCTAAGCGTTGGGCTGTAGTAACACTAAAAGTTTGTTCCATTAAGATTTAGTTTGCTTCATTTCTTTGAAGAACTGAATTAAAAAGGAGAGGAACAGACCAATAAAACTAAAGGTAATGCAACAGTCCGCTAAGTTAAACACGGAATTACCTCCACTAAAGACAAAGTAATCTAGTACTTCACCACTACCCGAGGTTAACCGATCAAAGAAATTACCAAGTGAACCAAACGCTAAGGTAGTAATTCAAAAGATGTAGTTGTAACTAGTAGAGAAAACCAAAAAGAACAGTGCTATGAAAGATAGAAAACCCTGGAGAAAGTAAACAAGTCCTGGTTGGTCTGCCAATAAGCTAAAACCAACCCCCTTGTTACCGATAACATAGATACTAATAAAGGAACTGCGAGCTACATCTGTACCATTAGCACCATTAATACCCTTCTCACCATTTAAGGCAGCACGCAGCATAAATACCTGAAACAAAATGACAAAACCGACAAACAGGATTAAGGCAAGCTTATAGTACAAAAAGGGTTTGCGGTTAGCAAAAAGGATTTGCTTTAGCAATTTTGAAAAAAAAGTTGGGGCTTTTGCCATCTTGATGAGCTTAATTTTAAAAATGTAAAATAACGGCCATGGATTTGCTTACAACCGCAAACAAAACGCCGCGAATCGCCAAATCACGTCCTGTTCGAATTGCCATCATTATAACGCAGAAGACTAATGATCTGCACGCTACTGTACCCTGCTTTTTATGGCGCAAAGCAAGGTATGCTGTTGACCTGATTTCCGCTGAAGCCAAAGCTAGCATTATGTTAGAGATGGGGATTCATGTGCGCTGTGACAACACTTTGTCCAAAACTAACTTTAACCAGTATACCGCTGCTTTTATTCCCCATGGCAACACTACTAGGTTGGTGGAAATAGACAAACTAAGAAAGGACTTGGAGAAGTTTGTCTATAAACCCAAGGGACCAATGCGCTGGTTGTTTAGCAGTGGTAATGGTGCTTGTGTTTTGAAGGAGTTTGACTTGATTGCCCCTGACCAATTAGTCACCGTTCAAAACGAAAAGGAAATGGTTAAACTTTTGGGAAAAAATTTCATCAAGCAACCAGTACATGTGGACAAAAACATTATTAGTTGTGCCAATTCTTGTGGCTTGACTAAATTCTCCTTTAAGGTAATTGAAGAGCTTTCAGGAATAGAACTAGCGCGCAAAACGGCGAATCTTGTTGACCATATCTACAAAGGATAAAACCAACAATTTAAAATTACCTAACGCGCTTGCGCTTAAAAAATCGCTTTGAACAACACGAAAAACAAGTCAGATTGACAGCTCTTTTTGGAGGACTACCGTTTTTATCACGAAAAGGAATTTGATTGAATTACTTACCTCAATCACTGCCTCAACAGCTATCCTGACTTTGACATTTTAAAGTTCATTCGCAAATACGGTCCGGAGTGTGAAAAGAGCTTTTTATCACTCCAAAGTAAAACTAAGGCCGATGTTTATGGCGTGTTCACTAAGCAAATTAAAGCTGGCAGTGTTAACGAAGTGTTAGCGCAAAAATTAGTCCAACTCGATGCGCTGCGCACTAACTATCTCATTGGCGCTTTGTACTCCACCAATAAAACACAAAAGAAGCTGTTTAAGCAATCGTGAAAGAATGCTAAAAAGCAGGGTTACACAAAGCAAGAGTGGCTAATGACCTTAGTCGGTTTGCCGTTTGAAAAAGGGGAATATCATAAGCAACTGTATGCGCATTCCCGTCAAGAGATTTTGGATCTAGTGGAAGCGGTCAAAAAGCTGTATCTCAGACCAGAAAAGGATGACAAACTGGAGTTTGCTGATTCTTCCAAGGTAAGTGAGTCTAAGAGTATTAAGGTAACCAATGCAGTAACCTTACCAAGTGATGATTTAGATAAAGAGCTGTTTGAATTTAGCGGGGAAGGGGGTGATGAATAATGCCAAAGATTGAAGTTAAAAACGATGATTTAGAGTTAGCTTTAAAAAAGTTTAAACGGGTTTCGCTAGAAATTCGCCGTTTAGCACAGCGTCACGAATACCACCTTCGAAAAGGAATGCGCTTGCGTGAAAAGCGAAAGATTGCGCAGAAGAAACGCCGTAAGTTTCGTAATATGGTCTAGTTACTAGCACCTTACCAGTTTTATATGGCTAACGATAAAAAGGATAAGATTATTTTGAGTGGTGAGTTAACTAACCATCGCTTTAATTTTACCAAGGATGGGGAAAACGGCTATAGTGCTTATGAAGTAGACCGTTTTCTAGATCAGCTAGTTCACACTTTAACCCATTACGAAGCGCAGCGTAACCGTGAGGAGGAGATGAAAACGGCTTACGAAAAGCTCTTTCAAGACCGCGACGAAATTTTAAAGCGTTGCTCCAAACTAGAAGCAGAGCTCAATAACTTTTATGAAAACGGTTATTCCAACCGTGTGTTAATTAGTCGGGTTCAAGCCTTGGAAAACAAGATCGAAAGCCTCCCTAGTGGTCAAAATGACCGTCTAGAGCGCATCGAAAAGTTGCTAAAAAGGGTGATTAAGCACTGGACTGACGGGGAGGATTTATCCTATGGTGACTTCGATGATGATTTCTTTTAAAAATGATTCTAGGCATAGGGATTGATTTAGTCGAAATCAAACGCTTTGAACAATTAGCTAGACAAACTGATAACTGTTTTGCGAAACGCTTGTTGACTTCAACGGAGTACGCGCACTATGCGAAGCTGCGTAAAGATAGTGAAAAGTCGAGTTTTTTAGCAGTCCACTGGTCATTAAAAGAAGCGATTTATAAGGCTGTTAACCACATCAAGCCATTGTTTAGCCAACTGGAAATTACAAAGAAAAACCAACGTTATAACTGCCAGATTGACCCTAAGATTGAACTTTTGCTCTCGGTTAGTTACAGCAGTAATAACATTACTGCCATCTGTTTGGCACAACAAACACCATGAAAAAATTAACACAAGCTTTTTTGCGTTTTTGTCTGAGATTCTTACAACTGCTCAGTTTAGTTTTGGTTTTACCCGTTTTTGTGTTAATGCTCATCTCTAGCCTAATTAGTGCTAAAAATTATGAAAGCATCCCGGAAAACTATCCCCCAGAAATCCGCTTTAAGAAGGTGTACCGCTTGGTATCACTTTTCCTCTACATTAAAGGAGTTAAAGTGGTTATTGTTAACCCTGAAAATGTGCCTAAAAAGGCTGTTTTAGTGGTAGCTAATCATAAGTCCAATTTAGATCCCCTCATCCTCATTAAGGCCTTTGGTAAAACTGAAGGGGTACCACCGTTAACCTTTATTGCCAAGATTGAACTGCAAGATACCTGACTGTTCAAGATTATGAAGCTGATTGACTGTGTCTTTATTGATCGAAAAAACCTGCGTCAAATGGCAGCATCCTTGGAACAACAGCAGCAAATTATCCGCCAGGGCACTGCTCTGTGTGTTTTTCCTGAGGGTACACGGGTACTCTCTAGGCAAATTGGTGAGTTTAAATCGGGCGCTTTAAAGGTCGCTTACAACGCCTTTGTGCCGATTGTGCCACTAACGATTGTGGGTAGCATGGGACACATGGAGTCCAAAAAGCGTTTACAAAAAGCCCAGGTAGAGCGCGATCGTGGTTATAAAATCCAGGTCATCTTTAACACACCTATTAACCCAATTAACTTTAACCAGATTGATTCGCAAAATGTGGCTAATAATGTCTGGCGTGAAATTAGTCAAACTTATGCGCAATATTGCCAAGACTAGATGATCACGTTAATCTGGTGCCAAGATAAACACTTCGGGATTGGGAGAGACAACACCATTCCCTGAAAGTTAACGGAAGCCAACCAACACTTCTATAACACTACTAAGAACCAAACGGTTGTAATGGGTTACAGCACCTTTCAGGAGTTAGGTGACAAGCTAACTGATCACAACGTGGTGGTGTTGAGTAAAAAACACTTTGAAGAATTGCAAAACAACACCAACATTAAGGTGTTCAATTCAATTGAAAAGCTCTTGCAACACCACTTTAACCGTGATTTGTATGTGATTGGGGGCAAGCAAATCTTCCACCACTTTATTGAACTGGCTGATCGTTTAATTATTTCGGTTTTACCAGTGGACTTTAAGTGTAACCTTCGCTTAAAGTTGGGCTTGGACAGCTTTGAATTAATGCAAGAACAACAACACAGCCAATTTAAGGTGCAGTACTGACACAAAAAGCACCCCGAACGTTTATCGTTTAATGTTTTTTTGGAAGATTACAATGGGACTTTACCAAACTTGCTGGAACTTTTAATTGATAAGAAGTTCAACTTGCACCAGGTCGATATTGCCAAAATTACGACACAATACCTCCACTTAATTAACACTAACTTAAATAAACAAGCAATTGAACCAATTACCGACTACCTTGTCATTACTAGTCGAATTGTGGAGCAGAAGGCCAATAACTTACTGCAAATTAATGATATTGCCCTAGACTCTGACTTTTTAGACAACAAGCTGCGTGATAAACTAGTAGCACAGTTAGTGGAGTACAAACGTTACCGTGAATCACTCGATGACTTTGAGAAGCTAAGGATCAATCGCTTAGCTTACTTTTCCAAAGATAATGACTTTAACCGCTTTATCCAAACAGTTGACAAGAGTAACACAGAACCCGTGAAAATTGAGGACGAGCTACCCAATTATGTAAGTGTCTTAAAGTTGCATCACGCCATGAATAAGCTAATGCAGCGCTGAAGAGCCCAGTTTTTAGCGAACAAAAACATTTCAATTCAGGAGCTTTCAATTGAACAAGTCCAAGCGGAAATTTTGGCTACAATTAAGCAATTTGGCTATCATAGTGTTTCGTTAAAACGGGTACTGTTAAAGGTCAACCACCACATTTCCTTAATGTATTTCATTACCGCTTTTGTCGCTTTATTGGTGTTAATTAACAACCAGATTATCGACATTGAGCAGACTAGCTTTGATGATGAGCTTTACATCTGCTTGTTAGACAGTTCGCGGATTAAACAGCTCCAAGAAACGCCAGAAGCCATGGTGGAACGTGCTGTGAAACAACGCCAGGAAGCACAGGAATTAGCCCGTCAAGTAGCTAGAGAAAAAGCAATTGCCAACGCGCAGAAACGCGAAGCTTACCTAAAGGCAAAATATGGCAAGGATTACTTAACACGTGAACAGTTTTTAAAACTGTCTCCCGAAGAACGTGCTGCCCATGTGGCTAAAATGAAACAATTAAAACTAGTAAAGAACGACAATGGACGCGACAATTAAGGTAACTAAACCCGTTTTAAAACAAAAGGATAGCAGCGCTGCTAATTTAGTTGCTGCCATTTATGGGTTGTTGTTTGTAAGTGGCGAAAAGGGCTTAACGTTAGCGGAATTAAACCGCGTATTGCGCAAGGTGGGTTTGGAAAAGATTAAGGCCGCTTTAGTGCAGTTAGAGCGCAAGTTAAGTTTAGATGATGAAAGCGGGATTGAAATTAAAAAGTTTGGTCACAGCTTTCGCTTGGTCACCAAAATGGAGATCAAGGACTTTATCCACCGTTACCTACCCAACAAGATTAAAAATCCGCTCAATTCCAAAACAATGGAAGTGTTAGCAATTATTGCTTACAACCAACCCTGCACCCGTCCGCGCATTAATGAAATTAGGGGTGCAGATTCCTTTCAGATTGTCGATGACTTACTAGAAAAGGAGTTAATTGTTGAATTGGGCCGCAAAGACACACCTGGTCGTCCCTTTATTTATGAAGTGTCGCCTAAGTTCTATGATCTGTTTGGGATTAACAGCTTGGATGAACTGCCTAAAGTCGAGAATTTTGATCTCGACAAATTCCGCCAAGGTAGCTTTTTTGACTCTAACCGTTACGGGGATGATTAAGGTTATATAATTAGCGCAAATGAGTCCAAAAACAACCAAAAAAATTGCCATTTTAACCTCTGGTGGTGATGCTCCAGGGATGAATGCCACACTAGTTTACCTAACGCGTTATGCCACTAGTTCTGAAATTGAAGTTTTCTTTGTGAAAAACGGTTATTATGGGCTGTACCATGATGAGTTAGTACCCGCTCATCAATTAGATTTGTCAAACAGCTTGTTTAGTGCTGGCACCGTGATTGGTAGTAAGCGCTTTGTCGAATTTAAGGAGTTAAAGGTCCGCGAGCAAGCAGCACAGAATCTTAAAAAGCGCCAAATCGATTACCTAGTGGTTATTGGTGGTGATGGTAGTTACATGGGCGCTAAATTGCTAAGCGAGTTGGGCGTTAACTGTTACTGTTTGCCGGGTACGATTGACAACGACATAAATTCGAGTGAGTTTACAATTGGTTTTCTCACGGCTTTGGAGTCGATTAAGGTCAATGTCCAGGCCGTGTACCACACTACGAAATCACATGAACGGGTCGCCATTGTGGAGGTGATGGGTCGCCATTGTGGTGATTTAGCCATTTTTGGTGCCTTAGCCACCAATGCTGACTTTGTGGTGACTCCTAGCAATAAAATGGATTTAAAGCAGCTAGAAAGTGCTGTCAAAAAGATCTTGCAACACCAAAACCACTGTGTGGTGATTGTTAGTGAAAACATCTATGGCTTTGATGGTTATCCCTCACTTACAGCGATCAAACAGCACTTTGATGCTAACAACATGAAGTGTAACCTGGTGTCCTTGGGTCACACGCAACGCGGATTTGCCCCTACCAGCCTCGAGTTAGTCCAAATTAGTTTAATGGCGCAACACACGATTAACTTAATTGGACAAAACAAGGTTAACCAGGTCATTGGTAATAAGGCCAATGTGCCCGTTAACTATGACTTTGATCAAGCCTTTAACATGCCACCGGTTGATCGCAGCGCTTTAATTGCAGTCATTAATAAAAATATTATTTAACTGATATGATTCACCACCTAAAACGCACTAAGATTATTGCCACCTGTGGTCCGGCTTTAACTAAAAAGCTATGAACGCTAGCAATGTTAGATGACCCAGCTTACGCTGCGATGAAAGCAGAAGCTTACGCCAACATTGAAAATATTATTAAAAACGGCGTAACGGTAATCCGCTTAAACTTCTCCCATGGTAACCATGAAGAGCAGGCTGTAAGGATTAAGATTGTTCGTGATGTGGCTAAAAAGCTCAACCTCCCGGTGTCAATTATGTTAGACACCAACGGTCCGGAAATCCGCGTCTTTGAAACGGCCCCTGAAGGGTTAAAGATCCTCAAGGACAGTGAGGTGGTTATTAACACCACCACTAAGGAAGTGGCTAAAAATAACCAGTTTAGTGTGAGTGATGCTAGTGGCACTTACAACATGGTCAACGATGTTAAGGTGGGACAAAAAATCCTCGTTGATGATGGTAAGTTAAGCTTAGTCGTAAAGCGGATTGATACGAAGAACAACCAGGTTATTTGTGTTGCCCAAAATGACCACACTATCTTTACCAAAAAGCGCTTAAACCTCCCCAATGCCGACTACTCAATTCCCTTTTTAAGTGCTAAAGACCTACGCGACATTGACTTTGGTTTAACCCACCAAATTGACTACATTGCCGCTTCCTTTGTCAATACAACGGAAAACATCAAGCAACTGCGTGACTACCTCGCTTCTAAGAATGCCAAGCATGTCAAGCTTATTGCCAAAATTGAGTCCAACCACGCTTTAAACAACATTGATGGGATTATTAAAGCGAGTGATGGCATTATGGTAGCACGGGGTGATTTGGGCTTGGAGATCCCATATTACAAGGTGCCTTACTGACAACGGTACATGATAAAAGCGTGTCGTTTCTTTAACAAGCGTGTTATTACCGCAACTCAAATGTTGGACTCTTTGGAAAAAAACATCCAACCAACTCGCGCTGAAGTTACTGATGTGTACTTTGCCGTGGACCGCGGCAATGATGCCACCATGTTAAGTGGAGAAACCGCAAATGGGGCGTTTCCCCTTAATGCTGTGTATGTAATGAAGATGATTGATAAACAGTCCGAAACGTTCTTTGATTACCAGTACAACTTGAACTATTACATGGCCAACTCCAAGGCCCGTCACAGTGAGTTTTGAAAGCAGGTGGTGTTACCGTTAGCACAAAAAACAGCACCAAAACGCAAACTGATTAACAGTGACTTTAAATACGACTTTGTGGTGCACGCCACCAACAACCTTAATGAAATCTATGCCTTGTCCAACGCTCGCTTAGCGGCTGCGGTAATTATCTTGACCAATGACCCCCAAGTATACACCGGGCACGGCGTTGATTATGGCATCTTCCCTTACTTAATTGACCAAAAGCCGCAAAGCTTAAGTAAAGCTGAGTTTAAGAGCTTAGCTAATGTTGCCATTAAACACTACCAGCAGCATGGGGAAATTAGTCAATTAAAGCAGTGTTTGGGAGTATTTCACAATAAGATCATTAGCTTATAAGCGCTAAAAATTAATTAGAGTTTGTAAAATATTCTACACGGTGCCTTAGCCAAGTGGATTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCATGCACATGAACAATAAATCCACTACAAGGTGAGAACCCTTGCATGTGCAAACGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCATAATGGCTGGATACCTCAGTTGGTTAGAGGACCCGGTTCATACCCGGGTTGTCGTGAGTTCGAATCTCACTCCAGCCACCAAAATCATTAAGGATCTATAGCTCAATGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCGAGTCCTGTTAGATCCACCATCTGGTTGCTGTGTTAGAATAGTACTTGCCGCATTCACCTGGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTGAAAAGCACGCGAGGGTTCGAATCCCTCAGTCTCCGCCAAACCACATTGATCGCGGGATAGAGCAGTCGGTTAGCTCGTCAGGCTCATAATCTGAAGGTCGGGGGTTCGAATCCCTCTCCCGCAACCACGGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCACGGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCATAAAACTTTCTTACCTTACCTTTAAAACTAAGGGTAAGGTTTTTATTGCTTACCACCTTTATTTAAGGCAGTACAACAGCTGTAAATTAGTTGCCCAATTCTTTTTGCAATCAAGTTCACTTTGCCTTTTTTTCTTGGGCTTTTGTGTGGACGTGTAACAATTAAAGCAGTAAGCTTAGCCTTATTACATGTAATATGAAGTACAACATCAACGTTCATTCCGAAATTGGTCAACTGCAAACCGTTTTAGTCCACACCCCTGGTAATGAAATTAGACGGATTTCACCAAGACGGTTGGATGATTTACTGTTTTCAGCAGTAATAGAACCAGATACTGCCATCCAGGAACACCAAACCTTTTGTCAGTTACTGCAAGAACAAAACATTGAAGTGGTGCAACTGACTGATTTAACAGCCACCACCTTTGACAAGGCAAATGCTACAGCGCAAAACCAGTTTATTGAAACTTGACTAGATCAAGCAGAGCCCAAGTTAACCCCAGAACACCGTAAAGTAGCGAAGCAGTACTTACTGGAACAAAAAGCAAAATCGACCCTCAGCATGGTACGCTCGATGATGGGTGGGATTGATAAGCGTAAGGTAGCTGCTGCTAACACCATTAACGGTGATTTTTTAGTAGATCCGATGCCTAACCTTTACTTTACCCGCGATCCGTTTGCCTCGATTGGCCATGGTATTAGTATTAACCGGATGAAGTATTTAACGCGGCGGCGTGAAACCTTATTTGCTAGTTTTATTTTGCCAACCACCCCGATTATTGCCGCACGCAAATTTACTTTAAACCAATTGACATGGGCACAATTGAGGGTGGTGATATTTTTGTATATGACCAACAAACAGTGGTGGTGGGGTTGAGTGAACGGACAACCGAAGCTGCCATTAATGTTTTAGCGAAAAAGATTCAACAAGACAGTAGCACTTCCTTTAAGCGGATCTTTGTCATTAACGTTCCACAACTACCGAACTTAATGCATTTAGACACGTGGCTGACAATGTTAGACCGCAACAAGTTCTTGTACTCCCCGAACATGTTGGCGGTTTTAAAGGCGTGGCGGATTGACTTGACTGATCCGGCTTTAAAGTGAAATGAAATAGCTGGTGATCTTTCAACCATCTTACACACCATTATTGGGCAAAAACCTATGCTCATTCCCATTGCCGGAGCGGATGCTAACCAAACCGAAATTGACATTGAAACCCACTTTGATGGCACCAACTACCTCACCATCGCTCCATCGGTAGTAGTGGGGTATGCGCGCAATAAATTAACACACCAGGCCTTGGAAGCGGCTGGGGTTAAAGTAATTGCCTTTAAGGGGAACCAGCTGTCTTTGGGAATGGGTTCTGCACGTTGTATGTCGATGCCATTAGTACGCAAACCACTTTAATTTTTCTTTATTTACTTATACAAATTTATGCCAATTAACTTAAAAGGTCGTAGCCTTGACTCTGCTTTAAACTTTACTACTGCTCAAATTAACTATTTAATTGACCTCGCCATTGATTTAAAACGCAGTAAATACCAAGTTGCACATCCAGAACCGTCCCTTAGTGGGGAAGAACATTGTTTTACTGTTCCAAAAAGATTCTACCCGTACCCGTTGTGCCTTTGAAGTGGCTGCCTTTGACTTGGGTATGGGTTGCACTTACATCGGCCCTTCTGGTTCCAATTTTGGTAAGAAGGAATCGATTGAAGACACTGCTAAAGTGTTGGGTTCAATGTATGACGGAATTGAATTCCGTGGCTTTAAGCAAAGCGATGTTGATGCTTTGGTAAAGTACTCTGGTGTTCCTGTTTGAAACGGTTTGACTGATGCGGAACACCCTACCCAAATGCTAGCGGATTACATGACGATTAAGGAGTTGAAGGGTGATTTGAAGGGGCGCAAAATTGTCTTTGCTGGTGATATTAAAAATAATGTGGCACGCTCGTTGATGATTGGGGCAGCTTTTGTTGGTATGGACATAGTGCTAGTAGGTCCTAAAGCACAGCATGAATTAGTTCAAAATGGTGCTGGGTATAAAGAGGTCTTTGAGCAGTGTCAAGCATTGTTTCAACTCAATGGTGGTAGTGTTAGCTTTAGCACTGATAAGCTGCAAGCGGCTAAGAATGCTGATGTAATTTACACCGATGTGTGACTGTCGTTGGGTGAAGACTTTAGCTTATTTGAAGAACGGATTCAGGAGCTGGGTCAATTTCAAGTTGACATGGCTATGATCAAAGCAGCTAAGAGTGATGTGATCTTCTTGCACTGTCTCCCCGCTTTCCATGATGATCACACTAGCTTTTCACTGGAAATTAAACAAAAGTTAGGTAAAAAATATCCGGTGGTGAAAACGGGTGCGATGGAAGTGACTGATACGGTGTTTCAGTCCAAGCATAACATGGCCTTTATCCAAGCAGAAAACCGCTTGCACACGATTAAGGCCGTTATTTTAGCTACTTTAGGGTATTAAAGATGCAAAAAATTGTCATAGCATTGGGTGGTAATGCATTAGGTAACAATCCCACCGAACAAAAGGACTTGGTACAACTACCAGCACGACAGGCGGTTGCTTTGTTAAAGCAGGGTTATCAGATATTATTGGGTCATGGTAACGGTCCACAGGTTGGCATGATCTACAACGCCTTTAGTGCGGCCAAACAAGTCAACCCTAATACACCCACTGTACCATTTGCTGAAAGTGGGGCAATGTCTCAGGGTTACATTGGACTGCATTTGTTAACAGCATTGTACAATGAGCTGCTACACCAAAAACTACCCCATCAAGCCGTGTACTTTTTAACACAAACCTTAGTAGAAGCGAGCGATCCTGCTTTTCAAAACCCAAATAAACCAGTTGGTCCGTTTTACAACACAGAGGAAACAGCACGCTCAGCTAATCCAAACAGCACCGTGGTGGAAGATGCAGGGCGGGGTTGGCGTAAAGTAGTAGCGTCCCCCAAACCAGTTGATGTTTTGGGAATTGATGCCATTAAAAGTAGCTTTAACCAAGGCAACTTGGTGATTGTTGGTGGCGGTGGCGGTGTACCCACAATTAAGACCAAAAGTGGTTATGCAACGGTTGATGGGGTGATTGATAAGGATCTAGCATTGAGTGAAATTGCCATTAAGGTAGAGGCTGACCTGTTTGTTATTCTCACTGCCGTGGACTTTGTCTACATTAACTATGGTCAACCAAACGAGCAAAAGCTAACCTGCATTAACACCAAGGAAGCTAAGACCTTAATGGCCGCAAACCAGTTTGCTAAAGGTAGTATGCTCCCCAAGGTAGAAGCGTGTTTAAACTTTGTCCAGTCGGGTACCAATAAGACGGCCATTATTGCCCAATTAGACCAATTAGCTGCGGCCATTGCGGGCAAGATTGGTACTAAAATTGTCAAGTAGTTATGAAACAACAAAAACCCAAAATTAGCTTTATAGCAGCGATGCTAATTGTTATCGGCTCGTCCATAGGTGCTGGTATCTTTTTTAAATCCAGCACTGTTTTGGAAAATTCCCAGGCCTCTTTAGTGCTAGCGATTTTTAACTGGTTGGTAGCTTCAGTGGCAGTGATTGCTATGGCCTTGGCATTAATTGAAATTGCCAGTGTTCGCAACGATAATCTCTCGATTATTAGCTGGGTCAAAGTGTTTAACCGGCGCTGGTTGTACCATGGTTGTAAGAATTTTATGACCTATTTGTACCTACCGTTGACCTTCTTCTTTATGCCGCTGTACTTTATCTGTTCCATTCAAGATGGGTTTCGCGGTTTGTTGGGACTGGAAACCGGAGCGCATTTCAATACTAGTGTGGACTGGTTAATCTGGTTAGCTTTAGCACTTATTATTACCACTTACTTCTTGACAATTCCACCACTGTACGCTAAGGTAGGGAACATCCAAAACATGGTGGTAAGCGCCGTGAAGTTTATTCCTTTAGTGTTTGTGCCTATCATCGGTTTTATTGTAGCGGGTACAGGTAACGGTGAATTAAAAAACGTTAAGGCATTAGTGCAACCCCCACAAATTAATGGTGCGACTGCTTCCTTTACCCAATTAGTACAAGCGGGGTATGGAATTACTCGTTTTACGGGAATTGGTGCTGGGATGGGTTCCTTTATTTCGATTGCCGCCATTTTCTTTGCGTACGATGGTTTTTACGTAACAGCTGGACTGCAGTCAGAGATGCGTGAACCGAAAAAAACACCGTGAGCTTTGTTTTTAGGTTTACTAATTACTACGCTGTTTTACCTTATCTTAGCAGTGGCACTTTCCATTAACGGTGGTTTGTTTAGTGGTATGGAAGAGTCCATGGGAAAGCTGTTTAACAATAAGCGTGCCGGGCAAATTGTCTTTGGTGTTGTTAACCTAATGATTGGCATAGGTGTCTTGGGTATTATTAACGGTTTTGCACTGTGAGCACCACGGTTTGTGGAAGACTTATTAGCCCAAGGTGATTTACCTTTTTGAAAGCAAGTGCAGGGCAGATTGAACCCCAACAAGCCAGTGGTGGGGGTAATTTACTGTTTGGTACTTTCTTTGACTGTTCAAGTGCTGTTTACGGTAATAGGTGCTTTGGCTTACCTCCCGACAGTAGCGGATTACAAAAATTACGTTAACACTGAAATTGACAAGCTCAATTCGATGCAATGGTTGTATTCGTTTTCCGATTTAATGGCTACTTGAACTTCGCTGTTTACCTTCGCCTTTATTGCCTGTGCCATCTTTGGTGCCATTGTTAATCGCAAAACCAAGCAGATTACGATTGCCAATCCAAAGCGTTATTTTCTCCCAGCGGCTTGGATTGCCGTAGTGGTTAACTGCATTAGTGTTTTTGTAACTATCATTGAGCCCTTCATTAACCTCTTTTTACTGTTTGGTTATGATGAAACAGTGGCACACACAGTGCTCGGTAATGACTTCATTGAATTGAATGAATTAGTGATAGGACGGGTAATGCTGATAGTAGTGTTAGTTTTCTTTGCCATAATTAGCTTTCTCCCTGTTTATGTGGAGGATCAATACCACAAACGTAAGTTTGGTAGTTTAGCTAACTATCAGCAATATGTACAACAACATTTAGCACACTCCACCATTAACGGTTAATTAGTTTTGTAAGGCTTGCAAGCTTTGCCGTCATTTTCATTTAAATAGCATTTATTCATTTGCATTTTTTTAGATAAATTAAAATTAATGATATAGTGTCAGCTTTGCTGTTGGCACCTTTTAAAGACAGTAAATGGATATAAATAAACCAGGTTGAAATCAGTCTGACCAACAAGCTACTGCATACGATCCCAATCAGCAGCAGTACTATGGTGATGGTAGTACTTATTACGACCCCGACCAAGCAGTTGATCCCAACCAAGCGTATTACCCCGATCCGAATACCTATCCTGATGCAGCGGCTTATTACGGCTATGGTCAGGATGGTCAAGCATATCCACAAGACTATGCTCAAGATCCCAACCAAGCGTACTATGCCGATCCCAATGCTTATCAGGACCCAAACGCTTACACCGATCCTAACGCCTATGTAGATCCCAATGCTTATCAGGACCCAAACGCTTATGTAGATCCTAATAACTACACCGATCCCAATGCTTATTACGGCTATGGTCAGGATGGTCAAGCATATCCACAAGACTATGCTCAAGATCCCAACCAAGCGTACTATGCCTATGTAGATCCCAATGCTTATCAGGACCCAAACGCTTACACCGATCCTTACTATGTTACTAGTACCGATCACAACGCTTACTATGGTCAGGTCGATAATGTACCTGCTTTAGAGGCGAGTGATCTCGCTTATGAGGTAACACCGCAAGAGCAAGCAGCGGAACAAGAGCTGTTTAGCGAACCGGAAACAAAGGTAATCCGTGAAATCCACGAATTTCCCTTTGAGAAGATTCGCTCTTACTTCCAAACGGACTTTGACAGTTATAACAGCCGTTTAACCCAGCTCAAAGACAAGTTAGATAACGCCATCTTTTCGATGCGCAAAGCGATTGATACTGTCAAGGAAAACAGCGCTAACTTGCAAATTATGAAGCAGAACTTTGAACGCCAACTCAAAGAACAGCAAACCCAACGGCTTACAAGTAATACTGACGCGGAAAAGATTGGTGCTAAGATCAACCAATTAGAGGAACGCATGCAACGTTTATCGCGCACTATGGAAAGTGTGGAGTGAACCAAAAAGGAGCCACGTCAAGAACAGTTCGATCCACGCTTTGTCGATCCACGTAACTTTAATAACTATGTAAACAACACTGACACAATGATGTCAATGTTTGAAAAGGTATTAATGATGAACTTACTGCGCAGCACCACTCCAGTCCAACCTCCAGTGCAGTACTTTACTCCTCAACCCCTTACAGCATCGCCACGTCCAGTGTATGAAGAACCGATCTCCGCTTCGTTCCGCCGTCGTGGTTACCGTGGTGATGAATTTTACGAATAAACACTGGCTCTTTTAACCGATGAATGATACTGACAAGAAGTTCCCCTTGCAACCAGTTTATGACACTGGGTTTGATGATGGGTATTTACAGCGTGATTATGAAAAGCGTTTAGAAAGTGCTGCTGCTAATGATGCACAAACAGTGGAACTACAAACCCAGCTGTTAGCAGAGATTAAAAACCTTGAAAACGAAATCAAGGCCCTAAAGGCGCAGGAATCACGCCAACCCGATCCGCACAACAATGCCCGGATTCAAAGCTTAGAAGCTAGCTTAAACCGTTTGGTCAATGAGTACAACAACTTTGAATTCCAAAAGAACTATATGGTTGATCGGGTAGCAGAGTTAAATAACAAAGCGCGTTTTTTTAAGGACGAATTAAAGCGTCTCCAACAAGAAAACGCCGCTTTTGTTAATTCCCGTTATGCCAATTGAGCTGACTTTCAATCCAACTATCAGCTCAAATTAGACCAGTTTCAAGCGTTAATTGACCAGCAAAACCAAACAATTAAGCAGCTCAACGAACAGATTGCAGCTAACCAGGGCTTAATTGATCAAAACGTACAGCGCTTACAGCAAAACCACAGTTTAGACCAACAAGAACGTGACGCCTTACTGTATGAGGTTGACCATTTATACAACGAACTGTACGAACTTGAAAACCAAAAACGCTTAGTGGGCATCGAGTATGAAGCCACATACCAGGACTTGGTGAGTGCGGATGCGGAGTTGCAAAACGTTTATGAAACAATTGCCCAAAACCAGGCCAACTTCCAAAAGCAGTGTGATGCTTATTGAGCACAGCTCAAGCAAGTAGAACAGCAAATTCAAACGACAAAGCAAGAATTGGTTGATGAAGAATCGGCACTTAAGGTGCGTTTAAATGACGCTGATTTTTACATTAACTCACGCTTAGCAGAATTAGATGATTTAACTAGCAAGATTAACGAACGTGATTTTGTTTCTAAAGAACAAGCTCAAGACGTTAAAGCTAGTTTAGCTAACCTCACTAAGGAAAAAGAACGTTTGAGCGCTGAGAAGGATAGCTTTGAAAGGTTAAGGAACACCGCTTTAAACGACATTAACCGAATGGAGCAGGAAAACGCGTTGTTTGCTAAACACTTAGAACAGCAACAATACGAGTTTGAAAGAAAGCAACAGGAAAGCTTATTAAAGCTAGAAACAGAGCATAAACAGCTCCAAAAACGAATTGGTGAGTTCAAAATCGAGAGTGAAGCTAAAAGTGAAGCGTTACTGATACAAGAACGTGAACTGTTGGAAAAACGCCGGGAAATTGATGATTTGTTAACCCAAGCCAGCTTGGAGTATGAACAACAACGCCAGACGAATCAGGTCTTAAAGGAAAAGCACCGTCAAGTCCAACAACACTTCCAAAACCTCGTGCATGCTAAAAAGAAATTGGATCAAAAGCGCCATTATTTAGCGGAACAAAAGCGGATTGATGAAGAACAGATTTTTAAGTTAAAGGAAAAGATTGCCACTGAACGCCGCGAATTGGAGAAGCTTTATCTAGTTAAAAAACAAAAGCAAGACCAAAAGGAAAACGATTTACTGATTTTTGAAAAACAGTTAAGGCAGTACCAAGCTGACTTTGAAAACGAAATAGAAGAGAAGCAAAACGAGCTGTTTGCATCCCAAAAATCACTCCAAAAATCGTTTACACAACTCAAAAATAAGGAAGCGGAATTAAACCAAAAGGCACAAAAGATTGCAGAGGATTGAGCACATCTCAAACAAAACAAACACCACCACGCTGATCTAGAAATCTTTCTGGAGGGTGAATTTAACCACTTACAACAAGAAAAGCACAAGCTGTTGGAAGCACGTACGCAGTTTGACAACCGTGTCAGTTTGCTCTCAGCACGCTTTAAACAAAAACAAGCAGAATTAGTTAAACAAAAACAAAGCTTGGAACAACTCACCGCTGCCTTTAATAAAGAGCAGGAAGCGGTAGAGCGTGATTGGAAGGATCGCTTAGCTAACTTAGAAAAGCAAAAGGAAATGCTGGGGGATAAGGTACACCAGTTTGATGAAAATTCGCTCAATATTTCCAAAAAGCTAGCGGAGCGGGAGCTAGCGATTAAATTTAAAGAAAAGGAGCTGGAGGCTGCACAAAAACAGCTAAGTTTAGACAACAACAATAACGCTGGGTTAAAGTTGCAGTTAGATAAATTGAGCGAATCACTCAAAACGGAACGCCTGGAATTAGAAGCATCTAAAGAACGTATTTTGGATTTTTACGATGAATCATCACGCCGAATTGCAGATTATGAAAGCGATTTACAAGCACGCTTAGCTGAGGTTAAGACACTAGAAAAAAACCAGCAGGAAACAGCGGCTAAAAGTGAGCGCGAACTCAAAGTAGCCTTAGAAAAGTTAAACCAAGCAAAAAAGGCTTTTTTACAAATTCGCAAACAGCAATTGCTCGAAATTGCTTCTGTAAAACAACAACTAGCACAAAAAGCTAATTTGTTGAAAAACCAACAAGCAGAGCTAGATAAGCAAACAGAAGAGTTAGAAGCAGCTTTTTTAGAGCAAGATACGGACAAAAAGGAGTTGGAAAAAGCACTCCACAGCGTTAAGTCCAAGCAAGAATTATTAGAGCGCGAACGCAGTTTTTTACTGCAAAAACAACGTGAATTTGCCGAACATGTAGCCGGCTTTAAGCGCCAAGTGCACTTTAAAACAACGCAAATGCAGCGTTTAAGTGAGTTCAACAAACAACAACAAAGTGAACAGATAAAGCGCGAAACGGAGCTCAAAATTGCTTTTGCTGATCTAAAAAAGGATTACCAGCTGTTTGAATTGCAAAAAAACCAGGAGTTCCAGCAAATCGAACAAAAGCACAAAGAACTAGAACTATTAGCACAAAAACAGGCCGAACTCAAACAGGAGTTAGAGCAAAAAGCAACAGCTTTAGCTAGCCAAGATCAAGACACAGTACAGGCCAAGCTAGATTTAGCGCGTCAACAGCATGAATTGGAATTGAAGCAAAATGCCTTTAACCAAGCTAGTCTTTCGCTCAACAAACAACGCGAACAGCTCACAAACCAAGTCAAGGTATTGCACGGTGAGTTGAAAAAACGCCATGAAAAGCTAACTTTAAAGGATCGTTTACTAGCTGAAAAGGAAAAGGATCAGCACAAAAAAGATGCAGAAATTAACCAACGCTTTAAGCAGTTTGAAAATGAATACGCTGATTTTGACCAAGCCAAAAAGCGCGAATTGCAAGAGTTAAACCAAATCCGTCGAAACTTGGAGCAGAGCAATGCATCACTGCTAAAGAAGCGCAACCAACTAACGCTTGATTTTGCTTTACTACGTAAAGTGCAACACAATACGCAAACTAACCGTGTCCAACTCAACACGCAAATTAAGGAGTTTTTACTAGAGAAGAAGAACTTTCAAAAGGCGAGTGATGAAGCCGCTTTACAAAAAGCACTGTTAATTAAACGCTTACGTAGTTTTGCTTCTAAATTACAGTTGCAACGCGAAGCTTTAGCGATTCAAAAACTGGAATTTGATAAGCGTGATGAACAACAAAAGAGTGAGATTAACAACGCTAAGTTACAGTTAGAGCAGTTCAAACTAGAAAAGCAGAACTTTGATGAAGCGAAACAAAAGCAGTTAATTGAGTTTAAGGATCAGTGCCAACGACTTGATGTTGAAAAACGTCTGTTAAAGCAAAAGTTAGTGCAGCTCAAAAACCTATCTAAGAGCTATCTTACTTACAAAAACCGTGCTGATCTGTCCCAACAACAGCTGCAACACAAGTATGCTAACTTACTGGAGTTGAAGGAAAAGCTGCAAACCGCTAAGCGTGCCTTGGACAAAAAACACCGTGCCATTTATGGCAAAATGGCACAGTTTGTCAGTGAGCTCCGCCAAGAAAAGAAGCAGCTTTTAAGTGCGCAAAAACAAGTTGATGACAAGTCACGTTTACTGGAACAAAACCAACGCCATTTACAAAACCTTTCCAGTGAAACCAAAAAGAAACGCCAGTCTTTAGAGCATGACATTAATAAGTTTGATCAAAGACGCAAGGAAGCGGTTTCTTCCATTTTAAACTCGCACAAAAAACTCAAGCAAAAAGAGGGAGAATTGCAAGGCATTTTGCAAAAGCTTAGCTTGAAAAAAACACAAATTGAACAAGAGTTTTCCAAGCTTTATCAGCAACGCGAAAAGTTAGATCGCCAACGCACTACGCTCTCTAAACTCCACAGAGAGCTAAAAGCCCAAAATGAAGCTACGGCACACAAAAATCGTGAAGTTTTAGAGATTGAGAACTATTACAAGAAGGAGTTGCAACGACTCACCACGGAAAAGAGTGAGTTTGATAACAACAAAAATCGCTTGTTTGAATACTTCCGTAAAATTCGCAATGAAATTGAAAAGAAGGAAGCACACATTAAAACCGTTTTAGAAGAAACACAAAAAAAGCGTCACCTAGTCGAAACGGAAGCGGTCAAACTACATTTACAAAAGCAATCTATCATTTCTAAGGGTCAAGAACTTAAAGAAATCAAGGAACGGGTTAGCCGTGACATTAGCCACACCAACAAACAACGCGAAGAGTTAAACAGCTTATTGCACCAAAACAAACTGTTGCAAAAGAATTTGGCTGAGCGTGAACGTGAAATTAATAATAAGGATTCACTGTTAACCCAAAAGATTCAAACAGCTAAACAAAAGTTAAGCGAAAAAGAAGCACGCATCTTAAAGCTGTTGGAAAAAATGCGTGCGGTAGAACAGCAATACCAAGCGGAAATTACCCGTTTAAAGACACGTAATGCTGATTTGGAGAAGAATGACAATAAGCACTTATTTCCACCGTTATTTAAAATTAACGGTAATGATATGAACTATCCTTACCCATATCCTTGATTTTATCCGCAACAAAAACAAGAAGATAGTTCAAACCAAATTCGCCATCTTTTTGAACAACAGTTGCAATTTATGCAACAGCGTTACGAAAACGAATTAACTGAGTTGCGTCGCCAACGTGCTTTATTGGAAAAAAACTAGACCAAATTCAACTCGAATCACAGTTAAGCGCGAAGAAGAACGACTTTGAAAAGGTTGAGCAAATGATGCAAAAGTTGTTGGAAAAAACTGAGCAAAAACTCAGTGCCTTTGACCAAAAAATTAATGCTTTGGCAGAACAGATTAACACACAAAAAGCAGAGCACGCTGACAGTGAAAAGCAACAGTTGTTACTAAGGATTGAACAGTTAGAAAAGCAAAACCTAGCTCAAGCTGTTCAAACACCCCAACCAGTACAACCAGTGGTACAAGCACCAGCTGTAGTACCCCAAGTGATCCAACCACAGGTAGTGCAGTCCCAACCAGCTTTTTTAGCAACGCAGCAAAGTATTTCTAAACAGCAACAAATCGCGCAATTAAACGCTGAGATCAATAGTATTAAAAAGCTAATTGCCCAAAAAGCAGCTAAATAAGGAAGAGATGAAAGATGACTAATGATTACCAACAATTAAATTATTTAGTGGAAACTGATGATGAAGCTGACATCATCATAGCTAACCTCGTCAAACAACTCAATGAATTGAAGGAAATTCTGCTTTCATTGGACAATCAAGATTTAGAAATTAATCAGGTTAACCATCGCACCACAGTGCACAATACTAGTTCTAACACTTCTAATAATAGCACTAGCAATAATAGTGCTGATGGTAATTACAACAGCAATTTCTTTCACAACTTTAGTAAAGAAACGCTGCAGACACAAGCAAAACGTGGCTTTCTTTTACTAGAACGCTGTTCCTTGGTAGGTCTACAACAATTGGAGTTGGAATACCTCAACGTATTGGGTCGTAGCTTTGAATCACATCAAGACAAAATTAACTTTTTGGTCAATTTACGTGATTTAACTAACGATCACTTGTTGGACACGGAAAAGATTGTCAGTACCCTAGAGCAGATCTTTAACGTTATTGGTGGCACGGAATACACCCCGATTCTCAATTCCTTTTTTAACCAATCACTTAACGATCCTGACCCAATTCAGCGCGAAATTGGACTCAAACAGTTTGTGGCTAACTTACGCCAACGCTTTAAAACGCTATTGCAAAAAACTAACAACTCAATTCAACAGTTAGAAGCGGAAATTCAAATTCCAACTACTCATATTAAGAGTGATGAGGTAATGTTTGGCCCACCGGACATGAACGAACGCTTAGTGTTAAATGACAGTGAAACCGATGCCATTCTACGCTCAATTGAAGCGGAGTTGGAAAGCGCTTTACAAAATAAAAAGCAGGTAGTGGTTACAGCAGTCCCACCAATAGCTGCTAACTTAGCTACCGATAGTATTAGCCAAGAAACTTTAGAACACAACCTTAATAACACTGAAACGGTTAACACCACTACTGTCACGGTAACATCAGCTAGTGAAACTCAAGTGCGTAAACCACAGATTTCATTACGCCCCATTTTTCAAGGCAACTTTCCCAAACGCTTAAGTAGAGAAGACATCCAGCGCTATGCCCACCAGTTACAGGAATTAGAACAAAGTAATGAAGGATAGTGCACTAACACTAAAACGGGTACGAATTGGAAAATTTTCCGAGTCAATGGTTGAAGAACGACCAACACTCAACCTGTTCGAAAAGGTCGAATTCAACCCAGTACCGACTGCTTTAGTGGATCAATTGCCAACTGAACCACTTGTTGAAGCAACTCTTTTAGAAAAGGAAGCAATTACTTTTGTCGATACTTACGCAACTTCGGAAGCTCATGATCAAATAGCTACCTTTGTTTTAGAACAATCAATGGAAACGGAGGTGGTTGAAAAAGAAGCGATTGAAACAGCAATAGTTGCACCAGCACCAGACTTAGTTGAAGAGAAAGCTGTTTTAGTGGAAGAGGTTTTAGTTGAACCAACTGCAACGGAAGCAGTTACAACTGAAGAAAATCAAGTTTCAACTACATCCGTAACTAAGATTAAAACTAAGCGCAGCAATACTAAAAAGGTGACATCAGAAACACTTGTGGCTTCTAAAAGTGTGAAAACTAAAAAGTTAATTACCCCTAACCGGGTAAGTTCGGGCAACGTTAATATAACACTTTGACAAGCAGACAAGAAGTCCACTAACCTAACTAAAACTAAAACCGATCTCTTTGGTAAAAAGCACCAGTTTAAGGGTCCACAGTTAATTTCTTACAAGAAGTAAGCAAAACAAAAGCCAGGCACTAACCTGGCTGTTTGTTTCGGAGAATCTAAACAAGTATTTAAATTTAAGTATTAAGTATTAATTAAACGGGAAAAGAATAGGACGATTTACCAGGGGTTAAACCTAATCGGAACCGACCGGTACTAAAGTAGTCTTTTTGTGAATTCCTTCTTTTATAGCTATAACTGCTGCCATTGCCGTTGGCATTATTAGCGTTAGCAGCTTCGCTGATGTCTAACGCGGTATTAATTAACGTACTAAAGGCGTTTACAATCATAATTACTCCCGCACTGAAGCTTCAAAATGATAAGCCTCCAACTACTTGTTTAGCTTCTGATTTTTTTAATCTCTGCATTATTGTGTCTCCATTTAAATGAATTACATAACTAAAGTAAAATGCCAAAGTTTTATAATTAACTTAACTGTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCATAATACTTTCTAACCTACCAGTGTTACCCTGGTAGGTTTTTTATTTGCTCCGTTGGCTCACGGTGGATGAAACCACCTTTGACTTACTTTTGTAAACCCAAAAAGTGGTCTTGTAAACCACTTTGGTGCCTTTTACTTTGCTGTTCCAAAGCGCTTTTTTTAGGTTTGCTGGCAGTTCTTTTAGCCAAAAAAGTTTTTTATTCTAAGATATAAAGTGTTTTAAAGTGTCACAAAGTGGCATAAAGTGGTAAAAATGCTTTTAGGAACCTTCAACATTACCCTTGATGCAAAAAACAGGATCAGTCTGCCAGCGAAGCTGCGGGCTTTCTTTGAAGGTAGCATTGTTATCAACCGTGGCTTTGAAAACTGCTTGGAAGTGCGTAAACCACAAGACTTCCAAAAGTACTTTGAGCAGTTCAACAGCTTTCCCTCCACCCAAAAAGACACTAGAACACTCAAGCGCTTAATTTTTGCCAACGCTAACTTTGTGGATGTAGATACAGCGGGACGGGTTTTAATTCCCAACAATCTGATTAACGATGCCAAGCTAGACAAGGAAATTGTTTTAATTGGCCAGTTTGACCACTTAGAGATTTGGGACAAGAAGCTGTATGAGGACTACCTAGCCAACTCTGAGTCGTTGGAAACGGTAGCGGAAAGGATGAAAGATGTTAAGTAATCAACCCCACAAAAGTGTCTTACTTGATGAAGTCATCCACAACCTCAACATTAAAGGTGATGGTAACTATTTAGATCTTACTGTTGGTTTTGGCGGTCATAGTCAGCACATTTTAGAGCAGCTTACCACTGGCACTTTAACTGGCAATGACATGGATAAGGACAGCATTACCTTTTGTACCGAGCTGTTCAAAGACAAAAAGAACGTTGTTTTAGTCCATGATAACTTCGCTAACTTTTTTAACCACTTAAAGCAGCTCAAAGTAACGAAGTTTGACGGCATCTTAATTGATTTAGGGGTTTCGAGCTACCAGCTAGATAAACCAGAACGCGGTTTTAGTTTTAAACACGCCGGTCCATTTGACATGCGCATGCACCAAAGCGATCGCGTACCAACTGCTTTAGATATCTTGGAGAGGTTGTCTGAGGAAGAATTGACACATGTTTTAAAGAAGTTTGGTGAAATAGCCCACCCCAAACCAATCGCCAAGGCCTTAAAAACACTCATTAATAACATTAAAAATCCAACTACGGTAGAGGTAGCGGAAACAGTTAAGGCAGCTGCTAGCAACTTTGAAAAGTACAAATCACGCAATTACTTAGCTAAAGTTTTCCAAGCCATTCGCATTTACCTCAACCGTGAACTGGAAAGTTTGGAAATTGTGCTGCAACACATTCCCAAGCTGTTGAACAATAAGGGTCGTTTTCTAGTGATTGTCTTCCATTCACTCGAGGAAAAATTGGTGCGGAACTACATTTTTAAACTTACTCACTTTGTCCAACCCCCTGAACTACCCGTTAAATTAACCCCACCGTTTGAACTCATTACTAAAAAACCCATTTTGCCAACTGAACAAGAAATTAAAACGAACCCCCGTGCTCGAAGTGCCAAACTGTTTGTCATTGAAAAGAAATAACTATGTATAACCTCAAAAACATCTACGCCTCCATTACCCTTTACCCCCATGAAATTAACTTGGTGGTATCGGACAACAACAATCAGTTTCATGTCTTGTATGAAAACTCGATTGCTAACGATGAGCTTTACACGCACGCGGGCATTACTAATAAGGCAAAGTTCAAGGTAGTTTTAAACCAGCTCATTAACAATGCAAACGATTATTTGGGTTTTAAACTCGAAAAAGTAATTGTGGTTTTAGCGGAGTTAGTTGATGATCTTGAAATTAACCGCTTTAAAACAGATGTCTTTTTCACTGGTTATGACTTTAACCACCAGGACATGTTGAGCAGTGAAAAGAACCGCTTCTTGCTCAAAAACCGTCATATCAACCCCAATGAAGTTTTGGACTTAGTGGCTTTAAACTACCGTGATTTAACCACTGGTAAAATTAGCAAAAACTTTAAGTACAACTGCAGTTATCGGGCAAATGTAATTTATTACACCACTAAAAACTCACTAGTCAAGGAGCTCAAACCGTTTTTAAAGCGCAATATTATTGTCAAAATTGACAAAATTGTCACCCACCACATGGTGTTAGCCCACTCAATTAAACAAATCAACAAAAACAACCTCTTTGTGTATTTAGGGGAACACACTACTGACCTCATGTTGTTTATGGACAATGCCTTAGTTGATTTAGTAAGTGAACCCTTTGGTCGGGTTAACTTCTTGGAATCTGACAATGACAGTGAAAACAAAGCGCTGTTGGAATTCCTAGTGGACTCTACTGCACGCATTGGTGACTCGGGCTCGATTGGCATGGCTTACACCGATGGTTCTACTTATAAGGAAATTAGAGCTGTTACGATTGATGACCTCATTCAAAGTGTTAATGAAAAAACGAAGTACTTAATTGATTTCATTAACACTAACACGGAAATCTTCTTCCAAAAGTACGGCTTTTTACCCAGCACTTTACTGTTTTACACTAAGAGTAAACAGTTGCTTAACAACTTTCAACTCAACCAAAACCTGTTGAGTAACCACTTCAAAACTGTAACCATCTTTAAAAACGAAGTTCAGTTTGTGAGTCGCAAATACTTATTGAACTGTCAAGCAATTGCGCTTTCTTGAAGACAAGACCAAATTAATGTTAATCCCACTAGCTTTGCCCTACCGTTTTACTCAGACAAAATTGAAAAGCAGTTTAAAAAGTCGTTTTTAATGTTGAAGGTACACACGCAAATTAATAAGTTAGTGCAAAAACTAATAAAGTAAGCTTTATGGATTGAATACAAACAGCAGGTGCTGGTACTCAACTACCAGAGAACAACATTAAGATAGCCGTTTTTGGAATTGGTGGTGCTGGTAACAACATTATTGATGACATGTTGCGCATGCACCCCGAATTGCAAACGGCTAACGTCCAGTTCTTTGCTTTAAACACTGATTTACAGCACTTAAAAACAAAGCGCTATGTCCAAAATAAGGCTGTGATTCAGTTTGAAGAATCCAAGGGCTTAGGTGTTGGTGGTGATCCCCAAAAAGGTGCCGTTTTAGCACACCATTTTTTAGAGCAATTTCACAAACTTAGTGACAGCTTTGATTTCTGTATTTTAGTCGCTGGTTTTGGTAAGGGGACTGGTACTGGTGCTACCCCGGTTTTTAGTAAGTTTCTTAGCAACAAAGGGGTGTTAAATCTCTCGATTGTCAGTTACCCCGCAATGTGTGAAGGGTTAAAGGCACGCGAAAAAGCAGCTAAAGGTTTAGAACGCTTAAACCAAGCGACTGACAGTTTTATGCTGTTCCGCAATGATCGCTGTACTGATGGCATTTACCAACTAGCAAACGTTGCCATTGTCAAAACAATTAAAAACATTATTGAACTAATTAATCTACCACTACAGCAAAACATTGATTTTGAAGACATTCGTTCTTTCTTTAAAAAACCAGCACAACGTTTAGAGAATGAAGCTAACTTGTTTCGCGTAACTAATACTTTTACTTTTAGCTTTAGACGCGCACAATACAATTGAGCACTTTAGCCACAAATTGAAAAACTTTGAGTATGAAGGTTTTTTTGACCATAAGGTAGAAGGGGCGCAAAAGGTAATTTTAAAGGTGTTAGTCAACCAAGGTTTATATCCGCTTGATTTAACCCAAATTCAAGAGATTATTTGAGCCAAAATTGACAATCATAACCTAGAGGTACAACTTGGAGTTGACTTTACTGATGCCAACCCTTCAGTGCAACTGTTTTTCTTAATGGAGAAAAAGCAAGCAGTGTCCTCTGACTTTATTCAAAAACCAGCATTTATCTCAGTTAAAGAAGTAAATCAGAAACCGGCCAAACCGTTCCAAGTTTTGAATGATTTAAAGGAGCTGGGACTGAAATACGTCAAACAGCAAACCGGATTTAATTATTAGTGGTCTTTCTTCTTTAGAATAAGCTAACTAATAAGAAGGTTAATGGCTGTTGCAAGTAAGAAGTTCACTGAACGTCAATTTTTATTTTTTGTTGTCAACTATATTGCTGGCTTTGGTTTTATTTCCACTGCCCTATCAATGTTTGAGCTAGGACCACTTTCATGAGTGATCTTTTTTCTCGTTAGCTTAATTAGCCTCATTGTTACCTTAGCATTTGCCAGGGTATCAGCGATTGACAAAAATAATTACGGCGGCGCTTACCTGTGAGCCAAAAAAGCCTTTAACAGTGAACGGTATTCCCACCGCTTGTTTATCTTCTTTACCGGGTGAAACAATTTTATTGCTTCACCGCTATACGCCGCTATTGCCCCGCTTTTTATTCTCTCTGCTTTTAAAGGTATTGATGGTATTCAGGGCAATGCAGCTAACACTTGAATCCTTATTGCCGCTGGTTTTACCTTTTATTTACTGTTAGCCTTTCTTTCCACAAAGGGAACTTCCCTCAATAAAAAGTTAATTGCGGTATTTGCCTCAATTAAATGGGCGGTGCTTTTGAGCACCTTAATAGTAGCACTTATAGTTATCGGCAAAAATGGTAATGGTTATGCCATTAACAACGACATAAAACAAGGCCCTTTTAAAGAACGTGAAATTACCTTGGGTGCAGTTGCTACTGTCTTTATTACCTTCTTTTATTCCTACAGTGGTGCGGAGGACATTTCAACAATGACACCGGATGTGAAAACCACTAACTTCCGCAAGATTTTAATCATCTCTTTTATTGCGGTATTCTTGTTTTACTTTGTGGGCATTTTGATCTTTAATGGTTTATTGAATGTTTCACAAAGCGATGATGATAAGGGGAAAGATGGAAGAATAGCTAGTGTAGCTGATATTTTTAGATTAGCTGGCGGTTTAGGGGCACTCATTTTTTACGGTGTGGGTGCTTTATTCAATAACGTATCTACCAGACTTTCGACCATTATTGCTAACTCACGCAAGATCTTACCGTTAGCACAAGATAACTATTTACCCACTACCTTTAGCAAAGCTAACAGTAAGGGCGAGTTCCAAAATGCAATTTGGTTTACCTTTGGTACTACCATCATTACGATGACGGTACTGTTCTTTGTTCCGCTAATAACCAGCAACTTTGACTTCGACAAATCAACCGAGTATGCCGCCTCGATAGGTTCGGCCGCTACACTGGTACAATATATCCTTGTATTTTTTATAATTTTTAGGCTTATTTATAAAAAAGAGCAACTTTATCAAAAAAACTGGACACGCGAGTTAGAACAAATACTGTTTGGGATTGGTGTCTTTATTATTCTTCTCATGTTGCTCTTTTATCTCTTTCCAGTAATTGATGGAAAAACTAAATGGCAATTGAAAAATACCCTCACTTTAGTGGTGTATGGGGTGTTGATTTTAATTGGATTTGCCCTCTTTATGACCCAAGAATATAAGCGCAATAAACAAAAGGAAAGCTATGTCACACCAAGAGCAGTTACATAAACCTAACCGGCAACAGTTTAGCGAAAAGCAATTTATTGCTTTTGCTTTTAACTATGTTGCTGGGTTCGGCTTTATTTCCGTGGTGCTAACCATGTTTAAGCTTGGCCCGTTTTCTTACTTGATCCTAGGGCTAGCAGCTTTAGGTATTTTGGGGGTAATGTTGTCCTTTTCGCGCCTTTCAATTATTTGTGGGAGCAAGGCTTACGGTGGTAGTTATTTAATAGCCAAAAAAGCACTTGGTGCGAAAACAATCACGGCGCGTTTCTTTACTTTTTTGAGTGGTTGAAATGTTTCCTTAACCGGTCCTTTTAATGGGTTAATTGTGCCAGCGGTGCTAGTTCTCTCGTTTGCTGATATTAAAGCAGTGAAGGATAACAATGGTGCTTTAATTGGCTTACTGGTCGGGGGCTTTGTTTTATTTGGGGCATTAAACTTCATCTCCCTGTTTGGCCTTAAAATGAATAAGAACGCCATTCTCTTCTTTGCCATAGTGAAGTGAGTAGTTGTTCTGGGCGGTTTAATTTTGGGAATCTACCTCATCGGTACGAACCACGGCCATGGTTTTGTCGAAAACAACACTTTAGGTGAACACATTGAAGATCTCAGTTTCCTTAAAGTTATTTCTACTACTGTGGGGATGTTGGTGGCCTTTGCTGGTACCGAAGATCTTACTGCGATTACCCCGGATGTGAAGTCAAAAAACATCCGTAAGTGTTTCTTATTGATGTTTGGTGCAGTTACCCTGCTTTATCTAATCGGTTTTGTCATTATTAGTGGGATTTCTGGTTTAAATGGTTATGGCCTTGATGGTAAAGAAAAGAATGAAAAAGCGATTAACACCTTTGGTTCGATTTACTTTCAAGCAGGCGGTAAGTATTTAGGCATCCCACTGTTGGTCATCTTTGGTCTGGGTTTTTTACTCAATTCCTTGGCATCACGATTAGGCATGACCATTACGACAGCGCGCAAATACGTTGCGTTAGCCCAAGATGGTTTCTTGCCGTCCTTTATTAACGAACAAAACAAGCACCACGAGTACCATAAGGCAGTGTGAGCTAGCAACATTATGACCTTAGCAGTGATGGTGCTGATGATCATTGTCCCCTTTTTACCTAATGATGAAAACCCCGGTAAACAGCTCGTTATGTTCGATGCCATTAGTGTTTTAGTTGAAGTGGCGATTGAACTAGCAGTGTTAATTTCCTTAATCCAGTACTTTATTACCTACATCTTCTTCTTTATGATTCTGGCCAAAAAAGAGGGTAGTGCCAGTGTTTCGTGATGGGAAATTGCCAGTTATGGCGTAAGCTTTGCCATTATTACGGTTTTGTTGTTTGTTAACCTCTTTCCTATAACGGCTTGAAAGAACACCAACACCTTTAAGCTAAGTATCCTAGCGGCCTTCTTTGCCTTGGGTATTGGCTTCTTTATCCACTCGGAAATAAAACACAAAGGACAATTAGTCAAGTCAGTCGAGTGTAATTAAGATCACTCCATTAAACCGGTAGAAAAAGCCGTCACGCATTTCGGGTGGCGGTTTTTTCTTGTTCTTTTGACTGCCATAAGACAAAAAATACAAAAAGCATTCAGCGTTTATCTCTTAACCACCGGTTTGAATAGTTAATTGAGCTGCTAAAACGGCTTTTTAGTGTCTTATAAGCGCTTAGAAAGCCTTTTCTGTGTCATTTTCCTAGCTTGAGCCTAAAATGAGCCCTACAGGCCGTTTTGGGGGCTTACAAGCGGTTATTAACATTGTTTTAACAGGTTATTAACATTGTTTTAAAAGGCTCCATTAGCTCATTTTTGCAGTGTTAAAACCGCTTTAAAGCTTATAAAGCTTTAAAAAAAGGCGTTTAAATGAGCTTTAAAATAACCGTTTTAAGTTATTGCAAAAACTCTTTTAAAAAAGTTATTCGCTTATTTTGCAAGCTTAAAAAAAGATTTTTATACAATGCTACACCATCGAAGTTTAGGAGAGTTATGCAGCAGTATCTAGATTTAGCCCGTTATGTTTTAGAGCACGGAAAATACCGTCCAAATCGCACTGATACTGCTGGCATTGGTGTCTTTGGTTACCAGATGCACTTTGATATTTCAAAACACTTTCCGCTCTTGACTACGAAGAAGGTGCACTGAAAGTCGATTGTGCACGAACTTTTGTGGTTCATTAAGGGTGACACCAATATTAAGTACCTAGTCGACAATAAGGTCAACATTTGGAATGAATGACCTTACGAAAGCTTTAAAAAGTCACCTCACTTTAACGGTGAGAGTCAAAAGGAGTTCATTGAACGGATTCGACAAGACGCGAAGTTTGCCCAACAGTTTGGTAACCTCGGACCCGTTTATGGAAAGCAATGGCGTGACTTTAACGGAGTCGATCAATTGAAAAAGGTAATTGCGCAAATTAAGGTAAATCCGTTTTCGCGGCGCTTAATTGTGTCTAGTTGAAATCCCAATGAGGTGGATCAAATGTTGCTCCCGCCTTGTCATTCACTGTACCAGTTTTACGTGCAGGACGGGCAGTTGAGCTGCCAGTTGTACCAGCGCAGTGGTGATATTTTTCTAGGTATCCCCTTCAACATTGCGTCCTACTCTCTTCTGGTGTACTTAGTTGCTAAGGAAACTAACCTAAAACCAGGGAGCTTTGTCCACACCATTGGCGATGCCCACATTTACGAAAATCACTTGGAACAAATTAAGTTGCAATTAACACGTCAACCCAAACCATTGCCCAAGGTAGTTTTAAAGAGTGATAAATCAATTTTTGATTATCAATTCGATGATATTGAACTGGTGGATTACGACCACCACCCCACAATTAAAGGAGAGGTAGCAGTTTAAGATGGTAAAAGCAATTTGAGCAATGGATCAAAATGGGTTAATTGGTAACGGTAACAGTTTGCCCTGAAGGATTAAAGCGGAGTTACAGCACTTTCGCCAGACCACGTTGCACCAAGATGTATTAATGGGCTCGGCTACCTATCTTTCCTTACCGCCGGTGTTTAGTGAGCGTAACGTTTATATCCTCACCCGTAACCTCAACTTTAACCCGCCTGATAAGGGCTGTTTGACTAAAGTAATCCATGAATATGAAAACTTTATCCAACCTTATTTGCACCATCCCGATAAACACCTCTACATCTGTGGTGGGGCACAAGTTTATGAACAGCTCATTCCCCGGTGTGATGCGTTAATTGTCTCTACCATTTTCGGTAAATACACCGGGGACAAATACCTCAAAGTGGATTTTAGTCCCTTTGAATTGACCAAAGAGATCAGTTTTGCTGAGTTTAAGGTCGCTTACTATCACAAAATTGCACGATAAAACAATATGGCTAACACAAAAAAATACTTTTTAGAATCAGTTTCTCCCTTAGAATATGCACAAAAAAAGTTTCAGGGCAACTTGCGCTCGGTTAACTGAAACCTAGTGGATGATGAGAAGGATCTGGAAGTGTGAAACCGGATCACGCAAAACTTCTGGTTACCAGAAAAGATTCCGGTATCCAATGACATTCCATCATGAAAGCAGCTGTCTAAGGAATGGCAGGATTTAATTACCAAAACCTTTACGGGGTTAACATTACTTGACACCATCCAAGCTACCATTGGTGACATTAAGCAAATTGACTATGCTTTAACAGATCACGAGCAGGTAATTTACGCCAACTTTGCCTTTATGGTGGGGGTACACGCGCGCTCTTATGGCACCATCTTTTCCACTCTGTGTACTTCAGAACAAATTACTGAAGCACATGAATGGGTGGTGAAAACCGAAAGCTTGCAAAAGCGCGCTAAGGCTTTAATTCCCTATTACACCGGGAAAGACCCATTAAAGTCTAAGGTAGCAGCCGCTTTAATGCCCGGGTTCTTACTGTATGGTGGGTTTTATTTACCGTTTTACCTCTCCTCGCGTAAACAGCTACCCAACACTTCGGATATTATTCGCTTAATTTTGCGCGATAAGGTAATCCATAACTATTACAGTGGGTATAAGTTCCAGCGTAAAGTGGAAAAGATGTCCAAGGAAAAACAGGCTGAGATGAAACGGTTTGTCTTTGATTTAATGTACGAACTGATTGAGTTGGAAAAGGCATACCTCAAGGAACTGTATGAAGGGTTTGGGATTGTAGAAGATGCCATTAAATTCAGTATTTATAACGCTGGTAAGTTTTTGCAAAACTTAGGGTATGACTCACCGTTTACAGAAGAAGAAACGCGGATTAAACCAGAAATTTTTGCCCAATTGTCAGCCAGAGCCGATGAAAACCACGATTTCTTTTCTGGTAATGGTTCGTCATATGTGATGGGAATTAGTGAAGAGACCGAAGATAAGGATTGAGACTTTTAACAATGCATAAAGATATCAAAATAGTCGACGCTAGTGCGATCGTTAAACCAACTGGAACACCGTATGTAGTGTATTTTTCCTCCATTTCTAATAACACCCACCGTTTTATTGAAAAATTGGGGTTTGAACACACCCGCATTCCGGTTAATTTAGATGAACAGATTGAAGTAAACCAGGAGTATGTGCTCTTTTGCCCCACTTACAGTGGTGGTGGGGAATACACATCGGGGGCGGTACCCAAACAGGTAATCCACTTTTTAAACAACAAGCACAACCGTGATTTGTGTCGTGGCGTAATTTCGTCCGGGAACACTAATTTCGGCAATACTTTTGCCATTGCCGGGCCGATTTTGTCGAAAAAGTTAAACGTACCACTTTTATACCAGTTTGAACTTTTAGGCACGAAAAACGATGTAGAACAAGTTCAAACCATTATTACCAATTTCTTTGGTAAAGCCAAATAACCGTTATTAATGATTAGCCAATTATGTCAGTTAAAGAAAAGATTCCTGCTTTCAACACCCAAGAAGACTTAGAAAGCTATATTTCCTTAAATGCCTACACTAAGGTGTACGGTGATTTTAAGATGGATTTACACGCTGTGGAGGCTTACATCCAAGAGCATGTCAAACCCAAAACAAAGGTGTTTCACTCCACCAAGGAACGCTTAGACTTTCTAGTAAAGAATGATTATTACGATGAGAACATCATCAACATGTACAGCTTTGAGCAGTTTGAGGAAATTACACGTAAGGCTTACGCTTACCGTTTTCGTTATGCTAACTTTATGGGGGCATTTAAGTTTTACAACGCTTATGCACTGAAAACCTTTGATGGGAAGTGGTATTTAGAAAACTATGAAGACCGCGTTGTCATGAACGTGCTCTTTTTAGCCAATGGTAACTATAACAAGGCTTTAAAGTTACTCAAACAAATTATTACCAACCGCTTTCAACCAGCTACCCCAACTTTTTTAAATGCGGGACGAAAAAAACGTGGTGAGTTTGTGTCGTGTTACTTACTGCGCATAGAAGACAACATGGAATCAATTGGTCGTGCCATTACCACTACCCTACAGCTCTCCAAGCGTGATGGTGGGGTGGCGTTATTGCTCACTAACATCCGCGAATCGGGTGCGCCAATTAAAAAAATAGAAAACCAGTCATCAGACATTATTCCAATTATGAAGCTGTTGGAAGACTCTTTTTCTTACGCCAACCAATTAGGGCAAAGACAGGGAGCAGGAGCAGTGTATCTCCATGCCCACCACCCCGATGTCATGCAGTTTTTAGACACAAAGCGCGAAAACGCTGATGAAAAGATCCGGATTAAGTCGCTGTCTTTGGGTCTAGTTATTCCCGACATTACCTTTACTTTAGCTAAAAACAATGAGGAGATGGCCTTGTTTAGTCCGTATGATGTGTATGAGGAGTATGGCAAACCCTTGTCAGACATTTCAGTCACGGAAATGTATTACGAACTGTTAGCGAACCAACGCATTAAAAAGACCTTTATTAATGCGCGCAAGTTCTTCCAAACAGTGGCGGAACTGCACTTTGAAAGCGGTTATCCTTACATCTTATTTGACGATACGGTTAACCGGCGCAACGCGCACCCCAACCGCATTGTCATGTCTAACCTCTGTAGTGAAATTGTCCAACCCTCCACTCCATCGGAGTTCCACCACGATTTGGCTTTTAAAAAGGTTGGTAATGACATTAGCTGTAACTTGGGTAGCTTAAACATTGCCAAAGCAATGGAAAGCGGACCGGAGTTCTCGGAGTTGGTTAAACTAGCGATCGAATCACTTGATTTAGTTTCAAGGGTAAGCAATTTAGAAACGGCTCCATCGATCCAAAAGGGTAATTCCGAAAATCATGCTTTGGGATTAGGGGCAATGAACTTGCACGGTTTTTTAGCAACTAACCAAATTTATTACAACTCCCCTGAAGCAATCGACTTTACTAACATCTTTTTCTATACGGTAGCTTACCATGCCTTTAAAGCATCCAGTGAGCTAGCTTTAGAGAAGGGTAAGTTTAAAAACTTTGAAAACACCAAGTTTGCTGATGGGAGCTACTTTGATAAATATATCAAAGTAGAACCAGACTTTTGAACACCCAAAACCGAGCGCGTTAAGGCGTTGTTTCAAAAGTACCAAGTGGAAATACCAACACGTGAAAACTGAAAGGAGTTAGCACTCAACATTCAGAAAAACGGTTTGGCCAATTCCCACTTACTGGCAATTGCTCCAACGGGTTCAATTTCCTATCTCTCCTCCTGTACTCCTTCACTCCAACCGGTAGTATCCCCGGTAGAAGTGCGCAAGGAAGGTCGGTTGGGACGGATTTATGTACCCGCTTACCAACTCAATAAAGACAGTTACCCTTTTTATAAAGATGGTGCTTACGAACTGGGTCCCGAACCAATTATTAACATAGCAGCAGCGGCACAACAACACGTCGATCAAGCGATTTCGCTCACCCTATTTATGACTGATAAGGCTACCACTCGTGACCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTTCTTCCATTTACTATGTCCGAGTGCGCCAGGAAGTACTAGAGGACAGTGAGGATCACACCATTCAAATGCAGCAGTGTGAGGCCTGTGTGATTTAATAATACATATAAATACATATAGTAAGTCTAGCTTTAAGCATGACTTTAGCAGGGGTTAATTTGTTATAATTTCACGCTTATTTGCGTCTTCTGCGGTTCCTAAATTTTGTTTATACAAAATACCAAAAGAAACGGTTTTATTGAAAATGCATGCTATTGTAGTCTGTGGATCAAAGCAATATTTAGTCCACGAAAATGACACTTTTTTTGTTGAAAAACTTGAAGCGCCAGTTGGTAAAGAAATCCAACTCGACAAGGTTTTAATGTTGGATGAAAAGATTGGTGCGCCATACTTGGAAAAAGCGCGAGTAGTGTGTGTGGTGGAAAAGCACGGTTTACAACGCAAAGTAAACGTGATTAAACACATCTCACAAAAACACCACTTAAAAAAGTATGGTCACCGTCAACCTTACACTAAGTTAAAGGTAGTGCGCTTTGTCCATGATTAAACTAACTGTGTCGCACCACAAACTCGCCGCTTCCGGGCACGCTTTATTTGCCAAAAAGGGGCAAGACATTGTGTGCGCTGCTGTTAGTGGTATTATCTTTGGCGCTTTGCCCTGGTTTGAAACCAACAGCATTGCCGTTCAAGAAGATGCTACTGTACCCAGTTTAAGCTTGGAGTTAGTGCAACCAACCGCTAAGCTTATCACAGGATTGAGTGTGGTGATTATGCAACTTAAAACCCTCGCGCATTCTTACCCGCAGTTTATTTCGTTTGAAGATCAACGTAAAGATGAATAACAAATATTTTTTAACCAAAATTGACTTACAGTTTTTTGCTTCCAAAAAGGGAGTTGGTTCAACCAAGAACGGCCGTGATTCTCATGCTAAGCGTTTAGGTGCTAAGAAGGCTGATGGTCAGATGATCCGTACTGGTCAAATTATTTACCGCCAACGCGGTACTAGGGTATGTCCCGGTGTAAATGTTGGCCTTGGTAGTGATGACACCTTGTTTGCTTTAAGCGACGGCTTGGTAAAATACCAAAAGTTTGGACCTAAACAGGGCAAGACCCGCGTGAGTGTGGTCAAACACAAACTAGATGCCTAAACTCCTAGGAAGCTTTATTTCGTTTAAATCACCCCATTATCTAGTGGGGAGTGTTCGTGACGCTGTTAGCATTAAAGCACAAGCGTTCATGATTTTTTTGGGCGCACCACACACGGCACTACGAGTCGATCCTAATCGTATGCAAATTGACGAGGGTCACACATTGATGGAACAACATAACCTCAGTAAGTCTGGGATGGTTGTCCATGCTCCTTACATTATTAACTGTGCCTCCAAAGATCCGGTAAAGCAAACCTTTGCGATTGACGTTCTGACAAGGGAGGTTAAACTGTGTCATGCCGTGGGTGCTAAACTAATCGTTCTCCACCCCGGTTCAGCGGTTGAGCAAACTCAAACCCAAGCGTTAGATCACCTAATCAAGGTCTTAAATACAGTGATTGCCAACACTAAAGAAGTCATTATTTGTCTCGAAACGATGGCTGGTAAAGGTAATGAAATTGGCCGTGATTTAGACCAGTTAAAGTATGTGATTAACCACATTGAACAACAAGAGCGGATTGGTGTTTGTCTAGATACCTGTCACTTCCATGACAGTGGTAATGACTTTAACAATACTGCCGAAATTATGGAAACGATTGACACTAAACTAGGCTTTGAGTTTTTAAAGGTCATCCACTTAAACGAGTCCAAAAACGTTTGTGGTTCCAAAAAAGACCGTCATGCCAATCTAGGTGAGGGGATGATTGGGTTTGATAATTTAATGCGCTTTATTGCCCAACCGCAAATAAAGCAAATACCGATTGTTTTAGAAACCCCATCTGACAAGCATAATTATCCCGCTGTATATGGTGCTGAAATCGAAAGGATCCGTGCTTGATTTGGAGCACGATAACCAAACACTAAAGGATTACATTAGTGTGTTTGAACGCAATAAGATGCGTTTAACCCAATCACGCTTAATGTTGCTCCAATGCTTAGTGCAACACCGTGACTGGCACACCTTAGCTGAGCTCAAACACCACTTGGAACAAAACCAACAGCGCACTACCTTAGCTTCTATCTATAACAACCTCAAGATTTTTGCTAAGTTAAAGCTAATTAATATTTTTGTAGATACCAATCGCTTTGAAACGTATTATTGCTTAAGACACGAAAACCACAAGCACATTTACTTCTTTGACGAAAACCAGCGCAAGTTTTTAACCCTACCACTACAAGACAAGGAAGCGTTGAGTTTAATTGGACATAAAAGTAAACATGGCAAAATTAAACTGAATGACTTTTACATAGTCGCTAGCGGTACACTTGAAGATGATCAATAAGCCAAATCAGTTTGTCAACCACTTAAGTGCTTTAAAAAAACACTTTGCCTCTTATAAGGAGCTCCGTGAAGCGTTTAACGATTATCACAAGCATAACGGTGATGAGTTGACCACCTTCTTTTTGCACCAGTTTGATAAGGTGATGGAACTAGTCAAGCAGAAGGACTTTAAAACTGCCCAGTCTCGCTGTGAGGAGGAATTAGCCGCCCCTTATCTACCAAAACCTTTAGTAAGCTTTTTCCAATCGCTACTCCAGCTCGTGAACCATGATTTATTGGAACAGCAAAACGCGGCCTTGGCTAGTTTACCCGCAGCCAAAATCATAGAGCTTGTTTTACAGGATTACCCCAATAAACTCAACATGATTCACTACCTTTTGCCCAAAACGAAGGCGTTTGTCAAACCACATTTGTTACAACGCTTACAGTTTGTGTTAACTGATTCGGAGTTGTTGGAATTAAAGCGCTTTTCCTTCTTTCAAGCACTCAACCAGATCCCGGGCTTTCAGGGTGAGCAAGTTGAGTACTTTAACAGTAAGCTCAAACAAAAGTTTACCTTGACCTTAGGGGAGTTTGAAATTGCCCAACAGCCAGATGCCAAAGCATACTTTGAGCAGTTAATTACCCAAATCCAACAGCTCTTTTTAAAAGAGCCGGTTAACGCTGAGTTTGCCAATGAGATTATTGATGCCTTTTTGGTGAGCTATTTCCCCCTCCATCCCCCAGTACCTTTGGCACAATTAGCTGCCAAGATTTATGAGTATGTCAGCCAAATTGTGCTTAACGAAGCGGTTAACCTAAAGGATGAACTGATCAAACTAATCGTTCATACGCTTTACGAACAGTTAGATCGTCCTGTTGGGGATGAAAATTAAAATATAGCTCAGATCTAGTTTAGAATGAAACAATACAAGCTTGTCAATACAACCCAAAAAGAAAAGACGCTTTGTCTTGAAATAGCAATTGACACGAAGCTGTGAAAGGAGACACAGCAAAAGCAAACCCAAGATTTAACCAAAAACATGAAAATCAAGGGTTTTCGTAAAGGTAAAGTGCCTCCAACTTTAGCTAAGGATTACTTAGACCGTGCCGAGTTGTTGCAACGTAGCGCCCAAGCGGTAATTGATGCCATCTTCCAGCCGTTACAACAAGAAGCAGTAATTGCTGACAACGAGAATGTAATTGAGGACTTTCCTACCATTGACTTTAAAACAATCAATGAGAACGATTGTGTTATTCTGTTTGACTTTGATCTCGTACCCCAGTTTGAACTACCTGATTACAAGCACATCAAGGATTTAAGCCCGATTGTACCCCTAAAGGATGAGGAGTTCAACAAGGAGTTGCACAACATCGAAAAGAACAAAGGGAAACTAGTTGATGTTAGTGACAAAGCATTGGCCAACAATGATATTGCCGTCATTGACTTTGTTGGCAAGGTAGATGGTAAGGTGCTTGAAAGCGCTACTGCCAAACAATACGAATTAACGATTGGCTCCAACAGCTTTATTGATGGCTTTGAAAGTGGTTTAATTGGCATGAAAGTAGGGGACAAGCGTCAGTTAAAGTTAAAGTTCCCCAAGGACTACCATGCTGAAGAATTGAAGGGTAAACCAGTAGAGTTTGACATAGAGCTCAAAGCAATTAAGCAGTTAGAGATCACCCCCATGGATGAAACTAACTTTAAGGAGTATTTACCCGCACAATACCAGGGTTTCAACTCACTCAAGGAATTTAAAACTTACTTTCATAAGTTAGTTTCCGCTAAGAAACTGGAAATTACCCTACAAGAAAACAGTGTTAAAATTCGCCAGTTCTTTTTAGCTAACACCACCTTACCTTACATTCCTGATTCCTTAATTAAATTGGAATCCGATCGCTTACTGAGAGCGCAAAAGGATCAAGCGGAGCAGTACAAGATTCCCTTTGAGAGATTGTTAGCAGCTTCCAAGCTTTCATTGGAGCAGTTGCAACAACGTAACATAAAAGAAGCACGTGATAACGTTACCTTTGCTTTAGTGATGAAACGAATCGCTGATGTGGAAAAAATCAAGGTTGATAACAAAAAGATTCACAGCGAAATCGAATCGATTATTGATGTTGAATACCCCTTTGTTAATGCCGAGTTGAAAAAACAAATGTTCCACAACATGGAACAACAAAAGGACTTTGTGGAATCGATAATTTTGAACCGTTTAACCACTACCAAGATAATTGAGTACTCCACTCATTAGCACTCAAAGTATTAGAGTGCTAAAAAATGTGTTACAATTTAGCATTCTTCAACCTCTTATCTTAATAACTATGCCAGCTGTAAAAAAACCACAAATTCTCGTTGTGCGTAACCAAGTTATCTTTCCGTACAACGGCTTTGAACTTGATGTCGGTCGTGAACGCTCGAAAAAACTAATTAAAGCACTCAAAAACCTCAAAACCAAACGTTTGGTGTTAGTCACCCAAAAGAATTCAGATCAGCTCAACCCTGAGTTTGATGACATTTACCATTGCGGTACTTTATGTGACATTGATGAAATTATTGAAGTACCGAGTGAAGATGGCAAGACAGCTGACTATAAGATTAAGGGTAAAGGATTACAGCGCGTTGCCATTACAAGCTTCTCTGATGCTGATTTAACCAAGTACGACCATCACTTTCTCAACTCTACTCTAACAGAAAATAAAGCGTTAGATAAGTTGTTGGAACGGATCTTTCCCGATAAAGAAGACTTTGCTGAAATTTTAGACAGCTTAAACAGCTTCTTGGAACTGCAAGAGTTAAAGAAGCTATCTAAGGTACCAAAAGACATAAAACGGTATGACATTATTACCTTCAAGCTTGCTAGCTTAATTTTCAAAGACATTACCCTCCAACAAGCCATTTTGGAAGAAAATGACATTGAAAAACGGTTACAGAAGATCATTGGTTCTGGCATCGAGGATTTAGGTCACATCAGCGAAGAAGCCCGCGCTAAACAACGCGAAAGTGAGTTTGACAAGATTGACAACCGCATCACGCGCAAAGTTAACGAACAGTTATCGCGCCAACAACGTGACTTTTACCTCCGGGAAAAACTCCGGGTTATCCGTGAAGAGATCGGGATGACTTCCAAGAAGGAAGATGAAGTATCCAACATTCGCAAAAAGCTAGAGGAAAACCCATATCCAGAGCACATTAAAAAACGGATTTTAAGTGAGTTAGATCACTTTGAAAACTCCTCCTCTTCATCACAAGAATCAACCTTAACCAAGACTTACATCGACACTTTGATGAACTTGCCATGGTGACAGGAAAGCAAGGACAATGCTGATGTAAAAAACCTGATTAAGATCTTGAACAAGAACCACTCAGGACTAGATAAGGTAAAGGAACGAGTGGTTGAGTATTTAGCAGTACAGCTCCGTACCAAAAAGCTTAAGGGTCCGATTATGTGTTTAGTCGGTCCACCTGGAGTTGGTAAATCCAGTTTAGCCAAATCAATTGCTGAAGCGTTAAACAAGCGCTTTGTTAAGGTTTCATTGGGCGGGGTGCATGATGAATCGGAAATCCGTGGTCACCGTAAAACCTATTTGGGTTCGATGCCAGGACGGATTTTAAAGGGCATGGCACGTGCTAAGGTTATTAACCCACTGTTCTTGTTAGATGAAATTGACAAGATGACATCCTCCAATCAAGGTTACCCTTCCGGCGCTTTGTTAGAGGTATTAGATCCGGAGTTAAACAATAAGTTTAGCGATAACTATGTTGAGGAGGATTATGACCTGTCTAAGGTAATGTTTGTAGCCACCGCTAACTATATCGAAGATATTCCGGAAGCCTTGTTAGACCGGATGGAAGTGATTGAACTTACCTCTTACACCGAACAGGAAAAGCTACAAATTACAAAGAGCCACTTAGTTAAGCGTTGTTTAGATGACGCTGAGATTAAAACAGATGACCTCAAGTTTACCGATGAGGGTATTAGCTATATCATTAAGTTTTACACGAGGGAAGCGGGGGTGAGACAGTTAGAACGCTTAATCCAGCAAATTGTGCGTAAGTACATTGTTAACTTGCAAAAAACCGGCGAACAACAGGTAGTAGTTGATGTAGATTTGGTAAAGAAATACCTCAAGAAAGAAATCTTTGACTATACCGTGCGCGATGAAGATGCACTTCCGGGAATTGTTAACGGCATGGCTTACACCCCAACTGGTGGTGATCTGTTACCAATTGAAGTAACCCATGTAGCTGGTAAGGGTGATTTAATTCTGACCGGCAATTTAAAGCAAACAATGCGTGAAAGTGCTAGCGTAGCGTTAGGTTATGTCAAGGCCAATGCGCAGAGCTTTAACATTAACCCTAACCTCTTTAAAAAGGTTGACATTAACATCCATGTTCCAGGTGGTGGTATTCCCAAGGATGGCCCGAGTGCTGGCGCGGCTTTAGTTACTGCCATTATCTCGTCTTTAACTGGCAAAAAGGTTGACCCTAAGATTGCCATGACCGGGGAGATTACCCTAAGGGGTAAAGTAATGACCATAGGCGGGGTGAAAGAAAAAACCATTTCCGCCTACCGCGGTGGAGTACGCACCATCTTTATGCCCGAAAAGAATGAGCGTTACTTAGATGAAGTGCCTAAAGACATCGTTAAGGACTTGGAGATTATCCTAGTAAAGGAATACAAGGACATCTACAACAAGATATTTAATTAATTTTTACCAATTCAGGACTTCAAGGTGCCTAAGATCAGATAATTACCCTTTTTAAGGGTAAAATGGTCATCATTAAACTAATTCATTTCTTATAAGTGTTAGCTAGAAGAGGACGACGGCAAAAGAGTGCTGTAGGACTTACTTATTTTGCTTACCTTGCACAGCAAACACTCCGCAGCAAAACCTGCTGGGTGATGTTTGTGCTGTTGATGCTGGCGTTAGTGATGCTGTTCATTGTTCAATCGAGCACCAACGTGATTGCGCTTTGAACAAACAATGTCTTTCAGTTTTCACTCATCTTAATACCGTTAGTGACACTGTTAGCGGGCAGTATTACCGCTGACATCTTTATTGAAGGGTACAACAACGGGATTGAGCTGTTCTTGTTCTCTAAACCTATTAGCCGTGACCGCATTTTGCTCATTAAGTTACTAGTGCTGTTAATGTACTTGAGTATTGTAGCCGTACTATCCACTGCTGTAGTGAGCTTGGGTTACTTTACGCGCAATGAGATTGAGAGTTATTTAGATGATTTAGCTTTAAGTATCTTTGTCGGTACCATTTTAAACGGCATTATCTTTAGCGCTGTAACTATTATGTTAGCCACGCGCTTTAGCAACATCCAGAGCTTAATCATTTTGCTCTTGTTTGTGTCGTTGTTTAGCTTTTCCTCACCAATTGCGCGGTTAATCTTCGACACGCCTGCTAAAATGTTGAGCCAACAAGGTTACAGCGTTCGCCAAATTAACGGAATTGCGCACAGCGAAAGCCCTAATGTAGAGCGTAAAATTTACGTTTCGGTCAAAAAGCGGCGTTTAACTGGGGTGGATCGCGAAGAAAAAGAGCTTTGAAAGAGTGATGAGCAAGCAGCACTGCGTGGTAGAAATTTGTATGAGGAGGCACAACGAACCTCCTCTTACATTAATTCCTTTTACACCAACATTGGTTACTTATTTGGCTCGCTTTACCGCTTGGGTGCGTTAGCTGATGAAAATAACTTTGATGCATCAGTAGCAACCCAAAACACCAAAATTCGCTTTGGTAAGTTAGTTGATTTAGATAAGAGTACTGACATTATTGGTTATCGCACTAAAGACCGCTTTGGCCGTTTCTTTCGCTTTCACTTGCGCTTTCCAACGCTCAACTTTAAGCAAAGCTTGAGTAACAGCGGCGTTTTCTTTATCAATAAACGGGGATTAGTTAAAGCTACTTTTAGTGCTGGCGATGATGCCTTTAAACGTGACCCGAAGATAATGCAAAAGGTGTTTGACACTTTTCTAGAAAGTTTTAGTTGATTTTTAGACAAGAAAATTAACCGCCGGATTGCCAGAATTTACGCTGAAAGTGATAACAAGGGTAGTCCTTTCCCAAAGGAAGGAGATGATGAAAATGCTAAGGGGGATGACAACAGTAGTGAAAAAACGGATACAGTCTCTGTATCAACTAAGTTAAAAACAACTGCTGATCAATCTGAAAGTACTCAGATGTCTAGTGAAAGCACTGCCACCGGTATTTCTAGTGATCCCCAAAGTCAGGGCAAAATGAACAACAAAAGTGAGGAACAAAAAAAGAAAGAAAAAGAACTCACTTGACAAATCTACCGTGATATTAAGTTTCTCAATTCCATTTATTTCTTACAGGTAAATAACGAACGTTTGTGGGACAATGTAGCTTTTTTAAATAAAGTGAACGCTGAAGACCAAAACGGGGATAAAAACCAGTTTGCCCAAGCAATGGCATCACTCAATAAGCAAATTAACATGTTTTACGTGTTGAATGAAATTACCAACAAGGAACACAATTTGCCAACTAAATACGGTGCTTATTCTGATCAATTAAAAAAGGTCATAAACGAAAACTTTGAAAAGATAGAAAATAAGAAAAAGGAGATAGAGGAAAAACAAAATAAAGAATTTCAGGAAAACAAGAATGGTGCATCAAATAATCAAGACACAAAGCAAGACGCACAAAAAGGTGATACCAATACCCAAAGCACTGAGTTAAAAGCTCGTCAAGCACAACAAGTGCAAAAGGATCAACAAAATGACAGTAAAGGCAATACTGCTACAAACAGTGATACTGGTAAATCGGACAATAAAACCGATACCACTGAAGATGAAAAAAACACATATAAACCCTTAACAGCTAGGGAGAAAAAACCAAAATTAAGGAGCTTACTAAGGACATCTTCTTCTCTACAGCGCAGAACTATTTGTTCTTAGTGCAAGGTGGGTATAACAACAAGTTGTTTGCTGGTGGTTTACCAGAGCTCCACCAACGCTTCTTGTCAGACGTAGTGGTTGATTTCTCTGAGGAAAAACTCGTTTATGAAGTACAAGGTGAACCGATTGTATCCAGTGTAGCCACCATTGTGATTACCCTATTTTTAACAGTTGTTTTATTAGCTATTGCCTTTTTCTTCTTTAAATACCGCAACGTTAAATAGTTATGTCTGTTACACCAATTGTTGAAGTTAAACATTTAGAAAAGGAATTTGGTTTTTGACGCAAAAACCGTATCTTAAAGGATGTGAATTTTGCCATTATGCCCGGTGAGTTCCATGCCTTTATTGGTCAAAATGGTGCGGGGAAAACTACCACCATTAAGTGTTTAATTTCATCTTATCAGCGCTTTAAGGACGAAATTAACATCGATGGCATTAGCAATAAAAACGCCAAGTCTAAGGGTGTAATTAGTTATATCCCTGAATATGCTGTGTTTCCCAAACACCTCAATACCCATGAATACCTCTACACGTTGGGTAAACTGTCAGGTTGTAGTTTGCAAACCATTAAGGAAAAGGTTGATTACTGACTAGCACGTTTTCAAATTGAACACCTCCGCTTTAAAAAGCCAAATGACTTTTCTTCTGGACAAAAGAAAAAGGTTTTATTAATCCAGGCCTTATTTAATGATCCCAAGCTGTTAATCATGGATGAGCCCACTGCTAACTTAGATCCCAAAACACGTAATGAGTTTATGGATGTGTGTTACGAACTTAATGTACGTAACAAGATGGCGGTGTTTGTTTCATCCCATATTTTGGCTGAATTGGAAAACTATTGTGATTCGCTCACTGTTATTCATGAAGGGCAAATTCTTTTTAATGGCAAAACAAAGAACATTGCCAAAGACAACTTTGGTTACCGTTTAAAGGTTAACCATTCCGATAGTTTACGTAAGTGATTAAAAGCACAAAAAATTGCTTACAAGTATTTCCCTGCAACCGATGACTTTCAAGTTGACTTAAAACAAGAGCAATCCAACAAATTTGCAGTTGAGCTTTACCAACAACCTGACTTTGAGGTATTTATTTTTGCACGACAAGGCAACTCGTTGCAAAACATTTACAATAACCTAATTGAGCAATACGAGTACGATCAAGCCCAGCGTGCTGTTGCTATCCGTGAACAAGATGAAGCTGTTTAAAAATTGAAGCGATGTTAACAACGTTAAACAACGGCAACTTTTACTAGCTTACTTCCGTTTTATCTTAAAACAGACCGTTAAATCTTGACTTTTATGAGTTACTGCTGGACTAGTACTGTTTTTACTGGCTTTAGTGCTGTTAATCATCCCCGCTTTCACCAAACAAGATCCTTTGTTTTTGTGATCACACCCAGTGGTGCAAATGTCGAGTTTAATCATTCCTTTCATTGCTCTGTTTGCCACCATTATTACCTTTCAAGTCTTTATTAATGGTTATTACAACGGTCTAGAGATCTTATTAATTACCCGCTTTTTTACTCGCGGTCGGTTATTCTTTGCTCGCTTAGCAGTCTTGTTTATTTGGATTACTGGCACGGCATTTTTAAGTGGTCTTTTTGTTAGTTTAACTGCTACTTTAGGTGCTACATCACAAACAGTTACTGACCTTGTTTTAAGCGTCTTTTTTGGAGTGCTTTTACTTAGTTTATTGTTTAGCTGTGTCTTGATTATTATCGCGCAGTTTTTAAACCGCACCCAAAGTATGTTGTTGTTACTGTTAGGGGTTTCCCTAGCTAGCTTTACAACCGTTATCTTGGCCTTTTCAATTAAGCCACCGAGTGAAAATCTCCGTGATAATAGTTACCAACCACTAAACCTAAGTCTCATCAGTAAGTCTAAGTCCAAGGGTGTCGAAAATGTCTTTACCATCATTGACTCTTCAAATGTATCCGATCCGCGCAATAAAAAGAAATCGATTGTAAAAACCAATCCGCAAGCGATTTGGGATGAATATGGGGAGAGTAGCTTCTTTCAAAGCCAGTATTACTGGAATGTAGGGTATTGAATTAACAGTCTGTTTCGGTTAAACAGCCTCAGTGATTACCGCAACTTTGATGTTAACCTCTATTTTTTAAACACTAAGCTTAATTTTGACAGGGAAGTAGATACTGATAAAATGGTCGACTTTGTTAGCTTTCGCGATCAAAGACACCTTTACTTTTTAAGTTATTCCTTTTTTATCAATACCCATAACCTGCCCACACAACCGTTACCCCGGATTCTTACTTATGACAATAACGGTGCCTTTTTAGAGAAAATAGTCCCTTCCTTTGATGACATAAAACTAGATCCACCCCGGGTCAAAAAGGTTTACAAATTCTTTAGTGACACCATTATCCAGTCCTTCCGTGATTATGAAGCGGAGGAAAAAGAAAGGCAGGAAAAGGAAGAGAAAGAAAAGGCTGAAAAGGACAATGGCAACGGCCAGGATTCCAATAAAGTTAACTCTGTCAGCACGGAGCCTAACAATAAGAATTCATCAGATGCGGATTCTAAGGATAACAATGATTCATCCGATTCCCAAGGCAAAGACTCAAGTAAGAGTAAGCCTAAGTTTAGACCTAGGCTGCCACAATTCTTTGATCGTGTTTCCATTAACTACAGTAAGTTTTCCAGTAGCTTTAACACACAACTAAAACACATTCATCAAGGACTTTCTACCCGTGATGAGGCCATGGAAATCTTTAAAGATAAGGTAGCGTTATTTTATGCCCTATCAATGGCTTATGACAACTTTACTTTCCAAAAGGACAGTGGCATTATTGATAACACTAGCTTAAACAAATTTAAAACAGAGTTTAACAAGAAGCAAAAAGAGCTAACTGAAAAAAACAAGCAAAAACAGGACGGTAAAGACCAAAAGAGTCAAAGAATGACACAAGGTGCTGATAAAGCAGTCACTGTTGAGCCAAAAGCTATGCAGTCTGAAATTGGAATGACTGATCAAACTAATGACCAAAGCAGTTCGGAAACAAAGGACAGCATGGATAGTTCAGACAGTAGTGATACTGTTGATAACACTGATGAATCAGAAGATAAACAAAGTGAAGAGGAGGAAAAGTTCGACGAGGAAATTGAGAATGCCAAAAAAATGCCTAAGGCTGAAGACGCCTTTTTTAATACTGCCTCTATCTGGTTGAGCTCACCATTTCTCTTTTTTGAAAATGGTGCAAAACGCGAACAACGATACGAAATCCATTTGCTCAATTCAAACACACAGGTTAATAACGAAATAATTAAAACTAACAGCTTTTATTACGTTAGTGGTGAACCGATTGTAGGCCAAGAAGTCATTATTGCAATGGTGCTGGTGGTAACACTAGGTCTGTTAGTTGGTAGTTTCTTCGCTTACCAAAAACGCGATATTAAGTAATTAGTTTTTCTGTAACGACCTAACGTACTTCAAGGTAATTTGGTAAGCTAGTTTTAAATCCTTTTTCGCTAGTTTAACCAAAGCGGTAATATCAATGCCTTTAGCTCACTCTTCATGAACGCGCATTGGTTCTAACTTATCCGCTAAGTAGATAATCATGTCTAGTTGACTCATCTTTTGTGGTGGTACAGTGTGGTTTCAAATAGCGCTAAAAACAGCACTATTATTTAAACCATACCAATGCTTTAAAATGTATGCACCTACATAGCTGTGTAACACCTTTCAGCTAGGGTAATCATTTACACCCGCTACTTTGGCTAATTTCTCTAATTGTGCTTGGGGTAGTTGCTTTGCTAGATCATGGTAAGCACCAGCAGTATAAGCCGTTTTACCATCGAGTTTATTTGCCACTGCTAAAGTTTTAGCTAACTGACCAACGCGCAAACAGTGTTGCATGCGCGGTTCATCTAACAGTGTTTGCAAAATGGTTTTTAGATAAATGTTGTGTTGGTGGATATAGTGCAACACCTTAGCGGGAATTTGTCGAAATTTATGACTACCAAACATTTCACTCGATGCCAAATCTAAAGGACACTTACCCAACAGTCGGACGTTAAACTGTTGTACCAATTGTTCATCAATTGGATAACCGAAACGTTCATAACAGACAAAACGACAAAGTTTTTGTAACTGCTCAATGTTATGTCATTTGGCCAACTCGCTTAATTTATCACTGCCAATTAAAAAGTACAACTCAGCATTGGGAAAGCGCTGCTTAAAGTGTTGTACTGTATTGAACGAAAAACCGTTTTGCGCTTTAATGTCAAAGTTAGATACTGCCATCTGAGGCAATGCTTTTAGCGCAACCCTTAACATTGCTAAACGTGCTTGATTAGATGCTTTAATCGGTGATTTAAAAAAGGCTTTAGCAGTTGGTACAAAGTAAAGCTTTTGTGCTTTAATGGCTTGTACAGCACGCTTAGCAATGTAGATATGCGCTTGGTGGAGGGGATCAAATGCACCACCAAAGATCACTATTTTTTTTCTCAAAGAGCTTAAATTGTTTAATTCTAACTAAAATAATCCTGATAGCTACAGCCATTTGTGCTGTCCAGGTCCATGGTAAAAAAGGAACTAGAAATTTACAACCTCTATACTTTTCAAATAGACCTCGATAAAAAGCTTTTATTTGAAAAAGCAGATAATCAACAGAATTACTCTAAGATTAGAGCTCGTTATTTTAAAAATTCTGCACGTAATCAACAAGCAGTTTTTCTCAATAAAAACCTGATTAAAAACACTTTTAATAAGGCACTGTTAAACTTCTCGGATTTTTTGAGTGGTAGTGGTGTAGAGAGCATTTTTAAGCAGGTCATTGATGATCAACCTGAAGTTTTAAACTACCTCAAACAGGTCAAAAAGGAAGATAGTTGCGATGGTCACAGCGAAGCGTCACAGTTAGTATTTAATGTAGTCATTAACCCTAAAAATACGCTCGCTAACTTCTTTGAGGAATTAACCATTTACCTCCACTTTAATGAAGAGAACAGCACAGTTGTCGGAAGCTTTTCGTTAAAGTGAAACATTAAGCGTGCCGACCTGTTTTCAGAAACGAAAAATATTGCCATTAACAATCTGATTCACACCTTCTGTAAAAACAACTTAAACGAGGTTAGCTTTATCCAGATCATTAAATGCTTTGCTAAAACACTGATTAATAAGCAAGGACAAATTGTGTTGGAGAGTTGTGCCTTTAAGCAAAAATGACAAAACATCGTGGAGCAAAAGTACCCTTTTTCCACGATTCACAAAAACTTAAAGATTGTCAATTCTGATTTCTTTGATGCTTTTTTTGTGATCTTGTTGTTAATTTGTCACCTCAATAATAACTTGTTGTGGTTGTGTGAAAAAACTGAGCACTTTGAATGAAAGCTCAACAGCAAGATTAGTAATTTCAAAGAAGATAACACTGAAGTGTACTTGTCGAAAATGCTGTTGTTTTTAAAGGACTGGTACTTTGAAAACCAAGCCGTTACTAATGAAGACATTGAAAAGGTTGATGAAGTGGAAGACATTGGTAAGTTAGTGGAAAAATACAGCGCTAATCAACCACAAAAGTTGTCTTCTAATTCAACCGTTTATGTATTTGAACCAGACAAAAAACAGTGCTTTTTAAAGAATGATGACTTTTTCAACACTAACGAAGCTAAGCTGTTGTTTTTAATCACAATGCAACCCAATGTTTTTGGTCTGGATGATACTGCCATTGCCAATGACTTAAACCTAAGGGAAATTGGTGACTTCTTTAAGGAAATTGACTTTACTGACAGCGATGTGCTCAATGACTTTCAACAACAAAAAGAAACTTTGTTAGTACGGCGTACCTTTAACCAACTGTTGTTTATGAACAGTAATACCGATGTTTTGAGTATTGTTAACAATAAGTTCAAATCAGCGATCCATAACATTGTCTGAACGATTACTTACAGTAAGGCAATTATGCTCAAGGCATTTGATTACTCCAAGATCTTTGAGCAAAACCGCACTAACGATCCTTCTCTCTTGCGTTCTAACCTCAACAGCATTAACCGGCTGCGGTATCTCAGTGAATACTTCCGTACCGCTTCAGTTAAGTATGACCAGCTGTACACCAAGGTAAAGGAGTACATGCAATTAGATCAGTTCTTAGTAGACATGATTAACCAGGTCAACCATGAAGATGAGATCTTTGGTAAATACAAGGAACGGGTTTATTTGAGTTTGGGAATAGTTACTGCTGTTGTCTTTGGTATTATTGAGTTCTTTAACTGTGTTTGAACGGTTTTAACAGTCAGTCAACAAACGGCTGAAAAAAGTTTAGCTGATCCAAGAAACACGGTAATTATTGGTATTGGTACTATCCTTGTTTTAACACTGTTAATTACCATTTTAACCTTTATGACAAGACGGCTTTACCTGTTTGAATTTAACAAGAAGCATAAATAAAGATGGAATTCAATAAGTTACAACTCAGTCACTGTATTTCCTTTTACATTTCAGACGTTTCGGAAGTGTTCTTTGAAAGTATTAACCAACACCCTAGTCGTGATTTTGTCAACAACATCCTGCAAAAGATTAAGAGCACTCTCAGTGAAGAGGAATTGGAAAAGCTCAACACCATTGAGGAAGTTACTAAGGATGAGAAAATTTTGATCATGCTCAACCATGTGTTGAAGAAGATTGTTTCTAAAACGGGTAGTTCTAGCTGTGACTTATTTCAAATAGTGAAACACGACCGCTTTAACGAGCCGGTTTACATCCAAAGTGTCAATGCCTTTGAAAACAATTTGATTAACAATGAATTTGCGGAAAGGCGGTATGACTATTTAATTGAAATTAATAAACACTCTTATTTAAGGAAGTATGTCAATGCCATTCGCATCTCCTTCTTTTTAGATTTAAGGGCGCAAATCTTGTCCGGTTCCTTTACTTTGGATGTGATTAACAAACAGTTGGAACACCAAAATAAGGAAGAGTTGTTCCAAGCGATTTACTTACGCTCCTTAATTAAGCATTTTATCTCGAACCAACCTTACCCGATTTCCTTAAATTCGTTTATTTTTGGTGATAAAAACCGTGAAAACAAGACGGTTGAAAACGACAAGCTTAGTGTGCTTAAAAACAATTGGAATCAAATCTTCTTTTCTAAGTACTTTGATTTTGTTGCCAAAAACAAAGAAGAGCGTGTTGTCGATAACAACTGCGATGAGCTGTTTTACGCCACCATGAACACGCTCTTGATTATGTTGATCATTATTGAAGAGCTCCGGGTATACTTCAACAGTAAAGAGCCAGCCTTAATCTTAAAGCTACTAGATAACAAGGTTTCACTGCGCGAAGACCCCGACCAAAATCCAGAAACTGATTTGCATGAGTTAATTAAGTTTGCTGAAAAGAATTACCTCGAAAAGGAAAAAACAAGTCGCTGACACAAAAAACGCGTCAAAAGCTTAGAAGAGTTACTAGAAGAAATTAAACAAATTAATTTAGAGACTAAAAACGAATCGTTAGCATACCCCGATGAAATTGTGGAATTAGAGTTAGATAACGTTCACAACTTTGTCTCTACTAAACAGGTTTTTCGTCACCAGTTAGACTTACAAACTTTACATGGTATAGTCATCAACCCTGAGCGCTATGGTATTGGCATGTGGAGTAACCACTTTGTCGATTGGGAGGAGTTTAAAGATTTAATTGAACAGATTACTGATGCTGAAAATGGTAGTGATCTCTATGGTTTTGAAAAGGACTTGGATGAGAGCATTTGCCAAGTTAACAAGAAGTACTTAACCTTTATTAGTAGTGACAGTAGCTCTTTCTTAATTATTAAGAATGACCAAACTAAGGTTATCTCTAATTATGTCTGGGCGCAACTGTACTTTGAAACGCGCCGGTGGATTATTAATGATATTGAATACGATCTGTATGAAAAGGGGTTTGACAAGAGCCACTTTGCTAGTAACATTGCCCTTTTAGAGAGCTTGAATTTTAACTGACTCGATCCCTTTTACGGATTGACCTCAATCAAGGAGATCATGCAAAAGATTGACAGTAAGAGTAATTTAAAAACATCGATTGCCGAAATGGTCGCTAAGTTTAAACACGAACAGCGCATCTCCAAAAAGGACAATGAGCGGGTGTTGATGATCTTTGCTTATGTAGCCGCTGCGGTAGTTGGTTTTATTAACTTTTTTTCGATGGTGTTTACCATTCTTACAGTCAGTGATCTCAATGCTGGTCTCACCCCCGCTAACATTGTTGTTATTGCAATTGCGAGCTTATTGGCCTTATTTTTAATTGTTATTGCTGTTTTATTTCGGTTTAGGTGAAAGTACATTAAGCACTAATGCAGGTTAAAGTAATTGATGAAAACAGCAATACAACCGCTGTCCTTAACTGCGCTAAAGCGAAAACGCGAGCGCTAGCTTGGTTGTGTGATACAGTACTGTTAGCGATCTTACTCGCAATTATTTACGGCATTAGTAGCATTTTCATCAAAGAACAGTCCAGTGTGTTTTTAATTATGACGGTAAGTCAAGCAGTCTTGTGGTTGACTTACTTTGTCATTTTACCGGGTTTATGGAAGGGCAAAACGCTCTTTCGTGCTTTACTGGGTTTAAGTTTACTCATCTTTAAAAAACGCTTTTGAAACCTCTTAGTGCACGAGCTTTTTCTGTGAGTATGGTACTCAGTTATCTTTCTAGCACTAGCAATTTACTTTTTTGTCAACAGGGATGATCCGAAGATACTGCAAGCTTTTTTTGATAACCAAAACAGCAATCTTAGCTGGATATTTGTCAAAATCTTGCTAAGTGTTATTAGTGTCTTACAACTAGTATTTGTGGTGTATTTCTGTTTCAGCTCCCAAAAGCAAGCGTTGCAAGATTTGTTATCTAAGAGTTTTATGGTGCAAAAAACAATTAAAGTAAAGGATTGTAAAAGTGAATTGAAGTCCACTAACACTATAAAAACGCACTCTGATTTACCCGGCGACATTGATTTAGAACAATTAGGTGATTAAGTTATGGAACATTTAAATCAGGAACAGAAAGCTGCTGTTACTTGTGATAACGGTGTTAACGTGGTGTATTCCGGTGCTGGCACGGGTAAAACAACCGTTATTGCTGAACGCTTTGCTTACTTAGTCAATGAAAAAGGCGTTAATCCTCAAAGTATTTTGGCCTTTACCTTCACTGATAAGGCAGCGAGTGAAATGCGCCAACGCATTATTAAGTTAATTCCACAAAAAAGCTTGCAGGATCTGCACATCTATACCTTTCACAGTTTTGCCAACAGGTTTTTGCAAAAACATGGCAAAAGTGACTTTGCCATCTTGAGAGATTCTAACCGCTTCTTTAGTGATTATGAAATGGGTGATCAACTACAAACAGTGGTGGAAATTTATAAAAACAAAGTAGTTGATTTAGAACTTGACAACCTAGAGTATAACTCCGCTTTTAGAGATGCTTGTACAGATACTTTTAACGAGGATTTTAGTACCATCTCCAATGGTCAGTTTCGTAAACGGGCTGCGACTGCTTTACGGGCTTACCAGAACTATTTAATTACCAATAACCTATTTGATTTTAGCGATTTAATTATTGAAACTTGCCACCTTTTAAAAGGTAATAGTGAATTGTTGCAAGCATTTACCGAAAGTGTGCATTATATCTTAGTAGATGAATTTCAAGACACTAACCTAGCCCAGTACGAATTGGTAAAGTTACTAGCTACAACTCATCCTAATCTCTTTTTGGTGGGTGATAGCAACCAGATGATTTACGGTTGGCGGGGTGCTGTTGTAGAGATCTTTGAACTTTTAAAAAATGATTTTCAAACAGTCAAAGAGTTTTATACCACCCAAAACTACCGCAGCAGCCAAGCGGTGTTAGCAGTAGCGAATGATGTACTAACCGCAATTGCTAGAAAAGAGAGGAAAGCGCTCGTGTTGTTGCACTCTAGCATTGATTCTAAAGCTGTACCGGTACACTATAAGGCCAATTCTTTGAAAAACCAAGACCAGTGGATTATTTACCAAATGAAACAGTTGCATTTAAACAATGGTGTACCTTATGATCAGATGGCGGTGTTATTTCGTAAGAATAAACACCTTGACGCTTTTAGTCAAACAGTGCTGGAAGATGGTGATTTACCTTTAGCTAAATTAAACCTTTTAACAATTCACGCTGCCAAGGGTTTGGAATTTGAGGCAGTATTTGTTTATGGCTTGGTTGAACGCGCTTTTCCTAGTTTGCACTGGGATGGAAGTGATAAGCACAAGTTACTAGAAGAAATGAAACTGTTTTACGTAGCGATTACTCGTGCTAAACAGTTTTTATTTTTAGTTTCTGTTAGTGTGGAAAGTTTCAATGCGTATTATGAGCCGTCACGCTTTTTAAAACTAATTGAAAAGGAACATTTGCAAACACAAAAGGCAGCGTACTTTAAAGAACAATTAAAGACACAGCCAAAACCAGTTAATTTGTATACAGAGACAGAAAACGCGGAAAACTTACAAAAAGCCACTGACTCTAAAAAGTGAATTATTTTAGGGGCGATTCTTTTGATAATTGTGATTATTACCGCTGTGTTAAAACTTTTTGTTGAAAATTAACATTTTATCCCTTTTTTATAACAGATTACACTAATTAAAAAACAATCTTAAACCTAGTACTATGGCATTTAACATTAGCACTCAACTCAATAAGGAACAACGAGCCGCTGTTACCTGTGGTAAGGGTGTCAACATTGTCTATTCTGGTGCTGGTACTGGTAAGACCACCATTATTTCCCAACGCTTTGCTTATTTATTTAACCAAAAGCGAATTAATCCTAGCAACATTTTGGCGTTAACCTATACAAGGAAAGCGGCGAGTGAGATGAAGCAGCGGATTTTGGAGCTGTTACCGGAAAAGTATCATAAGGATGTCAACATTTACACCTTCCACAGCTTTTGTAGCCGTTTTTTACGTGAAGAAGGGCAAAAAAACTTTGTGATTGATGATGACTTAAGTAACTTTCTAAAAGACTTTCTCAAAGAGAGTGAACTAAAAAGTCAAAAGGTATTACAAATTATTGACGGTTTTAAAAACGCTTACTTTGATTTTGATACCAACAGCTTAAAGGATGATGAAAGATTGGTCGAGTTGTGTGAGTTTCATTTAGACCCCCAAGAGCGTAACTTTCAACTTTTCAAAGAAACCGCTATTGCTGCTTTTGTTGAATATGAAAAAGGTAAACAACAGAACAATAAAATTGACTTTGCTGATTTATTAATTAAAACTTGCACTTTACTGAGCAAAGATCACAAATTATTGCGCAAGTGATCGAAAAAATTTCAGTACATTTTAGGTGATGAGTTTCAAGATACCAACCAAATTCAGTATGAACTAATTAAGATGTTGGCCAGCCATCACCAAAACCTTTTTTTAGTGGGTGACAATAACCAGATGATTTACCGCTGAAGGGGCGCGGTTAGTGACATTATTGATTCTTTAAAAAGTGATTTTAAGGTAAGACCCGAAAACGAGTTTTACATTACCCAAAACTACCGTTGTGATCAGAACATCTTAACGGTGGCAAACTCGATTCTAGGCACCATTTATGCCAAAGAAAACCAACCTGATTCAACTAAAGGCTTTCTTTTTTCTGCCATTAAATCAAACCGGCTTCCAGTTTATTTCCAAGCCTCTTCAGTTGAAGGCCAACATTCTTGAATTATCAATAAGATTAAGAATTTACATAAAAACCATGGCATTCAATACAAAGACATGGCTATTTTGTTTCGCACCAACAGAAACATGGATTCGATGACTGAAGCGCTTGAAGCTGATGGCAGTATTCCCCTTAAGCAAAATAAGGGTTTTTTTAAACAGTTAGAGACTTTTAAAAAGGTATTAGTGGCACTCATAACTAGGTCAAACTATGACATTAAACTAGCTTTAAAAAGTTTGCGGGTTTGACCTAATGTTTTAAATAAAAGTCTCACTGTAAACGAGCAAGTGAATTTAGATAAAATCTTGCAAAACTTAGAGCAAGCCATTTTTTTAGATGAGCATACTCGGGGTGAGTTGACGGAAGCTGCTAAAGTTTTTACGCGTTTAATTAAGTTTGTTGAAGAACAACAGTTTGAAGCTTTATTAGCCTTTACTTTTGAGGCATTAAATACTGATCAGTTTGTCTGCAACTTTATTTTTAGAACATTGCAAAAACTGCAAACTGAAAATAAAAACTTCACCATTACCGATTTTATTAACGAGTTAAGGTTCCAACAAGATGAGTTAAAGCAGTCCAAAGATAATGTGATTAATTTAATTACAGTGCACTCTGCTAAGGGCTTGGAATTTGAAGCTGTCTTTATTTATGGGATCAATCAAGACAACTTTCCGCTCAAATCGAAGAATCAAATAATAGATCTACAAAGAGAATTGGATGAACTACGCCTTTTTTATGTGGCCTTGACAAGGGCAAAGAAATACTTGTTTTTACTTTCAGTTTATCAAATGAGTGGTGAAATTATTTACCCCTCTAAATTCATTCGTTTTATCAATAAAGAGGATAGACTAGAAATTGCCACCATTAACCATAAAGTTCACCAGGATGATGAATTCTTCGATTCTTCTAAAACAGAAGACTATCAAAAAAAGTATTTAACAGAAAATACGGATTTTGTTAATGGTGACTCTGTTAGCCACCGTACTTATGGTAAGGGCGTTGTTGTTGAAGTTAGAGAAGATGCAGTCTGTGTCGCTTTTAAAAACAGTAAGTACGGACAGAGGTGAATTGTTAAAAATCACCGTGATTTGGTGAAGGCTGTGTATTAGTTATGATCTGCTATTAAAAAAGAAATTGTAATAATAAAAATGAACAAGTTTTTACCAATTTAATAACTAAAATTAAAATCCTTATTAATTGTTAGTAATAACAACTTTCAACATAACTAAATAACTAAACTCACTATATCATGGAAAAGAAACGTACTGAACAACGTAATGGCGTTGAAAAGAAGATTTGAGAAATAGCTGATAAATTAAGGGGAACCATTGATGGATGGGACTTTAAAAGCTATGTTTTAATTGGTCTTTTTTACCGTTTTCTTTCCGAAAACCTATGCAAATATTTTAACGATTCTGAAAGAAGGAATAATCCTGATTTTAGTTACGAAAATTTAACAGATGATTATGAAGCAATTGATGCATTAAAAGATGCTGCCATTGCAAGTAAAGGCTTTTTTATTAAGCCATCTCAGCTTTTTCAAAATGTTGTTAAAAGTATTCGTGAAAATAAAAATAACGAAGATCTAAATACAACTTTGCGCGATATCTTCGATGATATTGAAAAGTCTACTGAACTAGGTGATGGAAGAAGTAAAGAATCCTTTAAAGGTCTTTTCAAAGACTTTAATGTTTCTGAGGTTAAGTTAGGTTCTACTTTAACGATCCGAACAGAGAAATTAAAAGAACTTTTAACAAGCATTGACACAATGGAGCTCGATGAATTTGAGAAAAATTCTATCGATGCATTTGGTGATGCATATGAGTTTTTAATTTCCATGTATGCACAAAATGCTGGAAAAAGTGGGGGTGAGTTTTTTACACCCCAAGATGTATCTGAACTTCTCGCTAGAATTGCGATTGGCAAAAAGGACACCGTTGATGATGTTTATGACATGGCTTGTGGCAGTGGTTCTTTACTTTTACAAGTAATTAAAGTTTTAGGTAAAGAAAAAACTTCACTGGTTAGCTATTACGGTCAGGAAATAAACCACACAACTTACAACCTTTGTCGGATGAATATGATTCTCCACAATATTGATTACGCTAATTTCAATATTATTAACGCTGATACTTTAACAACGAAGGAGTGGGAGAAGCATTACGTAAATTGCTCAAATGAAAATGGGTTTGAAGTTGTGGTTTCCAATCCACCATATTCCATTAGTTGAGCTGGTGATAAAAAATCCAATTTAGTAAGTGACGTTCGTTTTAAAGATGCAGGGACATTGGCACCTAATTCAAAAGCTGATTTAGCTTTTGTTTTACATGCTCTATATGTATTAGGTCAAGAAGGTACAGCAGCAATTGTTTGTTTCCCTGGAATCTTGTACCGTGAAGGTAAAGAGCAAACCATTAGAAAATACCTAGTTGATCAAAACTTTGTTGATGCAGTGATCCAATTACCAAGTAATCTTTTTAGCACAACATCAATCGCTACTTCAATTCTTGTTCTCAAGAAAAATCGTGATAAAAAAGATCCCATTTTCTTCATTGATGGTTCTAACGAATTTGTTAGAGAAAAGAAGAATAACCGCTTAAGTCCAAAAAATATTGAAAAGATTGTTGATTGCTTTAATAGCAAAAAAGAAGAAGCTAATTTTGCAAAGGCGGTTGAAAGGGACAAAATTAGGGAATCTAACTACGACCTTACAGTAGGTAAATACGTAAACTCAGAGGCTGAAAAGGAAGAATTAGATATTAAGGTTTTGAATCACTCTATTGATGAAATAGTAGATAAACAAAAAGATCTAAGAACTAAAATTAAGGATATTATTCAGGATATCAAAGTAGATTTTGACAACATTGATATTAATAACTAGTTGATAATAAAGCAATTCTTTGTTAATTTACAAGAATTTAAAAACTAATAAATTAATTTCATTATTGTTGTGCAAATTAAAACTTACAAAATCAAGGATATTTGCGAGATCTCAAGAGGCAAAGCAATTACTAAAAAATACATTAAGGATAATCCTGGTCAATATCCGGTTTATTCATCTACTACTGCTAATAACGGTGAAATTGGCAGAATAAAGGATTACGATCTTGATGGCGAATACGTTACCTGAACTACAGATGGTATATATGCAGGTACTGTTTTTTATCGTAACGAAAAGTTTAATGCTTCTCAGCACTGCGGTGTTTTAAAACTTAAAAATAACGAAATAAGTGCTAAGTTTTTAACTTATGCATTAGGAATGGAGGCTCCTAAATTTGTTAATAATGCTTGTCCTATTCCTAACCTCAATTTATCAAGAACTGAGGAAATTGAATTAGATTTCCCACCCTTGCAAATTCAGCAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGAACTACAAATGGTTATGCTGGAGTAGTTTTTTATCGTAATGGCAAGTTTAATGCATCACAAGATTGCGGTGTTTTAAAGGTTAAAAATAAAAAAATCTGCACCAAATTCCTATCCTTTTTATTAAAGATAGAAGCACCAAAATTCGTTCATAATCTAGCTTCAAGACCAAAACTAAGCCAAAAAGTAATGGCTGAAATTGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAATTTCTTATTTAATTGAGTGCAAGAACAAAAGAAGAACTCACTTTCTACTAATCTAAATTAAAGGTTATTTCTTAACCTTTGAGAGTTTCTTTAATTAAAAAAGAAATTTCTTAATTTTGTTGACATTTCTAATTAAGCCGTTCAATTTATTTAGTGTAAAGAGCGCATATGCATTACACATATAAATTGAAAATCGGGCACTTGTTAAATTTAGCTCAATTCTTATACAAAATTGAACGTTTTAGTTATTTTTTAGATTCTCCAAAGAAAGGAGGGATTTTTTATGCCTAAAATTATTTCTAAACGAGAATTCAACATTCTTAGAAAAACAAAAGAAGAAATCTTTTCTCAAATTGTTTACACTAAGAAGAGAAACGGTCTGCACAAGGCTGCCAAAGAATATTACTTCAAGGACAAAGACTTTACAATAATAGAAGATCAACAGGATCGACCTGATAAACCTGAGGAACTGGACACACCGGATATTCCAAAGCCACCAAAACCTCCCAAGGGTCCTGATCAACCCGAGGAACCAGGCCAACCAGGTGGTCCCGATGATCCTAATTCAGGAAATAAAAAGATGCCAAAGCCAGACGAATTTGTAACCCACAGACAACTTCAAGAATTTAAGAAGGATCTTTTAGTGGAACTTCACGAAATCTTTCCTACAAAACCTGAATTAAAACGGGTTGAAGAAAAAGTTGATGTTCTGTTTGAACTTCAAAAAGCTCAGGGTGAACAAATAAGAATCCAAGGTGAACAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGCGAAACCCTACAGCTAATCTTGCAAACACTACAAAAGATGAACGATCGTCTGGATAAGATCGAAGGTAAAATGGACAAAATGGAAAGTAGAATGGATAAAATGGAAACTCGCTTAGACAAACTAGAGTCCAAATAAATCTAATTGAAATAAGAATAACAGCAGTTAATTTTTAGGTTAGCACGTTGCTAATTTATTCATAAAAGAAGAACTATTATTAGTTATTTATTCAGCTTCTTCAGTGGTCTTTAAAGAAATATTAGTATTTAATAAAAAAATTTATTGTTGTTAAAGTTTAAACTCTAGTATTTCTTATTTATCATTAAGTTATTTAATTAACATTAAGTTATTTAATTAACTTATTTAACTTAATCTTCACATGGAAAAACTGGATTTTTTAAAGATAAAAAAAGATTGAACTGTACCAGTTGAATATCAACCTAGTGATCAAAAAATAGAGTGACAATCTTCAAGAAAAATAGTTCAAAATTTAAAAAACAAACTTTTATCCTTGGGTTATGAAGATATTAGCAAGGGTTTTGACGAAATTCCTAAATTCTTAGAGAAAAATTTAAGAAATCAACTTAGCTTATTAAACGATTTTCAATTTTCCGATAGAGAGTGATCAGATTTTTTAAATAACTTTATTTTTCAATTAGGTGACGGCAAAAAAGGAAGAATTGAAAAAATCCAAAAAGGTGAGCTTTATGAACTAAAAGATGAAAATGACAGAATCATTAAAAATGTTCGTTTAGTAAATAAAAGAGAACTATCTAAAAATAAATTGCAATTTATTTGCCAAGATGAAAGGAATGAGGTTATTATATTCATCAATGGCCTTCCTTTTGTTTTAATAAGTATCTTTGAATTTAATTCACCACTTCAATCAAGCTTTCAAGCATGAAAAATTATTAATTCCCTAATAGATGACGAAAAATTTAAAAAGTTTTCCGATTACTTGCACCTTTTTGTTTGAACAAACGCAAAGGAAACAAGATACTGCTCAATAACAACATGGGATAACCTTAAAGAAAAAAGGAAATCAGTAAGAGGATTGTCAAACTCTTCTTTTAAAAGTTTGATGAGATGAAGTGACTCTGAAAACAATAAAATCGACTATATTGAAGACTTTGCAACTCATTTTTTAAACAAAAACGCCTTATTAAACGTTATTTGTAAATTTTGTGTGTTCAGGTCAAATAGTGATCTTTGAGTTATGCGTCCTTACCAAATATGCGCAACTGAAAGAATTTTAGAAAAAATAAAAGAAGACAATAGGAACAGTAAAAACTCTAAAAATGCAAGTAAGGGCGGCTGTATTTGACACTCAACCGGAAGTGGTAAAACACTAACTTCTTTTAAAGCTGTTCAATTGGCTAGTGAAATAGACTTTGTTGATAAAGTCCTTTTCGTTGTTGACAGGAAAGACTTGGACAACCAAACGATCGAAGAATATGAAAAGTTTCAAGCGGGTTCTGTTAGCGAAACTGAGAACACAAATGATCTTAAAGAAAAGATATTAGACGATAGTACCGCCACACGTGCAATTGTTACTACTATTCATAAACTTAAGAGGTTAATAGATCAGAGAAGTAAGCTTAAAGACGAAGATCTTAAAAAGAAAAACATCGTTCTTATATTCGATGAATGTCATCGATCTCAATTTGGAAAAATGAAACAAGAAATCGATGAGTTTTTTTAAAATGGGATTTTATTTGGCTTTACTGGAACGCCTATTTTTGAAAGTGAATATTCAGACAAGAATCAAACTCTAGAGAACCAGCAAAAGACAACTAGAAGTAAATTTGGTGAAATTATTCATACCTATACAATGTCTGATGCTTTGGATGACGAAAATGTACTTTGTTTTAGGTATAAGAGTTATTACATTAAAGATGATGGAAGCCGAATTGATACATTAAATGAAGAGAGAATTAAGCATATTGTTGATTTCACTTTAGATAAATATCTAGATGCAACACAAAATACTCCGAAATTTGATAATTTTGAAAATCCTGTAAAAGAAGGGTTTAATTCGATTTTTGCCTGTGAGAGTAAAGAAGCAGCAATCGAATACTATAAAGAATTCAAAAAACAAATTGAACAAAAGAAGCTACCAATTAAAATTGCAAGCATTATAGCGCAAACGAAGGTAGTGATGAACAAGAAATTAGTGTAAAAGGTCTTGATAAATCCTCAAAGAGTTTTCTAAGTCAAATCATTTCTGATTACAACGAAACCTTTCAAACAAACTTTAGCATTAACAATCTAGATGAATATTACAAAAATGTTTCTGATAGATTAAAGCCAAAAAAGACAAAAAATTTAGATGGTGAAAATAAAAACACAAATGGCTGAGAAATCGACATTTTAATTGTTGTTAACATGTTCTTAACTGGTTTTGATGCACCAACCCTTAATACACTTTGAGTAGATAAGAATTTAAGCGAACACACTTTAATCCAAGCTTTTTCAAGAACTAATAGATGTTTTGGAGAAACTAAAAAATTCGGAAACATTTTTAGCTTTAGAGATATAAGAGAAAACTTCGATCAAGCTCTAAAAATGTACGCAGACAAGCGTGGTCTTAAGGATATAAGATCCAACATGGTTATGCGAGAATTTGAGGAAGTTTTTGAAAAATTCAAAGATTGAACCGAGAAAATAAAACAAAAATACCCAAATCCAGATGAAATTCTTAATTTAGAAAAAGAAGAAAAGTGAGATTTTTCTGATCTTTTTGGTGAATTTCTTGGAGATTACAGAACTGTAAAAAGCTATCATGAATTCGACAAAGACAACCCTTATCTTCCAAGTGATGAATTCAATAAATATTTTGCTGCGTTTTCTCAAATTCAAGAAGAAATAAGGAAAGAAAAGGCAGAGAAAAAAGCGCTAGAAGTACAAGAAGAAGATTTATCAAATGAAGTACGAAAAGAACCGCAAATCTTTTCTTGAGAATATGGTGAGGGTATTCAAGCCAATTTAGATTACATTTTTCATCTAATTAAGGATCTCTATAATTGTGATAATGAGCAAGATAGACAAAAAGTATTAGACAAAATTAAGACTGCAATTAGAACAAATCCAGAGATTCAAGTGAATGAAGATATTCTTAATGAATTTATAGAGGACTTAGGGAAGTCGTTCGAGAAAGAAAAACTTGAAGAAGTTTGAAAAACAGAAGGTAAATTACGTGAGTGTTTAAAGGATTTCTGTAAAAAGAAGGAAGAAAGCAATAAGAAAAAAATTATTAATGACTTTTTAGTTAAAGGAGTTTTCCTTGTTGAATGAGTAGCTGATAAACAACTAAATACAGCTAAAAAGAAAGGTGAATACAGTCCTTCTTGATCTGATACAGAAAAATTAATTTGAAAAGAAAAATCAACCAATCTTCCAACTATGACAGAAAAAGATGAAGATGGCAATTTCAAGTGAAGAAAATTAATTACAGATATCTCGGAAGCTTTTAAAAATCACTTCGAAAGATTTATCCGCTTTGTTGAAATTAGATAGTTTTAAATTATTATCTATAAATCAGGTTTTCAACCATAGATTACTATGGAAAAGAAAAATAAAAAAAATACTGCAACCGTTCCCACCAAAGAACAATTGGATGGCATGTTTAAATCTCTTGAAAAAGAAGAGAACTATGATGTCTTTGTTCAAGTCATAAAAGCTTTAGGTAAGATGTAGTTGTGCGTGAAATAAACGTTTATTATTTAAGCAACGACTCAAAAAGTGATAAAGACGATATCGATTATTTAATAGAAGAACTTTTAGTTGAAGGTTCTCGTTTTAAAAATAGTCTTGTTACGTTTTTAGAAAAAGAAAAAGACTGCCAATCTTGCATTTGTTTATTTAAATCTAAGCTTTCTTCTTCATTCGAAATAAGAGTCAAATTTAATGAATTAAATTTAGATGCAAGTGATGAAAAAACTAAGGAATTTTTAAGATACATAGTTAGTAAATCAATAAACAATGCAAAATTCCTATCTTCTGAAGACTTTGATGACAAAAAAGAAGACGAAATTCTAAGCATCATTGAGAATACCTTTAATAGTTATTGCTACAAAAGCGATTTACTAAGTCCAAGTTTGTATTGTAAAGAAGGCCGCTTGAACATAATTGATTTAATTAACGATATTTTTTGCAAGGTTTTAACTTTTCATAAATTGTCGAACGGAAATAAAAGACTAGCTACAGCCTTATTAGTTAATTTTCTTTACACTTTTGGATATTATTTCAAATGAACAATACCTCTTAAAAAAGAGGAATACTTTAAGTATGCTCACATGATCAAAAACTTTGTAGTTAGTTTTGAAGAACATAAAAGAGAAAACAGGGATGAATTTCTAGCAAAGCAAACAAAGAAATTTATCATGGACAACATCCTTTTAGCTCTCAATTTTTGAGAGACTAAATTAAATTAGTTTTTCTAAAAAACAACAATTAGCTAAGAAAAGTTATATGGACAAAAATGCCTTAAGAAAACAAATTCTGCAAAAAAGAATGGCATTAAGTACCATTGAAAAAAGTCACTTAGATCAAAAGATTAATCAAAAATTAGTTGCTTTTTTAACTCCTAAGCCATGCATTAAAACAATTGCACTTTATGAACCCATTAAAAATGAGGTTACTTTTGTTGACTTCTTCTTTGAGTTTTTAAAGATTAACCAAATAAGAGCTGTTTACCCCAAGGTAATAAGTGATACCGAAATTATCTTTATTGATCAGGAGACAAATACATTCGAACCTAATCAAATAGACTGCTTCCTAATACCATTGGTAGGCTTTAATAAAGACAATTACCGTCTAGGCTTTGGCAAGGGCTATTATGACCGTTATTTAATGCAATTAACTAGACAACAACCTAAAATAGGAATAGCGTACAGTTTCCAAAAAGGTGATTTTTTAGCCGATCCTTGGGATGTACAACTAGACTTAATTATTAATGATGAATAGCATTAAATTCATTTTCCTAGGTGACGTCTATGGCAAGGCCGGACGTAATATTATTAAGAATAATCTAGCCCAACTCAAATCAAAGTATCAAGCTGATTTAGTCATTGTTAATGCCGAAAATACGACTCACGGTAAAGGACTTAGTTTAAAGCACTATGAGTTTCTAAAAGAGGCAGGCGTTAATTACATTACTATGGGTAATCATACCTGGTTTCAGAAGCTAGATTTAGCTGTTGTAATTAATAAAAAGGACTTAGTGCGTCCTTTAAACTTAGACACCAGTTTTGCTTTTCATAACTTAGGGCAAGGTAGTCTTGTATTTGAATTTAATAAAGCAAAGATTAGGATAACTAATTTACTGGGTACTAGTGTGCCCTTACCCTTTAAAACAACTAATCCCTTTAAAGTTTTAAAAGAACTAATTTTAAAGCGCGATTGTGATCTTCATATAGTTGATTTTCACGCTGAAACCACCAGTGAAAAAAACGCCTTTTGCATGGCTTTTGATGGTTATGTCACAACCATTTTCGGCACCCACACCCACGTGCCTAGTGCTGATTTAAGAATCACCCCCAAAGGTAGTGCTTACATTACCGATGTCGGGATGTGTGGTCCGGGATTTGGTAGTGTTATTGGTGCTAACCCCGAACAGTCGATCAGACTCTTTTGTGCTGGTAGCAGAGAACACTTTGAGGTATCTAAATGCGGAGCACAACTCAATGGTGTCTTCTTTGAGGTTGATGTAAATACAAAAAAGGTGATTAAAACAGAGGCGATTAGGATTGTGGAGGACGATCCTAGATACTTAAAACAAGACTACTTTAACCTAATTTAACTGGGTTACCTTATTTTCTGTTTTATTCAATAAACGGTGAATGTTTTTGCGGTGCAATCAAAACACCGCAATTGTTAGTGGTCAATACCACAATACAAAGAAGAGAATAATGTACCAATCATTTTGGTAAGAGATCGCGTTTATTGGATTAGGCTTAAAAAAGTAAAGGTAATCTAACCAAGGCACTAGGATAATGATCGCTAAAATAAAGAAGGTAACTAAGCTAGCTAAAGAAACGTACTTGGTTATTAAAGTAACTAGCAATCACAAAACTAAGCAAATAACAAATCACCACAGCGAAATTGCCAGCAAAGAACCGCCTGTAGTAGCGATGGCTTTACCACCTTTAAACTTAAAGTACAGTGGAAAAATATGACCAATGGTAGCTGCAAAACAACTTAAATAAGTGAGGTAATAAACCTTTTGATACTGGTTTACATTAAGGTATTGTTGTAACCAAAAGCTAAAGATTAAAAAGCTGAAAACAAACGCCAAAAACCCCTTTAAAGCATCACATAAACCAACTAAGAAGCCTATTTTCAAACCAAAAACACGGATTGAGTTAGTAGCCCCCGGATTCTTGGAGCCCAATTTCCTGACATCTTTGTTTAAGATCTTGCCAAAAACATCAGCAAACATCACTGAACCCATTAAATAGCCAATAACAAGGCTAAAGACAATAAGTAGAGCGATGGCGCTAGCTGCGTTCATATGCTTTAATAATTTTATTTAATACATGGATTACTTGACTATCAAACTGGCTTGGATTTAACCGTGCTGTAAAGTTTTTTAGCAAGCCGTTGTAGTATTTCACTAACATTTGTTTATCAGTAAAGTTAACCAAATGCGGACCCAAGATCATTTGTTCTTCAGTTAAATACTGGGTTTGCGCCTTTGTTTTATCCACAGCTAAAACTAAGTAAATAAAACCACGTTCTTCAACTAGTGTTTCAGTGGTAATAGTTCATTGATTAGCACTTAAAAAGCGGCGCAATTCAATGATGTTGGACTGCGGTTGGAGTACAAAGTGCTTTAAAAAGTTATTGTGCTGTGCTAGTATTTGCATAATTTTCAACCCACCTAAACCAGCAATAACACCAACAGCAGGATCTAAGTTTAAGTTAGGTAACAGGTCAAACCCGTCACTGGTAATAAAGTGCATGTTAGTGTGATCTTTTAACAACCCTTTAGCAGCACCTAATGCATTAGCACTAATATCACTGTTAACGCCCACTAAACTAGGGTTTTGTTGAACTAGTTCACGTGCTAAATAGCCATGGCCACAACCAATGTCATAAAAGGCTTTGGGTTTTTGCTGTAAGACTAAATTAGCAATTGTGCTAATCCTTCTTTTCATTTCCCCTCATGAAGGAAAGGCTCATGTGGTTATTACGCACTGCTTGACGCAGTTTGCGAATGGCTTTGGTTTCTATTTGCCGAATCTTTTCGCGGGGAATCTTAATCTTTTGACTTACCTCATCGAGTGTTTTGGTTTCGTTGTAAGGTGGCATACCAATGCGCATCCGCACAATTAATTCTTCCTGTTCGGACAAACAGTTGTTTAACAACTCATCAATCTTTTCGTAATTAGAACGGCTTTCGGTAAACTCATCGGGCATTTGGGCATCAGTATCACGCACAAAGTCACCGAACTGCGATTCTTCATCGTGGCCCACTGTTTTATCTAAAGAAACAGGATCTAAGCTTAAACGTTTAATTTCAGCAATCTTTTTAACTGTAAACCCTTCAGCTTGACCACCCATTTTTTCCGCTAACTCTTCCGCGGTGGGTTCGCGTCCTAATTCTTGGTTTAAGGCACGTTCAGCTTTAGCTAAACGGTTAATAGTTTCCACCATGTGTACGGGAATCCGAACCGTACGTGCTTGATCAGCAATAGCACGAGTAATCGCTTGTTTAATCCACCAAGTAGCATAAGTGGAAAACTTATTACCCAATGACCAGTTAAACTTGGAAATGGCCTTTAAAAGTCCTAGGTTACCTTCCTGGATAAGGTCATTAAAATCCAATCCTCTTTCCAAGTGTTTTTTAGCAATGGAGACCACTAACCTAAGGTTAGAGGTTACCAGTTGATTAATGGCATACTTGCGTGATTCTTCATCAGTACTGTTTAAAACTTTGGCAATTCGCTGCTCAGACTCAAAGTCCAACATCTTGGAAGAATCGAGCGAACCTAGAAAGAAACGTACGTTGTCATCAACCTTATCACGATTAGAGATGTTTTTGGTGGTTAATTCTTCAATGTCCTCGTCAATGATCGACAAGTCTTGGTTAGCACGGAACTCATGGATGTGCTCTTCCACATCATGTGGTAGTTCAATTCCCTTGTCACGCAGTTCATCCAAAACAAAGATAATTTCATCATCTGGGAGTTCAAACTTGGACAAAACATTAACAATGTCCTTGTTGGAAAGCGAGTTATTGTCTTTCTTTTTGTTCTTTTTCTCCCACACCTCCACCAACAGGCTAATTACCTTGTTAGTATCATCATTTTCGGTATGACTCTTTAGAGAGCCTTCTAAAATATCAAACAGCTTAAGGTTGTTCTTTTCCTTTAAAGGTGCGTGTTTTGGCTTTCGACCGCGACGCTTCTTTGGTACATCACTCTCCTTGTTTTCTTGAAGCGTTTTTAAAAAGGCTACGTTTTCGTGTTTTTGTTTACGTTGTGCCTTAGTGTCAACGTAAATCATCTTTTTAAACGGACGGTTTTTACCGAGGATACGACTCTTTTTAAGTTCAGCAATGCGCTCTTCGTTAAGTGCTTGTTTTTGATTTTCAGTTTTAGGCTGAGGTTTTTTGAAATTTTTCTTTGGCGATGACATTCAGTCTAGTGCGCACGCGTATACACGCACGCCAAGTGGAATTGTACATTATTTAGTGCTCAACCATGCTTTTAAAATATTTTAAAAGCTCTTTTTGTTTCACAATTAGTTGCCGAAAAAACTGTAAATCTTCCTCATAATCCTCAGGGTCATATGAGAGTGATTTGTTTAAAAAGAACTCCTTAATTTGCGCTAAAAAAGTTGTTGGAAACATTGCTTGTTGTTCCCCTAAAATACTGTTAAACACAGCCCAATCAGGCTCTTGCACCTTAGCTCAATAAAGCCGCGCTTTATCAATAAAAAGGGCAACATCAAACAACGGTGTTTGCAATGAAGCAAACAATTCATCTAAATCAGATTGCTTTAATTCGACTAAAAACTGCTTATCTAACAGTACGTAAGCGAAGATTTCAGCATGAAATACACGTTCCTTGGTCTCCTTTCAGTTGTTTTCGACTAAAAACTCCTTGGTAATGGCAACCGGCTGCGATTCATTTCTAAAACTATTATCAACCACACCAACCACTGATTCGTAAATCGGGGCTCTTTGGGTAAAAGTTGTAGTTTTATTAACTTTTACTTTGAGCTTTTGCTGCTGTAATTGTGTGAACAGTGTTTCCACATCACTGTATTGCAACAATTGCTGTAATTTCTGACACAAAAAGCGGCTAAAGCTAGTGTCAGCTGTTACTTTCTGGTTTTGTGCAATAATGTCGATAACTTTATTGACGGTAATCCGCTCATCAAAGCCCTTGGTTTTAAAGTAAGCCACTAAGTATTCCACGAGGTTTAAAACCCTGTGAGTCTTAGCTTTAACCGCATCACTACCTTCAGCTAAAAACAACTCATCCCAGTCTTTGTAAGCGGCTTGGTTTCAATCGACCACTTCCACAATAATGCCGTGTGCGTTTAACTCTTGTAATAGCTTAAAAGTAGCATCGTGACCCGCTGTATCATTATCGAGTGCCAAGACCACTGTTTCTAGCGCTTTTAGTTGCTGTTGAAAGGCAATAATGTGGCTTTCACTCAAGGCTAAACCCATTAAGCCCACTACATCACCAATCCCAGCGCTAGTAAGGGCAAAGACATCGAAGTAACCCTCAACTAAATACAGCTTTAGTGTGTTTTTATCAATCCGGTGGAAGTTAAATAACAATTCACTCTTCTTAAACCACTGGTGTTCTGCACTGTTTTTGTATTTAATCTTTTCCGTTTTTTGCAGTGCACGCCCTGAAAAACCTACTGGATTACCCTTAAGGTCATGGATCGGAATCATAATCCGGCTCTTAAAGGTTGCATAATGCGTTTGTTGGTTAAAAAATCCTAAAGCTTCTGGGAATTTTTCAGTAGCAAAACAGAGCTCTGCCTTTTCAATTGCCGGGCTAATTCAGGGATAGTTTAAGAGACTGGGTAGAAAATACTGGTCTTCTAAAGTAAACGCTAAACCCAACTGAAAGCGTTCAATTACTTGAGGACTTAACTTTCGTGTTTCAGTTAAGTAAGTCAAACCCTGCTCTTTGGGCTCTAGCTTTAACCGGGTTTGATAGTAATCGACTAGGGCACTGTGTAGATCCCAATAGCGCTTTAACTTTGGGTCAACTTCCTTAAGGTGAAAGTTAACAGCGTATTGTGAGTCAAGATTGAGAATACTAAGTGCCTTATTGAGCGCCGTTTTTCAATCTACCTGATCAATTTTTTGGATAAAAGCAATCCCATTACCGCCCACCTGACACGAGAAACACTTAAAGATGTTCTTTGCAACTGACACCGTCATCGAGGGGTTTTGGTCAGCATGAAAGGGACAGAGTGCCACAAAGTCCTTCCCCTTCTTTTTTAGCTTAATGGCATAGTGCTCCACTATGGGAGCTATTTTGATTTGCTGTTTGAGCTGATCTAGGCTAGTGGGACTGGTCATCGTGTTGGGCAAACCAAGCCTTGAGCTCCTTGATTGGGACACGCACTTGCTTGAGTGAATCCCGTTCTCTGATGGTAACAGCTTGGTCTTCTAAACTTTCAAAGTCAAAGGTAATGGCAAACTTAGTGCCAATGGCATCAGCTTTACGGTACCGTTTGCCAATACTACCGGCGCTTTCAAAGCCAATCCGTCAGTGGGTTTGACTCAGCGCTTCAAATAATGTTTGTGCTGTTTCTTTGAGTTTATTGACTAAGGGCAGCACTACAATTTGTTCTGGACATAAGTGAAAGGGTAAACGTAACACTTCGCGCATTTCCCCTTCCAACTGCTCTTGTTGATAGCTGCTAACAATCAAGGCATAAAACAAGCGCTCAATTCCCACCGAAGGCTCCACTACAGCTGGAATAAAGTGTTCGTTATTCTCTCCATCAAAGAAGTCTAAGGGCTTTTTGGCGTACTTTTGGTGTTGTTCTAAATCAAAGGTACCACGATCAGCTAAACCCCACAATTCCCTCAAGCCATGGGGAAACTGGAATAAAAAATCCACTGTTTTATTGGCATAGTGTGCTAATTCATTCTTGTCATACTCGTATTGTTTAACCAATGCTGGGTTTAATTGCAGTACCTTAAACAAAAAGTGAGCAATTGCTTGTTGTTGGCTTTCAAAAACGCTTAAACTGGCTTGAGGATTGCAGAACCACTCCATTTCAAACTGTTCAAATTCACGAACGCGAAACAAAAAGTTTCCCGGGGTCACTTCATTGCGAAAGCTCTTACCAAACTGGGCTACGCCAAACGGTAAAGGACGCTTTTTGAGTTGTAACAGCTGTTTAAAGTTAATGAAGATGCCCTGAGCTGTTTCCGGACGAAGGTAAACCAGGCGTTTTTCGCTGTTGACAACGCCAATTTCAGTTTGGAACAAGAGGTTAAAATCGCGTACTTCTGACCAATTATTGGCCTTACAGTTAGGACAGTTGACCTGCTTTGGGTTTCACTGGGTGGCGCTTTTAATTTGTTCATCCAAATGATCAACACGAAAACGCAACTTACAGCTCTTACAATCCACAAGAGCATCAGTGAAATTGGCTAAATGGCCACTAGCTTTTCAGATCTGTTCGTTGAGAATAATTGGCGTGTCAATCAACAATACATCCCGCTTATTTTTAATGAAGAAGTTGTACAGTGCCGTTTTAATTTGTTGCTTTAAAACGGCACCCAACGGACCGAAGTCCCAGCTGTTGGCAAGTCCATTGTAAAGCTCACTACTTTGAAAGACAAAGCCATAGCGCTTGAGGTATTGCACATACACTTCTTGGGAATATACTTGAGCCATAGTGTTAATTAATCTTAAAAATTAGCTTTTTCATCCAAACAAGATAATCCCCACGGCAACACTAACATTGAGTGAGTTGAGCTTGGGATTCATCGGAATTTTGACCTTTAAATCAGCGTTTTTTAACAGTAGTGCATTTAAGCCCTTATCTTCATTACCTACCAGTAAAATGCGCTTGTCAAAGTCTACTTGGCGGTAATCTTTTGGCTTTAAGCGCTCATCTAATGTTGTAGCGACCGTTCAAAAGCCGTGTTTTTGCAGGGTAGTAATGGCATAACTGAGGTTAGTAACCTGCACTAAGTTCTGGTAAAAGACAGTTCCCATACTGGTTCTAATAACGGTACTGTTAATGGGCGCCTGGTTATGCTTTTTAAAGATAATCCCATCCACTTCAGCTGCCATACAGGTGCGCAAGATGGCGCCAAAGTTATGCAGATCTTGGATCTCATCGAGCATTACCAGGGTACTGGTGGACTTGCTGTCTGTAATTTGGATTAACTGCTCAATTGTTTTGAGCATTTGGTTAGGGTTGATTACACAAACAAATTCCTGGTGGTTAATGTGGCGAAACTGCTGGTGAAACCACTGGCTGGAGTGCATTTCATACCGCAATCCCCGTTTCTTAATTAAGCCCAACAGCTTTTGGTGCTTATCAGATAAATTGACCTTTTTAATTTGCAGTTGGTTATCTAACGCTTCCAAAAAGGCCTTACTACCAAATAAAAAACTGGTTTCTTGCATCCGCTTCATTACTATAACAAACCTTTTTTAATTAGCTTTTGGCGCACTTCATCAGCCTTGTCTAACTGACCATTTTTGCGCAAACTAGCTCACTTTTTAATTAACTGTACATTGAGCTTAGTGTGGATCGACTTAAAGCCAATGCCCAAGATGTTTAAAGCTCACTCCAAATGGCTTAAATGTTCCTTTAAACCGCTCAAGTTTTGCTTGCTTACATCATGGTTAATTTGTTTGACTAACTTCCAAATGTGGGTAATGGCATTAGCAAAGTTGAGGTTATTGAGAAGTGCCTTAAACACCGGTTCAAACTGTTTGGGCGCGGGAATGGCGGATTGTTCAGAATACACAAACCAAGTGCGGCACACATTGACTGCCTTTTGGATCCTTTTAATATCACTGCAAGCTTGCTCTATTAAAGATTGGCTTAAATCCAACGGGTGGTAATAGTGTTTTTGATAGAACAATCAGCGCAAAATGCGAAAGTCATGGATTGTCAAAAAGTCAACTGCTAAAAGAAAGTTTTGCAGTGACTTGGACATCTTTTGGTTTTCAAACATTAAGTGGCCAATGTGCATCCAATGCTGCGTTAATGGTTTGTCATACAGTGCCATATGCATTGCGTTTTCGTTTTCATGATGGGGAAACTTCAAATCCACCCCACCACCATGAATTGTCAACTGGTCTTTAAAGCTGTAATCAATTAAAAAGGCACACTCCACGTGTCAACCCGGCCGACCCCAACCTCACGGACTGTTTCACTTTACCCCAGCGGTCGTTTGTTTTCACAAAACAAAGTCCAGTTTATTGCGTTTGTCAGTAGCTTCATCCTCAATTGTTTCCACAACACGGTTGGCTAAAATACCATACTGGGGTAATTGGCTTACACTAAAGTACGTGTTGCCCAAAGGCGAAACGTAAGCAAAGCCCATTTGGACTAACCGGGCAATGTAACCGGGCATTTTGTCAATTTTATCGGTGACTTGGAGGTGTTTAATCGGCAAAATGTTGAGCTTTTTCAACAAGCTTTTGTAAGCGGTGATTTGCTGTTTGCACAACTGCGCTTCACTAATTTCCTGTTGCTGCGCGATCTTGATAATCTTGTCGTCAATATCAGTGATGTTTTGGACAAACTGCACCTTGTACTGCGCTGCTTGCAACACCCTTTGTAGCACATCAAACACAATTAAGGGACGGGTATTGCCTAGGTGCAAATCATTGTAAACAGTCGGTCCACACAGATAAATGTTAATTTGCTTTTGTTCTAATACCACGCTCTGATTGCTCACCGTGTCAATGAGGGTAAATTTAGGTTCAAATTGGTTCATTAATAATGGGAATATTGAGGGCTTTAGCTTGTTCAAGTTTACGAGGTGTAGCCTTAATGCCAGCTAACACAAAATCAACTTTACTAGTGACGTTATTTGTAAAACGACAATCATACTTATTGGCGAGCAAGTTTTTAATGGTATCGCGACTAATTGGAAAGCTGCCAGTAATAACAAAACTCTTTTGAAAGTAAATGCTCGCGGTGTCAAAGTTAGTGTTGCTCTTTAAAGCATCCATTTGCACTCCAACTGCTTTAAGTGCAGCTAACAGCTGTAAGTTATTGTCATTGTGAAACCAGTTAAACAACGATTCTGCCACAGTCGGTCCCACATCAGGGAGTTCTAACAGTGTTTCCATACTGGCGGATTGTAAGGCATCGATGTTTTCAAAGTGCTTCGTTAAGCTTTTGGCTAACACTTGCCCCACATGTTTAATGCCAAGCCCAGTTAACAAGCGTGCCATCCCCTTGGTTTTGGACTGTTCGATGCTGCTGATTAGTTTATTGAACAGCACTTCACCAATTTTAAGTTCCAAGGCTAACACCGCGGCTTTGTGCTCCTTTAAGTGGTATAAATCAACGACGCTGCGCACTAAGTTATTTTCATACAGCTTACTAATGGTCTGGATACTTAGCCCCTGGATGTCCATAGCCGTTTTGGACACAAAGTAATTAATCGCTTCAAGGTTACGCTGGTCACAACGTTCATTGGTACAGTACTGATCTACCTCATCCTTATACTGCACTAACGGGGCTTGACATGCCGGACAGTGGGTTTGTTCGGGAATCGGTTGATTCGCTGCTGTTCTTAACGGTAAACACACCTCCAATACCTTAGGGATAATTTCCCCCGCTTTGTAAATTATCACTGTATCGTTTAAACGAATGTCCTTCTCCTTAATGTAGTCAAAGTTATGCAGTGTGGCTGCTGATACTACTGTCCCCTCTAAGTTAACTGGATCTAACTGCGCGGTATAGTTAATCTTGCCAGTGCGTCCCACTGTTACCAAGACACCACGCAGTTTGGTTTGGACAAACTTCGGTTTAAACTTGTACGCCAACGCCCAACGGGGAAACTTACTAGTAAACCCTAACTGGTCGTAAAAGTGAAAGTTATCTAGTTTAACCACTAGGCCATCGACTTGGAAGTTCAACTGATCACGTGTTTGGTCAAACTGATCGAGGTACTCAATTAATGCTTCGGGTCGATCAAAGCTAGCCACCGCTTTGGACACTGGAAAGTTTCACTGCTTTAACTGTGCTAACACGGCACTTTGACTCCTACAACCCAAAGTTAAGGCGTTGGGAACAAAGTAAAAGAAGGCCTTAAGTTTGCGCTTGGCACTCACCTCACTGTTGAGGTTCCGTAGTGTACCTGCTGCTAAATTGCGGGCATTGGCAAACGGTTTTGCTAAATTCTCGTTAATTTGCGCAAATGTTTTTTTATCGACAAAGATCTCACCGCGGATTTCCACCTGTTTGTGAAAAGGAATCGTTAGGGGAATGCTCTTAATCGTTTTAACATTACTCAAAACATCCTCACCCACAAGTCCATCACCGCGGGTTAAAGCCCGAGTTAACAATCCGTTTTCGTAAGTTAAAGAGATGCTTACACCATCAATTTTGGGTTCCACCACAAAGCGGTTTTGCACTCCAGTTAAGTTGTTAATGTTGGCAATAAAACTAGCAATTGTTGCTGTGTCGAAGGCATTGTCTAGGGAAAGCATTGGATTTTGGTGAGGAAACTTATTAAATGCTTCCACTACAATTCCCCCAACACGTTGGGTTGGTGAATCGGGTTGGATTAACTCAGGATGGGCTTGCTCCAACGCTTGCAGCTGTTTGTATAACTGGTCGTATTCAAAGTCACTGACCAGCGGATCGTCCAAAACATAGTAATGGTAATCATAGCGTTTTAACTGTTCAACTATTGCTCGAATTTGTGCGACCTTTGCCATAAATTAATAAAAAGCCCTGATTAGCTTACAATGCCAACCAGGTGCTTTTAGGAAAATTTAATATGGAGCAGATAACGGGAATCGAACCCGCATCTTAACCTTGGCAAGGTTATGTTCTGCCATTGAACTATATCTGCGCAGCTCATAATTTTAGTATATTAAAGCTAATGGAAACGCAAAACCAAATAGAAACGTTGCGCTATATTTTTAATCAGCTCAACAACCAAGACAAACCACAAATAATTTGGTTTTCCGGTGAAGGTGAGGATGAAAAAATTAACTTTTTAATCCGTTTGGACAATTATTTCCAACCTACCTTTGTCCAAGACCTTACTATTAACTTTCTCCCCGCTTTTGTTAAGCGAAACAAGAAAAATCCACCCAATACTTTAGCTAAGGGTAACTTTGTCAACATAGCTAATAAGTTATTGGCAGTGTTAGCGCGTTCCTTGAGCTGAAAGCAGTTAAACAAACCCCAGCAAAAATGGTTGTTATGGTTACTAGTGCCCTTTTTGCTTTTGCGCCAACTATGGTTAAAAAAGAAGGTAAGCAAGATCTTTCAGTTTGTCAATGAACGTGGCATCTTGAGCTTCATTAAGGAGCAGTGACCGATTTTAACGACACTCGTTACCGTGGGTACTACCTTAGGTACACCCATCTTTTCGATTACCATTTCCCAGCAAAAAGCAATTTTGGAAAACGCTGGTCATGGTGCGTTTGTCTTTTTAGTAATCTTTTCGGTGTTTGCAATTGCGCTGGGTTTAGTCTCATCGTTAATCTTTTTAGTCTCATCGTTATTTTCAATTCGTCAAAAAAAGAGCTTACAACAGCTCCACCAAATTCTCTCTCGGTTAATTAACAAGTACTTCTGTTTGGCTAACAGTGAACAAAATCAAACCGGGCGGTACCAACTGAAAAATACCGGGGTGTGCTTCTTTTATGGCTTTGACTTTGAGGAAAAGGAATACATAACCCAAGCGATGAACCTACTGTTGCTCTTGAAGCAAACGAACTGCTTTGTGTTAGTCGGTTGTAAAGAGAGCAACATGTTGTTAATTAAAAACAAGGTGGAACCCGACATTAACCTTAAACAAAGTAGTCTGTACCTTGATTTAAAGAGTCAAATTAGTCCGTTGGCACAAATTAGTAAGTACAACCTCTTGTTTGAAGAGTTGGCCCTGGATGCTGACATGTTTTACTTAGAGGACTTCTTCGCCTTGTTAAAGACTCCGCGGCAAATTGTCAACTTTTTGTTTCGTATAAAACAAAACTTAAAGGAGTTTCACCAACCCCAAACGTTGTGGTTTGACTATTTGGCCTTGTGAGCCTTAGTAATTGCGACTGACTTTGAATTTAACAATGTCTTGTGGTCATTTAACGACTATTTAAGTTTAACGAACAAGCAAAAGGAAGATTATGCATCAGTTAACCTCACCGCCTTTTTTAACCGTAGTTTAAAGAACCATAAGGACAACAGCCTGCTCTTTAAACCAGAATTGTTCAACACTCACGCTTATATTCCTGAAACTTACACTCAAGTTACCTTAGAAAACATTGATTCAGACAAACGTGCGCAACTAGTGGCATTGAACTGGTTTTCACAGCAAAAGTTTAGCGATTTTATAGAAGAGAAAATTAACTTTTGACAAACTCAGCAGGCGGAAAACAAGGTGTTTTACCTCACTTTGGGCGAGCGGATCTTCTTTTTAGTATTAGTTAACAAGAAGTTCAAACAAATTAAACTAGAAGCAGCGTTGAAGTACCTCAACTAACTTCCCTTGGTGTAATGGATAACACGACTGAGTCCTAATCAGTAAATGGAGGTTCGATTCCTCTAGGGAAGGCCAAATTATCACAGAGACCTAGTTAACTACTAGGTCTTTTGTGTATATAAATTAAATTAAAATGATTAACCGCTAAGGGGGTGTAGTTTAGTGGCAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCACCTAGTGATGCATTACAGTTACAGCTTTAAAGAATACATCGAACGGTTTGCCAAAAAGGTTAATTCGATTGACAGCGAGTACTTCGAATTTTCCTCTTACATAGAGCGCATGCGCACTGTGTTTGGTTTACTGATTGCACTGATATGCTTTAGTAACGTTCTCTGTTTTCTTTTTATTGCAACTTGGTTTAGCACAAAGGGATTTGGTCAACACTACCGGGCGTTAATATTTACCTTGTTTATTCCCTTTGTCACTTCACTTTTAGCCAACATTATCTTCATTAACCTTAATCGCGCTTTTCGCGAATACTTTAAGATCAGCAGTAAAAGCCGTTCTTTTTTGGTAATCTGTGCCTTCTCCTCATTACCGATTGTGAACATCTGGCTAATGTTGTGGTGAGTAGCGATGATTAAGCGCTTTACTAGTAACTATGCCTTTGCTATCTTTGATAAGTACAACGGTTTGACTAGTGGTGTGTTTATCTTTGACTTTGCTGATAATGTCAATTTTGAGGGTAAACTAGTGAGCTTTGACAATACCAAGGACACCAACCGTGACTTTGTCCACTTTTACAGTGAGGCCAAACTTAAACGCGATAAAATTACGCTACAAACGAATCCAATTCCACACGAAAGGATGTATGTTAACCGCATGTACTATCAGCAAAAGCTCAGTATGGGTGCTAACCAAAACAGCCCTTCAACGGCATTTGCTAACTTAAAGCGGTATGTGGAACATAAACAGCAAAAAATTATCAAGATTAAGCAGTTTATCTTGACCTAAAAAAGCAATAAATATATAATCTAAGCCGTTGCTTTGTAAAACCAATGAAAAAAGATTTTCATTTTCCAAGCCAATCCGTTAGCTTCAAGTGTGCTTCTTGCAGCAATAGTTTCACCATTGAATCAACCCTAAAACAAAAAGAAATTACGATTGATATTTGTGGTAAATGTCACCCGTTTTACATTGGTGAATTAACCAAACAAACAGTCCACGGTCGTGCGGAAAAGCTTTCTGGAAAGTTCAATGCTGGCAAAGCCTTTTTAGAGAATAAAACTCCTAAAAAAGCGAAAGGCAAAACAGAAGAATATACCAAACACCGTTCTTTAAACGAACTGTAGGTGGAGTTTGACAAGCAGTTCTTTTCTAGTGTTGAAAAGATTGTTGAACTTGCTGAACAACTCGAAAAGGACTTAAATAAACCCGATTTGACCTTTGAACAGATCAAAGCGATCAACAAGGAACTGAAACATAAACAACCCTTAGTTGTTAAATTCAAAGAATTTAAACGGCTCATAGATCAAGCTTTGGAAGCGGAGGCCATTTTGGAAAACAACGAGCTCAAGGAGCTACATGATGAGGCCAAAAAAGAGCTGGAACGGGTGCGCAGTGTTGTCCCTGAATACGAAGAAGCGTTAAAACTGTTGTTATTACCAATTGATGAGAATAACCAGAAAAACGTGATTGTAGAGCTTCGTCCCGCTGCTGGTGGGGATGAATCGTGCATCTTTTTAGCTGATTTATTCAATATGTACCGCAACTTTTGTACTAACAAGGGTTGAAAGTTGCAAATTAACGAAATGATCCCATCCAGTGTCGGGTTAAACTTCGTTTCCTTTGAAGTTAATGGTGTCGATGTGTTTGCCAAGCTCAAGTTTGAATCTGGCGTACACCGGGTACAACGCGTACCCGCTACTGAATCCAAGGGGAGAGTACACACTTCAACCGTTACGGTTGCTGTTTTACCACAACTAGAAGCGGTTGAAGTTCATATTAACCCAGCTGATCTTAGAGTAGATACCTACCGTGCTTCGGGTGCTGGGGGTCAACACGTTAACCGGACTGAAAGTGCAGTGCGGATTACCCATTTACCAACTGGCATAGTAGTTTCGTGTCAAGAGGGTAAATCACAATTTACTAACCGTGATACGGCAATGAAGATGCTACGTGCTAAACTGTGGGAAAAAGCGCAGAACGAACAGTTATCAACCCAAGCAGGTTTGCGTAAAAGTCAGGTTGGGTCGGGTGATCGTGCCGAAAAGATCCGCACATATAACTATCCCCAAAACCGGGTCACAGACCACCGCATTAAACTTACCGTCAATAAGCTTAACACGATTATTTTAGGTGACCTTGATGAAATTATTGAAGCGTTGCAGGCAGATGAAAAGAAACAACAACTAGAGAACTTTTTCAGTTAATGAACTTGTATGAACTTTTCTTGAACCAAAAGCTGCTGTATGGCACCGATCCGCACTTTAACGGTGTTTTTCTCACCCTATTAGAAAAGTTTGGTCTGCACTTTAAGGATTTAACAGCACTGTGAAAGCACGCTAAAACAATTACTGACTTTGACGAACAGGGAATTGTTAATGCTTTAAAAGCTTACTTTGTCGATCAACTACCCTTACCCTATATTACCGGGAGTGTTAAGCTAGGTTCATTAACTTTTAAAACTCAACCTGGCGTCTTTATTCCTCGTGCCGACAGTTTAGCGCTTTTAAAGGTAGTCAAAGCACAAAACCTTAAAACCGCGGTTGACCTTTGCTGTGGAAGCGGTACGTTAGCAATTGCGCTAAAAAAGCGTTTTCCTCACTTAAATGTTTATGGTAGTGACCTCAACCCCCAAGCATTGCAGTTAGCGGCGCAAAATGCCCGTTTGAATATGGTTGAAGTGCAGTGAATTGAAGCTGATTTTTTAGCTGCTCTAGCACAGGTAAACACACCTATTGATCTCATTATTACCAATCCACCCTATTTAAACGAAAGTCAGTTAGATCAAACTTTAAACCACGAACCGCGCAACAGTTTAGTGGCTGATGGTAACGGAATTTTGTTTTACCAAAAGCTGTATAACTTTTTATTGGGTAACCGCCAAGTTAAACAAGTGATACTGGAGTGCTCACCAACCCAGAAAAAAGAGTTTTTAGCATTATTTAGCATTTTTAAAACTAGTGAAATTTACACAAGCCACAAGCAATTCATCGGTTTGAGTATTGACAACACTAAACTACCAGTCTTAAAAATTGCGCAAACCAAGCAAATTAAAGCACTGCTAGACAAGGGGATGACGGCCATTATCCCCACCGACACGCAAATAGGATTAATGAGTTATTGCCAACAAGATTTAGACCACATTAAACAACGCGATCCCAACAAGCACTACGTCCAGTTTTTAGCACCTAGTCAAATTAACCAGTTACCCAAACAATTACAAAAGCTAGCCAAACTGTTTTGACCAGGTGCTTACACCTTTATTGTAGATGGACAGAGCTATCGTCTACCCAACAGCCCACAGCTGTTAAAGCTCTTAAAAACGGTTGGTTTAATTTACTGTACCAGTGCTAATCAAGCTAAACAAAAACCATTTGGCAAGTTAAGCGCTTACCAGAACGATCCTTACTGAGTGCAGCAGAACTGTTTTATTGTGCAAAACAGCTTTAAAAGTAATAACGAACCGTCCTTAATTTACAACTTGGATACGAAGCAAATTGTTCGTGGTAGTTCAACCCAATTACAACGTTTCCAAGCGTTACTAGCAAAACACAAATTAAGACACTAAAAGTTTCAAATTTGACTGCTAAAAAGCACTATTAACGGATTATTGGAGCTATCCAACTTATTTTAACTTTAAAAATAAAGAATTTGCACCATTGCTGTATAATTTCCCATCTTTTTTGGTGTTTTGTAAGATGAAATTTAAGTATGGTGCTACCCTTTTTAGTGGCTTTTTAGGGCTTTCAGCGATCCTAGCTGCTTGTGGTGCAAAGGGTAAATTTGACCAAGTTGATGATGGCAAAATTGTGTTAGCCTCTTCATTAACTTCTAAAAATGCCGCTAATGCTCTACAAGCTGTGGTGGAAAAATACAACCAGGTTAAGGGTGGCAATGATTATCCTATTGAAATTACCCAAATTACCGGTGGTTATGACGGTGGACGTGGTAACTTACAAACCAAACTTAGCGTTAAAGACAAAACGACCTTTTACAACCTCATCTTAAACTATCCCGACTTAGTTTCCACCCTAGGACGTGTTGGGATGGAACTGCCACTAGACAACGTTGATCTCTCCAAGTTATCACCTAACTTTTTGAAGTTCAACGAACGCATTAGCGGTGTAGCCAAAAAGGCCAATTACGCCATTCCCGTATCACTTTCAACTGAAGTTTTAGCTTTAAATGGACCGGTATTACACTATATCTTGAACAGCGCGAAGGGTGGTAATGAAACAGCTGTAACACAAAGAACTGCTAGCACAAACCAACAAAATACACTTCAAAATTCGTTAAAAATTAACGAAAGCGACCCCATAACCAAAGAGCTTTGAGAAAAAATTCAAAAGGCTGCTGAAACTAATGGAAAGGCTAAAGAACAAGGTAAAAGTAAGAAACAAGGCACAGAGAAAACTACCACTCTAACATTACTTAAAACTGAATCTAGTCAACAATCTAACACCACTAAAGATGGTAAAGCTACAAGCGATGAAGCAATTAAGAAAACTTGAGGTGATTACCAAAAGGTGGAGAATGGCCTTAAGGGTTATACCTTTAAAGCTAGTGTCTTTGAAAACTGGCATGAACTGCTCGATTTTTCCGCAAGGATAGCTAGATCATTTACTAATATTCACACCAAAGAAACCAAAAAGGGAACGGACATTCAAGGTGTACTTGGGATTGACAGTACACCTAACGCCTTATTTACTTCAGTATTTGCTGCAGGTGGTGGTAACTATGACAACTTCTTTTATAAGGTGCAAGATGGTCGGGCTGACTTTAGTAACTTTAAAAATAAGGAAACCTCTTACCAAAACTTACAAAAAGTATTTAGCGATTATAAGGAGTTAATTGATAAAAACGGTTTGTTTGTCAATAAGGGTGGCTCTTACTCTTCTAACTTCCAGAAGTTCCACCAATTAGCTTACAGTATTTCTTCCACTTCAGGTTTCTTTTATTCGTTTGCTGGTAACAGTGCTAAACGCTTAAAGTTTGATGACGGTAACTTTATTGAGTTTCCGCGCTTCACTCAACCAGTTAAAGCGCCTAATAAAACAACTCAAAGTGGTGGAGAAAATAACAATAAAAGTGAAAATAATCTTCTTGGAACTTTTGAAATAGAAGACAAAAGCAAAAAGCGCGAAAGCGTTGAATCTAGTGAAAAATCAAGTTCTGGGACTCAATCCAAAAGCAAGAAAGGTAAAAGTATGAAAACCATTTACCTTTATAAAGCACAAATTCCAGAAGGAAAAAACAAAGGTGATAATGCCATCTTAATTACTAACCAAACTGTTATTGATAAACTAGAAAAAGCTGCTAAAGAAGACGGTAAAAGTGGAACTCAAGTTTCAACTGTTGCAAGTAAATCAACAACTAAAGAGAACCAAGAAAACAAAATAATTGGTTACACATCGACTGATAACCTTCGTGAAGACAATAAGAACATTTTCGATATCGATGATCTTAACGATGAAAAATATGACCGTAAGATTATTGTTAGTGCTACTGAAGAAGTATTGGAACAATCTAACACTTTACAGAGTGAAGAAGCAGTTGTTCTACCGGCACTTGCTAAATATAAAGCTAGTGATTCCACAAAAATAGCAATCACTCAAGAACCTAACCTAATTGGTATCCATGCTAACGAAAAGGAAAATGAGGAAACACGTAAGTTTGTTAATTGGTTTTTAAACAGTGAGCAAACTTGGGAATCCAAGAAATACAGTAAAAATAAGCAAAACTCCAACGAAAAGAGAACTCCTGCACAATTCTTTGCTGATTCCGCGTCTTACATTCTACCCTTAAAGAAAACCTTCGATAATGCCAACAAAAAAGAAGATCAAAGTAAGGCCAATAACAAGACTAATAGCCAAAGAACTAACACCTATATCGAAAAGGCATTAGAAATTTTTAAAGGAATTAGCCAAAACCAAATAACAAGTTACAGTGATCCAAGCGACTTTAGAAGTGGTCGCTTCCGCGATGGTATTGGTTCTAACTTTAATGCAGTAGTAAATTCCAAAGTGGATTTCAATAAATTTATTAATAACTTTATTGCTAGTCTAGGTTTTGACATCTAATAAAGCTCTTGTTGAAACAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGAGATTTCTAGGGGCAAAGCAATTACCAAAGAATACATAAGAGCTAACCCTGGTGAATATCCTGTTTATTCTGGTTCAACATTAAATGATGGTGAAATAGGTCGCATAGATGAATGCGAATTTGATGGTGAATATGTAACTTGAACAATTGATGGTTATGCTGGCATAGTTTTTTACCGCAATGAGAGGTTTAATGCCTCTCAACATTGTGGTGTCTTAAAGGTAAAAAGCAATGAAATTTGCCCTAAATTTTTGGCATATGCATTGGGGATGGAAGCGCCTAAACATGTTAATAATGCTTGTGTTATTCCTAATCTAACTTTAAAAAAGATGAGAGAAATAGAACTAGATTTTCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATAACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGAACTACAAATGGTTATGCTGGAGTAGTTTTTTATCGTAATGGCAAGTTTAATGCATCACAAGATTGCGGTGTTTTAAAGTTTAAAAATAAAAAAATCTGCACCAAATTCCTATCCTTTTTATTAAAGATAGAAGCACCAAAATTCGTTCATAATCTAGCTTCAAGACCAAAACTAAGCCAAAAAGTAATGGCTGAAATCGAACTCTCTTTTCCACCTCTAGAAATTTAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCTGAAGTCGAAATGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTGCAAGAACAAAAAACTCAATTAGAGCAAATTATGTAATGCTTAATAAATAGTTTCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACCACACCGCTCAATGGGGGGCTGGATGTCGTGCGGGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGACTCTAATAACCAAGATACGAGTCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTAGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTATACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAACAACCCCGCTCCCAAGGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGGATGGTTGGAAGTGGTGCGGCTGGAAGTGCTTCTAGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAAATCGCTCTTGAGATCAGCACCTGTCAGTGTTCCACCAAGCAGTACAAGTAATCAAACTTTAAGCTTATCTAACCCCGCTCCTGTGGGCCCACAAGCGGTTGTAAGCCAACCCGCGGGGGGTGCTACGGCAGCAGTGTCCGTCAATCGCACAGCGAGTGACACCGCCACCTTTAGCAAGTACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATTGTTCCGGGGTTGGAAAAATGAGGTGGTAACAACGGTACGGGTGTAGTGGCTAGCCGACGGGATGCTACTTCCACTAACCTGCCCCATGCGGCAGGTGCTTCCCAAACGGGTTTGGGAACTGGTTCGCGGGGCGAACCAGCTTTAATCGCAACGTCACAGCGTGCCGTCACGGTGGTTGCTGGCCCCCTTCGTGCGGGCAATAGCAGTGAAACTGATGCCCTACCGAATGTCATCACCCAGCTCTATCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGAGTTCCGACCGGGTACCGAGTCTTTGGTATTGAGTTGTCGGGGAGGACCAGGAATCGGGCAAAGCGACCTGATGAGCGAAAACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCATTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCGACCTACAACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGACAAGGCTTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGTTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTTCAACAAAAGTCACTCTATCTGCTCCCACTAAATAAATTGACAGGGTTTTATAAAAAAGCCAAAGAGTTTGAATATTTTTGTAATTTGGGTTTTTAATACCTTCTAACTATTAATAGGAACTTTTCTTATTAATAAGAACTTTTTTGAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAGCACAAAAACCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCACTGCTGCAAGTAGTAGTTTTCTCAACCTCTTTCAGAAAGGAGTCGCTTATTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATTACATGATGAAAAAGACCAAAAAAGGCACTTTTTCGGGATGATACATCATTAATCCAGAAAACAACAGTGTGGAATTCTCTGGCAGTTTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACAGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAAATAAGATCGATAAGCTTGAAATTAAAGTTGATAAGCTAAAGAAGAAAGTCGATAAGCTGGAAGTTAAGGTTGATAAACTAGTCGAAACTGTTAATGCTCAAGGAGAAGATCTTAACAACTTCAAAGTTGAAGTGAGAGGAACACTTCAAAGCCAAGGCGAAACCCTGCAATTAATTTTGCAAACGCTACAAGACATGAGTAAGCGGCTTGATTCTGTTGAAGGACGTTTAGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTGGACAAAATCGATCCGCTAAAATAGTCTTAGGCTAATAACAAAAGTGCCAGCAATTAATCTATAAAGGTTAGTCGCTGGTATTTTCATTTATTTGAAATAAATAAAAAAGCCATCCTATCAAGGAGAAAGACAGACAGCTAATGAATTGAAGTCAATTACTTAATTAAATATTCCAAGTAAATGAAGAGATTAATATCTGATGAATAGCCTTCTGTATTATTAGCAAAGGTACTTAAAAATTTGTTCACACTTTTTTCACTAACATCATCTTTGCCTTTTAATGAAAAGCTAAGTGAAGCGCTAAACTCAATTCACTCTAAATTTCTTGTAATTTCTTTTCCATTAACTTTAATTTTTTCTTCTTTAGTAAGAAATTTTTCTGTAGTAAATTGTTTTCCTGGATCAGAATTAGATTTAATTTTAAGGAAAAGATCAAAATAATGCTTGTCACCTTTCTTAGCAGTGAAATCACCTGAAGAATAATCTCCAAACCAATTAAAGGTATAGCTCAACTTAGCTTCAATTGACAAATCCTTAAAACTTTGGTATGTCATCTGGAAATATTCAATCTTTCGCTCCATCGTAAAAGGATCCTTGCTTGCGGGATAAATTACATCGTCAAAATCAAAATCTGGAATACCTAATTCGCTCTTTTCCGCACTTTGTTTTCAGGCGTCTAACAACAATTTTGTTGTATCTTTCGGATTAACTTTGTAAGACTTCTTTAGAGAAGTTATCATGTTCTTTTTGCCTTCTGTAGTTTTAATTGCTTTATACACATCAATGTCTTTACCGGTGGCTTCTTTAATAAGATTTCTAAGATCTGCTTGGATACCAGTACAGGCGCTTAAAACTGTTCCACATAACAAAAATAAAGAACCAAAGGAAACCTTTGACAAAAACTTCTTTTTGCGCATCAACAGTAAAAATAACTCTTTAATTTTGCTTTTTTTTTTTTTGTAAAAAGCCTTTTTGGGATAAGAACTTGAATTGGAATTTCGGGTTGAACATTAATTAAAACACTGAAATTTCCAATCATTTATTAACAACTATCTACTGATAAAGGTTTTGATCTGAGTAGTTCTAATAATTGCTAATTGTTGGCATTTTTATTCATTTAATGCTTTCTAAAAACTTTTTGCAATTTTGTTGGCACTTCTCAATAAGTCGGTCAATTTATTTAGTGGAAGAGCAAATGCAAATCTTTAATTTTTTGAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAAAAGAAAGGAACATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCAATGAAAAACTTTAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGACGGTACTTGTGAATTCATTATGAAAATTAAAACTGTAAATGAAGTTGCTGACACTGAGATAACAAATAAGGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTAACTTTTTGGTCTAAAAAGACATAAATTTACCAACGGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTTCCCCCACGCTCACCAGGTTTAGTACCGGGTTCAACCTCGCGGTGTCGGTGCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGCACAGGTATGGGAATAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTGGGTGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGACCCACCCTCGACCCAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTTCAAACAAACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGCAGTGGCCAGCAGGGTGGTTCTACAACGGGTGGTTCAGCGGGCAATCCCGGCTCGTTAAAGCAGGATAAGATTAGTAAATCAGGTCAGAACTTAACCACGCAGGACGGCGCGCCCCAGTCTAATTCGACGACGGAATCCGCGTCGAATTATGATCACCTCCCCCCAACCTCACCCCCACATCCGATTTACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTCTTCCTCCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCAGATGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCTTACACCGACTTAAAGTCGGACCAAACCAAGCTCAACCTCCCCGCTTATGGTGAAGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCGCAAGACGTTGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGTTGGGCGGGTGGCTGGTCACCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGGCTCCAGTTAACGGGCTTGGATGCAAGTGATGCGACGCAGCGCGCCCTCATTTGGGCCAAGCAGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGCTTGGGCCGGGTCGAGAGTGTGTGGGATTTAAAGGGGGTGTGACAAGATCAAGCTCAGGCGGCCGCGCAAGCAGCTACCACCACCGCCGCAACAGGGGACGCCTTACCGGAGCACCCCAATGCCCTCGCGTACCAAATTAGCTCCACCGACAAGGATTCGTACAAGGCTTCCACTCAAAGCTCCGGTCAAACCAATTCCCAAAACACCTCCCCCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAACACGACCCAACTCGACCAGGGCTTAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAAGCCAAACCCCAATCCCTCAAAAAAACCACACCGGTGTTTGGGACGAATAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGACAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAATTACCCAGTGGGGGTGGGGGGAATAGTAGTGAGGATATTAAAAGTGTGCAAGACACTCCTATTTATGATCTATATTCATTAGTATATTCTTTTTAAATTGTTCCCTCACTTTATTCATATGAACCACCGCCTCCCTCGCGACGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGAACGTATCGTAACACGAGCTTTTCGTCCCTCCCCCTCACGGGTGAAAACCCCGGGGCGTGGGCCTTAGTGCGCGACAACACCGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCACCGCCACCACCTTTGCGTTACGCCGATACGACCTCGCCGGGCGTTGTACGACCTCGACTTTTCGAAGTTAAACCCGCAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGAACGCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCGCGCAACCGTACCGTCATTGACCGCGTTGATCACTTGGATCCCAAGGTGATGGTGGATAATTACTGTTAACAATTTTTTAGAAAAAGGCAGTTTTTGAAGTTTTTAATTTGTAAAATTTCATTTTTTAAAAATGCCAAATCCTGTTAGATTTGTTTACCGTGTTGATTTGAGAAGCCCTGAAGAAATTTTTGAACATGGCTTTTCAACTTTAGGTGATGTGAGAAATTTCTTTGAACACATTCTCTCCACTAATTTTGGTAGAAGCTATTTTATTTCCACTTCAGAAACACCCACAGCAGCTATTCGCTTCTTTGGTAGCTGGTTACGGGAATATGTACCAGAGCACCCCAGAAGGGCTTACTTATATGAAATTCGTGCCGACCAACACTTTTACAATGCCCGCGCCACTGGGGAGAACTTGTTAGATTTAATGCGTCAAAGACAAGTAGTATTTGACTCTGGTGATCGAGAAATGGCACAAATGGGAATTAGAGCTTTACGCACTTCCTTTGCGTATCAACGTGAATGGTTTACCGATGGTCCAATTGCAGCAGCTAATGTCCGTAGTGCTTGACTAGTAGATGCTGTTCCCGTTGAACCTGGTCATGCTCACCACCCGGCTGGTCGTGTTGTAGAGACTACTAGAATTAATGAACCGGAAATGCACAACCCTCATTATCAAGAGCTGCAAACCCAAGCCAATGATCAACCATGATTGCCAACACCAGGAATAGCTACTCCTGTACATTTATCAATTCCCCAAGCAGCTTCCGTTGCTGATGTTTCGGAAGGTACTTCCGCTTCGCTATCGTTTGCGTGCCCTGATTGAAGTCCACCTTCTAGTAATGGTGAAAATCCGCTAGACAAATGCATTGCGGAAAAGATTGATAACTATAACCTACAATCCTTACCACAGTACGCTAGCAGTGTAAAGGAACTGGAAGATACACCAGTATACCTAAGGGGAATTAAAACGCAAAAAACCTTTATGTTACAAGCAGATCCGCAAAATAACAATGTCTTTTTGGTCGAAGTAAACCCCAAACAAAAGTCCAGCTTTCCCCAAACCATCTTCTTTTGGGATGTTTATCAACGAATTTGTCTCAAGGATTTAACTGGTGCACAAATCAGTCTTTCGCTTACTGCCTTTACTACTCAGTATGCTGGTCAGCTCAAAGTGCACCTTAGTGTTAGCGCGGTTAATGCCGTGAACCAAAAGTGAAAAATGACACCGCAAGACAGTGCAATAACTCAGTTTCGGGTCTCCTCTGAACTGTTAGGTCAAACTGAAAATGGCTTGTTCTGAAATACCAAGAGTGGTGGTTCACAACACGATTTGTATGTATGTCCTTTGAAAAATCCACCTAGTGATTTGGAAGAATTACAAATAATTGTTGATGAATGTACTACCCATGCGCAGTTTGTTACTATGCGTGCAGCTAGCACCTTCTTTGTTGATGTTCAGCTAGGCTGGTATTGAAGGGGTTATTACTATACCCCACAATTAAGTGGTTGATCTTATCAGATGAAAACACCAGATGGACAGATATTCTATGATCTAAAAACTTCGAAAATCTTCTTTGTCCAGGACAACCAAAACGTGTTCTTTCTCCATAATAAACTCAACAAACAAACTGGTTACAGCTGGGATTGAGTAGAATGGCTAAAACATGACATGAATGAGGACAAAGACGAAAACTTTAAATGGTACTTTTCGCGTGATGACCTTACCATTCCTTCCGTTGAAGGGCTTAACTTCCGCCACATTCGCTGTTACGCTGACAACCAGCAGTTAAAGGTGATCATAAGCGGTTCACGTTGGGGCGGTTGGTACTCCACTTACGATAAAGTTGAAAGTAATGTCGAAGATAAGATTTTGGTCAAAGATGGTTTTGATCGCTTTTAGCGATTAAGCTTTAACGTCACTGTTTTGCTCTAATGTTAGAAGCAAAGATCTTGTCGACAAACTCGTTGCGCTTGCGGTAACGACGTACTACTAAATAAGTTACAGCACCAGCAGCTACTACACTAGCTATAGCAGCCACAGTTAAACCAGCTACCAACGGATCTACGTGGGTTAAGAAGTTGAAACTGCCACCATTTGCATTCAATTCAACGGTAAAGCCATCTAGTGATCTTTCACCTTGAACTAGTTGTTGGCTAGACAATGAGCGTGGTTTGAAGAGGTCAAATGAACCATTAGCACCAACTTGGTTTTCGTACTTAACTTGTAAGAACTCCTTCAATTGTCTTTGGAAGGTACTTTGAATTGGTGCATAAGCATCATTAATAAATGAGCTGTCGGTAAACAATGCCCCTACAATCATGTTGTACTCATACGGATTGAGTCCAGAGAAATCAAACACAAGTGTTTTCTGCTCCTTATTATTTCCCACACTTCTATTTTCTATTTTGTAAGGAATATCAACCGTAGTGCTTTGTTTTGGGTTGAATGCATTCTTAGTTAAGGCAATTCTTAGATTACCACCTTCAGCATTATTGTCAGTTTGGGCACCGCCATCACTTACCATAACTGTGTAAGATTGTGCTTCCTGTGCCAATTTGTTGACATCATTTGGGTCATTCAAACCTAAAGAGCCATTTTGTTCAAGCGAGAAGTCGGCTTTTAAGTCTAGCTTGATGCCATCCTTTTCAACATCAAACTTAATTTCGTCCTTTAAGAAAACCTTCTTGTTAGTTAGTCTCTCTACATAACCAACCACCGCACCACTAGCAGCTTGACTTAAAACACCACGTTTAGAACTCTCGTTGGACTTCAAGAAGTGCGCTACGATCTCTCTGTTTTTGGTTGAAAGAGAAGTGGTTGTTTGGAAGAAGTTTTGCGGTAATTTTCCCCTTCAATCACTAGTGTTGAAATACAGTTTAGATTTTTGTCTTGCTTGAATTACAGCGTCCCTTGAGGCATTATCGACGTATATACCGTTGTTGTCGAACCTAAGTGCATCGAGTGAGTAGAACTTATCCTTTGTTTCAAAACGGATAATGTAAGTACTTTGCGCGTTCTTGTCTAAACCTTCTAGGTAATAGAAATAGTTTCTAGGGTTAGCAATACGACGTTGGTTACCAAGGTTGTTAGCGTTAGTTACATCTACTACCACATAGAAGTGACCATTGCTTTCATTTCCGTTCAAGTTAATATTGTTCTTAGTTTGAAAGTTTTGCAGTGCAGTTTTGAGTTGAGTATCTTTAGTATCAGTGCTGCCTAAAACATCTTGAACTTTGCGTACGTTATCAAAGTCGAGGTTAGTGCTAATACCGTTGCTGGTAAGGTAATCGTTAATGCTCTTGTTGAAACTCTTGTCATCAATTTCACGTAACTGATCATTTTGTGCTTTTCAAACAATACTTCTTGTCTGTAAGTTTGCCCGTTCTCAACTAGCACTTTGGAAATCTCTTCTTCAGTTAATATCTGAAGACTAGCTTGTTGTCTTCCTTCTTAAGTAGTGTTTTAGCTACAGATACAACCTTATCTTCTTTCACTTCCAAGCGATCAAGGAAGTCATTATCACGTCAGCCACCATCTTGAAAACCAATTGCATTAACAATAATTTCAGCTTTGTTTTCGTTTGCTAAAGCATTGAGCTTGGTCTTCATTGTTTTCAATTGCTAAGAACTGGAAGTTTTGTCCATCCCACACTTTAACTTCCTTAGTTACTTTTTGTCCTAGGGTAACGTTAGCGTAAATGGCCATGGAAAAGTAAGAAGCTACTAACTTTAAGAATTGATCTCAGTCCTTCGGTTCTTGGTTTAAATTAAGACCTAATTCCTTATTTGCTTCTTGATACCACTTAACTCAACCGGTTGAGTTAATTGGAGTACTTACTTTATTGGTATCTTTATCTAGGCTACTAATGTATTCTTTTACCTTTAGGTAATCAACTTGCTTGTTTTTAATTTGCGTTAAAACGCTGTTAATGCCAGCTTTTTCACTTTCGTTACTTTTGCTCTCTAAACGTTGTTCTAAATCCTTTACTTGTTCAGAAAGGTTGTAAACGTTAGTGATTGCATTAACAAAAACTGGATTTAAAACAAAATCTAAGGTAGGTGCTTGAGTTAAAACACCGTTTCTAGTTGAATTTAGATTAGCGTTTGTTTTAGGGATCAACAGCTTATCTAAGTTAAAGTTTTTGTAACGGACAAAACTAAAGCTTTGGTTTTGTAGTTTGTAATTTCTGTTTTCAAACTTAAAGTTAGTTCCAGTGGTGTTAGTGTCAATCACCTCTTTCACTGCACTACCTAACCCAACATTTAGAGTTTCATCCTTGCGTACGTATGCTTTAAGTTGACTTTCAGCTGCACTTTGATAGTTGGCCTGTGTACTCTTAGCGTTAGCAATAAAGTCAGCTGTTTTTATCGATTGCTGCTGACCATTGCTGTCGGTGTAAAAAAGCTTGGTTCAAGGATCTAACTTAACAAAGAAAGTAATGCCGTGATTAGTAAAGCTCTTTAAAGGACGGTCGGAATAATGAATTGTTGCAGGGTAATCTAATGGATCACTGTTAGCACCTAAGAAAGTGGTTTCAAAGTTTACGTTAGGTTTTTCAATGAAGTTACGATCTTTGTTTGCAAGTCACTAGTTTCTAGTAGTTGTAAAACCAGATTGACGGAACCATAAGAATACTCCTTTCTCTGAGTGGAGGTTGTTGACGTTTTGTAACAGCTTTTCTTTAATGCTACCCCAAACGGTTTTGGAAGTTTCGTTTGTTGCGGGGTCAGATTTTTTTAAATCTGCTTGACGGTGAAGTTCACCCCTTTCAATGAGCACTATCTTTTGGTGGGCAAAGTCAAGGTCAAACCAAGCAGTTCATCTCCATCGATCATCATTGGTTCCAGCTTTTTTTGCACCCCGGTAGTGGGTTTTGTAATACTTGAACTTATCATACCCTGCTCGGTATTTTGTTTCATGTCGAAACGGCGATTTCCAACCGTTATTATTAACAGTGCCCACTAAACCAAAACTCATATCTGACTTAGTTTAGATGTTTACTTCTTTTATTCCTGGAAACTTGTTACTTAATTTAGAAAAGGAAGATCCACCAACAAGCCAACCAAAACCAGCGAGTCCAGCACACAACAGCAACGAATAATTTCGTAAACGCTTTATCCTCATACGTGTAAATTTATAACAAAAAAACGCCCCTTGGGGAACGTTTTTAAACAAGTTTAATTATTAGTCATTTTTATTGAATATTAAATCTTCATTTATTTAATGCTTAAAGATTAATTAACTTTTGCACTTGCTTAAGAAGTCCATTTTGACAAAGAAAAATCCCAAAAGGATGGCAATAAAGCTCACGCTTTTGGGATCTTCCTTGTGGTTTAAACCATTTTTTTAGCTAAGTCTACTTAACTTTTCTTGCTTTGAGCATTTGTTCTCAGAAGTGCTTACGACTCTTACGTCAGCGGTGTAAGACAACACCACTAGCTATTATTGTTGAAAGTAATGCAGCACTACCAGTGAAAGTAATTGCTATTAATGGTCAGTTAATCTTATTGATGTCTTCTTTATTGAAAATGTTTTTGTGTAGATCAAAGGTGAAGTTATCAATTTGTTTTTGGTTATTTGCCAATTGTACCTTGGTCAATAAAGGTAGTTGACCAGTCTTAATTGGTGCTTGGTATTTGTGTTCTAAAACGGTTTTGAGCTGCTTTCTAAATTCTGTTTGCAATGGAGCGTAGGCATTCTTAATAAAGGAGCTGTCGGTAAACAACAGACTAACAACTGTGTTGTATTCGAAGTTGTTTAGGTTAGAGAAGTCAAACACCAAGTTTTTCTGCTCTCTAACCGTACCATCCTTGTTAGTTTTGGATTTCTTGGTAACAATCCAGTAAGGCAGTTTAATAACGGTACTTTGTTCAGGGTTGGTGGTTAAAGTAATCTTGAAGATGTTTTTGTCTGATTCTGGATCATCACCTTTTTCTTCTGAAACCAAGACATTGTAACCCTTAGCTTCTTCTACAATTTGGTTAATTACACTTGGGTCTTTAACCTCTAAATTACCCTTCTTGTCTAAGGTAAAGTTCGATGTTAAACGTAGCTTTAAATTATTTTTGTCTTCCTTATTAAAGCTCACCCTTTCCTTTAAAAAGAGCTTTTTACCAGTCATTCGCTCAACATAGCCTAAGATCGAACCACTGACATTTTCACTCAAAAAACCCTCATCATTCTCAGCGTTAGGTGTAGATAGTTTAGCTGAATTGTCAGCACTTGCTGTAGGCAAAGTAAGTTCAGCGTTGGTGAGGTTAGCCTTCAAAGCAGCGTTCCAGTTATGAGTATCGAGATAAAGGTTTTGGTTTTGCTTGGCTTGGATAATTGCATCCTTGGACACATTTTTCACATAAAGGCCACGACTATCAACCGCTAGTGATTCGAGTGAATACAGCTTTTGTTTGTTTTCAAAGCGCACTAAGTACGAACTTTGTGCTCCTTTGTCCAACCCTTCAATATAGTAAAAGTAACTCTTAGCGTTATTTGCCCGGCGTTGGTTACCCAAGTCATTGGCATTAGTTACATCCACTACTACATAAAACTGACCAGTGTATGGGTTAGATCGTAGGTTTAAACCATACTTGTTTGCCGTACTTTGAACGCTAGCGAGCTTCGGATCATCACTTTTGCGGGCGTTTAACATCGCATCAACTTTATGAACAGCATTTACTTTAATTTCAGTTTCAGAGATGCCTTGTACCTTTAAATAGTTGTTAATGTCCTTTTGGAAGCGTTCGCGCTCATCGTTCAAATCAGTGCTTTGTACTACAATACTCTTGGTTTGTGAGTTTTGTTTGTCCCAGCTAGCTTGTTTAAAGTTTTGTCGGTAATGGTACTTATAAGAAATTTCGTTGGCTGCTTTTTCTATTTTGCTAGCATCACCGTTAGCAATTTTAGTTTTACTACTCTTAGGGGTTAGAGCTACCTTATTGATAAAACTAGCATCACGTAAACCGCCTTCATCTTGAAAACCAATGGCGCTTACAGCAATCTCAGCGCGGTTTTCATTTGCTAGTTGTTCTGATTGTTTTTCAGTGTTTTCAATCGGGATGAACTCAAACTTAGCACCATCCCAAACCTTCATCCGTCTTTCCTTTGCGCCGTTTAATTTGACGTTAGCAAAAATGTCCTTAGAGAAGTAAGAAGCAATTAAGCTGGAAAGTTGTGTTCAGGTATTAACATCATCGTTCAAGTTTAAACCAAACTCTTTTTTAGCATCAGCATACCATTGTGCAGTATTTTCACCCTTGGATTCAATACTACCTGGATTTTGGCTTCAATTTTTCAGCTTGCCAATAAAATCAGCTACTTGAATGTAGTCAACTTCCTTATTTTTGATTTGTTCTATAACCTTATTGATTGCTGCTTTGTCACTTTCATTGTAAGGTTGATCAGACTTTAAATGCTCCTTTAAATCAAGTGTTTGCGCAAACAGATTGTGAATGTTGACAATGGCGTTTGTCAAAACGGGGTTAAAGTCAAAGGACAGTGTTGGTTGTTTGACAAAAACACCATTCTTAATTGCCGTAGCATCCTCGTTGGTTTTTGGCACTAGTAACTTATCGAGCTTAAAGTTATTGTAAGTGTCAATGCTAAAGGTCGAATCCTTAAGTTTGTACTTTAGCTGTTGGTTGGAGGCTATATCACTCTTAGTGGTGTAAGTTTGACGTACAGCACTACCTAAACCTAAGTCAATACTGCGAGTTTTTCTAACAAGCGTTTTAATCTGGTTTTTATTAACCCATTGGTAATTAGTCTTGGTACTCTTTGCTTTTCTAATGTAATCACTTACAGTAATTAATCTTTCCTGACCGTTTTCCTTGTAAAACAGCTTGCTGTAAGGTTCTAGCTTTAAAAAGAAGCGCGTGTCAGTGGTATGACTACCTCAATCAGGACTGTGCCAATTGGAGTTACCACCTTCAGAAGTGATGTTGGTGGTAAAGGCATTGGGGTTATTGTTGGTATCCCAAAAATCGGTAAAAAAGCCACTGTTAGCCCAGCCCTTAGTACCCTTGGTGTTAAAACCAGTTTGGTTGAACCAGATGAAGATTCCCTTATCTGGATCTAACCGCTCAGCACTGTAGAGAATCTTCGAGCGAATATCACCCCATAAAGGTTTGTTTTTATCCCATTTTCTGGGAGTTGGTTCTGGAACACGCTTGTCAATATGATATTCATCATTTTCCACCAAACCTATGCGTTGGTTGGCATAACCCAAATCAAACCAAGCCACCCACTTTCAGTAATAAGGGTTACTTGCTGTCCTTTCGCGGTTAGAGTACTTGTAATATGTTTGGTTCCAGCTAAAGTGAACGTTTTTGTCGTATTTGTTCTCGTGCCGGAACGCTCAAGTGCGACCGTTATCAGTGTGTCAACCCACTAAACCAAAGCTAGCGCCAGAGTTGGTATACACCTTTACATCTGGTAGTTCACCGTTCTGAATGGAAAGCTGAGCAGTACGCTCAATTTGCTTAAGTGCTTCTTGAGCGTAAAGGCTTTCACTAATGTAGTTATTAACGTTGTTTAAGTAGACCTCTGTCTTTTTACCGTTATTTCACTTTAAATCAAAGCGCTGGGTAATAGCTGGACTTTTACTCTTTAAGATTGAAAGCACATCGCCTTTACCCTTAAGGTAGCGCATGTGAACGCCACCATCGCTTGATAGTGTTTCATTGTTACTTTTAACAACGCTAGTGAATGCTGAACTTGCAACGAATCAACCAAAACTTCCTAATGCAAAACCAAAAAGTAAGAATCGGTACTGTAAACGTTTCATGCTTTATTCTAGCACTTTAAAAATATTTTTGACAATCCTTCTGCGCGTGTATTAAAGCAAATGCTGAACGAAATTGACAAAAGACGCACGGTTTGAACTCAAAAAGCTTTTTATGGCGTGGTATTTCACCAAGCATTTCATTCATTTTACCCTCTAAATGAAGAGTTACTTTGCTCTGCTTATCTCAATATAGTTAAAGTAATGTGTATAAACGTAGCTAAAATAAAAAACCAACTGAGAGCAGTTGGTTGGAATCAATAATTTGGCGCGCCCAATAGGACTTGAACCCATAACCTTCTGGTCCGAAGCCAGACACTCTATCCAATTGAGCTATGGGCGCTTCTTATAGTTAATTCTATTAAATCTCTGAGAACTGCGCAAATCTTTATTAAAATTAGCTGACTTCAACACAGTCGCTCTAATACATGTCAAAGGATAAAAAAAATAAGGTTGAACAGCTTGAACCAGTAGACCTTTTTGAGCGGACTAAGCTAGAGGACACCCAAGTTTTAAATGATGTTGAACTAGATGACATCAAGAAACTAGAAGAGTTGAAAAAAGAATTGGAAAACACCTTTGATCCACGTACCAGAATTGAAATTAAACGTGAAATCAAAGAGTTGGAACGTAAACTAAGAAGGAACCGTTAGTGAATGTTTGTTAACCTCCACACTAACTCTTACTATAACTTCCTCAACTCCACACTTTCACCGCAAAAGCTAGTTGACCTAGCTGTTCAAGACCAACAGGTAGCTGTTTGTTTAACCGATCCCAACTTATTTGGTGCTACCGAGTTTTTTTTGGCCTGTCAAAAAGCGCACATTAAACCCTTAATTGGGTTGAGTGTTACTGTTCGACATTACGAACAAAACGTTAATTTACTAGTAATTGCCCAAACCAACCGGGGTTATCAGAACCTAATGTGCTTAGCGTTAGTTAAAGACCAACCAGATTTGCAATTAGAACCCTTTTTGGATGGTAATGTGGTAATTATTTGCACACAAACTGAACTGCGCCTCAACACCGCAAACCCCGTTTACTTAGCACATGGTTTGAGTGGTCGTTATCCTAAAATTGCAGTAACACAAAAACCAGTTAAGTGTCAAAACACCAATAAGGACTTAACACTGCTCTTAACACTTAAGCAAATTAGTCAAATTAACAGCGAACACTTCCAGCCACTGGAATGAAAATTAAGCCGTAGTTTAAACGAAATCCAATTGGATCCACCATTGTTGCAACAACTCCGGCACCAACCTTTTTTAAGCCAAAAGGCAGCACAGCAAATTTTTAGCGAAGAGGAATTGGGAAATCTGCAAAAGCTAGTGGAGCAAAGTCAATGAGATTTAACCAAACTCAAAGCGTCATCGTTACAAGTGTCACACAACGATGCACAAATGTTAAGCGAGCAGTGTCAACTAGCCTTAACTGAATTTTTAAAGCTCAATCCGCAATTAAACAAACAGCTCTATGAAGAGCGGTTAGCCAAAGAATTGGAGATAATTAACTCACTCCACTTTGCTGGTTACTTTTTAGTGGTGAGCGACTTAGTTCAGTTTGCCCATAATAACGATATCCTCATCGGTCCTGGTCGAGGATCTGCAGTGGGTTCTTTAGTGGCCTTTCTGCTCAAAATTACCCAAATTGATCCAGTAGCCAACAATCTCATTTTTGAGCGCTTTATTTCCCGTCACCGCCAGGGCTTACCTGACATTGACATTGACATTATGGAAACTAAACGTGATTTAGTAATTGACTATGTCATGCAAAAATATGGCCGGGAACAGTGTGCACAAATAGTTACTTTCCAAAAATTTAAGACCCGCTCTGCGCTGCGTGATGTCGGTAAGGTATTTAAACACATTGAGGGTGCTGAAGATTTACTGGGTAAACTACCAAAAGACAAAAGCTTATTGGAGCTTGATGTAAACGGTGTTACTGATCCGGTTTTACAACTTAGTTTAAAGAGCTTTCGCTTGTTGTGGGAGGTGGCGCGTGAAATTATTAACTTTCCGCGCCAACCAAGCATTCACGCTTCTGGTGTTGTGATTGTCACTGAACCTTTAATTACCACGATCCCTTTAATGGTGGGTAATAACAACAACTACGTCACCCAAGTGTCGATGGACTGGTTAGAATGGTACAACCTCAATAAGTTTGACTTATTGGGTTTAATTAACCTCACCATGATCCATGAGGTTGTGCAAGCGGTAAAACCCAAGGAAGTTAGCGTACAGCAATTCCTCCAACAAATACCGCTAGATGATGAAGCTACTTTTACTAACCTCACTAACCAAGCCACTTTAGGTGTTTTTCAACTAGAGTCGTTTGGGATGAAAAAGGTGCTCAAACAAATTAAACCGCACAACCTCCATGATTTAGCAATTGTTTTAGCACTTTACCGCCCCGGTCCACAGGACAACATTAATACCTTTATTGCTAACCGTAACTTAGGGTTTGACACATCAGACATCGATCCGCGAATTTTGCCAATTCTCAAAGAAACTTACGGAGTGTTAATCTTTCAAGAACAGGTTATTAACATCGCCAAAACTGTTGCTAACTATTCATTGGAAACCGCTGATAGCTTTCGCCGTGCCATTTCCAAAAAAAACTTGCAAGTAATTCAGGACAACATGCGCAGCTTTTATGAAGGCGCGCTAGCCAATAACTTTAGCTTAAAAGCAGCGACTACTATCTTTAATTACATCCAGCGCTTTGCTGGATATGGTTTTAACTTGTCCCACGCCTTGGGCTATGCTTTATTGAGTTACTGAACAGCATGATTGAAGACACATTACTTTGAGCAATTTAACCTGTGGTGATTAAACCACGAACAGGGCAAAAAGGAAAAGCAAAAACAGCTCTTAAACGAGTTTATTAGCAGTGGTTATGAAATTTGCCCACCACTAATCAATAAGGCTAAAAGTGATTTTAGTGTTCAAGATAAAAAGCTGTATTTAGGCTTTAAGTTAATCAATGGTATTGGTGACAAGCAAGCCCATGCCTTGGAACATGTTCAGGAAGTTTTAAAGCAAAACCCCAATTTATCCTTAATAGCTACCGTTAATTTGTGTTTGAGCAAAACGGTAGGGGGTTTAGAACTTAAAGACATTACATTGCTCCAACAAGCAGGTTGTTTTAATTGCTTTAATTACACGGTTGACTTTAATTTGGCCAAAAGCTTTTGGGTGCAAAGTAACCATGAATTATTTCCAAAAATACCACTAGATCAACCACCCGTAATTAACTGAAAGAGCTTTGGTTTTTAATTTATGAAAAATGCAATTTTAATTGATGGTAACTCACTAGCCTACCGTGCTTACTTCGCTACTTGACAACAGGTGGAGTTTGCCAAACTACACAACTTACCCTTTAACAATGCGATTCGCACAATGTTAATGATGTGCTGAAACTTACTGCAGTCAAAACAGTATGACTATGGTGTTATTAGCTTTGATACTAAAGCGCCCACTTTTCGTGATCAACTTTACAGTGAATACAAAAGTAAGCGCAGTAAAACACCGAGTGAACTGTTAGTCCAAATTCCTGTTGTTAAAGAATCACTGCGTCATTTAGGTTTTTTAGTGTGTGAACAAGACGGGTTTGAGGCAGATGATCTAATTGGTAGTTATGCGAGGTTAATGACACAAAACAATGTGGCAGTAGACATCTACAGCTCTGACCGTGATTTACTACAGTTAGTCGATAGTATGACTAGTGTTTGGCTTTGTGTAAAGGGCACAAAAGAGATGAAGGAATATAACACTGACAACTTTGCTGAGCAGTTTTTTGGCTTAACACCAAAACAAGTGATTGAATATAAAGGTTTGGTAGGGGATAACAGTGATAACCTCACTGGCATTAAGGGAATTGGTCCGAAAAAAGGGATTGATCTACTCAAACAATACGGTACCATTGACAATATTTTTGCTAACTTTGACAAGCTATCCAAAGCATTGCAAACGATTCTCCAAGGCCAAATTGACACTGCTAAGAAATTCAGCTTTTTAGCGAGCATTAAAACAGATATTAAACTTAATGACGATATTGTCCACGCTGCTTTAAAACCAATCGATAAACAAGCTTTATTAGAGCTTTTAGATAAATATGGCATTAAAGCACTAGCGCAAAAATTTAGTCAACTGTAATGCCTGAACTACCTGAAGTTGCCACTGTAATTACCGAACTTAAGAGCTGTGTTTTAAACAAACCTGTCAAGCAAGTTAAGGTGCACTTAGACAAGGTGTTGAAAAATACTAATGTCAAGCAGCTTAACGATGCGTTAGTCAACCACAGCTTTGTGGACATTAAGCGCCGCGGCAAGTACATTATTTTTTGTTTAAGTAACGGTTTGTTTTTAGTGTCACACTTACGCATGGAAGGCAAATATTTCTTTGAAGCTAAAGGCAGTCAGTTTGACTTAAACCATGTTTTAGTCGAGTTTTTATTCCAAGATGGTGATCAACTTAACTATCATGATACGCGTCAATTTGGTACCTTTCACTTGTTCAATAGATATCAGTTTGAAAACGCACGTGAATTGAACAAATTAGCACTCGATCCCTTAGATCAGGAATTTAACCATCAAGCGATCTTTAACAAAGGCCATAAGTCCAACAAGAAGATTAAAACCTTTATTTTAGATCAGACCAACATTAGCGGTATTGGCAATATTTACGCTGATGAAATTTTGTTTGCAAGTAAGATTCACCCTGAAACTTTAGCTAAAAATTTGAACTTATCGCAGTATCAGCTAATTTGTCAAAATGCAACTGATATTTTAAAGAAAGCAGTTGAAATGAAGGGTACTACCATTGGCACTTTTACCTTTAAAAAAGATCATACCGGTGGTTACCAACACTTCTTGAAAATTCATGGCAAAAAGGGGAAGCAATGTCAAAGCTGTAACACCACTATTATTAAGAAAAAGATTAATGGTCGGGGCAGCTATATTTGTGAAAAGTGTCAAATACAGCGTTAATGGTTAACTGTTCCAGTTAATGCCAGAATTATGGCTTTATAAAAAAGAGCTTAAATATCTTAAACTTAAGCTCTTTTTGATCACTTAAATTAACCATAAAGATTGTTTTTAATCCTTAGATTAATCCACGCTTGTTAGAAAAAATATGAAAAACAAAATTAAATACGTTTACTCCGACCTGGATGGCACGATTGTGAGCTGAAATCCTAAAAACCAGTTTACCCATCAGGGCAAAACTTACAAAAATCTCCATGAAGTTAGTCACGCTACCGTAACTGCTTTTAAACAATTACAAGCTCAAGGTATCAAAATTGGAATTGTGACTGGCCGTGATTACTGTCGCGTTCGTTGGTTAGAAAAGTACCTAAACACTGATCTACCAACAATTACCCTCGATGGCGCCATTATTTACTTCCGCGATGAAATTATTCGGCAAGAAGTGCTTGATAAGGAATTTATCCATGGCATTAACCAGATTGTCAAACGTTACCCTACAGCAGCCTTTAAGCTCAACATGGGTTGAGGTAACTATTACACCTGCAATCCTTCTTTAATCTTTGAAGGTGACCATGCTTACCGTGAGCACTTTAATGCTGACAGTAAGTTTTATCGCAAAGAGATCGACAACACGGTTGATTGAGATATAAATAACATGAAAGTAAATCAGGTTTACTTTGACACATTTACTTGCCCTGAACCAATGATCCAAGAGCTCGATAACTTAGTTGAAAAAAGTGATGTAACCGCCAAAAGCTACCGCCATTCACTTTACATTATTAAAAAAGGGGTATCGAAGGCTAGTGCACTACAAAACCTCCAACGAGACTTTTTAGTGGAAATGAAGCCTGCTAACACGATTGTCTTTGGTGATGGGGATAATGACATTGAAATGATGCAGTGAGCTGATCACAGTGTGTCGTTAACTGGTAGTGATCCCGAGTGTTATAAGTTAGCTAAGTACCACACCGACAGCGTTGACGATGATGGGATTGCCAAATGGATTAATAAAAATCTGTTATGTTAATCGCTGTTGTAGGCAAAGCTGGAGTTGGTAAAACCACCGTTTTGCAATACATCGCTGACTATTTTCATTTTCCCGTTTTTTTTGCTGATCGGTTTATCCATCAACAATACGCAAATGGTCAAGCTGGTTATGCAATTGTAAAACAGCAATTTGGTGCTCAGTTTGTCAATCATGAAGCAGTTGATCGCAAACAGTTAGCACAATACGTTTTTAATCAACCTGATGAGTTAAAGCGTTTGTCCAACTTAACCAAACCCCTAGTACAAGAGTGACTCAATCAATTAAAAGCGCAATTCCAAGACAAAATTGCACTTGTGGAAATTGCGGTGATGCTCAATTACTGAAACGATTACCGCCCTTTCTTTGATGAAGTAATCCAAATTGAACGTGATGCTAAGATAGTAAAACAAGCACTAAAAGCACGTGGCGTTGATGTAGAGCAGGTGCAAAAGTTAATTGCTGATCCGACTTACCCAATTCTTACAGTAATCAATAACAGCACTGTAGCTGAGTGTGCTTTACACGTTACTCAATTTTTAGAAAGCATTGCAAAAAGTGATAAATGCCATCATGGTCATTATCAAACTCCGAAATAAAGGTCGCGTTTTGTTTTGCTAAATCACTACCGTTAGCCATTGCGACAGAGTACTTGACAGCATTAAAAATTTCCACATCATTGTCCCCATCGCCAAAGACCATGGTCATTTGGGGATCTAAACCGGTTTGCTGTTCTAATGCTTTGAGACCGTATGCCTTATTGACATCTTTGTGCATAATGTCAAGCGCAAAGGTCATAGAACTGACATAGCTAATGTTTTCAATCGCATCGAGATGTTTGGTTATTTTACTAATGCGACCCCTGTCTTCAGTGATTAAAAGAATCTTGGTAATGTTTTGTGTGGCAAAATCAAACTTGCTAGCATCAGTTAACGGATTAATGGTAAAGTTATTTTGTGTAATGTCAGGTTCTACAATTTTGCTTTGAGTAGTGCGTAACCGTTTGCAAGGTATACCGGGTTTTTCAAAACCAAACACCTTTTTATCAGTGTAAATGCAAAAGCTTTCTTCCAATTGCAACAGTAAACGCATAATGGGTACCACACTTTGGGGTGCTAACTGGGTCATATGCAGCGCTTTTTCCCGAGCAAAGTCATAAATTAAAGCACCGTTAGAGGAAATAACCGGTAGATCAGGTTGAATAAACTGAGTGGTATTGCGGATTAAGATATGACTGCGCCCAGTCGCAAAGGTAATTTTAAACCCCTGCTTTTGCAACTCTTTGAGAAAAGTAACAGTTTGTGGTAGTGGTTCATGACCCCAAGACAATAAAGTACCATCGAGGTCAAAAATTAAGTTTTGTACTTTTGGTTTCATAGCTATTCGGATTTTAAAAGAACATTAATAATACGATTAGGGACAAAAACAACACGCGCTAAGGGTTGGTCGAACAACGCTTGGAATTCCGGATCTGTCTTAAAGGCTTTTAAGACAGTCTGTTCATCAACCCCTTTAGCAAATTCCTTGGTACCTTTGAAGCGACCGTTGACCGAAAGAATAATTTTAACCTTATCGGTTTCAAGTACGCTGTTATCAACATTTGGTAGTTGAGCATGAACAATAAACGGCTCCATCCCTAGTTTAGCGTTTAATTCTTCCGCTAAATAAGGTGCAAAAAACGATAACACCGTCAAAAAGCCCTTTGCATAAGCCAGGGTTAATTGTTTTGTTTTGTAAAGCAGGTTTAAAAAGATCATCATTTGGCTAATTACCAAATTGAGTTCTTGTTTCTCTAAGTGCTCGTAACTACGCTTTAAAAAGAGGTTATAACCATAAACAGTTTCTTGATCTACCGTTTCTTTAATAATGTTTTCCTTGTGAAAGAAATTGTGCACCCGATCAAGTCAACGCCGCATTCCGTTTAACCCATCATCCAATCAGGTCAACGCCGCGCTAATCGGCCCCATAAACATCAAGTAAAGTCGCAAGGCATCAGCACCATGAGAATCAATTAGTGGCGTAGGGTTGATTGTGTTGCCCTTGGATTTGGACATCTTTTTGCCATCTGGTCCCAATACCATGCCCTGGTTAATTAACTTTTTAAAGGGCTCTTTAGTGGTGACTATTCCCTTTTGGTACAAGAATTGGTGCCAAAAGCGCGCGTACAAGAGGTGCAACACGGCATGTTCGGCACCACCCACATACAGATCCACTGGTAGGTAACGCTCAAAAAGATCTTTCGCTTCCTTGGAGTCAATTGGCCAAAAGTTTTCGTTATTAATTAACATCAAATAACCCAAGTAATACCAACAAGAACCAGCCCACTGGGGCATAGTGCTCGTTTCCCGAGTAAAAGTTTGATTACCTTGTTTCACCTTAGCCCACTCCTGGTTTCGTCACAATGGTGGGTTAGTCGAACCATCAGGTTGATAGTTAGCCAAAGCTGGTAGGGTAACGGGGAGCTCTTTAACTAAATGGGGTTGGTTGTTTTCGTCAAACAAAATCGGAAAAGGTTCACCCCAGTAACGTTGACGGCTAAAAATCCAGTCACGGAGTTTGTACACCGTTGTTTTTTTAGCTTTTTGACGCTCGACTAACATTTGCGTAATTTGGGTGTTAGCAGTTTGGGAGTTGAGCCCGCTAAACTGCCCGGAGTGTTGCAAGCGTTCCTGTTTATCAATGACGGTAATAATCGGTAGCTTTTGCTTCCGCGCGAAGAAGTTATCGTTTTCGTTATGCGCTGGCACACCCATCACAGCATCCGTACCATAACCTTCCACTACATAGTTAGCTACATACAACGGAATGGCAGTATTAGTTGCTGGATTAATTGCTTTAAGTTTTAAATCAACCCCGTCATGGAGCGTCTCAGGTTGCTTTACAGTGGTGGTTTTCTGTGCTTGTTTTTGGAGAAACTGTGCTATCTTGGGGTTGGTTTTAGCTACCTCTTTTACTAAGGGATGGCTAGTGGAAAGCGCTAAAAAGGTTACACCAAAAATAGTTTGCACCTTAGTGGTAAAAATGGTAATCGTTTCCTTGGAGTTTTCTATGTCAAACGTTACCTCTACCCCTTCACTTTTACCAATCCAGTTGGTTTGCATTTCCTTAATAGATTGATGTCAATCCAGCTCAGCCAAACCATTGAGTAAACTTTCAGCAAAAGCAGTTATTTTCAACACCCATTGCTTCATTTTTCGCTTGGTTACCGGGAACTCGCCACGCTCACTCACCGCATTACCATTACTGTCAATCAACACCTCTTCGTTGGCTAAAACGGTGCCCAATTCAGCACACCAGTTGACATCAATCTCCTTCACTTCAGCCAAACCGGCTTCAAATAACTGACCAAAGATCCACTGTGTGTACTGGTAATAGCGTGGATCAGTTGTCTTTAAAGACAGCTGGTAGTCATAATCAAAGCCAAAGGCCGTTAACTGCTCAATGAAATTAGTAATGTTTTTGTCAGTTCAGTCACCAGGATGCTTATTGGAGGCAATCGCGTATTGTTCTGCTGGTAACCCAAAAGCATCAAAGCCAATCGGGTGAATCACATTAAAACCCTTCGCTTTATGATAACGACTCAAAACATCGGTAATGGTGTAAGCGCGCACATGTCCCAGGTGTAAACCAGCCGCTGAAGGATAAGGGAACATGTCCAAGATATAGTATTTGGGCTTAGTTTTATCATCAAAAAAACGGTTAACTTGCTTCGTTTTTCACAATTGCAACCATTTCTCTTCCAGTAAGTTGTGGTTATACATCTAGTTTTTTTTGAACGAGCGCTTAATTAAATACTTAATTAAATGGTAGAACAGGTCAGCTATAATGGCTACAGCAATCCCAAAAAGTAAACCAAAACCAACTGGAATAAAGAAGTGCACCAGGGTTTGGAACAGTTTTTGCTGTTGCTCTACAGTGCCTTTAAATCATTGATTAATATTGGGAATAATTTGCACAATTAGCCCACTAGCATCCTTTTCAGCTAGTAAATTAGCCCACTCCTTAGTATTGTAGGTATTGAGCCAAAAAGAGCCAATAAAAATAATAAAACCAACCGCGACAAAGAAGAGGAAAATCGCTAGTTTAATGAGCCGTTTAAGCAGCATGGTAAGTGGAAATTAAGCTAATTAAATTATAAAGCTAGATAATTTCCCAGTCTAAGTTTTTTGACTTTAATACCTCTAGTACTTGTTTGGTGATGGATTCTACTGATTGGTTAGCGTTTACTATGCAATGGCTAATACCAAAGTCATAACAAACGTTTTTAATAAATTCACCATAAATCTTGTACAGGTTCTTAAAGTAGTCCTTATTTTTGGCAAAGTTATCAATTTCTACTTTACGGTTACGTTGAAACAATCTTTGTTCAAACAACTCCCAATCACCTTCTAAGATAACGTACAAAGCGGGAATACCATGTTTAAAGATTAGTTCTTTAACAATCCCATTCCACAAGTGGTTATAGTAAGTAAAGATGGAAGGACGGTGGATGTTTTGCTTGGCGAACAATCAATCTTCAAAGATCGAGCGGTCAAAGATAGTTGGTGTTTTATTGTTACATTCCTTGCGGTATTTATTAAAGCGGTTTAAGGTAAAGTACAGTTGAAATAAAGGGGCGTAGAGCAACTCGTCGTTACGCTCATACATTTTGGCTAACAATAAATCAGCTAGCTGGTCTTGTTCTTCCAGTTCGTAAATCACCTTAGAACCCTTTAAGTGTTTAGCTAAACTTTCAGCTATCGTCGTTTTACCAAAAGCAATCATGCCACCAACCACAACGGCGTTAGCAATCTTAGCTGGTTGAAATTTGGGTTTTAAGGGAGTCTTCATAATACTTAGAACGTGGTTACAGTGCAAAGTATTATGCAATAAAAAACCATCACACTCATTAAATAGTCTTAATGCTGCTCCATTTCTGGCCTGACATGATTCGACCGTAATGATGTGACGGCTCTAAATTCTAACACTTAACTGCTTTTAATCAATTAATTTGCTTACGCGCTTAATCGAATTACGCTTCGGCTGGGTCGCGCCGCGAGGTAGATCTTTATTGGTTGTTGGTGGAGTTTCTTCCACTTCCATGTCATCATCCATATCATCGAGTGTATAGCTAATAACTTGGGTTTGCGGGATAACAGCCTCTTGAGGACGGTGGAATTGCTGTGAAGTGTCAGCTAGGCTAACGGTATGGGTCTGACCATTAACTTGCGCGTTAGTAATTTCACCTAACTTTTGTTCCAAGCGGTTTTTGAGTTTGTTGTACTGCTTGACCAAATCGTTGTTAGCGCGCTGAAAGTCACGCTTTTCACTGGTAAGTGAATTGTTAATTTCCTGAATCCGGGCTAAATCGACTTGCAAGTCACTCACCATCTTTTCGTACTTTTGGTTTTTAAACAACACTAGTTTGTGCTGCTTTTGCAACGCCTCATTTTCATCAACCGCACTTTGCAAGTAAGTTTGCAGTTGGGTAATCTCACTGTTCTTTTCGTTTAACTTACTTGTAAAGATGGCCTGGATTTCCTCATCACGCCTGTGAATGGCATCAGTACACTGCTGCTCGTACTCCTTCAACGCTTCTTCCAGGGTTTTGTTGTAGTGTTCCTCAAGCTTGTGGTTGTACTCCTTGGCTTCATTGAGCTGCTTTTGATACACCTCTAGTTGCCGTTCCTTTTCTTTAAATAGCTGCTGCATCTGGATAATTTCCTCATCACGTTGACCTAACTGGATTAACAGCTCACTGTTTTGTTGGCTCTGGCGTTTAATTTCCCCTTCATACCGTACTTTGAGCTGTTCTTGCAACACCAAGATCTTCTTTAAGCGATCCTTCTCTGATTGCTTTTCGGGGAGAAATTTCTGTGCTAAATACAGCAATGTTTGGTTCCAGTCAAGGTGTGCAGGGTTGGTTTGGCGTAACTGCACATAAGCCTGACGCGCTTGCTCTAGGAAGTCCTGGAGCTGTTTTTGACTCGCTAGTGGAAACTTTGAAAGAAAGAAGTTAATTGTCTCTCTTCTTTGAAAGTCATCAAAATCACGGTAATACTCAAAGTTAGTCATTTAAGGCCTTTTCACGGAAAAAGATAATTACAGCATCAGGATTAGTAAAGTCCAAGTCACAGTGGTACTTGACATACTGCCCAACACTGTTAAAGATTGCTAACACAAAACTAGTAAACAAAAAGAAGGGGATTTTGCTCGAATAACACGCATAAAAGTGCTTAATCTTAGCACGCATAGTACTTTTAAAAGTTTCTACATCGCTAAAATCACGCGGATTGAGCAAATAATCCTTGACATCACTAACCCGTTCCACCACCCAGTCATACGGAAACTGGTTAGGGTCAAAGTCGTGTTGGATGCGTTGAAAGTCAGCTGCGTCAATTTGGTCTAAAGTTTCAATAAAACCGTTAATGTATTTCTCAAAGAAGTTACTCATCCTTTTTGTTATCAAAGATAACTTCGAGCATTTGTTGAGGTGTTAAGGTACCAAAGTACTCCTTAAAGTTATCAATGTTACCCAATATTTTAGCAAAGATTTGGTAATCGTATTTTTGTCCTACCAACTGCTGGGTAACGGGGGTAATGTCGATAACACTCAAAAAGTCCCCATAGAACTCAATGTCGACTACTTTACCCTGATCTACCTGCACGTTACATTCAAAGATGCCCGCACCCTCAAACCGTTTCTTGTTTTTAAAGTTGTAGTCAGCTGTTTTACCAAAGTTTCAATCCCAGGATTGGAAGTGTTCCTGTGCACGTTTTTCCACCATCTTAATGCTGCTTTCATCCAAATGAATCGTTTCAGCATGTTCGGTTTCGGTAAAGAACTGCACCATCTTTTCCAAGAAGGTTGGGGTAGAGAGGTTGGGGAGGTATTCTTTTACGTTAACTACCCGTTTTTGGACACTTTCAATTCCCTTACTTACCATTTTTGTTTTATCCACATTGAGGTACTTCGCTAGCTTGGTAAAGTCGGTATCAAACAACAACGTACCGTGGACCAACACGCGGTCTTTGGAGAGGTATTCCGCTAAACCAGAGAACTTCTTGCCCTCAATTTCCAAGTCATTGCGGCCATGAAACTGTGCGGGTACACCTACACTGTTTAAGAACTTCACCACATTACGGGTTGTTTCTTCATAAGCGTTTTCCATTTCCTTTTTTGCTTTGGGCAAAATAATTGAGAAACAGATGTTACCCATGTCATGAAACACCGCCCCACCTCCAGAAAAACGGCGAAACAAATTAACCTTGTCCTTTTCCACTTCACTAAGGTTAACCTCAGCGTACGTATTTTGGTTGCGCCCCACCACAATCGTGTTGGCGTTTTGCCAGAAGTAAATGACTTTAATTTCCTCGCCCTTTTTAAATTCGGTGAGTAGCCATTCTTCTAACGCTGCGTTGAAGTAAGGAATGTTTTTAGGGGAAGTGAGAATGTAAGTTTTCATATTCAGTTGTTGATTAGTTAGACAGTTTATTTAAAGTGATCAAAAAGAGCTTTTTTGCACACATCAGCGATCATTTCGTTGATGGTAGGATGTGGTGAAATGGAGTTGGCAATGTCAAATAATGTCAAACCTGCATCCATAGCCAACGCTAGCTCGGCAATCATGTCACTAGCAGTAGCAGCAATAATACAGCAACCCAAGATCTTACCGGTTTGTGGGTCAAACATCATTTTGACAAAACCGTTGGTTTCATTGTCAGCGATTGCTTTTCCACAGTGCGCTAAAACAAGATTGGTTTTGACATAAGGAATGCCCTGTTTTTTCAACTCCATTTCGGTGTAACCCACCGATGCTACTTCCGGATTGGTGTAAATACAGGAAGGGCAAGTTAACTTTTGCGCTGGTTTGACCTGCTTCTTGTTTAAGATATGGTTCACCGCATACCGCCCTTGTTGGTAGGCAAAGTGGGCCAACATTAACTGGGCATTGGCATCGCCAACTATGTAGATGTTGGGAATTGAGGTTTGGAGATCTTGATTGAGCACAATCCGGTTGCGCTCATCACGTTGTAAGTTCAACCCATCAAGACATTCCGTGTTGGGAATGCGACCAATCGAGACCAAAATGCGATCACCAACCACACTGCCTTCCTGCCCGTTTTGCGAATAAAAGACTTCATTATTGTTAGCACGGGTTACCTGTGCGTTGGTAATAATCTTGACATTCTTGGTTTGCAAGAGTTTAGCGACTAAATCTGATACCTCAGTGTCAAAGATTTCCAAGATCCTATCCACCCCTTGTAAGATAGTCACTTCACTACCCAATGAAGCGTAGAGAAAGGCAAATTCAATACCAATCACTCCACCACCAACAACAACTAACTTGCGTGGTACCCCTTCCAAAGAGAGCGCTTGGGTCGAGTCAATTACAAAACCGTTTTGACGCGCTTCTGCAAAACCCGGAAGCGTTAAGTAACGGGGTCGTGAACCAGTTGCGACCACAATGCTTTTGGTGGTGTAAGTTTTACCCGCTACCTCCACGGTGTTGGGATCCAAAACCTTGGCTTCTCCCATTACCGTTTCAGCTTTAGCGGAAGCAATAATCGCTTTAACCCCACCAACTAACTTACTCACTACCTTACCTTTTTGCTCCAGCAATTGGTTTCAGTTAAGTGCTACTTGACCATTAATTGAAATACCATAGTCCTGCGCATGGCGGAGGTAATCGACAATCTTGGCCCGTTTTAACAGTGTTTTCGTTGGAATACAACCAACATTTAAACACACGCCACCAAAGTATTCCTTTTCGACAACGAGTGTTTTAAGTTTATGCTTACCCGCGTACTCAGCGGCCACATACCCAGCGGGACCAGCCCCTATAATAATGAGATCGTAATTCATCTAAGCTACTTCTAAATCAATTAATTCTTCGATTTGCTTGGCAATTTCCTTACCAAAGCGACCCACATCAGCGCCATCGACCCAGCGGTGGTCGGCAGCAATTGTTAACGGTAGGATGGTATGAACGGCTACCCCACCTTCAGCACGCACAACACGTTCTTCCATGTTACCGGTAGCGACAATACACATTTCCGGGTGCTTAATAATCGGGGTACCAAAAGCAGCGCCTAAAGAACCAAAGTTAGTTACCGAGATCGTTCCCTTACTTAAATCAGGAAGCTTAATTTGCTTACTGCGGGCACGGTTAGCAAGATCAACAATGTCCTTGGCAATGTCGACTACCGACTTCGTTTGTGCTTGCTTAATGTTAGGCACAATCAGACCATCGGGAGTGTCCACGGCAATACCTACGTTAACATCCTTGTTTAACACAATTAGGTTGCGTTCCTTGTCATAACGACCATTGAAAACAGGAAACTTCTTCAAAGCATTAACAATTGCTTTAACAAAGAAAGCAAAGAAGGAAATCTTCATGTTGTACTTGCTTAAAGCTAAGCCGTTAACACTCTCACGGTACTGCTTTAACTTAGTAGCGTTAACATAGAAGGTGAGAATCGTAGCGGGAATGTTTTCGTGTGATTTCACCATTGCTTCAGCAATTGCTTTACGCATTGTCGTGATCGCAATCGTTTCCTCACCACTAGGTGCTGCTGCAGATGCTGGAGCTGGAGTGGGAGTTGGAGCGGATGTAGGTTGTACTACAGGAGCTGGTGTTGGTTGGGGCGCACTTGGCTGCACACCAAAAATAGGAAACACACTGTTCGATACCTTAATTTCACCCACTACCGAGGCACCCGCCTCTTCCACTACTGGTTCGGTGGTTACAGGTGCTGGTGTTGGGGTAAATGTTGGGGTTGGGGCAGGAGCTGGTGCAGATACTGGTTGGGGCGCAGCAGGTGCAGCAGCACCAGCGCCATCATCAATAACGGCCATCACCTGACCAATGTGCACCACATCACCAACATTAGTGGTAATGGCGGTAATAACCCCAGCATAAGGGGATGGAAGTTCGGTGGTTACCTTATCGGTTTCCACTACAAAAAGGGCTTCATCGACCTTGATCGTATCGCCCACTTTTTTGAGGATTTCAGTTACTTTACCCTCATGTAAGCCCTCACCAACATCGGTGAACTTAAACTCATTTGCCATGATATACAAACTGTTTTAATTTTAGATTTAAAGCAAAAGTAATGCAAATAAAAAACCAGTCTTACGACTGGCTTTTTATCTAAAACGAAATTTAGAGAAATTTCGCCTTATTTTTGGCAGCATTTGCAACCGCCACAGTTGCATTTACTGCAGTTAGCTGTTTCGGTTTTGCAACCACAGCATTTCTTGTCTTCCATCTTAATATGAAATAAATGGTGTTCCCTAGCCTTCTCAACTTTTAGTTAAAAAAACTAGGTGCAATTATTCTAACAAACCTCAAACTTATTTCAAATGGCTTGATTTAGAAAAGTCGTTAGTTTACTTTAAAAGTTGGTTAACAGCTTCCAAAATGCGGGCATTTAAATTGAACTGGTAGTGTTCACCCCGTGCTAACGGTACCACAATGTCCCAACCGGTTACCCGTTGTGGGGCTGCCTTGAGATAGCTAAATAATTCTTCAGTTACCGATGCAATAATTTCCCCACTGGTGGTAAAGGTTTTAGCCGCTTCGGTAACTACGAGCAAGCGCCCGGTTTTCTTCACGGAGTTAAACACCGTTTCCTTGTCTCAAGGGGAAATGGTACGCAAGTCAATGAGTTCAATGCCCTTGTCCTTCAACTCACCCCCATAGACCATGTTAATTAGGTCAAACATGGTAGGACCGTAACTAACAATAGTAAGGTTGTTACCCTGGCTAATTAAGTTAGCTTGACCAATTGGCACAGTGTAATAATCTGCTGGAATTTCCTGACGGAAGGCACGGTACAGCTTCTTTGGTTCAAAGAAGACCACAGGGTCAGGTGATTCCACTGCTGCTAAGAATAAGCCCTTGGTGTCGTAAGGGTTGGACGGCATCACTGTTTTAAGTCCGGCAATTTGACCGTAAATCGCTTCCAAGGTCTCACTGTGGTGTTCAAGTGCTTTAATCCCACCACCCATTGGCATGCGGACAATAATCGGACAGGTATACACACCGCGAGAACGGTTGCGGATCCGTGCTGCGTGGGTAAAAATTTGGAACATTGCGGGGAAAGAAAAACCGGAGAACTGGATTTCCACGATTGGTTTAAGTCCACCAATGGCAGCGCCCACACCAATACCAGCCATAGCTGCTTCGGCAATCGGACAGTCCCAAACACGCTCCTCACCGTACTTCTTTTGTAAACCCTTAGTAGCACGGAACACACCACCTTCAAAGCCAGCATCCTGACCGTATAAAACAACGTTGGGGTCGCGCTCTAACGCTAGATCCATTGCGTTACCCAACGCTTCAATGTTATTTGCTTGAATTGTTTTTGACATAGTGAATTTATTAATGTGAAATTAGTCTTTAAAGTATTTTTTAGCTTCAGCCTTTTGGCGCGCTAGATCAGGAGTCAACTTTTCATAGTTGTAATCAAACACTTCATCCAAGGTAACTGGCGTTTTGCTAACCATTACCTCATATGCAGCTTGCACTTCTTGTTCAATCTTAGCTACCATTTCCTCTTCTTGTTGTGGGGTTAGAATACCACGGTCAAACAAGAAGTTACGTAACCGCTTCACAGGGTCACTCTTCATCGCTTCCGCTTCCTCTTCTTTAGTACGGTAAATGGAAGGGTCATCGGAAGTGGTGTGGGGACCTTGACGTCAAGAGAAGAACTCAATTAACACTGGTCCATTACCGCTTCTCGCATAGTTAGCTGCTTCGTGCATTGCTTCGTAACTCGCAATTAAGTCATTACCGTCAACGCGAATACGAGGAATGTTAACCGCAATAGCCTTGGTGGAAAGGTCACTTACAGCGGACTCTAATTTAGTTCTGGTGGAAATGGCAAACTGGTTGTTGTTAATACAAAAGACCGAGTTTCACTTGTGAATGGAAGCAATGTTCATCGCTTCATAAAACTCACCTTCAGCTGTACCACCATCCCCAATCATCGTAACGGCTACGTTAGGGAGTTTCTTGTAGTGCAACATGTAACCAAGTCCAGCAGCATGAGAGTACTGTGCACCAATGGTAATGTTAATGGGCAGCGTTTTGTATTTAGCTTCGATCTTACTACCGTTTTCGTTACCGTTTCAGTAAAGTAAAAGCTGTTCTGGTTTAACACCACGGTACAACATTAACGCACCGGAACGGAAGGTAGGGCAGAACCAGTCATTTTCATTTAAACCCATGCCCATACCAACTTGCAGTGCTTCCTCACCTAAGTTAGGCGCAAAGTTCAACATCTTACCAGCACGTTGTCAAACAAGCATCTTCTTGTCCATGATTCGGGAAAGATTCATGAGGTAAAAAGCGTGCTTTAACTGTTCGTTGCTAAGTGTAATCTTGTGATTAGGGTCCATTAACTTACCCTCATTGTCATACACTTGGTAAAGTGTAGTAGGGACTTTGTTTTTAATCAAAATTGCCATAATTATTTGTTAGTTTAATCTTATTTATTTTTAGGGGTATAGTAACTAAAACCAAGCGCTTGCAACACGGCTGCTAACACAAAGTTGAACGGCTTATTGTAGTGGGGAAGAAAGTAAACGTCTACTAAACCCAGTTCACTGATCAACATTTTCTTCTGTACTGCTAAAGCAATATAGAAGATAATTTCACTGTGGTTGGTGTTTCACGAAAGTAATTGGGCACCCAACAAACGGAGCGTCTTCTTGTCGTAAATTAACTTAAAGCGCACCTTGTCAAAGGTCCCCATAAATTCGGGACGGTCGTTGTCATCAACAATGCTCACTCCCACATCAAAACCGTTCATTTTGGCACGTTTTTCGGTTAAACCAGTAGCTGCTAGATTTAAACCAAATACGTGCAGCGCATTGGTACCAACAATGGACTCTAACTTTACAGCCTTGCTACCAATCATGTGCATAGCAGCGACTAAACCACTTTTCACAGCGTTGGTAGCTAAGTCAATGTTTTCGTATTGTTCACTAGCTGCATCATAAATGGCAGCAGCCCCACCAATAACATACACATTTTCATGATTTAAGGCTTGGAGGTATTCATTAACCTTAATCGAACCGTTACGGTTAAACTCAAACTGCCTGTCTTTAGGGACAAACTGGGTGTTTGGGCGGAACCCAATCGATTGGATGACAAGGTCAGCATCCACCCGACCCTTATCGGTTTCAACACCTTTAACGACATTTTTGTCCGCATCAACAATAAAGCCCTTAACGGCACTACCCATCATTAGGTTAATGCCCGCTTTCTTCATGGCTTTTTCCAACTCGTTGGTAAACTCTTCATCAAAGTTATTGCCAGCTGGTTTGTCCAACATGTCAATCACAGTCACCTGTTTACCACACTGTCAAGCCGCTTCTGCTAACTCTAAACCAATGTAACCCGAGCCCACAATGGCTACTGATTTAATTGACTTGTCATGACGGAAGCTGTCAATTAAGGTAAGGGCGTGTTGGTACAACTTACAACTAATTAAGTTCTTAATATTGCCACAGTACTTATCAGTGTGGTTGAATTGCAATTGGGTGTGGGTTACTTCGTTTTCCACATTCATACAAATCGGTCAAGCTCCAGAGGCTACTACCAATTGATCATAGTGGTCCACAGTTTCCTTACCAGTGGCCAAGTCCTTCACGATTACTTGTTTTTTGTCTAGATCAAGACCAACTACATCATGAGCCATAAAGACATTAGCACCCATAGCTTTTAGCTCTTCAGGGGTGGAGTAGAACAGGTCTTCGGTGTTTTTAACTACACCACTAACTGCCAAGGCAATTCCACAACCCAAAAAGGAGATGTTGGTATTGCGATCATAAGCGTTGACCTGAAAGTCTTTGCTCTTGCTTAAAAGGGTTCTGATAAAACTGGTGCCGGCGTGATTGACACCGATAACAATCACTTTCTTCATATCGTAATCAATGTCTTAAGATTAATATTTTCACGCCAAACTAAGTTTAGTTAAGTGCGGTATTTATTTAATAGTGGAGCAAGATCTTGGTTGCCACATTTGGTTGTAGCTTTGCTTTACCGTGCAGTTTTTGCAGTTCAATTAAAAAACAATAACCCACAGTTTGACTACCTAAACTCGCTATTAGTTTGTCAATCGCTGCAACCGTACCAGCAGTAGCTAGTAAGTCATCAACAATCACACAGCGCTTGCAGTTTTCTAGTGCATCAACCCGCATTTCCAAGATTGAGTTTTGGCGGTATTCCAAATCATAGGTTTCACGCGCTAACTCACCTGATAGCTTATGGGGTTTGCGCACTAACACTAGGGGTAGTTTGGTTTTGGAAGCCAAAGCACCACCAAAGATAAAACCACGCGCTTCGGGACATACGATGCCATCAGCTTTAACTTCCTGGATAAACTGTGCCATTTGTTCCAGTACAAACTCAAACAGTTCACTGTTTAAAAAAATTGGCGTAATGTCGTAAAAGAGGATTCCCGGAGTGGGAAAGTCATTAAATCGCTTAATAGCTCGATCAAGCGCTTGGAGTTTCTGTTTCACTGTTTATTATCTTAAAAACTGTTAAAAGCGTTTCTTAAAAGCTGTTAATTCCACTAATCAACTGTTCTTCAATTTGATCCACTTCCTTAAACATTTGCTTTTTGTGGCGTAAGGTAATGTGGATTTGGACATAGTTGTACGGACTTAACCAACCATTGATTAAACCAAAGCTAAACAGCGGTAAGCCATTGACAAACACGCTAAACAAGGCCACAATCGCAATTAAGTAGAAGATCACTAGGTTAATCGTGCCGTTAATACCACCAAAGTTAAAGGCAGCGTACACAATGAAGAGTAGGATTAAAACACTGCTACCAATAAGATCTAACCTTCATTGGCTGTTAACATTGTCGTTAAAGAGGTTTTTCACAAAGACCCGGTCCTTCAAGCTGCGTTTAAACCAGTAACAGTAAGTCCAAATGGTAGTTTGACCAATGCGGGCTTGGAAGAACAGCCAGAACAGGTACACAAAGATAATTAGTTGAATTGTGGACTGGTCAATCCTAAAGATAAAGTTAGCGAGGTACATTGCCCCAAAAAGTCCAGCGGCTAACAGAATTAAAGCAATTAGGTAGCTAATCCCATAAGCGACACCATAGCGCGGTAGCGAATACAAGAGATAGAGCAAGCTAATCACCCCCACACCTAACATAATGCCAGTACTCGATTCGATGTCAGCGGCTAAAAAGTTACCTAACAGTTGTGGTGCGCCCAGATAAAGGCCGAGCATCACCACCCCAAAGGCGAGAACTGTCAACCAAATAAACAGCAAGCTCCACTTTTTCTTACCAAAGAAGTCGTGCTGGTTCTTACTGATAAAGAAGTCCGTTTGGATGTCGTTGTTTTCCGTTAAGATCGAGCAGTTACTCGATGTTTTGGTCAACGCTTGCGCGTCCTTTTTATAGAGGAAGAAGCGTCATTCTAACCCGGAATGGGATGACACAAACAACCACACTAAGATTACTCCTAAACCATAGTTTAAGAAGTAAGAGGTAATCGCTGAAACTGCCATTAAGGTACCTAACTGTTGTACCTGGTAGTTGGAGAGGTAAATCACGACTAAGGCACTCAATAACCAGCAAATGTGGGTTTCTATCATCGTGAAGAAACTACGTTTTAAACACAGTTTTCACGATTCCACAATTGAGGCGTTGTTCTTAATGTTACGCAAAAACAGTTCCGCCAGAGTGATCAGGTTAATAATGTTAACGGCAACCACAAAGAAGATGCCGACAAACGAAAAGACATCAACGACACCACCAACGGCATTAAAGAAGGCGAGCGAAGAGATGGCACTAAGCGCTAAGGCTAAGGCTTTGTAAAGGCCAAGTAGCTTGTAACGAATACCTAACGCAATTGTAGCGAGCAGGATAATTACACCCAAGGCAATGAAGCTCGAAGCAAAGTTAGAAACATCGCGTGCTAAAGTGTTGGAAAGCGGTGCATTAACTAACTTGGTGTTAGCAGCTGCAATGGTAGCGGTTTCGGGAAAACCATTGCTCAACAGCACCTTTACCCTACTAGCTTCCAAGTAAGAAGCAGGAACGTATGAACCATCATTGTTAACAAAGTTAGCTACTTGGAACTTAATGTTACCACCGTGTTCTAAGCGTTGGTTATTGCTATCCAAAAAGCTTTGTAAAGTGGCGTTTACCTTAACCGGATCACCATTCTTAGCTACCGGATCTTCTGTTCTAATTTCTGCCAAGATGTAATTGGAGAATAAACCAGAAAAACCAGTAGCATCACCGCTGCGCAGTTTTCTAGTGTCCGCGCTGTTAGCTACAGTGACAACATCAGCAGCGTCCTTATTGTTATTGTCTTTGGTTGCAACTTGTGAGAAATGTGTCGGTGCTGCATAGATGTAATAGATATTTTTTAAGGTAATGTCACTAGCTTTGGTAATTGGTGAATTACCATTATTATTTTTTTGCAAAAACAAATTATCACCAGAGGAAAAGTTACCCTCAGCGTGCAAGAAGTCCCAGAGGTTCTTTTCACGTTCGTTAAAGGTGAGGTAATCAGTACCACCTTTAGGAACGTTAAAGATGTTGCGCACATACTGTACTGCACCTTCCTTATCCTTCCATAAAACTAAAGTTTTATTTCCGTTTTGTGCACTACCGTTAGCCTTGTCTTTAAATCATTTACCGCTACCTTCAACACTCTTTTGACCATTACCAGTATTATAGTCAGAATCAGTTAAACTCTTACTCTTAATAAAGTCCAAAAAGTCGTTGTGTAAAGATTCTTTGGTAATAACATTTTTGCTGAGCTTGTACTCAAACGTTAAGGCACTGTTGGTGTTTGTGGTTTGGGGAACAACTGCTGTAAGCGAATTTCTAATAAATTCCTTATCAAACGAGTCAAAAAGAACAATTCCGTCAGTGGTTTCTAACGAAAGTTTGTAGTTGTGTTCAATTGCACTTAAAGCGTTGCGCTTGACCTGTGCATCTATTTGCCGCTTTCGTGCTCGGGTACGCGCCTCTTCACGACGTTGCTCAAAGGTAACAGAAATAGAGGGTAAACCATCATTAACTAGTCAAGAACTGTCAAAAACTCCTTTATCTGAAAGTTCTTTTGCACTTGTATCGTGTGAGTTTTTCGTGATGTTAACAGACGAAAAACCCTGGGCGTAGAGGCTGTTGGCGTAACTCTTTTCAATATTGTTGAGAAAACCGTTCACATCGGTAATCATTTCAGTATTGTTACCGTTGCCATTGCCGTCCTTTTTCTGGGTAGGATCTAACTCCTTGTTAGCGTCCTTGGACTTATGGTTTAAAAAATAAACAGTGGTGGTAGTGGAACCGTTGAACACTACCCCTAAGCGACTGTCGTTTAGAAGCTTATAACTACCAAAAATAATTCCAAACAGGCCCAAAAAAGCTAAGACCAGGATAATTCCAATCTTTAAGAGCCAGTCAAAGGAAACCCGCTTTTTCAGTCCCACTGGATGTTTTACAATTGACTTAAATTATATAAATTTAGCAAAATGGCCACTTTAGTTGAAATTGAACGCGATTTTTTACAAAAAACAGCCCAAAAATTTGCACCTGAAGTGGTTGCGTTGATTACTAAAGCACTCGATTACTCCAAAAAGTGACACGGTGAGCAAAAAAGGCTGAGTGGTGAACCGTTTTTTATCCATCCCTTGAGAACAGCACTGCGCTTAGTGGAGTGAAACATGGACAGCAACACCGTGTGTGCTGGTCTGTTACACGACATTATTGAAGATACCCAGGTCACTGAAGCTGATTTAACTGCCATTTTTGGTAAAGAAATTACTGATTTAGTGGTAAAAGTTACCAAAATTACCAGTGAATCCAAAAAACAACGCCAGCTAAACCGAAAGAAAGAAGATTTAAACCTCAAAAGTTTGGTTAACATTGCGATGAGCTCGCAGCAAGAGGTCAATGCTTTGGTGTTAAAGTTGGCCGACCGACTCGACAACATCTCCTCAATTGAATTTTTAGCAGTCGAAAAACAAAAGATTATTGCTAAAGAAACACTCGAACTGTATGCCAAGATAGCCGGTCGAATTGGAATGTATCCCGTTAAAACTCAACTAGCCGATCTTTCCTTTAAGGTATTAGATCCAAAAAACTTTAACAATACGCTGTCCAAGATTAACCAGCAAAAGGTCTTTTATGACAACGAATGGGACAACTTTAAAAAACAGTTGGAAGAGATGCTGGAACAAAACCAGATTGAATACCGCTTAGAAAGTCGAATTAAAGGCATTTATTCTACTTATCAAAAATTAACCTTTCACGAGCAGAACATTGCCAAAATCCACGATCTGTTCGCCATTCGTTTAATTGTGAAGTCAGAGTTGGATTGTTACCACTTACTTGGTTTAATCCACCTCAACTTTACCGTGTTAATGAAACACTTTAAGGATTACATAGCATCCCCCAAACAAAACTTTTACCAATCAATCCACACGACTGTGCGCTTGAAGGGTTTAAATGTGGAAATCCAGATTAGAACCCAACGAATGGATCATGTGAGCAAGTACGGTTTTGCTAGCCACTGGATTTACAAAGAGAAAAAGGAAGGGTTATTGGCAAGTGCTTTACAAGTAAATTACTTAAACAGTAAACAAATGCATTCGCGCGATTTTTTTAAACGAATCTTTGGTACTGATATTATTAAGGTTAATGTAAGTTCCGATAACGAACCTAACATAGTCAAAAAACTCAATGTTGAAAGTAACAGCAAGTTGCTCGATATTGCTTATGAGTTGTATCCCAAACAGTTCAATAAATTAGAAAAAATCAAACTCGATGGGGTTGAGGTTATGTCGTTTGACGTAACTGCCGAAAACGAAATGGTAATTGAGTTTTGTTTTGGTAAAACCAATAACCTCAAACGGCGTTGGTTACGTTATATGAATAACCCCGTATTTCGCGAACGGGTTAAAAAGGATTTAAACAAGTTAAAGAAAGCGGTTAAGTACAGTGAACTACCTCTATACGAAAAGGCCTTGGAAGAGTTGCACTTAAAGCTAGCTGATGAAACCCAGATTAAACAACGCTTAAATGCTTTAGGGATCAAAAAACTGACAGAGTTCTTGGAATTAATCGAATACCCCCACTTCCCTAAAAATGAGCATTTGTACTTTTTAGCTAGTAACAATCAGAAGTGAAGGGAATTGATTAAACCCATTAAGTTCGCTTTATCACAAGCTGTGTTCCAAAACTCTTACTTTGAACAAATTGAGGGAATTTACATCACCAAAATTGTGATTGAAACCTGTTGCACAAAGATTCCTGACATGCCAGAACAAGTTATTGGCATCTTAATGAAAAACATTCTCAGGGTACACCTTCATGATTGTAGGGAATTAGCTAATCAAAAGCAACCCAAAATTATTCCACTTTATTGAAATGCACACCAATTAAAGATGCGACCACGTAAATTCCGCTGTCAAATTAACATTCGCGGTGTATGGAGTGAAACAACGGTCAATAAAATTGTTCAAACCATTATTGAAGGTGATAGCTATTTAGAACGAATTATTCCCAAGATCGATAAACAAAAAGATGAGTTTGAGCTCAACATTACTATGTTTATCGACAACTATCACCAACTAATCACAATTATGGAGCAGATTACTACTAAGAATATTAGCTACGTTTGAAAGTACCTTTAACTTTCAAATACTTCGTCATAAACATCTTGGTTCTTTTGTAAAAGTAACACTAAGTGTTTCTTCAATAGCCTTTGTGGGTTAAAGCGCGATACTCGTACCCCATAAACGCGTTTGGTGAAAATAAACAATAAGGCACCAGAAACAACAAATGTACCAATAATAGATACCCCGACATAAAAGACAATTTGTGAAATATTAGTTACGTATTCCTTGAACTGATCATTTTCGTTAGGATTTGGAAAACGTTTTAAGATTTCGTCCACGTATTGATTAATCTTTTCAATTGTGTTCTTAACTTCAGTAAAAGTAGATCCTTTTGAGAAGTTGTTAACCTTGCTTAATGAATCTTCGAGTTGGTTAAAACTGCTTTCTAAAGCACTGTCATCCAGTAATTTTGTCAATTCTTTTCCTTCTTTAATTGAACTTTCAACATCACCTAACGTTTTTACAAGTTCGTTACTAGAAAGTGACTTTGAAACTTTTTTAATTTCCTCGTTATATTGTTTAACCTTGTTATAACCTTCCTTGTAATAGTTATTAATAGGTTCACGGTTTTCAAAGGCAAAGTAACCCGCTGCTGTAATGCCACCAACAAGTAGAATACCAATCAAAACAATTAGGAAAGTGGAAAGCTTTTTTAAAAACTTAAACATTATTGATCAAGCTCCTCTAAAACAGCATCTTCCAACTCTGGATAACGATCTAAGATGTCATTAACATGATCAGCAATTTGATCAGTACTGGTTAAACGCGAAAACGTTCTAAGTCCTAACTTTTTCGAACCTAAGAAGGTCATTACAGTAGAGACTATTAGCACTGCTAAAACGCCACCAGAAACACTAACTAAGGTAATTGCGCCTATGGTGTAATATTCATTAAACTGTTTTTCTGTTGGAATTTTGTTTTTAATTTCTTTGTACTGTTTAATGATGTCATTTGCTTGTGTAGAGAACTCTTGAATATCTTTCAAAGCACTATTGGTTTTACCGTTAGTATCTCTGGTACTTACACTAATTGTTGAACCAGAAATCTTAGTAATTGGAATAGTCGAAGAAGATGTGCCATTTTGACCAGTTTTACCATTATTAGCGGTTTTTAAAGTATTTGCATACTTTTCTATTTGACTCTTAGTAGCTTGTAGACTCTTCTTAGCTTCTTGTAACTCCTTTAGTTTTTCCGGACTCACAAGATAGCCGTTTAAGCCTTTAGCTTGGTTTATTTGCTTTTCGTACTCCTCTAACTTTTTAGTTGATTCTTCAACTTTCTTCTTAACCTCTTCTATTTGTTTATTAATGGATTCAACACTAAACTTACTGGAACTTCCATTATAAGTATCTATAAACGCTTGAGAAGAGTCAAAGGAACTGTCGATCGATTTGCGGGTATCAATGTAAAGTTTAGCTGCGTTGTCTTTAAAGTAAAGTGCAGCACCGCCAAGACCTAACAAAACCAAAAAGACAAGCATCCCGAAGAACAGAATGATTGTTTTGGCAAAGCGATTAATAAAAAGGAGCATTTAAAAGCTTAAGTTAGGCTTAAGGTTAATATATTTAACCAAACGGACAACCCGCTAAAGGTTAAGTTCGAATCTCTTGGAAAACATTTTCCCGATCCAATTTTTCTTTCAATTCCGCTACAACGGTTTCATCTTTAGAAACATTAGTAATATTGAGAATTTCGGCGTTATCACGTAATACACCGATTAAATCAGTAACTGATTTGCTCATATCAGTGACACTACCAATAACTGGTTGGAACTTATCGGTACTCTTTAATGCCACTTGTTCTTGTGGACTTTCAAACTTCACGGTGTACTTTAAACCCGGACGGAGGTTTTTCACTCCATATTCAAAGACAACCCCAATAAGTTCAGCGCGTTGTACAATTTCCTTTTGGAACTCACTATCACGGGTAGCGCGTTCCGCTTCGTAAGCACTGGCACCCGGATAGTAATTTTCGTTCACCCGGTACATCGTGTTGCCGCCATGAGTGTAAGTTTCCTCTAAGTGGAGATTAATGTCTTTTAGAGCAGCGTAAACGGTGTCGTTGTCTTTATTGACATTTTTTCATTAGTGTTTTTCACCAAATAACGGTCAATGTAACCACCAGCTCAGTAACCTTGCATTTGTCGTTCAAAACGACGACGAATGTAAATGTCACTAACAGCACGCTTTAACTTAGATGCATCAATATTGTCTTGGTTATCTTTAAGTTCTAAACTAGTTAAATCAATGTAATTAAACGGCTTGGAAGCAAAAAGGTCATAGATAATATTAGTGTGATCGCCTAAAACTAGTGGATTAAAACCAAAGTTATTGTCTTGGTTAGTAGAGTAAAACTCCAACTCTAACACGTTTTTAAAAATTTGCTTAGCAACATTAATCGAGTGGTAGTCACCATAAATTGACACCTTTTTAACGCTTTCTGGTAATTCCAAATCCTTTAAGGTAGAACCATCACCAGTTAAACCATTAATGATGACGTAATCAGGATTACGTTGTTTTAAATTGAGGTTAATGCCGTCAGTATTACCTTGCTTAATCCTTGGAGCATCAAAGGCAATCGTTTTTATTTGCTTAAAGTGATGGTTTGGGTAAACGTTGTATAACGGTGAACCGTTAGCAACATAGTAATTCCACTCTTTAAACAAGTACTCGCTTTGTCCATTGGAGCCAGCATAGTAAGCCTTATCCACGTCATGGAACCAACCAGGGAACTTAGGGACACCTTGATCGACAAAGATATCATCGTAAGCCCTATTGTTAGCAGTTAAAGTGCCGTTGGTAGTTTGCAAAATTGGCATTACTACCCGGTTGTTAAAGTATTCGGTTGATTGGTAATATTTGAGCGTTTTCCCGTCCGCTGCTACTTGGTCGTAGTAACCCGCTTTAAAGTTTGGCGTGATAAAGGTAGAACTGGTTTTAATCAAACCGCTCACCTCTACCTTAGGCACTGGTCGAAGTAAGTCTTTTGGTGCATCAGTAATTGCTTCTGATAAGTTATTGGCAAAATTACGGAAATCCTCACGGAAGTTGGAACCATCACCAAGATCGATTTCACTGCGGTATCCCCCATCGGTTAACGAAATCGAATTAAGTTTAGACTTGGCGTTTTGGTTTACCTTGGAATAAAGCTGGAAACTCAGGTTACCACTGCGCCCCGAAGCAATGAGTGTTTCACCACTTTGTAGCCTTAATACCTCATTAACGATTAGAACTGCGGAAAAAGCATTTATTGCAGCACCAAAGAGACCAAGCGCTCAGAAACCACCCTTTTTGAGGCGCTTTAGCGTCTTTTTGTCCTTGATTTTGAAATTTAATTTCATCTTTTTTAATAGTTTAAAATTGCAGAACTAAAACAGTTCTTACTCAACTAAATACTTGAACTACCTCAACGACAACGGGAAATTTAATTGAACACCTATTAAAGATATAGTGTTCTTCTCTATTTTAGATTTAGTAATCCCTAAAGGAGAAAATTTACGCCAAATGTGTACCAAAAAAAGAGAAATTTTTGGCGATTACGTTATTCATTTCTTTCCAAAATAGAGGTTAAGCGATAATAAAAGCACCCGCTAAGCAGGTGCTAACCTTCTTTTGGAAATGTTTAAACTGGAGCGAGTAATCGGAATCGAACCGACACGATCAGCTTGGAAGGCTGATGTTCTACCACTAAACTATACTCGCAGAACTTGGTGCCCAAAGCCGGACTTGAACCGGCACGCTATTGCTAGCGTGGGATCTTAAATCCCATGCGTCTACCATTCCGCCACTTGGGCTGGTGACTCACCCGAGGATCGAACTCGGGACCCTTTGATTAAAAGTCAAATGCTCTACCGCTGAGCTAGTGAGTCAATAAACAGGGTTGCCCCTGTCATTTTATGAATGCTTTTTTACTGGCTGGGATGGATGGGATCGAACCATCGCATGATAGAGTCAAAGTCTATTGCCTTACCGCTTGGCTACATCCCAATAAATGGTGGACAGGGAGAGATTCGAACTCCCGAAACCATGAAGGTGTCTGATCTACAGTCAGAAGCGTTTAGCCACTTCGCTACCTGTCCAATAACCAGCAAAAGATATTTTACAAAAATTATCCGTTAATGATCTTAACAATTTTGACCCGGTAGGGCACTTCTATGCCAACTACGTCACATTCATCATCCACTAAACGACCATAAATGGCGTGTGCCAAAGGGGATTCATTGGAGATGCGGTGTTCTTCGGGATTAGCTTCTAAAGCTCCCACAATGGTGTACTTTTCGTGTGTTTTCGAACTGTAATCGTAAATTTCGACCGTACTACCCAGGGTAACCTTATTAGCCTTTTTTGTTTGCGACTCATTAATTAACTTAACGTTGGCTAAAATGTCTTGGATTTCAGCAATGCGGGTTTCAATTTCACCCTGCTGTGCTTTTGCTGCATCGTAATCAGCGTTTTCACTCAAGTCACCCTGGTCACGGGCTTCTTGCAATAACTTAATAATAGCGGGACGTTTTACTTGAATGAGGTGTTCCAGCTCCGCTTCTAATTGTTTTAGCCCCTCTTCGGTAAGGTAATTTTTGTTAAGTTCCATTAATTAGAAATTGCGTTTAAAGTTAAATTAAATCTACTTTTTTTCCCAATATTTGCCAAAAAAATGCAAAATTTTATTTGCAATTTGACCTAACAGCACTAGTTAAAGCTACTGCTTGTTGGATGACAGATGACAACGAAAGACAATTCTGGCATTAAAAGTAAAAGACGGATTCGTTTTTTAGCTTTAGGAATTATTATTTTGATTCTTGTAATTGCTGTAATCACCATTTTATTTGTCACGGGTGTAATTACCCGCGCTAACCAATAAAACGTAGTTGATGGCCAATAAAGATCAAAATCTAACGCTTTGATACGACCAACTACTGAGCAAAGCACAGTTAGTGAGCTATGGCGATGTGAAGGGCACTAACTGTTTTTTACCCAACAGTTGGAACCTCTGACTACAAATTCAAAGACTCTATAATAACGCTACTGCTCTAATTAAATTAAAGGACAAGGTTATTTTAAAGCAGTTTATCCCGATTGAACCACTGCCATACACAGTGGAGCAAGTTCAACTACCGACCCTTTCCTTTTACAGTGAGTACCAAAAGGAAAAGCGTCATGTGGAGGGCTTTAATCCTGAGCTTTTTTTAATAGAGCAAATAGGTACTAAAAAACTCCATGATCCGCTAGTACTACGTCCTACTAGTGAAATTGCTTTTTGTAACTTATGGAAAAAACAGAGCTTTTCTTACCAAAACCTTCCCGTTATCTATAACCAGTGAACCTGTGTTTTTCGCGCTGAAAAAAACACCCGCCCTTTCTTGCGCAACAGTGAGTTTTACTGACAAGAAACCCACGGTTTGTTTAGTGATGGAGTTAATTCTGAAAGCGCAGCAATTGCTTTTTGAAAACTGTACCAGGACATTATTGTCAACCAGCTCTGTATCCCCGCTTTTGTGGGGTTAAAGAGTCCCAATGAGCGCTTTGCCGGAGCACAAAATACCTGAACGGTGGAAAGCATTATGCCCGATGGGCAGGCATTGCAGTGTGCCACGAGCCATGATTTAGGGCAAACCTTTACCAAACCGTTTGGGCTAACCTTTCAAAACCAAGCCAACCAACAGGCCATTCCCTACAGCTTTAGTTGTGGAATTTCTACAAGAATCCTCGGTGCTTTACTGTTAACCCACAGTGATGACTTTGGACTGATACTACCATGAAAAGTAGCACCAATTCAAGTCAAACTGTACTTATTTGACAAAAAAGGTGATACAAAGACAGTAGAGTTAGCACAGAAAGTGCAAACGTTGTTGGAACAATTAGCAATCCGTTTTCAATTTATTAAGGTCGAAAACCAGCTCGGTAAACAGTTAGGACAGGGTGAAGTCAACGGCATTCCCTTTCAACTAATAGTTGACAATCCCCAAACAGTCAACATCTTTAACCGGTTAACCAGGGTCAAAACAGCTTACAGTTTCGAACAGTTGGCAAGCCGATTTGTGGAGCTCGTGCAACAGTACCATCAAGCAATGTATGACAAAGCCAAAGCAGTGGTACAGCAAAAGGTGGTGCAAGCCACTACTTTAAAGCAAATTGAACAAGCCTTTAATGACAAAAAGGCTGTTTTGTGTGCGGTTCGTTTAACCGATACATTAGAACAGCAATTAAAGGAACGGTACCAGGTCACGGTGCGCTGTTGCTTAGAGCAGCTTCAAAAGCCCCAAATTTGTCCGTTTAGCGGTGAGTCAGCACAGGACTATGTCCTGATTGCGCGTGCCTATTAATAACTTTTTACGAAAACTTTTTGCTTAAAAGCAAACCCTAAATGTAAAGGCTAAAAAGGTGGTTTTTATTTTCCACTTCTGGTCGTTTTTTGTCAATAAAATCAGTTAAACCTTTAAATTTTTTAAGGTTTTTTTGATCTATTTATGAGTTTTAAAGTTAAAAACCAGAGTAAGCACCTATACTCTTTAATGACGAAATTTAAACGTTCGCAACTGATCTTGAAGCACCAAAGCAATAACTTTGCCTCAGAGTTGTGGAACGAAGAGGACATTATCCGCTCCAAGCAGTTTATCGAACTTATAGAAGACACGCTGCTCCACCTCAAGAAGGATACGGTCGACTTTATTTACGACATCTTCATTTATGGGAAGAAGCCGTGTGATATTAGTTACTCCAATTCGACCTATTACAAAAAGTTAAACAAGGCTGCAAATAGTTTTTTTGACCATTTTGTCTGGGAGGCTCCAATTTATAAGACAAAGGAGCTTAAAAATGACAATTCTCACAGTTAGACAAATTCAAAATAATTACTATACGGAATACTACATGGGTCCGGTTAGAAACTTTGCTGACAAAAAGGAAGTTTACTTTGATGCTAAGGTAAACAATATTGAAAGCAAAGTCAACAAGGATAAAGCCTTGTTACACATAACCCTGGCGTTAAAGTACGACAGTAACTTTCCCAGTAATATGTTCCAAGCTCAGTTTAAACTGGGTAATTGAAGTAGTGAAAAGATTCCGCTGCAAAAAGCACCGGATAAAAATCCGATCTGGAAAATAAGATTCACTATTTTTATGCCACTTTGGAAGTGCCACGAACTGCCCTTATTAAACGGGAAATTAACCGCATTAGTGAGTATATTCGTGAAGAATTAACAATTGCTTTTCGCTTAAATGACAGTAGTGATCGCTACCATTGGAGTAACTATAACCTGTTCGATACAGTGGCAGCTGATATGTACCAAACGGTCATTAAAGAAACCGTTACCTTTGGTAACATGATTAGACTAAACCAACTCGAGGGCGAGAAGGAAGGTAACATCCAACAAAGTGAACTTAAATATGGTTGAACGATGTTGGACTACCGCAATATGGGGCCGCTGGAAGAGGTTAATAATTTAATTAATATTAAGTTTAACCAACCAGTAAAAGTGGTAAGTGTTGGTGTGACCGTAAGCTATACAGCGCCTGATGGTAAACGCCAAGAGCTCAAAGATCAAGCTGAATACCAGCACACCCTCAACCCCAATGAAGAGTTCAAACTGAACTTTCCCAGACGGCTAAGCTTTAACAGGGTAACTAAAAAGTTGGACTTTGACCCCAATGGTAGTGCCGTGTTCCTACCACAGGGTGGCTTTGGTAGCTATGAGATCAAACTGGAGGCTAATGTCGGCAACCAGTTCTACACAATTGTGGCTACTAACAGCTTTGAGTACGCCCATCCGTTTGACGATCCCAAGACCAATGACTTCTTTCTGGTGCAGTACGCTCCAGTCTACAGTCTGTTTAACTTTAGCGATTTAATTCAGTAATTGACCATGATCTTCTCAATTAGTAAACGCAAGTTAATTTGTGGCTTTTCCCTAGTAGCCCTTACAATCGCTGGGATTGTTGGTGGGGTTTACTTAGTCACCAAAAACAATCAACAAACCACACCACAAACAAATCACTTTAATGTAGCTGAACCAGTCAACAAGGTCCCTAATTGGCGCAAACTAGGTCCAGAAACACAACGCGAACTGCGCGATCGTCTGTATCCCTTAGATGACACAGGTTACTTTATTTACAAGTACGGTGCCATTAGTCGCTACCTCCAAAGCCAAAAGGAACTGGATGAACTAGTCGATTACCGCACCGTGTTACCCTCCACCCAAAAGCACTTTAAGTATGATTCCTTTAACCAAAGCGTGTTGGAAAGTAAGCTGCGTAAGTGGTTAATGAAAGCCATTAAACAACACCCGTACTTTCAGCACTTTGAGTTTGATCCGGTGCTTAAAGCACAGTACAATATCAACATTCCTGCTCAAAAAATTACGGTGAATGCAGTTTGGTTTTACAAAAAGGATAATGATCTCACCACGGGCAAGCCAATCCGCTATTGGGATCAGTTTGAAATTAAACTAAAGCAGTAGTAATAAATCTTTATAATTCTTATTGGGTTTACCGATGCAAGAGCGTGACATTCTTTTAAAAATTAAAGAGATCGCTAAGGCTAAAAACTTCAAAACTGAGTTAGATGAAAGTGTGTTGCAAAAACCCTTTCGCGAACTGAAAATTGACTCACTTGATATGTTTAGCGTGATTGTCAGCTTAGAAAAGGAGTTTGACATTATGTTTGAAGATGAAAAACTGATGCAGCTCAATAATCTCGCTGAGTTAATTGCTGAGGTAAAACACCTAATAAGTCAAAAAGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCATTAAAGATTTGGCCTAGTGTTGAATGACACTGGGCTTTTTTATTGCTTTGATTCAATCAATTCAAAGAAGTTGGCGTTTTCCTGATCACGGAAGGTGGTTTTCACGCGACGAATTTGCTTTACGTCAATGTTCCAGTGCTTACATTTTAATCATGGTGCAAAATCAGTTTTGTGTGTTTCTCACTGACTTCAGTTGTTTAAAACCACGGTAACTTGCTCGTTGTTGTTAGTGTTAGTAAAGGTGATGAAAACAGGTTCAAAGCCTTGTTTGTTAACCTTTAGTTTGGGTGGTACAGACTGCTTAATGTCGAGCACACCGGTTTGATCATCCACCGTTACTTAGGGCAACTCTACCACTGTTGCTGGCTTTTTGAGATAACCAAAACTCAAATAGCCCAAACTAGCAAAGGCTGAATTTTGTCAATCAAACCCAACAGTTGTTAAGATATTGCGCATGTATTCCACATTACCGTTCACTGAAAGGTGGGAAAATTTATCGGCAAAGTAAAACGACTTCTTTAAAAAATCAGTGTTAAACAGTTTGTTTTCATTAATATCAATCCCACTCACTTTAGCTAACTGTAAGAACAAACTCTTTTGGTCTAGTAAATGAACAAAGCTGCTGTAATTACTATCTTTAGGAATACCTTCGAGAAAATCGTTAAAGGTTTTTCAGTTCTTTTCAGAGCTTCGCAGTAAGCTAGATGGAGCAATGTTGGCTAAAACAACATGGGCTTGAGGACTTTGCGTATGTAACAGATCCAGTATTTGGGTAAGATTATGTTTGTATTGCTCGACTGTGTAAACCCCTTGCGCTAACTGGTTAATGTCATTAGTACCCAAATTAAGTACATAAACATCAATGTTGTTGTACTGCTTAAAGCGATGATTTTGCATGTACAACTCTAAGGAGGTGTTACTACCATTAATGGCACTGTTGATCACTACATCACGGTAACGACCAAAGTCATTTTCAAGCTTTGTTCCAACAGTTGTGGGGCACTACTGTATCCGTCAGTACCAACACCATGAGTCATCGAATCACCAATAAACAACCAGTTGGCTTGGGTTTTATCTTTTAAGAAATCAAAGATAGTTGGTGTGTTGTCTTTTTCTTTATTAATCCAACCGTTAATTACCTGGTATGAACCTTGCCCGTCTGTCCTTGTAATTTGAAGTAGGTAGTTGAGTGGCTCATTTTCTTTTATATCAAGTGCTTTATGGATATATTTCCAGTAAAGAGATATGTCAATTTGTTTGTTAGTAATGGTACCGTTTCAACGCAAATAGCTGGTAGGACGTTTCTTAATATCTCTAGCGTTAAAGTCACTCGCAAACGAAAGCTGGTACTGCAGCCCATTAGGATTAACCTCTGTACTTTCAATTTCCAACTCTTTGGTTTTAGTCTTTCAAGTAACAGTAAAGGGATTGGAAGTAGTTACCAAATTAGCTTTAGCATTATTGAAAAAGTCCGTTTCTAAGTTGTAGTTGGGATCATCAAAATCAATCCTGTCATTAATGACATTGGTGTCATGGTAATGGCCAAAGCTGTAGTAATTTTTTACATCAAACTGGGTTTCTTCATTAATTGGTATAGCTTCTAGTGATGGTTCTGTAACTTCCACTGGCAATGGTTCAGCGCCAGTAGCTTTTAACACTTGTGCTAACTGTTTACCAATCGCAAATTCTCCTTGCAAACTTAAGCTAACTTTGTCTTCACTCAAATAATTGCCGCTAAGTCAATCACTAGGTACAAAGTCACCTTCAAAGTGATCAACTACCTTAATTTTGGACGCAAAATTACGGTTTTGATCAGTTAATTGCTTAACGGTGGCTAAAGCGACAAAGTTAAGGACACTTAACTTCTCGTTAAAAGCTGAATCATTTGTTTTTCAGTGCAATTGCAAAATGAAAAAGTTGTCCTTTTCAGCCTTTTCACCAACTTGCTTTTTAATGAGTGCGGTTAGATTATTGACAAACTCCTCGTAATAATCAACCCGCTCTGCATCCGCTTGGGAAGTGAGGTAAACTAGCGTACGTGGTTTGTAAATTGAAAGTACCCGTTCTTGAAAGCTCTCATTAATATCTTTCATAGTCTGCCCGGCAAAACCAAAGTTTGCTATCTTACCTTCAAACCGAGCACTAGCAAACACAAACTGATCGCTAAAATTAATGTCGCTGATAGTTTGTTTATCACTGTTTCATTCGCATAAAACATCAACCCGATCAACTTGTCCAGCTGGTCAGTTCGGGTTGGATCAAAAGCGCCAGTACTGGTCAAAGTAAGCGACAAAGTTACGCGCACCGTGTAGTCGAGCAAAGTCGCTAACTAATTCCTTACCACCAGTTCAAGCAAAGCTTAAGGATGGGAGTTGGTACCTAATTTTGTTGTCATGTTCTTCACCATCCCTAGCATAAAAGTCCAATACCTTGCTTTGGAATTCACTACCCCAACTCGCTATTTCTGTTTTGCTAATGTTAGTTGGTAAATTTAAGGGTTCAATCTTTTGTTGATGGGTGGAGCTCGGTAGTTGGATGTTGATATTTTTCACAAAGAGTGGTGAAACATCACTCTGAGTGCCCATTAAAAAGAGGGTAAAGAAGGCACCAACACTTACACCACAACCAAAAAAGGCTAGAGAATTGAGTCAGTATCTGTACTTTGTGCGTTTTTTCATATTGGTTTATTCCGGTTCATTAATGAGTTATTTTTATTGAATAACAGCAACTGCAACTTTACCTGTTATTTAGCTATAACTAATTTTAAAACTTTGTAATTTTGAACCACATTTTTTTGAAAGAATTGGCAATCGGGATAAATTGCTATTTTGAAAAATTCAAAATTATTAAAATTAATCCCAAATGTTTTTTGGGTTTTCAAAAACTCTCTAATAACAAATAAACAATGGTTATTAAGAAGGGTTTTTTCGCGTTATCAAGTTGCACATTAGGTCTAGGTCTTATTTTAACTGCATGTGGTGCTAGGGGTAAATTTGACCAAGTTGACGATGGTGTTATTAAGCTAGCTACTTCAATCCAAAATAAGGACAGTATCGCTGCTTTAAACACCATTTACAAGAAGTATAAGGAACGCCATCCAGGGAGTTATCCAGTTCAAAATTTTCAGGTTCCCGGTGGTTACAGTGGTTTACGGAACGACATTAGAACGCGTTTATCAGCTAAAGATGGCAACAACTTTTACAACATAGTACTCAACTATCCTGATGTTGTCTCCTCATTGGCTACAAGTGACATGCAACTAATATTGGATAACGTTGACACCAAGCTCCTTTCGAAAAATTTCTTGAGCTTTAACGAGCGAATTGGTGGTGTACGACAAAAGGGGATTTATGCAATTCCCATTTCCCTTTCCACTGAATTAATGGTGCTCAACGGACCGGTATTGCACTATATTTTGAACAGTGCTAAGAAGAAGGAAAACGGTGCTCAAATGATTAAAAGATCAGGTAGTTTTTCCACTGTTCAAAAAGGTACCATGGCAATCGACATGAATGATCAAAAAACCAAAGATCTTTGACAAAAAATCGAAAACGCTGCTAAGGCTAATGGTAAGACAACTAGCACTCAAACAAGTCCACAACCAAAAAACGCTGTTAGCTCACTCCAGCTAAAACAGGCAGCTGAAGGTACTAGCACTGATAATTCTCAGGATGCAGAAAATAGCGATAACGAAATCAAAAAGACTTGGGGTGAATACAAGGAAGAGGGTAACCACACACTGAAGGGTTACACCTTTAAGGCTAGTGTCTTTGAAAACTGAAATGAACTATTAGACTTTTCCACGCGGGTAGCCAATTCGTTCCCAGACAAAATTAAAAATCAAGCATCTAAGAAAGCTACCGAACTGCAAGGTGTCTTTGGGGTTGACAGCGTCTCTGGTGCTTTATTTTCCGCTACCTTTGCTGCTGGTGGTGGAGACTATGATAAGTTCTTCTTTAATGTAAAGAATGGTCGGGGTAATTTTAGAAATTTACTCGAAAAGGGTAGTTCTTACAATAATCTGCAAAAGGTCTTTAACGATTACAAGCAGTTAATTAGCTCTAACGGTTTGTACATTAATAAAGGTGGTGCTTATTCCAGTAACTTCTTAAAGTTTCACCAGTTAGCTTTTAGTGTGGGTTCCAGTTCTGGTTACAATTTTGCTTTTGCTGGTGAAAGCGCTAAACGCTTGGAATTTGGCCAGAAAGCAATCGAGTACCCACGTGACACTTACGAAATTAAGGCACCTACAAATAGCCAAAATGGTAATGGTACCCTTCTAGGTAGTTTTACTAAAAGTAAAAGTAATGGCAAAGAACAAAGTGGGCAAGATGAGGATAACCAGACCAGTGAAACGATTGAGCTTTACAAAAGCAGCGTTCCTAGTGGTAAAGAGGCGGGTAAAAATGCGCTTGCTATTACCAATCAACAACTAATTTCGGCTTTAGAAAATGCTGCAAAAGATAATAAAACCTCCCAACCACAAGCACGATCACTTACAGCTTCTGATCAAGTTCAAATTACACAAAGTTCAGATAAGGTTATTGGTTACATCACCACATCTAATTTGGACATTGACAACAACAATACCTTTGATGTTGGTAAGTTAAACGGTGATAAGTCAACCAGCAAAATCATTGTTAACGCTACTTTAAAAACCTTAAACAAGATCAACACCTTGCAAAGCGAAGAGGGCATTATTCTGCCCCATCCACAAAAATATAAGAGTACTGATCCACAAGCTGTCGCTACCGTTCAGGGTCCAAGTATTATTGGTGTTCATGCTAACGCTAAGGAAAACGCTGAAACCCAAAAATTTATCAACTGGTTTATTAACCAAAAAGAAACTTGACCTGAAAATAGTAAGGGAAATAAGAATGGCCAAAATTGTCAAATGACCGCAGCACAATACTTTGCTAAGAGCTCTGGTTATGTTTTACCTTACAGTGAAACGTTTACTAAACAAAGTGAAGACGAACACAGCAAAACTAAAGACGCTTATAAGATCTTGAAAGATGTAAACGATGGTAAGTTAGTTGGTTACAGTGATCCAAGTGATTTCCGTTCTGGTAAATTCCGTGACACAATTGTCGCTGCTTTTAGTGGTGCTGTAAGCTCTAAAGCTGACTTTAATAAGTTCTTTAAAGATTTTGAACAACAATTAGGTCAAGAATACAGAAGGGGTTAATCCCACTTCTCCCCCACCCTTTCCCGGTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTTGACCAGGTGTTGGATTACGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAAGAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCCAACGTCCAAGCCACGCTTGGTGGCAGTCAGACGACGACTACAGGCGGCTCCAAATGACCCACCCTCGACCAAGCCAACCTCCTGCTCTGAACCGGCGCGGGGTGAAGGAATGATAAGAACGGACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGCAGTGGGCAGCAGGGTGGTTCTTCAGGTACCTCCGCGGGCAACCCCGACTCGTTGAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAAGTGTTGTGGATACGGGTCAAGGCGACGACACCCATTATGCTAATTTGCCACCAAGTATCACCCCCACATCCGACTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGGGTGCAGCTGTTCCTCCGCGGCCTGTTGGACAGCATCCCGGTGTTGGTGAATAAGAGTGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGCACCAAAAATGAAAGTACTCCGACTTAAAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGCTGTTGAATCCGGCCTTAGTTGAAACCTATTTTGGCACGACACGAGCGGGTGGTTCGGGGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTTGGTAACCTCGTTGTCAGTGGCACCACGGTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACCGACTTTGTCAAACCCCGGTCGGGGTACCTCGGCTTGCAGTTAAAGGGCTTGGATGTGAGTGAAGCGACGCAACGGGAGTTAATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGCTGGGCCGGGTCGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAACTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTGGTACCACCACCGCAACAGGGGCTGCCTTACCGGAGCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCATTTGATTAAACCTAAGAAAGTTACCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGCGCCAGAATGCTCCAATCATTTGGTACAGAAAATTCCACCCAAGCCCAGCCCCAGTCGCTCAAAACAACGACACCGGTGTTTGGGGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTACCACGCAGGGCTCAAGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAAGAACTTTTTTGAAAAAAGTTTTGAATTTATTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGTTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTATTGGCGCTTTTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGTGTTACTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAGGAATTTAACGAGATGATGAAAAAGACCAAAAAGGGCACTTTTTCGGGATGATACATCATTAATCCAGAAAACAACAGTGTGGAATTCTCTGGCAGTTTCAATAGACAATTTAAACTTAACAAACCGATTATCCCAGTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTATAAAGATTGAAACCACTTTGACCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGTTTGGACAAAATTGATCCACCCAAATAGTTTTAGGCTGAAAATAAAAATACTAGCAATTAGTCTTAAAAACAAGCTGTTGGTATTTTTTTATAGATAAAAAGTTAACTGTTTAAAAAAGCAAATGATTAAATTTTATTCAAATACAAATAAGTAAGTAATCACGATGAATTACTTAATCAAATGCTGTAAGTAGGTGAATAAATTAATTTGTTTTTCGTTAGCACTGTAAGTAATATAGTCTTTTAAAAAAATTTTATCCAGACTTTTTCTGCTAAGTTCATCTTGTCCTTTTAATGATCAAATCAGTGAAAGATCAAGCTGAATCCATTCTGTAATTATTTGTTGACTTTTATCTTGATCTTGAAAATTTTCATCTTTAACAATGAAACTTTTTTCTGTAAATTGTTTTTTTGAATGAGATTTAATACTAAGGAAAAGATCGAAATTATGCTTACCGCCATTATTGGCAGTGAAACTTTTTTCTCCAAAATAACTCCCATACCAATTAAAGATATAGCTTAACCTAGCATTAACTGAAAAGTCACTAAAGCTTTTATATTCCATATTGAAATACTCAACTTTCCGCTCCATTTTAAAATCGCTATTTTTTGTGGGGTTAGTTGCTTGATCGAAAGCAAGTTCCGCAATACCTAATTTACCCTCTTCAAAACTTTGTTTTCATGCGTCTAACAACAATTTTGTTGTATCTTTCGGATTAACTTCGTAAGACTTCTTTGAAGAAGTTATTATGTTCTTTTTACCATCTGTAGTTTTAATTGATTTAGACAAATCAATGTCTTTACCGGTGGCTTCTTTAATAAGATTTCTAAGATCAGCTTGGATACCAGTGCAAGCGCTTAAAATTGTTCCACATAACAAAAATAAAGAACCAAAAGCAAACCTTGACAAAAATTTCTTTTTGCGCATCAACAGTAAAAATAGTTCATCAATTCTGCTTTTTTTGGAAAAGCACGCTCAAAAAAGAACTTCATTGAAATTTTCGGTTGATTTTTTTCTTAAAAAAGAACTTTTTTAACTTAGAAAATTTACTTTTGACATTTTTATTCACATAAATAAAAAAAGCCATCTGCCCAAGGTGAAAAAAACAGACGGCTTTTTGACGATTTTGAAACTAACAGCAAGTTGTAACTGTTAGTGATTTGAATGAAAAAAATATATGACCTTTAGTCTTTTTAAAACAAATAAATCAAATGTGCTGAAAGCCATTACGTTAACATAAGTAAATTAAGACATAAAAAATGAACACATTAATCAAATAAGTGTGGGAACAGAAAATCAGGCTTTCAACACAAAATTTTCGTTTTTCTGCTCCCACTAAATAAATTGAGTTGGTTTTTAAAAACCGCCAAAAAGATTAAAGTTTTAACCCCACCGATCCTTATCGGTTTGCCGTTTTACTCGTGCCTCGCAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCAACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGGATGCCCAAAAAGACGACCAGAAGTCCTCGGAATATTTAAGTGGGGAGGCGGGAAGTCAAGCGGGTGGATCCACCCAAGGTACTTCCACCACCACCCAACGTAGGGGTTCGTCAAATGAAAACAAAGTCAAGGTGTTGCAGGTGGCGATGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACCACCTCCAGTTCGGAATCTGGTCAATCAGGTACTCAAGGATCCACCCCAAGTAATCCGGGACCCTGAACCCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTGATCCTGTACGATGCGCCTTATGCGCGCAACCGTACCGCCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAAATTAATAAATAGGTCTATAGGTTATATTAGTACACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGGGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGAAACCAACAAGCCTCCCAAAAAAACCTCACCAACAACCCCGCCCTCAAGGCCGTCACCGGCTTTAAGCTCGACAATGGCCGCGCGTACCGGAAGTTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACGAAGGATGGCAAGGGGAAGGATAAAGATGGGTGAACCACTTCCGGGGCGAGTGAGGCCAAAGGAGATGCCCCGTTGGTGAGCTCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTTTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGTGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGGTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCTACAAATGAAGATTTGCCGAATGTAATAACACAGCTCTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGTGCGACTTGATGAGCGAAGACACATTTGAACTTCGGCACTGAAGTTCAAAAAAGCTTCGTTGAAAACCAGTTGGGGTTTAAAGATGACTCCAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGACCTCACCCAACCCGCCTACCTCATCACTGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCGTTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGATCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGAACCAAACCGCCTCGCACTACCTCCCCGTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGAAGAAGAGCGGAGCAGCGGGAATAATTAGCATTTAAAAAGTTCTTTTAAAACAAATTTGAAATAAAAAGGATTCAAGAAATAAATTTCTTGTTTATTGTGAAAAATTCAAAAAAGCAACAATTTTTAAAGTGATTTCGCTTTGATTACAGACCATTTTTCAAAGCAAAATTAAAAAAGCTTTTTATCGTTTTTTGCATATAGAAAATTCAGCATTTATTAAGCTCAAAAAATAAGAAAAGCAGCAATTTCATAATTGAATTAAGTTTAATTCGCTAGTAGTGTTAAAAAGAAAAAAACTGCTCCAGGGTTTCTTGAAATTTTTACCGCTTATCATTCCCGCAACCATTTTTGTTAGCTGTGCAAGACGTGAAAGTAATCACCTCATCTTCAACATTTCACTCGATCACGACGCTGATGCTTCTATTAGTAAGTTCTTTGAACTTTACTCTAATAATTTAAGTAAAAAACTAGATAAAAAGGTTACCGTTAGCTTCAACATTATTGATGACTCTTTTACCAAAATTAGCAACATTCAAACTGCTAAAGCTGACTTTGCTTTCGTTAATTCTCAAAGTATTAAAGATAACGGAATAGAAGGATTTAATCTTATTCTTCAAACTCAAACTGATGCATTTAAGGAAGACACCAATTTAGATTATTACAGTGACGGTCAGTTGAAATCTAAAGCAGAAAAAATGACCACTTTATTTTCAAAAACGCCATATAAAGATTGAGAAGATACCGCACAACAATGAACCGGTAGCCGCTATAACTTTTTGTATGAAACAAACAAACTAATCAATTTCTACAGAGGCATGATTTTAATCACAGGTAGTGAGGAAGAAATTAAAAAAATTAAAGAAGCTTGAGATCAAAAGAAATGAAGTGATTTCATGAATTATGGCATCGGTCATGGCTCAAGTGGTTCAGCTGGAAAGTTCCAACTACCTGATCTTTTATTAAGAAAGCACTTTGGCTCTAGTTACCCCGGTTTGCAAAATGCAATTAACCAAAACCCTGATAAGTTTGCTAATGTTAGAGGTCGAGAAATAGGTAGAGACAATAAAATCAAAATTGTCTTTGATGATGCTAATTCCTTTGCTTGAACTCACAACGACAAGAATGCAACTAATCACTTTTATACACCAACAGAAAATAATGGCAAAGGTGATTCAGAAAAAAGCAATAATAAAAATAATAAAGTCGAAATCTTAACCTATACTGATCCAATGCTGTATGACATTGGCATTGTAAGTGATACTTTAAGTGATCGCTATCAAAAAGCAATTGCTGAAGTTTTTGTGGAACTAGCTAAAACAAAACAAGATATTTATGGCCCTTCATATGGTTACAACGGTTACAATTTGATTACGGACCCTAACAAAGAAATCTTGGATGTAATTCATAAAACATATGGTTAATCCAGTCTTAGTTTTTGATCAAGTTTCCCTTAAATATAACGGAGCACCACTTTTAGAAAACATTAACTTTACAATTTCACCTGGTGAACACATTTGCTTGTTGGGTAAATCTGGTGTTGGTAAAACTAGTCTTTTAAACTGCATTACTAACACCAAAACAATTTCGAAGGGAACCATTTATTTCAATGGTATTGCTTCTAATAACAAGGATTACAAACAATTAAAAAACAGTTCAGTTTTTTAGATCAAGTACCTAATTTAATCGACACTGACTTCGTTTATGACGCCATTTGAAGGGAAGCGAAAAATAACCTGAAATGGTGACAACGGTTATTTTTAGTGGAACCTCAATCGCTAAGAGAACAAATTATCCAAATCCTTGAAGAGGTTAACCTAAAGGAATATGTCACTTACATTATTAAGGACTTGTCAGGTGGTCAAAAACAACGTGTGGAAGTAGCTAAATTGTTCTTTGCCAATTCCCAAGTTTTATTAGTTGATGAGCCCACTACTGGACTTGATTTAATCAATGCGCACAAAATCATGGAACTAATTACCCAATTTGCGCGCCAAAAAGCAATGACCTTAATCTTTGTGACCCATGATGTTGAGTTTGCGCTCAAATACAGTGACCGTATTATTGCTCTAAAAAATAAGGCATTAGTTTTAGATCAAGCTACAAATAAGCTTACCAAACAAAAGTTAATGCAAATTTATCATGACTAGCTTGTTTTTCTATCAAATATCAGATAAGCAAAAGCGTTGAAATTGGTATTGAAAGTTAGCTTTAGCGATTATTGTTTTAGTTGTTATTATCTACAGCTTTATCGATAACTTTTCCGGTTTTAACGTATCTGGTTTTAGGAATTTTAGTCGTAATTTTATCCGGTTATTCACCCCTGACACAGCACGTGATTACTTTTTGGGCTCTTACCTACTGCAAACCATTTTTTATGTCGTGAGTGGTAGTATTTTGGGATTTGTCATAGCCCTGTGGTTTTCGTACCTCACTGCCTTTAAAATCCAGCCACTTTACATCGCTTTACCAACTCGCTTATTCACCATTTTTCTGCGTTCCTTTCCAGTACTTGTGTTTGCTTTTTTGTTTAACAATTTGTTTAACAAACAACTAACAGCAACCTTAACGATTACCTGGTTTAGTTGATTGTGAAGCACTAAGTACATAACTGCTTTTTTTGAAAACAGTGCTTTAAAACAATTTTTTAACCAAAGCTCTAGATATATACATAAATTCAAAGCTTTTTGGAATACCGTTGTTATTTCCCAAGCTGAGCGCTTATGGTTGTTTTTGCTCTACAGCTTGGAAGCTAACTTTCGCTGAACAACAGTTTTGAGTATTGCTGGCATTACTGGAATTGGTGAGCTCATTGCCACACCTCTAGGGGGCACAGTGCAGCTTAACTTAGTTTTAATACCGATGTTAACTTTGATCGGCTTTTTACTGTTTTTAGAAGCGAGTGTTTTTCTGTTAACTAAGTTTGTTTTACAAAAACAGAGCCAAGCCGGTGATTACTTTTTACAAGCTAAAACACTGCAAAAGAGAAAGTGAAAAAAAGTGATGATTTACATCCTAGCTTTGGTTTTAGCGGCTTTTACCTTGGCTAATTTAGTACAGTTGGACTATACGGTTAAAGCGCCAGGGTTTGTGGCTGACTTTTTTAAACAGTTCTTTCAAACAAAAACAGCTTTTTTAATTAGTGAAGACGCTAACATTAACCCACTTTTAATGTTGTTAAAACTCACAACTCAAGCGATTAGTTTAATTACCTTAGTGTTTGTGTTAGCACTCTTGTTTGGCTTTTTAGCATCAAAGCTGTTTTCCACTATAACCTCAATTAGCTTAAAACTGTTACTTTTAGTGATTAGGGTAATTCCCAGTGTCTTACTGTTCCGCTTATTTGATCCAATTATTTTTCGCCCAGAAACTACCATTATCTTTGTTTTAGCAATCCACAGTGCTGCTAGTTATGGTCAACTGATAACGATTAACTTTGATAATGCCAACGAAGGGGTAATTAACAACATGCAGAACCACGGCTTTAGTAGGTTTTACATCTTGTGAAATTACCTCATACCAACCACTAAGCCCCAACTGTTAAATACGTTAAGTGATAGCTTTGATAATGCCATCCGCGACTTAGTTGTGTTTGGGATCTTTGGTGGTTCGATTATTGGGGTGCGAATTAACAATTTCTTTGAGCGAGCACAGTACTCTGAATTGGGCACAATAACCTTACCCTTAATGGTTTACTTAATGGTGTTTGAAGTCATTTTAATGGCAGTACGGTTAAACAAACTCAAAGTGTGACAGCGCCATCTGTGGTAGTTAATTGGGTTTTTGTTGGTTTAAGAAACGCTCTAAGATTAAGGTAGCCGCTAAGGTGTCCTTGGCCTTTTTAAAGGTTCTGTTTTTTAAGCCTAATTCCTGCAAGTTAGCTTTTACCTCACTCGTAGTACCACTTTCATCAATGAGTGTTACTGGTACATTAAAGCGGGTTTTTATTAGTTCCACAAACTTGTGAATGCTCACTTGGATACTTGACTGGTAGTGGAAGTGGGGAAAGCCAATCACTATTTGCACTAATTCATAACCAGCTTGCTTTAAATCCTTAAAAAGGGTATTAACGGCTTGTTGCAAATTATTTTGTACCGCAAACACGCGGAAGGGACTTGGAAAGCGGTCAATGGTATTGGCTAGTGCTGTACCAATTCTCTTCAAACCAAAATCAATGCCTAAAATGTACTGCACTATTGAAGGGTATCGAGGATAGCTTGTAGTTTACTTACATCCACCGACTCGTTAAACGAACCCCTAAAGAAGTTATTACTACCCCCACCCTTAAGTTCAAAGTGAGCTTTTAAAAGCTGAATACATTTAGAAACATCTTGCCCTAATAACATAAAGGACGGACTGTTGTTGAATTGGTTGAGGAGTACAAACAGCTTGTTTTTGTGTTGGTTCAAGACTGCTTGGGCTACTTGTGATAACAGTTTCGGATCAACTGCTGCAAAGCTAGCGAGAACATAAGCTTTGTCTTGCTTTGCTAGCTCTAACAATTCCTGTTTGATCTTTTCCGCTAACTGCTTTTGCTTCCACTTAAAGTCTTCCGTTTTCAGTTCATTAACCGTTATTTTGAGTTCATTTAGTAAAGCTTGGCATTTACGTAAATCAGTAATTACTTGCGGCAAGTGAAAGTGATTTAGTTGCTTAGTTAAAGCAGTTTTTTGCTTGTTCAAAGCGGGGTTAGCAATGTTGTTTAGTGTATTGTGTAAACTGTTAATGGTTTCACTTAACTTGCCATTCTGCTCATTGAGGTAAGCTGCAATTGTTTCGTTACTACTAATGATTTCAATCCGTCAACGCCCTGTGCCACGAGCATAGTAATCAGTAATAAAACAGTTCTCAATTAAACCAGTGTGTTCCACATGGGTACCACCACAAAGCTCGACACTAAAATCACCAAAGCGAATTACCCGTAACAACTCGTGGTTCGAATACTCCTCTTCAAAGTAGGCAATCGCATTAAGTTTTTGACTACCCTCTAGATCAGTAAAGATCTCTTCGCGCTGAATGTCTTGTTGAATAATTTGTCGAATCCTGTGTTCCACCCGTTCCAGTTCGGCTCAGGTTAAGGCTCGCGAAAAATTAAAGTCAATGGTAGCTTTTTGCGCTGACTTAAAGGCGCCGTCTTGTTTAATCAAGGGGTCAATTTCCTTTTGCAATGCCGCGTGCAGTAAGTGTTCTAAACTGTGGTTGTTTTTCACTAGTTGGCGCCACTTACCGTCATGTACTAGGATCACTTTGTCACCTAGTTGAAAACTACCAGTTAAAAAGAAGTGGAAGTGTTGTTTATTAGGGCCTTTAAACACGCCCTGAAAGCTTACTCTTTGATCATTTTGGCTGTGGTTAATACAGTACCCCTCATCATAACGCTGACCGCCTGATGTGGCATACAGCACCGTTTGGTCAAACACCACATAACCACTTTGGTTGTGCAGTTTTTGCACCGGTTGGTACTGTTCATCAAACAGCCCAATGACCTTGGCGGTGATCTCATTAGCCTCGTATAAGAACTCACTCGCTGTTTTAAAGTTCACTAAGTGTTCATTTTGCTTTTCAAAGCTCAACGTATTACTGTTTTGTTTGGAGATCAAACGGTGCTGTTCCATCAGTTGTTCCACGGCCTTTCAGTCTACCTGGAGTTGGCGCTTATCCACCAGTTCCTTAATCATTTCAATCGGAAAGCCATAGGTTTCGTACAACTGGAAGGCTACTTGGGGTTTGAGTGCATTATGAGCAATACTCTTTTCAAACAGCATTAAGCCCAAGTTAATTGTTTTATCAAAGGCCGTAACTTCCTTTCAGATTGCTTGTTGCACCACCTGGTTTTTCCCCTGCAACTGCTGGTAGTAAGACCCATAAACGCTAATAATGGCATCGACCATCGGATCAATAAACTTTAAGTCTAAGTCCAGTTTTTTGCAGGCCAACAAAGCGCGGCGCAGTAACCGGCGCACAATGTAACTGCGCTCGTTGGGTCCTGGCAATACGCCTTCGGCAATGGTGAAGGTAATTGCCTTAAAGTGGTCACTAATAATGCGAAAGGCACTTTGCGCTTCGTTTAACTTTTGGTCAAACTTAAAGTATAAGTTGGTATCATACTTATGCTTACAGTGCTGTTCAATAATGCCAATTAACTTTAAGAAGATATCCGTGTCAAAGTTAGTCGGCGCGTCCTGTAAGATTGCTACTAGGCGTTCAATACCGGCCCCAGTATCGATGTTCTTCTGCGCTAACTCACTGTAGTTACCATTGCCATCATTGTTAAACTGACTAAAGACAATGTTCCACACTTCCACATAACGGTCGTTTTCAATGTCCTCAAAGAACAGTTTTTCACCAACCTTGTGCGGATCAAACCGCTCCCCGCGGTCATAGTAAATTTCCGTACACGGTCCACATGGTCCTAAACCTAAGTCCCAGAAGTTGCGGTCGCGGTCACACTTAATAATCCGACTTGCAGGAATACCGTGTTTAAGTCAGGTGTTAAAGGTCAATTCATCATCTTGGTACACTGTAATGTAAAAGCGCTTCGGATCCAAGTGGTAATGATTGACTAAAAGATCATTGGCAAAGCCAATGGCCTCTTCCTTAAAGTAATCACCAATCGAGAAGTTACCCAACATCTCAAACAGCGTTTGGTGTCGCGATGTAAAACCGACGTTTTCAATGTCATTAACCCGTAGACAAATCTGGGCGTTGGCTAACCGTTTACTCGGTGGTGTTTTCTTAGCACTAAAGTAGTCCTTTAAGGTCGCTACCCCGGAGTTAATCCACAGCAGTGAAGGATCATTTACGGGAATTAACGATTTGGAAGGGAGTGCTAAGTGGCCCTTGGCAGTAAAGTAATCTAATCAGGTTTGTCTAACCTTATCAGTTGTTCATTTCATTGGTTTAATTGTAAATGTTTGCCTTAAACCAAGGATGACCACGCAACAAGCTTACACTTCAAACTTCTTGTTGGCAATTTGTGCATACACCATTCGATCTTGACGGAACTGGTGGAATTTAACCTCACTGTTAAGCGTTCACAGCCCTAAAGGTAACTGCAAGCTCAACACCATGTCCGGGAACTTTTCGTAAATGTTTGTTCAGGGATGGAGATACTGACACACTTGCTTAATTTTGAGTGCATCAACTGCTTGGAACTGCTTTTTCGTTCAAAACAAAAAGGCTATTTGGTAGTTGGGGTTAATGTCATACAAGCGCTGTAAGGAAGCAAAGTTAAACGAGGAGAACACTACCTTTTCAATTACTTTTTTGCCATACTGTTGGGCCAAAGCATCAATTTTAGCTTCAATTCCCGGGTATTCCTTTTGGTCAGTTTTAATTTCCACGTTAATTAGTTGAAATTTATCTAAGTACTGATCAAAGAACTCCTTTAAGGTCATAATCGGCTGGGGTTGCGTTTTGAACTTAAAGAAAGCACTGTGATCATCTTGCTTTAAACTAGCGAGTGTTTCCAGTTCGATTGTTTTGTCGACTAGCGCTGTTCTGGTGGTAGTTTCATCATGGATAATCACTAACTCCCCGTCCTTGGTCAAGTGCACATCTAACTCCACGCCATCAAAGCGATATTGGAACGCTAATTCAAACGCTAGCTGGGTGTTTTCAGGCGCAATATCACTGTACCCACGGTGTGCTAGCAGAAGTTGTCGTTTAAGCATTGTCTAAGGTTCTCCAGCGGTAATCGGTTTTACCCAAGCGCTTTAACTCTCAAGTATTAACCAAAAAGACAATCGTACCACACAATAAACCAAACAGGGCTATTCCTAGACAAATAGCTGCAATAATTTGGTAACCCTGAATCGAGGTATTCTTCTGACCGTCTGGGGTATAAGCCTTACCCACGACACTTGTAACCGTGTAGAGCCAACCATCAGTCGAAAAACCAATAAAGGAAAGTAAACCAACACTAGAAGCATAGTTGTTTTTACCAATCTCTATTTCCGCTACCTGGTTGTACCTAACGGTTACCATACCCCAAGACAACACCCCGGTGAGAATGTAGAGGATCGCCGATACGATAATCAAGGCATAGTTTTGCTGTTGGCCCACTTGCCCAAAACCTAAGAGAATAAACATCAAGACAAAGATAATGCCAAGTCCTGTACACAACATTAAAAAGAGGATGTAGCTCCTAAACTTATCAGCTAACCTGCCCAAGTACACACTTACAGCACTGCGCAAAACATACGTCCTTACACCACCCAGTACAGTCCCTAAGATCACTGGTGCCAAAAAGGCGTTTTGCATCATTTGTAAAAGATAATAAGCAAACGTACTTTGAAAGACATACATTCCCATTAAAAAGAAGGACAAGAGTCACAACTTCCAGTTCTTTAAGGTAATGAGAATCTGTTCAAAGTTCTTTTTGGCAGTTTTTAAACTGGTATGACTGTCGTACTTTTCAATTGGTTTTTCTGGTACAAAGAACAGCACGGTAAACCCAGTAACGAGCAACATGCCACCAATAATAAAGGCATAAATGGCAAACGGTGCGGGATTATTAGCACTACTTTCACCACCGGCTGGGTAAGCTACTGCTGTAATAATCAAGGCAATTAAGAAAATAAAAATAAAACCCCAAACACCATTAGCAGCGCCCTGAATACCAAAACCCAATGCTTGGTTTTCCTGGGTTGCTTGTTGACTCGCTAACTTTCACAGTGGTGTTCAAAAGATTAAGGTCGAAGTAATACCCCAAAGCCCCCAAATAACACAGTACTGTACAAACAAGGCATTGGATTTTTGGCTTTCACGAGTAAGAATGTTAGTAGCTAGCCAAAAGGTAATTACACCCGTTGAAATTTCTGACAAGAACAATAGTTTCCGCGAACTAAACCGGTTCGCTAAAAAACCACCAGGTAACTGCGTGGCTAAGGTCACATAACCAATAATCGAGGTTAAGGTGGCCACTTCATCTTCAGTAATCCCTAAATACAGGTGCAAGTTAGGCACCACATTCTTAATATAGTACGGGGCCGCAATTACAAAAACATCAGCCGCACCGAGAATAATTAGTGCTATGAGCTGCTTTTTGGAAAACTGCTGCAACCGTTTCTTTAAACTAAGTTTCGTTTCCATTGTGACACAACGAAATTACGCCATTATTTTAGTGGTAATTATGGGGTTAATCCGGTTAGCTTGCCAAAAAATAAATTAGAAAAACGTTTTTGGCATACATTTATTGCAAAAAGTGAGAGGAAAACCGTATTTAGATTTGGCTAGCGGTAATGATACCACTCCCCAAACAAATGCCATTCTGGGTATACAAAACACCATACTGACCGTTGGTAACGCGGTCAATTGGTTGATCTAGTTGTACTTGCACGACGTTGTTTGGTTGTACTGTTATGTGCCCTTGCACTAACGGTTGGGCATGGCGAATCCGTACCAGAACGGTTTGGGTCAATGGATTGGGTTGGTACAACCAGTTAAAGTGGGAAAGGGTAATGCCACGACGCTGTAAATCTTCTGGGTCACTCGAGACATACAAGGTGTTTGTTTTTAAGTCCTTTTCAACGACAAAGTTCCGTGCTGCTTGCCCACCTAAATTTAAGCCACTGCGCTGCCCAGTGGTGTAAAATCACACCCCTTCATGACTGCCTAGTTGGCGCTGCGTTTTTCAATCATAAATAACCCCCGGTTTGACTGGTAAATAATTCTTTAAGAAGTCACTAAAGTGGCGTTCCCCAATAAAGCAAATGCCGGTTGAGTCCTTTTTAGTAGCCACACTAAAGTGGTGTGCTAACGCTATTTGCCGCACCTCCGTTTTCAGTAAATGCGCCAAGGGAAACACCACGTCTTGTAGTTGCGCTGGTTTGACATGCGCTAAAAAATAGGTTTGGTCCTTGTGCTTGTCTTTGGGAATGGCCAGTAAAGGTTGGTTGTTGTCCCAGCTAAGTGCCGCATAATGCCCTGTAGCAAAGTCACTGTTGGGATCTAACTGCTTGCAGTAATCACGCATTAGCCCAAATTTAATTAAACGGTTACACAACATGTCGGGGTTAGGGGTTAACCCGTTTTTAAAGGCATCAATGGTAGGGAGAAATACCTGCTTTCAGTACGGTTCCACCAGGTTTTGCTTAATTAGTTTGATTCCTAGTAATTGGGCAATTGCTTGCGCTTCGCGAAAATCTTCTTTGGACGAACAACCCGACTTATGCTCGTTAAAGGCGCGGTGACCTAACTGGTCGTTATTTAGTGTATTGTCCCAGCACTCCATAAAAATGCCAATCACTTCGCGGTACTGTTGCTTTAAAAGGAGCGCACTCACAGCTGAGTCCACTCCACCTGATATTCCGACAAAAACGGTTTTATTTTGCATCTAGTAAACTGTTAAAACACCAAAGCGCTTACCCAACTGCTGGTGGTAACGCCCGACATCAACACCCTTAGTTATGGCCAGCGCTGAAATAAATAAGGTTAACGCTTCATAAAAGCTCTGACAACAACTTTTGACATCACCTTTAGGATTGTCCACTAAAACAGCTAACAAATCCAGTTGTTTGACCACTTCACTACTAATAAAAAAGGCGTCACTGAGCATCGGTTTTAAAAAGAAGGACTGATCATCATAATCATCATCTTCATAAATTAACAGGGTACTCTGTTCGCGTTCCAAACAACGATTAAAGTTTTGGCGAAAGGTCTCAAAATCAACTTTTTCAAACTCAGTTTGCACAAACTGCTTCAAGGCTAAAAGTTGTGGTTTCATTACCTTAGTTTAATAAGTTGCTCGCGTAATTGGGGATCTGTTATTTTTTCGTACAACTTTAAACGGAGCTGTTGCTTTTTAAACAACTGCAATTTCGCTTCAAAACGGTTAAAGATCAGGTTGCAGGTTTTGATGTGTTGGTGGATTGAACTTTTCTTAACTTGGTATTTATCAGCAATCTCTTGCATGCTCAAATCCAGGTTAATGTACTCGTTAAAGTAACACGCTTGCCGTTTGGTTAAAAGTTCCCCGTAAATATCAAATAGTTGCTGTTGCTTTAAACGCTGGTTAAGTTTGGTTTGGTTCATGGTTGAGGCTTAAATTCTTGGTAAGACCCAGTACATATTGCTCTAAATCAAAGGCCGCTAAATCAGCAGTTGTTTCTCCAAAACCAATCAGTTTTACCGGTAAGTTAAACAGGTCTTTAATCGCTAGGATAATCCCACCCTTAGAGGAGCTGTCCATTTTGGTAAGGATAATCCCGGTAAGCTCGGTAAATTCATTAAAGACCTTGGCCTGTGCTAACCCCGATTGTCCGGTAGTACCATCTAAAACGAGCAGTGTTTCTTGGGGTTTAGCGCTACTCACCTTCTGGACAATTTGGTAAATCTTTTTCAACTCATTCATTAAGTTGGTTTTGTTTTGCAACCGTCCCGAGGTGTCACACAACACAAAGTCGTATTCGTTTTGAATGCCTTGTTGCACCCCACGGAAGATCACCGCTGGCGTTTCCTCTTTGGGATTGGGTAAAACAATGTCACAACCTATGCGCTGCGCTCATTCTGCTAACTGGGCCACTGCCCCAGCGCGAAAGGTATCCCCAGCGACCATTAACACCCGTTTGTTTTGCTTGGTAAGCTGGTCAGCTAGTTTGGCTAATGAAGTTGTTTTGCCAACACCATTAACCCCGACAAACAAATACACGTTGGTTTTATTAGCTTCAACCGTTAAATCAGTTTCAAACAACTTATCTTGGATATAGTAAACAATTAACTTATCGACAATAATTTCCTGGATTAACTGAGGATCACTCACTCGTTGTAGTTTGAGTTCTTGGACAATCGCATCACAGATTTTAGTAGCCGCATGGTAACCAATATCAAGCAACACTAACTCCTCAAACAAACTTTCTTGAAACTGTTCGTTAACCGTTACATAGTTAGCTGCTAGTTTATTAATGGTATTGGCAAAGCTGTTAGCCGACTTGTTTAAACCCTGGTAATAGCTCTTTTGGTTGGCTTGAAAGAAGCTCTTTTCCTGGACAGCTTGTTGCACTTGGTCGACCAGTTTCTTTTTCGGTTTAAACTTCTGGATTAGCTTGTGGATAAAACTCATAACAATTAGTTCTGCTTATCTTTACTAATGTACTGCTCTGCTTTTTCCAAAGAAACGGCAAACGTCTTGGTCACTCCCTTGGTCTGCATTGCCGCTCCCAGTAACATGTCACACTTCATCATCGTGCCCTGACGGTGGGTAATAATTAAGAACTGGGTGTTGTTACTGGAATTGCCAATAATGTTAGCAAAGCGCTCCACGTTCGCCGGATCTAAGGCACTCTCCGCTTCATCTAAAATTACTAGAGGAAAGGCACTAACTCTCAAAATACTAAAGAGGACCGACAAGGCCACGAGTGTTTTCTCCCCACCCGATAACAACATTAAGTTAGCGACATTTTTGCCAGGCGGATTAGCAAACACTTCTATGCCTGACAACAAGACGTTATCAGTATCCGTGTAGCGAATTTGACACGAACCACCGCCAAAGAGGTAGTTAAAGGTTTTAGGTAGTTCTGCATTAACTTTTTGGATGAGCTCGTCAAATTCCTTGCAGGCCAATTCATCAATTTCACCAATCGCGGTTTGCAAATCCTTAATCGCCGTTTGCAGTGATTCATACTCACCGTTAATGTCATCAAAGCGCTTTTGCTTATCAGCAATTTCAGCAATCGACTCCAGGTTAATTGGACCCATTTCATCGAGTTGGCTCTGCATCCTGGCAATCTTGTTTTGCGCTTGCATGGTGGAGAGCTTAATTGGTTTGTTATAGTTAGCGATCGCAAACTCCATCGTTAGTTTATACCCACCGTTAATCTTGTCGGTAATGTTGTGGATTGTGTTTTCAAAGCGGATTTTGCCTTCGCGTGCCAACACTAACTGGGAGCGTTGTTCATCTAAAAGGGCACGGAGGTCAGCGGTTTTGGCTTCGGCTAGTTTAATCGATTTGGCCAGTGTTTCTTTCAATTCCTGGTTGAGCTTTAACTGTGAATTGAGTTCGTCACGTTCAGCCCAAGCACGGTTTAATGATTCAACCAATCTGGTTTCATCAAAGCTGTGCGGGGTTCCGTCAAAGCTTTCGTTGGTGAGCTGCTCATACTCAATCTTATAGCTCAAAATTGCCTTATTGGTGTGGTTAACACGTTCGTTAAACTTTTCCAGTAGGATTTCTTCGTACTTTAACTTTTTGTTGAGCTCGTTGAGTTTAGTCTCTAAGCTAGTGAGATTGGTACGTAGTTGCTTTTCGTTATGTTCTAGTTGGGTAATGTTAGCTTCACTAGAAGCTAACTTTTCCTGGAGGTTATACAAGCTTAAGTTGTCCTTGACATAACCACCCTGGATAATCCCTCCAGCGTACACCGTTTCCCCGTTGAGGGTGACAACGCGGTACAGCTTATAGGTATAACTGGACAGTTTTAAAGCGGCTTGAAGGTCACTGGCAATAATTACTTGACCCAATAAAGCATTAACAATTGGTTGTAACCTTTCATCACAACTCACATGGTCACTACCAACACCCAAAAAGCCATCGAGTTGTTGCAAAATTTCCATGTGCTCGGGAACTATCTTAGTATCAAAGGCAATGTCGTCAATTGGTAAAAAGGTAACTTGACCAATCTGGTTAGTTAACAAAAAATCAATTGCCTTTAAAGCCGCCAAGTTGTTGTTAACAACCAGATAACCAATTGATTTACCCAACGCCTTTAACAAGGCCTTTTCGTACTGCTTTTCAAACTTTAAAAAAGTGTTGAGCGTGTTTAAGAGACCGGTGAGGGCCTTGACATTTTTGACCAAAACATGGGCCGGATTCGTTTTGACCCACTCCTCGTTTTGCGCTTTTAAAAACTCTTGCTGCGCTTGTTGGTAGACTAACGCCGCACTGTTTTGGTTAAGATTCAACTTTGTGGTGTCCAACTCAGTTTGGACGGTGCTCAACTCATTGGTTAACAGGGTAATTTCACTGGTTGTTTTGAGTACTTGCGCTTGTAAGCTTTCTAGTTGCGCTTGGTCTACCCGGATTAACTTTTTTAACGCCTGGATTTTGTGCTTTTCGTCCTTTTTGGACAACTCGTTTTTGAGCTGCACATCAATCACGGCCTTGCGTTGTTCCAACTCGTTAATCGTTTGGTAGACCGCTTGCAGTTCTTGTTGTAGTTTGATGGACTGTTCATCAGCATCACGAAAGCGCTGGTTAAAGATGTTGAGCTGGTCTTCTAGCAGTTGTAACTGCGGTTCGTGTAGTTTAAAGTCCTGTTCGATGTAACCAATTTGGTGGTTAAAGCGGTCTAACTCACCCTGGGACTTGAGGTAATCTGCGACCAAGACACTCAGCTCCAGCTCCTTTAATTCTTCCTTAAGTTTGACAAAGCGCTGGGCCTTGTCCGCTTGAATGGTAAGCTTCTTTAAGTCCTTTTTGAGTTCATTTAAAACAATGCTGACCTGTTTGAGATTTTGCACGGTACGCGCTAATTGGTTGGTAACCTCTTCTTTGCGCTTAGTGTACCGCCCAATCCCCGAAGCGTCCTCAAAGATCTTACGTCGCTCCTCTGGTTTGGCTTCCACAAATCACGACACACTGCCCTGGGAAATAATCCCCAACGAGCCTTTTTCCAAACCAATATCAGCAAAGATCCCACTAATTTCTTTTAGCGTTACTAACTCGCCGTTAATATAGTACTCACTCTGACCACTACCACGATAGATCCGGCGCATCACCGAGATCTCTGGTCGGGGATCGTGCAGTAGTTTTTGGGAATTGTCAAAGGTCAGTTCCACTTCCGCTAGCTTACTAGCGGGCTTGTCCTTAGAACCAAAGAAGATCATATCATCACCGGACTTACTGCGCAAGTGCTTCATGCTGCGTTCCCCTAAGACCCACTTCAAAGCATCGACAACGTTGGACTTGCCAGAACCATTAGGACCGACTATACCGGTCATTGAGTGGGTAAAATTAATTGTTATTTCGTCAGCATATGACTTAAAACCATACGCACGAAACCGTTTGAGAAAAACCATTTAAATCAGGTTAGATTTTGCAATAGTGCATTACTAGATTATACTAGTTGGCTTTAAGGAAAGGTTTAGCGCCCCTCAACTAGTTGCACCAACCACTTGTCTTTGGCAAACTAGTACTTGTAATACTTACTTACAATCTAACTATCATGAGCGGTTCAGTTAAAAAAATTAAGCTTAATTAAGTATGACAAAGACAAGTAAAGCATCCGGGTTAAGCTGGTTTTTTTGTGATTTAGATGGCACTTTACTGCGTTACCAAAACAACCAGCACTTAATTGAACCAACTACGAAGCGAGCAGTGGCACAGTTAGTGGAGAGTGGTGCCAACTTTGTTGTAGCTACTGGACGCAAACCGAGTGACGTGCGCAACATTTACAAGGAGTTGGGGATTGAACAAGCCAGTCCCTACTTAATTGCCAACAACGGCGCGGTAGTGTGGGATTTAAAGCGCAATAGCTACCTCAACAAGCAAACCTTGTCTTTAAGTGACTTTGACTTAATTGACCACATTAACCAAACGTTAAACCAATTAAACCACGAGTATGGCTGCATTCTGTATGGCCTCAACGACCAGGTGTACTTTTATCATATCCACGCTCCTGACTCACAGGCCTTCAAACAGTACTTTGCTTTTTATGAAGGCGAATTTGTCCAAAACCAGTATCTGGAAATAGATGGGCTCAAAACTGAATACAATCTAGTTAAAGCCATTTGGTTTTTTAAGGAAGTGCACCAGCAAAAGGCGGTGATTGCGCAACACTTTACTAACCAAGAACGCTTGGTCATTACTTCAGCACACTCGTTTGAACTAGTACCGCTTAATGTATCAAAGGGTCATGCGATAAACCTAATTAAACAACAAGTAAAAATCACCGATAACCAGATTATGGTCCTCGGTGACTCTTACAATGACTTGCCAATGTTTCAACATGGTGTAGTTAAGGTAACTAATCACCTCGCACCCGACAACCTCAAACAACTAGCTACAAGGGTTTATGACCTACCAGCTTCACTGTTTGTGGGTCAAGCGTTGAACGACTACTTTAAGTTCGATTAACTTATTTATTTAAGTGTTTGCGCTGCGGTAATAATGCCCACGTTAAAGACATCACTGACACTGGCACCACGGGAAAGGTCGTTAAAAGGACGTGCTAGACCTAAAACAATTGGACCAATTGCTTCATAACCACCTAAACGTTGCGCAATCTTGTATCCAATGTTACCAGCATCTAGGTTCGGGAACACATAGATGTTAGCTGATCCATTCCAAGTCAAATTAGGGGCTTTTTGTTTGCGTACCTTTTCCACAAAAGCACTGTCAAACTGTAATTCACCACAAACATTAGCTTTGAGTTCGGGGTGTTTTTCCAAGAAGATTTGGGTCGCACTGACTACTTTGTCCACCGCTTCCCCTTTACCACTGCCCAGTGTTGAATAGCTCAAAAAGACCATCTTGAGTTCTGGTTCCCCCATACTTTGGGCAAACTTAAAGGTGTTTTCCGCTACAACTGCTAATTCTTGGGGTGAAGGGTAAACAGCAAAAGCACAGTCGGTAAAGTACAAGCGTTCCTCATTCTTTTCCATGATGAACACACTCGACACAAAGTTACCAGTACCTAACAGTTGTAAAGCAGGACGGAGAGTATCCTTCGTAGCGTATTCCTTACCACACACTTCCCCATCCACTTCGTTGAGTGCTACTAAAGTGGCTGCTAGGGAACTGGCATCACGGACAAACTTTTGTGCTTCACGCATTTCCATGCCCTTGTGCTTGCGCTTTTCATAAACGAAGTTAGCGTACTTTGTCAAGTCCATTTCCTCAATCACATAGTGCTTGATGGCGGTGTTAAAACCAGCTGGTACTTCACTGCGGGTGCGGAAGATCACCGCTGGGGTTAAAATACCCGCTTGCTGGAGCTGGTTAACTGCTTCCATTACGGTTGGCGATCAACCTTCAGGGAAAATGATCACTGGCTTTTTACCAGCACTTTGAACACGTTGTTTTAAAAGATCAATAACACTCATAAAAACCTGCTTACTTAGTTTTACTCTTTACTTTAATTTACTTTTTTTGAATATCGCTAATTCCCACTAGCTCTTCCCCACCTATTAGTGCTAAAGATGCACCACCACCAGTGGAAATAAAACTAAATTGATCAGCAATCTGGAGTTGTTTAGCAGCGGCTGCAGAATCACCACCGCCAATGACACTAAAGGCTTGTTGGTTTTGCGCAATCGCTTTACCAATTGCTAAGGTACCCTGAGCATAGTTGCTAAATTCAAATACGCCCATGGGACCATTCCAAAAGATTGTTTTGGCCTTGGCGAGGTAACTGCTAAACAGGGCCACTGTTTTGCTACCCACATCCAAGGACATAAAGCCCTGAAGTTCTGCTGGTCATTGGGGGGCTACAACACACTCCACGCCTGGTTCATCCTTAAAGCTAGCATGAAGCATTTGGTCTACCCCCAAGACAATTTTCTTGTCCTTGTCACTGTCAAGGATTTGCTTGGCGGTTTGGACTAAGTCTTCCTCCACTAAGGAAGTGCCAACTTCGTTACCCAGAGCTTTGAGGAAGGTGTTGATCATGCCCCCACCTATTAAAATATGATCCGCAATCTTGAGCAGGTTATTTACTACCTGTAACTTATCAGAAACCTTAGCGCCACCCAACACAACCACAAACGGTTTGGGTGGGTTATTAATTAAGCGGTAGAGGTTGATCAATTCCCGCTCCATTAAAAGACCAATGCAGGAGTTTTTGATGTACTTGGCAATGCCAGCGTTGGAAGCGTGACGGCGGTGGGCGGTGCCAAAGGCGTCATTGACAAAGATGTCACCCAATGATGCTCAGAACTGTGCTAACTCAGCGTTATTCTTGCTTTCGTGTTTAACCACTTCACCAGCCTCATTCACATCACAGTAACGCGTGTTTTCCAACACCAAAATTTCCCCTAATGGGAGTTGATTGACTGCGACTTTGACCTTGTCACCCGTGTTTTCCGGACAAAAGTGAACCTTGGTGTCCTTTAATAAATTTTGTAAAGCACTAGCTACTGGTTGGAGTGACTTCTTACCCTTTTTGTCATCGAGCGATTTGACTCGTGACAAATGGGACAGTAACACAATTTTGCAATTGTGACCCAAGAGGTACTTAATCGTGTCTAAGCTAGCTAGTATGCGTTCGTTGTCGGTAATGACCCCGTCCTTCATCGGCACATTGAGGTCAGCACGTAAGACTACGGTTTTACCCTGAAAGTCAAACGCTTGGACTGTTTTAAAGTCAACCATGATTAAAGCTTGGCACAATAGTTAACTACCCGTACCAACTGGTTTACGTAAGAGGATTCGTTGTCATACCATGCGTAAACCTTGTAAAGCTTGTTACCATCCACTTCAATAATGTTGGTTAGCTTGGAGTCAAAGATGGAACCGTGTTCCGAGCCGACAATGTCACTGGACACAATTTCATCCTCACAGTACCTAAAGGAAGCGGAAGCCGCCTTCTTCATTGCTTGGTTAATTTGTTCTACGGTAGCATCCTTTTCAAGGGCTACACAAAGTTCAACGATCGAACCAGTTAGTACGGGAACACGAAGGGCCATACCGTTGAGCTTACCAGTAGCTTCTGGCACTACTAGGCCAATTGCTTTGGCAGCTCCAGTGGTGGTAGGTACAATGTTGCAAGCAGCAGCACGGGCACGACGAAGATCGCTGTGGGGTGCGTCTTGTAAACGTTGGTCGGCGGTGTAAGCGTGTACCGTTACCATGGTACCGTGTAAGATGCCGAAGTTCTTTTCCAAGACGTGTACCATTGGTGCTAAGCAATTGGTGGTACAGCTAGCAGCTGAGATAATTTTGTCCTCAGCGTTAATAATCTTGTGGTTCACGTTGTAAACCACGGTTTTAATTGTTTTTTGTTTAGCTGGAGCGGAAATAATCACGCGTTTAGCACCAGCTTGCAAGTGCAAACTAGCACCTTCTTCACTAACAAAGCGACCAGTGGATTCAACCACAATATCAATATTGTGTTCTGCTCATGGCAGGTTAGCAGGATCTTTTTCACTAAACACTAACACCTTCTTTTTGTCAATCATTAAGGTGTTGTCCTTAGCTACTACCTTTTTCTTAAACTCACCGTGAGCGGAGTCGTACTTTAGTAAGTGTGCTAAAGTATCGGGATGGGTGAGGTCGTTAACGGCAACGATCTCAATATTTTTTTGGCTTAAAAGTGCACGAAAAACAAGGCGTCCAATACGTCCAAATCCATTGATAGCAACACGGATAGTCTTACTCTTTGCTAGCATAATCCTCTAATTAGTTTATATTTTACTACTTTAACCTATGGCGAGTAATTCTAGTTGCCAAAAAGCTAAAAGTTGTGGACTGGAAATTTGCTTAAAAAAGCAAAAGTTTTGGTGGGTAAAGGTAAAGCTGTAGTGCGGATTGGTTCAGTACACCAAGTGTCAGTTGTTCACCATTAATAAGCAACTAAGTAAGACAAAAAGCCCCAGAAAGACTATCCCACCCCAAAGGTTGTACTTTTGGAGATAGTGCGTTTTATTAGGATTGTCCAACTGGTAACCCTTGGCTTGTAGCGCAAACGCGACTGCCTCGGTTTTTTTGACCGTTGACAATAACACCGGAATAAAGAGCGTTTTGAGTTTGACTGGATTTAAAAAGGCTAGCTTACCGTACTTAAAACCACGGATTGCTTGCGCTTGTTTAATCCGTTTTAATTCACCCTTAACAATGGGTAACAGTTTAAACACCAGTGCAAATAAGATGCTTAACGGTTGTGTTTTAATTTTGAGGTAGCGCAAAGGGGTACACAACCGTTCGACCCCAAACGCTAACTCATAAATCGAAGTGGACGCAGTTAAGAGAAAGGACGTGGCAAACAGCATGCTAATTCGTAGTGCTATTACCAATGAACGTAGTAAAGAGCGGGTATTGACACTCCACCAACTCACCCCACCAAACTGTTCACCGCCTGAATAAATAAAATGACCTAAAAAATTTTCATTTTGACTGGCCAATGCACTCGAATTCGGTAAGAAAATAAAGCCGTTGACGACAATGTTAAACCAAAAGAAGAATAACAGTCAGCCAAAGATAATGGCAAACGTTTTGAGTTTGCGCTCCACTACAATCACAAGCGCAAAGAAGATCGCATTAATAATAATTAGTCCGTGTAGCCCTAACGGGAGAAAGGCCACCACAAGGCTAATAATTCACCACCACAGCTTTAAAACGGGTGGTAACTGACTTAAAAAGTTTTTACTGGTCATGGAAGAGCGGTTTGAGTTGATCAATCAGATCGTGTACGGTTTTAATGGCTAAGGGATCGTTTAACTGCACACCCTTTGCTTGTAACAACTGAATGGTTTGCACCACAAGTGGTGGGGTAATTTCGTACCGTTGTAAAAGCTGCATGTTGTTAAAGAAATCTGCAACACTCATTTGGTCGACAATTTGACCAGCCTCTAATTGAATCAGACTGTCAGCGGTTTCGTAAATGAAATCAACGTCGTGACTGACAATCATAATGCGCGTGACCTGTTTTAATTGTTTGAGTAAAGCACTTTTAAGTGCTTTACCCTCCTGGTCTAACCCGACCGTTGGTTCGTCCAAAATGAGAAATTGCACTTGCATGGCTAATACACTCGCCAATGCCAAGCGTTTCTTTTGTCCGCCACTCAACTCCAAGGGGTTTTTAATTAGGGGAATGTGGTCCAAACCACACATCTCTAAGTACTTTTTGGCAAAGTTGACATCACCCTTTTGACACAAACCGAGGTTAATCGCTCCCGTTAACACCTCTTCTAAAACGGAATCACAGAAAAACTGTTCTTCGGCTTTTTGTAACACATAGCCAATGCTGTTTTGGAACTGCTTTGTGGCACGGTTATGTTTTTGGTTGGCAAAGTAATAAAAGTTACCACAGTTCAAAAAACCACAGGTCGGATTTTCAACTAAACCTAACTGTTTAATTAGGGTCGTTTTGCCACTGCCACTAGGACCAAAAATAACGGTAATTTGGTTAGGTTCCACACTACCGGTAACGTTTTTAATAATTTGACCAGCATTGCTTTTATCAACAAAGACACTAAGGTTGTAAAAATACAGCGTACTTTGCGCTAAATCAACTAACTGCTGGTTAAACAGCGGGGTGTGTTTTTTAGCTGTTAAATAATAAAAAGGGTGCACCAGTTTATCAAACTGTTTGCAGTACCAAAAGTAGTGTTTAATGCTGCTTTGAATCAGTTTGCGGTTAACCTTATTGGCTAAACCACGGTACTGTGGTGCAATCAGTGATCAAATTATTTGAGGTGTTTGCATATTCAATCAACAACGTCTTGCAACTCCTCAATTGGACCCTTCATTTCGTGGTGGAGATGATCCGCTACTTCATGGGCCAAAAGTCATGGAAACGGGAGGGTAAAGTGGTGCTGGTGGAACAAGTGGAGCTGTTTGTATATGGCAGCGGGTGAAAACTGTTTTAACAGCTGTCCCTTACTTAAAAAGATAATTTCATCCGCTAAGAAGAGGTCATTAAAGTCATGGGTAACATTAATAACTGTTTTTTGCTGTTCTTTAATTACCTTAAAAATGAACCGCTTTAGTTTGCTAGCTAGCTTACCATCCAACATACTAAAGGCTTCATCTAGTAAATAAAGTTCGGGTTTCACCGCCAGTAAACAAGCAAACACGGCCCGTTGTTTTTCGCCAAACGACAGCTTACTGAGCGGTGTGAACTGTTTACCTTGTAGTTCTACAACCGCTAAAACATCAGCAATCACTCGGTCCATTTGTTTAGGATCCACCCCGTGGTTTTCCAAGCTAAAAATGAGCTCTTGGTGCAGCGTATCACCCAACAGTTGCATGTCGGGATCTTGCAGTAAGATACCAATTTGGCGAAAACCAACTTCTTCCAAGGGTTGACCCCTAAAAAAGATTGTTTGTTTCGGTGCTTTTAAAAATCCGCCTAACAGTTTGACCAGTGTCGATTTACCCGAACCATTGTGGCCAATAATGGCTACATGGGTGTTGGGTTTGAGGCTAAAATTGAGCTTTTCTAAGGCCGGTTGACTGTGGCGCGGATAGGTAAAACTGAGGTTGGTAACGTTGAGCAATTTAAGTTTTTATTTTTAAATTTTAAAAATAAGACTAATTGAAAAAATGGTTTAAAAAGTGCTTAAAGACGTCTATAAACCATTATTACAGGTCAAATAAGACATAAAAAAAGAGCAGTTTAACTGCCCTCTTTTTATTGATCTACTGCAACAGTAATTATTGTGCTGGTTGACCTTGATCCCCGTTAGGGTTGTACTTCTTGGCCATTTCTTCCCTTCACTTCTTGAAGTTTTCGATTTCGGCTTTTAACTTGGTGTAGTCATTGGCCTTAATTGCTGCATCAATACCACCGGTAATCTTGTCGAGTTTTTCCTTCTCTTCTTTAGGGAAGTCCTTCGCTTCGGGACTTTGGAGAATCTCCTTAATCGTGCTCACGATGCTTTCACCTTCGTTACGGAGCTCAATGCGTTCACGAATCACATTGTCACGCTCCTTGTTGGCTTCCGCATCACGAATCATCTTTTGGATTTCCTCTTCGGACAAGTTGCCGTTGTCACTAATAGTAATACTGTTTTCCTTTTGAGTGGTTAAATCTTTAGCCTTCACGTTTAAGATCCCGTTGGCGTCCAAGCTAAAGGTAATTTCAATTTGGGGTTTACCCTTGGGTGCTGGTTGGATGCCCCCTAAGTTAAAGCGACCCAAAGACTTGTTGTCACGTGCCATTGGGCGTTCCCCTTGACAAACCACCACATCCACTGATTCTTGGTTGTCTTGCGCTGTAGAGAAGATTTGACTCTTACTTACAGGAATGGTGGTGTTACGCTTAATTAACGGAGTTGCTACACCACCAAGGGTTTCAATCGAGAGCGTTAAAGGAGTAACGTCCAACAGTAACACGTCCTTTACATCACCGCGTAACACACCCCCTTGGATGGCAGCACCAATGGCTACCACCTCATCCGGGTTAATTGAGCGGTTTGGACTGTGTCCTGGTACCATTGATTCCACTAGTTTTTGCACTGCTGGCATCCGGGTCGATCCACCCACCAACAAGATTTCATTAATTTCTTCTGGTTTAATCTTGGCTTCACGTAAAACATCCGAAATTGGGTTGCGAGTACGTTCTAGTAATTGTTTGGTAATTTCTTCAAACTTAGCACGGGTTAGCTTAAGTTCAACGTTTACCGGACCCTTTTCGGTAACCGTTAAGAACGGTAAAGAGATAATTGCTTCTAGTTGGGCGGAAAGTTCAATCTTAGCACGTTCAGCCGCTTCCTTTAAGCGTTGCATAGCCATCTTGTCATTGGACAAGTTAAGCCCATTGTGTTCTTGGGCAATGTGCGCTAAGATGAACTCAATAATCTTGTTGTCCCAGTCATCACCACCCAAACGGTTGTCCCCAGCAGTGGCTAATACTTCGAAGGTACCTTCAGCAATGTCAAGTAAGGAAACGTCAAAGGTACCACCACCAAGGTCGTACACCAAGACTTTCATTTCTCGGTTAGACTTGTCGATCCCATAAGCCAATGCAGCGGCGGTAGGTTCGTTAATAATCCGCTCAACGTTTAAACCAGCAATCTTACCAGCGGTTTTAGTAGCGTTCCGTTCTGCATCGTTAAAGTAAGCAGGTACGGTAATAACAGCACGGGAAATCGTTTTACCAATCTTCTTTTCCGCATAGTCCTTGAGGTAGCTCAAGATTTGCGCTGATACCTCTTCAGGAGTTAACTCTTTGGTAGAACCATCAGGATTCTTAACGGTTACCTTATTGGAGGTACCCATTAAACGCTTAATGGAAACAATAGTATTAGGGTTAGTTACCATTTGCCGTTTCGCAGCATCACCCACAATAATTTCGTTGTTCTTGTAAGAAACAATCGAAGGGGTGGTGCGTTTACCTTCAGGGTTTTCCAACACTACTGGGCGTCCATTCTCCATGACCGACACACAGGAGTTAGTGGTACCAAGGTCAATGCCGATAATTAAGCCGTTATCTGTACTCATATGTTTATGTGCTAAATATTATAAACAAATCGTTAGCACTTTTAGCGTTTGAGTGCTAATTATGTAACTTTTTATTGTTCCAAAAGTGGTGGTTAATCAAGTTAACTGTTGGTAATACTGGTTTGGGGTGTTTGAATTCAATTACTACTTTAACCACTCAACCAATAAAACAGAGACTAAATAAGATAGCTGGAATATAAACAGAGAGAAACCTTCACAAAAAGACCCCTTGCTTTACTTGCGATTCAATACCAACAGCCGATTTAAAGGCATTTAAAAAGCTTGTCATTACAAACTGGGTAGCACCTTCCCCACTAGGTACGGGAATAAAGTTAGAAGCGGTAATGGCAATGTTAGTGATGTTGAAAATGTCAGCCGTAGAGTACTGGTCAATAACGTTGTTAGTTGCGTTTTCCTTTTTGACAATGGCAAAAACAGCAAACACACTAAAGTAACCCACCACTGCTATTAAGATATTGGCTAGTAATTTGAAGATCGTCAAGCCTAAACGTTTAATTTCTAAACCATACAACTTATTAAACTCTGCCTTATCAATAAAACGCTGGTAAATCTGTTCCTTAGTTAAGTAAGGACGCTTAATTCACTTACGAAACTGGTTTACCCCGGAGTAAATTAAGACATGCATCCTTTTACTGTAGGCAATAAAGATAAATAAGATGGCTACCACCACATCAAAGATCATCCCCGCAAAACTAAATCAGTACGACACAAACCCTTCGTGGTTTTGTTCTAACAAAGCATAGTTTTGGGAAAGAACAAAGAAAGATGGTCAAGTAATTAACGCTTGGGCAAGGTTCCAAAAGGCGCCCGTGGAAGTAACTAATAAGACCGCTGTCTGCTTCTTCATGCCCTTTTTAATGAACCAGTACAAGCGAAAGGGATCTTGACCCAAGGAAAGTGGGGTGACAATTTGAAAGAACTGCACTACACAAGCAAACAGAACCCACTCCCATCAAGGGGCATGGAAGCAAAAGCGGCGTGCTACTCATCAATTAATGATTATGTTTCAAGCTAGGGTAACCACAATCCCCAATACTATTAACAGTATTCAGGGTCAGTTAGTTCTGTTAATGGTGTTAATAATCGTTTTAACATCACCAGTTTTAATGTCTAAAAAGAAGACGGTCACCAATACACTAACAACCACCAAAAAGGCAATAAAAAAGCTAAAGGCAAGAATATTTTTTCTAGTAAAAAAACTAGCGCTTTTAGGGGTTGACATTGACCTTTGCTATTTGAGCAATTTTAACCCACTTTCTATCCGTTTATCACCAATATTTAACTGCGGCTTATTAATGGCACTAAGTGCCAACTGTTGTGTGCCCTTGTCTGCTGCTTGGAATAAAAGCAGCGCTGCCACAACATCACTGGCAATTTTATTGTTGGTACCGCTTGTAGTTGTTAGACTCAAGCTGTCAATGTCTTGGTAAGTGAACTGGGTGTAGGTGTACTTGTCACTACCATTTTGGTAAGTGAGGTAGTTGCCAGTTCCACTTGGAGTAGAATCCCTTTGGCTCTTTTGGGCACCATTACCAAAGTAGTTTTGGTACTGGTCCAAGAGGTCTAATAACAGTTGACGCTTAGCTTGGAAGTCCAAAGTTGGGTTGCCAACGGCATCCACTTGGAAGTAGGTCGCAATTGTTTTGCCACTGCCACCATTAGTTGTGGAAGGATTTAATCCACTTAACTGGACTGCAATCCGGTCTAGTAACAATTGGTTATTAGTCCCTTGAATGATCTTTTTGAGTTGATCCTTGGAACCGTACTGTTGCAAGGAGGTTTTCGTAATTTGCTGCAAAATGGCATCACGGGAACTAGCATCGAACATCGAACTAGTGCTGGTACTCAAACCAGTAAAACCATAGTGATCAGTTGAATTTTGTTGCATTTGTTGTTTTTGTACTAATGCTAAGCTAGCAACTTGTTGAGTGGCTTGTACCGTTGGCTTGGTTGTTGTTTTGTAAAAGAAGTTATTGGGATCTAGTGTTTGAGTGGATTCTTTAACTTCGTTAGCGGAGTTCTCATAGTTGAAGTAGGAACCAAATACGCCTTGGGGATTAGAGAAGTTAGTGGTTTCAGTGTTCTTGTAAGCTATAAAAGCAACTTCACCAAACTTAAGGTTTGTTTTTAACAGTGACTTAAAGTTCTTTAATTTTTGGTCCACTAGCCACTGTAAGGTATAGATAAAACGGTAATGGTCAATGACCGCTTTGTAATCAACGTTAAAGGACTTTTCGTCGATGCTAGCTTCAAAAGCCAGTTTGAAAAGCTCATCAAACTTGTTGTCCTTCGGGTTACCGTAAATCGATTTGTCCGTGAGTTTAGCCCAAGTAGTGTAGTAATAGTTACCGATAACTTTACTTAATGCATCGGCATCACTGTTGGTTGTGGAACTACTTTGCATTCCACCATTATTACTGGACTTTTCCTGAATGTCTTTAAACAACTTTTGTGCTTTCTTAAATAAATCGTTATTATCGAACGCTTGTTTGAGCAACATAAACGAAAGCGGGTTGACTTTGTCGCTCTGTAATAAGCTAGCTAAGCTCCAGTTAGCACCACTAGAAAGTACTTTGTCATTGTTAACGTTTAACAAAATGACGTTACTGTAAGCTGGTCGGGGTGCTAAAGCAGGTTTGAGTTTGTTTTTAACAAAATCATTTACTTTAGTTTGGATGTTTTCCAAGATTTTGTCCCTAATCTTGTCAACGTCAGCAGTTTTAGTTTTGAGCTTATTGCTTTCTTCACTTATGCTTGTTTCTTCTTGACCAACTTTAGGTCACAATGTTTTTGTGATTAAGAAGGTAAAGTAGTTGTTTAACTTTTCGTAATTACCGTTAGTATTAACGTAAGGTAACTTGGCTGACAAACCGTTCAACCAAGGGGTTGGATTGGCTTGGTTGACGTTTTGGTTAAGATTACGGGCGCGCTCTAGCAACTTGTTATTGAGCGCTTTCATTTGATCAAAATAAAAGCTGATCATTGTGGTTGCTAAAGCCACCGGCGCAAATTCTTTAATTAACTCTTTATAAGGATTAAAAGCTTCAGTTAAAGCTTTGCTAGCTTTATCCTTTTGGTCAAGACTAGCGTTAACTAAGGCGTTGGCCAAAATTAGTTCCCGGTGTTTATCAAACCAACTTTTTAGCGTTTCGTTTAAGGGAAAACTAAAGGCAAAGTTAGTACTGTAGAAAGTGTTAGTTTTAGCTTGAAAGGCACGAAACTTTAAGAATTGTTTTTGCTTGTCAAGGTCTCTTAATACTTTATTACTGTTACCTGAACTAGATTTAGTTAATAAGGTTAAACTTTGCTTAGAAACTGATTTTTTATTTGTTGAATCTTGTTTTAAATAATAGCTCCCACCATCAAGGCCGAAAACAGTTACTCCGGTGCTATTACGCACAATCCGGAATTTAATGGGACTGTAACTGTTTTGATCAGTTTGCTGAACCTGCCTTAAATTTATAGATCCAGATTTTAAGGCAACTAAAACTTCATTAGAAGCGCTATTATTTTTCGATTTTGATGTTGTTGAATTAAATGTAGGAATAGCTGCATCTAAAACAACGTAATTGTTTTGTTGGCCCTTAGAATTGTTAGTTCTTAAAACAGCTGTTTTCGCTTCCATTTTTGTTGCAGCTGCTTTCTTTACCTGCTCCGCTAACTTACTTAAGTTAGTGCCTTCGCTTGTGTTTTGAGCAAAAATGTCATCTAAAGCGCCAGCAATTGGATCTGAGTCACTTTGTTGTTGCTTACCTTCTTGATCACTGTAAAACAGTGCGTTAGTTTTGGAAGCTTTTTCTACGTCCTTACTTTTATCGACAACAATCGTGTTGTTAAGGTCTTCTTTGAGCTCCTTAATTTGCGGCAATTCGTCATTATCTTGCTCAAAAACTTGCAGTTTTAAATTTAAAGCTGCACCTAAGTTAGCCGCTTCTAACTGATCGACAATGTTAGAAGCGTCTACTAATTGCTTCGTTTCGTTAGTATCTGATAAATTTAAGGGAAAATCAAGAAAATCACCCTTATTTGATTCATTACGGTATTTGAGCAGACCAGTTTTTTGGCTGTTTGTACCGCTTTGACCGATATTATTATCAGTAATAGCGCCCTCACCTTTAACTAACTGCTTTCAGCGCTTATTACCAACGTTTTGGTTGGGTGCAATGTCTTTTTCTTCGGCAAAGAAAGGAAAGGCATAAGAAGGTGTTAGTTTATCCTTTAACTTCTCTCTGTTATAGATCTGACCTAACCCTTGACTGGGAGCAGAATACTTATAGTTAACTTGACTAATTAAAGTGGGATCAGTGTACTCCACCCATTGGTCAAATACTTGGCTTTGAATTTTGGCGAAAAAGTCATCATTAGTTTCACTAAAACGTGGGGCACTAAATTCAAAGTTTTTTCAGCTTTCTTCCTTGTGTAGTTCATCATAAGTAAATGGACCAGTAGAAACTTGTCCATCTTTTTTGTAATTGAGGTAGTTCTTTTGGAAAACCCGACTAGCAAAGTCACTAATGAGCTGTTCAAGCAGCTTTTGCGCTTTCCAGGTTGCTTCACTACCACCGGCGTTATCCAACAAATCTTGTTGCACAAAAAGTGATTTATTGCGTGATGCTGATACCACTTTATCGACTGCCGTTGTGTACTGACTGTCAACATTGGACTTAGTGTCTTTTAAAAACTGGGTAATCTTTTTGTCTTCGTTGTTTTCGTACCAAGCTTCTAGTGCTTTTAAAAGTGGTGCAGCGATAAATTGTTTGTTGGCCACTGGATTTTTTAAAGCCGTTGTTAGGGCACTTTGTAAACTACCCTGTTCACCATTGAAAAACTGACTGGAACGCGCTTTAAAAACGGTTTGTAAAGCCGAGTTGGAAGGGGTAGCACAAGCAGCTAAAATAACACTAGTAGACAGAAAACCGCCTAATGTTAAGAGTCAAAATTGGGGTTTTCTTAGAAACTTTTTCATTAACGCGGTTTTTGCTCTCTAAAGGTTAAATTTTTGCTTTATTTTGGCACTTTAAAAGCTAGTTTTTACCTAAAAATAAGTATTGATAAGTAAGAAAAACTGCTTTAGTGCCCACTGGTGCAACTAGCTTAATCTCTTGGCTTAATTTTGTACATTTTAAACCACGATGGCATGACAAACTTACAATTTCAAACGTTTTTTTTTTTTGAAAGAAATTGATTGCTTTAACTAACCTGCTTTTATCTAACTATTTAAGTAAATCTATTCCTGTTTTAATCCGCTTATCACCAATTGGTAGTTGTGGTTTATTAATAGCACTCAAGGCTAATTGTTGGGTACCTTTATCCGCTGCTTGGAAAAGTAAAAGGGCAGCCACAACATCGCTAGCAAGCTTACTATTTCCTTTATCACTCAAACTTAAGTTATCTATATCTTTATAGGAGAACTGAAGGTAAGTGATCTTGTCACTGCCATCTTGGTAAGTTGAATAGCTTCCATGTCCACCTTTACCAGAATCTCTTTGTGCAGCCTGAGTATTTGTACCAAAGAAATCTTTGTACTGATCTAAAACATCTAATAAAAGTTTTCGTTTTTGTTGAAAGCTAAGGGTTGGGTTGCCAATGGCATCTAATTGGAAATAAGTAGCAATATTTTTACCCTTTCCATTATTACTATTTCCAGTGGTTGAAGGATTTAATCCACTTAATTGACTAGCGATTCGGTCTAATAAAAGTTGGTTATTAGTCTTTTCAATCAAAGTTTTCAGTTCTTGCTGCGAACCATATTGTTGCAAGGAATTCTTGGTTATTTGATCCAAAATGGCTTGCTGCGTAGCGGCACCTAACATTGAACTAGTACTGGTGTTTAGCCCGGTAAAACCATAATGATTACTATCTTGCATGGTACTTTGCATTTTTTGTTGCATAACCATCATGCTTGCACCATTTTGTGCGGTTTTAACTTCTTCTGGTTTGGTAGTTGTTTTATAAAAGAAATTATTTGGGTCTAAGTTTTGCGCAGCAATCTTAACTTCATTAGTTTCGTTTTCGTAATTAAAAGCAGAACCAAATACACCTTGCGGATTGGAAAATTCTTTGGTTTCAGTGTTTTTATAAGCAATGAAAGCAATTTCACCAAACTTTAAATTTGCTTTTAATAAGTCCTTAAAGTTCTTTAAGTTACCGTTAACTAGTCATTCCAAGGTATAGATAAAGCGGTAGTGTTCAATGACCGCTTTGTAATCAACATTAAAGGACTTTTCGTTAATACTATCTTCAAAAGCTAATTTAAATAAGTCATCAAATTTGTTGTCCTTAGGATTTCCATAAATGGATTTATCAGTCAATTTAGCTCAACTGTTGTAGTAATAGTTGCCAATTACTTTACTCAAGGCATCAGCTCCTTCACTACTCGAGGTATTGCTACCTTGCATCCCACCTGAACTTCCACCATTTGTTTTACTTTGAATATCTTCAAAAAGTTTCTTAGCTTTCTTAAACAAGTCGTTTTGGTCAAACGTTTGTTTGAGCAACATAAATGAAAGTGGATTAATTTTGCTCGTATCCAATAAGTTAGCTAAAGCTCAATTGGCGCCCATCGATCAAACCTTATCGTTGTTAACGTTTAACAAAATTACATTACTGTAAGTTTGTCGTGGTGCTAAGGTTGGTTTAAGCTTGTTTTTAACAAATTCTTGAACCTTCTTTTGAATGTCTTCTAAGATTTTATCTCTAATTTTGTTAACGTCTTCGGTCTTAGTTTTGAGCTTGTTAGTGCTACTATCTTTAGATAATGAAGCGTCTTCATTACCGACCTTCTTCCACATGTATTTAACAATTAAGAAAGTGAAGTAATTGTTGAGTTTTTCGTAATTACCATTTGTTCTAACATAAGGCAATTTAGCTGATAAACCATTTAACCAAGGTGTAGGATTGGCCTGATTCACATTTTGGTTTAATGCTTTAGCGCGCTCCAAAAACTTATTGAACGCGTTCATTTGGTTAAACAAGAAGTTAATCATGGTTGTGGCCAAACCAATTGGTGCATATTCTGCCAGTAAATCCTTGTAAGGCTTTAAGGCTTCAGTTAAAGCTTTGTTTGCTTTATCCTTTTGATCAAAATTTGCCTTTACCAAAGCGTTCGCTAAGATTAGTTCGCGGTGTTTATCAAATCAACCTTTCAAGGTTTCATTTAACGGAAACGAAAAGGCATATTTGGTGCCGCCTTGTTTGAAAACTCTATTTTTAGCTTGAAAGGCACGAAACTTCAATAAATTTTTTTGAAGACTGCTATTAATCTCTTGTTCTTGATTATTATTACTTTGGTTTTGAGCGAGAGAAGTGGCTATCGTAGAAGAGCTACTTTGTTGTTTATTGGAAGATCCAACAACGTAGTAAAGCAAGCCTTCAAGAGCCAGCAATTTTACACCAGTACTATTTCGAACAATGCGCTGGTTTAAAGGCGTTGTACTACTTTTTTGTTGAGTTAAATTGACGCTAGTAGAAGTCAAATTAACTAAAGCGCCACTACCAGAAATAGTTCCACTGCTTTGTGGTGATTGTGAAGAAGTTTCAAACGCCGGGATCGCAGCATCTAATAACACATATTTTTCATCAGATTGTGCCATGTTTGTGGTTTTGAGACTAACCGCATTTCTTAGTTGTCATGTCTGACTTCTAGCTTCCATTTTAGTACTGTCAGCTTTTTGTAACTGTTCAGCTAGCTTACCTAAATTACCACTATCGCCATTTTGACCATTAAAAATTTCCTTTAATTGAGTTTTAGGTGAATTTTTGCTTTCAGCACTTCCTTGTTTTTCGCTACTATCACCATTATCTTTGTAAAAAGTACTGTTAGTTTTAGAAGTTTTCTCTAAGTTATCAGATTTAGCTACAATGGTGTCATTCAAATTGTCAGGAAGCTTTTTAATAACAGGCAGTTCATCAAAGTCCTGTTTTAAAAAACTGAGTTTAAGGTTAAGTGCTGCTCCTAAATCGGCTGCTTCTAGTTGATCAACAATAGTAGTGGCATCAACTATTTGTTTAGTTTCACCAACATCAGTAAGAGTATTCGGGAAATCAAATAAGTTTCCGCTTGATGTATCACCTCTTCATCCAAATAACCCTTTTGTAGAAGAGGAACTATCGTCGTCATCTGAACTCTTCTCCTTGCCATCATCAGAGCCCTCGCCTTTAATTAATTTCTTTCAGCGCTTGTTACCAACGTTTTGATTTTCCGGAATATCTTTTTCCTCAGCAAAAAAAGGGGAAGGCATAGGAAGGAGTAAGTTTATCCTTTAACTTATCTTTGTTATAAATTTGACCTAAACCTTGATTAGGAGCTGAATACTTTAAGGTAACTGAGCTAATAATAGTTGGATCGGTGTACTCAGCCCATTGATCAAAAACTTCGCTTTGAATCTGCGCGAAAAAATCATCATTAGTTTCTGGAAAATTACCAGCATCAAAAACTATATTCTGTCAATTACTATTTTTTGAAATCGTGTCGTATAAAAACGGACCGGCTGATACTTTGCCATTTTCCTTATAAGACAAATAATTCTTTTGAAAAACTCGTGAAGCAAAATCACTAATTAGCTGTTCAAACAGTTTGCGCGCTTTTCAAGTGGCCTCACTACCACCGCTACTGTCCAATAAATCTTGTTGTACAAATAAGGATTTATTGCGTGGTGCTGAAACTACTTTATCTACAACGGTTTTGTATTGGTTATCAACATTAGTCTTGGTGTCTTTTAAGAATTGAGTAATGTTTTTATCTTGATTATTCTCGTACCAAGCTTCTAATGCTTTTAAAAGTGGAGCAGCTACAAATTTTTTGTTAGTTTCCGGATCTCTTAAAGCGGTGTTTAAAGCGCTTTGAATGGTACCATGCTCACCGTTAAAAAATTGGTTGGAAGTTGGTTTAAAAACTGCTTGCAGTGCTGAGTTTGAAGGTGTTGCACAGGCACTGAGAATAATACTACTAGTGGAAAGGCCACCCAACAATAAAAGTCAAAACTTGGGTTTTCTTAAAAAACTCTTCATTTAAAATAGTGCCGTTTGTTCTAAAAAAGAAAAAAGCAAGCTTACTTTTTGGCTTAAGGTTTTTAAATTTTAAAGCACCTCGATATTTTTGAAAAATTATTTCTAAACTTTTTTTTTTTTTGCAAAAAGTTGTTAGGCTTAAAACCAATCTTTTAGCTCACTATTTCAGTAAATCGATCCCTGTTTTTATGCGTTTATCGCCAATTGGTAATTGTGGTTTATTGATGGAACTTAAAGCCAGTTGTTGAGTACCTTTATCGGCTGCCTGGAATAATAAAAGCGCTGCTACAACATCACTGGCAAATTTGCTGCTAGTTCCGTTTGAACTACTCAAACTTAAACCGTCAATATCTTTATAGGAAAACTGCAAATAAGTGATCTTATCACTACCGTCGGTATAGGTTAAATAGTTGCCAGTTCCACTCTTACCAGAATCTCTTTGAACTGCTTGTGCATTATTACCAAAGAAATCTTTGTATTGATCTAAAACATCCAATAAGAGTTTTTGCTTCGCTTTAAAGTCCAAAGTAGGATTGCCAACCGCATCTGTTTGGAAGTATGCAGCAATTGTTTTTTGGGTACCACTGTTGCCACTTGTATTCGGTTTTAAGGCTATTAGTTGGTTGGCAATTCGGTCTAATAATAATTGATTTTTAGTTTCGCCAATGATCTTTTTCAGGTCAGCTTGAGAACCATACTGTTGCAAGGAAGTTTTGGTTATTTGATCCAAAATGGCTTGCTGGGTAGCAGCACCTAACATCGAACCACTACTGGTACTCAAACCAGTAAAGCCATAACTGTTAGTTTGCTGATTGTTCATCTGCGTATTTTGAACCATCACAGCTGTATTAGCACTTTGAGTGGTTTTCGCTTCTGGTTTAGTGGTGGTTTTATAGAAGAAACTGTTGGGGTCTATAATTTGCGTAGCACTCTTAGCCGCATTCGTTTCGTTTTCGTAATTAAAGTAAGAACCGAATATACCTTGCGGGTTAGAGAAGTTCTGCGTTTCAGTATTCTTGTAAGCAATAAAAGCAATTTCACCAAACTTGAGGTTTGCCTTTAATAAATCCTTGAAGTTCTTTAGGTTTTTGTCTACCAACCACTCTAAGGTATAGATAAAGCGGTAGTGTTCAATCACTGCCTTATAATCTACATTAAAGGACTTTTCGTTAATACTACTGTCAAAAGCCAATTTAAAGAGATCATCAAATTTGTCATCCTTAGGGTTGCCATAAATTGATTTATCGGTTAGCTTAGCTCAAGTGTTGTAGTAATAGTTGCCAATGACTTTGCTCAAAGCATCGGCATCATCGTTGGTGGTTGTACCACTTTTACCACTTCCACTAGTTTTTGCTTTATTTTGAATGTTTTCGAATAAAGTCTTTGCTTTTTTAAACAAACTGTTTTGATCAAACATTTGTTTGAGCAGCATAAACGAAAGCGGGTTAACCTTTTTCGGATCTAGTAAAACTGCCAAACTTCAATTAGCGCCAGCAGACCACACCTTATCATTGTTAATGTTTAACAAGATTACGTTACTGTAAGCTGGTCGGGGTGCTAAGGTAGGTTTGAGTTTTTGGACAAAAGTTTTGAGCTTACTGTCGATGTTGTTCAAAATCTTTTCCCTAATCTTGTTAACATCTTCAGTTTTAGTTTTGAGTTTGCTTTTTTCTTCACTGACATTAGTATCCTCAGTGCCCACCTTAGGTCACAAAACTTTGGTAATTAAAAAGGTAAAGTAGTTGTTTAACTTTTCGTAATTACCGTTAGTGTTAACGTAAGGTAGTTTAGCCGATAACCCATTTAACCAAGGGGTAGGATTGGCTTGGTTAACATTTTGGTTTAAGCTCCGTACTCGTTCTAACAGCTTATTGTTGAGAGCTTTCATTTGGTCAAAATAAAAGCTAATCATGGTGGTAGCTAAACCAATCGGGGTGTATTCACTCAATCAGTTTTTATAAGAACTAAAAGCATCCACTAAGGCCTTACTTGCTTTTTCCTTTGTGTCTAAAGTAACTTTAACTAAGGCGTTCGCTAAGATCAATCCGCGGTGTTTGTCAAACCAACCTTTTAAAGTTTCGTTTAGGGGAAAACTAAAGGCAAAGTTAGTGTTATAAAATGTGCCAATTTTGGCTTGAAAAGCACGGAACTTTAAGAATTTCTTTTGCTCTTCTTTATCAATCTTTTTGGCACTACTAGAACCGCCATTGCTACCTCCGCTACCGCCTTTTATGAATTTAACAAAACCATCAAGTCCTAAAACTGATACGCCAACACTGTTACGCACAAGTCTCAGGCCAAAAGTGTTATATTGATCTTTAGTTTCTCCTTGCTGTTGCATTTTTAGTTAGGCAACTGTAGATGTGTTTAAAGAAATTAAGGTACCGTTATGAAGACTCACTGAACCTTGACCCTGGGTGCTGACTTGCTGTTTAAAGGTAGGAATACCAGCATCTAATAGAACATAACTGTTCTTTTGTTGAGTTTGCGATTTATTTCGCAAAACAACAGTGCCTCTTGCTTGTGGGGTTTGCGTTTTAATTTCCGCTGTCTTTTTAATTTGTTCTGCTAACTTGCCTAGATTAGTTTTTTGTTCCACGTTTGTGGCAAAGATGTCATCTAAAGCTTGGGCAATGGGATCGGTATTTGTGGTTCCAGAGTTTTGATTATCCTCATAAAAAGAAGAATTAGTTTTGCTCACATTTTGTGCATCACTACTCTTATCAATCACAATCACGTTGTTAAGGTCATCTTTGAGCTCCTTAAATTCGAGTAGCTCATCCTTCTCTTGACCCAGTCGGTCTAGTTTAATGTTGAGCACAGCGGCTAAATCAGCTGCCTCTAATTGATCGACAATGTTGGTGGCATCCACGACTTTTTTGGTCTCGTTAGTATCTGATAAGCTTAACGGAAAGTCAATTACTTGGCCTCTGTCGGTTCCCCGTACATACTTGGTAAGGCCTTCTTTTTGAACTCCTTTTGTTTGACTGCCATCTTGTTGTTGCTTTGGCTTTTCTTCAATCGCTTCTTCACCTTTAACCAGTTGTTTTCAGCGTTTATTGCCGACATTTTGACTCCCAGCAATTTCATCCGCTGCAGCAAAAAACGGAAAGGCATAGGAAGGGGTTAATTTATCCTTTAGTTTGTCCTGGTTATAGATTTGCCCTAATCCCTGGTTAGGAGCCGAGTACTTTAAGGTAGTGAAACTAATAACAGTGGGATCGGTGTATTCCGCCCATTGATCGAAAACTTCGCTTTGAATCTGGGCAAAGAAGGCATCATTGATTTCGGGAAACTGGCCCGCATCAAAGACAATTTTGTCCCAATTGCTCGATTTAGAAATTTCGTCAAATAAGAAAGGACCTGATGATACCTTATTACCATCCTTATAAGCGAGGTAGTTCTTTTGAAAGACCCGACTAGCAAAATCGCTAATGAGCTGTTCAAACAACTTCTGGGCTTTTCAAGTTTCTAACCCCCCCGGCGCTATCCAGCAAGTCTTGTTGGACAAAGAGGTTTTTGTTTCGGCTAGCCGATACCACCTTATCTACGGCTTGGTTGTATTGCTCATCAACACTCTTTTTGGTGTCTTTAAAGAACTGGGTGACCTGTTTGTCTTGGTTATTTTCATACCAAGCTGTCAATGCCTTTAAAAGGGGAGCAGCGACAAACTGTTTGTTGGCCTCTGGATCTTTTAAAGCAGTGGCTAAAGCGTTTTGTAAACTACCCTGTTCCCCGTTGAAGAACTGGTTCGAACGTGCTTTAAAAACAGTTTGGAGTGCTGAATTGGAAGGGGTAGCACAGGCAACTAAAATAACACTTGAAGAGATAAAGCCACCAAGGGTTAAGAATCAAAACTGGGGTTTTATTAGAAGTTTTTTCATGAACGGCGTTGTTTGCGCTTGGCAAAGAGTTCCGCAATCTGCTGCTTTTGGCGCTTCTTATAGTTAGGCTTGACCTTCTTGTATCCTGCCTTAATCCGCTTAATGGCAGTTTTAAGTTCACTGGATACTGCTGGTTTTTGTTTGCTTTGTTTAAGTTGGGGGAACTTTAAAGTACCATCACGTTCTAAATGAACACTGTTAAACAAAATACCTTTATGGGCAAACTTAGTTAATTTGTGTTGGTTATGCGGTAAATCAAACACATAGGAATTGCCCCAACTGTTAAAACGCGCTACTCTACCAGAACGGTGGATGTAAAAGCTATCCACTAGGGGTAAGTCTCATGAAATGACCACACTAAAGTGGGGCAGATCAATGCCGCGTGCTAGTAAGTCAGATACCACTAGTACCCTCACCGTGTTATTAGCGGCACTAGTAAAGATGTGTTTGCGCTCATTGGGCGTTAGTTGACCATGGAGGCTTTTAAAGCTAACTTGGTTGTGGGCTAGTAAACTAGTGAGTTGTTTGAGTGATTTAGCGTTGGAACAAAAGACCAAGACCTGTTCGTTATGGTGGTAGTTGAGTAAAGCTAATAAACCAGCAAACGGTTCTTGACCGTTGAGGTTAAGCAGAAAGTGTTTCACCAAAGAGTGCTTTCAAGCACGTTTCTTTTGGTTGACGACCGTTAAAGGTGCGACTTGCTTGCGAATTTGCTGGAGCTGTTCGTTAAATAACGTGGCACTAAAAAAGCCAAAGTGAGGTTTATGTTTTTGAAACAGTTGGACACAGTTAATCCACTCTGCTTGGGAACTGTCCTCCAAAAACATGTCGACCTCATCAAAGACAACGTACTTAATCGTTTCGTGGAGAATAATGTCTTTGCCAATTAAGCTCACAATCAGGTGGGCATTACTGTTGAAGTTCTCACTGACCTTTAAACCAGTAAAGCTGGCTTTAATGTCGTTGAGGATCGTTAAAATTTGCCACTTCAACTCCTTAGTTGGTACAAACACCACCGCTTGGGGCTTGTTGAGCGTGACATCAATTTGGTTGAGCGCTGGTAAGAGGTAAGCGAATGTTTTACCACTACCAGTTTCGGCTATGCCAACCACATTTTGACGTGGTCAAACTTTAAACACAGCTTGTTGAATAGATGAAAATTCAACAATGTGCTTCGTTTGTAAGAACGCTTGGAGTGGTTGAATGAAGTGCATAGTGGTTTAAAAACGTCTTACCCATTGCGCACTAATAGAACTAAAGATCCTAAAGTCACCTACTTTAACTAAACCCTTCTTATTACCACTAATTAAGGCGTTAATGGCTTGGGTTTGATTATTAGGATCCAAGGCTTCTAGCGCAATTGCAGCGAGGAATTCTTCGAGGGAGAGGTTGAGGCGTTTGTCTTTGCTGGAATCTTCTTGCTTTAGAGAATCAGTGCCGATCTTCTCCACATCATCAAGGTTGACCTGTTTGACAAAGGTAGCGTGGTATTTGCCACCGTCACTTAAGAATTGGTAGGAAGTGTAGTTCTTTTCCTCTACCTTGTTATCACCAATGTAACCATCAAAGCGGCTAAAGACGTCTTTATAGTTGGTATTTTGGTTATCTTCTTGTGTTTTCTTAATTTGTACTGTTGCAGATCTTGCACTGCGTGCACTTGTATCGCCTCTCTGTGCAGATTGAGTACTCTGCTTAAAGTTAGAATCAACAAAACTCGTTAAACTAGTTTTCTGTTCCGTTAAAGTTTTGTTTTTATCAACCCCAGTAACATCAATGTTGAGGTCACTAGTTAAGTGGTTGTAAAGCGCATCGAACTCGGTTTGCGTTTGGATTCCGTTGATGATGTTCTTTAAGTTATCCATCGAACCAAAGCTAAACAAGGCACCTTTGTAAGCGTTGTTGGTGGTGGATTCGGCGTCGTCAGCTTGTTGTTTTAAAACTAAGGTAGCTTTACCACTTTTAGCTGTGGTAATTAGCATTTGACCACCAACTTTAACGCCTTTTTCTGTTTGGTTAACAAACTGCTTAAACAAACGTCTACTTACCGCTTCGGGTAAACTACCACTAGTGCTACTCGTTACAATACCCCTAAAGCCATAGCGGTTTTTGGCAGTTTTTGTGGTCATGCCACTAGCTTGATTTAAACTCCTTAAAACAGTGGCACTACTTTGTGCTGCGCCAGTACTACTACTAGACTTAGTGTAGAAGAAGCTTGAAGTGTCATCAGCAGCCCGGTTCACTTCTGGGGTTTTACTGTTGGTTCAATCATACACACTCGAACCAAACACAAAGTTCGGGTTTTTGACTTCGGTTTGGGTGATGTCACTATTATTATCACCGCCTTGGCCAGCTGCTTGATTTTGTGCTAGGGAAACGACTGATCTTAACTGAATTAAGCCAGTATTACTTGTGCTTGAAGCGGTACTGGATAAAGTTGATTGTGAATCATTTTTATTCTTTTCGCTATCCACACTTCAAACTAAAAAGGCATTGGAAGTACGCGACAACTTCGACTGTAAGCTTTGCTTATAGTTCTTTAAGTTGTCCTTTAACAACCAATGGGCCGTGTAGAGGTAAGACAGATAATCAATTACATCATCACTATCTAAGCGCTCTTCTAAGTTTAGCGAGCTGTACAGTGTGTCCAAGACACCTTCGATGCCCGTCTTAGTTGAAGAATTTTTATCAGATGAACCAACATGACCAAAGATTGATTTTTGGGCTTGTGATTCAAATAAGTCCAATAAATAGGTTTCTTTAAAAATCTTTCAGTTGTCAGCGGTGAGGTTGTTGTTACCGTTGGTGCCACCAGTACCACCACTTTGTTGACTAAAAGTTAAATTCGTAATATCAATAATCTTTTTAAACGCTTCATCTTCCTGGAAGTAACTGAGCTTAATGCTGTTTTGGAGCTCAGTACTGGTTAAATAGTTGTTTAGAGCTAAGTTAAGTCCTAAAGAGGTACTTTCAGGGGTAAAAGACAACTTAGTTTGCACTAAGATTACCTGGGAGTAGAGTGGGGAAGGAATGGTGAAGACTTTGAGTGCCTCTACATGTTTTTTAGCAGCTTCTTCTACCTTCTTCTTATTATCTTTAAGCTTGCTAACTACTTCTTCAGAGAAGTTTTTAGTTTGTCCTGTATTGGAAGAGGTTGTTTTAACAGTACTAGTTCCTTTTTTATCAACATTATCAATAATGTCCTTGTAATAGTTACCAAGGTCATTATAGTGACCGGTTTTAGCATCTTGTTCGTAAGGTAACTTAGCTGCCAAACCGTTCTGTTCAATCTGGTTATTGCGCTCGTTGTCAATAAAAGGTTGGTTACGTTCTGCTAGCTTTAAGGTAACCTTGTTTTCCGCCACTGCAGTTTCGTTAAAGACTGCTTGTTTATAGTGGGCTTGAACAAGTTCTTTTAAATCCTTCTCAATTGACTTAATACTGTCAGCAAACTTCTTAAACTGGGGATAGCTTAAGAAGTTGTCTTTGTTGTTTGTATCACTAGAAAGATCTAATAAAGCTTCAAATAAGAATAAGATCCGGTTAGTGTCAAAGTACTTTTTGACCTCATCAAATAACTTAAAGTCGTATGTGGTGTCGGTATCCACTAAACCGTACTTGGTTTGCAAAGCCCGGAACAATAAGAATTCCTTTTGTTTAGCGACATCACGACCACTTTCAGTAAGATAATGACTACCACCATCCACGGCCATTAAGTGGATGCCGTCTTTACCACGGGAGAGGATGTAGTCTGGTTGACTACTAGAATCTTTTTTAACTTCAATTAAGTCAACAACCGCCTTACTACTGTTGCCAGTAGTAGTTTCAGTAGAAGATCCGGTGCTACTGCCGGTAGAGGTGCCACCGCCGCCTCCAGAACCTCCGCCACCTGTTTGATTAGCATTAGTATTACCTATTTGTTGTTTATACTCACCAGCAAAGACATCTTTATCACCAAAGATTTTGACTAAGTCAGTTTTGTGGACGGTACTTTGTGTAGTTCCACCACCATCACTGGCAGTTTTTAATTGAATAGTTCCTTCAGATGATGAACTCGCAGCCCTTGCAGCGGCGGCTGCCTGTGTATTATTGTCAACAAAAAAGTTTTCAATAATACTCTTATCCTTAATGAGACTGTCAACTTCTTTACTATCAGCGCCCTTCGACTTTTTCTGCAGTGCTAAATAGCCCTGAATGAAAGCAGCAGAAAAACTCGGATCAAAGGTGTCGAACATGTCGCTCGCCTTTAACAACAATTTGCCACCACTATCACTGGAAAACTTATTGGGAATATCAATCCCTTTGCTCTGGTCGTTTTTGTAACTCTTGAGGTTGCTAGCCAATAAGTTAAAGCCATTCGCTCCCTTAGTGCCACCACTCTGTTGGTTGTATTTGTTAAAGACCTGAAACTGGTAGGAAGGGGTTGGACTTTGAATTACTTTAGTGTTAAAGTAATTATCAAACCCGTCAACGGGAGTTTCATTGGTAAACACGACCCGAGATACCAAGTTGGGGTTTTCATTGGTAATAAACTGGTCTAACAGTTTGTCTTGGATGGCCGCGTACACCGCATCATTGTTAATCTTGAGCTTAGCATTCTTATTAACAAATTTGGGTTGGAGCTTAATGTTTTTAAAGTTAGAGGTGTTTTCAATTAAGCTCTTGGTGGGTTTAGTTAAAGGACCTACACCGTCCTGGACATACTCGACAAAGTTCTTGGCAAAGATCCGTGAGATAAAGTCATCAATTATCTTGTTGTTAACATCACGCAGTTTTCAAGCGGCTTGATTACCGCCAGTGTTGTCAAAGATATCAGTTTGCAGGTGCACTAAATAGTTTTCGCGAAACTGGTTCCGTAGGTTTTGTTCTTGACTGACAAACGCATTATCCGTGTCAGCGGTAAAGCCGTTTAAGCGCTCTTTAATATCAGTATCATAATTGTCGTCATAAAAGGTGCGCAAAACGGGTGCTAAACGCATTGCTAGATACTGGTTAAAACTCTCGCGGTTAATTAGTGACTTTTGCAGAATCGAGCTAAGACTAAAGTCAGTGCGATCAGCAAAAGCACTACTGGGCTTAAAAAGCCCCTCCACAGTGCTGGCTGTAGGCTGCGCACAGGCAACCGCGACTATACCAAAAGTGAGCCCGATTGCGGTGAACATAAAGGGCCACTTGACTTTACGCGTAAACGTATGCATTCGCGTTCAAAATTTGGTATAATTCTAAAATTTAACCACTTTCTTTGACCGGGGATGCGGCTCGAAATTGAAAACGGCTTGGAATTTGTGTGTGATCCCTTTTTAAATGAGCGCGGCAAAATCCTTTTTTTACACGCCTTTACCGGTAATATCACCAATAAATTAAGCTTTCGCACCCACTTTAAAGATTACAGTTTTTACGGGATTAACTTTCCCGGACACGGTAACAGTGTCATTCACAACCAAAGTGAATTAGACTTTAACTTCTGGATTAAGTTAGTGCAACAGTTCTTTAATAAATACCAATTAAAGAACGTGGTTTTATTTGGTCACTCGATTGGTGGCGGACTAGCGATTGCCCTCACCCAAGTTTTAACTAAGGAACAAATTAAAGGCATTATCCTCGAAGCGCCCCTAAACCCGGGGATTCGCGCTACCCCACCAAGTATTATTAGTGCCTTGGTACCTGACACCAACGAAGACTTTGAAGCGGTACAAAGGGCATTGATCTACAACATTGAACAACGCTTTGGGGCGAACTTTAAGGACTTTTGTGCTAAACAAAAACAGAAGATGATCCAAAAGTATGCACCCTTAAAGGTAATGTTGCAACCCGAACAAGCCGAACAACGGCTCCAGTTAATTGATGCGGCTTTTAAACGGTTAAGTTACCCAACCCTGTGAATTCACGGGCAGGAAGATGGCATTGTACGTTACTTGCCCTCGAAGGCTTACCTGGAATCCTTGCACAATCCTTTAATTGAACTAGTGGGCTTATCCAACACGGCCCACACCACTTTCTTTGAGCAGCCCCAGCAGTTTCTCCAACTAGTGGAACAGTTTTTAAACAAGTTAAACAAATAAAAAAACTAACAACTTTAGTTGCTAGATCTCTTATTGTTGATTGATGTTTAAGCGTAGTTACTACTTAACCAAACGCTTGTACCATTCGACCACGTACGATTCGTTAATACCAGCAGGGAGTTCACTACGTTCTGGGAAGCGCACGTATGTCCCTTCAAAGGTCTTGTTGTTGGTTTCCACAAACGGGGAGACCACACCGCTTTCGCTCGCCGCCTTAACAATTGGAATCTTAATTGTTTTGGCCTTCAAGCGTACCTTATCCCCTGGATTGAGAATAATGGAAGGGGTGTCCACGGTACGATCGTTGAGTAATACGTGGCCGTGGTTAACTAATTGGCGCGCACTGCGACGGGTTGGAGCAAAACCCATGCGGTACACAATGTTGTCTAAACGTGACTCTAAAACACGGAACAGGTTAACCGCTAAGTTACCCCGTTGTTTTAATACCAGTCTAAACAAGCGTCTAAACTGTTTATCAGTAATCCCATACATGTATTGCATCCGTTGCTTTTCTTGCAACTGTTGTGCATAACCAGACATAGTGGAACTACGGAAACGGTTCCCGTGTTGTCCAGGAATCGTTTTCCGCTTCTTCCCTTTCGAAAACTCCTTGTTGTTTTCTAGTAACGAAAAACCTAAGCGGCGTGATCGTTTGAAAATACTACCAGTGTATTTCATGCGGTGACAATTTTAAATTATCTTAATAATCAAGATTAAAATCCCCACCGTTTTCCATTAGTTCATCATCCAAGATACTAGAGAGGTTAGAGGTTTTATCTGGAGTTAAAGTTTCAAAATCAAAGTCATCTAACTTCATTGGATCTAATGGCTCTAACCCGTGGATGTTCATGCTGCTTAACTGATGATCAAACCCATCTAAATCAAGGTTTAACCCACTGTCTAGGTTTTGGTAGACAAACTCATCCAATTGTTCATTGGACATCTTGTCAAAGCTACTGGAGAAGTCCAAGAGATCTTCAATAATGGCTTGCGGTTCAGTCTCAAAGCTAGACAATGTTTCCATGCTGTTTAACAATAAAGGATCCTTTTCCTTCTTGTTAAAGCGCAACACACTGTCTAGCTTCGCCATCCCGTTTAAAACAGTATCACTGCAGAGCTTATAAACGGCTTTAAGTTCTTTTAGCGTAATCTGGATGTTTTTAATCTTGCTTTCGTTTTCCAAGATCGCTTTTTGAATGTTGCGCACTAACTCACCGGACTTTTGCAATTGTTCGTAACTGTCACTTGGACGGTGAGCAATCGTAAAGTCGTTGGATAAAGAGCGTAGCAGTTCATCCTTTTGGTACACCGAAAGGTCTTTAGCGTTTAAAAACTCCGCTTTGAGCTGTTCGTTTTCTTCAATTAAGCGGGTCAATTCGGCTTGTAATTCCTTTTTGATTAACAAGAAGCGGTTGGTAATGTCGGTTTTGGAATTAGTAATAAAGTCACTAATTTGGACAAACGACTTTTTGACCACCATTTGGTAACTTGACATTTTAAAGATGTCATCGAAGTTAATGGCAGGGTTAGAACGACCTTGCTTTAACTCGGCAATAATGCTGTTAATGTCGCTGTTGGCAAACAGATTAATTTCAGTAGTTGGCTGTAACTCCGTTGTTCTGAGCTGTTCTACCATCACTGGCATGAGTGGTTTGACTGGTTGTTGTACCACATCAACATTTGATGAAGCCTCTTTGGTTTCCACTGGTGGAGTGTCGACAAAGATTTCGATCGCAGGTGCACTTTCGACAACTGGGGTAGTTGGTGTTGAAATTGTTGGCTCTACTACCCGGTTGACCGTTTGTATGTTAATGCTAGATACAGCAGGTGGAACAGGAACTGTTTTCGGTTCGCTTACTCGTCATACAACCTCACTGTTACTGTGACAGTGGGTCTTGCTTTCCATACCCACTTCTGGTGGGGTAAAATCATCAAAGCTGTGGGTACCGACAGCACCGTGTTGATTGTGCTTAAAGTCCACAACCTCAACTAATGGTAAGTCAGATGAAGTTGTATTATTGAGTTCAATTTCAAACAGTGGTGGGGCAATTGTTGGTTCAACTGGACTGGTTGTAACGGTAGGAGTTGATTCCGCCACTTCAATTTTAATTGGGGTAGTTGGTTGTACTGGTGCAGTCTGTGGTTGGTTTTTTACTGTATCGGTGAGATGTAAGACATAATCCATATCAGCAACGGGTGCTACTGCTGGTTGAGGTTCTGCGGTAGCGGTAGCTTGGCTTTCTACTACTGTTACTGGAATTACTTCTGGTTGTTCAACAGGAGCAGCTTCTGGAGTTAGATTCCATTCATAAACCGCTGGTTGAGGTGCAGCTTGTGGTTGTGGTTCAACTACTGGGGTTGGTTCAGCCACAGCTTCTTGAACAGTTTCAACAACTGGTTCTGGTTGAACTTCTACAGGGGTTTCAGGTTGTGGTTCAACTACTTGTTCCACTTCTACAACAGGATTTTCAGGTTGAACTTCTAAAACTGAAGCTACTTCCGGGGTTGTAATTACTTCTGGTTGGACTTCCACAGGTTGTTCTGGTTGTGGTTCAACAATCGGTTCTACAGCTACTTCTGGTTGAACCATTTCAGATTGAACTTCTGCAACTGGAGTCGATTCCACAACCACTTCTGGTTGAACTTGTTCCGTTGGAATTAATTCAGACACACCTGCCACAGCTGGTTCTACTGGAAGTGGCTCGGCAACAGCTTCCGGTTGATGTTCTACAACAGCTACTACAGGCTCTGTAACTACCTGAGGTGTAATCTCTGCAATAGTTTCAACAACTGGTGTTGGTTCAGTTTGTTGTGCTTCTGTAACTGGCTCTGCAGCTGCTTCTGGTTGAACCATTTCAGATTGAACTTCTGTAACTGGTTCTGGTTGAAGCTCTACTGCTGATTGTTCAGCTGGAATCTCTGGTTGGGTTTGTACAGCTTGTGGTTCAACTTGTTCCAATATTGGTGCAGTTTCTCCAATTGGATCTAGACTTACTTCTAATTGTGGCTCTACAGCTACTTCTGGTTGAACCATTTCAGCTTGAACTTCCACAGGTTGTTCTAGTTGTGATTCAACAATTGGTTCTACAACTACTTCTGGTTGAATTTCTACTGCTTGTGGTTCTACTGTTGCAGTAGTTTCTCCCACCGGTTCTACAGCTACTTCTGGTTGAACATCGTAAACAGGCTCAGCAGCTACCTCTGGTTGTACCTCATAGCTAGGTTCAGCAACTTCTGGTGCTGGTACATCTGCTTCCAAACCAAAGTCTTCTGAAGCTGTAGGTGTGGTTTCAATGTTATCCTCAAAGCCAAACTGTTTCTCTTCCGCTTCGGTTTGGGGTAAAGTCGAGATCCATTGATCACCCTCAAAGTAACCAGATCAAACCCACTCCCCAGCTTCATCATAGTAACCATAAGCCTCATTACCGACATACTGAAGGTAATCCGGTTCTGCTTCGGTTTGTTCCTGTTCCCCCGCTAAAACTTCCACTGTTGGTGCATCAGCAAACACGAACGGATTACCATTGGCGTCAAACAACACGCTAATGTCGTATTCCGTGTCACCATAAGGATCATAGTAAACTCCAGTTTCACTGTTAAAGGCAATGTAATAACCATTGCCATCACCGTCATAGGCAATCTTGACTTTGCCATCTTGAACAGTTAATTTACCATCGTACAAATTACCAAAGATCTGTTCGGGATTTTCCTCAGTGTAATCCTTGTCTTCAAAAACTGCTTCTTTGCTTTTCTTCATGAAAAAAGAGCTTACAAAAGTGTAAGCATGGTTAACTTTGATTATAAAACTTTCTTTAAGGCCTTATCTTTTCCATGAGAGGTTGTATACCCTTAAATAATTTCTTAAATTATTTTCAGCAATCCTAATTAAGGGCTTAAACAAGGCCAAGTACAAGGGCATTAACAACGCTTGTACCAAGACCCTTGGCATTAAAAAGAAATAGCTACCATACTTAACGAAGTTCTTACTTGGTTTACCACTTAAAAAGACGAGGTATTCAATCGAGGCTATAGTGCCAATTAACAATGATACCACTAAGGTCATTAGTAAGGCCATCATTAAGATGTAGAGGTTTAAGATCATCTCTTTCGGTTTTTTGACCTTAGTGTACTTTCAACCCAAAAAGATTTCTAACCCCACAAAAAAGCTAATAAAGGCTGCCATCGTAATGCCTTGTAGGATGTGGACATTGACATCAAAGCCCCGGCCAAAGCTCTGGTTACCAGCAGCTTGAAAATTGCCATCAGTCAGGTACATTAATAATAAGACTAGGGTCAAGACAAAGAAACCAATCAGAATGCTTTGTAAGATCCAAAAGTCAATTTTTCAGTTAGTACTACTTTGGCGTACCTGATAAACGCCCTTAACCAGCCCCGCTAAAAAGCAGAACATGGGTTTTTGAATCGCAAACAACCAAAACCAGGTATAGCCCCTAGTAAGCCAGTCCATCGTGTCGGCAATGGCGCCAAAGGTAAAGCCCCACACCGGTCCAAACATCCAACCAATTAAGGCAAAGGGAATTCTTGTGAAACTCACCTTGAGCACACTAGTGACATTAATGGAAAAAATATTAAAGATAAAGCTTAGTGCCATTAACACCGCCGCTCATACTAACAATTGTAAGTTCCGCGTTACCTTTAGGGGGTAATACGGAAACAAAAAGATAAAGAAGGCGTGGTACTTAGTTTTTAATAGTTTGAAGGTCTAGATTACCCAAAAACAGCTGGGGGTGTTAAATCATTGTCGTTGTTAGGATCACAATCCGTTTTAAATGGCTGTTGCATTTCCTCTTGCACGTAACGGTTCAACACTTCGTTTGTTTGATCCGAACTTGGTTTAGGACTCAAAACAAAGGCAATAATACTAATGAGGGCAAAGGGAATAATGCTCGCTAGGTAAAATATGGAAAAACCAACAAAGATAGTTTTAAAGGTCTCACTGTTAATTTTTAATTCATTAGACCTTAAGGCATTTAACAGAAAAGAGGGGTTTAAAATACCACCTGTAATAATGTTACCCCCAGGTAGGGCATTGAGTGCCACAAACAAGAAGATTGTCCAGAAGATCCACTTAATTCTGACCTTATACCGGTAGTTGTCATTGATCCTGGGAAAATCAGTTACCCCAAACTGGTGCTTGAGAAAGTGCATTGCCTTAAAGGTTTGACTTCTAGTAGCAAAACTGTAGGGCTTACCTTGAAAGGCTAAAAGGAAGGAAGTACTACCAAAACCCAAGAAGATCAAGGTAATTAAAGTAGAAGCACTAACAGGGAAAAAGAGGTTAATGCCACCGTTTAAATCAACTAACGCTGGCCTGTCGGTATTATTCTGACCAGTAACCGCTTTAGTTAGGTTACTGTCAGGGTTTACATACCTGTAAAACAAGAGGTTTACCACCACGAGGTAAGCAATGGCGGCCGCTCCAATTAACAACATGGAGATAAAGATTGCTAACTGGGCTCACTTAATTCACTGGCTTTTAAACTGGAATTCAAAGATTTTGCCTACCATCCGTTCCGAGGTGACCCGCATAAAGGCACGTTGGCGTGCACGTGCTTCGAGTGATTCGTTGACAAAGTTTTCCAACTCTGGGCTTACAGTACCAGTTGTATGTATCCGTGGTTGGTTTAAATCAATTGTAACCGTCGGTTTATTAGCTCCATTGGTTGTTTTTTCAACAGCTGTTGGTTGAACGCTACTTAACTTACTAAAGCTATTTAAATCACGGTTAAAGAGATTGCACACGGCAATTGGTAAATAGTTAGCCAAGTCCGTGGCATCAGTTAAATCAAGTTTGAGGTTAAGTTCGGTGTTCGCTAGTTTGGTTTGCCCTAAACTAACAAAGCGCGCTTCTAAGTTTTGAAAAACAGGTTGAAAGTCCTTTTTCTTGGTTTTAGCCTTAGCTTGCTTTTGCTCTAAAAAGTACAACAGCTTTAACTGTTCGCGAATTGTTTTAGCAAACGCCGGGTCGCTTTGGATCCGCTCTTTGAGGGCATTGGCATTAAAAACAATGTCTAAATTGTCTTGTAACTTGGTTAAAAGATCGCTAAAAGTCATCTTGGTATAGTTTACTTTTGCGCAAAGAATCGCAACACGAAGTGCTTTAGCAAATGGATTGCTAAGCCATCATTGCGCTTAATTTTAATATCCAGCTGCCAAATCGCATTAATTAAGGCCGTTAATTGGTTGGTGGATCAAAAGCGGTACTGGCTTTGAATCGCCTTTAAACGAAAGGGATTGACATTTCACTGTAGTTGGAAAACAGCTTGTGGTTTAACGCCTTTGACCATCAAGGCCAAAAGTAGTTCATTTGCTAAAAATTCTAGGAATTGCGGTGGTGTGAGCTGCTTTGGTAAAAACAAGCGTTCAATCAACTTTAAGGCTTTAGCTGTTTGCAAACGCACCATGGCGTCAGTTAATTGTAAGATTTGACTGGTTTGGTAATCACACAAAACGGCTTCTAACATTTTTTTGTCCTGTAATTCTGCCGGGCTGAGTAGAGTTAACTTACGCAACTCCTGTTCAATGACCCCTAAGTTAAAGGGTAGAATCGTGGCTAAGTGGTCAATTAAATCAGGGTTTAAGTTGAGTTTGAGTGTCTTAGCGGCCTTACTGATCACCTGGTGGATGGTTTTAGCTGTGACTGGTTTACAAGCATACTTGGTAATTGCCCGGTCATTAATGAGGGCATTAGCGGGACTGTCGCTGTAAATGGTGACCACCACATCAGTATCTTTAAGTGTTTTTAAGCAGTGTAATTCAGCCTTAGTTAAGTTGTTCTTTTCCAAAAAACTGCAGTTGTGAATGAGGATCGGTTTAGTGTCCTCAGCAAACAGATTCTGGTTTAACACCACATCATACAGCTTATTAACATCTTGAAACCAGACCTGTTTACAAGCGGCTGGGTTGAGCAGTTGGTTTAACTGCTGGTGAATTAACCCAATATCGGCGCCATAAACTACGGTCATAGTAAAAGACTGTACCCACAGTATACAACAAGCCCCACAGCCCATAATAAACCAGGTAAAACACCCAGTTAGGTTTGGGTAGATTTAAAAATACGGTCGTGTTTAAGGCTCACTGACTCAACCAGGGAAGTGGTCAGTAAAAACCGACCAAACTGTTGCCCATTCATGGAATTAGCGGTAACAACAGTCAGCTAGCACAGAAGTAAAACAAGGCTATGGGAGTAAACAAGAGGTTATGAAACACACTTAAAGCGTTTAATTTTCGATTTAAGTGCAAAGTAATCGGACTGACCACAATGAGGATTAAGGTGGAAGTTAACACCGCTTGCAGTGGCTTTCACAGCTTCACCTTATTAATGGCAAACAACACAAAGCACGCTAAAAAGGAAAAGTTAAAGCCAAAGTTGTGTAACGCTCCCGGACTCATTAAGATGATTAACAGCGCCGTTAAGCCCAAATTATTTTCGGGTAGTTGCTTGGGAAACATTTGCCGTAACAGCTTTGAGACAAAAACCCGTAAAGCTGAAAAGGCAAATCCGACCAAAAAGAGGTACAGCAACAGCATTACAAAACCCGTTATTTTATTGATGTAAAAGCGCTTGTACAACCAGCGCTCCATGACACCAAACAAAAAACTAAGGTGAAAACCACTAATCACAAACAGGTGGACAATATTGAGCTGAATGGCACTCTGATACAGTGTGTTTTTTGTTGTTTCGTTAATTAAAAACAGCTTTAAGTACTGATTAAAAACACCACTAGTGTGTTGATCTAGTCAACTAATAAAAGGGGTGCGAAGTCAGTTCGCTTTCATTAGTTTGGCTAGTTGAAACTCTGGCATTAACTGACAGTATGCTAGTAAAGCGGCACTACAACTGATTAACAGTAAGCCAAACTTCCAGTGTACCGTTAAACTAATTAGGTTAACTATCACCAAAAAACTCGCTAAAATTACCCACTGATTTTGTTCGATAAAGAAGTAAGCAGGGGTAAAGGTGAGTAACGTAAAAAAATAAAACAGCTTTCACTTCATGCTGTTTTATTAATTGGTTAATTAGTTTAGAAATGCCAATTAAAACTCTTTACTCCAGCTGGAACGGAGCGGAAAGCGTTGAGACAAACCACCACGTAACGGTGAGCGGTAACGCGGGGTGAAACTGGTGTAATTGCTCCCTAAATCAAAGCGGTGCGTGTTGAAAAAGGAACTGCGGTTACGCCTTAAGCTCGACAATTCCAATGGTCTTCTAAGTGGGTAAAAGCTACTTGGACTGCGGTAAGCGCCAAAGCTGTTAAGGCTGTCGAAGTTCAAACTCGCTGGGTTGCGGTAGTTTACAGCCATCGGTGTAACACCAGTAAACCGACTGGCAATTGGTGTTGACTCTACTGTTGGCCGTGTAAAACGTGCTCAACTGTTGCGGATTGCTGGAAGGTTAAACAGCGGTTCGTTGTACTTGGTAGCCACATATTCACTCGGTGGAGCTAACTGGTAGCGCTCGTGCGGTAAAGGCGTACGGGTAGAAGCATCGTGGAGGTGACCCAAACTCGCTAGCTTTTCCAACAATAAGGAACGGTTGGTGGTGTGAATGGTAATTGCACCACTTTCATTATTAACACAAGTTGACTGACACGGTTGTGGTTCCACTACCGATGCAGGGGATGGTTTTCATTCCGGTTCGGGTTGTGTTGGACGGATAATTGGTTGGACTACCACTTCTGGTTGAACTACAACTGGTGGAACCGCAGGAGTTGTTTGGACTACCTTAGGTGTCACTTTAGGTGTAGCTACTTGAGGAGTAACTACTACCTGTGGTGCTGGCGTTGGTGTTACTACCTTCGGTTCTGCCTTAACAACTGGTTGAACTACAGCAGTTGGTTGGACAACTACTGGAGCAGGGGTTGGTTGTGGTGCTACTTTAGGTACAGCTGGAACAGGGGTTGGTTGGGCCACTTGGAGTGCAACTTTTGCTGTTACTACCTTCGGTATTTCAACTTTAGGTGAGTCAACCTCAACAGTTACAGGTGTTGGTTGTGGTTGTTCCACTACCTTAGGCTCTACTTTAGGGGCAGGAGTAGCTTCTACCACTGGTGTTGGGGTAGGTTCCACTACCTTAGGAGCTTCCACTACTGGTGCGGTCTCTACCACTGGTGTTGGGGTAGGTTCTACTGTTGGAGTAACCTCTACTATTGGAGCAGATTCAACAATAGGAACAGCAGTTGGCTCCACTACTGGTGCAACTGGTTCTGGTTGGAGTGGTGCTACCTCTATTGGTGCTACAGCACTAGCTTGATCAGGATCGCTGTAAGCTTGGGAATCATAGTAAGCGTTCGGATCGTGAGCTTGATCATAGTTCTGTGTATTGTAAGCATTAGCATCATAGGCTTGATCATACGCTTGTTGATCAAAGGACTGATCGTAAGCTTGCTGGTCATAGTAAGCGTTCGGATCATAGTTTTGGTCATACGCTTGCTGGTCGTATGTTGCTTGATCAAAGGTCTGATCATAAACTGGTGGTGCTGTAGTATCCTGATATTCGTCTGGTGCTTGAGGTAGTGGATCGAGTCCGAACAGTTGATCAGCTTGCTGTTCGGGTGTAGCATCGGGATCCACAACTGGTAAACCAGCATCAATGTCCGGATAGCCCTCACCACTCAATGGTTCGCTTGTAGTGGTATCTGCACTTGCGGTACTACCACCATAGTTTAAATAGTCCACTAGGGGAATGTTTTCCCCATCACGGTAACCACTCACATCCAAAACATTGCCATCTTGGTCGTAAAAGGTATTGTTGACCTGGTCGTATTGGACTATTACCTCATTGTTGTTGCCATCAAAGGCAGTGAGTGGAATAATCTTGGGGGCAAAGCTAATTGGTTCATCACTGTCCAATTCGAACAGAGGATAATCCGCTGGGAGCTGTTTTCTTTTTTCCAAAGAAAAGATGTTCCCGGTTTCAGGATCCTGGTACAGGGCGTGAAGCTTATTTTTAGCATCACGACCATAAACCACATTAATGTCGGGATAGCTCTTTTGGATCTTTTGTGCTAGCTTACCATACGCTTTCGCTAGCTTTGCTCTTTCTTTATCTGTCATCTTGAAGCACTAATTAGCGTTTTGGTGGAAAACCGGGTTGGGGTGGCATGCCAGGACGTGGTGGTTGCATTCCGGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGGTCTTGGAGCCATACCGGGGTGTGGCGGCATACCTGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGGTCTTGGAGCCATACCGGGGTGTGGCGGCATACCTGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGTCTTGGAGCCATACCGGGGTGTGGCGGCATACCTGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGTACGGGGACCGAAGTTAATTTGGACTTGGGGTTGAGGTGGTACTGGTACTGGTTGTGGTGCTGGTTCAACTTCCGCTTCAGCATGGGCTTCAGCAGCTGCTTGTTGTTCTAACGCTTGTTGCTCTTCTTGTTGGGCAGAAATGCGTTGTAGCTGTTCCGCTAACTGTTCTTGGCGTTCCTTTTCCTCCAATAAGCGTTTTTCCTTGCGCTTCACAATTGGCAGTCCTATAGCAAGGCCCAAGATGATGGCTAGGGCACTAAAACCAAAGCAACCAGCAACTGTAGGAATAAACCATGCTTGCTCAGTAATCGGCTTGCCCTGAATTTGTACTGTATCCTCTTCTGCGTGTAAAACAACGTTAAGGGGACTCAATTCACTCTTAACTTCTGTCAGTTCGGTATTGTTGTGCTGTACCAAAATTAAGGTTGCAAGCACTATTAAAGCGCTGAACAGCACTAGCATTCAGGCTAATAAAAACAGTTTAAGCTTTCTTCGAGGTGGTAACTTCATGCACTAATTAAAGGGAGACACTAACGACGGCGGCGACGGGGTGCTTCATCATAGTAGTAATCATCATCACGACTTTGTTGTGCCCGTTTTGCTTTACGGCGCTTTTTGATGATGGCACGGGTAATCTTGGACAGGATAAAGTTAATAATTAAGGCACCTATAAGCCCTGAAACTAAGGCCATGCCCAACGGAATAATGTACTTAGTAAGGTAGGGACCAACCTTTTCTAAAAAGGCGGGGATATTGGGTTTCTTTAAAAAGTCGACATACCCTGGGATTTGGCTAAACAAACTACCATCAATCGGAAAGCGGGGATCTAGCTTGTTAGCATTATTAGCTACTTGTTCTGCTGCTAGTATCAAGGCGTGGGACTGTGATGCTGCAGCTGCTTTAGGTGCAGTTACCGTAGCGCCTACACCACTAGTAGTAACCGTGTCAAGCGCTTCCTTAGAGAAAAAACGGTTAAACACATCCATTGGTTGTGCTAGTTTAAAGGATGGGCTAACAGTACCAAAGTAAAAGATTGCTAAAAAGAGGTAAGCAATTGCTACCGTCAGTACCGCTAAGAAGGCTAGCTTAAAGAGCAATTTGAAGAAACCCATTGCTAGAGAAGAGTTAAATATTGGGAAAATTTTAACTTTTCAAAGCTTTAATTCCCCACTTACAGCTTAACTTCCTTAATTAAGAGCTTTAAAAAGCTCTGCTTAAATTACGCGTTAACAAAATAATGCGCAGGATAAAGTAGACCAAGTTCATGAAGCTACTTACCAACCAAGCGCCAAAAAAGAGCGCACTACGCCACATTTGTGCTTTGGCTAAAGCTGGGTCTTGACTTTCATACAGTTTCACCAAGTCCTGTTCGTTGCGAATTTGTAGACAAACAAAGTAAACCGATAACAAGGAAAAGATCCCACCAACTATTAACGAAAGTCCGGGGAACAGTAAATCCAAGATATTAGTGCGACCACCGCGGAACACCGTAAAGTTCAACACAAAAGTCAATAAGCTCGCAATTATGCTAATAAAGAAGGCTCAAATAAAGATACGAAACAGCGTTTGGGTTTGTTGGTAACGGCGTTGCTGGCTCGGTAATGCACTCAAGCCCCATACCGTCCCATACACTAATGCTGCCCCTAAAAAGGCAAAGACAATGTCACTAAACTTAAAGTCAATCAATTCACGTAATTGTGTGGTGGCTAATGTTAACAATACTCCTAATGTAATCCCATAACTAATCACATCGGCAATAATTAAGCCCCAGAACCACGCTAAGGTCGTGTTACGGTTCCTTAGTTTAAAAGCCGTGACAAAGTAAAGGATTAAGGAAACCAAACTAGCAACAAAAGCAATCGTACCCAACGTTCTTATATCACTAGCCAGTGTGCCAAGGCTAGTAGTGTTGGTTAACAGCTGTTGTACCCCAAAACCAATTAAGAAGATAAACAGAATGCCAATCGCGGCAACAGAAAAGGTCAAGCGAATGACCGAGAACCGCTTGTGATGGACAAACTGTTGCTCGGAAAAATTGACCTGATTATTAGCTTGATTGATCATGATAAGTCAACTGGGTCAATTTTAATTTGTAAGCGCTTAATTGCTAATAAATGCAAAATTATAGAAAGGGGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAACACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCACGGAGACTTACCCAAGTGGTTTAAGGGGGCGCTCTCGAAAAGCGTTAGGTGTTTTTGCATGCGTGGGTTCGAATCCCACAGTCTCCGCCAAAATATTAACTGTATCAATTAAAAAAGCACACCTTTTGGGGATGTGCTTTTTTTAGTGTTTCCTAATACTGACAACTATTTATTTTCGGGAATAAAACCATAGTCACGGATGGTACCGTTACCCGATGGCGCACTGCGGAATTGGAACCATGGTTTGAGCAGCGTTGGTGATGTTTCGTTCAACCCAGCTGCTGGTACGGGTAAAACCGGGAAGTAAGAGTCGACTACGGTAATTAAGAACTTAGCGTTATCGGTGTCTTTTTCCGCTCAAATACTGTTAAAGCGGTTGAGCTGGGCGGCGTTCTTATAGATATCTTTCAACTCGTAACCAATGAAATCTTCGTTTTTACTTGGTTTATTTGTACCGTTCTCTATCTTGTCACGCTTTCCAAGGTACTTATTTGGATCTTTGATAGCTTCCTTTACTTTTTCTTTGTCTAGAATGCCATTATCCACTAAGAATTTGAGCAAGCTTAAAGCCTTTTTGGTGAACTCTAAAGATGCTTGAGTGCCGTTACCACTAGTTGTAGAACCGGTGCTTCTTTGTGCAGCTTGTGCTTTAGCATCACCAAAGTTCTTTAGTACGTAAGGGTCATCTTTGAAGAGGTCAGCGGAAGTTACGTCATCCCACTTTTCTTCTTCAGTATTACTCTTGCTGCTATCTTGAGATGAATCTGCTTTCTTAACAGTCACATCAGATGTGGTTGAAGCTGTATTTTGTTGATCTATTTTTTGTGTGAATTTACTATGTACATCTTTTTGACTCGCAGCTAACAACAACGTACGTACAATGTGAGCGCCCGAGGAGTAGGTAATAACATCACCGCCACCTTGATCGTTCAATTGGGTGGCTGCGTCCAATCAGGTACCCACACCATCATAGTCAGGGCTTCAGGAACTAAAACCTAACACCGAGGACAACCCAATGTACCACTCATTTCGAGTTGGTCCTTGGGCATCACCTAAGATAATTTCTGGTACTATTAGTTTTTCTCCGTTGTCAGCAGTACCCAATTCAGTGAGGGCTTGCTTCACGGCGTTGTAGTAATCCACCCCGTTAGCGGACAGCGAAGAGTTCCCTAACTTCATGTTGAAGTGGAGGGGATTGCTACCAGAGAAACCAACCTTCTTTAAAACTTGAACAAAGCTGTGCTTTGTTACGTTTACACTCTTAGCTTCTACTGAAGAGGTTTGGTTTTGTTGTGTGTTGTCTGATGTGTTGTTGTTCTTAGCAAACGCTGCAGAGGCAGCTGCTCCAGCCTGACCACTTGTTGCTTTACTGCTATAATAATTAATCTTAACAGTCGCACTCTCCAAAACGTTTTTATTCTTTTCGTAGTTACCTTCACGCTGTGGAATCAAGCCCAGGCGCTGTTCCTTCTCGATCTTATAGTAGTAATCGTTCATCAGATCTGAACCTTCGGTAGTTGAGACAGCTTTTTGTTGAACACTTGCACCATTTTTACCCTTTTCCTTAAAGATGCCAAAAGGTACGATCGAATAGTTCAGGTCACCGGAGTTTGGCAACAGGATAATCGCTAGGTTTTGTCAGTTAATTAAACCGTTAATGCCAGCGCGAATCGTTAACGCTTCTTCTGACTCACTGTTGTACAAGAATTTAGCCTCGGTTTCGGTAATGTTGGAGTTAGTTTTAACGGTTTCACCTTCCACTACGTAAGGTCGTGATGAATAGGTAATCTGGGCACTTTGCGCGGTCTTTTGCACTTTAACCCAGCGTAAGTCCCCCGTACCCGCAAATCGTGACCTAGCATCCTCTTGTTGGGAGTATGGCACCTCCGCAGAAGATAATCCTCCAGCGATATAGTTGGAGTAAAACACTTCAGGGGTAGCGCCTGGTTGGAAGTAGTTAATCATCGTTGGGATGGGTTTTTCGTTTTCTTCCCGCGTTTTTGGTAAGTTGGGGGTTATATGAGTATCATACTCACTGTTACGCTTAAAGACAATCTGGCTCTGGTTAAAACTCTCGACATAGTAAGGCCCCACTGATCAAGTATCCCGTCATGCGTTAACCCCACCACCATAGATACCATCAAAGTTAGTTTTGGTTTGGTCTAAGATTTTCGACCCATTCTTTTCGTTGTAGACCAATGGTGAGTCCTTACCTACCTTAATTGCTTTTACCTTCGGGTGGGTATGAGGAATAGGAAAGAAGAATTCCTTAGACATCATACTCAACAAGAAGGGGAAGGGCGAGGTGAGGTAGACGTTGAATTTGCTGTCATCAGTGCTTTGATAATCCTCAGGATTGTAAGCATCATCCGTTATCTTAACAGTTTTTTCTTCACCATCTTTTTCATCAGATGAAGCAACCTTCATTTGAACTTGGGTACCATTCTTCTTGGTCATCCCCACGGTCTTTTTCACATCCAAACCCATTAAGTCAATGAAGTAACCGTTGCGGTTAAACCTGAGTTCCGATGATAAGATGTAAGCCTCAAAACCGCGTTCAAAGTCTTTGGAAGAGAGTTTGACATCATTGCCACTAGCGTCCTTTACCGGTTTACCGTTATTGTCGACCCATTTGATACCAGTATCAATGGCGAATTCGTAACGGGTGGCCTTTTGCGAAATTTCTTGGAATTTTTCTTCCTCAGTAATCGACTTCGATTGTTGATTATTTTGACCGTTACTTGAACTTTGTCTTTTAATTTCACCAATGCATTCTCAATCATCTTCACCACTAGTGCTAGTACCACTAGAAGTGGTAGTGCAAGAAGAATTAGTTGAAGTTTTTTGACTTGAAGTAGCTTTATCCTTCTTCTTATACCCCTTCACTGAGGTAGCCAATTCCAACACCAATTTCTCTTGGGTTTGAGTGGTTACATTGACACCATCATCTTTGTTGGAACCAGTGGCAATGTAAGTGGTTAGGCCTGCATACTTGCTGCCAAAAAAGGAAGAGGTCGGGGACACGTTGAACATACTCATCGGTACCGAATTGTTCTTTAACAAGTCGGATGCGGATGAGGTGACATACACATCGCGGGCTTGGGCAGCGCAGGCACTGAGTGCTACTGCTGAACTTACCCCTAAGGTTGATAACAAACCCCAGTATTTTCGCTGAAATTTCATGGATCGTTCATAAATGTTGTTGATGATGTTTATTGCTATATGAAAAACATGGCATGAAAGCCATGTTGAACCATGAGCAACATACTCTTAGCTGTGAGCTACTAAAAGTGATTTTATTCTAATTTGGGAGCTTGCTAATCTGCGAAATTAAAGCTCAAGTTTCAAAAATTGCTGTTGCAATAAATTAATTCTTAATTTAAAGGCTTCAGCAAACGCTAAAACTTGGTGCACTCGAAGGGACTTGAACCCCCACTCCGCAATGAAACTAGATCCTAAGTCTAGCGCGTCTACCATTCCGCCACGAGTGCATATTGGTGCATCTTGAGGGGCTCGAACCCCCGACCCAATGATTAAGAGTCATTTGCTCTACCAACTGAGCTAAAGATGCATTAAGGGAAGCTGGTGCCGACTATAGGATTCGAACCTACGACCTACTGATTACAAGTCAATTGCTCTACCAACTGAGCTAAGTCGGCCTGGTGGATTGTGAGGGGATCGAACCCCCGACCCTATGCTTGTAAGGCATATGCTCTCCCAGCTGAGCTAACAATCCAGGCTTTTGGTGCCGAATACAGGAATTGAACCTGTAGCCTATGCATTACGAGTGCATCGCTCTACCCTTGAGCTAATTCGGCTTGGTGACGCGTACGGGATTCAAACCCGTGAATGCACGCGTGAAAGGCGTGTGTGTTAAGTCTCTTCACCAACGCGCCAAAATTCTTGGCGGCCACAACAGGGATCGAACCTGTGACCAACCGGTTAACAGCCGGTTGCTCTACCGCTGAGCTATGTGGCCAATAATGCAAGTACAATTCTAGAATTTCTAGCCCACTTTAACATCTTCCCGCGGATAAGAAAAGCTGTTTCCTTGAGGGTGTTTTTTAACAAAAGTGAGTACCGAACTGCTCACCCCGACCTGCCCCATAATCATTAGTAAACCTAACGTTAAGTAGTTAAACAGGTTCCGTGTTGGCTCTAGTGCCATGATCTTCGTGGCCCCACTAGACAGTCCGACAGTTCCAAAAGCAGAAGTGGTTTCAAACAGGCTGTCGACAAAGCTAAGCGGTTGTTGCATACTTAACGGCAACAGAATGGCAATAAGAACCACGGACACTAAGCCAAAGACGGCTACCAAGAAGGCATTAATCACCGTTTGCCCGTCAATACTACGCTTAAAGACCTTGACCTCTTTTTGACCGCGGAACTTCGCTTTTAACGTCAAAAAGATGACCGCTAAAGTAGTAGTGCGAATCCCTCCGGCGGTAGAAGACGGACTGCCCCCGATAAACATCGCAATCACCATAATCCCCTTAATGATGTCAGACTCAGCACTTCAGGGAAACACACTAAAGCCAGCGGACCGGGACGAAATCACCCCAAACCACAGTTGCATCACCCGACTAGCCGCTGGTATGGGACCAAAGATTAAGCTTTGGAGTTCTTGATTGGATTTATTTTTCAGTCCCTCCACTAAGTAGGCCTTCTCGACATTGAGATGGGCAATCGTATTGGTCAAACTAGTTATAACAATAAACTCCACCATTAACAGCATTAAAAAACACCAAGCCGTGACAATCAGGTTAGTGAGTACCGCTAACTTCGTAAACAAACTAAACTGGTGTTTGTAATACTTACCATACAATCGCCGTTTCTTAATCGCTTCATAAGCATCAAATAACACCGGATAACCAATGCCACCAAAGATAATTTGGCTAATCGCTAACCACTGGATCACGATGCCCCAGTTAGTGCGATACGGGGCAAACGAATTAGCGCCCAACAGGTCAATGCCAGCATTATTAACCGCTGACAAGGAGTGGAAAAAACCATACTGAAAGGCTAAGTTAAGGTTATGAACTACGGGTAATCTTTTGGTGGAATCGACTATGAGGGCCTTCAATTGGTTGGACACCTTACCACTACTTTGAAAAACACTAGCAGACTCAAACGCGGGGATAAAATAAAACAAAATGGTGTACAGAAAGCCATACAGTAACTCCACCATAAAGAGGAATAACACCGACACCACAATCATTTCACTAGTGGTCCCTAACTTGGAACCACCCCGTTCTGATTGCAACATTAACTTCTCATAAAAGCTGTACTTGCCCTTCTTGTGTAACTTAAACAGTCTTCAAACAAGGAAAGCAATCACCACAAAACCAATGCCCCCTAACTGCAGTAAAATGGCCAACACCGTTTGCCCAAACACACTGTAGGTTTCACTCACTACCGTAGTAGAGAGCCCCGTATCACTAAAGGCACTGACCGCTAAAAACAAAGCATCCAAAAAGCCATAGGAAGCCGTTTGTTTACTGATGGTATGACCCTGTAAATCCACCTCATAGCTAATAACCTTTTGGTAGTCGGTTTTGAGCGCAAACGGGAGGAAAAGCAGTACAGCCCCCAACAGGATGCAATAGATGTAAAAGTGGAAAATGCGCTGGGAAATACTGTCACCCCACAGGATTAGTTTTAACCAAGCGAGTAGTTTAGCTTTCTGTTTATGAGCAGCTGTCATGTAAATGACAATATAAATATAATTTTACCAGCATATGAAACGTGCCGATTTTTGCATTATTGGCCTTGGTAGGTTTGGGATGCAAGTAGCACATTCTTTAAAAGAGAACAACTTTACCCTGGTTTTGATCGATAATGATCAACACAAAACCAATACTGCTGCCCAGGAATTTGACCATGTAGTGTGTTGTGATGGGAGTAACTTAACGGCGTTAGCTGAGCTCCAACTAGAGGAGTTTTCGGCAGTCATAGTTGGGGTAACTAACATAGAAGCGAGCATTATGATTTGTGCTAACCTTCGGGAATTGGGTCAAAAAAACATTATTGCCCGTGCTAAAAACGAAGTGCATAACCGGGTGTTACGTACTATGGGTATTAGGGAAGCTTTGATCCCGGAAAAGATTGTCGGCAAGAACCTTGTCATTCGTTTAATTCACGGCATGGAAACCGAAATTATTAACCTTGGCAATGACATCCTCTTTGTTCGCGCTCCGGTGAACAACAAGAAGCTCTTTAATCGTACGTTGGGGGAAATTAACATCCGTGAACACACGAATGCTAACCTCATCTCAATTATGCGGAATGGCAAAGATGTCGTCTTTCCTTTGGGTCCGAATACCCAGATTCAACCAGGGGATGTAATTACAGCCGTGTGTCAATTAAGTGGCGTGAACCAGTATTTGCGCTACATTAATCCTAACGACAAGAATAAGTACAAAGCGAGTGAATAATTAGATCACCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAACAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGAACGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAATCGTACCGCCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGGTTGTGGGACTGAAAGGCGCGCGATGTCCTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCAGAGTGGTTTGACCAGGGCCAAGCGGTCGCGGATAATACCCAAACCGGGTTTGATACAGATGACACCGATAATAAAAAGCAGGGCTTTCGCAAACAAGGCGAACAATCCCCTGCCCCCATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAATTAATAAATAGGTCTATGGATTAATTACCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGTCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAACTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGTCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCAGAAGTTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACGAAGGAGGGGAAGGAGAAAAACGAATCGGAGTGGAAGTCTTCCGGGGCGAGTGAGGCAAAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCTGTTACAGACAATCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCACAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGACTTCTACAAATGAAGATTTGTCGAATGTAATAACACAGCTTTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATAGTTGTGATGGGCTCAGACCGGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCCCACACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGGGCAGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGTGCTCGCCTGGTCGACCACGGCCGGATTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGATTGAACGGACCGATTAATGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCCAAAATTGCTTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACATCCGATGATAATTCAAACACCAAAGTCAAGTGAACCAACACCGCCTCGCACTACCTCCCCGTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGGAGAAGAGCGGAGCAGCGGAATGGGGTGATAACAATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCCGTTAGTTTGATGTAGAGGCAAAACTATGAATTCACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGCCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAGTAGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTTTAATACCTTCTAACTATTAATAGGAACTTTTCTTATTAATAAGAACTTTTTTGAAAAAAGTTTTGAAGTTGTTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAGCACAAAGACTCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCAGTGCTGCAAGTTTTAGTGACTTTTCAAACCTTGCAAAGAAAGGAGGTATTACTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATACATCATTAATCCAGAAAACAACAGTGTGGAATTCTCTGGCAAATTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAAACTCAAACAACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAGAGAACTTCAAGTAGAGCAAAAATTTCAGGGTGAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCGCTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAGTTTTTGGGATAATAACAAAAGTGCCAGCAGTTAATCTTAAAGATTAATTGCTGGTATTTTCATTCATTTGAAATGAACAAAAAAGCCATCCTATCAAAGGTGAAAGATGGACGGCTAATGAATTGAAATCAATTACTTAATTAAATATTCCAAGTAAATAAAGAGATTAATATTTGATGAGTAGCCTTCTGTATTATTAGCAAAGGTACTTAAAAATTTGTTCACACTTTTTTGACTAACATCATCTTTGCCTTTTAACGAAAATTTAAGTGAAGCGCTAAACTCAATTCACTCTAAATTTCTTGTAGTTTCTTTCCCACCAATAGTTGTAGAGTTGTCTTCTTCAGTTTTAAAGTTTGCAATTGTAAATGATTTTTTGGCATCAGGTTTGATTTTAAGGAAAAGATCAAAATAATGCTTATCACCTTTCTTAGCAGTGAAACCACCTGAAGAATAATCTCCAAATCAATTAAAGGTATAGCTTAACTTATCCTCAACTGACAAATCCTTAAAACTTTGGTATGTCATCTGGAAATGTTCAATCTTTCGCTCCATCGTAAAAGGATCCTTGCTTGCGGGATAAATTACATCGTCAAAATCAAAATCTGGAATACCTAATTTACCCTCTTCAAAAATCTGTTTTCAAGCATCTAACAACAATTTTGTTGTATCTCTCGGATTAACTTCGTAAGACTTTTTTAAAGAAGCTATTATGTTCTTTTTGCCTTCTTTAGTTTTAATTGCTTTAGACAAATCAATGTCTTTACCAGTGGTTTCTTTAATAAGATTTCTAAGATCTGCTTGGATACCAGTGCAAGCACTTAACAATGTTCCACATAATAAAAATAGAGAACTAAAGGAAATCCTTGACAACAATCTCTTTTTGCGCATAAACAGTAAAAATAACTCTTTGATTTTGCTTTTTTTTGGAAAATCCTTTTGAATAAGAGGAAACTAATTGAAATTTCGGGTTTAACATTAGTTAAAAAAATGAAATTTCTAATTATTTATATAACAACTATCTACTGATAAACGTCTCGATCTGAGTAGTTCTAATAATTACCAATTGTTGGTATTTTTATTCATTTAATGCTTTCTAAAAACTTTTTTTTGCAATTTTGTTTGCACTTCTCAATAAGCCGGTCAATTTATTTAGTGGAGGAACAAAATGCAAATCTTTTATTTTTTGAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAAAAGAAAGGAACATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGATACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGTTGACACTGAGACGACAAATAAAGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTGATTTTTTGGTCTAAAAAGACATAAATTTACCAACGGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTTGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGTACAGGTATGGGAATGGATATAAGGGGGTCGCTGGAGCGGGTCACAGCGCCACCAACGGCTCGCCGGCGGGCAACACAAGTTCCACCACGCAGTCCAATGATGTTGCACCCACTTTTAGCAATGTCGGCGTTGGTCTCAAAGCGAATGTCCAGGGCACGCTTGGTGACAGTCAGACGACGACTGTGGGTGGCCATCAATGACCCACCCTCGACCCAGCCAACCTCCAGCTCTGAACCGGCGCGGGGTGAAGGAATGATTCAAAGACGCAAAATAATACTACGACCAACGAAAACCACACCAAGTTCGCGAGTGCTACGGGCAGTGGTCAGCAGCAGGGTTCCACAACAACTACCTCCGCCGGCAACCCCGACTCGTTAAAGCAGGATAAGGCTGATAAAAGTGGTAATTCGATCAGTGTTCAAGAAGCCACATCGGGCGACAACTTGACCAACTACACCAACCTCCCCCCCCAACCTCACCCCCACATCCGACTGACCGAACGCTTTGAGCTTCACCAACAAGAACAACGCCCAGCGCGCCCAGCTGTTACTCCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTGAATAATTAATTTAAGGTCAAATAAAGCAATAAAAAAAGGATCCAAACAGATCCTTTTTCAAATAGAACGCTGCCAATAAGCCATGTTCTGTTTTAAAGTCAGTTATTTATCTACAGTAATCCTGTCATTACTTTAATTATTTTTCTTAAAGTAACATTCCCTTACCAAAATTTAGGTTTCTTGCTTGTGGAGTTTACCGCGTTTCATACTTAGTTTTCACTAAGCTCGTCTCTGTGGTACTTTACGGACATCATCATAGTCAAAGACCTTAGACTAGTCCAGTCGTGACGTCTATTAGCGTCCTAAATCTTATTTGTTCAATTTACACAAACACTACTTGCGTTCCAGCAAGTGCAAGCATGGACTTTCCTCTACTCATTGAGCAGCAACTGACCGACAGCGGCTAAAATTATAATTTGAGCGTTGGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTGGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCATTAACAACGCAAGTTGACCAAACCTTCCTTCGGGAAGGTTTTTTATTGCTTACGTTTGCGACAAAAAATGGCCTTAGCGCGTTGCTTGTTTCAGTTAAAGGCAAACAGTCCTAAAAAAGGCAATTCATACACCACAAGCGTAATACATAAAGCGGTGGCAAACGCTCCTAGTTGGTTTAAGCGCGAACTAAAAACGTTGTCTTTGTGAGAAATCCGAAAGAACAGTGACAGGTTAGGATTACTTTTTAGTTGCGTTGTCGTACGCGGGAAAAAGTGTTGGTCCGATAACACCAAAAAGAGCAACATCAACGCAATTCAAAAGCAATAAGCGACCACACTAAAGTAAAAACCAGTACTAAAAATGACCCGTTCTCACTTAGTGATTTGACTCTTAATAAACAACGAAATTGCTCAGCGGTAACCCAAAAAGATCATTAAAAACAAGCTAAAGAGGCCCAACGCTAGAATGGCAATACCAAAGCTGCTAATCGAATACACAAAGTTAGCGTTAAAGATGTCAGCTGGCTTTTTGACATTTCAAAACAAGTAAAAACCACTAATGCCCTGGTTTTGATCGTTTAAGGTAAACTGCTCGGTGTGAAAGATACCCACCAAGAACATCACTGCTGTCACAAAAAAAGACAGCAAGAACAAAAAGTAAGCAGCGGTATCTAACAGACCAAAACGGCTGTAGAAGAGGTACTGACTGTGTTTGGAAGCAACTTCGTATTCAACGGTATTAATTTCGGTGAGTTTATCGTTGATCTTACTCATCCAACCACCGCCGCTAGCAACGTTCTTTTTCTTGTTAGTCTTAGAAGGCACGCGGAATCTTGGCTGATAACCACACACTCTTGTGGTCTTTAACTAACACCATGTCCTCAATGCGGATGCCACCAACACCAGGGATATAAATACCCGGTTCAATCGTAATGACAGCATTTTCACACAACAACTTGTTATAGGACGTTGATACGTTTGGCATTTCGTGAATGTCAAGTCCAACACCATGACCAGTGCTGTGAACAAAGTAATCTTTGAACTCCGATGCTTCAATAATGTCACGGCACACCTTGTCAACTTCCGCACCGGTGAGTTGGGTATTAGCAGCGTTAATGCCGGCCATATTGGCTTCATCAACCTTTTTATAGGCCTTGAGCAACACTTCATTATTAGGTTTTTTACCAACTAAGAAGGTGCGGGTAATATCGGAACAGTAACCGTTGTAAATGGTGCCAAAGTCACAGGTTACAAAATCACCTGATTTTACCTTTAACTTGGAAGGCTTGTGGTGTGGATTAGCACCGTTCTTACCAGTAGCTACAATAGGGTCAAACGAGTTTTTTGCCCCACCAGCTTTGACCAATTGGTCAGTAATCCACTGCGCAATGAACAACTCCGTCATGCCTGGTTGGATAAAGCGCTTGAGTTTTACCGCTACTTTGCGGGTAATTTCCACTACCTTTTCAATCGCTAAGATTTCACTCGGGAGCTTTTGTCGGCGAATTTCCTGGGCGTTAATGACCGTGTACTGCGCACACAACTTCTTAATGAAATTTTGGTAGTTAAAAGTGAGGTAATCCCCTTCAATCAAGAGGTGTTTCACACCCACTTGTTCACATAATGCCTTTACTTGTTTATAAGTGGTAAATAATTCCACTTTGACTAGGGGATCAATTGCCGTTTTAGCGGCTTCAAAGTAACGCCCGTCAATAAAGAGGTTAACCCTTTGCTTGTGAACCACTAACCAACCAGCACTGGAGGGAAAACCAGTAAGCCAAAAGCGGTTTTGGTCTGAGCCAATTAAGATGGCATCAGCTTTATTGTCTTGGAGCAGTTTGTGTAAAACTGCTAGCTTTTGTTGCAGTTCGTTATGCATTATTTTCGTTTTACTTCCTTGTGTACTACTACCTTGCGACAACGCGAACAAAACTTGTTGAGGGCAAGTTTTTCAGGGTTTTTCTTGACGTTTTTAAACGTTAGATAGTTAATTTCACGACAATCATTACATCCTAGCCGTGTGCTTCTCTTAACAGCCATAAAGTATATAGATTCTAATTATTTATTTTTATCGAGTAATAAACTGATCGAAAACGCATCTATTCCGGTATGCGCAATAATGGCACTTGTGATAAACGAGCTTTCGATTTCACGTTGTAAAGTGATTTTGTTAGTGTGGAGAAAATCCAAAATTAAAGCTTTAACTTCCTTGGCCTTAACTGGGGTAAAAGCATTGCAAAAACCAATCCGTTTGATCTTAAAGTTATCACCAAATTTTGATTTGACAAAGGTAAAGATTTTCTCCAATGCTTGGACAAAGGTAAAGGCCTTGCCGCTCAAAGTATTTACCCCTTTATCAAAGAAGATAATCGGTTTAACCCGTAAAAGTGTGGCAATAATTTTCTTTAATCCAGAAATGCGCCCACCTTTACGTAGCTGCACTAAGTTTTTTACAGTCACAGCTGTAAAAATACTTTGTTTGTGCTGATCAACCACTTCAGCAATTGCTTTAGTTGGGCAGTTAGTGTCAGTTAATGCCTTCACCTCTTGTACTAACCATTTCAGGGAAATGGCAATGTCACTTGTTTCCAAGACCACAAACTCTTTTTTGGGCGTACTGAGTGGCTTAACCGCTTGGACAATCATGTCATACGTACCACTCAAACCCTTACTGAGAGGTAAAAAAACAAAGCGATCGTACTTGTCTTTAATGGCTTCAATTAACTCAATTAAGTCAGCTTGGCGTGGTAGTGATGTGGAAATGTTTAACCCTTGGGGGTGTTCCTTCAACAACTGGTATACTCGATCATAGTCAATGTCCTTACCATCGCGGTAAGTGTCTTGACCGTTAATAATGACCTGTAAAGGTAACACGTAAACGTCTTGGATCTCTCCTTCCTTAATTGAGGCAGTCGAGTCAGTAATTATCGCGGTCTTCATGCGCTCTATTTGTCCTTATTTCGCTTTATATTATCTAGAAACTCCCCAATGTAATCAAAAAAAAGTTTGGGTGCATAGTAGTGGGGTGAATGTCCTACACCATCAATAATTTTGAAAATGATCGTTTCGCTGTGTTTAGCTAAGTATTCCACAGAAGCCTTTGTGGGGGTGACAATATCATTGGAACCGAGAACCACTAAGGTGGGTTTGTCCTTAATTGCGTTGTAACCCGCTTCGAGATAATCATTACCATACTTGGCGTTTTGCACCATGTCACTGTAAAGTGTTTTAAAGTTGACTCGCTTCTTAAAAGCACCAACAAAGAAGTTAACAATCGACTTCTTATCATCGGTGTGTTCTATGAAGTCCTGCTGGTTTTTAGGACTCCTGGTAAACAAGGTATCCAAGATCCGTTTTTTACTTACAACAAACGATGTTTGATTCATCGGTGCTACCAACACTAAGGCCTTAATGCGTTCACGCAATACAGCATTGACCAACACCGCAATCGCCCCGCCCATACTGTGCCCTACCAATACCACCTTTTTCAAGCGCTTTTGGATGATAAAGTCACACACCAATTCAACATAGTGTTTCAACTTCAATTCATCAACACTGTTACTCTGATTATTGCCGTGTCCCGGAAAATTAAAGGCAAAGAACGACCAGCGCTTTTTTTCAAACAGTTTAAACACGTGTTTAAAGCTGGAATACTCACTGCCAAAACCATGGAGAAAGATAAAGTTATGGCGCTTACGGTGTGGTTTAAAGGCAAAGATTGATTGAAAGGGATCGCTCTTACTGCTTGCCATTGATCTTAGCTAGGCGTTCAGCGTACGCTTGGATCCGTTCCTTTTTGGATAACTTGCCCAAAGCATCTTCCTTGTTTTCGATCACAATATCACGTTTTTTTAGCCGTGGTTTCTTTTCGGGTAGCACTGGAATTTCCTTTTCCACAATAATGACCTCTTTAACAGTTTCGTCTTGCTTTGTTTTAATTTCGTGGGTTTGACTCGAGTTGAGTTCGTTGAAGTATTGCAACTCGTGTACTAAGGCTAAGTTTTGTTGCAGTAAGCGTTGTTTTTCTAGCTGCACTTGGTTAAATTGTTCTAACAATTCATCGCTTACCGGTGGAGTTGCTTGGAGGTTATTGTAAGCAGCTTGGAGTTGGGTTAGCTCTGCATGGGTTTGACTTAAACTTTGGTGTGTGTTGTGTAACTTTTGCGCTAACTGTTGTTTTTCCAAATTGACCTGGTTTAAAACTTGCAACTCTTGCTTGAGCTTGTTGTGATTACTTTGTAACAACTCGTAAGCGGAGAGCAAGTCCTGGTACTGGGCGTTGGCCTCATTAAGGTTAGTAATTTGAATTTCGTTTAATTCTGCTTTGACCTTGTTGTAACTCTCTTGCAGTAACTCATGCTCCGCTTGGATTTGGGCTAAATTACTGTGTTCATGTTGCATTTGCTCTTTTTGTTGAGCATATTTGTCTTGCAACAAGTTATGCTCTGTATTGAGGTTCTCCCAGTTAGTCTTTACCCGGTTTAAATCATGCAACTCATTTTGAATGTTACTGCATTTATCAGTTAATTCCTCGTTGTTTTGCTTAAAGAGTGCTAACTGTTGGTGGGCAGCATCAAGTTCATTTTGTGCTTGATGGAATTGTTGCTGGGTGTGATTAGCTTCATCTTGCAATTTGTTGTAACTCTCTTGCAACAGCTCGTGCTGCGTTTGGAGCTGGGTTAAGTTTTCATCACTCTGTTGCAAGTGGTTTAACTGGGTTTCGCTAGCCATTAGTTTGTCAGTTAGGCTTTGGTTTTGTTGTTTGAAGGTAGCTAACTGTTCTTGGGTTAAGCTAAGGTCAAACTGGGTTTGTTCTAGCTGTTGCTTTGTGAGTGTTAGCGTTTTGCTGTCAAACGCAGTTTTTTCCTTGTGGGTAAATTCTAAAACATTTAGTTGGTTTTGTAGTTCACTGTTAGTAATACCCAAGGCATGGACGTTTTCCTCAGCTGCTTGTAAGCTTGCAAGTAAACTGCTGTTTTTTTGTTCTAGTACCACTTTTTGCTGTTCAAAACCTTGTGCTGCGAGACCTAACTCAAAGTTAGTGGCCTTAACTTGGTCTAACTCTTTTACTAAAGCTTCGTTGTGGCGTTGTAATTGTGCTAACTGTTCCTGGTGGTGTTGGTGTGCTAGGTGTTGTTCGTGTTCCAATTCCTTTAACTTAGCATCACTGTCAGCACGCATTAACTCATACTGCTTTAAAAGATTACGGTACTTGGTGTTTTCATCGTTTAACTTAGTTTCACGTAGCTGTTTAATTTCGGTTTTGAGGAAGTTGTTTTCACGCTCAATTAGGCTTAAGCGTTGGTTAGTTTGCTTTAAGCTAATTACTTCCGTTTCCAAACTGTTAATTTCACTGCGTAACGACGGAATTAAGGCCAGTTGTTCCTGGTGTTTTTTGGAATCAAACTTGTACTGTTCGATTTGGAGTGTTAAGCTGTGTTTGTCTTGTTGGAGTTGTTCAAGCTGTTTTTCCAAGACAAAGTTTAACTGGTTAGCTTCCGGTAGGTTGTTTTGTAAATGCTGTTTAAGCGTACGGTTTTCCTCTTCCAAACGTGCTAAGAGTAAGCTGTTGTTTTGGCTCTTAAACAGCTTTTGTTGCAGTTCATTCAACTCCTGAGTGAGGTTAGTATTTTGGTTTTGCAACAGTTCAATCCGTTCCTGTAACACCGGACTTTGGTCGTACTGAAGTGGCTTTGCTAACTGCTCTTCTAGCTGGATTTGTAAAGCAGCGTTGTGCTTCAAGGCCTTATCTAACTGTTCTTGGAGCAGGGTCATTTCAAAACTCACGGTAGGTTGTACTGGCACTACATCTTGAACAGGCAGTAGCTTGTATTCTTCCACACCCATGTAAGTTACAGGAGGTGTTTCTAGTTGAGCTTGGCGTAGTTCAATTTGGGCTTGCAATTGTTGTAACTGCACTTGTTTGGTGGCAATTTCCTTGTCTAAAACGGCACTTTGATGAGCGCTGTCTTCAGCGGGTTTTATAACCACTGTTTGGCTCAAAGCATTAGTTAAATTAGCCTTGTCCACCGTTGTAATCGAGAAGCTATGTTCAGCTGCAGGTGATGTCACAACAACAGGTGTAACTGGTGCAGACTCGTTGACTAATGTATGGGTGAGTGGTACTGTTACTAATTCATCGACTAACTGGGTTTGGTCAAGTGCAAAGTTAGTTGGTGCTGCTTTTGGTTGTAACGGTGCTGGAGTTACAGTGTGAGTTAAAACTGGCACTTCCACTAGTTCGTCAAGCAGTTGTGTTTGGTCTAACGGAACAGTTGTGGTAGTTGGTGCAACTGTGTCAAATACATGAGGTACCTTCATTTCCGGGTACACCAAAATGGCATTACTGTCAATCGGACTGCTAAAGGTCAAGCTAACAGTAGGATCAAGTGCTGCTTGGGGTAAAGCAGTTTGGGCTTGAACATCCTGTTCCACCATTTCGACATAGTCATCCAACAACATAGTTTCACTAACAGCTTTTGTGGCTGCTGGTTCTGTTGCCACACTAAACTGATGGGTATGGTTTGATGAAACTTGTTCTGATGCGTTTTTGATTTGTTGGGTTAGTTCAATGTATTCGTTGACAAGCACACTTTCATCGACTTGTTGGGTTGAAGGAGTATTAAAGACCTCTTCAAACAGTACTTCCTTAGTGGCAAAGGATATAGGTATTGGTGTTGTTTCTAACTCTTTGGTGTGCACGTATTCATCGACTAGTTGAGTTTCATCTAACTGGGGCTTGGTCGTTTGTTCGGTAATACTAGAGGGGGTAATTTCACAAAAACCAGCCTTAATTTCCTTTGTTAGTACTTCCTGGCCTTCTTGTTCTAAAAACTCCATTGTTAGATCAACGGTTTGCGGTTTATGAACTTGGAGTTGGTTAGCGAAGTGGTTTAACACATCAACATGGGGTTGGCTTTTGACCACACCCATATCTACCATAAAGTGAAAAAAATTGCGAATTGATTGTTCTTTCTCTAACTGATCTAATTCGCCAAAGAAACTACGTTCTACAATGTTACGTTCTAGGGGAACTGTTATTGTTTCCCTTGTTGGTTCTAGGGTAATGTTACGGGTTTTGGGCATGAGTTGCTTGAGCAAACTTTGTTCGTTTAAGTTTTCGTCAAAATCGTGGATACCTAGCTCTTTTTCTGCTGTCATGAATGGCACAGACCTCATCTAGTTAAGTATATTATCTTAGGTTTTTGCGACACCTCTTATCCGTGCATTTTTGGACTTGAAATAAAGAGTAATAGGTACAGCACTAAAACCAAAGCTTTCCCTAATCTTGTTCTCTAAAAAGCGCGCATAGGAAAAGTGGACAAACTTAGGGTCATTACAGAACAGTACAAAGTGCGGTGTTTGACTGTGAGTTTGCACCGCATAAGTTACCTGTAACCTCCCACCCTTAAACAGGGGTGCTTGGTTAAACAGTTGTGCTTGTTGGATGACATCGTTTAACAGGGAAGTTGAAATCTTTTTGGTGAGCTGTTCCTGGATAATTTGGAGCTGTTCAAAGATGGTATGTAAGCGCTTGTTGTTTTTGACACTAATAAAAACAATGGGAGCAAAGCTGAGGTGTTGAAACTGACTTTGTAACTGCTTTTTAAACTTAGCCATCGTGTTGCTGTCCTTCTGTACTAAGTCTCATTTGTTGACCAGAATAATCACCGGAATTAAAGCGGCTTGGGCTAATCCACCGATAATCTCGTCCTGTTCACTCAACGGTTTGGAACCATCAACCATTAACAGAATAATGTTGCTCCTAGCGATTGCTAACTGGGTTTTCCCATACGAGGCCGCTTCAATTCCGGGGGCTATTTTCCCCTTACGGCGAATCCCAGCCGTATCAATTAAAAGGTAGTTTTGCCCATTAACCCTTAAGGGAATATCAATTGCATCACGGGTAGTACCACTCTCATCGGACACTAACACCCGGTTTTGCTGCACCAGTTGGTTAATTAAAGAACTCTTGCCAACATTGGGTTTACCAATTACACAAAAGCGCACTTCGGGATTGGTTTCACTACTACCATGGTAAGGGGGGAGTTGGTTGTCCTTAACTAGTCGATCCATTAGGTTACCAATGCCAATGCCATGGCTAGCACTCACACAAACGGGTTGGCCAAACCCAAAGCTGTAGAACTGTTGTAGGTTGGGTTCATAAGCATCTGGTTTGAGGTTTTCTGATTTATTGACGACCAACAAAATCGGTTTGTCCTTAATCTTTTTCAATACCTTAGCTACATAAAAGTCATCACTGCTAATCTGTTCTTGGTAAGACACCAAGAAGATAATCGCATTAGCTTGACTTAAGGCTGCTCGAACTTGGACTTCAATTTGTTGTTGGAGCGGGGTCTTTTGGGAAATTAATCCCCCAGTATCAATAAAGGCAATTTGCCGCGTTAACCATTCACCAACACCATAAATGCGGTCCCTTGTGGTATTGGGCGTATCGGAAATAATCGCGTAGGGTTTTTGGATTAAGCGATTGAACAAACTCGATTTGCCAACGTTAGGTCGGCCGATAATGGCAACAGTAAACACCTTAAAGTTTAAAAACTGTACGCGCTTGTTGCACCATTAACTCGACTACTTGCTGAAAAGTAAGGTTAGAACTGTCGATGTAAATCGCATCGGGGGCTTGCACTAAGGGATCAGCAGTTCTGGAAGTATCCACCGCATCACGGTGCTTAATTTCCGCTACTAGTTCCTGAAAGGCCTTGTCACTGTCATATGCTATGCCAAGATCTTGGGCGCGTCGTTGCGCTCTTATTTCCGGTTTAGCATCCAAGAAGTACTTTAACTGCGCGTCCTTCAATACAACGGTACCAATGTCGCGTCCATCCATCACAATGTTGGTGTTTTTAGCTAACGCTTGTTGGCGCAATACCGCCATCTTACGCACTTCGGGATTGGTTGCTAAATTGCTCGCTAGTTGGGCAATTTCCTGGTTTAGGATAACCTCTGAGATATCAGCGTTGTTATAAAACACCTTTTCGTGTTCAAAGCGCCAGTTAATTTGGTTAATCACCTTTAACAAAAGGTTGACATCCAAGCGGTTGACCTGCATCACGTAAGCGAAGGCCCGGTACATTTTGCCAGTGGAAAAGTACTCAAAACCGAGCTGTCGGGCCACTGTTTTAGCAACACTAGATTTACCAGAACTACTAGGACCGTCAATGGCAATTTGAAAATACATAAATTTAACTTAATGGGTGTAAGCAATTAAAGGGAAGTGAATAAGAGGGTTAGTAAAACAACCAAACCCACTAAACTACTAATAAAGAATACTAATAAACTGAGCAAAATCCGCTTAAATAACGGGTTGTAACTGCTCGCTAAGGGCTGGCTTAAATCAGTGGTGGTAAAGCTCACTTTTTCGTTAGTGAGTGCCATTAGTGCCTTATTATAGTTCCCCAATTGCACTACGAAAGTTTGCGTTTGCTGAATAAACTGTTGTTCGTTGTCCTCAAAAAAGCGCTTTAGGTTATCTATTTTATCCATCCCCCGGTCATCAGTAATCCAACTTTCATAACCAGTCGCGCTATCGTGGTGTTTAATTTGTAACCACAAGGCATCAGCTTGGGTGGCGATGGCTTGAAAATCCGATGTTTGGAAGTTGTTAATGAGAGTTTGCAGTGATTGTAAGACCTGATTGGGTTCACTTTTGCGTTTCACCGGATTAGCACTCAATTCACTAAAAGGCTGGTAGTGCGACAGAATTTTCGGGCTCAATTCGTCTAGTTCTACTTTTCAACTAGCGACCTGTTTTTGCGCTTTCAGTAACGCTTTTTGAACAGTCGTATTATTGTCATCGTTCTGGCGGTAAAAGCGAAACTTCTCTAAGCGTCCCATTGCTACAGTGCATTATGCACTACTGTTTGGATGTCATCATCCTCTTCACACGCATTTAAAAAGCGGTTTAAGAGGGTGTGTTGTTCGGCGTTAAGTTCCACCGTTAACAGCGGAATTAAGCGCAACTCACTCGCTTCAATCGTAAAACTGTTTTTAACGAGTAAATCCTTCAACGCAAAGTAACTCGGTGAATCGACCACTACTTCAAAGCTGTCATCATCTTCATTGAGATCTAACAAATTAATGTTGTTACTAATTAACAGTTCAATTAACTCGTCTTGGGTAAAGTTATTGTGTTTAACTAGCGCAATGCCCTTTTCCTCAAAGTTAATTTTAACACTGTTGGGCTTGGCTAGCTGTGCCTTTAATTTAGCAATGTAACCGTTTAAACTACTCAACAACCGGTTCGGATTATCGGTTAGACCAAAGACAATGATGCCAACACCATTGGGACCAAAGATCTCATAGCAAAACTCGCTTAGTTTTGTGGGATCCTTTTCACTGCCATGGATGTTGCGTTTAATTGAATCCGCACTTAAACCATGAGCTAACGCTAGTTCGACTGCCACCTTTAGCTGGGGGTTGGCATCAATGTTACTGCCACCTTTTTTGACAGCAGCAGCGATCCGCTTCGCTAACTTTTGTAGTTGCTTCGCGTTGGATTGTTGTTTTTTGTTTGTTTGACTGGCAATTAAATGCTTCCGCGGCATGAATTAAAACTCATTAGCAATTGCGTCCACTTGTTTAGCGAACATGTCCACTACTTCACTCTTACTTTTGTCCTTGTAAAGTAGCTTGGTACCATCAATGATTACACTAGCAAAACTAGCGACTCCAGCAAAACCAAACAGACCGCGTAGACTGTTGACATGATCGCCCCATGGGTATCAACCTAACGGTGCGCCTTGGGAACCCAAAACGACTACCTTGAGGTTACCCAACATTCCTGATGATGCGCCCTTAGTGACATACTTATCCTTAAAGGTTTTGTTTGCCACAAAGACATGGTCAATAAAGTTCTTTAACGTTGCGGGGTAGTTAAAGTTGGTCATTGGCGCCAAGATAATAACACCGTGCGCTGCTTTGAGCGCATCAATGTACTTATCGGAGTTTTCTACAGAAAAGAAAGTTCCAGCGTTTTGGGTGTTATAGGAAATTTGTCCCACTGGTAGTTCGTTGAGGTTCCAAGTGGAGAGCTCTACGTCCTTGTTTTTAGCTTGAAAGGTAGCTAAAAAGCGTTCTAACAATAAATGGGTGTAAGAACCACTAGGTGATACAGAAGCATCAATAATAATTACCTTCTTCATACTGAACTAATTGTTATTGTTTAAATTCTACTCAGCTTTAGTTAATTTAGCGATTAAATGATTGACTTCCGCTAATTTTTTTTGATATTCCTCTAACTTTAAAAGCTCCGCCTTAACTTTGTGCGGTGGTGCTTTTTTTAGAAAGGCAGGGTTAGTGTTAATCGCTTGACTACGTTGGACTTCCGCTAAATAAAAGCTCCGTTGTTTTTCTAAGTGGGCTAAGTTGTTGGCTGCATTGTCAACGATTTTGATTTGGAAGCCTGGAGTTTTGTTAACTTCTATCCTAAGATCGACCCAGTTAAAGTGGAAGTAGTTTTGCACTACAGGAGCAGCTTTACCACTTAAAACAACGGTTAAATTCTGGTTAGCTACTAGTTGGTTGTTGGCCTTGTAGTGGCGCAAGCTGGACACGGCTTCAACAACATCAGCAAACAGTTTCGGTGGTTTAACTGCTTTGGCAGTTGGCCAACTTGCTTGCATGACACTGCCACTGTGAAACTGTTGGTAGATCCGTTCCGCCAAGAAGGGAGTAACGGTACTTAGCATAACGACCGCCGTACTTAAAACAGTTTTAGCAGTTGTGTACAGCTGGGGTAAAAGTGCCGTGTCCTCTTGCTTAATCGTTTCAATGAAGGTATTGCAAAAATCATTTCAAATAAAGTTGACTAACGCTTGGTTGGCCAAAGCTAACTGGTACTTGTCTAATAACTTGGTGGTTTGGACAATGAGTTGTTTCAATTTAGCCCAGATCCAGCGCTCACTCAATGTAGAGGGTAAGTGAGTACTTGGTACAGTTTTGGCTTGTTCTCCTAACTGCAACACGTACTTGGTCACATTCCACAACTTGTTCAAGAAGTTAGCGGCACTCTTTAACTTCTGTTCGTTGAAGATTAAATCATCACCCGGAGTGTGATTGGAAGTTAAGAATAAGCGCGTGGCATCGGCCCCATAATCACGGATTAAGTCCATTGGGTTAATCCCGTTGTTTAGGGACTTGGACATTTTGCGGTTTTGGGCATCGCGGACTAGACCGTGAATTAACACTTGTTTAAAAGGTAACTGCTTGGTTTGAAAGTACGAGTTAAAGGTCATCCGTAAGACCCAAAAGAAGAGGATGTCATACCCGGTTACCAAAAGATCAGTTGGCACAAAGTGCTTGTTTTTGTGTCAGTCTAAACAAATTAACGGTCACAGTGATGAGGAAAATCAGGTATCGAGTACATCGGTGGACTGGGTGTAGTTTTGCTTATCAGTTGGTGCAGTACTGCCCACATGCAACGCACCACTTTGTTTATGTGTTCACACCGGAATAGGGTGACCCCAAATTAATTGGCGCGATATGCACCACGGTTTGAGCTGACTCAACCACCTTGTTACCTGTTTGTTAAAACGCTTGGGAATTAACTCGGGGTATTTCTTAATGGCGAGTGCTTTTTTGAGTTTCGGTAAATCGACAAACCACTGCTTGGATAATAACGGTTCTACGACCGTGTCACTGCGCTCGGAAAAACCAACGTTACTGGTTAGTGGCATAGTTTTCACTAGTAGTTTTTGCGTTTGTAAAAACTCGACTACCTGCTGACGTGCTGCACTTACGGTTAAACCAGTAAATTGCTTAGCGTGCTCATTTAACAAACCATTGTGGTCAATGCAACTTACAAACGGTAAACGGTACTTTTCGTTTAAGGCAAAGTCGTTAAAGTCATGGGCGGGGGTGCACTTCAACACACCGGTGCCAAACTTCTTGTCGACATAACTATCGGTGAACACTGGCATTAACGCACCAGTGAGCGGATTAACTACCTTTTGTTTGACAAAGCTGTGGTAGTGCTTGTCTTTGGGATGGACAAACAAACACACATCAACAAAGATCGTTTCTGGTCTAGTAGTTGCCACCACTAAACGTTTTTTGGGATTATTAGCTAACTTATAAGCAATGTAATACAGCTGTTGGTCAACTGGTTTATTAATGACTTCAATGTTCGAGATGGCAGTGTTTAGCTTGGTGTCCCAGTTAACCAAGGTTTCAGCTTGGTAGATAAAGCCCTGTTCAAACAACTGGATGAAACTATCGTTGACAATTTGACTCGCTTTTTTGGACAGGGTAAAGTTAACCTGGTTTCAGTTTAAGGATGCCCCTAAACTTTTGAGCTGACTTTGAATGGTGTCCCCTTGTGTTAAGGCCCAATCCATAATCATTTTGACCTTTTGTTTACGCGGCGCTTGGAAGTACTCGGGATTAGTTTCCCGTGCTAACTTTTCGTATTTGGTTTGCGTAGCAATCCCCGCATGGTCAAATCCGGGAATCCAGTTCACGCCATAACCCCGCAGTCGCTTAAAGCGCATAATTTGGTCAGTGATACTCACTTCAAAGGCATGACCAATGTGCAGCGTACCCGTTAAGTTTGGTGGGGGGAGAATGGCAGTGAAGGGTACCTTGTTTTTTTGTGGTTTAAAAAAACTAGCACTGCTTCAACTATCATAAAGACCAGTGCTCACTGTTTTGAAGTCGTATTGACCTTGAAAACTAAATTGCTTGTCCATCTGCTTTAAATAACTCAAAGATTTGTTTGTGCATTACTTGGAGGTTATACCCGGTTTTGGCACTAACAAATAAACACTTATTAGGATTGACTTTTAAAAAGTGTGCCAGTTTATTCTGGGTATGAAAGCGCTCGCTTTGGTTGAGCTTATCAAACTTATTGGCTATCACCAAAAAATTTAACCCCGTTTGGAGAATGGTTTTGACCACTTCCTGGTCCTGAACGGTCACTACACCGCTGTCAATCACTAGTACCACACCAGTTAAGTTTTGGCGAAAATTGAGGAACTGCGTTAATAAATTAGTAATTGCTTCCTTTTGCACTTTACTCAGTTTAGCAAAGCCATAACCTGGTAAATCGACAAAGCGTTTACGGTTGTATTCAAAGAAGTTTAACAGTTGTGTACGTCCCGGCGTCGCGGAAGTTTTAGCTAGCTTTTTTTGAAAAAAGGCATTAATGAGGCTTGATTTGCCGACATTACTCCGCCCCATAAAACAGACTTCCTTAACACTGTCTTGGGGACAACTCTCTAAGTCACTTGCACTTTTTAAAAAACGTGCCTCCATTTATTAATTACTTCTGGTTTTTAATGGAGCGCATAATTTCCTTGACTTGTGTTTCAGAAAGCTTACGACCCATTTTGGCATACATCGCCTTAATTTGGTTTTCGGTGATTGGTGGGTTATCACGAATCTGTTTTTTAAAGATTTTGATAGAAATAAAATAGCCAATAATGACACCGACTAACAGGGAAAGGGGAATTCCCAAACCTAAGGCAAGCGCAAGATCATTCATATCTATATCTTAATCTTAATCCTCTCTAGAATCTTATAAAATCAGAATAGCGTAATTAATGAACAAGCTAATTAGCATTCTGGTTCCTTGTTACCAATCCCAGCCCTTTCTCGATCGCTTTTTCAAATCACTTTTAAAGCAAGACTGGAACGGTGTTAAAGTAATCTTCTTTAATGATAACAAGCCAGATCCAACATACGAGATCCTAAAACAATTTCAACAAGCACATCCACAACTAGCGATTGAAGTACATTGTGGTGAGAAAAATGTCGGTGTAGGCGGTTCACGTGATCAACTAATAAATTATGTAGACACACCCTACTTTTACTTTGTTGATCCTGATGATGAATTTAGTGATCCCAACTGTTTTAAGGCAATAGTGGAAACAATTCAAGGAGAAAACTTTGATATTGCAGTGTTAAACAGTATTGTTTACCTCCAAATGTTGAAGAATGACTTTTTAATTAAACACATTCCGCTCAAAAACATCTTTCAGGGCAAGGTAAAACTCAATCCTGATAACACCGTAAATCATTTGCACTATATCCAGAATAACGACCAGTACATTTGGAACATTGTCATCAACACCGCCTTTTTCAAAGCATTGGATTTGCAGTTTGTTAACCGCTTTATTGAAGACATTGCGGTGTGGTTTCCGATTATGTTTAAAGCCCAAAAAGTGCTGTGAATTGATGTAAATGGGGTTAACTATTACTTGCGACCGAACAGTGCTTCCACCCAGAAAAACAGCATTAAATTACTCTCCTTTATTGAGGCTTACGAACGGTTGTATTTCCACTTAAAGAAGGTGGGTAAGCTAGCTGATTTTATTGACCCTAACAATAAGATTGAAAGTCGTTTTTGACGGCGTCAAGCCTTCATTTGGTTTAGTTTTATTAATGTTTCATGGATGAAAGCGGAATTTGAACAGACCAAAAGTGTGTTGCAAAAACTGTTTGACTTCATGGAAGCCAACGGCATCTACGACAGGGTATTTACTAATAAGCACCACGGTATTTACTTATTGTGAGTTAACCGCTTAAAACACTTTAAGAAGTTAGTGCAAGCACAACCACATCTATAAGTTTTGTGTTCTAACAAGCTAAAGCTTCGAGTAGCTTTTTAAAAAGTTGTTCTCTTTCTAGAGTTTGTTTTCCATCTTGGGCTTGTACAACCATTTCCTTTTGATTAGCGTGAATCTTTATGTAAAGTGTGCGTTTATTATCTTCATTGGCTTCGTTAAAAGCTACAAATGAAATTAGCTCACTTTGATTGAGTGAATTCATTGCGTGATCAATAAAAGGTAGTTTGATAATTTTTTTATTTTTCATAATTAATATTTGTTTTTTGGAGATTGAAAAAATAGCTACCACTTGCAACTCGGTTGCCAACAGGGATCAGACGTTTTGCTTATCAATTAAAAAAATAAAAGATTTGCATTTTGCTCCTCCACTAAATAAATTGAACTGAGACTTTATATGTGACAACAAAATTACCAAAAATTTTTTTGAATATTTTTTAGATAAATAAAAATACCAACAACTGACATATAAAAATCAACTGCTGGTACTTTTATTTTGGGTTGAATCTACTTAGACTCCAAACGATCCAAACGTTTGTTAATTACTTGAAGAGTTTCAAGAATTAACTTAAGCGTTTCCCCTTGGGTTTGAATTTGTTCGCCCTGCTTTTCAACAGTTTCAGTGAGCTTATTTATTTGTTCACCCTGAAGAATTGAAACTTTGATTAGCTGACTAATTTGTTCACCTTGAGAAACCAAAGTGTTCTCAATTTTTATCAGCCGCTGATTGTTGGAATTTTTGAAATCATTAAACTGCTTTTGGGTAACGTAACCACTTTTTCGTTTTTCCATATGGTTTAAATTGCCATAAAAGCCCATGTCATAGCTGCCATCAGGTAGATCTTCGATTCAGGCTTTTGATGTTTTAATCTTATCTTTTTTCAAATTTCTTTTAAACAAAGTAATTGAAATTTCACGTTTAATGATCAAATCCTCCTTTCTTTACAAGATTTAAAAAACTACAACGTGCAACAATGTTGCCAGAGGATATCAACTGCTTGGTTGATCCAGTAATTTTCTTGAAATACTTCATAACGATCATTTCACTAAATAAATTGAGTAGTTGATTTGGAAGTAACAAGTAGAATTTCTGAAATTTTTGAAGAGTCAAAAAATCTATTCATGAAATTAATTGAATGAAATTCAGGTAACAAGGCCTTTTCAAACTAAAAAAGCGCTTTTGCCGCCCTACTAATTAATCAATTGCGTAAATCAAATTGAAACGCCAAATTTATTTAACTCACCTCCCCCAAATCTTTCTTAAACTCACTTAACTTCCCCGCAGCAGCCAAATAATCCTTGTACTCGGATTGGATGTCGGTTAAGGCCTTTTTAAAGGTGGGGTTATCAGTGAAGGGTTTGGCCAAATCCGCACTTGATTTCAACAGCGCTTTGGCTAACTTAACCACTTCCGATTCTTGGGTATCAGTCGATTGCTGTCTGCTTTGCTGGGTAGTTTGAACGCTAACAGAAGCTGCTCGGCTACTCCCCTGCACACTGCCCTTAACTTGGGTCCCCTGACCCGAACCCATCATCGCCACCAAAGCACCGTACAACTTCGCGGTTAACCCCGTTTGTGCTCACGTTTGGAACAGGCTGCCAGATTCCAGTAAGTTCAAACTAAACTCGACGCCGTTTTCCTTAAAGTTATTACTCTTAGCTTGTAAATAGCGGTACAGCAAGATGTCGGCTTGTTTGGTGAAATCGCGTTTGTTGTTCGCCAAAAAATAATCACCACCGTCAATTGCCAGGGCGTATGTACCATCGTCACCACGGGCGTAAACAGGGGTTTGGAGTGTGGAGGTGGTTTGTTGAACTTGTTGCTGGGTGTGGGTAAGGGCGGCACTCCTTGGGAGCGGAATTAAAACAGAGTTGCCCCCGGAGCTACCACCAACATTGGTACCACTACTAGAATCTGCCCCAGTGGAAGCTGCCGCCATTGCTTGACTACTCGTCAACCGCACCGCGTCTTGGAGGTAGTGGTGTTTATCATTGGAATTATTACTACCCCCATTAGTTTGCCCGCCGCTCAGTTTGCGCTGGGTTGTGTAAAACGCATCGGTTTGATTATTCTGCTTCACCTTGACAAACGCCGGGTCGACCTTAAACCCGGGGGTTTGGTCGGTCATGGCAATTTTCGCACGCGAAACTATGGATCTAATCGCCACGGCATCCTTCCCCTCCATAAAAAAACTATCCAGCGGATTTTGGTTGGACTTATTCGAATCTACCTTGACCTCGGGCAACGAACTCGTCTTACTCCCACCCACCAGGTACTCGTACTGGTCAATAATGGCCGTAGCTAACCCCACGCCCTTAAAGGCCCCGCGCAGGTTGGACTTGGTCACCAAGAGCTTATTGGCAGCGCCATTGCTGTTTCACTTGGTTTGCCAGTCAAACAACCCGGTGCGGGAGGATGTATTGGATTGTTGGGTTTGACCATTCTTCTGCTCCGGAAACACCGCCTTAATGAAATCCCCGAAGTTTTGGGCACTGTTGGGGGTGGGGTCGAAGGTGGTTTTACCTTGGCTTTGTCCATTATTGGGCTCGCGAAACGCGGGGAAGGCATAGACCAACTGTAAGGCACTACCAATCGCGGCTTGGTCGTATAAGCTGTCGAGCCCGTCCTTGGGTGCTTGGTACGCGAAGAACGCTTGGGTGAGGGGAAACAGATAGAGCAAAAGCTGTAAGCCACTTTATAATTAAGTAAATTATAGCAACGCTGTCAAAGCGTACTTTTACCATTGATTATTTCCTTAAAAAGACTTGTTTAAATGACCAAAACTAAGTTTAATCCGTACCAATTTCGCAAACAATTTAAGTGGTTTAAAAACAACCCCCAATGGGTTAACTTTGACAACGCTGCTACCTCAATTGCGCTAGACACCGTTAGTCAAGCTTGCAAGGAATACTATGAGCTTTTTAGTGTCAACCCCCACAATAAAACCCCTGATCTCAACAACCAGATTATTGCCATTATTGCTGAAACCCGTCAACTAGTAGCGGACTGGTTTAATGTCACTGCACCTGAAATTATCTTTACAAGTTCAGCCACGGAGTCCATTAATTTGTTTGCCCATGGCTTAAAGCCCTGGATTAAACCCGGTGATGAAATTGTCTTAAAGGGTGATGAACACTCGGCTAACGTTTTGCCCTGAGTGGCACTAGCTAAACAAACAAAGGCCAGACTAGTATGGGTCGAAAAGCAAGCTAACCAATCCTTAGAAGACACTTTTAAGTCATTGATTAACCCGAAAACTAAAGTAGTAGCCATTACCGCTACTTCCAATTTGTTTGGCAATTCAATTGACTTTGCTCAAATTGCCGATTACCTCAAACAAGTCAATCCTAAAGCTTTTGTAGCCGTGGATGCAGTGCAAACGGTGCAGCACAAGCAAATTGACATTGCCAAAACCCAGATCGACTTCCTAGCCTTTTCTACTCATAAGTTTTACGGTCCCACTGGACTGGGGGTAGCTTACATTAAAAAAAGCTTACAACCCCAATTACAACCCCTCAAACTCGGGGGTGATATCTTTACCCAGATTGACCCGGACAATACAATTCACTTTAAAAGTACACCCCTAAAGTTTGAAGCCGGTACCCCCAACATTATGGCCATCTATGCCTTAAACAAGCTGTTGCGCTTCTTTAAACAAAAGTTTAATTTTGCCCAAATGATGGCTTATGGTCACCAACTTAAACAACAAGCTTACGAACTGTTAAACAGTAATCCGCAAATTGTGCTGGCCAACCACGACCAGGATGTCCCGATTTTTTCCTTTAAACACCGCCAATTGGCTACCATTGATCTAGCCACTTTCCTAAACATCAATAAGATCATGGTACGCCAGGGCTCCATTTGTGTGGGACGTTACCGCAATAAGGATTACTTTGTGAGAGTTTCGTTAATGCACTATAACACAGTGAAGGAGTTGCAGTATTTAGCTAAACTCCTAGCAACTGATACCAAGACAATTATTAAAAACGTAATTAAGTAACTATGGATCGCAAAATAAGGGAGCAAATTATTGCCATTTACAGCAACTTACAACACAAACAGGACTTAACTCAGTTTGCCCATTGTTTAACCACTAAGGCTGATGATAACTGTGAGGACTTCTTTAACTTAGGCTTACAGTTTCAAAACAACCAACTAACAGCACTTGGTTTTAACGGGGAGGGTTGTATTATTTCGACAATTGCTAGTGAACTGACTTTAAGTGCGTTGGAAGGCAAAACAATCAAAGAAGCCATTGTCTTGTTAGAACAGTTTATGGAAGCTGTACAAACTGGTAGCTTAAGCACGCCACTACCCCAAGCTTTACAACTGTTGTGGGACTTTCAGATTGACCAGAAGCGTTTAAACTGCTTACTCCTAACACCACAGAATTTGCTACAGTGGCTTAAAGATTTTTCGCACTAAGCGCTAGCGTTTGCCCTAACGCATCATACAAGCGTTTGTCACCCACCAGGATTTGCTGGTGACTACGTTTGTTTTGCACCTGGTTAGCGCGCACTAAGTCTAAAAAGGCTAACTCTTGCGTACCAGCGTCCAAGGCTTGTTCTACTAGTAAAGCCATAAAGATTTCCTTTTTGAGGCCTAAAGCACTGTTCTTATCAGTTTTTCAACTAGCAGAAATTTTGTTAACGTCCTCGTTGGTAATCTGAATGACATAGCTCAAAGTCTTGACACTCGAGTCCTTGGTGAGTGGTACGGCTTTGGCTTTCTTATAGTCTTCGTTACCTTGGGAACTACTACTTGAATCAGACATCTTCACCTTGGTTTGGCCAACATAACCTTCGAACTTGTTAAAGTAACCATCATCCAGTTTGTCCACTACATCAACCAAGAACTTCTTCTTGTTGTCAAAGCTAATCAACGGTTCCCCACTGGAGTTAAAACGTCCTAAGTTACTGGTATCGACATTAACTGCACGGTGCAACACTTCACCAAAGGCATTGAGCTCGGCATCGGAATTAGTGTTTTGGATTAAGCGTTTCAGGGTTGCTTTATCCTTGTACTGGCTCAAGGCCCCGGATTTGTCACTACTTGTTACTAACTGACCAAAGTTGTTAAAGATTTGTTCCGTGAGTCCGGCACTAGCAGTCGACACACTTTGGGGCGAGTTAATCGTTAAACCGTTAAAGCCAAAGAAGTGTTTTTCGTTAGTGGTACCACCAGTAGACCTCTTTTGACTTTTCGTCTTATCATCAAACGGTTCCGCGGTAAAGTTGTAAGTGCTTTCGCTTTCCAGTAACGAATCGGGTTTAATTGGCGTACTCTTATCGTTAAATCAGTTGGTTTCCGAACCCTGGACATAATCGGGGTTTTGGACAAACTTATTGAAGGGGTTGTCGCTTTGAGTATTTGTTGCCTGAGCTGTCTTTAATACTGCTACTTGCGCTTTTGCTGCGGCTGCTGATGCCGTAGCCGTATCATCATAGCTCACCGTTCACGACACAAAGGCACGGGTACCCACGGTAATCGCTTGTTGGAGCAAGCGCTTATAGTTCTTCGCCCCATCCTTAACTAGTCACATTAAGGTGGACAAGAAGCGCAGGTACTTGTACTGCCCATCACTACTAGATGCTTCCAGTTCACTTTCAAACGCTTTTTTACCGAGTTCCAAGAGGGTTTGATCACCAGATTTTTGCATGCCACTTGAAGCTACAGCATTTGCAACTGCTGCGCTACTTGAAAGTGAAACCATTCCTGATTGAGCTGCTTGTGCTTTTACACTACCCAACTCCTTGTAATAACCAAACTTTTCCTTGGAAGTGAGTCGTTGTCAGGAACGTAAGTACAAGGTTTGTTTAACGAGGTCGTTAAAGTCCTTGTACTCATTGCCTTTAAGTTCTTTCTTTTCAGAATCGTAATTGTTCTTAAAGTCGTTGGTATTAGTAAAGAACCACTCCTTAAAGGAATTGAGAATCTTGGTTCACTTAATCCCAACATCAGACAATAAGGCCAAGATCATGTTGGTCTGGGGCTTATTGTCCTTACTCTTGAGCGAAACAATTTCGGAGAACTGACTACCAATTTGGGGTGTTGCACCTCATTTGGTGATTAAACTAGATGCTTTAGTTTCTACATCACTAGTTAACTGCTTTAACTTTTTGTCGTTGTTTTCCCGAGTCGGTTTGATGTCCGGTTGTTTAACGGTGTTTGTGCTTTTTTGACTATCTGTTGTCTTTAGTGTTACTAAAGCGGCAGATCCTGATTCAGATGCTTTTACTTTTCCATCTGCTGCACTATCTTCCGGAATAAAGAACTTCTCTAAAGCGGGGTAACTACCATCACTAGCGCGTTGGTAAGGGAGCGGTTGGCCCAAGCCAATCGCACTCTTTTGGGCTTGTTTTTCTAGTTGAATGTACTTATCAGCGCGGTCAATAATAGCTTGCTGTAAGCCACTCACCTCCCCCAAATCTTTCTTAAACTCACTTAACTTCTCCGCAGCAGCCAAATAATCCTTGTACTCGGATTGGATGTCGGTTAAGGCCTTTTTAAAGGTGGGGTTATCAGTGAAGGGTTTGGCCAAATCCGCACTTGATTTCAGCAGGGACTTGGCTAACTTAACCACTTCCGATTCTTGGGTATCAGTCGATTGCTGTCTGCTTTGCTGGGTAGTTTGAACGCTAACAGAAGCTGCTCGGCTACTCCCCTGCACACTGCCCTTAACTTGGGTCCCCTGACCCGAACCCATCATCGCCACCAAAGCACCGTACAACTTCGCGGTTAACCCCGTTTGTGCTCACGTTTGGAACAGGCTGCCAGATTCCAGTAAGTTCAAACTAAACTCGACGCCGTTTTCCTTAAAGTTATTACTCTTAGCTTGTAAATAGCGGTACAGCAAGATGTCGGCTTGTTTGGTGAAATCGCGTTTGTTGTTCGCCAAAAAATAATCACCACCGTCAATTGCCAGGGCGTATGTACCATCGTCACCACGGGCGTAAACAGGAGTTTGGAGTGTGGAGGTGGTTTGTTGAACTTGTTGCTGGGTGTGGGTAAGGGCGGCACTCCTTGGGAGCGGAATTAAAACAGAGTTGCCCCCGGAGCTACCACCAACATTGGTACCACTACTAGAATCTGCCCCAGTAGAAGCTGCCGCCATTGCTTGACTACTCGTCAACCGCACCGCGTCTTGGAGGTAGTGGTGTTTACCCTGGGAGTTATTATCCCCACTTTGCCCGCCGCTCAGTTTGCGCTGGTTTTGGTAAAAGGTATCGTTCTGCTGACTCTGCTTCACCTTGACAAACGCCGGGTTGACCTTAAACCCGGGGGTTTGGTCGGTCATGGCAATTTTCGCACGCGAAACTATGGATCTAATCGCCACCGCATCCTTCCCCTCCATAAAAAAACTATCCAGCGGATTTTGGTTGGACTTATTCGAATCTACCTTGACCTCGGGCAACGAACTCGTTTTTTTCCCACCCACCAGGTACTCGTACTGGTCAATAATGGCCGTAGCTAACCCCACGCCCTTAAAGGCCCCGCGCAGGTTGGACTTGGTCACCAAGAGCTTATTGGCAGCGCCATTGGAGTTTCACTTGGTTTGCCAGTCAAACAACCCGGTGCGGGAGGATGTATTGGATTGTTGGGTTTGACCATTCTTCTGCTCCGGAAACACCGCCTTAATGAAATCCCCGAAGTTTTGGGCACTGTTGGGGGTGGGGTCGAAGGTGGTTTTACCTTGGCTTTGTCCATTATTGGGCTCGCGAAACGCGGGGAAGGCATAGCCCAACTGTAAGGCACTACCAATCGCGGCTTGGTCGTATAAGCTGTCGAGCCCGTCCTTGGGTGCTTGGTACGCGAAGAAGGCTTGGGTGAGGGGGAGGGGGTTTTCCTCCACTACTCACTGGGCAAATAGATAAGCCTGGAGGTTAGCAAAGAACTCCCCGTTAGGATCATTTGGTTTGGAATTAACAAACTTGATTTTGTTGCTAGTACTGTCGAGGTTATCTTTGGTAGTGGGTTTAAACTGATCACTACCATCAGTCAACTGCACATTAGCAACAATGTTGCTAAAGATAAGTGATTGGAAGTCGGCTATTAAACGGTCGTATAACTTTAAGGCCTTTCAGGAACTTTCACTCCCACCCCACTGGTCGTATTCCTTTTGGAGCTCTATTGGTCAATTAATCGGTTCGGCCTGTTGTTTTTGGTCCAACAGGTTATCATGTTCATCCTCCACGTTTTCACTAAAGATAGTTAAACGGGTAGCAATGTTGCGATCCTTATTGTTTTGGTACCAACTCTTTAACGCTTCACCAAAGTTAACCCTTAAAATGGTTTCCGTACCATTGTCCTCAGCTAAAGCGGCTTTTAGTACAGCTTCAGGTTTAATGATGTTGTTTTCGGTAAACCCAATCAACTGCTTGGAGCGGTTTTGACGGGGAAACAGATTGGAGGTATTGAGTGCAGAACACGCCGCTAGCACCACCCCTAGGCCAATTCCGGTAAATGTAAACGCAACTAAAGGTCATCGCTTTAACTTATAACCCATAAATTGTGTGTCCGTTAAAGAATAATAGTTTGCTAATTATCTCATGAATGGCACTAATTTAGCCATTGTTTATCCAGCTAATATAAATAGGTGAAGGTGAAAAACTTCAAACCGCTTTTACAATTTTGTGAGTTCTTTTGCCCAATTAAAACATTGGGCAGACCAGCATTAGATTGCTATGGTATATTTAAGCGGGACTTGGGAACCGTTAATACTATCTCATTTTCCTCAAGATACAACTGTTTCATTTTTTCGTAACTTTTATATTTTGTAAACGTTCCTCTTATCATTCATACATGTCTTTATAATTCCCAAGTTCTAAAGAGTCCCTTCGGGGACTTTCTTAATGCTAAAAAAGCAGTTTGATGCTACTTATAGTCGCTATAAGCCATCTAAACTGCTTTCTTAGTTTTGATAGTTATTCCTAATTTGTGTCTATATATTATATATAGTCCTTATTAATAACTATTTTTATATTTACCGGCGCTGTGGGGGTTGGCTAGCTCGCTGGGGGTGGCGGGCTGTGGATTTGCTTAATCGTGAGCTGCTTAATTTGTTCAAACAACTCTGGTTTTTCCGCCAACACGCTAAAGAGCTTCTCTTTGCCAATCGCAATGTTTTGGCTCTTAAACGAATATGCATTGTCACTGCGCACCACCACATTGTGTTTAAGCGCTAACTCAATCACTTCGTGTTCATACACAATACCGCGGTTAAACATAATTTCCAAAAAGGCAACACCAAACGGGCGCGCTACCTTATTCTTGGACACGGTTAACTTACTCTTAATGCCGACATAGTTACCAAAGCGGTCTTTGAGCAGTTCACTGCGTTTGGCCTCCATCCGCATCGAAGCGTAGAACTTCAACGCCCGACCCCCAGTAGTTACTTCCCCATTACCAAACATGACTCCGGGTTTTTCCCGGAGTTGGTTAATAAACAGTAAGCAGGTGTCAGATTCAGGTAAGAGTGACTGCACACGGCGTAGTCCCTTAGACATCATCCGCGCATGGAGTCCTATTGTCTGGTCATCCATGTTGCCCTCCAGTTCTTGCTTAGGAATCAAAGCCGCCACGGAGTCCACCACAATTAAGGCCACTTTGTTGGTCTTAATCAGTGACTCCATTAGGGCAAAGGCGTTTTCCCCATGCTTGGGATGGGCTACTAGTAATTTACCCAAGTCAATCCCAATTGCCTTGGCGTAACTTAAATCTAGTGCACCCTCAGCATCAATGTAACAGGCCACTTTGCCCGCTTTTTGAAAGCTAGCGACTGCATGTAACGCGACCGTGGTTTTACCCGAGGACTCGTTACCATAAAGTTCTACAATGCGCCCTAGTGGCAAACCACCAGTGCCGAGCGCTTCATCTAAATGAAGGCTTCCAGTTGAGACAGTTTTAATTTCACTGTTTTTCTTTGCATCTAAAAAATCAAAGTCTGCCAAGTTATTGTTTTGAATAAAACTGTTGTCTTGGCTAATTTTCTTATTAATCATTTCTTTTTGTACCATTGTCGTTTACATCATCTAAATTGATTCAAAAAGGGAAAAACGCCATTTTTTTAGATAATTAAGGCAAATTTTTATGAAATTAACACTTTGGCTGGTGTTGGGCGCTGTCGGAGTCGGTGCAGTTGGTACGGGTGTGGGCTTTGGTACCAAGTACTTTTTAGACCACAAAGCCAAAAGTAACGTGGACGTTGCGTTTAAACAACGTTCACTACGCAAACAGGTCAATGTGGGCTTTTGGAATGCACTCAACTTCTTGGGTAGTGAAAGAAGTTTCGGCAACCAAAACAGTAAGGTTAAAACCAAGGGGATTGCTGAAATTATTAACCTCCTTAAGTACGACATTGTCGGTTTAGCGGAAATTCAACCATCCACTGAAAGTACTGCTGAACGGTTTGTCGACCAACTCAACGAGTACGCTGATCCTTATACCTCCTGGTCTTATGATCTATCTCCCGTAACTACCAGTAAGACTGCTAGTGAAGGCCAGAAGGAACGGATCTTAATTGTTTACAACAGCCAGAGTGTCAAAATGGAGGGACAAGGTTGGTTTTATGACAATCCCGAAATGGACATTAGTAACTATGTCAGTGATGGGAGTTCTTCCAAAACAAGAACACCTAAGAAAACTAAAACTAGTAAAAAGAAGCCTACTAAAAAGAAAAGTTCAAGAAGCAAATCTTCCAAAGGTAGTAAGAAACAAAAAACTACCAATGAAAGTGAAAGTGAAACTTTAGAACTCAAACTGGAACAACGCCGTGTAACAACACGCTCACAATCAAAGCAACAAAAGGGGCAAGAACAAGCAACTGATCAAACTGATAGTGAGGGTGTGACTACTGAAGAAGGAGCAGATAACACTGACACTGAATTAGTTGAAACCACAGCGGAAACAACCGAACAAGAAGCTACCACGGAAAGTACAAAAGACACTAAGGAAACTAAAGATAGAATCAAAGTGGACTGATCGCGTCCCCCTTACAGTGCTGAATTTAGTACCCAAAAGGGCAAAGTAGTAGTGGCTTTTGGTCATTTTGACTCTCCGGGGGCAAACACTAAGCGGGGTGAAAAAATCGCTAAGATGGGCAAGGGTCAAGGTGCCCATGAATACTTTGAAGCGCAAACTACCTTCAAGGCAATGGATTCCATTAAGCAGCACTTTAACAACGAAAACATCCTGTTTATGGCTGACACTAACATTAAATTCGGTAATCAAGCCGCTGCTTTTGGTGAAAGCAAAGATTACACCTTTTTAACTGAAGATAATGAAGCGTGAAAATCTTCATTGGGTACAAAGAGTGGTTATGCCAACCCTTATGATAAGATTATTACCTCTAACAGCTTTAAGAATAAGGTCTTGAATCAAGCTGAACCCTACTGAAATTACTTTGACCGTTACTTAAAAACTAAGGGTCACAATAGTTATCTGTTTGATCTAGCTAGTTGGGGCACTAAACCTAGAACTATTTCTGACCACGCCCCTGTAGGAGTGTTGTTTTAAGTTAATTAACAGCGCAAAATATAAACATGGCGGATAAATGTGTGACCCTTTTTGGGTTCCGCCATTCATAAGGGCGCTCGGGAAACCGGGAGTTTTTTATTGCTAAAGAATACAAGTAAACGGCTTTTTAGGCAAAAGCTATTTAACTCTCTTTAAAGCCAAGTCCCACTGCTGTTCGTAAAACTGCTTGGCTTGTTTTAACTGCGCCACATTTTGCACGGTACTTTCAGCAGGATCATTTTTCGATCACATTTCAATTAGAATTGGTACTGTTGGTACATACTGGTGAATGGCTTTTAAAGCTGCTTCAAATTGGGCTGTACCTGTGCCAAACGGTACTTCCTTAAACTGCTTGGGTAAAGTATCTTTAAAGTGAAAGCCCACTATCTTGTCTTGACCTAATGCGATTTCATCAAAGACTGCCGTACTAAATTGCGATAGGTTACCAATATCCGGATAGAGCAAAATGTTAGCCTTACCAATCCGCTTAATCAAGTTTAAACAACGCACAATCGTGCCAGCAAACGGCGTGTCCATCACCTCAAAGGCAATGGTAACAGCGTACTTTTGCGCTAACTCTGCCACTTTCCGCATGTTAGTAATGAAATTGCGTTCTGTTTCTGTGTCATGTGGCTCATAATACACATCATAAGCCGCTAACTGGATAATACGAATCCCTAGTTTGCGTGCTAACACACAGGCCTTTTCAAAAATGGTTAAGCCCTGTTGGACAGTAGTTTTATTCTTGGAACCCAAAGGAAAGCGCCGGTGGGCACTTAAACACATTGAGTTTAAACAAAAGGTTTGTGCTTGTAACTCATTGTGGAGTTGGTTAATTTCGGTATCACTCCAATCCAAGCGTTGTAACCTAGCATCCGATTCATCAATGCTCAACTCAATAAAGTCAAAGCTAGCTTGCTTAGCAATAGCAATTTTGTCCTTTCAGGCAAACTTATTGTTAATTGCCTTTTCATAAACACCCAGGAGGTGTTTATTTTCAGTACTTGTGAAACTCATCTTTAAATTGTTGTACGGTTTGAAGTGGATCACTAGCATCCCTAATGGTACGCCCAGCAATAAAGATATAAATGGGAATATCCTTAAATAACTGGATGTCGTTGAGGGTAATGCCGCCGGTAACGGTGACCTTAAAGCCCAAATCCGCTAAGCGTTTCACTGCTTGCAAATCCTTATCTGATCAGTTGACTCCCGCTGCTTGGGCATCACGGGAACGGTGTCACACTACCTGCTGAACTCCCACTTGTTTTCACTGCGTTACCTGCTCCCAAGTGAAGTTACTGGTCATTTCCACCTGGGTTTCTTTTATGGGCGTGTAACTGTTACCCACAGTTACCACATCCTTTACGGTGGGCAATTCCGCTGCACAAATGACCGTGGTAAAGTGTGCCCCAGCATCAAAGAACATTTGGCTAAACACCTTCCCCGCATCAGCAATCTTGCCATCAGCCACTATTAACTTATGGGGATAGCGTTTGGCAATTTCTTTAACAGCATTAACGCCCTCCGCTGTTAGTAAGATAGTGCCCACTTCAATAACATCAACAGCATCCCCGACTTTAGCCAGGTCATTTAAAGCACTCGCTAAACTGAGGTTATCAAGCGCAATTTGGATTAAAGGTAAAGCCATATTAAGTTAAATATTTGTGTAAGTTAACCCGATCAATCACAGCAAACAAGCTGGTCTCATCACAACAGTCCAACAAGTCCTTAACCACTTGAGTATCTTCTACTAGGGCCGCAATTTGTGGTAAAGCTTCTGTCGTATGAATGGCAGCGCTGGTAGCCGCTAATCCAATTAACAAGCGCACCGGGCGGGGATCGTTGTCAAAGTAAACTGGTTCCTTTAGGGTCACTAAAGAGAAGCAGTTTTGCTGTACCGTACCGTTATTCTGGGCATGAGGCATGGCGACATTTTCCGCAATAATGAAATACGGACCGTACTGAGCTACACTCTTTAGAATGTCATCAAAGTAAGCTGGTTGGACTAACTTGGCAGCAATTAAAGGATGGATGCAGAGCTGTATCGCTTGTTTTCAGTCCTGCGCGGTTTGGTGCAACCGCACGGTGTGGTGTTGCTTTAAGACGGCCTTAAAATCGAGCTTTGTCATCTTAAAGCACCTTACTTAACGCTTGCTTAATTTCGTTTTCATCCATCAGGTTTAACACCCCAACAATCTTGGCCTTAGCATTCGGGTTAAACTCACTCGTTAAGTGAATCGAGGAAATAATAATGTCAGCGCTGTGCTCCATCCCCTTGGTTTGTCCCATTGACAAAGCTTCCACTTTGGCGGTGTAGCCCAACTCCTTCATAATCTTTTCAACCTTAATCTTGATTAGCATGCTCGTACCCATGCCGTTACCACAAGCAGCAATAATGTGTAATTTCTTATTTTCCATGGTTAGTTATTTAAATCTAATTGTGGGTTAATTTTGAGTAATTGTTGTAATTTAGTTACTTTAGTTTGCTTACCATTGTCAATAATTTGTGCCAGCAAGATTAAACCAACGGTAGCCAAAATTACTAAGATCCAACCACCAATTACATTGTAAGCCCCAATTCAAACTAACAACCCAAAGTAAAGATTCCAGTCAGCCATTCCAATAAAACCGTTGTCAACGGGTGATGCACTTTTACTGATCCACTCAATTGCTTGTGCTAGCGTACTGTTAACAGCTTTTAAACTGTTAAAGCTATCGGATTTAACAAAGCTTTGCGCTTCGGTTATCCGGTTAACACCAGTAGCATTTACAACCTTTTCTACGGCTGTTTTTAATGCCGAACCGTTGTTATTGTCCAAACTATTACCCATTAACGCCTTTAAATCAAAGCTTTGTTTAGCATCTAACAGTGCCGAAGCCTGGTAAGCGTTTGATACGGTGCGCAGCGCAAATGAAATCACAATAATTTCTAAAAAGCCAATAATACCCGGGAGCAGTAAGGCCGCCTTCCAACCACCCTCGGCATTAGCATACACGCCAAAGGCACCACTGTTAAAGAAGAGTGTAATAAACAAAGGAATCGCCACAAAGCTAAACTGGTTACCCGGAATATAGCTAATGCCAGCCATAATGGCAACAGCTAAAAACTGACCAATGACCCCACTTAAAAACCCAAAGGTCACGGAGTTAATTGAAAACCCATAAACAGCTGCAATATCAACCGCTACCACGGCCCCTGGTACTACCTTTTCACTAATACCTTGAAACGCCTGTTGTAACTCAGTGATAAACATCCTTACCCCAGTCATAATGGCAATTAAGCTGGCCACCATTTTCAAAGCACCCCCAATAATGTTGAGCGTAAAGTTAGCTCCGCCAAAGGTAGTGTTTCAAATCTCTAAACCATTAATGGCACCAAATTTGTCCCAGTTAATTAACAGTGGTTTATCACCCTTGTAACTAGCCAAAATCACAATAAACAACACCACAAACAAACTAAGGATAATGATCGTTTGGGTGAAGATATTATCTTCAAAGATCTTTAGGGCCTTAGGCAGTTTTTTGTGCTCGGCACTATCGTTTTTGTCACCAAAGTACTTGCCCAATTTAGCAACCGTCATAATCCCTAACATCTGCTGGTGACCAATCGAAAAACCAGCATTATTGGTTACCTTATTGGTCACTAAATAGGTACCCGTAGAGGCCGTAGACCAGTAAACTCCCAAAAAGATACCACTAATAATCACTAGACCGGCTTGGGCACCGTAAGCAATGCCCGTACCCAACAACGGAATTTGGCGGAACAAGACAATATAGAACAGCGTAGTAACTACCGCTGCTTGTTGCAACATAACGTGTCCAGTAACCATAATCGAATTGGTGTTAGTTCAACGCTTTAACCCCACAAAGATAATGTTGACAATAAAGCCCACTAACAGCGAAAAGGCAATCAAGGACACATAGTCATTGTTCAAGAAACTGTTCGTAAAGAAATCGTTCGCTGATGCTAAGGAAAAGTAAGGATCCAATAGTACCACACCAGTACCCCCTAAACCCTTAATCGTGTCAAAAACGGGACGGGCGGTGGACACCAACACGCCACTACCAATGCTTAACAGGATAAAGCCAATTACGGTCTTTAGTGCCCCCACCACCGATTGCACCCCACCACGACCTAAAGCAAGGTAACCAATTAGGGTAATAACACCTAACAATAGCGCTGGTTGACGCAAAAAGTTATCGAGGTATACCCGCGTAATTAAAAAGATAAAGGCATCACCAAAGGCTTGGGCCGAAAACGGGGTTACTCCCTTACTAACTAAGTTCGGTAATCCTGCACGCAGTAATACTGTCAAGAGAATTACCACCAGGTTGATCAAAGCAAAAACGGATCAACCGATAATTAACTTACGTTTTTGTTGGAGCTTAATTTTGTTGAGCATTACTCTATTTTTAGTTGTTTTTTAAGTGCTAATACCCGTGGATGCACAGCCTTGGGATTAAACTTCCGCGGTGCATCAAAGGCCAGGATTTCAGCGGGATTTTCGACCAAGATGGTGTTAATGGCCGCATCGGAAACTCCCACTTCTTTAAGGAGCGGAATAAAGCGTTCAAACAGATATGCAAACCCAAAGCACTCCTTCCCTTTTTCTAAACCATAGTGTTTTGGTACAGTACCCGCCCCGCATCTAAGGCCAGGGTAATGTGTTTAACAAAGCCCAAGTCCACTAAGTATTTAATGTGTTTAGCCAATAAACAATCGGGGTAATACTTCACCCGATCGGGCCCATCAAAACACAGGGTCACACCCAGTTCCCGAATAATCTTGGCATAGTAATACTCATCGGGATTCTTGTTTAAGTGCGATAGTTAAATTTTCCGCGGATTAACGCCAAAATCAATCAGGTGTTGTACTGCTTCATATGCCATGGTACCGAGTTGGGTGTGCACGAGTACGGGAGCACCAGTAGTAATCGAAGTAATCGCGACTGCTTCCAAGGCCTTTAATTCCAACCGGTCAATCGCAGCATAGCCCGTACCAGCTTTAATAATGCCTGCCTTAGCCTTGCCCCGTTTAACCACGGGACCGGAATAGTTATACAAATCCATACCCTCTTCTATTTCAGCGACCAACATCGGCACAATTTCATTAACTGGCACTTGTGCTAACCAAGAACTTCCCTTGTCATAGAAAGCAGCCTTGTGAAAACCAGTGGCCATAATAATGTTCAGCTTGCCCTTTAGTTGTTCTGCAATCGCTACCATCCGTTTGACATCACGGCCCACATTCGGTGGATCCATGGTGACAATGCTCCGACCACCATGGTGGACAAATTACAAACACTCTTTAATAGCAGCTTCATTCGATAACATCACAAAATCAGGGTGCTCTTTGGCTTCCGGACCCCAATTCTTAATTAAATGATCGTGACAGTCACAAATACCTAAATCCTTAGGGTCAATGTCCCCTAAAACTGTTCTTACAAATCTTTTCATTTCTAAATGGACGTTAAATGGTTTAAGTGTATTGAAATAACCCTAATAATGTGTAAGCTACTGGATAATTTGCCATTAACAGTTGTAAAGCTGGCAACTATTTTTAGAATCAGCCATGGATCAAAAAATGATAAATGACCTCAAAGAACAGGTTTTTCAAACGAATTTATTACTACCCAAATATGGCTTAGTCATCCACACTTGGGGTAATGTTTCGATGATTGCCCCGAACCGTCAGTTCTTTGTCATTAAACCAAGTGGTGTGAGCTATGACAAGATGCGTGCCCAAGATATGGTAGTGGTCGATTTGGACAACAATGTTCTAGATACCAACGGCCTCAAACCATCAAGTGATACCCCAACCCATGCGTTAATGTACAAACATTGTCCAGATATTAAAGCTATTGTGCACACCCACTCTACTTTAGCGACATCGTTTGCTCAAGCCGATAAGCCGATTCCTTGTCTTGGTACTACCCATGCTGATAACTTCTTTGGACCAATCCCGTGTACTAGGGCTTTAAGTGACAGTGAAATTAATGGAGCTTATGAACATAATACTGGTTTAGTCATTCTGGAACACCTCAAAAACAACCAGGTTGATGTCAATGCTTGTGCGGCCATCTTAGTCAAAGAACACGGTTCGTTTGTTTGGTCCAATAAGAATGGCAAGGATGCAGTGGACCGGGCGTTAACTTTAGAACAAGTGGCCCAAATGGCCTTATATACCCAAATGATCAATCCCCACATGAAAGAAGCAAACCCAGCGTTACAACAAAAGCACTATAACCGCAAACATGGTAAAGATGCTTACTATGGACAAAATACTAAGCAAGAAGATTAAAGTGAACTGGTTGGGCGGGGTGTTTTGGTTATTACCTAACTTACTCGATCTGTTTTCCGCTAGCAAACGTAAAGCGAGTGTGCGACCCTACCAAAGCTTATTGGAACTGGTTCAAGAAAACTTCTTGAACCGGTATGACCTCGTGCACTTTAGCTTTAATGGTGACCATGACTTCTTTCACTTTAATGATTTACAAGCCATTAGGAGCTTTAACTTTACGATAGAAGAGGAACAACTAGGAGCAATGCAACCAGATGAGGTTTTACTGTTTGAACCTGTGGATCGTGTAACAGTGGAATTAGATCAAAAGGGTTTGAGTTTAATCCACAGTGGCAAAGCTTTTTGTGCTAGTGCAAACTACTTTAAGCACTGGCTAAAACGCGTACCACAGCAAGACAAAGTGACCCTAGTGTGGCGAAAATCAGGTTTTGAATTAAAGCAGTAGAATTACTCTAATACTATATAGCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTGCTCGACCAGGTGTTGGACTATATTCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGTGTGGATGATATAACCGCGCCTCAAACCAGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTTTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTCGGGGGCAGTCAGACGACGACTACGGGGAACAATATTCCCAAATGACCCACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGACTACAAGTGGTTCAACCGGCAATGCCAATGACACCAAGTTCACGAGTGCTACGGGCAGTGGGAGTGGGCAGGGCAGTTCTTCAGGTACAAATACTTCCGCGGGGAATCCTGATGGGCTTCAGGCTGATAAAGTTGATCAAAACGGTCAGGTGAAAACAAGCGTTCAAGAAGCCACTTCAGGGGACAACTTGACGAATTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCGCAAGACGTTGGTAACCTCGTTGTCAGTGGCACCAGCTTCAGCTTCCAGCTCGGCGGGTGGTTAGTTACGTTCACGGACTTTATCAAACCCCGCGCTGGTTACCTCGGGCTCCAGTTAACGGGTCTCGATGTAAGTGAAGCGACCCAAAGGGAGTTAATTTGGGCCAAGCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGCTGGGCCGCGTGGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTATACCGACAAGGATTTGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCCCTACCTCCATTTTATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGAGGAGTAGTGGTAACCTCAGTAGTGTGTTTAGTGGTAGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACCAACTTTAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGGCTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTGTTGGCGCTTCTCAATAAACTGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATATATCATTGACAAAGATAACAACAGTGTGGAATTCTCTGGCAAATTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAGTTTAACGAGTACAAAAACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGCGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGCTTGGACAAGATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAGAAAAGAATAGACAAACTTGAGTCCAAATAACCAATGCTCAGAAATAAAAATACCAACAGTGAAACCTAAAGATGCGGCGCTGTTGGTATTTTTTTCAATTCACATAAATAAAAAAGCCATCTGCCCAAGGTGAAAAAAACAGACGGCTTTTTGGCGATTTTGAAATTAACAGCAAGTTGTAACTTTTAGTGATTTGAATGAAAGGAATATATGACCTTTATTCTTTTAAAAAAAGAGATACATCAATTGTGCTGAAAGCCATTGCGTTAACATAAATAAATTAAGACATAAAAAATAAACACATTAATCAAATAAGTGTGGGAACAGAAAATTAGGCTTTCAACACAAAATTTTCGTTTTCCTGCCCCCACTAAATAAATTGAGTTGGGTTTTAAAAAGCGTCAAAAATTTTAATTTAGTTTTTGCTACTTTCCCCTCCCCCTCACGGGTGAAAACCCCGGCGCTTGGGCCTTAGTGCGCGATAACATTGCCAAGGGCATCACTGCCGGTAGTAACACCCAACAAACCACGTATGATCCCACCCGAACCGAGGCCACCTTGACCACCGCCACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGGCTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAGGATAAGGTCCCCGTCGAGGTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAAAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCTATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGAACGCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCACGCAACCGTACCGCCATTGACCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGAGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAAATTAATAAATAGGTCTATAGGTTATATTAGTACACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTAGTGAAGGCGGCCAATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGAGGATACCAAGTTGGTGGCGCTGGTCCGTTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGAAACCAACAAGCCTCCCAAAAAAACCTCACCAACAACCCCGCCCCCAAGGCCGTCACCGGCTTTAAGCTCGACAAGGGCCGCGCGTACCGGAAGTTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACGAAGGATGGCAAGGGGAAGGATAAAGATGGGTGAATCACTTCCGGGGCGAGTGAGGCCAAAGGAGATGCCCCGTTGGTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGGTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCTACAAATGAAGATCTGCCGAATGTAATAACACAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCCCACACCGAGTTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCTTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCAAAGATTGCCTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTTGATGATTGGAAAACTAACGCAAGGTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGTCTGCTCCCACTAAATAAATTGAGACAGATGTTCAAAGTCGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTGTAATCCCTTCTAACTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTTAAGTAATTTAGTTAGTGTAAGCACAAAAACCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCACTACTGCAAGTTGTAGTTTTCCCAACCTCTTTCAGAAAGGAGGTATTGCTTAATGAAAGAGAAAATTTCCGAAAAGGAATACAAAGCTTTAATCCGTAAAACTGGCAAAGAGCACTTTGATGGTGAAAAAGAAGAGTATGGTGATGGCACAGTTGGAGTTTGAACTTATGAATTGCGAAAATATAAACTTAAACCACCTGTGAAAGTTAAATACGTAACCCAAGAACAATTTCAGGAATATAAAGATTCCAACAATCAGCGACTCATAAAGATTGAAAATAAGGTTGATAAATTAGTTGAAATTGTTCAAATTCACGGCGAACAAATTAAAGCACAGGGAGAAACCCTGCAATTAATTTTGCAAACGCTGCAAAAGATGAGTGATCGCTTGGACAAGATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAACTAAAGCCCAGAAATAAAAAGTCATCTTATCAAGGCGAAAGACGGACATCTAATGAATTGAAATCAATTACTTAATTAAATATTCCAAGTAAATAAAGAGATTAATATCTCTTGAGTAGCCGCTGGTATTATTAGCATAGCTACTCAAAAATTGTTTCACACTTTTTTGACTAACATCATCTTTGCCTTTTATTAATCAGCTAAGTGAAGCGCTAAACTCAATTCACTCTAAATTTCTTGTAATTTCTTTTCCATTAACTTTATTTTTTTCTTCTTTAGTAAGAAATTTTTCTGTAGTAAATTGTTTTTTTGGATCAGAATTAGATTTAATTTTAAGGAAAAGATCAAAATAATGCTTGTCACCTTTCTTAGCAGTGAAACCACCTGAAGAATAATCTCCAAACCAATTAAAGTTATAGCTCAACTTAGCTTCAATTGACAAACCCTTAAAACTTTGGTATGTCATTTGAAAATGATCAACCTTTCGCTCAAACTTAAAAGGTTCACTGGTTTTGGGATAAATTACATCTTCAAAATCAAGATCCGGAATACCTAATTCGCCCTTTTCAAAACTCTGTTTTCAGGCGTCTAACAACAATTTTGTTGTATCTTTCGGATTAACTTCGTAAGATTTCTTTAAAGAAGTTATTATGTTCTTTTTTCCTTCTGTAGTTTTAATTGCCTTGTAAACATCAATGTCTTTTTTGGTGGTTTCCTTGATAAGATTTCTGAGATCTGCTTGGATGCCAGTATAGGCGCTTAAAACTGTTCCACATAATAAAAATAGAGAACTAAAGGAAATCCTTGACAACAATCTCTTTTTGCGCATCAACAGTAAAAATAACTCTTTAATTTTGTTTTTTTTTTTTTTGGAAAAGCAACTTTAAAAACGGAAAGCTAATTGAAATTTCGGTTTGAACATTAATTAAAAAACTGAAATTTTCAATCATTTATTAACAACTATCACTGATAAACATCTCGATCTGAGTGGTTCTCATAATTGCCAATTGTTGGTATTTTTATTCATTTTCAAGCTGTATAAAACTTTTTTGCAATTTTGTTGGCACTTCCAAAAGCTCATTTCAATTTATTTAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGTAAAGAATCTGATCCCTGTTGGCAACCGAGTTGCAAGCGTTAGTTACTTTTTAAAAAATCAAAAGAAAGGAGGATTTGACCATTAAAAAAGTAAAGGTTACAGAGAAAGCTTCTGTTGAAAGAATGATAGAAGTATTAAGTGATTCTGAAATTTTGTCTTATTTATTAAGTAATAAGCACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAGCAGCATAAAAATACCAGCAGTTAATGCATAGCTGTTGGTATTTTTACTTAAGTTTTGACCATTTTGAATGGATAAAATTTCCTTGCAAATAGAAATTACAAACCAAAAACTATAACAAAATGAACTAAGCAATTTTCATCTTGGCTTTTTTCTTATTTAAAAGAGATTAAATTTTCATCTTAAAAGCTTTTATTTTTACCACACAGGATCTAATAAATTTCAAGAGTTAAAGAAGTACAAACTTTGTTAATATTTCTCCAAATTTCGGGTTTTTAGTGTTTATGAAATTTAAGTATGGTGCCATCTTTTTTAGTGGCTTTCTAGGTCTTTCTGCCATTTTGGCTGCCTGTGGTACAAAGGGTAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTCCTCTTTAACTTCTAAAAGTGCATCAAAAGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCCTATTGAAATTACCCAAATCGCTGGTGGTTATGATGGCGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAAGACACCACTAACTTTTACAACTTAATCTTAAACTACCCTGATCTAGTTTCAACTTTAGGTCGTGTTGGTATGGAACTGCCGTTTGACAATGTAAAGGTTGACAAACTATCACCCCGTTTTTTAGATTTCAACAACCGCATTAGTGCAATTTCTAAACCAGGAATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAACAACGCTAAAAAGAAAGAAGGTACTTTAAACCAAAAAATGACAAGTTCTTCTGAAGGAAAAAACAGCAGTGGCACTTTAACAGTAGCAACTGATACTGAAACAAGTAGTTTATGGAAAAAGATAGAAGATTCTGCAAAAGCTAACGGTAAAAGCGATGAAAAAGGAAAAGGTAAGAAGAAAGATAATAAAAGTGCAACTTTTTCGCTTGTACAACTAAAACAAACTCAAGAAAAAACAGATGATTCCCAAGACACTAAAAATAGTGATGATCAAGTTAAAAAATCTTGAGGCGAATACCAAGAAGTAGATGGTGTTCTTAAAAATTTTGAATTTAAAGCGAGCATCTTCGAAAATTGACATGACTTACTTGATTTTTCTACTAGAGTAGCTAAATCTTTTAAGAAGATACACGAAAATAGCAACAAAAAAGGTAATGACATTCAGGGCATACTTGGAGTCGACAGTACGCCCAACTCATTGTTTACCTCTGTTTTTGCAGCTGGCGGCGGGGATTACAATAATTTCTTTTACAAGATTGAAAATGGTCGCGCTGACTTTAGTAACTTTAAGAATAAAGGGACTTCTTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTCCAAAAGTTCCACCAATTAGCTTACAGCATTTCCTCTACTTCCGGTTTCTTCTATTCGTTTGCCGGTAAAAGTGCAAAGCGTTTAAATTTTGGTGATAGCTTTATTGAGTATCCGCGGTTTACTCAAGAAATTAAAGCACCATCAAAAAATGGAGAAAATGGCCAAACAAATGAAGGAAATAGTACCAATGGCGAACAAAATCTCTTAGGAACTTTCGAAGTCAAGGATGACAGTAAGCCTAAAGAAGAAGTTAAAAGTAATAAAAATAGTGGTAAAGAAAGTAGCCAAAATCAGGGTAAAAAATCTAACAATAACAAGACTATTTACCTTTATGAAACAAAAATTCCAGATGGAAAAACTGCAGGTGATAATGCTATCTTAATCAAAGATAAGAACGTTATCGAAAAGCTTAAAAGTGCAGCTAAAGAAGAAAATAAAGAACAAACTGCTGAAGCAACTAAAGCTGCAATTACAAGTAATAAAGCAAAAAGCACTAAAAAAGAAAGCAGTAAAGTTATCGGTTACACCACAACTGATAGTGTTCGCGAAGATGGCAAAAACATTTTCGCAATAGATAGAGTTAATGGTGAAAACTATGACCGTAAGATTATTGTAGGTGCTAAAGCAGAAACTTTAAACCAATCTAGCACCTTACAAAGTGAAGAAGCGATTGTTTTGCCTGCCCCTGGAAAGTATCTAAATGGTGATCCCAAGAAAGTGACAATTACCCAAGGACCTAACATCATTGGCATTCATGCTAACGAAAAGGAAAATGCAGAAACGCAAAAGTTCGTAGATTGATTCTTAAACAGTCCGCAAACTTGAGAAAAACAAAGTAGAGATAAGAAAGGATCAAGCGAAAAACAAACTGCTGCTGAGTTCTTTGCGGAATCCGCTTCTTACATTCTGCCCTTAAAAGAAATCTTTGACAAAAACGATACAAAAACAGAAAAAGGTAAGAACAGTAAAACGCAACAGAGAACTAATACCTATGCTGAAAAAGCTTTGGAACTCTTCAAACAAATTAGTCAAAATCAAATAGTAAGCTACAGTGATCCAAGTGACTTTAGGAGTGGAAAATTCCGTGATGCAATTGGTGCTACTTTCAATGCTGCAGTTAGTTCCAAAGCAGACTTCAATAAATTCGTCCAAAACTTTACCGCTACATTAGGTAGTGACATTTAATTCAAAAGCAGTTTTTCAAAATTCTTGATTTACCTTTAAAAGTATCAGCAACACAATAAAATTAACTGTTTGCAAGAGCTCTTTTAAAGCCTTTTAAAAGAAATTAAACTTTTATTGTTGTGCAAATTAGGACTTACAAAATCAAAGATATTTGCGACATTCAACGTGGTCGTGGTATCACTAAGGAATACATTAAAAATAACTCTGGAAAATATCCTGTTTATTCTGCCGCTACTACCAATAACGGTGAGTTAGGTTTTATTAATACTTACGATTTTGCTGGTGAATATGTTACCTGAACCACTAACGGCTATGCTGGCGTTGTTTTCTATCGTAATGGCAAATTTAGTGCATCACAAGACTGTGGTGTTTTAAAAGTAAGAAACAAGGAAATTAACGCTCAATTTTTAGCTTTTGCTTTAAGTTTAAAAACACCTCAATTTGTCCATAATTTGGGATCAAGACCTAAATTAAACCGAAATGTGGTAGCTGAAATCTCCTTAGATTTTCCACCTCTAGAAGTTCAAGAAAAAATTGCTCATTTTCTCAAATCTTTTAATGAATTAAGCAGCCAGTTAAAAGCTGAATTAATTAAACGACAAAAGCAATACGCATTTTACAGTGATTATCTACTCAATCCTAAACATTCACAAGGTGAAGAATACAAACTCTTTAAACTCAAAGATATAGCTAAAAAGATTTTAGTTGGCGGTGAAAAGCCAAGTGATTTCCAAAAAGAAAAGGATCAAGTTTATAAATACCCTATTCTTTCTAATAGTCGCAAAGCAGACGATTTTTTAGGCTACAGCAAAACGTTTAGAATTGCTGAAAAATCAATTACAGTTAGTGCAAGAGGCACAATTGGTGCTGTTTTTTACCGTGATTTTTCCTACTTACCCGCTGTTAGTCTAATTTGCTTTATTCCTAAGCCAGAATTTAATATTAAGTTTTTGTTTCATGCTTTAAAAGCGACTAAATTCCACAAACAAGGTTCTGGCACAGGACAGTTAACCATGGCACAGTTTAAAGAATATCAGGTTTATATTCCATCTTTAAAAAAACAACAAGAAATTGCTGCCACTTTGGATCCACTTTACTATATCTTTGCAAATTCTAATTGAGGAATATACAAAGAAATAGAATTGCGCAAAAAGCAAATGCAATACTATCAAGAACGTTTGTTTCAGTGAATAGAAAACCAAAAAGTTTAATTACTAGTTAATTTTATCCGTTTAGATTTAAGCGCTTAAAAGAACAAAGTGTAAATAGCTGGCTAGATCAACTACCGGTGGTTTGGTAGCTCCGGTGACTAGTTCTAAATCATGCAAGTTTTGTCCTCTCAATAAATTCATAAAGTTTGGATCGGCAAATGAAATCTGTTCTTTGACATACAATGTAAATAACAATTCATCCTTGTCATTAAACTGGAAGAGAGAACCAGGATAGTCTTTTTTAGCAACGGTGTAAACTGGAATTGATCCATCAACAATGAACTTTAAGTTCATTTCACCACTTCAAGAGCTTGGGAATTTAGTAAATAAAGCTGCAAAAGCATAGAAACCATACAATTGGTAACCACCGGTATATTTAATGTCCACTTCAAATCGATAATTAAAGGGATTAGCTTTATCTTGTTTTATTCCAACACCATCTATTCAATTAATGGTTGGACTTTCAATTTTATTTTGAACTTTTAATTCCTTAATCTTTAGATTGATGTTGGATTTGCTAGTAAGTTCACCGCGCAATCAATCGGACATGTAAATTCCATTTAAACCATCTTTAGGGAATTGGATTCCTTGACTACCAAAGGCCTTTTGCTTCGCATCGTTATTTTCTTTTTTAGCGTTGTAATACCAATCAATGGCAGTTCGAAATCCAGCAATTAAGAAACTGAAAGTTCTGTTTCTATCTGCTTCCAAGGAGATTTTTAAAGCCTCTGCTAACTGTTTTTTATCAGTGACATCTTTGTTCTTCTTACTTCAATATTGTTTGAATTCTGATGCAGCTGTTAGAGATTTGTTAAAAGCTTCTCTTTCTGCTTCGAGTTTTTTAACTTCCTCTGCTTTTGCTTTGGCCTCTGCTTCTTGAAGTTTACGCTCCTCTTCTAGTTTCCTTAACTTTTCAACGTGCTCAGCATGTGCTTTTTCTCTTTCTTGCTTGAAAGGATTGAGTACTTTTTCCCTAAATTTAGTTGCAACATCTGTTTGGTTATTTATTTCAATAACTGATTCTGTCAGGTTAACTTTAGTATCAAATAAAGAATCACCTAAAGCAGTTTGAGCAAGTGTAGTTAAATCATTTGTGCCGTTTGAAATTAGCTTATGCACTGATGGTTTTCAAAACAGTTGGGTATTTAAATCTTTACCAGATTGTCAGAAACTGTCTTGTAAATCTGTTAGATTGGCAAAATTAGTAGTGGATGCGTTGAGTGCAAACTGTAAATCTATCGTGCTACCATCACCTTCATAAATAGTTGCAAAATCCATTTGGATGGAAAAACTATAACAAACTTTGCCCTGAATGACTATGTCGTTTTTTCGCTCTAAATTTAAATCAAAGTTTTTGAGTGCAATCGACTTAAGGTTATATTGATCGAATAATGCAGCAATATTGTCAGCAATCTTTAAACCCTTTGGATGTAATTGCGGAAAGAAACCATCACCAAGTAAACCAAAAAGCTTTTGTGACACTTTAGGAATTTTAGTGCTGTCGGAAACAGGAAACACAGCATTAGCTGCATTTATTTCTGCTTGAAGTTGATTCATTAAATTAACCGTGTTTTGCACACTTTGTTTAAAACCACTAAGAAGGTTATAAACATCAATTAAACCTAATGTTTTTGGACTTAAAGCTTGTTTGACAAAGTCCTTTTCAAATTGTTCAAAGTTGTGCTTTCAATCCTTATTTTTCGCTATTACAGCTAACTCTTCCTCACTAAAAGTAGATGCGTTAGCAAGATTGACCAAGTAGCTGTCATTATGGGCTCTAGCTAAACTTTTATTGTGTAGTGCTAAAGTATTAATGTCAGCACTAGAACCTTTTTGAGTTAATGTAGGAAATAAAAATGCGCCTAAAGTTACAGTAGTGGTACAAAAAGCCAGCAAAAACGCTTTTGTTTTGAAGCGAATTTTTTTAGTTGCTTTCAATTTAGTTGAGAAATAAGCTAACTAAATTACTTATTAAACTAACGAAATCAATTAAGAATTAACTTTCAACCACAACAAAGTACTACAACAAACAGGGAAAATGTAATTTGTACGTAGTATTTTAGTTCTTTCTTGTGAAATATTGCAAAAGTTAGCTAAATCGAAATTAGCTCTTATCCGCTAAAATGAGAAAGTGGAGGTAGCTCGCTAAGTCTACTACTGGCACTTTAATGTCACCACCTCAAGGCTCTAATACATCGAGCTGTTGTTTCTTCAATAATCCTTTAAAGCGTTGATCCTGTACATTAATCCACTGAGTAATGTGTAGAGTAAATAACAGCTTGTCATCCCTAAACTGGAATAAGGTTCCAGGATAGTCCTTTTTGGAAGTAATTCAATCTGCTAATTGTCCGTCAACATTGAGCACTAATTCCATTTCACCCTTCCATTCTGGAGGAATTCCACCAAGTCCTCAATTTGCTAAAGTTCAGTAAATACCTTTAAGTCCATAATCACCGGTGTATTTCATTCTGATTTGGAAAGCATATTTAAACGGATAGGTTTGTCTAAACTCAATACCGTTCTTGGCTTCATTTCAGAAAATTTGTTTTGGTACACCCCAACCATTAATGTCATTGTGAATATCTTTTGTAGTTACCGATTGCAGGTTCAACTTTAAGTTAGCTCTGTGTTTTAGCTCTCAGCGTAACCAGTTAGGGATAAAGATTCCATCAACACCTAGTTTTGGTAATTCAATGCCAACATCACCAAAGGCTTTTTTCTTGGAATCTTGATCATCCTTTTTGGCGTTGTAGTACCAATCAACAGCATTTCTAAAAGCATCGGTAAGGAAAATAAAAGTTCTGTTTCTATCAGTCTTAAAGGCCAGTTTAAGTACTTCAATAAGTTGCGATTTAATTTCAACGTTTTTACCTTTTCCTTTTCAGTAATCTTTAAAACTTTTAACGTTATTGAATGAACTAAGAAAGCGTGTTTTTTCCCTAATTAACTCTTGAACTTTCTTTTCTTTACCTTTAAGTTCTTCTTCAAGCTGTTTACGTTCACGTTCTAAACGCGCTTGTTCTTCATCATGAATGCGTTTTGCTTCCTTACGTTGTTCAATGAATGGATTGAGGATGCGTTCCTCAAACGATTTGCGAGCTTTAGTTAAAACCGTTCCATCAATTACTGAATCAGTTAAGTTAACCTTAAGATCAAACAGGTTTTCACCAATCGCTGTTCTTGCAATTGTGGTTAAGTCATTGCCCTCATCAGTAATTAATTTATCTATTACTGGTTTTCAGAACAATTGGGTTGAGAGTGTTTGACCACTATTAGTAAAACTTTCACGTAATTCCTCTAAAGAGCTAAAGTTATTCGTTTGAGCTTGCAGAGCAAAATCAAGGTCAATTTTGGTATTTTTGCCAACATAAACGGTATGAAATTTCATTTCGATGGCAAAACTATAGCGCACCTTGTTCTTTAAAACAATGTCGTTTTGTTTGAGCAACTGCAAGTTAAACGCTTTTAACTTAATTGAATTTAAGTTGTAGTCATTAAACAAAGAAGCAACCGTATCACTAATATTTAAACCACGTGGTGTTAATCTTGGGAAGTAATCTTCACCAAAAAAACTAAAGAAACCTTCTGGTACTTTAGGGATCGTGTTTTTGTCCTCTACGGGAAAAATCTCGTTAGCCGTATCCGCCCGTTTTTGTAAGTTGTTTGCTAAATTTACTAAAGCTTGGGCTTTTTGTTGAAACCCACTAAAGAGGTTATAAACATCAATGAGAGTAAAAGCTTGGGGATCAATAAGTTGGTCAGCAAACTTATTTTGGAACTCGCTAAAGTTTTGTTCTCAATCAGTGCGACTTGTAATCGTTTCGAACTCGGTTTGACTAAAAGACGAGTTGCGCTGCAATAAGTAACTCTTGTTGTGTCGCTTATCAAAGCTACTGCTATTTAAATTAACAGCATGTGGATCATTAGCTGAACCGTTCTGTGATAAGGTGGGTACTAGAAAAACGCTAAAAGTTAGCGTTGCTGAAAATAAAGACAGCAAAAACGCTTGCGTCTTGAAGTGAAAGTTTTTCGAAGATTTCAAAGATTCTTGTCAGTGTGACTAAAAGAAATAAATTAACTTACAGGTTACGAAATGCAGACAAAATAAAAGAAGCAACGGAAAATTTAAATTCAGAAAATTCTTAGTTTTTTTTTTTTAGTTTGAACTCAAAACGGGTTGGTGAAAGAAACTGCAATAAGCTGTTGTAAATAACTTTAACTAAGTTAAAAACTGTTTCAAACTAACCAAGCTTACTTTTAGTGTGCCTTATTGGAAATTAATGTTGGCTTAATTATTTATCTGCCAAAATTAAGAAGTGGAGGTAGCTCGCTAAATCCACAACTGGTACTTTAATTTCACCACCTCAAGGTTCAATCACATCCAATTGCTGTTTCTTTAGCAGGTTCATGAAACGTTGATCCTTAACGGTAATACGTTGCACTACGTGTAGGGTAAACAACAGTTTGTCATTTTGAAATTGGAATAACGAACCAGGGTAATCTTGCTTTTGGGTAATCCAGTTAGCTAGCTTACCATCAATTTGGAACTTCAATTCCATGTCACCACTCCATTCTGGCGGAATGCCACCAAGACCCCAGTTAGCCAAAGTTCAGTAAATCCCTTTTAACCCATAGCTACCTTTGTAATTCATGGTTATTTCAAAACCATAAGTTCAAGGAGTGGTTGCTCTGTAATTAATGCCGTTGTTGTGGTCGTTCCAATTAATCGAAACAGGCACACCTCAACCATAAACCTCAAAGTTGTTTTCCCTTACTTTGACATTTTGTAATTGAAGTTGAATATTAGCTCTGTTGATTAATTCCCAACGTAACCAGTTAGGCATAAAGATGCCGTTAAGACCTGATTGAGCAAAGGAAATGCCGCCGCTACCAAATGCTGTACGTTTTGCTTCTTCATCGTGTTTTTTATCGTTGTAATACCAATTAATAGCATTCCTAAAGGCATTGGTTAACAGTTCGAAAGTAGGGTTCTGCTTGGCTTGGAAGGAAAGCTTTAAGGCTTCGATTAGTTGCTTCTTGTCTGCTACATTTTTACTGCGCTTAGCTCAGAACTCCTTAAAACTCTTAACGTCTCTAGTAGATTGGTAAAAGGCTCAATGTTCTTCACGAATACGACGAGCTTCATCTTCCTTGGCTTTCAATTCAGCTGCAATCCGTTTTCGTTCCGCCTCTAAGCGTCTTGCTTCTTCATCATGAATGCGCTTAGCTTCAACTCGTTCAGCGTGGAACGGATCTAAGATCTTGTCCTCAAATGATTTTTGCGCTTGCTGTAAAACGCTTTGATCAATTATTGAATCAGTTAAGTTAACCTTGAGGTTAAACAAACTTTCACCAATTGCTGTTTTAGCAATGTTGGTAAGATCATTGCCACCATCTGTGGTTAATTGTTCTACCACTGGCTTTCAGAATAGATGGGTTGAAAGAGTGTTGCCATTGTTTTCAAAACTATCACGTAGTTCTTGTAAGCTAGTGAAGTTTATTGTTTGGGCTTGTAAACTAAACTTTAAAATAGTGGTGTTCTTTCCAGCGTAAACCGTGTCAAAGCCCATTTGAAGAGCGAAGTTGTAACGCACCTTATTCTTTAAAACAATGTCGTTTTGGCGCAACAGTTTTAGGTTAAAACCAGTAAAGTCAATTGAATTTAAGTTGTAGTCATTAAACAAGGAAGCCACATTATCGGCAATGTTCAAACCACGGGGAGTTAACCGCGGAAAGTAATCTTCCTGAAAGAAACTAAAGAAGTTTTCCGGCACTTTAGGGACAGTAAGCATTGTCCTCTACGGGAAAGATTTCGTTTGCCGCATTAACTCGTTTTTGCAAGTCATTGGCTACATTAACTAAACTGCGTGCTGTTTGTTCAAAACCACTAAAGAGGTTGTAAACATCTGTAAGTGTAAAATGCTCTGGGTCAATTGCTCGTTGGGCAAACCGTTTTTGGAATTCACCAAAGTTTTGCGCTCAGTTAGTTTGCTTGGAAAGGTTTTCATACTCAGCGTTACTTAAAGCAGTTTTGCGCTTCAATAAGAATTTCTTACTGTTATGCTTTTCAAAGCTACTGCTGTTTAAACTTACAGTTTGTGGATCAGTAGCTGAGCCATTTTGGGAAAAAGTGGGTACCACAAAAACACTAAAAGTTAGTGTTGCTGAAAGGAGCGAAAGCAAAAATGATTGCGTCTTGAAGTGGAAACTTTTAGTTGGTTTCAATGTAAAGGTGTCAGTGTGACAGAAAAAGATTGGTTTACTTTTAAAAAGTGAAAACTAAGACGACAGGGAAAATTTAAATTTAATAAATTTTAGTTTTTTCACCGAACAAAAAACGGTTTCATCGGAAAATGAAGCATAAACGGCTCAAACAACTTTAAGAATGTTTAATGATGCCTTGATTACTGTTGATTTAACTTCAACAGTTGGTAGTGTAAATAACTCGCTAAATCCACTACTGGCGGCTTTGTGGACTCGGTACGTAGTTCTAAGTTATGGAGGTTTTGTGACTTAATTAATTCCATGAATTCTGGCTCTTGCACCGTAATTTTTTCTCACACATGGAGCTTAAATAACAGCTGTTGCTTTTGATCATCAAACTGGAACAACGAACCTGGGTAATCGGGCTTTTGGACAATCTCTCAACTTAAATCACCATCAACAATAAATTTTAAATTCAATTTACCCTTTCACTCTCCGGGAATACCAGAAAACTTGGCAATAATGGCTTCTAATCAACTTAATTTGTAATTACCTTGATATTCAATGTCAATCTCAAAGCGGAAGTTGTAAGGTTTGTCTTGATTTCGCTTAATACCGATATCTTTGTTCCATTTAATGGTAGGACTTATCTTTGAATCAGTTACCTTTAAGCTCTTTATGTCTAAGTTAATCTTGGCCTTTTCCCCTAGCTGTTTACGCAACCATTCCGACATAAAGATGCCCTTTAAACCAGATTGGGGAAAGGCGATACCTTGTGTACCAAAAGCAGTTTTAGCAGCCTCACTTTGATCTTCTTGGTCATTTGGGTAATAATTGCTAATACCGTCCTGGAAGTTCTTAATCAAAAAGGCAAAGGTCTTATTTTGTTCTTTGGCAAAGGAAAGTTTTAAAGCTTCTAACAGCTGCTTTTTATCGGTAACATCTTTCTTTTGCTTTTGCCAGTATTCTTTAAAGGCTTGCACTGACTCAAGCGATTTGCTTACTTCTGCTAGCTATTCAGCTTCCTTTTGTTTTTGTTTTAATTCTTCGAGCTGTTTTTGGCGTTGCTCCTCTAAGACTTTTTGGTGTGCTTGGTGTTCCGCTAGTGCCTTTTGCCGTGCTTCAATGAACGGTTTTAAAACAGTTGCTTCAAACCTTGATTCAGCCTCTTTTAGCACGGTTTGATCCAAAACTGACTTGGTTAAATTAGCTTGGGTGGTAAATAAATTATCACCAACAGCTGCTTTTGCTACAGTTGTTAAATCATTGTTATTACCCAACACTAACCGCTTGACCGTTGGCTTTCAAAAAAGTTGTGTAGTTAAGTTCTGGCCACGTAAAACCAAGCTGTCTTGCAATTCCTGTAAGTTAGCAAAATTAGTGGTCATTGCCTTAACTGAAAAAGCTAAGTTGGTTGTACTGTTTACACCCGTATAAACTGTTTCAAAATCTCATTGGATTACGAAGCTGTAACGCATCTTGTAGCGCTTCACAATGTCCGGTTGTTTTTCTAGTTTCAGATCAAACTGCTTTAACGCAATGCTTTGAAGTTTGTATTGTTGCAAAACTGTCGTAATATTCTCAGTCACATTTAAACCCTTAGGGGTTAATTGAGGAAAGAAATCACTACCCAAAATGGTAAACAGCTGTTCGGGTACTTGGGGCAATTGAAATGCCTCGTGCACCGGAAAAACGGCATTTGCTAGTAATACCTTTGCATTTAAGTCATTAATTAACTTAACGGTATCACGCGTACTTTGTTCCAATCCACTCAACAGGTTGTAAACATCAATCACACCCAACTGTTGTGGGTTAAGCGAATTGTCCTTAAACTTAGTTTGCAACTGTTCAAAGTTAGCCTTTCAGTTAGTGTGTTTGGTGGTGTAATCCAGGTCAGCTTGACTAATACCAGAATTAAATTGCAACTGGTAGCGGTTGCTTTGGTTTTGTTCAAAGCGTTGGTTGCTTAAGGTAAAGTCACTAACTGACTGGTTTGTTTTTTCAGTTAAAGTCGGTCAAACAACGATAGCTAGAGTAGTTGTTAAACCAGCTAAGCTTAACGATAACGTTTTGAGAAGTAAACGTTTCTTTTTAATTAATTTCAAGTTTGGTTAAAAACGGTTTAACCAAATAGTTAAATTACTAACCGAAATGAATTAAAGCAACTACAACAACAGAAAAATTTAATGCGTTAATAATTGTAGTTTTTGTACTTTACTGTGTTGACAATCAACTGTTAAAAACAAATAATACTGTTAGCTTTTATCGATTTGTTGCACCAGCCAGTCTTTGATTTCGCCATAATAACTAGAGATACTGCGCACTAGTGCTTGACCCACTTTTAAACTTTTAATTTCACTAGCACAGGTAATATCAACCCCATACAGGTTAGCCACTTCTTCTGCTTCCATCATGTCAATTAAGCGGTGGGCAATTAGGGTACTGGTGTTGTTAATAATCTGTGATTTAAAGTTATCACCACCAATATTGCGCAGGTTAGCAAATGATTGATAGGACAGGATAACTACAGTATTGAACGAGCGGACTTGCTCCAATACCACCCCCACATAGTGGCTCCCAAAATTACCAAATTCATCAGCAATAAAAAAGGTCGAGTTATCACTCGTTTCTTCCAAACTCGTAATTGTTTGGCCAAAATCCAAAAAGATTAACTTGCCCAAGTACTTGGCATTAATCATGTCGTACATGCTGCTAATGGAAAACAAAACAGCATCTTGGCAGTTTGATAATATCTATCAATCCCACACCATTAGAATCGATTGAACCAATTTCCGCTAGTTCCCTGAGGTTTTCTCTTAAATTTTCAAAGTCCTTTGTGTCAATGGTTTGAACATGCTCCGTATAGTATTCATCAATTTTTTCTACAAAAATTTGATTGGAAGGTTGAAAGCGACCTTTTTCTTTCATTTCTAAAATTTCTTTTCGGCGTTTTTCTTTGTGAATTTTTTTAAACAAACTGGGTTCAAATTTAGCAAAAATATCAAAGTCACGTAGTAATACTTCATTTGTCCTTTGCTACAGTAACGCGCTAAATTCTTAAAACTCAAATTGTGGGGACCTTCCTTTGCAATTACTAAAGGTACTAAAACTTGCAAAATGGCCTTCTTATTACGTCAATAATAAGCACTGTCCTTGGTGTTTTCCACCATATCGTTATAACCAAACATTTCCAAGATTAAAGTAATGATTTGTTTGGGTGTTTTTTACTTAAACGGGTTGTACTTAATCGTGGCAATTCTGTCATCTAAGCTCCAAATTTTGGCACTAGTATTTTTGAGTGCCACTAACTTAACCGTATCAAAACCGTCTTCGGGACTTAGTTGTTTGGCGCTCGTTTGGTAGTGTTCAAACTGGGTAATTAATTCCTTATCACCCTTACCATTCACAAAAATTAAGGGATGGTCGTTGTCAATTGCATACTTCAAAAAGTGCAGCAAGATATTGGACTTACCAAAACCACTCACACCTAGTACCGAGACGTGCTGGGGGAGCTTTTCCAGTTCCAAAAAACACTCCTCGTTTAAAAAGATTTTCTTACCAGTCTGGTTATTGATGTCCTGTTCCATTACTAAAAAATAATTTGTACTGCAACTTCAAAGTTAACTTATTCTAATTTTGCTAGATCATTGATTTCATTCACAGTTTCCCAACTTAGTTTTCACTGTTCAAACAATTAAAAAAGCACCAGCTAGTGCCAGTGCTTCTTTAATCGATAGTTTAGTGGTTTTTAATACTCCTCTTGAACCGTTTTGGCACCGAGGTAGGCCAATTCTTCATCGGTTAAAAACCCAGTACCAGCCGGGATCTTACCCCCAATGATCACGTTTTCTTTCAACCCTAAGAGGTAGTCAACTTGGGTTTTTACCGCAGCATCGGTCAAGATCTTCTTGGTGTCTTGGAAGGATGCGGCACTCAAGAAGGAGTTCGACTTGCTGGCCGCCTCATCGATCCCAAACACCTGGTTAATGGCAATCACTGGCATCTTCCCCGACAGCAACAAGCTCTTGTTGAGTTCATTGAGGTGGTGACTGTGTACCAGTTGACCCACAAACAGGTTACTGCTGCCAGCATCGGTTACTTGGAGTAAGCTCGTCAACTGGCGGATAATAATTTCGACATACTTATCAGCAATCTCAATCCCCTGAATACGGTACACCTTTTGGATTTCGACAATCATGTACTGGCGTACCCGTTGGATCCCAGCAACCCGTAATAACTGGCGAATGTCAATCGAACCTTCGGTAATCTTCGAACCTAGTTCTACTGCATCACCGACCTTGACACGCAGTTGCGCACTAAACGGAATCGAGTAAGTGCGTTCATCGACACTAGATTCAATCAACACTTCCTGCGCGTTTTGGGTGGTAGTAATCGATTTAACTACCCCCTTAACTTCAGAAATGACACAGCGCTCGTAATCCTTAGGGGCGACTACTTCAAAGATTTGCTTTAAACGTTCAAACCCTTGGGCCAAGTTGTTTTCGGTCGAAACCCCCCCGGTGTGGAAGGTACGCATCGTTAACTGGGTTCCTGGTTCACCGATCGATTGCGCTGCAATTACACCCACAGCGGTACCCAATTCCACCAGTTTGCCGGTGGACAAATCGACCCCGAAACAGTACTGACACACGCCGTTTTCGCGTTCACAGTAAATCACAGAGCGCACTAACACTTGTTTAATCGAAGTGGCACAAATTTGCTTGGCTAACTCTTGGGTAATTAGGCTGTTAGCAGGGACAATTTCCGCCTTGGTTTCGGGATCTAAAATTGGTCCAATCGTGTAACGGTTGACAATGCGGTCAAACAGTGATTCCACCAACGAACGGGTTTTGGTTTCCACAATTGCTTCGACCACAATTCCCTTGCGTGTACCACATTCATCGTGGTTAATAATCAATTCGTGGGTTGCATCCACCAGTTTACGGGTCATGTAACCAGACTTAGCCGTTTTCATGGCGGTATCGGTCATCCCCTTACGCGCCCCATAAGATGAGTTGAAGTACTCGTTAATCGTTAAACCTTCCAGGAAGGAGTGCTTAATAGGAACCTCAATCGTGTCCTTAATAATTTTGGACTGGTTGTTGCGTTCATAGTTAAAGCTCTTACTCATTAACCCCCGCATCCCAAACAGCTGGGTAAAGTTGGAGATATTACCCCGCGCCCCGGAATCGGCCATGACAACAATCGAGTTATCACGGTACTCTTCACGTTTAATTAAGTCCTGGATCTCACTCGACACCTTTTCCTTCACACCGTTTCACAGTTTCACCACCCGTTTATACCGTTCATCATCGGTCAGTAAACCCTTGTTGTAAAACTGCTTGTACTTCAAGACCTGTTGGTCCGCTTCATCAAAGTACTGCTGTTTGTTGGAGTACTTCGGAATATCAAACACCGATACAGTGGTGGAAGAGTGGGTCGAGTACTTAAAGCCTAAGGCCTTAATGTTGTCCAAGATCCGTGGTAGATCCTTAAATTCAACCACCGTGTAAAGTAAGTCAATTAACTTGGAGATGACCTTCTTACCAAAGGCAGGGAGCACTTGGTGTGCGGCAATCGCAGCACGCACATCACCACTTGGTGGTACTATGAACTTTTGCGGACACGCATGTTCATCAAATTCCCCTTCATTAATGTAAGGGATGTTGTCCCCTAACACATCGTTAAAGATAATCTTACCAACCGTAGTGATTAGAGTACCTTGAGCTTCAAAGTGCTTGTTTGGAAAGGCTTTGGTGCTAATGCCGACAATGGCGTGGAGGTGCACCTTGCCCGCTTCGTATGCCGCGCGGGCTTCATGCACGGTACCAAAGATGATGCCTTCACCCGTTTGACCCTTACGCTCAGTAGTGAGGTAATAGTTACCTAACACCATGTCCTGGGTTGGGGTGACAATTGGCCGGCCATCTTTTAGTCCCAAGATGTGCTTGGAAGCTAACAGAATAGCACGCGCTTCGTTAACGGCATTTTCGCTCAACGGAACGTGCACCGCCATCTGGTCACCGTCAAAGTCAGCGTTAAAAGCAGTGGTCACTAACGGGTGTAGGCGAATCGCTTTCCCATCGACAATCCGCGGTTCAAAGGCCTGTATTCCCAAACGGTGCAAAGTAGGTGCCCGGTTTAATAACACCGGACGGTCCTTAATGACATCATACACAATCCCCCAGATCAGGTCGTCCTGATTTTTAATCATGTCCTCCGCTTGCCGAATCGAGGACGCAATTGGTCTAATTTCGTTACCGTTGCTGTCAAACTTATTAATTAACCCGTGAATAATAAATGGTTTGAACAGCTTGAGGATCATTAATGCTGGAATCCCGACCTCATACATCTTTAGTTCTGGACCCACCACAATTACACTCCGACCAGAATAGTCCACCCGTTTACCTAAAAGGTTGTGACGGAACAACCCTTGTTTCCCTTTTAAGCGGTCGGTGAGTGACTTCAAGGAACGTTTGTCTTTGGATAACGCTGGCTTGTGGCGCGACGAGTTGTCAAACAAGGCATCAACTGATTCTTGTAACAAGCGCTTTTCGTTGTTAACCACAATGGCAGGTACGGTACCATCTTCCAAGATGCGGCGTAAACGGTCGTTACGGATAATTACCCTTCTGTAGAAGTTATTAATGTCACTGGTGGTAAATTTAGCACCATCTAACTGGATAATCGGCCGGATGTCTGGCGGGATCACTGGCACCGTGTGCAGGATCATGTTTTTCGGGTGCAGCTTCGAATTTCTAAACCAGCTAATTGTTTCCAATTGACGCAAGATCTTTTTAACCTTAGCATACTCAACACTATCCTTCTTAGCCTTCCGTAACGCATCATTGAGCTTACTAAACTCATAGTTAAGGTCAATCTTGTTGAGCAATTCTAAGATGGCTTCAGCCCCGATACCAACGCGGAAGCCAGTGTACTTCTTAATGTAGTTAAAGGCATCATTTAAGGAGAACGGTAACGAACTGTTCTTTAAGCTTTCATAAAAGATCTTCCCCTTGCGGTAGTCTTCGCTACTCCTGTTCTTAATCAGGTTCCGGAAGATGTAACCGATCACCCGGCGCATTTTTTGCCGCGTGGTTAAAGAACCCTTGCCTGCTAAGTCCAACACTTCCTTAAACTTAAACGGCATGATCTTGGGATCTTTGATCTTCCCAGTATCTAATACTATGTAGTTAACAAAGTACAAGACCTGTTCCACTTCCTTATAAGAGATGTTTAACACTAAGGAGATCTTGGAAGGGGAAGGCAGTTCCTTCGACATCCAGATGTGCGCTACCGGACTGACTAGGGCAATGTGGCCCATACGTTCCCGGCGCACAATTGATTCGGTTACCCAAACACCACAGCGATCACAGCGCACGCCACGGTACTTAATCTTCTTAAACTTCCCACAAGCACACTCATAGTCCTTCACTGGACCAAAAATGGCTTCATCAAACAAACCACCAGGTTCGGGTTTTAAGGACTTATAGTTAATCGTTTCCGCCTTGGTAACTTCCCCTTCGGATCAGTTTAGGATCGTGTCGTTGGAAGCAATCGAAAGCTTAATTGCCTTAATGTTCTTGTACAGCTTGTTGTTCTTTTTATTACGCTTTGTCATTAATTAATAACCCTCCGTGTCAAATTCAAAGTCATTAAAGAGATCGTCCTGTTCTCCATCAGCTTGGAGAATTGAAACGTTGTTGGAATCTTGTTGGGTGTTGTCATCGTAAATAAAGGACACACTCAACGCTAACCCTTGTAGTTCCTTCGTTAACAGTTTAAACGATTCGGGGATACCTGGTTCGGGGAAGGCAGCGCCCTTAACGATCGCGGCATAGGCCTTATTCCGGCCCTGCACATCATCGGACTTAATGGTAAGTAATTCCTGAAGGTTGTAAGCAGCGCCGTAAGCTTCCAAGGCTCACACTTCCATTTCCCCAAAGCGTTGGCCCCCATTTTGTGATTTACCACCCAATGGTTGTTGGGTAATCTTGGAGTAAGGACCGACAGCGCGAGCATGGATCTTGTCATCAACCATGTGGTTTAACTTCATCATGTACATGATTCCCAAGGAAATTGGTTTTTCAAATGGTAAACCAGTGCGACCATCAATCAGCTTGAACTTCCCGTGCGTTTCCGCTGGTTTCAATCCTGCTTCATCCATGATGTCTTGCAAGTCTTGGAAGTTCACCCCTTGGAAGATCGGGGTCGCTACTTTAAAGTGGAGGTCATCAAAGCTCATGCCGATTTCCTTGAGCACTAAAGCAATGTCACCGTTATTGAGTTGGGCCAAGGCTGCCTGCTCGTCTTTAAGACCGCGGTTCGTAATTTGGGCTTTTAAACCAGTAATTAAGCGGTCAAGTTTGTACTGTGGCTGGTTAATTTCGGTTGAAAGTGCTTGAGCCTTCTTGTCATCAAAACAAGCACTAATTAACATTCGCTTGGCAAGGTTATGTGCTGCATAACCCAAGTGCATTTCAAAGATTTGGCCAATATTCATCCGACTCGGTACCCCCAGTGGGTTTAAGAGGATGTCGACTGGAGTACCATCTTCTAAGTGGGGCATGTCCGCTACTGGGACAACCTTAGAGATAACCCCTTTGTTACCATGGCGCCCTGCCAACTTATCTCCGATTTGGATCTTGCGTTTTTGCACCACATAGACCTTAATCATTTCGATGACACCATCATTAAGTTCATTACCGTTGGCAATGCTAAAACGCTTGACACAGGACACAATCCCATCACCACCATGAGGGAGCTTTAAGGAAGAGTCACGGACGTTTTGCACACTTTCAGGGAAAATGGCCTTAAACAGCTTTTCTTCAGGTGATACTTCAACCTGTCCCTTAGGGGATACCTTACCGACAAGGACATCACCTTCCTTAACTTCAGCACCGACCATGATAATGCCGTTTTCATCTAAGTAACGCTTGTTAGCATCACTAACGTTGGGGATATCACGGGTAATTTGTTCATCACCGTTTTTGGTCGATAAACACTGGGCTACGTATTCGTTAATCGTTAAGGAGGTTAGAACATCATCCTTCACTAAGCGCTCGGAAATCACTATCGCATCCTCATAGTTGTACCCGTTTCAGGTCGTAAAGGCGACAAGGACATTTTGCCCTAAAGACAGCTCACTCTTGTTAACTGCAGGACCATCAGCAATCACTTCGTCCTTAGTTACCCGTTGCCCCTTTTCTACTAAGGGAACGTGGTTGTAGCAGGTATTTTGGTTGGAACGTTCAAACTTAATTAGATCCAAAGTGTGCTGTTCACCACTGTCACTTTCAATCGTGATCTTACTGTTGTCTACATACTTTACCGTACCTGAGCAAGGTGCTAACATCGTCAAACCGGAGTCGCGAGCAATCTTGTACTCTTGACCTGTACCCACAACGGGAGCGTAAGGCTTAATTAAAGGATAGGCTTGGCGTTGCATGTTGGTACCCATCAATGCCCGCGCTGAGTCATCGTTTTCCAAGAAGGGAATTAAGGACGAACCGATGGACACTACCTGATGAGGGGCCACGTCGATGTAGTCAATCTTCGAGGGATCATACAACCCTTGCATCGAGCGGTAACGACCAATAATTTCCTTATCTAAGATCTTGTTGTTTTCGTCAATGTTGACCAACGAAGAGATTTCGGCAATAATGTGCTCGTCTTCCCTTAATGCCGTGAGGTATTCAACTTCATCGGTAATGACACCATTTTTAATCTTGCGGTAAGGCGCCATTAGGAATCCGTTTTCGTCAATCTTGGCAAAGGAAGCCAACGACATGATTAAACCAATGTTCATCCCTTCCGGTGTTTCAATTGGACAAATCCGACCATATTGGGAATAGTGCACATCCCGGATATCCAAGTTCGGGTCTTCGCGGGAGATACCCCCTGGACCCATGGCGGAAATCCGGCGCTTATTACTTAATTCTGAAAGTGGATTTTGGTGATCTAAGAACTGGGTCAACTGGTGAGTGTTGAAGAAGTCACGAATGACAATTTGAATAGGTTTGGAATTAATTAAGGATTTAATGGTCAATTCCTTCTTTTCCGCTTGTTCAATTACTTGACCCTCTTCGTTAATTTGTTGACCACGGTTCACTCCATCAGCAATCGTGAGCTTTTCCTTTAAGAAGCGCTCCATGCGGGTGATTCCACTTTCCAAACGGGAACTAATTAACTCGTTAATTAACTTGACGCGTTTGTTACCTAAGTGATCAATGTCATCGTACTTACCGATGGCCGAAGGGATGTTAATCACATAACTAATCGAGGCAATAAAGTCACTTAAAGTAATTGCCTTATTGAGATCGTTATCGTTGTGGATCCCCACCACTGGCACGGATACATCGAAGTTGTCGTTGTTCACATAAACATCAAGTGATTCATACAACAACGATTCAGGGGAAAGGTTAACAGCACTACCATCAGTGGTTAACTTAATCTTTTGGACAAAGTCAATTTGGTTGTTTTGGGCAGCTTGCTTAATCTTGTCGATTTCTTCTTTAACTAATAAGGTGCCCTTCTTTAATAAGAGTTCCCCGTTTTTTAAATGAAGGTCACAAGCAAGTGTTTTTTGGTAAATCCGCTCGGTAATTCTTAACTTACGTGATACCTTATAACGACCAGCAGCGGAGAGGTTATACCTTCTTTGACGGAAGAAACGTGAACACAGTAAAGCGTGGTAACTGTTTTCCGGACATTCATCGGTGTTTTCTAAACCACGTAGTGAAATGGACAATTCATGAACAATGTGTTTAGCAGCCCGTTCGGTGATGATTTTATCCATCAACTCATCCATGTCCGCTTCTAACTGACCTACTTTAGGGTCATTTGGTTTAGCTTCACTGAGCGCTTTGTGTTCCTTTTCTAAAAGGACATAGTCACTCACTAAGCTGCGGATCTTTTGGTCAGAGGCAATCCCCTTACGGCGGAGATCAGTTTTGCCATCGCGGAATTCCCTTAATAAGTTTTTAATTTCAGGGTCAACGTTGTTTAAGAAATCCTTAGCGTTGTAGACTTCCGCTTCTAAGGAACGGGTAATAAACTCGTTGTTTTTGAAAACTTTGAGGATTTCCTTGCCACTCATCCCAAAGGCTTTGAGCAAGGTGGTAATCGGGAAGATCTTTGCCCCTTCACGTACAGCATCCCGGAGCAAGATTTGCACAAAGGCATCTTCAATCTTCTTTTTATTGGAGATGTAGATCAGCATTACCGAACCGTTGGCTGGTAATACTTCACATACATAACCTTCTTGTACCCGTTTACGCGAACTAGACAATTTTAACTGGGACTTAGTGAGCATGTAGATCCCCGGTGAACGGGTAATTTGGGCAATCACAAACTTCTCAATCCCGTTGACAATAAAGACCCCATCGCGGGTAATCAATGGTAAGTTAGTTAAGAAAACGCCATCGACACTACTGGTGGCACTGTTCTTCTTGGATTTGCGCGGCTTTTTAATGGTTCCGGTAGCGGAATCAATTAATTCTAAGTCAGCGTAAATACCAATTTCATAGGTCTTGGACTCAGAGCGTGATTGGGCTTCATTACGTTCAGGAGCGGTACGACGCAAACCCTTAAAGTTGATCGTGTAACGATCATTAGGGGACTTAATTGGAAAATAAGCAGCAATCAGGTTTTCCAGATCGTGATCTAGAAAAGTTTGATAGCTCCTAATCTGAATATCAGCCAGATTAGGTTGCACAAAATCAGTGGCAATTTTGCCATAGTAACGGCGGGTAGCAGTGGGTGAGTATTTTTTCTGAAAGAAACTAGGTTTTTGTGACATTCGTGTACAAAAAAGGGCTAAAAATAACAGTAAAAAGCCACTAACAGCGACAAAAGGGCCGCATGCTAATGGGCTTTGTAAAGTTTGCTACATTATATCAATTTTTGTACGTCAGTGATAAATGGCTGAAAATCAAAGCCATCATGATAGCCATTTACTAACACGGCTCCCTTAACGTTGAGACCACTAAAACTAAAGAATTTAGTGAGTAGTTCCAGCACTGTCTTACCACCACTCTTAGCTGCAGGAGTGACACAGCAAATAATTCCATCAAGCTCCTTTAAAAACTGGTTTTTACCGTATTGGGTATCGCGGGTCATCCAGTCGATTGTGTTTTTAGCAAAGGCAGGGACAAAACCGTTGTGTTCTGGAGTGACAAAAATTAGCGTTTTGTGATCGTGTAGTTTGCGCACTAAACTCTTAATTTTTTCGGGAAAATGATCCTTTTCCAAATCTTCACAATAAAAATCCACTTGGTAGTCAACTAGTTCAATTAATTCAGCATTGAGTTGTTGTTCCAAAGCTTTAGCAAACTTCCGGTTAATTGAATTTTTGGAGTTAGCTAACAATAAGATTAACGGTTTGGTACTCATTTTTTTATCTTTGATCCTATTTATATAAAAGAAAATTGTTAATTAAAGCGGAGAAAACTTAATTTAATTTTTAGTTTTCTTTTTTGCAACCAATTTCGCTTTAGCAACCCACTCCTCATTACTTTTCCTTAATTCTTGAGCTTTTCTTTCTTTGATCAACTTAATAAGTTCATTGATTTTTGCTTGTGCTTTTTCCTTATAAAACTCATCAGTAGCTCTTCTAACAGCTTGCAGTTATTCTCTTTTATCGTGATCAAAGGTGGTATATCATTCTGAGTCCAACTCTAATAAAGCGTCTTTTTTAGCAGAAGCTAGTCGTTCTTTAGCTTCTTTGGCATTTCAATGATCTTCTGATGGTAGTTTGGTGTCCATTGAAGATTTAAGGTACTTTTTTAATTCTTTGAGATGCTTCTTATCTTCGTTCTCTGATCATTTTTTAATGCTTTTTAAATGCTTAATTAAATCTTTAGACTGATCGTCTTCTTTAAAGCTTCCTCCCTTTCTTAGACTTTTGAATAAACCTTCTATTCTATCCAATACCTCTTCTTTATGATCATCCTCTAAGTTTTCCTCTAAATTTTTCTTAAATAAGAATTGAAAACAATAAACATCTTTTTTTATTAGTTTCACAACTACCAAAACTTTCACAGCTAAATTTATCAGTGTCTCACTTTTTTAATTCTTCTTTCGTTTTTAATTCAATTTCTTGAATTCTTTTTTTTGTTTTGCTTGTTCTTCTTTAGCTGCACACCCTCGGAAAAACTTATTAATCTCATCTCTCTTTGAGTTTTCCATTTTTTTCAATATAAAACAAAAGAGCACTCCACTAATGTGAAACGCTCAATTGTTAGAGGCAAATACTTATACCTCTAAATCTTTTGAACAGATTTCTATTAATCTATATGCTTGAGAAAGATATGGATAGGGTCTCAAGAAAAGAACATCTGAATAATAAAGCTTTGAAGTTTTAAACTTCTTAAACCCTATATTCATTTTTTTCAGAAACTCTTTCCAAATTCTTTTCTTAACCACTTTATTTTTAGTGCTTCAGATATCACAAATAAAGCTAATACGCTTTCAAAGTTAATGCTTTTAGTTTACTTTTCAATAAAGAAACAAGACTCGTATTGCTTGATGTCACCTATTAGAGTATTAGTGCTTACGGTGGTTAAACCAACGTTTTGATCTTTATTGGCCTCTAATAAACAGATGCAATTATTGTCATAGTTGTAACCCTTCATTACTTCAACAATAAAATAGGCATCTTGTTTAATCACAATTGCATTAACTTGTAAAGTGCTAGCTTCTTGGGCAAACACTTGCAAGTTTTCAAAATACAGCAATAATTGTCCCGCAAAGTTAATAAAGGCTTGCTTTCAAATGGTATTGTTATCTATTTGTAAGTGGTGCTTAATACTCTCCAAGTTTTTGAGTAAATACTTGTGTGGTACGTTGAAGTGCACATCAATGTCTAAGTTATTTTCAGTATTAAAGCGTTCTTTGAAACTGGTAAAGCTCAGGTCAAAACTGTTACTGTTAGCCATAGAAGTGCCAAAAGCAAACTGTGAATTTAACTCGACAAAGAAAAGGTTATTCTTTGTTTTTTGCTTGCGCTTTTGGTGGTTCCAAGTATTAAAAACTTCTTTTCAAAGTTTTTGCGGCAATAACGTTTGTACTGATAACCGTGCTTTTTAATGATTTTAACGATTTCATAAGAAAGCTTTGTTCGCTTATTTTCAAACGGAGAATTAACTAGTAAAACCACTAATTTCAGCCCGGAAAACTGTTTAACTGCTTCTAACAGCTTTTTAATTTCTGTTTCTGTTTGTTTGGTTTTTTCGACCAACATAAACACTGTTTCCGCTTCTTTATGTAACCTAATCGCTAAACCAAAACCACTTAATAAGTTATTGTCAAAACTTAAAAAGCGAAAGTGATCAAAGAAAACGTGTTGGTAAACTGTTTGACTCGCTGATGGTACTAATAAGTATTTAATCTGATTCGACTTAACTAGGTGATTGACAAAATCTAAGTATTCTAAAGCGGAAAGTAATCCCACTTTATTGGTGTTTTGAAAGCAATCGTAAGCTAAAAGTCTTTGCTCATTCACCAAGGTTTGCTCGTTAATGGTTGTTTGTGCCATACCTAGTAAATTTTACCTAGTTTATTATGCTTCAAAATATCGTGCATTGCTTTACAGAAACTTTCATGCTTGCTGTTAAACATTTGGTGTTGACTGTGTAAGATAACCCCTGATTGCAAGTTACCTTTCTTAATCAAAAAGCGGTTAAAGTAGTTTAAAGTAGCTTTTGTAGGCATAAAATCATCATGTTGAGCCAACAGTAAAAAGGCTGGTATGGTAATAGTTTTATAAGCACTGTTTAAGCGCGACAATTCCTTAAAGATCTGAAAGGTGAGTTTTTTGCACATAGCAGTATGCTTGTCTAACTCCATTTGTCACTGTCCATCGTTTTGTAAATCAGGAAACTTTGGTTGAAACATCCGCTTAAAGTAAGACAGCTTTTCGTCCTTCTTTTTAAAAGCCTTTTTAATCTTGTAGTAATTCAACAAGTTGGAATAAGACAGTGGCGCTAAGAGCACTAGTTTTTCCACTCGTTGTGGTTTTAAAAACTGCAATGCATAACTAGCCACTGCCCCACCCATGCTGTGTCCTATTAAAACAATGTTTTCTAAATCTTGCTTTTCAATTCAAGCTACCAATAAATGGGCATAGTGAATAGGTGACAATTCCTTGTCAGTAAAACCATGGTGGTTATGACCCGGTCACAAGGGTGCGTAATAGTCATAATCCACCAGCGTTTCAGAAAAGCTCTTAAAATAAGAATGGTGCACTGAAAAACCGTGCGCAAACACAATAGTTTGTTTAGCGGGTTTTTTAGCTGGTTGAAAAAATACTTCTAGTTGGCTAATATCAACAGTATTAATATTGACTTTGTCTCTCACAATTACCTTTTAAGAAAAGTAAAACACCTTGCACACAATTGGTGTCCTTTTTTAGGGTAAATTTGGGTTTGGAAGTTCCAACATCGTTCACACATGACTTGATCGGTGTGTTCAACCTTAATTTCGTTGGGGTCATTCGTTATATTTACCTGGTTTACGTTTAGCCAAACTGCTAAATGTTTTACAAGACGTTGATCAATTGCACTAGTAGAATCCACACCCAATGTCAATACAACCTGGTTGTTTTTAGTAATCACACCATTTTGACGTGCTTGTTCAATGGCTGCATAAGCCCGGTCTTTAATGGCGCTAAAGCTTTCGTGTAAGCGATCTAACCCTTTAACATTAGCAACTTTAAACATGGCTGGTCCTGCAAACAGGTTTACACTAACCGGCTTTTTAGGCAAAAGATAATTCTGTCAAGCATCTTCGGCAGTATGGGGAATAAAGATATTTAAGAAACCAATTAACTGGGTAAAGATATAGTTCAATACTGCCTGCTTAGCCAACCGATTAGGATTAGTTTTAGCATCACAATAAAGTGTGTCCTTAATAATTTCAAAATACCAACCCGATAACCACAACACAAACTTATTAATGGCCTTTAAGCAATTTAAAAAGTTGTATTGCTTTAACCACTGGTGCAATTCTTGTACCAATGCATTAGTTTTATGGATTACGATTTTGTCCTCTAGAGCAAAACGATAATCCTTCATCTCACCAAAGTTAAAGTGGTTGAGGTTACCCAATATAAAGCGCAATAAGCTGTTGCGAATGCGGCGGTATTGCTCGACAATTTGCTTGATAATGTTGTCACCTATGCGGTTGTCCACTTGTCAATCAACATTAGTTACCCATAAACGCAGAATGTCTGCGCCATACTGATCACACAACTTTAAAGGGTCGACCACATTACCGAGTGACTTGGACATCTTATTACCATGTTCATCCAAGGTAAAACCATGGGAAATCAAACTCTTGAAGGGTAATTGGTCAGTTTGGATAATACCGCAACTGGCGGATGAGTTAAACCAACCGCGGTATTGGTCACTACCCTCCAAATAAAGATCAGCGGGAAAGTTCAGTTTATTACTAATTAAAACGTTGTAAGTTGAACCAGAGTCAAACCATACTTCCAAGGTATCAGTTTCCTTTTTATATTCAACTCCTGGCTGTATTTTCTTTGGGTTTAAAAAAAATTAATATCCTTTTCAAACCAACTGTCAATCCCGTACTTTTTGAGCTGTTGAATGGTGTATTTAATGGTAGTCACATCCAGTAAGGGTTGGTTATCTGCATAAATTAAGGGAATCGGCAAGCCTCATACTCGTTGTCTGGAAATACACCATTCAGCACGTTGCAACAGCATTTCTTGTAGGCGCTCTTTATTCTTGTTATTAACAAACTTAACCCTTTTAATTTGACGCTTTAGCTTACTTTGAATTGATTTAGTTTTAATGAAGAGCTGTTTGGAGGCACGGTAAATCACCGGAGTCTTACTGCGTCAATCGTGGGGTTCGCGGTGCAAAAAGCTTTCACTAAAGACAAAGTTATGGTGTTGCTTTAAGCGTTCAATAATCACATCATTAGCCTTTAGATAAAACAAACCCGTTAAAACTGGATCGTTCAACGTATCATTAAAAACTCCTTTTTCATCAAGTGAAACTAGCGCTTCATTTAACTTGTTTTGCTTGCACAAATAAAAGTCATCCAAACCATAAGCTGGTGAAGTATGCACGAGTCCTGTACCCTCATTACATAAAACGTGATTACCCAACAACACCGGGTTCACTTTGTCATAAAGACAGTGTTTATAAGTAGTGTTTTGTAATTGGCTACCACTAATGGTTTGTACTTGTATAGCATCAGTTCAACCAAACTTAGTCTTAAGCGATTCAAATAAACTGGCCAACACCACGTACTGCTCGTTATTATAAGTAAACAGCAGGTATTCAAACTGGGGGTGAACGGCAATTGCTTGGTTAGTAGGCAGCGTTCAAGGAGTAGTGGTTCACACTAAGAGTTTAGTACCTGAATTTAATACTGCACTCTGCACTACTGTAAAAGTAAGGTAAAGTCCAATTGTTTTTACTTCTTTGTATTCAATTTCGGCCTCAGCCAGAGAAGTGCGTGATACAGGCGATCAGTAAGTTGGTTTTAACGCTTGGAAGATTAAATCCTTTTTCACTGCTTGTAAAAACAGGTCTAGCTCATTTTGTTGAAAGCTTTCATCAATTGTTTTGTAATACTTACTAAAGTCATTCAACAGACCTAAGCGTTGAAACTGGGCTTTTTGAATGGCAATTTGTGACAGGGCAAATTGCTTGCATAAATCGCGCTTTTCACTCAATGATAAGCTCGCATAGTGTTGCGGATCTTTTTTGCTCACCGCATGCTCTATGGGCAAACCATGGCAATCCCAACCAGGAATAAACACTGTACCAAAGCCTTGTAAGTTTCAACTACGGAGCACAAAGTCCTTCAAAATTTTGTTTAAAGCATGGCCAACATGGATATTGCCATTAGCATAAGGCGGACCGTCGTGTAGGATTTTTTGTGGTTTATCTTTGTTTTGGCGGTGCAGTTTTTGGTAAATCCGTTTACTTTGTCAAAAAGCTTGAAACTGCTGTTCCTTAGTGGTTAATTTGCCCTGCATTTCAAATGCAGTTTGGGGCATTAACAGTGTCTTTTTTAAGTTCATTAAGCATAGTAATTTTATTAATATTTAACTAACTTCTATCTGATGTATAAATCAGCGATTAATATCGTCTTATTTTGCCCAGAAATTCCTAATAACACTGGGAACATTGTTCGGAGCTGTACCGCTTTTAAAGCTAACTTGCATCTGATTAAGCCCTATGGCTTTTTTCTCAATGATAAGCGGATGGTGCGTGCTGGGTTAAATTGTTGGGATAAGGTGCAAATGTTTGAACACAAATCCTGGGAACACTTCATTGAAACAACACCACCAAATAAAGCAATTTGGTTGTTGACAAAGAGTGGTACTACTACCCCAGACCAAATTAACATGCAAACAGATAACAGGGAACAACTGTACTTTGTTTTTGGTCAAGAAACCAAGGGATTACCGCAAACACTCATGGAACAATACTCGCAGAACCAGGTACGGATTCCAATTTGAAACCCTGTAAGAAGCATTAACTTGTCCAATACAGTAGCTTGTGTACTGTATGAGTATGCTAAACAAAATCATTACTTTAATTTAGATAAACAGTGCGCTTAAGAAAAGTTAAAGATGCTCTTGTAAGGGTACAAGACAGCCCTTACTTTAGTAACCCAGAACAACTGTCAACTATTGAAAGTGAATCGCTCTTCGTGGAAATAGGTTGTGGTAAAAGTTGGTTTTTAAGCCAGCAAGCACAGCAAAACCCAAAGTGTTTTTTTGTGGGAATCGAGCGTGAACCCACGATTGTTTTAAAGGCAATTAATAAGGTAAACCGCTTAGAGGTTAAACCACGTAATTTATTAATAACGTGTTTGGATGCCAACCAGCTAACAGAGTACTTAAAACCACAATCGATTAGCAAAATCTTCATTAACTTCCCCGATCCTTGACCAAAAAAGCGTCACACGTTGAGGCGACTAACTGCTCCACATTTCCTCAAGCAGTTTCACCAACTTTTAAAAAAGCAAGGTTTAATAGAATTCAAAACGGACAACGATCAGTTGTTTGAATTTACCTTAGAAGTCTTGCAGAAAACAGTAGCTCACTTTAAGATAATTGAGCAAACGACAGATCTGCACAATTCACCTTTGGCTAGTACCAATATTATGACCGAATACGAACAGCGCTTTGTTAGTTTAGGGGTTAAGATTAAAAAGCTAACGCTTGAAAAACTAAATTAGTAAAAATTAGAATAATTACTGCCTTAATGGCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCATTAAACATACAAGCAGCAGATTAAACTGCTGCTTTTTTATTTAGATAACTAGTTTAAGGGTGTGTAAACACTATCCAAGAAATTGAGAAAGTTAATGCTTACCTGCACGGCATGGCGTTTAACGCCAAATGATCCAGCTGAATAGGATTTTTTAGCGGCGGCTGCAGCAGAAGCTGCTGGAGTTGTTGAACCGTTATTTCCACTGTCCTGATTTGGTTGGTTTGAACCTGGTGCATTACTGCCGTTACTGTTATCAGGATTATAGAAATGCGCACTAATTTGATCTAAATTGTTTTTGCGAAAAGCAATCACAATTGCTTTAGTTACATCAGTGCCAACCGTTTGGGCATGAACATTGGACTGGTTAATAAGATTATTGACACTACCGCTGTAAACTGTTTGAATAAAAGTCACAATTTCGCGGTATTGAATGGCAGATTCGTCGTTTCTTCTTAATTTGTCGGAATTGTCCTTATCAGTTTGCTTAGCTAATTCGTTATCACTAGCGATAACTGGATAACGTGCAAACGGATTCCACTTAACAGGGTTAGGATCAATGTCATTCCAAATTAAAAACTTAACTCCTTTAGGGTAAGTTGAATAACGGTTTTGAACCTGCTTAACGGTAGTACCTAGATCACCAGCTCAATTTTCGGTACTAACAGCTTGATTGTTTGAACCATTTAGAAAGTTAATTTGAGAACTGTCAGTTTCAGTGGTTACTACAAGAACGTAGTTACCATCATTGTATTCCGCTGTTCCAAAAACTAAGTTTCTAACCGTTTTATTTTCTTTAGGTAGCTTACTGTAATCAACTTTGTTTTCTACGCTTTGCTGTGAAAGAGTGCGAAAAACATTTGGTTTATCAATATTGCTACAGGAACTCAAAACAATTCCTGAACCTAACAAGAGTAAGGTTGAAAAAGATACTTTCTTTTTAGAAATTGAACTAAACCACTTCTTCATTCAAATACTAGTATTGGTGTCGGAGACCAGACTTGAACTGGCACAGTCTTGCGACCACAAGCCCCTCAAGCTTGCGTGTCTACCATTCCACCACTCCGACGCTGGCGTCTTATAAATTATTAATAGATTTTAAAAATCCATAAACTCTTGATAAGGAAATTTTGCATTTTCTCAACTTTGTGCTTTGTAAATTAATCTATAAAACACAATCTTTTCATCACTTTGACGCTTTTTAAAACCCAACTCAATTTATTTAGTGGGGGCAGGAAAACGAAAATTTTGTGTTGAAGGCCTGATTTTCTGTTCCCAAAAAATGCGTATTTTATTTTTTCATTGTTTTCTAAATTACTTATCGTAAAGGCTTTCAGCACAATTGATGTATCTCTTTTTTTAAAAGAATAAAGGTCATATATTCCTTTCATTCAAATCACTAAAAGTTACAACTTGCTGTTAATTTCAAAATTGTCAAAAAGCCGTCTGTTTTTTTCACCTTGGGCAGATGGTTTTTTATTTGTGTGAATTAAATAAAAATACCAACAGCACCACATCTTTAGGTTTCACTGTTGGTTTTTTATTTCTGAGCCTTGATTATTTGGACTCAAGTTTGTCTATTCTTTTTTCCATTTTGTCCATTTTTACTTCCATCTTGTCCATTCTATTTTCTATTTTGTCTATTTTTCCTTCCATCTTGTCCATTTTTACTTCCATATTGTCCAAGCGATCGCTCATCTTTTGCAGCGTTTGCAAGATTAGCTTTAAGGTTTCGCCCTGAGCTTTTTGCTCGATTTGAAGTTCTTTAATTTGCTCACCCTGGATTCTTATTTGCTCACCTTGGGCCGCCAAAGTGGTTTCAATCTTTATGAGTCGCTGATTGTTAGAATCTTTATATTCCTGAAATTGTTCTTGGGTAACGTATTTAACTTTCACAGGTGGTTTAAGTTTATATTTTCGCAATTCATAAGTTCAAACTCCAACTGTGCCATCACCGTACTCTTCTTTTTCACCATCAAAGTGTTCTTTTCCAATTTTACGGATTAAGGCTTTGTATTCCTTTTCGGAAATTTTTTCTTTCATTAAGTAATACCTCCTTTCTTTTTACAGAGGTTGAGAAAACTACTACTTGCAGCAGTGCTGCCAACTAAGTTCAGCTGTTTTGCTGATTTAATCAATTTGTTTATTAAAGGCATTTTTTGTCCTCCACTAAATAAATTGAGTAGGTTATTTAGAAGTGTCAAGAAAACAACAAATTTTGAAATAGACTAAATACCTTTGCTAATTCTTAACAGTAAGAGAAAAGAAGCCTTGTTTAGTTACTAGCTATCTATTGCCATTAGGACAACAGAAACAGTTTGCTTGCATTTAAAGGATTTAAATCTCTAATAAATTACTTATCAATGACACTGAAAAACATAGCTTTTTTGTTTTTATTACCCTTACTTTTTCCAAAAATTCCACCTTTTTTCTACCTCAATTTTCAAACCATCTGAAAAATAAAAAGTTATTAACAAAATGTTGATAAAGACCTAATTTAGTGCATTTTTGTGCCTTTTTAGTGTTAATAACTTGTTTAAAACTGCTTCTAAGTTCTTTTTGGTAACTATCAGACTAAGCCAGATTAGCAAAAAATGTTTACGAGCCTTATACTGATTAGCGTCTTTGCAGTTTTATGCAGCCGAACTATTACCGTGTCATCAAGAAAGCAACCTCATTTTCAGGGTTAGATATGATCTCTCATGCCTATGGTCAGATTGCTGGCGTTGAAGTTGTGGGTTTTTATTTATGACTAATTACTGAAGCCAATGCTCAAGCCTTTAACAGTGAAATTAGAACGCCTATTTCACGTCTACAAAACGCTTTCAATACTACTGGTACCAAAAACGTTAATGTACCTGATTGAATCTATAAAACCATTGGTAAATTAGAAAGCTTAGGTTTGGTGCGTACCTTCTTTTCACAGGAAAAATCAGAAATGACTTTTTGAATAGTCGAACCATTAGGTTGAAAAGAGTTTAACCAAAAGAAGCACTTTAAAGAAAAGCTAATTGAATCAATGGGAAAACTGGAATATGACCGCAGCTGCCTTTCCTTTGAACAAATCGATAACATTCAGTTTGACAACGCACTGGAAATTACCGCTAACTTTGAAGCTAACTTTACCAGTAAGCAAGATTGTCTTAGCTTTAGTTTTGACTTTGAAGCTTTTCATAAACAATTAGTTAAACAAAATCTCTTTGTTAGTTTTAACCAGAAAACTAAGGCTGTTATTAATGGTTACTTTGAAAAATACCAAATAACACTAGAAGGCATATTGGAATGTGTTATCCCTAGTGTAGTTAATGATGAAGTTGACTTGGAACTGCTACAAAAATTACTAGAGCAGCTAGTTAAAAACAACACAGCTCCAATTGTTGATACTGTAATAAATGACCGCAACTTCTTTTATGATAATCAGAATTTAGCCACAGAAACAAAAGATGCAATTTCCCGTTGTCATTTGGAATACAACGCGGAAAAATACCTATTTCTCTTGTACGGTAAAGTAGAAAACGAGCAGTTAGATCTAATTCGCCGCTTAAGACAAGAGTTTGGAATAGCTGATAAGGTAATTAATCTAATAGTAGACTTCTCCTTTTGGAAAAACAACAGCATGTGACGCGAACAATACATCTTAAAGATTGCTGAATCAGTAGAGCGTTACCGTAGTCATAATAATTACCAAGCCACCCTTGATAACTTTATCCGTGCTTCTAGTTTAGCCAAAAAGCAACGTACCAAAACTAAGATAGAAAAATCCGAGCCTGAAACTAGTGCTGCACCAGTAAATGAAACTGATGACGTCAATTACTTTTTAAACCGTATTAAAGCAATCAACAAGAAGAACAGGGAAAATGGAAAACACAAACGCTAAAACTCAACCAGCAATAAATGAGTTAGATAAGACTACAGTTCAAGACACAATTAAGAATTACGAACAACAACTTCAAAAAGAGCTTAATGAAGAACAACTAAAAGCTTTTAAAAAGCGCTTTAAGTATTTGAAGCTAACTAAAAGAGAAGTGATTGAAAAGGAAAGCAGGGAACTTTTATTGCAAGCATTTAGAGACTTTGAAAATTGTGAAAAGATAAAAGGCAATCATTGTCCTAATAAAGGCAATGCTCACCTCAACATCTTTCGCGAGCCACCTTTTTGAACTAAAAGTAATAAAAAAGTACTGGGTAAGTTAATTGGTATAAAAATGTCCTGCGCTAAATCTATTGATAAGGAGGATATCTTTAAATTGCGCTATAGTAATTTCATCATCTATAAGCCTAACTGATTTAAATACGCTGACGAATTTCCACAAATTAACCAATTACCCATTAACAAATTTCAAGAAGAATCTTTCGAAGAAATAAGAAATGATCTCAGTGAGCTTTATGAAAATGAAAAAAATCACAAGGAAAACATAAGTTTCTTTAACTTACAAAAAGTTAATTTCAATCACGAGGGCGCTAAATTACTACTTTGTGATTTTGTCGAAAGAGGAGTTAAAACTGCGTTGCTTTATTGTGAAGAATTTGTCAGTCGTTTTGACCGTGCATACTGAAAAGTAGATGATTATTTAGATTTAATCAATGAAGCTAATGTCATCATATTTGTCAGTCTTGGTGAAGAGAGTTTTGTTTCCAAAAACTACATTCTCTTCTTAACCAGATTATTCGACATCATTAATTCAAAACGCAAAGATGTTTATTTCTTTAGCACCGAATACAATGAGAAAAATGGTCTCATTCAAGCATTTCAAAAAAGCATTCACTTTAAAGCTAAATGAGTAAATTCCTTTTTTGAACAACTCAATTATTTTTTTGAAATGAAAAGAGAAGAAATTAAATAAAAAGCGTACTAATTTATTTAGTAACGCAAAATTATTTAAAATTAATCGTAATTATTTCCCCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCATAATTAAATACTTAAATTTACACTACGAAATGACAACCTCAACGACCGTTGGGGTTGTTTTTTTATTTATTAAAGGTAGTTTAAGTAACGAACAAGATTCCTGCTACTAATAATTAATAGACAATCTGCGCTTTCTTTCAGTAAAACTTTTGGAAAGCACGCATCACTGGAACACCAATTAAACTCACTAGTGTGAATTTAATCACATTAAAGAGTGAGTATAAAGCAAAGATGCCAACCCAATAATTAGGTACGCCAAATAAAAAGAAGTGAAAATATGGTGTTTTGTTATATATCTTGGCCACTTCGACAAAGTTAGCAGTCGGCACATAGCCAAACCAATACCAGTATAAAGGGGTAATTAACAAACCGTTGAGCGTTGTTAACAGCAAAGTGTTAGATAGAGTAGCCAAACTAAATACCCATAACCAACGAAGTTTAATTTGAGCAAAAATGTGAAAGTACAACCGTGTAAAACTCACGATAGCAAAGTTAGCAATGGTTAGGGTTAAAATACCAATCCAGTTATTACCATCCCAAATAAAGCTACAAAGTGACAGCGCAACGGTAATAACTAAGGCTCAAGAATAAGAAACAAAAAAAGCACAAGCAATTAAAAAGACTGATGAAATGTCAATCGTTAATTTAAGTGCTGACAAAAACGGAATACTGATAAAGTGACTAACAAACGAAGCAATTAGTGCAAGCGCTAGCATCATGGCACTAAAACTAAGGTTAAACAGTTGGTGTTGTTCTTTTTTTGGCCAAAAAGCTATTCTTGCACCATTCATTATTTAGTAAAGCCTACTAGCTAGAAAAGTAGTTAAATAATAATATTTATTAATATGGACAAATTTTTAATCGATGTTACTGTAGAAATTCCTAAGAGCAGTAAGATCAAGTATGAATACGACCGCAAGACTAGTCAAATCCGTGTCGACAGGATTCTCTTTGGTAGTGAATCTTATCCACAAAACTATGGTTTTATCGCTAACACCTTAGACTGGGATGGTGATGAATTGGACTGTTTTATCTTCGCTGACCAAGCCTTTCTACCAGGTGTAGTAGTACCTACTAGAATTGTAGGAGCTTTAGAAATGGTAGATGACGGTGAATTGGATACCAAACTTTTAGGGGTCATTGATTGTGATCCGCGTTACAAAGAGATTAACTCCGTCAATGATTTACCAAAACACCGCGTTGATGAAATTATTGGTTTTTTCAAAACTTACAAATTACTACAAAAGAAAGAAGTAATTATTAAGGGAGTGCAAAGTTTGGAGTGGGCTAAGAAAGAATACCAAGTGTGTGTTGATTTAATGAAGCAATATGGTAAGTTGCCTAAGGATGAGTTTATCGCTAAAATGCAAAAACTCCATCCTGAACACTACCAAAAATAGTTCAAGTTATGATTAGCGCTAACCGTGGCATGTTTTTGGAAACAATTGTTAACCAAACAATTGTACGACTAGGAGATCATCCAGATATTTGACTCGAAAAGCGTTATCTACCGATTCAACCAACTGCTTTTAAAGAAACCCGTGTGCAAGGAGCATTAACAGCGCAAAAGAAATCGAAAACTGACTATTACGGTGTTTATAAAGGCGAAATTTACTTTGACTTTGAAACTAAACAAACTAATAAAAGTAATTTTGCTCTAGCGCAGTTAGCAGAACACCAACTCAAACACCTTAAAAGAATTAATGAGATTGGTGGTATTAGCTTTTTGTTAATCTATTTTCAGGTACAAGACCTCTTATATGCGTTGCATACCAAGGATCTCTTGCAGATGATCGAAGCGCAAAAGGGTAAAAGTAAAACAATTAAGCGTGACCTTATTGATCAAAAAGCACAAAAGATAAATCTAATCTACCCAGGGGTTATAGATCTAATTGATGTAATAGAAAACTTTATAAAAGACTCTTAGTCTGCGTATTTTCAGCGTACCTTAGCAGCCGTTTCCTTAATGGTTTTGCTTGGACTGTAACGCACTAATGGTTTTGGTGGAATGTGTTTCATTTCACCAGTTTTCATGTCTTTTTGAATTCTAGCGTTACGAATCGTAATGCGTAATTTACCCAAATTTTCGGGCAAAATGCAAACGCTACGACTTACTAATTCATTAAGTAAGAGGGTGTTGAGGTATTTAAAGATCTCTTTTATCTTAGCCTCTTTTACTCCAGTAGCTACTGCAATAATCTTGTTAATTTCGCTACGAGACAATGGCTTACTCGATGTTGTTGTTTTTTCCATTAAGTTTTAAGTTCAAAGTTTGAAAATCCAATTTGATTATAAAAACGGAGGAGAATCAAATTAAACGCGTTTTATTTTATTATTGTTCCAATCTTTAAAAGGTTGCACTTTTAAAGGTTAAATTTGACAAAAAGTTTTCCTTATAAGTATGGAACCAAATAATTTAAAAGAAGAGCTAGTTTCAGTCTTTGAAAAAGCTTGTTCTTCACATAAAGAAAGGCTTGATTTTATCTGTTCTGTAAGAGAAAGCGATACCTTTTCCAATGTGGATGTACCGCTAGCTCCTATTAAAACTATTATCGAAATTGCTAAAAACGAGGAAAATCAAACCGAAATTCTCAAATTAGCGATCGAAAACATTAAGACTCTATCAACTGTTGGGTCTGGTCAATACATTGCATCGCACTTCTCAACCCATAACGAAGTTGCTATTATTTTCTGTATCTCTTACTTTTTGTATCACTTCAACTTCTTGCACGATGAAAACAAAAAGCAGTTGCTAAAACGTGCCTTTGAAGCAGTTGCTGAAAAAATAGCTGACTACTTAAACGAAAACTAAGATTTAACCTTATTGGCTACTATTGCCACTTTATCTTTCTCAACTATTACGTTGTAACTAACATCTTTAGTAATAATACCTTCCAGAATTTGCTTGGCAATTAGAGTAGCTATTTGCCGATCGATAAAACGTTTAATTGGTCGAGCACCAAACTGTTCGTCGAAACTAGATTTGTAAATAAACTCATTTAACTTAGGGTCAAACGTGAACTTTAAGTTTTGTTTAGCTAATCTTTGATTTAATTGATCCAATAAATTTGTAATAATTTCTAAAACGGTTGTTTTTTGCAATACGCTAAAGAAAACGATTTCATCAATCCGGTTAATAAATTCAGCACGGAAGTGTTTTTTTAGTGCTTCCATCGCTAAATCGCGTTTGTTTTCCATAATGTAATTGGAACCCAAGTTGGAAGTCATAATTATCATCGTATTCTTAAAGTTAACTAAACGACCTTGAGAGTCCTTTAAAGTACCATCATCTAATACTTGTAAAAGAATATTAGTTACATCAGGGTGTGCTTTTTCAATTTCATCGAACAGAAGCACACAGTAAGGTTTGCGTCGGACTGCTTCAGTTAGTAAACCAGATTGTTCATAACCTACATATCCAGGAGGCGCTCCGATTAACTTAGCGACCGAATGCTTTTCCATGTATTCACTCATGTCAAAGCGAATCATCGCTTTTTCATTATCAAACAAGACCTCTGCTAGTGATTTAGCCAATTCCGTTTTACCAACACCAGTAGAGCCTAAAAAGATAAAGGAACCAATTGGCCGGTTAGGGTCATTGAGGTTTACCCTTCCACGCATAACGGTATTGACCACTGTCTCCACGGCCGCATCTTGACCCTTAACCCGTTTTTTTATTTCATCACCTAAATGCAAAAGCTTTTCCTTTTCGGTTTCTAACAGCTTCTTTAAGGGAATACCGGTAGCTTGTGATACTACTTCGGCCACTTCGTTTTCTGACACTTCTGTCTTAAACAAGTCATGTTTGGAATCAACATACTTTTTCTGTGCTTGTTCTAATTGGTTTTTAAGTTTGGGGATGTCATAGTAAAGAATCTTAGAAGCAGCTTCATAGTTACCTTCCGTTTGGTAGAGCTCTAAGCGTGTTTGCAAATCCTCAATTTCCTTTTTGAGCTTGTTAATGCTTTCAAAGTTAGTTTTCTCCTTTTTTCATTCGGCAGTTAGAGAATCACGCTTTTTCTTTAACTCATCTAACTGTTGTTTTAGATTGTCTAAGTGTTCTTGTTTTTTCTTGTTATCAGCATTTTCCTTATCTTGTTTTAAAGCGGCATATTCAGTTTCTAAGTTAATAATTTCCCGCTTAAGGGTGTCAATTACCACTGGCTCTGAAGCCATTTCCGTTTTAATCTTGGCACTAGCTTCATCAATTAAATCAATCGCTTTATCGGGTAAATTACGATCAGGAATATAACGTGCTGACATTTCTACAGCAGCTACCAAGGCACTGTCAAAGATGGTGAGGTTATGAAAAAGTTCCCAACGGGTCTTCAAACCCCGCATAATGGTAAGTGCTTCTTGTTGACTCGGTTCACTGACAAGGATCTTCTGGAAGCGCCGTTCTAAAGCGCCGTCCTTCTCCACATATTCACGGTACTCTTTCAGTGTCGTGGCACCAATAACCTTAATTTCACCGCGTGCTAACATTGGTTTTAAGATGTTGGCAATGTCCATGGCACCACTACTGGAGTTACGACCTAGCCCCACTATTTGGTGGATTTCATCAATGAATAAAATAATCTTGCCATTTGATTCTTTGACTTGCTTTAAGATACCGTTAACCCGTTTTTCAAACTCACCTTGGTATTGGGTGCCAGCAATTAAACCAGACAACGAAAGTTCATAAATTTCAACATCACGCAAGTTTAAAGGCACGTCATTATTAACTACCCTTCTTACAAATCCTTCAACAATGGCGGTTTTCCCGACGCCTGGTTCACCAATTAAAACGGGATTGTTCTTGTTTTTCCGGCTTAAAATTTCAATTAAGCGTCGAATTTCGTTGTCGCGACCAATAATAGGATCAACCTTATTTTTGAGCACCTCATCGTTAATGCTACGCCCCATTTCCTTTAAGAAATCACGCTTATCTGGTGTTGGTGTAAAACTAAAATCCATCTATTTTATCCCCTTTCTTTGTTAATAGGCCAATTATATAAAATTAGCACTCGAATGGCTTGAGTGCTAACGAGTGGGCTAAAGATTTACCTTTTTTGGTGGTTATAAAGTAAAAGAGAACCAACCCCCAAGTGCTGCTCTCTTTCATTTATAAAGACGGTTTTGAGTTACTTAACTAGTACAACGGAATTGTTTTGGCAATCTTCCAAGTATTAGGGAACAGTGCCTTTAAAAAGTAGTTCAAGCGCTTTTGCACCCACTTTTTGTAATCAATAATTTGCTGTGTTTGAGGCATAATTTCAGTTCACTGAAAGGCAAACAGCAACACGTAAATTACCGTTTCAAAAACTAGTTTTTTGTCTAAGCTACCGTACAAGTCAATAATTTGGTTGGCTGTTTCAATTGTCAACTCTTCGCGAATAATGTTGGCAATCTCATAGTAAGGGTTGTCCACACAAGACCACTCAAAGTCAATTAAAACGACCTGCTTTTTTGGGGTATAAACCAAATTATCAAAGGTGGAATCGTGGTGACACAAGGTTTTGGGAATCGCTTTGTGTTTATCTGTTAATTCCACATACAGTTTTTTGTAAAAGTCAGGTAGCTTGGTTTGGTTAAAGTAAATTAACGGATCGAAGATTCAAATTTCATTAGCAAACTGTTTTCACTCGACTGCATGTACTTTTTTTAAAGAAGCCACTAGTTGTTCTAAAAACAGCCTTGTGGGACGTTTTGGTTGTTTTCCTTCAATCCACTTCTTAATGGCATTACCGGTAGTTACATCAAAGTAAACAAAGGGGTTACCAAAGATGGGGGTCACTAAGTCCAGGATAATTTGCTCGTTGCGTCGGTTCAACGGTACATCTTTACGCGCTAACCTGACTTGGTACTTACTCCCATCTTTCAGTTCTGTAAAAAAGGAAAAATTAGTAAAACCCGAGTGGATTTGTTCCACATGAGCTACAGCAGTTGGTTGGAGGTTTAACTTTTCACAAAGAAAAGCAATTGCATCGCCCTTATTCATCTATTAACTATTTTTGAGTTAGTCGAATGGAGTCTTCCACAATCATTAACTCTTCATTAGTACGTACCCGGTAAATCTTGTACTTACTTTGGGGCGTGGATACCACACAGCTATCACCGTACTTGACAAACAGTTTTAGATCAGTCGCAAAGCCTAATGATTTAACGGCGTTAATAAACAACTCTACACAATCACTAGCATTCTCGCCAATGCCACCAGTAAACACTAAACCATCAATGTTGCCTTCCAATTGGTTCAAGTATTTAGCAATGTAATCCGCTACCCGGTTAACATACATTTTGATAGCCACAGCGTTTTCTTGGGGTTTAGCGAAAACATCACGCATATCCGATGCACCAGTTAACGCCAACATCCCTGATTTTTTATTAAGCGCATTGACCACATCACTAGCACTCATGTTTTCCTGTTCTGCTACATAACCAATAATGGCAGGGTCAAGGTCACCACTACGGGTTCCCATAATAAGACCTTCTAGCGGGGTGAATCCCATTGAAGTATTAAATGACTTACCATTCTTAATGGCACAAACACTCGCACCGTTACCCAAGTGACACACAATCAGGTTCAGGTTTTGCTTGTTCAAGTGCTTTTCTAAATAGTTATTAATGAACTTGTAAGAAGTACCATGGAAGCCATAACGACGTACTAAGTGTTTTTGCTCCCATGAACGTGGTACCGCATACAAGTAGTTTTCTTGGGGAATGGTAGTGTGAAAGGTTGTGTCAAAGACTGCCACATTCTTAGCACTAGGAACTTCTTTGAAAAAGATTTCAATCACATCAGCCTCTGGCTTGTTGTGCAGTGGCGCTAACTTAATAAATTCCTTAATTTTAGCGAGTGCTGCTGCATCTACGATCACCGAATCCTTAAAGTAGTTAGCGCCCTGCACCACGCGGTGACCAACTAGAGTAATGTCACTAAGTTCCTGGATAATTTTGTGCTTTTTAAGTGTGTTTAAAAAGTGGGTTACAGCGGCGTGGTGGTCGGGGAAAGCTACTTTTTCTTCAACCTTCTGTTCGTTAAATTCAAGTTTGAAAAAACCATCGACAAAAATTCGTTCACAAAGGGCTTTAGCTAGGACTTTTTTGTGATAATCAAACAATTGGAATTTGATCGAGCTACTGCCAGCATTGACTACTAAAATTTTGTTGTCGTTCATTTTTATCTAATAGGTAACAATAATAAAATATTAGTAATAAGTCCTCATTTTTTCTCTAATAACTGTCAATGAGTGCTAAAACTTCATATATTGTGGCTGCTGTTTCGACAGCTGTAGGTATTATTCTCTACTTTATCTCTTCCCTCATGGGGGTAATTGGTGTAAGTAATGATAAGACCTTAATCATTGTCTTTTTTGTACTAGCTTCAATCATCATTATTAACGCCTGTGTTTTTTGAATTATTTACCTGTTCAAAAAAAGAAACGTAGTCAAATAAAAAAGTTTGGCGCTTTAAATTCTCCTTACACATTCATTTAGATAGGAGCGAATAAATGTGTAATGAATACTTTTAAAGAAACCCTCTTTTCATTAAAGAAAAGTAATTATTTGTTGAATGAGAGTGCTCAAATTCTCAATGATTGTGTAGTAAATAACAATGAAATCGATGAACGCTTACGCAATTCGATTAAATTTGCCCTTGGCATTATTAATTTAGCTAGTTACTGTCTTAAAAACGAACAAGAACAGCTAGACCATGAACTCAACCATGGTAATGAAGCGCCCTGGGAGTTTAGTTTCAATGAAGCTGAACAACTAATAGAATGCACTGTCAAAAATAACTTTGGCAACGAAAAACTAGTGGATTTAATTTTCCACATTGGTGATGCCATGGAGACTTACCGTACCACTAATATTAAGTTTAGAGTACCCAAGTCTTACTATGATGCCAAACAGCAAATTAGAAAGGTAATTAAAAACTAAGGACTATGATTGCGTCAATTTTTGGAAAAATTACCTTTGTTGGTAAGCGAAAAATTATTGTTGAAGCCAATTGTATTTCCTATTGGTTTAATGTGAAAGAAAACCATAGCTTCGAAAAGAACTTGGAAAAACCGCGCCAGGTCTTCTGTCAGATCATTAAAAGAATGGTTACGAACCAGATCTTGGAAGAGGGCTTTGCCTTCAATACCTTAGAGGAAAAGGAATGGTTTTCGAAGTTCATCGAACTCAATGGCATTGGTAGTAAAACTGCTTTAAACTTGCTCAATAACAACCTCGAGGAAATGCGTGATTATATTAAAAACAGTAACTACCATGCCTTGACTAAGTTAACTGGTTCTAACAGTAAGGTAGCTCGCGCATTGCTGGCACTGGAACTGTATGACCGAGACGATGGTGGTAAACGGATTAAACCCAACACTGCCATGGCCAATGACTATGATGAGATGTTTGACACCTTAAAGTCGTTGGGCTATAAACCCCAAGATATCCAAAACGCTTTGAGCAAAATTGAAATTAAACCTAACTTTGATGTAAGTGAAGTGATTGCTGAAGTGATTAAGTTAATGTCTTTCCAGAACAATGAAGTTACAAATAAAACCGCCTAACAATTTCGCTGAGTTTGTCGGTAAACAAGAAATTATTAACCAAATTCAACTCAGCATTAAAGCGTCCCGTATCAATAAGGCGCAGTTGGACCACATTCTTTTATACGGTCCACCTGGCGTTGGTAAAACCACATTAGCTCGCTTAATTGCGAGTGAAATGAACACCAAACTGCAAATTATTCAGGGTGGGCACTTACAGCGACCGAGTGATTTTCTCAACGCCGTTTCCCTCATTAAAAAGGGTGATGTACTGTTTGTGGATGAAATCCATGCAGTAGCACCCAGTGTGATGGAGTTAATGTTTCCCGTGATGGATGATTTTCGCGTCCAAGTCTTAATTGGCAAGGACTTTAATTCCAAGATGGTGGAAATGAAAGTTAATCCGTTTACCTTAATCGGAGCCACCACTCAGTTCGGTAAGATTATCAACCCGCTGGAAGATCGCTTTGGGATGATCCTTAACATTGACTACTACAGTAACCAAGAGATCGAGCGGATTGTAAGCATCTATGGTGAGCAAATGGAGTTGGAATTAAAACCGGAAGAAATTACCCAGATTACCCAACACAGTAAGCAAACCCCGCGGATTGCCATTCGAATTGTCAAGCGTTTGTTTGAACAGAAAATAGTCAACAAAAAGATTGATCTGGCTGCTCTGTTCAAAAGCTTAATGATTTACAAAAATGGTTTACAGAGCATTGATGTGCAATATCTCAAAGCACTCAACGGGCAGTACGAGCCTCAGGGTATTAAGTCAATTTGTTCGATGCTAGGGATTGATAAATCCACTGTCGAAAACAAGATTGAGCCCTTCTTGCTGCGGGAAAACATGATCCAAAAGACCAAGAAAGGGCGGATCATAACCCGCACTGGGCGTAATTATTTAACTAGCTGCTAACTTTTTGGCACGCTGGATAATCTCATAACCAAAGCTTTGGTTAATCTCATAGATCATTTGATCTAAAGCAAAGTGAGCAGTCTGTTGTTTAGGCTTAGCTTGCTGATACTCATAAAAAAGTAACCCTTCTGGTCTTGAACTGGAGGGGTTATTTTTTAATCCAAAAAAACTCACGCCAATTAAGCGGATGTTAAGCCCCACACCCATTAATAGCCTGTTAAAGAGCTTTTTGACTATTACGAGTAGGGTTTGGTAATCAGCGGTGTATTTCTTGATCGATTGAGAGTGGGAGTTGACAATAAAGTCATTGCTCTTTAACTGCACCACTACTCCCTTGCACATTTGAAAGGACAGTTGTAACCTAGCATACAACTCGTTGAAAATACTAGTGAGTTTTTGCTGGAGTTGATTGGAAGAGTAGTTCAAATCTTCCAGCGTTTCACTTACAGCAATTGAGCGACTTTTGACATCCTGCTCGCTTTGCGTATAAGTTTCACCCAAAGCCACAGCGTGCAAGCTTTCCCACGCAATACCAAACACCCGTTTGAACAAGGTCACATCTTTACACTTAGCGAGGTCTCCAATCTTGTGAAAATTATTTTTAAAGGCGTTGTCAAGTTGTCGTTTGCCTATCCCAGGAAGTTCCACAATTGGTAAGGGTCAGAGTTTGCGTTTAACTTCCTTGCTAAAACAGCTTTTAACCCCAAACGGTTTAGCCTGATTGGAAAAGATTTTCGCTAGGGTAAATTGGTTGGAAATACCAATGGAGATGTGAAAGTTTAACTGTTCATAAACGTGGCGCTGAATCCGTTTAGCCAAACTAAATGCTTTACGGGGTGAAAGGTCACGAAAACACACAAAGCCCTCATCAATCGAAAGGGTGTGTACCTCTAGGTTAAACTGCTCAGCAATCATGGCAAAGATCTTAGCGGAATACTTGCGGTAGTTACGAAAGTGTGATGTGGCAAAGATCGCTTGGGGACACAATTCCAAGGCCTTAGCAATTGGCATGCCCGACTTAATGCCATAGCTCCGGGCAAGGTAATTACAAGTCGACACCACACTCCTAGATGTCCGGTTACCGACAATTAAAGGTTGATTTTTGAGCTCGGGGTTTTCTATCTCTTCTACTGATGCAAAAAAGGCATCAAAATCAAAATAGAAAAAAAGCAGATTAGGATCAGCTTGTATGGTTGGATCTAAATATGTAAAAGTGCCGATCATGTTAATTTAGTTATGTTATTATTAACCTGCAATAAACCAAAGAAAATAACGAGTTTTTAACTTTAAAAAAGTTATCGAGGATTATCATCGTTTGTGCTATTGCAAAAATAATTTCTGATGGAAGCTAAAAAAGATAAAGCTCAGCAGGTTGCTGATGTCTCCCATTTGCTTTCAACCAGTGCAGGATTTGTCATTTTTGACTATACCAGCATGTCAGCAATTGAAGCGACTTCTATTCGCAAGAAGTTGTTTAAGAATGGCAGCAAAATTAAGGTAATTAAAAACAATATTTTGCGCCGTGCTTTAAAAGCTGGTAAGTTTGAAGGAATTGACGAAACTGCCATTAAAGGCAAATTGGCGGTTGCTGTTGGAGTGAACGAAATTGTAGAAACGTTGAAAGCTGTTGACGGTGTGGTAAAGGCGAAGGAAGCGATGAACTTTGTTTGTGGTTACTTTGACAACCGTGCTTTTAACAGTGCTGATTTAGAGAAGATTGCAAAACTGCCAGGACGAAACGAACTTTATGGGATGTTTCTCTCGGTATTACAAGCTCCATTGCGCAAGTTCTTATACGCTTTGGAAGCAGTTAAGGCTGCAAAATAATAAACCCCTCATAAATAATTAATAAGTAAATATAAAAATGGCAAAACTAGATAAAAACCAATTAATTGAATCGTTGAAGGAAATGACCATCATGGAAATCGATGAAATCATTAAGGCTGTAGAAGAAGCTTTTGGAGTATCGGCAACACCTGTAGTAGCTGCTGGTGCTGTTGGTGGTACACAAGAAGCTGCTAGCGAAGTGACTGTGAAAGTTACTGGTTACGCTGACAACGCTAAATTAGCTGTGTTAAAGCTTTACCGCGAAATTGCTGGTGTTGGTTTAATGGAAGCTAAAACTGCTGTGGAAAAACTTCCTTGTGTTGTTAAGCAAGACATCAAACCTGAAGAAGCTGAAGAACTTAAAAAGCGTTTCGTTGAAGTTGGAGCAACTGTTGAAATCAAATAATCAACAATGGCAGTACAACAACGACGCTCAAGCAAACACCGTCGTGACAAAAGACGTTCCCACGATGCACTAACAGCTCAAGCGCTGAGTGTTTGCCAAAAATGCGGTAAGAAAAAACTGTCCCACCGAGTCTGTTCGTGTGGAATGTATGGTGATCTGAGAGTAAAAAAGGCTTATTAAAAGCCTTTTTTATTTGCCTTCAACAGCTGTTACTTGTGTTTTATTTAAAACAGCAGCGTTGCGTGATTTTAAACGACGCGCACGATTAGTAGAAATAATACCTTTACGTGCTAAGCGATCTGCTTGGGAATAAACATTACCAAGGTTGTCTAGGTTAATTTCCTTGTGAAAATTCTTCACGTTGGTCTTTAACTTAGTTTTCTGTCCCTTGTTATTCAGATTACGTTTAATGTTTTGTCGTAAACGTTTTTCGTTCGATTTGATATTAGCCATTTAGGTTTTTTCGCGATTCACAGTATATTATTATCTTACTAATGTAGCTAACAATCATAAATGAATGGTGGTGGACAACAGGGTGGTTTTTTTGGCCTACTCGTCATAATTATCCCTGTCATATTGTTGATTGTCTTTTTCTCTAAAAAGAAGAATTCACAAAAAACAGAGTTTGGTGGTGAAGGGGGCAGCCGTGCTTCCAAAAAAGATGAAGTTTGAAAGACAGTAAAGCAATTTCTCCAAGAACAAAACGAACGCGGCAAGGAAATTATCAAAACTTTTGTCGCTAAGAATCCTAATCCCTTGCACTCCAGGAAAGACCGGCAGTTTTTTAACCAAGAAGTGCAAGCTTACATTACAGCCCATAACTTAAGCAAAACAGCAGCCAAGCGTTACCGTCACGAGCAATTAAAACTCAAACAACGTGAACTGTACTGCATTTACTTCATTACCAAGGACGCTAAAACCAGCGTTTTTGATGAAGCACGCATTATTGAAGCTGAAGTTTACCAAAAGCCCAATAAAACGGGTAAGGGTGCACCAGAACGTTTAATTCGAATCTTAGGATTGAAAAACTTCAATGACGAAATGAAGTGGATTCAACCACTAATGGATCGCGAGGAAAAACGAAAGGAAAAAGAAGAGAAACGCAAGCGGGAATTGGCAGCACGTCAGTTAAAAAGACAAGAAAAGAAAAAACAAAAAACTTCCAAATAACAAGATGATTAAAGTGGTTTTCTTTGGGACATCCACCCTTTCAAAGTGTTGTTTAGAAGCAATTTTTCAAGATCCTGAGTTTGTGGTTTGCGGCGTAGTGACCCAGCCCGATAAGGTAAACGAGCGCAACAATAAAATTAACTTTTCTGCAGTTAAACAATTTTGTATAGAAAACAACATTCCCTGTTTTCAACCAGAAAAAAACATTCAGATTAAAACAGAGTTAGCCCAACTCCAAGCTGATATTGGCGTTTGTGTAGCCTTTGGCCAATACATCCACAACGATATTATTAACCTCTTTCCTTACAAGATTGCCAACCTACACCCCTCAAAATTACCACTACTGCGTGGTGGAGCACCATTGCACTGAACCATTATTAACGGTTTTACGACCTCTAGTTTAAGTGTGATTGAACTCGTACAAAAAATGGATTCTGGTCCGATTTGAAAGCAAAAGGACTTTAAGGTGAATCCCAATTGGAATACCGGTGATCTATTTGAATACGTGCAAACTCACGCACCGCAATTTCTGATCCAGTGCCTTAAGGAAATTGTAAGTGGTAAGAGTCAATGAAAAGAGCAAATTAATCCACCTACTTTTGGTTTGAATATTAAAAAGGAACAAGAAAGACTTGACCTCAATTTGGAACCAAAAGCATTCATCAATTGGGTGAAAGGGTTGGCACCTAAACCTGGTGGTTGACTGGAATTTGAAGGACAAAACATTAAAATTCTCCAAGCAACTTACTTGGGCAAAATGACAAGTACAACCGCAGTTGGTCAAATCACTAAAATTTCTAAACAAGGTATAGAAATCGCCTTAGCTAATGATGAAATTGTTCTACTCCAAATAATTCAAATTCCGGGAAAGCGGGCAATGGGAGTGTCAGAAATAATTAACGGTAAACATCCTTTTGCTGAAGGTAAATTTTTTCATTAATGCTCACCAAGGCCTTTTTTTTAAAAAACTTTGATCGCTCAAAGGAGTTGATTCCTCTGTCTTACGCTGATGTTTTTGGCGCTGCTAGTCAATTGTTAAAAAAACACCATAAGCAGGTCGATACCGAAATTGATGTGCAAGAAGATCTAATTGAAGATCCGGTATTTGAAATAGATATTTTAGAGCTACTCAACGAAGCGCCAGAACTATTATTTGATAGTAAAAATCCTCGGGTTAAAGAGGCACAAATCATTATTGAAAAGGCCAAAAAAGATATTGCTAGCTATTTCCACTTAGACAATATTTTAGACACTGACAACTTAGGGTTAAAAGCCACTGTTACCGAAAAAATCCAATTTACCGAAAAACAAATCGAAGCTGCAGTACAAAACAAAAAAGCAGCGATTATTTTTAAACCAGTGTTTACCGTCAACCAGTGTTTAATCCAACCAGATGCTGTAGTAGTACATGCTAATGGTTTGTGTGAGTTTGTTGTTATTAAAGCGACAACAAATACCAAGAGAAAATTTATCTTGGAAATTATTTATGACTTCTTGCTGTTTGAAAAATTGGGCAAATACAAACTGGTTAACTATTACTTCTGCATAGTTAATTATGAACTGAAAAATAAACACAATGTTTCCTTCTTTTTAAACACCGAAATTAAAACAGCCAAAAACAGTAGTACGAGTAAAACTAAAGAAGAGCAAGTGTTGTACGGGCATCTCCCTTTTAATGATCCAAAAAAAATTGCTTACATTCATAGCAAGAAGAGTGGTGGTGTCAATGGTTTTCTGCTCGTCAAACTAGTGGACAACATTATTAGAAGCGGTGTTACTAATTTGGAACAAATTATTAGTTTTGTTTTTCGTGAACTGAATGCACCCAGCATTCGTAGCTTAAAGCAAATTATTAGTGAAGTAGCTAAAGTACAACTTGATTTTTGGAACATTATTGATGATGTTAAACAACACCAAGAGTTACAAGACAACCAAATTACCTTCAATTACAGTGATGCCTTCAACTCTTTTTGGAATAACTATTTATTGCGCAATCTAATTAAATTAGTCTTTGCTTATAAATACAGTGAAATCTTTCGACTTTCAGGAAAATTAGCTAAATGAGATGAAGTAGTTGAGGCTTATAAAGAAAACAAATCGGTGAAAATTGATGGCTTTTTATACGAACTTAACCAAAGGAAAATGAAAAAAACTAAAAATCCCGCTACTTCCCAATTTAACAAAATCCACTTTTTTCTAAGAGCTTGAAACGATAAAAAGGGGATTGCTGTTGGTAATAAATTTAAGAGTGTTTGACAAAAGCTCAAAGAAAAAAAAGTTTACTTTGATTTTGAAACAATTAGTTCTGCAGTTAGAGTAATTGATAAATCCTTACCCTTTACCCAAATTGTGACCCAGTGTTCTCTTATTGTTGATGACAATACCGAGTCTGATAAAAGCAAGTTAGTTTGTCAAAACCTGATCTTTGATCCGCTAACTATTGGTATTGAGGACTTTAAAACAGTCGTTGATGTCCTTTACCAAAAGCAGTGTGACCAGTACAGTTTTGTCGTTTATAACAAATCGTTTGAAAAGAACAGGTTACTGGAAATGGTTACTTTTATCAATGAAGCTCCATACCAACAAAGAGTGCAAGCAATTATTGAAAATTTATTTGATTTAGCAGATATTTTTGGACTTGAAAATGATTGCTTAGCCTTTAAACAACTTGATGGTTTTTCATCAATTAAAAAGGTCTTGCCAATGATTGATCAACGCTTTTTAGATGCTAGTCGAACTGTTAGTTACCAATCATTAAAGGTACAAAAAGGTGATGTAGCACAAGAGCTTACTTTGGCACGCTTTTTAAATTGTTTAGACGAACAGCAATGAGCACAAACTGCGTTAGAATTAAAGCAGTACTGTGAAAACGATGTACGTGCCATGATTGCAATCGAACTGTTTATTAAGGACTTGATTACGAATCAGCTTTAACCTTATTCTTTAAAGCTTTTAGTTTTGGTTTACGTGCCTTTCAGTTAATCTTTTTCGGTCTAACTGTTAAAGCTCGCATAGGCTTTTGACTGTCTAACTTATTTTTAATGTCACTAAAAAAGTTAATGCCTTTACCACTTTTAAGAAACTGGGTAAAGGCTGACATTGCATTGTGTTCTAACACGCTCTTGTCATCATAGTGTTTACTGTTAGGTACAGCTTGTGACCAGATAATGTCACCGTTAAGCTCAATAATACTCAACACAAGGTTATTGCTAGTTAAACTAACCCGTACCCTAGGTTCTGGTAGCTTTTGTTCTTGAAACAGTGAAAAGAAGTTGTACTTGAGAATTTCTGAGTAAGTTTTTTTAAAAATATGTTCTTCGACAAATTGATTCGCTTTCTCTATGCCAAGGTCTTCGTAAATGGCTGCCACTAACGCCTCTAGTACATCACCAAAAAGTGTTTCAGTATACGGCATTCCTTTACCTAACTTAATGAAATTGCCAAAGTTCAATTCCTTCCCAATACGGTTGAGGTTTTCGCCCTTAACAATTTCAATCTTAGTTCTGGTCAACATACCTTCGTTGTAATTGGGATAAAGCTCAAACAGTTTTTTCGCTACTATGAAATCAATTAAGGCATCACCTAAAAATTCCAATCGATCATAGCTTGCGCGTGCATCTTCATTTTCATTAACGTAAGAAGAATGAACAAAGGCATCTTCGTAAAACTGTCAGTTTTGCGGTTCAATGTTTAAACTCTTGAAAAAAGCGACAAACTTTTCATCAATGACCTTAGGTTTTTGAGTTTTATCTTTCTTATTTTTCATAGTTATCTAAGGAACTTTTAATGGCGTTAATTAGATCAGACTTTAAGCTAGTGTGTGCTAATCTAATGGTGCTTAAAAACTGTTGTTTATCAGCTGATCCATGGGTTTTTAAAGCAAGTTTATTTAAACCGATAACAACGGCGCCTGCGTTATTTTTGTAATCAAAACGCTTAGCAATTCTTTTCAAAATTCCCAAACTAAAAAGCCCAGCCAACGGGTTACGCTTATAACCTTGCTTGAGAAGACGCGCAATTGTTTTAAAGGTGCCTTCCATTGATTTCAACACAAGATTACCGCTGTAACCATCAGCCACTAAGATATCACACACCCCGTCAAGTAAGAAGCGTGGTTCAATGAAACCAGTGAAGTTAAGGTGCTTATCTTCGTTTAGCAATTGGTAGCCTTCCTGGTGGTAATCAAAACCTTTATTGATTTCGGTACCAATGTTTAGCAATGCTATACGTGGACTAATTTTGTTGGTGGTCTTTTTGACAAAAATATCCGCCATTAGGCCCAAAAAATAGAGCTCTTTACCCGTAAAGTTCTTGTTAGCGCCAACATCTAAAAAGTAAAACCAGTCGTTATTGGCAGTTGGTACATAAGACATAAAAGCACTTTTAACATCTTTATGGATTTTGCCAAAGCCATCGTATGTTAAAGAGGCATAAACGGCCGAGGAGCCAGCGGAAATCACAACATCAGCAGCACCATCACGCACAAGGTTAATCGCTTTCTGCATTGAGTTGTTAACCTTGCGCCGTGCGGTTAGTGGTGTGTCGGTCATATCTATGCAAGAGCTGGCTAATTGCTTGGTAATGTTTTTAGGTAGATAATCAAGTCCGTCAAAGGTCTTTTCATCACCAACTAAGATAAATTCCAACTCTTGATGATAGCTCCAGTATTCTAATACTGCGTCAATTGCTTCACGCGGGTGGTTTTCAAAACCTAAACAATCAACTGCTAGTCTAAAAGCCATTATTGTATGCCTAGTATATAAGAAAAAACTTTTTGTTCCCCTGGGGAAAACTCACAGAAAAGCTTGTATTTCTTTTCTATTTTTTCCTTCATTGCTTCCGCTTCCTCAGCGGTGATATCTTTACCATAGTAAGCTAAAAGAAACTCCGGCTTTTTTACCTTTTTCAAAAGGTGGTCAATGGTGTTGAACACGCATTGAACAAGCTCTTTTTCACTAACACAAATGCTGTTATTAGCTACAGCAATAAAGTCACCCTTATTAACTTCAATCCGATTTTCCTTGTACTTTTTCGAAGCTTGAGTAATGGTTGCAGATGCAAAGCCTTTGACAAAACGGCGCATTGTTTTGACATTGGTGTGAATGTTAAGGTCACTGTTAAAAACAGTAAGCGCCGCTAGTGATTCAATCGGGTTGGTAGTCATCACACAATCAGCACTAATCTTTTGGTGCTTTAATTGTTTTAACGCTTCGTTGGCAGAGAGAAAGTAGTTCTTGTCATGTAACAAGAAAATAATGTTACTGGAATGAGTTAGCTTGACTGCTTCTAGTAACGAAAAGACCGAAGGTGCACCGGTGTCATCGGTACACAAGATAGCTTGAATGTCATAGTCTTCGCGAATCCGATCAGCAAAAGCTTCAGTTGGTACAGTCACTACTATGGCTGGTTTAATGTTGCGCTTCACAGGAGCGGGTTTTTGCTTCGCTACCTGTAAATTCATGTTTTCAATTTTGACGAGTTCAAACTCACCATAGTTAAGCCCAAGTTCTAATAACAGGTTAGGTTTTTCGGTATGGGCGTGTACTTTAACAAAGCCGTTATCTTTATCACTAGCAACTACTACCGAAGTGGCAATTTTGCTCACCTTACTTTCAAACTTCTTTTGGTTAAACTTCTGCTTTTCAACTTCTTGACTTACTGACATGCCCAGTTTCAAGACATATTCAGTGCAGTAACCAAACTCTTCTTCCGTAACGTGTTTTTCACCGCTAATGGTCATTTGGCTCAATTCAGCACTGGTGAGCTTACCATCGTTTAAAGTAGCCTTTTCACCATAAAAGGACAACATCCCCTCTAAAAAGCAAGCGAACCCATAAGCACCTGAATCTACTACACCAGAGGCCTTTAACACTGGGAGCATTTGCGGAGTTTTTTTAACTGTTTGTCAGGCTGTTTTAATTACTTGTTCAAACAGTTTTTGCACTGTTTTGGCACGGTTCTTTTGGTTTTTAAAGTCTGTGCTAATTAAGCGAATAACCGTGAGCATCGTACCCTCTACGGGCTTGGAGACATTCTTGTATGCCACCTCTTCGGCCTTTATAAAAGCTGTAGCAAAATCCTCTATGCCAAGTTCGGTTTCGGTGGCTGTTTTAGTGCTAATCATGTCACTAAAAAAGCCCTTCATAATTTGGGAAAAATAACACCAGAGTTACCGCGTGAGAAAAGTAAAAGGTCACGGGTAAAGCTTTTGCCCAAATCACTAAAGCTCTTAATGTGGCTAATTTCACTCTCTAGTTTTTTGAACGCTTCACTCAACGTGACCTTCATGTTTGTCCCCGTATCACCATCTGGCACCGGGAAAACATTGAGTTGATTAATGTATTCGAAATTATTAGCTATATTCTTACAACCAAGACGCAACATGTCAATAAAGTTGCTTAAATTTACTGTAGACATACGAAGTTAAAACCTGTTTTATCTGTGATAAATCGGTTTATATTGCCATTAATTTTAAATTGAAACCCCTTTAATTAAACTAGCAAATCACGGTACTAGTTGGCTTTTAAACTGCCGTTGCGAGTAATTAGCCAGTAAGGGAAATTTCTGCTTATCTAATTGGGCAAAACTTAGATTTGTGGCATGTAAGGCTTGGTGTTTAAAATCGCGGTTACGGTTTTTAAATTGCAGTAATTGGTATTTTCTATCACCAACTAACTGGGTATTCAAGAGGTTTAAGCAAGCACGAATTTGGTGGGTTTTACCACTTACTAAGCGTATAGTAAGCAACGAAAGATCTAACTTAGTTAAGTACTTACTGTTTTCAAAAATGGTGGTAATCGGTTTAGCGTTAGGAAATGGTTTGGCCTTAACTGTTACCAAAGCTTGATAAGCATCTTTCGCTCAATAAGCTTTTTGTGTGCCCTTAAAATTAAATGGGCCAAACACTAATCCTTGGTATTGTTTCATTAACAAGTTATGGCGAAAAACCGCCTCTAACTCCTGTAATGCCTGGTTGGTTTTAGCACCTATTACCAAACCACAGGTATTACGATCAATTCGGTGCGCAAACCGTGGTATGAAGCTAAGACTGTTTTTAAAGTATTGATTATAACCACAGTGTTTCAGTAAGCTGTTAGCTAAATTAAACAGTTCGTGTTTAGCATCGGGTTGACACACTACACCCGCTGGTTTGTCCACCACAATGAGCTGTTCATCCTCAAAGATTACCTGCAAATCATCTTGGACTTGGGTTAAATCCACTACTTCGCTGTGGTTGTGTAAGTAAGGTTCAATTGCAAACTGAAAAACGATTGTATCACCGCTGCTAACACGCTGACCTAATTTAGCCTTCGTGTTATTGAGCAAGACCTTACCTAAGCGCAACAATTTAAAGAACTGACTTAGCTTAATGTTGGGCAACAACTTTAAACAAAACTTTTCAATCCTAACGTTTTCAACAGCTTTAGGCACTACAAACTTAGCTGTTGACATGCTTTTGTTTGGCTAAAAAGTCATCCATAATTTCCACTAACTTCATAAACTGACGCTTGTCATCCAACACAAAGGCAGCAGCATACTTGTGCCCACCACCACCAAACATTTGCGCAAAGTTATTAATCGGAATTGAGCGTGAACGAATGGAACCAATTCACTTCTTAATGTCTTCGTTAAAGTAACAAGTCGTCCAAATTTTGACTCCTTTGATGTTGTTTAAAGCGTGCACCATCGGTAGTGGTGAGACCACCCCAAACTGCTTATAAGTCCCTTTTTTAAGAAGTGCGTAAGCCAAACCATTCGGGGTGATCTTGGCTTTGTTTAAGACATAGCTAAAGTACTTGTGTTCTAAAAGTGGTTTTAGATAAACTGCATCATGAACCTTATTGCGGTTAAATCCAGTTTCTAGTAACTTAGCAGTTAAAGCAAAGGTTTGTGGGGTGGTAGCGGAACTGGAAAAGCGCTGTGTATCGGTAATAATACCCGCATAAATGTAAGCAGCCATCTCAGCATTAAGTTCATAACCCATTTGCAAGATTAAGTAACCGACCATTTCTGCTGCTGCTGAAAAAGCCGGGTCAATCCATTCCAGATCAGCAAAACTCTCGGTTTTAGGATGGTGATCAATCCGCACGGTTTCTTTAGCTAATTTATGTTTTTGGGTTAATACTCGTTCTTGGTTGGAGGTGTCAAAGATAATTGCCAATGAGTTTTTTACAAACGCATCATCAATAGGTGCCGCTTCAAAGGGGAACAAATCTTTCCCGTCAGCCTTAATGTTGTGTGATCCCATCACGTAAGCCTTTTTATGGGGGAAGTAAACCGCTAAAAAGGCCTTAAAGGCAAAAGCAGCGCCAAAGGCATCAAAGTCCGGGTTTACATGCACATATAACGAAAAGTTATCGAATGTCTTGAGTTTATTAGTTAGGTTATGAATAAAATGGGGATCGATGTTAATCATTTTGCAGCTTCTTGACACCAGCTTCAATTAGTTCACGGACAAACAAAAGTTCACTCTCAAGCTTTTCAACGATCGCTAACTTAGCCTTAGTAGTTGGTTTTTTGGGAGCAAACACAGTACAGGTATCCATATGGTGCTCAATTGAAGTTTCAAAGGTACCCAGTGCCTTAGCTCATTCAATTATTTTATCTTTACTTAAGCCTATTAACGGGCGAATCACAAAGGTATTGGGCACAACAGCTTGGATTACCTTCAAATTATCAATTGTTTGTGAAGCTACTTGTCCCAATGCTTCCCCGGTGATTAAACAATCATATTTAAACAAACTAGCAATCTTGTAAAAGCAACGGCGCATTAAAACAATCCGGTATCCTTCCAGACTAATATGACTCAACTCTTTCTGTAAATCGGTAAAGTCAAAGACAAACAGTCTCCCAGTACAAAGCGTTTGGTTAAGGGAAACTAAGTTAGCTAACCGTTGAATTTTGCCTATTGTGGCAGCGTTATGTCCGGGCTCATTAATAAAAGTAATAAAGTCAACATTAAAACCTCGTTGTAAAACTAGGGAAGCGGCTACTGGACTGTCTATGCCACCAGACAGTAACGCCAAAGCAGTACCACTAGTAAAGACCGGTAGACCACCAATACCATTAAAGCTTTCACTAATTATTAGGAAATGTTCCTTAGTTACTTCGACATGCACCGTAATGGCAGGGTCAACTAACTTACCCTTCAATTGGTATTTTTCCCATAACTGTAAAGCTAGGTACTTTTTGAAGGCACTGGAATTGCAAGCAAATAATTTATCACGCCGTCTTACCTCAATTTTGAACGATGGGTGGGATTGTACCACTTGCTGTAGCAGTGCTAACAATTGCGCTTCATCATGAGGGATTTGTAAGGCAAACAAGCAAACACTCAAACCCGGTAATCTGGTAAAGAGATTTTTTAATAACGATCTTTGGGTGGAAATTACTTCACCCACTACCAACATGTCAAACTGGTAGTCAACTGGGTTAGTAATGCTTAGTTTCTTAAAGGCTTGTTTAACGTTCTTTTTGAGTTGTTTGACAAACTGCAAGCGGTTCTTGCCCTTTAAAACAAGTTCGCCATAACGGCAGATTAGAATAGTATTTGGTTCATTATGCAATCCCATTTTCCCTCAGTCACTCCCAGTCTTGTGAGGTTAATTGTGGACTTTTACCAGTACGAATAAAACTGTTGTACCACAGCTGTGCTTTATTAGTTAAAACTTCAAAGGCCTTGCGATCAATTTGTACTGGTACCAAACGGATGTCGCGTTGCTTTGCTTCAAACCATTCGGGATGCACATAGTCTTGTGGTTCTAAAAAAGCAATCGCAAACATTCCTTTGGTAATATTGCGCAAGTACAAATACAACCCAATTTGGCAATAGTAAGCCGGGCTAATCACGATTTTGCCGTTACTGTCAAACCAACTATCTTTCTTTCCGTTAATCTTTTTGACAATCGGCATGCCGTTTTCATCAAACACAATTTTGAGGTTACCGTTAATCTTTTTATAGACCAAAGAATCACAGGAAGTGGTTTTAATTTCTAACATCGGCGCATTGGTATCGTAAGCCAATTTTCCATAAACATCGACCGGTTCCCCATCAGGAATTCCTCCAAAAACTGGATTTTCAGGAAAGAGATCCCACTTAACCTCCTTGGGATCATACGAATGGAAATTAAAACCAGTTTTAGCTATCACATAATCACGGATTTTGGGTTCAATAACTTGCCCCGCGCGTGAGAGTGTTTCATCAAATTCATCCTCATAAATCTTGACCATGTTAGCTCAAGTTTTAAGTGAAGTTTCATACTCAGAAATACCTATTACTTTACCAAATCGGGTACCGGTAATCTTGCGAAAACTACCTTTATGTTTTTGAAAATAGTTGTTTGACAAAACTATTTGGTTGTCAATAATATCAAAATCGATTTTGTACTGTTTGGTAAATCCCATAAATCATTACAAGAGTTGTGCACAAAGCTTTTGTAACACATCAGGAATGTCCTGCACTGTTAAACCTTCATTGTTCTGTTTCTTGATGAAAACAGCACTAGCTAACTGCTCACTGTTTTGCACCAAAGTTTCATCTAAGTTAATGTAGTGCACATCTTTAAGCATTAAGCCTTGGGTCTTAGTGTATTTTTCCAGTGCATTAAATTTAGTTACTTTATTACTTGTAAACAACAGGTTATTGGGATAGAAATTGCTGTGGGTAAAGCGTGGTTGACCACTAGCAATTTGGTCAATAAAAGCTAAGTCCATGTGGTATTGGGGACTAAAGACGTAGATAGAACTGAGTTCCAATTTACCGAGTTGTTTTAAAAAGTTTTCGTACTTTAAGGTTTGTTCAAAGTCCGTTAACCACAGCGACTTAAAGTCCTTGTACTTTTGTAGTGAAATGAAATAGTTAGCTGATAAATTAGCGGTATCATCAACGCGGCTTTTACCCATGGTAAAGATCCCGGAATTGGTAATCACAGCGTGGTGGATAATGACATTAATACTAGCGAGGTCTAAAAAGGCTTGATAGTCATACTGATTGGTTTTGCAAAACCAGGTAATGGCACCTGATTCCGCAATTACATCAACGTTGTTGAGTGAAAGTAAATCTAACAGATACACAATTGTTTGCTGTCAGCAACTCGAAATAAAAACGAGCTGGTGCTTTTCTTGTGCCTTATTGAAAAAATCCTTTCAAATTGCTAGGTCGTTAGCAGCGTCAAAGAAGTTAGCTGGATTAATATCTAGAGCAACAATCTTTCTATCCATCCTTACGCACTTTTTTGACAAAGTTAGCTCAATACAGTTTCTGTACCTTCTCACCTTTAAAGCCACGACAAGTTATTCGAGTAAGATCCTGCATCTCCTTATCGCCGACAATAATTTGCAGTGGAATCTTGTTAACAATGGCTGTGCGAACTTTTTTGCCCAAACGATCTTCGCTCTCATCTAGGCTGACCCTAATGTTTTCCTTTAACAATTTTTGGTACAGTTTTTTGGCAGCCTTTTCGTGTTTATCGATGTTAACCGGAATCACCACTACTTGTACTGGTGCCATTCACAACGGTAGATTGCCATGAGTTTTTTCCAATAAAGCAGCAATAAAACGCTCGTACGTACCGACTATGCCAACGTGAATAATTACGGGAGTTTGAGCATTGCTCTGCTCATCAAAGTAACGCAATTCAAAGCGTTCTGGCAGTAAAAAATCCAGTTGAATAGTGGCAATGGTAATCATCTTATTGAGTGCCGTTTTAAACTGAAAGTCAATTTTAGGTCCATAAAAAGCAGCTGCACCCACATCAATTTTATAGGGCACATTTAACTCCTTAAACACGCTTTCAATTTGGCTTTCTGAGTTAGCTCATAACTGATCGTTGTCAATGAATTTGGCCTTGTTATTAGGATCGTGTAAGGCCAAGTCAATTCGGTCAAAGCTAAAACCAAACTTGTTGTTGACCGTTTGGATTAACTTAAAGGCATTTTTAATCTCAGCTTTAATTTGGTCAGTACGACAAAAGATGTGGTTGTCGAGCAGTGTCATGCACCTTACCCGTTCCAAACCAGTAAGCCCACCCGATGCTTCAAAGCGGTGCAAGATGGAATCCTCACAGAAGCGTTGTGGTAACTCTTTGTAAGAGCGGCGTTTGTGCTTGAAAATCAAACAGTGGTGCGGACAGGTCATTGGCCGGAGCATCATCATTTGGTTTTCTAATTTGACAGAGGGGAACATGTCCTCACTATAGTGCACATAGTGACCACTAATCTTGTACAGCTCTAAGTTAGCTAATACTGGTGAAGATACCGTTTTAAAACCAAACATTAACTGTTGGTAGTGGACAAAATCACCAATAATCTTTTTTAAAACTGCTCCCTTTTCCAATCAAATCGGAAAGCCTGCACCGACTAAGGGATCAAAGGTAAAGATCTCTAAACGCTTACCAATAGCACGGTGATCATTTTGGTATTTTTGTTCATTAGCCGCAATAAGACTTTTAAGCTGTTCTTTAGAGGCTGCAAAAATTCCCACAAGCCTTTGTAGTTGTGGTTTTGTGGGATCACCTAAAAAATAGTTGGCACTAACATTGAGCAGTTTCACAAAGCCCTGTTTGGGTAATGGCAACACTAAATCATGCACCCAAAATGTTTGTTCATTTACGGTGGTAACTACAAGGCTGTTGCTTTCTATACTTTCAATAAGCGTGGTTGTAAACGGATCAGCTTGAAAAAATTTGAGTGATTTTGTCTTATCAATTTTTGTCTGAACAACATTATTTAACTGATTAAAATAAGCATTTAAGTCCTGTTCGAGCTGTTCAAACTCTTTAATGGAAAAGCTCTTTTCCACCTTAAAGTCAAGGTAAAACTCGCCTTCTTGTACCCCGTAACTACCAAACTGTACTTGAGGATAGTTAGCTTTTAATCAGCTTTGTAATAAAAGAACACCAGCTAGCATTAGATCTCTTTGTAATCGATAACTATTTCGTTTTTCGGATTGAAAATCTTGACAATTTCAATTGTGGTCTTTCAAATTTCACTCTTACGCTCTAAATAAGTGCGCAAAATGCCTTCAATACTAATGGTCTTGTACTTCTTGGTGTGTTTCTCCAATTCGTAAGCCAGTTGACCATTGGCATAAATGACGTAATAGTCAGTAAATAAACGCTCGCCAAAAAAGCGCATTTGCTTAATGGTTACTAAAAATCCAGTTTTTTTAACGCTTCAGCACGAACTTTCTATCTCACCCTCCAAAAAAACGCGGTTAAGCATATTAGTTTGGTAATTGTCCAGTTAAGTTAAAGTGAAATTTAAAGTGGTTGATGGTTTCTAACACCATGCGCTCTTCTAGCAAGGCTGTACGCACACCCTCAAACTTTTGTTTATCATCCAAGTATTCGCGCACACTTTGATTGGTTTTTTCGTAATAAAGACTAATTACATTCTTCACCACTTCATCAGTGATTTCCACTTTCCATTCTTTTTGGAGGTGGTTAAACACTAAAGCCTTCTTAATAATTTTTTCAGCAATTGCTTGCACGGTGTTGTCCTCAGCGCCCTGAATCACATCACGCTTTAGACCTTCCATGACATTCTTCAATTCAGCTTCATCGATGTTGAATTCAAAGTAACCCACAATAGCATTCATGGCACAGGAAAAGAGGTTGTCCTTAATGACAATGTGCGTTAGTTCATGATAAAGCGCACTTTCCTTAGCGTCCTTAAAGGAAGCTAAGATCCGTTGGCGGTGTTGTTCAATAAAAGCAGGGTCAGCAAAGATGCGTTCTACTTCAATTACCTTATCGTACTGAATTGGTTTAACTAGTTTAGCGGTTGATTTAAGATTTGTAGCCATATTTAATATCGTTTAAATTGTAATTCTTAAACCAACGCCCTTTTACATTTGTTGTGGTGCAGTAATGTTTAAGATTTGCAATAGCTGACCAAAAACATGGTGCAATGATTGCAGTAAAAGTAAGCGTTGCTGGGTTAAATTGTACTGCGCTGGATCGTTAATTTTGCAAGCCTTATACCAAGAATGAAACAAGGTAGCTGTCTCGTATAAGTAATTAGTTAAGAGGTGCAGTTCATGGCTAACCTGAACTTTTTCCAGTAAGCTCTTAAAGTACACTAACTGGTCTAGTAAAGCAATTTCCTTTTCATGCGTTAATAAGCTGCAGTCATTTACTTGAGCTAGTGCTGCTTGATCCACTACCTTTAATACACTGCACATTCTGGCATACGCATATTGCACGTAATAAACCGGATTGGAAGAGTTTTGCAGGTTAGCAGTGTCCAAATCAATCTTGATAATCGAGTTGTTGGTTTGCGACAACATAAACCAACGCGCCGCATCCTCACTGAGCATTTGCAACATTTGCTTAATGGTAATAACGTTGCCAGCACGTTTGGACAATCTGACTATTTCCTTATTCTTGTACAGCATTACCAACTGTAAGGCAGTAATTTTGAGCTGGCATTTTTGGTGAATCTTTAGTCCCTCATAAACACAGTGCATCCGTTTAATGTGCCCTTCATGATCACTGCCCCAAATGTCGTATAAGAGGTCAAAGCCCCGCGCAGCCTTGTCTAAGTGGTAAGCGACATCGGTACCAAAGTAAGAAGGCCGATTGTCTTGACGCACTAACACCCGGTCCTTATCATCACCATAGAGCGTAGTCTTCAATCAAATGGCACCATCCTTTTCATAGTGCGCTTCGTCCTTAAACTGGGTTAACAGTTTTTTGTAATCTGTTTCCTTAACCACTTGACTTTCAAACTTCCAGCTGTCAAAGTGAATGTGGATTGTAGCTAAGTCTTGCTTAATTACTTCCAAGACTTTGGCAGTACAGTCAGCTAGTAACTGGTCCAGAAAGGGTTGTTGTAAAAACTCTTCAAAGCTGATGTTATCTAAAGGAATACCGGTAATAGTTTTGGCAATACTGTCCACTAGCTCCCCCTCATAACCATCAGGGTGAGGAGTGACCTTAATGTTTTGTTGTAAGTGGAGTGCTTGGTACACACTAAAACCAAACACCCGTGCTTGTTGACCATAGTCATTGACCCAGTATTCCCGAACTGTTTGAAAGCCTAAAAAAACCATCAGGTTATTGAGTACATGACCAAAGAAGGCATTGCGGACATGACCTAAGTGCAACTCACCCGTGGGGTTAGCCGACACCCATTCAATTAGAACCTTAGTTGGTTTAGGGGCAAAAGTAGGTTTGGTTTTTAAAAAATCGAGGTATTTTTGAGGGCTAATTTCAAAGTTAATGAAGTTGTTATTAGCGACAAACACACGGCGGTAATGCGCCTTTTTGTGTTTTAACAACCAAGCAGCAATCTGTTGGGCGGTTTCCTTTTGATTTACACCCTTTAAGTGGAACACAACGGTGGAAGCAAAACCACTAAAGCTGTTGTTTTTAACAAGTTTGACTAACTCTTTGTGCTCATCAAATTCTAGATTCAAAGCGTTAAGACATTCTTGAAGATCTGTGTTAATGAAAAGCATTACTTAAATTATATTTAACCTTGCAAAATGTCTTTTACACTAACAGTTATTGGTGGCGGTCATGCCGGATTAGAGGCCGCATTTATTGCTAGTAAACTAGGGCTAAAAGTTAACCTCTTAGTACTAGATCCCAACCATGTGGGGTCGTGTCCCTGTAACCCGGCCATAGGAGGTCCTGCTAAAGGTATAGTAACCCGGGAAATCGATGTTTTAGGAGGGATGCAGGGTAAGGCCGCTGATGCCACTGCTTTACAATACAAGCTGCTCAACAGCTCGAAAGGTCCAGCAGTGCAAGCAATCCGCGCTCAAATAGACAAAATTGCTTATCAGAAGTGGTTTCGCCAGCAAATTGACCAAACGCCTAACATTGAGTTAATTGCTGGGGAAGCCGTTGATATCCTTGAAAGTAACGGTAAAGTTAAAGGTGTCGTGTTAGCTGATGGTAGTGAACTGGCATCAGATGCTGTTATTGTGACTACGGGAACTTATCTAAAGGCCAAAACTTACTGTGGTAGCTTAAGCAAAGAAGAAGGTCCTGACCGCGCTAAGCGCAGTGAATACCTTTCCACTAACTTAATTAAGCGGGGTTTTAAAACGTTACGACTAAAAACTGGGACACCACCACGGATCCTACGCGAATCACTCGACTTTAGCCAAATGGCTGTGGAAGCTAACACTACCCCTCATTTAGCCTTTAGCTTTACTACTAAGAATTATTTACCACTAGAACAGCAAGTGATCTGTCACTTAATCCATACCAATCCCCAGATCCATCAGTTAATTCTGGCCAACTTAAAACAGTCCGCTGTCTTTAACGGTAGTATTAAAGCAAACGGACCACTTTACTGTCCGAGTATTGAAGACAAGGTATTTCGCTTTCAAGACAAAGAACGGCACCAAATCTTTGTCGAACCTGAATCACTTAGTTTAGAAACGGTTTACCTAGCAGGGTTTTCCACTTCCTTTCCCCCAGAGGTGCAAGAGCACATTGTCCGTTTACTCCCTGGGTTTAAAAATGCCCGGTTTCAAAAGTATGGCTATGCCATTGAATATGATGCCTTTAGTTCGATACAACTCAAATCAACACTAGAAACTAAGTTAATTCAAAATCTGTATTTTGCCGGACAGATTAATGGTACCAGTGGGTATGAGGAAGCAGCTGGCCAGGGCTTAATCGCTGGGATTAACGCCGCTTTAAAGCTACAGCGTAAACCCGAATTTGTGTTACAGCGCAATGAGGCATACCTTGGTGTCATGATTAACGACCTGGTTACTAAGGAAATTAGTGATCCCTACCGCTTGTTAACTTCGCGCGCTGAACACCGTTTGTGGTTGCGCAATGATAACTTACAAGAACGTCTAATTGAAAAGAGCCGAGCCTTAGGTTTAGTGGAAGCAGATGTGTATGCCAATTACTTGGAACAACAACAAAAGAAAAAACAATTAATTGATTATTTGCAAACCACTACCGTTGGGCAAATTGCTGCCTTGAAATTAAACTTTAAAAACACGGCCCAAACGCTGTTTGACTTTACCAAGCGTGCTGAAATTAAGCTGGTTGATTTAGTACAACTACTCCCCAAGCGTTTTGACTTGGATGTACAAAGTTTAAACCAGATTGACATTGACATTAAGTACGCTGGTTACATTAAGAAGAGTGAAAAGTACTTCAAGAGTTTAAACAACCTCAGTAGTGTCAAAATTCCCTTAAAGTTAAATTACCACAAAGTACCTAACTTAGCGAGTGAAGCAATTGTTAAACTCTCCAAAATTAGACCAACTGATCTCAGTGTAGCTAGTCAAGTAGCGGGCATTAACTTTAACGATATTTTAGCGATTAAACATTTTTTAGATAACCATGAATAACAGTAAATTGAAACAGTATGTTCAACTAGTACAAACAGCCAACCAAAACTTTAACCTCACCGGTCTTAAAACTGAAGGCGAAATTTACGAACACTTAGTCCAAGAAATTATCGAGCTCTTTAATGAGTATGACAGTTACTTTGACCATAAAAAGGTTGCAGATTTGGGTAGTGGAAATGGTTGTCCTGGTGTCATCTTAAAGCTGTTGTTTCCCCAAATCAAAACTTTGGATTTAATTGATAGCAAACACAAAAAGGTTAACTTTTTAAAAGAGGTGATTCAAACACTGGAACTAAACAATACCCAAGCACTTTGTGCCAGAATTGAAAACCACACTGAACAATACGACACTTTATGCTCCCGCGGACTTGGCAGCATTATTGAGGTTAATGCCTTTGCTTTAAAACTGTTAAAGCCCAACGGCATTATCTTCCACATTAAACAAAGCTTAGATCAATACCTCGAGTTTGAGGATTCGGAGCAAAAAGACCAGTTTAAACCACTGTTCTTTAAGTTTTTCCATGGCAAGCGCCAGCAAATCCTGATTGCCATGAAGAAAAATGTTTAAGTTAGGTTGAGCTAGCTTTGTCGATCCAGAGTTAGCTAAAACAGCTTTCTTGGAATTTGCCAAACAGTTTTATGTAACTAGTAGTGCTGGTGCAATTCTGTTTGACCTTAAAAAGAGTCAGGGCTTAGACCAACTGCAAGCAATTGAAAAAACAAGGAAGGTTATTATTACAGAACAGTTTGCCAACCAAACTGGTAAATGAATCTTGTTTGACAAGGAAAACACTAAACGGATTAACAGCTTAGCGCAAGAGCACATTACTCCCTTAATAAAACGGATTTTACGCTTAGCGGACTTTAAAAATGTCTTGATTAATGTGCAGCACCACAAGAAGCTGCAAAAATGTCTGTTATGGGAAATTAACGGTTTAATCTGCTTAGTTGAAAGTTTACAGTTCATGGAAAATCCCACTGCAATCATGGAATGATTCCAGGGATTAAAAAACACTGTCCTAATGTAGCAGTTGTAACCATTAGTGGTCAACACAAACCAGTCATAGAACCGAGCTTAACCGAATATAAGGCAGTATTTGGTAGTAGTTTACTCTCCTTTCACTTAGATGCAACTACGATAAATAACTCCCATTTAGTGCGTCAAATTTTGGAACAGATCAAAATAAAAGCTACCTTAAAGTCAAACTCTAAGGTAGCTAAAAGTTAATGGCGCATCGGGTGGGACTCGAACCCACGACAACACACTTAGAAGGCGTGTACTCTATCCGCTGAGCTACCGACGCATTATTCTCTCCAGAGAGAGCAAACCATTTCCAAGGGGCCACTAGGACCCCGTGAAAGTTCGGAAGCGGGGATCTTATGGATGATTAATCCCAGTTCATTGAGAAGATTATTGGTGTAGTGGTTGCGATCAAAGACCACAATTTCTCCAGGGCTTAAGTTTAATACGCCCATACCACAGTCCAAGAGTTCTTTACGCTCAGCCACTTCATCACCACCACCAATAATAACAAAGCGGGGTTGTTTATCAATAATCGCTTCAATTATCTCACCAAAATTAAACTTTAGCGGTTTGAATTTAATTTGTGCTAAGCCATCCACATCCCTCTGGGCGGTGGCTGTGAGTTCAAAGATTTCGGTAGCTTGTAACACTTTGCGCGCAATAATGAACTTGTCGACATCCACCATCGCTATAAGTTTATTGAGATTGGTCTTAGCACCAAACTTGTGGATATTAATTGCAATAATCTGCTTAATTGGATTAGTGTGATCGCTTAATAAATTACGTGCCAAGCTTTCAATTCCCAACACATCGGTGTCATGACTAATACCAATTAGCAAGATTTCATTGCTAATTTGAATAATGTCACTACTGTTAATCAAACAGTTCTTAAAGTCACGGTTAGTATAAAGCTTAACTCCATCAAAACGGGGGTGAAAGCGCATGATGTATTCATACACTAACGAATGGCGCTTTAACTCAGAATCATTAGTGACAAATAAAGCGACACCCGTTCCTAGGGTATTGAAGAACTTATGTGGATAAAGGGAAAACGATAGCGGTTTAATTAAAAACGGTGAATTACTTACATGAATGTCGGCAAACCGTTGCGGGGTAATCGGTGGTACATCTGATACCTTAATGCCACCAAACATGACCTTAATCACATCTACAAGCGAATCTAAACTGCGAAAGAAGTTTAAGCAAGCTTCGAAGGTACTCACACTTTTAATGCCTGATTCCAATAAAAAGTTCTTAATGAAGTCCTGGCGCACATTAGGGTCAGCCGCACTTAAAGCTTCTAACAGTAAATCTTGTAAATAGTGCACCTTTGTACCATGTTGGCGCAAAATCTTAATAAATAACTGGTGTTCCTTTTGAGCGCGTTCTAAGTACATTGCACTGCCAAAGGTCTGGTTATCAATCACTCGTTCGGTTTCGATACCGGGATGATGGGCAATTACTTCCCTTAAAAAACTAATTTCACTAGTAACGCGTACACCACTGTTAAACTGCTTCTTTTTTAACTGTAGTGCTTTAGTGTTTGGTTGATCTAATTGGTTATGCCCATCGGTTTTGACAAGCTGTTTTTTTGACATGTAAATTCGATTAATTTTAAATAATTAACAATTGCTTTAATTGCGTTACTTCCATGGATAGTAAAAAAGGAATTTTAGTGGCAATTGGTGGCGGGAGTTGTTCTGGTAAAACTACGATCGCTGACATGATTTACCAACTTTTGCGTAAAAAGCTTAAAGTTGCTATTCTGCCCCAGGACAACTATTACAAACCATACAAGAACAAATCAATGGCGCAACGCAAAGCGATTAACTTCGATCACCCCGATGCCTTTGATTGAAAGCTGTTGTGAAGTCACCTCGATAACTTACTAATGGGTAAAACAGTGGCTGTGCCAATGTATGACTATGTTAATTACACCCGTAAAAAGGAAACCATAGAAATAGGTCCTTTAAATGTAGTCATCTTGGAAGGTTTAATGCCGTGGTTTGATGAACGGATTGCTAAATTGTGTAAGCTCAAGATCTTTGTGGAAGCGACTGGAGAGGAACGCTTAATTCGCCGGATTGAACGGGACTGGGAACGCGGTCGTGATGTCGCTTCTATCATTAAACAATGACGCGAAGCAGTTAGTCCAATGTACGAAATCTTTGTCGAAAAGATGAAACAGAAGGCAGATTTAATTATTCCCTGGAGTGAAAGACACGAAGTGAGTACTAATGTGCTCGACTTTGCAATTGAACACCTCTTTCGCAAACACGTGGATCCCAACTAGATTTGGAAGGAATAAAGTGGTTTAAAGGTTTTAGGTAAACTACGCTTGTGCTGGGAGCTTTGGCGCAAGTGGTCAACCCGTTGTTGTTTCGTGGCACTTAACTGTAAGTGGCCCTGAAAGTACTGGTCCAATTCCTTATACGTCAAACCCATTTCCGCTTCGTCAGTCTGCCCGGCAAACAGACTAGCAGTAGGGGCTCGTTCAATGACCAATTCAGGAACATTGAAGTGCTGGGATAAAGCATAAACATCGCTCTTTAACAAGAACGCTAAGGGTGCCACATCACACGCCCCATCACCCCATTTAGTAAAGTAGCCCAAGCTGTACTCAATGAAGTTGCCAGTACCCAAAACTAAGTAGTTGTGCTTTTGGGCATGGGTGTATAGACAAGCCATTCGTAAACGTGCTTTGAGGTTGCCGGCTACCATTAACTCCTTTTGGGGATCAATGGTCAATGCTTGCAACATAGCGCGGTAAGGTGGTTCTAAATCCACCTGTTTATTGTTGAGTTGTAATTGTTCCACTAAGGCAGTTGTGGCCTTGTGATCTAACTCGGAGTTATTAATGTGCATTACCAAGGTTAAGTGGTTTTGCGGAAACAGTTTTTTAGCCATGGCGGCTACAACTGCCGAATCAACTCCACCTGACAATCCAACAACCACGCCCTGAGCGTGAGCTTCATTAACGTAATTGGTGAGTCAAGCTTCCAATTCGTTTAAATAGTTCAATAAATTTTTAGTCATTAATTACAAATTCCTGGCCAGCAAACACAATGACATCGCCTTTTTTGGCACCTGCCACCTTTAATTGGTCTTCTATGCCCACTTCTTTAATCTTATTACCTAAACGGCGAATGTTGTCAAGTGTAGTTTGGGGAATCTTGTCAAACCAATAATGTAAACGCTTGCATTCAACATGCCACCGCCCCAAGGCATCACGTTCAATGTTGAGTGGATCATGGTCGGTTTCACTGGCCTGTTCAAAGACATAGTGCTTCTCCATTTCCATGGGTAAATGAAACTTATTGGCACCGAATTGCGCTAGCGTTTTTTGGTGGAGCGCAAACACTTGGGCCACTAGATCACCCAGTTCTTGTTTGAGTGCACTAATTTTGAACAAGGGAATTCCTTTATTTTCGAGGTATTGTGCTAACTTTTGAAAACGGGTACCATCAAGGTCAACGTCTGTTTTATTGGCCACGACCAGCATCTTCTTTTTAACTAACAGCTGGGAGTACTTCGACAGTTCACGCATAATTTGTTCATACGCTTGACAAGGATCGTCATGATCCACGGGATCGAGTGAAATTAAGTGAATTAGGATTTCACAACGCTCAATGTGGCGCAGGAAATAGTGACCCAGACCACTACCCTCACTGGCGTTTTCAATTAACCCGGGAATATCAGCAAACACCAAACTTTGGTCGTTGTGTTTAACTACCCCCAATACGGGCACTAAGGTTGTAAAACGGTAGTTAGCAATCTTGGGTTTAGCGTTGGACAGTTTGGAAATTAAAGTGGACTTACCGGTGTTGGGAAAACCCACAATCCCCACATTAGCTAAATACTTTATTTCCAAGCTCACATGCAAACTTTGACCCTTGTCGCCGTTTTCATACAGATTGGGAGCGCGCATAATTGGACTTTTAAAAGCTGCGTTGCCCTTACCGCCCTTGCCACCGTAACAGAGAATAAAGCTCTGTTGGTCATGAACAAAGTCCATTAAGATCTGGTTAGTATCAAGGTCACGCACCGTAGTTCCCACTGGTACCTTAATAACCAAATCTTCCCCGTTTTTACCATGGGCTAAATCGGGTTTGCCATTGCCGCCACTTTCAGCAAACAGGTGTTTTTTATTCTTTAAAAAAAAGAGGGAATCGCAGTTGTGGTCGGCTTGCAACACAACGTTACCACCATTGCCGCCATTACCGCCACCAGGACCACCCTTGTCATAATGGGCTTCCCTGCGTCAAGCAATAATGCCATTGCCCCCGTTACCCGCACTAAAGCGGCACTCACAATAATCTGTTAATCCCATGGTTGAGCTCGCTTGGTTTTAATTTTCGTATTGCTTATGTTGGTTATTAAAACCAGGTTAGTAAGTTTATTAATTAAAAAGCAGCTTGACATGAGATTGTTCAAACAAAGAGCAGCTATCCCAGTGTTTCAAGTACGGGGAAACAGGCAACCATAAAGGGACCATGTACCAACATGCTTTTTTTGAAAGCTAACTTAATTTGGTTAAAAAATAACCACGGATTTAATTAAGTCCTTCGGTTTATCTTTCATTAAAAACAGGGCTTCTTCAATGTGTTCAAAACCCTTAAAGCGATGGGTAATCATTTTGGAAGGATCGAGTTTTTTGGTCATAATTAAACGTGCTAATTTTTCTAACCTTAACCGACCACCAAGCATAAGACCACCATGAATGGCCTTGTGAGCCATACCAACGCCCCATTCAACTCTAGGAATAGTTAAAAACTCACCAGCGCCAAAGTAATTAACGTTACCAATTTTGCCTCCTGGTCTTAAAACCTTAATCGCTGTTTCTAAGATAGTCGAACCACCACCAGAAATTAACACCTTTTCCATTCCTTCATTGTTGGTTCACTTCAAGATTTTATCCACAACTGAAACCTTTTTGAAATCAATGTAATCAGTCGCACCGTATGCTTTGGCAATTTCCACAATATCTGGTCTAGAATCCACCACAATAATGCGTCCAGCACCCTTTAAGGCTGCACCAGCGATCGCCATTAAACCAACTGGACCAGCACCAAAAACGACAACGCGACACCGTATTGGATGTCAGCTAGTTCATTGGCGTGAAAGCCTGTGGGAATCATGTCTGACAACATACATGCATCAGCTAAATCCATCCCTTCTGGCATCAATGCTAAGTTACCCTCAGCATCATTGACGTGAAAGTATTCAGCAAACATGCCATCTTTAACGTTGGAAAACTTCCAACCACCCAGCATGCCACCGGAGTGCATAGGGTATCCAGCTTGAGCATTAACCGAATTTCATTCTGGTGTAATGGCTGCCACTAAAACGCGATCACCAACCTTAAAGCTTTTGACCTCAGACCCCACTTCATCCACAACACCACAGCCCTCATGGCCTAAAACCATGTTGTGACGTTCACCAATGCCACCTTCCCAAACGGTGTGGACGTCTGAAGTACATGGTGCTACGGCCAGTGGTCGAATTATCGCATCATTGGGTCCACAAACTGGTCTTGGTTTTTCGATTCAACCAGTAGCGCCAATTTTTAACATTGCATATGCTTTCATAAAACTTTATTTACCTCTGCTGTTGCTTTAATAACTGAATTTTAAATAAACAAAAATTATTAAAAGCAAGCGTCTCAAACTATCAGGTAAGAACGCCTTCAAATGGTTTCAAAGAATGGTAAGCAGTTGTAGTTAAAAGCTGTGTTAATAAAACACTGGAGAAAATGGCAAATAAACTAATCCAGCACTAATACAAAAACTGACAATAAGTTATTAATCACTTGATTTAACGCGCTTTTTCTTGATGGTAACAACAACTGTTTTGCTCTTTTCTTTATCTCATTTCGTTTTAATGTCAGACATAGCCTGTTGGCTTTGTTGTTTGACTTTACCTGTGTGAACCAGTCCTTGTGCTACTACCATGGCATAGCGTGAACGTAGTAAAACTAAGTTAGTTAGTTTATTAATTACAAAGAAACTCAGCATTAACACTACCAACACTAACACAAAACGCCCAAAGATTACCCAAACGTTTCAAGCTTGAGGAAACATACCACTCATAAAGAGGCTAATCATCATCAAAGCAATTCCATAAAAAAAGGGAATTGCCTCCACAATTAGGTATGGGGTTGTAAAGAATAAAAGCAGTAAAAGCAACGCATAAACTACTAAGGGCACTAGTAAGAACCACAGTAGTACTAAATCAAAAATGGGTTCTACTTCCCTACCCATTAAAGTACGGTTATTTGGGGCAATGACAAAGTTAAAACCTAAGGCATTAATTTCCCCTAATAACACCCACACAATGATGTAGTAGACAAAGACCAAAAAGAAGCTAAAGCTCCCGGTTAATCAAGCTGGAGTTTTGCGCTTTGCGGTTGATTGAAAAAACTGCTCCAAGTTAGTAAAGTTGTGCTGCTATTTGAAATTTAACAAAGCGATCGTTTTCGTAAAACTGGTAACGTAAAAGCCCTTTAATTACCCAGAGGATAATTGGCAGGCCATAGCTTTGTAGCTCCGCACAATTTTTGTGGTAAAAGCTATCATTGGGCGCTAAGAAACGACAGTTCTGGGTAATCTCATCTTTAGTTAAATGAACTAACTGCTTTTTACTGTTAAAGACAAAACTAGTTTTAAAGCTCTTTAACTTTTCGTTGAGGTACTTAATCGTGGAAAAGTGACTGGACAGCAAGATTAACTTTTCTTTAGCTAACTCAAAACCTTCTAGTAGTTGTGCTAACTGTTCTACTAGTACCTGGTTTTGTTGTTCTTCTTCGCCCTTAATAAAAAGCAAGAGAAAGCGAAACGGGGTTAAAAATTCCATTAACACCTCTTTTAAGGTCAAGATTGTCTGTTCTTGGACATTTACCTTAAACTGCTTCGCTAAGTTAATCTTTTTGAGATCAACTAGGTTAATATCACTCAGTTTATGGGTTTTATTTCCTACCTTATAGTTGTCTAAATTAGTGACTACTATTTGTTTATCTTTAGTGAGTTGCACTTCCATCCACAAACCATCAAAACCAAACAATTGTGCAGCAGCAAAGGCTAAATGAGTGTTTTCAGGAGCAATGGTGCTGTAACCACGGTGTGCAATTAAAAACTGTTTGCGCATAAATTAGTCTTCGCCAAATACCTCCTTAGCATCGGAATCGGAAAACTGATCTAATCATTCATCAGCATCAATCGAGTCCACATCAAAGCTTTCAAACACAGGGATATCCTTGGCTATGTCAATGTTCTTCGCATCTTCAGCGTTGGAACTGTGGAATACTAATCAGTTACCCTCACTGTCAAATTCCCCATCGTACCACTTTCATTCATTATCTTCATAGTAACCATAGTCTTCGTTGCCTATTAACTGCTCCCAGAACCGTGGTGCTTCACTAATCCTTGGTTGATGATTCTGATAGTGTTCATCAACTAAGACTCATTCACCGGTCTCATCATAATAACCGTATCAGATCCATTCTCCTTTACGGTTATATCTACCATAGATTTCATCACCTATTAGATCTGTGTAATCACTTTCACGTGCCCATTCAACACTAGCATCTGGTGTTCATATTTGGTTGTCATCAAAGTAACCAGCTCATACCCATTCCCCACTAGGTTCATAATGACCATAATTGCTATTACCAATCAATAGTTCTCAATCAAGTTGCTCTTCTTCAACGTCATCAACTACTTTTGTTGCTTCAGTTTCTGGTTGAAGTTCTGGAGCTTCAGATGTTGCCTCTACTTCCAAGGTTTCTGGCGTTACCTCCGCTTGAGCTTCTGGTTTAGTTTCAAAATCTGAATTCAAACTTACTTTTGGCTCTGATTGAACTTCGGCTTCGAATTTAAATTCAATTTCTGATTCAGGTTGAACTTCTACTTCTGTTTCAAAATTAGGTTCTGGTTCAGACTCGAAACTTGATTCTTGTGCTAGCTCTTGTTGAACTTCAGTTTCGGATTCGAAATTAGGTTCAATTTCTGGTTCAAGCTCTACTTCTGGTTCAAAATTAGGTTCTGGTTCAGTAGTTTCAATTGATTCTGGTTCAGGAATCGTTTCCAAATCAAAGTTAGGTTCTGGTTCAGGTTGAATTTCTACTTCTGTTTCAAAGCTAGGATCAGATTCAGTATTTAACTCTGAAGTTTCAGCGTCTGCTGTTAAAACAACTTCTTCAGCATTTGCTTCTGTTTGCGCTGTAGCTGTGGATACCCATTTGTTATCTTCATCGAAGTAACCGGTTCAAATCCAGTTTTGCTCTGCATCAAAGTAACCGTATTCACTGTTACCAATTAACGCCTCCCAATCCAGTTCAGCTTGGGCTTCAGAAGTTGCTTTTGACTCGGTTTCAATAGCTGGTTCGACTGCAACAGCTGTTGACTCTTGTTCAACTTGCGCTTCTTCAACGGTCTCTGCAGCTAATGTTGTTGAAGTTTCAACAATTGGTTCTACAGCAAGTGGTGCTTCTTCAACAACTGCTAATACTTCTGGTTCTACTGCTTTCTCAGTAACTGTAACAGTTTCAGTAACAACTGGTGCTGTTTCTAGTTGAACAGTTTCAAGCGGTTCTAAACTTACTGGTGTTTCTGTAAGTTCAACCGTTTCAACAACTGGAGTTGATGCAACATTAGGTTCATTTGATGTTGCACTTACAACAGTTGATTCAACAAACTCGTTTTCTAAAAGTTGTTCTTCAGGTTCTACTGTTGGAATTACAACTGGTTCAAAAACTGGAGTTGATGCAAAAGTAGTTGTTTCTCTACTAGTAGTTGTGACAAACTCATTGACTAGCTCTTGCTCTTGCAACTCTATTTCTGCTACAGTTAGTGGTGCAGTGCTAGTGAGAAAATCAACTACATAATGAAACCCAGCTTGTTGGGGTTGACCGTTGTCAATCACCACCACCTCTTGAATCAGCTCGCTTTGTGGTAAGGCTGTGGTGGCTGTGGTGCCAACCGCCACTAATTCATCAATGAGGTCAGCTTCATTTAAAGCAATGTTAATTGCTTGAGAAGGGGCTTGACTAGCTGCAGCTGCTGTTTGGCTTGGTTTTTTGAAATCAAAACTTTCATTAACAAACTCAACCTTAATAATTGGTTCTTGAAACTGAATCGAACTTGATGCTGGTTGTGCTTGTACAGTAACCACTTGAGGTTGAGTGGTAGTTGTTGACGGTGCAACAAACGGCACTTCTACCATTTCCGTTTCCACTTCCGATTGGTCTAACAGTGGTGCGCTAACACTTAGCTGTTCTAGCTGAGTAGTTTGATTAAATACAGTGGTCTGCTCTTCTATGACTGGCTGTGATGTAGAAACGGTGACAGCAGCAGTTGGTGGTGATGTTTGGTCTACTACAGTTACAGTTGGTGGTGCTGGTAATTCAATTTGTACAACAGGTGTAGTTGTAGTGGCTTTTTCAACCATTGGCAAGGTAAAGCGCACTGGGTCAGGAATCCAATTGAGTTTGGTGTCAAAGAAACCCTTCCACACCCAAATGTTGTTTTGAATGTAACCATAGTTAGGATTACCAACCAGTGGTTTTCAAAAACAGCTATCAATCTGTTTGCGCTTAATAAAGGCAGTATGTTCTTGGGGCAGTTCCACTGTTTCTACCACTTCAAAGTAATACCAGTTTTGGTGTTGATCAAAGAAACCTAATCAAATTCAGGTGTGGTTCTTTCAAAAACCAAAACGAACATCACCAACAAAGATTTGTCAAAAGAGTTGTGCTTTTTCAAAAATCGATTCACGTTCTTGACTGTGGCGGGTGCTGGTTAGAACCTGATTCTGTTCTAAAGCAACCGGTTTTAGTTCAAATTGTTCGGTTTCAACAAGCTCACTGTTGGCATAATGTGCCAGTTGATTTTGACTAATAGCAGTTTTGCTAACAGTCTCCTCTACCGCTTTATTATCACCCCGGTCATCGTTAATAACACTAGTACCAGCTGGTGGCACAATAAAAACACCACGCTTTTTCTCGTCAAAAAAGTCAATAGAAGATTCAACTATCTGTTTTTTGACTTTAGAAGTACCTCTGGTAGCGGTGCTAGCTCTGGTAGTTTTATTCTTCTTAAGTACATTGGTTTTGATCTCCTCTAAGAAGGGCTTCTTGAGTAAATCATCGATGCTAACATCCTTATTTTTAGCTTTAGCAGTTTTCTCTTTAGACTGTTCTAAATACTTTTTGTACTGTAATGTAGTGTTCGATGGGTTCTGTTTTTTTATCGTTTTTGGCATTAACAAACATGCTTAGCACTAAATGCCTTGAGGCGTTTTGCGATTAATAAAATTTTAATTTTTAATATTAATATAACGAACTTTATAGCGCTAGCGACACTAAATTAAAGAGCCATAAGGTGACTTTTTTTGTTTAGGGTCATAAGTCCTTACAAACAATTCCACAAAGACATTGCAGTCATAGTACTCCAGTAGTTTTTGACGCATTTGCATCCCGATCTTTTTCACCATTTGGGCATTACGGCCAATAATAATTTTTTTTTGACTAAGTTTAGGCACACTAATGGAAAGGTGTAGCTTCAACAGGTTTTTAGCTTGATCAAAACTCTTATCGACAATCTCTAGCCTTACTACATGGGGAATTTCTTCGCTGCAGTACTTAATGATCTGTTCGCGCAGCGCTTCTAAAATCTTAAAGTTATCAGTGTCAGCATCTAAAAATTCAACATCTTTACGAAACTCATTTGCTTGTAGTTTCACTTCGTTAAACTGGTTTCAAAAAGAAGTGGCATCAAAATGAAGCAGATCAGTTTCCAGTACTGCTTGCGGTTTAAAATCGAGCACAATTGCTTGCTTGTCCTCCGCTTGCAAAACAACATCAAAGAAGTTATTAATTAACAAAACACGAGTTAAATCACGGTAACGCTTTAACGGTTGTAATTGCGTTTGTAACAAGGGTAACTTTTGCTGTTGATCACTGCGCACCACTAACAACAAGACATCTATCCCACTTAAAGCCTTGCGAATCTCTTGACTAATTAACTCATAGGAGCTGTGACGTTTTTCACTAAAGCCAGGAACATCAATGAAGGTAATGTTTTGCTTTTCATGGGTAATAGTATTAGTGCTAACCGCCAACAACGTAGTGTTGTTCATAGGTGAAACCAACAAGTTATTCGCTTTGTGTAATTGGTTAACGAGAGTTGATTTACCAGCGTTGGTTAGACCTAAAACACCAATTCTTATCGATTCCATAGTTTTAAAACGGTTTCATGAATTTCATCTTGAAAAAACATCGTTACACTTTCAAAGGCCAGACTTTGCTCATGGTCAAACTCAAACAGGTGTAGCAAACCATGGATTAAGTTGCGTGTTAGTAAAAACCAAGGTGTGCAACCAAACTGCTTGGCCTGCTTAAAGATGTGTTTGGGACACAAAAAGATTTCACCAATGGCTGGTGAAAACCCCGCCTCATTTTCGTTGTAACAGATGCTAATCACATCAGTTACCCCTTTGCGGTTGAGAAACTGCCGGTTTAAGCGCTTCATCTTTCATGGTGAAACAATCATAAGCGCAAATTCTGGTTGAAAAGAAAGCTGCAAGCTGTCTTTAATAATTTGCAAACAAAGCTCGATGCCAGCTTCAAAGTTTTTGCCAAAAAAGCGCTTAAACAAGTAGTTAGAGTTAATACTAAAAGACGGTTTCAAACAGCGCTTTGGACGGTAAAAGAATCGTAATATTATACCAACCTTGTTTTAAGTTAATACTTAAATTAACCGCTTGAAAAAGCTGTTGCTGGTTTTCATTTTCTAGCTGCTCTAGGGAAATTTTCTTTTTGTAAAGTCGAAAAGCTTTCTTATCTTCCTTTTTAGTTGTTTCTGCTTGAAAATTAGTTCAAGATACCTGGTTAAACTGGGCTTCATCCTGCTCGTTTTTAAAAACTAAAAAGGTTTGTTCAGACAATTTTGCCCATAAGCTTGTAGTTAGGTATCGTTGTACCTTAACCGTAAATAAGATGGCAACGGCTGTTAAGAATAAAACAAAGAAAGCTAGAGTTAGGCCAATAATTTTTTGCTTCATATTATGTCTGCTGTAAATAAGGACTGAGGTTAATTAGGTCTTGCTCACTGTTTACAAAGCGTAAATCGTGTTCGACCATCAGAACAAAGTTGTTATAGGTCAACGGTTTGATCAAAAGCTGATACACGCGCGGTTTGAGCTCCGTGGCAATGGCGTTGAGTGTTTCATCTAACAAAATCACCTTGTCTTTGAGGTTTTGTAATAAGAACAGTTGCAAGATCTGTTTTTGTCCTGAACTTAACTTAATCTGGTTGTGGATGCCGGTGTCGGCTAACAGCTGGTATACTTGGGGATTTTTACAGTTTAACAAGGCTTCACTGTAAAGTCAGTAATGTCGTTGTAGGCCGTTAACTGCCCCGTTAAATAGTAAACGTTGTGAAACAGTTGGCTTGCACTAAAATCCTTTAGGTCTAATTCATCCAACTTAATTTGACCCTGGTATGACAAACCTCGACCAGTCAGAATTTTCAGCAAGGTACTTTTACCGCTACCATTCTCACCACTAATAACCATGCCATTAGTTAAATTCAGGTTGAGGTTAGCCAAATCGAGATTTTGGTTCAGAGTGATACTACAGTTGTTTAAGTTAATCACTTTGGGACACTTCCAACTGACCTTGAAATTGCAATCATTAACCTTGTCTTCAAACAAGGTAACTAGTTTGTCCTTACAGAACTGGTACAGGTTAACTGCTGCACCAAACTGGATGATTCTCGTGGCATAAGCGGAAAACAATGCCTGAATGGAAAAGATATAAAAGAGAAAGGCTAAGGTGTAGCGCTGTTCAATAATGCCAATAATGCCCAAGGCCAAAATTAAAAAGTCAAATACCTGTTGGGCAAAACTTTTAACACCATCACTCGCTAAGTGGTTCACTTCCTTTTGATTACTAATGTTTTGGTTTTTAATAAGTGCTTTGCGCAACTGCATCATTAAGTAATTGAAGCGCTTGCCGTTTTGTTCATCACGCAAATTGCCATCTAACTGCAAACTGACCTTGTTTTGTAACTCCATTTGCGGAATTGCTTGCTTCGTAATCCGTTTAGTGAAAAAGAAGTCATAAAGAAAAATGGCACAATTCACCACAATTTGGGCAATTGCTATCCATAAGAACTCCATTTGGTTAATACCAATTAAGGTGCCAATAATTAAAGCTACTGTACAGCTAATAATTAAGTTAGGAATATGGACATTAAAGAAGGACAACACTTCCTTGAGGTAAAACTGACGCTCGGTTAATTGGTTAGGTGATTTATTGCTAAAGTAGACAAAACTCTTGGTTTGTAATGTTTGCATAATTCAACCCAAATAGCCCCGATACTGCTTTCAAAACAAAACATTACGCACCATTTGCAACAAGGCATACAAAAGTAAGTACAAGCCTTTTAAAACTAAAAAGAAAACCACAAAAACGACAATGTTAACTGATGTCCCAAAGTCAACGGTGTTGGCAATCATCGTTTTAGAGGCCGTTGCTAAAAGTGTTGAAATGCCAATAATGATTAGTTCAATGAAGACATAGAAAATAATCAGGTTTAAATCAAAGAAGTGCTTAAAGAAATTGCGCTTATTAATGGGCGGAATTTCTTTAAATGATTTTTGCACTGTAGCGGCATAACCAGTTCACTGTTTCTTTAATTCTTCATCATTTAAAAGATATTTCCCTTTAGCGGGGTCAAATACTTCCCAGAGATTATCTTGTTTTTTATGCACCACCACAAAATGCTTAGGGAAGTGGACAATAAAGTATTTACCCTCTAGTTGTTTGAGCTCTTCTAGGGTTAGCTGGTAACTAGCAATTTCGAGACCAAACTTCTTGCCATAGGTCTCTAGTTCAAAGAAACTCAAACCACTAGCTGGTAGGTTAATTTGTTCCAACAGTTCATCATGGGCATACTTATCCTCATGCAGTTCATTGGCTAACATGCCAAGCACACAAATGCCACATTCGTTGGGTTGTTCTTGATAAATAATTTTCATGGGGCTTTATCACTTACATAAATGACCCAATGAGGCTAAGGTGCCAATAAAAAAAGGGCTGTACTACTACAACCCTTCTTTTATCTGCTTATTGAAAGCCTGTTTATTCCTTTGCTTGCTGCGCAGCACGTTCGTATAAAGTACGTACTAAAACCCCTTGACCAAGATCTTGAATCGAAGCTGTGGATGCAATATCACAGTGGATCAAAGGTACCCCTTCCCTAAATTCAGCTAAGAAACATGCCGCCCGGGATGAACCTGCTCCCTTAACACTAGTGGAGTTTTGTAAGTCCGCTAGTTTTGATTCCAATGGTTTTAGGTAATCCGGGTGCATTGGCAAGCGTCATACCGGTTCGCCTGCACAGCAAGCTGCTTTTTTAAAGGCATCCCATGCCATGTCACAGGTACTAAAGATCCCGGTGTAAGTGGTACTTAAGATGTAAGACATTAAACCGGTTAAGGTAGCGACATCAATAATTTGGGTAGCTTTAAGATCCTTAGCAGCATAGCTAATGGCATCGGCTAACACCAAGCGACCTTCAGCATCGGTATTGTCAATTTCAACCGTTTTACCATTGTAAGCGGTTTGGATGTCATCAGGGCGTTGTGCGTGGGGACCTGGTAGGTTTTCTGTTAAAGCAGCTACTGCTACTACATTGGTTTTCACCTTGTTTTTCGCTAAGGCCAACACGGTGGAACACACAATAGCTGCCCCACTCATGTCGTACTTCATACCACGCATGTAGTCACCGGTCTTAATGTTCATCCCACCCGAATCATAGGTAATGCCCTTACCAACTAAAGCTAAAGTTTCACTGCTCTTCTTATTATTGTTATAGGAAATTACTAGTAAACGCGCTTCACGTTCCGAACCCTTGTTAACCCCTAACAGTAAGCCCATCTTCTTTTTGATGAGATCAGCTTGCTTTAAAACGGTAATCTTAACACCTAAACCAGTAGCCGCCTTTTCAAAGCGCTTCACAAATTCCTCTGGGTAGAGTTGGTCAGAGGGGGTGTCTTGGAGTTGACGCGCCATGTTGACATAGTTACCCACTACCTCGTATTCTTTAATGAGGCTCGTGTGTTCGCTGTCACACTTCACTGCTACTTCCACATTCTTTTGTGGTTCGGTCTTCATGGTGAAAGGTGTTGTTTCACAGAAGGTAATGCTAGCTACCACTACCTTTAATGCTGTTTTGTAACAGAGGTTTTCTGCTTTGTAGAGTTCCACAAAACTGTCTAAGTCCACGTTTAGTTGGTTCTTTTGCGTTTTCAAAAAGGTAACTAGAGCGCGGTTGAAAGCGGGGAAATCAGTCGGAATTTTTACGCCAAATTCAATTTGGCCTTCAACACACTTAGTCTTACAGCAATCACTCTTGCCGTACTTATCGGACTTTTTAAAAACAACCGTCTTGCTGTCAAATAACTTATTTAATTTCATCTTTAATTTTTACCTATTCTTGGAAGCTACCTTCCTTGTTTTCCTTAATATCATACACAGCAACGCTGGTGGTAATTAAGGAACTTGCTACTGATGCAGCTTTTTCGAGTGCAGTTTTGGTTACCTTAGTAGGGTCAATAATACCGCTGCGGATCATGTCGACCTTCTTTTTGGTTTCAGCATCAAAACCAACACCATCAGCTTCACTGCGTAGTTCTGCAAATACTTTAACTGGGTTAACCCCGGAGTTTTCAATAATTTGGCGCACTGGTGCTTCTAGTGACTTACGCACAATTTCATAACCAACCAGGATTTCCTTGAGGTTTTCCGCACTAGTTTCTGCCTTGTACTTGTCAGCCAACTTACTGTCATTGAGGATAGTGGATACGTTAAGTAAAGCAATACCACCCCCGGAAATAATCCCTTCTTCAACAGCTGCCTTAGTCGAGTTTAAGGCATCTTCAATGCGGAGTTTTAACTCTTTTTGTGCTAGTTCCGTAGCACCACCAACCCGTACTACAGCTACCCCTTGACTGAGGTGGGCAATCCGTTCCTTTAACAGATCGGTATCGTACTTTTCGGTAGTTTGCTTTAAACGACCATTTAGAAGGTCAAGGTGTTTTTGGATCGTTTCCTTAGAACCCTTACCACCAATAACCGTGGTTTTTTCTTTGGCTACTTGCACACGGCGAGCTTCCCCTAAGTGGTTTACAGTCACATCCTTAAAGCCACCACCTAGTTCATTGTTGTAAGCTAAGGTGCCAGTACTAATGGCTAAATCTTCCAAAGCTGCCTTTTGGCGTTCCCCGTATTCGTTACATTTAACAGCAACTACATTAATGGTACCGCGAAGCTTGTTCACCGCTAAAGTAGTTACCACTTCTTCAGCAAAGTCAGGTGCCACGATTAACAGTGGGTTACCGTTTTCCATGCTACCTTCTAGTAACGGTAAGATTTCCTTAATGGTGTTAATCTTCATCGCACTGACCAAGATCTTCGGTTGGTCTAACACCACTTCCATTTTTTCTTGATCGCTAACCATGTAAGGGGAAGCGTAAGTACCTTTAAACTCAATCCCTTCAGTGGTTTCTAGGGTAGTGTTAATCGTCTTAGCATCATCGGTGGTAATGACACCGTTTTTACCAACTAAGGCCATAGCTTGAGCAATCAGCTTCCCAATTTCCTTGGAACCGGAAGAGATCGCTGCAACTTGCTCGATTTCTTCGTTGGTATTAATCTTTTTGGAACGCTTATCTAACTCAGTAATAATTAACTGACTAGCATCTTCAATCCCACGGCGGACATTAACTGGATTAGCGCCATTATTAACAGCTTCCACACCACGGTTAGTCATTTCCTGTGCCAAGATGGTAGCAGTGGTAGTACCGTCCCCAGCAATATCATTGGTAGATACAGCAGCTACCGAAATAACCTTAGCACCAATGTTTTCTAAAGGATCAGTTAGTTCAATTTCCTTAGCGATGGTCACTCCATCGTTAGTAATTAAAGGATTGGAAAACTTCCGACCTAAAATCACGTTTTGACCTTTAGGACCAACGGTTACCTTAACAGCGTCTGCTAATTTATTAATACCAGCTAGCAGTTTATTGCGGGCATTCTTGCCAAATACTAATTCCTTTGCCATAACTATTTTTTCTTAGCTCCTTTCTTTACAGTAGTAGTTTTTGTATTACCATGCTTTTCAAAAGCAAGGACATCTTCCAAGGAAATAATCTTGTACTTGGTACCTTCCTCTTCAAAGGAAATACCACTGTATTCCTTAAAGTAGATCGTGTCACCGATGCCAAAAGCATATTTCGGTTTTTCGCTTTTACCATATGCCGGTCCTGCACCTAAAGCAACGACAGTTCCCTTGTGGGCACTTTCATTTTTATCAGGGTTAGCTAGTGCTGTAATAATCCCTTTTTGTGACACTTCTTCTTTGTTTGATTCCACTAGTGAAACCAAAATGTTGTCATGAATTGGTGTTATCTTCATACCGTCAATATTCTACCAATTATTAACAATTAGCAGTTAAATGCTTAAAGTGCAAATAAAAATAATTTAGCAACACTACCGAAATAGTGCTGCTAAATTAGCGTGCCTGATTTATTTACTAGTAGATTAAGCGTTTATTGCCAATAAGGCACTCAACCTTGTATATGTGGTTAGTAATTAAAACTAATTAAGAGTTATTTTCTTTTGGAAGTGGCAGACTAGCATCAAGCACACCGAAAGCAATTAACATGACTACGGCCACTAACAACAATAAGCTACCGACAACAAAGAAAGCAACAAATAGATTTTTCGTTTTTGCATGAGCGGGACTACCCACTTCAGCGTGTTTGTGCTTATGTAGGAAGATGCCACCCAACAAACAACTCAAGAAACCAATGATTAACAACAAAATAACTAAAAGGCTAATTCCACCAGCAATTTGGAAAATGTTACTCGTTATTCCTTCGGCTTCATTGAAACCAGGAGTTTTACCACTAGAATTCTGGGAACCAAGCATTTGCTTTTGGAAGAAACGCGTAATAAAAGGAAAGCTTAAAGCTAAATTTTGCAGCATGAAAAATATTTGTTTGGAAATAACAAATACTTAAAACAACTAAAAAACCAATGCTGACAGTAGTAACGTTAACGCTAGTTAATCTATCGTATCTTGGTTAGGAAATTTAATTGAAAAACTACCACAAACTTTTCAACACCCATTAAAGGCTTAAAAAGTTTCAACAATAAAAACTAAAAACTCTACGCCATCACTGGCATAACAGGAAAATTTAATTTTTAAGCCTAATGCTAATTTCTTTTAAACCCTAAAAAATTAATTGGACTCGCTAATGAAAACGGTTTTAACTCGATAATCTAATGGTTTTATTACCTCTAAAGTGACCAAGCTTCTAATGGTTTTGAACTGTTTATTTCAATAAAAAATCACAGCATCTTTTAATGAGTGAAGTGATTTTTTTAAATGCTGTATGTTTAGTAAATTAAGTAGTTATCTAATACTCCAATCCCTTATACAACGGAAAACGTTCTAACAGTTTTAAAACCGCTTTTTTTACTTCTTTAATAACTTTCTTATTACCGTCAGATTTAATAATCTTGTCAATTAAGGCAGCAACATAAAGGAAGTGCTTTTCCTTAAAGCCACGAGTAGTCATGGCAGGTGTACCAATACGAATACCGGAAGGATTTATGGCAGGATTCTTATCGAAAGGAACCGTGTTCATGTTCAACACAATGTTGGCTTGTTGCAATCATTGTGAAACATCTTTACCCTTACCGACATCAAGCGAGAACAAGTGGGTATCAGTTCCGTTAGAAATGACGCGGTAACCTTGTTGTTTGAACCAACTAGCCATGCTCGCTGCATTGGTCTTAACGTTTTGGATGTACTGCTTGTATTTTGGTTGTAAAGCTTCTTTAAAACAAACATACTTAGCCGCAATTACGTGCTGTAAGGGACCACCTTGACAACCCGGAAAGACACCGCTGTCCAATTTGCGAATAATCGCTTCGTTATTGGACATAATTAACCCTCCCCGTGGACCGCGCAGTGTTTTGTGGGTAGTAGATGTAACCACATCAGCGTAAGGTAAGGGATTGGGGTGCAAGCCAGCTGCTACCAATCCCGCAATGTGGGCAATGTCCGCTAAAAGGTAAGCACCAACTTCTTTCGCAATTGCACTAAAGCGCTCAAAGTCAACGGTTCTCGAGTAATTGGAAAAACCGCAGATAATTAGTTTAGGTTTTACCTCCCGAGCAACACGTAGCACCTCATCATAGTCCAACATCTCGGTTTCGGGGTTGATGGTGTAGGAATAAGGCTGGTACTGTTTACCCGAAAAGTTAAATTTATTACCGTGAGTTAAGTGCCCACCACAGTTAAGGTCAAGCCCTAAAATGGCATCGCCAGGTTTAAGTAGAGCTAAGTACACGGCATAGTTAGCAGAAGAACCGGAGTGCGGTTGCACATTCGCTCACTTCGCACCAAATAATTCCTTACAGGTATTAATGGCTAAGCTCTCTGATTCATCAACTACCTCACAACCTTCATAAAAGCGTCTAGTAGGATAACCCTCAGCGTACTTATTGGTCAAAATGGAACCAGTGACCTCTAGGATGTCCCTACTCACATAGTTCTCTGATGCTATTAAACAAATGCAATCTCTTTGTCTTTGCAATTCCTTATTGAGGATTCTTCTAATCTTTGGTTCCATGGTCTTACTAATTTACTTTAATTTTGATCAACATATTGAAACTGTCGATTGTTACTCTTTTTAATTTAATCACAAAGATGAGTGTAGTGTTGTTTGGCAAATTGGGTATAGTAATGGGTAGTATTGCGGTTGCCAAAGATAAGATCATAACCAATGCTGCTCTAGTTCGGATTGAGGGAATTGCCCTCACCCTGAAAGCGCAGCATTTGGACTAAACCAACATCGTTACGAACAGCCTTCTTGGGATTAGTAATTACCGTAGCAAAATAGATTCCCACAATTTGACGCTTTTGGTTAATCACTAAAGAACTAGAAGTCCCTTTTCTTAGGTTAACCTGATCGATTCCTAAACCATGACCATACATCTGGTAGTTTTGCCCTAACCACTTATTCTTAATCTTGAATGACTTAGTGTGATCAAACGGTCAAGATAACTTACTACCAGAAAAGTGTTTTGTTTTAATGGTGGTAAGTTGATTGGTAGTCAAAGTAGGAATTTCGCGGTGGTGATCTACTGACCAAGGCTCTTTAAAAGTAACTTGAGCAACATCCTCTGGGCGAGTAGGTTTAACTTCAGTTTGGTTCAAGTTAAGTGCAGCAATATTGCTCTCTACTTGGGGATAACCTAAAGCAAAATAGCTATCTCGCTTAAAGCTTGCCATAGGTTGGTAAGCAAAGATTCCCACACTGTTACCTAACCGTTCATAGGTATTGATGGCGGGGCGCATAAAGTGGTCATAGATCTGTTTATCCATTTGGTTGTGTAAAAAGAGTGGAACTTCTATGATCGCAAAATCCAAATAGCTTTTTGCTAGTGCAGGATCATCTTCTGATGCACGAACTGGTGCTACCCACTGGTTACCGCGGTTTTGTAAGGGCACAAAGTCGCGTGCTACAAAGGCTGTTTTGGGAATAGTGGAAGTTTGTATTGACACAAAGGCGTTGCTTACATCAGTACGTAGCTTAAACAATTGAGGGTTAATATATTTCCCTAGACAAAATACCGTAGTTAAACTGTTGCCAATGCTAGCTTTATTAAACGGTGCATAGTCCTTCGGATTGCTCAAAGCAACGGCAATATGCAGGTTAGTCGCTAAATAAAGATAAAACGGTTCGCTCAGGTTATGTGTTCGCTTCGGTTTCTTTCAATCAATTAATCAACCAGTACCAAACACACGTCACTTGTTGTTGGCAAGCTGCATTGAAAAGCTCAAATCATACAGGTCGTGTAAAGCTTCTGACTTTTTGGGAATAGTATTAATGAGGTAATTAGTCTTTCACTCTTTACGTTTAACACCCCCCGCAGCTGTAACGGTTTTCAAAAGGAACTACAGCTATTTAGGGGAATTGCTACTAATAAAAGGGCAGGTAGCAGAGTTGACCTACGAACACGAAACGCAAATTAAACACTTAAGTTAGCTTAAGTCTAATATTTAAGTGAAAACAAGCCTTTTTGAATAAACACAGATAGTTGCAGAGGCAAAACTGACTTAAACATTCGCTTAAGTCAATTCAAGAAATAAGCAGTAAATGAGCGAATTATTTCTTTACTTCTTTTTTGAGTTTAGCCACAACCTTATGTTGTTTGCACGATAGTTTAAAGCAAACAAAAAACCACTAAGAAAAAACGAAAAAGAGGATGCTCAATTCCAATAACCACCAAACGGAGTGAAAGTGACCCAGACTAATACAAATAATGGAAATAGGCAACTCTGCCATGAAGAACACAATGAGCAAAACGTTGTATATGTCTTACAACTTCATCGCTCGGACTAGTGGTAAAACTTACCATGCTTCGGTTTTGGTAGTTGATTGTTCAGTTAACGAAGGTTGGTTGTGGATGAGGATTTGCCACAACTGCTTGGGGTGTTTGTGTACTGGGTATTGGGCTGCACAACGAACATTTGCAACTACTACCGGTCTGAGGTTGGTTTGGCTTTAGTTTCTGGTTTCACCACTATTGGTTATCTTTGTGGATTGCTGATTGAGTATCAGTTGGACTTGTGATCAAAAGGCCTTCAAGCCGCAAAAACTCTTCTTCTTGGTTAATGTTTTTGCGCCATGTTTCCAAGAATCCCAAAAAGACTAAGACAAAGATAAAGGCAATAACAACTAGGGCAATAACAAGGCTAATTGGCAGATAATATAGAAAAAACAGTGAAATAAAACCGAGTCAGATAATTAACGACACAACAAACAATCACAAATAACACTTGTGTAATTTTCGCGCTTTATTGAGATCACCAGTTTGAAGTTGATCAGTATTATTTTGTTCTTTGTTCTCCATTCAAAATTTTCCCTACATTATTTGTTATCTACTATCCTGTTTGAATTTCCAGATTTACGAACGCGTAATTTTAGCTAAGAGCTTTAAAAATAAGTCAAAAATGGAACTGTTAAACAGTTAAATTACAACAAAAAATAACCGTTACGAAACCATTGAACAGGACACATTTTCGCGATCAATAAACTTAATTAACTGGTCATTACTTTGTTGAATTTGCTCATACAAATGGGTGTGGTGTTGCTTGGCAAATTGGGTATAGTAATGGGTAGTATTGCGGTTGCCAAAGATTAAATCATAGGGCACACAGTTGTCCTTTACCGTCGCTGTAGTACTTTCATCAACTGGCAAGCGTAACAGTTGTACCAGAGTTTTTTCACCAGGTTTAGGGTCGGAATGACTAGCTACTGCAAAAAAGATGCCTGATATTTGTTGATCCTGATTGAAGACTAAACTACCAGAAGCGCCCCTGCGGATATACAAATCATTTAAGGCTAAGCCTTTACCATACTGTTCATAGGTTTGCTGATTAAACTTTTCGTCTAGTTGGTAATACTGGGCGGTCATACACGTGTGCTAGTGTATTGCCAGATGTTTGCCCCGTTTTATTCATTACCAAGGTTGGAATCTGACGCTATTTGTTCAAAGTTCAGAAGCTTTGGTTTTTAACTTTAATGCGGTCTTTAACCGGAACACTAGTTAAAGTGGCACCTGTTTGGTTAAAGTGAATCGCATCAATGTTACTCTCTAGTAAGGGATAACCGAGAGCAAAATAGGTGTCATTTTGTAAATCAGAAGATGACGTACTTGCAAACAAGTTTGTACTATCACCTAAGGCCTTATAGGTAGCAATGGCGGGTTTGATAAAGTTTTCCCATACTTGGTAATCCAACTTGTTGTTTAAGTACAAGGGTACTTCTAACACGGCAAAATCAGCATAAGGTTTTAAACCAACTTCCGGACAAATTTCATTTAAGGATTTCCAATGTTTATTGTCAAACCGATAATCAACAAAGTCCACCGCTGCATAAGCCATCTTTGGTAAAGCTGCTAGTTGGACATCAACAAATGCGTGTTTGACATTCGTGTACAGACCAAATAAACTGGGATCAGTGTATTTACCAATTCGAAAACTAGTGGTTAAATCTTGGGTATTATCGACTTGGTTATAAGGCGGGTAGTCGTGCGGGTTCTTTAAAGTGTCAGCGACATGGAAGTTAGTCGCTAAATAGGCAGTGAACTTATTTTCGTTTGTACTAGTATCCTTTCAATCAATTAACCAACCAGTCCCGTAAGTATTTCACCGTTGTGACGTTAAAGTTAACGAAAAGCTTAAGTTATAAAGCTGGTGGTAAAAGTCAGTTGGTTTGGGAACGGTATTAGTAGCCCACTTTGTTTGGTATTCAGCGCGCGGTACCTTTTCTAATTGCACAATCGTGGCACATCCACTAAGCGGTACGGCAAATAAACAAGACTAGCACTAACAGAAAAACGCCACATACACAAACCTAGACAATTTAATTTAAGTATTTTTTACCTTTGATTCGTTATCTACATATTTTAAACTAGGGTCTCGCGCAGATTTAATTTGCCCATAAAAGTGAGTGTTATTTTTTTTGCAAACTGGGCATAGTATTTTTTGGTATTGCTATTACCAAAGATTAAATCGTAAGCAACACTGTCTTTTTCTTCATTAAGAACACTTCGCGGTTTTCAGCGTAACAGCTGCGCTAGACCCCATTCTTGTGTTTCAGTGATTCTACTAGCAAAGTAAATGGCCACAATCTGTCGCTTGTTATTTAAGACTAAGGAACCCGAAGCGCCACGACTTAAACTACCATTAGTGATCCCAATTCCCTTGCCATACATTTGGTATTCTTTGCCACGAAACTGGCGATTAAACGGAAAGTTCTTGGTGTTATCATGGTTTCAAGCCCAGGTGCCACCATTAAACTTGTCACTTTTAATCCTACCCAAATGCGGTCCTTCTTCGTTAATTGTGGAAACAAAGGGCTGATCATTGGGTATTTGGGCTGTTTCACCAGGCACTGTTTTTTCTTTCCCGGTTTGGTTTACAAATAAAGTTGGCAGCTTTTTCGCTATGTAAGGATAGCCCAGTACATAATAATGACTATGTTTAAACTGGTCTAAAGTTGGGTAGGCAAAAATGTTTAAGCTATCACCTAACTGTTTGTAAGCTCTAATTGCTGGTTGGATGAAATGATCAAAAATTTGTCGGTCAGATGAATTATTGAGAAATAAAGGTAGTTCTAATACCGCAAAGTCAGCATAAGGATGAACTTCATCTTTATTTGGCTTAATTTGTACCTTTCATTGCTGTTCTCTTTGTTTTTTATTCTTTTGATCTTTGGATAAAGTGTAGTTCACAAAGTCATGTGCAATGAAAGCAGTTTTAGGAAGTACCGTTGGTTGTGCTGCTACAAAAGCTTGGGTAGAGTTGGGTAAGCCTAATTTGCTTGGTGCGACAAATTGTTTAACATCAGTGTATTTGCCAATTCTAAAATCAACGGTTCGCACAAAATTGATATCAAATTGGTTGTAAGGCGGATGATCTTCTCGGTTTTTTAAGGCCTGTATGACGTGTAAATTAGTAGCTAAGTAAACAGTAAATTTATTAGCTTTTTTATCTTTCTTGTCAACTTCCTTCCAGTCAATTAACCAACCCGTTCCGTGAATGGAACCAAAATCTATTTGACTTTTATCTTTCTCGGTTTTTTCAATTCTGTTAAAAAAACCTAAAGAAAAGGTTAAATCATAGTTGGCATGATCGATTTCTGCACTACCAGGAATATTGTTAATGTAATACCCTTCGAATTTATCAGGTTTTCGAAAGGCTGAACAAGCACTTAAAACAGTTAGTGCCAATAATCCACTAAGAAAACAAAAACGCTTGAGCGCAACTCACATAAACGAGTAATTCTAATAAAGGGTTAAGGAGCCCTGTTTGCAGTGATATCCCAAAGGATTAAACCACAAAATTTTTAAGACTTTTGGTTTTTAACAAAGGTAAATTGTTGGTCGTTGCTTTGCTTAATTTGCTCAAACAAATGGGTTTTATGTTGTTTAGCAAATTGAGCGTAGTACTTTTTAGTGTCTTTGTTACCAAAGATTAAGTCATAGGCCACTGTGTTTGTGCCATCGTTATTATGGCCATTGGCTTTTCAACGCAGCAACTGAGCTACCCCTCAAGCAGTAGTGGTGTTACTAGTAGACGCAAAATAAATGCCAGTAATTTGGTGCTTATCGTTTAACACTAAAGCACCACTAGCGCCACTTGTAAAACCAGCATTGTCCAAAACAAATCCCTTACCGTATTGTTGGTAATTACGACTTTGAAAAGTTTGTTCAAACCGAAATACCCTACTGTTGTCATAGTTTCAAACCCAGTTACTGCCATTAAAGTCATCACCTTGATTTCTAATTAGGGTTGGCTTTGCTATTGTCGATCGCACAATAAGGGGTTGTTCGTGGTTACTTTGTTGTACTGTTTGTCCATTTATTGTTGTTTCTGTAGATGTATCTTTCTTAATTTGATTAACACTCAATACATTAACCTTGTTTTCCAAGAAGGGATAACCCGATAAATAATAGCGGTGGTTTTTGAGTTCAGTTAATGTTGGCTTGGCAAACAGCTTTAAGCTATTACCTAATTGCTCATAAGTTTTGATGGCTGGTTGGATAAAATGGTCAAACACCTTTTTATCAACTGGGTTATCTAAAAATAAGGTTAGCTCTAACACCGCAAAGTCAGCATAGGCAAGACTGTCTGATGTATTTGCTGTATTAGATTCCTTTTTATCATCAGCATCCTTTTGTTGAATTCACTCAAACGGTTGGGTTTCCGCTTGCTCCTTTTGCTTATCGTTTTCTCCATCCTTTGGTTGCTTCTTTTTATTTTCTGTATCATTTTGCTTCTTTTTGTCTTTTGGCAAAGTGTAGTTGACAAAGTCATGCGCTGTAAAAGCGGTTTTGGGAATTACTGTGGTTTGGACATTAACAAAGGCTTGAGCATTATTGGGCAACTTAACTTGACTAGGTTCGACAAAATCACGAACGTGAGTGTATTTGCCTATTCGAAAAGCGGTCGTTCAATCGGCACTGGTATCAGAGTTTTTATAAGGATCGTAATCCTGTTGGTTTTTTAAAACGGCTGCGACATGTAAGTTGGTGGCTAAATAAACCCGAAAGGGTTCATTTAACCCCCCCCGCTACCTTTTCAATCAATGAGCCAGCCAGTGCCAGAAAAGGCGCTAAAGTTATCTTGCCCATTGTTAGTAGTTTTATTAGTAAAAGTTAAAGCAAAGGTTAAATCATAGTTGGCACGGTGAATCGAACCACTACTAGGAATATTACTTACATAGAACTGATCCCACTTTTCTAGTGGCACTGCAGCACAAGCACTTAATACAGTTAAGGAAGCTATGCTAGCGAGAAAACCAAGGCGCTTGAAAGCCACAATCATAATGGTGTTAATTCTAATAAAGAGTTAATTAGCTACTTTTACAACTAGCCACTAGTCGATTCATTACCCCAAGTTATGCCTTTTAGTCTTTTCGACAAAGCGGAATTCATCATTGTTAATTTGCTTAATTTGCTCGTATAAATGGGTTTTGTGTTCTTTGGCAAACTGAGCATAGTATTTTGTGGTATTTTTATTACCAAAGATTAAATCATAAGCCACACTTCTAGCTTCATCATTGTGATAAGTTTCTGGTTTTCAGCGTAATAACTGTGCTAAGCCTAATTCATTGTTAGCACCATCAACGCCAAAGTAAATGCCAGCGATTTGTTTCTTATCATTTAACAACAAAGAACCAGAAGATCCACCGCCTAAATCAAGATGACTAATGCCTATACTTTGGCCATAGATTCGGTACTTTTTGCCCGAAAACTCAAGATAAACCTTAAATTTCTGGGAGAGATCATAGTTCAAAGCTCAGGGACTACCAGTAAAATTATCGCCTTTATTCCTAATTAAAGTGGGTTTAACTTGATTAGTTGAGATAATAGCTGGTTTTTCATGGGATATTTGTGTATTTTCGCCTAAATATTCCTTTCTTTTTTCCCGTTTGGTTAAGAAATAGTGATGACACTTTATTTTTTAAAAACGGATAACCTAACACATAATAGCGATTTTGTTTTAATTGATCCAGTGTGGGGTTAGCAAAAATGTTAAGGCTATCACCTAACTGTTTATAAGTGCGTATGGCTGGTTGAATAAAGTGATCAAAGATTTTGCGATCTACTTTACTTTTCGAAAATAAGGGAATTTCTAAAATTGCAAACTCAGCAAAAGGCTGGACATCAGAATTCTTAGTATGACTGTTTTTCACCCATTGTTGTTTTCTCTTTTCTTTATCTTTTTGTTCTTGAGGAAAACTGTAATCCACAAAGTCCCTAGCAGCAAAAGCGGTTTTGGGAATTACACTTGTTTGCACATTGACAAGAGCTTGCGCAGCATTGGGTAAACCAAATTGATTGGGTGAAACAAAATCACTAACCTTAGTGTATTTACCTAACCTAAAGGTAGTAGTTCAACCAGCATCATCTTTATTTTTATAAGGTTCATAATCTTGCGGATTTATTAATGATGCTGCTACTTGTAAGTTAGTTGCTAAATAAGCACGAAAGGGATCATTCTCTTTCTTTTTATCAGGTTCCTTTCAATCAATTAATCAACCAGTACCATATGTGACACTAAATTTAGCTTCACCGTTGCTAGTGATTCTATTAGTAAAAGTAAGCGAGAAGTTTAAGTCATAGTTGAATCGGTGGATTTCCGTACTACTAGGGATATTGTTAATGTAAAACTCTTGCCACTTATCAAAGGCTGCACAAGCACTTAAAAAAGTTAAAGAGAATATGCTAACAATAAAACCAAGACGCTTGAAAACAACCAACATCGTGGCTTAATTCTAACAAAGGTTTAAGGAGTTAATTTTTAAGCAAAATCCCAAAGGTTTTAACTACAAATTTTTAACACTTTTGGTTTTTGACGAAGGTAAATTGTTGATCATTGCTTCTATCAATTTGTTCGTATAAATGGGTTTTATGTTCTTTAGCAAACTGAGCATAGTATTTTGTGGTATTTTTATTGCCAAATATTAAATCGTAAGCAACACTGTCTTTTTCTTCATCATTATTTTTCGCTTGTCAACGTAATAACTGTGAGAAGCCTAATTCGTCTTTAGGGCCATCAACACCAAAATAAATACCAACAATCTGCTTCCTATCATTTAACAGCAAAGAACCAGAAGAGCCACTGCTTAAATTAAGGTCACTAATGACTATACTTTTGCCATAGATTTGGTATTTCTTACCTAAAAATTCATGATAAAGCCCCGATTTCTTGCTGAGATCATAGTTTGAAGTTCAAGTGTTGCCAGTAAAATTAGCGCCTTTATTCCTAATTAAAGTAGGCTTAGCTGGATCGGTTAAAACAACAAATGGTTTTTCATGAAGTATTTGGGTAGTTTTGTCCATATGTTCCTTTCTTTGTTCCGTTTGATTTAAAAACAATGATGCCGCTTTATTTCTTAAAAAAGGATAACCCAACAAGTAATAACGGTTATGTTTAGGTTGATCTAGGGTTTGATTAGCAAAGATATTTAAGCTATCACCTAATTGTTTATAGGCTCTAATGGCAGGTTGGATAAAGTGATTAAAAATTTTGCGATCAGTAGAACTGTCAGGAAACAAAAGGATTTCTAAGACTGCAAAATCAGCATAGGGATGAACATCAGTATCCTTAGTTTGACTGTTCTTCATCCACTGCTGCTCCTTTTGTTCTTTACCTTTTTGTTCTTGGGGCAAGGTGTAATCCACAAAATCGTGCGCAGTGAAAGCCGTTTTGGGAATTGTGCTTGTCTGCGCTGATACAAAGGCTTGTGCACCATTAGGTAATCCTATTTCGTTTGGTGACACAAAGTCTTTCACGTTAGTATATTTGCCCAGTCTAAAATCCACCGTTCAACCCAATTCTGCATCAGGTTGACTGTAAGGTAAATAGTCTGCCTCATTCTTTAACCCTGCAGCTACATGAACATTAGTCGCTAAATAAACCCGGAATGGTTGGTATTGATTCCCCTCCTTTCAGTCAATCAACCAACCAGTACCATAAAAGGCAACGAAGTTTACTTCATTCTTCTTTTTAGTCTGAATTCTGTTGGTAAAACCTAAAGAGAAGGTCAAGTCATAGTTAATCCGGTGTAATTCAGCACTACTAGGAATATTGTTAATGTAAAACTCACCAGATTTATCAGATAGTGAAATTGTTGCACAAGCGTTGAGCACAGTTAATGTAGCTATGCTAGCTAGAAAACTAAACCGCTTTAAGGCAATTACCATGGTTGCTAATTCTAATAAAGAGTTAATCTGGCACTTTTGGGATTAAATGCCAGCTGATTAATTGCCTAAATTATTAGTTGTCTCTTGTGCTTCCACAAAGCGGAATTGTTCGTCTTTGCTTTCTTTAATCTTCTGATACAAATGAGTTTGATGATCTTTAGCAAACTTAGCGTAATAATTCTTGGTGTTCTTATCGCCAAAGATCAAGTCATAGCTACCCTTAGAAATACTTGAACCTTTATCGTTCTGTGGAGTACGTAAGATTTGGCTCATCCCAATGTCATTGCGTGCAAGACTACCAGATTGGTAAATTTCCAACACCCCAAAGTAAATGCTGTTCAATTTATTGTCTTTACCAATTAAAGGACTACCCGAAGAGCCACCCGGAAAATTAGTGTCATTCAACATTAAGCCATAGCCGTATTGTTGGAACCGCTTTTCGTGGTACTTCAAGGTAAAGTTGCTAAAGGAAGCAAAGTTCACCACACCTTTGTCTTGGATCACTACCCCATTAAGCCAGGACTCGTTTTGTTGGTTGGCAAAGAGTTGACTACCAGTGCTTTCATTGTAAGCACGGTAATTGTTAGTTAGGACGGGAATCCTGTCTAACTTATTAATATAGCTTTGATCTTTTGTAAATCACTGGCTCTTTTGGTTATGATCCGAATTGTTGTGATCAAAGCTCTTTTGTTGCGCTAATAAAAGGTTAGCGGTATTGTTTCCTTTATTAATCACTGGATAACCTAACAAGTAGTAGTTTTGTTCGATGAACTGGTTGAGTGGTTTGGCACTAAACAGTTCCAAAGTATCACCTAAAGCCTTGTAAGCCTTAATTGCTGGTTTGACAAAGTCATGAAGAATTTGCTGATCGCGTTGATTTTTAAGGTTTAGGTTAACTTCAATCACGGCAAAGTCAGCATAGTTGCCAGGAAGACTTGTTGTGATGTTGTTATAACTGTAACTATTCATATCAACAAAGTCAGTAGCAGTGTAAGCCGTTTTGGGAATATTGGAGGCCTGAATCGAAACCAAGGCTTTATCAGTTTGATTGGCTGATTGATTTTCTTCTTTAGAAATGTACTGCTTTAACTGCTCACCTTCTAAGTATTTACCCAAAGAAAAGAATTCAGTTTTAGTATTCTCAGTAAAATCTTGCTTGTTCTGATCTTTTTTGTAGGGTTCGTAATCCTGATCATTCCTTAAAGCATCAGCTACGTGTAAGTTAGTGGCTAAATAAACGGTGAATTGATCATCCTTTAGAAGGGAGTTATCAATGTTGCTTGTAATGCTAGGGACAAAACTGCCATCAATACCTAATGCCTTTTCATCACCCTTCCAGTCAAATAACCAGCCGGTGCCATAGATATCGTTAGACTTATTGGTAAAGTTCAACGAAAAAGTTAGATCATAGTTATGCTTAAAAACATCACTTTCACTAGGTACCTTATTGGTTTGGAAGTGAAAGCCACGGTTAACAGGCACTACCTCTGCGGTTGGTTTAGGTACATCAGTTGTAGGTGTGGTAGGTGTAATGGGACCACTTTCTTCAATGTTATTGGGTTCAAAGTTTGGCTGGGTTGCAGCACAGGCACTAAGAATAGTGCTGGTACCCAGCAGTGTTAAGAAACCCAATAACTTGAATTGCAGTCTCATAGACTAACTATTTTAATACAGATAGCCAAGGAGCTAAACCATTATAGCCCGGTTTATCTAGTAAATAAAGAGGCACTTAAACCATCAATGATTAATTGAATCAAGGATCTTTTTCGATATAACGGAACTGTTCATCAGTACTATTTTGAATTTCACTATACAGGTGGGTTTTATTTCGTTTAGCAAACAAAGCATAATAGTCTCTATTATTACTATCACCAAAGTATAAGGTCGTAACTGTATTTAACTAAACTTACCCCATTAACATCTTTAGGAACAGGCAATAGCTGGGCATAGCCAACACTATCTGATTCAGTAGCAGCTGAATAAATACTGCTAATCTTTTTGTCTTGGTTAAACACTAAACTGCCACTCGATCCACCTAGTAAATCAGTATCAGTTAGAGCTAAGCCATAGCCGTGGTTTTTATATTCATATCCATGATAATCAGTGATAGCACTTTTAAAAGTACCAAAATCAACAATTCCTTGATTTAAGGAATATTCATTAAGGAAGCCGTTACTGTAATATGTGTGCACTTTACTACCAGCAACTGGTTGACCTTGCCTTAAGTTTTTGGTAAGTGTTGGCATAGAACCATAAAAGTCATCATGGTTAAAACGTTGTTCTGTTTTGGTTCACTCAAACAACTTAGCATCATCAGTCCATAAAGTTGGATAACCCAAAAGGTAATGGGTATCGTTTTGGTATTGGGTTAATGGTTAACTGGCGAATATACCAGTACTGTCACCCAATGCTATATAAGCTTTCATCGCCGGATCAATAAAGTGATCTTTAATCTGCTTATCTTGCTTATTACTTAAATCTAGATTGATTCCAATACAGCAAAGTCAGCGGCAGGTAATCTATATTCCCCCAAATCATTAATAAAAAGTGTTGCCGTGTAAGCAGTTTTTGGCAATTTTGAAGTTTCAATCCGCACTAAGCTAGGTTCATGCATAGCATCAGGAACTATTTGCTTTATTTCATTACCATCAACGAACTTACCTAAAGTGAAAGATTCAGTTGGATCGTCAAAGTAATCAAATTGATTGAAAAACTCATAATCATTCACATTTCTTAAATTATCAGCTACATGTAAGTTAGTAGCTATGTAAGCAGTGAATTTATTGGGCTGATTATTATCCTTTCAATCAATGAGCCAACCAGTACCGTAAGTAGCTGTATATGGATCTTGAAACTGGAGCGTAAAACTTAGATCATAAAAGGGACGAAAGGGATCATCCTTTTTTGGAATGAAGTTAGTATTGAACTTTAAGTCAGAGGTGTATTCACCTAAAGCAGGGTCTGGTTTTAATGCTTGCTTGTCTTCTGGTTGAACTGCACTAACACTTTTGTTGTTTACAGGCACAGCCGGTGCACAAGCAGTAAAAACTCCTTGAACTAGTAAAAGTGTTAAAAAGACAAAACCTTTTAACCTATATCTAAGAAAAAGCGGGGTTGCTTTAGTAATAGGCAAATTAAAAAAACTTAAACAAATACGAAAAATTAATGAAAAAGCTAACTAATGGAGCAATGCTCGATTATATAAGCTTGCTTTAGTGTGGGTTGGTTAAGTTGGTCGATTTAACACAAAGCTAATGAAAGTATGCTTAGCTTTTTACCGGTGTTTAACTTAATTGAATTGTTTGTCTACCACATAACGGTACTGTTCATCAGTACTTTTAAGGATTTCACTGTACAAGTGAGTTTGCTGTTTTTTAGCAAACATCGCATAGTAACGCTTAGTGTTGCTATCACCAAAGATGAGGTCATAGCTTTGTGAAACTACACTTATACCGTTGACATCCCTAGGAGTTCGTAATAACTGAGCATAGCCAACACTATCTGATTCAGTAGCAGCTGAATAAATACTGCTAATCTTTTTGTCTTGGTTAAACACTAAACTGCCACTCGATCCACCTGGCAAGTTAGTGTCGGTTAAGGTTAAACCGTAACCCTGTTGACGGTAATCGTAATCGTGGTAATTAATGTTAGCACCCAAAAAGGTACTAAAACGCACAATACCTTGATCCATTGAGTCCTTATCGAGTAACTCATTGCTGTATTCGATGTGTGTCTTACTACCAGCGTTTGGATCGCCCTGCCTTAGGTTTTTGGTTAGTCTAGCCATATTACTGTAAAAATAACCATAGCTAAAGCGCTCTGCCCCTTGTTTGCATTCCCACAGTTGTGTATGATCAGTAGGTACAACCGGATAGCCCAAGAGGTAATGGGTATCGTTTTGATATTCAGTTAATGGTTTGGTGGCAAACAAACCGGTACTATCACCTAATTGTTTATAAGCCTTGATCGCTGGATCGATGAAATGATCTTTAATTTGCTGATCCTGCTGGTTGTTTAAATCCAGTTCTAACTCTAATACGGCAAAGTCAGCGGCAGGGATTTTGTAATCAAAATAATCCCAGTAATATTTTTGGTCGTTAAATAAATAAGGAAAACTATAATACTCGCTTTCAATAAAACTTGTAGCTGTGTAAGCTGTTTTGGGTAGCTGTGAAGTTGCAATACTGACCATGCTAGATTCACTAAAGGCATCTAGTGCCATCTGCTTAACTTCAATCGCATCGACAAACTTACCTAAAGCAAATGATTCGGTTGGTGCCTCTAGATCAAAATAACTGGGGCGGTATTTAAAAGGATCGTAATCATTAACGTTCCTTAAGCTGTTAGCCACATGTAAATTGGTCGCTAGGTAAGCGGTAAATTTGTCTTGGGAATTATGATCTTTTCAATCAATTAGTCAGCCCGTACCAAAAGCTAAACTTGCTTGGTCTTGAAACCTAAGCGTAAAACTTAAATCATAAAAGGGACGAAAAGGATCATCCGCTTTAGGAATAAAGTTAGTGTTGAACTTTAAGCCAGGGGTATATTTACCCGAAGCTGGATCTGGTTTTAATGCTTGCTTGTCTTTTGGTTGAACTGCACTAACAACTTTGTGATTGACAGAAGTAACCGGAGCACAAGCAGTAAAAGCAGTCTGTACCAGGATAATTGTTAAAAAGGAAATACCTTTTCACCTAAACTTGGAAAAGAGTGGAGTTATTTTCAGTTAGGACAAATTTCAAACAAATACTTAAAAATACACGAAATACCAATAAAACAGCGAAGCTAATTTAACAGTGCCCAATTATATAAGTTTGGCATAAATGTACTTAGGTTAAGCTGGAAAATTAACCCTAGCCCTCATTAAGTTGGCTTAGCTTTTAAATGGTGTTTGACTTGTTACAGTTTGATCGTTTTCGACAAAGCGGAACTGTTCATCGTTACTTTGAAGAATTTGATGGTACAAGTGGGTTTTGTGCTCCTTAGCAAATTGAGCATAGTATTTCTTGGTGTTGCTATCACCAAAGATTAAATCGTAGTTTTTGGACTTGTCTTTGTTTTGCGTAGCGCGGAGAATTTGCGATACACCGTAACTTTTACTTGGTAAAGCCGCAAAGTAAATACTGTTAATCTGTTTGTTTTGGTTAAATAACGGACTACCACTGGAACCACCCGGGAAGTCAGTGTCTTCTAACAATAAACCATAACCATGGTGTTGGTATTGCTTTTCGTGATAGTTAAAAACGTAATTGAAATACTTGCTAAAGTCCATTACGCCTTTAGGGTCATTTTGTGCAAAGGGTAAATAGTTAATTTGATTTTGTACCAAACGACTACCTGGAATTTCATCATCTTTCCGGATGTTACGCGTTATGCGCGAAACTTCCTTATTACTATTTTCATAGAGTGGTGAACTATAACCCTTCTGTGGTAAGTTTCAGTAAGACGTCTTTTCACGTTTAAGTAACGGATAACCCAATAGATAATGGGTATCATCAACATACTGATCTAATTGCAGGTTGGAAAAGAGACCCAAACTGTTACCTAGCTTTTCGTAAGCAGTTACCGCTGGATTTATAAAACTGTCAAAGATTTGCTGGTCACGATTGTTGGCTAAATTTAACTCTAATTCCAACACCGCAAAGTCAGCGTAACTTACGGCATTTTGGAATAAGTCCAACTCATAAACTGTGTTACCCACTATGTGATTGTATTTGTACTGAGCAGTTTTGATAAAGTCAGTGGCAGTGTAAGCAGTTTTTGGTAACTTCGATGTTTGAATTGAAACTAATGCATCACTATTCCGGTTATTGAGGTGCTTAAGATTTTGGTACTCTGGAGTTAACTTTTGCACAGTTTGGGCATCAATGTATTTACCCAAAGAAAATTCAGTGGTGATATCGCCAGGTAAAAACTCCTTCTGATTACCATCCTTGTTGTAAGGCTTGTAATCGTCTTTATTTCGTAAGCTATCAGCTACATGGAGGTTGGTAGCTAAGTAAGCAGTAAATTTGTTGTCCTGCTTAACATCCTTCCAGTCAATTAACCAACCTGTACCATAAGCTTCTTTTCAACTAGTACCAAACTTTAAGGCAAAGCTAAGATCATAGCCCGGCCGATACTTAGTATTTACTTTAGGAATAAAGTTAGTGTTGAACTTAAAACCAGGACTGTAAACACCCTTGGAGCTGTCAGGCTTAATTTGCACCTCGGCATTCTGCACAGGTGGTGTAGCAACAGCATCGGGCTCTATAACTGGTTCAGCCTCTGGTTTGGGGATAGTTTGGCTCAATGGTGGTTGAGGTTCAAAAATCACAAACTTATCACTTTCATTTTTGTGATTAGTGTTAGCTACTGTTAGCGTAGCAGAGCAAGCAGTTAGAAATGCTTGACTGGCGAACAGTGTGAGAAAACCAAAACCCTTGAGTTTAAACCCCATAGTGTGCTTTAATTTTAAAAAAGAAAGTTTGAAAGGTGAACCCTTAATTAAAGGTGAACTTAGTGCAGTACTAAAGTCGCACTTCTTCTAAAGGTCTGTTTTACTTTTTCGTTTTAGCACAATCACAGTCCATTTTGAGCTGAGTGTTACTAAAACGATTGGCAAAATAATCAGCCACAAATTTAACCATTGCTACAAAACTCAAGATAGCAAAGGCAAAGCCAATTAGTAAAAACACTGCTCCTAAGAAGTACAAGAAACCGGTGGATACTGGAGCATAGTTAAAGCAAACATAAGCAAAAATAAAACTAAGAAAAGTAATAAAGCTAAAAACAATCACCCAGATTAAGGTGCGCTTGGTGTTGTGCGGCGTTGTTGGCAACATGTTGCAAATTTCCGCTTTTAACTTCTTAAAAGAACACTTTTTAAACCACTGCTTTTTAGTGGTAGTGCTTTCTTTTTTGTTCATAGGTAATATTTAATGTCTTAGTTTCAATTTTAGTTGATGTTGTCAAACTTACCGCTAAAGCCTAAACACAAACTTACATAGCTTATTTAAATTTAAAGAAGGTTGTTGCTGTAGTTTTGAATAAAAATGCCAACCCGAATGATGGCCTTTTCATACTAAAAGAAGGAGCCTAAAAAAGAAGGATAATTCTGCAAACTGCAAAAGAGTTATAAGAATATCTTTAAACTAACTAGCAGAATTAGCCTTTGGTTTAAGCTTCTTAAAACTGACAAAGTAGAGAAAACTCGCTACCATACACACAAAACCAAAGGCTGCTAACACAAAGGTTAAAACAGAAAGAAAAGTTTTGACCGCAAATGGAAAATGCACAATCTCAAAGGGTTTCAAGTCAACTCCATTAGCATTGACATTAACCTGATACCCGTTAAAAAGTACTAATACACCAAGCGCTAAAGCTGCTATAAACAACAGTAAACCAACTGTTAAAAAAATAATGCTGTTGCATTTATTCCTATTTTTCCCTCTCATAAGCAATGATCTTACCGACACGTTTACTATGGCGCCCACCCTCAAACTGCGCCTGGAGAAATTCATCTACTATTTTAATGTTTTCTTCAGGAACCACAAAGCGGCTAGACAAACACAGCACATTGGCGTCATCATGTTGGCGTGCCAATGCTGCTGTTTTACTGTCTACCACTAAAGCAGCTAAGATACCCTTAGCCTTGTTAGCGGCCATACAAACACCCACACCAGTACCACAAATAAGGATGCCTAAACTATTAGGATTAGCTTGCATTGTTTGCGCTAACAAAAAGGCATAGTCCGGGTAGTCATCAAGTGGATCTAGCTCTGTAGGACCTAAATCAATCACTTGCAACTTTTGTTGTTCTAAGTGTTTTATCACTAAGCTTTTGAGCTCTAAACCAGTGTGGTCAGAAGCAATGTAAATTGGATGGTTCATTTGCATTAGTTTAAATCAAGGTGGTGTTGTTTAGCAGATGATTTAATTTTACCCAACAAACTAATTAAACCACTCAAAGTTTCCGAATAATTCTGCTGTTGGTAAAACTGGGTCAACGTTTCCAAGCTTTCGTTAATTTGGGGGCTTTCTTGGCGGTACTTATTGGCAAAGATAAAGGTATGTTTACACATTGATCTCAGGTTCATTAAACCTACTACATCAACAATCACATGGGTTAGTTGGTTTTGGAAGAACCTCACCTTTTCGTTTAAATAGCTAAAAGCACCCTCATAGTCATTTTTCGCTTGGGTAAAAATATCATCAAACTGGTGCTTAGCTTGACTAATAATCTTTAAAGTCTTTTCGGAAATAATTAACTCTTCATTCGCAATTAAGTTTTCAATTTGGAAAAACTTAAAACGAAAGTCATCTAAGGTATAGACAAAAAAGCGGTAATCATCAAACTTATTAGCTGACAGCGCAAACAGCTCGTTTAACAGATTTTTCAATCCATCCAATAACTTCATCGCTTCACTCATTAAGCGAAACAACTTCTCATAATCAATGAATTCGGTCAGCGCATTTTCCTGGTTGAGTAGTTGGGTTTTTTGTGCAATTAACAGCGAGTGTTCGTTAATCTGGTTAATGATGTTAACGGTATCCAAGTTGTTTTGAAACATTAACTCAATTTCCTTCATTAAAGTCTGTAACAAGGCGTTTTGTTGTTGGTAGTGGTTAATTGACTTTAAGAAGCTATCCACATTAGATACCACTAAGCTAATCGCTTTTTCTTTAACACTAACAGCATTAGATAACACATCAATCTGTTCCTTAGCTTGGTTAATGCGATGTTCACACAACGCTAAGTTAATGTTGCCCAACGCTTGGCTACATTCCGCCAAAGTAGCACCAATATTAGTAATTAATTCCTGAAACTTAGCTACCTCTTGGGAGTTAATTGCTTTGTTTTTCATCGAGCGCAATTGCGTTACCTTTTGACTCAAAATTTGGAAGTGGTAGTTCAAATAGCCTACTTTGACATCAAAGAAGTATTGTCTTGATGCGGTAATAATGGTTTGTGCTAAGTTATTGTGGAGTTTATTCAACGCTTGGTTTTGCTGTTTCACATCCTTATTTTTGATGTGTAGTTCCACATCCTTAAACAATAAGGAAATGCGGTTAATAATGTTGAGGTAAAACAAGGAATCGTACTTATGCACCAAAAAGTCATTAATAAAACTAACTAGTAAACAAAAGGCTTGTCGGTAGAATACTAGGGTATCATAACTACTGTCAAAGTACGCAATTGCGTTTTTAGTCAGCTTAACATAACGCTGTTGCTTGTCATGTAATCCTTGTAAGTTTTTGCGCAACAACTTAATATTACGGTTAAACCAAAAGCAGTTGTAAAGCGCTTTTGGTTCACTAAAAACTAACAGCTGTTCTAAAGCGGAATTGATTTCGCTGTTAAAGCTCTCTTGAAAACTTTTGAGCAATTTCACTGGTTGTTCCAGCGCTTTCTTTTCACTGCGCAACAGTTGAAACGGCACAATTACCGAGTTGAAATTACTGAGTTTTAACTTGTTGTACAGCGCCTTGGTCTGTTCCAAGTACTGCTGGTTTTTATGCACAATAATGAAAATCGTTAAATACCACAAGTAAAACAGAAAAGCTAAGACAGCTAACGATCCCAATAAAGCAGCAATGACAACCATTAGCGTTGCTTATTGGAAAGCTTTAACAGTTCAAACATTAAGTTTTGTTCAATCTTAATGTTGAGCTTTAAGTCCTTCGAGTGGTTCATTTGCCGCTCTAACTCGGCCTTACGCTTCAAAGTCGCTTCCAGATCTATTTCATCCTCAAAGACAAAGTTCTCGGTAAAGATCTTGGCCTCAGTTTTCTTAATGAGCACTAACCCCGCTCCTATGATGGCATTGCGCCGCGTGTTATTGGCAAACAGAATTTTGCAAAAGTGCGATTGTACGGCCGCAATTAAGGGCGCACGGTTATAGTTCAAACCCAAATAACCATTAGTGGTTTTGACTTGCGCACTAATAATGGCTTGATCGAGTTTAATACCACTGGGACTTAAGACTAAAAAGCGCAATGGTTGCATAGTTATTTCTCCTCAACAGTAGCTTTTTTAAGCCGCTTCGCTTTCTCCACTGCTTCCTGAATGGTACCAACAAAGAAGAAGGCCTGTTCGGGGAGATCATCATGCTTACCGTCCAAGATTTCCTTAAACGATTGGATCGTGTCACTTAAAGGGACATACTTCCCAGCAATCCCCGAGAACTTTTCCGCTACAAAGAACGGTTGGGAGAGGAAGTTGCGGATCCGGCGTGCCCGTTCCACAATGATCTTGTCCTCATCCGCTAACTCATCAATTCCCAAGATGGCAATAATGTCTTGTAGTTCCGCAAAGCGCTGTAAGATGTTTTGCACCCCTAACGCTACCTTATAGTGGTTAATCCCGACAATATTAGGATCCAATAAGCGACTGGTAGACTCTAAGGGATTGATCGCTGGAAAAATTCCCAACGCTGCTATGTTCCGGTCTAACACCGTTTTAGCATCCAGGTGGGTAAAGGTGGTAGCCGGAGCGGGGTCAGTCAAGTCATCAGCTGGCACATAGATCGCTTGCACCGAGGTAATCGAACCGGTTTTAGTGGAAGCAATCCGCTCTTGTAATTGCCCCATTTCAGTCGCCAACGTTGGTTGGTAACCCACGGCTGATGGCATCCGACCTAACAGTGCTGATACCTCACTACCAGCTTGGGTAAAACGGAAGATGTTGTCAATGAAGAGCAAGACATCCTGGTTGTCATGGTCACGGAAGTATTCGGCCATAGTAAGCGCCGTTAAAGCGACCCGCATCCGTGCTCCTGGGGGTTCGTTCATCTGACCAAACACTAAAGCAGTTTTGTCAATCACCCCACCCTGGATCATTTCATAGTAGAGATCATTACCTTCCCGGGTGCGTTCCCCCACACCAGCAAACACACTTAAACCAGAGTGTTCCTTAGCAATGTTGTGGATTAACTCTTGCACTAAAACCGTTTTACCAACACCAGCACCACCAAATAAACCAATCTTACCACCACGGGCATAGGGAGTGAGCAAGTCAATCACCTTAATCCCTGTTTCAAAGATTTCTAGCGTATTGGACTGGTCATCAAACGAAGGTGGTTTGCGGTGAATCGGGAGTTGTGGTTTTTTGGGTAACTCCTCCTTCCCGTCAATGGGTTTACCCAAAACATTAAACATCCGGCCCAAGACGTTTTTGCCAACTGGGACTTCAATCGGGTGGTTATAGTTAGTCACCTTTTCGTTGCGTGCTAACCCTTCAGTTGGACCCAAAGCAATGCAGCGCACAATGTCATCGCCTATTAGCTGCGCGGCTTCCAAAAACAGTTCTTCCCCAGCTAACTTAACACTCAAACAATCGTAAATGCGGGGTAACTGACTACTTTCCGTAAAGATGACATCAACTACCGGGCCAATCACTTGGTGGACCTTACCGTATGTAATGTTTTCCTTTTTCATTAACTAATACCTCCTATGATTTCAATAATTTCTTGGGTAATAGAGTTTTGTCTTAACTTATTAAATTGAATACTGTACTTTTCATACAGATCCTTGGCATTCTTAGCAGCGGCATCCATGGCGTTTTGTCTTGATGCTGACTCACACAACTTTGTCTCTACCAACCCACCGTATAACGCAATGTCAAAGAACTGGGGACTAATGAGCTTAATAATTGTCTCCTCATCGGGTTCAAAGTCGAGCTTTTGTTCCATTTTGACCGGGTTGAAGGCCTTAAACATCGCTACATCAAAGGGAAAAACCTGGAAGCTGTTGGCGTGTTGAATTAAGGGGTTTTTAAACTGGGTGTAAATGATGCAAATACGGTCTAATTGGTGTAAATCAAACTGCTCCATAATTTGGTCGGACACCTGTTGACAGTAATCAAAGCTCAAGTCACGATCTTGGATGTCAACATAACCAACGGTGGGGTTATCGGTGTCACCTTTATTTCAAAAAGACTGGTTTTTGCGCCCCAAAAAGAAGATTTTGTCACCGGGTTGGAACTTCGTTTGCAACAACTTGTTCATGTTAGTGTTGTGCTGTCCACAAAGACCCAACGATGAACTCATCATCACCCACAGGGTTTTGGGATTTTCCAGGGGCTTGGGCATCTTCTTTAAGCTGAGAACTACTTGCCCAACGGCCTTATAAAACTCATTGAAGAAGTAGTTAACTTCCTTAAACTGTTTTTTAAAGCGGACAAACTTAGCACGGGACACCATTTTCATTGCGTTAGTAATCTTAATGGTGGACTGCACCGTGGTCATTTTGCGCTTAATTTCCTGGATAAAGGCCATTATTCCAACTCCTCCATCGTTCCGTATTTAGTGATGTCGTAATCTGGTAAAGCACTAACAAAGCGTTTTACTAGTGCCTTAAAAGCAGTGTTAGTTTGTGCTTCCAACGCTTCACTAAAGCTCTTTTGGGTCGCTATTTCCTTGTAAATCGGGTGTTCCTTGTCAAATTCCTCCATAATGCGTTGCTTAAATTTAGCAATGTAATCCAGTGGAATAAACTTAATGAAGGACTTGGCAATCGCAAACAAGAATAACGCTTCATGGGTTTGTGAGTAAGGTTTACCGTTGGGTTGTTTAATCATCTCCATTACCCGCTTACCGCGTTCCAAGATTTGCTTGGTATTTTCGTCCAAGTCACTACCAAACTGACTAAAGCTGTCTAATTCACTGTACTGCGCTAGCTCTAACTTTAAGCTCCCGGTTTGTTGTTTAATTGCTTTAATTTGCGCTGCACTACCAACCCGCGACACCGATAACCCCACGTGGATGGCAGGGCGTTGACCGGAGTTAAACAGGTTTGACACCATGAACAGTTGACCATCGGTAATCGAAATGACGTTGGTCGGAATGTAAGCGGAAATGTCCCCAGCTTGCGTTTCAATAATAGGTAAAGCGGTAATCGAGCCACCACCATGCTCCTCGTTTAAACGGCAAGCACGTTCCAGTAAACGCGAGTGCAAGTAGAACACATCCCCAGGAAAGGCTTCCCGTCCTGGTGGTCGGCGTAACAGTAAGGATAAGGTACGGTAAGCAATCGCGTGCTTGGACAAATCATCAAACACAATTAGAACATCCTTACCCTGTTTGAGCCAGTACTCAGCAATGGTAATGCCAGTAAAGGGCGTGAGGTAGATCATCGAAGCGGGATTACTGGCTGTAGCACACACCACCGTGGTGTACTTCATCGAATCAGTTACCTCTAACTGGTGGACAATTTGCGCTACCGAGGAATTCTTTTGTCCCATCGCTACATAGACACAGTACACGTCCTTACCACGTTGGTTAATAATGGTGTCAATTGCAATTGAGGTCTTACCAGTTTGGCGGTCCCCCACAATTAACTCCCGTTGTCCCTTACCAATCGGAAACAACGCATCAATCGTCAAGATTCCAGTTTCCAGTGGTTGGTTAACGGTTTTGCGGGTCATTACCCCAGGGGCAATCTTCTCCACTTGGTCAAACTCTTCAGCCTTAATCGGACCACGACCATCCACCGGTTCTCCCAGGGCATTGACAATCCGGCCCAACATTACATCACCCACGGGAGTTTGCATCACGTTGTGGGTCCGTTTAGCCGTACTACCTTCACGGATTTTAGAGTAATCACCAAACAACGCCACCCCAACCGTGTTTTGCTCTAGGTTAAGCGCAATCCCCTGCACGTTGTTTTCAAACTCAATTAACTCGTTTAAGAGTGCGTTTTCCAAACCACTGACCTTAGCAATGCCATCAGCGACACTAATAACCTTGCCAATTTCACTGTTAAAGATCTGTTTGGAATACTTCTTAATCTCATTTTTAATGAGTGCTACATATTCATTTAATTTGTCTGCCATAATTAATTCCCTTTAAACTGTTCCATTAAACGTGATAGTTGATCACGGATATTCTTTTCAAATAACTGGGCGCTCGATTCAATTCGGATACCCGAAATCAAGCTCTTGTCAATTTCGGTTTTGTAAACAATCTTGGAATTGAGCTTCTTTTCCATTGCCGCTACGATTCGCTTGAGTTGGGGCGCACTCAGTTCAAAAGCGGTAATGATCCTAATGAACTGGACGTGTTTTTCATTCTCAACACAATCAAAGAAGTACTTTAAGGCTTGCTCGACTAGGTTAAAGTAGCCCTTGAGCACAATCGTTTTTAAAAAGTCAACAAAGAGGTCACAAAAGTGGTTTTTAAACAGCTTATCTATTAGCTTAAGCTTTTCCTTTTTGGTTAAAGTATAAGTATTGAGTAGAGCAGCTAGTGATCCATCTTTAAAGTTACGCGCTAGTTTTAAAAAGTCATGGCTCTGGTCATAGACCTTCTTAACCTTCTTTTCCTCTTCACTAAGTTGGAAGAGTGCCATTCCAAAAGCCTGCGCGTTAATCATGGTCGTCCTCTGCTTCTAGTTCGCGGATAAACTCTTCAATAAAGGCCTTGTCGGCTTTGACATCCACCTTTTTGATAATCAATTCCTTAGCAGCTAACATGGCTAGCTCGACACTTTCCTTTAAGGATTGTTCTTGGATCAAGCGCTTTTCCTTTTCAATTTCCTGACGCGCTTGGAAGATCATTAAGTTAGCTTGCCGGTTAGCCTTCTTTTCGATTTCACTTTTAAGTTGCAACGCCTCATAGTTGGCCTGGTCTACAATTTCCTTAGCAACTACCAGTGAGTTTTCATGGCGTTGGTTGGCTTGTTGGAGTAATGCTTGAGCTTGTTGTTCTAAGCTGTTGGCCTGGTTGACTTGCTCTTCCAAAAGCGCTTTGCGTTGGTTTAAGAACTTTTGTGTGGGTTTTCAAAAGAGGAACAACAACAAGAACAACAGGATCACAAAGGCCAACAAGTGGGCTAAAAAGACCCAGAGGTTCGGAAAAAGTTCGTTGATGACTGATTCACTTTTAATTTCCTTAACGTTTTCGGTACAGGAAACTAAAAACAAGGCAAACAGGGCAAAACTAAGCGCAACTAAGGTCGTTTTAAATACAAAAGTTGCTCGTAGTTTCATGATTAAGCGCCCGATACAAAGATAAGAATGAAGGCTATTAACAAGGAATAGATGGAACTCGATTCACTAATAGCAGAGCCAATAAAGATTAATTTAAAGACCTGCTTTTCCACTTCGGGGTTCCGCGCTACGGCTTCAACCGCTTTCCCAAAGATGTAACCTTGACCAAGACCCACGGTCGCACCACCGACCATGGTAATCCCAGCCCCAATGTAAGCCCCTAAACGTTCGGTACTTTTATTGGCAACATTGGTATTAGCTGTTGTTGTTTCGTGTAAGATGCGGCCAACTGTGGCGAGAATTTCATTAACGTGTTCCATATCTAATTAATTATTTCTTTTGCTACCTCCAAATTTTGCAAACGCTGCGCTGCTTCTTGACTTTCTTCCAAGCCAATGTTACGCATTTTTGCCCAATAATTATAAGTCAACATCACAAAGACATATCCCTGCAGTGTACCCACTACTACATCAAAATATACATGTAGGGCTGGGGTGAGAATTCCTGCAAAAATGGCACCTAAACTAAGCGCTAGTGGCTTATTGCTTAAAGTCGAAAAAGCAAAGAACCAGAAGTTATAAAAGAGCGCTAAAATCAAGGTTCCCCCAAGGATATTGCCCCACAAACGAAGCGAAATCGAGAACAGTGGCGCAAACTCACCCAAGAAGCTCAACGGATTGGGAATTAAAGTGGAGTACTTCTTGCCCTTAATCGTAATGTTGAAGGTAAAGCTTTTAAAGAAGGATCACCGTTGGTACCTAATCCCCATAATGACAATTCCCAAAAAGGTGGCTAGCCCTAAAGAAAAGGTAAAGGTCAGTGATGAAGTTGGTGGGGAAATGCCACCTAATAATCCAATCAAGTTACTGGAAACCAGGTACAACAGCAACATTAAAAAGTAAGGGGTAAACTTCTTGTTTTCCTCACCAATTAAATCCGCTGTAGTATCTTGGGCTCAAACAAAGAGCATTTGCATTAATAACACAAAGCTCGAATGGTTGCGTAACGGATCCGCTTTTCTTAACTTGATTCAGTAAACCAAGAATAAGGTGAGCAACACAATAAAGACCACAAAGATGCCAAAAACTTGATCAGTGGGTAGCAATGGTTTTCATCCCGCAACTGGGGAAATGTCAAAGATACCTTCGTGTGGTACGAGGTTAACTCCATTAGGTTCTTTTAAAACTATCGCCCGTGGTGACATGCTTCTTATTGAGCTTGGAAATCAGGTTTTTGCAATGATATTCCAAAGGTAGCCATGCAAATGATATCACTATTTTTAGCAATGGGAAAAATAACGTTGAAATTATTGTGGCAATCACATGAAAATATTGCGGTCTTATAAAAAAGGCGAGCAATACGGGAATCAGGTACAGCAAATACCGTGCCACAAAAAAGGAACCAAAGAAAACCGTAACTCGTTGTTTTGATAAATGCTTAAACTTTTCAAAAAAGTCCCCTAGTTTTAATAAACCAACCACGCTTAACGCTACAAACGGAAAGGGTAGTAACCAACCGTATAAAACTTTTAAGTGTTGGGTAGTTAAAGCTCAAACTAAAATACCCACTAAAACCACTAACCAAAGCACCAAGATAATGAGGGCAATTGCTTTAGTGAGTTTTTTATTGGTCCAGTTGTACAAGGCCGTTAAGCTTTTTGCGGTTTAATGTTTTTAAAGGTGTTTCAACCTAAGTACTTAGCATTATTGCCCAATTCCAGTTCAATTTGCAATAAGCGATTGTATTTGGCAATTCGTTCGGAGCGGGACATCGAACCAGTTTTAATTTGACCAGTGCAAGCCGCTACTGCTAGATCAGCAATTGTCGTGTCTTCGGTTTCCCCACTACGGTGGGAAATTACCTGACTCCAGTTAGCGTCCTTGGCAATGTTAATCGCTTTAATTGTTTCTGTAAGGGTACCAATTTGGTTGAGTTTAATTAAGATCGAGTTGGTAGCTTTGTGAGCAACACCTTTTTCAGCAATTGCTGGATTGGTGCAGTACAGATCATCACCAACTATTTGAATGTGTTGACCCAGTGTTTTAGTTAATGTTTCCATACCTTCCCAGTCGTGTTCGCTAAGACCATCTTCTATGGAAATAATAGGGTACTGCTCAGTAAGCTTCTTGAGGTAAGCGATCATTTGGGCAGTAGTTAAACTCCATTCCTTCTCGTCAAGAATTTTGGCCTTAATTCCTTTTTTGAAGACATAGCGCTTGGTGGTGTCATCGTAAAACTCACTCGCTGCTACATCCAGTGCAATCGCAATGTCACTACCTGGTTGGTACCCAGCTGCCTTAATTGCTTCGACCATTAAATCAAGCGCTTCTTCCGCTAACTTTAAGTTAGGCGCAAAACCACCTTCATCACCCTTATTGGTGTTTAAACCCCGTTGCTTTAACAGCTTTTGTAAAGCATGGAAGGTTTCACTCGCCATCTTCAATGCTTGGTGCATCGAGTTAGCACCCAATGGCATAATCATGAACTCCTGGAAGTCAATGTTATTGTCAGCATGTGCTCCACCGTTAATGACGTTTAGCATTGGTACGGTGAGGATAAACTCGGTTTTATTTAGACCCATGACCTGGTTAGCAAGGTACTTAAACAAGGAAGTCTTTTGGGCGCTAGCAGCTGCTTTAGCCACAGCTAGAGAAACTGCCAAAATGGCATTAGCACCTAACTTAGCCTTATTGGGGGTACCATCCAGTTGGATCATTAAAGCATCAATGGCTGCTTGATCAGTCGCATTTAAACCAATTAGTTTAGGGGCAATAGTTTGGTTAACGTTTTGCACAGCTTGGCTCACCCCTTTGCCAAAGTAAGCTTTGGGATCACCATCGCGTAGTTCAATCGCTTCTTTTTCCCCAGTAGAAGCCCCAGATGGAACCATCGCTTCTCCAGTATGACCACTAGCTAACTTCACAACACAAGCAACAGTGGGAAAACCCCGTGAATCAAAAACTTGGTAAGCAAAAAGATCAGCAATCTTGAAAAGATCGGTTCCAGTTTGTGCACTCATATGACGCTTCTAACTAATATTTACTTAAATTATATTGACCAATAAATTTAGGAAATGAAACAAATCTATTTTGGTGGTGGCTGTTTTTGGGGTACCCAAAAGTATTTTGATCTGATTAAGGGTGTCCAAAAGACCAGTGTGGGCTACCTCAATTCCAACATGAAAAATCCCACTTACGAACAAGTATGTAGTGGGCAGACCAACGCAGTGGAAGCAGTGTTCGTTGAATATGATGAAAACAAGGTAAGCTTAAACGAACTTATTGATGCTTTCTTTAAGGTCATTGATCCCACCATTAGAAACCGTCAGGGTAATGATATTGGTACCCAGTACCGTACCGGAGTTTATTGGGTAGATCCCCAAGACGAGCAGTTAATTACGCAAAAGTTTAGGGAACTACAGGCCAATTATCCAAAACCAATTGTGACGGAAAACCGTGCAATGGAAAACTATTTTTTAGCAGAAGAGTACCACCAGGATTACTTAAAGAAAAATCCTCATGGTTACTGTCACATTAAGTTTGACTAGTTAAAGACTTCTGGGTTTTTCACAAACAGGAATGCCTTAATGATGTTGTAGCAATAGTCATTAATCCGTTCAATGTGTTTCACCGACAGAATTAAGTCAATTAAGAAGATGCGATCACTCAGCTTCTCGTCTTTAACCTTGTCATAGTATTCAGTTAACACGACGCGGTACTGCTGTTCAAACTCTGTCAGAATTTTAGTGACATTCTCAAACTGAATAGTAAGCGGTTTTTCCTTATCTTGTAAACTGGCAAACGTTTGCTTTAAGTTGTTCAACACACTTTTGTGCAAATTAACTAACTTACTAAAGATGCTGACATCCAAGTGCTGGTAGTGCTTGACAAACTTAGTGAGGTTATTGGCATAATCACAAATGCGTTCCAAATCACGACTACACATAATGATCGTAATTGTTAAGCGTAAGTGGTTAGTTAACGGGGAGTTTTTTGAAATCGCTCAAATGGATTCGTTAATTAGTTTAAACTCCGATTTATTGGAAAAGTCTTCCATTTCGTAAATTGCTTGAATCAACTTTTCGCGCTTAGTTACATCCGCTTCACATAACAGTTTATTGAGTGTTTCATGCATCTTAATGACGTGCTGAAAGTAATCAAAAAAGAGTCCTAACAGCTTTTTTTCTGAACGCTTTAAAATTTGGTAGTTAATGCTTTCCATCGTTAATTCCTCCCACTAATGTAACGGTTAGTTTCCTTTTGCTTTGGTCTCGTAAAGATTTGCTTGGTAGTACCTTGTTCCACTACCCTTCCGTTAGCAAAGAAAATCGTTTCATCAGTGATTCTAATAGTTTGTGCCATTGAGTGAGTCACAATAATAATCGTGTATTTTTCCTTTAATTGCTGGATTAACAGCTCAATCGAGTTAGTGGCAATTGAGTCCAACGCACTAGTTGGTTCATCCATTAATAAGACATCGGGTTGTAGAGCAATGGCTCTGGCAATACACAACCGTTGTTGTTGACCACCCGAAAGGGTATTGGCATTACGGTGTAAGTTATCTTTGACCTCGTCCCATAAGGCGGTTGACTTTAAGGCCTGTTCCACAATTTCATGAATCGCTTGCTTGTTATGAATGCCATGGGCTCTCAGTCCATAGGCAATGTTTTCAAAGATGGAAAAGTTAAAAGGGGTGAGTTGCTGGAACACCATACCAACCCGTGTGCGCAAGGTTAAATCATTAATAATTCCGGAATTAATGTTTTTACCCAAAAAGAAAATCTTGCCATTTCAGCGCACATTTTCATTGAGGTCATTTAATTTATTTAAACAACGGATAAAGGTGGACTTACCACAACCGGATTTACCAATTAAAGCGGTAATCTTATTGCGCTTAATTTTGAGGTTAATGTCAAATAAGACCTGCTTGTCCTTGTTGTACCAGAAGTTAAGGTTTTCAATTTCAAAGACATTCTTCTTGTCAAAGTCTTGTTTGACAACATCGTCCTTGGTATTCTTAATTTTGATGAGGCGTTGCAACACATCAATTTCACTCTGCAAGTTAGCAATTTCCTCAGCTTTGGTAGTGGGATCTAACTTAGCAACTTTTTGCTGGTATTGCTTAATTTGTTTTTCATATAAAGCACGGTACTGCTTCACCTTTTCGCGCTTTTGGATGAAGTTGTGTCAAATTGTTTTTAGGCCTTTTTTCATAATTGCAATTTGCTTTTAATTTGTAATCACTTTTGTTTATTAAACGAAAACAGGTAAGGAATTAAGTAAGAGCTTAAAAAGATTAAGAACATCAGGAAGACAATTGATACCAAAGCGGTTTCCAGCATTACATCAACCGCCGTACTGTTAGTGGAAAATAACTGACCATAAATTCTTGTGGTTAAGGTTTGACCCGGTAGACTTAAATCAAACAAGTTGCTGGATGCTAACCCAGCGGTAATAAAGAAAGGAGCTGTTTCCGCAATAATTCTGTTAATCGTTAAAATAAGCGCAATAATTAAACCCTTCAACGCCCCTGGTAACACAATTTTCCAAATTACTTCCCTTTTACTTACACCTAAGGCATAAGCACTTACCCGCAAGTCCCAACTGACATTATTGAGTGCTTCCTGACATGTGCGAATTAAAAAGGGTAAGACCACTACACTAATAGTAAGAATACCCGCCATTAAACTAGTACCATTCGCACCACCCGCACTTAATTGCAGTGTTCGCAAAAAGAAGGATAGACCAAACAATCCGTAAATAATCGAGGGCATCGAGCTGAGCGAATCAATTACAAAACTAAAGGTATTTTTTGCAATCCGCGACTTCGTGTATTCGTTTAATCAAATGGCAATTAACAAAGCGATTGGAAAGGTAATACCAATGGCAACCAGAATAATCACCAAGGTATTGACTAAAGCTCGCCCTGTAGTATCTACACCAAACGAAAAGACAGTGGAACCACTGCGCTGAATCGCTTGACTACCATTAATAAAAACAAACAAGAGAATCCCTAACACAAAACCAAACGCTAAGACGGCACAAAAAATTTCCTGCAGGATTTTTCACGATGTGTAAACCTTATTTAAGTGGTTAGTCTGCATCCTTTCCTTGAAGAAGGTAGCGCGCTGCTCAACATTAGCAGCTGTAATCTTTTTAACCTGTTGTCTTCTAGCAGTTAAATCAGTAAAAAGAGCGCCAATGTTATTAGGAATAAACCACACTACTTGATAAATAAAGTTACTAATCTTCTTTAGGAAAGGATAGCGTTCCAGCGTTTTTGGCCGGGTCGATCAAATGGCAAAAAAGTTCAAGAAGGAAATTAAGACAAAGATAATAATGCCAAAAGTGTATAAGACCCCACTAACCTGTTCATCGCCGTTTTCCGAAAAGATAAAGGTGGAGATAACAGAACCCAAAGTTTTGAGATCTGAAGTAAAGAAACGATCATTAGCAATCACTTCCTGGTAGTTAACACTCTGCAAAATAAAGTTCACAGCCATCGTTTCGCTAATGGCACGACCAAACGCTAATACCAAGATTACTACTAGTTGGGCTTTGATTTCCTTTTTAATTACCTTATAAATCACACTGGTTTTGTTTTCACCAAGTGAAATGGCAACCGTCATTAAGCTAGATTCCACGTGCAGTAAGGCATTGGTGGTTAAGGAAATCACAATCGGAATGATCATGAAGGACAGCATCACAATCACGTTAAGCAGTGATAAGGGTGGCAAGTGCAACACGTCACGGAAGATACTGCTGAGAATTTGGGTAGCAAACAATCCAAAGATCACTGAAGGGATCCCGGACAGAATGTCAATTACTAAAAGTAGTTTTTTGCGAATCCGGGGTTTGCAACGATAAACCAAGAAAATCGAAGTGCGAACACCGATATAGCTCGCAATAATTAACGAGCCAATCGAAACCACAAAACTAACTAACAGCGGAAACCAGATGCTCGCTTTTTTAGCACCCAAGTTAAATTCCAAACCCAGTAAGGACTTGGTAAAGTCAGGACCAGTTTTAGCTGCTTCAATTAAGAGGAAAATAAAGAAGCTAATGAAGAGCAGTAAAAAACAAACAGCCACTGTTTGCGATAAGTACCGCAGCCAGTTGTCTTTTTTTAAACGGCTTTTAATTTTCTGTTTCACTAGAGTGCACCGAAGGTTTGGTTGTTCAGACTGTAATCATCAACCCAGAAGTTGGCAATGTTGCTCATTTCTGCATTACCTGTTTTAAACATCTTTTTCTTTTCGTCAGCCGTTAACTCTAACACACCAAAGTCATCGTATATTTTCTTAATTTCACTGTTGTTGCTAAATAACAATCAGTTAAAAAAGCGTTGGATACTTGGCAGTGTTTTTTGTTTTGGTAACAGGGAAACCACTGAGTTCAACGGGCGTACCCATTTGTAAGTGTTGCTAGAAACTGCTTGACTGGAGGGGGTAACTGCGTTATTATCATATTTGACTTTTAAAACCTCAAAACCTTCACTCTTAATTAGGTTCATGTTGTTATTCACAAACCCTAAACTGAGGTACACCATGCCGTATAAATTGGGTTGTCGCAAAGTTGTGACAAAGGTATTAAAGGCCTCAATGTTGGTTTCACTAGTTGGTCGCGCATTGGGGCCATAGTTGCGTTGACCTTCCAAAATTTCTTTGGAATCTTTATCTAAAGTTTTTGTCTGTGTAAGACCCGAAAACTTCAAGAAGGCTTCAGCGGTACCAGAAGCAAAAGCGCCCCCAGTACGGGGGAAGCCTATCAAATTATGATTACTGTTTTTAGTGGTTTGTCCGTTTTCGACAAACTTATTAATGTCAACGCTTTTGCTACCAGCGAACAGATCGTAAAGATCGTTGAGGTTGTCTTTAGTGAGAACTAGTTTGCCCTTAAACTCTGATGGTGCTTTATAAATAACAGCAATGGCATCTTTGCCAAGTGTAATTGTTTTGAGCTTTTTGTCCATCCACAACTGCTTGTTTTCCTCAGCATAGCTCTTGGTGTTTTTCGAGACATTACCAATGTCAGCTAGTCCCTTGGTGATTGCTTTCACCCCAGCACTAGAGCCACCCGCTTGGACACTAATTTCTACAAGGTTATCCTTGTCATTGTGGTTCAAGACATAGTGCGAACTGAGTTTGCTTAACAACGGTTGTACCGAAGAAGAACCAACAGCACTAATTAAATTAATATTGGCACAACCGCTAAAAATAACAGCGGTTAGTGATAGTAATGCTAGTCTAGAAAGTCTTGCAACTCTTAGCACTTTTCTAATTGTGTGCGTTAATTTTAGAAAAACTGGCATTTTCGGTACTTTTTAACCAACTTTTAATTAAGCAAGTTGCATTTCTTAGTTGTGCTTTAAGTAGTTAAAAACAACGATTTTCTGGCGTTTATTCTTTGCCAATTGTTTCCTTTGCTAATTTCTTTTTACGAGAGGCTGCTTTTCTTTTAATCATTCAACCAATGGCCGTGGCACCTAAAGCTAACACTCCAGCAATCGCTGATAAAGATCAGGTCAACATCTGGTTAGTTTTAGCACTAGTTTAATCAGTGTCATCTTTATTGTCCAACTTTTCCAGTGCAAAGGCTTTGTTTTGGGTGTGGAAATCAAAGACTGGTTTTTGTCTAAACTGGAAGTTGTTTTCCTTTAAGAATCTAGCTAGTTGTTGTGCTAGCTTAGTTTGACTGTCATTTTTAATGTTGAACCAAACTGACTTTTCTAGACCATCTTTATTAATGAGATATAGCATTTGCTTATTTTGTGATAGCTTTAAGTCATAGTTAATTGCAAAAAGTCTTTGACTACCCAACATTGCTTGAATGGAGAGCTGACTAGTATAGCGATCAACATGGAACTCTAAGTTGAACTTTTGGTTGTTTTTTAAAACGGTTAAATAAGCCATTTTATTTACCGGATTCAACCTAGCATTCTTCGTTTCCACATGGAGGTTCATTTGACTCAAAACAATGTTGAGTTTTTCCAATAACACTTGTTCATCTTGCAAACTAAAGTCTGATTGAAACTCAAACTCATAGTCATCTATTAATTGGATGTTGGATGTTTCTATATCTAATTTGCCAATTTCGAATTCATCAAACATAAAGCTCACTGTTTGGGTTTCCAAGTGGGGTTTAAACCACAACTTTTGTTTGTCAAGCAACAGCTTGCGCGTATTAATTTTGAACAAGTAAGTGCGTGTGTTGGGCTTTATTTGATTTTGTTTAACAAAGTACATTTGCCCTTTTATAATTTCGGGATCGATCGTGGAACCTAACTTAAACAGTTCGTTGCTGTTGTGGATGTCGGCTAACACATAGAAGAAACCTTTGTTTTCGTTCATGTTTCCTAAATCAAGTTTGTATTGTCGGGGATCTTCCACAAAGGTTAAGCTGTTAGCATCTACATCTAACACGGTTTTATCAACACCAGCAAAACTGTTTTTAATGACAAAAACAATTTGTTGCTTTTCAAAATCATTGAGTTTTTCCATGCGTTGCTTTAACTCACCTACACTAAAGCTTTTAATCGCTTTGTGTAACGGAACACTAAAGTCCTTTTATTTAAATTGCTGGTTGCTAAAGTTACTGCGATATTTAAATTTGTACTGGTAACCTTCTGGAATTTTGTTGATCGTTGGTAGGTTTTTCACTGCACCAAAAAGTTGATCAACTACTTGGAAGTGCAAGTTTTCTGATTCATAACCGTCAACAATAAAATCCAAACTTAAAAAGTTAATGGTGTATTCGTTGGAAGCGTGTTGTTGTGGTGTCAAGTATTTTTCTCAAGTTTTGATATCAAACACATTGACATTGTTTTTAAGCAACGTAATTTCTTGCAAGGTATCACCAATTTTGACTTTAGCTAAAAGTTTGGCTCGTGCAAAAATAGTTTGCAACATCTCTGGTAAGTTCCTAATGAAGATAAGGTGTTCTTTATTGGTAGGCTCCATCCCTAATAAGTCATAGAGGTACTGCAAGCTAGCAAAATTAACGTTGCTGTCCTTGGACTTTAAAGAGTCAAACTTTTGTAGCGCTGTTGCTTTATCAGTAGCTAAATCAATCTCTTCGTTCAACTTACTAATTTGGGCAATCGCTTGAGTAAACTCCTTTTTGAACATTATTTGTAATTGATCTTTTTGAGATAGATCTTTTTCATTAAATGTTTTGGACAGAGTATCCACTACCGAATTAGGGTCAAATTGTAATTGTGAAAGTCACAACCTAGAAAGTTGACTAAAACTAATAGGTGGTTTTAGATCTGCATTATGTTGTTCCACATAAAAGTCAACACCAAAGCGCTGAGCGTTTACTGGGATAATTGGGGTGTATTTGAAAATGTCCCACTTATTGCTAAACAAACTTTGTTCCTCATCATCAGTTAATGGCTTTACTACAACGTTTTTACCCATATTCAAGATTGATTGGGTTACTTGGCGGTTTCTAGTAAAGGCTTCCATGATCTTACGATCTTTAATTACCTTATCTTTAAAACCATTGGAACTACTTCAATATGCATTTGCTTGCTTGTTATCAATGGAAAATACTCAAGTTTCTTTTGGTAATACTGGCTCATTCCACTGAACATTATCTTGTTCAAATACTCTAGGTGTTTTAATTAACTCCACCTCTCAAAAAACACGATAAATGTAAATGGGAGTTGATTCCAACCACCAGTTATTTCAAAGAGTGGCAATGAAAATATTGTTTGGTCTATCTTCAAAGAAACCATCATTTTGTGTTATTGCATAGGCATTAAAAATAATAAAGTAATTGCCATTTTTTCCTTGTTTAATTCACTTAGCATTTTGAACAATGTCATTTATGTTAGGTGACTTAACTGATAAGGAACGGTTCATAAAACCGAAAAAAACATTAATCCATTTAAAGTCTTTATTTCTATTCCATACAGGACTAAATAGCCTTTGGGTTTCTAAGTTATAAGGAATAGATCATCCTGCGTTTTTAGCTTCTTCGGCAGTTCGACCAGCGCTTAATGTCACATATTTATCATGCTGTGGATACCACGGGTCATTAATGCGAGATGGTCCAAAACCAGACTTACTATCATTGTGAAAGTGGAAATACTGAAGGTCGTGTATATCTGATTTAAAGGAGTTTCTAACTCCGGGATAGCTAAAAGTTTTGTTCCATGCCAAACTGCTAGTATAGCTACTTTCAATGCCTAAGTTAACATCTTTGATGTAATAGATATTGTTATACCAATTAAAGTTATTTTCTCTAAGCAATTGGTTATACCGGTCATAATAGCCATCCGCTAGCTGAGTTCAAAGGGTGGAATAGTTTCAACCAAAAGTAGTTTTGTTATTTGGTTTATAAATTAAAGGATTTTCGAAATTAAAGTCTTTACGGAAATAACTATCCTTTAATTTGATCCCAACAGTAAACCCTTGATTGTGGTGTGCTGAGTTAGAAATATACAAACTACTATCAACAAGATTTTTATCCAACGACGCGTTCGCTAAAAGAGTTGGGAACGCAACAAAACCTAATAAAGGTAAATAATGATATGAGCGCATAATTAATTCACTTTTTCATCTATTAAATTGCTCAAAAAGGGAATTGACGCCAATTTTTTAAAAGTTTTTATTTATCCGTTTTGACAACTGCTTTTTGTTATTTTTTAAAAGTTTTTATTTATCCGTTTTGACAACTGCTTTTTGTTTGTGCGATTTCAAACTCTTTTTGCGCTTGTAAAAGTAAAAACTAAAAGCAACTACCACTAAAAACAGACTACTAGCAGCAATTACTAAGTAACCAATAAAATTGGTGTTTTTATTTTCACCATTATTTGGTTGAGCCTGTTTGGCGATTTTAAAAGTTGCGGTATTATCCTGCAGTTTAAGCGGAATCTTTTCTTTTAAGTACATCTGCTTACTTTGAAGAAATAACTGCAAGGTATCGAGCAATTTAGCTTGGTATCGTTGATCAGCAACAGACCAGATATACATGCCTAAATGCTTGTTGCGGTTAACAAGTTGTAGAGTGTTTGAATTTTTAATCACTAGTTCATAATCCAACTTAAATAATGGTTGTTCGTCAACCTTAACAATTACATTAAGTTCACTAGCTTTTGGATTAAATTCAACATCAAAATCAAACGATTGATCTATTGACTTTGTAGTGAGTCTTATTTGTTTGGGAAAAGTTTCTATGTTAGTAGCTTGTTCATCAACCATTAAGTTCATGCCAACTAATGCAAGGTTCAGTCTTCTTATTAAAAGTTGCTTATCAGCTAACGTTAAGGTAGAAACCAAATCTACTTGGTAATCGTTAAAGAAAGCAACGTTAGGCAATTTGGGATCAAATTGATCTAATTCAATCCCGTCTACAATTACCTTAACTTTGTCATTGTTAGCTTGAAATCAAACGTCATTGGCATTCAAAGCAATTTTGTGTGTTCTCATTTGAAACAAGTAAGTACGAATTGTTGGAATCTTTGTTTCTTTTTTGAGAAAGTACATCTGGCCTTTAATAATTTCCCGATTGGAATCTGTGCCTCATTTAAAGAAATTATTGCTGTTAGTAATTTCTGCTGCAATATAGAAGATGCCCGTTTTAGGATTTGTCGGAATTAAACCAGGAATTAGTTCTTCAGGATTTTTAACTAATTTGATTGAATTTAAGGCAACAGTTAATGCCGTATCATCCTGAAATGAAAAAACCATTTTTAATTACAAAAGCTATTTGGTTTTTCTGCTCTTCAGTCAATTCTTTATCTTGGATGTCTAAAACGCTAAACTCCTTGCCGTTATTAACGACGGGAATGCTAAAACGATATTTATTGAAATACTGTTCGTTGTAATTTGAGCGAAACCTTAAACGGTATTGAATGCCCTGGTTTGTTTTTTGAATGGTTGGTTTATTTTTAGCAGATTCAAATAAAGAAAACACAGGTAAATTCTTCGCTGTTCAACTAGGATATTGATCTGATATGAAATCAAAAGAGAGAAATTCAACTTCAAATAAATTAATGCTAATTTGATCTAAATTTAAGAATTGTTCAAACTGGCCTAGATTAAAAACGTTATTGTTGTTTTTAACTAAAGTAATCTTTCTTAATTGCCCACCCATCCTAACTTGAAAGTTAATGGATGCAGACTCAAAAATTGTTTTTAAAAGCCTTGGTAAATCACGAACAAGACTTAATTCTTGAGGATCCCTAAAATCAAAATTTAGCAGTTTATAAAGGTAGCTTAGGCGTTCTCATTGCTGTTTTCGTTCAACATTTTTTAAGCGTTAAGAAATTTTGTAAAACTGTATTTTTATCAATTGCTTGATCAACTAACTGATTAAGCTCTGTAATTTCTTGAATCGCTAAGGTAAATTCCCTTTTAAAACGTAATTTCAAAAGAGCTTTTTGGCCAAGCGTTCGGTTATTAAAGGTGATAGAAGTATTATCAACTATTGAATCAGGGTCAAACTCTAATTGAACTAAAATAATTCGCGATAGCTGATTGAACTGAATAGGTGATTGATTTTCGCTCTCTACTAAATTTTTTGTTAAGTAAAACGAGCTGTCAATTACATGTGAATTGATAGTTGCGCTAGAGAGCAACAGTGGGGTTAATGAAAAACCCATTAATTTTCAATATTTCAAAAAAGGCATGGCAATCTTTACATTCAATAAATTGAACTAAAGTTTTTAACTTGCCAATTTTTAGAAAATTAGTGCTTTAAAGCTTTTTAAATTACTAAGCTAATTGTTTGGAAAAGTGAGCAATCTTGCTAAAGATTAAATGGTAGTAATAATCCAATTGCTTTTTGCGCATTTCGATTTCAGCAGGAATACCTTCTACTAAATCATTGCAAAGTTTTTCAAAGGCAAAGAGAATATCAGCAATCTTTTCTTGAATTTCTAGAGGTGGAAAAGAGAGTTCAATTTCAGCCATTACTTTTTGGCTTAGTTTTGGTCTTGAAGCTAGATTATGAACGAATTTTGGTGCTTCTATCTTTAATAAAAAGGATAGGAATTTGGTGCAGATTTTTTTATTTTTAACTTTTAAAACACCGCAATCTTGTGATGCATTAAACTTGCCATTACGATAAAAAACTACTCCAGCATAACCATTTGTAGTTCAAGTGATGTATTCACCATCAAAGTCGCAATCTTTAATGCGTCCTAACTCACCATCATTTGTGGTAGCTGCGGAATAAACTGGATTTTCTCCAGGATTATTCCTAATATACGCTTTAGTGATCGCTCGACCTCTTCTAATTTCACAAATATCCTTTACTTGAAAAGTTTCGAAAGGTATATTTGCTCCGTAAATTTTTCGGATATTTTCTTGGTTGAGCAGATAATCGCGATAGAAGGCATACTGCTTCTTTCGCTCTCTTAGCTCGGCGCTTAGCTCGGAAAAGTATCTAAGATAGTAGCAATTTTCTCTTGAATTTGCAAAGGTGGGAAATCTAGCTCAATTTCTGCTAAAATTCCTTGCATTAATTTAGGAATAGGGCAAGCATAATTAACAAATTTAGGTGTTTCTAATCTAAGTGCATAAGCTAAAAACTTAGTATTAATTTCATCACTTTTTACCTTAAGAATTCCACAAACATTGGTTGCGTTAAATTGACCGTTACGATAAAAAACACTACCAGCTTGGGCTCCATCAGTTGTTCAAGTGACGTACTCTCCATTAAAATCACAATCCTTTATCTTACCGATTTCACCGTTATTAACTGTTGCTGATGAATAAACAGGGAATTCTCCTGGATTGTTTTTGATATATAGTTTGCTGATAACTCTACCGCGTTTAATATCGCAAATATCTTTGATTTTGAAACGTTTAATCAACACAGTAAAACTTAATCTCCTTTAAGTTTAAAGATTAAGTGGGGAACTGGACAGCTCAAAGTTTATTGAAAAAAACTAACGCTTGGTAAATTGTGGAGCACGGCGGGCACCGTATAAACCGAACTTCTTCCGTTCTTTAACCCGCTTGTCACGGGTAGTTAGTTTCTTGGACTTGAGAATCTTTTTCAATTCTGGATTGAACTGTAAAAGCGCGCGAACAATTCCTAAACGAATTGCACCAGCTTGTCCGGTAAAACCACCACCTTTAACTACCACATTGATATCAAAGTTCTTTTTAAGATCAGTGAGATCTAAAGGTTGTTCCATGTCTTGGATCACTATTTTATTGGGAAAGTATTCACTAGGATCACGACGGTTAACAGTAATCTTACCCTTGTCTTGGGTAGGCGTTAGATAAACTTTAGCACTTGAAGACTTACGGCGTCCAAGTCCATAATAAGATTGTTTTTCCATTTATATTAGCTTCAATTTAAAGTGGTAGGTTTTTGCGCTTCCATGTTGTGTTTATCACCTTTAAACACGTGTACCTTGGTGATTCAACGACGGCTTAAACGGTTATCAGGTAACATGCCCTTAACAGCATTATAGACCAGTTTGTCGGATTGTTTTTTCAACATATCACGACCACTTACCTTCTTAATGCCACCAATGTATTGGGAGTGGTGGTACCAAAACTCGTTTTCTTTTTTATTGCCAGTCAACACAACTTGATCACTGTTAATAATGATCAAGTAATCACCACAATCTTGGTTAGGGGTGAAATCAACTTTATTCTTGCCGCGAATAAGATCGGCCGCTTTAACCGCTAACTTACCGAGAACGAGCCCAGCAGCGTCGACAATATACCACTGTCGTCGCTTGTTGGCCTGTTCTTTTGTTAGCATTGATGTTTTTTGCATATTAACAAAACTGCTTATTATTCTAATTTATTACTTTTCTTCATAAAGCCCTTTGAAGAACGTATCAGCGATCTCCTGTGCCGCTGTTTTTTGAGCTTTTTTAACAACGGTACTACTTAACTGTTCTAACTGTTGTAATTGCAACACTTGGGGTTTATGCGTTAGTTGTTCCACTTTAGCTAGTACCTTAGTTTCATATTCACTCAAAGTAAAGGAACTCAAGAACAGGTTTTGTTGAAAAATTTCTAACAAGGTGGCTTGGGCAGTCGCTTCGGTAAGCGCTTCCTTTCACAACCTTGTTGCTATCTCTTTATCTTCGAAAATTAAGACTAAACCAAAGGGTGATGCTGCTAGAACCTTAATAGCCTTACTTAAATCACTAGCGGTAAACTTATCGGAAAACTGCTTTAAGTAACTGCTGTCTTTAGTTTTTTCTGCTTGGGTGAACTCCCTTTTAAAGCTGTCCAATAACTGTTGGAAGTGGGCTACTAGTTTAGGATCATTGTGCCCATAAACAGAAACGGCAATCACAGCCCGGTTAAGTTTTATTGGGATGGAAACTGGGTTATTATCTGATGGTTCTATTAACTTAATAGGAGCAGGCTTGCTTACAGGCTGTATTAATTTTTTAACAGCGGGCTGAAAAAAATCAGTGTTTTCTACAGAAAGAGGTCTACGACCGCGTTTGCGTACCACCTCTGGTTTAATCGCTAACCCAAATAAATCAGTTGCTGGAGCTGGTGAAGTTTCTGTTGGTTGTTCTACTTCTGTAATTTCTTGCTGTGCTTCTGGTTGCACTATAGTTTGGGGTTGTTCTATTAGTTTAGAAGCAACAGGATCTTTTACGAATGATGAAACTTTAGGTTCTTCTATTACTTTATAAAGAACAGGTTCTTTTGCTGGTAGTTGGGGCTTGGCCTCTTCTACAGTTTTTGAAGGAACGGGTTCTTTTGCTGGTGATTGAGTTTGTACCACTGGCGTATAAGCGGGTATGTGTGGCTCTTTGCTAACGGCATTTTTATAGTGAATCACAATTGCTTTAATCTGATCACTTAAGGATAGTTGTGAAGGACCATAACCATTGTTAGCTTCAATTGCTTGAACCAAAAGAGCATACTGTTTGGGGTGAAAACGAAGCTTTGCCGCCATTGTTTGGTCGCTGGGTTTTACAACTGCAATTTGTCCCGTTTTGACATAACCATATAAATCAACTGTTAGGGCAAACAGTTCCCTGAGAAAGTGAACAAAGTTAATCCCTTGACTTTCAAAGTTGTCAATTAAGTGAAAGGATTGTTTGAGATCACCACTTAAAACTGCTTCAATAAAACCAAACTTTTGTTCCTTGTCAAGCAGGTTAAAGGTCTTTTCAACATCAGCTAAACTAATGGTTTTACTTTCACTAAAGTTACTAATTTGGTCCAACAAGCTCAACCCATCACGCAACGAACCTTGCGCTAAATCAGCTAGTTTGACCAAAGCATCAGTAGTAATCTTAATGCTTTCCTTAGCCGCTACTTCCGCTAACCGTTGCTGGATGAGATCGTTAGTAATTTGTTTAAAGAAGAAGCTTTGGCAACGCGACAAGATCGTGATCGGAATCTTGTTAAATTCGGTGGTGGCAAAAATAAACAAGACATAATCGGGTGCTTCTTCTAAGGTCTTTAAAAGCCCACTCCAAGATTGGGGGGTGAGCATGTGGGCTTCATCCAAAATGTAAACCTTCTTTTTGAACTTAAAGGGTAGGTTAAAGACATTTTCCGCTAGCTCCCTAATGTCGTTAATCCCGTTTTTGGAAGCAGCATCAATTTCAAACACATCAATCGCACTGTTGCTGTTAATCGCTTGGCATGCCTCACACTGGTTGCAAACATCGCCGTTCCAGTTTAAACAGTTAATGGCTTTGGCAATAATCTTGGCAAAGGTCGTTTTTCCCGTACCCCGCTCCCCCGCAAAGATGTAACCGTGGGGTAATTGGTCTTGGGTAATGGCATTAACAATAATGCGTTTAATGCTGTCCTGACCGACAGTATCGCTAAACTTGGTGGGACGGTATTTCTGGTAAAGGACCTTGCGCATGTTTAAGCTTACTTTAAGATTTTAGCGAGATACTGGGCAGTGTAGGACTTATCGACCTGATTTAACAGTTGTTCTGGTGTGCCTTGGGCAATAATTTGTCCACCTTTTTCCCCACCTTCAGGACCCAAATCAATGATGTAGTCCGCCATCTTAATAATATCTAGGTTATGCTCAATAATAATGACACTATCACCGTTTTTAATAATCCGTTGGATCACCGTTAACAGCTTATTAATGTCTTCTATGTGTAGTCCCGTTGAAGGTTCATCCAGGACGTACAAGGTTTTACCAGTTGCCTTTTTCTGGAGAAACTTGGCTAGTTTAATTCGTTGTGCTTCCCCACCTGACAAGAAGGTAACATTAAGACCTAACTGGAGGTATTCCAGACCGACATCACAGAGTAGTTTTAACTTTCTCGCAATATTCGGAATTGCTTTAAAAAAATCATAAGCTTCCTGACATGACATCTGTAACACATCAAAGATCGATTTGCCTAAGTACTTAACTTCCAGGGTTTGGGGATTGTACTTCTTGCCATCACACATTTCACACGTCACATAAACATCGGGTAAAAAGTGCATTTCAATCCGAATGACCCCATCACCAAAACACTTATCACAGCGCCCACCGGCTACATTAAAGGAAAAGCGCGAGTTAGTGTAACCCCGAGCCTTGGCTTCCTTAGTGTTGGCAAACAACTCCCTAATGTCATCAAATACACTGACATAGGTAGCGGGGTTAGAGCGGGGAGTGCGACCGATCGGATCTTGCGACACCACTACCACCTTATCAATGTGGTTGACCCCTATGATTTCCTTATAGGGTGCAGGTTTAACATTCTTATAGTTCACCAAGCGTTCCAAGATCGGTACTAAGGTTTGGTTAATTAAGGTTGATTTCCCTGAACCCGATACCCCGGTCACTAGTACTAATTTATTTAAGGGAATAGTGACATTAATGTTCTTTAAGTTGTTGCCCTTGGCTCCTTTTAAGGTTAAGGTGCGACCATTACCAGCATGCCGGGTTTTTGGTACCTCAATTTGCTTTTTGCCACTGAGATACTGTCCCGTTAAGGAGTCAGTATTTTCCATTACCTCTAGCGGTGTCCCAGCAGCTACTACTTCCCCACCTTGGTTACCCGCTTTAGGACCAATATCAATGAGGTAATCAGCTGCTAGCATGGTTTCACTGTCGTGTTCTACCACGAGTAAGGTGTTGCCCAAATCCCGCATTACCATCATCGTGTTAATTAAGCGATCATTGTCCTTTTGGTGTAGCCCAATTGAGGGTTCATCCAAGACATAAAGGACGCCAGTGAGTTGCGAACCAATTTGGGTGGCTAAGCGAATCCGTTGTGCTTCACCACCTGACAGGGTAGAAGCCCGGCGCGCTAAGTTAAGGTATTCTAAGCCCACATTCTTCAAGAAGGATAAACGGTTAATAATCTCTTTAAGAGCTAACTCACCAATCTTCTTCTGTTCTTGATTCAGTTCTATTTGCAACAAGAATTCAATTGCTTTATCAATCGAGAGTTCCGTAAAGCTAATAATGTCCAACCCACCTAACTTGACACTTAAAGAATTAGTAGTAAGCTTCTTGCCCTGGCACTTCTTACAGGTAATTTCAGACATGTAAGCCGAGTACCATTCACGACTGGCTTGACTACTAGTCTCTAAGTGACGGCGTTTAATTAAGTGAGCCACACCCTCATAGTGTTCAAAGCGCACATTTTTGGCACCTGAGTTTGATACTGTTTTAATCTCAATTGGTTCATCACTACCCTCCAAGATCATCCGTACTAAATCAGTATCCATTTGACCTAAGGGTTGGTCTAGGGGAATTTGATAGTGGTTAATTAAAGACAAGAAGCGCTGTCAATCTAGTGATGTACCGTGCACTATGTTCTTAAAGATGTCAATACCACCTTCATTGATGGACAACAACGGATTGGGAATAATCTTTTCCACATCCGGTTCATAACTAAAACCTAAACCCTTACAGTACTCACACGAACCTAAAGGGGAGTTAAAGGAGAATAAGCGTGGCTCTAGTTCACTAATTGAAAAACCACACTGATCACAACCGTGGTTCTTATTAAAACTAAGTATCTTACCATCTTCCAACAACACTTCAATCCGACCCTCAGTTAGTTTAATCACACCTTCAATCGCATCAACAATCCGACCCAAGGTTTGCTTGTCTTGGTTAATGACAATCCGGTCCACTACCACACTCAAGTTGTGCTTGGTGTTCTTGTCTAACTTAATTTCTTCATCTAAGGTATAGACCTGACCATCAACCCAAACCCGGAGAAAGCCTAACTGTTGGTATTTAGCAAACTCATTAGCAAAGGTACCGCGTTGGTTTTTAATGGTAGGGGCTAAGATTTGAATCCGTGATTTAACCGGTAAGTTAAAGATCTGGTTGGCAATTTGGTTAACGGTTTGGGTTTGAATGGCACCATGACCATTAGGACAAAAGGGTATACCAATCCGCGCTCACAGTAAACGGAGGTAATCGTAAATTTCCGTTACTGTTCCCACAGTGGAGCGGGGATTGTGTGAAGTGGTTTTTTGGTCAATTGAAATGGCTGGGGATAACCCTTCAATCGAATCTACATCCGGTTTATCACTGTTACCGAGAAACTGTCTGGCATATGACGAGAGTGATTCCAAATACCGACGGCGGCCTTCCGCGTAAATGGTGTTAAAGGCAAGTGATGATTTACCCGAACCCGACAACCCCGTAATAACGACAAACTGGTTTTTGGGAATATCGATGTTTACATTCTTCAGGTTGTTTTCACGCGCGCCCTTAACGCGAATAAAATCATTAGTTTTTAGCTCTGGTTTCAAAGTTAATTGTTTGGGTGAAAAAATCCTTTACGAGGTTATTGTGCGGATCGTTAAATAAATATGCAATGCCTTGTTGGTTTTGGTGGGCAATTAACAAACCACGGGTAAAGTTATAAGTGTGTTTGTTAAAGTTGACAAAGGAGTCGACCAACACCAACTGGGGGTGTAACAATAGCTTAAATAACAGGTAAAGTTTAAAGCGTTGCTTGGTGTTAATTTTATTTAAATCGCTAAAGATCATTCAGCTCGGAATACCGTTTTTAAAGGTGTTATCCAACAGCATTTTGAGGTTAGCAGTACGGTTTAGTTTTTTGGGATTTTGCCGGCACTTTTTGAGACAAAAACTAATGACATTCACCATCGCGTTTAAGAGATTAAAGTGCAGCTTATTCGATGCCTGTAAACGGTTTAAATCACACTCTTCTGAATCCACAAAGAAGGTTTTGCGTACCACGTTATTGACTTTACTAATTTGAGTATTGAGCAGCTTATTTAAAGTGCCATAATTTTTTAGCAGTGCTTTAATTTGCTGTTGCTTTTGCTTAATTTCTTTTTTGTACAACCACTGGATTAAACGAATGTCTTGTAAAAGGAGGTGGTAGTGTGCCCGTCACTGTTTGAGCTGGGTTTTTAAGTTAAGTTGGTTTATGGTAGTGGTGTTTGCTTCTGTTAAAGTAGTGGCATTACGATTAGGTAACAACAAGTAAGCTTGAGGGTGGTGCAAGTTGGCTTCGAAGTATTTATTAACTGTTTTATCCACCAGTAACTTTCACTTCGGGGAATTTCAATCGCGGTTTAAGTTGGCCTTTAAGCTGGCAAACTGAGTTTGATTCCGACCTTGGTTAAAGACAAAGTCAACCACACCATGGGTAATAAACTGGTATTTGGTAAAGAAGTGTTGTGTTTGTTTGTGCAAGTTAGTTCATAAAGCACGGCACACAAAGTAGAGTTGGTTCAATTCGTATTTCGTTCAATCTAATTTTTCGTAAGTAGTTAAGAGTTGTTCAAAACAGCGTTCTTGAACAATATTCATTAGGTTAAACGGATCTAAGGTGTGTTTTAACTTAGTGTAGTTAAGCGCTGCTTTAACTGTTTGGATAAAACCCTTAAAAACCGCCTTTTGGTTTACAACCGAATAGCTTTGGCTAACCTCACCCTTATACTGGTTTTGGTTTAACAGAACGTTTTGCTTTAACTCATTGACCAGCTTGTTGTGTTGCTCACAGCGATTTAAGCCCTTAGCGTATTCTTGCAAAAAAGCTAAACGAGATTTAATTAAAAAACCAATCACCGCATCATGAGTCAAAACAATTGGCTTAGTTAACTTTTCCAATTCAAAGCTAATTGCTTGGTATTTTCAGTTAAGTGATTGACCTGGTTTTAGCTGCAAAATTTGCTGGTACAAGCGCTGGATGTTGCTCATTAACTGTTCTACTAGGCGCTCACTTTCAGCAATTGTGCTCACTAACAAATCGGGATTAAGTAAGCGCATTTGTTTCAGTTCCTTTAACAGTCAAATTTTGATTTGGATTCGCTTGGCTACAATCCGTTTTTCCAAAATCCGGGTTTTGTAAAAGTTAAAGATACTCTTGAACTTAATCTTGGTTTGACTCCACCACAAGGACGACATAAAGCGGTTAATCAGCTGCACCGAATTGCGCTGCTGTTTTTTGAAGGCACGCTTAAGCTGATTGGCTTGCTGTTGTGCACGGGTAATTTGCAAATTTTCAAAGTAGGTATTAACCCCGTGTTTGGTTTCATAGCTTTGAACTTTTAAATTGTGCAGTAAACGTTCTCGTTGTCTTGTAAGCGCATAGTACTGTTCAAAAAAACTTTTAGTTAAGTGATCAGGTTGTTCAAAGAAGCTGTTAAAGAAGTGGGTGCTAAATTCCACTAGGTCACGTTCATGGTCTTCCAATAAACCAACAAAGTTGTTGTACTCAAACTGGATCTGGTTAAAGTACACCTTATTGACAAACTTAGTGGTGAAGAACTGACTAAGTGTTTCACTCGTTAACTTATTGGTGTTATCTAGTTCAAACACCACAAACTGGCTTAACTTCTTGAGTGCATTAAAGCCCAAAAATCAGCGCAACTCATTGGGAATTTGCAGTTTTTCAATTAAACGCTGTCGTTTTTTAGGACAAGTACTTTGTCTTAAAAAATCATGAATAGTTTGGTGAAACGGATGGCTTTGTTGCACCGCGGCCAGTTCAATTTTTTCACACAAGTTGGTTTTAACGCGATGCAGCGGAATATAGTTGTGTTGATCCGTTCACAAAAAACCGGAATGGGGCGCTTTTTTGAGTTGTAAAACACTCGACAACGTGTTTCACTGTGCCTTATTGAGGTTATTAACGAGGTAGTTACCGTATTTATGGATGCTGAGGTTGGTGGTTCAGAAGTAGTTAATGTCCAAAAAGGAGTTATGGACCATTAACAAGGACAAATTAACTCACGGTTCTTTCTTAGTGGTAGTGGAAATCTGTTTCAGCATCCCTTAGTCTTATTGATTTTAGTACTAGGGTCATCCCAGTAACAACTAAATAGAAATAATAGTTGACAGGATTAGTGGTGCTTTAGCAGTCATTTTGGTAAATAAATTCATGACAGCTCGTTTAATAGAAACCTTGAGTTCCTTCGATGTGTCTTTGCCTAAACGTCGCTTAATTGTCGTAAAGTCCTTAATGTCGTTAATTTGTTTGCGAATTAACTCTTCCATTTTGGTCTTGAGTTTAACCTGATTGTTACTATCTAGGCTAACTCCTAAAAAGGAATAGGTAATTTCGTTTAGGAACTCACTCTTCTTGGAGTCATAGTAGATGATGATGGTAACCACACCCGCTTCAGACATCTGATCACGCTCAAACATGATGCTGGCCCCTATTTCCTGCAAACCAGCGGAGTCAACACACTTAGGGTTGAGCTTTAATTCCCGTTTTTTGTTGTCAATTTGCTTGTGGTTAATGGCTAGGACTTCACCATTAAAGGGAATTAAGACCTGACTTTGTTCCACACCAGCTTGCTTCATTACCATGGTAAAGTTAATGAAGTCACGGTACAGACCGGATGTGGGAATAATGTACTTAGGTTTTAAGGAAGTTACTAAAAACTTCATGTCTTCATCCGATGCATTGATCGACAAGATTTCCCGACCCAAGTTGTAGTAACTCACCTCATTACGCGCGACATCGTCCAAGATTTGTGCTTCCAACTCTTCATACCCAGCTATGCGGGGAATCATGAAGATAAAGCTATCGGTCTTGCGATACCGTACCCGTTCATCCTCATTAGTACCAATCTTAAACAGCTTGGCATACAACTTATCTGGTGGCGCGGTTAAAACAACAATGGCGTTTGTGGAGTTGTTAATCTCTTCAATCGAAATAGTGTTTAAGTGGGTGTTGTTAAACAGCTTTTGGCGCACAATCGCGTTGAAGAGGTGGACAAATGAATGGGAGTAGATGACAAAGGGGCGGTTTTGCATCCGTGCAATTTGGGCCAAGGTCATCACACTGTAAGCGTTGGAATCATAACAAGCAGCAAATACCCGACCCTTAGCGGAGGCAATAATGCGGTTGAGTTGTTCCAAGGATTTGTGCTTAGGGGTTGTAAAACCAGTGTTACGTCCCACTAGACCAACTCCAGTAATTAACAAAAGGGTCTTGTTCGCTACTTGGGGAATAATCTGATTGAGTTGGTTTTCAAAGGCGATGTTCTTATCATTGAGCACAATGAAGTCATCGACATAGACAATGTACCCATCATCAGTGTGGAGCGCAAAGCCAAACGAGGAAGGAATCGAACTCGATACCTTAAAGGGCGTAATGTTATGGTGCCCGATCTTGACCGTTTCTAACGGTTTAAGCTCGTGGATTTCCAACTCGTCATGGGGAATATTGAGCTTGTTTTCGTGGATCTTAGTTTTAATAACCACAGCGCCAATCGTACTGGTGTAAATCGGAAAGAAACCAACGGTGTGGAATAAGAACTCCAGCGAACCAATGTTTTCCGTTACTGGATTACCGATAAAGATGCCCTTAATGCGGGCCTGGTTTTCCTGGATCCAACTAAAGTCAGGGATGATCTTTTTGACCCCTAAAACAGCGGTTGTTGGTGTCAAACTACCAACGTTAAAAATAAAAACATCGTTATTGATTTCGAGAACAAAACAATTCTTGCCCCTCTCGTCCTGACCACCGAAGGCAAAGAAGTTAATTTTAGCCATGAAACTAACCCCGGAGATTTAAGGTTTTAACTAAATTTCTGTAAGCCTCTAAATCGTTGTGCTTTAAGTACTTCAACAAGCGCTTGCGCTTTGATACTTTCGCGTACAAACCACGTTTGGAAATAAAGTCCTTCTTGTTAGCTAGTAAGTGGTCGGTGAGCTGTTTAATTTGGCTCGTTAAAAGGGACACTTGTACCTGAATACTACCCACGTCCTTGTCGTGGAGTTGTGCGGATTTAATGATCCCGTTTTTATCAATTTGCATGAATTAATACTTGTTGAATTATAACAAAATAAGAAATTAGCCCAGTCAAGGGGTGAACCCCACAAAATCAGCCCCCAAACTTATAATAAATAAACCAAAACAACCACCTTAAGTGGGCTGTTAACTAATTAATTACTAATAACTATCAAAGTCATCTATGGACTCAACTTTTAATGAACTAGGCGTCTCTCCTGCCTTAATTGCCACACTCAAAGATAACAACATTAACCAACCAACTACGATTCAACAACTGGCTATTCCCCAGTTTCTCCAACATCAAAACCTGATTGTCCACTCCCCCACCGGCACTGGTAAAACCGCTGTTTTTGCCATTCCGGTCATTGAAACACTCCTTAAAAAACCAAGTAAAGGTACGACCCAAACATTGGTAGTTGCACCTACCCGTGAACTGGCCGAACAGATTAAAACCACCTTTATTAACTTTGCTAAACACACCCATTTAAAGGTAGTGAGCTTAATTGGTGGCATCCCCATTTGACAACAACTCAAACAACTAGAAAACCAACCAGAAATAGTTGTGGGCACTATGGGGCGGGTGATGGACCTGTTAGAACGGGGTGTAATTAAGTTTGAACACCTAGAACACCTCATTATTGACGAGGTTGACCTAATGCTCGACCGTGGTTTTAAGAGAAAACTGTTTGATCTGTTAGGTCGCATTGAAAAGTTTGAACAAATTGCGGTTTATTCTGCTAGTTACAATGAAGAGACAATTGAAACAGCTAAACAAATTACCAAGAATGGTATTTTCCTAGCAGCACCAGAATTAAAACAAAATGCCCCTGAACCAGATAACAAACTCATTGATCAATTTGTTTGTTATCTTTTTTCTAACCGGAAGAAACAAGCCCTCTATTCTTTGGTATCGCAAACTAGAGCGAAGTCGATTATTGTTTTTTGTGACACCAAGAAGTTAGTTGATGAGCTGTGCATCTTCTTGCGCAAGAATGATGTCAAAACTTATCCACTTCACGGGGACAAAGCGCAGTTCATTCGCGAACGCAACTTAAAGCTGTTTGCTAACACAACTGCTCCTATAGTATTAGTAACAACTGATCTAATCGGTCGTGGTATCCATGTGGAAGGGGTGGACATGGTAGTCAACTATTCCGCTTGTGTAAACTTTGAAACTTATTTGCACCGGATGGGTCGCACAGGACGGAACAACCACAAGGGCTCATGTATTACCTTCTGTACCTCACACGAAAAGCAAGCATTTTTAAAGTTATTAGAACAAGTTAACGACAAGCGCATTAGCCCTCTCCGCCCCATGCGCTTAAGGTTAATTCCCTTGAAGTGCAAAACCCAACCCAAGAAGGGGAAACTTTCCTTACAGTCAGTGCAAAAGATTTACGTCAATCCTCGTTCCAATGGCACCTTTAAACGCGTACCTTTAGCGGGGGATTTGTTTAAAAGCCGGATGCGTCAACCTGAGCGGGACATGCAAAAGAACAAATTGCATGACAGTGATTGACAAAGCAATATGTAAGCTTTGTTACAATTATTTGATATGGCAAAAAAAGACCAACTTACTTTAAGAGGGCCTTTGTATGGCAACAATCGTTCCCACTCCAAGACTATTACGCGCAGAAAGTGGAATGTTAACCTCCAACCATGCAAGGTCAAAACTGCTGATGGCAAGACCACCAGAATCTTAGTTTCTACCAGAACACTGCGCACCTTAAAGAAACACAACCGCCTCTCTTAGTGGCGCGCTAGAGTTAAATTAAACTTAAATCCATATAGTAAATATGGACAAAAAGTACGACATCACTGCTGTTTTAAACGAAGACAGCTCCATGACTGCCATCAGTGATCAGTTTCAAATTACACTCGATGCCCGTCCCAAGCACACCGCTAAGGGTTTTGGTCCTTTAGCTGCTCTGCTCTCTGGTTTAGCTGCTTGTGAATTGGCAACCGCTAACTTGATGGCACCAGCTAAAATGATTACCATCAATAAACTGTTGATGAATGTCACTGGTTCACGCTCAACCAACCCCACCGATGGTTATTTTGGTTTAAGGGAAATTAACCTGCACTGGGAAATCCACTCCCCTAACAGTGAAACGGAAATCAAGGAGTTCATTGACTTTGTTTCCAAACGCTGCCCAGCGCACAATACGCTCCAAGGTGTGAGTCAACTCAAGATTAACGTTAATGTAACTTTAGTGCATTAATTGGGTAAAAAACGAGCGAAAAATGCTGAAAAAATACCTGAGTCTTTCAGGTATTTTTTTATGCAAAATTTGCCCTAAAAAAAGAGTAAATTTGAGAAATTGATCCGTTGTTCATTCCGGGATAATTTTCCCGTATGAAACTACAAATTTTTAATACAAAGCTAGGATTACTAGCAGCTAAGATTTACTGAAAGTCCTGGAGACACCTAGGACTGTCTGAAGATGAAATTGTGTCAATTGCGCTCCACGCCGAGCATGACTCCAAGAAGCGCTATGACCCCAAATACGGTTTGAGCTTTGAGACCTACTTGAAGTTAAATGGTGCTAACTTCATTAAGTCGAGTTACCGTAGCTTAATGAATAAGGTTGATGACTGAATTGACTTTAAGGACATGAAAACACTCGAGGAACAAAACACAGTCTCTTACACTCCTGAAAACTATTTAAGAAGTGTAGAATTCCGTGAAATGGTGCACTTAGCGTTTAAGAAAGCCAAGAATGAGGATGAACGTCAGGTGTTTGCGCTTTATGTAAAGGGTTACAAAAATTTAGAGATTGCTAAAAAACTCAACCTAACGACCCGTAGAGTGCGCTACCTAATTTCAATCTTTAAGGATCACATTAAGGTATTGACTAAACGGTATGGTTACTAGATGAAGATATCCTTCTTCTTGAAGAATTTTTCCACTAGCTTTTCCACCTCCTTGGCGTTCGCTAAGGTTAATGCTTTTTGTGCTAATGTCTGACACTCTGTGTAATTGAGCTTAGCGATAATCATCCGCGCCTTAAACATGGAACTAGCACTCATGGAAAACTCTTTGAGACCCAAACCTAAAAGTAGGGGAATCGCTAACGGTTCACCGGCCATCTCACCACACATTCCCGTTCAAATATCATTCACTTTACCCCCTTCAATGGTGAGGTAAATGAGTTTTAATAAAGCTGGGTTTAAAGGTTGGTAGAGATAACTAACATTCTTATTCATCCGGTCAGCCGCAAAGCTGTACTGGATCAGGTCATTGGTACCAATGGAGAAGAAATCCACGTGGTGACTTAAGGTGTTAGCCGCTAAAACAGCAGCGGGGATTTCGATCATAATCCCGAGCTTAAACTGTTTATTGCCACCCAACTCCTGGTGCACCTGGTTTAACAGTTGCTTGGCTTGCAACAGTTCATCCAAGGTAGCGACCATCGGGAACATAATGCCCAACTGACCGTGATCAGCAGCACGTAGTAAAGCCCGCAATTGTGTTTTAAAGATGTCCTGTTTGTCTAAGGTTAAACGCAAAGCGCGGTAGCCCAAGAAGGGGTTATCTTCGTGAGGAAACTCATAGTAATTAAGCTTCTTGTCCCCACCAATGTCTAGGGTGCGGATAATCACTAAATCACCGTGCGCTTGTTCTAACACTGCTTTGTAAGCCTGGTACTGCGTTTCTTCATCGGGTCATTCTTGACTGTTCATGTAGAGGAACTCAGTGCGGAATAAACCAACCCCGTTGGTATTGTACTGACATGCCAATTCCATGTCTTTCACATTACCAATGTTAGCAGCCACCACAACCGCATGACCATCTAAAGTCACGGTTTCCTTGTTGGTGTACTGCTTGAGTTCTTGTTGGTATTTTTCTTCCAACGCTTTTTGTTTCTTCCACTCGGTAGTGTCCTTGGAGTTTAGGTCTAAGCCAGCTATTCCTTTGCGCCCATCAACCCCAACTATTTGACCGTCTTTGACCTTAGTAGTAATGCCCTTAATGCCCACCACTGCGGGAATTTCCAGTGAACGCGCCATAATGGCAGCGTGACTGGTACGACCACCTATTTCGGTTAAAAAACCCTTGACGTATTTTTTGTTAAGGGTAGCAGTTTGACTCGGCGTTAAGTCATGGGCCACAATAATGACATCACTCTTAATCTTGACCAGGTCATGTGGTTTTTGTCCGGTTAAATAAGCTAATAAGCGTTGGCGCAAGTCCAAAATGTCTGCAGCACGTTCCTGGAAATACTTGTCCTGCATTTGGCTAAACATGGTAGCCGTTTGGCTAAAGACAGTGTCCACGGCTATAACAGGATGAACATTTTGCGCTAACTGTTGTTCTAGTTGCTCGGTGATCGTGGGATCGTTGAGTATTTGAATGTGCGCATCAAAGATCATGCCCGCTTCAGTGTTAATGTTGTTAACGGTAATTTCCTTAATTTCCTCTAAGTCCTTAACGGCTTTTTTGAAAGCTGAGTCCAAGAGTCTTTTGGCCTGCGCCTTCGTTAATTGGTGCTTGAGGTATTTGTTAACAGCGAATTCCGGTGTTTTAACAAGGAAGGCTTTGGCTAGGGCCATGCCATCAGATACACCGATACCAGATAGTTTTTTCATTTGTTTACTTTTTACTTATTAAAAGGGAGTTTGCGGTCATTTCTGCGGGCTGTTTGAGCCCTAAGTAAGCCAAAATGGTTGGGGCAATATTGGCTAGACTAGCACTGTCTTGTTGTAAGTCAATGTTTGTATCAGTACATACAAACGGCACCTTGTTGACAGTGTGTTTGGTAACTGGGTTATTGTTGCTGTCAATCATCTCTTCCGCGTTACCATGGTCAGCAGTTAAGAACAGGGTAATGTGGTTAGCCTTGCAGAAATCAATAATGCGCTGTATTTGCACATCCAAGGCCTCTAAGCCCTGCACACAAGCGGCATAGTTACCAGTATGACCTACCATATCAGGGTTGGCAAAGTTCAACACCGTCAAATCGAACTGGTTAATTTGATTTAGTAATTGATCAGTTATCCCCTTACATGCCATTTCCGGTGCTAAGTCATACGTAGCCACCTTTAACGAAGGTACCATGCACTTAGTTTCATTGGGTAGATCCACTTCCACTCCACCATCAAAGAAGAAGGTTACATGGGCGTACTTTTCCGTTTCTGCAATTCGCAGTTGCTTTAACTTATTGTGCGCAATGACTTCCCCAAAAGTATTGGGAATAGTTTCGGGCGGAAAGACCACGGCACTTGGTTTAATCCCCTCATAGTTCATCATCGTGACAAAGAAGAGGTTGTGCTTTCGTCCTGCTTTAGGGGTGTAGTCATAGTAATCTGTTTGAAAGAGCATGTGTGACATTTGCCGCGCACGATCAGGACGGAAGTTAAAGAAGATCACACTGTCATGATCTTTGAGCGCAAACTGCTCTTTATCTACATTACCATTGACAGCCGGGTACAAAAACTCATCGGTTACCTTTTGGTCATAGGCACTTTGAACATAAGCTACCGGATCAGTAAAGCTAGCCTTACTGTTACCCAAAATGGCATTATAAGCAATCTCTTCTCTGTCCCAGCGCTGGTCACGATCCATACCATAGTAACGCCCACCTAAGGTACCAATGATGACATTGTGGTAATCCTTTAAGAACACCATGAAGGCTTCAAGGTCACTCTTCATCGTGCAAGGTGCTACATCCCGCCCATCACCAAAGAGGTGGAGCACCATTTTGACCTGCGGTGCTGCTAGTTTAATCAAGGCATACAAATGGTCCATGTGACTGTGTACCCCGCCATGGGAAAACAGACCAATTAAGTGCAACTTAGCATTGTTAGCCTTGGCCCTAGCAATCGCCTCTAGTAACACCTGGTTGTGGTGAAAGGCCCCGGTTTTAATGTTTTGGTTAATTAACGATAAACCAGTGTAAACAATTCGACCAGCCCCAATATTGAGGTGACCCACTTCCGAGTTACCGATTTGTCCTTGTGGTAGACCAACGGCTTCCCCCGAAGCTTCTAACATGACACAGGGATAATCCTTAATCAAACTGTCTAAAGCTGGAGTTTTAGCATGGTACACCGCATTACCGTGTTGCTTATTACTAATACCATAACCATCCAAAATGGCTAGTAAGACCTTCTTATGCATATACTTGTGCCATTACTAAAAAGTCGTTGACATCCAAAGATGCCTTGCCCACTAAAAAGCCATCAATTTGCTCGGTTTGTGCTAACTCCTTAATGTTGTCCTTAGCAACCGAACCACCATAGAGAATCGAGGTTTGTTGGGCAATTTCCTTGTTGTACAGTTCACTAAGGTACACGCGAATTTGGGCAATTGTGTTATTAGCAATTTCCGGTGTAGCGGTTTTACCCGTACCAATTGCTCAAATTGGTTCGTAAGCAATCACTAACTGTTTGAGTAAGCTTAGGTCAATGCCTTGCAAGACTTGGGTTAAGTCTTCTTTGAGAAAACTAATTGGTTGACCTTCCGTTTCACCAATACAGAGCACGACTAACATTCCAGCTTGTAAACAGGCTTTTAGTTTTTGATTAATAACTTGCGCTGTTTCGTTAAAGTACTTTCGCCGTTCCGAGTGACCAATAATGACACTGTTGACCTTAATGTCTTGCAGTTGGGTATAGCTCACCGTACCAGTGTAAGAACCACTAGCAATAAAGTTAGCGTCTTGGGCTAACAGTAAGAGGTTATTAGGCAAGACTTTGTTTACCTCGGTTAAGTGGACGTGGACTGGGGCAATACCCAAAACCGCCTTAGTTTTAGCAGGGTGCTGTTTAAATTGCTCCACAAAGGCCAACGCTTGGTGTAAATCCTTATTTGTCTTTCAGTTACCAATTAGGTATTTCGTACGCATACGTTAAAGATTTTATAAGTTTTATTGATTAACCCCAGCGGGAAATACCTGATTAATTTTCCTGGGCTTTAACTGGGTCTTTTACTGGTGGGACTACTTTATCAGGTGCTTTTCTTTCATAGATATAACCATCCAACTTTTTAATGAAGGTAATTGAGAACAGAGCATCCGGATCGACTCGTCTAACAGCTTCTAAAAGTTCGGCCGTTTTCGTAAACAAACAGTTTGTAACTAACACCTGGTTCTTTTGGCGCGAATACCCCCCTTCCACTTCGAACATAGTGACCGCAAAGGACTGGTTACTGCTCAACAAGTAGTTGCGCATTTGTTCCATGTGTTTACCATACACTTCCACTTTGACAAACTGGTATTTAGGAAAGAAGTAGGAGGTAAAGATCCCTAAGATAATGTTCATGAAGATCGTAAACACTAAGTTAGGGGACAAGAAAAAAGCCACTCCAAACGGTTTTTGTCTATCTCCTTGAAAGCCTTGTGCTAGTAAGCTACCGGTAAGGTAAGTGCCAATGGTGTAACCGATCAAGAAACTAAGGGTGTTAACAATAAACAAAATACCACCAATGTCCTTGTGTTTCTTTTCCGAATACCAGAAGGCCAAAAAGTCCAACCCACCACTCGATGCATCAATAATTAGGATAACTGAGTAAAACACAGCTTGAAGAAAACCCCAGATCAGCGCATAGAACATTAACGAAATTTGCTTTTCAGCGGTTTGTTCCCAAAAGATCAGTTGCACCCCTTGGTTGTTAATCGCTTGTTCTAAGCCACCGTTGGCCTTAGTAAGTTCAGTTAAATTAGCAAACAAGAAGAAGTTTTCCACGCCCGGAATGTAAGAGAAGGCAAACCCAAAAACGTTGGAGACTACCACAAAGTAAAGGGTTAACAAGGTAAACTTTTTGGAAATCTTGTACCAACCCAAAACAAATAAGGGAATGTTAATCAGAATTTGACTCAACCAGAAGATGACGTTGTAAATGGTGTCAGCATCCACACTGACCTTGTTCGACCTAATTAAATAATTCGCTAACCGTGCTAAGCCCTGACTAATGGAAGCAATGCCAATGTCATATAATCCAGAAAACTGGACAAACACAACCCCTAACAAGCCCCAAAAGAAGGCAATCACGGAGAGAATTAACAGCTGTAAGTACAGCTTTTTCACCTTGTAAATTGCCTGGAACTTCAGAAACGATCCCGAAAGGTGTACCCGTACCAGATTGCCCTGAATTTTGACGTTTTTATCTTGCATATTCCAAGTCCTCCTTTTTCATCATTTTGCCAATTGCTAACAGGCTTAAATTTAATATTTATTAGGTACAAAAATCGATTACATTAATGGCAACAAAAATAGAGCTCATAAAAGAACTACGTAAAACAACACAAGCGAGCATGATGGACTGTAAAAAGGCTTTGGAACAAAATAACGATGATCTCGAAAAGGCCATTAAGTGATTGAGAGAAAACGGAATTGTTAAATCTGCTAAGAAATTAGGCAAAGTAGCCGCTGATGGTTGCATTGTGTTACACAGTGATCACCACAAGGCAGTGATGGTGGAAATTAATTCTCAAACGGACTTTGTAGCGCGTAGTCAAGAGTTAACGGACTTTGCGCAATTAATGATTAGTGAAGTCTTTAAAAAGGCTACCCCTACCACTACTATTGAAGAAGTAACCCAGTACGAACTCCAAGGCAAAGAAAAGGTAGCAGAACGTTTGGCTTTAGTCGCTTCCAAAACTGATGAAAAGATTGTGTTGCGCCGCTTAATGGTGTTTGAAAGCAAAACTAACCACATCTTTAGCTACCTCCACGCTAACAAACGGATTGGCGTAATCTTGGAAGTGGAAGGCAAGTTTGATGAACAAGATGGTAAACACTTAGCAATGCACATTGCCGCTAACTCACCGCAGTTCATTGATCAGGACAATGTGGATCAAACATGGTTAGCTAACGAAACCAGCATCATTAAGTCCCAAGCCAAACTAGAAGTCCAAGACAATCCGAAGAAAGCAGCGTTCTTAGACAAAACTATTGCTGGACGCGTGAACAAACTCTTAATTGACATTTGTCTGGTAAACCAAAAGTACTTGGTCGATGAATCCAAAACAGTTGGTCAATTCTTAAAGGAAAAGAACAGTAAAGTAATCCATTTTGTGCGCTTTGAAGTTGGTGAAGGCATTGTCAAAGAAGCAGTTGACTTCGCTAGTGAAGTAAGTGCGCAAATGAAAAAATAAGATGCGCCTAAAGATCCTAATCAAACTAAGCGGTGCTGGCATGAGCGCTGATAGTTCCGAACCGTTTTCCAACCAATTTTTAGAAACTGTCATCGCTCAACTAAAACAACTAGTACCCAACTACCAAATAGGCATTGTGATTGGTGGGGGCAACATTATGCGCGGTAAGTCTTGTAGTGACTACAACATTACCGAAATAGCAGGGCACCACTTGGGCATTATGGCCACGGTTATTAACGGCGCTTTTTTAAAGGCTAAGTTTGATAGTCATAAACTTAACAGCACCCTGTTGAGTGCAGTGAGCTGTCCTAGTTTAGCGACCCACATTGTGTCCCAGGCCACGATTGATGATGCCTTTAAAAACCATGACATTGTCATTTTTGCGGGCGGTACTGGTAACCCGTACTTTTCGACTGATACCGCTGTGGCGTTGCGTGCTACCCAAATGCAAGCCGATATTATTCTAATTGGCAAGAATGGTGTTGATGGGGTTTACACTGCGGATCCTAAAAAGGATAAGCATGCTAAGTTTTTAGCTAGCTTAACTTACGCTGAAGCGATTAAGAATGACCTGCAAATCATGGACATTACCGCCTTTACAATGTGTAAGGAAAATAACTTAAAGGTGATCATATTTAACATCAATGCCGAGCATGCCATCATTAAGGCATTGAGCAAACAAGGTAAATACACCTTAATAGAAAAATAAGCAACTGTTTTTTAGATTAATAACATAATTAAAAGCTGGATCGCTGTTTAATCTCCTGCAGCTTTCCAGCTTTTTTATTAGCCATTTTCAAAATAACTTTCTATGCGCTACTTAGACCTCAATATTAAAAGCATCTTAGCTGATTGAGAAATTGCTGATGCGATCCGTGAATTGATTGCCAATGCGATTGATGAACACCGCTTAAGCAACACCGCTTTCCCCGTAATTGAATTGCAAAAAGGCTTTCTAAACTCTTCTTTAGTGATTAAAGATTATGGTAGGGGTATTAAGTCCAATCACTTTATCCAAAACGAAAGTAGGGAAAAGGTTCAAAGTGAAAAAACTATTGGTAAGTTTGGCATTGGTCTCAAAGATGCCATAGCTGTTCTTTTCCGTCATAATGTCAAAGTTAGCTTTACCAGTAGTGAAGGCACTTTTACCCCTGTTGAGCGAATGAAGGAAGGTATGAAAGACGGCACCAAAACAATTCAAATAACGGTGGATGAAACCAAGAAGATTGACAAGGGCACTGACATCCTCATTAGCAAGATTAGTAGAAGTGATTACGAAAAAGCCATTGCTATTTTTTTGGAATTAAGAACTGGCTATCAAAAACTGGCTTCTTCCAAAAAGGGTGATATTTATCGCAGTGAAAATGGTTCAGAGATCTTTCTTAACGGGATGAAAATCGGAACCGATGAAAACTTCTTGTTTTCTTATGACATTAAAGAACCCAATAAGAAATTGCAAAAATCCTTAAACCGGGAGCGTAAAACACTTTCCCGTGATAGCTACCGTGACAATATCATTTCCATTTTGAAGTCCAGCATTAATAAAAATACCCAAACTTTAATTAACCAATTAATTGACAACCGCGATCAGTTTGAAACTGGTGAATGGAGTTTTATTGATGTCAAAAAGTTAGTAATGCAAAACACTAACCGAAAGATTTTGTGAACATCCAATGAAAGCAGTGACATTGCAGCACCTGCTTATCAAACATGAGCACAAGAGGAAGGTTATGAAATTATTTCGATTGGTTCATCACAATATCAAAGTATGGAAAATGATGCCGAATTTAAAAGCTATACCCTCAACGATTTTGGTGATAGATTTGTCAATGAATTCCAAACGGAAGAGGTGCCATTCCACAAATTAACTGAGATCGAAAAAGACAACTGGAATTGGGTAATGGCTAAGGTCAAAGAACTTACCAGGGTTTGAAGTAATTGGAAAAATTTATACAAACACTATGAATTTTCGATCATTAAAAAACATCCTAATGCTGAAGGATTACATAGCAATGGAAGAATTCAAATAGTTAGAAAAATTCTTAATGAACGCTCTCACTTGTTCAACACTATCATGCACGAAATTTGTCATGCAACTAGCTTTAGTCCTGATGTATCAAAAAGATTTGAACAAGGATTAACCAGTGCATTTTATCCAGTTATGAAATTGAAACCAGAATAAAATGGTTTTCTTTTCTATAAATGTCTAGCTAAAACTTTTTTGCCAAACGTTTTTGACCTCAAGTAGAAGCTTGTTCAAAAAATCATCATGGCCTTATTAAAGTTTTTCAACCAAAGTATTGCCCTTCTTTTAATCCAATTGGGCAAATTGTTAAAATTCCCAGATTATGTTTTATCTCGATTTAAACATTCAAAATATTCTTACCAATTGGGAGATAGCTGATGCTGTTCGCGAACTGATCGCTAACGCCATTGATGAGCACATTTAACCGAAACCCAATTCCCTAAAATTGAATTTAACGAAAACAATTCAAGCTCAACATTAATAATTCAAGACTTTGGTCGCGGCATTAAAATCGAACACTTTATCCAAAACGAAAGTAAGGAAAAGGTTCAAAGTGAAAAAACTATTGGTAAGTTTGGCACTGTTTTAAAGGATGCAATTGCTGTTTTATTTCGCACTAATATCAAAGTGAGTTTTACAAGTCAGCAAGGTACTTTTATTCCTGTAGAACGGAGTAAAACTGAACTGGAAGGTAGCATCAAAACAATTCAAATGGAAGTTGATCCAGCTGAACAAATTGAAAAAGCGGGTACCTTAATCACAATTGAAGGATTGAAAAAAGATGACTATGACAAAGCTATTGTTAATTTCTTAGCTCTACGGGAAGATTTGCAATTATTAGCTGCTTCACCCAAAGGCGATGTTTACCGTAATACATCAGGTAACGGCGCAGAAATTTTTCTCAACGGGATGAAAATTGCAACCGATGAAGACTTTCTCTTTTCCTATCACATTAAAGAACCTAACAAAAAGTTACAGCGTTCCCTTAACCGGGAAAATAAAAATTTACCAAGAGATTGTTATCGTGAAAACATCATCACCATTCTTAAGTCCAACATTAACAATAGAACACAAACACTTATTGATGAATTAATAGATTCACGTGACCAATATGACAATGGCGAGTGGAGTTTTATTGATGTCAAAAAACTAATTGGTTTAAACACAAACAGAAATATTTTGTGAGCCGATAGCAGTAGTAAAAACATAGAAAAACTAATTTATTAACTTTATGGAATGGATACCAAAAAATATGAAATTTTGGCGTTAAATTCCTTGCAATACAGAAGCATGGAAAACGATGATCGTTTAAAAAAGCAAACACTGATGCATGTAAGTGAAAAGTTAAAACAACAAAGAATTGAAGAAGAGGCTGAGAAAATCAAAGTTAAAGAACAAAAGCCACGAAAACGATTTGAGGAAGAAGATTTGCCAATAGAAGATTTAAATCCAATTGAAAGAGAAGGTTGGGATTGAGCGATGGAAAAGGCCAGAGAATTATGTGGTTTTATAAGAGGATGGGAAAAACTTTATGAAGAATATCAGTTTGTTCTAATGGAAAAGAATCACAAATATGTCGGGCTTTGTTACACCGATCAAAAAATTATCAAACTCTCAAGAGAAATATTGAAAGATGAATACAGTTTATTGAATACCTTAGTGCATGAAATTTGTCATGCAACAACTAATGGTCGAGACGGAACGAAGAAGTTTGAAAGGGGTCTAACTGATGCTTTTCATCCATTATTTAAACTAGGGCAAAGTAAATAATTAGTCCGTGTTTTACATTGTCGTTGAATTTCCGCAACCGCTTTCCATTAAAATAATTTAACGCCTGAATAAAGTGATGAGTCCAGAAAAATACCTGAACTTCTTTAAAGAAACCGCTGATAAAAAGTTCCAATGACTCAAAGAAGAATTGAGCAAAATCCGTACTGGTAGACCTAATCCAAAACTGTTTGATAATATCCTTGTGGAAAGCTATGGTGACAGAATGCCAATGGTAGCATTAGCACAAATTGCGGTAAATCCACCGCGGGAAATTGTGATCAAGCCGTTTGACGTTAAAAACAATATTAATGCCATCTACAGCGAAATTCAACGTGCTAACTTAGGTGTACAACCAGTGATTGACGGAGATAAAATTCGCATTAACTTTCCCCCAATGACCCAAGAAAGTCGTTTGGAAAGCATTAAGCAAGCTAAAAAAGTAGTGGAACAAATCCACCAAGAGTTGCGCAGTGTGCGACGTGATACCCTTCAAATGATTAAGAAGGATGATCATAAAGACGAAGACTTTGAAGAATTCTTAAAAGAAGAAGTAGAAAAAGTTAACAAGCAGTATATTGCACAGTTAGAAACTATTCAAAAACAAAAAGAAAAAGAACTATTAGTGGTCTAATGGATCTGAAAGATAATTCCTTCGCTAAAAAACGATCGACAGTGTTCGTTGTTTTGCTCATTGTCTTTTGTTTTTTTTTAATGTTTTCAGCCTTTGCTGACGGCTTTAACTTTTGATCGCCTTGGTCAACTGATTTCAATTCACGCGTAATTCACATTAAAAGTGACGGTAATATAGAAACCACTAGTATTGTCAGCCATGAGTTGCATCCTGACTTTAAGGCAGTCCGTTTTGCTTTTGGCTTAGTGATAGTTTTATTTATTAGCGTTATTGCCGTTTTAATGAATTGGGAGTTGTCTAACAGTATCTTTAAAAACTATGAAAAGCTCAACTTATCCTTATCACTACTTAGTGGAATTATGGTTTCAGGTGGGATGATTCCAACCTTTTTCGTAATCTATTTCCGTGAATGAAATGCGACCGTTAACTGAATTTGAACTGCCTCGTTGGCTGGCATGATTGTCTTTTTATGGGCAGTGTACATGATTAGCACTTCCTTCATAAAGATCCGACCATCATTGCAAGTGATTTATAGCCTAGGTGCAGTGATCTGTTTCATCGCTTGCATAGGTACAATTTACTTTTCCGTAATTAGGGGATGGACTACCATTTTTCTCTTAATTGCTATTGGCGTTTGCACTGATACCTTTGCTTATCTGTTTGGTAAACGTTTTGGAAAAAATCCCCTCATTAAAATTTCTCCCTCAAAAACTTGGGAAGGTGCTTTCTTTGGGGTTACTGGTACAGTTTTAACCATTTCTATTATTTGTGTTTTGTACTCGATCCCTAACTATGTAAGACAACCTAGCATAAAGGATGCAAGTAAGACAGCACTACAAACACCACAAAACTATGATGTGCATAACCTTATTACCAATGTCTTTCTCATCTCCTTCATTTCCGGTGGTAGTACCTTTTACATCTACTGGTGAGTTAGCACATTAGCACTGATATTTACTGCTTCCATTTTTGCCATAGGTGGTGATTTATTCTTTAGTTACATTAAAAGACTCACAAAAATTAAGGATTTTTCGAAGTTATTGGGTAAACACGGTGGTATCTTAGACCGGTTTGATTCCAGCTCTTTTTTAATTAGTTTTTTCTTTATTTACCACGTAATTGCGGGAATCTCTTCTAACCAAAGATTGCTTATGGAACCAAACACTTATTTCAGTGCTGTAAGTTAGTTTTTAGAATTTAAAGGAAATGACTCCTAAATTAAAGCTTAACACTAACAGTAATTGAACTAAGAAAACCCTTGGCAGCTTATTTGAACTCAAGAAGGGTGAAATGCTCGAAAAAGAGCTATTGGCGCCCGATGGTAAATACGAATACTTTAACGGTGGTATTAAAGCCTCTGGACGCACCAATGAGTTCAATACCTTTAAGAACACCATTAGCATTATCATAGGCGGTTCTTGTGGTTATGTCAGATTAGCCGATAAGGATTACTTCTGTGGCCAGAGTAGCTGTACCCTAACTGTTTTAGATCCCCTGGAAATTGACCTCAAGTTTGCTTATTATGCACTTAAATCACAAGAGGAAAAAATTACCAGTTTAGCTAGCGGAACTACCATTAAAAACATTCGCCTAAGTGATTTAAAGGATTTACCTATTCCGCTTGTTAAAAGCATTCAGGATCAACGAACTATTGCACATGCTTTAAGTGTGTTTGACCTTCGCATCGAACACCTCAATGAATTAATTGAAGTAAACCGTAAGCTCAGAGACGAGTATGCCCACAAATTATTTACCTTAGATCCAGACTTCTTAACACACTGAAACCTACATGAACTCCATGAACAAATGGGTGAAATTAGTTTAGGTGAAGTGTTTCACTTAAAGAGTGGTAAATACTTAAAGGCTGATGAGCGCTTTGAGGATGGCAAATTCCCTTATTATGGTGCTGGTATTGAAAGCACTAGCTTTGTCAATGAACCCAACACCAAAGGTGATACTCTTTCAATGATTGCTAACGGTTACTCCATCGGAAACATTCGCTACCATACTATTCCTTGGTTTAATGGTACTGGTGGTATTGCCATGGAAGCATTAAAACCTAATGAAACCTATGTGCCGTTCTTTTACTGTGCTCTAAAATACATGCAAAAGGATCTAAAGGAACGCTTTAAACGTGATGAATCACCGTTTATTTCCTTAAAACTAGCCGGTGAAATTAAGGTTCCTTTCGTTAAATCGTTTGCACTCCAAAGAAAAGCCGGTAAGATTATTTACTTGTTAGATAAAACTCTTGAAGAATACAAAGAAGAAGCTAAGTCTTTAATCAGTATTCGCGATAACTTATTGGGCAAATTGTTCCCAACACTATCTTAAAGCAAAGCAAATAAGGTGTAAGGGAAAAGGTTAACACCTTCGTTATCAAAGCTCTGGTCTTTGAGACTGCTGGTTAAGCTATTCTCCAATAACTTCTTCATTAGATCACTCGTACTAGAAACTTGAATTCTTAACTTTGTGTTAAGTTTGATGTTACTGGTAAAACGGTCAAATGAAGCGCGGCCAAAGATGCCTTGAGTAGCTGGACTATATGTAAAAGTGGCTTCTAGAGCTTCATTTTGACCCATACTCATGGTTAAAGAATTTTGAGCATCTTTTTTCTTAGTTTCATCATCCTTAAAAATATTCTCTATATGGCTCATCGCCTTGGTTGGATCAATTTTTAAATCTGCCTTAATCTTAACCGTAGCCTCAAATTGCTTATTCGATTCAACTTTGTAGGTAAACTCCTTCACTTCTTGCTTGCTAACAGTGATGGCTTGTTCAGCTAATAATTGAATCTCAGTTGTTGAACGTTCACTTAACTTTAAACCCTTAAGCGTAGCATTGGGTTGAACACTGTTTTGGTTACTACCGTTGCCGAAGGATTTAGCAAAGCGGCCTACTTCAGTTTGCCAACGTTCTAATATTCTCTGTGAAAGCAAAGTGTACCTTCAACCTTCAAACAAGAGGTTTAAGGTACCTTTAGGATTTAAGCCGTAACTTTCGCGTAAAGTTGTAATTAAGGTACGTTTATTTTTCGTTAGTTCTGTGTTGCGATCTGCTAGGTTTTGCAGTTCTGATAAAGATTTAAGTTCAACGCTGGCAACTAGAGCAGAACAACTGCTTAAAATAAGCGCTAAACTAGGGATACTAGCTCAAATTCACTTCCTTAATTTAAGCGCCATACTCCCTAACATGCAGTAATTAAACAATTGTAAAAATTACCGCAAATACTGTAAAAGGACTAAAAGAATTAAAGCAGAGCGTAGTAAAAAAACGGTAATAAATTAAATAACTTACCTGTTAATTGACGACTTTTCATCTTTTTGACCTGTTCGTTGCTTTCTAATAATTCATTCAGCTTTGTGTGAGAAGTTGTTACATCAATCTTGACATTAGCGGGAAAGTTAATCGAACTAGTTGGTACATCAAAGGATTTATCAGAGAAGTTTCTAGCACCTCCAGTTGGTGGGGTATAGGTAAAAATAAAGTTACCAGTGAATTTGGCTTTTTCACCAGTGTATTCACTTTCATCAATACTGTTTTTACCATTGCTACCTTTACCTCCCGAACTATTACCCTCTAAGAATGATTTAATCAATGCTTTGCTTGATTCATCAATCTTTCCATCCATTTTGATTTGAATATTTACCTGAAACTTTTTTGGACTGTTTCAACTGTAAGAAAAACCACTAACTGTTTGCTTTTCAACTTTAAATACTTTAGCGATTTCTGAACTAATACCAGGAAAAGTGTCTGCTAAAAATAAACTTTTTTCACTAATGTTAGGGGTAATATCGTCCTTCTTGCTAGTAGAACCAAAAGCGCTAGCAAAGCGTGAAGCATCTAAATTTCTTTCTAAAAGCTTTTGATCCATTAAGGTGTACTTTCAACCTGCTAAAAGAGCGTTAACTGTTTTGTTAGGATCAGAGTTATAGCCATCTTTCAGAATGTTGATAACGTTCTTTTTGTTCAATTCACCTTTATTTGCTTCAAAGTATTTTTGCGCGCTACTTAAACTCGATATAACCTGGCTAGTTGCAGAGGAACAAGCGCTCAGCAATAAACTAGATCCTAATAAAGGAATTAATAACAGTTTAAGTTTCATTAGAGAATGGAATAAAGCAAGTAAGGTAAAAGATCAATTGATTGGTCTTGGAAAGTTTGACTGGACAAATTATTAGTAAAGGTAGTACTTTGAAGAATCTTTTTCATGATTTCGTCTTTACCAACCATATCTATTTTTAAGGTGGTATTGAAATTAATTTTCTTCATTAAAGTACGAAGTTCTTTCTAGAGAAAAGATTTGATTCAGGTACAGTATAGGTAAAAATAAGATCTGCAGAAAGAGTGCCACTTTCTTGTTTCAAATCACCATCATTTAAACCAATTTGTTGTAGATAAGATTGAACTTGACTTTTTTGGTATTTTCCTTGAGCATTAAGTTTTACTTGAACTTTAAAACTAGTTCGACTTTTGTATTGGATATTAAATGCTTCTACTTTTTGCTCATTTAGCGAAATAACATTGTTGATAATATTAGCAGTGCTTGGTTCAAATCGTTTATCAAGTCTTAAACCTTTAACACTAGCATTAGGCTCAACATCTTCATTACTTTTTCCAGAGCCAAAAGCTTTGACAAATCTAGAAGGGTCTTTGCTCTCCAAAATTTTGGAATCTAATAAGGTAAATTTCCAAGCGTCTAATAAAACTTTGGTAGTTCCTTCAGGATTATTTTCAAATGCTTTTTGCAGTGACTCCACAAAAGCTTTCTTGTCTTGGGTTAGACTGGTATTTTGATTAACGCCTTCCCTCAATTGATTCAAACTTGTTAAATTAGTTTGAAAATCAGAATTAGAACAAGCAGTTAAAGCTGTTCCGAACAACATGAATAAAGCACCTAATGAAGCTTTGGGTAATAGTCTTTTCTTATTCATCTTTAAAGTAGAGCATAGTAAAAGAAGGGTAGCAAGTCAATATTTCTTCCATTAAATGTACGACTTTTCATTCGCTTAACTTGATCGTTTTTCTCAAGCAGTTCATTTAGTTTATTATGCGAAGTGTTCACATCAATCTTTACATCAGCAGGGAAATTGATCATATTGGTGATAACATCAAATGATTTATCGGTTAAAGATCTAGTACCTCCAGTTGGTGGAGTATAGGAAAAGATAAAGTCTGCAGTGAATTTAGCTTTATCTCCAGTATATTCACTTTCTTCTACGCTATTACTTCCATTTGAATCTTTGTTACTCAAAAAAGACTTAATTGTTTCCTTACTCTTTTCATTAATCATCCCTTCCATCTTTATGTGGATGTTTACTTTAAAACTTTTAGGGCTGGTTCAGCTGTAAGAAAAACCACTAACAATTTGTTTTTGTACATTAAACACTTCAGCAATTTGACTTCCTAAACCAGTAAAAGTTTCATTTAAAAATAAACCTTTCTCGCTAATATTCGGAGTAACGTCATCTTTATTTTTATTCGGGCCAAAAGTATTTTTAAAACGAGATGGATCTAAATTATTTTCCAACAATTTTTGATCCATTAAGGTGTACTTCCAGCCTGCTAGTAAGGCGTTAACTGTTTTGTTAGGATCAGAGTTATAGCCATCTTTCAGAATGTTGATAACGTTATTTTTATTCAACTCACCTTTATTTGCTTCAAAGTATTTTTGAGCGCTACTTAAACTCGATATAACCTGGCTGGTAGTGGAAGAACATGCACTTAAAATAATGCCGGAAGCTAAAAGAGGAATTAAATAAAGTTTAAGTTTCATTAGAGTATGGAGTAAAGCACGTAAGGCAAGAGATTTATCGATTGGTCTTGAAATTTTTGATCAGCTAGTTGCTTTACAAAACTACTTTGTTGCAATAATTTAGTCATCACACTATCTTTGCCTATAATTTGTATTCGTAAATTGGTATTAAAGTTGATGGCTCTTGTTAGTTTGTCAAATTTACTTGCAGAAAAGAAACTGGCAGCAGGTGGCATATAGGTGTAAATTATTTCAGCTTCTAATGTACCTGTATCATTTAGATCACCATCTGATAAACCTACTTGACTTAAATAAGTTTTTACAGTATCCTTTTTGTAATTACCTTTAGCATTAATTTTTACTTGAACTCTAAAGTTTTTAGGATCTTTGTATTGAATGCTAAAAGCTTCTACTTTTTGTTTGTCCAGTTTAATTACGTTATTAATTATATTGGCAGTGTCTTGTGTGTATCTTTCCACCAAGCGTAAACCCCTTACTCCAGCACTAGGCTCTACATCTTCAATGCTTCTGCCAGAACCAAAAGATTTACTAAATTTGGAAAAATCTTGTTTTTCTAAAATTTGAGCGTCTAAAAGCGTAAACTTTCAAGCGTCTAATAAAACCCTAGTAGTGCCTTCTGGATTATTTTCAAAAGCTGATTTTAAGGCTGTAACTAAATCAGCTTTATTTTGGGTTAAATTAGTATTTTTACTTGCACTGGTTCTTAATTGATTCAAGCTAGTAAGATTTGCTTCAAAACCAGAATTAGAACAAGCAGTTAAAGCTGTTCCAAACAACATAAATAAAGCGCCTAAGCTTGTCTTATTTCAAATTTTCTTTTTCATATTTTTAGAGTAAGGCATAAAAATAGAAGGGCAATAGATCAAATGATTTACCAGTAAACGAACGACTTTTAATTTTTTTAACATGTTCGTTGTTTTGCATTAGGATATTGAGTTTTTCATGAGTTGTGCTTGCATCAATTTGAAGATTAGCAGGGAAATTAATTGTTCCAGTGATTGGATCAAAGTTACTAAATGCAAATCTTCTTCCATCTGTAGGCGGAGTAAAAGTGAATATGAATGTGCCATCAAAGTTTGCATTTCCATTATTAAAATCATTTTCAGTAACATTGCCATTATTTCCAGAGCTCAAGAAAGATTTAATTAAAGAATTACTTTCTTCACCACTGCCAACAATGCCTTGCATCATAAAAGATATAACTACTTTAAAGTTAGTGGTGCTAGATCAGCTGTAAGAAAATCCAGTTACTTTTTGGCTTTGCACTCCAAGTACTTTAACTATTTGTTGACTTACACCACTGTAAGTTTCATCTAAAAATAAACCTTTCTCACTAATACTTGGAATGACATCATCTTTGCCTTTGTTAGTACCAAATGCTTTTGTAAAGCGACTAGCATCTAGATTTCTTTCCAGTATTTTTTGATCCATTAAGGTGTACTTTCAACCAGCAAAAAGTGAATTAACTGTAGCTTTTGAATCAGTACTGTAACCTTCTTTTAAAAGATTAATTACATTCTTTTTGATTAGTTCACTTTTGTGAGCATCAAAGTATTTTTGCGCACTACTTAAACTAGAAATAACATTGGTGGCAGTTGATGAACAGGCACTTAAAGCAGTGCCGAACAATAAAAATAAAGCATCTAATGGAGTTCTGAATAATAGCCTTTTCTTATTCATCTTTAAAGTAGTGCGTAGTAAAAGAACGGTAACAAGTCAAAGGTTTTACCAGTAAACTTACGGCTTTTAATCTGTTTTACTTGTTCATTGGCTTCCATTAAAGTATTTAACTTTTGGTGAGTAGTGCTTACATCTATTTGTAAGTCTGCTGGAAAGTTAATCTTAGTTGTAAGAGGGTCAAAAGAAGTGGCACCAAATTTTCTACTTTGAGTAGGAGGTGTATAAGTAAAAGTAAAGAAACCATCGAAATTAGCAGAATCACCATTAAAGTCAGTTTCTTTTACTCCGCCATTTTGATTTGATCCATTGTTATCAGAACTTAAAAAAGATTTAATTAATTGTTGACTCTCTGAATCACTTCCAACTTTTCCCTTCATACTAATTTGAATTTGTACTTTAAAGTCAGAGTTACTGGATCACTTGTAATTAAATTGTTTAACAGTTTGACTTTGTACACCTAAGACTTTGGCAATTTGAATACTAAAGCCAGTGTAAGTTTCGTTTAAGTACAATCCCTTTTCACTAACACTAGGAGTTACATCGCCTTTATCGCGTCCGCTACCAAAAGCTTTGATAAAGCGACTATCAACTTCTTTAGAAAGTAATTGCTGGTCCATAAGTGTGTACTTTCAACCAGCTAGCAAAGCATGGACTGCCTTATTAGCATCAGATTCATAACCTTCCTTTAAGATTTTGATGACATTCTTTTTGTTGAGTTCACCTTGACTGGATTCAAAGTATTTTTGAGCACTACTTAAACTAGAAATTACTTGAGTAGCTGCTGAAGAACAAGCGCTTAACAACAAACTAGAACCTAAAAGAGGAATTAATAGAAATTTAAGTTTCAGTTTCATTAGAGGATGGAGTAAAGTACGTAAGGCAGTAAATTAACTGCTTGGTTTGGGTAATTAACACTGGCAAGATTATTAGTGAAGGCACTACTGTTAAGAATCTTTCTCATTACTTCGTCTTTACCAACCATTTCTAATTTCAGATTGGTATTGAAATTAATCGATTTAGTTAACTTGTCAAAATTACTCTTAGAAAACAAATTTGAAGCAGGTGGAGTGTAAGTGAAGATTAAATCAGCTTCTAAAGTGCCTTCTTCCTTATCGCCCAGTTCATTATCTTGAAGACCAATTTGTGACTTATATTTCTCGACTTCACTCTTTTTGTAATTACCTTTTGCATTCAGCTTAATCTGAATAGTGAAATCACGACTACTTTTAAAGCGAATATTAAAAACCTCTACTTTTTGTTGATCCAATAAAATTGCATTTTTAATAAGACTAGCTGTGTTATCACCAAAACGTTCAACAAATCTTAAACCACGTACGCCAGCACTAGGTTCTATGTCTTCCTTACTTTTACCGCTACCAAATGCTTTAACAAATTTAACTGGATCTTGTTTTTCCAAAATCTTCGCATCATAAAGACTAAACTTTCAGGCATCTAGTAAAACCTTAGTGGTACCTTCTGAATTATTTTCAAAGGCAGTTTTTAAAGCATCAATTAAGGTATTTTTGTCCTGAGTTAAATTAATGTCTTTATTGGCTGAAGTTCTCAATTGGTTCAAACTAGTTAAGTTAGCTTGAAAGTCATAGTTAGAACAAGCAGTAAACGCTGTTCCTAATAAAAGAAACAAAGAACCAAAAGCGGTTTTAGATCAAATACTTTTTTTATTCATGCTTCGAAATAAGGTTTAAAAATTAGAGAAGGGCGTAGTAAAAAAAAGGCAAAAGATTAAATGATTTGCCTTTAAAAGAGCGGTTCTTAATCATTCCTACCTGAGTATTAGTTGTCATTAAAGTGTTTAACTTTTCATGACTAGTACTTACCTCAATTTGGAGATCAGCAGGAAAATTGATGGTTCCAGTAATTGGATCAAAGTTGCTGGACGCAAGTCTTCTGCCATCGGAAGGTTGAGTGTAAGTAAAGATAAAATTGCCATCAAAGTTAGCTTGGTCACCTTCAAAATCTTTTTCAGTTACACCACCATTTTGATTATTGCTTCCACTGCCTTTGCTATCAGTACCTAAAAAAGACTTAATTAAAGTTTGACTTGTGCCATCACTACCAACTTTTCCTTTCATTTTGATTAAGATTTTGACCTCAAACTTGGTTTTGCTAGTTCAGCTATAAGAAAAACCAGTTACCTTTTGGCTTTGGACACCAAGAACTTGAGCGATTTGACTACTTACACCGGGATAAGTTTCTGCAAGATATAAACCTTTTTCACTGATGTTCGGGGTAACATCATCTTTAGTGTTAGAACCAAAAGCTTTAGAAAAACGGGAAGGATCCATTGGGTTCTCCAATAACTTTTGATCCAGCAAGCTGTATTTTCAGCCAGCTAATAAAGAATTAACTGTTGACTTGGGATCAGAATTATAACTATCCTTCAAAATGGTTACTAGATTACGTTTATTAAGTTCTTTTTTGTTGGCACTAAAGTACTTCTGGGCACTACTAAAACTGGAAATTACTTCTGTGGTGGCAGAGGAGCAGGCACTTAAAGCTGTGCCAAACAGGAAAAACAAAGAACCTAGACTGGTCTTTGACAAAATCGATTTTTTGTTCATTTTTAAAAAAGTAAATTTTTTAGTTGATTTATTGATTAGTCAGATGGTTTAAAAATACTTGTTTACAAACAATGAGATAAGAGGAGATTTATCCTCTTATTTAGTATGAGTTAACGCAATTCAAAGAAAAGCGAGCGGTATTTAAAAAAACAGAAGTAATCACTAAGTTTGGAATTAATTACCTCCTTTTCAATGACAACTACAATCTCTAACTTAGGCGTTTCTTCTATTACTTCTGGTTGATTGTCTTCTTCTATGCTTGGTTGTTCAATTAAAAGGCCAATAAAATTATGAACTTGGCGTTCAATAAAGAAAACAAAGTTGTTTAAAGAACTAAAATCGTTTTTAATACTTCTTCAAGCTTTAATTAACAGATTAAAGGTATAGTAGTTATTTAATGAGTGGGTGACACGAAAATTAAATAACTTAAATAATGTTTTCGAAAGAAAAACAAGACCGTTAATTAAAAACAAGGAGACTAGTAAGATAGCTAGTACATTTGCTTTAAATTGGCTTTTCATGAAGAAGAAGGTTGGTTAAAATTGAAAGCTTTTTGAACTAAACAAACCCAACAGCTTGACTGAAACTAAATTAGTTCTCGTCTAATTGACCATAATTTTAAAACTTTTAATTGTTGCTTATTCCGCTAATAAGCTCGTTTAACTAGCTCGATTCAAATAAGTAAGATGGCAAAATTAACTAAATTGGCATTAATCAATATTCAACTGTTATTCTAAACTGACTTTTAGCTAAAAAAGTAGATCGATTAAGTTAACTGATTCTTAATAAAAAAGCTTTCATTTTTCAACCAAAAATTATTTTTCAATTGAATGTCTTAAAAAACGAAAAAATCTTATTTCTTGTTCTGTTCGATCATCGAAATGATTTTCAAACTCTTCTCTGTCCGCATTACTTTGACCAATATATTCCCAACTTGATGTTCCATAAAGAATTATTTCTCCGTGTTCCAAATTAAAACTATTGTTGCATTTGGTAGTTTTATCGTGATCTTTTCATAAGAAGTTTTTAAAGATTTAACTAGATTTGTATGGCCTGTTTCTGTTTCAATTTCTTCAGACCAATTTAAATCTTCGTTAGTATTGTTTTAATCAAACTTTATATCTCTGATTTGACATTGGAACAAGCACTTAAAGCTGTATCGCACAAAATAAATAAAGCTCCTAGACTTGTTTTAGATAAAACTTTACTCTTACACATAACGAAGATAGATGAATTAAATGTTGATCCATTTTGCTTTTTTTGGAAAAGACGTGCTTGAAAACTTCAAAATCACTTAATTTTTAGGTTTCCTGGTTTGTAATTTTGAAGTAAAAAGCTTTTAAAAATTTTTAAAAAAGTTTTTTATTTAACTAAAAAGTTTTGAGGAATTAAGTAAATCTTAATAAGTTATCTAATTACGTAAACTAAATAACTAAAAAGGTTGATTTTGTAGTCATCAATTTGAAAAGCTCTTGCTCTACCAAAAACACTTAATTCAAAAAGTTTTTTGCTAACTTCGTCTTTTCCTTCTAATCTCCAGCTAAATGAAACATAAAAGTCGATTGCTTCTGAATCAGGTTTAAAGTTTTCTGCTGCAAATGGATGGTTGGGAGAAGCTTTAAAAGTAAAAAAGAGATCAAAATAAAGCTTTTCACCTTTTTTTGGAGCGAAACCTATTGAAAAATCATTGCCATACCATGTAAAGGTATAGTTCAATTTGGCAACAACTGAAAAATCACTAAGGCTTTGGTAATTCAAATTGAAATACTCAACCTTTTGTTCGATACTAAAGAAACTAGTGCTTAAGGAAATTTTCAATTAGTTCAATTAGTTTCAGAACTCTCTATTTGACCGTTTTCTACACTTTGCTTCCAGGCATTCAAGAGTACACTAGCTGTTTTGCTAGGGTTAGTTTCGTAAGACTTCTTTAAAGAACTTATTAAATTCTTCTTTCCCTCAGAAGTACTAACTGATTCAGACAAATCAAGGTCTTGATCGGTGGTTTCCTTAATAAGACTTTTCAAATTAGCTCCAACATTGACGCTAGAACAAGCGCTAAATAATAAAGCTGAAAATAATAACGGAGTTGATAGATATTTAAATTTCATTAGAGGACTGAATAAACCAGATATAGCAGTAAATTTACTTTGTAATCAAAGTAAATTTTATCTACCAATTTTCCAATAGAACCATTATCTAAAAGTTTTTTGGTTATTTCATCCTTAGGAAAAACTTCTAGTCTCATTGTTGTTTAAAAATCAATGGAATATCACAAATTCCCTAATTTATTATCAGAGTTTTTAACAATACTTGTCTCTAGAATTGGTTTGTTTTTAAATGGTTTGTATGTGTAAATAAGATCAAAATTAAGCAAGTAGTTGCCAAAGCCCGACATTATTAACCCTCCTTGATTTGGTTTTTGATTAATATCAGCATTAAAAGTTGTGTTCATTTTAACAACAACCTTAAAATCATTTTTATTGTTTGCCTGAATGTCAAACAATTCAACTTTTTCGCTAATTGTTATTGATTTCTTGTGGAATTAAATGAATGGATTGATAAAAAAGGTTAAACATTACTTCTACCTTCTTCTCTCATAAGACTATTAAATCATTTGGAATAATACCAGTACCACAGGTGCTAAACAATAAAACTGCTGATAAAAATGGAACTGAAAGGTACTTAAATTTAATTCCTTAATTCTGCTTTTTTTGCAAAAAGACCTTTTGACACCTTAAGCAAAGAATAAAAGTTTTATTCTTAAGATAAGGACTAAACCAAAAATGAGAAAGAAATTAGCAAAAGTAAAAGTTCACATTCAGTCTTTGGATTCATTGCTTTCATCAATGACGATGCCGATTATTGGCATCTTTATTGCTTGGGGACTTTTAGCTTCTTTTTTTATTCCATCTGGTTGAACACCTGATAAAAACCTAGCTTTAATGGTGGAATAGGAATCCAATATGTGATTCCGACCATAATTGTTTTTTTGGTGGTAAAAAGATTTTGAAATACGTGGTGGTGTGATTGCTGTCATTATTGCCATTGCTGTAATAGCTGCTGGTCAAACCGAAGCATTTACAAAAATTGTTGGTCAAAAATCAGTAATGTTTTTATGTGTGATGATATTCGTCCCAATTGCAGCGCTAATCTTAAAACACACTGAAAAGTTTTGAATTCACCGTATTAAAAGTGGCTTTGAAATGCTGGTAAATAATTTTTACCTTGGCTTTTTAGGTTTCGCCTTAATATTTCCTAGTTTTTACCTATCAATTTACTTAATTGGTTACATTCAATTAGGTTTAAAACTGCTTGTTGAAATAATGCAACAGTACAAACTTTATCCAATAGCTGCCATTGTGATAGAGCCGGCCAAAGTTTTATTCCTAAATAATGCCATTAACCATGGCGTGCTAACTCCATTAGGCTTACAACAAGTAAGAGACAGTGGTAAATCAATTCTTTTCTTATTGGAATCAAATCCTGGTCCAGGTTTGGGTCTCCTTGTTGCTTTCTTAATCTTTTTCTTTAAACGTGATAAAAAGCTTTCTAGTAATGCGGCATCTTCTGGTCCTATTCACCTCTTTGGTGGTATCCATGAAGTTTACTTTCCGTTTGTGCTATTAAAACCTGTGTTAATTCTGGCCACAATTGCTGGTGGTGTTGTGGGAAACGGCATTTTGCAAATATTTAATGCTGGGACAATTGCACCAGTGTCACCTGGTTCTGTTATTGCTGGTTTTCTCCAAATTAATAAAACACCTTTAGATGTAGCTGGTTATGCTTTGGCACTCGTTTTATCAGCTGTAACTTCGTTGTTAATTTCGCTTTTATTACTTTCTTTAACACGTAAAAAACAATTGAAAACACTGCAAGAAGCGCAAGCACAAGTTGCAGAAATGAAGCAAACGCCTGCAAAAAAACCAAGACAAAAAGATACTCCAGCAATTGCTACAAAGATAGACTTTTCCCAAGTTACCTTTGTTTGCGATGCAGGTATGGGTTCTTCTACTATGGGAGCAGCTATATTTAGAAAAGAACTAAAAAATCAAAATATTGAAGACATTACAGTGATTAATAAAGCAATAGTTGATTTAAAAGATGAAAAGGTAATTATTACCATTTCCCAACTTTATGATCGCGTAAAAGCGAAGCGTGCAGACGCTACGATTTATACCATCAATCAGTTCTTAGATAAACAAGGCTATTTAACTATTATTGAAAAGATTAAAAAATGAAAAGAATTAATGTATTGCACTTTGGCGCTGGTAATATAGGTAGAGGTGTTATATTACCGATTTACCAACAAAATGACTTTTCCATTGATTTAGTGGAATTGAATCAAAATACAGTTAATGAGTTACAAAAGCAAAAACAATACCAAGTTCATTACCTTGATTGTGATCAAAGTCAGTTAGTTAATGACTTTAATACTTGAAACTTAAAGGATGAAGCGAAGATTATTGAACTAATGGAAAGAGCTGATGTTATTTCCACTTCAATAGGTGCTAAAAACTTAGCTAGTTTAAAAACTTTGTTTGACAAAGCTAAGTTCCATAAAAGGGCTATAGTGCTTTGCTTTGAAAACGGCTTTCGGATTAGTTCTAACTTTAAAAACATTTTGCAATTAAATAACACACAAGTAAATTTTGTGGATGTGGTAATCGATACAATTGCACCTAACTTCGAAAAGAAAGCGAATTTCTTGGATATTTATTGCGAGAAGTATTCAGAAATTTATGCTGAAACTTTCCCGCTAGAAATTAAGGGAGTAAATCAAACGAATAGTTTAGATAGATTCATTATTAAAAAACTTTTATTAGTCAATGCACTGCACAGTGTTATTGGACTTTTAGGCTTTCAACAAAAGCTTAAGTATGTACATGAAACATTGCAAGTTAAAAGTAATTTGACTTTTGTAGAAAAATTAGCCCAACAAATTATTGATGCCCTTTGTGCTGAATATCCTGAATTTAATAAAAATAATTTGTTAAGTTATGGCAAAAATAACTTAGTCCGTTTTGCTAATCCCAAGATTCAAGATTTAAACACACGCTTAATTAGGGAACCATTACGTAAACTTAACCAAAATGAACGCTTTTATGCCATTTATAAACTCTTTAAAAAGAATAAGATTGCACTAAATAATATTTTGCAAGTTTATCTGATGGTTTTAAAAACTAATATAACAGATGATACCGAAAGCCAACAAATTGCTAAATTAATAAATGAAAAAGCTTGAACAGAGTTAGCGAAATTATCCAGTTTAGAAGAGTCAGAATGAAACCTAATTAAACAAGAATTAAGCCGTGAAATTACTAAAAAATAACATTTACATTAACGTTTATTTAAAAAACAAACAAGAAATTTTTGAATTTGTTTTTAAAAAGTTTAAAGAAGATGGTGCTGTACTAGATAGTTTTTTACCAGCAATTGTGGAACGCGATAAAGCAGCTAGTGTAGCAATTGGTAATTACCTCTTTTTACCCCACCCAGTTTATGACGAAATAGCAAACATTCAAAAAGAAAAAATGGTGTTTATTGGTTTAAAAGATGTAATTAACATTGATGGTCAACCAATTAAATTTATCTGTGGTTTAGCCTTAAAAGGTGAGCACCAAATGGATGCGTTGCAATCTTTAGCAATAGCTTTTTCAGACCCTGAAGAAGTGGAAAAGTTAGTTAAAGATAAGGATTTAACACAAGATAAGGTACTTGAATTTTTAGCAAAACACAACTAAGCAGTCACAATATTACTTAATTAAAAATAGTAAGTAACTAAATAAATTAACTTGCTTTTCGTCAGTTTTGTAACTAATATAGTCATTTAAAAAATTTTATCCAGATTTTTTTGGCTAAGTTCATCTTGTCCTTTTAATGATCAGCTAAGTGAAACTTTAAGCTAAATTCATTCTGTTACTATTTTTTCAGCTTTTAATTCTTCATTTTTAACGATGAAGTTTTCTGCTGTAAATTGTTTTTTGATTAGGTTTAATAGTAAGGGAAAGATCGAAAGAATACTCTTATCACCATTCTTGGCAGTGAAGCCATTATTGGAATACTGTCCATGCCAATTAAAGGTATAGATCAACTTAGCACTAATTGAAAAATTTTAATACTTCGATATTCAATGTCGAAACGCTCTACCTTTCGCTCCATTTTTAAAAGGATCTTTTTCTGTTGGATTAGTTACTTGGTCAAAATCAAGATCCGCAATGCCTAATTTGCCCTCTTCTGAGCTTTGTTTTCATGCGTCTAACAATAGCTTTGTTGTATCTTTTGGATTAACTTCATAAGATTTCTTTAAAGAATTTATGAGGTTTTGCCTACCTTCCGGAATTTTAATTGCTTTAGACAAATCAAAATCTTTGCCAGTTGTTTCTTTAAGAAGATTCCTAAGATTGGCTTGGATCCATGTGCAAGCACTTAATATTGTTCCACATAACAAAAACAAAGAACCGAAGGAAATCCTTGACAAAAGTTTCTTTTTACGCATTAACATAAAAAATAATGCATGATTTTGCTTTTTTGGAAAAGCCTTTTTAAGAAGAGGAAATTAATTGAAATTTCTGTTTAAACGTTATTTAAAAAATGAACTTTTTAAATTAGAAAAGCTATCTTTGTTGGTATTTTTATTCATCTAAAAAATACTCAAAAACTTTTTGCAAGTTTTGTTAGCACTTTTTCTCTACTCATGCAATTCATTAGTTAGAAGGGTGACAAGTGGTTTTTTTAGCTTGATCAGTGTGTTGCCTAACTTTATTTACTTAAATTACCAATGAGCAACAATAATAAATATTTCACTATAACTAAAAAACAGTACAAAAAAATGCGCCGCAATAAAATTGAACTTCTTTTTAATGTGAAAGTCCTAAAAAAGAAAAACGGTCGGCAAAAGTTCAAAATACTGCATGAAGTTGAGAATAAGCCAAAAATACCAATTAAAATAATTGAAGATCAACCGGAGTCGCCAAAGCCACCTAAAACTCCTAAACCCCCTAAACCGCCTAAAGGTCCTGACAATCCTGAGGAGCCGGATTCTCCAGAAGAACCCAAAGAAACAGATCAACCAGGTGGTCCCGATAATCCTAATGCAGGAAATAAAAAGATGCCAACACCTGAAGAATACGTAACTCGTAAAGAATTCAACGAATTCAAAGATTCAAACAATCAACGACTTACAAAGATTGAAAATAAGGTTGATAAGTTAGAAGTCAAAGTCGACAAATTAGCTGAAATTGTTCAAACTCAGGGCGAAGAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGTGAAACCCTACAGCTAATTTTGAAAACACTGCAAAAGATGAACGATCGTCTGGACAGAATGGAAACTCGCTTAGACAAGATCGATTCGCCCAAATAGCTTTAGGCTAAAAAGAAGATACCAATGGCTAAAACGCTGTTGGCATTTTTATTCATCTAAAACTGCCCAAAAAATTTTTTGCAATTTTGTTGGCACTTTTTAATTGCTATTTCAATTTATTTAGTGGAGGAGCAAAATGCAATTTTTTTGATTTTCTAGAATCCTCTAGAAAGGAGGTGTTAATTATGAAATATAAAAAGATAGGTGATATTAAATCTAAAGGATTTTTCGAATCTAAAGAAGAATATAAGGAACTTTTAAAAAAATGTGAACTAATTTCTTTTGAAGCTTTTTTCTGAAAAATTACCTAATGGTGATTATGAAGTGACTAAGAAACTTAAGCTTGTAGAAAAAGGTCCAAATTGAAAAAGAAAAACATACAAACAATCTTTAGAGAATGAAAAAATCTTAAAGTGGCTTATAGAGCACGACTAACAATAAAAGCAAGCCAAAATCGGCTTGCTTTTATTTTGTCTAATCTAACTCAAAACTCAGTTTACCGTACTTCCCGTTTATCAGTTCACTAACAAACAGTTCGCACGCTAAATTAATGTTGAGCTGATTCGATTTTTGCAACAACTTACGACTGATAGCAAATTTATGTAAAAACTCAGTGAAGTCTGTACCTTCATATGGTAACAATTGCGGATAATGTTTGCTTAAATAACAAAAAGCAAACGCACCTACATCTTCCAAAGGGACTACTTCCCTTTTAATAACGTTAGTTAAAACTAACTTGTAACCAACAGCCATTTCATCAATTCGTTTAAAGAAGACTCCTGGGGTATCGCTCAAATAAAACTCCGAACTTATTTGGTTTCAACTCATTGACTTGGTAACCCCAGCCCGATTAGCTACCTGTAAGTGATTTTTATTTAGTAACAAGTTAATTAAACTGGATTTACCAACGTTGGGCATTCCAATAACCGCTAACCGAAACTGGTGCACTAATAAACCTTTGGCTTTTAACTGATTCTTTTTTGCTTGAAACAAAGTTTGCAGTTTCTTAATAACTAAGCGTTTAAGTTGCAAACCTTGCTTTAAGGTTCCCCATAAAATATTGGAGTTAGCCACTGTTTGTGCTAAATCAGCCTTTAAAGCTAAAGTGAGTTTCGGTTTATTGGTAAAGTAAGCCGTAATTTCGGGGTTTTGGGTTAACGTTGGTGCACGCGCATCAACTACCTCAATTACACCATCTAAACTACTAGCTAGTTTTTTGAGTTGGTCATGGGTCTTCTTCATGTGACCCGGAAACCAGTTAATCTTGACTGCAGAGGTAAATGTATCCATCGATCCCTCGGATCCTTGTAATTGATACTAAACAATCTTTGTCAATTTTCCGGATGATGCGGATGAGGTTAGGAATTTCCACATACATAGTTACTGAAACTATCATCCGTTTTTTGGCTAATGAGTATCCACCCATCGTTTCTTGAATGGACAGACTGTGAGTCGCTTTATCGTTGAGCAGAGCCAACCTCACTTCTTCAATCTTGTCAGTAAAGACCTTAATTTCAGCAAAGTTATACCTTGGGAAAAGGTGACTCACTACTGTACCCGTAAATAACACCGAAAAGAAAGTGGCGATTAAGTTGGGTGAAAAGAAAAGGTTAACCTGTCAAGCACTTTTTTTGTAATCGGGAATATCTTGGAGCACAATACTGCCAGCGACAAACGAACCCATTAAGATCGCAATGATCAAAATAAAGGTATTGACATAAAAGAGAATTGGCCCCACGGAGCGGTTTTTCTTTCTCGCAAAGTATTGCGTTAGGAAATCGGCACCCCCAGCACAAGATCCCAAGATGTAGAGGATTGCTAAACTCGTACCGTTAACAAAACCATAGATCCCGGCATAAATAAAGGTCGAAATAATGACATTGCCTTGTGAAGTATCATTTCATAAGAACGGTACAAATAACGCGCTTTGATCAACACCTTCAGCTTTTTTTGCAGCTTCCCAAAATTCAGTGTGGTGCACTGCGGACAACATCGATGGCAATTGATTAGCCCCCGGAATAATACTAAAGATAAAACCAAAGACGTTGGAAGCGACCACGTAATGGGTTGACAGAATGGTGAAACGTTTCCCAATTTTTTTGTAAGAAAAAATAATCAGGGGAATGTTGACTATTACATAAAACAGCCAGTAAAAGATGTTGAACACTAGGTTGCGTTGTCCATCTTCGAGGTTCTTCAACAAGACAAACAGTAAACGCGCAAAACCTTGGGTTAGCGAACTAATCCCAAAGCTGTACAATCCCGTTTGTTGGATAAACACCAGCAAAAAGAAGGCGTTCACTAAAGCCAGGATGTAGACAATTGTGTACTTTAAAGGTTTTTTAGCCTCATAAAGGGCACTAAACATCAACAGCGAACTAGTAATCTTGGCCCTTTTAGAAGCTGAAGTAGTTTCAGTTTTGTCTTGATCCTTCCTTGTGAATCACCTTTTCAGTCAATCCATGGTGAAAAAGTAAAACTATTGTTTTCGCTCTTTAATTTTGGCAGCTTTACCAGAGCGCTCACGCATATATGAAATGTAAGCGCGTCTTACTTTACCCCGTCTTTTCAATTCAATTGAAATATTAGGGTTATGAATTTGGAAGTTTTTTTCAATCGGAATACCATCAGTGGTTTTACGCACAATGAAGGTTTCACTAATACCTTTACCACGACGGCGTAGGACAGTTCCCGTAAAGTTTTGGATTCTGACCTTTTCCTTTTCTTTAAGTTTGATGGCAACATTCACTTCGTCACCAGCGCTAAACTCAGGCACATAAGCCTTTAATTGCTTTTGTTCTACTAAATCTATTAAAGCTTGTTTATTTATTTTTTTCATCTTTCTTTGCTAAATATTGCTTATACAAATCAGGACGGTATTGAGCCGTTCTGAGCAGTTGTTGTTCTTTGCGATAAGCCTCTATCTTTTTATGGTCACCTGATAATAATATTTCAGGTACCTTATCCCCGTCTAGATCATATGGCCTAGTGTAAGCGGGGAAATCCAGTAAGTAGTTGTTAAATGATTCGTGGATTAAGCTCTGATCATTAATGACACCCTTAATTAAACGAACAGTGGCATCAATCACACTTAAAGCCACTAACTCCCCACCACTCAACACAAAGTCACCTAACGAAATAATTTGATCGACATGTTTATACAAGCGGTAATCAAAGCCCTCATAGTGACCACAGATCAAAATGAGGTGTTCCGTTTGGGTTAAAGCTTGCGCCATGGGTTGGGAAAACTGTTGTCCCTCAGGTGATAATAAAACTACTTTCGATTGTGGAGTTCTACAAGCCTTTAAAGCTTTGAGAATTGGTTCAGCTTTTAGAATCATCCCGCCACCTCCTCCGTAAGCCATATCATCAACCGTTTGGTGTTTGTCCTTGCAATACTGCCTTCAGTTAATTACTTCAAACTCAACGAGCTTTGCTTTTTGAGCTTGGGCCATAATCGAAGCGTTTAAGTAAGGTCAAACTACCTGTTCAAAGAGGGTTAAAACAGTGATTTTCACACTTACTTGGACTTAGCTACTGGCTTTTTCTTACTTTCTACAAACTTTTTCCAAAGACCAGTCTGACTAAACAAAGAGCGAACTGTATCAGTTGGTTTTGCTCCCTTATTGAGTCATTCCAAGGCCACAGCTTCATCAATCTTGCACTTATTTTCTTTTAATGCAGGATTGAGATGGCCAATTAACGCAATGTACTTACCATCACGTTTAACTCTGGAATCAACAGCTACTATTCTGTAGGTTGGGTAGTGAACACGACCCATGCGCATTAATCTTATTTTTACCAATCAAGTCTCCTTTTAAAATAAAGCGGATAATTATACATTGCCGTTAGGTAGTTTTACCTAACTCTAAGAATAGCGCTCAATGAACTGGACAATCCGACTCTTATGTTTTTGAGCGAAGTATTTATCCATAAAGAAGTGGATGTGTACTTGGCCAATCAAAACGTTAATCAATCCAGCCAGAGCAGCGTTAGCCGTTAATAACCAGATAAAGATTTCAATGTTTTTTGTTTGTAAGGTAATTGCTTGAAAGATAAACAACAAGGGTACAAAAACAATAACCCCTTGCATTAAGGAAACTATTCACGAATACAACACCCGGTTGGTATTTTGGAAGTACTGTTGACTGGTAGCGCCAATGGCCACAAAGAACACTTGCGCTTGCACAATTAAGGAAAACTGATTAGCGAGCATTAAGGTAGCTGCACTTGTATCAAACAGGTGCACTAGGATTTCTTTGCCCAATCCAACTGCCAGAATTAAATACAGCAAAGCAGCAAATACCAAACACACTAACAACGTATAAACATTAATCCTTTTAATGTCAGCCAACTGGCCCTGACCAAACTTGTAGGATACCACTGTCCGCACCCCTTGGAGAACACCAAAGATGGCGGCTGAAGTTAGGTTAGCAATTGCAATGGGTCCCGTTAGTAACACCAAGTAAAAGGTATCACTTTGGTCCGTAAGCGTTTTGGTCAAATTCACCAAAAAGCTTTCGTAAAAGGTGTTGAGAATTGAGAGCGAACCATTACGGAAAAAGGAGGCTAACCCCACCATTGAAATGACCAAAAAGAGGTTAAAACTAACCCGTTTAAAGGAAAAGTCCCTCAACGAAAGGTAGGTTAAACCACGTTTATTTAAACTAATAAGGTACACCACATAAGCCATAAAGGTAATGAAGTAAACCAAAATCGCAGCCACCGCGGAGCCCACAACCCCTAAAGTGGAAAAACGCACTAACAAGAAAACAAACAGGACGTTCAGTAAGTTCGATAAAGGAGGCACAATTGCAATAAACAGTTGCCGGCCCTCTGATTGGCCTAAGTAAAAAAACAAGCGGCTCAACATCGGTATGATGTTAAAGCCAATCAAAATGTAAACGTACTCTGAACCAATCGCAACCGCCTTTTCGTTAAAGAAGTGTTGAAACTGATCCGCTACTTGCACGTGGTGTTGTTCATCGACTTTATCCAAGTTAAACTGCAACCACTCTTTGGCAATCGCTAGCACAATAATTTGGGTCACTAGGGCAAATAAGGTAGTGGAAACTAACCCGGTATTCCAAGCATCCTTAATCTTTTTCTCATCACCCTTACCAATGGTTTTGGAAAAGATCACCCCCGTACCCAACGGCACAAAGATGGTAATGGCGTTGAGAATGACTACAATTGGCTGGCTAATATTTAAGACTGTTTTGACTAATTGACTAGCTGTTAGTTCCGTGTGGTTGGGAATGTCCCCACCCTTATAAAACGCGGATGCTTTAAATTCCTCAATTAAGGCCTGGTCAGTAAAGATTGAATCGGGATTAAGTGGTCCATGCGGGACAAACTTAATCACCATAATTTGGTCCACAAAGACATAAGCAGCGGAAAACAGCGCAAAGAAAAAAGTCGGAATAGCAAAGCGCAAGATCGTTAGAAACAGTCTTTTCGATTCAAAGGATTTTTGGATGAGGACTTGCTTCGCTAGACGATCTTGTTGGCTATTCACTTAGTTAGATTCCTTAAATAATTTGAGGTATTCACCATACCCCGCCGATTCCATTTCCGCAAACGGAATGAACTTTAGAGCAGCGGAGTTAATGCAGTAACGCAAACCACCATGCTCTTCGGGACCATCACGAAAGACGTGCCCTAGGTGGCTGTTGGAGTTTTTGGCGCGTACTTCGGTGCGGATCATCCCGTGTGATTCATCGCGGTAGTTAGCAATTAAGTCTTGACTAATTGGTTTGGTAAACGCTGGTCAACCACAACCGGATTTGAACTTATCAGTTGAGATAAACAACGGTTCACCGGACGTAATGTCAACGTAAATACCCTTTTCGTAATGGCGGTTATATTCATTAGTATAGGGCGGTTCGGTGTGTGCGTTTTGGGTAACGTCGTACTGTAGTTTAGTTAAGGTCCGCTTTAGTTCCCCGTCACTCTTTTTTTGGTACTTACTCATGCACTGAATTTTTCACTATTATTTAAGTAAACATCAAGGAAACTTTTGCCTATTTTGATGTGCGACTTAATTTCAATTATACCCTGATTTTTAGGTTTTAAATGGAGCTCTTTTTCCGAACAGAGCATTCCCATGGAATCATACCCCGCAATTTTAGCCTTCTTGATAATCGTGCCATCAGGTAAAACACCACCAACATGGACTAAAACAGTTTTAAGGCCAACCCGAACATTATCCGCCCCACACACGACATCCAAGGACTTATTGGAACCAGTGTTGACCTTACACCTTTTCAAATGAGTGTTGGGAATCGGAATGGCCGAAATGACCTCACAAACCACAAAGGGTACCTTTTTGGCTAAGGGTGTTAAATCATAGTTCAACAGCTCACTAATCTTTTTGACCAACTTCAAAGAAGGGTAATTCAACCCTGCGGTAAAGTGATGGGAAATTAATCCCCTGCGAAAGCTCTTTTTAATGTCAAAGAAGTTAAACCCAGCAATCTCATTGGCTTCATTGACAAAGAAGCTAAAGTTCTGGTTCTTTTCTTGGCGCAAAGTAGCTTGCGTGCGGCTACCAATTAAACCAAACATACAGTTCTTAAGTGTTTTCTTGGGACAAAAAATGGTGATAAAGTCCTGAGAAATATCAAAAAAACCGTAACGCATTGTTAACTATTCGCCTTGCAGCACAATTACATAGCTGTTCAATCCCGTGTGGGTACAAATGACCGGGGACAGTAAGCTCTGTTCTATATTAATGGAATGATCCAACAGTTTATCAAACTCTTGGACTAAATTAGTGTTGGTCTGTTCGTCAAAGACGGTGGTAAGAAAGGCACCCCGTTTCAGTTTAGCGGAGTGTTTTAACAGCTGTTTCTTTAAAAACTTGACTGCTAGCTTATGCGCATCATGAGTCGAACGCGCTTTATCAAAGAACTGTAACTTACCATTTTCACCTAAAAAACTAATCAGTAAGTGAAACTTTAAGCTCTTGATAATAAAGGATTTAAGATTGGAAAGCCGCCCGCCTGCCACTAGTGGTTTGGTATCAGTGACAAACAACACTGCGGCCGTTTTCTTGCGGTACTGCTCCACAAAGCGATCCAAGCCTTCCCTGGAATAAGGATTGGTATTCAACCAACCCTTAATGTCATTGATTAGCCAATCAATGATCATTTCCACCGCTCTGGCATCCAATACATGGAACTTCGAAGCGTAATCCTTTTCAACTAACTTCCAGTTCGCGTACGAAGCGGAGATTTCCTTATCAATCGGAATGCCGATAATCAGGTCATACTGCTCCACTAGTTCACTCACCTTAGCGCGCACTATTTCTTCAGTAGTTTGCGATGTGGAGAACTTCACTCCCTTCGGAGCGTTAGCCATCTTGTCCGTGAGGGTTTTTAAATCAATGTCAAACCCCGAACTAAAGGTGGTTTCAGCTTCAGGAGTTGATTCAATGATGAAAAGCGGTAGCACATACAGATGTTGTGCCTGGCTTTCCTTGAGGTTACTAACAGAGTCAACTAAGAATGCGACTCTCACTATTCAAGCACTTCCGTGACTGAACCAGCACCAACCGTTCGACCACCTTCACGGATGGAGAACTTACTACCTTTTTCACAAGCAATTGGTGCAATTAGTTCAACTGTAATCGAGGTATTGTCACCTGGTAGCACCATTTCGGTGTTTTCTGGTAGGGAAATCGAACCAGTAACGTCTGTAGTACGGAAGTAGAATTGGGGACGGTAACCGTTTAAGAAACCGGTGTGACGACCACCTTCTTCCTTCTTTAAAGCATAGATTTCCGCTTTAAATTTCTTGTGCGGTTTAATCGAACCTGGTTTAGCTAACACTTGACCACGTTCCACTTCTTTACGGTCCACACCACGGAGTAATACCCCAGCGTTGTCCCCAGCCATTGCTGAATCAAGTTCCTTTTTGAACATTTCGATTCCGGTAACAACTGCTTTACGGATTGGACGTAAACCAACGATTTCAATTTCTTGACCTACTTTCAATTCACCACGTTCAACCCGACCGGTAACCACGGTACCACGGCCAGTAATCGTCATGGTGTCTTCGATTGCCAACAAGAAGGGTTTGTCCACTTCACGTTCAGGAGTTGGAATCCATTCATCAACTGCATTCATTAAATCATGGATCTTAGCTTCCCACTTAGGATCACCTTCAAGCGCTTTAAGTGCAGAACCATAAATAATAGGGGTGTTCTTGCCATCAAAGCCGTAAGAAGTTAATAAGTCACGTACCTCTTCTGCTACTAACTCTTGCACTTCTTCATCAGTTGCAATGTCACACTTGTTTAGGAACACTACCATGCGTGGCACACCCACTTGGCGGGCCAACAAAATGTGTTCACGGGTTTGGGGCATAACACTGTCAGTTGCTGAAACTACTAGAATGGCACCATCCATTTGTGCAGCACCAGTAATCATGTTCTTAATGTAGTCAGCGTGTCCTGGACAGTCAACGTGAGCATAGTGACGCTTGTCAGAGGAGTACTCCACGTGAGCGGAGTTAATCGTAATTCCCCGTGCTTTTTCTTCCGGAGCCTTATCGATTTGGTCGTAACGAGTAGCAGCTGATTTACCTTCTTTTGCTAATACAGTACAAATAGCTGCTGTTAAAGTTGTTTTACCGTGGTCAATGTGGCCAATAGTACCTACATTAACGTGGGGTTTAGATCGGTCAAATTTCTCTCTTGCCATGTGTTTGAATTACGTCTCTAATTTTACATAAGTTTGAGATAAAGCTGTAGAGAGGCTTATAATTCTTGGAGTAGTGTAATTCATATGGAGCAAAATAATTGCAAAATCAGAACTTGATTTGCCAAACTGGCGTTAGCACAAAAGGTCTTCTTGGGGGTAATTCCCTTATTTTTTATTTGTTTTGTTTTTGTAATTGCTGACATTGTCATCAGTTTGCAAAATAAAGGCCACATTATAGAAGAGATAGATAAGTTTACTAACCAGAGCAATGTTATGTTGCTCATTTATGCATGTTGGTATGTAAGCAAACCAAAGAGCCATTATCTCAAGAACCAGCAGTTCTTTTTGAGTGCCTTTGCTTACATCATCTTTACTTTTTTAGGCTACAACGTCATTTTGGCGGCTTCACAACAAGCTTACAGTGATAAGGATGCTTATTCCTTGGCAAGTTCTGTCTTTCTCCATGTGTTGGCACCAATTGCTTTCTTGGTTGCAGGTATAGTTAAGATGAAAACGGACAAGGATGTAACCTTTAATCATTTTTGAAAAAGTTTGGGTTACTTCATGATCTATCCCTTAGTGTATGGGTTGTACTTAGCGACCATTCCTTATGTAAGGGGTCATTATGTGAGTGATGATGGTAAGTCTACTACCTATGTGGTTTATGGGGAGATTACCAACACCAAGGATAACCCGATCGTCGCTTGACCAGTAGTAATTTGTTTCCTTTTCATTTACTTTCCCTTGAGCTTCTTAGCGGTTTATGCCTTGCAATGTAAGTTATTGAATAGACCTCTAAAAGCACAATTTAAGTGTGCAACCAATAAATGCCCAAAATAAGAAAAGCCGTTATTCCCGCAGCCGGACTGGGTACCAGATTACTCCCAGCCACTAAGGCCATTCCCAAAGAGATGTTGCCCTTAGTCAATAAACCCACAATTCAGTACATTGTGGAAGAAGCAGTAGCGAGTGGCATTAAGGAAATCCTAGTAATTGTGTCTTCTAAAAAGGAAGCGATTATTGACCACTTTGACTATGACTTTATCTTGGAAAATGCTTTACTCCAAAAGCACAAAGATCAAGAACACCAAGAAATTAAGGATATTGCCAACTTAGCGCACATTTACTTTGTCCGCCAAAAACACCAACACGGTTTAGGAGATGCCATTTTGCACGCCAAGTCGTTTGTTGGTAACGAAGACTTTGCCGTCCTTTTAGGTGATGATGTCGTGTTTGGTGAACAACCGGCATTAGCACAATGTATTCAAGCTTATGAACAAACAGATTGTCAGGTAATAGGCGTGCAAGAAGTGCCCCATGACCAGGTCAACAAGTATGGCATAGTAACCCCTGAAGCTAATTGACAAAAACAAGCGTTAGTCAAAATTTTGGGCATGGTGGAAAAACCAGCTGTCAACGAAGCCAAGAGCAACCTAGCCATTTTGAGCCGTTACATCCTTAAACCCAGTATTTTTACAGCTCTTAAACAAGTACCGTTTGGTGTTGGAGGTGAGTTACAGTTGACTGATGGGTTGAACTATTGTTTACAGCAAGGGGAACCCTTCTTTGCCAAGCACTTTGGTGGTACCCGGTTTGATGTCGGTACTAAAAATGGTTTTATTAAAGCGAACCTGTACACGGCTTTAAAAACCGATGCCATTACTAAGGATGAAGTCTTGGCCATCCTCAAGGAATTTGTTTAAACAATTTGCTTAAAGCTTGGCACCGTTCAAGGTTAAACCCAAGAAGTTTTCTGGTTTAATTAAACGGCTAAACGGACACATGGCATGCGCTGCCGTAATGACTGTTTGCGCTGTGGTTTGGTCCACGCCCGTAATCGCTAATTCAACACCGGCTTTAATGTGGAACAGACCGTCCTGGTCATGCAGTTCGACACTAACACCCACAATAGGTTGAGTAGCTAATTGCAGTTGGTGCTGTTGTAAAACCACACGCACAGCTTGCGAAAAACAACCAGCATAAGCTGAAGCAAACAGCTGTTCGGGGTTGTTTTTGTCCTGGTGAGTAGCACCAGGTTTAGGAAATGCTAAACTAACGGTAAAACCATCAACGGTTTGGACAACGCCTTCCCTGCCGGCGCTAGCGTGTGCTGTTGTTTTGTAAATTACTGCCATTGTTACTGATATTATATTTAGAAAGATGACAAAAGATCTCCTAGCACTTTTAAAAGAACGGGGCTTATTTGTACAAGCTAACTTTGAAAAGGAATTAAAGCAATTATTAAACCAAGGATCATTTGCTTTCTATGTTGGCTTTGACCCCACAGCACCCTCACTCCATATTGGCAACTATGTGCTGCTCCATGTAGCACAAATCTTCCAAGCAATGGGTCATATTCCCCACGTCTTGTTGGGTAGTGGGACGGCTTTAATAGGTGATCCTACTGGCAGAATGGAACTGCGCCAGATGATGAGTCGTGAAACAATTGCCGAAAATACGCGCAACATTAAAAAACAAATTAGGCGCTTTTTGGGTAGTAATGTAGTGTTTTGCCAAAACGAGACCTGGTTAAAGAAACTCAATTACATTGAGGTCATCAGGGAATTGGGACCCTGCTTTTCGGTCAACAAGATGTTAGCGACCGATGCCTTTAGTGCTCGTTGGGAACGCGGTTTAACGTTGATGGAATTGAACTACATGGTATTACAGGCCTATGACTTCTATTATTTAAACCAAAAGTATGGGGTGCAACTGCAAATAGGGGGCAGTGACCAGTGGGCTAATATCCTAGCGGGGGCTGATTTAATTAGACGCAAAACCCAAAAACAGGTGTACGGCATGACCACTAACTTATTAGTGAAAGCTAATGGGGAAAAGATGGGTAAGAGTGCTAGTGGAGCTTTATGGTTAGATCCACAAAAGACCAGTCCGTATGACTTCTACCAGTATTGGATTAACCTTGATGATGCTAGTTTGCAAAAGGTTTTCCTTATGCTCACTAAATTAGATACCAAGGCAATTGAAACCCTCTGCAACTTGAAGGGCGCTGCCATTAAGGAGGCCAAGGCAAAACTAGCCTTCGAGTTAACTGACGCTATTCATGGTACCAAGGCAGCTTTAGCCGCCCAAGCGAAAAGTGCCCGAATCTTTGCCTTTCAACCTGATACTGAAACCAAAACCGTGCGAGCTGGCACCAGGTTAGTGGATGTGATTGTTGATTTAGGTTTAGTTGTATCCAGATCAGAAGCGCGCCGGGTGATTCAGCAAGGTGGTTTAACGATTAACCAAGAAAAAGTGACTGATGTGGAAATGGTCCTACAAGCGAGTTCCCAACCACTAGTGATTGGTAAGGGCAAAAAGCGTTTTGTAACGGTGCAAGTGATAGCTAACACCAAATAAAAAACCATCTTTGAGAGTGGTCTTTTAATGAACTTAGTGTTATTTCTTAGCCTTCTTCGCTTTAACTGGTTTGCGCTTGGAATTGGTTGTGGGTTCAGTAACTTCAGCCACAACTGTTGTTTCTGTTGCACTGGTAGTTTCAGCGTTGTTGAAATCACCAATGAGTTTGGTCTTTAACAGTTCCACTACCTTATGGAATGAATCTAAGGTAGCAGCGTCATAACCAGCTTGACGGGTAATATTGAGTTCTAAATCACCCACAGCTTCCTCCATTCTTAAAGAAGTATCCATGAAGGTAATCGGTACACTCATCATGAGGTAAGTGTGTGTGGTATTGTTGTAAACGTTAAAGTTAGCATACACATTAACCGGGTTAAACAACACCTGCTTTATCATCTCCTTATCTAACTGATTAAAGTTAAAACTAGCGTCTTTTTCAGTGTAGAGCAAAACGTTGTTCTGGCGGTTACTTTCAGTTAAAGATTCGTAAAAACCAGTGTTAATTAAGGGGAAATCGCTTTTAGTTAAGAACCAGTGTTGGTCACTAACAGCATCAATAATAGCGACTAAGCAAGCCACCTTTTCATTTTTGGAAGTACTTCTCTTAATGGCACTTAACTTAGCAAAGTTAAGCGTTTGACCATTCTTGTACTGTACCTTAAATTGCTTGGGAACTAACTCAAAGTTATAGATGGTAGCAGGGTAAAAAGTCGGTAGAATATCCGCACTAATTGGACCTTTTTCAAACAATTCAAATTGCTCAAACTGCTTGTTGTTTGCCATCTTTTGTAACCACAAATCAGTACGATCTTGACGGTTGACCAAATGAATTAACTGACTGCGTTTGTACAAAGCTACAAAGTTAATCACAAAGCTCAACAGCAAGGTAAACCCGCCTAAGGCAATTAGGAAAAGAGCTGCCACTTTAGGAACAATTGAACCCTGACCAACATAGCTGTAAATCCCTAAAGAAAGGGCAATTAAAGCAAAGACACCACAAAAACCAACAACTCTTGTAAATTTATTGAACTTTTCAGGTGTTAAAAAGAACTTTTGAAAACGAGTTAGCTCTGGCATGCGTATATAAAAGATTGTAAGAAAAGATAAGTTAAACACAACGCTGGAGTTGTTTGCCCTACAGAACGTTTCCCTAAAAACTGCGCCTTGCCAAACTGCGCCCGGGTTTGACAAAGCCGTTCATACAGTTCAACTAACACCCCATCGAGCTGCTTGTACTGCCTTTTGGTGCGGTTTACTAATTGCAAGAAGCTTTTACAAAAGTAATACAACAAATCCCACACCCGTTTGTGACCTAAAGTAATTGCTTTAGTTTGTATCAATTTTTCGGTGTGTTTAACAAGGGTTTTCACCACGGTAATCACATCCTTGTTTTCTTCTAACAACAGGTATTCTACAGCCTCCCACCAAGTTAGCACAGCATTGGTTTCACAACCCAACAAGTTGGAGTGTTGCTTTAAGACTGCAATAAAGTTTTGCGGGGTTTTACCAACATCGTTGAGGGTGGTAAAACACTGAAAGCTATAGGTGCCCAAATCGGGTTGCAAGTGGTTGAGCTGCTTTTGCAAGTGCGCGAGGTTAACTAGGAGCTGATCGACAAAACTAGCAAACTTAACTGTTTGTTTCACTGTCTTTTTCTGTATCCTTCTCAGTCTTCTTATTTAACTTAGCTTCCTTGGCTTCTGCCTTTTGTTTGGCTTGCTGTTCTTGCTTTTGCGCTAGGATCTCTGGAGGGAGTTTAGTGTGTTCGTGAATGTAATCGATGTCACTCTTTAAGATGGTTTCGGCAATCAACAAGGCTTCAACTAACAATTCCAACTCCTTACGGTTCGTTTTAATAATGGTACGTGCCTTCTTGTACTGTTCTTCAATAATGGCGTTAATCTCAAAGTCAATATCCTTTGCTGTTTGCTCAGAGAACAGTTTGGTACCTGGTGGGACGGTACCTTGACTAGGTACATATTGCACTTGCCCTAACTTGGACATTCCTAACTGGGTAACCATGGCACGGGCAATATTGGTCGCCTTATAAAAGTCCGAGGAAGCGCCAGTGGTAATTTCTAGGGGACCGTAAATTTCTTCCTCTGCAGCACGGCCGCCCATGGCTGTAGCAATCATCGCTAACAAGTCAGATTTACGCTTTAAATTGAGATCGCCACTCTTAGGGGTTGACAAGGTATAACCACCCGCTTGACCCCGGGGAATAATGGTAATCTTTTGCACTTCATCGTTACTGTGAACATGGAGGCCAACCAAAGCATGACCTGCTTCGTGGTAGGCCACTAGTTTACGATCAGCATCACTGACCACCCGAGATTTTTTGGCAGGACCAGCTATGACCCGGTCAATGGCTTCATCAATGTCGTTCATGTTGATCGTGGTCCGGTTATCACGGACTGCTAACAGGGTAGCTTCATTAATGACGTTTTCCAATTGAGCCCCACTAAAACCAGGGGTACGTTTAGCAACATCCAATAAGCTAATCTTGGAAGACAAGTTCTTGTTCTTGGCGTGCACTTGTAAGATGCCTTCGCGTTCCTTAATGTCGGGAAGGTTAATCTGGATGTGACGATCAAACCGACCTGGGCGTAATAACGCATCATCAAGGACATCCAAGCGGTTGGTAGCAGCCATCACCACAACACCAGTGCGGCTAGTAAACCCATCCATTTCCGCTAACAGTTGGTTTAAGGTTTGTTCGACCACCGAGTAGGAGGACAACTCCACGCGACCCCGTTTAGAACCAACCGAGTCAATTTCATCGATGAAGATAATACATGGGGCCGCTTTCTTAGCCTTATTGAACAAGTCCCGTACCCGTTTGGCGCCCACACCAACCAGCATATCTTCAAAGCCAGAACCCGTGGACTGGAAGAAAGGCACACCAGCTTCACCAGCAACAGCTTTCGCTAACAGCGTTTTACCCGTACCAGGAGGGCCGTAAAGAATAACACCGCGTGGGGAACGAGCGCCCATTTGGGCATACTTCAAGGGGTTTTTGAGGTAATCAACAATTTCCAGTAACTCGTGTTTTTCTTCTTGGAGTCCCGCAATGTTGTCAAACCTCACCGAAGACTTTGCGAGTTTAGCTTGGGTCTTACCAATTGAGAAGATGTTGTCCTCCTCACTGCGACCACCACCAGAGATACCGCGAGCAGAACGTCAAAAGAGTAAGAAGAAAATGACAAAGATAATAATTGGTAGTAGGCCAAAGAAGATGTTGAGCACATCACGTGCTCTCGTATCAGGAGCAACAAAGGTACCGATGTCATGGAGACCTTTAATAGAAGCTAATTCAGTGCCATTGTTACTTTTTTGGTCTTGGGTCATCATTCCATTAACTATGGCCTTTCCAGTTGACTGATTAATCGAGAGGTTTGCTATATTAAAGATAGTTTCCCCACTACCATTAGTAGCTTTGTGGGCAGTTACTACTATGGGATTTATCCCTTGGAAACTAATGGAAACCTGCAGAATGTCATCGGTGACATAGGTGGAATTATTAATTCTTTGGAAGGTGTGTTTGCCTGATAAGCCTTTGATTTGAGCCGAAAGTGTAGTTCCACTAAGCTCCCACTTTTCAATTACAGCCGTGGTAGCCCTTGGCATTAGGATATAAACCAAAATACCAATAATAATGGCTAGGATAACCACTCACCAAAAAATCTTTCACGCTGTGCGTTTGGGAAACTGCGGTTTTTCGCTCGTAGTCGCCTCGTTAAGTCCTTTATTTTTTTTCATTTGTTGTTGTTTGTTGGGTGATTTGACTGGTTTTTATTCTAACAACACTAAGGGTAACTAATACACCCACTGCTGCAAAACCAGCCAAAGTTAGAAAGGTAATTAAGATGATTTTTTTGTGCTTTTTTACCAGGGAAACCAACACACTAAACCATCGCATAAAATTCTAAATAGCGCGACTTAATTATCTTCAGGATCAAAGACACCAACATAGGGTAAATTGCGATAAAAGCCCTGGTAGTCTAACCCAAAACCAACCAAGAATTCGTCTTGGTTTTTAAAGCAAGAATAGTCCACCTTAATGTCAGCTTCCCTGGGTTTGAGCTTTTCAATGAAGCTCACTAAGATCACACTTTTGGCCTTTCGGGTGTGGAGTAAGTCCAATACTAATTTAATAGAACGACCACTGTCGATTATGTCTTCTATTAAAAGAATGTCCTTGCCTTTAGGGTCATGGGACATATCTAGGACAATCTTCGGTGCTTCTTGTTGGCGACTGCCACCATGGTAAGAAGCGACTGCCACAAAGTCCAGTTGTAAATCAAAGGTAAACTGACTAATAAGTTTGCCCAAAAAGGGAATACAACCCTTCAAGATCCCTAATACTATGACCTGTTTACCAGCAAAATGTTCATTACACCATTTGAGCGCAGCGTTACAACCCGCTTCAACCTGTTTTTGATCAATGATGATAGATTTAATGCCCATTACTTTAATCTTTGTCGTTCCACTAACACGACCGCTAAACAACTAATTTCATTCGAATTCAACGGTTCACGTACCTTGAGGTTAATTGTCAGTTCGGTAATCCGTAAAGCCGTACAAATAAAGTCAAAGATGGCATGCTTATAGTCATCCATCTTTTGTTGTGAGGGAATTTCTAAGGTGATATCAATGTTATTGATGTACCAGTTCTTGCGAATAAAGTTGTAAGTCCGTGCCAAAATACGGGGCGCTTCTTTACGCGGATTACCCTTTAAAGGGATCTGGGTTTTTTCTTTATTGAAGACAATTTGTCCATCACCTAAGGCAGTAGCACCAAATAAAGCATCAGCTACTGCTAGCTGGATAACGTCACTGGCGTCATCACTAGCATCGTGGTCATAAATGTACTTGGAATCTTCTATTTCCACCCCACCTATTCAAAATTTATTCTTCTGTTCTGCTCTCGGTTTAATGCGGTATTTTTTCTTACCTAGGCCAACTCTAAACTGCATCGTGGTATCTGTTAATTATACTTTTGCCCACCCCCTTTTCCTTTTATGTTGAGAGCTAAAAAAGACAAAGCTTGGCAGCAGGTTTTATTTAGATCAAATCTCAATTGGGAACAGTAAATGATTGGTGGCTTAAACCAAATTAAAATGAAGGCAAGCTTGAATAAATTAGTTATGAAGAGTCTTAAAGTAGCACTCATTGGTTCTGGTGCAGTGGGCACCAGCTTTCTCTACGCTGCTATGAGCCGTGGTTTAGCGAGTGAATACATGGTTATCGACATTAACGAAAAATCCCAAGTAGGGAATGTTTTTGACCTCCAAGATGCTGTACCATCATCACCGCAATACAGTAAGGTAATTGCTGGTGATTACAAACAACTCAAAGATTACGACTTTATCTTTATTGGTGCTGGTCGACCCCAAAAGCAAGGTGGGGAAACCCGGTTACAACTCCTTGAAGGTAATGTCGAAATTATGAAGAACATTGCCAAAGCCGTGAAGGAATCGGGCTTTAAGGGCATTACCTTAATTGCTTCCAACCCAGTCGACATTATGGCTTACACCTATTTAAAGGTAACGGGGTTTGAACCGAATAAGGTAATTGGTTCTGGAACCTTACTAGACAGCGCCCGGTTAAAGTTTGCGATTGCTGAAAAATACGGTATGAGCAGCAGGGATGTCCAAGCATATGTGTTAGGTGAACACGGGGATAGTTCAGTAAGTATTATTTCCTCCGCTAAGATTGCTGGCTTACCACTCAAACACTTCTCGAAAGCAAGTGATATAGAAAAAGAATTTGCTGAAATTGACCACTTTATTCGCCGTCGAGCTTATGAAATCATCGAACGCAAAGGAGCCACCTTTTATGGCATCGGGGAAGCAACTGCCGAAGTAGCAGAATTAATTCTCCGCGACACTAAAGAAGTACGAGTAGTAGCATCGTTAATTAATGGCCAATATGGTGCTAAAGATGTAATGTTTGGCACCCCTTGTGTCTTGGGACGCAATGGTGTGGAAAAGATCTTGGAAATTGAATTGTCTGCTACAGAAAAAGCAGGCTTGGACAAATCAATCCAAGTATTGAAGGACAACATTAAGTTAGCTAAACTATAAGAATTAGTTTAACTGTAGACATAAATAAACTAGTTAACTACTAGAACAAAACAAGCATGCTGACCCATTGGGGTTGATGTGCTTGTTTTGTTTATTAAAAAATAGGCAAACTTTTTGGCGTATTTGTCAAATTCATTCAATTTATTAAGTGGGAGCAGGAAGACCGACTTTTGTTGAAAGTTTTTGCTGCTCCCGGCCAACTAGAAAAATATTTAAATTCATACATCTTAGTGTTTACAACTCAAACTAATATTCTTCGTTAGTTTTCAATATCGTTAAAAAATATTTTTTAGCCGTCTGCTTGTCAACTTGAGCAGATGGTTTTTTATTTATTTGAAAAGAATAAAAATACCAGCAACTCAACCTTAAAGATTAAATGCTGGTATTTTTAGTTTGAGTTGGAATTATTTAGATTCCAATTTGTCTATACGTTTGCCAAAGTCTATTAAGGTATTGTTAATTGATTCCAGGCTTTTAAGCACAAGACTTTCAAAGCGATCCATGCGGGCGTTCATTGCTTCACCCTGAATTCGAAAGCCTTCTCTAACTTCTATTTGGAATTCTTTAAATTCTGCCTTAGTAACATATCTTTGATCAAACTCAACTAAAAGCTCTTGTTTAAAAACTTCTAGTTTTTTGTCCAATTGTTTATGGGTTACGTATCCGCTTTTGCCTTTTTCCATATGGTTTAAATTGCCATAAAAGCCCATGTTATAGCTACCATCAGATAGATCTTCGATTCAGGCCTTTGCTGTTTTAATCTTATCTTTTTTCAAATTTCTTTTGAACAAAGTAATTGAAATTTCGCGTTTAATGGTCAAAACCTCCTTTCTTTACAGGATTTAAAAAAACAGAAAGCTTTATATGAGTGTTGGGCCAAATTTAAAGAGGGTCCAATTTTTCAAAAAATGTTAATCATTTCTTTTAAGCTCCTTTACTAAATAAATGGAAGCGGTTATTTAGAAGTACCAAGAAAATGAATTTTTAAATAGAAATCTGTAAAGAATGAAAATACCAACAGCTAACTGAATGTTAACTGTTGGCGTTTTCTGTTGAAGAAATGTTATTTCTTGTATTCAAGCCTATCTAAACGTTCGTTGATGGCTTGAAGGCTCTGAAGCACAACTTTAAAAGTTTCTCTAACTTCTACTTTGAAGTCTCTCAACTCCGCTTCAATGTTTGCGAACCTTCGATCAACCATGACCTTGTATTCCTCAAACTCTTTCCGGGTAACGTACTATGTTTTTGGCTTTTTCACTTTTCGTTGCCAACGTTCCTTAATTATAACATCAAAAGTTCCGTCTGGCAACGGAATCCAATTTGATTTTAATTCAAGAACCTCACACTGACTTCTAAACTTCTTGTATTCCCTTTCGGAATATCATTTGTTATTCAAAGTCAAAACCCCCTTTCTTACTTAAGTAATTAGAAAAAGAAGTAGACACTACCGAATTCCTTTTAGAAACAACAAATGTTGATGCGAGAACCAACGCAACAGATGCCTTTGCAAACGCGAAAGACTTATGCCCATAAGGGCTAATAAAAATGAGTTCATATGAGATTAAAGGGAGTTTTTACACTACCCAAGGTAATTAAATAGTGTTAAAGAACAATATAAAAGCCACCCGAAGGGTGGCTTTTCCATATACACAGAAAGCGAACTAAATAAATAGGTTGTCCCATTCCGCTAACTGCAGTTTTATTATAAAAGAAATAACTAACCTTCGGAGTGGCTTTCTTTCTATACTGAGCAGGAGGCGACTAAAATAGTTGAATAATTCTGTCTACTGCAATCTAGTTATAAAAAACTCACCTAAAAGGTAGTTATTAACACCAAAAAAGCTTTTTAGAAGCGTTCTTAGTTTCAATTAAATAAACTCAGTGAATTAATTAAGCTTAACCAACTAACTTAGGCTTTTTCTTGAAGATAACGTTTAAGTATCAACTTCATGGTTTTGAATATCTTCTATCAATGCTTAAAGGCGGTAGAAAACGTTAGCCCACTTGTACAGTGCATTTTCAAAGAACAGTTCATTACTACGCTTTACCAACATACTGATTTCACTGAGGTTAGTGTGCTTGTTTTGCATAGTTTTAATTTTGAGCTCATTCTTATGACTACTTCAACCCTCATAAAAGCCCTTAAACTGATTGTTAAACTTCGTAATAAAGTACTTAGCATCCTTGTACTTGCTCGCCTGTTTTTCAAAGTCTAAGTCCCTCTGTTCCTTGGTTTCGTAAAACTTAATTTCGAACTTCAAACTGTTTAAATAACTGTCCAACAACTTCTTTAAGATCTTGTCCTTGGTGGTTTGGTACAAGTTAACAAAGAACGATTGGAACACATAGTCATCTAACTTAACAAACGCTGGCAGCATCTTTTTGTCAAAGTGTTCGTTCTTGAGTAGTGGTAAGCACACCTGTTCTAAGGTTTTATAACCGTTGAAGTCAAACTGGTTTTTGTCCACTAAGTCCTTAATTCGTTGAAAGACAAACCAAAGGTTTAACTCGGTGGAAATACTCCTGCCATCGCTGTAGATTGATTGGTACATGTGGTGCCGGGTAATTAAGAAGTTTTCAATTAAGGGCAAGCACTTATCCTTAAAGAAGATTTCGTAAATCCCCTTGACCTTGACACAGTCCATCTCCTTAATAATCGTTTGGTAATCTATCAAGGAATGACTGGTACCCGTAAAGTAGGAATCGCGCAACAGGTAATCCATCCGGTCAGCATCCAAGTCAGATGAAATTAACTGGCGCATCCAGTAGTTGGATGGCTTTAACTCCTTGTTTTCATCAATTAAAGCAGCTACTAGTTTAGGATCAATCTGGTTCGCCTTTAAGATTGATTTAATCGGTTCACTGTTGACAAATAAGGTTGTAACTTCTTCGTGGATAAACAGCTCCTTCTTAAAGTTAGGGGCTTTGGCAAAGTAAATCTCAAAGGCATGGGAATGCGGTGCATGACCAATGTCGTGCAGTAATCCCGCTACTAACACCGTTTGCTTTTTGTTTTCATTAATCGGAGCAAACGCGGGGGTGTTTAAGATCCGCCTTAACAGTTCATAAACCCCCAAGGAGTGGGAATAACGCGTGTGCACGCCCCCCGGATAAAAATGAAAGTTAATTCCTAGCTGCTTAATGTTACGTAAACGGCGAAACGCTTCCGTTTCCACCAACTCATACATCCACTTAGTTTGTTGGTCAAACAGCACTTCCCCTAAAATGGGATCTTTAAAAAAGATTTGTTGCATGAATAAATTCTTGAAGCAGCGCTAATACCTGAGTCTTACCGTGCAGCACCATGATGCCCGCCAAATCAGGACCATGCTCTTTGTTGGTAAAGATCAACCGTAGTGGCATAAACAGCTTTTTGCCCTTCAGATTAAACTGTTCACCAATTTGGGTTAAGGTACTTTTGACATTGTCCTTCGTCCAATCAGGTAGGGCTTGGAGTTGTTCACTCAGTTGTTGTAGGGCTGGTTGGAGCTCTTTGAAGAGTTCACGGTGCTTAACACTTAAATGCTGCACCCCTAACTTGGGGGCAAAGGTTTCCTTAATCAGTTGGTTAATTTCAATGCCAAACGCTAGTTGGTTTTTGAACAACAGACTGACTTCTTTGCGCTTGTCCTTGAGGTAATCAAAGTCAATCGTTAAGTAGTTATCAATAAAGTTAAAGTAGGCATTATCACTCAACTCCTTAATGTAGTGCGCATTAATTCACTGGAGTTTCTTAATGTCAAAGAAAGCGGGGGCGCCCACTACTCGATTGACCGTAAAGCTTTCAATTAGTTGGTTGATGGTAAAAAACTCGCGGTTAAAGTCCGAATTTCAACCCAGTAAGGCCAAGAAGTTTACCACCGCTTCTGGTAGGTAACCCTCTTGTTTGAACTGTTCAATGAACTGGATCGTTTTGGTATCACGCTTAGACAGTTTCTTGCCACTCTTGTCGACTATCACCGATAAATGACCAAAGCGGGGAATCTTTTGATAGCCTAAGGCTTGGTTAATTGCTAGTTGGTAAGCGGTGTTGGAAATGTGTTCCGCCCCCCGCAACACATCGGTAATTTCCATGTCATGGTCATCAATGACTACGGCAAAGTTATACGTGGCAATGCCATTGGCCTTCAATAAAACAATATCGGTGAGGGAGTTCCCGGGAATAGTAATCTTGCCCCGAACCTGGTCGTTTCAGGCAAACTCAGCGTCCTGGTTAATCTTTAAGCGAATCGTGAAGGGTACTTGGTTGTCTAAGTTTGTTTGAATGTGCTTGGAGTGTAAGTTGCGACACGTTCCCAAATACTTCGGGGTTTGGTGGTGTTCTAAAGCCAATTGGCGTTCGTGTTCTAACTTTTCTTTAGTACAGAAACAACGGTAGGCCAAACCCTTGCCCACAAGTTCATAAGCTAGTTTTTTGTAGGTAGCCAACTTTTGACTTTGGATGTAAGGCCCATAATTACCCGGGTTGTAAATTGATTCATCGGGAATAATTCCTAACCAGCGCAAGTTTTCTAGTTGTGATTCAATTCCCCCTTCCACATTGCGCTCAGTGTCAGTGTCCTCAATCCGGATAATAAACTCGCCGTTAAAGTGTTTTGCTAACAAGAAGTTAAAGATAGCAGTACGCGCACCCCCAACATGAAGGTAACCGGTAGGGGAAGGGGCATAACGGGTTCTAATCTTTTCCATCGCTTAAATGTGGTTTAAGGCTAAGATACAAACGAAACAACTGGGTCGGGGTTAACTCTTGGGCTCTAACCGTTGCCGCCAAGTCCTGTTGTTGTAAAACATTAGTTAACAAGGTGGGTGGGAGAAGCTGCTTGAGGTTATTGACTAACAGCTTGCGGCGCTGGTGAAAACACTGTTTTAAGAACCGGCCAAAATGAGCTTCATAGGCTACTTGGGGTTTCTTTTTTAACAACATTAACACCGAATCCACTTGGGGCTTGGGCTTAAAAGCAACCTTATCAATTTTAAAAACTGATTTAATTTGCAAATGGTATTGGCAAAACACACCAAAGGCACTATAGTTAGGCGAATTTACCGGTGCTACTAACCTTTGACCAAACTCCCACTGGCACACCAGCACAAATTGTTGTAGTTTGGAAGCCAAAAAGTTAGCAATCAAAGGGGAGCTAATACTGTAGGGAATGTTGCCACAAAGCAAAGGTATAGTGTCAGGAAAATACTGATCCAAGTTTTGTTTTAAGGCATCACCAATGGTTAGTTGTTCTTCAGTTAAGATTTCGTTGACCAAGAGGTATTCCGCTAAGCGTTTATCCAACTCTATGCCATGGTATGGTAGTTGTAACTTGAGTAAAGCCTTGGTTAACGCTCCCTTACCCGGCCCCACTTCAATTAAGGCCGTTGGGTTGAGACTCTTAATTAACCGACAGGTTTTGGCAATTACACTCTGGTCAACCGTAAAGTTTTGCCCTAATTTGCGTGAAGGATAAAAACTATTCACGTTCTAACATTTGTTCTAATTTGGAATACGTTAACCGTCTTCTGGTACGCCTTTTAACAATAAAGAGGTGGGTTAGATAAGACTGGAGTAAGGTAAACAAGGCATTTAAGAACCAGTACACACCCACACCAGCGGCACTAAAAGCCGTAATAACAGCAAACACAAAGTAGAAAATTAACTGCATTCGCTTTGTTTTATTGAGCTGTTCAATGCTCTTTTGCGAATGCGCTTTAGCATTCTCATTGCGTTTGCTAGCCCAAATTTGGGGCAACTTTTGCGAGATAAATTGCACTGGTAAAACAATGATTAAGAAGATAATAAAGGTTCACCCGGTACTGGTAAAGTTCGAGAAGATTTCCGTTAACGGTACCTTAGATAAGTCCCAGAAGTTAAACAGGATAATCGCTTTAATTGGTCTTAAAGTCGTGACAATCCGGTAAATAATGAGGAAGATGGGTAAGGTAACAAAGACCTGAACAAAGGAAGCAGAAGACTTAATGTTGTGCTTCTTGTACAAGGACATGATCTCCATCTGGCGGTTGCGTTTGCTCTGTAAGTCCAACGCCCCTTTGTACTTGGCATTAATTTCTGCTAATTTGCCCTGGACCTCGTTCATTTTTTCTAACGCCAGCGTGGAGTTGAGGGTAATCACTATGGTAATTAACCGTACTAGGAACAACAACACAATCAAAGACAAGATCATGTTGAAGCCCAATTCCGGACCGCTACCTAGAGGAACCCTGGTAGCGTACATAATCGGCAACACTATTTGCGATGCTGGTCAGACAAACCAACCATAAAAGGGACCATACGCTAAGGTATAGTCACTAAAGGTAAAGTAGGGCCCCACATTATTGGACTGCAAGTCATACCGGTAGTCCCCCGTAGTACCAAAGTTATAACCAATTTCTAACCCCGAACCTAACAGTTGGTTGGTTCCCGTTCAGGGCTGTGCTAGGGTTTGGGTACAACCCCATAAACCTACTATGGTTAAAAAGATAAAGATCGCTACTTTAAGGACTTTAAAGATAATGCCCCAGGCCTTTTTGAGATTTTTGAGGCCGTTCTTTTCGTTAACAACGGCCGCTGATCAAAAAGGGTTAAAGGTGGTTTTGAGCTCTTTATGCTTCTTATTTAGGTTTAGGGGCATCGAGTTCTTGGAGCTTTTGGCAAAGGTTTAAAAAGATCGTTTGCTTTTCTTTAAATGTTAATTCAAGGAAGCCTTGATTAATAATAACCAAAACATCAACAGCGTTTAAATTGCAAAACTGTTCCCGCATGATCGCTTTAACTTGCCGTCGAATTAAGTTGCGCTCAACCGCTAACTTGTATTTGGTCTTTGATATCGAAACCGCTGCCCTTCAAGTGCGGACGCGGTTCGGCATAAAGTAGGCCATCAAAAAGGTCGAGAAGAAGCGTCTTTTACTCCTTAGAAGGGCAGCAAATACTTTACGATCACGGAGGTGGTGCTTGGTCTTAGCGCTCACTGGATACGGTGAGTTGGGCGCGTTGTTTTTTTCTTCTTAACTTCAATACTTTACGACCAGAAGCAGTGGCCATGCGAGCTAAAAAGCCATGGGTTTTTGCTCGCTTTAATTTACTTGGTTGATATGTACGTTTCATGATACAGCGCTAAGTATTATAACTAAGATAAAGGACGCACCAATTCGTTAGACACTATCTTACCATCGGAGATCTTAATGATCCTGTTAGCGATCCGCGTTAACGTTTCATCGTGGGTCACCATTAGAATAGTTTGCTTATATTCCTTATTGATTGCTTGTAATAAGGCAATAATGTTTTCGGCTGAATTACTGTCTAAAGCCCCCGTAGGTTCGTCACAGAGCAACAAGCGCGGTTCTTTAATAATGGCACGGGCAATCGCCACCCGTTGCTTTTGACCCCCCGACATCTCGTACGGAAACTTGTACAAGATTTCCTTAATGTCGAGTTTAGCCGCTAACTCCTCGATGTCTAAGTGGCGTTGTTCTTTAGGAATTAGGTTTTGCGAGATGGCAATGTTGTCATACGCACTTAAGAGTTCTATGAGGTTATAGCGTTGGTACACATAACCCAGGTTTAACTTGCGGTAAGTTAAGAGCTGTTCGGTGGTACATTTTTCCAAGGGACAATTGGCCACCACACAGCTGCCCAAAGAAATTGAGTCATAACCGCCTATTAGGTTTAAAAGGGTAGTTTTCCCCGAACCTGATGGACCTAAAATAATCACAATTTCCCCATAACCGATTTCTAGAGAAACATCGGATAAGATAGCAATCTGTTCCTTGGAGTCCTCCCCGTGAACGTGCTTTCACATCTTGTCAATCGAGATCACCATCCCTTGTTTTTGCTCGACATAAGGAGTGCGGTAGGGGGCTTCCAGTTTTTCCTTTCACTGGGAGATCAACCCACCCTGCTTTTTCGGTTTTTTGGGTTTAGGTGCCTTGGTGGTCTTCGTGGGTTTAACTTGTTCCGGTTTAACTGCTTTCGCCTTCGGTTCCTTAACTTTTTTCACCTTAGCGGGTTTGGCCTTCTTTGTTTTCTTGTCTGATGCTAAATAATACTCAAAGGTGGCCTTAAAGCTGTTGGCCTTAACGTTTTCTAATATATCAACACACTCGTCCCAGACAAGTTCTTTCTCGGTTTTCTTTTCTGTATCTTTCATCCTAGAAGCCCTCCTTTAGTTCCGCTGAACGCAGTTTAATTCAGAAGTAAGAACCTATTACAAAGATGGTAGCACCAAACAGGACTATCCCCACCATGTAAGGTAAGAATTCAAACACGTTGGTTAAAAAGACCTGGGCAACCCCAAAGACTAGGGCTTGTACTCCTACTAGTAACCCAGCTAAGATAGCAATCGAGATTAATAACGAAAGTACAAAAGCGGGAATAAAGAAGCACAAGAGACTAACTAAGTTTTGACTATCTTTGTAACCAATCGATTTTAAGGAAATAAAGATCCGTTTCATTTCCTCTAACATCGAGATCCCCAAGACCACACAGGTAATAAAGATAAAGGGAATAATGGTGCCCAACAAGAACCCATCAACTTGGTTCACGGTATTAAACAACACCTCAGCATTATTCCTGATAATTTGAGCACTAGCACCAGCGGCATCTAATTTGAGAGCTGGCACCATGATGTTATCGCCAAAGATACCTCTAGCACCACTAGCTAATGATTGAGTTGAAGATCATTCGCTCGTATATTTTTCGATAACCTTTTTTCTGAGGTTTTCATAGGTCATGCTATCCACACCATTCTCACCCTTTTCCTTAGCCAACTGCTTTAAGATATCCGGATCACTTGGTAAGATTGAGGTAATAATCTTGTTTTCCTTACCCTTAACGAGGTTAGTAAAGTTATTACCAAAATCCGTAAAGTTACCCCAGACACCAATGTTGGAGTTAAGTACTAGCGCTTTACTTACCAAAGAAGGGTTGAGTTCCTTGGAGAAGACCCCGTTAAAGGGAACATAACCAGTTGGCTTGACGTACTCAATCTTGTACTTGTCTTTGGTGGTAGAAGAGGTACTTCCACCCCCACAAGAGCCACCGCCAGACGAAGTACCACCGCTATCATCGGTGGATTGGCCGTCCTTCACCACATCCTTAATGACCCCGCGGCCATCAAAGCCATTGAGCTTTAACAAGCGGTTGGCAATGTTTTGGTTAATATACAACTCTTGGCCAAAGGCATCGGTAGATACCCCGACAACCCGGAACTTCACGGTTGGTGTTTTAGGTGGGCTCTTTAAGAACTTCTCTGAATAGCGGGTCGCAGTGTTCTTGGGTTGAATAAAGATGAAGTCCCCGATCTTGGTGTTATTAGCGTACTGAAAGCCAGCGTTGACAATGACATTCATCTCACTGTCAGAGCTAATTAAGCTCCTCAAGGAGTTACCACCGCTGTCAGTGAGGTTGACAAACTTGGAATCGGGACTAATTCCCGTTAAGGTGTAAGGGGAATCAGGATTCAACTCGGTTTCCTTAAAACCAAATGGTCCTTGAACATAGGTGTATGTCTCCTCTATTTGTGGATCAACCGGGACAATCCCGTACGTTAACTTCGAGTCTTGGTAGGACAACTCCGGATCACCATAGACCTTAGTAAGGAATTTAAGGAATTGTTCGTTAAATTTAACTCCACCAGGATTGAAGACGTTAATCTGGGTTAATACCCTAGCGGCATCCAACTGAATAAAGTTAGTCCGAGGGTTGCGGGTTAAGAAACCATTATTGTTGAGGTTATTAAAGTAGTCATCAGTAAGAACCGCAATTAAAAACTGGTCACTGGAAGCCGATAACAATTGGGTAATTTGTTTAGGAATGGACTTCTCGACAATTTCCCACGGGTTAATGTTGGCACCACCAACGCGAATTGGGAAGTCAATGAGCCACTTCGACAACGAAGCATGACGGAAGAAGTTTTCGTCAGTGTTTAATTGTTGGGCACCACCAAAACTTGGGAGAAGGAGGTTACCGAGTTCGATTTCCTTACCATTATGCTTGATTGGGTTTCCGCCCCTATCACTAATCTTTAACTCTTTGAGGTTGTATGGGTGTTCTTTGTCGTTGCCTTGCTGTTGTTGCTGCTGTTGCTGTTGCTGAATTTGATCACCCTTATCTTTATACAAGGCCTTAATCCCCAAACTATCATCAGTTTGACCAAACCGGGAGTAGGGTTGGATTGCATACCAGCCGGATTGTTCTGTGGGGGTTTGCAAGTTCAACCTATAGTTAAAGTTGCGGTTTAAAACGGTGTTGGACTGCGCTGCCCCAAACTTTTGCGGGATGGTACCGGCAATCCCAATTAAAAGCAGGGCAATCGAACTTAACCCCACATAGGTAAATAACCGCGATAACCGGGAAATAATCAAGGACACCCGGAATTTACCCAAGGGAGTCATGGTGTAACTCTTGCGCTTTAATAAGTGCAAGAAGACCGAGAACTTGGTTTCATTACCCTGGTCAATCAGGACATTAACCGGTTTACTAAAGAGCTGTTTAAAGGCTATTCAGCTAATAAACTGGAAGATCACAAAGATCAACAGCATTGATCCAAAGAAGGAAATCCAGTTAAAGCTGTTGGCCGGTAGGGCTATAAACCAATACCTGGAGATAATCCCCTTAACTTGTCTTTCTAAAAGGAAGGCAAATAAGACCCCAAAGAAGGATGAGGCCAAGGCTACTATGCCGGCAAACACACTCATGCCGGCAATAAACTTGGCTGTGGAGAACCCACCAGCGCGGATAATTGAGAACTCCGTTTGGTTCTTCTTAATAAAAGACTTAATTAAGAGAATGGTGAGGTATAAGGCCAAGATGGATAAGAAGGCCGCAATGACCACACTGGCAATGGCTAAATAGTTCTGCACTAAGTCCGGGAAATAGTTCCGGGCTGTAGTTAAGTACTGGAACTTGGAGATGTCCTTAACGTTAAAGGCGAGATCACTGTCAGGGACGTTGAGATAGCCCTTTAACTTAGAGGCCACCACCTGGATCGGATCTTGGCCAGGGAACTTCGCTTTAATGTCATCCGACTTAAAGGCGTAGTAGTTCTCCTCACTAGCAGCAGGCACAGCAAACAAGACACTGCGGTATCCCTGCTCATTGAGAAAGATTAAGCCTTCATCCTGGGAGTTGGGCAGCGGTGAGTTTGTGGACAATACCGGATAACCGGTTTCTACCGACTCCCCAACCCCTAGAATGGCAAAGGTTAACCCGTTAATTGAGAACTTGTATTTGTCCGGTAAGTCATTTAACCAGTTGGGTAAATTCTTCGGGTTTTCTAACGCTTCTTTTCAGGAGTATTCCCCTTCTAAAGGTAATACTTCCTTGTCGTGTTTAAATAACCATTGCTGGGATACCACCGCACCGTGGGCTTCCGGGGTTTGGACTACCAAGGACTGTTGGTCGGTGGGTAGTGTTAACGGGTTAACCCCGTTTTTAAAGATAATCGGGATATCTAACTTTTGGCGGTTAGTTACTACCAGTTCATTCGGATCTTGAAACTCCGGGGTAAAGTAACTCAATAAAGCAACATAGTTGTTAGCGGCTTTACTCTTACCTGTATCATCTAAAGCCTCAAAGCCTTTTTTATCTAATGTAGACCACTGCTTAATGGCATTGGTTAAGGTATCAGGAATACTGGAAGCCTCGGGGAGTTGGGCTTGGGCTAAGATGTTAGCTAGTTTGGGGTAGATTTGGAATTTGGTAGAAGTACTCTTAGTATCACCAGTTGAAGAGGAGCTGGAGGAAGAAGATGCATCTGCTAACTGGGTTAAAGAGTCCGTTGTGCCTTGCGTAGTTTTGGCTGCTGCTTTTTTCTTTTCATCTGTTTGAGTAGGCTTAAACTCATACTCAACCTTTTCCAAGAAGCCTTCACTGTTACTCTTGCCCCCATATACCATGTCTAAAGGATTGCTTTTTTCCTTGGCAAGCCAAGACTTAAAGTCAATCCCTTCAATAATAGAAAGGTCGTTGTACTTCTGGGAATCCTTATTGGAGACCAAGAAGGAAGCGGATTCTTCGGAGCTAACATTGGTAGCAATCGCTAAGTTTTGCCGGGAAAACTCCAATTCCTTTAAGGCTTTCGCCTTTAAGAAGGCATCGCGGTATTTTTCTAATAACGCGGTGACCTTCTTCACCAGGTCTTCCTGGAAGATGCTTTTTCATTGGTGTAAAAGGGCAGCATAAACGTTATTTAAATTCTTTTGCGGAGTGTGGTTTTCGGTGCCAATTTTGTCGAATTCAGTTTGGACATCACTAAAAGTTGGATCTTTTACACCGTCATCTGCACTACTTTCCTGCTTATCTTGTTGTTGCTGAGCCAACGCAACGTTCGTTTGTTGTAATTGCGGCTTGAAAGAAACAGAAGAGCGAAGTGCAATCAAGCTAGAGGGATTGCTCTGAGTGCCAGACTTAGCATCGCCTGATTGTGTACTAAAAATCGTATTCTTATCACTATTACTAAACCCCGGTAAAGAATCTAAGATCGTTTCCTTTTCGGCTTTAACCTTATTTTTGAGCCAGTCCTTATTGATGTTAAGCTCAAACGATTTGAGGCTATTAATCTTCTTCTTGAGGTTATAGTCACCAAAGGAAGAACCGCTAGAAATAGTTTCTGCTTGCTTTTCTAATCAGTCAAATTCTTCCTTATCTAAATGAGATAACTTACTTGTATCTAAAGTAAAAGTTAAGGAGAAAGATTGTTTTTCTTTTTCCTCTTTTTCTTTTTGGAAGACCTCTATTATTTCTTTAAAGGGATTAGTCTCTAACCAAAACTGGGAACCCGGACTAATCGGGAGCACAATAGGTACATTAGCGTACAGATTGGGAAACCAGTTAGCAGCTTCTTGCCCTCCGGTAAATAGATAAGAGGTACTTAGTTTACTGGTTTCGCTTTCTCCACTGCTACTACCAGCTTGAGTTTCATCACTTTTAAACCAAACTTTGTCTTTATTATTGGTAGTGTATTGTTCTTCTACATTACTATCTTTTTTGGGCTCCTCGTTATTTGTGGTTTCTTTAGCATCAGTCATCTTGGCCTGAGCTGCTAACGGTTGTAAAACTGTTGTGGCACTACTCGCTTGCTTGATTTCTGATTCTTTTTTAGTAGTTTTCTTATCCTCACCCTTCCCTTTATCACCAGGAAAGCGAGCAATCCCAGCGCGCAGGGGAATCCCATCCAGAATTAAACCATCGACAAAGTAACCTAAAAGGTTACCTTCTTTGTCATAAATCAGGTTTTGGGGATCGGAAACCGTATTTTGGTTTAAGTTATTACTTTCTTGATCGGTTCCAGTAAAAGAATAGGTATTTTGAAAGGAAACAGCTAAACCCTTATAGATCCGTTTTTCGGCAATGTTGGCTTCCCCGTAAGCTTTCAAAATGCCTCTTTTACGAGCTAAAAAGAGTTTGTCTTTATCGTTAACATCACCTTTTCGTGGGTTATTATCTGATGATTTGGTGGTTGAATCTTTCTTTTCAGAATCACTCCCAAAATCAGATTCTTGGTAGGGCCAGATAAAGTTAATCTTCTTTTCGGTTAACTTGATTTTGGAAAAATGTCTTTTTTCTTCTTTAAGTTCCGTTTTATACACCGGACTACTTTCTTTAAAGATAGCCGGTGCTACTATGTCAGAAGAATTCGTTTTTTGAACAAGGGCAGTATAAGTATTAACAAGGTTATTACTTAACTGAAGAATCGACGTTTCCAGGAAGATAATGCTGAAAAGGACGAAAATAATCCCAAAAAGTAAAAAGAAGAACTTTTTGAGTGACTTAAAGATCTGTTTAAAAAACGAAAACATTTAGTTTAAACTCAGTTAATTTCGTTAATAGTCTTGGGTCTTTGGTTAAGATAATCAGCCACAATCTTGCCATCGTGGATCTTAATGACCCGTTTAGCTAGCTCCACAATCTTTTCATTGTGGGTAACTAATACTATAGTAGTGCCCTTATCGCGGTTATACTCCACAAAGAACTGGAGGATCTTTTTGGAAATATCCACGTTAACAGCACCAGTTGGCTCATCACCAAATAAAATACGCGGCTCTTTGATTAGTGCCCGCGCAATTGCTACCCGTTGTTGTTGTCCCCCAGAGAGCTTGGTAATCTTTTTATTACGGTGCTCTTTGAGCTCCAACCGCTCTAACAGCTCTTCCAAATTGTTGTTATGGCGCTTTTTAAAGGGAACTGCCAGTTTAATGTTGTCATCAACGTTCAGATCCCTTAAAAGGCCATACTGTTGGAATATATAACCTACGTTTTTATTCCGTAGTGATGTTAATTGGGCATCATTACAACAAATAGTACTCCTACCACACACAAAACTCACCCCGCTAGTAGGTCGGTCTAGGGCGGAGATTAAAGAGAGTAAACTAGTCTTACCAGAACCAGATTTACCCAAAATAACCACAAACTCCCCAGGGGCAATTTTGAGATCAATGTGATCACAGATTAACTGGTTTGTGATCCCATTGGTCACTGCCTTACAAACGCCCTTGAACAAAACATCGTAATGTTTAGCCTCAGGGTCTTTGTAGTGCCGTCTTTTAGGGTGCTTCTTATTTAACTTAACGATTAGCTTTGACGCTTTTTTACTTTGACAAAAATCGGGTCATTGCAAACAATAATCGAGGCTTCGTTTATTTTTAGCATTTAAAGACATTGAAACATGTCAGGTTTAACAACGGGACAGGCCAGTTTCAATTAAACACTTAAAGAATTATAAAAGGACTAAATTACCAAGGAAGTGATAATGTCTTGGAGGGAGTCGTCCTTCTCAATCATTTTAGCAACCCTTTTAACTGCAGCCACCACTGTAGAGTGGTTCCTTTTAAAAATTTTGCCTATTTGTTGGAACTGCATCTTGTACTTTTCCCTTAAAAGATAGTTACAAACGTCACGAACACGAACTAACTCTGCTTTACGGTTCTCAGACAAAACACTGTCCACCGGTACATTAAAGCGGCGGCAAACGTTTTCTATTAATAAATAAGGATCAAAGCTCTTTTTGTGGAACTTCTCAAATTCCTCAAACAGGATTTCCTTTAGATTGTCGTTGTTAATCAGGTTTTGCTTAGTTGTTTTGACATAAAACAGCAACTTAGTCGCAATCCCGTTTAAAGAACGAACATCGTTACCAGATATTTGCGCTGCCTTTTCGCGTGCTTCATTGGTAATTTGGATGTTGGGATCCTTTTCTTTGAGCTTAACGGCCAAAATTTCACACAGGGAGCTCAAATTGTGTTTTTCAATCTTTAACAAGAGTCCCGATTTAAAGCGTGATACCATCCGTGGATCGATGTTGGCCAACTCATCGGGGGTTTTATCGGACACAAACACAATTTGCTTGCCCTTACTTACTAAGTTATTAAAGATGGAAAAGAGGATCTCTAAGGTTTTTTCCTTTTTCCCGAAGATCTGAGTATCGTCAATTAAGAGTAAATCCAATGACTCGTAAGCAGCCTTGAGCTTTTCAATGCCTTGATCCTTGTCCTTTTGGTAAAAGGCATTGACAACCTCCTGGGCAAAGTCACTCGATACCACATACTTTACCTTAGCGTTGGGAAAGTGGAAGAACTTATCATTCCCAATCGCTTGGAGTAAGTGGGTTTTACCCAGACCAGTCTCCCCATAGATAAATAAGGGGGAGAAATCAGCGTCCTGAGTTTCGCTAATCCGTACACCCGCTTCATAAACCCGGCGGTTGCCATCACCAACCACAAAGTTTTTAAAGGTAAAGTTTTTGCTTAGCCCCGAACTTTGGTAAAGCGTTTCGCGGTTATCTTCCTCTAGTTTGGCTAAATTGATAAAGAAGTCCTGTTCGTTGACAAACCGAACTGCTCTAATGTCATCGTACAGTTTGCTAGCTAACCCGACAATATCAGGGTTGCTATTGAGGGTTTCACGGGCAAACTCATTGTCCACAATCACAAAAAGGACATTATTTTTTAAAGCAAACCGTTTAAGATTCTTAATGTACTTGTCATAAAAACCTAAAGTAGTTTCGTATTGCTTTTTTAAAAGAAGTTTAAAAGCACTAAATTGTTCCATTACTCTTCTATTACCTGATCAAGAATCATCGTTAAGATCCGCGAAATCGGAATGTTAACCGCTTTAGCATAACGCTTAACCTTATTCCAAACACGGGTACTCGTATAAAAGTGCACCCCGGTGGTTAAGCGTTCTTGCCGTTCTAAGGGAATAATACCGGGCACTTTCGCCTTTGGTGGTGGTAGATTTTCTTCTTCCTGCAATTTATTAGATGGGCGAGTAGTCTGCTTAACTTGTGGCTGTTCAACCGCTGGTTGTGGTTCTGGTTGGGGAACAGGTTCCACAATTGGTTGCGTAGCCTCCACCTGAATATTAGATGTAGCTACTGGTTGTTGATTAAAGTTAGTGGTTGTAGGTTGTTGAAACTCATTTAAGTGCACTTCTGCAGTTTGGTGAACTGGTTGGGTTGGAACCTGATTATTAAAGTTAGTTGGTTCTGATTGCGGTTGCACATTAACTGGTTGGGTTGAAGTTTGTGGTTCTTGTTTGGGAAACTGGTTAAAGGATTTCACAAACTCATCGCCAAACTTGAGTAATGGTCCAAAATCAAACTGGGGCATGTCCTTAGAAAAGAACTTTCTGTCTTGACTAGATTGATTTGCTTTAGTTTGGTTAGTTAGATCATGTTGGGGTTGGACAACCGGTTCTAGTTTTAATTGTTGTTCTACCTTTTTAACTTCTTGAACAGTTTCTGCATCATCTTCATCCTGAGCTAGGTCAAAGAAGAAGCGTTTCTTTTTACTGATGGCCATGGTTGACTAGGTTAAGAATTTCTTTAGTAATCTCTAAATATTCAGTTTGGTACTTCTTGTTGGATGTTGGTGATACCAAAGAGATAGGTAACTTTTCATAACCAACAGCAGCAGATGACTTAGATGTTAAAGATACAAAGTGTTCTGAAAAAGCAACGTTGTTCTTGTGCGCTTTTGATTTCACCAACTCAATCACATCATTGTGTAATCTGGTGCGCATATTGACCTTGGTTGGTACCAAAATGGTTTTTAAGTTGGGGTTCTTTTCCTTAAAAGTATCAATCGTTTCGACAATCCGCATTAAACCCAACATAGAGTATTGATCAGGTTCAAACGGGATCACAATCACATCAGAGAGGCTCATGGCTGTGGAGACCAAGGTAGCCATGTTAGGCGGTGTGTCTAGTAAAACAAAGTCATACCTGCGGGTGAGTTGTTTAACTATTTCCGCAATATCAGAAGCCTTGTACTTTTTGCGAGCAATATCAATGTCCGCAAAATTTAACTCAAAGTTACAAGGCAAAATGTCTAGTCCTTCATAAACGGGCAACAAACAGTCATCAATTTCAATTGAACTGTTGGCGCCGTTAAACTTAGGCACTTTTAACAAAATATCAATTAAGGTGTTATTTAATCTTTCTGGATTTTGTCCAAAAGAGGCTGAAACGTTGCCCTGACCATCAAGGTCTAAAATAACTTTCCGTTGTTCAGGGCACAACTTTACAAGCGAACCAGCAACGTTAGTGGCCATGGTTGTTTTTAAAACGCCACCTTTGTTATTAACAAAAGAAATAATCATATGCGCGCGTATAAGTTTGCTTAAATTCTAATATTTATTTAGTATTATTCCGGTAT", "length": 811088, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_000143945.1|CP002077.1", "orig_description": "HMT-732 Mycoplasmoides pneumoniae FH" } ], "run": { "start": "2026-01-26 08:56:13", "end": "2026-01-26 09:03:35", "duration": "7.37 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }